CN203741336U - Biological safety type zymophyte liquid hollow fiber ultrafiltration system - Google Patents
Biological safety type zymophyte liquid hollow fiber ultrafiltration system Download PDFInfo
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- CN203741336U CN203741336U CN201420141206.1U CN201420141206U CN203741336U CN 203741336 U CN203741336 U CN 203741336U CN 201420141206 U CN201420141206 U CN 201420141206U CN 203741336 U CN203741336 U CN 203741336U
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- ultrafiltration
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- hollow fiber
- ultrafiltration tank
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Abstract
The utility model discloses biological safety type zymophyte liquid hollow fiber ultrafiltration system. A fermentation tank, an ultrafiltration tank, an ultrafilter and a disinfection tank are connected through a totally closed pipeline for transportation and ultrafiltration, so that in one aspect, external microbial pollution is prevented, and in the other aspect, a zymophyte liquid is prevented from leaking; as needed, each device is independently cleaned and disinfected or the whole ultrafiltration system is cleaned and disinfected at a time through opening or closing (controlling) all valves. The biological safety type zymophyte liquid hollow fiber ultrafiltration system is simple and practical in structure, simple, convenient and rapid to operate, and low in manufacturing cost; a pump (5) adopts a sanitary centrifugal pump and is provided with a flow sensor; a pressure sensor and a liquid level sensor or a liquid volume sensor are further arranged in the ultrafiltration tank, and the ultrafiltration metering is accurate; the lower openings of an ultrafiltration tank liquid inlet pipe and an ultrafiltration tank return pipe are at 1/3 and 1/5 of the lower part of the ultrafiltration tank, and foam is prevented; the biological safety type zymophyte liquid hollow fiber ultrafiltration system fully utilizes a disinfection device of the fermentation tank, is applicable to ultrafiltration and concentration of a zymophyte liquid sample under the aseptic condition, and replacement of a buffer solution, and also applicable to the degerming and supernatant collection of a secreting type zymophyte liquid.
Description
Technical field
the utility model belongs to biological technical field, is specifically related to biological safe type zymocyte liquid Hollow Fiber Ultrafiltration system.
Background technology
in the culturing process of bacterium, after tank fermentation, need to remove nutrient solution by fermentation, washing bacterium, the concentrated thalline of collecting, routine is that the fermented liquid in fermentor tank is moved in holding tank, the fermented liquid centrifugal supernatant that goes again in holding tank, collect thalline, the thalline of collecting is again with damping fluid washing, filtration, centrifugal, repeatedly carries out; After completing, to the disinfection again of all apparatus, discharge after waste liquid also will carry out disinfection.Whole process operation is loaded down with trivial details, time-consuming, effort.Easily pollute sweathouse, operator is also existed to the risk of infection.
Summary of the invention
the purpose of this utility model is in order to overcome the loaded down with trivial details steps such as the centrifugal collection of zymocyte liquid thalline, thalline washing, guarantee thalline do not leak contaminate environment, operation room and threat operator, and provide, a kind of totally-enclosed pipeline connection is transported, ultrafiltration, collection duct type transmission bacterium liquid, stopping property ultrafiltration bacterium liquid, washing thalline, displacement damping fluid in the ultrafiltration system of one, biological safe type zymocyte liquid Hollow Fiber Ultrafiltration system.
