CN1406630A - Composite enzyme reacting liquid - Google Patents
Composite enzyme reacting liquid Download PDFInfo
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- CN1406630A CN1406630A CN01130990A CN01130990A CN1406630A CN 1406630 A CN1406630 A CN 1406630A CN 01130990 A CN01130990 A CN 01130990A CN 01130990 A CN01130990 A CN 01130990A CN 1406630 A CN1406630 A CN 1406630A
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- compound enzyme
- enzyme
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Abstract
A composite enzyme reaction liquid for enzymolytic fermentation of raw materials is prepared from composite enzyme, plasmodial liquid, enzyme promoter, inorganic salt containing trace elements, distilled water and assistant. It can be used in pharmaceutical industry of Chinese medicine.
Description
Technical field
The invention belongs to the zymotechnic field, more particularly, is a kind of compositions that contains the reactant liquor of multiple compound enzyme.
Background technology
The industrial list that usually uses is planted enzyme.In the later stage in the sixties, the production of microbial lipase has had very big development, and Japan has classified one of industrial enzyme as.The seventies rises aspect seeking novel bacterial and special enzyme, increase output, improvement separate purification and assay method and immobilized enzyme and has all obtained progress.
Summary of the invention
The purpose of this invention is to provide a kind of reactant liquor that contains compound enzyme that is widely used in Chinese herbal medicine, food, beverage, health product.
Main technical schemes of the present invention: compound enzyme, paraplasm, enzyme promoter, the inorganic salt that contains trace element, distilled water and adjuvant are mixed into the compound enzyme reactant liquor.
Compound enzyme reactant liquor of the present invention constituent and content thereof (is 100 by the composition weight sum):
Compound enzyme 2～5%, paraplasm 4.5～60%, enzyme promoter 5～10%, distilled water 10～70%, inorganic salt 0.5～16%, adjuvant 3～24%.
Compound enzyme of the present invention be cellulase, pectase, glucose isomerase, amylase, lipase with 2: 2: 3: the mixed enzyme of 3: 3 weight ratios.Described cellulase, pectase, glucose isomerase, amylase, lipase all are by known method production (Zhang Shuzheng chief editor, " enzyme preparation industry ", Science Press, version in 1989), and except that cellulase, other four kinds of enzymes all can be buied on market.Described cellulase adopts the biological engineering mode to produce, and cultivates at normal temperatures and pressures.Concrete technological process: (1) strain: Trichoderma viride bacterial strain; (2) dilution of bacterial strain and mutation: in beaker, dilute the Trichoderma viride bacterial strain by the pure mineral water of 1/2 multiple, be adjusted to pH value 4-4.5, soaked 24 hours, and place under the uviol lamp cross processing 7 times, about 28 ℃ of temperature with lactic acid; (3) cultivate: add Testa oryzae 1%, Testa Tritici 0.5%, ammonium sulfate 0.2%, potassium dihydrogen phosphate 0.1%, calcium carbonate 0.1%, glucose 1%, cultivated PH4.0-4.5, temperature 23-27 ℃ 24 hours; (4) add culture medium again: Testa Tritici 0.5%, glucose 1%, ammonium sulfate 0.2%, potassium dihydrogen sulfate 0.1%, incubation time 24 hours, PH4.0-4.5, temperature 27-30 ℃; (5) in culture medium, continue to cultivate to add: peptone 0.075%, tween 0.2%, ammonium sulfate 0.03%, potassium dihydrogen sulfate 0.2%, carbamide 0.03%, calcium chloride 0.03%, magnesium sulfate 0.03%, iron sulfate 0.005%, manganese sulfate 0.016%, zinc sulfate 0.014%, cobaltous chloride 0.02%, cultivated 48 hours, PH4.0-5.0, temperature 26-29 ℃; (6) filter for the first time: get supernatant, add magnesium sulfate 0.5%, placed 24 hours; (7) filter for the second time: get supernatant; (8) centrifugal: taking precipitate (15 minutes time, rotating speed 1500 changes); (9) collecting precipitation thing, drying, pulverize, baking temperature is lower than 60 ℃, powder fineness 100 orders.
