CN107674825A - Microorganism culture concentration filtration system - Google Patents
Microorganism culture concentration filtration system Download PDFInfo
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- CN107674825A CN107674825A CN201710641531.2A CN201710641531A CN107674825A CN 107674825 A CN107674825 A CN 107674825A CN 201710641531 A CN201710641531 A CN 201710641531A CN 107674825 A CN107674825 A CN 107674825A
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- 238000001914 filtration Methods 0.000 title claims abstract description 169
- 244000005700 microbiome Species 0.000 title claims abstract description 157
- 239000007788 liquid Substances 0.000 claims abstract description 107
- 239000012141 concentrate Substances 0.000 claims abstract description 56
- 230000008719 thickening Effects 0.000 claims description 112
- 239000000463 material Substances 0.000 claims description 71
- 238000004140 cleaning Methods 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 18
- 238000012544 monitoring process Methods 0.000 claims description 15
- 230000008569 process Effects 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000012528 membrane Substances 0.000 claims description 11
- 238000003860 storage Methods 0.000 claims description 8
- 239000012510 hollow fiber Substances 0.000 claims description 6
- 239000013043 chemical agent Substances 0.000 claims description 5
- 239000012530 fluid Substances 0.000 claims description 5
- 230000000903 blocking effect Effects 0.000 claims description 3
- 239000002657 fibrous material Substances 0.000 claims description 3
- 230000007423 decrease Effects 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract description 2
- 239000011550 stock solution Substances 0.000 abstract 6
- 238000000855 fermentation Methods 0.000 description 8
- 230000004151 fermentation Effects 0.000 description 8
- 241000195493 Cryptophyta Species 0.000 description 6
- 238000010586 diagram Methods 0.000 description 6
- 235000015097 nutrients Nutrition 0.000 description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 230000009471 action Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000007789 gas Substances 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 239000001569 carbon dioxide Substances 0.000 description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000002045 lasting effect Effects 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 238000005096 rolling process Methods 0.000 description 2
- 241000233866 Fungi Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000005056 cell body Anatomy 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000009630 liquid culture Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012806 monitoring device Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000010563 solid-state fermentation Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 238000010408 sweeping Methods 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/10—Separation or concentration of fermentation products
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/02—Membranes; Filters
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
A microorganism culture concentration and filtration system comprises a stock solution culture unit, a concentration relay unit and a concentration and filtration unit, wherein the stock solution culture unit is a container for culturing microorganisms, the stock solution culture unit is provided with a stock solution of the microorganisms, the stock solution with the microorganisms can enter the concentration relay unit through a pipeline and then is conveyed into the concentration and filtration unit through a pipeline, and the purpose of concentrating the cultured microorganisms can be achieved because the concentration and filtration unit can filter the stock solution with the microorganisms into a concentrate with the microorganisms and discharge the liquid without the microorganisms in the concentration and filtration unit.
Description
Technical field
The present invention relates to a kind of microculture thickening filtration system, and more particularly to one kind can be with concentrate microbial nutrient solution
Or produce the microculture thickening filtration system without microbe filter liquid.
Background technology
Microbial fermentation includes two kinds of liquid state fermentation and solid state fermentation, and the fermented quality of wherein liquid state fermentation is relatively stable,
It and fermenting speed is fast, can largely produce in a short time, generally can be divided into a cultivation, Fed batch fementation method and continuous training
The method of supporting, wherein a cultivation is once to add all raw materials and microorganism in culture bucket to cultivate, after the completion of culture, then
The classical culture protocols that content is taken out totally.
And Fed batch fementation method refers to, in microorganism different growing stage, intermittently or serially supplement fresh cultured in batches
MATERIALS METHODS.The raw material mended can be complete feed or nitrogen source, carbon source etc., it is therefore an objective to when extending the synthesis of metabolite
Between quality etc. to reach bigger volume production or control specific products.
And continuous culture method refers to while fermenter adds new nutrient solution, the fermentation side of original nutrient solution is also harvested
Method.Its advantage is that utilization rate of equipment and installations is high, product quality is stable, is easy to automatically control, shortcoming is easy pollution microbes.Generally only
It is adapted to the surging microorganism for having very big adaptability to environment, such as acetic acid bacteria.
And microorganism, in addition to bacterium mentioned above, some algae fall within microorganism, because algae is self-operation
Biology, carbon dioxide are the carbon source of assisting growth, so more efficient culture systems, its algae utilizes the efficiency of carbon dioxide
Also it is higher.Algae culturing system is broadly divided into two kinds of open system and closed system, the open culture systems of algae both known
Mainly there is circular culture pond（Circular pond）And racetrack culture pond（Race-way pond）Two kinds.
Due to more liquid state fermentations that microorganism is carried out using Automatic Control fermentation tank industrial at present, its device includes
The control monitoring device and fermentation tank cell body of integrally formed fermentation tank, in order to avoid the pollution and use in the external world
On convenience；But have a greatest problem is how to separate nutrient solution with microorganism, and microorganism is gradually purified, be exactly in addition
Past, general more methods using centrifugation were handled, but the amount of solution that this processing method can be handled is limited, and efficiency
It is not high.
