CN205443281U - Anaerobic culture medium filters degerming system - Google Patents
Anaerobic culture medium filters degerming system Download PDFInfo
- Publication number
- CN205443281U CN205443281U CN201620126892.4U CN201620126892U CN205443281U CN 205443281 U CN205443281 U CN 205443281U CN 201620126892 U CN201620126892 U CN 201620126892U CN 205443281 U CN205443281 U CN 205443281U
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- China
- Prior art keywords
- valve
- culture medium
- gas circuit
- anaerobic
- filter
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- 239000001963 growth medium Substances 0.000 title claims abstract description 33
- 239000012530 fluid Substances 0.000 claims description 47
- 239000007789 gas Substances 0.000 claims description 37
- 230000001954 sterilising effect Effects 0.000 claims description 26
- 238000004659 sterilization and disinfection Methods 0.000 claims description 25
- 238000001914 filtration Methods 0.000 claims description 18
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 9
- 239000001301 oxygen Substances 0.000 claims description 9
- 229910052760 oxygen Inorganic materials 0.000 claims description 9
- 239000007788 liquid Substances 0.000 abstract description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 16
- 229910052757 nitrogen Inorganic materials 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 238000005516 engineering process Methods 0.000 description 4
- 230000005587 bubbling Effects 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000002955 isolation Methods 0.000 description 2
- 239000002609 medium Substances 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 241000209094 Oryza Species 0.000 description 1
- 229910000831 Steel Inorganic materials 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 238000006392 deoxygenation reaction Methods 0.000 description 1
- 238000007599 discharging Methods 0.000 description 1
- 238000006073 displacement reaction Methods 0.000 description 1
- 235000003642 hunger Nutrition 0.000 description 1
- 229940057995 liquid paraffin Drugs 0.000 description 1
- 239000012188 paraffin wax Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000037351 starvation Effects 0.000 description 1
- 239000010959 steel Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 210000003437 trachea Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to an anaerobic culture medium filters degerming system. Including anaerobic air supply jar, first liquid storage pot, filter, second liquid storage pot and anaerobic air pocket, including the gas circuit, its one end is connected to anaerobic air supply jar, the other end passes through valve B1 and B3 feeds through to the top of first liquid storage pot, including the liquid way, its one end pass through valve A2 and A3 intercommunication extremely bottom, the other end of first liquid storage pot pass through valve K2 and K3 feeds through to the entry of filter, including valve A1, its entry linkage is between A2 and A3, including the gas circuit, its one end pass through valve B2 connect between B1 and the B3, the other end passes through valve K1 and connects between K2 and K3, including the liquid way, export, the other end that its one end was connected to filter 7 are connected to the bottom of second liquid storage pot through valve C2 and C3, including valve C1, its entry linkage between C2 and C3, the exit linkage has the gas circuit, still include the gas circuit, its one end is connected to the anaerobic air pocket through top, the other end that valve D1 was connected to the second liquid storage pot.
Description
Technical field
This utility model belongs to anaerobe and cultivates facility technology field, particularly relates to a kind of anaerobic culture medium filtration sterilization system.
Background technology
Anaerobic culture medium requires anaerobic, so being desirable that isolation air in preparation sterilization process, typically using deoxygenation the mode with paraffin sealing liquid face of boiling when small lot medium sterilization, being then difficult to operation in high volume during medium sterilization.
The degerming method of existing culture medium generally uses moist heat sterilization, due to the particularity of anaerobic culture medium, it is desirable to anaerobic culture medium keep anaerobic, with guarantee anaerobe can in culture medium normal growth.The containers such as moist heat sterilization is usually and carries out fluid-tight with liquid paraffin etc., and this method typically carries out laboratory use in laboratory small lot, uses test tube, triangular flask.The sterilizing methods of the anaerobic culture medium currently used, by batch quantitative limitation, when use in high volume, workload is very big, and time-consuming is longer.
Utility model content
This utility model provides a kind of anaerobic culture medium filtration sterilization system for solving technical problem present in known technology, is applied to batch anaerobic culture medium filtration sterilization, the starvation impact on anaerobic culture medium, keeps culture medium anaerobic state.