Five kinds of enzymes forming compound enzyme all are ＂ hydrolytic enzyme ＂, and different enzymes is had different catalyst mechanisms:
Described cellulase, C
1Component and C
xComponent has mutual synergism to dissolving cellulos, converts it into glucose at last.
The main source of described pectase is that mycetes such as putrid Fructus Mali pumilae, Fructus Musae, Fructus Vitis viniferae, Fructus Citri tangerinae are microorganism fungus kind, so its contained component is PE (pectinesterase).PE can make the pectinesterase hydrolysis in the Chinese medicinal herbs, generates pectic acid, forms the nutrition in the health medicine.
Described glucose isomerase claims xylose isomerase (EC5,3,1,5) again, and it can reversibly be converted into corresponding ketose with aldoses such as D-xylose, D-glucose, D-ribose.Glucose isomerase is an acidic protein, and acidic amino acid residue is more than basic amino acid in the molecule.
Described diastatic action can cut α-1,4 key and generate dextrin and reducing sugar when starch from intramolecule.
Described lipase is the enzyme of the bright fat of branch, and the fat here is meant biogenic natural oil, and the part that hydrolysis takes place is greasy ester bond.
The concrete system method of compound enzyme: five kinds of enzymes are mixed in proportion, and with shaking table its mix homogeneously are got final product.
The paraplasm that paraplasm of the present invention is normally squeezed, extracted from fruit, vegetable etc. comprises a kind of, two or more the mixture of Semen Maydis pulp, Semen Maydis powder, Radix Dauci Sativae juice, Ovum Gallus domesticus album, Sucus Mali pumilae, Semen sojae atricolor powder, Sucus Pyri, radish Juice, green vegetable juice, Succus Momordicae charantiae, Oleum Glycines, beerwort, cabbage juice, Semen Nelumbinis juice, bean sprout juice, Lac Bovis seu Bubali.Paraplasm in application process mainly as auxin.Auxin is essential to enzymatic activity, composition in these auxin relevant with the yeast glycolytic activity is called coenzyme, itself is not an enzyme, neither protein, be generally HA (NAD) and HA phosphoric acid (NADP).NAD, NADP and other coenzyme relevant with oxidoreductase play a part hydrogen donor or oxygen acceptor.It is a kind of intermediate, does not have these intermediate, and enzymolysis can't go on, very high but the main cause that hydrolysis result is bad of some enzymatic activity why that Here it is.
Enzyme promoter of the present invention comprises a kind of, two or more the mixture of yeast extract, Tween 80, Hongqu powder (red colouring agent).Enzyme promoter belongs to the inducer or the surfactant of enzyme, can increase the enzyme speed of producing, and improves enzyme digestion reaction speed.
The inorganic salt that contains trace element of the present invention comprises KH
2PO
4, K
2HPO
4, (NH
4)
2HPO
4, KNO
3, NaNO
3, MgSO
4, Na
2SO
4, ZnSO
4, CaCL
2, CaSO
4, (NH
4)
2SO
4, NH
4NO
3, KCl, FeSO
4At interior a kind of, two or more mixture.Metal ion in the inorganic salt is many-sided for the influence of enzymolysis, phosphorus is the composition that constitutes nucleic acid, phospholipid, and participate in adenosine triphosphate (ATP) and guanosine triphosphate (GTP) at all interior energy transfer reactions, time spent also is the active group composition of many enzymes, because the phosphorus amount in organic fermentation raw material often can not satisfy the enzymolysis needs, must add; Sulfur is the ingredient of sulfur-containing amino acid such as cystine in the tropina, cysteine, methionine etc., also is the ingredient of coenzyme; Potassium is present in the thalline with ionic condition, and its effect is the saturating property effect of interior plasmic colloidal state of control cell and cell membrane; Magnesium exists in the thalline with ionic condition, and the activity of plurality of enzymes is had facilitation; Calcium can play effects such as control cell permeability and acid adjustment degree with ionic condition, and the activity of plurality of enzymes such as amylase, fungal proteinase is played stable and activation; Sodium metal can promote the output of enzyme.
Adjuvant of the present invention comprises a kind of, two or more the mixture of peptone, egg albumen powder, glycerol, yeast extract, glucose, Lac regis apis, agar, lactic acid, white sugar, brown sugar, citric acid.The main effect of adjuvant is the ratio of regulating carbon and nitrogen, promotes fermentation, stablizes pH value.
