CN115011473A - Microfluidic perfusion culture integrated chip card box for space environment and preparation method thereof - Google Patents

Microfluidic perfusion culture integrated chip card box for space environment and preparation method thereof Download PDF

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Publication number
CN115011473A
CN115011473A CN202210808260.6A CN202210808260A CN115011473A CN 115011473 A CN115011473 A CN 115011473A CN 202210808260 A CN202210808260 A CN 202210808260A CN 115011473 A CN115011473 A CN 115011473A
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card box
middle frame
culture
perfusion culture
integrated chip
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邓玉林
徐建栋
张莉明
任培
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Beijing Ligong Genshu Technology Co ltd
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Beijing Ligong Genshu Technology Co ltd
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Priority to CN202210808260.6A priority Critical patent/CN115011473A/en
Publication of CN115011473A publication Critical patent/CN115011473A/en
Priority to PCT/CN2023/093003 priority patent/WO2024012029A1/en
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/14—Bags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/16—Microfluidic devices; Capillary tubes
    • C—CHEMISTRY; METALLURGY
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    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/38—Caps; Covers; Plugs; Pouring means
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/54—Constructional details, e.g. recesses, hinges hand portable
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/10—Perfusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/14—Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus with filters, sieves or membranes
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M37/00—Means for sterilizing, maintaining sterile conditions or avoiding chemical or biological contamination
    • C12M37/04—Seals
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    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
    • C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes

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Abstract

The invention provides a microfluidic perfusion culture integrated chip card box for a space environment, which comprises a card box frame structural body, a microfluidic perfusion culture module and an airtight electric connector, wherein the card box frame structural body is a full-sealing structure formed by a middle frame plate, an upper cover plate and a lower cover plate, the microfluidic perfusion culture module is sealed in the card box frame structural body, and the card box frame structural body is connected with external control equipment through the airtight electric connector. The microfluidic perfusion culture integrated chip card box is totally closed, and microorganisms in the integrated chip card box are isolated from outside operators, so that the integrated chip card box is suitable for being used in a space environment.

