CN1387876A - Clover extract, composition with the extract and their application in animal health - Google Patents
Clover extract, composition with the extract and their application in animal health Download PDFInfo
- Publication number
- CN1387876A CN1387876A CN01118421A CN01118421A CN1387876A CN 1387876 A CN1387876 A CN 1387876A CN 01118421 A CN01118421 A CN 01118421A CN 01118421 A CN01118421 A CN 01118421A CN 1387876 A CN1387876 A CN 1387876A
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- CN
- China
- Prior art keywords
- extract
- herba medicaginis
- application
- animal
- herba
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 241001465754 Metazoa Species 0.000 title claims abstract description 22
- 239000000203 mixture Substances 0.000 title claims abstract description 11
- 241000219793 Trifolium Species 0.000 title abstract 5
- 230000036737 immune function Effects 0.000 claims abstract description 5
- 238000000034 method Methods 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 8
- 238000001694 spray drying Methods 0.000 claims description 4
- 238000001291 vacuum drying Methods 0.000 claims description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 238000002360 preparation method Methods 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims 1
- 238000000605 extraction Methods 0.000 abstract description 3
- 230000001105 regulatory effect Effects 0.000 abstract description 3
- 238000005728 strengthening Methods 0.000 abstract description 3
- 230000001737 promoting effect Effects 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 7
- 239000000047 product Substances 0.000 description 7
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 241000196324 Embryophyta Species 0.000 description 3
- 210000004698 lymphocyte Anatomy 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 238000004537 pulping Methods 0.000 description 3
- 241000699670 Mus sp. Species 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000007365 immunoregulation Effects 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 238000012856 packing Methods 0.000 description 2
- 230000035755 proliferation Effects 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 235000013343 vitamin Nutrition 0.000 description 2
- 239000011782 vitamin Substances 0.000 description 2
- 229940088594 vitamin Drugs 0.000 description 2
- 229930003231 vitamin Natural products 0.000 description 2
- 150000003722 vitamin derivatives Chemical class 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- 208000031295 Animal disease Diseases 0.000 description 1
- 244000025254 Cannabis sativa Species 0.000 description 1
- 206010015719 Exsanguination Diseases 0.000 description 1
- 239000012980 RPMI-1640 medium Substances 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 238000010171 animal model Methods 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 229930003944 flavone Natural products 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 235000011949 flavones Nutrition 0.000 description 1
- 239000004459 forage Substances 0.000 description 1
- 230000002519 immonomodulatory effect Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 239000003226 mitogen Substances 0.000 description 1
- 235000021049 nutrient content Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 239000001397 quillaja saponaria molina bark Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 229930182490 saponin Natural products 0.000 description 1
- 150000007949 saponins Chemical class 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 210000004988 splenocyte Anatomy 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The present invention relates to one kind of care product for animals health and includes clover extract as animal health-care product, extraction process of the clover extract, composition containing the clover extract and the application of the clover extract in preparing animal health product, especially in preparing product with the functions of regulating animal's immunological function, strengthening their resistance and promoting their growth.
Description
Herba Medicaginis is a kind of perennial leguminous plant, contains rich in protein, vitamin and trace element in the stem and leaf, and nutritive value is very high, generally uses as high quality forage.In the cultivated area of China about 2,000 ten thousand mu, per mu yield Radix Glycyrrhizae 600-800 kilogram, about 1,400 ten thousand tons of annual output.Past is to the mode of utilizing of Herba Medicaginis, adopts generally that green grass or young crops is raised, ensiling or hay curing, and range of application is narrower, and added value is not high.
There is significant difference in nutrient composition content in Herba Medicaginis stem, the leaf, and the nutrient content in the leaf is generally 1-1.5 times in the stem.A large amount of leaves takes place in regular meeting in the process of dry in the sun and collection, thereby causes a large amount of losses of Herba Medicaginis nutritional labeling.In addition, in the past in the actual production, because processing and storage requirement are simple and crude, the mildew and rot phenomenon rotten and that weeds, earth mix up wherein of Herba Medicaginis happens occasionally, thereby its using value seriously reduces, even thoroughly loses its using value in feedstuff, not only brings about great losses economically, and causing the significant wastage of resource, this unreasonable resource that causes and economic loss owing to the post-production technology is painful especially.Therefore, study a kind of practicablely, can greatly keep the process technology of Herba Medicaginis nutritive value to have very important practical significance.
The food-safety problem that causes owing to abuse feed additive in feedstuff takes place repeatedly in recent years, not only causes the tremendous economic loss, and people are had a lingering fear when the consumption livestock products.In order to research and develop feed technology new, safety, we improve the natural plants and the composition of animal disease resistant ability and study with health role.Find that under study for action Herba Medicaginis extract has significant regulating action to the immune function of animal, and researched and developed successfully a kind of Herba Medicaginis extract product and extraction process technology thereof that is used for animal health.This technology, Products Development success not only for Herba Medicaginis in herding is produced, opened up more extensive, more the application prospect of economic worth arranged, and opened up new way for the medical value of exploitation Herba Medicaginis resource.
