CN203866318U - Full-automatic nucleic acid detect detection and reaction device - Google Patents
Full-automatic nucleic acid detect detection and reaction device Download PDFInfo
- Publication number
- CN203866318U CN203866318U CN201420201952.5U CN201420201952U CN203866318U CN 203866318 U CN203866318 U CN 203866318U CN 201420201952 U CN201420201952 U CN 201420201952U CN 203866318 U CN203866318 U CN 203866318U
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- China
- Prior art keywords
- nucleic acid
- movable arm
- overhead gage
- conductive rod
- magnetic conductive
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- Expired - Lifetime
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- 150000007523 nucleic acids Chemical class 0.000 title claims abstract description 60
- 102000039446 nucleic acids Human genes 0.000 title claims abstract description 60
- 108020004707 nucleic acids Proteins 0.000 title claims abstract description 60
- 238000001514 detection method Methods 0.000 title abstract 2
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 40
- 238000000605 extraction Methods 0.000 claims abstract description 40
- 239000007788 liquid Substances 0.000 claims abstract description 22
- 239000011148 porous material Substances 0.000 claims description 4
- 238000012864 cross contamination Methods 0.000 abstract 1
- 238000005070 sampling Methods 0.000 abstract 1
- 238000010586 diagram Methods 0.000 description 5
- 238000011109 contamination Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000009434 installation Methods 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 239000011324 bead Substances 0.000 description 2
- 238000010276 construction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- JEIPFZHSYJVQDO-UHFFFAOYSA-N iron(III) oxide Inorganic materials O=[Fe]O[Fe]=O JEIPFZHSYJVQDO-UHFFFAOYSA-N 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model discloses a full-automatic nucleic acid detect detection and reaction device which comprises a housing, a reagent allocation mechanism and a nucleic acid extraction mechanism, wherein the housing comprises a frame, an upper baffle, a left side plate, a right side plate, a front baffle, a back baffle and a front door panel; an ultra-violet lamp is mounted on the inner wall of the upper baffle; the nucleic acid extraction mechanism comprises a platform bracket, a vertical guide rail, a magnetic-conductive rod and a magnetizing apparatus; a deep hole plate and a magnetic bar casing pipe are placed on the platform bracket; the magnetic-conductive rod moves up and down along the vertical guide rail; the magnetizing apparatus is arranged below the magnetic-conductive rod; a feed-through hole, in which the magnetic-conductive rod can be inserted, is formed in the magnetizing apparatus; the reagent allocation mechanism comprises a working platform, a movable arm, a liquid relief head and a transversal guide rail; a reaction reagent, a sample and a micropore plate are placed on the working platform; the movable arm moves left and right along the transversal guide rail; the liquid relief head moves front and back along the movable arm and can move up and down on the movable arm. As the movable arm and the liquid relief head are used for sampling and nucleic acid transferring, full automatization of a nucleic acid extraction process is realized, the efficiency is improved, and cross contamination among samples can be avoided effectively.
Description
Technical field
The utility model relates to the medicine equipment that is applied to in-vitro diagnosis, belongs to especially a kind of Automatic nucleic acid Testing reaction unit.
Background technology
Disclosed Automatic nucleic acid Testing device is the Automatic nucleic acid Testing reactive system that Hamilton company of Switzerland produces mostly on the market at present, consist predominantly of reagent distributor gears and nucleic acid extraction mechanism two portions, wherein reagent distributor gears has an application of sample platform, on this application of sample platform, is placed with deep-well plates.When described Automatic nucleic acid Testing reactive system operation, the liquid head that moves in reagent distributor gears is filled into reaction reagent in deep-well plates, and the mechanical manipulator that the deep-well plates after application of sample is carried by system is transferred in nucleic acid extraction mechanism and extracted.This reagent distributor gears and nucleic acid extraction mechanism are realized and being integrated and connected by the frame of bulky complex, not only complex structure, and also it is large to take up room, and installs and also has inconvenience.
