CN1771790A - Breeding method for western pumpkin hybrid - Google Patents
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- CN1771790A CN1771790A CN 200510095182 CN200510095182A CN1771790A CN 1771790 A CN1771790 A CN 1771790A CN 200510095182 CN200510095182 CN 200510095182 CN 200510095182 A CN200510095182 A CN 200510095182A CN 1771790 A CN1771790 A CN 1771790A
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Abstract
The present invention relates to plant breeding method, and is especially breeding method of Western pumpkin hybrid. The basic steps include: 1. hybridizing with Western pumpkin high-generation selfing line 0326 as female parent and Chinese pumpkin high-generation selfing line 2017 as male parent to cultivate Western pumpkin high-generation selfing line 184-1; and 2. compounding Western pumpkin hybrid, named Ruilu-1, with Western pumpkin high-generation selfing line 3027 as female parent and Western pumpkin high-generation selfing line 184-1 as male parent. Thus cultivated new pumpkin hybrid has high heat resistance, high disease resistance, high quality, high yield and other outstanding advantages.
Description
Technical field
The present invention relates to a kind of breeding method of hybrid plant, especially a kind of breeding method of western pumpkin hybrid.
Background technology
Cucurbita (Cucurbita) has three main cultispecies, i.e. american pumpkin (C.pepo L), China squash (C.moschata D.) and giant pumpkin (C.maxima D.).Western pumpkin claims to tremble pumpkin again, belongs to the giant pumpkin cultivated species, and nutritious because of it, quality and flavor is splendid, is subjected to producers and consumers's welcome deeply, and area under cultivation enlarges year by year in recent years, but majority is an introduced variety.The general non-refractory of this class pumpkin, virus disease takes place seriously during the broiling summer, and spring, late sowing or autumn culture often caused total crop failure, had therefore limited extensive plantation.And China squash has heat-resisting, disease-resistant, adaptable characteristics, but taste quality can not show a candle to western pumpkin.
The applying date is that 1992.12.03, application number are 92113528, are awarded Patent right Chinese invention patent application and disclose a kind of short living China squash cross-breeding method, this method is selected the short living China squash inbred line and the pumpkin variety of overgrowing of assembly mutually with it, with short living inbred line 84 A1-0-4* is female parent, short southern No. 1 with making as local early pumpkin kind 5-1* (the seven leaf melons) hybridization of male parent (0-4 * 5-1).
But do not see so far by hybridization, the pumpkin new varieties that make western pumpkin combine and cultivate with the China squash advantage.
Summary of the invention
The objective of the invention is to: the shortcoming at above-mentioned existing western pumpkin kind exists, propose a kind of breeding method of western pumpkin hybrid, thereby cultivate heat-resisting, disease-resistant, adaptable western pumpkin hybrid, make it can obtain the large tracts of land popularizing planting.
In order to reach above purpose, the applicant finds that the breeding method of following western pumpkin hybrid not only can well reach goal of the invention, and has beyond thought effect that through repetition test for many years its basic step is in many hybridization cultivation schemes:
(1) being female parent with the western pumpkin height for inbred line 0326, is that male parent is hybridized with the China squash height for inbred line 2017, cultivates the western pumpkin height for inbred line 184-1;
(2) being female parent with the western pumpkin height for inbred line 3027, is male parent with the western pumpkin height for inbred line 184-1, makes western pumpkin crossbreed (called after: " auspicious green No. 1 ").
Above method comes down to heat-resisting by selecting, disease-resistant material, and sexual hybridization on good inbred line, can be bred 2 generations in common 1 year with its disease-resistant gene transformation, prepares combination for behind (more than five generations) selfing purifying as the parent through many.
Facts have proved that the pumpkin new varieties that the present invention cultivates have following outstanding advantage:
1, good heat resistance.The western pumpkin crossbreed that the present invention breeds can be under 32～35 ℃ high temperature well-grown, and main at present western pumpkin kind of planting " east rises " undergrowth.
2, disease resistance is strong.Auspicious green No. 1 high antiviral disease, the disease index that normal time east rises is 23.56～25.16, and auspicious green No. 1 disease index only is 5.12～6.82, its powdery mildew is also light than " east rises ".
