CN102860497B - Functional Pleurotus eryngii food and preparation method thereof - Google Patents
Functional Pleurotus eryngii food and preparation method thereof Download PDFInfo
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Abstract
The invention discloses functional Pleurotus eryngii food and a preparation method thereof. The preparation method of the functional Pleurotus eryngii food includes the steps of subjecting the Pleurotus eryngii to solid fermentation to obtain Pleurotus eryngii food. Medium used in solid fermentation is composed of 80g of base material and 80-120ml (specifically 100-120ml, 100-110ml or 110-120ml) of water. The base material comprises at least one of polished round-grained rice, glutinous rice, polished long-grained non-glutinous rice and Job's tears. The functional Pleurotus eryngii food contains active nutrients such as proteins, polysaccharides, reducing sugars, amino acids and polyphenols. Ethanol extract and water extract of the functional Pleurotus eryngii food are high in antioxidant activity. The functional Pleurotus eryngii food is convenient and fast to eat and meets requirements of people for daily nutrition.
Description
Technical field
The present invention relates to biological technical field, be specifically related to a kind of pleurotus eryngii functional food and preparation method thereof.
Background technology
Pleurotus eryngii (Pleurotus eryngii) has another name called pleurotus eryngii, snow is fine and soft, belongs to Basidiomycetes, Agaricales, Tricholomataceae, Pleurotus.The fructification list life of pleurotus eryngii or all living creatures, during children, cap is less, and cap intermediate recess after ripe, color is ivory buff, bacterial context pure white.Pleurotus eryngii originates in the high mountain of the northern and Central Asia in south of europe, Africa, grassland, desert area, and also there is a small amount of distribution in China Qinghai, Sichuan, Xinjiang.After Henda in 1970 finds pleurotus eryngii on north India high mountain, within 1974, Cailleux has successfully cultivated strain of Pleurotus eryngii.The pleurotus eryngii majority that China cultivates at present introduces from Europe after the nineties in last century.
Pleurotus eryngii is nutritious, is rich in the mineral matter such as protein, carbohydrate, vitamin and calcium, magnesium, copper, zinc, can improves immune function of human body, has antiviral, anticancer, reducing blood lipid, the effect such as ease constipation stomach and beauty treatment.But pleurotus eryngii Time To Market is concentrated, and is not easy to deposit, and limits by eating method.
Summary of the invention
The object of this invention is to provide a kind of pleurotus eryngii functional food and preparation method thereof.
The invention provides a kind of method preparing pleurotus eryngii food, comprise the steps: to adopt solid fermentation culture medium that pleurotus eryngii is carried out solid fermentation, obtain pleurotus eryngii food; Described solid fermentation culture medium is made up of 80g base material and 80-120 milliliter (specifically can be 100-120 milliliter, as 100-110 milliliter or 110-120 milliliter) water; Described base material is at least one in polished rice, glutinous rice, long-grained nonglutinous rice and the seed of Job's tears.
The condition of culture of described solid fermentation can be 18-28 DEG C of (as 25 DEG C) lucifuge quiescent culture 20-60 days (specifically can be 30-50 days, as 30-35 days, 35-40 days or 40-50 days).
Also can comprise in described method and the product of described solid fermentation is carried out dry step.
Described drying specifically can be freeze drying and/or drying under reduced pressure.
Also can comprise in described method and dried product is carried out broken step.Product after described fragmentation can be the powdery of 80 mesh sieves.
The concrete steps of described solid fermentation are: the seed liquor of described pleurotus eryngii is seeded to described solid fermentation culture medium, then carries out described solid fermentation.In the step of described solid fermentation, specifically can by the seed liquor of pleurotus eryngii described in 5ml (as OD
600nmthe seed liquor of=0.75) be seeded to described solid fermentation culture medium.
The concrete preparation method of described seed liquor is as follows: by pleurotus eryngii mycelium inoculation to fluid nutrient medium, and 25-28 DEG C (as 25 DEG C) lucifuge shaken cultivation 7-10 days (as 7 days), obtain seed liquor.