This utility model is adopted the technical scheme that by solving technical problem present in known technology: one anaerobic culture medium filtration sterilization system includes no oxygen carrying shield, the first fluid reservoir, filter, the second fluid reservoir and anaerobic airbag；Also including the first gas circuit, its one end is connected to the outlet of no oxygen carrying shield, and the other end is communicated to the inner chamber of the first fluid reservoir by the valve B1 that is connected in series and valve B3 and end is positioned at the top of this inner chamber；Also including the first fluid path, its one end is communicated to the inner chamber of the first fluid reservoir by the valve A2 that is connected in series and valve A3 and end is positioned at the bottom of this inner chamber, and the other end of the first fluid path is communicated to the entrance of filter by the valve K2 being connected in series and valve K3；Also including valve A1, its entrance is connected on the pipeline between valve A2 and valve A3；Also including the second gas circuit, its one end is connected on the pipeline between valve B1 and valve B3 by valve B2, the other end is connected on the pipeline between valve K2 and valve K3 by valve K1；Also include the second fluid path, its one end is connected to the outlet of filter, the other end and is connected to the inner chamber of the second fluid reservoir by the valve C2 that is connected in series and valve C3 and end is positioned at the bottom of this inner chamber, also including valve C1, its entrance is connected on the pipeline between valve C2 and valve C3, export connection the 3rd gas circuit；Also including the 4th gas circuit, its one end is connected to the inner chamber of the second fluid reservoir by valve D1 and end is positioned at the top of this inner chamber, and the other end of the 4th gas circuit is connected to anaerobic airbag.
Advantage of the present utility model and good effect be: this utility model provides the anaerobic culture medium filtration sterilization system of a kind of reasonable in design, compared with existing filtration sterilization system and filtration sterilization mode, the technical program has the advantage that, (1) full-closed system, contacts oxygen during can being separated out filtration sterilization completely；(2) integrity mensuration' of filter membrane uses oxygenless gas, and in filter process, in film, gas is difficult to discharge, very big on culture medium impact, uses oxygenless gas then can avoid impact；(3) anaerobic culture medium can be carried out in high volume degerming；(4), in the culture medium after filtration sterilization is saved in the sterile closed fluid reservoir of anaerobic, keeps the aseptic condition of anaerobic culture medium, facilitate the later stage to use；(5) fluid reservoir is total enclosing, keeps anaerobic during the course, even if can not filter immediately, it is also possible to preserves a period of time, and makes culture medium not affected by oxygen；(6) tank body and connection pipe fitting use stainless steel, can carry out high pressure steam sterilization, anticorrosion；(7) connect the trachea used and liquid pipe can use flexible pipe, convenient connection.
Preferably: in the first gas circuit, be provided with air relief valve.
Preferably: in the first fluid path, be provided with Pressure gauge.
Preferably: described valve A1, valve A2, valve A3, valve B1, valve B2, valve B3, valve C1, valve C2, valve C3, valve D1, valve K1, valve K2 and valve K3 are separate control valves door.
Preferably: described filter is chosen for plate frame filter.
Accompanying drawing explanation
Fig. 1 is structured flowchart of the present utility model.
In figure: 1, the first fluid reservoir；2, air relief valve；3, the first gas circuit；4, the first fluid path；5, Pressure gauge；6, the second gas circuit；7, filter；8, the second fluid path；9, the 3rd gas circuit；10, the second fluid reservoir；11, the 4th gas circuit；12, airbag；A1, A2, A3, B1, B2, B3, C1, C2, C3, D1, K1, K2, K3 are valve.
Detailed description of the invention
For summary of the invention of the present utility model, feature and effect can be further appreciated that, hereby lift following example and describe in detail as follows:
Refer to Fig. 1, anaerobic culture medium filtration sterilization system of the present utility model includes no oxygen carrying shield, the first fluid reservoir 1, filter the 7, second fluid reservoir 10 and anaerobic airbag 12, wherein stores in no oxygen carrying shield and stores a small amount of aseptic anaerobic source of the gas in aseptic high pressure anaerobic source of the gas, anaerobic airbag 12.Specifically, anaerobic source of the gas can be chosen for nitrogen, and the first fluid reservoir 1 and the second fluid reservoir 10 are chosen for rustless steel vapor tight tank.