3, product are of fine quality.Melon skin is dark green, and melon meat is orange, quality powder, glutinous, sweet, and adaptability is good, liked by the consumer.
4, output is higher.Spring cultivation yield increased east is peaceful all improves 97.6%.Western pumpkin high temperature season virus takes place seriously in the past, can not plant autumn substantially, even and the kind autumn culture that the present invention cultivates also obtains higher output, obtained beyond thought good harvest.
Description of drawings
Fig. 1 is the seed selection spectrum schematic diagram of the embodiment of the invention.
Embodiment
Below in conjunction with specific embodiment the present invention is described in further details.
Embodiment
Concrete enforcement of the present invention is as follows:
One, parental source and feature thereof
The western pumpkin kind of seed selection high antiviral disease, it is crucial screening anti-source.The applicant from domestic and international introduced 40 surplus part western pumpkin variety source, all do not screen the viral diseases material, turn to China squash so will seek the sight in anti-source.
Tanaka in suburb, Zhenjiang farmers' finds curcumbitate and the similar China squash of western pumpkin, and disease resistance is very strong, with being about to its selfing purifying, breeds 2017 stable strains through the selfing of 3 generations.The characteristics that though 2017 strains have is heat-resisting, high antiviral is sick, inferior quality.With 2017 is male parent with product are of fine quality, coordinate force is strong western pumpkin height for inbred line 0326 hybridization, with the target selection of filial generation by heat-resisting, disease-resistant and high-quality, by must the western pumpkin height after 5 generations of selfing for inbred line 184-1.
High feature for inbred line 184-1: ripe early, growth potential is strong, and blade is dark green, and fruit is oblate, the pericarp bottle green, and carpopodium is short and thick, and the pulp orange colour is more than the thick 3cm of meat, about the heavy 1.5kg of single melon.First female flower joint position, 10～12 joints later on every 3 joint regeneration, one joint female flower, but also have the consecutive numbers joint that the female flower phenomenon takes place.This strain biggest advantage be have heat-resisting, high antiviral is sick, more anti-powdery mildew is simultaneously than the characteristic of high-quality.
High obtain its feature from 4 generations of western pumpkin " east rises " selfing for inbred line 3027: precocity, growth potential is medium, and blade is dark green, fruit is oblate, and the melon skin bottle green is the light green color decorative pattern slightly, the pulp orange colour, and meat is fine and closely woven, sweet of mouthfeel, best in quality, single melon weighs 1～1.5kg.First female flower joint position is at 9～11 joints.But disease resistance is general, and its disease tolerance is slightly stronger than " east rises ".
Two, preparing hybrid kind " auspicious green No. 1 "
Is female parent with height for inbred line 3027, and high to be that male parent is mixed with heat-resisting, disease-resistant, the crossbreed " auspicious green No. 1 " of high-quality, precocity, high yield for inbred line 184-1, and concrete seed selection spectrum as shown in Figure 1.
The feature of " auspicious green No. 1 " is:
Precocity, the plant strain growth gesture is strong, and blade is big and dark green, and is heat-resisting, high antiviral is sick, more anti-powdery mildew, fruit be oblate, bottle green, the pulp orange colour, more than the thick 3cm of meat, about the heavy 2kg of single melon, product are of fine quality, suitable spring, autumn two season cultivation.(referring to table 1)
Should note following aspect in the above culturing cenospecies production process:
1, isolation distance: more than other pumpkin variety isolation distance of hybrid seeding Tian Yaoyu 1000m.
2, adjust this flower synchronization of father and mother: male parent is than maternal planting early enough 7-10 days, to improve the flower synchronization probability.
3, this planting proportion of father and mother: for improving the yield per unit area of first generation of hybrid seed, father and mother's row originally is than disposing by 1: 4.Spacing in the rows 40 ± 5cm, line-spacing is decided with booth, guarantees every 667m
2Plant 600 ± 50 strains.
