CN214937542U

CN214937542U - Cell culture case that can carry out oxygen deficiency experiment

Info

Publication number: CN214937542U
Application number: CN202121088717.8U
Authority: CN
Inventors: 周吉; 张涵泳; 许梦畅; 唐彤
Original assignee: Changsha Medical University
Current assignee: Changsha Medical University
Priority date: 2021-05-20
Filing date: 2021-05-20
Publication date: 2021-11-30
Anticipated expiration: 2031-05-20

Abstract

The utility model discloses a cell incubator capable of carrying out an oxygen deficiency experiment, which comprises a box body, a box door and a plurality of layers of shelving plates arranged in the box body, wherein the front side of the top of the box body is provided with a control module; the box body is provided with a ventilation pipe with a blower and a one-way air outlet, the ventilation pipe is connected with a three-way valve, a first pipeline interface of the three-way valve is connected with the ventilation pipe, a second pipeline interface and a third pipeline interface of the three-way valve are respectively connected with a nitrogen source and a clean air source, the air source communicated with the ventilation pipe is controlled through the adjustment of the three-way valve, the gas environment in the box body can be changed into the anaerobic or aerobic condition, and therefore the experiments of aerobic culture and anaerobic culture in the cell culture box are carried out; correspondingly, a nitrogen concentration sensor is arranged on the inner wall of the box body and electrically connected with the control module so as to monitor the change of the nitrogen concentration in the box body.

Description

Cell culture case that can carry out oxygen deficiency experiment

Technical Field

The utility model relates to the technical field of biological equipment, in particular to a cell culture box capable of carrying out an oxygen deficiency experiment.

Background

The cell incubator is a device for culturing cells or tissues in vitro by simulating and forming a growth environment similar to the cells or tissues in an organism in an incubator body, is an instrument for culturing the cells and the tissues, and is key equipment necessary for developing biology, cytology, immunology, oncology, genetics and bioengineering.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is to overcome current defect, provide a cell culture case that can carry out the oxygen deficiency experiment, can carry out the switching of anaerobic or oxygen deficiency environment, need not additionally to arrange cell culture in a sealed bag that is full of nitrogen gas, labour saving and time saving can effectively solve the problem in the background art.

In order to achieve the above object, the utility model provides a following technical scheme:

a cell culture box capable of carrying out an oxygen deficiency experiment comprises a box body, a box door and a plurality of layers of shelving plates arranged in the box body, wherein a control module is arranged on the front side of the top of the box body;

the box body is provided with a ventilation pipe with a blower and a one-way air outlet, the ventilation pipe is connected with a three-way valve, a first pipeline interface of the three-way valve is connected with the ventilation pipe, a second pipeline interface and a third pipeline interface of the three-way valve are respectively connected with a nitrogen source and a clean air source, the air source communicated with the ventilation pipe is controlled through the adjustment of the three-way valve, the gas environment in the box body can be changed into the anoxic or aerobic condition, and therefore the tests of aerobic culture and anoxic culture in the cell culture box are carried out; correspondingly, a nitrogen concentration sensor is arranged on the inner wall of the box body and is electrically connected with the control module so as to monitor the change of the nitrogen concentration in the box body;

the box door is a vertical hinged door and is hinged at the opening position of the box body, and the box door is a sealing door.

Further, still include the gyro wheel, the gyro wheel is installed in the bottom of box, and the gyro wheel is convenient for remove the box.

Further, still include the attacker, the attacker is installed in the end outside that opens and shuts of vertical hinged door, and the attacker is ABS attacker, and the attacker conveniently carries out the opening and closing operation to the chamber door.

Furthermore, a magnetic sealing gasket is arranged at the joint of the box door and the opening of the box body, so that the sealing performance of the interior of the box body when the box door is closed is ensured.

Further, still include the active carbon filter screen, the active carbon filter screen sets up in the breather pipe, and the active carbon filter screen is arranged in filtering the dust in the air, has guaranteed the inside dustless environment of box.

Compared with the prior art, the beneficial effects of the utility model are that: the cell culture box has the following advantages:

1. in the process of air exchange, whether nitrogen or carbon dioxide in the box body meets the requirements can be judged by observing the concentration change condition on the display screen, if the number which represents the nitrogen concentration on the display screen is increased after a period of time in the process of filling nitrogen, the box body can be judged to be filled with nitrogen when the number does not change, and then the carbon dioxide concentration in the cell incubator is adjusted by filling carbon dioxide through the original carbon dioxide source of the cell incubator, so that the carbon dioxide concentration reaches 5% of the conventional requirement; similarly, when filling air, the nitrogen concentration figure diminishes through a period of time, when no longer changing, exactly when the air was exhausted nitrogen gas, can close air-blower and air supply this moment, and carbon dioxide is filled to the original carbon dioxide source of rethread cell culture case and is adjusted the carbon dioxide concentration in the cell culture case, makes the carbon dioxide concentration reach the 5% concentration of conventional requirement. When the aerobic/anoxic cell culture test is carried out, the cell culture is not required to be additionally arranged in a sealed bag filled with nitrogen, so that time and labor are saved, and the use requirement is convenient to meet.

2. The dust in the air is filtered through the activated carbon filter screen, so that the dust-free environment in the box body is ensured;

3. the utility model discloses need not the frequent switch door, as long as fill condition such as air or nitrogen gas humidity, temperature in the cell culture case and satisfy the requirement, just can reduce external environment to the influence of humidity, temperature in the cell culture case, just also need not frequent temperature regulation and humidity, make the cell quality of cultivation higher like this.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic side view of the present invention.

In the figure: the device comprises a box body 1, a placing plate 2, rollers 3, a nitrogen concentration sensor 4, a buckle 5, a control module 6, a box door 7, a magnetic sealing gasket 8, an active carbon filter screen 9 and a three-way valve 10.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-2, the present embodiment provides a technical solution: a cell culture box capable of carrying out an oxygen deficiency experiment comprises a box body 1, a box door 7 and a plurality of layers of shelving plates 2 arranged in the box body 1, wherein a control module 6 is arranged on the front side of the top of the box body 1;

be equipped with the scavenge pipe of taking the air-blower and the one-way gas outlet of giving vent to anger on the box 1, the scavenge pipe is connected with three-way valve 10, the first pipeline interface connection scavenge pipe of three-way valve 10, nitrogen source and clean air source are connected respectively to the second pipeline interface and the third pipeline interface of three-way valve 10, the air supply of adjusting control scavenge pipe switch-on through three-way valve 10 can change the gaseous environment in the box 1 for the condition of oxygen deficiency (nitrogen gas + 5% carbon dioxide) or aerobic (air + 5% carbon dioxide). When an anoxic test is required, opening a nitrogen source to fill nitrogen into the cell culture box, adjusting the concentration of carbon dioxide in the cell culture box to be 5% of the conventional concentration by using the carbon dioxide source of the original cell culture box after the nitrogen source is filled with the nitrogen, and carrying out the anoxic culture test on the cells under the condition; after the oxygen deficiency test is finished, air of a clean air source is discharged into the cell culture box to exhaust nitrogen from the air outlet, and then the concentration of carbon dioxide in the cell culture box is regulated to 5% of the conventional concentration by a carbon dioxide source of the original cell culture box; the concentration of carbon dioxide is detected by a carbon dioxide detector, so that the conditions of aerobic culture and anoxic culture in a cell culture box are met; correspondingly, a nitrogen concentration sensor 4 is arranged on the inner wall of the box body 1, and the nitrogen concentration sensor 4 is electrically connected with the control module 6 so as to monitor the change of the nitrogen concentration in the box body 1;

in the process of taking a breath, can judge whether nitrogen gas or air are full of in the box 1 through observing the concentration change on the display screen, if fill the nitrogen gas in-process, the figure that represents nitrogen gas concentration on the display screen passes through the grow of a period of time, when no longer changing, can judge that nitrogen gas has been full of box 1, and the same reason, when filling the air, the figure of nitrogen gas concentration is through the diminish of a period of time, when no longer changing, when exactly the air is full of. After the cell culture box is filled with nitrogen or air, the air blower and the air source can be closed, and the carbon dioxide concentration in the cell culture box is adjusted by filling carbon dioxide into the original carbon dioxide source of the cell culture box, so that the adjustment is convenient, the cell culture is not required to be additionally arranged in a sealed bag filled with nitrogen, the time and the labor are saved, and the use requirement is convenient to meet;

the box door 7 is a vertical hinged door, the box door 7 is hinged at the opening position of the box body 1, the box door 7 is a sealing door, and a glass sealing door is further arranged in the box door 7.

