CN203069599U - Western blot automatic interpretation and analysis device - Google Patents
Western blot automatic interpretation and analysis device Download PDFInfo
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- CN203069599U CN203069599U CN 201320035363 CN201320035363U CN203069599U CN 203069599 U CN203069599 U CN 203069599U CN 201320035363 CN201320035363 CN 201320035363 CN 201320035363 U CN201320035363 U CN 201320035363U CN 203069599 U CN203069599 U CN 203069599U
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Abstract
The utility model discloses a western blot automatic interpretation and analysis device. The interpretation and analysis device comprises a shell, a frame, a support plate, a tray, an X-axis drive device, a Y-axis arm, a Y-axis drive device and an image acquisition device, wherein the frame is fixedly mounted in the shell, the tray is arranged on the frame, the support plate is fixedly mounted on the frame, the support plate is provided with an X-axis guide rail, the Y-axis arm is mounted on the X-axis guide rail, the X-axis drive device drives the Y-axis arm to move along the X-axis guide rail, the Y-axis arm is provided with a Y-axis guide rail, the image acquisition device is arranged on the Y-axis guide rail, the Y-axis drive device is fixedly mounted on the Y-axis arm, and the Y-axis drive device drives the image acquisition device to move along the Y-axis guide rail. The western blot automatic interpretation and analysis device can interpret all the film strips in an incubation disk in a full-automatic mode, and is free from manual operation in the whole process, so that the probability of biological contamination is completely eradicated, and the probability of infection to operators is also completely eradicated. With the western blot automatic interpretation and analysis device, the working efficiency is improved greatly.
Description
Technical field
The utility model discloses a kind of Western blot automatic identification equipment, particularly a kind of Western blot automatic interpretation analytical equipment.
Background technology
The Western blot detection technique is an advanced technology of analyzing biomacromolecule of emerging in large numbers in recent years.It is a comprehensive immunology detection technology, can adopt the SDS-PAGE technology that the protein molecule in the biological sample is separated at gel by the size of molecular weight, with the method for electrotransfer albumen be transferred to (NC, nylon or pvdf membrane) on the immobilon-p then; Perhaps adopt albumen specking three-dimensional platform with antigen coated on solid phase carrier, carry out immunology detection afterwards.Because immunology detection susceptibility height, and characteristics such as the analysis capacity is big, susceptibility is high, high specificity that Western blotting has, be to detect protein properties, express and a kind of the most frequently used method that distributes, as the quality determination of the qualitative and quantitative detection of tissue antigen, peptide molecule and viral antibody or antigen detection etc.After the Western blot testing process is finished, need carry out interpretation to testing result, the interpretation of traditional testing result is to carry out interpretation by range estimation basically, and range estimation causes interpretation difference easily owing to subjective, has the possibility of erroneous judgement.
At present, thereby the mode that realizes automatic interpretation by scanner scanning is arranged also, can stop the error of the artificial interpretation of tradition, guarantee the consistance of sentence read result.But, when by scanner Western blot being carried out interpretation, the film bar need be taken out to stick on the scanner glass platen and carry out, in this process, cause operating personnel's infection easily, there is very large bio-safety hidden danger.And, easily because of the skew of paste position, exert an influence to reading the result in the taping process.
For above-mentioned situation, at present, someone proposes a kind of Western blot automatic interpretation device, can carry out automatic interpretation to the film bar automatically, be China's invention of CN 102759617 A referring to publication number please, though this invention can realize the automatic interpretation to the film bar, but, it once can only film bar of interpretation, still needs manually to carry out the displacement of film bar after interpretation is intact, still has the not high problem of interpretation efficient.
Summary of the invention
Exist the not high problem of work efficiency at the above-mentioned Western blot interpretation device of mentioning of the prior art, the utility model provides a kind of new Western blot interpretation analytical equipment, it is shifted by the motor-driven reading device, can carry out full-automatic interpretation for all film bars of hatching in the dish automatically, not only stop the possibility of biological pollution and to operating personnel's possibility of infection, also improved work efficiency simultaneously greatly.
