CN212128194U

CN212128194U - Based on cell culture device for biotechnology

Info

Publication number: CN212128194U
Application number: CN202020174278.1U
Authority: CN
Inventors: 袁楠楠; 韦金龙
Original assignee: Shaanxi Lejie Medical Technology Co ltd
Current assignee: Shaanxi Lejie Medical Technology Co ltd
Priority date: 2020-02-17
Filing date: 2020-02-17
Publication date: 2020-12-11
Anticipated expiration: 2030-02-17

Abstract

The utility model discloses a cell culture device for biotechnology based on, including sealed cabinet, it has sealing door to articulate on the sealed cabinet left side inner wall, fixedly connected with cell culture groove in the sealed cabinet, the interpolation of cell culture groove is equipped with stirring, the sealed cabinet is located and is equipped with the driving piece on the top surface that central point put, stirring and driving piece counterbalance contact, fixed mounting has the flowing back spare on the sealed cabinet bottom, the sealed cabinet is close to the equal fixedly connected with supporting shoe of lower extreme in four corners. The utility model discloses a connecting block and the connecting plate of joint rotate on the micro motor drives the fixed plate for connecting block and connecting plate carry out the intensive mixing to nutrient solution and cell sap in the cell culture groove, and the while is convenient carries out the dismouting to connecting block and connecting plate, avoids influencing the cultivation of cell sap, in time discharges the waste liquid that produces in with the cell culture groove via first pipe and second pipe through miniature negative pressure pump, avoids influencing the normal growth of cell.

Description

Based on cell culture device for biotechnology

Technical Field

The utility model relates to a cell culture technical field especially relates to a based on cell culture device for biotechnology.

Background

Biotechnology is a method for producing useful substances by applying the basic principles of biology, chemistry and engineering and utilizing organisms including microorganisms, animal cells and plant cells or their constituents such as organelles and enzymes, and cell culture is a method for simulating the environment of sterility, proper temperature, pH value and certain nutritional conditions in vitro to enable them to survive, grow, reproduce and maintain the main structure and function.

The existing cell culture device is not convenient for fully mixing nutrient solution and cell sap in the actual use process, and waste liquid generated in the cell culture process cannot be discharged in time to influence the normal growth of cells, so that the cell culture device for the biotechnology is provided to solve the problems.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a cell culture device based on biotechnology.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a cell culture device for biotechnology based, includes sealed cabinet, it has sealing door to articulate on the sealed cabinet left side inner wall, fixedly connected with cell culture groove in the sealed cabinet, the cell culture inslot interpolation is equipped with the stirring piece, the sealed cabinet is located and is equipped with the driving piece on the top surface that central point put, the stirring piece offsets with the driving piece and contacts, fixed mounting has the flowing back piece on the sealed cabinet bottom, the sealed cabinet is close to the equal fixedly connected with supporting shoe of lower extreme in four corners.

Preferably, the stirring piece is including inserting two connecting blocks of locating in the cell culture groove, two the connecting block all with driving piece activity joint, two equal fixedly connected with connecting plate on the relative lateral wall of connecting block, the connecting plate is close to the lower extreme setting of connecting block, the strip opening of intercommunication around being equipped with on the connecting plate.

Preferably, the driving piece includes that fixed connection is located the micro motor on the central point puts the top surface in the sealed cabinet, micro motor's the vertical setting down of drive shaft, fixedly connected with dwang in micro motor's the drive shaft, the lower extreme fixedly connected with fixed plate of dwang, be equipped with the rectangle that communicates from top to bottom on the fixed plate and lead to the groove, two the rectangle leads to the groove and is close to the setting of both ends lateral wall about the fixed plate respectively, two the connecting block is inserted respectively and is located two rectangles and lead to the inslot and contact with the upper end counterbalance of fixed plate.

Preferably, be equipped with two cavities in the connecting block, two the cavity all is close to the upper end setting of connecting block, two equal fixedly connected with spring on the relative inner wall of cavity, the one end fixedly connected with fixture block of cavity inner wall is kept away from to the spring, the fixture block runs through the setting of cavity inner wall and offsets the contact with the upper end of fixed plate.

Preferably, flowing back spare includes the miniature negative pressure pump of fixed mounting on the seal receptacle bottom, the input intercommunication of miniature negative pressure pump is provided with first pipe, the one end that miniature negative pressure pump was kept away from to first pipe sets up with the bottom intercommunication of cell culture groove, the output intercommunication of miniature negative pressure pump is provided with the second pipe, the one end that miniature negative pressure pump was kept away from to the second pipe is fixed to run through seal receptacle right side inner wall and is set up.

Preferably, the supporting block is made of rubber.

