CN1413452A - Quick reproducing method of osmund - Google Patents
Quick reproducing method of osmund Download PDFInfo
- Publication number
- CN1413452A CN1413452A CN 02133751 CN02133751A CN1413452A CN 1413452 A CN1413452 A CN 1413452A CN 02133751 CN02133751 CN 02133751 CN 02133751 A CN02133751 A CN 02133751A CN 1413452 A CN1413452 A CN 1413452A
- Authority
- CN
- China
- Prior art keywords
- days
- prothallium
- spore
- medium
- osmund
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Cultivation Of Plants (AREA)
Abstract
A fast reproduction method for osmund includes such steps as lighting the ear-shaped sporangia to obtain its sporange and spores, immersing the spores in water for 24 hours, sterilizing, flushing with aseptic water, inoculating them to culture medium, growing protonema in 3 days, growing prothalli in 30 days, growing rosette prothalli in 20 days, high-speed reproduction in the rate of 1:4 in 60 days, growing antheridium and archegonium in 30 days, combining sperm with ovum to become embryo, developing sporophyte in 30 days, transplanting in 60 days, and permanent planting.
Description
Technical field: the invention belongs to biological technical field, particularly, relate to the method for quickly breeding of the plant osmund in the biological technical field.
Technical background: osmund (Osmunda japonica Thunb.) brake (Osmunda cinnanmoneaL.var.asiatica) belongs to Osmundaceae Osmunda pteridophyte, another name common vetch dish, tiger platform and water bone dish etc.Its its fist web-like spire is edible, can be made into dried common vetch dish and be Dried Osmund (have red, blue or green two kinds), is the high-grade well sold and in short supply non-harmful product in international market.According to surveying and determination, contain protein 3.1 grams in per 100 gram fresh vegetables, carbohydrate 4.0 grams, cellulose 3.8 grams, 0.25 milligram of VB2,69 milligrams of VC, 1.97 milligrams of carotin; Per 100 gram dry weights contain potassium 3.12 grams, 190 milligrams of calcium, 293 milligrams in magnesium, 12.5 milligrams of iron, 8.1 milligrams in manganese, 1.8 milligrams of copper, 6.2 milligrams on zinc, nutritious, bitter is cool in nature, contains steroids such as sharp leaf soil China fir sterone A, the pine of liking gram and ecdysterone because of it, and the effect of heat-clearing, detoxifcation, hemostasis, desinsection, sterilization is arranged, staphylococcus aureus, Pseudomonas aeruginosa all there are inhibitory action, cure mainly dysentery and gynaecological disease etc.Be worth because it has than large economy, adopt disorderly that disorderly to dig phenomenon very serious, cause wild resource to fall sharply.Osmund upgrades difficulty naturally, and the initial stage speed of growing is extremely slow, and this is to artificial expansion plantation and to form dried common vetch dish secondary industry very unfavorable.Up to now, the group culturation rapid propagating technology of osmund does not appear in the newspapers.
Summary of the invention: the objective of the invention is to an above-mentioned difficult problem, the method for quickly breeding of a kind of osmund is provided, make the aseptic quick breeding of osmund become possibility, can the anniversary carry out seedling production at the prior art existence.To achieve these goals, the invention provides following technical scheme:
The method for quickly breeding of a kind of osmund, get osmund spike sporangium preface and under distance 15cm illumination, obtain sporangium or spore, spore imbibition 24 hours in water, sterilization, the aseptic water washing sterilant residue, insert in the medium, left and right sides spore germination in 3 days forms protonema, begins to show prothallium in about 30 days, continues cultivation and forms the prothallium of growing thickly in about 20 days, prothallium was bred with 1: 4 reproduction speed about 60 days, spermatangium and archegonium appear in single prothallium after 30 days, seminal vesicle is in conjunction with forming blastular, and growing about 30 days is sporophyte, test tube sporophyte just portable about 60 days, but just field planting behind the transition transplanting survival.