Also including the first gas circuit 3, its one end is connected to the outlet of no oxygen carrying shield, and the other end is communicated to the inner chamber of the first fluid reservoir 1 by the valve B1 that is connected in series and valve B3 and end is positioned at the top of this inner chamber.
Also include the first fluid path 4, its one end is communicated to the inner chamber of the first fluid reservoir 1 by the valve A2 that is connected in series and valve A3 and end is positioned at the bottom of this inner chamber, and the other end of the first fluid path 4 is communicated to the entrance of filter 7 by the valve K2 being connected in series and valve K3.
Also including valve A1, its entrance is connected on the pipeline between valve A2 and valve A3.
Also including the second gas circuit 6, its one end is connected on the pipeline between valve B1 and valve B3 by valve B2, the other end is connected on the pipeline between valve K2 and valve K3 by valve K1.
Also include the second fluid path 8, its one end is connected to the outlet of filter 7, the other end and is connected to the inner chamber of the second fluid reservoir 10 by the valve C2 that is connected in series and valve C3 and end is positioned at the bottom of this inner chamber, also including valve C1, its entrance is connected on the pipeline between valve C2 and valve C3, export connection the 3rd gas circuit 9.
Also including the 4th gas circuit 11, its one end is connected to the inner chamber of the second fluid reservoir 10 by valve D1 and end is positioned at the top of this inner chamber, and the other end of the 4th gas circuit 11 is connected to anaerobic airbag 12.
In the present embodiment, in order to high-pressure air source is decompressed to convenient pressure, the first gas circuit 3 is provided with air relief valve 2.
In the present embodiment, in order to the pressure (filter pressure) in pipeline is monitored, the first fluid path 4 is provided with Pressure gauge 5.
In the present embodiment, valve A1, valve A2, valve A3, valve B1, valve B2, valve B3, valve C1, valve C2, valve C3, valve D1, valve K1, valve K2 and valve K3 are separate control valves door, coordinated the purpose reaching different by switch, the Automated condtrol coordinating automatic control system to realize whole anaerobic culture medium filtration sterilization system runs.
In the present embodiment, filter 7 is chosen for plate frame filter.
Work process:
(1) pipeline evacuation, before using, the first fluid reservoir 1 and the second fluid reservoir 10 are replaced into nitrogen (or other oxygenless gas), can be by two kinds of method substitution gas: one is to be filled with nitrogen from bottom conduit, discharging gas from upper pipeline, after certain time, air in tank is replaced by nitrogen completely simultaneously；A kind of is that the air first extracted out in fluid reservoir forms negative pressure, is re-filled with nitrogen and forms malleation, 3-4 time repeatedly, completes gas displacement.
(2) using vacuum pump that the anaerobic culture medium prepared is transferred to the first fluid reservoir 1, filter the 7, second fluid reservoir 10 and associated pipe carry out sterilization treatment.After carrying out the building of system as illustrated, first valve A3, B3 and K3 close, valve A1, A2, B1, B2, K1 and K2 open, sterile nitrogen enters first gas circuit the 3, second gas circuit 6 by air relief valve 2 and the first fluid path 4 is finally discharged by A1, air emptying in pipeline after certain time, makes pipeline form oxygen-free environment.
(3) valve B1, B3, A3, A2, K2, K3, C2 and C1 are opened, other valve closing, bubbling point water tumbler is set in the end of the 3rd gas circuit 9, carry out bubbling point test, the effectiveness of authenticated filter its filter membrane of sterilization system.
(4) second fluid reservoirs 10 connect anaerobic airbag 12, store a small amount of sterile nitrogen.
(5) filter pressure is controlled by air relief valve 2, Pressure gauge 5 after starting to filter.Filtration wild Oryza species is opened valve C3 and D1 immediately when valve C1 discharges and is simultaneously closed off valve C1, it is ensured that air a small amount of in gas circuit 8 will not enter the second fluid reservoir 10.
(6) close valve C3 and D1 after having filtered, then anaerobic culture medium has filtered, and is in isolation air condition.
(7) when using culture medium, opening valve D1, along with the minimizing of culture medium in tank, oxygenless gas enters in the second fluid reservoir 10, and the sterile nitrogen of connection can ensure that in use anaerobic culture medium will not ingress of air.
