CN1843456A - Method for extracting polysaccharide from Chinese formula of 'Yi Guan Jian' - Google Patents
Method for extracting polysaccharide from Chinese formula of 'Yi Guan Jian' Download PDFInfo
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- CN1843456A CN1843456A CN 200510063406 CN200510063406A CN1843456A CN 1843456 A CN1843456 A CN 1843456A CN 200510063406 CN200510063406 CN 200510063406 CN 200510063406 A CN200510063406 A CN 200510063406A CN 1843456 A CN1843456 A CN 1843456A
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- yiguanjian
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Abstract
The invention discloses a novel process for extracting Yiguanjian polysaccharide from Yiguanjian Decoction and the use of Yiguanjian polysaccharide in the treatment of acute and chronic hepatitis. The method employs the steps of water extraction, alcohol depositing and refining.
Description
Technical field
The present invention relates to the Chinese drug preparation technique field, disclose a kind of method of from raw material of Chinese medicine such as Radix Glehniae, Radix Ophiopogonis, Radix Angelicae Sinensis, extracting the YIGUANJIAN polysaccharide.
Background technology
Chinese medicinal formulae " YIGUANJIAN " comes from " Xu Mingyi Lei " of the well-known doctor Wei Zhi Xian of the Qing Dynasty, is the first-selected prescription of nourishing YIN and dispersing the stagnated liver-QI clinically.In recent years, Chinese scholars confirms that the effect of YIGUANJIAN nourishing YIN and dispersing the stagnated liver-QI is outstanding really, and is widely used in all diseases of the hepatic and renal YIN deficiency such as liver, stomach, hypertension, gynecological, ophthalmology by a large amount of pharmacology and clinical research.
YIGUANJIAN is usually used in clinical medicament and adopts traditional preparation process technology more, is YIGUANJIAN water crude extract.The present invention adopts decoction and alcohol sedimentation technique in conjunction with pharmacological research, has invented a kind of new method of extracting the YIGUANJIAN polysaccharide from YIGUANJIAN, and it is simple to have extraction process, active constituent content height, characteristics such as steady quality and easy control.
Summary of the invention
The technical problem to be solved in the present invention be disclose a kind of from Chinese medicinal formulae " YIGUANJIAN " method of effective component extracting YIGUANJIAN polysaccharide.
The present invention is achieved in that
(1) get a certain amount of Radix Glehniae, Radix Ophiopogonis, Radix Angelicae Sinensis, the Radix Rehmanniae, Fructus Lycii, Fructus Toosendan, add 4-10 and doubly measure decoction 1-4 hour, coarse filtration is collected decoction liquor;
(2) decoction liquor being concentrated, is the extractum of 1.0-1.5 to density;
(3) in extractum, add ethanol, leave standstill, filter, collect filtrate;
(4) in filtrate, add ethanol, make solution contain the alcohol amount and reach 30%-90%, leave standstill, centrifugal, and the collecting precipitation thing;
(5) precipitate is dissolved in water, uses the 0.1%-10% activated carbon decolorizing, filter, get medicinal liquid.
A kind of method of extracting the YIGUANJIAN polysaccharide of the present invention is characterized in that the YIGUANJIAN water cooking liquid is condensed into the extractum that density is 1.0-1.5; And use dissolve with ethanol extractum, and make solution contain the alcohol amount and reach 30-90%, leave standstill, centrifugal, taking precipitate; After being dissolved in water, use the 0.1%-10% activated carbon decolorizing, filter, get medicinal liquid.
The specific embodiment
Embodiment:
(1) get a certain amount of Radix Glehniae, Radix Ophiopogonis, Radix Angelicae Sinensis, the Radix Rehmanniae, Fructus Lycii, Fructus Toosendan, add 8 times of amounts and decocted 2 hours, coarse filtration is collected decoction liquor;
(2) decoction liquor being concentrated, is the extractum of 1.1-1.5 to density;
(3) in extractum, add 95% ethanol, leave standstill, filter, collect filtrate;
(4) in filtrate, add 95% ethanol, make solution contain the alcohol amount and reach 80%, leave standstill, centrifugal, and the collecting precipitation thing;
(5) precipitate is dissolved in water, uses the 0.1%-0.5% activated carbon decolorizing, filter, get medicinal liquid.
Show that through pharmacological experiment study the YIGUANJIAN polysaccharide that extracts by the present invention can improve body's immunity, strengthen the activity of rat phagocyte; Can reduce acute and chronic liver injury rat model serum alt due to the CCL4, AST, obviously alleviate hepatic tissue pathology and sexually revise regulating liver-QI hamartoplasia; Can promote liver cell regeneration and repair, suppress the hypertrophy of collagen fiber; Show that the YIGUANJIAN polysaccharide that the present invention extracts has unique pharmacological action to various acute, chronic hepatitis.Therefore, the application of YIGUANJIAN polysaccharide in the acute, chronic hepatitis field of the present invention's extraction also belongs to protection domain of the present invention.Wherein acute, chronic hepatitis is meant viral and acute icterohepatitis, acute anicteric hepatitis, chronic hepatitis, chronic active hepatitis, cholestatic hepatitis, hepatitis gravis (acute, subacute, chronic heavy type) non-viral.