In the said method, with spore inoculating on the 1/10MS medium, add KT 1mg/L, sugar-free, replace agar with degreasing cotton, 3 days left and right sides spores begin to sprout, and carry out mitosis and produce initial rhizoid and protonema cell, 1500 lx illuminance, irradiation 10 hours/day, 25 ℃ ± 2 ℃ of culturing room, the dark cultivation suppressed spore germination, the protonema life period is shorter, general 2-10 cell do not wait, and adds the basic element of cell division and growth hormone such as KT 1+2,4-D0.4 then on the 1/10MS medium, BA 0.5+NAA 0.5 etc., or add inhibition growth stimulator such as CCC etc., form the ligule prothallium of plump semicircle molded breadth; Form band shape prothallium when perhaps adding NAA or IBA0.5-4; On the MS-1/10MS medium, form the clearly demarcated heart-shaped prothallium of back of the body abdomen that the spill meristematic zone is arranged normally, more than the prothallium of 3 kinds of forms all form sporophyte.
The present invention's method the most particularly is to get osmund spike sporangium preface to obtain sporangium or spore under distance 15cm illumination, spore imbibition 24 hours in water, sterilization, the aseptic water washing sterilant residue, be seeded on the 1/10MS medium, add KT 1mg/L, sugar-free replaces agar with degreasing cotton, 3 days left and right sides spores begin to sprout, carry out mitosis and produce initial rhizoid and protonema cell, the protonema life period is shorter, and general 2-10 cell do not wait, 1500 lx illuminance, irradiation 10 hours/day, 25 ℃ ± 2 ℃ of culturing room, the dark cultivation suppressed spore germination, on the 1/10MS medium, add the basic element of cell division and growth hormone such as KT1+2 then, 4-D0.4, BA 0.5+NAA 0.5 etc., or add inhibition growth stimulator such as CCC etc., form the ligule prothallium of plump semicircle molded breadth; Form band shape prothallium when perhaps adding NAA or IBA0.5-4; On the MS-1/10MS medium, form the clearly demarcated heart-shaped prothallium of back of the body abdomen that the spill meristematic zone is arranged normally, began to show prothallium in about 30 days, continue cultivation and formed the prothallium of growing thickly in about 20 days, prothallium was bred with 1: 4 reproduction speed about 60 days, spermatangium and archegonium appear in single prothallium after 30 days, smart ovum is in conjunction with forming embryo, and about about 30 days grow is sporophyte, test tube sporophyte just portable about 60 days, but just field planting after transition survives.
The proposition of said method of the present invention is based on following principle:
1, the adult strain spore amount of the every strain of osmund reaches tens million of (can educate the leaf size and be about 7 * 19cm, spore count is about 5 * 10), so spore is the optimal material of aseptic quick breeding.
2, in a single day the clone of osmund is set up, and fast 6 months breeding cycles, growth rate was 1: 4 in 60 days.
3, the spore subcircular of osmund is rich in chlorophyll, and no perisporium, outer wall are thinner, so spore presents green.Spore does not have resting stage, and the spore water content is higher, so the spore life-span is extremely short, breaks away from spore 1-2 days of the fern leaf just inactivations.Following bottle of interior aseptic storage of preserving moisture of room temperature, the spore life-span can extend to more than two months.
4, condition of culture: spore inoculating is added KT 1mg/L on the 1/10MS medium, sugar-free replaces agar with degreasing cotton, and 3 days left and right sides spores just carry out mitosis and produce initial rhizoid and protonema cell, and this indicates spore germination.1500 lx illuminance, irradiation 10 hours/day.25 ℃ ± 2 ℃ of culturing room.The dark cultivation suppressed spore germination.The protonema life period is shorter, and general 2-10 cell do not wait, and becomes with condition of culture.Version and growth type at the natural world prothallium are criteria for classifications, but under the aseptic culture condition, the prothallium form is plastic, and main forms has three kinds.The additional basic element of cell division and growth hormone (as KT 1+2,4-D0.4, BA 0.5+NAA 0.5 etc.) on the 1/10MS medium, or add when suppressing growth stimulator (as CCC etc.), the ligule prothallium of plump semicircle molded breadth formed; When adding NAA or IBA0.5-4, form band shape prothallium; On the MS-1/10MS medium, form the clearly demarcated heart-shaped prothallium of back of the body abdomen that the spill meristematic zone is arranged normally.More than the prothallium of 3 kinds of forms all can form sporophyte.Remove the 1/10MS medium+sucrose 5% of trace element, favourable sporophyte forms.
Embodiment:
Further essentiality content of the present invention is described below in conjunction with embodiment, but content of the present invention is not limited thereto.
