CN109419846B - Application of herba Moslae volatile oil in treating norovirus infectious diarrhea - Google Patents
Application of herba Moslae volatile oil in treating norovirus infectious diarrhea Download PDFInfo
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- CN109419846B CN109419846B CN201710770806.2A CN201710770806A CN109419846B CN 109419846 B CN109419846 B CN 109419846B CN 201710770806 A CN201710770806 A CN 201710770806A CN 109419846 B CN109419846 B CN 109419846B
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- volatile oil
- elsholtzia
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/53—Lamiaceae or Labiatae (Mint family), e.g. thyme, rosemary or lavender
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/045—Hydroxy compounds, e.g. alcohols; Salts thereof, e.g. alcoholates
- A61K31/05—Phenols
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/48—Preparations in capsules, e.g. of gelatin, of chocolate
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
Abstract
The invention discloses application of elsholtzia volatile oil in treating norovirus infectious diarrhea, wherein the total amount of thymol and carvacrol in the elsholtzia volatile oil is not less than 60%, the elsholtzia volatile oil has a good curative effect on norovirus infectious diarrhea, and the elsholtzia volatile oil can recover to be normal after being taken for 2 days.
Description
Technical Field
The invention relates to an application of elsholtzia volatile oil in preparing a medicament for treating norovirus infectious diarrhea.
Background
The norovirus infectious diarrhea is diarrhea caused by norovirus, has the characteristics of quick onset, high transmission speed, wide range of involvement and the like, and is a main cause of outbreak of nonbacterial diarrhea. Norovirus has strong infectivity, is mainly transmitted through intestinal tracts, can be transmitted through polluted water sources, food, articles, air and the like, and often causes collective outbreak in communities, schools, restaurants, hospitals, nursery centers, orphans, armies and the like.
Norovirus outbreaks can be seen in all months. Some studies conducted in temperate climates have shown that months in winter have a high peak in incidence. Norovirus is transmitted from person to person via the fecal oral route with a latency period of 1-2 days. In addition, infection transmission pathways include airborne droplets of vomit containing viral particles, contaminants, or ingestion of contaminated food and water. The transmission of the virus requires a small amount of virus infection.
Given the various modes of norovirus transmission (including contact with environmental surfaces, airborne nebulization of vomit, and human-to-human contact), outbreaks of infection are difficult to control.
For norovirus infectious diarrhea, methods of warming and resolving cold-dampness and clearing and resolving damp-heat are respectively adopted according to the difference of cold and heat. For the patients with cold-dampness exuberance, diarrhea is clear and thin, even like watery, abdominal pain and borborygmus, or fever, headache, soreness of limbs, white or white greasy tongue coating, soft and slow pulse, the treatment should be mainly cold dispelling and dampness resolving, and it can be modified with Huoxiang Zhengqi san; for damp-heat injuries, diarrhea, abdominal pain, urgent diarrhea, yellow and brown feces, foul smell, burning sensation in anus, polydipsia due to feverish sensation, scanty and yellow urine, red tongue with yellow and greasy coating, slippery and rapid pulse or soft-superficial and rapid pulse can be treated on the basis of clearing heat and promoting diuresis, which can be modified with Gegenqinlian decoction. For exterior syndromes such as headache, fever and floating pulse, it can be added with Lonicera Japonica flos and forsythiae fructus for dispelling pathogenic wind and clearing heat, but these prescriptions have no instant effect on treatment.
Disclosure of Invention
The invention provides an application of elsholtzia volatile oil in preparing a medicament for treating norovirus infectious diarrhea.
In order to solve the problems, the invention adopts the technical scheme that the total amount of thymol and carvacrol in the elsholtzia volatile oil is not less than 60%, and preferably, the content of thymol in the elsholtzia volatile oil is 48-60%. The content of the carvacrol is 12% -20%.
The elsholtzia volatile oil is extracted by using a critical carbon dioxide fluid, and the extraction temperature is 20-65 ℃, preferably 30-40 ℃, and further preferably 35-38 ℃; the extraction pressure is 5-20 Mpa, preferably 8-10 Mpa, more preferably 8.5-8.9 Mpa, and the extraction time is 1-4 hours, preferably 1.5-3 hours, more preferably 2-2.5 hours.
The elsholtzia fine powder is prepared by cleaning elsholtzia, drying at a low temperature of 30-70 ℃, preferably at a temperature of 30-60 ℃, optimally at a temperature of 30-40 ℃, and crushing to 10-20 meshes, optimally 20 meshes; the elsholtzia volatile oil is prepared into a pharmaceutically oral capsule by adding various pharmaceutically allowable auxiliary materials.
The elsholtzia volatile oil mainly comprises the following components: the herba elsholtziae oil prepared by different methods contains different main components, and finally has great influence on the curative effect of the norovirus infectious diarrhea, and a great deal of experimental researches show that the total amount of thymol and carvacrol in the herba elsholtziae volatile oil is not less than 60 percent, so the herba elsholtziae volatile oil has good curative effect on the norovirus infectious diarrhea, and the herba elsholtziae oil can be recovered within 2 days after being taken.
The preparation method of the elsholtzia volatile oil provided by the invention is simple, the extraction pressure is low, the temperature is low, the operation condition is easy to control, no solvent residue exists, natural active ingredients and thermosensitive ingredients are not damaged, and the natural characteristics of the extract can be maintained to the maximum extent.
Detailed Description
For the purpose of enhancing understanding of the present invention, the present invention will be further described in detail with reference to the following examples, which are provided for illustration only and are not to be construed as limiting the scope of the present invention.
Example 1: preparation method of elsholtzia volatile oil
1. Preparing raw materials: cleaning herba Moslae with clear water, oven drying at 30 deg.C, and pulverizing into 20 mesh fine powder with pulverizer;
2. and (3) extraction: taking 600g of herba Moslae fine powder, extracting volatile oil with carbon dioxide fluid extraction device at 25 deg.C under 8.9Mpa for 2 hr to obtain 10.5ml of volatile oil, and determining the content of thymol (C10H 14O) to be 48.71%; the content of carvacrol (C10H 14O) is 13.99%.
Example 2: preparation method of elsholtzia volatile oil
1. Preparing raw materials: cleaning herba Moslae with clear water, oven drying at 40 deg.C, and pulverizing into 20 mesh fine powder with pulverizer;
2. and (3) extraction: taking 600g of herba Moslae fine powder, extracting volatile oil with carbon dioxide fluid extraction device at 39 deg.C under 8.7Mpa for 2 hr to obtain 9.7ml of volatile oil, and determining that the content of thymol (C10H 14O) is 50.26%; the content of carvacrol (C10H 14O) is 16.53%.
Example 3: preparation method of elsholtzia volatile oil
1. Preparing raw materials: cleaning herba Moslae with clear water, oven drying at 30 deg.C, and pulverizing into 20 mesh fine powder with pulverizer;
2. and (3) extraction: taking 600g of herba Moslae fine powder, extracting volatile oil with carbon dioxide fluid extraction device at 28 deg.C under 9.2Mpa for 2.5 hr to obtain 11.8ml of volatile oil, and measuring to obtain thymol (C10H 14O) content of 59.07%; the content of carvacrol (C10H 14O) is 19.75%.
Example 4: preparation method of elsholtzia volatile oil
1. Preparing raw materials: cleaning herba Moslae with clear water, oven drying at 30 deg.C, and pulverizing into 20 mesh fine powder with pulverizer;
2. and (3) extraction: taking 600g of herba Moslae fine powder, extracting volatile oil with carbon dioxide fluid extraction device at 21 deg.C under 8.6Mpa for 2 hr to obtain 10.7ml of volatile oil, and determining the content of thymol (C10H 14O) to be 53.28%; the content of carvacrol (C10H 14O) is 17.43%.
Example 5: preparation method of elsholtzia volatile oil
1. Preparing raw materials: cleaning herba Moslae with clear water, oven drying at 30 deg.C, and pulverizing into 20 mesh fine powder with pulverizer;
2. and (3) extraction: taking 600g of herba Moslae fine powder, extracting volatile oil with carbon dioxide fluid extraction device at 36 deg.C under 9.5Mpa for 2.5 hr to obtain 11.5ml of volatile oil, and determining that the content of thymol (C10H 14O) is 51.90%; the content of carvacrol (C10H 14O) is 16.92%.