The YIGUANJIAN polysaccharide of extraction of the present invention also can be mixed with various dosage forms according to the clinical practice needs, as injection (injection, powder ampoule agent for injection, infusion solutions), pill, powder, tablet, suppository, granule, membrane, capsule, drop pill, aerosol, microcapsule, unguentum, medicated wine, syrup, oral solution.
Claims (5)
1. a method of extracting the YIGUANJIAN polysaccharide is characterized in that, has adopted the method for re-refining behind the water extract-alcohol precipitation.
2. a kind of method of extracting the YIGUANJIAN polysaccharide according to claim 1 is characterized in that its extracting method comprises following steps:
(1) get a certain amount of Radix Glehniae, Radix Ophiopogonis, Radix Angelicae Sinensis, the Radix Rehmanniae, Fructus Lycii, Fructus Toosendan, add 4-10 and doubly measure decoction 1-4 hour, coarse filtration is collected decoction liquor;
(2) decoction liquor being concentrated, is the extractum of 1.0-1.5 to density;
(3) in extractum, add ethanol, leave standstill, filter, collect filtrate;
(4) in filtrate, add ethanol, make solution contain the alcohol amount and reach 30%-90%, leave standstill, centrifugal, and the collecting precipitation thing;
(5) precipitate is dissolved in water, uses the 0.1%-10% activated carbon decolorizing, filter, get medicinal liquid.
3. a kind of method of extracting the YIGUANJIAN polysaccharide according to claim 1 and 2 is characterized in that, the YIGUANJIAN water cooking liquid is condensed into the extractum that density is 1.0-1.5; And use dissolve with ethanol extractum, and make solution contain the alcohol amount and reach 30-90%, leave standstill, centrifugal, taking precipitate; After being dissolved in water, use the 0.1%-10% activated carbon decolorizing, filter, get medicinal liquid.
4, according to claim 1 or 2 or 3 described a kind of methods of extracting the YIGUANJIAN polysaccharide, it is characterized in that, application in the acute, chronic hepatitis treatment, wherein acute, chronic hepatitis is meant viral and acute icterohepatitis, acute anicteric hepatitis, chronic hepatitis, chronic active hepatitis, cholestatic hepatitis, hepatitis gravis (acute, subacute, chronic heavy type) non-viral.
5. according to claim 1 or 2 or 3 or 4 described a kind of methods of extracting the YIGUANJIAN polysaccharide, it is characterized in that, the YIGUANJIAN polysaccharide that extracts also can be mixed with various dosage forms according to the clinical practice needs, as injection (injection, powder ampoule agent for injection, infusion solutions), pill, powder, tablet, suppository, granule, membrane, capsule, drop pill, aerosol, microcapsule, unguentum, medicated wine, syrup, oral solution.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200510063406 CN1843456A (en) | 2005-04-07 | 2005-04-07 | Method for extracting polysaccharide from Chinese formula of 'Yi Guan Jian' |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200510063406 CN1843456A (en) | 2005-04-07 | 2005-04-07 | Method for extracting polysaccharide from Chinese formula of 'Yi Guan Jian' |
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| CN1843456A true CN1843456A (en) | 2006-10-11 |
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| CN 200510063406 Pending CN1843456A (en) | 2005-04-07 | 2005-04-07 | Method for extracting polysaccharide from Chinese formula of 'Yi Guan Jian' |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104383198A (en) * | 2014-12-11 | 2015-03-04 | 丁铁梅 | Traditional Chinese medicine for treating chronic hepatitis |
| CN104383199A (en) * | 2014-12-11 | 2015-03-04 | 丁铁梅 | Traditional Chinese medicine used for treating chronic hepatitis |
| CN112190655A (en) * | 2020-11-13 | 2021-01-08 | 上海中医药大学附属曙光医院 | Traditional Chinese medicine composition for treating hepatic sinus obstruction syndrome and application thereof |
| CN114732868A (en) * | 2021-12-13 | 2022-07-12 | 湖南药圣堂中药科技有限公司 | Preparation method of standard decoction for consistent decoction |
| CN116942784A (en) * | 2023-08-11 | 2023-10-27 | 广州市普森生物科技有限公司 | Exosome preparation for relieving liver injury and preparation method and application thereof |
-
2005
- 2005-04-07 CN CN 200510063406 patent/CN1843456A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104383198A (en) * | 2014-12-11 | 2015-03-04 | 丁铁梅 | Traditional Chinese medicine for treating chronic hepatitis |
| CN104383199A (en) * | 2014-12-11 | 2015-03-04 | 丁铁梅 | Traditional Chinese medicine used for treating chronic hepatitis |
| CN112190655A (en) * | 2020-11-13 | 2021-01-08 | 上海中医药大学附属曙光医院 | Traditional Chinese medicine composition for treating hepatic sinus obstruction syndrome and application thereof |
| CN114732868A (en) * | 2021-12-13 | 2022-07-12 | 湖南药圣堂中药科技有限公司 | Preparation method of standard decoction for consistent decoction |
| CN116942784A (en) * | 2023-08-11 | 2023-10-27 | 广州市普森生物科技有限公司 | Exosome preparation for relieving liver injury and preparation method and application thereof |
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