biological safe type zymocyte liquid Hollow Fiber Ultrafiltration system, it comprises: fermentor tank 1, ultrafiltration tank 2, ultra-fine filter 3, disinfection tank 4 and pump 5; Described fermentor tank 1 is provided with the import 14 of calming the anger, fermentor tank drain hole 13; On the cover of described ultrafiltration tank 2, there is ultrafiltration tank liquid-inlet pipe 21, ultrafiltration tank return line 22, described ultrafiltration tank liquid-inlet pipe 21 is provided with ultrafiltration tank opening for feed 212, ultrafiltration tank return line 22 is provided with ultrafiltration tank return line main valve 221, ultrafiltration tank return line 22 is provided with ultrafiltration tank refluxing opening 223 and cleaning disinfection liquid delivery pipe 222, and with the air inlet-outlet pipe 24 of filtration unit, ultrafiltration tank 2 bottoms are provided with ultrafiltration tank drain hole 26 and the ultrafiltration tank drainage port 25 of intercommunication; Described ultra-fine filter 3, is pillar filtering device using hollow fiber ultrafiltration membrane, and it comprises: body and intrinsic hollow fiber ultrafiltration membrane 31, and hollow fiber ultrafiltration membrane 31 upper/lower terminals are established respectively ultra-fine filter liquid collecting leakage fluid dram 311 and ultra-fine filter liquid collecting fluid inlet 312; Body upper/lower terminal is respectively equipped with filtrate relief outlet 321 and ultra-fine filter washing mouth 322; Fermentor tank drain hole 13 and ultrafiltration tank liquid-inlet pipe 21, ultrafiltration tank refluxing opening 223 and ultra-fine filter liquid collecting leakage fluid dram 311, ultrafiltration tank drainage port 25 and pump 5, pump 5 and ultra-fine filter liquid collecting fluid inlet 312, all by pipeline, be connected, filtrate relief outlet 321 and ultra-fine filter washing mouth 322 access in disinfection tank 4 by pipeline;
on the cover of described ultrafiltration tank 2, be also provided with thermometer 23;
on described ultrafiltration tank liquid-inlet pipe 21, be also provided with ultrafiltration tank scavenging solution import 211;
described fermentor tank 1 fermentor tank drain hole 13 is provided with high compressed steam entrance 12;
described pump 5 is sanitary centrifugal pump, and is provided with flow rate sensor;
in described ultrafiltration tank 2, be also provided with pressure transmitter, liquid level or liquid volume sensor,
described ultrafiltration tank liquid-inlet pipe 21 and the end opening of ultrafiltration tank return line 22 under ultrafiltration tank 1 3-1 5 places;
each described interface connecting tube is silicone tube.
the utility model provides biological safe type zymocyte liquid Hollow Fiber Ultrafiltration system, from fermentor tank, ultrafiltration tank, ultra-fine filter to disinfection tank, adopts totally-enclosed pipeline to connect to transport, ultrafiltration; Prevent that on the one hand external microbial from polluting, prevent that on the other hand zymocyte liquid from leaking; Can by opening or closing (control) each valve, to each, install cleaning and sterilizing respectively as required, also can be to the disposable cleaning and sterilizing of whole ultrafiltration system.Quick, low cost of manufacture simple and practical, easy and simple to handle; Pump 5 adopts sanitary centrifugal pump, and is provided with flow rate sensor; In ultrafiltration tank, be also provided with pressure transmitter, liquid level or liquid volume sensor, ultrafiltration accurate measurement; The end opening of ultrafiltration tank liquid-inlet pipe and ultrafiltration tank return line under ultrafiltration tank 1 3-1 5 places, prevent foam; This ultrafiltration system takes full advantage of the sterilizing unit of fermentor tank, applicable to the ultrafiltration of aseptic condition bottom fermentation tank bacterium liquid sample, concentrated, damping fluid displacement; Also can collect for degerming and the supernatant of secretor type zymocyte liquid.
Accompanying drawing explanation
fig. 1 is the schematic diagram of the utility model biological safe type zymocyte liquid Hollow Fiber Ultrafiltration system.