4, producing method for seed: in flowering stage, every other day the male flower on the female parent is removed, rely on insect pollination such as honeybee.Be the raising purity of hybrid, but also Artificial Control hybridization.Its method is: pollination noon before that day selects maternal female flower and the male parent male flower that can bloom in second day, corolla is clamped or with on the bundle of lines bud front end bundle, made insect advance not go with instruments such as a pod or elongate thin aluminum strips.Pollinate during the next morning 7-9, the male parent stamen pollen is fully smeared on maternal column cap, and then female flower is pricked, and list and make marks.
5, hybridization back plant management: after the week of pollinating, in time non-hybridization melon and melon profile on the female parent and the discrepant melon of maternal standard proterties are removed.
6, collect seed: general female flower back about the 55 days results of blooming, plant and will be placed on shady and cool place's after-ripening 15 ± 2 days after melon is adopted, seed can be taken out to clean and dry, be placed on low temperature, drying condition is preserved down.General every 667m
2Can produce hybrid seed 40kg.
The auspicious green comparative result that rises with east for No. 1 of table 1
| Time | Kind | The virus state of an illness | Output (kg/667m 2) |
| 2003 | Auspicious green No. 1 | 6.82 | 1924 |
| East rises | 25.16 | 876 | |
| Improve (%) | 72.9 | 119.6 | |
| 2004 | Auspicious green No. 1 | 5.12 | 2218 |
| East rises | 23.56 | 1264 | |
| Improve (%) | 78.3 | 75.5 | |
| The average improvement | 75.6 | 97.6 |
Conclusion is got up, and the cultivating process of present embodiment can be sketched and be:
(1) the western pumpkin height with high-quality is a female parent for inbred line 0326, and the China squash height extremely strong with disease resistance is that male parent is hybridized for inbred line 2017, and the western pumpkin height of breeding disease-resistant high-quality is for inbred line 184-1.
(2) the western pumpkin height with high-quality is a female parent for inbred line 3027, and the western pumpkin height of disease-resistant high-quality is a male parent for inbred line 184-1, preparation western pumpkin crossbreed: " auspicious green No. 1 ".
Many outstanding advantages such as that evidence, the pumpkin new varieties of cultivating by the present embodiment method have is heat-resisting, disease-resistant, high-quality, high yield, plantation suits large area to popularize.
Claims (9)
1. the breeding method of a western pumpkin hybrid, basic step is:
(1) being female parent with the western pumpkin height for inbred line 0326, is that male parent is hybridized with the China squash height for inbred line 2017, cultivates the western pumpkin height for inbred line 184-1;
(2) being female parent with the western pumpkin height for inbred line 3027, is male parent with the western pumpkin height for inbred line 184-1, makes the western pumpkin crossbreed.
2. according to the breeding method of the described western pumpkin hybrid of claim 1, it is characterized in that: in the described step (1), it is male parent that China squash is bred 2017 stable product through the above selfing of 3 generations.
3. according to the breeding method of the described western pumpkin hybrid of claim 2, it is characterized in that: in the described step (2), western pumpkin is bred stable 184-1 product after the above selfing of 5 generations be male parent.
4. according to the breeding method of the described western pumpkin hybrid of claim 3, it is characterized in that: hybrid seeding field and more than other pumpkin variety isolation distance 1000m.
5. according to the breeding method of the described western pumpkin hybrid of claim 3, it is characterized in that: described male parent was than maternal planting early enough 7-10 days.
6. according to the breeding method of the described western pumpkin hybrid of claim 5, it is characterized in that: described this planting proportion of father and mother father and mother row originally is than disposing spacing in the rows 40 ± 5cm, every 667m by 1: 4
2Plant 600 ± 50 strains.
7. according to the breeding method of the described western pumpkin hybrid of claim 6, it is characterized in that: in flowering stage, every other day the male flower on the female parent is removed, rely on insect pollination.
8. according to the breeding method of the described western pumpkin hybrid of claim 6, it is characterized in that: pollinate noon before that day, select maternal female flower and the male parent male flower that to bloom in second day, corolla is clamped or pricked with bundle of lines bud front end, pollinate during the next morning 7-9, the male parent stamen pollen is fully smeared on maternal column cap, and then female flower is pricked.
9. according to the breeding method of claim 7 or 8 described western pumpkin hybrids, it is characterized in that: after the week of pollinating, in time non-hybridization melon and melon profile on the female parent and the discrepant melon of maternal standard proterties are removed.
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| CNB2005100951826A CN100399881C (en) | 2005-11-02 | 2005-11-02 | Breeding method for western pumpkin hybrid |
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| CNB2005100951826A CN100399881C (en) | 2005-11-02 | 2005-11-02 | Breeding method for western pumpkin hybrid |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461101A (en) * | 2013-09-05 | 2013-12-25 | 镇江瑞繁农艺有限公司 | Stemless cucurbita maxima breeding method |
| CN107771669A (en) * | 2017-11-17 | 2018-03-09 | 衢州市农业科学研究院 | A kind of method of Cucurbita interspecific hybridization |
| CN112655547A (en) * | 2020-12-10 | 2021-04-16 | 哈尔滨市农业科学院 | Pumpkin breeding hybrid pollination method |
| CN113785770A (en) * | 2021-09-27 | 2021-12-14 | 禄劝普林生物科技有限责任公司 | Breeding method of high-quality small pumpkin inbred line |
| CN114391473A (en) * | 2022-02-15 | 2022-04-26 | 湖南省蔬菜研究所 | Pumpkin inbred line creation method with white flower mark and excellent comprehensive characters |
| WO2022196801A1 (en) * | 2021-03-18 | 2022-09-22 | 株式会社サカタのタネ | Novel pumpkin plant and method for producing same |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1029362C (en) * | 1992-12-03 | 1995-07-26 | 王甲生 | Method for hybridization of bush Chinese cushaw |
| CN1141868C (en) * | 2000-11-27 | 2004-03-17 | 王甲生 | Breeding method for gellow stem Chinese pumpkin |
-
2005
- 2005-11-02 CN CNB2005100951826A patent/CN100399881C/en not_active Expired - Fee Related
Cited By (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103461101A (en) * | 2013-09-05 | 2013-12-25 | 镇江瑞繁农艺有限公司 | Stemless cucurbita maxima breeding method |
| CN103461101B (en) * | 2013-09-05 | 2015-06-17 | 镇江瑞繁农艺有限公司 | Stemless cucurbita maxima breeding method |
| CN107771669A (en) * | 2017-11-17 | 2018-03-09 | 衢州市农业科学研究院 | A kind of method of Cucurbita interspecific hybridization |
| CN112655547A (en) * | 2020-12-10 | 2021-04-16 | 哈尔滨市农业科学院 | Pumpkin breeding hybrid pollination method |
| JP7214929B1 (en) * | 2021-03-18 | 2023-01-30 | 株式会社サカタのタネ | Novel pumpkin plant and method for producing the same |
| WO2022196801A1 (en) * | 2021-03-18 | 2022-09-22 | 株式会社サカタのタネ | Novel pumpkin plant and method for producing same |
| KR20220131329A (en) * | 2021-03-18 | 2022-09-27 | 가부시키가이샤 사카타노타네 | Novel pumpkin plant and manufacturing method thereof |
| CN115666226A (en) * | 2021-03-18 | 2023-01-31 | 坂田种苗株式会社 | Novel pumpkin plant and method for producing the same |
| KR102595279B1 (en) | 2021-03-18 | 2023-10-27 | 가부시키가이샤 사카타노타네 | New pumpkin plants and methods for producing them |
| CN113785770B (en) * | 2021-09-27 | 2022-07-29 | 禄劝普林生物科技有限责任公司 | Breeding method of high-quality small pumpkin inbred line |
| CN113785770A (en) * | 2021-09-27 | 2021-12-14 | 禄劝普林生物科技有限责任公司 | Breeding method of high-quality small pumpkin inbred line |
| CN114391473A (en) * | 2022-02-15 | 2022-04-26 | 湖南省蔬菜研究所 | Pumpkin inbred line creation method with white flower mark and excellent comprehensive characters |
| CN114391473B (en) * | 2022-02-15 | 2023-06-30 | 湖南省蔬菜研究所 | Pumpkin inbred line creation method with white flower marks and excellent comprehensive properties |
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| CN100399881C (en) | 2008-07-09 |
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