In addition also there is certain problem in the system of this structure, in nucleic acid extraction effect:
1) owing to adopting mechanical manipulator to shift deep-well plates, cause device structure complexity, need reserved larger space, and manufacturing cost is higher, the cycle of whole nucleic acid extraction is longer, inefficiency;
2) shift in the process of deep-well plates at mechanical manipulator, in deep-well plates, be loaded with sample and reaction reagent, because the sample of nucleic acid extraction comprises the positive and feminine gender, in therefore shifting, be very easy to cause crossed contamination between sample, have a strong impact on the final effect of nucleic acid extraction.
Utility model content
Technical problem to be solved in the utility model is to provide a kind of Automatic nucleic acid Testing reaction unit, can simplification device structure and detecting step, and improve nucleic acid extraction efficiency, and effectively stop the problem of crossed contamination between sample.
For solving the problems of the technologies described above, the Automatic nucleic acid Testing reaction unit that the utility model provides, comprises casing, reagent distributor gears, nucleic acid extraction mechanism;
Described casing comprises frame, left plate, right side plate, overhead gage, rear deflector door, front apron and front door, described left plate and right side plate are separately fixed at the two ends of frame, described rear deflector door is fixed on the rear side of frame, between the top of described left side plate and the top of rear deflector door, be fixed with overhead gage, between the front side of described left side plate and the front side of overhead gage, be fixed with front apron, the bottom of described front apron is movably installed with front door, and the inwall of described overhead gage is provided with a ultraviolet lamp;
Described nucleic acid extraction mechanism comprises a platform support, a vertical guide, a magnetic conductive rod, a magnetizing apparatus, on described platform support, be placed with the magnetic rod sleeve of holding the deep-well plates of reaction reagent and sample and coordinating with magnetic conductive rod, described magnetic conductive rod is arranged in vertical guide and along vertical guide and moves up and down, and described magnetizing apparatus is positioned at the below of magnetic conductive rod and has the communicating pores inserting for magnetic conductive rod;
Described reagent distributor gears comprises that a workplatform, a movable arm, move liquid head, a cross slide way, on described workplatform, be placed with reaction reagent, sample and microwell plate, described movable arm is arranged on cross slide way, this movable arm moves left and right between workplatform and platform support along cross slide way, the described liquid head that moves is arranged on movable arm, and this moves liquid head and moves forward and backward and can on movable arm, vertically move up and down along movable arm.
Further, the end of described magnetizing apparatus is provided with radiator fan, and this end is connected with guide duct.
Preferably, described overhead gage is divided into the first overhead gage and the second overhead gage, and wherein the first overhead gage is positioned at the top of reagent distributor gears, and the second overhead gage is positioned at the top of nucleic acid extraction mechanism, and described ultraviolet lamp is arranged on the inwall of the first overhead gage.
Better, described front apron is divided into the first front apron and the second front apron, and described rear deflector door is divided into the first rear deflector door, the second rear deflector door and the 3rd rear deflector door, and described front door is divided into the first front door and the second front door.
In said structure, described magnetizing apparatus is hollow structure.
The utility model compact construction, easy for installation, low cost of manufacture, pass through cross slide way, movable arm and move liquid head and realized being well accurately integrated and connected of reagent distributor gears and nucleic acid extraction mechanism, utilization moves liquid head and directly the reagent in reagent distributor gears and sample is filled in the deep-well plates of nucleic acid extraction mechanism, save existing installation and completed the deep-well plates transfer step after application of sample, and the nucleic acid after extracting can be by moving liquid head fast transfer to the microwell plate of workplatform, really realize the full-automation of whole nucleic acid extraction process, greatly degree improve extraction efficiency, and effectively stop the problem of crossed contamination between sample, and then ensure again extraction effect.