Example 6: preparation method of elsholtzia volatile oil
1. Preparing raw materials: cleaning herba Moslae with clear water, oven drying at 30 deg.C, and pulverizing into 20 mesh fine powder with pulverizer;
2. and (3) extraction: taking 600g of herba Moslae fine powder, extracting volatile oil with carbon dioxide fluid extraction device at 40 deg.C under 8.5Mpa for 2.5 hr to obtain 10.4ml of volatile oil, and measuring to obtain 49.33% of thymol (C10H 14O); the content of carvacrol (C10H 14O) is 14.49%.
Preparation of Elsholtzia stauntoni oil capsule 10ml of each volatile oil prepared in the above examples 1-6 is added with 20ml of ethanol, and the mixture is stirred to obtain volatile oil solution; taking 120g of beta-cyclodextrin, adding 360ml of water, heating for dissolving, slowly adding the volatile oil solution under the condition of heat preservation and stirring at 40 ℃, preserving heat and stirring for 5 minutes, carrying out inclusion by a colloid mill for 3 times, refrigerating for more than 12 hours, carrying out suction filtration, carrying out forced air drying on the inclusion at 40 ℃ for 6 hours, sieving, adding 0.4g of magnesium stearate, mixing uniformly, and encapsulating to obtain the finished product.
Clinical cases:
chen is old, male, 11 years old. Vomiting 5 times before 2 days due to eating unwashed melons and fruits, nausea, abdominal pain, 3 times of defecation with thin water and no mucus with purulent blood. Stool routine microscopic examination (WBC): 11.85X 109/L, no RBC, no special findings on blood routine examination. Norovirus was detected in vomit. The elsholtzia oil capsules prepared in example 1 of the present invention were taken 3 times a day, 1 capsule at a time. After taking for 1 day, the symptoms are obviously relieved; after being taken for 2 days, the medicine is completely recovered to be normal, and no adverse reaction is found.
Wangzhi, female, 5 years old. Vomiting and abdominal pain occur after lunch, and the stool is thin water. Norovirus was detected in vomit by hospitalization. The elsholtzia oil capsules prepared in example 3 of the present invention were taken 3 times a day, 1 capsule at a time. After the medicine is taken for the 1 st time in the evening on the treatment day, the vomiting occurs the next day, the abdominal pain symptom disappears, the normal state is recovered, and no adverse reaction is found.
The above description is only for the purpose of illustrating the preferred embodiments of the present invention and is not to be construed as limiting the invention, and any modifications, equivalents, improvements and the like that fall within the spirit and principle of the present invention are intended to be included therein.
Claims (5)
1. An application of herba Moslae volatile oil in preparing medicine for treating norovirus infectious diarrhea is characterized in that the total amount of thymol and carvacrol in the herba Moslae volatile oil is not less than 60%; wherein, the content of thymol in the elsholtzia volatile oil is 48 to 60 percent, and the content of carvacrol is 12 to 20 percent;
the elsholtzia volatile oil is obtained by extracting fine elsholtzia powder by using a critical carbon dioxide fluid, wherein the extraction temperature is 20-65 ℃, the extraction pressure is 5-20 Mpa, and the extraction time is 1-4 hours.
2. The application of claim 1, wherein the extraction temperature is 30-40 ℃, the extraction pressure is 8-10 MPa, and the extraction time is 1.5-3 hours.
3. The application of claim 1, wherein the extraction temperature is 35-38 ℃, the extraction pressure is 8.5-8.9 MPa, and the extraction time is 2-2.5 hours.
4. The application of claim 1, wherein the fine powder of Elsholtzia stauntoni is prepared by washing Elsholtzia stauntoni, drying at 30-70 deg.C, and pulverizing to 10-20 mesh.
5. The use as claimed in claim 1, wherein the elsholtzia volatile oil is formulated into pharmaceutically acceptable oral capsules by adding pharmaceutically acceptable excipients.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201710770806.2A CN109419846B (en) | 2017-08-31 | 2017-08-31 | Application of herba Moslae volatile oil in treating norovirus infectious diarrhea |
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| CN201710770806.2A CN109419846B (en) | 2017-08-31 | 2017-08-31 | Application of herba Moslae volatile oil in treating norovirus infectious diarrhea |
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| CN109419846B true CN109419846B (en) | 2021-07-16 |
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2017
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