The concrete formula of described fluid nutrient medium (natural pH) is: 4 grams of glucose, 10 grams of malt extracts (MaltExtract), 4 grams of yeast extracts (Yeast Extract) are water-soluble, is settled to 1 liter with water.
Arbitrary described pleurotus eryngii specifically can be the bacterial strain that CGMCC deposit number is 5.775 above.
The present invention also protects a kind of pleurotus eryngii food; its total amino acid content is 40%(mass ratio) more than, essential amino acids content is 10%(mass ratio) more than, content of reducing sugar is 3%(mass ratio) more than, Determination of Polyphenols is 0.2%(mass ratio) more than, total starches content is 1%(mass ratio) more than, protein content is 1%(mass ratio) more than, adenosine content is more than 0.1 ‰ (mass ratioes).
The total amino acid content of described pleurotus eryngii food specifically can be 40%-60%(mass ratio) (as 41%-50% or 50%-56%), essential amino acids content specifically can be 10%-20%(mass ratio) (as 12%-14% or 14%-16%), content of reducing sugar is 3%-5%(mass ratio) (as 3.6-4.0%, 4.0%-4.1% or 4.1%-4.2%), Determination of Polyphenols specifically can be 0.2%-0.5%(mass ratio) (as 0.3%-0.4% or 0.4%-0.5%), total starches content specifically can be 1.7%-2.5%(mass ratio) (as 1.8%-2.1%, 2.1%-2.3% or 2.3%-2.4%), protein content specifically can be 1.2%-14%(mass ratio) (as 1.3%-1.4% or 1.4%-13%), adenosine content specifically can be 0.14 ‰-0.18 ‰ (mass ratio) (as 0.14 ‰-0.17 ‰ or 0.17 ‰-0.18 ‰).
Described must amino acid be threonine, valine, methionine, isoleucine, leucine, phenylalanine, lysine and tryptophan.
Described pleurotus eryngii food specifically can be the pleurotus eryngii food that above arbitrary described method prepares.
Described pleurotus eryngii food can as the additive of other functional food, for the preparation of other food.Pleurotus eryngii functional food provided by the invention, in faint yellow, there is the fragrant and pleurotus eryngii fructification fragrance of light rice, its edible way is not limited only to ground rice, can also be made into the various dessert such as biscuit, cake, also can join in staple food as nutritional supplemental.
Pleurotus eryngii functional food provided by the invention contains protein, polysaccharide, reduced sugar, amino acid and polyphenol isoreactivity nutritional labeling, adenosine content is more than 0.1 ‰, and its ethanol extract and water extract all demonstrate very strong antioxidation activity (ferrous ion reducing power and sequestering power).
Pleurotus eryngii functional food provided by the invention, instant is quick, can meet people's daily nutrition demand.
Detailed description of the invention
Following embodiment is convenient to understand the present invention better, but does not limit the present invention.Experimental technique in following embodiment, if no special instructions, is conventional method.Test material used in following embodiment, if no special instructions, is and purchases available from routine biochemistry reagent shop.Quantitative test in following examples, all arranges and repeats experiment for three times, results averaged.
Pleurotus eryngii (pleurotus eryngii used in embodiment, Pleurotus eryngii) bacterial classification purchased from Institute of Microorganism, Academia Sinica's culture presevation administration committee common micro-organisms center (be called for short CGMCC, address: Datun Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica, postcode 100101), CGMCC is numbered 5.775.
The preparation of the functional ground rice of embodiment 1, pleurotus eryngii
1, preculture
Be seeded to by pleurotus eryngii quel strains in PDA medium slant, 25 DEG C of lucifuges are cultivated 7 days (mycelia covers with whole inclined-plane).
PDA culture medium (natural pH): take 200g potato and be cut into small pieces, the well-done rear filtered through gauze that adds water also collects filtrate, adds 20 grams of glucose and 16 grams of agar powders, be settled to 1 liter with water in filtrate.
2, seed liquor is prepared
Get mycelia from the inclined-plane of completing steps 1 and be forwarded to fluid nutrient medium, 25 DEG C, lucifuge, 180 revs/min of shaken cultivation 7 days, obtain seed liquor (O
d600nm=0.75).
Fluid nutrient medium (natural pH): by 4 grams of glucose, 10 grams of malt extracts (Malt Extract), 4 grams of yeast extract (Yeast Extract; Purchased from Oxoid Ltd., lot number is 1074139) water-soluble, be settled to 1 liter with water.
3, solid fermentation
Get the seed liquor that 5 milliliters of steps 2 obtain, be seeded to solid medium (being made up of 80g glutinous rice and 120 ml waters), 25 DEG C of lucifuge quiescent culture 40 days.
4, post processing
The whole cultivating system completing solid fermentation is carried out successively freeze drying and pulverizing (80 order), the freeze-dried powder obtained is the functional ground rice of pleurotus eryngii.
5, place after glutinous rice sterilizing 40 days, directly carry out freeze drying and pulverizing, obtain glutinous rice freeze-dried powder, as the contrast of the functional ground rice of pleurotus eryngii.
The Analysis of Nutritive Composition of the functional ground rice of embodiment 2, pleurotus eryngii
The functional ground rice of pleurotus eryngii obtain embodiment 1 and glutinous rice freeze-dried powder carry out Analysis of Nutritive Composition respectively.
Crude protein content adopts Kjeldahl nitrogen determination, and the protein conversion factor is 6.25.
Crude fat content adopts Soxhlet fat extraction process to measure.
Thick many candies (total starches) content sulfuric acid anthrone method measures.
Content of reducing sugar utilizes Forint phenol method to measure.
Determination of Polyphenols adopts determined by ultraviolet spectrophotometry (in gallic acid).
Amino acid content adopts acid hydrolysis PITC column front derivation rp-hplc determination; Agilent high performance liquid chromatograph device 1200, Venusil-AA amino acid analysis dedicated columns (4.6*250mm, 5 μm), column temperature is 40 degrees Celsius, and determined wavelength is 254nm; Mobile phase A: take 15.2g anhydrous sodium acetate, adds water 1850 milliliters, and after dissolving, glacial acetic acid adjustment pH is 6.5, and then add acetonitrile 140 milliliters, mixing is filtered stand-by; Mobile phase B: 80%(volumn concentration) acetonitrile solution; Flow velocity: 1 ml/min; (mobile phase is the mixed liquor of mobile phase A, Mobile phase B or mobile phase A and Mobile phase B to gradient, be linear gradient change): 0-min, 0% Mobile phase B, 2-15min, 0-10%B, 15-25min, 10-30%B, 25-33min, 30-45%B, 33-33.1min, 45-100%B, 33.1-38min, 100%B.
Analysis of Nutritive Composition the results are shown in Table 1 and table 2.
The main nutrient composition of the functional ground rice of table 1 pleurotus eryngii
| Glutinous rice freeze-dried powder | The functional ground rice of pleurotus eryngii | |
| Crude protein (mg/g) | 28.17±1.41 | 133.42±1.27** |
| Crude fat (mg/g) | 2.16±0.21 | 13.92±0.32** |
| Total starches (mg/g) | 11.46±0.02 | 22.46±0.03** |
| Reduced sugar (mg/g) | 15.67±1.33 | 40.67±1.25** |
| Total polyphenols (mg/g) | 0.55±0.00 | 4.84±0.01** |
The content of each free amino acid in the functional ground rice of table 2 pleurotus eryngii
As shown in table 1, compared with glutinous rice freeze-dried powder, the crude fat of the functional ground rice of pleurotus eryngii, crude protein, polysaccharide, reduced sugar, Determination of Polyphenols all significantly increase.As shown in table 2, compared with glutinous rice freeze-dried powder, the total amino acid content of the functional ground rice of pleurotus eryngii has brought up to 556.72mg/g from 259.09mg/g, essential amino acids content brings up to 139.37mg/g from 79.6mg/g, and in the functional ground rice of pleurotus eryngii, tyrosine is primary amino acid (its content is 213.17mg/g).Essential amino acid is the amino acid that human body can not oneself synthesize, and must pass through food supply, therefore the essential amino acids content of high concentration provides theoretical foundation for pleurotus eryngii tunning is developed to a kind of functional food.