Embodiment 1:
Get osmund spike sporangium preface and (adopt brake February, adopt the sporangium preface of osmund mid-March), illumination (apart from 15cm) down sporangium or spore, spore imbibition 24 hours in water, sterilization, the aseptic water washing sterilant residue is seeded on the 1/10MS medium, add KT 1mg/L, sugar-free replaces agar with degreasing cotton, 3 days left and right sides spores begin to sprout, carry out mitosis and produce initial rhizoid and protonema cell, the protonema life period is shorter, and general 2-10 cell do not wait, 1500 lx illuminance, irradiation 10 hours/day, 25 ℃ ± 2 ℃ of culturing room, the dark cultivation suppressed spore germination, on the 1/10MS medium, add the basic element of cell division and growth hormone such as KT 1+2 then, 4-D0.4, BA 0.5+NAA 0.5 etc., or add inhibition growth stimulator such as CCC etc., form the ligule prothallium of plump semicircle molded breadth; Form band shape prothallium when perhaps adding NAA or IBA0.5-4; On the MS-1/10MS medium, form the clearly demarcated heart-shaped prothallium of back of the body abdomen that the spill meristematic zone is arranged normally, began to show prothallium in about 30 days, continue cultivation and formed the prothallium of growing thickly in about 20 days, prothallium was bred with 1: 4 reproduction speed about 60 days, spermatangium and archegonium appear in single prothallium after 30 days, smart ovum is in conjunction with forming embryo, and about about 30 days grow is sporophyte, test tube sporophyte just portable about 60 days, but just field planting after transition survives.
The characteristic of osmund: plant height 60-100 centimetre, the root-like stock tubbiness, upright or tiltedly living, the top leafage gives birth to.The trophophyll petiole is long, normal tool rust fine hair, two times pinniform drastic cracks; Sporophyll is little, and Dan Sheng is close by sepia fine hair, the close living brown sporangium in the back side.Breed available sporogenesis or division propagation.The property happiness dark and damp, be afraid of drought, non-refractory, shading to promote germination and growth.Acid resistance is strong, and yield and quality improves greatly in being rich in organic rich soil.
Cultivation method:
1, plant the time: spring and autumn all can, how May early and middle ten days carry out, it is fast to live, the survival rate height.2, moist fertile low-lying land is selected in whole ground bedding, and 667 square metres apply base manure 2000-3000 kilogram, plough deeply post-trenching, flat bedding cultivation, can with interplantings such as melon, beans or corn, in order to shading.Also can dig the cave and plant in the deserted mountain that has abundant water resources, mountain region.3, method for planting: (but present wild resource seldom to take root-like stock under the perennial wild common vetch vegetable plot, should limit and excavate), stem slightly is advisable with the 8-10 millimeter, balled transplanting as far as possible, 667 square metres of 5000-7000 strains of can planting, make the phyllopodium top upwards when planting, after earthing 4-6 centimetre, water immediately.4, field management: the back intertill and clean tillage in time of planting, shade and water, begin every year to gather and strengthened rich water quality management in preceding about 15 days.5, timely collecting: plant and generally do not gather then, looked its upgrowth situation in 1 year can suitably gather, mean temperature of air is more than 10 ℃ the time, and be unearthed back 6-10 days of spire is carried out first batch in good time and gathered; After 7-10 days, when second recommend spire and be unearthed after, seedling can be gathered for high about 20 centimetres.Gather every year and two recommend, later spire keeps, and makes nutrient accumulation in root-like stock, in order to spire growth in next year.Long when gathering to gather about 20 centimetres, and the hook-shaped spire that does not stretch in top is advisable, and generally need not lancinate, in order to avoid root is injured.Common vetch dish after gathering should in time be put the frescade, as early as possible processing.
Adopt back processing:
1, classification: excise the termination and the fine hair of aging part in common vetch dish bottom and hook volume, the common vetch dish of choosing foreign material and going bad, damage by worms is pressed thickness grading, and soaks anti-aging with cold water.2, poach: the fresh vegetable put in order is put into boiled water pot respectively by thickness scalded 3-5 minute, water will flood the common vetch dish, and the very hot oven heating is well-done and mashed, up to sending fragrant, and can pull out when the top can be torn in two from base portion.3, the stranding of drying in the air: the boiling hot dish that will boil in time spreads in the ventilation airing of clean place, often stirs, and common vetch dish surface moisture is dried carry out when transferring aubergine to rubbing the first time, then continue airing to the top when dry, carry out rubbing the second time, rub with the hands while shining six or seven times, till drying to its wrinkle.Remove blackening, mashed and length simultaneously at the common vetch dish below 10 centimetres.