Description

Microfluidic perfusion culture integrated chip card box for space environment and preparation method thereof
Technical Field
The invention belongs to the field of biomedical detection, and particularly relates to a microfluidic perfusion culture integrated chip card box for a space environment and a preparation method thereof.
Background
In the direction of space life science and biotechnology, long-term microgravity, regular magnetic fields, rapid alternation between day and night, special radiation and other environmental conditions provided by a space station are utilized to promote understanding and understanding of the nature of life phenomena and explore scientific laws, researches on induction of a life body to gravity change, damage under space radiation, origin of life, evolution, development, reproduction and the like are carried out, biotechnology development and application researches on the utilization of space conditions are carried out, development of modern life science and biotechnology is promoted, and human health and social progress are served.
The requirement on the sealing performance of a cell culture device is higher in a space environment, a perfusion culture chip and perfusion equipment in the prior art need to be connected through a liquid pipeline, and the chip is inconvenient to replace and reconnect the pipeline in the space environment; the chip is externally connected with a perfusion pipeline, and if the chip is exposed in the equipment, the risk of liquid leakage exists, the normal operation of the equipment is influenced, and even the space environment is polluted; if the chip and the perfusion device are integrated, the mechanical and control electric devices cannot be reused, and load waste is caused.
Disclosure of Invention
In order to overcome the problems in the prior art, the invention provides a replaceable microfluidic perfusion culture integrated chip card box which is suitable for space environment. The function is that the required culture medium of microbial cultivation, culture chamber and parts such as liquid flow path and control valve are integrated to inside the micro-fluidic chip card box, provide totally enclosed microbial cultivation and detection environment, reduce the artifical change operation degree of difficulty of microbial cultivation chip, simultaneously, avoided the biological sample at the change in-process to reveal the risk.
The invention provides the following technical scheme:
the microfluidic perfusion culture integrated chip card box for the space environment comprises a card box frame structural body, a microfluidic perfusion culture module and an airtight electric connector, wherein the card box frame structural body is of a full-sealing structure formed by a middle frame plate, an upper cover plate and a lower cover plate, the microfluidic perfusion culture module is sealed inside the card box frame structural body, and the card box frame structural body is connected with external control equipment through the airtight electric connector.
Furthermore, the microfluidic perfusion culture module and the airtight electric connector are arranged in a middle frame plate, two sides of the middle frame plate corresponding to the upper cover plate and the lower cover plate are provided with annular sealing grooves, sealing rings are arranged in the sealing grooves, and the upper cover plate and the lower cover plate are fixed with the middle frame plate through bolts.
Furthermore, the microfluidic perfusion culture module comprises a liquid storage unit, an injection pump unit, a liquid separation control unit and a culture detection chamber unit.
Further, cultivate and detect the chamber unit and fix on dividing the liquid control unit, cultivate and detect the chamber unit and constitute sealed cultivation chamber by upper cover plate and middle orifice plate, be equipped with heating plate and temperature sensor between upper cover plate and the middle orifice plate, be equipped with microorganism filtration membrane and seal ring between middle orifice plate and the bottom divides liquid control unit to it links to each other with the flow path to cultivate the chamber through the filtration membrane.
Further, the stock solution bag of stock solution unit, waste liquid bag pass through the bolt fastening in middle deckle board, and stock solution mouth and waste liquid mouth are connected to liquid inlet channel, the liquid outlet channel who divides liquid control unit respectively on, and the syringe pump unit passes through luer articulate on dividing liquid control unit, with stock solution bag liquid way intercommunication, the syringe pump body passes the lateral wall of middle deckle board, and the inboard position that corresponds with the syringe pump body of middle deckle board is equipped with the seal groove, is equipped with O type circle in the seal groove.
Furthermore, a hollow sealing screw is arranged on the outer side of the middle frame plate, the injection pump body extending out of the middle frame plate is fixed, and a detachable limiting block is arranged on the outer side of the middle frame plate and used for limiting the push rod of the injection pump to move transversely when being stored.