Distilled water of the present invention can make according to a conventional method.
Compound enzyme reactant liquor of the present invention can be divided into 10 classes by purposes, and its composition and content (is 100 in the composition weight sum) are as follows:
1. be used for the green plants extract
(1) paraplasm 4.8%, wherein Semen Maydis pulp 1.8%, Radix Dauci Sativae juice 1%, Ovum Gallus domesticus album 2%;
(2) enzyme promoter 6.5%, wherein yeast extract 3%, Tween 80 1.5%, Hongqu powder (red colouring agent) 2%;
(3) inorganic salt 7.4%, wherein KH
2PO
40.1%, KH
2PO
40.1%, MgSO
41.5%, Na
2SO
44.5%, (NH
4)
2SO
40.7%, KCl0.5%;
(4) compound enzyme 2%;
(5) adjuvant 9.3%, wherein peptone 2%, glucose 6%, Lac regis apis 1.3%;
(6) distilled water 70%.
2. be used for antiaging agent series
(1) paraplasm is Sucus Mali pumilae 10%;
(2) enzyme promoter 10%, wherein Tween 80 5%, Hongqu powder (red colouring agent) 5%;
(3) inorganic salt 3.65%, wherein KH
2PO
40.1%, MgSO
40.25%, NH
4NO
32.5%, CuSO
40.2%, ZnSO
40.2%, FeSO
40.2%, MnSO
40.2%;
(4) compound enzyme 2%;
(5) adjuvant 9.35%, wherein brown sugar 3.6%, agar 2%, citric acid 3.75%;
(6) distilled water 65%.
3. be used for the sick blood vessel drug eluting series of anti-heart and brain
(1) paraplasm 32.6%, wherein Semen sojae atricolor powder 5%, Semen Maydis powder 5%, Sucus Pyri 10%, Sucus Mali pumilae 10%, Ovum Gallus domesticus album 2.6%;
(2) enzyme promoter is Tween 80 5%;
(3) inorganic salt 0.6%, wherein KH
2PO
40.2%, NaCl0.2%, (NH
4)
2SO
40.2%;
(4) compound enzyme 2%;
(5) adjuvant 10.8%, wherein agar 2%, citric acid 3.8%, peptone 5%;
(6) distilled water 49%;
4. be used for cancer therapy drug series
(1) paraplasm is Radix Dauci Sativae juice 10%;
(2) enzyme promoter 10%, wherein Tween 80 5%, Hongqu powder (red colouring agent) 5%;
(3) compound enzyme 4%;
(4) adjuvant 24%, wherein citric acid 20%, agar 2%, peptone 2%;
(5) inorganic salt 7%, wherein MgSO
41%, KH
2PO
41%, (NH
4)
2HPO
42%, KCl1%, NH
4NO
32%;
(6) distilled water 45%;
5. be used for difficult miscellaneous disease medicine series
(1) paraplasm 50%, wherein Sucus Pyri 20%, radish Juice 10%, green vegetable juice 10%, Succus Momordicae charantiae 10%;
(2) enzyme promoter is Tween 80 5%;
(3) inorganic salt 16%, wherein ZnSO
48%, MgSO
42%, KH
2PO
46%;
(4) compound enzyme 4%;
(5) adjuvant is a lactic acid 15%;
(6) distilled water 10%.
6. be used for resistance infectious disease medicine series
(1) paraplasm 60%, wherein Fructus Benincasae juice 30%, Thallus Laminariae (Thallus Eckloniae) juice 30%;
(2) enzyme promoter is Tween 80 10%;
(3) inorganic salt 5.5%, wherein NaNO
33%, KCl0.5%, FeSO
40.5%, KH
2PO
41.5%;
(4) compound enzyme 3%;
(5) adjuvant is a citric acid 5.5%;
(6) distilled water 16%.
7. be used for health-care series
(1) paraplasm 56.5%, wherein Ovum Gallus domesticus album 10%, Lac Bovis seu Bubali 10%, pineapple juice 30%, Oleum Glycines 1.5%, Semen Maydis pulp 5%;
(2) enzyme promoter is Tween 80 10%;
(3) inorganic salt KH
2PO
41%;
(4) compound enzyme 2%;
(5) adjuvant 18%, wherein peptone 5%, glycerol 2%, yeast extract 6%, glucose 5%;
(6) distilled water 12.5%.
8. be used for the removing dampness by means of aromatics medicine
(1) paraplasm 28%, wherein radish Juice 10%, Sucus Pyri 10%, beerwort 5%, Semen Maydis pulp 3%;
(2) enzyme promoter is Tween 80 10%;
(3) inorganic salt 2%, wherein (NH
4)
2SO
40.5%, MgSO
40.5%, MnSO
40.5%, CaCO
30.5%;
(4) compound enzyme 2%;
(5) adjuvant 30%, wherein Lac regis apis 1%, yeast extract 2%;
(6) distilled water 60%.
9. be used for the tonification Yang invigorating medicine
(1) paraplasm 20%, wherein Lac Bovis seu Bubali 5%, cabbage juice 10%, Semen Nelumbinis juice 5%;
(2) enzyme promoter is Tween 80 6%;
(3) inorganic salt 10%, wherein Na
2HPO
41%, NaCl1%, NaNO
34%, (NH
4)
2SO
44%;
(4) compound enzyme 5%;
(5) adjuvant 9%, wherein agar 2%, glucose 5%, yeast extract 2%;
(6) distilled water 50%.
10. be used for the single Chinese herbal medicine
(1) paraplasm 15%, wherein Sucus Mali pumilae 10%, bean sprout juice 5%;
(2) enzyme promoter is Tween 80 5%;
(3) inorganic salt 3%, wherein K
2HPO
41%, KH
2PO
41%, (NH
4)
2SO
40.5%, MnSO
40.5%;
(4) compound enzyme 5%;
(5) adjuvant 22%, wherein egg albumen powder 5%, yeast extract 5%, glucose 5%, white sugar 2%, lactic acid 5%;
(6) distilled water 50%.
The preparation method of compound enzyme reactant liquor of the present invention: produce compound enzyme earlier, then paraplasm, the inorganic salt that contains trace element, compound enzyme, adjuvant, distilled water are joined in the container in proportion and mix, cradle vibrate 8 hours, cultivated 8 hours for 45 ℃, bottling, ultraviolet radiation 8 hours can be preserved 18 months 18-26 ℃ of following lucifuge.
Compound enzyme reactant liquor of the present invention is nontoxic, biodegradability is good, can be widely used in medicine, health product, food, beverage, by to its raw material enzymolysis and fermentation, can be with active ingredient in the raw material, chemical analysis effectively extracts, as be used for Chinese herbal medicine, under general temperature and pressure, carry out enzyme digestion reaction with compound enzyme reactant liquor of the present invention, the active ingredient of being extracted than traditional decocting herb method improves greatly, thereby drug effect is increased greatly, and the most of material in the depleted medicinal residues of traditional method can be converted into complementary glucose of curing the disease and be absorbed by the body; Compound enzyme reactant liquor of the present invention because to the enzymatic hydrolysis and fermentation of raw material, the product molecular structure has played radical change, and bioactive substance quality such as small molecular protein free amino acid, linolenic acid polypeptide are improved greatly, is easilier absorbed by the people; Adopting compound enzyme reactant liquor of the present invention to carry out biotechnological pharmaceutics does not have waste water,waste gas and industrial residue, and environmental conservation is had significance.
The specific embodiment
Further specify characteristics of the present invention below by instantiation
Example 1
This example is to produce the compound enzyme reactant liquor that is used for health product series.
With 10Kg Ovum Gallus domesticus album, 10Kg Lac Bovis seu Bubali, 30kg pineapple juice, 1.5 kilograms of Oleum Glycines, 5kg Semen Maydis pulp, Tween 80 10Kg, KH
2PO
41Kg, compound enzyme (pectase: cellulase: glucose isomerase: amylase: lipase: weight ratio is 2: 2: 3: 3: 3) 2Kg, peptone 5Kg, glycerol 2kg, glucose 5Kg, yeast extract 6Kg, distilled water 12.5Kg, mixing in container, cultivated 8 hours for 45 ℃, bottling, ultraviolet radiation 8 hours can be preserved 8 months 18-28 ℃ of following lucifuge.
Example 2
This example is to produce the compound enzyme reactant liquor that is used for antiaging agent
Used raw material: Sucus Mali pumilae 1Kg, Tween 80 0.5Kg, Hongqu powder (red colouring agent) 0.5Kg, KH
2PO
40.01Kg, MgSO
40.025Kg, NH
4NO
30.25kg, CuSO
40.02Kg, FeSO
40.02Kg, ZnSO
40.02Kg, MnSO
40.02Kg, compound enzyme 0.2Kg, brown sugar 0.36Kg, agar 0.2Kg, citric acid 0.375Kg, distilled water 6.5Kg, preparation method is with example 1.
Example 3
This example is to produce the compound enzyme reactant liquor that is used for cancer therapy drug series.
Raw material: Radix Dauci Sativae juice 1Kg, Tween 80 0.5Kg, Hongqu powder (red colouring agent) 0.5Kg, MgSO
40.1Kg, KH
2PO
40.1Kg, (NH
4)
2HPO
40.2Kg, KCl0.1Kg, NH
4NO
30.2Kg, citric acid 2Kg, agar 0.2Kg, peptone 0.2Kg, compound enzyme 0.4Kg, distilled water 4.5Kg, preparation method is with example 1.
Example 4
This example is to produce the compound enzyme reactant liquor that is used for resistance infectious disease medicine.
Raw material: Fructus Benincasae juice 3Kg, Thallus Laminariae (Thallus Eckloniae) juice 3Kg, Tween 80 1Kg, NaNO
30.3Kg, KCl0.05Kg, FeSO
40.05Kg, KH
2PO
40.15Kg, compound enzyme 0.3Kg, citric acid 0.55Kg, distilled water 1.6Kg, preparation method is with example 1.
Example 5
This example is to produce the compound enzyme reactant liquor that the single Chinese herbal medicine uses.
Raw material: Sucus Mali pumilae 10Kg, bean sprout juice 5Kg, Tween 80 5Kg, K
2HPO
41Kg, KH
2PO
41Kg, (NH
4)
2SO
40.5Kg, MnSO
40.5Kg, compound enzyme 5Kg, egg albumen powder 5Kg, yeast extract 5Kg, glucose 5Kg, white sugar 2Kg, lactic acid 5Kg, distilled water 50Kg, preparation method is with example 1.
Example 6
This example is an application example.
With 1 liter of Radix Astragali (being crushed to 60 orders) of compound enzyme reactant liquor of example 5 with 20Kg stir evenly the back under 36 ± 5 ℃ with ferment 72 hours, and, extract supernatant after the sedimentation with NaOH or acetic acid control PH3.5～6.2.
The decocting herb method is extracted Radix Astragali decoction liquor routinely.
Analysis result shows, with the astragalus polysaccharides extraction ratio of compound enzyme reactant liquor enzymolysis of the present invention is that 5.046 ± 0.365 times of conventional decocting herb method (are to be tested by central laboratory of Colleges Of Traditional Chinese Medicine Of Fujian, the employing colorimetry detects, and used instrument is ultraviolet/visible spectrophotometer).
Example 7
This example is to use practicality.
With the method that Radix Glycyrrhizae is pressed example 6, promptly use the enzyme solution and the conventional decocting herb method of example 5 compound enzyme reactant liquors, compare the extraction ratio of glycyrrhizin in two kinds of method extracting solution.The result shows that the extraction ratio of the glycyrrhizin of enzyme solution is 2.20 ± 0.15 times (be by the test of central laboratory of Colleges Of Traditional Chinese Medicine Of Fujian, adopt high performance liquid chromatography to detect, used instrument is a U.S. tsp high performance liquid chromatograph) of conventional normal decocting method.
