CN212357269U - Cell oxygen deficiency culture apparatus - Google Patents

Abstract

The utility model relates to a cell culture device field specifically discloses a cell oxygen deficiency culture apparatus, including the box, be equipped with a plurality of cell culture boxes in the box, be equipped with a plurality of culture dishes in the cell culture box and place the subassembly, the culture dish is placed the subassembly and is included lateral wall open-ended half tube-shape culture dish and place the piece, fixedly connected with culture dish limiting plate in the culture dish is placed, along every the upper edge department of culture dish limiting plate has seted up the spacing groove, sliding connection has the spacing piece of arc in the spacing groove. The utility model discloses a set up the culture dish and place the subassembly and make the culture dish can stably place alone, avoided the damage and the pollution in the use.

Description

Cell oxygen deficiency culture apparatus

Technical Field

The utility model relates to a cell culture device field specifically discloses a cell oxygen deficiency culture apparatus.

Background

Cell culture (cell culture) refers to a method for simulating in vivo environment (sterility, proper temperature, pH value, certain nutritional conditions and the like) in vitro to enable the cells to survive, grow and reproduce and maintain main structures and functions. Cell culture is also called cell cloning technology, and the formal term in biology is cell culture technology.

Cell oxygen deficiency culture in the cell culture is the important means in the research work, and the cell oxygen deficiency incubator that uses in laboratory at present only has a box, can't carry out the molding of same batch of cell simultaneously, and used culture dish generally stacks each other at the cell culture in-process, and operation process leads to easy landing, causes the pollution of culture dish damage and cell, consequently, the utility model provides a cell oxygen deficiency culture apparatus.

SUMMERY OF THE UTILITY MODEL

The technical insufficiency that exists to the aforesaid, the utility model aims at providing a reducing mechanism of edible fungi culture material reed solves the problem pointed out among the above-mentioned background art.

In order to solve the technical problem, the utility model adopts the following technical scheme:

the utility model provides a cell oxygen deficiency culture apparatus, include:

the cell culture device comprises a box body, a plurality of cell culture boxes and a plurality of culture dish placing assemblies, wherein the plurality of cell culture boxes are arranged in the box body;

the subassembly is placed to the culture dish includes a plurality of vertical fixed connection's in proper order culture dish limiting plate, along every the spacing groove has been seted up to the top edge department of culture dish limiting plate, sliding connection has the spacing piece of arc in the spacing groove.

Preferably, a plurality of partition plates are arranged in the box body and are parallel to the bottom wall of the box body, and the cell culture box is fixedly connected to the partition plates and the bottom wall of the box body.

Preferably, the rear wall of the cell culture box is tightly attached to the rear wall of the box body, a first air inlet for introducing first air, a second air inlet for introducing second air and a third air inlet for introducing third air are formed in the rear wall of the cell culture box, the first air inlet, the second air inlet and the third air inlet penetrate through the rear wall of the box body and are connected with air pipelines, and the other end of each air pipeline is connected with an air source.

Preferably, the first gas is oxygen, the second gas is carbon dioxide, and the third gas is nitrogen.

Preferably, a first automatic control valve, a second automatic control valve and a third automatic control valve are respectively arranged on the gas pipelines connected with the first gas inlet, the second gas inlet and the third gas inlet.

Preferably, the cell culture box is internally provided with a first sensor, a second sensor and a third sensor, wherein the first sensor is used for detecting the first gas, the second sensor is used for detecting the second gas, the third sensor is used for detecting the third gas, the first sensor is electrically connected with the first automatic control valve, the second sensor is electrically connected with the second automatic control valve, and the third sensor is electrically connected with the third automatic control valve.

Preferably, cell culture box lateral wall hinge connection has the box door, be equipped with the display on the box door outer wall, the display with first sensor, second sensor and third sensor electric connection, be equipped with first gate on the box door and close.

Preferably, the box body is hinged with a box door, the box door is provided with a transparent window corresponding to the position of the display, and one side of the opening of the box door is provided with a second door closer.