Furthermore, the liquid separation control unit is provided with an electromagnetic valve for controlling the opening and closing of the liquid inlet channel and the liquid outlet channel.
Furthermore, the length of the card box frame structure body is 190mm, the width is 80-100mm, the thickness is 20-25mm, the volume of the culture detection chamber is 200-300 uL, the volume of the liquid storage bag and the waste liquid bag is 5-10 mL, and the volume of the injection pump is 200-2000 uL.
Furthermore, a handle is arranged on the side surface of the card box frame structure body.
Furthermore, an airtight electric connector is fixed on the middle frame plate, the airtight electric connector is respectively connected with the heating plate, the temperature sensor and the electromagnetic valve, and the outside of the airtight electric connector is inserted into the control equipment.
A preparation method of a microfluidic perfusion culture integrated chip card box for a space environment comprises the following steps:
step one set of assembly of microfluidic perfusion culture module of claim 1, connecting the liquid storage unit, the injection pump unit, the culture detection chamber unit to the liquid separation control unit, pre-installing microbial dry powder, culture medium and the like in each functional unit according to requirements, wherein the leads of the electromagnetic valve, the heating film and the temperature sensor are connected with the airtight electric connector;
step two, fixedly connecting the microfluidic perfusion culture module on the middle frame plate, wherein the fixation comprises a liquid storage bag, a waste liquid bag and a pipeline thereof;
step three, the injection pump penetrates through the middle frame plate, the O ring is sleeved outside the injection pump body, the injection pump is locked and fixed on the Ruhr connector, the O ring is fed into the sealing groove of the middle frame plate, the hollow sealing screw is sleeved and fixed outside the injection pump body, and the limiting block is clamped and connected with the push rod;
and fourthly, installing sealing rings in the annular sealing grooves on the inner side and the outer side of the middle frame plate, and connecting the upper cover plate and the lower cover plate with the periphery of the middle frame plate by using bolts.
By adopting the technical scheme, the invention has the following beneficial effects:
(1) the microfluidic perfusion culture integrated chip card box is totally closed, and microorganisms in the integrated chip card box are isolated from outside operators, so that the integrated chip card box is suitable for being used in a space environment.
(2) The full integration, culture medium, perfusion pump, growth detection chamber, constant temperature part and waste liquid that the microorganism growth needs are deposited and are all designed into unit subassembly, integrate in the module.
(3) The module is used for separately embedding the sterilization culture medium and the freeze-dried powder and storing the sterilized culture medium and the freeze-dried powder in a cold storage way. The operation is easy, the modularized design is adopted, and the operation is matched with external control equipment. When the card box is used, an operator only needs to take the card box out of the package, insert the card box into external equipment according to requirements and start a program.
Drawings
FIG. 1 is a schematic structural diagram of a microfluidic perfusion culture integrated cartridge according to the present invention;
FIG. 2 is a schematic diagram of the microfluidic perfusion culture module according to the present invention;
FIG. 3 is a schematic diagram of the side view of the culture detection chamber of the present invention.
Wherein: the kit comprises a card box frame structure body 1, a hermetic electric connector 2, a microfluidic perfusion culture module 3, a culture detection chamber unit 4, an upper cover plate 41, a heating sheet 42, a filtering membrane 43, a sealing ring 44, a liquid storage bag 5, a waste liquid bag 6, an injection pump body 7, a hollow sealing screw 8, a limiting block 9, an electromagnetic valve 10 and a handle 11.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention will be described in further detail with reference to the accompanying drawings and embodiments. It should be understood that the block diagrams and specific examples are set forth only for the purpose of illustrating the invention and are not to be construed as limiting the invention.
Example 1
As shown in fig. 1 and 2, the invention provides a microfluidic perfusion culture integrated chip card box for a space environment, which comprises a card box frame structure body 1, a microfluidic perfusion culture module 3 and an airtight electric connector 3, wherein the card box frame structure body is a full-sealing structure formed by a middle frame plate, an upper cover plate and a lower cover plate, the microfluidic perfusion culture module is sealed in the card box frame structure body, and the card box frame structure body is connected with external control equipment through the airtight electric connector.
The microfluidic perfusion culture module and the airtight electric connector are arranged in the middle frame plate, two sides of the middle frame plate corresponding to the upper cover plate and the lower cover plate are provided with annular sealing grooves, sealing rings are arranged in the sealing grooves, and the upper cover plate and the lower cover plate are fixed with the middle frame plate through bolts. The microfluidic perfusion culture module is used for culturing and detecting biological samples, and the airtight electric connector is connected with an external power supply and a controller to control electric elements in the integrated chip card box. Through a set perfusion culture program, the perfusion culture card box is controlled to achieve the functions of constant culture temperature, recovering the microorganisms to be cultured, providing culture medium perfusion with fixed time and fixed quantity, detecting OD values and fluorescence value data of the microorganisms and the like.
The length of the card box frame structure body is 150-190mm, the width is 80-100mm, and the thickness is 20-25 mm. Preferably, the cartridge frame structure has a length of 155mm, a width of 85mm and a thickness of 24 mm. The side of the card box frame structure body is provided with a handle. The operator only contacts the outer surface of the perfusion culture card box, so that the failure of microorganism culture caused by pollution introduced in the operation of the operator is avoided; simultaneously, the microorganism of card box inside also can not reveal in the environment that operating personnel belongs to, avoids causing the pollution to the environment.
Example 2
As shown in fig. 3, the microfluidic perfusion culture module includes a liquid storage unit, an injection pump unit, a liquid separation control unit, and a culture detection chamber unit. The culture detection chamber unit 4 is fixed on the liquid separation control unit, the culture detection chamber unit comprises a sealed culture chamber formed by an upper cover plate 41 and a middle orifice plate, a heating plate 42 and a temperature sensor are arranged between the upper cover plate and the middle orifice plate, a microorganism filtering membrane 43 and a sealing washer 44 are arranged between the middle orifice plate and the bottom liquid separation control unit, and the culture chamber is connected with the flow path through the filtering membrane. The volume of the culture detection chamber can be 200-300 uL, and 300uL is preferred.
Liquid storage bag 5, the waste liquid bag 6 of stock solution unit pass through the bolt fastening in middle deckle board, and stock solution mouth and waste liquid mouth are connected to liquid inlet channel, the liquid outlet channel of separating the liquid control unit respectively on, and stock solution bag, waste liquid bag volume can be 5 ~ 10mL, preferred 6 mL.
The injection pump body 7 is arranged in the middle frame plate, is fixed on the liquid separation control unit through a luer connector and is communicated with the liquid path of the liquid storage bag, and the volume of the injection pump can be 1-2mL, preferably 1 mL. The injection pump body penetrates through the side wall of the middle frame plate, a sealing groove is formed in the position, corresponding to the injection pump body, of the inner side of the middle frame plate, and an O-shaped ring is arranged in the sealing groove.
The outer side of the middle frame plate is provided with a hollow sealing screw 8 for fixing the injection pump body extending out of the middle frame plate, and the outer side of the middle frame plate is provided with a detachable limiting block 9 for limiting the push rod of the injection pump to move transversely when being stored.
The liquid separation control unit is provided with an electromagnetic valve for controlling the opening and closing of the liquid inlet channel and the liquid outlet channel. The number of the electromagnetic valves is suitable for the number of the culture chambers. The airtight electric connector is fixed on the middle frame plate, the airtight electric connector is respectively connected with the heating sheet, the temperature sensor and the electromagnetic valve, and the outside of the airtight electric connector is plugged with the control equipment. The control equipment provides on-off control of two paths of electromagnetic valves, real-time temperature reading of a temperature sensor, power supply of a heating film and the like; meanwhile, the external control equipment also provides the push-pull power of the injection pump.
The resuscitation and culture control process of the microfluidic perfusion culture integrated chip card box comprises the following steps:
1) inserting the chip card box into the device, and starting a control program;