The present invention relates to a kind of Herba Medicaginis extract and extraction process technology thereof that is used for animal health.Extracting method of the present invention comprises, Herba Medicaginis pulverized or add an amount of water (ratio of Herba Medicaginis and water is preferably 1: the 0.1-20 weight portion), make pulpous state, the liquid part is collected in centrifugal back, and dry (preferably spray drying or vacuum drying) promptly gets required Herba Medicaginis extract then.
By the Herba Medicaginis extract that the inventive method obtains, its main component comprises protein (40-50%), vitamin summation (0.3-2%), the total Saponin of Herba Medicaginis (0.3-4%), total flavones (1-4%).
The invention still further relates to the compositions that contains described Herba Medicaginis extract, said composition contains the Herba Medicaginis extract of 0.01-10%, preferably contains the Herba Medicaginis extract of 1-10%.
The invention still further relates to Herba Medicaginis extract is preparing the animal health product, is especially regulating the animal immune function to strengthen the application in its resistance and the somatotrophic product in preparation.
Method of the present invention and the Herba Medicaginis extract that makes with this method have the following advantages: the one, changed the Herba Medicaginis process technology.Utilize process technology of the present invention, can greatly keep and obtain the multiple composition in the Herba Medicaginis, the wasting of resources and the economic loss that have caused owing to fallen leaves and going rotten etc. before having avoided.The 2nd, found that Herba Medicaginis extract has immunoloregulation function.The Herba Medicaginis extract that adopts above-mentioned process technology to produce has significant immunoregulation effect, can be used for strengthening the resistance of animal.Technical products of the present invention has following characteristics
One, Herba Medicaginis process technology of the present invention have simple and easy to do, do not have harmful chemical agent, natural component destroyed advantages such as little, that loss is few.
Two, Herba Medicaginis extract of the present invention has significant immunoregulation effect to animal body, when in feedstuff, adding by a certain percentage, animal had the effect of the resistance of enhancing, the long-term interpolation do not produce any toxic side effects to animal, is a kind of pure natural, non-harmful animal health product.
Three, Herba Medicaginis extract of the present invention has the proteinic effect of supplement feed when strengthening the animal immune function.
Embodiment 1
Take by weighing 10 kilograms of fresh Herba Medicaginiss, directly pulverize pulping, or add 10 kilograms of entry, pulverize until pulping, filter or centrifugal after, liquid hold-up carries out vacuum drying or spray drying with liquid, pulverizes the back packing.
Embodiment 2
Take by weighing 10 kilograms of dried Herba Medicaginiss.Add 200 kilograms of entry, soaked 5-30 minute, pulverize until pulping, filter or centrifugal after, liquid hold-up carries out vacuum drying or spray drying with liquid, pulverizes the back packing.Herba Medicaginis extract is to animal lymph cytoactive impact effect
Get the some grams of Herba Medicaginis extract among the embodiment 1, BALB/C (H-2
d) mouse inbred lines is laboratory animal, carries out immunological testing.
Experimental technique: with the experiment mice sacrificed by exsanguination, aseptic cuing open got spleen, puts in Hank ' the s liquid, makes single cell suspension, uses Hank ' s liquid washing 2 times, is transferred to desired concn with RPMI-1640.The mouse lymphocyte for preparing is added in the 96 porocyte culture plates, and 100 μ l/ holes add Herba Medicaginis extract then, 100 μ l/ holes, and each handles 3 multiple holes, 37 ℃, 5%CO
2Cultivate 72hr under the condition, the MTT method is measured.
Experimental result: Herba Medicaginis extract has the splenocyte of white mice and to promote proliferation function (table 1) extremely significantly when 25 μ g/ml and 100 μ g/ml.
Table 1 Herba Medicaginis extract is to the active result that influences of mouse lymphocyte
| Handle | Dosage (μ g/ml) | OD value: XSD |
| ?CK | ?0 | ?0.276±0.001 |
| Herba Medicaginis extract | ?25 | ?0.332±0.02 ** |
| ?100 | ?0.325±0.02 ** |
**p＜0.01
Conclusion: Herba Medicaginis extract has mouse lymphocyte and promotes proliferation function extremely significantly, shows the effect of mitogen sample, and animal is had immunological enhancement.
Claims (7)
1. Herba Medicaginis extract is characterized in that this extract can obtain through following method: Herba Medicaginis is pulverized or add an amount of water, make pulpous state, the liquid part is collected in centrifugal back, and is dry then.
2. the Herba Medicaginis extract of claim 1, it is characterized in that: the ratio of Herba Medicaginis and water is 1: the 0.1-20 weight portion.
3. the Herba Medicaginis extract of claim 1 is characterized in that described drying is spray drying or vacuum drying.
4. the compositions that contains the Herba Medicaginis extract of claim 1 wherein contains the Herba Medicaginis extract of 0.01-10%.
