CN1511544A - Traditional Chinese medicine compound preparation for preventing and treating intestinal adhesion and preparing method - Google Patents
Traditional Chinese medicine compound preparation for preventing and treating intestinal adhesion and preparing method Download PDFInfo
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Abstract
The compound Chinese medicine preparation for preventing and treating intestinal adhesion is oral taken preparation with the extractive of rhubarb, immature bitter orange, magnolia bark, mirabilite and radish seed as the active components and medicinal supplementary material. The medicine has the functions of relieving stasis, eliminating evils, invigorating spleen, drying the wetness evil, activating vital energy and relieving pain, and is used in treating abdominal pain, abdominal distention, vomit, constipation, etc. caused by abdominal operation and adherent ileus and preventing post-operation intestinal adhesion.
Description
Technical field:
The present invention relates to Chinese medicine preparation and preparation method, be specifically related to prevent and treat the compound Chinese medicinal preparation and the preparation method of intestinal adhesion.
Background technology:
Abdominal postoperative, because operation wound, factors such as foreign body stimulation and pathological changes, very easily cause the scorching change of cell, edema, cellulose to ooze out further formation strap, and gastrointestinal function is in disorder, palsy in a period of time, so very easily cause intestinal adhesion to cause the generation of adhesive ileus, stomachache, abdominal distention, vomiting can occur, just disease such as close.
Motherland's medical science is thought the six hollow viscera must keep its unobstructed, then pain.If, cause FU QI being obstructed because of the stagnation of QI, blood stasis, the resistance of the phlegm-damp pathogenic heat stasis of blood, movement of QI being out of order, spleen-QI failing to rise up, failure of stomach-QI to descend, large intestine conduction mistake department, disease such as then can occur pain, bloated, leaf clinically, close.Intestinal obstruction is a kind of commonly encountered diseases of surgery, and adhesive ileus accounts for more than 70% in the intestinal obstruction morbidity.In order to effectively prevent the generation of intestinal adhesion, for many years,, people use ways such as anticoagulant, hormone medicine though adopting such as reducing in the operation in damage to intestinal tube and adjacent organ serous coat, the operation in the soft and art of operation, and the result is all undesirable.
Summary of the invention:
Technical problem to be solved by this invention is utilized theory of Chinese medical science to provide a kind of to have that Tongli is captured, promoting the circulation of QI to relieve pain, the spleen strengthening and damp drying function, the stomachache that treatment abdominal postoperative and simple property adhesive ileus cause, abdominal distention, vomiting, just the new medicine compound preparation of disease such as closes.
The compound Chinese medicinal preparation of prevention disclosed by the invention and treatment intestinal adhesion is by extract that contains Radix Et Rhizoma Rhei, Fructus Aurantii Immaturus, Cortex Magnoliae Officinalis, Natrii Sulfas, Semen Raphani active ingredient and oral formulations that pharmaceutic adjuvant is formed.
At least contain 2～20% (W/W) Radix Et Rhizoma Rhei, 4～25% (W/W) Fructus Aurantii Immaturus, 4～25% (W/W) Cortex Magnoliae Officinalis, 2～20% (W/W) Natrii Sulfas, 4-25% (W/W) Semen Raphani in the described active ingredient prescription; The healthy energy that all the other components are selected from invigorating the spleen and replenishing QI, help damage in the prescription, the medicine of coordinating the actions of various ingredients in a prescription effect is as the Rhizoma Atractylodis Macrocephalae, Poria, Rhizoma Alismatis, Radix Paeoniae Rubra, Radix Glycyrrhizae etc.
It is Radix Et Rhizoma Rhei 2～20%, Fructus Aurantii Immaturus 4～25%, Cortex Magnoliae Officinalis 4～25%, Natrii Sulfas 2～20%, Semen Raphani 4～25%, the Rhizoma Atractylodis Macrocephalae 8～20%, Poria 10～26%, Radix Paeoniae Rubra 10～26%, Rhizoma Alismatis 8～20%, Radix Glycyrrhizae 8～20% that the present invention preferably draws together sexual element percentage by weight prescription.
Oral formulations of the present invention is meant medically acceptable various dosage forms, as granule, tablet, capsule etc.; Described pharmaceutic adjuvant is conventional pharmaceutical adjunct.
Another technical problem to be solved by this invention is the preparation method that discloses above-mentioned compound Chinese medicinal preparation.
The preparation of the compound Chinese medicinal preparation of prevention disclosed by the invention and treatment intestinal adhesion comprises the following steps:
Radix Et Rhizoma Rhei, Cortex Magnoliae Officinalis adopt percolation, use 60% ethanol percolation, collect the first liquid of filtering of 85% times of amount; Continue and filter the liquid decompression recycling ethanol to fluid extract, standby; Fructus Aurantii Immaturus, the Rhizoma Atractylodis Macrocephalae extract volatile oil, and volatile oil comprises with beta-schardinger dextrin-; Medicinal residues and all the other medical materials decoct with water secondary, filter, and filtrate merges with the aqueous solution that extracts after volatile oil distills, be concentrated into fluid extract, add an amount of ethanol precipitate with ethanol, the supernatant decompression recycling ethanol mixes with Radix Et Rhizoma Rhei, Cortex Magnoliae Officinalis fluid extract and Natrii Sulfas to fluid extract, vacuum drying is to doing, be ground into fine powder, mix, add Radix Et Rhizoma Rhei, the Cortex Magnoliae Officinalis liquid of just filtering with beta-schardinger dextrin-inclusion and an amount of dextrin, get active component extract, make oral formulations such as granule, tablet, capsule etc. then according to a conventional method.
A technical problem more to be solved by this invention is to disclose the application of above-mentioned compound Chinese medicinal preparation in preparation prevention and treatment intestinal adhesion medicine.
Medicine of the present invention has that Tongli is captured, the function of the spleen strengthening and damp drying, promoting the circulation of QI to relieve pain, is mainly used in stomachache that treatment abdominal postoperative and simple property adhesive ileus cause, abdominal distention, vomiting, just disease such as closes; And the effect of prevention abdominal post-operation intestinal adhesion arranged.
Theoretical system by the traditional Chinese medical science can be divided into three types to adhesive ileus: painful abdominal mass junction type, stasis of blood junction type, cellulitis junction type.Preparation of the present invention can be used for treating the painful abdominal mass junction type and the stomachache, the abdominal distention that occur, just closes, vows flow of QI being obstructed, nausea and vomiting, diseases such as red tongue, Tai Bai or thick greasy, deep and stringy pulse.
Using dosage is equivalent to crude drug amount 31g for take at every turn, every day secondary.
Abdominal postoperative, because operation wound, foreign body stimulates and factor such as pathological changes, gastrointestinal function is in disorder, palsy in a period of time, stomachache, abdominal distention, nausea and vomiting often occur, just card such as close; This kind state is as can not in time correcting, and connective tissue discharges vaso-active substance under the serous coat, makes telangiectasis, permeability increases, contain a large amount of fibrinogenic body fluid and join, promptly and the loose adhesion of solidificating fiber disposition, the serosal surface of adjacent internal organs is sticked together.Just have fibroblast and angiogenesis if the adhesion of fiber disposition is absorbed, and then form firm fibrous adhesion.Because intestinal adhesion causes enteric cavity narrow, pathway freely can not form adhesive ileus again.Motherland's medical science thinks that burnt category during abdominal part belongs to includes internal organs such as taste, large intestine small intestinal, gallbladder, bladder." so six internal organs person, excreting foodstuff instead of storing essence is FU-organ being filled with foodstuff but inability to be full of essence-QI ".The six hollow viscera must keep its unobstructed, stagnation of QI and blood may bring about pain.If the intestines and stomach damp-heat accumulation, blood stasis or congenital anomaly is felt eating and drinking without temperance again, overworking causes obstruction of flow of QI due to the golden cutter, stagnation of QI internal organs excess syndrome then can appear in abnormal ascending-descending of QI.Tend to occur stomachache, abdominal distention feeling of fullness clinically, just close, vow flow of QI being obstructed, nausea and vomiting, red tongue, tongue fur is white or thickly be bored with diseases such as deep and stringy pulse.This external mechanical factor of performing the operation also is a kind of damage to the human righteousness, so the stagnation of QI excess syndrome that is caused after the iatrotechnics is when Tongli is captured, promoting the circulation of QI to relieve pain, the spleen strengthening and damp drying.
Radix Et Rhizoma Rhei in the preparation prescription of the present invention, bitter cold is gone into spleen, stomach, large intestine channel.The heat and toxic materials clearing away intestinal stasis relieving, " cleanse the intestines and stomach, push away Chen Zhixin " (" herbal classic "), " it is descending to land vertically, walk and do not keep " (" medicine justice ") with by excess pathogen, makes internal organs be so incensed that logical, so be monarch drug.Fructus Aurantii Immaturus, hardship is slightly cold, and goes into the taste warp.Dispelling the stagnated QI looses and amasss the painful abdominal mass that disappears, " removing the cold and heat knot " (" herbal classic "); Cortex Magnoliae Officinalis, bitter, hot, warm, go into spleen, stomach, large intestine channel, the circulation of qi promoting intestinal stasis relieving.The bitter in the mouth therapeutic method to keep the adverse QI flowing downwards, warm in nature and capable diffusing.Can remove the gas that stagnates in the stomach, again can dry spleen wet, full the removing of loosing be expanded.Natrii Sulfas, hot, salty, bitter, tremble with fear, go into stomach, large intestine, tri-jiao channel.Moistening for dryness and softening hard mass also " is washed place dirt in the intestinal " (" pearl sac ").Semen Raphani, hot, sweet, put down, go into spleen, stomach warp, be longer than regulating QI to remove the stagnation, help digestion to remove and expand.Last four flavors be ministerial drug altogether, and principal drug assistance logical is captured to increase, circulation of qi promoting intestinal stasis relieving, the painful abdominal mass that disappears be except that bloated effect.First three flavor medicine and monarch drug Radix Et Rhizoma Rhei are combined into DACHENGQI TANG in the ministerial drug, are the name side that Tongli is captured.Operation must injure healthy energy, and purging FU-organs rushes down the purgation product must undermine QI of the spleen and stomach, so use the Rhizoma Atractylodis Macrocephalae in the side, gets its bitter in the mouth, and sweet using warming therapy, invigorating the spleen and benefiting QI, and can " dehumidify ... expectorant " (" pearl sac "); Poria, sweet flat, the spleen invigorating invigorating middle warmer, " every middle expectorant water " (" Mingyi Bielu ") also disappears; Rhizoma Alismatis, sweet cold, promoting diuresis to eliminate damp pathogen has the effect of " supporting the five internal organs, physical strength profiting " " herbal classic " again; Radix Paeoniae Rubra, " therapeutic method to keep the adverse QI flowing downwards ... clear and coherent blood vessels " (" Bencao Pinhui Jingyao "), promoting the circulation of blood gas and slow pain.Last four medicines are adjuvant drug, can assist a ruler in governing a country the monarch and his subjects' the spleen QI invigorating of building up one's health by taking tonic, and help damage healthy energy, and the high violent property of cushion can assist the monarch and his subjects' medicine to dispel phlegm-damp stasis of blood heresy again.Radix Glycyrrhizae, sweet flat, coordinating the actions of various ingredients in a prescription is messenger drug, " slow its heat that the medicine of a warm nature gets, cold slow its that gets this trembled with fear, the assorted mutually person of cold and heat, the De Qiping that uses ".
This side's compatibility is a reinforcement and elimination in combination, treating both the principal and secondary aspects of a disease, and it is proper to fill a prescription, and plays Tongli altogether and captures, promoting the circulation of QI to relieve pain, the function of the spleen strengthening and damp drying.Thereby can reach no adhesion and block and can prevent, there is adhesion to block the purpose that can control.
Carry out relevant toxicity, drug effect and clinical trial with preparation of the present invention:
One, acute toxicity test
With 496gkg
-1The preparation of the present invention of (amounting to into the crude drug amount) dosage (the more logical granule extractum of art) row mice lavage administration, performances such as though fewly movingly, tired crouching appear in visible animal, does not moisten loose stool, hair, it is dead not have 1 example, and its conclusion is, this dosage is equivalent to 480 times of Coming-of-Age Day clothes dosage, and true border is nontoxic.
Two, long term toxicity test
With the rat is animal subject, divides the distilled water matched group, the more logical low dose group (6.25gKg of art
-1), dosage group (12.5gKg during art is more logical
-1), the more logical high dose group (25Kg of art
-1).Gastric infusion is 96 days continuously, the result removes and can cause rat diarrhoea, the high dose group the weight of animals increasess slowly outside (with the matched group there was no significant difference), and hematology such as rat erythrocyte sum (RBC), leukocyte total (WBC), hemoglobin content (HGB), leukocyte differential count, GPT, AST, ALP, Cre, BUN, TP, ALB, GLU, T-CHO and biochemical indexes are not all had obvious influence.To main organs such as liver, the heart, spleen, lung, kidney, brain, adrenal gland, testis, uterus and ovaries, there is no obvious change from outward appearance, internal organs organ weight/power ratio and histopathological examination.The result proves that the more logical granule of art is taken to animal for a long time and does not see that overt toxicity, medication are safe.
Three, the test of pesticide effectiveness
1, experiment material
1.1 be subjected to the more logical granule of reagent thing preparation art of the present invention, every 10g granule contains crude drug amount 31g.Usage and consumption: oral, a 10g, 2 times on the one.In pharmacodynamic experiment, be made into the suspension use of desired concn with distilled water.
1.2 positive control drug gastrointestine recovery cream (post-equalization cream), usage and consumption: oral, a 15～30g, 2 times on the one.Be mixed with the suspension use of desired concn in the experiment with distilled water.Neostigmine methylsulfate injection (neostigmine), anisodamine, aspirin sheet, hydrocortisone injection (hydrogen can).
1.3 reagent noradrenaline bitartrate injection (NA), dimethylbenzene (analytical pure), india ink, gentamicin injection liquid.
1.4 laboratory animal Kunming kind white mice, body weight 18-24g.The Wistar rat, body weight 150-250g.
2, experimental result:
2.1.1 influence to the small intestine movement of mice ahead running
The more logical granule of table 1 art is to the influence (n=12) of small intestine movement of mice ahead running
Drug dose (gKg-1) carbon powder propelling rate (%, X ± S)
Distilled water-48.1 ± 11.3
Neostigmine 1.2 * 10
-476.0 ± 10.0
*
Post-equalization cream 3.02 66.7 ± 11.3
*
Art more leads to 1.55 59.2 ± 9.9
*
3.10 65.5±11.8
**
6.20 68.3±13.3
**
Compare with the distilled water group:
*P＜0.01
The result shows that each administration group and distilled water group compare, and carbon powder propelling rate all has the difference (P＜0.01) of highly significant, illustrates that the more logical granule of art has the effect that promotes mice intestinal ahead running.
2.1.2 influence to operation wound mouse small intestine ahead running
The more logical granule of table 2 art is to the propulsive influence of sham operated intestinal tube mechanical damage mice (n=12)
Drug dose (gKg-1) carbon powder propelling rate (%, X ± S)
Distilled water-41.4 ± 12.6
Neostigmine 1.2 * 10
-474.1 ± 10.1
*
Post-equalization cream 3.02 65.9 ± 11.7
*
Art more leads to 1.55 55.4 ± 12.5
*
3.10 66.2±12.5
**
6.20 66.5±12.0
**
Compare with the distilled water group:
*P＜0.01
By table 2 as seen, the more logical granule of art has obvious facilitation for sham operated intestinal tube mechanical damage mice intestinal ahead running, with distilled water group comparison carbon powder propelling rate the difference (P＜0.01) of highly significant is arranged.
2.2 influence to experimental intestinal adhesion model
2.2.1 influence to rat experiment intestinal adhesion model
Table 3 abdominal adhesions evaluation criteria
Grade abdominal adhesions situation
0 does not have adhesion fully;
I has only adhesion between intestinal tube and mesentery, nethike embrane.Adhesion place is less, and adhesion is loose and separately easy;
II has adhesion between intestinal tube and intestinal tube.Adhesion place is more, and adhesion is fine and close and separately difficult;
III has adhesion between intestinal tube and intestinal tube.Adhesion place is a lot, the adhesion densification, even adhesion is agglomerating.
The more logical granule of table 4 art is to the influence (n=15) of rat experiment intestinal adhesion
Drug dose (gkg-1) administration time (d) adhesion grade
0 I II III Ri and 99% fiducial limit
Distilled water-1-7 013 11 0.500 (0.3300-0.670)
Post-equalization cream 3.02 1-7 3642 0.858 (0.688-1.208)
*
More logical 1.55 1-7 2454 0.767 (0.597-0.937) of art
*
3.10 1-7 3 7 3 2 0.867(0.697-1.037)
**
6.20 1-7 2 6 4 3 0.816(0.646-0.986)
*
Compare with the distilled water group:
*P＜0.05;
*P＜0.01
Table 4 is preceding 5 groups of experimental datas.Experimental result shows that more logical three the dosage groups of granule of art are all light than distilled water group with the intestinal adhesion degree of gastrointestine recovery cream group, and range estimation adhesion scope is little, and compactness extent is loose, and minority animal intestinal tube heals, and serous coat is smooth, does not stick together fully.And distilled water group major part has intestinal tube and adhesion of abdominal wall, and adhesion fine and close be difficult for dialling from, indivedual even adhesion is agglomerating.Analyze through Ridit, compare, more logical low dosage of art and high dose group significant difference (P＜0.05), middle dosage group and post-equalization cream group difference highly significant (P＜0.01) with the distilled water group.
The more logical granule of table 5 art is to the influence (n=15) of rat experiment intestinal adhesion
Drug dose (gkg-1) administration time (d) adhesion grade Ri and 99% fiducial limit
0 I II III
Distilled water-7-13 013 11 0.500 (0.381-0.619)
Post-equalization cream 3.02 7-13 1464 0.756 (0.636-0.875)
*
More logical 1.55 7-13 0375 0.704 (0.585-0.824) of art
3.10 7-13 2 2 8 3 0.780(0.661-0.899)
*
6.20 7-1?3 0 2 9 4 0.727(0.608-0.846)
Compare with the distilled water group:
*P＜0.05
By table 5 as seen, more logical granule of art and gastrointestine recovery cream adopt postoperative beginning in the 7th day administration, for rat experiment intestinal adhesion certain therapeutical effect are arranged.Compare with matched group, there were significant differences (P＜0.05) for the average Ridit value of dosage group during post-equalization cream group and art were more logical.Though the more logical low dosage of art and high dose group and matched group comparison not statistically significant (P＞0.05), the average Ridit value of its adhesion grade also has rising trend.The result is not difficult to find out with reference to table 4, and postoperative beginning in 24 hours administration curative effect is better than beginning administration in the 7th day.
2.2.2 influence to rabbit experiment intestinal adhesion model
The more logical granule of table 6 art is to the influence of rabbit experiment intestinal adhesion
The routine number administration time of drug dose (gkg-1) (d) adhesion grade
0 I II III Ri and 99% fiducial limit
Distilled water-11 1-7 0029 0.500 (0.317-0.683)
Post-equalization cream 3.02 12 1-7 1542 0.871 (0.696-1.046)
*
More logical 1.55 12 1-7 1533 0.833 (0.718-0.948) of art
*
3.10 12 1-7 3 4 3 2 0.879(0.704-1.054)
**
6.20 11 1-7 1 4 3 3 0.814(0.692-0.936)
*
Compare with the distilled water group:
*P＜0.05;
*P＜0.01
The more logical granule of table 7 art is to the influence of rabbit experiment intestinal adhesion
The routine number administration time of drug dose (gkg-1) (d) adhesion grade
0 I II III Ri and 99% fiducial limit
Distilled water-12 7-13 0039 0.500 (0.388-0.612)
Post-equalization cream 3.02 11 7-13 0434 0.765 (0.648-0.885)
*
More logical 1.55 11 7-13 0524 0.773 (0.654-0.892) of art
*
3.10 12 7-13 0 5 3 4 0.785(0.672-0.897)
*
6.20 12 7-13 0 2 6 4 0.764(0.652-0.876)
*
Compare with the distilled water group:
*P＜0.05;
*P＜0.01
Experimental result, the most of animal intestine adhesion of matched group area is big, and intestinal tube and peritoneum and adhesion of abdominal wall are arranged, the adhesion densification, be difficult to dial from, the adhesion of part animal is agglomerating.The intestinal adhesion situation of each administration group is obviously improved, and shows the be formed with inhibitory action of the more logical granule of art for rabbit experiment intestinal adhesion.It is obvious that the abdominal adhesions condition improved is respectively organized in postoperative 1-7 days administration, and minority animal wound healing is good, does not stick together.More all there were significant differences (P＜0.05) for average Ridit value and the matched group of each group of postoperative administration in 7～13 days, but the adhesion situation respectively organizes not as postoperative 1-7 days administration and improve significantly, points out for the treatment administration as early as possible of abdominal post-operation intestinal adhesion to well.
2.3 influence to the rat mesentery vascular flow
The more logical granule of table 8 art is to the influence (n=6) of rat mesentery blood capillary blood flow
Medication medication concentration (mgmL
-1) the blood capillary restoration of blood flow time (min, X ± S)
Normal saline (NS)-13.58 ± 1.47
Anisodamine 5 2.65 ± 0.62
*
Post-equalization cream 75.5 7.34 ± 1.57
*
Art more leads to 38.75 7.24 ± 1.99
*
77.50 6.79±1.94
**
155.00 5.97±1.55
**
Compare with the NS group:
*P＜0.01
The result instils after the NA, and generally the mesenteric microvessels blood flow is stagnated in the time of 20 seconds to 1 minute.The instillation NS group blood capillary restoration of blood flow time is 13.58 ± 1.47min.Each administration group all obviously shortens the blood capillary restoration of blood flow time, organizes the difference (P＜0.01) that highly significant is more all arranged with NS.Show that the more logical granule of art improves significantly for the rat mesentery microcirculation disturbance that NA causes.
2.4 antiinflammatory action
2.4.1 xylol brings out the influence of mice auricle swelling
The more logical granule xylol of table 9 art brings out the influence (n=12) of mice auricle swelling
Drug dose (gKg
-1) auricle swelling degree (mg, X ± S)
Distilled water-14.34 ± 3.60
Aspirin 0.2 4.85 ± 1.70
*
Post-equalization cream 6.04 5.68 ± 1.66
*
Art more leads to 3.10 7.65 ± 2.85
*
6.20 5.48±2.20
**
12.40 5.06±2.82
**
Compare with the distilled water group:
*P＜0.01
Table 9 data show that the more logical granule xylol of art brings out mice auricle swelling obvious suppression effect (P＜0.01).
2.4.2 to the bullate influence of rat granuloma
The more logical granule of table 10 art is to the bullate influence of rat granuloma (n=8)
Drug dose (gKg
-1) granulation tissue weight/power ratio (mgkg
-1, X ± S)
Distilled water-557 ± 97
Hydrogen can 0.02 294 ± 69
*
Post-equalization cream 6.04 447 ± 86
*
Art more leads to 3.10 432 ± 92
*
6.20 438±90
*
12.40 425±105
*
Compare with the distilled water group:
*P＜0.05;
*P＜0.01
The result shows, more logical granule of art and gastrointestine recovery cream all obviously suppress the rat granulation tissue hyperplasia, and compares that there were significant differences (P＜0.05), but its effect is than a little less than the hydrocortisone.
2.4.3 influence to capillary permeability
The more logical granule of table 11 art is to the influence (n=10) of mice capillary permeability
Drug dose (gKg
-1) peritoneal lavage fluid OD value (X ± S)
Distilled water-0.166 ± 0.034
Post-equalization cream 3.02 0.086 ± 0.014
*
Art more leads to 1.55 0.072 ± 0.021
*
3.10 0.073±0.016
**
6.20 0.078±0.021
**
Compare with the distilled water group:
*P＜0.01
By table 11 data as seen, each administration group peritoneal lavage fluid OD value and matched group more all have the difference (P＜0.01) of highly significant, show that more logical granule of art and gastrointestine recovery cream can obviously reduce the mouse peritoneal capillary permeability, reduce inflammatory exudation.
2.5 bacteriostasis
The more logical granule bacteriostasis experimental result (inhibition zone diameter: mm) of table 12 art
Bacterium streptococcus escherichia coli are read bacterium in vain in medicine gold Portugal
Physiologic dose brine 7877
Ampicillin 36 33 26-
Art more leads to (30.2%) 17 12 17 9
Art more leads to (15.5%) 9898
Art more leads to (31.0%) 98 11 7
Art more leads to (62.0%) 19 14 18 10
Evaluation criterion: bacteriostatic diameter≤10mm is a drug resistance; 11-16mm is a medium sensitivity;＞16mm is extremely sensitive.
The more logical granule (62%) of art all has inhibitory action with gastrointestine recovery cream for staphylococcus aureus, micrococcus scarlatinae and escherichia coli as seen from Table 12, and is relatively poor to the oidiomycetic bacteriostasis of white.
Conclusion:
The more logical granule of art is with 1.55,3.10,6.20 gastric infusions, for the intestinal ahead running of normal mouse and sham operated intestinal tube mechanical damage mice obvious facilitation arranged, with matched group relatively carbon powder advance obviously raise (P＜0.01).For rat experiment intestinal adhesion model, postoperative 1-7 days gastric infusion, inhibitory action is (P＜0.05 or P＜0.01) significantly; Postoperative 7-13 days administration, 3.10gKg
-1Dosage group inhibitory action is (P＜0.05) obviously, though two other dosage group not statistically signigicant, its intestinal adhesion degree is reduction trend.With 1.55,3.10,6.20gKg
-1Irritate stomach to rabbit, for experimental intestinal adhesion model, postoperative 1-7 days administration is for the obvious inhibitory action of being formed with of intestinal adhesion (P＜0.05; P＜0.01).More also there were significant differences with matched group (P＜0.05) for each group of postoperative 7-13 days administration, but the improvement degree of abdominal adhesions situation each is organized not as postoperative 1-7 days administration, point out this product should be with administration as early as possible for well for the treatment of abdominal post-operation adhesion.With the administration of part instillation mode, the more logical granule of art is stagnated for rat mesentery blood capillary blood flow due to the norepinephrine, has the effect (P＜0.01) of tangible shortening restoration of blood flow time, shows that the more logical granule of art has the effect of microcirculation improvement.With 3.10,6.20,12.4gKg
-1The more logical granule of gastric infusion art is for caused by dimethylbenzene xylene white mice auricle edema, the rat granulation tissue hyperplasia is brought out in implantation for cotton balls significant inhibitory effect, strengthen the inhibitory action that highly significant is also arranged for acetic acid induced mice abdominal cavity capillary permeability, thereby reduce inflammatory exudation.External bacteriostatic experiment shows that more logical granule 62% concentration of art has certain inhibitory action for staphylococcus aureus, micrococcus scarlatinae and escherichia coli, and is not obvious for the Candida albicans inhibitory action.
Four, clinical observation
By preventative observed result prompting to various different patient with operation 63 examples, observe 1 group of (liver and gall operation) total effective rate and obvious effective rate and be respectively 93.94%, 30.30%, borborygmus, aerofluxus and defecation time are respectively 39.8 ± 3.2,45.0 ± 2.8,81.3 ± 4.6 hours, curative effect was better than matched group.Observe 2 groups of (the scorching patient with operation of acute blue tail) total effective rates and obvious effective rate and be respectively 96.67%, 50%, borborygmus, aerofluxus and defecation time are respectively 14.5 ± 1.5, and 24.3 ± 2.6,44.5 ± 3.2, curative effect also is better than matched group.
Above-mentioned result of the test shows that preparation of the present invention has formation, microcirculation improvement, antiinflammatory and the bacteriostasis of remarkable promotion intestinal smooth muscle motion, inhibition abdominal post-operation intestinal adhesion.
The specific embodiment
The preparation of embodiment 1 active component extract
Prescription: Radix Et Rhizoma Rhei 150g, Fructus Aurantii Immaturus 300g, Cortex Magnoliae Officinalis 300g, Natrii Sulfas 150g, Semen Raphani 300g, Rhizoma Atractylodis Macrocephalae 400g, Poria 500g, Radix Paeoniae Rubra 400g, Rhizoma Alismatis 300g, Radix Glycyrrhizae 300g,
Radix Et Rhizoma Rhei, Cortex Magnoliae Officinalis adopt percolation, use 60% ethanol percolation, collect the first liquid of filtering of 85% times of amount; Continue and filter the liquid decompression recycling ethanol to fluid extract, standby; Fructus Aurantii Immaturus, the Rhizoma Atractylodis Macrocephalae extract volatile oil, and volatile oil comprises with beta-schardinger dextrin-; Medicinal residues and all the other medical materials decoct with water secondary, filter, filtrate merges with the aqueous solution that extracts after volatile oil distills, and is concentrated into fluid extract, add an amount of ethanol precipitate with ethanol, the supernatant decompression recycling ethanol mixes with Radix Et Rhizoma Rhei, Cortex Magnoliae Officinalis fluid extract and Natrii Sulfas to fluid extract, and vacuum drying is to doing, be ground into fine powder, mix with beta-schardinger dextrin-inclusion and an amount of dextrin, add Radix Et Rhizoma Rhei, the Cortex Magnoliae Officinalis liquid of just filtering, active component extract.
Embodiment 2
Prescription: Radix Et Rhizoma Rhei 140g, Fructus Aurantii Immaturus 280g, Cortex Magnoliae Officinalis 280g, Natrii Sulfas 140g, Semen Raphani 350g, Rhizoma Atractylodis Macrocephalae 400g, Poria 460g, Radix Paeoniae Rubra 350g, Radix Glycyrrhizae 350g, Rhizoma Alismatis 350g
Extraction of active ingredients thing preparation method is with embodiment 1.Extraction of active ingredients thing that makes and an amount of dextrin, ethanol mixing granulation, drying makes into 1000g, and packing promptly gets granule.
Embodiment 3
Prescription: Radix Et Rhizoma Rhei 45g, Fructus Aurantii Immaturus 90g, Cortex Magnoliae Officinalis 90g, Natrii Sulfas 45g, Semen Raphani 90g, Rhizoma Atractylodis Macrocephalae 120g, Poria 150g, Radix Paeoniae Rubra 120g, Radix Glycyrrhizae 90g, Rhizoma Alismatis 90g
Extraction of active ingredients thing preparation method is with embodiment 1.Extraction of active ingredients thing that makes and an amount of dextrin, ethanol mixing granulation, drying makes into 300g, divides encapsulatedly, makes 1000 capsules.
Embodiment 4
Prescription: Radix Et Rhizoma Rhei 37.5g, Fructus Aurantii Immaturus 75g, Cortex Magnoliae Officinalis 75g, Natrii Sulfas 37.5g, Semen Raphani 75g, Rhizoma Atractylodis Macrocephalae 100g, Poria 125g, Radix Paeoniae Rubra 100g, Radix Glycyrrhizae 75g, Rhizoma Alismatis 75g
Extraction of active ingredients thing preparation method is with embodiment 1.Extraction of active ingredients thing that makes and an amount of dextrin, starch, ethanol mixing granulation, drying, granulate, tabletting, coating promptly get 1000.
Claims (8)
1, the compound Chinese medicinal preparation that has prevention and treatment intestinal adhesion effect is characterized in that said preparation is by extract that contains Radix Et Rhizoma Rhei, Fructus Aurantii Immaturus, Cortex Magnoliae Officinalis, Natrii Sulfas, Semen Raphani active ingredient and oral formulations that pharmaceutic adjuvant is formed.
2, compound Chinese medicinal preparation according to claim 1 is characterized in that containing at least in the wherein said active ingredient prescription 2～20%W/W Radix Et Rhizoma Rhei, 4～25%W/W Fructus Aurantii Immaturus, 4～25%W/W Cortex Magnoliae Officinalis, 2～20%W/W Natrii Sulfas, 4-25%W/W Semen Raphani.
3, compound Chinese medicinal preparation according to claim 1 and 2 is characterized in that the healthy energy that all the other components are selected from invigorating the spleen and replenishing QI, help damage in the wherein said active ingredient prescription, the medicine of coordinating the actions of various ingredients in a prescription effect.
4, compound Chinese medicinal preparation according to claim 1 is characterized in that the active ingredient percentage by weight is Radix Et Rhizoma Rhei 2～20%, Fructus Aurantii Immaturus 4～25%, Cortex Magnoliae Officinalis 4～25%, Natrii Sulfas 2～20%, Semen Raphani 4～25%, the Rhizoma Atractylodis Macrocephalae 8～20%, Poria 10～26%, Radix Paeoniae Rubra 10～26%, Rhizoma Alismatis 8～20%, Radix Glycyrrhizae 8～20% in the wherein said active ingredient prescription.
5, compound Chinese medicinal preparation according to claim 1 is characterized in that wherein said oral formulations is meant medically acceptable various dosage forms, as granule, tablet, capsule; Described pharmaceutic adjuvant is conventional pharmaceutical adjunct.
6, the preparation method of compound Chinese medicinal preparation according to claim 1 is characterized in that the preparation of wherein said compound preparation comprises the following steps:
Radix Et Rhizoma Rhei, Cortex Magnoliae Officinalis adopt percolation, use 60% ethanol percolation, collect the first liquid of filtering of 85% times of amount; Continue and filter the liquid decompression recycling ethanol to fluid extract, standby; Fructus Aurantii Immaturus, the Rhizoma Atractylodis Macrocephalae extract volatile oil, and volatile oil comprises with beta-schardinger dextrin-; Medicinal residues and all the other medical materials decoct with water secondary, filter, and filtrate merges with the aqueous solution that extracts after volatile oil distills, be concentrated into fluid extract, add an amount of ethanol precipitate with ethanol, the supernatant decompression recycling ethanol mixes with Radix Et Rhizoma Rhei, Cortex Magnoliae Officinalis fluid extract and Natrii Sulfas to fluid extract, vacuum drying is to doing, be ground into fine powder, mix, add Radix Et Rhizoma Rhei, the Cortex Magnoliae Officinalis liquid of just filtering with beta-schardinger dextrin-inclusion and an amount of dextrin, get active component extract, make oral formulations then according to a conventional method.
7, the application of compound Chinese medicinal preparation according to claim 1 in preparation prevention and treatment intestinal adhesion medicine.
8, the application of compound Chinese medicinal preparation according to claim 7 is characterized in that stomachache that wherein said drug main will be used for the treatment of abdominal postoperative and simple property adhesive ileus and cause, abdominal distention, vomiting, just disease such as closes; And the effect of prevention abdominal post-operation intestinal adhesion arranged.
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| CN 02151725 CN1278718C (en) | 2002-12-27 | 2002-12-27 | Traditional Chinese medicine compound preparation for preventing and treating intestinal adhesion and preparing method |
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Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100563680C (en) * | 2006-12-19 | 2009-12-02 | 南京中医药大学 | Chinese medicine preparation and the method for making and the application thereof of prevention and treatment intestinal adhesion after abdominal cavity operation |
| CN101229280B (en) * | 2008-02-01 | 2010-12-08 | 韩峰祥 | Chinese traditional medicine preparation for treating intestinal obstruction |
| CN102614449A (en) * | 2012-04-06 | 2012-08-01 | 王爱玉 | Chinese herbal preparation for curing intestinal adhesion |
| CN102688325A (en) * | 2012-06-25 | 2012-09-26 | 西安新通药物研究有限公司 | Chinese medicinal composition for gastroenteropathy and preparation method thereof |
| CN103239533A (en) * | 2013-05-10 | 2013-08-14 | 山东省中医药研究院 | Flavoured qi infusing decoction for treating simple intestinal obstruction and preparation method thereof |
| CN103536866A (en) * | 2013-11-12 | 2014-01-29 | 崔合芳 | Traditional Chinese medicine composition for treating inflammatory intestinal obstruction after abdominal operation and application thereof |
| CN103933177A (en) * | 2014-05-09 | 2014-07-23 | 张艳梅 | Preparation method of medicament for treating intestinal obstruction |
| CN104257751A (en) * | 2014-09-16 | 2015-01-07 | 苏州市天灵中药饮片有限公司 | Traditional Chinese medicine extract for treating gastritis and preparation method thereof |
| CN113209205A (en) * | 2021-06-18 | 2021-08-06 | 上海市第七人民医院(上海中医药大学附属第七人民医院) | Traditional Chinese medicine composition for treating critically ill gastrointestinal dysfunction and preparation method thereof |
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| CN103191233A (en) * | 2012-01-08 | 2013-07-10 | 张海军 | Adhesion-dredging soup for treating adhesive intestinal obstruction and preparation method thereof |
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- 2002-12-27 CN CN 02151725 patent/CN1278718C/en not_active Expired - Lifetime
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100563680C (en) * | 2006-12-19 | 2009-12-02 | 南京中医药大学 | Chinese medicine preparation and the method for making and the application thereof of prevention and treatment intestinal adhesion after abdominal cavity operation |
| CN101229280B (en) * | 2008-02-01 | 2010-12-08 | 韩峰祥 | Chinese traditional medicine preparation for treating intestinal obstruction |
| CN102614449A (en) * | 2012-04-06 | 2012-08-01 | 王爱玉 | Chinese herbal preparation for curing intestinal adhesion |
| CN102688325A (en) * | 2012-06-25 | 2012-09-26 | 西安新通药物研究有限公司 | Chinese medicinal composition for gastroenteropathy and preparation method thereof |
| CN103239533A (en) * | 2013-05-10 | 2013-08-14 | 山东省中医药研究院 | Flavoured qi infusing decoction for treating simple intestinal obstruction and preparation method thereof |
| CN103536866A (en) * | 2013-11-12 | 2014-01-29 | 崔合芳 | Traditional Chinese medicine composition for treating inflammatory intestinal obstruction after abdominal operation and application thereof |
| CN103536866B (en) * | 2013-11-12 | 2015-01-21 | 崔合芳 | Traditional Chinese medicine composition for treating inflammatory intestinal obstruction after abdominal operation and application thereof |
| CN103933177A (en) * | 2014-05-09 | 2014-07-23 | 张艳梅 | Preparation method of medicament for treating intestinal obstruction |
| CN104257751A (en) * | 2014-09-16 | 2015-01-07 | 苏州市天灵中药饮片有限公司 | Traditional Chinese medicine extract for treating gastritis and preparation method thereof |
| CN113209205A (en) * | 2021-06-18 | 2021-08-06 | 上海市第七人民医院(上海中医药大学附属第七人民医院) | Traditional Chinese medicine composition for treating critically ill gastrointestinal dysfunction and preparation method thereof |
| CN114259495A (en) * | 2022-01-24 | 2022-04-01 | 范德里希(上海)生物科技有限公司 | Application of neostigmine injection in treating hemorrhagic shock diseases |
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| CN1278718C (en) | 2006-10-11 |
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