Therefore, in order to overcome above mentioned problem, if the stoste with microorganism can be carried out by a thickening filtration unit
Filter and discharge the liquid without microorganism, then can reach simultaneously separates the microorganism of culture and be concentrated, together
When produce the purpose of sterile nutrient solution, so should be a best solution.
The content of the invention
A kind of microculture thickening filtration system, including：One stoste culture unit, for one cultivating the appearance of microorganism
Device, the stoste culture unit have the stoste of microorganism；One concentration relay unit, passes through at least one pipeline and the stoste culture
Unit is connected, and the stoste with microorganism can enter the concentration relay unit by the pipeline in the stoste culture unit
In；One thickening filtration unit, it is connected by least one pipeline with the concentration relay unit, and is had in the concentration relay unit
The stoste for having microorganism can be entered in the thickening filtration unit by the pipeline, and the thickening filtration unit will not be to can will
Liquid with microorganism is filtered and discharged, to retain the concentrate with higher concentration microorganism.
In particular, the thickening filtration unit at least declines with a suction type millipore filter and one with the negative pressure
The discharge opeing module of hole filter connection, the suction type millipore filter are connected by least one pipeline with the concentration relay unit
Connect, and the stoste with microorganism can be entered in the suction type millipore filter by the pipeline in the concentration relay unit,
There is at least one negative pressure type micro-hole filter material in the suction type millipore filter, and there should be the stoste of microorganism negative by this
During pressure type micropore filter material, it can should be blocked with the concentrate of microorganism by the negative pressure type micro-hole filter material, and the discharge opeing module then will
The discharge of the liquid without microorganism that can not blocked by the negative pressure type micro-hole filter material.
In particular, the suction type millipore filter can make have micro- life by what the negative pressure type micro-hole filter material blocked
The stoste of thing is back in the concentration relay unit, and repeats to flow back and be circulated throughout to filter the liquid without microorganism, and energy
It is enough to retain the concentrate with higher concentration microorganism in the concentration relay unit and the suction type millipore filter.
In particular, the stoste with microorganism inside the concentration relay unit continues into the negative pressure type micro-hole
Filter carries out excluding the liquid without microorganism, while retaining in the suction type millipore filter has higher concentration micro-
The concentrate of biology.
In particular, the microculture thickening filtration system, in addition at least one take liquid unit and at least one
Individual collector unit, wherein, this takes liquid unit to have retaining in the concentration relay unit and the suction type millipore filter
The concentrate for having higher concentration microorganism takes out and deposited to the collector unit, and the discharge opeing module can not also will have micro- life
The liquid discharge storage of thing is into another collector unit.
In particular, the negative pressure type micro-hole filter material can be hollow fiber form filter membrane, the rolling of hydrophilic film
Filter membrane, plate-type filtering film, tubular filter film, disc filter film or other any micropore filter material institutes for stopping microorganism
Composition.
In particular, also have in the thickening filtration unit and sweep stream cleaning module, can be with gas jet, liquid
Wash away, chemical agent brews, heat or ultrasonic vibrates to be cleaned to negative pressure type micro-hole filter material, to maintain the negative pressure
The cleaning of type micro-hole surface of filter medium and normal filtering function, to avoid microorganism in filter process from blocking the negative pressure type micro-hole
The micropore of surface of filter medium.
In particular, the microculture thickening filtration system also includes one and the concentration relay unit and the concentration
The control unit that filter element is connected, to the management and control concentration relay unit and the filtering operation shape of the thickening filtration unit
State.
In particular, also there is micropore obstruction monitoring modular, to monitor by the negative pressure in described control unit
The pressure and flow of the stoste with microorganism of type micro-hole filter material, to judge micropore degree of congestion.
In particular, also there is a filtering filtering velocity adjusting module, to monitor with microorganism in described control unit
Concentrate by the filter pressure during negative pressure type micro-hole filter material, filtering when flow velocity or concentrate concentration, with as
The reference frame of adjustment filtering filtering velocity.
In particular, also there is a liquid level protection module, to monitor the concentration relay unit in described control unit
And the liquid height of water level inside the suction type millipore filter, and the liquid height of water level to can interpolate that the concentration relay
Unit whether to continue convey the stoste containing microorganism enter the suction type millipore filter in filtered out without micro- life
The processing of the liquid of thing.
In particular, also there is a temperature control module, to monitor and control concentration relaying single in described control unit
Fluid temperature inside member and the suction type millipore filter.
In particular, also there is a defoaming module, to eliminate the concentration relay unit and be somebody's turn to do in described control unit
Liquid inside suction type millipore filter conveys or swept the bubble formed in stream process because of liquid.
In particular, also there is an automatic cleaning module in described control unit, in the concentration relay unit and
In the state of the thickening filtration unit is not operated, it can automatically clean inside the concentration relay unit and the negative pressure declines
Hole filter interior.
In particular, also there is a leakproof monitoring module in described control unit, arranged to monitor the discharge opeing module
Go out the state of liquid, to avoid this from having the concentrate of microorganism can not be completely by the negative pressure type micro-hole because of the breakage of micropore filter material
Filter material blocks, and causes the discharge opeing module row to go out the liquid with microorganism.
In particular, the thickening filtration unit includes a positive-pressure type millipore filter and one and the malleation type micro-hole mistake
The discharge opeing module of filter connection, the positive-pressure type millipore filter are connected by least one pipeline with the concentration relay unit,
And there is a pressure to apply module and at least one malleation type micro-hole filter material in the positive-pressure type millipore filter, concentration relaying is single
The stoste with microorganism can apply module with the pressure of the positive-pressure type millipore filter by the pipeline and be connected in first, so that
When stoste with microorganism is under pressure that driving is lower pass through the malleation type micro-hole filter material, concentrate meeting quilt that should be with microorganism
The malleation type micro-hole filter material blocks, and the discharge opeing module then by the malleation type micro-hole filter material do not block without microorganism
Liquid is discharged.
In particular, the positive-pressure type millipore filter can make have micro- life by what the malleation type micro-hole filter material blocked
The stoste of thing is back in the concentration relay unit, and repeats to flow back and be circulated throughout to filter the liquid without microorganism, then can
It is enough to retain the concentrate with higher concentration microorganism in the concentration relay unit.
In particular, the stoste with microorganism inside the concentration relay unit continues into the malleation type micro-hole
Filter carries out excluding the liquid without microorganism, then can retain in the positive-pressure type millipore filter has higher concentration
The concentrate of microorganism.
In particular, the microculture thickening filtration system also includes at least one liquid unit and at least one of taking
Collector unit, wherein this take liquid unit can will in the concentration relay unit and the suction type millipore filter retain have it is higher
The concentrate of concentration microorganism takes out and deposited to the collector unit, and the discharge opeing module can also be by the liquid without microorganism
Body discharge storage is into another collector unit.
In particular, the malleation type micro-hole filter material can be hollow fiber form filter membrane, the rolling of hydrophilic film
Filter membrane, plate-type filtering film, tubular filter film, disc filter film or fibrous material filter membrane are formed.
In particular, also have in the thickening filtration unit and sweep stream cleaning module, can be with gas jet, liquid
Wash away, chemical agent brews, heat or ultrasonic vibrates to be cleaned to positive-pressure type micropore filter material, to maintain the malleation
The cleaning of type micro-hole surface of filter medium and normal filtering function, to avoid microorganism in filter process from blocking the malleation type micro-hole
The micropore of surface of filter medium.
In particular, the microculture thickening filtration system also includes one and the concentration relay unit and the concentration
The control unit that filter element is connected, to the management and control concentration relay unit and the filtering operation shape of the thickening filtration unit
State.
In particular, also there is micropore obstruction monitoring modular, to monitor by the malleation in described control unit
The pressure and flow of the stoste with microorganism of type micro-hole filter material, to judge micropore degree of congestion.
In particular, also there is a filtering filtering velocity adjusting module, to monitor with microorganism in described control unit
Concentrate by the filter pressure during malleation type micro-hole filter material, filtering when flow velocity or concentrate concentration, with as
The reference frame of adjustment filtering filtering velocity.
In particular, also there is a liquid level protection module, to monitor the concentration relay unit in described control unit
Internal liquid height of water level, and the liquid height of water level is can interpolate that it is micro- whether the concentration relay unit will continue to convey
The stoste of biology enters in the positive-pressure type millipore filter processing for being filtered out the liquid without microorganism.
In particular, also there is a temperature control module, to monitor and control concentration relaying single in described control unit
Fluid temperature inside member.
In particular, also there is a defoaming module, to eliminate inside the concentration relay unit in described control unit
Liquid convey or sweep bubble formed in stream process because of liquid.
In particular, also there is an automatic cleaning module in described control unit, in the concentration relay unit or
In the state of thickening filtration unit is not operated, it can clean automatically in the concentration relay unit or thickening filtration unit
Portion.
In particular, also there is a leakproof monitoring module in described control unit, arranged to monitor the discharge opeing module
Go out the state of liquid, to avoid this that there is the concentrate of microorganism can not be blocked completely by the malleation type micro-hole filter material, and lead
The discharge opeing module row is caused to go out the liquid with microorganism.
Brief description of the drawings
Fig. 1 is the overall architecture schematic diagram of the microculture thickening filtration system of the present invention.
Fig. 2 is the control unit inside structure schematic diagram of the microculture thickening filtration system of the present invention.
Fig. 3 A are the first implementation framework signal of the thickening filtration unit of the microculture thickening filtration system of the present invention
Figure.
Fig. 3 B are the concentrated of the first implementation of the thickening filtration unit of the microculture thickening filtration system of the present invention
Filter process schematic.
Fig. 3 C are the concentrated of the first implementation of the thickening filtration unit of the microculture thickening filtration system of the present invention
Schematic diagram is completed in filter.
Fig. 4 A are the second implementation framework signal of the thickening filtration unit of the microculture thickening filtration system of the present invention
Figure.
Fig. 4 B are the concentrated of the second implementation of the thickening filtration unit of the microculture thickening filtration system of the present invention
Filter process schematic.
Fig. 4 C are the concentrated of the second implementation of the thickening filtration unit of the microculture thickening filtration system of the present invention
Schematic diagram is completed in filter.
