CN1506094A - Elsholizia extract granule for treating diarrhea and its prepn - Google Patents
Elsholizia extract granule for treating diarrhea and its prepn Download PDFInfo
- Publication number
- CN1506094A CN1506094A CNA021539960A CN02153996A CN1506094A CN 1506094 A CN1506094 A CN 1506094A CN A021539960 A CNA021539960 A CN A021539960A CN 02153996 A CN02153996 A CN 02153996A CN 1506094 A CN1506094 A CN 1506094A
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- China
- Prior art keywords
- add
- preparation
- thymol
- methanol
- reference substance
- Prior art date
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- Granted
Links
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- 206010012735 Diarrhoea Diseases 0.000 title abstract description 16
- 239000000284 extract Substances 0.000 title description 6
- 238000002360 preparation method Methods 0.000 claims abstract description 28
- 239000000341 volatile oil Substances 0.000 claims abstract description 12
- 238000003908 quality control method Methods 0.000 claims abstract description 7
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Landscapes
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention discloses one kind of elsholizia volatile oil granule preparation and its quality control method. The elsholizia volatile oil granule preparation of the present invention have the functions of resisting virus, resisting bacteria, resisting inflammation, stopping diarrhea, relieving pain and allaying fever, and can also suppress sthenic gastrointestinal function.
Description
Technical field
The present invention relates to a kind of Chinese medicine extract preparation and preparation method thereof, particularly relate to a kind of treatment diarrheal Herba Moslae extract granular preparation and preparation method thereof.
Background technology
Diarrhoea (infectious, non-infectious) sickness rate height, also higher in the certain areas case fatality rate, be transworld serious problems.The traditional Chinese medical science is to the then determination of treatment based on pathogenesis obtained through differentiation of symptoms and signs of carrying out of diarrheal treatment more, cold-damp syndrome person huoxiang zhengqi powder master it, damp-heat syndrome person GEGEN QINLIAN TANG master it, dyspepsia card person lenitive pill master it, stagnation of liver-QI takes advantage of spleen person's tongxieyao formula master it, weakness of the spleen and stomach person SHENLING BAISHU SAN master it, kidney yang deficiency card person SISHEN WAN master it.So also there is the soil single-prescription of employing that gastroenteritis is carried out the treatment according to differentiation of diseases person.In the last few years, the report of Chinese traditional treatment viral diarrhea was many, but it is less to form the new drug person.The characteristics of Chinese medicine are to be good at recuperating gastrointestinal tract function, few side effects, but the action intensity of pathogen slightly is inferior to Western medicine.How modern medicine treats with the physical exercise therapy of antimicrobial drug fluid,matching infectious diarrhea, has obviously improved curative effect.The research of nearly microbial ecological agent has during the last ten years caused scholar's attention with application, has carried out vaccine research simultaneously, but owing to cause that the diarrheal pathogen is a lot, lacks cross immunity each other, so the development of vaccine is made slow progress always.Along with going deep into of research, also more and more clearer to the toxic and side effects of antimicrobial drug, as toxicity and gastrointestinal reaction, anaphylaxis and superinfection etc. to kidney, liver, neural spirit, blood.At present viral diarrhea is still lacked specific medicine, the report that external useful lactobacillus, interferon, virazole etc. are treated, domestic useful viral infection chicken, the preparation antibody preparation carries out therapist from ovum gallinaceum then, but still among research and development.In recent years developed the rotavirus live vaccine, among applying.According to the data of WHO, it is inferior that the whole world annual diarrhoea case can reach 30-50 hundred million examples, has 500-1000 ten thousand cases dead because of serious diarrhoea approximately.Inferior, non-, draw the area below 5 years old among the child, annual diarrhoea person is taken place more than 1,000,000,000 person-times, the dead about 5,000,000.According to reports, China suffers from diarrhoea for annual about 8.36 hundred million person-times, and wherein child below 5 years old accounts for 2.09 hundred million person-times, accounts for 1/4.According to Ministry of Public Health in 1993 report, China's acute gastroenteritis two all prevalences are that 11.74%, two all acute diseases constitute by the disease kind and account for 8.36%, come after acute nasopharyngitis and the influenza, occupy ill the 3rd of two weeks of the resident of urban and urual areas of whole country.Rotavirus is to be found by Bishop etc. in 1973.1.4 hundred million person-times of rotavirus diarrhea can take place every year in whole world child below 5 years old, and death can reach 1,000,000 people.Rotavirus is the important cause of disease of infantile diarrhea, has found to cause the one-tenth Human reoviruslike agent of adult diarrhea afterwards again.
Summary of the invention
The object of the invention is to provide a kind of treatment diarrheal Herba Moslae extract preparation.
The object of the invention also is to provide the method for quality control of Herba Moslae and extract thereof.
The present invention seeks to be achieved through the following technical solutions:
The preparation of Herba Moslae volatile oil: the Herba Moslae herb, cutting adds 6-10 times of water gaging, and vapor distillation extracted 4-8 hour, isolated the volatilization oil reservoir, promptly.
Volatile oil of the present invention (MOLA oil) is yellowish supernatant liquid, and relative density (20) is 0.9572g/ml, index of refraction 1.51.Must not be lower than 55% through gas chromatography mass spectrometry chromatography main component thymol and carvacrol content, meet the requirement of two kind new medicines.Qualitative and the relative quantification research of crude drug volatile oil component, known component is a thymol (Thymol) 58.131%, carvacrol (Cavacrol) 11.946%, cymol (P-Cymene) 10.274%, (γ-Terpinene) 5.733% for γ-Song Youxi, acetyl thymol (Thymol Acetate) 2.626%, (β-Myrcene) 1.832% for β-myrcene, a-humulene (a-Humulene) 1.638%, limonene (Limonene) 1.564%, trans-caryophyllene (trans-Caryophyllene) 0.970%, a-terpineol (a-Terpinene) 0.718%, Borneolum Syntheticum (Borneol) 0.514%, acetyl carvacrol (CavacrolAcetate) 0.384%, (β-Pinene) 0.317% for nopinene, a-pinene (a-Pinene) 0.256%, known component adds up to 96.5%, and volatile oil component is stable, place one-year age, each main component and relative scale thereof do not have significant change.
The preparation method of granular preparation of the present invention (not drawing granule): get Herba Moslae volatile oil 20-40 weight portion, add equivalent 85-95% ethanol dilution; Beta-schardinger dextrin-13-15 doubly measures, and it is miscible to add 1500ml hot water, and 30-45 ℃ of insulation mixes stirs 3-8min, by colloid mill enclose 2-4 time, refrigerator was placed more than 8-14 hour, sucking filtration, clathrate 30-45 ℃ forced air drying 4-8 hour, sieve, add dextrin to the 800-1000 weight portion, add magnesium stearate 1-3 weight portion, aspartame 1-3 weight portion, mixing, dry-pressing is granulated, granulate is made granule, and packing promptly.
The method of quality control of granular preparation of the present invention comprises following discriminating and/or assay:
Assay: the preparation of reference substance solution, precision takes by weighing thymol reference substance 8mg and thymol reference substance 4mg, puts in the 100ml measuring bottle, adds methanol to scale, shake up, precision is measured above-mentioned solution 2.5ml, puts in the 10ml measuring bottle, adds methanol to scale, shake up, that is, contain thymol 20 μ g among every ml, contain carvacrol 10 μ g.The content under the content uniformity item is got in the preparation of need testing solution, and porphyrize is got 0.12g, the accurate title, decide, and puts in the tool plug conical flask, and precision adds methanol 20ml, close plug, claim to decide weight, supersound process 30 minutes is put cold, claim to decide weight, supply the weight that subtracts mistake, shake up with methanol, filter, precision is measured subsequent filtrate 2ml, puts in the 10ml measuring bottle, add methanol and be diluted to scale, shake up, promptly.Assay method is measured according to high performance liquid chromatography (2000 editions one appendix VI D of Chinese Pharmacopoeia).Chromatographic condition and system suitability test are filler with octadecylsilane chemically bonded silica; 50-70: 50-30: 1-3 methanol-water-glacial acetic acid is a mobile phase; The detection wavelength is 276nm.Number of theoretical plate calculates by the thymol peak should be not less than 10000, calculates by the carvacrol peak and should be not less than 15000.Algoscopy, accurate respectively reference substance solution and each 20 μ l of need testing solution of drawing inject chromatograph of liquid, measure, promptly.Every g granule contains thymol (C
10H
14O) and carvacrol (C
10H
14O) total amount must not be less than 15mg.
Differentiate: by not drawing particle content measuring method, high-efficient liquid phase chromatogram has corresponding absworption peak in the retention time identical with the carvacrol reference substance with thymol.
Pharmaceutical preparation of the present invention and middle removing dampness, induce sweat and bring down a fever.Cure mainly the diarrhoea that diarrhoea, stomachache, fever with aversion to cold, head-body-pain and rotavirus or some bacterial infection cause.
Of the present inventionly do not draw particulate pharmacodynamic study result to show, do not draw granule to have following function:
1. do not draw particulate antivirus action: 12.5～25nl/ml not draw granule can suppress children's's rotavirus RDTA-I type (Wa strain) growth and breeding on African green monkey kidney cell.
2. do not draw particulate antibacterium effect: do not draw granule can reduce Salmonella enteritidis abdominal cavity infection mortality of mice (P＜0.05); Can reduce the content (P＜0.05～0.01) of pathogenic bacterium in the mice stool of Salmonella enteritidis peroral infection; External, to common pathogens such as Escherichia coli, Shiga bacillus, proteus vulgaris, staphylococcus aureus, staphylococcus epidermidiss, stronger inhibition growth effect is arranged, and test is with in the 44 strain bacterial strains, and minimum inhibitory concentration≤1ul oil/ml accounts for 79.5%.
3. do not draw particulate anti-diarrhea effect: loose stool rate, diarrhea rate, diarrhoea index and the diarrhoea degree (P＜0.05～0.001) that reduce the diarrhoea model mice due to the 100% Folium Sennae decoct significantly.
4. do not draw the influence of granule to gastrointestinal function: this medicine can reduce normally and hyperfunction mouse small intestine propulsion functions (P＜0.05～0.001) due to the neostigmine, prolong defecation time, reduce defecation quantity (P＜0.05～0.001), slow down gastric emptying motion (P＜0.01～0.001), intestinal smooth muscle excitement due to the reduction stomachial secretion function (P＜0.001), stripped intestinal smooth muscle spontaneous vasomotion of inhibition normal guinea pig and acetylcholine.
5. do not draw particulate analgesic effect: do not draw granule to reduce very significantly by the mouse writhing number due to 0.7% acetic acid (all P＜0.001), suppression ratio reaches 51.3～55.7%.
6. do not draw particulate antiinflammatory action: do not draw granule to suppress significantly by dimethylbenzene induced mice ear swelling (P＜0.01～0.001), suppress the vascular permeability (P＜0.05～0.001) that histamine causes significantly, suppress abdominal cavity leukoplania (P＜0.05～0.001) due to the sodium carboxymethyl cellulose.
7. do not draw particulate antipyretic effect: do not draw granule that saccharomycetic rat temperature is raise and endotoxin due to the rabbit body temperature rising inhibitory action (P＜0.05～0.001) is significantly all arranged.
Following experimental example is used to further specify the present invention.
Experimental example:Do not draw granule to suppress the effect of children's's rotavirus ROTA-I type (Wa strain)
Materials and methods:
1. medicine: be subjected to reagent not draw granule, MOLA oil is directly used in test, and is faint yellow, proportion 0.95726g/ml, and 16mg oil/g crude drug, lot number: 20000601, Beijing big tree institute of Pharmaceutical Research provides.Getting the 0.5ml MOLA oil adds the 0.5ml tween 80 (Shanghai the 18 pharmaceutical factory produces, lot number: add the dilution of 4ml growth-promoting media after 960520) being mixed.It is standby to put 4 ℃ of refrigerators.
Contrast medicine GEGEN QINLIAN DIWAN, the Liuzhou city pharmaceutical factory of traditional Chinese medicine produces, and accurate word (1991) is defended No. 017004 in osmanthus, lot number: 990721.Get 10 milliliters of dissolvings of 1g medicine adding distil water, 2000 rev/mins, centrifugal 10 minutes, get the supernatant water-bath and boil sterilization in 10 minutes, put 4 ℃ of refrigerators and preserve.
The above-mentioned medicinal preceding pH=7.2 that transfers
2. cell: African green monkey kidney (Vero) passage cell, the cell that grows up to monolayer is individual cells through the digestion of trypsinization liquid, is made into 60,000 cell/ml with growth-promoting media, inserts the cell pipe, the 1ml/ pipe.
3. viral: children's's rotavirus ROTA-1 type (Wa strain), TCID
50Be 10
-7, provide by Virology Inst., China Academy of Preventive Medicine Sciences, during test with 100 TCID
500.1ml.Before the Wa strain is used, add 37 ℃ of water-baths of 0.05ml pancreatin (200ug/ml) 30 minutes with 1ml virus liquid.
4. reagent: Eagles MEM dry powder, U.S. GIBCO product.Hyclone, the Tianjin Blood Research Institute is produced; Lot number: 9925.L-glutaminate, Sigma import packing, lot number: 911015.Penicillin, streptomycin.
The reagent preparation:
Growth-promoting media: Eagles adds 1% mycillin, 1% glutamine, 10% calf serum, with preceding accent PH=7.4.
Keep liquid: Eagles adds 1% mycillin, 1% glutamine, and 200ug/ml pancreatin (final concentration is 2ug/ml) is with preceding accent PH=7.4.
Cell dissociation buffer: 0.25% pancreatin, with no calcium magnesium phosphate buffer preparation.
5. test articles for use and instrument:
Culture bottle, test tube, measuring pipette, incubator, refrigerator, baking box, water bath, low temperature refrigerator, liquid nitrogen container etc. are homemade.
6. test method:
(1) MOLA oil, GEGEN QINLIAN DIWAN and tween 80 are to the toxic action of Vero cell: will dilute good medicine, tween 80 inserts and grows up in the test tube of cell monolayer, puts 37 ℃ of incubators and cultivates, every day, observation of cell changed.
(2) medicine is to the direct killing effect of virus: put 37 ℃ of incubators 1 hour after will diluting good medicine and viral mixed in equal amounts.Access grows up in the test tube of cell monolayer then, and every pipe 0.2ml is put 37 ℃ of absorption 1 hour.Keep liquid 1ml with keeping to add after liquid is washed twice.Put 37 ℃ of incubators and cultivate, observation of cell pathological changes every day (CPE), when virus control occurs ++ ++ the time, termination test.
(3) medicine inhibitory action that virus is bred in cell: the virus that will handle inserts in the cell pipe, every pipe 0.1ml is put 37 ℃ of absorption 1 hour, with keep liquid wash after twice add respectively contain different pharmaceutical concentration keep liquid 1ml, put 37 ℃ of cultivations, every day the observation of cell pathological changes, when the virus control pipe occurs ++ ++ the time, termination test.
Annotate: cytopathy :+25%, ++ 50%, +++75%, ++ ++ 100%.
More than medicine contrast, virus control, cell contrast are established in test simultaneously, and every test group 4 solencytes repeat to do experiment 2 times.
The result:
1. MOLA oil the results are shown in Table 1 to the toxicity test of Vero cell.
Table 1 MOLA oil is to the toxicity test result of Vero cell
Group drug level/ml cell pipe is counted cytotoxicity pipe number
100nl 4 4
50nl 4 0
MOLA oil
25nl 4 0
12.5nl 4 0
5mg 4 4
GEGEN QINLIAN DIWAN 2.5mg 40
1.25mg 4 0
200nl 4 2
Tween 80
100nl 4 0
Vero contrasts 40
4 concentration MOLA oil are inserted the cell pipe respectively, and final concentration is 100,50,25,12.5nl/ml.Each concentration 4 pipe was observed 7 days.Pair cell was toxic when MOLA oil concentration was 100nl/ml as a result, and concentration is 50,25, and pair cell does not have toxicity during 12.5nl/ml; Pair cell is toxic during GEGEN QINLIAN DIWAN 5mg/ml, and pair cell does not have toxicity when 2.5mg/ml and 1.25mg/ml; Pair cell does not have toxicity during tween 80 100nl/ml.
2. MOLA oil is to the direct killing effect result of the test of virus: the results are shown in Table 2.
Table 2 MOLA oil is to the direct killing effect result of the test of virus
The positive pipe of group drug level/ml inoculated tube number number negative tube number
50nl 8 8 0
The MOLA oil group
25nl 8 8 0
10mg 8 8 0
The GEGEN QINLIAN DIWAN group
5mg 8 8 0
100nl 8 8 0
The tween 80 group
50nl 8 8 0
Wa strain matched group 880
Cell matched group 808
It is as shown in the table: each administration group of MOLA oil does not have direct deactivation to children's's rotavirus ROTA-1 type.
MOLA oil to virus in intracellular breeding inhibitory action result of the test: the results are shown in Table 3.
Table 3: MOLA oil to virus in intracellular breeding inhibitory action result of the test:
Group drug level/positive the pipe of ml pipe number negative tube
25nl 8 0 8
12.5nl 8 0 8
The MOLA oil group
6.25nl 8 2 6
3.12nl 8 8 0
2.5mg 8 8 0
The GEGEN QINLIAN DIWAN group
1.25mg 8 8 0
100nl 8 8 0
The tween 80 group
50nl 8 8 0
The Wa strain contrasts 880
Cell contrasts 808
It is as shown in the table, when MOLA oil concentration is 25nl/ml, 12.5nl/ml, can suppress virus fully in intracellular breeding, when concentration is 6.25nl/ml, virus is bred certain inhibitory action in cell, when concentration is 3.1nl/ml virus being bred in cell does not have inhibitory action.GEGEN QINLIAN DIWAN, each concentration group of tween 80 is bred in cell virus does not have inhibitory action.
Embodiment 1:Get Herba Moslae volatile oil 33.3g, add equivalent 95% ethanol dilution; Beta-schardinger dextrin-500g, it is miscible to add 1500ml hot water, and 40 ℃ of insulations mix stirs 5min, by colloid mill enclose 3 times, refrigerator was placed more than 12 hours, sucking filtration, 80 mesh sieves are crossed in 40 ℃ of forced air dryings of clathrate 6 hours, add dextrin to 996g, add magnesium stearate 2g, aspartame 2g, mixing, dry-pressing is granulated, 20-80 order granulate is made the 1000g granule, and packing promptly.Every packing 1g.3 times on the one, one time 1 bag.
Embodiment 2:The method of quality control of granular preparation of the present invention
Assay: the preparation of reference substance solution, precision takes by weighing thymol reference substance 8mg and thymol reference substance 4mg, puts in the 100ml measuring bottle, adds methanol to scale, shake up, precision is measured above-mentioned solution 2.5ml, puts in the 10ml measuring bottle, adds methanol to scale, shake up, that is, contain thymol 20 μ g among every ml, contain carvacrol 10 μ g.The content under the content uniformity item is got in the preparation of need testing solution, and porphyrize is got 0.12g, the accurate title, decide, and puts in the tool plug conical flask, and precision adds methanol 20ml, close plug, claim to decide weight, supersound process 30 minutes is put cold, claim to decide weight, supply the weight that subtracts mistake, shake up with methanol, filter, precision is measured subsequent filtrate 2ml, puts in the 10ml measuring bottle, add methanol and be diluted to scale, shake up, promptly.Assay method is measured according to high performance liquid chromatography (2000 editions one appendix VI D of Chinese Pharmacopoeia).Chromatographic condition and system suitability test are filler with octadecylsilane chemically bonded silica; 60: 40: 2 methanol-water-glacial acetic acid are mobile phase; The detection wavelength is 276nm.Number of theoretical plate calculates by the thymol peak should be not less than 10000, calculates by the carvacrol peak and should be not less than 15000.Algoscopy, accurate respectively reference substance solution and each 20 μ l of need testing solution of drawing inject chromatograph of liquid, measure, promptly.Every g granule contains thymol (C
10H
14O) and carvacrol (C
10H
14O) total amount must not be less than 15mg.
Differentiate: by not drawing particle content measuring method, high-efficient liquid phase chromatogram has corresponding absworption peak in the retention time identical with the carvacrol reference substance with thymol.
Claims (4)
1, a kind of Herba Moslae volatile oil granular preparation is characterized in that said preparation made by following method:
Get Herba Moslae volatile oil 20-40 weight portion, add equivalent 85-95% ethanol dilution; Beta-schardinger dextrin-13-15 doubly measures, and it is miscible to add 1500ml hot water, and 30-45 ℃ of insulation mixes stirs 3-8min, by colloid mill enclose 2-4 time, refrigerator was placed more than 8-14 hour, sucking filtration, clathrate 30-45 ℃ forced air drying 4-8 hour, sieve, add dextrin to the 800-1000 weight portion, add magnesium stearate 1-3 weight portion, aspartame 1-3 weight portion, mixing, dry-pressing is granulated, granulate is made granule, and packing promptly.
2, a kind of Herba Moslae volatile oil granular preparation as claimed in claim 1 is characterized in that said preparation made by following method:
Get Herba Moslae volatile oil 33.3g, add equivalent 95% ethanol dilution; Beta-schardinger dextrin-500g, it is miscible to add 1500ml hot water, and 40 ℃ of insulations mix stirs 5min, by colloid mill enclose 3 times, refrigerator was placed more than 12 hours, sucking filtration, 80 mesh sieves are crossed in 40 ℃ of forced air dryings of clathrate 6 hours, add dextrin to 996g, add magnesium stearate 2g, aspartame 2g, mixing, dry-pressing is granulated, 20-80 order granulate is made the 1000g granule, and packing promptly.
