CN202063920U - Sampling head of microbe sampling instrument - Google Patents
Sampling head of microbe sampling instrument Download PDFInfo
- Publication number
- CN202063920U CN202063920U CN 201120111470 CN201120111470U CN202063920U CN 202063920 U CN202063920 U CN 202063920U CN 201120111470 CN201120111470 CN 201120111470 CN 201120111470 U CN201120111470 U CN 201120111470U CN 202063920 U CN202063920 U CN 202063920U
- Authority
- CN
- China
- Prior art keywords
- sampling head
- sampling
- slit
- acquisition port
- acquisition
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to a sampling head of a microbe sampling instrument, which comprises a sampling head body located on the upper stream of a culture vessel. A round acquisition opening and a plurality of slots arranged at the position of the acquisition opening are arranged on the sampling head body. The plurality of slots are evenly distributed along the circumference of the acquisition opening. Each slot extends along the radial direction of the acquisition opening, and a buffer transition groove is arranged on the upper stream of each slot. Ratio between the gross area of the slots and the upper superficial area of the acquisition area meets specific conditions. The sampling head can realize constant speed sampling, the sampling is direct, and the air speed of the acquisition opening and the air speed inside a clean room are basically identical. The sampling head can accurately show concentration of the microbe inside the clean room. The sampling head design optimizes impact velocity, ensures biology efficiency, and improves acquisition efficiency of active particles conforming to the international standardization organization (ISO) 14698-1 standard. Furthermore, the sampling head can easily identify whether the microbe is effective or not.
Description
Technical field
The utility model relates to a kind of microorganism sampler, particularly its sampling head.
Background technology
Along with the further investigation of biotechnology and the continuous expansion of its Application Areas, Biosafety is more and more paid much attention to.Because the manipulation object of biotechnology all is the organism of microorganism, viable cell and so on, or their recombinant chou, varient, exist and may cause communicate illness, harm operator ' s health so that influence the negative factor of environment.Particularly in engineered experimental study, exist the potential hazard that is difficult to predict.In addition, also very strict at pharmaceutical industry to the control requirement of microorganism, therefore.The detection of microorganism is very important.
The acquisition mode of microorganism sampler of the prior art mainly contains two kinds, a kind of employing slit collecting method, the selected corresponding time of the sampled air amount that is determined is called one-period, each cycle culture dish can both one week of Rotating with Uniform (360 °), the bacterium particle that is sampled is evenly distributed on by slit on the annulus of plate culture dish, obtains the DYNAMIC DISTRIBUTION of environmental bacteria in the sampling process according to the distribution of bacterium colony in it; Another kind is the pin hole method, and during sampling, band puffball air passes through micropore at a high speed, evenly impinged upon the agar surface in the culture dish, it is all even fully that these living microorganisms obtain nutrition at agar surface, in culturing process, dynamic rehydration process takes place fast, high-speed rapid growth, thus obtain a result.Yet this dual mode all can not be distinguished the miscount problem that has or not artificial disturbing influence to cause, and can not guarantee can not destroy when carrying out microbe sampling the laminar flow in clean working spaces and the pharmaceutical equipment.
Summary of the invention
Technical problem to be solved in the utility model is to overcome the deficiencies in the prior art, a kind of improved microorganism sampler is provided, it can catch microorganism expeditiously, effectively distinguish the miscount problem that artificial interference causes simultaneously, realize the isokinetic sampling, effectively avoid destruction the clean environment inner laminar flow.
For solving the problems of the technologies described above, the utility model adopts following technical scheme:
A kind of sampling head of microorganism sampler, comprise the sampling head body that is positioned at the culture dish upstream, the sampling head body has circular acquisition port and is positioned at a plurality of slits at acquisition port place, described a plurality of slit is along the circumferential uniform distribution of acquisition port, each described slit is along the radially extension of acquisition port, and be provided with the buffering ramp pan in the upstream of each slit, the design of described sampling head meets the following conditions:
S Slit=b*l*t
Wherein: S
Slit
The total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number; S
Slit
The top surface area of expression acquisition port; V
Bump
The expression microorganism strikes the speed that culture dish needs; V
Sampling
Expression sampling flow velocity.
Preferably, the material of described sampling head body (1) is the 316L stainless steel, can be fit to the variety of way sterilization.Described buffering ramp pan is tubaeform that the direction towards slit narrows down gradually from the upper surface of acquisition port.
Because adopt the enforcement of above technical scheme, the utility model compared with prior art has following advantage:
Sampling head of the present utility model can be realized the isokinetic sampling, and sampling is direct, and the wind speed of acquisition port and clean indoor air velocity keep basically identical, can accurately reflect the concentration of microorganism in the clean room.During sampling, band puffball air is evenly impinged upon the agar surface in the culture dish at a high speed by a plurality of slits, the sampling head design optimization stroke speed, guaranteed biology efficient.Improved the collecting efficiency of the active particle that meets the ISO14698-1 standard; Whether in addition, using the utility model can be easy to pick out is effective microbe, and only the microorganism at the slit location place is effectively, and all the other are local, and then explanation does not collect in gatherer process if there is microorganism to exist, for artificial the interference causes.
Description of drawings
Below in conjunction with specific embodiment, the utility model is described in more detail.
