CN101869222B - Feed additive for improving reproductive capacity of boar and preparation method thereof - Google Patents

Feed additive for improving reproductive capacity of boar and preparation method thereof Download PDF

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CN101869222B
CN101869222B CN201010195505XA CN201010195505A CN101869222B CN 101869222 B CN101869222 B CN 101869222B CN 201010195505X A CN201010195505X A CN 201010195505XA CN 201010195505 A CN201010195505 A CN 201010195505A CN 101869222 B CN101869222 B CN 101869222B
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vitamin
boar
percent
feed additive
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CN101869222A (en
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王友明
姚中磊
楼永波
靳明亮
周金伟
王世杰
陈大水
沈建平
缪成泉
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Zhejiang University ZJU
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Abstract

The invention discloses a feed additive for improving the reproductive capacity of a boar. The feed additive comprises the following raw materials in percentage by weight: 40 to 50 percent of epimedium, 8 to 10 percent of cynomorium songaricum, 8 to 10 percent of codonopsis pilosula, 8 to 10 percent of angelica sinensis, 8 to 10 percent of medlar, 0.5 to 1 percent of vitamin A, 0.1 to 0.2 percentof vitamin D3, 1 to 4 percent of vitamin E, 0.1 to 0.3 percent of vitamin B2 and the balance of zeolite powder. A preparation method for the feed additive comprises the following steps of: mixing the epimedium, the cynomorium songaricum, the codonopsis pilosula, the angelica sinensis and the medlar; smashing a mixture to a particle size of between 25 and 40 microns; adding the vitamin A, the vitamin D3, the vitamin E, the vitamin B2 and the zeolite powder; and mixing uniformly. The feed additive prepared by the method has the characteristics of remarkable improvement on boar sexual desire, ejaculatory amount, sperm concentration, survival time and oestrum conception rate without any adverse effect, less additive amount, low cost and obvious effect.

Description

A kind of feed addictive that improves reproductive capacity of boar and preparation method thereof
Technical field
The present invention relates to a kind of feed addictive, particularly relate to a kind of feed addictive that improves reproductive capacity of boar and preparation method thereof
Background technology
The quality of boar directly influences the fertility of sow in the production, and current boar frequency of utilization height, the factors such as nutrition is unreasonable, disease is many, feeding environment difference of in aquaculture, existing cause the boar sexual desire to descend; Mounting is unable, and sexual reflex is slow during mating, and ejaculate volume reduces; The semen quality variation, even reproductive performance obstacles such as sperm is extremely rare, no sperm appear, cause the breeding conception rate low; Economic benefit descends, and has had a strong impact on the productivity on pig farm.The reproduction ability of boar obstacle roughly has three kinds of situation: sexual hypoesthesia or forfeiture; Sexual desire is arranged, but not mounting sow, can not mating; Sexual desire is arranged, but cacospermia, noncapacitation power.
All the time, be intramuscular injection sex hormone to the counter-measure of reproduction ability of boar obstacle, hormone villous film promoting sexual gland hormone, pregnant mare serum gonadotrop(h)in (PMSG) and testosterone propionate commonly used.But use the injectivity hormone inconvenient in the production, can cause the boar stress reaction, upset the normal physiological endocrine of boar; And the sex hormone function time is short, needs to inject a couple of days continuously.If for a long time, the usability hormone possibly cause the negative effects such as property paralysis of boar continually.And the injectivity hormone also can only temporarily strengthen the sexual behaviour of boar, can not fundamentally solve the reproduction ability of boar obstacle, quickens the boar reproductive function on the contrary and degenerates, and shortens boar and utilizes the time limit.
Theory of traditional Chinese medical science is thought; Kidney is the origin that body produces cloudy liquid and yang-energy, and modern physiology thinks that also kidney is very important, has multiple secreting function; And think that sperm is to be formed by the spermatogonium on the selected songs tubule of testis; Sperm generates and receives multiple hormone regulating and controlling, comprises energy with trophic level, and especially protein, trace element, vitamin etc. are closely related.The frequent semen collection, it is too much to cause the boar qi and blood to lose, empty retardance, kidney yang deficiency, the endocrine disturbance of losing, nutriment is unable to make ends meet, thereby hypogona dism.
