CN1370235A - 编码参与碳代谢和能量产生的蛋白质的谷氨酸棒杆菌基因 - Google Patents
编码参与碳代谢和能量产生的蛋白质的谷氨酸棒杆菌基因 Download PDFInfo
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Abstract
本发明介绍了编码谷氨酸棒杆菌新型SMP蛋白的分离核酸分子(称为SMP核酸分子)。本发明还提供反义核酸分子、含有SMP核酸分子的重组表达载体以及导入了所述表达载体的宿主细胞。本发明还提供分离的SMP蛋白、突变SMP蛋白、融合蛋白、抗原性肽以及改进用谷氨酸棒杆菌生产需要化合物的方法,该方法的基础是遗传工程改进的谷氨酸棒杆菌SMP基因。
Description
相关申请
本申请要求1999年6月25日申请的美国临时专利申请顺序号60/141031、1999年7月9日申请的美国专利临时申请序号60/143208和1999年8月31日申请的美国专利临时申请序号60/151572的优先权。该申请还要求以下专利申请的优先权:1999年7月8日申请的德国专利申请号19931412.8、1999年7月8日申请的德国专利申请号19931413.6、1999年7月8日申请的德国专利申请号19931419.5、1999年7月8日申请的德国专利申请号19931420.9、1999年7月8日申请的德国专利申请号19931424.1、1999年7月8日申请的德国专利申请号19931428.4、1997年7月8日申请的德国专利申请号19931431.4、1999年7月8日申请的德国专利申请号19931433.0、1999年7月8日申请的德国专利申请号19931434.9、1999年7月8日申请的德国专利申请号19931510.8、1999年7月8日申请的德国专利申请号19931562.0、1999年7月8日申请的德国专利申请号19931634.1、1999年7月9日申请的德国专利申请号19932180.9、1999年7月9日申请的德国专利申请号19932227.9、1999年7月9日申请的德国专利申请号19932230.9、1999年7月14日申请的德国专利申请号19932924.9、1999年7月14日申请的德国专利申请号19932973.7、1999年7月14日申请的德国专利申请号19933005.0、1999年8月27日申请的德国专利申请号19940765.7、1999年9月3日申请的德国专利申请号19942076.9、1999年9月3日申请的德国专利申请号19942079.3、1999年9月3日申请的德国专利申请号19942086.6、1999年9月3日申请的德国专利申请号19942087.4、1999年9月3日申请的德国专利申请号19942088.2、1999年9月3日申请的德国专利申请号19942095.5、1999年9月3日申请的德国专利申请号19942123.4和1999年9月3日申请的德国专利申请号19942125.0。所有以上引用申请的全部内容均特别通过引用结合到本文中。
发明背景
在细胞中天然发生的代谢过程的某些产物和副产物在多种工业中具有实用性,所述工业包括食品工业、饲料工业、化妆品工业和制药业。这些分子统称为“精细化学品”,包括有机酸、蛋白质生成的和非蛋白质生成的氨基酸、核苷酸和核苷、脂质和脂肪酸、二元醇、糖类、芳族化合物、维生素和辅因子和酶。它们的生产最为方便的是通过开发用以生产和分泌大量的一种或多种需要分子的细菌的大规模培养来进行。用于此目的的一种特别有用的生物是谷氨酸棒杆菌(Corynebacterium glutamicum),这是一种革兰氏阳性的非致病菌。通过菌株的选择,已经开发了许多产生一系列需要化合物的突变型菌株。然而,选择在特定分子生产方面改进的菌株是一个费时且困难的过程。
发明概要
本发明提供具有多种用途的新型细菌核酸分子。这些用途包括鉴定可以用来生产精细化学品的微生物、调节谷氨酸棒杆菌或相关细菌产生精细化学品、定型或鉴定谷氨酸棒杆菌或相关细菌、用作谷氨酸棒杆菌基因组作图的参比点以及用作转化的标记。这些新型核酸分子编码本文中称为糖代谢和氧化磷酸化(sugar metabolism and_oxidativephosphorylation)(SMP)蛋白的蛋白质。
谷氨酸棒杆菌是一种革兰氏阳性需氧菌,通常用于大规模工业生产种类繁多的精细化学品,也用于降解碳氢化合物(例如用于处理石油溢出物)以及用于萜类化合物的氧化。因此,本发明的SMP核酸分子可以用来鉴定可以例如通过发酵过程生产精细化学品的微生物。本发明SMP核酸表达的调节或本发明SMP核酸分子序列的修饰,可以用以调节微生物的一种或多种精细化学品的生产(例如提高各种棒杆菌或短杆菌的一种或多种精细化学品的产率或产量)。
本发明的SMP核酸也可以用来鉴定谷氨酸棒杆菌或其密切相关菌种的生物,或用来鉴定混合微生物群体中谷氨酸棒杆菌或其相关菌种存在与否。本发明提供多种谷氨酸棒杆菌基因的核酸序列;通过用跨越谷氨酸棒杆菌特有基因的区段的探针,在严格条件下探测特有微生物群体培养物或混合微生物群体培养物的提取的基因组DNA,人们可以确定该生物是否存在。虽然谷氨酸棒杆菌本身是非致病性的,但它与人类中的致病菌种例如白喉棒杆菌(Corynebacterium diphtheriae)(白喉病原体)有关;对这类生物的检测具有重要临床意义。
本发明的SMP核酸分子也可以用作谷氨酸棒杆菌基因组或相关生物基因组作图的参比点。同样,这些分子及其变异体或其部分可以用作遗传工程棒杆菌或短杆菌菌种的标记。
本发明新型核酸分子编码的SMP蛋白能够在谷氨酸棒杆菌中例如执行涉及碳化合物(例如糖)代谢的功能,或执行涉及通过诸如氧化磷酸化过程产生能量分子的功能。已知可得到用于谷氨酸棒杆菌的克隆载体例如Sinskey等的美国专利号4,649,119公开的克隆载体以及用于谷氨酸棒杆菌和相关短杆菌菌种(例如乳发酵短杆菌(Brevibacteriumlactofermentum))的遗传操作技术(Yoshihama等,J.Bacteriol.162:591-597(1985);Katsumata等,J.Bacteriol.159:306-311(1984);和Santamaria等,J.Gen.Microbiol.130:2237-2246(1984)),则本发明的核酸分子可以用于这种生物的基因工程,以使其成为一种或多种精细化学品的更好或更有效的生产菌。这种精细化学品产量或生产效率提高可能是由于本发明基因操作的直接效应引起的,或者可能是由这类操作的间接效应引起的。
本发明SMP蛋白改变可能通过多种机制直接影响掺入这种改变蛋白的谷氨酸棒杆菌菌株产生精细化学品的产率、产量和/或生产效率。诸如糖的高能碳分子降解以及诸如NADH和FADH2的化合物通过氧化磷酸化转化为含有高能磷酸键的化合物可以产生多种化合物,这些化合物本身可能就是需要的精细化学品,如丙酮酸、ATP、NADH和许多中间体糖化合物。细胞可利用这些代谢途径产生的能量分子(如ATP)和还原当量物(如NADH或NADPH)驱动在能量上不利的反应。这样的不利反应包括许多精细化学品生物合成途径。人们通过改进细胞利用特定糖的能力(例如对编码参与细胞降解该糖并将其转化为能量的酶的基因进行操作)可提高能量,以便细胞可进行不利但却需要的代谢反应(例如生物合成需要精细化学品)
诱变一种或多种本发明SMP基因也可能产生活性改变的SMP蛋白,活性改变的SMP蛋白间接影响谷氨酸棒杆菌产生一种或多种需要精细化学品。例如,人们通过提高谷氨酸棒杆菌利用一种或多种糖的效率(从而增强所述糖转化为有用的能量分子)、或者提高还原当量物转化为有用的能量分子的效率(例如通过提高氧化磷酸化效率或增强ATP合酶活性),能够增加这些高能化合物量,供细胞驱动通常不利的代谢过程。这样的过程包括构建细胞壁、转录、翻译以及生物合成细胞生长分裂必需的化合物(例如核苷酸、氨基酸、维生素、脂质等)(Lengeler等(1999)Biology of Prokaryotes,Thieme Verlag:Stuttgart,第88-109、913-918、875-899页)。通过改进这些工程细胞的生长和繁殖,既可提高大规模培养物中的细胞存活力,也可以提高其分裂率,这样在发酵罐培养物中能够存活更大量的细胞。至少因为存在更多的产生需要精细化学品的活细胞而提高产率、产量或生产效率。此外,细胞利用糖发酵过程产生的许多降解产物作为产生其它需要产物如精细化学品的前体或中间体。因而,由于增强了细胞代谢糖的能力,从而也可以增加供细胞其它过程用的降解产物数量。
本发明提供编码本文称为SMP蛋白的新型核酸分子,所述SMP蛋白能够例如执行参与谷氨酸棒杆菌诸如糖的碳水化合物代谢和通过诸如氧化磷酸化过程产生能量分子的功能。编码SMP蛋白的核酸分子在本文中称为SMP核酸分子。在一个优选实施方案中,所述SMP蛋白参与碳分子及其降解产物转化为细胞用于代谢过程的能量。这类蛋白的实例包括由表1给出基因编码的蛋白。
因此,本发明的一个方面涉及分离的核酸分子(例如cDNA、DNA或RNA),所述分离的核酸分子包含编码SMP蛋白或其生物活性部分的核苷酸序列,还涉及适合作为检测或扩增SMP编码核酸(例如DNA或mRNA)的引物或杂交探针的核酸片段。在特别优选的实施方案中,所述分离的核酸分子包含序列表奇数SEQ ID NO(如SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:7…)中所述核苷酸序列之一、或这些核苷酸序列之一的编码区或其互补序列。在其它特别优选的实施方案中,本发明的分离的核酸分子包含这样的核苷酸序列或其部分:所述核苷酸序列与一种序列表奇数SEQ ID NO(如SEQ IDNO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:7…)核苷酸序列杂交或与其具有至少约50%、优选至少约60%、更优选至少约70%、80%或90%、甚至更优选至少约95%、96%、97%、98%、99%或更高同源性。在其它优选实施方案中,所述分离的核酸分子编码一种序列表偶数SEQ ID NO(如SEQ ID NO:2、SEQ ID NO:4、SEQ ID NO:6、SEQ IDNO:8…)氨基酸序列。本发明优选的SMP蛋白也最好具有至少一种本文所述的SMP活性。
在另一实施方案中,所述分离的核酸分子编码一种蛋白质或其部分,其中所述蛋白或其部分包括与本发明氨基酸序列(如具有序列表偶数SEQ ID NO的序列)足够同源的氨基酸序列,例如与本发明的氨基酸序列足够同源,使得所述蛋白质或其部分保留SMP活性。最好是所述核酸分子编码的蛋白质或其部分保留执行参与谷氨酸棒杆菌的诸如糖的碳水化合物代谢和通过氧化磷酸化过程产生能量分子(例如ATP)的功能的能力。在一个实施方案中,由所述核酸分子编码的蛋白质与本发明氨基酸序列(例如选自具有序列表偶数SEQ ID NO的完整的氨基酸序列)的同源性为至少约至50%、优选至少约60%、更优选至少约70%、80%或90%、最优选至少约95%、96%、97%、98%或99%或更高。在另一优选实施方案中,所述蛋白质是与本发明完整氨基酸序列(由序列表相应的奇数SEQ ID NO(如SEQ ID NO:1、SEQ ID NO:3、SEQ ID NO:5、SEQ ID NO:7…)中所示的可读框编码)基本上同源的全长谷氨酸棒杆菌蛋白质。
在另一优选实施方案中,所述分离的核酸分子得自谷氨酸棒杆菌,并且编码包括一个生物活性结构域的蛋白质(例如SMP融合蛋白),所述生物活性结构域与一种本发明的氨基酸序列(例如序列表中的一种偶数SEQ ID NO序列)有至少约50%或更高的同源性,并且能够执行参与谷氨酸棒杆菌诸如糖的碳水化合物代谢和通过氧化磷酸化过程产生能量分子(例如ATP)的功能,或具有表1给出的一种或多种活性,所述生物活性结构域也包括编码异源多肽或调节区的异源核酸序列。
在另一实施方案中,所述分离的核酸分子至少长15个核苷酸,并且在严格条件下与包含本发明核酸序列(例如序列表奇数SEQ ID NO序列)的核酸分子杂交。优选所述分离的核酸分子相当于天然存在的核酸分子。更优选所述分离的核酸编码天然存在的谷氨酸棒杆菌SMP蛋白或其生物活性部分。
本发明的另一方面涉及含有本发明核酸分子的载体,例如重组表达载体,涉及已经导入这类载体的宿主细胞。在一个实施方案中,这样一种宿主细胞用来通过在合适的培养基中培养所述宿主细胞而生产SMP蛋白。然后从所述培养基或所述宿主细胞中分离出所述SMP蛋白。
本发明的再一方面涉及其中已经导入SMP基因或改变SMP基因的遗传改变的微生物。在一个实施方案中,通过导入作为转基因的编码野生型或突变型SMP序列的本发明核酸分子,已经改变了所述微生物的基因组。在另一实施方案中,所述微生物基因组内的内源SMP基因通过与改变的SMP基因同源重组而已经被改变,例如功能性断裂。在另一实施方案中,微生物中内源的或所引入的SMP基因通过一个或多个点突变、缺失或倒位而被改变,但仍编码功能性SMP蛋白。在再一实施方案中,微生物中SMP基因的一个或多个调节区(例如启动子、阻抑蛋白或诱导物)已经被改变(例如通过缺失、截短、倒位或点突变),使得所述SMP基因的表达得到调节。在一个优选实施方案,所述微生物属于棒杆菌属或短杆菌属,特别优选谷氨酸棒杆菌。在一个优选实施方案中,所述微生物也用于生产需要化合物,例如氨基酸,特别优选赖氨酸。
另一方面,本发明提供一种鉴定受试者中是否存在白喉棒杆菌或其活动情况的方法。该方法包括检测受试者体内的一种或多种本发明的核酸序列或氨基酸序列(例如序列表SEQ ID NO 1-782序列),由此检测所述受试者体内是否存在白喉棒杆菌或其活动情况。
本发明的又一方面涉及分离的SMP蛋白或其部分,例如生物活性部分。在一个优选实施方案中,所述分离SMP蛋白或其部分能够执行参与谷氨酸棒杆菌诸如糖的碳水化合物代谢和通过氧化磷酸化过程产生能量分子(例如ATP)的功能。在另一优选实施方案中,所述分离的SMP蛋白或其部分与本发明的氨基酸序列(例如序列表偶数SEQ IDNO序列)足够同源,使得所述蛋白质或其部分保留执行参与谷氨酸棒杆菌诸如糖的碳水化合物代谢和通过氧化磷酸化过程产生能量分子(例如ATP)的功能的能力。
本发明也提供SMP蛋白的分离制剂。在优选实施方案中,所述SMP蛋白包含本发明的氨基酸序列(例如序列表偶数SEQ ID NO序列)。在另一优选实施方案中,本发明涉及与本发明的完整氨基酸序列(例如序列表偶数SEQ ID NO序列)(由序列表中相应的奇数SEQ IDNO可读框编码)基本上同源的分离的全长蛋白质。在再一实施方案中,所述蛋白质与本发明的完整氨基酸序列(例如序列表偶数SEQ IDNO序列)的同源性为至少约至50%、优选至少约60%、更优选至少约70%、80%或90%、最优选至少约95%、96%、97%、98%或99%或更高。在其它实施方案中,所述分离的SMP蛋白包含与本发明的一种氨基酸序列(例如序列表偶数SEQ ID NO序列)的同源性至少约50%或更高的氨基酸序列,并且能够执行参与谷氨酸棒杆菌诸如糖的碳水化合物代谢和通过氧化磷酸化过程产生能量分子(例如ATP)的功能,或具有表1给出的一种或多种活性。
或者,所述分离的SMP蛋白可以包含这样的核苷酸序列编码的氨基酸序列:所述核苷酸序列在严格条件下与序列表中一种奇数SEQ IDNO核苷酸序列杂交,或与序列表中一种奇数SEQ ID NO核苷酸序列的同源性至少约至50%、优选至少约60%、更优选至少约70%、80%或90%、最优选至少约95%、96%、97%、98%或99%或更高。也优选SMP蛋白的优选形式也具有本文所述一种或多种SMP生物活性。
所述SMP多肽或其生物活性部分可以与一种非SMP多肽有效连接,形成融合蛋白。在优选实施方案中,这种融合蛋白具有不同于单独的所述SMP蛋白的活性。在其它优选实施方案中,这种融合蛋白执行参与谷氨酸棒杆菌诸如糖的碳水化合物代谢和通过氧化磷酸化过程产生能量分子(例如ATP)的功能。在特别优选的实施方案中,这种融合蛋白整合到宿主细胞中,调节所述细胞的需要化合物产生。
另一方面,本发明提供用于筛选调节SMP蛋白活性的分子的方法,所述分子通过与SMP蛋白本身相互作用,或者通过与所述SMP蛋白的底物或结合配偶体相互作用,或者通过调节本发明SMP核酸分子的转录或翻译,调节SMP蛋白活性。
本发明的另一方面涉及用于生产精细化学品的方法。该方法包括培养包含指导本发明SMP核酸分子表达的载体的细胞,使得可生产精细化学品。在一个优选实施方案中,该方法还包括获得含这种载体的细胞的步骤,其中用指导SMP核酸表达的载体转染细胞。在另一优选实施方案中,该方法还包括从培养物中回收所述精细化学品的步骤。在一个特别优选的实施方案中,所述细胞得自棒杆菌属或短杆菌属,或选自表3中所述的菌株。
本发明的另一方面涉及用于调节由微生物生产分子的方法。这类方法包括使所述细胞与调节SMP蛋白活性或SMP蛋白表达的因子接触,使得细胞相关活性相对于在缺乏所述因子时的所述活性而言被改变。在一个优选实施方案中,调节所述细胞的一种或多种谷氨酸棒杆菌碳代谢途径,或调节所述细胞通过诸如氧化磷酸化的过程产生能量,使得改进这种微生物生产需要精细化学品的产率或产率。调节SMP蛋白活性的因子可以是刺激SMP蛋白活性或SMP核酸表达的因子。刺激SMP蛋白活性或SMP核酸表达的因子的实例包括小分子、活性SMP蛋白和已导入所述细胞的编码SMP蛋白的核酸。抑制SMP活性或表达的因子的实例包括小分子和反义SMP核酸分子。
本发明的另一方面涉及调节细胞的需要化合物产率的方法,所述方法包括将野生型或突变型SMP基因导入细胞中,或者保留在单独的质粒上或整合到宿主细胞的基因组中。如果整合到基因组中,则这种整合可以是随机的,或它可以通过同源重组而发生,使得所述天然基因被所导入的拷贝取代,导致所调节的细胞产生所述需要化合物。在一个优选实施方案中,所述产率提高。在另一优选实施方案中,所述化学品是一种精细化学品。在一个特别优选的实施方案中,所述精细化学品是一种氨基酸。在一个尤其优选的实施方案中,所述氨基酸是L-赖氨酸。发明详述
本发明提供SMP核酸分子和SMP蛋白分子,所述分子参与谷氨酸棒杆菌诸如糖的碳化合物代谢和通过氧化磷酸化过程产生能量分子。本发明的分子可以用于调节微生物例如谷氨酸棒杆菌产生精细化学品,这可以产生直接影响(例如过量表达或优化糖酵解途径蛋白对改良谷氨酸棒杆菌的例如丙酮酸的产率、产量和/或生产效率有直接影响),或可以产生间接影响,间接影响仍然使需要化合物的产率、产量和/或生产效率提高(例如调节参与氧化磷酸化的蛋白导致供细胞完成必要代谢过程和其它细胞功能(如核酸和蛋白质生物合成以及转录和翻译)的能量改变)。以下进一步详细说明本发明的各个方面。I.精细化学品
术语“精细化学品”是本领域公知的,包括由生物产生的在各种工业(例如但不限于制药业、农业和化妆品工业)上具有多种应用的分子。这类化合物包括有机酸,例如酒石酸、衣康酸和二氨基庚二酸;蛋白质生成的和非形成蛋白质的氨基酸;嘌呤和嘧啶碱基、核苷和核苷酸(如描述于例如Kuninaka,A.(1996)核苷酸和相关化合物,载于Biotechnology,第6卷,第561-612页,Rehm等编著,VCH:Weinheim以及其中含有的参考文献);脂质、饱和和不饱和脂肪酸(例如花生四烯酸);二元醇(例如丙二醇和丁二醇);糖类(例如透明质酸和海藻糖);芳族化合物(例如芳香胺、香草醛和靛蓝);维生素和辅因子(如描述于Ullmann’s Encyclopedia of Industrial Chemistry,第A27卷,“维生素”,第443-613页(1996)VCH:Weinheim以及其中的参考文献;和Ong,A.S.,Niki,E.和Packer,L.(1995)“Nutrition,Lipids,Health,and Disease”Proceedings of the UNESCO/Confederation of Scientific and TechnologicalAssociations in Malaysia,and the Society for Free Radical Research-Asia,于1994年9月1-3日在马来西亚Penang举行,AOCS Press,(1995));酶;聚酮化合物(Cane等(1998)Science 282:63-68);和Gutcho(1983)Chemicals by Fermentation,Noyes Data Corporation,ISBN:0818805086以及其中的参考文献介绍的所有其它化合物。以下进一步详细描述这些精细化学品中的某些的代谢和应用。A.氨基酸代谢和应用
氨基酸是所有蛋白质的基本结构单元,因此是所有生物正常细胞功能必不可少的。术语“氨基酸”是本领域已知的。形成蛋白质的氨基酸有20种,用作蛋白质的结构单元,在蛋白质中它们通过肽键连接;而非形成蛋白质的氨基酸(已知数百种所述氨基酸)通常不存在于蛋白质中(参见Ulmann’s Encyclopedia of Industrial Chemitry,第A2卷,第57-97页VCH:Weinheim(1985))。氨基酸可以具有D-或L-光学构型,虽然L-氨基酸一般是天然存在的蛋白质中发现的唯一类型。20种形成蛋白质的氨基酸在原核细胞和真核细胞中的各生物合成和降解途径已充分表征(参见例如Stryer,L.Biochemistry,第3版,第578-590页(1988))。“必需”氨基酸(组氨酸、异亮氨酸、亮氨酸、赖氨酸、甲硫氨酸、苯丙氨酸、苏氨酸、色氨酸和缬氨酸)如此命名,是因为它们的生物合成复杂,一般是营养上需要的,所述“必需”氨基酸容易通过简单的生物合成途径转化为其余11种“非必需”氨基酸(丙氨酸、精氨酸、天冬酰胺、天冬氨酸、半胱氨酸、谷氨酸、谷氨酰胺、甘氨酸、脯氨酸、丝氨酸和酪氨酸)。高等动物的确保留了合成这些氨基酸中的某些氨基酸的能力,但必需氨基酸必需从饮食中供应,以便进行正常的蛋白质合成。
除了这些氨基酸在蛋白质生物合成中的功能外,这些氨基酸自身还是令人感兴趣的化学品,已经发现其中许多氨基酸在食品工业、饲料工业、化学工业、化妆品工业、农业和制药业中具有各种用途。赖氨酸不仅是人类营养的重要氨基酸,而且也是单胃动物(例如家禽和猪)营养的重要氨基酸。谷氨酸最常用作调味添加剂(谷氨酸一钠,MSG),并且在食品工业广泛使用,天冬氨酸、苯丙氨酸、甘氨酸和半胱氨酸也是如此。甘氨酸、L-甲硫氨酸和色氨酸都用于制药业。谷氨酰胺、缬氨酸、亮氨酸、异亮氨酸、组氨酸、精氨酸、脯氨酸、丝氨酸和丙氨酸在制药业和化妆品工业上有价值。苏氨酸、色氨酸和D/L-甲硫氨酸是常用的饲料添加剂。(Leuchtenberger,W.(1996)氨基酸-生产技术和应用,载于Rehm等(编著)Biotechnology,第6卷,第14a章,第466-502页,VCH:Weinheim)。另外,已经发现这些氨基酸可用作合成合成氨基酸和蛋白质的前体,例如N-乙酰半胱氨酸、S-羧甲基-L-半胱氨酸、(S)-5-羟基色氨酸以及Ulmann’s Encyclopedia of IndustrialChemistry,第A2卷,第57-97页,VCH:Weinheim,1985中介绍的其它合成氨基酸和蛋白质。
已经充分表征了这些氨基酸在能够生产这些天然氨基酸的生物(例如细菌)中的生物合成(有关细菌氨基酸生物合成及其调节的综述参见Umbarger,H.E.(1978)Ann.Rev.Biochem.47:533-606)。通过柠檬酸循环中的中间体α-酮戊二酸的还原性胺化合成谷氨酸。随后由谷氨酸分别产生谷氨酰胺、脯氨酸和精氨酸。丝氨酸的生物合成是一个三步骤过程,以3-磷酸甘油酸(糖酵解中间体)开始,在氧化、转氨基作用和水解步骤后,产生这种氨基酸。半胱氨酸和甘氨酸都由丝氨酸产生,前者通过高半胱氨酸和丝氨酸缩合而产生,而后者通过在一个由丝氨酸转羟甲基酶催化的反应中将侧链β-碳原子转移至四氢叶酸而产生。苯丙氨酸和酪氨酸由糖酵解和戊糖磷酸途径前体4-磷酸赤藓糖和磷酸烯醇丙酮酸开始的9步骤生物合成途径合成,这两种氨基酸的生物合成途径反在合成预苯酸后的最后两个步骤不同。色氨酸也由这两种起始分子产生,但其合成是一个11步骤途径。酪氨酸也可以在一个由苯丙氨酸羟化酶催化的反应中由苯丙氨酸合成。丙氨酸、缬氨酸和亮氨酸都是糖酵解终产物-丙酮酸的生物合成产物。天冬氨酸由柠檬酸循环的中间体草酰乙酸生成。天冬酰胺、甲硫氨酸、苏氨酸和赖氨酸都是通过天冬氨酸的转化而产生。异亮氨酸由苏氨酸生成。一个复杂的9步骤途径由一种活化糖即5-磷酸核糖-1-焦磷酸产生组氨酸。
超出细胞蛋白质合成需求的氨基酸不能被贮存,而是被降解,为细胞的主要代谢途径提供中间体(有关综述参见Stryer,L.Biochemistry,第3版,第21章,“氨基酸降解和尿素循环”,第495-516页(1988))。虽然细胞能够将不需要的氨基酸转化为有用的代谢中间体,但就能量、前体分子以及合成氨基酸所必需的酶而言,氨基酸生产是昂贵的。因此,氨基酸生物合成受反馈抑制是不奇怪的,在反馈抑制中,特定氨基酸的存在起减慢或完全终止其自身产生的作用(有关氨基酸生物合成途径中的反馈机制的综述,参见Stryer,L.Biochemistry,第3版,第24章,“氨基酸和血红素的生物合成”,第575-600页(1988))。因此,任何特定氨基酸的输出受细胞中存在的氨基酸量的限制。B.维生素、辅因子和营养药的代谢和应用
维生素、辅因子和营养药构成高等动物已经丧失合成能力并且因此必须摄入的另外一组分子,但是它们容易由其它生物例如细菌合成。这些分子或者本身是生物活性物质,或者是可以用作用作电子载体或多种代谢途径的中间体的生物活性物质的前体。这些化合物除了具有营养价值外,也具有作为着色剂、抗氧化剂和催化剂或其它加工助剂的重要工业价值。(有关这些化合物的结构、活性和工业应用的概述,参见例如Ullman’s Encyclopedia of Industrial Chemistry,“维生素”,第A27卷,第443-613页,VCH:Weinheim,1996)。术语“维生素”是本领域公知的,包括生物正常功能需要、但生物自身不能合成的营养物。维生素类可以包括辅因子和营养药化合物。用语“辅因子”包括发生正常酶活性所需的非蛋白性化合物。这类化合物可以是有机化合物或无机化合物;本发明的辅因子分子最好是有机分子。术语“营养药”包括在植物和动物、特别是人类中具有健康益处的食品添加剂。这类分子的实例是维生素、抗氧化剂以及某些脂质(例如多不饱和脂肪酸)。
已经大量表征了这些分子在能够生产其的生物例如细菌中的生物合成(Ullman’s Encyclopedia of Industrial Chemistry,第A27卷,“维生素”第443-613页,VCH:Weinheim,1996;Michal,G.(1999)Biochemical Pathways:An Atlas of Biochemistry and Molecular Biology,John Wiley & Sons;Ong,A.S.,Niki,E.和Packer,L.(1995)“Nutrition,Lipids,Health,and Disease”Proceedings of the UNESCO/Confederationof Scientific and Technological Associations in Malaysia,and the Societyfor Free Radical Research-Asia,于1994年9月1-3日在马来西亚Penang举行,AOCS Press:Champaign,IL X,374 S)。
通过将嘧啶和噻唑部分化学偶联,产生硫胺素(维生素B1)。核黄素(维生素B2)由鸟苷-5’-三磷酸(GTP)和核糖-5’-磷酸合成。核黄素进而用来合成黄素单核苷酸(FMN)和黄素腺嘌呤二核苷酸(FAD)。统称为“维生素B6”的化合物家族(例如吡哆醇、吡哆胺、吡哆醛-5’-磷酸以及商业上使用的盐酸吡哆醇)都是具有共同结构单元5-羟基-6-甲基吡啶的衍生物。泛酸((R)-(+)-N-(2,4-二羟基-3,3-二甲基-1-氧代丁基)-β-丙氨酸)可以通过化学合成或者通过发酵生产。泛酸生物合成中的最后的步骤由ATP驱动的β-丙氨酸和泛解酸缩合组成。负责转化为泛解酸、β-丙氨酸和缩合为泛酸的生物合成步骤的酶是已知的。代谢活性形式的泛酸是辅酶A,其生物合成以5个酶促步骤进行。泛酸、吡哆醛-5’-磷酸、半胱氨酸和ATP是辅酶A的前体。这些酶不仅催化泛酸的生成,而且催化(R)-泛解酸、(R)-pantolacton、(R)-泛醇(前维生素B5)、泛酰巯基乙胺(及其衍生物)和辅酶A的产生。
已经详细研究了微生物中由前体分子庚二酰辅酶A开始的生物素生物合成,并且已经鉴定出所涉及的若干基因。已经发现许多相应的蛋白质也参与铁簇合成,并且是nifS类蛋白质的成员。硫辛酸衍生自辛酸,用作能量代谢中的辅酶,在能量代谢中它成为丙酮酸脱氢酶复合体和α-酮戊二酸脱氢酶复合体的一部分。叶酸类是一类叶酸衍生物的物质,叶酸又衍生自L-谷氨酸、对氨基苯甲酸和6-甲基蝶呤。已经在某些微生物中详细地研究了叶酸及其衍生物的生物合成,所述生物合成起始于代谢中间体鸟苷-5’-三磷酸(GTP)、L-谷氨酸和对氨基苯甲酸。
类咕啉(例如钴胺素、特别是维生素B12)和卟啉属于一类特征为四吡咯环系的化学物质。维生素B12的生物合成足够复杂,以致尚未被完全鉴定,但现在已知许多所涉及的酶和底物。烟酸和烟酰胺是吡啶衍生物,也称为“尼克酸”。烟酸是重要的辅酶NAD(烟酰胺腺嘌呤二核苷酸)和NADP(烟酰胺腺嘌呤二核苷酸磷酸)及其还原形式的前体。
这些化合物的大规模生产一直主要依靠无细胞化学合成,尽管这些化学品中的某些也已经通过大规模微生物培养来生产,例如核黄素、维生素B6、泛酸和生物素。仅维生素B12由于其合成的复杂性通过发酵生产。体外方法花费大量原料和时间,通常费用巨大。C.嘌呤、嘧啶、核苷和核苷酸的代谢和应用
嘌呤和嘧啶代谢基因及其相应的蛋白质是肿瘤疾病和病毒感染的重要的治疗靶。用语“嘌呤”或“嘧啶”包括含氮碱基,它们是核酸、辅酶和核苷酸的组分。术语“核苷酸”包括核酸分子的基本结构单元,它们由一个含氮碱基、一个戊糖(在RNA的情况下,所述糖是核糖;在DNA的情况下,所述糖是D-脱氧核糖)和磷酸构成。用语“核苷”包括用作核苷酸前体、但缺乏核苷酸所具有的磷酸部分的分子。通过抑制这些分子的生物合成或抑制其形成核酸分子的转化,有可能抑制RNA和DNA的合成;通过以靶向癌细胞的方式抑制这种活性,可以抑制肿瘤细胞分裂和复制的能力。另外,有不形成核酸分子、而是用作能量贮存(即AMP)或用作辅酶(即FAD和NAD)的核苷酸。
几个出版物已经描述了通过影响嘌呤和/或嘧啶代谢,将这些化学物质用于这些医学适应征(例如Christopherson,R.I.和Lyons,S.D.(1990)“作为化疗药的嘧啶和嘌呤从头生物合成的有效抑制剂”,Med.Res.Reviews 10:505-548)。对参与嘌呤和嘧啶代谢的酶的研究一直集中于可以用作例如用作免疫抑制剂或抗增殖药的新药的开发(Smith,J.L.,(1995)“核苷酸合成中的酶”,Curr.Opin.Struct.Biol.5:752-757;(1995)Biochem Soc.Transact.23:877-902)。然而,嘌呤和嘧啶碱基、核苷和核苷酸具有其它用途:用作几种精细化学品(例如硫胺素、S-腺苷-甲硫氨酸、叶酸或核黄素)生物合成的中间体、用作细胞的能量载体(例如ATP或GTP)和用于化学品自身,通常用作增味剂(例如IMP或GMP)或用于若干医药用途(参见例如Kuninaka,A.(1996)Nucleotides andRelated Compounds in Biotechnology,第6卷,Rehm等编著,VCH:Weinheim,第561-612页)。此外,参与嘌呤、嘧啶、核苷或核苷酸代谢的酶越来越多地用作开发用于保护作物的化学品的靶,所述化学品包括杀真菌剂、除草剂和杀虫剂。
已经鉴定了这些化合物在细菌中的代谢(有关综述参见例如Zalkin,H.和Dixon,J.E.(1992)“嘌呤核苷酸的从头生物合成”,载于:Progress in Nucleic Acid Research and Molecular Biology,第42卷,Academic Press,第259-287页;和Michal,G.(1999)“核苷酸和核苷”,载于:Biochemical Pathways:An Atlas of Biochemistry and MolecularBiology,第8章,Wiley:纽约)。嘌呤代谢一直是广泛研究的主题,并且对于细胞的正常功能是必不可少的。在高等动物中嘌呤代谢受损可以引起严重的疾病,例如痛风。嘌呤核苷酸由核糖-5-磷酸开始合成,通过中间体化合物肌苷-5’-磷酸(IMP)的一系列步骤,导致鸟苷-5’-一磷酸(GMP)或腺苷-5’-一磷酸(AMP)的产生,由GMP或AMP容易生成用作核苷酸的三磷酸形式。这些化合物也用作能量贮存物,因此其降解为细胞中的许多不同生物化学过程提供能量。嘧啶生物合成通过由核糖-5-磷酸形成尿嘧啶-5’-一磷酸(UMP)而进行。UMP进而转化为胞苷-5’-三磷酸(CTP)。所有这些核苷酸的脱氧形式在一步还原反应中产生,从所述核苷酸的二磷酸核糖形式还原反应为所述核苷酸的二磷酸脱氧核糖形式。在磷酸化后,这些分子能够参与DNA合成。D.海藻糖的代谢和应用
海藻糖由以α,α-1,1-连接的两分子葡萄糖组成。海藻糖在食品工业中通常用作甜味剂,即用于干燥食品或冷冻食品和饮料中的一种添加剂。然而,它在制药业、化妆品工业和生物技术工业中也具有许多应用(参见例如Nishimoto等,(1998)美国专利号5,759,610;Singer,M.A.和Lindquist,S.(1998)Trends Biotech.16:460-467;Paiva,C.L.A.和Panek,A.D.(1996)Biotech.Ann.Rev.2:293-314;和Shiosaka,M.(1997)J.Japan 172:97-102)。海藻糖由许多微生物通过酶产生,并且天然释放到周围介质中,可以用本领域已知的方法从所述周围介质中收集海藻糖。II.糖和碳分子的利用以及氧化磷酸化
碳是形成所有有机化合物的重要元素,因此它不仅是谷氨酸棒杆菌生长分裂的营养要求,而且也是该微生物过量产生精细化学品的营养要求。诸如单糖、双糖或多糖的糖类是特别优良的碳源,所以标准生长培养基通常含有一种或多种以下糖:葡萄糖、果糖、甘露糖、半乳糖、核糖、山梨糖、核酮糖、乳糖、麦芽糖、蔗糖、棉子糖、淀粉或纤维素(Ullmann’s Encyclopedia of Industrial Chemistry(1987)第A9卷,“酶”,VCH:Weinheim)。另一方面,更多的复合型糖可以用于所述培养基,例如糖蜜或糖精制中的其它副产品。可使用所述糖以外的其它化合物作为替代碳源,包括醇类(例如乙醇或甲醇)、链烷类、糖醇、脂肪酸和有机酸(例如醋酸或乳酸)。关于碳源以及培养微生物对它们的利用的综述,参见:Ullman’s Encyclopedia of Industrial Chemistry(1987)第A9卷,“酶”,VCH:Weinheim;Stoppok,E.和Buchholz,K.(1996)“发酵使用的糖型原料”,载于:Biotechnology(Rehm,H.J.编著)第6卷,VCH:Weinheim,第5-29页;Rehm,H.J.(1980)IndustrielleMikrobioloie,Springer:Berlin;Bartholomew,W.H.和Reiman,H.B.(1979),发酵工艺经济学,载于:Peppler,H.J.和Perlman,D.编著,Microbial Technology第2版,第2卷,第18章,Academic Press:纽约;以及Kockova-Kratachvilova,A.(1981)工业微生物的特征,载于:Rehm,H.J.和Reed,G.编著,Handbook of Biotechnology,第1卷,第1章,Verlag Chemie:Weiheim。
在这些富含能量的碳分子吸收入细胞后,必须进行加工处理,使其能够被一种主要糖代谢途径降解。这种途径直接产生有用的降解产物,例如5-磷酸核糖和磷酸烯醇丙酮酸,磷酸烯醇丙酮酸随后可转化为丙酮酸。3种最重要的细菌糖代谢途径包括Embden-Meyerhoff-Pamas(EMP)途径(也称为糖酵解途径或二磷酸果糖途径)、己糖一磷酸(HMP)途径(也称为戊糖旁路途径或磷酸戊糖途径)和Entner-Doudoroff(ED)途径(综述参见Michal,G.(1999)Biochemical Pathways:An Atlas ofBiochemistry and Molecualr Biology,Wiley:纽约和Stryer,L.(1988)Biochemistry,第13-19章,Freeman:纽约,以及其中引用的参考文献)。
EMP途径使己糖分子转化为丙酮酸,该过程产生2分子ATP和2分子NADH。以葡萄糖-1-磷酸(可以从培养基中直接吸收,或者可以由糖元、淀粉或纤维素产生)为原料,使葡萄糖分子异构化为果糖-6-磷酸,使其磷酸化,裂解成2个三碳分子甘油醛-3-磷酸。在脱氢化、磷酸化和连续重排后,获得丙酮酸。
HMP途径使葡萄糖转化为还原当量物如NADPH,而且产生戊糖和四糖化合物,它们是许多其它代谢途径必须的中间体和前体。在HMP途径中,葡萄糖-6-磷酸通过2个连续脱氢酶反应(它还释放2分子NADPH)和1个羧基化步骤转化为核酮糖-5-磷酸。核酮糖-5-磷酸也可以转化为木酮糖-5-磷酸和核糖-5-磷酸;前者可经过一系列生化步骤转化为葡萄糖-6-磷酸,葡萄糖-6-磷酸可进入EMP途径,而后者通常用作细胞它生物合成途径的中间体。
ED途径以化合物葡萄糖或葡糖酸开始,它随后磷酸化以及脱氢化形成2-脱氢-3-脱氧-6-P-葡糖酸。葡糖醛酸和半乳糖醛酸也可以通过更复杂的生化途径转化为2-脱氢-3-脱氧-6-P-葡糖酸。该产物分子随后裂解为甘油醛-3-P和丙酮酸;甘油醛-3-P本身也可以转化为丙酮酸。
EMP和HMP途径共有许多特征,包括中间体和酶。EMP途径提供ATP量最多,但是它不产生核糖-5-磷酸,核糖-5-磷酸是例如核酸生物合成的重要前体,也不产生赤藓糖-4-磷酸,赤藓糖对氨基酸生物合成是重要的。因此只能利用葡萄糖EMP途径的微生物不能在葡萄糖作为唯一碳源的简单培养基上生长。这种微生物称为苛求菌,其生长需要加入复合有机化合物,例如见于酵母提取物的复合有机化合物。
相反,HMP途径产生核酸和氨基酸生物合成二者必需的全部前体,却只产生EMP途径一半的ATP能量。HMP途径也产生NADPH,NADPH可以用于生物合成途径中的还原反应。然而,HMP途径不直接产生丙酮酸,所以,这些微生物还必须具有EMP途径的这一部分。所以,无数微生物、尤其是兼性厌氧菌已经进化而具有这两种途径,这不是意外的。
ED途径目前只见于细菌。尽管该途径在前体形成的反方向上与人HM途径部分有关,但是ED途径通过醛缩酶裂解3-酮基脱氧-6-磷酸葡糖酸直接形成丙酮酸盐。ED途径可以独立存在,为大多数严格厌氧微生物所利用。最终结果类似于HMP途径,但是只有当碳原子转化为丙酮酸盐而不是前体分子时,才产生1摩尔ATP。
通过以上任一途径产生的丙酮酸分子能够很容易地通过Krebs循环(也称为柠檬酸循环、柠檬酸循环或三羧酸循环(TCA循环))转化为能量。在Krebs循环中,首先丙酮酸脱羧,产生1分子NADH、1分子乙酰CoA和1分子二氧化碳。乙酰CoA的乙酰基随后与4碳单位的草酰乙酸(oxaolacetate)反应,形成6碳有机酸柠檬酸。脱水而且再释放2分子二氧化碳。最后,又产生草酰乙酸而且可以再用作乙酰基受体,至此完成循环。TCA循环中的中间体氧化释放的电子转移给NAD+,产生NADH。
呼吸过程中,NADH电子转移给氧分子或其它终末电子受体。该过程由呼吸链催化,呼吸链是包含膜内在蛋白和膜结合蛋白的电子传递体系。该系统有两个基本作用:第一,接受电子供体的电子并将其转移给电子受体,第二,通过合成ATP保存电子转移释放的部分能量。已知若干种氧化还原酶和电子转运蛋白参与该过程,包括NADH脱氢酶、含黄素电子载体、铁硫蛋白和细胞色素。NADH脱氢酶位于质膜的胞质面,它将氢原子从NADH转移给黄素蛋白,然后接受NADH的电子。黄素蛋白是一组具有黄素辅基的电子载体,当它接受和转移电子时交替还原和氧化。已知3种黄素参与这些反应:核黄素、黄素-腺嘌呤二核苷酸(FAD)和黄素单核苷酸(FMN)。铁硫蛋白含有一簇铁原子和硫原子,它们不与血红素键合,但是仍然能够参与脱水和再水化反应。琥珀酸脱氢酶和顺乌头酸酶是典型的铁硫蛋白;其铁硫复合物作为整个电子传递链的组成部分起接受和转移电子的作用。细胞色素是含有铁卟啉环(血红素)的蛋白。有许多不同类型的细胞色素,它们的还原电位不同。在功能上,这些细胞色素构成其中电子可以转移给正还原电位不断递增的其它细胞色素的途径。已知的其它类型非蛋白电子载体有:脂溶性醌(例如辅酶Q)。这些分子也起氢原子受体和电子供体的作用。
呼吸链发挥作用产生跨细胞膜质子梯度,产生质子动力。细胞利用这种力通过跨膜酶ATP合酶合成ATP。ATP酶是一种多蛋白的复合物,其中
分子经细胞膜转运使细胞内亚单位物理性旋转,伴随着ADP磷酸化形成ATP(综述参见Fillingame,R.H.和Divall,S.(1999)Novartis Found.Symp.221:218-229,229-234)。
非己糖碳底物也可以用作细胞的碳源和能量源。这种底物首先转化为葡糖异生途径的己糖,其中细胞首先合成葡萄糖,然后降解产生能量。该反应的原料是磷酸烯醇丙酮酸(PEP),磷酸烯醇丙酮酸是一种糖酵解途径的重要中间体。PEP可以由糖以外的底物形成,例如醋酸,或者通过草酰乙酸(本身为TCA循环的中间体)脱羧形成。逆转糖酵解途径(利用不同于原始糖酵解途径的级联酶)可以形成葡萄糖-6-磷酸。丙酮酸转化为葡萄糖需要利用6个高能磷酸键,而糖酵解将葡萄糖转化为丙酮酸仅产生2个ATP。然而,葡萄糖完全氧化(糖酵解、丙酮酸转化为乙酰CoA、柠檬酸循环和氧化磷酸化)产生36-38个ATP,这样进行葡糖异生净损失的高能磷酸键通过氧化葡萄糖产生这样的高能磷酸键而总体上增加,从而得到补偿。III.本发明的元件和方法
本发明至少部分基于发现了本文称为SMP核酸和蛋白分子的新型分子,所述SMP分子参与谷氨酸棒杆菌的糖转化为有用的降解产物和能量(例如ATP),或者通过其它过程如氧化磷酸化产生有用的富含能量的分子(例如ATP)。在一个实施方案中,SMP分子参与谷氨酸棒杆菌诸如糖的碳化合物代谢和通过诸如氧化磷酸化的过程产生能量分子(例如ATP)。在一个优选实施方案中,参与谷氨酸棒杆菌碳代谢和产生能量的本发明SMP分子活性对该生物产生需要精细化学品产生影响。在一个特别优选的实施方案中,调节本发明SMP分子的活性,使得本发明SMP蛋白参与的谷氨酸棒杆菌代谢途径和能量途径在产率、产量和/或生产效率上受到调节,这直接或者间接调节谷氨酸棒杆菌产生需要精细化学品的产率、产量和/或生产效率。
用语“SMP蛋白”或“SMP多肽”包括能够执行参与谷氨酸棒杆菌诸如糖的碳水化合物代谢和通过诸如氧化磷酸化的过程产生能量的功能的蛋白质。SMP蛋白实例包括由表1给出的SMP基因编码的蛋白质以及由奇数SEQ ID NO编码的蛋白质。术语“SMP基因”或“SMP核酸序列”包括编码SMP蛋白的核酸序列,该核酸序列由一个编码区以及相应的非翻译5’和3’序列区组成。SMP蛋白的实例包括表1给出的蛋白。术语“产量”或“生产能力”是本领域公知的,包括在给定时间和给定发酵体积内获得的发酵产物(例如需要精细化学品)浓度(例如每小时每升的kg产物)。术语“生产效率”包括达到特定生产水平所需的时间(例如细胞达到特定精细化学品产出率需要多长时间)。术语“产率”或“产物/碳产率”是本领域公知的,包括碳源转化为产物(即精细化学品)的效率。这通常表示为例如每kg碳源的kg产物。通过增加所述化合物的产率或产量,可增加该化合物在给定时间、给定量培养物中的回收分子数量或有效回收分子数量。术语“生物合成”或“生物合成途径”是本领域公知的,包括细胞以可能为多步骤、高度调节的过程从中间体化合物开始合成化合物、最好是有机化合物。术语“降解”或“降解途径”是本领域公知的,包括细胞以可能为多步骤、高度调节的过程将化合物、最好是有机化合物分解为降解产物(一般而言,是较小或复杂度较低的分子)。术语“降解产物”是本领域公知的,包括化合物的分解产物。所述产物本身可能在细胞生物合成其它化合物时用作必需的前体(起点)或中间体分子。用语“代谢”是本领域公知的,包括生物体内发生的全部生物化学反应。特定化合物的代谢(例如诸如甘氨酸的氨基酸的代谢)则包括细胞中与该化合物有关的全部生物合成、修饰和降解途径。
在另一实施方案中,本发明的SMP分子能够调节微生物例如谷氨酸棒杆菌中需要分子例如精细化学品的产生。本发明SMP蛋白改变可能通过多种机制直接影响掺入这种改变蛋白的谷氨酸棒杆菌菌株产生精细化学品的产率、产量和/或生产效率。降解高能碳分子如糖和通过氧化磷酸化使化合物如NADH和FADH2转化为更有用的形式可产生许多化合物,这些化合物本身可能就是需要的精细化学品,例如丙酮酸、ATP、NADH和多种中间体糖化合物。进而,细胞可利用这些代谢途径产生的能量分子(如ATP)和还原当量物(如NADH或NADPH)驱动在能量上不利的反应。这样的不利反应包括许多精细化学品生物合成途径。人们通过改进细胞利用特定糖的能力(例如操作编码参与所述细胞降解并转化该糖为能量的酶的基因)可提高能量,以便细胞可进行不利但却是需要的代谢反应(例如生物合成需要精细化学品)
诱变一种或多种本发明SMP基因也可能产生活性改变的SMP蛋白,活性改变的SMP蛋白间接影响谷氨酸棒杆菌产生一种或多种需要精细化学品。例如,人们通过提高利用一种或多种糖的效率(从而增强所述糖转化为有用的能量分子)、或者提高还原当量物转化为有用的能量分子的效率(例如通过提高氧化磷酸化效率或增强ATP合酶活性)能够增加这些高能化合物量,供细胞驱动通常不利的代谢过程。这样的过程包括构建细胞壁、转录、翻译以及生物合成细胞生长分裂必需的化合物(例如核苷酸、氨基酸、维生素、脂质等)(Lengeler等(1999)Biologyof Prokaryotes,Thieme Verlag:Stuttgart,第88-109、913-918、875-899页)。通过改进这些工程细胞的生长和繁殖,既可提高大规模培养物中的细胞存活力,也可以提高其分裂率,这样更大量的细胞能够在发酵罐培养物中存活。至少因为存在更多的产生所需精细化学品的活细胞而提高产率、产量或生产效率。此外,糖代谢产生大量降解和中间体化合物为整个细胞其它生物合成途径的必需前体和中间体。例如许多氨基酸直接由通常产生自糖酵解或TCA循环的化合物合成(例如丝氨酸由糖酵解中间体3-磷酸甘油酸合成)。因而,通过增强了糖转化为有用能量分子的效率,也可以增加有用的降解产物数量。
本发明的分离的核酸序列包含在谷氨酸棒杆菌菌株的基因组中,所述菌株可通过美国典型培养物保藏中心获得,保藏号为ATCC13032。所述分离的谷氨酸棒杆菌SMP DNA的核苷酸序列和谷氨酸棒杆菌SMP蛋白的预测氨基酸序列分别示于序列表奇数SEQ ID NO和偶数SEQ ID NO中。进行计算机分析,将这些核苷酸序列分类和/或鉴定为编码具有参与谷氨酸棒杆菌诸如糖的碳化合物代谢和通过诸如氧化磷酸化的过程产生能量分子的功能的蛋白质的序列。
本发明也涉及所具有的氨基酸与本发明氨基酸序列(例如序列表偶数SEQ ID NO序列)基本上同源的蛋白质。本文所用的所具有的氨基酸序列与选定氨基酸序列基本上同源的蛋白质,与所选定氨基酸序列(例如所述完整的所选定氨基酸序列)至少约50%同源。所具有的氨基酸序列与选定氨基酸序列基本上同源的蛋白质,也可以与所选定氨基酸序列有至少约50-60%、优选至少约60-70%、更优选至少约70-80%、80-90%或90-95%、最优选至少约96%、97%、98%、99%或更高的同源性。
本发明的SMP蛋白或其生物活性部分或片段可以参与谷氨酸棒杆菌诸如糖的碳化合物代谢和通过诸如氧化磷酸化的过程产生能量分子(例如ATP),或具有表1给出的一种或多种活性。
在以下小节中更加详细地描述本发明的各个方面。A.分离的核酸分子
本发明的一个方面涉及编码SMP多肽或其生物活性部分的分离的核酸分子、以及适合用作鉴定或扩增SMP编码核酸(例如SMP DNA)的杂交探针或引物的核酸片段。本文所用的术语“核酸分子”将是指包括DNA分子(例如cDNA或基因组DNA)和RNA分子(例如mRNA)以及采用核苷酸类似物产生的DNA或RNA类似物。该术语也包括位于基因编码区3’端和5’端的非翻译序列:编码区5’端上游的至少约100个核苷酸的序列和基因编码区3’端下游至少约20个核苷酸的序列。所述核酸分子可以是单链或双链,但最好是双链DNA。“分离的”核酸分子是与所述核酸天然来源中存在的其它核酸分子分离的核酸分子。最好是“分离的”核酸分子不含所述核酸来源生物的基因组DNA中天然邻接所述核酸的序列(即位于所述核酸5’端和3’端的序列)。例如,在各种实施方案中,分离的SMP核酸分子可以含有小于约5kb、4kb、3kb、2kb、1kb、0.5kb或0.1kb的在所述核酸来源的细胞(如谷氨酸棒杆菌细胞)的基因组DNA中天然邻接所述核酸分子的核苷酸序列。此外,“分离的”核酸分子,例如DNA分子,可以基本上不含其它细胞物质,或当通过重组技术培养时不含培养基,或当化学合成时不含化学前体或其它化学物质。
本发明的核酸分子(例如具有序列表奇数SEQ ID NO核苷酸序列的核酸分子)或其部分可以用标准生物学技术和本文提供的序列信息来分离。例如,可以用序列表奇数SEQ ID NO序列之一的全部序列或部分序列作为杂交探针,采用用标准杂交技术(例如,描述于以下文献的技术:Sambrook,J.,Fritsh,E.F.和Maniatis,T.Molecular Cloning:ALaboratory Manual.第2版,Cold Spring Harbor Laboratory,Cold SpringHarbor Laboratory Press,Cold Spring Harbor,NY,1989),从谷氨酸棒杆菌文库中分离出谷氨酸棒杆菌SMP DNA。此外,包含本发明核酸序列(例如奇数SEQ ID NO)之一的全部或部分序列的核酸分子,可以采用基于该序列设计的寡核苷酸引物,通过聚合酶链式反应来分离(例如,包含本发明核酸序列之一(如序列表奇数SEQ ID NO)的全部或部分序列的核酸分子可以采用基于所述序列的设计的寡核苷酸引物通过聚合酶链式反应来分离)。例如,可以从正常的内皮细胞分离mRNA(例如通过Chirgwin等(1979)Biochemistry 18:5294-5299的硫氰酸胍抽取法),然后用反转录酶(例如莫洛尼MLV反转录酶,可得自Gibco/BRL,Bethesda,MD;或AMV反转录酶,可得自Seikagaku America,Inc.,St.Petersburg,FL)制备DNA。可以基于序列表中所示的核苷酸序列之一,设计用于聚合酶链式反应扩增的合成寡核苷酸引物。用cDNA或者基因组DNA作为模板,用合适的寡核苷酸引物,按照标准PCR扩增技术,可以扩增本发明的核酸。将如此扩增的核酸克隆到合适的载体中,并且通过DNA序列分析进行鉴定。此外,对应于SMP核苷酸序列的寡核苷酸可以通过标准合成技术,例如用自动DNA合成仪来制备。
在一个优选实施方案中,本发明的分离的核酸分子包含一种序列表中所示的核苷酸序列。序列表所示本发明核酸序列相当于本发明的谷氨酸棒杆菌SMP DNA。该DNA包含编码SMP蛋白的序列(即序列表各奇数SEQ ID NO所示的“编码区”)以及5’非翻译序列和3’非翻译序列,所述非翻译序列也示于序列表奇数SEQ ID NO中。或者,所述核酸分子可以仅包含序列表任一核酸序列的编码区。
为了进行这种应用,人们知道,序列表中所示的每种核酸序列和氨基酸序列具有标识性RXA、RXN或RXS编号,所述编号具有后接5位数字的标识符RXA、RXN或RXS(即RXA01626、RXN00043或RXS0735)。每种所述核酸序列包含至多三个部分:一个5’上游区、一个编码区和一个下游区。这三个区中的每个区通过相同的RXA、RXN或RXS标识符来鉴别,以避免混淆。叙述“序列表奇数序列之一”则是指序列表任一核酸序列,它也可以根据其不同的RXA、RXN或RXS标识符来区分。这些序列中的每个序列的编码区被翻译为相应的氨基酸序列,所述氨基酸序列也示于序列表中,作为紧接相应核酸序列之后的偶数SEQ ID NO。例如,RXA02735的编码区示于SEQ ID NO:1中,而其编码的氨基酸序列示于SEQ ID NO:2中。本发明核酸分子的序列根据与它们所编码的氨基酸分子相同的RXA、RXN或RXS标识符来鉴别,使得可以容易地将它们相联系。例如,名为RXA00042的氨基酸序列是RXA00042核酸分子的核苷酸序列编码区翻译的。RXA、RXN和RXS核苷酸序列和氨基酸序列与其指定的SEQ ID NO之间的对应关系见表1。
本发明的若干基因是“F标志基因”。F标志基因包括表1所示的在RXA标识符之前具有一个“F”的基因。例如,如表1所示称为“F RXA01312”的SEQ ID NO:11是一种F标志基因,SEQ ID NO:29、33和39(在表1中分别称为“F RXA02803”,“F RXA02854”,“FRXA01365”)也是如此。
在一个实施方案中,本发明的核酸分子不包括表2中编辑的核酸分子。就dapD基因而言,该基因的序列公布于Wehrmann,A.等(1998)J.Bacteriol.180(12):3159-3165中。然而,本申请发明人获得的序列显著长于所公布的形式。认为所述公布形式是因为不正确的起始密码子,因此仅代表真实编码区的一个片段。
在另一优选实施方案中,本发明的分离的核酸分子包含一种本发明核苷酸序列(例如序列表奇数SEQ ID NO序列)的互补序列的核酸分子或其部分。与一种本发明核苷酸序列的互补的核酸分子是与序列表中所示的核苷酸序列之一(例如奇数SEQ ID NO:序列)足够互补、使得可以与本发明核苷酸序列之一杂交并因此形成稳定双链体的核酸分子。
在再一优选实施方案中,本发明的分离的核酸分子包含这样的核苷酸序列或其部分:所述核苷酸序列与本发明核苷酸序列(例如序列表奇数SEQ ID NO序列)的同源性为至少约50%、51%、52%、53%、54%、55%、56%、57%、58%、59%或60%,优选至少约61%、62%、63%、64%、65%、66%、67%、68%、69%或70%,更优选至少约71%、72%、73%、74%、75%、76%、77%、78%、79%或80%、81%、82%、83%、84%、85%、86%、87%、88%、89%或90%、或91%、92%、93%、94%,甚至更优选至少约95%、96%、97%、98%、99%或更高。本发明也包括上述数值之间的范围和标识数值(例如70-90%相同或80-95%相同)。例如,包括采用任何组合的上述值作为上限和/或下限的标识值范围。在另一优选实施方案中,本发明的分离的核酸分子包含与本发明的核苷酸序列之一杂交、例如在严格条件下杂交的核苷酸序列或其部分。
此外,本发明的核酸分子可以仅包含序列表中一种奇数SEQ IDNO序列编码区的一部分,例如可以用作探针或引物的片段或编码SMP蛋白的生物活性部分的片段。根据从谷氨酸棒杆菌克隆SMP基因而测定的核苷酸序列,使得可以制备用于鉴定和/或克隆其它细胞类型或生物中的SMP同系物以及其它棒杆菌或相关菌种的SMP同系物的探针或引物。所述探针/引物通常包含基本纯化的寡核苷酸。所述寡核苷酸通常包含一种核苷酸序列区,所述核苷酸序列区在严格条件下与本发明核苷酸序列之一(如序列表奇数SEQ ID NO序列)的有义链、这些序列之一的反义序列或其天然存在的突变体的至少约12个、优选约25个、更优选约40个、50个或75个连续核苷酸杂交。基于本发明核苷酸序列的引物可以用于PCR反应中,以克隆SMP同系物。基于所述SMP核苷酸序列的探针可以用来检测编码同一蛋白或同源蛋白的转录物或基因组序列。在优选实施方案中,所述探针还包含与其连接的一个标记基团,例如所述标记基团可以是放射性同位素、荧光化合物、酶或酶辅因子。这类探针可以用作诊断试验试剂盒的一部分,以鉴定异常表达SMP蛋白的细胞,例如通过测定细胞样品中SMP编码核酸的水平,例如检测SMP mRNA水平或检测基因组SMP基因是否突变或缺失。
在一个实施方案中,本发明的核酸分子编码一种包含与本发明氨基酸序列(例如序列表偶数SEQ ID NO序列)足够同源的氨基酸序列的蛋白质或其部分,使得所述蛋白质或其部分保留参与谷氨酸棒杆菌诸如糖的碳化合物代谢和通过诸如氧化磷酸化的过程产生能量分子(例如ATP)的能力。本文所用的用语“足够同源”是指这样一种蛋白质或其部分:所述蛋白质或其部分所具有的氨基酸序列包含最小数目的与本发明氨基酸序列相同或等同(例如所具有的侧链与序列表偶数SEQID NO序列中的氨基酸残基相似的氨基酸残基)氨基酸残基,使得所述蛋白质或其部分能够参与谷氨酸棒杆菌诸如糖的碳化合物代谢和通过诸如氧化磷酸化的过程产生能量分子(例如ATP)。本文所述的这类糖代谢途径或能量产生系统的蛋白成员可能在一种或多种精细化学品的生产和分泌方面起作用。在本文中也描述了这类活性的实例。因此,“SMP蛋白的功能”直接或者间接地影响一种或多种精细化学品的产率、产量和/或生产效率。SMP蛋白活性实例见表1。
在另一实施方案中,所述蛋白质与本发明的完整氨基酸序列(例如序列表偶数SEQ ID NO序列)的同源性为至少约50-60%,优选至少约60-70%,更优选至少约70-80%、80-90%、90-95%,最优选至少约96%、97%、98%、99%或更高。
本发明SMP核酸分子所编码的蛋白质的部分最好是一种所述SMP蛋白的生物活性部分。本文所用的术语“SMP蛋白的生物活性部分”包括参与谷氨酸棒杆菌诸如糖的碳化合物代谢和产能途径的SMP蛋白的部分,例如结构域/基序,或具有表1给出的一种活性。为了确定SMP蛋白或其生物活性部分是否可以参与谷氨酸棒杆菌碳化合物代谢和产生富含能量的分子,可以进行酶活性的测定。这类测定方法是本领域技术人员众所周知的,如实施例部分的实施例8中详细描述的。
如下制备其它编码SMP蛋白生物活性部分的核酸片段:分离一种本发明氨基酸序列(例如序列表偶数SEQ ID NO序列)的一部分,表达所述SMP蛋白或肽的编码部分(例如通过体外重组表达),并且评价所述SMP蛋白或肽的编码部分的活性。
本发明还包括由于遗传密码的简并性而不同于本发明一种核苷酸序列(例如序列表奇数SEQ ID NO序列)(和其部分)并因此编码与本发明核苷酸序列所编码蛋白相同的SMP蛋白的核酸分子。在另一实施方案中,本发明的分离的核酸分子所具有的核苷酸序列编码具有序列表中所示氨基酸序列(例如偶数SEQ ID NO)的蛋白质。在再一实施方案中,本发明的核酸分子编码与本发明氨基酸序列(由序列表奇数SEQID NO中所示的可读框编码)基本上同源的全长谷氨酸棒杆菌蛋白。
本领域技术人员知道,在一个实施方案中,本发明序列不包括现有技术的序列,例如在本发明之前就获得的表2或4所示的Genbank序列。在一个实施方案中,本发明包括与本发明的核苷酸序列或氨基酸序列具有的同一性百分比大于现有技术的序列(例如表2或4中叙述的Genbank序列(或由这种序列编码的蛋白质))的同一性百分比的核苷酸序列和氨基酸序列。例如,本发明包括:与名为RXA00014(SEQ IDNO:41)的核苷酸序列的同一性大于58%和/或至少为58%的核苷酸序列;与名为RXA00195(SEQ ID NO:399)的核苷酸序列的同一性大于%和/或至少为%的核苷酸序列;以及与名为RXA00196(SEQ ID NO:401)的核苷酸序列的同一性大于42%和/或至少为42%的核苷酸序列。本领域技术人员通过检测任何给定的本发明序列三个最高命中每个的表4所示的GAP计算的同一性百分比分数,并且用100%减去最高GAP计算的同一性百分比,能够计算出所述给定的本发明序列同一性百分比的下限。本领域技术人员也会知道,本发明也包括同一性百分比大于如此计算的下限(例如至少50%、51%、52%、53%、54%、55%、56%、57%、58%、59%或60%,优选至少约61%、62%、63%、64%、65%、66%、67%、68%、69%或70%,更优选至少约71%、72%、73%、74%、75%、76%、77%、78%、79%或80%、81%、82%、83%、84%、85%、86%、87%、88%、89%或90%、或91%、92%、93%、94%,甚至更优选至少约95%、96%、97%、98%、99%或更高)的核酸序列和氨基酸序列。
除了序列表奇数SEQ ID NO中所示的谷氨酸棒杆菌SMP核苷酸序列外,本领域技术人员知道,在群体(例如谷氨酸棒杆菌群体)中可能存在导致SMP蛋白氨基酸序列改变的DNA序列多态性。由于自然变异,这类所述SMP基因中的遗传多态性可能存在于群体的个体中。本文所用的术语“基因”和“重组基因”是指包含编码SMP蛋白、最好是谷氨酸棒杆菌SMP蛋白的可读框的核酸分子。这类自然变异通常导致所述SMP基因核苷酸序列中1-5%的变异。由于自然变异的结果并且不改变SMP蛋白功能活性的任何或所有这类核苷酸变异和所产生的氨基酸多态性包括在本发明范围内。
根据相当于本发明谷氨酸棒杆菌SMP DNA的天然变异体和非谷氨酸棒杆菌同系物的核酸分子与本文所公开的谷氨酸棒杆菌SMP核酸的同源性,采用所述谷氨酸棒杆菌DNA或其部分作为杂交探针在严格杂交条件下按照标准杂交技术,可以分离出所述核酸分子。因此,在另一实施方案中,本发明的分离的核酸分子至少15个核苷酸长,并且在严格条件下与包含序列表奇数SEQ ID NO核苷酸序列的核酸分子杂交。在一个实施方案中,所述核酸的长度至少为30个、50个、100个、250个或更多个核苷酸。本文所用的术语“在严格条件下杂交”意在描述相互之间至少60%同源的核苷酸序列通常保留相互杂交的杂交和洗涤条件。最好是所述条件使得相互之间同源性为至少约65%、更优选至少约70%、甚至更优选至少约75%或更高的序列通常保持相互杂交。这类严格条件是本领域技术人员已知的,并且可以在Ausubel等,Current Protocols in Molecular Biology,John Wiley & Sons,N.Y.(1989),6.3.1-6.3.6中找到。严格杂交条件的一个优选的非限制性实例是在6X氯化钠/柠檬酸钠(SSC)中于约45℃杂交,然后在0.2 X SSC,0.1% SDS中于50-65℃进行一次或多次洗涤。最好是在严格条件下与本发明核苷酸序列杂交的本发明的分离核酸分子相当于天然存在的核酸分子。本文所用的“天然存在的”核酸分子是指具有天然存在的核苷酸序列(例如编码天然蛋白质)的RNA或DNA分子。在一个实施方案中,所述核酸编码一种天然谷氨酸棒杆菌SMP蛋白。
除了在群体中可能存在的所述SMP序列的天然存在的变异体外,本领域技术人员还知道,可以通过在本发明核苷酸序列中进行突变而导入改变,由此导致所编码SMP蛋白的氨基酸序列改变,而不改变所述SMP蛋白的功能能力。例如,可以在本发明核苷酸中进行导致在“非必需”氨基酸残基上氨基酸取代的核苷酸取代。“非必需”氨基酸残基是可以在所述SMP蛋白的野生型序列(例如序列表偶数SEQID NO)中被改变、而不改变所述SMP蛋白活性的残基,而“必需”氨基酸残基是SMP蛋白活性需要的。然而,其它氨基酸残基(例如在具有SMP活性的结构域中不保守或仅半保守的残基)可能不是活性必需的,因此可能适合改变而不改变SMP活性。
因此,本发明的另一方面涉及编码SMP蛋白的核酸分子,所述SMP蛋白的SMP活性非必需氨基酸残基改变。这类SMP蛋白在氨基酸序列上不同于序列表偶数SEQ ID NO序列,但仍保留至少一种本文所述的SMP活性。在一个实施方案中,所述分离的核酸分子包含编码蛋白质的核苷酸序列,其中所述蛋白质包含与本发明氨基酸序列至少约50%同源的氨基酸序列,并且能够参与谷氨酸棒杆菌诸如糖的碳化合物代谢和生物合成高能化合物,或具有表1给出的一种或多种活性。最好是所述核酸分子编码的蛋白质与序列表中一种偶数SEQ IDNO氨基酸序列至少约50-60%同源,更优选与这些序列之一至少约60-70%同源,更优选与这些序列之一至少约70-80%、80-90%、90-95%同源,最优选与一种本发明的氨基酸序列至少约96%、97%、98%或99%同源。
为了确定两种氨基酸序列(例如一种本发明的氨基酸序列和其突变体)或两种核酸的同源性百分比,进行最佳比较的序列对比(例如可以在一种蛋白质或核酸的序列引入空位,以与另一种蛋白质或核酸进行最佳序列对比)。然后比较对应氨基酸位置或核苷酸位置上氨基酸残基或核苷酸。当一个序列(如一种本发明的氨基酸序列)中的一个位置被另一个序列(例如所述氨基酸序列的突变体)中相应位置的同一氨基酸残基或核苷酸占据时,则所述两种分子在该位置是同源的(即本文所用的氨基酸或核酸“同源性”等同于氨基酸或核酸“同一性”)。这两个序列之间的同源性百分比是所述序列共享相同位置数的函数(即同源性百分比=相同位置数/位置总数×100)。
编码与本发明蛋白序列(例如序列表偶数SEQ ID NO序列)同源的SMP蛋白的分离的核酸分子可以通过以下步骤构建:在本发明的核苷酸序列中,导入一个或多个核苷酸取代、添加或缺失,使得在所编码的蛋白质中导入一个或多个氨基酸取代、添加或缺失。可以采用标准技术,例如定点诱变和PCR介导的突变,将突变导入一种本发明的核苷酸序列中。最好是在一个或多个预测的非必需氨基酸残基上进行保守氨基酸取代。“保守氨基酸取代”是其中所述氨基酸残基被具有相似侧链的氨基酸残基取代的氨基酸取代。在本领域中已经确定了具有相似侧链的氨基酸残基类别。这些类别包括具有碱性侧链的氨基酸(例如赖氨酸、精氨酸、组氨酸)、具有酸性侧链的氨基酸(例如天冬氨酸、谷氨酸)、具有不带电荷的极性侧链的氨基酸(例如甘氨酸、天冬酰胺、谷氨酰胺、丝氨酸、苏氨酸、酪氨酸、半胱氨酸)、具有非极性侧链的氨基酸(例如丙氨酸、缬氨酸、亮氨酸、异亮氨酸、脯氨酸、苯丙氨酸、甲硫氨酸、色氨酸)、具有β-分支侧链的氨基酸(例如苏氨酸、缬氨酸、异亮氨酸)和具有芳族侧链的氨基酸(例如酪氨酸、苯丙氨酸、色氨酸、组氨酸)。因此,SMP蛋白中预测的非必需氨基酸残基最好被来自同一侧链类别的另一种氨基酸残基取代。或者,在另一实施方案中,可以沿所有或部分SMP编码序列随机导入突变,例如通过饱和诱变导入突变,并且可以根据本文所述的SMP活性对所产生的突变体进行筛选,以鉴定保留SMP活性的突变体。对序列表奇数SEQ ID NO之一的核苷酸序列诱变后,可以重组表达所编码的蛋白质,然后可以采用例如本文所述的测定(参见实施例部分的实施例8)测定所述蛋白质的活性。
除了上述编码SMP蛋白的核酸分子外,本发明的另一方面涉及反义的分离的核酸分子。“反义”核酸包括与编码蛋白质的“有义”核酸互补的核苷酸序列,例如与双链DNA的编码链互补或与mRNA序列互补的核苷酸序列。因此,反义核酸可以与有义核酸形成氢键。所述反义核酸与完整的SMP编码链或仅与其部分互补。在一个实施方案中,反义核酸分子是编码SMP蛋白的核苷酸序列编码链的“编码区”的反义核酸分子。术语“编码区”是指包含被翻译为氨基酸残基的密码子的核苷酸序列区(例如SEQ ID NO:3(RXA01626)的完整编码区包含核苷酸1-345)。在另一实施方案中,所述反义核酸分子是编码SMP的核苷酸序列编码链“非编码区”的反义核酸分子。术语“非编码区”是指邻接所述编码区、不被翻译为氨基酸的5’序列和3’序列(即也称为5’和3’非翻译区)。
已知本文公开的编码SMP的编码链序列(例如序列表奇数SEQ IDNO中所示的序列),因此可以依照Waston和Crick碱基配对原则,设计本发明的反义核酸。所述反义核酸分子可以与SMP mRNA的完整编码区互补,但更优选是仅与SMP mRNA编码区或非编码区的一部分反义的寡核苷酸。例如,所述反义寡核苷酸可以与SMPmRNA翻译起始位点周围的区段互补。例如,所述反义寡核苷酸可以例如约5个、10个、15个、20个、25个、30个、35个、40个、45个或50个核苷酸长。可以采用化学合成以及采用本领域已知的方法的酶连接反应,构建本发明的反义核酸。例如,可以用天然存在的核苷酸或各种修饰的核苷酸化学合成反义核酸(如反义寡核苷酸),所述修饰的核苷酸设计用以增加所述分子生物学稳定性或增加所述反义核酸和有义核酸之间所形成的双链体物理稳定性,例如可以使用硫代磷酸衍生物以及吖啶取代的核苷酸。可以用来产生所述反义核酸的修饰的核苷酸的实例包括5-氟尿嘧啶、5-溴尿嘧啶、5-氯尿嘧啶、5-碘尿嘧啶、次黄嘌呤、黄嘌呤、4-乙酰胞嘧啶、5-(羧基羟甲基)尿嘧啶、5-羧甲基氨基甲基-2-硫代尿苷、5-羧甲基氨基甲基尿嘧啶、二氢尿嘧啶、β-D-半乳糖基Q核苷(queosine)、肌苷、N6-异戊烯基腺嘌呤、1-甲基鸟嘌呤、1-甲基肌苷、2,2-二甲基鸟嘌呤、2-甲基腺嘌呤、2-甲基鸟嘌呤、3-甲基胞嘧啶、5-甲基胞嘧啶、N6-腺嘌呤、7-甲基鸟嘌呤、5-甲基氨基甲基尿嘧啶、5-甲氧基氨基甲基-2-硫尿嘧啶、β-D-甘露糖基Q核苷、5’-甲氧基羧基甲基尿嘧啶、5-甲氧基尿嘧啶、2-甲硫基-N6-异戊烯基腺嘌呤、尿嘧啶-5-羟基乙酸(v)、wybutoxosine、假尿嘧啶、Q核苷、2-硫代胞嘧啶、5-甲基-2-硫尿嘧啶、2-硫尿嘧啶、4-硫尿嘧啶、5-甲基尿嘧啶、尿嘧啶-5-羟基乙酸甲酯、尿嘧啶-5-羟基乙酸(v)、5-甲基-2-硫尿嘧啶、3-(3-氨基-3-N-2-羧丙基)尿嘧啶、(acp3)w和2,6-二氨基嘌呤。另一方面,可以采用已经以反义方向亚克隆了一种核酸(即由所插入的核酸转录出的RNA将具有所感兴趣的靶核酸的反义方向,在以下小节中进一步描述)的表达载体,用生物学方法产生所述反义核酸。
通常将本发明的反义核酸分子给予细胞或使其原位产生,使得它们与编码SMP蛋白的细胞mRNA和/或基因组DNA杂交或结合,以由此抑制所述蛋白的表达,例如通过抑制转录和/或翻译而抑制表达。可以借助常规的核苷酸互补性进行杂交,以形成稳定的双链体,或例如在与DNA双链体结合的反义核酸分子的情况下,通过双螺旋主沟中的特异性相互作用而进行杂交。可以对所述反义分子进行修饰,使得它例如通过使所述反义核酸分子与结合于细胞表面受体或抗原的肽或抗体连接,而特异性地结合于所选定细胞表面表达的受体或抗原。可以用本文描述的载体将所述反义核酸分子传递至细胞。为了获得足够胞内浓度的所述反义分子,优选其中所述反义核酸分子置于强原核生物、病毒或真核生物启动子控制之下的载体构建物。
在再一实施方案中,本发明的反义核酸分子是α-异头核酸分子。α-异头核酸分子与互补RNA形成特定的双链杂交体,其中与通常的β单位相反,所述链互相平行(Gaultier等(1987)Nucleic Acids.Res.15:6625-6641)。所述反义核酸分子也可以包含2’-o-甲基核糖核苷酸(Inoue等(1987)Nucleic Acids Res.15:6131-6148)或嵌合RNA-DNA类似物(Inoue等(1987)FEBSLett.215:327-330)。
在又一实施方案中,本发明的反义核酸是一种核酶。核酶是具有核糖核酸酶的催化性RNA分子,它们能够切割与其具有互补区的单链核酸,例如mRNA。因此,可以用核酶(例如锤头核酶(描述于Haselhoff和Gerlach(1988)Nature 334:585-591)来催化切割SMP mRNA转录物,由此抑制SMP mRNA的翻译。可以根据本文公开的SMP cDNA的核苷酸序列(即SEQ ID NO.3(RXA01626),设计对SMP编码核酸具有特异性的核酶。例如,构建一种四膜虫属(Tetrahymena)L-19 IVSRNA的衍生物,其中活性部位的核苷酸序列与SMP编码mRNA中待切割的核苷酸序列互补。参见例如Cech等的美国专利号4,987,071和Cech等的美国专利号5,116,742。或者,可以用SMPmRNA,从RNA分子库中选择具有特定核糖核酸酶活性的催化性RNA。参见例如Bartel,D.和Szostak,J.W.(1993)Science 261:1411-1418。
另一方面,通过靶向与SMP核苷酸调节区(例如SMP启动子和/或增强子)互补的核苷酸序列,以形成阻止靶细胞中SMP基因转录的三螺旋结构,可以抑制SMP基因的表达。一般参见Helene,C.(1991)Anticancer Drug Des.6(6):569-84;Helene,C.等(1992)Ann.N.Y.Acad.Sci.660:27-36;和Maher,L.J.(1992)Bioassays 14(12):807-15。B.重组表达载体和宿主细胞
本发明的另一方面涉及含编码SMP蛋白(或其部分)的核酸的载体,优选表达载体。本文所用的术语“载体”是指能够转运与其连接的另一种核酸的核酸分子。一种类型的载体是“质粒”,质粒是指其中可以连接另外的DNA区段的环状双链DNA环。另一种类型的载体是病毒载体,其中可以将另外的DNA区段连接到病毒基因组中。某些载体能够在它们所导入的宿主细胞中自主复制(例如具有细菌复制起点的细菌载体和附加型哺乳动物载体)。其它载体(例如非附加型哺乳动物载体)在导入到宿主细胞后整合到宿主细胞的基因组中,因此与宿主基因组一起复制。此外,某些载体能够指导与其有效连接的基因的表达。这类载体在本文中称为“表达载体”,一般而言,在重组DNA技术中使用的表达载体通常是质粒形式的载体。在本说明书中,“质粒”和“载体”可以互换使用,因为质粒是最使用的载体形式。然而,本发明包括这类其它形式的表达载体,例如病毒载体(例如复制缺陷型反转录病毒、腺病毒和腺伴随病毒),它们发挥等同的功能。
本发明重组表达载体包含适合于在宿主细胞中表达本发明核酸的形式的本发明核酸,这是指所述重组表达载体包括根据用于表达的宿主细胞选择的一个或多个调节序列,所述调节序列有效连接于待表达的核酸序列。在重组表达载体中,“有效连接的”是指将所感兴趣的核苷酸序列以允许所述核苷酸序列表达的方式(例如在体外转录/翻译系统中或当将所述载体导入宿主细胞时在所述宿主细胞中)连接于所述调节序列。术语“调节序列”包括启动子、增强子和其它表达控制元件(例如聚腺苷酸化信号)。这类调节序列描述于例如Goeddel;Gene Expression Technology:Methods in Enzymology 185,AcademicPress,San Diego,CA(1990)。调节序列包括在许多类型宿主细胞中指导核苷酸序列组成型表达的调节序列、以及仅在某些宿主细胞中指导所述核苷酸序列表达的调节序列。优选的调节序列是例如启动子,例如cos-、tac-、trp-、tet-、trp-tet、lpp-、lac-、lpp-lac、lacIq、T7-、T5-、T3-、gal-、trc-、ara-、SP6-、arny、SPO2、λ-PR-或λPL,它们优选用于细菌中。其它调节序列是例如酵母和真菌的启动子,例如ADCl、MFα、AC、P-60、CYCl、GAPDH、TEF、rp28、ADH;植物启动子,例如CaMV/35S、SSU、OCS、lib4、usp、STLSl、B33、nos或遍在蛋白启动子或菜豆蛋白启动子。也可以使用人工启动子。本领域技术人员知道,所述表达载体的设计取决于诸如待转化的宿主细胞的选择、需要蛋白的表达水平等的因素。可以将本发明的表达载体导入宿主细胞中,由此产生由本文所述的核酸编码的蛋白质或肽,包括融合蛋白或肽(例如SMP蛋白、突变形式的SMP蛋白、融合蛋白等)。
可以设计本发明的重组表达载体用于在原核细胞或真核细胞中表达SMP蛋白。例如,可以在以下细胞中表达SMP基因:细菌细胞例如谷氨酸棒杆菌、昆虫细胞(使用杆状病毒表达载体)、酵母和其它真菌细胞(参见Romanos,M.A.等(1992)“酵母中的外源基因表达:综述”,Yeast 8:423-488;van den Hondel,C.A.M.J.J.等(1991)“在丝状真菌中的异源基因表达”,载于:More Gene Manipulations in Fungi,J.W.Bennet和L.L.Lasure编著,第396-428页:Academic Press:San Diego;和van den Hondel,C.A.M.J.J.和Punt,P.J.(1991),“用于丝状真菌的基因转移系统和载体的开发”,载于:Applied Molecular Genetics of Fungi,Peberdy,J.F.等编著,第1-28页,Cambridge University Press:Cambridge)、藻类和多细胞植物细胞(参见Schmidt,R.和Willmitzer,L.(1988)“高效根癌农杆菌(Agrobacterium tumefaciens)介导的拟南芥(Arabidopsis thaliana)和子叶外植体的转化”Plant Cell Rep.:583-586)或哺乳动物细胞。合适的宿主细胞在Goeddel,Gene ExpressionTechnology:Methods in Enzymology 185,Academic Press,San Diego,CA(1990)中有进一步的描述。或者,所述重组表达载体可以在体外例如用T7启动子调节序列和T7聚合酶进行转录和翻译。
在原核生物中表达蛋白最常用含有指导融合蛋白或者非融合蛋白表达的组成型或诱导型启动子的载体来进行。融合载体将多个氨基酸加至其中所编码蛋白上,通常加至所述重组蛋白的氨基末端,但也加至C末端,或在所述蛋白质中的合适区中融合。这类融合载体通常具有三个作用:1)增加重组蛋白的表达;2)增加所述重组蛋白的溶解性;和3)通过用作亲和纯化中的配体而有助于所述重组蛋白的纯化。通常,在融合表达载体中,在融合部分和重组蛋白的接点处引入一个蛋白酶切割位点,以使得能够在纯化所述融合蛋白后将重组蛋白与融合部分开。这类酶及其相关的识别序列包括因子Xa、凝血酶和肠激酶。
典型的融合表达载体包括pGEX(Pharmacia Biotech Inc;Smith,D.B.和Johnson,K.S.(1988)Gene 67:31-40)、pMAL(New EnglandBiolabs,Beverly,MA)和pRIT5(Pharmacia,Piscataway,NJ),它们分别将谷胱甘肽S-转移酶(GST)、麦芽糖E结合蛋白或A蛋白与靶重组蛋白融合。在一个实施方案中,将所述SMP蛋白的编码序列克隆到pGEX表达载体中,以产生编码融合蛋白的载体,所述融合蛋白从N末端至C末端包含GST-凝血酶切割位点-X蛋白。所述融合蛋白可以用谷胱甘肽-琼脂糖树脂通过亲和层析纯化。不与GST融合的重组SMP蛋白可以通过用凝血酶切割所述融合蛋白进行回收。
合适的诱导型非融合大肠杆菌表达载体的实例包括pTrc(Amann等,(1988)Gene 69:301-315)、pLG338、pACYC184、pBR322、pUC18、pUC19,pKC30、pRep4、pHS1、pHS2、pPLc236、pMBL24、pLG200、pUR290、pIN-III113-B1、λgt11、pBdCl和pET11d(Studier等,GeneExpression Technology: Methos in Enzymology 185,Academic Press,SanDiego,California(1990)60-89;和Pouwels等编著(1985)Cloning Vectors.Elsevier:纽约IBSN 0 444 904018)。pTrc载体靶基因的表达依赖于杂种trp-lac融合启动子的宿主RNA聚合酶转录。pET11d载体的靶基因的表达依赖于通过共同表达的病毒RNA聚合酶(T7 gn1)T7 gn10-lac融合启动子的转录。这种病毒聚合酶由含有lacUV5启动子转录控制下的T7 gn1基因的居留(resident)λ原噬菌体的宿主菌株BL21(DE3)或HMS174(DE3)供应。关于其它细菌变种的转化,可以选择合适的载体。例如,已知质粒pIJ101、pIJ364、pIJ702和pIJ361可用于转化链霉菌属(Streptomyces),质粒pUB110、pC194或pBD214适用于转化芽孢杆菌属(Bacillus)菌种。用于将遗传信息转移到棒杆菌属的几种质粒包括pHM1519、pBL1、pSA77或pAJ667(Pouwels等编著(1985)CloningVectors.Elsevier:纽约IBSN 0444 904018)。
使重组蛋白表达最大化的一种策略是在蛋白酶剪切所述重组蛋白能力受损的宿主细菌中表达所述蛋白(Gottesman,S.,GeneExpression Technology: Methods in Enzymology 185,Academic Press,SanDiego,California(1990)119-128)。另一种策略是改变待插入到表达载体中的核酸的核酸序列,使得每个氨基酸的各个密码子是在选定用于表达的细菌(例如谷氨酸棒杆菌)中优先使用的密码子(Wada等(1992)Nucleic Acids Res.20:2111-2118)。这种本发明核酸序列的改变可以用标准DNA合成技术进行。
在另一实施方案中,所述SMP蛋白表达载体是一种酵母表达载体。用于在酵母酿酒酵母(S.cerevisiae)中表达的载体的实例包括pYepSecl(Baldari等(1987)Embo J.6:229-234)、2μ、pAG-1、Yep6、Yep13、pEMBLYe23、pMFa(Kurjan和Herskowitz(1982)Cell 30:933-943)、pJRY88(Schultz等,(1987)Gene 54:113-123)和pYES2(InvitrogenCorporation,San Diego,CA)。适用于其它真菌例如丝状真菌的载体以及载体构建方法包括在以下文献中详述的载体和方法:van den Hondel,C.A.M.J.J.和Punt,P.J.(1991)“用于丝状真菌的基因转移系统和载体的开发”,载于:Applied Molecular Genetics of Fungi,J.F.Peberdy等编著,第1-28页,Cambridge University Press:Cambridge,和Pouwels等编著(1985)Cloning Vectors.Elsevier:纽约(IBSN 0444 904018)。
另一方面,本发明的SMP蛋白可以用杆状病毒表达载体在昆虫细胞中表达。可用于在培养的昆虫细胞(例如Sf9细胞)中表达蛋白的杆状病毒载体包括pAc系列(Smith等(1983)Mol.Cell Biol.3:2156-2165)和pVL系列(Lucklow和Summers(1989)Virology 170:31-39)。
在另一实施方案中,本发明的SMP蛋白可以在单细胞植物细胞(例如藻类)或在高等植物(例如种子植物,诸如作物)的植物细胞中表达。植物表达载体的实例包括详述于以下文献中的表达载体:Becker,D.,Kemper,E.,Schell,J.和Masterson,R.(1992)“在左边界附近具有选择标记的新型植物双元载体”,Plant Mol.Biol.20:1195-1197;和Bevan,M.W.(1984)“用于植物转化的双元农杆菌载体”,Nucl.Acid.Res.12:8711-8721,并且包括pLGV23、pGHlac+、pBIN19、pAK2004和pDH51(Pouwels等编著(1985)Cloning Vectors.Elsevier:纽约IBSN 0444904018)。
在再一实施方案中,本发明的核酸用哺乳动物表达载体在哺乳动物细胞中表达。哺乳动物表达载体的实例包括pCDM8(Seed,B.(1987)Nature 329:840)和pMT2PC(Kaufman等(1987)EMBO J.6:187-195)。当用于哺乳动物细胞中时,所述表达载体的控制功能通常由病毒调节元件提供。例如,常用的启动子得自多瘤病毒、腺病毒2、巨细胞病毒和猿猴病毒40。对于用于原核细胞和真核细胞的其它合适的表达系统,参见Sambrook,J.,Fritsh,E.F.和Maniatis,T.Molecular Cloning:ALaboratory Manual.第2版的第16和17章,Cold Spring HarborLaboratory,Cold Spring Harbor Laboratory Press,Cold Spring Harbor,NY,1989。
在另一实施方案中,重组哺乳动物表达载体能够指导所述核酸优先在特定的细胞类型中表达(例如用组织特异性调节元件来表达所述核酸)。组织特异性调节元件是本领域已知的。合适的组织特异性启动子的非限制性实例包括清蛋白启动子(肝特异性;Pinkert等(1987)Genes Dev.1:268-277)、淋巴(lymphoid)特异性启动子(Calame和Eaton(1988)Adv.Immunol.43:235-275)、特别是T细胞受体的启动子(Winoto和Baltimore(1989)EMBO J.8:729-733)和免疫球蛋白启动子(Banerji等(1983)Cell 33:729-740;Queen和Baltimore(1983)Cell 33:741-748)、神经元特异性启动子(例如神经丝启动子;Byrne和Ruddle(1989)PNAS86:5473-5477)、胰腺特异性启动子(Edlund等(1985)Science 230:912-916)和乳腺特异性启动子(例如乳清启动子;美国专利号4,873,316和欧洲专利申请号264,166)。也包括发育调节型启动子,例如鼠类hox启动子(Kessel和Gruss(1990)Science 249:374-379)和甲胎蛋白启动子(Campes和Tilghman(1989)Genes Dev.3:537-546)。
本发明还提供包含以反义方向克隆到表达载体中的本发明DNA分子的重组表达载体。亦即所述DNA分子与调节序列有效地连接,其连接方式提供SMP mRNA的反义RNA分子的表达(通过转录所述DNA分子)。可以选择有效连接于反义方向克隆的核酸、指导在多种细胞类型中连续表达所述反义RNA分子的调节序列,例如病毒启动子和/或增强子,或者可以选择指导组成型、组织特异性或细胞类型特异性表达反义RNA的调节序列。反义表达载体可以为重组载体、噬菌粒或减毒病毒的形式,其中反义核酸在高效调节区的控制之下产生,其活性可以由所述载体所导入的细胞类型决定。对于使用反义基因的基因表达调节的讨论,参见Weintraub,H.等,作为遗传分析分子工具的反义RNA,Reviews-Trends in Genetics,第1(1)卷1986。
本发明的另一方面涉及已经导入本发明重组表达载体的宿主细胞。术语“宿主细胞”和“重组宿主细胞”在本文中可互换使用。不言而喻,这种术语不仅是指特定的题述细胞,而且也指这种细胞的子代或潜在的子代。因为在连续世代中由于突变或者环境影响而可能发生某些修饰,所以这类子代事实上可能与亲代细胞不完全相同,但仍包括在本文所述的术语范围内。
宿主细胞可以是任何原核细胞或真核细胞。例如,SMP蛋白可以在细菌细胞例如谷氨酸棒杆菌、昆虫细胞、酵母细胞或哺乳动物细胞(例如中国仓鼠卵巢细胞(CHO)或COS细胞)中表达。其它合适的宿主细胞是本领域技术人员已知的。与谷氨酸棒杆菌相关的、可以方便地用作本发明核酸和蛋白分子的宿主细胞的微生物示于表3。
可以通过常规转化或转染技术,将载体DNA导入原核细胞或真核细胞中。本文所用的术语“转化”和“转染”、“接合”和“转导”是指本领域公知的用于将外源核酸(如线性DNA或RNA(如线性化载体或不包括载体的基因构建物)或载体形式的核酸(例如质粒、噬菌体、尾感器(phasmid)、噬菌粒(phagemid)、转座子或其它DNA))导入宿主细胞的多种技术,包括磷酸钙或氯化钙共沉淀、DEAE-葡聚糖介导的转染、脂转染、天然感受态、化学物质介导的转移或电穿孔。用于转化或转染宿主细胞的合适方法可以在Sambrook等(MolecularCloning:A Laboratory Manual.第2版,Cold Spring Harbor Laboratory,Cold Spring Harbor Laboratory Press,Cold Spring Harbor,NY,1989)和其它实验手册中找到。
关于哺乳动物细胞的稳定转染,已知根据所用的表达载体和转染技术,仅一小部分细胞可以将外源DNA整合到其基因组中。为了鉴定和选择这些整合子,通常将编码选择标记(如抗生素抗性)的基因与目的基因一起导入宿主细胞中。优选的选择标记包括赋予对药物例如G418、潮霉素和氨甲蝶呤的抗性的选择标记。可以将编码选择标记的核酸在编码SMP蛋白的同一载体上导入宿主细胞,或者可以将其在单独的载体上导入。可以通过药物选择鉴定所导入核酸稳定转染的细胞(例如已经掺入选择标记基因的细胞将存活,而其它细胞死亡)。
为了获得同源重组微生物,制备含有至少一部分SMP基因的载体,在所述SMP基因部分中已经引入缺失、添加或取代以由此改变(例如功能性破坏)所述SMP基因。最好该SMP基因是谷氨酸棒杆菌SMP基因,但它可以是来自相关细菌或甚至哺乳动物、酵母或昆虫来源的同系物。在一个优选实施方案中,设计所述载体,使得在同源重组后,所述内源SMP基因被功能性破坏(即不再编码功能性蛋白;也称为“敲除”载体)。或者,可以设计所述载体,使得在同源重组后,所述内源SMP基因被突变或者被改变,但仍编码功能性蛋白(例如可以改变上游调节区,以由此改变所述内源SMP蛋白的表达)。在所述同源重组载体中,所述SMP基因的改变部分在其5’端和3’端邻接另一种SMP基因的核酸,使得能够在所述载体所携带的外源SMP基因和微生物中的内源SMP基因之间发生同源重组。所述另一种侧翼SMP核酸具有足以与内源基因成功同源重组的长度。通常,在所述载体中包含数千碱基的侧翼DNA(5’端以及3’端)(有关同源重组载体的描述,参见例如Thomas,K.R.和Capecchi,M.R.(1987)Cell 51:503)。将所述载体导入微生物中(例如通过电穿孔),然后用本领域已知的技术选择其中所导入的SMP基因与内源SMP基因同源重组的细胞。
在另一实施方案中,可以产生含有选定系统的重组微生物,所述选定系统提供受调节的所导入基因的表达。例如,在载体上包含在置于lac操纵子控制之下的SMP基因,允许所述SMP基因仅在IPTG存在时表达。这类调节系统是本领域众所周知的。
在另一实施方案中,破坏宿主细胞中的内源SMP基因(例如通过同源重组或本领域已知的其它遗传方法),使得不发生其蛋白产物的表达。在另一实施方案中,通过一个或多个点突变、缺失或倒位,已经改变了宿主细胞中内源的或所导入的SMP基因,但它仍编码功能性SMP蛋白。在再一实施方案中,改变了微生物中SMP基因的一个或多个调节区(例如启动子、阻抑蛋白或诱导物)(例如通过缺失、截短、倒位或点突变),使得调节所述SMP基因的表达。本领域技术人员知道,含有一种以上的所述SMP基因和蛋白修饰的宿主细胞可以采用本发明的方法容易地产生,并且包括在本发明内。
本发明的宿主细胞(例如培养物中的原核细胞或真核细胞)可以用来产生(即表达)SMP蛋白。因此,本发明还提供使用本发明的宿主细胞生产SMP蛋白的方法。在一个实施方案中,所述方法包括在合适的培养基中培养本发明的宿主细胞(其中已经导入了编码SMP蛋白的重组表达载体,或在其基因组中导入了编码野生型或改变的SMP蛋白的基因),直至产生SMP蛋白。在另一实施方案中,所述方法还包括从培养基中或从宿主细胞中分离SMP蛋白。C.分离的SMP蛋白
本发明的另一方面涉及分离的SMP蛋白及其生物活性部分。“分离的”或“纯化的”蛋白或其生物活性部分当通过重组DNA技术产生时基本上不含细胞物质,或在化学合成时基本上不含化学前体或其它化学物质。用语“基本上不含细胞物质”包括其中SMP蛋白质与细胞中天然产生或重组产生的细胞组分分离的SMP蛋白制备物。在一个实施方案中,用语“基本上不含细胞物质”包括所含有的非SMP蛋白(本文中也称为“污染蛋白”)低于约30%(以干重计)、更优选低于20%、再更优选低于约10%、最优选低于约5%的SMP蛋白制备物。当重组产生所述SMP蛋白或其生物活性部分时,其最好也基本不含培养基。即培养基少于约20%,更优选少于约10%,最优选少于约5%的蛋白制备物量。用语“基本上不含化学前体或其它化学物质”包括其中SMP蛋白与涉及所述蛋白合成的化学前体或其它化学物质分离的SMP蛋白制备物。在一个实施方案中,术语“基本上不含化学前体或其它化学物质”包括所具有的化学前体或非SMP化学物质低于约30%(以干重计)、更优选低于20%、再更优选低于约10%、最优选低于约5%的SMP蛋白制备物。在优选实施方案中,分离的蛋白质或其生物活性部分没有来自获得所述SMP蛋白的同一生物的污染蛋白。通常,这类蛋白通过在微生物例如谷氨酸棒杆菌中表达例如谷氨酸棒杆菌SMP蛋白来产生。
本发明的分离的SMP蛋白或其部分可参与谷氨酸棒杆菌诸如糖的碳化合物代谢,或产生用于驱动不利代谢途径的能量化合物(例如通过氧化磷酸化),或具有表1所述的一种或多种活性。在优选实施方案中,所述蛋白质或其部分包含与本发明氨基酸序列(例如序列表偶数SEQ ID NO序列)足够同源的氨基酸序列,使得所述蛋白质或其部分保留执行参与谷氨酸棒杆菌诸如糖的碳化合物代谢和通过诸如氧化磷酸化的过程产生能量分子的能力。所述蛋白的所述部分最好是本文所述的生物活性部分。在另一优选实施方案中,本发明的SMP蛋白具有序列表中一个偶数SEQ ID NO中所示的氨基酸序列。在再一优选实施方案中,所述SMP蛋白具有在严格条件下与本发明核苷酸序列(例如序列表奇数SEQ ID NO序列)杂交的核苷酸序列所编码的氨基酸序列。在又一优选实施方案中,所述SMP蛋白具有由下述的核苷酸序列编码的氨基酸序列或其部分,其中所述核苷酸序列与一种本发明核酸序列的同源性为至少约50%、51%、52%、53%、54%、55%、56%、57%、58%、59%或60%,优选至少约61%、62%、63%、64%、65%、66%、67%、68%、69%或70%,更优选至少约71%、72%、73%、74%、75%、76%、77%、78%、79%或80%、81%、82%、83%、84%、85%、86%、87%、88%、89%或90%、或91%、92%、93%、94%,甚至更优选至少约95%、96%、97%、98%、99%或更高。本发明也包括上述数值之间的范围和标识数值(例如70-90%相同或80-95%相同)。例如,包括采用任何组合的上述值作为上限和/或下限的标识值范围。本发明优选的SMP蛋白也最好具有本文所述的至少一种SMP活性。例如,本发明的优选SMP蛋白包括由与一种本发明核酸序列杂交、例如在严格条件下杂交的核苷酸序列编码的氨基酸序列,并且可以参与谷氨酸棒杆菌诸如糖的碳化合物代谢和通过诸如氧化磷酸化的过程产生能量分子(例如ATP),或者具有表1给出的一种或多种活性。
在其它实施方案中,所述SMP蛋白与一种本发明的氨基酸序列(例如序列表偶数SEQ ID NO序列)基本上同源,并且保留本发明氨基酸序列之一的蛋白的功能活性,但由于天然变异或诱变在氨基酸序列有所不同,如以上I小节中详细描述的。因此,在另一实施方案中,所述SMP蛋白是这样一种蛋白质:所述蛋白质所包含的氨基酸序列与一种本发明的完整氨基酸序列的同源性为至少约50%、51%、52%、53%、54%、55%、56%、57%、58%、59%或60%,优选至少约61%、62%、63%、64%、65%、66%、67%、68%、69%或70%,更优选至少约71%、72%、73%、74%、75%、76%、77%、78%、79%或80%、81%、82%、83%、84%、85%、86%、87%、88%、89%或90%、或91%、92%、93%、94%,甚至更优选至少约95%、96%、97%、98%、99%或更高,并且具有本文所述的至少一种SMP活性。本发明也包括上述数值之间的范围和标识数值(例如70-90%相同或80-95%相同)。例如,包括采用任何组合的上述值作为上限和/或下限的标识值范围。在另一实施方案中,本发明涉及与一种本发明的完整氨基酸序列基本上同源的全长的谷氨酸棒杆菌蛋白。
SMP蛋白的生物活性部分包括包含衍生自SMP蛋白氨基酸序列(例如序列表偶数SEQ ID NO:的氨基酸序列)的氨基酸序列、或与SMP蛋白同源的蛋白氨基酸序列的肽,其包含的氨基酸少于全长SMP蛋白或与SMP蛋白同源的全长蛋白质并且具有至少一种SMP蛋白活性。通常,生物活性部分(肽,例如长度例如为5个、10个、15个、20个、30个、35个、36个、37个、38个、39个、40个、50个、100个或更多个氨基酸的肽)包含具有至少一种SMP蛋白的活性的结构域或基序。此外,其中所述蛋白质的其它区缺失的其它生物活性部分可以通过重组技术制备,并且根据本文所述的一种或多种活性进行评价。最好是SMP蛋白的生物活性部分包含具有生物活性的一个或多个选定结构域/基序或其部分。
SMP蛋白最好通过重组DNA技术产生。例如,将编码所述蛋白质的核酸分子克隆到表达载体(如上所述)中,将所述表达载体导入宿主(如上所述),然后在所述宿主细胞中表达所述SMP蛋白。然后可以通过合适的纯化方案,采用标准蛋白质纯化技术,从所述细胞中分离出所述SMP蛋白。作为重组表达的替代方法,可以用标准肽合成技术,化学合成SMP蛋白、多肽或肽。此外,可以例如采用通过标准技术、利用本发明的SMP蛋白或其片段产生的抗SMP抗体,从所述细胞(例如内皮细胞)中分离天然SMP蛋白。
本发明也提供SMP嵌合蛋白或融合蛋白。本文所用的SMP“嵌合蛋白”或“融合蛋白”包含与非SMP多肽有效连接的SMP多肽。“SMP多肽”是指具有相当于SMP蛋白的氨基酸序列的多肽,而“非SMP多肽”是指所具有的氨基酸序列相当于与所述SMP蛋白基本上不同源的蛋白质的多肽,例如不同于所述SMP蛋白并且得自同一生物或不同生物的蛋白质。在所述融合蛋白中,术语“有效连接的”是指所述SMP多肽和所述非SMP多肽彼此按读框融合。所述非SMP多肽可以融合至所述SMP多肽的N末端或C末端。例如,在一种实施方案中,所述融合蛋白是其中SMP序列融合至GST序列的C末端的GST-SMP融合蛋白。这类融合蛋白可以便于纯化重组SMP蛋白。在另一实施方案中,所述融合蛋白是在N末端含有一个异源信号序列的SMP蛋白。在某些宿主细胞(例如哺乳动物宿主细胞中),可以通过利用异源信号序列增加SMP蛋白的表达和/或分泌。
最好是本发明的SMP嵌合蛋白或融合蛋白采用标准重组DNA技术来生产。例如,依照常规技术,例如通过利用平端或交错切口末端进行连接、进行限制性酶消化以提供合适的末端、合适的时候补平粘端、进行碱性磷酸酶处理以避免不希望有的连接,然后进行酶连接,将编码不同多肽序列的DNA片段符合读框地连接在一起。在另一实施方案中,通过常规技术,包括自动DNA合成仪,可以合成所述融合基因。或者,采用锚定引物进行基因片段的PCR扩增,所述锚定引物在两个连续基因片段之间产生互补突出端,随后将其退火并重新扩增,产生嵌合基因序列(参见例如Current Protocols in Molecular Biology,Ausubel等编著,John Wiley & Sons:1992)。此外,许多表达载体是市售的,已经编码一个融合部分(例如GST多肽)。可以将SMP编码核酸克隆到这样一种表达载体中,使得所述融合部分与所述SMP蛋白符合读框地融合。
通过对所述SMP蛋白进行诱变,例如离散的点突变或截短,可以产生所述SMP蛋白的同系物。本文所用的术语“同系物”是指用作所述SMP蛋白活性的激动剂或拮抗剂的所述SMP蛋白的变异形式。SMP蛋白的激动剂可以基本上保留所述SMP蛋白的相同生物活性或一个亚类的生物活性。SMP蛋白的拮抗剂通过例如竞争性结合包括SMP蛋白的糖分子代谢级联或能量产生途径的下游或上游成员,可以抑制天然形式SMP蛋白的一种或多种活性。
在一个替代实施方案中,通过筛选具有SMP蛋白激动剂或拮抗剂活性的所述SMP蛋白的突变体例如截短突变体的组合文库,可以鉴定出所述SMP蛋白的同系物。在一个实施方案中,通过在核酸水平上进行联合诱变,产生SMP变异体的一个花斑文库,所述花斑文库由一个花斑基因文库编码。通过例如酶法将合成的寡核苷酸混合物连接到基因序列中,使得一组简并的潜在SMP序列可作用单个多肽表达,或者作为其中含有一组SMP序列的一组较大的融合蛋白表达(例如用于噬菌体展示),可以获得SMP变异体花斑文库。有多种方法可以用来由简并的寡核苷酸序列产生潜在的SMP同系物文库。可以在自动DNA合成仪中进行简并基因序列的化学合成,然后将所述合成基因连接到合适的表达载体中。应用一组简并基因,使得可以在一种混合物中提供所有编码一组所需潜在SMP序列的序列。合成简并寡核苷酸的方法是本领域已知的(参见例如Narang,S.A.(1983)Tetrahedron 39:3;Itakura等(1984)Annu.Rev.Biochem.53:323;Itakura等(1984)Science 198:1056;Ike等(1983)Nucleic Acid Res.11:477)。
另外,所述SMP蛋白编码的片段的文库可以用来产生SMP片段的花斑群体,用来筛选和随后选择SMP蛋白的同系物。在一个实施方案中,如下产生编码序列片段的文库:在每个分子大约仅发生一个缺口的条件下用核酸酶处理SMP编码序列的双链PCR片段,使所述双链DNA变性,使所述DNA复性形成可以包括不同缺口产物的有义/反义对的双链DNA,通过用S1核酸酶处理从重新形成的双链体中去除单链部分,然后将所产生的片段文库连接到表达载体中。用这种方法,可以得到一个表达文库,所述表达文库编码各种大小的所述SMP蛋白的N末端片段、C末端片段和内部片段。
本领域已知几种技术用于筛选通过点突变或截短制备的组合文库的基因产物、以及筛选cDNA文库寻找具有选定特性的基因产物。这类技术适用于快速筛选通过组合诱变SMP同系物产生的基因文库。适合于高通量分析、用于筛选大基因文库的最为广泛使用的技术,包括将所述基因文库克隆到复制型表达载体中,用所产生的载体文库转化合适的细胞,并且使所述组合基因在所需活性的检测有助于载体分离的条件下表达,而所述载体编码其基因产物被检测的基因。循环总体诱变(REM)是一种提高文库中功能型突变体频率的新技术,可以与鉴定SMP同系物的筛选测定结合使用(Arkin和Yourvan(1992)PNAS89:7811-7815;Delgrave等(1993)Protein Engineering 6(3):327-331)。
在另一实施方案中,可以采用本领域中众所周知的方法,用基于细胞的测定来分析花斑SMP文库。D.本发明的应用和方法
本文所述的核酸分子、蛋白质、蛋白质同系物、融合蛋白、引物、载体和宿主细胞可以用于一种或多种以下方法中:鉴定谷氨酸棒杆菌和相关生物;与谷氨酸棒杆菌相关的生物的基因组作图;鉴定和定位目的谷氨酸棒杆菌序列;进化研究;测定功能所需的SMP蛋白区;调节SMP蛋白活性;调节一种或多种糖代谢;调节细胞产生高能分子(即ATP、NADPH);以及调节细胞产生需要化合物(例如精细化学品)。
本发明的SMP核酸分子具有多种用途。首先,它们可以用来鉴定谷氨酸棒杆菌和其密切相关的菌种。此外,它们可以用来鉴定微生物混合群体中是否谷氨酸棒杆菌和其相关菌种。本发明提供许多谷氨酸棒杆菌基因的核酸序列;通过在严格条件下用跨越谷氨酸棒杆菌特有基因区域的探针,探测一种微生物群体和混合微生物群体培养物所提取的基因组DNA,从而人们可以确定是否存在谷氨酸杆菌。
虽然谷氨酸棒杆菌自身是非致病性的,但它与致病菌种例如白喉棒杆菌相关。白喉棒杆菌是白喉病原体,白喉是一种涉及局部和系统病理学的快速发展的急性发热性感染。在这种疾病中,局部病变发生于上呼吸道,而且涉及上皮细胞坏死性损伤;白喉棒杆菌分泌毒素,毒素从局部病变弥散至机体的远处易感组织。因为抑制各种组织的蛋白质合成而发生变性,导致白喉系统病理学,所述组织包括心脏、肌肉、外周神经、肾上腺、肾脏、肝脏和脾。白喉在世界许多地区包括非洲、亚洲、欧洲东部和前苏联许多独立州中发病率一直很高。自从1990年以来,在后两个地区的一次白喉流行导致5,000人死亡。
在一个实施方案中,本发明提供一种鉴定受试者体内是否存在白喉棒杆菌或其活动情况的方法。这种方法包括鉴定受试者体内一种或多种本发明核酸序列或氨基酸序列(例如分别为序列表奇数或偶数SEQ ID NO中所示的序列),由此检测所述受试者体内是否存在谷氨酸棒杆菌或活动情况。谷氨酸棒杆菌和白喉棒杆菌是相关细菌,谷氨酸棒杆菌中的许多核酸和蛋白质分子与白喉棒杆菌的核酸和蛋白质分子同源,因此可以用来检测受试者体内的白喉棒杆菌。
本发明的核酸分子和蛋白质分子也可以用作基因组特定区的标记。这不仅可应用于基因组作图中,也可应用于谷氨酸棒杆菌蛋白的功能研究。例如,为了鉴定基因组中特定谷氨酸棒杆菌DNA结合蛋白所结合的区域,可以将谷氨酸棒杆菌基因组消化,然后将片段与所述DNA结合蛋白一起温育。结合所述蛋白的片段可以另外用本发明的核酸分子来探测,最好是用可容易检测的标记来探测;这种核酸分子与所述基因组片段的结合使得能够将所上述片段定位至谷氨酸棒杆菌的基因组图谱上,并且当用不同酶进行多次时,有助于所述蛋白所结合的核酸序列的快速测定。此外,本发明的核酸分子可能与相关菌种的序列具有足够的同源性,因此这些核酸分子可以用作在相关细菌例如乳发酵短杆菌中构建基因组图谱的标记。
本发明的SMP核酸分子也可用于进化研究和蛋白质结构研究。本发明分子参与的代谢和能量释放过程为各种各样的原核生物和真核生物细胞所利用;通过比较本发明核酸分子序列与编码其它生物的相似酶的核酸分子序列,可以评价生物的进化相关性。同样,这种比较允许评价所述序列的哪些区保守的、哪些区不保守,这有助于确定酶功能所必需的蛋白区。这种类型的测定对于蛋白质工程研究是有价值的,并且可以给出蛋白质在诱变方面可以耐受而不丧失功能的指标。
对本发明的SMP核酸分子的操作可以导致产生功能上不同于野生型SMP蛋白的SMP蛋白。这些蛋白质可能效率方面或活性方面得到改进,在细胞中的存在数量可能大于通常的数量,或可能效率或活性降低。
本发明提供筛选方法,用于筛选通过与蛋白质自身或所述SMP蛋白的底物或者结合配偶体相互作用、或者通过调节本发明SMP核酸分子的转录或翻译而调节SMP蛋白活性的分子。在这类方法中,使表达一种或多种本发明SMP蛋白的微生物与一种或多种试验化合物接触,并且评价每种试验化合物对所述SMP蛋白活性或表达水平的影响。
本发明SMP蛋白改变可以通过多种机制直接影响掺入这种改变蛋白的谷氨酸棒杆菌菌株产生精细化学品的产率、产量和/和生产效率。诸如糖的高能碳分子降解以及诸如NADH和FADH2的化合物通过氧化磷酸化转化为更有用的形式可以产生多种化合物,这些化合物本身可能就是需要的精细化学品,如丙酮酸、ATP、NADH和多种中间体糖化合物。进而,细胞可利用这些代谢途径产生的能量分子(如ATP)和还原当量物(如NADH或NADPH)驱动在能量上不利的反应。这样的不利反应包括许多精细化学品生物合成途径。人们通过改进细胞利用特定糖的能力(例如操作编码参与所述细胞降解转化糖为能量的酶的基因),可提高能量,以便细胞可进行不利但是仍然需要的代谢反应(例如生物合成需要精细化学品)。
此外,对参与糖利用的一个或多个途径的进行调节可优化转化糖分子含有的能量,以便产生一种或多种需要的精细化学品。例如通过降低参与例如葡糖异生的酶活性,可以获得更多供细胞驱动所需生化反应(例如精细化学品生物合成)的ATP。此外,也可以调节糖的总体能量分子产生以保证细胞由每个糖分子产生的能量最大化。无效的糖利用可能产生过量的二氧化碳和过量的能量,过量的能量可能产生无用的代谢循环。通过改进糖分子代谢,细胞应能够更有效地行使功能,而需要较少的碳分子。这也应可以获得改进的精细化学品:糖分子比(提高碳产率),而且可以减少百大规模发酵培养这种工程谷氨酸棒杆菌中必须加入培养基中的糖量。
诱变一种或多种本发明SMP基因也可能产生活性改变的SMP蛋白,活性改变的SMP蛋白间接影响谷氨酸棒杆菌产生一种或多种需要精细化学品。例如,人们通过提高谷氨酸棒杆菌利用一种或多种糖的效率(从而增强所述糖转化为有用的能量分子)、或者提高还原当量物转化为有用的能量分子的效率(例如通过提高氧化磷酸化效率或增强ATP合酶活性),能够增加这些高能化合物量,供细胞驱动通常不利的代谢过程。这样的不利过程包括构建细胞壁、转录、翻译以及生物合成细胞生长分裂必需的化合物(例如核苷酸、氨基酸、维生素、脂质等)(Lengeler等(1999)Biology of Prokaryotes,Thieme Verlag:Stuttgart,第88-109、913-918、875-899页)。通过改进这些工程细胞的生长和繁殖,既可提高大规模培养物中的细胞存活力,也可以提高其分裂率,这样更大量的细胞能够在发酵罐培养物中存活。至少因为存在更多的产生需要精细化学品的活细胞而提高产率、产量或生产效率。
此外,细胞本身利用糖代谢产生的许多降解产物作为产生无数其它有用化合物(其中部分是精细化学品)的前体或中间体。例如丙酮酸转化为氨基酸丙氨酸,而核糖-5-磷酸为例如核苷酸分子的一个组成部分。因此,糖代谢的量和效率对这些降解产物在细胞中的有效性有显著影响。通过在现有途径的效率方面增强细胞代谢糖的能力(例如工程改进参与这些途径的酶,从而优化其活性),或者通过提高参与这些途径的酶的有效性(例如增加细胞中存在的这些酶的数量),也可以提高这些降解产物在细胞中的有效性,从而再增强细胞产生许多不同的其它需要化合物(例如精细化学品)。
使谷氨酸棒杆菌精细化学品产率提高的上述SMP蛋白诱变策略不是限制性的;对这些策略进行改变对于本领域技术人员是显而易见的。采用这些策略并且加入本文公开的机制,可以利用本发明的核酸分子和蛋白质分子来产生表达突变型SMP核酸分子和蛋白质分子的谷氨酸棒杆菌或相关细菌菌种,使得需要化合物的产率、产量和/或生产效率得以改善。这种需要化合物可以是谷氨酸棒杆菌的任何产物,包括生物合成途径的终产物和天然存在的代谢途径的中间体,以及在谷氨酸棒杆菌代谢中不是天然存在的、但由本发明的谷氨酸棒杆菌菌株生产的分子。
应用以下实施例进一步阐明本发明,不应将其解释为限制本发明。在本申请中引用的所有参考文献、专利申请、专利、公布的专利申请、表格和序列表的内容均通过引用结合到本文中。
表1:本申请中的基因HMP:核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO1 2 RXS02735 VV0074 14576 15280 6-磷酸葡萄糖酸酶内酯酶3 4 RXA01626 GR00452 4270 3926 L-核酮糖-磷酸4-差向异构酶5 6 RXA02245 GR00654 13639 14295 核酮糖-磷酸3-差向异构酶(EC 5.1.3.1)7 8 RXA01015 GR00290 346 5 核糖5-磷酸异构酶(EC 5.3.1.6)TCA:核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO9 10 RXN01312 VV0082 20803 18785 琥珀酸脱氢酶黄素蛋白亚单位(EC 1.3.99.1)11 12 FRXA01312 GR00380 2690 1614 琥珀酸脱氢酶黄素蛋白亚单位(EC 1.3.99.1)13 14 RXN00231 VV0083 15484 14015 琥珀酸-半缩醛脱氢酶(NADP+)(EC 1.2.1.16)15 16 RXA01311 GR00380 1611 865 琥珀酸脱氢酶铁硫蛋白(EC 1.3.99.1)17 18 RXA01535 GR00427 1354 2760 富马酸水合酶前体(EC 4.2.1.2.)19 20 RXA00517 GR00131 1407 2447 苹果酸脱氢酶(EC 1.11.37)(EC 1.1.1.82)21 22 RXA01350 GR00392 1844 2827 苹果酸脱氢酶(EC 1.1.1.37)EMB-途径核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO23 24 RXA02149 GR00639 17786 18754 葡萄糖酸激酶(EC 2.7.1.2)25 26 RXA01814 GR00515 2571 910 葡糖磷酸变位酶(EC 5.4.2.2.)/磷酸甘露糖酸变位酶(EC 5.4.2.8)27 28 RXN02803 VV0086 1 657 葡糖磷酸变位酶(EC 5.4.2.2.)/磷酸甘露糖酸变位酶(EC 5.4.2.8)29 30 F RXA02803 GR00784 1 400 葡糖磷酸变位酶(EC 5.4.2.2.)/磷酸甘露糖酸变位酶(EC 5.4.2.8)31 32 RXN03076 VV0043 1624 35 葡糖磷酸变位酶(EC 5.4.2.2.)/磷酸甘露糖酸变位酶(EC 5.4.2.8)33 34 F RXA02854 GR10002 1588 5 葡糖磷酸变位酶(EC 5.4.2.2.)/磷酸甘露糖酸变位酶(EC 5.4.2.8)35 36 RXA00511 GR00129 1 513 葡糖磷酸变位酶(EC 5.4.2.2.)/磷酸甘露糖酸变位酶(EC 5.4.2.8)37 38 RXN01365 VV0091 1476 103 葡糖磷酸变位酶(EC 5.4.2.2.)/磷酸甘露糖酸变位酶(EC 5.4.2.8)39 40 F RXA01365 GR00397 897 4 葡糖磷酸变位酶(EC 5.4.2.2.)/磷酸甘露糖酸变位酶(EC 5.4.2.8)41 42 RXA00098 GR00014 6525 8144 葡萄糖-6-磷酸异构酶(GPI)(EC 5.3.1.9)43 44 RXA01989 GR00578 1 630 葡萄糖-6-磷酸异构酶式A(GPIA)(EC 5.3.1.9)45 46 RXA00340 GR00059 1549 2694 磷酸甘油酸变位酶(EC 5.4.2.1)47 48 RXA02492 GR00720 2201 2917 磷酸甘油酸变位酶(EC 5.4.2.1)49 50 RXA00381 GR00082 1451 846 磷酸甘油酸变位酶(EC 5.4.2.1)51 52 RXA02122 GR00636 6511 5813 磷酸甘油酸变位酶(EC 5.4.2.1)53 54 RXA00206 GR00032 6171 5134 6-磷酸果糖激酶(EC 2.7.1.11)55 56 RXA01243 GR00359 2302 3261 1-磷酸果糖激酶(EC 2.7.1.56)57 58 RXA01882 GR00538 1165 2154 1-磷酸果糖激酶(EC 2.7.1.56)
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO59 60 RXA01702 GR00479 1397 366 二磷酸果糖醛缩酶(EC 4.1.2.13)61 62 RXA02258 GR00654 26451 27227 磷酸丙糖异构酶(EC 5.3.1.1)63 64 RXN01225 VV0064 6382 4943 甘油醛-3-磷酸脱氢酶(EC 1.2.1.12)65 66 F RXA01225 GR00354 5302 6741 甘油醛-3-磷酸脱氢酶同系物67 68 RXA02256 GR00654 23934 24935 甘油醛-3-磷酸脱氢酶(EC 1.2.1.12)69 70 RXA02257 GR00654 25155 26369 磷酸甘油酸激酶(EC 2.7.2.3)71 72 RXA00235 GR00036 2365 1091 烯醇化酶(EC 4.2.1.11)73 74 RXA01093 GR00306 1552 122 丙酮酸激酶(EC 2.7.1.40)75 76 RXN02675 VV0098 72801 70945 丙酮酸激酶(EC 2.7.1.40)77 78 F RXA02675 GR00754 2 364 丙酮酸激酶(EC 2.7.1.40)79 80 F RXA02695 GR00755 2949 4370 丙酮酸激酶(EC 2.7.1.40)81 82 RXA00682 GR00179 5299 3401 磷酸烯醇丙酮酸合酶(EC 2.7.9.2)83 84 RXA00683 GR00179 6440 5349 磷酸烯醇丙酮酸合酶(EC 2.7.9.2)85 86 RXN00635 VV0135 22708 20972 丙酮酸脱氢酶(细胞色素)(EC 1.2.2.2)87 88 F RXA02807 GR00788 88 552 丙酮酸脱氢酶(细胞色素)(EC 1.2.2.2)89 90 F RXA00635 GR00167 3 923 丙酮酸脱氨酶(细胞色素)(EC 1.2.2.2)91 92 RXN03044 VV0019 1391 2221 丙酮酸脱氢酶E1组分(EC 1.2.4.1)93 94 FRXA02852 GR00852 3 281 丙酮酸脱氢酶E1组分(EC 1.2.4.1)95 96 F RXA00268 GR00041 125 955 丙酮酸脱氢酶E1组分(EC 1.2.4.1)97 98 RXN03086 VV0049 2243 2650 丙酮酸脱氢酶E1组分(EC 1.2.4.1)99 100 F RXA02887 GR10022 411 4 丙酮酸脱氢酶E1组分(EC 1.2.4.1)101 102 RXN03043 VV0019 1 1362 丙酮酸脱氢酶E1组分(EC 1.2.4.1)103 104 F RXA02897 GR10039 1291 5 丙酮酸脱氢酶E1组分(EC 1.2.4.1)105 106 RXN03083 VV0047 88 1110 二氢硫辛酰胺脱氢酶(EC 1.8.1.4)107 108 F RXA02853 GR10001 89 1495 二氢硫辛酰胺脱氢酶(EC 1.8.1.4)109 110 RXA02259 GR00654 27401 30172 磷酸烯醇丙酮酸羧化酶(EC 4.1.1.31)111 112 RXN02326 VV0047 4500 5315 丙酮酸羧化酶(EC 6.4.1.1)113 114 F RXA02326 GR00668 5338 4523 丙酮酸羧化酶115 116 RXN02327 VV0047 3533 4492 丙酮酸羧化酶(EC 6.4.1.1)117 118 F RXA02327 GR00668 6305 5346 丙酮酸羧化酶119 120 RXN02328 VV0047 1842 3437 丙酮酸羧化酶(EC 6.4.1.1)121 122 F RXA02328 GR00668 7783 6401 丙酮酸羧化酶(EC 6.4.1.1)123 124 RXN01048 VV0079 12539 11316 苹果酸酶(EC 1.1.1.39)125 126 F RXA01048 GR00296 3 290 苹果酸酶(EC 1.1.1.39)127 128 F RXA00290 GR00046 4693 5655 苹果酸酶(EC 1.1.1.39)129 130 RXA02694 GR00755 1879 2820 L-乳酸脱氢酶(EC 1.1.1.27)131 132 RXN00296 VV0176 35763 38606 D-乳酸脱氢酶(细胞色素)(EC 1.1.2.4)133 134 F RXA00296 GR00048 3 2837 D-乳酸脱氢酶(细胞色素)(EC 1.1.2.4)135 136 RXA01901 GR00544 4158 5417 L-乳酸脱氢酶(细胞色素)(EC 1.1.2.3)137 138 RXN01952 VV0105 9954 11666 D-乳酸脱氢酶(EC 1.1.1.28)
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO139 140 F RXA01952 GR00562 1 216 D-乳酸脱氢酶(EC 1.1.1.28)141 142 F RXA01955 GR00562 4611 6209 D-乳酸脱氢酶(EC 1.1.1.28)143 144 RXA00293 GR0047 2645 1734 D-3-磷酸甘油酸脱氢酶(EC 1.1.1.95)145 146 RXN01130 VV0157 6138 5536 D-3-磷酸甘油酸脱氢酶(EC 1.1.1.95)147 148 F RXA01130 GR00315 2 304 D-3-磷酸甘油酸脱氢酶(EC 1.1.1.95)149 150 RXN03112 VV0085 509 6 D-3-磷酸甘油酸脱氢酶(EC 1.1.1.95)151 152 F RX01133 GR00316 568 1116 D-3-磷酸甘油酸脱氢酶(EC 1.1.1.95)153 154 RXN00871 VV0127 3127 2240 IOLB蛋白155 156 F RXA00871 GR00239 2344 3207 IOLB蛋白:D-果糖1,6-二磷酸=甘油酮-CC磷酸+D-甘油醛3-磷酸。157 158 RXN02829 VV0354 287 559 IOLS蛋白159 160 F RXA02829 GR00816 287 562 IOLS蛋白161 162 RXN01468 VV0019 7474 8298 NAGD蛋白163 164 F RXA01468 GR00422 1250 2074 推定N-甘油醛-2-磷酸转移酶165 166 RXA00794 GR00211 3993 2989 GLPX蛋白167 168 RXN02920 VV0213 6135 5224 D-3-磷酸甘油酸脱氢酶(EC 1.1.1.95)169 170 F RXA02379 GR00690 1390 686 D-3-磷酸甘油酸脱氢酶(EC 1.1.1.95)171 172 RXN02688 VV0098 59053 58385 磷酸甘油酸变位酶(EC 5.4.2.1)173 174 RXN03087 VV0052 3216 3428 丙酮酸羧化酶(EC 6.4.1.1)175 176 RXN03186 VV0377 310 519 丙酮酸脱氢酶E1组分(EC 1.2.4.1)177 178 RXN03187 VV0382 3 281 丙酮酸脱氢酶E1组分(EC 1.2.4.1)179 180 RXN02591 VV0098 14370 12541 磷酸烯醇丙酮酸羧激酶[GTP](EC 4.1.1.32)181 182 RXS01260 VV0009 3477 2296 支链α-酮酸脱氢酶复合物的硫辛酰胺脱氢酶组分(E3)(EC 1.8.1.4)183 184 RXS01261 VV0009 3703 3533 支链α-酮酸脱氢酶复合物的硫辛酰胺脱氢酶组分(E3)(EC 1.8.1.4)甘油代谢核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO185 186 RXA02640 GR00749 1400 2926 甘油激酶(EC 2.7.1.30)187 188 RXN01025 VV0143 5483 4488 甘油-3-磷酸脱氢酶(NAD(P)+)(EC 1.1.1.94)189 190 F RX01025 GR00293 939 1853 甘油-3-磷酸脱氢酶(NAD(P)+)(EC 1.1.1.94)191 192 RXA01851 GR00525 3515 1830 需氧甘油-3-磷酸脱氢酶(NAD(P)+)(EC1.1.99.5)193 194 RXA01242 GR00359 1526 2302 甘油-3-磷酸调节子阻抑蛋白195 196 RXA02288 GR00661 992 147 甘油-3-磷酸调节子阻抑蛋白197 198 RXN01891 VV0122 24949 24086 甘油-3-磷酸-结合周质蛋白前体199 200 F RXA01891 GR00541 1736 918 甘油-3-磷酸-结合周质蛋白前体201 202 RXA02414 GR00703 3808 3062 参与甘油代谢的未鉴定蛋白(果蝇rhomboid的同系物)203 204 RXN01580 VV0122 22091 22807 甘油磷酸二酯磷酸二酯酶
表1(续)乙酸代谢核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO205 206 RXA01436 GR00418 2547 1357 乙酸激酶(EC 2.7.2.1)207 208 RXA00686 GR00179 8744 7941 乙酸操纵子阻抑蛋白209 210 RXA00246 GR00037 4425 3391 乙醇脱氢酶(EC 1.1.1.1)211 212 RXA01571 GR00438 1360 1959 乙醇脱氢酶(EC 1.1.1.1)213 214 RXA01572 GR00438 1928 2419 乙醇脱氢酶(EC 1.1.1.1)215 216 RXA01758 GR00498 3961 2945 乙醇脱氢酶(EC 1.1.1.1)217 218 RXA02539 GR00726 11676 10159 乙醛脱氢酶(EC219 220 RXN03061 VV0034 108 437 乙醛脱氢酶(EC 1.2.1.3)221 222 RXN03150 VV0155 10678 10055 乙醛脱氢酶(EC 1.2.1.3)223 224 RXN01340 VV0033 3 860 乙醛脱氢酶(EC 1.2.1.3)225 226 RXN01498 VV0008 1598 3160 乙醛脱氢酶(EC 1.2.1.3)227 228 RXN02674 VV0315 15614 14163 乙醛脱氢酶(EC 1.2.1.3)229 230 RXN00868 VV0127 2230 320 乙酰乳酸合酶大亚单位(EC 4.1.3.18)231 232 RXN01143 VV0077 9372 8254 乙酰乳酸合酶大亚单位(EC 4.1.3.18)233 234 RXN01146 VV0264 243 935 乙酰乳酸合酶大亚单位(EC 4.1.3.18)235 236 RXN01144 VV0077 8237 7722 乙酰乳酸合酶小亚单位(EC 4.1.3.18)丁二醇、二乙酰和3-羟基丁酮形成核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO237 238 RXA02474 GR00715 8082 7309 (S,S)-丁烷-2,3-二醇脱氢酶(EC 1.1.1.76)239 240 RXA02453 GR00710 6103 5351 3-羟基丁酮(二乙酰)还原酶(EC 1.1.1.5)241 242 RXS01758 VV0112 27383 28399 乙醇脱氢酶(EC 1.1.1.1)HMP-循环核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO243 244 RXA02737 GR00763 3312 1771 葡萄糖-6-磷酸1-脱氢酶(EC 1.1.1.49)245 246 RXA02738 GR00763 4499 3420 转醛醇酶(EC 2.2.1.2)247 248 RXA02739 GR00763 6769 4670 转酮醇酶(EC 2.2.1.1)249 250 RXA00965 GR00270 1232 510 6-磷酸葡萄糖酸脱氢酶,脱羧(EC 1.1.1.44)251 252 RXN00999 VV0106 2817 1366 6-磷酸葡萄糖酸脱氢酶,脱羧(EC 1.1.1.44)253 254 F RXA00999 GR00283 3012 4448 6-磷酸葡萄糖酸脱氢酶,脱羧(EC 1.1.1.44)核苷酸糖转换核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO255 256 RXN02596 VV0098 48784 47582 UDP-吡喃半乳糖变位酶(EC 5.4.99.9)257 258 F RXA02596 GR00742 1 489 UDP-吡喃半乳糖变位酶(EC 5.4.99.9)
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO259 260 F RXA02642 GR00749 5383 5880 UDP-吡喃半乳糖变位酶(EC 5.4.99.9)261 262 RXA02572 GR00737 2 646 UDP-葡萄糖6-脱氢酶(EC1.1.1.22)263 264 RXA02485 GR00718 2345 3445 UDP-N-乙酰烯醇丙酮酰葡糖胺还原酶(EC 11.1.158)265 266 RXA01216 GR00352 2302 1202 UDP-N-乙酰葡糖胺焦磷酸化酶(EC 2.7.7.23)267 268 RXA01259 GR00367 987 130 UTP-葡萄糖-1-磷酸尿苷酰转移酶(EC 2.7.7.9)269 270 RXA02028 GR00616 573 998 UTP-葡萄糖-1-磷酸尿苷酰转移酶(EC 2.7.7.9)271 272 RXA01262 GR00367 8351 7191 GDP-甘露糖6-脱氢酶(EC1.1.1.132)273 274 RXA01377 GR00400 3935 5020 甘露糖-1-磷酸鸟苷酰转移酶(EC 2.7.7.13)275 276 RXA02063 GR00626 3301 4527 葡萄糖-1-磷酸腺苷酰转移酶(EC 2.7.7.27)277 278 RXN00014 VV0048 8848 9627 葡萄糖-1-磷酸胸苷酰转移酶(EC 2.7.7.24)279 280 FRXA00014 GR00002 4448 5227 葡萄糖-1-磷酸胸苷酰转移酶(EC 2.7.7.24)281 282 RXA01570 GR00438 427 1281 葡萄糖-1-磷酸胸苷酰转移酶(EC 2.7.7.24)283 284 RXA02666 GR00753 7260 6493 D-核糖醇-5-磷酸胞苷酰转移酶(EC 2.7.7.40)285 286 RXA00825 GR00222 222 1154 DTDP-葡萄糖4,6-脱水酶(EC 4.2.1.46)肌醇和核糖醇代谢核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO287 288 RXA01887 GR00539 4219 3209 肌醇2-脱氢酶(EC 1.1.1.18)289 290 RXN00013 VV0048 7966 8838 肌醇-1(或4)-一磷酸酶1(EC 3.1.3.25)291 292 F RXA00013 GR00002 3566 4438 肌醇-1(或4)-一磷酸酶1(EC 3.1.3.25)293 294 RXA01099 GR00306 6328 5504 肌醇一磷酸磷酸酶295 296 RXN01332 VV0273 579 4 肌醇2-脱氢酶(EC 1.1.1.18)297 298 F RXA01332 GR00388 552 4 肌醇2-脱氢酶(EC 1.1.1.18)299 300 RXA01632 GR00454 2338 3342 肌醇2-脱氢酶(EC 1.1.1.18)301 302 RXA01633 GR00454 3380 4462 肌醇2-脱氢酶(EC 1.1.1.18)303 304 RXN01406 VV0278 2999 1977 肌醇2-脱氢酶(EC 1.1.1.18)305 306 RXN01630 VV0050 48113 47037 肌醇2-脱氢酶(EC 1.1.1.18)307 308 RXN00528 VV0079 23406 22318 肌醇-1-磷酸合酶(EC 5.5.1.4)309 310 RXN03057 VV0028 7017 7688 肌醇2-脱氢酶(1.1.1.18)311 312 F RXA02902 GR10040 10277 10948 葡萄糖-果糖氧化还原酶前体(EC 1.1.99.28)313 314 RXA00251 GR00038 931 224 核糖醇2-脱氢酶(EC 1.1.1.56)关于糖的利用核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO315 316 RXN02654 VV0090 12206 13090 葡萄糖1-脱氢酶(EC 1.1.1.47)317 318 F RXA02654 GR00752 7405 8289 葡萄糖1-脱氢酶II(EC 1.1.1.47)319 320 RXN01049 VV0079 9633 11114 葡糖酸激酶(EC 2.7.1.12)321 322 F RXA01049 GR00296 1502 492 葡糖酸激酶(EC 2.7.1.12)
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO323 324 F RXA01050 GR00296 1972 1499 葡糖酸激酶(EC 2.7.1.12)325 326 RXA00202 GR00032 1216 275 D-核糖结合周质蛋白前体327 328 RXN00872 VV0127 6557 5604 果糖激酶(EC 2.7.1.4)329 330 F RXA00872 GR00240 565 1086 果糖激酶(EC 2.7.1.4)331 332 RXN00799 VV0009 58477 56834 周质β-葡糖苷酶/β-木糖苷酶前体(EC 3.2.1.21)(EC 3.2.1.37)333 334 F RXA00799 GR00214 1 1584 周质β-葡糖苷酶/β-木糖苷酶前体(EC 3.2.1.21)(EC 3.2.1.37)335 336 RXA00032 GR00003 12028 10520 甘露醇2-脱氢酶(EC 1.1.1.67)337 338 RXA02528 GR00725 6880 7854 果糖阻抑蛋白339 340 RXN00316 VV0006 7035 8180 假定氧化还原酶(EC1.1.1.-)341 342 F RXA00309 GR00053 316 5 葡萄糖-果糖氧化还原酶前体(EC 1.1.99.28)343 344 RXN00310 VV0006 6616 7050 葡萄糖-果糖氧化还原酶前体(EC 1.1.99.28)345 346 F RXA00310 GR00053 735 301 葡萄糖-果糖氧化还原酶前体(EC 1.1.99.28)347 348 RXA00041 GR00007 1246 5 蔗糖-6-磷酸水解酶(EC 3.2.1.26)349 350 RXA02026 GR00615 725 6 蔗糖-6-磷酸水解酶(EC 3.2.1.26)351 352 RXA02061 GR00626 1842 349 蔗糖-6-磷酸水解酶(EC 3.2.1.26)353 354 RXN01369 VV0124 595 1776 甘露糖-6-磷酸异构酶(EC 5.3.1.8)355 356 F RXA01369 GR00398 3 503 甘露糖-6-磷酸异构酶(EC 5.3.1.8)357 358 F RXA01373 GR00399 595 1302 甘露糖-6-磷酸异构酶(EC 5.3.1.8)359 360 RXA02611 GR00743 1 1752 1,4-α-葡聚糖分支酶(EC 2.4.1.18)361 362 RXA02612 GR00743 1793 3985 1,4-α-葡聚糖分支酶(EC 2.4.1.18)363 364 RXN01884 VV0184 1 1890 糖元脱支酶(EC 2.4.1.25)(EC 3.2.1.33)365 366 F RXA01884 GR00539 3 1475 糖元脱支酶(EC 2.4.1.25)(EC 3.2.1.33)367 368 RXA01111 GR00306 16981 17427 糖元操纵子蛋白GLGX(EC 3.2.1.-)369 370 RXN01550 VV0143 14749 16260 糖元磷酸化酶(EC 2.4.1.1)371 372 F RXA01550 GR00431 3 1346 糖元磷酸化酶(EC 2.4.1.1)373 374 RXN02100 VV0318 2 2326 糖元磷酸化酶(EC 2.4.1.1)375 376 F RXA02100 GR00631 3 920 糖元磷酸化酶(EC 2.4.1.1)377 378 F RXA02113 GR00633 2 1207 糖元磷酸化酶(EC 2.4.1.1)379 380 RXA02147 GR00639 15516 16532 α-淀粉酶(EC 3.2.1.1)381 382 RXA01478 GR00422 10517 12352 葡糖淀粉酶G1和G2前体(EC 3.2.1.3)383 384 RXA01888 GR00539 4366 4923 葡萄糖抗性淀粉酶调节剂385 386 RXN0 1927 VV0127 50623 49244 木酮糖激酶(EC 2.7.1.17)387 388 FRXA01927 GR00555 3 1118 木酮糖激酶(EC 2.7.1.17)389 390 RXA02729 GR00762 747 4 核糖激酶(EC 2.7.1.15)391 392 RXA02797 GR00778 1739 2641 核糖激酶(EC 2.7.1.15)393 394 RXA02730 GR00762 1768 731 核糖操纵子阻抑蛋白395 396 RXA02551 GR00729 2193 2552 6-磷酸-β-葡糖苷酶(EC 3.2.1.86)397 398 RXA01325 GR00385 5676 5005 脱氧核糖-磷酸醛缩酶(EC 4.1.2.4)399 400 RXA00195 GR00030 543 1103 1-脱氧-D-木酮糖5-磷酸还原异构酶(EC 1.1.1.-)401 402 RXA00196 GR00030 1094 1708 1-脱氧-D-木酮糖5-磷酸还原异构酶(EC 1.1.1.-)
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO403 404 RXN01562 VV0191 1230 3137 1-脱氧木酮糖-5-磷酸合酶405 406 F RXA01562 GR00436 2 1039 1-脱氧木酮糖-5-磷酸合酶407 408 F RXA01705 GR00480 971 1573 1-脱氧木酮糖-5-磷酸合酶409 410 RXN00879 VV0099 8763 6646 4-α-葡聚糖转移酶(EC 2.4.1.25)411 412 F RXA00879 GR00242 5927 3828 4-α-葡聚糖转移酶(EC 2.4.1.25),淀粉麦芽糖酶413 414 RXN00043 VV0119 3244 2081 N-乙酰葡糖胺-6-磷酸去乙酰酶(EC 3.5.1.25)415 416 F RXA00043 GR00007 3244 2081 N-乙酰葡糖胺-6-磷酸去乙酰酶(EC 3.5.1.25)417 418 RXN01752 VV0127 35265 33805 N-乙酰葡糖胺转移酶(EC 2.4.1.-)419 420 F RXA01839 GR00520 1157 510 N-乙酰葡糖胺转移酶(EC 2.4.1-)421 422 RXA01859 GR00529 1473 547 N-乙酰葡糖胺转移酶(EC 2.4.1-)423 424 RXA00042 GR00007 2037 1279 葡糖胺-6-磷酸异构酶(EC 5.3.1.10)425 426 RXA01482 GR00422 17271 15397 葡糖胺-果糖-6-磷酸氨基转移酶(异构化)(EC 2.6.1.16)427 428 RXN03179 VV0336 2 667 糖醛酸异构酶(EC 5.3.1.12)429 430 F RXA02872 GR10013 675 4 糖醛酸异构酶,葡糖醛酸异构酶(EC 5.3.1.12)431 432 RXN03180 VV0337 672 163 糖醛酸异构酶(EC 5.3.1.12)433 434 F RXA02873 GR10014 672 163 糖醛酸异构酶,葡糖醛酸异构酶(EC 5.3.1.12)435 436 RXA02292 GR00662 1611 2285 半乳糖苷O-乙酰转移酶(EC 2.3.1.18)437 438 RXA02666 GR00753 7260 6493 D-核糖醇-5-磷酸胞苷酰转移酶(EC 2.7.7.40)439 440 RXA00202 GR00032 1216 275 D-核糖-结合周质蛋白前体441 442 RXA02440 GR00709 5097 4258 D-核糖-结合周质蛋白前体443 444 RXN01569 VV0009 41086 42444 dTDP-4-脱氢鼠李糖还原酶(EC 1.1.1.133)445 446 F RXA01569 GR00438 2 427 DTDP4-脱氢鼠李糖还原酶(EC 1.1.1.133)447 448 F RXA02055 GR00624 7122 8042 DTDP-4-脱氢鼠李糖还原酶(EC 1.1.1.133)449 450 RXA00825 GR00222 222 1154 DTDP-葡萄糖4,6-脱水酶(EC 4.2.1.46)451 452 RXA02054 GR00624 6103 7119 DTDP-葡萄糖4,6-脱水酶(EC 4.2.1.46)453 454 RXN00427 VV0112 7004 6219 dTDP-鼠李糖转移酶RFBF(EC 2.-.-.-)455 456 F RXA00427 GR00098 1591 2022 DTDP-鼠李糖转移酶RFBF(EC 2.-.-.-)457 458 RXA00327 GR00057 10263 9880 蛋白质ARAJ459 460 RXA00328 GR00057 11147 10656 蛋白质ARAJ461 462 RXA00329 GR00057 12390 11167 蛋白质ARAJ463 464 RXN01554 VV0135 28686 26545 葡聚糖内切-1.3-β-葡糖苷酶A1前体(EC 3.2.1.39)465 466 RXN03015 VV0063 289 8 UDP-葡萄糖6-脱氢酶(EC 1.1.1.22)467 468 RXN03056 VV0028 6258 6935 推定己酮糖-6-磷酸异构酶(EC 5.-.-.-)469 470 RXN03030 VV0009 57006 56443 周质β-葡糖苷酶/β-木糖苷酶前体(EC 3.2.1.21)(EC 3.2.1.37)471 472 RXN00401 VV0025 12427 11489 5-脱氢-4-脱氧葡糖二酸脱水酶(EC 4.2.1.41)473 474 RXN02125 VV0102 23242 22442 醛糖还原酶(EC 1.1.1.21)475 476 RXN00200 VV0181 1679 5116 阿拉伯糖转移酶亚单位B(EC 2.4.2.-)477 478 RXN01175 VV0017 39688 38303 磷酸-2-脱氢-3-脱氧庚糖酸醛缩酶(EC 4.1.2.15)479 480 RXN01376 VV0091 5610 4750 推定糖基转移酶WBIF
