CN1810102A - Fruit fly culturing process and culturing case - Google Patents

Fruit fly culturing process and culturing case Download PDF

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Publication number
CN1810102A
CN1810102A CN 200510022297 CN200510022297A CN1810102A CN 1810102 A CN1810102 A CN 1810102A CN 200510022297 CN200510022297 CN 200510022297 CN 200510022297 A CN200510022297 A CN 200510022297A CN 1810102 A CN1810102 A CN 1810102A
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China
Prior art keywords
case lid
casing
trypetid
fruit
vent
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Granted
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CN 200510022297
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Chinese (zh)
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CN100469241C (en
Inventor
陈志粦
余道坚
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Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry Exit Inspection and Quarantine Bureau
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Animal and Plant Inspection and Quarantine Technology Center of Shenzhen Entry Exit Inspection and Quarantine Bureau
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Priority to CNB2005100222972A priority Critical patent/CN100469241C/en
Publication of CN1810102A publication Critical patent/CN1810102A/en
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Publication of CN100469241C publication Critical patent/CN100469241C/en
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Abstract

The fruit fly culturing process includes the following steps: 1.setting fruit with fruit fly egg or larva inside closed culturing case possessing ventilation hole covered with 1 mm below mesh gauze; and 2. culturing for 7-16 days to make the larva or egg grow into adult larva. The fruit fly culturing case includes a case body and a case cover with ventilation hole covered with 1 mm below mesh gauze. The present invention is favorable to the ventilated preservation of fruit inside the case and can avoid escape of fruit fly larva.

Description

A kind of method and a kind of trypetid incubator of cultivating the fruit trypetid
Technical field
The present invention relates to cultural method and the incubator of a kind of insect, the incubator that particularly relates to the cultural method of a kind of fruit trypetid and be used to cultivate the fruit trypetid.
Background technology
Along with further opening and accession to WTO of China, in recent years fruit international trade is increasing, and China in 2004 carries out zero tariff to Thailand's import fruit, import fruit in Taiwan is also carried out this preferential policy in 2005, therefore, trade import fruit variety and quantity all have significantly increases.Port, Shenzhen (comprise Luohu, Huang Gang, Wen Jin cross and Shatoujiao etc.) entry passenger is again a national best, and the passenger carries the fruit immigration and reaches ten thousand batches of 3-4 every year; These fruit partly are the torrid zone and subtropical fruits, Philippine, Thailand, Malaysia and Vietnam etc. that major part is inferior southeast.This area is the serious generating region of bactrocera oligochaeta, and trypetid kind complexity, especially bactrocera oligochaeta (Bactrocera spp.) are not only of a great variety, and numerous species is again the important pests that fruit is produced.In addition, some fruit has the danger that passes band " the No.1 killer of fruits and vegetables " Mediterranean fruitfly from states such as Mediterranean fruitfly epidemic-stricken area such as the U.S., Chile, Australia, South Africa.In recent years, port, Shenzhen has been intercepted and captured quarantine dangerous insect such as Mediterranean fruitfly, Queensland trypetid and Philippine trypetid etc. of one, two classes and has been reached more than 5000 batch from import fruit quarantine, 22 kinds of trypetids, and most of kind does not have the record of distribution as yet in China.
It is to be as the criterion with adult that the identification of morphology of trypetid is generally acknowledged in the world, is based on larva but immigration fruit passes the trypetid worm attitude of band, also can be with ovum sometimes, fruit need be placed and treat in 7-16 days just to identify after larva or ovum are cultivated into adult.Conventional fruit trypetid is cultivated and normally deposits with glue bucket covered and enclosed, still since 1. glue bucket shortcoming circle to occupy spatial volume big, it is few to deposit fruit, unfavorable putting on the shelf carried out fruit in enormous quantities and checked; Add a cover promptly airtightly if 2. deposit fruit, fruit is easily mouldy to rot, and influence interior trypetid larva of pulp or egg development; If 3. not adding a cover trypetid above larva in 3 ages can bounce; 4. the glue bucket is deposited fruit and is taken out of, moves into time-consuming, effort.Therefore, necessary making adapts to fruit examination requirements in enormous quantities, can ventilation extension fruit retention time, favourable trypetid larva or oviparity long hair are educated, can seal again and prevent that insect from going out, and in the case at interval in case different fruit sample room trypetid mature larva spring and infect, and carry easily mutually and adapt to fruit in enormous quantities and put on the shelf and support worm and the fruit trypetid incubator of practicality.
Summary of the invention
Purpose of the present invention is intended to overcome above-mentioned the deficiencies in the prior art, propose a kind of can be favourable fruit preserve and the method for the cultivation fruit trypetid that trypetid larva or oviparity long hair are educated and favourable fruit is preserved and trypetid larva or oviparity long hair are educated fruit trypetid incubator.
For achieving the above object, the present invention has adopted following technical scheme:
The invention discloses a kind of method of cultivating the fruit trypetid, described method comprises step:
(1) fruit that will have doubtful worm's ovum or a young larva is positioned over incubator, this incubator comprises casing and case lid, offer air-vent on the case lid, and air-vent has carried out seal with the gauze that mesh is not more than 1mm, after case lid is placed on the casing, except that described air-vent, casing and case lid remainder are sealing state;
(2) cultivation treated that young larva or egg development were mature larva in 7-16 days.
The condition of culture of described step (2) is 26 ℃~28 ℃ of temperature, cultivates under humidity 60~80% conditions.
In described step (2) afterwards, further comprise step: cut open fruit and take out mature larva and put into the special-purpose worm cylinder of supporting and pupate, and further raise and be adult.
The invention also discloses a kind of trypetid incubator, comprise casing and case lid, described case lid is provided with air-vent, and air-vent carries out seal with the gauze that mesh is not more than 1mm, and after case lid was placed on the casing, except that described air-vent, casing and case lid remainder were sealing state.
Described gauze is the stainless steel gauze.
The periphery of described case lid reverse side is provided with first groove, and this first groove can closely cooperate with the casing apical margin.
Be provided with dismountable movable separation in the described casing, this activity is separated and is used for casing is divided into several spaces independent of each other, and between the inwall of this activity separation and casing, and case lid covers to be between movable apical margin of separating in back and the case lid and closely cooperates.
Air-vent on the described case lid has a plurality of, and after case lid covers, and is separated on the pairing case lid of each separate space of being isolated by activity in the casing to be equipped with air-vent.
The both sides in described case lid front respectively are provided with one second groove, and described air-vent evenly is located in these two second grooves.
Described bottom half is provided with pulley.
Because adopted above scheme, the beneficial effect that the present invention is possessed is:
Method of the present invention and trypetid incubator of the present invention, by air-vent only is set on case lid, and at the fruit trypetid form of being cultivated and the particularity of habit, after the gauze that air-vent selects for use mesh to be not more than 1mm carries out seal, help being placed on the ventilative preservation of fruit in the incubator on the one hand, on the other hand, prevented that effectively fruit trypetid larva from bouncing and go out from incubator.
The periphery of incubator case lid reverse side of the present invention is provided with first groove, and this first groove and casing apical margin closely cooperate, can guarantee that case lid covers after, except air-vent, all the other are sealing state, prevent that trypetid from bouncing from incubator and go out.
Be provided with the activity that casing can be divided into several spaces independent of each other in the casing and separate, thereby make incubator of the present invention can be used for placing simultaneously several fruit samples at one time, increased availability.
Air-vent on the case lid is arranged on second groove in case lid front, thereby and, can be increased in the firmness of the gauze seal on the air-vent so on the one hand, on the other hand with the bonding herein sealing of gauze air-vent, incubator use and stacked process in, gauze is not easy to come off.
Be set to 26 ℃~28 ℃ of temperature by condition of culture, humidity 60~80% has guaranteed to cultivate and has carried out under optimum condition, helps the cultivation of trypetid larva.
Description of drawings
Fig. 1 is the front schematic view of the case lid of a kind of trypetid incubator of the present invention.
Fig. 2 is the structural representation of the casing of a kind of trypetid incubator of the present invention.
Embodiment
Also the present invention is described in further detail in conjunction with the accompanying drawings below by specific embodiment.
See Fig. 1,2.
A kind of fruit trypetid incubator comprises casing 1 and case lid 2, this incubator plastic production, and casing is transparent, and specification is long 48cm * wide 30cm * high 36cm.
Casing 1 apical margin periphery bow is protruding, and 7 one of handles are respectively adorned near top 2cm place in two sides.
The periphery of case lid 2 reverse side is provided with first groove, and when covering case lid on casing, this first groove can closely cooperate with the casing apical margin.In addition, the both sides of case lid 2 reverse side respectively are provided with second a flat groove 5, in these two second grooves 5, be provided with 5 air-vents 3 (every hole φ 3.5cm respectively equably, at a distance of 1.5cm), every hole periphery is coated all-purpose adhesive, uses stainless steel gauze (long 27cm * wide 7cm) seal of a 1mm mesh on two second grooves 5 respectively, make 10 air-vents 3 all be capped (among Fig. 1, gauze does not draw).
Be provided with dismountable movable separation 4 in the casing 1, box house is separated into 6 independently spaces.And after case lid 2 covers, on 6 pairing case lids of separate space air-vent is arranged all.This activity separation 4 is pieced together slotting the making by 3 2mm slabs and is formed, and wherein two specification is: long 30cm * wide 26cm, cut the recess that 2mm is wide, 15cm is dark at long middle 15cm place; Another block specifications: long 41cm * wide 30cm, it is wide respectively to cut 2mm at long 10cm place, both sides, the 15cm deep notch.Three slabs are pieced together mutually at deep notch and are inserted the formation activity and separate 4, the junction of every slabs, and with the junction of cabinet wall, be closely cooperating of sealing.And, the movable separation between 4 apical margin and the case lid 2 after case lid 2 covers also for closely cooperating.Four jiaos of the bottom of casing 1 also are equipped with the pulley of being convenient to carry 6.
After being placed on case lid 2 on the casing 1, except that air-vent 3, casing 1 and case lid 2 remainders all are sealing state.
Utilize above-mentioned incubator, the fruit that will have doubtful worm's ovum or larva is positioned over respectively in the casing 1 to be separated in 4 separate space of being separated by activity, covers case lid, forms only by air-vent on the case lid 23 and the extraneous state that communicates.26 ℃~28 ℃ of temperature, placed under humidity 60~80% conditions 7-16 days, treat that young larva or ovum cultivate into mature larva, cut open fruit then and take out mature larva and put into the special-purpose worm cylinder of supporting and pupate, and further raise the identification of morphology that can be used for adult behind the adult.

Claims (10)

1, a kind of method of cultivating the fruit trypetid, described method comprises step:
(1) fruit that will have doubtful worm's ovum or a young larva is positioned over incubator, this incubator comprises casing and case lid, offer air-vent on the case lid, and air-vent has carried out seal with the gauze that mesh is not more than 1mm, after case lid is placed on the casing, except that described air-vent, casing and case lid remainder are sealing state;
(2) cultivation treated that young larva or egg development were mature larva in 7-16 days.
2, a kind of method of cultivating the fruit trypetid according to claim 1 is characterized in that: the condition of culture of described step (2) is cultivated under humidity 60~80% conditions for 26 ℃~28 ℃ of temperature.
3, a kind of method of cultivating the fruit trypetid according to claim 1 and 2 is characterized in that: in described step (2) afterwards, further comprise step: cut open fruit and take out mature larva and put into the special-purpose worm cylinder of supporting and pupate, and further raise and be adult.
4, a kind of trypetid incubator comprises casing and case lid, it is characterized in that: described case lid is provided with air-vent, and air-vent carries out seal with the gauze that mesh is not more than 1mm, and after case lid was placed on the casing, except that described air-vent, casing and case lid remainder were sealing state.
5, a kind of trypetid incubator according to claim 4, it is characterized in that: described gauze is the stainless steel gauze.
6, a kind of trypetid incubator according to claim 5, it is characterized in that: the periphery of described case lid reverse side is provided with first groove, and this first groove can closely cooperate with the casing apical margin.
7, a kind of trypetid incubator according to claim 6, it is characterized in that: be provided with dismountable movable separation in the described casing, this activity is separated and is used for casing is divided into several spaces independent of each other, and, between the inwall of this activity separation and casing, and case lid covers to be between movable apical margin of separating in back and the case lid and closely cooperates.
8, a kind of trypetid incubator according to claim 7, it is characterized in that: the air-vent on the described case lid has a plurality of, and after case lid covers, and is separated on the pairing case lid of each separate space of being isolated by activity in the casing to be equipped with air-vent.
9, a kind of trypetid incubator according to claim 8, it is characterized in that: the both sides of described case lid reverse side respectively are provided with one second groove, and described air-vent evenly is located in these two second grooves.
10, according to the arbitrary described a kind of trypetid incubator of claim 4~9, it is characterized in that: described bottom half is provided with pulley.
CNB2005100222972A 2005-12-09 2005-12-09 Fruit fly culturing process and culturing case Expired - Fee Related CN100469241C (en)

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Application Number Priority Date Filing Date Title
CNB2005100222972A CN100469241C (en) 2005-12-09 2005-12-09 Fruit fly culturing process and culturing case

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CN100469241C CN100469241C (en) 2009-03-18

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Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103222448A (en) * 2013-02-05 2013-07-31 东莞市盛唐化工有限公司 Breeding method of bactrocera cucurbitae
CN105393987A (en) * 2015-10-30 2016-03-16 黄雅琴 Simple incubator and method for making drosophila melanogaster ride out winter and summer by using same
CN111332620A (en) * 2020-02-21 2020-06-26 广西壮族自治区农业科学院 Method for transporting fruit fly insect fruit sample for long distance

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1067211C (en) * 1995-12-18 2001-06-20 武汉苍龙生物工程有限公司 Commercial cultivating method and equipment for fly maggot
CN1196402C (en) * 2000-06-16 2005-04-13 江来章 Production technique of fast-production regeneration animal protein 'fly larva protein'
CN1149013C (en) * 2000-09-22 2004-05-12 王欧 Method for large scale cultivation of fly maggot

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN103222448A (en) * 2013-02-05 2013-07-31 东莞市盛唐化工有限公司 Breeding method of bactrocera cucurbitae
CN103222448B (en) * 2013-02-05 2015-01-28 东莞市盛唐化工有限公司 Breeding method of bactrocera cucurbitae
CN105393987A (en) * 2015-10-30 2016-03-16 黄雅琴 Simple incubator and method for making drosophila melanogaster ride out winter and summer by using same
CN111332620A (en) * 2020-02-21 2020-06-26 广西壮族自治区农业科学院 Method for transporting fruit fly insect fruit sample for long distance

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