Brief description of the drawings
Fig. 1 is forward direction schematic diagram of the present utility model;
Fig. 2 is backward schematic diagram of the present utility model;
Fig. 3 is partial schematic diagram of the present utility model, shows the position of ultraviolet lamp;
Fig. 4 is the overall schematic of reagent distributor gears of the present utility model and nucleic acid extraction mechanism;
Fig. 5 is the schematic diagram of reagent distributor gears of the present utility model;
Fig. 6 is the schematic diagram of nucleic acid extraction of the present utility model mechanism.
Wherein description of reference numerals is as follows:
1 is left plate; 2 is the first front apron; 3 is the first overhead gage; 4 is the second front apron; 5 is the second overhead gage; 6 is right side plate; 7 is the first front door; 8 is the second front door; 9 is the first rear deflector door; 10 is the second rear deflector door; 11 is the 3rd rear deflector door; 12 is ultraviolet lamp; 13 is movable arm; 14 for moving liquid head; 15 is workplatform; 16 is platform support; 17 is deep-well plates; 18 is magnetic rod sleeve; 19 is magnetizing apparatus; 20 is magnetic conductive rod; 21 is guide duct; 22 is cross slide way.
Embodiment
Below in conjunction with accompanying drawing and embodiment, the utility model is described in further detail.
The Automatic nucleic acid Testing reaction unit that the utility model provides, comprises casing, reagent distributor gears, nucleic acid extraction mechanism, as shown in Figures 1 to 4.
Casing comprises frame, left plate 1, right side plate 6, overhead gage, rear deflector door, front apron and front door, described left plate and right side plate are separately fixed at the two ends of frame, described rear deflector door is fixed on the rear side of frame, between the top of described left side plate and the top of rear deflector door, be fixed with overhead gage, between the front side of described left side plate and the front side of overhead gage, be fixed with front apron, the bottom of described front apron is movably installed with front door, and the inwall of described overhead gage is provided with a ultraviolet lamp.
Install and the transport of equipment in order to facilitate part in device, in the present embodiment, as shown in Figure 1, Figure 2, Figure 3 shows, overhead gage is divided into the first overhead gage 3 and the second overhead gage 5, wherein the first overhead gage 3 is positioned at the top of reagent distributor gears, the second overhead gage 5 is positioned at the top of nucleic acid extraction mechanism, and described ultraviolet lamp 12 is arranged on the inwall of the first overhead gage 3.Described front apron is divided into the first front apron 2 and the second front apron 4, and described rear deflector door is divided into the first rear deflector door 9, the second rear deflector door 10 and the 3rd rear deflector door 11, and described front door is divided into the first front door 7 and the second front door 8.
As shown in Figure 6, nucleic acid extraction mechanism comprises a platform support 16, a vertical guide, a magnetic conductive rod 20, a magnetizing apparatus 19, on described platform support 16, be placed with the magnetic rod sleeve 18 of holding the deep-well plates 17 of reaction reagent and sample and coordinating with magnetic conductive rod 20, described magnetic conductive rod 20 is arranged in vertical guide and along vertical guide and moves up and down, and described magnetizing apparatus 19 is positioned at the below of magnetic conductive rod 20 and has the communicating pores inserting for magnetic conductive rod 20.
Reagent distributor gears, as shown in Figure 5, comprise that a workplatform 15, a movable arm 13, move liquid head 14 and a cross slide way 22, on described workplatform 15, be placed with reaction reagent, sample and microwell plate, described movable arm 13 is arranged on cross slide way 22, this movable arm 13 moves left and right between workplatform 15 and platform support 16 along cross slide way 22, the described liquid head 14 that moves is arranged on movable arm 13, and this moves liquid head 14 and moves forward and backward and can on movable arm 13, vertically move up and down along movable arm 13.
Magnetizing apparatus 19 is hollow structure, and in nucleic acid extraction process, magnetizing apparatus can produce heat, therefore its end is provided with radiator fan, this end is also connected with guide duct, is mainly that the air-flow that radiator fan is produced imports to device external, prevents device interior air strong convection and affects nucleic acid extraction.