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO481 482 RXN01631 VV0050 47021 46143 推定己糖-6-磷酸异构酶(EC 5.-.-.-)483 484 RXN01593 VV0229 13274 12408 NAGD蛋白质485 486 RXN00337 VV0197 20369 21418 半乳糖激酶(EC 2.7.1.6)487 488 RXS00584 VV0323 5516 6640 磷酸-2-脱氢-3-脱氧庚糖酸醛缩酶(EC 4.1.2.15)489 490 RXS02574 β-己糖胺酶A前体(EC 3.2.1.52)491 492 RXS03215 葡萄糖-果糖氧化还原酶前体(EC 1.1.99.28)493 494 F RXA01915 GR00549 1 1008 葡萄糖-果糖氧化还原酶前体(EC 1.1.99.28)495 496 RXS03224 环麦芽糖糊精酶(EC 3.2.1.54)497 498 F RXA00038 GR00006 1417 260 环麦芽糖糊精酶(EC 3.2.1.54)499 500 RXC00233 参与糖代谢的蛋白质501 502 RXC00236 参与糖代谢的膜脂蛋白503 504 RXC00271 参与核糖代谢的运出蛋白505 506 RXC00338 参与糖代谢的蛋白质507 508 RXC00362 参与二醇代谢的跨膜蛋白509 510 RXC00412 参与糖代谢氨基酸ABC转运蛋白ATP结合蛋白511 512 RXC00526 参与糖代谢的ABC转运蛋白ATP结合蛋白513 514 RXC01004 参与糖代谢的跨膜蛋白515 516 RXC01017 参与糖代谢的胞质蛋白517 518 RXC01021 参与糖和硫胺素代谢的胞质激酶519 520 RXC01212 参与糖代谢的ABC转运蛋白ATP结合蛋白521 522 RXC01306 参与糖代谢的跨膜蛋白523 524 RXC01366 参与糖代谢的胞质蛋白525 526 RXC01372 参与糖代谢的胞质蛋白527 528 RXC01659 参与糖代谢的蛋白529 530 RXC01663 参与糖代谢的蛋白531 532 RXC01693 参与糖代谢的蛋白533 534 RXC01703 参与糖代谢的胞质蛋白535 536 RXC02254 参与糖代谢的膜结合蛋白537 538 RXC02255 参与糖代谢的胞质蛋白539 540 RXC02435 参与糖代谢的蛋白541 542 F RXA02435 GR00709 825 268 参与甘油代谢的未特征鉴定蛋白(果蝇rhomboid同系物)543 544 RXC03216 参与糖代谢的蛋白TCA-循环核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO545 546 RXA02175 GR00641 10710 9418 柠檬酸合酶(EC 4.1.3.7)547 548 RXA02621 GR00746 2647 1829 柠檬酸裂合酶β链(EC 4.1.3.6)
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO549 550 RXN00519 VV0144 5585 3372 异柠檬酸脱氢酶(NADP)(EC 1.1.1.42)551 552 F RXA00521 GR00133 2 1060 异柠檬酸脱氢酶(NADP)(EC 1.1.1.42)553 554 RXN02209 VV0304 1 1671 乌头酸水合酶(EC 4.2.1.3)555 556 F RXA02209 GR00648 3 1661 乌头酸水合酶(EC 4.2.1.3)557 558 RXN02213 VV0305 1378 2151 乌头酸水合酶(EC 4.2.1.3)559 560 F RXA02213 GR00649 1330 2046 乌头酸水合酶(EC 4.2.1.3)561 562 RXA02056 GR00625 3 2870 2-氧代戊二酸脱氢酶E1组分(EC 1.2.4.2)563 564 RXA01745 GR00495 2 1495 2-氧代戊二酸脱氢酶复合物的二氢硫辛酰胺琥珀酰转移酶组分(E2)
(EC 2.3.1.61)565 566 RXA00782 GR00206 3984 3103 琥珀酰-CoA合成酶α链(EC 6.2.1.5)567 568 RXA00783 GR00206 5280 4009 琥珀酰-CoA合成酶β链(EC 6.2.1.5)569 570 RXN01695 VV0139 11307 12806 L-苹果酸脱氢酶(受体)(EC 1.1.99.16)571 572 F RXA01615 GR00449 8608 9546 L-苹果酸脱氢酶(受体)(EC 1.1.99.16)573 574 F RXA01695 GR00474 4388 4179 L-苹果酸脱氢酶(受体)(EC 1.1.99.16)575 576 RXA00290 GR00046 4693 5655 苹果酸酶(EC 11.1.39)577 578 RXN01048 VV0079 12539 11316 苹果酸酶(EC 11.1.39)579 580 F RXA01048 GR00296 3 290 苹果酸酶(EC 11.1.39)581 582 F RXA00290 GR00046 4693 5655 苹果酸酶(EC 11.1.39)583 584 RXN03101 VV0066 2 583 2-氧代戊二酸脱氢酶复合物的二氢硫辛酰胺琥珀酰转移酶组分(E2)
(EC 2.3.1.61)585 586 RXN02046 VV0025 15056 14640 2-氧代戊二酸脱氢酶复合物的二氢硫辛酰胺琥珀酰转移酶组分(EC
2.3.1.61)587 588 RXN00389 VV0025 11481 9922 氧代戊二酸半醛脱氢酶(EC 1.2.1.-)乙醛酸旁路核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO589 590 RXN02399 VV0176 19708 18365 异柠檬酸裂合酶(EC 4.1.3.1)591 592 F RXA02399 GR00699 478 1773 异柠檬酸裂合酶(EC 4.1.3.1)593 594 RXN02404 VV0176 20259 22475 苹果酸合酶(EC 4.1.3.2)595 596 F RXA02404 GR00700 3798 1663 苹果酸合酶(EC 4.1.3.2)597 598 RXA01089 GR00304 3209 3958 乙醛酸诱导蛋白599 600 RXA01886 GR00539 3203 2430 乙醛酸诱导蛋白柠檬酸甲酯途径核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO600 602 RXN03117 VV0092 3087 1576 2-甲基异柠檬酸合酶(EC 5.3.3.-)601 604 F RXA00406 GR00090 978 4 2-甲基异柠檬酸合酶(EC 5.3.3.-)603 606 F RXA00514 GR00130 1983 1576 2-甲基异柠檬酸合酶(EC 5.3.3.-)605 608 RXA00512 GR00130 621 4 2-甲基柠檬酸合酶(EC 4.1.3.31)
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NQ SEQ ID NO607 610 RXA00518 GR00131 3069 2773 2-甲基柠檬酸合酶(EC 4.1.3.31)609 612 RXA01077 GR00300 4647 6017 2-甲基异柠檬酸合酶(EC 5.3.3.-)611 614 RXN03144 VV0141 2 901 2-甲基异柠檬酸合酶(EC 5.3.3.-)613 616 F RXA02322 GR00668 415 5 2-甲基异柠檬酸合酶(EC 5.3.3.-)615 618 RXA02329 GR00669 607 5 2-甲基异柠檬酸合酶(EC 5.3.3.-)617 620 RXA02332 GR00671 1906 764 2-甲基柠檬酸合酶(EC 4.1.3.31)619 622 RXN02333 VV0141 901 1815 异柠檬酸甲酯裂合酶(EC 4.1.3.30)621 624 F RXA02333 GR00671 2120 1902 异柠檬酸甲酯裂合酶(EC 4.1.3.30)623 626 RXA00030 GR00003 9590 9979 乳酰谷胱甘肽裂合酶(EC 4.4.1.5)甲基-丙二酰-CoA-变位酶核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO625 628 RXN00148 VV0167 9849 12059 甲基丙二酰-CoA变位酶α-亚单位(EC 5.4.99.2)627 630 F RXA00148 GR00023 2002 5 甲基丙二酰-CoA变位酶α-亚单位(EC 5.4.99.2)629 632 RXA00149 GR00023 3856 2009 甲基丙二酰-CoA变位酶β-亚单位(EC 5.4.99.2)其它核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO631 634 RXN00317 VV0197 26879 27532 磷酸乙醇酸磷酸酶(EC 3.1.3.18)635 636 F RXA00317 GR00055 344 6 磷酸乙醇酸磷酸酶(EC 3.1.3.18)637 638 RXA02196 GR00645 3956 3264 磷酸乙醇酸磷酸酶(EC 3.1.3.18)639 640 RXN02461 VV0124 14236 14643 磷酸乙醇酸磷酸酶(EC 3.1.3.18)氧化还原链核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO641 642 RXN01744 VV0174 2350 812 细胞色素D泛醌醇氧化酶亚单位I(EC 1.10.3.-)643 644 F RXA00055 GR00008 11753 11890 细胞色素D泛醌醇氧化酶亚单位I(EC 1.10.3.-)645 646 F RXA01744 GR00494 2113 812 细胞色素D泛醌醇氧化酶亚单位I(EC 1.10.3.-)647 648 RXA00379 GR00082 212 6 细胞色素C型生物发生蛋白CCDA649 650 RXA00385 GR00083 773 435 细胞色素C型生物发生蛋白CCDA651 652 RXA01743 GR00494 806 6 细胞色素D泛醌醇氧化酶亚单位II(EC 1.10.3-)653 654 RXN02480 VV0084 31222 29567 细胞色素C氧化酶多肽I(EC 1.9.3.1)655 656 F RXA01919 GR00550 288 4 细胞色素C氧化酶亚单位I(EC 1.9.3.1)657 658 FRXA02480 GR00717 1449 601 细胞色素C氧化酶多肽I(EC 1.9.3.1)659 660 F RXA02481 GR00717 1945 1334 细胞色素C氧化酶多肽I(EC 1.9.3.1)
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO661 662 RXA02140 GR00639 7339 8415 细胞色素C氧化酶多肽II(EC 1.9.3.1)663 664 RXA02142 GR00639 9413 10063 细胞色素C氧化酶多肽I(EC 1.9.3.1)665 666 RXA02144 GR00639 11025 12248 RIESKE氏铁硫蛋白667 668 RXA02740 GR00763 7613 8542 可能的细胞色素C氧化酶装配因子669 670 RXA02743 GR00763 13534 12497 细胞色素M3控制蛋白671 672 RXA01227 GR00355 1199 1519 铁氧化还原蛋白673 674 RXA01865 GR00532 436 122 铁氧化还原蛋白675 676 RXA00680 GR00179 2632 2315 铁氧化还原蛋白VI677 678 RXA00679 GR00179 2302 1037 铁氧化还原蛋白-NAD(+)还原酶(EC1.18.1.3)679 680 RXA00224 GR00032 24965 24015 电子转移黄素蛋白α-亚单位681 682 RXA00225 GR00032 25783 24998 电子转移黄素蛋白β-亚单位683 684 RXN00606 VV0192 11299 9026 NADH脱氢酶I链L(EC 1.6.5.3)685 686 FRXA00606 GR00160 121 1869 NADH脱氢酶I链L(EC 1.6.5.3)687 688 RXN00595 VV0192 8642 7113 NADH脱氢酶I链M(EC 1.6.5.3)689 690 FRXA00608 GR00160 2253 3017 NADH脱氢酶I链M(EC 1.6.5.3)691 692 RXA00913 GR00249 3 2120 NADH脱氢酶I链L(EC 1.6.5.3)693 694 RXA00909 GR00247 2552 3406 NADH脱氢酶I链L(EC 1.6.5.3)695 696 RXA00700 GR00182 846 43 NADH-泛醌氧化还原酶链2697 698 RXN00483 VV0086 44824 46287 NADH-泛醌氧化还原酶39 KD亚单位前体(EC 1.6.5.3)(EC 1.6.99.3)699 700 F RXA00483 GR00119 19106 20569 NADH-泛醌氧化还原酶39 KD亚单位前体(EC 1.6.5.3)(EC 1.6.99.3)701 702 RXA01534 GR00427 1035 547 NADH依赖性FMN氧化还原酶703 704 RXA00288 GR00046 2646 1636 醌氧化还原酶(EC 1.6.5.5)705 706 RXA02741 GR00763 9585 8620 醌氧化还原酶(EC 1.6.5.5)707 708 RXN02560 VV0101 9922 10788 NADPH-黄素氧化还原酶(EC 1.6.99.-)709 710 F RXA02560 GR00731 6339 7160 NADPH-黄素氧化还原酶(EC 1.6.99.-)711 712 RXA01311 GR00380 1611 865 琥珀酸脱氢酶铁硫蛋白(EC 1.3.99.1)713 714 RXN03014 VV0058 1273 368 NADH脱氢酶I链M(EC 1.6.5.3)715 716 F RXA00910 GR00248 3 1259 氢化酶亚单位717 718 RXN01895 VV0117 955 5 NADH脱氢酶(EC 1.6.99.3)719 720 F RXA01895 GR00543 2 817 脱氢酶721 722 RXA00703 GR00183 2556 271 甲酸脱氢酶α链(EC 1.2.1.2)723 734 RXN00705 VV0005 6111 5197 FDHD蛋白725 726 F RXA00705 GR00184 1291 407 FDHD蛋白727 728 RXN00388 VV0025 2081 3091 细胞色素生物发生蛋白CCSA729 730 F RXA00388 GR00085 969 667 类似于细胞色素c的必需蛋白731 732 F RXA00386 GR00084 514 5 RESC蛋白,类似于细胞色素c生物发生蛋白的必需蛋白733 734 RXA00945 GR00259 1876 2847 推定的细胞色素氧化酶735 736 RXN02556 VV0101 5602 6759 黄素血红素蛋白/二氢蝶啶还原酶(EC 1.6.99.7)
表1(续)核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO737 738 F RXA02556 GR00731 2019 3176 黄素血红素蛋白739 740 RXA01392 GR00408 2297 3373 谷胱苷肽S-转移酶(EC 2.5.1.18)741 742 RXA00800 GR00214 2031 3134 谷胱苷肽依赖性甲醛脱氢酶(EC 1.2.1.1)743 744 RXA02143 GR00639 10138 11025 QCRC蛋白:甲萘醌醇:细胞色素c氧化还原酶745 746 RXN03096 VV0058 405 4 NADH脱氢酶I链M(EC 1.6.5.3)747 748 RXN02036 VV0176 32683 33063 NADH-泛醌氧化还原酶链4(EC 1.6.5.3)749 750 RXN02765 VV0317 3552 2794 假定氧化还原酶751 752 RXN02206 VV0302 1784 849 假定氧化还原酶753 754 RXN02554 VV0101 4633 4010 假定氧化还原酶(EC 1.1.1.-)ATP-合酶核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO755 756 RXN01204 VV0121 1270 461 ATP合酶A链(EC 3.6.1.34)757 758 FRXA01204 GR00345 394 1155 ATP合酶A链(EC 3.6.1.34)759 760 RXA01201 GR00344 675 2315 ATP合酶α链(EC 3.6.1.34)761 762 RXN01193 VV0175 5280 3832 ATP合酶β链(EC 3.6.1.34)763 764 F RXA01193 GR00343 15 755 ATP合酶β链(EC 3.6.1.34)765 766 F RXA01203 GR00344 3355 3993 ATP合酶β链(EC 3.6.1.34)767 768 RXN02821 VV0121 324 85 ATP合酶C链(EC 3.6.1.34)769 770 F RXA02821 GR00802 139 318 ATP合酶C链(EC 3.6.1.34)771 772 RXA01200 GR00344 2 610 ATP合酶δ链(EC 3.6.1.34)773 774 RXA01194 GR00343 770 1141 ATP合酶ε链(EC 3.6.1.34)775 776 RXA01202 GR00344 2375 3349 ATP合酶γ链(EC 3.6.1.34)777 778 RXN02434 VV0090 4923 3274 ATP结合蛋白细胞色素代谢核酸 氨基酸 标识码 重叠群 NT起始 NT终止 功能SEQ ID NO SEQ ID NO779 780 RXN00684 VV0005 29864 28581 细胞色素P450116(EC 1.14.-.-)781 782 RXN00387 VV0025 1150 2004 假定细胞色素c生物发生蛋白
表2-排除的基因 | |||
GenBankTM登记号 | 基因名称 | 基因功能 | 参考文献 |
A09073 | ppg | 磷酸烯醇丙酮酸羧化酶 | Bachmann,B.等“编码磷酸烯醇丙酮酸羧化酶的DNA片段、携带所述片段的重组DNA、带有所述重组DNA的菌株和采用所述菌株生产L-氨基酸的方法”,专利:EP 0358940-A 03/21/90 |
A45579,A45581,A45583,A45585A45587 | 苏氨酸脱水酶 | Moeckel,B.等“利用具有去调节苏氨酸脱水酶的重组微生物生产L-异亮氨酸”专利:WO 9519442-A 5 07/20/95 | |
AB003132 | murC;ftsQ;ftsZ | Kobayashi,M.等“棒杆菌ftsZ乙基因的克隆、测序和鉴定”Biochem.Biophys.Res.Commun.,236(2):383-388(1997) | |
AB015023 | murC;ftsQ | Wachi,M.等“棒杆菌的marC基因”Appl.Microbiol.Biotechnol.,51(2):223-228(1999) | |
AB018530 | dtsR | Kimura,E.等“对拯救衍生自乳发酵短杆菌的突变体的去垢剂敏感性的新型基因dtsR的分子克隆”,Biosci.Biotechnol.Biochem.,60(10):1565-1570(1996) | |
AB018531 | dtsR1;dtsR2 | ||
AB020624 | murl | D-谷氨酸消旋酶 | |
AB023377 | tkt | 转羟乙醛酶 |
表2(续) | |||
AB024708 | gltB;gltD | 谷氨酰胺2-酮戊二酸氨基转移酶大亚基和小亚基 | |
AB025424 | acn | 顺乌头酸酶 | |
AB027714 | rep | 复制蛋白 | |
AB027715 | rep;aad | 复制蛋白;氨基糖苷腺苷酸转移酶 | |
AF005242 | argC | N-乙酰谷氨酸-5-半醛脱氢酶 | |
AF005635 | glnA | 谷氨酰胺合成酶 | |
AF030405 | hisF | 环化酶 | |
AF030520 | argG | 精氨琥珀酸合成酶 | |
AF031518 | argF | 鸟氨酸氨甲酰转移酶 | |
AF036932 | aroD | 3-脱氢奎尼酸脱水酶 | |
AF038548 | pyc | 丙酮酸羧化酶 | |
AF038651 | dciAE;apt;rel | 二肽结合蛋白;腺嘌呤磷酸核糖转移酶;GTP焦磷酸激酶 | Wehmeier,L.等“谷氨酸棒杆菌rel基因在(p)ppGpp代谢中的作用”,Microbiology,144:1853-1862(1998) |
AF041436 | argR | 精氨酸阻抑蛋白 | |
AF045998 | impA | 肌醇单磷酸磷酸酶 | |
AF048764 | argH | 精氨琥珀酸裂合酶 |
表2(续) | |||
AF049897 | argC;argJ;argB;argD;argF;argR;argG;argH | N-乙酰谷氨酰磷酸还原酶;鸟氨酸乙酰基转移酶;N-乙酰谷氨酸激酶;乙酰鸟氨酸转氨酶;鸟氨酸氨甲酰转移酶;精氨酸阻抑蛋白;精氨琥珀酸合酶;精氨琥珀酸裂合酶 | |
AF050109 | inhA | 烯醇-酰基载体蛋白还原酶 | |
AF050166 | hisG | ATP磷酸核糖基转移酶 | |
AF051846 | hisA | 磷酸核糖甲酰亚氨基-5-氨基-1-磷酸核糖基-4-咪唑甲酰胺异构酶 | |
AF052652 | metA | 高丝氨酸O-乙酰基转移酶 | Park,S.等“metA-一种谷氨酸棒杆菌中编码高丝氨酸乙酰基转移酶的甲硫氨酸生物合成基因的分离与分析”,Mol.Cell.,8(3):286-294(1998) |
AF053071 | aroB | 脱氢奎尼酸合成酶 | |
AF060558 | hisH | 谷氨酰胺转酰胺酶 | |
AF086704 | hisE | 磷酸核糖基-ATP-焦磷酸水解酶 | |
AF114233 | aroA | 5-烯醇丙酮酸莽草酸3-磷酸合酶 | |
AF116184 | panD | L-天冬氨酸-α-脱羧酶前体 | Dusch,N.等“编码L-天冬氨酸-α-脱羧酶的谷氨酸棒杆菌panD基因的表达导致大肠杆菌过度产生泛酸”,Appl.Environ.Microbiol.,65(4)1530-1539(1999) |
AF124518 | aroD;aroE | 3-脱氢奎尼酸酶(dehydroquinase);莽草酸脱氢酶 |
表2(续) | |||
AF124600 | aroC;aroK;aroB;pepQ | 分支酸合酶;莽草酸激酶;3-脱氢奎尼酸合酶;推定的胞质肽酶 | |
AF145897 | inhA | ||
AF145898 | inhA | ||
AJ001436 | ectP | ectoine、甜菜碱、脯氨酸的转运 | Peter,H.等“谷氨酸棒杆菌备有四种匹配溶质的第二载体:脯氨酸/ectoine摄入系统ProP和ectoine/脯氨酸/甜菜碱载体EctP的鉴定、测序和表征”,J.Bacteriol.,180(22):6005-6012(1998) |
AJ004934 | dapD | 四氢2,6-吡啶二羧酸琥珀酰化酶(不完整的i) | Wehrmann,A.等“不同模式的二氨基庚二酸合成及其在细胞壁完整性方面的作用:用谷氨酸棒杆菌进行的研究”,J.Bacteriol.,180(12);3159-3165(1998) |
AJ007732 | ppc;secG;amt;ocd;soxA | 磷酸烯醇丙酮酸羧化酶;?;高亲合性铵摄入蛋白;推定的鸟氨酸-环脱羧酶;肌氨酸氧化酶 | |
AJ010319 | ftsY,glnB,glnD;srp;amtP | 参与细胞分裂;PII蛋白;尿苷酰基转移酶(尿苷酰基释去酶);信号识别颗粒;低亲合性铵摄入蛋白 | Jakoby,M.等“谷氨酸棒杆菌中的氮调节;涉及相应蛋白质的生物化学鉴定的基因的分离”,FEMS Microbiol.,173(2):303-310(1999) |
AJ132968 | cat | 氯霉素乙酰基转移酶 | |
AJ224946 | mqo | L-苹果酸;醌氧化还原酶 | Molenaar,D.等“谷氨酸棒杆菌膜相关苹果酸脱氢酶(受体)的生物化学和遗传学特征鉴定”,Eur.J.Biochem.,254(2):395-403(1998) |
AJ238250 | ndh | NADH脱氢酶 |
表2(续) | |||
AJ238703 | porA | 膜孔蛋白 | Lichtinger,T.等“谷氨酸棒杆菌细胞壁膜孔蛋白的生物化学和生物物理特征鉴定:该通道由低分子量多肽形成”,Biochemistry,37(43):15024-15032(1998) |
D17429 | 转座因子IS31831 | Vertes等“得自谷氨酸棒杆菌的转座因子IS31831的分离和特征鉴定”,Mol.Microbiol.,11(4):739-746(1994) | |
D84102 | odhA | 2-酮戊二酸脱氢酶 | Usuda,Y.等“编码新型2-酮戊二酸脱氢酶的谷氨酸棒杆菌(乳发酵短杆菌AJ12036)odhA基因的分子克隆”,Microbiology,142:3347-3354(1996) |
E01358 | hdh;hk | 高丝氨酸脱氢酶;高丝氨酸激酶 | Katsumata,R.等“L-苏氨酸和L-异亮氨酸的生产”,专利:JP 1987232392-A2 10/12/87 |
E01359 | 高丝氨酸激酶基因起始密码子的上游 | Katsumata,R.等“L-苏氨酸和L-异亮氨酸的生产”,专利t:JP 1987232392-A2 10/12/87 | |
E01375 | 色氨酸操纵子 | ||
E01376 | trpL;trpE | 前导肽;邻氨基苯甲酸合酶 | Matsui,K.等“色氨酸操纵子、由其编码的肽和蛋白质、色氨酸操纵子基因表达的利用和色氨酸的生产”,专利:JP 1987244382-A 1 10/24/87 |
E01377 | 色氨酸操纵子的启动子和操纵基因区 | Matsui,K.等“色氨酸操纵子、由其编码的肽和蛋白质,色氨酸操纵子基因表达的利用和色氨酸的生产”,专利:JP 1987244382-A 1 10/24/87 | |
E03937 | 生物素合酶 | Hatakeyama,K.等“含能够编码生物素合成酶的基因的DNA片段及其应用”,专利:JP 1992278088-A 1 10/02/92 | |
E04040 | 二氨基壬酸氨基转移酶 | Kohama,K.等“编码二氨基庚二酸氨基转移酶和脱硫生物素合成酶的基因及其应用”,专利:JP 1992330284-A 1 11/18/92 |
表2(续) | |||
E04041 | 脱硫生物素合成酶 | Kohama,K.等“编码二氨基庚二酸氨基转移酶和脱硫生物素合成酶的基因及其应用”,专利:JP1992330284-A 1 11/18/92 | |
E04307 | Flavum天冬氨酸酶 | Kurusu,Y.等“编码天冬氨酸酶的基因DNA及其应用”,专利:JP1993030977-A 1 02/09/93 | |
E04376 | 异柠檬酸裂合酶 | Katsumata,R.等“基因表现控制性DNA”,专利:JP 1993056782-A 3 03/09/93 | |
E04377 | 异柠檬酸裂合酶N末端片段 | Katsumata,R.等“基因表现控制性DNA”,专利:JP 1993056782-A 3 03/09/93 | |
E04484 | 预苯酸脱水酶 | Sotouchi,N.等“通过发酵生产L-苯丙氨酸”,专利:JP 1993076352-A203/30/93 | |
E05108 | 天冬氨酸激酶 | Fugono,N.等“编码天冬氨酸激酶的基因DNA及其应用”,专利:JP1993184366-A 1 07/27/93 | |
E05112 | 二氢-dipichorinate合成酶 | Hatakeyama,K.等“编码二氢2,6-吡啶二羧酸合成酶的基因DNA及其应用”,专利:JP 1993184371-A 1 07/27/93 | |
E05776 | 二氨基庚二酸脱氢酶 | Kobayashi,M.等“编码二氨基庚二酸脱氢酶的基因DNA及其应用”,专利:JP 1993284970-A 1 1 1/02/93 | |
E05779 | 苏氨酸合酶 | Kohama,K.等“编码苏氨酸合酶的基因DNA及其应用”,专利:JP1993284972-A 1 11/02/93 | |
E06110 | 预苯酸脱水酶 | Kikuchi,T.等“通过发酵法生产L-苯丙氨酸”,专利:JP 1993344881-A 112/27/93 | |
E06111 | 突变型预苯酸脱水酶 | Kikuchi,T.等“通过发酵法生产L-苯丙氨酸”,专利:JP 1993344881-A 112/27/93 |
表2(续) | |||
E06146 | 乙酰羟酸合成酶 | Inui,M.等“能够编码乙酰羟酸合成酶的基因及其应用”,专利:JP1993344893-A 1 12/27/93 | |
E06825 | 天冬氨酸激酶 | Sugimoto,M.等“突变型天冬氨酸激酶基因”,专利:JP 1994062866-A 103/08/94 | |
E06826 | 突变型天冬氨酸激酶α亚基 | Sugimoto,M.等“突变型天冬氨酸激酶基因”,专利:JP 1994062866-A 103/08/94 | |
E06827 | 突变型天冬氨酸激酶α亚基 | Sugimoto,M.等“突变型天冬氨酸激酶基因”,专利:JP 1994062866-A 103/08/94 | |
E07701 | secY | Honno,N.等“参与膜蛋白整合入膜的基因DNA”,专利:JP 1994169780-A 106/21/94 | |
E08177 | 天冬氨酸激酶 | Sato,Y.等“能够编码反馈抑制释放的天冬氨酸激酶的遗传DNA及其应用”,专利:JP 1994261766-A 1 09/20/94 | |
E08178,E08179,E08180,E08181,E08182 | 反馈抑制释放的天冬氨酸激酶 | Sato,Y.等“能够编码反馈抑制释放的天冬氨酸激酶的遗传DNA及其应用”,专利:JP 1994261766-A 1 09/20/94 | |
E08232 | 乙酰羟酸异构还原酶 | Inui,M.等“编码乙酰羟酸异构还原酶的基因DNA”,专利:JP 1994277067-A10/04/94 |
表2(续) | |||
E08234 | secE | Asai,Y.等“编码蛋白质转运机制的基因DNA”,专利:JP 1994277073-A 110/04/94 | |
E08643 | FT氨基转移酶和脱硫生物素合成酶启动子区 | Hatakeyama,K.等“棒杆菌中具有启动子功能的DNA片段”,专利:JP1995031476-A 1 02/03/95 | |
E08646 | 生物素合成酶 | Hatakeyama,K.等“棒杆菌中具有启动子功能的DNA片段”,专利:JP1995031476-A 1 02/03/95 | |
E08649 | 天冬氨酸酶 | Kohama,K.等“棒杆菌中具有启动子功能的DNA片段”,专利:JP1995031478-A 1 02/03/95 | |
E08900 | 二氢2,6-吡啶二羧酸还原酶 | Madori,M.等“含有编码二氢2,6-吡啶二羧酸还原酶的基因的DNA片段及其应用”,专利:JP 1995075578-A 1 03/20/95 | |
E08901 | 二氨基庚二酸脱羧酶 | Madori,M.等“含有编码二氢庚二酸脱羧酶的基因的DNA片段及其应用”,专利:JP 1995075579-A 1 03/20/95 | |
E12594 | 丝氨酸羟甲基转移酶 | Hatakeyama,K.等“L-色氨酸的生产”,专利:JP 1997028391-A 1 02/04/97 | |
E12760,E12759E12758 | 转座酶 | Moriya,M.等“采用人工转座子扩增基因”,专利:JP 1997070291-A 03/18/97 | |
E12764 | 精氨酰-tRNA合成酶;二氨基庚二酸脱羧酶 | Moriya,M.等“采用人工转座子扩增基因”,专利:JP 1997070291-A 03/18/97 | |
E12767 | 二氢2,6-吡啶二羧酸合成酶 | Moriya,M.等“采用人工转座子扩增基因”,专利:JP 1997070291-A 03/18/97 | |
E12770 | 天冬氨酸激酶 | Moriya,M.等“采用人工转座子扩增基因”,专利:JP 1997070291-A 03/18/97 |
表2(续) | |||
E12773 | 二氢2,6-吡啶二羧酸还原酶 | Moriya,M.等“采用人工转座子扩增基因”,专利:JP 1997070291-A 03/18/97 | |
E13655 | 葡萄糖-6-磷酸脱氢酶 | Hatakeyama,K.等“葡萄糖-6-磷酸脱氢酶以及能够编码其的DNA”,专利:JP 1997224661-A 1 09/02/97 | |
L01508 | IlvA | 苏氨酸脱水酶 | Moeckel,B.等“谷氨酸棒杆菌苏氨酸脱水酶的功能和结构分析”,J.Bacteriol.,174:8065-8072(1992) |
L07603 | EC 4.2.1.15 | 3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶 | Chen,C.等“谷氨酸棒杆菌3-脱氧-D-阿拉伯庚酮糖酸-7-磷酸合酶基因的克隆和核苷酸序列”,FEMS Microbiol. Lett.,107:223-230(1993) |
L09232 | IlvB;ilvN;ilvC | 乙酰羟酸合酶大亚基乙酰羟酸合酶小亚基;乙酰羟酸异构还原酶 | Keilhauer,C.等“谷氨酸棒杆菌中的异亮氨酸合成:ilvB-ilvN-ilvC操纵子的分子分析”,J.Bacteriol.,175(17):5595-5603(1993) |
L18874 | PtsM | 磷酸烯醇丙酮酸糖磷酸转移酶 | Fouet,A等“枯草杆菌磷酸转移酶系统的蔗糖特异性酶II:在大肠杆菌中的表达以及与肠细菌酶II的同源性”,PNAS USA,84(24):8773-8777(1987);Lee,J.K.等“编码谷氨酸棒杆菌甘露糖酶II的基因的核苷酸序列和推导的蛋白质序列的分析”,FEMS Microbiol.Lett.,119(1-2):137-145(1994) |
L27123 | aceB | 苹果酸合酶 | Lee,H-S.等“aceB-一种谷氨酸棒杆菌中编码苹果酸合酶的基因的分子特征鉴定”,J.Microbiol.Biotechnol.,4(4):256-263(1994) |
L27126 | 丙酮酸激酶 | Jetten,M.S.等“谷氨酸棒杆菌丙酮酸激酶的结构与功能分析”,Appl.Environ.Microbiol.,60(7):2501-2507(1994) | |
L28760 | aceA | 异柠檬酸裂合酶 | |
L35906 | dtxr | 白喉毒素阻抑蛋白 | Oguiza,J.A.等“来自乳发酵短杆菌的白喉棒杆菌dtxR的分子克隆、DNA序列分析和特征鉴定”,J.Bacteriol.,177(2):465-467(1995) |
表2(续) | |||
M13774 | 预苯酸脱水酶 | Follettie,M.T.等“谷氨酸棒杆菌pheA基因的分子克隆和核苷酸序列”,J.Bacteriol.,167:695-702(1986) | |
M16175 | 5S rRNA | Park,Y-H.等“通过56种rRNA序列对棒杆菌进行的种系发生分析”,J.Bacteriol.,169:1801-1806(1987) | |
M16663 | trpE | 邻氧基苯甲酸合酶,5’端 | Sano,K.等“一种谷氨酸生产细菌-乳发酵短杆菌trp操纵子控制区的结构和功能”,Gene,52:191-200(1987) |
M16664 | trpA | 色氨酸合酶,3’端 | Sano,K.等“一种谷氨酸生产细菌-乳发酵短杆菌trp操纵子控制区的结构和功能”,Gene,52:191-200(1987) |
M25819 | 磷酸烯醇丙酮酸羧化酶 | O’Regan,M.等“谷氨酸棒杆菌ATCC13032的磷酸烯醇丙酮酸羧化酶编码基因的克隆和核苷酸序列”,Gene,77(2):237-251(1989) | |
M85106 | 23S rRNA基因插入序列 | Roller,C.等“具有高DNAG+C含量的革兰氏阳性细菌的特征为在其23SrRNA基因内存在共同插入”,J.Gen.Microbiol.,138:1167-1175(1992) | |
M85107,M85108 | 23S rRNA基因插入序列 | Roller,C.等“具有高DNAG+C含量的革兰氏阳性细菌的特征为在其23SrRNA基因存在共同插入”,J.Gen.Microbiol.,138:1167-1175(1992) | |
M89931 | aecD;brnQ;yhbw | βC-S裂合酶;支链氨基酸摄入载体;假定的蛋白yhbw | Rossol,I.等“谷氨酸棒杆菌aecD基因编码一种具有降解氨乙基半胱氨酸的α,β-消除活性的C-S裂合酶”,J.Bacteriol.,174(9):2968-2977(1992);Tauch,A.等“谷氨酸棒杆菌ATCC 13032中的异亮氨酸摄入由brnQ基因产物控制”,Arch.Microbiol.,169(4):303-312(1998) |
表2(续) | |||
S59299 | trp | 前导基因(启动子) | Herry,D.M.等“克隆谷氨酸棒杆菌苏色氨酸高产菌株的trp基因簇:鉴定trp前导序列突变”,Appl.Environ.Microbiol.,59(3):791-799(1993) |
U11545 | trpD | 邻氨基苯甲酸磷酸核糖基转移酶 | O’Gara,J.P.和Dunican,L.K.(1994)“谷氨酸棒杆菌ATCC 21850 tpD基因的完整核苷酸序列”,Thesis,Microbiology Department,University CollegeGalway,Ireland. |
U13922 | cgIIM;cgIIR;clgIIR | 推定的II型5-胞嘧啶甲基转移酶;推定的II型限制性内切核酸酶;推定的I型或III型限制性内切核酸酶 | Schafer,A.等“来自谷氨酸棒杆菌ATCC 13032的编码胁迫敏感性限制系统的DNA区的克隆和特征鉴定及其在与大肠杆菌的属间接合方面的作用分析”,J.Bacteriol.,176(23):7309-7319(1994);“谷氨酸棒杆菌编码McrBC缺陷型大肠杆菌菌株中的5-胞嘧啶的cgIIM基因”,Gene,203(2):95-101(1997) |
U14965 | recA | ||
U131224 | ppx | Ankri,S.等“谷氨酸棒杆菌生物合成途径中的突变:proA步骤的天然旁路”,J.Bacteriol.,178(15):4412-4419(1996) | |
U31225 | proC | L-脯氨酸:NADP+5-氧化还原酶 | Ankri,S.等“谷氨酸棒杆菌生物合成途径中的突变:proA步骤的天然旁路”,J.Bacteriol.,178(15):4412-4419(1996) |
U31230 | obg;proB;unkdh | ?;γ-谷氨酰激酶;与D-异构体特异性的2-羟基酸脱水酶相似 | Ankri,S.等“谷氨酸棒杆菌生物合成途径中的突变:proA步骤的天然旁路”,J.Bacteriol.,178(15):4412-4419(1996) |
U31281 | bioB | 生物素合酶 | Serebriiskii,I.G.,“bio B超家族的两个新成员:Methylobacillus flagellatum和谷氨酸棒杆菌的bio B基因的克隆、测序和表达”,Gene,175:15-22(1996) |
表2(续) | |||
U35023 | thtR;accBC | 硫代硫酸硫转移酶;酰基CoA羧化酶 | Jager,W.等“编码类似于生物素羧化酶和生物素羧基载体蛋白的二结构域蛋白的谷氨酸棒杆菌基因”,Arch.Microbiol.,166(2);76-82(1996) |
U43535 | cmr | 多种药物抗性蛋白 | Jager,W.等“在异源宿主大肠杆菌中赋予多种药物抗性的谷氨酸棒杆菌基因”,J.Bacteriol.,179(7):2449-2451(1997) |
U43536 | clpB | 热激ATP结合蛋白 | |
U53587 | aphA-3 | 3’5”-氨基糖苷磷酸转移酶 | |
U89648 | 谷氨酸棒杆菌中参与组氨酸生物合成的未鉴定序列,部分序列 | ||
X04960 | TrpA;trpB;trpC;trpD;trpE;trpG;trpL | 色氨酸操纵子 | Matsui,K.等“乳发酵短杆菌色氨酸操纵子的完整核苷酸序列和推导的氨基酸序列”,Nucleic Acids Res.,14(24):10113-10114(1986) |
X07563 | lysA | DAP脱羧酶(中-二氨基庚二酸脱羧酶,EC 41.1.20) | Yeh,P.等“谷氨酸棒杆菌的lysA基因的核酸序列以及其表达调节的可能机制”,Mol.Gen.Genet.,212(1):112-119(1988) |
X14234 | EC 4.1.1.31 | 磷酸烯醇丙酮酸羧化酶 | Eikmanns,B.J.等“谷氨酸棒杆菌的磷酸烯醇丙酮酸羧化酶基因:分子克隆、核苷酸序列和表达”,Mol.Gen.Genet.,218(2):330-339(1989);Lepiniec,L.等“高梁磷酸烯醇丙酮酸羧化酶基因家族:结构、功能和分子进化”,Plant.Mol.Biol.,21(3):487-502(1993) |
X17313 | fda | 果糖二磷酸醛缩酶 | Von der Osten,C.H.等“谷氨酸棒杆菌fda基因的分子克隆、核苷酸序列和精细结构分析:谷氨酸棒杆菌果糖-1,6-二磷酸醛缩酶与I类和II类醛缩酶的结构比较”,Mol.Microbiol., |
表2(续) | |||
X53993 | dapA | L-2,3-二氢2,6-吡啶二羧酸合成酶(EC 4.2.1.52) | Bonnassie,S.等“谷氨酸棒杆菌dapA基因的核酸序列”,Nucleic Acids Res.,18(21):6421(1990) |
X54223 | AttB相关位点 | Cianciotto,N.等“白喉棒杆菌、溃疡棒杆菌(Corynebacterium ulcerans)和谷氨酸棒杆菌att B相关位点和λ棒状杆菌噬菌体的attP位点之间的DNA序列同源性”,FEMS,Microbiol,Lett.,66:299-302(1990) | |
X54740 | argS;lysA | 精氨酰-tRNA合成酶;二氨基庚二酸脱羧酶 | Marcel,T.等“谷氨酸棒杆菌lysA基因上游区的核苷酸序列和组构”,Mol.Microbiol.,4(11):1819-1830(1990) |
X55994 | trpL;trpE | 推定的前导肽;邻氨基苯甲酸合酶组分I | Heery,D.M.等“谷氨酸棒杆菌trpE基因的核苷酸序列”,Nucleic Acids Res.,18(23):7138(1990) |
X56037 | ThrC | 苏氨酸合酶 | Han,K.S.等“谷氨酸棒杆菌苏氨酸合酶基因的分子结构”,Mol.Microbiol.,4(10):1693-1702(1990) |
X56075 | attB-相关位点 | 附着位点 | Ciancotto,N.等“白喉棒杆菌、溃疡棒杆菌(Corynebacterium ulcerans)和谷氨酸棒杆菌att B相关位点和λ棒状杆菌噬菌体的attP位点之间的DNA序列同源性”,FEMS.Microbiol.Lett.,66:299-302(1990) |
X57226 | lysC-alpha;lysC-beta;asd | 天冬氨酸激酶α亚基;天冬氨酸激酶β亚基;天冬氨酸β半醛脱氢酶 | Kalinowski,J.等“谷氨酸棒杆菌天冬氨酸激酶的遗传和生物化学分析”,Mol.Microbiol.,5(5):1197-1204(1991);Kalinowski,J.等“天冬氨酸激酶基因lysCα和lysCβ重叠并且在谷氨酸棒杆菌中与天冬氨酸β半醛脱氢酶基因asd相邻”,Mol.Gen.Genet.,224(3):317-324(1990) |
表2(续) | |||
X59403 | gap;pgk;tpi | 甘油醛-3-磷酸;磷酸甘油激酶;丙糖磷酸异构酶 | Eikmanns,B.J.“一种编码三种糖酵解酶甘油醛-3-磷酸脱氢酶,3-磷酸甘油酸激酶和丙糖磷酸异构酶的谷氨酸棒杆菌基因簇的鉴定、序列分析和表达”,J.Bacteriol.,174(19):6076-6086(1992) |
X59404 | gdh | 谷氨酸脱氢酶 | Bormann,E.R.等“编码谷氨酸脱氢酶的谷氨酸棒杆菌gdh基因的分子分析”,Mol.Microbiol.,6(3):317-326(1992) |
X60312 | lysI | L-赖氨酸通透酶 | Seep-Feldhaus,A.H.等“参与赖氨酸摄入的谷氨酸棒杆菌lysl基因的分子分析”,Mol.Microbiol.,5(12):2995-3005(1991) |
X66078 | copI | PsI蛋白 | Joliff,G.等“编码谷氨酸棒杆菌两种主要分泌蛋白之一PS1的csp1基因的克隆和核苷酸序列:PS1的推定的N末端区与分枝杆菌属抗原85复合体相似”,Mol.Microbiol.,6(16):2349-2362(1992) |
X66112 | glt | 柠檬酸合酶 | Eikmanns,B.J.等“编码柠檬酸合酶的谷氨酸棒杆菌gltA基因的克隆序列、表达和转录分析”,Microbiol.,140:1817-1828(1994) |
X67737 | dapB | 二氢2,6-吡啶二羧酸还原酶 | |
X69103 | csp2 | 表层蛋白PS2 | Peyret,J.L.等“编码谷氨酸棒杆菌中一种有序表层蛋白PS2的cspB基因的特征鉴定”,Mol.Microbiol.,9(1):97-109(1993) |
X69104 | IS3相关插入元件 | Bonamy,C.等“一种谷氨酸棒杆菌IS3相关插入序列IS1206的鉴定以及种系发生分析”,Mol.Microbiol.,14(3):571-581(1994) | |
X70959 | leuA | 异丙基苹果酸合酶 | Patek,M.等“谷氨酸棒杆菌中的亮氨酸合成:leuA的酶活性、结构以及leuA失活对赖氨酸合成的影响”,Appl.Environ.Microbiol.,60(1):133-140(1994) |
表2(续) | |||
X71489 | icd | 异柠檬酸脱氢酶(NADP+) | Eikmanns,B.J.等“编码异柠檬酸脱氢酶的谷氨酸棒杆菌icd基因的克隆序列分析、表达和失活以及该酶的生化特征鉴定”,J.Bacteriol.,177(3):774-782(1995) |
X72855 | GDHA | 谷氨酸脱氢酶(NADP+) | |
X75083,X70584 | mtrA | 5-甲基色氨酸抗性 | Heery,D.M.等“谷氨酸棒杆菌色氨酸高产菌株的编码5-甲基色氨酸抗性的序列”,Biochem.Biophys.Res.Commun.,201(3):1255-1262(1994) |
X75085 | recA | Fitzpatrick,R.等“谷氨酸棒杆菌和乳发酵短杆菌的recA突变株的构建和特征鉴定”,Appl.Microbiol.Biotechnol.,42(4):575-580(1994) | |
X75504 | aceA;thiX | 部分异柠檬酸裂合酶;? | Reinscheid,D.J.等“谷氨酸棒杆菌异柠檬酸裂合酶基因的特征鉴定以及该酶的生化分析”,J.Bacteriol.,176(12):3474-3483(1994) |
X76875 | ATP酶β亚基 | Ludwig,W.等“基于延伸因子Tu和ATP合酶β亚基基因比较分析的细菌种系发生关系”,Antonie Van Leeuwenhoek,64:285-305(1993) | |
X77034 | tuf | 延伸因子Tu | Ludwig,W.等“基于延伸因子Tu和ATP合酶β亚基基因比较分析的细菌种系发生关系”,Antonie Van Leeuwenhoek,64:285-305(1993) |
X77384 | recA | Billman-Jacobe,H.“谷氨酸棒杆菌recA基因的核苷酸序列”,DNASeq.,4(6):403-404(1994) | |
X78491 | aceB | 苹果酸合酶 | Reinscheid,D.J.等“编码磷酸转乙酰基酶的谷氨酸棒杆菌pta-ack操纵子的苹果酸合酶:序列分析”,Microbiology,140:3099-3108(1994) |
表2(续) | |||
X80629 | 16S rDNA | 16S核糖体RNA | Rainey,F.A.等“红细菌属(Rhodococcus)和诺卡氏菌属(Norcardia)的种系发生分析和诺卡氏菌来自红细菌辐射的进化起源的证据”,Microbiol.,141:523-528(1995) |
X81191 | gluA;gluB;gluC;gluD | 谷氨酸摄入系统 | Kronemeyer,W.等“编码谷氨酸棒杆菌谷氨酸摄入系统的gluABCD簇的结构”,J.Bacteriol.,77(5):1152-1158(1995) |
X81379 | dapE | 琥珀酰二氨基庚二酸脱琥珀酰基酶 | Wehrmann,A.等“互补大肠杆菌dapE的谷氨酸棒杆菌的不同DNA片段的分析”,Microbiology,40:3349-56(1994) |
X82061 | 16S rDNA | 16S核糖体RNA | Ruimy,R.等“由小亚基核糖体DNA序列分析推导的棒杆菌属的种系发生”,Int.J.Syst.Bacteril.,45(4):740-746(1995) |
X82928 | asd;lysC | 天冬氨酸半醛脱氢酶;? | Serebrijski,I.等“asd基因的多拷贝抑制和proA突变体中由异源proA引起的渗透胁迫依赖性互补作用”,J.Bacteriol.,177(24):7255-7260(1995) |
X82929 | proA | γ-谷氨酰磷酸还原酶 | Serebrijski,I.等“asd基因的多拷贝抑制和proA突变体中由异源proA引起的渗透胁迫依赖性互补作用”,J.Bacteriol.,177(24):7255-7260(1995) |
X84257 | 16S rDNA | 16S核糖RNA | Pascual,C.等“基于16S rRNA基因序列的棒杆菌属的种系发生分析”,Int.J.Syst.Bacteriol.,45(4):724-728(1995) |
X85965 | aroP;dapE | 芳香氨基酸通透酶;? | Wehrmann等“谷氨酸棒杆菌脯氨酸dapE的相邻序列的功能分析说明存在编码芳香族氨基酸转运蛋白的aroP”,J.Bacteriol.,177(20):5991-5993(1995) |
表2(续) | |||
X86157 | argB;argC;argD;argF;argJ | 乙酰谷氨酸激酶;N-乙酰-γ-谷氨酰磷酸还原酶;乙酰鸟氨酸氨基转移酶;鸟氨酸氨甲酰转移酶;谷氨酸N-乙酰基转移酶 | Sakanyan,V.等“谷氨酸棒杆菌中精氨酸生物合成乙酰基循环的基因和酶:精氨酸途径早期步骤中的酶进化”,Microbiology,142:99-108(1996) |
X89084 | pta;ackA | 磷酸乙酰基转移酶;乙酸激酶 | Reinscheid,D.J.等“编码磷酸转乙酰基酶和乙酸激酶的谷氨酸棒杆菌pta-ack操纵子的克隆、序列分析、表达和失活”,Microbiology,145:503-513(1999) |
X89850 | attB | 附着位点 | Le Marrec,C.等“感染“金黄节杆菌(Arthrobacter aureus)C70”的phi AAU2定点整合功能的遗传特征鉴定”,J.Bacteriol.,178(7):1996-2004(1996) |
X90356 | 启动子片段F1 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90357 | 启动子片段F2 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90358 | 启动子片段F10 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90359 | 启动子片段F13 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90360 | 启动子片段F22 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90361 | 启动子片段F34 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) |
表2(续) | |||
X90362 | 启动子片段F37 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90363 | 启动子片段F45 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90364 | 启动子片段F64 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90365 | 启动子片段F75 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90366 | 启动子片段PF101 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90367 | 启动子片段PF104 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X90368 | 启动子片段PF109 | Patek,M.等“谷氨酸棒杆菌启动子:克隆、分子分析和共有基序的搜索”,Microbiology,142:1297-1309(1996) | |
X93513 | amt | 铵转运系统 | Siewe,R.M.等“谷氨酸棒杆菌(甲基)铵摄入载体的功能和遗传特征鉴定”,J.Biol.Chem.,271(10):5398-5403(1996) |
X93514 | betP | 甜菜碱转运系统 | Pater,H.等“编码匹配溶质甜菜碱转运系统的谷氨酸棒杆菌betP基因分离、特征鉴定和表达”,J.Bacteriol.,178(17):5229-5234(1996) |
表2(续) | |||
X95649 | orf4 | Patek,M.等“编码参与L-赖氨酸合成的两种酶的谷氨酸棒杆菌dapB-ORF2-dapA-ORF4操纵子的鉴定和转录分析”,Biotechnol.Lett.,19:1113-1117(1997) | |
X96471 | lysE;lysG | 赖氨酸输出蛋白;赖氨酸输出调节蛋白 | Vrljic,M.等“具有新型细胞功能的新型转运蛋白:谷氨酸棒杆菌的L-赖氨酸输出”,Mol.Microbiol.,22(5):815-826(1996) |
X96580 | panB;panC;xylB | 3-甲基-2-羟基丁酸羟甲基转移酶;泛解酸-β-丙氨酸连接酶;木酮糖激酶 | Sahm,H.等“谷氨酸棒杆菌中的D-泛解酸合成以及将panBC和编码L-缬氨酸合成的基因应用于D-泛解酸的过量生产”,Appl.Environ.Microbiol.,65(5):1973-1979(1999) |
X96962 | 插入序列IS1207和转座酶 | ||
X99289 | 延伸因子P | Ramos,A.等“编码氨基酸生产菌乳发酵短杆菌(谷氨酸棒杆菌ATCC 13869)中延伸因子P的基因的克隆、测序和表达”,Gene,198:217-222(1997) | |
Y00140 | thrB | 高丝氨酸激酶 | Mateos,L.M.等“乳发酵短杆菌的高丝氨酸激酶(thrB)基因的核苷酸序列”,Nucleic Acids Res.,15(9):3922(1987) |
Y00151 | ddh | 中-二氨基庚二酸D-脱氢酶(EC 1.4.1.16) | Ishino,S.等“谷氨酸棒杆菌的中-二氨基庚二酸D-脱氢酶基因的核苷酸序列”,Nucleic Acids Res.,15(9):3917(1987) |
Y00476 | thrA | 高丝氨酸脱氢酶 | Mateos,L.M.等“乳发酵短杆菌的高丝氨酸脱氢酶(thrA)基因的核苷酸序列”,Nucleic Acids Res.,15(24)10598(1987) |
Y00546 | hom;thrB | 高丝氨酸脱氢酶;高丝氨酸激酶 | Peoples,O.P.等“谷氨酸棒杆菌hom-thrB操纵子的核苷酸序列和精细结构分析”,Mol.Microbiol.,2(1):63-72(1988) |
表2(续) | |||
Y08964 | murC;ftsQ/divD;ftsZ | UPD-N-乙酰胞壁酸-丙氨酸连接酶;分裂起始蛋白或细胞分裂蛋白;细胞分裂蛋白 | Honrubia,M.P.等“乳发酵短杆菌ftsZ基因的鉴定、表征和染色体组构”,Mol.Gen.Genet,259(1):97-104(1998) |
Y09163 | putP | 高亲合性脯氨酸转运系统 | Peter,H.等“谷氨酸棒杆菌putP基因的分离和匹配溶质的低亲合性摄入系统的特征鉴定”,Arch.Microbiol.,168(2):143-151(1997) |
Y09548 | pyc | 丙酮酸羧化酶 | Peters-Wendisch,P.G.等“谷氨酸棒杆菌丙酮酸羧化酶:pyc基因的特征鉴定、表达和失活”,Microbiology,144:915-927(1998) |
Y09578 | leuB | 3-异丙基苹果酸脱氢酶 | Patek,M.等“谷氨酸棒杆菌leuB基因的分析”,Appl.Microbiol.Biotechnol.,50(1):42-47(1998) |
Y12472 | 附着位点噬菌体Phi-16 | Moreau,S.等“棒杆菌噬菌体Phi-16的定点整合:整合载体的构建”,Microbiol.,145:539-548(1999) | |
Y12537 | proP | 脯氨酸/ectoine摄入系统蛋白 | Peter,H.等“谷氨酸棒杆菌备有四种匹配溶质的第二载体:脯氨酸/ectoine摄入系统ProP以及ectoine/脯氨酸/甜菜碱载体EctP的鉴定、测序和特征鉴定”,J.Bacteriol.,180(22):6005-6012(1998) |
Y13221 | glnA | 谷氨酰胺合成酶I | Jakoby,M.等“编码谷氨酸合成酶I的谷氨酸棒杆菌glnA基因的分离”,FEMS Microbiol.Lett.,154(1):81-88(1997) |
Y16642 | lpd | 二氢硫辛酰胺脱氢酶 | |
Y18059 | 附着位点棒杆菌噬菌体304L | Moreau,S.等“&phi;304L整合功能的分析:棒杆菌噬菌体中的一种整合酶组件”,Virology,255(1):150-159(1999) |
表2(续) | |||
Z21501 | argS;lysA | 精氨酰-tRNA合成酶;二氨基庚二酸脱羧酶(部分) | Oguiza,J.A.等“编码精氨酰-tRNA合成酶的基因位于乳发酵短杆菌lysA基因的上游区:精氨酸对argS-lysA簇表达的调节”,J.Bacteriol.,175(22):7356-7362(1993) |
Z21502 | dapA;dapb | 二氢2,6-吡啶二羧酸合酶;二氢2,6-吡啶二羧酸还原酶 | Pisabarro,A.等“乳发酵短杆菌三个基因(dapA、orf2和dapB)的基因簇编码二氢2,6-吡啶二羧酸还原酶和一种功能未知的第三种多肽”,J.Bacteriol.,175(9):2743-2749(1993) |
Z29563 | thrC | 苏氨酸合酶 | Malumbres,M.等“编码苏氨酸合酶的thrC基因的分析和表达”,Appl.Environ.Microbiol.,60(7)2209-2219(1994) |
ZA6753 | 16S rDNA | 16S核糖体RNA的基因 | |
ZA9822 | sigA | SigAσ因子 | Oguiza,J.A.等“乳发酵短杆菌中的多个σ因子基因:sigA和sigB的特征鉴定”,J.Bacteriol.,178(2):550-553(1996) |
Z49823 | galE;dtxR | 催化活性UDP-半乳糖4-差向异构酶;白喉毒素调节蛋白 | Oguiza,J.A.等“谷氨酸棒杆菌编码UDP-半乳糖4-差向异构酶的galE基因与dmdR基因转录性偶联”,Gene,177:103-107(1996) |
Z49824 | orfI;sigB | “;SigBσ因子 | Oguiza,J.A.等“乳发酵短杆菌中的多个σ因子基因:sigA和sigB的特征鉴定”,Gene,178(2):550-553(1996) |
Z66534 | 转座酶 | Correia,A.等“存在于乳发酵短杆菌ATCC 13869中的IS样元件的克隆和特征鉴定”,Gene,170(1):91-94(1996) | |
1该基因的序列公布于所指明的参考文献中。然而,本申请的发明人获得的序列明显长于所公布的形式。认为所公布的形式依赖于不正确的起始密码子,因此仅代表真实编码区的片段。 |
表3:可以用于实施本发明的棒杆菌属和短杆菌属菌株
ATCC:美国典型培养物保藏中心(American Type Culture Collection,Rockville,MD,USA)。FERM:日本发酵研究所(Fermentation Research Institute,Chiba,Japan)。NRRL:美国农业研究机构保藏中心(ARS Culture Collection,Northern Regional ResearchLaboratory,Peoria,USA)。CECT:西班牙典型培养物保藏中心(Coleccion Espanola de Cultivos Tipo,Valencia,Spain)。NCIMB:英国国立工业和海洋微生物保藏有限公司(National Collection of Industrial andMarine Bacteria Ltd.,Aberdeen,UK)。CBS:荷兰真菌菌种保藏中心(Centraalbureau voor Schimmelcultures,Baarn,NL)。NCTC:英国国立典型培养物保藏中心(National Collection of Type Cultures,London,UK)。DSMZ:德意志微生物保藏中心(Deutsche Sammlung von Mikroorganismen und Zellkulturen,Braunschweig,Germany)。参考文献见:Sugawara,H.等(1993)World directory of collections of cultures of microorganismsBacteria,fungi and yeasts(4th edn),World federation for culture collections world data center onmicroorganisms,Saimata,Japan。
菌种 | ATCC | FRRM | NRRL | CECT | NCIMB | CBS | NCTC | DSMZ |
产氨短杆菌(Brevibacteriumammoniagenes) | 21054 | |||||||
产氨短杆菌 | 19350 | |||||||
产氨短杆菌 | 19351 | |||||||
产氨短杆菌 | 19352 | |||||||
产氨短杆菌 | 19353 | |||||||
产氨短杆菌 | 19354 | |||||||
产氨短杆菌 | 19355 | |||||||
产氨短杆菌 | 19356 | |||||||
产氨短杆菌 | 21055 | |||||||
产氨短杆菌 | 21077 | |||||||
产氨短杆菌 | 21553 | |||||||
产氨短杆菌 | 21580 | |||||||
产氨短杆菌 | 39101 | |||||||
产氨短杆菌(Brevibacteriumbutanicum) | 21196 | |||||||
产氨短杆菌(Brevibacteriumdivaricatum) | 21792 | P928 | ||||||
深黄短杆菌(Brevibacterium flavum) | 21474 | |||||||
深黄短杆菌 | 21129 | |||||||
深黄短杆菌 | 21518 | |||||||
深黄短杆菌 | B11474 | |||||||
深黄短杆菌 | B11472 | |||||||
深黄短杆菌 | 21127 | |||||||
深黄短杆菌 | 21128 | |||||||
深黄短杆菌 | 21427 | |||||||
深黄短杆菌 | 21475 | |||||||
深黄短杆菌 | 21517 | |||||||
深黄短杆菌 | 21528 | |||||||
深黄短杆菌 | 21529 | |||||||
深黄短杆菌 | B11477 | |||||||
深黄短杆菌 | B11478 | |||||||
深黄短杆菌 | 21127 | |||||||
深黄短杆菌 | B11474 | |||||||
希氏短杆菌(Brevibacterium healii) | 15527 | |||||||
酮戊二酸短杆菌(Brevibacteriumketoglutamicum) | 21004 | |||||||
酮戊二酸短杆菌 | 21089 | |||||||
酮戊二酸短杆菌 | 21914 | |||||||
乳发酵短杆菌 | 70 | |||||||
乳发酵短杆菌 | 74 | |||||||
乳发酵短杆菌 | 77 | |||||||
乳发酵短杆菌 | 21798 | |||||||
乳发酵短杆菌 | 21799 | |||||||
乳发酵短杆菌 | 21800 | |||||||
乳发酵短杆菌 | 21801 | |||||||
乳发酵短杆菌 | B11470 | |||||||
乳发酵短杆菌 | B11471 |
菌种 | ATCC | FRRM | NRRL | CECT | NCIMB | CBS | NCTC | DSMZ |
乳发酵短杆菌 | 21086 | |||||||
乳发酵短杆菌 | 21420 | |||||||
乳发酵短杆菌 | 21086 | |||||||
乳发酵短杆菌 | 31269 | |||||||
扩展短杆菌(Brevibacterium linens) | 9174 | |||||||
扩展短杆菌 | 19391 | |||||||
扩展短杆菌 | 8377 | |||||||
Brevibacteriumparaffinolvticum | 11160 | |||||||
短杆菌(Brevibacterium spec.) | 717.73 | |||||||
短杆菌 | 717.73 | |||||||
短杆菌 | 14604 | |||||||
短杆菌 | 21860 | |||||||
短杆菌 | 21864 | |||||||
短杆菌 | 21865 | |||||||
短杆菌 | 21866 | |||||||
短杆菌 | 19240 | |||||||
嗜乙酰乙酸棒杆菌(Corynebacteriumacetoacidophilum) | 21476 | |||||||
嗜乙酰乙酸棒杆菌 | 13870 | |||||||
嗜醋谷棒杆菌 | B11473 | |||||||
嗜醋谷棒杆菌 | B11475 | |||||||
嗜醋谷棒杆菌 | 15806 | |||||||
嗜醋谷棒杆菌 | 21491 | |||||||
嗜醋谷棒杆菌 | 31270 | |||||||
嗜乙酰棒杆菌(Corynebacterium acetophilum) | B3671 | |||||||
产氨短杆菌 | 6872 | 2399 | ||||||
产氨短杆菌 | 15511 | |||||||
Corynebacterium fujiokense | 21496 | |||||||
谷氨酸棒杆菌 | 14067 | |||||||
谷氨酸棒杆菌 | 39137 | |||||||
谷氨酸棒杆菌 | 21254 | |||||||
谷氨酸棒杆菌 | 21255 | |||||||
谷氨酸棒杆菌 | 31830 | |||||||
谷氨酸棒杆菌 | 13032 | |||||||
谷氨酸棒杆菌 | 14305 | |||||||
谷氨酸棒杆菌 | 15455 | |||||||
谷氨酸棒杆菌 | 13058 | |||||||
谷氨酸棒杆菌 | 13059 | |||||||
谷氨酸棒杆菌 | 13060 | |||||||
谷氨酸棒杆菌 | 21492 | |||||||
谷氨酸棒杆菌 | 21513 | |||||||
谷氨酸棒杆菌 | 21526 | |||||||
谷氨酸棒杆菌 | 21543 | |||||||
谷氨酸棒杆菌 | 13287 | |||||||
谷氨酸棒杆菌 | 21851 | |||||||
谷氨酸棒杆菌 | 21253 | |||||||
谷氨酸棒杆菌 | 21514 | |||||||
谷氨酸棒杆菌 | 21516 |
菌种 | ATCC | FRRM | NRRL | CECT | NCIMB | CBS | NCTC | DSMZ |
谷氨酸棒杆菌 | 21299 | |||||||
谷氨酸棒杆菌 | 21300 | |||||||
谷氨酸棒杆菌 | 39684 | |||||||
谷氨酸棒杆菌 | 21488 | |||||||
谷氨酸棒杆菌 | 21649 | |||||||
谷氨酸棒杆菌 | 21650 | |||||||
谷氨酸棒杆菌 | 19223 | |||||||
谷氨酸棒杆菌 | 13869 | |||||||
谷氨酸棒杆菌 | 21157 | |||||||
谷氨酸棒杆菌 | 21158 | |||||||
谷氨酸棒杆菌 | 21159 | |||||||
谷氨酸棒杆菌 | 21355 | |||||||
谷氨酸棒杆菌 | 31808 | |||||||
谷氨酸棒杆菌 | 21674 | |||||||
谷氨酸棒杆菌 | 21562 | |||||||
谷氨酸棒杆菌 | 21563 | |||||||
谷氨酸棒杆菌 | 21564 | |||||||
谷氨酸棒杆菌 | 21565 | |||||||
谷氨酸棒杆菌 | 21566 | |||||||
谷氨酸棒杆菌 | 21567 | |||||||
谷氨酸棒杆菌 | 21568 | |||||||
谷氨酸棒杆菌 | 21569 | |||||||
谷氨酸棒杆菌 | 21570 | |||||||
谷氨酸棒杆菌 | 21571 | |||||||
谷氨酸棒杆菌 | 21572 | |||||||
谷氨酸棒杆菌 | 21573 | |||||||
谷氨酸棒杆菌 | 21579 | |||||||
谷氨酸棒杆菌 | 19049 | |||||||
谷氨酸棒杆菌 | 19050 | |||||||
谷氨酸棒杆菌 | 19051 | |||||||
谷氨酸棒杆菌 | 19052 | |||||||
谷氨酸棒杆菌 | 19053 | |||||||
谷氨酸棒杆菌 | 19054 | |||||||
谷氨酸棒杆菌 | 19055 | |||||||
谷氨酸棒杆菌 | 19056 | |||||||
谷氨酸棒杆菌 | 19057 | |||||||
谷氨酸棒杆菌 | 19058 | |||||||
谷氨酸棒杆菌 | 19059 | |||||||
谷氨酸棒杆菌 | 19060 | |||||||
谷氨酸棒杆菌 | 19185 | |||||||
谷氨酸棒杆菌 | 13286 | |||||||
谷氨酸棒杆菌 | 21515 | |||||||
谷氨酸棒杆菌 | 21527 | |||||||
谷氨酸棒杆菌 | 21544 | |||||||
谷氨酸棒杆菌 | 21492 | |||||||
谷氨酸棒杆菌 | B8183 | |||||||
谷氨酸棒杆菌 | B8182 | |||||||
谷氨酸棒杆菌 | B12416 | |||||||
谷氨酸棒杆菌 | B12417 | |||||||
谷氨酸棒杆菌 | B12418 | |||||||
谷氨酸棒杆菌 | B11476 | |||||||
谷氨酸棒杆菌 | 21608 |
菌种 | ATCC | FRRM | NRRL | CECT | NCIMB | CBS | NCTC | DSMZ |
百合花棒杆菌(Corynebacterium lilium) | P973 | |||||||
Corynebacterium nitrilophilus | 21419 | 11594 | ||||||
棒杆菌(Corynebacterium spec.) | P4445 | |||||||
棒杆菌 | P4446 | |||||||
棒杆菌 | 31088 | |||||||
棒杆菌 | 31089 | |||||||
棒杆菌 | 31090 | |||||||
棒杆菌 | 31090 | |||||||
棒杆菌 | 31090 | |||||||
棒杆菌 | 15954 | 20145 | ||||||
棒杆菌 | 21857 | |||||||
棒杆菌 | 21862 | |||||||
棒杆菌 | 21863 |
表4:序列比对结果ID# 长度 Genank命中 长度 登记号 Genbank命中的名称 Genbank命中 同源性 录入日期
NT 的来源 %(GAP)rxa00013 996 GB_GSS4:AQ713475 581 AQ713475 HS_5402_B2_A12_T7ARPCI-11男性BAC文库人基因组克隆平板 人(Homo sapiens) 37,148 99-07-13
=978列=24行B,基因组综述序列。
GB_HTG3:AC007420 130583 AC007420 黑腹果蝇染色体2克隆BACR07M10(D630)RPCI-98 07.M.10map 黑腹果蝇(Drosophila melanogaster) 34,568 99-09-20
24A-24D菌株y;cn bw sp,***正在进行测序***,83非顺序片段。
GB_HTG3:AC007420 130583 AC007420 黑腹果蝇染色体2克隆BACR07M10(D630)RPCI-98 07.M.10map 黑腹果蝇 34,568 99-09-20
24A-24D菌株y;cn bw sp,***正在进行测序***,83非顺序片段。rxa00014 903 GB_BA1:MTCY3A2 25830 Z83867 结核分枝杆菌H37Rv完整基因组;节段136/162 结核分枝杆菌 58,140 98-06-17
GB_BA1:MLCB1779 43254 Z98271 麻风分枝杆菌粘粒B1779 麻风分枝杆菌(Mycobacterium 57,589 97-08-08
leprae)
GB_BA1:SAPURCLU 9120 X92429 白黑链霉菌(S.alboniger)napH、put7、pur10、pur6、pur4、pur5和 环圈链霉菌(Streptomyces 55,667 96-02-28
S pur3基因 anulatus)rxa00030 513 GB_EST21:C89713 767 C89713 C89713 Dictyostelium discoideum SS(H.Urushihara)Dictyostelium Dictyostelium discoideum 45,283 98-04-20
discoideum cDNA克隆SSG229,mRNA序列
GB_EST28:AI497294 484 AI497294 fb63g03.yl Zebrafish WashU MPING EST Danio rerio cDNA 5’,类似 Danio rerio 42,991 99-03-11
于SW:AFP4_MYOOC P80961抗冻蛋白LS-12;mRNA序列
GB_EST21:C92167 637 C92167 C92167 Dictyostelium discoideum SS(H.Urushihara)Dictyostelium Dictyostelium discoideum 44,444 99-07-12
discoideum cDNA克隆SSD179,mRNA序列rxa00032 1632 GB_BA2:AF010496 189370 AF010496 荚膜红细菌菌株SB1003,部分基因组 荚膜红细菌(Rhodobacter 39,689 98-05-12
capsulatus)
GB_BA2:AF018073 9810 AF018073 类球红细菌操纵子调节蛋白(smoC)、周质山梨醇结合蛋白(smoE)、 类球红细菌(Rhodobacter 48,045 97-10-22
山梨醇/甘露醇转运内膜蛋白(smoF)、山梨醇/甘露醇转运内膜蛋白 sphaeroides)
(smoG),山梨醇/甘露醇转运ATP结合转运蛋白(smoK),山梨醇脱
氢酶(smoS)、甘露醇脱氢酶(mtlK)和周质甘露醇结合蛋白(smoM)基
因,完整cds。
GB_BA2:AF045245 5930 AF045245 肺炎克雷伯氏菌D-阿拉伯糖醇转运蛋白(dalT)、D-阿拉伯糖醇激酶 肺炎克雷伯氏菌(Klebsiella 38,514 98-07-16
(dalK)、D-阿拉伯糖醇脱氢酶(dalD)和阻抑蛋白(dalR)基因,完整 pneumoniae)
cds。rxa00041 1342 EM_PAT:E11760 6911 E11760 蔗糖酶基因碱基序列 谷氨酸棒杆菌 99,031 97-10-08
(Rel. 52,
Created)
GB_PAT:I26124 6911 I26124 美国专利5556776的序列4 未知 99,031 96-10-07
GB_IN1:LMFL5883 31934 AL117384 Leishmania major Friedlin染色体23粘粒L5883,完整序列 Leishmania major 43,663 99-10-21rxa00042 882 EM_PAT:E11760 6911 E11760 蔗糖酶基因碱基序列 谷氨酸棒杆菌 94,767 97-10-08
(Rel.52,
Created)
GB_PAT:I26124 6911 I26124 美国专利5556776的序列4 未知 94,767 96-10-07
表4(续)
GB_IN1:CEU33051 4899 U33051 Caenorhabditis elegas sur-2mRNA,完整cds。 Caenorhabditis elegana 40,276 96-01-23rxa00043 1287 GB_PAT:I26124 6911 I26124 美国专利5556776的序列4。 未知 97,591 96-10-07
EM_PAT:E11760 6911 E11760 蔗糖酶基因碱基序列。 谷氨酸棒杆菌 97,591 97-10-
08(Rel.52,
Created)
GB_PR3:AC005174 39769 AC005174 人克隆UWGC:g1564a012,7p14-15,完整序列 人 35,879 98-06-24rxa00098 1743 GB_BA1:MSU88433 1928 U88433 包皮垢分枝杆菌磷酸葡糖异构酶基因,完整cds。 包皮垢分枝杆菌(Mycobacterium 62,658 97-04-19
smegmatis)
GB_BA1:SC5A7 40337 AL031107 天蓝色链霉菌粘粒5A7。 天蓝色链霉菌(Streptomyces 37,638 98-07-27
coelicolor)
GB_BA1:MTCY10D7 39800 Z79700 结核分枝杆菌H37Rv完整基因组;节段44/162。 结核分枝杆菌 36,784 98-06-17rxa00148 2334 GB_BA1:MTCY277 38300 Z79701 结核分枝杆菌H37Rv完整基因组;节段65/162。 结核分枝杆菌 67,457 98-06-17
GB_BA1:MSGY456 37316 AD000001 克隆y456的结核分枝杆菌序列。 结核分枝杆菌 40,883 96-12-03
GB_BA1:MSGY175 18106 AD000015 克隆y175的结核分枝杆菌序列。 结核分枝杆菌 67,457 96-12-10rxa00149 1971 GB_BA1:MSGY456 37316 AD000001 克隆y456的结核分枝杆菌序列。 结核分枝杆菌 35,883 96-12-03
GB_BA1:MSGY175 18106 AD000015 克隆y175的结核分枝杆菌序列。 结核分枝杆菌 51,001 96-12-10
GB_BA1:MTCY277 38300 Z79701 结核分枝杆菌H37Rv完整基因组;节段65/162。 结核分枝杆菌 51,001 98-06-17rxa00195 684 GB_BA1:MTCY274 39991 Z74024 结核分枝杆菌H37Rv完整基因组;节段126/162。 结核分枝杆菌 35,735 98-06-19
GB_BA1:MSGB1529C 36985 L78824 麻风分枝杆菌粘粒B1529DNA序列。 麻风分枝杆菌 57,014 96-06-15
S
GB_BA1:MTCY274 39991 Z74024 结核分枝杆菌H37Rv完整基因组;节段126/162。 结核分枝杆菌 41,892 98-06-19rxa00196 738 GB_BA1:MTCY274 39991 Z74024 结核分枝杆菌H37Rv完整基因组;节段126/162。 结核分枝杆菌 41,841 98-06-19
GB_BA1:MTCY274 39991 Z74024 结核分枝杆菌H37Rv完整基因组;节段126/162。 结核分枝杆菌 36,599 98-06-19
GB_RO:RATCBRQ 10752 M55532 大鼠糖类结合受体基因,完整cds。 褐鼠(Rattus norvegicus) 36,212 93-04-27rxa00202 1065 GB_EST11:AA253618 313 AA253618 mw95c 10.r1 Soares小鼠NML小家鼠cDNA克隆IMAGE:678450 小家鼠(Mus musculus) 38,816 97-03-13
5’,mRNA序列。
GB_EST26:AI390284 490 AI390284 mw96a03.71 Soares小鼠NML小家鼠cDNA克隆IMAGE:678508 小家鼠 42,239 99-02-02
5’,类似于TR:O09171甜菜碱高半胱氨酸甲基转移酶;mRNA
序列。
GB_EST26:AI390280 467 AI390280 mw95c10.y1 Soares小鼠NML小家鼠cDNA克隆IMAGE:678450 小家鼠 37,307 99-02-02
5’,mRNA序列。rxa00206 1161 GB_BA1:MLCB637 44882 Z99263 麻风分枝杆菌粘粒B637。 麻风分枝杆菌 58,312 97-09-17
GB_BA1:MTV012 70287 AL021287 结核分枝杆菌H37Rv完整基因组;节段132/162。 结核分枝杆菌 36,632 99-06-23
表4(续)
GB_BA1:SC6E10 23990 AL109661 天蓝色链霉菌粘粒6E10。 天蓝色链霉菌A3(2) 38,616 99-08-05rxa00224 1074 GB_BA1:BJU32230 1769 U32230 大豆慢生根瘤菌电子转移黄素蛋白小亚单位(etfS)和大亚单位 大豆慢生根瘤菌(Bradyrhizobium 48,038 96-05-25
(etfL)基因,完整cds。 japonicum)
GB_BAI:PDEETFAB 2440 L14864 脱氮副球菌电子转移黄素蛋白α和β亚单位基因,完整cds。 脱氮副球菌(Paracoccus 48,351 93-10-27
denitrficans)
GB_HTG3:AC009689 177954 AC009689 人染色体4克隆104_F_7 map 4,低通过序列取样。 人 38,756 99-08-28rxa00225 909 GB_RO:AF060178 2057 AF060178 小家鼠硫酸乙酰肝素2-磺基转移酶(Hs2s1)mRNA,完整cds。 小家鼠 39,506 98-06-18
GB_GSS11:AQ325043 734 AQ325043 mgxb0020J01r CUGI Rice Blast BAC文库Magnaporthe grisea基因组 Magnaporthe grisea 38,333 99-01-08
克隆mgxb0020J01r,基因组综述序列。
GB_EST31:AI676413 551 AI676413 etmEST0167EtH1禽艾美球虫(Eimeria tenella)cDNA克隆etmc074 禽艾美球虫 35,542 99-05-19
5’,mRNA序列。rxa00235 1398 GB_BA1:MTCY10G2 38970 Z92539 结核分枝杆菌H37Rv完整基因组;节段47/162。 结核分枝杆菌 65,759 98-06-17
GB_BA2:AF061753 3721 AF061753 欧洲亚硝化单胞菌CTP合酶(pyrG)基因,部分cds;和烯醇化酶 欧洲亚硝化单胞菌(Nitrosomonas 58,941 98-08-31
(eno)基因,完整cds。 europaea)
GB_BA2:AF086791 37867 AF086791 Zymomonas mobilis菌株ZM4克隆67E10氨基甲酰磷酸合成酶小亚 Zymomonas mobilis 61,239 98-11-04
单位(carA)、氨基甲酰磷酸合成酶大亚单位(carB)、转录延长因子
(greA)、烯醇化酶(eno)、丙酮酸脱氢酶α亚单位(pdhA)、丙酮酸脱
氢酶β亚单位(pdhB)、核糖核酸酶H(rnh)、高丝氨酸激酶同系物、
乙醇脱氢酶II(adhB)和外切核酸酶ABC亚单位A(uvrA)基因,完整
cds;以及未知基因。rxa00246 1158 GB_BA2:AF012550 2690 AF012550 不动杆菌BD413 ComP(comP)基因,完整cds。 不动杆菌BD413(Acinetobacter sp. 53,726 99-09-27
BD413)
GB_PAT:E03856 1506 E03856 编码乙醇脱氢酶的gDNA。 嗜热脂肪芽孢杆菌(Bacillus 51,688 97-09-29
stearothermophilus)
GB_BA1:BACADHT 1688 D90421 嗜热脂肪芽孢杆菌乙醇脱氢酶的adhT基因。 嗜热脂肪芽孢杆菌 51,602 99-02-07rxa00251 831 GB_BA1:MTCY20G9 37218 Z77162 结核分枝杆菌H37Rv完整基因组;节段25/162。 结核分枝杆菌 42,875 98-06-17
GB_BA1:MTV004 69350 AL009198 结核分枝杆菌H37Rv完整基因组;节段144/162。 结核分枝杆菌 40,380 98-06-18
GB_BA1:MTV004 69350 AL009198 结核分枝杆菌H37Rv完整基因组;节段144/162。 结核分枝杆菌 41,789 98-06-18rxa00288 1134 GB_BA2:AF050114 1038 AF050114 假单胞菌W7藻酸裂合酶,完整cds。 假单胞菌属菌(Pseudomonas 49,898 99-03-03
sp.)W7
GB_GSS3:B16984 469 B16984 344A14.TVC CIT978SKA1人基因组克隆A-344A14,基因组综述序 人 39,355 98-06-04
列。
GB_IN2:AF144549 7887 AF144549 白纹伊蚊(Aedes albopictus)核糖体蛋白L34(rp134)基因,完整cds。 白纹伊蚊 36,509 99-06-03rxa00293 1035 GB_ST1:T28483 313 T28483 EST46182人肾脏人cDNA 3’末端,类似于含黄素蛋白的单加氧酶 人 42,997 95-09-06
1(HT:1956),mRNA序列。
表4(续)
GB_PR1:HUMFMOI 2134 M64082 人含黄素蛋白的单加氧酶(FMO1)mRNA,完整cds。 人 37,915 94-11-08
GB_EST32:AI734238 512 AI734238 zb73c05.y5 Soares_胎儿_肺_NbHL 19W人cDNA克隆IMAGE: 人 41,502 99-06-14
309224 5’,类似于gb:M64082二甲基丙氨酸单加氧酶(人);mRNA
序列rxa00296 2967 GB_HTG6:AC011069 168266 AC011069 黑腹果蝇染色体X克隆BACR11H20(D881)RPCI-98 11.H.20map 黑腹果蝇 33,890 99-12-02
12B-12C菌株y;cn bw sp,***正在进行测序***,92非顺序片段
GB_EST15:AA531468 414 AA531468 nj63d12.sl NCI_CGAP_Pr10人cDNA克隆IMAGE:997175,mRNA 人 40,821 97-08-20
序列。
GB_HTG6:AC011069 168266 AC011069 黑腹果蝇染色体X克隆BACR11H20(D881)RPCI-98 11.H.20map 黑腹果蝇 30,963 99-12-02
12B-12C菌株y;cn bw sp,***正在进行测序***,92非顺序片段。rxa00310 558 GB_VI:VMVY16780 186986 Y16780 轻型天花病毒(Variola minor virus)完整基因组。 轻型天花病毒 35,883 99-09-02
GB_VI:VARCG 186103 L22579 重型天花病毒(Variola major virus)(菌株Bangladesh-1975)完整基因 重型天花病毒 34,664 95-01-12
组。
GB_VI:VVCGAA 185578 X69198 天花病毒(Variola virus)DNA完整基因组。 天花病毒 36,000 96-12-13rxa00317 777 GB_HTG3:AC009571 159648 AC009571 人染色体4克隆57_A_22map 4,***正在进行测序***,8非顺序片 人 36,988 99-09-29
段。
GB_HTG3:AC009571 159648 AC009571 人染色体4克隆57_A_22map 4,***正在进行测序***,8非顺序片 人 36,988 99-09-29
段。
GB_PR3:AC005697 174503 AC005697 人染色体17,克隆hRPK.138_P_22,完整序列。 人 36,340 98-10-09rxa00327 507 GB_BA1:LCATPASEB 1514 X64542 干酪乳杆菌ATP酶β亚单位基因。 干酪乳杆菌(Lactobacillus casei) 34,664 92-12-44
GB_BA1:LCATPASEB 1514 X64542 干酪乳杆菌ATP酶β亚单位基因。 干酪乳杆菌 39,308 92-12-11rxa00328 615 GB_BA1:STYPUTPE 1887 L01138 沙门氏菌(S2980)脯氨酸通透酶(putP)基因,5’末端。 沙门氏菌属菌(Salmonella sp.) 39,623 96-05-09
GB_BA1:STYPUTPF 1887 L01139 沙门氏菌(S2983)脯氨酸通透酶(putP)基因,5’末端。 沙门氏菌属菌 39,623 96-05-09
GB_BA1:STYPUTPI 1889 L01142 沙门氏菌(S3015)脯氨酸通透酶(putP)基因,5’未端。 沙门氏菌属菌 42,906 96-05-09rxa00329 1347 GB_PR3:AC004691 141990 AC004691 人7p14-p15的PAC克隆DJ0740D02,完整序列。 人 38,142 98-05-16
GB_PR4:AC004916 129014 AC004916 人克隆DJ0891 L14,完整序列。 人 38,549 99-07-17
GB_PR3:AC004691 141990 AC004691 人7p14-p15的PAC克隆DJ0740D02,完整序列。 人 35,865 98-05-16rxa00340 1269 GB_BA1:MTCY427 38110 Z70692 结核分枝杆菌H37Rv完整基因组;节段99/162。 结核分枝杆菌 38,940 99-06-24
GB_GSS12:AQ412290 238 AQ412290 RPCI-11-195H2.TV RPCI-11人基因组克隆RPCI-11-195H2,基因 人 36,555 99-03-23
组综述序列。
GB_PL2:AF112871 2394 AF112871 Astasia longa小亚单位核糖体RNA基因,完整序列 Astasia longa 36,465 99-06-28rxa00379 307 GB_HTG1:CEY56A3 224746 AL022280 Caenorhabditis elegans染色体III克隆Y56A3,***正在进行测序 Caenorhabditis elegans 35,179 99-09-06
***,非顺序片段。
GB_HTG1:CEY56A3 224746 AL022280 Caenorhabditis elegans染色体III克隆Y56A3,***正在进行测序 Caenorhabditis elegans 35,179 99-09-06
***,非顺序片段。
表4(续)
GB_PR2:HS134O19 86897 AL034555 染色体lp36.11-36.33的克隆134O19的人DNA序列,完整序列。 人 40,604 99-11-23rxa00381 729 GB_GSS4:AQ730532 416 AQ730532 HS_2149_A1_C06_T7C CTT批准的人基因组精子文库D人基因组 人 35,766 99-07-15
克隆平板=2149,列=11,行=E,基因组综述序列。
GB_EST23:AI120939 561 AI120939 ub74f05.r1 Soares小鼠乳腺NMLMG小家鼠cDNA克隆IMAGE: 小家鼠 41,113 98-09-02
1383489 5’,类似于gb:J04046钙调蛋白(人);gb:M19381小鼠钙调
蛋白(小鼠);mRNA序列。
GB_EST23:AI120939 561 AI120939 ub74f05.rl Soares小鼠乳腺NMLMG小家鼠cDNA克隆IMAGE: 小家鼠 41,113 98-09-02
1383489 5’,类似于gb:J04046钙调蛋白(人);gb:M19381小鼠钙调
蛋白(小鼠);mRNA序列。rxa00385 362 GB_EST32:AI726450 565 AI726450 BNLGHi5857六天棉花纤维陆地棉cDNA 5’,类似于(AF015913) 陆地棉(Gossypium hirsutum) 41,152 99-06-11
Skb1Hs[人],mRNA序列。
GB_GSS4:AQ740856 768 AQ740856 HS_2274_A2_A07_T7C CIT批准的人基因组精子文库D人基因组 人 41,360 99-07-16
克隆平板=2274,列=14,行=A,基因组综述序列。
GB_PR1:HSPAIP 1587 X91809 人GAIP蛋白的mRNA。 人 36,792 96-03-29rxa00388 1134 GB_BA1:MTY25D10 40838 Z95558 结核分枝杆菌H37Rv完整基因组;节段28/162。 结核分枝杆菌 51,852 98-06-17
GB_BA1:MSGY224 40051 AD000004 结核分枝杆菌克隆y224的序列。 结核分枝杆菌 51,852 96-12-03
GB_HTG1:AP000471 72466 AP000471 人染色体21克隆B2308H15 map 21q22.3,***正在进行测序***,非 人 36,875 99-09-13
顺序片段。rxa0427 909 GB_BA1:MSGY126 37164 AD000012 结核分枝杆菌克隆y126的序列。 结核分枝杆菌 60,022 96-12-10
GB_BA1:MTY13D12 37085 Z80343 结核分枝杆菌H37Rv完整基因组;节段156/162。 结核分枝杆菌 60,022 98-06-17
GB_HTG1:CEY48C3 270193 Z92855 Caenorhabditis elegans染色体II克隆Y48C3,***正在进行测序***, Caenorhabditis elegans 28,013 99-05-29
非顺序片段。rxa00483 1587 GB_PR2:HSAF001550 173882 AF001550 人染色体16BAC克隆CIT987SK-334D11完整序列。 人 38,226 97-08-22
GB_BA1:LLCPJW565 12828 Y12736 乳酸乳球菌乳脂亚种质粒pJW565 DNA、abiiM、abiiR基因和orfX 乳酸乳球菌乳脂亚种(Lactococcus 37,492 99-03-01
lactis subsp.cremoris)
GB_HTG2:AC006754 206217 AC006754 Caenorhabditis elegans克隆Y40B10,***正在进行测序***,5个非 Caenorhabditis elegans 36,648 99-02-23
顺序片段。rxa00511 615 GB_PR3:HSE127C11 38423 Z74581 染色体22q11.2-q未端的粘粒E127C11的人DNA序列含有STS。 人 39,831 99-11-23
GB_PR3:HSE127C11 38423 Z74581 染色体22q11.2-q未端的粘粒E127C11的人DNA序列含有STS。 人 36,409 99-11-23rxa00512 718 GB_BA1:MTCY22G8 22550 Z95585 结核分枝杆菌H37Rv完整基因组;节段49/162。 结核分枝杆菌 56,232 98-06-17
表4(续)
GB_BA1:MSGLTA 1776 X60513 包皮垢分枝杆菌柠檬酸合酶gltA基因。 包皮垢分枝杆菌 56,143 91-09-20
GB_BA2:ECU73857 128824 U73857 大肠杆菌微小染色体6-8。 大肠杆菌 48,563 99-07-14rxa00517 1164 GB_HTG2:AC006911 298804 AC006911 Caenorhabditis elegans克隆Y94H6x,***正在进行测序***,15个 Caenorhabditis elegans 37,889 99-02-24
非顺序片段。
GB_HTG2:AC006911 298804 AC006911 Caenorhabditis elegans克隆Y94H6x,***正在进行测序***,15个 Caenorhabditis elegans 37,889 99-02-24
非顺序片段。
GB_EST29:AI602158 481 AI602158 UI-R-AB0-vy-a-01-0-Ul.s2 U-R-AB0褐鼠cDNA克隆U-R-AB0-vy- 褐鼠 40,833 99-04-21
a-01-0-UI 3’,mRNA序列。rxa00518 320 GB_BA2:ECU73857 128824 U73857 大肠杆菌微小染色体6-8。 大肠杆菌 49,688 99-07-14
GB_BA2:STU51879 8371 U51879 鼠伤寒沙门氏菌丙酸代谢操纵子:RpoN激活蛋白同系物(ptpR)、 鼠伤寒沙门氏菌(Salmonella 50,313 99-08-05
羧基磷酸烯醇丙酮酸磷酸变位酶同系物(prpB)、柠檬酸合酶同系物 typhimurium)
(prpC)、prpD和prpE基因,完整cds。
GB_BA2:AE000140 12498 AE000140 大肠杆菌K-12MG1655,400个完整基因组的选择30个。 大肠杆菌 49,688 98-11-12rxa00606 2378 GB_EST32:AU068253 376 AU068253 AU068253 Rice callus稻cDNA克隆C12658_9A,mRNA序列。 稻(Oryza sativa) 41,333 99-06-07
GB_EST13:AA363046 329 AA363046 EST72922 OvaryII人cDNA 5’未端,mRNA序列。 人 34,347 97-04-21
GB_EST32:AU068253 376 AU068253 AU068253 Rice callus稻cDNA克隆C12658_9A,mRNA序列。 稻 41,899 99-06-07rxa00635 1860 GB_BA1:PAORF1 1440 X13378 淀粉皮假单胞菌ORF1 DNA。 淀粉皮假单胞菌(Pseudomonas 53,912 95-07-14
amyloderamosa)
GB_BA1:PAORF1 1440 X13378 淀粉皮假单胞菌ORF1 DNA。 淀粉皮假单胞菌 54,422 95-07-14rxa00679 1389 GB_PL2:AC010871 80381 AC010871 拟南芥染色体IIIBAC T16O11基因组序列,完整序列。 拟南芥(Arabidopsis thaliana) 38,244 99-11-13
GB_PL1:AT81KBGEN 81493 X98130 拟南芥81kb基因组序列。 拟南芥 36,091 97-03-12
GB_PL2:AC010871 80381 AC010871 拟南芥染色体IIIBAC T16O11基因组序列,完整序列。 拟南芥 37,135 99-11-13rxa00680 441 GB_PR3:AC004058 38400 AC004058 人染色体4克隆B241P19map 4q25,完整序列。 人 36,165 98-09-30
GB_PL1:AT81KBGEN 81493 X98130 拟南芥81kb基因组序列。 拟南芥 38,732 97-03-12
GB_PL1:AB026648 43481 AB026648 拟南芥基因组DNA,染色体3,P1克隆:MLJ15,完整序列。 拟南芥 38,732 99-05-07rxa00682 2022 GB_HTG3:AC010325 197110 AC010325 人染色体19克隆克隆CTTB-E1_2568A17,***正在进行测序***,40 人 37,976 99-09-15
个非顺序片段。
GB_HTG3:AC010325 197110 AC010325 人染色体19克隆克隆CITN-E1_2568A17,***正在进行测序***,40 人 37,976 99-09-15
个非顺序片段。
GB_PR4:AC008179 181745 AC008179 人克隆NH0576F01,完整序列。 人 37,143 99-09-28
表4(续)rxa00683 1215 GB_BA2:AE000896 10707 AE000896 热自养甲烷杆菌完整基因组的碱基1189349-1200055(102/148部分 热自养甲烷杆菌 38,429 97-11-15
(section 102/148))。 (Methanobacterium
thermoautotrnphicum)
GB_IN1:DMBR7A4 212734 AL109630 黑腹果蝇克隆BACR7A4。 黑腹果蝇 36,454 99-07-30
GB_EST35:AV163010 273 AV163010 AV163010小家鼠头C57BL/6J 13天胚胎小家鼠cDNA克隆 小家鼠 41,758 99-07-08
3110006J22,mRNA序列。rxa00686 927 GB_HTG2:HSDJ137K 190223 AL049820 人染色体6克隆RP1-137K2map q25.1-25.3,***正在进行测序***, 人 38,031 99-12-03
2 非顺序片段。
GB_HTG2:HSDJ137K 190223 AL049820 人染色体6克隆RP1-137K2map q25.1-25.3,***正在进行测序***, 人 38,031 99-12-03
2 非顺序片段。
GB_EST12:AA284399 431 AA284399 zs57b04.r1 NCI_CGAP_GCB1人cDNA克隆IMAGE:701551 5’, 人 39,205 97-08-14
mRNA序列。rxa00700 927 GB_EST34:AI785570 454 AI785570 uj44d03.x1 Sugano小鼠肝脏mlia小家鼠cDNA克隆IMAGE: 小家鼠 41,943 99-07-02
1922789 3’,类似于gb:Z28407 60S核糖体蛋白L8(人);mRNA
序列。
GB_EST25:AI256147 684 AI256147 ui95e12.x1 Sugano小鼠肝脏mlia小家鼠cDNA克隆IMAGE: 小家鼠 40,791 98-11-12
1890190 3’,类似于gb:Z28407 60S核糖体蛋白L8(人);mRNA
序列。
GB_BA1:CARCG12 2079 X14979 橙色绿屈挠菌反应中心基因1和2。 橙色绿屈挠菌(Chloroflexus 37,721 91-04-23
aurantiacus)rxa00703 2409 GB_BA1:SC7H2 42655 AL109732 天蓝色链霉菌粘粒7H2。 天蓝色链霉菌A3(2) 56,646 99-08-02
GB_BA1:MTCY274 39991 Z74024 结核分枝杆菌H37Rv完整基因组;节段126/162。 结核分枝杆菌 37,369 98-06-19
GB_BA2:REU60056 2520 U60056 Ralstonia eutropha甲酸脱氢酶样蛋白(cbbBc)基因,完整cds。 Ralstonia eutropha 51,087 96-10-16rxa00705 1038 GB_GSS15:AQ604477 505 AQ604477 HS_2116_B1_G07_MR CIT批准的人基因组精子文库D人基因组 人 39,617 99-06-10
克隆平板=2116,列=13,行=N,基因组综述序列。
GB_EST11:AA224340 443 AA224340 zr14e07.s1 Stratagene hNT神经元(#937233)人cDNA克隆IMAGE: 人 35,129 98-03-11
648804 3’,mRNA序列。
GB_EST5:N30648 291 N30648 yw77b02.s1 Soares_胎盘_8-9周_2NbHP 8-9周人cDNA克隆 人 43,986 96-01-05
IMAGE:258219 3’,mRNA序列。rxa00782 1005 GB_BA1:MTCY10D7 39800 Z79700 结核分枝杆菌H37Rv完整基因组;节段44/162。 结核分枝杆菌 63,327 98-06-17
GB_BA1:MLCL373 37304 AL035500 麻风分枝杆菌粘粒L373。 麻风分枝杆菌 62,300 99-08-27
GB_BA2:AF128399 2842 AF128399 铜绿假单胞菌琥珀酰CoA合成酶β亚单位(sucC)和琥珀酰CoA合成 铜绿假单胞菌(Pseudomonas 53,698 99-03-25
酶α亚单位(sucD)基因,完整cds。 aeruginosa)rxa00783 1395 GB_HTG2:AC008158 118792 AC008158 人染色体17克隆hRPK.42_F_20map 17,***正在进行测序***,14 人 35,135 99-07-28
个非顺序片段。
GB_HTG2:AC008158 118792 AC008158 人染色体17克隆hRPK.42_F_20map 17,***正在进行测序***,14 人 35,135 99-07-28
个非顺序片段。
GB_PR3:AC005017 137176 AC005017 人7p14-p15的BAC克隆GS214N13,完整序列。 人 35,864 98-08-08rxa00794 1128 GB_BA1:MTV017 67200 AL021897 结核分枝杆菌H37Rv完整基因组;节段48/162。 结核分枝杆菌 40,331 99-06-24
表4(续)
GB_BA1:MLCB1222 34714 AL049491 麻风分枝杆菌粘粒B1222。 麻风分枝杆菌 61,170 99-08-27
GB_PR2:HS151B14 128942 Z82188 染色体22的克隆151B14的人DNA序列含有生长激素3型受体 人 37,455 99-06-16
(SS3R)基因、类似于核糖体蛋白的L39的假基因、RAC2(RAS相
关的C3肉毒杆菌毒素底物2(P21-RAC2))基因EST、STS、GSS和
CpG岛,完整序列。rxa00799 1767 GB_PL2:AF016327 616 AF016327 Hordeum vulgare Barperml(perml)mRNA,部分cds。 Hordeum vulgare 41,311 97-10-01
GB_HTG2:HSDJ319M 128208 AL079341 人染色体6克隆RP1-319M7 map p21.1-21.3,***正在进行测序 人 36,845 99-11-30
7 ***,非顺序片段。
GB_HTG2:HSDJ319M 128208 AL079341 人染色体6克隆RP1-319M7 map p21.1-21.3,***正在进行测序 人 36,845 99-11-30
7 ***,非顺序片段。rxa00800 1227 GB_BA1:MTV022 13025 AL021925 结核分枝杆菌H37Rv完整基因组;节段100/162。 结核分枝杆菌 63,101 98-06-17
GB_BA1:AB019513 4417 AB019513 天蓝色链霉菌乙醇脱氢酶基因和ABC转运蛋白,完整cds。 天蓝色链霉菌 41,312 98-11-13
GB_PL1:SCSFAARP 7008 X68020 酿酒酵母SFA和ARP基因。 酿酒酵母(Saccharomyces 36,288 94-11-29
cerevisiae)rxa00825 1056 GB_BA1:MTY15C10 33050 Z95436 结核分枝杆菌H37Rv完整基因组;节段154/162。 结核分枝杆菌 39,980 98-06-17
GB_BA1:MLCB2548 38916 AL023093 麻风分枝杆菌粘粒B2548。 麻风分枝杆菌 39,435 99-08-27
GB_BA2:AF169031 1141 AF169031 水稻黄单胞菌水稻致病变种推定核苷酸差向异构酶/脱水酶基因, 水稻黄单胞菌水稻致病变种 46,232 99-09-14
部分cds。 (Xanthomonas oryzae pv.oryzae)rxa00871rxa00872 1077 GB_INI:CEF23H12 35564 Z74472 Caenorhabditis elegans粘粒F2312,完整序列。 Caenorhabditis elegaps 34,502 99-10-08
GB_HTG2:AC007263 167390 AC007263 人染色体14克隆BAC 79J20 map 14q31,***正在进行测序***,5 人 35,714 99-05-24
个顺序片段。
GB_HTG2:AC007263 167390 AC007263 人染色体14克隆BAC 79J20 map 14q31,***正在进行测序***,5 人 35,714 99-05-24
个顺序片段。rxa00879 2241 GB_BA1:MTV049 40360 AL022021 结核分枝杆菌H37Rv完整基因组;节段81/162。 结核分枝杆菌 36,981 98-06-19
GB_PL2:CDU236897 1827 AJ236897 Candida dubliniensis ACT1基因,外显子1-2。 Candida dubliniensis 38,716 99-09-01
GB_PL1:CAACT1A 3206 X16377 白假丝酵母肌动蛋白act1基因。 白假丝酵母(Candida albicans) 36,610 93-04-10rxa00909 955 GB_BA2:AF010496 189370 AF010496 荚膜红细菌菌株SB1003,部分基因组。 荚膜红细菌 51,586 98-05-12
GB_BA1:RMPHA 7888 X93358 Rhizobium meliloti pha[A、B、C、D、E、F、G]基因。 苜蓿中华根瘤菌(Sinorhizobium 48,367 99-03-12
meliloti)
GB_EST16:C23528 317 C23528 C23528 Japanese flounder spleen Paralichthys olivaceus cDNA克隆 Paralichthys olivaceus 41,640 99-09-28
HB5(2),mRNA序列。rxa00913 2118 GB_HTG2:AC007734 188267 AC007734 人染色体18克隆hRPK 44_O _1 map 18,***正在进行测序***,18 人 34,457 99-06-05
个非顺序片段。
表4(续)
GB_HTG2:AC007734 188267 AC007734 人染色体18克隆hRPK.44_O_1 map 18,***正在进行测序***,18 人 34,457 99-06-05
个非顺序片段。
GB_EST18:AA709478 406 AA709478 vv34a05.r1 Stratagene小家鼠心脏(#937316)小家鼠cDNA克隆 小家鼠 42,065 97-12-24
IMAGE:1224272 5’,mRNA序列。rxa00945 1095 GB_HTG4:AC010351 220710 AC010351 人染色体5克隆CITB-H1_2022B6,***正在进行测序***,68个非 人 36,448 99-10-31
顺序片段。
GB_HTG4:AC010351 220710 AC010351 人染色体5克隆CITB-H1_2022B6,***正在进行测序***,68个非 人 36,448 99-10-31
顺序片段。
GB_BA1:MTCY05A6 38631 Z96072 结核分枝杆菌H37Rv完整基因组;节段120/162。 结核分枝杆菌 36,218 98-06-17rxa00965rxa00999 1575 GB_PAT:E13660 1916 E13660 编码6-磷酸萄糖酸脱氢酶的gDNA。 谷氨酸棒杆菌 98,349 98-06-24
GB_BA1:MTCY359 36021 Z83859 结核分枝杆菌H37Rv完整基因组;节段84/162。 结核分枝杆菌 38,520 98-06-17
GB_BA1:MLCB1788 39228 AL008609 麻风分枝杆菌粘粒B1788。 麻风分枝杆菌 64,355 99-08-27rxa01015 442 GB_BA1:MTV008 63033 AL021246 结核分枝杆菌H37Rv完整基因组;节段108/162。 结核分枝杆菌 39,860 98-06-17
GB_BA1:MTV008 63033 AL021246 结核分枝杆菌H37Rv完整基因组;节段108/162。 结核分枝杆菌 39,120 98-06-17rxa01025 1119 GB_BA1:SC7A1 32039 AL034447 天蓝色链霉菌粘粒7A1。 天蓝色链霉菌 55,287 98-12-15
GB_BA1:MSGB1723C 38477 L78825 麻风分枝杆菌粘粒B1723 DNA序列。 麻风分枝杆菌 56,847 96-06-15
S
GB_BA1:MLCB637 44882 Z99263 麻风分枝杆菌粘粒B637。 麻风分枝杆菌 56,676 98-09-17rxa01048 1347 GB_BA2:AF017444 3067 AF017444 苜蓿中华根瘤菌NADP依赖性苹果酸酶(tme)基因,完整cds。 苜蓿中华根瘤菌 53,660 97-11-02
GB_BA1:BSUB0013 218470 Z99116 枯草芽孢杆菌完整基因组(13/21部分):2395261-2613730。 枯草芽孢杆菌(Bacillus subtilis) 37,255 97-11-26
GB_VI:HSV2HG52 154746 Z86099 2型单纯疱疹病毒(菌株HG52),完整基因组。 2型单纯疱疹病毒(human 38,081 98-12-04
herpesvirus 2)rxa01049 1605 GB_HTG2:AC002518 131855 AC002518 人染色体X克隆bWXD20,***正在进行测序***,11个非顺序片 人 35,647 97-09-02
段。
GB_HTG2:AC002518 131855 AC002518 人染色体X克隆bWXD20,***正在进行测序***,11个非顺序片 人 35,647 97-09-02
段。
GB_HTG2:AC002518 131855 AC002518 人染色体X克隆bWXD20,***正在进行测序***,11个非顺序片 人 26,180 97-09-02
段。rxa01077 1494 GB_PR3:HSDJ653C5 85237 AL049743 染色体1p21.3-22.3的克隆653C5的人DNA序列含有CA重复序列 人 36,462 99-11-23
(D1S435)、STS和GSS,完整序列。
GB_BA1:ECU29579 72221 U29579 大肠杆菌K-12基因组,约61-62分钟。 大肠杆菌 41,808 95-07-01
GB_BA1:ECU29579 72221 U29579 大肠杆菌K-12基因组,约61-62分钟。 大肠杆菌 36,130 95-07-01rxa01089 873 GB_GSS8:AQ044021 387 AQ044021 CIT-HSP-2318C18.TR CIT-HSP人基因组克隆2318C18,基因组综 人 36,528 98-07-14
述序列。
表4(续)
GB_GSS8:AQ042907 392 AQ042907 CIT-HSP-2318D17.TR CIT-HSP人基因组克隆2318D17,基因组综 人 35,969 98-07-14
述序列。
GB_GSS8:AQ044021 387 AQ044021 CIT-HSP-2318C18.TR CIT-HSP人基因组克隆2318C18,基因组综 人 44,545 98-07-14
述序列。rxa01093 1554 GB_BA1:CORPYK1 2795 L27126 棒杆菌丙酮酸激酶基因,完整cds。 谷氨酸棒杆菌 100,00 94-12-07
GB_BA1:MTCY01B2 35938 Z95554 结核分枝杆菌H37Rv完整基因组;节段72/162。 结核分枝杆菌 63,771 98-06-17
GB_BA1:MIU65430 1439 U65430 胞内分枝杆菌丙酮酸激酶(pykF)基因,完整cds。 胞内分枝杆菌(Mycobacterium 67,061 96-12-23
intracellulare)rxa01099 948 GB_BA2:AF045998 780 AF045998 谷氨酸棒杆菌肌醇一磷酸磷酸酶(impA)基因,完整cds。 谷氨酸棒杆菌 99,615 98-02-19
GB_BA2:AF051846 738 AF051846 谷氨酸棒杆菌磷酸核糖亚胺甲基-5-氨基-1-磷酸核糖-4-咪唑甲酰 谷氨酸棒杆菌 100,000 98-03-12
胺异构酶(hisA)基因,完整cds。
GB_GSS1:FR0005503 619 Z89313 F.rubripes GSS序列,克隆079B16aE8,基因组综述序列。 Fugu rubripes 37,785 97-03-01rxa01111 541 GB_PR3:AC004063 177014 AC004063 人染色体4克隆B3218,完整序列。 人 35,835 98-07-10
GB_PR3:HS1178121 62268 AL109852 染色体X的克隆RP5-1178121的人DNA序列,完整序列。 人 37,873 99-12-01
GB_HTG3:AC009301 163369 AC009301 人克隆NH0062F14,***正在进行测序***,5个非顺序片段。 人 37,240 99-08-13rxa01130 687 GB_HTG3:AC009444 164587 AC009444 人克隆1_O_3,***正在进行测序***,8个非顺序片段。 人 38,416 99-08-22
GB_HTG3:AC009444 164587 AC009444 人克隆1_O_3,***正在进行测序***,8个非顺序片段。 人 38,416 99-08-22
GB_IN1:DMC66A1 34127 AL031227 黑腹果蝇粘粒66A1。 黑腹果蝇 38,416 98-10-05rxa0193 1572 GB_BA1:CGASO19 1452 X76875 谷氨酸棒杆菌(ASO 19)ATP酶β-亚单位基因。 谷氨酸棒杆菌 99,931 94-10-27
EM_PAT:E09634 1452 E09634 黄色短杆菌UncD基因,其基因产物参与。 谷氨酸棒杆菌 99,242 97-10-
07(Rel.52,
Created)
GB_BA1:MLU15186 36241 U15186 麻风分枝杆菌粘粒L471。 麻风分枝杆菌 39,153 95-03-09rxa01194 495 EM_PAT:E09634 1452 E09634 黄色短杆菌UncD基因,其基因产物参与。 谷氨酸棒杆菌 100,000 97-10-
07(Rel.52,
Created)
GB_BA1:CGASO19 1452 X76875 谷氨酸棒杆菌(ASO 19)ATP酶β-亚单位基因。 谷氨酸棒杆菌 100,000 94-10-27
GB_VI:HEPCRE4B 414 X60570 丙型肝炎病毒推定包膜蛋白的基因组RNA(RE4B分离物)。 丙型肝炎病毒 36,769 92-04-05rxa01200
表4(续)rxa01201 1764 GB_BA1:SLATPSYNA 8560 Z22606 S.lividans i蛋白和ATP合酶基因。 Streptomyces lividans 66,269 95-05-01
GB_BA1:MTCY373 35516 Z73419 结核分枝杆菌H37Rv完整基因组:节段57/162。 结核分枝杆菌 65,437 98-06-17
GB_BA1:MLU15186 36241 U15186 麻风分枝杆菌粘粒L417。 麻风分枝杆菌 39,302 95-03-09rxa01202 1098 GB_BA1:SLATPSYNA 8560 Z22606 S.lividans i蛋白和ATP合酶基因。 Streptomyces lividans 57,087 95-05-01
GB_BA1:SLATPSYNA 8560 Z22606 S.lividans i蛋白和ATP合酶基因。 Streptomyces lividans 38,298 95-05-01
GB_BA1:MCSQSSHC 5538 Y09978 荚膜甲基球菌orfx、orfy、orfz、sqs和shc基因。 荚膜甲基球菌(Methylococcus 37,626 98-05-26
capsulatus)rxa01204 933 GB_PL1:AP000423 154478 AP000423 拟南芥叶绿体基因组DNA,完整序列,菌株:Columbia Chloroplast拟南芥 38,395 99-09-15
GB_HTG6:AC009762 164070 AC009762 人克隆RP11-114I16,***正在进行测序***,39个非顺序片段。 人 35,459 99-12-04
GB_HTG6:AC009762 164070 AC009762 人克隆RP11-114I16,***正在进行测序***,39个非顺序片段。 人 36,117 99-12-04rxa01216 1124 GB_BA1:MTCY10G2 38970 Z92539 结核分枝杆菌H37Rv完整基因组;节段47/162。 结核分枝杆菌 39,064 98-06-17
GB_BA2:AF017435 4301 AF017435 扭脱甲基杆菌甲醇氧化基因、glmU样基因、部分cds和orfL2、 扭脱甲基杆菌(Methylobacterium 42,671 98-03-10
orfL1,orfR基因,完整cds。 exorquens)
GB_BA1:CCRFLBDB 4424 M69228 新月柄杆菌鞭毛基因启动子区。 新月柄杆菌(Caulobacter 41,054 93-04-26
A crescentus)rxa01225 1563 GB_BA2:AF058302 25306 AF058302 玫瑰黄链霉菌富伦菌素生物合成基因族,完整序列。 玫瑰黄链霉菌(Streptomyces 36,205 98-06-02
roseofulvus)
GB_HTG3:AC007301 165741 AC007301 黑腹果蝇染色体2克隆BACR04B09(D576)RPCI-98 04.B.9map 黑腹果蝇 39,922 99-08-17
43E12-44F1菌株y;cn bw sp,***正在进行测序***,150个非顺序
片段。
GB_HTG3:AC007301 16541 AC007301 黑腹果蝇染色体2克隆BACR04B09(D576)RPCI-98 04.B.9map 黑腹果蝇 39,922 99-08-17
43E12-44F1菌株y;cn bw sp,***正在进行测序***,150个非顺序
片段。rxa01227 444 GB_BA1:SERFDXA 3869 M61119 红色糖多孢菌铁氧化还原蛋白(fdxA)基因,完整cds。 红色糖多孢菌(Saccharopolyspora 64,908 96-03-13
erythraea)
GB_BA1:MTV005 37840 AL010186 结核分枝杆菌H37Rv完整基因组;节段51/162。 结核分枝杆菌 62,838 98-06-17
GB_BA1:MSGY348 40056 AD000020 结核分枝杆菌克隆y348的序列。 结核分枝杆菌 61,712 96-12-10rxa01242 900 GB_PR3:AC005697 174503 AC005697 人染色体17,克隆hRPK.138_P_22,完整序列。 人 35,373 98-10-09
GB_HTG3:AC010722 160723 AC010722 人克隆NH0122L09,***正在进行测序***,2个非顺序片段。 人 39,863 99-09-25
GB_HTG3:AC010722 160723 AC010722 人克隆NH0122L09,***正在进行测序***,2个非顺序片段。 人 39,863 99-09-25
表4(续)rxa01243 1083 GB_GSS10:AQ255057 583 AQ255057 mgxb0008N0 1r CUHG1 Rice Blast BAC文库Magnaporthe grisea基因组 Magnaporthe grisea 38,722 98-10-23
克隆mgxb0008N01r基因组综述序列。
GB_IN1:CEK05D4 19000 Z92804 Caenorhabditis elegans粘粒K05D4,完整序列。 Caenorhabditis elegans 35,448 98-11-23
GB_IN1:CEK05D4 19000 Z92804 Caenorhabditis elegans粘粒K05D4,完整序列。 Caenorhabditis elegans 35,694 98-11-23rxa01259 981 GB_BA1:CGLPD 1800 Y16642 谷氨酸棒杆菌1pd基因,完整CDS。 谷氨酸棒杆菌 100,000 99-02-01
GB_HTG4:AC010567 143287 AC010567 黑腹果蝇染色体3L/69C1克隆RPCI98-1 1N6,***正在进行测序 黑腹果蝇 37,178 99-10-16
***,70个非顺序片段。
GB_HTG4:AC010567 143287 AC010567 黑腹果蝇染色体3L/69C1克隆RPCI98-1 1N6,***正在进行测序 黑腹果蝇 37.178 99-10-16
***,70个非顺序片段。rxa01262 1284 GB_BA2:AF172324 14263 AF172324 大肠杆菌GalF(galF)基因,部分cds;O-抗原重复单位转运蛋白 大肠杆菌 59,719 99-10-29
Wzx(wzx)、WbnA(wbnA)、O-抗原聚合酶Wzy(wzy)、WbnB
(wbnB)、WbnC(wbnC)、WbnD(wbnD),WbnE(wbnE)UDP-Glc-4-
差向异构酶GalE(galE)、6-磷酸葡糖酸脱氢酶Gnd(gnd)、UDP-
Glc-6-脱氢酶Ugd(ugd)和WbnF(wbnF)基因,完整cds;以及链长
度决定蛋白Wzz(wzz)基因,部分cds。
GB_BA2:ECU78086 4759 U78086 大肠杆菌假定尿苷-5’-二磷酸葡萄糖脱氢酶(ugd)和O-链长度调节 大肠杆菌 59,735 97-11-05
蛋白(wzz)基因,完整cds。
GB_BA1:D90841 20226 D90841 大肠杆菌基因组DNA,Kohara克隆#351(451-45.5分钟) 大肠杆菌 37,904 97-03-21rxa01311 870 GB_PR3:AC004103 144368 AC004103 人Xp22BACGS-619J3(基因组系统人BAC文库)完整序列。 人 37,340 98-04-18
GB_HTG3:AC007383 215529 AC007383 人克隆NH0310K15,***正在进行测序***,4个非顺序片段。 人 36,385 99-09-25
GB_HTG3:AC007383 215529 AC007383 人克隆NH0310K15,***正在进行测序***,4个非顺序片段。 人 36,385 99-09-25rxa01312 2142 GB_BA2;AE000487 13889 AE000487 大肠杆菌K-12MG1655完整基因组的377/400部分。 大肠杆菌 39,494 98-11-12
GB_BA1:MTV016 53662 AL021841 结核分枝杆菌H37Rv完整基因组;节段143/162。 结核分枝杆菌 46,252 99-06-23
GB_BA1:U00022 36411 U00022 麻风分枝杆菌粘粒L308。 麻风分枝杆菌 46,368 94-03-01rxa01325 795 GB_HTG4:AC009245 215767 AC009245 人染色体7,***正在进行测序***,24个非顺序片段。 人 36,016 99-11-02
GB_HTG4:AC009245 215767 AC009245 人染色体7,***正在进行测序***,24个非顺序片段。 人 36,016 99-11-02
GB_HTG4:AC009245 215767 AC009245 人染色体7,***正在进行测序***,24个非顺序片段。 人 39,618 99-11-02rxa01332 576 GB_HTG6:AC007186 225851 AC007186 黑腹果蝇染色体2克隆BACR03D06(D569)RPCI-98 03.D.6 map 黑腹果蝇 35,366 99-12-07
32A-32A菌株y;cn bw sp,***正在进行测序***,91个非顺序片段。
GB:HTG6:AC007147 202291 AC007147 黑腹果蝇染色体2克隆BACR19N18(D572)RPCI-98 19.N.18map 黑腹果蝇 35,366 99-12-07
32A-32A菌株y;cn bw sp,***正在进行测序***,22个非顺序片段。
表4(续)
GB_HTG3:AC010207 207890 AC010207 人克隆RPCI11-375I20,***正在进行测序***,25个非顺序片段。 人 34,821 99-09-16rxa01350 1107 GB_BA2:AF109682 990 AF109682 北极水螺菌苹果酸脱氢酶(MDH)基因,完整cds。 北极水螺菌(Aquaspirillum 58,487 99-10-19
arcticum)
GB_HTG2:AC006759 103725 AC006759 Caenorhabditis elegans克隆Y40G12,***正在进行测序***,8个非 Caenorhabditis elegans 37,963 99-02-25
顺序片段。
GB_HTG2:AC006759 103725 AC006759 Caenorhabditis elegans克隆Y40G12,***正在进行测序***,8个非 Caenorhabditis elegans 37,963 99-02-25
顺序片段。rxa01365 1497 GB_BA1:MTY20B11 36330 Z95121 结核分枝杆菌H37Rv完整基因组;节段139/162。 结核分枝杆菌 38,011 98-06-17
GB_BA1:XANXANAB 3410 M83231 野油菜黄单胞菌磷酸葡萄糖变位酶和磷酸甘露糖变位酶(xanA)以 野油菜黄单胞菌(Xanthomonas 47,726 93-04-26
及磷酸甘露糖异构酶和GDP-甘露糖焦磷酸化酶(xanB)基因,完整 campestris)
cds。
GB_GSS10:AQ194038 697 AQ194038 RPCI11-47D24.TJ RPCI-11人基因组克隆RPCI-11-47D24,基因组 人 36,599 99-04-20
综述序列。rxa01369 1305 GB_BA1:MTY20B11 36330 Z95121 结核分枝杆菌H37Rv完整基因组;节段139/162。 结核分枝杆菌 36,940 98-06-17
GB_GSS3:B10037 974 B10037 T27A19-T7TAMU拟南芥基因组克隆T27A19,基因组综述序列。 拟南芥 35,284 97-05-14
GB_GSS3:B09549 1097 B09549 T27A19-T7.1 TAMU拟南芥基因组克隆T21A19,基因组综述序 拟南芥 38,324 97-05-14
列。rxa01377 1209 GB_BA1:MTCY71 42729 Z92771 结核分枝杆菌H37Rv完整基因组;节段141/162。 结核分枝杆菌 39,778 99-02-10
GB_HTG5:AC007547 262181 AC007547 人克隆RP11-252O18,WORKING DRAFT序列,121个非顺序片 人 32,658 99-11-16
段。
GB_HTG5:AC007547 262181 AC007547 人克隆RP11-252O18,WORKING DRAFT序列,121个非顺序片 人 38,395 99-11-16
段。rxa01392 1200 GB_BA2:AF072709 8366 AF072709 Streptomyces lividans扩增元件AUD4:假定转录调节蛋白,假定铁 Streptomyces lividans 55,221 98-07-08
氧化还原蛋白,假定细胞色素P450氧化还原酶和假定氧化还原酶
基因,完整cds;以及未知基因。
GB_BA1:CGLYSEG 2374 X96471 谷氨酸棒杆菌lysE和lysG基因。 谷氨酸棒杆菌 100,000 97-02-24
GB_PR4:AC005906 185952 AC005906 人12p13.3 BAC RPCI11-429A20(Roswell Park癌症研究所人BAC 人 36,756 99-01-30
文库)完整序列。rxa01436 1314 GB_BA1:CGPTAACK 3657 X89084 谷氨酸棒杆菌pta基因和ackA基因。 谷氨酸棒杆菌 100,000 99-03-23
A
GB_BA1:D90861 14839 D90861 大肠杆菌基因组DNA,Kohara克隆#405(52.0-52.3分钟)。 大肠杆菌 53,041 97-05-29
GB_PAT:E02087 1200 E02087 编码大肠杆菌乙酸激酶蛋白的DNA。 大肠杆菌 54,461 97-09-29rxa001468 948 GB_GSS1:HPU60627 280 U60627 幽门螺杆菌feoB样DNA序列,基因组综述序列。 幽门螺杆菌(Helicobacter pylori) 39,286 97-04-09
GB_EST31:AI701691 349 AI701691 we81c04.xl Soare_NFL_T_GBC_S1人cDNA克隆IMAGE:2347494 人 39,412 99-06-03
3’,类似于gb:L19686_mal巨噬细胞迁移抑制因子(人);mRNA序
列。
表4(续)
GB_EST15:AA480256 389 AA480256 ne31f04.s1 NCI_CGAP_Co3人cDNA克隆IMAGE:898975 3’,类 人 39,574 97-08-14
似于gb:L19686_mal巨噬细胞迁移抑制因子(人);mRNA序列。rxa01478 1959 GB_BA1:SCI51 40745 AL109848 天蓝色链霉菌粘粒151。 天蓝色链霉菌A3(2) 54,141 99-08-16
GB_BA1:SCE36 12581 AL049763 天蓝色链霉菌粘粒E36。 天蓝色链霉菌 38,126 99-05-05
GB_BA1:CGU43535 2531 U43535 谷氨酸棒杆菌多药抗性蛋白(cmr)基因,完整cds。 谷氨酸棒杆菌 41,852 97-04-09rxa01482 1998 GB_BA1:SC6G4 41055 AL031317 天蓝色链霉菌粘粒6G4。 天蓝色链霉菌 62,149 98-08-20
GB_BA1:U00020 36947 U00020 麻风分枝杆菌粘粒B229。 麻风分枝杆菌 38,303 94-03-01
GB_BA1:MTCY77 22255 Z95389 结核分枝杆菌H37Rv完整基因组;节段146/162。 结核分枝杆菌 38,179 98-06-18rxa01534rxa01535 1530 GB_BA1:MLCB1222 34714 AL049491 麻风分枝杆菌粘粒B1222。 麻风分枝杆菌 66,208 99-08-27
GB_BA1:MTV017 67200 AL021897 结核分枝杆菌H37Rv完整基因组;节段48/162。 结核分枝杆菌 38,553 99-06-24
GB_BA1:PAU72494 4368 U72494 铜绿假单胞菌富马酸酶(fumC)和Mn超氧物酶歧化酶(sodA)基因, 铜绿假单胞菌 52,690 96-10-23
完整cds。rxa01550 1635 GB_BA1:D90907 132419 D90907 集胞蓝细菌属菌PCC6803完整基因组,9/27,1056467-1188885。 集胞蓝细菌属菌(Synechocystis 56,487 99-02-07
sp.)
