CN1685923A - Dairy cattle feed additive for improving milk cow production performance and its manufacturing method - Google Patents
Dairy cattle feed additive for improving milk cow production performance and its manufacturing method Download PDFInfo
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- CN1685923A CN1685923A CNA2005100099516A CN200510009951A CN1685923A CN 1685923 A CN1685923 A CN 1685923A CN A2005100099516 A CNA2005100099516 A CN A2005100099516A CN 200510009951 A CN200510009951 A CN 200510009951A CN 1685923 A CN1685923 A CN 1685923A
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Abstract
An additive of feed for increasing the milk output of milk cow is prepared through providing yeast CB, rejuvenescence, preparing its suspension, liquid culture medium, culturing, preparing solid culture medium, culturing, and drying.
Description
(1) technical field
What the present invention relates to is a kind of feed addictive, specifically a kind of feed addictive that improves ruminant animal nutrition.The invention still further relates to the preparation method of this feed addictive.
(2) background technology
For high yield cow, will descend in lactation feed intake in early stage, the energy of taking in from daily ration can not satisfy the needs of keeping with high yield, the produce power negative balance.Milk cow is employed body fat in order to keep the output of milk, causes body weight to descend.Too many loss of weight can produce adverse influence to the whole lactational performance of giving milk, and can prolong the breeding time of milk cow simultaneously, and that has reduced milk cow effectively utilizes the time limit.The high yield cow generation that particularly is under the summer high-temperature condition is more, and it is also relatively more serious to produce consequence.Under the single relatively situation of present roughage, the mode that most places take to increase the concentrated feed amount increases the output of milk, and the result produces multiple metabolic disease, and the benefit of milk cattle cultivating is reduced.How having taken good care of the cud of milk cow, made the little ecosystem in the cow rumen reach optimum state, improve the output of milk under the situation that does not change the daily ration structure, is the focus of studying both at home and abroad now.
(3) summary of the invention
The object of the present invention is to provide a kind ofly under the situation that does not change the daily ration structure, can increase the capacity usage ratio of animal to roughage, the purpose that finally reaches volume increase improves the cow feed additive of milk cow production performance.The present invention also aims to provide the preparation method of this feed addictive.
The object of the present invention is achieved like this: it is that CB yeast (Candida tropicalisBerkhout) adopts following method to cultivate resulting product:
(1) rejuvenation of spawn: use aseptic technique to change in the test tube that sterilized solid medium is housed the test tube CB barms of preserving, under 30 ℃ of constant temperatures, cultivated 3-5 days;
(2) bacteria suspension preparation: inject SPSS in cultivating the test tube that finishes, the injection rate of SPSS is the 1/4-1/3 of test tube volume, and vibration is washed, up to the physiological saline muddiness;
(3) fluid nutrient medium preparation: add 3 times of water, 65 ℃ of water-bath saccharification 8 hours after fructus hordei germinatus pulverized, filter, add that water and weak acid are regulated pol and pH value is respectively 15 ripple woodss, 6, under 121 ℃ of temperature, sterilized 20 minutes then;
(4) Liquid Culture: add the bacteria suspension for preparing in being cooled to 28-32 ℃ fluid nutrient medium, 30 ℃ of water-baths are cultivated, and stop when obviously muddy to the fluid nutrient medium of clarification;
(5) solid medium preparation: with wheat bran, corn flour, dregs of beans and urea according to 82: 7: 11.8: 0.2 ratio is mixed, and adds 60% water, evenly mixes back sterilization 20 minutes under 121 ℃ of temperature;
(6) cultivate: cultured fluid nutrient medium is admixed in the sterilized solid medium, and inoculum concentration is 10%, puts into tray and carries out fermented and cultured, 30 ℃ of temperature, time 72h;
(7) drying: low temperature drying after the fermentation ends, packing are made product.
Product of the present invention is to adopt such method to make:
(1) rejuvenation of spawn: use aseptic technique to change in the test tube that sterilized solid medium is housed the test tube CB barms of preserving, under 30 ℃ of constant temperatures, cultivated 3-5 days;
(2) bacteria suspension preparation: inject SPSS in cultivating the test tube that finishes, the injection rate of SPSS is the 1/4-1/3 of test tube volume, and vibration is washed, up to the physiological saline muddiness;
(3) fluid nutrient medium preparation: add 3 times of water, 65 ℃ of water-bath saccharification 8 hours after fructus hordei germinatus pulverized, filter, add that water and weak acid are regulated pol and pH value is respectively 15 ripple woodss, 6, under 121 ℃ of temperature, sterilized 20 minutes then;
(4) Liquid Culture: add the bacteria suspension for preparing in being cooled to 28-32 ℃ fluid nutrient medium, 30 ℃ of water-baths are cultivated, and stop when obviously muddy to the fluid nutrient medium of clarification;
(5) solid medium preparation: with wheat bran, corn flour, dregs of beans and urea according to 82: 7: 11.8: 0.2 ratio is mixed, and adds 60% water, evenly mixes back sterilization 20 minutes under 121 ℃ of temperature;
(6) cultivate: cultured fluid nutrient medium is admixed in the sterilized solid medium, and inoculum concentration is 10%, puts into tray and carries out fermented and cultured, 30 ℃ of temperature, time 72h;
(7) drying: low temperature drying after the fermentation ends, packing are made product.
Preparation method of the present invention can also comprise:
1, use Bulgarian Lactobacillus and phosphoric acid that sweat pH value is regulated and control in the fermented and cultured process, fermentation final pH value is at 5-6.
2, the time of Liquid Culture is 20 hours.
The present invention picks out the improved seeds of under China's present stage raising condition (maize straw+vinasse+fine fodder) by to the screenings of different bacterium, utilizes the discarded object of agricultural production to ferment under certain conditions through specially treated as raw material and makes product.This product mainly is by changing the lumen fermentation type, increase the capacity usage ratio of animal to roughage, finally reaching the purpose of volume increase.Preparation method advanced person of the present invention, the pH value control technique of use compound bacteria.Product has cheap (2 000 yuan/ton), use characteristics such as simple, that addition is few, additive effect is obvious, have no adverse reaction.Through evidence, add 50g product of the present invention, can improve output of milk 2Kg, if the milk price of butterfat percnetage 3.5% is 1.6 yuan/kg, then input-output ratio is 1: 32.Viable count 70~10,000,000,000 of product of the present invention/gram dry.
Product of the present invention bovine lactation early stage (0-140d) that can be used for suckling, also can be used for whole giving milk the phase, can also be used for nonruminant, all have a significant effect, and can competitively regulate intestinal beneficial bacterium quantity, obviously reduce the intestines problem of animal, product is admixed in the fine fodder fed consumption 50g/ head. day.Also can directly sneak in feed, adding quantity is 1~3%.This product stability is better, can keep biologically active six months at normal temperature condition.Viable count can remain on more than 85% under 15 minutes situation of 60 ℃ of oven dry.
Product of the present invention is as green additive, and the color and luster canescence is mixed with certain white powder material, tart flavour is arranged slightly, product is mainly regulated and control cow rumen by dual mode, and the firstth, the living cells that contains in the product, the living cells quantity in this product can reach 6,000,000,000 cfu/g; The secondth, the active factors that contains in the product.By the two acting in conjunction the little ecology of cow rumen is regulated, thereby improve the output of milk.
(4) specific embodiments
For example the present invention is done more detailed description below:
The saccharomycete name of using is called CB yeast (Candida tropicalis Berkhout) bacterial classification, for guaranteeing product quality, adopts the solid-liquid composting fermentation, strengthens the inoculum concentration of liquid culture.The concrete operations step is as follows:
1, rejuvenation of spawn: use aseptic technique to change in the test tube that sterilized solid medium is housed the test tube strains of preserving, at 30 ℃ of constant temperature culture 3-5 days.
2, bacteria suspension preparation: inject SPSS in cultivating the test tube that finishes, be approximately 1/4 of test tube volume, vibration is washed, up to the physiological saline muddiness.
3, fluid nutrient medium preparation: fructus hordei germinatus adds water, water-bath saccharification after pulverizing, and filters, and regulates pol and pH value and is respectively 15 ripple woodss, 6, sterilizes 20 minutes for 121 ℃ then.
4, Liquid Culture: add the bacteria suspension for preparing in the fluid nutrient medium that is cooled to about 30 ℃, 30 ℃ of water-baths are cultivated,, when the fluid nutrient medium of clarifying is obviously muddy, stop, needing 20h.
5, solid medium preparation: is 82: 7: 11.8 with wheat bran, corn flour, dregs of beans and urea according to weight ratio: 0.2 ratio is mixed, and adds 60% water, evenly mixes back 121 ℃ of sterilizations.
6, cultivate: cultured fluid nutrient medium is admixed in the sterilized solid medium, and inoculum concentration is 10%, puts into tray and cultivates, 30 ℃ of temperature, time 72h.
7, drying: low temperature drying after the fermentation ends, packing make product.
This technology also uses Bulgarian Lactobacillus and phosphoric acid that sweat pH value is regulated and control.Guarantee profitable strain quantity, the control varied bacteria growing.Fermentation final pH value about 5.5.
Claims (4)
1, a kind of cow feed additive that improves milk cow production performance is characterized in that: it is that the CB yeast adopts following method to cultivate resulting product:
(1) rejuvenation of spawn: use aseptic technique to change in the test tube that sterilized solid medium is housed the test tube strains of preserving, under 30 ℃ of constant temperatures, cultivated 3-5 days;
(2) bacteria suspension preparation: inject SPSS in cultivating the test tube that finishes, the injection rate of SPSS is the 1/4-1/3 of test tube volume, and vibration is washed, up to the physiological saline muddiness;
(3) fluid nutrient medium preparation: add 3 times of water, 65 ℃ of water-bath saccharification 8 hours after fructus hordei germinatus pulverized, filter, add that water and weak acid are regulated pol and pH value is respectively 15 ripple woodss, 6, under 121 ℃ of temperature, sterilized 20 minutes then;
(4) Liquid Culture: add the bacteria suspension for preparing in being cooled to 28-32 ℃ fluid nutrient medium, 30 ℃ of water-baths are cultivated, and stop when obviously muddy to the fluid nutrient medium of clarification;
(5) solid medium preparation: with wheat bran, corn flour, dregs of beans and urea according to 82: 7: 11.8: 0.2 ratio is mixed, and adds 60% water, evenly mixes back sterilization 20 minutes under 121 ℃ of temperature;
(6) cultivate: cultured fluid nutrient medium is admixed in the sterilized solid medium, and inoculum concentration is 10%, puts into tray and carries out fermented and cultured, 30 ℃ of temperature, time 72h;
(7) drying: low temperature drying after the fermentation ends, packing.
2, a kind of preparation method that improves the cow feed additive of milk cow production performance is characterized in that:
(1) rejuvenation of spawn: use aseptic technique to change in the test tube that sterilized solid medium is housed the test tube CB barms of preserving, under 30 ℃ of constant temperatures, cultivated 3-5 days;
(2) bacteria suspension preparation: inject SPSS in cultivating the test tube that finishes, the injection rate of SPSS is the 1/4-1/3 of test tube volume, and vibration is washed, up to the physiological saline muddiness;
(3) fluid nutrient medium preparation: add 3 times of water, 65 ℃ of water-bath saccharification 8 hours after fructus hordei germinatus pulverized, filter, add that water and weak acid are regulated pol and pH value is respectively 15 ripple woodss, 6, under 121 ℃ of temperature, sterilized 20 minutes then;
(4) Liquid Culture: add the bacteria suspension for preparing in being cooled to 28-32 ℃ fluid nutrient medium, 30 ℃ of water-baths are cultivated, and stop when obviously muddy to the fluid nutrient medium of clarification;
(5) solid medium preparation: with wheat bran, corn flour, dregs of beans and urea according to 82: 7: 11.8: 0.2 ratio is mixed, and adds 60% water, evenly mixes back sterilization 20 minutes under 121 ℃ of temperature;
(6) cultivate: cultured fluid nutrient medium is admixed in the sterilized solid medium, and inoculum concentration is 10%, puts into tray and carries out fermented and cultured, 30 ℃ of temperature, time 72h;
(7) drying: low temperature drying after the fermentation ends, packing are made product.
3, the preparation method of the cow feed additive of raising milk cow production performance according to claim 2 is characterized in that: use Bulgarian Lactobacillus and phosphoric acid that sweat pH value is regulated and control in the fermented and cultured process, fermentation final pH value is at 5-6.
4, according to the preparation method of the cow feed additive of claim 2 or 3 described raising milk cow production performances, it is characterized in that: the time of Liquid Culture is 20 hours.
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CNB2005100099516A CN1319466C (en) | 2005-04-29 | 2005-04-29 | Dairy cattle feed additive for improving milk cow production performance and its manufacturing method |
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Cited By (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102204640A (en) * | 2011-01-13 | 2011-10-05 | 东北农业大学 | Novel concentrated feed for raising milk nutritional value and preparation method thereof |
CN103222563A (en) * | 2013-04-19 | 2013-07-31 | 安徽强农牧业有限公司 | Forage capable of increasing disease resistance of dairy cows, and preparation method thereof |
CN106071144A (en) * | 2016-06-25 | 2016-11-09 | 聂超 | A kind of applicable feed formula that winter, milch cow was fed |
CN117617386A (en) * | 2024-01-26 | 2024-03-01 | 内蒙古农业大学 | Ruminant intestinal tract regulating agent |
-
2005
- 2005-04-29 CN CNB2005100099516A patent/CN1319466C/en not_active Expired - Fee Related
Cited By (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN102204640A (en) * | 2011-01-13 | 2011-10-05 | 东北农业大学 | Novel concentrated feed for raising milk nutritional value and preparation method thereof |
CN102204640B (en) * | 2011-01-13 | 2012-07-25 | 东北农业大学 | Novel concentrated feed for raising milk nutritional value and preparation method thereof |
CN103222563A (en) * | 2013-04-19 | 2013-07-31 | 安徽强农牧业有限公司 | Forage capable of increasing disease resistance of dairy cows, and preparation method thereof |
CN106071144A (en) * | 2016-06-25 | 2016-11-09 | 聂超 | A kind of applicable feed formula that winter, milch cow was fed |
CN117617386A (en) * | 2024-01-26 | 2024-03-01 | 内蒙古农业大学 | Ruminant intestinal tract regulating agent |
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CN1319466C (en) | 2007-06-06 |
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