CN1597984A - 一种sars冠状病毒诊断基因芯片 - Google Patents
一种sars冠状病毒诊断基因芯片 Download PDFInfo
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- CN1597984A CN1597984A CNA2004100220900A CN200410022090A CN1597984A CN 1597984 A CN1597984 A CN 1597984A CN A2004100220900 A CNA2004100220900 A CN A2004100220900A CN 200410022090 A CN200410022090 A CN 200410022090A CN 1597984 A CN1597984 A CN 1597984A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
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Abstract
本发明涉及一种SARS冠状病毒诊断基因芯片,属于生物技术领域。本发明的芯片用确定的12个SARS冠状病毒特异的cDNA片段作为检测位点,利用12对PCR引物制备探针DNA,另外利用位于探针位点外侧序列的12对PCR引物作为检测引物,通过PCR扩增待检测样本cDNA,标记后与芯片杂交,根据杂交信号判断样本中是否有SARS冠状病毒。该芯片所用探针制备引物和检测引物也可单独用于PCR检测引物;该芯片也可以用于食品,环境以及检疫中的SARS冠状病毒的检测。本发明具有以下优点:1.减少因个体差异引起的漏检2.检测灵敏度和特异性高;3.消除系统误差和控制检测系统污染等。该技术还可用于食品,环境以及检疫中的SARS冠状病毒的检测。
Description
技术领域:
本发明涉及一种SARS冠状病毒诊断基因芯片,属于生物技术领域。
背景技术:
非典型性肺炎即严重呼吸道感染综合症(SARS)是一种传染性非常强的传染性疾病,2003年2-5月的短短3个多月,在全世界范围内广泛流行,造成近8000人感染发病,造成600多人死亡,严重威胁人类健康和生命安全,引起世界卫生组织和各国政府的高度重视。
有效的检测相关病原物是有效控制该疾病流行的基础。由于该病病原物是新的病原物,流行之初无法检测,只能根据临床症状进行判断,导致很多病毒携带者到处流动而传染给其他人。同时有的病人症状与“非典”相似,也被怀疑是“非典”而与真正的“非典”隔离在一起而感染上“非典”。随后应急产生的一些免疫诊断方法和PCR方法都因技术本身的限制,致使检测准确性低。因此一种有效的检测方法的建立显得特别的重要。基因芯片技术是一种新兴的生物技术,它在生物学各领域有广泛的应用前景,临床诊断是其应用领域之一,利用基因芯片诊断SARS病毒可以弥补现有诊断方法的不足。
SARS病毒的病原物经过世界几个国家的科学家的努力,在2个月左右的时间内测定了其核酸序列,发现全长序列29.4Kb,与现有冠状病毒的核酸序列同源性较小(<10%)。根据已经公布的核酸序列,研制诊断基因芯片成为可能。
发明内容:
本发明的.芯片用12个SARS冠状病毒特异的cDNA片段作为探针,并设计了芯片探针制造的PCR引物和和芯片使用所用的检测引物序列(详见序列表),按现有技术(本发明人申请的,申请号021280681、02128069X)制造基因芯片和检测应用。该芯片所用探针制备引物和检测引物也可单独用于PCR检测引物;该芯片也可以用于食品,环境以及检疫中的SARS冠状病毒的检测。
本发明的优点:
1.基因芯片诊断由于多位点可以有效地减少因个体差异引起的漏检。
2.本产品的检测灵敏度较PCR方法的检测限降低2-3个数量级,可达102-103/ml病毒分子。
3.由于有足够的容量空间,可以设置各种对照,消除系统误差和控制检测系统污染。
4.检测过程中,通过聚乙二醇沉淀,使病毒得以浓缩,提高检测灵敏度。
附图说明:
附图为点阵图。图中I、II、III、IV为阳性对照点,其余编号为检测位点号。
附件包含芯片上的12个检测位点的检测序列(共12条)、探针引物(共24条)和检测引物(共24条)。使用时位点X(X表示1、2、3.....12)检测序列,用位点X探针引物制备探针,用位点X检测引物进行检测。
具体实施方式:
本发明的实施按如下程序进行:
1.利用internet网络搜索SARS冠状病毒核酸序列,并利用NIH网站上的在线工具检出SARS冠状病毒基因组中不同于其它冠状病毒的核酸序列,根据获得的SARS病毒特异序列,设计了12个检测位点,通过扩增标准SARS冠状病毒cDNA获得cDNA片段;
2.克隆PCR扩增的SARS冠状病毒cDNA片段,测序确定克隆的正确性。共获得12个序列正确的克隆,用PCR扩增相应的片段作为芯片制造的探针DNA;
3.按如下工艺制造芯片:
1).用探针制备引物(引物序列见附件probe make primer)进行PCR扩增,克隆得SARS特异cDNA,其产物用乙醇沉淀,1XTE溶解,并用电泳方法定量;
2).定量的PCR产物用3×SSC稀释至0.3ug/ul;
3).用点样仪点样在用L-多聚赖氨酸包被处理的载玻片上,点阵图见附图,用紫外交联法固定DNA在载玻片上。
4.使用时,采用现有的方法(见专利申请号:021280681,02128069X),但由于不同的用途,取样方法和制备方法也不同。
(1)取样:
1)临床样品:用灭菌的生理盐水约5ml给病人漱口,漱口时仰着脖子,洗上呼吸道约10秒钟,吐出漱口水用50ml离心管收集漱口水;
2)蔬菜及水果:用生理盐水约5ml洗待检测样品的表面,洗下的溶液收集于50ml的离心管中;
3)其他食物,用约5ml生理盐水与待检样品约1-2克,一起搅拌。10000g离心,取上清液于50ml离心管中。
(2).样品处理:上述含病毒的溶液加等体积的1.6mol/L的生理盐水配置的13%聚乙二醇8000,混匀,10000g离心10分钟,去上清,沉淀加入100ul生理盐水悬浮沉淀物;
(3)用常规的Trizol试剂分离RNA,并用常规的一步RT-PCR法进行PCR扩增,检测引物见附件(detection primer)。
(4)按现有的芯片检测专利021280681,02128069X方法检测核酸的存在性。
5.按上述检测程序,对标准样本进行测试100%检测可靠。
Organization Applicant
-----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
--------------------
<120>Title:位点1检测序列
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
gccatcattt tggcatcttt ctctgcttct acaagtgcct ttattgacac tataaagagt 60
cttgattaca agtctttcaa aaccattgtt gagtcctgcg gtaactataa agttaccaag 120
ggaaagcccg taaaaggtgc ttggaacatt ggacaacaga gatcagtttt aacaccactg 180
tgtggttttc cctcacaggc tgctggtgtt atcagatcaa tttttgcgcg cacacttgat 240
gcagcaaacc 250
<212>Type:DNA
<211>Length:250
SequenceName:sequence 1
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点1探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cattgttgag tcctgcggta 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 25
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点1探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
taacaccagc agcctgtgag 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 26
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点1检测上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
gccatcattt tggcatcttt 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 1
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点1检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
ggtttgctgc atcaagtgtg 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 2
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点2检测序列
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
gcaaagttgc gatcactcaa cttaggtgaa gtcttcatcg ctcaaagcaa gggactttac 60
cgtcagtgta tacgtggcaa ggagcagctg caactactca tgcctcttaa ggcaccaaaa 120
gaagtaacct ttcttgaagg tgattcacat gacacagtac ttacctctga ggaggttgtt 180
ctcaagaacg gtgaactcga agcactcgag acgcccgttg atagcttcac aaatggagct 240
atcgttggca c 251
<212>Type:DNA
<211>Length:251
SequenceName:sequence 2
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>Organizat ionName:云南大学
Application Project
-------------------
<120>Title:位点2探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
tgtatacgtg gcaaggagca 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 27
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点2探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
tctcgagtgc ttcgagttca 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 28
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点2检测上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
gcaaagttgc gatcactcaa 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 3
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点2检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<14t>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
gtgccaacga tagctccatt 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 4
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>Organizat ionName:云南大学
Application Project
-------------------
<120>Title:位点3检测序列
<t30>AppFi leReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
taccctccaa aaaggctggt ggcactactg agatgctctc aagagctttg aagaaagtgc 60
cagttgatga gtatataacc acgtaccctg gacaaggatg tgctggttat acacttgagg 120
aagctaagac tgctcttaag aaatgcaaat ctgcatttta tgtactacct tcagaagcac 180
ctaatgctaa ggaagagatt ctaggaactg tatcctggaa tttgagagaa atgcttgctc 240
atgctga 247
<212>Type:DNA
<211>Length:247
SequenceName:sequence 3
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点3探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
aaccacgtac cctggacaag 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 29
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点3探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
agcattaggt gcttctgaag gt 22
<212>Type:DNA
<211>Length:22
SequenceName:primer 30
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点3检测上游引物
<130>AppFi leReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
taccctccaa aaaggctggt 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 5
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点3检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
tcagcatgag caagcatttc 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 6
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点4检测序列
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
tgctcgtgat ttgtcactcc agtttaaaag accaatcaac cctactgacc agtcatcgta 60
tattgttgat agtgttgctg tgaaaaatgg cgcgcttcac ctctactttg acaaggctgg 120
tcaaaagacc tatgagagac atccgctctc ccattttgtc aatttagaca atttgagagc 180
taacaacact aaaggttcac tgcctattaa tgtcatagtt tttgatggca agtccaaatg 240
cgacgagtct 250
<212>Type:DNA
<211>Length:250
SequenceName:sequence 4
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点4探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
ccaatcaacc ctactgacca 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 31
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
--------------------
<120>Title:位点4探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
ggagagcgga tgtctctcat 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 32
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点4检测上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
tgctcgtgat ttgtcactcc 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 7
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点4检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
agactcgtcg catttggact 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 8
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点5检测序列
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
tggttttgac gcatggttta gccagcgtgg tggttcatac aaaaatgaca aaagctgccc 60
tgtagtagct gctatcatta caagagagat tggtttcata gtgcctggct taccgggtac 120
tgtgctgaga gcaatcaatg gtgacttctt gcattttcta cctcgtgttt ttagtgctgt 180
tggcaacatt tgctacacac cttccaaact cattgagtat agtgattttg ctacctctgc 240
ttgcgttct 249
<212>Type:DNA
<211>Length:249
SequenceName:sequence 5
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点5探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
aatgacaaaa gctgccctgt 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 33
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点5探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
ttgccaacag cactaaaaac a 21
<212>Type:DNA
<211>Length:21
SequenceName:primer 34
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点5检测上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
tggttttgac gcatggttta 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 9
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点5检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
agaacgcaag cagaggtagc 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 10
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点6检测序列
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
ggctgcaggt acagacacaa ccataacatt aaatgttttg gcatggctgt atgctgctgt 60
tatcaatggt gataggtggt ttcttaatag attcaccact actttgaatg actttaacct 120
tgtggcaatg aagtacaact atgaaccttt gacacaagat catgttgaca tattgggacc 180
tctttctgct caaacnggaa ttgccgtctt agatatgtgt gctgctttga aagagctgct 240
gcagaatggt 250
<212>Type:DNA
<211>Length:250
SequenceName:sequence 6
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点6探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
catggctgta tgctgctgtt 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 35
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点6探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
gagcagaaag aggtcccaat 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 36
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点6检测上游引物
<130>AppFi leReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
ggctgcaggt acagacacaa 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 11
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点6检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
accattctgc agcagctctt 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 12
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点7检测序列
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
ttgttgatgc ggatagcaag attgttcaac ttagtgaaat taacatggac aattcaccaa 60
atttggcttg gcctcttatt gttacagctc taagagccaa ctcagctgtt aaactacaga 120
ataatgaact gagtccagta gcactacgac agatgtcctg tgcggctggt accacacaaa 180
cagcttgtac tgatgacaat gcacttgcct actataacaa ttcgaaggga ggtaggtttg 240
tgctggcatt 250
<212>Type:DNA
<211>Length:250
SequenceName:sequence 7
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点7探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
tttggcttgg cctcttattg 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 37
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点7探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
aggcaagtgc attgtcatca 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 38
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点7检测上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
ttgttgatgc ggatagcaag 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 13
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点7检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
aatgccagca caaacctacc 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 14
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点8检测序列
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cccaggttgt gatgtcactg atgtgacaca actgtatcta ggaggtatga gctattattg 60
caagtcacat aagcctccca ttagttttcc attatgtgct aatggtcagg tttttggttt 120
atacaaaaac acatgtgtag gcagtgacaa tgtcactgac ttcaatgcga tagcaacatg 180
tgattggact aatgctggcg attacatact tgccaacact tgtactgaga gactcaagct 240
tttcgcagc 249
<212>Type:DNA
<211>Length:249
SequenceName:sequence 8
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
Emai lAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点8探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
aagcctccca ttagttttcc a 21
<212>Type:DNA
<211>Length:21
SequenceName:primer 39
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点8探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
aatcgccagc attagtccaa 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 40
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点8检测上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cccaggttgt gatgtcactg 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 15
SequenceDescription:
Organization Applicant
-----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点8检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
gctgcgaaaa gcttgagtct 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 16
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点9检测序列
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cagacaatgg ttgccaactg gcacactaac ttgtcgattc agatcttaat gacttcgtct 60
ccgacgcaga ttctacttta attggagact gtgcaacagt acatacggct aataaatggg 120
accttattat tagcgatatg tatgacccta ggaccaaaca tgtgacaaaa gagaatgact 180
ctaaagaagg gtttttcact tatctgtgtg gatttataaa gcaaaaacta gccctgggtg 240
gtt 243
<212>Type:DNA
<211>Length:243
SequenceName:sequence 9
SequenceDescfiption:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:dytan@ynu.edu.cn
EmailAddress:
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点9探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cgtctccgac gcagattcta 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 41
SequenceDescription:
Organization Applicant
-----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点9探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cttttgtcac atgtttggtc ct 22
<212>Type:DNA
<211>Length:22
SequenceName:primer 42
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@vnu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点9检测上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cagacaatgg ttgccaactg 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 17
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点9检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
aaccacccag ggctagtttt 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 18
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点10检测序列
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cttgctcttt tgggggtgta agtgtaatta cacctggaac aaatgcttca tctgaagttg 60
ctgttctata tcaagatgtt aactgcactg atgtttctac agcaattcat gcagatcaac 120
tcacaccagc ttggcgcata tattctactg gaaacaatgt attccagact caagcaggct 180
gtcttatagg agctgagcat gtcgacactt cttatgagtg cgacattcct attggagctg 240
gcatttgtg 249
<212>Type:DNA
<211>Length:249
SequenceName:sequence 10
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点10探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cacctggaac aaatgcttca t 21
<212>Type:DNA
<211>Length:21
SequenceName:primer 43
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点10探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
agcctgcttg agtctggaat 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 44
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点10检测上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cttgctcttt tgggggtgta 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 19
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点10检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cacaaatgcc agctccaata 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 20
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点11检测序列
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
accgctcatg gaaagtgaac ttgtcattgg tgctgtgatc attcgtggtc acttgcgaat 60
ggccggacac tccctagggc gctgtgacat taaggacctg ccaaaagaga tcactgtggc 120
tacatcacga acgctttctt attacaaatt aggagcgtcg cagcgtgtag gcactgattc 180
aggttttgct gcatacaacc gctaccgtat tggaaactat aaattaaata cagaccacgc 240
cggtag 246
<212>Type:DNA
<211>Length:246
SequenceName:sequence 11
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点11探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
ttggtgctgt gatcattcgt 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 45
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点11探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
cctgaatcag tgcctacacg 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 46
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点11检测上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
accgctcatg gaaagtgaac 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 21
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
--------------------
<120>Title:位点11检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
ctaccggcgt ggtctgtatt 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 22
SequenceDescription:
Organization Applicant
------------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点12检测序列
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
tcagccccag atggtacttc tattacctag gaactggccc agaagcttca cttccctacg 60
gcgctnacaa agaaggcatc gtatgggttg caactgaggg agccttgaat acacccaaag 120
accacattgg cacccgcaat cctaataaca atgctgccac cgtgctacaa cttcctcaag 180
gaacaacatt gcctaaaagg cttctacgca gagggaagca gaggcggcag tcaagcctct 240
tctcgct 247
<212>Type:DNA
<211>Length:247
SequenceName:sequence 12
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点12探针上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
agaaggcatc gtatgggttg 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 47
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点12探针下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
ccctctgcgt agaagccttt 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 48
SequenceDescription:
Organization Applicant
-------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>OrganizationName:云南大学
Application Project
-------------------
<120>Title:位点12检测上游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
tcagccccag atggtacttc 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 23
SequenceDescription:
Organization Applicant
----------------------
Street:翠湖北路2号
City:昆明市
State:云南省
Country:中国
PostalCode:650091
PhoneNumber:0871-5032061
FaxNumber:
EmailAddress:dytan@ynu.edu.cn
<110>Organizat ionName:云南大学
Application Project
--------------------
<120>Title:位点12检测下游引物
<130>AppFileReference:一种SARS冠状病毒诊断基因芯片
<140>CurrentAppNumber:
<141>CurrentFilingDate:2004-03-10
Sequence
--------
<213>OrganismName:virus
<400>PreSequenceString:
agcgagaaga ggcttgactg 20
<212>Type:DNA
<211>Length:20
SequenceName:primer 24
SequenceDescription:
Claims (1)
1、一种SARS冠状病毒诊断基因芯片,其特征在于该.芯片确定了12个SARS冠状病毒特异的cDNA片段作为检测位点,利用12对PCR引物制备探针DNA,另外利用位于探针位点外侧序列的12对PCR引物作为检测引物,通过PCR扩增待检测样本cDNA,标记后与芯片杂交,根据杂交信号判断样本中是否有SARS冠状病毒。
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2004100220900A CN1285736C (zh) | 2004-03-18 | 2004-03-18 | 一种sars冠状病毒诊断基因芯片 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CNB2004100220900A CN1285736C (zh) | 2004-03-18 | 2004-03-18 | 一种sars冠状病毒诊断基因芯片 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1597984A true CN1597984A (zh) | 2005-03-23 |
CN1285736C CN1285736C (zh) | 2006-11-22 |
Family
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CNB2004100220900A Expired - Fee Related CN1285736C (zh) | 2004-03-18 | 2004-03-18 | 一种sars冠状病毒诊断基因芯片 |
Country Status (1)
Country | Link |
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CN (1) | CN1285736C (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111808989A (zh) * | 2020-06-18 | 2020-10-23 | 重庆浦洛通基因医学研究院有限公司 | 一种冠状病毒/流感病毒/鼻病毒核酸联合检测试剂盒及使用方法 |
-
2004
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111808989A (zh) * | 2020-06-18 | 2020-10-23 | 重庆浦洛通基因医学研究院有限公司 | 一种冠状病毒/流感病毒/鼻病毒核酸联合检测试剂盒及使用方法 |
Also Published As
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CN1285736C (zh) | 2006-11-22 |
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