CN1696280A - Technique for planting north aweto in rice bag in high efficiency - Google Patents

Abstract

A technology for culturing the efficient northern cordyceps in bagged rice includes dissolving the special additive in water, adding the solution to the rich grains in plastic bag, tying the mouth of plastic bag, sterilizing at 100 deg.C for 1 hr, cooling, inoculating the bacterial seeds, and culturing. Its rate of finished product is more than 99%.

Description

The rice bag is planted high benefit Cordyceps militaris (L.) Link. technology

Cordyceps militaris (L.) Link. claims Cordyceps militaris, Cordyceps militaris (L.) Link. again, is a kind of famous and precious medicinal fungi with higher value medical health care and economic worth.According to modern medicine research and clinical efficacy checking: Cordyceps militaris (L.) Link. (hereinafter to be referred as Cordyceps militaris) has multiple medical care effects such as antifatigue, hypoxia tolerance, anti-ageing, anticancer and raising immune function of human body.Contain multiple amino acids, the VITAMIN of cordycepin, Cordyceps polysaccharide, protein, Nucleotide, superoxide-dismutase (SOD), needed by human according to surveying and determination in the Cordyceps militaris fruiting body and contain 20 various trace elements such as copper, zinc, selenium, and multiple nutrients active substance and anti-ageing isoreactivity material, with the drug effect basically identical of natural cordyceps.In recent years, because the raising of people's living standard, the consumption of natural cordyceps with medicinal health care function is constantly increased, limited resources is exhausted day by day.Along with tame continuous development of Cordyceps militaris and Application and Development, the human consumer begins to turn to artificial cordyceps, and price constantly rises suddenly and sharply and the deficiency of the natural cordyceps that resource reduces day by day to substitute.

At present, the Cordyceps militaris artificial culture, mainly be to be raw material with the rice, add a small amount of peptone, glucose, VITAMIN and dried silkworm chrysalis meal etc. with Cans cultivations (have raw material is wide, the cycle is short, labour intensity low), as grasp technology, relatively benefit is higher than conventional edible medicinal fungus cultivation (at China's coastal flourishing city per kilogram quality about 1500 yuan of artificial cordyceps preferably, about 600～800 yuan of inland city per kilogram, as be processed into medicine and the healthcare products valency is higher).So many people invest cultivation one after another, because of the condition of growing of Cordyceps militaris is understood very less, condition is simple and crude, Technology is complicated, lack multiple reasons such as recognition capability, the substratum nutrition-allocated proportion of production technology and experience, no bacterial classification seed selection and quality good or not be improper, seriously polluted, poor growth often takes place in cultivation, annesl does not go out grass (sporophore), bottle plants that substratum nutrition is few, production cost is high in addition, output and benefit be lower, and what have ends in failure.In order to find a kind of science practical simplicity, to be difficult for polluting, effectively improving the new technology of cultivating success ratio, output and benefit, be agricultural-food such as rice cultivation high benefit Cordyceps militaris, and edible medicinal fungus is realized increase wealth, improve agricultural performance, promote that traditional agriculture develops to efficient ecological and sustainable agriculture direction.Applicant is through repetition test for many years, filtered out the Cordyceps militaris excellent species finally and with the simple and practical new technology of rice cultivation high benefit Cordyceps militaris.

Plant the high benefit Cordyceps militaris with the rice bag, do not consume forest tree resource, do not take grainfield, with the rice is main raw material, adding the antifouling growth encourage of a small amount of collection nutrition is many effects Chinese caterpillar fungus specialist additive (hereinafter to be referred as the Chinese caterpillar fungus specialist additive) of one, plastics bag, bacterial classification etc., cultivation Cordyceps militaris are that Higher output is not accompanied by a higher income has found a new way for agricultural-food such as rice.Improve the nutrition and the quality of rice with edible medicinal fungus such as Cordyceps militaris, produce edible medicinal fungus and sporophore product etc. and realize appreciating, compare, have characteristics such as time-saving energy-saving, less investment, cycle weak point, instant effect, high efficiency, easy application with traditional agriculture.

China is large agricultural country, rice-producing area is distributed more widely, except that minority agricultural condition is produced the grain quality rice in the area preferably, most areas are relatively poor because of agriculture condition, or multiple unfavorable factor influence, main production matter time higher conventional rice or the hybridisation rice of output, its price is starkly lower than grain quality rice, Higher output is not accompanied by a higher income, had a strong impact on agriculture benefit and peasant's kind grain enthusiasm.As reaching the working condition of grain quality rice; need to strengthen investment; increase facility and improve working condition; because agricultural production cycle is long; benefit is lower relatively; this mode does not meet the present situation of agricultural development of China; as with the higher processing of being rich in edible medicinal fungus such as multiple Cordyceps militaris or mycelium and goods of biological new technology rice (rice) cultivation benefit that matter time output high price is low with nutritional health function; improve its nutritional quality; be that (with rice is main raw material to a more simple and practical mode that increases income; add on a small quantity essential aid nutrition; can cultivate edible medicinal fungus or cultivate mycelium etc. by the mode of production of edible mushrooms); all be higher than traditional agriculture from aspects such as production cycle and benefits; but and mass-producing; anniversaryization; batch production is produced and is appreciated comparatively fast; as use the rice Xinbao mushroom culturing; Cordyceps militaris; glossy ganodermas etc. all can normal fruiting, and the substratum behind the fruiting can extract biochemical drug or make multiple use such as feed.Plant Cordyceps militaris or processing nutritive health-care rice and goods with the rice bag, can solve the bad disease that nutritive deficiency causes, improve physique and health.The Cordyceps militaris fruiting body that artificial culture goes out can be sold, edible, drinks when tea or healthcare products, also can be processed into healthcare products or medicine etc.Can improve traditional agriculture with present technique and produce infiltration and development, progressively add industrial or agricultural and sustainable efficient direction develops, improve constantly economic benefit, for people provide abundant healthy many kinds food to efficient ecological agricultural, sight-seeing agriculture, modern times.

The rice bag is planted high benefit Cordyceps militaris (L.) Link. technology, be based on the traditional cultivation method, adopt biochemical anti-soil technology and few products (specific anti-fouling agent for edible fungus) in the patent 001328875, combine with traditional grog sterilization anti-soil technology, not changing and influence under the condition that Cordyceps militaris (L.) Link. grows, substitute the novel method of Cans cultivation with plastics bag.During cultivation, the Chinese caterpillar fungus specialist additive is water-soluble in proportion in right amount, add in the rice be contained in the plastics bag behind the tying, sterilized 1 hour down at 100 ℃, taking out cooling back access bacterial classification in the disinfectant transfer room can cultivate, and does not need to add other supplementary material again, and assorted bacterium is difficult for polluting, the technology yield rate of grasping can reach more than 99%, and the grower of suitable different technologies level and condition uses.As a small amount of cultivation tens to the hundreds of bag, only need family expenses aluminum pot or food steamer continuous sterilization after, gradation or concentrate the inoculation back just can cultivate.Big as the amount of cultivation, after sterilizations such as normal-pressure sterilization pot, indoor inoculation, cultivation that just can be after sterilization.Present technique is compared with traditional method, has less investment, raw material is wide, technology is simple and practical, time-saving energy-saving, be difficult for characteristics such as pollutions, yield increase effect height, easy application.

The rice bag is planted high benefit Cordyceps militaris (L.) Link. technology, be with the Chinese caterpillar fungus specialist additive water-soluble in proportion after, add in the rice be contained in the plastics bag behind the tying, sterilized 1 hour down at 100 ℃, taking out cooling access bacterial classification in the disinfectant transfer room can cultivate, do not need to add other supplementary material, assorted bacterium is difficult for polluting again, and the technology yield rate of grasping can reach more than 99%.Simple and practical, characteristics such as cultivation benefit is high, easy application possess skills, the grower of different technologies level such as suitable Chinese caterpillar fungus cultivation factory, Medicines and Health Product factory, Development of Eco-tourism, edible mushrooms factory, food processing plant, food and drink, specialist and the individual that there is lack of raw materials and benefit is lower and condition uses, solved with traditional complicated technology and cultivated difficult problems such as easy pollution, output and benefit are lower, be agricultural-food such as rice cultivation high benefit edible medicinal fungus, increase the benefit with the processing appreciation, a new way that technology is simple and practical is provided.

The present technique main raw material is a rice, and auxiliary material is the Chinese caterpillar fungus specialist additive, and all the other are water, plastics bag, Cordyceps militaris bacterial classification etc.Rice should be chosen food grade does not have old rice, hybridization rice, matter time rice or the polished rice that the unharmful substance that goes mouldy pollutes, yet available broken corn, millet etc.Water: should select the well water or the tap water that meet food sanitation standard for use.Plastics bag: should select high-quality edible mushroom polypropylene knuckle bag for use.The Cordyceps militaris bacterial classification: should select that adaptability is strong for use, the pure white growth of mycelia is fast, can well grow on the substratum of Chinese caterpillar fungus specialist additive preparation, mycelia send out substratum full after after stimulating with illumination under the thermophilic comparatively fast annesl go out grass, have speed and give birth to the bacterial classification of good quality and high output characteristics and be used for cultivating.Have this characteristics excellent No. 2 as north, suitable Chinese caterpillar fungus grower and annually cultivating are used.Also can introduce test mass and be applied to cultivation after reliable.Chinese caterpillar fungus specialist additive: form by the essential peptone of Cordyceps militaris, glucose, VITAMIN and a small amount of antifouling raw material scientific formulas such as additive, collection nutrition is antifouling, the compound specialist additive of growth encourage effect, can effectively solve improper because of nutrition-allocated proportion, produce the poor growth that causes such as more extensive, easy problem such as pollutions, excellent No. 2 bacterial classifications of this agent and north by Ningqiang County, Shaanxi two youth dam edible mushrooms development departments develop, seed selection provides.The oxygenation agent: can be from relevant chemical industry shop or the chemical plant buy.

The rice bag is planted the method for high benefit Cordyceps militaris (L.) Link.:

1, culture medium prescription:

1. production of hybrid seeds culture medium prescription (suitable system original seed): rice 1000 grams, special-purpose stain control agent 36 grams of the Chinese caterpillar fungus production of hybrid seeds, water 1500～2000 grams (should according to the water-absorbent of different rice suitably increase and decrease to suitable, down with).

2. culture medium for cultivating prescription: 100 kilograms in rice, 3.0 kilograms of Chinese caterpillar fungus cultivation specialist additives, oxygenation agent 0.2～0.4% is about water 1500～2000 grams.

2, compound method: in the culture medium prescription ratio, elder generation's weighing rice, every bag of optional 40 grams, the amounts of 50 grams or 100 grams take by weighing branch and pack in the plastics bag, 50 ℃ of left and right sides hot water of weighing are poured in clean the porcelain basin or plastic tub then, at last the Chinese caterpillar fungus specialist additive is put into hot water, stirring dissolving back fully (nutritive medium) repeatedly just can take by weighing nutritive medium by material-water ratio (being the amount ratio of rice and nutritive medium) to pour in the rice in the plastics bag (be quickening preparation speed, can make a measuring device of measuring rice and nutritive medium respectively with Plastic Bottle in advance), the intact back of packing will each plastics bag middle part with the plastics jag and oral area each prick, can sterilize after according to said method having pricked the cultivation pocket.

3, sterilization: after in Autoclave, adding suitable quantity of water earlier, put on the steaming frame of Autoclave cultivating pocket, close the lid and obturage, begin to heat up, kept about 1 hour when temperature reaches 100 ℃ (promptly emitting steam), substratum is cooked into the rice rice dumpling, put into transfer room after the taking-up cooling, with high-quality aerial fog disinfectant or the airtight sterilization of common method 30 minutes, the clothes after the promptly wearable sterilization entered the inoculation indoor inoculation.

4, inoculation: enter transfer room and should close door (preventing to contain the air admission of assorted bacterium) rapidly, with inoculating tool (inoculation is colluded, tweezers, inoculation spoon etc.), bacterial classification outer wall (female kind, original seed), operator's both hands etc. cause that easily the article that bacterial classification and culture material pollute, the alcohol with 75% carry out beginning inoculation after wiping or the dipping sterilization.Now mainly introduce the method that (easy application) inoculated with solid rice bacterial classification.1. change to expand cultivation with the test tube original seed and use original seed: take out earlier behind the test tube plug to collude and take beans size bacterial classification 2-3 piece, or the access of grain of rice size bacterial classification several piece separates on the cultivation basal plane of cultivating the pocket jag, tighten sack slightly and get final product with the disinfectant inoculation.When 2. changeing the expansion cultivating bag with original seed, with disinfectant tweezers or inoculation spoon bacterial classification is smash into finger or beans size bulk earlier, with the inoculation spoon bacterial classification is inserted to separate on the cultivation basal plane of cultivating the pocket jag then and (during inoculation sack is drawn back 2-3 centimetre of big small pore-like), every bag can be inserted 5-10 gram bacterial classification, be evenly distributed on the charge level as far as possible.Can adapt to the increase inoculum size as conditions permit, grow quickly, connect and tightened sack slightly by former tying method behind the bacterial classification and get final product in order to mycelia.After having connect original seed and cultivating bag by above-mentioned inoculation method, can move in the culturing room of sterilization and moth proofing, be placed on the culturing rack and cultivate.

5, cultivate: low temperature mushroom during Cordyceps militaris belongs to, the mycelial growth temperature scope is 5-30 ℃, and the mycelia optimum growth temperature is 15-25 ℃, and the stroma optimum growth temperature is 16-22 ℃, all slow down if be below or above the optimum temperuture growth, being higher than can very fast death more than 30 ℃.When therefore cultivating, should be according to its this characteristic, at first culture temperature is remained on and carry out the shading cultivation under 15-18 ℃, when mycelial growth to substratum half or about 2/3rds, temperature can be risen to about 20 ℃, as cultivating pocket tying degree of tightness appropriateness, temperature is suitable, left and right sides mycelia can send out purseful in 20 days, as did bacterial classification and can use or preserve.

As cultivate, after the mycelia purseful, can suitably cultivate again several days according to growing state, make mycelia accelerate decomposition substratum accumulation nutrition, when the hyphal peg time-out appears in substratum top, begin to give the thermal stimulation about 10-15 ℃, and increase light stimulation annesl, can use natural light daytime, and can shine evening with fluorescent lamp, promotes very fast and even annesl.Suitable as temperature, bacterial classification is good, left and right sides mycelia can change look in 7-10 days, the sterilization of reply culturing room is 30 minutes behind the annesl, separate then and cultivate pocket middle part jag, both hands draw the bag middle part flat, are converted into straight-tube shape with the handgrip sack to the bag indent, for the stroma growth provides the space.When stroma grows to about 1 centimetre, should be in time to the cultivating bag oxygenation of ventilating, method is evenly to sting micropore with the disinfectant pin around sack, or separate the sack jag and add cover disinfectant non-cotton cover, or separate the sack jag and sack is drawn downwards put plastics bag, and temperature being remained under 18-22 ℃, it is (not enough as humidity that humidity remains on 80-90%, can or on indoor stove, put the adjustings such as aluminum pot of adorning water in culturing room's ground watering), and according to growth ventilation in good time.In cultivation, must guard against bigger thermal stimulation, should keep illumination uniformly, can not make temperature too high too big with humidity, should with temperature and humidity regulation and control in the suitableeest scope in order to its normal growth, avoid lopsided sporophore to take place, when stroma is the bar-shaped not regrowth of orange or orange, can open sack adopts down from the stroma base portion with pocket knife, tweezers or scissors, try not to damage, put into clean utensil in time oven dry or airing after adopting down, under sunlight, do not shine in order to avoid sporophore is faded.Dried stroma should leave in the airtight plastics bag or sell.

After gathering, as substratum lack of water part, available cold water or nutritive medium, according to the situation that moisture content reduces, every bag adds about the 5-10 milliliter, keeps suitable temperature to allow it recover growth then, manages the 1-2 stubble seat of also can gathering again again by above method.

Claims (3)

1, the rice bag is planted high benefit Cordyceps militaris (L.) Link. technology, be based on the traditional cultivation method, adopt biochemical anti-soil technology and few products (specific anti-fouling agent for edible fungus) in the patent 001328875, combine with traditional grog sterilization anti-soil technology, not changing and influence under the condition that Cordyceps militaris (L.) Link. grows, substitute the novel method of Cans cultivation with plastics bag.It is characterized in that: be raw material with rice, Chinese caterpillar fungus specialist additive, water, plastics bag etc., during cultivation, the Chinese caterpillar fungus specialist additive is water-soluble in proportion in right amount, add in the rice be contained in the plastics bag behind the tying, 100 ℃ of down sterilizations 1 hour, take out the cooling back and in the disinfectant transfer room, insert bacterial classification and can cultivate, do not need to add again other supplementary material, assorted bacterium is difficult for polluting, the technology yield rate of grasping can reach more than 99%, and the grower of suitable different technologies level and condition uses.As a small amount of cultivation tens to the hundreds of bag, only need family expenses aluminum pot or food steamer continuous sterilization after, gradation or concentrate the inoculation back just can cultivate.Big as the amount of cultivation, after sterilizations such as normal-pressure sterilization pot, indoor inoculation, cultivation that just can be after sterilization.Present technique is compared with traditional method, has less investment, raw material is wide, technology is simple and practical, time-saving energy-saving, be difficult for characteristics such as pollutions, yield increase effect height, easy application.

2, rice bag according to claim 1 is planted high benefit Cordyceps militaris (L.) Link. technology, it is characterized in that: main raw material is rice or broken corn etc., and auxiliary material is the Chinese caterpillar fungus specialist additive, and all the other are plastics bag, water, bacterial classification etc.

3, rice bag according to claim 1 is planted high benefit Cordyceps militaris (L.) Link. technology, it is characterized in that: according to its characteristic of growing, and to demands such as nutrition, culture medium prescription is made up of production of hybrid seeds substratum and culture medium for cultivating:

1. production of hybrid seeds culture medium prescription (suitable system original seed): rice 1000 grams, Chinese caterpillar fungus production of hybrid seeds specialist additive 36 grams, water 1500～2000 grams (should according to the water-absorbent of different rice suitably increase and decrease to suitable, down with).

2. culture medium for cultivating prescription: 100 kilograms in rice, 3.0 kilograms of Chinese caterpillar fungus cultivation specialist additives, oxygenation agent 0.2～0.4% is about water 1500～2000 grams.