TWI648398B - Microbial culture concentration filtration system - Google Patents

Abstract

A microbial culture and concentration filtering system includes a dope culture unit, a concentrated relay unit, and a concentrated filtration unit, wherein the dope culture unit is a container for culturing microorganisms, and the dope culture unit has a microbial stock solution. After the raw liquid with microorganisms can enter the concentrated relay unit through a pipeline, the raw liquid with microorganisms is then transferred to the concentrated filtering unit through a pipeline, because the concentrated filtering unit can transfer the raw liquid with microorganisms The original liquid is filtered into a concentrate with microorganisms, and the liquid without microorganisms in the concentrated filtration unit is discharged, so the purpose of concentrating the cultured microorganisms can be achieved.

Description

Microbial culture concentration filtration system

The invention relates to a microbial culture concentrating and filtering system, in particular to a microbial culture and condensing filtering system which can concentrate microbial culture liquid or produce a non-microbial filter liquid.

Microbial fermentation includes liquid fermentation and solid-state fermentation. The fermentation quality of liquid fermentation is relatively stable and the fermentation speed is fast. It can be mass-produced in a short period of time. Generally, it can be divided into "one-time cultivation method" and "batch fed culture" Method "and" continuous culture method ", in which the one-time culture method is a traditional culture method in which all raw materials and microorganisms are added to the culture barrel at one time and the contents are completely taken out after the culture is completed;

The "batch fed culture method" refers to a method of intermittently or continuously supplementing fresh culture material in batches during different growth periods of microorganisms. The supplemented raw materials can be whole materials, or nitrogen sources, carbon sources, etc. The purpose is to extend the synthesis time of metabolites to achieve greater mass production or control the quality of specific products, etc .;

The "continuous culture method" refers to a fermentation method in which a new culture liquid is added to a fermentation barrel and the original culture liquid is also harvested. Its advantages are high equipment utilization, stable product quality, and convenient automatic control. The disadvantage is that it is easy to contaminate miscellaneous bacteria. Usually only suitable for strong microorganisms that have great adaptability to the environment, such as acetic acid bacteria.

In addition to the microorganisms mentioned above, some algae also belong to microorganisms. Since algae are self-supporting organisms and carbon dioxide is a carbon source for assisting growth, the more efficient the cultivation system, the more efficient the algae use carbon dioxide. . Algae culture systems can be roughly divided into two types: open systems and closed systems. The known algae open culture systems mainly include circular ponds and race-way ponds.

As the industry currently uses fully automatic fermentation tanks for liquid fermentation of microorganisms, its device contains integrated monitoring and monitoring equipment for the fermentation tank and the fermentation tank body, in order to avoid external pollution and ease of use; However, one of the biggest problems is how to separate the culture medium from the microorganisms and make the microorganisms gradually purified. In addition, centrifugation methods are generally used for processing in the past. However, this processing method can only handle a limited amount of solutions and is not efficient.

Therefore, in order to overcome the above problems, if a raw liquid having microorganisms can be filtered and a liquid having no microorganisms can be discharged through a concentration filtering unit, the cultured microorganisms can be separated and concentrated at the same time, and a microorganism-free culture can be produced at the same time. The purpose of the fluid, so should be an optimal solution.

A microbial culture concentration filtering system comprises: a stock solution culture unit, which is a container for culturing microorganisms, the stock solution culture unit is a stock solution of microorganisms; a concentrated relay unit, which communicates with the stock solution through at least one pipeline The culture unit is connected, and the raw liquid containing microorganisms in the raw liquid culture unit can enter the concentrated relay unit through the pipeline; a concentrated filtering unit is connected to the concentrated relay unit through at least one pipeline, and the The concentrated liquid containing microorganisms in the concentrated relay unit can enter the concentrated filtering unit through the pipeline, and the concentrated filtering unit is capable of filtering and discharging the liquid without microorganisms, so as to retain the concentrate with higher concentration of microorganisms.

More specifically, the concentrated filtration unit has at least one negative pressure microporous filter and a drainage module connected to the negative pressure microporous filter. The negative pressure microporous filter passes through at least A pipeline is connected to the concentrated relay unit, and the raw liquid containing microorganisms in the concentrated relay unit can enter the negative pressure microporous filter through the pipeline. The negative pressure microporous filter has At least one negative pressure microporous filter material, and when the raw liquid with microorganisms passes through the negative pressure microporous filter material, the concentrate with microorganisms will be blocked by the negative pressure microporous filter material, and the drainage module is The non-microbe-free liquid that can pass through the negative pressure type microporous filter material is discharged.