In the present embodiment, the lower end of magnetic conductive rod 20 is provided with 96 magnetic conductive heads, therefore can process 96 samples simultaneously.96 magnetic conductive heads are mentioned in process at nucleic acid, and magnetizing apparatus 19 can make magnetic conductive head produce magnetic field, and the magnetic bead in reaction reagent is adsorbed on to its surface.Directly contact with magnetic bead in order not try magnetic conductive head, therefore in device, have the magnetic rod sleeve supporting with magnetic conductive head 18, it not only can prevent reaction reagent corrosion magnetic conductive head, and can prevent iron rust on magnetic conductive head or bonding foreign substance pollution reagent.
In Automatic nucleic acid Testing reaction unit of the present utility model, required each reagent, sample and consumptive material is placed on the workplatform 15 of reagent distributor gears, and deep-well plates 17 and magnetic rod sleeve 18 are placed on the platform support 16 of nucleic acid extraction mechanism.
When this device work, moving in the deep-well plates 17 on the platform support 16 that liquid head 14 directly adds sample and reaction reagent to nucleic acid extraction mechanism on the movable arm 13 of reagent distributor gears, after application of sample finishes, platform support 16 in nucleic acid extraction mechanism along X-axis move to specified location make pending deep-well plates 17 be positioned at magnetic conductive rod 20 under, magnetic conductive rod 20 moves to specified location along Z axis, magnetic conductive head is arranged in the communicating pores of magnetizing apparatus 19, described magnetic conductive rod 20 and magnetizing apparatus 19 coordinate running, effectively the reagent in deep-well plates are carried out to nucleic acid extraction.
After nucleic acid extraction finishes, platform support 16 moves to specified location along X-axis, nucleic acid substances in deep-well plates 17 on the platform support 16 of nucleic acid extraction mechanism is transferred to (not shown) in the microwell plate on reagent distributor gears workplatform 15 by the liquid head 14 that moves on the movable arm 13 of reagent distributor gears, and so far whole nucleic acid is mentioned end of processing.
Moving in the deep-well plates on the platform support that liquid head can directly add needed each reaction sample to nucleic acid extraction mechanism in the utility model on the movable arm of reagent distributor gears, can realize and efficiently move fast liquid function, and after nucleic acid extraction completes, moving liquid head is pipetted into the nucleic acid in deep-well plates in the microwell plate on agent distributor gears workplatform, whole leaching process has really been realized 100% full-automation, need not be by the artificial nucleic acid substances shifting after extracting.Magnetic conductive rod contains 96 magnetic conductive heads, can realize 96 increments are originally carried out to nucleic acid extraction simultaneously, large degree ground has improved extraction efficiency, and effectively stop the problem of crossed contamination between sample, and then ensure again extraction effect, and the utility model compact construction, easy for installation, low cost of manufacture, the scale of carrying out in reality that is simultaneously highly suitable for is applied, and has extraordinary market application foreground.
By specific embodiment, the utility model is had been described in detail above, this embodiment is only preferred embodiment of the present utility model, and it not limits the utility model.In the situation that not departing from the utility model principle; equivalent replacement and improvement that the composition of the shape of those skilled in the art to magnetic conductive rod and magnetizing apparatus, casing, shape and the structure etc. of movable arm are made, in the technology category that all should be considered as protecting at the utility model.