Compared with the prior art, the utility model, its beneficial effect does:

1. connecting block and the connecting plate of joint rotate on driving the fixed plate through micro motor for connecting block and connecting plate carry out the intensive mixing to nutrient solution and cell sap in the cell culture groove, and the while is convenient carries out the dismouting to connecting block and connecting plate, avoids influencing the cultivation of cell sap.

2. Waste liquid generated in the cell culture tank is discharged in time through the first conduit and the second conduit by the miniature negative pressure pump, so that the influence on the normal growth of cells is avoided.

Drawings

FIG. 1 is a schematic perspective view of a cell culture apparatus for biotechnology according to the present invention;

FIG. 2 is a schematic structural view of a stirring member of the cell culture apparatus for biotechnology according to the present invention;

fig. 3 is a partially enlarged view of a portion a in fig. 1.

In the figure: the device comprises a sealing cabinet 1, a sealing door 2, a cell culture tank 3, a stirring part 4, a driving part 5, a liquid discharging part 6, a supporting block 7, a connecting block 8, a connecting plate 9, a strip-shaped opening 10, a micro motor 11, a rotating rod 12, a fixing plate 13, a rectangular through groove 14, a cavity 15, a spring 16, a fixture block 17, a micro negative pressure pump 18, a first conduit 19 and a second conduit 20.

Detailed Description

In the description of the present invention, it is to be understood that the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention.

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-3, a cell culture device based on biotechnology, including seal cabinet 1, it has sealing door 2 to articulate on the inner wall of seal cabinet 1 left side, sealing door 2 can carry out effectual sealed to seal cabinet 1, avoid external environment to influence the growth of cell sap in cell culture groove 3, fixedly connected with cell culture groove 3 in seal cabinet 1, cell culture groove 3 interpolation is equipped with stirring piece 4, stirring piece 4 is including inserting two connecting blocks 8 of locating in cell culture groove 3, two connecting blocks 8 all with driving piece 5 activity joint, equal fixedly connected with connecting plate 9 on the relative lateral wall of two connecting blocks 8, connecting plate 9 is close to the lower extreme setting of connecting block 8, be equipped with the bar opening 10 of intercommunication around on the connecting plate 9, bar opening 10 is used for cooperating when connecting plate 9 rotates to carry out intensive mixing to nutrient solution and cell sap in cell culture groove 3.

Sealing cabinet 1 is located and is equipped with driving piece 5 on the top surface of central point position, driving piece 5 includes that fixed connection is in 1 micro motor 11 on the top surface of central point position of sealing cabinet, micro motor 11's drive shaft is vertical to be set up down, fixedly connected with dwang 12 in micro motor 11's the drive shaft, micro motor 11 is used for driving dwang 12 and rotates, micro motor 11 is prior art, do not describe here any more, the lower extreme fixedly connected with fixed plate 13 of dwang 12, be equipped with the logical groove 14 of rectangle that communicates about two on the fixed plate 13, two rectangles lead to the groove 14 and are close to the setting of both ends lateral wall about fixed plate 13 respectively, two connecting blocks 8 insert respectively and locate two rectangles lead to the inslot 14.

Stirring 4 and 5 looks butt contacts of driving piece, be equipped with two cavities 15 in the connecting block 8, two cavities 15 all are close to the upper end setting of connecting block 8, equal fixedly connected with spring 16 on two cavities 15's the relative inner wall, the one end fixedly connected with fixture block 17 of 15 inner walls of cavity is kept away from to spring 16, fixture block 17 runs through 15 inner walls of cavity setting and with the upper end looks butt contact of fixed plate 13, the upper end looks butt contact of fixture block 17 lower extreme and fixed plate 13, can locate connecting block 8 in the logical groove 14 of rectangle to inserting and carry out spacing fixed.

The liquid drainage part 6 is fixedly arranged on the bottom of the sealing cabinet 1, the liquid drainage part 6 comprises a micro negative pressure pump 18 fixedly arranged on the bottom of the sealing cabinet 1, the micro negative pressure pump 18 is in the prior art, details are not described herein, an input end of the micro negative pressure pump 18 is communicated with a first conduit 19, a valve is arranged on the first conduit 19 and used for controlling the flow of the first conduit 19, the valve is in the prior art and not described herein, one end of the first conduit 19, which is far away from the micro negative pressure pump 18, is communicated with the bottom of the cell culture tank 3, an output end of the micro negative pressure pump 18 is communicated with a second conduit 20, one end of the second conduit 20, which is far away from the micro negative pressure pump 18, is fixedly arranged through the inner wall of the right side of the sealing cabinet 1, the micro negative pressure pump 18 is used for timely discharging waste liquid generated in the cell culture tank 3 by matching with the first conduit 19, the lower ends of the sealing cabinet 1 close to the four corners are fixedly connected with supporting blocks 7, and the supporting blocks 7 are made of rubber.