Embodiment
embodiment 1 biological safe type zymocyte liquid Hollow Fiber Ultrafiltration system
referring to accompanying drawing 1, biological safe type zymocyte liquid Hollow Fiber Ultrafiltration system, it comprises: fermentor tank 1, ultrafiltration tank 2, ultra-fine filter 3, disinfection tank 4 and pump 5;
described fermentor tank 1 is provided with the import 14 of calming the anger, fermentor tank drain hole 13;
on the cover of described ultrafiltration tank 2, there is ultrafiltration tank liquid-inlet pipe 21, ultrafiltration tank return line 22, described ultrafiltration tank liquid-inlet pipe 21 is provided with ultrafiltration tank opening for feed 212, ultrafiltration tank return line 22 is provided with ultrafiltration tank return line main valve 221, ultrafiltration tank return line 22 is provided with ultrafiltration tank refluxing opening 223 and cleaning disinfection liquid delivery pipe 222, and with the air inlet-outlet pipe 24 of filtration unit, ultrafiltration tank 2 bottoms are provided with ultrafiltration tank drain hole 26 and the ultrafiltration tank drainage port 25 of intercommunication;
described ultra-fine filter 3, is pillar filtering device using hollow fiber ultrafiltration membrane, and it comprises: body and intrinsic hollow fiber ultrafiltration membrane 31, and hollow fiber ultrafiltration membrane 31 upper/lower terminals are established respectively ultra-fine filter liquid collecting leakage fluid dram 311 and ultra-fine filter liquid collecting fluid inlet 312; Body upper/lower terminal is respectively equipped with filtrate relief outlet 321 and ultra-fine filter washing mouth 322;
fermentor tank drain hole 13 and ultrafiltration tank liquid-inlet pipe 21, ultrafiltration tank refluxing opening 223 and ultra-fine filter liquid collecting leakage fluid dram 311, ultrafiltration tank drainage port 25 and pump 5, pump 5 and ultra-fine filter liquid collecting fluid inlet 312, all by pipeline, be connected, filtrate relief outlet 321 and ultra-fine filter washing mouth 322 access in disinfection tank 4 by pipeline;
on the cover of described ultrafiltration tank 2, be also provided with thermometer 23;
on described ultrafiltration tank liquid-inlet pipe 21, be also provided with ultrafiltration tank scavenging solution import 211;
described fermentor tank 1 fermentor tank drain hole 13 is provided with high compressed steam entrance 12;
described pump 5 is sanitary centrifugal pump, and is provided with flow rate sensor;
in described ultrafiltration tank 2, be also provided with pressure transmitter, liquid level or liquid volume sensor,
described ultrafiltration tank liquid-inlet pipe 21 and the end opening of ultrafiltration tank return line 22 under ultrafiltration tank 1 3-1 5 places;
each described interface connecting tube is silicone tube.
during use:
one, ultrafiltration
1, in fermentor tank 1 fermentation, arrive the degree needing, close valve and the fermentor tank 1 tail gas valve of ultrafiltration tank scavenging solution import 211, ultra-fine filter washing mouth 322, cleaning disinfection liquid delivery pipe 222, ultrafiltration tank drain hole 26, open fermentor tank drain hole 13; Tank inner air pressure moves into ultrafiltration tank 1 by zymocyte liquid, if any residue, and compressed import 14 pressurizations of calming the anger, fermented liquid all moves into ultrafiltration tank 1, closes ultrafiltration tank opening for feed 212; In fermentor tank 1, fill with the decontamination venom that disappears, fermentor tank 1 is carried out disinfection;
2, ejector priming 5, in ultrafiltration tank, fermented liquid enters ultrafiltration tank 2 through ultrafiltration tank drainage port 25, ultra-fine filter liquid collecting leakage fluid dram 311, ultra-fine filter liquid collecting fluid inlet 312, hollow fiber ultrafiltration membrane 31, ultra-fine filter liquid collecting leakage fluid dram 311, ultrafiltration tank refluxing opening 223, ultrafiltration tank return line 22, circulation; The filtrate that hollow fiber ultrafiltration membrane 31 leaches, through filtrate relief outlet 321, enters disinfection tank sterilization; In fermentor tank 1, add lavation buffer solution, open ultrafiltration tank opening for feed 212, fermentor tank 1 adds pressurized air, the interior supplementary lavation buffer solution of ultrafiltration tank 2, or utilize peristaltic pump through ultrafiltration tank scavenging solution import 211, and in ultrafiltration tank 2, pump into lavation buffer solution, continue loop ultrafiltration; After ultrafiltration finishes, open ultra-fine filter washing mouth 322, the filtrate of putting in ultra-fine filter is all put into disinfection tank, opens ultrafiltration tank drain hole 26 and puts into holding tank, closes ultrafiltration tank drain hole 26;
two, cleaning and sterilizing
1, each device difference cleaning and sterilizing
1) close ultra-fine filter washing mouthful 322 valves, open fermentor tank drain hole 13 valves; Through 14 pressurizations of fermentor tank compressed air inlet, cleaning disinfection liquid moves in ultrafiltration tank 2;
or close ultrafiltration tank opening for feed 212, and utilize peristaltic pump through ultrafiltration tank scavenging solution import 211, in ultrafiltration tank 2, pump into cleaning disinfection liquid;
2) ejector priming 5, in ultrafiltration tank, cleaning disinfection liquid enters ultrafiltration tank 2 through ultrafiltration tank drainage port 25, ultra-fine filter liquid collecting leakage fluid dram 311, ultra-fine filter liquid collecting fluid inlet 312, hollow fiber ultrafiltration membrane 31, ultra-fine filter liquid collecting leakage fluid dram 311, ultrafiltration tank refluxing opening 223, ultrafiltration tank return line 22, circulation, cleaning and sterilizing; The part cleaning disinfection liquid that hollow fiber ultrafiltration membrane 31 leaches, through filtrate relief outlet 321, enters disinfection tank 4; Cleaning and sterilizing reached after predetermined time, closed ultrafiltration tank return line main valve 221, opened cleaning disinfection liquid delivery pipe 222; Cleaning disinfection liquid enters disinfection tank or directly discharges from cleaning disinfection liquid delivery pipe 222.