The Determination of Adenosine of the functional ground rice of embodiment 3, pleurotus eryngii
The Main Function of adenosine makes cardiac arrhythmia patient recover sinus rhythm, reports that adenosine is relevant with the deep layer cerebral irritation of the ill people of other brains to alleviation Parkinson's in addition.
1, the making of calibration curve
Take adenosine standard items (Chinese pharmaceutical biological product inspection institute, article No. is 110879-200202) 2.0 milligrams, being placed in 100 milliliters of volumetric flasks, with containing 15%(volumn concentration) methanol aqueous solution of methyl alcohol is settled to 100 milliliters, is the adenosine mother liquor containing 20 μ g/ml adenosines.
Draw 0 milliliter, 0.5 milliliter, 1.0 milliliters, 2.0 milliliters, 3.0 milliliters, 5.0 milliliters, 10 milliliters, adenosine mother liquor respectively and be placed in 10 milliliters of volumetric flasks, with containing 15%(volumn concentration) methanol aqueous solution of methyl alcohol is settled to scale, then use 0.45 μm of filtering with microporous membrane, get filtrate and carry out efficient liquid phase chromatographic analysis.
Chromatographic condition: Agilent C18 analytical column (4.6mm*150mm, 5 μm), mobile phase is the mixed liquor of 10 parts by volume methyl alcohol and 90 parts by volume water, flow velocity 1.00mL/min, sample size 10 μ L, UV detect wavelength 260nm.
Adopt the filtrate production standard curve of each concentration, calibration curve equation is that y=180.67x-7.256, x represent concentration (unit is μ g/ml), and y represents peak area (unit is mAU*s), R
2=1.0000, good in 1-20 mcg/ml scope internal linear relation.
2, the adenosine content of the functional ground rice of pleurotus eryngii that obtains of embodiment 1 and glutinous rice freeze-dried powder is analyzed respectively.
Take the functional ground rice of pleurotus eryngii or glutinous rice freeze-dried powder 1.0g, add 50 milliliters containing 15%(volumn concentration) methanol aqueous solution of methyl alcohol, weigh, ultrasonic extraction 30 minutes, letting cool and weigh, with containing 15%(volumn concentration) methanol aqueous solution of methyl alcohol supplies weight (because moisture may be had to evaporate in ultrasonic leaching process), 0.45 μm of filtering with microporous membrane, get subsequent filtrate, be need testing solution.
Get need testing solution and carry out efficient liquid phase chromatographic analysis, chromatographic condition is the same.
The adenosine content of the functional ground rice of pleurotus eryngii and glutinous rice freeze-dried powder is calculated by calibration curve equation.Adenosine content in the functional ground rice of pleurotus eryngii be 175.64 micrograms/gram.Adenosine content in glutinous rice freeze-dried powder be 14.38 micrograms/gram.
The antioxidation activity of the functional ground rice of embodiment 4, pleurotus eryngii
One, the activity of DPPH free radical is removed
The English full name of DPPH(is 1,1-diphenyl-2-picrylhydrazyl; Chinese full name is 1,1-diphenyl-2-picryl phenylhydrazine) free radical is highly stable a kind of free radical, has strong absorption, when there being antioxidant to exist at 517nm place, the single electron of DPPH is paired and solution colour is shoaled, the antioxidation activity of fading extent reflection antioxidant.DPPH standard items Solarbio, Cat No D0909.Sample to be tested is the functional ground rice of pleurotus eryngii or glutinous rice freeze-dried powder that embodiment 1 obtains.
1, the ethanol extract of sample to be tested removes the activity of DPPH free radical
DPPH standard items absolute ethyl alcohol is made into 2 × 10
-4the solution of M.
10g sample to be tested is added 20ml absolute ethyl alcohol, and soaking at room temperature 30 minutes, gets supernatant, and rotate evaporate to dryness, the dry obtained is ethanol extract.By ethanol extract anhydrous alcohol solution, obtain the solution (concentration is all in the quality of sample to be tested) that concentration is 2mg/ml, 1mg/ml or 0.5mg/ml.
96 orifice plates are adopted to test, 100 μ L sample to be tested solution and 100 μ L DPPH standard solutions are added (sample to be tested hole in 1 hole, 3 multiple holes are set), arrange sample control hole (adds in 1 hole by 100 μ L sample to be tested solution and 100 μ L absolute ethyl alcohols simultaneously, 3 multiple holes are set) and DPPH control wells (100 μ L absolute ethyl alcohols and 100 μ L DPPH standard solutions are added in 1 hole, 3 multiple holes are set), 25 DEG C leave standstill 517nm place after 30 minutes and measure absorbance, are calculated as follows the free radical scavenging activity of test sample:
Free radical scavenging activity=[OD
dPPH control wells-(OD
sample to be tested-OD
sample control hole)]/OD
dPPH control wells× 100%.
Wherein: OD
dPPH control wellsfor the mean value of DPPH control wells OD value; OD
sample to be testedfor the mean value of sample to be tested hole OD value; OD
sample control holefor the mean value of sample controls hole OD value.
EC
50sample to be tested concentration (mg/ml) corresponding in reaction system when value=free radical scavenging activity is 50%.
The results are shown in Table 3.
2, the water extract of sample to be tested removes the activity of DPPH free radical
10g sample to be tested is added 20ml water, and soaking at room temperature 30 minutes, gets supernatant, and rotate evaporate to dryness, the dry obtained is water extract.Water extract anhydrous alcohol solution is diluted, obtains the solution (concentration is all in the quality of sample to be tested) that concentration is 2mg/ml, 1mg/ml or 0.5mg/ml.
The results are shown in Table 3.
Two, reducing power
The potassium ferricyanide [K
3fe (CN)
6] be reduced generation potassium ferrocyanide [K
4fe (CN)
6], potassium ferrocyanide recycling Fe
3+form Prussian blue [Fe
4(Fe (CN)
6)
3], using Prussian blue growing amount as index, measure light absorption value size at 700nm place, light absorption value is higher, then reducing power is stronger.
1, the reducing power of the ethanol extract of sample to be tested
10g sample to be tested is added 20ml absolute ethyl alcohol, and soaking at room temperature 30 minutes, gets supernatant, and rotate evaporate to dryness, the dry obtained is ethanol extract.Ethanol extract is used water-soluble solution, obtains the solution (concentration is all in the quality of sample to be tested) that concentration is 7mg/ml, 3.5mg/ml or 1.75mg/ml.
Get the sample solution of 1ml variable concentrations respectively, add 2.5ml sodium phosphate buffer (0.2mol/L, pH 6.6) and the potassium ferricyanide aqueous solution of 2.5ml 1g/100mL, 50 DEG C of water bath with thermostatic control 20min, add 1ml 10%(volumn concentration after quick cooling) trichloroacetic acid solution and mix, then centrifugal (4 DEG C, 4000r/min, 10min) get supernatant.Get 2.5ml supernatant, add the ferric chloride aqueous solutions (Fresh) of 2.5ml distilled water and 0.5ml 0.1g/100mL, 25 DEG C of standing 10min, measure light absorption value at wavelength 700nm place.Replace 1ml sample solution with 1ml water, carry out above-mentioned experiment, be blank group.
Light absorption value × 100% of percent reduction (%)=(light absorption value of the light absorption value-blank of sample solution)/blank.
EC
50sample to be tested concentration (mg/ml) corresponding in reaction system when value=percent reduction is 50%.
The results are shown in Table 3.
2, the water extract of sample to be tested removes the activity of DPPH free radical
10g sample to be tested is added 20ml water, and soaking at room temperature 30 minutes, gets supernatant, and rotate evaporate to dryness, the dry obtained is water extract.Water extract is used water dissolved dilution, obtain the solution (concentration is all in the quality of sample to be tested) that concentration is 7mg/ml, 3.5mg/ml or 1.75mg/ml.
Test method is with step 1.
The results are shown in Table 3.
Three, sequestering power
Fe
2+the compound formed with Ferrozine has strong light absorption in 562nm, and light absorption value is lower, represents stronger to the sequestering power of iron ion.
10g sample to be tested is added 20ml absolute ethyl alcohol, and soaking at room temperature 30 minutes, gets supernatant, and rotate evaporate to dryness, the dry obtained is ethanol extract.Ethanol extract is used water dissolved dilution, obtain the solution (concentration is all in the quality of sample to be tested) that concentration is 5mg/ml, 2.5mg/ml or 1.25mg/ml.
Get the sample solution 1ml of variable concentrations respectively, add 3.7ml deionized water and 0.1ml 2mmol/L FeCl
2the aqueous solution, adds the 0.2ml 5mmol/L Ferrozine aqueous solution again after 30 seconds, 25 DEG C leave standstill 10 minutes, measure light absorption value in 562nm place.1ml sample solution is replaced to carry out above-mentioned experiment, as blank by 1ml deionized water.The sequestering power of ferrous ion is calculated as follows:
Light absorption value × 100% of chelation percent (%)=(light absorption value of the light absorption value-sample solution of blank)/blank.
EC
50sample to be tested concentration (mg/ml) corresponding in reaction system during value=chelation percent 50%.
The results are shown in Table 3.
2, the water extract of sample to be tested removes the activity of DPPH free radical
10g sample to be tested is added 20ml water, and soaking at room temperature 30 minutes, gets supernatant, and rotate evaporate to dryness, the dry obtained is water extract.Water extract is used water dissolved dilution, obtain the solution (concentration is all in the quality of sample to be tested) that concentration is 5mg/ml, 2.5mg/ml or 1.25mg/ml.
Test method is with step 1.
The results are shown in Table 3.
The antioxidation of the functional ground rice of table 3 pleurotus eryngii
The ethanol extract of the functional ground rice of pleurotus eryngii and water extract show very strong ferrous ion reducing power, its ethanol extract potassium ferricyanide reducing power EC
50be less than 0.25 mg/ml.The ethanol extract of the functional ground rice of pleurotus eryngii and water extract also show very strong ferrous ion sequestering power, are respectively 0.31 ± 0.03 mg/ml and 0.47 ± 0.07 mg/ml.
The preparation of the functional ground rice of embodiment 5, pleurotus eryngii and performance evaluation
One, the preparation of the functional ground rice of pleurotus eryngii
1, the preparation of pleurotus eryngii functional rice meal beetle
(1) preculture
With the step 1 of embodiment 1.
(2) seed liquor is prepared
With the step 2 of embodiment 1.
(3) solid fermentation
Get the seed liquor that 5 milliliters of steps (2) obtain, be seeded to solid medium, 25 DEG C of lucifuge quiescent culture 30 days.
Solid medium: be made up of 80g polished rice and 100 ml waters.
(4) post processing
With the step 4 of embodiment 1.
The freeze-dried powder obtained is pleurotus eryngii functional rice meal beetle.
2, the preparation of the functional ground rice second of pleurotus eryngii
(1) preculture
With the step 1 of embodiment 1.
(2) seed liquor is prepared
With the step 2 of embodiment 1.
(3) solid fermentation
Get the seed liquor that 5 milliliters of steps (2) obtain, be seeded to solid medium, 25 DEG C of lucifuge quiescent culture 35 days.
Solid medium: be made up of 80g long-grained nonglutinous rice and 110 ml waters.
(4) post processing
The whole cultivating system completing solid fermentation is carried out successively drying under reduced pressure and pulverizing (80 mesh sieve), the freeze-dried powder obtained is the functional ground rice second of pleurotus eryngii.
3, the preparation of the functional ground rice third of pleurotus eryngii
(1) preculture
With the step 1 of embodiment 1.
(2) seed liquor is prepared
With the step 2 of embodiment 1.
(3) solid fermentation
Get the seed liquor that 5 milliliters of steps (2) obtain, be seeded to solid medium, 25 DEG C of lucifuge quiescent culture 50 days.