Claims (3)
1, the method for quickly breeding of a kind of osmund, it is characterized in that getting osmund spike sporangium preface and under distance 15cm illumination, obtain sporangium or spore, spore imbibition 24 hours in water, sterilization, the aseptic water washing sterilant residue, insert in the medium, left and right sides spore germination in 3 days forms protonema, begins to show prothallium in about 30 days, continues cultivation and forms the prothallium of growing thickly in about 20 days, prothallium was bred with 1: 4 reproduction speed about 60 days, spermatangium and archegonium appear in single prothallium after 30 days, smart ovum is in conjunction with forming embryo, and about about 30 days grow is sporophyte, test tube sporophyte just portable about 60 days, but just field planting after transition survives.
2, method according to claim 1, it is characterized in that spore inoculating is on the 1/10MS medium, add KT 1mg/L, sugar-free, replace agar with degreasing cotton, 3 days left and right sides spores begin to sprout, and carry out mitosis and produce initial rhizoid and protonema cell, 1500 lx illuminance, irradiation 10 hours/day, 25 ℃ ± 2 ℃ of culturing room, the dark cultivation suppressed spore germination, the protonema life period is shorter, general 2-10 cell do not wait, and adds the basic element of cell division and growth hormone such as KT 1+2,4-D0.4 then on the 1/10MS medium, BA 0.5+NAA 0.5 etc., or add inhibition growth stimulator such as CCC etc., form the ligule prothallium of plump semicircle molded breadth; Form band shape prothallium when perhaps adding NAA or IBA0.5-4; On the MS-1/10MS medium, form the clearly demarcated heart-shaped prothallium of back of the body abdomen that the spill meristematic zone is arranged normally, more than the prothallium of 3 kinds of forms all form sporophyte.
3, method according to claim 1 and 2, it is characterized in that getting osmund spike sporangium preface and under distance 15cm illumination, obtain sporangium or spore, spore imbibition 24 hours in water, sterilization, the aseptic water washing sterilant residue is seeded on the 1/10MS medium, adds KT 1mg/L, sugar-free, replace agar with degreasing cotton, 3 days left and right sides spores begin to sprout, and carry out mitosis and produce initial rhizoid and protonema cell, the protonema life period is shorter, general 2-10 cell do not wait 1500 lx illuminance, irradiation 10 hours/day, 25 ℃ ± 2 ℃ of culturing room, the dark cultivation suppressed spore germination, adds the basic element of cell division and growth hormone such as KT 1+2,4-D0.4 then on the 1/10MS medium, BA 0.5+NAA 0.5 etc., or add inhibition growth stimulator such as CCC etc., form the ligule prothallium of plump semicircle molded breadth; Form band shape prothallium when perhaps adding NAA or IBA0.5-4; On the MS-1/10MS medium, form the clearly demarcated heart-shaped prothallium of back of the body abdomen that the spill meristematic zone is arranged normally, began to show prothallium in about 30 days, continue cultivation and formed the prothallium of growing thickly in about 20 days, prothallium was bred with 1: 4 reproduction speed about 60 days, spermatangium and archegonium appear in single prothallium after 30 days, smart ovum is in conjunction with forming embryo, and about about 30 days grow is sporophyte, test tube sporophyte just portable about 60 days, but just field planting after transition survives.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02133751 CN1413452A (en) | 2002-09-11 | 2002-09-11 | Quick reproducing method of osmund |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 02133751 CN1413452A (en) | 2002-09-11 | 2002-09-11 | Quick reproducing method of osmund |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1413452A true CN1413452A (en) | 2003-04-30 |
Family
ID=4747366
