CN105393987A - Simple incubator and method for making drosophila melanogaster ride out winter and summer by using same - Google Patents
Simple incubator and method for making drosophila melanogaster ride out winter and summer by using same Download PDFInfo
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- CN105393987A CN105393987A CN201510716518.XA CN201510716518A CN105393987A CN 105393987 A CN105393987 A CN 105393987A CN 201510716518 A CN201510716518 A CN 201510716518A CN 105393987 A CN105393987 A CN 105393987A
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- incubator
- blake bottle
- fruit bat
- summer
- winter
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- 238000000034 method Methods 0.000 title claims abstract description 21
- 241000255601 Drosophila melanogaster Species 0.000 title abstract description 8
- 238000009395 breeding Methods 0.000 claims abstract description 16
- 230000001488 breeding effect Effects 0.000 claims abstract description 16
- 238000004321 preservation Methods 0.000 claims abstract description 6
- 235000013399 edible fruits Nutrition 0.000 claims description 49
- 241000255581 Drosophila <fruit fly, genus> Species 0.000 claims description 15
- 238000009413 insulation Methods 0.000 claims description 5
- 229920000742 Cotton Polymers 0.000 claims description 4
- 230000000295 complement effect Effects 0.000 claims description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 239000001963 growth medium Substances 0.000 abstract 2
- 239000002609 medium Substances 0.000 description 16
- 238000012360 testing method Methods 0.000 description 7
- 239000000203 mixture Substances 0.000 description 6
- 238000011160 research Methods 0.000 description 6
- XBDQKXXYIPTUBI-UHFFFAOYSA-N dimethylselenoniopropionate Natural products CCC(O)=O XBDQKXXYIPTUBI-UHFFFAOYSA-N 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229920001817 Agar Polymers 0.000 description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 2
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 239000008272 agar Substances 0.000 description 2
- 238000004458 analytical method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 238000013461 design Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 235000019260 propionic acid Nutrition 0.000 description 2
- IUVKMZGDUIUOCP-BTNSXGMBSA-N quinbolone Chemical compound O([C@H]1CC[C@H]2[C@H]3[C@@H]([C@]4(C=CC(=O)C=C4CC3)C)CC[C@@]21C)C1=CCCC1 IUVKMZGDUIUOCP-BTNSXGMBSA-N 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 231100000033 toxigenic Toxicity 0.000 description 2
- 230000001551 toxigenic effect Effects 0.000 description 2
- 238000012549 training Methods 0.000 description 2
- 230000035899 viability Effects 0.000 description 2
- 241000288673 Chiroptera Species 0.000 description 1
- 241000255925 Diptera Species 0.000 description 1
- 241000255582 Drosophilidae Species 0.000 description 1
- 241000892865 Heros Species 0.000 description 1
- 241000500891 Insecta Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 230000000052 comparative effect Effects 0.000 description 1
- 239000000356 contaminant Substances 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 238000012136 culture method Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013312 flour Nutrition 0.000 description 1
- 239000012737 fresh medium Substances 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New or modified breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Biodiversity & Conservation Biology (AREA)
- Cultivation Receptacles Or Flower-Pots, Or Pots For Seedlings (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a simple incubator, comprising a bubble cabinet with an upper cover. The upper part of the bubble cabinet is provided with a support grid plate. The moveable upper cover is provided with holes. A culture bottle passes through the hole of the upper cover and location holes of the support grid plate and extends to the lower part of the bubble cabinet. A bottle opening of the culture bottle is higher than the upper cover. A tampon is arranged in the bottle opening. The method comprises: in winter, placing the incubator on a place facing the sun in a room, detecting breeding condition of the drosophila melanogaster every other week, and changing fresh culture medium as needed; in summer, placing the incubator in a refrigerator preservation chamber at the temperature of 4 DEG C, taking the incubator out and placing in indoor environment for a day every other week, then detecting breeding condition of the drosophila melanogaster, and putting the incubator in a refrigerator to continue culture, at the time of 3-4 week, changing fresh culture medium as needed and subculturing. The bubble cabinet which is low in cost is used as the drosophila melanogaster incubator. The incubator is simple in structure and low in manufacturing cost. The incubator can ensure safe breeding of drosophila melanogaster population when being used in winter vacation or summer vacation, and the incubator satisfies requirements of drosophila melanogaster breed conservation breeding.
Description
Technical field
The present invention relates to genetics and Developmental Biology technology, especially relate to a kind of simple incubator, the invention still further relates to and use this simple incubator that fruit bat degree of safety is passed the winter the method in summer.
Background technology
Drosophila melanogaster (Drosophilamelanogaster), Insecta, diptera, Drosophilidae, be one of biology the most thoroughly of current human research, itself has the features such as breeding is fast, the history of life is short, filial generation number is many, and gene sequencing has completed, be important genetics research model organism, in Genetics Experimental Teaching and scientific research, there is consequence.Be need to carry out conservation to fruit bat in the scientific research of object and education activities to breed with fruit bat, especially some rare mutant line more will ensure that it can expand procreation.Fruit bat to external world temperature is very responsive, when temperature is lower than 5 DEG C, fruit bat can not moved at all, and the time is grown will be dead because of hungry; When temperature is higher than 30 DEG C, fruit bat there will be sterile even dead.Generally, fruit bat is placed in medium to expand cultivation in 25 DEG C of intelligent climatic cabinates by researcher, changes a subculture every about 7.
Because intelligent climatic cabinate is expensive, long-time work double tides need consume a large amount of electric energy, and toxigenic capacity is high, and will produce noise during incubator work, pollutes the environment in compressor cooling process.And under normal circumstances, summer and winter are all peak times of power consumption, cooling and heating will consume the most electric power of China, and at this moment intelligent climatic cabinate also often cannot be able to start because of brownout, affects normal training.
The researcher of institution of higher education has winter vacation and two vacations of summer vacation every year, and interim when winter vacation and summer vacation, teaching and scientific research work will come to an end.If only in order to conservation allows intelligent climatic cabinate work 24 hours every days with the breeding of fruit bat, toxigenic capacity is higher; Also need to configure a collection of fresh medium every about 7, also add workload to researcher.Therefore, need to design a kind of fruit bat degree of safety that can make and to pass the winter the Simple culture method in summer and culture apparatus, the culture environment of a constant temperature can be provided to fruit bat, ensure the procreation of fruit bat population, also want low-carbon economy, simple to operate.
Summary of the invention
The object of the invention is to the defect that exists for prior art and provide a kind of simple incubator, the present invention also provides and uses this simple incubator that fruit bat degree of safety is passed the winter the method in summer.
For achieving the above object, the present invention can take following technical proposals:
Simple incubator of the present invention, comprise casing, described casing is the bubble chamber with movable top lid, the upper level of described bubble chamber is provided with the support screen with locating hole, described movable top lid offers the through hole corresponding with described position of positioning hole, the blake bottle being loaded with Drosophila medium successively from the through hole of movable top lid and support screen locating hole pass and extend to the bottom of described bubble chamber, the bottleneck of described blake bottle, higher than the upper surface of described movable top lid, is provided with breathable insulation tampon in described bottleneck.
Use simple incubator of the present invention that the pass the winter method in summer of fruit bat degree of safety is comprised the steps:
First prepare fresh Drosophila medium, and be divided in blake bottle, make the height of medium be about 1/3 of blake bottle height; The quantity of placing fruit bat in each blake bottle is determined according to the kind of fruit bat, label outside blake bottle, indicate number and the blake bottle numbering of drosophila strains, female male drosophila, then blake bottle is placed in above-mentioned simple incubator, and fill heat-preservation cotton by its complementary space in bubble chamber, close upper cover;
During use in winter, this simple incubator is placed on area without shade in room, week about, takes out the breeding situation that blake bottle checks fruit bat, change fresh culture as required simultaneously;
Summer is when using, this simple incubator is placed on 4 DEG C of fridge freshness retaining indoor, week about, this simple incubator is taken out from refrigerator and to be placed on Indoor Natural environment one day, then the breeding situation that blake bottle checks fruit bat is taken out, put into the indoor continuation of 4 DEG C of fridge freshness retainings again to cultivate, change fresh culture when 3 ~ 4 weeks as required and carry out turning culture.
The invention has the advantages that the heat preservation and insulation utilizing cystosepiment, use cheap bubble chamber as fruit bat incubator, structure is simple, cheap for manufacturing cost, and bubble chamber and the filling heat-preservation cotton in it expands to fruit bat and numerously provides effective thermal environment, be beneficial to and preserve and expand numerous fruit bat species; When using in winter vacation and summer holidays, the safety breeding of fruit bat population can be ensured, meet the demand that fruit bat conservation is bred.
Accompanying drawing explanation
Fig. 1 is the structural representation of simple incubator of the present invention.
Fig. 2 is the structural representation supporting screen 4 in Fig. 1.
Embodiment
As Fig. 1, shown in 2, simple incubator of the present invention, comprise casing, for making it with low cost and there is good heat insulation effect, this casing adopts the bubble chamber 2 with movable top lid 1, the upper level of bubble chamber 2 is provided with the number with four locating hole 3(locating holes 3 and can increases and decreases according to the actual requirements) support screen 4, movable top lid 1 offers four through holes 5 corresponding with locating hole 3 position, the blake bottle 7 being loaded with Drosophila medium 6 passes successively and extends to the bottom of bubble chamber 2 from the through hole 5 of movable top lid 1 and the locating hole 3 of support screen 4, the bottleneck of blake bottle 7 is higher than the upper surface of movable top lid 1, breathable insulation tampon 8 is provided with in bottleneck.
Above-mentioned simple incubator is used to make the pass the winter method in summer of fruit bat degree of safety be:
First prepare fresh Drosophila medium, and be divided in four blake bottles 7, making the height of medium 6 be about 1/3 of blake bottle 7 height can meet the demands, (wild-type Drosophila viability is strong to determine to place in each blake bottle the quantity of fruit bat according to the kind of fruit bat, 5 heros 5 can be put female in each blake bottle, the viability of saltant type fruit bat is more weak, suitably can increase the quantity of fruit bat, increase the probability that it is become pregnant), label outside blake bottle 7, indicate drosophila strains (the general one-tenth fly selecting about 3 ~ 20 ages in days that vitality is vigorous), the number of female male drosophila and blake bottle numbering, then blake bottle 7 is once placed in simple incubator, and fill heat-preservation cotton 9 by its complementary space in bubble chamber, finally close upper cover 1,
When winter, (winter vacation) used, this simple incubator can be placed on area without shade in room (as on the windowsill that faces south), under normal circumstances, temperature in room is all more than 10 DEG C, under the solar radiation of time-division at noon, temperature can reach more than 16 DEG C, can meet the cultivation breeding of fruit bat completely; Week about, take out the breeding situation that blake bottle 7 checks fruit bat, change fresh culture as required simultaneously;
When summer, (summer vacation) used, this simple incubator is placed on 4 DEG C of fridge freshness retaining indoor, week about, this simple incubator is taken out from refrigerator and to be placed on Indoor Natural environment one day, then take out the breeding situation that blake bottle checks fruit bat, then put into the indoor continuation cultivation of 4 DEG C of fridge freshness retainings, if incubator is more, for avoiding forgeing and obscure, a schedule planning table can be made; Owing to cultivating 4 DEG C of fridge freshness retaining indoor, the reproduction speed of fruit bat can obviously slow down, need not frequent replaced medium, and the reproduction speed of the fruit bat of different lines in different blake bottle is also different, generally, 3 ~ 4 weeks time as required configurable a collection of fresh culture carry out turning culture.
The inventive method is simple, can carry out in the family during winter vacation, and be beneficial to houshold refrigerator summer vacation or use laboratory to preserve the refrigerator of reagent, fund input is few, and cost is low, economic environmental protection.
Here utilizes intelligent climatic cabinate constant temperature culture, directly cultivates in laboratory without this simple incubator, cultivates and adopt simple incubator of the present invention and cultivate according to the inventive method the research conditions that four kinds of forms cultivate fruit bats without this simple incubator but according to the inventive method:
1. material:
Drosophila strains: wild-type Drosophila, is collected by field and obtains;
Reagent: agar, sucrose, propionic acid, dusty yeast, ether, it is pure that reagent is domestic analysis.
Instrument: simple incubator, electronic balance, domestic refrigerator, electric furnace, beaker etc.
2. method
The preparation of 2.1 medium
Medium is according to the proportions adding corn flour 11.2g, sucrose 8.9g, agar 1.0g, propionic acid 0.8mL, dried yeast powder 1.0g in every 100mL distilled water.
2.2 test groupings
Collect the fruit bat of about 3 ~ 20 ages in days, distinguish male and female, transfer in culture tube after etherization, often pipe 5 female 5 is male, and they are divided into 4 groups by preparation 40 culture tubes altogether, often organizes 10 pipes.4 groups are respectively intelligent climatic cabinate constant temperature culture (I group), in laboratory, directly cultivate (II group) without this simple incubator, cultivate (III group) and use simple incubator without simple incubator according to method of the present invention and according to method of the present invention cultivation (IV group).Spend the summer and the experiment of passing the winter all adopts identical test packet design.Crossing in summer test, III and IV group is before putting into refrigerator and when training all terminates and observes and data statistics growing of fruit bat, meanwhile, does same observation and statistics to I and II group.For comparative test effect, not replaced medium in incubation.
3. results and analysis
3.12014 the summer that crosses in summer vacation tests
I group, table 1 it's the summer (25 DEG C) utilizes to past intelligent climatic cabinate constant temperature culture
。
II group, table 2 directly cultivated the summer without simple incubator in laboratory
。
III group, table 3 is without simple incubator but the summer was cultivated in employing the inventive method (utilizing refrigerator)
。
IV group, table 4 adopts the inventive method (utilizing simple incubator and refrigerator) to cultivate the summer
。
From the result of table 1 ~ table 4, what growth rate was the fastest is II group, and be secondly I group, III group, the slowest is IV group.Although I group and II group are at first, fruit bat breeding is vigorous, and very fast along with the consumption of medium and the generation of pollution, fruit bat is mortality again, to last day, and I remaining 86 of composition fly, II group only remaining 5.Want slow with I group and II group of phase specific rate for III group, living contaminants is in confused situation aobvious, but its rate of development compared with IV group wants fast, because medium exhausts, become fly only surplus 181, and IV composition fly still has 306 to last day.Comprehensive above to think that IV group of best results, when not replaced medium, has been carried out conservation to fruit bat and bred.
3.22015 the winterization test in winter vacation
I group, table 5 utilizes intelligent climatic cabinate constant temperature culture to pass the winter (25 DEG C)
。
II group, table 6 is directly cultivated without simple incubator and is passed the winter in laboratory
。
III group, table 7 is without simple incubator but adopt the inventive method (on the sunny side) cultivation to pass the winter in the room in
。
IV group, table 8 adopts the inventive method (utilizing simple incubator area without shade in room) cultivation to pass the winter
。
From the result of table 5 ~ table 8, what growth rate was the fastest is I group, and be secondly IV group, III group, the slowest is II group.The fruit bat breeding of I group is vigorous, but exhausts along with medium, and death has appearred in fruit bat, and to last day, I composition fly number is 620.To compare rate of development with III group want fast with II group for IV group, to last day, IV composition fly number is 343, and II composition fly number is 235, and III composition fly number is 284.Although the one-tenth fly number of IV group is lower than I group, when do not use intelligent climatic cabinate and not replaced medium, IV group has successfully been carried out conservation to fruit bat and has bred.Comprehensively think, IV group of best results.
4. conclusion
As can be seen from the above-mentioned result of the test spent the summer, pass the winter, adopt simple incubator of the present invention and method to carry out cultivation and fruit bat can be assisted smoothly to spend the summer and pass the winter, the conservation achieving fruit bat is bred, and drop into little, cost is low, and method is easy, workload is little, is applicable to applying.
Claims (2)
1. a simple incubator, comprise casing, it is characterized in that: described casing is the bubble chamber (2) with movable top lid (1), the upper level of described bubble chamber (2) is provided with the support screen (4) with locating hole (3), described movable top lid (1) offers the through hole (5) corresponding with described locating hole (3) position, the blake bottle (7) being loaded with Drosophila medium (6) successively from the through hole of movable top lid (1) (5) and support screen (4) locating hole (3) pass and extend to the bottom of described bubble chamber (2), the bottleneck of described blake bottle (7) is higher than the upper surface of described movable top lid (1), breathable insulation tampon (8) is provided with in described bottleneck.
