CN106539892B - Radix ranunculi ternati traditional Chinese medicine composition as well as preparation method and application thereof - Google Patents
Radix ranunculi ternati traditional Chinese medicine composition as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention provides a radix ranunculi ternati traditional Chinese medicine composition, which consists of radix ranunculi ternati extract and propolis; the mass ratio of the ternate buttercup root extract to the propolis to the centella asiatica extract is 3-6: 4-1: 4 to 1. The composition has effective anti-inflammatory effect by utilizing synergistic effect of radix Ranunculi Ternati, propolis and herba Centellae. The invention also provides application of the radix ranunculi ternati traditional Chinese medicine composition in preparation of anti-inflammatory medicines. The invention also provides a preparation method of the radix ranunculi ternati traditional Chinese medicine composition, which comprises the following steps: (1) cutting propolis into pieces, adding starch with equal weight, pulverizing with a pulverizer, and sieving with 80 mesh sieve to obtain propolis powder; (2) mixing a radix ranunculi ternati extract, propolis powder and a centella asiatica extract in a mass ratio of (3-6): 4-1: 4-1, and sieving by a 80-mesh sieve to obtain the ternate buttercup root traditional Chinese medicine composition. The preparation process is simple and suitable for industrial production.
Description
Technical Field
The invention relates to the field of pharmaceutical compositions, and in particular relates to a radix ranunculi ternati traditional Chinese medicine composition as well as a preparation method and application thereof.
Background
Inflammation, a defense response of living tissues having a vascular system to injury factors, is a defense response of the body to stimuli, and is manifested by redness, swelling, heat, pain, and dysfunction. Vascular responses are a central link in the inflammatory process. The inflammation may be infectious inflammation caused by infection or non-infectious inflammation not caused by infection. The response presented by inflammation to damaged local tissues of the body is called inflammatory response, which is divided into acute and chronic according to the duration; major tissue changes can be classified into degenerative inflammation, exudative inflammation, proliferative inflammation and specific inflammation. The basic pathological changes of inflammation are summarized as degeneration, exudation and proliferation of local tissues. Inflammation is usually beneficial and an automatic defense response of the human body, but sometimes it is also harmful. Inflammatory response is the basis of some diseases, such as severe hypersensitivity inflammation which can threaten the life of a patient, in addition, inflammation occurring in a special part or organ can cause serious consequences, such as brain inflammation which can press the vital center, vocal cord inflammation which blocks the throat to cause asphyxiation, severe myocarditis which can affect the heart function, and the like, and at this time, an anti-inflammatory drug should be used to inhibit the inflammatory response.
There are two main classes of anti-inflammatory drugs currently in use: one is a steroidal anti-inflammatory drug, namely glucocorticoid hydrocortisone secreted by adrenal cortex and artificially synthesized derivatives thereof; another class is non-steroidal anti-inflammatory drugs, which are antipyretic, analgesic and anti-inflammatory drugs in medical practice such as aspirin, phenylbutazone, celecoxib, etc. However, the above drugs are easy to have side effects such as upper gastrointestinal hemorrhage, lethargy, absentmindedness, mental depression, platelet aggregation, hepatotoxicity, anaphylaxis and the like caused by gastric and duodenal ulcer while resisting inflammation. The traditional Chinese medicine has the advantages of low adverse reaction incidence rate, abundant resources and the like, and with the gradual and deep research on the anti-inflammatory action of the traditional Chinese medicine in modern medicine, the result proves that the pharmacological activity of the anti-inflammatory action of some traditional Chinese medicines comprises multi-path anti-inflammatory action, such as action on inflammatory cells, action on inflammatory molecules, action on cytokines and cell second messengers and the like.
Chinese medicine Radix Ranunculi Ternati (Radix Ranunculus ternati) is dried root tuber of Ranunculus japonicus (Ranunculus ternatus Thunb) of Ranunculaceae, and is also called: san scattered grass (Zhejiang), radix ranunculi ternati (Henan), radix pini sylvestris (Anhui), and flos chrysanthemi (Guangxi) are newly found herbal medicines in Xinyang areas in Henan in the 50 th century in 20 th century, and all provinces of China are distributed, and the text records are firstly found in a Chinese herbal medicine manual, and are collected and carried in the pharmacopoeia of the people's republic of China from the 1977 edition. The radix ranunculi ternati is warm in nature, sweet and pungent in taste, enters liver and lung channels, has the effects of clearing heat and removing toxicity, reducing swelling and resolving masses, relieving cough and eliminating phlegm and the like, and is clinically used for treating diseases such as phthisis, tuberculous lymphadenitis, sphagitis, malaria and the like.