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 02133751 Pending CN1413452A (en) | 2002-09-11 | 2002-09-11 | Quick reproducing method of osmund |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1413452A (en) |
Cited By (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104322285A (en) * | 2014-11-03 | 2015-02-04 | 贵州省植物园 | Seedling culture method of osmunda cinnamomea |
| CN104542300A (en) * | 2015-01-28 | 2015-04-29 | 遵义师范学院 | Culture medium for each in-vitro culture stage of osmundaceae |
| CN104542301A (en) * | 2015-01-28 | 2015-04-29 | 遵义师范学院 | Method for culturing osmunda japonica by using in-vitro tissue |
| CN104686174A (en) * | 2015-03-11 | 2015-06-10 | 安庆万草千木农业科技有限公司 | Novel method for improving farmland planted selenium-rich vegetables and fruits |
| CN104686165A (en) * | 2015-03-11 | 2015-06-10 | 安庆万草千木农业科技有限公司 | New method for improving cultivation of selenium-enriched tea leaves |
| CN104686170A (en) * | 2015-03-11 | 2015-06-10 | 安庆万草千木农业科技有限公司 | Novel selenium-enriched tea cultivation method |
| CN104686167A (en) * | 2015-03-11 | 2015-06-10 | 安庆万草千木农业科技有限公司 | Method for achieving united interplanting of tea-oil trees in farmland |
| CN104756712A (en) * | 2015-04-08 | 2015-07-08 | 安庆万草千木农业科技有限公司 | Planting method for Chinese herbal medicine through improved farmland |
| CN105052463A (en) * | 2015-07-30 | 2015-11-18 | 合肥元政农林生态科技有限公司 | Osmunda japonica spore seedling growing technology |
| CN105230483A (en) * | 2015-09-22 | 2016-01-13 | 南昌大学 | Method for establishing in-vitro regeneration system of Osmunda vachellii |
| CN105248101A (en) * | 2015-10-28 | 2016-01-20 | 湖北长友现代农业股份有限公司 | Field cultivation method of spore second stage seedlings of southern osmunda japonica |
| CN106171999A (en) * | 2016-07-21 | 2016-12-07 | 深圳市仙湖植物园管理处 | Guangdong osmund can educate Spore cultivation and propagation method thereof |
| CN106718899A (en) * | 2016-12-08 | 2017-05-31 | 大连民族大学 | A kind of method of mountain region high-yield culturing common vetch dish |
| CN108651254A (en) * | 2018-05-17 | 2018-10-16 | 中国科学院东北地理与农业生态研究所 | A kind of efficient seedling from spore method of Asia plant division osmund four-part form |
| CN109601387A (en) * | 2019-01-25 | 2019-04-12 | 徐州生物工程职业技术学院 | With the Osmunda Vachellii Hook tissue culture propagation method of the GGB approach of young sporangiorus induction |
-
2002
- 2002-09-11 CN CN 02133751 patent/CN1413452A/en active Pending
Cited By (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104322285A (en) * | 2014-11-03 | 2015-02-04 | 贵州省植物园 | Seedling culture method of osmunda cinnamomea |
| CN104322285B (en) * | 2014-11-03 | 2016-04-20 | 贵州省植物园 | The seedling-cultivating method of a kind of brake |
| CN104542300B (en) * | 2015-01-28 | 2016-08-24 | 遵义师范学院 | The culture medium in osmund cultured in vitro each stage |
| CN104542300A (en) * | 2015-01-28 | 2015-04-29 | 遵义师范学院 | Culture medium for each in-vitro culture stage of osmundaceae |
| CN104542301A (en) * | 2015-01-28 | 2015-04-29 | 遵义师范学院 | Method for culturing osmunda japonica by using in-vitro tissue |
| CN104686174A (en) * | 2015-03-11 | 2015-06-10 | 安庆万草千木农业科技有限公司 | Novel method for improving farmland planted selenium-rich vegetables and fruits |
| CN104686165A (en) * | 2015-03-11 | 2015-06-10 | 安庆万草千木农业科技有限公司 | New method for improving cultivation of selenium-enriched tea leaves |
| CN104686170A (en) * | 2015-03-11 | 2015-06-10 | 安庆万草千木农业科技有限公司 | Novel selenium-enriched tea cultivation method |
| CN104686167A (en) * | 2015-03-11 | 2015-06-10 | 安庆万草千木农业科技有限公司 | Method for achieving united interplanting of tea-oil trees in farmland |
| CN104686167B (en) * | 2015-03-11 | 2018-07-17 | 九江市云山油茶科技发展有限公司 | A method of realizing the joint interplanting tea oil tree in farmland |
| CN104686174B (en) * | 2015-03-11 | 2018-05-25 | 湖南白龙园林绿化有限公司 | A kind of new method for being used to improve farmland plantation rich selenium vegetables and fruit |
| CN104686170B (en) * | 2015-03-11 | 2018-05-25 | 德兴市官山茶业有限公司 | A kind of new method for selenium-enriched tea leaf plantation |
