CN1559506A - Anti-parasite for eel - Google Patents
Anti-parasite for eel Download PDFInfo
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- CN1559506A CN1559506A CNA2004100128088A CN200410012808A CN1559506A CN 1559506 A CN1559506 A CN 1559506A CN A2004100128088 A CNA2004100128088 A CN A2004100128088A CN 200410012808 A CN200410012808 A CN 200410012808A CN 1559506 A CN1559506 A CN 1559506A
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- anthelmintic
- monopteri albi
- fructus quisqualis
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Abstract
An antiparasite for eel is prepared from 4 Chinese-medicinal materials including quisqualis fruit, cyrtomian rhizome, stemona root, etc.
Description
Technical field
The present invention relates to the disease control category of special aquaculture, relate in particular to a kind of Monopteri albi anthelmintic that is exclusively used in control Monopteri albi intestinal and endoparasite.
Background technology
Monopterus albus (Zuiew) (
Monopterus albus) be the important warm water economic fish of China, edible since ancient times Monopterus albus (Zuiew) integration of edible and medicinal herbs, also polyphagia with emaciation is mended, and " Monopterus albus (Zuiew) is sweet in flavor and warm in property, nontoxicly goes into virtuous warp in the Compendium of Material Medica record.Can tonifying deficiency, bone and muscle strengthening, curing rheumatism, can treat diseases such as consumptive disease wound, rheumatism splenalgia, dysentery pus and blood, nodule in the breast.Its weak and incompetent skin, flesh and blood all can be used as medicine.”
The Monopterus albus (Zuiew) edible part is up to 65%.Delicious flavour is nutritious, and except that rich in proteins, its calcium iron content occupies first of the fresh-water fishes.Therefore sell well in domestic and international market, be one of Fish principal item of foreign exchange earning always.
Along with the minimizing of Monopterus albus (Zuiew) natural resources and the increase of market demand, being broken through gradually of the scale intensive culture technology of Monopterus albus (Zuiew), people consume custom, and also to trend towards fresh aquatic food day by day be main.In breeding scale, Monopterus albus (Zuiew) disease incidence rate increases thereupon, has directly restricted intensification, scale, the commercialization development of Monopterus albus (Zuiew).Produce the raiser simultaneously in order to solve the disease problem, use various chemical agents.Wherein also some is to belong to the poisonous and harmful medicine that China expressly bans use of.The abuse of medicine very easily causes breeding water body to pollute, poisonous and harmful substance and medicine is residual increases in the Monopterus albus (Zuiew) body.Edible safety for production environment, technology input and the product of Aquatic product in China implements since calendar year 2001 " nuisanceless action plan " has carried out the series of standards regulation, " aquatic product quality detect in lay special stress on to the detectability of multiple drug residue poisonous and harmful substance; limiting the quantity of so that fisheries drug is residual in People's Republic of China's agricultural industry criteria " NY5070-2002 pollution-free food " aquatic products; and " NY5168-2002 pollution-free food Monopterus albus (Zuiew) ", is exactly the domestic standard that aquatic products must be carried out.Strictly stipulated in the aquaculture, cultured the forbidden drugs scope of application of object.The disease control of culture of swamp eel, medicine safe in utilization are the important techniques key points.
Current Monopteri albi anthelmintic on the market has tens of kinds more than, " five worms are clean " as the production of Jiangxi fish sun, and the fish medicine that other factories produce has: the pleasure of stomach, the Chinese yellow eel worm is clean, rich No. 2 of fish, the Monopteri albi Shachongwang (pesticide), sharp stomach looses, the anthelmintic gunman, dimehypo, dichlorvos, Bayer 71628 etc., these medicines or with organophosphorus insecticide remodeling preparation, with chemical anthelmintic is the Main Ingredients and Appearance preparation, how many above fish medicines exists certain disadvantage, some is the pollution or the extended residual fish body that can be able to cause water body environment, some ingredients is dull can to influence ingesting of Monopteri albi, unfavorable its growth.
Summary of the invention
The objective of the invention is in order to solve the deficiency that the above-mentioned background technology exists, propose a kind of evident in efficacyly, residual few, be difficult for developing immunity to drugs, the Monopteri albi anthelmintic that can not pollute the aquatic ecological environment.
For achieving the above object, the present invention adopts following technical scheme: a kind of Monopteri albi anthelmintic is characterized in that the medicament that this medicine adopts following weight proportion material medicine to make: Fructus Quisqualis 30-50%, Rhizoma Osmundae 10-30%, Radix Stemonae 10-30%, Semen Torreyae 10-30%.
Above-mentioned medicament adopts powder to be advisable,
Fructus Quisqualis (
Quisqualis indica L), another name stays asks son, five ribs, comet fruit.Seed for the Combretum Racemosum plant.Chemical constituent contains Fructus Quisqualis acid potassium, oleic acid Palmic acid, stearic acid, linoleic acid, myristic acid, sterol etc.The sweet temperature of the property of medicine is returned the taste warp.Effect with parasite killing, removing food stagnancy, spleen invigorating.Ascarid, pinworm, Lumbricus, Hirudo are had stronger anesthesia drive away usefulness, traditional Chinese medical science research, its Fructus Quisqualis potassium acid is the anthelmintic effective ingredient.
The Radix Stemonae [
Stemoua sessilisolia (miq)], be the tuber of Radix Stemonae section plant.Chemical constituent mainly contain alkaloid several, (-)-Stemoninine, Stemonidine, different (-)-Stemoninine, hordorine etc., and glycolipid class, protein, formic acid, acetic acid, oxalic acid etc.Property of medicine hardship, sweet, tepor are returned lung meridian, have resisting pathogenic microbes effect and insecticidal action, and pinworm etc. is had expulsion and killing action.Its alkaloids extract of pharmacological research causes the guinea pig bronchial smooth muscle spasm to histamine slow and persistent relexation.
Rhizoma Osmundae (
Dryopteris Crassirhizoma Nalai), phosphorus hair kind is slightly through phosphorus hair Herba pteridii latiusculi rhizome.Main chemical compositions contains phloroglucinol derivant pannol, Filixic Acids, flavaspidic acid, also contains triterpenoid adianton, gentle matter, volatile oil resin etc.Property of medicine hardship is slightly cold and returns the liver spleen channel.Have anthelmintic, antibiosis and antiviral functions.Ascaris suum, Lumbricus, Hirudo are killed and the expeling effect, and cattle fasciola and wealthy dish worm are had therapeutic efficiency.Its anthelmintic pharmacology is pannol, the Filixic Acids toxic action to worm.
Semen Torreyae (
Torreya grandis Fort), taxaceae, the seed of Chinese torreya.Real, the Chinese torreya nut of another name Chinese torreya.Main chemical contains linoleic acid, stearic acid, oleic acid, sterol, oxalic acid, lauric acid, myristic acid, Palmic acid, glucose, polysaccharide etc.The property of medicine is sweet flat, returns lung, large intestine channel.To multiple entero-vioform expulsion, insecticidal action, there is removing food stagnancy to moisturize effect.It all has the inhibition killing effect to ancylostome, cestode, pinworm pharmacological research.Its multiple fat lubricates intestinal and is beneficial to the polypide discharge.
More than four kinds of material medicines become powder or water preparation medicine by the aforementioned ratio compatibility, it is admixed in the fish meal uses, the intravital various parasites of Monopteri albi are all had the expeling effect.
The present invention also can add levamisole (chemosynthesis anthelmintic) and adhesive material as adjuvant adopting on above-mentioned four kinds of material medicine bases, to quicken the curative effect of medicament, reach the anthelmintic purpose of quick parasite killing, also make it have stronger adhesiveness simultaneously, be suitable for mixing raising and throw something and feed.By this scheme, the optimum weight percentage ratio of material medicine proportioning of the present invention is Fructus Quisqualis 30-50%, Rhizoma Osmundae 10-30%, Radix Stemonae 10-30%, Semen Torreyae 10-30%, levamisole 5-10%, adhesive material 5-8%, wherein, Fructus Quisqualis, Rhizoma Osmundae, the Radix Stemonae and four kinds of crude drug dryings of Semen Torreyae, pulverizing get final product with levamisole and adhesive material mix homogeneously behind 60 mesh sieves excessively.
The present invention adopts safe medicinal plants main body, adjuvant in addition, and the special-purpose anthelmintic medicament of preparation Monopteri albi has the expeling effect to various parasites in the Monopteri albi body.Relatively more existing single anthelmintic prescription medicine has insect-repellent widely.It is obvious that the present invention has curative effect, less residual or do not have, and is difficult for developing immunity to drugs the advantage that can not pollute the aquatic ecological environment.This medicament has stronger adhesiveness, is suitable for mixing raising throwing something and feeding.Mix the bait administration for a long time with this medicine, Monopteri albi does not have medicine source sexually transmitted disease (STD) reason and changes.This medical instrument has attractant, has Wen Xiang to look for food to the poor Monopteri albi of taking food, orectic effect.Through testing the more not parasite recall rate living low about 80% of medication of Monopteri albi medication anthelmintic.Reducing 20%-70% by the incidence rate of culturing the Monopteri albi parasitic disease on probation in the eel cultivation district does not wait.The present invention is ideal Monopterus albus (Zuiew) intestinal anthelmintic medicament, also is subjected to the favorable comment at culture of swamp eel family in practice, and effect is obvious.
Laboratory report
1. materials and methods
1.1 material
1.1.1 Monopterus albus (Zuiew) (
Monopterus albus).Test is chosen in Wuhan City prunus mume (sieb.) sieb.et zucc. man scenery with hills and waters with Monopterus albus (Zuiew) and is produced the wholesale market, selects build normal, does not have deformity, and the body back of the body all mostly is yellowish-brown or cinerous, the abdominal part canescence, and whole body is furnished with many irregular black splotches.Body surface smooth also is rich in hyalomucoid.No wound focus, the energy that is quick on the draw is strong, and body weight is individual uniformly uses fish as test.
1.1.2 medicament stock
The Chinese medicine medical material is available from Wuhan Tai Long Pharma Inc..Raw material stoving was pulverized 60 mesh sieves.
1.1.3 adjuvant
Application has the viscosity raw material that has no side effect, and increases the absorbability of medicine, and messenger drug adheres in the feedstuff, and is not defeated and dispersed, is suitable for having in the water and throws something and feeds.
1.1.4 ancillary drug reaches the anthelmintic purpose of quick parasite killing to quicken the curative effect of medicine.
1.2 method
1.2.1 Chinese medicine preparation test
This test is selected and is used for anthelmintic four kinds of medicines.By its effect, with certain proportion compatibility patent medicine.Medicament is Main Ingredients and Appearance with the Fructus Quisqualis, the sweet temperature of its property of medicine.After curing pulverizing, give out fragrance in the medicine, Monopterus albus (Zuiew) is had the food calling effect, have digestion-promoting spleen-invigorating, anthelmintic multi-efficiency.On prescription,, form anthelmintic medicament to Monopteri albi in conjunction with other three flavors medicines based on this medicine.Multifactor prescription adopts normal experimental design.Every kind of medicine of the proportioning of each medicine of distinguishing the flavor of is got three different proportion water preparations, by orthogonal table L
9(3
4) be arranged with 9 test group.
Table (one) is drafted the ratio % of various medicines
| Level | Fructus Quisqualis | The Radix Stemonae | Rhizoma Osmundae | Semen Torreyae |
| 1 | ????30 | ????20 | ????10 | ????20 |
| 2 | ????40 | ????15 | ????15 | ????15 |
| 3 | ????50 | ????20 | ????10 | ????10 |
Orthogonal table according to four factors, three levels is listed.Each test group such as following table (two)
The design of table (two) orthogonal test gauge outfit
| Row number | 1 | ?2 | ?3 | ?4 |
| Factor | Fructus Quisqualis | The Radix Stemonae | Rhizoma Osmundae | Semen Torreyae |
Press L
9(3
4) each test group of table arrangement back.For the material fish 10 tails Monopterus albus (Zuiew) of throwing something and feeding.Ratio in 2% is added in the ricefield el fodder, and the week back dissection of throwing something and feeding continuously 3 days is tried Monopteri albi.Judge the curative effect of respectively organizing medicine with the Enterozoa recall rate.
1.2.2 tried Monopterus albus (Zuiew) body weight 30-50g, make the 1-90 sequence arrangement by the body weight size, use the random digit Table II, get the 26th horizontally-arranged first number at random and get 90 random digit correspondences each random number downwards as Monopterus albus (Zuiew) sample 1-90 for the starting point horizontally-arranged, by the completely random method 90 tail Monopterus albus (Zuiew)s are divided into 9 groups, the 1-9 group is carried out medicine-feeding test.
1.2.3 fishing with medicament work flow
Former medicine → drying (105 ℃ of electric heating drying to moisture content are lower than 5%) → pulverizing (hammer crusher) → sieve (60 order steel sieve sieve) → encapsulate by proportioning auxiliary material → mixing (blender the mixing) → subpackage of weighing → adds.
1.3.4 test is carried out at 60 * 30 * 30cm glass aquarium
Every group of Monopteri albi put an aquarium respectively, and the depth of water keeps about 10cm.Natural temperature (summer, indoor water temperature was generally at 25-28 ℃) is put into Monopteri albi and was stopped eating one to three day, earlier induces its feed with the Monopteri albi mixed feed, treats that whole Monopteri albis all after the opening food, formally offer medicine and enter test.9 groups of tests of 9 correspondences of investigational agent different proportion prescription fish, 2% of per diem feeding amount is sneaked into feedstuff, and dispensing changes hello no medicine feedstuff then for three days on end, gets Monopteri albi after the week and dissects, and checks Enterozoa and numeration.One group of blank group of only throwing feedstuff is established in this test in addition except that 9 groups of medication tested fish.
2. result and analysis
2.1 result
After 10 days, through dissecting one by one, each test group parasite detects and the results are shown in following table (three) from the dispensing beginning in this test.
Table (three) Enterozoa recall rate
| ????2 | ????0 | ????+2 | ????0 | ????0 | ????0 | ????+2 | ????0 | ????+1 | ??+1 | ??0 | ????+6 | ????40 |
| ????3 | ????0 | ????+2 | ????+3 | ????+4 | ????0 | ????0 | ????+2 | ????0 | ??+1 | ??0 | ????+11 | ????50 |
| ????4 | ????0 | ????0 | ????0 | ????0 | ????+2 | ????0 | ????0 | ????0 | ??+2 | ??0 | ????+4 | ????20 |
| ????5 | ????0 | ????0 | ????0 | ????+3 | ????0 | ????0 | ????+3 | ????0 | ??0 | ??0 | ????6 | ????20 |
| ????6 | ????0 | ????0 | ????0 | ????0 | ????+3 | ????0 | ????0 | ????0 | ??0 | ??0 | ????3 | ????10 |
| ????7 | ????0 | ????0 | ????0 | ????+2 | ????+2 | ????0 | ????0 | ????+3 | ??0 | ??0 | ????7 | ????30 |
| ????8 | ????+2 | ????0 | ????0 | ????0 | ????0 | ????+4 | ????0 | ????0 | ??0 | ??0 | ????6 | ????20 |
| ????9 | ????0 | ????0 | ????0 | ????0 | ????0 | ????0 | ????+3 | ????0 | ??0 | ??+2 | ????5 | ????20 |
| Blank | ????0 | ????+4 | ????+5 | ????+2 | ????+6 | ????+3 | ????+3 | ????0 | ??+4 | ??+4 | ????32 | ????80 |
All borer populations that obviously descended by examination group parasite recall rate and detect are less, and blank group has 80% to detect parasite, and borer population is more relatively.
2.2 analyze
Corresponding to adopting L
9(3
4) 9 groups of pharmaceutical formulation effects of orthogonal table, data analysis such as following table (four).
Table (four) orthogonal test is divided watchman's clapper
| ????7 | ????③50 | ????①20 | ????③10 | ????②15 | ????30 |
| ????8 | ????③50 | ????②15 | ????①20 | ????③10 | ????20 |
| ????9 | ????③50 | ????③10 | ????②15 | ????①20 | ????20 |
| ????k 1 | ????43.3 | ????30 | ????23.3 | ????26.7 | |
| ????k 2 | ????16.7 | ????26.7 | ????26.7 | ????26.7 | |
| ????k 3 | ????23.3 | ????26.7 | ????33.3 | ????30 | |
| ????R | ????26.6 | ????3.3 | ????10 | ????3.3 |
Intuitive analysis is the size of extreme difference value relatively, the extreme difference value 26.6 of Fructus Quisqualis, and the extreme difference value 10 of Rhizoma Osmundae, the Radix Stemonae, Semen Torreyae are all 3.3.Be arranged as Fructus Quisqualis → Rhizoma Osmundae → Radix Stemonae, Semen Torreyae thereby the efficacy effect of finding out in the prescription 4 flavor medicines is descending.During prescription with Fructus Quisqualis as main component, and Rhizoma Osmundae, the Radix Stemonae, Semen Torreyae are considered as auxiliary element.The Fructus Quisqualis price is analyzed from economic angle than higher in the four Chinese medicine, is used as medicine its effect it makes no odds with 50% or 40% ratio.Therefore, it is 40% better to become in the medicine composition Fructus Quisqualis to adopt.The proportioning of other three flavors medicine can be grasped flexibly in the 10-20% scope relatively and adjust.The parasite recall rate that is subjected to the parasite recall rate of examination group and blank group from 9 of table three relatively, medicine is significantly to parasitic expeling effect, the 6th group detects minimum only 10%, the highest by 50%.Parasitic detecting than what go out without medicine inspection the highlyest reduced 87.5% after the medication, minimumly also reduced 37.5%.Therefore, the prescription of the above four Chinese medicine better anthelminthic effect that made up a prescription.
The specific embodiment
Embodiment 1:
The material medicine weight proportion of producing 100 kilograms of Monopteri albi anthelmintics is: Fructus Quisqualis 30kg, Rhizoma Osmundae 30kg, Radix Stemonae 30kg, Semen Torreyae 10kg.
Production technology is: above-mentioned raw materials medicine → drying (105 ℃ of electric heating drying to moisture content are lower than 5%) → pulverizing (hammer crusher) → sieve (60 order steel sieve sieves) → in proportion mixing (blender mixing) → subpackage encapsulation.
During use it is admixed in the fish meal and get final product.
Embodiment 2:
The material medicine weight proportion of producing 100 kilograms of Monopteri albi anthelmintics is: Fructus Quisqualis 40kg, Rhizoma Osmundae 20kg, Radix Stemonae 20kg, Semen Torreyae 20kg.
Production technology is identical with embodiment 1 with using method.
Embodiment 3:
The material medicine weight proportion of producing 100 kilograms of Monopteri albi anthelmintics is: Fructus Quisqualis 50kg, Rhizoma Osmundae 10kg, Radix Stemonae 10kg, Semen Torreyae 30kg.
Production technology is identical with embodiment 1 with using method.
Embodiment 4:
The primary raw material drug weight proportioning of producing 100 kilograms of Monopteri albi anthelmintics is: Fructus Quisqualis 40kg, Rhizoma Osmundae 15kg, Radix Stemonae 15kg, Semen Torreyae 15kg.Less important material medicine weight proportion is: levamisole 10kg, and adhesive material 5kg, wherein, adhesive material adopts α starch.
Production technology is: primary raw material medicine → drying (105 ℃ of electric heating drying to moisture content are lower than 5%) → pulverizing (hammer crusher) → sieve (60 order steel sieve sieve) → encapsulate by proportioning auxiliary material medicine → mixing (blender the mixings) → subpackage of weighing → add according to the above ratio.
During use it is sneaked in the fish meal and get final product.
Embodiment 5:
The primary raw material drug weight proportioning of producing 100 kilograms of Monopteri albi anthelmintics is: Fructus Quisqualis 30kg, Rhizoma Osmundae 10kg, Radix Stemonae 24kg, Semen Torreyae 20kg.Less important material medicine weight proportion is: levamisole 8kg, and adhesive material 8kg, wherein, adhesive material adopts α starch.
Production technology is identical with embodiment 4 with using method.
Embodiment 6:
The primary raw material drug weight proportioning of producing 100 kilograms of Monopteri albi anthelmintics is: Fructus Quisqualis 50kg, Rhizoma Osmundae 10kg, Radix Stemonae 20kg, Semen Torreyae 10kg.Less important material medicine weight proportion is: levamisole 5kg, and adhesive material 5kg, wherein, adhesive material adopts α starch.
Production technology is identical with embodiment 4 with using method.
Claims (3)
1, a kind of Monopteri albi anthelmintic is characterized in that the medicament that this medicine adopts following weight proportion material medicine to make: Fructus Quisqualis 30-50%, Rhizoma Osmundae 10-30%, Radix Stemonae 10-30%, Semen Torreyae 10-30%.
2,, it is characterized in that described medicament is a powder according to the described Monopteri albi anthelmintic of claim 1.
3, according to the described Monopteri albi anthelmintic of claim 1, it is characterized in that this medicine material medicine weight proportion also comprises the levamisole of 5-10% and the adhesive material of 5-8%, wherein, Fructus Quisqualis, Rhizoma Osmundae, the Radix Stemonae and four kinds of crude drug dryings of Semen Torreyae, pulverizing get final product with levamisole and adhesive material mix homogeneously behind 60 mesh sieves excessively.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410012808 CN1235631C (en) | 2004-03-04 | 2004-03-04 | Anti-parasite for eel |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410012808 CN1235631C (en) | 2004-03-04 | 2004-03-04 | Anti-parasite for eel |
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| Publication Number | Publication Date |
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| CN1559506A true CN1559506A (en) | 2005-01-05 |
| CN1235631C CN1235631C (en) | 2006-01-11 |
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| Application Number | Title | Priority Date | Filing Date |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102886005A (en) * | 2011-07-20 | 2013-01-23 | 天津瑞贝特科技发展有限公司 | Chinese medicinal composition for treating parasites in fishes cultivated in sea fresh water and preparation method thereof |
| CN103656400A (en) * | 2012-09-24 | 2014-03-26 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine composition for treating fish parasitic disease and preparation method thereof |
| CN105327173A (en) * | 2015-11-19 | 2016-02-17 | 集美大学 | Chinese herbal medicine compound additive for preventing and treating fish parasite diseases and production method of Chinese herbal medicine compound additive |
| CN107998318A (en) * | 2018-01-15 | 2018-05-08 | 安徽省农业科学院水产研究所 | A kind of pharmaceutical composition for treating swamp eel enteron aisle parasitic worm |
-
2004
- 2004-03-04 CN CN 200410012808 patent/CN1235631C/en not_active Expired - Fee Related
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102886005A (en) * | 2011-07-20 | 2013-01-23 | 天津瑞贝特科技发展有限公司 | Chinese medicinal composition for treating parasites in fishes cultivated in sea fresh water and preparation method thereof |
| CN103656400A (en) * | 2012-09-24 | 2014-03-26 | 天津瑞贝特科技发展有限公司 | Traditional Chinese medicine composition for treating fish parasitic disease and preparation method thereof |
| CN105327173A (en) * | 2015-11-19 | 2016-02-17 | 集美大学 | Chinese herbal medicine compound additive for preventing and treating fish parasite diseases and production method of Chinese herbal medicine compound additive |
| CN105327173B (en) * | 2015-11-19 | 2019-04-30 | 集美大学 | A kind of Chinese herb compound additive and its production method for preventing and treating eliminating fish parasites disease |
| CN107998318A (en) * | 2018-01-15 | 2018-05-08 | 安徽省农业科学院水产研究所 | A kind of pharmaceutical composition for treating swamp eel enteron aisle parasitic worm |
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| Publication number | Publication date |
|---|---|
| CN1235631C (en) | 2006-01-11 |
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