GB_IN2:AF073177 9534 AF073177 黑腹果蝇糖元磷酸化酶(GlyP)基因,完整cds。 黑腹果蝇 55,100 99-07-01
GB_IN2:AF073179 3159 AF073179 黑腹果蝇糖元磷酸化酶(Glpl)mRNA,完整cds。 黑腹果蝇 56,708 99-04-27rxa01562rxa01569 1482 GB_BA1:D78182 7836 D78182 dTDP-鼠李糖合成途径的变异链球菌DNA,完整cds。 变异链球菌(Streptococcus mutans) 44,050 99-02-05
GB_BA2:AF079139 4342 AF079139 委瑞内拉链霉菌pikCD操纵子,完整序列。 委瑞内拉链霉菌(Streptomyces 38,587 98-10-28
venezuelae)
GB_BA2:AF087022 1470 AF087022 委瑞内拉链霉菌细胞色素P450单加氧酶(picK)基因,完整cds。 委瑞内拉链霉菌 38,621 98-10-15rxa01570 978 GB_BA1:MTCY63 38900 Z96800 结核分枝杆菌H37Rv完整基因组;节段16/162。 结核分枝杆菌 59,035 98-06-17
GB_BA2:AF097519 4594 AF097519 肺炎克雷伯氏菌dTDP-D-葡萄糖4,6脱水酶(rmlB)、葡萄糖-1-磷酸 肺炎克雷伯氏菌 59,714 98-11-04
胸苷转移酶(rmlA),dTDP-4-酮-L-鼠李糖还原酶(rmlD)、dTDP-4-酮
-6-脱氧-D-葡萄糖3,5-差向异构酶(rmlC)和鼠李糖基转移酶(wbbL)
基因,完整cds。
表4(续)
GB_BA2:NGOCPSPS 8905 L09189 脑膜炎萘瑟氏球菌dTDP-D-葡萄糖4,6-脱水酶(rfbB)、葡萄糖-1-磷 脑膜炎萘瑟氏球菌(Neiseria 58,384 96-07-30
酸胸苷转移酶(rfbA)和rfbC基因,完整cds和UPD-葡萄糖-4-差向 meningitidis)
异构酶(galE)假基因。rxa01571 723 GB_BA1:AB011413 12070 AB011413 灰色链球菌Orf2、Orf3、Orf4、Orf5、AfsA、Orf8的基因,部分和 灰色链球菌(StrePtomyces griseus) 57,500 98-08-07
完整cds。
GB_BA1:AB011413 12070 AB011413 灰色链球菌Orf2、Orf3、Orf4、Orf5、AfsA、Orf8的基因。部分和 灰色链球菌 35,655 98-08-07
完整cds。rxa01572 615 GB_BA1:AB011413 12070 AB011413 灰色链球菌Orf2、Orf3、Orf4、Orf5、AfsA、Orf8的基因。部分和 灰色链球菌 57,843 98-08-07
完整cds。
GB_BA1:AB011413 12070 AB011413 灰色链球菌Orf2、Orf3、Orf4、Orf5、AfsA、Orf8的基因,部分和 灰色链球菌 38,119 98-08-07
完整cds。rxa01606 2799 GB_VI:CFU72240 4783 U72240 云杉卷叶蛾核型多角体病毒ETM蛋白同系物、79kDa蛋白同系 云杉卷叶蛾核型多角体病毒 37,115 99-01-29
物、15kDa蛋白同系物和GTA蛋白同系物基因,完整cds。 (Choristoneura fumiferana
nucleopolyhedrovirus)
GB_GSS10:AQ213248 408 AQ213248 HS_3249_B1_A02_MR CIT批准的人基因组精子文库D人基因组 人 34,559 98-09-18
克隆平板=3249 列=3 行=B,基因组综述序列。
GB_GSS8:AQ070145 285 AQ070145 HS_3207_B1_H02_MR CIT批准的人基因组精子文库D人基因组 人 40,351 98-08-05
克隆平板=3207 列=3 行=P,基因组综述序列。rxa01626 468 GB_PR4:AF152510 2490 AF152510 人protocadherinγA3短型蛋白(PCDH-γ-A3)可变区序列,完整cds。 人 34,298 99-07-14
GB_PR4:AF152323 4605 AF152323 人protocadherinγA3(PCDH-γ-A3)mRNA,完整cds。 人 34,298 99-07-22
GB_PR4:AF152509 2712 AF152509 人PCDH-γ-A3基因,异常剪接,mRNA序列。 人 34,298 99-07-14rxa01632 1128 GB_HTG4:AC006590 127171 AC006590 黑腹果蝇染色体2克隆BACR13N02(D543)RPCI-9813.N.2map 黑腹果蝇 33,812 99-10-19
36E-36E菌株y;cn bw sp,***正在进行测序***,101个非顺序片
段。
GB_HTG4:AC006590 127171 AC006590 黑腹果蝇染色体2克隆BACR13N02(D543)RPCI-98 13.N.2 map 黑腹果蝇 33,812 99-10-19
36E-36E菌株y;cn bw sp,***正在进行测序***,101个非顺序片
段。
GB_GSS8:B99182 415 B99182 以CIT-HSP-2280I13.TRCIT-HSP人基因组克隆2280I13,基因组综 人 36,111 98-06-26
述序列。rxa01633 1206 GB_BA1:BSUB0009 208780 Z99112 枯草芽孢杆完整基因组(9/21部分):1598421-1807200。 枯草芽孢杆菌 36,591 97-11-26
GB_BA1:BSUB0009 208780 Z99112 枯草芽孢杆完整基因组(9/21部分):1598421-1807200。 枯草芽孢杆菌 34,941 97-11-26
GB_HTG2:AC006247 174368 AC006247 黑腹果蝇染色体2克隆BACR48I10(D505)RPCI-9848.1.10map 黑腹果蝇 37,037 99-08-02
49E6-49F8菌株y;cn bw sp,***正在进行测序***,17个非顺序片
段。
表4(续)rxa001695 1623 GB_BA1:CGA224946 2408 AJ224946 谷氨酸棒杆菌L-苹果酸:醌氧化还原酶DNA。 谷氨酸棒杆菌 100,000 98-08-11
GB_BA1:MTCY24A1 20270 Z95207 结核分枝杆菌H37Rv完整基因组;节段124/162。 结核分枝杆菌 38,626 98-06-17
GB_IN1:DMU15974 2994 U15974 黑腹果蝇驱动蛋白样蛋白(klp68d)mRNA,完整cds。 黑腹果蝇 36,783 95-07-18rxa01702 1155 GB_BA1:CGFDA 3371 X17313 谷氨酸棒杆菌果糖二磷酸醛缩酶fda基因(EC 4.1.2.13) 谷氨酸棒杆菌 99,913 93-09-12
GB_BA1:MTY13E10 35019 Z95324 结核分枝杆菌H37Rv完整基因组;节段18/162。 结核分枝杆菌 38,786 98-06-17
GB_BA1:MLCB4 36310 AL023514 麻风分枝杆菌粘粒B4。 麻风分枝杆菌 38,238 99-08-27rxa01743 901 GB_IN2:CELC27H5 35840 U14635 Caenorhabditis elegans粘粒C27H5。 Caenorhabditis elegans 35,334 95-07-13
GB_EST24:AI167112 579 AI167112 xylem.est.878杨树木质部λZAPII文库Populus balsamifera subsp Populus balsamifera subsp. 39,222 98-12-03
trichocarpa cDNA 5’,mRNA序列。 trichocarpa
GB_GSS9:AQ102635 347 AQ102635 HS_3048_B1_F08_MFCIT批准的人基因组精子文库D人基因组克 人 40,653 98-08-27
隆平板=3048 列=15 行=L,基因组综述序列。rxa01744 1662 GB_BA1:MTCY01B2 35938 Z95554 结核分枝杆菌H37Rv完整基因组;节段72/162。 结核分枝杆菌 36,650 98-06-17
GB_GSS1:AF009226 665 AF009226 结核分枝杆菌细胞色素D氧化酶亚单位I(appC)基因,部分序列, 结核分枝杆菌 63,438 97-07-31
基因组综述序列。
GB_BA1:SCD78 36224 AL034355 天蓝色链霉菌粘粒D78。 天蓝色链霉菌 53,088 98-11-26rxa01745 836 GB_BA1:MTCY190 34150 Z70283 结核分枝杆菌H37Rv完整基因组;节段98/162。 结核分枝杆菌 62,081 98-06-17
GB_BA1:MLCB22 40281 Z98741 麻风分枝杆菌粘粒B22。 麻风分枝杆菌 61,364 97-08-22
GB_BA2:AE000175 15067 AE000175 大肠杆菌K12 MG1655完整基因组的65/400部分。 大肠杆菌 52,323 98-11-12rxa01758 1140 GB_PR3:HS57G9 113872 Z95116 染色体22q12.1的BAC 57G9的人DNA序列含有EST、CA重复序 人 39,209 99-11-23
列,GSS。
GB_PL2:YSCH9666 39057 U10397 酿酒酵母染色体VIII粘粒9666。 酿酒酵母 40,021 97-09-05
GB_PL2:YSCH9986 41664 U00027 酿酒酵母染色体VIII粘粒9986。 酿酒酵母 34,375 97-08-29rxa01814 1785 GB_BA1:ABCCELB 2058 L24077 木醋杆菌磷酸葡萄糖变位酶(celB)基因,完整cds。 木醋杆菌(Acetobacter xylinus) 62,173 94-09-21
GB_BA1:MTCY22D7 31859 Z83866 结核分枝杆菌H37Rv完整基因组;节段133/162。 结核分枝杆菌 39,749 98-06-17
GB_BA1:MTCY22D7 31859 Z83866 结核分枝杆菌H37Rv完整基因组;节段133/162。 结核分枝杆菌 40,034 98-06-17rxa01851 1809 GB_GSS9:AQ142579 529 AQ142579 HS_2222_B1_H03_MR CIT批准的人基因组精子文库D人基因组 人 38,068 98-09-24
克隆平板=2222 列=5 行=P,基因组综述序列。
GB_IN2:AC005889 108924 AC005889 黑腹果蝇,染色体2L,30A3-30A6区,P1克隆DS06958和 黑腹果蝇 36,557 98-10-30
DS03097,完整序列。
GB_GSS1:AG008814 637 AG008814 人基因组DNA,21q区,克隆:B137B7BB68,基因组综述序列。 人 35,316 99-02-07
表4(续)rxa01859 1050 GB_BA2:AF183408 63626 AF183408 铜绿微囊蓝细菌DNA聚合酶IIIβ亚单位(dnaN)基因,部分cds;微 铜绿微囊蓝细菌(Mycrocystis 36,364 99-10-03
囊藻素合成酶基因簇,完整序列;Uma1(uma1)、Uma2(uma2)、 aeruginosa)
Uma3(uma3)、Uma4(uma4)和Uma5(uma5)基因、完整cds以及Uma6
(uma6)基因、部分cds。
GB_HTG5:AC008031 158889 AC008031 布鲁斯氏锥虫染色体II克隆RPCI93-25N14,***正在进行测序 布鲁斯氏锥虫(Trypanosoma 35,334 99-11-15
***,2个非顺序片段。 brucei)
GB_BA2:AF183408 63626 AF183408 铜绿微囊蓝细菌DNA聚合酶IIIβ亚单位(dnaN)基因,部分cds;微 铜绿微囊蓝细菌 36,529 99-10-03
囊藻素合成酶基因簇,完整序列;Uma1(uma1)、Uma2(uma2)、
Uma3(uma3)、Uma4(uma4)和Uma5(uma5)基因,完整cds以及Uma6
(uma6)基因,部分cds。rxa01865 438 GB_BA1:SERFDXA 3869 M61119 红色糖多孢菌铁氧化还原蛋白(fdxA)基因,完整cds。 红色糖多孢菌 59,862 96-03-13
GB_BA1:MTV005 37840 AL010186 结核分枝杆菌H37Rv完整基因组;节段51/162。 结核分枝杆菌 61,949 98-06-17
GB_BA1:M8GY348 40056 AD000020 结核分枝杆菌克隆y348的序列。 结核分枝杆菌 59,908 96-12-10rxa01882 1113 GB_PR1:HUMADRA2 1491 J03853 人肾脏α-2-肾上腺素能受体基因,完整cds。 人 36,899 93-04-27
C
GB_PR4:HSU72648 4850 U72648 人肾脏α2-C4-肾上腺素能受体基因,完整cds。 人 36,899 98-11-23
GB_GSS3:B42200 387 B42200 HS_1055_B1_A03_MR.abi CIT批准的人基因组精子文库C人基因 人 34,805 97-10-18
组克隆平板=CT777 列=5 行=B,基因组综述序列。rxa01884 1913 GB_BA1:MTCY48 35377 Z74020 结核分枝杆菌H37Rv完整基因组;节段69/162。 结核分枝杆菌 37,892 98-06-17
GB_BA1:SCO001206 9184 AJ001206 天蓝色链霉菌A3(2),糖原代谢簇II。 天蓝色链霉菌 40,413 99-03-29
GB_BA1:D90908 122349 D90908 集胞蓝细菌属菌PCC6803完整基因组,10/27,1188886-1311234。 集胞蓝细菌属菌 47,792 99-02-07rxa01886 897 GB_GSS9:AQ116291 572 AQ116291 RPCI11-49P6.TK.1 RPCI-11人基因组克隆RPCI-11-49P6,基因组 人 43,231 99-04-20
综述序列。
GB_BA2:AE001721 17632 AE001721 海栖热袍菌完整基因组的33/136部分。 海栖热袍菌(Thermotoga maritima) 39,306 99-06-02
GB_EST16:AA567090 596 AA567090 GM01044.5prime GM黑腹果蝇卵巢BlueScript黑腹果蝇cDNA克隆 黑腹果蝇 42,807 98-11-28
GM01044 5prime,mRNA序列。rxa01887 1134 GB_HTG6:AC008147 303147 AC008147 人克隆RP3-405110,***正在进行测序***,102个非顺序片段。 人 36,417 99-12-03
GB_HTG6:AC008147 303147 AC008147 人克隆RP3-405J10,***正在进行测序***,102个非顺序片段。 人 37,667 99-12-03
GB_BA2:ALW243431 26953 AJ243431 鲁氏不动杆菌wzc、wzb、wza、weeA、weeB、wceC、wzx、wzy、 鲁氏不动杆菌(Acinetobacter 39,640 99-10-01
weeD、weeE、weeF、weeG、weeH、weel、weeJ、week、glaU、ugd、 lwoffii)
pgi、galE、pgm(部分)和mip(部分)基因(乳化胶生物合成基因1
簇),菌株RAG-1。rxa01888 658 GB_HTG2:AC008197 125235 AC008197 黑腹果蝇染色体3克隆BACR02L12(D753)RPCI-98 02.L.12map 黑腹果蝇 32,969 99-08-02
94B-94C菌株y;cn bw sp,***正在进行测序***,113个非顺序片
段。
表4(续)
GB_HTG2:AC008197 12535 AC008197 黑腹果蝇染色体3克隆BACR02L12(D753)RPCI-98 02.L.12map 黑腹果蝇 32,969 99-08-02
94B-94C菌株y;cn bw sp,***正在进行测序***,113个非顺序片
段。
GB_EST36:A1881527 598 A1881527 606070C09.y1 606-Schidt lab玉蜀黍cDNA和穗组织cDNA文 玉蜀黍(Zea mays) 43,617 99-07-21
库,mRNA序列。rxa01891 887 GB_VI:HIV232971 621 AJ232971 1型人类免疫缺陷病毒C亚型nef基因,患者MP83。 1型人类免疫缺陷病毒 40,040 99-03-05
GB_PL1:AFCHSE 6158 Y09542 烟曲霉chsE基因。 烟曲霉(Aspergillus fumigatus) 37,844 97-04-01
GB_PR3:AF064858 193387 AF064858 人染色体21q22.3BAC 28F9,完整序列。 人 37,136 98-06-02rxa01895 1051 GB_BA1:CGL238250 1593 AJ238250 谷氨酸棒杆菌ndh基因。 谷氨酸棒杆菌 100,000 99-04-24
GB_BA2:AF038423 1376 AF038423 包皮垢分枝杆菌NADH脱氢酶(ndh)基因,完整cds。 包皮垢分枝杆菌 65,254 98-05-05
GB_BA1:MTCY359 36021 Z83859 结核分枝杆菌H37Rv完整基因组:节段84/162。 结核分枝杆菌 40,058 98-06-17rxa01901 1383 GB_BA1:MSG38COS 37114 L01095 麻风分枝杆菌基因组DNA序列,粘粒B35 bfr基因,完整cds。 麻风分枝杆菌 59,551 94-09-06
GB_BA1:SCE63 37200 AL035640 天蓝色链霉菌粘粒E63。 天蓝色链霉菌 39,468 99-03-17
GB_PR3:AF093117 147216 AF093117 人染色体7qtelo BAC E3,完整序列。 人 39,291 98-10-02rxa01927 1503 GB_BA1:CGPAN 2164 X96580 谷氨酸棒杆菌panB、panC & xylB基因。 谷氨酸棒杆菌 38,384 99-05-11
GB_BA1:ASXYLA 1905 X59466 节杆菌属菌N.R.R.L.B3728 D-木糖(D-葡萄糖)异构酶的xylA基因 节杆菌属菌(Arthrobacter sp.) 56,283 92-05-04
GB_HTG3:AC009500 176060 AC009500 人克隆NH0511A20,***正在进行测序***,6个非顺序片段。 人 37,593 99-08-24rxa01952 1836 GB_BA2:AE000739 13335 AE000739 Aquifex aeolicus完整基因组的71/109部分。 Aquifex aeolicus 36,309 98-03-25
GB_EST28:AI519629 612 AI519629 LD39282.5prime LD黑腹果蝇胚胎pOT2黑腹果蝇cDNA克隆 黑腹果蝇 41,941 99-03-16
LD39282 5prime,mRNA序列。
GB_EST21:AA949396 767 AA949396 LD28277.5prime LD黑腹果蝇胚胎pOT2黑腹果蝇cDNA克隆 黑腹果蝇 39,855 98-11-25
LD28277 5prime,mRNA序列。Rxa01989 630 GB_BA1:BSPGIA 1822 X16639 嗜热脂肪芽孢杆菌磷酸葡萄糖异构酶A的pgiA基因(EC 5.3.1.9)。 嗜热脂肪芽孢杆菌 66,292 95-04-20
GB_BA1:BSUB0017 217420 Z99120 枯草芽孢杆菌完整基因组(17/21部分):3197001-3414420。 枯草芽孢杆菌 37,255 97-11-26
GB_BA2:AF132127 8452 AF132127 变异链球菌山犁醇磷酸烯醇丙酮酸:糖磷酸转移酶操纵子、完整 变异链球菌 63,607 99-09-28
序列和未知基因。rxa02026 720 GB_BAI:SXSCRBA 3161 X67744 木糖葡萄球菌scrB和scrR基因。 木糖葡萄球菌(Staphylococcus 67,778 96-11-28
xylosus)
GB_BA1:BSUB0020 212150 Z99123 枯草芽孢杆菌完整基因组(20/21部分):3798401-4010550。 枯草芽孢杆菌 35,574 97-11-26
GB_BA1:BSGENR 97015 X73124 枯草芽孢杆菌基因组区段(325-333) 枯草芽孢杆菌 51,826 93-11-02rxa02028 526 GB_BA1:MTCI237 27030 Z94752 结核分枝杆菌H37Rv完整基因组;节段46/162。 结核分枝杆菌 54,476 98-06-17
表4(续)
GB_PL2:SCE9537 66030 U18778 酿酒酵母染色体V粘粒9537、9581、9495、9867和λ克隆5898。 酿酒酵母 36,100 97-08-01
GB_GSS13:AQ501177 767 AQ501177 V26G9mTn-3xHA/lacZ插入文库酿酒酵母基因组5’,基因组综述 酿酒酵母 32,039 99-04-29
序列。rxa02054 1140 GB_BA1:MLCB1222 34714 AL049491 麻风分枝杆菌粘粒B1222。 麻风分枝杆菌 61,896 99-08-27
GB_BA1:MTY13E12 43401 Z95390 结核分枝杆菌H37Rv完整基因组;节段147/162。 结核分枝杆菌 59,964 98-06-17
GB_BA1:MTU43540 3453 U43540 结核分枝杆菌rfbA、鼠李糖生物合成蛋白(rfbA)和rmlC基因,完 结核分枝杆菌 59,659 97-08-14
整cds。rxa02056 2891 GB_PAT:E14601 4394 E14601 乳发酵短杆菌α-酮基谷氨酸脱氢酶。 谷氨酸棒杆菌 98,928 99-07-28
GB_BA1:D84102 4394 D84102 谷氨酸棒杆菌2-氧代谷氨酸脱氢酶DNA,完整cds。 谷氨酸棒杆菌 98,928 99-02-06
GB_BA1:MTV006 22440 AL021006 结核分枝杆菌H37Rv完整基因组;节段54/162。 结核分枝杆菌 39,265 98-06-18rxa02061 1617 GB_HTG7:AC005883 211682 AC005883 人染色体17克隆RP11-958E11 map 17,***正在进行测序***,2个 人 37,453 99-12-08
顺序片段。
GB_PL2:ATAC003033 84254 AC003033 拟南芥染色体IIBAC T21L14基因组序列,完整序列。 拟南芥 37,711 97-12-19
GB_PL2:ATAC002334 75050 AC002334 拟南芥染色体IIBAC F25I18基因组序列,完整序列。 拟南芥 37,711 98-03-04rxa02063 1350 GB_BA1:SCGLGC 1518 X89733 天蓝色链霉菌glgC基因的DNA。 天蓝色链霉菌 56,972 99-07-12
GB_GSS4:AQ687350 786 AQ687350 nbxb0074H11r CUGI稻BAC文库稻基因组克隆nbxb0074H11r,基 稻 40,696 99-07-01
因组综述序列。
GB_EST38:AW028530 444 AW02853 wv27f10.x1 NCI_CGAP_Kid11人cDNA克隆IMAGE:2530795 3’, 人 36,795 99-10-27
0 类似于WP:T03G11.6CE04874;mRNA序列。rxa02100 2348 GB_BA1:MSGY151 37036 AD000018 结核分枝杆菌克隆y151的序列。 结核分枝杆菌 40,156 96-12-10
GB_BA1:MTCY130 32514 Z73902 结核分枝杆菌H37Rv完整基因组;节段59/162。 结核分枝杆菌 55,218 98-06-17
GB_BA1:SCO001205 9589 AJ001205 天蓝色链霉菌A3(2)糖原代谢簇I。 天蓝色链霉菌 38,475 99-03-29rxa02122 822 GB_BA1:D90858 13548 D90858 大肠杆菌基因组DNA,Kohara克隆#401(51.3-51.6分钟) 大肠杆菌 38,586 97-05-29
GB_EST37:AI948595 469 AI948595 wq07d12.x1 NCI_CGAP_Kid12人cDNA克隆IMAGE:2470583 3’; 人 37,259 99-09-06
mRNA序列。
GB_HTG3:AC010387 220665 AC010387 人染色体5克隆CITB-H1_2074D8,***正在进行测序***,77个非 人 38,868 99-09-15
顺序片段。rxa02140 1200 GB_BA1:MSGB1551C 36548 L78813 麻风分枝杆菌粘粒B1551 DNA序列。 麻风分枝杆菌 51,399 96-06-15
S
GB_BA1:MSGB1554C 36548 L78814 麻风分枝杆菌粘粒B1554 DNA序列。 麻风分枝杆菌 51,399 96-06-15
S
GB_RO:AF093099 2482 AF093099 小家鼠转录因子TBLYM(Tblym)mRNA,完整cds。 小家鼠 36,683 99-10-01rxa02142 774 GB_BA1:MTCY190 34150 Z70283 结核分枝杆菌H37Rv完整基因组;节段98/162。 结核分枝杆菌 57,292 98-06-17
表4(续)
GB_BA1:SC6G10 36734 AL049497 天蓝色链霉菌粘粒6G10。 天蓝色链霉菌 35,058 99-03-24
GB_BA1:AB016787 5550 AB016787 恶臭假单胞菌细胞色素o泛醌醇氧化酶A-E的基因和2ORF,完 恶臭假单胞菌(Pseudomonas 47,403 99-08-05
整cds。 putida)rxa02143 1011 GB_BA1:MTCY190 34150 Z70283 结核分枝杆菌H37Rv完整基因组;节段98/162。 结核分枝杆菌 57,317 98-06-17
GB_BA1:MSGB1551C 36548 L78813 麻风分枝杆菌粘粒B1551DNA序列。 麻风分枝杆菌 38,159 96-06-15
S
GB_BA1:MSGB1554C 36548 L78814 麻风分枝杆菌粘粒B1554DNA序列。 麻风分枝杆菌 38,159 96-06-15
Srxa02144 1347 GB_BA1:MTCY190 34150 Z70283 结核分枝杆菌H37Rv完整基因组;节段98/162。 结核分枝杆菌 55,530 98-06-17
GB_HTG3:AC011500_ 300851 AC011500 人染色体19克隆CIT978SKB_60E11,***正在进行测序***,246 人 39,659 00-02-18
0 个非顺序片段。
GB_HTG3:AC011500_ 300851 AC011500 人染色体19克隆CIT978SKB_60E11,***正在进行测序***,246 人 39,659 00-02-18
0 个非顺序片段。rxa02147 1140 GB_EST28:AI492095 485 AI492095 tg07a01.x1 NCI_CGAP_CLL1人cDNA克隆IMAGE:2108040 3’; 人 39,798 99-03-30
mRNA序列。
GB_EST10:AA15767 376 AA157467 zo50e01.r1 Stratagene内皮细胞937223人cDNA克隆IMAGE: 人 36,436 96-12-11
590328 5’;mRNA序列。
GB_EST10:AA15767 376 AA157467 zo50e01.r1 Stratagene内皮细胞937223人cDNA克隆IMAGE: 人 36,436 96-12-11
590328 5’;mRNA序列。rxa02149 1092 GB_PR3:HSBK277P6 61698 AL117347 染色体lq25.3-31.2的克隆277P6的人DNA序列,完整序列。 人 36,872 99-11-23
GB_BA2:EMB065R07 360 AF116423 Rhizobium etli突变体MB045 RosR-转录调节序列。 Rhizobium etli 43,175 99-12-06
5
GB_EST34:AI789323 574 AI789323 uk53g05.y1 Sugano小鼠肾脏mkia小家鼠cDNA克隆IMAGE: 小家鼠 39,715 99-07-02
1972760 5’,类似于WP:K11H12.8CE12160;mRNA序列。rxa02175 1416 GB_BA1:CGGLTG 3013 X66112 谷氨酸棒杆菌柠檬酸合酶glt基因和ORF。 谷氨酸棒杆菌 100,000 95-02-17
GB_BA1:MTCY31 37630 Z73101 结核分枝杆菌H37Rv完整基因组;节段41/162。 结核分枝杆菌 64,331 98-06-17
GB_BA1:MLCB57 38029 Z99494 麻风分枝杆菌粘粒B57。 麻风分枝杆菌 62,491 99-02-10rxa02196 816 GB_RO:RATDAPRP 2819 M76426 褐鼠二肽基氨基肽酶相关蛋白(dpp6)mRNA,完整cds。 褐鼠 38,791 95-05-31
GB_GSS8:AQ012162 763 AQ012162 127PB037070197染色体II粘粒文库类球红细菌基因组克隆 类球红细菌 40,044 98-06-04
127PB037070197,基因组综述序列。
GB_RO:RATDAPRP 2819 M76426 褐鼠二肽基氨基肽酶相关蛋白(dpp6)mRNA,完整cds。 褐鼠 37,312 95-05-31rxa02209 1694 GB_BA1:AB025424 2995 AB025424 谷氨酸棒杆菌顺乌头酸酶基因,部分cds。 谷氨酸棒杆菌 99,173 99-04-03
GB_BA2:AF002133 15437 AF002133 鸟分枝杆菌菌株GIR10转录调节蛋白(mav81)基因、部分cds、顺 鸟分枝杆菌(Mycobacterium 40,219 98-03-26
乌头酸酶(acn),侵袭素1(inv1)、侵袭素2(inv2)、转录调节蛋白 avium)
(moxR),酮脂酰基还原酶(fabG)、烯醇还原酶(inhA)和亚铁螯合酶
(mav272)基因,完整cds。
表4(续)
GB_BA1:MTV007 32806 AL021184 结核分枝杆菌H37Rv完整基因组;节段64/162。 结核分枝杆菌 38,253 98-06-17rxa02213 874 GB_BA1:AB025424 2995 AB025424 谷氨酸棒杆菌顺乌头酸酶基因,部分cds。 谷氨酸棒杆菌 99,096 99-04-03
GB_BA1:MTV007 32806 AL021184 结核分枝杆菌H37Rv完整基因组;节段64/162。 结核分枝杆菌 34,937 98-06-17
GB_BA2:AF002133 15437 AF002133 鸟分枝杆菌菌株GIR10转录调节蛋白(mav81)基因、部分cds、顺 鸟分枝杆菌 36,885 98-03-26
乌头酸酶(acn)、侵袭素1(inv1)、侵袭素2(inv2)、转录调节蛋白
(moxR)、酮脂酰基还原酶(fabG)、烯醇还原酶(inhA)和亚铁螯合酶
(mav272)基因,完整cds。rxa02245 780 GB_BA2:RCU23145 5960 U23145 荚膜红细菌卡尔文循环二氧化碳固定操纵子:果糖-1,6-/景天庚酮 荚膜红细菌 48,701 97-10-28
糖-1,7-二磷酸醛缩酶(cbbA)基因、部分cds、II型核酮糖-1,5-二磷酸
羧化酶/加氧酶(cbbM)基因、完整cds和卡尔文循环操纵子:戊糖-
5-磷酸-3-差向异构酶(cbbE)、磷酸甘油酸磷酸酶(cbbZ)和cbbY基
因,完整cds。
GB_BA1:ECU82664 139818 U82664 大肠杆菌9-11分钟基因组序列。 大肠杆菌 39,119 97-01-11
GB_HTG2:AC007922 158858 AC007922 人染色体18克隆hRPK.178_F_10 map 18,***正在进行测序***,11 人 33,118 99-06-26
个顺序片段。rxa02256 1125 GB_BA1:CGGAPPGK 3804 X59403 谷氨酸棒杆菌甘油醛-3-磷酸、磷酸甘油酸激酶和丙糖磷酸异构酶 谷氨酸棒杆菌 99,289 92-10-05
的gap,pgk和tpi基因。
GB_BA1:SCC54 30753 AL035591 天蓝色链霉菌粘粒C54。 天蓝色链霉菌 36,951 99-06-11
GB_BA1:MTCY493 40790 Z95844 结核分枝杆菌H37Rv完整基因组;节段63/162。 结核分枝杆菌 64,196 98-06-19rxa02257 1338 GB_BA1:CGGAPPGK 3804 X59403 谷氨酸棒杆菌甘油醛-3-磷酸、磷酸甘油酸激酶和丙糖磷酸异构酶 谷氨酸棒杆菌 98,873 92-10-05
的gap,pgk和tpi基因。
GB_BA1:MTCY493 40790 Z95844 结核分枝杆菌H37Rv完整基因组;节段63/162。 结核分枝杆菌 61,273 98-06-19
GB_BA2:MAU82749 2530 U82749 鸟分枝杆菌甘油醛-3-磷酸、脱氢酶同系物(gapdh)基因,完整cds; 鸟分枝杆菌 61,772 98-01-06
以及磷酸甘油酸激醇基因,部分cds。rxa02258 900 GB_BA1:CGGAPPGK 3804 X59403 谷氨酸棒杆菌甘油醛-3-磷酸、磷酸甘油酸激酶和丙糖磷酸异构酶 谷氨酸棒杆菌 99,667 92-10-05
的gap,pgk和tpi基因。
GB_BA1:CORPEPC 4885 M25819 谷氨酸棒杆菌磷酸烯醇丙酮酸羧化酶基因。 谷氨酸棒杆菌 100,000 95-12-15
GB_PAT:A09073 4885 A09073 谷氨酸棒杆菌磷酸烯醇丙酮酸羧化酶ppg基因。 谷氨酸棒杆菌 100,000 93-08-25rxa02259 2895 GB_BA1:CORPEPC 4885 M25819 谷氨酸棒杆菌磷酸烯醇丙酮酸羧化酶基因,完整cds。 谷氨酸棒杆菌 100,000 95-12-15
GB_PAT:A09073 4885 A09073 谷氨酸棒杆菌磷酸烯醇丙酮酸羧化酶ppg基因。 谷氨酸棒杆菌 100,000 93-08-25
GB_BA1:CGPPC 3292 X14234 谷氨酸棒杆菌磷酸烯醇丙酮酸羧化酶基因(EC 4.1.1.31)。 谷氨酸棒杆菌 99,827 93-09-12
表4(续)rxa02288 969 GB_PR3:HSDJ94E24 243145 AL050317 染色体20q12的克隆RP1-94E24的人DNA序列,完整序列。 人 36,039 99-12-03
GB_HTG3:AC010091 159526 AC010091 人克隆NH0295A01,***正在进行测序***,4个非顺序片段 人 35,331 99-09-11
GB_HTG3:AC010091 159526 AC010091 人克隆NH0295A01,***正在进行测序***,4个非顺序片段 人 35,331 99-09-11rxa02292 798 GB_BA2:AF125 164 26443 AF125164 脆弱拟杆菌638R多糖B(PS B2)生物合成基因座,完整序列;和 脆弱拟杆菌(Bacteroides fragilis) 39,747 99-12-01
未知基因。
GB_GSS5:AQ744695 827 AQ744695 HS_5505_A2_C06_SP6 RPCI-11男性BAC文库人基因组克隆平板 人 39,185 99-07-16
=1081 行=12 列=E,基因组综述序列。
GB_EST14:AA381925 309 AA381925 EST95058活化T细胞人cDNA 5’未端,mRNA序列。 人 35,922 97-04-21rxa02322 511 GB_BA1:MTCY22G8 22550 Z95585 结核分枝杆菌H37Rv完整基因组;节段49/162。 结核分枝杆菌 57,677 98-06-17
GB_BA1:MTCY22G8 22550 Z95585 结核分枝杆菌H37Rv完整基因组;节段49/162。 结核分枝杆菌 37,143 98-06-17rxa02326 939 GB_BA1:CGPYC 3728 Y09548 谷氧酸棒杆菌pyc基因。 谷氨酸棒杆菌 100,000 98-05-08
GB_BA2:AF038548 3637 AF038548 谷氨酸棒杆菌丙酮酸羧化酶(pyc)基因,完整cds。 谷氨酸棒杆菌 100,000 97-12-24
GB_BA1:MTCY349 43523 Z83018 结核分枝杆菌H37Rv完整基因组;节段131/162。 结核分枝杆菌 37,363 98-06-17rxa02327 1083 GB_BA1:CGPYC 3728 Y09548 谷氨酸棒杆菌pyc基因。 谷氨酸棒杆菌 99,259 98-05-08
GB_BA2:AF03848 3637 AF038548 谷氨酸棒杆菌丙酮酸羧化酶(pyc)基因,完整cds。 谷氨酸棒杆菌 99,259 97-12-24
GB_BA1:MTCY349 43523 Z83018 结核分枝杆菌H37Rv完整基因组;节段131/162。 结核分枝杆菌 41,317 98-06-17rxa02328 1719 GB_BA1:CGPYC 3728 Y09548 谷氨酸棒杆菌pyc基因。 谷氨酸棒杆菌 100,000 98-05-08
GB_BA2:AF038548 3637 AF038548 谷氨酸棒杆菌丙酮酸羧化酶(pyc)基因,完整cds。 谷氨酸棒杆菌 100,000 97-12-24
GB_PL2:AF097728 3916 AF097728 土曲霉丙酮酸羧化酶(Pyc)mRNA,完整cds。 土曲霉(Aspergillus terreus) 52,248 98-10-29rxa02332 1266 GB_BA1:MSGLTA 1776 X60513 包皮垢分枝杆菌柠檬酸合酶gltA基因。 包皮垢分枝杆菌 58,460 91-09-20
GB_BA2:ABU85944 1334 U85944 南极细菌DS2-3R柠檬酸合酶(cisy)基因,完整cds。 南极细菌(Antarctic 57,154 97-09-23
bacterium)DS2-3R
GB_BA2:AE000175 15067 AE000175 大肠杆菌K-12 MG1655完整基因组的65/400部分。 大肠杆菌 38,164 98-11-12rxa02333 1038 GB_BA1:MSGLTA 1776 X60513 包皮垢分枝杆菌柠檬酸合酶gltA基因。 包皮垢分枝杆菌 58,929 91-09-20
GB_PR4:HUAC00229 171681 AC002299 人染色体16BAC克隆CIT987-SKA-113A6~完整基因组序列,完 人 33,070 99-11-23
9 整序列。
表4(续)
GB_HTG2:AC007889 127840 AC007889 黑腹果蝇染色体3克隆BACR48E12(D695)RPCI-98 48.E.12 map 黑腹果蝇 34,897 99-08-02
87A-87B菌株y;cn bw sp,***正在进行测序***,86个非顺序片
段。rxa02399 1467 GB_BA1:CGACEA 2427 X75504 谷氨酸棒杆菌aceA基因和thiX基因(部分)。 谷氨酸棒杆菌 100,000 94-09-09
GB_BA1:CORACEA 1905 L28760 谷氨酸棒杆菌异柠檬酸裂合酶(aceA)基因。 谷氨酸棒杆菌 100,000 95-02-10
GB_PAT:I13693 2135 I13693 美国专利5439822的序列3。 未知 99,795 95-09-26rxa02404 2340 GB_BA1:CGACEB 3024 X78491 谷氨酸棒杆菌(ATCC 13032)aceB基因。 谷氨酸棒杆菌 99,914 95-01-13
GB_BA1:CORACEB 2725 L27123 谷氨酸棒杆菌苹果酸合酶(aceB)基因,完整cds。 谷氨酸棒杆菌 99,786 95-06-08
GB_BA1:PFFC2 5588 Y11998 荧光假单胞菌FC2.1、FC2.2、FC2.3c、FC2.4和FC2.5c读框。 荧光假单胞菌(Pseudomonas 63,539 97-07-11
fluorescens)rxa02414 870 GB_PR4:AC007102 176258 AC007102 人染色体4克隆C0162P16 map 4p16,完整序列。 人 35,069 99-06-02
GB_HTG3:AC011214 183414 AC011214 人克隆5_C_3,低通过序列取样。 人 36,885 99-10-03
GB_HTG3:AC011214 183414 AC011214 人克隆5_C_3,低通过序列取样。 人 36,885 99-10-03rxa02435 681 GB_BA2:AF101055 7457 AF101055 丙酮丁醇梭菌atp操纵子,完整序列。 丙酮丁醇梭菌(Clostridium 39,605 99-03-03
acetobutylicum)
GB_OM:RABPKA 4441 J03247 兔磷酸化酶激酶(α亚单位)mRNA,完整cds。 Oryctolagus cuniculus 36,061 93-04-27
GB_OM:RABPLASIS 4458 M64656 Oryctolagus cuniculus磷酸化酶激酶α亚单位mRNA,完整cds。 Oryctolagus cuniculus 36,000 98-06-22
Mrxa02440 963 GB_EST14:AA417723 374 AA417723 zv01b12.s1 NCI_CGAP_GCB1人cDNA克隆IMAGE:746207 3’, 人 38,770 97-10-16
类似于含有Alu重复元件;含有元件L1重复元件;mRNA序列,
GB_EST11:AA215428 303 AA215428 zr95a07.s1 NCI_CGAP_GCB1人cDNA克隆IMAGE:683412 3’, 人 39,934 97-08-13
类似于含有Alu重复元件;mRNA序列。
GB_BA1:MTCY77 22255 Z95389 结核分枝杆菌H37Rv完整基因组;节段146/162。 结核分枝杆菌 38,889 98-06-18rxa02453 876 GB_EST14:AA426336 375 AA426336 zv53g02.s1 Soares_睾丸_NHT人cDNA克隆IMAGE:757394 3’, 人 38,043 97-10-16
mRNA序列。
GB_BA1:STMAACC8 1353 M55426 弗氏链霉菌氨基葡糖苷乙酰基转移酶(aacC8)基因,完整cds。 弗氏链霉菌(Streptomyces fradiae) 37,097 93-05-05
GB_PR3:AC004500 77538 AC004500 人染色体5,P1克隆1076B9(LBNLH14),完整序列。 人 33,256 98-03-30rxa02474 897 GB_BA1:AB009078 2686 AB009078 解糖短杆菌L-2,3-丁二醇脱氢酶的基因,完整cds。 解糖短杆菌(Brevibacterium 96,990 99-02-13
saccharolyticum)
GB_OM:BTU71200 877 U71200 Bos taurus 3-羟基丁酮还原酶mRNA,完整cds。 Bos taurus 51,659 97-10-08
GB_EST2:F12685 287 F12685 HSC3DA031正常化婴儿脑cDNA人cDNA克隆c-3da03,mRNA序 人 41,509 95-03-14
列。rxa02480 1779 GB_BA1:MTV012 70287 AL021287 结核分枝杆菌H37Rv完整基因组;节段132/162。 结核分枝杆菌 36,737 99-06-23
表4(续)
GB_BA1:SC6G10 36734 AL049497 天蓝色链霉菌粘粒6G10。 天蓝色链霉菌 35,511 99-03-24
GB_BA1:AP000060 347800 AP000060 Aeropyrum pernix基因组碾DNA,3/7部分。 Aeropyrum pernix 48,014 99-06-22rxa02485rxa02492 840 GB_BA1:STMPGM 921 M83661 天蓝色链霉菌磷酸甘油酸变位酶(PGM)基因,完整cds。 天蓝色链霉菌 65,672 93-04-26
GB_BA1:MTCY20G9 37218 Z77162 结核分枝杆菌H37Rv完整基因组;节段25/162。 结核分枝杆菌 61,436 98-06-17
GB_BA1:U00018 42991 U00018 麻风分枝杆菌粘粒B2168。 麻风分枝杆菌 37,893 94-03-01rxa02528 1098 GB_PR2:HS16IN10 56075 AL008707 染色体Xq25的PAC 161N10的人DNA序列,含有EST。 人 37,051 99-11-23
GB_HTG2:AC008235 136017 AC008235 黑腹果蝇染色体3克隆BACR15B19(D995)RPCI-9815B19map 黑腹果蝇 36,822 99-08-02
94F-95A菌株y;cn bw sp,***正在进行测序***,125个非顺序片
段。
GB_HTG2:AC008235 136017 AC008235 黑腹果蝇染色体3克隆BACR15B19(D995)RPCI-9815.B19map 黑腹果蝇 36,822 99-08-02
94F-95A菌株y;cn bw sp,***正在进行测序***,125个非顺序片
段。rxa02539 1641 GB_BA2:RSU17129 17425 U17129 红串红球菌ThcA(thcA)基因,完整cds;以及未知基因。 红串红球菌(Rhodococcus 66,117 99-07-16
erythropolis)
GB_BA1:MTV038 16094 AL021933 结核分枝杆菌H37Rv完整基因组;节段24/162。 结核分枝杆菌 65,174 98-06-17
GB_BA2:AF068264 3152 AF068264 铜绿假单胞菌醌蛋白乙醇脱氢酶(exaA)基因,部分cds;细胞色素 铜绿假单胞菌 65,448 99-03-18
c550前体(exaB),NAD+依赖性乙醛脱氢酶(exaC)和吡略并喹啉醌
合成A(pqqA)基因,完整cds;以及吡略并喹啉醌合成B(pqqB)基
因,部分cds。rxa02551 483 GB_BA1:BACHYPTP 17057 D29985 枯草芽孢杆菌壁结合蛋白和推定蛋白的wapA和orf基因。 枯草芽孢杆菌 53,602 99-02-07
GB_BA1:BACHUTWA 28954 D31856 含hut和wapA基因座的枯草芽孢杆菌基因组。 枯草芽孢杆菌 53,602 99-02-07
PA
GB_BA1:BSGBGLUC 4290 Z34526 枯草芽孢杆菌(Marburg 168)β-葡糖苷通透酶和β-葡糖苷酶的基 枯草芽孢杆菌 53,602 95-07-03rxa02556 1281 GB_HTG3:AC008128 335761 AC008128 人,***正在进行测序***,106个非顺序片段。 人 34,022 99-08-22
GB_HTG3:AC008128 335761 AC008128 人,***正在进行测序***,106个非顺序片段。 人 34,022 99-08-22
GB_PL2:AC005292 99053 AC005292 拟南芥BACF26F24的基因组序列,完整序列。 拟南芥 33,858 99-04-16rxa02560 990 GB_IN1:CEF07A11 35692 Z66511 Caenorhabditis elegans粘粒F07A11,完整序列。 Caenorhabditis elegans 36,420 99-09-02
GB_EST32:AI731605 566 AI731605 BNLGHi10201 6天棉花纤维陆地棉cDNA 5’,类似于(AC004684) 陆地棉 38,095 99-06-11
假定蛋白[拟南芥],mRNA序列。
GB_INI:CEF07A11 35692 Z66511 Caenorhabditis elegans粘粒F07A11,完整序列。 Caenorhabditis elegans 33,707 99-09-02
表4(续)rxa02572 668 GB_BA1:MTCY63 38900 Z96800 结核分枝杆菌H37Rv完整基因组;节段16/162。 结核分枝杆菌 61,677 98-06-17
GB_BA1:MTCY63 38900 Z96800 结核分枝杆菌H37Rv完整基因组;节段16/162。 结核分枝杆菌 37,170 98-06-17
GB_HTG1:HS24H01 46989 AL121632 人染色体21克隆LLNLcl 16H0124 map 21q21,***正在进行测序 人 19,820 99-09-29
***,非顺序片段。rxa02596 1326 GB_BA1:MTV026 23740 AL022076 结核分枝杆菌H37Rv完整基因组;节段157/162, 结核分枝杆菌 36,957 99-06-24
GB_BA2:AF026540 1778 AF026540 结核分枝杆菌UDP-吡喃半乳糖变位酶(glf)基因,完整cds。 结核分枝杆菌 67,627 98-10-30
GB_BA2:MTU96128 1200 U96128 结核分枝杆菌UDP-吡喃半乳糖变位酶(glf)基因,完整cds。 结核分枝杆菌 70,417 98-03-25rxa02611 1775 GB_BA1:MTCY130 32514 Z73902 结核分枝杆菌H37Rv完整基因组;节段59/162。 结核分枝杆菌 38,532 98-06-17
GB_BA1:MSGY151 37036 AD000018 结核分枝杆菌克隆y151的序列。 结核分枝杆菌 60,575 96-12-10
GB_BA1:U00014 36470 U00014 麻风分枝杆菌粘粒B1549。 麻风分枝杆菌 57,486 94-09-29rxa02612 2316 GB_BA1:MTCY130 32514 Z73902 结核分枝杆菌H37Rv完整基因组;节段59/162。 结核分枝杆菌 38,018 98-06-17
GB_BA1:MSGY151 37036 AD000018 结核分枝杆菌克隆y151的序列。 结核分枝杆菌 58,510 96-12-10
GB_BA1:STMGLGEN 2557 L11647 金霉素链霉菌糖原分支酶(glgB)基因,完整cds。 金霉素链霉菌(Streptomyces 57,193 95-05-25
aureofaciens)rxa02621 942 GB_BA1:CGL133719 1839 AJ133719 谷氧酸棒杆菌yjcc基因、amtR基因和citE基因,部分。 谷氨酸棒杆菌 36,858 99-08-12
GB_IN1:CEM106 39973 Z46935 Caenorhabditis elegans粘粒M106,完整序列。 Caenorhabditis elegans 37,608 99-09-02
GB_EST29:AI547662 377 AI547662 U1-R-C3-sz-h-03-0-UI.sl UI-R-C3褐鼠cDNA克隆UI-R-C3-sz-h-03- 褐鼠 50,667 99-07-03
0-UI 3’,mRNA序列。rxa02640 1650 GB_BA1:MTV025 121125 AL022121 结核分枝杆菌H37Rv完整基因组;节段155/162。 结核分枝杆菌 39,187 99-06-24
GB_BA1:PAU49666 4495 U49666 铜绿假单胞菌(orfX)、甘油弥散促进蛋白(glpF)、甘油激酶(glpK)和 铜绿假单胞菌 59,273 97-05-18
Glp阻抑蛋白(glpR)基因,完整cds和(orfK)基因,部分cds。
GB_BA1:AB015974 1641 AB015974 托拉氏假单胞菌甘油激酶的glpK基因,完整cds。 托拉氏假单胞菌(Pseudomonas 58,339 99-08-28
tolaasil)rxa02654 1008 GB_EST6:N65787 512 N65787 20827λ-PRL2拟南芥cDNA克隆232B7T7,mRNA序列。 拟南芥 39,637 98-01-05
GB_PL2:T17H3 65839 AC005916 拟南芥染色体1 BACT17H3序列,完整序列。 拟南芥 33,735 99-08-05
GB_RO:MMU58105 88871 U58105 小家鼠Btk基因座、α-D-半乳糖苷酶(Ags)、核糖体蛋白(L44L)和 小家鼠 35,431 97-02-13
Bruton酪氨酸激酶(Btk)基因,完整cds。rxa02666 891 GB_PR3:AC004643 43411 AC004643 人染色体16,粘粒克隆363E3(LANL),完整序列。 人 38,851 98-05-01
表4(续)
GB_PR3:AC004643 43411 AC004643 人染色体16,粘粒克隆363E3(LANL),完整序列。 人 41,599 98-05-01
GB_BA2:AF049897 9196 AF049897 谷氨酸棒杆菌N-乙酰谷氨酰磷酸还原酶(argC)、鸟氨酸乙酰转移酶 谷氨酸棒杆菌 40,413 98-07-01
(argJ)、N-乙酰谷氨酸激酶(argB)、乙酰鸟氨酸转氨酶(argD)、鸟氨
酸氨基甲酰基转移酶(argF)、精氨酸阻抑蛋白(argR)、精氨琥珀酸
合酶(argG)和精氨琥珀酸裂合酶(argH)基因,完整cds。rxa02675 1980 GB_BA1:PDENQOUR 10425 L02354 脱氮副球菌NADH脱氢酶(URF4)、(NQO8)、(NQO9)、(URF5)、 脱氮副球菌(Paracoccus 40,735 93-05-20
F (URF6)、(NQO10)、(NQO11)、(NQO12)、(NQO13)和(NQO14)基因, denitrificans)
完整cds;生物素[乙酰CoA羧基]连接酶(birA)基因,完整cds。
GB_BA1:MTCY339 42861 Z77163 结核分枝杆菌H37Rv完整基因组;节段101/162。 结核分枝杆菌 36,471 98-06-17
GB_BA1:MXADEVRS 2452 L19029 黄色粘球菌devR和devS基因,完整cds, 黄色粘球菌(Myxococcus xanthus) 38,477 94-01-27rxa02694 1065 GB_BA1:BACLDH 1147 M19394 热溶芽孢杆菌乳酸脱氢酶(LDH)基因,完整cds。 热溶芽孢杆菌(Bacillus 57,371 93-04-26
caldolyticus)
GB_BA1:BACLDHL 1361 M14788 嗜热脂肪芽孢杆菌编码L-乳酸脱氢酶的lct基因,完整cds。 嗜热脂肪芽孢杆菌 57,277 93-04-26
GB_PAT:A06664 1350 AO6664 嗜热脂肪芽孢杆菌lct基因。 嗜热脂肪芽孢杆菌 57,277 93-07-29rxa02729 844 GB_EST15:AA494626 121 AA494626 fa09d04.r1 Zebrafish ICRFzfls Danio rerio cDNA克隆11A22 5’,类似 Danio rerio 50,746 97-06-27
于TR:G1171163 G1171163 G/T错配结合蛋白;mRNA序列。
GB_EST15:AA494626 121 AA494626 fa09d04.r1 Zebrafish ICRFzfls Danio rerio cDNA克隆11A22 5’,类似 Danio rerio 36,364 97-06-27
于TR:G1171163 G1171163 G/T错配结合蛋白;mRNA序列。rxa02730 1161 GB_EST19:AA758660 233 AA758660 ah67d06.s1 Soares_睾丸_NHT人cDNA克隆1320683 3’,mRNA序 人 37,059 98-12-29
列。
GB_EST15:AA494626 121 AA494626 fa09d04.r1 Zebrafish ICRFzfls Danio rerio cDNA克隆11A22 5’,类似 Danio rerio 42,149 97-06-27
于TR:G1171163 G1171163 G/T错配结合蛋白;mRNA序列。
GB_PR4:AC006285 150172 AC006285 人,完整序列。 人 37,655 99-11-15rxa02737 1665 GB_PAT:E13655 2260 E13655 编码葡萄糖-6-磷酸脱氢酶的gDNA。 谷氨酸棒杆菌 99,580 98-06-24
GB_BA1:MTCY493 40790 Z95844 结核分枝杆菌H37Rv完整基因组;节段63/162。 结核分枝杆菌 38,363 98-06-19
GB_BA1:SC5A7 40337 AL031107 天蓝色链霉菌粘粒5A7。 天蓝色链霉菌 39,444 98-07-27rxa02738 1203 GB_PAT:E13655 2260 E13655 编码葡萄糖-6-磷酸脱氢酶的gDNA。 谷氨酸棒杆菌 98,226 98-06-24
GB_BA1:SCC22 22115 AL096839 天蓝色链霉菌粘粒C22。 天蓝色链霉菌 60,399 99-07-12
GB_BA1:SC5A7 40337 AL031107 天蓝色链霉菌粘粒5A7。 天蓝色链霉菌 36,426 98-07-27rxa02739 2223 GB_BA1:AB023377 2572 AB023377 谷氨酸棒杆菌转酮醇酶tkt基因,完整cds。 谷氨酸棒杆菌 99,640 99-02-20
表4(续)
GB_BA1:MLCL536 36224 Z99125 麻风分枝杆菌粘粒L536。 麻风分枝杆菌 61,573 98-12-04
GB_BA1:U00013 35881 U00013 麻风分枝杆菌粘粒B1496。 麻风分枝杆菌 61,573 94-03-01rxa02740 1053 GB_HTG2:AC006247 174368 AC006247 黑腹果蝇染色体2克隆BACR48I10(D505)RPCI-98 48.110map 黑腹果蝇 37,105 99-08-02
49E6-49F8菌株y;cn bw sp,***正在进行测序***,17个非顺序片
段。
GB_HTG2:AC006247 174368 AC006247 黑腹果蝇染色体2克隆BACR48I10(D505)RPCI-98 48.1.10map 黑腹果蝇 37,105 99-08-02
49E6-49F8菌株y;cn bw sp,***正在进行测序***,17个非顺序片
段。
GB_HTG3:AC007150 121474 AC007150 黑腹果蝇染色体2克隆BACR16 P13(D597)RPCO-98 16.P.13map 黑腹果蝇 38,728 99-09-20
49E-49F菌株y;cn bw sp,***正在进行测序***,87个非顺序片段。rxa02741 1089 GB_HTG2:AC004951 129429 AC004951 人克隆DJ1022I14,***正在进行测序***,14个非顺序片段。 人 33,116 98-06-12
GB_HTG2:AC004951 129429 AC004951 人克隆DJ1022I14,***正在进行测序***,14个非顺序片段。 人 33,116 98-06-12
GB_IN1:AB006546 931 AB006546 Ephydatia fluviatilis G蛋白a亚单位4的mRNA,部分cds。 Ephydatia fluviatilis 36,379 99-06-23rxa02743 1161 GB_BA1:MLCL536 36224 Z99125 麻风分枝杆菌粘粒L536。 麻风分枝杆菌 48,401 98-12-04
GB_BA1:U00013 35881 U00013 麻风分枝杆菌粘粒B1496。 麻风分枝杆菌 48,401 94-03-01
GB_HTG2:AC007401 83657 AC007401 人克隆NH0501O07,***正在进行测序***,3个非顺序片段。 人 37,128 99-06-26rxa02797 1026 GB_BA1:CGBETPGE 2339 X93514 谷氨酸棒杆菌betP基因。 谷氨酸棒杆菌 38,889 97-09-08
N
GB_GSS9:AQ148714 405 AQ148714 HS_3136_A1_A03_MR CIT批准的人基因组精子文库D人基因组 人 34,321 98-10-08
克隆平板=3136 列=5 行=A,基因组综述序列。
GB_BA1:BFU64514 3837 U64514 坚强牙孢杆菌dppABC操纵子,二肽转运蛋白dppA基因,部分cds 坚强芽孢杆菌(Bacillus firmus) 38,072 97-02-01
以及二肽转运蛋白dppB和dppC基因,完整cds。rxa02803 680 GB_BA1:U00020 36947 U00020 麻风分枝杆菌粘粒B229 麻风分枝杆菌 34,462 94-03-01
GB_BA2:PSU85643 4032 U85643 丁香假单胞菌丁香致病变种推定二氢叶酸合酶基因,部分cds,调 丁香假单胞菌丁香致病变种 50,445 97-04-09
节蛋白MrsA(mrsA)、丙糖磷酸异构酶(tpiA)、转运蛋白SecG (Pseudomonas syringae pv.syringae)
(secG)、tRNA-Leu、tRNA-Met和15 kDa蛋白基因,完整cds。
GB_BA1:SC6G4 41055 AL031317 天蓝色链霉菌粘粒6G4。 天蓝色链霉菌 59,314 98-08-20rxa02821 363 GB_HTG2:AC008105 91421 AC008105 人染色体17克隆2020_K_17map17,***正在进行测序***,12个 人 37,607 99-07-22
非顺序片段。
GB_HTG2:AC008105 91421 AC008105 人染色体17克隆2020_K_17map17,***正在进行测序***,12个 人 37,607 99-07-22
非顺序片段。
GB_EST33:AV117143 222 AV117143 AV117143小家鼠C57BL/6J 10天胚胎小家鼠cDNA克隆 小家鼠 40,157 99-06-30
26102O0J17,mRNA序列。
表4(续)rxa02829 373 GB_HTG1:HSU9G8 48735 AL008714 人染色体X克隆LL0XNC01-9G8,***正在进行测序***,非顺序片 人 41,595 99-11-23
段。
GB_HTG1:HSU9G8 48735 AL008714 人染色体X克隆LL0XNC01-9G8,***正在进行测序***,非顺序片 人 41,595 99-11-23
段。
GB_PR3:HSU85B5 39550 Z69724 粘粒U85B5的人DNA序列,染色体X标记DXS366和DXS87之 人 41,595 99-11-23
间。rxa03216 1141 GB_HTG3:AC008184 151720 AC008184 黑腹果蝇染色体2克隆BACR04D05(D540)RPCI-98 04.D.5map 黑腹果蝇 39,600 99-08-02
36E5-36F2菌株y;cn bw sp,***正在进行测序***,27个非顺序片
段。
GB_EST15:AA477537 411 AA477537 zu36g 12.r1 Soares卵巢肿瘤NbHOT人cDNA克隆IMAGE:740134 人 37,260 97-11-09
5’,类似于含有Alu重复元件;含有元件HGR重复元件;mRNA
序列。
GB_EST26:AI330662 412 AI330662 fa91d08.y1 zebrafish fin 1天再生Danio rerio cDNA 5’,mRNA序列。 Danio rerio 37,805 98-12-28rxa03215 1038 GB_BA1:SC3F9 19830 AL023862 天蓝色链霉菌粘粒3F9。 天蓝色链霉菌A3(2) 48,657 99-02-10
GB_BA1:SLLINC 36270 X79146 林肯链霉菌(78-11)林可霉素产生基因。 林肯链霉菌(Streptomyces 39,430 96-05-15
lincolnensis)
GB_HTG5:AC009660 204320 AC009660 人染色体15克隆RP11-424J10 map 15,***正在进行测序***,41 人 35,151 99-12-04
个非顺序片段。rxa03224 1288 GB_PR3:AC004076 41322 AC004076 人染色体19,粘粒R30217,完整序列。 人 37,788 98-01-29
GB_PL2:SPAC926 23193 AL110469 栗酒裂殖酵母染色体1粘粒c926。 栗酒裂殖酵母 38,474 99-09-02
(Schizosaccharomyces pombe)
GB_BA2:AE001081 11473 AE001081 Archaeoglobus fulgidus完整基因组26/172部分。 Archaeoglobus fulgidus 35,871 97-12-15实施例部分实施例1:谷氨酸棒杆菌ATCC13032的总基因组DNA的制备
让谷氨酸棒杆菌(ATCC 13032)的培养物于30℃下在BHI培养基(Difco)中振荡生长过夜。通过离心收集细胞,弃去上清液,将细胞重悬于5ml缓冲液I(培养物原始体积的5%--所有指定的体积菌均已经根据100ml培养物体积来计算)。缓冲液I的组成:140.34g/l蔗糖、2.46g/l
×7
、10ml/l
溶液(100g/l,用
调至pH 6.7)、50ml/l M12浓缩物(10g/l
、1g/l
、2/gl
×7
、0.2g/l
、0.5g/l酵母膏(Difco)、10ml/l微量元素混合物(200mg/l
×
、10mg/l
×7
、3mg/l
×4
、30mg/l
、20mg/l
×6
、1mg/l
×6
、3mg/l ×2
、500mg/l络合剂(EDTA或柠檬酸)、100mg/l维生素混合物(0.2mg/l生物素、0.2mg/l叶酸、20mg/l对氨基苯甲酸、20mg/l核黄素、40mg/l泛酸钙(ca-panthothenate)、140mg/l烟酸、40mg/l盐酸吡哆醛、200mg/l肌醇)。将溶菌酶加入上清液中至终浓度2.5mg/ml。于37℃温育约4小时后,细胞壁被降解,通过离心收获所产生的原生质体。沉淀用5ml缓冲液I洗涤1次,用5ml TE缓冲液(10mM
、1mMEDTA,pH8)洗涤1次。将沉淀重悬于4ml TE缓冲液中,加入0.5mlSDS溶液(10%)和0.5ml
溶液(5M)。在加入蛋白酶K至终浓度200μg/ml后,将悬浮夜于37℃温育约18小时。采用标准方法,通过用苯酚、苯酚-氯仿-异戊醇和氯仿-异戊醇抽提,纯化DNA。然后,通过加入1/50体积的3M乙酸钠和2体积乙醇,然后于-20℃温育30分钟,在高速离心机中用SS34转子(Sorvall)以12,000rpm离心30分钟,沉淀所述DNA。将DNA溶于1ml含有20μg/ml RNA酶A的TE缓冲液中,于4℃对1000ml TE缓冲液透析至少3小时。在此期间,更换缓冲液3次。向0.4ml等份的经透析的DNA溶液中,加入0.4ml 2M
和0.8ml乙醇。于-20℃温育30分钟后,通过离心(13,000rpm,Biofuge Fresco,Heraeus,Hanau,德国)收集DNA。DNA沉淀溶于TE缓冲液中。用该方法制备的DNA可以用于所有目的,包括DNA印迹分析或基因组文库的构建。实施例2:构建谷氨酸棒杆菌ATCC13032在大肠杆菌中的基因组文库
采用实施例1所述制备的DNA,按照已知的明确方法(参见例如Sambrook,J.等,(1989)“Molecular Cloning:A Laboratory Manual”,ColdSpring Harbor Laboratory Press,或Ausubel,F.M.等(1994)“CurrentProtocols in Molecular Biology”,John Wiley & Sons.)构建粘粒文库和质粒文库。
可以使用任何质粒或粘粒。具体使用的质粒是质粒pBR322(Sutcliffe,J.G.(1979)Proc.Natl.Acad.Sci.USA,75:3737-3741)、pACYC177(Change & Cohen(1978)J.Bacteriol 134:1141-1156)、pBS质粒系列(pBSSK+、pBSSK-等;Stratagene,LaJolla,USA)或粘粒如SuperCosl(Stratagene,LaJolla,USA)或Lorist6(Gibson,T.J.,RosenthalA.和Waterson,R.H.(1987)Gene 53:283-286)。使用质粒pSL109可构建特别用于谷氨酸棒杆菌的基因文库(Lee,H.-S.和A.J.Sinskey(1994)J.Microbiol.Biotechnol.4:256-263)。实施例3:DNA测序和计算功能分析
应用实施例2描述的基因组文库按照标准方法、尤其是利用ABI377测序仪的链终止法(参见例如Fleischman,R.D.等(1995)“流感嗜血杆菌Rd.的随机全基因组测序和装配”,Science,269:496-512)进行DNA测序。使用具有以下核苷酸序列的测序引物:5’-GGAAACAGTATGACCATG-3’或5’-GTAAAACGACGGCCAGT-3’。实施例4:体内诱变
将质粒(或其它载体)DNA通过保持遗传信息完整性的能力受损的大肠杆菌或其它生物(例如杆菌属杆菌或酵母如酿酒酵母)进行传代,从而可在体内诱变谷氨酸棒杆菌。典型增变株的DNA修复系统基因存在突变(例如muHLS、mutD、mutT等;参考文献见Rupp,W.D.(1996)DNA修复机制,载于:Escherichia coli and Salmonella,第2277-2294页,ASM:Washington.)。这类菌株是本领域技术人员周知的。例如Greener,A.和Callahan,M.(1994)Strategies_7:32-34中介绍了所述菌株的使用。实施例5:大肠杆菌和谷氨酸棒杆菌之间的DNA转移
若干棒杆菌属和短杆菌属物种含有自主复制(综述参见例如Martin,J.F.等(1987)Biotechnology,5:137-146)的内源性质粒(例如pHM1519或pBL1)。采用其中加入谷氨酸棒杆菌复制起点和谷氨酸棒杆菌合适标记的大肠杆菌标准载体(Sambrook,J.等(1989),“MolecularCloning:A Laboratory Manual”,Cold Spring Harbor Laboratory Press或Ausubel,F.M.等(1994)“Current Protocols in Molecular Biology”,JohnWiley & Sons),能够容易地构建大肠杆菌和谷氨酸棒杆菌的穿梭载体。所述复制起点优选取自分离自棒杆菌属和短杆菌属物种的内源性质粒。具体使用的这些物种的转化标记为卡那霉素抗性基因(例如Tn5或Tn903转座子产生的卡那霉素抗性基因)或氯霉素抗性基因(Winnacker,E.L.(1987),From Genes to Clones-Introduction to GeneTechnology,VCH,Weinheim)。所述文献中列举了大量关于构建各种穿梭载体的实例,所述穿梭载体可在大肠杆菌和谷氨酸棒杆菌中复制而且可用于若干目的,包括基因过量表达(参考文献参见例如Yoshihama,M.等(1985)J.Bacteriol.162:591-597,Martin J.F.等(1987)Biotechnology,5:137-146和Eikmanns,B.J.等(1991)Gene,102:93-98)。
应用标准方法可将目的基因克隆入一种上述穿梭载体并将这种杂合载体导入谷氨酸棒杆菌菌株中。可采用以下方法转化谷氨酸棒杆菌:原生质体转化(Kastsumata,R.等(1984)J.Bacteriol.159306-311)、电穿孔(Liebl,E.等(1989)FEMS Microbiol.Letters,53:399-303),如果使用特殊载体,也可以采用接合方法(见例如Sch_fer,A等(1990)J.Bacteriol.172:1663-1666)。通过制备谷氨酸棒杆菌质粒DNA(利用本领域周知的标准方法)并将其转化入大肠杆菌,也可以使穿梭载体从谷氨酸棒杆菌转移到大肠杆菌。采用标准方法可进行这种步骤,但是最好使用Mcr缺陷大肠杆菌菌株如NM522(Gough & Murray(1983)J.Mol.Biol.166:1-19)。
可使用包含pCGl(美国专利号4,617,267)或其片段和任选包含TN903卡那霉素抗性基因(Grindley,N.D.和Joyce,C.M.(1980)Proc.Natl.Acad.Sci.USA 77(12):7176-7180)的质粒在谷氨酸棒杆菌菌株中过量表达各种基因。此外,也可以使用质粒pSL109在谷氨酸棒杆菌菌株中过量表达各种基因(Lee,H.-S.和A.J.Sinskey(1994)J.Microbiol.Biotechnol.4:256-263)。
除了使用复制型质粒之外,也可以通过整合入基因组实现基因过量表达。利用周知的方法可实现在谷氨酸棒杆菌或其它棒杆菌属或短杆菌属物种中的基因组整合,例如与基因组区的同源重组、限制性内切核酸酶介导的融合(REMI)(参见例如DE专利19823834)或应用转座子。也可以采取以下措施修饰调节区(例如启动子、阻抑物和/或增强子)而调节目的基因的活性:利用定点方法(例如同源重组)或基于随机事件的方法(例如转座子诱变或REMI)进行序列修饰、插入或缺失。也可以在本发明一个或多个基因编码区的3’插入转录终止子作用的核酸序列;所述终止子是本领域周知的,例如在Winnacker,E.L.(1987)FromGenes to Clones-Introduction to Gene Technology.VCH:Weinheim)中有其介绍。实施例6:评价突变蛋白的表达
观测转化宿主细胞中的突变蛋白活性依赖于这样的事实:突变蛋白以与野生型蛋白相似的方式和相似的量表达。一种确定突变基因转录水平(可翻译成基因产物的mRNA量的指标)的有用方法是进行RNA印迹(参考文献见例如Ausubel等(1988)Current Protocols in MolecularBiology,Wiley:纽约),其中用可检测标记(通常为放射性或化学发光)标记结合目的基因的设计引物,使得当提取所述生物体培养物的总RNA、在凝胶上电泳、转移至稳定基体上以及与所述探针温育时,所述探针的结合以及结合量可说明存在所述基因以及指示该基因的mRNA量。这样的信息是突变基因转录程度的证据。应用若干本领域周知的方法可从谷氨酸棒杆菌制备总细胞RNA,例如Bormann,E.R.等(1992)Mol.Microbiol.6:317-326介绍的方法。
为了评价所述mRNA翻译蛋白存在与否或相对量,可使用标准技术例如蛋白质印迹(参见例如Ausubel等(1988)Current Protocols inMolecular Biology,Wiley:纽约)。在此过程中,提取总细胞蛋白,经凝胶电泳分离,转移至如硝酸纤维素的基体上以及与特异性结合需要蛋白的探针如抗体温育。这种探针一般用容易检测的化学发光或比色标记物标记。观测到标记物存在和标记物量说明所述细胞中存在需要的突变蛋白及其量。实施例7:遗传改良的谷氨酸棒杆菌的培养-培养基和培养条件
用合成或天然生长培养基培养遗传改良棒杆菌。用于棒杆菌的许多不同生长培养基是众所周知的而且容易获得(Lieb等(1989)Appl.Microbiol.Biotechnol.,32:205-210;von der Osten等(1998)Biotechnology Letters,11:11-16;专利DE 4,120,867;Liebl(1992)“棒杆菌属”,载于:The Procaryotes,第II卷,Balows,A.等编著,Springer-Verlag)。这些培养基的组成是:一种或多种碳源、氮源、无机盐、维生素和微量元素。优选碳源为糖如单糖、二糖或多糖。例如葡萄糖、果糖、甘露糖、半乳糖、核糖、山梨糖、核酮糖、乳糖、麦芽糖、蔗糖、棉子糖、淀粉或纤维素是非常好的碳源。也可以通过复合化合物如糖蜜或精制糖的其它副产品为培养基提供糖。提供不同碳源的混合物也可能是有益的。其它可能的碳源为醇类和有机酸,例如甲醇、乙醇、醋酸或乳酸。氮源通常为有机或无机氮化合物,或者含有这些化合物的物质。典型氮源包括氨气或铵盐,例如氯化铵或硫酸铵、氢氧化铵、硝酸盐、尿素、氨基酸或复合氮源如玉米浆、大豆粉、大豆蛋白、酵母提取物、肉膏等。
培养基可含有的无机盐化合物包括钙、镁、钠、钴、钼、钾、锰、锌、铜和铁的盐酸盐、磷酸盐或硫酸盐。所述培养基中可加入螯合化合物,以保持溶液中的金属离子。特别有效的螯合化合物包括二羟基酚(例如儿茶酚或儿茶酚酸盐)或有机酸(例如柠檬酸)。所述培养基还常规含有其它生长因子,例如维生素或生长促进剂,其实例包括生物素、核黄素、硫胺素、叶酸、烟酸、泛酸和吡哆醇。生长因子和盐通常源自复合培养基组分,例如酵母提取物、糖蜜、玉米浆等。培养基复合物的确切成分与直接实验密切相关而且具体取决于各特定情况。关于培养基优化的信息可在教科书“Applied Microbiol.Physiology,APractical Approach(P.M.Rhodes,P.F.Stanbury编著,IRL Press(1997),第53-73页,ISBN 0 19 963577 3)中获得。也可以从商业供应商选择生长培养基,例如标准1号(Merck)或BHI(grain heart infusion,DIFCO)等。
所有培养基组分通过加热(于1.5帕和121℃ 20分钟)或无菌过滤消毒。所述组分可一起消毒,如果需要可分别消毒。所有培养基组分可培养开始时就存在,或者可以任选连续或分批加入。
每个实验分别规定培养条件。温度应为15℃-45℃。培养温度可保持恒定或实验中改变。培养基的pH应为5-8.5、优选约7.0,可在培养基中加入缓冲剂维持pH。用于该目的的典型缓冲剂为磷酸钾缓冲剂。可选择使用或同时使用合成缓冲剂如MOPS、HEPES、ACES等。也可以在培养期间加入氢氧化钠或氢氧化铵维持恒定培养pH。如果使用复合培养基组分如酵母提取物,对额外缓冲剂的需要可能降低,因为实际上许多复合化合物具有高缓冲能力。如果使用发酵罐培养所述微生物,也可以使用氨气控制pH。
培养时间通常为数小时至数天。选择培养时间以使液体培养基累积的产物量最多。可用各种容器进行所公开的使用,例如微量滴定板、玻璃管、玻璃瓶或不同大小的玻璃或金属发酵罐。为了筛选大量克隆,应用微量滴定板、玻璃管或摇瓶(带有或没有挡板)。优选使用100ml摇瓶,其中装以10%(体积)需要的生长培养基。培养瓶应在摇床(振幅25mm)上以100-300rpm速度范围振摇。维持湿润气氛使蒸发丧失最少;或者应进行精确校正蒸发损失。
如果测试遗传改进克隆,也应该测试未改良的对照克隆或含没有任何插入片段的基础质粒的对照克隆。利用已经于30℃温育的琼脂平板如CM平板(10g/l葡萄糖、2.5g/l氯化钠、2g/l尿素、10g/l聚胨、5g/l酵母提取物、5g/l肉膏、22g/l氯化钠、2g/l尿素、10g/l聚胨、5g/l酵母提取物、5g/l肉膏、22g/l琼脂,用2M氢氧化钠调至pH6.8)上的细胞接种培养基至OD600为0.5-1.5。加入得自CM平板的谷氨酸棒杆菌细胞的盐水悬浮液或加入该菌的液体预培养物,从而完成培养基接种。实施例8:体外分析突变蛋白的功能
本领域已有明确的酶活性和动力学参数测定法。必须使测定任何给定改变酶活性的实验适合野生型酶的比活,这完全在本领域一般技术人员能力范围内。关于酶的一般介绍以及涉及结构、动力学、原理、方法、应用的具体细节和许多酶活性测定实例可见于例如以下文献:Dixon,M.和Webb,E.C.,(1979)Enzymes,Longmans:London;Fersht,(1985)Enzyme Structure and Mechanism,Freeman:纽约;Walsh,(1979)Enzymatic Reaction Mechanisms.Freeman:San Francisco;Price,N.C.,Stevens,L.(1982)Fundamentals of Enzymology.Oxford Univ.Press:Oxford;Boyer,P.D.编著(1983)The Enzymes,第三版,Academic Press:纽约;Bisswanger,H.,(1994)Enzymkinetik,第二版,VCH:Weinheim(ISBN 3527300325);Bergmeyer,H.U.,Bergmeyer,J.,Graβ1,M.编著(1983-1986)Methods of Enzymatic Analysis,第三版,第I-XII卷,VerlagChemie:Weinheim;以及Ullmann’s Encyclopedia of Industrial Chemistry(1987),第A9卷,“Enzymes”,VCH:Weinheim,第352-363页。
可应用数种明确的方法如DNA条带移位测定(也称为凝胶阻滞测定)测定结合DNA的蛋白活性。可用报道基因测定(例如Kolmar,H.等(1995)EMBO J.14:3895-3904以及其中的引用文献介绍的测定)测量所述蛋白对其它分子表达的影响。报道基因测试系统是众所周知的而且明确用于原核生物细胞和真核生物细胞,利用酶如β半乳糖苷酶、绿色荧光蛋白等。
按照例如Gennis,R.B.(1989)“孔穴、通道与转运蛋白”,载于:Biomembranes,Molecular Structure and Function,Springer:Heidelberg,第85-137、199-234和270-322页介绍的方法,可测定膜转运蛋白的活性。实施例9:分析突变蛋白对需要产物产生的影响
可如下检测谷氨酸棒杆菌的遗传改进对需要化合物(例如氨基酸)产生的影响:使改良微生物在合适条件(例如上述条件)下生长,然后分析所述培养基和/或细胞组分中的需要产物(即氨基酸)的产量增加情况。所述分析技术是本领域一般技术人员周知的,包括光谱检测法、薄层层析、各种染色法、酶以及微生物学方法、以及分析型层析法如高效液相层析(参见例如Ullman,Encyclopedia of Industrial Chemistry,第A2卷,第89-90和443-613页,VCH:Weinheim(1985);Fallon,A.等,(1987)“HPLC在生物化学中的应用”,载于:Laboratory Techiquesin Biochemistry and Molecular Biology,第17卷;Rehm等(1993)Biotechnology,第3卷,第III章:“产物的回收与纯化”,第469-714页,VCH:Weinheim;Belter,PA.等(1988)Bioseparations:downstreamprocessing for biotechnology,John Wiley和Sons;Kennedy,J.F.和Cabral,J.M.S.(1992)Recovery processes for biological materials,John Wiley和Sons;Shaeiwitz,J.A.和Henry,J.D.(1988)生物化学分离,载于:Ulmann’s Encyclopedia of Industrial Chemistry,第B3卷,第11章,第1-27页,VCH:Weinheim;以及Dechow,F.J.(1989)Separation andpurification techniques in biotechnology,Noyes Publications)。
除了检测发酵终产物之外,也可以分析用以产生需要产物的代谢途径的其它组分,例如中间体和副产物,以确定产生所述化合物的总体效率。分析方法包括测量培养基中的营养素水平(例如糖、烃类、氮源、磷酸盐和其它离子)、检测生物量组成和生长、分析生物合成途径共同代谢物的产量以及测量发酵期间产生的气体。这些检测的标准方法概述于Applied Microbial Physiology,A Practical Approach,P.M.Rhodes和P.F.Stanbury编著,IRL Press,第103-129、131-163和165-192页(ISBN:0199635773)以及其中引用的参考文献。实施例10:从谷氨酸棒杆菌培养物纯化需要产物
可用本领域周知的各种方法从上述培养物的谷氨酸棒杆菌细胞或上清液中回收需要产物。如果需要产物不从细胞中分泌出来,可以通过低速离心从培养物收获细胞,应用标准方法裂解细胞,例如机械力或超声。离心去除细胞碎片,保留含有所述可溶性蛋白的上清液部分供进一步纯化所需要的化合物。如果产物从谷氨酸棒杆菌细胞分泌出来,则通过低速离心从所述培养物中去除细胞,保留上清液部分供进一步纯化。
使得自任一纯化方法的上清液部分在合适树脂上进行层析,或者需要分子保留在层析树脂上而样品中的大多数杂质被洗出,或者杂质保留在树脂上而需要分子样品被洗出。根据需要可重复所述层析步骤,使用相同或不同层析树脂。本领域一般技术人员在选择合适层析树脂和将所述树脂最有效地应用于待纯化的特定分子方面是非常精通的。纯化产物可通过过滤或超滤浓缩,贮藏在产物最稳定的温度状态下。
有许多本领域已知的纯化方法,上述纯化方法并不是限制性的。这样的纯化方法描述于例如Bailey,J.E.& Ollis,D.F.,BiochemicalEngineering Fundamentals,McGraw-Hill:纽约(1986)。
应用本领域的标准技术可检测分离化合物的身份和纯度。所述标准技术包括高效液相色谱(HPLC)、光谱法、染色法、薄层层析、NIRS、酶测定法或微生物测定法。所述分析方法的综述见:Patek等(1994)Appl.Environ.Microbiol.60:133-140;Malakhova等(1996)Biotekhnologiya 11:27-32;以及Schmidt等(1998)Bioprocess Engineer,19:67-70;Ulmann’sEncyclopedia of Industrial Chemistry,(1996)第A27卷,VCH:Weinheim,第89-90、521-540、540-547、559-566、575-581和581-587页;Michal,G.(1999)Biochemical Pathways:An Atlas of biochemistry and MolecularBiology,John Wiley and Sons;Fallon,A.等(1987)HPLC在生物化学中的应用,载于:Laboratory Techniques in Biochemistry and Molecular Biology,第17卷。实施例11:分析本发明的基因序列
比较序列并确定两种序列的同源性百分率是本领域已知的技术,可以采用数学算法完成,例如Karlin和Altschul的算法((1990)Proc.Natl.Acad.Sci.USA 87:2264-68)及其改良算法(Karlin和Altschul(1993)Proc.Natl.Acad.Sci.USA 90:5873-77。这种算法加入了Altschul等(1990)J.Mol.Biol.215:403-10的NBLAST和XBLAST程序(2.0版)。可应用NBLAST程序,打分=100,字串长度=12进行BLAST核苷酸检索,以便获得与本发明SMP核酸分子同源的核苷酸序列。可应用XBLAT程序,打分=50,字串长度=3进行BLAST蛋白质检索,以便获得与本发明SMP蛋白分子同源的氨基酸序列。为了获得比较目的的带空位序列对比,可按照Altschul等,(1997)Nucleic Acids Res.25(17):3389-3402所述,使用Gapped BLAST。当使用BLAST和Gapped BLAST程序时,本领域技术人员将知道如何优化用于分析特定序列的程序(例如XBLAST和NBLAST)参数。
用于序列比较的另一个数学算法实例是Meyers和Miller算法((1988)Comput.Appl.Biosci.4:11-17)。这种算法加入了ALIGN程序(2.0版),ALIGN程序是GCG序列对比软件包的组成部分。当使用ALIGN程序比较氨基酸序列时,可使用PAM120权重残基表、空位长度罚分12和空位罚分4。其它序列分析算法是本领域已知的,包括Torelli和Robotti(1994)Comput.Appl.Biosci.10:3-5介绍的ADVANCE和ADAM;以及Pearson和Lipman(1988)P.N.A.S.85:2444-8介绍的FASTA。
也可以使用GCG软件包中的GAP程序(可在http://www.gcg.com获得),选用Blosum 62矩阵或PAM250矩阵以及空位权重为12、10、8、6或4而长度权重为2、3或4,获得两种氨基酸序列的同源性百分率计算。应用GCG软件包中的GAP程序,使用标准参数,例如空位权重50而长度权重3,可获得两种核酸序列的同源性百分率。采用本领域已知的方法(参见例如Bexevanis和Ouellette编著(1998)Bioinformatics:A Practical Guide to the Analysis of Genes and Proteins,John Wiley和Sons:纽约),可进行本发明基因序列与Genbank序列的比较分析。以3步骤方法比较本发明基因序列与Genbank序列。第一步,对本发明各序列与Genbank核苷酸序列进行BLASTN分析(例如局部对比分析),保留前500个命中序列供进一步分析。第二步,对这500个命中序列进行FASTA检索(例如联合局部和全局序列的综合对比分析,其中排列对比序列限定区域)。最后,采用GCG软件包中的GAP程序(使用标准参数),对本发明各基因序列与前3个FASTA命中序列中的每一个进行全局性序列对比。为了获得正确的结果,应用本领域周知的方法使从Genbank取出的序列长度调整为查询序列长度。分析结果见表4。获得的数据与单独对本发明各基因与Genbank各参考序列进行GAP(全局)分析获得的数据相同,但是与这样的数据库-宽GAP(全局)分析相比,需要的计算时间显著减少。没有获得高于阈值的序列对比的本发明序列在表4中标示为没有序列对比信息。进而本领域技术人员应该知道的是,表4表头“同源性(%)(GAP)”下的GAP序列对比同源性百分率是以欧洲数字格式列出的,其中“,”代表小数点。例如该栏中的数值“40,345”是指“40.345%”。实施例12:构建和操作DNA微阵列
此外,本发明序列可以用于构建和操作DNA微阵列(DNA阵列的设计、方法学以及使用是本领域众所周知的,例如在以下文献中有其介绍:Schena,M.等(1995)Science 270:467-470;Wodicka,L.等(1997)Nature Biotechnology 15:1359-1367;DeSaizieu,A.等(1998)NatureBiotechnology 16:45-48;以及DeRisi,J.L.等(1997)Science 278:680-686)。
DNA微阵列是固体或柔性支持物,由硝酸纤维素、尼龙、玻璃、硅酮或其它材料组成。核酸分子与支持物表面有序结合。适当标记后,其它核酸或核酸混合物可与固定核酸分子杂交,标记物可用来监测和测量确定区域中的杂交分子的各信号强度。该方法可同时定量所加核酸样品或混合物中全部或选定核酸的相对量或绝对量。因此,DNA微阵列可平行分析多个(多达6800个或更多)核酸的表达(参见例如Schena,M.(1996)BioEssays 18(5):427-431)。
本发明序列可用来设计寡核苷酸引物,该寡核苷酸引物能够通过诸如聚合酶链式反应的核酸扩增反应扩增一种或多种谷氨酸棒杆菌基因的规定区域。选择和设计5’或3’寡核苷酸引物或合适接头可使产生的PCR产物与上述支持物介质表面共价连接(此外,参见例如Schena,M.等(1995)Science 270:467-470介绍)。
按照Wodicka,L.等(1997)Nature Biotechnology 15:1359-1367介绍,也可以通过原位寡核苷酸合成构建核酸微阵列。应用照相制板法使基体精确定义区曝光。由此激活光不稳定保护基团,并加入核苷酸,而避光区则不会发生任何改变。随后的保护和光激活循环使得在定义区合成不同寡核苷酸。通过固相寡核苷酸合成可在微阵列上合成本发明基因限定区。
样品或核苷酸混合物中存在的本发明核酸分子可与所述微阵列杂交。按照标准方法可标记这些核酸分子。简而言之,例如逆转录或DNA合成时通过掺入同位素或荧光标记核苷酸,从而标记核酸分子(例如mRNA分子或DNA分子)。已有关于标记核酸与微阵列杂交的介绍(例如载于:Schena,M等(1995)同上;Wokicka,L等(1997),同上;以及DeSaizieu A.等(1998),同上)。杂交分子的检测和定量须适合特定掺入标记物。例如按照Schena,M等(1995)(同上)介绍可检测放射性标记物,而例如按照Shalon等((1996)Genome Research 6:639-645)的方法可检测荧光标记物。
如上所述,将本发明序列用于DNA微阵列技术可比较分析谷氨酸棒杆菌或其它棒杆菌属的不同菌株。例如,核酸阵列方法学有助于根据各个转录物分布研究菌株内变异和鉴定对于特定和/或所需菌株特性如致病性、生产能力和胁迫耐受性重要的基因。此外,利用核酸阵列方法可以比较发酵期间本发明基因的表达分布。实施例13:分析细胞蛋白群动力学(蛋白质组学(Proteomics))
本发明基因、组合物和方法可用于研究蛋白群相互作用和动力学,称为“蛋白质组学”。目的蛋白群包括但不限于谷氨酸棒杆菌总蛋白群(例如与其它生物体的蛋白群比较)、在特定环境或代谢条件下(例如发酵期间的高温或低温,或者高pH或低pH)具有活性的蛋白、在培养和发展特定期具有活性的蛋白。
可用本领域周知的方法如凝胶电泳分析蛋白质群。例如通过裂解或提取可获得细胞蛋白,应用各种电泳技术可使蛋白群彼此分开。十二烷基硫酸钠凝胶电泳(SDS-PAGE)主要根据蛋白分子量分离蛋白。等电聚焦聚丙烯酰胺凝胶电泳(IEF-PAGE)通过蛋白等电点(它不仅反映氨基酸序列,而且反映所述蛋白翻译后修饰)分离蛋白。另一个更优选的蛋白分析方法是IEF-PAGE和SDS-PAGE的顺序组合,称为2-D-gel电泳(例如见以下文献介绍:Hermann等(1998)Electrophoresis 19:3217-3221;Fountoulakis等(1998)Electrophoresis 19:1193-1202;Langen等(1997)Electrophoresis 18:1184-1192;Antelmann等(1997)Electrophoresis 18:1451-1463)。也可以使用其它蛋白分析方法进行蛋白质分离,例如毛细管电泳;所述技术是本领域周知的技术。
可用标准技术如染色或标记,显现所述方法分开的蛋白。合适染料是本领域已知的,包括考马斯亮蓝、银染料或荧光染料如SyproRuby(Molecular Probes)。在谷氨酸棒杆菌培养基中含有放射性标记氨基酸或其它蛋白前体(例如35S-甲硫氨酸、35S-半胱氨酸、14C-标记氨基酸、15N-标记氨基酸、
或
或13C-标记氨基酸)可在其分离前标记这些细胞的蛋白。同样可使用荧光标记物。可按照前述方法提取、分开和分离所述标记蛋白。
通过测量所用染料或标记物量,可进一步分析所述技术显现的蛋白。采用例如光学方法可定量测定给定蛋白量,而且可以与相同凝胶或其它凝胶上的其它蛋白量比较。例如采用光学比较、光谱法、图象扫描和凝胶分析、或者通过应用照相胶片和屏幕,可在凝胶上进行蛋白比较。这样的技术是本领域周知的。
为了确定任何给定蛋白的身份,可采用直接测序或其它标准技术。可使用例如N-和/或C-末端氨基酸测序(例如Edman降解),也可以使用质谱法(尤其是MALDI或ESI技术(参见例如Langen等(1997)Electrophoresis 18:1184-1192))。本发明提供的蛋白序列可用于通过所述技术鉴定谷氨酸棒杆菌蛋白。
所述方法获得的信息可用于比较各种生物条件(例如不同生物、不同发酵时间点、不同培养基条件或不同群落生境等)的不同样品之间的蛋白存在模式、蛋白活性或蛋白修饰。这些实验单独或与其它技术联合获得的资料可用于各种用途,例如比较给定(例如代谢)情况下各种生物的行为、提高生产精细化学品的菌株的生产能力或提高生产精细化学品的产率。等同实施方案
本领域技术人员知道或者仅采用常规实验就能够确定本发明介绍的具体实施方案的许多等同实施方案。以下的权利要求书包括这样的等同实施方案。
CPCH0163723P 说明书的氨基酸和核苷酸序列表
序列表<110>BASF Aktiengesellschaft<120>编码参与碳代谢和能量产生的蛋白质的谷氨酸棒杆菌基因<130>BGI-126CPPC<140><141><160>782<210>1<211>828<212>DNA<213>谷氨酸棒杆菌(Corynebacterium glutamicum)<220><221>CDS<222>(101)..(805)<223>RXS02735<400>1gaggagcttc gccacatgga tccagatttg ggctaccagc acgcactatc cggcttgtcc 60agcgtcaagc tggaaaccgt ctaaggagaa atacaacact atg gtt gat gta gta 115
Met Val Asp Val Val
1 5cgc gca cgc gat act gaa gat ttg gtt gca cag gct gcc tcc aaa ttc 163Arg Ala Arg Asp Thr Glu Asp Leu Val Ala Gln Ala Ala Ser Lys Phe
10 15 20att gag gtt gtt gaa gca gca act gcc aat aat ggc acc gca cag gta 211Ile Glu Val Val Glu Ala Ala Thr Ala Asn Asn Gly Thr Ala Gln Val
25 30 35gtg ctc acc ggt ggt ggc gcc ggc atc aag ttg ctg gaa aag ctc agc 259Val Leu Thr Gly Gly Gly Ala Gly Ile Lys Leu Leu Glu Lys Leu Ser
40 45 50gtt gat gcg gct gac ctt gcc tgg gat cgc att cat gtg ttc ttc ggc 307Val Asp Ala Ala Asp Leu Ala Trp Asp Arg Ile His Val Phe Phe Gly
55 60 65gat gag cgc aat gtc cct gtc agt gat tct gag tcc aat gag ggc cag 355Asp Glu Arg Asn Val Pro Val Ser Asp Ser Glu Ser Asn Glu Gly Gln70 75 80 85gct cgt gag gca ctg ttg tcc aag gtt tct atc cct gaa gcc aac att 403Ala Arg Glu Ala Leu Leu Ser Lys Val Ser Ile Pro Glu Ala Asn Ile
90 95 100cac gga tat ggt ctc ggc gac gta gat ctt gca gag gca gcc cgc gct 451His Gly Tyr Gly Leu Gly Asp Val Asp Leu Ala Glu Ala Ala Arg Ala
105 110 115tac gaa gct gtg ttg gat gaa ttc gca cca aac ggc ttt gat ctt cac 499Tyr Glu Ala Val Leu Asp Glu Phe Ala Pro Asn Gly Phe Asp Leu His
120 125 130ctg ctc ggc atg ggt ggc gaa ggc cat atc aac tcc ctg ttc cct cac 547Leu Leu Gly Met Gly Gly Glu Gly His Ile Asn Ser Leu Phe Pro His
135 140 145acc gat gca gtc aag gaa tcc tcc gca aag gtc atc gcg gtg ttt gat 595Thr Asp Ala Val Lys Glu Ser Ser Ala Lys Val Ile Ala Val Phe Asp150 155 160 165tcc cct aag cct cct tca gag cgt gca act cta acc ctt cct gcg gtt 643Ser Pro Lys Pro Pro Ser Glu Arg Ala Thr Leu Thr Leu Pro Ala Val
170 175 180cac tcc gca aag cgc gtg tgg ttg ctg gtt tct ggt gcg gag aag gct 691His Ser Ala Lys Arg Val Trp Leu Leu Val Ser Gly Ala Glu Lys Ala
185 190 195gag gca gct gcg gcg atc gtc aac ggt gag cct gct gtt gag tgg cct 739Glu Ala Ala Ala Ala Ile Val Asn Gly Glu Pro Ala Val Glu Trp Pro
200 205 210gct gct gga gct acc gga tct gag gaa acg gta ttg ttc ttg gct gat 787Ala Ala Gly Ala Thr Gly Ser Glu Glu Thr Val Leu Phe Leu Ala Asp
215 220 225gat gct gca gga aat ctc taagcagcgc cagctctaac aag 828Asp Ala Ala Gly Asn Leu230 235<210>2<211>235<212>PRT<213>谷氨酸棒杆菌<400>2Met Val Asp Val Val Arg Ala Arg Asp Thr Glu Asp Leu Val Ala Gln1 5 10 15Ala Ala Ser Lys Phe Ile Glu Val Val Glu Ala Ala Thr Ala Asn Asn
20 25 30Gly Thr Ala Gln Val Val Leu Thr Gly Gly Gly Ala Gly Ile Lys Leu
35 40 45Leu Glu Lys Leu Ser Val Asp Ala Ala Asp Leu Ala Trp Asp Arg Ile
50 55 60His Val Phe Phe Gly Asp Glu Arg Asn Val Pro Val Ser Asp Ser Glu65 70 75 80Ser Asn Glu Gly Gln Ala Arg Glu Ala Leu Leu Ser Lys Val Ser Ile
85 90 95Pro Glu Ala Asn Ile His Gly Tyr Gly Leu Gly Asp Val Asp Leu Ala
100 105 110Glu Ala Ala Arg Ala Tyr Glu Ala Val Leu Asp Glu Phe Ala Pro Asn
115 120 125Gly Phe Asp Leu His Leu Leu Gly Met Gly Gly Glu Gly His Ile Asn
130 135 140Ser Leu Phe Pro His Thr Asp Ala Val Lys Glu Ser Ser Ala Lys Val145 150 155 160Ile Ala Val Phe Asp Ser Pro Lys Pro Pro Ser Glu Arg Ala Thr Leu
165 170 175Thr Leu Pro Ala Val His Ser Ala Lys Arg Val Trp Leu Leu Val Ser
180 185 190Gly Ala Glu Lys Ala Glu Ala Ala Ala Ala Ile Val Asn Gly Glu Pro
195 200 205Ala Val Glu Trp Pro Ala Ala Gly Ala Thr Gly Ser Glu Glu Thr Val
210 215 220Leu Phe Leu Ala Asp Asp Ala Ala Gly Asn Leu225 230 235<210>3<211>468<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(445)<223>RXA01626<400> 3gcaatagcga atgcgtaatt aaccacactt caaacctagc ccctcaggtg gaggattccg 60acattaccgt ctgaaaaatt tcatccgtag gctaaagagc atg tcg aaa acg atc 115
Met Ser Lys Thr Ile
1 5atc gtg cgc acc gaa att gaa atc cct gga cac cca acc gcc atc cat 163Ile Val Arg Thr Glu Ile Glu Ile Pro Gly His Pro Thr Ala Ile His
10 15 20atc gca gag atg cag gag ctt ccc cca tct gag gct caa ggc ggc gtg 211Ile Ala Glu Met Gln Glu Leu Pro Pro Ser Glu Ala Gln Gly Gly Val
25 30 35cag atg tgc aaa atg cag cgc att att gaa cta gca gga act gcc gaa 259Gln Met Cys Lys Met Gln Arg Ile Ile Glu Leu Ala Gly Thr Ala Glu
40 45 50ggg gat gtc gtt act ggt gca ggt gtt att ggc gga tct aat ttc cag 307Gly Asp Val Val Thr Gly Ala Gly Val Ile Gly Gly Ser Asn Phe Gln
55 60 65ctg aat aac gag cca aat gaa gtg gtt ccc cat cca gat acc tat gcg 355Leu Asn Asn Glu Pro Asn Glu Val Val Pro His Pro Asp Thr Tyr Ala70 75 80 85gat ttc ccc gat atc aag gcg gtt gtt atc tcc gcg gag act ttt gaa 403Asp Phe Pro Asp Ile Lys Ala Val Val Ile Ser Ala Glu Thr Phe Glu
90 95 100ggc ctg tgg ctg gaa gcg gga gcg aag ttc cct ggg tta aat 445Gly Leu Trp Leu Glu Ala Gly Ala Lys Phe Pro Gly Leu Asn
105 110 115taaccacttg cagtataccc tag 468<210>4<211>115<212>PRT<213>谷氨酸棒杆菌<400>4Met Ser Lys Thr Ile Ile Val Arg Thr Glu Ile Glu Ile Pro Gly His1 5 10 15Pro Thr Ala Ile His Ile Ala Glu Met Gln Glu Leu Pro Pro Ser Glu
20 25 30Ala Gln Gly Gly Val Gln Met Cys Lys Met Gln Arg Ile Ile Glu Leu
35 40 45Ala Gly Thr Ala Glu Gly Asp Val Val Thr Gly Ala Gly Val Ile Gly
50 55 60Gly Ser Asn Phe Gln Leu Asn Asn Glu Pro Asn Glu Val Val Pro His65 70 75 80Pro Asp Thr Tyr Ala Asp Phe Pro Asp Ile Lys Ala Val Val Ile Ser
85 90 95Ala Glu Thr Phe Glu Gly Leu Trp Leu Glu Ala Gly Ala Lys Phe Pro
100 105 110Gly Leu Asn
115<210>5<211>780<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(757)<223>RXA02245<400>5gccacaccgc cacggcaccg atgcgatgtt tgtggcagtg ctgcgaaaga agtagacctg 60tgagctaagt ggggtagaca agagggctat gatttagggc atg gca caa cgt act 115
Met Ala Gln Arg Thr
1 5cca cta atc gcc cca tcc att ctt gct gct gat ttc tcc cgc tta ggg 163Pro Leu Ile Ala Pro Ser Ile Leu Ala Ala Asp Phe Ser Arg Leu Gly
10 15 20gag cag gtg ttg gct gtt cct gat gct gac tgg att cac gtc gac atc 211Glu Gln Val Leu Ala Val Pro Asp Ala Asp Trp Ile His Val Asp lle
25 30 35atg gac gga cae ttc gtt cca aac ttg agc ttt ggc gcg gat atc aca 259Met Asp Gly His Phe Val Pro Asn Leu Ser Phe Gly Ala Asp Ile Thr
40 45 50gct gcg gtc aac cgc gtt acg gac aaa gaa cta gac gtc cac ctg atg 307Ala Ala Val Asn Arg Val Thr Asp Lys Glu Leu Asp Val His Leu Met
55 60 65atc gaa aac cca gag aag tgg gtg gac aac tac atc gac gct ggc gcg 355Ile Glu Asn Pro Glu Lys Trp Val Asp Asn Tyr Ile Asp Ala Gly Ala70 75 80 85gac tgc att gtt ttc cac gtt gaa gcc acc gaa ggt cac gtt gag ttg 403Asp Cys Ile Val Phe His Val Glu Ala Thr Glu Gly His Val Glu Leu
90 95 100gct aag tac atc cgt tcc aag ggt gtg cgt gca ggt ttc tcc ctg cgc 451Ala Lys Tyr Ile Arg Ser Lys Gly Val Arg Ala Gly Phe Ser Leu Arg
105 110 115cct gga act ccc atc gag gat tac ttg gat gac ctc gag cac ttc gat 499Pro Gly Thr Pro Ile Glu Asp Tyr Leu Asp Asp Leu Glu His Phe Asp
120 125 130gaa gtc atc gtc atg agc gtc gag cct gga ttc ggt ggc caa agc ttc 547Glu Val Ile Val Met Ser Val Glu Pro Gly Phe Gly Gly Gln Ser Phe
135 140 145atg cct gaa caa ctg gaa aag gtt cgt acc ctg cgc aag gtc atc gat 595Met Pro Glu Gln Leu Glu Lys Val Arg Thr Leu Arg Lys Val Ile Asp150 155 160 165gag cgc ggt ctg aac acc gtc atc gag atc gac ggc ggc att agc gcc 643Glu Arg Gly Leu Asn Thr Val Ile Glu Ile Asp Gly Gly Ile Ser Ala
170 175 180aag acc atc aag cag gct gcc gac gct ggc gtg gat gcc ttc gtt gca 691Lys Thr Ile Lys Gln Ala Ala Asp Ala Gly Val Asp Ala Phe Val Ala
185 190 195ggt tcc gct gtg tac ggc gct gag gat ccc aac aag gcg atc cag gag 739Gly Ser Ala Val Tyr Gly Ala Glu Asp Pro Asn Lys Ala Ile Gln Glu
200 205 210ttg cga gca ctc gcg cag taaatggatg ttgcgcacgc gtt 780Leu Arg Ala Leu Ala Gln
215<210>6<211>219<212>PRT<213>谷氨酸棒杆菌<400>6Met Ala Gln Arg Thr Pro Leu Ile Ala Pro Ser Ile Leu Ala Ala Asp1 5 10 15Phe Ser Arg Leu Gly Glu Gln Val Leu Ala Val Pro Asp Ala Asp Trp
20 25 30Ile His Val Asp Ile Met Asp Gly His Phe Val Pro Asn Leu Ser Phe
35 40 45Gly Ala Asp Ile Thr Ala Ala Val Asn Arg Val Thr Asp Lys Glu Leu
50 55 60Asp Val His Leu Met Ile Glu Asn Pro Glu Lys Trp Val Asp Asn Tyr65 70 75 80Ile Asp Ala Gly Ala Asp Cys Ile Val Phe His Val Glu Ala Thr Glu
85 90 95Gly His Val Glu Leu Ala Lys Tyr Ile Arg Ser Lys Gly Val Arg Ala
100 105 110Gly Phe Ser Leu Arg Pro Gly Thr Pro Ile Glu Asp Tyr Leu Asp Asp
115 120 125Leu Glu His Phe Asp Glu Val Ile Val Met Ser Val Glu Pro Gly Phe
130 135 140Gly Gly Gln Ser Phe Met Pro Glu Gln Leu Glu Lys Val Arg Thr Leu145 150 155 160Arg Lys Val Ile Asp Glu Arg Gly Leu Asn Thr Val Ile Glu Ile Asp
165 170 175Gly Gly Ile Ser Ala Lys Thr Ile Lys Gln Ala Ala Asp Ala Gly Val
180 185 190Asp Ala Phe Val Ala Gly Ser Ala Val Tyr Gly Ala Glu Asp Pro Asn
195 200 205Lys Ala Ile Gln Glu Leu Arg Ala Leu Ala Gln
210 215<210>7<211>442<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(442)<223>RXA01015<400>7ccaagattga gtcgagctat tttgcgattt gtgaaccccc aaatagggga aaagtccggg 60tatccgccgt tgtgaaaatg cctgcagtaa actgacttcc atg cgc gta tac ctt 115
Met Arg Val Tyr Leu
1 5gga gca gac cac gct ggt ttc gaa act aaa aat gca atc gca gaa cac 163Gly Ala Asp His Ala Gly Phe Glu Thr Lys Asn Ala Ile Ala Glu His
10 15 20ctt aag gcc cac ggc cac gaa gtg atc gac tgc gga gcc cac acc tat 211Leu Lys Ala His Gly His Glu Val Ile Asp Cys Gly Ala His Thr Tyr
25 30 35gat gca gaa gat gac tac cca gcc ttc tgc atc gaa gca gct agc cgc 259Asp Ala Glu Asp Asp Tyr Pro Ala Phe Cys Ile Glu Ala Ala Ser Arg
40 45 50aca gta aac gac cca ggc tca ctc ggc atc gtc ctg ggt gga tcc gga 307Thr Val Asn Asp Pro Gly Ser Leu Gly Ile Val Leu Gly Gly Ser Gly
55 60 65aac ggc gag cag atc gcc gcc aac aag gtc aag ggt gca cgt tgt gca 355Asn Gly Glu Gln Ile Ala Ala Asn Lys Val Lys Gly Ala Arg Cys Ala70 75 80 85ctt gct tgg tct gtt gaa act gca cgc ctc gcc cgc gag cac aac aat 403Leu Ala Trp Ser Val Glu Thr Ala Arg Leu Ala Arg Glu His Asn Asn
90 95 100gcg aac ctc atc ggc atc ggc ggc cgc atg cac tca gag 442Ala Asn Leu Ile Gly Ile Gly Gly Arg Met His Ser Glu
105 110<210>8<211>114<212>PRT<213>谷氨酸棒杆菌<400>8Met Arg Val Tyr Leu Gly Ala Asp His Ala Gly Phe Glu Thr Lys Asn1 5 10 15Ala Ile Ala Glu His Leu Lys Ala His Gly His Glu Val Ile Asp Cys
20 25 30Gly Ala His Thr Tyr Asp Ala Glu Asp Asp Tyr Pro Ala Phe Cys Ile
35 40 45Glu Ala Ala Ser Arg Thr Val Asn Asp Pro Gly Ser Leu Gly Ile Val
50 55 60Leu Gly Gly Ser Gly Asn Gly Glu Gln Ile Ala Ala Asn Lys Val Lys65 70 75 80Gly Ala Arg Cys Ala Leu Ala Trp Ser Val Glu Thr Ala Arg Leu Ala
85 90 95Arg Glu His Asn Asn Ala Asn Leu Ile Gly Ile Gly Gly Arg Met His
100 105 110Ser Glu<210>9<211>2142<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2119)<223>RXN01312<400>9gcagttgcgt acatcgttcc tgcactggtc ctgatcggca acatcaccat tccgttcgcc 60atcgctgttg gttggattgc gtaaaggtta ggaagaattt atg agc act cac tct 115
Met Ser Thr His Ser
1 5gaa acc acc cgc cca gag ttc arc cac cca gtc tca gtc ctc cca gag 163Glu Thr Thr Arg Pro Glu Phe Ile His Pro Val Ser Val Leu Pro Glu
10 15 20gtc tca gct ggt acg gtc ctt gac gct gca gag cca gca ggc gtt ccc 211Val Ser Ala Gly Thr Val Leu Asp Ala Ala Glu Pro Ala Gly Val Pro
25 30 35acc aaa gat atg tgg gaa tac caa aaa gac cac atg aac ctg gtc tcc 259Thr Lys Asp Met Trp Glu Tyr Gln Lys Asp His Met Asn Leu Val Ser
40 45 50cca ctg aac cga cgc aag ttc cgt gtc ctc gtc gtt ggc acc ggc ctg 307Pro Leu Asn Arg Arg Lys Phe Arg Val Leu Val Val Gly Thr Gly Leu
55 60 65tcc ggt ggt gct gca gca gca gcc ctc ggc gaa ctc gga tac gac gtc 355Ser Gly Gly Ala Ala Ala Ala Ala Leu Gly Glu Leu Gly Tyr Asp Val70 75 80 85aag gcg ttc acc tac cac gac gca cct cgc cgt gcg cac tcc att gct 403Lys Ala Phe Thr Tyr His Asp Ala Pro Arg Arg Ala His Ser Ile Ala
90 95 100gca cag ggt ggc gtt aac tcc gcc cgc ggc aag aag gta gac aac gac 451Ala Gln Gly Gly Val Asn Ser Ala Arg Gly Lys Lys Val Asp Asn Asp
105 110 115ggc gca tac cgc cac gtc aag gac acc gtc aag ggc ggc gac tac cgt 499Gly Ala Tyr Arg His Val Lys Asp Thr Val Lys Gly Gly Asp Tyr Arg
120 125 130ggt cgc gag tcc gac tgc tgg cgt ctc gcc gtc gag tcc gtc cgc gtc 547Gly Arg Glu Ser Asp Cys Trp Arg Leu Ala Val Glu Ser Val Arg Val
135 140 145atc gac cac atg aac gcc atc ggt gca cca ttc gcc cgc gaa tac ggt 595Ile Asp His Met Asn Ala Ile Gly Ala Pro Phe Ala Arg Glu Tyr Gly150 155 160 165ggc gcc ttg gca acc cgt tcc ttc ggt ggt gtg cag gtc tcc cgt acc 643Gly Ala Leu Ala Thr Arg Ser Phe Gly Gly Val Gln Val Ser Arg Thr
170 175 180tac tac acc cgt gga caa acc gga cag cag ctg cag ttc tcc acc gca 691Tyr Tyr Thr Arg Gly Gln Thr Gly Gln Gln Leu Gln Phe Ser Thr Ala
185 190 195tcc gca cta cag cgc cag atc cac ctc ggc tcc gta gaa atc ttc acc 739Ser Ala Leu Gln Arg Gln Ile His Leu Gly Ser Val Glu Ile Phe Thr
200 205 210cat aac gaa atg gtt gac gtc att gtc acc gaa cgt aac ggt gaa aag 787His Asn Glu Met Val Asp Val Ile Val Thr Glu Arg Asn Gly Glu Lys
215 220 225cgc tgc gaa ggc ctg atc atg cgc aac ctg atc acc ggc gag ctc acc 835Arg Cys Glu Gly Leu Ile Met Arg Asn Leu Ile Thr Gly Glu Leu Thr230 235 240 245gca cac acc ggc cat gcc gtt atc ctg gca acc ggt ggc tac ggc aac 883Ala His Thr Gly His Ala Val Ile Leu Ala Thr Gly Gly Tyr Gly Asn
250 255 260gtg tac cac atg tcc acc ctg gcc aag aac tcc aac gcc tcg gcc atc 931Val Tyr His Met Ser Thr Leu Ala Lys Asn Ser Asn Ala Ser Ala Ile
265 270 275atg cgt gca tac gaa gcc ggc gca tac ttc gcg tcc cca tcg ttc atc 979Met Arg Ala Tyr Glu Ala Gly Ala Tyr Phe Ala Ser Pro Ser Phe Ile
280 285 290cag ttc cac cca acc ggc ctg cct gtg aac tcc acc tgg cag tcc aag 1027Gln Phe His Pro Thr Gly Leu Pro Val Asn Ser Thr Trp Gln Ser Lys
295 300 305acc att ctg atg tcc gag tcg ctg cgt aac gac ggc cgc atc tgg tcc 1075Thr Ile Leu Met Ser Glu Ser Leu Arg Asn Asp Gly Arg Ile Trp Ser310 315 320 325cct aag gaa ccg aac gat aac cgc gat cca aac acc atc cct gag gat 1123Pro Lys Glu Pro Asn Asp Asn Arg Asp Pro Asn Thr Ile Pro Glu Asp
330 335 340gag cgc gac tac ttc ctg gag cgc cgc tac cca gca ttc ggt aac ctc 1171Glu Arg Asp Tyr Phe Leu Glu Arg Arg Tyr Pro Ala Phe Gly Asn Leu
345 350 355gtc cca cgt gac gtt gct tcc cgt gcg atc tcc cag cag atc aat gct 1219Val Pro Arg Asp Val Ala Ser Arg Ala Ile Ser Gln Gln Ile Asn Ala
360 365 370ggt ctc ggt gtt gga cct ctg aac aac gct gca tac ctg gac ttc cgc 1267Gly Leu Gly Val Gly Pro Leu Asn Asn Ala Ala Tyr Leu Asp Phe Arg
375 380 385gac gcc acc gag cgc ctc gga cag gac acc atc cgc gag cgt tac tcc 1315Asp Ala Thr Glu Arg Leu Gly Gln Asp Thr Ile Arg Glu Arg Tyr Ser390 395 400 405aac ctc ttc acc atg tac gaa gag gca att ggc gag gac cca tac tcc 1363Asn Leu Phe Thr Met Tyr Glu Glu Ala Ile Gly Glu Asp Pro Tyr Ser
410 415 420agc cca atg cgt att gca ccg acc tgc cac ttc acc atg ggt ggc ctc 1411Ser Pro Met Arg Ile Ala Pro Thr Cys His Phe Thr Met Gly Gly Leu
425 430 435tgg act gac ttc aac gaa atg acg tca ctc cca ggt ctg ttc tgc gca 1459Trp Thr Asp Phe Ash Glu Met Thr Ser Leu Pro Gly Leu Phe Cys Ala
440 445 450ggc gaa gca tcc tgg acc tac cac ggt gca aac cgt ctg ggc gca aac 1507Gly Glu Ala Ser Trp Thr Tyr His Gly Ala Asn Arg Leu Gly Ala Asn
455 460 465tcc ctg ctc tcc gct tcc gtc gat ggc tgg ttc acc ctg cca ttc acc 1555Ser Leu Leu Ser Ala Ser Val Asp Gly Trp Phe Thr Leu Pro Phe Thr470 475 480 485atc cct aac tac ctc ggc cca ttg ctt ggc tcc gag cgt ctg tca gag 1603Ile Pro Asn Tyr Leu Gly Pro Leu Leu Gly Ser Glu Arg Leu Ser Glu
490 495 500gat gca cca gaa gca cag gca gcg att gcg cgt gca cag gct cgc att 1651Asp Ala Pro Glu Ala Gln Ala Ala Ile Ala Arg Ala Gln Ala Arg Ile
505 510 515gac cgc ctc atg ggc aac cgc cca gag tgg gtc ggt gac aac gtt cac 1699Asp Arg Leu Met Gly Asn Arg Pro Glu Trp Val Gly Asp Asn Val His
520 525 530gga cct gag tac tac cac cgc cag ctt ggc gat atc ctg tac ttc tcc 1747Gly Pro Glu Tyr Tyr His Arg Gln Leu Gly Asp Ile Leu Tyr Phe Ser
535 540 545tgt ggc gtt tcc cga aac gta gaa gac ctc cag gat ggc atc aac aag 1795Cys Gly Val Ser Arg Asn Val Glu Asp Leu Gln Asp Gly Ile Asn Lys550 555 560 565atc cgt gcc ctc cgc gat gac ttc tgg aag aac atg cgc atc acc ggc 1843Ile Arg Ala Leu Arg Asp Asp Phe Trp Lys Asn Met Arg Ile Thr Gly
570 575 580agc acc gat gag atg aac cag gtt ctc gaa tac gca gca cgc gta gcc 1891Ser Thr Asp Glu Met Asn Gln Val Leu Glu Tyr Ala Ala Arg Val Ala
585 590 595gac tac atc gac ctc ggc gaa ctc atg tgt gtc gac gcc ctc gac cgc 1939Asp Tyr Ile Asp Leu Gly Glu Leu Met Cys Val Asp Ala Leu Asp Arg
600 605 610gac gag tcc tgt ggc gct cac ttc cgc gac gac cac ctc tcc gaa gat 1987Asp Glu Ser Cys Gly Ala His Phe Arg Asp Asp His Leu Ser Glu Asp
615 620 625ggc gaa gca caa cgt gac gac caa aac tgg tgc ttc gtc tcc gca tgg 2035Gly Glu Ala Gln Arg Asp Asp Gln Asn Trp Cys Phe Val Ser Ala Trp630 635 640 645gaa cca ggc gag aat gga acc ttc gtc tgc cac gca gaa cca ctg ttc 2083Glu Pro Gly Glu Asn Gly Thr Phe Val Cys His Ala Glu Pro Leu Phe
650 655 660ttc gaa tct gtc cca ctg cag aca agg aac tac aag taatgaaact 2129Phe Glu Ser Val Pro Leu Gln Thr Arg Asn Tyr Lys
665 670tacacttgag atc 2142<210>10<211>673<212>PRT<213>谷氨酸棒杆菌<400>10Met Ser Thr His Ser Glu Thr Thr Arg Pro Glu Phe Ile His Pro Val1 5 10 15Ser Val Leu Pro Glu Val Ser Ala Gly Thr Val Leu Asp Ala Ala Glu
20 25 30Pro Ala Gly Val Pro Thr Lys Asp Met Trp Glu Tyr Gln Lys Asp His
35 40 45Met Asn Leu Val Ser Pro Leu Asn Arg Arg Lys Phe Arg Val Leu Val
50 55 60Val Gly Thr Gly Leu Ser Gly Gly Ala Ala Ala Ala Ala Leu Gly Glu65 70 75 80Leu Gly Tyr Asp Val Lys Ala Phe Thr Tyr His Asp Ala Pro Arg Arg
85 90 95Ala His Ser Ile Ala Ala Gln Gly Gly Val Asn Ser Ala Arg Gly Lys
100 105 110Lys Val Asp Asn Asp Gly Ala Tyr Arg His Val Lys Asp Thr Val Lys
115 120 125Gly Gly Asp Tyr Arg Gly Arg Glu Ser Asp Cys Trp Arg Leu Ala Val
130 135 140Glu Ser Val Arg Val Ile Asp His Met Asn Ala Ile Gly Ala Pro Phe145 150 155 160Ala Arg Glu Tyr Gly Gly Ala Leu Ala Thr Arg Ser Phe Gly Gly Val
165 170 175Gln Val Ser Arg Thr Tyr Tyr Thr Arg Gly Gln Thr Gly Gln Gln Leu
180 185 190Gln Phe Ser Thr Ala Ser Ala Leu Gln Arg Gln Ile His Leu Gly Ser
195 200 205Val Glu Ile Phe Thr His Asn Glu Met Val Asp Val Ile Val Thr Glu
210 215 220Arg Asn Gly Glu Lys Arg Cys Glu Gly Leu Ile Met Arg Asn Leu Ile225 230 235 240Thr Gly Glu Leu Thr Ala His Thr Gly His Ala Val Ile Leu Ala Thr
245 250 255Gly Gly Tyr Gly Asn ValTyr His Met Ser Thr Leu Ala Lys Asn Ser
260 265 270Asn Ala Ser Ala Ile Met Arg Ala Tyr Glu Ala Gly Ala Tyr Phe Ala
275 280 285Ser Pro Ser Phe Ile Gln Phe His Pro Thr Gly Leu Pro Val Asn Ser
290 295 300Thr Trp Gln Ser Lys Thr Ile Leu Met Ser Glu Ser Leu Arg Asn Asp305 310 315 320Gly Arg Ile Trp Ser Pro Lys Glu Pro Asn Asp Asn Arg Asp Pro Asn
325 330 335Thr Ile Pro Glu Asp Glu Arg Asp Tyr Phe Leu Glu Arg Arg Tyr Pro
340 345 350Ala Phe Gly Asn Leu Val Pro Arg Asp Val Ala Ser Arg Ala Ile Ser
355 360 365Gln Gln Ile Asn Ala Gly Leu Gly Val Gly Pro Leu Asn Asn Ala Ala
370 375 380Tyr Leu Asp Phe Arg Asp Ala Thr Glu Arg Leu Gly Gln Asp Thr Ile385 390 395 400Arg Glu Arg Tyr Ser Asn Leu Phe Thr Met Tyr Glu Glu Ala Ile Gly
405 410 415Glu Asp Pro Tyr Ser Ser Pro Met Arg Ile Ala Pro Thr Cys His Phe
420 425 430Thr Met Gly Gly Leu Trp Thr Asp Phe Asn Glu Met Thr Ser Leu Pro
435 440 445Gly Leu Phe Cys Ala Gly Glu Ala Ser Trp Thr Tyr His Gly Ala Asn
450 455 460Arg Leu Gly Ala Asn Ser Leu Leu Ser Ala Ser Val Asp Gly Trp Phe465 470 475 480Thr Leu Pro Phe Thr Ile Pro Asn Tyr Leu Gly Pro Leu Leu Gly Ser
485 490 495Glu Arg Leu Ser Glu Asp Ala Pro Glu Ala Gln Ala Ala Ile Ala Arg
500 505 510Ala Gln Ala Arg Ile Asp Arg Leu Met Gly Asn Arg Pro Glu Trp Val
515 520 525Gly Asp Asn Val His Gly Pro Glu Tyr Tyr His Arg Gln Leu Gly Asp
530 535 540Ile Leu Tyr Phe Ser Cys Gly Val Ser Arg Asn Val Glu Asp Leu Gln545 550 555 560Asp Gly Ile Asn Lys Ile Arg Ala Leu Arg Asp Asp Phe Trp Lys Asn
565 570 575Met Arg Ile Thr Gly Ser Thr Asp Glu Met Asn Gln Val Leu Glu Tyr
580 585 590Ala Ala Arg Val Ala Asp Tyr Ile Asp Leu Gly Glu Leu Met Cys Val
595 600 605Asp Ala Leu Asp Arg Asp Glu Ser Cys Gly Ala His Phe Arg Asp Asp
610 615 620His Leu Ser Glu Asp Gly Glu Ala Gln Arg Asp Asp Gln Asn Trp Cys625 630 635 640Phe Val Ser Ala Trp Glu Pro Gly Glu Asn Gly Thr Phe Val Cys His
645 650 655Ala Glu Pro Leu Phe Phe Glu Ser Val Pro Leu Gln Thr Arg Asn Tyr
660 665 670Lys<210>11<211>1100<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1077)<223>FRXA01312<400>11gag tcg ctg cgt aac gac ggc cgc atc tgg tcc cct aag gaa ccg aac 48Glu Ser Leu Arg Asn Asp Gly Arg Ile Trp Ser Pro Lys Glu Pro Asn1 5 10 15gat aac cgc gat cca aac acc atc cct gag gat gag cgc gac tac ttc 96Asp Asn Arg Asp Pro Asn Thr Ile Pro Glu Asp Glu Arg Asp Tyr Phe
20 25 30ctg gag cgc cgc tac cca gca ttc ggt aac ctc gtc cca cgt gac gtt 144Leu Glu Arg Arg Tyr Pro Ala Phe Gly Asn Leu Val Pro Arg Asp Val
35 40 45gct tcc cgt gcg atc tcc cag cag atc aat gct ggt ctc ggt gtt gga 192Ala Ser Arg Ala Ile Ser Gln Gln Ile Asn Ala Gly Leu Gly Val Gly
50 55 60cct ctg aac aac gct gca tac ctg gac ttc cgc gac gcc acc gag cgc 240Pro Leu Asn Asn Ala Ala Tyr Leu Asp Phe Arg Asp Ala Thr Glu Arg65 70 75 80ctc gga cag gac acc atc cgc gag cgt tac tcc aac ctc ttc acc atg 288Leu Gly Gln Asp Thr Ile Arg Glu Arg Tyr Ser Asn Leu Phe Thr Met
85 90 95tac gaa gag gca att ggc gag gac cca tac tcc agc cca atg cgt att 336Tyr Glu Glu Ala Ile Gly Glu Asp Pro Tyr Ser Ser Pro Met Arg Ile
100 105 110gca ccg acc tgc cac ttc acc atg ggt ggc ctc tgg act gac ttc aac 384Ala Pro Thr Cys His Phe Thr Met Gly Gly Leu Trp Thr Asp Phe Asn
115 120 125gaa atg acg tca ctc cca ggt ctg ttc tgc gca ggc gaa gca tcc tgg 432Glu Met Thr Ser Leu Pro Gly Leu Phe Cys Ala Gly Glu Ala Ser Trp
130 135 140acc tac cac ggt gca aac cgt ctg ggc gca aac tcc ctg ctc tcc gct 480Thr Tyr His Gly Ala Asn Arg Leu Gly Ala Asn Ser Leu Leu Ser Ala145 150 155 160tcc gtc gat ggc tgg ttc acc ctg cca ttc acc atc cct aac tac ctc 528Ser Val Asp Gly Trp Phe Thr Leu Pro Phe Thr Ile Pro Ash Tyr Leu
165 170 175ggc cca ttg ctt ggc tcc gag cgt ctg tca gag gat gca cca gaa gca 576Gly Pro Leu Leu Gly Ser GIu Arg Leu Ser Glu Asp Ala Pro Glu Ala
180 185 190cag gca gcg att gcg cgt gca cag gct cgc att gac cgc ctc atg ggc 624Gln Ala Ala Ile Ala Arg Ala Gln Ala Arg Ile Asp Arg Leu Met Gly
195 200 205aac cgc cca gag tgg gtc ggt gac aac gtt cac gga cct gag tac tac 672Asn Arg Pro Glu Trp Val Gly Asp Asn Val His Gly Pro Glu Tyr Tyr
210 215 220cac cgc cag ctt ggc gat atc ctg tac ttc tcc tgt ggc gtt tcc cga 720His Arg Gln Leu Gly Asp Ile Leu Tyr Phe Ser Cys Gly Val Ser Arg225 230 235 240aac gta gaa gac ctc cag gat ggc atc aac aag atc cgt gcc ctc cgc 768Asn Val Glu Asp Leu Gln Asp Gly Ile Asn Lys Ile Arg Ala Leu Arg
245 250 255gat gac ttc tgg aag aac atg cgc atc acc ggc agc acc gat gag atg 816Asp Asp Phe Trp Lys Asn Met Arg Ile Thr Gly Ser Thr Asp Glu Met
260 265 270aac cag gtt ctc gaa tac gca gca cgc gta gcc gac tac atc gac ctc 864Asn Gln Val Leu Glu Tyr Ala Ala Arg Val Ala Asp Tyr Ile Asp Leu
275 280 285ggc gaa ctc atg tgt gtc gac gcc ctc gac cgc gac gag tcc tgt ggc 912Gly Glu Leu Met Cys Val Asp Ala Leu Asp Arg Asp Glu Ser Cys Gly
290 295 300gct cac ttc cgc gac gac cac ctc tcc gaa gat ggc gaa gca caa cgt 960Ala His Phe Arg Asp Asp His Leu Ser Glu Asp Gly Glu Ala Gln Arg305 310 315 320gac gac caa aac tgg tgc ttc gtc tcc gca tgg gaa cca ggc gag aat 1008Asp Asp Gln Asn Trp Cys Phe Val Ser Ala Trp Glu Pro Gly Glu Asn
325 330 335gga acc ttc gtc tgc cac gca gaa cca ctg ttc ttc gaa tct gtc cca 1056Gly Thr Phe Val Cys His Ala Glu Pro Leu Phe Phe Glu Ser Val Pro
340 345 350ctg cag aca agg aac tac aag taatgaaact tacacttgag atc 1100Leu Gln Thr Arg Asn Tyr Lys
355<210>12<211>359<212>PRT<213>谷氨酸棒杆菌<400>12Glu Ser Leu Arg Asn Asp Gly Arg Ile Trp Ser Pro Lys Glu Pro Asn1 5 10 15Asp Asn Arg Asp Pro Asn Thr Ile Pro Glu Asp Glu Arg Asp Tyr Phe
20 25 30Leu Glu Arg Arg Tyr Pro Ala Phe Gly Asn Leu Val Pro Arg Asp Val
35 40 45Ala Ser Arg Ala Ile Ser Gln Gln Ile Asn Ala Gly Leu Gly Val Gly
50 55 60Pro Leu Asn Asn Ala Ala Tyr Leu Asp Phe Arg Asp Ala Thr Glu Arg65 70 75 80Leu Gly Gln Asp Thr Ile Arg Glu Arg Tyr Ser Asn Leu Phe Thr Met
85 90 95Tyr Glu Glu Ala Ile Gly Glu Asp Pro Tyr Ser Ser Pro Met Arg Ile
100 105 110Ala Pro Thr Cys His Phe Thr Met Gly Gly Leu Trp Thr Asp Phe Asn
115 120 125Glu Met Thr Ser Leu Pro Gly Leu Phe Cys Ala Gly Glu Ala Ser Trp
130 135 140Thr Tyr His Gly Ala Asn Arg Leu Gly Ala Asn Ser Leu Leu Ser Ala145 150 155 160Ser Val Asp Gly Trp Phe Thr Leu Pro Phe Thr Ile Pro Asn Tyr Leu
165 170 175Gly Pro Leu Leu Gly Ser Glu Arg Leu Ser Glu Asp Ala Pro Glu Ala
180 185 190Gln Ala Ala Ile Ala Arg Ala Gln Ala Arg Ile Asp Arg Leu Met Gly
195 200 205Asn Arg Pro Glu Trp Val Gly Asp Asn Val His Gly Pro Glu Tyr Tyr
210 215 220His Arg Gln Leu Gly Asp Ile Leu Tyr Phe Ser Cys Gly Val Ser Arg225 230 235 240Asn Val Glu Asp Leu Gln Asp Gly Ile Asn Lys Ile Arg Ala Leu Arg
245 250 255Asp Asp Phe Trp Lys Asn Met Arg Ile Thr Gly Ser Thr Asp Glu Met
260 265 270Asn Gln Val Leu Glu Tyr Ala Ala Arg Val Ala Asp Tyr Ile Asp Leu
275 280 285Gly Glu Leu Met Cys Val Asp Ala Leu Asp Arg Asp Glu Ser Cys Gly
290 295 300Ala His Phe Arg Asp Asp His Leu Ser Glu Asp Gly Glu Ala Gln Arg305 310 316 320Asp Asp Gln Asn Trp Cys Phe Val Ser Ala Trp Glu Pro Gly Glu Asn
325 330 335Gly Thr Phe Val Cys His Ala Glu Pro Leu Phe Phe Glu Ser Val Pro
340 345 350Leu Gln Thr Arg Asn Tyr Lys
355<210>13<211>1593<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1570)<223>RXN00231<400>13caggactacc tcgacgccgg cgccaacttt gtccgagtcg gtgccgatgt ccagcaactc 60aacgctgctg gatacgaaaa gtgaaggaaa ataacgcatc atg act att aat gtt 115