Centella asiatica is dry whole herb of Centella asiatica (L.) asiatica of Umbelliferae, has bitter, pungent and cold taste, and has effects of clearing heat, promoting diuresis, removing toxic substance and relieving swelling, and can be used for treating jaundice due to damp-heat pathogen, heatstroke diarrhea, stranguria with stone, carbuncle, sore, and traumatic injury. Researches on Mingzhi, etc. find that the asiaticoside has a good inhibiting effect on rat cotton ball granuloma and a good inhibiting effect on mouse ear swelling caused by xylene.
The propolis is a dry secretion of Apis mellifera L. of Apidae, is bitter, pungent and cold in nature, enters spleen and stomach channels, contains abundant bioactive substances beneficial to human health, has multiple effects, can diminish inflammation, resist bacteria, enhance immunity, relieve itching, resist oxidation, reduce blood sugar, reduce blood fat, resist radiation damage and the like, and is a precious natural resource. Some anti-inflammatory substances have been isolated from propolis, among which caffeic acid, quercetin, naringenin, caffeic acid phenethyl ester contribute to inhibition of synthesis of prostaglandins and leukotrienes by macrophages, and have inhibitory effects on myeloperoxidase activity, NADPH-oxidase, ornithine decarboxylase, and tyrosine protein kinase. Krol et al attribute anti-inflammatory activity to compounds such as salicylic acid, apigenin, ferulic acid and galangin. Naito found that the propolis ointment had an effect of improving the swelling of hind paw in rats induced by carrageenan, while suppressing the chemotaxis of neutrophils. Sosa determined the effect of 3 commercial propolis preparations on improving croton oil-induced ear swelling in mice, and the results showed that all propolis preparations exhibited significant anti-swelling effects, with the effects appearing as a dose relationship. The Santos researches the effect of Brazilian propolis preparation on clinical gingival gingivitis, and test results show that the propolis preparation can effectively eliminate the palate edema and erythema of teeth. Paulino measures the effect of the Brazilian green glue extract and the Bulgaria propolis extract on improving the inflammation of rats under different stimulation conditions, and the results show that both the two kinds of propolis can preventively improve the inflammation caused by injecting formaldehyde into hind feet of the mice; the Brazilian green gum extract can relieve foot swelling induced by Freund's complete adjuvant and serotonin stimulation, relieve ear swelling induced by capsaicin, relieve peritonitis induced by carrageenan, and reduce transfer of leukocyte, mainly neutrophil, in peritoneal fluid. Poplar and the like observe the influence of the propolis aqueous solution prepared by the gastric perfusion alcohol-soluble water extraction method on the capillary permeability of mice and the ear swelling caused by dimethylbenzene, and find that the propolis aqueous solution prepared by the alcohol-soluble water extraction method has obvious anti-inflammatory effect.
Disclosure of Invention
The invention aims to provide a radix ranunculi ternati traditional Chinese medicine composition which can play an effective anti-inflammatory role by utilizing the synergistic effect of the radix ranunculi ternati, propolis and centella asiatica.
A Chinese medicinal composition comprises radix Ranunculi Ternati extract, propolis, and herba Centellae extract; the mass ratio of the ternate buttercup root extract to the propolis to the centella asiatica extract is 3-6: 4-1: 4 to 1.
The invention combines the catclaw buttercup root with the propolis and the asiatic centella, has better anti-inflammatory effect and has better effect than that of a single catclaw buttercup root or a single propolis or a single asiatic centella extract.
Preferably, in the radix ranunculi ternati traditional Chinese medicine composition, the mass ratio of the radix ranunculi ternati extract to the propolis to the centella asiatica extract is 4:3: 3.
Edible or medicinal auxiliary materials can be added into the raw materials, and the method comprises the following steps: at least one of an excipient, a flavoring agent, a diluent, a suspending agent, an antioxidant, an emulsifier, a thickener, a solubilizer, a filler, a lubricant, a humectant, a disintegrant, an adhesive, and a preservative.
The invention also aims to provide application of the ternate buttercup root traditional Chinese medicine composition in preparing anti-inflammatory medicines.
The invention also provides a preparation method of the radix ranunculi ternati traditional Chinese medicine composition, which comprises the following steps:
(1) cutting propolis into pieces, adding starch with equal weight, pulverizing with a pulverizer, and sieving with 80 mesh sieve to obtain propolis powder;
(2) mixing a radix ranunculi ternati extract, propolis powder and a centella asiatica extract in a mass ratio of (3-6): 4-1: 4-1, and sieving by a 80-mesh sieve to obtain the ternate buttercup root traditional Chinese medicine composition.
Preferably, the preparation method of the ternate buttercup root traditional Chinese medicine composition comprises the following steps:
(1) cutting propolis into pieces, adding starch with equal weight, pulverizing with a pulverizer, and sieving with 80 mesh sieve to obtain propolis powder;
(2) the ternate buttercup root extract and the propolis powder are uniformly mixed according to the mass ratio of 4:3:3, and are sieved by a 80-mesh sieve to obtain the ternate buttercup root Chinese medicinal composition.
The preparation method of the radix ranunculi ternati traditional Chinese medicine composition further comprises the following steps: and (3) directly adding the product prepared in the step (2) or adding edible or medicinal auxiliary materials, and further preparing the product into capsules, tablets, granules, oral liquid or powder by a conventional pharmaceutical method.
The ternate buttercup root extract can be a commercially available product or can be prepared by itself, and the preparation method can be carried out according to a method recorded in Chinese herbal medicine component chemistry, and specifically comprises the following steps: pulverizing radix Ranunculi Ternati, reflux-extracting with ethanol, refrigerating, filtering, concentrating the filtrate, and vacuum drying to obtain radix Ranunculi Ternati extract.
The mass percentage content of the total saponins in the ternate buttercup root extract is 30-70%;
the propolis is pure propolis, is extracted by ethanol, can adopt a commercially available product, can also be prepared by self, and specifically comprises the following steps: extracting propolis raw material with ethanol under reflux, filtering, removing lead, concentrating, and vacuum drying to obtain pure propolis.
The mass percentage of the total flavone in the propolis is 8 to 15 percent;
the centella asiatica extract can be prepared by a commercially available product or a self-prepared product, and the preparation method can be carried out according to a method recorded in Chinese herbal medicine component chemistry, and specifically comprises the following steps: pulverizing herba Centellae, reflux-extracting with ethanol, refrigerating, filtering, concentrating the filtrate, and vacuum drying to obtain herba Centellae extract.
The mass percentage of the total saponins in the centella asiatica is 30-70%.
The radix ranunculi ternati, the propolis and the centella asiatica meet the quality standards of the radix ranunculi ternati, the propolis and the centella asiatica in pharmacopoeia of the people's republic of China. Radix Ranunculi Ternati is dry root tuber of Ranunculus ternatus Thunb of Ranunculaceae, has effects of eliminating phlegm and resolving hard mass, removing toxic substance and relieving swelling, and can be used for treating lymphoid tuberculosis, subcutaneous nodule, furuncle and pyogenic infections. Propolis is solid jelly with viscosity prepared by mixing plant resin collected from Italian bee of Apidae and secretions such as jawbone and cerifera, and has effects of tonifying asthenia, eliminating turbid lipid, and quenching thirst, and can be used for treating asthenia, senilism, hyperlipidemia, and diabetes. Centella asiatica is dry whole herb of Centella asiatica (L.) urban of Umbelliferae, has effects of clearing heat, promoting diuresis, removing toxic substance and relieving swelling, and can be used for treating damp-heat jaundice, sunstroke diarrhea, stranguria with stone, carbuncle, swelling, sore, and traumatic injury. Compared with the prior art, the invention has the following beneficial effects:
compared with the existing traditional Chinese medicine anti-inflammatory drugs, the composition of the invention greatly improves the anti-inflammatory effect, and no product with the composition exists at home and abroad at present. Compared with the same dosage of single-flavor ternate buttercup root, single-flavor propolis or single-flavor centella asiatica, the composition disclosed by the invention has better anti-inflammatory effect by utilizing the synergistic effect of the ternate buttercup root, the propolis and the centella asiatica. The composition of the invention has simple preparation process and is suitable for industrial production.
Detailed Description
Example 1
Crushing the radix ranunculi ternati, sieving by a 60-mesh sieve, heating and refluxing for 2 times by using 85% ethanol with the volume of 6-8 times, extracting for 3 hours each time, collecting and combining extracting solutions, concentrating, and drying to obtain the radix ranunculi ternati extract, wherein the mass percentage content of total saponins in the radix ranunculi ternati extract is 50-70%.
The propolis is smashed into blocks, starch with the same weight is added, the blocks are smashed by a smashing machine and sieved by an 80-mesh sieve, the propolis powder with the grain size smaller than 80 meshes is obtained, and the mass percentage of total flavone in the propolis powder is 8% -15%.
The preparation method comprises the steps of crushing the centella, sieving by a 60-mesh sieve, heating and refluxing the centella by using 75% ethanol with the volume being 8-10 times of that of the centella for 2 times, collecting and combining extracting solutions, concentrating the extracting solutions, and drying the extracting solutions to obtain the centella extract, wherein the mass percentage of the total saponins in the centella is 50% -70%.
Mixing radix Ranunculi Ternati extract, propolis powder and herba Centellae extract at a mass ratio of 4:3:3, sieving with 80 mesh sieve to obtain radix Ranunculi Ternati Chinese medicinal composition powder with particle size less than 80 mesh, and encapsulating. The capsule is irradiated and sterilized to prepare the ternate buttercup root Chinese medicinal composition capsule.
Example 2
Crushing the radix ranunculi ternati, sieving by a 60-mesh sieve, heating and refluxing for 2 times by using 85% ethanol with the volume of 6-8 times, extracting for 3 hours each time, collecting and combining extracting solutions, concentrating, and drying to obtain the radix ranunculi ternati extract, wherein the mass percentage content of total saponins in the radix ranunculi ternati extract is 30-50%.
The propolis is smashed into blocks, starch with the same weight is added, the blocks are smashed by a smashing machine and sieved by an 80-mesh sieve, the propolis powder with the grain size smaller than 80 meshes is obtained, and the mass percentage of total flavone in the propolis powder is 8% -15%.
The preparation method comprises the steps of crushing the centella, sieving by a 60-mesh sieve, heating and refluxing the centella by using 75% ethanol with the volume being 8-10 times of that of the centella for 2 times, collecting and combining extracting solutions, concentrating the extracting solutions, and drying the extracting solutions to obtain the centella extract, wherein the mass percentage of the total saponins in the centella is 30-50%.
Mixing radix Ranunculi Ternati extract, propolis powder and herba Centellae extract at a mass ratio of 6:2:2, sieving with 80 mesh sieve to obtain radix Ranunculi Ternati Chinese medicinal composition powder with particle size less than 80 mesh, and encapsulating. The capsule is irradiated and sterilized to prepare the ternate buttercup root Chinese medicinal composition capsule.
Example 3
Crushing the radix ranunculi ternati, sieving by a 60-mesh sieve, heating and refluxing for 2 times by using 85% ethanol with the volume of 6-8 times, extracting for 3 hours each time, collecting and combining extracting solutions, concentrating, and drying to obtain the radix ranunculi ternati extract, wherein the mass percentage content of total saponins in the radix ranunculi ternati extract is 50-70%.
The propolis is smashed into blocks, starch with the same weight is added, the blocks are smashed by a smashing machine and sieved by an 80-mesh sieve, the propolis powder with the grain size smaller than 80 meshes is obtained, and the mass percentage of total flavone in the propolis powder is 8% -15%.
The preparation method comprises the steps of crushing the centella, sieving by a 60-mesh sieve, heating and refluxing the centella by using 75% ethanol with the volume being 8-10 times of that of the centella for 2 times, collecting and combining extracting solutions, concentrating the extracting solutions, and drying the extracting solutions to obtain the centella extract, wherein the mass percentage of the total saponins in the centella is 30-50%.
Mixing radix ranunculi ternati extract, propolis powder and herba Centellae extract at a mass ratio of 5:3:2, sieving with 80 mesh sieve to obtain radix ranunculi ternati Chinese medicinal composition powder with particle size less than 80 mesh, and encapsulating. The capsule is irradiated and sterilized to prepare the ternate buttercup root Chinese medicinal composition capsule.
Example 4
Crushing the radix ranunculi ternati, sieving by a 60-mesh sieve, heating and refluxing for 2 times by using 85% ethanol with the volume of 6-8 times, extracting for 3 hours each time, collecting and combining extracting solutions, concentrating, and drying to obtain the radix ranunculi ternati extract, wherein the mass percentage content of total saponins in the radix ranunculi ternati extract is 40-60%.
The propolis is smashed into blocks, starch with the same weight is added, the blocks are smashed by a smashing machine and sieved by an 80-mesh sieve, the propolis powder with the grain size smaller than 80 meshes is obtained, and the mass percentage of total flavone in the propolis powder is 8% -15%.
The preparation method comprises the steps of crushing the centella, sieving by a 60-mesh sieve, heating and refluxing the centella by using 75% ethanol with the volume being 8-10 times of that of the centella for 2 times, collecting and combining extracting solutions, concentrating the extracting solutions, and drying the extracting solutions to obtain the centella extract, wherein the mass percentage of the total saponins in the centella is 40-60%.
Mixing radix Ranunculi Ternati extract, propolis powder and herba Centellae extract at a mass ratio of 3:4:3, sieving with 80 mesh sieve to obtain radix Ranunculi Ternati Chinese medicinal composition powder with particle size less than 80 mesh, and encapsulating. The capsule is irradiated and sterilized to prepare the ternate buttercup root Chinese medicinal composition capsule.
Example 5
Crushing the radix ranunculi ternati, sieving by a 60-mesh sieve, heating and refluxing for 2 times by using 85% ethanol with the volume of 6-8 times, extracting for 3 hours each time, collecting and combining extracting solutions, concentrating, and drying to obtain the radix ranunculi ternati extract, wherein the mass percentage content of total saponins in the radix ranunculi ternati extract is 50-70%.
The propolis is smashed into blocks, starch with the same weight is added, the blocks are smashed by a smashing machine and sieved by an 80-mesh sieve, the propolis powder with the grain size smaller than 80 meshes is obtained, and the mass percentage of total flavone in the propolis powder is 8% -15%.
The preparation method comprises the steps of crushing the centella, sieving by a 60-mesh sieve, heating and refluxing the centella by using 75% ethanol with the volume being 8-10 times of that of the centella for 2 times, collecting and combining extracting solutions, concentrating the extracting solutions, and drying the extracting solutions to obtain the centella extract, wherein the mass percentage of the total saponins in the centella is 50% -70%.
Uniformly mixing the ternate buttercup root extract, the propolis powder and the centella asiatica extract according to the mass ratio of 4:3:3, sieving by a 80-mesh sieve to obtain ternate buttercup root Chinese medicinal composition powder with the particle size of less than 80 meshes, and mixing the ternate buttercup root Chinese medicinal composition powder and cellulose according to the mass ratio of 1: 1, fully mixing, tabletting, coating, and preparing into tablets, and performing irradiation sterilization on the tablets to prepare the ternate buttercup root Chinese medicinal composition tablets.
Example 6
Crushing the radix ranunculi ternati, sieving by a 60-mesh sieve, heating and refluxing for 2 times by using 85% ethanol with the volume of 6-8 times, extracting for 3 hours each time, collecting and combining extracting solutions, concentrating, and drying to obtain the radix ranunculi ternati extract, wherein the mass percentage content of total saponins in the radix ranunculi ternati extract is 30-50%.
The propolis is smashed into blocks, starch with the same weight is added, the blocks are smashed by a smashing machine and sieved by an 80-mesh sieve, the propolis powder with the grain size smaller than 80 meshes is obtained, and the mass percentage of total flavone in the propolis powder is 8% -15%.
The preparation method comprises the steps of crushing the centella, sieving by a 60-mesh sieve, heating and refluxing the centella by using 75% ethanol with the volume being 8-10 times of that of the centella for 2 times, collecting and combining extracting solutions, concentrating the extracting solutions, and drying the extracting solutions to obtain the centella extract, wherein the mass percentage of the total saponins in the centella is 40-60%.
Uniformly mixing the ternate buttercup root extract, the propolis powder and the centella asiatica extract according to the mass ratio of 6:2:2, sieving by a 80-mesh sieve to obtain ternate buttercup root Chinese medicinal composition powder with the particle size of less than 80 meshes, and mixing the ternate buttercup root Chinese medicinal composition powder and cellulose according to the mass ratio of 1: 1, fully mixing, tabletting, coating, and preparing into tablets, and performing irradiation sterilization on the tablets to prepare the ternate buttercup root Chinese medicinal composition tablets.
Example 7
Crushing the radix ranunculi ternati, sieving by a 60-mesh sieve, heating and refluxing for 2 times by using 85% ethanol with the volume of 6-8 times, extracting for 3 hours each time, collecting and combining extracting solutions, concentrating, and drying to obtain the radix ranunculi ternati extract, wherein the mass percentage content of total saponins in the radix ranunculi ternati extract is 40-60%.
The propolis is smashed into blocks, starch with the same weight is added, the blocks are smashed by a smashing machine and sieved by an 80-mesh sieve, the propolis powder with the grain size smaller than 80 meshes is obtained, and the mass percentage of total flavone in the propolis powder is 8% -15%.
The preparation method comprises the steps of crushing the centella, sieving by a 60-mesh sieve, heating and refluxing the centella by using 75% ethanol with the volume being 8-10 times of that of the centella for 2 times, collecting and combining extracting solutions, concentrating the extracting solutions, and drying the extracting solutions to obtain the centella extract, wherein the mass percentage of the total saponins in the centella is 40-60%.
Uniformly mixing the ternate buttercup root extract, the propolis powder and the centella asiatica extract according to the mass ratio of 5:3:2, sieving by a 80-mesh sieve to obtain ternate buttercup root Chinese medicinal composition powder with the particle size of less than 80 meshes, and mixing the ternate buttercup root Chinese medicinal composition powder and cellulose according to the mass ratio of 1: 1, fully mixing, tabletting, coating, and preparing into tablets, and performing irradiation sterilization on the tablets to prepare the ternate buttercup root Chinese medicinal composition tablets.
Example 8
Crushing the radix ranunculi ternati, sieving by a 60-mesh sieve, heating and refluxing for 2 times by using 85% ethanol with the volume of 6-8 times, extracting for 3 hours each time, collecting and combining extracting solutions, concentrating, and drying to obtain the radix ranunculi ternati extract, wherein the mass percentage content of total saponins in the radix ranunculi ternati extract is 40-60%.
The propolis is smashed into blocks, starch with the same weight is added, the blocks are smashed by a smashing machine and sieved by an 80-mesh sieve, the propolis powder with the grain size smaller than 80 meshes is obtained, and the mass percentage of total flavone in the propolis powder is 8% -15%.
The preparation method comprises the steps of crushing the centella, sieving by a 60-mesh sieve, heating and refluxing the centella by using 75% ethanol with the volume being 8-10 times of that of the centella for 2 times, collecting and combining extracting solutions, concentrating the extracting solutions, and drying the extracting solutions to obtain the centella extract, wherein the mass percentage of the total saponins in the centella is 50% -70%.
Uniformly mixing the ternate buttercup root extract, the propolis powder and the centella asiatica extract according to the mass ratio of 3:4:3, sieving by a 80-mesh sieve to obtain ternate buttercup root Chinese medicinal composition powder with the particle size of less than 80 meshes, and mixing the ternate buttercup root Chinese medicinal composition powder and cellulose according to the mass ratio of 1: 1, fully mixing, tabletting, coating, and preparing into tablets, and performing irradiation sterilization on the tablets to prepare the ternate buttercup root Chinese medicinal composition tablets.
Animal experiments show that the traditional Chinese medicine composition prepared in the examples 1-8 has an obvious anti-inflammatory effect. The following description will be made in detail by taking the traditional Chinese medicine composition of radix ranunculi ternati prepared in example 1 as an example.
The anti-inflammatory effect of the radix ranunculi ternati traditional Chinese medicine composition is compared with that of single radix ranunculi ternati or single propolis or single centella asiatica:
the radix ranunculi ternati traditional Chinese medicine composition mainly comprises a radix ranunculi ternati extract, propolis and a centella asiatica extract, the effect of the radix ranunculi ternati traditional Chinese medicine composition is observed by comparing the effects of the radix ranunculi ternati traditional Chinese medicine composition and a single radix ranunculi ternati extract, a single propolis and a single centella asiatica extract on the permeability of capillaries in abdominal cavities of mice, the effects of the single herba ranunculi ternati extract on ear swelling of the mice caused by xylene and the effects of the single herba ranunculi ternati chinensis extract on foot swelling of rats caused by:
1. material
1.1 the radix ranunculi ternati traditional Chinese medicine composition prepared in example 1 is black powder, in this experiment, distilled water is used as a solvent, the radix ranunculi ternati traditional Chinese medicine composition prepared in example 1 is prepared into a test solution, and a single-component radix ranunculi ternati extract test solution, a single-component propolis test solution, a single-component herba centellae extract test solution, an equal amount of distilled water control solution and an aspirin control group are arranged.
1.2 experimental animals and detection conditions, wherein the license number used by the experimental animals is as follows: SYXK (Zhe 2014-. ICR mice for experiments, clean grade, weight of 20 +/-2 g, each half of male and female, Wistar rat clean grade, weight of 200 +/-2 g, male, provided by Shanghai Si Laike laboratory animal Limited liability company, and production licenses of laboratory animals are as follows: SCXK (Shanghai) 2012-0002. The feed is provided by Experimental animal center in Zhejiang province, and has the execution standard GB 14924-2001. Detecting the environmental conditions, wherein the temperature range is 20-24 ℃, and the relative humidity range is 40-70%. Animals were acclimated in the animal house environment for 3 days prior to the experiment.
1.3 dosage design, the recommended amount of the ternate buttercup root Chinese medicinal composition prepared in example 1 is 1.5 g/day/60 kg of body weight, the experiment shows that a low-dose group of the ternate buttercup root Chinese medicinal composition is 0.75g/kg (equivalent to 30 times of the recommended amount of a human body), a high-dose group of the ternate buttercup root Chinese medicinal composition is 1.5g/kg (equivalent to 60 times of the recommended amount of the human body), a ternate buttercup root extract group is 0.6g/kg, a propolis group is 0.45g/kg, a centella asiatica extract group is 0.45g/kg, a single-flavor ternate buttercup root extract, a single-flavor propolis, a single-flavor centella asiatica and a high-dose group of the ternate buttercup root Chinese medicinal composition are the same in corresponding drug dosage, a negative control group is 20ml/kg, distilled water is used, and a positive control group is 0..
1.4 the main instruments comprise an electronic balance, a centrifugal machine, an adjustable pipettor, a full-wavelength microplate reader and the like.
2. The experimental method comprises the following steps:
2.1 Effect on mouse peritoneal capillary permeability: 70 male ICR mice were divided into 6 groups at random by body weight, and the subjects were gavaged 1 time a day for 5 consecutive days. 30min after the last administration, the tail of the mouse is injected with 0.1ml/10g body weight of 2% Evans blue physiological saline solution intravenously, 0.1 ml/mouse is injected with 0.6% acetic acid solution intraperitoneally, the cervical vertebra is removed after 30min, the mouse is killed, the abdominal cavity of the mouse is washed by 5ml physiological saline, the washing liquid is collected, the supernatant is obtained by centrifugation at 3000rpm, the color is compared at 590nm wavelength, the capillary permeability of the abdominal cavity of the mouse is judged according to the absorbance (OD value), and the inhibition rate (%) is calculated.
2.2 effects of p-xylene on ear swelling in mice: 70 female ICR mice were divided into 6 groups by weight, and each group was gavaged 1 time a day for 5 consecutive days. 30min after the last administration, 20 μ l of xylene was applied dropwise to the left ear, the right ear was left untreated to serve as a self-control, the mouse was sacrificed by removing the cervical vertebrae after 30min, the identical part of both ears was cut off in an equal area using a punch with a diameter of 8mm, the mouse was weighed on an electronic balance, the difference between the weight of the left ear and the weight of the right ear was taken as the degree of swelling, the differences of the groups were compared, and the swelling inhibition (%) was calculated.
2.3 Effect on carrageenan-induced footpad swelling in rats: 70 male Wistar rats were divided into 6 groups by weight, and the test subjects were administered by gavage 1 time a day for 5 consecutive days. The right hind ankle joint of each mouse was marked before the experiment, and the foot volume of each mouse was measured 2 times by the foot volume method, and the average value was taken as the foot volume before administration to each mouse. 30min after the last administration, subcutaneously injecting 1% carrageenan solution into the right hind foot sole of each rat to cause inflammation, measuring the volume of the right hind foot by the same method for 1, 2, 3, 4, 5 and 6h after causing inflammation respectively, calculating the change value of the volume of the hind foot sole of the rat before and after causing inflammation, and judging the anti-inflammatory effect of the drug by the swelling degree.
2.3 data processing: analysis was performed using SPSS17.0 statistical software.
3. Results of the experiment
3.1 influence of the traditional Chinese medicine composition of radix ranunculi ternati on the permeability of capillary vessels in abdominal cavity of mice:
the results are shown in Table 1. The radix ranunculi ternati traditional Chinese medicine composition has obvious inhibition effects on the increase of permeability of capillary vessels in abdominal cavities of mice caused by acetic acid in a high-dose and low-dose group, a radix ranunculi ternati extract group, a propolis group and a centella asiatica extract group, and the differences are all significant (P is less than 0.05); compared with the radix ranunculi ternati extract group, the propolis group and the centella asiatica extract group, the OD values of the high-dose and low-dose radix ranunculi ternati traditional Chinese medicine composition groups are obviously reduced (P is less than 0.05); the inhibition rate of the radix ranunculi ternati traditional Chinese medicine composition group is 27.5-28.8%, and is higher than that of the radix ranunculi ternati extract group, the propolis group and the centella asiatica extract group.
TABLE 1 Effect of traditional Chinese medicine composition of radix Ranunculi Ternati and single medicine on permeability of capillary blood vessel in abdominal cavity of mouse
Note that P < 0.05 in comparison with the negative control group, # 0.05 in comparison with the Ranunculus ternatus extract group, ▲ < 0.05 in comparison with the propolis group, and △ < 0.05 in comparison with the centella asiatica group.
3.2 the effect of the traditional Chinese medicine composition of radix ranunculi ternati on the mouse ear swelling caused by xylene:
the results are shown in Table 2. The radix ranunculi ternati traditional Chinese medicine composition has obvious inhibition effects on mouse ear swelling caused by xylene in high and low dose groups, radix ranunculi ternati extract groups, propolis groups and centella asiatica extract groups, and the differences are significant (P is less than 0.05); compared with the radix ranunculi ternati extract group, the high-dose group of the radix ranunculi ternati traditional Chinese medicine composition has obvious difference (P is less than 0.05); compared with the propolis group and the asiatic pennywort herb extract group, the radix ranunculi ternati traditional Chinese medicine composition has obvious difference in high and low dose groups (P is less than 0.05); the inhibition rate of the radix ranunculi ternati traditional Chinese medicine composition group is 23.5-29.0%, and the inhibition rate is higher than that of the radix ranunculi ternati extract group, the propolis group and the centella asiatica extract group.
TABLE 2 Effect of traditional Chinese medicine composition of radix Ranunculi Ternati and single medicine on mouse ear swelling caused by xylene
Note that P < 0.05 in comparison with the negative control group, # 0.05 in comparison with the Ranunculus ternatus extract group, ▲ < 0.05 in comparison with the propolis group, and △ < 0.05 in comparison with the centella asiatica group.
3.3 Effect of the traditional Chinese medicine composition of radix ranunculi ternati on rat plantar swelling caused by carrageenan:
the results are shown in tables 3 and 4. The 2h swelling degree of the high-dose group of the radix ranunculi ternati traditional Chinese medicine composition is obviously lower than that of the negative control group
Compared with a negative control group, the swelling degree of the radix ranunculi ternati traditional Chinese medicine composition in high and low dose groups within 3-6 h is remarkably reduced (P is less than 0.05); swelling degree of the radix ranunculi ternati extract group, the propolis group and the centella asiatica extract group within 3-6 hours is obviously reduced (P is less than 0.05) compared with that of a negative control group; compared with the radix ranunculi ternati extract group, the propolis group and the centella asiatica extract group, the swelling degree of the radix ranunculi ternata traditional Chinese medicine composition in the high-dose group within 2-6 h is remarkably reduced (P is less than 0.05), and the inhibition effect of the radix ranunculi ternati traditional Chinese medicine composition on the sole swelling of rats caused by carrageenan is better than that of the radix ranunculi ternati extract group, the propolis group and the centella asiatica extract group.
TABLE 3 Effect of traditional Chinese medicine composition of radix Ranunculi Ternati and single medicine on swelling of rat foot sole
Note that P < 0.05 in comparison with the negative control group, # 0.05 in comparison with the Ranunculus ternatus extract group, ▲ < 0.05 in comparison with the propolis group, and △ < 0.05 in comparison with the centella asiatica group.
TABLE 4 Effect of traditional Chinese medicine composition of radix Ranunculi Ternati and single medicine on swelling of rat foot sole
Note that P < 0.05 in comparison with the negative control group, # 0.05 in comparison with the Ranunculus ternatus extract group, ▲ < 0.05 in comparison with the propolis group, and △ < 0.05 in comparison with the centella asiatica group.
4. And (3) knotting:
the experimental result shows that compared with a negative control group (distilled water), each dosage group of the ternate buttercup root traditional Chinese medicine composition can inhibit the permeability increase of the capillary vessel of the abdominal cavity of a mouse caused by acetic acid, inhibit the ear swelling of the mouse caused by dimethylbenzene and inhibit the foot swelling of a rat caused by carrageenan (P is less than 0.05). The same high dose of the radix ranunculi ternati extract, the propolis and the centella asiatica extract can also inhibit the increase of the permeability of the capillary vessels in the abdominal cavity of a mouse caused by acetic acid (P is less than 0.05), but the effect is not as strong as that of the traditional Chinese medicine composition of the radix ranunculi ternati. The same high dose of radix Ranunculi Ternati extract, propolis and herba Centellae extract can also inhibit mouse ear swelling caused by xylene (P is less than 0.05), but the effect is not as strong as that of the traditional Chinese medicine composition of radix Ranunculi Ternati. The same high dose of radix Ranunculi Ternati extract, propolis and herba Centellae extract can also inhibit carrageenan-induced foot sole swelling (P < 0.05), but the effect is not as strong as that of radix Ranunculi Ternati Chinese medicinal composition.
The traditional Chinese medicine composition of the ternate buttercup root has better anti-inflammatory effect compared with the single-ingredient ternate buttercup root extract, the single-ingredient propolis and the unit centella asiatica extract with the same dosage.
Claims (5)
1. An anti-inflammatory radix Ranunculi Ternati Chinese medicinal composition is characterized by comprising radix Ranunculi Ternati extract, propolis, and herba Centellae extract; wherein the mass ratio of the ternate buttercup root extract to the propolis to the centella asiatica extract is 4:3: 3;
the mass percentage content of the total saponins in the ternate buttercup root extract is 50-70%;
the mass percentage of the total flavone in the propolis is 8 to 15 percent;
the centella asiatica extract contains 50-70% of total saponins by mass.
2. An anti-inflammatory traditional Chinese medicine composition containing radix Ranunculi Ternati is characterized by comprising radix Ranunculi Ternati extract, propolis, herba Centellae extract, and edible or medicinal adjuvants; wherein the mass ratio of the ternate buttercup root extract to the propolis to the centella asiatica extract is 4:3: 3;
the mass percentage content of the total saponins in the ternate buttercup root extract is 50-70%;
the mass percentage of the total flavone in the propolis is 8 to 15 percent;
the centella asiatica extract contains 50-70% of total saponins by mass.
3. The use of the radix ranunculi ternati traditional Chinese medicine composition as claimed in claim 1 or 2, is characterized by comprising the application of the radix ranunculi ternati traditional Chinese medicine composition in preparing an anti-inflammatory drug.
4. The method for preparing the ternate buttercup root traditional Chinese medicine composition according to claim 1, comprising:
(1) cutting propolis into pieces, adding starch with equal weight, pulverizing with a pulverizer, and sieving with 80 mesh sieve to obtain propolis powder;
(2) the radix ranunculi ternati extract, the propolis powder and the centella asiatica extract are uniformly mixed and sieved by a 80-mesh sieve to obtain the radix ranunculi ternati traditional Chinese medicine composition.
5. The method for preparing the ternate buttercup root traditional Chinese medicine composition according to claim 2, comprising:
(1) cutting propolis into pieces, adding starch with equal weight, pulverizing with a pulverizer, and sieving with 80 mesh sieve to obtain propolis powder;
(2) mixing radix Ranunculi Ternati extract, propolis powder and herba Centellae extract, sieving with 80 mesh sieve to obtain radix Ranunculi Ternati Chinese medicinal composition, and directly or adding edible or medicinal adjuvants, and further making into capsule, tablet, granule, oral liquid or powder by conventional pharmaceutical method.
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