in fermentor tank 1, add cleaning disinfection liquid (changing in advance), or close ultrafiltration tank opening for feed 212, utilize peristaltic pump through ultrafiltration tank scavenging solution import 211, in ultrafiltration tank 2, pump into cleaning disinfection liquid; Repeating step 2).With pure water, 0.5N sodium hydroxide, pure water, clean ultrafiltration system successively, finally with 0.5% formaldehyde, seal system up for safekeeping.
, continuous wash sterilization
1) close ultrafiltration tank return line main valve 221, open cleaning disinfection liquid delivery pipe 222; Open fermentor tank drain hole 13 valves simultaneously;
2) through fermentor tank pressurization, inject cleaning disinfection liquid, ejector priming 5, in ultrafiltration tank, cleaning disinfection liquid is through ultrafiltration tank drainage port 25, ultra-fine filter liquid collecting leakage fluid dram 311, ultra-fine filter liquid collecting fluid inlet 312, hollow fiber ultrafiltration membrane 31, ultra-fine filter liquid collecting leakage fluid dram 311, ultrafiltration tank refluxing opening 223, ultrafiltration tank return line 22, cleaning disinfection liquid delivery pipe 222, and cleaning disinfection liquid enters disinfection tank or directly discharges from cleaning disinfection liquid delivery pipe 222.The part cleaning disinfection liquid that hollow fiber ultrafiltration membrane 31 leaches, through filtrate relief outlet 321, enters disinfection tank 4 or directly discharges; Cleaning and sterilizing reaches predetermined time, and after emptying cleaning disinfection liquid, then change cleaning disinfection liquid and repeat aforesaid operations.With pure water, 0.5N sodium hydroxide, pure water, clean ultrafiltration system successively, finally with 0.5% formaldehyde, seal system up for safekeeping.Also the available logical high compressed steam of high compressed steam entrance 12 by fermentor tank carries out disinfection.
the utility model biological safe type zymocyte liquid Hollow Fiber Ultrafiltration system, from fermentor tank 1, ultrafiltration tank 2, ultra-fine filter 3 to disinfection tank 4, adopts totally-enclosed pipeline to connect to transport, ultrafiltration; Prevent that on the one hand external microbial from polluting, prevent that on the other hand zymocyte liquid from leaking; Can by opening or closing (control) each valve, to each, install cleaning and sterilizing respectively as required, also can be to the disposable cleaning and sterilizing of whole ultrafiltration system.Quick, low cost of manufacture simple and practical, easy and simple to handle; Pump 5 adopts sanitary centrifugal pump, and is provided with flow rate sensor; In ultrafiltration tank 2, be also provided with pressure transmitter, liquid level or liquid volume sensor, ultrafiltration accurate measurement; The end opening of ultrafiltration tank liquid-inlet pipe 21 and ultrafiltration tank return line 22 under ultrafiltration tank 1 3-1 5 places, prevent foam; This ultrafiltration system takes full advantage of the sterilizing unit of fermentor tank, applicable to the ultrafiltration of aseptic condition bottom fermentation tank bacterium liquid sample, concentrated, damping fluid displacement.