The beneficial effects of the utility model reside in that: according to the cell hypoxia culture device, the plurality of cell culture boxes are arranged in the box body, and the first sensor, the second sensor, the third sensor, the first automatic control valve, the second automatic control valve, the third automatic control valve and the display are arranged in the cell culture boxes, so that the purpose of independently controlling the gas concentration in each cell culture box is achieved, finally, the molding test of a plurality of groups of cells at different time points can be carried out by only using one box body, a large amount of time is saved, and the test accuracy is improved; still place the subassembly through setting up the culture dish in cell culture box and realized that every culture dish separately places, carry on spacingly to the culture dish through the culture dish limiting plate, prevent to drop.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic structural view of a cell hypoxia culture apparatus according to an embodiment of the present invention;

FIG. 2 is a schematic diagram of the cell culture cassette of FIG. 1;

FIG. 3 is a schematic view of a portion of the plate placement assembly of FIG. 2;

FIG. 4 is a schematic view of the connection structure of the cell culture cassette of FIG. 1 with a gas source;

FIG. 5 is a schematic view of a portion of the structure of FIG. 4;

FIG. 6 is a schematic view of the structure of the door of FIG. 2;

description of reference numerals: the automatic culture device comprises a box body 1, a box door 2, a cell culture box 3, a first air inlet 4, a second air inlet 5, a third air inlet 6, a box door 301, a culture dish placing assembly 302, a first sensor 303, a second sensor 304, a third sensor 305, a display 3011, a culture dish limiting plate 3021, a limiting groove 3022 and an arc limiting sheet 3023.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

As shown in fig. 1 to 6, a cell anoxic culture apparatus includes:

the cell culture device comprises a box body 1, wherein a plurality of cell culture boxes 3 are arranged in the box body 1, and a plurality of culture dish placing assemblies 302 are fixedly connected in the cell culture boxes 3;

the subassembly 302 is placed to the culture dish includes that lateral wall open-ended semi-cylindrical culture dish places the piece, the opening horizontal length that the culture dish placed the piece is greater than the culture dish external diameter, a plurality of culture dish limiting plates 3021 of fixedly connected with in the culture dish placed the piece, along every spacing groove 3022 has been seted up to culture dish limiting plate 3021's top edge department, sliding connection has the spacing piece of arc 3023 in the spacing groove 3022, the spacing piece 3023 arc length of arc is greater than the groove length of third spacing groove 3022, is less than half the groove length of spacing groove 3022, the spacing piece 3023 of arc can along the spacing groove 3022 slides extremely arbitrary position in the spacing groove 3022.

During the use, on putting into the culture dish limiting plate 3021 in the cell culture box 3 respectively with the culture dish that has the cell of cultivateing, slide the spacing piece 3023 of arc to spacing groove 3022 central point in spacing groove 3022, carry on spacingly to the culture dish, after the cultivation, slide the spacing piece 3023 of arc to one side of spacing groove 3022, make it not block the culture dish, then slowly paste the culture dish limiting plate 3021 with the culture dish and take out.

In this embodiment, a plurality of partition boards are arranged in the box body 1, the partition boards are parallel to the bottom wall of the box body 1, the cell culture box 3 is fixedly connected to the partition boards and the bottom wall of the box body 1, and the function of the partition boards is set to provide support for the cell culture box 3, so that the internal space of the box body is fully utilized.

In this embodiment, the back wall of the cell culture box 3 is tightly attached to the back wall of the box body 1, the back wall of the cell culture box 3 is provided with a first air inlet 4 for introducing a first gas, a second air inlet 5 for introducing a second gas and a third air inlet 6 for introducing a third gas, the first air inlet 4, the second air inlet 5 and the third air inlet 6 all penetrate through the back wall of the box body 1 and are connected with gas pipelines, the other end of the gas pipeline is connected with a gas source, the first air inlet 4 is correspondingly connected with a gas pipeline a, the second air inlet 5 is correspondingly connected with a gas pipeline b, the third air inlet 6 is correspondingly connected with a gas pipeline c, the gas pipeline a, the gas pipeline b and the gas pipeline c are respectively and correspondingly connected with gas sources containing the first gas, the second gas and the third gas, the first air inlet 4, the second air inlet 5 and the third air inlet 6 are arranged for introducing gas into the cell culture box 3, providing an anoxic environment for the anoxic culture of the cells.

The first gas is oxygen, the second gas is carbon dioxide, the third gas is nitrogen, and cell hypoxia conditions required by experiments are set by controlling the relative content of the oxygen, the carbon dioxide and the nitrogen.

Be provided with first automatic control valve on the gas pipeline a, be provided with second automatic control valve on the gas pipeline b, be equipped with third automatic control valve on the gas pipeline c, realize the automatic control valve switch through first automatic control valve, second automatic control valve and third automatic control valve to realize control the gas concentration in the cell culture box 3 uses manpower sparingly and time.