5. the compositions of claim 4 wherein contains the Herba Medicaginis extract of 1-10%.
6. the application of the Herba Medicaginis extract of claim 1 in preparation animal health product.
7. the application of claim 6, described application are to regulate the animal immune function to strengthen its resistance and to promote its growth.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011184213A CN1204902C (en) | 2001-05-30 | 2001-05-30 | Clover extract, composition with the extract and their application in animal health |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011184213A CN1204902C (en) | 2001-05-30 | 2001-05-30 | Clover extract, composition with the extract and their application in animal health |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1387876A true CN1387876A (en) | 2003-01-01 |
| CN1204902C CN1204902C (en) | 2005-06-08 |
Family
ID=4663165
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNB011184213A Expired - Fee Related CN1204902C (en) | 2001-05-30 | 2001-05-30 | Clover extract, composition with the extract and their application in animal health |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1204902C (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222548A (en) * | 2013-05-22 | 2013-07-31 | 湖南省五季风生物科技有限责任公司 | Composite plant extractive feed additive and application thereof |
| CN103689608A (en) * | 2013-12-31 | 2014-04-02 | 大连民族学院 | Nutritional composition containing alfalfa leaf protein and preparation method of nutritional composition |
| CN104431373A (en) * | 2014-12-26 | 2015-03-25 | 北京爱绿生物科技有限公司 | Feed additive for improving quality of animal milk |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103168746B (en) * | 2012-11-28 | 2014-09-03 | 颜尚雄 | Method for producing taxol cow products and sheep products |
-
2001
- 2001-05-30 CN CNB011184213A patent/CN1204902C/en not_active Expired - Fee Related
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103222548A (en) * | 2013-05-22 | 2013-07-31 | 湖南省五季风生物科技有限责任公司 | Composite plant extractive feed additive and application thereof |
| CN103689608A (en) * | 2013-12-31 | 2014-04-02 | 大连民族学院 | Nutritional composition containing alfalfa leaf protein and preparation method of nutritional composition |
| CN104431373A (en) * | 2014-12-26 | 2015-03-25 | 北京爱绿生物科技有限公司 | Feed additive for improving quality of animal milk |
Also Published As
| Publication number | Publication date |
|---|---|
| CN1204902C (en) | 2005-06-08 |
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| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C56 | Change in the name or address of the patentee |
Owner name: INSTITUTE OF ANIMAL SCIENCES, CHINESE ACADEMY OF A Free format text: FORMER NAME: INST. OF ANIMAL HUSBANDRY, CHINESE ACADEMY OF AGRICULTURAL SCIENCES |
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| CP01 | Change in the name or title of a patent holder |
Address after: 100094 Beijing Old Summer Palace West Road, Haidian District, No. 2 Patentee after: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences Address before: 100094 Beijing Old Summer Palace West Road, Haidian District, No. 2 Patentee before: Inst. of Animal Husbandry, Chinese Academy of Agricultural Sciences |
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| ASS | Succession or assignment of patent right |
Owner name: DEYANG SANFENGYUAN MEAT PRODUCT SPECIALIZED COOPER Free format text: FORMER OWNER: INSTITUTE OF ANIMAL SCIENCES, CHINESE ACADEMY OF AGRICULTURAL SCIENCES Effective date: 20121108 |
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| C41 | Transfer of patent application or patent right or utility model | ||
| COR | Change of bibliographic data |
Free format text: CORRECT: ADDRESS; FROM: 100094 HAIDIAN, BEIJING TO: 618000 DEYANG, SICHUAN PROVINCE |
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| TR01 | Transfer of patent right |
Effective date of registration: 20121108 Address after: 618000 No. 804, Huashan building, North Huashan Road, Sichuan, Deyang Patentee after: Deyang Mitutoyo raw meat professional cooperatives Address before: 100094 Beijing Old Summer Palace West Road, Haidian District, No. 2 Patentee before: Institute of Animal Sciences, Chinese Academy of Agricultural Sciences |
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| EE01 | Entry into force of recordation of patent licensing contract |
Application publication date: 20030101 Assignee: Sichuan Longhaiyang Animal Pharmaceutical Co., Ltd. Assignor: Deyang Mitutoyo raw meat professional cooperatives Contract record no.: 2013510000090 Denomination of invention: Clover extract, composition with the extract and their application in animal health Granted publication date: 20050608 License type: Exclusive License Record date: 20130912 |
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| LICC | Enforcement, change and cancellation of record of contracts on the licence for exploitation of a patent or utility model | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20170527 Address after: 618000, No. 8, building 5, Huashan building, 17 North Huashan Road, Deyang, Sichuan Patentee after: Sichuan huatianbao Animal Biotechnology Co. Ltd. Address before: 618000 No. 804, Huashan building, North Huashan Road, Sichuan, Deyang Patentee before: Deyang Mitutoyo raw meat professional cooperatives |
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| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20050608 Termination date: 20190530 |
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| CF01 | Termination of patent right due to non-payment of annual fee |