CN202063918U - On-line multi-point type microorganism collecting system - Google Patents
On-line multi-point type microorganism collecting system Download PDFInfo
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- CN202063918U CN202063918U CN 201120111495 CN201120111495U CN202063918U CN 202063918 U CN202063918 U CN 202063918U CN 201120111495 CN201120111495 CN 201120111495 CN 201120111495 U CN201120111495 U CN 201120111495U CN 202063918 U CN202063918 U CN 202063918U
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- sampling
- slit
- signal processing
- gas circuit
- multipoint mode
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
- C12M33/04—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
- C12M33/06—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles for multiple inoculation or multiple collection of samples
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M23/00—Constructional details, e.g. recesses, hinges
- C12M23/02—Form or structure of the vessel
- C12M23/10—Petri dish
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- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
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- Sustainable Development (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
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- General Engineering & Computer Science (AREA)
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The utility model relates to an on-line multi-point type microorganism collecting system which comprises a plurality of samplers, a vacuum pump source, a main air line, branch air lines, flow control and feedback units and a signal processing and controlling unit, wherein the samplers are correspondingly arranged at a plurality of sampling points, the main air line is communicated with the vacuum pump source, the branch air lines are used for communicating the main air line with the samplers respectively, the flow control and feedback units are arranged on the branch air lines and are used for controlling sampling flow of corresponding branch air lines and feeding flow data back to the signal processing and controlling unit in real time, and the signal processing and controlling unit is used for independently processing data fed back by the flow control and feedback units and controlling the sampling of the plurality of samplers. In the on-line multi-point type microorganism collecting system, the same control unit and the same vacuum pump source are adopted to collect microorganisms at a plurality of different positions at the same time; the sampling can be carried out individually or at the same time at each position; the sampling amount at each position can be set independently as required; moreover, the sampling is simple to operate and has high efficiency.
Description
Technical field
The utility model relates to a kind of microorganism acquisition system.
Background technology
Along with the further investigation of biotechnology and the continuous expansion of its Application Areas, Biosafety is more and more paid much attention to.Because the manipulation object of biotechnology all is the organism of microorganism, viable cell and so on, or their recombinant chou, varient, exist and to cause communicate illness, harm operator ' s health so that influence the negative factor of environment, particularly in engineered experimental study, exist the potential hazard that is difficult to predict.In addition, also very strict at pharmaceutical industry to the control requirement of microorganism, so the detection of microorganism is very important.And at present, the microorganism sampler that is used to gather microorganism substantially all is a desk-top instrument, operating main body is that operation interface and collection head connect together, needing personnel to carry instrument measures, yet rising along with the clean environment grade, the step that personnel enter this environment is also complicated more, and personnel frequently pass in and out this environment and cause brokenly the risk of ring also just high more to clean environment, also can work the mischief to operator under the same part hostile environment.In addition, present microorganism sampler is the single-point sampling, promptly uses a sampling thief to sample at a point, is unsuitable for multi-point sampling.
Summary of the invention
Technical problem to be solved in the utility model is to overcome the deficiencies in the prior art, and a kind of online multipoint mode microorganism acquisition system is provided, and it is used in a plurality of different locations and carries out the microorganism collection simultaneously.
For solving the problems of the technologies described above, the utility model is taked following technical scheme:
A kind of online multipoint mode microorganism acquisition system, it comprises gas circuit that correspondence is arranged on a plurality of sampling thiefs of a plurality of sampling points, vacuum pumping source, the main gas circuit that is communicated with the vacuum pumping source, is used for a plurality of sampling thiefs are communicated with main gas circuit respectively, is arranged on flow control and feedback unit, signal processing and control unit on each gas circuit, the sampling flow that described flow control and feedback unit prop up gas circuit to its place is controlled, and data on flows is fed back to described signal processing and control unit in real time; Described signal processing and control unit are independently to described a plurality of flow controls and the feedback unit feedback data is handled and a plurality of sampling thiefs samplings of independent control, described sampling thief comprises sampling head, the culture dish that is arranged at the acquisition port below with acquisition port and slit, culture dish support and the base that is used to support culture dish, and described base is communicated with a gas circuit.
Preferably, described sampling thief and described signal processing and control unit split are provided with, thereby can realize separating of operator and testing environment, avoid operator to the interference of testing environment and hostile environment to health effects.
According to further embodiment of the present utility model: described slit has a plurality of, and a plurality of slit is along the circumferential uniform distribution of described acquisition port.Preferably, described acquisition port is circular, and each described slit is along the radially extension of acquisition port.Upstream at described each slit also is provided with the buffering ramp pan, and the design of described sampling head meets the following conditions:
S
Slit=b*l*t
Wherein:S
Slit The total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number;S
Always The top surface area of expression acquisition port;V
Bump The expression microorganism strikes the speed that culture dish needs;V
Sampling Expression sampling flow velocity.
Above-mentioned buffering ramp pan is tubaeform that the direction towards described slit narrows down gradually from the upper surface of acquisition port.
Because the enforcement of above technical scheme, the utility model compared with prior art has following advantage:
The utility model uses same control unit, and same vacuum pumping source can be implemented in a plurality of different mining sampling points and carry out the microorganism collection simultaneously.A plurality of sampling points can be sampled simultaneously and also can be sampled separately, and the independently setting as required of the sampling quantity of each sampling point, and sampling operation is easy, the efficient height.
Description of drawings
The utility model is described in more detail below in conjunction with specific embodiment.
Fig. 1 is the structural representation according to online multipoint mode microbe sampling of the present utility model system;
Fig. 2 is the structural representation according to sampling thief of the present utility model;
Fig. 3 is the main cross-sectional schematic according to sampling head of the present utility model;
Fig. 4 is an A place enlarged view among Fig. 3;
Fig. 5 is the structural representation according to flow control of the present utility model and feedback unit;
Wherein: 1, sampling head; 10, acquisition port; 11, slit; 12, buffering ramp pan; 2, culture dish; 3, culture dish support; 4, base; B, slit width; 5, flow control and feedback unit; 50, magnetic valve; 51, variable valve; 52, flow sensor; 53, control circuit board; 6, vacuum pumping source; 7, signal processing and control unit; 8, main gas circuit; 9, prop up gas circuit.
Embodiment
As shown in Figure 1, comprise a gas circuit 9 that correspondence is arranged on a plurality of sampling thiefs of a plurality of sampling points, vacuum pumping source 6, the main gas circuit 8 that is communicated with vacuum pumping source 6, is used for a plurality of sampling thiefs are communicated with main gas circuit 8 respectively, be arranged on flow control and feedback unit 5, signal processing and control unit 7 on each gas circuit 9 according to the online multipoint mode microorganism acquisition system of present embodiment.Wherein:
Shown in Fig. 2 to 4, sampling thief comprises sampling head 1, culture dish 2, is used to support the culture dish support 3 and the base 4 of culture dish 2, and the bottom of base 4 is communicated with a gas circuit 9.Base 2 can be fixed according to environment for use, but also the special-purpose trivet of adapted uses flexibly.Sampling head 1 has a plurality of slits 11, and a plurality of slits 11 are along the circumferential uniform distribution of acquisition port 10, each described slit 11 is along the radially extension of acquisition port 10, and is provided with buffering ramp pan 12 in the upstream of each slit 11, and the design of described sampling head meets the following conditions:
S
Slit=b*l*t
Wherein:S
Slit The total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number;S
Always The top surface area of expression acquisition port;V
Bump The expression microorganism strikes the speed that culture dish needs;V
Sampling Expression sampling flow velocity,V
Sampling Numerical value equate with the clean environment laminar velocity.
Satisfy the sampling head of above condition and can realize the isokinetic sampling.During sampling, sampling head 1 top forms the top-down air-flow with clean environment laminar flow constant speed, and after air communication was crossed slit 11, flow velocity increased toV
Bump, Thereby microorganism is effectively impinged upon on the substratum in the culture dish 2.This sampling head design optimization stroke speed, guaranteed biology efficient.Improved the collecting efficiency of the active particle that meets the ISO14698-1 standard; Whether in addition, using the utility model can be easy to pick out is effective microbe, and only the microorganism at the slit location place is effectively, and all the other are local, and then explanation does not collect in gatherer process if there is microorganism to exist, for artificial the interference causes.
As shown in Figure 5, flow control and feedback unit 5 are used for its place is propped up the sampling flow of gas circuit 9 to be controlled, and data on flows is fed back to described signal processing and control unit 7 in real time.Flow control and feedback unit 5 comprise magnetic valve 50, variable valve 51, flow sensor 52 and control circuit board 53, and its setting can be with reference to prior art.
Described signal processing and control unit 7 are independently to described a plurality of flow controls and feedback unit 5 feedback data are handled and a plurality of sampling thiefs samplings of independent control.Signal processing and control unit 1 can Controlling System in the optional position sampling thief sample, each point can be sampled simultaneously and also can be sampled separately, and the sampling quantity of each point can be provided with respectively according to actual needs.Signal processing and control unit 7 is split setting with sampling head 1, so, can realize separating of operator and testing environment, avoid operator to the interference of testing environment and hostile environment to health effects.
To sum up, online multipoint mode microbe sampling of the present utility model system can be implemented in a plurality of different locations and carry out the microorganism collection simultaneously, and its sampling head adopts constant speed principle design, can not break the laminar flow of ring clean environment; Use split-type design, can avoid personnel operation that sampling is caused human intervention.
More than the utility model has been done detailed description; its purpose is to allow the personage that is familiar with this art can understand content of the present utility model and is implemented; can not limit protection domain of the present utility model with this; all equivalences of doing according to spirit of the present utility model change or modify, and all should be encompassed in the protection domain of the present utility model
Claims (7)
1. online multipoint mode microorganism acquisition system, it is characterized in that: described acquisition system comprises that correspondence is arranged on a plurality of sampling thiefs of a plurality of sampling points, vacuum pumping source (6), the main gas circuit (8) that is communicated with vacuum pumping source (6), be used for gas circuit (9) that described a plurality of sampling thiefs are communicated with described main gas circuit (8) respectively, be arranged on flow control and feedback unit (5) on described each gas circuit (9), signal processing and control unit (7), the sampling flow that described flow control and feedback unit (5) prop up gas circuit (9) to its place is controlled, and data on flows is fed back to described signal processing and control unit (7) in real time; Described signal processing and control unit (7) are independently to described a plurality of flow controls and feedback unit (5) feedback data is handled and the described a plurality of sampling thiefs samplings of independent control, described sampling thief comprise have acquisition port (10) and slit (11) sampling head (1), be arranged at described acquisition port (10) below culture dish (2), be used to support the culture dish support (3) and the base (4) of culture dish (2), described base (4) is communicated with described gas circuit (4).
2. online multipoint mode microorganism acquisition system according to claim 1 is characterized in that: described sampling head (1) and described signal processing and control unit (7) split setting.
3. online multipoint mode microorganism acquisition system according to claim 1, it is characterized in that: described slit (11) has a plurality of, and a plurality of slit (11) is along the circumferential uniform distribution of described acquisition port (10).
4. online multipoint mode microbe sampling according to claim 3 system, it is characterized in that: described acquisition port (10) is for circular, and each described slit (11) is along the radially extension of described acquisition port (10).
5. online multipoint mode microbe sampling according to claim 3 system, it is characterized in that: be provided with buffering ramp pan (12) in the upstream of described each slit (11), the design of described sampling head meets the following conditions:
S
Slit=b*l*t
Wherein: S
SlitThe total area of representing described a plurality of slits; B represents slit width; L represents slit length; T represents slot number; S
AlwaysThe top surface area of expression acquisition port; V
BumpThe expression microorganism strikes the speed that culture dish needs; V
SamplingExpression sampling flow velocity.
6. online multipoint mode microbe sampling according to claim 5 system is characterized in that: described buffering ramp pan (12) is tubaeform that the direction towards described slit (11) narrows down gradually from the upper surface of described acquisition port (10).
7. online multipoint mode microbe sampling according to claim 1 system, it is characterized in that: the material of described sampling head (1), base (4) is the 316L stainless steel.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120111495 CN202063918U (en) | 2011-04-15 | 2011-04-15 | On-line multi-point type microorganism collecting system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201120111495 CN202063918U (en) | 2011-04-15 | 2011-04-15 | On-line multi-point type microorganism collecting system |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN202063918U true CN202063918U (en) | 2011-12-07 |
Family
ID=45057787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201120111495 Expired - Lifetime CN202063918U (en) | 2011-04-15 | 2011-04-15 | On-line multi-point type microorganism collecting system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN202063918U (en) |
-
2011
- 2011-04-15 CN CN 201120111495 patent/CN202063918U/en not_active Expired - Lifetime
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| ASS | Succession or assignment of patent right |
Owner name: SUZHOU SUJING INSTRUMENT AUTOMATIC CONTROL EQUIPME Effective date: 20121214 Owner name: JIANGSU SUJING GROUP CO. LTD. Free format text: FORMER OWNER: SUZHOU SUJING INSTRUMENT AUTOMATIC CONTROL EQUIPMENT CO., LTD. Effective date: 20121214 |
|
| C41 | Transfer of patent application or patent right or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20121214 Address after: 215122 Suzhou Industrial Park, Jiangsu, the only new road, No. 2 Patentee after: Jiangsu Sujing Group Co., Ltd. Patentee after: Suzhou Sujing Group Automation Instrument Equipment Corporation Address before: 215122 Suzhou Industrial Park, Suzhou Province, the only new road, No. 2, Jiangsu Patentee before: Suzhou Sujing Group Automation Instrument Equipment Corporation |
|
| CX01 | Expiry of patent term | ||
| CX01 | Expiry of patent term |
Granted publication date: 20111207 |