For this reason, part scholar changes the research thinking, sets about utilizing Chinese herbal medicine to improve boar sexual desire and fecundity, has obtained certain effect.The bibliographical information Chinese herb feed additive has functions such as prevent disease, blood yiqi, mediation gaseous pulse, tonifying kidney and strengthening yang; Use the back to the animals and animal product noresidue, have no side effect; Be difficult for developing immunity to drugs (Chinese herb feed additive is to the influence of quality of boar semen. Long Xiang etc. and Sichuan animal and veterinary, the 3rd phase .1998).From the modern nutriology theory analysis, the contained alkaloid of Chinese herbal feed additive, polysaccharide, glucoside, volatile fatty acid, organic acid etc. all are bioactivators, to improving boar sexual desire and reproductive performance good action are arranged.Have the scholar (the Chinese herbal medicine composite feed additive is to the research of quality of boar semen influence. the ugly life of Liu. the Heilungkiang animal reproduction; The 1st phase .1994) though utilizes Chinese herbal treatment reproduction ability of boar obstacle to obtain certain effect; The additive addition is high, drug effect is slow, waste problems such as serious and palatability difference but exist; Thereby cause boar to produce few food or food refusal behavior, do not reach desirable effect.
Summary of the invention
When the invention provides a kind of the use addition few, have no side effect and obviously improve the Chinese herbal medicine composite feed additive of boar sexual desire and fecundity.
A kind of feed addictive that improves reproductive capacity of boar, its raw material weight percentage consists of:
Barrenwort 40~50%
Cynomorium songaricum 8~10%
Radix Codonopsis 8~10%
Radix Angelicae Sinensis 8~10%
Matrimony vine 8~10%
Vitamin A 0.5~1%
Vitamin D 30.1~0.2%
Vitamin E 1~4%
Cobastab 20.1~0.3%
All the other are zeolite powder.
Preferably, its raw material weight percentage consists of:
Barrenwort 40~50%
Cynomorium songaricum 8~10%
Radix Codonopsis 8~10%
Radix Angelicae Sinensis 8~10%
Matrimony vine 8~10%
Vitamin A 1%
Vitamin D 30.2%
Vitamin E 4%
Cobastab 20.3%
All the other are zeolite powder.
Barrenwort: warm in nature, flavor is hot, sweet, returns liver, kidney channel, has kidney-replenishing, strengthing aitch bone, and the wet effect of dispeling the wind is used to treat impotence and seminal emission, coils diseases such as the atrophic debility of bones is soft, arthralgia due to wind-dampness, numbness contracture.
Cynomorium songaricum: warm in nature, it is sweet, puckery to distinguish the flavor of, and returns liver, kidney, large intestine channel, has the establishing-Yang controlling nocturnal emission with astringent drugs, and the effect of the strong muscle that nourishes blood is used to treat impotence and premature ejaculation, the weak deficiency of Yin of gas, dry feces, soreness and weakness of waist and knees, fatigue and weak.
Radix Codonopsis: property is flat, and spleen, lung channel are returned in sweet, little acid of distinguishing the flavor of, and have tonifying middle-Jiao and Qi, and the effect of invigorating the spleen benefit lung is used to treat a little less than the spleen deficiency syndrome of the lung, the palpitaition of breathing hard, and anorexia and loose stool, void is breathed with cough and is coughed, and interior heat is quenched one's thirst.
Radix Angelicae Sinensis: warm in nature, it is sweet, hot to distinguish the flavor of, and returns liver, the heart, the spleen channel, and having enriches blood invigorates blood circulation, and the effect that relaxes bowel cures mainly sallow, the dizzy palpitaition of the deficiency of blood, asthenia cold abdominalgia, the dry constipation of intestines, arthralgia due to wind-dampness.
Matrimony vine: property is flat, and it is sweet distinguish the flavor of, returns liver, kidney, lung channel, has nourishing liver and kidneyly, and the effect of benefiting shrewd head cures mainly that consumptive disease is smart to be lost, soreness of waist and knee joint, and dizzy tinnitus, interior heat is quenched one's thirst, and the deficiency of blood is sallow, and blurred vision is failed to understand.
Above-mentioned Chinese medicine all can be selected from the commercially available prod.
Vitamin A may be selected to be the vitamin A micro-capsule of 500,000 IU/ gram.
Vitamin D 3May be selected to be the vitamin D of 500,000 IU/ gram 3Micro-capsule.
Vitamin E may be selected to be 50% vitamin E powder.
Cobastab 2May be selected to be 80% Cobastab 2Micro-capsule.
Vitamin A, D 3, E, B 2All can be selected from the commercially available prod.
The fineness of zeolite powder is 80~120 orders.
Vitamin A, D 3, E, B 2Deng all being the important nutrient that sperm forms; Barrenwort, cynomorium songaricum, Radix Codonopsis, Radix Angelicae Sinensis, matrimony vine and said vitamin coordination linkage can improve the boar sexual desire significantly, improve ejaculate volume, sperm concentration, time-to-live and non-return rate, do not have any harmful effect.When boar is deficient in vitamin A, testis tissue can occurs and degenerate, sexual desire descends, and semen quality is poor, lack of quantity of spermatic fluid, thin, density is not high; D is deficient in vitamin 3The time, can influence quality of boar semen, and influence is to the normal absorption of calcium, phosphorus; When being deficient in vitamin E, can cause the boar sexual desire to reduce, semen volume reduces, and sperm motility descends, orchiatrophy aspermatogenesis when serious; B is deficient in vitamin 2The time, orchiatrophy and metaboly can appear.
Chinese herbal medicine composite feed additive of the present invention in use, the percentage by weight of additive in feed is: 0.08~0.12%.
The preparation method of above-mentioned feed addictive comprises: with percentage by weight is that 40~50% barrenwort, 8~10% cynomorium songaricum, 8~10% Radix Codonopsis, 8~10% Radix Angelicae Sinensis and 8~10% matrimony vine mix, and being crushed to particle diameter is 25~40 microns; Adding percentage by weight is 0.5~1% vitamin A, 0.1~0.2% vitamin D 3, 1~4% vitamin E, 0.1~0.3% Cobastab 2And zeolite powder, mix.
Broken being meant with the biological cell broken wall of cell grade micro mist is the comminution process of purpose, and it is not purpose with the smashing fineness, but pursues the sporoderm-broken rate of cell.Broken through the cell grade micro mist, can the fineness of former green material be brought up to more than 300 orders from 40~80 orders of traditional handicraft, for general medicinal material, the breaking-wall cell rate under this fineness condition is greater than 95%.Cell intracellular active ingredient behind broken wall fully comes out, the rate of release and the burst size of medicine can significantly improve.The medicinal material particle is after the cell grade micro mist is broken, and microscopically is observed only has the minute quantity intact cell to exist.The cell grade micro mist is broken to mainly contain following advantage aborning: (1) increases the drug absorption rate, and improve the Chinese traditional medicine biology utilization rate: after Chinese medicine cell grade micro mist was broken, the main pharmacodynamics composition of medicine was distributed in cell and the cytoplasm usually, and being main in the cell.Active ingredient comes out in its cell behind the breaking-wall cell, so drug effect increases substantially, and onset speed is fast; (2) help the retains biological activity composition; Improve drug effect: in the broken process of cell grade micro mist; Can avoid superheating phenomenon through control, even can under low-temperature condition, carry out, it is fast to add pulverizing speed; Help keeping the bioactive ingredients and the various nutritional labeling of non-refractory like this, thereby improve drug effect; (3) conservation, be convenient to use: Chinese medicine can use the dosage littler than former prescription medicine under the situation that obtains same curative effect after the cell grade micro mist is broken.General medicine can reduce loss of active ingredients in the production link without processing such as lixiviate, decoctions after the cell grade micro mist is broken, maximally utilised raw material, so this technology is suitable for the pulverizing of rare Chinese medicine; And, be convenient to take because the increase of fineness can improve mouthfeel.On the other hand, be in totally-enclosed dustless system, to carry out because of the cell grade micro mist is broken, can effectively avoid outside contamination, make the microorganism of product and dust content be able to control.
Beneficial effect of the present invention is embodied in:
(1) mainly to have adopted multiple Chinese herbal medicine be that primary raw material prepares feed addictive in the present invention, can improve the boar sexual desire significantly, improves ejaculate volume, sperm concentration, time-to-live and non-return rate, and do not have any harmful effect;
(2) Chinese herbal medicine composite feed additive of the present invention, addition is few in use, and is effective.Problems such as have in the traditional Chinese medicine interpolation process that adding proportion is high, cost is high, drug effect is slow, waste is serious and process technology is improper; And adopt the broken technology of cell grade micro mist that effective ingredient is directly come out; The absorption and the bioavilability of medicine have been improved greatly; Shorten the drug effect time, not only reduce addition but also reduce cost, effect is obvious simultaneously.
The specific embodiment
The barrenwort that uses among the embodiment, cynomorium songaricum, Radix Codonopsis, Radix Angelicae Sinensis and matrimony vine are all available from Anhui Province's Bozhou City letter Li De pharmaceutcal corporation, Ltd.
Embodiment 1
Respectively with barrenwort, cynomorium songaricum, Radix Codonopsis, Radix Angelicae Sinensis and drying of Chinese wolfberry; Take by weighing barrenwort 400 grams, cynomorium songaricum 80 grams, Radix Codonopsis 80 grams, Radix Angelicae Sinensis 80 grams, matrimony vine 80 grams then respectively; Section arrangement then mixes then, and mixed mixture is at first carried out 60 order coarse crushings; It is broken to adopt QYF type fluidized bed air flow crusher to carry out the cell grade micro mist then, and product cut size is 25~40 microns (approximately fineness 350~500 orders); To the vitamin A micro-capsule that in the fine mixture of cell grade, adds 500,000 IU/ gram 10 grams, the vitamin D of 500,000 IU/ gram 3Micro-capsule 2 grams, 50% vitamin E powder, 40 grams, 80% Cobastab 2Micro-capsule 3 gram and carrier zeolite powders (100 order fineness) make total amount reach 1000 grams, mix once more, obtain the Chinese herbal medicine composite feed additive.
Embodiment 2
Identical with embodiment 1 method, the weight of the raw material of adding is respectively: barrenwort 500 grams, cynomorium songaricum 100 grams, Radix Codonopsis 80 grams, Radix Angelicae Sinensis 80 grams, matrimony vine 80 grams, vitamin A micro-capsule 10 grams of 500,000 IU/ gram, the vitamin D of 500,000 IU/ gram 3Micro-capsule 2 grams, 50% vitamin E powder, 40 grams, 80% Cobastab 2Micro-capsule 3 gram and carrier zeolite powders (100 order fineness) make total amount reach 1000 grams, according to preparing the Chinese herbal medicine composite feed additive with embodiment 1 identical preparation method.
Embodiment 3
Identical with the method for embodiment 1, the weight of the raw material of adding is respectively: barrenwort 400 grams, cynomorium songaricum 80 grams, Radix Codonopsis 100 grams, Radix Angelicae Sinensis 100 grams and matrimony vine 100 grams, vitamin A micro-capsule 10 grams of 500,000 IU/ gram, the vitamin D of 500,000 IU/ gram 3Micro-capsule 2 grams, 50% vitamin E powder, 40 grams, 80% Cobastab 2Micro-capsule 3 gram and carrier zeolite powders (100 order fineness) make total amount reach 1000 grams, according to preparing the Chinese herbal medicine composite feed additive with embodiment 1 identical preparation method.
Application examples 1
Test place: the just flat plant in Xiaoshan, Hangzhou.
Test period: on June 10th, 2008 beginning, June 30 in 2008 end of day, 20 days experimental periods.
The selection of test pig: select grow up 18 of herd boars of the healthy and strong Du Luoke of physique, first day on-test, taked seminal fluid, measured many indexs such as ejaculate volume, sperm concentration, sperm motility rate, rate of teratosperm, perforatorium percentage of head rice respectively with hand grip, and considered multinomial factor such as boar age; Adjustment and decision are divided into groups; Make each group when the experiment beginning, each item index difference is not remarkable, is divided into 2 processed group to 18 boars; Each processed group is established 3 repetitions, and each repeats 3.
Basal diet prescription and nutrient content: as shown in table 1.
The test daily ration: the additive that utilizes embodiment 1 to prepare is that 0.1% consumption substitutes 0.1% zeolite powder in the basal ration prescription as the test daily ration according to percentage by weight.
Table 1
Figure BSA00000150349300061
Annotate: contain in every kilogram of basal diet: the rope A 10000IU that supports one's family, vitamin D 1500IU, 25 milligrams of vitamin Es, vitamin K 32 milligrams, Cobastab 12 milligrams, Cobastab 24 milligrams, Cobastab 120.025 milligram, 0.2 milligram of biotin, 4 milligrams in folic acid, 25 milligrams in pantothenic acid, 50 milligrams in nicotinic acid; 5 milligrams of Cu, 75 milligrams of Zn, 80 milligrams of Fe, 10 milligrams of Mn, 0.25 milligram of Se, 0.3 milligram of I, 500 milligrams in choline, 300 milligrams in 15% aureomycin; Wherein DE, Lys, Met+Cys and Thr are calculated value, and all the other are measured value; Wherein, the additive that compound premix is made up of trace element and amino acid etc. can adopt commercially available product.
Sample is gathered: in off-test last day, the pig of participating in the experiment use the hand grip collecting semen, and the evaluation semen quality.Duration of test, boar are participated in daily breeding task as usual, and it is basic identical that each organizes usage frequency.
Ejaculate volume test: heavy with electronic scale weighing seminal fluid, by the 1mL/g seminal fluid heavy=(the smart cup of seminal fluid weight+collection is heavy)-collection essence glass weighs.
The sperm concentration test: measuring sperm concentration with the tally counting method, is unit with hundred million/mL.
Sperm motility rate method of testing: adopt 0~1.0 ten grade of point system investigation sperm motility rate; The sperm count that promptly straight line moves ahead in field of view accounts for the percentage of whole sperm counts; The sperm that non-rectilinear moves ahead is not counted eupyrene sperm, the sperm as 10% be straight line move ahead be chosen as 0.1 grade, the rest may be inferred.
The rate of teratosperm assay method: with the abnormal rate of Yihong methylene blue coloring agent dyeing mensuration sperm, the sperm of head, afterbody and kytoplasm deformity is counted defective sperm.
Perforatorium percentage of head rice assay method: adopt Ji's nurse Sa decoration method to measure the perforatorium percentage of head rice, it is that acrosome is complete that acrosome is dyed blue person, and not painted or painted irregular person is that acrosome is damaged.
Data processing method: adopt SPSS16.0 that data is handled, and carry out one-way analysis of variance and least significant difference method (LSD) check.Data are with average ± standard deviation (X ± SD) expression.
Test result: test after 20 days, observe through behaviouristics and find that control group boar sexual desire is poor, mounting is few, and indivedual boar walkings are unable, slow in reacting, are off one's feed.The test group boar state of mind is good, and sexual desire is very vigorous, often climbs on circle door or circle wall and bickers, and spits out white foams, and mounting sow ability is strong, and hair color is bright and clean.Add Chinese herbal medicine composite feed additive of the present invention to boar semen amount, sperm concentration and sperm motility rate to influence test result as shown in table 2.
Table 2
Figure BSA00000150349300071
Annotate: same column shoulder motes lowercase difference person significant difference (p<0.05), capitalization difference person difference is (p<0.01) extremely significantly.
Can know that by table 2 behind the interpolation Chinese herbal medicine composite feed additive of the present invention, test group boar semen amount has improved 15.91% (p<0.01) than control group in the boar feed; Sperm concentration has improved 10.09% (p<0.05) than control group; Sperm motility rate has improved 4.61% (p<0.01) than control group.
Add Chinese herbal medicine composite feed additive of the present invention to boar rate of teratosperm and acrosomal integrity to influence test result as shown in table 3.
Table 3
Figure BSA00000150349300081
Annotate: same column shoulder motes lowercase difference person significant difference (p<0.05), capitalization difference person difference is (p<0.01) extremely significantly.
Can know that by table 3 behind the interpolation Chinese herbal medicine composite feed additive of the present invention, test group boar rate of teratosperm has reduced by 6.08% (p<0.01) than control group in the boar feed; Acrosomal integrity has improved 1.87% (p<0.01) than control group.
Have above-mentioned test result to know, behind the interpolation Chinese herbal medicine composite feed additive of the present invention, the test group boar state of mind is good, is highly sexed in the boar daily ration, and mounting sow ability is strong.In addition, compare with control group, the boar semen amount has improved 15.91% (p<0.01); Sperm concentration has improved 10.09% (p<0.05); Sperm motility rate has improved 4.61% (p<0.01); Rate of teratosperm has reduced by 6.08% (p<0.01); Acrosomal integrity has improved 1.87% (p<0.01).Show that adding Chinese herbal medicine composite feed additive of the present invention has obvious effects to improving boar sexual desire and fecundity.
Application examples 2
Test period: on August 10th, 2008 beginning, Augusts 30 in 2008 end of day, 20 days experimental periods.
Test place: with application examples 1.
The selection of test pig: select grow up 18 of herd boars of the healthy and strong Du Luoke of physique, first day on-test, taked seminal fluid, measured many indexs such as ejaculate volume, sperm concentration, sperm motility rate, rate of teratosperm, perforatorium percentage of head rice respectively with hand grip, and considered multinomial factor such as boar age; Adjustment and decision are divided into groups; Make each group when the experiment beginning, each item index difference is not remarkable, is divided into 2 processed group to 18 boars; Each processed group is established 3 repetitions, and each repeats 3.
The test daily ration: the additive that utilizes embodiment 2 to prepare is that 0.1% consumption substitutes 0.1% zeolite powder in the basal ration prescription as the test daily ration according to percentage by weight.
Basal diet, process of the test processing and data acquisition and data processing method are with application examples 1.
Test result: test after 20 days, observe through behaviouristics and find that control group boar sexual desire is poor, mounting is few, and indivedual boar walkings are unable, slow in reacting, are off one's feed.The test group boar state of mind is good, and sexual desire is very vigorous, often climbs on circle door or circle wall and bickers, and spits out white foams, and mounting sow ability is strong, and hair color is bright and clean.To add Chinese herbal medicine composite feed additive of the present invention to boar semen amount, sperm concentration and sperm motility rate to influence test result as shown in table 4.
Table 4
Figure BSA00000150349300091
Annotate: same column shoulder motes lowercase difference person significant difference (p<0.05), capitalization difference person difference is (p<0.01) extremely significantly.
Can know that by table 4 behind the interpolation Chinese herbal medicine composite feed additive of the present invention, test group boar semen amount has improved 14.05% (p<0.01) than control group in the boar feed; Sperm concentration has improved 11.48% (p<0.01) than control group; Sperm motility rate has improved 4.97% (p<0.01) than control group.
Add Chinese herbal medicine composite feed additive of the present invention to boar rate of teratosperm and acrosomal integrity to influence test result as shown in table 5.
Table 5
Annotate: same column shoulder motes lowercase difference person significant difference (p<0.05), capitalization difference person difference is (p<0.01) extremely significantly.
Can know that by table 5 behind the interpolation Chinese herbal medicine composite feed additive of the present invention, test group boar rate of teratosperm has reduced by 4.83% (p<0.05) than control group in the boar feed; Acrosomal integrity has improved 1.68% (p<0.01) than control group.
Have above-mentioned test result to know, behind the interpolation Chinese herbal medicine composite feed additive of the present invention, the test group boar state of mind is good, is highly sexed in the boar daily ration, and mounting sow ability is strong.In addition, compare with control group, the boar semen amount has improved 14.05% (p<0.01); Sperm concentration has improved 11.48% (p<0.01); Sperm motility rate has improved 4.97% (p<0.01); Rate of teratosperm has reduced by 4.83% (p<0.05); Acrosomal integrity has improved 1.68% (p<0.01).Show that adding Chinese herbal medicine composite feed additive of the present invention has obvious effects to improving boar sexual desire and fecundity.

Claims (2)

1. feed addictive that improves reproductive capacity of boar, the percentage by weight of its raw material consists of:
Figure FDA0000130727380000011
The preparation method of described feed addictive comprises: with percentage by weight is that 40~50% barrenwort, 8~10% cynomorium songaricum, 8~10% Radix Codonopsis, 8~10% Radix Angelicae Sinensis and 8~10% matrimony vine mix, and being crushed to particle diameter is 25~40 microns; Adding percentage by weight is 0.5~1% vitamin A, 0.1~0.2% vitamin D 3, 1~4% vitamin E, 0.1~0.3% Cobastab 2And zeolite powder, mix.
2. feed addictive according to claim 1 is characterized in that, the percentage by weight of described raw material consists of:
Figure FDA0000130727380000012
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CN1989831A (en) * 2005-12-28 2007-07-04 上海创博生态工程有限公司 Microorganism feed addictive improving reproduction ability of boar and making method thereof
CN1876011A (en) * 2006-04-04 2006-12-13 华中农业大学 A pharmaceutical composition capable of improving boar reproductive performance
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