CN1762460A

CN1762460A - The Chinese herbal medicine compound prescription of control seawater fish shield balantidiasis

Info

Publication number: CN1762460A
Application number: CNA2005100447552A
Authority: CN
Inventors: 王印庚; 陈洁君; 陈霞
Original assignee: Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Current assignee: Yellow Sea Fisheries Research Institute Chinese Academy of Fishery Sciences
Priority date: 2005-09-23
Filing date: 2005-09-23
Publication date: 2006-04-26
Anticipated expiration: 2025-09-23
Also published as: CN100512843C

Abstract

A kind of Chinese herbal medicine compound prescription of preventing and treating seawater fish shield balantidiasis, a kind of be used to propagating the aquatic products specific complex Chinese herb medicine that prevents under the condition and treat seawater fish shield infusorian disease artificially, its drug ingedient and proportioning (by weight score) are: Verbena officinalis 40%, hardship remonstrate with 30%, the root of large-flowered skullcap 30%, pulverizing is sieved through 100 orders, mixes to form. This prescription can be effectively be used for the young postlarva of various marine fish and prevention and the treatment of marketable fish mature stage Paralembus digitiformis disease, can be by the bait carrier oral or dipping mode use. This prescription belongs to available medicine, meet food security and nuisanceless requirement, its growth to seawater fish of clinical trial certificate has no side effect, using method is simple and convenient, consumption is little, drug effect is good, with low cost, be suitable for using under the various seawater fish intensive culture conditions, have widely penetration and promotion meaning, to improving cultivation survival rate and economic benefit, effectively reduce and prevent that the abuse of formaldehyde has great importance.

Description

The Chinese herbal medicine compound prescription of control seawater fish shield balantidiasis

Affiliated technical field

The invention belongs to the aquatic products specific complex Chinese herb medicine for the treatment of seawater fish shield infusorian disease, is to propagate artificially under the condition the young postlarva of seawater fish and forming method for preparation of drug and the application process thereof that fish shield balantidiasis prevents and treats.

Background technology

The protozoa species that parasitizes the tissues such as marine products economic fish body surface, the gill filament, internal organ and organ is a lot, and some kind can cause the serious disease of seawater fish. Along with flourish in the whole world of marine fish culture industry, since implementing particularly that high density is intensive and propagating artificially, the fish disease that these kinds cause is on the rise, and also can cause huge economic loss. Yet, countries in the world bias toward viral to the research of marine fish disease and bacteriosis on, and less to the research of protozoan disease, data is scattered, the research of control medicine is deficient especially. From calendar year 2001, we have carried out sick investigation and the Study on etiology learned of disease popularity of system to Coastal Area In Shandong Province main fish culture zone, find the parasitic protozoa class disease that several cause a disease seawater fish, for example Paralembus digitiformis disease (Scuticociliatosis), flagellosis (Giardiasis), cryptocaryoniosis (Cryptocaryoniosis) and trichodinasis (Trichodiniasis). Wherein the highest with the Paralembus digitiformis disease incidence, the harm that causes is maximum, has brought more serious economic loss for the cultivation dealer, so be widely regarded as (the Bo-Young et al. of one of most important disease in the industrial aquaculture fish, 2001, Iglesias R et al.2001). From breed variety, the fish such as turbot, lefteye flounder, river Puffer are vulnerable to the infection of the fine worm of shield most, and the example of being annihilated after infecting happens occasionally.

The fine Eimeria facultative parasite of shield is sought free living generally speaking, take the fine particulate matter (bacterium, little algae, protozoon etc.) that suspends for eating. But under some environment, these infusorians can show as parasitics, become saprophagous animal, engulf the cell of some mollusk, shell-fish, fish and organize residue and in its tissue the growth, the breeding (Yoshinaga T et al.1993), thereby cause tissue injury.

The research work of domestic and international generation for the sea fish paralembus digitiformis disease, aetology, taxology etc. only has the minority report, research to its control medicine then rarely has report, and be limited to the laboratory screening stage, have no for the production of compound prescription and patented product, and the oral drugs of Paralembus digitiformis disease are not reported both at home and abroad. At China, the traditional method of control Paralembus digitiformis disease is frequently to use highly concentrated formaldehyde solution, usually with 200～600 * 10^-6Concentration is soaked the fish body. Use formalin can only kill the shield infusorian of ill fish body surface, then without effect, and high concentration throws in and can cause Fish tissue to damage to the polypide in the intrusive body, and food fish residues of formaldehyde and serious environmental pollution.

Summary of the invention

The purpose of this invention is to provide a kind of aquatic products special-purpose medicaments that can treat the sea fish paralembus digitiformis disease and this disease be had certain preventive effect. By using this medicine to reach obvious result for the treatment of to the cultured fishes of suffering from Paralembus digitiformis disease, significantly improve the cultivation survival rate, cultured output and economic benefit.

The present invention makes by following technical scheme:

Its experiment flow is: carry out widely Paralembus digitiformis disease epidemiology survey, grasp its epidemic; Collect disease fish sample and carry out the separating of the fine worm of cause of disease shield, cultivation, evaluation and artificial resumption infection and test to determine to cause a disease former; Carrying out external medicine kills except testing with the screening medicine; Carrying out pharmaceutical formulation combination and clinical prevention effect test determines and optimization of C/C composites.

Its drug ingedient and proportioning are: Verbena officinalis 40%, hardship remonstrate with 30%, the root of large-flowered skullcap 30%, pulverize and sieve through 100 orders, mix to form.

The present invention and prior art contrast its characteristics:

1, the invention belongs to the compound medicine of Chinese herbal medicine of aquatic products special use, be applicable to oral form dispenser, the property of medicine is gentle and have no side effect, and is that the technology of orally taken for curing seawater fish shield balantidiasis is pioneering. Only need in proportion medicine to be added to during use to make pharmaceutical chemistry in the bait, method of operating is easy, with low cost, is applicable to large-scale seedling fostering and breeding production.

2, the drug ingedient of this medicine by the fish bulk absorption after, can significantly improve seawater fish to the resistance in the shield fibre. For the sick fish that is subjected to the fine insect infection of shield, can efficiently kill the infusorian in the fish body, overcome the defective that general pesticide is only applicable to the body surface desinsection. When the clinical testing proof was used for oral medication, the cure rate of this medicine can reach more than 80%.

3, Chinese herbal medicine is the green of generally acknowledging, the medicine that has no side effect. Use the present invention to reducing or replace the use of formaldehyde, to improve cultivation marketable fish quality and to ensure food safety and to play positive impetus.

The specific embodiment

The fine worm of shield can cause cultivating fish body systemic infection, and except body surface infected, ill fish interior tissue and organ found that also a large amount of polypides are parasitic. Therefore, the oral administration treatment obviously is absolutely necessary.

The inventor has taken the lead in carrying out the research of control Paralembus digitiformis disease special-purpose medicaments, has developed a kind of efficient, nontoxic special-purpose oral compound Chinese herb, called after " the fine worm of shield is clear ", and its application process carried out the clinical prevention demonstration. The result of clinical trial shows that these special-purpose oral drugs of employing can reach obvious prevention and curative effect to this disease.

During year May in January, 2004 to 2005, this research has been carried out clinical testing in man of the ground such as Yantai, Shandong, Qingdao, Weifang, sunshine, Wendeng City morbidities plant, the fish kind of test comprise turbot, lefteye flounder, river pure,

Fish etc. Application process and the actual therapeutic effect of this medicine determined in clinical trial, and table 1 has been summed up the operating position of each family.

Table 1 compound Chinese herb " the fine worm of shield is clear " is to the clinical prevention effect analysis of sea fish paralembus digitiformis disease

Time	The place	The field experiment man	Fish is used in test	Application process	Result of use
Time	The place	The field experiment man	Fish is used in test	Application process	Result of use	2004.1	Yantai	Haiyang	Young turbot	" the fine worm of shield is clear " is oral: the 5g/kg feed, every day twice, threw something and fed continuously 7 days; Dipping concentration is 10 * 10^-6, 12h/ times/day, continuous three times.	The state of an illness is effectively controlled, and curative effect is good. Infusorian is completely without vigor in the microscopy gill filament and the ascites, and quantity obviously reduces.
2004.2	Weifang	Changyi	The young turbot turbot	" the fine worm of shield is clear " oral lO days: 5g/kg feed; Use simultaneously the sea fish paralembus digitiformis pesticide dipping three times. " the fine worm of shield is clear " oral 14 days, the 5g/kg feed;	The death rate obviously reduces, and the state of an illness is effectively controlled. Infusorian is completely without vigor in the microscopy gill filament and the ascites, and quantity obviously reduces. The state of an illness is controlled, curative effect	2004.1	Yantai	Haiyang	Young turbot
2004.2	Weifang	Changyi	The young turbot turbot			2004.9	Qingdao	The Jiangzhou	Form fish	Simultaneously dipping three times, concentration 10 * 10^-6, 7h/ times/day.	Good. Infusorian is completely without vigor in the microscopy gill filament and the ascites, and quantity obviously reduces.
2004.9	Qingdao	Huang Island	Lefteye flounder is formed fish	" the fine worm of shield is clear " oral 10 days: 5g/kg feed; Use simultaneously the sea fish paralembus digitiformis pesticide dipping three times.	Infusorian disappears substantially in the microscopy gill filament and the ascites, and the state of an illness is effectively controlled.	2004.9	Qingdao	The Jiangzhou	Form fish
2004.9	Qingdao	Huang Island	Lefteye flounder is formed fish			2004.11	Wendeng City	Little sight	The river Puffer fish that survives the winter	Prophylactic: oral 7 days of every first quarter moon " the fine worm of shield is clear ", 5g/kg feed.	There is not balantidiasis to occur. Former years are dead more than half or be annihilated.
2005.1	Weifang	Han Ting	The turbot fry	" the fine worm of shield is clear " oral 12 days: 8g/kg feed; Dipping three days, concentration are 10 * 10^-6, 11h/ times/day.	Fall ill late period, survival rate is low. But the vigor of survival fish strengthens, and progressively cures. Infusorian is completely without vigor in the gill filament and the ascites.	2004.11	Wendeng City	Little sight	The river Puffer fish that survives the winter
2005.1	Weifang	Han Ting	The turbot fry			2005.5	Yantai	Haiyang Xin Cun	Turbot	" the fine worm of shield is clear " oral 7 days, the 5g/kg feed;	The state of an illness is controlled after three days, and fish lives

Form fish

Simultaneously dipping three days, concentration is 10 * 10^-6, 7h/ times/day.

Power is recovered, and obtains curing in seven days. Infusorian is completely without vigor in the gill filament and the ascites, and quantity obviously reduces.

Annotate: owing to the commercial interest relation, the title inconvenience of test site man is open in this table.

Drug dosage and using method:

(1) oral

Oral consumption: addition is 3～4 grams/kilogram feed during prevention, 5～6 grams during treatment/kilogram feed.

Orally administered method: according to above-mentioned drug dosage and the bait weight of throwing something and feeding, take by weighing in proportion medicine. First medicine is mixed with egg (1～2 egg/per kilogram bait) and beat evenly with mixer, its mixture is slowly poured in the dryness granular bait for fish, one side predominate mixing makes medicine evenly be adhered to the granular bait for fish surface; Spread out and can throw something and feed after the lucifuge airing solidified egg in 2 hours. Then directly in proportion medicine is incorporated in the bait raw material for moist bait, stirs, extrusion modling is made pharmaceutical chemistry and is thrown something and fed after freezing. Sooner or later throw something and feed every day two meal pharmaceutical chemistry, throwing something and feeding continuously 10～14 days is a course for the treatment of.

(2) dipping

The dipping consumption: concentration is 10 * 10 during dipping^-6, namely every cube of water body adds 10 gram medicines.

Dipping application process: by the appeal consumption, take by weighing first an amount of medicine and be soaked in the hot water, boil elimination residue after 3～4 hours with iron or earthen container temperature, get and splash in the fishpond after leachate suitably dilutes. Dipping every day required to be used in conjunction 3 days about 10 hours. During the dipping treatment, stop exchanged water, but will keep suitable water level and sufficient dissolved oxygen.

Prove that by a large amount of control clinical testings the treatment of this special medicament has obvious result for the treatment of to Paralembus digitiformis disease. For many cultivation producer has retrieved a large amount of economic losses, the curative effect that tested raiser generally reacts " shield fibre worm is clear " is good in the clinical practice process. The present invention has started the precedent of sea fish paralembus digitiformis disease herbal control technology, to improving the cultivation survival rate, ensures that breeding production stable and high yields tool is of great significance.

Claims

1, a kind of compound compound method of Chinese herbal medicine of preventing and treating seawater fish shield balantidiasis is characterized in that its drug ingedient and proportioning, and its weight ratio is: Verbena officinalis 40%, hardship remonstrate with 30%, the root of large-flowered skullcap 30%, pulverize and sieve through 100 orders, mix to form.