CN1695728A - Oral taking preparation of Chinese traditional medicine for nourishing the kidney and the lung - Google Patents
Oral taking preparation of Chinese traditional medicine for nourishing the kidney and the lung Download PDFInfo
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- CN1695728A CN1695728A CN 200510003054 CN200510003054A CN1695728A CN 1695728 A CN1695728 A CN 1695728A CN 200510003054 CN200510003054 CN 200510003054 CN 200510003054 A CN200510003054 A CN 200510003054A CN 1695728 A CN1695728 A CN 1695728A
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- poria
- radix ophiopogonis
- schisandrae chinensis
- fructus schisandrae
- fructus corni
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Abstract
An orally-taken Chinese medicine in the form of capsule, dripping pill, tablet, particle, pill, or oral liquid for nourishing kidney and lung is prepared from 6 Chinese-medicinal materials including ophiopogon root, prepared rehmannia root, tree peony bark, yam, etc. Its preparing process is also disclosed.
Description
Technical field: the present invention relates to a kind of nourishing kidney and support oral preparation of Chinese traditional medicinal of lung and preparation method thereof, belong to the field of medicine technology.
Technical background: deficiency of YIN of both the lung and kidney, vertigo and tinnitus, diseases such as soreness of the waist and knees are the common diseases that threatens current people's health, have brought great misery to extensive patients.Commercially available MAIWEIDIHUANGPIAN, MAIWEIDIHUANGWAN and Maiwei rehmannia-root oral liquid have the effect that lung is supported in nourishing kidney, its prescription be on the basis of Liuwei Dihuang preparation, increase Radix Ophiopogonis and Fructus Schisandrae Chinensis totally eight flavor medicines form, just in the Radix Rehmanniae Preparata nourishing kidney yin, life essence-filling, marrow-benefitting, the Fructus Corni liver and kidney tonifying nourishes spleen the moon; The Rhizoma Dioscoreae nourishing lung and kidney nourishes spleen the moon; Cortex Moutan is let out the kidney fire clearly; Poria, Rhizoma Alismatis clearing away heat and promoting diuresis; All medicines are worked in coordination, and play the merit that nourishing the liver and kidney and foster lung promote the production of body fluid altogether.Be treatment deficiency of YIN of both the lung and kidney, hectic fever night sweat, dry pharynx hemoptysis, vertigo and tinnitus, soreness of the waist and knees and one of the better medicine of disease of quenching one's thirst.More representational in the method for producing Maiwei-dihuang preparation is to adopt the method for putting down in writing on the existing ministry standard, and wherein, MAIWEIDIHUANGPIAN is that Radix Ophiopogonis, Rhizoma Dioscoreae, Poria, Cortex Moutan are ground into fine powder; Fructus Schisandrae Chinensis, Radix Rehmanniae Preparata, Fructus Corni, Rhizoma Alismatis water decoct, and are condensed into thick paste, are mixed with fine powder again, and the back tabletting of granulating obtains medicine.Maiwei rehmannia-root oral liquid is that all medical material waters are decocted, concentrate, place and spend the night, the filtrate reconcentration, and cold preservation and makes medicine after sucrose mixes; MAIWEIDIHUANGWAN is that Rhizoma Alismatis, Radix Ophiopogonis, Poria are decocted, and is condensed into clear paste; Radix Rehmanniae Preparata decocts separately, is condensed into clear paste; The Cortex Moutan of Fructus Schisandrae Chinensis, recipe quantity 56% and 59% Fructus Corni, 70% ethanol percolate extraction reclaim ethanol and are condensed into clear paste; Residue Rhizoma Dioscoreae, Cortex Moutan and powder of Fructus Corni are broken into fine powder, and with above-mentioned each clear paste mixing, pill obtains medicine again.And the method for making of the MAIWEIDIHUANGWAN of 2005 editions records of Chinese Pharmacopoeia is that all pulverizing medicinal materials are become fine powder, sieves, and makes medicine with the general ball of refined honey behind the mixing.We find after deliberation, because above technology is perfect inadequately, so contain a large amount of invalid components and impurity in the Maiwei-dihuang preparation that makes in order to the top method, dose is big during use, brings psychological uneasiness to the patient, and bioavailability is not high; By a large amount of experiments, we find, in eight herbal medicines that constitute Maiwei-dihuang preparation, contain in the Cortex Moutan to contain in paeonol, the Fructus Corni and contain schisandrin B in ursolic acid, the Fructus Schisandrae Chinensis, contain Radix Ophiopogonis total saponins in Radix Ophiopogonis, because paeonol, ursolic acid, schisandrin B, Radix Ophiopogonis total saponins all are the compositions that have important effect in this medicine, in addition because paeonol is a volatile component, then impurity content is many if directly be used as medicine after pulverizing, heating decocting in water or volatilize easily again with the ethanol percolate extraction paeonol; And ursolic acid, schisandrin B, Radix Ophiopogonis total saponins all are insoluble in water, are soluble in alcohol, so simple water boiling method makes that above effective component extraction rate is low, if directly be used as medicine after pulverizing and make and contain a large amount of invalid elements in the medicine, and the effective component extraction rate of ethanol percolate extraction Fructus Schisandrae Chinensis and Fructus Corni is not high enough, so not ideal enough in order to the clinical efficacy of the Maiwei-dihuang preparation of top method preparation.
Summary of the invention: the objective of the invention is to: oral preparation of Chinese traditional medicinal of the foster lung of a kind of nourishing kidney and preparation method thereof is provided, and this preparation is meant all oral formulations clinically, comprising: capsule, drop pill, tablet, granule, pill, oral liquid etc.; Another object of the present invention is the preparation method of the Chinese medicine preparation of the foster lung of these nourishing kidneys of open preparation.In the present invention, have volatile characteristics at the paeonol in the Cortex Moutan, the present invention adopts steam distillation to extract paeonol, be insoluble in water, be soluble in the characteristics of alcohol at the ursolic acid in the Fructus Corni, schisandrin B and the Radix Ophiopogonis total saponins in Radix Ophiopogonis in the Fructus Schisandrae Chinensis, the present invention adopts the method for ethanol extraction to extract ursolic acid, schisandrin B and Radix Ophiopogonis total saponins.Because it is higher to have an effective component content with the Maiwei-dihuang preparation of the inventive method preparation, so can reach the effect that improves bioavailability.The present invention constitutes like this: calculate according to composition by weight: it mainly by Radix Ophiopogonis 30-120, Fructus Schisandrae (processed) 20-80, Radix Rehmanniae Preparata 80-320, Fructus Corni (processed) 40-160, Cortex Moutan 30-120, Rhizoma Dioscoreae 40-160, Poria 30-120 and Rhizoma Alismatis 30-120 and adjuvant be prepared from.Specifically: calculate according to composition by weight: it mainly is prepared into all oral formulations clinically by Radix Ophiopogonis 60, Fructus Schisandrae (processed) 40, Radix Rehmanniae Preparata 160, Fructus Corni (processed) 80, Cortex Moutan 60, Rhizoma Dioscoreae 80, Poria 60 and Rhizoma Alismatis 60 and adjuvant.Comprise: capsule, drop pill, tablet, granule, pill or oral liquid.
The preparation technology of preparation of the present invention is: Poria partly or entirely is ground into the above fine powder of 100 orders, Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni are concentrated into the thick paste shape with ethanol extraction, filtration, filtrate; With aqueous solution and the medicinal residues after the Cortex Moutan distillation, medicinal residues and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis behind Radix Ophiopogonis, Fructus Schisandrae Chinensis, the Fructus Corni ethanol extraction decoct with water, extracting solution filters, merging filtrate, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni thick paste again, Poria powder and paeonol mix homogeneously add different adjuvants again and can make different preparations according to the method for routine.
Concrete preparation method is: Poria partly or entirely is ground into the above fine powder of 100 orders, Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is condensed into extractum, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, be condensed into extractum, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni extractum, Poria powder and paeonol mix homogeneously add different adjuvants again and can make different preparations according to the method for routine.
But in experimenting, we find out that, and preparation of the present invention does not also reach best refining effect, if utilize the active ingredient in the macroporous adsorbent resin method extraction medical material in this preparation, this medicine is with refine more, and drug effect is more remarkable.So the preparation method of preparation of the present invention can also partly or entirely be ground into the above fine powder of 100 orders for: Poria; Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is condensed into extractum, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, pass through macroporous adsorptive resins, ethanol with 50-80% carries out eluting, reclaim ethanol, be condensed into extractum, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni extractum, Poria powder and paeonol mix homogeneously add different adjuvants again and can make different preparations according to the method for routine.
We find again in experimentation, also exist volatile shortcoming in order to contained paeonol in the Maiwei-dihuang preparation of top method preparation, if with cyclodextrin with the paeonol enclose, just can prevent that paeonol from volatilizing, and guarantees the curative effect of this medicine.So the preparation method of preparation of the present invention can also partly or entirely be ground into the above fine powder of 100 orders for: Poria; The Cortex Moutan steam distillation, distillate carries out enclose with the cyclodextrin that 0.5-5 doubly measures, and the clathrate cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is condensed into extractum, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, pass through macroporous adsorptive resins, ethanol with 50-80% carries out eluting, reclaim ethanol, be condensed into extractum, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni extractum, Poria powder and paeonol cyclodextrin clathrate mix homogeneously add different adjuvants again and can make different preparations according to the method for routine.
More specific says: the preparation technology of capsule of the present invention is: Poria partly or entirely is ground into the above fine powder of 100 orders; Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, by macroporous adsorptive resins, carry out eluting with the ethanol of 50-80%, reclaim ethanol, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, the Fructus Corni thick paste, Poria powder mixing granulation, microwave drying, pulverize, with the paeonol mix homogeneously, granulate again, incapsulate promptly.
The preparation technology of drop pill of the present invention is: Poria partly or entirely is ground into 80-100 purpose fine powder; Place it in micronizing equipment relaying continued powder again and be broken to 200-300 purpose micropowder; The Cortex Moutan steam distillation, distillate carries out enclose with the cyclodextrin that 0.5-5 doubly measures, and the clathrate cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is condensed into clear paste, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, by macroporous adsorptive resins, carry out eluting with the ethanol of 50-80%, reclaim ethanol, be condensed into clear paste, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni clear paste and Poria micropowder mixing granulation, drying is again with paeonol cyclodextrin clathrate mix homogeneously; The medicated powder of gained or granule are added in Macrogol 4000, polyethylene glycol 6000, sodium stearate or the glycerol gelatin matrix of heating and melting, keep 78-102 ℃ of steady temperature, splash in the methyl-silicone oil, collect drop pill promptly with dropper.
The preparation technology of pill of the present invention is: Poria partly or entirely is ground into the above fine powder of 100 orders; Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, by macroporous adsorptive resins, carry out eluting with the ethanol of 50-80%, reclaim ethanol, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni thick paste and Poria powder mix, and carry out spray drying, pulverize, cross the above screen cloth of 60 orders and 60 orders, add the paeonol mix homogeneously, add the refined honey that medicated powder amount 0.3-1 doubly measures again and close medicine, make honeyed pill and get final product.
The preparation technology of tablet of the present invention is: Poria partly or entirely is ground into the above fine powder of 100 orders; The Cortex Moutan steam distillation, distillate carries out enclose with the cyclodextrin that 0.5-5 doubly measures, and the clathrate cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, pass through macroporous adsorptive resins, ethanol with 50-80% carries out eluting, reclaim ethanol, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni thick paste and Poria powder mix, and carry out spray drying, add recipe quantity 5-20% starch and above-mentioned paeonol cyclodextrin clathrate mixing, use alcohol granulation, add dried grain amount 0.5-1% magnesium stearate mixing again, tabletting, with the 2-8% film coating agent plain sheet is carried out coating, promptly get tablet.
The preparation technology of granule of the present invention is: Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filtration, merging filtrate also are concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, the decocting that medicinal residues behind Radix Ophiopogonis, Fructus Schisandrae Chinensis, the Fructus Corni ethanol extraction and Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis adding 3-10 doubly measure boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni thick paste again, the auxiliary materials and mixing that paeonol and 2-5 doubly measure is granulated, and promptly gets granule.The employed adjuvant of granule of the present invention comprises dextrin, sucrose, sweeting agent, lactose.
The preparation technology of oral liquid of the present invention is: the Cortex Moutan steam distillation, collect distillate promptly; The decocting that remaining medicine adding 3-10 doubly measures boils 1-5 time, and each 1-5 hour, extracting solution filtered, merging filtrate filters, and filtrate concentrates, left standstill 10-50 hour, and got supernatant, filter, filtrate continuation concentrates, and cold preservation 10-50 hour, filters filtrate adding sucrose and makes final sugar content 5-60%, boil dissolving, filter, add the 0.06%-0.8% sodium benzoate, add water to full dose, stir evenly, promptly.
Cortex Moutan directly extracts the method for paeonol and is among the present invention: medical material adds 5-10 times of water gaging, adds the ethyl acetate of 1.0-2.5% in the improvement oil water separator, distillation extraction 8-14h, after extracting the volatile oil in the distillate, the reclaim under reduced pressure ethyl acetate, cold drying is ground into fine powder promptly.The method of said cyclodextrin of volatile oil enclose is a saturated water solution method; The enclose temperature is 30-40 ℃, and ultrasonic time is 25-35min.Cyclodextrin described in the present invention comprises alpha-cyclodextrin, beta-schardinger dextrin-, gamma-cyclodextrin, and the best is a beta-schardinger dextrin-.
Compared with prior art, the present invention adopts steam distillation to extract paeonol in the Cortex Moutan, has avoided conventional water boiling and extraction or ethanol percolate extraction to cause the loss of volatile oil, or it is too high directly to pulverize the impurity content that causes of being used as medicine; Use cyclodextrin inclusion compound volatile oil, can prevent the paeonol volatilization; Be insoluble in water, be soluble in the characteristics of alcohol at the ursolic acid in the Fructus Corni, schisandrin B and the Radix Ophiopogonis total saponins in Radix Ophiopogonis in the Fructus Schisandrae Chinensis in addition, adopt the method for ethanol extraction to extract ursolic acid, schisandrin B and Radix Ophiopogonis total saponins, when having avoided conventional water boiling and extraction or ethanol percolate extraction, the low or direct pulverizing of the above effective component extraction rate high shortcoming of invalid element content of being used as medicine; Adopt the macroporous adsorbent resin method to extract active ingredient in the medical material, make the refine more of this medicine, drug effect is more remarkable, for deficiency of YIN of both the lung and kidney, and hectic fever night sweat, the dry pharynx hemoptysis, vertigo and tinnitus, soreness of the waist and knees etc. have therapeutical effect preferably.Preparation and existing preparation with the inventive method preparation compare, and dose reduces, the bioavailability height; To have specific surface area big for the drop pill of the inventive method preparation and capsule in addition, and the characteristics that dissolution rate is fast have also been covered disagreeable tastes such as the hardship of existing preparation oral liquid, concentrated pill, puckery, acid, more can make things convenient for the patient to take.It is little that the granule of the inventive method preparation has fineness, and uniform particles be easy to the characteristics dissolving and absorb, and mouthfeel is good, and the no smell of burning has overcome the tart flavour that former preparation brings medicine, simultaneously, has got rid of the side effect that the insoluble composition of part is detained in the oral cavity.It is big that tablet of the present invention, pill and oral liquid overcome former preparation dose, absorbs bad shortcoming.And tablet mouldability, disintegrative are good, can reduce particulate hygroscopicity significantly, increase stability of drug products.In addition, needs of medical treatment are convenient in the dosage form variation, have reached the purpose of invention.
The present invention tests 1: Fructus Schisandrae Chinensis and Fructus Corni extraction process experimentation
Take by weighing Fructus Schisandrae Chinensis, each 50g of Fructus Corni respectively, with 10 times of amount alcohol reflux of 80% 2 times, each 2 hours, extracting solution reclaimed ethanol, promptly; Other gets Fructus Schisandrae Chinensis, each 50g of Fructus Corni, and the soak with ethanol with 70% is the percolation extraction after 24 hours, and extracting solution reclaims ethanol, promptly; Detect the wherein content of ursolic acid and schisandrin B respectively, calculate extraction ratio, the result is:
| Project | Ursolic acid | Tasteless sub-second element |
| Alcohol reflux | ??2.3％ | ??56.2％ |
| Ethanol percolate extraction | ??1.51％ | ??49.7％ |
Show that according to above experimental result alcohol reflux is better than ethanol percolate extraction.The present invention tests 2: preparation of the present invention is to the influence of yin deficiency syndrome animal pattern
One, material
1, medicine: the pill of the tablet of the present invention's preparation, the present invention's preparation, the capsule of the present invention's preparation all prepare according to the inventive method; MAIWEIDIHUANGPIAN, MAIWEIDIHUANGWAN, all commercially available; Trilute (T3).
2, animal Kunming mouse and SD are rat.
Two, method and result
1, to the influence of deficiency of YIN mice plasma CAMP and hepatic glycogen: test adopts the T3 method to set up the yin deficiency syndrome animal model.50 of male mices, body weight 18～22g divides 5 groups at random, gavages distilled water or medicine every day 1 time continuous 10 days, in administration beginning in the 6th day, gavages T318ul/ moulding in only continuous 5 days.Experiment finishes to put to death animal, measures plasma C AMP with the protein binding method, surveys hepatic glycogen with anthrone method, the results are shown in following table.
Tablet of the present invention is to the influence of deficiency of YIN mice plasma CAMP, hepatic glycogen (x ± SD)
| Group | Dosage (g/kg) | Number of animals (only) | Plasma C AMP (pM/ml) | Hepatic glycogen (g/100g liver) |
| Matched group | ??- | ??10 | ??92.74±27.05 | ??1.27±0.41 |
| The moulding group | ??- | ??10 | ??127.88±29.75^ | ??0.85±0.37^ |
| Tablet of the present invention | ??1.5 | ??10 | ??91.45±21.15 | ??1.30±0.40 |
| MAIWEIDIHUANGPIAN | ??3 | ??10 | ??91.21±12.78 | ??1.31±0.47 |
As can be seen from the above table, tablet 1.5g/kg dosage group of the present invention and commercially available MAIWEIDIHUANGPIAN 3g/kg dosage group compare there was no significant difference P＞0.05.
2, to the influence of deficiency of YIN rat body weight, lipid peroxide, adrenal cortex function and hemogram: rat male and female dual-purpose, body weight 226.4 ± 3.4g is divided into 5 groups at random, perfusion every day or with volume distilled water 1 time, continuous 10 days.Gavaged T on the 7th day in administration
3500ul/kg every day 1 time, for three days on end.Animal finish is put to death in experiment, gets liver, adrenal gland and blood sample specimen, detects liver MDA with the thiobarbituricacid colorimetry respectively, with 2, the ascorbic content of 4-dinitrophenylhydrazine determination of color adrenal gland, and observes the change of hemogram, the results are shown in following table
Pill of the present invention is to the influence of deficiency of YIN rat body weight, MDA and VitC (x ± SD)
| Group | Dosage (g/kg) | Body weight increase and decrease (g) (before and after the administration) | ??MDA ??(10 -7The mol/g liver) | VitC (mg/100g adrenal gland) |
| Matched group | ??- | ??8.0±12.3 | ??1.9±0.1 | ??295.1±21.3 |
| The moulding group | ??- | ??-13.5±4.1^^ | ??2.5±0.4^^ | ??229.4±83.4^ |
| Pill of the present invention | ??1 | ??-7.9±7.5 | ??2.0±0.3 | ??295.8±47.9 |
| MAIWEIDIHUANGWAN | ??2 | ??-8.0±3.2 | ??2.1±0.6 | ??296.0±41.3 |
As can be seen from the above table, pill 1g/kg dosage group of the present invention and commercially available MAIWEIDIHUANGWAN 2g/kg dosage group compare there was no significant difference P＞0.05.
Capsule of the present invention is to the influence of deficiency of YIN rat hemogram (x ± SD)
| Group | Dosage (g/kg) | ??Hb ??(g/L) | ??RBC ??(1012/L) | ??WBC ??(10 8/L) | WBC classify (%) | |
| Neutral leaflet nuclear | Lymphocyte | |||||
| Matched group | ??- | ??150.0±15.9 | ??6.5±1.1 | ??11.0±2.0 | ??29±14 | ??67±14 |
| The moulding group | ??- | ??142.6±13.3 | ??6.7±0.6 | ??10.9±2.5 | ??43±5^ | ??55±4^ |
| Pill of the present invention | ??1 | ??149.8±14.5 | ??6.4±1.1 | ??10.7±3.6 | ??38±9 | ??64±10 |
| MAIWEIDIHUANGWAN | ??2 | ??148.6±19.2 | ??6.4±2.0 | ??10.9±3.0 | ??39±11 | ??63±11 |
As can be seen from the above table, pill 1g/kg dosage group of the present invention and commercially available MAIWEIDIHUANGWAN 2g/kg dosage group compare there was no significant difference P＞0.05.
The above results shows, pill 1g/kg dosage group of the present invention and commercially available MAIWEIDIHUANGWAN 2g/kg dosage group therapeutic equivalence.
3, to deficiency of YIN effect of immunologic function
3.1 hemolytic plaque test: get body weight 17～22g mice male and female dual-purpose, grouping administration and use T
3Method is set up the yin deficiency syndrome animal model all with 2.1.In administration in the time of the 6th day, with fresh sheep red blood cell (SRBC) suspension (SR-BC) immunity, every Mus 4 * 10
8RBC/0.5ml ip.After immunity, put to death animal on the 4th day, separation of serum and preparation splenocyte suspension, the plaque number with on every slide of agarose slide method counting calculates cell volume.According to splenocyte concentration correction in cell volume and the mixed liquor per 1,000,000 (1 * 10
6) to contain plaque forming cells's number in the splenocyte (be PFC number/10
6Splenocyte), the results are shown in following table; The blood sample specimen is pressed literature method and is detected the serum agglutinin, and the result represents that with the log2 titre result also sees the following form; Other has experiment to get 60 of mices, body weight 17～22g, with method grouping perfusion, with the moulding of T3 method and with fresh SRBC immunity, test and finish to put to death animal, take blood preparation, the reference literature method is done the E bow structure and is formed experiment, observation E bow structure formation rate, promptly the erythrocyte Flos Rosae Rugosae forms cell (ERFC) formation rate, the results are shown in following table.
Capsule of the present invention forms the influence (x ± SD) of cell to deficiency of YIN mouse antibodies
| Group | Dosage (g/kg) | Hemolysis plaque (PFC number/10 6Splenocyte) | Serum agglutinin (log2 titre) | ??ERFC ??(％) |
| Matched group | ??- | ??646.6±298.1 | ??4.6±0.7 | ??14.3±5.1 |
| The moulding group | ??- | ??354.7±105.8 | ??3.3±0.5 | ??11.4±5.3 |
| Capsule of the present invention | ??1 | ??1043.9±164.8 | ??3.8±0.6 | ??18.3±7.4 |
| MAIWEIDIHUANGWAN | ??2 | ??1044.9±448.9 | ??3.7±0.9 | ??17.0±4.7 |
The above results shows: each administration group all can make deficiency of YIN mice plaque forming cells number obviously raise; Make deficiency of YIN mice serum agglutinin return to normal level.Increase deficiency of YIN mice E bow structure formation rate.So significant difference (P＜0.05) is more all arranged for capsule group of the present invention in addition and MAIWEIDIHUANGWAN group capsule 1g/kg dosage group of the present invention and commercially available MAIWEIDIHUANGWAN 2g/kg dosage group therapeutic equivalence.
The present invention tests 3: preparation of the present invention is to the intervention effect of interstitial pulmonary fibrosis rat cell factor network
One, materials and methods
1, animal and grouping: 48 of cleaning level SD male rats, body weight 180～210g.Completely random is divided into 4 groups, 12 every group, is respectively: the I group: the blank group, the II group: model group, the III group: drop pill group of the present invention prepares according to the inventive method.The IV group: MAIWEIDIHUANGWAN (concentrated pill), commercially available.
2, medicine: Bleocin Hydrochloride.
3, animal is handled: adopt the bleomycin intratracheal injection.Each group is anaesthetized through intraperitoneal injection with 1% pentobarbital sodium (0.4ml/100g body weight), the rat dorsal position is fixed, the descending cervical region median incision in the sterile working, successively separate soft tissue, expose trachea, wear long to stab and slowly inject bleomycin 5mg/kg body weight in the trachea, rotate animal after the injection immediately, sew up the incision.Matched group is with injecting the equivalent normal saline in the method bronchus.It is 6% solution that each treatment group medicine is made into concentration with physiological saline solution.On the same day after modeling, press the dosage of 1ml/100g and irritate stomach, 1 time/d for Manufactured pulmonary fibrosis animal.Matched group and model group are with method intragastric infusion equivalent normal saline.Respectively organized in the 7th, 28 day behind the Yu Zaomo 6 by preceding method anesthesia, fixing, cut through extracting arterial blood not.
4, histopathologic slide's preparation: put to death animal after the common carotid artery blood-letting, take out in the lungs input neutral formalin liquid of right side and fix, at the outer row sagittate section, embedding, section (5um).Row HE dyeing.
5, detect: adopt to put and exempt from method mensuration blood plasma TXB
2, ET-1, ET-1.Adopt the double-antibody sandwich euzymelinked immunosorbent assay (ELISA) to measure serum MCP-1.Each is organized data application SPSS11.0 and carries out variance analysis, and compares in twos, and P＜0.05 is for there being significant difference.
Two, result:
1, blood plasma TXB
2
Each experimental group rat plasma TXB
2Content (pg/ml, x ± s)
| Group | Dosage (g/kg) | ??n | ??7d | ??28d |
| The blank group | ??- | ??6 | ??107.622±16.471 | ??109.100±16.905 |
| Model group | ??- | ??6 | ??233.734±19.256 | ??250.794±23.108 |
| Drop pill group of the present invention | ??1g/kg | ??6 | ??116.729±20.094 | ??159.319±44.574 |
| The MAIWEIDIHUANGWAN group | ??3g/kg | ??6 | ??166.448±40.189 | ??226.607±36.105 |
2, blood plasma ET-1
Each experimental group rat plasma ET-1 content (pg/ml, x ± s)
| Group | Dosage (g/kg) | ??n | ??7d | ??28d |
| The blank group | ??- | ??6 | ??51.523±10.276 | ??51.515±10.445 |
| Model group | ??- | ??6 | ??75.987±10.128 | ??102.101±17.840 |
| Drop pill group of the present invention | ??1g/kg | ??6 | ??50.541±12.802 | ??50.311±4.499 |
| The MAIWEIDIHUANGWAN group | ??3g/kg | ??6 | ??65.990±16.473 | ??67.912±11.842 |
3, serum MCP-1
Each experimental group rat blood serum MCP-1 content (pg/ml, x ± s)
| Group | Dosage (g/kg) | ??n | ??7d | ??28d |
| The blank group | ??- | ??6 | ??10.895±1.748 | ??10.484±1.528 |
| Model group | ??- | ??6 | ??24.046±5.55 | ??43.772±22.378 |
| Drop pill group of the present invention | ??1g/kg | ??6 | ??11.959±6.729 | ??15.188±1.931 |
| The MAIWEIDIHUANGWAN group | ??3g/kg | ??6 | ??20.218±8.308 | ??28.692±3.887 |
According to above experimental result, owing between the treatment group significant difference (P＜0.05) is arranged, so drop pill group of the present invention and commercially available MAIWEIDIHUANGWAN (concentrated pill) group are relatively, under the identical situation of clinical consumption, drop pill group of the present invention contrasts the better efficacy of commercially available MAIWEIDIHUANGWAN, and bioavailability is higher.
The specific embodiment:
The embodiment of the invention 1: take by weighing: Radix Ophiopogonis 30g, Fructus Schisandrae Chinensis (system) 80g, Radix Rehmanniae Preparata 80g, Fructus Corni (system) 160g, Cortex Moutan 30g, Rhizoma Dioscoreae 40g, Poria 120g, Rhizoma Alismatis 30g, Oleum Glycines 75ml, tween 80 40ml, ethyl cellulose 50g; Preparation technology is: Poria powder is broken into 150 purpose fine powders, and Cortex Moutan adds 300ml water, adds 2.5% ethyl acetate in the improvement oil water separator, distillation extraction 14h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, cold drying is ground into fine powder and promptly gets Cortex Moutan extract; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni 4050ml 80% alcohol reflux 5 times, be respectively: 5h, 1h, 0.5h, 0.5h, 0.5h, filter, merging filtrate also is condensed into extractum, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis adds the 1500ml decocting and boils 5 times, be respectively: 5h, 3h, 1h, 1h, 1h, extracting solution filters, merging filtrate is condensed into extractum, adds above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni extractum, the Poria powder mix homogeneously, drying is ground into 100 purpose fine powders, adds the paeonol mix homogeneously, other gets Oleum Glycines, tween 80, the ethyl cellulose mixing, above-mentioned medicated powder is joined Oleum Glycines, mix homogeneously in the mixed solution of tween 80 and ethyl cellulose, pill promptly gets soft capsule.
The embodiment of the invention 2: take by weighing: Radix Ophiopogonis 60g, Fructus Schisandrae Chinensis (system) 40g, Radix Rehmanniae Preparata 160g, Fructus Corni (system) 80g, Cortex Moutan 60g, Rhizoma Dioscoreae 80g, Poria 60g, Rhizoma Alismatis 60g, micropowder cellulose 45g, kieselguhr 20g; Preparation technology is: Poria 30g is ground into 100 purpose fine powders; Cortex Moutan adds 300ml water, adds 1.0% ethyl acetate in the improvement oil water separator, distillation extraction 8h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni is used 1440ml 60% alcohol reflux 2 times respectively, each 2 hours, filter, merging filtrate also is condensed into extractum, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis adds the 990ml decocting boils 3 times, is respectively 3h, 2h, 1h, extracting solution filters, merging filtrate, by macroporous adsorptive resins, the ethanol with 50% carries out eluting, reclaims ethanol, be condensed into extractum, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni extractum, Poria powder mix homogeneously, drying, be ground into 100 purpose fine powders, add the paeonol mix homogeneously; The thing powder 200g that gets it filled mixes with micropowder cellulose 45g, kieselguhr 20g; put in the coating granulator; with 5% ethylmethylcellulose alcoholic solution is that binding agent is at first made 40 purpose master batches; put into the rotating disk of coating granulator after the oven dry again; be binding agent with 8% sodium carboxymethyl cellulose solution again, add mixed medicated powder rolling and make the micropill that particle diameter is 2mm, drying; incapsulate, promptly get slow releasing capsule.Technological parameter is: spouting velocity: 15r/min, engine speed: 80r/min is for powder speed: 15r/min.
The embodiment of the invention 3: take by weighing: Radix Ophiopogonis 120g, Fructus Schisandrae Chinensis (system) 20g, Radix Rehmanniae Preparata 320g, Fructus Corni (system) 40g, Cortex Moutan 120g, Rhizoma Dioscoreae 160g, Poria 30g, Rhizoma Alismatis 120g, beta-schardinger dextrin-40g, PVPP 57g, CMC-Ca 108g, micropowder silica gel 3g, MCC 200g, magnesium stearate 5g; Preparation technology is: Poria 6g is ground into 120 purpose fine powders; Cortex Moutan adds 960ml water, extracts with steam distillation, and distillate carries out enclose with beta-schardinger dextrin-, and inclusion method is a saturated water solution method, and the enclose temperature is 35-40 ℃, and ultrasonic time is 30min; The clathrate cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni 1800ml 70% alcohol reflux 3 times, each 1 hour, filter, merging filtrate also is condensed into extractum, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis adds the 5000ml decocting and boils 3 times, be respectively: 3h, 2h, 1h, extracting solution filters, and merging filtrate passes through macroporous adsorptive resins, ethanol with 80% carries out eluting, reclaim ethanol, be condensed into extractum, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni extractum, Poria powder, the paeonol cyclodextrin clathrate, PVPP 30g, CMC-Ca 60g mix homogeneously, dry, pulverize, cross 100 mesh sieves, standby; Other gets residue PVPP27g, CMC-Ca48g and micropowder silica gel, MCC, magnesium stearate respectively at below 40 ℃ dry 6 hours, pulverizes, and mistake 100 mesh sieves, standby; Get remaining PVPP, CMC-Ca, MCC, micropowder silica gel and above-mentioned medicated powder mix homogeneously are adhesive system soft material with HPMC, cross 30 mesh sieve preparations, and 60 ℃ of dryings added the magnesium stearate mix homogeneously after 2 hours, and tabletting promptly gets dispersible tablet.
The embodiment of the invention 4: take by weighing: Radix Ophiopogonis 60g, Fructus Schisandrae Chinensis (system) 40g, Radix Rehmanniae Preparata 160g, Fructus Corni (system) 80g, Cortex Moutan 60g, Rhizoma Dioscoreae 80g, Poria 60g, Rhizoma Alismatis 60g, beta-schardinger dextrin-20g, Macrogol 4000 or polyethylene glycol 6000 300ml; Preparation technology is: get Poria powder and be broken into 80 purpose fine powders; Place it in micronizing equipment relaying continued powder again and be broken to 200 purpose micropowders; Cortex Moutan water steam distillation and extraction, method is: add 480ml water, add 2.0% ethyl acetate in the improvement oil water separator, distillation extraction 10h, after extracting the volatile oil in the distillate, the reclaim under reduced pressure ethyl acetate, distillate carries out enclose with beta-schardinger dextrin-, the enclose temperature is 35 ℃, and ultrasonic time is 30min.The clathrate cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni adds 1080ml 95% alcohol reflux 2 times, each 1 hour, filter, merging filtrate also is condensed into clear paste, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis adds the 2400ml decocting and boils 3 times, decocting time is respectively 3h, 2h, 1h, extracting solution filters, merging filtrate, pass through macroporous adsorptive resins, ethanol with 70% carries out eluting, reclaim ethanol, be condensed into clear paste, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni clear paste and Poria micropowder mixing granulation, drying is again with paeonol cyclodextrin clathrate mix homogeneously, the medicated powder of gained or granule are added in the Macrogol 4000 or polyethylene glycol 6000 of heating and melting, keep 90-102 ℃ of steady temperature, splash in the methyl-silicone oil, promptly get dropping pill formulation with dropper.
The embodiment of the invention 5: take by weighing: Radix Ophiopogonis 60g, Fructus Schisandrae Chinensis (system) 40g, Radix Rehmanniae Preparata 160g, Fructus Corni (system) 80g, Cortex Moutan 60g, Rhizoma Dioscoreae 80g, Poria 60g, Rhizoma Alismatis 60g, alpha-cyclodextrin 20g, sodium stearate or glycerin gelatine 300ml; Preparation technology is: get Poria powder and be broken into 100 purpose fine powders; Place it in micronizing equipment relaying continued powder again and be broken to 300 purpose micropowders; Cortex Moutan water steam distillation and extraction, method is: add 480ml water, add 2.0% ethyl acetate in the improvement oil water separator, distillation extraction 10h, after extracting the volatile oil in the distillate, the reclaim under reduced pressure ethyl acetate, distillate carries out enclose with alpha-cyclodextrin, the enclose temperature is 35 ℃, and ultrasonic time is 30min.The clathrate cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni adds 1080ml 95% alcohol reflux 2 times, each 1 hour, filter, merging filtrate also is condensed into clear paste, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis adds the 2400ml decocting and boils 3 times, decocting time is respectively 3h, 2h, 1h, extracting solution filters, merging filtrate, pass through macroporous adsorptive resins, ethanol with 70% carries out eluting, reclaim ethanol, be condensed into clear paste, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni clear paste and Poria micropowder mixing granulation, drying is again with paeonol cyclodextrin clathrate mix homogeneously, the medicated powder of gained or granule are added in the sodium stearate or glycerol gelatin matrix of heating and melting, keep 78-90 ℃ of steady temperature, splash in the methyl-silicone oil, promptly get dropping pill formulation with dropper.
The embodiment of the invention 6: take by weighing: Radix Ophiopogonis 60g, Fructus Schisandrae Chinensis (system) 40g, Radix Rehmanniae Preparata 160g, Fructus Corni (system) 80g, Cortex Moutan 60g, Rhizoma Dioscoreae 80g, Poria 60g, Rhizoma Alismatis 60g; Preparation technology is: Poria 30g is ground into 120 purpose fine powders; Cortex Moutan water steam distillation and extraction paeonol, method are that Cortex Moutan adds 480ml water, add 2.0% ethyl acetate in the improvement oil water separator, distillation extraction 10h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 3 times, extraction time is respectively 1h, 0.5h, 0.5h, filter, merging filtrate also is concentrated into the thick paste shape, with the aqueous solution after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis adds the 3300ml decocting and boiled 5 hours, extracting solution filters, pass through macroporous adsorptive resins, ethanol with 50% carries out eluting, reclaim ethanol, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, the Fructus Corni thick paste, the Poria powder mixing granulation, microwave drying, pulverize, incapsulate, promptly get capsule preparations.
The embodiment of the invention 7: take by weighing: Radix Ophiopogonis 120g, Fructus Schisandrae Chinensis (system) 20g, Radix Rehmanniae Preparata 320g, Fructus Corni (system) 40g, Cortex Moutan 120g, Rhizoma Dioscoreae 160g, Poria 30g, Rhizoma Alismatis 120g, dextrin or sucrose 2000g; Preparation technology is: Poria powder is broken into 100 purpose fine powders, the Cortex Moutan steam distillation, method is: Cortex Moutan adds 720ml water, add 2.0% ethyl acetate in the improvement oil water separator, distillation extraction 10h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni are concentrated into the thick paste shape with ethanol extraction, filtration, filtrate; With aqueous solution and the medicinal residues after the Cortex Moutan distillation, medicinal residues and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis behind Radix Ophiopogonis, Fructus Schisandrae Chinensis, the Fructus Corni ethanol extraction decoct with water, extracting solution filters, merging filtrate, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni thick paste again, paeonol and dextrin or sucrose mix homogeneously, granulate, promptly get granular preparation.
The embodiment of the invention 8: take by weighing: Radix Ophiopogonis 30g, Fructus Schisandrae Chinensis (system) 80g, Radix Rehmanniae Preparata 80g, Fructus Corni (system) 160g, Cortex Moutan 30g, Rhizoma Dioscoreae 40g, Poria 120g, Rhizoma Alismatis 30g, sweeting agent or lactose 320g; Preparation technology is: the Cortex Moutan steam distillation, and method is: Cortex Moutan adds 240ml water, adds 1.5% ethyl acetate in the improvement oil water separator, distillation extraction 10h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 4 times, each 0.5 hour, filtration, merging filtrate also are concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, medicinal residues behind Radix Ophiopogonis, Fructus Schisandrae Chinensis, the Fructus Corni ethanol extraction and Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis add the 1620ml decocting and boil 3 times, decoct 3h, 1h, 1h respectively, extracting solution filters, merging filtrate, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni thick paste again, paeonol and sweeting agent or lactose mixing are granulated, and promptly get granule.
The embodiment of the invention 9: take by weighing: Radix Ophiopogonis 120g, Fructus Schisandrae Chinensis (system) 20g, Radix Rehmanniae Preparata 320g, Fructus Corni (system) 40g, Cortex Moutan 120g, Rhizoma Dioscoreae 160g, Poria 30g, Rhizoma Alismatis 120g, refined honey 250g; Preparation technology is: Poria 9g is ground into the above fine powder of 100 orders; The Cortex Moutan steam distillation, method is: Cortex Moutan adds 1080ml water, adds 2.5% ethyl acetate in the improvement oil water separator, distillation extraction 8h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, cold drying is ground into fine powder promptly.Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni is used 2700ml 90% alcohol reflux 5 hours, filter, merging filtrate also is concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the Rhizoma Alismatis water decocts, for the first time adding the 6210ml decocting boiled 3 hours, second to five time respectively the 4968ml decocting boiled 1 hour, extracting solution filters, merging filtrate, by macroporous adsorptive resins, the ethanol with 80% carries out eluting, reclaims ethanol, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni thick paste and Poria powder mix, and carry out spray drying, pulverize, cross 60 purpose screen clothes, add the paeonol mix homogeneously, add refined honey again and close medicine, make honeyed pill and promptly get pill.
The embodiment of the invention 10: take by weighing: Radix Ophiopogonis 120g, Fructus Schisandrae Chinensis (system) 20g, Radix Rehmanniae Preparata 320g, Fructus Corni (system) 40g, Cortex Moutan 120g, Rhizoma Dioscoreae 160g, Poria 30g, Rhizoma Alismatis 120g, refined honey 75g; Preparation technology is: Poria 9g is ground into the above fine powder of 100 orders; The Cortex Moutan steam distillation, method is: Cortex Moutan adds 720ml water, adds 1.0% ethyl acetate in the improvement oil water separator, distillation extraction 12h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, cold drying is ground into fine powder promptly.Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni is used 2700ml 90% alcohol reflux 5 hours, filter, merging filtrate also is concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the Rhizoma Alismatis water decocts, for the first time adding the 6210ml decocting boiled 3 hours, second to five time respectively the 4968ml decocting boiled 1 hour, extracting solution filters, merging filtrate, by macroporous adsorptive resins, the ethanol with 80% carries out eluting, reclaims ethanol, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni thick paste and Poria powder mix, and carry out spray drying, pulverize, cross 60 purpose screen clothes, add the paeonol mix homogeneously, add refined honey again and close medicine, make honeyed pill and promptly get pill.
The embodiment of the invention 11: take by weighing: Radix Ophiopogonis 30g, Fructus Schisandrae Chinensis (system) 80g, Radix Rehmanniae Preparata 80g, Fructus Corni (system) 160g, Cortex Moutan 30g, Rhizoma Dioscoreae 40g, Poria 120g, Rhizoma Alismatis 30g, gamma-cyclodextrin 5g, starch 28.5g, magnesium stearate 0.73g; Preparation technology is: Poria 96g is ground into 150 purpose fine powders; Cortex Moutan steam distillation, distillation extraction method be, medical material adds 300ml water, adds 2.5% ethyl acetate in the improvement oil water separator, distillation extraction 8h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, promptly.Distillate carries out enclose with gamma-cyclodextrin, and the enclose temperature is 35-40 ℃, and ultrasonic time is 30-35min.The clathrate cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 3 times, each 1 hour, filter, merging filtrate also is concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis adds the 522ml decocting respectively and boils 2 times, each 2 hours, extracting solution filters, merging filtrate, by macroporous adsorptive resins, the ethanol with 60% carries out eluting, reclaim ethanol, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni thick paste and Poria powder mix, and carry out spray drying, add starch and above-mentioned paeonol cyclodextrin clathrate mixing, use alcohol granulation, add the magnesium stearate mixing again, tabletting, with 2% film coating agent plain sheet is carried out coating, promptly get tablet.
The embodiment of the invention 12: take by weighing: Radix Ophiopogonis 30g, Fructus Schisandrae Chinensis (system) 80g, Radix Rehmanniae Preparata 80g, Fructus Corni (system) 160g, Cortex Moutan 30g, Rhizoma Dioscoreae 40g, Poria 120g, Rhizoma Alismatis 30g, gamma-cyclodextrin 30g, starch 114g, magnesium stearate 1.73g; Preparation technology is: Poria 96g is ground into 150 purpose fine powders; Cortex Moutan steam distillation, distillation extraction method be, medical material adds 300ml water, adds 2.5% ethyl acetate in the improvement oil water separator, distillation extraction 8h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, promptly.The distillate gamma-cyclodextrin carries out enclose, and the enclose temperature is 35-40 ℃, and ultrasonic time is 30-35min.The clathrate cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 3 times, each 1 hour, filter, merging filtrate also is concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis adds the 522ml decocting respectively and boils 2 times, each 2 hours, extracting solution filters, merging filtrate, by macroporous adsorptive resins, the ethanol with 70% carries out eluting, reclaim ethanol, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni thick paste and Poria powder mix, and carry out spray drying, add starch and above-mentioned paeonol cyclodextrin clathrate mixing, use alcohol granulation, add the magnesium stearate mixing again, tabletting, with 8% film coating agent plain sheet is carried out coating, promptly get tablet.
The embodiment of the invention 13: take by weighing: Radix Ophiopogonis 45g, Fructus Schisandrae Chinensis 30g, Radix Rehmanniae Preparata 120g, Fructus Corni (system) 60g, Cortex Moutan 45g, Rhizoma Dioscoreae 60g, Poria 45g, Rhizoma Alismatis 45g, sucrose 50g, sodium benzoate 0.6g; Cortex Moutan steam distillation, distillation extraction method be, medical material adds 480ml water, adds 2.0% ethyl acetate in the improvement oil water separator, distillation extraction 10h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, promptly; Remaining medicine adds the 5400ml decocting for the first time and boiled 4 hours, adds the 4320ml decocting for the second time and boils 2 hours, adds the 3240ml decocting for the third time and boils 1 hour, and extracting solution filters, merging filtrate filters, and filtrate concentrates, left standstill 12 hours, and got supernatant, filter, filtrate continuation concentrates, and cold preservation 24 hours filters filtrate and adds sucrose, boil dissolving, filter, add sodium benzoate, add water to full dose, stir evenly, promptly get oral liquid.
The embodiment of the invention 14: take by weighing: Radix Ophiopogonis 45g, Fructus Schisandrae Chinensis 30g, Radix Rehmanniae Preparata 120g, Fructus Corni (system) 60g, Cortex Moutan 45g, Rhizoma Dioscoreae 60g, Poria 45g, Rhizoma Alismatis 45g, sucrose 600g, sodium benzoate 8g; Cortex Moutan steam distillation, distillation extraction method be, medical material adds 600ml water, adds 2.0% ethyl acetate in the improvement oil water separator, distillation extraction 10h, extract the volatile oil in the distillate after, the reclaim under reduced pressure ethyl acetate, promptly; Remaining medicine adds the 5400ml decocting for the first time and boiled 4 hours, adds the 4320ml decocting for the second time and boils 2 hours, adds the 3240ml decocting for the third time and boils 1 hour, and extracting solution filters, merging filtrate filters, and filtrate concentrates, left standstill 12 hours, and got supernatant, filter, filtrate continuation concentrates, and cold preservation 24 hours filters filtrate and adds sucrose, boil dissolving, filter, add sodium benzoate, add water to full dose, stir evenly, promptly get oral liquid.
Claims (15)
1, the oral preparation of Chinese traditional medicinal of lung is supported in a kind of nourishing kidney, it is characterized in that: calculate according to composition by weight: it mainly by Radix Ophiopogonis 30-120, Fructus Schisandrae (processed) 20-80, Radix Rehmanniae Preparata 80-320, Fructus Corni (processed) 40-160, Cortex Moutan 30-120, Rhizoma Dioscoreae 40-160, Poria 30-120 and Rhizoma Alismatis 30-120 and adjuvant be prepared from.
2, support the oral preparation of Chinese traditional medicinal of lung according to the described nourishing kidney of claim 1, it is characterized in that: calculate according to composition by weight: it mainly is prepared into all oral formulations clinically by Radix Ophiopogonis 60, Fructus Schisandrae (processed) 40, Radix Rehmanniae Preparata 160, Fructus Corni (processed) 80, Cortex Moutan 60, Rhizoma Dioscoreae 80, Poria 60 and Rhizoma Alismatis 60 and adjuvant.
3, support the oral preparation of Chinese traditional medicinal of lung according to claim 1 or 2 described nourishing kidneys, it is characterized in that: said oral formulations comprises: capsule, drop pill, tablet, granule, pill or oral liquid.
4, support the preparation method of the oral preparation of Chinese traditional medicinal of lung as any described nourishing kidney among the claim 1-3, it is characterized in that: Poria partly or entirely is ground into the above fine powder of 100 orders, Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni are concentrated into the thick paste shape with ethanol extraction, filtration, filtrate; With aqueous solution and the medicinal residues after the Cortex Moutan distillation, medicinal residues and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis behind Radix Ophiopogonis, Fructus Schisandrae Chinensis, the Fructus Corni ethanol extraction decoct with water, extracting solution filters, merging filtrate, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni thick paste again, Poria powder and paeonol mix homogeneously add different adjuvants again and can make different preparations according to the method for routine.
5, support the preparation method of the oral preparation of Chinese traditional medicinal of lung according to the described nourishing kidney of claim 4, it is characterized in that: Poria partly or entirely is ground into the above fine powder of 100 orders, Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is condensed into extractum, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, be condensed into extractum, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni extractum, Poria powder and paeonol mix homogeneously add different adjuvants again and can make different preparations according to the method for routine.
6, support the preparation method of the oral preparation of Chinese traditional medicinal of lung according to claim 4 or 5 described nourishing kidneys, it is characterized in that: Poria partly or entirely is ground into the above fine powder of 100 orders; Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is condensed into extractum, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, pass through macroporous adsorptive resins, ethanol with 50-80% carries out eluting, reclaim ethanol, be condensed into extractum, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni extractum, Poria powder and paeonol mix homogeneously add different adjuvants again and can make different preparations according to the method for routine.
7, support the preparation method of the oral preparation of Chinese traditional medicinal of lung according to claim 4 or 5 described nourishing kidneys, it is characterized in that: Poria partly or entirely is ground into the above fine powder of 100 orders; The Cortex Moutan steam distillation, distillate carries out enclose with the cyclodextrin that 0.5-5 doubly measures, and the clathrate cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is condensed into extractum, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, pass through macroporous adsorptive resins, ethanol with 50-80% carries out eluting, reclaim ethanol, be condensed into extractum, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni extractum, Poria powder and paeonol cyclodextrin clathrate mix homogeneously add different adjuvants again and can make different preparations according to the method for routine.
8, support the preparation method of the oral preparation of Chinese traditional medicinal of lung according to the described nourishing kidney of claim 7, it is characterized in that: cyclodextrin described in the present invention comprises alpha-cyclodextrin, beta-schardinger dextrin-, gamma-cyclodextrin, and the best is a beta-schardinger dextrin-.
9, support the preparation method of the oral preparation of Chinese traditional medicinal of lung according to claim 4 or 5 described nourishing kidneys, it is characterized in that: the method that Cortex Moutan directly extracts paeonol is: medical material adds 5-10 times of water gaging, the ethyl acetate that adds 1.0-2.5% in the improvement oil water separator, distillation extraction 8-14h, after extracting the volatile oil in the distillate, the reclaim under reduced pressure ethyl acetate, cold drying is ground into fine powder promptly; The method of said cyclodextrin of volatile oil enclose is a saturated water solution method; The enclose temperature is 30-40 ℃, and ultrasonic time is 25-35min.
10, support the preparation method of the oral preparation of Chinese traditional medicinal of lung according to claim 4 or 5 described nourishing kidneys, it is characterized in that: the preparation technology of described capsule is: Poria partly or entirely is ground into the above fine powder of 100 orders; Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, by macroporous adsorptive resins, carry out eluting with the ethanol of 50-80%, reclaim ethanol, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, the Fructus Corni thick paste, Poria powder mixing granulation, microwave drying, pulverize, with the paeonol mix homogeneously, granulate again, incapsulate promptly.
11, support the preparation method of the oral preparation of Chinese traditional medicinal of lung according to claim 4 or 5 described nourishing kidneys, it is characterized in that: the preparation technology of described drop pill is: Poria partly or entirely is ground into 80-100 purpose fine powder; Place it in micronizing equipment relaying continued powder again and be broken to 200-300 purpose micropowder; The Cortex Moutan steam distillation, distillate carries out enclose with the cyclodextrin that 0.5-5 doubly measures, and the clathrate cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is condensed into clear paste, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, by macroporous adsorptive resins, carry out eluting with the ethanol of 50-80%, reclaim ethanol, be condensed into clear paste, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni clear paste and Poria micropowder mixing granulation, drying is again with paeonol cyclodextrin clathrate mix homogeneously; The medicated powder of gained or granule are added in Macrogol 4000, polyethylene glycol 6000, sodium stearate or the glycerol gelatin matrix of heating and melting, keep 78-102 ℃ of steady temperature, splash in the methyl-silicone oil, collect drop pill promptly with dropper.
12, support the preparation method of the oral preparation of Chinese traditional medicinal of lung according to claim 4 or 5 described nourishing kidneys, it is characterized in that: the preparation technology of described pill is: Poria partly or entirely is ground into the above fine powder of 100 orders; Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, by macroporous adsorptive resins, carry out eluting with the ethanol of 50-80%, reclaim ethanol, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni thick paste and Poria powder mix, and carry out spray drying, pulverize, cross the above screen cloth of 60 orders and 60 orders, add the paeonol mix homogeneously, add the refined honey that medicated powder amount 0.3-1 doubly measures again and close medicine, make honeyed pill and get final product.
13, support the preparation method of the oral preparation of Chinese traditional medicinal of lung according to claim 4 or 5 described nourishing kidneys, it is characterized in that: the preparation technology of described tablet is: Poria partly or entirely is ground into the above fine powder of 100 orders; The Cortex Moutan steam distillation, distillate carries out enclose with the cyclodextrin that 0.5-5 doubly measures, and the clathrate cold drying is ground into fine powder promptly; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filter, merging filtrate also is concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, Radix Ophiopogonis, Fructus Schisandrae Chinensis, medicinal residues behind the Fructus Corni ethanol extraction and remaining Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, the decocting that Rhizoma Alismatis adding 3-10 doubly measures boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, pass through macroporous adsorptive resins, ethanol with 50-80% carries out eluting, reclaim ethanol, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis again, Fructus Schisandrae Chinensis, Fructus Corni thick paste and Poria powder mix, and carry out spray drying, add recipe quantity 5-20% starch and above-mentioned paeonol cyclodextrin clathrate mixing, use alcohol granulation, add dried grain amount 0.5-1% magnesium stearate mixing again, tabletting, with the 2-8% film coating agent plain sheet is carried out coating, promptly get tablet.
14, support the preparation method of the oral preparation of Chinese traditional medicinal of lung according to claim 4 or 5 described nourishing kidneys, it is characterized in that: the preparation technology of described granule is: Cortex Moutan water vapour distillation paeonol; Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni alcohol reflux 1-5 time, each 0.5-5 hour, filtration, merging filtrate also are concentrated into the thick paste shape, with aqueous solution and the medicinal residues after the Cortex Moutan distillation, the decocting that medicinal residues behind Radix Ophiopogonis, Fructus Schisandrae Chinensis, the Fructus Corni ethanol extraction and Poria, Rhizoma Dioscoreae, Radix Rehmanniae Preparata, Rhizoma Alismatis adding 3-10 doubly measure boils 1-5 time, each 1-5 hour, extracting solution filters, merging filtrate, be concentrated into the thick paste shape, add above-mentioned Radix Ophiopogonis, Fructus Schisandrae Chinensis, Fructus Corni thick paste again, the auxiliary materials and mixing that paeonol and 2-5 doubly measure is granulated, and promptly gets granule; The employed adjuvant of granule of the present invention comprises dextrin, sucrose, sweeting agent or lactose.
15, support the preparation method of the oral preparation of Chinese traditional medicinal of lung according to claim 4 or 5 described nourishing kidneys, it is characterized in that: the preparation technology of described oral liquid is: the Cortex Moutan steam distillation, collect distillate promptly; The decocting that remaining medicine adding 3-10 doubly measures boils 1-5 time, and each 1-5 hour, extracting solution filtered, merging filtrate filters, and filtrate concentrates, left standstill 10-50 hour, and got supernatant, filter, filtrate continuation concentrates, and cold preservation 10-50 hour, filters filtrate adding sucrose and makes final sugar content 5-60%, boil dissolving, filter, add the 0.06%-0.8% sodium benzoate, add water to full dose, stir evenly, promptly.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103860565A (en) * | 2012-12-10 | 2014-06-18 | 中国人民解放军军事医学科学院毒物药物研究所 | Medicinal composition for treating diabetes hepatic fibrosis |
| CN105343498A (en) * | 2014-12-26 | 2016-02-24 | 杭州美誉医药有限公司 | Traditional Chinese medicine preparation for moistening lungs and preparation method and uses thereof |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102225152B (en) * | 2011-06-23 | 2012-07-25 | 贵州弘康药业有限公司 | Preparation method of Maiwei rehmannia oral preparation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1362229A (en) * | 2001-01-08 | 2002-08-07 | 杨孟君 | Nano Maiwei Rehmannine Praeparatae medicine and its preparation |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103860565A (en) * | 2012-12-10 | 2014-06-18 | 中国人民解放军军事医学科学院毒物药物研究所 | Medicinal composition for treating diabetes hepatic fibrosis |
| CN105343498A (en) * | 2014-12-26 | 2016-02-24 | 杭州美誉医药有限公司 | Traditional Chinese medicine preparation for moistening lungs and preparation method and uses thereof |
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