CN1823569A - Cultivation of selenium enriched morin and method of extracting morin selenium polysacharide - Google Patents
Cultivation of selenium enriched morin and method of extracting morin selenium polysacharide Download PDFInfo
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- CN1823569A CN1823569A CN 200610045903 CN200610045903A CN1823569A CN 1823569 A CN1823569 A CN 1823569A CN 200610045903 CN200610045903 CN 200610045903 CN 200610045903 A CN200610045903 A CN 200610045903A CN 1823569 A CN1823569 A CN 1823569A
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Abstract
A method for culturing the Se-enriched sporophores of phellinus igniarius and extracting the selenopolyose from them includes such steps as preparing the mother culture medium from 13 raw materials including potato, agar, glucose, bean powder, etc, preparing stock culture medium from 14 raw materials including mulberry wood chip, wheat bran, corn flour, bean powder, etc, inoculating, culturing stock, culturing three-class spawn, preparing mycoplasm, and extracting selenopolyose. It can be used for preventing and treating cancer.
Description
Technical field
The present invention relates to the cultivation method and the application of medicinal fungi, specifically relate to the cultivation of rich selenium Phellinus and the method for extraction Phellinus selenium polysaccharide.
Background technology
Phellinus is the excellent species in the good fungi of the anticancer effect known to the public, and its medical value is recorded in " the wooden careless detailed outline " of Li Shizhen (1518-1593 A.D.) the earliest, and its fruit body costs an arm and a leg, and Chang Yiqi fruit body among the people is used as medicine, to treat multiple eqpidemic disease.Because the natural resources wretched insufficiency of Phellinus, this causes very big breach for the huge market demand, and imbalance between supply and demand is quite outstanding.The artificial cultivation cycle is long, does not form scale as yet, and being difficult to provides required for market.Selenium also has very high medical value, and it is an indispensable element in the human body growth metabolism process, can promote immunologic function, the opposing virus of organism, the prevention variation.But selenium only is converted into biogenic organic selenium by inorganic, just has the assurance of absorption rate height, safety non-toxic, the good medical value of its antitumor grade of competence exertion.Yet do not find product in the market, and the report of selenium-rich phellinus igniarius kind about the combination of bacterium selenium.
Summary of the invention
The purpose of this invention is to provide the combination of a kind of bacterium selenium rich selenium Phellinus cultivation and extract the method for Phellinus selenium polysaccharide.
The cultivation method of rich selenium Phellinus is characterized in that this method comprises the steps:
1) the female preparation of planting of rich selenium Phellinus; The female kind of rich selenium Phellinus is to be made by weight ratio by following compositions: 150～250 parts of potatos, 10～30 parts in agar, glucose 10-30 part, 5～15 parts of bean powderes, 1～3 part in magnesium sulfate, 0.5～1.5 part in zinc sulphate, 1～10 part of potassium dihydrogen phosphate, 1～10 part of peptone, 0.5～1.5 part of tryptophan, 6-BA0.5～1.5 part, 1～5 part of rich selenium fertilizer, mulberry leaves or 50～150 parts of Ramulus Mori, 500～1500 parts in water;
Method for making: potato is cleaned section, and mulberry leaves or Ramulus Mori are cleaned, and mix with other composition, and after mixing, slow fire boils to be endured; With 8 layers of filtered through gauze, filtrate packing test tube is made mother culture media after the agar dissolving; medium was sterilized 20～40 minutes under 110～130 ℃ of temperature, pressure 0.1～0.15Mpa condition; aseptic inoculation Phellinus bacterial strain, 25 ℃ of unglazed cultivations of constant temperature 15 days can obtain described female the kind.
Described Phellinus bacterial strain is a wild Phellinus fruit body of picking up from forest zone, master mountain range, Mudanjiang, Heilongjiang Province.
As stated above the rich selenium Phellinus of gained femalely plant that mycelia is dense, sturdy, color and luster cadmium yellow, selenium polysaccharide content height.
2) preparation of rich selenium Phellinus original seed; Rich selenium Phellinus original seed is to be made by weight ratio by following compositions: 30～40 parts of mulberry tree wood chips, 30～40 parts of hardwood crumbses, 10～20 parts in wheat bran, 2～10 parts of corn flours, 1～10 part of bean powder, 0.5～1.5 part in gypsum, 0.1～1 part in magnesium sulfate, 0.1～1 part in zinc sulphate, 0.05～0.15 part of 6-BA, 0.5～1.5 part in brown sugar, 0.5～1.5 part of lime, 0.1～1 part of potassium dihydrogen phosphate, 0.5～1.5 part of rich selenium fertilizer, 60～70 parts in water, pH value are 7;
Method for making: after the mentioned component mixing and stirring bottling or the pack, seal, under 120～135 ℃ of temperature, pressure 0.1～0.2Mpa condition, sterilized 2 hours; the female kind of the rich selenium Phellinus of aseptic inoculation; 25 ℃ of unglazed cultivation and fermentation of constant temperature 40～50 days, mycelia is covered with, and promptly gets original seed.
Regular, sturdy, the individual weight of gained original seed fruit body.
3) preparation of three grades of kinds; With step 2) pedigree seed culture medium inoculation original seed promptly make three grades of kinds;
4) preparation of sclerotinite; With step 2) three grades of kinds of pedigree seed culture medium inoculation promptly make the selenium-rich phellinus igniarius rod, i.e. sclerotinite or fruit body.
Described rich selenium fertilizer is meant one or more in sodium selenite, sodium selenate or the calcium selenate.
Described 6-BA is an auximone, all is applicable to this cultivating bacterial spawn through this series products of evidence.
Extract the method for Phellinus selenium polysaccharide with prepared selenium-rich phellinus igniarius plastid; it is characterized in that: selenium-rich phellinus igniarius plastid or fruit body powder are broken into the particle that particle diameter is 0.2-0.6mm; use water logging then; be after fully stirring under 70-80 ℃ the condition in temperature, filter, concentrate, drying promptly makes rich selenium Phellinus polysaccharide.
Compared with prior art; advantage of the present invention is: the component of basal culture medium combines the sufficient condition that provides for polysaccharide with selenium; for fungi polysaccharide has been created good environment to the enrichment of selenium; cause sending out of Phellinus and produce strong resistance in bacterium, the reproductive process, reduce the generation of disease, the output of product and quality all are greatly improved; through the test contrast; mycelium is sent out bacterium speed than ordinary culture medium fast 10 days, and pollution rate is low by 10%, and anticancer effectiveness improves more than 30%.Its significant medicinal efficacy of the fungi of being cultivated will further manifest in practice in view of the above, if it is played a role as a kind of Selenium supplement agent safely and effectively or healthcare products, will have more wide prospect.
Embodiment
Embodiment 1:
The cultivation method of rich selenium Phellinus, this method comprises the steps:
1) the female preparation of planting of rich selenium Phellinus; The female kind of rich selenium Phellinus is to be made by weight ratio by following compositions: 200 parts of potatos, 20 parts in agar, 20 parts of glucose, 10 parts of bean powderes, 2 parts in magnesium sulfate, 1 part in zinc sulphate, 5 parts of potassium dihydrogen phosphates, 5 parts of peptones, 1 part of tryptophan, 6-BA1 part, fertile 2 parts of rich selenium, mulberry leaves or 100 parts of Ramulus Mori, 1000 parts in water;
Method for making: potato is cleaned section, and mulberry leaves or Ramulus Mori are cleaned, and mix with other composition, and after mixing, slow fire boils to be endured; With 8 layers of filtered through gauze, filtrate packing test tube is made mother culture media after the agar dissolving, medium was sterilized 20～40 minutes under 110～130 ℃ of temperature, pressure 0.1～0.15Mpa condition, aseptic inoculation Phellinus bacterial strain, 25 ℃ of unglazed cultivations of constant temperature 15 days can obtain described female the kind;
2) preparation of rich selenium Phellinus original seed; Rich selenium Phellinus original seed is to be made by weight ratio by following compositions: 35 parts of mulberry tree wood chips, 35 parts of hardwood crumbses, 15 parts in wheat bran, 6 parts of corn flours, 6 parts of bean powderes, 1 part in gypsum, 0.5 part in magnesium sulfate, 0.6 part in zinc sulphate, 0.1 part of 6-BA, 1 part in brown sugar, 1 part of lime, 0.5 part of potassium dihydrogen phosphate, fertile 1 part of rich selenium, 65 parts in water, pH value are 7;
Method for making: after the mentioned component mixing and stirring bottling or the pack, seal, under 120～135 ℃ of temperature, pressure 0.1～0.2Mpa condition, sterilized 2 hours, the female kind of the rich selenium Phellinus of aseptic inoculation, 25 ℃ of unglazed cultivation and fermentation of constant temperature 40～50 days, mycelia is covered with, and promptly gets original seed;
3) preparation of three grades of kinds; With step 2) pedigree seed culture medium inoculation original seed promptly make three grades of kinds;
4) preparation of sclerotinite; With step 2) three grades of kinds of pedigree seed culture medium inoculation promptly make the selenium-rich phellinus igniarius rod, i.e. sclerotinite or fruit body.
Described rich selenium fertilizer is meant one or more in sodium selenite, sodium selenate or the calcium selenate.
Extract the method for Phellinus selenium polysaccharide with prepared selenium-rich phellinus igniarius plastid: selenium-rich phellinus igniarius plastid or fruit body powder are broken into the particle that particle diameter is 0.2-0.6mm; use water logging then; be after fully stirring under 70-80 ℃ the condition in temperature, filter, concentrate, drying promptly makes the Phellinus selenium polysaccharide.
Embodiment 2:
The cultivation method of rich selenium Phellinus, this method comprises the steps:
1) the female preparation of planting of rich selenium Phellinus; The female kind of rich selenium Phellinus is to be made by weight ratio by following compositions: 150 parts of potatos, 30 parts in agar, 30 parts of glucose, 5 parts of bean powderes, 1 part in magnesium sulfate, 0.5 part in zinc sulphate, 10 parts of potassium dihydrogen phosphates, 10 parts of peptones, 0.5 part of tryptophan, 6-BA0.5 part, fertile 1 part of rich selenium, mulberry leaves or 50 parts of Ramulus Mori, 500 parts in water;
Method for making: potato is cleaned section, and mulberry leaves or Ramulus Mori are cleaned, and mix with other composition, and after mixing, slow fire boils to be endured; With 8 layers of filtered through gauze, filtrate packing test tube is made mother culture media after the agar dissolving, medium was sterilized 20～40 minutes under 110～130 ℃ of temperature, pressure 0.1～0.15Mpa condition, aseptic inoculation Phellinus bacterial strain, 25 ℃ of unglazed cultivations of constant temperature 15 days can obtain described female the kind;
2) preparation of rich selenium Phellinus original seed; Rich selenium Phellinus original seed is to be made by weight ratio by following compositions: 30 parts of mulberry tree wood chips, 30 parts of hardwood crumbses, 10 parts in wheat bran, 10 parts of corn flours, 10 parts of bean powderes, 1.5 parts in gypsum, 1 part in magnesium sulfate, 1 part in zinc sulphate, 0.15 part of 6-BA, 1.5 parts in brown sugar, 1.5 parts of limes, 1 part of potassium dihydrogen phosphate, fertile 1.5 parts of rich selenium, 70 parts in water, pH value are 7;
Method for making: after the mentioned component mixing and stirring bottling or the pack, seal, under 120～135 ℃ of temperature, pressure 0.1～0.2Mpa condition, sterilized 2 hours, the female kind of the rich selenium Phellinus of aseptic inoculation, 25 ℃ of unglazed cultivation and fermentation of constant temperature 40～50 days, mycelia is covered with, and promptly gets original seed;
3) preparation of three grades of kinds; With step 2) pedigree seed culture medium inoculation original seed promptly make three grades of kinds;
4) preparation of sclerotinite; With step 2) three grades of kinds of pedigree seed culture medium inoculation promptly make the selenium-rich phellinus igniarius rod, i.e. sclerotinite or fruit body.
With the method for prepared selenium-rich phellinus igniarius plastid extraction Phellinus selenium polysaccharide, with embodiment 1.
Embodiment 3:
The female kind of rich selenium Phellinus is to be made by weight ratio by following compositions: 250 parts of potatos, 10 parts in agar, 10 parts of glucose, 15 parts of bean powderes, 3 parts in magnesium sulfate, 1.5 parts in zinc sulphate, 1 part of potassium dihydrogen phosphate, 1 part of peptone, 1.5 parts of tryptophans, 6-BA1.5 part, fertile 5 parts of rich selenium, mulberry leaves or 150 parts of Ramulus Mori, 1500 parts in water;
Rich selenium Phellinus original seed is to be made by weight ratio by following compositions: 40 parts of mulberry tree wood chips, 40 parts of hardwood crumbses, 20 parts in wheat bran, 2 parts of corn flours, 1 part of bean powder, 0.5 part in gypsum, 0.1 part in magnesium sulfate, 0.1 part in zinc sulphate, 0.05 part of 6-BA, 0.5 part in brown sugar, 0.5 part of lime, 0.1 part of potassium dihydrogen phosphate, fertile 0.5 part of rich selenium, 60 parts in water, pH value are 7;
The cultivation method of rich selenium Phellinus, concrete steps are with embodiment 1.
With the method for prepared selenium-rich phellinus igniarius plastid extraction Phellinus selenium polysaccharide, with embodiment 1.
Claims (3)
1, the cultivation method of rich selenium Phellinus is characterized in that this method comprises the steps:
1) the female preparation of planting of rich selenium Phellinus; The female kind of rich selenium Phellinus is to be made by weight ratio by following compositions: 150～250 parts of potatos, 10～30 parts in agar, glucose 10-30 part, 5～15 parts of bean powderes, 1～3 part in magnesium sulfate, 0.5～1.5 part in zinc sulphate, 1～10 part of potassium dihydrogen phosphate, 1～10 part of peptone, 0.5～1.5 part of tryptophan, 6-BA0.5～1.5 part, 1～5 part of rich selenium fertilizer, mulberry leaves or 50～150 parts of Ramulus Mori, 500～1500 parts in water;
Method for making: potato is cleaned section, and mulberry leaves or Ramulus Mori are cleaned, and mix with other composition, and after mixing, slow fire boils to be endured; With 8 layers of filtered through gauze, filtrate packing test tube is made mother culture media after the agar dissolving, medium was sterilized 20～40 minutes under 110～130 ℃ of temperature, pressure 0.1～0.15Mpa condition, aseptic inoculation Phellinus bacterial strain, 25 ℃ of unglazed cultivations of constant temperature 15 days can obtain described female the kind;
2) preparation of rich selenium Phellinus original seed; Rich selenium Phellinus original seed is to be made by weight ratio by following compositions: 30～40 parts of mulberry tree wood chips, 30～40 parts of hardwood crumbses, 10～20 parts in wheat bran, 2～10 parts of corn flours, 1～10 part of bean powder, 0.5～1.5 part in gypsum, 0.1～1 part in magnesium sulfate, 0.1～1 part in zinc sulphate, 0.05～0.15 part of 6-BA, 0.5～1.5 part in brown sugar, 0.5～1.5 part of lime, 0.1～1 part of potassium dihydrogen phosphate, 0.5～1.5 part of rich selenium fertilizer, 60～70 parts in water, pH value are 7;
Method for making: after the mentioned component mixing and stirring bottling or the pack, seal, under 120～135 ℃ of temperature, pressure 0.1～0.2Mpa condition, sterilized 2 hours, the female kind of the rich selenium Phellinus of aseptic inoculation, 25 ℃ of unglazed cultivation and fermentation of constant temperature 40～50 days, mycelia is covered with, and promptly gets original seed;
3) preparation of three grades of kinds; With step 2) pedigree seed culture medium inoculation original seed promptly make three grades of kinds;
4) preparation of sclerotinite; With step 2) three grades of kinds of pedigree seed culture medium inoculation promptly make the selenium-rich phellinus igniarius rod, i.e. sclerotinite or fruit body.
2, the cultivation method of rich selenium Phellinus according to claim 1 is characterized in that: described rich selenium fertilizer is meant one or more in sodium selenite, sodium selenate or the calcium selenate.
3, extract the method for Phellinus selenium polysaccharide with claim 1 or 2 prepared selenium-rich phellinus igniarius plastids; it is characterized in that: selenium-rich phellinus igniarius plastid or fruit body powder are broken into the particle that particle diameter is 0.2-0.6mm; use water logging then; be after fully stirring under 70-80 ℃ the condition in temperature, filter, concentrate, drying promptly makes rich selenium Phellinus polysaccharide.
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| CN101983955A (en) * | 2010-08-13 | 2011-03-09 | 浙江省农业科学院 | Phellinus igniarius mycelium culture medium and process for fermenting phellinus igniarius using same |
| CN103598010A (en) * | 2013-11-12 | 2014-02-26 | 淳安县微生物研究所 | Original ecological imitative wild cultivation method for inonotus sanghuang |
| CN103739348A (en) * | 2013-12-09 | 2014-04-23 | 柳城新天地生态农业发展有限公司 | Pleurotus geesteranus high-yield strain |
| CN103756912A (en) * | 2013-12-20 | 2014-04-30 | 江苏科技大学 | Phellinus igniarius rejuvenation culture medium |
| CN104381009A (en) * | 2014-09-18 | 2015-03-04 | 杭州千岛湖桑之宝农业开发有限公司 | Artificial bag cultivation process for Phellinus igniarius |
| CN106069176A (en) * | 2016-06-06 | 2016-11-09 | 湖南极硒生物科技有限公司 | Inoculation method rich in organic selenium Lentinus Edodes |
| CN106105770A (en) * | 2016-06-20 | 2016-11-16 | 湖南极硒生物科技有限公司 | Inoculation method rich in organic selenium Pleurotus eryngii |
| CN106472104A (en) * | 2015-08-31 | 2017-03-08 | 付红敏 | A kind of manufacture method of Phellinus igniarius (L. ex Fr.) Quel. cultivation |
| CN109260350A (en) * | 2018-10-31 | 2019-01-25 | 福建农林大学 | A kind of Phellinus mixture and preparation method thereof |
| CN109429892A (en) * | 2018-09-10 | 2019-03-08 | 山东禹泽医药科技有限公司 | A kind of Phellinus selenium-rich bacterium powder |
| CN109601245A (en) * | 2018-12-21 | 2019-04-12 | 兰顺明 | A kind of culture medium and its implantation methods of mulberry tree soak weed tree sawdust factory culture Phellinus |
| CN110358689A (en) * | 2019-08-26 | 2019-10-22 | 福建生物工程职业技术学院 | A kind of selenium-rich coarse wool fibre pore fungi culture medium for mycelial growth |
| CN111484566A (en) * | 2020-04-26 | 2020-08-04 | 潍坊医学院 | Selenium-rich phellinus igniarius polysaccharide and preparation method and application thereof |
| CN112514738A (en) * | 2020-10-31 | 2021-03-19 | 贵州省农作物品种资源研究所(贵州省现代中药材研究所) | Production method of high-quality phellinus igniarius strain |
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| CN1720785B (en) * | 2004-08-12 | 2010-09-15 | 陈全勇 | An artificial high-yield cultivation method of male agaric and application of fruit body thereof |
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- 2006-02-24 CN CNB2006100459037A patent/CN100399877C/en not_active Expired - Fee Related
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101983955A (en) * | 2010-08-13 | 2011-03-09 | 浙江省农业科学院 | Phellinus igniarius mycelium culture medium and process for fermenting phellinus igniarius using same |
| CN103598010A (en) * | 2013-11-12 | 2014-02-26 | 淳安县微生物研究所 | Original ecological imitative wild cultivation method for inonotus sanghuang |
| CN103598010B (en) * | 2013-11-12 | 2015-04-29 | 淳安县微生物研究所 | Original ecological imitative wild cultivation method for inonotus sanghuang |
| CN103739348A (en) * | 2013-12-09 | 2014-04-23 | 柳城新天地生态农业发展有限公司 | Pleurotus geesteranus high-yield strain |
| CN103756912A (en) * | 2013-12-20 | 2014-04-30 | 江苏科技大学 | Phellinus igniarius rejuvenation culture medium |
| CN104381009A (en) * | 2014-09-18 | 2015-03-04 | 杭州千岛湖桑之宝农业开发有限公司 | Artificial bag cultivation process for Phellinus igniarius |
| CN104381009B (en) * | 2014-09-18 | 2016-04-20 | 杭州千岛湖桑之宝农业开发有限公司 | Technique planted by the artificial bag of a kind of Phellinus |
| CN106472104B (en) * | 2015-08-31 | 2020-04-03 | 付红敏 | Manufacturing method for phellinus igniarius cultivation |
| CN106472104A (en) * | 2015-08-31 | 2017-03-08 | 付红敏 | A kind of manufacture method of Phellinus igniarius (L. ex Fr.) Quel. cultivation |
| CN106069176A (en) * | 2016-06-06 | 2016-11-09 | 湖南极硒生物科技有限公司 | Inoculation method rich in organic selenium Lentinus Edodes |
| CN106105770A (en) * | 2016-06-20 | 2016-11-16 | 湖南极硒生物科技有限公司 | Inoculation method rich in organic selenium Pleurotus eryngii |
| CN109429892A (en) * | 2018-09-10 | 2019-03-08 | 山东禹泽医药科技有限公司 | A kind of Phellinus selenium-rich bacterium powder |
| CN109260350A (en) * | 2018-10-31 | 2019-01-25 | 福建农林大学 | A kind of Phellinus mixture and preparation method thereof |
| CN109601245A (en) * | 2018-12-21 | 2019-04-12 | 兰顺明 | A kind of culture medium and its implantation methods of mulberry tree soak weed tree sawdust factory culture Phellinus |
| CN110358689A (en) * | 2019-08-26 | 2019-10-22 | 福建生物工程职业技术学院 | A kind of selenium-rich coarse wool fibre pore fungi culture medium for mycelial growth |
| CN110358689B (en) * | 2019-08-26 | 2021-04-20 | 福建生物工程职业技术学院 | Selenium-rich coarse chaetomium fortune hypha growth medium |
| CN111484566A (en) * | 2020-04-26 | 2020-08-04 | 潍坊医学院 | Selenium-rich phellinus igniarius polysaccharide and preparation method and application thereof |
| CN112514738A (en) * | 2020-10-31 | 2021-03-19 | 贵州省农作物品种资源研究所(贵州省现代中药材研究所) | Production method of high-quality phellinus igniarius strain |
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Granted publication date: 20080709 Termination date: 20180224 |