Claims (13)
1. compound enzyme reactant liquor is characterized in that constituent and content thereof (is 100 in the composition weight sum):
Compound enzyme 2-5%, paraplasm 4.5-60%, enzyme promoter 5-10%, distilled water 10-70%, the inorganic salt 0.5-16% that contains trace element, adjuvant 3-24%,
Described compound enzyme be cellulase, pectase, glucose isomerase, amylase, lipase with 2: 2: 3: the mixed enzyme of 3: 3 weight ratios,
Described paraplasm comprises a kind of, two or more the mixture of Semen Maydis pulp, Semen Maydis powder, Radix Dauci Sativae juice, Ovum Gallus domesticus album, Sucus Mali pumilae, Semen sojae atricolor powder, Sucus Pyri, radish Juice, green vegetable juice, Succus Momordicae charantiae, Oleum Glycines, Lac Bovis seu Bubali, beerwort, cabbage juice, Semen Nelumbinis juice, bean sprout juice
Described enzyme promoter comprises a kind of, two or more the mixture of yeast extract, Tween 80, Hongqu powder (red colouring agent),
Described inorganic salt comprises KH
2PO
4, (NH
4)
2HPO
4, KNO
3, MgSO
4, Na
2SO
4, ZnSO
4, CaCL
2, (NH
4)
2SO
4, (NH
4)
2NO
3, KCl, FeSO
4At interior a kind of, two or more mixture,
Described adjuvant comprises a kind of, two or more the mixture of peptone, egg albumen powder, glycerol, yeast extract, glucose, Lac regis apis, agar, lactic acid, white sugar, brown sugar.
2. according to the described compound enzyme reactant liquor of claim 1, it is characterized in that constituent and content thereof (is 100 in the composition weight sum):
(1) paraplasm 4.8%, wherein Semen Maydis pulp 1.8%, Radix Dauci Sativae juice 1%, Ovum Gallus domesticus album 2%;
(2) enzyme promoter 6.5%, wherein yeast extract 3%, Tween 80 1.5%, Hongqu powder (red colouring agent) 2%;
(3) inorganic salt 7.4%, wherein KH
2PO
40.1%, KH
2PO
40.1%, MgSO
41.5%, Ca
2SO
44.5%, (NH
4)
2SO
40.7%, KCl 0.5%;
(4) compound enzyme 2%;
(5) adjuvant 9.3%, wherein peptone 2%, glucose 6%, Lac regis apis 1.3%;
(6) distilled water 70%.
3. according to the described compound enzyme reactant liquor of claim 1, it is characterized in that constituent and content thereof (is 100 in the composition weight sum):
(1) paraplasm is Sucus Mali pumilae 10%;
(2) enzyme promoter 10%, wherein Tween 80 5%, Hongqu powder (red colouring agent) 5%;
(3) inorganic salt 3.65%, wherein KH
2PO
40.1%, MgSO
40.25%, NH
4NO
32.5%, CuSO
40.2%, ZnSO
40.2%, FeSO
40.2%, MnSO
40.2%;
(4) compound enzyme 2%;
(5) adjuvant 9.35%, wherein brown sugar 3.6%, agar 2%, citric acid 3.75%;
(6) distilled water 65%.
4. according to the described compound enzyme reactant liquor of claim 1, it is characterized in that constituent and content thereof (is 100 in the composition weight sum):
(1) paraplasm 32.6%, wherein Semen sojae atricolor powder 5%, Semen Maydis powder 5%, Sucus Pyri 10%, Sucus Mali pumilae 10%, Ovum Gallus domesticus album 2.6%;
(2) enzyme promoter is Tween 80 5%;
(3) inorganic salt 0.6%, wherein KH
2PO
40.2%, NaCl 0.2%, (NH
4)
2SO
40.2%;
(4) compound enzyme 2%;
(5) adjuvant 10.8%, wherein agar 2%, citric acid 3.8%, peptone 5%;
(6) distilled water 49%;
5. according to the described compound enzyme reactant liquor of claim 1, it is characterized in that constituent and content thereof (is 100 in the composition weight sum):
(1) paraplasm is Radix Dauci Sativae juice 10%;
(2) enzyme promoter 10%, wherein Tween 80 5%, Hongqu powder (red colouring agent) 5%;
(3) compound enzyme 4%;
(4) adjuvant 24%, wherein citric acid 20%, agar 2%, peptone 2%;
(5) inorganic salt 7%, wherein MgSO
41%, KH
2PO
41%, (NH
4)
2HPO
42%, KCl1%, NH
4NO
a2%;
(6) distilled water 45%;
6. according to the described compound enzyme reactant liquor of claim 1, it is characterized in that constituent and content thereof (is 100 in the composition weight sum):
(1) paraplasm 50%, wherein Sucus Pyri 20%, radish Juice 10%, green vegetable juice 10%, Succus Momordicae charantiae 10%;
(2) enzyme promoter is Tween 80 5%;
(3) inorganic salt 16%, wherein ZnSO
48%, MgSO
42%, KH
2PO
46%;
(4) compound enzyme 4%;
(5) adjuvant is a lactic acid 15%;
(6) distilled water 10%.
7. according to the described compound enzyme reactant liquor of claim 1, it is characterized in that constituent and content thereof (is 100 in the composition weight sum):
(1) paraplasm 60%, wherein Fructus Benincasae juice 30%, Thallus Laminariae (Thallus Eckloniae) juice 30%;
(2) enzyme promoter is Tween 80 10%;
(3) inorganic salt 5.5%, wherein NaNO
33%, KCl0.5%, FeSO
40.5%, KH
2PO
41.5%;
(4) compound enzyme 3%;
(5) adjuvant is a citric acid 5.5%;
(6) distilled water 16%.
8. according to the described compound enzyme reactant liquor of claim 1, it is characterized in that constituent and content thereof (is 100 in the composition weight sum);
(1) paraplasm 56.5%, wherein Ovum Gallus domesticus album 10%, Lac Bovis seu Bubali 10%, pineapple juice 30%, Oleum Glycines 1.5%, Semen Maydis pulp 5%;
(2) enzyme promoter is Tween 80 10%;
(3) inorganic salt KH
2PO
41%;
(4) compound enzyme 2%;
(5) adjuvant 18%, wherein peptone 5%, glycerol 2%, yeast extract 6%, glucose 5%;
(6) distilled water 12.5%.
9. according to the described compound enzyme reactant liquor of claim 1, it is characterized in that constituent and content thereof (is 100 in the composition weight sum):
(1) paraplasm 28%, wherein radish Juice 10%, Sucus Pyri 10%, beerwort 5%, Semen Maydis pulp 3%;
(2) enzyme promoter is Tween 80 5%;
(3) inorganic salt 2%, wherein (NH
4)
2SO
40.5%, MgSO
40.5%, MnSO
40.5%, CaCO
30.5%;
(4) compound enzyme 2%;
(5) adjuvant 3%, wherein Lac regis apis 1%, yeast extract 2%;
(6) distilled water 60%.
10. according to the described compound enzyme reactant liquor of claim 1, it is characterized in that constituent and content thereof (is 100 in the composition weight sum):
(1) paraplasm 20%, wherein Lac Bovis seu Bubali 5%, cabbage juice 10%, Semen Nelumbinis juice 5%;
(2) enzyme promoter is Tween 80 6%;
(3) inorganic salt 10%, wherein Na
2HPO
41%, NaCl1%, NaNO
34%, (NH
4)
2SO
44%;
(4) compound enzyme 5%;
(5) adjuvant 9%, wherein agar 2%, glucose 5%, yeast extract 2%;
(6) distilled water 50%.
11., it is characterized in that constituent and content thereof (is 100 in the composition weight sum) according to the described compound enzyme reactant liquor of claim 1:
(1) paraplasm 15%, wherein Sucus Mali pumilae 10%, bean sprout juice 5%;
(2) enzyme promoter is Tween 80 5%;
(3) inorganic salt 3%, wherein K
2HPO
41%, KH
2PO
41%, (NH
4)
2SO
40.5%, MnSO
40.5%;
(4) compound enzyme 5%;
(5) adjuvant 22%, wherein egg albumen powder 5%, yeast extract 5%, glucose 5%, white sugar 2%, lactic acid 5%;
(6) distilled water 50%.
12. the described compound enzyme reactant liquor of claim 1 can be used for Chinese herbal medicine, health product, food, beverage, by the fermented extracted effective ingredient wherein to its raw material.