3, the method for quality control of preparation as claimed in claim 1 or 2 is characterized in that this method of quality control contains following discriminating and/or assay:
Assay: the preparation of reference substance solution, precision takes by weighing thymol reference substance 8mg and thymol reference substance 4mg, puts in the 100ml measuring bottle, adds methanol to scale, shake up, precision is measured above-mentioned solution 2.5ml, puts in the 10ml measuring bottle, adds methanol to scale, shake up, that is, contain thymol 20 μ g among every ml, contain carvacrol 10 μ g; The content under the content uniformity item is got in the preparation of need testing solution, and porphyrize is got 0.12g, the accurate title, decide, and puts in the tool plug conical flask, and precision adds methanol 20ml, close plug, claim to decide weight, supersound process 30 minutes is put cold, claim to decide weight, supply the weight that subtracts mistake, shake up with methanol, filter, precision is measured subsequent filtrate 2ml, puts in the 10ml measuring bottle, add methanol and be diluted to scale, shake up, promptly; Assay method is according to high effective liquid chromatography for measuring; Chromatographic condition and system suitability test are filler with octadecylsilane chemically bonded silica; 50-70: 50-30: 1-3 methanol-water-glacial acetic acid is a mobile phase; The detection wavelength is 276nm; Number of theoretical plate calculates by the thymol peak should be not less than 10000, calculates by the carvacrol peak and should be not less than 15000; Algoscopy, accurate respectively reference substance solution and each 20 μ l of need testing solution of drawing inject chromatograph of liquid, measure, promptly; The total amount that every g granule contains thymol and carvacrol must not be less than 15mg;
Differentiate: by not drawing particle content measuring method, high-efficient liquid phase chromatogram has corresponding absworption peak in the retention time identical with the carvacrol reference substance with thymol.
4, method of quality control as claimed in claim 3 is characterized in that in this method with 60: 40: 2 methanol-water-glacial acetic acid being mobile phase.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102451228A (en) * | 2010-10-26 | 2012-05-16 | 中国医学科学院药用植物研究所 | Preparation method and application of antibacterial and antiviral elsholtzia densa benth volatile oil |
| CN103146484A (en) * | 2013-02-28 | 2013-06-12 | 青海红璟天生物科技有限公司 | Method for fast preparing betadex embedded object of rhododendron anthopogonoide volatile oil |
| CN105960167A (en) * | 2014-01-14 | 2016-09-21 | 包装,运输及物流技术研究所(Itene) | Antimicrobial compositions for food packaging consisting of salicylaldehyde and carvacrol, thymol or their mixture |
| CN109419846A (en) * | 2017-08-31 | 2019-03-05 | 安徽天康(集团)股份有限公司 | A kind of application of Herba Moslae volatile oil in treatment norovirus infectious diarrhea |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103610853B (en) * | 2013-12-13 | 2015-03-04 | 华润三九（郴州）制药有限公司 | Angelica sinensis and ligusticum wallichii tablet for regulating menstruation and preparation method of angelica sinensis and ligusticum wallichii tablet |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102451228A (en) * | 2010-10-26 | 2012-05-16 | 中国医学科学院药用植物研究所 | Preparation method and application of antibacterial and antiviral elsholtzia densa benth volatile oil |
| CN103146484A (en) * | 2013-02-28 | 2013-06-12 | 青海红璟天生物科技有限公司 | Method for fast preparing betadex embedded object of rhododendron anthopogonoide volatile oil |
| CN105960167A (en) * | 2014-01-14 | 2016-09-21 | 包装,运输及物流技术研究所(Itene) | Antimicrobial compositions for food packaging consisting of salicylaldehyde and carvacrol, thymol or their mixture |
| CN109419846A (en) * | 2017-08-31 | 2019-03-05 | 安徽天康(集团)股份有限公司 | A kind of application of Herba Moslae volatile oil in treatment norovirus infectious diarrhea |
| CN109419846B (en) * | 2017-08-31 | 2021-07-16 | 安徽天康(集团)股份有限公司 | Application of herba Moslae volatile oil in treating norovirus infectious diarrhea |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20060405 Termination date: 20191209 |
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| CF01 | Termination of patent right due to non-payment of annual fee |