2) firstly, controlling a heating film to start heating, simultaneously monitoring the temperature of a culture detection chamber unit by a temperature sensor, and feeding back and adjusting the heating film to ensure that the temperature of a chamber reaches and is constant at 37 ℃;
3) and (3) microorganism recovery stage:
a. the device pulls a push rod of an injection pump of the chip card box to suck 800uL of culture medium in the liquid storage bag;
b. opening the electromagnetic valve 1, connecting the flow path between the injection pump and the culture chamber 1, and pushing the injection pump to perfuse 400uL of culture medium to the culture chamber 1;
c. keeping the valve 1 in an open state, opening the valve 2, switching the flow path of the injection pump to the culture chamber 2, continuously pushing the injection pump to perfuse 400uL of culture medium, and completing the liquid perfusion of the two chambers;
d. and (4) closing the valve 1 and the valve 2, and keeping the temperature at 37 ℃ for 1h to completely recover the microbial dry powder.
4) Continuous perfusion culture and detection stage:
a. according to the research requirement, the microorganisms are detected by the transparent window of the culture detection chamber unit according to the OD value or the fluorescence value;
b. the device pulls a push rod of an injection pump of the chip card box to suck 800uL of culture medium in the liquid storage bag;
c. opening the electromagnetic valve 1, connecting the flow path between the injection pump and the culture chamber 1, and pushing the injection pump to perfuse 15uL of culture medium to the culture chamber 1;
d. keeping the valve 1 in an open state, opening the valve 2, switching the flow path of the injection pump to the culture chamber 2, continuously pushing the injection pump to perfuse 15uL of culture medium, and completing the liquid perfusion of the two chambers;
e. closing the valve 1 and the valve 2, keeping static culture for 10min, and then completing intermittent continuous perfusion after repeating the operations of c and d;
f. detection can be completed before each perfusion or continuous detection can be performed according to requirements;
g. the whole perfusion culture process can last for 8-12 hours according to experiment needs, and the step b can be repeated to continue sucking from the liquid storage bag when the liquid amount of the injection pump in the midway is not enough.
5) End and close phases:
a. after the culture is finished, the equipment automatically resets the injection pump to a zero position, stops heating and closes the electromagnetic valve;
b. and taking out the perfusion culture integrated chip card box, packaging and then discarding.
Example 3
The invention provides a preparation method of a microfluidic perfusion culture integrated chip card box for a space environment, which comprises the following steps:
step one set of assembly of microfluidic perfusion culture module of claim 1, connecting the liquid storage unit, the injection pump unit, the culture detection chamber unit to the liquid separation control unit, pre-installing microbial dry powder, culture medium and the like in each functional unit according to requirements, wherein the leads of the electromagnetic valve, the heating film and the temperature sensor are connected with the airtight electric connector;
step two, fixedly connecting the microfluidic perfusion culture module on the middle frame plate, wherein the fixation comprises a liquid storage bag, a waste liquid bag and a pipeline thereof;
step three, the injection pump penetrates through the middle frame plate, the O ring is sleeved outside the injection pump body, the injection pump is locked and fixed on the Ruhr connector, the O ring is fed into the sealing groove of the middle frame plate, the hollow sealing screw is sleeved and fixed outside the injection pump body, and the limiting block is clamped and connected with the push rod;
and fourthly, installing sealing rings in the annular sealing grooves on the inner side and the outer side of the middle frame plate, and connecting the upper cover plate and the lower cover plate with the periphery of the middle frame plate by using bolts.
Through experimental test, the whole ability that provides of card box of this application is whole sealed, when card box internal pressure reached 1.2 atmospheric pressure, still can guarantee that the card box content can not reveal. It can be ensured that the cartridge does not risk leakage in a vacuum environment or in extreme cases when the cartridge exterior is completely depressurized.
The above-mentioned embodiments only express the embodiments of the present invention, and the description thereof is more specific and detailed, but not construed as limiting the scope of the present invention. It should be noted that various changes and modifications can be made by those skilled in the art without departing from the spirit of the invention, and these changes and modifications are all within the scope of the invention. Therefore, the protection scope of the present patent shall be subject to the appended claims.

