CN1810295A - Agricultural waste deodorizing bacterial prepn and its production process - Google Patents
Agricultural waste deodorizing bacterial prepn and its production process Download PDFInfo
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Abstract
The agricultural waste deodorizing bacterial preparation is prepared through the following steps: the slant test tube culture, liquid seed culture and tertiary extension culture of giant bacillus, micro xanthoderma and Koningii Trichoderma to prepare three kinds of pure bacterial preparation; inoculating the three kinds of pure bacterial preparation in the equal ratio and inoculated amount of 0.2 % to carrier culture medium and culturing at water content of 55-60 % and temperature 28-34 deg.c for 7 days; and final drying the compound bacterial preparation at 35-45 deg.c and live bacteria inspection. The bacterial preparation of the present invention has obvious deodorizing effect and fast acting.
Description
Technical field
The present invention relates to microbial technology field, specifically be applicable to the preparation method of a kind of a kind of agricultural wastes deodorizing microorganism of eliminating agricultural wastes foul smell such as feces of livestock and poultry, agricultural crop straw and products thereof.
Background technology
Along with scale, the intensification of the fast development of modern agriculture, particularly animal husbandry are produced, cause extensive the concentrating of agricultural wastes such as a large amount of feces of livestock and poultry to be thrown aside; Because development of urbanization trend is quickened, and the generation of domestic waste is surprising, and the organic principle ratio is very high in agricultural wastes and the house refuse, form complicated simultaneously.Contain a large amount of pathogenic microorganism and parasitic ovum in these organic wastes, in the process of throwing aside, can produce a large amount of foul gass, mainly comprise ammonia, hydrogen sulfide, indole, mercaptan, thioether, formaldehyde, acetaldehyde, acrylic aldehyde, methylamine, ethamine, phenol, thiophenol and volatile fatty acid etc., environment for human survival is polluted, serious harm urban and rural residents healthy.In order effectively to address this problem, there are many deodorizing microorganism products to come out both at home and abroad, mainly in the majority with the chemical deodorizing microbial inoculum, mainly be pH regulator agent, oxidant or antibacterial; Part microbial deodorant microbial inoculum is also arranged, as enzymatic microorganism and EM microbial inoculum, the alleviation in various degree of these products the generation of stink of organic waste, but effect is not ideal, is not accepted by market.
Summary of the invention
At above-mentioned the deficiencies in the prior art part, it is low to the purpose of this invention is to provide a kind of cost, easy to use, nonpollution environment, has no side effect, and deodorizing microorganism has significant effect to the foul gas of eliminating agricultural wastes and producing, and can reduce H
2S and NH
3A kind of agricultural wastes deodorizing microorganism of burst size.
Another object of the present invention provides a kind of preparation method of agricultural wastes deodorizing microorganism product.
A kind of agricultural wastes deodorizing microorganism of the present invention is because following microorganism: bacillus megaterium (Bacillus megaterium) CGMCC:1.223, thin Rhizoma Coptidis mycete (Streptomycesmicroflavus) CGMCC:4.891, healthy and free from worry Trichoderma spp. (Trichoderma koningii) CGMCC:3.2774 are 0.5～1.5: 0.5～1.5 by ratio of quality and the number of copies: 0.5～1.5.
Above-mentioned three kinds of microorganism public can buy at China Committee for Culture Collection of Microorganisms common micro-organisms center.
The technical scheme that a kind of agricultural wastes deodorizing microorganism is adopted follows these steps to realize:
1. technological process of production figure (see figure 1);
2. culture medium:
2.1 potato agar culture medium: peeling Rhizoma Solani tuber osi 200g, glucose 20g, agar 15～20g, 15 pounds of 30min sterilizations;
2.2 Gao Shi starch agar culture medium: potassium nitrate 1g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium chloride 0.5g, ferrous sulfate 10mg, soluble starch 20g, agar 20g, cold water 1000mg, PH7.2～7.4,8 pound 30min sterilization;
2.3 nutrition nutrient agar culture medium: peptone 10g, beef extract 3.0g, sodium chloride 10g, agar 15g, distilled water 1000ml, PH7.0,15 pounds of 30min sterilizations;
2.4 solid support medium: zeolite 50%, wheat bran 20%, bone meal 14%, fish flour 14%, brown sugar 2%;
3. three kinds of microbial strains that the present invention is used are inoculated in respectively and carry out the one-level cultivation on potato agar culture medium, Gao Shi starch agar culture medium and three kinds of culture medium of nutrition nutrient agar culture medium; Be inoculated in respectively then in the fluid medium (not adding agar) of above-mentioned three kinds of culture medium, carry out liquid secondary amplification culture, just prepare three kinds of liquid bacterial agents of forming microbial strains.
With above-mentioned three kinds of pure microbial inoculums of microorganism with 1: 1: 1 ratio co-inoculation in solid support medium, inoculum concentration is 0.2%, control culture medium moisture was cultivated 7 days under 28-34 ℃ of condition at 55-60%;
5. carry out 35-45 ℃ of dried with above-mentioned again through the composite bacteria agent capable of cultivating, doing just becomes product of the present invention after the viable bacteria check meets the agricultural industry criteria NY 335-1998 of the People's Republic of China (PRC).
The present invention compared with prior art has the following advantages:
1. microorganism composite deodurizing microbial inoculum of the present invention can effectively increase agricultural wastes micro organism quantity during the fermentation, improves the temperature of fermentation body rapidly, reduces the foul smell of agricultural wastes, thereby promotes the decomposition speed of agricultural wastes.
2. add microorganism composite deodurizing microbial inoculum deodorizing effect of the present invention.
This microorganism composite deodurizing microbial inoculum nonpollution environment, have no side effect.
4. use microorganism composite deodurizing microbial inoculum of the present invention, keep during the fermentation handling low NH than other
4 +-N concentration and other are handled high NO
3 -N concentration helps reducing stink and increases the nitrogen retention rate.
Description of drawings
Fig. 1 complex micro organism fungicide preparation technology figure;
The variations in temperature of Fig. 2 solid fermentation material (℃) figure;
Fig. 3 different disposal compost fermentation deodorising effect comparison diagram;
Fig. 4 different disposal is at the ammonia release concentration figure of different time;
Fig. 5 different disposal is at the H of different time
2S release concentration figure;
The temperature variation of Fig. 6 different disposal composting fermentation process;
The pH value variation diagram of each processing of Fig. 7 in composting fermentation process;
The NH of Fig. 8 different disposal windrow
4 +-N content is with the variation diagram of compost time;
The NO of Fig. 9 different disposal windrow
3 --N content is with the variation diagram of compost time;
The coliform group count variation diagram in Figure 10 different disposal fermentation reactor system each period.
The specific embodiment
In conjunction with Figure of description, below in conjunction with embodiment content of the present invention is described further, but the present invention is not limited to following examples.
1 one kinds of agricultural wastes deodorizing microorganisms of embodiment
Be made up of bacillus megaterium, thin Rhizoma Coptidis mycete, healthy and free from worry Trichoderma spp., the ratio of quality and the number of copies of above-mentioned said three kinds of microorganisms is 0.5～1.5: 0.5～1.5: 0.5～1.5.
The method of the smelly microbial inoculum that embodiment 2 preparation embodiment 1 described agricultural wastes remove
1. preparation culture medium
1.1 potato agar culture medium: peeling Rhizoma Solani tuber osi 200g, glucose 20g, agar 15～20g, 15 pounds of 30min sterilizations.
1.2 Gao Shi starch agar culture medium: potassium nitrate 1g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium chloride 0.5g, ferrous sulfate 10mg, soluble starch 20g, agar 20g, cold water 1000mg, PH7.2～7.4,8 pound 30min sterilization.
1.3 nutrition nutrient agar culture medium: peptone 10g, beef extract 3.0g, sodium chloride 10g, agar 15g, distilled water 1000ml, PH7.0,15 pounds of 30min sterilizations.
1.4 solid support medium: zeolite 50%, wheat bran 20%, bone meal 14%, fish flour 14%, brown sugar 2%.
3. buy thin Rhizoma Coptidis mycete, thermoactinomyces vulgaris, three kinds of each 1 pipes of koning trichoderma from China Committee for Culture Collection of Microorganisms common micro-organisms center, above-mentioned three kinds of microbial strains are cultivated and three grades of amplification culture by test tube slant cultivation, liquid seeds respectively, prepared the pure microbial inoculum of three kinds of bacterial strains.
With above-mentioned three kinds of pure microbial inoculums of microorganism with 0.5: 1: 1.5 ratio co-inoculation in solid support medium, inoculum concentration is 0.2%, control culture medium moisture was cultivated 7 days under 32 ℃ of conditions 58%.
5. carry out 35-45 ℃ of dried with above-mentioned again through the composite bacteria agent capable of cultivating, doing just becomes product of the present invention after the viable bacteria check meets the agricultural industry criteria NY 335-1998 of the People's Republic of China (PRC).
3 one kinds of agricultural wastes deodorizing microorganisms of embodiment
Formed in 1: 1: 1 by weight percentage by bacillus megaterium, thin Rhizoma Coptidis mycete, healthy and free from worry Trichoderma spp..
Experimental example 4 prepares the method for the smelly microbial inoculum of embodiment 3 described agricultural wastes with embodiment 1.
5 one kinds of agricultural wastes deodorizing microorganisms of embodiment
Formed in 1.5: 1: 0.5 by weight percentage by bacillus megaterium, thin Rhizoma Coptidis mycete, healthy and free from worry Trichoderma spp..
The experiment of experimental example 1 deodorizing effect
1 test material and method
1.1 culture medium
Beef-protein medium, Gause I synthetic medium, Cha Shi synthetic medium, yeast extract protein culture medium.
1.2 pedotheque
Soil below the soil below the chicken manure heap, wheat straw heap
The main component of table 1 composting material
| Material | Moisture (%) | Organic carbon (%) | Full nitrogen (%) | Carbon-nitrogen ratio |
| The chicken manure wheat straw | 71 13.5 | 30.146 39.9 | 2.338 0.65 | 14.0 66.5 |
1.3 experimental design
The decomposition material
This experiment is composting substrate with the wheat straw, makes the conditioner of compost fermentation with chicken manure, and it is long that wheat straw is cut up with a hay cutter into 3cm.
The specification of solid-state fermentation tank
The high 150cm of fermentation tank, internal diameter 80cm, external diameter 90cm, air inlet is arranged at the bottom, and top is outlet, and inside and outside interlayer is a heat-barrier material, can on-line monitoring variations in temperature and consumption rate.
Experiment is handled
With a certain amount of wheat straw and chicken manure mix homogeneously, adjust both ratios, making the carbon-nitrogen ratio C/N of mixture is 31: 1, and adding water, to make mixture moisture content be 65%, and three processing are established in experiment, that is:
Handle 1:, do not add any microbial bacterial agent for contrast (CK);
Handle 2: add the complex micro organism fungicide CM1 that other factory prepares, the CM1 addition is 1: 100 (W/V).
Handle 3: add the complex micro organism fungicide CM2 that implementation column 1 prepares, the CM2 addition is 1: 100 (W/V).
1.4 measuring
Measuring deodorizing effect adopts the sensory feel method that the abnormal smells from the patient of solid fermentation material is measured (judge).
Measure variations in temperature and discuss METHOD FOR CONTINUOUS DETERMINATION variations in temperature and record with ZDR-11 solid state temperature record.
Oxygen consumption rate BY-84H type O
2Analyzer is measured oxygen content.With maximum oxygen supply speed [1L/ (minkg)] oxygen supply 20min, use mouthful oxygen content more than 20%, close the oxygen supply valve then, measure oxygen rate of change in time, both be keto consumpting speed.
Measure micropopulation dynamic change (CFU)
Adopt microorganism pure culture method to measure the CFU Changing Pattern of various microorganisms in the solid fermentation material.
Chemical index (C/N)
Water-soluble carbon adopts potassium bichromate titrimetric method-outer heating; Full nitrogen adopts H
2SO
4-salicylic acid one salt-mixture the cooking method that disappears; Ammonium nitrogen (NH
4 +-N) adopt the alkaline hydrolysis diffusion method.
Germination index (GI)
Getting fermentation material fresh sample by liquid manure than lixiviate in 5: 1, absorption 5ml lixiviating solution places the culture dish that is lined with filter paper, and contrast (distilled water) is set simultaneously, put 10 full wheat seeds in each culture dish, 20 ℃ of constant temperature culture 24 hours, bear germination percentage and root is long, calculate GI.GI is determined by following formula:
GI (%)=[(the percentage of seedgermination * seminal root of compost lixiviating solution is long)/(root of the percentage of seedgermination * seed of distilled water is long)] * 100
1.5 result and discussion
Abnormal smells from the patient
The abnormal smells from the patient of table 2 solid fermentation material changes
| The decomposition time (my god) | ||||||||||
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | |
| CK CM1 CM2 | +++ +++ +++ | +++ ++ ++ | ++ + + | ++ + + | ++ - - | + - - | + - - | + - - | + - - | + - - |
Annotate: "-" is not smelly; "+" is little smelly; " ++ " is more smelly; " +++" very smelly
As can be found from Table 2, add complex microbial inoculum after, just make the fermentation material not produce stink through 5 days fermentations, and that tester still has is little smelly through 10 days fermentation, illustrate that the deodorizing effect of complex microbial inoculum is obvious.
Described before combining, complex microbial inoculum CM1 and CM2 can effectively increase wheat straw and the micro organism quantity of chicken manure in the solid fermentation process, improve the temperature of fermentation body rapidly, reduce the foul smell of chicken manure, thereby promote the decomposition speed of wheat straw and chicken manure.
The experiment of experimental example 2 deodorizing effects
1. materials and methods
Strains separation culture medium beef-protein medium, Gause I, Czapek's medium, bean sprout juice sucrose medium, good gas cellulose-decomposing bacterium culture medium; Separation energy is utilized the microbiological culture media of mercaptan compound fast.
The test raw material chicken manure is taken from the fresh excrement on the same day of Xibei Univ. of Agricultural ﹠ Forest Science ﹠ Technology's experimental plot chicken house; Wheat stalk is that farm, this school is when the air-dry wheat straw of producing per year; The zymocyte song is thin Rhizoma Coptidis mycete; Deodorizing microorganism is for using product of the present invention.
Table 6 test raw material basis
| Raw material | Moisture content (%) | Nitrogen (gkg -1) | Carbon (gkg -1) | Carbon-nitrogen ratio/(C/N) |
| The chicken manure wheat straw | 72.3 10.5 | 32.6 6.27 | 218.4 453.3 | 6.7 72.3 |
1.2 method
With deodorizing microorganism embodiment 1 prepare as this test.
EXPERIMENTAL DESIGN
(1) fermentation tank specification fermentation tank is the vertical type cylinder type, diameter 100cm, high 150cm.
(2) the i.e. three groups of fermentation tanks while compost of 3 processing are established in the test processing, and handle I (CK) and be: chicken manure+wheat straw, processing II is: chicken manure+wheat straw+zymocyte song, processing III is: chicken manure+wheat straw+deodorizing microorganism.All handle the C/N that all regulates compound with wheat straw, and making C/N is 31: 1.Adding water, to make mixture moisture content be 65%.
(3) the gravity-flow ventilation static fermentation is boxed out on fermentation reactor system method fermentation tank bottom and top.
Sampling and mensuration
(1) pack into and respectively sample 1 time with the position fermentation tank same day and the 3rd, 5,7,10,15,20,25,30,35 day in sampling time and position.The sampling position is the following 50cm of charge level.
(2) the stink index determining adopts six grades of classification method to divide the smelly degree of gas, record when each sampling.
Ms0: no stink; Ms1: feel stink reluctantly; Ms2: faint stink; Ms3: tangible stink; Ms4: very strong stink; Ms5: insufferable stink.
(3) ammonia and sulfuration carrier of oxygen are measured with GS-3 type atmospheric sampling machine and are gathered the air sample according to the sampling time, absorb the amount that Kjeldahl method is measured ammonia with boric acid, measure the amount of hydrogen sulfide with zinc ammonium complex salt Absorptiometry.
(4) temperature is set every 1 hour the temperature at the following 50cm of record charge level place automatically with TDR-21 type automatic temperature recorder.
(5) the mensuration total number of bacteria of microbiology index adopts flat board to pour into mixing method; Total number of fungi adopts colony counting method; The actinomycetes sum adopts colony counting method; Coliform group count adopts the lactose fermentation method.
(6) the organic calcination loss of weight method that adopts of the mensuration of chemical index; PH tries with the pH value instrumentation; The magnesium oxide way of distillation is measured NH
4 +-N; Ultraviolet method is measured NO
3 --N; Full phosphorus of atomic absorption spectroscopy determination and full potassium.
1.3 interpretation of result
Deodorising effect
Six grades of classification of artificial olfactory sensation are measured each deodorising effect of handling and are seen Fig. 3:
As seen from Figure 3, handle II and handle III and all reached the Ms5 level at the 3rd day, handling the 5th day stink of III begins to weaken, handle II and also be in the most smelly stage, handling III did not promptly have stink at the 15th day, handle II and do not have stink in the time of 20 days, and processing I (CK) the stink persistent period is the longest, in the time of the 7th day, also be in the most smelly stage, just be destitute of smell during by 35 days; The processing III that adds deodorizing microorganism can reach the compost odorlessization quickly than handling II and handling I (CK), and processing III is than handling II, handling I (CK) 5 days ahead of time and 20 days elimination stinks respectively; The time of staying of handling III smelly degree more than the MS4 level is than handling II, handling I (CK) short 3 days and 13 days respectively.Illustrate behind the adding deodorizing microorganism better deodorising effect is arranged.
Each handles the release influence to ammonia
Ammonia, hydrogen sulfide, mercaptan and methanthiol etc. are the main components of feces composting phase foul smell.Find out by Fig. 4, each earlier fermentation that is released in of handling ammonia all has the release of short-term to increase the phase, time length is different variant because of handling, and the increase phase that adds the processing III of deodorizing microorganism is 0～3d, and what add fermenting agent is to handle II to increase the phase at 0-5 days with the release of handling I (CK).Whole fermentation process ammonia release concentration is to handle I (CK)＞processing II＞processing III in proper order, researchs such as Chen Shuan are thought will reduce the ammonia burst size, time should be chosen in the 0-15d, and more early deodorizing effect is good more, the processing III ammonia in 0-10d that adds deodorizing microorganism discharges mean concentration and has descended 21% and 46% respectively than handling II and handling I (CK), can reduce the generation of stink.
Each handles the releasing effect to hydrogen sulfide
H
2S will reach desirable deodorizing effect as reducing one of main target gas of stacking foul smell, needs to control within 10d H
2The release of S is learnt by Fig. 5, handles III and handles II with the H that handles I (CK)
2The release period basically identical of S all is lower than processing I (CK) but handle III with the release concentration of handling II, handles the H of III at 0-10d
2The burst size of S reduces the speed maximum, and therefore, the processing III that adds deodorizing microorganism has shown H
2The release of S has the effect of short-term, low concentration.
Deodorizing microorganism is to the influence of composting process
The variations in temperature of composting process is handled III and is promptly risen to more than 60 ℃ the 3rd day temperature referring to Fig. 6, handles II and also reaches 60 ℃ the 4th day to the 5th day temperature, handles III and has showed the characteristics of handling II, handling I (CK) quick heating.The maximum temperature of handling II is higher 1.5 ℃ than the maximum temperature of handling III, but handle III the persistent period more than 60 ℃ than handling II the longer duration more than 60 ℃ 4 days.Keep 60 ℃ of high temperature more than 3 days, more help effectively killing pathogenic bacterium, help destroying macromolecular substances simultaneously, make windrow reach better rotten degree.Handling III has 10 days megathermal periods more than 60 ℃, will transform and windrow harmless treatment generation positive role the windrow material.
The microorganism species variation sees Table 7.
All kinds of micro organism quantities of table 7 different times change
| Sampling time | Total number of bacteria | The actinomycetes sum | Total number of fungi | ||||||
| Handle I | Handle II | Handle III | Handle I | Handle II | Handle III | Handle I | Handle | Handle III | |
| 0 1 3 5 7 10 15 20 25 30 35 | 10.21 11.09 10.43 9.21 8.86 8.04 7.43 6.62 6.77 7.03 7.21 | 10.83 11.40 10.76 10.32 9.68 9.42 9.07 9.83 9.86 9.23 9.34 | 10.62 11.73 10.94 10.66 9.23 9.11 9.05 9.24 9.26 9.56 9.03 | 7.64 7.68 8.23 8.32 8.11 8.04 8.15 8.13 7.56 7.32 7.18 | 7.92 8.15 8.34 8.56 8.46 8.12 8.10 8.28 7.94 7.85 7.63 | 7.87 8.24 8.65 8.71 8.52 8.38 8.24 8.18 8.09 8.03 7.81 | 8.32 8.61 8.26 7.84 6.97 7.12 7.08 6.84 6.72 7.16 7.14 | 8.54 9.06 8.41 8.09 7.57 6.21 6.36 6.94 7.11 7.23 7.34 | 8.39 9.17 8.86 8.41 8.06 6.89 6.84 6.65 6.94 7.18 7.31 |
Annotate: numeral is microbial count (individual g in the table
-1Windrow) logarithm value.
Can find out from table 7, dissimilar micro organism quantity differences in three kinds of processing, during the fermentation beginning, what micro organism quantity was maximum during each was handled is antibacterial, secondly is fungus and actinomycetes.At the 1st day of fermentation, all micro organism quantities increased sharply, but can find out and expect warm higher period (3-15d), and micro organism quantity is on a declining curve.Along with the decline of material temperature, micro organism quantity gos up to some extent afterwards, but has not reached the level of microorganism when beginning compost.During the fermentation, no matter be middle thermophase or hot stage, the total v of number of bacteria is maximum.During the fermentation, antibacterial is a dominant microflora, and fermentation calefaction is had bigger effect, actinomycetes are more active at hot stage, utilize the Organic substance of undecomposed difficult degradation still, carry out growth and breeding, quantity increases gradually, it is the main flora of this stage decomposing organic matter, the processing III that adds deodorizing microorganism, in the bacterial number maximum of temperature rise period, variance analysis shows and handles I (CK), handle the significant difference (P＜0.05) between II, reached the fastest intensification effect; At hot stage, each actinomycetes quantity of handling all is higher than the quantity after heat up preceding and the cooling, the actinomycetes quantity of this phase process III plays positive role greater than the quantity of handling I (CK) and processing II to keeping continuous high temperature, and the significant difference (P＜0.05) between each processing.
The pH value of sweat changes
The pH value of test changes as shown in Figure 7, the time dependent basic trend of the pH value of three kinds of processing is identical, along with the carrying out of sweat, microorganism is in the early stage bred in a large number, the decomposing protein type organic, produce ammonia nitrogen, impel pH value to go up, along with the protein Organic substance is further degraded, ammoniacal nitrogen is converted into nitrate nitrogen under the effect of nitrobacteria, pH value progressively falls after rise, and the pH value of three kinds of processing maintains between the 6.4-7.1 when fermentation ends.
According to the research of recklessly wearing, tame feces pH value is between 7～8, and deodorizing effect is good, and pH value is lower than 6 or be higher than 8.5 and all do not reach the deodorant purpose.The pH value scope that adds the processing III of deodorizing microorganism and the processing II that adds the zymocyte song is all in this zone, and the fluctuation of the pH value of processing III more helps deodorization and mass degradation less than processing II and processing I (CK).
The variation of nitrogen class material
Contain many nitrogen class materials in the animal dung, in composting process, they are degraded to NH fast
4 +-N.Part NH
4 +-N is NO by microbial transformation further
3 --N and organic nitrogen, major part have little time the NH that transformed
4 +-N volatilizees with gaseous state in the environment of pH＞7.The main loss approach that this is not only N in the compost, also be the poultry compost mainly cause smelly reason.Nitrogen class material is with NH in the control composting process
4 +The accumulation of-N form is stink control and the key point that improves N nutrient retention rate
[22]
Find out that by Fig. 8, Fig. 9 each handles NH
4 +The content of-N rose to some extent at 0-3 days, continues to reduce thereupon, since the existence of a large amount of nitrifier and denitrifying bacterium, the NH of organic nitrogen mineralising
4 +-N changes into NO by nitrifier
3 --N content improves rapidly.Part NO
3 --N is become N by denitrification again
2Gas, or be fixed to microorganism nitrogen by fungus effect, make NO
3 --N content decreases.In this experiment, after 3d, the NH that each is handled
4 +-N content reduces gradually, has reduced NH under the alkali condition
3Volatilization concentration, each in period NH
4 +-N concentration shows that in proper order for handling III＜processing II＜processing I (CK) handling III can reach better deodorizing effect.At fermentation NO at the end
3 --N content to handle the highest of III, is handled II and is taken second place, and it is minimum to handle I (CK), has illustrated that the processing III that adds deodorizing microorganism has more excellent nitrogen retention rate.
1.2.2.3 deodorizing microorganism is to the influence of compost quality
Nutrient content before and after the fermentation reactor system changes
The organic matter of three kinds of processing, total nitrogen, full phosphorus, full potassium all increase before and after the fermentation, organic, full nitrogen amplification maximum is the processing III that adds deodorizing microorganism, its amplification is respectively 18.6% and 14.6%, secondly for handling II, amplification is respectively 16.2% and 13.2%, handle I (CK) amplification minimum, and the amplification significant difference between three kinds of processing.The full phosphorus of different disposal, full potassium amplification maximum for handling II, secondly for handling III, handle I (CK) amplification minimum, handle II and handle the full phosphorus of III, full potassium with handling I (CK) amplification significant difference, but the difference of handle II, handling between III is not remarkable, therefore, handle the increase of III, shown comprehensive advantage the back nutrient that ferments.
Nutrient before and after the table 8 different disposal fermentation reactor system changes (unit: gkg
-1)
| Organic | Total nitrogen | Full phosphorus/P 2O 5 | Full potassium K 2O | |||||||||
| Before the fermentation | After the fermentation | Increase and decrease amplitude/% | Before the fermentation | After the fermentation | Increase and decrease amplitude/% | Before the fermentation | After the fermentation | Increase and decrease amplitude/% | Before the fermentation | After the fermentation | Increase and decrease amplitude/% | |
| Handle I and handle II processing III | 496.3 484.7 487.2 | 564.3 563.2 577.8 | +13.7a +16.2b +18.6c | 23.4 23.8 23.6 | 26.0 26.9 27.0 | +11.3a +13.2b +14.6c | 18.3 18.9 18.7 | 20.5 22.0 21.6 | +12.1a +16.4b +15.6b | 9.3 9.5 9.1 | 10.1 10.4 9.9 | +8.7a +10.1b +9.4b |
The hygiology index of compost
As shown in Figure 8, along with the prolongation of course of fermentation, coliform progressively descends, by 10 of the compost initial stage
5The order of magnitude of individual/gram fermentation material drops to 10
2Individual/gram, the following order of magnitude of fermentation material.The processing III that adds the processing II of zymocyte song and add deodorizing microorganism is in the time of 15 days, and coliform quantity has reduced to 10
2The order of magnitude of/gram fermentation material meets the decontamination of human excreta standard, handles III owing to the time that continues in the megathermal period is long, and coliform monarch minimum number during fermentation ends is 3 a/gram fermentation material, shows more excellent fertilizer completeness.
1.4 conclusion
(1) the processing III of adding deodorize microbial inoculum has deodorizing effect preferably, eliminates stink, MS in 20 days ahead of time than handling I (CK)
4Level is above smellyly to be spent the time of staying and handles II respectively and handled I (CK) short 3 days and 13 days, handles III ammonia in 0～10d and discharges mean concentration than handling II and processing I (CK) descends 21% and 46% respectively.
(2) handle that II handles that the temperature of III in composting process becomes and the health overall performane of compost after finishing meets the decontamination of human excreta standard, handling III than handling II has bigger temperature advantage (the temperature maintenance was Duoed 4 days more than 60 ℃); Handling III keeps handling low NH than other during the fermentation
4 +-N concentration and other are handled high NO
3 --N concentration helps reducing stink and increases the nitrogen retention rate.Handle organic matter before and after the III fermentation and total nitrogen amplification maximum with handle II and handle the significant difference of I (CK), full phosphorus, full potassium amplification is also handled I (CK) that significant difference is arranged.
Claims (2)
1. an agricultural wastes deodorizing microorganism is characterized in that, is made up of bacillus megaterium, thin Rhizoma Coptidis mycete, healthy and free from worry Trichoderma spp., and the ratio of quality and the number of copies of above-mentioned said three kinds of microorganisms is 0.5～1.5: 0.5～1.5: 0.5～1.5.
2. the preparation method of the described a kind of agricultural wastes deodorizing microorganism product of claim 1 is characterized in that being undertaken by following steps:
1) culture medium:
1.1) the potato agar culture medium: peeling Rhizoma Solani tuber osi 200g, glucose 20g, agar 15～20g, 15 pounds of 30min sterilizations;
1.2) Gao Shi starch agar culture medium: potassium nitrate 1g, dipotassium hydrogen phosphate 0.5g, magnesium sulfate 0.5g, sodium chloride 0.5g, ferrous sulfate 10mg, soluble starch 20g, agar 20g, cold water 1000mg, PH7.2～7.4,8 pound 30min sterilization;
1.3) nutrition nutrient agar culture medium: peptone 10g, beef extract 3.0g, sodium chloride 10g, agar 15g, distilled water 1000ml, PH7.0,15 pounds of 30min sterilizations;
1.4) solid support medium: zeolite 50%, wheat bran 20%, bone meal 14%, fish flour 14%, brown sugar 2%;
2) bacillus megaterium, thin Rhizoma Coptidis mycete, three kinds of microbial strains of healthy and free from worry Trichoderma spp. are cultivated and three grades of amplification culture by test tube slant cultivation, liquid seeds, prepared the pure microbial inoculum of three kinds of bacterial strains;
3) with step 2) in three kinds of pure microbial inoculums of microorganism with 1: 1: 1 ratio co-inoculation in step 1.4) in solid support medium in, inoculum concentration is 0.2%, control culture medium moisture was cultivated 7 days under 28-34 ℃ of condition at 55-60%;
4) with carrying out 35-45 ℃ of dried again through the composite bacteria agent capable of cultivating in the step 3), do promptly to get deodorizing microorganism of the present invention behind the viable bacteria.
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| CN102010824A (en) * | 2010-09-07 | 2011-04-13 | 西北农林科技大学 | Microbial agent for promoting quick decomposition of agricultural wastes and preparation method |
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| CN1370829A (en) * | 2001-02-27 | 2002-09-25 | 广州天蔚生物技术有限公司 | Bacterial spawn for treating domestic organic garbage |
| CN1493683A (en) * | 2003-08-26 | 2004-05-05 | 李鸣雷 | Microorganism composite fungus agent for promoting agricultural waste quickly rotten and its making method |
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