Further, still include gyro wheel 3, gyro wheel 3 installs in the bottom of box 1, and gyro wheel 3 is convenient for remove box 1.

Further, still include attacker 5, attacker 5 installs in the end outside that opens and shuts of vertical hinged door 7, and attacker 5 is ABS attacker, and attacker 5 conveniently carries out the switching operation to chamber door 7.

Furthermore, a magnetic sealing gasket 8 is arranged at the joint of the box door 7 and the opening of the box body 1, so that the sealing performance inside the box body 1 when the box door 7 is closed is ensured.

Further, control module 6 still is connected with the display screen, shows the nitrogen gas concentration in the box 1 on the display screen, monitors the nitrogen gas concentration in the box 1 through nitrogen gas concentration sensor 4, and the information transmission of monitoring is for control module 6, and control module 6 carries out analysis processes and shows through the display screen to information, has guaranteed the rationality of design.

Further, still include active carbon filter screen 9, active carbon filter screen 9 sets up in the breather pipe, and active carbon filter screen 9 is arranged in filtering the dust in the air, has guaranteed the dustless environment of box 1 inside.

Through nitrogen gas concentration sensor 4 to the nitrogen gas concentration in the box 1 monitor, the information transmission of monitoring is for control module 6, and control module 6 carries out analysis processes and shows through the display screen to the information.

The activated carbon filter screen 9 is used for filtering dust in the air.

It is noted that the control module 6 disclosed in the above embodiment is an MCS-51 series single chip microcomputer, and the nitrogen concentration sensor 4 is a model LY 800-N2.

The above only is the embodiment of the present invention, not limiting the scope of the present invention, all the equivalent structures or equivalent processes of the present invention are used in the specification and the attached drawings, or directly or indirectly applied to other related technical fields, and the same principle is included in the protection scope of the present invention.

Claims

1. A cell culture box capable of carrying out an oxygen deficiency experiment comprises a box body (1), a box door (7) and a plurality of layers of shelving plates (2) arranged in the box body (1), wherein a control module (6) is arranged on the front side of the top of the box body (1);

the device is characterized in that a scavenging pipe with a blower and a one-way air outlet are arranged on the box body (1), the scavenging pipe is connected with a three-way valve (10), a first pipeline interface of the three-way valve (10) is connected with the scavenging pipe, a second pipeline interface and a third pipeline interface of the three-way valve (10) are respectively connected with a nitrogen source and a clean air source, the air source communicated with the scavenging pipe is controlled through the adjustment of the three-way valve (10), a nitrogen concentration sensor (4) is arranged on the inner wall of the box body (1), and the nitrogen concentration sensor (4) is electrically connected with the control module (6) so as to monitor the change of the nitrogen concentration in the box body (1);

the box door (7) is a vertical hinged door, the box door (7) is hinged to the opening position of the box body (1), and the box door (7) is a sealing door.

2. A cell culture chamber capable of conducting hypoxia experiments according to claim 1, wherein: the box body is characterized by further comprising rollers (3), wherein the rollers (3) are installed at the bottom of the box body (1).

3. A cell culture chamber capable of conducting hypoxia experiments according to claim 1, wherein: still include attacker (5), attacker (5) are installed in the end outside that opens and shuts of chamber door (7), and attacker (5) are ABS attacker.

4. A cell culture chamber capable of conducting hypoxia experiments according to claim 1, wherein: and a magnetic sealing gasket (8) is arranged at the joint of the box door (7) and the opening of the box body (1).

5. A cell culture chamber capable of conducting hypoxia experiments according to claim 1, wherein: the control module (6) is also connected with a display screen, and the nitrogen concentration in the box body (1) is displayed on the display screen.

6. A cell culture chamber capable of conducting hypoxia experiments according to claim 1, wherein: still include active carbon filter screen (9), active carbon filter screen (9) set up in the breather pipe.