CN105393987B - A kind of simple incubator and drosophila degree of safety is set to pass the winter the method in summer using the simple incubator - Google Patents
A kind of simple incubator and drosophila degree of safety is set to pass the winter the method in summer using the simple incubator Download PDFInfo
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- CN105393987B CN105393987B CN201510716518.XA CN201510716518A CN105393987B CN 105393987 B CN105393987 B CN 105393987B CN 201510716518 A CN201510716518 A CN 201510716518A CN 105393987 B CN105393987 B CN 105393987B
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- drosophila
- blake bottle
- incubator
- summer
- simple incubator
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
- A01K67/033—Rearing or breeding invertebrates; New breeds of invertebrates
Abstract
The invention discloses a kind of simple incubator, including the bubble chamber with upper lid, the top of bubble chamber is provided with support screen, through hole is offered on movable top lid, blake bottle is extended through to the bottom of bubble chamber from the positioning hole of the through hole of upper lid and support screen, the bottleneck of blake bottle is higher than upper lid, and tampon is provided with bottleneck.Winter places it in area without shade in room, week about, checks the breeding situation of drosophila, while change fresh culture as needed；Summer is placed it in 4 DEG C of fridge freshness retaining rooms, week about, is drawn off being placed in indoor environment one day, is then checked the breeding situation of drosophila, is placed into refrigerator and continue to cultivate, change as needed at 3 ~ 4 weeks fresh culture turn being commissioned to train it is foster.The present invention uses cheap bubble chamber, and as drosophila incubator, simple in construction, manufacturing cost is cheap, in use, can ensure the safety breeding of drosophila population during winter vacation and summer vacation, meets the demand that drosophila conservation is bred.
Description
Technical field
The present invention relates to science of heredity and Developmental Biology technology, and more particularly, to a kind of simple incubator, the present invention also relates to
And drosophila degree of safety is set to pass the winter the method in summer using the simple incubator.
Background technology
Drosophila melanogaster (Drosophila melanogaster), Insecta, Diptera, Drosophilidae, it is that the current mankind grind
Most thoroughly one of the biology studied carefully, itself have the characteristics that breeding is fast, the history of life is short, filial generation number is more, and gene sequencing is complete
Into being important genetics research model organism, there is consequence in Genetics Experimental Teaching and scientific research.Using drosophila as
Need to breed drosophila progress conservation in the scientific research and education activities of object, especially some rare mutant lines will more be protected
Procreation can be expanded by demonstrate,proving it.Drosophila is very sensitive to ambient temperature, and when temperature is less than 5 DEG C, drosophila can not move at all, and the time has been grown just
Can be dead because hungry；When temperature is higher than 30 DEG C, infertility or even death occurs in drosophila.Generally, researcher is by fruit
Fly, which is placed in culture medium in 25 DEG C of intelligent growth cabinets, expands culture, and a subculture is changed every 7 or so.
Because intelligent growth cabinet is expensive, long-time work double tides need to consume substantial amounts of electric energy, and toxigenic capacity is high,
And noise is produced during incubator work, polluted the environment during compressor cooling.And under normal circumstances, summer and winter are all
Peak times of power consumption, cooling and heating will consume the most electric power in China, intelligent growth cabinet at this moment also often because
It can not start for brownout, influence normal training.
The researcher of institution of higher education has two vacations of winter vacation and summer vacation every year, carrys out interim, religion when winter vacation and summer vacation
And research work will come to an end.If daily 24 hours of intelligent growth cabinet are allowed with the breeding of drosophila only for conservation
Work, toxigenic capacity are higher；Also need to configure a collection of fresh culture medium every 7 or so, also add work to researcher
Measure.Therefore, it is necessary to design it is a kind of drosophila degree of safety can be made to pass the winter the Simple culture method and culture apparatus in summer, drosophila can be given
The culture environment of one constant temperature is provided, ensures the procreation of drosophila population, also wants low-carbon economy, simple to operate.
The content of the invention
The defects of it is an object of the invention to exist for prior art and a kind of simple incubator is provided, the present invention also carry
For making drosophila degree of safety pass the winter the method in summer using the simple incubator.
To achieve the above object, the present invention can take following technical proposals：
Simple incubator of the present invention, including casing, the casing are the bubble chamber with movable top lid, the bubble
The upper level of foam case is provided with the support screen with positioning hole, is offered on the movable top lid and the position of positioning hole
Corresponding through hole, the blake bottle of Drosophila medium is loaded with successively from the positioning hole of the through hole of movable top lid and support screen
Extend through to the bottom of the bubble chamber, the bottleneck of the blake bottle is higher than the upper surface of the movable top lid, described
Breathable insulation tampon is provided with bottleneck.
