CN102220235B - Sampling head of microbial sampler - Google Patents
Sampling head of microbial sampler Download PDFInfo
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- CN102220235B CN102220235B CN 201110095010 CN201110095010A CN102220235B CN 102220235 B CN102220235 B CN 102220235B CN 201110095010 CN201110095010 CN 201110095010 CN 201110095010 A CN201110095010 A CN 201110095010A CN 102220235 B CN102220235 B CN 102220235B
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- sampling head
- acquisition port
- slit
- sampling
- slits
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
Abstract
The invention relates to a sampling head of a microbial sampler. The sampling head comprises a sampling head body which is positioned on an upstream of a culture dish, the sampling head body has a circular acquisition port and a plurality of slits on the acquisition port, the slits are uniformly distributed along a circumference of the acquisition port, each slit extends along a radial direction of the acquisition port, and the upstream of each slit is provided with a buffer groove, and a ratio of a total area of all the slits to an upper surface area of the acquisition port can satisfy a specific condition. The sampling head of the present invention allows isokinetic sampling to be realized, sampling to be direct, a wind velocity of the acquisition port to be basically consistent with the wind velocity of an inner clean room, a concentration of a microbe to be accurately reflected, an impact velocity to be optimized through the design of the sampling head, the biological efficiency to be guaranteed, the acquisition efficiency of active particles which accords with the ISO14698-1 standard to be improved, and a case that whether the microbe is effective or not to be easily discriminated.
Description
Technical field
The present invention relates to a kind of microorganism sampler, particularly its sampling head.
Background technology
Along with the further investigation of biotechnology and the continuous expansion of its Application Areas, Biosafety is more and more paid much attention to.Because the manipulation object of biotechnology all is the organism of microorganism, viable cell and so on, or their recombinant chou, varient, exist and may cause communicate illness, harm operator ' s health so that influence the negative factor of environment.Particularly in engineered experimental study, exist the potential hazard that is difficult to predict.In addition, also very strict at pharmaceutical industry to the control requirement of microorganism, therefore.The detection of microorganism is very important.
The acquisition mode of microorganism sampler of the prior art mainly contains two kinds, a kind of employing slit collecting method, the selected corresponding time of the sampled air amount that is determined is called one-period, each cycle culture dish can both one week of Rotating with Uniform (360 °), the bacterium particle that is sampled is evenly distributed on by slit on the annulus of plate culture dish, obtains the DYNAMIC DISTRIBUTION of environmental bacteria in the sampling process according to the distribution of bacterium colony in it; Another kind is the pin hole method, and during sampling, band puffball air passes through micropore at a high speed, evenly impinged upon the agar surface in the culture dish, it is all even fully that these living microorganisms obtain nutrition at agar surface, in culturing process, dynamic rehydration process takes place fast, high-speed rapid growth, thus obtain a result.Yet this dual mode all can not be distinguished the miscount problem that has or not artificial disturbing influence to cause, and can not guarantee can not destroy when carrying out microbe sampling the laminar flow in clean working spaces and the pharmaceutical equipment.
Summary of the invention
Technical problem to be solved by this invention is to overcome the deficiencies in the prior art, a kind of improved microorganism sampler is provided, and it can catch microorganism expeditiously, effectively distinguishes the miscount problem that artificial interference causes simultaneously, realize the isokinetic sampling, effectively avoid destruction the clean environment inner laminar flow.
For solving the problems of the technologies described above, the present invention adopts following technical scheme:
A kind of sampling head of microorganism sampler, comprise the sampling head body that is positioned at the culture dish upstream, the sampling head body has circular acquisition port and is positioned at a plurality of slits at acquisition port place, described a plurality of slit is along the circumferential uniform distribution of acquisition port, each described slit is along the radially extension of acquisition port, and be provided with the buffering ramp pan in the upstream of each slit, the design of described sampling head meets the following conditions:
Wherein:S
Slit The total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number;S
Always The top surface area of expression acquisition port;V
Bump The expression microorganism strikes the speed that culture dish needs;V
Sampling Expression sampling flow velocity.
Preferably, the material of described sampling head body (1) is the 316L stainless steel, can be fit to the variety of way sterilization.Described buffering ramp pan is tubaeform that the direction towards slit narrows down gradually from the upper surface of acquisition port.
Because adopt the enforcement of above technical scheme, the present invention compared with prior art has following advantage:
Sampling head of the present invention can be realized the isokinetic sampling, and sampling is direct, and the wind speed of acquisition port and clean indoor air velocity keep basically identical, can accurately reflect the concentration of microorganism in the clean room.During sampling, band puffball air is evenly impinged upon the agar surface in the culture dish at a high speed by a plurality of slits, the sampling head design optimization stroke speed, guaranteed biology efficient.Improved the collecting efficiency of the active particle that meets the ISO14698-1 standard; Whether in addition, using the present invention can be easy to pick out is effective microbe, and only the microorganism at the slit location place is effectively, and all the other are local, and then explanation does not collect in gatherer process if there is microorganism to exist, for artificial the interference causes.
Description of drawings
Below in conjunction with specific embodiment, the present invention will be further described in detail.
Fig. 1 is the perspective exploded view that includes the microorganism sampler of sampling head of the present invention;
Fig. 2 is the partial schematic diagram that includes the microorganism sampler of sampling head of the present invention;
Fig. 3 is the main cross-sectional schematic according to sampling head of the present invention;
Fig. 4 is an A place enlarged view among Fig. 3;
Wherein: 1, sampling head body; 10, acquisition port; 11, slit; 12, buffering ramp pan; 2, culture dish; 3, culture dish support; 4, base; B, slit width.
Embodiment
As shown in Figure 1, microorganism sampler comprises sampling head, culture dish 2, is used to support the culture dish support 3 and the base 4 of culture dish 2, and the bottom of base 4 is connected with vacuum source or blower fan.The present invention relates to improvement to wherein sampling head.
Referring to Fig. 1 to Fig. 4, the sampling head body 1 that comprises the 316L stainless steel according to the sampling head of present embodiment, sampling head body 1 has acquisition port 10 and is positioned at a plurality of slits 11 of the up/down perforation on the sampling head body 1 at acquisition port 10 places, promptly is culture dish 2 under the slit 11.As seen from the figure, a plurality of slits 11 are along the circumferential uniform distribution of acquisition port 10, and each described slit 11 is along the radially extension of acquisition port 10, and are provided with buffering ramp pan 12 in the upstream of each slit 11, and the design of described sampling head meets the following conditions:
Wherein:S
Slit The total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number;S
Always The top surface area of expression acquisition port;V
Bump The expression microorganism strikes the speed that culture dish needs;V
Sampling Expression sampling flow velocity,V
Sampling Numerical value equate with the clean environment laminar velocity.
Satisfy the sampling head of above condition and can realize the isokinetic sampling.During sampling, described base 4 bottoms are connected with vacuum source or blower fan, and guarantee certain flow; Sampling head 1 top forms the top-down air-flow with clean environment laminar flow constant speed, and after air communication was crossed slit 11, flow velocity increased toV
Bump, Thereby microorganism is effectively impinged upon on the substratum in the culture dish 3.
According to sampling head design optimization of the present invention stroke speed, guaranteed biology efficient.Improved the collecting efficiency of the active particle that meets the ISO14698-1 standard; Whether in addition, using the present invention can be easy to pick out is effective microbe, and only the microorganism at the slit location place is effectively, and all the other are local, and then explanation does not collect in gatherer process if there is microorganism to exist, for artificial the interference causes.
More than the present invention has been done detailed description; its purpose is to allow the personage that is familiar with this art can understand content of the present invention and is implemented; can not limit protection scope of the present invention with this; all equivalences of doing according to spirit of the present invention change or modify, and all should be encompassed in protection scope of the present invention
Claims (2)
1. the sampling head of a microorganism sampler, comprise the sampling head body (1) that is positioned at culture dish (2) upstream, described sampling head body (1) has circular acquisition port (10) and is positioned at a plurality of slits (11) that described acquisition port (10) is located, it is characterized in that: described a plurality of slits (11) are along the circumferential uniform distribution of described acquisition port (10), each described slit (11) is along the radially extension of described acquisition port (10), and be provided with buffering ramp pan (12) in the upstream of described each slit (11), described buffering ramp pan (12) is tubaeform that the direction towards described slit (11) narrows down gradually from the upper surface of described acquisition port (10), and the design of described sampling head meets the following conditions:
S
Slit=b*l*t
Wherein: S
SlitThe total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number; S
AlwaysThe top surface area of expression acquisition port; V
BumpThe expression microorganism strikes the speed that culture dish needs; V
SamplingExpression sampling flow velocity.
2. the sampling head of microorganism sampler according to claim 1, it is characterized in that: the material of described sampling head body (1) is the 316L stainless steel.
Priority Applications (1)
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| CN 201110095010 CN102220235B (en) | 2011-04-15 | 2011-04-15 | Sampling head of microbial sampler |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 201110095010 CN102220235B (en) | 2011-04-15 | 2011-04-15 | Sampling head of microbial sampler |
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| CN102220235A CN102220235A (en) | 2011-10-19 |
| CN102220235B true CN102220235B (en) | 2013-07-31 |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| ITRM20130128U1 (en) | 2013-07-23 | 2015-01-24 | Particle Measuring Systems S R L | DEVICE FOR MICROBIAL AIR SAMPLING |
| CN103773678B (en) * | 2014-01-27 | 2015-02-04 | 浙江大学 | Water supply pipeline biological membrane sampler and method thereof |
| KR20210091183A (en) | 2018-11-16 | 2021-07-21 | 파티클 머슈어링 시스템즈, 인크. | Particle Sampling Systems and Methods for Robot Controlled Manufacturing Barrier Systems |
| JP2023509830A (en) | 2020-01-21 | 2023-03-10 | パーティクル・メージャーリング・システムズ・インコーポレーテッド | Robot control for aseptic processing |
Citations (5)
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|---|---|---|---|---|
| DE19503663A1 (en) * | 1994-01-28 | 1995-08-03 | Joerg Prof Dr Hartung | Sampling of micro organisms in the ambient air |
| CN101659921A (en) * | 2009-09-02 | 2010-03-03 | 青岛众瑞智能仪器有限公司 | Full automatic microorganism sampler |
| CN101875901A (en) * | 2009-04-29 | 2010-11-03 | 武汉爱维安防科技有限公司 | Multi-slit type air microorganism sampler |
| CN101974418A (en) * | 2010-10-24 | 2011-02-16 | 青岛众瑞智能仪器有限公司 | Miniature personal microbial air sampler |
| CN101974419A (en) * | 2010-10-24 | 2011-02-16 | 青岛众瑞智能仪器有限公司 | Pathogenic microorganism aerosol pollution colony monitoring system |
-
2011
- 2011-04-15 CN CN 201110095010 patent/CN102220235B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE19503663A1 (en) * | 1994-01-28 | 1995-08-03 | Joerg Prof Dr Hartung | Sampling of micro organisms in the ambient air |
| CN101875901A (en) * | 2009-04-29 | 2010-11-03 | 武汉爱维安防科技有限公司 | Multi-slit type air microorganism sampler |
| CN101659921A (en) * | 2009-09-02 | 2010-03-03 | 青岛众瑞智能仪器有限公司 | Full automatic microorganism sampler |
| CN101974418A (en) * | 2010-10-24 | 2011-02-16 | 青岛众瑞智能仪器有限公司 | Miniature personal microbial air sampler |
| CN101974419A (en) * | 2010-10-24 | 2011-02-16 | 青岛众瑞智能仪器有限公司 | Pathogenic microorganism aerosol pollution colony monitoring system |
Non-Patent Citations (4)
| Title |
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| 曲建翘.空气微生物采样器.《仪器仪表与分析监测》.1991,(第02期), |
| 空气微生物采样器;曲建翘;《仪器仪表与分析监测》;19911231(第02期);58-59,45 * |
| 空气微生物采样器的进展;陈烈贤等;《卫生研究》;19941231;第23卷;138-144 * |
| 陈烈贤等.空气微生物采样器的进展.《卫生研究》.1994,第23卷 |
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| CN102220235A (en) | 2011-10-19 |
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