When the utility model is used, cell fluid to be cultured is guided into the sterile cell culture tank 3, nutrient solution is added into the cell culture tank 3, then two fixture blocks 17 of two connecting blocks 8 are manually pressed to enable the fixture blocks 17 to compress the spring in the cavity 15 and partially contract to the cavity 15 until the connecting blocks 8 can be inserted into the rectangular through grooves 14 on the fixing plate 13, after the fixture blocks 17 on the connecting blocks 8 are moved out of the rectangular through grooves 14, the spring 16 is reset to drive the fixture blocks 17 to be moved out of the cavity 15 and to be abutted against and contacted with the upper end of the fixing plate 13, so that the two fixture blocks 17 can limit and fix the connecting blocks 8 in the rectangular through grooves 14, at the moment, the micro motor 11 is started to drive the rotating rod 12 to rotate, the rotating rod 12 drives the two fixed connection blocks 8 on the fixing plate 13 to rotate, the connecting blocks 8 drive the connecting plate 9 to rotate, and the nutrient solution and the cell fluid in the, after mixing the completion, press fixture block 17 on the connecting block 8 once more, and pull connecting block 8 and make its and the separation of rectangle through groove 14, can take out connecting block 8 and connecting plate 9 in the cell culture groove 3, avoid influencing the cultivation of cell sap, make the cell sap normal growth in the cell culture groove 3 after closing sealing door 2, when the waste liquid that produces in the cell culture groove 3 needs to be got rid of, only need start miniature negative pressure pump 18 in time to discharge the waste liquid that produces in the cell culture groove 3 via first pipe 19 and second pipe 20, avoid influencing the normal growth of cell.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. The utility model provides a cell culture device for biotechnology based on, includes sealed cabinet (1), it has sealing door (2) to articulate on the inner wall of sealed cabinet (1) left side, a serial communication port, fixedly connected with cell culture groove (3) in sealed cabinet (1), cell culture groove (3) interpolation is equipped with stirring piece (4), sealed cabinet (1) is located and is equipped with driving piece (5) on the top surface that central point put, stirring piece (4) and driving piece (5) counterbalance contact, fixed mounting has flowing back piece (6) on sealed cabinet (1) bottom, sealed cabinet (1) is close to equal fixedly connected with supporting shoe (7) of lower extreme in four corners.

2. The cell culture device for biotechnology based on of claim 1, wherein the stirring member (4) comprises two connecting blocks (8) inserted in the cell culture tank (3), the two connecting blocks (8) are movably clamped with the driving member (5), the two connecting blocks (8) are fixedly connected with connecting plates (9) on opposite side walls, the connecting plates (9) are arranged near the lower ends of the connecting blocks (8), and the connecting plates (9) are provided with strip-shaped openings (10) which are communicated with each other from front to back.

3. The cell culture device for biotechnology based on according to claim 2, wherein, driving piece (5) includes that fixed connection is in the micro motor (11) on the top surface of central point is located to sealed cabinet (1), the drive shaft of micro motor (11) sets up vertically down, fixedly connected with dwang (12) in the drive shaft of micro motor (11), the lower extreme fixedly connected with fixed plate (13) of dwang (12), be equipped with two rectangle through grooves (14) that communicate from top to bottom on fixed plate (13), two rectangle through groove (14) are close to the left and right sides both ends lateral wall setting of fixed plate (13) respectively, two connecting block (8) are inserted respectively and are located two rectangle through grooves (14) and with the upper end looks butt of fixed plate (13).

4. The cell culture device for the biotechnology-based cell culture according to claim 3, wherein two cavities (15) are arranged in the connecting block (8), the two cavities (15) are both arranged near the upper end of the connecting block (8), springs (16) are fixedly connected to the opposite inner walls of the two cavities (15), a clamping block (17) is fixedly connected to one end, far away from the inner wall of the cavity (15), of each spring (16), and the clamping block (17) penetrates through the inner wall of the cavity (15) and is in contact with the upper end of the fixing plate (13) in an abutting mode.

5. The cell culture device for biotechnology based on claim 1, wherein the draining part (6) comprises a micro negative pressure pump (18) fixedly installed on the bottom of the sealing cabinet (1), the input end of the micro negative pressure pump (18) is provided with a first conduit (19) in a communicating manner, one end of the first conduit (19) far away from the micro negative pressure pump (18) is provided with a communicating manner on the bottom of the cell culture tank (3), the output end of the micro negative pressure pump (18) is provided with a second conduit (20) in a communicating manner, and one end of the second conduit (20) far away from the micro negative pressure pump (18) is fixedly arranged through the inner wall of the right side of the sealing cabinet (1).

6. The device as claimed in claim 1, wherein the supporting block (7) is made of rubber.