The technical scheme that its technical matters that solves the utility model adopts is: a kind of Western blot automatic interpretation analytical equipment, the interpretation analytical equipment comprises shell, frame, back up pad, pallet, the X-axis drive unit, the Y-axis arm, Y-axis drive unit and image collecting device, the frame fixed installation in the enclosure, pallet is arranged on the frame, back up pad is fixedly mounted on the frame, back up pad is provided with the X-axis guide rail, the Y-axis arm is installed on the X-axis guide rail, the X-axis drive unit drives the Y-axis arm along the X-axis guide rail movement, the Y-axis arm is provided with the Y-axis guide rail, image collecting device is arranged on the Y-axis guide rail, the Y-axis drive unit is fixedly mounted on the Y-axis arm, and the Y-axis drive unit drives image collecting device along the Y-axis guide rail movement.
The technical scheme that its technical matters that solves the utility model adopts further comprises:
Be installed with tray rail on the described frame, tray rail and Y-axis arm be arranged in parallel, and pallet is arranged on the tray rail, and can slide along tray rail.
Be installed with tray drive on the described frame, the motion of tray drive driving tray.
Described tray drive comprises that pallet motor and pallet be with synchronously, and pallet and pallet band synchronously are fixedly installed togather, and pallet motor-driven pallet is with motion synchronously.
Be installed with optocoupler on the described frame, be installed with catch corresponding to the optocoupler position on the pallet.
Described X-axis drive unit comprises that X-axis motor and X-axis be with synchronously, and Y-axis arm and X-axis band synchronously are fixedly installed togather, and X-axis motor-driven X-axis is with motion synchronously.
Described Y-axis drive unit comprises that y-axis motor and Y-axis be with synchronously, and image collecting device and Y-axis band synchronously are fixedly installed togather, and y-axis motor drives Y-axis and is with motion synchronously.
Described image collecting device comprises shooting headstock and camera, and camera is fixedly mounted on the shooting headstock.
Described image collecting device also comprises the LED lamp plate, and lamp plate is fixedly mounted on the shooting headstock, and lamp plate is provided with the LED lamp, and the LED lamp illuminates scope and the camera collection image range matches.
Described lamp plate below is installed with light guide plate.
The beneficial effects of the utility model are: the utility model can carry out interpretation to all film bars of hatching in the dish automatically, not only do not need in the whole process to operate manually, and can remove the error of visual observation and the trouble that sample is pasted scanning from, guaranteed the accuracy of testing result interpretation, saved the time, improve work efficiency, reduced bio-safety hidden danger.
Below in conjunction with the drawings and specific embodiments the utility model is described further.
Description of drawings
Fig. 1 is the utility model inner structure schematic perspective view.
Fig. 2 is another visual angle perspective view of the utility model inner structure.
Among the figure, 1-is hatched dish, 2-pallet, 3-frame, the 4-tray rail, the 5-pallet is with synchronously, and the 6-Y axle is with synchronously, the 7-Y axis rail, the 8-Y armshaft, 9-light guide plate, 10-lamp plate, the 11-headstock of making a video recording, the 12-camera, 13-Y spindle motor, 14-pallet motor, 15-mainboard, the 16-X axis rail, the 17-X axle is with synchronously, the 18-X spindle motor, the 20-optocoupler, 21-catch, 22-back up pad.
Embodiment
Embodiment one: present embodiment is the utility model preferred implementation, and other all its principles are identical with present embodiment or approximate with basic structure, all within the utility model protection domain.
