CN110591865A - Selenium-rich cordyceps militaris wine with liver protection function and preparation method thereof - Google Patents
Selenium-rich cordyceps militaris wine with liver protection function and preparation method thereof Download PDFInfo
- Publication number
- CN110591865A CN110591865A CN201910920127.8A CN201910920127A CN110591865A CN 110591865 A CN110591865 A CN 110591865A CN 201910920127 A CN201910920127 A CN 201910920127A CN 110591865 A CN110591865 A CN 110591865A
- Authority
- CN
- China
- Prior art keywords
- selenium
- cordyceps militaris
- wine
- rich cordyceps
- rich
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 229910052711 selenium Inorganic materials 0.000 title claims abstract description 224
- 239000011669 selenium Substances 0.000 title claims abstract description 224
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 title claims abstract description 220
- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 167
- 235000014101 wine Nutrition 0.000 title claims abstract description 130
- 210000004185 liver Anatomy 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 claims abstract description 44
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 37
- 238000000605 extraction Methods 0.000 claims abstract description 33
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 claims abstract description 25
- 238000002156 mixing Methods 0.000 claims abstract description 23
- 239000002126 C01EB10 - Adenosine Substances 0.000 claims abstract description 22
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 229960005305 adenosine Drugs 0.000 claims abstract description 22
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 claims abstract description 22
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000002994 raw material Substances 0.000 claims abstract description 11
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 claims abstract description 10
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 claims abstract description 10
- 235000013734 beta-carotene Nutrition 0.000 claims abstract description 10
- 239000011648 beta-carotene Substances 0.000 claims abstract description 10
- 229960002747 betacarotene Drugs 0.000 claims abstract description 10
- 238000000034 method Methods 0.000 claims abstract description 10
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 claims abstract description 10
- 239000011550 stock solution Substances 0.000 claims description 49
- 238000002386 leaching Methods 0.000 claims description 47
- 239000006228 supernatant Substances 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 20
- 238000001914 filtration Methods 0.000 claims description 16
- 239000000843 powder Substances 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 12
- 238000002137 ultrasound extraction Methods 0.000 claims description 9
- 238000002791 soaking Methods 0.000 claims description 8
- 238000004321 preservation Methods 0.000 claims description 6
- 238000012364 cultivation method Methods 0.000 claims description 4
- 235000013305 food Nutrition 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 239000000047 product Substances 0.000 claims description 4
- 238000007873 sieving Methods 0.000 claims description 4
- 238000012258 culturing Methods 0.000 claims description 3
- 238000001035 drying Methods 0.000 claims description 3
- 239000003153 chemical reaction reagent Substances 0.000 claims description 2
- 241000190633 Cordyceps Species 0.000 abstract description 7
- 206010067125 Liver injury Diseases 0.000 abstract description 7
- 125000003748 selenium group Chemical group *[Se]* 0.000 abstract description 7
- 230000000975 bioactive effect Effects 0.000 abstract description 5
- 239000000126 substance Substances 0.000 abstract description 5
- 150000001875 compounds Chemical group 0.000 abstract description 2
- 231100000234 hepatic damage Toxicity 0.000 abstract description 2
- 230000008818 liver damage Effects 0.000 abstract description 2
- 229940091258 selenium supplement Drugs 0.000 description 186
- 235000013339 cereals Nutrition 0.000 description 44
- 239000000243 solution Substances 0.000 description 15
- 241000699670 Mus sp. Species 0.000 description 13
- 230000000694 effects Effects 0.000 description 12
- 235000021466 carotenoid Nutrition 0.000 description 8
- 150000001747 carotenoids Chemical class 0.000 description 8
- 239000013543 active substance Substances 0.000 description 7
- 238000004090 dissolution Methods 0.000 description 7
- 230000006870 function Effects 0.000 description 7
- 238000011282 treatment Methods 0.000 description 7
- 230000001476 alcoholic effect Effects 0.000 description 6
- 206010061218 Inflammation Diseases 0.000 description 5
- 230000004054 inflammatory process Effects 0.000 description 5
- 238000009210 therapy by ultrasound Methods 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 238000011049 filling Methods 0.000 description 4
- 230000002538 fungal effect Effects 0.000 description 4
- 231100000753 hepatic injury Toxicity 0.000 description 4
- 210000000265 leukocyte Anatomy 0.000 description 4
- 210000002381 plasma Anatomy 0.000 description 4
- 108020002663 Aldehyde Dehydrogenase Proteins 0.000 description 3
- 241000233866 Fungi Species 0.000 description 3
- 206010028980 Neoplasm Diseases 0.000 description 3
- 210000004369 blood Anatomy 0.000 description 3
- 239000008280 blood Substances 0.000 description 3
- 230000006378 damage Effects 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 230000036039 immunity Effects 0.000 description 3
- 230000003859 lipid peroxidation Effects 0.000 description 3
- 229920001282 polysaccharide Polymers 0.000 description 3
- 239000005017 polysaccharide Substances 0.000 description 3
- 150000003254 radicals Chemical class 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000002798 spectrophotometry method Methods 0.000 description 3
- 239000011573 trace mineral Substances 0.000 description 3
- 235000013619 trace mineral Nutrition 0.000 description 3
- FDKWRPBBCBCIGA-REOHCLBHSA-N (2r)-2-azaniumyl-3-$l^{1}-selanylpropanoate Chemical compound [Se]C[C@H](N)C(O)=O FDKWRPBBCBCIGA-REOHCLBHSA-N 0.000 description 2
- FDKWRPBBCBCIGA-UWTATZPHSA-N D-Selenocysteine Natural products [Se]C[C@@H](N)C(O)=O FDKWRPBBCBCIGA-UWTATZPHSA-N 0.000 description 2
- 102000001554 Hemoglobins Human genes 0.000 description 2
- 108010054147 Hemoglobins Proteins 0.000 description 2
- 240000007594 Oryza sativa Species 0.000 description 2
- 235000007164 Oryza sativa Nutrition 0.000 description 2
- RJFAYQIBOAGBLC-BYPYZUCNSA-N Selenium-L-methionine Chemical compound C[Se]CC[C@H](N)C(O)=O RJFAYQIBOAGBLC-BYPYZUCNSA-N 0.000 description 2
- RJFAYQIBOAGBLC-UHFFFAOYSA-N Selenomethionine Natural products C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 description 2
- 230000003110 anti-inflammatory effect Effects 0.000 description 2
- 125000003118 aryl group Chemical group 0.000 description 2
- 238000001391 atomic fluorescence spectroscopy Methods 0.000 description 2
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000033228 biological regulation Effects 0.000 description 2
- 238000004820 blood count Methods 0.000 description 2
- 201000011510 cancer Diseases 0.000 description 2
- 230000007123 defense Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 230000035622 drinking Effects 0.000 description 2
- 239000000796 flavoring agent Substances 0.000 description 2
- 235000019634 flavors Nutrition 0.000 description 2
- 230000006872 improvement Effects 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 210000005229 liver cell Anatomy 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 238000007254 oxidation reaction Methods 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 238000010298 pulverizing process Methods 0.000 description 2
- 230000001603 reducing effect Effects 0.000 description 2
- 235000009566 rice Nutrition 0.000 description 2
- 229940065287 selenium compound Drugs 0.000 description 2
- 150000003343 selenium compounds Chemical class 0.000 description 2
- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 description 2
- 235000016491 selenocysteine Nutrition 0.000 description 2
- 229940055619 selenocysteine Drugs 0.000 description 2
- 229960002718 selenomethionine Drugs 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 241000272814 Anser sp. Species 0.000 description 1
- 241000235349 Ascomycota Species 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 208000024172 Cardiovascular disease Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 206010016654 Fibrosis Diseases 0.000 description 1
- 229940123457 Free radical scavenger Drugs 0.000 description 1
- 206010019663 Hepatic failure Diseases 0.000 description 1
- 206010019668 Hepatic fibrosis Diseases 0.000 description 1
- 206010019670 Hepatic function abnormal Diseases 0.000 description 1
- 208000005176 Hepatitis C Diseases 0.000 description 1
- 102000004895 Lipoproteins Human genes 0.000 description 1
- 108090001030 Lipoproteins Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 206010039921 Selenium deficiency Diseases 0.000 description 1
- 240000006394 Sorghum bicolor Species 0.000 description 1
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 1
- 235000021307 Triticum Nutrition 0.000 description 1
- 244000098338 Triticum aestivum Species 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 240000008042 Zea mays Species 0.000 description 1
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 1
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 231100000439 acute liver injury Toxicity 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 230000001174 ascending effect Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000036983 biotransformation Effects 0.000 description 1
- 238000013124 brewing process Methods 0.000 description 1
- 210000005252 bulbus oculi Anatomy 0.000 description 1
- 230000001612 cachectic effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 210000000170 cell membrane Anatomy 0.000 description 1
- 210000003850 cellular structure Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007882 cirrhosis Effects 0.000 description 1
- 208000019425 cirrhosis of liver Diseases 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 235000009508 confectionery Nutrition 0.000 description 1
- 235000005822 corn Nutrition 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 230000003814 hair luster Effects 0.000 description 1
- 210000003494 hepatocyte Anatomy 0.000 description 1
- 238000001676 hydride generation atomic fluorescence spectroscopy Methods 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 201000007270 liver cancer Diseases 0.000 description 1
- 208000019423 liver disease Diseases 0.000 description 1
- 231100000835 liver failure Toxicity 0.000 description 1
- 208000007903 liver failure Diseases 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 208000014018 liver neoplasm Diseases 0.000 description 1
- 210000005228 liver tissue Anatomy 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- 239000002398 materia medica Substances 0.000 description 1
- 230000006996 mental state Effects 0.000 description 1
- 239000011259 mixed solution Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 210000004940 nucleus Anatomy 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000000643 oven drying Methods 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 235000021485 packed food Nutrition 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 230000035790 physiological processes and functions Effects 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 239000002516 radical scavenger Substances 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 229960001471 sodium selenite Drugs 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 238000000527 sonication Methods 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 235000020097 white wine Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12G—WINE; PREPARATION THEREOF; ALCOHOLIC BEVERAGES; PREPARATION OF ALCOHOLIC BEVERAGES NOT PROVIDED FOR IN SUBCLASSES C12C OR C12H
- C12G3/00—Preparation of other alcoholic beverages
- C12G3/04—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs
- C12G3/05—Preparation of other alcoholic beverages by mixing, e.g. for preparation of liqueurs with health-improving ingredients, e.g. flavonoids, flavones, polyphenols or polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Polymers & Plastics (AREA)
- Mycology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Animal Husbandry (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention provides selenium-rich cordyceps militaris wine with a liver protection function and a preparation method thereof. The selenium-rich cordyceps militaris wine is prepared by taking selenium-rich cordyceps militaris as a raw material, preparing a selenium-rich cordyceps militaris extract by an alcohol extraction method, and then blending the selenium-rich cordyceps militaris extract with pure grain wine. The selenium-rich cordyceps militaris contains 78-750 mu g/L of organic selenium, 1000mg/L of cordycepin, 1500mg/L of adenosine, 0.7-7.5mg/mL of cordycepic acid, 0.5-26.9mg/mL of beta-carotene and 15-68 degrees of alcohol. The selenium leached from the selenium-rich cordyceps militaris sporocarp is mainly selenium in an organic selenium form, is easy to absorb and higher in safety, the method can economically and efficiently obtain the selenium-rich cordyceps militaris wine with stable selenium content, simultaneously contains cordyceps bioactive substances such as cordycepin, adenosine and the like, and reduces liver damage caused by alcohol by utilizing the compound function of the selenium and the cordyceps militaris bioactive substances.
Description
Technical Field
The invention relates to selenium-rich cordyceps militaris wine with a liver protection function and a preparation method thereof.
Background
Selenium is one of essential trace elements for human body, and selenium deficiency can cause various diseases of human body. A large number of researches show that selenium has various physiological functions of improving immunity, resisting inflammation, resisting cancer, resisting fatigue and the like. Inorganic selenium is the main existing form in the natural environment, and organic selenium has higher biological activity and is easy to digest and absorb by animals. In addition, research proves that the organic selenium compound is also an important factor forming an endogenous defense system of an organism, so that oxidative damage of free radicals, active oxygen and active oxygen free radicals in vivo to cell components such as cell membranes, lipids, lipoproteins, DNA and the like is reduced, and the organic selenium compound is closely related to the occurrence of various diseases such as inflammation, cancer, cardiovascular disease and the like.
Cordyceps militaris (Cordyceps militaris) is also called Cordyceps militaris, and belongs to fungi of genus Cordyceps of family Clavipitaceae of phylum Ascomycota. The compendium of Xinhua materia Medica calls cordyceps militaris 'whole grass': sweet in flavor and neutral in nature. The fruiting body of Cordyceps militaris contains various functional active substances, including cordycepin, Cordyceps polysaccharide, cordycepic acid, adenosine, etc. A large number of researches show that the cordyceps militaris sporocarp has pharmacological actions of resisting virus, inflammation, oxidation, aging, tumor, fatigue, immunity enhancement, kidney and liver protection and the like, and particularly has good protection and treatment effects on various liver diseases such as alcoholic liver injury, non-alcoholic liver injury, hepatitis C, hepatic fibrosis, cirrhosis, liver failure, liver cancer and the like.
The contents of cordycepin, adenosine, cordycepic acid, cordyceps polysaccharide and the like in the selenium-rich cordyceps militaris sporocarp are higher than those of the common cordyceps militaris sporocarp, and selenium mainly exists in an organic selenium form, and can promote the enhancement of the antioxidant activity of the cordyceps militaris sporocarp.
At present, the production process of selenium-containing wine mainly obtains the selenium-rich wine by adding sodium selenite in the wine brewing process or adding selenium-rich agricultural product raw materials, and the process has the following problems: the selenium yield is low, and the selenium-containing wine has high cost, more impurities, unstable selenium content and alcoholic strength, high inorganic selenium residual quantity and the like.
Disclosure of Invention
The invention aims to provide selenium-rich cordyceps militaris wine with a liver protecting function and a preparation method thereof.
In order to achieve the purpose of the invention, on the first aspect, the invention provides selenium-rich cordyceps militaris wine with a liver protection function, wherein the selenium-rich cordyceps militaris is used as a raw material, a selenium-rich cordyceps militaris extract is prepared by an alcohol extraction method, and then the selenium-rich cordyceps militaris extract is prepared by blending with pure grain wine (pure grain white wine).
The selenium-rich cordyceps militaris contains 78-750 mu g/L of organic selenium, 1000mg/L of cordycepin, 1500mg/L of adenosine, 0.7-7.5mg/mL of cordycepic acid, 0.5-26.9mg/mL of beta-carotene and 15-68 degrees of alcohol.
The extraction reagent used in the alcohol extraction method is pure grain wine.
In a second aspect, the invention provides a selenium-rich cordyceps militaris extract, and a preparation method of the selenium-rich cordyceps militaris extract comprises the following steps:
A. obtaining selenium-rich cordyceps militaris: culturing Cordyceps militaris CP1(Cordyceps militaris CP1) (high selenium-resistant Cordyceps militaris strain) with a preservation number of CCTCC NO of M2019671 in a culture medium added with nano selenium by adopting a cultivation method of selenium-rich Cordyceps militaris disclosed in CN201710433450.3 to obtain selenium-rich Cordyceps militaris with organic selenium content of 120-140 mg/kg;
B. preparation of selenium-rich Cordyceps militaris leaching liquor
B1, drying the selenium-rich cordyceps militaris at 45 ℃, crushing the selenium-rich cordyceps militaris, sieving the crushed selenium-rich cordyceps militaris with a sieve of 150 meshes and 200 meshes to obtain ultramicro selenium-rich cordyceps militaris sporocarp powder, soaking the ultramicro selenium-rich cordyceps militaris sporocarp powder in pure grain wine with the temperature of 15-68 ℃ according to the material-liquid ratio of 2.5-7.5%, stirring and uniformly mixing,
ultrasonic extracting at 80-100Hz for 15-45min, standing at 15-20 deg.C for 9-12h, filtering and clarifying to obtain supernatant (naturally standing in a clarifying tank, filtering to obtain wine base extractive solution, and clarifying at 15-20 deg.C);
b2, adding 68-degree pure grain wine with the same volume as that of the 15-68-degree pure grain wine used in B1 into the residue after filtration of B1, stirring and uniformly mixing, carrying out ultrasonic extraction at 80-100HZ for 15-45min, standing for 9-12h at 15-20 ℃, filtering and clarifying to obtain a supernatant stock solution;
b3, repeating the step B23;
C. and (4) mixing the supernatant stock solutions obtained in the step (B), and obtaining the selenium-rich cordyceps militaris leaching liquor, namely the selenium-rich cordyceps militaris extract.
The alcohol degree in the leaching stock solution is 40-45 degrees. The selenium extracted from the selenium-rich cordyceps militaris sporocarp is mainly selenium in an organic selenium form, the total selenium content is 1100-1500 mu g/L, and the selenium mainly exists in two organic selenium forms of selenomethionine and selenocysteine. The cordycepin content in the leaching stock solution is 1500-2000mg/L, the adenosine content is 1800-3000mg/L, the cordycepic acid content is 10-15mg/mL, and the beta-carotene content is 7.6-53.7 mg/mL.
In a third aspect, the invention provides application of the selenium-rich cordyceps militaris extract in the fields of food, health-care products and feed.
In a fourth aspect, the invention provides a preparation method of the selenium-rich cordyceps militaris wine, which comprises the following steps: blending the selenium-rich cordyceps militaris extract and pure grain wine in proportion to obtain the selenium-rich cordyceps militaris wine. The selenium-rich Cordyceps militaris wine has bright color of yellow goose, soft and mellow taste, and stable alcoholic strength of 15-68 deg.
By the technical scheme, the invention at least has the following advantages and beneficial effects:
the selenium-rich cordyceps militaris wine provided by the invention contains various active substances, including cordyceps polysaccharide, various amino acids, cordycepin, adenosine, cordycepic acid, carotenoid, trace element selenium and various other trace elements.
And (II) under the condition that the taste and the flavor of the pure grain wine are not changed, the selenium-rich cordyceps militaris wine which contains organic selenium and various cordyceps active substances and is bright in color and luster is produced, and drinking the selenium-rich cordyceps militaris wine can provide a new selenium supplementing way for people, reduce the damage of alcohol to the liver and play a role in protecting the liver.
And thirdly, the selenium leached from the selenium-rich cordyceps militaris sporocarp is mainly selenium in an organic selenium form, is easy to absorb and has higher safety, the method can economically and efficiently obtain the selenium-rich cordyceps militaris wine with stable selenium content, simultaneously contains cordycepin, adenosine and other cordyceps bioactive substances, and reduces liver damage caused by alcohol by utilizing the compound function of the selenium and the cordyceps militaris bioactive substances.
Drawings
FIG. 1 is a schematic view of the production process of selenium-rich Cordyceps militaris wine.
FIG. 2 is a diagram of ultramicro bacterial powder of selenium-rich cordyceps militaris fruiting body in embodiment 1 of the invention.
FIG. 3 shows the selenium-enriched Cordyceps militaris wine obtained by leaching pure grain wine with different alcohol concentrations in example 2 of the present invention. Wherein, I to VI respectively represent alcohol concentration of 15 degrees, 25.0 degrees, 40.0 degrees, 46.0 degrees, 68.0 degrees and 96.0 degrees, and Control (contrast) is not soaked in alcohol grain wine and takes 15 degrees as an example.
FIG. 4 shows the dissolution rate of total selenium in selenium-rich Cordyceps militaris by alcohol pure grain wine of different concentrations in example 2 of the present invention. In the figure, different lower case letters indicate significant differences between treatments (P < 0.05).
FIG. 5 shows the dissolution of carotenoids from selenium-rich Cordyceps militaris in example 2 of the present invention with pure grain alcoholic liquors of different concentrations.
FIG. 6 shows the total selenium dissolution from the leaching solution of selenium-rich Cordyceps militaris in example 3 of the present invention at different feed-to-solution ratios. In the figure, different lower case letters indicate significant differences between treatments (P < 0.05).
FIG. 7 shows the leaching solution of selenium-rich Cordyceps militaris in example 3 of the present invention at different feed-liquid ratios.
FIG. 8 shows the total selenium leaching rate of the selenium-rich Cordyceps militaris leachate in example 4 of the present invention at different ultrasound times.
FIG. 9 shows the cordycepic acid content in the Cordyceps militaris leachate at different ultrasonic times in example 4 of the present invention.
FIG. 10 shows the total selenium leaching rate in the leaching solution of selenium-rich Cordyceps militaris at different leaching temperatures in example 5 of the present invention.
FIG. 11 shows the dissolution rate of cordycepic acid in the selenium-rich Cordyceps militaris leaching solution at different leaching temperatures in example 5 of the present invention.
FIG. 12 is a chart of the HPLC-HG-AFS analysis of selenium form in the leaching solution of the selenium-enriched Cordyceps militaris wine in example 6 of the present invention.
FIG. 13 is a mouse liver injury slice after drinking selenium-rich Cordyceps militaris wine in example 8 of the present invention.
Detailed Description
The invention provides the selenium-rich cordyceps militaris wine which is low in cost, stable in selenium content and alcohol content and contains various active substance components, wherein the content of organic selenium in the selenium-rich cordyceps militaris wine is 78-750 mu g/L, the content of cordycepin is 115-1000mg/L, the content of adenosine is 128-1500mg/L, the content of cordycepic acid is 0.7-7.5mg/mL, the content of beta-carotene is 7.6-53.7mg/mL, and the alcohol content is 15-68 ℃.
The preparation method of the selenium-rich cordyceps militaris wine comprises the following steps:
the cultivation method of selenium-rich cordyceps militaris is disclosed in CN 201710433450.3. Culturing high selenium-resistant Cordyceps militaris strain (Cordyceps militaris CP1, preservation number is CCTCC NO: M2019671) in culture medium with nano-selenium to obtain selenium-rich Cordyceps militaris fruiting body with organic selenium content of 120-140 mg/kg.
The preparation method of selenium-rich Cordyceps militaris wine with selenium-rich Cordyceps militaris fruiting body as raw material comprises (as shown in figure 1):
(1) preparing selenium-rich cordyceps militaris leaching solution:
1) processing selenium-rich cordyceps militaris raw materials: drying and crushing the selenium-rich cordyceps militaris dry sample, then sieving with a 200-mesh sieve to obtain ultramicro selenium-rich cordyceps militaris fruiting body fungus powder, soaking the ultramicro selenium-rich cordyceps militaris fruiting body fungus powder in 15-degree pure grain wine according to the material-liquid ratio of 2.5-7.5%, uniformly stirring, carrying out 80HZ ultrasonic extraction for 30-45min, standing for 9h at 15-20 ℃, and filtering and clarifying to obtain a supernatant stock solution;
2) adding the residue obtained in step 1) into 68-degree pure aromatic wine with the same volume, stirring, mixing, ultrasonic extracting at 80HZ for 30-45min, standing at 15-20 deg.C for 9h, filtering, and clarifying to obtain supernatant stock solution; repeating for 3 times;
3) mixing the supernatants obtained in 1) and 2) to obtain a leaching stock solution, analyzing selenium content by atomic fluorescence spectrometry, measuring cordycepic acid by ultraviolet spectrophotometry, and analyzing contents of active substances such as cordycepin and adenosine by high performance liquid chromatography for later use.
(2) Preparing the selenium-rich cordyceps militaris wine:
and (2) blending the selenium-rich cordyceps militaris leaching stock solution obtained in the step (1) according to a proportion by using 15-degree, 58-degree and 68-degree pure grain wine to obtain the selenium-rich cordyceps militaris wine, wherein the organic selenium content of the selenium-rich cordyceps militaris wine is 78-750 mu g/L, the cordycepin content is 115 mg/L, the adenosine content is 128-1500mg/L, the cordycepic acid content is 0.7-7.5mg/mL, the beta-carotene content is 0.5-26.9mg/mL, and the alcohol content is 15-68-degree.
The pure grain wine is used for extracting organic selenium and bioactive substances such as cordycepin, adenosine, cordycepic acid, carotenoid and the like from the selenium-rich cordyceps militaris sporocarp. The obtained selenium-rich Cordyceps militaris wine has yellow and clear color, soft and mellow taste, and stable alcoholic strength of 15-68 deg.
Placing the obtained mixed solution of selenium-rich Cordyceps militaris and pure grain Chinese liquor into a clarifying tank, naturally standing, and filtering to obtain liquor base leaching clear solution, wherein the temperature is controlled below 15-20 deg.C during clarification.
The high selenium-resistant Cordyceps militaris strain, Cordyceps militaris CP1(Cordyceps militaris CP1), related in the invention is currently deposited in China center for type culture Collection, and the address is as follows: wuhan, Wuhan university, post code 430072, preservation number CCTCC NO: M2019671, preservation date 2019, 8 months and 26 days.
The following examples are intended to illustrate the invention but are not intended to limit the scope of the invention. Unless otherwise specified, the technical means used in the examples are conventional means well known to those skilled in the art, and the raw materials used are commercially available products.
The pure grain wine used in the following examples is brewed from grains (sorghum, corn, rice, glutinous rice or wheat) as raw materials by a distiller's yeast fermentation method in the traditional white spirit brewing technology.
Example 1 obtaining of selenium-enriched Cordyceps militaris raw Material
By adopting the cultivation method of selenium-rich cordyceps militaris disclosed in CN201710433450.3, a high-selenium-resistant cordyceps militaris strain (cordyceps militaris CP1 with the preservation number of CCTCC NO: M2019671) is cultured in a culture medium added with nano-selenium, and selenium cordyceps militaris fruiting bodies with the organic selenium content of 120-140mg/kg are obtained. Collecting selenium-rich Cordyceps militaris fruiting body obtained by biotransformation, oven drying at 45 deg.C, adding into a pulverizing device, pulverizing, and sieving with 200 mesh sieve to obtain ultramicro powder (figure 2) of selenium-rich Cordyceps militaris fruiting body for subsequent extraction.
Example 2 preparation of selenium-rich Cordyceps militaris wine stock solution at different alcohol concentrations
Soaking the ultramicro powder of the selenium-rich cordyceps militaris sporocarp obtained in the example 1 in six different pure grain wines with alcohol degrees of 15-96 degrees according to the material-liquid ratio of 2.5%, stirring and uniformly mixing, carrying out ultrasonic extraction for 10min, standing for 9h at 15-20 ℃, filtering and clarifying to obtain a supernatant stock solution, and repeating for 3 times; the supernatants obtained were combined to obtain the leach liquor (FIG. 3). The wine with five concentrations of 15 degrees, 25 degrees, 40 degrees, 46 degrees and 68 degrees can dissolve 482.9 +/-15.6 mu g/L, 420.1 +/-32.1 mu g/L, 319.9 +/-10.5 mu g/L, 304.1 +/-6.2 mu g/L and 305.6 +/-16.8 mu g/L of total selenium, and the total selenium of 96-degree pure grain wine can only dissolve 104.1 +/-9.5 mu g/L under the conditions (figure 4). The selenium-rich cordyceps militaris sporocarp contains rich carotenoids, the selenium-rich cordyceps militaris wine prepared under different alcohol concentrations has characteristic absorption peaks of three carotenoids at 430nm, 450nm and 480nm, wherein the dissolution rate of beta-carotene (lambda is 450nm) in 68-degree pure grain wine is 69.1mg/mL (Table 1). The ultraviolet spectrophotometric analysis showed that the alcohol was able to dissolve more carotenoids at four concentrations of 40.0 degrees, 46 degrees, 68 degrees and 96 degrees, while the low alcohol concentrations, 15 degrees and 25.0 degrees, were less carotenoids (fig. 5). The 15-degree wine has the highest dissolution rate of cordycepin and adenosine in the selenium-rich cordyceps militaris wine leachate prepared by analyzing pure grain wine with different alcohol concentrations by using high performance liquid chromatography, and the contents of cordycepin and adenosine in the leaching stock solution can reach 1487.6 +/-129.7 mg/L and 1689.7 +/-164.4 mg/L respectively (Table 1).
TABLE 1 extraction amount of cordycepin and adenosine from selenium-rich Cordyceps militaris by pure grain wine with different alcohol concentration
Note: ND is below the detection limit.
Example 3 preparation of selenium-rich Cordyceps militaris wine stock solution at different feed-to-solution ratios
Soaking the ultramicro fungal powder of the selenium-rich cordyceps militaris sporocarp obtained in the example 1 in the 15-degree and 68-degree pure grain wines with better extraction effect obtained in the example 2 according to the material-liquid ratio of 0.1-20%, stirring and uniformly mixing, performing ultrasonic extraction for 30min, standing for 9h at 15-20 ℃, filtering and clarifying to obtain a supernatant stock solution, and repeating for 3 times; mixing the obtained supernatants to obtain a leaching stock solution. As the feed-liquid ratio is increased, the total selenium in the leaching liquor is increased from 152.1 +/-1.9 mu g/L to 3677.8 +/-478.7 mu g/L, and the leaching liquor has a significant ascending trend (figure 6). When the feed-liquid ratio reaches 5%, the total selenium dissolution can reach 771.1 +/-111.0 mu g/L, when the total selenium concentration reaches 1102.5 +/-128.1 mu g/L at 7.5%, when the total selenium concentration reaches 2133.4 +/-278.9 mu g/L at 10.0%, and when the total selenium concentration reaches 3553.1-3677.75 mu g/L under the condition of 15.0% and 20% feed-liquid ratio, the maximum value is reached and no obvious difference exists. Meanwhile, the cordycepic acid content in the leaching solution (figure 7) is remarkably increased along with the increase of the feed-liquid ratio, and reaches 13.4-14.0mg/mL when the feed-liquid ratio reaches 2.5% and 5%. The concentration of cordycepic acid in the leaching solution can reach 11.3-33.3mg/mL along with the increase of the feed-liquid ratio.
Example 4 preparation of selenium-rich Cordyceps militaris wine stock solution at different ultrasonic times
Soaking the ultramicro fungal powder of the selenium-rich cordyceps militaris sporocarp obtained in the example 1 in the 15-degree and 68-degree pure grain wines with better extraction effect obtained in the example 2 according to the feed liquid ratio of 2.5-7.5% in the example 3, stirring and uniformly mixing, respectively carrying out ultrasonic extraction for 15-240min, standing for 9h at 15-20 ℃, filtering and clarifying to obtain a supernatant stock solution, and repeating for 3 times; mixing the obtained supernatants to obtain a leaching stock solution. The total selenium leaching rate of the leaching solution did not change much for different sonication times (fig. 8). By utilizing the material-liquid ratio of 5.0 percent, the total selenium content in the selenium-rich cordyceps militaris wine leaching liquor can reach 1080.0 +/-40.4 mu g/L after 80HZ ultrasonic treatment for 15min, and the total selenium content in the selenium-rich cordyceps militaris wine leaching liquor is not obviously improved along with the increase of ultrasonic treatment time. Meanwhile, with the increase of the ultrasonic time, the content of the cordycepic acid in the selenium-enriched cordyceps militaris wine leaching liquor is not obviously increased. After the ultrasonic treatment for 15min, the cordycepic acid content in the selenium-enriched cordyceps militaris wine leaching liquor reaches 17.2 +/-2.0 mg/mL (figure 9). Therefore, the influence of different leaching time on selenium and active substances in the leaching liquor is not great, so that the optimized condition is combined with the actual operation, and the ultrasonic treatment is carried out for 15min in the leaching process.
Example 5 preparation of selenium-rich Cordyceps militaris wine stock solution at different extraction temperatures
Soaking the ultramicro fungal powder of the selenium-rich cordyceps militaris sporocarp obtained in the example 1 in the 15-degree and 68-degree pure grain wines with better extraction effects obtained in the example 2 according to the material-liquid ratio of better 2.5% -7.5% in the example 3 respectively, stirring and uniformly mixing, carrying out better ultrasonic extraction for 15min in the example 4, standing at 20-90 ℃ for 9h respectively, filtering and clarifying to obtain a supernatant stock solution, and repeating for 3 times; mixing the obtained supernatants to obtain a leaching stock solution. Under the condition of 5.0% material-to-liquid ratio, the total selenium content in the leaching solution of selenium-enriched Cordyceps militaris wine at 20-90 deg.C is 738.8 + -76.1 μ g/L-1094.1 + -19.4 μ g/L (figure 10), and the cordycepic acid content is 11.5 + -13.0 mg/mL-13.9 + -0.5 mg/mL (figure 11). Therefore, different extraction temperatures have no significant influence on the total selenium leaching rate and the cordycepic acid content of the leaching solution, so that the optimization conditions are combined with the actual operation, and the leaching is carried out at room temperature.
Example 6 preparation of selenium-enriched Cordyceps militaris wine stock solution
Soaking the ultramicro fungal powder of the selenium-rich cordyceps militaris sporocarp obtained in the example 1 in the 15-degree and 68-degree pure grain wine with the better extraction effect obtained in the example 2 according to the material-liquid ratio of better 2.5% -7.5% in the example 4, uniformly stirring, carrying out better ultrasonic extraction for 15min in the example 3, standing for 9h at 15-20 ℃ obtained in the example 5, and filtering and clarifying to obtain a supernatant stock solution; adding the residue into 68% pure aromatic wine with the same volume, stirring, mixing, ultrasonic extracting for 15min, standing at 15-20 deg.C for 9 hr, filtering, clarifying to obtain supernatant, and repeating for 3 times; mixing the obtained supernatants to obtain a leaching stock solution, and repeating for 3 times; mixing the obtained supernatants to obtain a leaching stock solution. Analyzing the content and form of organic selenium in the leaching stock solution by using a high performance liquid chromatography-atomic fluorescence spectrometry method; analyzing the content of cordycepin and adenosine by high performance liquid chromatography; analyzing the contents of cordycepic acid and carotenoid by ultraviolet spectrophotometry. After the raw materials and the liquid are subjected to ultrasonic treatment for 15min at the material-liquid ratio of 5.0 percent and are kept stand for 9h at the leaching temperature of 15-20 ℃, the alcohol degree in the leaching stock solution is 40-45 ℃, the total selenium content is 1100-1500 mu g/L, and the raw materials mainly exist in two organic selenium forms of selenomethionine and selenocysteine (figure 12). The cordycepin content in the leaching stock solution is 1500-2000mg/L, the adenosine content is 1800-3000mg/L, and the cordycepic acid content is 10-15 mg/mL.
Example 7 preparation of selenium-enriched Cordyceps militaris wine with different proportions
The leaching stock solution obtained in example 6 was blended with pure grain white spirit of 15 degrees, 58 degrees and 68 degrees. The reference value (NRV) of the nutrient of the food label of selenium specified in the national standard for food safety pre-packaged food nutrition label regulation of GB28050-2011 is 50 mug, and the requirements and conditions for selenium enrichment or selenium content are that the NRV is more than or equal to 15% in each 100mL or more than or equal to 10% in each 420kJ, namely the requirements can indicate that the selenium content in the selenium-enriched or selenium-containing beverage needs to be more than or equal to 75 mug/L. The selenium content in the selenium-rich wine is more than or equal to 75 mu g/L and is consistent with the regulation in the standard of the selenium-rich wine in Shaanxi local standard DB 61T556-2018 in 2018. The prepared selenium-rich cordyceps militaris wine is rich in various active substances such as organic selenium, cordycepin, adenosine and cordycepic acid. According to different proportions in Table 2, selenium-enriched cordyceps militaris wine (Table 2) with the selenium content of 78-750 mu g/L, the cordycepin content of 115-1000mg/L, the adenosine content of 128-1500mg/L, the cordycepic acid content of 0.7-7.5mg/mL, the beta-carotene content of 0.5-26.9mg/mL and the alcohol content of 15-68 degrees can be obtained.
TABLE 2 selenium-enriched Cordyceps militaris wine proportioning Table
*: the organic selenium content in the selenium-enriched cordyceps militaris wine is (the organic selenium content in the extraction stock solution is multiplied by the extraction stock solution part and is multiplied by 0 multiplied by the prepared pure grain wine part)/(the extraction stock solution part and the prepared pure grain wine part);
the cordycepin content in the selenium-enriched cordyceps militaris wine is (the cordycepin content in the extraction stock solution is multiplied by the extraction stock solution part plus 0 multiplied by the prepared pure grain wine part)/(the extraction stock solution part plus the prepared pure grain wine part);
the adenosine content in the selenium-enriched cordyceps militaris wine is (adenosine content in the extraction stock solution is multiplied by the extraction stock solution part and 0 multiplied by the prepared pure grain wine part)/(the extraction stock solution part and the prepared pure grain wine part);
the content of the cordycepic acid in the selenium-enriched cordyceps militaris wine is (the content of the cordycepic acid in the extraction stock solution is multiplied by the part of the extraction stock solution and is multiplied by 0 multiplied by the part of the prepared pure grain wine)/(the part of the extraction stock solution and the part of the prepared pure grain wine);
the content of beta-carotene in the selenium-enriched cordyceps militaris wine is (the content of beta-carotene in the extraction stock solution is multiplied by the part of the extraction stock solution plus 0 multiplied by the part of the prepared pure grain wine)/(the part of the extraction stock solution plus the part of the prepared pure grain wine);
the alcohol content (v/v,%) in the selenium-enriched cordyceps militaris wine is (alcohol content in the extraction stock solution is multiplied by the extraction stock solution fraction + the blended wine alcohol content is multiplied by the blended pure grain wine fraction)/(the extraction stock solution fraction + the blended pure grain wine fraction).
Example 8 influence of selenium-enriched Cordyceps militaris wine with different proportions on liver injury of mice
A mouse (20g, male ICR) is intragastrically filled with 6mL/kg daily to obtain 18 (i.e. the mixture ratio of 18 in Table 2) selenium-rich Cordyceps militaris wine and 6mL/kg common pure grain wine with the same alcohol content, and the intragastrically filled wine is continuously filled for 30 days to analyze liver function index of the mouse. After the stomach is continuously filled with 6mL/kg of wine for one week, the mice in the stomach-filling group filled with the common pure grain wine are generally poor in state, dry and lusterless in body hair, cachectic and poor in activity, and the weight of the mice is lower than that of the mice in the stomach-filling group filled with the selenium-enriched cordyceps militaris wine. Compared with the stomach-filling group of the common pure grain wine, the mice in the stomach-filling group of the selenium-enriched cordyceps militaris wine have better mental state and hair luster, and have normal activities. In the stomach group filled with the common pure grain wine, the liver cells are swollen, the liver cell nuclei are seriously shrunk, and fat cavities are formed; the selenium-rich Cordyceps militaris wine group cells were arranged neatly, and other states of hepatocytes were close to those of the blank control group (FIG. 13). Meanwhile, carrying out eyeball picking and blood taking on the mice in the experimental group, separating plasma and carrying out detection; quickly dislocating and killing the mice, weighing the liver, and calculating the liver index; taking a proper amount of prepared blood plasma to measure the activity of ADH, ALT and ALDH enzymes; accurately weighing 0.5g of the same part of the liver, and mixing the parts in a ratio of 1: 9(m/V) physiological saline is prepared into liver homogenate. SOD activity, CAT activity, selenium content, MDA content and GSH content in liver tissue are respectively measured according to the method of the kit specification. By comparing the conventional indexes of the blood of the mice (table 3), the blank control group has obviously increased hemoglobin concentration and white blood cell number compared with the model group; compared with a model group, the selenium-rich cordyceps militaris wine group has the advantage that the hemoglobin concentration is restored to a normal level. Leukocytes are immune cells of the body and are the main markers reflecting the immune and anti-inflammatory functions of the body. When the body becomes inflamed or the liver receives damage, the white blood cell count increases, and as the inflammation subsides, the damage is repaired and the white blood cell count decreases. These indicators indicate that alcohol causes impaired liver function, which in turn leads to disturbances in other physiological activities of the body. The selenium-rich Cordyceps militaris wine can ensure immunity and anti-inflammatory function of organism, and reduce inflammation. The ALT content in the model group is obviously higher than that of the blank control group, and the selenium-rich cordyceps militaris wine group can obviously reduce the ALT content and has no obvious difference with the control group; the ALDH and ADH contents in the plasma of the mice have no significant difference under different treatment conditions, wherein the activities of the two enzymes of the selenium-enriched cordyceps militaris wine group tend to be of a blank control group (Table 4). When the liver is damaged, the content of ADH and ALT in the serum is increased. This shows that the selenium-rich Cordyceps militaris wine has strong scavenging ability for free radicals in mice and can protect liver. In the model group, the MDA content in the liver of the mouse is significantly higher than that of the control group, while the selenium-enriched cordyceps militaris wine group has a trend of reducing MDA, but is not significant compared with the model group, and the MDA content is close to that of the blank control group (table 5). The method proves that the alcohol induces the organism to generate obvious lipid peroxidation, the selenium-rich cordyceps militaris wine group has a remarkable MDA reducing effect, the lipid peroxidation level in the organism is reduced to some extent, and the selenium-rich cordyceps militaris wine group has an obvious protective effect on the lipid peroxidation caused by the alcohol. The model group alcohol causes GSH to be consumed in vivo, resulting in GSH content lower than that of the blank control group, while the selenium-rich Cordyceps militaris wine group increases GSH and SOD content in organism to the level of the blank control group and slightly higher. The selenium-rich cordyceps militaris wine can be used as a free radical scavenger, can preferentially participate in oxidation defense with GSH, and has better capability of protecting liver from being damaged. The results show that the selenium-rich cordyceps militaris wine has a certain protection effect on acute liver injury caused by alcohol.
TABLE 3 conventional index of blood for mice under different treatments
Note: different lower case letters indicate that the treatments have a significant difference P < 0.05. The same applies below.
TABLE 4 ALT, ALDH content in plasma of mice under different treatments
TABLE 5 contents of SOD, CAT, MDA, and GSH in the livers of mice treated differently
Although the invention has been described in detail hereinabove with respect to a general description and specific embodiments thereof, it will be apparent to those skilled in the art that modifications or improvements may be made thereto based on the invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (5)
1. A selenium-rich Cordyceps militaris wine with liver protecting function is characterized in that the selenium-rich Cordyceps militaris wine is prepared by taking selenium-rich Cordyceps militaris as raw material, preparing selenium-rich Cordyceps militaris extract by alcohol extraction, and blending with pure grain wine;
the selenium-rich cordyceps militaris contains 78-750 mu g/L of organic selenium, 1000mg/L of cordycepin, 1500mg/L of adenosine, 0.7-7.5mg/mL of cordycepic acid, 0.5-26.9mg/mL of beta-carotene and 15-68 degrees of alcohol.
2. The selenium-enriched cordyceps militaris wine of claim 1, wherein the extraction reagent used in the alcohol extraction method is pure grain wine.
3. The selenium-rich cordyceps militaris extract is characterized in that the preparation method of the selenium-rich cordyceps militaris extract comprises the following steps:
A. obtaining selenium-rich cordyceps militaris: culturing Cordyceps militaris CP1(Cordyceps militaris CP1) with a preservation number of CCTCC NO of M2019671 in a culture medium added with nano-selenium by adopting a cultivation method of selenium-rich Cordyceps militaris disclosed in CN201710433450.3 to obtain selenium-rich Cordyceps militaris with organic selenium content of 120-140 mg/kg;
B. preparation of selenium-rich Cordyceps militaris leaching liquor
B1, drying and crushing the selenium-rich cordyceps militaris, sieving the crushed selenium-rich cordyceps militaris with a sieve of 150 meshes and 200 meshes to obtain selenium-rich cordyceps militaris sporocarp powder, soaking the selenium-rich cordyceps militaris sporocarp powder in pure grain wine with the temperature of 15-68 ℃ according to the material-liquid ratio of 2.5-7.5%, uniformly stirring, carrying out 80-100HZ ultrasonic extraction for 15-45min, standing at the temperature of 15-20 ℃ for 9-12h, and filtering and clarifying to obtain a supernatant stock solution;
b2, adding 68-degree pure grain wine with the same volume as that of the 15-68-degree pure grain wine used in B1 into the residue after filtration of B1, stirring and uniformly mixing, carrying out ultrasonic extraction at 80-100HZ for 15-45min, standing for 9-12h at 15-20 ℃, filtering and clarifying to obtain a supernatant stock solution;
b3, repeating the step B23;
C. and (4) combining the supernatant stock solutions obtained in the step (B) to obtain the selenium-rich cordyceps militaris extract.
4. The application of the selenium-rich cordyceps militaris extract as claimed in claim 3 in the fields of food, health products and feed.
5. The method for preparing selenium-enriched cordyceps militaris wine as recited in claim 1 or 2, wherein the method comprises: blending the selenium-rich cordyceps militaris extract of claim 3 with pure grain wine in proportion to obtain the selenium-rich cordyceps militaris wine.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910920127.8A CN110591865A (en) | 2019-09-26 | 2019-09-26 | Selenium-rich cordyceps militaris wine with liver protection function and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201910920127.8A CN110591865A (en) | 2019-09-26 | 2019-09-26 | Selenium-rich cordyceps militaris wine with liver protection function and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN110591865A true CN110591865A (en) | 2019-12-20 |
Family
ID=68863787
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201910920127.8A Pending CN110591865A (en) | 2019-09-26 | 2019-09-26 | Selenium-rich cordyceps militaris wine with liver protection function and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN110591865A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023082217A1 (en) * | 2021-11-13 | 2023-05-19 | 广东暨创硒源纳米研究院有限公司 | Use of nano-selenium cordyceps militaris aqueous extract in reducing radiotherapy injury and protective agent |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102296014A (en) * | 2011-07-26 | 2011-12-28 | 湖北圣峰药业有限公司 | Preparation method for selenium cordyceps wine |
| WO2014055035A1 (en) * | 2012-10-04 | 2014-04-10 | Phyto Pharma Pte Ltd | New cordyceps militaris strain cbs 132098 and biologically active fruiting bodies, mycelial biomass and extracts therefrom |
| CN103740556A (en) * | 2013-12-20 | 2014-04-23 | 正源堂（天津）生物科技有限公司 | Preparation method of dry cordyceps militaris compound wine |
| CN105154297A (en) * | 2015-09-23 | 2015-12-16 | 安徽阳光药业有限公司 | New method for preparing health-preserving health-care cordyceps sinensis wine |
| CN106978300A (en) * | 2017-04-24 | 2017-07-25 | 广西环江仙草农舍生物科技有限公司 | The preparation method of cordyceps wine |
| CN107164237A (en) * | 2017-06-09 | 2017-09-15 | 中国农业大学 | Selenium-enriched cordceps militaris and its cultural method and application |
-
2019
- 2019-09-26 CN CN201910920127.8A patent/CN110591865A/en active Pending
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102296014A (en) * | 2011-07-26 | 2011-12-28 | 湖北圣峰药业有限公司 | Preparation method for selenium cordyceps wine |
| WO2014055035A1 (en) * | 2012-10-04 | 2014-04-10 | Phyto Pharma Pte Ltd | New cordyceps militaris strain cbs 132098 and biologically active fruiting bodies, mycelial biomass and extracts therefrom |
| CN103740556A (en) * | 2013-12-20 | 2014-04-23 | 正源堂（天津）生物科技有限公司 | Preparation method of dry cordyceps militaris compound wine |
| CN105154297A (en) * | 2015-09-23 | 2015-12-16 | 安徽阳光药业有限公司 | New method for preparing health-preserving health-care cordyceps sinensis wine |
| CN106978300A (en) * | 2017-04-24 | 2017-07-25 | 广西环江仙草农舍生物科技有限公司 | The preparation method of cordyceps wine |
| CN107164237A (en) * | 2017-06-09 | 2017-09-15 | 中国农业大学 | Selenium-enriched cordceps militaris and its cultural method and application |
Non-Patent Citations (1)
| Title |
|---|
| 赵志君等: "蛹虫草子实体保健酒的研制", 《酿酒》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2023082217A1 (en) * | 2021-11-13 | 2023-05-19 | 广东暨创硒源纳米研究院有限公司 | Use of nano-selenium cordyceps militaris aqueous extract in reducing radiotherapy injury and protective agent |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104997091B (en) | Cordyceps militaris instant solid beverage | |
| CN106244371B (en) | A kind of hypoglycemic rhizoma polygonati grape health care wine and its production technology | |
| CN101991043A (en) | Method for processing compound oat nutritious food with liquid fermentation tricholoma matsutake mycelium polysaccharide | |
| CN104694358B (en) | Cornu cervi healthcare wine for boosting immunity and preparation method thereof | |
| CN107418803A (en) | A kind of pear beer and its preparation technology | |
| KR20070105486A (en) | Tea and tea manufacturing method for improving liver function and curing of hangover | |
| CN107119096B (en) | Preparation method and application of pholiota nameko active peptide | |
| CN114306398A (en) | Application of phellinus igniarius fermented extract | |
| CN106174475A (en) | A kind of selenium protein holoside nutrient composite powder and preparation method thereof | |
| CN1687361A (en) | Tea trea flower wine and preparation method thereof | |
| KR101663379B1 (en) | MANUFACTURE OF FERMENTED Allium hookeri FROM YEASTS AND NATURAL ENZYME AND PREPARATION OF COMBINED BEVERAGE FOR QUENCHING THIRST | |
| CN108795684A (en) | One kind is enriched blood lipid-loweringing jujube health preserving vinegar and preparation method thereof | |
| CN110591865A (en) | Selenium-rich cordyceps militaris wine with liver protection function and preparation method thereof | |
| CN103497867B (en) | Mango banana fruit wine and production method thereof | |
| CN105733902B (en) | A kind of preparation method for the type Liu Yazi pueraria lobata yellow rice wine that relieves the effect of alcohol | |
| CN106811371A (en) | A kind of blackberry, blueberry health liquor and preparation method thereof | |
| CN106562164A (en) | Compound edible-fungus solid beverage | |
| CN1075067A (en) | The preparation method of north cordyceps sinensis beverage | |
| CN113907231B (en) | Preparation method of mulberry leaf biological beverage and product | |
| KR20170015618A (en) | Functional alcoholic liquors using cordyceps mushroomand method of producing the same | |
| CN105713787B (en) | A kind of preparation method of liver-protecting type safflower pueraria lobata yellow rice wine | |
| CN1091913A (en) | Bamboo shoot food | |
| CN102851164A (en) | Inonotus obliquus dry red wine | |
| CN102860497B (en) | Functional Pleurotus eryngii food and preparation method thereof | |
| CN1466990A (en) | Health food for protecting liver and adjusting blood fat and method for preparing the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20191220 |