Fig. 1 is the perspective exploded view that includes the microorganism sampler of the utility model sampling head;
Fig. 2 is the partial schematic diagram that includes the microorganism sampler of the utility model sampling head;
Fig. 3 is the main cross-sectional schematic according to sampling head of the present utility model;
Fig. 4 is an A place enlarged view among Fig. 3;
Wherein: 1, sampling head body; 10, acquisition port; 11, slit; 12, buffering ramp pan; 2, culture dish; 3, culture dish support; 4, base; B, slit width.
Embodiment
As shown in Figure 1, microorganism sampler comprises sampling head, culture dish 2, is used to support the culture dish support 3 and the base 4 of culture dish 2, and the bottom of base 4 is connected with vacuum source or blower fan.The utility model relates to the improvement to wherein sampling head.
Referring to Fig. 1 to Fig. 4, the sampling head body 1 that comprises the 316L stainless steel according to the sampling head of present embodiment, sampling head body 1 has acquisition port 10 and is positioned at a plurality of slits 11 of the up/down perforation on the sampling head body 1 at acquisition port 10 places, promptly is culture dish 2 under the slit 11.As seen from the figure, a plurality of slits 11 are along the circumferential uniform distribution of acquisition port 10, and each described slit 11 is along the radially extension of acquisition port 10, and are provided with buffering ramp pan 12 in the upstream of each slit 11, and the design of described sampling head meets the following conditions:
Wherein: S Slit The total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number;S
Always The top surface area of expression acquisition port; V Bump The expression microorganism strikes the speed that culture dish needs; V Sampling Expression sampling flow velocity, V Sampling Numerical value equate with the clean environment laminar velocity.
Satisfy the sampling head of above condition and can realize the isokinetic sampling.During sampling, described base 4 bottoms are connected with vacuum source or blower fan, and guarantee certain flow; Sampling head 1 top forms the top-down air-flow with clean environment laminar flow constant speed, and after air communication was crossed slit 11, flow velocity increased to V
Bump,
Thereby microorganism is effectively impinged upon on the substratum in the culture dish 3.
According to sampling head design optimization of the present utility model stroke speed, guaranteed biology efficient.Improved the collecting efficiency of the active particle that meets the ISO14698-1 standard; Whether in addition, using the utility model can be easy to pick out is effective microbe, and only the microorganism at the slit location place is effectively, and all the other are local, and then explanation does not collect in gatherer process if there is microorganism to exist, for artificial the interference causes.
More than the utility model has been done detailed description; its purpose is to allow the personage that is familiar with this art can understand content of the present utility model and is implemented; can not limit protection domain of the present utility model with this; all equivalences of doing according to spirit of the present utility model change or modify, and all should be encompassed in the protection domain of the present utility model
Claims (3)
1. the sampling head of a microorganism sampler, comprise the sampling head body (1) that is positioned at culture dish (2) upstream, described sampling head body (1) has circular acquisition port (10) and is positioned at a plurality of slits (11) that described acquisition port (10) is located, it is characterized in that: described a plurality of slits (11) are along the circumferential uniform distribution of described acquisition port (10), each described slit (11) is along the radially extension of described acquisition port (10), and be provided with buffering ramp pan (12) in the upstream of described each slit (11), the design of described sampling head meets the following conditions:
S Slit=b*l*t is wherein:
S SlitThe total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number;
S AlwaysThe top surface area of expression acquisition port;
V BumpThe expression microorganism strikes the speed that culture dish needs;
V SamplingExpression sampling flow velocity.
2. the sampling head of microorganism sampler according to claim 1, it is characterized in that: the material of described sampling head body (1) is the 316L stainless steel.
3. the sampling head of microorganism sampler according to claim 1 and 2 is characterized in that: described buffering ramp pan (12) is tubaeform that the direction towards described slit (11) narrows down gradually from the upper surface of described acquisition port (10).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120111470 CN202063920U (en) | 2011-04-15 | 2011-04-15 | Sampling head of microbe sampling instrument |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120111470 CN202063920U (en) | 2011-04-15 | 2011-04-15 | Sampling head of microbe sampling instrument |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202063920U true CN202063920U (en) | 2011-12-07 |
Family
ID=45057789
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120111470 Expired - Lifetime CN202063920U (en) | 2011-04-15 | 2011-04-15 | Sampling head of microbe sampling instrument |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202063920U (en) |
-
2011
- 2011-04-15 CN CN 201120111470 patent/CN202063920U/en not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: JIANGSU SUJING GROUP CO. LTD. Free format text: FORMER OWNER: SUZHOU SUJING INSTRUMENT AUTOMATIC CONTROL EQUIPMENT CO., LTD. Effective date: 20121217 Owner name: SUZHOU SUJING INSTRUMENT AUTOMATIC CONTROL EQUIPME Effective date: 20121217 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20121217 Address after: 215122 Suzhou Industrial Park, Jiangsu, the only new road, No. 2 Patentee after: Jiangsu Sujing Group Co., Ltd. Patentee after: Suzhou Sujing Group Automation Instrument Equipment Corporation Address before: 215122 Suzhou Industrial Park, Suzhou Province, the only new road, No. 2, Jiangsu Patentee before: Suzhou Sujing Group Automation Instrument Equipment Corporation |
|
| AV01 | Patent right actively abandoned |
Granted publication date: 20111207 Effective date of abandoning: 20130731 |
|
| RGAV | Abandon patent right to avoid regrant |