CN1699398A - 一种联合分离纯化熊果酸和寡聚原花色素的工艺及用途 - Google Patents
一种联合分离纯化熊果酸和寡聚原花色素的工艺及用途 Download PDFInfo
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Abstract
本发明属于生化分离纯化技术领域，本发明所述从山楂中分离、纯化寡聚原花色素和熊果酸生产工艺，从新鲜山楂或干果中用乙纯抽提，抽提物去乙醇后，冷藏。取出离心分离，上清液上聚酰胺柱，洗涤、洗脱、喷雾干燥，得原花色素干粉。沉淀物水洗，烘干，得熊果酸产品。本品可制成片剂、胶囊、咀嚼片、口服液系列制剂，为抗炎、抗菌、抗糖尿病、抗溃疡、降血脂等的保健食品。进一步纯化可制成药品。
Description
一.技术领域
本发明属于生化分离技术领域。
二.背景技术
山楂寡聚原花色素技术见发明专利[11]公开号CN1357382A，熊果酸的提取工艺已有专利申请，申请号为200310111479.8，其方法是从女贞叶中提取熊果酸。但从山楂中直接提取熊果酸的方法未见报导或专利，而用山楂为原料联合提取熊果酸和寡聚原花色素两种物质的方法也未见报导。熊果酸(Ursolic acid)又名乌索酸、乌苏酸，属三萜类化合物，它在自然界分布很广，如杜鹃花科植物熊果的叶、果实；玄参科毛泡桐的叶，以及木樨科植物女贞的叶、蔷薇科的山楂的果实中均已发现，含大量熊果酸。熊果酸具有镇静、抗炎、抗菌、抗糖尿病、抗溃疡、降低血脂的作用。据《中国药物杂志》1997年6卷2期报导，它不仅对多种致癌、促癌物有抵抗作用，而且对多种恶性肿瘤细胞例如P388和L1210白血病细胞、A549人肺腺细胞有抑制生长作用，能诱导F9畸胎瘤细胞分化和抗血管生成作用。
三.发明内容
本发明需要解决的问题是从山楂中联合提取寡聚原花色素和熊果酸两种物质，并利用两者关系互补作用制成片剂、胶囊、咀嚼片、口服液等。作为抗氧化、降血脂、降血糖、抗溃疡、抗菌、抗炎的保健食品，进一步纯化可制成药品。
本发明技术方案：
1、熊果酸和寡聚原花色素提取工艺：山楂洗净，加入95％乙醇1-2倍体积，捣碎，间歇搅拌浸取12-24小时，过滤、滤渣再用95％乙醇提取一次，过滤，合并滤液，滤液蒸馏去乙醇后，冷藏12-24小时，解冻后离心，上清液含原花色素，用聚酰胺柱吸附、水洗去杂质，60％乙醇洗脱，洗脱液蒸去乙醇，喷雾干燥，得寡聚原花色素干粉，纯度达80％以上。离心沉淀物，用蒸馏水洗2-3次，取出60℃烘干得熊果酸干粉，纯度达80％以上。。
2、原花色素的测定-盐酸-正丁醇法。
本方法利用原花色素在盐酸-正丁醇溶液中加热生成花色素，其在525nm产生特征性光吸收，以光吸收值大小参比标准曲线来确定原花色素的含量[Butler L.G：J.Agric Food Chem.1982，30，1090-1094。配制含有30％盐酸-正丁醇50ml，备用。分别取参比标准液1mg/ml 25μl、50μl、75μl、100μl，4mg/ml 50μl，加1.4ml盐酸-正丁醇溶液，加水使总体积到1.5ml，以锡箔封试管口，100℃加热30分钟，冷却后，在波长为525nm比色，绘制标准曲线。OPC样品配成1mg/ml，取0.1ml按同法测定525nm光吸收，对照标准曲线分别计算各组的原花色素含量。
3、熊果酸测定-高氯酸-香草醛法。
依据熊果酸在高氯酸作用下能与香草醛产生颜色反应，在546nm处有最大吸收。山楂熊果酸含量可通过参比熊果酸标准品标准曲线，计算含量和纯度。
熊果酸标准品，精确配制1mg/ml，取0.05、0.1、0.2、0.3、0.4、0.5ml，分别置5ml容量瓶，100℃烘干，冷却后，每瓶加0.5ml 5％香草醛0.8ml高氯酸，摇匀置60℃水浴保温10分钟，取出后，用乙酸定容至5ml，摇匀后，546nm测定光吸收值，以浓度对光吸收值作标准曲线。山楂熊果酸样品同法配制1mg/ml样品液，取0.1、0.2、0.3ml分别置5ml容量瓶100℃烘干后，加0.5ml 5％香草醛0.8ml高氯酸60℃水浴保温10分钟后，加乙酸至刻度，546nm测定光吸收值。对比标准曲线计算样品每毫克的熊果酸含量。
本发明与现有技术相比，其有益效果是在一种原料山楂中，可以一次提取两种有效产物，提高了原料的利用率，降低了产品的成本，而且两种产物在抗肿瘤药和保健品应用方面都有良好的前景，可制成片剂、胶囊、咀嚼片、口服液系列制剂，在制备降血脂保健食品中应用。
四.具体实施方案
取新鲜山楂2000克，加入95％乙醇匀浆，共加乙醇2400ml，浸泡12小时，过滤，得滤液2760ml，渣子再加入2400ml 95％乙醇放置12小时，过滤，得滤液2500ml，合并滤液得5260ml，减压蒸去乙醇，得溶液1000ml，放置冷藏柜12小时。取出离心，得上清液。
1、上清液加入1倍量蒸馏水，上聚酰胺柱吸附，蒸馏水洗去杂质，60％乙醇洗脱，得乙醇洗脱液，减压蒸去乙醇，得200ml悬浊液，冻干，得原花色素9.96克，含量89％。
2、离心沉淀物，先用蒸馏水洗沉淀物2-3次，得浅绿色沉淀，60℃烘干，得熊果酸粗品5.36克，含量85％。将粗制的熊果酸加蒸馏水，调pH11，溶解离心去除沉淀，上清液调pH3，离心得沉淀，干燥后可得95％以上的精制熊果酸。
含量和纯度检查：(1)原花色素用盐酸-正丁醇法。
本方法利用原花色素在盐酸-正丁醇溶液中加热生成花色素，其在525nm产生特征性光吸收，以光吸收值大小参比标准曲线来确定原花色素的含量[Butler L.G：J.Agric Food Chem.1982，30，1090-1094。配制含有30％盐酸-正丁醇50ml，备用。分别取参比标准液1mg/ml 25μl、50μl、75μl、100μl，4mg/ml 50μl，加1.4ml盐酸-正丁醇溶液，加水使总体积到1.5ml，以锡箔封试管口，100℃加热30分钟，冷却后，在波长为525nm比色，绘制标准曲线。OPC样品配成1mg/ml，取0.1ml按同法测定525nm光吸收，对照标准曲线分别计算各组的原花色素含量。
(2)熊果酸测定用高氯酸-香草醛法。
依据熊果酸在高氯酸作用下能与香草醛产生颜色反应，在546nm处有最大吸收。山楂熊果酸含量可通过参比熊果酸标准品标准曲线，计算出含量和纯度。
熊果酸标准品，精确配制1mg/ml，取0.05、0.1、0.2、0.3、0.4、0.5ml，分别置5ml容量瓶，100℃烘干，冷却后，每瓶加0.5ml 5％香草醛0.8ml高氯酸，摇匀置60℃水浴保温10分钟，取出后，用乙酸定容至5ml，摇匀后，546nm测定光吸收值，以浓度对光吸收值作标准曲线。山楂熊果酸样品同法配制1mg/ml样品液，取0.1、0.2、0.3ml分别置5ml容量瓶100℃烘干后，加0.5ml 5％香草醛0.8ml高氯酸60℃水浴保温10分钟后，加乙酸至刻度，546nm测定光吸收值。对比标准曲线计算样品每毫克的熊果酸含量。
Claims (5)
1.一种从山楂中联合分离纯化熊果酸和寡聚原花色素的生产工艺，其特征是新鲜山楂或干山楂片，洗净、捣碎后加入食品级95％乙醇2-3倍，浸提，12-24小时，间歇搅拌过滤，滤液放置备用，渣子用同样方式再提取一次，两次滤液合并，减压蒸馏去乙醇后冷藏24小时，次日离心，上清液留做寡聚原花色素，沉淀物制备熊果酸。
2.根据权利要求1所述生产工艺，其特征是生成的含寡聚原花色素上清液加入0.5-1倍蒸馏水，用聚酰胺柱吸附，先用水洗杂质，再用60％乙醇洗脱，洗脱液去乙醇后，喷雾干燥，可得棕红色的粉末，即为寡聚原花色素，纯度达90％。
3.根据权利要求1所述生产工艺，其特征是得到的抽提沉淀物，用蒸馏水洗3-5次，离心、干燥，可得到纯度约80％的熊果酸粗制品。将粗制的熊果酸加蒸馏水，调pH11，溶解离心去除沉淀，上清液调pH3，离心得沉淀，干燥后可得95％以上的精制熊果酸。
4.根据权利要求1生产工艺生产的熊果酸、寡聚原花色素，其特征是可制成片剂、胶囊、咀嚼片、口服液系列制剂，在制备降血脂保健食品中应用。
5.根据权利要求1生产工艺生产的熊果酸、寡聚原花色素在制备治疗肿瘤药物中的应用。
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| CN101507727B (zh) * | 2009-03-26 | 2010-09-29 | 上海交通大学 | 降血脂的中药组合物及其制备方法 |
| CN101983965A (zh) * | 2010-10-26 | 2011-03-09 | 武汉利元亨药物技术有限公司 | 从山楂中精提熊果酸的方法 |
| CN102229631A (zh) * | 2011-05-03 | 2011-11-02 | 西安瑞联近代电子材料有限责任公司 | 一种从葡萄皮红中分离纯化锦葵花素葡萄糖苷的方法 |
| WO2012024399A3 (en) * | 2010-08-19 | 2013-06-27 | Johnson & Johnson Consumer Companies, Inc. | Compositions comprising paulownin and/or paulownia extracts and uses thereof |
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| CN1126757C (zh) * | 2001-12-20 | 2003-11-05 | 武汉大学 | 从女贞叶中提取熊果酸的方法 |
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