More specifically, the negative-pressure microporous filter can return the raw liquid with microorganisms blocked by the negative-pressure microporous filter material to the concentrated relay unit, and repeatedly reflux and circulate and filter out the non-microorganic filter. It is a liquid and can retain a concentrate with a higher concentration of microorganisms in the concentration relay unit and the negative-pressure microporous filter.

More specifically, the raw liquid with microorganisms inside the concentrating relay unit continuously enters the negative pressure micro-pore filter to exclude the liquid without microorganisms, while retaining a higher level in the negative pressure micro-pore filter. Concentrate of microorganisms.

More specifically, the microbial culture and concentration filtration system further includes at least one liquid extraction unit and at least one collection unit, wherein the liquid extraction unit can be used in the concentration relay unit and the negative pressure microporous filter. The concentrate with a higher concentration of microorganisms is taken out and stored in the collection unit, and the drainage module is more capable of discharging and storing liquids without microorganisms in another collection unit.

More specifically, the negative pressure type microporous filter material can be a hollow fiber filter membrane, a roll filter membrane, a plate filter membrane, a tube filter membrane, a disc filter membrane, or any other type of membrane that can be a hydrophilic film. Microporous filter material blocking microbes.

More specifically, the condensing and filtering unit further has a sweep cleaning module, which can clean the negative pressure microporous filter material by gas jet, liquid scouring, chemical brewing, heating or ultrasonic vibration. It is used to maintain the cleanliness and normal filtering function of the surface of the negative pressure microporous filter material, so as to prevent microorganisms from blocking the micropores on the surface of the negative pressure microporous filter material during the filtering process.

More specifically, the microbial culture and concentration filtering system further includes a control unit connected to the concentration relay unit and the concentration filter unit, for controlling the filtering operation status of the concentration relay unit and the concentration filter unit. .

More specifically, the control unit further has a micropore blocking monitoring module for detecting the pressure and flow rate of the microbe-containing stock solution passing through the negative pressure type micropore filter material to determine the degree of micropore blocking.

More specifically, the control unit further includes a filtering speed adjustment module for detecting the filtering pressure, the flow rate or the concentration of the concentrate with microorganisms when passing through the negative pressure microporous filter medium. Concentration as a reference for adjusting the filtration speed.

More specifically, there is a liquid level protection module in the control unit, which is used to detect the liquid water level inside the concentrating relay unit and the negative pressure microporous filter, and the liquid water level is used for It can be judged whether the concentrated relay unit should continue to transport the microbial-containing raw liquid into the negative pressure micro-pore filter for filtering out the liquid without microorganisms.

More specifically, the control unit further has a temperature control module for detecting and controlling the temperature of the liquid inside the concentrating relay unit and the negative-pressure microporous filter.

More specifically, there is a defoaming module in the control unit, which is used to eliminate bubbles formed in the liquid inside the concentrating relay unit and the negative pressure microporous filter due to liquid transportation or sweeping. .

More specifically, the control unit further has an automatic cleaning module for automatically cleaning the inside of the concentrated relay unit and the negative pressure when the concentrated relay unit and the concentrated filtering unit are not in operation. Inside of a pressure microporous filter.

More specifically, the control unit further has a leak-proof monitoring module for monitoring the state of the liquid discharged by the drainage module to prevent the concentrate with microorganisms from being completely damaged by the damage of the microporous filter material. The negative pressure type microporous filter material is blocked, which causes the liquid discharge module to discharge liquid containing microorganisms.

More specifically, the concentrated filtration unit includes a positive pressure microporous filter and a drainage module connected to the positive pressure microporous filter. The positive pressure microporous filter passes through at least one The pipeline is connected to the concentrated relay unit, and the positive pressure microporous filter has a pressure application module and at least one positive pressure microporous filter material. The concentrated liquid containing microorganisms can pass through The pipeline is connected to the pressure application module of the positive pressure microporous filter, so that when the raw liquid with microorganisms is driven through the positive pressure microporous filter medium under pressure, the concentrate with microorganisms will be pressured by the positive pressure. Type microporous filter material is blocked, and the drainage module discharges the positive pressure type microporous filter material without blocking the liquid without microorganisms.

More specifically, the positive-pressure microporous filter can return the raw liquid with microorganisms blocked by the positive-pressure microporous filter material to the concentrated relay unit, and repeatedly reflux and circulate and filter out the non-microbial The liquid is capable of retaining a concentrate having a higher concentration of microorganisms in the concentrated relay unit.