Claims (8)
1. biological safe type zymocyte liquid Hollow Fiber Ultrafiltration system, its feature with in, it comprises: fermentor tank [1], ultrafiltration tank [2], ultra-fine filter [3], disinfection tank [4] and pump [5]; Described fermentor tank [1] is provided with the import [14] of calming the anger, fermentor tank drain hole [13]; On the cover of described ultrafiltration tank [2], there is ultrafiltration tank liquid-inlet pipe [21], ultrafiltration tank return line [22], described ultrafiltration tank liquid-inlet pipe [21] is provided with ultrafiltration tank opening for feed [212], ultrafiltration tank return line [22] is provided with ultrafiltration tank return line main valve [221], ultrafiltration tank return line [22] is provided with ultrafiltration tank refluxing opening [223] and cleaning disinfection liquid delivery pipe [222], and with the air inlet-outlet pipe [24] of filtration unit, ultrafiltration tank [2] bottom is provided with ultrafiltration tank drain hole [26] and the ultrafiltration tank drainage port [25] of intercommunication; Described ultra-fine filter [3], for pillar filtering device using hollow fiber ultrafiltration membrane, it comprises: body and intrinsic hollow fiber ultrafiltration membrane [31], and hollow fiber ultrafiltration membrane [31] upper/lower terminal is established respectively ultra-fine filter liquid collecting leakage fluid dram [311] and ultra-fine filter liquid collecting fluid inlet [312]; Body upper/lower terminal is respectively equipped with filtrate relief outlet [321] and ultra-fine filter washing mouthful [322]; Fermentor tank drain hole [13] and ultrafiltration tank liquid-inlet pipe [21], ultrafiltration tank refluxing opening [223] and ultra-fine filter liquid collecting leakage fluid dram [311], ultrafiltration tank drainage port [25] and pump [5], pump [5] and ultra-fine filter liquid collecting fluid inlet [312], all by pipeline, be connected, filtrate relief outlet [321] and ultra-fine filter washing mouthful [322] access in disinfection tank [4] by pipeline.
2. Ju biological safe type zymocyte liquid claimed in claim 1 Hollow Fiber Ultrafiltration system, is characterized in that: on the cover of described ultrafiltration tank [2], be also provided with thermometer [23].
3. Ju biological safe type zymocyte liquid claimed in claim 1 Hollow Fiber Ultrafiltration system, is characterized in that: on described ultrafiltration tank liquid-inlet pipe [21], be also provided with ultrafiltration tank scavenging solution import [211].
4. Ju biological safe type zymocyte liquid claimed in claim 3 Hollow Fiber Ultrafiltration system, is characterized in that: described fermentor tank [1] fermentor tank drain hole [13] is provided with high compressed steam entrance [12].
5. Ju biological safe type zymocyte liquid claimed in claim 4 Hollow Fiber Ultrafiltration system, is characterized in that: described pump [5] is sanitary centrifugal pump, and is provided with flow rate sensor.
6. Ju biological safe type zymocyte liquid claimed in claim 5 Hollow Fiber Ultrafiltration system, is characterized in that: in described ultrafiltration tank [2], be also provided with pressure transmitter, liquid level or liquid volume sensor.
7. Ju biological safe type zymocyte liquid claimed in claim 6 Hollow Fiber Ultrafiltration system, is characterized in that: described ultrafiltration tank liquid-inlet pipe [21] and the end opening of ultrafiltration tank return line [22] under ultrafiltration tank 1 3-1 5 places.
8. Ju biological safe type zymocyte liquid claimed in claim 7 Hollow Fiber Ultrafiltration system, is characterized in that: each described interface connecting tube is silicone tube.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420141206.1U CN203741336U (en) | 2014-03-27 | 2014-03-27 | Biological safety type zymophyte liquid hollow fiber ultrafiltration system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201420141206.1U CN203741336U (en) | 2014-03-27 | 2014-03-27 | Biological safety type zymophyte liquid hollow fiber ultrafiltration system |
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| Publication Number | Publication Date |
|---|---|
| CN203741336U true CN203741336U (en) | 2014-07-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201420141206.1U Expired - Fee Related CN203741336U (en) | 2014-03-27 | 2014-03-27 | Biological safety type zymophyte liquid hollow fiber ultrafiltration system |
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| CN (1) | CN203741336U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107674825A (en) * | 2016-08-02 | 2018-02-09 | 智水科技有限公司 | Microorganism culture concentration filtration system |
-
2014
- 2014-03-27 CN CN201420141206.1U patent/CN203741336U/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107674825A (en) * | 2016-08-02 | 2018-02-09 | 智水科技有限公司 | Microorganism culture concentration filtration system |
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| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20140730 Termination date: 20150327 |
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| EXPY | Termination of patent right or utility model |