CN103800784A - Fructus alpiniae oxyphyllae and compatible lindera aggregata extract for treating diabetes, and preparation method thereof - Google Patents
Fructus alpiniae oxyphyllae and compatible lindera aggregata extract for treating diabetes, and preparation method thereof Download PDFInfo
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- CN103800784A CN103800784A CN201410048228.8A CN201410048228A CN103800784A CN 103800784 A CN103800784 A CN 103800784A CN 201410048228 A CN201410048228 A CN 201410048228A CN 103800784 A CN103800784 A CN 103800784A
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Abstract
The invention discloses a fructus alpiniae oxyphyllae and compatible lindera aggregata extract for treating diabetes. The extract mainly comprises extract of petroleum ether part of fructus alpiniae oxyphyllae and compatible lindera aggregata extract, wherein the fructus alpiniae oxyphyllae and lindera aggregata are decocted with water according to the proportion of 1: 1 in a conventional way for three times, and concentrated solution is collected, so that dry extract can be obtained. The method comprises the steps of grinding, screening, carrying out reflux extraction, filtering, adding a solvent for extracting, recovering the solvent, carrying out ultrasonic extraction on lindera aggregata extract solution and preparing. According to the fructus alpiniae oxyphyllae and compatible lindera aggregata extract for treating the diabetes, and a preparation method of the extract provided by the invention, the harm of the diabetes to a patient is improved by the petroleum ether part of fructus alpiniae oxyphyllae extract, diabetic nephropathy (DN) can be treated by the extract, and kidney function decrease can be delayed; the fructus alpiniae oxyphyllae and compatible lindera aggregata extract is capable of promoting down regulation of the p27kip1 protein expression, has the functions of relieving kidney hypertrophia, reducing proteinuria and protecting the functions of the kidney, can effectively prevent diabetes complications, and is good in diabetes treatment effect.
Description
Technical field
The technical field that the invention belongs to the plant extract for the treatment of nephropathy, relates in particular to a kind of Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes and preparation method thereof.
Background technology
Diabetes spp is in the Chinese medicine category of " quenching one's thirst ", be impairment of the kidney is mainly the development trend of DN, diabetic nephropathy (DN) was mainly divided into for 5 phases clinically, relatively concealment of front 3 phases morbidity, once enter for the 4th phase, there is dominant albumen, indicating that the state of an illness is very serious, and still lacking at present effective treatment means.Expression of Cyclin Kinase Inhibitor P27 increases, in diabetes (DM) messangial cell hypertrophy, play an important role, diabetic nephropathy (diabetic nephropathy, DN) be one of topmost vascular complication of diabetes, also be the major reason that diabetics is lethal, most of end-stage renal failure patient comes from DN.Along with China's growth in the living standard, the sickness rate of diabetes also rises year by year, and how effectively preventing DN becomes day by day urgent.For many years, DN has been carried out to large quantity research both at home and abroad, but because of its pathogenetic complexity, the existing treatment measure of DN can only partly delay the process of DN, cannot fundamentally block the progress of DN.If the associated treatment drug main Western medicine using at present, and be mainly symptomatic treatment, the patient who has complication for some, has just formed certain limitation, can not effectively solve the progress of DN.At present, plant extract and the method thereof of traditional treatment diabetes have some limitations, the problem of complication prevention and treatment diabetes weak effect.
Summary of the invention
The object of the embodiment of the present invention is to provide a kind of Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes and preparation method thereof, and plant extract and the method thereof that is intended to solve traditional treatment diabetes has some limitations, the problem of complication prevention and treatment diabetes weak effect.
The embodiment of the present invention is achieved in that a kind of Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes, and Fructus Alpiniae Oxyphyllae, the Radix Linderae carry out three collection concentrated solutions of conventional decocting in 1: 1 ratio and obtain dry extract; This extract mainly comprises Fructus Alpiniae Oxyphyllae petroleum ether part extract, compatibility Radix Linderae extract.
Further, the Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract of described treatment diabetes, to promoting p27kip1 protein expression to lower, plays certain preventive and therapeutic effect to diabetics.