More specifically, if the raw liquid with microorganisms inside the concentrated relay unit continues to enter the positive pressure microporous filter to exclude the liquid without microorganisms, it can be retained in the positive pressure microporous filter. Concentrate of high concentration microorganisms.

More specifically, the microbial culture and concentration filtration system further includes at least one liquid extraction unit and at least one collection unit, wherein the liquid extraction unit can be used in the concentration relay unit and the negative pressure microporous filter. The concentrate with a higher concentration of microorganisms is taken out and stored in the collection unit, and the drainage module is more capable of discharging and storing liquids without microorganisms in another collection unit.

More specifically, the positive pressure type microporous filter material can be a hollow fiber filter membrane, a roll filter membrane, a plate filter membrane, a tube filter membrane, a disc filter membrane, or a fibrous material, which can be a hydrophilic film. Composed of filter membranes.

More specifically, the condensing and filtering unit further has a sweep cleaning module, which can clean the positive pressure microporous filter material by gas jet, liquid scouring, chemical brewing, heating or ultrasonic oscillation. It is used to maintain the cleanness and normal filtering function of the surface of the positive pressure type microporous filter material, so as to prevent microorganisms from blocking the micropores on the surface of the positive pressure type microporous filter material during the filtering process.

More specifically, the microbial culture and concentration filtering system further includes a control unit connected to the concentration relay unit and the concentration filter unit, for controlling the filtering operation status of the concentration relay unit and the concentration filter unit. .

More specifically, the control unit further has a micropore blocking monitoring module for detecting the pressure and flow rate of the microbial-containing stock solution passing through the positive-pressure micropore filter medium to determine the degree of micropore blocking.

More specifically, the control unit further includes a filtering speed adjustment module for detecting the filtering pressure, the flow rate or the concentration of the concentrate containing microorganisms when passing through the positive pressure microporous filter medium. Concentration as a reference for adjusting the filtration speed.

More specifically, there is a liquid level protection module in the control unit for detecting the height of the liquid water level inside the concentrating relay unit, and the liquid level is used to determine whether the concentrating relay unit is required. Continue to transport the raw liquid of microorganisms into the positive pressure micro-pore filter for filtering out liquids without microorganisms.

More specifically, the control unit further has a temperature control module for detecting and controlling the temperature of the liquid inside the concentrating relay unit.

More specifically, the control unit further has a defoaming module to eliminate bubbles formed in the liquid inside the concentrated relay unit due to liquid transportation or sweeping.

More specifically, the control unit further includes an automatic cleaning module for automatically cleaning the inside of the concentrated relay unit or the concentrated filtering unit when the concentrated relay unit or the concentrated filtering unit is not in operation. .

More specifically, there is a leak-proof monitoring module in the control unit, which is used to monitor the state of the liquid discharged by the liquid discharge module, so as to avoid that the concentrate with microorganisms cannot be completely evacuated by the positive pressure type. The pore filter material hinders the liquid discharge module from discharging liquid containing microorganisms.

Regarding other technical contents, features and effects of the present invention, they will be clearly presented in the following detailed description of the preferred embodiments with reference to the drawings.

Please refer to FIG. 1 and FIG. 2, which are a schematic diagram of the overall architecture of a microbial culture and concentration filtration system and a schematic diagram of the internal structure of a control unit of the present invention. A concentration relay unit 13, a concentration filtration unit 15, a control unit 18, a liquid extraction module 19 and a collection module 20, and the original liquid culture unit 11, the concentration relay unit 13, the concentration filtration unit 15, the control Liquid is transported between the units 18 through the pipelines 12, 13, 14, 16, 17;

The stock solution culture unit 11 is a container for culturing microorganisms, and the stock solution culture unit is a stock solution of microorganisms; wherein the concentrated relay unit 13 is connected to the stock solution culture unit 11 through the pipeline 12 to So that the raw liquid containing microorganisms in the raw liquid culture unit 11 can enter the concentrated relay unit 13 through the pipeline 12;

The condensing and filtering unit 15 is connected to the concentrating and relaying unit 13 through the pipeline 14 so that the raw liquid containing microorganisms in the concentrating and relaying unit 13 can enter the condensing and filtering unit 15 through the pipeline 14. Since the concentrated filtering unit 15 has at least one set of filtering materials inside, the concentrated filtering unit 15 can filter the liquid without microorganisms and discharge it through the pipeline 17, and retain the concentrate with a higher concentration of microorganisms. The higher concentration of microorganisms can be discharged back to the concentrated relay unit 13 through the pipeline 16, and the repeated filtration and reflux procedures can make the concentration of the microorganisms contained in the raw liquid in the concentrated relay unit 13 increase. The higher.