Met Thr Ile Asn Val
1 5ttc gaa cta ctt gtc aaa agt ccc acg ggt cta ctg att ggt gat tcc 163Phe Glu Leu Leu Val Lys Ser Pro Thr Gly Leu Leu Ile Gly Asp Ser
10 15 20tgg gtg gaa gca tcc gac ggc ggt act ttc gat gtg gaa aac cca gcg 211Trp Val Glu Ala Ser Asp Gly Gly Thr Phe Asp Val Glu Asn Pro Ala
25 30 35acg ggt gaa aca atc gca acg ctc gcg tct gct act tcc gag gat gca 259Thr Gly Glu Thr Ile Ala Thr Leu Ala Ser Ala Thr Ser Glu Asp Ala
40 45 50ctg gct gct ctt gat gct gca tgc gct gtt cag gcc gag tgg gct agg 307Leu Ala Ala Leu Asp Ala Ala Cys Ala Val Gln Ala Glu Trp Ala Arg
55 60 65atg cca gcg cgc gag cgt tct aat att tta cgc cgc ggt ttt gag ctc 355Met Pro Ala Arg Glu Arg Ser Asn Ile Leu Arg Arg Gly Phe Glu Leu70 75 80 85gta gca gaa cgt gca gaa gag ttc gcc acc ctc atg acc ttg gaa atg 403Val Ala Glu Arg Ala Glu Glu Phe Ala Thr Leu Met Thr Leu Glu Met
90 95 100ggc aag cct ttg gct gaa gct cgc ggc gaa gtc acc tac ggc aac gaa 451Gly Lys Pro Leu Ala Glu Ala Arg Gly Glu Val Thr Tyr Gly Asn Glu
105 110 115ttc ctg cgc tgg ttc tct gag gaa gca gtt cgt ctg tat ggc cgt tac 499Phe Leu Arg Trp Phe Ser Glu Glu Ala Val Arg Leu Tyr Gly Arg Tyr
120 125 130gga acc aca cca gaa ggc aac ttg cgg atg ctg acc gcc ctc aag cca 547Gly Thr Thr Pro Glu Gly Asn Leu Arg Met Leu Thr Ala Leu Lys Pro
135 140 145gtt ggc ccg tgc ctc ctg atc acc cca tgg aac ttc cca cta gca atg 595Val Gly Pro Cys Leu Leu Ile Thr Pro Trp Asn Phe Pro Leu Ala Met150 155 160 165gct acc cgc aag gtc gca cct gcg atc gct gca ggt tgt gtc atg gtg 643Ala Thr Arg Lys Val Ala Pro Ala Ile Ala Ala Gly Cys Val Met Val
170 175 180ctc aag cca gct cga ctt acc ccg ctg acc tcc cag tat ttt gct cag 691Leu Lys Pro Ala Arg Leu Thr Pro Leu Thr Ser Gln Tyr Phe Ala Gln
185 190 195acc atg ctt gat gcc ggt ctt cca gca ggt gtc ctc aat gtg gtc tcc 739Thr Met Leu Asp Ala Gly Leu Pro Ala Gly Val Leu Asn Val Val Ser
200 205 210ggt gct tcc gcc tct gcg att tcc aac ccg att atg gaa gac gat cgc 787Gly Ala Ser Ala Ser Ala Ile Ser Asn Pro Ile Met Glu Asp Asp Arg
215 220 225ctt cgt aaa gtc tcc ttc acc ggc tcc acc cca gtt ggc cag cag ctg 835Leu Arg Lys Val Ser Phe Thr Gly Ser Thr Pro Val Gly Gln Gln Leu230 235 240 245ctc aaa aag gct gcc gat aaa gtt ctg cgc acc tcc atg gaa ctt ggt 883Leu Lys Lys Ala Ala Asp Lys Val Leu Arg Thr Ser Met Glu Leu Gly
250 255 260ggc aac gca cct ttc att gtc ttc gag gac gcc gac cta gat ctc gcg 931Gly Asn Ala Pro Phe Ile Val Phe Glu Asp Ala Asp Leu Asp Leu Ala
265 270 275atc gaa ggt gcc atg ggt gcc aaa atg cgc aac atc ggc gaa gct tgc 979Ile Glu Gly Ala Met Gly Ala Lys Met Arg Asn Ile Gly Glu Ala Cys
280 285 290acc gca gcc aac cgt ttc tta gtc cac gaa tcc gtc gcc gat gaa ttc 1027Thr Ala Ala Asn Arg Phe Leu Val His Glu Ser Val Ala Asp Glu Phe
295 300 305ggc cgt cgc ttc gct gcc cgc ctt gaa gag caa gtc cta ggc aac ggc 1075Gly Arg Arg Phe Ala Ala Arg Leu Glu Glu Gln Val Leu Gly Asn Gly310 315 320 325ctc gac gaa ggc gtc acc gtg ggc ccc ctg gtt gag gaa aaa gca cga 1123Leu Asp Glu Gly Val Thr Val Gly Pro Leu Val Glu Glu Lys Ala Arg
330 335 340gac agc gtt gca tcg ctt gtc gac gcc gcc gtc gcc gaa ggt gcc acc 1171Asp Ser Val Ala Ser Leu Val Asp Ala Ala Val Ala Glu Gly Ala Thr
345 350 355gtc ctc acc ggc ggc aag gcc ggc aca ggt gca ggc tac ttc tac gaa 1219Val Leu Thr Gly Gly Lys Ala Gly Thr Gly Ala Gly Tyr Phe Tyr Glu
360 365 370cca acg gtg ctc acg gga gtt tca aca gat gcg gct atc ctg aac gaa 1267Pro Thr Val Leu Thr Gly Val Ser Thr Asp Ala Ala Ile Leu Asn Glu
375 380 385gag atc ttc ggt ccc gtc gca ccg atc gtc acc ttc caa acc gag gaa 1315Glu Ile Phe Gly Pro Val Ala Pro Ile Val Thr Phe Gln Thr Glu Glu390 395 400 405gaa gcc ctg cgt cta gcc aac tcc acc gaa tac gga ctg gcc tcc tat 1363Glu Ala Leu Arg Leu Ala Asn Ser Thr Glu Tyr Gly Leu Ala Ser Tyr
410 415 420gtg ttc acc cag gac acc tca cgt att ttc cgc gtc tcc gat ggt ctc 1411Val Phe Thr Gln Asp Thr Ser Arg Ile Phe Arg Val Ser Asp Gly Leu
425 430 435gag ttc ggc cta gtg ggc gtc aat tcc ggt gtc atc tct aac gct gct 1459Glu Phe Gly Leu Val Gly Val Asn Ser Gly Val Ile Ser Asn Ala Ala
440 445 450gca cct ttt ggt ggc gta aaa caa tcc gga atg ggc cgc gaa ggt ggt 1507Ala Pro Phe Gly Gly Val Lys Gln Ser Gly Met Gly Arg Glu Gly Gly
455 460 165ctc gaa gga atc gag gag tac acc tcc gtg cag tac atc ggt atc cgg 1555Leu Glu Gly Ile Glu Glu Tyr Thr Ser Val Gln Tyr Ile Gly Ile Arg470 475 480 485gat cct tac gcc ggc tagcatctgc ccctttacaa atc 1593Asp Pro Tyr Ala Gly
490<210>14<211>490<212>PRT<213>谷氨酸棒杆菌<400>14Met Thr Ile Asn Val Phe Glu Leu Leu Val Lys Ser Pro Thr Gly Leu1 5 10 15Leu Ile Gly Asp Ser Trp Val Glu Ala Ser Asp Gly Gly Thr Phe Asp
20 25 30Val Glu Asn Pro Ala Thr Gly Glu Thr Ile Ala Thr Leu Ala Ser Ala
35 40 45Thr Ser Glu Asp Ala Leu Ala Ala Leu Asp Ala Ala Cys Ala Val Gln
50 55 60Ala Glu Trp Ala Arg Met Pro Ala Arg Glu Arg Ser Asn Ile Leu Arg65 70 75 80Arg Gly Phe Glu Leu Val Ala Glu Arg Ala Glu Glu Phe Ala Thr Leu
85 90 95Met Thr Leu Glu Met Gly Lys Pro Leu Ala Glu Ala Arg Gly Glu Val
100 105 110Thr Tyr Gly Asn Glu Phe Leu Arg Trp Phe Ser Glu Glu Ala Val Arg
115 120 125Leu Tyr Gly Arg Tyr Gly Thr Thr Pro Glu Gly Asn Leu Arg Met Leu
130 135 140Thr Ala Leu Lys Pro Val Gly Pro Cys Leu Leu Ile Thr Pro Trp Asn145 150 155 160Phe Pro Leu Ala Met Ala Thr Arg Lys Val Ala Pro Ala Ile Ala Ala
165 170 175Gly Cys Val Met Val Leu Lys Pro Ala Arg Leu Thr Pro Leu Thr Ser
180 185 190Gln Tyr Phe Ala Gln Thr Met Leu Asp Ala Gly Leu Pro Ala Gly Val
195 200 205Leu Asn Val Val Ser Gly Ala Ser Ala Ser Ala Ile Ser Asn Pro Ile
210 215 220Met Glu Asp Asp Arg Leu Arg Lys Val Ser Phe Thr Gly Ser Thr Pro225 230 235 240Val Gly Gln Gln Leu Leu Lys Lys Ala Ala Asp Lys Val Leu Arg Thr
245 250 255Ser Met Glu Leu Gly Gly Asn Ala Pro Phe Ile Val Phe Glu Asp Ala
260 265 270Asp Leu Asp Leu Ala Ile Glu Gly Ala Met Gly Ala Lys Met Arg Asn
275 280 285Ile Gly Glu Ala Cys Thr Ala Ala Asn Arg Phe Leu Val His Glu Ser
290 295 300Val Ala Asp Glu Phe Gly Arg Arg Phe Ala Ala Arg Leu Glu Glu Gln305 310 315 320Val Leu Gly Asn Gly Leu Asp Glu Gly Val Thr Val Gly Pro Leu Val
325 330 335Glu Glu Lys Ala Arg Asp Ser Val Ala Ser Leu Val Asp Ala Ala Val
340 345 350Ala Glu Gly Ala Thr Val Leu Thr Gly Gly Lys Ala Gly Thr Gly Ala
355 360 365Gly Tyr Phe Tyr Glu Pro Thr Val Leu Thr Gly Val Ser Thr Asp Ala
370 375 380Ala Ile Leu Asn Glu Glu Ile Phe Gly Pro Val Ala Pro Ile Val Thr385 390 395 400Phe Gln Thr Glu Glu Glu Ala Leu Arg Leu Ala Asn Ser Thr Glu Tyr
405 410 415Gly Leu Ala Ser Tyr Val Phe Thr Gln Asp Thr Ser Arg Ile Phe Arg
420 425 430Val Ser Asp Gly Leu Glu Phe Gly Leu Val Gly Val Asn Ser Gly Val
435 440 445Ile Ser Asn Ala Ala Ala Pro Phe Gly Gly Val Lys Gln Ser Gly Met
450 455 460Gly Arg Glu Gly Gly Leu Glu Gly Ile Glu Glu Tyr Thr Ser Val Gln465 470 475 480Tyr Ile Gly Ile Arg Asp Pro Tyr Ala Gly
485 490<210>15<211>870<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(847)<223>RXA01311<400>15tcgtctccgc atgggaacca ggcgagaatg gaaccttcgt ctgccacgca gaaccactgt 60tcttcgaatc tgtcccactg cagacaagga actacaagta atg aaa ctt aca ctt 115
Met Lys Leu Thr Leu
1 5gag atc tgg cgt caa gca ggc cca act gcg gaa ggc aag ttc gaa acc 163Glu Ile Trp Arg Gln Ala Gly Pro Thr Ala Glu Gly Lys Phe Glu Thr
10 15 20gtc cag gtt gac gac gcc gtc gcg cag atg tcc atc ctg gag ctg ctt 211Val Gln Val Asp Asp Ala Val Ala Gln Met Ser Ile Leu Glu Leu Leu
25 30 35gac cac gta aac aac aag ttc atc gaa gaa ggc aaa gaa cca ttc gcg 259Asp His Val Asn Asn Lys Phe Ile Glu Glu Gly Lys Glu Pro Phe Ala
40 45 50ttc gcc tct gac tgc cgc gaa ggc att tgt ggt acc tgt ggt ctc ctc 307Phe Ala Ser Asp Cys Arg Glu Gly Ile Cys Gly Thr Cys Gly Leu Leu
55 60 65gtg aac ggt cgc cct cac ggc gcc gac cag aac aag cct gcc tgt gcg 355Val Asn Gly Arg Pro His Gly Ala Asp Gln Asn Lys Pro Ala Cys Ala70 75 80 85cag cgc ctg gtc agc tac aag gaa ggc gac acc ctc aag atc gaa cca 403Gln Arg Leu Val Ser Tyr Lys Glu Gly Asp Thr Leu Lys Ile Glu Pro
90 95 100ctg cgt tcc gcc gca tac cca gtg atc aag gac atg gtc gtc gac cgc 451Leu Arg Ser Ala Ala Tyr Pro Val Ile Lys Asp Met Val Val Asp Arg
105 110 115tcc gca ctg gac cgt gtc atg gaa cag ggt ggc tac gtg acc atc aac 499Ser Ala Leu Asp Arg Val Met Glu Gln Gly Gly Tyr Val Thr Ile Asn
120 125 130gca ggt acc gca cct gac gct gat acc ctc cac gtc aac cac gaa acc 547Ala Gly Thr Ala Pro Asp Ala Asp Thr Leu His Val Asn His Glu Thr
135 140 145gca gaa ctc gca ctt gac cac gca gcc tgc atc ggc tgt ggc gca tgt 595Ala Glu Leu Ala Leu Asp His Ala Ala Cys Ile Gly Cys Gly Ala Cys150 155 160 165gtt gct gcc tgc cct aac ggc gca gca cac ctg ttc acc ggc gca aag 643Val Ala Ala Cys Pro Asn Gly Ala Ala His Leu Phe Thr Gly Ala Lys
170 175 180ctt gtt cac ctc tcc ctc ctc cca ctg ggt aag gaa gag cgc gga ctg 691Leu Val His Leu Ser Leu Leu Pro Leu Gly Lys Glu Glu Arg Gly Leu
185 190 195cgt gca cgt aag atg gtt gat gaa atg gaa acc aac ttc gga cac tgc 739Arg Ala Arg Lys Met Val Asp Glu Met Glu Thr Asn Phe Gly His Cys
200 205 210tcc ctc tac ggc gag tgc gca gat gtc tgc ccc gca ggc atc cca ctg 787Ser Leu Tyr Gly Glu Cys Ala Asp Val Cys Pro Ala Gly Ile Pro Leu
215 220 225acc gct gtg gca gct gtc acc aag gaa cgt gcg cgt gca gct ttc cga 835Thr Ala Val Ala Ala Val Thr Lys Glu Arg Ala Arg Ala Ala Phe Arg230 235 240 245ggc aaa gac gac tagtctttaa tccaagtaag tac 870Gly Lys Asp Asp<210>16<211>249<212>PRT<213>谷氨酸棒杆菌<400>16Met Lys Leu Thr Leu Glu Ile Trp Arg Gln Ala Gly Pro Thr Ala Glu1 5 10 15Gly Lys Phe Glu Thr Val Gln Val Asp Asp Ala Val Ala Gln Met Ser
20 25 30Ile Leu Glu Leu Leu Asp His Val Asn Asn Lys Phe Ile Glu Glu Gly
35 40 45Lys Glu Pro Phe Ala Phe Ala Ser Asp Cys Arg Glu Gly Ile Cys Gly
50 55 60Thr Cys Gly Leu Leu Val Asn Gly Arg Pro His Gly Ala Asp Gln Asn65 70 75 80Lys Pro Ala Cys Ala Gln Arg Leu Val Ser Tyr LysGlu Gly Asp Thr
85 90 95Leu Lys Ile Glu Pro Leu Arg Ser Ala Ala Tyr Pro Val Ile Lys Asp
100 105 110Met Val Val Asp Arg Ser Ala Leu Asp Arg Val Met Glu Gln Gly Gly
115 120 125Tyr Val Thr Ile Asn Ala Gly Thr Ala Pro Asp Ala Asp Thr Leu His
130 135 140Val Asn His Glu Thr Ala Glu Leu Ala Leu Asp His Ala Ala Cys Ile145 150 155 160Gly Cys Gly Ala Cys Val Ala Ala Cys Pro Asn Gly Ala Ala His Leu
165 170 175Phe Thr Gly Ala Lys Leu Val His Leu Ser Leu Leu Pro Leu Gly Lys
180 185 190Glu Glu Arg Gly Leu Arg Ala Arg Lys Met Val Asp Glu Met Glu Thr
195 200 205Asn Phe Gly His Cys Ser Leu Tyr Gly Glu Cys Ala Asp Val Cys Pro
210 215 220Ala Gly Ile Pro Leu Thr Ala Val Ala Ala Val Thr Lys Glu Arg Ala225 230 235 240Arg Ala Ala Phe Arg Gly Lys Asp Asp
245<210>17<211>1530<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1507)<223>RXA01535<400>17acccacctca ctctaggggt ggactccagt gtttcgcgac aacacaatga gtaagcttgt 60gacagccgta tttaattctc agtaagaaat gagtgatttc atg acc gag cag gaa 115
Met Thr Glu Gln Glu
1 5ttc cgt att gag cac gac acc atg ggt gaa gtg aag gtt cca gca aag 163Phe Arg Ile Glu His Asp Thr Met Gly Glu Val Lys Val Pro Ala Lys
10 15 20gct ctg tgg cag gca cag acc cag cgc gct gtt gag aac ttc cct atc 211Ala Leu Trp Gln Ala Gln Thr Gln Arg Ala Val Glu Asn Phe Pro Ile
25 30 35tct ggt cgt ggt ctg gaa tcc gca cag atc cgc gca atg ggt ctg ctg 259Ser Gly Arg Gly Leu Glu Ser Ala Gln Ile Arg Ala Met Gly Leu Leu
40 45 50aag gca gct tgt gcg cag gta aac aag gac tcc ggt gcg ctg gat gca 307Lys Ala Ala Cys Ala Gln Val Asn Lys Asp Ser Gly Ala Leu Asp Ala
55 60 65gag aag gca gat gcc atc att gca gct ggt aag gag atc gcg tcc ggt 355Glu Lys Ala Asp Ala Ile Ile Ala Ala Gly Lys Glu Ile Ala Ser Gly70 75 80 85aag cat gac gct gag ttc cca att gat gtg ttc cag act ggt tcc ggt 403Lys His Asp Ala Glu Phe Pro Ile Asp Val Phe Gln Thr Gly Ser Gly
90 95 100act tcc tcc aac atg aac acc aat gag gtt atc gct tcc atc gcg aag 451Thr Ser Ser Asn Met Asn Thr Asn Glu Val Ile Ala Ser Ile Ala Lys
105 110 115gct aac ggc gtt gag gtt cac cca aat gac cac gtc aac atg ggt cag 499Ala Asn Gly Val Glu Val His Pro Asn Asp His Val Asn Met Gly Gln
120 125 130tcc tcc aat gac acc ttc cct act gca act cac gtt gct gca acc gaa 547Ser Ser Asn Asp Thr Phe Pro Thr Ala Thr His Val Ala Ala Thr Glu
135 140 145gct gct gtc aat gac ctc atc cca ggc ctg aag gtt ctg cac gag tct 595Ala Ala Val Asn Asp Leu Ile Pro Gly Leu Lys Val Leu His Glu Ser150 155 160 165ttg gcg aag aag gct aac gag tgg tct gag gtt gtt aag tcc ggc cgc 643Leu Ala Lys Lys Ala Asn Glu Trp Ser Glu Val Val Lys Ser Gly Arg
170 175 180acc cac ctg atg gac gct gtt cca gta acc ctg ggc cag gag ttc ggt 691Thr His Leu Met Asp Ala Val Pro Val Thr Leu Gly Gln Glu Phe Gly
185 190 195ggc tac gct cgc cag atc cag ctc ggc atc gag cgc gtt gag gct act 739Gly Tyr Ala Arg Gln Ile Gln Leu Gly Ile Glu Arg Val Glu Ala Thr
200 205 210ctt cct cgc ctt ggt gag ctg gct att ggt ggc acc gct gct ggt acc 787Leu Pro Arg Leu Gly Glu Leu Ala Ile Gly Gly Thr Ala Ala Gly Thr
215 220 225ggt atc aac acc tcc gct gat ttc ggc ggc aag gtt gtt gct gaa ctg 835Gly Ile Asn Thr Ser Ala Asp Phe Gly Gly Lys Val Val Ala Glu Leu230 235 240 245atc aac ttg acc gac gtc aag gag ctc aag gaa gct gag aac cac ttc 883Ile Asn Leu Thr Asp Val Lys Glu Leu Lys Glu Ala Glu Asn His Phe
250 255 260gag gct cag gct gca cgc gac gct ctt gtt gag ttc tcc ggc gca atg 931Glu Ala Gln Ala Ala Arg Asp Ala Leu Val Glu Phe Ser Gly Ala Met
265 270 275cgc gtt atc gct gtc tcc ttg tac aag atc gct aac gat atc cgc ctc 979Arg Val Ile Ala Val Ser Leu Tyr Lys Ile Ala Asn Asp Ile Arg Leu
280 285 290atg ggc tcc ggc cca ctg acc ggt ctt ggc gag atc cgt ctc cca gac 1027Met Gly Ser Gly Pro Leu Thr Gly Leu Gly Glu Ile Arg Leu Pro Asp
295 300 305ctg cag cca ggt tcc tcc arc atg cca ggc aag gtc aac cca gtt crc 1075Leu Gln Pro Gly Ser Ser Ile Met Pro Gly Lys Val Asn Pro Val Leu310 315 320 325tgt gag acc gct acc cag gtt tcc gct cag gtt atc ggc aat gac gca 1123Cys Glu Thr Ala Thr Gln Val Ser Ala Gln Val Ile Gly Asn Asp Ala
330 335 340gct gtt gcg ttc tcc ggc acc cag ggc cag ttc gag ctc aac gtg ttc 1171Ala Val Ala Phe Ser Gly Thr Gln Gly Gln Phe Glu Leu Asn Val Phe
345 350 355atc cca gtg atg gct cgc aac gtg ctt gag tcc gct cgc ctg ctg gct 1219Ile Pro Val Met Ala Arg Asn Val Leu Glu Set Ala Arg Leu Leu Ala
360 365 370aac act tcc cgc gtg ttc gca acc cgt ctc gtt gat ggc att gag cca 1267Asn Thr Set Arg Val Phe Ala Thr Arg Leu Val Asp Gly Ile Glu Pro
375 380 385aac gag gca cac atg aag gag ctc gct gag tct tca cct tcc atc gtt 1315Asn Glu Ala His Met Lys Glu Leu Ala Glu Ser Ser Pro Ser Ile Val390 395 400 405acc cca ctg aac tct gca atc ggc tac gaa gct gct gca aag gtg gct 1363Thr Pro Leu Asn Ser Ala Ile Gly Tyr Glu Ala Ala Ala Lys Val Ala
410 415 420aag act gct ttg gct gag ggc aag acc atc cgc cag act gtc atc gat 1411Lys Thr Ala Leu Ala Glu Gly Lys Thr Ile Arg Gln Thr Val Ile Asp
425 430 435ttg ggc ttg gtt gat ggc gag aag ctc acc gag gaa gag ctg gac aag 1459Leu Gly Leu Val Asp Gly Glu Lys Leu Thr Glu Glu Glu Leu Asp Lys
440 445 450cgc ctc gac gtt ctt gct atg gct cac acc gag cgc gag aac aag ttc 1507Arg Leu Asp Val Leu Ala Met Ala His Thr Glu Arg Glu Asn Lys Phe
455 460 465taaaactaga acccgataaa taa 1530<210>18<211>469<212>PRT<213>谷氨酸棒杆菌<400>18Met Thr Glu Gln Glu Phe Arg Ile Glu His Asp Thr Met Gly Glu Val1 5 10 15Lys Val Pro Ala Lys Ala Leu Trp Gln Ala Gln Thr Gln Arg Ala Val
20 25 30Glu Asn Phe Pro Ile Ser Gly Arg Gly Leu Glu Ser Ala Gln Ile Arg
35 40 45Ala Met Gly Leu Leu Lys Ala Ala Cys Ala Gln Val Asn Lys Asp Ser
50 55 60Gly Ala Leu Asp Ala Glu Lys Ala Asp Ala Ile Ile Ala Ala Gly Lys65 70 75 80Glu Ile Ala Ser Gly Lys His Asp Ala Glu Phe Pro Ile Asp Val Phe
85 90 95Gln Thr Gly Ser Gly Thr Ser Ser Asn Met Asn Thr Asn Glu Val Ile
100 105 110Ala Ser Ile Ala Lys Ala Asn Gly Val Glu Val His Pro Asn Asp His
115 120 125Val Asn Met Gly Gln Ser Ser Asn Asp Thr Phe Pro Thr Ala Thr His
130 135 140Val Ala Ala Thr Glu Ala Ala Val Asn Asp Leu Ile Pro Gly Leu Lys145 150 155 160Val Leu His Glu Ser Leu Ala Lys Lys Ala Asn Glu Trp Ser Glu Val
165 170 175Val Lys Ser Gly Arg Thr His Leu Met Asp Ala Val Pro Val Thr Leu
180 185 190Gly Gln Glu Phe Gly Gly Tyr Ala Arg Gln Ile Gln Leu Gly Ile Glu
195 200 205Arg Val Glu Ala Thr Leu Pro Arg Leu Gly Glu Leu Ala Ile Gly Gly
210 215 220Thr Ala Ala Gly Thr Gly Ile Asn Thr Ser Ala Asp Phe Gly Gly Lys225 230 235 240Val Val Ala Glu Leu Ile Asn Leu Thr Asp Val Lys Glu Leu Lys Glu
245 250 255Ala Glu Asn His Phe Glu Ala Gln Ala Ala Arg Asp Ala Leu Val Glu
260 265 270Phe Ser Gly Ala Met Arg Val Ile Ala Val Ser Leu Tyr Lys Ile Ala
275 280 285Asn Asp Ile Arg Leu Met Gly Ser Gly Pro Leu Thr Gly Leu Gly Glu
290 295 300Ile Arg Leu Pro Asp Leu Gln Pro Gly Ser Ser Ile Met Pro Gly Lys305 310 315 320Val Asn Pro Val Leu Cys Glu Thr Ala Thr Gln Val Ser Ala Gln Val
325 330 335Ile Gly Asn Asp Ala Ala Val Ala Phe Ser Gly Thr Gln Gly Gln Phe
340 345 350Glu Leu Asn Val Phe Ile Pro Val Met Ala Arg Asn Val Leu Glu Ser
355 360 365Ala Arg Leu Leu Ala Asn Thr Ser Arg Val Phe Ala Thr Arg Leu Val
370 375 380Asp Gly Ile Glu Pro Asn Glu Ala His Met Lys Glu Leu Ala Glu Ser385 390 395 400Ser Pro Ser Ile Val Thr Pro Leu Asn Ser Ala Ile Gly Tyr Glu Ala
405 410 415Ala Ala Lys Val Ala Lys Thr Ala Leu Ala Glu Gly Lys Thr Ile Arg
420 425 430Gln Thr Val Ile Asp Leu Gly Leu Val Asp Gly Glu Lys Leu Thr Glu
435 440 445Glu Glu Leu Asp Lys Arg Leu Asp Val Leu Ala Met Ala His Thr Glu
450 455 460Arg Glu Asn Lys Phe465<2l0>19<211>1164<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1141)<223>RXA00517<400>19ggtcttagaa ccagcgtgca ctgatggcga ttaaaggggg ttgcgcctat acctattgct 60ggtatacatt tcggtatacc taaaccgaat tgagggattc atg cca gaa gtc act 115
Met Pro Glu Val Thr
1 5gtc aac gcc caa caa ctc act gtt ctc tgc aca gac atc ctc acc aaa 163Val Asn Ala Gln Gln Leu Thr Val Leu Cys Thr Asp Ile Leu Thr Lys
10 15 20act gga gta cct gca gca gac gcc cat ctt gtc ggt gat agt ttg gtg 211Thr Gly Val Pro Ala Ala Asp Ala His Leu Val Gly Asp Ser Leu Val
25 30 35cag gct gat ctt tgg ggt cac ccc tcc cac ggt gtg ctt cga ctg cct 259Gln Ala Asp Leu Trp Gly His Pro Ser His Gly Val Leu Arg Leu Pro
40 45 50tgg tat gtg cgc aga ctc cac agt ggc gcg atg act aca cat gca cac 307Trp Tyr Val Arg Arg Leu His Ser Gly Ala Met Thr Thr His Ala His
55 60 65gtg gag gtt ctc aat gat ttg ggt gcc gtg ttg gcg ttg gat gga cac 355Val Glu Val Leu Asn Asp Leu Gly Ala Val Leu Ala Leu Asp Gly His70 75 80 85aat gga atc ggc caa gtt tta gct gat cat gct cgt aaa gaa gca gtg 403Asn Gly Ile Gly Gln Val Leu Ala Asp His Ala Arg Lys Glu Ala Val
90 95 100act agg gca atg atg ttc ggc atc ggt gcg gtg tcg gtg cgc aac tcc 451Thr Arg Ala Met Met Phe Gly Ile Gly Ala Val Ser Val Arg Asn Ser
105 110 115aat cat ttt gga act gcc atg tac tac acc cgg aaa gcg gca gcg caa 499Asn His Phe Gly Thr Ala Met Tyr Tyr Thr Arg Lys Ala Ala Ala Gln
120 125 130gga tgt gtt tcc att ctc acc acc aat gca tct ccg gcg atg gcg ccc 547Gly Cys Val Ser Ile Leu Thr Thr Asn Ala Ser Pro Ala Met Ala Pro
135 140 145tgg ggt ggc aga gaa aaa cgg atc ggt acc aac cca tgg tct att gcg 595Trp Gly Gly Arg Glu Lys Arg Ile Gly Thr Asn Pro Trp Ser Ile Ala150 155 160 165gca cct ttt gga gaa acg gct acg gta gtc gat ata gcc aat act gcg 643Ala Pro Phe Gly Glu Thr Ala Thr Val Val Asp Ile Ala Asn Thr Ala
170 175 180gtt gcg cgc gga aag atc tac cac gca cga cag aca aac atg ccc att 691Val Ala Arg Gly Lys Ile Tyr His Ala Arg Gln Thr Asn Met Pro Ile
185 190 195cct gag act tgg gcg atc acg agt gag ggc gca ccc acc acg gat cct 739Pro Glu Thr Trp Ala Ile Thr Ser Glu Gly Ala Pro Thr Thr Asp Pro
200 205 210gct gag gca atc aac ggt gtc gtc ctt ccc atg gct ggt cac aaa ggt 787Ala Glu Ala Ile Asn Gly Val Val Leu Pro Met Ala Gly His Lys Gly
215 220 225tat gcg att agc ttc atg atg gat gtg ctt tct gga gtt ctc act ggt 835Tyr Ala Ile Ser Phe Met Met Asp Val Leu Ser Gly Val Leu Thr Gly230 235 240 245tcc cag cac agc acc aag gta cat ggt ccg tat gat ccc act ccc cca 883Ser Gln His Ser Thr Lys Val His Gly Pro Tyr Asp Pro Thr Pro Pro
250 255 260ggt gga gct ggc cac ttg ttc atc gcg ttg gat gtt gca gcg ttt cgc 931Gly Gly Ala Gly His Leu Phe Ile Ala Leu Asp Val Ala Ala Phe Arg
265 270 275gat cca caa gat ttc gat gac gca ctc agc gat ctg gtt ggg gaa gtt 979Asp Pro Gln Asp Phe Asp Asp Ala Leu Ser Asp Leu Val Gly Glu Val
280 285 290aaa tcc act ccg aaa gca caa aac acc gag gag att ttc tac ccc ggt 1027Lys Ser Thr Pro Lys Ala Gln Asn Thr Glu Glu Ile Phe Tyr Pro Gly
295 300 305gaa tcg gaa gac cgt gcg cat cgg aaa aac tct gcg cac ggt att tca 1075Glu Ser Glu Asp Arg Ala His Arg Lys Asn Ser Ala His Gly Ile Ser310 315 320 325ttg cct gaa aaa acg tgg atg gaa ctg caa gaa ctg gca atc gag aac 1123Leu Pro Glu Lys Thr Trp Met Glu Leu Gln Glu Leu Ala Ile Glu Asn
330 335 340cat gtt gta act cac cgt tgatctgcgc gttaaacctg gcc 1164His Val Val Thr His Arg
345<210>20<211>347<212>PRT<213>谷氨酸棒杆菌<400>20Met Pro Glu Val Thr Val Asn Ala Gln Gln Leu Thr Val Leu Cys Thr1 5 10 15Asp Ile Leu Thr Lys Thr Gly Val Pro Ala Ala Asp Ala His Leu Val
20 25 30Gly Asp Ser Leu Val Gln Ala Asp Leu Trp Gly His Pro Ser His Gly
35 40 45Val Leu Arg Leu Pro Trp Tyr Val Arg Arg Leu His Ser Gly Ala Met
50 55 60Thr Thr His Ala His Val Glu Val Leu Asn Asp Leu Gly Ala Val Leu65 70 75 80Ala Leu Asp Gly His Asn Gly Ile Gly Gln Val Leu Ala Asp His Ala
85 90 95Arg Lys Glu Ala Val Thr Arg Ala Met Met Phe Gly Ile Gly Ala Val
100 105 110Ser Val Arg Asn Ser Asn His Phe Gly Thr Ala Met Tyr Tyr Thr Arg
115 120 125Lys Ala Ala Ala Gln Gly Cys Val Ser Ile Leu Thr Thr Asn Ala Ser
130 135 140Pro Ala Met Ala Pro Trp Gly Gly Arg Glu Lys Arg Ile Gly Thr Asn145 150 155 160Pro Trp Ser Ile Ala Ala Pro Phe Gly Glu Thr Ala Thr Val Val Asp
165 170 175Ile Ala Asn Thr Ala Val Ala Arg Gly Lys Ile Tyr His Ala Arg Gln
180 185 190Thr Asn Met Pro Ile Pro Glu Thr Trp Ala Ile Thr Ser Glu Gly Ala
195 200 205Pro Thr Thr Asp Pro Ala Glu Ala Ile Asn Gly Val Val Leu Pro Met
210 215 220Ala Gly His Lys Gly Tyr Ala Ile Ser Phe Met Met Asp Val Leu Ser225 230 235 240Gly Val Leu Thr Gly Ser Gln His Ser Thr Lys Val His Gly Pro Tyr
245 250 255Asp Pro Thr Pro Pro Gly Gly Ala Gly His Leu Phe Ile Ala Leu Asp
260 265 270Val Ala Ala Phe Arg Asp Pro Gln Asp Phe Asp Asp Ala Leu Ser Asp
275 280 285Leu Val Gly Glu Val Lys Ser Thr Pro Lys Ala Gln Asn Thr Glu Glu
290 295 300Ile Phe Tyr Pro Gly Glu Ser Glu Asp Arg Ala His Arg Lys Asn Ser305 310 315 320Ala His Gly Ile Ser Leu Pro Glu Lys Thr Trp Met Glu Leu Gln Glu
325 330 335Leu Ala Ile Glu Asn His Val Val Thr His Arg
340 345<210>21<211>1107<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1084)<223>RXA01350<400>21tgcagtatcg tcaagatcac ccaaaactgg tggctgttct cttttaagcg ggatagcatg 60ggttcttaga ggacccccta caaggattga ggattgttta atg aat tcc ccg cag 115
Met Asn Ser Pro Gln
1 5aac gtc tcc acc aag aag gtc acc gtc acc ggc gca gct ggt caa atc 163Asn Val Ser Thr Lys Lys Val Thr Val Thr Gly Ala Ala Gly Gln Ile
10 15 20tct tat tca ctg ttg tgg cgc atc gcc aac ggt gaa gta ttc ggc acc 211Ser Tyr Ser Leu Leu Trp Arg Ile Ala Asn Gly Glu Val Phe Gly Thr
25 30 35gac acc cct gta gaa ctg aaa ctt ctg gag atc cct cag gct ctt ggc 259Asp Thr Pro Val Glu Leu Lys Leu Leu Glu Ile Pro Gln Ala Leu Gly
40 45 50ggg gca gag ggt gtg gct atg gaa ctt ctg gat tct gcc ttc ccc ctc 307Gly Ala Glu Gly Val Ala Met Glu Leu Leu Asp Ser Ala Phe Pro Leu
55 60 65ctg cga aac atc acc atc acc gcg gat gcc aat gag gca ttc gac ggc 355Leu Arg Asn Ile Thr Ile Thr Ala Asp Ala Asn Glu Ala Phe Asp Gly70 75 80 85gct aat gcg gcg ttt ttg gtc ggt gcg aag cct cgc gga aaa ggc gaa 403Ala Asn Ala Ala Phe Leu Val Gly Ala Lys Pro Arg Gly Lys Gly Glu
90 95 100gag cgc gca gat ttg ctg gct aac aac ggc aag att ttc gga cct caa 451Glu Arg Ala Asp Leu Leu Ala Asn Asn Gly Lys Ile Phe Gly Pro Gln
105 110 115ggt aaa gct atc aat gac aac gcc gca gat gac att cgt gtc cta gtt 499Gly Lys Ala Ile Asn Asp Asn Ala Ala Asp Asp Ile Arg Val Leu Val
120 125 130gtt gga aac cca gcg aac acc aac gcg ttg att gct tca gct gcg gcc 547Val Gly Asn Pro Ala Asn Thr Asn Ala Leu Ile Ala Ser Ala Ala Ala
135 140 145cca gat gtt cca gca tcc cgc ttc aac gca atg atg cgc ctt gat cac 595Pro Asp Val Pro Ala Ser Arg Phe Asn Ala Met Met Arg Leu Asp His150 155 160 165aac cgt gcg atc tcc cag ctg gcc acc aag ctt ggc cgt gga tct gcg 643Asn Arg Ala Ile Ser Gln Leu Ala Thr Lys Leu Gly Arg Gly Ser Ala
170 175 180gaa ttt aac aac att gtg gtc tgg gga aat cac tcc gca acc cag ttc 691Glu Phe Asn Asn Ile Val Val Trp Gly Asn His Ser Ala Thr Gln Phe
185 190 195cca gac atc acc tac gca acc gtt ggt gga gaa aag gtc act gac ctg 739Pro Asp Ile Thr Tyr Ala Thr Val Gly Gly Glu Lys Val Thr Asp Leu
200 205 210gtt gat cac gat tgg tat gtg gag gag ttc att cct cgc gtg gct aac 787Val Asp His Asp Trp Tyr Val Glu Glu Phe Ile Pro Arg Val Ala Asn
215 220 225cgt ggc gct gaa atc att gag gtc cgt gga aag tct tct gca gct tct 835Arg Gly Ala Glu Ile Ile Glu Val Arg Gly Lys Ser Ser Ala Ala Ser230 235 240 245gca gca tcc tct gcg att gat cac atg cgc gat tgg gta cag ggc acc 883Ala Ala Ser Ser Ala Ile Asp His Met Arg Asp Trp Val Gln Gly Thr
250 255 260gag gcg tgg tcc tct gcg gca att cct tcc acc ggt gca tac ggc att 931Glu Ala Trp Ser Ser Ala Ala Ile Pro Ser Thr Gly Ala Tyr Gly Ile
265 270 275cct gag ggc att ttt gtc ggt ctg cca acc gta tcc cgc aac ggt gag 979Pro Glu Gly Ile Phe Val Gly Leu Pro Thr Val Ser Arg Asn Gly Glu
280 285 290tgg gaa atc gtt gaa ggc ctg gag att tcc gat ttc cag cgc gcc cgc 1027Trp Glu Ile Val Glu Gly Leu Glu Ile Ser Asp Phe Gln Arg Ala Arg
295 300 305atc gac gcg aat gct cag gaa ttg cag gcc gag cgc gag gca gtg cgc 1075Ile Asp Ala Asn Ala Gln Glu Leu Gln Ala Glu Arg Glu Ala Val Arg310 315 320 325gac ttg ctc taatctttaa cgcatgactt cgc 1107Asp Leu Leu<210>22<211>328<212>PRT<213>谷氨酸棒杆菌<400>22Met Asn Ser Pro Gln Asn Val Ser Thr Lys Lys Val Thr Val Thr Gly1 5 10 15Ala Ala Gly Gln Ile Ser Tyr Ser Leu Leu Trp Arg Ile Ala Asn Gly
20 25 30Glu Val Phe Gly Thr Asp Thr Pro Val Glu Leu Lys Leu Leu Glu Ile
35 40 45Pro Gln Ala Leu Gly Gly Ala Glu Gly Val Ala Met Glu Leu Leu Asp
50 55 60Ser Ala Phe Pro Leu Leu Arg Asn Ile Thr Ile Thr Ala Asp Ala Asn65 70 75 80Glu Ala Phe Asp Gly Ala Asn Ala Ala Phe Leu Val Gly Ala Lys Pro
85 90 95Arg Gly Lys Gly Glu Glu Arg Ala Asp Leu Leu Ala Asn Asn Gly Lys
100 105 110Ile Phe Gly Pro Gln Gly Lys Ala Ile Asn Asp Asn Ala Ala Asp Asp
115 120 125Ile Arg Val Leu Val Val Gly Asn Pro Ala Asn Thr Asn Ala Leu Ile
130 135 140Ala Ser Ala Ala Ala Pro Asp Val Pro Ala Ser Arg Phe Asn Ala Met145 150 155 160Met Arg Leu Asp His Asn Arg Ala Ile Ser Gln Leu Ala Thr Lys Leu
165 170 175Gly Arg Gly Ser Ala Glu Phe Asn Asn Ile Val Val Trp Gly Asn His
180 185 190Ser Ala Thr Gln Phe Pro Asp Ile Thr Tyr Ala Thr Val Gly Gly Glu
195 200 205Lys Val Thr Asp Leu Val Asp His Asp Trp Tyr Val Glu Glu Phe Ile
210 215 220Pro Arg Val Ala Asn Arg Gly Ala Glu Ile Ile Glu Val Arg Gly Lys225 230 235 240Ser Ser Ala Ala Ser Ala Ala Ser Ser Ala Ile Asp His Met Arg Asp
245 250 255Trp Val Gln Gly Thr Glu Ala Trp Ser Ser Ala Ala Ile Pro Ser Thr
260 265 270Gly Ala Tyr Gly Ile Pro Glu Gly Ile Phe Val Gly Leu Pro Thr Val
275 280 285Ser Arg Asn Gly Glu Trp Glu Ile Val Glu Gly Leu Glu Ile Ser Asp
290 295 300Phe Gln Arg Ala Arg Ile Asp Ala Asn Ala Gln Glu Leu Gln Ala Glu305 310 315 320Arg Glu Ala Val Arg Asp Leu Leu
325<210>23<211>1092<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1069)<223>RXA02149<400>23ttgcagagtg aaccacgatg atggttggac agctgttgat agctaatctt tgaaagatta 60aattcaccta aatcctgtgt agaacgcgag gggcactctt atg cca caa aaa ccg 115
Met Pro Gln Lys Pro
1 5gcc agt ttc gcg gtg ggc ttt gac atc ggc ggc acc aac atg cga gcc 163Ala Ser Phe Ala Val Gly Phe Asp Ile Gly Gly Thr Asn Met Arg Ala
10 15 20ggg ctt gtc gac gaa tcc ggg cgc atc gtg acc agt ttg tcg gcg ccg 211Gly Leu Val Asp Glu Ser Gly Arg Ile Val Thr Ser Leu Ser Ala Pro
25 30 35tcg ccg cgc acg acg cag gca atg gaa cag ggg att ttt gat cta gtc 259Ser Pro Arg Thr Thr Gln Ala Met Glu Gln Gly Ile Phe Asp Leu Val
40 45 50gaa cag ctc aag gcc gaa tac ccg gtt ggt gct gtg gga ctt gcc gtc 307Glu Gln Leu Lys Ala Glu Tyr Pro Val Gly Ala Val Gly Leu Ala Val
55 60 65gcg gga ttt ttg gat cct gag tgc gag gtt gtt cga ttt gcc ccg cac 355Ala Gly Phe Leu Asp Pro Glu Cys Glu Val Val Arg Phe Ala Pro His70 75 80 85ctt cct tgg cgc gat gag cca gtg cgt gaa aag ttg gaa aac ctt ttg 403Leu Pro Trp Arg Asp Glu Pro Val Arg Glu Lys Leu Glu Asn Leu Leu
90 95 100ggc ctg cct gtt cgt ttg gaa cat gat gcc aac tca gca gcg tgg ggt 451Gly Leu Pro Val Arg Leu Glu His Asp Ala Asn Ser Ala Ala Trp Gly
105 110 115gag cat cgt ttt ggt gca gct caa ggc gct gac aac tgg gtt ttg ttg 499Glu His Arg Phe Gly Ala Ala Gln Gly Ala Asp Asn Trp Val Leu Leu
120 125 130gca ctc ggc act gga att ggt gca gcg ctg att gaa aaa ggc gaa att 547Ala Leu Gly Thr Gly Ile Gly Ala Ala Leu Ile Glu Lys Gly Glu Ile
135 140 145tac cgt ggt gca tat ggc acg gca cca gaa ttt ggt cat ttg cgt gtt 595Tyr Arg Gly Ala Tyr Gly Thr Ala Pro Glu Phe Gly His Leu Arg Val150 155 160 165gtt cgt ggc gga cgc gca tgt gcg tgt ggc aaa gaa ggc tgc ctg gag 643Val Arg Gly Gly Arg Ala Cys Ala Cys Gly Lys Glu Gly Cys Leu Glu
170 175 180cgt tac tgt tcc ggt act gcc ttg gtt tac act gcg cgt gaa ttg gct 691Arg Tyr Cys Ser Gly Thr Ala Leu Val Tyr Thr Ala Arg Glu Leu Ala
185 190 195tcg cat ggc tca ttc cgc aac agc ggg ctg ttt gac aag atc aaa gcc 739Ser His Gly Ser Phe Arg Asn Ser Gly Leu Phe Asp Lys Ile Lys Ala
200 205 210gat ccg aat tcc atc aat gga aaa acg atc act gcg gca gcg cgc caa 787Asp Pro Asn Ser Ile Asn Gly Lys Thr Ile Thr Ala Ala Ala Arg Gln
215 220 225gaa gac cca ctt gct ctc gcc gtt ctg gaa gat ttc agc gag tgg ctg 835Glu Asp Pro Leu Ala Leu Ala Val Leu Glu Asp Phe Ser Glu Trp Leu230 235 240 245ggc gaa act ttg gcg atc att gct gat gtc ctt gac cca ggc atg atc 883Gly Glu Thr Leu Ala Ile Ile Ala Asp Val Leu Asp Pro Gly Met Ile
250 255 260atc att ggt ggc gga ctg tcc aat gct gcc gac ctt tat ttg gat cgc 931Ile Ile Gly Gly Gly Leu Ser Asn Ala Ala Asp Leu Tyr Leu Asp Arg
265 270 275tcg gtc aac cac tat tcc acc cgc atc gtc ggc gca gga tat cgc cct 979Ser Val Asn His Tyr Ser Thr Arg Ile Val Gly Ala Gly Tyr Arg Pro
280 285 290ttg gca cgc gtt gcc aca gct cag ttg ggt gcg gat gct ggc atg atc 1027Leu Ala Arg Val Ala Thr Ala Gln Leu Gly Ala Asp Ala Gly Met Ile
295 300 305ggt gtc gct gat cta gct cga cgc tct gta gtg gaa gcc aac 1069Gly Val Ala Asp Leu Ala Arg Arg Ser Val Val Glu Ala Asn310 315 320taggtgtttt tcggtgggct gcg 1092<210>24<211>323<212>PRT<213>谷氨酸棒杆菌<400>24Met Pro Gln Lys Pro Ala Ser Phe Ala Val Gly Phe Asp Ile Gly Gly1 5 10 15Thr Asn Met Arg Ala Gly Leu Val Asp Glu Ser Gly Arg Ile Val Thr
20 25 30Ser Leu Ser Ala Pro Ser Pro Arg Thr Thr Gln Ala Met Glu Gln Gly
35 40 45Ile Phe Asp Leu Val Glu Gln Leu Lys Ala Glu Tyr Pro Val Gly Ala
50 55 60Val Gly Leu Ala Val Ala Gly Phe Leu Asp Pro Glu Cys Glu Val Val65 70 75 80Arg Phe Ala Pro His Leu Pro Trp Arg Asp Glu Pro Val Arg Glu Lys
85 90 95Leu Glu Asn Leu Leu Gly Leu Pro Val Arg Leu Glu His Asp Ala Asn
100 105 110Ser Ala Ala Trp Gly Glu His Arg Phe Gly Ala Ala Gln Gly Ala Asp
115 120 125Asn Trp Val Leu Leu Ala Leu Gly Thr Gly Ile Gly Ala Ala Leu Ile
130 135 140Glu Lys Gly Glu Ile Tyr Arg Gly Ala Tyr Gly Thr Ala Pro Glu Phe145 150 155 160Gly His Leu Arg Val Val Arg Gly Gly Arg Ala Cys Ala Cys Gly Lys
165 170 175Glu Gly Cys Leu Glu Arg Tyr Cys Ser Gly Thr Ala Leu Val Tyr Thr
180 185 190Ala Arg Glu Leu Ala Ser His Gly Ser Phe Arg Asn Ser Gly Leu Phe
195 200 205Asp Lys Ile Lys Ala Asp Pro Asn Ser Ile Asn Gly Lys Thr Ile Thr
210 215 220Ala Ala Ala Arg Gln Glu Asp Pro Leu Ala Leu Ala Val Leu Glu Asp225 230 235 240Phe Ser Glu Trp Leu Gly Glu Thr Leu Ala Ile Ile Ala Asp Val Leu
245 250 255Asp Pro Gly Met Ile Ile Ile Gly Gly Gly Leu Ser Asn Ala Ala Asp
260 265 270Leu Tyr Leu Asp Arg Ser Val Asn His Tyr Ser Thr Arg Ile Val Gly
275 280 285Ala Gly Tyr Arg Pro Leu Ala Arg Val Ala Thr Ala Gln Leu Gly Ala
290 295 300Asp Ala Gly Met Ile Gly Val Ala Asp Leu Ala Arg Arg Ser Val Val305 310 315 320Glu Ala Asn<210>25<211>1785<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1762)<223>RXA01814<400>25tgttaagcca ccctactccg tgaattttgc cgtatctcgt gcgcacaatt gcttttgagg 60ggaagatgaa gagaaagtat tggtgtttta aggagcaaac atg gca cat gaa cgc 115
Met Ala His Glu Arg
1 5gcc ggg caa ctc gcc caa cca gaa gat ctc atc gat gtt gcg gaa ctg 163Ala Gly Gln Leu Ala Gln Pro Glu Asp Leu Ile Asp Val Ala Glu Leu
10 15 20gtc acc gca tat ttc acc cgc aag ccg gac gtg aac aac cct gat cag 211Val Thr Ala Tyr Phe Thr Arg Lys Pro Asp Val Asn Asn Pro Asp Gln
25 30 35cag gtc gct ttc ggc acc tcc gga cac cgt ggc ttc gcg ctg gac agc 259Gln Val Ala Phe Gly Thr Ser Gly His Arg Gly Phe Ala Leu Asp Ser
40 45 50gct ttc aac gag gac cac atc ctg gca acc acc cag gcg atc gtc gac 307Ala Phe Asn Glu Asp His Ile Leu Ala Thr Thr Gln Ala Ile Val Asp
55 60 65tac cgc aac cag cag cca aaa aac tgg gtc aac ccg ctg ttt atc ggc 355Tyr Arg Asn Gln Gln Pro Lys Asn Trp Val Gly Pro Leu Phe Ile Gly70 75 80 85cgc gat acg cac gcg ctg tcc gaa cca gcg atg atc agc gcg ctt gag 403Arg Asp Thr His Ala Leu Ser Glu Pro Ala Met Ile Ser Ala Leu Glu
90 95 100gtc ctc att gcc aac gac gtc gaa gtg ctt gtc gac gcc gac ggc cgc 451Val Leu Ile Ala Asn Asp Val Glu Val Leu Val Asp Ala Asp Gly Arg
105 110 115tac acc ccg acg ccc gca gtg tcc cac gcg atc cta cga cac aac gat 499Tyr Thr Pro Thr Pro Ala Val Ser His Ala Ile Leu Arg His Asn Asp
120 125 130ggc atc atc ctt ggc acc gca gga ccc tcc cgc ccc tac gcc gac ggc 547Gly Ile Ile Leu Gly Thr Ala Gly Pro Ser Arg Pro Tyr Ala Asp Gly
135 140 145atc gtg atc acc cca tcc cac aac cct cct cgt gat ggc gga ttc aaa 595Ile Val Ile Thr Pro Ser His Asn Pro Pro Arg Asp Gly Gly Phe Lys150 155 160 165tac aac cca gcc aac ggt ggc cct gca gat acc gac gcc acc gac tgg 643Tyr Asn Pro Ala Asn Gly Gly Pro Ala Asp Thr Asp Ala Thr Asp Trp
170 175 180atc gcc aac cgc gcc aac gat att ctg cgc ggc gac ctt gca gac gtg 691Ile Ala Asn Arg Ala Asn Asp Ile Leu Arg Gly Asp Leu Ala Asp Val
185 190 195aag cga gtt cca gtt tcc ggt gtc ctc gac gag cgc acc act gcc tac 739Lys Arg Val Pro Val Ser Gly Val Leu Asp Glu Arg Thr Thr Ala Tyr
200 205 210gac ttc aag ggc att tac atc gct gac ctg cca aac gtg gtc aac atc 787Asp Phe Lys Gly Ile Tyr Ile Ala Asp Leu Pro Asn Val Val Asn Ile
215 220 225gat gcc atc cgc gaa gct ggt gtt cga atc ggc gca gac cca atg ggt 835Asp Ala Ile Arg Glu Ala Gly Val Arg Ile Gly Ala Asp Pro Met Gly230 235 240 245ggc gca tcc gtg gat tac tgg ggt gcc atc gca gaa acc cat ggc ctc 883Gly Ala Ser Val Asp Tyr Trp Gly Ala Ile Ala Glu Thr His Gly Leu
250 255 260aac ctc acc gtg gtc aac cca cac gtt gat tcc acc ttc cgc ttc atg 931Asn Leu Thr Val Val Asn Pro His Val Asp Ser Thr Phe Arg Phe Met
265 270 275aca ttg gac acc gac ggc aag atc cgc atg gac tgc tcc agc cca cac 979Thr Leu Asp Thr Asp Gly Lys Ile Arg Met Asp Cys Ser Ser Pro His
280 285 290gca atg gca tcg ctg att gac aac cga gac aag ttc gat gtg gca acc 1027Ala Met Ala Ser Leu Ile Asp Asn Arg Asp Lys Phe Asp Val Ala Thr
295 300 305ggc aac gac gcc gac gcc gac cgc cac ggc atc gtc acc cca gac gct 1075Gly Asn Asp Ala Asp Ala Asp Arg His Gly Ile Val Thr Pro Asp Ala310 315 320 325ggc ttg atg aac ccc aac cac tac ctc gca gta gca att gag tac ctc 1123Gly Leu Met Asn Pro Asn His Tyr Leu Ala Val Ala Ile Glu Tyr Leu
330 335 340ttt gct cac cgc cca ggt tgg tcc gca gat acc gca gtg ggc aaa acc 1171Phe Ala His Arg Pro Gly Trp Ser Ala Asp Thr Ala Val Gly Lys Thr
345 350 355ctg gtc agc tcc tcc atg atc gac cgc gtt gtg gcg cag ctt ggc cgc 1219Leu Val Ser Ser Ser Met Ile Asp Arg Val Val Ala Gln Leu Gly Arg
360 365 370acc ctc gtt gag gtt cca gtc gga ttc aag tgg ttt gtc cca ggt ttg 1267Thr Leu Val Glu Val Pro Val Gly Phe Lys Trp Phe Val Pro Gly Leu
375 380 385atc tcc ggc gaa atc gga ttc ggt ggt gaa gaa tcc gca ggt gca tcc 1315Ile Ser Gly Glu Ile Gly Phe Gly Gly Glu Glu Ser Ala Gly Ala Ser390 395 400 405ttc ctc cgc atg gac ggc acc acc tgg tcc acc gac aag gac ggc ctc 1363Phe Leu Arg Met Asp Gly Thr Thr Trp Ser Thr Asp Lys Asp Gly Leu
410 415 420atc ctt gac ctc ctg gca gct gag atc att gca gta acc ggc aag acc 1411Ile Leu Asp Leu Leu Ala Ala Glu Ile Ile Ala Val Thr Gly Lys Thr
425 430 435cca tca cag cgc tac gca gaa ctc gcc gaa gaa ttc ggt gca cct gcc 1459Pro Ser Gln Arg Tyr Ala Glu Leu Ala Glu Glu Phe Gly Ala Pro Ala
440 445 450tac gcc cgc acc gat gca gaa gcc aac cga gaa caa aag gcc atc ctg 1507Tyr Ala Arg Thr Asp Ala Glu Ala Asn Arg Glu Gln Lys Ala Ile Leu
455 460 465aag gca ctg tcc cca gaa cag gtc acc gcc acc gaa cta gcc ggc gaa 1555Lys Ala Leu Ser Pro Glu Gln Val Thr Ala Thr Glu Leu Ala Gly Glu470 475 480 485gca atc acc gct aag ctc acc gaa gct ccc ggc aat ggc gca gcc atc 1603Ala Ile Thr Ala Lys Leu Thr Glu Ala Pro Gly Asn Gly Ala Ala Ile
490 495 500gga gga cta aaa gtg acc acc gaa aac gcc tgg ttc gca gca cgc cca 1651Gly Gly Leu Lys Val Thr Thr Glu Asn Ala Trp Phe Ala Ala Arg Pro
505 510 515tcc ggc acc gaa gac aag tac aag atc tac gca gaa tcc ttc aag ggc 1699Ser Gly Thr Glu Asp Lys Tyr Lys Ile Tyr Ala Glu Ser Phe Lys Gly
520 525 530gaa gag cac ctc gcc cag gtt cag aag gaa gcc caa gcg ttg gtc agc 1747Glu Glu His Leu Ala Gln Val Gln Lys Glu Ala Gln Ala Leu Val Ser
535 540 545gaa gta ctc gga cag taaaactgcg gacttgctga caa 1785Glu Val Leu Gly Gln550<210>26<211>554<212>PRT<213>谷氨酸棒杆菌<400>26Met Ala His Glu Arg Ala Gly Gln Leu Ala Gln Pro Glu Asp Leu Ile1 5 10 15Asp Val Ala Glu Leu Val Thr Ala Tyr Phe Thr Arg Lys Pro Asp Val
20 25 30Asn Asn Pro Asp Gln Gln Val Ala Phe Gly Thr Ser Gly His Arg Gly
35 40 45Phe Ala Leu Asp Ser Ala Phe Asn Glu Asp His Ile Leu Ala Thr Thr
50 55 60Gln Ala Ile Val Asp Tyr Arg Asn Gln Gln Pro Lys Asn Trp Val Gly65 70 75 80Pro Leu Phe Ile Gly Arg Asp Thr His Ala Leu Ser Glu Pro Ala Met
85 90 95Ile Ser Ala Leu Glu Val Leu Ile Ala Asn Asp Val Glu Val Leu Val
100 105 110Asp Ala Asp Gly Arg Tyr Thr Pro Thr Pro Ala Val Ser His Ala Ile
115 120 125Leu Arg His Asn Asp Gly Ile Ile Leu Gly Thr Ala Gly Pro Ser Arg
130 135 140Pro Tyr Ala Asp Gly Ile Val Ile Thr Pro Ser His Asn Pro Pro Arg145 150 155 160Asp Gly Gly Phe Lys Tyr Asn Pro Ala Asn Gly Gly Pro Ala Asp Thr
165 170 175Asp Ala Thr Asp Trp Ile Ala Asn Arg Ala Asn Asp Ile Leu Arg Gly
180 185 190Asp Leu Ala Asp Val Lys Arg Val Pro Val Ser Gly Val Leu Asp Glu
195 200 205Arg Thr Thr Ala Tyr Asp Phe Lys Gly Ile Tyr Ile Ala Asp Leu Pro
210 215 220Asn Val Val Asn Ile Asp Ala Ile Arg Glu Ala Gly Val Arg Ile Gly225 230 235 240Ala Asp Pro Met Gly Gly Ala Ser Val Asp Tyr Trp Gly Ala Ile Ala
245 250 255Glu Thr His Gly Leu Asn Leu Thr Val Val Asn Pro His Val Asp Ser
260 265 270Thr Phe Arg Phe Met Thr Leu Asp Thr Asp Gly Lys Ile Arg Met Asp
275 280 285Cys Ser Ser Pro His Ala Met Ala Ser Leu Ile Asp Asn Arg Asp Lys
290 295 300Phe Asp Val Ala Thr Gly Asn Asp Ala Asp Ala Asp Arg His Gly Ile305 310 315 320Val Thr Pro Asp Ala Gly Leu Met Asn Pro Asn His Tyr Leu Ala Val
325 330 335Ala Ile Glu Tyr Leu Phe Ala His Arg Pro Gly Trp Ser Ala Asp Thr
340 345 350Ala Val Gly Lys Thr Leu Val Ser Ser Ser Met Ile Asp Arg Val Val
355 360 365Ala Gln Leu Gly Arg Thr Leu Val Glu Val Pro Val Gly Phe Lys Trp
370 375 380Phe Val Pro Gly Leu Ile Ser Gly Glu Ile Gly Phe Gly Gly Glu Glu385 390 395 400Ser Ala Gly Ala Ser Phe Leu Arg Met Asp Gly Thr Thr Trp Ser Thr
405 410 415Asp Lys Asp Gly Leu Ile Leu Asp Leu Leu Ala Ala Glu Ile Ile Ala
420 425 430Val Thr Gly Lys Thr Pro Ser Gln Arg Tyr Ala Glu Leu Ala Glu Glu
435 440 445Phe Gly Ala Pro Ala Tyr Ala Arg Thr Asp Ala Glu Ala Asn Arg Glu
450 455 460Gln Lys Ala Ile Leu Lys Ala Leu Ser Pro Glu Gln Val Thr Ala Thr465 470 475 480Glu Leu Ala Gly Glu Ala Ile Thr Ala Lys Leu Thr Glu Ala Pro Gly
485 490 495Asn Gly Ala Ala Ile Gly Gly Leu Lys Val Thr Thr Glu Asn Ala Trp
500 505 510Phe Ala Ala Arg Pro Ser Gly Thr Glu Asp Lys Tyr Lys Ile Tyr Ala
515 520 525Glu Ser Phe Lys Gly Glu Glu His Leu Ala Gln Val Gln Lys Glu Ala
530 535 540Gln Ala Leu Val Ser Glu Val Leu Gly Gln545 550<210>27<211>680<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(657)<223>RXN02803<400>27gtc tct gga gag atg ctc gcg gca gca ctt tca gca ggc atg gcc agc 48Val Ser Gly Glu Met Leu Ala Ala Ala Leu Ser Ala Gly Met Ala Ser1 5 10 15cag ggt gtt gat gtc att cgt gtt ggt gtc atc cca acc cca gct gtt 96Gln Gly Val Asp Val Ile Arg Val Gly Val Ile Pro Thr Pro Ala Val
20 25 30gca ttc ctc acc gat gat tat ggc gct gac atg ggc gtg atg att tct 144Ala Phe Leu Thr Asp Asp Tyr Gly Ala Asp Met Gly Val Met Ile Ser
35 40 45gca tcc cac aac cca atg ccg gac aac gga atc aag ttc ttt tct gca 192Ala Ser His Asn Pro Met Pro Asp Asn Gly Ile Lys Phe Phe Ser Ala
50 55 60ggt gga cac aag ctt cca gac cat gtg gaa gac gag att gag cgt gtt 240Gly Gly His Lys Leu Pro Asp His Val Glu Asp Glu Ile Glu Arg Val65 70 75 80atg gac agc ttg cca gca gaa ggc cca aca ggg cat gga gtt ggc cgt 288Met Asp Ser Leu Pro Ala Glu Gly Pro Thr Gly His Gly Val Gly Arg
85 90 95gtc atc gaa gaa gca acc gat gca cag gac cgt tac cta gag cac ctg 336Val Ile Glu Glu Ala Thr Asp Ala Gln Asp Arg Tyr Leu Glu His Leu
100 105 110aag gaa gct gtt cct acg tca ctt gaa ggc atc aag att gtt gtg gat 384Lys Glu Ala Val Pro Thr Ser Leu Glu Gly Ile Lys Ile Val Val Asp
115 120 125gca gcc aat ggt gcg gca agt gtt gta gct cca acg gct tat gag gct 432Ala Ala Asn Gly Ala Ala Ser Val Val Ala Pro Thr Ala Tyr Glu Ala
130 135 140gcg ggt gca act gta att gct att cat aac aag cca gac tca tac aac 480Ala Gly Ala Thr Val Ile Ala Ile His Asn Lys Pro Asp Ser Tyr Asn145 150 155 160atc aac atg gac tgc ggt tcc acc cac att gat cag gcg cag ccg cca 528Ile Asn Met Asp Cys Gly Ser Thr His Ile Asp Gln Ala Gln Pro Pro
165 170 175gtc ttg aag cac ggt gct gac ctt gga ctc gcg cat gac ggt gat gct 576Val Leu Lys His Gly Ala Asp Leu Gly Leu Ala His Asp Gly Asp Ala
180 185 190gac cgt tgt ttg gct gtg aac aag gat gcc aac ctt gtt gat ggt gac 624Asp Arg Cys Leu Ala Val Asn Lys Asp Ala Asn Leu Val Asp Gly Asp
195 200 205caa atc atg gcg ctg tta gcc att gcg atg aaa taaaacggcg agctgcgcaa 677Gln Ile Met Ala Leu Leu Ala Ile Ala Met Lys
210 215gaa 680<210>28<211>219<212>PRT<213>谷氨酸棒杆菌<400> 28Val Ser Gly Glu Met Leu Ala Ala Ala Leu Ser Ala Gly Met Ala Ser1 5 10 15Gln Gly Val Asp Val Ile Arg Val Gly Val Ile Pro Thr Pro Ala Val
20 25 30Ala Phe Leu Thr Asp Asp Tyr Gly Ala Asp Met Gly Val Met Ile Ser
35 40 45Ala Ser His Asn Pro Met Pro Asp Asn Gly Ile Lys Phe Phe Ser Ala
50 55 60Gly Gly His Lys Leu Pro Asp His Val Glu Asp Glu Ile Glu Arg Val65 70 75 80Met Asp Ser Leu Pro Ala Glu Gly Pro Thr Gly His Gly Val Gly Arg
85 90 95Val Ile Glu Glu Ala Thr Asp Ala Gln Asp Arg Tyr Leu Glu His Leu
100 105 110Lys Glu Ala Val Pro Thr Ser Leu Glu Gly Ile Lys Ile Val Val Asp
115 120 125Ala Ala Asn Gly Ala Ala Ser Val Val Ala Pro Thr Ala Tyr Glu Ala
130 135 140Ala Gly Ala Thr Val Ile Ala Ile His Asn Lys Pro Asp Ser Tyr Asn145 150 155 160Ile Asn Met Asp Cys Gly Ser Thr His Ile Asp Gln Ala Gln Pro Pro
165 170 175Val Leu Lys His Gly Ala Asp Leu Gly Leu Ala His Asp Gly Asp Ala
180 185 190Asp Arg Cys Leu Ala Val Asn Lys Asp Ala Asn Leu Val Asp Gly Asp
195 200 205Gln Ile Met Ala Leu Leu Ala Ile Ala Met Lys
210 215<210>29<211>399<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(399)<223>FRXA02803<400>29tct gga gag atg ctc gcg gca gca ctt tca gca ggc atg gcc agc cag 48Ser Gly Glu Met Leu Ala Ala Ala Leu Ser Ala Gly Met Ala Ser Gln1 5 10 15ggt gtt gat gtc att cgt gtt ggt gtc atc cca acc cca gct gtt gca 96Gly Val Asp Val Ile Arg Val Gly Val Ile Pro Thr Pro Ala Val Ala
20 25 30ttc ctc acc gat gat tat ggc gct gac atg ggc gtg atg att tct gca 144Phe Leu Thr Asp Asp Tyr Gly Ala Asp Met Gly Val Met Ile Ser Ala
35 40 45tcc cac aac cca atg ccg gac aac gga atc aag ttc ttt tct gca ggt 192Ser His Asn Pro Met Pro Asp Asn Gly Ile Lys Phe Phe Ser Ala Gly
50 55 60gga cac aag ctt cca gac cat gtg gaa gac gag att gag cgt gtt atg 240Gly His Lys Leu Pro Asp His Val Glu Asp Glu Ile Glu Arg Val Met65 70 75 80gac agc ttg cca gca gaa ggc cca aca ggg cat gga gtt ggc cgt gtc 288Asp Ser Leu Pro Ala Glu Gly Pro Thr Gly His Gly Val Gly Arg Val
85 90 95atc gaa gaa gca acc gat gca cag gac cgc tac cta gag cac ctg aag 336Ile Glu Glu Ala Thr Asp Ala Gln Asp Arg Tyr Leu Glu His Leu Lys
100 105 110gaa gct gtt cct acg tca ctt gaa ggc atc aag att gtt gtg gat gca 384Glu Ala Val Pro Thr Ser Leu Glu Gly Ile Lys Ile Val Val Asp Ala
115 120 125gcc aat ggt gcg gca 399Ala Asn Gly Ala Ala
130<210>30<211>133<212>PRT<213>谷氨酸棒杆菌<400>30Ser Gly Glu Met Leu Ala Ala Ala Leu Ser Ala Gly Met Ala Ser Gln1 5 10 15Gly Val Asp Val Ile Arg Val Gly Val Ile Pro Thr Pro Ala Val Ala
20 25 30Phe Leu Thr Asp Asp Tyr Gly Ala Asp Met Gly Val Met Ile Ser Ala
35 40 45Ser His Asn Pro Met Pro Asp Asn Gly Ile Lys Phe Phe Ser Ala Gly
50 55 60Gly His Lys Leu Pro Asp His Val Glu Asp Glu Ile Glu Arg Val Met65 70 75 80Asp Ser Leu Pro Ala Glu Gly Pro Thr Gly His Gly Val Gly Arg Val
85 90 95Ile Glu Glu Ala Thr Asp Ala Gln Asp Arg Tyr Leu Glu His Leu Lys
100 105 110Glu Ala Val Pro Thr Ser Leu Glu Gly Ile Lys Ile Val Val Asp Ala
115 120 125Ala Asn Gly Ala Ala
130<210>31<21l>1713<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1690)<223>RXN03076<400>31aatacctttc tgttttgtcc gcaggcgtat caggaaaacc tgcagcgcgg tagagtcgag 60tctaatagtg atcccacgaa aacaaaggat cggggtgttc atg gac gag tct cgt 115
Met Asp Glu Ser Arg
1 5cag ctt agt ttc ggc aca gca ggg ttg cgt gca cca gtt ggc ccg gcg 163Gln Leu Ser Phe Gly Thr Ala Gly Leu Arg Ala Pro Val Gly Pro Ala
10 15 20cgc cat cag atg aat gtt ttg cag gta acc aga act aca gca ggt gtt 211Arg His Gln Met Asn Val Leu Gln Val Thr Arg Thr Thr Ala Gly Val
25 30 35gct agt tgg ttg gca gaa cgt gcg gca cta aat cca gtg ccg cat ttg 259Ala Ser Trp Leu Ala Glu Arg Ala Ala Leu Asn Pro Val Pro His Leu
40 45 50gtt cct gag gat gaa aca gga atc ggc agg gcg ttg tat ccc caa gat 307Val Pro Glu Asp Glu Thr Gly Ile Gly Arg Ala Leu Tyr Pro Gln Asp
55 60 65ggt ccg ttg cgg gtc gtt gtg ggg tat gac gct cgc tat ggt tcg cat 355Gly Pro Leu Arg Val Val Val Gly Tyr Asp Ala Arg Tyr Gly Ser His70 75 80 85act ttt gct gca acc act gcg gag gtg ttc gcg ggt gct ggt ttt gag 403Thr Phe Ala Ala Thr Thr Ala Glu Val Phe Ala Gly Ala Gly Phe Glu
90 95 100gtg acg ttg ctc ccc acg cct agc cct acg ccg ttg att ccg tgg ttg 451Val Thr Leu Leu Pro Thr Pro Ser Pro Thr Pro Leu Ile Pro Trp Leu
105 110 115gtg aac aag cat ggg ttg gat gcg ggc gtt cag atc acg gct tcg cat 499Val Asn Lys His Gly Leu Asp Ala Gly Val Gln Ile Thr Ala Ser His
120 125 130aat ggt gcg gcg gac aat ggc tac aag gtg ttt ttg tct aat ggt cgc 547Asn Gly Ala Ala Asp Asn Gly Tyr Lys Val Phe Leu Ser Asn Gly Arg
135 140 145cag ctt tat tct gaa ctg gag cct gag ctt gag gcg cat atc aat gct 595Gln Leu Tyr Ser Glu Leu Glu Pro Glu Leu Glu Ala His Ile Asn Ala150 155 160 165gtg gaa gat ccg att cgg gtt cct cgg gtg acg gtg cgc ccc act gct 643Val Glu Asp Pro Ile Arg Val Pro Arg Val Thr Val Arg Pro Thr Ala
170 175 180gat cag ctg cgt cga tat gtt gat gag atg gtg tcg ttg gtg act cct 691Asp Gln Leu Arg Arg Tyr Val Asp Glu Met Val Ser Leu Val Thr Pro
185 190 195gat cag gct gat ttg ttg cgg gtg aat tct gag cgg ggc aat ctt cgc 739Asp Gln Ala Asp Leu Leu Arg Val Asn Ser Glu Arg Gly Asn Leu Arg
200 205 210gtg gtg tat acc gct ctg cat ggt gtg ggt ggc cgc gcg atg gcc aat 787Val Val Tyr Thr Ala Leu His Gly Val Gly Gly Arg Ala Met Ala Asn
215 220 225gct ttc caa ttt gct ggt ttt ccc cat act cat ggc gtg aag gct cag 835Ala Phe Gln Phe Ala Gly Phe Pro His Thr His Gly Val Lys Ala Gln230 235 240 245cag tat cct gat ccc acc ttc ccc act gtg gcg ttc ccc aat ccg gaa 883Gln Tyr Pro Asp Pro Thr Phe Pro Thr Val Ala Phe Pro Asn Pro Glu
250 255 260gag cct tct gcg att gag ttg ttg ttg gaa cgc gca aag gaa aag aac 931Glu Pro Ser Ala Ile Glu Leu Leu Leu Glu Arg Ala Lys Glu Lys Asn
265 270 275gct gac att ttg ttt gcg ctt gat cct gat gcc gat cgt tgt gct gtg 979Ala Asp Ile Leu Phe Ala Leu Asp Pro Asp Ala Asp Arg Cys Ala Val
280 285 290ggt att cgt acc gct gat ggc ggc cac cga atg ctc tct ggc gat gag 1027Gly Ile Arg Thr Ala Asp Gly Gly His Arg Met Leu Ser Gly Asp Glu
295 300 305gtg ggc aca ctt ttg gct act cgt ttg gtt ccg gag tat tcc ggt gaa 1075Val Gly Thr Leu Leu Ala Thr Arg Leu Val Pro Glu Tyr Ser Gly Glu310 315 320 325ggc cca cgt ccc gtg gtt gcc acc acg gtg gtg tct tcg cag ctt ctg 1123Gly Pro Arg Pro Val Val Ala Thr Thr Val Val Ser Ser Gln Leu Leu
330 335 340ggt atc atc gcc gag gat aaa ggg tgg gat tat tcc gag aca ctg acg 1171Gly Ile Ile Ala Glu Asp Lys Gly Trp Asp Tyr Ser Glu Thr Leu Thr
345 350 355gga ttc aaa aat ctg tcg agg gct gcc gat ggt ctc gac gga ccg ctt 1219Gly Phe Lys Asn Leu Ser Arg Ala Ala Asp Gly Leu Asp Gly Pro Leu
360 365 370gct ttc gct tat gag gaa gct gtg ggc acc tgc ccg gtt cca gat gtc 1267Ala Phe Ala Tyr Glu Glu Ala Val Gly Thr Cys Pro Val Pro Asp Val
375 380 385gtg ccg gat aag gac ggc atc tct aca gcg ttg ttc atg gcg tcg tgg 1315Val Pro Asp Lys Asp Gly Ile Ser Thr Ala Leu Phe Met Ala Ser Trp390 395 400 405gct gcc gaa ctg aag gct cag ggc gca agc ctg cag caa aaa ctc aat 1363Ala Ala Glu Leu Lys Ala Gln Gly Ala Ser Leu Gln Gln Lys Leu Asn
410 415 420gag ttg tat cgc cga tat ggg tat ttt gcg tcc tcg caa att gct gtg 1411Glu Leu Tyr Arg Arg Tyr Gly Tyr Phe Ala Ser Ser Gln Ile Ala Val
425 430 435cgc acg agc agt cca cgc gag tta gtt gat cac tgg att gcg cat cct 1459Arg Thr Ser Ser Pro Arg Glu Leu Val Asp His Trp Ile Ala His Pro
440 445 450cag caa gaa ctc att gga gtg tct gtc acc cca cat att ctt cct gaa 1507Gln Gln Glu Leu Ile Gly Val Ser Val Thr Pro His Ile Leu Pro Glu
455 460 465aaa cag ggc att gct ttg cat ggc cag gtg ggg cat gtg cat atc cgt 1555Lys Gln Gly Ile Ala Leu His Gly Gln Val Gly His Val His Ile Arg470 475 480 485gct att ggt cga gtc tct gga act gag gcg aaa gcc aag ctc tat ttg 1603Ala Ile Gly Arg Val Ser Gly Thr Glu Ala Lys Ala Lys Leu Tyr Leu
490 495 500gaa gtt ggt cag gcc agc tcc cat gat gaa gca gct cag ttg ttg cat 1651Glu Val Gly Gln Ala Ser Ser His Asp Glu Ala Ala Gln Leu Leu His
505 510 515cag ctg gag gat gaa gtc caa agc tgg ttg agc aag ctt tagtttcctg 1700Gln Leu Glu Asp Glu Val Gln Ser Trp Leu Ser Lys Leu
520 525 530gctgctcccg gtt 1713<210>32<211>530<212>PRT<213>谷氨酸棒杆菌<400> 32Met Asp Glu Ser Arg Gln Leu Ser Phe Gly Thr Ala Gly Leu Arg Ala1 5 10 15Pro Val Gly Pro Ala Arg His Gln Met Asn Val Leu Gln Val Thr Arg
20 25 30Thr Thr Ala Gly Val Ala Ser Trp Leu Ala Glu Arg Ala Ala Leu Asn
35 40 45Pro Val Pro His Leu Val Pro Glu Asp Glu Thr Gly Ile Gly Arg Ala
50 55 60Leu Tyr Pro Gln Asp Gly Pro Leu Arg Val Val Val Gly Tyr Asp Ala65 70 75 80Arg Tyr Gly Ser His Thr Phe Ala Ala Thr Thr Ala Glu Val Phe Ala
85 90 95Gly Ala Gly Phe Glu Val Thr Leu Leu Pro Thr Pro Ser Pro Thr Pro
100 105 110Leu Ile Pro Trp Leu Val Asn Lys His Gly Leu Asp Ala Gly Val Gln
115 120 125Ile Thr Ala Ser His Asn Gly Ala Ala Asp Asn Gly Tyr Lys Val Phe
130 135 140Leu Ser Asn Gly Arg Gln Leu Tyr Ser Glu Leu Glu Pro Glu Leu Glu145 150 155 160Ala His Ile Asn Ala Val Glu Asp Pro Ile Arg Val Pro Arg Val Thr
165 170 175Val Arg Pro Thr Ala Asp Gln Leu Arg Arg Tyr Val Asp Glu Met Val
180 185 190Ser Leu Val Thr Pro Asp Gln Ala Asp Leu Leu Arg Val Asn Ser Glu
195 200 205Arg Gly Asn Leu Arg Val Val Tyr Thr Ala Leu His Gly Val Gly Gly
210 215 220Arg Ala Met Ala Asn Ala Phe Gln Phe Ala Gly Phe Pro His Thr His225 230 235 240Gly Val Lys Ala Gln Gln Tyr Pro Asp Pro Thr Phe Pro Thr Val Ala
245 250 255Phe Pro Asn Pro Glu Glu Pro Ser Ala Ile Glu Leu Leu Leu Glu Arg
260 265 270Ala Lys Glu Lys Asn Ala Asp Ile Leu Phe Ala Leu Asp Pro Asp Ala
275 280 285Asp Arg Cys Ala Val Gly Ile Arg Thr Ala Asp Gly Gly His Arg Met
290 295 300Leu Ser Gly Asp Glu Val Gly Thr Leu Leu Ala Thr Arg Leu Val Pro305 310 315 320Glu Tyr Ser Gly Glu Gly Pro Arg Pro Val Val Ala Thr Thr Val Val
325 330 335Ser Ser Gln Leu Leu Gly Ile Ile Ala Glu Asp Lys Gly Trp Asp Tyr
340 345 350Ser Glu Thr Leu Thr Gly Phe Lys Asn Leu Ser Arg Ala Ala Asp Gly
355 360 365Leu Asp Gly Pro Leu Ala Phe Ala Tyr Glu Glu Ala Val Gly Thr Cys
370 375 380Pro Val Pro Asp Val Val Pro Asp Lys Asp Gly Ile Ser Thr Ala Leu385 390 395 400Phe Met Ala Ser Trp Ala Ala Glu Leu Lys Ala Gln Gly Ala Ser Leu
405 410 415Gln Gln Lys Leu Asn Glu Leu Tyr Arg Arg Tyr Gly Tyr Phe Ala Ser
420 425 430Ser Gln Ile Ala Val Arg Thr Ser Ser Pro Arg Glu Leu Val Asp His
435 440 445Trp Ile Ala His Pro Gln Gln Glu Leu Ile Gly Val Ser Val Thr Pro
450 455 460His Ile Leu Pro Glu Lys Gln Gly Ile Ala Leu His Gly Gln Val Gly465 470 475 480His Val His Ile Arg Ala Ile Gly Arg Val Ser Gly Thr Glu Ala Lys
485 490 495Ala Lys Leu Tyr Leu Glu Val Gly Gln Ala Ser Ser His Asp Glu Ala
500 505 510Ala Gln Leu Leu His Gln Leu Glu Asp Glu Val Gln Ser Trp Leu Ser
515 520 525Lys Leu
530<210>33<211>1684<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1684)<223>FRXA02854<400>33aatacctttc tgttttgtcc gcaggcgtat caggaaaacc tgcagcgcgg tagagtcgag 60tctaatagtg atcccacgaa aacaaaggat cggggtgttc atg gac gag tct cgt 115
Met Asp Glu Ser Arg
1 5cag ctt agt ttc ggc aca gca ggg ttg cgt gca cca gtt ggc ccg gcg 163Gln Leu Ser Phe Gly Thr Ala Gly Leu Arg Ala Pro Val Gly Pro Ala
10 15 20cgc cat cag atg aat gtt ttg cag gta acc aga act aca gca ggt gtt 211Arg His Gln Met Asn Val Leu Gln Val Thr Arg Thr Thr Ala Gly Val
25 30 35gct agt tgg ttg gca gaa cgt gcg gca cta aat cca gtg ccg cat ttg 259Ala Ser Trp Leu Ala Glu Arg Ala Ala Leu Asn Pro Val Pro His Leu
40 45 50gtt cct gag gat gaa aca gga atc ggc agg gcg ttg tat ccc caa gat 307Val Pro Glu Asp Glu Thr Gly Ile Gly Arg Ala Leu Tyr Pro Gln Asp
55 60 65ggt ccg ttg cgg gtc gtt gtg ggg tat gac gct cgc tat ggt tcg cat 355Gly Pro Leu Arg Val Val Val Gly Tyr Asp Ala Arg Tyr Gly Ser His70 75 80 85act ttt gct gca acc act gcg gag gtg ttc gcg ggt gct ggt ttt gag 403Thr Phe Ala Ala Thr Thr Ala Glu Val Phe Ala Gly Ala Gly Phe Glu
90 95 100gtg acg ttg ctc ccc acg cct agc cct acg ccg ttg att ccg tgg ttg 451Val Thr Leu Leu Pro Thr Pro Ser Pro Thr Pro Leu Ile Pro Trp Leu
105 110 115gtg aac aag cat ggg ttg gat gcg ggc gtt cag atc acg gct tcg cat 499Val Asn Lys His Gly Leu Asp Ala Gly Val Gln Ile Thr Ala Ser His
120 125 130aat ggt gcg gcg gac aat ggc tac aag gtg ttt ttg tct aat ggt cgc 547Asn Gly Ala Ala Asp Asn Gly Tyr Lys Val Phe Leu Ser Asn Gly Arg
135 140 145cag ctt tat tct gaa ctg gag cct gag ctt gag gcg cat atc aat gct 595Gln Leu Tyr Ser Glu Leu Gln Pro Glu Leu Glu Ala His Ile Asn Ala150 155 160 165gtg gaa gat ccg att cgg gtt cct cgg gtg acg gtg cgc ccc act gct 643Val Glu Asp Pro Ile Arg Val Pro Arg Val Thr Val Arg Pro Thr Ala
170 175 180gat cag ctg cgt cga tat gtt gat gag atg gtg tcg ttg gtg act cct 691Asp Gln Leu Arg Arg Tyr Val Asp Glu Met Val Ser Leu Val Thr Pro
185 190 195gat cag gct gat ttg ttg cgg gtg aat tct gag cgg ggc aat ctt cgc 739Asp Gln Ala Asp Leu Leu Arg Val Asn Ser Glu Arg Gly Asn Leu Arg
200 205 210gtg gtg tat acc gct ctg cat ggt gtg ggt ggc cgc gcg atg gcc aat 787Val Val Tyr Thr Ala Leu His Gly Val Gly Gly Arg Ala Met Ala Asn
215 220 225gct ttc caa ttt gct ggt ttt ccc cat act cat ggc gtg aag gct cag 835Ala Phe Gln Phe Ala Gly Phe Pro His Thr His Gly Val Lys Ala Gln230 235 240 245cag tat cct gat ccc acc ttc ccc act gtg gcg ttc ccc aat ccg gaa 883Gln Tyr Pro Asp Pro Thr Phe Pro Thr Val Ala Phe Pro Asn Pro Glu
250 255 260gag cct tct gcg att gag ttg ttg ttg gaa cgc gca aag gaa aag aac 931Glu Pro Ser Ala Ile Glu Leu Leu Leu Glu Arg Ala Lys Glu Lys Asn
265 270 275gct gac att ttg ttt gcg ctt gat cct gat gcc gat cgt tgt gct gtg 979Ala Asp Ile Leu Phe Ala Leu Asp Pro Asp Ala Asp Arg Cys Ala Val
280 285 290ggt att cgt acc gct gat ggc ggc cac cga atg ctc tct ggc gat gag 1027Gly Ile Arg Thr Ala Asp Gly Gly His Arg Met Leu Ser Gly Asp Glu
295 300 305gtg ggc aca ctt ttg gct act cgt ttg gtt ccg gag tat tcc ggt gaa 1075Val Gly Thr Leu Leu Ala Thr Arg Leu Val Pro Glu Tyr Ser Gly Glu310 315 320 325ggc cca cgt ccc gtg gtt gcc acc acg gtg gtg tct tcg cag ctt ctg 1123Gly Pro Arg Pro Val Val Ala Thr Thr Val Val Ser Ser Gln Leu Leu
330 335 340ggt atc atc gcc gag gat aaa ggg tgg gat tat tcc gag aca ctg acg 1171Gly Ile Ile Ala Glu Asp Lys Gly Trp Asp Tyr Ser Glu Thr Leu Thr
345 350 355gga ttc aaa aat ctg tcg agg gct gcc gat ggt ctc gac gga ccg ctt 1219Gly Phe Lys Asn Leu Ser Arg Ala Ala Asp Gly Leu Asp Gly Pro Leu
360 365 370gct ttc gct tat gag gaa gct gtg ggc acc tgc ccg gtt cca gat gtc 1267Ala Phe Ala Tyr Glu Glu Ala Val Gly Thr Cys Pro Val Pro Asp Val
375 380 385gtg ccg gat aag gac ggc atc tct aca gcg ttg ttc atg gcg tcg tgg 1315Val Pro Asp Lys Asp Gly Ile Ser Thr Ala Leu Phe Met Ala Ser Trp390 395 400 405gct gcc gaa ctg aag gct cag ggc gca agc ctg cag caa aaa ctc aat 1363Ala Ala Glu Leu Lys Ala Gln Gly Ala Ser Leu Gln Gln Lys Leu Asn
410 415 420gag ttg tat cgc cga tat ggg tat ttt gcg tcc tcg caa att gct gtg 1411Glu Leu Tyr Arg Arg Tyr Gly Tyr Phe Ala Ser Ser Gln Ile Ala Val
425 430 435cgc acg agc agt cca cgc gag tta gtt gat cac tgg att gcg cat cct 1459Arg Thr Ser Ser Pro Arg Glu Leu Val Asp His Trp Ile Ala His Pro
440 445 450cag caa gaa ctc att gga gtg tct gtc acc cca cat att ctt cct gaa 1507Gln Gln Glu Leu Ile Gly Val Ser Val Thr Pro His Ile Leu Pro Glu
455 460 465aaa cag ggc att gct ttg cat ggc cag gtg ggg cat gtg cat atc cgt 1555Lys Gln Gly Ile Ala Leu His Gly Gln Val Gly His Val His Ile Arg470 475 480 485gct att ggt cga gtc tct gga act gag gcg aaa gcc aag ctc tat ttg 1603Ala Ile Gly Arg Val Ser Gly Thr Glu Ala Lys Ala Lys Leu Tyr Leu
490 495 500gaa gtt ggt cag gcc agc tcc cat gat gaa gca gct cag ttg ttg cat 1651Glu Val Gly Gln Ala Ser Ser His Asp Glu Ala Ala Gln Leu Leu His
505 510 515cag ctg gag gat gaa gtc caa agc tgg ttg agc 1684Gln Leu Glu Asp Glu Val Gln Ser Trp Leu Ser
520 525<210>34<211>528<212>PRT<213>谷氨酸棒杆菌<400>34Met Asp Glu Ser Arg Gln Leu Ser Phe Gly Thr Ala Gly Leu Arg Ala1 5 10 15Pro Val Gly Pro Ala Arg His Gln Met Asn Val Leu Gln Val Thr Arg
20 25 30Thr Thr Ala Gly Val Ala Ser Trp Leu Ala Glu Arg Ala Ala Leu Asn
35 40 45Pro Val Pro His Leu Val Pro Glu Asp Glu Thr Gly Ile Gly Arg Ala
50 55 60Leu Tyr Pro Gln Asp Gly Pro Leu Arg Val Val Val Gly Tyr Asp Ala65 70 75 80Arg Tyr Gly Ser His Thr Phe Ala Ala Thr Thr Ala Glu Val Phe Ala
85 90 95Gly Ala Gly Phe Glu Val Thr Leu Leu Pro Thr Pro Ser Pro Thr Pro
100 105 110Leu Ile Pro Trp Leu Val Asn Lys His Gly Leu Asp Ala Gly Val Gln
115 120 125Ile Thr Ala Ser His Asn Gly Ala Ala Asp Asn Gly Tyr Lys Val Phe
130 135 140Leu Ser Asn Gly Arg Gln Leu Tyr Ser Glu Leu Glu Pro Glu Leu Glu145 150 155 160Ala His Ile Asn Ala Val Glu Asp Pro Ile Arg Val Pro Arg Val Thr
165 170 175Val Arg Pro Thr Ala Asp Gln Leu Arg Arg Tyr Val Asp Glu Met Val
180 185 190Ser Leu Val Thr Pro Asp Gln Ala Asp Leu Leu Arg Val Asn Ser Glu
195 200 205Arg Gly Asn Leu Arg Val Val Tyr Thr Ala Leu His Gly Val Gly Gly
210 215 220Arg Ala Met Ala Asn Ala Phe Gln Phe Ala Gly Phe Pro His Thr His225 230 235 240Gly Val Lys Ala Gln Gln Tyr Pro Asp Pro Thr Phe Pro Thr Val Ala
245 250 255Phe Pro Asn Pro Glu Glu Pro Ser Ala Ile Glu Leu Leu Leu Glu Arg
260 265 270Ala Lys Glu Lys Asn Ala Asp Ile Leu Phe Ala Leu Asp Pro Asp Ala
275 280 285Asp Arg Cys Ala Val Gly Ile Arg Thr Ala Asp Gly Gly His Arg Met
290 295 300Leu Ser Gly Asp Glu Val Gly Thr Leu Leu Ala Thr Arg Leu Val Pro305 310 315 320Glu Tyr Ser Gly Glu Gly Pro Arg Pro Val Val Ala Thr Thr Val Val
325 330 335Ser Ser Gln Leu Leu Gly Ile Ile Ala Glu Asp Lys Gly Trp Asp Tyr
340 345 350Ser Glu Thr Leu Thr Gly Phe Lys Asn Leu Ser Arg Ala Ala Asp Gly
355 360 365Leu Asp Gly Pro Leu Ala Phe Ala Tyr Glu Glu Ala Val Gly Thr Cys
370 375 380Pro Val Pro Asp Val Val Pro Asp Lys Asp Gly Ile Ser Thr Ala Leu385 390 395 400Phe Met Ala Ser Trp Ala Ala Glu Leu Lys Ala Gln Gly Ala Ser Leu
405 410 415Gln Gln Lys Leu Asn Glu Leu Tyr Arg Arg Tyr Gly Tyr Phe Ala Ser
420 425 430Ser Gln Ile Ala Val Arg Thr Ser Ser Pro Arg Glu Leu Val Asp His
435 440 445Trp Ile Ala His Pro Gln Gln Glu Leu Ile Gly Val Ser Val Thr Pro
450 455 460His Ile Leu Pro Glu Lys Gln Gly Ile Ala Leu His Gly Gln Val Gly465 470 475 480His Val His Ile Arg Ala Ile Gly Arg Val Ser Gly Thr Glu Ala Lys
485 490 495Ala Lys Leu Tyr Leu Glu Val Gly Gln Ala Ser Ser His Asp Glu Ala
500 505 510Ala Gln Leu Leu His Gln Leu Glu Asp Glu Val Gln Ser Trp Leu Ser
515 520 525<210>35<211>536<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(513)<223>RXA00511<400>35gag ctg cgc aag aac acc ctc gtg ggc act gtc atg agc aac ctg gga 48Glu Leu Arg Lys Asn Thr Leu Val Gly Thr Val Met Ser Asn Leu Gly1 5 10 15ttg aag att gct atg gat gaa gcc gga att aca ctg cgt acc acc aag 96Leu Lys Ile Ala Met Asp Glu Ala Gly Ile Thr Leu Arg Thr Thr Lys
20 25 30gta gga gac cgc tac gtg ctg gaa gac ctc aat gca ggt gga ttc agc 144Val Gly Asp Arg Tyr Val Leu Glu Asp Leu Asn Ala Gly Gly Phe Ser
35 40 45ctg ggc ggc gag caa tct ggc cac att gtt ctt cca gat cat ggc acc 192Leu Gly Gly Glu Gln Ser Gly His Ile Val Leu Pro Asp His Gly Thr
50 55 60act ggc gat gga act ttg act ggt ctt tcc atc atg gcg cgc atg gct 240Thr Gly Asp Gly Thr Leu Thr Gly Leu Ser Ile Met Ala Arg Met Ala65 70 75 80gaa acc gga aag tcc ttg ggc gag ttg gca caa gct atg acg gtg ctg 288Glu Thr Gly Lys Ser Leu Gly Glu Leu Ala Gln Ala Met Thr Val Leu
85 90 95cca cag gtt ctg atc aat gtg cca gtt tcg gat aag tcc acc atc gtg 336Pro Gln Val Leu Ile Asn Val Pro Val Ser Asp Lys Ser Thr Ile Val
100 105 110agc cac cca agc gtt gtg gct gcg atc gcg gaa gca gaa gct gag ttg 384Ser His Pro Ser Val Val Ala Ala Ile Ala Glu Ala Glu Ala Glu Leu
115 120 125ggc gcc acc ggt cgc gtt ctt ctt cgt gct tct ggc acc gaa gag ctt 432Gly Ala Thr Gly Arg Val Leu Leu Arg Ala Ser Gly Thr Glu Glu Leu
130 135 140ttc cgc gtg atg gtt gag gct gga gac aag gaa caa gct cgt cgt atc 480Phe Arg Val Met Val Glu Ala Gly Asp Lys Glu Gln Ala Arg Arg Ile145 150 155 160gcg gga cgt ctt gct gca gtg gtt gca gaa gtc taattcactt cagtcacagc 533Ala Gly Arg Leu Ala Ala Val Val Ala Glu Val
165 170gca 536<210>36<211>171<212>PRT<213>谷氨酸棒杆菌<400> 36Glu Leu Arg Lys Asn Thr Leu Val Gly Thr Val Met Ser Asn Leu Gly1 5 10 15Leu Lys Ile Ala Met Asp Glu Ala Gly Ile Thr Leu Arg Thr Thr Lys
20 25 30Val Gly Asp Arg Tyr Val Leu Glu Asp Leu Asn Ala Gly Gly Phe Ser
35 40 45Leu Gly Gly Glu Gln Ser Gly His Ile Val Leu Pro Asp His Gly Thr
50 55 60Thr Gly Asp Gly Thr Leu Thr Gly Leu Ser Ile Met Ala Arg Met Ala65 70 75 80Glu Thr Gly Lys Ser Leu Gly Glu Leu Ala Gln Ala Met Thr Val Leu
85 90 95Pro Gln Val Leu Ile Asn Val Pro Val Ser Asp Lys Ser Thr Ile Val
100 105 110Ser His Pro Ser Val Val Ala Ala Ile Ala Glu Ala Glu Ala Glu Leu
115 120 125Gly Ala Thr Gly Arg Val Leu Leu Arg Ala Ser Gly Thr Glu Glu Leu
130 135 140Phe Arg Val Met Val Glu Ala Gly Asp Lys Glu Gln Ala Arg Arg Ile145 150 155 160Ala Gly Arg Leu Ala Ala Val Val Ala Glu Val
165 170<210>37<211>1497<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1474)<223>RXN01365<400> 37cctgatcagg acgaatcata aggtttgcta ttcggattgg atcctttggc aggggtagga 60ttgcaagcgt tattttgttc cctaacccct tcgaggattt atg cgt acc cgt gaa 115
Met Arg Thr Arg Glu
1 5tct gtc aca gct gta att aag gcg tat gac gtc cgt ggt gtt gtt ggt 163Ser Val Thr Ala Val Ile Lys Ala Tyr Asp Val Arg Gly Val Val Gly
10 15 20gtc gat att gat gct gat ttc att tct gag act ggc gct gcc ttt ggt 211Val Asp Ile Asp Ala Asp Phe Ile Ser Glu Thr Gly Ala Ala Phe Gly
25 30 35cgg ctc atg cgt agt gag ggt gaa acc acc gtt gct att ggc cat gac 259Arg Leu Met Arg Ser Glu Gly Glu Thr Thr Val Ala Ile Gly His Asp
40 45 50atg cgt gat tcc tcc cct gaa ttg gcc aag gcg ttt gcc gat ggc gtg 307Met Arg Asp Ser Ser Pro Glu Leu Ala Lys Ala Phe Ala Asp Gly Val
55 60 65act gca cag ggt ttg gat gtt gtt cat ttg gga ctg act tct act gat 355Thr Ala Gln Gly Leu Asp Val Val His Leu Gly Leu Thr Ser Thr Asp70 75 80 85gag ctg tac ttt gcg tcc gga acc ttg aag tgt gct ggt gcg atg ttt 403Glu Leu Tyr Phe Ala Ser Gly Thr Leu Lys Cys Ala Gly Ala Met Phe
90 95 100act gcg tcg cat aac ccc gct gag tac aac ggc atc aag ttg tgt cgt 451Thr Ala Ser His Asn Pro Ala Glu Tyr Asn Gly Ile Lys Leu Cys Arg
105 110 115gcg ggt gct cgt ccg gtc ggt cag gat tct ggt ttg gcc aac atc att 499Ala Gly Ala Arg Pro Val Gly Gln Asp Ser Gly Leu Ala Asn Ile Ile
120 125 130gat gat ctg gtt gag ggt gtt cca gcg ttt gat ggt gag tca ggt tcg 547Asp Asp Leu Val Glu Gly Val Pro Ala Phe Asp Gly Glu Ser Gly Ser
135 140 145gtt tct gag cag gat ttg ctg agc gca tat gcc gag tac ctc aat gag 595Val Ser Glu Gln Asp Leu Leu Ser Ala Tyr Ala Glu Tyr Leu Asn Glu150 155 160 165ctt gtt gat ctg aag aac atc cgc ccg atg aag gtt gct gtg gat gcg 643Leu Val Asp Leu Lys Asn Ile Arg Pro Met Lys Val Ala Val Asp Ala
170 175 180gca aac ggc atg ggt ggg ttc act gtc cct gag gta ttc aag ggt ctg 691Ala Asn Gly Met Gly Gly Phe Thr Val Pro Glu Val Phe Lys Gly Leu
185 190 195cca ctt gat gtt gcg cca ctg tat ttt gag ctt gac ggc aat ttc ccc 739Pro Leu Asp Val Ala Pro Leu Tyr Phe Glu Leu Asp Gly Asn Phe Pro
200 205 210aac cat gag gcc aat cct ctg gag cct gcc aac ctg gtt gat ttg cag 787Asn His Glu Ala Asn Pro Leu Glu Pro Ala Asn Leu Val Asp Leu Gln
215 220 225aag ttt acc gta gag acc gga tct gat atc ggt ttg gcg ttc gac ggc 835Lys Phe Thr Val Glu Thr Gly Ser Asp Ile Gly Leu Ala Phe Asp Gly230 235 240 245gat gcg gat cgt tgc ttc gtg gtc gat gag aag ggc cag cca gtc agc 883Asp Ala Asp Arg Cys Phe Val Val Asp Glu Lys Gly Gln Pro Val Ser
250 255 260cct tcg gcg atc tgt gcg atc gta gcg gag cgt tac ttg gag aag ctt 931Pro Ser Ala IIe Cys Ala Ile Val Ala Glu Arg Tyr Leu Glu Lys Leu
265 270 275ccg ggt tcc acc atc atc cac aac ctg att acc tct aag gct gtg cct 979Pro Gly Ser Thr Ile Ile His Asn Leu Ile Thr Ser Lys Ala Val Pro
280 285 290gag gtg att gct gaa aac ggt ggc act gcg gtg cgt act cgc gtg ggt 1027Glu Val Ile Ala Glu Asn Gly Gly Thr Ala Val Arg Thr Arg Val Gly
295 300 305cac tcc ttc atc aag gcg aag atg gca gag acc ggt gcg gcc ttt ggt 1075His Ser Phe Ile Lys Ala Lys Met Ala Glu Thr Gly Ala Ala Phe Gly310 315 320 325ggc gag cac tct gcg cac tac tac ttc act gag ttc ttc aat gcg gac 1123Gly Glu His Ser Ala His Tyr Tyr Phe Thr Glu Phe Phe Asn Ala Asp
330 335 340tcc ggc att ttg gct gcg atg cac gtg ctg gct gcg ctg gga agc cag 1171Ser Gly Ile Leu Ala Ala Met His Val Leu Ala Ala Leu Gly Ser Gln
345 350 355gac cag cca ctc agt gag atg atg gct agg tat aac cgg tac gtt gct 1219Asp Gln Pro Leu Ser Glu Met Met Ala Arg Tyr Asn Arg Tyr Val Ala
360 365 370tca ggc gag ttg aac tcc cgt ttg gct aat gca gag gcg cag caa gag 1267Ser Gly Glu Leu Asn Ser Arg Leu Ala Asn Ala Glu Ala Gln Gln Glu
375 380 385cgc acc cag gct gtg ctc gat gcg ttc gct gat cgc acc gag tcc gtg 1315Arg Thr Gln Ala Val Leu Asp Ala Phe Ala Asp Arg Thr Glu Ser Val390 395 400 405gac acc ctt gac ggc gtg act gtg gaa ctc aag gac acc tcc gcg tgg 1363Asp Thr Leu Asp Gly Val Thr Val Glu Leu Lys Asp Thr Ser Ala Trp
410 415 420ttc aac gtg cgt gcg tcc aac acc gag ccg ctg ctt cgc ctc aat gtt 1411Phe Asn Val Arg Ala Ser Asn Thr Glu Pro Leu Leu Arg Leu Asn Val
425 430 435gaa gct gca tcg aag gaa gaa gtc gat gcg ttg gta gcg gag att cta 1459Glu Ala Ala Ser Lys Glu Glu Val Asp Ala Leu Val Ala Glu Ile Leu
440 445 450ggg att atc cgc gca taatcccatt ttccggcggg cat 1497Gly Ile Ile Arg Ala
455<210>38<211>458<212>PRT<213>谷氨酸棒杆菌<400> 38Met Arg Thr Arg Glu Ser Val Thr Ala Val Ile Lys Ala Tyr Asp Val1 5 10 15Arg Gly Val Val Gly Val Asp Ile Asp Ala Asp Phe Ile Ser Glu Thr
20 25 30Gly Ala Ala Phe Gly Arg Leu Met Arg Ser Glu Gly Glu Thr Thr Val
35 40 45Ala Ile Gly His Asp Met Arg Asp Ser Ser Pro Glu Leu Ala Lys Ala
50 55 60Phe Ala Asp Gly Val Thr Ala Gln Gly Leu Asp Val Val His Leu Gly65 70 75 80Leu Thr Ser Thr Asp Glu Leu Tyr Phe Ala Ser Gly Thr Leu Lys Cys
85 90 95Ala Gly Ala Met Phe Thr Ala Ser His Asn Pro Ala Glu Tyr Asn Gly
100 105 110Ile Lys Leu Cys Arg Ala Gly Ala Arg Pro Val Gly Gln Asp Ser Gly
115 120 125Leu Ala Asn Ile Ile Asp Asp Leu Val Glu Gly Val Pro Ala Phe Asp
130 135 140Gly Glu Ser Gly Ser Val Ser Glu Gln Asp Leu Leu Ser Ala Tyr Ala145 150 155 160Glu Tyr Leu Asn Glu Leu Val Asp Leu Lys Asn Ile Arg Pro Met Lys
165 170 175Val Ala Val Asp Ala Ala Asn Gly Met Gly Gly Phe Thr Val Pro Glu
180 185 190Val Phe Lys Gly Leu Pro Leu Asp Val Ala Pro Leu Tyr Phe Glu Leu
195 200 205Asp Gly Asn Phe Pro Asn His Glu Ala Asn Pro Leu Glu Pro Ala Asn
210 215 220Leu Val Asp Leu Gln Lys Phe Thr Val Glu Thr Gly Ser Asp Ile Gly225 230 235 240Leu Ala Phe Asp Gly Asp Ala Asp Arg Cys Phe Val Val Asp Glu Lys
245 250 255Gly Gln Pro Val Ser Pro Ser Ala Ile Cys Ala Ile Val Ala Glu Arg
260 265 270Tyr Leu Glu Lys Leu Pro Gly Ser Thr Ile Ile His Asn Leu Ile Thr
275 280 285Ser Lys Ala Val Pro Glu Val Ile Ala Glu Asn Gly Gly Thr Ala Val
290 295 300Arg Thr Arg Val Gly His Ser Phe Ile Lys Ala Lys Met Ala Glu Thr305 310 315 320Gly Ala Ala Phe Gly Gly Glu His Ser Ala His Tyr Tyr Phe Thr Glu
325 330 335Phe Phe Asn Ala Asp Ser Gly Ile Leu Ala Ala Met His Val Leu Ala
340 345 350Ala Leu Gly Ser Gln Asp Gln Pro Leu Ser Glu Met Met Ala Arg Tyr
355 360 365Asn Arg Tyr Val Ala Ser Gly Glu Leu Asn Ser Arg Leu Ala Asn Ala
370 375 380Glu Ala Gln Gln Glu Arg Thr Gln Ala Val Leu Asp Ala Phe Ala Asp385 390 395 400Arg Thr Glu Ser Val Asp Thr Leu Asp Gly Val Thr Val Glu Leu Lys
405 410 415Asp Thr Ser Ala Trp Phe Asn Val Arg Ala Ser Asn Thr Glu Pro Leu
420 425 430Leu Arg Leu Asn Val Glu Ala Ala Ser Lys Glu Glu Val Asp Ala Leu
435 440 445Val Ala Glu Ile Leu Gly Ile Ile Arg Ala
450 455<210>39<211>994<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(994)<223>FRXA0l365<400>39cctgatcagg acgaatcata aggtttgcta ttcggattgg atcctttggc aggggtagga 60ttgcaagcgt tattttgttc cctaacccct tcgaggattt atg cgt acc cgt gaa 115
Met Arg Thr Arg Glu
1 5tct gtc aca gct gta att aag gcg tat gac gtc cgt ggt gtt gtt ggt 163Ser Val Thr Ala Val Ile Lys Ala Tyr Asp Val Arg Gly Val Val Gly
10 15 20gtc gat att gat gct gat ttc att tct gag act ggc gct gcc ttt ggt 211Val Asp Ile Asp Ala Asp Phe Ile Ser Glu Thr Gly Ala Ala Phe Gly
25 30 35cgg ctc atg cgt agt gag ggt gaa acc acc gtt gct att ggc cat gac 259Arg Leu Met Arg Ser Glu Gly Glu Thr Thr Val Ala Ile Gly His Asp
40 45 50atg cgt gat tcc tcc cct gaa ttg gcc aag gcg ttt gcc gat ggc gtg 307Met Arg Asp Ser Ser Pro Glu Leu Ala Lys Ala Phe Ala Asp Gly Val
55 60 65act gca cag ggt ttg gat gtt gtt cat ttg gga ctg act tct act gat 355Thr Ala Gln Gly Leu Asp Val Val His Leu Gly Leu Thr Ser Thr Asp70 75 80 85gag ctg tac ttt gcg tcc gga acc ttg aag tgt gct ggt gcg atg ttt 403Glu Leu Tyr Phe Ala Ser Gly Thr Leu Lys Cys Ala Gly Ala Met Phe
90 95 100act gcg tcg cat aac ccc gct gag tac aac ggc atc aag ttg tgt cgt 451Thr Ala Ser His Asn Pro Ala Glu Tyr Asn Gly Ile Lys Leu Cys Arg
105 110 115gcg ggt gct cgt ccg gtc ggt cag gat tct ggt ttg gcc aac atc att 499Ala Gly Ala Arg Pro Val Gly Gln Asp Ser Gly Leu Ala Asn Ile Ile
120 125 130gat gat ctg gtt gag ggt gtt cca gcg ttt gat ggt gag tca ggt tcg 547Asp Asp Leu Val Glu Gly Val Pro Ala Phe Asp Gly Glu Ser Gly Ser
135 140 145gtt tct gag cag gat ttg ctg agc gca tat gcc gag tac ctc aat gag 595Val Ser Glu Gln Asp Leu Leu Ser Ala Tyr Ala Glu Tyr Leu Asn Glu150 155 160 165ctt gtt gat ctg aag aac atc cgc ccg atg aag gtt gct gtg gat gcg 643Leu Val Asp Leu Lys Asn Ile Arg Pro Met Lys Val Ala Val Asp Ala
170 175 180gca aac ggc atg ggt ggg ttc act gtc cct gag gta ttc aag ggt ctg 691Ala Asn Gly Met Gly Gly Phe Thr Val Pro Glu Val Phe Lys Gly Leu
185 190 195cca ctt gat gtt gcg cca ctg tat ttt gag ctt gac ggc aat ttc ccc 739Pro Leu Asp Val Ala Pro Leu Tyr Phe Glu Leu Asp Gly Asn Phe Pro
200 205 210aac cat gag gcc aat cct ctg gag cct gcc aac ctg gtt gat ttg cag 787Asn His Glu Ala Asn Pro Leu Glu Pro Ala Asn Leu Val Asp Leu Gln
215 220 225aag ttt acc gta gag acc gga tct gat atc ggt ttg gcg ttc gac ggc 835Lys Phe Thr Val Glu Thr Gly Ser Asp Ile Gly Leu Ala Phe Asp Gly230 235 240 245gat gcg gat cgt tgc ttc gtg gtc gat gag aag ggc cag cca gtc agc 883Asp Ala Asp Arg Cys Phe Val Val Asp Glu Lys Gly Gln Pro Val Ser
250 255 260cct tcg gcg atc tgt gcg atc gta gcg gag cgt tac ttg gag aag ctt 931Pro Ser Ala Ile Cys Ala Ile Val Ala Glu Arg Tyr Leu Glu Lys Leu
265 270 275ccg ggt tcc acc atc atc cac aac ctg att acc tct aag gct gtg cct 979Pro Gly Ser Thr Ile Ile His Asn Leu Ile Thr Ser Lys Ala Val Pro
280 285 290gag gtg att gct gaa 994Glu Val Ile Ala Glu
295<210>40<211>298<212>PRT<213>谷氨酸棒杆菌<400> 40Met Arg Thr Arg Glu Ser Val Thr Ala Val Ile Lys Ala Tyr Asp Val1 5 10 15Arg Gly Val Val Gly Val Asp Ile Asp Ala Asp Phe Ile Ser Glu Thr
20 25 30Gly Ala Ala Phe Gly Arg Leu Met Arg Ser Glu Gly Glu Thr Thr Val
35 40 45Ala Ile Gly His Asp Met Arg Asp Ser Ser Pro Glu Leu Ala Lys Ala
50 55 60Phe Ala Asp Gly Val Thr Ala Gln Gly Leu Asp Val Val His Leu Gly65 70 75 80Leu Thr Ser Thr Asp Glu Leu Tyr Phe Ala Ser Gly Thr Leu Lys Cys
85 90 95Ala Gly Ala Met Phe Thr Ala Ser His Asn Pro Ala Glu Tyr Asn Gly
100 105 110Ile Lys Leu Cys Arg Ala Gly Ala Arg Pro Val Gly Gln Asp Ser Gly
115 120 125Leu Ala Asn Ile Ile Asp Asp Leu Val Glu Gly Val Pro Ala Phe Asp
130 135 140Gly Glu Ser Gly Ser Val Ser Glu Gln Asp Leu Leu Ser Ala Tyr Ala145 150 155 160Glu Tyr Leu Asn Glu Leu Val Asp Leu Lys Asn Ile Arg Pro Met Lys
165 170 175Val Ala Val Asp Ala Ala Asn Gly Met Gly Gly Phe Thr Val Pro Glu
180 185 190Val Phe Lys Gly Leu Pro Leu Asp Val Ala Pro Leu Tyr Phe Glu Leu
195 200 205Asp Gly Asn Phe Pro Asn His Glu Ala Asn Pro Leu Glu Pro Ala Asn
210 215 220Leu Val Asp Leu Gln Lys Phe Thr Val Glu Thr Gly Ser Asp Ile Gly225 230 235 240Leu Ala Phe Asp Gly Asp Ala Asp Arg Cys Phe Val Val Asp Glu Lys
245 250 255Gly Gln Pro Val Ser Pro Ser Ala Ile Cys Ala Ile Val Ala Glu Arg
260 265 270Tyr Leu Glu Lys Leu Pro Gly Ser Thr Ile Ile His Asn Leu Ile Thr
276 280 285Ser Lys Ala Val Pro Glu Val Ile Ala Glu
290 295<210>41<211>1743<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1720)<223>RXA00098<400>41taaatttgtc gtgtttccca ctttgaacac tcttcgatgc gcttggccac aaaagcaagc 60taacctgaag atgttattta acgacaataa aggagttttc atg gcg gac att tcg 115
Met Ala Asp lle Ser
1 5acc acc cag gtt tgg caa gac ctg acc gat cat tac tca aac ttc cag 163Thr Thr Gln Val Trp Gln Asp Leu Thr Asp His Tyr Ser Asn Phe Gln
10 15 20gca acc act ctg cgt gaa ctt ttc aag gaa gaa aac cgc gcc gag aag 211Ala Thr Thr Leu Arg Glu Leu Phe Lys Glu Glu Asn Arg Ala Glu Lys
25 30 35tac acc ttc tcc gcg gct ggc ctc cac gtc gac ctg tcg aag aat ctg 259Tyr Thr Phe Ser Ala Ala Gly Leu His Val Asp Leu Ser Lys Asn Leu
40 45 50ctt gac gac gcc acc ctc acc aag ctc ctt gca ctg acc gaa gaa tct 307Leu Asp Asp Ala Thr Leu Thr Lys Leu Leu Ala Leu Thr Glu Glu Ser
55 60 65ggc ctt cgc gaa cgc att gac gcg atg ttt gcc ggt gaa cac ctc aac 355Gly Leu Arg Glu Arg Ile Asp Ala Met Phe Ala Gly Glu His Leu Asn70 75 80 85aac acc gaa gac cgc gct gtc ctc cac acc gcg ctg cgc ctt cct gcc 403Asn Thr Glu Asp Arg Ala Val Leu His Thr Ala Leu Arg Leu Pro Ala
90 95 100gaa gct gat ctg tca gta gat ggc caa gat gtt gct gct gat gtc cac 451Glu Ala Asp Leu Ser Val Asp Gly Gln Asp Val Ala Ala Asp Val His
105 110 115gaa gtt ttg gga cgc atg cgt gac ttc gct act gcg ctg cgc tca ggc 499Glu Val Leu Gly Arg Met Arg Asp Phe Ala Thr Ala Leu Arg Ser Gly
120 125 130aac tgg ttg gga cac acc ggc cac acg atc aag aag atc gtc aac att 547Asn Trp Leu Gly His Thr Gly His Thr Ile Lys Lys Ile Val Asn Ile
135 140 145ggt atc ggt ggc tct gac ctc gga cca gcc atg gct acg aag gct ctg 595Gly Ile Gly Gly Ser Asp Leu Gly Pro Ala Met Ala Thr Lys Ala Leu150 155 160 165cgt gca tac gcg acc gct ggt atc tca gca gaa ttc gtc tcc aac gtc 643Arg Ala Tyr Ala Thr Ala Gly Ile Ser Ala Glu Phe Val Ser Asn Val
170 175 180gac cca gca gac ctc gtt tct gtg ttg gaa gac ctc gat gca gaa tcc 691Asp Pro Ala Asp Leu Val Ser Val Leu Glu Asp Leu Asp Ala Glu Ser
185 190 195aca ttg ttc gtg atc gct tcg aaa act ttc acc acc cag gag acg ctg 739Thr Leu Phe Val Ile Ala Ser Lys Thr Phe Thr Thr Gln Glu Thr Leu
200 205 210tcc aac gct cgt gca gct cgt gct tgg ctg gta gag aag ctc ggt gaa 787Ser Asn Ala Arg Ala Ala Arg Ala Trp Leu Val Glu Lys Leu Gly Glu
215 220 225gag gct gtc gcg aag cac ttc gtc gca gtg tcc acc aat gct gaa aag 835Glu Ala Val Ala Lys His Phe Val Ala Val Ser Thr Asn Ala Glu Lys230 235 240 245gtc gca gag ttc ggt atc gac acg gac aac atg ttc ggc ttc tgg gac 883Val Ala Glu Phe Gly Ile Asp Thr Asp Asn Met Phe Gly Phe Trp Asp
250 255 260tgg gtc gga ggt cgt tac tcc gtg gac tcc gca gtt ggt ctt tcc ctc 931Trp Val Gly Gly Arg Tyr Ser Val Asp Ser Ala Val Gly Leu Ser Leu
265 270 275atg gca gtg atc ggc cct cgc gac ttc atg cgt ttc ctc ggt gga ttc 979Met Ala Val Ile Gly Pro Arg Asp Phe Met Arg Phe Leu Gly Gly Phe
280 285 290cac gcg atg gat gaa cacttc cgc acc acc aag ttc gaa gag aac gtt 1027His Ala Met Asp Glu His Phe Arg Thr Thr Lys Phe Glu Glu Asn Val
295 300 305cca atc ttg atg gct ctg ctc ggt gtc tgg tac tcc gat ttc tat ggt 1075Pro Ile Leu Met Ala Leu Leu Gly Val Trp Tyr Ser Asp Phe Tyr Gly310 315 320 325gca gaa acc cac gct gtc cta cct tat tcc gag gat ctc agc cgt ttt 1123Ala Glu Thr His Ala Val Leu Pro Tyr Ser Glu Asp Leu Ser Arg Phe
330 335 340gct gct tac ctc cag cag ctg acc atg gaa tca aat ggc aag tca gtc 1171Ala Ala Tyr Leu Gln Gln Leu Thr Met Glu Ser Asn Gly Lys Ser Val
345 350 355cac cgc gac ggc tcc cct gtt tcc act ggc act ggc gaa att tac tgg 1219His Arg Asp Gly Ser Pro Val Ser Thr Gly Thr Gly Glu Ile Tyr Trp
360 365 370ggt gag cct ggc aca aat ggc cag cac gct ttc ttc cag ctg atc cac 1267Gly Glu Pro Gly Thr Asn Gly Gln His Ala Phe Phe Gln Leu Ile His
375 380 385cag ggc act cgc ctt gtt cca gct gat ttc att ggt ttc gct cgt cca 1315Gln Gly Thr Arg Leu Val Pro Ala Asp Phe Ile Gly Phe Ala Arg Pro390 395 400 405aag cag gat ctt cct gcc ggt gag cgc acc atg cat gac ctt ttg atg 1363Lys Gln Asp Leu Pro Ala Gly Glu Arg Thr Met His Asp Leu Leu Met
410 415 420agc aac ttc ttc gca cag acc aag gtt ttg gct ttc ggt aag aac gct 1411Ser Asn Phe Phe Ala Gln Thr Lys Val Leu Ala Phe Gly Lys Asn Ala
425 430 435gaa gag atc gct gcg gaa ggt gtc gca cct gag ctg gtc aac cac aag 1459Glu Glu Ile Ala Ala Glu Gly Val Ala Pro Glu Leu Val Asn His Lys
440 445 450gtc atg cca ggt aat cgc cca acc acc acc att ttg gcg gag gaa ctt 1507Val Met Pro Gly Asn Arg Pro Thr Thr Thr Ile Leu Ala Glu Glu Leu
455 460 465acc cct tct att ctc ggt gcg ttg atc gct ttg tac gaa cac atc gtg 1555Thr Pro Ser Ile Leu Gly Ala Leu Ile Ala Leu Tyr Glu His Ile Val470 475 480 485atg gtt cag ggc gtg att tgg gac atc aac tcc ttc gac caa tgg ggt 1603Met Val Gln Gly Val Ile Trp Asp Ile Asn Ser Phe Asp Gln Trp Gly
490 495 500gtt gaa ctg ggc aaa cag cag gca aat gac ctc gct ccg gct gtc tct 1651Val Glu Leu Gly Lys Gln Gln Ala Asn Asp Leu Ala Pro Ala Val Ser
505 510 515ggt gaa gag gat gtt gac tcg gga gat tct tcc act gat tca ctg att 1699Gly Glu Glu Asp Val Asp Ser Gly Asp Ser Ser Thr Asp Ser Leu Ile
520 525 530aag tgg tac cgc gca aat agg tagtcgcttg cttatagggt cag 1743Lys Trp Tyr Arg Ala Asn Arg
535 540<210>42<211>540<212>PRT<213>谷氨酸棒杆菌<400> 42Met Ala Asp Ile Ser Thr Thr Gln Val Trp Gln Asp Leu Thr Asp His1 5 10 15Tyr Ser Asn Phe Gln Ala Thr Thr Leu Arg Glu Leu Phe Lys Glu Glu
20 25 30Asn Arg Ala Glu Lys Tyr Thr Phe Ser Ala Ala Gly Leu His Val Asp
35 40 45Leu Ser Lys Asn Leu Leu Asp Asp Ala Thr Leu Thr Lys Leu Leu Ala
50 55 60Leu Thr Glu Glu Ser Gly Leu Arg Glu Arg Ile Asp Ala Met Phe Ala65 70 75 80Gly Glu His Leu Asn Asn Thr Glu Asp Arg Ala Val Leu His Thr Ala
85 90 95Leu Arg Leu Pro Ala Glu Ala Asp Leu Ser Val Asp Gly Gln Asp Val
100 105 110Ala Ala Asp Val His Glu Val Leu Gly Arg Met Arg Asp Phe Ala Thr
115 120 125Ala Leu Arg Ser Gly Asn Trp Leu Gly His Thr Gly His Thr Ile Lys
130 135 140Lys Ile Val Asn Ile Gly Ile Gly Gly Ser Asp Leu Gly Pro Ala Met145 150 155 160Ala Thr Lys Ala Leu Arg Ala Tyr Ala Thr Ala Gly Ile Ser Ala Glu
165 170 175Phe Val Ser Asn Val Asp Pro Ala Asp Leu Val Ser Val Leu Glu Asp
180 185 190Leu Asp Ala Glu Ser Thr Leu Phe Val Ile Ala Ser Lys Thr Phe Thr
195 200 205Thr Gln Glu Thr Leu Ser Asn Ala Arg Ala Ala Arg Ala Trp Leu Val
210 215 220Glu Lys Leu Gly Glu Glu Ala Val Ala Lys His Phe Val Ala Val Ser225 230 235 240Thr Asn Ala Glu Lys Val Ala Glu Phe Gly Ile Asp Thr Asp Asn Met
245 250 255Phe Gly Phe Trp Asp Trp Val Gly Gly Arg Tyr Ser Val Asp Ser Ala
260 265 270Val Gly Leu Ser Leu Met Ala Val Ile Gly Pro Arg Asp Phe Met Arg
275 280 285Phe Leu Gly Gly Phe His Ala Met Asp Glu His Phe Arg Thr Thr Lys
290 295 300Phe Glu Glu Asn Val Pro Ile Leu Met Ala Leu Leu Gly Val Trp Tyr305 310 315 320Ser Asp Phe Tyr Gly Ala Glu Thr His Ala Val Leu Pro Tyr Ser Glu
325 330 335Asp Leu Ser Arg Phe Ala Ala Tyr Leu Gln Gln Leu Thr Met Glu Ser
340 345 350Asn Gly Lys Ser Val His Arg Asp Gly Ser Pro Val Ser Thr Gly Thr
355 360 365Gly Glu Ile Tyr Trp Gly Glu Pro Gly Thr Asn Gly Gln His Ala Phe
370 375 380Phe Gln Leu Ile His Gln Gly Thr Arg Leu Val Pro Ala Asp Phe Ile385 390 395 400Gly Phe Ala Arg Pro Lys Gln Asp Leu Pro Ala Gly Glu Arg Thr Met
405 410 415His Asp Leu Leu Met Ser Asn Phe Phe Ala Gln Thr Lys Val Leu Ala
420 425 430Phe Gly Lys Asn Ala Glu Glu Ile Ala Ala Glu Gly Val Ala Pro Glu
435 440 445Leu Val Asn His Lys Val Met Pro Gly Asn Arg Pro Thr Thr Thr Ile
450 455 460Leu Ala Glu Glu Leu Thr Pro Ser Ile Leu Gly Ala Leu Ile Ala Leu465 470 475 480Tyr Glu His Ile Val Met Val Gln Gly Val Ile Trp Asp Ile Asn Ser
485 490 495Phe Asp Gln Trp Gly Val Glu Leu Gly Lys Gln Gln Ala Asn Asp Leu
500 505 510Ala Pro Ala Val Ser Gly Glu Glu Asp Val Asp Ser Gly Asp Ser Ser
515 520 525Thr Asp Ser Leu Ile Lys Trp Tyr Arg Ala Asn Arg
530 535 540<210>43<211>630<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(630)<223>RXA01989<400>43gtt aaa tca att cac aaa aca att cat gaa ggt act ggt gca ggt agt 48Val Lys Ser Ile His Lys Thr Ile His Glu Gly Thr Gly Ala Gly Ser1 5 10 15gac ttc tta ggc tgg gtt gat tta cca gtt gat tac gac aaa gaa gaa 96Asp Phe Leu Gly Trp Val Asp Leu Pro Val Asp Tyr Asp Lys Glu Glu
20 25 30ttt tca aga att gtt gaa gca tca aaa cgc att aaa gaa aat tct gat 144Phe Ser Arg Ile Val Glu Ala Ser Lys Arg Ile Lys Glu Asn Ser Asp
35 40 45gtt tta gta gtc atc ggt att ggt ggt tct tac tta ggt gca cgt gca 192Val Leu Val Val Ile Gly Ile Gly Gly Ser Tyr Leu Gly Ala Arg Ala
50 55 60gca atc gaa atg tta acg tca tca ttt aga aac agc aat gaa tac cct 240Ala Ile Glu Met Leu Thr Ser Ser Phe Arg Asn Ser Asn Glu Tyr Pro65 70 75 80gaa att gta ttt gtt ggt aat cac tta tca tca aca tat acg aaa gag 288Glu Ile Val Phe Val Gly Asn His Leu Ser Ser Thr Tyr Thr Lys Glu
85 90 95tta gtt gat tat tta gca gac aaa gat ttc tct gta aac gtt att tct 336Leu Val Asp Tyr Leu Ala Asp Lys Asp Phe Ser Val Asn Val Ile Ser
100 105 110aaa tct ggt aca act aca gaa cca gca gtt gca ttt aga ttg ttc aaa 384Lys Ser Gly Thr Thr Thr Glu Pro Ala Val Ala Phe Arg Leu Phe Lys
115 120 125caa tta gtt gaa gaa aga tac ggt aaa gaa gaa gca caa aaa cgt ata 432Gln Leu Val Glu Glu Arg Tyr Gly Lys Glu Glu Ala Gln Lys Arg Ile
130 135 140ttt gca aca acg gat aaa gaa aaa ggt gct tta aaa cag ttg gct aca 480Phe Ala Thr Thr Asp Lys Glu Lys Gly Ala Leu Lys Gln Leu Ala Thr145 150 155 160aac gaa ggt tat gaa acg ttt atc gta cct gat gat gta ggt gga aga 528Asn Glu Gly Tyr Glu Thr Phe Ile Val Pro Asp Asp Val Gly Gly Arg
165 170 175tat tct gtt tta aca gca gta gga tta tta cca att gca aca gct gga 576Tyr Ser Val Leu Thr Ala Val Gly Leu Leu Pro Ile Ala Thr Ala Gly
180 185 190att aac atc gaa gct atg atg att ggt gct gca aaa gca cgt gaa gaa 624Ile Asn Ile Glu Ala Met Met Ile Gly Ala Ala Lys Ala Arg Glu Glu
195 200 205tta tct 630Leu Ser
210<210>44<211>210<212>PRT<213>谷氨酸棒杆菌<400>44Val Lys Ser Ile His Lys Thr Ile His Glu Gly Thr Gly Ala Gly Ser1 5 10 15Asp Phe Leu Gly Trp Val Asp Leu Pro Val Asp Tyr Asp Lys Glu Glu
20 25 30Phe Ser Arg Ile Val Glu Ala Ser Lys Arg Ile Lys Glu Asn Ser Asp
35 40 45Val Leu Val Val Ile Gly Ile Gly Gly Ser Tyr Leu Gly Ala Arg Ala
50 55 60Ala Ile Glu Met Leu Thr Ser Ser Phe Arg Asn Ser Asn Glu Tyr Pro65 70 75 80Glu Ile Val Phe Val Gly Asn His Leu Ser Ser Thr Tyr Thr Lys Glu
85 90 95Leu Val Asp Tyr Leu Ala Asp Lys Asp Phe Ser Val Asn Val Ile Ser
100 105 110Lys Ser Gly Thr Thr Thr Glu Pro Ala Val Ala Phe Arg Leu Phe Lys
115 120 125Gln Leu Val Glu Glu Arg Tyr Gly Lys Glu Glu Ala Gln Lys Arg Ile
130 135 140Phe Ala Thr Thr Asp Lys Glu Lys Gly Ala Leu Lys Gln Leu Ala Thr145 150 155 160Asn Glu Gly Tyr Glu Thr Phe Ile Val Pro Asp Asp Val Gly Gly Arg
165 170 175Tyr Ser Val Leu Thr Ala Val Gly Leu Leu Pro Ile Ala Thr Ala Gly
180 185 190Ile Asn Ile Glu Ala Met Met Ile Gly Ala Ala Lys Ala Arg Glu Glu
195 200 205Leu Ser
210<210>45<211>1269<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1246)<223>RXA00340<400>45cggtatctcc gacatccgca acacccccaa agatgaggtt ccacagtgcc cagaatgtgg 60ctcttacctc atcactgaca tctcttagaa agaccaccca gtg aaa tta gtc atc 115
Val Lys Leu Val Ile
1 5gag gcc gac ggc ggc tcc cgc gga aac ccc ggc gtc gcc ggc tcc ggc 163Glu Ala Asp Gly Gly Ser Arg Gly Asn Pro Gly Val Ala Gly Ser Gly
10 15 20acc gtg gtg tac tcc gac aac aaa gca gaa gtt ctc aaa gaa atc gcc 211Thr Val Val Tyr Ser Asp Asn Lys Ala Glu Val Leu Lys Glu Ile Ala
25 30 35tat gtt gtc gga aca aaa gcc acc aac aac gtc gcc gaa tac cgc gga 259Tyr Val Val Gly Thr Lys Ala Thr Asn Asn Val Ala Glu Tyr Arg Gly
40 45 50cta ctc gaa ggc ctc aaa gca gcc cgc gag ctc ggc gct acc tcc gtg 307Leu Leu Glu Gly Leu Lys Ala Ala Arg Glu Leu Gly Ala Thr Ser Val
55 60 65gat gtc tac atg gac tcc aaa ctt gtc gtt gaa caa atg tcc ggc cgg 355Asp Val Tyr Met Asp Ser Lys Leu Val Val Glu Gln Met Ser Gly Arg70 75 80 85tgg aaa atc aaa cac ccc gac atg aaa gtt cta gcg atc gaa gcc aag 403Trp Lys Ile Lys His Pro Asp Met Lys Val Leu Ala Ile Glu Ala Lys
90 95 100gag att gct tcc gaa atc ggg tcc gtt tct tat acg tgg att ccg cgt 451Glu Ile Ala Ser Glu Ile Gly Ser Val Ser Tyr Thr Trp Ile Pro Arg
105 110 115gag aaa aac aaa cga gct gac gca ttg tcc aac gtg gcg atg gat gct 499Glu Lys Asn Lys Arg Ala Asp Ala Leu Ser Asn Val Ala Met Asp Ala
120 125 130gca gcg gca ggt aag ccg gta ggt gtt gta ggg gat tct gct tct gta 547Ala Ala Ala Gly Lys Pro Val Gly Val Val Gly Asp Ser Ala Ser Val
135 140 145tct tct gct tct tcg gtt gcg ggc tca gag aaa gaa gac ctc aac tgc 595Ser Ser Ala Ser Ser Val Ala Gly Ser Glu Lys Glu Asp Leu Asn Cys150 155 160 165acc gaa acc aaa ccc acc aac tgg aac ggc gca acc aca gat ccc act 643Thr Glu Thr Lys Pro Thr Asn Trp Asn Gly Ala Thr Thr Asp Pro Thr
170 175 180cgt ttc ttg ttg ctt cgc cac ggc caa act gct atg tca gtg gca cgc 691Arg Phe Leu Leu Leu Arg His Gly Gln Thr Ala Met Ser Val Ala Arg
185 190 195ctt tac tcc ggt agg tcc aac cca gag ctg tct gaa ctt ggt gaa aaa 739Leu Tyr Ser Gly Arg Ser Asn Pro Glu Leu Ser Glu Leu Gly Glu Lys
200 205 210caa gca gca gcg gca gca cga cga ctc gct caa acc ggt ggc atc gac 787Gln Ala Ala Ala Ala Ala Arg Arg Leu Ala Gln Thr Gly Gly Ile Asp
215 220 225gct att gtg agt tct ccg ctc acc cgc acg atg caa acc gca gaa gca 835Ala Ile Val Ser Ser Pro Leu Thr Arg Thr Met Gln Thr Ala Glu Ala230 235 240 245gca gcg gcc gca ctg gga atg aaa gta cgt gtt atc gat gat ctc atc 883Ala Ala Ala Ala Leu Gly Met Lys Val Arg Val Ile Asp Asp Leu Ile
250 255 260gaa act gac ttt gga ctg tgg gat gga aaa tca ttt tca gaa gcc cac 931Glu Thr Asp Phe Gly Leu Trp Asp Gly Lys Ser Phe Ser Glu Ala His
265 270 275gaa caa gat cca gaa ctg cac acc aag tgg ctc act gac tca tct gta 979Glu Gln Asp Pro Glu Leu His Thr Lys Trp Leu Thr Asp Ser Ser Val
280 285 290gcc cca ccc ggt ggt gag tcc ctg cag acg gtt aat cga cgt gtg aaa 1027Ala Pro Pro Gly Gly Glu Ser Leu Gln Thr Val Asn Arg Arg Val Lys
295 300 305aag gct cgt gaa agc ctc caa cgc gaa tac ggt gca gcg aat gtt ttg 1075Lys Ala Arg Glu Ser Leu Gln Arg Glu Tyr Gly Ala Ala Asn Val Leu310 315 320 325gtg gtc agc cac gtc acc cca atc aaa gcc atc atg agg caa gca ttg 1123Val Val Ser His Val Thr Pro Ile Lys Ala Ile Met Arg Gln Ala Leu
330 335 340gac gca ggc cca tcc ttc ttt cag aag gca cac ctt gac ttg gcg tcg 1171Asp Ala Gly Pro Ser Phe Phe Gln Lys Ala His Leu Asp Leu Ala Ser
345 350 355ctg tcg atc gca gag ttt tac gaa gac ggc cca acc tgc gta aga ctg 1219Leu Ser Ile Ala Glu Phe Tyr Glu Asp Gly Pro Thr Cys Val Arg Leu
360 365 370ttc aac gac acc tca cac ctg gaa gcg tgacgacagt ctgacggaag 1266Phe Asn Asp Thr Ser His Leu Glu Ala
375 380ctc 1269<210>46<211>382<212>PRT<213>谷氨酸棒杆菌<400> 46Val Lys Leu Val Ile Glu Ala Asp Gly Gly Ser Arg Gly Asn Pro Gly1 5 10 15Val Ala Gly Ser Gly Thr Val Val Tyr Ser Asp Asn Lys Ala Glu Val
20 25 30Leu Lys Glu Ile Ala Tyr Val Val Gly Thr Lys Ala Thr Asn Asn Val
35 40 45Ala Glu Tyr Arg Gly Leu Leu Glu Gly Leu Lys Ala Ala Arg Glu Leu
50 55 60Gly Ala Thr Ser Val Asp Val Tyr Met Asp Ser Lys Leu Val Val Glu65 70 75 80Gln Met Ser Gly Arg Trp Lys Ile Lys His Pro Asp Met Lys Val Leu
85 90 95Ala Ile Glu Ala Lys Glu Ile Ala Ser Glu Ile Gly Ser Val Ser Tyr
100 105 110Thr Trp Ile Pro Arg Glu Lys Asn Lys Arg Ala Asp Ala Leu Ser Asn
115 120 125Val Ala Met Asp Ala Ala Ala Ala Gly Lys Pro Val Gly Val Val Gly
130 135 140Asp Ser Ala Ser Val Ser Ser Ala Ser Ser Val Ala Gly Ser Glu Lys145 150 155 160Glu Asp Leu Asn Cys Thr Glu Thr Lys Pro Thr Asn Trp Asn Gly Ala
165 170 175Thr Thr Asp Pro Thr Arg Phe Leu Leu Leu Arg His Gly Gln Thr Ala
180 185 190Met Ser Val Ala Arg Leu Tyr Ser Gly Arg Ser Asn Pro Glu Leu Ser
195 200 205Glu Leu Gly Glu Lys Gln Ala Ala Ala Ala Ala Arg Arg Leu Ala Gln
210 215 220Thr Gly Gly Ile Asp Ala Ile Val Ser Ser Pro Leu Thr Arg Thr Met225 230 235 240Gln Thr Ala Glu Ala Ala Ala Ala Ala Leu Gly Met Lys Val Arg Val
245 250 255Ile Asp Asp Leu Ile Glu Thr Asp Phe Gly Leu Trp Asp Gly Lys Ser
260 265 270Phe Ser Glu Ala His Glu Gln Asp Pro Glu Leu His Thr Lys Trp Leu
275 280 285Thr Asp Ser Ser Val Ala Pro Pro Gly Gly Glu Ser Leu Gln Thr Val
290 295 300Asn Arg Arg Val Lys Lys Ala Arg Glu Ser Leu Gln Arg Glu Tyr Gly305 310 315 320Ala Ala Asn Val Leu Val Val Ser His Val Thr Pro Ile Lys Ala Ile
325 330 335Met Arg Gln Ala Leu Asp Ala Gly Pro Ser Phe Phe Gln Lys Ala His
340 345 350Leu Asp Leu Ala Ser Leu Ser Ile Ala Glu Phe Tyr Glu Asp Gly Pro
355 360 365Thr Cys Val Arg Leu Phe Asn Asp Thr Ser His Leu Glu Ala
370 375 380<210>47<211>840<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(817)<223>RXA02492<400>47gctgtacaac gacgctattg ccaacgaaaa tgtcgacggt gaaacgcatc acggctaagt 60aaacgcgcgt cgtggaacat aaagtggcaa actagtacct atg act aac gga aaa 115
Met Thr Asn Gly Lys
1 5ttg att ctt ctt cgt cac ggt cag agc gaa tgg aac gca tcc aac cag 163Leu Ile Leu Leu Arg His Gly Gln Ser Glu Trp Asn Ala Ser Asn Gln
10 15 20ttc act gga tgg gtc gac gtc aat ctg acc gaa cag ggt gag gct gag 211Phe Thr Gly Trp Val Asp Val Asn Leu Thr Glu Gln Gly Glu Ala Glu
25 30 35gcc aaa ggc gtc ctc cca ggc gtt gta tac acc tcc ttg ctg cgt cgc 259Ala Lys Gly Val Leu Pro Gly Val Val Tyr Thr Ser Leu Leu Arg Arg
40 45 50gcg atc cgc act gca aac atc gca ctg aac gct gca gac cgc cac tgg 307Ala Ile Arg Thr Ala Asn Ile Ala Leu Asn Ala Ala Asp Arg His Trp
55 60 65atc cca gtg atc cgc gac tgg cgc ctc aac gag cgt cac tac ggc gca 355Ile Pro Val Ile Arg Asp Trp Arg Leu Asn Glu Arg His Tyr Gly Ala70 75 80 85ctg cag ggc ctt gac aag gct gca acc aag gaa aaa tac ggc gac gac 403Leu Gln Gly Leu Asp Lys Ala Ala Thr Lys Glu Lys Tyr Gly Asp Asp
90 95 100cag ttc atg gaa tgg cgc cgc tcc tac gac acc cca cca cca gag ctc 451Gln Phe Met Glu Trp Arg Arg Ser Tyr Asp Thr Pro Pro Pro Glu Leu
105 110 115gcg gat gac gca gag tac tcc cag gca aat gac cct cgt tac gcg gac 499Ala Asp Asp Ala Glu Tyr Ser Gln Ala Asn Asp Pro Arg Tyr Ala Asp
120 125 130ctc gac gta gtt cca cgc acc gaa tgc ctc aag gac gtt gtg gtt cgt 547Leu Asp Val Val Pro Arg Thr Glu Cys Leu Lys Asp Val Val Val Arg
135 140 145ttt gtt cct tac ttc gag gaa gaa atc ctg cca cgc gca aag aag ggc 595Phe Val Pro Tyr Phe Glu Glu Glu Ile Leu Pro Arg Ala Lys Lys Gly150 155 160 165gaa acc gtc ctc atc gca gca cac ggc aac tcc ctg cgt gcg ctg gtt 643Glu Thr Val Leu Ile Ala Ala His Gly Asn Ser Leu Arg Ala Leu Val
170 175 180aag cac ctt gac ggc atc tcc gat gct gat atc gca gag ctc aac atc 691Lys His Leu Asp Gly Ile Ser Asp Ala Asp Ile Ala Glu Leu Asn Ile
185 190 195cca acc ggc atc cca ctg gtc tac gaa atc gcc gaa gac ggt tcc gta 739Pro Thr Gly Ile Pro Leu Val Tyr Glu Ile Ala Glu Asp Gly Ser Val
200 205 210gta aac cca ggc ggc acc tac ctc gat cct gag gca gca gca gcc ggc 787Val Asn Pro Gly Gly Thr Tyr Leu Asp Pro Glu Ala Ala Ala Ala Gly
215 220 225gca gca gca gta gca aac cag ggt aat aag tagctatttg taggtgagca 837Ala Ala Ala Val Ala Asn Gln Gly Asn Lys230 235ctc 840<210>48<211>239<212>PRT<213>谷氨酸棒杆菌<400>48Met Thr Asn Gly Lys Leu Ile Leu Leu Arg His Gly Gln Ser Glu Trp1 5 10 15Asn Ala Ser Asn Gln Phe Thr Gly Trp Val Asp Val Asn Leu Thr Glu
20 25 30Gln Gly Glu Ala Glu Ala Lys Gly Val Leu Pro Gly Val Val Tyr Thr
35 40 45Ser Leu Leu Arg Arg Ala Ile Arg Thr Ala Asn Ile Ala Leu Asn Ala
50 55 60Ala Asp Arg His Trp Ile Pro Val Ile Arg Asp Trp Arg Leu Asn Glu65 70 75 80Arg His Tyr Gly Ala Leu Gln Gly Leu Asp Lys Ala Ala Thr Lys Glu
85 90 95Lys Tyr Gly Asp Asp Gln Phe Met Glu Trp Arg Arg Ser Tyr Asp Thr
100 105 110Pro Pro Pro Glu Leu Ala Asp Asp Ala Glu Tyr Ser Gln Ala Asn Asp
115 120 125Pro Arg Tyr Ala Asp Leu Asp Val Val Pro Arg Thr Glu Cys Leu Lys
130 135 140Asp Val Val Val Arg Phe Val Pro Tyr Phe Glu Glu Glu Ile Leu Pro145 150 155 160Arg Ala Lys Lys Gly Glu Thr Val Leu Ile Ala Ala His Gly Asn Ser
165 170 175Leu Arg Ala Leu Val Lys His Leu Asp Gly Ile Ser Asp Ala Asp Ile
180 185 190Ala Glu Leu Asn Ile Pro Thr Gly Ile Pro Leu Val Tyr Glu Ile Ala
195 200 205Glu Asp Gly Ser Val Val Asn Pro Gly Gly Thr Tyr Leu Asp Pro Glu
210 215 220Ala Ala Ala Ala Gly Ala Ala Ala Val Ala Asn Gln Gly Asn Lys225 230 235<210>49<211>729<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(706)<223>RXA00381<400>49aaacctggtt tgtgtcttcc gctctcacgg acgatgattt ctccaagatc gagcaggcac 60tcaagcccgc cgcacgtgca gcagcagaag cgaaggcatc atg acg caa acc att 115
Met Thr Gln Thr Ile
1 5gtc cat cta gtt cgc cac ggc gaa gtc cac aac cca gag aaa atc ctg 163Val His Leu Val Arg His Gly Glu Val His Asn Pro Glu Lys Ile Leu
10 15 20tac gga cgc atg ccc gga tac agg ttg tct tcc cgt gga cgc agc caa 211Tyr Gly Arg Met Pro Gly Tyr Arg Leu Ser Ser Arg Gly Arg Ser Gln
25 30 35gcc gcc cgc act gca gct tct ttt gaa ggc cac gat gtc acc tac att 259Ala Ala Arg Thr Ala Ala Ser Phe Glu Gly His Asp Val Thr Tyr Ile
40 45 50gcg gcc tcc cca ttg cag cgt gtg cag gaa acc tcc gaa ccg ttc atc 307Ala Ala Ser Pro Leu Gln Arg Val Gln Glu Thr Ser Glu Pro Phe Ile
55 60 65aag gtc aca ggc cta gaa ctg atc acc gac gag gat ctt ctg gaa gca 355Lys Val Thr Gly Leu Glu Leu Ile Thr Asp Glu Asp Leu Leu Glu Ala70 75 80 85ggc aac cgt ttc gaa ggc ctg cgc acc aaa ggt tgg cgt tcc cag ttg 403Gly Asn Arg Phe Glu Gly Leu Arg Thr Lys Gly Trp Arg Ser Gln Leu
90 95 100tgg aac ccc gtg cgt tgg cct ttg atg tac aac ccc acg ctt ccc agc 451Trp Asn Pro Val Arg Trp Pro Leu Met Tyr Asn Pro Thr Leu Pro Ser
105 110 115tgg ggc gaa cac tac acc gac att ttg gaa aga atg atg gcg gct gtg 499Trp Gly Glu His Tyr Thr Asp Ile Leu Glu Arg Met Met Ala Ala Val
120 125 130gaa cga gct cgg gtg gca gcg gaa gga cac gaa gca atc ctg gtg acc 547Glu Arg Ala Arg Val Ala Ala Glu Gly His Glu Ala Ile Leu Val Thr
135 140 145cac cag ttg ccg atc gtg tgc gtg caa cgc cac gcc cgc gga caa agc 595His Gln Leu Pro Ile Val Cys Val Gln Arg His Ala Arg Gly Gln Ser150 155 160 165ctg tcc cat aac cca gcg acc agg caa tgc gac ctc gcc tca gtg aca 643Leu Ser His Asn Pro Ala Thr Arg Gln Cys Asp Leu Ala Ser Val Thr
170 175 180tcc ttg gtg ttc caa gac gat caa att gtc ggc gtg cat tac aac gaa 691Ser Leu Val Phe Gln Asp Asp Gln Ile Val Gly Val His Tyr Asn Glu
185 190 195cca gct cag ggg att tgatcactcg tgcgtttgac caa 729Pro Ala Gln Glu Ile
200<210>50<211>202<212>PRT<213>谷氨酸棒杆菌<400> 50Met Thr Gln Thr Ile Val His Leu Val Arg His Gly Glu Val His Asn1 5 10 15Pro Glu Lys Ile Leu Tyr Gly Arg Met Pro Gly Tyr Arg Leu Ser Ser
20 25 30Arg Gly Arg Ser Gln Ala Ala Arg Thr Ala Ala Ser Phe Glu Gly His
35 40 45Asp Val Thr Tyr Ile Ala Ala Ser Pro Leu Gln Arg Val Gln Glu Thr
50 55 60Ser Glu Pro Phe Ile Lys Val Thr Gly Leu Glu Leu Ile Thr Asp Glu65 70 75 80Asp Leu Leu Glu Ala Gly Asn Arg Phe Glu Gly Leu Arg Thr Lys Gly
85 90 95Trp Arg Ser Gln Leu Trp Asn Pro Val Arg Trp Pro Leu Met Tyr Asn
100 105 110Pro Thr Leu Pro Ser Trp Gly Glu His Tyr Thr Asp Ile Leu Glu Arg
115 120 125Met Met Ala Ala Val Glu Arg Ala Arg Val Ala Ala Glu Gly His Glu
130 135 140Ala Ile Leu Val Thr His Gln Leu Pro Ile Val Cys Val Gln Arg His145 150 155 160Ala Arg Gly Gln Ser Leu Ser His Asn Pro Ala Thr Arg Gln Cys Asp
165 170 175Leu Ala Ser Val Thr Ser Leu Val Phe Gln Asp Asp Gln Ile Val Gly
180 185 190Val His Tyr Asn Glu Pro Ala Gln Glu Ile
195 200<210>51<211>822<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(799)<223>RXA02122<400> 51ggccttcagc tcaccgacag tttgtacatt tggtggaaga ctcacacccc acaccctaga 60ccttttttta agtgggcggt caggaatttt tcgcacaggt atg ctg cat gtc atg 115
Met Leu His Val Met
1 5aag ccg ggt tca cac gca gct gcc gaa aag act caa tcc act gtg gtt 163Lys Pro Gly Ser His Ala Ala Ala Glu Lys Thr Gln Ser Thr Val Val
10 15 20tta ctc att cgg cat ggg caa acc cca aca act ggt cag gtt ctg cct 211Leu Leu Ile Arg His Gly Gln Thr Pro Thr Thr Gly Gln Val Leu Pro
25 30 35ggt cag acg ccg ggt tta cac ctg tct gat aag ggt gaa gag cag gcg 259Gly Gln Thr Pro Gly Leu His Leu Ser Asp Lys Gly Glu Glu Gln Ala
40 45 50cgg gag gtg gca cag cgt ctg gcg gag gtg ccg att acc gct gtg tat 307Arg Glu Val Ala Gln Arg Leu Ala Glu Val Pro Ile Thr Ala Val Tyr
55 60 65tca tcg ccg atg gag cgt gcg cag gaa aca gca gca ccg acg gtc agc 355Ser Ser Pro Met Glu Arg Ala Gln Glu Thr Ala Ala Pro Thr Val Ser70 75 80 85gct cat ggc ctc gag ttg acg gtg gaa cct ggg ctt att gaa tgc gat 403Ala His Gly Leu Glu Leu Thr Val Glu Pro Gly Leu Ile Glu Cys Asp
90 95 100ttc ggc gag tgg acg ggc cgg aaa cta act gag ctc aat gcc cta gag 451Phe Gly Glu Trp Thr Gly Arg Lys Leu Thr Glu Leu Asn Ala Leu Glu
105 110 115gag tgg aaa gcg gtg cag aag aca ccg tct acc ttc agg ttt cca ggt 499Glu Trp Lys Ala Val Gln Lys Thr Pro Ser Thr Phe Arg Phe Pro Gly
120 125 130ggt gag agt ttc gtg gaa atg cag gat cgg atg gtg gag gct atc ggc 547Gly Glu Ser Phe Val Glu Met Gln Asp Arg Met Val Glu Ala Ile Gly
135 140 145aac att gcg cag cag cat ccg gga gaa atc gtt gct gcg ttt agt cat 595Asn Ile Ala Gln Gln His Pro Gly Glu Ile Val Ala Ala Phe Ser His150 155 160 165gcc gac acg atc aag gct gcg gtg gct cat ttt gta ggc act cca ctg 643Ala Asp Thr Ile Lys Ala Ala Val Ala His Phe Val Gly Thr Pro Leu
170 175 180gat tct ttt cag cgc att ttc atc gac acg gcg tca att tcc gca gtg 691Asp Ser Phe Gln Arg Ile Phe Ile Asp Thr Ala Ser Ile Ser Ala Val
185 190 195gaa ttt acc ggg aaa tct tca ggc gtc tcc tcc cat atg ctg ctg aca 739Glu Phe Thr Gly Lys Ser Ser Gly Val Ser Ser His Met Leu Leu Thr
200 205 210aat tcc aga aca gga tcg ttg gga tac ctt cga gac aaa ctt ccg aaa 787Asn Ser Arg Thr Gly Ser Leu Gly Tyr Leu Arg Asp Lys Leu Pro Lys
215 220 225gct ccg caa cca tgatcacctc accatttgag cgc 822Ala Pro Gln Pro230<210>52<211>233<212>PRT<213>谷氨酸棒杆菌<400> 52Met Leu His Val Met Lys Pro Gly Ser His Ala Ala Ala Glu Lys Thr1 5 10 15Gln Ser Thr Val Val Leu Leu Ile Arg His Gly Gln Thr Pro Thr Thr
20 25 30Gly Gln Val Leu Pro Gly Gln Thr Pro Gly Leu His Leu Ser Asp Lys
35 40 45Gly Glu Glu Gln Ala Arg Glu Val Ala Gln Arg Leu Ala Glu Val Pro
50 55 60Ile Thr Ala Val Tyr Ser Ser Pro Met Glu Arg Ala Gln Glu Thr Ala65 70 75 80Ala Pro Thr Val Ser Ala His Gly Leu Glu Leu Thr Val Glu Pro Gly
85 90 95Leu Ile Glu Cys Asp Phe Gly Glu Trp Thr Gly Arg Lys Leu Thr Glu
100 105 110Leu Asn Ala Leu Glu Glu Trp Lys Ala Val Gln Lys Thr Pro Ser Thr
115 120 125Phe Arg Phe Pro Gly Gly Glu Ser Phe Val Glu Met Gln Asp Arg Met
130 135 140Val Glu Ala Ile Gly Asn Ile Ala Gln Gln His Pro Gly Glu Ile Val145 150 155 160Ala Ala Phe Ser His Ala Asp Thr Ile Lys Ala Ala Val Ala His Phe
165 170 175Val Gly Thr Pro Leu Asp Ser Phe Gln Arg Ile Phe Ile Asp Thr Ala
180 185 190Ser Ile Ser Ala Val Glu Phe Thr Gly Lys Ser Ser Gly Val Ser Ser
195 200 205His Met Leu Leu Thr Asn Ser Arg Thr Gly Ser Leu Gly Tyr Leu Arg
210 215 220Asp Lys Leu Pro Lys Ala Pro Gln Pro225 230<210>53<211>1161<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1138)<223>RXA00206<400>53ttaaataaga tggtcagaga cagttttttg gcctgtcaac ccctgtgatt ctcttatttt 60tgggtgattg ttccggcgcg ggtgttgtga tgggtttaat atg gaa gac atg cga 115
Met Glu Asp Met Arg
1 5att gct act ctc acg tca ggc ggc gac tgc ccc gga cta aac gcc gtc 163Ile Ala Thr Leu Thr Ser Gly Gly Asp Cys Pro Gly Leu Asn Ala Val
10 15 20atc cga gga atc gtc cgc aca gcc agc aat gaa ttt ggc tcc acc gtc 211Ile Arg Gly Ile Val Arg Thr Ala Ser Asn Glu Phe Gly Ser Thr Val
25 30 35gtt ggt tat caa gac ggt tgg gaa gga ctg tta ggc gat cgt cgc gta 259Val Gly Tyr Gln Asp Gly Trp Glu Gly Leu Leu Gly Asp Arg Arg Val
40 45 50cag ctg tat gac gat gaa gat att gac cga atc ctc ctt cga ggc ggc 307Gln Leu Tyr Asp Asp Glu Asp Ile Asp Arg Ile Leu Leu Arg Gly Gly
55 60 65acc att ttg ggc act ggt cgc ctc cat ccg gac aag ttt aag gcc gga 355Thr Ile Leu Gly Thr Gly Arg Leu His Pro Asp Lys Phe Lys Ala Gly70 75 80 85att gat cag att aag gcc aac tta gaa gac gcc ggc atc gat gcc ctt 403Ile Asp Gln Ile Lys Ala Asn Leu Glu Asp Ala Gly Ile Asp Ala Leu
90 95 100atc cca atc ggt ggc gaa gga acc ctg aag ggt gcc aag tgg ctg tct 451Ile Pro Ile Gly Gly Glu Gly Thr Leu Lys Gly Ala Lys Trp Leu Ser
105 110 115gat aac ggt atc cct gtt gtc ggt gtc cca aag acc att gac aat gac 499Asp Asn Gly Ile Pro Val Val Gly Val Pro Lys Thr Ile Asp Asn Asp
120 125 130gtg aat ggc act gac ttc acc ttc ggt ttc gat act gct gtg gca gtg 547Val Asn Gly Thr Asp Phe Thr Phe Gly Phe Asp Thr Ala Val Ala Val
135 140 145gct acc gac gct gtt gac cgc ctg cac acc acc gct gaa tct cac aac 595Ala Thr Asp Ala Val Asp Arg Leu His Thr Thr Ala Glu Ser His Asn150 155 160 165cgt gtg atg atc gtg gag gtc atg ggc cgc cac gtg ggt tgg att gct 643Arg Val Met Ile Val Glu Val Met Gly Arg His Val Gly Trp Ile Ala
170 175 180ctg cac gca ggt atg gcc ggc ggt gct cac tac acc gtt att cca gaa 691Leu His Ala Gly Met Ala Gly Gly Ala His Tyr Thr Val Ile Pro Glu
185 190 195gta cct ttc gat att gca gag atc tgc aag gcg atg gaa cgt cgc ttc 739Val Pro Phe Asp Ile Ala Glu Ile Cys Lys Ala Met Glu Arg Arg Phe
200 205 210cag atg ggc gag aag tac ggc att atc gtc gtt gcg gaa ggt gcg ttg 787Gln Met Gly Glu Lys Tyr Gly Ile Ile Val Val Ala Glu Gly Ala Leu
215 220 225cca cgc gaa ggc acc atg gag ctt cgt gaa ggc cac att gac cag ttc 835Pro Arg Glu Gly Thr Met Glu Leu Arg Glu Gly His Ile Asp Gln Phe230 235 240 245ggt cac aag acc ttc acg gga att gga cag cag atc gct gat gag atc 883Gly His Lys Thr Phe Thr Gly Ile Gly Gln Gln Ile Ala Asp Glu Ile
250 255 260cac gtg cgc ctc ggc cac gat gtt cgt acg acc gtt ctt ggc cac att 931His Val Arg Leu Gly His Asp Val Arg Thr Thr Val Leu Gly His Ile
265 270 275caa cgt ggt gga acc cca act gct ttc gac cgt gtt ctg gcc act cgt 979Gln Arg Gly Gly Thr Pro Thr Ala Phe Asp Arg Val Leu Ala Thr Arg
280 285 290tat ggt gtt cgt gca gct cgt gcg tgc cat gag gga agc ttt gac aag 1027Tyr Gly Val Arg Ala Ala Arg Ala Cys His Glu Gly Ser Phe Asp Lys
295 300 305gtt gtt gct ttg aag ggt gag agc att gag atg atc acc ttt gaa gaa 1075Val Val Ala Leu Lys Gly Glu Ser Ile Glu Met Ile Thr Phe Glu Glu310 315 320 325gca gtc gga acc ttg aag gaa gtt cca ttc gaa cgc tgg gtt act gcc 1123Ala Val Gly Thr Leu Lys Glu Val Pro Phe Glu Arg Trp Val Thr Ala
330 335 340cag gca atg ttt gga tagtttttcg ggcttttatc aac 1161Gln Ala Met Phe Gly
345<210>54<211>346<212>PRT<213>谷氨酸棒杆菌<400>54Met Glu Asp Met Arg Ile Ala Thr Leu Thr Ser Gly Gly Asp Cys Pro1 5 10 15Gly Leu Asn Ala Val Ile Arg Gly Ile Val Arg Thr Ala Ser Asn Glu
20 25 30Phe Gly Ser Thr Val Val Gly Tyr Gln Asp Gly Trp Glu Gly Leu Leu
35 40 45Gly Asp Arg Arg Val Gln Leu Tyr Asp Asp Glu Asp Ile Asp Arg Ile
50 55 60Leu Leu Arg Gly Gly Thr Ile Leu Gly Thr Gly Arg Leu His Pro Asp65 70 75 80Lys Phe Lys Ala Gly Ile Asp Gln Ile Lys Ala Asn Leu Glu Asp Ala
85 90 95Gly Ile Asp Ala Leu Ile Pro Ile Gly Gly Glu Gly Thr Leu Lys Gly
100 105 110Ala Lys Trp Leu Ser Asp Asn Gly Ile Pro Val Val Gly Val Pro Lys
115 120 125Thr Ile Asp Asn Asp Val Asn Gly Thr Asp Phe Thr Phe Gly Phe Asp
130 135 140Thr Ala Val Ala Val Ala Thr Asp Ala Val Asp Arg Leu His Thr Thr145 150 155 160Ala Glu Ser His Asn Arg Val Met Ile Val Glu Val Met Gly Arg His
165 170 175Val Gly Trp Ile Ala Leu His Ala Gly Met Ala Gly Gly Ala His Tyr
180 185 190Thr Val Ile Pro Glu Val Pro Phe Asp Ile Ala Glu Ile Cys Lys Ala
195 200 205Met Glu Arg Arg Phe Gln Met Gly Glu Lys Tyr Gly Ile Ile Val Val
210 215 220Ala Glu Gly Ala Leu Pro Arg Glu Gly Thr Met Glu Leu Arg Glu Gly225 230 235 240His Ile Asp Gln Phe Gly His Lys Thr Phe Thr Gly Ile Gly Gln Gln
245 250 255Ile Ala Asp Glu Ile His Val Arg Leu Gly His Asp Val Arg Thr Thr
260 265 270Val Leu Gly His Ile Gln Arg Gly Gly Thr Pro Thr Ala Phe Asp Arg
275 280 285Val Leu Ala Thr Arg Tyr Gly Val Arg Ala Ala Arg Ala Cys His Glu
290 295 300Gly Ser Phe Asp Lys Val Val Ala Leu Lys Gly Glu Ser Ile Glu Met305 310 315 320Ile Thr Phe Glu Glu Ala Val Gly Thr Leu Lys Glu Val Pro Phe Glu
325 330 335Arg Trp Val Thr Ala Gln Ala Met Phe Gly
340 345<210>55<211>1083<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1060)<223>RXA01243<400>55gcgcaatcag cgatatcgat gtggtggtca ccgatgcggg tgcaccagca agtttcgttg 60agcagttgcg agaacgcgat gtagaagttg tgattgcaga atg att ctt aca gtc 115
Met Ile Leu Thr Val
1 5act gca agt ccg tat ctg ttg agc acc aat gag ctt gac ggc acc atc 163Thr Ala Ser Pro Tyr Leu Leu Ser Thr Asn Glu Leu Asp Gly Thr Ile
10 15 20gaa att ggc gaa gca aac aaa atc cgg cag gtt tcc act gtt gcc ggt 211Glu Ile Gly Glu Ala Asn Lys Ile Arg Gln Val Ser Thr Val Ala Gly
25 30 35ggt ttt ggc acc ggt gtg gct gcc acc ttg ttt tat ggc ggc aat gaa 259Gly Phe Gly Thr Gly Val Ala Ala Thr Leu Phe Tyr Gly Gly Asn Glu
40 45 50act ttt gca gtt ttt ccc gct cca gaa atc tct cat tac atg cgc ctg 307Thr Phe Ala Val Phe Pro Ala Pro Glu Ile Ser His Tyr Met Arg Leu
55 60 65gtg acg ttt gct ggg ttg cct cat gaa att att ccg gtg gca ggt ccc 355Val Thr Phe Ala Gly Leu Pro His Glu Ile Ile Pro Val Ala Gly Pro70 75 80 85atc ccc atg cat ttg acc atg cgt gat gca gag ggc aat gag act aag 403Ile Pro Met His Leu Thr Met Arg Asp Ala Glu Gly Asn Glu Thr Lys
90 95 100ttc aaa gac tcc ccc atg cct ttg gat gtg tcc cag ttg gca att ctt 451Phe Lys Asp Ser Pro Met Pro Leu Asp Val Ser Gln Leu Ala Ile Leu
105 110 115cgt gat cta gtg gtg cgt cga gcc gaa gat gcc gcg tgg gtg ttg ttg 499Arg Asp Leu Val Val Arg Arg Ala Glu Asp Ala Ala Trp Val Leu Leu
120 125 130ggt ggc aat ttg ccg tct atc gcg cct gct gcg tgg ttt gtg gat gtg 547Gly Gly Asn Leu Pro Ser Ile Ala Pro Ala Ala Trp Phe Val Asp Val
135 140 145gtg aga tca ctt cgc ttg tac cac cct cat gtg aag gta gct atc gca 595Val Arg Ser Leu Arg Leu Tyr His Pro His Val Lys Val Ala Ile Ala150 155 160 165gca act ggt gct gcg ttg cgt gcg gtt att cga cag ctt gca gct acg 643Ala Thr Gly Ala Ala Leu Arg Ala Val Ile Arg Gln Leu Ala Ala Thr
170 175 180tcc ccg gat gcg ctg att gtg gct gcg gaa gaa atc gaa att gcc act 691Ser Pro Asp Ala Leu Ile Val Ala Ala Glu Glu Ile Glu Ile Ala Thr
185 190 195gga tta gaa ccc aaa acc ttg aga ggt cca tgg gta gag gga gat ctc 739Gly Leu Glu Pro Lys Thr Leu Arg Gly Pro Trp Val Glu Gly Asp Leu
200 205 210tcc ccg act gtg gcg gca gcg cgc gct tta att gat agc ggt gtc acc 787Ser Pro Thr Val Ala Ala Ala Arg Ala Leu Ile Asp Ser Gly Val Thr
215 220 225gag gtg ttg gtt acc aac aag cgg acg gaa tct ttg tat gtt tcc gag 835Glu Val Leu Val Thr Asn Lys Arg Thr Glu Ser Leu Tyr Val Ser Glu230 235 240 245tct gaa tca ctg tta gcc agc tac gac agc acc cct ggt aag cag ggc 883Ser Glu Ser Leu Leu Ala Ser Tyr Asp Ser Thr Pro Gly Lys Gln Gly
250 255 260gtg aat tgg cgg gaa tct ttt act gca gga ttc ttg gca gca tcc aat 931Val Asn Trp Arg Glu Ser Phe Thr Ala Gly Phe Leu Ala Ala Ser Asn
265 270 275gat gga aaa tcc act gag gac agc gtg atc aac gcg gtt gct tac gcc 979Asp Gly Lys Ser Thr Glu Asp Ser Val Ile Asn Ala Val Ala Tyr Ala
280 285 290aac gct gaa ggc agt gag tgg gac aac tac att ccc aca ccc gat aag 1027Asn Ala Glu Gly Ser Glu Trp Asp Asn Tyr Ile Pro Thr Pro Asp Lys
295 300 305ctt cgg gcg gag cac gtg gtc atc aaa tcg ctt tagaccacgc aaaaagcctc 1080Leu Arg Ala Glu His Val Val Ile Lys Ser Leu310 315 320aaa 1083<210>56<211>320<212>PRT<213>谷氨酸棒杆菌<400>56Met Ile Leu Thr Val Thr Ala Ser Pro Tyr Leu Leu Ser Thr Asn Glu1 5 10 15Leu Asp Gly Thr Ile Glu Ile Gly Glu Ala Asn Lys Ile Arg Gln Val
20 25 30Ser Thr Val Ala Gly Gly Phe Gly Thr Gly Val Ala Ala Thr Leu Phe
35 40 45Tyr Gly Gly Asn Glu Thr Phe Ala Val Phe Pro Ala Pro Glu Ile Ser
50 55 60His Tyr Met Arg Leu Val Thr Phe Ala Gly Leu Pro His Glu Ile Ile65 70 75 80Pro Val Ala Gly Pro Ile Pro Met His Leu Thr Met Arg Asp Ala Glu
85 90 95Gly Asn Glu Thr Lys Phe Lys Asp Ser Pro Met Pro Leu Asp Val Ser
100 105 110Gln Leu Ala Ile Leu Arg Asp Leu Val Val Arg Arg Ala Glu Asp Ala
115 120 125Ala Trp Val Leu Leu Gly Gly Asn Leu Pro Ser Ile Ala Pro Ala Ala
130 135 140Trp Phe Val Asp Val Val Arg Ser Leu Arg Leu Tyr His Pro His Val145 150 155 160Lys Val Ala Ile Ala Ala Thr Gly Ala Ala Leu Arg Ala Val Ile Arg
165 170 175Gln Leu Ala Ala Thr Ser Pro Asp Ala Leu Ile Val Ala Ala Glu Glu
180 185 190Ile Glu Ile Ala Thr Gly Leu Glu Pro Lys Thr Leu Arg Gly Pro Trp
195 200 205Val Glu Gly Asp Leu Ser Pro Thr Val Ala Ala Ala Arg Ala Leu Ile
210 215 220Asp Ser Gly Val Thr Glu Val Leu Val Thr Asn Lys Arg Thr Glu Ser225 230 235 240Leu Tyr Val Ser Glu Ser Glu Ser Leu Leu Ala Ser Tyr Asp Ser Thr
245 250 255Pro Gly Lys Gln Gly Val Asn Trp Arg Glu Ser Phe Thr Ala Gly Phe
260 265 270Leu Ala Ala Ser Asn Asp Gly Lys Ser Thr Glu Asp Ser Val Ile Asn
275 280 285Ala Val Ala Tyr Ala Asn Ala Glu Gly Ser Glu Trp Asp Asn Tyr Ile
290 295 300Pro Thr Pro Asp Lys Leu Arg Ala Glu His Val Val Ile Lys Ser Leu305 310 315 320<210>57<211>1113<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1090)<223>RXA01882<400>57accctttttc caccttggct gattttgatg cccttgttac cgatgaccac acgctagatt 60ttccagtttt gcccgaccac aactttcagg tggtaacccc atg atc atc aca ttc 115
Met Ile Ile Thr Phe
1 5acc cca aac ccg agt att gat tcc acg ctg tcg ctc ggc gaa gag ctc 163Thr Pro Asn Pro Ser Ile Asp Ser Thr Leu Ser Leu Gly Glu Glu Leu
10 15 20tcc cgt gga tcc gtc caa cga ctt gat tcc gtc acc gct gtc gca ggt 211Ser Arg Gly Ser Val Gln Arg Leu Asp Ser Val Thr Ala Val Ala Gly
25 30 35ggt aaa ggc atc aat gtc gcc cac gct gtc ttg ctt gcg ggc ttt gaa 259Gly Lys Gly Ile Asn Val Ala His Ala Val Leu Leu Ala Gly Phe Glu
40 45 50acc ttg gct gtg ttc cca gcc ggc aag ctc gac ccc ttc gtc cca ctg 307Thr Leu Ala Val Phe Pro Ala Gly Lys Leu Asp Pro Phe Val Pro Leu
55 60 65gtc cgc gac atc ggc ttg ccc gtg gaa act gtt gtg atc aac aag aac 355Val Arg Asp Ile Gly Leu Pro Val Glu Thr Val Val Ile Asn Lys Asn70 75 80 85gtc cgc acc aac acc aca gtc acc gaa ccg gac ggc acc acc acc aag 403Val Arg Thr Asn Thr Thr Val Thr Glu Pro Asp Gly Thr Thr Thr Lys
90 95 100ctc aac ggc ccc ggc gcg ccg ctc agc gag cag aag ctc cgt agc ttg 451Leu Asn Gly Pro Gly Ala Pro Leu Ser Glu Gln Lys Leu Arg Ser Leu
105 110 115gaa aag gtg ctt atc gac gcg ctc cgc ccc gaa gtc acc tgg gtt gtc 499Glu Lys Val Leu Ile Asp Ala Leu Arg Pro Glu Val Thr Trp Val Val
120 125 130ctg gcg ggc tcg ctg cca cca ggg gca cca gtt gac tgg tac gcg cgt 547Leu Ala Gly Ser Leu Pro Pro Gly Ala Pro Val Asp Trp Tyr Ala Arg
135 140 145ctc acc gcg ttg atc cat tca gca cgc cct gac gtt cgc gtg gct gtc 595Leu Thr Ala Leu Ile His Ser Ala Arg Pro Asp Val Arg Val Ala Val150 155 160 165gat acc tca gac aag cca ctg atg gcg ttg ggc gag agc ttg gat aca 643Asp Thr Ser Asp Lys Pro Leu Met Ala Leu Gly Glu Ser Leu Asp Thr
170 175 180cct ggc gct gct ccg aac ctg att aag cca aat ggt ctg gaa ctg ggc 691Pro Gly Ala Ala Pro Asn Leu Ile Lys Pro Asn Gly Leu Glu Leu Gly
185 190 195cag ctg gct aac act gat ggt gaa gag ctg gag gcg cgt gct gcg caa 739Gln Leu Ala Asn Thr Asp Gly Glu Glu Leu Glu Ala Arg Ala Ala Gln
200 205 210ggc gat tac gac gcc atc atc gca gct gcg gac gta ctg gtt aac cgt 787Gly Asp Tyr Asp Ala Ile Ile Ala Ala Ala Asp Val Leu Val Asn Arg
215 220 225ggc atc gaa cag gtg ctt gtc acc ttg ggt gcc gca gga gcg gtg ttg 835Gly Ile Glu Gln Val Leu Val Thr Leu Gly Ala Ala Gly Ala Val Leu230 235 240 245gtc aac gca gaa ggt gcg tgg act gct act tct cca aag att gat gtt 883Val Asn Ala Glu Gly Ala Trp Thr Ala Thr Ser Pro Lys Ile Asp Val
250 255 260gta tcc acc gtt gga gct gga gac tgt gct ctt gca ggt ttt gtt atg 931Val Ser Thr Val Gly Ala Gly Asp Cys Ala Leu Ala Gly Phe Val Met
265 270 275gca cgt tcc cag aag aaa aca ctg gag gaa tct ctg ctg aat gcc gtg 979Ala Arg Ser Gln Lys Lys Thr Leu Glu Glu Ser Leu Leu Asn Ala Val
280 285 290tct tac ggc tcg act gcg gcg tct ctt cct ggc act acc att cct cgt 1027Ser Tyr Gly Ser Thr Ala Ala Ser Leu Pro Gly Thr Thr Ile Pro Arg
295 300 305cct gac caa ctc gcc aca gct ggt gca acg gtc acc caa gtc aaa gga 1075Pro Asp Gln Leu Ala Thr Ala Gly Ala Thr Val Thr Gln Val Lys Gly310 315 320 325ttg aaa gaa tca gca tgaatagcgt aaataattcc tcg 1113Leu Lys Glu Ser Ala
330<210>58<211>330<212>PRT<213>谷氨酸棒杆菌<400>58Met Ile Ile Thr Phe Thr Pro Asn Pro Ser Ile Asp Ser Thr Leu Ser1 5 10 15Leu Gly Glu Glu Leu Ser Arg Gly Ser Val Gln Arg Leu Asp Ser Val
20 25 30Thr Ala Val Ala Gly Gly Lys Gly Ile Asn Val Ala His Ala Val Leu
35 40 45Leu Ala Gly Phe Glu Thr Leu Ala Val Phe Pro Ala Gly Lys Leu Asp
50 55 60Pro Phe Val Pro Leu Val Arg Asp Ile Gly Leu Pro Val Glu Thr Val65 70 75 80Val Ile Asn Lys Asn Val Arg Thr Asn Thr Thr Val Thr Glu Pro Asp
85 90 95Gly Thr Thr Thr Lys Leu Asn Gly Pro Gly Ala Pro Leu Ser Glu Gln
100 105 110Lys Leu Arg Ser Leu Glu Lys Val Leu Ile Asp Ala Leu Arg Pro Glu
115 120 125Val Thr Trp Val Val Leu Ala Gly Ser Leu Pro Pro Gly Ala Pro Val
130 135 140Asp Trp Tyr Ala Arg Leu Thr Ala Leu Ile His Ser Ala Arg Pro Asp145 150 155 160Val Arg Val Ala Val Asp Thr Ser Asp Lys Pro Leu Met Ala Leu Gly
165 170 175Glu Ser Leu Asp Thr Pro Gly Ala Ala Pro Asn Leu Ile Lys Pro Asn
180 185 190Gly Leu Glu Leu Gly Gln Leu Ala Asn Thr Asp Gly Glu Glu Leu Glu
195 200 205Ala Arg Ala Ala Gln Gly Asp Tyr Asp Ala Ile Ile Ala Ala Ala Asp
210 215 220Val Leu Val Asn Arg Gly Ile Glu Gln Val Leu Val Thr Leu Gly Ala225 230 235 240Ala Gly Ala Val Leu Val Asn Ala Glu Gly Ala Trp Thr Ala Thr Ser
245 250 255Pro Lys Ile Asp Val Val Ser Thr Val Gly Ala Gly Asp Cys Ala Leu
260 265 270Ala Gly Phe Val Met Ala Arg Ser Gln Lys Lys Thr Leu Glu Glu Ser
275 280 285Leu Leu Asn Ala Val Ser Tyr Gly Ser Thr Ala Ala Ser Leu Pro Gly
290 295 300Thr Thr Ile Pro Arg Pro Asp Gln Leu Ala Thr Ala Gly Ala Thr Val305 310 315 320Thr Gln Val Lys Gly Leu Lys Glu Ser Ala
325 330<210>59<211>1155<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1132)<223>RXA01702<400>59attgtgaatg tgggattatc ggttcgcgct tcaccatgtt tctgcatgat gaaattacat 60acatagttca gtgacagtca ccttttggag gagacacctt atg cct atc gca act 115
Met Pro Ile Ala Thr
1 5ccc gag gtc tat aac gag atg ctc gat cgt gct aag gaa ggc gga ttc 163Pro Glu Val Tyr Asn Glu Met Leu Asp Arg Ala Lys Glu Gly Gly Phe
10 15 20gcc ttc cca gcc atc aac tgc acc tcc tcg gaa acc atc aac gca gct 211Ala Phe Pro Ala Ile Asn Cys Thr Ser Ser Glu Thr Ile Asn Ala Ala
25 30 35ctc aag ggc ttc gca gag gct gaa tct gac gga atc atc cag ttc tcc 259Leu Lys Gly Phe Ala Glu Ala Glu Ser Asp Gly Ile Ile Gln Phe Ser
40 45 50acc ggt ggt gca gag ttc ggt tcc ggc ctg gca gta aag aac aag gtc 307Thr Gly Gly Ala Glu Phe Gly Ser Gly Leu Ala Val Lys Asn Lys Val
55 60 65aag ggc gca gtt gcg ctt gca gcc ttc gcc cac gag gca gca aag agc 355Lys Gly Ala Val Ala Leu Ala Ala Phe Ala His Glu Ala Ala Lys Ser70 75 80 85tac ggc atc aac gtt gct ctg cac act gac cac tgc cag aag gaa gtc 403Tyr Gly Ile Asn Val Ala Leu His Thr Asp His Cys Gln Lys Glu Val
90 95 100ctg gac gag tac gtc cgc cca ctg ctg gct atc tcc cag gag cgc gtc 451Leu Asp Glu Tyr Val Arg Pro Leu Leu Ala Ile Ser Gln Glu Arg Val
105 110 115gac cgc ggc gag ctt cca ctg ttc cag tcc cac atg tgg gat ggt tcc 499Asp Arg Gly Glu Leu Pro Leu Phe Gln Ser His Met Trp Asp Gly Ser
120 125 130gct gtc cca atc gac gag aac ctc gaa atc gca cag gag ctg ctg gct 547Ala Val Pro Ile Asp Glu Asn Leu Glu Ile Ala Gln Glu Leu Leu Ala
135 140 145aag gcc aag gca gcg aac atc atc ttg gaa gtt gag atc ggt gtt gtc 595Lys Ala Lys Ala Ala Asn Ile Ile Leu Glu Val Glu Ile Gly Val Val150 155 160 165ggt ggc gaa gaa gac ggc gtt gag gct aag gct ggc gca aac ctc tac 643Gly Gly Glu Glu Asp Gly Val Glu Ala Lys Ala Gly Ala Asn Leu Tyr
170 175 180acc tcc cca gaa gac ttt gag aag acc atc gat gca atc ggc acc ggt 691Thr Ser Pro Glu Asp Phe Glu Lys Thr Ile Asp Ala Ile Gly Thr Gly
185 190 195gag aag ggc cgc tac ctg cta gca gct acc ttc ggt aac gtc cac ggc 739Glu Lys Gly Arg Tyr Leu Leu Ala Ala Thr Phe Gly Asn Val His Gly
200 205 210gtt tac aag cca ggc aac gtc aag ctg cgc cca gag gtc ctc ctt gag 787Val Tyr Lys Pro Gly Asn Val Lys Leu Arg Pro Glu Val Leu Leu Glu
215 220 225ggc cag cag gtt gca cgc aag aag ctt gga ctt gca gac gac gca ctt 835Gly Gln Gln Val Ala Arg Lys Lys Leu Gly Leu Ala Asp Asp Ala Leu230 235 240 245cca ttc gac ttc gtc ttc cac ggt ggc tca ggc tcc gag aag gaa aag 883Pro Phe Asp Phe Val Phe His Gly Gly Ser Gly Ser Glu Lys Glu Lys
250 255 260atc gaa gag gcg ctg acc tac ggc gtc atc aag atg aac gtt gat act 931Ile Glu Glu Ala Leu Thr Tyr Gly Val Ile Lys Met Asn Val Asp Thr
265 270 275gac acc cag tac gca ttc acc cgc cca atc gtc tcc cac atg ttt gag 979Asp Thr Gln Tyr Ala Phe Thr Arg Pro Ile Val Ser His Met Phe Glu
280 285 290aac tac aac ggc gtt ctc aag atc gac ggc gag gtc gga aac aag aag 1027Asn Tyr Asn Gly Val Leu Lys Ile Asp Gly Glu Val Gly Asn Lys Lys
295 300 305gct tac gac cca cgc tct tac atg aag aag gct gag cag agc atg tct 1075Ala Tyr Asp Pro Arg Ser Tyr Met Lys Lys Ala Glu Gln Ser Met Ser310 315 320 325gag cgc att atc gag tct tgc cag gac ctc aag tct gtt gga aag acc 1123Glu Arg Ile Ile Glu Ser Cys Gln Asp Leu Lys Ser Val Gly Lys Thr
330 335 340acc tct aag taatctcagc agttaaaaag ggc 1155Thr Ser Lys<210>60<211>344<212>PRT<213>谷氨酸棒杆菌<400>60Met Pro Ile Ala Thr Pro Glu Val Tyr Asn Glu Met Leu Asp Arg Ala1 5 10 15Lys Glu Gly Gly Phe Ala Phe Pro Ala Ile Asn Cys Thr Ser Ser Glu
20 25 30Thr Ile Asn Ala Ala Leu Lys Gly Phe Ala Glu Ala Glu Ser Asp Gly
35 40 45Ile Ile Gln Phe Ser Thr Gly Gly Ala Glu Phe Gly Ser Gly Leu Ala
50 55 60Val Lys Asn Lys Val Lys Gly Ala Val Ala Leu Ala Ala Phe Ala His65 70 75 80Glu Ala Ala Lys Ser Tyr Gly Ile Asn Val Ala Leu His Thr Asp His
85 90 95Cys Gln Lys Glu Val Leu Asp Glu Tyr Val Arg Pro Leu Leu Ala Ile
100 105 110Ser Gln Glu Arg Val Asp Arg Gly Glu Leu Pro Leu Phe Gln Ser His
115 120 125Met Trp Asp Gly Ser Ala Val Pro Ile Asp Glu Asn Leu Glu Ile Ala
130 135 140Gln Glu Leu Leu Ala Lys Ala Lys Ala Ala Asn Ile Ile Leu Glu Val145 150 155 160Glu Ile Gly Val Val Gly Gly Glu Glu Asp Gly Val Glu Ala Lys Ala
165 170 175Gly Ala Asn Leu Tyr Thr Ser Pro Glu Asp Phe Glu Lys Thr Ile Asp
180 185 190Ala Ile Gly Thr Gly Glu Lys Gly Arg Tyr Leu Leu Ala Ala Thr Phe
195 200 205Gly Asn Val His Gly Val Tyr Lys Pro Gly Asn Val Lys Leu Arg Pro
210 215 220Glu Val Leu Leu Glu Gly Gln Gln Val Ala Arg Lys Lys Leu Gly Leu225 230 235 240Ala Asp Asp Ala Leu Pro Phe Asp Phe Val Phe His Gly Gly Ser Gly
245 250 255Ser Glu Lys Glu Lys Ile Glu Glu Ala Leu Thr Tyr Gly Val Ile Lys
260 265 270Met Asn Val Asp Thr Asp Thr Gln Tyr Ala Phe Thr Arg Pro Ile Val
275 280 285Ser His Met Phe Glu Asn Tyr Asn Gly Val Leu Lys Ile Asp Gly Glu
290 295 300Val Gly Asn Lys Lys Ala Tyr Asp Pro Arg Ser Tyr Met Lys Lys Ala305 310 315 320Glu Gln Ser Met Ser Glu Arg Ile Ile Glu Ser Cys Gln Asp Leu Lys
325 330 335Ser Val Gly Lys Thr Thr Ser Lys
340<210>61<211>900<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(877)<223>RXA02258<400>61cgttgcaatt ctcgctcagt aaatccgaca cggccctttg ttagaaaaca aaacataaag 60ggccaccggg aaactttttt aagaaaggtg tgtttcacac atg gca cgt aag cca 115
Met Ala Arg Lys Pro
1 5ctt atc gct ggt aac tgg aag atg aac ctg gat cac cag cag gca atc 163Leu Ile Ala Gly Asn Trp Lys Met Asn Leu Asp His Gln Gln Ala Ile
10 15 20ggc act gtt cag aag ctt gca ttc gcc ctt cca aag gaa tac ttc gag 211Gly Thr Val Gln Lys Leu Ala Phe Ala Leu Pro Lys Glu Tyr Phe Glu
25 30 35aag gtt gac gtt gca gtc acc gtt cct ttc act gac atc cgc tcc gtc 259Lys Val Asp Val Ala Val Thr Val Pro Phe Thr Asp Ile Arg Ser Val
40 45 50cag act ctc gtt gag ggc gac aag ctt gag gtc act ttc ggt gct cag 307Gln Thr Leu Val Glu Gly Asp Lys Leu Glu Val Thr Phe Gly Ala Gln
55 60 65gac gtc tcc cag cac gag tcc ggt gcg tac acc ggt gaa gtt tct gca 355Asp Val Ser Gln His Glu Ser Gly Ala Tyr Thr Gly Glu Val Ser Ala70 75 80 85agc atg ctg gca aag ttg aac tgc tct tgg gtt gtc gtt gga cac tcc 403Ser Met Leu Ala Lys Leu Asn Cys Ser Trp Val Val Val Gly His Ser
90 95 100gag cgc cgc gag tac cac aac gag tct gat gag ttg gtt gct gcg aag 451Glu Arg Arg Glu Tyr His Asn Glu Ser Asp Glu Leu Val Ala Ala Lys
105 110 115gca aag gca gct ctg tcc aac ggc atc agc ccg atc gtc tgc gtt ggt 499Ala Lys Ala Ala Leu Ser Asn Gly Ile Ser Pro Ile Val Cys Val Gly
120 125 130gag cca ctg gaa atc cgt gaa gct ggc acc cac gtt gag tac gtc gtc 547Glu Pro Leu Glu Ile Arg Glu Ala Gly Thr His Val Glu Tyr Val Val
135 140 145gag cag acc cgt aag tcc ctt gct ggc ctg gat gct gct gag ctg gcc 595Glu Gln Thr Arg Lys Ser Leu Ala Gly Leu Asp Ala Ala Glu Leu Ala150 155 160 165aac acc gtt atc gcg tat gag cca gtg tgg gct atc ggc acc ggt aag 643Asn Thr Val Ile Ala Tyr Glu Pro Val Trp Ala Ile Gly Thr Gly Lys
170 175 180gtt gct tcc gca gct gac gct cag gaa gtg tgc aag gct atc cgc ggt 691Val Ala Ser Ala Ala Asp Ala Gln Glu Val Cys Lys Ala Ile Arg Gly
185 190 195ctg atc gtg gag ctt gca ggc gac gag gtc gct gag ggc ctg cgt att 739Leu Ile Val Glu Leu Ala Gly Asp Glu Val Ala Glu Gly Leu Arg Ile
200 205 210ctt tac ggt ggt tct gtt aag gca gaa acc gtc gct gag atc gtc ggt 787Leu Tyr Gly Gly Ser Val Lys Ala Glu Thr Val Ala Glu Ile Val Gly
215 220 225cag cct gac gtc gac ggc gga ctt gtc ggt ggc gct tcc ctc gac ggt 835Gln Pro Asp Val Asp Gly Gly Leu Val Gly Gly Ala Ser Leu Asp Gly230 235 240 245gaa gca ttc gcc aag ctg gct gcc aac gct gcg agc gtt gct 877Glu Ala Phe Ala Lys Leu Ala Ala Asn Ala Ala Ser Val Ala
250 255taaagtacag agctttaaag cac 900<210>62<211>259<212>PRT<213>谷氨酸棒杆菌<400> 62Met Ala Arg Lys Pro Leu Ile Ala Gly Asn Trp Lys Met Asn Leu Asp1 5 10 15His Gln Gln Ala Ile Gly Thr Val Gln Lys Leu Ala Phe Ala Leu Pro
20 25 30Lys Glu Tyr Phe Glu Lys Val Asp Val Ala Val Thr Val Pro Phe Thr
35 40 45Asp Ile Arg Ser Val Gln Thr Leu Val Glu Gly Asp Lys Leu Glu Val
50 55 60Thr Phe Gly Ala Gln Asp Val Ser Gln His Glu Ser Gly Ala Tyr Thr65 70 75 80Gly Glu Val Ser Ala Ser Met Leu Ala Lys Leu Asn Cys Ser Trp Val
85 90 95Val Val Gly His Ser Glu Arg Arg Glu Tyr His Asn Glu Ser Asp Glu
100 105 110Leu Val Ala Ala Lys Ala Lys Ala Ala Leu Ser Asn Gly Ile Ser Pro
115 120 125Ile Val Cys Val Gly Glu Pro Leu Glu Ile Arg Glu Ala Gly Thr His
130 135 140Val Glu Tyr Val Val Glu Gln Thr Arg Lys Ser Leu Ala Gly Leu Asp145 150 155 160Ala Ala Glu Leu Ala Asn Thr Val Ile Ala Tyr Glu Pro Val Trp Ala
165 170 175Ile Gly Thr Gly Lys Val Ala Ser Ala Ala Asp Ala Gln Glu Val Cys
180 185 190Lys Ala Ile Arg Gly Leu Ile Val Glu Leu Ala Gly Asp Glu Val Ala
195 200 205Glu Gly Leu Arg Ile Leu Tyr Gly Gly Ser Val Lys Ala Glu Thr Val
210 215 220Ala Glu Ile Val Gly Gln Pro Asp Val Asp Gly Gly Leu Val Gly Gly225 230 235 240Ala Ser Leu Asp Gly Glu Ala Phe Ala Lys Leu Ala Ala Asn Ala Ala
245 250 255Ser Val Ala<210>63<211>1563<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1540)<223>RXN01225<400>63tttgggctaa tgttgggggg agtgctttca actatccacg agagctgccc agtgataaac 60cccgggttaa ccccacgcct aagtcagtga aggacttttt atg acg cac aac cac 115
Met Thr His Asn His
1 5aag gac tgg aac gat cgc att gca gtt gcg gag gaa atg gtg ccg ttg 163Lys Asp Trp Asn Asp Arg Ile Ala Val Ala Glu Glu Met Val Pro Leu
10 15 20atc ggg cgc ctg cac cgc aac aac aac gtg gtg gtt tcc gta ttc ggt 211Ile Gly Arg Leu His Arg Asn Asn Asn Val Val Val Ser Val Phe Gly
25 30 35cgt ctc ctt gtg aat gtc tca gac atc gat atc atc aag tct cac cgc 259Arg Leu Leu Val Asn Val Ser Asp Ile Asp Ile Ile Lys Ser His Arg
40 45 50tac gcc cgc cac atc ata tcc aag gaa ctt cca ctg gaa agc tcc ttg 307Tyr Ala Arg His Ile Ile Ser Lys Glu Leu Pro Leu Glu Ser Ser Leu
55 60 65gat att ttg cgc gaa ctg gta gat atg aac ctt ggt acc gca tcg atc 355Asp Ile Leu Arg Glu Leu Val Asp Met Asn Leu Gly Thr Ala Ser Ile70 75 80 85gac ctg gga cag ctg gcc tac agc ttc gaa gaa tcc gaa agc acc gac 403Asp Leu Gly Gln Leu Ala Tyr Ser Phe Glu Glu Ser Glu Ser Thr Asp
90 95 100ctg cgt gcc ttc ctg gag gac gct ctc gcg ccg gtc att ggt gcg gaa 451Leu Arg Ala Phe Leu Glu Asp Ala Leu Ala Pro Val Ile Gly Ala Glu
105 110 115acc gac atc aac cca act gat atc gtg ctg tac ggt ttc ggc cgc atc 499Thr Asp Ile Asn Pro Thr Asp Ile Val Leu Tyr Gly Phe Gly Arg Ile
120 125 130ggt cgc ctg ctg gcc cgc atc ctg gtt tcc cgc gag gca ctg tat gac 547Gly Arg Leu Leu Ala Arg Ile Leu Val Ser Arg Glu Ala Leu Tyr Asp
135 140 145ggt gct cgt ctg cgc gcc atc gtg gtc cgc aaa aat ggt gaa gaa gac 595Gly Ala Arg Leu Arg Ala Ile Val Val Arg Lys Asn Gly Glu Glu Asp150 155 160 165ctg gtc aag cgc gca tcc ttg ctg cgt cgt gat tct gtc cac ggt gga 643Leu Val Lys Arg Ala Ser Leu Leu Arg Arg Asp Ser Val His Gly Gly
170 175 180ttc gat ggc acc atc acc acc gat tat gac aac aac atc atc tgg gcc 691Phe Asp Gly Thr Ile Thr Thr Asp Tyr Asp Asn Asn Ile Ile Trp Ala
185 190 195aac ggc acc cca atc aag gtc atc tac tcc aat gac cca gcc acc att 739Asn Gly Thr Pro Ile Lys Val Ile Tyr Ser Asn Asp Pro Ala Thr Ile
200 205 210gat tac acc gaa tac ggc atc aat gac gcc gtc gtg gta gac aac acc 787Asp Tyr Thr Glu Tyr Gly Ile Asn Asp Ala Val Val Val Asp Asn Thr
215 220 225ggc cgc tgg cgt gac cgc gaa ggc ctg tcc cag cac ctc aag tcc aag 835Gly Arg Trp Arg Asp Arg Glu Gly Leu Ser Gln His Leu Lys Ser Lys230 235 240 245ggc gtt gcc aag gtt gta ctc acc gcg ccg ggc aag ggc gat ctg aag 883Gly Val Ala Lys Val Val Leu Thr Ala Pro Gly Lys Gly Asp Leu Lys
250 255 260aac atc gtg tac ggc atc aac cac acc gac atc acc gca gat gat cag 93lAsn Ile Val Tyr Gly Ile Asn His Thr Asp Ile Thr Ala Asp Asp Gln
265 270 275atc gtt tcc gca gca tca tgc acc acc aat gcc att acc cca gtg ctc 979Ile Val Ser Ala Ala Ser Cys Thr Thr Asn Ala Ile Thr Pro Val Leu
280 285 290aag gtg atc aat gat cgc tac ggc gtg gaa ttc ggc cac gta gaa acc 1027Lys Val Ile Asn Asp Arg Tyr Gly Val Glu Phe Gly His Val Glu Thr
295 300 305gtt cac tcc ttc acc aat gac cag aac ctg atc gac aac ttc cac aag 1075Val His Ser Phe Thr Asn Asp Gln Asn Leu Ile Asp Asn Phe His Lys310 315 320 325ggt tct cgc cgt ggt cgc gca gca ggt ctg aat atg gtt ctc acc gaa 1123Gly Ser Arg Arg Gly Arg Ala Ala Gly Leu Asn Met Val Leu Thr Glu
330 335 340acc ggc gct gca aag gct gta tcc aag gcg ctt cca gag ctg gaa ggc 1171Thr Gly Ala Ala Lys Ala Val Ser Lys Ala Leu Pro Glu Leu Glu Gly
345 350 355aag ctc acc ggc aat gcc atc cgc gtt cct acc cct gac gtg tcc atg 1219Lys Leu Thr Gly Asn Ala Ile Arg Val Pro Thr Pro Asp Val Ser Met
360 365 370gct gtg ctc aac ttg acc ctg aac acg gag gtg gac cgc gat gag gtc 1267Ala Val Leu Asn Leu Thr Leu Asn Thr Glu Val Asp Arg Asp Glu Val
375 380 385aac gag ttc ctc cgc cgt gtg tcc ctg cac tct gac ttg cgc cag caa 1315Asn Glu Phe Leu Arg Arg Val Ser Leu His Ser Asp Leu Arg Gln Gln390 395 400 405atc gac tgg atc cgt tcc cca gag gtt gtt tcc act gac ttc gtg ggc 1363Ile Asp Trp Ile Arg Ser Pro Glu Val Val Ser Thr Asp Phe Val Gly
410 415 420acc acc cac gcg ggc atc gtt gat ggt cta gcc acc atc gca acc ggt 1411Thr Thr His Ala Gly Ile Val Asp Gly Leu Ala Thr Ile Ala Thr Gly
425 430 435cgc cac ctg gtg ctt tac gtg tgg tac gac aac gag ttc ggc tac tcc 1459Arg His Leu Val Leu Tyr Val Trp Tyr Asp Asn Glu Phe Gly Tyr Ser
440 445 450aac cag gtc att cgc atc gtc gag gag atc gcc ggc gtg cgt cct cgc 1507Asn Gln Val Ile Arg Ile Val Glu Glu Ile Ala Gly Val Arg Pro Arg
455 460 465gtg tac ccg gag cgc agg cag cca gcc gta cta taggttatcc aagcctaata 1560Val Tyr Pro Glu Arg Arg Gln Pro Ala Val Leu470 475 480cac 1563<210>64<211>480<212>PRT<213>谷氨酸棒杆菌<400>64Met Thr His Asn His Lys Asp Trp Asn Asp Arg Ile Ala Val Ala Glu1 5 10 15Glu Met Val Pro Leu Ile Gly Arg Leu His Arg Asn Asn Asn Val Val
20 25 30Val Ser Val Phe Gly Arg Leu Leu Val Asn Val Ser Asp Ile Asp Ile
35 40 45Ile Lys Ser His Arg Tyr Ala Arg His Ile Ile Ser Lys Glu Leu Pro
50 55 60Leu Glu Ser Ser Leu Asp Ile Leu Arg Glu Leu Val Asp Met Asn Leu65 70 75 80Gly Thr Ala Ser Ile Asp Leu Gly Gln Leu Ala Tyr Ser Phe Glu Glu
85 90 95Ser Glu Ser Thr Asp Leu Arg Ala Phe Leu Glu Asp Ala Leu Ala Pro
100 105 110Val Ile Gly Ala Glu Thr Asp Ile Asn Pro Thr Asp Ile Val Leu Tyr
115 120 125Gly Phe Gly Arg Ile Gly Arg Leu Leu Ala Arg Ile Leu Val Ser Arg
130 135 140Glu Ala Leu Tyr Asp Gly Ala Arg Leu Arg Ala Ile Val Val Arg Lys145 150 155 160Asn Gly Glu Glu Asp Leu Val Lys Arg Ala Ser Leu Leu Arg Arg Asp
165 170 175Ser Val His Gly Gly Phe Asp Gly Thr Ile Thr Thr Asp Tyr Asp Asn
180 185 190Asn Ile Ile Trp Ala Asn Gly Thr Pro Ile Lys Val Ile Tyr Ser Asn
195 200 205Asp Pro Ala Thr Ile Asp Tyr Thr Glu Tyr Gly Ile Asn Asp Ala Val
210 215 220Val Val Asp Asn Thr Gly Arg Trp Arg Asp Arg Glu Gly Leu Ser Gln225 230 235 240His Leu Lys Ser Lys Gly Val Ala Lys Val Val Leu Thr Ala Pro Gly
245 250 255Lys Gly Asp Leu Lys Asn Ile Val Tyr Gly Ile Asn His Thr Asp Ile
260 265 270Thr Ala Asp Asp Gln Ile Val Ser Ala Ala Ser Cys Thr Thr Asn Ala
275 280 285Ile Thr Pro Val Leu Lys Val Ile Asn Asp Arg Tyr Gly Val Glu Phe
290 295 300Gly His Val Glu Thr Val His Ser Phe Thr Asn Asp Gln Asn Leu Ile305 310 315 320Asp Asn Phe His Lys Gly Ser Arg Arg Gly Arg Ala Ala Gly Leu Asn
325 330 335Met Val Leu Thr Glu Thr Gly Ala Ala Lys Ala Val Ser Lys Ala Leu
340 345 350Pro Glu Leu Glu Gly Lys Leu Thr Gly Asn Ala Ile Arg Val Pro Thr
355 360 365Pro Asp Val Ser Met Ala Val Leu Asn Leu Thr Leu Asn Thr Glu Val
370 375 380Asp Arg Asp Glu Val Asn Glu Phe Leu Arg Arg Val Ser Leu His Ser385 390 395 400Asp Leu Arg Gln Gln Ile Asp Trp Ile Arg Ser Pro Glu Val Val Ser
405 410 415Thr Asp Phe Val Gly Thr Thr His Ala Gly Ile Val Asp Gly Leu Ala
420 425 430Thr Ile Ala Thr Gly Arg His Leu Val Leu Tyr Val Trp Tyr Asp Asn
435 440 445Glu Phe Gly Tyr Ser Asn Gln Val Ile Arg Ile Val Glu Glu Ile Ala
450 455 460Gly Val Arg Pro Arg Val Tyr Pro Glu Arg Arg Gln Pro Ala Val Leu465 470 475 480<210>65<211>1563<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1540)<223>FRXA01225<400>65tttgggctaa tgttgggggg agtgctttca actatccacg agagctgccc agtgataaac 60cccgggttaa ccccacgcct aagtcagtga aggacttttt atg acg cac aac cac 115
Met Thr His Asn His
1 5aag gac tgg aac gat cgc att gca gtt gcg gag gaa atg gtg ccg ttg 163Lys Asp Trp Asn Asp Arg Ile Ala Val Ala Glu Glu Met Val Pro Leu
10 15 20atc ggg cgc ctg cac cgc aac aac aac gtg gtg gtt tcc gta ttc ggt 211Ile Gly Arg Leu His Arg Asn Asn Asn Val Val Val Ser Val Phe Gly
25 30 35cgt ctc ctt gtg aat gtc tca gac atc gat atc atc aag tct cac cgc 259Arg Leu Leu Val Asn Val Ser Asp Ile Asp Ile Ile Lys Ser His Arg
40 45 50tac gcc cgc cac atc ata tcc aag gaa ctt cca ctg gaa agc tcc ttg 307Tyr Ala Arg His Ile Ile Ser Lys Glu Leu Pro Leu Glu Ser Ser Leu
55 60 65gat att ttg cgc gaa ctg gta gat atg aac ctt ggt acc gca tcg atc 355Asp Ile Leu Arg Glu Leu Val Asp Met Asn Leu Gly Thr Ala Ser Ile70 75 80 85gac ctg gga cag ctg gcc tac agc ttc gaa gaa tcc gaa agc acc gac 403Asp Leu Gly Gln Leu Ala Tyr Ser Phe Glu Glu Ser Glu Ser Thr Asp
90 95 100ctg cgt gcc ttc ctg gag gac gct ctc gcg ccg gtc att ggt gcg gaa 451Leu Arg Ala Phe Leu Glu Asp Ala Leu Ala Pro Val Ile Gly Ala Glu
105 110 115acc gac atc aac cca act gat atc gtg ctg tac ggt ttc ggc cgc atc 499Thr Asp Ile Asn Pro Thr Asp Ile Val Leu Tyr Gly Phe Gly Arg Ile
120 125 130ggt cgc ctg ctg gcc cgc atc ctg gtt tcc cgc gag gca ctg tat gac 547Gly Arg Leu Leu Ala Arg Ile Leu Val Ser Arg Glu Ala Leu Tyr Asp
135 140 145ggt gct cgt ctg cgc gcc atc gtg gtc cgc aaa aat ggt gaa gaa gac 595Gly Ala Arg Leu Arg Ala Ile Val Val Arg Lys Asn Gly Glu Glu Asp150 155 160 165ctg gtc aag cgc gca tcc ttg ctg cgt cgt gat tct gtc cac ggt gga 643Leu Val Lys Arg Ala Ser Leu Leu Arg Arg Asp Ser Val His Gly Gly
170 175 180ttc gat ggc acc atc acc acc gat tat gac aac aac atc atc tgg gcc 691Phe Asp Gly Thr Ile Thr Thr Asp Tyr Asp Asn Asn Ile Ile Trp Ala
185 190 195aac ggc acc cca atc aag gtc atc tac tcc aat gac cca gcc acc att 739Asn Gly Thr Pro Ile Lys Val Ile Tyr Ser Asn Asp Pro Ala Thr Ile
200 205 210gat tac acc gaa tac ggc atc aat gac gcc gtc gtg gta gac aac acc 787Asp Tyr Thr Glu Tyr Gly Ile Asn Asp Ala Val Val Val Asp Asn Thr
215 220 225ggc cgc tgg cgt gac cgc gaa ggc ctg tcc cag cac ctc aag tcc aag 835Gly Arg Trp Arg Asp Arg Glu Gly Leu Ser Gln His Leu Lys Ser Lys230 235 240 245ggc gtt gcc aag gtt gta ctc acc gcg ccg ggc aag ggc gat ctg aag 883Gly Val Ala Lys Val Val Leu Thr Ala Pro Gly Lys Gly Asp Leu Lys
250 255 260aac atc gtg tac ggc atc aac cac acc gac atc acc gca gat gat cag 931Asn Ile Val Tyr Gly Ile Asn His Thr Asp Ile Thr Ala Asp Asp Gln
265 270 275atc gtt tcc gca gca tca tgc acc acc aat gcc att acc cca gtg ctc 979Ile Val Ser Ala Ala Ser Cys Thr Thr Asn Ala Ile Thr Pro Val Leu
280 285 290aag gtg atc aat gat cgc tac ggc gtg gaa ttc ggc cac gta gaa acc 1027Lys Val Ile Asn Asp Arg Tyr Gly Val Glu Phe Gly His Val Glu Thr
295 300 305gtt cac tcc ttc acc aat gac cag aac ctg atc gac aac ttc cac aag 1075Val His Ser Phe Thr Asn Asp Gln Asn Leu Ile Asp Asn Phe His Lys310 315 320 325ggt tct cgc cgt ggt cgc gca gca ggt ctg aat atg gtt ctc acc gaa 1123Gly Ser Arg Arg Gly Arg Ala Ala Gly Leu Asn Met Val Leu Thr Glu
330 335 340acc ggc gct gca aag gct gta tcc aag gcg ctt cca gag ctg gaa ggc 1171Thr Gly Ala Ala Lys Ala Val Ser Lys Ala Leu Pro Glu Leu Glu Gly
345 350 355aag ctc acc ggc aat gcc atc cgc gtt cct acc cct gac gtg tcc atg 1219Lys Leu Thr Gly Asn Ala Ile Arg Val Pro Thr Pro Asp Val Ser Met
360 365 370gct gtg ctc aac ttg acc ctg aac acg gag gtg gac cgc gat gag gtc 1267Ala Val Leu Asn Leu Thr Leu Asn Thr Glu Val Asp Arg Asp Glu Val
375 380 385aac gag ttc ctc cgc cgt gtg tcc ctg cac tct gac ttg cgc cag caa 1315Asn Glu Phe Leu Arg Arg Val Ser Leu His Ser Asp Leu Arg Gln Gln390 395 400 405atc gac tgg atc cgt tcc cca gag gtt gtt tcc act gac ttc gtg ggc 1363Ile Asp Trp Ile Arg Ser Pro Glu Val Val Ser Thr Asp Phe Val Gly
410 415 420acc acc cac gcg ggc atc gtt gat ggt cta gcc acc atc gca acc ggt 1411Thr Thr His Ala Gly Ile Val Asp Gly Leu Ala Thr Ile Ala Thr Gly
425 430 435cgc cac ctg gtg ctt tac gtg tgg tac gac aac gag ttc ggc tac tcc 1459Arg His Leu Val Leu Tyr Val Trp Tyr Asp Asn Glu Phe Gly Tyr Ser
440 445 450aac cag gtc att cgc atc gtc gag gag atc gcc ggc gtg cgt cct cgc 1507Asn Gln Val Ile Arg Ile Val Glu Glu Ile Ala Gly Val Arg Pro Arg
455 460 465gtg tac ccg gag cgc agg cag cca gcc gta cta taggttatcc aagcctaata 1560Val Tyr Pro Glu Arg Arg Gln Pro Ala Val Leu470 475 480cac 1563<210>66<211>480<212>PRT<213>谷氨酸棒杆菌<400>66Met Thr His Asn His Lys Asp Trp Asn Asp Arg Ile Ala Val Ala Glu1 5 10 15Glu Met Val Pro Leu Ile Gly Arg Leu His Arg Asn Asn Asn Val Val
20 25 30Val Ser Val Phe Gly Arg Leu Leu Val Asn Val Ser Asp Ile Asp Ile
35 40 45Ile Lys Ser His Arg Tyr Ala Arg His Ile Ile Ser Lys Glu Leu Pro
50 55 60Leu Glu Ser Ser Leu Asp Ile Leu Arg Glu Leu Val Asp Met Asn Leu65 70 75 80Gly Thr Ala Ser Ile Asp Leu Gly Gln Leu Ala Tyr Ser Phe Glu Glu
85 90 95Ser Glu Ser Thr Asp Leu Arg Ala Phe Leu Glu Asp Ala Leu Ala Pro
100 105 110Val Ile Gly Ala Glu Thr Asp Ile Asn Pro Thr Asp Ile Val Leu Tyr
115 120 125Gly Phe Gly Arg Ile Gly Arg Leu Leu Ala Arg Ile Leu Val Ser Arg
130 135 140Glu Ala Leu Tyr Asp Gly Ala Arg Leu Arg Ala Ile Val Val Arg Lys145 150 155 160Asn Gly Glu Glu Asp Leu Val Lys Arg Ala Ser Leu Leu Arg Arg Asp
165 170 175Ser Val His Gly Gly Phe Asp Gly Thr Ile Thr Thr Asp Tyr Asp Asn
180 185 190Asn Ile Ile Trp Ala Asn Gly Thr Pro Ile Lys Val Ile Tyr Ser Asn
195 200 205Asp Pro Ala Thr Ile Asp Tyr Thr Glu Tyr Gly Ile Asn Asp Ala Val
210 215 220Val Val Asp Asn Thr Gly Arg Trp Arg Asp Arg Glu Gly Leu Ser Gln225 230 235 240His Leu Lys Ser Lys Gly Val Ala Lys Val Val Leu Thr Ala Pro Gly
245 250 255Lys Gly Asp Leu Lys Asn Ile Val Tyr Gly Ile Asn His Thr Asp Ile
260 265 270Thr Ala Asp Asp Gln Ile Val Ser Ala Ala Ser Cys Thr Thr Asn Ala
275 280 285Ile Thr Pro Val Leu Lys Val Ile Asn Asp Arg Tyr Gly Val Glu Phe
290 295 300Gly His Val Glu Thr Val His Ser Phe Thr Asn Asp Gln Asn Leu Ile305 310 315 320Asp Asn Phe His Lys Gly Ser Arg Arg Gly Arg Ala Ala Gly Leu Asn
325 330 335Met Val Leu Thr Glu Thr Gly Ala Ala Lys Ala Val Ser Lys Ala Leu
340 345 350Pro Glu Leu Glu Gly Lys Leu Thr Gly Asn Ala Ile Arg Val Pro Thr
355 360 365Pro Asp Val Ser Met Ala Val Leu Asn Leu Thr Leu Asn Thr Glu Val
370 375 380Asp Arg Asp Glu Val Asn Glu Phe Leu Arg Arg Val Ser Leu His Ser385 390 395 400Asp Leu Arg Gln Gln Ile Asp Trp Ile Arg Ser Pro Glu Val Val Ser
405 410 415Thr Asp Phe Val Gly Thr Thr His Ala Gly Ile Val Asp Gly Leu Ala
420 425 430Thr Ile Ala Thr Gly Arg His Leu Val Leu Tyr Val Trp Tyr Asp Asn
435 440 445Glu Phe Gly Tyr Ser Asn Gln Val Ile Arg Ile Val Glu Glu Ile Ala
450 455 460Gly Val Arg Pro Arg Val Tyr Pro Glu Arg Arg Gln Pro Ala Val Leu465 470 475 480<210>67<211>1125<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1102)<223>RXA02256<400>67ttgggttctg tcagctcaag aattcttgag tgaccgatgc tctgattgac ctaactgctt 60gacacattgc atttcctaca atctttagag gagacacaac atg acc att cgt gtt 115
Met Thr Ile Arg Val
1 5ggt att aac gga ttt ggc cgt atc gga cgt aac ttc ttc cgc gca gtt 163Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Asn Phe Phe Arg Ala Val
10 15 20ctg gag cgc agc gac gat ctc gag gta gtt gca gtc aac gac ctc acc 211Leu Glu Arg Ser Asp Asp Leu Glu Val Val Ala Val Asn Asp Leu Thr
25 30 35gac aac aag acc ctt tcc acc ctt ctc aag ttc gac tcc atc atg ggc 259Asp Asn Lys Thr Leu Ser Thr Leu Leu Lys Phe Asp Ser Ile Met Gly
40 45 50cgc ctt ggc cag gaa gtt gaa tac gac gat gac tcc atc acc gtt ggt 307Arg Leu Gly Gln Glu Val Glu Tyr Asp Asp Asp Ser Ile Thr Val Gly
55 60 65ggc aag cgc atc gct gtt tac gca gag cgc gat cca aag aac ctg gac 355Gly Lys Arg Ile Ala Val Tyr Ala Glu Arg Asp Pro Lys Asn Leu Asp70 75 80 85tgg gct gca cac aac gtt gac atc gtg atc gag tcc acc ggc ttc ttc 403Trp Ala Ala His Asn Val Asp Ile Val Ile Glu Ser Thr Gly Phe Phe
90 95 100acc gat gca aac gcg gct aag gct cac atc gaa gca ggt gcc aag aag 451Thr Asp Ala Asn Ala Ala Lys Ala His Ile Glu Ala Gly Ala Lys Lys
105 110 115gtc atc atc tcc gca cca gca agc aac gaa gac gca acc ttc gtt tac 499Val Ile Ile Ser Ala Pro Ala Ser Asn Glu Asp Ala Thr Phe Val Tyr
120 125 130ggt gtg aac cac gag tcc tac gat cct gag aac cac aac gtg atc tcc 547Gly Val Asn His Glu Ser Tyr Asp Pro Glu Asn His Asn Val Ile Ser
135 140 145ggc gca tct tgc acc acc aac tgc ctc gca cca atg gca aag gtc cta 595Gly Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro Met Ala Lys Val Leu150 155 160 165aac gac aag ttc ggc atc gag aac ggc ctc atg acc acc gtt cac gca 643Asn Asp Lys Phe Gly Ile Glu Asn Gly Leu Met Thr Thr Val His Ala
170 175 180tac act ggc gac cag cgc ctg cac gat gca cct cac cgc gac ctg cgt 691Tyr Thr Gly Asp Gln Arg Leu His Asp Ala Pro His Arg Asp Leu Arg
185 190 195cgt gca cgt gca gca gca gtc aac atc gtt cct acc tcc acc ggt gca 739Arg Ala Arg Ala Ala Ala Val Asn Ile Val Pro Thr Ser Thr Gly Ala
200 205 210gct aag gct gtc gct ctg gtt ctc cca gag ctc aag ggc aag ctt gac 787Ala Lys Ala Val Ala Leu Val Leu Pro Glu Leu Lys Gly Lys Leu Asp215 220 225ggc tac gca ctt cgc gtt cca gtt atc acc ggt tcc gca acc gac ccg 835Gly Tyr Ala Leu Arg Val Pro Val Ile Thr Gly Ser Ala Thr Asp Leu230 235 240 245acc ttc aac acc aag tct gag gtc acc gtt gag tcc atc aac gct gca 883Thr Phe Asn Thr Lys Ser Glu Val Thr Val Glu Ser Ile Asn Ala Ala
250 255 260atc aag gaa gct gca gtc ggc gag ttc ggc gag acc ctg gct tac tcc 931Ile Lys Glu Ala Ala Val Gly Glu Phe Gly Glu Thr Leu Ala Tyr Ser
265 270 275gaa gag cca ctg gtt tcc acc gac atc gtc cac gat tcc cac ggc tcc 979Glu Glu Pro Leu Val Ser Thr Asp Ile Val His Asp Ser His Gly Ser
280 285 290atc ttc gac gct ggc ctg acc aag gtc tcc ggc aac acc gtc aag gtt 1027Ile Phe Asp Ala Gly Leu Thr Lys Val Ser Gly Asn Thr Val Lys Val
295 300 305gtt tcc tgg tac gac aac gag tgg ggc tac acc tgc cag ctc ctg cgt 1075Val Ser Trp Tyr Asp Asn Glu Trp Gly Tyr Thr Cys Gln Leu Leu Arg310 315 320 325ctg acc gag ctc gta gct tcc aag ctc taattagttc acatcgctaa 1122Leu Thr Glu Leu Val Ala Ser Lys Leu
330cgt 1125<210>68<211>334<212>PRT<213>谷氨酸棒杆菌<400>68Met Thr Ile Arg Val Gly Ile Asn Gly Phe Gly Arg Ile Gly Arg Asn1 5 10 15Phe Phe Arg Ala Val Leu Glu Arg Ser Asp Asp Leu Glu Val Val Ala
20 25 30Val Asn Asp Leu Thr Asp Asn Lys Thr Leu Ser Thr Leu Leu Lys Phe
35 40 45Asp Ser Ile Met Gly Arg Leu Gly Gln Glu Val Glu Tyr Asp Asp Asp
50 55 60Ser Ile Thr Val Gly Gly Lys Arg Ile Ala Val Tyr Ala Glu Arg Asp65 70 75 80Pro Lys Asn Leu Asp Trp Ala Ala His Asn Val Asp Ile Val Ile Glu
85 90 95Ser Thr Gly Phe Phe Thr Asp Ala Asn Ala Ala Lys Ala His Ile Glu
100 105 110Ala Gly Ala Lys Lys Val Ile Ile Ser Ala Pro Ala Ser Asn Glu Asp
115 120 125Ala Thr Phe Val Tyr Gly Val Asn His Glu Ser Tyr Asp Pro Glu Asn
130 135 140His Asn Val Ile Ser Gly Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro145 150 155 160Met Ala Lys Val Leu Asn Asp Lys Phe Gly Ile Glu Asn Gly Leu Met
165 170 175Thr Thr Val His Ala Tyr Thr Gly Asp Gln Arg Leu His Asp Ala Pro
180 185 190His Arg Asp Leu Arg Arg Ala Arg Ala Ala Ala Val Asn Ile Val Pro
195 200 205Thr Ser Thr Gly Ala Ala Lys Ala Val Ala Leu Val Leu Pro Glu Leu
210 215 220Lys Gly Lys Leu Asp Gly Tyr Ala Leu Arg Val Pro Val Ile Thr Gly225 230 235 240Ser Ala Thr Asp Leu Thr Phe Asn Thr Lys Ser Glu Val Thr Val Glu
245 250 255Ser Ile Asn Ala Ala Ile Lys Glu Ala Ala Val Gly Glu Phe Gly Glu
260 265 270Thr Leu Ala Tyr Ser Glu Glu Pro Leu Val Ser Thr Asp Ile Val His
275 280 285Asp Ser His Gly Ser Ile Phe Asp Ala Gly Leu Thr Lys Val Ser Gly
290 295 300Asn Thr Val Lys Val Val Ser Trp Tyr Asp Asn Glu Trp Gly Tyr Thr305 310 315 320Cys Gln Leu Leu Arg Leu Thr Glu Leu Val Ala Ser Lys Leu
325 330<210>69<211>1338<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1315)<223>RXA02257<400>69ataccggtgc cagcgccaca caatgtgtgg caatctggga cagtgcatca cattgcacca 60gaagaatttt ttaaacaatc aaatctccaa ggagtacggc atg gct gtt aag acc 115
Met Ala Val Lys Thr
1 5ctc aag gac ttg ctc gac gaa ggc gta gac gga cgc cac gtc atc gtt 163Leu Lys Asp Leu Leu Asp Glu Gly Val Asp Gly Arg His Val Ile Val
10 15 20cga tct gac ttc aat gtt ccc ctc aac gat gac cgc gag atc acc gat 211Arg Ser Asp Phe Asn Val Pro Leu Asn Asp Asp Arg Glu Ile Thr Asp
25 30 35aag ggc cga atc att gcc tcc cta cca acc ctt aaa gca ctg agc gaa 259Lys Gly Arg Ile Ile Ala Ser Leu Pro Thr Leu Lys Ala Leu Ser Glu
40 45 50ggt ggc gca aag gtc atc gtc atg gct cac ctt ggc cgc cca aag ggc 307Gly Gly Ala Lys Val Ile Val Met Ala His Leu Gly Arg Pro Lys Gly
55 60 65gag gtc aac gag aag tac tcc ctc gca cct gtc gct gag gca ctc tcc 355Glu Val Asn Glu Lys Tyr Ser Leu Ala Pro Val Ala Glu Ala Leu Ser70 75 80 85gat gag ctt ggc cag tac gtt gca ctt gcc gca gac gtt gtt ggc gaa 403Asp Glu Leu Gly Gln Tyr Val Ala Leu Ala Ala Asp Val Val Gly Glu
90 95 100gac gca cac gag cgc gca aac ggc ctg acc gag ggc gac atc ctg ctc 451Asp Ala His Glu Arg Ala Asn Gly Leu Thr Glu Gly Asp Ile Leu Leu
105 110 115ctg gag aac gtg cgc ttc gac cca cgc gaa acc tcc aag gac gag gca 499Leu Glu Asn Val Arg Phe Asp Pro Arg Glu Thr Ser Lys Asp Glu Ala
120 125 130gag cgc acc gct ttc gct cag gag ctc gca gct ctt gca gca gac aac 547Glu Arg Thr Ala Phe Ala Gln Glu Leu Ala Ala Leu Ala Ala Asp Asn
135 140 145ggc gca ttc gtt tct gac ggc ttc ggt gtt gtc cac cgc gca cag acc 595Gly Ala Phe Val Ser Asp Gly Phe Gly Val Val His Arg Ala Gln Thr150 155 160 165tcc gtc tac gac att gca aag ttg ctg cca cac tac gct ggc gga ctg 643Ser Val Tyr Asp Ile Ala Lys Leu Leu Pro His Tyr Ala Gly Gly Leu
170 175 180gta gag acc gag att tcc gtt ctg gaa aag atc gca gaa tca cca gag 69lVal Glu Thr Glu Ile Ser Val Leu Glu Lys Ile Ala Glu Ser Pro Glu
185 190 195gca cca tac gta gtg gtt ctc ggt gga tcc aag gtc tct gac aag atc 739Ala Pro Tyr Val Val Val Leu Gly Gly Ser Lys Val Ser Asp Lys Ile
200 205 210ggt gtt att gag gcg ctg gct gcc aag gct gac aag atc atc gtc ggt 787Gly Val Ile Glu Ala Leu Ala Ala Lys Ala Asp Lys Ile Ile Val Gly
215 220 225ggc ggc atg tgc tac acc ttc ctc gca gct cag gga cac aac gtt cag 835Gly Gly Met Cys Tyr Thr Phe Leu Ala Ala Gln Gly His Asn Val Gln230 235 240 245cag tcc ctc ctg cag gaa gaa atg aag gct acc tgc acc gac ctg ctc 883Gln Ser Leu Leu Gln Glu Glu Met Lys Ala Thr Cys Thr Asp Leu Leu
250 255 260gca cgc ttc ggt gac aag atc gtt ctc cca gtt gac ctg gtt gca gca 931Ala Arg Phe Gly Asp Lys Ile Val Leu Pro Val Asp Leu Val Ala Ala
265 270 275tcc gaa ttt aac aag gac gca gag aag cag atc gtt gac ctg gac tcc 979Ser Glu Phe Asn Lys Asp Ala Glu Lys Gln Ile Val Asp Leu Asp Ser
280 285 290atc cca gaa ggc tgg atg tct ctt gac atc gga cca gag tcc gtc aag 1027Ile Pro Glu Gly Trp Met Ser Leu Asp Ile Gly Pro Glu Ser Val Lys
295 300 305aac ttc ggt gag gtt ctc agc acc gct aag acc atc ttc tgg aac ggc 1075Asn Phe Gly Glu Val Leu Ser Thr Ala Lys Thr Ile Phe Trp Asn Gly310 315 320 325cca atg ggc gtg ttc gag ttc gca gca ttc tct gaa ggc acc cgc ggc 1123Pro Met Gly Val Phe Glu Phe Ala Ala Phe Ser Glu Gly Thr Arg Gly
330 335 340atc gcc cag gcc atc atc gat gca act gca ggc aac gac gca ttc tcc 1171Ile Ala Gln Ala Ile Ile Asp Ala Thr Ala Gly Asn Asp Ala Phe Ser
345 350 355gtt gtt ggc ggt ggc gac tcc gca gca tcc gtt cgc gtg ctc ggc ctg 1219Val Val Gly Gly Gly Asp Ser Ala Ala Ser Val Arg Val Leu Gly Leu
360 365 370aac gaa gac ggc ttc tcc cac atc tcc acc ggt ggt ggc gca tcc ctc 1267Asn Glu Asp Gly Phe Ser His Ile Ser Thr Gly Gly Gly Ala Ser Leu
375 380 385gag tac ctt gaa ggc aag gaa ctc cca ggc gtt gca att ctc gct cag 1315Glu Tyr Leu Glu Gly Lys Glu Leu Pro Gly Val Ala Ile Leu Ala Gln390 395 400 405taaatccgac acggcccttt gtt 1338<210>70<211>405<212>PRT<213>谷氨酸棒杆菌<400>70Met Ala Val Lys Thr Leu Lys Asp Leu Leu Asp Glu Gly Val Asp Gly1 5 10 15Arg His Val Ile Val Arg Ser Asp Phe Asn Val Pro Leu Asn Asp Asp
20 25 30Arg Glu Ile Thr Asp Lys Gly Arg Ile Ile Ala Ser Leu Pro Thr Leu
35 40 45Lys Ala Leu Ser Glu Gly Gly Ala Lys Val Ile Val Met Ala His Leu
50 55 60Gly Arg Pro Lys Gly Glu Val Asn Glu Lys Tyr Ser Leu Ala Pro Val65 70 75 80Ala Glu Ala Leu Ser Asp Glu Leu Gly Gln Tyr Val Ala Leu Ala Ala
85 90 95Asp Val Val Gly Glu Asp Ala His Glu Arg Ala Asn Gly Leu Thr Glu
100 105 110Gly Asp Ile Leu Leu Leu Glu Asn Val Arg Phe Asp Pro Arg Glu Thr
115 120 125Ser Lys Asp Glu Ala Glu Arg Thr Ala Phe Ala Gln Glu Leu Ala Ala
130 135 140Leu Ala Ala Asp Asn Gly Ala Phe Val Ser Asp Gly Phe Gly Val Val145 150 155 160His Arg Ala Gln Thr Ser Val Tyr Asp Ile Ala Lys Leu Leu Pro His
165 170 175Tyr Ala Gly Gly Leu Val Glu Thr Glu Ile Ser Val Leu Glu Lys Ile
180 185 190Ala Glu Ser Pro Glu Ala Pro Tyr Val Val Val Leu Gly Gly Ser Lys
195 200 205Val Ser Asp Lys Ile Gly Val Ile Glu Ala Leu Ala Ala Lys Ala Asp
210 215 220Lys Ile Ile Val Gly Gly Gly Met Cys Tyr Thr Phe Leu Ala Ala Gln225 230 235 240Gly His Asn Val Gln Gln Ser Leu Leu Gln Glu Glu Met Lys Ala Thr
245 250 255Cys Thr Asp Leu Leu Ala Arg Phe Gly Asp Lys Ile Val Leu Pro Val
260 265 270Asp Leu Val Ala Ala Ser Glu Phe Asn Lys Asp Ala Glu Lys Gln Ile
275 280 285Val Asp Leu Asp Ser Ile Pro Glu Gly Trp Met Ser Leu Asp Ile Gly
290 295 300Pro Glu Ser Val Lys Asn Phe Gly Glu Val Leu Ser Thr Ala Lys Thr305 310 315 320Ile Phe Trp Asn Gly Pro Met Gly Val Phe Glu Phe Ala Ala Phe Ser
325 330 335Glu Gly Thr Arg Gly Ile Ala Gln Ala Ile Ile Asp Ala Thr Ala Gly
340 345 350Asn Asp Ala Phe Ser Val Val Gly Gly Gly Asp Ser Ala Ala Ser Val
355 360 365Arg Val Leu Gly Leu Asn Glu Asp Gly Phe Ser His Ile Ser Thr Gly
370 375 380Gly Gly Ala Ser Leu Glu Tyr Leu Glu Gly Lys Glu Leu Pro Gly Val385 390 395 400Ala Ile Leu Ala Gln
405<210>71<211>1398<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1375)<223>RXA00235<400>71cgaaacaaga ttcgtgcaac aattgggtgt agacgtgatt gaagacattt gatcacgtga 60ataattctag ttagctccca agttggcata ggaggccaca gtg gct gaa atc atg 115
Val Ala Glu Ile Met
1 5cac gta ttc gct cgc gaa att ctc gac tcc cgc ggt aac cca acc gtc 163His Val Phe Ala Arg Glu Ile Leu Asp Ser Arg Gly Asn Pro Thr Val
10 15 20gag gca gag gtt ttc ctg gat gac ggt tcc cac ggt gtc gca ggt gtt 211Glu Ala Glu Val Phe Leu Asp Asp Gly Ser His Gly Val Ala Gly Val
25 30 35cca tcc ggc gca tcc acc ggc gtc cac gag gct cat gag ctg cgt gac 259Pro Ser Gly Ala Ser Thr Gly Val His Glu Ala His Glu Leu Arg Asp
40 45 50ggt ggc gat cgc tac ctg ggc aag ggc gtt ttg aag gca gtt gaa aac 307Gly Gly Asp Arg Tyr Leu Gly Lys Gly Val Leu Lys Ala Val Glu Asn
55 60 65gtc aac gaa gaa atc ggc gac gag ctc gct ggc cta gag gct gac gat 355Val Asn Glu Glu Ile Gly Asp Glu Leu Ala Gly Leu Glu Ala Asp Asp70 75 80 85cag cgc ctc atc gac gaa gca atg atc aag ctt gat ggc acc gcc aac 403Gln Arg Leu Ile Asp Glu Ala Met Ile Lys Leu Asp Gly Thr Ala Asn
90 95 100aag tcc cgc ctg ggt gca aac gca atc ctt ggt gtt tcc atg gct gtt 451Lys Ser Arg Leu Gly Ala Asn Ala Ile Leu Gly Val Ser Met Ala Val
105 110 115gca aag gct gct gct gat tcc gca ggc ctc cca ctg ttc cgc tac atc 499Ala Lys Ala Ala Ala Asp Ser Ala Gly Leu Pro Leu Phe Arg Tyr Ile
120 125 130ggt gga cca aac gca cac gtt ctt cca gtt cca atg atg aac atc atc 547Gly Gly Pro Asn Ala His Val Leu Pro Val Pro Met Met Asn Ile Ile
135 140 145acc ggt ggc gct cac gct gac tcc ggt gtt gac gtt cag gaa ttc atg 595Thr Gly Gly Ala His Ala Asp Ser Gly Val Asp Val Gln Glu Phe Met150 155 160 165atc gct cca atc ggt gca gag acc ttc tct gag gct ctc cgc aac ggc 643Ile Ala Pro Ile Gly Ala Glu Thr Phe Ser Glu Ala Leu Arg Asn Gly
170 175 180gcg gag gtc tac cac gca ctg aag tcc gtc atc aag gaa aag ggc ctg 691Ala Glu Val Tyr His Ala Leu Lys Ser Val Ile Lys Glu Lys Gly Leu
185 190 195tcc acc gga ctt ggc gat gag ggc ggc ttc gct cct tcc gtc ggc tcc 739Ser Thr Gly Leu Gly Asp Glu Gly Gly Phe Ala Pro Ser Val Gly Ser
200 205 210acc cgt gag gct ctt gac ctt atc gtt gag gca atc gag aag gct ggc 787Thr Arg Glu Ala Leu Asp Leu Ile Val Glu Ala Ile Glu Lys Ala Gly
215 220 225ttc acc cca ggc aag gac atc gct ctt gct ctg gac gtt gct tcc tct 835Phe Thr Pro Gly Lys Asp Ile Ala Leu Ala Leu Asp Val Ala Ser Ser230 235 240 245gag ttc ttc aag gac ggc acc tac cac ttc gaa ggt ggc cag cac tcc 883Glu Phe Phe Lys Asp Gly Thr Tyr His Phe Glu Gly Gly Gln His Ser
250 255 260gca gct gag atg gca aac gtt tac gct gag ctc gtt gac gcg tac cca 931Ala Ala Glu Met Ala Asn Val Tyr Ala Glu Leu Val Asp Ala Tyr Pro
265 270 275atc gtc tcc atc gag gac cca ctg cag gaa gat gac tgg gag ggt tac 979Ile Val Ser Ile Glu Asp Pro Leu Gln Glu Asp Asp Trp Glu Gly Tyr
280 285 290acc aac ctc acc gca acc atc ggc gac aag gtt cag atc gtt ggc gac 1027Thr Asn Leu Thr Ala Thr Ile Gly Asp Lys Val Gln Ile Val Gly Asp
295 300 305gac ttc ttc gtc acc aac cct gag cgc ctg aag gag ggc atc gct aag 1075Asp Phe Phe Val Thr Asn Pro Glu Arg Leu Lys Glu Gly Ile Ala Lys310 315 320 325aag gct gcc aac tcc atc ctg gtt aag gtg aac cag atc ggt acc ctc 1123Lys Ala Ala Asn Ser Ile Leu Val Lys Val Asn Gln Ile Gly Thr Leu
330 335 340acc gag acc ttc gac gct gtc gac atg gct cac cgc gca ggc tac acc 1171Thr Glu Thr Phe Asp Ala Val Asp Met Ala His Arg Ala Gly Tyr Thr
345 350 355tcc atg atg tcc cac cgt tcc ggt gag acc gag gac acc acc att gct 1219Ser Met Met Ser His Arg Ser Gly Glu Thr Glu Asp Thr Thr Ile Ala
360 365 370gac ctc gca gtt gca ctc aac tgt ggc cag atc aag act ggt gct cca 1267Asp Leu Ala Val Ala Leu Asn Cys Gly Gln Ile Lys Thr Gly Ala Pro
375 380 385gca cgt tcc gac cgt gtc gca aag tac aac cag ctt ctc cgc atc gag 1315Ala Arg Ser Asp Arg Val Ala Lys Tyr Asn Gln Leu Leu Arg Ile Glu390 395 400 405cag ctg ctt ggc gac gcc ggc gtc tac gca ggt cgc agc gca ttc cca 1363Gln Leu Leu Gly Asp Ala Gly Val Tyr Ala Gly Arg Ser Ala Phe Pro
410 415 420cgc ttt cag ggc taaataaaag cgcttttcga cgc 1398Arg Phe Gln Gly
425<210>72<211>425<212>PRT<213>谷氨酸棒杆菌<400>72Val Ala Glu Ile Met His Val Phe Ala Arg Glu Ile Leu Asp Ser Arg1 5 10 15Gly Asn Pro Thr Val Glu Ala Glu Val Phe Leu Asp Asp Gly Ser His
20 25 30Gly Val Ala Gly Val Pro Ser Gly Ala Ser Thr Gly Val His Glu Ala
35 40 45His Glu Leu Arg Asp Gly Gly Asp Arg Tyr Leu Gly Lys Gly Val Leu
50 55 60Lys Ala Val Glu Asn Val Asn Glu Glu Ile Gly Asp Glu Leu Ala Gly65 70 75 80Leu Glu Ala Asp Asp Gln Arg Leu Ile Asp Glu Ala Met Ile Lys Leu
85 90 95Asp Gly Thr Ala Asn Lys Ser Arg Leu Gly Ala Asn Ala Ile Leu Gly
100 105 110Val Ser Met Ala Val Ala Lys Ala Ala Ala Asp Ser Ala Gly Leu Pro
115 120 125Leu Phe Arg Tyr Ile Gly Gly Pro Asn Ala His Val Leu Pro Val Pro
130 135 140Met Met Asn Ile Ile Thr Gly Gly Ala His Ala Asp Ser Gly Val Asp145 150 155 160Val Gln Glu Phe Met Ile Ala Pro Ile Gly Ala Glu Thr Phe Ser Glu
165 170 175Ala Leu Arg Asn Gly Ala Glu Val Tyr His Ala Leu Lys Ser Val Ile
180 185 190Lys Glu Lys Gly Leu Ser Thr Gly Leu Gly Asp Glu Gly Gly Phe Ala
195 200 205Pro Sar Val Gly Ser Thr Arg Glu Ala Leu Asp Leu Ile Val Glu Ala
210 215 220Ile Glu Lys Ala Gly Phe Thr Pro Gly Lys Asp Ile Ala Leu Ala Leu225 230 235 240Asp Val Ala Ser Ser Glu Phe Phe Lys Asp Gly Thr Tyr His Phe Glu
245 250 255Gly Gly Gln His Ser Ala Ala Glu Met Ala Asn Val Tyr Ala Glu Leu
260 265 270Val Asp Ala Tyr Pro Ile Val Ser Ile Glu Asp Pro Leu Gln Glu Asp
275 280 285Asp Trp Glu Gly Tyr Thr Asn Leu Thr Ala Thr Ile Gly Asp Lys Val
290 295 300Gln Ile Val Gly Asp Asp Phe Phe Val Thr Asn Pro Glu Arg Leu Lys305 310 315 320Glu Gly Ile Ala Lys Lys Ala Ala Asn Ser Ile Leu Val Lys Val Asn
325 330 335Gln Ile Gly Thr Leu Thr Glu Thr Phe Asp Ala Val Asp Met Ala His
340 345 350Arg Ala Gly Tyr Thr Ser Met Met Ser His Arg Ser Gly Glu Thr Glu
355 360 365Asp Thr Thr Ile Ala Asp Leu Ala Val Ala Leu Asn Cys Gly Gln Ile
370 375 380Lys Thr Gly Ala Pro Ala Arg Ser Asp Arg Val Ala Lys Tyr Asn Gln385 390 395 400Leu Leu Arg Ile Glu Gln Leu Leu Gly Asp Ala Gly Val Tyr Ala Gly
405 410 415Arg Ser Ala Phe Pro Arg Phe Gln Gly
420 425<210>73<211>1554<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1531)<223>RXA01093<400>73cagaggcgta gaacattgtc tgttcacact ctgggtcgca agattcatcg agaattaatg 60gtagtacctg tggcttgagg gggaatgacg tactaggctt atg ggc gtg gat aga 115
Met Gly Val Asp Arg
1 5cga act aag att gta tgt acc cta ggc cca gcg gtg gct agt gca gat 163Arg Thr Lys Ile Val Cys Thr Leu Gly Pro Ala Val Ala Ser Ala Asp
10 15 20gga att ctg cgt ttg gta gaa gac ggc atg gat gtt gct cgc ctc aac 211Gly Ile Leu Arg Leu Val Glu Asp Gly Met Asp Val Ala Arg Leu Asn
25 30 35ttc tcc cat ggt gac cac cca gat cat gag caa aac tac aag tgg gtc 259Phe Ser His Gly Asp His Pro Asp His Glu Gln Asn Tyr Lys Trp Val
40 45 50cgc gag gcg gcg gag aag act ggc cgt gca gtc ggt att ctc gca gac 307Arg Glu Ala Ala Glu Lys Thr Gly Arg Ala Val Gly Ile Leu Ala Asp
55 60 65ctc caa gga ccg aag atc cgt ctt ggc cgt ttc act gac ggc gca acc 355Leu Gln Gly Pro Lys Ile Arg Leu Gly Arg Phe Thr Asp Gly Ala Thr70 75 80 85gtg tgg gaa aac ggc gag acc att cgg atc acc gtt gac gat gta gag 403Val Trp Glu Asn Gly Glu Thr Ile Arg Ile Thr Val Asp Asp Val Glu
90 95 100gga acg cac gat cgt gtg tcc acc acc tac aag aat ctg gca aaa gac 451Gly Thr His Asp Arg Val Ser Thr Thr Tyr Lys Asn Leu Ala Lys Asp
105 110 115gcg aag cca ggc gac cgc ctg ctc gtt gat gac ggc aag gtt ggc ctc 499Ala Lys Pro Gly Asp Arg Leu Leu Val Asp Asp Gly Lys Val Gly Leu
120 125 130gtc tgc gtt tcc gtc gaa ggt aac gac gtc atc tgt gag gtt gtt gag 547Val Cys Val Ser Val Glu Gly Asn Asp Val Ile Cys Glu Val Val Glu
135 140 145ggc gga cca gtc tcc aac aac aag ggt gtt tcc ctg cca ggt atg gat 595Gly Gly Pro Val Ser Asn Asn Lys Gly Val Ser Leu Pro Gly Met Asp150 155 160 165att tcc gta cct gca ctg tcc gaa aag gat atc cgt gac ctg cgc ttc 643Ile Ser Val Pro Ala Leu Ser Glu Lys Asp Ile Arg Asp Leu Arg Phe
170 175 180gcc ctg aag ctc ggc gtg gac ttt att gca ctg tcc ttc gta cgt tcc 691Ala Leu Lys Leu Gly Val Asp Phe Ile Ala Leu Ser Phe Val Arg Ser
185 190 195cca gca gat gct gaa ctc gtt cac aag atc atg gac gaa gaa ggt cgt 739Pro Ala Asp Ala Glu Leu Val His Lys Ile Met Asp Glu Glu Gly Arg
200 205 210cgt gtt cct gtg atc gcc aag ctg gaa aag cca gag gct gtc acc tcc 787Arg Val Pro Val Ile Ala Lys Leu Glu Lys Pro Glu Ala Val Thr Ser
215 220 225ctc gag cca atc gtg ttg gca ttc gac gcc gtc atg gtt gct cgt ggt 835Leu Glu Pro Ile Val Leu Ala Phe Asp Ala Val Met Val Ala Arg Gly230 235 240 245gac ctc ggc gtt gag gtt cct ctg gag gag gtt cca ctg gtt cag aag 883Asp Leu Gly Val Glu Val Pro Leu Glu Glu Val Pro Leu Val Gln Lys
250 255 260cgc gca atc cag att gcc cgt gag aac gca aag cca gtt atc gtg gca 931Arg Ala Ile Gln Ile Ala Arg Glu Asn Ala Lys Pro Val Ile Val Ala
265 270 275acc cag atg ctg gat tcc atg att gag aac tcc cgc cca acc cgt gcg 979Thr Gln Met Leu Asp Ser Met Ile Glu Asn Ser Arg Pro Thr Arg Ala
280 285 290gaa gct tct gac gtg gca aac gct gtg ctc gat ggc gca gat gct gtc 1027Glu Ala Ser Asp Val Ala Asn Ala Val Leu Asp Gly Ala Asp Ala Val
295 300 305atg ctt tct ggt gaa act tca gtg ggc aaa gat ccg cac aac gtt gtg 1075Met Leu Ser Gly Glu Thr Ser Val Gly Lys Asp Pro His Asn Val Val310 315 320 325cgc acc atg tct cgc att gtt cgc ttc gct gaa acc gac ggt cgc gtc 1123Arg Thr Met Ser Arg Ile Val Arg Phe Ala Glu Thr Asp Gly Arg Val
330 335 340cca gac ctg acc cac atc cct cgc act aag cgt ggc gtt att tcc tac 1171Pro Asp Leu Thr His Ile Pro Arg Thr Lys Arg Gly Val Ile Ser Tyr
345 350 355tct gca cgt gat atc gcc gag cgc ctc aac gct cgt gca ttg gtt gcg 1219Ser Ala Arg Asp Ile Ala Glu Arg Leu Asn Ala Arg Ala Leu Val Ala
360 365 370ttc acc acc tct ggt gat acc gca aag cgt gtg gct cgt ctg cac agc 1267Phe Thr Thr Ser Gly Asp Thr Ala Lys Arg Val Ala Arg Leu His Ser
375 380 385cac ctg cca ctg ctc gtg ttc act cca aat gag gca gtt cgc tct gag 1315His Leu Pro Leu Leu Val Phe Thr Pro Asn Glu Ala Val Arg Ser Glu390 395 400 405ctg gcg ctg acc tgg ggt gca acc acc ttc ctg tgt cca cct gtc agc 1363Leu Ala Leu Thr Trp Gly Ala Thr Thr Phe Leu Cys Pro Pro Val Ser
410 415 420gat acc gat gac atg atg cgc gaa gtc gac cgt gct ctt tta gca atg 1411Asp Thr Asp Asp Met Met Arg Glu Val Asp Arg Ala Leu Leu Ala Met
425 430 435cct gag tac aac aag ggt gac atg atg gtt gtt gtt gca ggt tcc cct 1459Pro Glu Tyr Asn Lys Gly Asp Met Met Val Val Val Ala Gly Ser Pro
440 445 450cct ggt gtt acc ggt aac acc aac atg att cac gtc cac ctt ctt ggt 1507Pro Gly Val Thr Gly Asn Thr Asn Met Ile His Val His Leu Leu Gly
455 460 465gac gac aca agg att gca aag ctc taatcgctta aatctttcaa aaa 1554Asp Asp Thr Arg Ile Ala Lys Leu470 475<210>74<211>477<212>PRT<213>谷氨酸棒杆菌<400>74Met Gly Val Asp Arg Arg Thr Lys Ile Val Cys Thr Leu Gly Pro Ala1 5 10 15Val Ala Ser Ala Asp Gly Ile Leu Arg Leu Val Glu Asp Gly Met Asp
20 25 30Val Ala Arg Leu Asn Phe Ser His Gly Asp His Pro Asp His Glu Gln
35 40 45Asn Tyr Lys Trp Val Arg Glu Ala Ala Glu Lys Thr Gly Arg Ala Val
50 55 60Gly Ile Leu Ala Asp Leu Gln Gly Pro Lys Ile Arg Leu Gly Arg Phe65 70 75 80Thr Asp Gly Ala Thr Val Trp Glu Asn Gly Glu Thr Ile Arg Ile Thr
85 90 95Val Asp Asp Val Glu Gly Thr His Asp Arg Val Ser Thr Thr Tyr Lys
100 105 110Asn Leu Ala Lys Asp Ala Lys Pro Gly Asp Arg Leu Leu Val Asp Asp
115 120 125Gly Lys Val Gly Leu Val Cys Val Ser Val Glu Gly Asn Asp Val Ile
130 135 140Cys Glu Val Val Glu Gly Gly Pro Val Ser Asn Asn Lys Gly Val Ser145 150 155 160Leu Pro Gly Met Asp Ile Ser Val Pro Ala Leu Ser Glu Lys Asp Ile
165 170 175Arg Asp Leu Arg Phe Ala Leu Lys Leu Gly Val Asp Phe Ile Ala Leu
180 185 190Ser Phe Val Arg Ser Pro Ala Asp Ala Glu Leu Val His Lys Ile Met
195 200 205Asp Glu Glu Gly Arg Arg Val Pro Val Ile Ala Lys Leu Glu Lys Pro
210 215 220Glu Ala Val Thr Ser Leu Glu Pro Ile Val Leu Ala Phe Asp Ala Val225 230 235 240Met Val Ala Arg Gly Asp Leu Gly Val Glu Val Pro Leu Glu Glu Val
245 250 255Pro Leu Val Gln Lys Arg Ala Ile Gln Ile Ala Arg Glu Asn Ala Lys
260 265 270Pro Val Ile Val Ala Thr Gln Met Leu Asp Ser Met Ile Glu Asn Ser
275 280 285Arg Pro Thr Arg Ala Glu Ala Ser Asp Val Ala Asn Ala Val Leu Asp
290 295 300Gly Ala Asp Ala Val Met Leu Ser Gly Glu Thr Ser Val Gly Lys Asp305 310 315 320Pro His Asn Val Val Arg Thr Met Ser Arg Ile Val Arg Phe Ala Glu
325 330 335Thr Asp Gly Arg Val Pro Asp Leu Thr His Ile Pro Arg Thr Lys Arg
340 345 350Gly Val Ile Ser Tyr Ser Ala Arg Asp Ile Ala Glu Arg Leu Asn Ala
355 360 365Arg Ala Leu Val Ala Phe Thr Thr Ser Gly Asp Thr Ala Lys Arg Val
370 375 380Ala Arg Leu His Ser His Leu Pro Leu Leu Val Phe Thr Pro Asn Glu385 390 395 400Ala Val Arg Ser Glu Leu Ala Leu Thr Trp Gly Ala Thr Thr Phe Leu
405 410 415Cys Pro Pro Val Ser Asp Thr Asp Asp Met Met Arg Glu Val Asp Arg
420 425 430Ala Leu Leu Ala Met Pro Glu Tyr Asn Lys Gly Asp Met Met Val Val
435 440 445Val Ala Gly Ser Pro Pro Gly Val Thr Gly Asn Thr Asn Met Ile His
450 455 460Val His Leu Leu Gly Asp Asp Thr Arg Ile Ala Lys Leu465 470 475<210>75<211>1980<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1957)<223>RXN02675<400>75aagtgtttca ttggaacact tgcgctgcca actttttggt ttacgggcac aatgaaactg 60ttggatggaa tttagagtgt ttgtagctta aggagctcaa atg aat gag ttt gac 115
Met Asn Glu Phe Asp
1 5cag gac att ctc cag gag atc aag act gaa ctc gac gag tta att cta 163Gln Asp Ile Leu Gln Glu Ile Lys Thr Glu Leu Asp Glu Leu Ile Leu
10 15 20gaa ctt gat gag gtg aca caa act cac agc gag gcc atc ggg cag gtc 211Glu Leu Asp Glu Val Thr Gln Thr His Ser Glu Ala Ile Gly Gln Val
25 30 35tcc cca acc cat tac gtt ggt gcc cgc aac ctc atg cat tac gcg cat 259Ser Pro Thr His Tyr Val Gly Ala Arg Asn Leu Met His Tyr Ala His
40 45 50ctt cgc acc aaa gac ctc cgt ggc ctg cag caa cgc ctc tcc tct gtg 307Leu Arg Thr Lys Asp Leu Arg Gly Leu Gln Gln Arg Leu Ser Ser Val
55 60 65gga gct acc cgc ttg act acc acc gaa cca gca gtg cag gcc cgc ctc 355Gly Ala Thr Arg Leu Thr Thr Thr Glu Pro Ala Val Gln Ala Arg Leu70 75 80 85aag gcc gcc cgc aat gtt atc gga gct ttc gca ggt gaa ggc cca ctt 403Lys Ala Ala Arg Asn Val Ile Gly Ala Phe Ala Gly Glu Gly Pro Leu
90 95 100tat cca ccc tca gat gtc gtc gat gcc ttc gaa gat gcc gat gag att 451Tyr Pro Pro Ser Asp Val Val Asp Ala Phe Glu Asp Ala Asp Glu Ile
105 110 115ctc gac gag cac gcc gaa att ctc ctt ggc gaa ccc cta ccg gat act 499Leu Asp Glu His Ala Glu Ile Leu Leu Gly Glu Pro Leu Pro Asp Thr
120 125 130cca tcc tgc atc atg gtc acc ctg ccc acc gaa gcc gcc acc gac att 547Pro Ser Cys Ile Met Val Thr Leu Pro Thr Glu Ala Ala Thr Asp Ile
135 140 145gaa ctt gtc cgt ggc ttc gcc aaa agc ggc atg aat cta gct cgc atc 595Glu Leu Val Arg Gly Phe Ala Lys Ser Gly Met Asn Leu Ala Arg Ile150 155 160 165aac tgt gca cac gac gat gaa acc gtc tgg aag cag atg atc gac aac 643Asn Cys Ala His Asp Asp Glu Thr Val Trp Lys Gln Met Ile Asp Asn
170 175 180gtc cac acc gtt gca gaa gaa gtt ggc cgg gaa atc cgc gtc agc atg 691Val His Thr Val Ala Glu Glu Val Gly Arg Glu Ile Arg Val Ser Met
185 190 195gac ctc gcc gga cca aaa gta cgc acc ggc gaa atc gcc cca ggc gca 739Asp Leu Ala Gly Pro Lys Val Arg Thr Gly Glu Ile Ala Pro Gly Ala
200 205 210gaa gta ggt cgc gca cga gta acc cgc gac gaa acc gga aaa gta ctg 787Glu Val Gly Arg Ala Arg Val Thr Arg Asp Glu Thr Gly Lys Val Leu
215 220 225acg ccc gca aaa ctg tgg atc acc gcc cac ggc tcc gaa cca gtc cca 835Thr Pro Ala Lys Leu Trp Ile Thr Ala His Gly Ser Glu Pro Val Pro230 235 240 245gcc ccc gaa agc ctg ccc ggt cgc ccc gct ctg ccg att gaa gtc acc 883Ala Pro Glu Ser Leu Pro Gly Arg Pro Ala Leu Pro Ile Glu Val Thr
250 255 260cca gaa tgg ttc gac aaa cta gaa atc ggc agc gtc atc aac gtc cca 931Pro Glu Trp Phe Asp Lys Leu Glu Ile Gly Ser Val Ile Asn Val Pro
265 270 275gac acc cgc gga tcc cgc cga gca ttc acc gtg acc agg gtt ttt gat 979Asp Thr Arg Gly Ser Arg Arg Ala Phe Thr Val Thr Arg Val Phe Asp
280 285 290ggc gcg gtc ctc gcc gaa ggc cca caa aaa gcc tac atc tcc aac ggc 1027Gly Ala Val Leu Ala Glu Gly Pro Gln Lys Ala Tyr Ile Ser Asn Gly
295 300 305acc ctc ctg gaa cac aac tac gac cgc tcc cgg gtc tac ggc atc ccc 1075Thr Leu Leu Glu His Asn Tyr Asp Arg Ser Arg Val Tyr Gly Ile Pro310 315 320 325gcc gta gtt cag cgc atc aac ctc aaa gtc ggc gac cgc ctc atc ctt 1123Ala Val Val Gln Arg Ile Asn Leu Lys Val Gly Asp Arg Leu Ile Leu
330 335 340acc gac gaa gaa ctc acc tac gat cca tcc ctc gga tcc ggc cgc aca 1171Thr Asp Glu Glu Leu Thr Tyr Asp Pro Ser Leu Gly Ser Gly Arg Thr
345 350 355cca cgc atc agc tgc acc ctt cca caa gca gtc gat gca att aaa gtc 1219Pro Arg Ile Ser Cys Thr Leu Pro Gln Ala Val Asp Ala Ile Lys Val
360 365 370ggg cac cgc gtg ctt ttc gac gac gga gcc atc gcc gca gtc tgc atc 1267Gly His Arg Val Leu Phe Asp Asp Gly Ala Ile Ala Ala Val Cys Ile
375 380 385gac aag acc tcc act gcc gac ggc cac aac gac gta gaa ttg gaa gtc 1315Asp Lys Thr Ser Thr Ala Asp Gly His Asn Asp Val Glu Leu Glu Val390 395 400 405acc cac gcc cgc cea caa ggc gta aac ctg gcc gca tac aag gga atc 1363Thr His Ala Arg Pro Gln Gly Val Asn Leu Ala Ala Tyr Lys Gly Ile
410 415 420aac ctc cca gac tcc gaa ctt cca ctc cca agc ctc act gaa gaa gac 1411Asn Leu Pro Asp Ser Glu Leu Pro Leu Pro Ser Leu Thr Glu Glu Asp
425 430 435ctc caa cac ctg cgc ttt gtc gtc aaa tac gcc gac atc gca gcc atc 1459Leu Gln His Leu Arg Phe Val Val Lys Tyr Ala Asp Ile Ala Ala Ile
440 445 450tcc ttc atc cga aac gtc gcc gac gtg gaa tac ctc ctc caa gca ctc 1507Ser Phe Ile Arg Asn Val Ala Asp Val Glu Tyr Leu Leu Gln Ala Leu
455 460 465gcc gac atc gga gat cca gta gcc gtc gaa cgc ctt ggc ctc gtc ctt 1555Ala Asp Ile Gly Asp Pro Val Ala Val Glu Arg Leu Gly Leu Val Leu470 475 480 485aaa atc gag acc atc cca ggc tac gaa ggc ctc gcc caa atc ctc ctg 1603Lys Ile Glu Thr Ile Pro Gly Tyr Glu Gly Leu Ala Gln Ile Leu Leu
490 495 500acc ggc atg cgc cac gaa aac ttc ggc atc atg atc gcc cgc gga gac 1651Thr Gly Met Arg His Glu Asn Phe Gly Ile Met Ile Ala Arg Gly Asp
505 510 515ctc gcc gtc gaa ctc ggc ttc gac cgc atg gca gaa gtc ccc caa ctg 1699Leu Ala Val Glu Leu Gly Phe Asp Arg Met Ala Glu Val Pro Gln Leu
520 525 530atc atg gcc ctt gcc gaa gcc gcc cac gtc cca acc atc ttg gcc acc 1747Ile Met Ala Leu Ala Glu Ala Ala His Val Pro Thr Ile Leu Ala Thr
535 540 545caa gtc ctg gaa aac atg gcc aaa aac gga ctc cca tct cgc gca gaa 1795Gln Val Leu Glu Asn Met Ala Lys Asn Gly Leu Pro Ser Arg Ala Glu550 555 560 565atc acc gac gca gca atg gca ctt cgc gct gaa tgc gtc atg ctg aac 1843Ile Thr Asp Ala Ala Met Ala Leu Arg Ala Glu Cys Val Met Leu Asn
570 575 580aag gga cca cac atc aac gac gcc atc aag gtc ctc acc gaa atg agc 1891Lys Gly Pro His Ile Asn Asp Ala Ile Lys Val Leu Thr Glu Met Ser
585 590 595cgc aaa ctt ggt gca tcc caa cga aag agt agg ctg ctg ctg cgc aag 1939Arg Lys Leu Gly Ala Ser Gln Arg Lys Ser Arg Leu Leu Leu Arg Lys
600 605 610gtg aag agc tgg gaa gag taactcacaa aggcgattgg cgt 1980Val Lys Ser Trp Glu Glu
615<210>76<211>619<212>PRT<213>谷氨酸棒杆菌<400>76Met Asn Glu Phe Asp Gln Asp Ile Leu Gln Glu Ile Lys Thr Glu Leu1 5 10 15Asp Glu Leu Ile Leu Glu Leu Asp Glu Val Thr Gln Thr His Ser Glu
20 25 30Ala Ile Gly Gln Val Ser Pro Thr His Tyr Val Gly Ala Arg Asn Leu
35 40 45Met His Tyr Ala His Leu Arg Thr Lys Asp Leu Arg Gly Leu Gln Gln
50 55 60Arg Leu Ser Ser Val Gly Ala Thr Arg Leu Thr Thr Thr Glu Pro Ala65 70 75 80Val Gln Ala Arg Leu Lys Ala Ala Arg Asn Val Ile Gly Ala Phe Ala
85 90 95Gly Glu Gly Pro Leu Tyr Pro Pro Ser Asp Val Val Asp Ala Phe Glu
100 105 110Asp Ala Asp Glu Ile Leu Asp Glu His Ala Glu Ile Leu Leu Gly Glu
115 120 125Pro Leu Pro Asp Thr Pro Ser Cys Ile Met Val Thr Leu Pro Thr Glu
130 135 140Ala Ala Thr Asp Ile Glu Leu Val Arg Gly Phe Ala Lys Ser Gly Met145 150 155 160Asn Leu Ala Arg Ile Asn Cys Ala His Asp Asp Glu Thr Val Trp Lys
165 170 175Gln Met Ile Asp Asn Val His Thr Val Ala Glu Glu Val Gly Arg Glu
180 185 190Ile Arg Val Ser Met Asp Leu Ala Gly Pro Lys Val Arg Thr Gly Glu
195 200 205Ile Ala Pro Gly Ala Glu Val Gly Arg Ala Arg Val Thr Arg Asp Glu
210 215 220Thr Gly Lys Val Leu Thr Pro Ala Lys Leu Trp Ile Thr Ala His Gly225 230 235 240Ser Glu Pro Val Pro Ala Pro Glu Ser Leu Pro Gly Arg Pro Ala Leu
245 250 255Pro Ile Glu Val Thr Pro Glu Trp Phe Asp Lys Leu Glu Ile Gly Ser
260 265 270Val Ile Asn Val Pro Asp Thr Arg Gly Ser Arg Arg Ala Phe Thr Val
275 280 285Thr Arg Val Phe Asp Gly Ala Val Leu Ala Glu Gly Pro Gln Lys Ala
290 295 300Tyr Ile Ser Asn Gly Thr Leu Leu Glu His Asn Tyr Asp Arg Ser Arg305 310 315 320Val Tyr Gly Ile Pro Ala Val Val Gln Arg Ile Asn Leu Lys Val Gly
325 330 335Asp Arg Leu Ile Leu Thr Asp Glu Glu Leu Thr Tyr Asp Pro Ser Leu
340 345 350Gly Ser Gly Arg Thr Pro Arg Ile Ser Cys Thr Leu Pro Gln Ala Val
355 360 365Asp Ala Ile Lys Val Gly His Arg Val Leu Phe Asp Asp Gly Ala Ile
370 375 380Ala Ala Val Cys Ile Asp Lys Thr Ser Thr Ala Asp Gly His Asn Asp385 390 395 400Val Glu Leu Glu Val Thr His Ala Arg Pro Gln Gly Val Asn Leu Ala
405 410 415Ala Tyr Lys Gly Ile Asn Leu Pro Asp Ser Glu Leu Pro Leu Pro Ser
420 425 430Leu Thr Glu Glu Asp Leu Gln His Leu Arg Phe Val Val Lys Tyr Ala
435 440 445Asp Ile Ala Ala Ile Ser Phe Ile Arg Asn Val Ala Asp Val Glu Tyr
450 455 460Leu Leu Gln Ala Leu Ala Asp Ile Gly Asp Pro Val Ala Val Glu Arg465 470 475 480Leu Gly Leu Val Leu Lys Ile Glu Thr Ile Pro Gly Tyr Glu Gly Leu
485 490 495Ala Gln Ile Leu Leu Thr Gly Met Arg His Glu Asn Phe Gly Ile Met
500 505 510Ile Ala Arg Gly Asp Leu Ala Val Glu Leu Gly Phe Asp Arg Met Ala
515 520 525Glu Val Pro Gln Leu Ile Met Ala Leu Ala Glu Ala Ala His Val Pro
530 535 540Thr Ile Leu Ala Thr Gln Val Leu Glu Asn Met Ala Lys Asn Gly Leu545 550 555 560Pro Ser Arg Ala Glu Ile Thr Asp Ala Ala Met Ala Leu Arg Ala Glu
565 570 575Cys Val Met Leu Asn Lys Gly Pro His Ile Asn Asp Ala Ile Lys Val
580 585 590Leu Thr Glu Met Ser Arg Lys Leu Gly Ala Ser Gln Arg Lys Ser Arg
595 600 605Leu Leu Leu Arg Lys Val Lys Ser Trp Glu Glu
610 615<210>77<211>386<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(363)<223>FRXA02675<400>77atc ctc atg acc ggc atg cgc cac gaa aac ttc ggc atc atg atc gcc 48Ile Leu Met Thr Gly Met Arg His Glu Asn Phe Gly Ile Met Ile Ala1 5 10 15cgc gga gac ctc gcc gtc gaa ctc ggc ttc gac cgc atg gca gaa gtc 96Arg Gly Asp Leu Ala Val Glu Leu Gly Phe Asp Arg Met Ala Glu Val
20 25 30ccc caa ctg atc atg gcc ctt gca gaa gcc gcc cac gtc cca acc atc 144Pro Gln Leu Ile Met Ala Leu Ala Glu Ala Ala His Val Pro Thr Ile
35 40 45ttg gcc acc caa gtc ctg gaa aac atg gcc aaa aac gga ctc cca tct 192Leu Ala Thr Gln Val Leu Glu Asn Met Ala Lys Asn Gly Leu Pro Ser
50 55 60cgc gca gaa atc acc gac gca gca atg gca ctt cgc gct gaa tgc gtc 240Arg Ala Glu Ile Thr Asp Ala Ala Met Ala Leu Arg Ala Glu Cys Val65 70 75 80atg ctg aac aag gga cca cac atc aac gac gcc atc aag gtc ctc acc 288Met Leu Asn Lys Gly Pro His Ile Asn Asp Ala Ile Lys Val Leu Thr
85 90 95gaa atg agc cgc aaa ctt ggt gca tcc caa cga aag agt agg ctg ctg 336Glu Met Ser Arg Lys Leu Gly Ala Ser Gln Arg Lys Ser Arg Leu Leu
100 105 110ctg cgc aag gtg aag agc tgg gaa gag taactcacaa aggcgattgg 383Leu Arg Lys Val Lys Ser Trp Glu Glu
115 120cgt 386<210>78<211>121<212>PRT<213>谷氨酸棒杆菌<400>78Ile Leu Met Thr Gly Met Arg His Glu Asn Phe Gly Ile Met Ile Ala1 5 10 15Arg Gly Asp Leu Ala Val Glu Leu Gly Phe Asp Arg Met Ala Glu Val
20 25 30Pro Gln Leu Ile Met Ala Leu Ala Glu Ala Ala His Val Pro Thr Ile
35 40 45Leu Ala Thr Gln Val Leu Glu Asn Met Ala Lys Asn Gly Leu Pro Ser
50 55 60Arg Ala Glu Ile Thr Asp Ala Ala Met Ala Leu Arg Ala Glu Cys Val65 70 75 80Met Leu Asn Lys Gly Pro His Ile Asn Asp Ala Ile Lys Val Leu Thr
85 90 95Glu Met Ser Arg Lys Leu Gly Ala Ser Gln Arg Lys Ser Arg Leu Leu
100 105 110Leu Arg Lys Val Lys Ser Trp Glu Glu
115 120<210>79<211>1522<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1522)<223>FRXA02695<400>79aagtgtttca ttggaacact tgcgctgcca actttttggt ttacgggcac aatgaaactg 60ttggatggaa tttagagtgt ttgtagctta aggagctcaa atg aat gag ttt gac 115
Met Asn Glu Phe Asp
l 5cag gac att ctc cag gag atc aag act gaa ctc gac gag tta att cta 163Gln Asp Ile Leu Gln Glu Ile Lys Thr Glu Leu Asp Glu Leu Ile Leu
10 15 20gaa ctt gat gag gtg aca caa act cac agc gag gcc atc ggg cag gtc 211Glu Leu Asp Glu Val Thr Gln Thr His Ser Glu Ala Ile Gly Gln Val
25 30 35tcc cca acc cat tac gtt ggt gcc cgc aac ctc atg cat tac gcg cat 259Ser Pro Thr His Tyr Val Gly Ala Arg Asn Leu Met His Tyr Ala His
40 45 50ctt cgc acc aaa gac ctc cgt ggc ctg cag caa cgc ctc tcc tct gtg 307Leu Arg Thr Lys Asp Leu Arg Gly Leu Gln Gln Arg Leu Ser Ser Val
55 60 65gga gct acc cgc ttg act acc acc gaa cca gca gtg cag gcc cgc ctc 355Gly Ala Thr Arg Leu Thr Thr Thr Glu Pro Ala Val Gln Ala Arg Leu70 75 80 85aag gcc gcc cgc aat gtt atc gga gct ttc gca ggt gaa ggc cca ctt 403Lys Ala Ala Arg Asn Val Ile Gly Ala Phe Ala Gly Glu Gly Pro Leu
90 95 100tat cca ccc tca gat gtc gtc gat gcc ttc gaa gat gcc gat gag att 451Tyr Pro Pro Ser Asp Val Val Asp Ala Phe Glu Asp Ala Asp Glu Ile
105 110 115ctc gac gag cac gcc gaa att ctc ctt ggc gaa ccc cta ccg gat act 499Leu Asp Glu His Ala Glu Ile Leu Leu Gly Glu Pro Leu Pro Asp Thr
120 125 130cca tcc tgc atc atg gtc acc ctg ccc acc gaa gcc gcc acc gac att 547Pro Ser Cys Ile Met Val Thr Leu Pro Thr Glu Ala Ala Thr Asp Ile
135 140 145gaa ctt gtc cgt ggc ttc gcc aaa agc ggc atg aat cta gct cgc atc 595Glu Leu Val Arg Gly Phe Ala Lys Ser Gly Met Asn Leu Ala Arg Ile150 155 160 165aac tgt gca cac gac gat gaa acc gtc tgg aag cag atg atc gac aac 643Asn Cys Ala His Asp Asp Glu Thr Val Trp Lys Gln Met Ile Asp Asn
170 175 180gtc cac acc gtt gca gaa gaa gtt ggc cgg gaa atc cgc gtc agc atg 691Val His Thr Val Ala Glu Glu Val Gly Arg Glu Ile Arg Val Ser Met
185 190 195gac ctc gcc gga cca aaa gta cgc acc ggc gaa atc gcc cca ggc gca 739Asp Leu Ala Gly Pro Lys Val Arg Thr Gly Glu Ile Ala Pro Gly Ala
200 205 210gaa gta ggt cgc gca cga gta acc cgc gac gaa acc gga aaa gta ctg 787Glu Val Gly Arg Ala Arg Val Thr Arg Asp Glu Thr Gly Lys Val Leu
215 220 225acg ccc gca aaa ctg tgg atc acc gcc cac ggc tcc gaa cca gtc cca 835Thr Pro Ala Lys Leu Trp Ile Thr Ala His Gly Ser Glu Pro Val Pro230 235 240 245gcc ccc gaa agc ctg ccc ggt cgc ccc gct ctg ccg att gaa gtc acc 883Ala Pro Glu Ser Leu Pro Gly Arg Pro Ala Leu Pro Ile Glu Val Thr
250 255 260cca gaa tgg ttc gac aaa cta gaa atc ggc agc gtc atc aac gtc cca 931Pro Glu Trp Phe Asp Lys Leu Glu Ile Gly Ser Val Ile Asn Val Pro
265 270 275gac acc cgc gga tcc cgc cga gca ttc acc gtg acc agg gtt ttt gat 979Asp Thr Arg Gly Ser Arg Arg Ala Phe Thr Val Thr Arg Val Phe Asp
280 285 290ggc gcg gtc ctc gcc gaa ggc cca caa aaa gcc tac atc tcc aac ggc 1027Gly Ala Val Leu Ala Glu Gly Pro Gln Lys Ala Tyr Ile Ser Asn Gly
295 300 305acc ctc ctg gaa cac aac tac gac cgc tcc cgg gtc tac ggc atc ccc 1075Thr Leu Leu Glu His Asn Tyr Asp Arg Ser Arg Val Tyr Gly Ile Pro310 315 320 325gcc gta gtt cag cgc atc aac ctc aaa gtc ggc gac cgc ctc atc ctt 1123Ala Val Val Gln Arg Ile Asn Leu Lys Val Gly Asp Arg Leu Ile Leu
330 335 340acc gac gaa gaa ctc acc tac gat cca tcc ctc gga tcc ggc cgc aca 1171Thr Asp Glu Glu Leu Thr Tyr Asp Pro Ser Leu Gly Ser Gly Arg Thr
345 350 355cca cgc atc agc tgc acc ctt cca caa gca gtc gat gca att aaa gtc 1219Pro Arg Ile Ser Cys Thr Leu Pro Gln Ala Val Asp Ala Ile Lys Val
360 365 370ggg cac cgc gtg ctt ttc gac gac gga gcc atc gcc gca gtc tgc atc 1267Gly His Arg Val Leu Phe Asp Asp Gly Ala Ile Ala Ala Val Cys Ile
375 380 385gac aag acc tcc act gcc gac ggc cac aac gac gta gaa ttg gaa gtc 1315Asp Lys Thr Ser Thr Ala Asp Gly His Asn Asp Val Glu Leu Glu Val390 395 400 405acc cac gcc cgc cca caa ggc gta aac ctg gcc gca tac aag gga atc 1363Thr His Ala Arg Pro Gln Gly Val Asn Leu Ala Ala Tyr Lys Gly Ile
410 415 420aac ctc cca gac tcc gaa ctt cca ctc cca agc ctc act gaa gaa gac 1411Asn Leu Pro Asp Ser Glu Leu Pro Leu Pro Ser Leu Thr Glu Glu Asp
425 430 435ctc caa cac ctg cgc ttt gtc gtc aaa tac gcc gac atc gca gcc atc 1459Leu Gln His Leu Arg Phe Val Val Lys Tyr Ala Asp Ile Ala Ala Ile
440 445 450tcc ttc atc cga aac gtc gcc gac gtg gaa tac ctc ctc caa gca ctc 1507Ser Phe Ile Arg Asn Val Ala Asp Val Glu Tyr Leu Leu Gln Ala Leu
455 460 465gcc gac atc gga gat 1522Ala Asp Ile Gly Asp470<210>80<211>474<212>PRT<213>谷氨酸棒杆菌<400>80Met Asn Glu Phe Asp Gln Asp Ile Leu Gln Glu Ile Lys Thr Glu Leu1 5 10 15Asp Glu Leu Ile Leu Glu Leu Asp Glu Val Thr Gln Thr His Ser Glu
20 25 30Ala Ile Gly Gln Val Ser Pro Thr His Tyr Val Gly Ala Arg Asn Leu
35 40 45Met His Tyr Ala His Leu Arg Thr Lys Asp Leu Arg Gly Leu Gln Gln
50 55 60Arg Leu Ser Ser Val Gly Ala Thr Arg Leu Thr Thr Thr Glu Pro Ala65 70 75 80Val Gln Ala Arg Leu Lys Ala Ala Arg Asn Val Ile Gly Ala Phe Ala
85 90 95Gly Glu Gly Pro Leu Tyr Pro Pro Ser Asp Val Val Asp Ala Phe Glu
100 105 110Asp Ala Asp Glu Ile Leu Asp Glu His Ala Glu Ile Leu Leu Gly Glu
115 120 125Pro Leu Pro Asp Thr Pro Ser Cys Ile Met Val Thr Leu Pro Thr Glu
130 135 140Ala Ala Thr Asp Ile Glu Leu Val Arg Gly Phe Ala Lys Ser Gly Met145 150 155 160Asn Leu Ala Arg Ile Asn Cys Ala His Asp Asp Glu Thr Val Trp Lys
165 170 175Gln Met Ile Asp Asn Val His Thr Val Ala Glu Glu Val Gly Arg Glu
180 185 190Ile Arg Val Ser Met Asp Leu Ala Gly Pro Lys Val Arg Thr Gly Glu
195 200 205Ile Ala Pro Gly Ala Glu Val Gly Arg Ala Arg Val Thr Arg Asp Glu
210 215 220Thr Gly Lys Val Leu Thr Pro Ala Lys Leu Trp Ile Thr Ala His Gly225 230 235 240Ser Glu Pro Val Pro Ala Pro Glu Ser Leu Pro Gly Arg Pro Ala Leu
245 250 255Pro Ile Glu Val Thr Pro Glu Trp Phe Asp Lys Leu Glu Ile Gly Ser
260 265 270Val Ile Asn Val Pro Asp Thr Arg Gly Ser Arg Arg Ala Phe Thr Val
275 280 285Thr Arg Val Phe Asp Gly Ala Val Leu Ala Glu Gly Pro Gln Lys Ala
290 295 300Tyr Ile Ser Asn Gly Thr Leu Leu Glu His Asn Tyr Asp Arg Ser Arg305 310 315 320Val Tyr Gly Ile Pro Ala Val Val Gln Arg Ile Asn Leu Lys Val Gly
325 330 335Asp Arg Leu Ile Leu Thr Asp Glu Glu Leu Thr Tyr Asp Pro Ser Leu
340 345 350Gly Ser Gly Arg Thr Pro Arg Ile Ser Cys Thr Leu Pro Gln Ala Val
355 360 365Asp Ala Ile Lys Val Gly His Arg Val Leu Phe Asp Asp Gly Ala Ile
370 375 380Ala Ala Val Cys Ile Asp Lys Thr Ser Thr Ala Asp Gly His Asn Asp385 390 395 400Val Glu Leu Glu Val Thr His Ala Arg Pro Gln Gly Val Asn Leu Ala
405 410 415Ala Tyr Lys Gly Ile Asn Leu Pro Asp Ser Glu Leu Pro Leu Pro Ser
420 425 430Leu Thr Glu Glu Asp Leu Gln His Leu Arg Phe Val Val Lys Tyr Ala
435 440 445Asp Ile Ala Ala Ile Ser Phe Ile Arg Asn Val Ala Asp Val Glu Tyr
450 455 460Leu Leu Gln Ala Leu Ala Asp Ile Gly Asp465 470<210>81<211>2022<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1999)<223>RXA00682<400>81ataggcacct tcgatttcag ctcaatcacc gtcgcaatga ccggcacgaa gtaaaaccac 60cgcatctttt cgtcgaaaag catctaaaag gagtttgacc atg gct aat aaa tct 115
Met Ala Asn Lys Ser
1 5ttc ccc aag ccc tcc gat ctt cca gtg ccc aag ggc gct gaa ggt tgg 163Phe Pro Lys Pro Ser Asp Leu Pro Val Pro Lys Gly Ala Glu Gly Trp
10 15 20gaa gat ctg tac ccg tac tac ctc gtt ttc caa gac aag ctc atg gat 211Glu Asp Leu Tyr Pro Tyr Tyr Leu Val Phe Gln Asp Lys Leu Met Asp
25 30 35caa gag aat gag aaa ttc tgg ttc tgc gat tca cag cac tgg cca act 259Gln Glu Asn Glu Lys Phe Trp Phe Cys Asp Ser Gln His Trp Pro Thr
40 45 50gtg ttc aag cct ttt gaa act atc ggt ggt gaa ttc gct gta aag tgc 307Val Phe Lys Pro Phe Glu Thr Ile Gly Gly Glu Phe Ala Val Lys Cys
55 60 65ctc ggc caa tac aac gct cgg cat ttg atg atc ccg aat gcc aat ggc 355Leu Gly Gln Tyr Asn Ala Arg His Leu Met Ile Pro Asn Ala Asn Gly70 75 80 85atc gag ttc cgc gtg cat ctg gga tac ctc tat atg tcc cct att cca 403Ile Glu Phe Arg Val His Leu Gly Tyr Leu Tyr Met Ser Pro Ile Pro
90 95 100gtg cct gaa gat cag att gcg gaa cgc gtc ccc atg ttc cag gaa cgc 451Val Pro Glu Asp Gln Ile Ala Glu Arg Val Pro Met Phe Gln Glu Arg
105 110 115atc acg cac tac ttc caa aac tgg gag cca atg ctg gca aat tgg aag 499Ile Thr His Tyr Phe Gln Asn Trp Glu Pro Met Leu Ala Asn Trp Lys
120 125 130gag cga gta tta gga acc atc aat gag ctg gaa tct cta gaa ttc aag 547Glu Arg Val Leu Gly Thr Ile Asn Glu Leu Glu Ser Leu Glu Phe Lys
135 140 145cca ctg cct gac tac gtg cct atc gat gat att gtc tcc gga aaa gcc 595Pro Leu Pro Asp Tyr Val Pro Ile Asp Asp Ile Val Ser Gly Lys Ala150 155 160 165aaa gac ggc acc gaa gta ctc atg gaa aac ttc gat cgg ctc att cag 643Lys Asp Gly Thr Glu Val Leu Met Glu Asn Phe Asp Arg Leu Ile Gln
170 175 180ctc gcc tac caa aac tgg caa tac cac ttt gag ttc ctc aac ttg ggt 691Leu Ala Tyr Gln Asn Trp Gln Tyr His Phe Glu Phe Leu Asn Leu Gly
185 190 195tac atc gct tac cta gat ttc ttc aat ttc tgc aag gaa gtc ttc cca 739Tyr Ile Ala Tyr Leu Asp Phe Phe Asn Phe Cys Lys Glu Val Phe Pro
200 205 210gat atc cct gat caa tca att tcg atg atg gtt cag ggc gtg gat atg 787Asp Ile Pro Asp Gln Ser Ile Ser Met Met Val Gln Gly Val Asp Met
215 220 225gag ctg ttc cgc ccc gat gat gaa cta aag att ctg gca cag cta gcg 835Glu Leu Phe Arg Pro Asp Asp Glu Leu Lys Ile Leu Ala Gln Leu Ala230 235 240 245gtc gac ctt ggc ctg caa act cac ttt gcc aac ccg gat gat ccg caa 883Val Asp Leu Gly Leu Gln Thr His Phe Ala Asn Pro Asp Asp Pro Gln
250 255 260gct acc ttg gct gct atc gca aag gca gaa ggc ggc gcg aca tgg ata 931Ala Thr Leu Ala Ala Ile Ala Lys Ala Glu Gly Gly Ala Thr Trp Ile
265 270 275gcg cgc tgg gaa gaa gca caa gat ccg tgg ttc aac ttc acc gtc ggt 979Ala Arg Trp Glu Glu Ala Gln Asp Pro Trp Phe Asn Phe Thr Val Gly
280 285 290aat ggc ttc tac ggt cac gat aaa tac tgg atc gag cac ctg gaa ctt 1027Asn Gly Phe Tyr Gly His Asp Lys Tyr Trp Ile Glu His Leu Glu Leu
295 300 305cca ctg ggg tac atc gcg gat tac atc cgc cgc cta gat gaa ggc caa 1075Pro Leu Gly Tyr Ile Ala Asp Tyr Ile Arg Arg Leu Asp Glu Gly Gln310 315 320 325acc atc tcc cgc ccg aaa gat gaa ctc atc gca gaa aag gaa cgc gtg 1123Thr Ile Ser Arg Pro Lys Asp Glu Leu Ile Ala Glu Lys Glu Arg Val
330 335 340gtg gaa gaa tac cgc gac ctt ttg gat gga gaa caa ctc gcg cag ttt 1171Val Glu Glu Tyr Arg Asp Leu Leu Asp Gly Glu Gln Leu Ala Gln Phe
345 350 355gat gct aaa tgc ggc ctc gct gct act gca tac ccc tat gtg gaa aac 1219Asp Ala Lys Cys Gly Leu Ala Ala Thr Ala Tyr Pro Tyr Val Glu Asn
360 365 370cat aac ttc tac atc gag cac tgg acc atg tca gta ttt tgg cgc aaa 1267His Asn Phe Tyr Ile Glu His Trp Thr Met Ser Val Phe Trp Arg Lys
375 380 385gta cgc gaa ctt tcc cgc act ctc cag ggc tac ggt ttc tgg gag aac 1315Val Arg Glu Leu Ser Arg Thr Leu Gln Gly Tyr Gly Phe Trp Glu Asn390 395 400 405gag gat gac atg ttg tac ctc aac cgc act gaa gtc cgc gat gtc ctc 1363Glu Asp Asp Met Leu Tyr Leu Asn Arg Thr Glu Val Arg Asp Val Leu
410 415 420ttc gac ctg gct act gcg tgg ggt gtc ggc gca ccc ggt ggt cca att 1411Phe Asp Leu Ala Thr Ala Trp Gly Val Gly Ala Pro Gly Gly Pro Ile
425 430 435ggc acg atc att tgg ccg gaa gaa att gag cga aga aaa gca att gtc 1459Gly Thr Ile Ile Trp Pro Glu Glu Ile Glu Arg Arg Lys Ala Ile Val
440 445 450acc gct ttg aaa act gcc cga cca gcg cca gct ctt aac act cct cca 1507Thr Ala Leu Lys Thr Ala Arg Pro Ala Pro Ala Leu Asn Thr Pro Pro
455 460 465gag tcc atc acc gaa cct ttc acc cgc atg ctc tgg gga atc acc acc 1555Glu Ser Ile Thr Glu Pro Phe Thr Arg Met Leu Trp Gly Ile Thr Thr470 475 480 485gaa cag gtg caa tca tgg ttg ggc aat gac gag gat gcc gaa gaa gga 1603Glu Gln Val Gln Ser Trp Leu Gly Asn Asp Glu Asp Ala Glu Glu Gly
490 495 500acc ctt aaa ggc atg gct gca tcc cct ggt gtg gtg gaa ggc tac gct 165lThr Leu Lys Gly Met Ala Ala Ser Pro Gly Val Val Glu Gly Tyr Ala
505 510 515cga gta att ctc agc gca gat gac ctt tca gaa atc cag cag gat gaa 1699Arg Val Ile Leu Ser Ala Asp Asp Leu Ser Glu Ile Gln Gln Asp Glu
520 525 530atc ctc gtt gcc cct gta aca gca cct tct tgg ggc cca atc ttt ggc 1747Ile Leu Val Ala Pro Val Thr Ala Pro Ser Trp Gly Pro Ile Phe Gly
535 540 545aaa atc aag gca aca gtc act gat att ggt ggc atg atg agc cat gct 1795Lys Ile Lys Ala Thr Val Thr Asp Ile Gly Gly Met Met Ser His Ala550 555 560 565gcg atc gtg tgc cgc gaa tac ggc ttg ccg gct gtt act gga act ggc 1843Ala Ile Val Cys Arg Glu Tyr Gly Leu Pro Ala Val Thr Gly Thr Gly
570 575 580gct gca tcc acc acc atc aaa acc ggc gat tac ctc aag gtc gat gga 1891Ala Ala Ser Thr Thr Ile Lys Thr Gly Asp Tyr Leu Lys Val Asp Gly
585 590 595acc aag ggc aag gtt gtc att gtt gat cca gat gcg cca cgc atc gaa 1939Thr Lys Gly Lys Val Val Ile Val Asp Pro Asp Ala Pro Arg Ile Glu
600 605 610gga ccc ggc gcg cac agc cat gcg cac tca gta gca gca cat ggg gtg 1987Gly Pro Gly Ala His Ser His Ala His Ser Val Ala Ala His Gly Val
615 620 625gat aca cat gcc tagtccacgc actgttctta tca 2022Asp Thr His Ala630<210>82<211>633<212>PRT<213>谷氨酸棒杆菌<400>82Met Ala Asn Lys Ser Phe Pro Lys Pro Ser Asp Leu Pro Val Pro Lys1 5 10 15Gly Ala Glu Gly Trp Glu Asp Leu Tyr Pro Tyr Tyr Leu Val Phe Gln
20 25 30Asp Lys Leu Met Asp Gln Glu Asn Glu Lys Phe Trp Phe Cys Asp Ser
35 40 45Gln His Trp Pro Thr Val Phe Lys Pro Phe Glu Thr Ile Gly Gly Glu
50 55 60Phe Ala Val Lys Cys Leu Gly Gln Tyr Asn Ala Arg His Leu Met Ile65 70 75 80Pro Asn Ala Asn Gly Ile Glu Phe Arg Val His Leu Gly Tyr Leu Tyr
85 90 95Met Ser Pro Ile Pro Val Pro Glu Asp Gln Ile Ala Glu Arg Val Pro
100 105 110Met Phe Gln Glu Arg Ile Thr His Tyr Phe Gln Asn Trp Glu Pro Met
115 120 125Leu Ala Asn Trp Lys Glu Arg Val Leu Gly Thr Ile Asn Glu Leu Glu
130 135 140Ser Leu Glu Phe Lys Pro Leu Pro Asp Tyr Val Pro Ile Asp Asp Ile145 150 155 160Val Ser Gly Lys Ala Lys Asp Gly Thr Glu Val Leu Met Glu Asn Phe
165 170 175Asp Arg Leu Ile Gln Leu Ala Tyr Gln Asn Trp Gln Tyr His Phe Glu
180 185 190Phe Leu Asn Leu Gly Tyr Ile Ala Tyr Leu Asp Phe Phe Asn Phe Cys
195 200 205Lys Glu Val Phe Pro Asp Ile Pro Asp Gln Ser Ile Ser Met Met Val
210 215 220Gln Gly Val Asp Met Glu Leu Phe Arg Pro Asp Asp Glu Leu Lys Ile225 230 235 240Leu Ala Gln Leu Ala Val Asp Leu Gly Leu Gln Thr His Phe Ala Asn
245 250 255Pro Asp Asp Pro Gln Ala Thr Leu Ala Ala Ile Ala Lys Ala Glu Gly
260 265 270Gly Ala Thr Trp Ile Ala Arg Trp Glu Glu Ala Gln Asp Pro Trp Phe
275 280 285Asn Phe Thr Val Gly Asn Gly Phe Tyr Gly His Asp Lys Tyr Trp Ile
290 295 300Glu His Leu Glu Leu Pro Leu Gly Tyr Ile Ala Asp Tyr Ile Arg Arg305 310 315 320Leu Asp Glu Gly Gln Thr Ile Ser Arg Pro Lys Asp Glu Leu Ile Ala
325 330 335Glu Lys Glu Arg Val Val Glu Glu Tyr Arg Asp Leu Leu Asp Gly Glu
340 345 350Gln Leu Ala Gln Phe Asp Ala Lys Cys Gly Leu Ala Ala Thr Ala Tyr
355 360 365Pro Tyr Val Glu Asn His Asn Phe Tyr Ile Glu His Trp Thr Met Ser
370 375 380Val Phe Trp Arg Lys Val Arg Glu Leu Ser Arg Thr Leu Gln Gly Tyr385 390 395 400Gly Phe Trp Glu Asn Glu Asp Asp Met Leu Tyr Leu Asn Arg Thr Glu
405 410 415Val Arg Asp Val Leu Phe Asp Leu Ala Thr Ala Trp Gly Val Gly Ala
420 425 430Pro Gly Gly Pro Ile Gly Thr Ile Ile Trp Pro Glu Glu Ile Glu Arg
435 440 445Arg Lys Ala Ile Val Thr Ala Leu Lys Thr Ala Arg Pro Ala Pro Ala
450 455 460Leu Asn Thr Pro Pro Glu Ser Ile Thr Glu Pro Phe Thr Arg Met Leu465 470 475 480Trp Gly Ile Thr Thr Glu Gln Val Gln Ser Trp Leu Gly Asn Asp Glu
485 490 495Asp Ala Glu Glu Gly Thr Leu Lys Gly Met Ala Ala Ser Pro Gly Val
500 505 510Val Glu Gly Tyr Ala Arg Val Ile Leu Ser Ala Asp Asp Leu Ser Glu
515 520 525Ile Gln Gln Asp Glu Ile Leu Val Ala Pro Val Thr Ala Pro Ser Trp
530 535 540Gly Pro Ile Phe Gly Lys Ile Lys Ala Thr Val Thr Asp Ile Gly Gly545 550 555 560Met Met Ser His Ala Ala Ile Val Cys Arg Glu Tyr Gly Leu Pro Ala
565 570 575Val Thr Gly Thr Gly Ala Ala Ser Thr Thr Ile Lys Thr Gly Asp Tyr
580 585 590Leu Lys Val Asp Gly Thr Lys Gly Lys Val Val Ile Val Asp Pro Asp
595 600 605Ala Pro Arg Ile Glu Gly Pro Gly Ala His Ser His Ala His Ser Val
610 615 620Ala Ala His Gly Val Asp Thr His Ala625 630<210>83<211>1215<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1192)<223>RXA00683<400>83ggacaaagct atcgggttcc gggagaacct ctccttccgc gtccccactt ctgttcccgt 60gacttggaac gcttaacgct ttattaaata aggagacacc atg acc aac agt ttg 115
Met Thr Asn Ser Leu
1 5aac atc ccg ttt gtc cag cgc ttc gat gaa ggc ctg gat cct gtt cta 163Asn Ile Pro Phe Val Gln Arg Phe Asp Glu Gly Leu Asp Pro Val Leu
10 15 20gaa gta ctc ggt ggc aag ggc gct tca cta gtc acc atg aca gat gct 211Glu Val Leu Gly Gly Lys Gly Ala Ser Leu Val Thr Met Thr Asp Ala
25 30 35gga atg ccc gtt cca cct gga ttt gtg gtc act act gcc agc ttt gat 259Gly Met Pro Val Pro Pro Gly Phe Val Val Thr Thr Ala Ser Phe Asp
40 45 50gaa ttc atc cgt gaa gca ggg gtt gct gaa cac atc gat aaa ttc cta 307Glu Phe Ile Arg Glu Ala Gly Val Ala Glu His Ile Asp Lys Phe Leu
55 60 65aac gat ctc gat gca gaa gat gtt aag gaa gtg gat cga gtt tct gcg 355Asn Asp Leu Asp Ala Glu Asp Val Lys Glu Val Asp Arg Val Ser Ala70 75 80 85atc atc cgc gat gag ctg tgc agt ctt gac gtt cca gag aat gct cgt 403Ile Ile Arg Asp Glu Leu Cys Ser Leu Asp Val Pro Glu Asn Ala Arg
90 95 100ttc gca gtg cac cag gct tat cgc gat ctc atg gaa cga tgc ggt ggc 451Phe Ala Val His Gln Ala Tyr Arg Asp Leu Met Glu Arg Cys Gly Gly
105 110 115gac gtc ccg gtt gct gtc cgg tca tcg gcc act gcc gaa gat ctg ccc 499Asp Val Pro Val Ala Val Arg Ser Ser Ala Thr Ala Glu Asp Leu Pro
120 125 130gat gct tcc ttc gca ggg caa cag gac acc tat ctg tgg caa gtc ggt 547Asp Ala Ser Phe Ala Gly Gln Gln Asp Thr Tyr Leu Trp Gln Val Gly
135 140 145ttg agc gct gtc act gaa cac atc cgt aaa tgc tgg gct tcg ctg ttc 595Leu Ser Ala Val Thr Glu His Ile Arg Lys Cys Trp Ala Ser Leu Phe150 155 160 165act tcc cgt gcc att atc tac cgt ctg aaa aac aac atc ccc aat gag 643Thr Ser Arg Ala Ile Ile Tyr Arg Leu Lys Asn Asn Ile Pro Asn Glu
170 175 180ggc ctc tcc atg gcg gta gtt gtt caa aaa atg gtc aac tct cgt gtc 691Gly Leu Ser Met Ala Val Val Val Gln Lys Met Val Asn Ser Arg Val
185 190 195gca ggc gtg gca atc act atg aat cct tcc aac ggc gac cgc tcg aag 739Ala Gly Val Ala Ile Thr Met Asn Pro Ser Asn Gly Asp Arg Ser Lys
200 205 210atc acc atc gat tcc tca tgg ggt gtt ggt gaa atg gtg gtc tca ggt 787Ile Thr Ile Asp Ser Ser Trp Gly Val Gly Glu Met Val Val Ser Gly
215 220 225gaa gtg aca cca gac aat atc ttg ctg gac aag atc acg ctg cag gtt 835Glu Val Thr Pro Asp Asn Ile Leu Leu Asp Lys Ile Thr Leu Gln Val230 235 240 245gtc tcc gaa cac att gga agc aaa cac gct gaa ctc atc ccc gat gcc 883Val Ser Glu His Ile Gly Ser Lys His Ala Glu Leu Ile Pro Asp Ala
250 255 260acc agt gga agc ctc gtg gaa aag ccc gtt gat gaa gaa cgc gca aac 931Thr Ser Gly Ser Leu Val Glu Lys Pro Val Asp Glu Glu Arg Ala Asn
265 270 275cgc cgc agt ctg act gat gag gaa atg ctc gct gtg gca caa atg gct 979Arg Arg Ser Leu Thr Asp Glu Glu Met Leu Ala Val Ala Gln Met Ala
280 285 290aag cgt gca gaa aaa cac tac aag tgc cca caa gat atc gaa tgg gcg 1027Lys Arg Ala Glu Lys His Tyr Lys Cys Pro Gln Asp Ile Glu Trp Ala
295 300 305ctg gac gct gat ctg cca gat gga gaa aac ctt ctg tta ttg caa tcc 1075Leu Asp Ala Asp Leu Pro Asp Gly Glu Asn Leu Leu Leu Leu Gln Ser310 315 320 325cgc ccg gaa act atc cac tcc aac ggt gtg aag aag gaa acc cca act 1123Arg Pro Glu Thr Ile His Ser Asn Gly Val Lys Lys Glu Thr Pro Thr
330 335 340ccg cag gct gcc aaa acc ata ggc acc ttc gat ttc agc tca atc acc 1171Pro Gln Ala Ala Lys Thr Ile Gly Thr Phe Asp Phe Ser Ser Ile Thr
345 350 355gtc gca atg acc ggc acg aag taaaaccacc gcatcttttc gtc 1215Val Ala Met Thr Gly Thr Lys
360<210>84<211>364<212>PRT<213>谷氨酸棒杆菌<400>84Met Thr Asn Ser Leu Asn Ile Pro Phe Val Gln Arg Phe Asp Glu Gly1 5 10 15Leu Asp Pro Val Leu Glu Val Leu Gly Gly Lys Gly Ala Ser Leu Val
20 25 30Thr Met Thr Asp Ala Gly Met Pro Val Pro Pro Gly Phe Val Val Thr
35 40 45Thr Ala Ser Phe Asp Glu Phe Ile Arg Glu Ala Gly Val Ala Glu His
50 55 60Ile Asp Lys Phe Leu Asn Asp Leu Asp Ala Glu Asp Val Lys Glu Val65 70 75 80Asp Arg Val Ser Ala Ile Ile Arg Asp Glu Leu Cys Ser Leu Asp Val
85 90 95Pro Glu Asn Ala Arg Phe Ala Val His Gln Ala Tyr Arg Asp Leu Met
100 105 110Glu Arg Cys Gly Gly Asp Val Pro Val Ala Val Arg Ser Ser Ala Thr
115 120 125Ala Glu Asp Leu Pro Asp Ala Ser Phe Ala Gly Gln Gln Asp Thr Tyr
130 135 140Leu Trp Gln Val Gly Leu Ser Ala Val Thr Glu His Ile Arg Lys Cys145 150 155 160Trp Ala Ser Leu Phe Thr Ser Arg Ala Ile Ile Tyr Arg Leu Lys Asn
165 170 175Asn Ile Pro Asn Glu Gly Leu Ser Met Ala Val Val Val Gln Lys Met
180 185 190Val Asn Ser Arg Val Ala Gly Val Ala Ile Thr Met Asn Pro Ser Asn
195 200 205Gly Asp Arg Ser Lys Ile Thr Ile Asp Ser Ser Trp Gly Val Gly Glu
210 215 220Met Val Val Ser Gly Glu Val Thr Pro Asp Asn Ile Leu Leu Asp Lys225 230 235 240Ile Thr Leu Gln Val Val Ser Glu His Ile Gly Ser Lys His Ala Glu
245 250 255Leu Ile Pro Asp Ala Thr Ser Gly Ser Leu Val Glu Lys Pro Val Asp
260 265 270Glu Glu Arg Ala Asn Arg Arg Ser Leu Thr Asp Glu Glu Met Leu Ala
275 280 285Val Ala Gln Met Ala Lys Arg Ala Glu Lys His Tyr Lys Cys Pro Gln
290 295 300Asp Ile Glu Trp Ala Leu Asp Ala Asp Leu Pro Asp Gly Glu Asn Leu305 310 315 320Leu Leu Leu Gln Ser Arg Pro Glu Thr Ile His Ser Asn Gly Val Lys
325 330 335Lys Glu Thr Pro Thr Pro Gln Ala Ala Lys Thr Ile Gly Thr Phe Asp
340 345 350Phe Ser Ser Ile Thr Val Ala Met Thr Gly Thr Lys
355 360<210>85<211>1860<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1837)<223>RXN00635<400>85ctggcaggcg ggcgaagcgt ggcaacaact ggaatttaag agcacaattg aagtcgcacc 60aagttaggca acacaatagc cataacgttg aggagttcag atg gca cac agc tac 115
Met Ala His Ser Tyr
1 5gca gaa caa tta att gac act ttg gaa gct caa ggt gtg aag cga att 163Ala Glu Gln Leu Ile Asp Thr Leu Glu Ala Gln Gly Val Lys Arg Ile
10 15 20tat ggt ttg gtg ggt gac agc ctt aat ccg atc gtg gat gct gtc cgc 211Tyr Gly Leu Val Gly Asp Ser Leu Asn Pro Ile Val Asp Ala Val Arg
25 30 35caa tca gat att gag tgg gtg cac gtt cga aat gag gaa gcg gcg gcg 259Gln Ser Asp Ile Glu Trp Val His Val Arg Asn Glu Glu Ala Ala Ala
40 45 50ttt gca gcc ggt gcg gaa tcg ttg atc act ggg gag ctg gca gta tgt 307Phe Ala Ala Gly Ala Glu Ser Leu Ile Thr Gly Glu Leu Ala Val Cys
55 60 65gct gct tct tgt ggt cct gga aac aca cac ctg att cag ggt ctt tat 355Ala Ala Ser Cys Gly Pro Gly Asn Thr His Leu Ile Gln Gly Leu Tyr70 75 80 85gat tcg cat cga aat ggt gcg aag gtg ttg gcc atc gct agc cat att 403Asp Ser His Arg Asn Gly Ala Lys Val Leu Ala Ile Ala Ser His Ile
90 95 100ccg agt gcc cag att ggt tcg acg ttc ttc cag gaa acg cat ccg gag 451Pro Ser Ala Gln Ile Gly Ser Thr Phe Phe Gln Glu Thr His Pro Glu
105 110 115att ttg ttt aag gaa tgc tct ggt tac tgc gag atg gtg aat ggt ggt 499Ile Leu Phe Lys Glu Cys Ser Gly Tyr Cys Glu Met Val Asn Gly Gly
120 125 130gag cag ggt gaa cgc att ttg cat cac gcg att cag tcc acc atg gcg 547Glu Gln Gly Glu Arg Ile Leu His His Ala Ile Gln Ser Thr Met Ala
135 140 145ggt aaa ggt gtg tcg gtg gta gtg att cct ggt gat atc gct aag gaa 595Gly Lys Gly Val Ser Val Val Val Ile Pro Gly Asp Ile Ala Lys Glu150 155 160 165gac gca ggt gac ggt act tat tcc aat tcc act att tct tct ggc act 643Asp Ala Gly Asp Gly Thr Tyr Ser Asn Ser Thr Ile Ser Ser Gly Thr
170 175 180cct gtg gtg ttc ccg gat cct act gag gct gca gcg ctg gtg gag gcg 691Pro Val Val Phe Pro Asp Pro Thr Glu Ala Ala Ala Leu Val Glu Ala
185 190 195att aac aac gct aag tct gtc act ttg ttc tgc ggt gcg ggc gtg aag 739Ile Asn Asn Ala Lys Ser Val Thr Leu Phe Cys Gly Ala Gly Val Lys
200 205 210aat gct cgc gcg cag gtg ttg gag ttg gcg gag aag att aaa tca ccg 787Asn Ala Arg Ala Gln Val Leu Glu Leu Ala Glu Lys Ile Lys Ser Pro
215 220 225atc ggg cat gcg ctg ggt ggt aag cag tac atc cag cat gag aat ccg 835Ile Gly His Ala Leu Gly Gly Lys Gln Tyr Ile Gln His Glu Asn Pro230 235 240 245ttt gag gtc ggc atg tct ggc ctg ctt ggt tac ggc gcc tgc gtg gat 883Phe Glu Val Gly Met Ser Gly Leu Leu Gly Tyr Gly Ala Cys Val Asp
250 255 260gcg tcc aat gag gcg gat ctg ctg att cta ttg ggt acg gat ttc cct 931Ala Ser Asn Glu Ala Asp Leu Leu Ile Leu Leu Gly Thr Asp Phe Pro
265 270 275tat tct gat ttc ctt cct aaa gac aac gtt gcc cag gtg gat atc aac 979Tyr Ser Asp Phe Leu Pro Lys Asp Asn Val Ala Gln Val Asp Ile Asn
280 285 290ggt gcg cac att ggt cga cgt acc acg gtg aag tat ccg gtg acc ggt 1027Gly Ala His Ile Gly Arg Arg Thr Thr Val Lys Tyr Pro Val Thr Gly
295 300 305gat gtt gct gca aca atc gaa aat att ttg cct cat gtg aag gaa aaa 1075Asp Val Ala Ala Thr Ile Glu Asn Ile Leu Pro His Val Lys Glu Lys310 315 320 325aca gat cgt tcc ttc ctt gat cgg atg ctc aag gca cac gag cgt aag 1123Thr Asp Arg Ser Phe Leu Asp Arg Met Leu Lys Ala His Glu Arg Lys
330 335 340ttg agc tcg gtg gta gag acg tac aca cat aac gtc gag aag cat gtg 1171Leu Ser Ser Val Val Glu Thr Tyr Thr His Asn Val Glu Lys His Val
345 350 355cct att cac cct gaa tac gtt gcc tct att ttg aac gag ctg gcg gat 1219Pro Ile His Pro Glu Tyr Val Ala Ser Ile Leu Asn Glu Leu Ala Asp
360 365 370aag gat gcg gtg ttt act gtg gat acc ggc atg tgc aat gtg tgg cat 1267Lys Asp Ala Val Phe Thr Val Asp Thr Gly Met Cys Asn Val Trp His
375 380 385gcg agg tac atc gag aat ccg gag gga acg cgc gac ttt gtg ggt tca 1315Ala Arg Tyr Ile Glu Asn Pro Glu Gly Thr Arg Asp Phe Val Gly Ser390 395 400 405ttc cgc cac ggc acg atg gct aat gcg ttg cct cat gcg att ggt gcg 1363Phe Arg His Gly Thr Met Ala Asn Ala Leu Pro His Ala Ile Gly Ala
410 415 420caa agt gtt gat cga aac cgc cag gtg atc gcg atg tgt ggc gat ggt 1411Gln Ser Val Asp Arg Asn Arg Gln Val Ile Ala Met Cys Gly Asp Gly
425 430 435ggt ttg ggc atg ctg ctg ggt gag ctt ctg acc gtt aag ctg cac caa 1459Gly Leu Gly Met Leu Leu Gly Glu Leu Leu Thr Val Lys Leu His Gln
440 445 450ctt ccg ctg aag gct gtg gtg ttt aac aac agt tct ttg ggc atg gtg 1507Leu Pro Leu Lys Ala Val Val Phe Asn Asn Ser Ser Leu Gly Met Val
455 460 465aag ttg gag atg ctc gtg gag gga cag cca gaa ttt ggt act gac cat 1555Lys Leu Glu Met Leu Val Glu Gly Gln Pro Glu Phe Gly Thr Asp His470 475 480 485gag gaa gtg aat ttc gca gag att gcg gcg gct gcg ggt atc aaa tcg 1603Glu Glu Val Asn Phe Ala Glu Ile Ala Ala Ala Ala Gly Ile Lys Ser
490 495 500gta cgc atc acc gat ccg aag aaa gtt cgc gag cag cta gct gag gca 1651Val Arg Ile Thr Asp Pro Lys Lys Val Arg Glu Gln Leu Ala Glu Ala
505 510 515ttg gca tat cct gga cct gta ctg atc gat atc gtc acg gat cct aat 1699Leu Ala Tyr Pro Gly Pro Val Leu Ile Asp Ile Val Thr Asp Pro Asn
520 525 530gcg ctg tcg atc cca cca acc atc acg tgg gaa cag gtc atg gga ttc 1747Ala Leu Ser Ile Pro Pro Thr Ile Thr Trp Glu Gln Val Met Gly Phe
535 540 545agc aag gcg gcc acc cga acc gtc ttt ggt gga gga gta gga gcg atg 1795Ser Lys Ala Ala Thr Arg Thr Val Phe Gly Gly Gly Val Gly Ala Met550 555 560 565atc gat ctg gcc cgt tcg aac ata agg aat att cct act cca 1837Ile Asp Leu Ala Arg Ser Asn Ile Arg Asn Ile Pro Thr Pro
570 575tgatgattga tacacctgct gtt 1860<210>86<211>579<212>PRT<213>谷氨酸棒杆菌<400>86Met Ala His Ser Tyr Ala Glu Gln Leu Ile Asp Thr Leu Glu Ala Gln1 5 10 15Gly Val Lys Arg Ile Tyr Gly Leu Val Gly Asp Ser Leu Asn Pro Ile
20 25 30Val Asp Ala Val Arg Gln Ser Asp Ile Glu Trp Val His Val Arg Asn
35 40 45Glu Glu Ala Ala Ala Phe Ala Ala Gly Ala Glu Ser Leu Ile Thr Gly
50 55 60Glu Leu Ala Val Cys Ala Ala Ser Cys Gly Pro Gly Asn Thr His Leu65 70 75 80Ile Gln Gly Leu Tyr Asp Ser His Arg Asn Gly Ala Lys Val Leu Ala
85 90 95Ile Ala Ser His Ile Pro Ser Ala Gln Ile Gly Ser Thr Phe Phe Gln
100 105 110Glu Thr His Pro Glu Ile Leu Phe Lys Glu Cys Ser Gly Tyr Cys Glu
115 120 125Met Val Asn Gly Gly Glu Gln Gly Glu Arg Ile Leu His His Ala Ile
130 135 140Gln Ser Thr Met Ala Gly Lys Gly Val Ser Val Val Val Ile Pro Gly145 150 155 160Asp Ile Ala Lys Glu Asp Ala Gly Asp Gly Thr Tyr Ser Asn Ser Thr
165 170 175Ile Ser Ser Gly Thr Pro Val Val Phe Pro Asp Pro Thr Glu Ala Ala
180 185 190Ala Leu Val Glu Ala Ile Asn Asn Ala Lys Ser Val Thr Leu Phe Cys
195 200 205Gly Ala Gly Val Lys Asn Ala Arg Ala Gln Val Leu Glu Leu Ala Glu
210 215 220Lys Ile Lys Ser Pro Ile Gly His Ala Leu Gly Gly Lys Gln Tyr Ile225 230 235 240Gln His Glu Asn Pro Phe Glu Val Gly Met Ser Gly Leu Leu Gly Tyr
245 250 255Gly Ala Cys Val Asp Ala Ser Asn Glu Ala Asp Leu Leu Ile Leu Leu
260 265 270Gly Thr Asp Phe Pro Tyr Ser Asp Phe Leu Pro Lys Asp Asn Val Ala
275 280 285Gln Val Asp Ile Asn Gly Ala His Ile Gly Arg Arg Thr Thr Val Lys
290 295 300Tyr Pro Val Thr Gly Asp Val Ala Ala Thr Ile Glu Asn Ile Leu Pro305 310 315 320His Val Lys Glu Lys Thr Asp Arg Ser Phe Leu Asp Arg Met Leu Lys
325 330 335Ala His Glu Arg Lys Leu Ser Ser Val Val Glu Thr Tyr Thr His Asn
340 345 350Val Glu Lys His Val Pro Ile His Pro Glu Tyr Val Ala Ser Ile Leu
355 360 365Asn Glu Leu Ala Asp Lys Asp Ala Val Phe Thr Val Asp Thr Gly Met
370 375 380Cys Asn Val Trp His Ala Arg Tyr Ile Glu Asn Pro Glu Gly Thr Arg385 390 395 400Asp Phe Val Gly Ser Phe Arg His Gly Thr Met Ala Asn Ala Leu Pro
405 410 415His Ala Ile Gly Ala Gln Ser Val Asp Arg Asn Arg Gln Val Ile Ala
420 425 430Met Cys Gly Asp Gly Gly Leu Gly Met Leu Leu Gly Glu Leu Leu Thr
435 440 445Val Lys Leu His Gln Leu Pro Leu Lys Ala Val Val Phe Asn Asn Ser
450 455 460Ser Leu Gly Met Val Lys Leu Glu Met Leu Val Glu Gly Gln Pro Glu465 470 475 480Phe Gly Thr Asp His Glu Glu Val Asn Phe Ala Glu Ile Ala Ala Ala
485 490 495Ala Gly Ile Lys Ser Val Arg Ile Thr Asp Pro Lys Lys Val Arg Glu
500 505 510Gln Leu Ala Glu Ala Leu Ala Tyr Pro Gly Pro Val Leu Ile Asp Ile
515 520 525Val Thr Asp Pro Asn Ala Leu Ser Ile Pro Pro Thr Ile Thr Trp Glu
530 535 540Gln Val Met Gly Phe Ser Lys Ala Ala Thr Arg Thr Val Phe Gly Gly545 550 555 560Gly Val Gly Ala Met Ile Asp Leu Ala Arg Ser Asn Ile Arg Asn Ile
565 570 575Pro Thr Pro<210>87<211>552<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(88)..(552)<223>FRXA02807<400>87aaagcgtggc aacaactgga atttaagagc acaattgaag tcgcaccaag ttaggcaaca 60caatagccat aacgttgagg agttcag atg gca cac agc tac gca gaa caa tta 114
Met Ala His Ser Tyr Ala Glu Gln Leu
1 5att gac act ttg gaa gct caa ggt gtg aag cga att tat ggt ttg gtg 162Ile Asp Thr Leu Glu Ala Gln Gly Val Lys Arg Ile Tyr Gly Leu Val10 15 20 25ggt gac agc ctt aat ccg atc gtg gat gct gtc cgc caa tca gat att 210Gly Asp Ser Leu Asn Pro Ile Val Asp Ala Val Arg Gln Ser Asp Ile
30 35 40gag tgg gtg cac gtt cga aat gag gaa gcg gcg gcg ttt gca gcc ggt 258Glu Trp Val His Val Arg Asn Glu Glu Ala Ala Ala Phe Ala Ala Gly
45 50 55gcg gaa tcg ttg atc act ggg gag ctg gca gta tgt gct gct tct tgt 306Ala Glu Ser Leu Ile Thr Gly Glu Leu Ala Val Cys Ala Ala Ser Cys
60 65 70ggt cct gga aac aca cac ctg att cag ggt ctt tat gat tcg cat cga 354Gly Pro Gly Asn Thr His Leu Ile Gln Gly Leu Tyr Asp Ser His Arg
75 80 85aat ggt gcg aag gtg ttg gcc atc gct agc cat att ccg agt gcc cag 402Asn Gly Ala Lys Val Leu Ala Ile Ala Ser His Ile Pro Ser Ala Gln90 95 100 105att ggt tcg acg ttc ttc cag gaa acg cat ccg gag att ttg ttt aag 450Ile Gly Ser Thr Phe Phe Gln Glu Thr His Pro Glu Ile Leu Phe Lys
110 115 120gaa tgc tct ggt tac tgc gag atg gtg aat ggt ggt gag cag ggt gaa 498Glu Cys Ser Gly Tyr Cys Glu Met Val Asn Gly Gly Glu Gln Gly Glu
125 130 135cgc att ttg cat cac gcg att cag tcc acc atg gcg ggt aaa ggt gtg 546Arg Ile Leu His His Ala Ile Gln Ser Thr Met Ala Gly Lys Gly Val
140 145 150tcg gtg 552Ser Val
155<210>88<211>155<212>PRT<213>谷氨酸棒杆菌<400>88Met Ala His Ser Tyr Ala Glu Gln Leu Ile Asp Thr Leu Glu Ala Gln1 5 10 15Gly Val Lys Arg Ile Tyr Gly Leu Val Gly Asp Ser Leu Asn Pro Ile
20 25 30Val Asp Ala Val Arg Gln Ser Asp Ile Glu Trp Val His Val Arg Asn
35 40 45Glu Glu Ala Ala Ala Phe Ala Ala Gly Ala Glu Ser Leu Ile Thr Gly
50 55 60Glu Leu Ala Val Cys Ala Ala Ser Cys Gly Pro Gly Asn Thr His Leu65 70 75 80Ile Gln Gly Leu Tyr Asp Ser His Arg Asn Gly Ala Lys Val Leu Ala
85 90 95Ile Ala Ser His Ile Pro Ser Ala Gln Ile Gly Ser Thr Phe Phe Gln
100 105 110Glu Thr His Pro Glu Ile Leu Phe Lys Glu Cys Ser Gly Tyr Cys Glu
115 120 125Met Val Asn Gly Gly Glu Gln Gly Glu Arg Ile Leu His His Ala Ile
130 135 140Gln Ser Thr Met Ala Gly Lys Gly Val Ser Val145 150 155<210>89<211>944<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(921)<223>FRXA00635<400>89ggt acg gat ttc cct tat tct gat ttc ctt cct aaa gac aac gtt gcc 48Gly Thr Asp Phe Pro Tyr Ser Asp Phe Leu Pro Lys Asp Asn Val Ala1 5 10 15cag gtg gat atc aac ggt gcg cac att ggt cga cgt acc acg gtg aag 96Gln Val Asp Ile Asn Gly Ala His Ile Gly Arg Arg Thr Thr Val Lys
20 25 30tat ccg gtg acc ggt gat gtt gct gca aca atc gaa aat att ttg cct 144Tyr Pro Val Thr Gly Asp Val Ala Ala Thr Ile Glu Asn Ile Leu Pro
35 40 45cat gtg aag gaa aaa aca gat cgt tcc ttc ctt gat cgg atg ctc aag 192His Val Lys Glu Lys Thr Asp Arg Ser Phe Leu Asp Arg Met Leu Lys
50 55 60gca cac gag cgt aag ttg agc tcg gtg gta gag acg tac aca cat aac 240Ala His Glu Arg Lys Leu Ser Ser Val Val Glu Thr Tyr Thr His Asn65 70 75 80gtc gag aag cat gtg cct att cac cct gaa tac gtt gcc tct att ttg 288Val Glu Lys His Val Pro Ile His Pro Glu Tyr Val Ala Ser Ile Leu
85 90 95aac gag ctg gcg gat aag gat gcg gtg ttt act gtg gat acc ggc atg 336Asn Glu Leu Ala Asp Lys Asp Ala Val Phe Thr Val Asp Thr Gly Met
100 105 110tgc aat gtg tgg cat gcg agg tac atc gag aat ccg gag gga acg cgc 384Cys Asn Val Trp His Ala Arg Tyr Ile Glu Asn Pro Glu Gly Thr Arg
115 120 125gac ttt gtg ggt tca ttc cgc cac ggc acg atg gct aat gcg ttg cct 432Asp Phe Val Gly Ser Phe Arg His Gly Thr Met Ala Asn Ala Leu Pro
130 135 140cat gcg att ggt gcg caa agt gtt gat cga aac cgc cag gtg atc gcg 480His Ala Ile Gly Ala Gln Ser Val Asp Arg Asn Arg Gln Val Ile Ala145 150 155 160atg tgt ggc gat ggt ggt ttg ggc atg ctg ctg ggt gag ctt ctg acc 528Met Cys Gly Asp Gly Gly Leu Gly Met Leu Leu Gly Glu Leu Leu Thr
165 170 175gtt aag ctg cac caa ctt ccg ctg aag gct gtg gtg ttt aac aac agt 576Val Lys Leu His Gln Leu Pro Leu Lys Ala Val Val Phe Asn Asn Ser
180 185 190tct ttg ggc atg gtg aag ttg gag atg ctc gtg gag gga cag cca gaa 624Ser Leu Gly Met Val Lys Leu Glu Met Leu Val Glu Gly Gln Pro Glu
195 200 205ttt ggt act gac cat gag gaa gtg aat ttc gca gag att gcg gcg gct 672Phe Gly Thr Asp His Glu Glu Val Asn Phe Ala Glu Ile Ala Ala Ala
210 215 220gcg ggt atc aaa tcg gta cgc atc acc gat ccg aag aaa gtt cgc gag 720Ala Gly Ile Lys Ser Val Arg Ile Thr Asp Pro Lys Lys Val Arg Glu225 230 235 240cag cta gct gag gca ttg gca tat cct gga cct gta ctg atc gat atc 768Gln Leu Ala Glu Ala Leu Ala Tyr Pro Gly Pro Val Leu Ile Asp Ile
245 250 255gtc acg gat cct aat gcg ctg tcg atc cca cca acc atc acg tgg gaa 816Val Thr Asp Pro Asn Ala Leu Ser Ile Pro Pro Thr Ile Thr Trp Glu
260 265 270cag gtc atg gga ttc agc aag gcg gcc acc cga acc gtc ttt ggt gga 864Gln Val Met Gly Phe Ser Lys Ala Ala Thr Arg Thr Val Phe Gly Gly
275 280 285gga gta gga gcg atg atc gat ctg gcc cgt tcg aac ata agg aat att 912Gly Val Gly Ala Met Ile Asp Leu Ala Arg Ser Asn Ile Arg Asn Ile
290 295 300cct act cca tgatgattga tacacctgct gtt 944Pro Thr Pro305<210>90<211>307<212>PRT<213>谷氨酸棒杆菌<400>90Gly Thr Asp Phe Pro Tyr Ser Asp Phe Leu Pro Lys Asp Asn Val Ala1 5 10 15Gln Val Asp Ile Asn Gly Ala His Ile Gly Arg Arg Thr Thr Val Lys
20 25 30Tyr Pro Val Thr Gly Asp Val Ala Ala Thr Ile Glu Asn Ile Leu Pro
35 40 45His Val Lys Glu Lys Thr Asp Arg Ser Phe Leu Asp Arg Met Leu Lys
50 55 60Ala His Glu Arg Lys Leu Ser Ser Val Val Glu Thr Tyr Thr His Asn65 70 75 80Val Glu Lys His Val Pro Ile His Pro Glu Tyr Val Ala Ser Ile Leu
85 90 95Asn Glu Leu Ala Asp Lys Asp Ala Val Phe Thr Val Asp Thr Gly Met
100 105 110Cys Asn Val Trp His Ala Arg Tyr Ile Glu Asn Pro Glu Gly Thr Arg
115 120 125Asp Phe Val Gly Ser Phe Arg His Gly Thr Met Ala Asn Ala Leu Pro
130 135 140His Ala Ile Gly Ala Gln Ser Val Asp Arg Asn Arg Gln Val Ile Ala145 150 155 160Met Cys Gly Asp Gly Gly Leu Gly Met Leu Leu Gly Glu Leu Leu Thr
165 170 175Val Lys Leu His Gln Leu Pro Leu Lys Ala Val Val Phe Asn Asn Ser
180 185 190Ser Leu Gly Met Val Lys Leu Glu Met Leu Val Glu Gly Gln Pro Glu
195 200 205Phe Gly Thr Asp His Glu Glu Val Asn Phe Ala Glu Ile Ala Ala Ala
210 215 220Ala Gly Ile Lys Ser Val Arg Ile Thr Asp Pro Lys Lys Val Arg Glu225 230 235 240Gln Leu Ala Glu Ala Leu Ala Tyr Pro Gly Pro Val Leu Ile Asp Ile
245 250 255Val Thr Asp Pro Asn Ala Leu Ser Ile Pro Pro Thr Ile Thr Trp Glu
260 265 270Gln Val Met Gly Phe Ser Lys Ala Ala Thr Arg Thr Val Phe Gly Gly
275 280 285Gly Val Gly Ala Met Ile Asp Leu Ala Arg Ser Asn Ile Arg Asn Ile
290 295 300Pro Thr Pro305<210>91<211>954<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(931)<223>RXN03044<400>91tacgccaccc acggcaaggc catgattccg ctgtacatct tctactcgat gttcgggatt 60ccagcgcacc ggtgactcca tctgggcagc agccgatcag atg gca cgt ggc ttc 115
Met Ala Arg Gly Phe
1 5ctc ttg ggc gct acc gca ggt cgc acc acc ctg acc ggt gaa ggc ctc 163Leu Leu Gly Ala Thr Ala Gly Arg Thr Thr Leu Thr Gly Glu Gly Leu
10 15 20cag cac atg gat gga cac tcc cct gtc ttg gct tcc acc aac gag ggt 211Gln His Met Asp Gly His Ser Pro Val Leu Ala Ser Thr Asn Glu Gly
25 30 35gtc gag acc tac gac cca tcc ttt gcg tac gag atc gca cac ctg gtt 259Val Glu Thr Tyr Asp Pro Ser Phe Ala Tyr Glu Ile Ala His Leu Val
40 45 50cac cgt ggc atc gac cgc atg tac ggc cca ggc aag ggt gaa gat gtt 307His Arg Gly Ile Asp Arg Met Tyr Gly Pro Gly Lys Gly Glu Asp Val
55 60 65atc tac tac atc acc atc tac aac gag cca acc cca cag cca gct gag 355Ile Tyr Tyr Ile Thr Ile Tyr Asn Glu Pro Thr Pro Gln Pro Ala Glu70 75 80 85cca gaa gga ctg gac gta gaa ggc ctg cac aag ggc atc tac ctc tac 403Pro Glu Gly Leu Asp Val Glu Gly Leu His Lys Gly Ile Tyr Leu Tyr
90 95 100tcc cgc ggt gaa ggc acc ggc cat gag gca aac atc ttg gct tcc ggt 451Ser Arg Gly Glu Gly Thr Gly His Glu Ala Asn Ile Leu Ala Ser Gly
105 110 115gtt ggt atg cag tgg gct ctc aag gct gca tcc atc ctt gag gct gac 499Val Gly Met Gln Try Ala Leu Lys Ala Ala Ser Ile Leu Glu Ala Asp
120 125 130tac gga gtt cgt gcc aac att tac tcc gct act tct tgg gtt aac ttg 547Tyr Gly Val Arg Ala Asn Ile Tyr Ser Ala Thr Ser Trp Val Asn Leu
135 140 145gct cgc gat ggc gct gct cgt aac aag gca cag ctg cgc aac cca ggt 595Ala Arg Asp Gly Ala Ala Arg Asn Lys Ala Gln Leu Arg Asn Pro Gly150 155 160 165gca gat gct ggc gag gca ttc gta acc acc cag ctg aag cag acc tcc 643Ala Asp Ala Gly Glu Ala Phe Val Thr Thr Gln Leu Lys Gln Thr Ser
170 175 180ggc cca tac gtt gca gtg tct gac ttc tcc act gat ctg cca aac cag 691Gly Pro Tyr Val Ala Val Ser Asp Phe Ser Thr Asp Leu Pro Asn Gln
185 190 195atc cgt gaa tgg gtc cca ggc gac tac acc gtt ctc ggt gca gat ggc 739Ile Arg Glu Trp Val Pro Gly Asp Tyr Thr Val Leu Gly Ala Asp Gly
200 205 210ttc ggt ttc tct gat acc cgc cca gct gct cgt cgc ttc ttc aac atc 787Phe Gly Phe Ser Asp Thr Arg Pro Ala Ala Arg Arg Phe Phe Asn Ile
215 220 225gac gct gag tcc att gtt gtt gca gtg ctg aac tcc ctg gca cgc gaa 835Asp Ala Glu Ser Ile Val Val Ala Val Leu Asn Ser Leu Ala Arg Glu230 235 240 245ggc aag atc gac gtc tcc gtt gct gct cag gct gct gag aag ttc aag 883Gly Lys Ile Asp Val Ser Val Ala Ala Gln Ala Ala Glu Lys Phe Lys
250 255 260ttg gat gat cct acg agt gtt tcc gta gat cca aac gct cct gag gaa 931Leu Asp Asp Pro Thr Ser Val Ser Val Asp Pro Asn Ala Pro Glu Glu
265 270 275taaatcacct caagggacag ata 954<210>92<211>277<212>PRT<213>谷氨酸棒杆菌<400>92Met Ala Arg Gly Phe Leu Leu Gly Ala Thr Ala Gly Arg Thr Thr Leu1 5 10 15Thr Gly Glu Gly Leu Gln His Met Asp Gly His Ser Pro Val Leu Ala
20 25 30Ser Thr Asn Glu Gly Val Glu Thr Tyr Asp Pro Ser Phe Ala Tyr Glu
35 40 45Ile Ala His Leu Val His Arg Gly Ile Asp Arg Met Tyr Gly Pro Gly
50 55 60Lys Gly Glu Asp Val Ile Tyr Tyr Ile Thr Ile Tyr Asn Glu Pro Thr65 70 75 80Pro Gln Pro Ala Glu Pro Glu Gly Leu Asp Val Glu Gly Leu His Lys
85 90 95Gly Ile Tyr Leu Tyr Ser Arg Gly Glu Gly Thr Gly His Glu Ala Asn
100 105 110Ile Leu Ala Ser Gly Val Gly Met Gln Trp Ala Leu Lys Ala Ala Ser
115 120 125Ile Leu Glu Ala Asp Tyr Gly Val Arg Ala Asn Ile Tyr Ser Ala Thr
130 135 140Ser Trp Val Asn Leu Ala Arg Asp Gly Ala Ala Arg Asn Lys Ala Gln145 150 155 160Leu Arg Asn Pro Gly Ala Asp Ala Gly Glu Ala Phe Val Thr Thr Gln
165 170 175Leu Lys Gln Thr Ser Gly Pro Tyr Val Ala Val Ser Asp Phe Ser Thr
180 185 190Asp Leu Pro Asn Gln Ile Arg Glu Trp Val Pro Gly Asp Tyr Thr Val
195 200 205Leu Gly Ala Asp Gly Phe Gly Phe Ser Asp Thr Arg Pro Ala Ala Arg
210 215 220Arg Phe Phe Asn Ile Asp Ala Glu Ser Ile Val Val Ala Val Leu Asn225 230 235 240Ser Leu Ala Arg Glu Gly Lys Ile Asp Val Ser Val Ala Ala Gln Ala
245 250 255Ala Glu Lys Phe Lys Leu Asp Asp Pro Thr Ser Val Ser Val Asp Pro
260 265 270Asn Ala Pro Glu Glu
275<210>93<211>302<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(279)<223>FRXA02852<400>93gtt gca gtg tct gac ttc tcc act gat ctg cca aac cag atc cgt gaa 48Val Ala Val Ser Asp Phe Ser Thr Asp Leu Pro Asn Gln Ile Arg Glu1 5 10 15tgg gtc cca ggc gac tac acc gtt ctc ggt gca gat ggc ttc ggt ttc 96Trp Val Pro Gly Asp Tyr Thr Val Leu Gly Ala Asp Gly Phe Gly Phe
20 25 30tct gat acc cgc cca gct gct cgt cgc ttc ttc aac atc gac gct gag 144Ser Asp Thr Arg Pro Ala Ala Arg Arg Phe Phe Asn Ile Asp Ala Glu
35 40 45tcc att gtt gtt gca gtg ctg aac tcc ctg gca cgc gaa ggc aag atc 192Ser Ile Val Val Ala Val Leu Asn Ser Leu Ala Arg Glu Gly Lys Ile
50 55 60gac gtc tcc gtt gct gct cag gct gct gag aag ttc aag ttg gat gat 240Asp Val Ser Val Ala Ala Gln Ala Ala Glu Lys Phe Lys Leu Asp Asp65 70 75 80cct acg agt gtt tcc gta gat cca aac gct cct gag gaa taaatcacct 289Pro Thr Ser Val Ser Val Asp Pro Asn Ala Pro Glu Glu
85 90caagggacag ata 302<210>94<211>93<212>PRT<213>谷氨酸棒杆菌<400>94Val Ala Val Ser Asp Phe Ser Thr Asp Leu Pro Asn Gln Ile Arg Glu1 5 10 15Trp Val Pro Gly Asp Tyr Thr Val Leu Gly Ala Asp Gly Phe Gly Phe
20 25 30Ser Asp Thr Arg Pro Ala Ala Arg Arg Phe Phe Asn Ile Asp Ala Glu
35 40 45Ser Ile Val Val Ala Val Leu Asn Ser Leu Ala Arg Glu Gly Lys Ile
50 55 60Asp Val Ser Val Ala Ala Gln Ala Ala Glu Lys Phe Lys Leu Asp Asp65 70 75 80Pro Thr Ser Val Ser Val Asp Pro Asn Ala Pro Glu Glu
85 90<210>95<211>954<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(931)<223>FRXA00268<400>95tacgccaccc acggcaaggc catgattccg ctgtacatct tctactcgat gttcgggatt 60ccagcgcacc ggtgactcca tttgggcagc agccgatcag atg gca cgt ggc ttc 115
Met Ala Arg Gly Phe
1 5ttt ttg ggc gct acc gca ggt cgc acc acc ttg acc ggt gaa ggc ctc 163Phe Leu Gly Ala Thr Ala Gly Arg Thr Thr Leu Thr Gly Glu Gly Leu
10 15 20cag cac atg gat gga cac tcc cct gtt ttg gct tcc acc aac gag ggt 211Gln His Met Asp Gly His Ser Pro Val Leu Ala Ser Thr Asn Glu Gly
25 30 35gtc gag acc tac gac cca tcc ttt gcg tac gag atc gca cac ctg gtt 259Val Glu Thr Tyr Asp Pro Ser Phe Ala Tyr Glu Ile Ala His Leu Val
40 45 50cac cgt ggc atc gac cgc atg tac ggc cca ggc aag ggt gaa gat gtt 307His Arg Gly Ile Asp Arg Met Tyr Gly Pro Gly Lys Gly Glu Asp Val
55 60 65atc tac tac atc acc atc tac aac gag cca acc cca cag cca gct gag 355Ile Tyr Tyr Ile Thr Ile Tyr Asn Glu Pro Thr Pro Gln Pro Ala Glu70 75 80 85cca gaa gga ctg gac gta gaa ggc ctg cac aag ggc atc tac ctc tac 403Pro Glu Gly Leu Asp Val Glu Gly Leu His Lys Gly Ile Tyr Leu Tyr
90 95 100tcc cgc ggt gaa ggc acc ggc cat gag gca aac atc ttg gct tcc ggt 451Ser Arg Gly Glu Gly Thr Gly His Glu Ala Asn Ile Leu Ala Ser Gly
105 110 115gtt ggt atg cag tgg gct ctc aag gct gca tcc atc ctt gag gct gac 499Val Gly Met Gln Trp Ala Leu Lys Ala Ala Ser Ile Leu Glu Ala Asp
120 125 130tac gga gtt cgt gcc aac att tac tcc gct act tct tgg gtt aac ttg 547Tyr Gly Val Arg Ala Asn Ile Tyr Ser Ala Thr Ser Trp Val Asn Leu
135 140 145gct cgc gat ggc gct gct cgt aac aag gca cag ctg cgc aac cca ggt 595Ala Arg Asp Gly Ala Ala Arg Asn Lys Ala Gln Leu Arg Asn Pro Gly150 155 160 165gca gat gct ggc gag gca ttc gta acc acc cag ctg aag cag acc tcc 643Ala Asp Ala Gly Glu Ala Phe Val Thr Thr Gln Leu Lys Gln Thr Ser
170 175 180ggc cca tac gtt gca gtg tct gac ttc tcc act gat ctg cca aac cag 691Gly Pro Tyr Val Ala Val Ser Asp Phe Ser Thr Asp Leu Pro Asn Gln
185 190 195atc cgt gaa tgg gtc cca ggc gac tac acc gtt ctc ggt gca gat ggc 739Ile Arg Glu Trp Val Pro Gly Asp Tyr Thr Val Leu Gly Ala Asp Gly
200 205 210ttc ggt ttc tct gat acc cgc cca gct gct cgt cgc ttc ttc aac atc 787Phe Gly Phe Ser Asp Thr Arg Pro Ala Ala Arg Arg Phe Phe Asn Ile
215 220 225gac gct gag tcc att gtt gtt gca gtg ctg aac tcc ctg gca cgc gaa 835Asp Ala Glu Ser Ile Val Val Ala Val Leu Asn Ser Leu Ala Arg Glu230 235 240 245ggc aag atc gac gtc tcc gtt gct gct cag gct gct gag aag ttc aag 883Gly Lys Ile Asp Val Ser Val Ala Ala Gln Ala Ala Glu Lys Phe Lys
250 255 260ttg gat gat cct acg agt gtt tcc gta gat cca aac gct cct gag gaa 931Leu Asp Asp Pro Thr Ser Val Ser Val Asp Pro Asn Ala Pro Glu Glu
265 270 275taaatcacct caagggacag ata 954<210>96<211>277<212>PRT<213>谷氨酸棒杆菌<400>96Met Ala Arg Gly Phe Phe Leu Gly Ala Thr Ala Gly Arg Thr Thr Leu1 5 10 15Thr Gly Glu Gly Leu Gln His Met Asp Gly His Ser Pro Val Leu Ala
20 25 30Ser Thr Asn Glu Gly Val Glu Thr Tyr Asp Pro Ser Phe Ala Tyr Glu
35 40 45Ile Ala His Leu Val His Arg Gly Ile Asp Arg Met Tyr Gly Pro Gly
50 55 60Lys Gly Glu Asp Val Ile Tyr Tyr Ile Thr Ile Tyr Asn Glu Pro Thr65 70 75 80Pro Gln Pro Ala Glu Pro Glu Gly Leu Asp Val Glu Gly Leu His Lys
85 90 95Gly Ile Tyr Leu Tyr Ser Arg Gly Glu Gly Thr Gly His Glu Ala Asn
100 105 110Ile Leu Ala Ser Gly Val Gly Met Gln Trp Ala Leu Lys Ala Ala Ser
115 120 125Ile Leu Glu Ala Asp Tyr Gly Val Arg Ala Asn Ile Tyr Ser Ala Thr
130 135 140Ser Trp Val Asn Leu Ala Arg Asp Gly Ala Ala Arg Asn Lys Ala Gln145 150 155 160Leu Arg Asn Pro Gly Ala Asp Ala Gly Glu Ala Phe Val Thr Thr Gln
165 170 175Leu Lys Gln Thr Ser Gly Pro Tyr Val Ala Val Ser Asp Phe Ser Thr
180 185 190Asp Leu Pro Asn Gln Ile Arg Glu Trp Val Pro Gly Asp Tyr Thr Val
195 200 205Leu Gly Ala Asp Gly Phe Gly Phe Ser Asp Thr Arg Pro Ala Ala Arg
210 215 220Arg Phe Phe Asn Ile Asp Ala Glu Ser Ile Val Val Ala Val Leu Asn225 230 235 240Ser Leu Ala Arg Glu Gly Lys Ile Asp Val Ser Val Ala Ala Gln Ala
245 250 255Ala Glu Lys Phe Lys Leu Asp Asp Pro Thr Ser Val Ser Val Asp Pro
260 265 270Asn Ala Pro Glu Glu
275<210>97<211>508<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(508)<223>RXN03086<400>97ttcgtgcact tcggcgtgtc acaattaggt acgaccaaga atgggaccgg gaaaccggga 60cgtataaacg aaataaaaca ttccaacagg aggtgtggaa atg gcc gat caa gca 115
Met Ala Asp Gln Ala
1 5aaa ctt ggt ggc aag ccc tcg gat gac tct aac ttc gcg atg atc cgc 163Lys Leu Gly Gly Lys Pro Ser Asp Asp Ser Asn Phe Ala Met Ile Arg
10 15 20gat ggc gtg gca tct tat ttg aac gac tca gat ccg gag gag acc aac 211Asp Gly Val Ala Ser Tyr Leu Asn Asp Ser Asp Pro Glu Glu Thr Asn
25 30 35gag tgg atg gat tca ctc gac gga tta ctc cag gag tct tct cca gaa 259Glu Trp Met Asp Ser Leu Asp Gly Leu Leu Gln Glu Ser Ser Pro Glu
40 45 50cgt gct cgt tac ctc atg ctt cgt ttg ctt gag cgt gca tct gca aag 307Arg Ala Arg Tyr Leu Met Leu Arg Leu Leu Glu Arg Ala Ser Ala Lys
55 60 65cgc gta tct ctt ccc cca atg acg tca acc gac tac gtc aac acc att 355Arg Val Ser Leu Pro Pro Met Thr Ser Thr Asp Tyr Val Asn Thr Ile70 75 80 85cca acc tct atg gaa cct gaa ttc cca ggc gat gag gaa atg gag aag 403Pro Thr Ser Met Glu Pro Glu Phe Pro Gly Asp Glu Glu Met Glu Lys
90 95 100cgt tac cgt cgt tgg att cgc tgg aac gca gcc atc atg gtt cac cgc 451Arg Tyr Arg Arg Trp Ile Arg Trp Asn Ala Ala Ile Met Val His Arg
105 110 115gct cag cga cca ggc atc ggc gtc ggc gga cac att tcc act tac gca 499Ala Gln Arg Pro Gly Ile Gly Val Gly Gly His Ile Ser Thr Tyr Ala
120 125 130ggc gca gcc 508Gly Ala Ala
135<210>98<211>136<212>PRT<213>谷氨酸棒杆菌<400>98Met Ala Asp Gln Ala Lys Leu Gly Gly Lys Pro Ser Asp Asp Ser Asn1 5 10 15Phe Ala Met Ile Arg Asp Gly Val Ala Ser Tyr Leu Asn Asp Ser Asp
20 25 30Pro Glu Glu Thr Asn Glu Trp Met Asp Ser Leu Asp Gly Leu Leu Gln
35 40 45Glu Ser Ser Pro Glu Arg Ala Arg Tyr Leu Met Leu Arg Leu Leu Glu
50 55 60Arg Ala Ser Ala Lys Arg Val Ser Leu Pro Pro Met Thr Ser Thr Asp65 70 75 80Tyr Val Asn Thr Ile Pro Thr Ser Met Glu Pro Glu Phe Pro Gly Asp
85 90 95Glu Glu Met Glu Lys Arg Tyr Arg Arg Trp Ile Arg Trp Asn Ala Ala
100 105 110Ile Met Val His Arg Ala Gln Arg Pro Gly Ile Gly Val Gly Gly His
115 120 125Ile Ser Thr Tyr Ala Gly Ala Ala
130 135<210>99<211>508<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(508)<223>FRXA02887<400>99ttcgtgcact tcggcgtgcc acaattaggt acgaccaaga atgggaccgg gaaaccggga 60cgtataaacg aaataaaaca ttccaacagg aggtgtggaa atg gcc gat caa gca 115
Met Ala Asp Gln Ala
1 5aaa ctt ggt ggc aag ccc tcg gat gac tct aac ttc gcg atg atc cgc 163Lys Leu Gly Gly Lys Pro Ser Asp Asp Ser Asn Phe Ala Met Ile Arg
10 15 20gat ggc gtg gca tct tat ttg aac gac tca gat ccg gag gag acc aac 211Asp Gly Val Ala Ser Tyr Leu Asn Asp Ser Asp Pro Glu Glu Thr Asn
25 30 35gag tgg atg gat tca ctc gac gga tta ctc cag gag tct tct cca gaa 259Glu Trp Met Asp Ser Leu Asp Gly Leu Leu Gln Glu Ser Ser Pro Glu
40 45 50cgt gct cgt tac ctc atg ctt cgt ttg ctt gag cgt gca tct gca aag 307Arg Ala Arg Tyr Leu Met Leu Arg Leu Leu Glu Arg Ala Ser Ala Lys
55 60 65cgc gta tct ctt ccc cca atg acg tca acc gac tac gtc aac acc att 355Arg Val Ser Leu Pro Pro Met Thr Ser Thr Asp Tyr Val Asn Thr Ile70 75 80 85cca acc tct atg gaa cct gaa ttc cca ggc gat gag gaa atg gag aag 403Pro Thr Ser Met Glu Pro Glu Phe Pro Gly Asp Glu Glu Met Glu Lys
90 95 100cgt tac cgt cgt tgg att cgc tgg aac gca gcc atc atg gtt cac cgc 451Arg Tyr Arg Arg Trp Ile Arg Trp Asn Ala Ala Ile Met Val His Arg
105 110 115gct cag cga cca ggc atc ggc gtc ggc gga cac att tcc act tac gca 499Ala Gln Arg Pro Gly Ile Gly Val Gly Gly His Ile Ser Thr Tyr Ala
120 125 130ggc gca gcc 508Gly Ala Ala
135<210>100<211>136<212>PRT<213>谷氨酸棒杆菌<400>100Met Ala Asp Gln Ala Lys Leu Gly Gly Lys Pro Ser Asp Asp Ser Asn1 5 10 15Phe Ala Met Ile Arg Asp Gly Val Ala Ser Tyr Leu Asn Asp Ser Asp
20 25 30Pro Glu Glu Thr Asn Glu Trp Met Asp Ser Leu Asp Gly Leu Leu Gln
35 40 45Glu Ser Ser Pro Glu Arg Ala Arg Tyr Leu Met Leu Arg Leu Leu Glu
50 55 60Arg Ala Ser Ala Lys Arg Val Ser Leu Pro Pro Met Thr Ser Thr Asp65 70 75 80Tyr Val Asn Thr Ile Pro Thr Ser Met Glu Pro Glu Phe Pro Gly Asp
85 90 95Glu Glu Met Glu Lys Arg Tyr Arg Arg Trp Ile Arg Trp Asn Ala Ala
100 105 110Ile Met Val His Arg Ala Gln Arg Pro Gly Ile Gly Val Gly Gly His
115 120 125Ile Ser Thr Tyr Ala Gly Ala Ala
130 135<210>101<211>1385<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1362)<223>RXN03043<400>101gat ctc gat ggc ttc cgt cag gaa gtt tcc cgt gag cag ggt ggc att 48Asp Leu Asp Gly Phe Arg Gln Glu Val Ser Arg Glu Gln Gly Gly Ile1 5 10 15ccg tcc tac cct cac cca cac ggt atg aag gac ttc tgg gag ttc cca 96Pro Ser Tyr Pro His Pro His Gly Met Lys Asp Phe Trp Glu Phe Pro
20 25 30act gtg tcc atg ggt ctt ggc cca atg gat gcc att tac cag gca cgt 144Thr Val Ser Met Gly Leu Gly Pro Met Asp Ala Ile Tyr Gln Ala Arg
35 40 45ttc aac cgc tac ctc gaa aac cgt ggc atc aag gac acc tct gac cag 192Phe Asn Arg Tyr Leu Glu Asn Arg Gly Ile Lys Asp Thr Ser Asp Gln
50 55 60cac gtc tgg gcc ttc ctt ggc gac ggc gaa atg gac gag cca gaa tca 240His Val Trp Ala Phe Leu Gly Asp Gly Glu Met Asp Glu Pro Glu Ser65 70 75 80cgt ggt ctc atc cag cag gct gca ctg aac aac ctg gac aac ctg acc 288Arg Gly Leu Ile Gln Gln Ala Ala Leu Asn Asn Leu Asp Asn Leu Thr
85 90 95ttc gtg gtt aac tgc aac ctg cag cgt ctc gac gga cct gtc cgc ggt 336Phe Val Val Asn Cys Asn Leu Gln Arg Leu Asp Gly Pro Val Arg Gly
100 105 110aac acc aag atc atc cag gaa ctc gag tcc ttc ttc cgt ggc gca ggc 384Asn Thr Lys Ile Ile Gln Glu Leu Glu Ser Phe Phe Arg Gly Ala Gly
115 120 125tgg tct gtg atc aag gtt gtt tgg ggt cgc gag tgg gat gaa ctt ctg 432Trp Ser Val Ile Lys Val Val Trp Gly Arg Glu Trp Asp Glu Leu Leu
130 135 140gag aag gac cag gat ggt gca ctt gtt gag atc atg aac aac acc tcc 480Glu Lys Asp Gln Asp Gly Ala Leu Val Glu Ile Met Asn Asn Thr Ser145 150 155 160gat ggt gac tac cag acc ttc aag gct aac gac ggc gca tat gtt cgt 528Asp Gly Asp Tyr Gln Thr Phe Lys Ala Asn Asp Gly Ala Tyr Val Arg
165 170 175gag cac ttc ttc gga cgt gac cca cgc acc gca aag ctc gtt gag aac 576Glu His Phe Phe Gly Arg Asp Pro Arg Thr Ala Lys Leu Val Glu Asn
180 185 190atg acc gac gaa gaa atc tgg aag ctt cca cgt ggc ggc cac gat tac 624Met Thr Asp Glu Glu Ile Trp Lys Leu Pro Arg Gly Gly His Asp Tyr
195 200 205cgc aag gtt tac gca gcc tac aag cga gct ctt gag acc aag gat cgc 672Arg Lys Val Tyr Ala Ala Tyr Lys Arg Ala Leu Glu Thr Lys Asp Arg
210 215 220cca acc gtc atc ctt gct cac acc att aag ggc tac gga ctc ggc cac 720Pro Thr Val Ile Leu Ala His Thr Ile Lys Gly Tyr Gly Leu Gly His225 230 235 240aac ttc gaa ggc cgt aac gca acc cac cag atg aag aag ctg acg ctt 768Asn Phe Glu Gly Arg Asn Ala Thr His Gln Met Lys Lys Leu Thr Leu
245 250 255gat gat ctg aag ttg ttc cgc gac aag cag ggc atc cca atc acc gat 816Asp Asp Leu Lys Leu Phe Arg Asp Lys Gln Gly Ile Pro Ile Thr Asp
260 265 270gag cag ctg gag aag gat cct tac ctt cct cct tac tac cac cca ggt 864Glu Gln Leu Glu Lys Asp Pro Tyr Leu Pro Pro Tyr Tyr His Pro Gly
275 280 285gaa gac gct cct gaa atc aag tac atg aag gaa cgt cgc gca gcg ctc 912Glu Asp Ala Pro Glu Ile Lys Tyr Met Lys Glu Arg Arg Ala Ala Leu
290 295 300ggt ggc tac ctg cca gag cgt cgt gag aac tac gat cca att cag gtt 960Gly Gly Tyr Leu Pro Glu Arg Arg Glu Asn Tyr Asp Pro Ile Gln Val305 310 315 320cca cca ctg gat aag ctt cgc tct gtc cgt aag ggc tcc ggc aag cag 1008Pro Pro Leu Asp Lys Leu Arg Ser Val Arg Lys Gly Ser Gly Lys Gln
325 330 335cag atc gct acc acc atg gcg act gtt cgt acc ttc aag gaa ctg atg 1056Gln Ile Ala Thr Thr Met Ala Thr Val Arg Thr Phe Lys Glu Leu Met
340 345 350cgc gat aag ggc ttg gct gat cgc ctt gtc cca atc att cct gat gag 1104Arg Asp Lys Gly Leu Ala Asp Arg Leu Val Pro Ile Ile Pro Asp Glu
355 360 365gca cgt acc ttc ggt ctt gac tct tgg ttc cca acc ttg aag atc tac 1152Ala Arg Thr Phe Gly Leu Asp Ser Trp Phe Pro Thr Leu Lys Ile Tyr
370 375 380aac ccg cac ggt cag aac tac gtg cct gtt gac cac gac ctg atg ctc 1200Asn Pro His Gly Gln Asn Tyr Val Pro Val Asp His Asp Leu Met Leu385 390 395 400tcc tac cgt gag gca cct gaa gga cag atc ctg cac gaa ggc atc aac 1248Ser Tyr Arg Glu Ala Pro Glu Gly Gln Ile Leu His Glu Gly Ile Asn
405 410 415gag gct ggt tcc gtg gca tcg ttc atc gct gcg ggt acc tcc tac gcc 1296Glu Ala Gly Ser Val Ala Ser Phe Ile Ala Ala Gly Thr Ser Tyr Ala
420 425 430acc cac ggc aag gcc atg att ccg ctg tac atc ttc tac tcg atg ttc 1344Thr His Gly Lys Ala Met Ile Pro Leu Tyr Ile Phe Tyr Ser Met Phe
435 440 445ggg att cca gcg cac cgg tgactccatc tgggcagcag ccg 1385Gly Ile Pro Ala His Arg
450<210>102<211>454<212>PRT<213>谷氨酸棒杆菌<400>102Asp Leu Asp Gly Phe Arg Gln Glu Val Ser Arg Glu Gln Gly Gly Ile1 5 10 15Pro Ser Tyr Pro His Pro His Gly Met Lys Asp Phe Trp Glu Phe Pro
20 25 30Thr Val Ser Met Gly Leu Gly Pro Met Asp Ala Ile Tyr Gln Ala Arg
35 40 45Phe Asn Arg Tyr Leu Glu Asn Arg Gly Ile Lys Asp Thr Ser Asp Gln
50 55 60His Val Trp Ala Phe Leu Gly Asp Gly Glu Met Asp Glu Pro Glu Ser65 70 75 80Arg Gly Leu Ile Gln Gln Ala Ala Leu Asn Asn Leu Asp Asn Leu Thr
85 90 95Phe Val Val Asn Cys Asn Leu Gln Arg Leu Asp Gly Pro Val Arg Gly
100 105 110Asn Thr Lys Ile Ile Gln Glu Leu Glu Ser Phe Phe Arg Gly Ala Gly
115 120 125Trp Ser Val Ile Lys Val Val Trp Gly Arg Glu Trp Asp Glu Leu Leu
130 135 140Glu Lys Asp Gln Asp Gly Ala Leu Val Glu Ile Met Asn Asn Thr Ser145 150 155 160Asp Gly Asp Tyr Gln Thr Phe Lys Ala Asn Asp Gly Ala Tyr Val Arg
165 170 175Glu His Phe Phe Gly Arg Asp Pro Arg Thr Ala Lys Leu Val Glu Asn
180 185 190Met Thr Asp Glu Glu Ile Trp Lys Leu Pro Arg Gly Gly His Asp Tyr
195 200 205Arg Lys Val Tyr Ala Ala Tyr Lys Arg Ala Leu Glu Thr Lys Asp Arg
210 215 220Pro Thr Val Ile Leu Ala His Thr Ile Lys Gly Tyr Gly Leu Gly His225 230 235 240Asn Phe Glu Gly Arg Asn Ala Thr His Gln Met Lys Lys Leu Thr Leu
245 250 255Asp Asp Leu Lys Leu Phe Arg Asp Lys Gln Gly Ile Pro Ile Thr Asp
260 265 270Glu Gln Leu Glu Lys Asp Pro Tyr Leu Pro Pro Tyr Tyr His Pro Gly
275 280 285Glu Asp Ala Pro Glu Ile Lys Tyr Met Lys Glu Arg Arg Ala Ala Leu
290 295 300Gly Gly Tyr Leu Pro Glu Arg Arg Glu Asn Tyr Asp Pro Ile Gln Val305 310 315 320Pro Pro Leu Asp Lys Leu Arg Ser Val Arg Lys Gly Ser Gly Lys Gln
325 330 335Gln Ile Ala Thr Thr Met Ala Thr Val Arg Thr Phe Lys Glu Leu Met
340 345 350Arg Asp Lys Gly Leu Ala Asp Arg Leu Val Pro Ile Ile Pro Asp Glu
355 360 365Ala Arg Thr Phe Gly Leu Asp Ser Trp Phe Pro Thr Leu Lys Ile Tyr
370 375 380Asn Pro His Gly Gln Asn Tyr Val Pro Val Asp His Asp Leu Met Leu385 390 395 400Ser Tyr Arg Glu Ala Pro Glu Gly Gln Ile Leu His Glu Gly Ile Asn
405 410 415Glu Ala Gly Ser Val Ala Ser Phe Ile Ala Ala Gly Thr Ser Tyr Ala
420 425 430Thr His Gly Lys Ala Met Ile Pro Leu Tyr Ile Phe Tyr Ser Met Phe
435 440 445Gly Ile Pro Ala His Arg
450<210>103<211>1287<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1287)<223>FRXA02897<400>103gat ctc gat ggc ttc cgt cag gaa gtt tcc cgt gag cag ggt ggc att 48Asp Leu Asp Gly Phe Arg Gln Glu Val Ser Arg Glu Gln Gly Gly Ile1 5 10 15ccg tcc tac cct cac cca cac ggt atg aag gac ttc tgg gag ttc cca 96Pro Ser Tyr Pro His Pro His Gly Met Lys Asp Phe Trp Glu Phe Pro
20 25 30act gtg tcc atg ggt ctt ggc cca atg gat gcc att tac cag gca cgt 144Thr Val Ser Met Gly Leu Gly Pro Met Asp Ala Ile Tyr Gln Ala Arg
35 40 45ttc aac cgc tac ctc gaa aac cgt ggc atc aag gac acc tct gac cag 192Phe Asn Arg Tyr Leu Glu Asn Arg Gly Ile Lys Asp Thr Ser Asp Gln
50 55 60cac gtc tgg gcc ttc ctt ggc gac ggc gaa atg gac gag cca gaa tca 240His Val Trp Ala Phe Leu Gly Asp Gly Glu Met Asp Glu Pro Glu Ser65 70 75 80cgt ggt ctc atc cag cag gct gca ctg aac aac ctg gac aac ctg acc 288Arg Gly Leu Ile Gln Gln Ala Ala Leu Asn Asn Leu Asp Asn Leu Thr
85 90 95ttc gtg gtt aac tgc aac ctg cag cgt ctc gac gga cct gtc cgc ggt 336Phe Val Val Asn Cys Asn Leu Gln Arg Leu Asp Gly Pro Val Arg Gly
100 105 110aac acc aag atc atc cag gaa ctc gag tcc ttc ttc cgt ggc gca ggc 384Asn Thr Lys Ile Ile Gln Glu Leu Glu Ser Phe Phe Arg Gly Ala Gly
115 120 125tgg tct gtg atc aag gtt gtt tgg ggt cgc gag tgg gat gaa ctt ctg 432Trp Ser Val Ile Lys Val Val Trp Gly Arg Glu Trp Asp Glu Leu Leu
130 135 140gag aag gac cag gat ggt gca ctt gtt gag atc atg aac aac acc tcc 480Glu Lys Asp Gln Asp Gly Ala Leu Val Glu Ile Met Asn Asn Thr Ser145 150 155 160gat ggt gac tac cag acc ttc aag gct aac gac ggc gca tat gtt cgt 528Asp Gly Asp Tyr Gln Thr Phe Lys Ala Asn Asp Gly Ala Tyr Val Arg
165 170 175gag cac ttc ttc gga cgt gac cca cgc acc gca aag ctc gtt gag aac 576Glu His Phe Phe Gly Arg Asp Pro Arg Thr Ala Lys Leu Val Glu Asn
180 185 190atg acc gac gaa gaa atc tgg aag ctt cca cgt ggc ggc cac gat tac 624Met Thr Asp Glu Glu Ile Trp Lys Leu Pro Arg Gly Gly His Asp Tyr
195 200 205cgc aag gtt tac gca gcc tac aag cga gct ctt gag acc aag gat cgc 672Arg Lys Val Tyr Ala Ala Tyr Lys Arg Ala Leu Glu Thr Lys Asp Arg
210 215 220cca acc gtc atc ctt gct cac acc att aag ggc tac gga ctc ggc cac 720Pro Thr Val Ile Leu Ala His Thr Ile Lys Gly Tyr Gly Leu Gly His225 230 235 240aac ttc gaa ggc cgt aac gca acc cac cag atg aag aag ctg acg ctt 768Asn Phe Glu Gly Arg Asn Ala Thr His Gln Met Lys Lys Leu Thr Leu
245 250 255gat gat ctg aag ttg ttc cgc gac aag cag ggc atc cca atc acc gat 816Asp Asp Leu Lys Leu Phe Arg Asp Lys Gln Gly Ile Pro Ile Thr Asp
260 265 270gag cag ctg gag aag gat cct tac ctt cct cct tac tac cac cca ggt 864Glu Gln Leu Glu Lys Asp Pro Tyr Leu Pro Pro Tyr Tyr His Pro Gly
275 280 285gaa gac gct cct gaa atc aag tac atg aag gaa cgt cgc gca gcg ctc 912Glu Asp Ala Pro Glu Ile Lys Tyr Met Lys Glu Arg Arg Ala Ala Leu
290 295 300ggt ggc tac ctg cca gag cgt cgt gag aac tac gat cca att cag gtt 960Gly Gly Tyr Leu Pro Glu Arg Arg Glu Asn Tyr Asp Pro Ile Gln Val305 310 315 320cca cca ctg gat aag ctt cgc tct gtc cgt aag ggc tcc ggc aag cag 1008Pro Pro Leu Asp Lys Leu Arg Ser Val Arg Lys Gly Ser Gly Lys Gln
325 330 335cag atc gct acc acc atg gcg act gtt cgt acc ttc aag gaa ctg atg 1056Gln Ile Ala Thr Thr Met Ala Thr Val Arg Thr Phe Lys Glu Leu Met
340 345 350cgc gat aag ggc ttg gct gat cgc ctt gtc cca atc att cct gat gag 1104Arg Asp Lys Gly Leu Ala Asp Arg Leu Val Pro Ile Ile Pro Asp Glu
355 360 365gca cgt acc ttc ggt ctt gac tct tgg ttc cca acc ttg aag atc tac 1152Ala Arg Thr Phe Gly Leu Asp Ser Trp Phe Pro Thr Leu Lys Ile Tyr
370 375 380aac ccg cac ggt cag aac tac gtg cct gtt gac cac gac ctg atg ctc 1200Asn Pro His Gly Gln Asn Tyr Val Pro Val Asp His Asp Leu Met Leu385 390 395 400tcc tac cgt gag gca cct gaa gga cag atc ctg cac gaa ggc atc aac 1248Ser Tyr Arg Glu Ala Pro Glu Gly Gln Ile Leu His Glu Gly Ile Asn
405 410 415gag gct ggt tcc gtg gca tcg ttc atc gct gcg ggt acc 1287Glu Ala Gly Ser Val Ala Ser Phe Ile Ala Ala Gly Thr
420 425<210>104<211>429<212>PRT<213>谷氨酸棒杆菌<400>104Asp Leu Asp Gly Phe Arg Gln Glu Val Ser Arg Glu Gln Gly Gly Ile1 5 10 15Pro Ser Tyr Pro His Pro His Gly Met Lys Asp Phe Trp Glu Phe Pro
20 25 30Thr Val Ser Met Gly Leu Gly Pro Met Asp Ala Ile Tyr Gln Ala Arg
35 40 45Phe Asn Arg Tyr Leu Glu Asn Arg Gly Ile Lys Asp Thr Ser Asp Gln
50 55 60His Val Trp Ala Phe Leu Gly Asp Gly Glu Met Asp Glu Pro Glu Ser65 70 75 80Arg Gly Leu Ile Gln Gln Ala Ala Leu Asn Asn Leu Asp Asn Leu Thr
85 90 95Phe Val Val Asn Cys Asn Leu Gln Arg Leu Asp Gly Pro Val Arg Gly
100 105 110Asn Thr Lys Ile Ile Gln Glu Leu Glu Ser Phe Phe Arg Gly Ala Gly
115 120 125Trp Ser Val Ile Lys Val Val Trp Gly Arg Glu Trp Asp Glu Leu Leu
130 135 140Glu Lys Asp Gln Asp Gly Ala Leu Val Glu Ile Met Asn Asn Thr Ser145 150 155 160Asp Gly Asp Tyr Gln Thr Phe Lys Ala Asn Asp Gly Ala Tyr Val Arg
165 170 175Glu His Phe Phe Gly Arg Asp Pro Arg Thr Ala Lys Leu Val Glu Asn
180 185 190Met Thr Asp Glu Glu Ile Trp Lys Leu Pro Arg Gly Gly His Asp Tyr
195 200 205Arg Lys Val Tyr Ala Ala Tyr Lys Arg Ala Leu Glu Thr Lys Asp Arg
210 215 220Pro Thr Val Ile Leu Ala His Thr Ile Lys Gly Tyr Gly Leu Gly His225 230 235 240Asn Phe Glu Gly Arg Asn Ala Thr His Gln Met Lys Lys Leu Thr Leu
245 250 255Asp Asp Leu Lys Leu Phe Arg Asp Lys Gln Gly Ile Pro Ile Thr Asp
260 265 270Glu Gln Leu Glu Lys Asp Pro Tyr Leu Pro Pro Tyr Tyr His Pro Gly
275 280 285Glu Asp Ala Pro Glu Ile Lys Tyr Met Lys Glu Arg Arg Ala Ala Leu
290 295 300Gly Gly Tyr Leu Pro Glu Arg Arg Glu Asn Tyr Asp Pro Ile Gln Val305 310 315 320Pro Pro Leu Asp Lys Leu Arg Ser Val Arg Lys Gly Ser Gly Lys Gln
325 330 335Gln Ile Ala Thr Thr Met Ala Thr Val Arg Thr Phe Lys Glu Leu Met
340 345 350Arg Asp Lys Gly Leu Ala Asp Arg Leu Val Pro Ile Ile Pro Asp Glu
355 360 365Ala Arg Thr Phe Gly Leu Asp Ser Trp Phe Pro Thr Leu Lys Ile Tyr
370 375 380Asn Pro His Gly Gln Asn Tyr Val Pro Val Asp His Asp Leu Met Leu385 390 395 400Ser Tyr Arg Glu Ala Pro Glu Gly Gln Ile Leu His Glu Gly Ile Asn
405 410 415Glu Ala Gly Ser Val Ala Ser Phe Ile Ala Ala Gly Thr
420 425<210>105<211>1133<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(88)..(1110)<223>RXN03083<400>105attcagcagt aatcatttag acttggaacc gcttaccagt ggtttcaaca atgcattcac 60ccagctcaca cgtgtggagg tgcctta atg gca aag agg atc gta att atc ggc 114
Met Ala Lys Arg Ile Val Ile Ile Gly
1 5ggt gga cct gca ggc tat gaa gcc gca ctc gca ggc gct aaa tac ggt 162Gly Gly Pro Ala Gly Tyr Glu Ala Ala Leu Ala Gly Ala Lys Tyr Gly10 15 20 25gca gaa gtt acc gtt att gaa gat gtc gga gtt ggc gga tcc gca gtc 210Ala Glu Val Thr Val Ile Glu Asp Val Gly Val Gly Gly Ser Ala Val
30 35 40acc atg gac tgt gta cct tca aag tcc ttc atc gct ggt acc ggt atc 258Thr Met Asp Cys Val Pro Ser Lys Ser Phe Ile Ala Gly Thr Gly Ile
45 50 55aaa acc gac ctc cga cgt gct gat gac atg gga ctt aac cgt ggg ctt 306Lys Thr Asp Leu Arg Arg Ala Asp Asp Met Gly Leu Asn Arg Gly Leu
60 65 70gga aaa gca cac cta gaa atc gat gca ctg aac atc cgt gtg aag gac 354Gly Lys Ala His Leu Glu Ile Asp Ala Leu Asn Ile Arg Val Lys Asp
75 80 85ctt gcg aaa gca cag tcc gaa gat atc ttg ggc cag ctg cag cgc tca 402Leu Ala Lys Ala Gln Ser Glu Asp Ile Leu Gly Gln Leu Gln Arg Ser90 95 100 105gat gtc cgc atg att aac ggt gtg ggc cgc ttt gat gat tac aac acc 450Asp Val Arg Met Ile Asn Gly Val Gly Arg Phe Asp Asp Tyr Asn Thr
110 115 120aag caa acc acc cac tac att aaa gtc acc cac agc gat ggc tcc gaa 498Lys Gln Thr Thr His Tyr Ile Lys Val Thr His Ser Asp Gly Ser Glu
125 130 135gaa acc gtt gag tgc gat ctg gtg ctg gtt gca act ggt gca acc ccc 546Glu Thr Val Glu Cys Asp Leu Val Leu Val Ala Thr Gly Ala Thr Pro
140 145 150cgc att ctt aaa ggt gca gag cca gac ggc gag cgc atc ttg acc tgg 594Arg Ile Leu Lys Gly Ala Glu Pro Asp Gly Glu Arg Ile Leu Thr Trp
155 160 165cgt cag gtc tac gac att gaa gaa ctc ccc acc cac ctt atc gtg gtt 642Arg Gln Val Tyr Asp Ile Glu Glu Leu Pro Thr His Leu Ile Val Val170 175 180 185ggt tcc ggt gtg acc ggt gcg gaa ttt gtc tct gcg ttt gct gaa ctc 690Gly Ser Gly Val Thr Gly Ala Glu Phe Val Ser Ala Phe Ala Glu Leu
190 195 200ggc gtc aaa gtc acc atg gtg gca tcc cgt gac cgc att ttg cct cac 738Gly Val Lys Val Thr Met Val Ala Ser Arg Asp Arg Ile Leu Pro His
205 210 215gat gac gca gat gcc gca gac gtg ctg gaa acc gtt ctg gct gag cgc 786Asp Asp Ala Asp Ala Ala Asp Val Leu Glu Thr Val Leu Ala Glu Arg
220 225 230gga gta tcc ctg gaa aag cat gcc cgc gtg gag tct gtc acc cgc acc 834Gly Val Ser Leu Glu Lys His Ala Arg Val Glu Sar Val Thr Arg Thr
235 240 245gaa gac ggt ggc gtg tgt gtt cgc act gct gac gga cga gaa atc tac 882Glu Asp Gly Gly Val Cys Val Arg Thr Ala Asp Gly Arg Glu Ile Tyr250 255 260 265ggt tct cac gcg ttg atg act gtt ggt tcc att cca aac acg gca gat 930Gly Ser His Ala Leu Met Thr Val Gly Ser Ile Pro Asn Thr Ala Asp
270 275 280ctt ggc ctg gag aac atc ggt gtt gag ctg gca cca tcc ggc cat atc 978Leu Gly Leu Glu Asn Ile Gly Val Glu Leu Ala Pro Ser Gly His Ile
285 290 295aag gtt gac cgg tct ccc gca cca aca tcc ccg gtg tgt acg cag cag 1026Lys Val Asp Arg Ser Pro Ala Pro Thr Ser Pro Val Cys Thr Gln Gln
300 305 310gtg act gta ctg acc tat tcc cac tgg cgt ccg ttg cag cga tgc agg 1074Val Thr Val Leu Thr Tyr Ser His Trp Arg Pro Leu Gln Arg Cys Arg
315 320 325gcc gta tcg cca tgt atc acg cac tcg gtg aag gcg tgagccccat 1120Ala Val Ser Pro Cys Ile Thr His Ser Val Lys Ala330 335 340ccgtttgaag act 1133<210>106<211>341<212>PRT<213>谷氨酸棒杆菌<400>106Met Ala Lys Arg Ile Val Ile Ile Gly Gly Gly Pro Ala Gly Tyr Glu1 5 10 15Ala Ala Leu Ala Gly Ala Lys Tyr Gly Ala Glu Val Thr Val Ile Glu
20 25 30Asp Val Gly Val Gly Gly Ser Ala Val Thr Met Asp Cys Val Pro Ser
35 40 45Lys Ser Phe Ile Ala Gly Thr Gly Ile Lys Thr Asp Leu Arg Arg Ala
50 55 60Asp Asp Met Gly Leu Asn Arg Gly Leu Gly Lys Ala His Leu Glu Ile65 70 75 80Asp Ala Leu Asn Ile Arg Val Lys Asp Leu Ala Lys Ala Gln Ser Glu
85 90 95Asp Ile Leu Gly Gln Leu Gln Arg Ser Asp Val Arg Met Ile Asn Gly
100 105 110Val Gly Arg Phe Asp Asp Tyr Asn Thr Lys Gln Thr Thr His Tyr Ile
115 120 125Lys Val Thr His Ser Asp Gly Ser Glu Glu Thr Val Glu Cys Asp Leu
130 135 140Val Leu Val Ala Thr Gly Ala Thr Pro Arg Ile Leu Lys Gly Ala Glu145 150 155 160Pro Asp Gly Glu Arg Ile Leu Thr Trp Arg Gln Val Tyr Asp Ile Glu
165 170 175Glu Leu Pro Thr His Leu Ile Val Val Gly Ser Gly Val Thr Gly Ala
180 185 190Glu Phe Val Ser Ala Phe Ala Glu Leu Gly Val Lys Val Thr Met Val
195 200 205Ala Ser Arg Asp Arg Ile Leu Pro His Asp Asp Ala Asp Ala Ala Asp
210 215 220Val Leu Glu Thr Val Leu Ala Glu Arg Gly Val Ser Leu Glu Lys His225 230 235 240Ala Arg Val Glu Ser Val Thr Arg Thr Glu Asp Gly Gly Val Cys Val
245 250 255Arg Thr Ala Asp Gly Arg Glu Ile Tyr Gly Ser His Ala Leu Met Thr
260 265 270Val Gly Ser Ile Pro Asn Thr Ala Asp Leu Gly Leu Glu Asn Ile Gly
275 280 285Val Glu Leu Ala Pro Ser Gly His Ile Lys Val Asp Arg Ser Pro Ala
290 295 300Pro Thr Ser Pro Val Cys Thr Gln Gln Val Thr Val Leu Thr Tyr Ser305 310 315 320His Trp Arg Pro Leu Gln Arg Cys Arg Ala Val Ser Pro Cys Ile Thr
325 330 335His Ser Val Lys Ala
340<210>107<211>1518<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(89)..(1495)<223>FRXA02853<400>107aattcagcag taatcattta gacttggaac cgcttaccag tggtttcaac aatgcattca 60cccagctcac acgtgtggag gtgcctta atg gca aag agg atc gta att atc ggc 115
Met Ala Lys Arg Ile Val Ile Ile Gly
1 5ggt gga cct gca ggc tat gaa gcc gca ctc gca ggc gct aaa tac ggt 163Gly Gly Pro Ala Gly Tyr Glu Ala Ala Leu Ala Gly Ala Lys Tyr Gly10 15 20 25gca gaa gtt acc gtt att gaa gat gtc gga gtt ggc gga tcc gca gtc 211Ala Glu Val Thr Val Ile Glu Asp Val Gly Val Gly Gly Ser Ala Val
30 35 40acc atg gac tgt gta cct tca aag tcc ttc atc gct ggt acc ggt atc 259Thr Met Asp Cys Val Pro Ser Lys Ser Phe Ile Ala Gly Thr Gly Ile
45 50 55aaa acc gac ctc cga cgt gct gat gac atg gga ctt aac cgt ggg ctt 307Lys Thr Asp Leu Arg Arg Ala Asp Asp Met Gly Leu Asn Arg Gly Leu
60 65 70gga aaa gca cac cta gaa atc gat gca ctg aac atc cgt gtg aag gac 355Gly Lys Ala His Leu Glu Ile Asp Ala Leu Asn Ile Arg Val Lys Asp
75 80 85ctt gcg aaa gca cag tcc gaa gat atc ttg ggc cag ctg cag cgc tca 403Leu Ala Lys Ala Gln Ser Glu Asp Ile Leu Gly Gln Leu Gln Arg Ser90 95 100 105gat gtc cgc atg att aac ggt gtg ggc cgc ttt gat gat tac aac acc 451Asp Val Arg Met Ile Asn Gly Val Gly Arg Phe Asp Asp Tyr Asn Thr
110 115 120aag caa acc acc cac tac att aaa gtc acc cac agc gat ggc tcc gaa 499Lys Gln Thr Thr His Tyr Ile Lys Val Thr His Ser Asp Gly Ser Glu
125 130 135gaa acc gtt gag tgc gat ctg gtg ctg gtt gca act ggt gca acc ccc 547Glu Thr Val Glu Cys Asp Leu Val Leu Val Ala Thr Gly Ala Thr Pro
140 145 150cgc att ctt aaa ggt gca gag cca gac ggc gag cgc atc ttg acc tgg 595Arg Ile Leu Lys Gly Ala Glu Pro Asp Gly Glu Arg Ile Leu Thr Trp
155 160 165cgt cag gtc tac gac att gaa gaa ctc ccc acc cac ctt atc gtg gtt 643Arg Gln Val Tyr Asp Ile Glu Glu Leu Pro Thr His Leu Ile Val Val170 175 180 185ggt tcc ggt gtg acc ggt gcg gaa ttt gtc tct gcg ttt gct gaa ctc 691Gly Ser Gly Val Thr Gly Ala Glu Phe Val Ser Ala Phe Ala Glu Leu
190 195 200ggc gtc aaa gtc acc atg gtg gca tcc cgt gac cgc att ttg cct cac 739Gly Val Lys Val Thr Met Val Ala Ser Arg Asp Arg Ile Leu Pro His
205 210 215gat gac gca gat gcc gca gac gtg ctg gaa acc gtt ctg gct gag cgc 787Asp Asp Ala Asp Ala Ala Asp Val Leu Glu Thr Val Leu Ala Glu Arg
220 225 230gga gta tcc ctg gaa aag cat gcc cgc gtg gag tct gtc acc cgc acc 835Gly Val Ser Leu Glu Lys His Ala Arg Val Glu Ser Val Thr Arg Thr
235 240 245gaa gac ggt ggc gtg tgt gtt cgc act gct gac gga cga gaa atc tac 883Glu Asp Gly Gly Val Cys Val Arg Thr Ala Asp Gly Arg Glu Ile Tyr250 255 260 265ggt tct cac gcg ttg atg act gtt ggt tcc att cca aac acg gca gat 931Gly Ser His Ala Leu Met Thr Val Gly Ser Ile Pro Asn Thr Ala Asp
270 275 280ctt ggc ctg gag aac atc ggt gtt gag ctg gca cca tcc ggc cat atc 979Leu Gly Leu Glu Asn Ile Gly Val Glu Leu Ala Pro Ser Gly His Ile
285 290 295aag gtt gac cgn gtc tcc cgc acc aac atc ccc ggt gtg tac gca gca 1027Lys Val Asp Arg Val Ser Arg Thr Asn Ile Pro Gly Val Tyr Ala Ala
300 305 310ggt gac tgt act gac cta ttc cca ctg gcg tcc gtt gca gcg atg cag 1075Gly Asp Cys Thr Asp Leu Phe Pro Leu Ala Ser Val Ala Ala Met Gln
315 320 325ggc cgt atc gcc atg tat cac gca ctc ggt gaa ggc gtg agc ccc atc 1123Gly Arg Ile Ala Met Tyr His Ala Leu Gly Glu Gly Val Ser Pro Ile330 335 340 345cgt ttg aag act gtt gcc acc gca gtg ttt acc cgc cca gag atc gca 1171Arg Leu Lys Thr Val Ala Thr Ala Val Phe Thr Arg Pro Glu Ile Ala
350 355 360gca gta ggt atc acc cat gca caa gtt gat tcc ggc gaa gtg tct gct 1219Ala Val Gly Ile Thr His Ala Gln Val Asp Ser Gly Glu Val Ser Ala
365 370 375cgc gtg att gtg ctt cct ttg gct act aac cca cgc gcc aag atg cgt 1267Arg Val Ile Val Leu Pro Leu Ala Thr Asn Pro Arg Ala Lys Met Arg
380 385 390tcc ctg cgc cac ggt ttt gtg aag ctg ttc tgc cgc cgt aac tct ggc 1315Ser Leu Arg His Gly Phe Val Lys Leu Phe Cys Arg Arg Asn Ser Gly
395 400 405ctg atc atc ggt ggt gtc gtg gtg gca ccg acc gcg tct gag ctg atc 1363Leu Ile Ile Gly Gly Val Val Val Ala Pro Thr Ala Ser Glu Leu Ile410 415 420 425cta ccg atc gct gtg gca gtg acc aac cgt ctg aca gtt gct gat ctg 1411Leu Pro Ile Ala Val Ala Val Thr Asn Arg Leu Thr Val Ala Asp Leu
430 435 440gct gat acc ttc gcg gtg tac cca tca ttg tca ggt tcg att act gaa 1459Ala Asp Thr Phe Ala Val Tyr Pro Ser Leu Ser Gly Ser Ile Thr Glu
445 450 455gca gca cgt cag ctg gtt caa cat gat gat cta ggc taatttttct 1505Ala Ala Arg Gln Leu Val Gln His Asp Asp Leu Gly
460 465gagtcttaga ttt 1518<210>108<211>469<212>PRT<213>谷氨酸棒杆菌<400>108Met Ala Lys Arg Ile Val Ile Ile Gly Gly Gly Pro Ala Gly Tyr Glu1 5 10 15Ala Ala Leu Ala Gly Ala Lys Tyr Gly Ala Glu Val Thr Val Ile Glu
20 25 30Asp Val Gly Val Gly Gly Ser Ala Val Thr Met Asp Cys Val Pro Ser
35 40 45Lys Ser Phe Ile Ala Gly Thr Gly Ile Lys Thr Asp Leu Arg Arg Ala
50 55 60Asp Asp Met Gly Leu Asn Arg Gly Leu Gly Lys Ala His Leu Glu Ile65 70 75 80Asp Ala Leu Asn Ile Arg Val Lys Asp Leu Ala Lys Ala Gln Ser Glu
85 90 95Asp Ile Leu Gly Gln Leu Gln Arg Ser Asp Val Arg Met Ile Asn Gly
100 105 110Val Gly Arg Phe Asp Asp Tyr Asn Thr Lys Gln Thr Thr His Tyr Ile
115 120 125Lys Val Thr His Ser Asp Gly Ser Glu Glu Thr Val Glu Cys Asp Leu
130 135 140Val Leu Val Ala Thr Gly Ala Thr Pro Arg Ile Leu Lys Gly Ala Glu145 150 155 160Pro Asp Gly Glu Arg Ile Leu Thr Trp Arg Gln Val Tyr Asp Ile Glu
165 170 175Glu Leu Pro Thr His Leu Ile Val Val Gly Ser Gly Val Thr Gly Ala
180 185 190Glu Phe Val Ser Ala Phe Ala Glu Leu Gly Val Lys Val Thr Met Val
195 200 205Ala Ser Arg Asp Arg Ile Leu Pro His Asp Asp Ala Asp Ala Ala Asp
210 215 220Val Leu Glu Thr Val Leu Ala Glu Arg Gly Val Ser Leu Glu Lys His225 230 235 240Ala Arg Val Glu Ser Val Thr Arg Thr Glu Asp Gly Gly Val Cys Val
245 250 255Arg Thr Ala Asp Gly Arg Glu Ile Tyr Gly Ser His Ala Leu Met Thr
260 265 270Val Gly Ser Ile Pro Asn Thr Ala Asp Leu Gly Leu Glu Asn Ile Gly
275 280 285Val Glu Leu Ala Pro Ser Gly His Ile Lys Val Asp Arg Val Ser Arg
290 295 300Thr Asn Ile Pro Gly Val Tyr Ala Ala Gly Asp Cys Thr Asp Leu Phe305 310 315 320Pro Leu Ala Ser Val Ala Ala Met Gln Gly Arg Ile Ala Met Tyr His
325 330 335Ala Leu Gly Glu Gly Val Ser Pro Ile Arg Leu Lys Thr Val Ala Thr
340 345 350Ala Val Phe Thr Arg Pro Glu Ile Ala Ala Val Gly Ile Thr His Ala
355 360 365Gln Val Asp Ser Gly Glu Val Ser Ala Arg Val Ile Val Leu Pro Leu
370 375 380Ala Thr Asn Pro Arg Ala Lys Met Arg Ser Leu Arg His Gly Phe Val385 390 395 400Lys Leu Phe Cys Arg Arg Asn Ser Gly Leu Ile Ile Gly Gly Val Val
405 410 415Val Ala Pro Thr Ala Ser Glu Leu Ile Leu Pro Ile Ala Val Ala Val
420 425 430Thr Asn Arg Leu Thr Val Ala Asp Leu Ala Asp Thr Phe Ala Val Tyr
435 440 445Pro Ser Leu Ser Gly Ser Ile Thr Glu Ala Ala Arg Gln Leu Val Gln
450 455 460His Asp Asp Leu Gly465<210>109<211>2895<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2872)<223>RXA02259<400>109atgagcccat gaaagccatc gaaatcaatc gcccagctaa acacctgttt tgctgggtga 60ttttttatct catgcacgcc aacaccctca atgtgaaaga gtg ttt aaa gta gtt 115
Val Phe Lys Val Val
1 5atg act gat ttt tta cgc gat gac atc agg ttc ctc ggt caa atc ctc 163Met Thr Asp Phe Leu Arg Asp Asp Ile Arg Phe Leu Gly Gln Ile Leu
10 15 20ggt gag gta att gcg gaa caa gaa ggc cag gag gtt tat gaa ctg gtc 211Gly Glu Val Ile Ala Glu Gln Glu Gly Gln Glu Val Tyr Glu Leu Val
25 30 35gaa caa gcg cgc ctg act tct ttt gat atc gcc aag ggc aac gcc gaa 259Glu Gln Ala Arg Leu Thr Ser Phe Asp Ile Ala Lys Gly Asn Ala Glu
40 45 50atg gat agc ctg gtt cag gtt ttc gac ggc att act cca gcc aag gca 307Met Asp Ser Leu Val Gln Val Phe Asp Gly Ile Thr Pro Ala Lys Ala
55 60 65aca ccg att gct cgc gca ttt tcc cac ttc gct ctg ctg gct aac ctg 355Thr Pro Ile Ala Arg Ala Phe Ser His Phe Ala Leu Leu Ala Asn Leu70 75 80 85gcg gaa gac ctc tac gat gaa gag ctt cgt gaa cag gct ctc gat gca 403Ala Glu Asp Leu Tyr Asp Glu Glu Leu Arg Glu Gln Ala Leu Asp Ala
90 95 100ggc gac acc cct ccg gac agc act ctt gat gcc acc tgg ctg aaa ctc 451Gly Asp Thr Pro Pro Asp Ser Thr Leu Asp Ala Thr Trp Leu Lys Leu
105 110 115aat gag ggc aat gtt ggc gca gaa gct gtg gcc gat gtg ctg cgc aat 499Asn Glu Gly Asn Val Gly Ala Glu Ala Val Ala Asp Val Leu Arg Asn
120 125 130gct gag gtg gcg ccg gtt ctg act gcg cac cca act gag act cgc cgc 547Ala Glu Val Ala Pro Val Leu Thr Ala His Pro Thr Glu Thr Arg Arg
135 140 145cgc act gtt ttt gat gcg caa aag tgg atc acc acc cac atg cgt gaa 595Arg Thr Val Phe Asp Ala Gln Lys Trp Ile Thr Thr His Met Arg Glu150 155 160 165cgc cac gct ttg cag tct gcg gag cct acc gct cgt acg caa agc aag 643Arg His Ala Leu Gln Ser Ala Glu Pro Thr Ala Arg Thr Gln Ser Lys
170 175 180ttg gat gag atc gag aag aac atc cgc cgt cgc atc acc att ttg tgg 691Leu Asp Glu Ile Glu Lys Asn Ile Arg Arg Arg Ile Thr Ile Leu Trp
185 190 195cag acc gcg ttg att cgt gtg gcc cgc cca cgt atc gag gac gag atc 739Gln Thr Ala Leu Ile Arg Val Ala Arg Pro Arg Ile Glu Asp Glu Ile
200 205 210gaa gta ggg ctg cgc tac tac aag ctg agc ctt ttg gaa gag att cca 787Glu Val Gly Leu Arg Tyr Tyr Lys Leu Ser Leu Leu Glu Glu Ile Pro
215 220 225cgt atc aac cgt gat gtg gct gtt gag ctt cgt gag cgt ttc ggc gag 835Arg Ile Asn Arg Asp Val Ala Val Glu Leu Arg Glu Arg Phe Gly Glu230 235 240 245ggt gtt cct ttg aag ccc gtg gtc aag cca ggt tcc tgg att ggt gga 883Gly Val Pro Leu Lys Pro Val Val Lys Pro Gly Ser Trp Ile Gly Gly
250 255 260gac cac gac ggt aac cct tat gtc acc gcg gaa aca gtt gag tat tcc 931Asp His Asp Gly Asn Pro Tyr Val Thr Ala Glu Thr Val Glu Tyr Ser
265 270 275act cac cgc gct gcg gaa acc gtg ctc aag tac tat gca cgc cag ctg 979Thr His Arg Ala Ala Glu Thr Val Leu Lys Tyr Tyr Ala Arg Gln Leu
280 285 290cat tcc ctc gag cat gag ctc agc ctg tcg gac cgc atg aat aag gtc 1027His Ser Leu Glu His Glu Leu Ser Leu Ser Asp Arg Met Asn Lys Val
295 300 305acc ccg cag ctg ctt gcg ctg gca gat gca ggg cac aac gac gtg cca 1075Thr Pro Gln Leu Leu Ala Leu Ala Asp Ala Gly His Asn Asp Val Pro310 315 320 325agc cgc gtg gat gag cct tat cga cgc gcc gtc cat ggc gtt cgc gga 1123Ser Arg Val Asp Glu Pro Tyr Arg Arg Ala Val His Gly Val Arg Gly
330 335 340cgt atc ctc gcg acg acg gcc gag ctg atc ggc gag gac gcc gtt gag 1171Arg Ile Leu Ala Thr Thr Ala Glu Leu Ile Gly Glu Asp Ala Val Glu
345 350 355ggc gtg tgg ttc aag gtc ttt act cca tac gca tct ccg gaa gaa ttc 1219Gly Val Trp Phe Lys Val Phe Thr Pro Tyr Ala Ser Pro Glu Glu Phe
360 365 370tta aac gat gcg ttg acc att gat cat tct ctg cgt gaa tcc aag gac 1267Leu Asn Asp Ala Leu Thr Ile Asp His Ser Leu Arg Glu Ser Lys Asp
375 380 385gtt ctc att gcc gat gat cgt ttg tct gtg ctg att tct gcc atc gag 1315Val Leu Ile Ala Asp Asp Arg Leu Ser Val Leu Ile Ser Ala Ile Glu390 395 400 405agc ttt gga ttc aac ctt tac gca ctg gat ctg cgc caa aac tcc gaa 1363Ser Phe Gly Phe Asn Leu Tyr Ala Leu Asp Leu Arg Gln Asn Ser Glu
410 415 420agc tac gag gac gtc ctc acc gag ctt ttc gaa cgc gcc caa gtc acc 1411Ser Tyr Glu Asp Val Leu Thr Glu Leu Phe Glu Arg Ala Gln Val Thr
425 430 435gca aac tac cgc gag ctg tct gaa gca gag aag ctt gag gtg ctg ctg 1459Ala Asn Tyr Arg Glu Leu Ser Glu Ala Glu Lys Leu Glu Val Leu Leu
440 445 450aag gaa ctg cgc agc cct cgt ccg ctg atc ccg cac ggt tca gat gaa 1507Lys Glu Leu Arg Ser Pro Arg Pro Leu Ile Pro His Gly Ser Asp Glu
455 460 465tac agc gag gtc acc gac cgc gag ctc ggc atc ttc cgc acc gcg tcg 1555Tyr Ser Glu Val Thr Asp Arg Glu Leu Gly Ile Phe Arg Thr Ala Ser470 475 480 485gag gct gtt aag aaa ttc ggg cca cgg atg gtg cct cac tgc atc atc 1603Glu Ala Val Lys Lys Phe Gly Pro Arg Met Val Pro His Cys Ile Ile
490 495 500tcc atg gca tca tcg gtc acc gat gtg ctc gag ccg atg gtg ttg ctc 1651Ser Met Ala Ser Ser Val Thr Asp Val Leu Glu Pro Met Val Leu Leu
505 510 515aag gaa ttc gga ctc atc gca gcc aac ggc gac aac cca cgc ggc acc 1699Lys Glu Phe Gly Leu Ile Ala Ala Asn Gly Asp Asn Pro Arg Gly Thr
520 525 530gtc gat gtc atc cca ctg ttc gaa acc atc gaa gat ctc cag gcc ggc 1747Val Asp Val Ile Pro Leu Phe Glu Thr Ile Glu Asp Leu Gln Ala Gly
535 540 545gcc gga atc ctc gac gaa ctg tgg aaa att gat ctc tac cgc aac tac 1795Ala Gly Ile Leu Asp Glu Leu Trp Lys Ile Asp Leu Tyr Arg Asn Tyr550 555 560 565ctc ctg cag cgc gac aac gtc cag gaa gtc atg ctc ggt tac tcc gat 1843Leu Leu Gln Arg Asp Asn Val Gln Glu Val Met Leu Gly Tyr Ser Asp
570 575 580tcc aac aag gat ggc gga tat ttc tcc gca aac tgg gcg ctt tac gac 1891Ser Asn Lys Asp Gly Gly Tyr Phe Ser Ala Asn Trp Ala Leu Tyr Asp
585 590 595gcg gaa ctg cag ctc gtc gaa cta tgc cga tca gcc ggg gtc aag ctt 1939Ala Glu Leu Gln Leu Val Glu Leu Cys Arg Ser Ala Gly Val Lys Leu
600 605 610cgc ctg ttc cac ggc cgt ggt ggc acc gtc ggc cgc ggt ggc gga cct 1987Arg Leu Phe His Gly Arg Gly Gly Thr Val Gly Arg Gly Gly Gly Pro
615 620 625tcc tac gac gcg att ctt gcc cag ccc agg ggg gct gtc caa ggt tcc 2035Ser Tyr Asp Ala Ile Leu Ala Gln Pro Arg Gly Ala Val Gln Gly Ser630 635 640 645gtg cgc atc acc gag cag ggc gag atc atc tcc gct aag tac ggc aac 2083Val Arg Ile Thr Glu Gln Gly Glu Ile Ile Ser Ala Lys Tyr Gly Asn
650 655 660ccc gaa acc gcg cgc cga aac ctc gaa gcc ctg gtc tca gcc acg ctt 2131Pro Glu Thr Ala Arg Arg Asn Leu Glu Ala Leu Val Ser Ala Thr Leu
665 670 675gag gca tcg ctt ctc gac gtc tcc gaa ctc acc gat cac caa cgc gcg 2179Glu Ala Ser Leu Leu Asp Val Ser Glu Leu Thr Asp His Gln Arg Ala
680 685 690tac gac atc atg agt gag atc tct gag ctc agc ttg aag aag tac gcc 2227Tyr Asp Ile Met Ser Glu Ile Ser Glu Leu Ser Leu Lys Lys Tyr Ala
695 700 705tcc ttg gtg cac gag gat caa ggc ttc atc gat tac ttc acc cag tcc 2275Ser Leu Val His Glu Asp Gln Gly Phe Ile Asp Tyr Phe Thr Gln Ser710 715 720 725acg ccg ctg cag gag att gga tcc ctc aac atc gga tcc agg cct tcc 2323Thr Pro Leu Gln Glu Ile Gly Ser Leu Asn Ile Gly Ser Arg Pro Ser
730 735 740tca cgc aag cag acc tcc tcg gtg gaa gat ttg cga gcc atc cca tgg 2371Ser Arg Lys Gln Thr Ser Ser Val Glu Asp Leu Arg Ala Ile Pro Trp
745 750 755gtg ctc agc tgg tca cag tct cgt gtc atg ctg cca ggc tgg ttt ggt 2419Val Leu Ser Trp Ser Gln Ser Arg Val Met Leu Pro Gly Trp Phe Gly
760 765 770gtc gga acc gca tta gag cag tgg att ggc gaa ggg gag cag gcc acc 2467Val Gly Thr Ala Leu Glu Gln Trp Ile Gly Glu Gly Glu Gln Ala Thr
775 780 785caa cgc att gcc gag ctg caa aca ctc aat gag tcc tgg cca ttt ttc 2515Gln Arg Ile Ala Glu Leu Gln Thr Leu Asn Glu Ser Trp Pro Phe Phe790 795 800 805acc tca gtg ttg gat aac atg gct cag gtg atg tcc aag gca gag ctg 2563Thr Ser Val Leu Asp Asn Met Ala Gln Val Met Ser Lys Ala Glu Leu
810 815 820cgt ttg gca aag ctc tac gca gac ctg atc cca gat acg gaa gta gcc 2611Arg Leu Ala Lys Leu Tyr Ala Asp Leu Ile Pro Asp Thr Glu Val Ala
825 830 835gag cga gtc tat tcc gtc atc cgc gag gag tac ttc ctg acc aag aag 2659Glu Arg Val Tyr Ser Val Ile Arg Glu Glu Tyr Phe Leu Thr Lys Lys
840 845 850atg ttc tgc gta atc acc ggc tct gat gat ctg ctt gat gac aac cca 2707Met Phe Cys Val Ile Thr Gly Ser Asp Asp Leu Leu Asp Asp Asn Pro
855 860 865ctt ctc gca cgc tct gtc cag cgc cga tac ccc tac ctg ctt cca ctc 2755Leu Leu Ala Arg Ser Val Gln Arg Arg Tyr Pro Tyr Leu Leu Pro Leu870 875 880 885aac gtg atc cag gta gag atg atg cga cgc tac cga aaa ggc gac caa 2803Asn Val Ile Gln Val Glu Met Met Arg Arg Tyr Arg Lys Gly Asp Gln
890 895 900agc gag caa gtg tcc cgc aac att cag ctg acc atg aac ggt ctt tcc 2851Ser Glu Gln Val Ser Arg Asn Ile Gln Leu Thr Met Asn Gly Leu Ser
905 910 915act gcg ctg cgc aac tcc ggc tagtccagcc ggctgggtag tac 2895Thr Ala Leu Arg Asn Ser Gly
920<210>110<211>924<212>PRT<213>谷氨酸棒杆菌<400>110Val Phe Lys Val Val Met Thr Asp Phe Leu Arg Asp Asp Ile Arg Phe1 5 10 15Leu Gly Gln Ile Leu Gly Glu Val Ile Ala Glu Gln Glu Gly Gln Glu
20 25 30Val Tyr Glu Leu Val Glu Gln Ala Arg Leu Thr Ser Phe Asp Ile Ala
35 40 45Lys Gly Asn Ala Glu Met Asp Ser Leu Val Gln Val Phe Asp Gly Ile
50 55 60Thr Pro Ala Lys Ala Thr Pro Ile Ala Arg Ala Phe Ser His Phe Ala65 70 75 80Leu Leu Ala Asn Leu Ala Glu Asp Leu Tyr Asp Glu Glu Leu Arg Glu
85 90 95Gln Ala Leu Asp Ala Gly Asp Thr Pro Pro Asp Ser Thr Leu Asp Ala
100 105 110Thr Trp Leu Lys Leu Asn Glu Gly Asn Val Gly Ala Glu Ala Val Ala
115 120 125Asp Val Leu Arg Asn Ala Glu Val Ala Pro Val Leu Thr Ala His Pro
130 135 140Thr Glu Thr Arg Arg Arg Thr Val Phe Asp Ala Gln Lys Trp Ile Thr145 150 155 160Thr His Met Arg Glu Arg His Ala Leu Gln Ser Ala Glu Pro Thr Ala
165 170 175Arg Thr Gln Ser Lys Leu Asp Glu Ile Glu Lys Asn Ile Arg Arg Arg
180 185 190Ile Thr Ile Leu Trp Gln Thr Ala Leu Ile Arg Val Ala Arg Pro Arg
195 200 205Ile Glu Asp Glu Ile Glu Val Gly Leu Arg Tyr Tyr Lys Leu Ser Leu
210 215 220Leu Glu Glu Ile Pro Arg Ile Asn Arg Asp Val Ala Val Glu Leu Arg225 230 235 240Glu Arg Phe Gly Glu Gly Val Pro Leu Lys Pro Val Val Lys Pro Gly
245 250 255Ser Trp Ile Gly Gly Asp His Asp Gly Asn Pro Tyr Val Thr Ala Glu
260 265 270Thr Val Glu Tyr Ser Thr His Arg Ala Ala Glu Thr Val Leu Lys Tyr
275 280 285Tyr Ala Arg Gln Leu His Ser Leu Glu His Glu Leu Ser Leu Ser Asp
290 295 300Arg Met Asn Lys Val Thr Pro Gln Leu Leu Ala Leu Ala Asp Ala Gly305 310 315 320His Asn Asp Val Pro Ser Arg Val Asp Glu Pro Tyr Arg Arg Ala Val
325 330 335His Gly Val Arg Gly Arg Ile Leu Ala Thr Thr Ala Glu Leu Ile Gly
340 345 350Glu Asp Ala Val Glu Gly Val Trp Phe Lys Val Phe Thr Pro Tyr Ala
355 360 365Ser Pro Glu Glu Phe Leu Asn Asp Ala Leu Thr Ile Asp His Ser Leu
370 375 380Arg Glu Ser Lys Asp Val Leu Ile Ala Asp Asp Arg Leu Ser Val Leu385 390 395 400Ile Ser Ala Ile Glu Ser Phe Gly Phe Asn Leu Tyr Ala Leu Asp Leu
405 410 415Arg Gln Asn Ser Glu Ser Tyr Glu Asp Val Leu Thr Glu Leu Phe Glu
420 425 430Arg Ala Gln Val Thr Ala Asn Tyr Arg Glu Leu Ser Glu Ala Glu Lys
435 440 445Leu Glu Val Leu Leu Lys Glu Leu Arg Ser Pro Arg Pro Leu Ile Pro
450 455 460His Gly Ser Asp Glu Tyr Ser Glu Val Thr Asp Arg Glu Leu Gly Ile465 470 475 480Phe Arg Thr Ala Ser Glu Ala Val Lys Lys Phe Gly Pro Arg Met Val
485 490 495Pro His Cys Ile Ile Ser Met Ala Ser Ser Val Thr Asp Val Leu Glu
500 505 510Pro Met Val Leu Leu Lys Glu Phe Gly Leu Ile Ala Ala Asn Gly Asp
515 520 525Asn Pro Arg Gly Thr Val Asp Val Ile Pro Leu Phe Glu Thr Ile Glu
530 535 540Asp Leu Gln Ala Gly Ala Gly Ile Leu Asp Glu Leu Trp Lys Ile Asp545 550 555 560Leu Tyr Arg Asn Tyr Leu Leu Gln Arg Asp Asn Val Gln Glu Val Met
565 570 575Leu Gly Tyr Ser Asp Ser Asn Lys Asp Gly Gly Tyr Phe Ser Ala Asn
580 585 590Trp Ala Leu Tyr Asp Ala Glu Leu Gln Leu Val Glu Leu Cys Arg Ser
595 600 605Ala Gly Val Lys Leu Arg Leu Phe His Gly Arg Gly Gly Thr Val Gly
610 615 620Arg Gly Gly Gly Pro Ser Tyr Asp Ala Ile Leu Ala Gln Pro Arg Gly625 630 635 640Ala Val Gln Gly Ser Val Arg Ile Thr Glu Gln Gly Glu Ile Ile Ser
645 650 655Ala Lys Tyr Gly Asn Pro Glu Thr Ala Arg Arg Asn Leu Glu Ala Leu
660 665 670Val Ser Ala Thr Leu Glu Ala Ser Leu Leu Asp Val Ser Glu Leu Thr
675 680 685Asp His Gln Arg Ala Tyr Asp Ile Met Ser Glu Ile Ser Glu Leu Ser
690 695 700Leu Lys Lys Tyr Ala Ser Leu Val His Glu Asp Gln Gly Phe Ile Asp705 710 715 720Tyr Phe Thr Gln Ser Thr Pro Leu Gln Glu Ile Gly Ser Leu Asn Ile
725 730 735Gly Ser Arg Pro Ser Ser Arg Lys Gln Thr Ser Ser Val Glu Asp Leu
740 745 750Arg Ala Ile Pro Trp Val Leu Ser Trp Ser Gln Ser Arg Val Met Leu
755 760 765Pro Gly Trp Phe Gly Val Gly Thr Ala Leu Glu Gln Trp Ile Gly Glu
770 775 780Gly Glu Gln Ala Thr Gln Arg Ile Ala Glu Leu Gln Thr Leu Asn Glu785 790 795 800Ser Trp Pro Phe Phe Thr Ser Val Leu Asp Asn Met Ala Gln Val Met
805 810 815Ser Lys Ala Glu Leu Arg Leu Ala Lys Leu Tyr Ala Asp Leu Ile Pro
820 825 830Asp Thr Glu Val Ala Glu Arg Val Tyr Ser Val Ile Arg Glu Glu Tyr
835 840 845Phe Leu Thr Lys Lys Met Phe Cys Val Ile Thr Gly Ser Asp Asp Leu
850 855 860Leu Asp Asp Asn Pro Leu Leu Ala Arg Ser Val Gln Arg Arg Tyr Pro865 870 875 880Tyr Leu Leu Pro Leu Asn Val Ile Gln Val Glu Met Met Arg Arg Tyr
885 890 895Arg Lys Gly Asp Gln Ser Glu Gln Val Ser Arg Asn Ile Gln Leu Thr
900 905 910Met Asn Gly Leu Ser Thr Ala Leu Arg Asn Ser Gly
915 920<210>111<211>939<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(916)<223>RXN02326<400>111ccaggcggac agttgtccaa cctgcgtgca caggccaccg cactgggcct tgcggatcgt 60ttcgaactca tcgaagacaa ctacgcaagc cgttaatgag atg ctg gga cgc cca 115
Met Leu Gly Arg Pro
1 5acc aag gtc acc cca tcc tcc aag gtt gtt ggc gac ctc gca ctc cac 163Thr Lys Val Thr Pro Ser Ser Lys Val Val Gly Asp Leu Ala Leu His
10 15 20ctc gtt ggt gcg ggt gtg gat cca gca gac ttt gct gcc gat cca caa 211Leu Val Gly Ala Gly Val Asp Pro Ala Asp Phe Ala Ala Asp Pro Gln
25 30 35aag tac gac atc cca gac tct gtc atc gcg ttc ctg cgc ggc gag ctt 259Lys Tyr Asp Ile Pro Asp Ser Val Ile Ala Phe Leu Arg Gly Glu Leu
40 45 50ggt aac cct cca ggt ggc tgg cca gag cca ctg cgc acc cgc gca ctg 307Gly Asn Pro Pro Gly Gly Trp Pro Glu Pro Leu Arg Thr Arg Ala Leu
55 60 65gaa ggc cgc tcc gaa ggc aag gca cct ctg acg gaa gtt cct gag gaa 355Glu Gly Arg Ser Glu Gly Lys Ala Pro Leu Thr Glu Val Pro Glu Glu70 75 80 85gag cag gcg cac ctc gac gct gat gat tcc aag gaa cgt cgc aat agc 403Glu Gln Ala His Leu Asp Ala Asp Asp Ser Lys Glu Arg Arg Asn Ser
90 95 100ctc aac cgc ctg ctg ttc ccg aag cca acc gaa gag ttc ctc gag cac 451Leu Asn Arg Leu Leu Phe Pro Lys Pro Thr Glu Glu Phe Leu Glu His
105 110 115cgt cgc cgc ttc ggc aac acc tct gcg ctg gat gat cgt gaa ttc ttc 499Arg Arg Arg Phe Gly Asn Thr Ser Ala Leu Asp Asp Arg Glu Phe Phe
120 125 130tac ggc ctg gtc gaa ggc cgc gag act ttg atc cgc ctg cca gat gtg 547Tyr Gly Leu Val Glu Gly Arg Glu Thr Leu Ile Arg Leu Pro Asp Val
135 140 145cgc acc cca ctg ctt gtt cgc ctg gat gcg atc tct gag cca gac gat 595Arg Thr Pro Leu Lau Val Arg Leu Asp Ala Ile Ser Glu Pro Asp Asp150 155 160 165aag ggt atg cgc aat gtt gtg gcc aac gtc aac ggc cag atc cgc cca 643Lys Gly Met Arg Asn Val Val Ala Asn Val Asn Gly Gln Ile Arg Pro
170 175 180atg cgt gtg cgt gac cgc tcc gtt gag tct gtc acc gca acc gca gaa 691Met Arg Val Arg Asp Arg Ser Val Glu Ser Val Thr Ala Thr Ala Glu
185 190 195aag gca gat tcc tcc aac aag ggc cat gtt gct gca cca ttc gct ggt 739Lys Ala Asp Ser Ser Asn Lys Gly His Val Ala Ala Pro Phe Ala Gly
200 205 210gtt gtc acc gtg act gtt gct gaa ggt gat gag gtc aag gct gga gat 787Val Val Thr Val Thr Val Ala Glu Gly Asp Glu Val Lys Ala Gly Asp
215 220 225gca gtc gca atc atc gag gct atg aag atg gaa gca aca atc act gct 835Ala Val Ala Ile Ile Glu Ala Met Lys Met Glu Ala Thr Ile Thr Ala230 235 240 245tct gtt gac ggc aaa atc gat cgc gtt gtg gtt cct gct gca acg aag 883Ser Val Asp Gly Lys Ile Asp Arg Val Val Val Pro Ala Ala Thr Lys
250 255 260gtg gaa ggt ggc gac ttg atc gtc gtc gtt tcc taaacctttc tgtaaaaagc 936Val Glu Gly Gly Asp Leu Ile Val Val Val Ser
265 270ccc 939<210>112<211>272<212>PRT<213>谷氨酸棒杆菌<400>112Met Leu Gly Arg Pro Thr Lys Val Thr Pro Ser Ser Lys Val Val Gly1 5 10 15Asp Leu Ala Leu His Leu Val Gly Ala Gly Val Asp Pro Ala Asp Phe
20 25 30Ala Ala Asp Pro Gln Lys Tyr Asp Ile Pro Asp Ser Val Ile Ala Phe
35 40 45Leu Arg Gly Glu Leu Gly Asn Pro Pro Gly Gly Trp Pro Glu Pro Leu
50 55 60Arg Thr Arg Ala Leu Glu Gly Arg Ser Glu Gly Lys Ala Pro Leu Thr65 70 75 80Glu Val Pro Glu Glu Glu Gln Ala His Leu Asp Ala Asp Asp Ser Lys
85 90 95Glu Arg Arg Asn Ser Leu Asn Arg Leu Leu Phe Pro Lys Pro Thr Glu
100 105 110Glu Phe Leu Glu His Arg Arg Arg Phe Gly Asn Thr Ser Ala Leu Asp
115 120 125Asp Arg Glu Phe Phe Tyr Gly Leu Val Glu Gly Arg Glu Thr Leu Ile
130 135 140Arg Leu Pro Asp Val Arg Thr Pro Leu Leu Val Arg Leu Asp Ala Ile145 150 155 160Ser Glu Pro Asp Asp Lys Gly Met Arg Asn Val Val Ala Asn Val Asn
165 170 175Gly Gln Ile Arg Pro Met Arg Val Arg Asp Arg Ser Val Glu Ser Val
180 185 190Thr Ala Thr Ala Glu Lys Ala Asp Ser Ser Asn Lys Gly His Val Ala
195 200 205Ala Pro Phe Ala Gly Val Val Thr Val Thr Val Ala Glu Gly Asp Glu
210 215 220Val Lys Ala Gly Asp Ala Val Ala Ile Ile Glu Ala Met Lys Met Glu225 230 235 240Ala Thr Ile Thr Ala Ser Val Asp Gly Lys Ile Asp Arg Val Val Val
245 250 255Pro Ala Ala Thr Lys Val Glu Gly Gly Asp Leu Ile Val Val Val Ser
260 265 270<210>113<211>939<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(916)<223>FRXA02326<400>113ccaggcggac agttgtccaa cctgcgtgca caggccaccg cactgggcct tgcggatcgt 60ttcgaactca tcgaagacaa ctacgcaagc cgttaatgag atg ctg gga cgc cca 115
Met Leu Gly Arg Pro
1 5acc aag gtc acc cca tcc tcc aag gtt gtt ggc gac ctc gca ctc cac 163Thr Lys Val Thr Pro Ser Ser Lys Val Val Gly Asp Leu Ala Leu His
10 15 20ctc gtt ggt gcg ggt gtg gat cca gca gac ttt gct gcc gat cca caa 211Leu Val Gly Ala Gly Val Asp Pro Ala Asp Phe Ala Ala Asp Pro Gln
25 30 35aag tac gac atc cca gac tct gtc atc gcg ttc ctg cgc ggc gag ctt 259Lys Tyr Asp Ile Pro Asp Ser Val Ile Ala Phe Leu Arg Gly Glu Leu
40 45 50ggt aac cct cca ggt ggc tgg cca gag cca ctg cgc acc cgc gca ctg 307Gly Asn Pro Pro Gly Gly Trp Pro Glu Pro Leu Arg Thr Arg Ala Leu
55 60 65gaa ggc cgc tcc gaa ggc aag gca cct ctg acg gaa gtt cct gag gaa 355Glu Gly Arg Ser Glu Gly Lys Ala Pro Leu Thr Glu Val Pro Glu Glu70 75 80 85gag cag gcg cac ctc gac gct gat gat tcc aag gaa cgt cgc aat agc 403Glu Gln Ala His Leu Asp Ala Asp Asp Ser Lys Glu Arg Arg Asn Ser
90 95 100ctc aac cgc ctg ctg ttc ccg aag cca acc gaa gag ttc ctc gag cac 451Leu Asn Arg Leu Leu Phe Pro Lys Pro Thr Glu Glu Phe Leu Glu His
105 110 115cgt cgc cgc ttc ggc aac acc tct gcg ctg gat gat cgt gaa ttc ttc 499Arg Arg Arg Phe Gly Asn Thr Ser Ala Leu Asp Asp Arg Glu Phe Phe
120 125 130tac ggc ctg gtc gaa ggc cgc gag act ttg atc cgc ctg cca gat gtg 547Tyr Gly Leu Val Glu Gly Arg Glu Thr Leu Ile Arg Leu Pro Asp Val
135 140 145cgc acc cca ctg ctt gtt cgc ctg gat gcg atc tct gag cca gac gat 595Arg Thr Pro Leu Leu Val Arg Leu Asp Ala Ile Ser Glu Pro Asp Asp150 155 160 165aag ggt atg cgc aat gtt gtg gcc aac gtc aac ggc cag atc cgc cca 643Lys Gly Met Arg Asn Val Val Ala Asn Val Asn Gly Gln Ile Arg Pro
170 175 180atg cgt gtg cgt gac cgc tcc gtt gag tct gtc acc gca acc gca gaa 691Met Arg Val Arg Asp Arg Ser Val Glu Ser Val Thr Ala Thr Ala Glu
185 190 195aag gca gat tcc tcc aac aag ggc cat ggt gct gca cca ttc gct ggt 739Lys Ala Asp Ser Ser Asn Lys Gly His Val Ala Ala Pro Phe Ala Gly
200 205 210gtt gtc acc gtg act gtt gct gaa ggt gat gag gtc aag gct gga gat 787Val Val Thr Val Thr Val Ala Glu Gly Asp Glu Val Lys Ala Gly Asp
215 220 225gca gtc gca atc atc gag gct atg aag atg gaa gca aca atc act gct 835Ala Val Ala Ile Ile Glu Ala Met Lys Met Glu Ala Thr Ile Thr Ala230 235 240 245tct gtt gac ggc aaa atc gat cgc gtt gtg gtt cct gct gca acg aag 883Ser Val Asp Gly Lys Ile Asp Arg Val Val Val Pro Ala Ala Thr Lys
250 255 260gtg gaa ggt ggc gac ttg atc gtc gtc gtt tcc taaacctttc tgtaaaaagc 936Val Glu Gly Gly Asp Leu Ile Val Val Val Ser
265 270ccc 939<210>114<211>272<212>PRT<213>谷氨酸棒杆菌<400>114Met Leu Gly Arg Pro Thr Lys Val Thr Pro Ser Ser Lys Val Val Gly1 5 10 15Asp Leu Ala Leu His Leu Val Gly Ala Gly Val Asp Pro Ala Asp Phe
20 25 30Ala Ala Asp Pro Gln Lys Tyr Asp Ile Pro Asp Ser Val Ile Ala Phe
35 40 45Leu Arg Gly Glu Leu Gly Asn Pro Pro Gly Gly Trp Pro Glu Pro Leu
50 55 60Arg Thr Arg Ala Leu Glu Gly Arg Ser Glu Gly Lys Ala Pro Leu Thr65 70 75 80Glu Val Pro Glu Glu Glu Gln Ala His Leu Asp Ala Asp Asp Ser Lys
85 90 95Glu Arg Arg Asn Ser Leu Asn Arg Leu Leu Phe Pro Lys Pro Thr Glu
100 105 110Glu Phe Leu Glu His Arg Arg Arg Phe Gly Asn Thr Ser Ala Leu Asp
115 120 125Asp Arg Glu Phe Phe Tyr Gly Leu Val Glu Gly Arg Glu Thr Leu Ile
130 135 140Arg Leu Pro Asp Val Arg Thr Pro Leu Leu Val Arg Leu Asp Ala Ile145 150 155 160Ser Glu Pro Asp Asp Lys Gly Met Arg Asn Val Val Ala Asn Val Asn
165 170 175Gly Gln Ile Arg Pro Met Arg Val Arg Asp Arg Ser Val Glu Ser Val
180 185 190Thr Ala Thr Ala Glu Lys Ala Asp Ser Ser Asn Lys Gly His Val Ala
195 200 205Ala Pro Phe Ala Gly Val Val Thr Val Thr Val Ala Glu Gly Asp Glu
210 215 220Val Lys Ala Gly Asp Ala Val Ala Ile Ile Glu Ala Met Lys Met Glu225 230 235 240Ala Thr Ile Thr Ala Ser Val Asp Gly Lys Ile Asp Arg Val Val Val
245 250 255Pro Ala Ala Thr Lys Val Glu Gly Gly Asp Leu Ile Val Val Val Ser
260 265 270<210>115<211>1083<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1060)<223>RXN02327<400>115accgctgaag cagcttggcc cagccgcgtt tgctcgtgat ctccgtgagc aggacgcact 60ggcagttact gataccacct tccgcgatgc acaccagtct ttg ctt gcg acc cga 115
Leu Leu Ala Thr Arg
1 5gtc cgc tca ttc gca ctg aag cct gcg gca gag gcc gtc gca aag ctg 163Val Arg Ser Phe Ala Leu Lys Pro Ala Ala Glu Ala Val Ala Lys Leu
10 15 20act cct gag ctt ttg tcc gtg gag gcc tgg ggc ggc gcg acc tac gat 211Thr Pro Glu Leu Leu Ser Val Glu Ala Trp Gly Gly Ala Thr Tyr Asp
25 30 35gtg gcg atg cgt ttc ctc ttt gag gat ccg tgg gac agg ctc gac gag 259Val Ala Met Arg Phe Leu Phe Glu Asp Pro Trp Asp Arg Leu Asp Glu
40 45 50ctg cgc gag gcg atg ccg aat gta aac att cag atg ctg ctt cgc ggc 307Leu Arg Glu Ala Met Pro Asn Val Asn Ile Gln Met Leu Leu Arg Gly
55 60 65cgc aac acc gtg gga tac acc ccg tac cca gac tcc gtc tgc cgc gcg 355Arg Asn Thr Val Gly Tyr Thr Pro Tyr Pro Asp Ser Val Cys Arg Ala70 75 80 85ttt gtt aag gaa gct gcc agc tcc ggc gtg gac atc ttc cgc atc ttc 403Phe Val Lys Glu Ala Ala Ser Ser Gly Val Asp Ile Phe Arg Ile Phe
90 95 100gac gcg ctt aac gac gtc tcc cag atg cgt cca gca atc gac gca gtc 451Asp Ala Leu Asn Asp Val Ser Gln Met Arg Pro Ala Ile Asp Ala Val
105 110 115ctg gag acc aac acc gcg gta gcc gag gtg gct atg gct tat tct ggt 499Leu Glu Thr Asn Thr Ala Val Ala Glu Val Ala Met Ala Tyr Ser Gly
120 125 130gat ctc tct gat cca aat gaa aag ctc tac acc ctg gat tac tac cta 547Asp Leu Ser Asp Pro Asn Glu Lys Leu Tyr Thr Leu Asp Tyr Tyr Leu
135 140 145aag atg gca gag gag atc gtc aag tct ggc gct cac atc ttg gcc att 595Lys Met Ala Glu Glu Ile Val Lys Ser Gly Ala His Ile Leu Ala Ile150 155 160 165aag gat atg gct ggt ctg ctt cgc cca gct gcg gta acc aag ctg gtc 643Lys Asp Met Ala Gly Leu Leu Arg Pro Ala Ala Val Thr Lys Leu Val
170 175 180acc gca ctg cgc cgt gaa ttc gat ctg cca gtg cac gtg cac acc cac 691Thr Ala Leu Arg Arg Glu Phe Asp Leu Pro Val His Val His Thr His
185 190 195gac act gcg ggt ggc cag ctg gca acc tac ttt gct gca gct caa gct 739Asp Thr Ala Gly Gly Gln Leu Ala Thr Tyr Phe Ala Ala Ala Gln Ala
200 205 210ggt gca gat gct gtt gac ggt gct tcc ggc acc act gtc tgg cac cac 787Gly Ala Asp Ala Val Asp Gly Ala Ser Gly Thr Thr Val Trp His His
215 220 225ctc cca agc cat ccc ttg tct gcc att gtt gct gca ttc gcg cac acc 835Leu Pro Ser His Pro Leu Ser Ala Ile Val Ala Ala Phe Ala His Thr230 235 240 245cgt cgc gat acc ggt ttg agc ctc gag gct gtt tct gac ctc gag ccg 883Arg Arg Asp Thr Gly Leu Ser Leu Glu Ala Val Ser Asp Leu Glu Pro
250 255 260tac tgg gaa gca gtg cgc gga ctg tac ctg cca ttt gag tct gga acc 931Tyr Trp Glu Ala Val Arg Gly Leu Tyr Leu Pro Phe Glu Ser Gly Thr
265 270 275cca ggc cca acc ggt cgc gtc tac cgc cac gaa atc cca ggc gga cag 979Pro Gly Pro Thr Gly Arg Val Tyr Arg His Glu Ile Pro Gly Gly Gln
280 285 290ttg tcc aac ctg cgt gca cag gcc acc gca ctg ggc ctt gcg gat cgt 1027Leu Ser Asn Leu Arg Ala Gln Ala Thr Ala Leu Gly Leu Ala Asp Arg
295 300 305ttc gaa ctc atc gaa gac aac tac gca agc cgt taatgagatg ctgggacgcc 1080Phe Glu Leu Ile Glu Asp Asn Tyr Ala Ser Arg310 315 320caa 1083<210>116<211>320<212>PRT<213>谷氨酸棒杆菌<400>116Leu Leu Ala Thr Arg Val Arg Ser Phe Ala Leu Lys Pro Ala Ala Glu1 5 10 15Ala Val Ala Lys Leu Thr Pro Glu Leu Leu Ser Val Glu Ala Trp Gly
20 25 30Gly Ala Thr Tyr Asp Val Ala Met Arg Phe Leu Phe Glu Asp Pro Trp
35 40 45Asp Arg Leu Asp Glu Leu Arg Glu Ala Met Pro Asn Val Asn Ile Gln
50 55 60Met Leu Leu Arg Gly Arg Asn Thr Val Gly Tyr Thr Pro Tyr Pro Asp65 70 75 80Ser Val Cys Arg Ala Phe Val Lys Glu Ala Ala Ser Ser Gly Val Asp
85 90 95Ile Phe Arg Ile Phe Asp Ala Leu Asn Asp Val Ser Gln Met Arg Pro
100 105 110Ala Ile Asp Ala Val Leu Glu Thr Asn Thr Ala Val Ala Glu Val Ala
115 120 125Met Ala Tyr Ser Gly Asp Leu Ser Asp Pro Asn Glu Lys Leu Tyr Thr
130 135 140Leu Asp Tyr Tyr Leu Lys Met Ala Glu Glu Ile Val Lys Ser Gly Ala145 150 155 160His Ile Leu Ala Ile Lys Asp Met Ala Gly Leu Leu Arg Pro Ala Ala
165 170 175Val Thr Lys Leu Val Thr Ala Leu Arg Arg Glu Phe Asp Leu Pro Val
180 185 190His Val His Thr His Asp Thr Ala Gly Gly Gln Leu Ala Thr Tyr Phe
195 200 205Ala Ala Ala Gln Ala Gly Ala Asp Ala Val Asp Gly Ala Ser Gly Thr
210 215 220Thr Val Trp His His Leu Pro Ser His Pro Leu Ser Ala Ile Val Ala225 230 235 240Ala Phe Ala His Thr Arg Arg Asp Thr Gly Leu Ser Leu Glu Ala Val
245 250 255Ser Asp Leu Glu Pro Tyr Trp Glu Ala Val Arg Gly Leu Tyr Leu Pro
260 265 270Phe Glu Ser Gly Thr Pro Gly Pro Thr Gly Arg Val Tyr Arg His Glu
275 280 285Ile Pro Gly Gly Gln Leu Ser Asn Leu Arg Ala Gln Ala Thr Ala Leu
290 295 300Gly Leu Ala Asp Arg Phe Glu Leu Ile Glu Asp Asn Tyr Ala Ser Arg305 310 315 320<210>117<211>1083<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1060)<223>FRXA02327<400>117accgctgaag cagcttggcc cagccgcgtt tgctcgtgat ctccgtgagc aggacgcact 60ggcagttact gataccacct tccgcgatgc acaccagtct ttg ctt gcg acc cga 115
Leu Leu Ala Thr Arg
1 5gtc cgc tca ttc gca ctg aag cct gcg gca gag gcc gtc gca aag ctg 163Val Arg Ser Phe Ala Leu Lys Pro Ala Ala Glu Ala Val Ala Lys Leu
10 15 20act cct gag ctt ttg tcc gtg gag gcc tgg ggc ggc gcg acc tac gat 211Thr Pro Glu Leu Leu Ser Val Glu Ala Trp Gly Gly Ala Thr Tyr Asp
25 30 35gtg gcg atg cgt ttc ctc ttt gag gat ccg tgg gac agg ctc gac gag 259Val Ala Met Arg Phe Leu Phe Glu Asp Pro Trp Asp Arg Leu Asp Glu
40 45 50ctg cgc gag gcg atg ccg aat gta aac att cag atg ctg ctt cgc ggc 307Leu Arg Glu Ala Met Pro Asn Val Asn Ile Gln Met Leu Leu Arg Gly
55 60 65cgc aac acc gtg gga tac acc ccg tac cca gac tcc gtc tgc cgc gcg 355Arg Asn Thr Val Gly Tyr Thr Pro Tyr Pro Asp Ser Val Cys Arg Ala70 75 80 85ttt gtt aag gaa gct gcc agc tcc ggc gtg gac atc ttc cgc atc ttc 403Phe Val Lys Glu Ala Ala Ser Ser Gly Val Asp Ile Phe Arg Ile Phe
90 95 100gac gcg ctt aac gac gtc tcc cag atg cgt cca gca atc gac gca gtc 451Asp Ala Leu Asn Asp Val Ser Gln Met Arg Pro Ala Ile Asp Ala Val
105 110 115ctg gag acc aac acc gcg gta gcc gag gtg gct atg gct tat tct ggt 499Leu Glu Thr Asn Thr Ala Val Ala Glu Val Ala Met Ala Tyr Ser Gly
120 125 130gat ctc tct gat cca aat gaa aag ctc tac acc ctg gat tac tac cta 547Asp Leu Ser Asp Pro Asn Glu Lys Leu Tyr Thr Leu Asp Tyr Tyr Leu
135 140 145aag atg gca gag gag atc gtc aag tct ggc gct cac atc ttg gcc att 595Lys Met Ala Glu Glu Ile Val Lys Ser Gly Ala His Ile Leu Ala Ile150 155 160 165aag gat atg gct ggt ctg ctt cgc cca gct gcg gta acc aag ctg gtc 643Lys Asp Met Ala Gly Leu Leu Arg Pro Ala Ala Val Thr Lys Leu Val
170 175 180acc gca ctg cgc cgt gaa ttc gat ctg cca gtg cac gtg cac acc cac 691Thr Ala Leu Arg Arg Glu Phe Asp Leu Pro Val His Val His Thr His
185 190 195gac act gcg ggt ggc cag ctg gca acc tac ttt gct gca gct caa gct 739Asp Thr Ala Gly Gly Gln Leu Ala Thr Tyr Phe Ala Ala Ala Gln Ala
200 205 210ggt gca gat gct gtt gac ggt gct tcc ggc acc act gtc tgg cac cac 787Gly Ala Asp Ala Val Asp Gly Ala Ser Gly Thr Thr Val Trp His His
215 220 225ctc cca agc cat ccc ttg tct gcc att gtt gct gca ttc gcg cac acc 835Leu Pro Ser His Pro Leu Ser Ala Ile Val Ala Ala Phe Ala His Thr230 235 240 245cgt cgc gat acc ggt ttg agc ctc gag gct gtt tct gac ctc gag ccg 883Arg Arg Asp Thr Gly Leu Ser Leu Glu Ala Val Ser Asp Leu Glu Pro
250 255 260tac tgg gaa gca gtg cgc gga ctg tac ctg cca ttt gag tct gga acc 931Tyr Trp Glu Ala Val Arg Gly Leu Tyr Leu Pro Phe Glu Ser Gly Thr
265 270 275cca ggc cca acc ggt cgc gtc tac cgc cac gaa atc cca ggc gga cag 979Pro Gly Pro Thr Gly Arg Val Tyr Arg His Glu Ile Pro Gly Gly Gln
280 285 290ttg tcc aac ctg cgt gca cag gcc acc gca ctg ggc ctt gcg gat cgt 1027Leu Ser Asn Leu Arg Ala Gln Ala Thr Ala Leu Gly Leu Ala Asp Arg
295 300 305ttc gaa ctc atc gaa gac aac tac gca agc cgt taatgagatg ctgggacgcc 1080Phe Glu Leu Ile Glu Asp Asn Tyr Ala Ser Arg310 315 320caa 1083<210>118<211>320<212>PRT<213>谷氨酸棒杆菌<400>118Leu Leu Ala Thr Arg Val Arg Ser Phe Ala Leu Lys Pro Ala Ala Glu1 5 10 15Ala Val Ala Lys Leu Thr Pro Glu Leu Leu Ser Val Glu Ala Trp Gly
20 25 30Gly Ala Thr Tyr Asp Val Ala Met Arg Phe Leu Phe Glu Asp Pro Trp
35 40 45Asp Arg Leu Asp Glu Leu Arg Glu Ala Met Pro Asn Val Asn Ile Gln
50 55 60Met Leu Leu Arg Gly Arg Asn Thr Val Gly Tyr Thr Pro Tyr Pro Asp65 70 75 80Ser Val Cys Arg Ala Phe Val Lys Glu Ala Ala Ser Ser Gly Val Asp
85 90 95Ile Phe Arg Ile Phe Asp Ala Leu Asn Asp Val Ser Gln Met Arg Pro
100 105 110Ala Ile Asp Ala Val Leu Glu Thr Asn Thr Ala Val Ala Glu Val Ala
115 120 125Met Ala Tyr Ser Gly Asp Leu Ser Asp Pro Asn Glu Lys Leu Tyr Thr
130 135 140Leu Asp Tyr Tyr Leu Lys Met Ala Glu Glu Ile Val Lys Ser Gly Ala145 150 155 160His Ile Leu Ala Ile Lys Asp Met Ala Gly Leu Leu Arg Pro Ala Ala
165 170 175Val Thr Lys Leu Val Thr Ala Leu Arg Arg Glu Phe Asp Leu Pro Val
180 185 190His Val His Thr His Asp Thr Ala Gly Gly Gln Leu Ala Thr Tyr Phe
195 200 205Ala Ala Ala Gln Ala Gly Ala Asp Ala Val Asp Gly Ala Ser Gly Thr
210 215 220Thr Val Trp His His Leu Pro Ser His Pro Leu Ser Ala Ile Val Ala225 230 235 240Ala Phe Ala His Thr Arg Arg Asp Thr Gly Leu Ser Leu Glu Ala Val
245 250 255Ser Asp Leu Glu Pro Tyr Trp Glu Ala Val Arg Gly Leu Tyr Leu Pro
260 265 270Phe Glu Ser Gly Thr Pro Gly Pro Thr Gly Arg Val Tyr Arg His Glu
275 280 285Ile Pro Gly Gly Gln Leu Ser Asn Leu Arg Ala Gln Ala Thr Ala Leu