A kind of Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes and preparation method thereof, the method comprises pulverizing, screening, reflux, extract,, filters, adds solvent extraction, reclaims solvent, supersound extraction Radix Linderae extracting solution, preparation;
Described pulverizing refers to gets Fructus Alpiniae Oxyphyllae 100g, utilizes pulverizer to carry out processed powdered;
Described screening refers to and uses sieves to screen powder, the powder sifting out is placed in the round-bottomed flask of 2000ml;
Described reflux, extract, refers to and in powder solid, adds ethanol 600ml reflux, extract, twice, and each 1h filters with gauze;
Described filtration refers to and merges the solution that twice filtration obtains, decompression and solvent recovery below 60 ℃, and it is even that extractum adds suitable quantity of water suspendible, is dispersed to 100ml;
Described add solvent extraction to refer in solution, to use successively petroleum ether (60-90 ℃), chloroform, ethyl acetate, n-butanol extraction, and separate each layer;
Described recovery solvent refers to the solution of each layer is reduced pressure respectively and reclaims solvent, obtains the extract at each position, takes out the solute of petroleum ether part as medication, is mixed with containing the stable suspension of 5%Twen-80 for subsequent use;
Described supersound extraction Radix Linderae extracting solution refers to and utilizes ultrasound wave to extract Radix Linderae volatile oil to Combined Spicebush Root;
Described preparation refers to the extract of Fructus Alpiniae Oxyphyllae petroleum ether part and Radix Linderae volatile oil mixed, and the preparation that stirs generates the Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes, is prepared into that to amount to the medicinal liquid of 1g crude drug/ml for subsequent use.
Further, the Fructus Alpiniae Oxyphyllae in described pulverizing is specifically purchased in Haikou City, Hainan Province Guo Ruitang pharmaceuticals.
Further, the Fructus Alpiniae Oxyphyllae petroleum ether part extract in described recovery solvent can, by suppressing the expression of AQP2, have the effect of good inhibition urinary protein excretion, realizes the effect of protection kidney merit.
Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes provided by the invention and preparation method thereof, utilizes Fructus Alpinae Oxyphyllae extract petroleum ether part to improve the infringement of patient's diabetes, and treatment DN, delays renal hypofunction; Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract promotes p27kip1 protein expression to lower, and has the renal hypertrophy of alleviating degree, alleviates albuminuria, and the function of protection kidney, effectively prevents glycosuria disease complication, and treatment diabetes are effective.
Accompanying drawing explanation
Fig. 1 is the Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract of the treatment diabetes that provide of the embodiment of the present invention and preparation method thereof flow chart of steps.
The expression (western blotting) (1,2,3: model group of Fig. 2 .DN mice P27kip1 under different situations; 4,5: irbesartan group; 6,7: Fructus Alpiniae Oxyphyllae extract group; 10: Normal group) 8,9: Fructus Alpiniae Oxyphyllae-Radix Linderae medicine is to extract group.
The specific embodiment
In order to make object of the present invention, technical scheme and advantage clearer, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein, only in order to explain the present invention, is not intended to limit the present invention.
Below in conjunction with drawings and the specific embodiments, application principle of the present invention is further described.
As shown in Figure 1, a kind of Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes, this extract mainly comprises Fructus Alpiniae Oxyphyllae petroleum ether part extract, compatibility Radix Linderae extract.
Further, the Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract of described treatment diabetes, to promoting p27kip1 protein expression to lower, plays certain preventive and therapeutic effect to diabetics.
