CN201395600Y - Integrated cell culture device with separable substrate - Google Patents
Integrated cell culture device with separable substrate Download PDFInfo
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- CN201395600Y CN201395600Y CN2009201264961U CN200920126496U CN201395600Y CN 201395600 Y CN201395600 Y CN 201395600Y CN 2009201264961 U CN2009201264961 U CN 2009201264961U CN 200920126496 U CN200920126496 U CN 200920126496U CN 201395600 Y CN201395600 Y CN 201395600Y
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- substrate
- cell culture
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- cell
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Abstract
The utility model discloses an integrated cell culture device with a separable substrate, which comprises a substrate, wherein the substrate is provided with at least a cell placing block. The integrated cell culture device with the separable substrate further comprises an observation hole cover whose upper end is closed, wherein the inner portion of the observation hole cover is uniformly provided with observation cavities whose number is same to the cell placing blocks, the lower portion is embedded in a fixed shallow slot, and the corresponding cell placing blocks are sealed in the inner portion of the observation cavities. The substrate and the observation hole cover are fixedly connected in a separable mode, through arranging cell culture blocks, the substrate and the observation holecover can be conveniently separated after completing the cell culture, thereby being capable of directly using the substrate in next various histochemistry tests, no needing to use tedious operationsto take out a slide bearing cells from a culture dish as before, saving manpower, increasing the efficiency, and greatly reducing the disk of takeout operation of the cells.
Description
Technical field
The utility model relates to a kind of integral type cell culture apparatus with separable substrate.
Background technology
Modern biotechnology be it is generally acknowledged and comprised genetic engineering technique, cell engineering, enzyme engineering technology and fermentation engineering, and the development of these technology nearly all has substantial connection with cell cultures, particularly in the development of field of medicaments, cell cultures has more special effect and value.Much realize in researching and producing process such as genetically engineered drug or vaccine by cell cultures.Recombinant hepatitis b vaccine much is as carrier with Chinese hamster ovary celI; Especially from cell cultures not, authentic monoclonal antibody is realized by cell cultures fully in the cell engineering, and both having made is that the genetic engineering antibody of present develop rapidly also be unable to do without cell cultures.The gene therapy of extremely paying attention to, treated autologous cell also will could realize that it is also closely related with cell cultures that fermentation engineering and enzyme engineering have through cell cultivation process.In a word, cell cultures has played very crucial central role in the development of whole biotechnology industry.
Existing cell cultures is mainly finished in cultivating vessel, as shown in Figure 1, commonly used have 12 orifice plates, 24 orifice plates etc. and a supporting cultivation pool, after cell cultures is finished, in the time of will carrying out next step test, the slide that needs to carry cell carefully takes out from cultivate vessel, because hole is comparatively narrow and small,, just cause slide to topple easily if careless slightly when taking out operation, produce and pollute, thereby the effort of culturing cell in early stage is destroyed overnight.
The utility model content
In view of this, the purpose of this utility model provides a kind of integral type cell culture apparatus with separable substrate, cell cultures and groupization test all can be concentrated on the same substrate to finish, and has overcome the existing apparatus function singleness, complex operation, the shortcoming that error rate is high.
Integral type cell culture apparatus with separable substrate of the present utility model comprises substrate, and described substrate is provided with at least one cell and places piece, is provided with fixedly shallow slot around the described cell placement piece;
Also comprise the observation port lid, the upper end closed of described observation port lid, the inside of observation port lid evenly is provided with quantity and cell and places the identical observation chamber of piece, and the bottom of described observation chamber is embedded in fixedly in the shallow slot and by observing chamber that corresponding cell placement piece is enclosed in the observation chamber interior;
Described substrate is detachable fixedlying connected with observing between the port lid;
Further, the mode of establishing by spiral shell between described substrate and the observation chamber achieves a fixed connection;
Further, evenly be provided with screw on the periphery of described substrate, Dui Ying screw rod evenly is arranged on the bottom of observation chamber with it;
Further, the top of described observation chamber seals by closing cap, and a side of described closing cap is provided with feature portion;
Further, each limit, the bottom surface of described observation chamber is provided with sealed strip, and the length of described sealed strip is corresponding with the length of fixing shallow slot;
Further, described substrate is provided with the identifier that cell is placed piece;
Further, described substrate is a slide, and described observation port lid and closing cap adopt transparent material to make.
The beneficial effects of the utility model are:
1. by the cell cultures piece is set on substrate, after cell cultures is finished, this substrate can separate easily with the observation port lid, thereby substrate can be directly used in various groups of change tests carrying out next step, and needn't be as in the past, the slide that needs to adopt comparatively loaded down with trivial details operation could will carry cell takes out from cultivate vessel, thereby has saved manpower, improved efficient, and greatly reduced cell and taken out the risk of operating;
2. pass through the design of observation port lid, can be in the process of cell cultures, the pair cell situation is observed at any time, is convenient to adopt various measures; In addition,, can the cell in cultivating be sealed preferably, avoid cell contamination by the design of observation chamber;
3. the mode of establishing by spiral shell will observe port lid and substrate link together, and not only play the fixed effect, also be convenient to both are separated easily, in addition, be provided with sealed strip at both connecting portion, can play the effect that strengthens sealing effectiveness;
4. by feature portion being set and the fast identifier of cell cultures being set, can help the clearly different cell cultures piece of identification of operator on closing cap, avoid different cell cultures is obscured soon at substrate.
The utility model design is ingenious, and effect is obvious, and is easy and simple to handle, is applicable to that each hospital, university, scientific research institutions and individual in the use of cell culture test link, are worth promoting.
Other advantages of the present utility model, target, to set forth in the following description to a certain extent with feature, and to a certain extent, based on being conspicuous to those skilled in the art, perhaps can from practice of the present utility model, obtain instruction to investigating hereinafter.Target of the present utility model and other advantages can realize and obtain by following specification sheets and claims.
Description of drawings
In order to make the purpose of this utility model, technical scheme and advantage clearer, below in conjunction with accompanying drawing the utility model is described in further detail, wherein:
Fig. 1 is existing 12 AND DEWATERING FOR ORIFICE STRUCTURE synoptic diagram;
Fig. 2 is a substrate structure synoptic diagram of the present utility model;
Fig. 3 is an observation port lid structural representation of the present utility model;
Fig. 4 is a closing cap structural representation of the present utility model;
Fig. 5 is the connection diagram of each parts of the utility model.
Embodiment
Hereinafter with reference to accompanying drawing, preferred embodiment of the present utility model is described in detail.Should be appreciated that preferred embodiment only for the utility model is described, rather than in order to limit protection domain of the present utility model.
As shown in Figure 2, on the slide of a 185 * 85mm, be provided with four cell cultures pieces 11 that are of a size of 75 * 25mm, cell cultures piece 11 uniform distribution on slide, between the cell cultures piece 11 that links, process many fixedly shallow slots 12 by frosting technology, fixedly the width of shallow slot 12 is about 5mm, working depth is about 1～2mm, around slide, evenly process six screws 13, so far, substrate 1 completes, and this substrate 1 is used for the cultivation of cell, and can be used as the cell slide glass use of next step groupization test.
As shown in Figure 3, the utility model also comprises observation port lid 2, the inside of observation port lid 2 evenly is provided with the identical observation chamber 21 of quantity and cell placement piece 11, the upper end of observation port lid 2 is sealed by closing cap 23, and the bottom is embedded in the fixing shallow slot 12 and by observation chamber 21 cell of correspondence is placed piece 11 and is enclosed in and observes chamber 21 inside; Each limit, bottom surface of observation chamber 21 is provided with sealed strip, and the length of sealed strip is corresponding with the length of fixing shallow slot 12, by the sealing bar, and acquisition better seal in the time of can guaranteeing to observe port lid 2 to fixedly connected with substrate 1.
Bottom at the observation chamber evenly is provided with the screw rod 22 that is used for screw 13 bolts.
As shown in Figure 4, at the head and the tail of being convenient to distinguish this device, make things convenient for pair cell to place the identification of piece, and device is attractive in appearance, is provided with feature portion in a side of the end face of closing cap 23, this feature portion gets by two angle circular arcizations that will be positioned on the same minor face;
Obscure for avoiding producing between the cell placement piece, substrate 1 is provided with the identifier that cell is placed piece 11.
For ease of observing, observation port lid 2 and closing cap 23 adopt transparent material to make, as PET, PVC etc.
As shown in Figure 5, the utility model in use, now need cultured cells to be filled on the cell cultures piece 11, to observe screw 13 insertions on screw rod 22 alignment substrate 1 on the port lid 2 then, use nut screwing clamping then, realize sealing, in the process of cell cultures, if needed, can open closing cap 23;
After treating that cell cultures is finished, will observe on port lid 2 and the substrate 1 and separating, substrate 1 can be directly used in next step various tests.
By this device, avoided the trouble of using traditional cell cultures orifice plate the cell cultures slide need be taken out from culture hole, be highly suitable for each hospital, university, scientific research institutions and individual in the use of cell culture test link, be worth promoting.
Explanation is at last, above embodiment is only unrestricted in order to the explanation the technical solution of the utility model, although the utility model is had been described in detail with reference to preferred embodiment, those of ordinary skill in the art is to be understood that, can make amendment or be equal to replacement the technical solution of the utility model, and not breaking away from the aim and the scope of the technical program, it all should be encompassed in the middle of the claim scope of the present utility model.
Claims (7)
1. with the integral type cell culture apparatus of separable substrate, it is characterized in that: comprise substrate (1), described substrate (1) is provided with at least one cell and places piece (11), is provided with fixedly shallow slot (12) around the described cell placement piece (11);
Also comprise observation port lid (2), the upper end closed of described observation port lid (2), the inside of described observation port lid (2) evenly is provided with quantity and places the identical observation chamber (21) of piece (11) with cell, and the bottom of described observation chamber (21) is embedded in fixedly and also is enclosed in observation chamber (21) inside by observing chamber (21) that the cell of correspondence is placed piece (11) in the shallow slot (12);
Described substrate (1) is detachable fixedlying connected with observing between the port lid (2).
2. the integral type cell culture apparatus with separable substrate according to claim 1 is characterized in that: the mode of establishing by spiral shell between described substrate (1) and the observation chamber (21) achieves a fixed connection.
3. the integral type cell culture apparatus with separable substrate according to claim 2 is characterized in that: evenly be provided with screw (13) on the periphery of described substrate (1), Dui Ying screw rod (22) evenly is arranged on the bottom of observation chamber (21) with it.
4. according to claim 1 or 2 or 3 described integral type cell culture apparatus with separable substrate, it is characterized in that: the top of described observation port lid (2) seals by closing cap (23), and a side of described closing cap (23) end face is provided with feature portion.
5. the integral type cell culture apparatus with separable substrate according to claim 4 is characterized in that: each limit, bottom surface of described observation chamber (21) is provided with sealed strip, and the length of described sealed strip is corresponding with the length of fixing shallow slot (24).
6. the integral type cell culture apparatus with separable substrate according to claim 5 is characterized in that: described substrate (1) is provided with the identifier that cell is placed piece (11).
7. the integral type cell culture apparatus with separable substrate according to claim 6 is characterized in that: described substrate (1) is a slide, and described observation port lid (2) and closing cap (23) adopt transparent material to make.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201264961U CN201395600Y (en) | 2009-02-27 | 2009-02-27 | Integrated cell culture device with separable substrate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009201264961U CN201395600Y (en) | 2009-02-27 | 2009-02-27 | Integrated cell culture device with separable substrate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN201395600Y true CN201395600Y (en) | 2010-02-03 |
Family
ID=41618103
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2009201264961U Expired - Fee Related CN201395600Y (en) | 2009-02-27 | 2009-02-27 | Integrated cell culture device with separable substrate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN201395600Y (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226151A (en) * | 2011-05-13 | 2011-10-26 | 刘小龙 | Removable cell imaging device |
| CN102226150A (en) * | 2011-05-13 | 2011-10-26 | 刘小龙 | Cell cultivating apparatus used for imaging living cell |
| CN102676369A (en) * | 2011-12-19 | 2012-09-19 | 河南科技大学 | Evaluation model for zinc biological effectiveness in foods and establishment method thereof |
-
2009
- 2009-02-27 CN CN2009201264961U patent/CN201395600Y/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102226151A (en) * | 2011-05-13 | 2011-10-26 | 刘小龙 | Removable cell imaging device |
| CN102226150A (en) * | 2011-05-13 | 2011-10-26 | 刘小龙 | Cell cultivating apparatus used for imaging living cell |
| CN102676369A (en) * | 2011-12-19 | 2012-09-19 | 河南科技大学 | Evaluation model for zinc biological effectiveness in foods and establishment method thereof |
| CN102676369B (en) * | 2011-12-19 | 2013-12-25 | 河南科技大学 | Evaluation model for zinc biological effectiveness in foods and establishment method thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20100203 Termination date: 20100227 |