Comprise the steps the pass the winter method in summer of drosophila degree of safety using the simple incubator of the present invention：
Fresh Drosophila medium is prepared first, and is divided in blake bottle, the height of culture medium is about cultivated
The 1/3 of bottle height degree；Determined to place the quantity of drosophila in each blake bottle according to the kind of drosophila, label, mark outside blake bottle
Blake bottle, is then placed in above-mentioned simple incubator by bright drosophila strains, the number of female male drosophila and blake bottle numbering, and will bubble
Heat-preservation cotton is filled in its complementary space in foam case, closes lid；
During use in winter, the simple incubator is placed on area without shade in room, week about, blake bottle is taken out and checks drosophila
Breeding situation, while change fresh culture as needed；
Summer is in use, the simple incubator is placed in 4 DEG C of fridge freshness retaining rooms, week about, by the simple incubator
Take out and be placed in Indoor Natural environment one day from refrigerator, then take out the breeding situation that blake bottle checks drosophila, place into 4 DEG C of ice
Continue to cultivate in case fresh-keeping chamber, change as needed at 3 ~ 4 weeks fresh culture turn being commissioned to train it is foster.
The advantage of the invention is that using the heat preservation and insulation of cystosepiment, drosophila is used as using cheap bubble chamber
Incubator, simple in construction, manufacturing cost is cheap, and bubble chamber expands numerous provide effectively with the heat-preservation cotton filled in it to drosophila
Thermal environment, beneficial to preserving and expand numerous drosophila species；In use, the safety of drosophila population can be ensured during winter vacation and summer vacation
Breeding, meets the demand that drosophila conservation is bred.
Brief description of the drawings
Fig. 1 is the structural representation of simple incubator of the present invention.
Fig. 2 is the structural representation that screen 4 is supported in Fig. 1.
Embodiment
As shown in Figure 1, 2, simple incubator of the present invention, including casing, it is preferable to make its cost cheap and having
Heat insulation effect, the casing uses the bubble chamber 2 with movable top lid 1, and the upper level of bubble chamber 2 is provided with fixed with four
Position hole 3（The number of positioning hole 3 can increase and decrease according to the actual requirements）Support screen 4, offer and positioning hole 3 on movable top lid 1
Four corresponding through holes 5 of position, it is loaded with the through hole 5 from movable top lid 1 and the support successively of blake bottle 7 of Drosophila medium 6
Being extended through in the positioning hole 3 of screen 4 to the bottom of bubble chamber 2, the bottleneck of blake bottle 7 is higher than the upper surface of movable top lid 1,
Breathable insulation tampon 8 is provided with bottleneck.
The pass the winter method in summer of drosophila degree of safety is set to be using above-mentioned simple incubator：
Fresh Drosophila medium is prepared first, and is divided in four blake bottles 7, makes the height of culture medium 6 about
It can meet to require for the 1/3 of the height of blake bottle 7；Determined to place the quantity of drosophila in each blake bottle according to the kind of drosophila
（Wild-type Drosophila viability is strong, can put that 5 heros are 5 female, and the viability of saltant type drosophila is weaker, can be appropriate in each blake bottle
Increase the quantity of drosophila, increase its probability become pregnant）, labelled outside blake bottle 7, indicate drosophila strains（It is typically chosen life
The adult flies of 3 ~ 20 or so vigorous ages in days of power）, female male drosophila number and blake bottle numbering, then blake bottle 7 is once placed
In simple incubator, and heat-preservation cotton 9 will be filled in its complementary space in bubble chamber, finally close lid 1；
Winter（Winter vacation）In use, the simple incubator can be placed on to area without shade in room（On windowsill on the sunny side）, just
In the case of often, the temperature in room is more than 10 DEG C, and at noon under the sunlight of time-division, temperature, can up to more than 16 DEG C
Fully meet the culture breeding of drosophila；Week about, the breeding situation that blake bottle 7 checks drosophila is taken out, while as needed more
Change fresh culture；
Summer（Summer vacation）In use, the simple incubator is placed in 4 DEG C of fridge freshness retaining rooms, it is week about, this is simple
Incubator takes out from refrigerator and is placed in Indoor Natural environment one day, then takes out blake bottle and checks the breeding situation of drosophila, then puts
Enter and continue to cultivate in 4 DEG C of fridge freshness retaining rooms, if incubator is more, to avoid forgeing and obscuring, a schedule planning table can be made；
Due to being cultivated in 4 DEG C of fridge freshness retaining rooms, the reproduction speed of drosophila can substantially slow down, without frequently changing culture medium, and
Reproduction speed of the drosophila of different lines in different blake bottles is also different, generally, at 3 ~ 4 weeks can configure as needed
A collection of fresh culture carry out turn be commissioned to train it is foster.
The inventive method is simple, can be carried out in the family during winter vacation, and summer vacation is beneficial to houshold refrigerator or uses laboratory
The refrigerator of reagent is preserved, fund input is few, and cost is low, economic and environment-friendly.
Here be using intelligent growth cabinet is incubated, cultivated without the simple incubator directly in laboratory,
And without the simple incubator but according to the inventive method culture train using simple incubator of the present invention and according to the inventive method
Support the research conditions of four kinds of form culture drosophilas：
1. material：
Drosophila strains：Wild-type Drosophila, collected by field and obtained；
Reagent：Agar, sucrose, propionic acid, dusty yeast, ether, reagent are that domestic analysis is pure.
Instrument：Simple incubator, electronic balance, domestic refrigerator, electric furnace, beaker etc..
2. method
The preparation of 2.1 culture mediums
Culture medium according to added in every 100mL distilled water corn flour 11.2g, sucrose 8.9g, agar 1.0g, propionic acid 0.8mL,
Dried yeast powder 1.0g ratio is prepared.
2.2 experiment packets
The drosophila of 3 ~ 20 or so ages in days is collected, male and female is distinguished after etherization, is transferred in culture tube, often pipe 5 female 5 is male,
Prepare 40 culture tubes altogether, be classified into 4 groups, every group 10 is managed.4 groups are respectively intelligent growth cabinet incubated (I
Group), without the simple incubator cultivate directly in laboratory (II group), trained without simple incubator according to the method for the present invention
Support (III group) and using simple incubator and according to the method culture of the present invention（IV group）.The experiment spent the summer and passed the winter uses phase
Same experiment packet design.In summer experiment is crossed, III and IV group is before being put into refrigerator and when training all terminates
Growing for drosophila is observed and data statistics, at the same time, same observation and statistics are done to I and II group.For than
Compared with test effect, culture medium is changed without in incubation.
3. result and analysis
3.1 2014 summer vacations crossed summer experiment
1 I groups of table spends the summer using intelligent growth cabinet is incubated（25℃）
。
2 II groups of table directly cultivated the summer without simple incubator in laboratory
。
3 III groups of table does not have to simple incubator but uses the inventive method（Utilize refrigerator）Cultivated the summer
。
4 IV groups of table uses the inventive method（Utilize simple incubator and refrigerator）Cultivated the summer
。
From the result of 1~table of table 4, growth rate it is most fast be II group, next to that I group, III group, most slow is
IV group.Although I group and II group is at first, drosophila breeding it is vigorous, quickly with culture medium consumption and pollution production
Raw, drosophila mortality again, to last day, I composition fly is remaining 86, and II group only remaining 5.III group and I group and II group
Slow compared to speed, living contaminants is in confused situation aobvious, but its rate of development compared with IV group is fast, because culture medium has consumed
To the greatest extent, it is only surplus 181 to last day adult flies, and IV composition fly still has 306.In summary think, IV group of effect is most
It is good, in the case where being changed without culture medium, conservation is carried out to drosophila and bred.
The winterization test in 3.2 2015 winter vacations
5 I groups of table is passed the winter using intelligent growth cabinet is incubated（25℃）
。
6 II groups of table is cultivated without simple incubator directly in laboratory passes the winter
。
7 III groups of table does not have to simple incubator but uses the inventive method（In room on the sunny side）Culture is passed the winter
。
8 IV groups of table uses the inventive method（Using simple incubator in room area without shade）Culture is passed the winter
。
From the result of 5~table of table 8, growth rate it is most fast be I group, next to that IV group, III group, most slow is
II group.I group of drosophila breeding is vigorous, but as culture medium exhausts, death occurs in drosophila, to last day, I composition fly
Number is 620.IV group rate of development will soon compared with II group and III group, and to last day, IV composition fly number is 343, and
II composition fly number is 235, and III composition fly number is 284.Although IV group of adult flies number is less than I group, without using intelligence
Growth cabinet and in the case of being changed without culture medium, IV group has successfully carried out conservation to drosophila and has bred.Comprehensive analysis thinks,
IV group of best results.
4. conclusion
It can be seen that from the above-mentioned result of the test spent the summer, passed the winter and cultivated using simple incubator of the present invention and method
It can smoothly aid in drosophila to cross the summer and to pass the winter, the conservation for realizing drosophila is bred, and puts into small, and cost is low, and method is easy, work
Work amount is small, is adapted to popularization and application.
Claims (1)
1. drosophila degree of safety is set to pass the winter the method in summer using simple incubator, it is characterised in that：The simple incubator of use includes
Casing, the casing are with movable top lid（1）Bubble chamber（2）, the bubble chamber（2）Upper level be provided with fixed
Position hole（3）Support screen（4）, the movable top lid（1）On offer and the positioning hole（3）The corresponding through hole in position
（5）, it is loaded with Drosophila medium（6）Blake bottle（7）Successively from movable top lid（1）Through hole（5）With support screen（4）Determine
Position hole（3）In extend through to the bubble chamber（2）Bottom, the blake bottle（7）Bottleneck be higher than the movable top lid
（1）Upper surface, breathable insulation tampon is provided with the bottleneck（8）；
Specifically incubation step is：
Fresh Drosophila medium is prepared first, and is divided in blake bottle, the height of culture medium is about cultivated bottle height
The 1/3 of degree；Determined to place the quantity of drosophila in each blake bottle according to the kind of drosophila, labelled outside blake bottle, indicate fruit
Blake bottle, is then placed in above-mentioned simple incubator by fly strain, the number of female male drosophila and blake bottle numbering, and by bubble chamber
Heat-preservation cotton is filled in its interior complementary space, closes lid；
During use in winter, the simple incubator is placed on area without shade in room, week about, blake bottle is taken out and checks the numerous of drosophila
Situation is grown, while changes fresh culture as needed；
Summer in use, the simple incubator is placed in 4 DEG C of fridge freshness retaining rooms, week about, by the simple incubator from ice
Case takes out and is placed in Indoor Natural environment one day, then takes out the breeding situation that blake bottle checks drosophila, places into 4 DEG C of refrigerators and protects
Fresh interior continues to cultivate, change as needed at 3 ~ 4 weeks fresh culture turn being commissioned to train it is foster.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716518.XA CN105393987B (en) | 2015-10-30 | 2015-10-30 | A kind of simple incubator and drosophila degree of safety is set to pass the winter the method in summer using the simple incubator |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510716518.XA CN105393987B (en) | 2015-10-30 | 2015-10-30 | A kind of simple incubator and drosophila degree of safety is set to pass the winter the method in summer using the simple incubator |
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| Publication Number | Publication Date |
|---|---|
| CN105393987A CN105393987A (en) | 2016-03-16 |
| CN105393987B true CN105393987B (en) | 2018-01-02 |
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| CN201510716518.XA Expired - Fee Related CN105393987B (en) | 2015-10-30 | 2015-10-30 | A kind of simple incubator and drosophila degree of safety is set to pass the winter the method in summer using the simple incubator |
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| WO2019083744A1 (en) * | 2017-10-26 | 2019-05-02 | The Regents Of The University Of California | Drosophila stock maintenance |
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| NL1016602C2 (en) * | 2000-11-14 | 2002-05-16 | Arro Technics B V | Air handling unit. |
| CN100469241C (en) * | 2005-12-09 | 2009-03-18 | 深圳出入境检验检疫局动植物检验检疫技术中心 | Fruit fly culturing process and culturing case |
| CN201025827Y (en) * | 2007-05-17 | 2008-02-27 | 中国林业科学研究院资源昆虫研究所 | Storehouse for storing butterfly pupa |
| CN201978751U (en) * | 2010-12-14 | 2011-09-21 | 陈鑫磊 | Heat preservation experiment box |
| CN102669064A (en) * | 2012-06-26 | 2012-09-19 | 常熟南师大发展研究院有限公司 | Box type insect incubator |
| CN103238570A (en) * | 2013-05-15 | 2013-08-14 | 贵州大学 | Insect egg incubator |
| CN204259645U (en) * | 2014-11-07 | 2015-04-15 | 福建农林大学 | A kind of fruit bat beat exposure test Special artificial climate box |
| CN204377730U (en) * | 2014-12-19 | 2015-06-10 | 陈绩源 | A kind of drosophila larvae collects raising device |
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| CB03 | Change of inventor or designer information | ||
| CB03 | Change of inventor or designer information |
Inventor after: Huang Yaqin Inventor after: Li Jinzhe Inventor after: Wang Ruili Inventor after: Liu Minjie Inventor after: Chen Yihui Inventor after: Wang Dezhi Inventor after: Ma Junyi Inventor before: Huang Yaqin Inventor before: Li Jinzhe Inventor before: Pang Ruihua |
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| TA01 | Transfer of patent application right | ||
| TA01 | Transfer of patent application right |
Effective date of registration: 20170721 Address after: 464000 College of biological and pharmaceutical engineering, Xinyang agriculture and Forestry College, 49 new twenty-four Avenue, Yangshan New District, Henan, Xinyang Applicant after: Xinyang College of Agriculture and Forestry Address before: 464000 College of biological and pharmaceutical engineering, Xinyang agriculture and Forestry College, 49 new twenty-four Avenue, Yangshan New District, Henan, Xinyang Applicant before: Huang Yaqin |
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Granted publication date: 20180102 Termination date: 20181030 |