13. the preparation method of the described compound enzyme reactant liquor of claim 1-11 comprises following process:
(1) produces compound enzyme: cellulase, pectase, glucose isomerase, amylase, lipase are mixed in proportion, its mix homogeneously are promptly obtained compound enzyme with shaking table;
(2) produce the compound enzyme reactant liquor: with step (1) produce compound enzyme and paraplasm, the inorganic salt, adjuvant, the distilled water that contain trace element be added in the container in proportion and mix, cradle vibrate 8 hours, cultivated 8 hours down for 40 ℃, bottling, ultraviolet radiation 8 hours can be preserved 18 months 18-26 ℃ of following lucifuge.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN01130990A CN1406630A (en) | 2001-08-29 | 2001-08-29 | Composite enzyme reacting liquid |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN01130990A CN1406630A (en) | 2001-08-29 | 2001-08-29 | Composite enzyme reacting liquid |
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| Publication Number | Publication Date |
|---|---|
| CN1406630A true CN1406630A (en) | 2003-04-02 |
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ID=4670299
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| CN01130990A Pending CN1406630A (en) | 2001-08-29 | 2001-08-29 | Composite enzyme reacting liquid |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100415982C (en) * | 2006-12-20 | 2008-09-03 | 江苏华信亚麻纺织有限公司 | Composite biological emzyme/amboceptor system and use for textile scouring process |
| CN100451126C (en) * | 2005-10-27 | 2009-01-14 | 三达膜科技（厦门）有限公司 | Process for separating protein, apple polyphenol, apple starch and pigment from apple juice |
| CN1806043B (en) * | 2004-04-08 | 2011-04-20 | 日清奥利友集团株式会社 | Lipase powder, methods for producing the same and use thereof |
| CN103952387A (en) * | 2014-03-31 | 2014-07-30 | 宁夏乙征生物工程有限公司 | Enzyme preparation composition for improving extraction rate of traditional Chinese medicine |
| CN104207149A (en) * | 2014-08-06 | 2014-12-17 | 金寨县万紫千红农业科技开发有限公司 | Food-use organic enzyme and preparation method thereof |
| CN104784233A (en) * | 2015-04-16 | 2015-07-22 | 珲春绿源参业生物科技有限公司 | Method for preparing panaxoside and panaxynol through enzymatic zymotechnics |
| CN106148057A (en) * | 2015-04-10 | 2016-11-23 | 天津乾鼎华科技发展有限责任公司 | A kind of dark beer and brewing method thereof |
| CN110583696A (en) * | 2019-09-11 | 2019-12-20 | 山东捷利尔肥业有限公司 | Biological enzyme for improving appearance and color of pomegranate |
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2001
- 2001-08-29 CN CN01130990A patent/CN1406630A/en active Pending
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1806043B (en) * | 2004-04-08 | 2011-04-20 | 日清奥利友集团株式会社 | Lipase powder, methods for producing the same and use thereof |
| CN100451126C (en) * | 2005-10-27 | 2009-01-14 | 三达膜科技（厦门）有限公司 | Process for separating protein, apple polyphenol, apple starch and pigment from apple juice |
| CN100415982C (en) * | 2006-12-20 | 2008-09-03 | 江苏华信亚麻纺织有限公司 | Composite biological emzyme/amboceptor system and use for textile scouring process |
| CN103952387A (en) * | 2014-03-31 | 2014-07-30 | 宁夏乙征生物工程有限公司 | Enzyme preparation composition for improving extraction rate of traditional Chinese medicine |
| CN103952387B (en) * | 2014-03-31 | 2017-03-15 | 宁夏乙征生物工程有限公司 | A kind of combination of enzyme preparations thing for improving Chinese medicine extraction rate |
| CN104207149A (en) * | 2014-08-06 | 2014-12-17 | 金寨县万紫千红农业科技开发有限公司 | Food-use organic enzyme and preparation method thereof |
| CN104207149B (en) * | 2014-08-06 | 2016-06-01 | 金寨县万紫千红农业科技开发有限公司 | Food organic enzyme and its preparation method |
| CN106148057A (en) * | 2015-04-10 | 2016-11-23 | 天津乾鼎华科技发展有限责任公司 | A kind of dark beer and brewing method thereof |
| CN104784233A (en) * | 2015-04-16 | 2015-07-22 | 珲春绿源参业生物科技有限公司 | Method for preparing panaxoside and panaxynol through enzymatic zymotechnics |
| CN110583696A (en) * | 2019-09-11 | 2019-12-20 | 山东捷利尔肥业有限公司 | Biological enzyme for improving appearance and color of pomegranate |
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