The control unit 18 is used to control the filtering operation status of the concentrating relay unit 13 and the condensing filtering unit 15. The control unit 18 includes a microporous obstruction monitoring module 181 and a filtering and filtering speed adjusting module 182. , A liquid level protection module 183, a temperature control module 184, a defoaming module 185, an automatic cleaning module 186 and a leak-proof monitoring module 187;

The micropore clogging monitoring module 181 is used to detect the pressure and flow rate of the raw liquid with microorganisms when passing through the filter material in the concentrated filtering unit 15 to determine the degree of micropore clogging, and the filtering speed adjustment module 182 is It is used to detect the filtration pressure, the flow rate during filtration, or the concentration of the concentrate when the concentrate with microorganisms passes through the filter material in the concentrated filtration unit 15 as a reference basis for adjusting the filtration speed;

The liquid level protection module 183 is used to detect the liquid water level height, and the liquid water level height can determine whether the concentration relay unit 13 should continue to transport the original liquid with microorganisms into the concentration filtration unit 15 for filtering out. Treatment of liquids of microorganisms; the liquid level protection module 183 can also determine the liquid level of the concentration filter unit 15 to protect the microporous filter material from damage due to the liquid level being too low or decide to stop the discharge of the concentrate action;

The temperature control module 184 is mainly used to detect and control the liquid temperature in the concentrated relay unit 13 and the concentrated filter unit 15; in addition, the defoaming module 185 is used to eliminate the internal temperature of the concentrated relay unit 13 Bubbles formed during liquid transfer due to liquid and bubbles formed during liquid transfer or cleaning sweep in the concentrated filtering unit 15;

The automatic cleaning module 185 is used to automatically clean the inside of the concentration relay unit 13 and the filter material and container of the concentration filter unit 15 when the concentration relay unit 13 and the concentration filter unit 15 are not in operation. And pipes. In addition, in order to prevent the concentrate with microorganisms from being completely blocked by the filtering material inside the concentration filtering unit 15 and causing the discharge of the liquid with microorganisms, it is necessary to monitor the state of the discharged liquid through the leak-proof monitoring module 187. If the turbidity is too high, it is judged that the filtering material inside the condensing filtering unit 15 may have broken holes or other problems that cause filtering failure.

The condensing and filtering unit 15 can be of two types. The first is a negative pressure type, and the second is a positive pressure type. The first embodiment of the present invention is a negative pressure type, as shown in FIG. 3A and FIG. 3B. As shown in the figure, the concentrated filtering unit 15 has a negative pressure microporous filter 151, a liquid discharge module 152, and a sweep cleaning module 153 inside. The concentrated liquid containing microorganisms in the concentrated relay unit 13 can pass through. The pipeline 15 enters the negative pressure microporous filter 151. As shown in FIG. 3B, the negative pressure microporous filter 151 includes a liquid container, and more than one group is placed in the liquid container. Of the negative pressure microporous filter material 1511, and after the raw liquid with microorganisms enters the negative pressure microporous filter 151, due to the role of the drainage module 152, it will pass through the negative pressure microporous filter material 1511, but it has the part of microorganisms It cannot pass through the negative pressure type microporous filter material 1511, but stays in the negative pressure type microporous filter material 151, and the liquid 15112 without microorganisms passes through the negative pressure type microporous filter material 1511 and is discharged by the row The liquid module 152 is discharged to the collection unit 20 through the pipeline 17, and 20 is a passive collection unit for collecting container, so the container is almost full at the time, it is possible to move the collecting unit 20, and replaced with a new collection unit 20;

The negative pressure type microporous filter material 1511 is a hollow fiber filter membrane, a roll filter membrane, a plate filter membrane, a tube filter membrane, a disc filter membrane, or any other microporous membrane that can be a hydrophilic film. Composed of filter media;

With continuous concentration filtration, the stock solution containing microorganisms in the concentration relay unit 13 will become less and less. There are generally two methods. One is to pass the negative pressure type microporous filter material 1511 and the microorganism-containing concentrate 15111. Return to the concentrated relay unit 13 to repeatedly remove the liquid 15112 without microorganisms. The result of this approach is shown in Figure 3C. When the filtration is stopped, the concentrated relay unit 13 and the negative pressure The pore filter 151 will have a microbial condensate 15111 inside, and then, the liquid collecting unit 19 will take the concentrating relay unit 13 and the negative pressure microporous filter 151 with the microbial condensate 15111, and Collected and stored in the collection unit 20;

Another approach is not to return the concentrate with microorganisms to the concentrated relay unit 13, but to limit it according to the limit afforded by the concentrated filter unit 15 and the lowest point of the liquid level inside the concentrated relay unit 13, Continuously transporting the raw liquid of microorganisms to the concentrated filtration unit 15 to discharge the liquid 15112 without microorganisms. After reaching the limit that the concentrated filtration unit 15 can afford, the filtering operation will no longer be performed. When the filtration is stopped, only The negative pressure microporous filter 151 retains the microbe-concentrated concentrate 15111 inside, and then, the liquid collecting unit 19 and the concentrated relay unit 13 and the negative pressure microporous filter 151 have microorganisms. The concentrate 15111 is taken out and collected and stored in the collection unit 20.

In addition, in order to maintain the cleanliness and normal filtering function of the surface of the negative pressure microporous filter material 1511, to prevent microorganisms or other suspended solids from blocking the micropores on the surface of the negative pressure microporous filter material 1511 during the filtration process, it is necessary to penetrate the The sweep cleaning module 153 cleans the negative pressure type microporous filter material by using gas jet, liquid scouring, chemical brewing, heating or ultrasonic vibration to avoid blocking.

The second embodiment of the present invention is a positive pressure type, as shown in FIG. 4A and FIG. 4B, which is different from the first embodiment in that it has a positive pressure microporous filter 154, which is a positive pressure microporous filter 154 The internal system has a pressure application module 1541 and at least one positive pressure type microporous filter material 1542, which is different from the negative pressure type microporous filter 151 of the first embodiment in that the pressure application module 1541 makes the original liquid with microorganism At a certain flow rate, it passes through the surface of the positive pressure microporous filter medium 1542. As water molecules are driven through the microporous filter medium under a certain pressure, suspended matter, colloids, macromolecular organic matter and microorganisms are blocked on the membrane. Therefore, the concentrate with microorganisms will also be blocked by the positive pressure microporous filter material 1542, and the liquid 15422 without microorganisms will pass through the positive pressure microporous filter material 1542 and pass through the pipe by the drainage module 152 17 is discharged outward into the collection unit 20, and the collection unit 20 is a passive collection container, so when the container is almost full, the collection unit 20 can be moved and a new collection unit 20 can be replaced;

The positive pressure type microporous filter material 1542 can be a hollow fiber filter membrane, a roll filter membrane, a plate filter membrane, a tube filter membrane, a disc filter membrane, a fibrous material filter membrane, or other hydrophilic membranes. Consisting of any microporous filter material equipment;

With continuous concentration filtration, the stock solution containing microorganisms inside the concentration relay unit 13 will also become less and less. There are generally two methods. One is to pass the positive pressure microporous filter material 1542 with the microorganism concentration. 15421 is returned to the concentrated relay unit 13 to repeatedly remove the liquid without microbes 15422. The result of this method will be shown in Figure 4C. When the filtration is stopped, the positive pressure microporous filter 154 will The microorganism-containing concentrate 15421 is then taken out by the liquid taking unit 19 to the concentration relay unit 13 and the microorganism-containing concentrate 15111 in the positive-pressure microfiltration filter 154, and collected and stored in the collection unit 20 in

Another approach is not to return the concentrate with microorganisms to the concentrated relay unit 13, but to limit it according to the limit afforded by the concentrated filter unit 15 and the lowest point of the liquid level inside the concentrated relay unit 13, Continuously transport the raw liquid of microorganisms to the concentrated filtering unit 15 to discharge the liquid 15422 without microorganisms. After reaching the limit that the concentrated filtering unit 15 can afford, the filtering operation will no longer be performed, and when the filtering is stopped, it will also The microorganism-containing concentrate 15421 is retained inside the positive-pressure micro-filter 154, and then, the liquid-receiving unit 19, the concentration relay unit 13 and the microorganisms in the positive-pressure micro-filter 154 are retained The concentrate 15111 is taken out and collected and stored in the collection unit 20.

In addition, the present invention has the effect of discharging microorganism-free liquids in addition to more concentrated microorganisms. Therefore, if a liquid without microorganisms is to be obtained, it can also be achieved through the system.

When compared with other conventional technologies, the microbial culture and concentration filtration system provided by the present invention has the following advantages: (1) The present invention can filter the raw liquid with microorganisms into a high concentration with microorganisms, and the concentrated filtration unit The discharge of liquids without microorganisms can achieve the purpose of separating the cultured microorganisms and concentrating the microorganisms or collecting liquids without microorganisms. (2) The microorganisms of the present invention can be concentrated at the same time under the conditions of culture, and the microorganisms of the present invention can be bacteria, algae, fungi or viruses. (3) Through the system of the present invention, it will be able to save more power costs in the process of microbial culture concentration and filtration, and will be able to operate more automatically, so that the overall process is easier to control.

The present invention has been disclosed as above through the above-mentioned embodiments, but it is not intended to limit the present invention. Anyone who has ordinary knowledge in this technical field will hope to describe the present invention more clearly through the detailed description of the preferred embodiments above. The features and spirit of the invention do not limit the scope of the invention with the preferred embodiments disclosed above. On the contrary, the intention is to cover various changes and equivalent arrangements within the scope of the patents to be applied for in the present invention.

1‧‧‧ Microbial Culture Concentration Filtration System

11‧‧‧ dope culture unit

12‧‧‧ pipeline

13‧‧‧Concentrated relay unit

14‧‧‧pipe

15‧‧‧Concentrated filter unit

151‧‧‧Negative pressure microporous filter

1511‧‧‧Negative pressure microporous filter

15111‧‧‧ Concentrate with microorganisms

15112‧‧‧Liquids without microorganisms

152‧‧‧Draining module

153‧‧‧Sweep cleaning module

154‧‧‧Positive pressure microporous filter

1541‧‧‧Pressure Application Module

1542‧‧‧Positive Pressure Microporous Filter Media

15421‧‧‧ Concentrate with microorganisms

15422‧‧‧Liquids without microorganisms

16‧‧‧ pipeline

17‧‧‧ pipeline

18‧‧‧Control unit

181‧‧‧Microporous blockage monitoring module

182‧‧‧Filter filter speed adjustment module

183‧‧‧Liquid level protection module

184‧‧‧Temperature Control Module

185‧‧‧defoaming module

186‧‧‧Automatic cleaning module

187‧‧‧Leakproof monitoring module

19‧‧‧ Liquid taking unit

20‧‧‧ Collection Unit

[FIG. 1] It is a schematic diagram of the overall structure of the microbial culture and concentration filtration system of the present invention. [Fig. 2] It is a schematic diagram of the internal structure of the control unit of the microorganism culture and concentration filtration system of the present invention. [FIG. 3A] It is a schematic diagram of the first implementation structure of the concentration and filtration unit of the microbial culture and concentration and filtration system of the present invention. [Fig. 3B] It is a schematic diagram of the first embodiment of the concentrated filtration process of the concentrated filtration unit of the microorganism culture and concentrated filtration system of the present invention. [FIG. 3C] It is a schematic diagram of the completion of the concentrated filtration of the first implementation of the concentrated filtration unit of the microorganism culture and concentrated filtration system of the present invention. [Fig. 4A] It is a schematic diagram of the second implementation structure of the concentrating and filtering unit of the microbial culture and concentrating and filtering system of the present invention. [Fig. 4B] It is a schematic diagram of the second embodiment of the concentrated filtration process of the concentrated filtration unit of the microorganism culture and concentrated filtration system of the present invention. [FIG. 4C] A schematic diagram of the completion of the concentrated filtration of the second implementation of the concentrated filtration unit of the microorganism culture and concentrated filtration system of the present invention.

Claims (29)

