CN213388689U

CN213388689U - Fixed bed bioreactor

Info

Publication number: CN213388689U
Application number: CN202021812762.9U
Authority: CN
Inventors: 梁智杰; 崔利凯; 李高军
Original assignee: Boaovax Biotechnology Co ltd
Current assignee: Boaovax Biotechnology Co ltd
Priority date: 2020-08-26
Filing date: 2020-08-26
Publication date: 2021-06-08
Anticipated expiration: 2030-08-26

Abstract

The utility model discloses a fixed bed bioreactor. The fixed bed bioreactor comprises: the harvesting port is a through hole formed in the side wall of the reactor; one end of the main pipe extends into the bottom of the reactor, and the other end of the main pipe extends out of the reactor after penetrating through the harvesting port; one end of the first branch pipe is connected with the header pipe, and the other end of the first branch pipe is connected with the sampling device; one end of the second branch pipe is connected with the header pipe, and the other end of the second branch pipe is connected with the harvesting device; the valve assembly comprises a three-way valve arranged at the joint of the first branch pipe, the second branch pipe and the main pipe and a main valve arranged on the main pipe, or comprises a first valve arranged on the first branch pipe, a second valve arranged on the second branch pipe and a main valve arranged on the main pipe. Firstly, no sampling port is arranged, so that the tank body strength of the bioreactor can be improved, and the construction difficulty of the sampling port is avoided. Secondly, since the sampling port is not provided any more, the sterilization operation can be reduced.

Description

Fixed bed bioreactor

Technical Field

The utility model belongs to the technical field of fixed bed bioreactor, particularly, relate to fixed bed bioreactor of results and sample integration.

Background

Bioreactors are increasingly used for their large-scale production of microorganisms. During the culture of microorganisms such as cells and viruses, it is necessary to continuously sample and observe the growth state or culture product. For this reason, the bioreactor is usually designed to have a sampling port in addition to the harvesting port, and such a sampling method has the following problems:

1) the strength of the bioreactor tank body is obviously reduced due to the arrangement of the sampling port; the construction difficulty for arranging the sampling port is high;

2) the tank wall of the bioreactor is also provided with a jacket which passes through the jacket to connect the sampling valve with the tank wall, so that the construction difficulty is complex.

3) The sampling valve needs to be sterilized separately, and the operation is increased.

4) The sampling valve and the tank wall are sealed by an O-shaped rubber ring, and the risk of incomplete sterilization exists in the sterilization process.

5) The sample can remain in sample valve and the pipeline after the sample is accomplished, gets rid of the difficulty, is unfavorable for the accuracy of next sample.

SUMMERY OF THE UTILITY MODEL

The utility model discloses a main aim at provides fixed bed bioreactor to solve the jar body intensity that exists among the prior art poor, the sampling device construction degree of difficulty is high, the technical problem that the sample valve need sterilize alone.

In order to solve the technical problem, the utility model provides a fixed bed bioreactor. The fixed bed bioreactor comprises:

the harvesting port is a through hole formed in the side wall of the reactor;

one end of the main pipe extends into the bottom of the reactor, and the other end of the main pipe extends out of the reactor after penetrating through the harvesting port;

one end of the first branch pipe is connected with the header pipe, and the other end of the first branch pipe is connected with the sampling device;

one end of the second branch pipe is connected with the header pipe, and the other end of the second branch pipe is connected with the harvesting device;

the valve assembly comprises a three-way valve arranged at the joint of the first branch pipe, the second branch pipe and the main pipe and a main valve arranged on the main pipe, or comprises a first valve arranged on the first branch pipe, a second valve arranged on the second branch pipe and a main valve arranged on the main pipe.

Firstly, no sampling port is arranged, so that the tank body strength of the bioreactor can be improved, and the construction difficulty of the sampling port is avoided. Secondly, since the sampling port is not provided any more, the sterilization operation can be reduced. It can be seen that, the utility model discloses a fixed bed bioreactor rational utilization results mouth all takes a sample and results through the results mouth, has simplified bioreactor's tank structure, has promoted intensity.