A kind of Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes and preparation method thereof, the method comprises pulverizing S101, screening S102, reflux, extract, S103, filters S104, adds solvent extraction S105, reclaims solvent S106, supersound extraction Radix Linderae extracting solution S107, prepares S108;
Described pulverizing S101 refers to and gets Fructus Alpiniae Oxyphyllae 100g, utilizes pulverizer to carry out processed powdered;
Described screening S102 refers to and uses sieves to screen powder, the powder sifting out is placed in the round-bottomed flask of 2000ml;
Described reflux, extract, S103 refers to and in powder solid, adds ethanol 600ml reflux, extract, twice, and each 1h filters with gauze;
Described filtration S104 refers to and merges the solution that twice filtration obtains, decompression and solvent recovery below 60 ℃, and it is even that extractum adds suitable quantity of water suspendible, is dispersed to 100ml;
Described add solvent extraction S105 to refer in solution, to use successively petroleum ether (60-90 ℃), chloroform, ethyl acetate, n-butanol extraction, and separate each layer;
Described recovery solvent S106 refers to the solution of each layer is reduced pressure respectively and reclaims solvent, obtains the extract at each position, takes out the solute of petroleum ether part as medication, is mixed with containing the stable suspension of 5%Twen-80 for subsequent use;
Described supersound extraction Radix Linderae extracting solution S107 refers to and utilizes ultrasound wave to extract Radix Linderae volatile oil to Combined Spicebush Root;
Described preparation S108 refers to the extract of Fructus Alpiniae Oxyphyllae petroleum ether part and Radix Linderae volatile oil mixed, and the preparation that stirs generates the Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes, is prepared into that to amount to the medicinal liquid of 1g crude drug/ml for subsequent use.
Further, the Fructus Alpiniae Oxyphyllae in described pulverizing S101 is specifically purchased in Haikou City, Hainan Province Guo Ruitang pharmaceuticals.
Further, the Fructus Alpiniae Oxyphyllae petroleum ether part extract in described recovery solvent S106 can, by suppressing the expression of AQP2, have the effect of good inhibition urinary protein excretion, realizes the effect of protection kidney merit.
Operation principle
As shown in Figure 1, a kind of Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes and preparation method thereof comprises pulverizing S101, screening S102, reflux, extract, S103, filters S104, adds solvent extraction S105, reclaims solvent S106, supersound extraction Radix Linderae extracting solution S107, prepares S108; Described pulverizing S101 refers to and gets Fructus Alpiniae Oxyphyllae 100g, utilizes pulverizer to carry out processed powdered; Described screening S102 refers to and uses sieves to screen powder, the powder sifting out is placed in the round-bottomed flask of 2000ml; Described reflux, extract, S103 refers to and in powder solid, adds ethanol 600ml reflux, extract, twice, and each 1h filters with gauze; Described filtration S104 refers to and merges the solution that twice filtration obtains, decompression and solvent recovery below 60 ℃, and it is even that extractum adds suitable quantity of water suspendible, is dispersed to 100ml; Described add solvent extraction S105 to refer in solution, to use successively petroleum ether (60-90 ℃), chloroform, ethyl acetate, n-butanol extraction, and separate each layer; Described recovery solvent S106 refers to the solution of each layer is reduced pressure respectively and reclaims solvent, obtains the extract at each position, takes out the solute of petroleum ether part as medication, is mixed with containing the stable suspension of 5%Twen-80 for subsequent use; Described supersound extraction Radix Linderae extracting solution S107 refers to and utilizes ultrasound wave to extract Radix Linderae volatile oil to Combined Spicebush Root; Described preparation S108 refers to the extract of Fructus Alpiniae Oxyphyllae petroleum ether part and Radix Linderae volatile oil mixed, and the preparation that stirs generates the Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes, is prepared into that to amount to the medicinal liquid of 1g crude drug/ml for subsequent use.
The comparison of each group mice fasting glucose
With Normal group comparison, all the other are respectively organized mouse blood sugar and significantly raise (P < 0.01), and there was no significant difference (P > 0.05) between these three groups.Along with time lengthening, each medicine group blood glucose there is no obvious reduction, with model group zero difference (P > 0.05), in table 1.
Note: with Normal group comparison, respectively organize blood glucose significantly raise (* P < 0.01); With model group comparison, each intervention group blood glucose is without obviously reducing (#P > 0.05).
Each group mice twenty-four-hour urine albumen situation
Compared with Normal group, after the twenty-four-hour urine protein quantification administration of model group, obviously raise (P < 0.01); Compared with model group, the value of each administration group obviously reduces (P < 0.01), in table 2.
Note: with model group comparison, * P < 0.01.With irbesartan comparison, (#P > 0.05).
Western blott ing result
With Normal group comparison, the glomerule P27kip1 expression of DN model group obviously increases; With model group comparison, the P27kip1 expression of pharmaceutical intervention group obviously declines, and irbesartan group and Fructus Alpiniae Oxyphyllae extract group are without obvious difference.In table 3, Fig. 2.