Above content is to combine the detailed description that this utility model is done by detailed description of the invention; its object is to make those skilled in the art it will be appreciated that content of the present utility model implementing according to this, the simple deduction or replace done according to the spirit disclosed in this utility model are still in protection domain of the present utility model.
Claims (5)
1. an anaerobic culture medium filtration sterilization system, is characterized in that: include no oxygen carrying shield, the first fluid reservoir (1), filter (7), the second fluid reservoir (10) and anaerobic airbag (12)；Also including the first gas circuit (3), its one end is connected to the outlet of no oxygen carrying shield, and the other end is communicated to the inner chamber of the first fluid reservoir (1) by the valve B1 that is connected in series and valve B3 and end is positioned at the top of this inner chamber；Also include the first fluid path (4), its one end is communicated to the inner chamber of the first fluid reservoir (1) by the valve A2 that is connected in series and valve A3 and end is positioned at the bottom of this inner chamber, and the other end of the first fluid path (4) is communicated to the entrance of filter (7) by the valve K2 being connected in series and valve K3；Also including valve A1, its entrance is connected on the pipeline between valve A2 and valve A3；Also including the second gas circuit (6), its one end is connected on the pipeline between valve B1 and valve B3 by valve B2, the other end is connected on the pipeline between valve K2 and valve K3 by valve K1；Also include the second fluid path (8), its one end is connected to the outlet of filter (7), the other end and is connected to the inner chamber of the second fluid reservoir (10) by the valve C2 that is connected in series and valve C3 and end is positioned at the bottom of this inner chamber, also including valve C1, its entrance is connected on the pipeline between valve C2 and valve C3, export connection the 3rd gas circuit (9)；Also including the 4th gas circuit (11), its one end is connected to the inner chamber of the second fluid reservoir (10) by valve D1 and end is positioned at the top of this inner chamber, and the other end of the 4th gas circuit (11) is connected to anaerobic airbag (12).
2. anaerobic culture medium filtration sterilization system as claimed in claim 1, is characterized in that: be provided with air relief valve (2) in the first gas circuit (3).
3. anaerobic culture medium filtration sterilization system as claimed in claim 1, is characterized in that: be provided with Pressure gauge (5) in the first fluid path (4).
4. anaerobic culture medium filtration sterilization system as claimed in claim 1, is characterized in that: described valve A1, valve A2, valve A3, valve B1, valve B2, valve B3, valve C1, valve C2, valve C3, valve D1, valve K1, valve K2 and valve K3 are separate control valves door.
5. anaerobic culture medium filtration sterilization system as claimed in claim 1, is characterized in that: described filter (7) is chosen for plate frame filter.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620126892.4U CN205443281U (en) | 2016-02-18 | 2016-02-18 | Anaerobic culture medium filters degerming system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201620126892.4U CN205443281U (en) | 2016-02-18 | 2016-02-18 | Anaerobic culture medium filters degerming system |
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| Publication Number | Publication Date |
|---|---|
| CN205443281U true CN205443281U (en) | 2016-08-10 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201620126892.4U Expired - Fee Related CN205443281U (en) | 2016-02-18 | 2016-02-18 | Anaerobic culture medium filters degerming system |
Country Status (1)
| Country | Link |
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| CN (1) | CN205443281U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107175242A (en) * | 2017-07-07 | 2017-09-19 | 湖南大学 | Anaerobic culturel bottle inflation vacuumizes purging system |
| CN108949517A (en) * | 2017-05-19 | 2018-12-07 | 上海吉态来生物技术有限公司 | A kind of the liquid fermentation tank classification inoculation apparatus and inoculation method of obligate anaerobe |
-
2016
- 2016-02-18 CN CN201620126892.4U patent/CN205443281U/en not_active Expired - Fee Related
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108949517A (en) * | 2017-05-19 | 2018-12-07 | 上海吉态来生物技术有限公司 | A kind of the liquid fermentation tank classification inoculation apparatus and inoculation method of obligate anaerobe |
| CN107175242A (en) * | 2017-07-07 | 2017-09-19 | 湖南大学 | Anaerobic culturel bottle inflation vacuumizes purging system |
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| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20160810 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |