TWM538945U - Microbial culture and enrichment and filtration system - Google Patents
Microbial culture and enrichment and filtration system Download PDFInfo
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本創作是有關一種微生物培養濃縮過濾系統，特別是一種可以濃縮微生物培養液或產生無微生物過濾液的微生物培養濃縮過濾系統。The present invention relates to a microbial culture concentrated filtration system, in particular to a microbial culture concentrated filtration system capable of concentrating a microbial culture solution or producing a microbial-free filtration solution.
微生物發酵包含有液態發酵及固態發酵兩種，其中液態發酵的發酵品質較為穩定，且發酵速度快，可在短時間內大量生產，一般可分為「一次培養法」、「分批補料培養法」及「連續培養法」，其中一次培養法是一次將所有原料和微生物加入培養桶中培養，直到培養完成後，再將內容物全數取出的傳統培養方法；Microbial fermentation includes liquid fermentation and solid fermentation. The fermentation quality of liquid fermentation is relatively stable, and the fermentation speed is fast. It can be mass produced in a short time. Generally, it can be divided into "primary culture method" and "batch feed culture. The method and the "continuous culture method", wherein the primary culture method is a conventional culture method in which all the raw materials and microorganisms are added to the culture tank until the culture is completed, and then the contents are completely taken out;
而「分批補料培養法」是指在微生物不同生長時期，分批地間歇或連續補充新鮮培養材料方法。所補的原料可以是全料，也可以是氮源、碳源等，目的是延長代謝產物的合成時間以達到更大的量產或是控制特定產品的品質等等；The "batch fed culture method" refers to a method of intermittently or continuously replenishing fresh culture materials in batches at different growth stages of microorganisms. The raw materials to be supplemented may be all materials, or may be nitrogen sources, carbon sources, etc., in order to prolong the synthesis time of metabolites to achieve greater mass production or control the quality of specific products, and the like;
而「連續培養法」是指在發酵桶加入新培養液的同時，也採收原有培養液的發酵方法。其優點是設備利用率高、產品質量穩定，便於自動控制等，缺點是容易污染雜菌。通常只適合對環境有極大適應能力的強勢微生物，如醋酸菌。The "continuous culture method" refers to a fermentation method in which a new culture solution is added to a fermenter while the original culture solution is also recovered. The advantages are high equipment utilization, stable product quality, easy automatic control, etc. The disadvantage is that it is easy to contaminate the bacteria. It is usually only suitable for strong microorganisms that have great adaptability to the environment, such as acetic acid bacteria.
而微生物除了上述提到的細菌之外，有些藻類亦屬於微生物，由於藻類是自營性生物，二氧化碳為輔助生長的碳源，所以越有效率的培養系統，其藻類利用二氧化碳的效率也越高。藻類培養系統大致可分為開放系統與密閉系統兩種，既知的藻類開放式培養系統主要有圓形培養池（Circular pond）及跑道式培養池（Race-way pond）兩種。In addition to the bacteria mentioned above, some algae are also microbes. Since algae are self-supporting organisms and carbon dioxide is a carbon source for growth, the more efficient the culture system, the more efficient the algae can use carbon dioxide. . The algae cultivation system can be roughly divided into an open system and a closed system. The known algae open culture system mainly has two types: a circular pond and a race-way pond.
由於目前工業上多使用全自動控制發酵槽來進行微生物的液態發酵，其裝置包含有一體成型的發酵槽的控制監測設備以及發酵槽槽體，目的是為了避免外界的污染以及使用上的方便；但有一最大問題是如何將培養液與微生物分離，並使微生物逐漸純化，另外就是過去一般多使用離心的方法進行處理，但這種處理方法能夠處理的溶液量有限，且效率也不高。Since the industrial automatic fermentation tank is used to carry out liquid fermentation of microorganisms, the device comprises a control monitoring device for the integrated fermentation tank and a fermentation tank tank, in order to avoid external pollution and convenience in use; However, one of the biggest problems is how to separate the culture solution from the microorganisms and gradually purify the microorganisms. In addition, in the past, the method of centrifugation was generally used for treatment, but the amount of the solution which can be treated by this treatment method is limited and the efficiency is not high.
因此，為了克服上述問題，若能夠藉由一濃縮過濾單元將具有微生物之原液進行過濾並排出不具有微生物之液體，則能夠同時達到使培養之微生物分離出來並被濃縮，同時產生無微生物之培養液的目的，如此應為一最佳解決方案。Therefore, in order to overcome the above problem, if a liquid solution containing microorganisms can be filtered by a concentrated filtration unit and a liquid having no microorganisms can be discharged, the microorganisms which are cultured can be simultaneously separated and concentrated, and a microorganism-free culture can be produced at the same time. The purpose of the liquid should be an optimal solution.
一種微生物培養濃縮過濾系統，係包含：一原液培養單元，係為一用以培養微生物之容器，該原液培養單元係具有微生物之原液；一濃縮中繼單元，係透過至少一個管路與該原液培養單元相連接，而該原液培養單元內具有微生物之原液能夠通過該管路進入該濃縮中繼單元中；一濃縮過濾單元，係透過至少一個管路與該濃縮中繼單元相連接，而該濃縮中繼單元內具有微生物之原液能夠通過該管路進入該濃縮過濾單元中，而該濃縮過濾單元用以能夠將不具有微生物之液體過濾並排出，以保留具有較高濃度微生物之濃縮物。A microbial culture concentrated filtration system comprises: a raw liquid culture unit, which is a container for cultivating microorganisms, wherein the raw liquid culture unit has a raw material of microorganisms; and a concentrated relay unit transmits the liquid through at least one pipeline The culture unit is connected, and the raw liquid having the microorganism in the raw liquid culture unit can enter the concentrated relay unit through the pipeline; and the concentrated filtration unit is connected to the concentrated relay unit through at least one pipeline, and the The raw liquid having microorganisms in the concentration relay unit can enter the concentrated filtration unit through the pipeline, and the concentrated filtration unit is configured to be capable of filtering and discharging the liquid without microorganisms to retain the concentrate having a higher concentration of microorganisms.
更具體的說，所述濃縮過濾單元係至少具有一負壓式微孔過濾器及一與該負壓式微孔過濾器連接之排液模組，該負壓式微孔過濾器係透過至少一個管路與該濃縮中繼單元相連接，而該濃縮中繼單元內具有微生物之原液能夠通過該管路進入該負壓式微孔過濾器中，該負壓式微孔過濾器中係具有至少一個的負壓式微孔濾材，而該具有微生物之原液通過該負壓式微孔濾材時，該具有微生物之濃縮物會被該負壓式微孔濾材攔阻，而該排液模組則將可通過該負壓式微孔濾材沒有攔阻之不具有微生物之液體排出。More specifically, the concentrated filter unit has at least one negative pressure microporous filter and a liquid discharge module connected to the negative pressure microporous filter, and the negative pressure microporous filter transmits at least a pipeline connected to the concentrating relay unit, and a raw liquid having microorganisms in the concentrating relay unit can enter the negative pressure microporous filter through the pipeline, wherein the negative pressure microporous filter has At least one negative pressure microporous filter material, and when the microbial stock solution passes through the negative pressure microporous filter material, the microbial concentrate is blocked by the negative pressure microporous filter material, and the liquid discharge module is The liquid without microorganisms which can be blocked by the negative pressure microporous filter material is discharged.
更具體的說，所述負壓式微孔過濾器能夠使被該負壓式微孔濾材攔阻之具有微生物之原液回流至該濃縮中繼單元中，而重複回流並循環過濾掉不具有微生物之液體，並能夠於該濃縮中繼單元及該負壓式微孔過濾器中保留具有較高濃度微生物之濃縮物。More specifically, the negative pressure microporous filter can return the microbial stock solution blocked by the negative pressure microporous filter material to the concentrated relay unit, and repeat the reflux and circulate and filter out the microorganisms. a liquid and capable of retaining a concentrate having a higher concentration of microorganisms in the concentrated relay unit and the negative pressure microporous filter.
更具體的說，所述濃縮中繼單元內部之具有微生物之原液持續進入該負壓式微孔過濾器進行排除不具有微生物之液體，同時於該負壓式微孔過濾器中保留具有較高濃度微生物之濃縮物。More specifically, the microbial stock solution inside the concentrated relay unit continuously enters the negative pressure microporous filter to remove the liquid without microorganisms, and remains high in the negative pressure microporous filter. Concentrate of concentrated microorganisms.
更具體的說，所述微生物培養濃縮過濾系統，更包含有至少一個取液單元及至少一個收集單元，其中該取液單元能夠將該該濃縮中繼單元及該負壓式微孔過濾器中保留具有較高濃度微生物之濃縮物取出並存放至該收集單元，而該排液模組更能夠將不具有微生物之液體排出存放至另一個收集單元中。More specifically, the microbial culture concentrated filtration system further includes at least one liquid take-out unit and at least one collection unit, wherein the liquid take-up unit can be used in the concentrated relay unit and the negative pressure microporous filter The concentrate having the higher concentration of microorganisms is taken out and stored in the collection unit, and the liquid discharge module is more capable of discharging the liquid without microorganisms into another collection unit.
更具體的說，所述負壓式微孔濾材係能夠為親水性薄膜的中空纖維式過濾膜、捲式過濾膜、板式過濾膜、管式過濾膜、碟片式過濾膜或是其他任何可阻擋微生物之微孔濾材所組成。More specifically, the negative pressure microporous filter material can be a hollow fiber filter membrane, a roll filter membrane, a plate filter membrane, a tubular filter membrane, a disc filter membrane or any other which can be a hydrophilic membrane. It consists of a microporous filter material that blocks microorganisms.
更具體的說，所述濃縮過濾單元中更具有一掃流清洗模組，能夠以氣體噴氣、液體沖刷、化學藥劑沖泡、加溫或是超音波振盪來對負壓式微孔濾材進行清洗，用以維持該負壓式微孔濾材表面的清潔與正常的過濾功能，以避免過濾過程中微生物阻塞住該負壓式微孔濾材表面的微孔。More specifically, the concentrating filter unit further has a sweep cleaning module capable of cleaning the negative pressure microporous filter material by gas jet, liquid scour, chemical brewing, heating or ultrasonic oscillation. The utility model is used for maintaining the clean and normal filtering function of the surface of the negative pressure microporous filter material, so as to prevent microorganisms from blocking the micropores on the surface of the negative pressure microporous filter material during the filtration process.
更具體的說，所述微生物培養濃縮過濾系統，更包含有一與該濃縮中繼單元及該濃縮過濾單元相連接之控制單元，用以管控該濃縮中繼單元及該濃縮過濾單元之過濾運作狀態。More specifically, the microbial culture concentrated filtration system further includes a control unit connected to the concentrated relay unit and the concentrated filter unit for controlling the filtration operation state of the concentrated relay unit and the concentrated filter unit. .
更具體的說，所述控制單元中更具有一微孔阻塞監測模組，用以偵測通過該負壓式微孔濾材之具有微生物之原液的壓力與流量，以判斷微孔阻塞程度。More specifically, the control unit further includes a micro-hole blocking monitoring module for detecting the pressure and flow rate of the microbial stock solution passing through the negative-pressure microporous filter material to determine the degree of micro-hole blocking.
更具體的說，所述控制單元中更具有一過濾濾速調整模組，用以偵測具有微生物之濃縮物通過該負壓式微孔濾材時的過濾壓力、過濾時的流速或是濃縮物的濃度，以做為調整過濾濾速的參考依據。More specifically, the control unit further has a filter filter speed adjustment module for detecting the filtration pressure of the microbial concentrate through the negative pressure microporous filter material, the flow rate during the filtration, or the concentrate. The concentration is used as a reference for adjusting the filtration rate.
更具體的說，所述控制單元中更具有一液位保護模組，用以偵測該濃縮中繼單元及該負壓式微孔過濾器內部之液體水位高度，而該液體水位高度用以能夠判斷該濃縮中繼單元是否要繼續輸送含微生物之原液進入該負壓式微孔過濾器中進行過濾掉不具有微生物之液體的處理。More specifically, the control unit further has a liquid level protection module for detecting the liquid level of the liquid inside the concentrated relay unit and the negative pressure microporous filter, and the liquid water level is used for It is possible to determine whether the concentrated relay unit is to continue to transport the microbial-containing stock solution into the negative pressure microporous filter to filter out the liquid having no microorganisms.
更具體的說，所述控制單元中更具有一溫控模組，用以偵測並控制該濃縮中繼單元及該負壓式微孔過濾器內部之液體溫度。More specifically, the control unit further includes a temperature control module for detecting and controlling the temperature of the liquid inside the concentrated relay unit and the negative pressure microporous filter.
更具體的說，所述控制單元中更具有一消泡模組，用以消除該濃縮中繼單元及該負壓式微孔過濾器內部之液體因液體輸送或掃流過程中所形成的氣泡。More specifically, the control unit further includes a defoaming module for eliminating bubbles formed by the liquid in the concentrated relay unit and the negative pressure microporous filter due to liquid transportation or sweeping. .
更具體的說，所述控制單元中更具有一自動清洗模組，用以於該濃縮中繼單元及該濃縮過濾單元沒有進行運作的狀態下，能夠自動清洗該濃縮中繼單元內部及該負壓式微孔過濾器內部。More specifically, the control unit further includes an automatic cleaning module for automatically cleaning the inside of the concentrated relay unit and the negative state when the concentrated relay unit and the concentrated filtering unit are not operating. Pressurized microporous filter inside.
更具體的說，所述控制單元中更具有一防漏監控模組，用以監控該排液模組所排出液體的狀態，用以避免該具有微生物之濃縮物因微孔濾材破損無法完全被該負壓式微孔濾材攔阻，而導致該排液模組排出具有微生物之液體。More specifically, the control unit further has a leakproof monitoring module for monitoring the state of the liquid discharged by the liquid discharging module, so as to prevent the microbial concentrate from being completely damaged by the microporous filter material. The negative pressure microporous filter material blocks, and the liquid discharge module discharges the liquid having microorganisms.
更具體的說，所述濃縮過濾單元係包含有一正壓式微孔過濾器及一與該正壓式微孔過濾器連接之排液模組，該正壓式微孔過濾器係透過至少一個管路與該濃縮中繼單元相連接，而該正壓式微孔過濾器內係具有一壓力施加模組及至少一個正壓式微孔濾材，該濃縮中繼單元內具有微生物之原液能夠通過該管路與該正壓式微孔過濾器之壓力施加模組連接，以使具有微生物之原液受到壓力驅動下通過該正壓式微孔濾材時，該具有微生物之濃縮物會被該正壓式微孔濾材攔阻，而該排液模組則將該正壓式微孔濾材沒有攔阻之不具有微生物之液體排出。More specifically, the concentrated filter unit comprises a positive pressure microporous filter and a liquid discharge module connected to the positive pressure microporous filter, and the positive pressure microporous filter transmits at least one The pipeline is connected to the concentrating relay unit, and the positive pressure microporous filter has a pressure application module and at least one positive pressure microporous filter material, and the microbial liquid solution in the concentrating relay unit can pass through The pipeline is connected to the pressure application module of the positive pressure microporous filter, so that the microbial concentrate is subjected to the positive pressure when the microbial stock solution is pressure-driven through the positive pressure microporous filter material. The microporous filter material blocks, and the liquid discharge module discharges the liquid without microorganisms which is not blocked by the positive pressure microporous filter material.
更具體的說，所述正壓式微孔過濾器能夠使被該正壓式微孔濾材攔阻之具有微生物之原液回流至該濃縮中繼單元中，而重複回流並循環過濾掉不具有微生物之液體，則能夠於該濃縮中繼單元中保留具有較高濃度微生物之濃縮物。More specifically, the positive pressure microporous filter can return the microbial stock solution blocked by the positive pressure microporous filter material to the concentrated relay unit, and repeat the reflux and circulate and filter out the microorganisms. The liquid is capable of retaining a concentrate having a higher concentration of microorganisms in the concentrated relay unit.
更具體的說，所述濃縮中繼單元內部之具有微生物之原液持續進入該正壓式微孔過濾器進行排除不具有微生物之液體，則能夠於該正壓式微孔過濾器中保留具有較高濃度微生物之濃縮物。More specifically, the microbial stock solution inside the concentrating relay unit continuously enters the positive pressure microporous filter to remove the liquid without microorganisms, and can be retained in the positive pressure microporous filter. Concentrate of high concentration microorganisms.
更具體的說，所述微生物培養濃縮過濾系統，更包含有至少一個取液單元及至少一個收集單元，其中該取液單元能夠將該該濃縮中繼單元及該負壓式微孔過濾器中保留具有較高濃度微生物之濃縮物取出並存放至該收集單元，而該排液模組更能夠將不具有微生物之液體排出存放至另一個收集單元中。More specifically, the microbial culture concentrated filtration system further includes at least one liquid take-out unit and at least one collection unit, wherein the liquid take-up unit can be used in the concentrated relay unit and the negative pressure microporous filter The concentrate having the higher concentration of microorganisms is taken out and stored in the collection unit, and the liquid discharge module is more capable of discharging the liquid without microorganisms into another collection unit.
更具體的說，所述正壓式微孔濾材係能夠為親水性薄膜的中空纖維式過濾膜、捲式過濾膜、板式過濾膜、管式過濾膜、碟片式過濾膜或是纖維性材料過濾膜所組成。More specifically, the positive pressure microporous filter material can be a hollow fiber filter membrane, a roll filter membrane, a plate filter membrane, a tubular filter membrane, a disc filter membrane or a fibrous material which is a hydrophilic membrane. The composition of the filter membrane.
更具體的說，所述濃縮過濾單元中更具有一掃流清洗模組，能夠以氣體噴氣、液體沖刷、化學藥劑沖泡、加溫或是超音波振盪來對正壓式微孔濾材進行清洗，用以維持該正壓式微孔濾材表面的清潔與正常的過濾功能，以避免過濾過程中微生物阻塞住該正壓式微孔濾材表面的微孔。More specifically, the concentrated filter unit further has a sweep cleaning module capable of cleaning the positive pressure microporous filter material by gas jet, liquid flushing, chemical brewing, heating or ultrasonic oscillation. The utility model is used for maintaining the clean and normal filtering function of the surface of the positive pressure microporous filter material, so as to prevent microorganisms from blocking the micropores on the surface of the positive pressure microporous filter material during the filtration process.
更具體的說，所述微生物培養濃縮過濾系統，更包含有一與該濃縮中繼單元及該濃縮過濾單元相連接之控制單元，用以管控該濃縮中繼單元及該濃縮過濾單元之過濾運作狀態。More specifically, the microbial culture concentrated filtration system further includes a control unit connected to the concentrated relay unit and the concentrated filter unit for controlling the filtration operation state of the concentrated relay unit and the concentrated filter unit. .
更具體的說，所述控制單元中更具有一微孔阻塞監測模組，用以偵測通過該正壓式微孔濾材之具有微生物之原液的壓力與流量，以判斷微孔阻塞程度。More specifically, the control unit further includes a micro-hole blocking monitoring module for detecting the pressure and flow rate of the microbial stock solution passing through the positive-pressure microporous filter material to determine the degree of micro-hole blocking.
更具體的說，所述控制單元中更具有一過濾濾速調整模組，用以偵測具有微生物之濃縮物通過該正壓式微孔濾材時的過濾壓力、過濾時的流速或是濃縮物的濃度，以做為調整過濾濾速的參考依據。More specifically, the control unit further has a filter speed adjustment module for detecting the filtration pressure of the microbial concentrate through the positive pressure microporous filter material, the flow rate during the filtration, or the concentrate. The concentration is used as a reference for adjusting the filtration rate.
更具體的說，所述控制單元中更具有一液位保護模組，用以偵測該濃縮中繼單元內部之液體水位高度，而該液體水位高度用以能夠判斷該濃縮中繼單元是否要繼續輸送微生物之原液進入該正壓式微孔過濾器中進行過濾掉不具有微生物之液體的處理。More specifically, the control unit further includes a liquid level protection module for detecting the liquid water level inside the concentrated relay unit, and the liquid water level is used to determine whether the concentrated relay unit is to be The process of transporting the microbial stock solution into the positive pressure microporous filter to filter out the liquid without microorganisms is continued.
更具體的說，所述控制單元中更具有一溫控模組，用以偵測並控制該濃縮中繼單元內部之液體溫度。More specifically, the control unit further includes a temperature control module for detecting and controlling the temperature of the liquid inside the concentrating relay unit.
更具體的說，所述控制單元中更具有一消泡模組，用以消除該濃縮中繼單元內部之液體因液體輸送或掃流過程中所形成的氣泡。More specifically, the control unit further includes a defoaming module for eliminating air bubbles formed in the liquid inside the concentrated relay unit due to liquid transportation or sweeping.
更具體的說，所述控制單元中更具有一自動清洗模組，用以於該濃縮中繼單元或濃縮過濾單元沒有進行運作的狀態下，能夠自動清洗該濃縮中繼單元或濃縮過濾單元內部。More specifically, the control unit further includes an automatic cleaning module for automatically cleaning the concentrated relay unit or the concentrated filter unit in a state where the concentrated relay unit or the concentrated filter unit is not operating. .
更具體的說，所述控制單元中更具有一防漏監控模組，用以監控該排液模組所排出液體的狀態，用以避免該具有微生物之濃縮物無法完全被該正壓式微孔濾材攔阻，而導致該排液模組排出具有微生物之液體。More specifically, the control unit further has a leakproof monitoring module for monitoring the state of the liquid discharged by the liquid discharging module, so as to prevent the microbial concentrate from being completely replaced by the positive pressure type. The pore filter material blocks, causing the liquid discharge module to discharge the liquid having microorganisms.
有關於本創作其他技術內容、特點與功效，在以下配合參考圖式之較佳實施例的詳細說明中，將可清楚的呈現。Other technical contents, features, and effects of the present invention will be apparent from the following detailed description of the preferred embodiments.
請參閱第1圖及第2圖，為本創作微生物培養濃縮過濾系統整體架構示意圖及控制單元內部架構示意圖，如圖中所示，該微生物培養濃縮過濾系統1係至少包含一原液培養單元11、一濃縮中繼單元13、一濃縮過濾單元15、一控制單元18、一取液模組19及一收集模組20，而該原液培養單元11、濃縮中繼單元13、濃縮過濾單元15、控制單元18之間係透過該管路12,13,14,16,17進行液體輸送；Please refer to FIG. 1 and FIG. 2 , which are schematic diagrams of the overall structure of the microbial culture concentrated filtration system and the internal structure of the control unit. As shown in the figure, the microbial culture concentrated filtration system 1 includes at least one stock culture unit 11 . a concentration relay unit 13, a concentration filter unit 15, a control unit 18, a liquid take-out module 19 and a collection module 20, and the stock solution culture unit 11, the concentration relay unit 13, the concentration filter unit 15, and the control The cells 18 are transported through the tubes 12, 13, 14, 16, 17 for liquid transport;
其中該原液培養單元11係為一用以培養微生物之容器，而該原液培養單元係具有微生物之原液；其中該濃縮中繼單元13係透過該管路12與該原液培養單元11相連接，以使該原液培養單元11內具有微生物之原液則能夠通過該管路12進入該濃縮中繼單元13中；The stock solution culture unit 11 is a container for cultivating microorganisms, and the stock solution culture unit has a stock solution of microorganisms; wherein the concentrated relay unit 13 is connected to the stock solution culture unit 11 through the line 12 to The raw liquid having microorganisms in the raw liquid culture unit 11 can enter the concentration relay unit 13 through the pipeline 12;
其中該濃縮過濾單元15則是通過該管路14與該濃縮中繼單元13相連接，以使該濃縮中繼單元13內具有微生物之原液能夠通過該管路14進入該濃縮過濾單元15中，由於該濃縮過濾單元15內部係具有至少一組濾材，因此該濃縮過濾單元15則能夠將不具有微生物之液體過濾並由該管路17排出，並保留具有較高濃度微生物之濃縮物，而具有較高濃度微生物之濃縮物能夠在通過該管路16排回至該濃縮中繼單元13，而重複過濾與回流的程序將能夠使該濃縮中繼單元13內的原液所含有的微生物濃度越來越高。The concentrated filter unit 15 is connected to the concentrating relay unit 13 through the pipeline 14 so that the raw liquid having microorganisms in the concentrating relay unit 13 can enter the concentrating filter unit 15 through the pipeline 14 . Since the concentrated filter unit 15 has at least one set of filter materials inside, the concentrated filter unit 15 is capable of filtering and discharging the liquid without microorganisms, and retaining the concentrate having a higher concentration of microorganisms, and having The concentrate of the higher concentration microorganisms can be discharged back to the concentration relay unit 13 through the line 16, and the repeated filtration and reflux procedures will enable the concentration of the microorganisms contained in the stock solution in the concentration relay unit 13 to become The higher.
而該控制單元18係用以管控該濃縮中繼單元13及該濃縮過濾單元15之過濾運作狀態，該控制單元18中係具有一微孔阻塞監測模組181、一過濾濾速調整模組182、一液位保護模組183、一溫控模組184、一消泡模組185、一自動清洗模組186及一防漏監控模組187；The control unit 18 is configured to control the filtering operation state of the concentrating relay unit 13 and the concentrating filter unit 15. The control unit 18 has a micro-hole blocking monitoring module 181 and a filtering filter speed adjusting module 182. a liquid level protection module 183, a temperature control module 184, a defoaming module 185, an automatic cleaning module 186 and a leak detection module 187;
其中該微孔阻塞監測模組181用以偵測具有微生物之原液通過該濃縮過濾單元15中之濾材時的壓力與流量，以判斷微孔阻塞程度，而該過濾濾速調整模組182則是用以偵測具有微生物之濃縮物通過該濃縮過濾單元15中之濾材時的過濾壓力、過濾時的流速或是濃縮物的濃度，以做為調整過濾濾速的參考依據；The micro-hole blocking monitoring module 181 is configured to detect the pressure and flow rate of the raw material having the microorganisms passing through the filter medium in the concentrated filtering unit 15 to determine the degree of micro-hole blocking, and the filtering rate adjusting module 182 is The method is used for detecting the filtration pressure, the flow rate during filtration or the concentration of the concentrate when the concentrate having the microorganism passes through the filter medium in the concentrated filter unit 15 as a reference for adjusting the filtration speed;
而該液位保護模組183則是用以偵測液體水位高度，而該液體水位高度能夠判斷濃縮中繼單元13是否要繼續輸送具微生物之原液進入該濃縮過濾單元15中進行過濾掉不具有微生物之液體的處理；該液位保護模組183還可以判斷濃縮過濾單元15中的液位水位，用以保護微孔濾材不會因為液體水位過低而造成損壞或是決定排出濃縮物的停止動作；The liquid level protection module 183 is configured to detect the liquid water level height, and the liquid water level height can determine whether the concentrated relay unit 13 continues to transport the microbial stock solution into the concentrated filter unit 15 for filtering. The liquid level protection module 183 can also determine the liquid level in the concentrated filter unit 15 to protect the microporous filter material from damage due to the liquid water level being too low or to determine the discharge of the concentrate. action;
而該溫控模組184主要用以偵測及控制該濃縮中繼單元13及濃縮過濾單元15中之液體溫度；另外該消泡模組185則是用以消除該濃縮中繼單元13內部之液體因液體輸送過程中所形成的氣泡及該濃縮過濾單元15中因液體輸送或清洗掃流過程中所形成的氣泡；The temperature control module 184 is mainly used to detect and control the temperature of the liquid in the concentrating relay unit 13 and the concentrating filter unit 15; and the defoaming module 185 is used to eliminate the internal concentrating unit 13 The liquid formed by the liquid during the liquid transport process and the bubbles formed in the concentrated filter unit 15 during the liquid transport or cleaning sweep;
而該自動清洗模組185用以於該濃縮中繼單元13及該濃縮過濾單元15沒有進行運作的狀態下，能夠自動清洗該濃縮中繼單元13內部及該濃縮過濾單元15的濾材、容器內部及管路。另外為了避免該具有微生物之濃縮物無法完全被該濃縮過濾單元15內部的濾材攔阻，而導致該排出具有微生物之液體，因此必須透過該防漏監控模組187進行監控該所排出液體的狀態，若是濁度過高，則判斷該濃縮過濾單元15內部的濾材可能有破損漏洞或是其他導致過濾失敗的問題發生。The automatic cleaning module 185 is configured to automatically clean the inside of the concentrating relay unit 13 and the filter medium and the inside of the concentrating filter unit 15 while the concentrating relay unit 13 and the concentrating filter unit 15 are not operating. And piping. In addition, in order to prevent the microbial concentrate from being completely blocked by the filter material inside the concentrated filter unit 15, the liquid having the microorganisms is discharged, so the state of the discharged liquid must be monitored through the leak prevention monitoring module 187. If the turbidity is too high, it is judged that the filter material inside the concentrated filter unit 15 may have a damage hole or other problems that cause filtration failure.
而該濃縮過濾單元15能夠有兩種形式，第一種則是負壓式，而第二種則是正壓式，本創作之第一實施則是負壓式，如第3A圖及第3B圖所示，該濃縮過濾單元15內部係具有一負壓式微孔過濾器151、一排液模組152及一掃流清洗模組153，其中該濃縮中繼單元13內具有微生物之原液能夠通過該管路15進入該負壓式微孔過濾器151中，如第3B圖所示，該負壓式微孔過濾器151則是包含一個液體容器，且該液體容器中係置放有一組以上的負壓式微孔濾材1511，而具有微生物之原液進入該負壓式微孔過濾器151後，因排液模組152的作用會通過該負壓式微孔濾材1511，但具有微生物的部分則無法通過該負壓式微孔濾材1511，而會停留於該負壓式微孔過濾器151內，而不具有微生物的液體15112則會通過該負壓式微孔濾材1511，並由該排液模組152透過該管路17向外排出至該收集單元20中，而該收集單元20是一種被動進行收集的容器，故於容器快滿時，則能夠搬移該收集單元20，並更換一個新的收集單元20；The concentrated filter unit 15 can have two forms, the first one is a negative pressure type, and the second type is a positive pressure type, and the first implementation of the present invention is a negative pressure type, such as 3A and 3B. As shown in the figure, the concentrating filter unit 15 has a negative pressure microporous filter 151, a drain module 152 and a sweep cleaning module 153, wherein the concentrating relay unit 13 has a microbial liquid solution capable of passing through The pipe 15 enters the negative pressure microporous filter 151. As shown in FIG. 3B, the negative pressure microporous filter 151 includes a liquid container, and the liquid container is provided with one or more sets. The negative pressure microporous filter material 1511, and after the microbial stock solution enters the negative pressure microporous filter 151, the negative pressure microporous filter material 1511 passes through the liquid discharge module 152, but has a microbial portion. Then, the negative pressure microporous filter material 1511 cannot pass through the negative pressure microporous filter 151, and the liquid 15112 without microorganisms passes through the negative pressure microporous filter material 1511, and the row is The liquid module 152 is discharged to the collection unit 20 through the line 17, and 20 is a passive collection unit for collecting container, so the container is almost full at the time, it is possible to move the collecting unit 20, and replaced with a new collection unit 20;
而該負壓式微孔濾材1511係能夠為親水性薄膜的中空纖維式過濾膜、捲式過濾膜、板式過濾膜、管式過濾膜、碟片式過濾膜或其他任何具微孔可阻擋微生物的濾材所組成；The negative pressure microporous filter material 1511 can be a hollow fiber filter membrane, a roll filter membrane, a plate filter membrane, a tubular filter membrane, a disc filter membrane or any other microporous barrier microorganism capable of being a hydrophilic membrane. Composition of the filter material;
而持續的濃縮過濾，該濃縮中繼單元13內部具有微生物之原液會越來越少，這一般有兩種做法，一種是將無法通過該負壓式微孔濾材1511之具有微生物的濃縮物15111回流至該濃縮中繼單元13中，以重複進行排除不具有微生物的液體15112，此種做法結果會如第3C圖所示，於停止過濾時，該濃縮中繼單元13與該負壓式微孔過濾器151內部皆會具有微生物的濃縮物15111，之後，則由該取液單元19將該濃縮中繼單元13與該負壓式微孔過濾器151的具有微生物的濃縮物15111取出，並收集存放至該收集單元20中；With continuous concentrated filtration, the concentration of the microorganisms in the concentrated relay unit 13 will be less and less, which generally has two kinds of practices, one is that the microbial concentrate 15111 which cannot pass the negative pressure microporous filter material 1511. Returning to the concentrating relay unit 13 to repeatedly remove the liquid 15112 having no microorganisms, the result of which is as shown in FIG. 3C, when the filtration is stopped, the concentrating relay unit 13 and the negative pressure type micro The pore filter 151 has a microbial concentrate 15111 inside, and then the concentration relay unit 13 and the microbial concentrate 15111 of the negative pressure microporous filter 151 are taken out by the liquid take-out unit 19, and Collecting and storing in the collecting unit 20;
另一種做法則是不將具有微生物的濃縮物回流至該濃縮中繼單元13中，而是依據該濃縮過濾單元15所能夠負擔的極限及該濃縮中繼單元13內部的液位最低點限制，持續輸送微生物之原液至該濃縮過濾單元15進行排出不具有微生物的液體15112，到該濃縮過濾單元15所能夠負擔的極限後，則不再進行過濾的動作，而於停止過濾時，僅會於該負壓式微孔過濾器151內部保留該具有微生物的濃縮物15111，之後，則由該取液單元19將該濃縮中繼單元13與該負壓式微孔過濾器151中所具有微生物的濃縮物15111取出，並收集存放至該收集單元20中。Alternatively, the concentrate having the microorganisms is not returned to the concentration relay unit 13, but according to the limit that the concentrated filter unit 15 can bear and the minimum point limit of the liquid inside the concentration relay unit 13, The raw liquid of the microorganism is continuously transported to the concentrated filtration unit 15 to discharge the liquid 15112 having no microorganisms, and after the limit that the concentrated filtration unit 15 can bear, the filtering operation is not performed, and when the filtration is stopped, only the filtration is stopped. The microporous concentrate 15111 is retained inside the negative pressure microporous filter 151, and then the concentrated relay unit 13 and the microorganisms in the negative pressure microporous filter 151 are retained by the liquid take-up unit 19. The concentrate 15111 is taken out and collected and stored in the collection unit 20.
另外，為了維持該負壓式微孔濾材1511表面的清潔與正常的過濾功能，以避免過濾過程中微生物或其他懸浮固體阻塞住該負壓式微孔濾材1511表面的微孔，則必須透過該掃流清洗模組153以氣體噴氣、液體沖刷、化學藥劑沖泡、加溫或是超音波振盪來對負壓式微孔濾材進行清洗，以避免有阻塞的情況發生。In addition, in order to maintain the clean and normal filtering function of the surface of the negative pressure microporous filter material 1511, in order to prevent micropores or other suspended solids from blocking the micropores on the surface of the negative pressure microporous filter material 1511 during the filtration process, it is necessary to The sweep cleaning module 153 cleans the negative pressure microporous filter material by gas jet, liquid scour, chemical brewing, heating or ultrasonic oscillation to avoid clogging.
而本創作之第二實施則是正壓式，如第4A圖及第4B圖所示，與第一實施不同在於具有一正壓式微孔過濾器154，該正壓式微孔過濾器154內係具有一壓力施加模組1541及至少一個正壓式微孔濾材1542，與第一實施之負壓式微孔過濾器151不同之處，是由該壓力施加模組1541使具有微生物之原液以一定的流速通過該正壓式微孔濾材1542的表面，由於水分子在一定的壓力驅動下透過微孔濾材，而懸浮物、膠體、大分子有機物及微生物等則被阻塞於該膜上，因此具有微生物之濃縮物亦會被該正壓式微孔濾材1542阻擋，而不具有微生物的液體15422則會通過該正壓式微孔濾材1542，並由該排液模組152透過該管路17向外排出至該收集單元20中，而該收集單元20是一種被動進行收集的容器，故於容器快滿時，則能夠搬移該收集單元20，並更換一個新的收集單元20；The second implementation of the present invention is a positive pressure type, as shown in FIGS. 4A and 4B, which differs from the first embodiment in that it has a positive pressure microporous filter 154, and the positive pressure microporous filter 154 The internal system has a pressure applying module 1541 and at least one positive pressure microporous filter material 1542. The difference from the negative pressure microporous filter 151 of the first embodiment is that the pressure applying module 1541 enables the microbial stock solution. Passing through the surface of the positive pressure microporous filter material 1542 at a certain flow rate, the water molecules are driven through the microporous filter material under a certain pressure, and suspended matter, colloid, macromolecular organic matter and microorganisms are blocked on the membrane. Therefore, the microbial concentrate is also blocked by the positive pressure microporous filter material 1542, and the non-microbial liquid 15422 passes through the positive pressure microporous filter material 1542, and the liquid discharge module 152 passes through the pipeline. 17 is discharged to the collection unit 20, and the collection unit 20 is a container for passive collection, so when the container is almost full, the collection unit 20 can be moved, and a new collection unit 20 is replaced;
而該正壓式微孔濾材1542係能夠為親水性薄膜的中空纖維式過濾膜、捲式過濾膜、板式過濾膜、管式過濾膜、碟片式過濾膜、纖維性材料過濾膜或是其他任何微孔過濾材料設備所組成；The positive pressure microporous filter material 1542 can be a hollow fiber filter membrane, a roll filter membrane, a plate filter membrane, a tubular filter membrane, a disc filter membrane, a fibrous material filter membrane or the like which is a hydrophilic membrane. Any microporous filter material device;
而持續的濃縮過濾，該濃縮中繼單元13內部具有微生物之原液亦會越來越少，這一般有兩種做法，一種是將無法通過該正壓式微孔濾材1542之具有微生物的濃縮物15421回流至該濃縮中繼單元13中，以重複進行排除不具有微生物的液體15422，此種做法結果會如第4C圖所示，於停止過濾時，該正壓式微孔過濾器154內部會具有微生物的濃縮物15421，之後，則由該取液單元19將該濃縮中繼單元13與該正壓式微孔過濾器154中所具有微生物的濃縮物15111取出，並收集存放至該收集單元20中；In the case of continuous concentrated filtration, the concentration of the microorganisms in the concentration relay unit 13 is also less and less. There are generally two methods, one is that the microorganisms having the microporous filter material 1542 cannot pass through the positive pressure type microporous filter material 1542. 15421 is refluxed to the concentration relay unit 13 to repeatedly remove the liquid 15422 which does not have microorganisms. As a result, as shown in FIG. 4C, when the filtration is stopped, the positive pressure type micropore filter 154 is internally a concentrate 15421 having a microorganism, and then the concentration relay unit 13 and the concentrate 15111 having the microorganisms in the positive pressure microporous filter 154 are taken out by the liquid take-out unit 19, and collected and stored in the collection unit. 20;
另一種做法則是不將具有微生物的濃縮物回流至該濃縮中繼單元13中，而是依據該濃縮過濾單元15所能夠負擔的極限及該濃縮中繼單元13內部的液位最低點限制，持續輸送微生物之原液至該濃縮過濾單元15進行排出不具有微生物的液體15422，到該濃縮過濾單元15所能夠負擔的極限後，則不再進行過濾的動作，而於停止過濾時，則亦會於該正壓式微孔過濾器154內部保留該具有微生物的濃縮物15421，之後，則由該取液單元19將該濃縮中繼單元13與該正壓式微孔過濾器154中所具有微生物的濃縮物15111取出，並收集存放至該收集單元20中。Alternatively, the concentrate having the microorganisms is not returned to the concentration relay unit 13, but according to the limit that the concentrated filter unit 15 can bear and the minimum point limit of the liquid inside the concentration relay unit 13, The raw liquid of the microorganism is continuously transported to the concentrated filter unit 15 to discharge the liquid 15422 having no microorganisms, and after the limit that the concentrated filter unit 15 can bear, the filtering operation is not performed, and when the filtration is stopped, the liquid is also stopped. The microbial concentrate 15421 is retained inside the positive pressure microporous filter 154, and then the concentrated relay unit 13 and the positive pressure microporous filter 154 have microorganisms in the positive pressure microporous filter 154. The concentrate 15111 is taken out and collected and stored in the collection unit 20.
另外，本創作除了用於將微生物更加濃縮之外，更具有將不具有微生物的液體排出之功效，因此若是要取得不具有微生物的液體，亦能夠透過本系統達成。Further, in addition to being used to concentrate microorganisms, the present invention has an effect of discharging a liquid having no microorganisms, and therefore, if a liquid having no microorganisms is to be obtained, it can be achieved by the system.
本創作所提供之微生物培養濃縮過濾系統，與其他習用技術相互比較時，其優點如下： 1. 本創作能夠將具有微生物之原液進行過濾為具有微生物之高濃縮物、並將濃縮過濾單元中不具有微生物之液體排出，則能夠達到使培養之微生物分離出來、並濃縮微生物或收集不含微生物之液體之目的。 2. 本創作之微生物能夠於培養條件的情況下同時進行提濃，而本創作之微生物係能夠為細菌、藻類、真菌或是病毒。 3. 透過本創作之系統，將能夠於微生物培養濃縮過濾過程中節省更多的電力成本，且更能夠自動化運作，以使整體過程更容易進行操控。The microbial culture concentrated filtration system provided by this creation has the following advantages when compared with other conventional techniques: 1. This creation can filter the raw liquid with microorganisms into a high concentration of microorganisms, and will not concentrate in the filtration unit. The discharge of the liquid with microorganisms can achieve the purpose of separating the cultured microorganisms, and concentrating the microorganisms or collecting the liquid containing no microorganisms. 2. The microorganisms of this creation can be simultaneously concentrated under the conditions of culture, and the microorganisms of the present invention can be bacteria, algae, fungi or viruses. 3. Through the system of this creation, it will be able to save more electricity costs in the microbial culture and concentration filtration process, and it can be automated to make the whole process easier to handle.
本創作已透過上述之實施例揭露如上，然其並非用以限定本創作，任何熟悉此一技術領域具有通常知識者，在瞭解本創作前述的技術特徵及實施例，並在不脫離本創作之精神和範圍內，當可作些許之更動與潤飾，因此本創作之專利保護範圍須視本說明書所附之請求項所界定者為準。The present invention has been disclosed above in the above embodiments, but it is not intended to limit the present invention. Anyone skilled in the art having ordinary knowledge will understand the foregoing technical features and embodiments of the present invention without departing from the present invention. In the spirit and scope, the scope of patent protection of this creation shall be subject to the definition of the requirements attached to this manual.
1‧‧‧微生物培養濃縮過濾系統
11‧‧‧原液培養單元
12‧‧‧管路
13‧‧‧濃縮中繼單元
14‧‧‧管路
15‧‧‧濃縮過濾單元
151‧‧‧負壓式微孔過濾器
1511‧‧‧負壓式微孔濾材
15111‧‧‧具有微生物的濃縮物
15112‧‧‧不具有微生物的液體
152‧‧‧排液模組
153‧‧‧掃流清洗模組
154‧‧‧正壓式微孔過濾器
1541‧‧‧壓力施加模組
1542‧‧‧正壓式微孔濾材
15421‧‧‧具有微生物之濃縮物
15422‧‧‧不具有微生物的液體
16‧‧‧管路
17‧‧‧管路
18‧‧‧控制單元
181‧‧‧微孔阻塞監測模組
182‧‧‧過濾濾速調整模組
183‧‧‧液位保護模組
184‧‧‧溫控模組
185‧‧‧消泡模組
186‧‧‧自動清洗模組
187‧‧‧防漏監控模組
19‧‧‧取液單元
20‧‧‧收集單元1‧‧‧Microbial culture concentrated filtration system
11‧‧‧ stock solution culture unit
12‧‧‧ pipeline
13‧‧‧Concentrated relay unit
14‧‧‧ pipeline
15‧‧‧Concentrated filter unit
151‧‧‧Negative pressure microporous filter
1511‧‧‧Negative pressure microporous filter
15111‧‧‧ Concentrates with microorganisms
15112‧‧‧Liquid without microorganisms
152‧‧‧Draining module
153‧‧‧Sweep cleaning module
154‧‧‧Positive pressure microporous filter
1541‧‧‧Pressure applying module
1542‧‧‧Positive pressure microporous filter
15421‧‧‧Microbial concentrate
15422‧‧‧Liquid without microorganisms
16‧‧‧pipe
17‧‧‧pipe
18‧‧‧Control unit
181‧‧‧Microporous obstruction monitoring module
182‧‧‧Filter filter speed adjustment module
183‧‧‧ Liquid level protection module
184‧‧‧temperature control module
185‧‧‧Defoaming module
186‧‧‧Automatic cleaning module
187‧‧‧ leakproof monitoring module
19‧‧‧liquid take-up unit
20‧‧‧Collection unit
[第1圖]係本創作微生物培養濃縮過濾系統之整體架構示意圖。 [第2圖]係本創作微生物培養濃縮過濾系統之控制單元內部架構示意圖。 [第3A圖]係本創作微生物培養濃縮過濾系統之濃縮過濾單元之第一實施架構示意圖。 [第3B圖]係本創作微生物培養濃縮過濾系統之濃縮過濾單元之第一實施之濃縮過濾過程示意圖。 [第3C圖]係本創作微生物培養濃縮過濾系統之濃縮過濾單元之第一實施之濃縮過濾完成示意圖。 [第4A圖]係本創作微生物培養濃縮過濾系統之濃縮過濾單元之第二實施架構示意圖。 [第4B圖]係本創作微生物培養濃縮過濾系統之濃縮過濾單元之第二實施之濃縮過濾過程示意圖。 [第4C圖]係本創作微生物培養濃縮過濾系統之濃縮過濾單元之第二實施之濃縮過濾完成示意圖。[Fig. 1] is a schematic diagram of the overall structure of the present microbial culture concentrated filtration system. [Fig. 2] is a schematic diagram of the internal structure of the control unit of the present microbial culture concentrated filtration system. [Fig. 3A] is a schematic diagram of the first embodiment of the concentrated filtration unit of the present microbial culture concentrated filtration system. [Fig. 3B] is a schematic diagram of the concentrated filtration process of the first embodiment of the concentrated filtration unit of the present microbial culture concentrated filtration system. [Fig. 3C] is a schematic diagram of the concentrated filtration completion of the first embodiment of the concentrated filtration unit of the present microbial culture concentrated filtration system. [Fig. 4A] Fig. 4 is a schematic view showing the second embodiment of the concentrated filtration unit of the present microbial culture concentrated filtration system. [Fig. 4B] is a schematic diagram of the concentrated filtration process of the second embodiment of the concentrated filtration unit of the present microbial culture concentrated filtration system. [Fig. 4C] is a schematic diagram of the concentrated filtration completion of the second embodiment of the concentrated filtration unit of the present microbial culture concentrated filtration system.
1‧‧‧微生物培養濃縮過濾系統 1‧‧‧Microbial culture concentrated filtration system
11‧‧‧原液培養單元 11‧‧‧ stock solution culture unit
12‧‧‧管路 12‧‧‧ pipeline
13‧‧‧濃縮中繼單元 13‧‧‧Concentrated relay unit
14‧‧‧管路 14‧‧‧ pipeline
15‧‧‧濃縮過濾單元 15‧‧‧Concentrated filter unit
16‧‧‧管路 16‧‧‧pipe
17‧‧‧管路 17‧‧‧pipe
18‧‧‧控制單元 18‧‧‧Control unit
19‧‧‧取液單元 19‧‧‧liquid take-up unit
20‧‧‧收集單元 20‧‧‧Collection unit
Claims (29)
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| TW105211656U TWM538945U (en) | 2016-08-02 | 2016-08-02 | Microbial culture and enrichment and filtration system |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| TW105211656U TWM538945U (en) | 2016-08-02 | 2016-08-02 | Microbial culture and enrichment and filtration system |
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| TWM538945U true TWM538945U (en) | 2017-04-01 |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106867897A (en) * | 2017-04-19 | 2017-06-20 | 北京倍肯创新诊断技术研究院有限责任公司 | A kind of Micro biological Tests pretreating device |
| TWI648398B (en) * | 2016-08-02 | 2019-01-21 | 智水科技有限公司 | Microbial culture concentration filtration system |
-
2016
- 2016-08-02 TW TW105211656U patent/TWM538945U/en not_active IP Right Cessation
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI648398B (en) * | 2016-08-02 | 2019-01-21 | 智水科技有限公司 | Microbial culture concentration filtration system |
| CN106867897A (en) * | 2017-04-19 | 2017-06-20 | 北京倍肯创新诊断技术研究院有限责任公司 | A kind of Micro biological Tests pretreating device |
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