CN106105770A - Inoculation method rich in organic selenium Pleurotus eryngii - Google Patents

Inoculation method rich in organic selenium Pleurotus eryngii Download PDF

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CN106105770A
CN106105770A CN201610444556.9A CN201610444556A CN106105770A CN 106105770 A CN106105770 A CN 106105770A CN 201610444556 A CN201610444556 A CN 201610444556A CN 106105770 A CN106105770 A CN 106105770A
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pleurotus eryngii
organic selenium
class inoculum
inoculation method
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罗忠理
程立和
钟义凡
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Hunan Polar Selenium Biotechnology Co Ltd
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Hunan Polar Selenium Biotechnology Co Ltd
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    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G18/00—Cultivation of mushrooms
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05D—INORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C; FERTILISERS PRODUCING CARBON DIOXIDE
    • C05D3/00—Calcareous fertilisers
    • C—CHEMISTRY; METALLURGY
    • C05—FERTILISERS; MANUFACTURE THEREOF
    • C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
    • C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor

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  • General Engineering & Computer Science (AREA)
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Abstract

The present invention provides a kind of inoculation method rich in organic selenium Pleurotus eryngii.The described inoculation method rich in organic selenium Pleurotus eryngii includes: step one, screening first class inoculum；Step 2, screening second class inoculum；Step 3, screening three-class strain；And step 4, the described three-class strain filtered out is seeded to pleurotus eryngii cultivating material cultivate.The inoculation method rich in organic selenium Pleurotus eryngii that the present invention provides, has stopped the most artificial foliage spray inorganic selenium, the situation of external forced raising selenium constituent content；Make the utilization rate rich in organic selenium pleurotus eryngii quel strains selenium element that filters out and conversion ratio selenium high, resistance to and stability strong；Cultivate product quality and yield is double high.

Description

Inoculation method rich in organic selenium Pleurotus eryngii
Technical field
The present invention relates to domestic fungus cultivating technical field, be specifically related to a kind of inoculation method rich in organic selenium Pleurotus eryngii.
Background technology
Selenium is one of trace element of needed by human, enjoys the U.S.s such as " long-lived element ", " anticancer king ", " natural antidote " Reputation, its action principle mainly has two: the first, form internal antioxidase-glutathion peroxidase, and protection cell membrane is exempted from Oxidative damage, keeps its permeability；The second, selenium-P albumen has the poisonous substances such as chelating heavy metal, reduces the effect of poisonous substance toxicity. Research proves, compared with inorganic selenium, organic selenium has the advantages such as toxicity is little, easily absorbed by animal, environmental pollution is little, has relatively High practical value.Wherein, for carrier, inorganic selenium being converted into organic selenium with edible fungi is that the mankind are in mushroom field of health care food One of study hotspot.
In the cultivation of selenium-enriched edible mushroom, presently, there are problems with: the quality of (1) strain is the best.Strain excellent The quality of the bad quality being directly connected to organic selenium product and the height of yield, mycelial growth rate is fast, strong stress resistance, long-pending selenium The strain that ability is strong, sporophore quality is good, yield is high, is the top priority of organic selenium edible fungus production.(2) selenium-rich eats The culture medium used in the cultivation of bacterium contains certain density selenium, has inhibitory action for many dominant bacteria growths.
The known edible fungi of China has kind more than 350, wherein belongs to Basidiomycotina more, common are Pleurotus eryngii, Volvariella volvacea (Bull.Ex Franch.) Singer．, silver Ear, mushroom, Tricholoma matsutake (lto et lmai) Singer etc..Pleurotus eryngii is nutritious, the mineral such as rich in proteins, carbohydrate, vitamin and calcium, magnesium, copper, zinc Matter, can improve immune function of human body, has the effects such as anticancer, blood fat reducing, intestine moistening stomach and beauty treatment to human body.Due to Pleurotus eryngii Above-mentioned functions effect, all effects of organic selenium in addition, for carrier, inorganic selenium being converted into organic selenium with Pleurotus eryngii becomes The mankind are at the study hotspot of mushroom field of health care food.
There is the resistance to selenium of advantage pleurotus eryngii quel strains in the inoculation method rich in organic selenium Pleurotus eryngii and stability is not strong, training at present The problem that foster based component not science, culture medium cost height, cultivation complexity, cultivation product quality and yield are the highest.
