CN1989831B - Microorganism feed addictive improving reproduction ability of boar and making method thereof - Google Patents
Microorganism feed addictive improving reproduction ability of boar and making method thereof Download PDFInfo
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- CN1989831B CN1989831B CN2005101121305A CN200510112130A CN1989831B CN 1989831 B CN1989831 B CN 1989831B CN 2005101121305 A CN2005101121305 A CN 2005101121305A CN 200510112130 A CN200510112130 A CN 200510112130A CN 1989831 B CN1989831 B CN 1989831B
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- bacillus subtilis
- lactobacillus lactis
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Abstract
The invention involves a microbial feed additive and its prepare methods which improveboar fecundity, it is characterized by:lactic acid lactobacillus and bacillus subtilis which are optimized make the products rich in beneficial microbios and variety of active metabolite through the means of combining pedigree breed fermentation and composite fermentation. The advantages of the invention are maintaining boar body health,repairing tissue injury, improving the fecundity and the body immune function, thus making the boar maintain strong sexuality and produce high grade semen, extending boar life, and nontoxic, nonpersistent and the use is convenience.
Description
Technical field
The present invention relates to a kind of additive for microbe feedstuff and preparation method that improves reproduction ability of boar, belong to field of feed additive technology.
Background technology
Produce the quality of herd boar, particularly semen quality directly influences the reproductive performance of sow, current in breed, exist excessively use, factors such as nutrition is unreasonable, disease puzzlement, feeding environment pollution, thereby directly influence the boar semen quality, cause the breeding conception rate low, economic benefit improves not obvious.Raise in the hurdle house because boar is long-term, lack the outer sports ground of house, the frequency of utilization height, cause the boar of considerable part early ageing to occur, mounting is unable, and sexual reflex is slow during mating, semen quality and quantity descend, and degradation under the breeding conception rate has had a strong impact on the productivity on pig farm.
The application's patent is intended to improve the raising of the production capacity of herd boar, particularly semen quality, and the reproductive performance of sow also is improved; Improve the anti-stress ability of boar simultaneously, prolong good boar service life, the outstanding proterties of good boar is enlarged to greatest extent.
Summary of the invention
The additive for microbe feedstuff and the preparation method that the purpose of this invention is to provide a kind of raising reproduction ability of boar that combines by pure-blood ferment and composite fermentation by the beneficial bacterium of optimizing, make the feed addictive that obtains after the fermentation except containing a large amount of beneficial microbes, also comprise the various active composition, as Bacillus acidi lactici peptide glycan, amino acid, vitamin, various active enzyme, polypeptide, and multiple natural bacteriostatic material such as organic acid, bacteriocin, hydrogen peroxide, biacetyl.In application, by organic microbial in the product and multiple fermentating metabolism product, systematically improve the body function, damaged tissue repair improves anti-stress ability, thereby makes boar keep vigorous sexual desire ability, and production high-quality seminal fluid improves the breeding conception rate; And product is nontoxic, noresidue, and is easy to use.
For realizing above purpose, technical scheme of the present invention provides a kind of additive for microbe feedstuff that improves reproduction ability of boar, it is characterized in that, carry out liquid and solid culture respectively by lactobacillus lactis, bacillus subtilis, then culture is proportionally mixed, after passing through anaerobic fermentation again, drying and crushing obtains finished product
Lactobacillus lactis carries out Liquid Culture, and the percentage by weight of its raw material preparation is as follows:
Lactobacillus lactis bacterium liquid 10-16%,
Fluid nutrient medium 83-91%;
Bacillus subtilis carries out solid fermentation, and the percentage by weight of its raw material preparation is as follows:
Bacillus subtilis bacterium liquid 8-15%,
Culture medium 35-61%,
Water 30-49%;
The ratio that the lactobacillus lactis culture mixes with the bacillus subtilis culture is 1: 3-4 (by weight).
Described lactobacillus lactis culture medium is in dregs of beans, corn steep liquor, glucose, yeast extract, urea, ammonium sulfate, epsom salt, potassium dihydrogen phosphate, the water at least six kinds, and its percentage by weight is dregs of beans 1-5%, corn steep liquor 0-2%, glucose 0.5-2%, yeast extract 0.5-1.5%, urea 0-0.8%, ammonium sulfate 0-0.04%, epsom salt 0.02-0.1%, potassium dihydrogen phosphate 0.01-0.1%, water 89-96%.
Described bacillus subtilis bacterium culture medium is wheat bran, rice husk, corn flour, dregs of beans, magnesium sulfate, manganese sulfate, and its percentage by weight is wheat bran 70-90%, rice husk 5-15%, corn flour 2-8%, dregs of beans 2-8%, magnesium sulfate 0.01-0.1%, manganese sulfate 0.005-0.02%.
Improve the preparation method of the additive for microbe feedstuff of reproduction ability of boar, it is characterized in that, lactobacillus lactis and bacillus subtilis adopt traditional method for culturing and separating to screen, and adopt pure-blood ferment and composite fermentation two-stage process simultaneously, and it comprises the following steps:
The first step: in the meat soup culture medium of respectively bacterial classification being transferred, after cultivating 22～26 hours under 30～37 ℃ of temperature, preserved after the separation that the selection colony characteristics obviously, the vigor of examining under a microscope is strong and the fast bacterium colony of growth and breeding is rule;
Second step: lactobacillus lactis is inoculated in the fluid nutrient medium, and pH is adjusted to 5.0, and cultivation temperature is that 30～35 ℃, time are 20～24 hours, obtains the lactobacillus lactis culture;
The 3rd step: bacillus subtilis is inoculated in the solid medium, and pH is adjusted to 6.4, and cultivation temperature is that 35～39 ℃, time are 38～45 hours, obtains the bacillus subtilis culture;
The 4th step: lactobacillus lactis culture and bacillus subtilis culture are according to 1: the ratio of 3-4 mixes, and inserts in the ferment at constant temperature room after airtight, and cultivation temperature is 30-35 ℃, and the time is 84-96 hour;
The 5th step: with the low temperature drying of ventilating of the product after the fermentation ends, baking temperature is 35-40 ℃, and the time is 6-8 hour, and final moisture is controlled in 11%;
The 6th step: will obtain the sharp smart health feed addictive finished product of pulverous microorganism boar after the dried product pulverizing.
The present invention adopts lactobacillus lactis, the bacillus subtilis through optimization process, the mode that combines by pure-blood ferment and composite fermentation, make and be rich in beneficial microbe and various active metabolite in the product, as Bacillus acidi lactici peptide glycan, amino acid, vitamin, various active enzyme, polypeptide, and multiple natural bacteriostatic material such as organic acid, bacteriocin, hydrogen peroxide, biacetyl.In application, by organic microbial in the product and multiple fermentating metabolism product, systematically improve the body function, damaged tissue repair improves anti-stress ability, thereby makes boar keep vigorous sexual desire ability, and production high-quality seminal fluid improves the breeding conception rate; And product is nontoxic, noresidue, and is easy to use.
Gonad development and the sexual function of buck mainly is subjected to the regulation and control of " hypothalamus-hypophysis-testis axle ", the sexual function of boar is relevant with the basic metabolism of self, thyroid gland is one of key factor that influences basic metabolism, the seminal fluid that thyroid secretion not only influences boar also influences sexual function and the sexual reflex of boar, basic metabolism is low not only to be influenced sexual function boar is fleshed up, and then influencing sexual desire, sex hormone is to influence boar sexual function and gonepoietic another key factor.The androgen consumption is crossed conference influences boar self endocrine, causes boar too to rely on exogenous hormone.The intramuscular injection of generally adopting at present tends to cause mandatory a large amount of androgen to enter in the boar body, cause boar endocrine confusion, sexual function descends, and the sharp smart health feed addictive of microorganism boar mainly is to reach by the active metabolite that promotes the boar hormone secretion to improve male animal sexual desire purpose.
Contain a large amount of O.P.C (natural anti-reflecting oxide) in the oxygen radical microorganism scavenger and have superpower antioxidation, can promote immune organ to grow, remove free radical, the multiple difficult and complicated cases of effectively preventing boar to cause owing to free radical infringement, immunologic hypofunction etc.
Can cause during hypovitaminosis that testis degeneration seminal fluid reduces, sperm motility descends, degradation phenomenon under the sexual desire.And contain abundant vitamin A, D, E in the sharp smart health of microorganism boar, have the gonopoiesis of raising amount, improve the reproduction function, improve semen quality, the facilitation that is formed with of sperm; Also contain a large amount of amino acid, enzyme and other metabolite in the sharp smart health simultaneously, all have the sexual desire of reinforcement, improve motility of sperm, promote the stosterone secretion, strengthen ejaculate volume, increase sperm concentration, and the effect that reduces rate of teratosperm, prevention orchiatrophy.
In the lactobacter growth metabolic process, can produce the material that some have antimicrobial acivity, as: organic acid, hydrogen peroxide, carbon dioxide etc., all at the external bacteriostatic activity that shows.A lot of lactic acid bacterias can both produce bacteriocin, as: nisalpin, lactein, bite sour rhzomorph etc., show that after deliberation these materials are suppressing to have important function on the pathogen.Beneficial microbe in the sharp smart health feed addictive of microorganism boar in conjunction with original beneficial bacterium, forms the superior microorganism flora rapidly after entering animal intestinal, suppressed growth of harmful intestinal tract bacteria, grows to it and has created a stable environment; By a large amount of beneficial microorganism effects, active principle in the feed is converted into the various amino acid that absorbed by animal easily, organic acid, polysaccharide, multivitamin and polyphenoils etc., make animal set up optimum little ecosystem, improved the combination of animal intestinal micropopulation, suppress the growth of enteron aisle corruptibility bacterium, promote the Animal nutrition absorption function, ensure the nutritional need of boar at each physiological stage, avoid boar situations such as overfertilization or subalimentation to occur, influence its reproductive performance.
The sharp smart health feed addictive of microorganism boar can strengthen the immunity of boar, show both ways: the one, influence nonspecific immune response, strengthen the vigor of MNP (monocyte and macrophage), polymorphonuclear leukocyte, the secretion of stimulating activity oxygen, lysosomal enzyme and monokine; The 2nd, stimulate specific immune response, as IgA and IgM, IgG level in reinforcement mucomembranous surface and the serum, promote the propagation of T, bone-marrow-derived lymphocyte, strengthen the immunity of body.
Advantage of the present invention is:
1. changed and single in the past utilized hormone, medicine to improve the boar sexual desire and improve the quality of seminal fluid with the amount that increases vitamin, certain trace element;
2. improve the immunocompetence of boar, reduce the generation of disease;
3. can repair damage body tissue, excite reproductive hormone to improve the output of seminal fluid;
4. the enhancing anti-stress ability prolongs the service life of boar, reduces and eliminates ratio, increases economic benefit;
5. nontoxic, the noresidue of this product, easy to use.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail:
Embodiment 1
Improve the preparation method of the additive for microbe feedstuff of reproduction ability of boar, the bacterial classification of employing all comes by screening and optimizing, adopts pure-blood ferment and composite fermentation process combined simultaneously, and it comprises the following steps:
The first step: in the meat soup culture medium of respectively bacterial classification being transferred, after 24 hours, preserved after the separation that the selection colony characteristics obviously, the vigor of examining under a microscope is strong and the fast bacterium colony of growth and breeding is rule in cultivation under 35 ℃ of temperature;
Second step: lactobacillus lactis is inoculated in the fluid nutrient medium, fluid nutrient medium consists of: dregs of beans 3%, glucose 1%, yeast extract 1%, epsom salt 0.05%, potassium dihydrogen phosphate 0.03%, water 94.92%, pH is adjusted to 5.0, cultivation temperature is that 30 ℃, time are 20h, obtains the lactobacillus lactis culture;
The 3rd step: bacillus subtilis is inoculated in the solid medium, solid medium consists of: wheat bran 80%, rice husk 10%, corn flour 5%, dregs of beans 4.94%, magnesium sulfate 0.05%, manganese sulfate 0.01%, pH is adjusted to 6.4, cultivation temperature is 37 ℃, time is 40 hours, obtains the bacillus subtilis culture;
The 4th step: lactobacillus lactis culture and bacillus subtilis culture mix according to 1: 3.5 ratio, insert in the ferment at constant temperature room after airtight, and cultivation temperature is 32 ℃, and the time is 96 hours;
The 5th step: with the low temperature drying of ventilating of the product after the fermentation ends, baking temperature is 40 ℃, and the time is 8 hours, and final moisture is controlled in 11%;
The 6th step: will obtain the sharp smart health feed addictive finished product of pulverous microorganism boar after the dried product pulverizing.
Embodiment 2:
The first step: in the meat soup culture medium of respectively bacterial classification being transferred, after 24 hours, preserved after the separation that the selection colony characteristics obviously, the vigor of examining under a microscope is strong and the fast bacterium colony of growth and breeding is rule in cultivation under 35 ℃ of temperature;
Second step: lactobacillus lactis is inoculated in the fluid nutrient medium, fluid nutrient medium consists of: dregs of beans 4%, corn steep liquor 2%, glucose 1.2%, yeast extract 1.2%, epsom salt 0.03%, potassium dihydrogen phosphate 0.06%, water 91.5%, pH is adjusted to 5.0, cultivation temperature is 35 ℃, time is 24 hours, obtains the lactobacillus lactis culture;
The 3rd step: bacillus subtilis is inoculated in the solid medium, solid medium consists of: wheat bran 74%, rice husk 12%, corn flour 6%, dregs of beans 7.94%, magnesium sulfate 0.05%, manganese sulfate 0.01%, pH are adjusted to 6.4, cultivation temperature is 39 ℃, time is 38 hours, obtains the bacillus subtilis culture;
The 4th step: lactobacillus lactis culture and bacillus subtilis culture mix according to 1: 4 ratio, insert in the ferment at constant temperature room after airtight, and cultivation temperature is 35 ℃, and the time is 84 hours;
The 5th step: with the low temperature drying of ventilating of the product after the fermentation ends, baking temperature is 40 ℃, and the time is 6 hours, and final moisture is controlled in 11%;
The 6th step: will obtain the sharp smart health feed addictive finished product of pulverous microorganism boar after the dried product pulverizing.
In culturing application, concrete using method and use amount are as follows: add 800g-1200g by feed per ton, feeding after mixing gets final product; In weather hot season in 6 to October, can on original addition, increase the consumption of 10-20%.
Claims (1)
1. an additive for microbe feedstuff that improves reproduction ability of boar is characterized in that, carries out liquid and solid culture respectively by lactobacillus lactis, bacillus subtilis, then culture is proportionally mixed, after passing through anaerobic fermentation again, drying and crushing obtains finished product
Lactobacillus lactis carries out Liquid Culture, and the percentage by weight of its raw material preparation is as follows:
Lactobacillus lactis bacterium liquid 10-16%,
Fluid nutrient medium 83-91%; Two kinds of raw material sums are 100%;
Bacillus subtilis carries out solid fermentation, and the ratio of its raw material preparation is as follows:
Bacillus subtilis bacterium liquid 8-15%,
Culture medium 35-61%,
Water 30-49%; Three kinds of raw material sums are 100%;
The ratio that the lactobacillus lactis culture mixes with the bacillus subtilis culture is 1: 3～4 by weight;
Described lactobacillus lactis culture medium is in dregs of beans, corn steep liquor, glucose, yeast extract, urea, ammonium sulfate, epsom salt, potassium dihydrogen phosphate, the water at least six kinds, and its percentage by weight is dregs of beans 1～5%, corn steep liquor 0～2%, glucose 0.5～2%, yeast extract 0.5～1.5%, urea 0～0.8%, ammonium sulfate 0～0.04%, epsom salt 0.02～0.1%, potassium dihydrogen phosphate 0.01～0.1%, water 89～96%;
Described bacillus subtilis bacterium culture medium is wheat bran, rice husk, corn flour, dregs of beans, magnesium sulfate, manganese sulfate, and its percentage by weight is wheat bran 70～90%, rice husk 5～15%, corn flour 2～8%, dregs of beans 2～8%, magnesium sulfate 0.01～0.1%, manganese sulfate 0.005～0.02%;