Please in conjunction with referring to accompanying drawing 1 and accompanying drawing 2, the utility model mainly comprises shell 19 and internal mechanism, in the present embodiment, and shell 19 rear casing that only draws, face-piece does not draw.Internal mechanism is installed in the shell 19, and in the present embodiment, the internal mechanism main body is installed on the frame 3, and frame 3 is fixedly mounted in the shell 19, is installed with mainboard 15 on the frame 3, and the control circuit in the present embodiment is arranged on the mainboard 15.Secured in parallel is equipped with tray rail 4 on the frame 3, and in the present embodiment, tray rail 4 is provided with two, and two tray rails 4 be arranged in parallel, and pallet 2 is installed on the tray rail 4, can move forward and backward along tray rail 4.Be installed with tray drive mechanism on the frame 3, move forward and backward by tray drive mechanism driving tray 2, in the present embodiment, tray drive mechanism comprises that pallet motor 14 and pallet be with 5 synchronously, pallet is with 5 to be arranged in parallel with tray rail 4 synchronously, and pallet motor 14 adopts servomotors, and pallet motor 14 drives pallets and is with 5 to move synchronously, pallet 2 is with 5 to be fixedly installed togather with pallet synchronously, is with 5 pulling pallets 2 to seesaw synchronously by pallet.In the present embodiment, be installed with optocoupler 20 in frame 3, correspondence is equipped with catch 21 on the pallet 2, when catch 21 moves to optocoupler 20 places, namely triggers optocoupler 20, judges that pallet 2 moves to the inner dead point position.
In the present embodiment, be installed with back up pad 22 in frame 3 rear ends, back up pad 22 tops are installed with X-axis guide rail 16, and Y-axis arm 8 is installed on the X-axis guide rail 16, Y-axis arm 8 and the 16 vertical settings of X-axis guide rail, and can be on X-axis guide rail 16 move left and right.Be installed with the X-axis drive unit on the back up pad 22, the X-axis drive unit comprises that X-axis motor 18 and X-axis be with 17 synchronously, X-axis motor 18 is fixedly mounted on the back up pad 22, X-axis is with 17 to be arranged in parallel with X-axis guide rail 16 synchronously, X-axis motor 18 drives X-axis and is with 17 motions synchronously, Y-axis arm 8 is with 17 to be fixedly installed togather with X-axis synchronously, is with 17 can drive 8 side-to-side movements of Y-axis arm synchronously by X-axis.Y-axis guide rail 7 is installed on the Y-axis arm 8, and Y-axis guide rail 7 be arranged in parallel with U armshaft 8, and Y-axis guide rail 7 is provided with shooting headstock 11, and it is image processing circuit that shooting headstock 11 is provided with the webcam driver circuit, and shooting headstock 11 can move forward and backward along Y-axis guide rail 7.Be installed with the Y-axis drive unit on the Y-axis arm 8, the Y-axis drive unit comprises that y-axis motor 13 and Y-axis be with 6 synchronously, y-axis motor 13 is fixedly mounted on the Y-axis arm 8, y-axis motor 13 drives Y-axis and is with 6 motions synchronously, in the present embodiment, Y-axis is with 6 to be arranged in parallel with Y-axis guide rail 7 synchronously, and shooting headstock 11 is with 6 to be fixedly installed togather with Y-axis synchronously, is with 6 to drive shooting headstocks 11 and move forward and backward synchronously by Y-axis.In the present embodiment, be installed with camera 12 at shooting headstock 11, can gather the film bar image of hatching in the dish 1 by camera 12, the testing result after Western blot is handled is judged.In order to obtain clear bright image, in the present embodiment, on shooting headstock 11, also be installed with LED lamp plate 10, LED lamp plate 10 is provided with the LED illuminating lamp, can gather image-region to camera 12 by the LED illuminating lamp throws light on, lamp plate 10 bottoms are fixed with light guide plate 9, and the light that the LED illuminating lamp is sent plays the effect of sheen.