Therefore, it is necessary to the inoculation method of existing organic selenium Pleurotus eryngii is developed further, to avoid above-mentioned lacking Fall into.
Summary of the invention
There is the resistance to selenium of advantage pleurotus eryngii quel strains to solve the above-mentioned inoculation method rich in organic selenium Pleurotus eryngii and stablize Property strong, medium component not science, culture medium cost high, cultivation complicated, cultivates product quality and the highest the asking of yield Topic, the present invention provides a kind of inoculation method rich in organic selenium Pleurotus eryngii, and it has the suitable Growth of Pleurotus eryngii of medium component, excellent The double high advantage of the Pleurotus eryngii mass rate of production that gesture pleurotus eryngii quel strains stability is strong, inoculation method simple and nurtures.
The present invention provides a kind of inoculation method rich in organic selenium Pleurotus eryngii, comprises the following steps:
Step one, screening first class inoculum:
To cultivate in the spore inoculating of Pleurotus eryngii to first class inoculum culture medium, filtering out the fastest spore of the speed of growth is one Level strain, wherein said first class inoculum culture medium includes following component: Rhizoma Solani tuber osi, agar, glucose, potassium dihydrogen phosphate, magnesium sulfate, Vitamin B1, sodium selenite and water；
Step 2, screening second class inoculum:
Being seeded to described first class inoculum in second class inoculum compost cultivate, filtering out the fastest spore of the speed of growth is two Level strain, wherein said second class inoculum compost includes following component: wheat berry, calcium carbonate, Gypsum Fibrosum, sodium selenite and water；
Step 3, screening three-class strain:
It is seeded to described second class inoculum in described second class inoculum compost continue to cultivate, filters out the speed of growth the fastest Spore is three-class strain, and described three-class strain is rich in organic selenium pleurotus eryngii quel strains；
Step 4, by described rich in organic selenium pleurotus eryngii quel strains be seeded in pleurotus eryngii cultivating material cultivate, wherein said Fructus Pruni Abalone mushroom compost includes following component: weed tree sawdust, wheat bran, calcium carbonate, sucrose, sodium selenite and water.
In a kind of preferred embodiment of the inoculation method rich in organic selenium Pleurotus eryngii of present invention offer, described one-level bacterium Planting culture medium is culture medium based on potato culture, and adds following component in potato culture: biphosphate Potassium 7.6～8.8g/L, agar 20～30g/L, magnesium sulfate 4.21～4.78g/L, vitamin B10.046～0.064g/L, sub-selenium Acid sodium 0.021～0.045g/L.
In a kind of preferred embodiment of the inoculation method rich in organic selenium Pleurotus eryngii of present invention offer, described one-level bacterium Component and the concentration of kind of culture medium is: potassium dihydrogen phosphate 8g/L, magnesium sulfate 4.5g/L, vitamin B10.06g/L, sodium selenite 0.035g/L。
In a kind of preferred embodiment of the inoculation method rich in organic selenium Pleurotus eryngii of present invention offer, described two grades of bacterium Kind of compost includes the component of following mass fraction: wheat berry 120～140 parts, Gypsum Fibrosum 1.95～2.64 parts, calcium carbonate 0.8～ 1.5 parts and sodium selenite 0.0076～0.0096 part.
In a kind of preferred embodiment of the inoculation method rich in organic selenium Pleurotus eryngii of present invention offer, described two grades of bacterium Kind of compost includes the component of following mass fraction: wheat berry 130 parts, 2.14 parts of Gypsum Fibrosum, calcium carbonate 1 part and sodium selenite 0.0086 part.
In a kind of preferred embodiment of the inoculation method rich in organic selenium Pleurotus eryngii of present invention offer, described two grades of bacterium The wheat berry that kind compost contains is ripe wheat berry.
In a kind of preferred embodiment of the inoculation method rich in organic selenium Pleurotus eryngii of present invention offer, described Pleurotus eryngii Compost includes the component of following mass percent: weed tree sawdust 25.83～30.75%, wheat bran 8.48～9.95%, Gypsum Fibrosum 0.31 ～0.43%, sucrose 0.27～0.39%, sodium selenite 0.0095%～0.0109% and water 60～65%.
In a kind of preferred embodiment of the inoculation method rich in organic selenium Pleurotus eryngii of present invention offer, described Pleurotus eryngii Compost includes the component of following mass percent: weed tree sawdust 27.74%, wheat bran 9.5%, Gypsum Fibrosum 0.38%, sucrose 0.38%, Sodium selenite 0.0106% and water 62%.
Compared to existing method, the inoculation method rich in organic selenium Pleurotus eryngii that the present invention provides has following useful effect Really:
One, by the spore of Pleurotus eryngii through multistage screening so that filter out is first rich in selenium in organic selenium pleurotus eryngii quel strains Element utilization rate and conversion ratio selenium high, resistance to and stability strong；
Two, the proportioning of described first class inoculum culture medium, described second class inoculum compost and described pleurotus eryngii cultivating material is suitableeer Preferably rich in the growth of organic selenium pleurotus eryngii quel strains, culture medium cost is low, cultivation is simple, cultivate product quality and yield is double high；
Three, the inoculation method rich in organic selenium Pleurotus eryngii provided by the present invention, substitutes traditional artificial foliage spray Inorganic selenium, external forced improve the mode of selenium constituent content, can avoid that inorganic selenium toxicity is big, safety is low, poor stability, It is difficult to shortcomings such as being absorbed by the body, selenium supplement efficiency is low.
Detailed description of the invention
Technical scheme in the embodiment of the present invention will be clearly and completely described below, it is clear that described enforcement Example is only a part of embodiment of the present invention rather than whole embodiments.Based on the embodiment in the present invention, this area is common All other embodiments that technical staff is obtained under not making creative work premise, broadly fall into present invention protection Scope.
Pleurotus eryngii belongs to low form strain, and northern area can be arranged in spring, autumn two season cultivation.
Pleurotus eryngii typically uses two kinds of types of rearing: flatrack erects pocket type and the horizontal pocket type of vertical frame.
Flatrack erects pocket type:
This mode is for the purpose of utilizing freezer original frame bed, to reduce cost of investment.The bedstead of freezer is generally 6 layers, Bedstead layer away from 60 centimetres, bedside 120 centimetres.Practical cultivated area is calculated as: the mushroom house area x60%x bedstead number of plies, as mushroom house is put down Face area 100m2X60%x6 layer frame=360m2, use wide 20 centimetres, the single head of long 32 centimetres goes out mushroom bag, every packed wet feed 1.2 kilograms, every 1m2 bedstead can discharge 80 bags, and this mushroom house about can cultivate 28000 bags, and average every 1m2 mushroom house can discharge 280 bags.Cold Storehouse mushroom house must carry out three transformations:
(1) to establish ventilation window near the ground, the bidirectional fan of installing band shutter newly, in order to get rid of heavier CO in time2Gas Body.
(2) suitable illumination is the essential condition of the normal fruiting of Pleurotus eryngii, thus every layer of mushroom bed daylight lamp to be set up, intensity with Dark place reaches 300-500Lx for degree.
(3) high-pressure water pipe to be set up in mushroom house, in order to install sprayer unit, improves air humidity.Again, it is preferred to there is draining Floor drain, in order to draining-off sewage, cleaning ambient.
The horizontal pocket type of vertical frame:
It is applicable to booth, the advantage using vertical frame horizontal stroke bag:
(1) it is easy to arrange light, with angle steel material, without frame plate, investment-saving.
(2) it is easy to pass through, swing bag, bag, adopts the operation management intuitive and convenient such as mushroom.
(3) average every 1m2Mushroom house can pile up 178 bags.Data are as follows:
The long 15m of mushroom house, wide 9m, area of plane 135m2.The high long 12m=30m of 2.5m x is set2Slotted-angle shelving 8 row, swing bag is total Plane about 240m2。
Frame width 0.2m, every 0.7m height sets horizontal steel fine strain of millet, and every 1m is wide sets perpendicular steel 1, frame spacing 0.9m.
Double end is used to go out out mushroom bag, wide 17 centimetres, long 34 centimetres, every packed wet feed 1.2 kilograms, every 1m2Bedstead is arranged Putting 100 bags, this mushroom house about can cultivate 24000 bags, average every 1m2Mushroom house piles up 178 bags.
The described inoculation method rich in organic selenium Pleurotus eryngii, comprises the following steps:
Step S1, screening first class inoculum:
To cultivate in the spore inoculating of Pleurotus eryngii to first class inoculum culture medium, filtering out the fastest spore of the speed of growth is First class inoculum, wherein said first class inoculum culture medium includes following component: Rhizoma Solani tuber osi, agar, glucose, potassium dihydrogen phosphate, sulphuric acid Magnesium, vitamin B1, sodium selenite and water.
Described first class inoculum culture medium is culture medium based on potato culture, and adds in potato culture Following component: potassium dihydrogen phosphate 7.6～8.8g/L, magnesium sulfate 4.21～4.78g/L, vitamin B10.046～0.064g/L, Asia Sodium selenate 0.021～0.045g/L.
And specifically, it is preferable to the component of the described first class inoculum culture medium of scheme and concentration are: potassium dihydrogen phosphate 8g/L, sulphuric acid Magnesium 4.5g/L, vitamin B10.06g/L, sodium selenite 0.035g/L；It is prepared in accordance with the following steps:
Step S11, configures potato culture: weigh peeled potatoes 215g, chip, put into aluminum pot, adds 1000mL water, boils 12min again after boiling；Obtain filtrate by 4-6 layer filtered through gauze, filtrate is refunded aluminum pot, add agar 26g, stir Mix addition glucose 26g, potassium dihydrogen phosphate 8g, magnesium sulfate 4.5g, vitamin B after dissolving to agar10.06g and sodium selenite 0.035g；Pour graduated cylinder while hot into, add water and complement to 1000mL, prepare fluid medium.
Take the test tube that prepared fluid medium loads multiple 10mL, install culture medium, test tube mouth tampon to be jumped a queue, add cotton After plug, test tube is bundled into one, with kraft paper, the one end being plugged with tampon is wrapped, tighten with cotton rope, test tube is stood up at wire basket In, in case sterilizing.
Step S12, autoclaving: add 3000-35000ml water in portable pressure cooker, little wire basket is placed in aluminum In cylinder, at little which floor newspaper of wire basket upper cover, build lid, tighten heating.Water boiling final vacuum 5-10 minute, turns off vent valve Door, makes pressure be gradually increasing.When pressure reaches 1.1kg/cm2Time, start timing, after maintaining constant voltage half an hour, close thermal source, allow Pressure slowly drops to zero.
This step also can use other suitable sterilization methods.
Step S13, puts inclined-plane: after opening lid, treat that temperature is down to 65 DEG C, rest on batten by one section of test tube mouth, another End is put on the table, becomes as constant slope.By culture medium cool down after inclined-plane, chamfer length is generally the 2/ of test tube length 5--1/2, then moves in calorstat, prepares described first class inoculum culture medium.
Step S14, strain extracts: use in spore separation method, base any one side among partition method or tissue isolation Method extracts spore from Pleurotus eryngii.
Step S15, connects bacterium: after extracting Pleurotus eryngii spore, migrate in described first class inoculum culture medium so that it is sprout, certainly By copulation；Cultivate under conditions of 35 DEG C, treat Sporulation mycelia.
Step S16, screens, tames, purifies: treat that Sporulation mycelia, the spore that wherein mycelial growth rate is the fastest are Described first class inoculum, is seeded to (concentration of sodium selenite rises to 0.5g/L) in another pipe first class inoculum culture medium and is continued Cultivate, after mycelia covers with test tube, select best as first class inoculum, move to save backup at 1 DEG C-4 DEG C in calorstat.
Step S2, screening second class inoculum:
It is seeded to the described first class inoculum filtered out in step S1 in second class inoculum compost cultivate, filters out growth speed Spending the fastest spore is second class inoculum, and wherein said second class inoculum compost includes following component: wheat berry, calcium carbonate, Gypsum Fibrosum, Sodium selenite and water.
Described second class inoculum compost contains the raw material components of following mass fraction: wheat berry 120～140 parts, Gypsum Fibrosum 1.95～2.64 parts, calcium carbonate 0.8～1.5 parts and sodium selenite 0.0076～0.0096 part.
Select the raw material components that the described second class inoculum compost of excellent scheme contains following mass fraction: wheat berry 130 parts, stone Cream 2.14 parts, calcium carbonate 1 part and sodium selenite 0.0086 part.
Concrete, select the mildew and rot rotten ripe wheat berry 910g of full seed, nothing, clean, soak 10-20 hour； With after decocting in water to boiling 6-8 minute.Filtering off excessive moisture, airing 2-3 hour is not to dripping；Add Gypsum Fibrosum 15g and carbonic acid Calcium 7g mixes thoroughly；Sodium selenite 0.06g is put into and water sprays after dilution and mixes thoroughly；Take seed bottle subpackage, be filled to every strain bottle Long-pending about 1/2-2/3, prepares described second class inoculum culture medium.
Step S21, autoclaving: after filling, should autoclaving immediately.Horizontal high-pressure autoclave, dress height can be used During pressure autoclave, bottleneck upward, puts not tension.After the boiling of pressure cooker water, first open vent valve 8 minutes, then shut.Pressure Control at 1.4kg/cm2Left and right, keeps constant temperature two hours.Then, thermal source is closed, when being cooled to zero, pot can be opened Stove.This step also can use other sterilization methods.
Step S22, inoculation: under sterile conditions, described first class inoculum is seeded to second class inoculum compost, in temperature Being to cultivate at 25 DEG C, after treating that mycelia is covered with, selecting spore excellent, pollution-free, that mycelial growth rate is the fastest is described two grades Strain.
Step S3, screening three-class strain:
The described second class inoculum filtered out in step S2 is seeded to described second class inoculum compost continue to cultivate, filters out The fastest spore of the speed of growth is three-class strain, and described three-class strain is rich in organic selenium pleurotus eryngii quel strains.
Step S4, will step S3 filter out described in be seeded in pleurotus eryngii cultivating material rich in organic selenium pleurotus eryngii quel strains Cultivating, wherein said pleurotus eryngii cultivating material includes following component: weed tree sawdust, wheat bran, Gypsum Fibrosum, sucrose, sodium selenite and water.
Described pleurotus eryngii cultivating material contains the raw material components of following mass percent: weed tree sawdust 26.83～30.75%, bran Skin 7.38～8.45%, Gypsum Fibrosum 0.504～0.576%, sucrose 0.17～0.19%, sodium selenite 0.00095%～ 0.00109% and water 60～65%.
Preferred described pleurotus eryngii cultivating material includes the component of following mass percent: weed tree sawdust 27.74%, wheat bran 9.5%, Gypsum Fibrosum 0.38%, sucrose 0.38%, sodium selenite 0.0106% and water 62%.Specifically include following steps:
Step S41, by weed tree sawdust 730kg, wheat bran 250kg, sucrose 10kg, calcium carbonate 10kg and sodium selenite 280g, spray Spill its surface, push away through repeatedly turning over, make the water content of compost reach 62%, prepare pleurotus eryngii cultivating material.
After the water content of the described pleurotus eryngii cultivating material prepared in step S42, regulating step S41 is about 68%, charging. Go out mushroom bag, every packed wet feed 1.2 kilograms according to single head, prepare bacterium rod.The described bacterium rod preferably collar adds tampon, in order to thoroughly It is fast that gas sends out bacterium well.Go out mushroom bag, every packed wet feed 1.2 kilograms according to double end, prepare bacterium rod, described bacterium rod bag folding mouth or jag ?.
Early autumn cultivation can be selected for Polypropylene Bag, and this bag of transparency is good, and pocket is through high temperature sterilize aftercrimp, without empty between coating materials Gap, is not likely to produce interlayer mushroom, but the easy fragmentation of winter low temperature, preferably select high density low-pressure polyethylene bag.
Step S43, by described bacterium rod atmospheric steam at steam sterilization 2 hours, or 100 DEG C under the pressure of 1.5kg/cm2 Sterilizing 16 hours.Inoculation is described rich in the described bacterium rod after organic selenium pleurotus eryngii quel strains to sterilization, the temperature of 15 DEG C-20 DEG C Under, ventilation and lucifuge, carry out sending out bacterium.
Going out mushroom bag according to single head, 1 bottle of strain can inoculate 30 bags, is uprightly thrown on the bedstead sending out bacterium room after inoculation.
Mushroom bag, 1 bag of strain inoculation 15-20 bag is gone out according to double end.Because bacterium bag tying is without tampon, treat that mycelial growth reaches material More than 1/2 time, appropriate loosening bag mouth, increase oxygen promote mycelial growth.
Step S44, treat that the described mycelia rich in organic selenium pleurotus eryngii quel strains that step S43 is seeded on described bacterium rod is complete After covering with, convert through 5-8d and cultivate.
Step S45, it is 65% in air humidity, under conditions of temperature is 25 DEG C, the two ends of bacterium rod opened, waits described Growing sporophore rich in organic selenium pleurotus eryngii quel strains, described sporophore is i.e. rich in organic selenium Pleurotus eryngii.
Open bag: open bag when mycelia is not yet twisted together, it is difficult to forming former base or former base is formed very slow, fruiting is irregular, mushroom body Economic characters are poor；Open bag when former base is formed or small mushroom bud occurs, the differentiation of former base and little mushroom grow normal, and fruiting is neat, mushroom body Economic characters good；If open bag when sporophore is grown up, there will be misshapen mushroom in bag, the mushroom grown time serious can wither Contract, rot.What therefore Pleurotus eryngii planted by bag opens a bag time, should rest in mycelia kink and form former base occurred opening during small mushroom bud Bag, solves opened mouth, by folding under outside for bag film turnup to being advisable higher than charge level 2cm.
Temperature control manages: mushroom house temperature directly affects the formation of former base and the growth promoter of sporophore.
Temperature less than 8 DEG C time, former base is difficult to be formed, be i.e. the mushroom body grown also can stop growing, atrophy, flavescence until Dead；When temperature continues more than 18 DEG C, oneself mushrooms out suddenly by the sporophore of differentiation, and quality can decline；Temperature more than 20 DEG C Time, mycelia turns to again and nourishes and grows, and the effect of low temperature stimulation also just disappears, former base stasi, the nutrition in mushroom flower bud flow backwards and Wilting, established children mushroom also can atrophy death.Therefore, fruiting phase mushroom house temperature should control at 13-15 DEG C, and such fruiting is fast, Mushroom flower bud is many, and fruiting is neat, within about 15 days, can gather.
Moisturizing manages: mushroom house air humidity should be maintained at 85%-95%, and humidity is the lowest, and sporophore meeting atrophy, former backbone splits Can not break up, in order to improve air humidity, the most handy mister head spray water upward, it is sure not to be sprayed onto by water on mushroom body, otherwise Sporophore can turn yellow affects quality, can cause rotten time serious.
Ventilating management: the former base period then needs the oxygen of abundance, CO2Concentration should be reduced to about 0.5%, the most former base regardless of Change and enlarge into spherical.The mushroom bulk-growth period of development, it is also desirable to air is fresh, CO2Concentration is advisable with not higher than 0.4%.
If fruiting phase improper ventilation, due to CO2 excessive concentration, it may appear that misshapen mushroom, if running into high temperature, high humidity sky again Gas, also results in sporophore and rots.Therefore, good ventilation condition must be kept in fruiting phase mushroom house, particularly work as mushroom When flower bud occurs in a large number, stretch bacterium bag mouth thin film in time, to avoid mushroom flower bud lopsided because of anoxia.
Illumination management: the growth and development stage of existing former base and sporophore needs certain scattered light, and suitable intensity of illumination is 300-500Lx.Every layer or often row bedstead all should have suitable illumination, it is impossible to have dead angle.
The inoculation method rich in organic selenium Pleurotus eryngii that the present invention provides has a following beneficial effect:
One, by the spore of Pleurotus eryngii through multistage screening so that filter out is first rich in selenium in organic selenium pleurotus eryngii quel strains Element utilization rate and conversion ratio selenium high, resistance to and stability strong；
Two, the proportioning of described first class inoculum culture medium, described second class inoculum compost and described pleurotus eryngii cultivating material is suitableeer Preferably rich in the growth of organic selenium pleurotus eryngii quel strains, culture medium cost is low, cultivation is simple, cultivate product quality and yield is double high；
Three, the inoculation method rich in organic selenium Pleurotus eryngii provided by the present invention, substitutes traditional artificial foliage spray Inorganic selenium, external forced improve the mode of selenium constituent content, can avoid that inorganic selenium toxicity is big, safety is low, poor stability, It is difficult to shortcomings such as being absorbed by the body, selenium supplement efficiency is low.
The foregoing is only embodiments of the invention, not thereby limit the scope of the claims of the present invention, every utilize this Equivalent structure or equivalence flow process that bright description is made convert, or are directly or indirectly used in other relevant technology neck Territory, is the most in like manner included in the scope of patent protection of the present invention.

Claims (8)

1. the inoculation method rich in organic selenium Pleurotus eryngii, it is characterised in that comprise the following steps:
Step one, screening first class inoculum:
To cultivate in the spore inoculating of Pleurotus eryngii to first class inoculum culture medium, filtering out the fastest spore of the speed of growth is one-level bacterium Kind, wherein said first class inoculum culture medium includes following component: Rhizoma Solani tuber osi, agar, glucose, potassium dihydrogen phosphate, magnesium sulfate, dimension are raw Element B1, sodium selenite and water；
Step 2, screening second class inoculum:
Being seeded to described first class inoculum in second class inoculum compost cultivate, filtering out the fastest spore of the speed of growth is two grades of bacterium Kind, wherein said second class inoculum compost includes following component: wheat berry, calcium carbonate, Gypsum Fibrosum, sodium selenite and water；
Step 3, screening three-class strain:
It is seeded to described second class inoculum in described second class inoculum compost continue to cultivate, filters out the spore that the speed of growth is the fastest For three-class strain, described three-class strain is rich in organic selenium pleurotus eryngii quel strains；
Step 4, by described rich in organic selenium pleurotus eryngii quel strains be seeded in pleurotus eryngii cultivating material cultivate, wherein said Pleurotus eryngii Compost includes following component: weed tree sawdust, wheat bran, calcium carbonate, sucrose, sodium selenite and water.
Inoculation method rich in organic selenium Pleurotus eryngii the most according to claim 1, it is characterised in that described first class inoculum is trained Foster base is culture medium based on potato culture, and adds following component in potato culture: potassium dihydrogen phosphate 7.6 ～8.8g/L, agar 20～30g/L, magnesium sulfate 4.21～4.78g/L, vitamin B10.046～0.064g/L, sodium selenite 0.021～0.045g/L.
Inoculation method rich in organic selenium Pleurotus eryngii the most according to claim 2, it is characterised in that described first class inoculum is trained Support the component of base and concentration is: potassium dihydrogen phosphate 8g/L, magnesium sulfate 4.5g/L, vitamin B10.06g/L, sodium selenite 0.035g/L。
Inoculation method rich in organic selenium Pleurotus eryngii the most according to claim 1, it is characterised in that described second class inoculum is trained Nutriment includes the component of following mass fraction: wheat berry 120～140 parts, Gypsum Fibrosum 1.95～2.64 parts, calcium carbonate 0.8～1.5 parts With sodium selenite 0.0076～0.0096 part.
Inoculation method rich in organic selenium Pleurotus eryngii the most according to claim 4, it is characterised in that described second class inoculum is trained Nutriment includes the component of following mass fraction: wheat berry 130 parts, 2.14 parts of Gypsum Fibrosum, calcium carbonate 1 part and sodium selenite 0.0086 Part.
Inoculation method rich in organic selenium Pleurotus eryngii the most according to claim 4, it is characterised in that described second class inoculum is trained The wheat berry contained in nutriment is ripe wheat berry.
Inoculation method rich in organic selenium Pleurotus eryngii the most according to claim 1, it is characterised in that described Pleurotus eryngii is cultivated Material includes the component of following mass percent: weed tree sawdust 25.83～30.75%, wheat bran 8.48～9.95%, Gypsum Fibrosum 0.31～ 0.43%, sucrose 0.27～0.39%, sodium selenite 0.0095%～0.0109% and water 60～65%.
Inoculation method rich in organic selenium Pleurotus eryngii the most according to claim 7, it is characterised in that described Pleurotus eryngii is cultivated Material includes the component of following mass percent: weed tree sawdust 27.74%, wheat bran 9.5%, Gypsum Fibrosum 0.38%, sucrose 0.38%, sub-selenium Acid sodium 0.0106% and water 62%.
CN201610444556.9A 2016-06-20 2016-06-20 Inoculation method rich in organic selenium Pleurotus eryngii Pending CN106105770A (en)

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