CN202030766U - Microorganism sampler - Google Patents

Microorganism sampler Download PDF

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Publication number
CN202030766U
CN202030766U CN 201120111498 CN201120111498U CN202030766U CN 202030766 U CN202030766 U CN 202030766U CN 201120111498 CN201120111498 CN 201120111498 CN 201120111498 U CN201120111498 U CN 201120111498U CN 202030766 U CN202030766 U CN 202030766U
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sampling
slit
controller
culture dish
microorganism sampler
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CN 201120111498
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金惠琴
陈建
刘嘉
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Jiangsu Sujing Group Co Ltd
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SUZHOU SUJING GROUP AUTOMATION INSTRUMENT EQUIPMENT Corp
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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00Constructional details, e.g. recesses, hinges
    • C12M23/02Form or structure of the vessel
    • C12M23/10Petri dish

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The utility model relates to a microorganism sampler. The microorganism sampler comprises a collecting unit and a control unit, wherein the collecting unit includes a collecting head with a collecting port and slits, a culture dish arranged below the collecting port, a culture dish bracket for supporting the culture dish, as well as a base; the bottom of the base is connected with a blower through an air pipe; the control unit includes a controller, a display screen, and control keys arranged on a controller panel for controlling sampling; the controller and the sampling head are arranged in a split manner; and the control unit further includes a remote controller separated from the controller and used for long-distance sampling control. The controller and the sampling head of the microorganism sampler are arranged in a split manner, and the remote controller can be used for long-distance remote-controlled sampling, so that operators are separated from the detection environment, which can prevent the operators from interfering with the detection environment and prevent the hazardous environment from affecting the personal health.

Description

A kind of microorganism sampler
Technical field
The utility model relates to a kind of microorganism sampler.
Background technology
Along with the further investigation of biotechnology and the continuous expansion of its Application Areas, Biosafety is more and more paid much attention to.Because the manipulation object of biotechnology all is the organism of microorganism, viable cell and so on, or their recombinant chou, varient, exist and may cause communicate illness, harm operator ' s health so that influence the negative factor of environment.Particularly in engineered experimental study, exist the potential hazard that is difficult to predict.In addition, also very strict at pharmaceutical industry to the control requirement of microorganism, therefore.The detection of microorganism is very important.And at present, the microorganism sampler that is used to gather microorganism substantially all is a desk-top instrument, operating main body is that operation interface and collection head connect together, and now in the testing environment a lot of places do not allow personnel and disturb and cause that environment variation or hostile environment influence HUMAN HEALTH, present microorganism sampler can not satisfy the requirement of microorganism detection.
In addition, the acquisition mode at microorganism sampler of prior art mainly contains two kinds, a kind of employing slit collecting method, the selected corresponding time of the sampled air amount that is determined is called one-period, each cycle culture dish can both one week of Rotating with Uniform (360 °), the bacterium particle that is sampled is evenly distributed on by slit on the annulus of plate culture dish, obtains the DYNAMIC DISTRIBUTION of environmental bacteria in the sampling process according to the distribution of bacterium colony in it; Another kind is the pin hole method, and during sampling, band puffball air passes through micropore at a high speed, evenly impinged upon the agar surface in the culture dish, it is all even fully that these living microorganisms obtain nutrition at agar surface, in culturing process, dynamic rehydration process takes place fast, high-speed rapid growth, thus obtain a result.Yet this dual mode all can not be distinguished the miscount problem that has or not artificial disturbing influence to cause, and can not guarantee can not destroy when carrying out microbe sampling the laminar flow in clean working spaces and the pharmaceutical equipment.
Summary of the invention
Technical problem to be solved in the utility model is to overcome the deficiencies in the prior art, a kind of improved microorganism sampler is provided, realize that operator separate with testing environment, thus avoid operator to the interference of testing environment and hostile environment to health effects.
For solving above technical problem, the utility model is taked following technical scheme: a kind of microorganism sampler, comprise collecting unit and control unit, wherein said collecting unit comprises the sampling head with acquisition port and slit, be arranged on the culture dish of acquisition port below, be used to support the culture dish support and the base of culture dish, the bottom of base is connected with blower fan by tracheae, described control unit comprises controller, display screen, be arranged on the operating key that can be used in the control sampling on the controller panel, wherein: controller and sampling head split are provided with, described control unit also comprises the telepilot that separates setting with controller, and described telepilot is used at a distance sampling being controlled.
The setting of described telepilot can be implemented with reference to existing telecontrol engineering.
According to further embodiment of the present utility model: described slit has a plurality of, and a plurality of slit is along the circumferential uniform distribution of described acquisition port.Preferably, described acquisition port is circular, and each described slit is along the radially extension of acquisition port.Upstream at described each slit also is provided with the buffering ramp pan, and the design of described sampling head meets the following conditions:
Wherein:S Slit The total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number;S Always The top surface area of expression acquisition port;V Bump The expression microorganism strikes the speed that culture dish needs;V Sampling Expression sampling flow velocity.
Above-mentioned buffering ramp pan is tubaeform that the direction towards described slit narrows down gradually from the upper surface of acquisition port.
Take above-mentioned sampling head design, can realize the isokinetic sampling, sampling is direct, and the wind speed of acquisition port and clean indoor air velocity keep basically identical, can accurately reflect the concentration of microorganism in the clean room.During sampling, band puffball air is evenly impinged upon the agar surface in the culture dish at a high speed by a plurality of slits, the sampling head design optimization stroke speed, guaranteed biology efficient.Improved the collecting efficiency of the active particle that meets the ISO14698-1 standard; Whether in addition, taking this sampling head can also be easy to pick out is effective microbe, and only the microorganism at the slit location place is effectively, and all the other are local, and then explanation does not collect in gatherer process if there is microorganism to exist, for artificial the interference causes.
According to the utility model, the material of sampling head, base and controller all is preferably the 316L stainless steel, meets clean environment and uses standard.Preferably, described tracheae is the expansion pipe of vary in length, and so, tracheae can extend or shorten according to the actual measurement environment.
Owing to adopt the enforcement of above technical scheme, the utility model compared with prior art has following advantage: the controller of microorganism sampler of the present utility model and sampling head split are provided with, also can utilize telepilot to carry out the long-distance remote control sampling, can realize separating of operator and testing environment, avoid operator to the interference of testing environment and hostile environment to health effects.
Description of drawings
Below in conjunction with specific embodiment, the utility model is described in more detail.
Fig. 1 is the structural representation according to microorganism sampler of the present utility model;
Fig. 2 is the partial exploded view according to the collecting unit of microorganism sampler of the present utility model;
Fig. 3 is the partial schematic diagram according to the sampling head of microorganism sampler of the present utility model;
Fig. 4 is the main cross-sectional schematic according to sampling head of the present utility model;
Fig. 5 is an A place enlarged view among Fig. 3;
Wherein: 1, sampling head; 10, acquisition port; 11, slit; 12, buffering ramp pan; 2, culture dish; 3, culture dish support; 4, base; B, slit width; 5, tracheae; 6, blower fan; 7, controller; 8, display screen; 9, operating key; 71, telepilot.
Embodiment
Shown in Fig. 1 to 5, microorganism sampler comprises sampling unit and control unit, and wherein sampling unit comprises sampling head 1, culture dish 2, is used to support the culture dish support 3 and the base 4 of culture dish 2, and the bottom of base 4 is connected with blower fan 6 by tracheae 5.Control unit comprises controller 7, display screen 8, be arranged on the operating key 9 that can be used in the control sampling on controller 7 panels, be used for the telepilot 71 at a distance sampling controlled, so, except that using operating key 9 control samplings, the 71 pairs of samplings that also can use a teleswitch are at a distance controlled.Because controller 7 and sampling head 1 split setting, therefore, can realize separating of operator and testing environment, avoid operator to the interference of testing environment and hostile environment to health effects.
Base 2 can be fixed according to environment for use, but also the special-purpose trivet of adapted uses flexibly; Tracheae 4 can extend or shorten according to the actual measurement environment.
The utility model also relates to the improvement to sampling head 1, and is specific as follows:
Sampling head 1 has a plurality of slits 11, and a plurality of slits 11 are along the circumferential uniform distribution of acquisition port 10, each described slit 11 is along the radially extension of acquisition port 10, and is provided with buffering ramp pan 12 in the upstream of each slit 11, and the design of described sampling head meets the following conditions:
Figure 834999DEST_PATH_IMAGE002
Wherein:S Slit The total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number;S Always The top surface area of expression acquisition port;V Bump The expression microorganism strikes the speed that culture dish needs;V Sampling Expression sampling flow velocity,V Sampling Numerical value equate with the clean environment laminar velocity.
Satisfy the sampling head of above condition and can realize the isokinetic sampling.During sampling, sampling head 1 top forms the top-down air-flow with clean environment laminar flow constant speed, and after air communication was crossed slit 11, flow velocity increased toV Bump, Thereby microorganism is effectively impinged upon on the substratum in the culture dish 2.
According to sampling head design optimization of the present utility model stroke speed, guaranteed biology efficient.Improved the collecting efficiency of the active particle that meets the ISO14698-1 standard; Whether in addition, using the utility model can be easy to pick out is effective microbe, and only the microorganism at the slit location place is effectively, and all the other are local, and then explanation does not collect in gatherer process if there is microorganism to exist, for artificial the interference causes.
To sum up, microorganism sampler of the present utility model, its sampling head adopts the constant speed principle design, can not break the laminar flow of ring clean environment; Use split-type design, can avoid personnel operation that sampling is caused human intervention.
More than the utility model has been done detailed description; its purpose is to allow the personage that is familiar with this art can understand content of the present utility model and is implemented; can not limit protection domain of the present utility model with this; all equivalences of doing according to spirit of the present utility model change or modify, and all should be encompassed in the protection domain of the present utility model

Claims (7)

1. microorganism sampler, comprise collecting unit and control unit, wherein said collecting unit comprises the sampling head (1) of have acquisition port (10) and slit (11), be arranged at the culture dish (2) of described acquisition port (10) below, be used to support the culture dish support (3) and the base (4) of culture dish (2), the bottom of base (4) is connected with blower fan (6) by tracheae (5), described control unit comprises controller (7), display screen (8), be arranged on the operating key (9) that can be used in the control sampling on controller (7) panel, it is characterized in that: described controller (7) and described sampling head (1) split setting, described control unit also comprises the telepilot (71) that separates setting with described controller (7), and described telepilot (71) is used at a distance sampling being controlled.
2. microorganism sampler according to claim 1 is characterized in that: described slit (11) has a plurality of, and a plurality of slit (11) is along the circumferential uniform distribution of described acquisition port (10).
3. microorganism sampler according to claim 2 is characterized in that: described acquisition port (10) is for circular, and each described slit (11) is along the radially extension of described acquisition port (10).
4. microorganism sampler according to claim 2 is characterized in that: be provided with buffering ramp pan (12) in the upstream of described each slit (11), the design of described sampling head meets the following conditions:
Wherein: S SlitThe total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number; S AlwaysThe top surface area of expression acquisition port; V BumpThe expression microorganism strikes the speed that culture dish needs; V SamplingExpression sampling flow velocity.
5. microorganism sampler according to claim 4 is characterized in that: described buffering ramp pan (12) is tubaeform that the direction towards described slit (11) narrows down gradually from the upper surface of described acquisition port (10).
6. microorganism sampler according to claim 1 is characterized in that: the material of described sampling head (1), base (4) and described controller (7) is the 316L stainless steel.
7. microorganism sampler according to claim 1 is characterized in that: described tracheae (5) is the expansion pipe of vary in length.
CN 201120111498 2011-04-15 2011-04-15 Microorganism sampler Expired - Lifetime CN202030766U (en)

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Cited By (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103575836A (en) * 2012-07-25 2014-02-12 北京市北分仪器技术有限责任公司 Sample introduction system
CN105547941A (en) * 2015-12-14 2016-05-04 江苏苏净集团有限公司 Raising dust detecting device
CN106479875A (en) * 2016-10-09 2017-03-08 广东环凯微生物科技有限公司 A kind of adjustable flow sieve aperture impacting type compressed air microorganism sampler
CN106482988A (en) * 2016-12-13 2017-03-08 北京大学 A kind of Portable high-flow air sampler
CN106995779A (en) * 2017-05-03 2017-08-01 浙江天科高新技术发展有限公司 It is a kind of to be used for inner-walls of duct and the microorganism sampler and its method for sampling on crack surface
CN112725164A (en) * 2020-12-14 2021-04-30 北京市理化分析测试中心 Solid surface microorganism sampler
CN113005027A (en) * 2020-11-25 2021-06-22 中国矿业大学 Device and method for measuring distribution of microbial particles in air

Cited By (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103575836A (en) * 2012-07-25 2014-02-12 北京市北分仪器技术有限责任公司 Sample introduction system
CN105547941A (en) * 2015-12-14 2016-05-04 江苏苏净集团有限公司 Raising dust detecting device
CN105547941B (en) * 2015-12-14 2019-04-05 江苏苏净集团有限公司 A kind of fugitive dust detection device
CN106479875A (en) * 2016-10-09 2017-03-08 广东环凯微生物科技有限公司 A kind of adjustable flow sieve aperture impacting type compressed air microorganism sampler
CN106482988A (en) * 2016-12-13 2017-03-08 北京大学 A kind of Portable high-flow air sampler
CN106995779A (en) * 2017-05-03 2017-08-01 浙江天科高新技术发展有限公司 It is a kind of to be used for inner-walls of duct and the microorganism sampler and its method for sampling on crack surface
CN106995779B (en) * 2017-05-03 2023-06-02 浙江天科高新技术发展有限公司 Microorganism sampler for inner wall and crack surface of pipeline and sampling method thereof
CN113005027A (en) * 2020-11-25 2021-06-22 中国矿业大学 Device and method for measuring distribution of microbial particles in air
CN112725164A (en) * 2020-12-14 2021-04-30 北京市理化分析测试中心 Solid surface microorganism sampler
CN112725164B (en) * 2020-12-14 2023-02-17 北京市理化分析测试中心 Solid surface microorganism sampler

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Owner name: SUZHOU SUJING INSTRUMENT AUTOMATIC CONTROL EQUIPME

Effective date: 20121231

Owner name: JIANGSU SUJING GROUP CO. LTD.

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Address after: 215122 Suzhou Industrial Park, Jiangsu, the only new road, No. 2

Patentee after: Jiangsu Sujing Group Co., Ltd.

Patentee after: Suzhou Sujing Group Automation Instrument Equipment Corporation

Address before: 215122 Suzhou Industrial Park, Suzhou Province, the only new road, No. 2, Jiangsu

Patentee before: Suzhou Sujing Group Automation Instrument Equipment Corporation

CX01 Expiry of patent term

Granted publication date: 20111109

CX01 Expiry of patent term