Above-described embodiment is the utility model preferred implementation, and in addition, also there are other embodiments in the utility model, and is specific as follows:
Embodiment two: the basic structure in the present embodiment is identical with embodiment's one, difference is, the mode that X-axis drive unit among the embodiment one, Y-axis drive unit and tray drive mechanism all adopt driven by servomotor to be with synchronously realizes, during concrete enforcement, also can adopt linear electric motors to realize, perhaps by realizations such as driven by motor screw mandrels.
Embodiment three: in the present embodiment, basic structure is identical with embodiment's one, and difference is that simple in structure with respect to embodiment one in the present embodiment, it does not arrange tray drive mechanism, and adopts manual mode to realize the turnover of pallet.
The utility model is divided into following a few step in use:
(1) starting switch, pallet motor 14 rotates, and is with 5 drive pallets 2 outwards to move synchronously by pallet;
(2) will hatch dish 1 is placed on the pallet 2;
(3) pallet motor 14 counter-rotatings are with 5 drive pallets 2 inwardly to move by pallet synchronously, till catch 21 is in optocoupler 20 places, judge that then it moves to inner dead point;
(4) X-axis motor 18 rotates, and is with 17 to drive 8 motions of Y-axis arm synchronously by X-axis, camera 12 is positioned at hatches above the first film bar of dish 1;
(5) y-axis motor 13 motion is with 6 to drive 11 motions of shooting headstocks by Y-axis synchronously, make on the shooting headstock 11 camera 12 along the first film bar direction to the first film bar influence gather, simultaneously the image information that collects is transferred to computing machine;
(6) X-axis motor 18 rotations are with 17 to drive the mobile lattice film bar position of Y-axis arms 8 motions by X-axis synchronously, camera 12 is positioned at hatches above the second film bar of dish 1;
Repeating step (5) and step (6) are finished all film bar image acquisition;
(7) pallet motor 14 rotates, and pallet 2 withdraws from, and will hatch dish 1 and take away, finishes the interpretation of film bar.
The utility model can automatically be finished image information collection and the sentence read result analysis of film bar, can remove the error of visual observation and the trouble that sample is pasted scanning from, have that speed is fast, discrimination is high, easy and simple to handle, characteristics that can automatic interpretation Western blot testing result.
Claims (10)
1. Western blot automatic interpretation analytical equipment, it is characterized in that: described interpretation analytical equipment comprises shell, frame, back up pad, pallet, the X-axis drive unit, the Y-axis arm, Y-axis drive unit and image collecting device, the frame fixed installation in the enclosure, pallet is arranged on the frame, back up pad is fixedly mounted on the frame, back up pad is provided with the X-axis guide rail, the Y-axis arm is installed on the X-axis guide rail, the X-axis drive unit drives the Y-axis arm along the X-axis guide rail movement, the Y-axis arm is provided with the Y-axis guide rail, image collecting device is arranged on the Y-axis guide rail, and the Y-axis drive unit is fixedly mounted on the Y-axis arm, and the Y-axis drive unit drives image collecting device along the Y-axis guide rail movement.
2. Western blot automatic interpretation analytical equipment according to claim 1, it is characterized in that: be installed with tray rail on the described frame, tray rail and Y-axis arm be arranged in parallel, and pallet is arranged on the tray rail, and can slide along tray rail.
3. Western blot automatic interpretation analytical equipment according to claim 2 is characterized in that: be installed with tray drive on the described frame, the motion of tray drive driving tray.
4. Western blot automatic interpretation analytical equipment according to claim 3 is characterized in that: described tray drive comprises that pallet motor and pallet be with synchronously, and pallet and pallet band synchronously are fixedly installed togather, and pallet motor-driven pallet is with motion synchronously.
5. Western blot automatic interpretation analytical equipment according to claim 4 is characterized in that: be installed with optocoupler on the described frame, be installed with catch corresponding to the optocoupler position on the pallet.
6. according to any described Western blot automatic interpretation analytical equipment in the claim 1 to 5, it is characterized in that: described X-axis drive unit comprises that X-axis motor and X-axis be with synchronously, Y-axis arm and X-axis band synchronously are fixedly installed togather, and X-axis motor-driven X-axis is with motion synchronously.