| CN104756712B (en) * | 2015-04-08 | 2018-03-02 | 德兴市百草园生态健康养生有限公司 | A kind of implantation methods improved farmland and be used for medicinal material |
| CN104756712A (en) * | 2015-04-08 | 2015-07-08 | 安庆万草千木农业科技有限公司 | Planting method for Chinese herbal medicine through improved farmland |
| CN105052463A (en) * | 2015-07-30 | 2015-11-18 | 合肥元政农林生态科技有限公司 | Osmunda japonica spore seedling growing technology |
| CN105230483A (en) * | 2015-09-22 | 2016-01-13 | 南昌大学 | Method for establishing in-vitro regeneration system of Osmunda vachellii |
| CN105248101A (en) * | 2015-10-28 | 2016-01-20 | 湖北长友现代农业股份有限公司 | Field cultivation method of spore second stage seedlings of southern osmunda japonica |
| CN106171999A (en) * | 2016-07-21 | 2016-12-07 | 深圳市仙湖植物园管理处 | Guangdong osmund can educate Spore cultivation and propagation method thereof |
| CN106171999B (en) * | 2016-07-21 | 2018-04-20 | 深圳市仙湖植物园管理处 | The fertile Spore cultivation of Guangdong osmund and its propagation method |
| CN106718899A (en) * | 2016-12-08 | 2017-05-31 | 大连民族大学 | A kind of method of mountain region high-yield culturing common vetch dish |
| CN108651254A (en) * | 2018-05-17 | 2018-10-16 | 中国科学院东北地理与农业生态研究所 | A kind of efficient seedling from spore method of Asia plant division osmund four-part form |
| CN109601387A (en) * | 2019-01-25 | 2019-04-12 | 徐州生物工程职业技术学院 | With the Osmunda Vachellii Hook tissue culture propagation method of the GGB approach of young sporangiorus induction |
| CN109601387B (en) * | 2019-01-25 | 2021-08-24 | 徐州生物工程职业技术学院 | Tissue culture propagation method of osmunda vachellii with GGB route induced by juvenile sporocyst group |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102084774B (en) | Method for raising seedling of oil palm seed | |
| CN105230497B (en) | A kind of production method of Hainan Region white flower oil tea tissue-cultured seedling | |
| CN1413452A (en) | Quick reproducing method of osmund | |
| CN109601283B (en) | Method for improving watermelon seedling quality and efficiency | |
| CN105766619B (en) | A kind of breeding method of day lily seed | |
| CN103416304A (en) | Method for cultivating water-saving and drought-resistant rice anther | |
| CN106818472A (en) | A kind of utilization callus from stem segment cultivates the propagation method of chocho | |
| CN108513859A (en) | Application and cultural method of the waste and old white fungus stick in terms of cultivating black fungus | |
| CN104247621A (en) | Culturing and transplanting method for magnolia denudata | |
| CN113080056B (en) | Cultivation method of ginseng and Ji fruit | |
| CN106561444A (en) | Konjac cultivating method | |
| CN112753575B (en) | High-yield Cremastra appendiculata seedling cultivation method | |
| CN104719156A (en) | Method for performing in-vivo induction on vaccinium australe tetraploid | |
| CN106857258A (en) | A kind of quick breeding method for tissue culture blue with leaf pocket | |
| CN106489723A (en) | A kind of inbred line breeding method of anti-celery cabbage clubroot | |
| CN105145042B (en) | Virus-free basic potato seed soil incubation method | |
| CN106386504A (en) | Tissue culture method of Aralia Cordata Thunb seedlings | |
| CN107455255B (en) | A kind of selection of broccoli | |
| CN105144912B (en) | A kind of method that largeflower-like honeysuckle flower seed is sprouted | |
| CN114831025A (en) | Rapid induction method of konjac polyploids | |
| CN112616663A (en) | Method for greatly shortening planting period of lilium davidii var davidii and rapidly propagating seedlings | |
| CN111543278A (en) | Breeding mode of orchid seeds | |
| CN105850485A (en) | Seedling growing method of hyacinth beans | |
| CN115715525B (en) | Tissue culture and rapid propagation method of calyx parviflora | |
| CN109392681B (en) | Seedling raising method for oil palm |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |