CN220376706U - Cell culture safety box with active gas exchange function - Google Patents
Cell culture safety box with active gas exchange function Download PDFInfo
- Publication number
- CN220376706U CN220376706U CN202321361334.2U CN202321361334U CN220376706U CN 220376706 U CN220376706 U CN 220376706U CN 202321361334 U CN202321361334 U CN 202321361334U CN 220376706 U CN220376706 U CN 220376706U
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- safety box
- air
- cell culture
- cover plate
- shell
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- 238000004113 cell culture Methods 0.000 title claims abstract description 22
- 230000001681 protective effect Effects 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 230000001105 regulatory effect Effects 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 4
- 238000000034 method Methods 0.000 abstract description 10
- 230000007547 defect Effects 0.000 abstract description 2
- 238000012546 transfer Methods 0.000 description 8
- 238000012258 culturing Methods 0.000 description 6
- 230000010261 cell growth Effects 0.000 description 5
- 238000002156 mixing Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000003292 glue Substances 0.000 description 2
- 230000012010 growth Effects 0.000 description 2
- 206010021143 Hypoxia Diseases 0.000 description 1
- 238000007792 addition Methods 0.000 description 1
- XAGFODPZIPBFFR-UHFFFAOYSA-N aluminium Chemical compound [Al] XAGFODPZIPBFFR-UHFFFAOYSA-N 0.000 description 1
- 229910052782 aluminium Inorganic materials 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 238000002844 melting Methods 0.000 description 1
- 230000008018 melting Effects 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000009423 ventilation Methods 0.000 description 1
Abstract
The utility model discloses a cell culture safety box with active gas exchange, which comprises a safety box body and an air source switching instrument, wherein the safety box body is connected with the air source switching instrument through a connector joint. The utility model solves the defects that the cell culture container is easy to pollute in the transferring and observing process, the safety box is required to be uniformly mixed in advance for supplying air, the safety box has larger volume, and the like. The cell culture container can be provided with a stable culture environment, and gas is directly input into the safety box from the incubator, so that the environment in the safety box can be rapidly consistent with that in the incubator. And the safety box is small in size, convenient to assemble and disassemble, convenient to transport and carry, transparent in cover plate and convenient to observe.
Description
Technical Field
The utility model relates to the field of biomedical equipment, in particular to a cell culture safety box with active gas exchange.
Background
In recent years, the field of biological medicine is continuously developed, cell research is continuously in depth, the application scale is continuously expanded, and the market scale of cell culture is continuously expanded. The cell culturing process necessarily involves a cell culturing box, and the specific environment inside the culturing box is the guarantee of healthy growth of cells, but in daily operation, an experimenter needs to open a culturing box door at regular time and take out a culturing container for culturing state observation. For conventional incubators, whenever the incubator door is opened, CO 2 、O 2 The temperature and humidity will change dramatically, after which the incubator takes several minutes to readjust to the set point level. When cells are subjected to changes in environmental conditions from outside the culture environment for a long period of time, cell growth may be affected. Moreover, when a plurality of culture individuals are accommodated in the incubator, frequent opening and closing of the culture door may also risk cross contamination of the culture and the indoor air particles. Therefore, a technology is needed to solve the above-mentioned problems and avoid the pollution risk in the cell culture process.
Publication (bulletin) number: CN113999760a, publication (date): 2021-09-22 relates to a cell aseptic transfer container and a cell aseptic transfer method, wherein the cell aseptic transfer container is used for preparing cells in a class A environment in the whole process from preparation to culture, the cell aseptic transfer container is provided with a sealed container, two ends of the sealed container are provided with ventilation openings, and the openings are provided with filter membranes for filtering microorganisms; the opening is also provided with a plugging object for sealing the sealed container. However, the proposal can only ensure that the cell culture container is in a class A environment, and the cell state needs to be taken out after the subsequent observation, thus being easy to be polluted again.
Publication (bulletin) number: CN218058989U, publication (date): 2022-12-16 relates to a portable cell hypoxia culture device, which comprises a cell culture box and further comprises a gas mixing device, wherein the gas mixing device is connected with the cell culture box through a mixed gas output pipe, a gas pump is arranged on the mixed gas output pipe, and the mixed gas is input into the culture box after being mixed, so that the gas environment is more uniform and stable. However, the gas mixing process is complicated, and an additional gas mixing device is required to be carried, so that the volume of the culture box is reduced but the carrying convenience is not achieved.
Disclosure of Invention
The utility model aims to solve the defects in the prior art, and provides a device (a cell culture safety box) which is convenient to observe, can directly supply air, has smaller box body volume and is more convenient to carry and transport, and can accommodate various cell culture containers in the market, including T-shaped bottles, petri dishes, porous plates and the like.
In order to achieve the above purpose, the present utility model provides the following solutions: the utility model provides a cell culture safety box of gaseous initiative exchange, includes the whole and air supply switching appearance of safety box, and the whole safety box shell that includes of safety box is equipped with safety box air inlet and safety box gas outlet, and the safety box gas outlet external has the connector to connect, and the connector connects and is connected with the air supply switching appearance through the air feed interface.