Description of reference numerals
1- microculture thickening filtration systems
11- stoste culture units
12- pipelines
13- concentrates relay unit
14- pipelines
15- thickening filtration units
151- suction type millipore filters
1511- negative pressure type micro-hole filter materials
15111- has the concentrate of microorganism
15112- does not have the liquid of microorganism
152- discharge opeing modules
153- sweeps stream cleaning module
154- positive-pressure type millipore filters
1541- pressure applies module
1542- malleation type micro-hole filter materials
15421- has the concentrate of microorganism
15422- does not have the liquid of microorganism
16- pipelines
17- pipelines
18- control units
181- micropores block monitoring modular
182- filters filtering velocity adjusting module
183- liquid level protection modules
184- temperature control modules
185- defoams module
The automatic cleaning modules of 186-
187- leakproof monitoring modules
19- takes liquid unit
20- collector units.
Embodiment
Other technologies content, feature and effect of the present invention are related to, in the preferred embodiment of following cooperation refer to the attached drawing
In detailed description, can clearly it present.
Fig. 1 and Fig. 2 is referred to, is microculture thickening filtration system overall architecture schematic diagram of the present invention and control unit
Inside structure schematic diagram, as shown in FIG., the microculture thickening filtration system 1 comprise at least a stoste culture unit 11,
One concentration relay unit 13, a thickening filtration unit 15, a control unit 18, one take the collection module 20 of liquid module 19 and one, and
The stoste culture unit 11, concentration relay unit 13, thickening filtration unit 15, between control unit 18 by the pipeline 12,13,
14,16,17 carry out liquid conveying.
Wherein, the stoste culture unit 11 be one to cultivate the container of microorganism, and the stoste culture unit have it is micro-
The stoste of biology；Wherein the concentration relay unit 13 is connected by the pipeline 12 with the stoste culture unit 11, so that the original
The stoste with microorganism can then be entered in the concentration relay unit 13 by the pipeline 12 in liquid culture unit 11.
Wherein, the thickening filtration unit 15 is then to be connected by the pipeline 14 with the concentration relay unit 13, so that should
Concentrating the stoste with microorganism in relay unit 13 can be entered in the thickening filtration unit 15 by the pipeline 14, due to this
The inside of thickening filtration unit 15 has at least one set of filter material, therefore the thickening filtration unit 15 then can be by without microorganism
Liquid is filtered and discharged by the pipeline 17, and retains the concentrate with higher concentration microorganism, and has the micro- life of higher concentration
The concentrate of thing can drain back to the concentration relay unit 13 by the pipeline 16, and repeat to filter with the program of backflow by energy
Enough make the microorganism concn more and more higher contained by the stoste in the concentration relay unit 13.
And the control unit 18 is to the management and control concentration relay unit 13 and the filtering operation shape of the thickening filtration unit 15
State, there is in the control unit 18 micropore obstruction monitoring modular 181, one to filter filtering velocity adjusting module 182, liquid level protection mould
Block 183, a temperature control module 184, one defoaming module 185, an automatic leakproof monitoring module 187 of cleaning module 186 and one.
Wherein, micropore obstruction monitoring modular 181 passes through the thickening filtration unit to monitor the stoste with microorganism
Pressure and flow during filter material in 15, to judge micropore degree of congestion, and the filtering filtering velocity adjusting module 182 is then to supervise
Measuring tool have the concentrate of microorganism by flow velocity when filter pressure during filter material in the thickening filtration unit 15, filtering or
The concentration of concentrate, to filter the reference frame of filtering velocity as adjustment.
And the liquid level protection module 183 is then to monitor liquid height of water level, and the liquid height of water level can interpolate that
Concentration relay unit 13 whether to continue conveying tool microorganism stoste enter the thickening filtration unit 15 in filtered out not
The processing of liquid with microorganism；The liquid level protection module 183 can also judge the liquid level water level in thickening filtration unit 15,
To protect micropore filter material not cause to damage or determine the stopping action of discharge concentrate because liquid water level is too low.
And the temperature control module 184 is mainly monitoring and control in the concentration relay unit 13 and thickening filtration unit 15
Fluid temperature；The other defoaming module 185 is then to eliminate liquid inside the concentration relay unit 13 because liquid conveyed
Because liquid conveys or cleans and sweeps bubble formed in stream process in bubble and the thickening filtration unit 15 formed in journey.
And the automatic cleaning module 185 in the concentration relay unit 13 and the thickening filtration unit 15 not transported
In the state of work, it can clean automatically inside the concentration relay unit 13 and inside the filter material of the thickening filtration unit 15, container
And pipeline.In addition to avoid this that there is the concentrate of microorganism can not be blocked completely by the filter material inside the thickening filtration unit 15
Resistance, and cause the discharge that there is the liquid of microorganism, it is therefore necessary to the institute is monitored by the leakproof monitoring module 187 and discharged
The state of liquid, if turbidity is too high, then judge the filter material inside the thickening filtration unit 15 may have damaged leak or its
The problem of he causes filtering to fail.
And the thickening filtration unit 15 can have two kinds of forms, the first is then suction type, and second is positive-pressure type,
It is then suction type that the first of the present invention, which is implemented, and as shown in Fig. 3 A and Fig. 3 B, the inside of thickening filtration unit 15 has a suction type
Millipore filter 151, a discharge opeing module 152 and stream cleaning module 153 is swept, wherein there is micro- life in the concentration relay unit 13
The stoste of thing can be entered in the suction type millipore filter 151 by the pipeline 15, as shown in Figure 3 B, the negative pressure type micro-hole mistake
Filter 151 is then to include a liquid container, and the negative pressure type micro-hole filter material 1511 for having more than one group is put in the liquid container,
And after the stoste with microorganism enters the suction type millipore filter 151, because the effect of discharge opeing module 152 can pass through the negative pressure
Type micro-hole filter material 1511, but the part with microorganism then can not be by the negative pressure type micro-hole filter material 1511, and this can be stayed in
In suction type millipore filter 151, without microorganism liquid 15112 then can by the negative pressure type micro-hole filter material 1511,
And discharged by the discharge opeing module 152 by the pipeline 17 into the collector unit 20, and the collector unit 20 is a kind of quilt
The dynamic container being collected, therefore when container is expired soon, then the collector unit 20 can be moved, and change a new collector unit
20。
And the negative pressure type micro-hole filter material 1511 can be the hollow fiber form filter membrane of hydrophilic film, wound fil-tration film,
Plate-type filtering film, tubular filter film, disc filter film or other any tool micropores can stop that the filter material of microorganism is formed.
And lasting thickening filtration, the inside of concentration relay unit 13 have the stoste of microorganism can be fewer and fewer, this
As have two kinds of ways, a kind of is that will be unable to return by the concentrate 15111 with microorganism of the negative pressure type micro-hole filter material 1511
It flow in the concentration relay unit 13, to repeat to exclude the liquid 15112 without microorganism, such a way result can be such as
Shown in Fig. 3 C, when stopping filtering, can all have micro- life inside the concentration relay unit 13 and the suction type millipore filter 151
The concentrate 15111 of thing, afterwards, then liquid unit 19 is taken by the concentration relay unit 13 and the suction type millipore filter by this
151 concentrate 15111 with microorganism takes out, and collects storage into the collector unit 20.
It is the concentrate with microorganism not to be back in the concentration relay unit 13 that another kind, which does rule, but foundation
Liquid level minimum point limitation inside the limit that the thickening filtration unit 15 can bear and the concentration relay unit 13, continues defeated
Stoste to thickening filtration unit 15 of microorganism is sent to carry out liquid 15112 of the discharge without microorganism, to the thickening filtration
After the limit that unit 15 can bear, then the action no longer filtered, and when stopping filtering, it can only be declined in the negative pressure
The inside of hole filter 151 retains the concentrate 15111 with microorganism, afterwards, is then taken by this during liquid unit 19 concentrates this
After unit 13 with having the concentrate 15111 of microorganism to take out in the suction type millipore filter 151, and collect storage and extremely should
In collector unit 20.
In addition, in order to maintain the cleaning on the surface of negative pressure type micro-hole filter material 1511 and normal filtering function, to avoid
Microorganism or other suspended solids block the micropore on the surface of negative pressure type micro-hole filter material 1511 during filter, then must be by this
Sweeping stream, cleaning module 153 is washed away with gas jet, liquid, chemical agent brews, heat or ultrasonic vibration comes to suction type
Micropore filter material is cleaned, to avoid the situation of obstruction.
And it is then positive-pressure type that the second of the present invention, which is implemented, as shown in fig. 4 a and fig. 4b, it is have with the first implementation difference
One positive-pressure type millipore filter 154, the positive-pressure type millipore filter 154 are interior with pressure application module 1541 and at least one
Malleation type micro-hole filter material 1542, the difference of suction type millipore filter 151 with first embodiment, it is that mould is applied by the pressure
Surface of the stoste that block 1541 makes to have microorganism with certain flow velocity by the malleation type micro-hole filter material 1542, due to hydrone
By micropore filter material under certain pressure-driven, and suspension, colloid, larger molecular organicses and microorganism etc. be then blocked in
On the film, therefore the concentrate with microorganism can also be stopped by the malleation type micro-hole filter material 1542, without microorganism
Liquid 15422 then can by the malleation type micro-hole filter material 1542, and by the discharge opeing module 152 by the pipeline 17 discharge to
In the collector unit 20, and the collector unit 20 is a kind of container being passively collected, therefore when container is expired soon, then it can remove
The collector unit 20 is moved, and changes a new collector unit 20.
And the malleation type micro-hole filter material 1542 can be the hollow fiber form filter membrane of hydrophilic film, wound fil-tration film,
Plate-type filtering film, tubular filter film, disc filter film, fibrous material filter membrane or other any micropore filter materials are set
It is standby to be formed.
And lasting thickening filtration, the inside of concentration relay unit 13 have the stoste of microorganism also can be fewer and fewer, this
Typically there are two kinds of ways, a kind of is that will be unable to the concentrate 15421 with microorganism by the malleation type micro-hole filter material 1542
It is back in the concentration relay unit 13, to repeat to exclude the liquid 15422 without microorganism, such a way result meeting
As shown in Figure 4 C, when stopping filtering, the inside of positive-pressure type millipore filter 154 can have the concentrate 15421 of microorganism, it
Afterwards, then liquid unit 19 is taken to there is microorganism in the concentration relay unit 13 and the positive-pressure type millipore filter 154 by this
Concentrate 15111 takes out, and collects storage into the collector unit 20.
It is the concentrate with microorganism not to be back in the concentration relay unit 13 that another kind, which does rule, but foundation
Liquid level minimum point limitation inside the limit that the thickening filtration unit 15 can bear and the concentration relay unit 13, continues defeated
Stoste to thickening filtration unit 15 of microorganism is sent to carry out liquid 15422 of the discharge without microorganism, to the thickening filtration
After the limit that unit 15 can bear, then the action no longer filtered, and when stopping filtering, then also can be in the positive-pressure type
The inside of millipore filter 154 retains the concentrate 15421 with microorganism, afterwards, then takes liquid unit 19 to concentrate this by this
Relay unit 13 collects storage extremely with having the concentrate 15111 of microorganism to take out in the positive-pressure type millipore filter 154
In the collector unit 20.
In addition, the present invention in addition to for microorganism more to be concentrated, also has and arranges the liquid without microorganism
The effect of going out, if therefore to obtain the liquid without microorganism, can also be reached by the system.
Microculture thickening filtration system provided by the present invention, when being compared to each other with other prior arts, its advantage
It is as follows：
1st, the stoste with microorganism can be filtered into the highly enriched thing with microorganism and by thickening filtration by the present invention
In unit without microorganism liquid discharge, then can reach make the microorganism of culture separate and concentrate microbial or
Collect the purpose of the liquid without microorganism.
2nd, microorganism of the invention can carry out concentrate simultaneously in the case of condition of culture, and the microorganism energy of the present invention
Enough it is bacterium, algae, fungi or virus.
3rd, by the present invention system, can be saved during microculture thickening filtration more electric power into
This, and more can automatic operation so that overall process is easier to make for manipulating.
The present invention is disclosed as above by the above embodiments, but it is not limited to the present invention, it is any be familiar with this one
Technical field has usually intellectual, passes through the above detailed description of preferred embodiments, it would be desirable to more clearly describe the present invention
Feature and spirit, and not protection scope of the present invention is any limitation as with above-mentioned disclosed preferred embodiment.
On the contrary, the purpose is to wish to cover various changes and have an equality is arranged in the protection domain of the invention to be protected
It is interior.
Claims (29)
- A kind of 1. microculture thickening filtration system, it is characterised in that including：One stoste culture unit, for one to cultivate the container of microorganism, the stoste culture unit has the stoste of microorganism；One concentration relay unit, is connected by least one pipeline with the stoste culture unit, and in the stoste culture unit Stoste with microorganism can be entered in the concentration relay unit by the pipeline；One thickening filtration unit, it is connected by least one pipeline with the concentration relay unit, and in the concentration relay unit Stoste with microorganism can by the pipeline enter the thickening filtration unit in, and the thickening filtration unit to can will Liquid without microorganism is filtered and discharged, to retain the concentrate with higher concentration microorganism.
- 2. microculture thickening filtration system as claimed in claim 1, it is characterised in that the thickening filtration unit at least has There are a suction type millipore filter and a discharge opeing module being connected with the suction type millipore filter, the suction type millipore filter It is connected by least one pipeline with the concentration relay unit, and the stoste with microorganism can in the concentration relay unit Entered by the pipeline in the suction type millipore filter, in the suction type millipore filter there is at least one negative pressure to decline Hole filter material, and should stoste with microorganism by the negative pressure type micro-hole filter material when, should can be by this with concentrate of microorganism Negative pressure type micro-hole filter material blocks, and the discharge opeing module does not then have microorganism by what can be blocked by the negative pressure type micro-hole filter material Liquid discharge.
- 3. microculture thickening filtration system as claimed in claim 2, it is characterised in that the suction type millipore filter energy The stoste with microorganism for enough making to be blocked by the negative pressure type micro-hole filter material is back in the concentration relay unit, and repeats to flow back And be circulated throughout and filter the liquid without microorganism, and can be protected in the concentration relay unit and the suction type millipore filter Stay the concentrate with higher concentration microorganism.
- 4. microculture thickening filtration system as claimed in claim 2, it is characterised in that inside the concentration relay unit Stoste with microorganism continues into the suction type millipore filter and carries out excluding the liquid without microorganism, while in this Retain the concentrate with higher concentration microorganism in suction type millipore filter.
- 5. the microculture thickening filtration system as described in claim 3 or 4, it is characterised in that also taken including at least one Liquid unit and at least one collector unit, wherein, this takes liquid unit can be by the concentration relay unit and the negative pressure type micro-hole mistake Retain the concentrate with higher concentration microorganism in filter to take out and deposit to the collector unit, and the discharge opeing module can also By the liquid discharge storage without microorganism into another collector unit.
- 6. microculture thickening filtration system as claimed in claim 2, it is characterised in that the negative pressure type micro-hole filter material can It is hollow fiber form filter membrane, wound fil-tration film, plate-type filtering film, tubular filter film or the disc filter of hydrophilic film Film is formed.
- 7. microculture thickening filtration system as claimed in claim 2, it is characterised in that also have in the thickening filtration unit Stream cleaning module is swept, can be washed away with gas jet, liquid, chemical agent brews, heat or ultrasonic vibrates next pair Negative pressure type micro-hole filter material is cleaned, to maintain the cleaning of the negative pressure type micro-hole surface of filter medium and normal filtering function, with Microorganism in filter process is avoided to block the micropore of the negative pressure type micro-hole surface of filter medium.
- 8. microculture thickening filtration system as claimed in claim 2, it is characterised in that also relayed including one and the concentration The control unit that unit and the thickening filtration unit are connected, to the management and control concentration relay unit and the thickening filtration unit Filtering operation state.
- 9. microculture thickening filtration system as claimed in claim 8, it is characterised in that also have one in the control unit Micropore blocks monitoring modular, to monitor the pressure and flow of the stoste with microorganism by the negative pressure type micro-hole filter material, To judge micropore degree of congestion.
- 10. microculture thickening filtration system as claimed in claim 8, it is characterised in that also have in the control unit One filtering filtering velocity adjusting module, passes through the filtration pressure during negative pressure type micro-hole filter material to monitor the concentrate with microorganism The concentration of flow velocity or concentrate when power, filtering, to filter the reference frame of filtering velocity as adjustment.
- 11. microculture thickening filtration system as claimed in claim 8, it is characterised in that also have in the control unit One liquid level protection module, to monitor the liquid height of water level inside the concentration relay unit and the suction type millipore filter, And the liquid height of water level is can interpolate that it is negative into this whether the concentration relay unit will continue to convey the stoste of microorganism The processing of the liquid without microorganism is filtered out in pressure type millipore filter.
- 12. microculture thickening filtration system as claimed in claim 8, it is characterised in that also have in the control unit One temperature control module, to monitor and control the fluid temperature inside the concentration relay unit and the suction type millipore filter.
- 13. microculture thickening filtration system as claimed in claim 8, it is characterised in that also have in the control unit One defoaming module, to eliminate the liquid inside the concentration relay unit and the suction type millipore filter because liquid conveys or sweeps Bubble formed in stream process.
- 14. microculture thickening filtration system as claimed in claim 8, it is characterised in that also have in the control unit One automatic cleaning module, can in the state of not operated in the concentration relay unit or the thickening filtration unit Automatically clean inside the concentration relay unit or inside the suction type millipore filter.
- 15. microculture thickening filtration system as claimed in claim 8, it is characterised in that also have in the control unit One leakproof monitoring module, to monitor the state that the discharge opeing module discharges liquid, to avoid this that there is the concentration of microorganism Thing causes the discharge opeing module row to go out with microorganism because the breakage of micropore filter material can not be blocked by the negative pressure type micro-hole filter material completely Liquid.
- 16. microculture thickening filtration system as claimed in claim 1, it is characterised in that the thickening filtration unit includes One positive-pressure type millipore filter and a discharge opeing module being connected with the positive-pressure type millipore filter, the positive-pressure type millipore filter lead to Cross at least one pipeline with the concentration relay unit to be connected, and in the positive-pressure type millipore filter there is a pressure to apply module And at least one malleation type micro-hole filter material, in the concentration relay unit stoste with microorganism can by the pipeline and this just The pressure of pressure type millipore filter applies module connection, so that the stoste with microorganism is under pressure, driving is lower passes through the malleation During type micro-hole filter material, it can should be blocked with the concentrate of microorganism by the malleation type micro-hole filter material, and the discharge opeing module then should The discharge of the liquid without microorganism that malleation type micro-hole filter material does not block.
- 17. microculture thickening filtration system as claimed in claim 16, it is characterised in that the positive-pressure type millipore filter The stoste with microorganism that can make to be blocked by the malleation type micro-hole filter material is back in the concentration relay unit, and is repeated back to Flow and be circulated throughout and filter the liquid with microorganism, then can retain in the concentration relay unit has higher concentration microorganism Concentrate.
- 18. microculture thickening filtration system as claimed in claim 16, it is characterised in that inside the concentration relay unit The stoste with microorganism continue into the positive-pressure type millipore filter carry out exclude without microorganism liquid, then can Retain the concentrate with higher concentration microorganism in the positive-pressure type millipore filter.
- 19. the microculture thickening filtration system as described in claim 17 or 18, it is characterised in that also including at least one Liquid unit and at least one collector unit are taken, wherein this takes liquid unit can be by the concentration relay unit and the malleation type micro-hole mistake Retain the concentrate with higher concentration microorganism in filter to take out and deposit to the collector unit, and the discharge opeing module can also By the liquid discharge storage without microorganism into another collector unit.
- 20. microculture thickening filtration system as claimed in claim 16, it is characterised in that the malleation type micro-hole filter material energy Enough it is hollow fiber form filter membrane, wound fil-tration film, plate-type filtering film, tubular filter film, the disc filter of hydrophilic film Film or fibrous material filter membrane are formed.
- 21. microculture thickening filtration system as claimed in claim 16, it is characterised in that in the thickening filtration unit also With stream cleaning module is swept, can be washed away with gas jet, liquid, chemical agent brews, heat or ultrasonic vibration comes Positive-pressure type micropore filter material is cleaned, to maintain the cleaning of the malleation type micro-hole surface of filter medium and normal filtering function, To avoid microorganism in filter process from blocking the micropore of the malleation type micro-hole surface of filter medium.
- 22. microculture thickening filtration system as claimed in claim 16, it is characterised in that also including in one and the concentration After the control unit that unit and the thickening filtration unit are connected, to the management and control concentration relay unit and the thickening filtration unit Filtering operation state.
- 23. microculture thickening filtration system as claimed in claim 22, it is characterised in that also have in the control unit One micropore blocks monitoring modular, to monitor the pressure and stream of the stoste with microorganism by the malleation type micro-hole filter material Amount, to judge micropore degree of congestion.
- 24. microculture thickening filtration system as claimed in claim 22, it is characterised in that also have in the control unit One filtering filtering velocity adjusting module, passes through the filtration pressure during malleation type micro-hole filter material to monitor the concentrate with microorganism The concentration of flow velocity or concentrate when power, filtering, to filter the reference frame of filtering velocity as adjustment.
- 25. microculture thickening filtration system as claimed in claim 22, it is characterised in that also have in the control unit One liquid level protection module, to monitor the liquid height of water level inside the concentration relay unit, and the liquid height of water level to Can interpolate that the concentration relay unit whether to continue convey microorganism stoste enter the positive-pressure type millipore filter in carry out Filter out the processing of the liquid without microorganism.
- 26. microculture thickening filtration system as claimed in claim 22, it is characterised in that also have in the control unit One temperature control module, to monitor and control the fluid temperature inside the concentration relay unit.
- 27. microculture thickening filtration system as claimed in claim 22, it is characterised in that also have in the control unit One defoaming module, to eliminate the liquid inside the concentration relay unit because liquid conveys or swept the gas formed in stream process Bubble.
- 28. microculture thickening filtration system as claimed in claim 22, it is characterised in that also have in the control unit One automatic cleaning module, can be certainly in the state of not operated in the concentration relay unit or thickening filtration unit It is dynamic to clean inside the concentration relay unit or thickening filtration unit.
- 29. microculture thickening filtration system as claimed in claim 22, it is characterised in that also have in the control unit One leakproof monitoring module, to monitor the state that the discharge opeing module discharges liquid, to avoid this that there is the concentration of microorganism Thing can not be blocked by the malleation type micro-hole filter material completely, and cause the discharge opeing module row to go out the liquid with microorganism.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW105124458 | 2016-08-02 | ||
| TW105124458A TWI648398B (en) | 2016-08-02 | 2016-08-02 | Microbial culture concentration filtration system |
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| Publication Number | Publication Date |
|---|---|
| CN107674825A true CN107674825A (en) | 2018-02-09 |
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|---|---|---|---|
| CN201720944740.XU Expired - Fee Related CN207159211U (en) | 2016-08-02 | 2017-07-31 | Microorganism culture concentration filtration system |
| CN201710641531.2A Pending CN107674825A (en) | 2016-08-02 | 2017-07-31 | Microorganism culture concentration filtration system |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201720944740.XU Expired - Fee Related CN207159211U (en) | 2016-08-02 | 2017-07-31 | Microorganism culture concentration filtration system |
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| CN (2) | CN207159211U (en) |
| TW (1) | TWI648398B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2615635A (en) * | 2021-12-15 | 2023-08-16 | Idex Health & Science Llc | Automated sample preparation for spent media analysis |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113634125A (en) * | 2021-10-18 | 2021-11-12 | 深圳市路阳农业科技有限公司 | Alkaline microorganism culture solution concentration and filtration device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102325598A (en) * | 2008-12-31 | 2012-01-18 | 3M创新有限公司 | Methods, kits and systems for processing samples |
| CN203741336U (en) * | 2014-03-27 | 2014-07-30 | 中国人民解放军军事医学科学院军事兽医研究所 | Biological safety type zymophyte liquid hollow fiber ultrafiltration system |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8026096B1 (en) * | 1998-10-08 | 2011-09-27 | Protein Sciences Corporation | In vivo active erythropoietin produced in insect cells |
| JP5659466B2 (en) * | 2009-08-07 | 2015-01-28 | 東レ株式会社 | Method and apparatus for producing chemicals by continuous culture |
| TWM538945U (en) * | 2016-08-02 | 2017-04-01 | Iwater Tech Inc | Microbial culture and enrichment and filtration system |
-
2016
- 2016-08-02 TW TW105124458A patent/TWI648398B/en active
-
2017
- 2017-07-31 CN CN201720944740.XU patent/CN207159211U/en not_active Expired - Fee Related
- 2017-07-31 CN CN201710641531.2A patent/CN107674825A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102325598A (en) * | 2008-12-31 | 2012-01-18 | 3M创新有限公司 | Methods, kits and systems for processing samples |
| CN203741336U (en) * | 2014-03-27 | 2014-07-30 | 中国人民解放军军事医学科学院军事兽医研究所 | Biological safety type zymophyte liquid hollow fiber ultrafiltration system |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2615635A (en) * | 2021-12-15 | 2023-08-16 | Idex Health & Science Llc | Automated sample preparation for spent media analysis |
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| Publication number | Publication date |
|---|---|
| CN207159211U (en) | 2018-03-30 |
| TWI648398B (en) | 2019-01-21 |
| TW201809257A (en) | 2018-03-16 |
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