Solid medium: be made up of the 80g seed of Job's tears and 120 ml waters.
(4) post processing
With the step 4 of embodiment 1.
The freeze-dried powder obtained is the functional ground rice third of pleurotus eryngii.
Two, the Analysis of Nutritive Composition of the functional ground rice of pleurotus eryngii
Method is with embodiment 2.
Pleurotus eryngii functional rice meal beetle: the content of total amino acid is 502.28 milligrams/gram, the content of essential amino acid is 122.38 milligrams/gram, the content of reduced sugar is 35.89 milligrams/gram, the content of total polyphenols is 4.14 milligrams/gram, the content of total starches is 17.51 milligrams/gram, the content of protein is 14.26 milligrams/gram, the content of adenosine be 168.64 micrograms/gram.
The functional ground rice second of pleurotus eryngii: the content of total amino acid is 562.43 milligrams/gram, the content of essential amino acid is 162.89 milligrams/gram, the content of reduced sugar is 40.05 milligrams/gram, the content of total polyphenols is 4.12 milligrams/gram, the content of total starches is 21.34 milligrams/gram, the content of protein is 14.22 milligrams/gram, the content of adenosine be 171.52 micrograms/gram.
The functional ground rice third of pleurotus eryngii: the content of total amino acid is 416.47 milligrams/gram, the content of essential amino acid is 121.53 milligrams/gram, the content of reduced sugar is 41.94 milligrams/gram, the content of total polyphenols is 2.92 milligrams/gram, the content of total starches is 23.51 milligrams/gram, the content of protein is 13.45 milligrams/gram, the content of adenosine be 143.24 micrograms/gram.
Three, the antioxidation activity of the functional ground rice of pleurotus eryngii
Method is with embodiment 4.
The results are shown in Table 4.
The antioxidation of the functional ground rice of table 4 pleurotus eryngii
Claims (1)
1. prepare a method for pleurotus eryngii food, comprise the steps: pleurotus eryngii to carry out solid fermentation, obtain pleurotus eryngii food; The culture medium that described solid fermentation adopts is made up of 80g base material and 80-120 ml water; Described base material is glutinous rice or long-grained nonglutinous rice; The condition of culture of described solid fermentation is 25 DEG C of lucifuge quiescent culture 35-40 days; The step of described solid fermentation is: the seed liquor of described pleurotus eryngii is seeded to described solid fermentation culture medium, then carries out described solid fermentation; In the step of described solid fermentation, the seed liquor of pleurotus eryngii described in 5ml is seeded to described solid fermentation culture medium; The preparation method of described seed liquor is as follows: by pleurotus eryngii mycelium inoculation to fluid nutrient medium, and 25 DEG C of lucifuge shaken cultivation 7-10 days, obtain OD
600nmthe seed liquor of=0.75;
The formula of described fluid nutrient medium is: 4 grams of glucose, 10 grams of malt extracts, 4 grams of yeast extracts are water-soluble, is settled to 1 liter with water;
Also comprise in described method and the product of described solid fermentation is carried out dry step; Described drying is freeze drying and/or drying under reduced pressure; Also comprise in described method and dried product is carried out broken step;
Described pleurotus eryngii to be CGMCC deposit number be 5.775 bacterial strain.
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| CN1696280A (en) * | 2004-05-10 | 2005-11-16 | 杨发国 | Technique for planting north aweto in rice bag in high efficiency |
| CN102090268A (en) * | 2010-12-18 | 2011-06-15 | 福建嘉田农业开发有限公司 | Edible fungus strain liquefying inoculation method utilizing grain culture medium as matrix |
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| 发酵料栽培杏鲍菇;孟庆国;《新农业》;20111231(第2期);55-56 * |
| 四个杏鲍菇品种的氨基酸分析与比较;宋爱荣等;《菌物研究》;20051231;第3卷(第4期);11-14 * |
| 杏鲍菇速溶即食营养保健麦片的研制;王新风等;《中国食用菌》;20021231;第21卷(第3期);摘要、第4.4节 * |
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