7. according to any described Western blot automatic interpretation analytical equipment in the claim 1 to 5, it is characterized in that: described Y-axis drive unit comprises that y-axis motor and Y-axis be with synchronously, image collecting device and Y-axis band synchronously are fixedly installed togather, and y-axis motor drives Y-axis and is with motion synchronously.
8. according to any described Western blot automatic interpretation analytical equipment in the claim 1 to 5, it is characterized in that: described image collecting device comprises shooting headstock and camera, and camera is fixedly mounted on the shooting headstock.
9. Western blot automatic interpretation analytical equipment according to claim 8, it is characterized in that: described image collecting device also comprises the LED lamp plate, the LED lamp plate is fixedly mounted on the shooting headstock, and the LED lamp plate is provided with the LED lamp, and the LED lamp illuminates scope and the camera collection image range matches.
10. Western blot automatic interpretation analytical equipment according to claim 9 is characterized in that: be installed with light guide plate below the described LED lamp plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320035363 CN203069599U (en) | 2013-01-24 | 2013-01-24 | Western blot automatic interpretation and analysis device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201320035363 CN203069599U (en) | 2013-01-24 | 2013-01-24 | Western blot automatic interpretation and analysis device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN203069599U true CN203069599U (en) | 2013-07-17 |
Family
ID=48768372
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201320035363 Expired - Lifetime CN203069599U (en) | 2013-01-24 | 2013-01-24 | Western blot automatic interpretation and analysis device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN203069599U (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103616526A (en) * | 2013-12-16 | 2014-03-05 | 深圳市亚辉龙生物科技有限公司 | Full-automatic immuoblotting analysis meter and usage method and application thereof |
| CN106781863A (en) * | 2016-12-02 | 2017-05-31 | 王昱霖 | A kind of mistake proofing formula teaching experiment platform |
-
2013
- 2013-01-24 CN CN 201320035363 patent/CN203069599U/en not_active Expired - Lifetime
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103616526A (en) * | 2013-12-16 | 2014-03-05 | 深圳市亚辉龙生物科技有限公司 | Full-automatic immuoblotting analysis meter and usage method and application thereof |
| CN103616526B (en) * | 2013-12-16 | 2015-10-28 | 深圳市亚辉龙生物科技股份有限公司 | A kind of full-automatic immunoblotting assay instrument and using method thereof and application |
| CN106781863A (en) * | 2016-12-02 | 2017-05-31 | 王昱霖 | A kind of mistake proofing formula teaching experiment platform |
| CN106781863B (en) * | 2016-12-02 | 2019-03-22 | 王昱霖 | A kind of mistake proofing formula teaching experiment platform |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee | ||
| CP03 | Change of name, title or address |
Address after: Nanshan District Nanshan street Shenzhen city Guangdong province 518000 Xing Hai Lu Lai Shan Industrial Zone 5 1-4 Patentee after: SHENZHEN YHLO BIOTECH Co.,Ltd. Address before: 5, 518054, 1-4, Lai Shan Industrial Zone, Shenzhen, Nanshan District, Guangdong Patentee before: SHENZHEN YHLO BIOTECH Co.,Ltd. |
|
| CP02 | Change in the address of a patent holder |
Address after: 518000 Shenzhen 1 Longgang biological technology plant 1, Baolong two road, Baolong street, Guangdong Patentee after: SHENZHEN YHLO BIOTECH Co.,Ltd. Address before: 518000 Guangdong, Shenzhen, Nanshan District Nanshan street, Xinghai Road, Li Shan Industrial Zone, 1-4 1-4 floors. Patentee before: SHENZHEN YHLO BIOTECH Co.,Ltd. |
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| CP02 | Change in the address of a patent holder | ||
| CX01 | Expiry of patent term |
Granted publication date: 20130717 |
|
| CX01 | Expiry of patent term |