A microbial culture concentration filtering system comprises: a stock solution culture unit, which is a container for culturing microorganisms, the stock solution culture unit is a stock solution of microorganisms; a concentrated relay unit, which communicates with the stock solution through at least one pipeline The culture unit is connected, and the raw liquid containing microorganisms in the raw liquid culture unit can enter the concentrated relay unit through the pipeline; a concentrated filtering unit is connected to the concentrated relay unit through at least one pipeline, and the The concentrated liquid with microorganisms in the concentrated relay unit can enter the concentrated filtering unit through the pipeline, and the concentrated filtering unit can filter and discharge the liquid without microorganisms, so as to retain the concentrate with higher concentration of microorganisms, And the raw liquid with microorganisms blocked by the concentrated filtering unit can be returned to the concentrated relay unit, and the liquid with microorganisms can be repeatedly refluxed and circulated to filter out the microorganisms with a higher concentration in the concentrated relay unit. Of concentrates. The microbial culture and concentration filtration system according to claim 1, wherein the concentration filtration unit has at least a negative pressure micropore filter and a drainage module connected to the negative pressure micropore filter, and the negative pressure The microporous filter is connected to the concentrated relay unit through at least one pipeline, and the raw liquid containing microorganisms in the concentrated relay unit can enter the negative pressure micropore filter through the pipeline. The negative pressure In the microporous filter, there is at least one negative pressure microporous filter material, and when the raw liquid with microorganisms passes through the negative pressure microporous filter material, the concentrate with microorganisms will be blocked by the negative pressure microporous filter material. , And the liquid discharge module will discharge the liquid without microorganisms which can be blocked by the negative pressure microporous filter material. The microbial culture and concentration filtration system according to claim 2, wherein the negative pressure microporous filter can return the original liquid with microorganisms blocked by the negative pressure microporous filter material to the concentrated relay unit, and repeat the reflux And the liquid which does not have microorganisms is circulated and filtered, and a concentrate with a higher concentration of microorganisms can be retained in the concentration relay unit and the negative-pressure microporous filter. The microbial culture and concentration filtering system according to claim 2, wherein the raw liquid with microorganisms inside the concentration relay unit continuously enters the negative pressure micro-pore filter to exclude the liquid without microorganisms, and at the same time Concentrates with higher concentrations of microorganisms remain in the pore filter. The microbial culture and concentration filtration system according to claim 3 or 4, further comprising at least one liquid extraction unit and at least one collection unit, wherein the liquid extraction unit is capable of filtering the concentration relay unit and the negative pressure micropore filter The concentrate with a higher concentration of microorganisms in the device is taken out and stored in the collection unit, and the liquid discharge module is more capable of discharging and storing liquids without microorganisms in another collection unit. The microbial culture and concentration filtration system according to claim 2, wherein the negative pressure microporous filter material is a hollow fiber filter membrane, a roll filter membrane, a plate filter membrane, a tube filter membrane, or a dish capable of being a hydrophilic film. It consists of a sheet filter membrane. The microbial culture and concentration filtration system according to claim 2, wherein the concentration filtration unit further has a sweeping and cleaning module, which can be counteracted by gas jet, liquid scouring, chemical brewing, heating or ultrasonic vibration. The pressure type microporous filter material is cleaned to maintain the surface of the negative pressure microporous filter material clean and normal filtering function, so as to prevent microorganisms from blocking the micropores on the surface of the negative pressure microporous filter material during the filtering process. The microbial culture and concentration filtering system according to claim 2, further comprising a control unit connected to the concentration relay unit and the concentration filtering unit, for controlling the filtering operation status of the concentration relay unit and the concentration filtering unit. . The microbial culture and concentration filtration system according to claim 8, wherein the control unit further has a micropore blocking monitoring module for detecting the pressure and flow rate of the raw liquid with microorganisms passing through the negative pressure micropore filter material, To determine the degree of micropore blockage. The microbial culture and concentration filtration system according to claim 8, wherein the control unit further has a filtration speed adjustment module for detecting the filtration pressure when the concentrate with microorganisms passes through the negative pressure microporous filter medium, The flow rate or the concentration of the concentrate during filtration is used as a reference for adjusting the filtration speed. The microbial culture and concentration filtering system according to claim 8, wherein the control unit further includes a liquid level protection module for detecting the height of the liquid water level in the concentration relay unit and the negative pressure microporous filter. The height of the liquid water level can be used to determine whether the concentrated relay unit should continue to transport the raw liquid of microorganisms into the negative pressure micro-pore filter for filtering out liquids without microorganisms. The microbial culture and concentration filtering system according to claim 8, wherein the control unit further has a temperature control module for detecting and controlling the temperature of the liquid inside the concentration relay unit and the negative-pressure microporous filter. . The microbial culture concentrating and filtering system according to claim 8, wherein the control unit further has a defoaming module to eliminate the liquid in the concentrating relay unit and the negative-pressure microporous filter due to liquid transport or Air bubbles formed during sweeping. The microorganism culture and concentration filtering system according to claim 8, wherein the control unit further has an automatic cleaning module for automatically cleaning the concentration relay unit or the concentration filtering unit when the concentration filtering unit is not in operation. Inside the concentrating relay unit or inside the negative-pressure microporous filter. The microbial culture and concentration filtration system according to claim 8, wherein the control unit further has a leak-proof monitoring module for monitoring the state of the liquid discharged by the drainage module to avoid the microorganism-containing concentrate Because the microporous filter material is damaged and cannot be completely blocked by the negative pressure microporous filter material, the liquid discharge module discharges liquid containing microorganisms. The microbial culture and concentration filtration system according to claim 1, wherein the concentration filtration unit includes a positive pressure microporous filter and a drainage module connected to the positive pressure microporous filter, the positive pressure type The micropore filter is connected to the concentrating relay unit through at least one pipeline, and the positive pressure micropore filter has a pressure application module and at least one positive pressure micropore filter material inside the concentrating relay. The stock solution with microorganisms in the unit can be connected to the pressure application module of the positive pressure microporous filter through the pipeline, so that when the stock solution with microorganisms is driven under pressure through the positive pressure microporous filter medium, the stock solution with microorganisms The concentrate will be blocked by the positive pressure microporous filter material, and the drainage module will discharge the non-microorganic liquid that the positive pressure microporous filter material does not block. The microbial culture and concentration filtration system according to claim 16, wherein the positive pressure type microporous filter can return the original liquid with microorganisms blocked by the positive pressure type microporous filter material to the concentrated relay unit and repeat the return flow If the liquid with microorganisms is filtered out, the concentrate with higher concentration of microorganisms can be retained in the concentrated relay unit. The microbial culture and concentration filtering system according to claim 16, wherein the raw liquid with microorganisms inside the concentrating relay unit continues to enter the positive pressure type microporous filter to exclude liquids without microorganisms, and the positive pressure type Concentrates with higher concentrations of microorganisms remain in the microporous filters. The microbial culture and concentration filtration system according to claim 17 or 18, further comprising at least one liquid extraction unit and at least one collection unit, wherein the liquid extraction unit is capable of the concentration relay unit and the negative-pressure microporous filter The concentrate with a higher concentration of microorganisms is taken out and stored in the collection unit, and the drainage module is more capable of discharging and storing liquids without microorganisms in another collection unit. The microbial culture and concentration filtration system according to claim 16, wherein the positive pressure type microporous filter material is a hollow fiber filter membrane, a roll filter membrane, a plate filter membrane, a tube filter membrane, and a disc capable of being a hydrophilic film. Filter membrane or fibrous material filter membrane. The microbial culture and concentration filtration system according to claim 16, wherein the concentration filtration unit further includes a sweeping and cleaning module, which can be aligned by gas jets, liquid scouring, chemical brewing, heating, or ultrasonic vibration. The pressure type microporous filter material is cleaned to maintain the surface of the positive pressure type microporous filter material to be clean and normal filtering function, so as to prevent microorganisms from blocking the micropores on the surface of the positive pressure type microporous filter material during the filtering process. The microbial culture and concentration filtering system according to claim 16, further comprising a control unit connected to the concentration relay unit and the concentration filtering unit, for controlling the filtering operation status of the concentration relay unit and the concentration filtering unit. . The microbial culture and concentration filtration system according to claim 22, wherein the control unit further has a micropore blocking monitoring module for detecting the pressure and flow rate of the raw liquid with microorganisms passing through the positive pressure micropore filter material, To determine the degree of micropore blockage. The microbial culture and concentration filtration system according to claim 22, wherein the control unit further has a filtration speed adjustment module for detecting the filtration pressure when the concentrate with microorganisms passes through the positive-pressure microporous filter medium, The flow rate or the concentration of the concentrate during filtration is used as a reference for adjusting the filtration speed. The microbial culture and concentration filtering system according to claim 22, wherein the control unit further has a liquid level protection module for detecting a liquid water level inside the concentration relay unit, and the liquid water level is used to be able to It is judged whether the concentrated relay unit should continue to transport the raw liquid of microorganisms into the positive-pressure micropore filter for filtering out liquids without microorganisms. The microbial culture and concentration filtering system according to claim 22, wherein the control unit further has a temperature control module for detecting and controlling the temperature of the liquid inside the concentration relay unit. The microbial culture concentrating and filtering system according to claim 22, wherein the control unit further has a defoaming module for removing bubbles formed in the liquid inside the concentrating relay unit due to liquid transportation or sweeping. The microbial culture and concentration filtering system according to claim 22, wherein the control unit further has an automatic cleaning module for automatically cleaning the concentration when the concentration relay unit or the concentration filtering unit is not in operation. Inside the relay unit or concentrating filter unit. The microbial culture and concentration filtration system according to claim 22, wherein the control unit further has a leak-proof monitoring module for monitoring the state of the liquid discharged by the drainage module to avoid the microorganism-containing concentrate Cannot be completely blocked by the positive pressure type microporous filter material, which causes the liquid discharge module to discharge liquid containing microorganisms.