Note: with model group comparison, * P < 0.01.With irbesartan comparison, squeak P > 0. (ix)).
RT-PCR result
With Normal group comparison, the Mus p27Kip1 gene expression dose of DN model group obviously increases; With model group comparison, Mus p27Kip1 gene expression dose obviously declines, and irbesartan group and Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract (petroleum ether part) group are without obviously difference.In table 4.
Note: with model group comparison, * P < 0.01.With irbesartan comparison, (#P > 0.05).
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.
Claims (3)
1. treat the Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract of diabetes for one kind, it is characterized in that, this extract mainly comprises Fructus Alpiniae Oxyphyllae petroleum ether part extract, compatibility Radix Linderae extract, and Fructus Alpiniae Oxyphyllae, the Radix Linderae carry out three collection concentrated solutions of conventional decocting in 1: 1 ratio and obtain dry extract.
2. the Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes as claimed in claim 1, is characterized in that, the Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract of described treatment diabetes is to promoting p27kip1 protein expression to lower.
3. treat Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract of diabetes and preparation method thereof for one kind, it is characterized in that, the method comprises pulverizing, screening, reflux, extract,, filters, adds solvent extraction, reclaims solvent, supersound extraction Radix Linderae extracting solution, preparation;
Described pulverizing refers to gets Fructus Alpiniae Oxyphyllae 100g, utilizes pulverizer to carry out processed powdered;
Described screening refers to and uses sieves to screen powder, the powder sifting out is placed in the round-bottomed flask of 2000ml;
Described reflux, extract, refers to and in powder solid, adds ethanol 600ml reflux, extract, twice, and each 1h filters with gauze;
Described filtration refers to and merges the solution that twice filtration obtains, decompression and solvent recovery below 60 ℃, and it is even that extractum adds suitable quantity of water suspendible, is dispersed to 100ml;
Described add solvent extraction to refer in solution, to use successively petroleum ether, chloroform, ethyl acetate, n-butanol extraction, and separate each layer;
Described recovery solvent refers to the solution of each layer is reduced pressure respectively and reclaims solvent, obtains the extract at each position, takes out the solute of petroleum ether part as medication, is mixed with containing the stable suspension of 5%Twen-80 for subsequent use;
Described supersound extraction Radix Linderae extracting solution refers to and utilizes ultrasound wave to extract Radix Linderae volatile oil to Combined Spicebush Root;
Described preparation refers to the extract of Fructus Alpiniae Oxyphyllae petroleum ether part and Radix Linderae volatile oil mixed, and the preparation that stirs generates the Fructus Alpiniae Oxyphyllae compatibility Radix Linderae extract for the treatment of diabetes, is prepared into that to amount to the medicinal liquid of 1g crude drug/ml for subsequent use.
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CN105012826A (en) * | 2015-07-20 | 2015-11-04 | 河南大学 | Alpinia oxyphylla leaf extract and preparation method and application thereof |
CN105878894A (en) * | 2016-06-15 | 2016-08-24 | 海南医学院 | Traditional Chinese medicine composition used for treating diabetic nephropathy, traditional Chinese medicine preparation and preparation method of preparation |
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Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN103861003A (en) * | 2014-03-18 | 2014-06-18 | 海南医学院 | Alpinia oxyphylla-radix lindera extract for treating nephropathy and preparation method of extract |
CN105012826A (en) * | 2015-07-20 | 2015-11-04 | 河南大学 | Alpinia oxyphylla leaf extract and preparation method and application thereof |
CN105012826B (en) * | 2015-07-20 | 2018-09-21 | 河南大学 | A kind of intelligence development leaf extract and preparation method thereof, application |
CN105878894A (en) * | 2016-06-15 | 2016-08-24 | 海南医学院 | Traditional Chinese medicine composition used for treating diabetic nephropathy, traditional Chinese medicine preparation and preparation method of preparation |
CN105878894B (en) * | 2016-06-15 | 2017-05-31 | 海南医学院 | A kind of Chinese medicine composition, Chinese medicine preparation for treating diabetic nephropathy and preparation method thereof |
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