Preferably, the air source switch is connectable to at least one connector fitting, including but not limited to a quick connector fitting or the like.
Preferably, the bacteria filter is installed at the air inlet port and the air outlet port of the safety box, and the bacteria filter forms comprise, but are not limited to, bacteria filters with various material specifications and the like.
Preferably, the air source switching instrument shell comprises an air supply interface and an air vent, the air supply interface is connected with a channel 1 switching valve and a channel 2 switching valve, the switching valves are controlled by a total air source switching valve, an air inlet air source connector is arranged on the opposite side of the air supply interface, and the number of the air supply interface and the number of the channel switching valves are more than or equal to two.
Preferably, the gas source switcher comprises a switcher control board and a gas flow regulating module, wherein the switcher control board comprises, but is not limited to, a solenoid valve and the like, and the gas flow regulating module comprises, but is not limited to, a gas pump, a blower and the like.
Preferably, the safety box shell is provided with a top cover plate and a bottom cover plate, both sides of the safety box shell are provided with fence-shaped semi-surrounding protective shells, the upper ends of the protective shells are 1-2cm higher than the top cover plate, materials of the safety box shell comprise but are not limited to PC, aluminum, ABS and the like, and materials of the top cover plate and the bottom cover plate comprise but are not limited to transparent glass and the like.
Preferably, the top cover plate is connected to the safety box housing by a latch, the connection including, but not limited to, a seal ring.
Preferably, the lock catch is arranged at the opposite side of the connector joint, the lock catch is connected with the top cover plate through the lock catch piece, and the top cover plate is connected with the safety box shell through the cover plate fastener.
Preferably, the fastening means of the lock catch and the cover plate fastener and the safety box shell comprise but are not limited to hot melting and glue adhesion, and the fastening means of the lock catch and the cover plate fastener and the top cover plate comprise but are not limited to fastening means, glue adhesion and the like.
The beneficial effects of the utility model are as follows:
(1) Compared with the prior art, the air source switching instrument can directly collect air from the incubator, can enable the internal environment of the safety box to be rapidly consistent with the environment in the incubator, avoids the complex process that an external air source needs to be uniformly mixed and refilled, and the valve port is automatically closed when the connector joint is disconnected from the air supply interface, so that the influence of external air on the internal environment of the safety box when the incubator is opened is avoided.
(2) The safety box air inlet and the safety box air outlet of the safety box shell are both provided with bacteria filters, so that the gas entering from the incubator can be ensured to be sterile, and the gas exhausted from the safety box can be ensured to be sterile.
(3) The safety box is convenient to connect with the air source switching instrument, is simple to detach, does not need to carry additional air sources and assembly parts, is convenient to transport and assemble and recover, and is more convenient in flow.
(4) The top cover plate and the bottom cover plate adopt a high-permeability structural design, so that the cell growth condition can be continuously observed without taking out the culture dish and the like after the cell growth device is matched with the cell on-line growth device.
Drawings
Fig. 1 is a schematic diagram of the operation of the present utility model.
Fig. 2 is a top structural view of the present utility model.
Fig. 3 is a bottom structural view of the present utility model.
Fig. 4 is an internal structural view of the present utility model.
Fig. 5 is a top view of the structure of the present utility model.
Fig. 6 is a rear block diagram of the present utility model.
FIG. 7 is a block diagram of an air source switching apparatus of the present utility model.
The reference numerals in the figures illustrate: 1-safety box whole, 2-air source switching instrument, 2.1-air supply interface, 2.2-bleeder vent, 2.31-channel 1 switch valve, 2.32-channel 2 switch valve, 2.4-total air source switch valve, 2.5-air inlet air source joint, 2.6-switching instrument control panel, 2.7-air flow regulating module, 3-safety box shell, 4-top cover plate, 5-cover plate fastener, 6-fastener, 7-fastener, 8-bottom cover plate, 9-bacteria filter, 9.1-safety box air inlet, 9.2-safety box air outlet, 10-connector joint.
Detailed Description
Specific embodiments of the utility model will be described in detail below, with the understanding that the embodiments described herein are merely some, but not all embodiments of the utility model. All other embodiments, based on the embodiments described below, which a person of ordinary skill in the art would obtain without making any inventive effort, are within the scope of the utility model.
The utility model provides a technical scheme that:
the cell culture safety box with active gas exchange includes one safety box body 1 and one gas source switcher 2 connected via connector joint 10.
The safety box comprises a safety box shell 3, the safety box shell 3 is provided with a safety box air inlet 9.1 and a safety box air outlet 9.2, and the bacteria filter 9 is arranged at the port of the air inlet and outlet so that both input gas and exhaust gas can be filtered.
The safety box air outlet 9.2 is externally connected with a connector joint 10, the connector joint 10 is connected with the air source switching instrument 2 through an air supply interface 2.1, and the air source switching instrument 2 can be connected with at least one connector joint 10 to ensure that a plurality of safety boxes can be supplied with air simultaneously.