The cell culture box 3 is also internally provided with a first sensor 303, a second sensor 304 and a third sensor 305, the first sensor 303 is used for detecting the first gas, the second sensor 304 is used for detecting the second gas, the third sensor 305 is used for detecting the third gas, the first sensor 303 is electrically connected with the first automatic control valve, the second sensor 304 is electrically connected with the second automatic control valve, the third sensor 305 is electrically connected with the third automatic control valve, the first sensor 303 senses the content of the first gas, when a set value is reached, the first automatic control valve is closed, so that the first gas does not enter the cell culture box 3 any more, the air inflow control of the second gas and the third gas is correspondingly realized, and finally, the independent control of the anoxic environment in each cell culture box 3 is realized, the experimental personnel can culture the cells respectively according to the molding time points when molding the cells at different time points, so that the experimental result is more accurate.

3 lateral walls of cell culture box hinge connection have box door 301, box door 301 inboard is equipped with the sealing layer, be equipped with display 3011 on the box door 301 outer wall, the display with first sensor 303, second sensor 304 and third sensor 305 electric connection, be equipped with first gate on the box door 301 and close, the setting up of display 3011 makes the experimenter observe the concentration of first gas, second gas and third gas in the cell culture box 3 at any time.

Hinge connection has chamber door 2 on the box 1, be equipped with on chamber door 2 with the transparent window that display 3011 position corresponds, 2 opening one side of chamber door are equipped with the second door and close, first door close with the second door closes the back, can observe the information on the display 3011 through transparent window.

It will be apparent to those skilled in the art that various changes and modifications may be made without departing from the spirit and scope of the invention. Thus, if such modifications and variations of the present invention fall within the scope of the claims and their equivalents, the present invention is also intended to include such modifications and variations.

Claims (8)

1. A cell hypoxia culture device, comprising:

the cell culture device comprises a box body (1), wherein a plurality of cell culture boxes (3) are arranged in the box body (1), and a plurality of culture dish placing assemblies (302) are arranged in the cell culture boxes (3);

subassembly (302) is placed including the half tube-shape culture dish of lateral wall open-ended and places the piece to the culture dish, a plurality of culture dish limiting plates of fixedly connected with (3021) in the culture dish is placed, along every spacing groove (3022) have been seted up to the top edge department of culture dish limiting plate (3021), sliding connection has arc spacing piece (3023) in spacing groove (3022).

2. The anoxic cell culture apparatus according to claim 1, wherein a plurality of partition plates are arranged in the box body (1), the partition plates are parallel to the bottom wall of the box body (1), and the cell culture box (3) is fixedly connected to the partition plates and the bottom wall of the box body (1).

3. The hypoxia cell culture device according to claim 2, wherein the rear wall of the cell culture box (3) is closely attached to the rear wall of the box body (1), the rear wall of the cell culture box (3) is provided with a first air inlet (4) for introducing a first gas, a second air inlet (5) for introducing a second gas and a third air inlet (6) for introducing a third gas, the first air inlet (4), the second air inlet (5) and the third air inlet (6) penetrate through the rear wall of the box body (1) and are connected with a gas pipeline, and the other end of the gas pipeline is connected with a gas source.

4. The apparatus of claim 3, wherein the first gas is oxygen, the second gas is carbon dioxide, and the third gas is nitrogen.

5. The anoxic cell culture apparatus according to claim 3, wherein the gas pipelines connected with the first gas inlet (4), the second gas inlet (5) and the third gas inlet (6) are respectively provided with a first automatic control valve, a second automatic control valve and a third automatic control valve.

6. The anaerobic cell culture device according to claim 3, wherein a first sensor (303), a second sensor (304) and a third sensor (305) are further arranged inside the cell culture box (3), the first sensor (303) is used for detecting the first gas, the second sensor (304) is used for detecting the second gas, the third sensor (305) is used for detecting the third gas, the first sensor (303) is electrically connected with the first automatic control valve, the second sensor (304) is electrically connected with the second automatic control valve, and the third sensor (305) is electrically connected with the third automatic control valve.

7. The device for the anoxic culture of cells according to claim 6, wherein the side wall of the cell culture box (3) is hinged with a box door (301), a display (3011) is arranged on the outer wall of the box door (301), the display is electrically connected with the first sensor (303), the second sensor (304) and the third sensor (305), and a first door is arranged on the box door (301).

8. The cell oxygen-deficient culture device according to claim 7, wherein a box door (2) is hinged on the box body (1), a transparent window corresponding to the display (3011) is arranged on the box door (2), and a second door is arranged on one side of the opening of the box door (2).

Images