Claims (6)
1. an Automatic nucleic acid Testing reaction unit, is characterized in that, comprises casing, reagent distributor gears, nucleic acid extraction mechanism;
Described casing comprises frame, left plate, right side plate, overhead gage, rear deflector door, front apron and front door, described left plate and right side plate are separately fixed at the two ends of frame, described rear deflector door is fixed on the rear side of frame, between the top of described left side plate and the top of rear deflector door, be fixed with overhead gage, between the front side of described left side plate and the front side of overhead gage, be fixed with front apron, the bottom of described front apron is movably installed with front door, and the inwall of described overhead gage is provided with a ultraviolet lamp;
Described nucleic acid extraction mechanism comprises a platform support, a vertical guide, a magnetic conductive rod, a magnetizing apparatus, on described platform support, be placed with the magnetic rod sleeve of holding the deep-well plates of reaction reagent and sample and coordinating with magnetic conductive rod, described magnetic conductive rod is arranged in vertical guide and along vertical guide and moves up and down, and described magnetizing apparatus is positioned at the below of magnetic conductive rod and has the communicating pores inserting for magnetic conductive rod;
Described reagent distributor gears comprises that a workplatform, a movable arm, move liquid head, a cross slide way, on described workplatform, be placed with reaction reagent, sample and microwell plate, described movable arm is arranged on cross slide way, this movable arm moves left and right between workplatform and platform support along cross slide way, the described liquid head that moves is arranged on movable arm, and this moves liquid head and moves forward and backward and can on movable arm, vertically move up and down along movable arm.
2. Automatic nucleic acid Testing reaction unit according to claim 1, is characterized in that, the end of described magnetizing apparatus is provided with radiator fan, and this end is connected with guide duct.
3. Automatic nucleic acid Testing reaction unit according to claim 1, it is characterized in that, described overhead gage is divided into the first overhead gage and the second overhead gage, wherein the first overhead gage is positioned at the top of reagent distributor gears, the second overhead gage is positioned at the top of nucleic acid extraction mechanism, and described ultraviolet lamp is arranged on the inwall of the first overhead gage.
4. Automatic nucleic acid Testing reaction unit according to claim 1, it is characterized in that, described front apron is divided into the first front apron and the second front apron, and described rear deflector door is divided into the first rear deflector door, the second rear deflector door and the 3rd rear deflector door, and described front door is divided into the first front door and the second front door.
5. Automatic nucleic acid Testing reaction unit according to claim 2, is characterized in that, described magnetizing apparatus is hollow structure.
6. Automatic nucleic acid Testing reaction unit according to claim 1, is characterized in that, the lower end of described magnetic conductive rod is provided with 96 magnetic conductive heads.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420201952.5U CN203866318U (en) | 2014-04-23 | 2014-04-23 | Full-automatic nucleic acid detect detection and reaction device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420201952.5U CN203866318U (en) | 2014-04-23 | 2014-04-23 | Full-automatic nucleic acid detect detection and reaction device |
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| Publication Number | Publication Date |
|---|---|
| CN203866318U true CN203866318U (en) | 2014-10-08 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420201952.5U Expired - Lifetime CN203866318U (en) | 2014-04-23 | 2014-04-23 | Full-automatic nucleic acid detect detection and reaction device |
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| Country | Link |
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| CN (1) | CN203866318U (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701527A (en) * | 2015-08-11 | 2017-05-24 | 广州康昕瑞基因健康科技有限公司 | Reagent workstation |
| CN111925935A (en) * | 2020-09-08 | 2020-11-13 | 广州市宝创生物技术有限公司 | Sample processing and detecting multifunctional device |
| CN112011450A (en) * | 2020-09-08 | 2020-12-01 | 广州市宝创生物技术有限公司 | One-stop sample detection device |
-
2014
- 2014-04-23 CN CN201420201952.5U patent/CN203866318U/en not_active Expired - Lifetime
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106701527A (en) * | 2015-08-11 | 2017-05-24 | 广州康昕瑞基因健康科技有限公司 | Reagent workstation |
| CN111925935A (en) * | 2020-09-08 | 2020-11-13 | 广州市宝创生物技术有限公司 | Sample processing and detecting multifunctional device |
| CN112011450A (en) * | 2020-09-08 | 2020-12-01 | 广州市宝创生物技术有限公司 | One-stop sample detection device |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CX01 | Expiry of patent term |
Granted publication date: 20141008 |
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| CX01 | Expiry of patent term |