The air source switching instrument 2 shell comprises an air supply interface 2.1 and an air vent 2.2, the air supply interface 2.1 is connected with a channel 1 switching valve 2.31 and a channel 2 switching valve 2.32, the switching valves are controlled by a total air source switching valve 2.4, an air inlet air source connector 2.5 is arranged on the opposite side of the air supply interface 2.1 of the air source switching instrument 2 shell, the air source switching instrument 2 further comprises a switching instrument control board 2.6 and an air flow regulating module 2.7, and the process of inputting air from the incubator into the safety box is realized through control of the switching valve and control of air flow.
The safety box housing 3 is provided with a top cover plate 4 and a bottom cover plate 8 which are made of high-permeability materials, so that the safety box housing is convenient to cooperate with online observation equipment to conduct continuous observation.
The side of the safety box shell 3 is provided with a fence-shaped semi-surrounding protective shell, and the upper end of the protective shell is 1-2cm higher than the top cover plate, so that the protective shell plays a role in protecting the top cover plate 4.
The top cover plate 4 is connected with the safety box shell 3 through a lock catch 7, so that the safety box can be conveniently opened and closed, and a sealing ring is arranged at the joint.
The lock catch 7 is fixed on the side face of the safety box shell 3, the top cover plate 4 is connected with the lock catch 7 through the lock catch piece 6, the sealing effect is achieved, and the other end of the top cover plate 4 is fixed on the top end of the safety box shell 3 through the cover plate fastener 6.
When in use, the culture container moving and taking operation is carried out according to the following steps:
the culture container is transferred to the safety box, the safety box is sealed again through the lock catch 7, the air inlet source connector 2.5 is connected with the culture box, the air inlet connector 2.1 is connected with the connector 10, the valve port is ejected, and the control switching instrument control board 2.6 and the air flow regulating module 2.7 supply air to the safety box according to requirements.
After the incubator works for a period of time, when the incubator is full of gas, the connection is disconnected, the valve port is automatically closed, and the incubator returns to the closed state again.
After the transfer of the culture container is completed, the transfer can be carried out, or the cell growth condition can be continuously observed by being matched with cell on-line growth equipment.
Compared with the prior art, the utility model has the beneficial effects that the pollution problem possibly existing in the processes of cell culture and cell container transfer is solved; the gas in the incubator is directly input into the safety box, so that a complex process of uniformly mixing and then inputting is avoided, and a transfer process is reduced; the safety box is convenient to observe, smaller in size and convenient to carry.
The foregoing has shown and described the basic features, principles, and advantages of the utility model. It should be noted that the present utility model is not limited to the above-described embodiments, but is only limited to a few embodiments, and modifications and additions can be made thereto without departing from the spirit and scope of the utility model.
Claims (6)
1. The utility model provides a cell culture safety box of gas initiative exchange, its characterized in that, including safety box whole (1) and air supply switching appearance (2), safety box whole (1) include safety box shell (3), and safety box shell (3) are equipped with safety box air inlet (9.1) and safety box gas outlet (9.2), safety box gas outlet (9.2) external connector joint (10) that has, connector joint (10) are connected with air supply switching appearance (2) through air feed interface (2.1).
2. The cell culture safety box with active gas exchange according to claim 1, wherein the bacteria filter (9) is arranged at the port of the air inlet (9.1) and the port of the air outlet (9.2) of the safety box.
3. The cell culture safety box according to claim 1 or 2, wherein the air source switcher (2) shell comprises an air supply interface (2.1) and an air vent (2.2), the air supply interface (2.1) is connected with a channel 1 switch valve (2.31) and a channel 2 switch valve (2.32), the switch valves are controlled by a total air source switch valve (2.4), an air supply connector (2.5) is arranged on the opposite side of the air source switcher (2.1) shell, and the air source switcher (2) further comprises a switcher control board (2.6) and an air flow regulating module (2.7).
4. A cell culture safety box according to claim 1 or 2, characterized in that the safety box shell (3) is provided with a top cover plate (4) and a bottom cover plate (8), both sides of the safety box shell (3) are provided with fence-shaped semi-surrounding protective shells, and the upper ends of the protective shells exceed the top cover plate (4).
5. The cell culture safety box with active gas exchange according to claim 4, wherein the top cover plate (4) is connected with the safety box shell (3) through a lock catch (7), and a sealing ring is arranged at the connection part.
6. The cell culture safety box according to claim 5, wherein the lock catch (7) is arranged at the opposite side of the connector joint (10), the lock catch (7) is connected with the top cover plate (4) through the lock catch (6), and the top cover plate (4) is connected with the safety box shell (3) through the lock catch (6).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321361334.2U CN220376706U (en) | 2023-05-31 | 2023-05-31 | Cell culture safety box with active gas exchange function |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202321361334.2U CN220376706U (en) | 2023-05-31 | 2023-05-31 | Cell culture safety box with active gas exchange function |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN220376706U true CN220376706U (en) | 2024-01-23 |
Family
ID=89564541
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202321361334.2U Active CN220376706U (en) | 2023-05-31 | 2023-05-31 | Cell culture safety box with active gas exchange function |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN220376706U (en) |
-
2023
- 2023-05-31 CN CN202321361334.2U patent/CN220376706U/en active Active
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