Further, the main valve is a differential pressure control valve. Thus, when the pressure difference across the main valve reaches a preset value, the main valve opens, otherwise the main valve closes.

Furthermore, sampling device includes sampling flask, sampling tube and first exhaust tube, sampling tube one end with first leg joint, inside the other end passed the sampling flask body and stretched into the sampling flask, inside first exhaust tube one end passed the sampling flask body and stretched into the sampling flask, the other end and air exhaust device are connected, be equipped with first bleeder valve on the first exhaust tube. From this, when needing the sample, open first bleeder valve and air exhaust device and bleed the sampling bottle and open the main valve when reaching the pressure differential default of main valve, liquid in the reactor flows into the sampling bottle, after the sample, closes air exhaust device, and the pressure differential of main valve both sides diminishes, and the main valve is closed, and liquid in the main pipe flows back to in the bioreactor.

Furthermore, the sampling device comprises a bottle cap in threaded connection with the sampling bottle, and the sampling tube and the first extraction tube penetrate through the bottle cap and then extend into the sampling bottle. Therefore, only the sampling bottle needs to be replaced when sampling at every time, and the operation is reduced.

Further, the device also comprises a purging device for cleaning the first branch pipe after sampling. Therefore, the accuracy of the next sampling detection result can be improved.

Furthermore, the blowing device comprises a compressed air conveying pipe, the compressed air conveying pipe is connected with the first branch pipe, and an air inlet valve is arranged on the compressed air conveying pipe. From this, sweep first branch pipe through compressed air, it is efficient and clean effectual.

Further, the purging device further comprises a heater, and the heater heats the compressed air in the compressed air conveying pipe. Thereby, the cleaning speed and the cleaning effect can be further improved by using the hot compressed air.

Furthermore, the harvesting device comprises a harvesting bottle, a harvesting pipe and a second air exhaust pipe, one end of the harvesting pipe is connected with the second branch pipe, the other end of the harvesting pipe penetrates through the body of the harvesting bottle and then extends into the harvesting bottle, one end of the second air exhaust pipe penetrates through the body of the harvesting bottle and then extends into the harvesting bottle, the other end of the second air exhaust pipe is connected with the air exhaust device, and a second air exhaust valve is arranged on the second air exhaust pipe. Therefore, when harvesting is needed, the second air extraction valve and the air extraction device are opened to extract air to the harvesting bottle, the main valve is opened when the pressure difference preset value of the main valve is reached, and liquid in the reactor flows into the harvesting bottle.

Further, an exhaust pipeline is arranged on the reactor, and an exhaust valve is arranged on the exhaust pipeline. Thereby, opening of the bioreactor is facilitated.

To sum up, the utility model discloses a fixed bed bioreactor's simple structure carries out the maximize utilization to the results mouth, has simplified bioreactor's tank structure, has promoted intensity.

Obviously, according to the above-mentioned contents of the present invention, many other modifications, substitutions or alterations can be made without departing from the basic technical idea of the present invention.

The above-mentioned aspects of the present invention will be described in further detail below with reference to specific embodiments in the form of examples. This should not be understood as limiting the scope of the above-described subject matter of the present invention to the following examples only. All the technologies realized based on the above contents of the present invention all belong to the scope of the present invention.

Drawings

The accompanying drawings, which form a part of the disclosure, are included to assist in understanding the disclosure, and the description provided herein and the accompanying drawings, which are related thereto, are intended to explain the disclosure, but do not constitute an undue limitation on the disclosure. In the drawings:

FIG. 1 is a schematic view of the structure of a fixed-bed bioreactor according to example 1 of the present invention.

FIG. 2 is a schematic structural view of a fixed-bed bioreactor in example 2 of the present invention.

FIG. 3 is a schematic structural view of a fixed-bed bioreactor of example 3 of the present invention.

The relevant references in the above figures are:

100-bioreactor, 110-harvest port, 120-manifold, 131-first branch, 132-second branch, 140-main valve, 150-three-way valve, 151-first valve, 152-second valve, 160-exhaust pipeline, 170-exhaust valve, 200-bottle cap, 210-sampling bottle, 220-sampling pipe, 230-first exhaust pipe, 240-first exhaust valve, 310-harvest bottle, 320-harvest pipe, 330-second exhaust pipe, 340-second exhaust valve, 400-air extractor, 510-compressed air delivery pipe, 520-air inlet valve, 530-heater.

Detailed Description

The present invention will be described more fully with reference to the accompanying drawings. Those of ordinary skill in the art will be able to implement the invention based on these descriptions. Before the present invention is described with reference to the accompanying drawings, it is to be noted that:

the technical solutions and features provided in the present invention in each part including the following description may be combined with each other without conflict.

Moreover, references to embodiments of the invention in the following description are generally only to be considered as examples of the invention, and not as all embodiments. Therefore, all other embodiments obtained by a person of ordinary skill in the art without creative efforts based on the embodiments of the present invention shall fall within the protection scope of the present invention.

With respect to the terms and units of the present invention. The terms "comprising," "having," and any variations thereof in the description and claims of this invention and the related sections are intended to cover non-exclusive inclusions.

Example 1

FIG. 1 is a schematic view of the structure of a fixed-bed bioreactor 100 according to this embodiment.

As shown in fig. 1, the fixed-bed bioreactor 100 includes:

the harvesting port 110, the harvesting port 110 is a through hole arranged on the side wall of the reactor;

a header pipe 120, wherein one end of the header pipe 120 extends into the bottom of the reactor, and the other end of the header pipe 120 extends out of the reactor after passing through the harvesting port 110;

a first branch pipe 131, one end of the first branch pipe 131 is connected with the header pipe 120, and the other end is connected with a sampling device;

a second branch 132, one end of the second branch 132 being connected to the main conduit 120, and the other end being connected to the harvesting device;

and the valve assembly comprises a three-way valve 150 arranged at the joint of the first branch pipe 131, the second branch pipe 132 and the main pipe 120 and a main valve 140 arranged on the main pipe 120, wherein the main valve 140 is a differential pressure control valve.

The sampling device comprises a sampling bottle 210, a bottle cap 200, a sampling tube 220 and a first exhaust tube 230, wherein the sampling bottle 210 is in threaded connection with the bottle cap 200, one end of the sampling tube 220 is connected with the first branch tube 131, the other end of the sampling tube passes through the bottle cap 200 and then extends into the sampling bottle 210, one end of the first exhaust tube 230 passes through the bottle cap 200 and then extends into the sampling bottle 210, the other end of the first exhaust tube is connected with an exhaust device 400, and a first exhaust valve 240 is arranged on the first exhaust tube 230.

The harvesting device comprises a harvesting bottle 310, a harvesting pipe 320 and a second air suction pipe 330, one end of the harvesting pipe 320 is connected with the second branch pipe 132, the other end of the harvesting pipe passes through the body of the harvesting bottle 310 and then extends into the harvesting bottle 310, one end of the second air suction pipe 330 passes through the body of the harvesting bottle 310 and then extends into the harvesting bottle 310, the other end of the second air suction pipe is connected with the air suction device 400, and a second air suction valve 340 is arranged on the second air suction pipe 330.

The pumping device 400 is a vacuum pump.

The reactor is provided with an exhaust pipeline 160, and the exhaust pipeline 160 is provided with an exhaust valve 170.

Example 2

FIG. 2 is a schematic view of the structure of a fixed-bed bioreactor 100 according to this embodiment.

Compared to example 1, the fixed-bed bioreactor 100 of this example has the differences: as shown in fig. 2, the valve assembly includes a first valve 151 disposed on the first branch pipe 131, a second valve 152 disposed on the second branch pipe 132, and a main valve 140 disposed on the main pipe 120.

Example 3

FIG. 3 is a schematic view of the structure of a fixed-bed bioreactor 100 according to this embodiment.

Compared to example 1, the fixed-bed bioreactor 100 of this example has the differences: as shown in fig. 3, a purging device for cleaning the first branch pipe 131 after sampling is further included.

The blowing device comprises a compressed air delivery pipe 510 and a heater 530, wherein the compressed air delivery pipe 510 is connected with the first branch pipe 131, an air inlet valve 520 is arranged on the compressed air delivery pipe 510, and the heater 530 heats the compressed air in the compressed air delivery pipe 510.

Claims

1. Fixed bed bioreactor, its characterized in that: the method comprises the following steps:

the harvesting port (110), the said harvesting port (110) is the through hole located in sidewall of the reactor;

one end of the header pipe (120) extends into the bottom of the reactor, and the other end of the header pipe (120) extends out of the reactor after penetrating through the harvesting port (110);

the first branch pipe (131), one end of the first branch pipe (131) is connected with the header pipe (120), and the other end of the first branch pipe is connected with the sampling device;

a second branch pipe (132), wherein one end of the second branch pipe (132) is connected with the header pipe (120), and the other end is connected with the harvesting device;

the valve assembly comprises a three-way valve (150) arranged at the joint of the first branch pipe (131), the second branch pipe (132) and the main pipe (120) and a main valve (140) arranged on the main pipe (120), or comprises a first valve (151) arranged on the first branch pipe (131), a second valve (152) arranged on the second branch pipe (132) and a main valve (140) arranged on the main pipe (120).

2. A fixed bed bioreactor as set forth in claim 1 wherein: the main valve (140) is a differential pressure control valve.

3. A fixed bed bioreactor as set forth in claim 2 wherein: the sampling device comprises a sampling bottle (210), a sampling tube (220) and a first air extraction tube (230), one end of the sampling tube (220) is connected with the first branch tube (131), the other end of the sampling tube penetrates through the body of the sampling bottle (210) and then extends into the sampling bottle (210), one end of the first air extraction tube (230) penetrates through the body of the sampling bottle (210) and then extends into the sampling bottle (210), the other end of the first air extraction tube is connected with an air extraction device (400), and a first air extraction valve (240) is arranged on the first air extraction tube (230).

4. A fixed bed bioreactor as set forth in claim 3 wherein: the sampling device comprises a bottle cap (200) in threaded connection with the sampling bottle (210), and the sampling tube (220) and the first suction tube (230) penetrate through the bottle cap (200) and then extend into the sampling bottle (210).

5. A fixed bed bioreactor as set forth in claim 1 wherein: and the purging device is used for cleaning the first branch pipe (131) after sampling.

6. The fixed bed bioreactor of claim 5, wherein: the purging device comprises a compressed air conveying pipe (510), the compressed air conveying pipe (510) is connected with the first branch pipe (131), and an air inlet valve (520) is arranged on the compressed air conveying pipe (510).

7. The fixed bed bioreactor of claim 6, wherein: the purging device further comprises a heater (530), and the heater (530) heats the compressed air in the compressed air delivery pipe (510).

8. A fixed bed bioreactor as set forth in claim 2 wherein: the harvesting device comprises a harvesting bottle (310), a harvesting pipe (320) and a second air suction pipe (330), one end of the harvesting pipe (320) is connected with the second branch pipe (132), the other end of the harvesting pipe penetrates through the body of the harvesting bottle (310) and then extends into the interior of the harvesting bottle (310), one end of the second air suction pipe (330) penetrates through the body of the harvesting bottle (310) and then extends into the interior of the harvesting bottle (310), the other end of the second air suction pipe is connected with an air suction device (400), and a second air suction valve (340) is arranged on the second air suction pipe (330).

9. A fixed bed bioreactor as set forth in claim 1 wherein: the reactor is provided with an exhaust pipeline (160), and the exhaust pipeline (160) is provided with an exhaust valve (170).