Claims (11)

1. The utility model provides a space environment cultivates integrated chip card box with micro-fluidic perfusion, includes card box frame structure, micro-fluidic perfusion cultivation module and airtight type electric connector, its characterized in that, card box frame structure forms full seal structure by middle deckle board, upper and lower apron, micro-fluidic perfusion cultivation module seals inside card box frame structure, and card box frame structure passes through airtight type electric connector and is connected with external control equipment.
2. The integrated cartridge of microfluidic perfusion culture according to claim 1, wherein the microfluidic perfusion culture module and the airtight electric connector are disposed in a middle frame plate, two sides of the middle frame plate corresponding to the upper and lower cover plates are provided with annular sealing grooves, sealing rings are disposed in the sealing grooves, and the upper and lower cover plates are fixed to the middle frame plate by bolts.
3. The microfluidic perfusion culture integrated chip cartridge of claim 2, wherein the microfluidic perfusion culture module comprises a liquid storage unit, an injection pump unit, a liquid separation control unit and a culture detection chamber unit.
4. The microfluidic perfusion culture integrated chip cartridge according to claim 3, wherein the culture detection chamber unit is fixed on the liquid separation control unit, the culture detection chamber unit comprises a sealed culture chamber consisting of an upper cover plate and a middle orifice plate, a heating plate and a temperature sensor are arranged between the upper cover plate and the middle orifice plate, a microorganism filtering membrane and a sealing gasket are arranged between the middle orifice plate and the bottom liquid separation control unit, and the culture chamber is connected with the flow path through the filtering membrane.
5. The microfluidic perfusion culture integrated chip cartridge of claim 3, wherein the liquid storage bag and the waste liquid bag of the liquid storage unit are fixed in the middle frame plate through bolts, the liquid storage port and the waste liquid port are respectively connected to the liquid inlet channel and the liquid outlet channel of the liquid separation control unit, the injection pump unit is connected to the liquid separation control unit through a luer connector and is communicated with the liquid path of the liquid storage bag, the injection pump body penetrates through the side wall of the middle frame plate, a sealing groove is arranged on the inner side of the middle frame plate and corresponds to the injection pump body, and an O-shaped ring is arranged in the sealing groove.
6. The microfluidic perfusion culture integrated chip cartridge of claim 5, wherein a hollow sealing screw is disposed at an outer side of the middle frame plate to fix the syringe pump body extending out of the middle frame plate, and a detachable stopper is disposed at an outer side of the middle frame plate to limit a push rod of the syringe pump from moving laterally when stored.
7. The microfluidic perfusion culture integrated chip card box of claim 3, wherein the liquid separation control unit is provided with an electromagnetic valve for controlling the opening and closing of the liquid inlet channel and the liquid outlet channel.
8. The microfluidic perfusion culture integrated chip cartridge as claimed in claim 2, wherein the cartridge frame structure has a length of 150-190mm, a width of 80-100mm, a thickness of 20-25mm, a culture detection chamber volume of 200-300 uL, a reservoir bag and a waste liquid bag volume of 5-10 mL, and an injection pump volume of 200-2000 uL.
9. The microfluidic perfusion culture integrated chip cartridge of claim 2, wherein a handle is provided at a side of the cartridge frame structure.
10. The microfluidic perfusion culture integrated chip cartridge according to claim 4 or 7, wherein an airtight electric connector is fixed on the middle frame plate, the airtight electric connector is respectively connected with the heating plate, the temperature sensor and the electromagnetic valve, and the external part of the airtight electric connector is plugged with the control device.
11. A preparation method of the microfluidic perfusion culture integrated chip card box for the space environment is characterized by comprising the following steps:
step one, assembling the microfluidic perfusion culture module of claim 4, connecting a liquid storage unit, an injection pump unit and a culture detection chamber unit on a liquid separation control unit, and connecting leads of an electromagnetic valve, a heating film and a temperature sensor with an airtight electric connector;
step two, fixedly connecting the microfluidic perfusion culture module on the middle frame plate, wherein the fixation comprises a liquid storage bag, a waste liquid bag and a pipeline thereof;
step three, the injection pump penetrates through the middle frame plate, the O ring is sleeved outside the injection pump body, the injection pump is locked and fixed on the Ruhr connector, the O ring is fed into the sealing groove of the middle frame plate, the hollow sealing screw is sleeved and fixed outside the injection pump body, and the limiting block is clamped and connected with the push rod;
and fourthly, installing sealing rings in the annular sealing grooves on the inner side and the outer side of the middle frame plate, and connecting the upper cover plate and the lower cover plate with the periphery of the middle frame plate by using bolts.
CN202210808260.6A 2022-07-11 2022-07-11 Microfluidic perfusion culture integrated chip card box for space environment and preparation method thereof Pending CN115011473A (en)

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Application Number Priority Date Filing Date Title
CN202210808260.6A CN115011473A (en) 2022-07-11 2022-07-11 Microfluidic perfusion culture integrated chip card box for space environment and preparation method thereof
PCT/CN2023/093003 WO2024012029A1 (en) 2022-07-11 2023-05-09 Microfluidic perfusion culture integrated chip cartridge for space environment and preparation method therefor

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Application Number Priority Date Filing Date Title
CN202210808260.6A CN115011473A (en) 2022-07-11 2022-07-11 Microfluidic perfusion culture integrated chip card box for space environment and preparation method thereof

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CN115011473A true CN115011473A (en) 2022-09-06

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Publication number Priority date Publication date Assignee Title
WO2024012029A1 (en) * 2022-07-11 2024-01-18 北京理工亘舒科技有限公司 Microfluidic perfusion culture integrated chip cartridge for space environment and preparation method therefor

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