2. use the simple incubator in claim 1 that fruit bat degree of safety is passed the winter the method in summer, it is characterized in that: comprise the steps:
First prepare fresh Drosophila medium, and be divided in blake bottle, make the height of medium be about 1/3 of blake bottle height; The quantity of placing fruit bat in each blake bottle is determined according to the kind of fruit bat, label outside blake bottle, indicate number and the blake bottle numbering of drosophila strains, female male drosophila, then blake bottle is placed in above-mentioned simple incubator, and fill heat-preservation cotton by its complementary space in bubble chamber, close upper cover;
During use in winter, this simple incubator is placed on area without shade in room, week about, takes out the breeding situation that blake bottle checks fruit bat, change fresh culture as required simultaneously;
Summer is when using, this simple incubator is placed on 4 DEG C of fridge freshness retaining indoor, week about, this simple incubator is taken out from refrigerator and to be placed on Indoor Natural environment one day, then the breeding situation that blake bottle checks fruit bat is taken out, put into the indoor continuation of 4 DEG C of fridge freshness retainings again to cultivate, change fresh culture when 3 ~ 4 weeks as required and carry out turning culture.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716518.XA CN105393987B (en) | 2015-10-30 | 2015-10-30 | A kind of simple incubator and drosophila degree of safety is set to pass the winter the method in summer using the simple incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716518.XA CN105393987B (en) | 2015-10-30 | 2015-10-30 | A kind of simple incubator and drosophila degree of safety is set to pass the winter the method in summer using the simple incubator |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN105393987A true CN105393987A (en) | 2016-03-16 |
| CN105393987B CN105393987B (en) | 2018-01-02 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510716518.XA Expired - Fee Related CN105393987B (en) | 2015-10-30 | 2015-10-30 | A kind of simple incubator and drosophila degree of safety is set to pass the winter the method in summer using the simple incubator |
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| Country | Link |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019083744A1 (en) * | 2017-10-26 | 2019-05-02 | The Regents Of The University Of California | Drosophila stock maintenance |
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| DE10155631A1 (en) * | 2000-11-14 | 2002-07-04 | Arro Technics B V | Climate box consists of plastic profiles and plastic wall plates which take the form of a lidded shell whose principal surfaces are substantially parallel to one another |
| CN1810102A (en) * | 2005-12-09 | 2006-08-02 | 深圳出入境检验检疫局动植物检验检疫技术中心 | Fruit fly culturing process and culturing case |
| CN201025827Y (en) * | 2007-05-17 | 2008-02-27 | 中国林业科学研究院资源昆虫研究所 | Storehouse for storing butterfly pupa |
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| CN102669064A (en) * | 2012-06-26 | 2012-09-19 | 常熟南师大发展研究院有限公司 | Box type insect incubator |
| CN103238570A (en) * | 2013-05-15 | 2013-08-14 | 贵州大学 | Insect egg incubator |
| CN204259645U (en) * | 2014-11-07 | 2015-04-15 | 福建农林大学 | A kind of fruit bat beat exposure test Special artificial climate box |
| CN204377730U (en) * | 2014-12-19 | 2015-06-10 | 陈绩源 | A kind of drosophila larvae collects raising device |
-
2015
- 2015-10-30 CN CN201510716518.XA patent/CN105393987B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE10155631A1 (en) * | 2000-11-14 | 2002-07-04 | Arro Technics B V | Climate box consists of plastic profiles and plastic wall plates which take the form of a lidded shell whose principal surfaces are substantially parallel to one another |
| CN1810102A (en) * | 2005-12-09 | 2006-08-02 | 深圳出入境检验检疫局动植物检验检疫技术中心 | Fruit fly culturing process and culturing case |
| CN201025827Y (en) * | 2007-05-17 | 2008-02-27 | 中国林业科学研究院资源昆虫研究所 | Storehouse for storing butterfly pupa |
| CN201978751U (en) * | 2010-12-14 | 2011-09-21 | 陈鑫磊 | Heat preservation experiment box |
| CN102669064A (en) * | 2012-06-26 | 2012-09-19 | 常熟南师大发展研究院有限公司 | Box type insect incubator |
| CN103238570A (en) * | 2013-05-15 | 2013-08-14 | 贵州大学 | Insect egg incubator |
| CN204259645U (en) * | 2014-11-07 | 2015-04-15 | 福建农林大学 | A kind of fruit bat beat exposure test Special artificial climate box |
| CN204377730U (en) * | 2014-12-19 | 2015-06-10 | 陈绩源 | A kind of drosophila larvae collects raising device |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019083744A1 (en) * | 2017-10-26 | 2019-05-02 | The Regents Of The University Of California | Drosophila stock maintenance |
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| CN105393987B (en) | 2018-01-02 |
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Inventor after: Huang Yaqin Inventor after: Li Jinzhe Inventor after: Wang Ruili Inventor after: Liu Minjie Inventor after: Chen Yihui Inventor after: Wang Dezhi Inventor after: Ma Junyi Inventor before: Huang Yaqin Inventor before: Li Jinzhe Inventor before: Pang Ruihua |
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