The preparation method that improves the additive for microbe feedstuff of reproduction ability of boar is, lactobacillus lactis and bacillus subtilis adopt traditional method for culturing and separating to screen, and adopt pure-blood ferment and composite fermentation two-stage process simultaneously, and it comprises the following steps:
The first step: in the meat soup culture medium of respectively bacterial classification being transferred, after cultivating 22～26 hours under 30～37 ℃ of temperature, preserved after the separation that the selection colony characteristics obviously, the vigor of examining under a microscope is strong and the fast bacterium colony of growth and breeding is rule;
Second step: lactobacillus lactis is inoculated in the fluid nutrient medium, and pH is adjusted to 5.0, and cultivation temperature is that 30-35 ℃, time are 20-24 hour, obtains the lactobacillus lactis culture;
The 3rd step: bacillus subtilis is inoculated in the solid medium, and pH is adjusted to 6.4, and cultivation temperature is that 35-39 ℃, time are 38-45 hour, obtains the bacillus subtilis culture;
The 4th step: lactobacillus lactis culture and bacillus subtilis culture are according to 1: the ratio of 3-4 mixes, and inserts in the ferment at constant temperature room after airtight, and cultivation temperature is 30-35 ℃, and the time is 84-96 hour;
The 5th step: with the low temperature drying of ventilating of the product after the fermentation ends, baking temperature is 35-40 ℃, and the time is 6-8 hour, and final moisture is controlled in 11%;
The 6th step: will obtain the additive for microbe feedstuff finished product of pulverous raising reproduction ability of boar after the dried product pulverizing.
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| CN2005101121305A CN1989831B (en) | 2005-12-28 | 2005-12-28 | Microorganism feed addictive improving reproduction ability of boar and making method thereof |
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| CN1989831B true CN1989831B (en) | 2011-06-08 |
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Families Citing this family (11)
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| CN101301402B (en) * | 2008-07-03 | 2011-07-20 | 刘庆华 | Nutrition health care replenisher for reinforcing reproduction performance of boar |
| FR2958165B1 (en) * | 2010-03-30 | 2012-08-03 | Agronomique Inst Nat Rech | METHOD FOR STIMULATING REPRODUCTIVE CAPACITY IN BULL AND COMPOSITION FOR STIMULATING REPRODUCTIVE CAPACITY IN BULL |
| CN101869222B (en) * | 2010-06-08 | 2012-06-27 | 浙江大学 | Feed additive for improving reproductive capacity of boar and preparation method thereof |
| CN101953430B (en) * | 2010-07-15 | 2013-05-22 | 北京龙科方舟生物工程技术有限公司 | Feed, preparation method and use thereof in improvement in reproductive performance of sows |
| CN102178110B (en) * | 2011-05-12 | 2012-10-17 | 比利美英伟营养饲料（深圳）有限公司 | Premix feed for improving reproductive performance of boar |
| CN102308926B (en) * | 2011-08-23 | 2013-03-20 | 安徽宝迪肉类食品有限公司 | Feed additive capable of treating boar sand crack effectively |
| CN102696916A (en) * | 2012-06-02 | 2012-10-03 | 上海创博饲料科技有限公司 | Compound fermentation method for producing fermented feed |
| CN104855672B (en) * | 2014-02-26 | 2018-07-27 | 哈尔滨龙猪科技开发有限公司 | Rhzomorph pellet preparation process |
| CN106819495B (en) * | 2017-04-13 | 2020-07-14 | 湖南赛福资源饲料科技有限公司 | Replacement boar feed and preparation method thereof |
| CN107041475A (en) * | 2017-05-23 | 2017-08-15 | 厦门创和美饲料科技有限公司 | Improve the processing technology of the core feed additives of reproduction ability of boar and the concentrated feed containing the additive and the concentrated feed |
| CN111296658B (en) * | 2017-09-05 | 2024-03-12 | 长春禾丰饲料有限责任公司 | Compound feed for improving reproductive performance of breeding boars and preparation method thereof |
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