290 295 300Gly Leu Ala Asp Arg Phe Glu Leu Ile Glu Asp Asn Tyr Ala Ser Arg305 310 315 320<210>119<211>1719<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1696)<223>RXN02328<400>119gaagtcgtgc aggtcagggg agtgttgccc gaaaacattg agaggaaaac aaaaaccgat 60gtttgattgg gggaateggg ggttacgata ctaggacgca gtg act gct atc acc 115
Val Thr Ala Ile Thr
1 5ctt ggc ggt ctc ttg ttg aaa gga ata att act cta gtg tcg act cac 163Leu Gly Gly Leu Leu Leu Lys Gly Ile Ile Thr Leu Val Ser Thr His
10 15 20aca tct tca acg ctt cca gca ttc aaa aag atc ttg gta gca aac cgc 211Thr Ser Ser Thr Leu Pro Ala Phe Lys Lys Ile Leu Val Ala Asn Arg
25 30 35ggc gaa atc gcg gtc cgt gct ttc cgt gca gca ctc gaa acc ggt gca 259Gly Glu Ile Ala Val Arg Ala Phe Arg Ala Ala Leu Glu Thr Gly Ala
40 45 50gcc acg gta gct att tac ccc cgt gaa gat cgg gga tca ttc cac cgc 307Ala Thr Val Ala Ile Tyr Pro Arg Glu Asp Arg Gly Ser Phe His Arg
55 60 65tct ttt gct tct gaa gct gtc cgc att ggt acc gaa ggc tca cca gtc 355Ser Phe Ala Ser Glu Ala Val Arg Ile Gly Thr Glu Gly Ser Pro Val70 75 80 85aag gcg tac ctg gac atc gat gaa att atc ggt gca gct aaa aaa gtt 403Lys Ala Tyr Leu Asp Ile Asp Glu Ile Ile Gly Ala Ala Lys Lys Val
90 95 100aaa gca gat gcc att tac ccg gga tac ggc ttc ctg tct gaa aat gcc 451Lys Ala Asp Ala Ile Tyr Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ala
105 110 115cag ctt gcc cgc gag tgt gcg gaa aac ggc att act ttt att ggc cca 499Gln Leu Ala Arg Glu Cys Ala Glu Asn Gly Ile Thr Phe Ile Gly Pro
120 125 130acc cca gag gtt ctt gat ctc acc ggt gat aag tct cgc gcg gta acc 547Thr Pro Glu Val Leu Asp Leu Thr Gly Asp Lys Ser Arg Ala Val Thr
135 140 145gcc gcg aag aag gct ggt ctg cca gtt ttg gcg gaa tcc acc ccg agc 595Ala Ala Lys Lys Ala Gly Leu Pro Val Leu Ala Glu Ser Thr Pro Ser150 155 160 165aaa aac atc gat gag atc gtt aaa agc gct gaa ggc cag act tac ccc 643Lys Asn Ile Asp Glu Ile Val Lys Ser Ala Glu Gly Gln Thr Tyr Pro
170 175 180atc ttt gtg aag gca gtt gcc ggt ggt ggc gga cgc ggt atg cgt ttt 691Ile Phe Val Lys Ala Val Ala Gly Gly Gly Gly Arg Gly Met Arg Phe
185 190 195gtt gct tca cct gat gag ctt cgc aaa tta gca aca gaa gca tct cgt 739Val Ala Ser Pro Asp Glu Leu Arg Lys Leu Ala Thr Glu Ala Ser Arg
200 205 210gaa gct gaa gcg gct ttc ggc gat ggc gcg gta tat gtc gaa cgt gct 787Glu Ala Glu Ala Ala Phe Gly Asp Gly Ala Val Tyr Val Glu Arg Ala
215 220 225gtg att aac cct cag cat att gaa gtg cag atc ctt ggc gat cac act 835Val Ile Asn Pro Gln His Ile Glu Val Gln Ile Leu Gly Asp His Thr230 235 240 245gga gaa gtt gta cac ctt tat gaa cgt gac tgc tca ctg cag cgt cgt 883Gly Glu Val Val His Leu Tyr Glu Arg Asp Cys Ser Leu Gln Arg Arg
250 255 260cac caa aaa gtt gtc gaa att gcg cca gca cag cat ttg gat cca gaa 931His Gln Lys Val Val Glu Ile Ala Pro Ala Gln His Leu Asp Pro Glu
265 270 275ctg cgt gat cgc att tgt gcg gat gca gta aag ttc tgc cgc tcc att 979Leu Arg Asp Arg Ile Cys Ala Asp Ala Val Lys Phe Cys Arg Ser Ile
280 285 290ggt tac cag ggc gcg gga acc gtg gaa ttc ttg gtc gat gaa aag ggc 1027Gly Tyr Gln Gly Ala Gly Thr Val Glu Phe Leu Val Asp Glu Lys Gly
295 300 305aac cac gtc ttc atc gaa atg aac cca cgt atc cag gtt gag cac acc 1075Asn His Val Phe Ile Glu Met Asn Pro Arg Ile Gln Val Glu His Thr310 315 320 325gtg act gaa gaa gtc acc gag gtg gac ctg gtg aag gcg cag atg cgc 1123Val Thr Glu Glu Val Thr Glu Val Asp Leu Val Lys Ala Gln Met Arg
330 335 340ttg gct gct ggt gca acc ttg aag gaa ttg ggt ctg acc caa gat aag 1171Leu Ala Ala Gly Ala Thr Leu Lys Glu Leu Gly Leu Thr Gln Asp Lys
345 350 355atc aag acc cac ggt gca gca ctg cag tgc cgc atc acc acg gaa gat 1219Ile Lys Thr His Gly Ala Ala Leu Gln Cys Arg Ile Thr Thr Glu Asp
360 365 370cca aac aac ggc ttc cgc cca gat acc gga act atc acc gcg tac cgc 1267Pro Asn Asn Gly Phe Arg Pro Asp Thr Gly Thr Ile Thr Ala Tyr Arg
375 380 385tca cca ggc gga gct ggc gtt cgt ctt gac ggt gca gct cag ctc ggt 1315Ser Pro Gly Gly Ala Gly Val Arg Leu Asp Gly Ala Ala Gln Leu Gly390 395 400 405ggc gaa atc acc gca cac ttt gac tcc atg ctg gtg aaa atg acc tgc 1363Gly Glu Ile Thr Ala His Phe Asp Ser Met Leu Val Lys Met Thr Cys
410 415 420cgt ggt tcc gac ttt gaa act gct gtt gct cgt gca cag cgc gcg ttg 1411Arg Gly Ser Asp Phe Glu Thr Ala Val Ala Arg Ala Gln Arg Ala Leu
425 430 435gct gag ttc acc gtg tct ggt gtt gca acc aac att ggt ttc ttg cgt 1459Ala Glu Phe Thr Val Ser Gly Val Ala Thr Asn Ile Gly Phe Leu Arg
440 445 450gcg ttg ctg cgg gaa gag gac ttc act tcc aag cgc atc gcc acc gga 1507Ala Leu Leu Arg Glu Glu Asp Phe Thr Ser Lys Arg Ile Ala Thr Gly
455 460 465ttc att gcc gat cac ccg cac ctc ctt cag gct cca cct gct gat gat 1555Phe Ile Ala Asp His Pro His Leu Leu Gln Ala Pro Pro Ala Asp Asp470 475 480 485gag cag gga cgc atc ctg gat tac ttg gca gat gtc acc gtg aac aag 1603Glu Gln Gly Arg Ile Leu Asp Tyr Leu Ala Asp Val Thr Val Asn Lys
490 495 500cct cat ggt gtg cgt cca aag gat gtt gca gct cct atc gat aag ctg 1651Pro His Gly Val Arg Pro Lys Asp Val Ala Ala Pro Ile Asp Lys Leu
505 510 515cct aac atc aag gat ctg cca ctg cca cgc ggt tcc cgt gac cgc 1696Pro Asn Ile Lys Asp Leu Pro Leu Pro Arg Gly Ser Arg Asp Arg
520 525 530tgaagcagct tggcccagcc gcg 1719<210>120<211>532<212>PRT<213>谷氨酸棒杆菌<400>120Val Thr Ala Ile Thr Leu Gly Gly Leu Leu Leu Lys Gly Ile Ile Thr1 5 10 15Leu Val Ser Thr His Thr Ser Ser Thr Leu Pro Ala Phe Lys Lys Ile
20 25 30Leu Val Ala Asn Arg Gly Glu Ile Ala Val Arg Ala Phe Arg Ala Ala
35 40 45Leu Glu Thr Gly Ala Ala Thr Val Ala Ile Tyr Pro Arg Glu Asp Arg
50 55 60Gly Ser Phe His Arg Ser Phe Ala Ser Glu Ala Val Arg Ile Gly Thr65 70 75 80Glu Gly Ser Pro Val Lys Ala Tyr Leu Asp Ile Asp Glu Ile Ile Gly
85 90 95Ala Ala Lys Lys Val Lys Ala Asp Ala Ile Tyr Pro Gly Tyr Gly Phe
100 105 110Leu Ser Glu Asn Ala Gln Leu Ala Arg Glu Cys Ala Glu Asn Gly Ile
115 120 125Thr Phe Ile Gly Pro Thr Pro Glu Val Leu Asp Leu Thr Gly Asp Lys
130 135 140Ser Arg Ala Val Thr Ala Ala Lys Lys Ala Gly Leu Pro Val Leu Ala145 150 155 160Glu Ser Thr Pro Ser Lys Asn Ile Asp Glu Ile Val Lys Ser Ala Glu
165 170 175Gly Gln Thr Tyr Pro Ile Phe Val Lys Ala Val Ala Gly Gly Gly Gly
180 185 190Arg Gly Met Arg Phe Val Ala Ser Pro Asp Glu Leu Arg Lys Leu Ala
195 200 205Thr Glu Ala Ser Arg Glu Ala Glu Ala Ala Phe Gly Asp Gly Ala Val
210 215 220Tyr Val Glu Arg Ala Val Ile Asn Pro Gln His Ile Glu Val Gln Ile225 230 235 240Leu Gly Asp His Thr Gly Glu Val Val His Leu Tyr Glu Arg Asp Cys
245 250 255Ser Leu Gln Arg Arg His Gln Lys Val Val Glu Ile Ala Pro Ala Gln
260 265 270His Leu Asp Pro Glu Leu Arg Asp Arg Ile Cys Ala Asp Ala Val Lys
275 280 285Phe Cys Arg Ser Ile Gly Tyr Gln Gly Ala Gly Thr Val Glu Phe Leu
290 295 300Val Asp Glu Lys Gly Asn His Val Phe Ile Glu Met Asn Pro Arg Ile305 310 315 320Gln Val Glu His Thr Val Thr Glu Glu Val Thr Glu Val Asp Leu Val
325 330 335Lys Ala Gln Met Arg Leu Ala Ala Gly Ala Thr Leu Lys Glu Leu Gly
340 345 350Leu Thr Gln Asp Lys Ile Lys Thr His Gly Ala Ala Leu Gln Cys Arg
355 360 365Ile Thr Thr Glu Asp Pro Asn Asn Gly Phe Arg Pro Asp Thr Gly Thr
370 375 380Ile Thr Ala Tyr Arg Ser Pro Gly Gly Ala Gly Val Arg Leu Asp Gly385 390 395 400Ala Ala Gln Leu Gly Gly Glu Ile Thr Ala His Phe Asp Ser Met Leu
405 410 415Val Lys Met Thr Cys Arg Gly Ser Asp Phe Glu Thr Ala Val Ala Arg
420 425 430Ala Gln Arg Ala Leu Ala Glu Phe Thr Val Ser Gly Val Ala Thr Asn
435 440 445Ile Gly Phe Leu Arg Ala Leu Leu Arg Glu Glu Asp Phe Thr Ser Lys
450 455 460Arg Ile Ala Thr Gly Phe Ile Ala Asp His Pro His Leu Leu Gln Ala465 470 475 480Val Thr Val Asn Lys Pro His Gly Val Arg Pro Lys Asp Val Ala Ala
500 505 510Pro Ile Asp Lys Leu Pro Asn Ile Lys Asp Leu Pro Leu Pro Arg Gly
515 520 525Ser Arg Asp Arg
530<210>121<211>1406<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1383)<223>FRXA02328<400>121gct tct gaa gct gtc cgc att ggt acc gaa ggc tca cca gtc aag gcg 48Ala Ser Glu Ala Val Arg Ile Gly Thr Glu Gly Ser Pro Val Lys Ala1 5 10 15tac ctg gac atc gat gaa att atc ggt gca gct aaa aaa gtt aaa gca 96Tyr Leu Asp Ile Asp Glu Ile Ile Gly Ala Ala Lys Lys Val Lys Ala
20 25 30gat gcc att tac ccg gga tac ggc ttc ctg tct gaa aat gcc cag ctt 144Asp Ala Ile Tyr Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ala Gln Leu
35 40 45gcc cgc gag tgt gcg gaa aac ggc att act ttt att ggc cca acc cca 192Ala Arg Glu Cys Ala Glu Asn Gly Ile Thr Phe Ile Gly Pro Thr Pro
50 55 60gag gtt ctt gat ctc acc ggt gat aag tct cgc gcg gta acc gcc gcg 240Glu Val Leu Asp Leu Thr Gly Asp Lys Ser Arg Ala Val Thr Ala Ala65 70 75 80aag aag gct ggt ctg cca gtt ttg gcg gaa tcc acc ccg agc aaa aac 288Lys Lys Ala Gly Leu Pro Val Leu Ala Glu Ser Thr Pro Ser Lys Asn
85 90 95atc gat gag atc gtt aaa agc gct gaa ggc cag act tac ccc atc ttt 336Ile Asp Glu Ile Val Lys Ser Ala Glu Gly Gln Thr Tyr Pro Ile Phe
100 105 110gtg aag gca gtt gcc ggt ggt ggc gga cgc ggt atg cgt ttt gtt gct 384Val Lys Ala Val Ala Gly Gly Gly Gly Arg Gly Met Arg Phe Val Ala
115 120 125tca cct gat gag ctt cgc aaa tta gca aca gaa gca tct cgt gaa gct 432Ser Pro Asp Glu Leu Arg Lys Leu Ala Thr Glu Ala Ser Arg Glu Ala
130 135 140gaa gcg gct ttc ggc gat ggc gcg gta tat gtc gaa cgt gct gtg att 480Glu Ala Ala Phe Gly Asp Gly Ala Val Tyr Val Glu Arg Ala Val Ile145 150 155 160aac cct cag cat att gaa gtg cag atc ctt ggc gat cac act gga gaa 528Asn Pro Gln His Ile Glu Val Gln Ile Leu Gly Asp His Thr Gly Glu
165 170 175gtt gta cac ctt tat gaa cgt gac tgc tca ctg cag cgt cgt cac caa 576Val Val His Leu Tyr Glu Arg Asp Cys Ser Leu Gln Arg Arg His Gln
180 185 190aaa gtt gtc gaa att gcg cca gca cag cat ttg gat cca gaa ctg cgt 624Lys Val Val Glu Ile Ala Pro Ala Gln His Leu Asp Pro Glu Leu Arg
195 200 205gat cgc att tgt gcg gat gca gta aag ttc tgc cgc tcc att ggt tac 672Asp Arg Ile Cys Ala Asp Ala Val Lys Phe Cys Arg Ser Ile Gly Tyr
210 215 220cag ggc gcg gga acc gtg gaa ttc ttg gtc gat gaa aag ggc aac cac 720Gln Gly Ala Gly Thr Val Glu Phe Leu Val Asp Glu Lys Gly Asn His225 230 235 240gtc ttc atc gaa atg aac cca cgt atc cag gtt gag cac acc gtg act 768Val Phe Ile Glu Met Asn Pro Arg Ile Gln Val Glu His Thr Val Thr
245 250 255gaa gaa gtc acc gag gtg gac ctg gtg aag gcg cag atg cgc ttg gct 816Glu Glu Val Thr Glu Val Asp Leu Val Lys Ala Gln Met Arg Leu Ala
260 265 270gct ggt gca acc ttg aag gaa ttg ggt ctg acc caa gat aag atc aag 864Ala Gly Ala Thr Leu Lys Glu Leu Gly Leu Thr Gln Asp Lys Ile Lys
275 280 285acc cac ggt gca gca ctg cag tgc cgc atc acc acg gaa gat cca aac 912Thr His Gly Ala Ala Leu Gln Cys Arg Ile Thr Thr Glu Asp Pro Asn
290 295 300aac ggc ttc cgc cca gat acc gga act atc acc gcg tac cgc tca cca 960Asn Gly Phe Arg Pro Asp Thr Gly Thr Ile Thr Ala Tyr Arg Ser Pro305 310 315 320ggc gga gct ggc gtt cgt ctt gac ggt gca gct cag ctc ggt ggc gaa 1008Gly Gly Ala Gly Val Arg Leu Asp Gly Ala Ala Gln Leu Gly Gly Glu
325 330 335atc acc gca cac ttt gac tcc atg ctg gtg aaa atg acc tgc cgt ggt 1056Ile Thr Ala His Phe Asp Ser Met Leu Val Lys Met Thr Cys Arg Gly
340 345 350tcc gac ttt gaa act gct gtt gct cgt gca cag cgc gcg ttg gct gag 1104Ser Asp Phe Glu Thr Ala Val Ala Arg Ala Gln Arg Ala Leu Ala Glu
355 360 365ttc acc gtg tct ggt gtt gca acc aac att ggt ttc ttg cgt gcg ttg 1152Phe Thr Val Ser Gly Val Ala Thr Asn Ile Gly Phe Leu Arg Ala Leu
370 375 380ctg cgg gaa gag gac ttc act tcc aag cgc atc gcc acc gga ttc att 1200Leu Arg Glu Glu Asp Phe Thr Ser Lys Arg Ile Ala Thr Gly Phe Ile385 390 395 400gcc gat cac ccg cac ctc ctt cag gct cca cct gct gat gat gag cag 1248Ala Asp His Pro His Leu Leu Gln Ala Pro Pro Ala Asp Asp Glu Gln
405 410 415gga cgc atc ctg gat tac ttg gca gat gtc acc gtg aac aag cct cat 1296Gly Arg Ile Leu Asp Tyr Leu Ala Asp Val Thr Val Asn Lys Pro His
420 425 430ggt gtg cgt cca aag gat gtt gca gct cct atc gat aag ctg cct aac 1344Gly Val Arg Pro Lys Asp Val Ala Ala Pro Ile Asp Lys Leu Pro Asn
435 440 445atc aag gat ctg cca ctg cca cgc ggt tcc cgt gac cgc tgaagcagct 1393Ile Lys Asp Leu Pro Leu Pro Arg Gly Ser Arg Asp Arg
450 455 460tggcccagcc gcg 1406<210>122<211>461<212>PRT<213>谷氨酸棒杆菌<400>122Ala Ser Glu Ala Val Arg Ile Gly Thr Glu Gly Ser Pro Val Lys Ala1 5 10 15Tyr Leu Asp Ile Asp Glu Ile Ile Gly Ala Ala Lys Lys Val Lys Ala
20 25 30Asp Ala Ile Tyr Pro Gly Tyr Gly Phe Leu Ser Glu Asn Ala Gln Leu
35 40 45Ala Arg Glu Cys Ala Glu Asn Gly Ile Thr Phe Ile Gly Pro Thr Pro
50 55 60Glu Val Leu Asp Leu Thr Gly Asp Lys Ser Arg Ala Val Thr Ala Ala65 70 75 80Lys Lys Ala Gly Leu Pro Val Leu Ala Glu Ser Thr Pro Ser Lys Asn
85 90 95Ile Asp Glu Ile Val Lys Ser Ala Glu Gly Gln Thr Tyr Pro Ile Phe
100 105 110Val Lys Ala Val Ala Gly Gly Gly Gly Arg Gly Met Arg Phe Val Ala
115 120 125Ser Pro Asp Glu Leu Arg Lys Leu Ala Thr Glu Ala Ser Arg Glu Ala
130 135 140Glu Ala Ala Phe Gly Asp Gly Ala Val Tyr Val Glu Arg Ala Val Ile145 150 155 160Asn Pro Gln His Ile Glu Val Gln Ile Leu Gly Asp His Thr Gly Glu
165 170 175Val Val His Leu Tyr Glu Arg Asp Cys Ser Leu Gln Arg Arg His Gln
180 185 190Lys Val Val Glu Ile Ala Pro Ala Gln His Leu Asp Pro Glu Leu Arg
195 200 205Asp Arg Ile Cys Ala Asp Ala Val Lys Phe Cys Arg Ser Ile Gly Tyr
210 215 220Gln Gly Ala Gly Thr Val Glu Phe Leu Val Asp Glu Lys Gly Asn His225 230 235 240Val Phe Ile Glu Met Asn Pro Arg Ile Gln Val Glu His Thr Val Thr
245 250 255Glu Glu Val Thr Glu Val Asp Leu Val Lys Ala Gln Met Arg Leu Ala
260 265 270Ala Gly Ala Thr Leu Lys Glu Leu Gly Leu Thr Gln Asp Lys Ile Lys
275 280 285Thr His Gly Ala Ala Leu Gln Cys Arg Ile Thr Thr Glu Asp Pro Asn
290 295 300Asn Gly Phe Arg Pro Asp Thr Gly Thr Ile Thr Ala Tyr Arg Ser Pro305 310 315 320Gly Gly Ala Gly Val Arg Leu Asp Gly Ala Ala Gln Leu Gly Gly Glu
325 330 335Ile Thr Ala His Phe Asp Ser Met Leu Val Lys Met Thr Cys Arg Gly
340 345 350Ser Asp Phe Glu Thr Ala Val Ala Arg Ala Gln Arg Ala Leu Ala Glu
355 360 365Phe Thr Val Ser Gly Val Ala Thr Asn Ile Gly Phe Leu Arg Ala Leu
370 375 380Leu Arg Glu Glu Asp Phe Thr Ser Lys Arg Ile Ala Thr Gly Phe Ile385 390 395 400Ala Asp His Pro His Leu Leu Gln Ala Pro Pro Ala Asp Asp Glu Gln
405 410 415Gly Arg Ile Leu Asp Tyr Leu Ala Asp Val Thr Val Asn Lys Pro His
420 425 430Gly Val Arg Pro Lys Asp Val Ala Ala Pro Ile Asp Lys Leu Pro Asn
435 440 445Ile Lys Asp Leu Pro Leu Pro Arg Gly Ser Arg Asp Arg
450 455 460<210>123<211>1347<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1324)<223>RXN01048<400>123agctacagat ttagctagtg tttttgttcc agaaccctaa atgaggttct acccttaaca 60gagcttcccg caaaaacacc gattaacaag gctaaatgat atg acc atc gac ctg 115
Met Thr Ile Asp Leu
1 5cag cgt tcc acc caa aac ctc acc cat gag gaa atc ttc gag gca cac 163Gln Arg Ser Thr Gln Asn Leu Thr His Glu Glu Ile Phe Glu Ala His
10 15 20gag ggc gga aag ctc tcc att agt tcc act cgt ccg ctc cgc gac atg 211Glu Gly Gly Lys Leu Ser Ile Ser Ser Thr Arg Pro Leu Arg Asp Met
25 30 35cgc gat ctt tcc ctt gct tac acc cct ggt gtt gct cag gtt tgt gaa 259Arg Asp Leu Ser Leu Ala Tyr Thr Pro Gly Val Ala Gln Val Cys Glu
40 45 50gca atc aag gaa gat cca gag gtt gcg cgc acc cac acg ggc att gga 307Ala Ile Lys Glu Asp Pro Glu Val Ala Arg Thr His Thr Gly Ile Gly
55 60 65aac acc gtc gcg gtt att tcc gac ggc acc gct gtt ctt ggc ctt ggc 355Asn Thr Val Ala Val Ile Ser Asp Gly Thr Ala Val Leu Gly Leu Gly70 75 80 85gat atc gga cct cag gcc tcc ctt ccc gtc atg gag ggc aag gct cag 403Asp Ile Gly Pro Gln Ala Ser Leu Pro Val Met Glu Gly Lys Ala Gln
90 95 100ctg ttt agc tct ttc gct ggc ctg aag gct atc cct atc gtt ttg gac 451Leu Phe Ser Ser Phe Ala Gly Leu Lys Ala Ile Pro Ile Val Leu Asp
105 110 115gtt cac gat gtt gac gct ttg gtt gag acc atc gca gcc atc gcg cct 499Val His Asp Val Asp Ala Leu Val Glu Thr Ile Ala Ala Ile Ala Pro
120 125 130tct ttc ggt gct atc aac ttg gag gac atc tcc gct cct cgt tgc ttc 547Ser Phe Gly Ala Ile Asn Leu Glu Asp Ile Ser Ala Pro Arg Cys Phe
135 140 145gag gtg gag cgc cgc ctc atc gag cgt ctc gat att cca gtt atg cac 595Glu Val Glu Arg Arg Leu Ile Glu Arg Leu Asp Ile Pro Val Met His150 155 160 165gat gac cag cac ggc acc gct gtg gtt atc ctc gct gcg ctg cgc aac 643Asp Asp Gln His Gly Thr Ala Val Val Ile Leu Ala Ala Leu Arg Asn
170 175 180tcc ctg aag ctg ctg gat cgc aag atc gaa gac ctc aag att gtt att 691Ser Leu Lys Leu Leu Asp Arg Lys Ile Glu Asp Leu Lys Ile Val Ile
185 190 195tcc ggc gca ggc gca gcg ggc gtt gca gct gta gat atg ctg acc aac 739Ser Gly Ala Gly Ala Ala Gly Val Ala Ala Val Asp Met Leu Thr Asn
200 205 210gct gga gca acc gac atc gtg gtt ctt gat tcc cga ggc atc atc cac 787Ala Gly Ala Thr Asp Ile Val Val Leu Asp Ser Arg Gly Ile Ile His
215 220 225gac agc cgt gag gat ctt tcc cca gtt aag gct gct ctt gca gag aag 835Asp Ser Arg Glu Asp Leu Ser Pro Val Lys Ala Ala Leu Ala Glu Lys230 235 240 245acc aac cct cgt ggc atc agc ggt ggc atc aat gag gct ttc acc ggc 883Thr Asn Pro Arg Gly Ile Ser Gly Gly Ile Asn Glu Ala Phe Thr Gly
250 255 260gcg gac ctg ttc att ggc gtg tcc ggc ggc aac atc ggc gag gac gct 931Ala Asp Leu Phe Ile Gly Val Ser Gly Gly Asn Ile Gly Glu Asp Ala
265 270 275ctc aaa ctc atg gcc ccg gag cca atc ctg ttc acc ctg gcg aac cca 979Leu Lys Leu Met Ala Pro Glu Pro Ile Leu Phe Thr Leu Ala Asn Pro
280 285 290acc cca gag atc gat cct gag ctg tct cag aag tac ggc gcc atc gtc 1027Thr Pro Glu Ile Asp Pro Glu Leu Ser Gln Lys Tyr Gly Ala Ile Val
295 300 305gcg acc ggg cgc tct gac ctg cct aac cag atc aac aac gtg ctg gcg 1075Ala Thr Gly Arg Ser Asp Leu Pro Asn Gln Ile Asn Asn Val Leu Ala310 315 320 325ttc cca gga att ttc gcc ggc gct ctc gca gcc aag gct aag aag atc 1123Phe Pro Gly Ile Phe Ala Gly Ala Leu Ala Ala Lys Ala Lys Lys Ile
330 335 340acc ccc gag atg aag ctc gcc gct cag agg caa tcg cag aca tcg cag 1171Thr Pro Glu Met Lys Leu Ala Ala Gln Arg Gln Ser Gln Thr Ser Gln
345 350 355ctg agg acc tcg agg tcg gcc gca tcg tgc cta ccg ccc tgg atc ccc 1219Leu Arg Thr Ser Arg Ser Ala Ala Ser Cys Leu Pro Pro Trp Ile Pro
360 365 370gcg ttg ccc caa cag tca agg cag ctg tcc agg ccg tcg cca aag cgc 1267Ala Leu Pro Gln Gln Ser Arg Gln Leu Ser Arg Pro Ser Pro Lys Arg
375 380 385aaa acg ctt aaa aat ttg ctt atc gac gcc tcc ctc ccc gtc gag gcg 1315Lys Thr Leu Lys Asn Leu Leu Ile Asp Ala Ser Leu Pro Val Glu Ala390 395 400 405cca ata ttt taagagcaaa cttgaggccc aca 1347Pro Ile Phe<210>124<211>408<212>PRT<213>谷氨酸棒杆菌<400>124Met Thr Ile Asp Leu Gln Arg Ser Thr Gln Asn Leu Thr His Glu Glu1 5 10 15Ile Phe Glu Ala His Glu Gly Gly Lys Leu Ser Ile Ser Ser Thr Arg
20 25 30Pro Leu Arg Asp Met Arg Asp Leu Ser Leu Ala Tyr Thr Pro Gly Val
35 40 45Ala Gln Val Cys Glu Ala Ile Lys Glu Asp Pro Glu Val Ala Arg Thr
50 55 60His Thr Gly Ile Gly Asn Thr Val Ala Val Ile Ser Asp Gly Thr Ala65 70 75 80Val Leu Gly Leu Gly Asp Ile Gly Pro Gln Ala Ser Leu Pro Val Met
85 90 95Glu Gly Lys Ala Gln Leu Phe Ser Ser Phe Ala Gly Leu Lys Ala Ile
100 105 110Pro Ile Val Leu Asp Val His Asp Val Asp Ala Leu Val Glu Thr Ile
115 120 125Ala Ala Ile Ala Pro Ser Phe Gly Ala Ile Asn Leu Glu Asp Ile Ser
130 135 140Ala Pro Arg Cys Phe Glu Val Glu Arg Arg Leu Ile Glu Arg Leu Asp145 150 155 160Ile Pro Val Met His Asp Asp Gln His Gly Thr Ala Val Val Ile Leu
165 170 175Ala Ala Leu Arg Asn Ser Leu Lys Leu Leu Asp Arg Lys Ile Glu Asp
180 185 190Leu Lys Ile Val Ile Ser Gly Ala Gly Ala Ala Gly Val Ala Ala Val
195 200 205Asp Met Leu Thr Asn Ala Gly Ala Thr Asp Ile Val Val Leu Asp Ser
210 215 220Arg Gly Ile Ile His Asp Ser Arg Glu Asp Leu Ser Pro Val Lys Ala225 230 235 240Ala Leu Ala Glu Lys Thr Asn Pro Arg Gly Ile Ser Gly Gly Ile Asn
245 250 255Glu Ala Phe Thr Gly Ala Asp Leu Phe Ile Gly Val Ser Gly Gly Asn
260 265 270Ile Gly Glu Asp Ala Leu Lys Leu Met Ala Pro Glu Pro Ile Leu Phe
275 280 285Thr Leu Ala Asn Pro Thr Pro Glu Ile Asp Pro Glu Leu Ser Gln Lys
290 295 300Tyr Gly Ala Ile Val Ala Thr Gly Arg Ser Asp Leu Pro Asn Gln Ile305 310 315 320Asn Asn Val Leu Ala Phe Pro Gly Ile Phe Ala Gly Ala Leu Ala Ala
325 330 335Lys Ala Lys Lys Ile Thr Pro Glu Met Lys Leu Ala Ala Gln Arg Gln
340 345 350Ser Gln Thr Ser Gln Leu Arg Thr Ser Arg Ser Ala Ala Ser Cys Leu
355 360 365Pro Pro Trp Ile Pro Ala Leu Pro Gln Gln Ser Arg Gln Leu Ser Arg
370 375 380Pro Ser Pro Lys Arg Lys Thr Leu Lys Asn Leu Leu Ile Asp Ala Ser385 390 395 400Leu Pro Val Glu Ala Pro Ile Phe
405<210>125<211>311<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(288)<223>FRXA01048<400>125cgc tct gac ttg cct aac cag atc aac aac gtg ctg gcg ttc cca gga 48Arg Ser Asp Leu Pro Asn Gln Ile Asn Asn Val Leu Ala Phe Pro Gly1 5 10 15att ttc gcc ggc gct ctc gca gcc aag gct aag aag atc acc ccc gag 96Ile Phe Ala Gly Ala Leu Ala Ala Lys Ala Lys Lys Ile Thr Pro Glu
20 25 30atg aag ctc gcc gct cag agg caa tcg cag aca tcg cag ctg agg acc 144Met Lys Leu Ala Ala Gln Arg Gln Ser Gln Thr Ser Gln Leu Arg Thr
35 40 45tcg agg tcg gcc gca tcg tgc cta ccg ccc tgg atc ccc gcg ttg ccc 192Ser Arg Ser Ala Ala Ser Cys Leu Pro Pro Trp Ile Pro Ala Leu Pro
50 55 60caa cag tca agg cag ctg tcc agg ccg tcg cca aag cgc aaa acg ctt 240Gln Gln Ser Arg Gln Leu Ser Arg Pro Ser Pro Lys Arg Lys Thr Leu65 70 75 80aaa aat ttg ctt atc gac gcc tcc ctc ccc gtc gag gcg cca ata ttt 288Lys Asn Leu Leu Ile Asp Ala Ser Leu Pro Val Glu Ala Pro Ile Phe
85 90 95taagagcaaa cttgaggccc aca 311<210>126<211>96<212>PRT<213>谷氨酸棒杆菌<400>126Arg Ser Asp Leu Pro Asn Gln Ile Asn Asn Val Leu Ala Phe Pro Gly1 5 10 15Ile Phe Ala Gly Ala Leu Ala Ala Lys Ala Lys Lys Ile Thr Pro Glu
20 25 30Met Lys Leu Ala Ala Gln Arg Gln Ser Gln Thr Ser Gln Leu Arg Thr
35 40 45Ser Arg Ser Ala Ala Ser Cys Leu Pro Pro Trp Ile Pro Ala Leu Pro
50 55 60Gln Gln Ser Arg Gln Leu Ser Arg Pro Ser Pro Lys Arg Lys Thr Leu65 70 75 80Lys Asn Leu Leu Ile Asp Ala Ser Leu Pro Val Glu Ala Pro Ile Phe
85 90 95<210>127<211>1063<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1063)<223>FRXA00290<400>127agctacagat ttagctagtg tttttgttcc agaaccctaa atgaggttct acccttaaca 60gagcttcccg caaaaacacc gattaacaag gctaaatgat atg acc atc gac ctg 115
Met Thr Ile Asp Leu
1 5cag cgt tcc acc caa aac ctc acc cat gag gaa atc ttc gag gca cac 163Gln Arg Ser Thr Gln Asn Leu Thr His Glu Glu Ile Phe Glu Ala His
10 15 20gag ggc gga aag ctc tcc att agt tcc act cgt ccg ctc cgc gac atg 211Glu Gly Gly Lys Leu Ser Ile Ser Ser Thr Arg Pro Leu Arg Asp Met
25 30 35cgc gat ctt tcc ctt gct tac acc cct ggt gtt gct cag gtt tgt gaa 259Arg Asp Leu Ser Leu Ala Tyr Thr Pro Gly Val Ala Gln Val Cys Glu
40 45 50gca atc aag gaa gat cca gag gtt gcg cgc acc cac acg ggc att gga 307Ala Ile Lys Glu Asp Pro Glu Val Ala Arg Thr His Thr Gly Ile Gly
55 60 65aac acc gtc gcg gtt att tcc gac ggc acc gct gtt ctt ggc ctt ggc 355Asn Thr Val Ala Val Ile Ser Asp Gly Thr Ala Val Leu Gly Leu Gly70 75 80 85gat atc gga cct cag gcc tcc ctt ccc gtc atg gag ggc aag gct cag 403Asp Ile Gly Pro Gln Ala Ser Leu Pro Val Met Glu Gly Lys Ala Gln
90 95 100ctg ttt agc tct ttc gct ggc ctg aag gct atc cct atc gtt ttg gac 451Leu Phe Ser Ser Phe Ala Gly Leu Lys Ala Ile Pro Ile Val Leu Asp
105 110 115gtt cac gat gtt gac gct ttg gtt gag acc atc gca gcc atc gcg cct 499Val His Asp Val Asp Ala Leu Val Glu Thr Ile Ala Ala Ile Ala Pro
120 125 130tct ttc ggt gct atc aac ttg gag gac atc tcc gct cct cgt tgc ttc 547Ser Phe Gly Ala Ile Asn Leu Glu Asp Ile Ser Ala Pro Arg Cys Phe
135 140 145gag gtg gag cgc cgc ctc atc gag cgt ctc gat att cca gtt atg cac 595Glu Val Glu Arg Arg Leu Ile Glu Arg Leu Asp Ile Pro Val Met His150 155 160 165gat gac cag cac ggc acc gct gtg gtt atc ctc gct gcg ctg cgc aac 643Asp Asp Gln His Gly Thr Ala Val Val Ile Leu Ala Ala Leu Arg Asn
170 175 180tcc ctg aag ctg ctg gat cgc aag atc gaa gac ctc aag att gtt att 691Ser Leu Lys Leu Leu Asp Arg Lys Ile Glu Asp Leu Lys Ile Val Ile
185 190 195tcc ggc gca ggc gca gcg ggc gtt gca gct gta gat atg ctg acc aac 739Ser Gly Ala Gly Ala Ala Gly Val Ala Ala Val Asp Met Leu Thr Asn
200 205 210gct gga gca acc gac atc gtg gtt ctt gat tcc cga ggc atc atc cac 787Ala Gly Ala Thr Asp Ile Val Val Leu Asp Ser Arg Gly Ile Ile His
215 220 225gac agc cgt gag gat ctt tcc cca gtt aag gct gct ctt gca gag aag 835Asp Ser Arg Glu Asp Leu Ser Pro Val Lys Ala Ala Leu Ala Glu Lys230 235 240 245acc aac cct cgt ggc atc agc ggt ggc atc aat gag gct ttc acc ggc 883Thr Asn Pro Arg Gly Ile Ser Gly Gly Ile Asn Glu Ala Phe Thr Gly
250 255 260gcg gac ctg ttc att ggc gtg tcc ggc ggc aac atc ggc gag gac gct 931Ala Asp Leu Phe Ile Gly Val Ser Gly Gly Asn Ile Gly Glu Asp Ala
265 270 275ctc aaa ctc atg gcc ccg gag cca atc ctg ttc acc ctg gcg aac cca 979Leu Lys Leu Met Ala Pro Glu Pro Ile Leu Phe Thr Leu Ala Asn Pro
280 285 290acc cca gag atc gat cct gag ctg tct cag aag tac ggc gcc atc gtc 1027Thr Pro Glu Ile Asp Pro Glu Leu Ser Gln Lys Tyr Gly Ala Ile Val
295 300 305gcg acc ggg ccg gtc ttg acc tgc cta acc aga tca 1063Ala Thr Gly Pro Val Leu Thr Cys Leu Thr Arg Ser310 315 320<210>128<211>321<212>PRT<213>谷氨酸棒杆菌<400>128Met Thr Ile Asp Leu Gln Arg Ser Thr Gln Asn Leu Thr His Glu Glu1 5 10 15Ile Phe Glu Ala His Glu Gly Gly Lys Leu Ser Ile Ser Ser Thr Arg
20 25 30Pro Leu Arg Asp Met Arg Asp Leu Ser Leu Ala Tyr Thr Pro Gly Val
35 40 45Ala Gln Val Cys Glu Ala Ile Lys Glu Asp Pro Glu Val Ala Arg Thr
50 55 60His Thr Gly Ile Gly Asn Thr Val Ala Val Ile Ser Asp Gly Thr Ala65 70 75 80Val Leu Gly Leu Gly Asp Ile Gly Pro Gln Ala Ser Leu Pro Val Met
85 90 95Glu Gly Lys Ala Gln Leu Phe Ser Ser Phe Ala Gly Leu Lys Ala Ile
100 105 110Pro Ile Val Leu Asp Val His Asp Val Asp Ala Leu Val Glu Thr Ile
115 120 125Ala Ala Ile Ala Pro Ser Phe Gly Ala Ile Asn Leu Glu Asp Ile Ser
130 135 140Ala Pro Arg Cys Phe Glu Val Glu Arg Arg Leu Ile Glu Arg Leu Asp145 150 155 160Ile Pro Val Met His Asp Asp Gln His Gly Thr Ala Val Val Ile Leu
165 170 175Ala Ala Leu Arg Asn Ser Leu Lys Leu Leu Asp Arg Lys Ile Glu Asp
180 185 190Leu Lys Ile Val Ile Ser Gly Ala Gly Ala Ala Gly Val Ala Ala Val
195 200 205Asp Met Leu Thr Asn Ala Gly Ala Thr Asp Ile Val Val Leu Asp Ser
210 215 220Arg Gly Ile Ile His Asp Ser Arg Glu Asp Leu Ser Pro Val Lys Ala225 230 235 240Ala Leu Ala Glu Lys Thr Asn Pro Arg Gly Ile Ser Gly Gly Ile Asn
245 250 255Glu Ala Phe Thr Gly Ala Asp Leu Phe Ile Gly Val Ser Gly Gly Asn
260 265 270Ile Gly Glu Asp Ala Leu Lys Leu Met Ala Pro Glu Pro Ile Leu Phe
275 280 285Thr Leu Ala Asn Pro Thr Pro Glu Ile Asp Pro Glu Leu Ser Gln Lys
290 295 300Tyr Gly Ala Ile Val Ala Thr Gly Pro Val Leu Thr Cys Leu Thr Arg305 310 315 320Ser<210>129<211>1065<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1042)<223>RXA02694<400>129attaaaggtg taacaaagga atccgggcac aagctcttgc tgattttctg agctgctttg 60tgggttgtcc ggttagggaa atcaggaagt gggatcgaaa atg aaa gaa acc gtc 115
Met Lys Glu Thr Val
1 5ggt aac aag att gtc ctc att ggc gca gga gat gtt gga gtt gca tac 163Gly Asn Lys Ile Val Leu Ile Gly Ala Gly Asp Val Gly Val Ala Tyr
10 15 20gca tac gca ctg atc aac cag ggc atg gca gat cac ctt gcg atc atc 211Ala Tyr Ala Leu Ile Asn Gln Gly Met Ala Asp His Leu Ala Ile Ile
25 30 35gac atc gat gaa aag aaa ctc gaa ggc aac gtc atg gac tta aac cat 259Asp Ile Asp Glu Lys Lys Leu Glu Gly Asn Val Met Asp Leu Asn His
40 45 50ggt gtt gtg tgg gcc gat tcc cgc acc cgc gtc acc aag ggc acc tac 307Gly Val Val Trp Ala Asp Ser Arg Thr Arg Val Thr Lys Gly Thr Tyr
55 60 65gct gac tgc gaa gac gca gcc atg gtt gtc att tgt gcc ggc gca gcc 355Ala Asp Cys Glu Asp Ala Ala Met Val Val Ile Cys Ala Gly Ala Ala70 75 80 85caa aag cca ggc gag acc cgc ctc cag ctg gtg gac aaa aac gtc aag 403Gln Lys Pro Gly Glu Thr Arg Leu Gln Leu Val Asp Lys Asn Val Lys
90 95 100att atg aaa tcc atc gtc ggc gat gtc atg gac agc gga ttc gac ggc 451Ile Met Lys Ser Ile Val Gly Asp Val Met Asp Ser Gly Phe Asp Gly
105 110 115atc ttc ctc gtg gcg tcc aac cca gtg gat atc ctg acc tac gca gtg 499Ile Phe Leu Val Ala Ser Asn Pro Val Asp Ile Leu Thr Tyr Ala Val
120 125 130tgg aaa ttc tcc ggc ttg gaa tgg aac cgc gtg atc ggc tcc gga act 547Trp Lys Phe Ser Gly Leu Glu Trp Asn Arg Val Ile Gly Ser Gly Thr
135 140 145gtc ctg gac tcc gct cga ttc cgc tac atg ctg ggc gaa ctc tac gaa 595Val Leu Asp Ser Ala Arg Phe Arg Tyr Met Leu Gly Glu Leu Tyr Glu150 155 160 165gtg gca cca agc tcc gtc cac gcc tac atc atc ggc gaa cac ggc gac 643Val Ala Pro Ser Ser Val His Ala Tyr Ile Ile Gly Glu His Gly Asp
170 175 180act gaa ctt cca gtc ctg tcc tcc gcg acc atc gca ggc gta tcg ctt 691Thr Glu Leu Pro Val Leu Ser Ser Ala Thr Ile Ala Gly Val Ser Leu
185 190 195agc cga atg ctg gac aaa gac cca gag ctt gag ggc cgt cta gag aaa 739Ser Arg Met Leu Asp Lys Asp Pro Glu Leu Glu Gly Arg Leu Glu Lys
200 205 210att ttc gaa gac acc cgc gac gct gcc tat cac att atc gac gcc aag 787Ile Phe Glu Asp Thr Arg Asp Ala Ala Tyr His Ile Ile Asp Ala Lys
215 220 225ggc tcc act tcc tac ggc atc ggc atg ggt ctt gct cgc atc acc cgc 835Gly Ser Thr Ser Tyr Gly Ile Gly Met Gly Leu Ala Arg Ile Thr Arg230 235 240 245gca atc cta cag aac caa gac gtt gca gtc cca gtc tct gca ctg ctc 883Ala Ile Leu Gln Asn Gln Asp Val Ala Val Pro Val Ser Ala Leu Leu
250 255 260cac ggt gaa tac ggt gag gaa gac atc tac atc ggc acc cca gct gtg 931His Gly Glu Tyr Gly Glu Glu Asp Ile Tyr Ile Gly Thr Pro Ala Val
265 270 275gtg aac cgc cga ggc atc cgc cgc gtt gtc gaa cta gaa atc acc gac 979Val Asn Arg Arg Gly Ile Arg Arg Val Val Glu Leu Glu Ile Thr Asp
280 285 290cac gag atg gaa cgc ttc aag cat tcc gca aat acc ctg cgc gaa att 1027His Glu Met Glu Arg Phe Lys His Ser Ala Asn Thr Leu Arg Glu Ile
295 300 305cag aag cag ttc ttc taaatctttg gcgcctagtt ggc 1065Gln Lys Gln Phe Phe310<210>130<211>314<212>PRT<213>谷氨酸棒杆菌<400>130Met Lys Glu Thr Val Gly Asn Lys Ile Val Leu Ile Gly Ala Gly Asp1 5 10 15Val Gly Val Ala Tyr Ala Tyr Ala Leu Ile Asn Gln Gly Met Ala Asp
20 25 30His Leu Ala Ile Ile Asp Ile Asp Glu Lys Lys Leu Glu Gly Asn Val
35 40 45Met Asp Leu Asn His Gly Val Val Trp Ala Asp Ser Arg Thr Arg Val
50 55 60Thr Lys Gly Thr Tyr Ala Asp Cys Glu Asp Ala Ala Met Val Val Ile65 70 75 80Cys Ala Gly Ala Ala Gln Lys Pro Gly Glu Thr Arg Leu Gln Leu Val
85 90 95Asp Lys Asn Val Lys Ile Met Lys Ser Ile Val Gly Asp Val Met Asp
100 105 110Ser Gly Phe Asp Gly Ile Phe Leu Val Ala Ser Asn Pro Val Asp Ile
115 120 125Leu Thr Tyr Ala Val Trp Lys Phe Ser Gly Leu Glu Trp Asn Arg Val
130 135 140Ile Gly Ser Gly Thr Val Leu Asp Ser Ala Arg Phe Arg Tyr Met Leu145 150 155 160Gly Glu Leu Tyr Glu Val Ala Pro Ser Ser Val His Ala Tyr Ile Ile
165 170 175Gly Glu His Gly Asp Thr Glu Leu Pro Val Leu Ser Ser Ala Thr Ile
180 185 190Ala Gly Val Ser Leu Ser Arg Met Leu Asp Lys Asp Pro Glu Leu Glu
195 200 205Gly Arg Leu Glu Lys Ile Phe Glu Asp Thr Arg Asp Ala Ala Tyr His
210 215 220Ile Ile Asp Ala Lys Gly Ser Thr Ser Tyr Gly Ile Gly Met Gly Leu225 230 235 240Ala Arg Ile Thr Arg Ala Ile Leu Gln Asn Gln Asp Val Ala Val Pro
245 250 255Val Ser Ala Leu Leu His Gly Glu Tyr Gly Glu Glu Asp Ile Tyr Ile
260 265 270Gly Thr Pro Ala Val Val Asn Arg Arg Gly Ile Arg Arg Val Val Glu
275 280 285Leu Glu Ile Thr Asp His Glu Met Glu Arg Phe Lys His Ser Ala Asn
290 295 300Thr Leu Arg Glu Ile Gln Lys Gln Phe Phe305 310<210>131<211>2967<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2944)<223>RXN00296<400>131tttttgttgg catgtctgac attatcgcac aattcaccac agtaaccggt aacatgttac 60tcaaagcccg cttggatcga atttcacaaa aggacccacc atg act cac acc atc 115
Met Thr His Thr Ile
1 5aaa ttc aac aga ctc gac cca gaa gta ttt agc cag cat tct cgc gcg 163Lys Phe Asn Arg Leu Asp Pro Glu Val Phe Ser Gln His Ser Arg Ala
10 15 20aag ctg cgc acg gat atg aca acc cgt gca gca tat tct tct gat gca 211Lys Leu Arg Thr Asp Met Thr Thr Arg Ala Ala Tyr Ser Ser Asp Ala
25 30 35gga att ttt aga cgc gtc cct gca gct gta gct gaa cca gaa aat gtg 259Gly Ile Phe Arg Arg Val Pro Ala Ala Val Ala Glu Pro Glu Asn Val
40 45 50gaa caa att cgt gat gcc att gct gtt gcg gtg gca cga ggg tgg tct 307Glu Gln Ile Arg Asp Ala Ile Ala Val Ala Val Ala Arg Gly Trp Ser
55 60 65gtt gtt ggg cgc ggt gga gga agc tcg gtt gct gga aat gcg atc ggt 355Val Val Gly Arg Gly Gly Gly Ser Ser Val Ala Gly Asn Ala Ile Gly70 75 80 85gaa ggt ttg atc atc gat acg tca cgc tat ttc aac cgc att tta gat 403Glu Gly Leu Ile Ile Asp Thr Ser Arg Tyr Phe Asn Arg Ile Leu Asp
90 95 100att gat cca gtt gca caa act gca gtt gtg gaa ccc ggt gtg gtg tgt 451Ile Asp Pro Val Ala Gln Thr Ala Val Val Glu Pro Gly Val Val Cys
105 110 115gat gcc ttg cgc gat gca gcc gca gaa ttc gga tta act tac ggc ccg 499Asp Ala Leu Arg Asp Ala Ala Ala Glu Phe Gly Leu Thr Tyr Gly Pro
120 125 130gat cct tcc acg cat tcc cgg tgc acg atc ggt ggc atg gtt gcc aac 547Asp Pro Ser Thr His Ser Arg Cys Thr Ile Gly Gly Met Val Ala Asn
135 140 145aat gcg tgt ggt tca cac tcg gtt gca ttc ggt aca gct gcg gaa aat 595Asn Ala Cys Gly Ser His Ser Val Ala Phe Gly Thr Ala Ala Glu Asn150 155 160 165ctc gtg gat gtc acg ctc atg ctc agc gat ggc cga gaa gtc act gtg 643Leu Val Asp Val Thr Leu Met Leu Ser Asp Gly Arg Glu Val Thr Val
170 175 180aca aaa gat ggc tgc gat gat gct gag atc aat cag aag ctc acc gac 691Thr Lys Asp Gly Cys Asp Asp Ala Glu Ile Asn Gln Lys Leu Thr Asp
185 190 195tta gcg tcc aag aat cag gac ctt att agt aaa gaa ctg ggt cgt ttc 739Leu Ala Ser Lys Asn Gln Asp Leu Ile Ser Lys Glu Leu Gly Arg Phe
200 205 210cct cgc caa gtg tcg ggc tac ggt ttg cat tat ctt gcc cac gac atg 787Pro Arg Gln Val Ser Gly Tyr Gly Leu His Tyr Leu Ala His Asp Met
215 220 225gcc aaa gca atg gcg ggc acc gag gga acc att gga atc att act cgg 835Ala Lys Ala Met Ala Gly Thr Glu Gly Thr Ile Gly Ile Ile Thr Arg230 235 240 245ttg acg gtg aag ttg gtt cca aca ccc aaa gtg aaa gcg ctt gct gtc 883Leu Thr Val Lys Leu Val Pro Thr Pro Lys Val Lys Ala Leu Ala Val
250 255 260ctg gct ttc gac acg gtt ttt gac gcc gcc cga gca gcc gcc aaa ttg 931Leu Ala Phe Asp Thr Val Phe Asp Ala Ala Arg Ala Ala Ala Lys Leu
265 270 275cga ctg cct ggg gta gca acc att gaa ggc atg ggc gga gat ctc ctc 979Arg Leu Pro Gly Val Ala Thr Ile Glu Gly Met Gly Gly Asp Leu Leu
280 285 290gct gcg ctg cgc agt aaa cag gga caa tca gaa gct ggg cag aat ctt 1027Ala Ala Leu Arg Ser Lys Gln Gly Gln Ser Glu Ala Gly Gln Asn Leu
295 300 305cca gga aac cgc atc ggc att gaa gcc ggc gga tgg ttg tac tgc gag 1075Pro Gly Asn Arg Ile Gly Ile Glu Ala Gly Gly Trp Leu Tyr Cys Glu310 315 320 325aca gga agt gac acc ctg cag gcc gcg gta caa gcc gcc gag gaa gtc 1123Thr Gly Ser Asp Thr Leu Gln Ala Ala Val Gln Ala Ala Glu Glu Val
330 335 340gca acc gcc gtt gac acc att gat tac gtg gtc gtg tct gag cct tct 1171Ala Thr Ala Val Asp Thr Ile Asp Tyr Val Val Val Ser Glu Pro Ser
345 350 355gaa atg cgg gaa ttg tgg cgc atc cgt gaa tcc tcg gcg ggc att gtc 1219Glu Met Arg Glu Leu Trp Arg Ile Arg Glu Ser Ser Ala Gly Ile Val
360 365 370acg cgc tta gct gat ggt ggg gaa gcg tgg ccg aat tgg gaa gac tcg 1267Thr Arg Leu Ala Asp Gly Gly Glu Ala Trp Pro Asn Trp Glu Asp Ser
375 380 385gcg gtg cct cca gag aat tta gct gat tat ctc cgc gat ctt tat gcg 1315Ala Val Pro Pro Glu Asn Leu Ala Asp Tyr Leu Arg Asp Leu Tyr Ala390 395 400 405ctg atg gat aag ttc gat tac cag ggt att cca ttt gga cac ttt gga 1363Leu Met Asp Lys Phe Asp Tyr Gln Gly Ile Pro Phe Gly His Phe Gly
410 415 420gaa ggc tgc gtc cac gtt cgc atc agt ttt gat ttc tct acc aag gaa 1411Glu Gly Cys Val His Val Arg Ile Ser Phe Asp Phe Ser Thr Lys Glu
425 430 435ggc ctg aag aaa ttc gag gcg ttc atg aat gaa gcc tcc acc ttg gtg 1459Gly Leu Lys Lys Phe Glu Ala Phe Met Asn Glu Ala Ser Thr Leu Val
440 445 450gcg tct tat ggt ggc agc ctc tcg ggc gag cat gga gac ggt cgc gcc 1507Ala Ser Tyr Gly Gly Ser Leu Ser Gly Glu His Gly Asp Gly Arg Ala
455 460 465cgc tca tcc ttc ctt gac cgc atg tat tca gca gaa atg cgt gca ctc 1555Arg Ser Ser Phe Leu Asp Arg Met Tyr Ser Ala Glu Met Arg Ala Leu470 475 480 485ttc gaa gaa ttc aag ctg att ttc gat ccc cag cgc atc ttc aat ccg 1603Phe Glu Glu Phe Lys Leu Ile Phe Asp Pro Gln Arg Ile Phe Asn Pro
490 495 500gga gtg ttg gtc tgg gca gat cct gtc atg caa gga ctt cgc atg gac 1651Gly Val Leu Val Trp Ala Asp Pro Val Met Gln Gly Leu Arg Met Asp
505 510 515ccg ggc cag cgc gcc ctc gac atc acg ccc gta cac aaa ttc tct aaa 1699Pro Gly Gln Arg Ala Leu Asp Ile Thr Pro Val His Lys Phe Ser Lys
520 525 530gac aaa ggt tcc atg atc aac gcg gtg aat cgc tgc gtg ggt gta tcc 1747Asp Lys Gly Ser Met Ile Asn Ala Val Asn Arg Cys Val Gly Val Ser
535 540 545gca tgc cgc tca gaa tcc gac gcg atg tgc ccg tcc ttc caa atc acc 1795Ala Cys Arg Ser Glu Ser Asp Ala Met Cys Pro Ser Phe Gln Ile Thr550 555 560 565ggc gac gaa gta cat tcc acc aga ggc cgc gcc cgc ttg ctc tct gag 1843Gly Asp Glu Val His Ser Thr Arg Gly Arg Ala Arg Leu Leu Ser Glu
570 575 580atg ttc cgc ggt gaa tcc atc gcc gac ggc tac cgc agc gaa gaa gtc 1891Met Phe Arg Gly Glu Ser Ile Ala Asp Gly Tyr Arg Ser Glu Glu Val
585 590 595aat gaa gcc ctt gac ctg tgc ctt tcc tgc aaa gca tgc gca tcg gaa 1939Asn Glu Ala Leu Asp Leu Cys Leu Ser Cys Lys Ala Cys Ala Ser Glu
600 605 610tgt cca gtc aac gtc gac atg tcc acc tac aaa gcc gaa ttc ctg gac 1987Cys Pro Val Asn Val Asp Met Ser Thr Tyr Lys Ala Glu Phe Leu Asp
615 620 625aaa cac tac gcc ggc cga ctt cgc ccc atg gcc cat tac gtc atg ggc 2035Lys His Tyr Ala Gly Arg Leu Arg Pro Met Ala His Tyr Val Met Gly630 635 640 645tgg ctg ccg ctt ctg gga cac gtt gcc cat aaa ata ccg ctt ctt cct 2083Trp Leu Pro Leu Leu Gly His Val Ala His Lys Ile Pro Leu Leu Pro
650 655 660acg ctt atc gac gcc acc atg cag tca gca ctc acc gcc cca gtg gtg 2131Thr Leu Ile Asp Ala Thr Met Gln Ser Ala Leu Thr Ala Pro Val Val
665 670 675cgc aag gtc ggc ggg ctc gct gat cgc ccg ttg att tcc ttc gcc cac 2179Arg Lys Val Gly Gly Leu Ala Asp Arg Pro Leu Ile Ser Phe Ala His
680 685 690cgc tcg ctg cgc aag tac aag ccg aag aaa aac tca ggt gaa acg gtg 2227Arg Ser Leu Arg Lys Tyr Lys Pro Lys Lys Asn Ser Gly Glu Thr Val
695 700 705gtg ctg tgg ccc gat tcc ttc aac acc aac ctc gac acc gga cca gct 2275Val Leu Trp Pro Asp Ser Phe Asn Thr Asn Leu Asp Thr Gly Pro Ala710 715 720 725cac gca gcg atc aaa act ctt gaa gcc ctc ggt tac aac gtg gtc atc 2323His Ala Ala Ile Lys Thr Leu Glu Ala Leu Gly Tyr Asn Val Val Ile
730 735 740cca gat ggc ttc gtc tgc tgt gga ctc acc tgg cat tcc acc ggc caa 2371Pro Asp Gly Phe Val Cys Cys Gly Leu Thr Trp His Ser Thr Gly Gln
745 750 755ttg agc atg aca aag aaa gtc cta gaa caa acg gcg aaa gtg atg aaa 2419Leu Ser Met Thr Lys Lys Val Leu Glu Gln Thr Ala Lys Val Met Lys
760 765 770ccc tac ctg gac caa ggt cta aca gtc gtt ggt ttg gaa cct tcg tgc 2467Pro Tyr Leu Asp Gln Gly Leu Thr Val Val Gly Leu Glu Pro Ser Cys
775 780 785acc gtc atg ctt caa gat gag gca aca gaa ctc tcc gat aac cct gat 2515Thr Val Met Leu Gln Asp Glu Ala Thr Glu Leu Ser Asp Asn Pro Asp790 795 800 805ctg gca cgc ctt gca gca ctg acc aaa cca ttc gct gag gtc atc gca 2563Leu Ala Arg Leu Ala Ala Leu Thr Lys Pro Phe Ala Glu Val Ile Ala
810 815 820cca aag atc acc gag cta gtc gag tct gga agc ctc cag cta aca gaa 2611Pro Lys Ile Thr Glu Leu Val Glu Ser Gly Ser Leu Gln Leu Thr Glu
825 830 835tca act gcg ctt acc cag gtg cac tgc cac gag cgt tcg cta ggc gac 2659Ser Thr Ala Leu Thr Gln Val His Cys His Glu Arg Ser Leu Gly Asp
840 845 850cca caa caa tcg gca ctc gtt ctt gaa gct ttg ggt gta aaa gat gaa 2707Pro Gln Gln Ser Ala Leu Val Leu Glu Ala Leu Gly Val Lys Asp Glu
855 860 865caa att gcc act ggt tgt tgc ggg ctt gcc gga aac tgg ggc ttt gaa 2755Gln Ile Ala Thr Gly Cys Cys Gly Leu Ala Gly Asn Trp Gly Phe Glu870 875 880 885aaa gac cac gct gaa atg tcc ttc gca ctt ggt gaa cga gag ctg ttc 2803Lys Asp His Ala Glu Met Ser Phe Ala Leu Gly Glu Arg Glu Leu Phe
890 895 900ccc aag gtc aga aaa gca gaa gga cat gtg att gct gac ggt ttc tcc 2851Pro Lys Val Arg Lys Ala Glu Gly His Val Ile Ala Asp Gly Phe Ser
905 910 915tgc cgc acc cag atc gaa caa ggc acc gga aaa caa gca acg cac ctt 2899Cys Arg Thr Gln Ile Glu Gln Gly Thr Gly Lys Gln Ala Thr His Leu
920 925 930gca gag gtg gtc tta agc atc ttg gag caa aac aac atg gca caa 2944Ala Glu Val Val Leu Ser Ile Leu Glu Gln Asn Asn Met Ala Gln
935 940 945taacgatcat gcaacaggtg ctc 2967<210>132<211>948<212>PRT<213>谷氨酸棒杆菌<400>132Met Thr His Thr Ile Lys Phe Asn Arg Leu Asp Pro Glu Val Phe Ser1 5 10 15Gln His Ser Arg Ala Lys Leu Arg Thr Asp Met Thr Thr Arg Ala Ala
20 25 30Tyr Ser Ser Asp Ala Gly Ile Phe Arg Arg Val Pro Ala Ala Val Ala
35 40 45Glu Pro Glu Asn Val Glu Gln Ile Arg Asp Ala Ile Ala Val Ala Val
50 55 60Ala Arg Gly Trp Ser Val Val Gly Arg Gly Gly Gly Ser Ser Val Ala65 70 75 80Gly Asn Ala Ile Gly Glu Gly Leu Ile Ile Asp Thr Ser Arg Tyr Phe
85 90 95Asn Arg Ile Leu Asp Ile Asp Pro Val Ala Gln Thr Ala Val Val Glu
100 105 110Pro Gly Val Val Cys Asp Ala Leu Arg Asp Ala Ala Ala Glu Phe Gly
115 120 125Leu Thr Tyr Gly Pro Asp Pro Ser Thr His Ser Arg Cys Thr Ile Gly
130 135 140Gly Met Val Ala Asn Asn Ala Cys Gly Ser His Sar Val Ala Phe Gly145 150 155 160Thr Ala Ala Glu Asn Leu Val Asp Val Thr Leu Met Leu Ser Asp Gly
165 170 175Arg Glu Val Thr Val Thr Lys Asp Gly Cys Asp Asp Ala Glu Ile Asn
180 185 190Gln Lys Leu Thr Asp Leu Ala Ser Lys Asn Gln Asp Leu Ile Ser Lys
195 200 205Glu Leu Gly Arg Phe Pro Arg Gln Val Ser Gly Tyr Gly Leu His Tyr
210 215 220Leu Ala His Asp Met Ala Lys Ala Met Ala Gly Thr Glu Gly Thr Ile225 230 235 240Gly Ile Ile Thr Arg Leu Thr Val Lys Leu Val Pro Thr Pro Lys Val
245 250 255Lys Ala Leu Ala Val Leu Ala Phe Asp Thr Val Phe Asp Ala Ala Arg
260 265 270Ala Ala Ala Lys Leu Arg Leu Pro Gly Val Ala Thr Ile Glu Gly Met
275 280 285Gly Gly Asp Leu Leu Ala Ala Leu Arg Ser Lys Gln Gly Gln Ser Glu
290 295 300Ala Gly Gln Asn Leu Pro Gly Asn Arg Ile Gly Ile Glu Ala Gly Gly305 310 315 320Trp Leu Tyr Cys Glu Thr Gly Ser Asp Thr Leu Gln Ala Ala Val Gln
325 330 335Ala Ala Glu Glu Val Ala Thr Ala Val Asp Thr Ile Asp Tyr Val Val
340 345 350Val Ser Glu Pro Ser Glu Met Arg Glu Leu Trp Arg Ile Arg Glu Ser
355 360 365Ser Ala Gly Ile Val Thr Arg Leu Ala Asp Gly Gly Glu Ala Trp Pro
370 375 380Asn Trp Glu Asp Ser Ala Val Pro Pro Glu Asn Leu Ala Asp Tyr Leu385 390 395 400Arg Asp Leu Tyr Ala Leu Met Asp Lys Phe Asp Tyr Gln Gly Ile Pro
405 410 415Phe Gly His Phe Gly Glu Gly Cys Val His Val Arg Ile Ser Phe Asp
420 425 430Phe Ser Thr Lys Glu Gly Leu Lys Lys Phe Glu Ala Phe Met Asn Glu
435 440 445Ala Ser Thr Leu Val Ala Ser Tyr Gly Gly Ser Leu Ser Gly Glu His
450 455 460Gly Asp Gly Arg Ala Arg Ser Ser Phe Leu Asp Arg Met Tyr Ser Ala465 470 475 480Glu Met Arg Ala Leu Phe Glu Glu Phe Lys Leu Ile Phe Asp Pro Gln
485 490 495Arg Ile Phe Asn Pro Gly Val Leu Val Trp Ala Asp Pro Val Met Gln
500 505 510Gly Leu Arg Met Asp Pro Gly Gln Arg Ala Leu Asp Ile Thr Pro Val
515 520 525His Lys Phe Ser Lys Asp Lys Gly Ser Met Ile Asn Ala Val Asn Arg
530 535 540Cys Val Gly Val Ser Ala Cys Arg Ser Glu Ser Asp Ala Met Cys Pro545 550 555 560Ser Phe Gln Ile Thr Gly Asp Glu Val His Ser Thr Arg Gly Arg Ala
565 570 575Arg Leu Leu Ser Glu Met Phe Arg Gly Glu Ser Ile Ala Asp Gly Tyr
580 585 590Arg Ser Glu Glu Val Asn Glu Ala Leu Asp Leu Cys Leu Ser Cys Lys
595 600 605Ala Cys Ala Ser Glu Cys Pro Val Asn Val Asp Met Ser Thr Tyr Lys
610 615 620Ala Glu Phe Leu Asp Lys His Tyr Ala Gly Arg Leu Arg Pro Met Ala625 630 635 640His Tyr Val Met Gly Trp Leu Pro Leu Leu Gly His Val Ala His Lys
645 650 655Ile Pro Leu Leu Pro Thr Leu Ile Asp Ala Thr Met Gln Ser Ala Leu
660 665 670Thr Ala Pro Val Val Arg Lys Val Gly Gly Leu Ala Asp Arg Pro Leu
675 680 685Ile Ser Phe Ala His Arg Ser Leu Arg Lys Tyr Lys Pro Lys Lys Asn
690 695 700Ser Gly Glu Thr Val Val Leu Trp Pro Asp Ser Phe Asn Thr Asn Leu705 710 715 720Asp Thr Gly Pro Ala His Ala Ala Ile Lys Thr Leu Glu Ala Leu Gly
725 730 735Tyr Asn Val Val Ile Pro Asp Gly Phe Val Cys Cys Gly Leu Thr Trp
740 745 750His Ser Thr Gly Gln Leu Ser Met Thr Lys Lys Val Leu Glu Gln Thr
755 760 765Ala Lys Val Met Lys Pro Tyr Leu Asp Gln Gly Leu Thr Val Val Gly
770 775 780Leu Glu Pro Ser Cys Thr Val Met Leu Gln Asp Glu Ala Thr Glu Leu785 790 795 800Ser Asp Asn Pro Asp Leu Ala Arg Leu Ala Ala Leu Thr Lys Pro Phe
805 810 815Ala Glu Val Ile Ala Pro Lys Ile Thr Glu Leu Val Glu Ser Gly Ser
820 825 830Leu Gln Leu Thr Glu Ser Thr Ala Leu Thr Gln Val His Cys His Glu
835 840 845Arg Ser Leu Gly Asp Pro Gln Gln Ser Ala Leu Val Leu Glu Ala Leu
850 855 860Gly Val Lys Asp Glu Gln Ile Ala Thr Gly Cys Cys Gly Leu Ala Gly865 870 875 880Asn Trp Gly Phe Glu Lys Asp His Ala Glu Met Ser Phe Ala Leu Gly
885 890 895Glu Arg Glu Leu Phe Pro Lys Val Arg Lys Ala Glu Gly His Val Ile
900 905 910Ala Asp Gly Phe Ser Cys Arg Thr Gln Ile Glu Gln Gly Thr Gly Lys
915 920 925Gln Ala Thr His Leu Ala Glu Val Val Leu Ser Ile Leu Glu Gln Asn
930 935 940Asn Met Ala Gln945<210>133<211>2858<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2835)<223>FRXA00296<400>133acc atc aaa ttc aac aga ctc gac cca gaa gta ttt agc cag cat tct 48Thr Ile Lys Phe Asn Arg Leu Asp Pro Glu Val Phe Ser Gln His Ser1 5 10 15cgc gcg aag ctg cgc acg gat atg aca acc cgt gca gca tat tct tct 96Arg Ala Lys Leu Arg Thr Asp Met Thr Thr Arg Ala Ala Tyr Ser Ser
20 25 30gat gca gga att ttt aga cgc gtc cct gca gct gta gct gaa cca gaa 144Asp Ala Gly Ile Phe Arg Arg Val Pro Ala Ala Val Ala Glu Pro Glu
35 40 45aat gtg gaa caa att cgt gat gcc att gct gtt gcg gtg gca cga ggg 192Asn Val Glu Gln Ile Arg Asp Ala Ile Ala Val Ala Val Ala Arg Gly
50 55 60tgg tct gtt gtt ggg cgc ggt gga gga agc tcg gtt gct gga aat gcg 240Trp Ser Val Val Gly Arg Gly Gly Gly Ser Ser Val Ala Gly Asn Ala65 70 75 80atc ggt gaa ggt ttg atc atc gat acg tca cgc tat ttc aac cgc att 288Ile Gly Glu Gly Leu Ile Ile Asp Thr Ser Arg Tyr Phe Asn Arg Ile
85 90 95tta gat att gat cca gtt gca caa act gca gtt gtg gaa ccc ggt gtg 336Leu Asp Ile Asp Pro Val Ala Gln Thr Ala Val Val Glu Pro Gly Val
100 105 110gtg tgt gat gcc ttg cgc gat gca gcc gca gaa ttc gga tta act tac 384Val Cys Asp Ala Leu Arg Asp Ala Ala Ala Glu Phe Gly Leu Thr Tyr
115 120 125ggc ccg gat cct tcc acg cat tcc cgg tgc acg atc ggt ggc atg gtt 432Gly Pro Asp Pro Ser Thr His Ser Arg Cys Thr Ile Gly Gly Met Val
130 135 140gcc aac aat gcg tgt ggt tca cac tcg gtt gca ttc ggt aca gct gcg 480Ala Asn Asn Ala Cys Gly Ser His Ser Val Ala Phe Gly Thr Ala Ala145 150 155 160gaa aat ctc gtg gat gtc acg ctc atg ctc agc gat ggc cga gaa gtc 528Glu Asn Leu Val Asp Val Thr Leu Met Leu Ser Asp Gly Arg Glu Val
165 170 175act gtg aca aaa gat ggc tgc gat gat gct gag atc aat cag aag ctc 576Thr Val Thr Lys Asp Gly Cys Asp Asp Ala Glu Ile Asn Gln Lys Leu
180 185 190acc gac tta gcg tcc aag aat cag gac ctt att agt aaa gaa ctg ggt 624Thr Asp Leu Ala Ser Lys Asn Gln Asp Leu Ile Ser Lys Glu Leu Gly
195 200 205cgt ttc cct cgc caa gtg tcg ggc tac ggt ttg cat tat ctt gcc cac 672Arg Phe Pro Arg Gln Val Ser Gly Tyr Gly Leu His Tyr Leu Ala His
210 215 220gac atg gcc aaa gca atg gcg ggc acc gag gga acc att gga atc att 720Asp Met Ala Lys Ala Met Ala Gly Thr Glu Gly Thr Ile Gly Ile Ile225 230 235 240act cgg ttg acg gtg aag ttg gtt cca aca ccc aaa gtg aaa gcg ctt 768Thr Arg Leu Thr Val Lys Leu Val Pro Thr Pro Lys Val Lys Ala Leu
245 250 255gct gtc ctg gct ttc gac acg gtt ttt gac gcc gcc cga gca gcc gcc 816Ala Val Leu Ala Phe Asp Thr Val Phe Asp Ala Ala Arg Ala Ala Ala
260 265 270aaa ttg cga ctg cct ggg gta gca acc att gaa ggc atg ggc gga gat 864Lys Leu Arg Leu Pro Gly Val Ala Thr Ile Glu Gly Met Gly Gly Asp
275 280 285ctc ctc gct gcg ctg cgc agt aaa cag gga caa tca gaa gct ggg cag 912Leu Leu Ala Ala Leu Arg Ser Lys Gln Gly Gln Ser Glu Ala Gly Gln
290 295 300aat ctt cca gga aac cgc atc ggc att gaa gcc ggc gga tgg ttg tac 960Asn Leu Pro Gly Asn Arg Ile Gly Ile Glu Ala Gly Gly Trp Leu Tyr305 310 315 320tgc gag aca gga agt gac acc ctg cag gcc gcg gta caa gcc gcc gag 1008Cys Glu Thr Gly Ser Asp Thr Leu Gln Ala Ala Val Gln Ala Ala Glu
325 330 335gaa gtc gca acc gcc gtt gac acc att gat tac gtg gtc gtg tct gag 1056Glu Val Ala Thr Ala Val Asp Thr Ile Asp Tyr Val Val Val Ser Glu
340 345 350cct tct gaa atg cgg gaa ttg tgg cgc atc cgt gaa tcc tcg gcg ggc 1104Pro Ser Glu Met Arg Glu Leu Trp Arg Ile Arg Glu Ser Ser Ala Gly
355 360 365att gtc acg cgc tta gct gat ggt ggg gaa gcg tgg ccg aat tgg gaa 1152Ile Val Thr Arg Leu Ala Asp Gly Gly Glu Ala Trp Pro Asn Trp Glu
370 375 380gac tcg gcg gtg cct cca gag aat tta gct gat tat ctc cgc gat ctt 1200Asp Ser Ala Val Pro Pro Glu Asn Leu Ala Asp Tyr Leu Arg Asp Leu385 390 395 400tat gcg ctg atg gat aag ttc gat tac cag ggt att cca ttt gga cac 1248Tyr Ala Leu Met Asp Lys Phe Asp Tyr Gln Gly Ile Pro Phe Gly His
405 410 415ttt gga gaa ggc tgc gtc cac gtt cgc atc agt ttt gat ttc tct acc 1296Phe Gly Glu Gly Cys Val His Val Arg Ile Ser Phe Asp Phe Ser Thr
420 425 430aag gaa ggc ctg aag aaa ttc gag gcg ttc atg aat gaa gcc tcc acc 1344Lys Glu Gly Leu Lys Lys Phe Glu Ala Phe Met Asn Glu Ala Ser Thr
435 440 445ttg gtg gcg tct tat ggt ggc agc ctc tcg ggc gag cat gga gac ggt 1392Leu Val Ala Ser Tyr Gly Gly Ser Leu Ser Gly Glu His Gly Asp Gly
450 455 460cgc gcc cgc tca tcc ttc ctt gac cgc atg tat tca gca gaa atg cgt 1440Arg Ala Arg Ser Ser Phe Leu Asp Arg Met Tyr Ser Ala Glu Met Arg465 470 475 480gca ctc ttc gaa gaa ttc aag ctg att ttc gat ccc cag cgc atc ttc 1488Ala Leu Phe Glu Glu Phe Lys Leu Ile Phe Asp Pro Gln Arg Ile Phe
485 490 495aat ccg gga gtg ttg gtc tgg gca gat cct gtc atg caa gga ctt cgc 1536Asn Pro Gly Val Leu Val Trp Ala Asp Pro Val Met Gln Gly Leu Arg
500 505 510atg gac ccg ggc cag cgc gcc ctc gac atc acg ccc gta cac aaa ttc 1584Met Asp Pro Gly Gln Arg Ala Leu Asp Ile Thr Pro Val His Lys Phe
515 520 525tct aaa gac aaa ggt tcc atg atc aac gcg gtg aat cgc tgc gtg ggt 1632Ser Lys Asp Lys Gly Ser Met Ile Asn Ala Val Asn Arg Cys Val Gly
530 535 540gta tcc gca tgc cgc tca gaa tcc gac gcg atg tgc ccg tcc ttc caa 1680Val Ser Ala Cys Arg Ser Glu Ser Asp Ala Met Cys Pro Ser Phe Gln545 550 555 560atc acc ggc gac gaa gta cat tcc acc aga ggc cgc gcc cgc ttg ctc 1728Ile Thr Gly Asp Glu Val His Ser Thr Arg Gly Arg Ala Arg Leu Leu
565 570 575tct gag atg ttc cgc ggt gaa tcc atc gcc gac ggc tac cgc agc gaa 1776Ser Glu Met Phe Arg Gly Glu Ser Ile Ala Asp Gly Tyr Arg Ser Glu
580 585 590gaa gtc aat gaa gcc ctt gac ctg tgc ctt tcc tgc aaa gca tgc gca 1824Glu Val Asn Glu Ala Leu Asp Leu Cys Leu Ser Cys Lys Ala Cys Ala
595 600 605tcg gaa tgt cca gtc aac gtc gac atg tcc acc tac aaa gcc gaa ttc 1872Ser Glu Cys Pro Val Asn Val Asp Met Ser Thr Tyr Lys Ala Glu Phe
610 615 620ctg gac aaa cac tac gcc ggc cga ctt cgc ccc atg gcc cat tac gtc 1920Leu Asp Lys His Tyr Ala Gly Arg Leu Arg Pro Met Ala His Tyr Val625 630 635 640atg ggc tgg ctg ccg ctt ctg gga cac gtt gcc cat aaa ata ccg ctt 1968Met Gly Trp Leu Pro Leu Leu Gly His Val Ala His Lys Ile Pro Leu
645 650 655ctt cct acg ctt atc gac gcc acc atg Gag tca gca ctc acc gcc cca 2016Leu Pro Thr Leu Ile Asp Ala Thr Met Gln Ser Ala Leu Thr Ala Pro
660 665 670gtg gtg cgc aag gtc ggc ggg ctc gct gat cgc ccg ttg att tcc ttc 2064Val Val Arg Lys Val Gly Gly Leu Ala Asp Arg Pro Leu Ile Ser Phe
675 680 685gcc cac cgc tcg ctg cgc aag tac aag ccg aag aaa aac tca ggt gaa 2112Ala His Arg Ser Leu Arg Lys Tyr Lys Pro Lys Lys Asn Ser Gly Glu
690 695 700acg gtg gtg ctg tgg ccc gat tcc ttc aac acc aac ctc gac acc gga 2160Thr Val Val Leu Trp Pro Asp Ser Phe Asn Thr Asn Leu Asp Thr Gly705 710 715 720cca gct cac gca gcg atc aaa act ctt gaa gcc ctc ggt tac aac gtg 2208Pro Ala His Ala Ala Ile Lys Thr Leu Glu Ala Leu Gly Tyr Asn Val
725 730 735gtc atc cca gat ggc ttc gtc tgc tgt gga ctc acc tgg cat tcc acc 2256Val Ile Pro Asp Gly Phe Val Cys Cys Gly Leu Thr Trp His Ser Thr
740 745 750ggc caa ttg agc atg aca aag aaa gtc cta gaa caa acg gcg aaa gtg 2304Gly Gln Leu Ser Met Thr Lys Lys Val Leu Glu Gln Thr Ala Lys Val
755 760 765atg aaa ccc tac ctg gac caa ggt cta aca gtc gtt ggt ttg gaa cct 2352Met Lys Pro Tyr Leu Asp Gln Gly Leu Thr Val Val Gly Leu Glu Pro
770 775 780tcg tgc acc gtc atg ctt caa gat gag gca aca gaa ctc tcc gat aac 2400Ser Cys Thr Val Met Leu Gln Asp Glu Ala Thr Glu Leu Ser Asp Asn785 790 795 800cct gat ctg gca cgc ctt gca gca ctg acc aaa cca ttc gct gag gtc 2448Pro Asp Leu Ala Arg Leu Ala Ala Leu Thr Lys Pro Phe Ala Glu Val
805 810 815atc gca cca aag atc acc gag cta gtc gag tct gga agc ctc cag cta 2496Ile Ala Pro Lys Ile Thr Glu Leu Val Glu Ser Gly Ser Leu Gln Leu
820 825 830aca gaa tca act gcg ctt acc cag gtg cac tgc cac gag cgt tcg cta 2544Thr Glu Ser Thr Ala Leu Thr Gln Val His Cys His Glu Arg Ser Leu
835 840 845ggc gac cca caa caa tcg gca ctc gtt ctt gaa gct ttg ggt gta aaa 2592Gly Asp Pro Gln Gln Ser Ala Leu Val Leu Glu Ala Leu Gly Val Lys
850 855 860gat gaa caa att gcc act ggt tgt tgc ggg ctt gcc gga aac tgg ggc 2640Asp Glu Gln Ile Ala Thr Gly Cys Cys Gly Leu Ala Gly Asn Trp Gly865 870 875 880ttt gaa aaa gac cac gct gaa atg tcc ttc gca ctt ggt gaa cga gag 2688Phe Glu Lys Asp His Ala Glu Met Ser Phe Ala Leu Gly Glu Arg Glu
885 890 895ctg ttc ccc aag gtc aga aaa gca gaa gga cat gtg att gct gac ggt 2736Leu Phe Pro Lys Val Arg Lys Ala Glu Gly His Val Ile Ala Asp Gly
900 905 910ttc tcc tgc cgc acc cag atc gaa caa ggc acc gga aaa caa gca acg 2784Phe Ser Cys Arg Thr Gln Ile Glu Gln Gly Thr Gly Lys Gln Ala Thr
915 920 925cac ctt gca gag gtg gtc tta agc atc ttg gag caa aac aac atg gca 2832His Leu Ala Glu Val Val Leu Ser Ile Leu Glu Gln Asn Asn Met Ala
930 935 940caa taacgatcat gcaacaggtg ctc 2858Gln945<210>134<211>945<212>PRT<213>谷氨酸棒杆菌<400>134Thr Ile Lys Phe Asn Arg Leu Asp Pro Glu Val Phe Ser Gln His Ser1 5 10 15Arg Ala Lys Leu Arg Thr Asp Met Thr Thr Arg Ala Ala Tyr Ser Ser
20 25 30Asp Ala Gly Ile Phe Arg Arg Val Pro Ala Ala Val Ala Glu Pro Glu
35 40 45Asn Val Glu Gln Ile Arg Asp Ala Ile Ala Val Ala Val Ala Arg Gly
50 55 60Trp Ser Val Val Gly Arg Gly Gly Gly Ser Ser Val Ala Gly Asn Ala65 70 75 80Ile Gly Glu Gly Leu Ile Ile Asp Thr Ser Arg Tyr Phe Asn Arg Ile
85 90 95Leu Asp Ile Asp Pro Val Ala Gln Thr Ala Val Val Glu Pro Gly Val
100 105 110Val Cys Asp Ala Leu Arg Asp Ala Ala Ala Glu Phe Gly Leu Thr Tyr
115 120 125Gly Pro Asp Pro Ser Thr His Ser Arg Cys Thr Ile Gly Gly Met Val
130 135 140Ala Asn Asn Ala Cys Gly Ser His Ser Val Ala Phe Gly Thr Ala Ala145 150 155 160Glu Asn Leu Val Asp Val Thr Leu Met Leu Ser Asp Gly Arg Glu Val
165 170 175Thr Val Thr Lys Asp Gly Cys Asp Asp Ala Glu Ile Asn Gln Lys Leu
180 185 190Thr Asp Leu Ala Ser Lys Asn Gln Asp Leu Ile Ser Lys Glu Leu Gly
195 200 205Arg Phe Pro Arg Gln Val Ser Gly Tyr Gly Leu His Tyr Leu Ala His
210 215 220Asp Met Ala Lys Ala Met Ala Gly Thr Glu Gly Thr Ile Gly Ile Ile225 230 235 240Thr Arg Leu Thr Val Lys Leu Val Pro Thr Pro Lys Val Lys Ala Leu
245 250 255Ala Val Leu Ala Phe Asp Thr Val Phe Asp Ala Ala Arg Ala Ala Ala
260 265 270Lys Leu Arg Leu Pro Gly Val Ala Thr Ile Glu Gly Met Gly Gly Asp
275 280 285Leu Leu Ala Ala Leu Arg Ser Lys Gln Gly Gln Ser Glu Ala Gly Gln
290 295 300Asn Leu Pro Gly Asn Arg Ile Gly Ile Glu Ala Gly Gly Trp Leu Tyr305 310 315 320Cys Glu Thr Gly Ser Asp Thr Leu Gln Ala Ala Val Gln Ala Ala Glu
325 330 335Glu Val Ala Thr Ala Val Asp Thr Ile Asp Tyr Val Val Val Ser Glu
340 345 350Pro Ser Glu Met Arg Glu Leu Trp Arg Ile Arg Glu Ser Ser Ala Gly
355 360 365Ile Val Thr Arg Leu Ala Asp Gly Gly Glu Ala Trp Pro Asn Trp Glu
370 375 380Asp Ser Ala Val Pro Pro Glu Asn Leu Ala Asp Tyr Leu Arg Asp Leu385 390 395 400Tyr Ala Leu Met Asp Lys Phe Asp Tyr Gln Gly Ile Pro Phe Gly His
405 410 415Phe Gly Glu Gly Cys Val His Val Arg Ile Ser Phe Asp Phe Ser Thr
420 425 430Lys Glu Gly Leu Lys Lys Phe Glu Ala Phe Met Asn Glu Ala Ser Thr
435 440 445Leu Val Ala Ser Tyr Gly Gly Ser Leu Ser Gly Glu His Gly Asp Gly
450 455 460Arg Ala Arg Ser Ser Phe Leu Asp Arg Met Tyr Ser Ala Glu Met Arg465 470 475 480Ala Leu Phe Glu Glu Phe Lys Leu Ile Phe Asp Pro Gln Arg Ile Phe
485 490 495Asn Pro Gly Val Leu Val Trp Ala Asp Pro Val Met Gln Gly Leu Arg
500 505 510Met Asp Pro Gly Gln Arg Ala Leu Asp Ile Thr Pro Val His Lys Phe
515 520 525Ser Lys Asp Lys Gly Ser Met Ile Asn Ala Val Asn Arg Cys Val Gly
530 535 540Val Ser Ala Cys Arg Ser Glu Ser Asp Ala Met Cys Pro Ser Phe Gln545 550 555 560Ile Thr Gly Asp Glu Val His Ser Thr Arg Gly Arg Ala Arg Leu Leu
565 570 575Ser Glu Met Phe Arg Gly Glu Ser Ile Ala Asp Gly Tyr Arg Ser Glu
580 585 590Glu Val Asn Glu Ala Leu Asp Leu Cys Leu Ser Cys Lys Ala Cys Ala
595 600 605Ser Glu Cys Pro Val Asn Val Asp Met Ser Thr Tyr Lys Ala Glu Phe
610 615 620Leu Asp Lys His Tyr Ala Gly Arg Leu Arg Pro Met Ala His Tyr Val625 630 635 640Met Gly Trp Leu Pro Leu Leu Gly His Val Ala His Lys Ile Pro Leu
645 650 655Leu Pro Thr Leu Ile Asp Ala Thr Met Gln Ser Ala Leu Thr Ala Pro
660 665 670Val Val Arg Lys Val Gly Gly Leu Ala Asp Arg Pro Leu Ile Ser Phe
675 680 685Ala His Arg Ser Leu Arg Lys Tyr Lys Pro Lys Lys Asn Ser Gly Glu
690 695 700Thr Val Val Leu Trp Pro Asp Ser Phe Asn Thr Asn Leu Asp Thr Gly705 710 715 720Pro Ala His Ala Ala Ile Lys Thr Leu Glu Ala Leu Gly Tyr Asn Val
725 730 735Val Ile Pro Asp Gly Phe Val Cys Cys Gly Leu Thr Trp His Ser Thr
740 745 750Gly Gln Leu Ser Met Thr Lys Lys Val Leu Glu Gln Thr Ala Lys Val
755 760 765Met Lys Pro Tyr Leu Asp Gln Gly Leu Thr Val Val Gly Leu Glu Pro
770 775 780Ser Cys Thr Val Met Leu Gln Asp Glu Ala Thr Glu Leu Ser Asp Asn785 790 795 800Pro Asp Leu Ala Arg Leu Ala Ala Leu Thr Lys Pro Phe Ala Glu Val
805 810 815Ile Ala Pro Lys Ile Thr Glu Leu Val Glu Ser Gly Ser Leu Gln Leu
820 825 830Thr Glu Ser Thr Ala Leu Thr Gln Val His Cys His Glu Arg Ser Leu
835 840 845Gly Asp Pro Gln Gln Ser Ala Leu Val Leu Glu Ala Leu Gly Val Lys
850 855 860Asp Glu Gln Ile Ala Thr Gly Cys Cys Gly Leu Ala Gly Asn Trp Gly865 870 875 880Phe Glu Lys Asp His Ala Glu Met Sar Phe Ala Leu Gly Glu Arg Glu
885 890 895Leu Phe Pro Lys Val Arg Lys Ala Glu Gly His Val Ile Ala Asp Gly
900 905 910Phe Ser Cys Arg Thr Gln Ile Glu Gln Gly Thr Gly Lys Gln Ala Thr
915 920 925His Leu Ala Glu Val Val Leu Ser Ile Leu Glu Gln Asn Asn Met Ala
930 935 940Gln945<210>135<211>1383<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1360)<223>RXA01901<400>135gcatgttgcc ttctctctgt gatcgcctcg ttcttcatcc aacgcgtcgc gcaccaagag 60aactaaaatc taagtaaaac ccctccgaaa ggaaccaccc atg gtg aaa cgt caa 115
Met Val Lys Arg Gln
1 5ctg ccc aac ccc gca gaa cta ctc gaa ctc atg aag ttc aaa aag cca 163Leu Pro Asn Pro Ala Glu Leu Leu Glu Leu Met Lys Phe Lys Lys Pro
10 15 20gag ctc aac ggc aag aaa cga cgc cta gac tcc gcg ctc acc atc tac 211Glu Leu Asn Gly Lys Lys Arg Arg Leu Asp Ser Ala Leu Thr Ile Tyr
25 30 35gac ctg cgt aaa att gct aaa cga cgc acc cca gct gcc gcg ttc gac 259Asp Leu Arg Lys Ile Ala Lys Arg Arg Thr Pro Ala Ala Ala Phe Asp
40 45 50tac acc gac ggc gca gcc gag gcc gaa ctc tca atc aca cgc gca cgt 307Tyr Thr Asp Gly Ala Ala Glu Ala Glu Leu Ser Ile Thr Arg Ala Arg
55 60 65gaa gca ttc gaa aac atc gaa ttc cac cca gac atc ctc aag cct gca 355Glu Ala Phe Glu Asn Ile Glu Phe His Pro Asp Ile Leu Lys Pro Ala70 75 80 85gaa cac gta gac acc acc acc caa atc ctg ggc gga acc tcc tcc atg 403Glu His Val Asp Thr Thr Thr Gln Ile Leu Gly Gly Thr Ser Ser Met
90 95 100cca ttc ggc atc gca cca acc ggc ttc acc cgc ctc atg cag acc gaa 451Pro Phe Gly Ile Ala Pro Thr Gly Phe Thr Arg Leu Met Gln Thr Glu
105 110 115ggt gaa atc gca ggt gcc gga gct gca ggc gct gca gga att cct ttc 499Gly Glu Ile Ala Gly Ala Gly Ala Ala Gly Ala Ala Gly Ile Pro Phe
120 125 130acc ctg tcc acc ctg ggc act acc tcc atc gaa gac gtc aag gcc acc 547Thr Leu Ser Thr Leu Gly Thr Thr Ser Ile Glu Asp Val Lys Ala Thr
135 140 145aac ccc aac ggc cga aac tgg ttc cag ctc tac gtc atg cgc gac cgc 595Asn Pro Asn Gly Arg Asn Trp Phe Gln Leu Tyr Val Met Arg Asp Arg150 155 160 165gaa atc tcc tac ggc ctc gtc gaa cgc gca gcc aaa gca gga ttc gac 643Glu Ile Ser Tyr Gly Leu Val Glu Arg Ala Ala Lys Ala Gly Phe Asp
170 175 180acc ctg atg ttc acc gtg gat acc ccc atc gcc ggc tac cgc atc cgc 691Thr Leu Met Phe Thr Val Asp Thr Pro Ile Ala Gly Tyr Arg Ile Arg
185 190 195gat tcc cgc aac gga ttc tcc atc ccg cca cag ctg acc cca tcc acc 739Asp Ser Arg Asn Gly Phe Ser Ile Pro Pro Gln Leu Thr Pro Ser Thr
200 205 210gtg ctc aat gca atc cca cgc cca tgg tgg tgg atc gac ttc ctg acc 787Val Leu Asn Ala Ile Pro Arg Pro Trp Trp Trp Ile Asp Phe Leu Thr
215 220 225acc cca acc ctt gag ttc gca tcc ctt tcc tcg acc ggc gga acc gtg 835Thr Pro Thr Leu Glu Phe Ala Ser Leu Ser Ser Thr Gly Gly Thr Val230 235 240 245ggc gac ctc ctc aac tcc gcg atg gat ccc acc att tct tac gaa gac 883Gly Asp Leu Leu Asn Ser Ala Met Asp Pro Thr Ile Ser Tyr Glu Asp
250 255 260ctc aag gtc atc cgt gaa atg tgg cca ggc aag ctc gta gtc aag ggt 931Leu Lys Val Ile Arg Glu Met Trp Pro Gly Lys Leu Val Val Lys Gly
265 270 275gtc cag aac gtt gaa gac tcc gtc aaa ctc ctc gac caa ggc gtc gac 979Val Gln Asn Val Glu Asp Ser Val Lys Leu Leu Asp Gln Gly Val Asp
280 285 290ggc ctc atc ctc tcc aac cac ggt ggc cgt caa ctc gac cgc gca cca 1027Gly Leu Ile Leu Ser Asn His Gly Gly Arg Gln Leu Asp Arg Ala Pro
295 300 305gtc cca ttc cac ctc ctg cca cag gta cgc aag gaa gtc gga tct gaa 1075Val Pro Phe His Leu Leu Pro Gln Val Arg Lys Glu Val Gly Ser Glu310 315 320 325cca acc atc atg atc gac acc ggc atc atg aac ggc gcc gac atc gtc 1123Pro Thr Ile Met Ile Asp Thr Gly Ile Met Asn Gly Ala Asp Ile Val
330 335 340gca gcc gta gcc atg ggc gct gac ttc acc ctc atc ggt cgt gcc tac 1171Ala Ala Val Ala Met Gly Ala Asp Phe Thr Leu Ile Gly Arg Ala Tyr
345 350 355ctc tac gga ctc atg gcc gga ggc cgc gaa ggc gtc gac cgc acc atc 1219Leu Tyr Gly Leu Met Ala Gly Gly Arg Glu Gly Val Asp Arg Thr Ile
360 365 370gcc att ctc cgc agc gag atc acc cgc acc atg gct ctc ctc ggt gtt 1267Ala Ile Leu Arg Ser Glu Ile Thr Arg Thr Met Ala Leu Leu Gly Val
375 380 385tcc tcc ctc gaa gaa ctc gag cca cgc cac gtc acc cag ctg gcc aag 1315Ser Ser Leu Glu Glu Leu Glu Pro Arg His Val Thr Gln Leu Ala Lys390 395 400 405atg gtt cca gtt tct gac gca act cgt tct gca gcg gcg gag att 1360Met Val Pro Val Ser Asp Ala Thr Arg Ser Ala Ala Ala Glu Ile
410 415 420taaaagtttc tctccttagc tat 1383<210>136<211>420<212>PRT<213>谷氨酸棒杆菌<400>136Met Val Lys Arg Gln Leu Pro Asn Pro Ala Glu Leu Leu Glu Leu Met1 5 10 15Lys Phe Lys Lys Pro Glu Leu Asn Gly Lys Lys Arg Arg Leu Asp Ser
20 25 30Ala Leu Thr Ile Tyr Asp Leu Arg Lys Ile Ala Lys Arg Arg Thr Pro
35 40 45Ala Ala Ala Phe Asp Tyr Thr Asp Gly Ala Ala Glu Ala Glu Leu Ser
50 55 60Ile Thr Arg Ala Arg Glu Ala Phe Glu Asn Ile Glu Phe His Pro Asp65 70 75 80Ile Leu Lys Pro Ala Glu His Val Asp Thr Thr Thr Gln Ile Leu Gly
85 90 95Gly Thr Ser Ser Met Pro Phe Gly Ile Ala Pro Thr Gly Phe Thr Arg
100 105 110Leu Met Gln Thr Glu Gly Glu Ile Ala Gly Ala Gly Ala Ala Gly Ala
115 120 125Ala Gly Ile Pro Phe Thr Leu Ser Thr Leu Gly Thr Thr Ser Ile Glu
130 135 140Asp Val Lys Ala Thr Asn Pro Asn Gly Arg Asn Trp Phe Gln Leu Tyr145 150 155 160Val Met Arg Asp Arg Glu Ile Ser Tyr Gly Leu Val Glu Arg Ala Ala
165 170 175Lys Ala Gly Phe Asp Thr Leu Met Phe Thr Val Asp Thr Pro Ile Ala
180 185 190Gly Tyr Arg Ile Arg Asp Ser Arg Asn Gly Phe Ser Ile Pro Pro Gln
195 200 205Leu Thr Pro Ser Thr Val Leu Asn Ala Ile Pro Arg Pro Trp Trp Trp
210 215 220Ile Asp Phe Leu Thr Thr Pro Thr Leu Glu Phe Ala Ser Leu Ser Ser225 230 235 240Thr Gly Gly Thr Val Gly Asp Leu Leu Asn Ser Ala Met Asp Pro Thr
245 250 255Ile Ser Tyr Glu Asp Leu Lys Val Ile Arg Glu Met Trp Pro Gly Lys
260 265 270Leu Val Val Lys Gly Val Gln Asn Val Glu Asp Ser Val Lys Leu Leu
275 280 285Asp Gln Gly Val Asp Gly Leu Ile Leu Ser Asn His Gly Gly Arg Gln
290 295 300Leu Asp Arg Ala Pro Val Pro Phe His Leu Leu Pro Gln Val Arg Lys305 310 315 320Glu Val Gly Ser Glu Pro Thr Ile Met Ile Asp Thr Gly Ile Met Asn
325 330 335Gly Ala Asp Ile Val Ala Ala Val Ala Met Gly Ala Asp Phe Thr Leu
340 345 350Ile Gly Arg Ala Tyr Leu Tyr Gly Leu Met Ala Gly Gly Arg Glu Gly
355 360 365Val Asp Arg Thr Ile Ala Ile Leu Arg Ser Glu Ile Thr Arg Thr Met
370 375 380Ala Leu Leu Gly Val Ser Ser Leu Glu Glu Leu Glu Pro Arg His Val385 390 395 400Thr Gln Leu Ala Lys Met Val Pro Val Ser Asp Ala Thr Arg Ser Ala
405 410 415Ala Ala Glu Ile
420<210>137<211>1836<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1813)<223>RXN01952<400>137ccatcaaaaa atgaacgacc gcggactagc tcggatcaag gcgacatccc ctcagcatca 60tgacgcgctt gtgatgcaac tgaatatagg aagcttagag atg acg caa cca gga 115
Met Thr Gln Pro Gly
1 5cag acc acc acg act tcg cac gaa gcg atc gat gcg ttc aag aga atc 163Gln Thr Thr Thr Thr Ser His Glu Ala Ile Asp Ala Phe Lys Arg Ile
10 15 20gtc ggc gac gaa cat gta ctg acc tct gag cgt gcc acg atg cca ttc 211Val Gly Asp Glu His Val Leu Thr Ser Glu Arg Ala Thr Met Pro Phe
25 30 35agc aaa ggc tat cga ttc ggc gga gga cca gtc ttc gcc gtg gtg cgc 259Ser Lys Gly Tyr Arg Phe Gly Gly Gly Pro Val Phe Ala Val Val Arg
40 45 50ccc ggc acg ctg gtc gag atg tgg cgg gcg ctg cag gta tcc gtc gac 307Pro Gly Thr Leu Val Glu Met Trp Arg Ala Leu Gln Val Ser Val Asp
55 60 65aac aac ctc atc gtc atc ccg cag gca tcg aac acg ggc ctg act ggt 355Asn Asn Leu Ile Val Ile Pro Gln Ala Ser Asn Thr Gly Leu Thr Gly70 75 80 85gga tcc ggc ccc ggc ttc caa gac tac gat cgc ccc att gtg atc atc 403Gly Ser Gly Pro Gly Phe Gln Asp Tyr Asp Arg Pro Ile Val Ile Ile
90 95 100tcg act cac cgc atc gat gag gtg cac ctc atc aac gac gcg cgc gag 451Ser Thr His Arg Ile Asp Glu Val His Leu Ile Asn Asp Ala Arg Glu
105 110 115gcg atc tcg ctc gcg ggc acc ccg ctg aca cac ctg acc gac gcg ctc 499Ala Ile Ser Leu Ala Gly Thr Pro Leu Thr His Leu Thr Asp Ala Leu
120 125 130gcc aag cac cag cgc gag ccg cac tcg gtg atc ggg tcg aca tca atc 547Ala Lys His Gln Arg Glu Pro His Ser Val Ile Gly Ser Thr Ser Ile
135 140 145ggc gcc tcg gtc atc ggc ggc atc gcg aac aac tcg ggc ggc agc cag 595Gly Ala Ser Val Ile Gly Gly Ile Ala Asn Asn Ser Gly Gly Ser Gln150 155 160 165att cgc aag ggt ccg gca ttc acg cgc gaa gcg atc ttc gcc cgc gtc 643Ile Arg Lys Gly Pro Ala Phe Thr Arg Glu Ala Ile Phe Ala Arg Val
170 175 180aac gac gac ggc aag gtc gag ctg gtc aat cac ctg ggc atc tcg ctc 691Asn Asp Asp Gly Lys Val Glu Leu Val Asn His Leu Gly Ile Ser Leu
185 190 195gga gac gac cct gag gtc gca ctc gac cgt cta cag cgc ggc gag tgg 739Gly Asp Asp Pro Glu Val Ala Leu Asp Arg Leu Gln Arg Gly Glu Trp
200 205 210tct ccc gag gat gtc acc cca gct ccc gaa gac tcg aac gag acc gag 787Ser Pro Glu Asp Val Thr Pro Ala Pro Glu Asp Ser Asn Glu Thr Glu
215 220 225tac gcc gag cac ttg cgc aag atc gtg cct tcg cct gct cgc tac aat 835Tyr AIa Glu His Leu Arg Lys Ile Val Pro Ser Pro Ala Arg Tyr Asn230 235 240 245gcg aac ccc gag tac ctg ttc gag gct tcc ggc tcg gcc ggc aag ctg 883Ala Asn Pro Glu Tyr Leu Phe Glu Ala Ser Gly Ser Ala Gly Lys Leu
250 255 260atg gtg ttc gcg gtg cgc acc cgc acc ttc cct cgc gaa gtg cac ccg 931Met Val Phe Ala Val Arg Thr Arg Thr Phe Pro Arg Glu Val His Pro
265 270 275acc gtg ttt tac atc ggc acg aac aac acg cac gag ctc gaa gag atc 979Thr Val Phe Tyr Ile Gly Thr Asn Asn Thr His Glu Leu Glu Glu Ile
280 285 290cgt cgg ttg ttc ctc gaa gcc gac atg ccg ctg cct atc tct ggt gag 1027Arg Arg Leu Phe Leu Glu Ala Asp Met Pro Leu Pro Ile Ser Gly Glu
295 300 305tac atg ggc cgc agt gcc ttc gac ttg gcc gag aag tac ggc aaa gac 1075Tyr Met Gly Arg Ser Ala Phe Asp Leu Ala Glu Lys Tyr Gly Lys Asp310 315 320 325acc ttc gtc ttc ctg aag ttc atg agt cca gcg ctg cag acg cgc atg 1123Thr Phe Val Phe Leu Lys Phe Met Ser Pro Ala Leu Gln Thr Arg Met
330 335 340ttc tcg ttc aag acg tgg gcc aac ggc ttg ttc tcg aag att ccc ggc 1171Phe Ser Phe Lys Thr Trp Ala Asn Gly Leu Phe Ser Lys Ile Pro Gly
345 350 355att ggt ccg acc ttc gcc gac acg gta tcg caa gcc atg ttc agc gtg 1219Ile Gly Pro Thr Phe Ala Asp Thr Val Ser Gln Ala Met Phe Ser Val
360 365 370ctg ccc aac cag ctg ccc aag cgc atg atg gag tac cgc aac cgt ttc 1267Leu Pro Asn Gln Leu Pro Lys Arg Met Met Glu Tyr Arg Asn Arg Phe
375 380 385gag cat cac ctg ctg ctc acc gtc agc gag tcg cag aag gcc gcg agc 1315Glu His His Leu Leu Leu Thr Val Ser Glu Ser Gln Lys Ala Ala Ser390 395 400 405gag aag atg ctc aag gag ttc ttc gca gag ccc gag cac act ggt gag 1363Glu Lys Met Leu Lys Glu Phe Phe Ala Glu Pro Glu His Thr Gly Glu
410 415 420ttc ttc atc tgc acg tct gat gaa gaa aag agc gcg tcg ctc aac cgg 1411Phe Phe Ile Cys Thr Ser Asp Glu Glu Lys Ser Ala Ser Leu Asn Arg
425 430 435ttc ggc gcg gcc agt gcc gcc act cgc tac gcc gcg ttg aag cgc cgg 1459Phe Gly Ala Ala Ser Ala Ala Thr Arg Tyr Ala Ala Leu Lys Arg Arg
440 445 450cac atc gca ggg ctc atc ccc atc gat gtg gcc ctg cgt cgc gac gat 1507His Ile Ala Gly Leu Ile Pro Ile Asp Val Ala Leu Arg Arg Asp Asp
455 460 465tgg aac tgg ctc gag gtg ctg ccg gag gag atc gac gac cag ctt gag 1555Trp Asn Trp Leu Glu Val Leu Pro Glu Glu Ile Asp Asp Gln Leu Glu470 475 480 485gtc aag gcg tat tac ggg cac ttc ttc tgc cat gtg atg cac cag gac 1603Val Lys Ala Tyr Tyr Gly His Phe Phe Cys His Val Met His Gln Asp
490 495 500tat gtc gcc aag cag ggc gtg gat ctc gag gcg ctg cac gac cgc atc 1651Tyr Val Ala Lys Gln Gly Val Asp Leu Glu Ala Leu His Asp Arg Ile
505 510 515cag cac ctg ctg gag gag cgc ggc gcg aag ctg ccc gcc gag cac aac 1699Gln His Leu Leu Glu Glu Arg Gly Ala Lys Leu Pro Ala Glu His Asn
520 525 530tac ggt cgc atg tac aag ctg ccg gag tcc atg gaa gag cac ttc aag 1747Tyr Gly Arg Met Tyr Lys Leu Pro Glu Ser Met Glu Glu His Phe Lys
535 540 545gag ctc gat ccg acg aat acg ttc aac gcc ggt atc ggc ggc acg tcg 1795Glu Leu Asp Pro Thr Asn Thr Phe Asn Ala Gly Ile Gly Gly Thr Ser550 555 560 565ccg cac aag gac tgg gcc taagtcccca aggtagcgcg acg 1836Pro His Lys Asp Trp Ala
570<210>138<211>571<212>PRT<213>谷氨酸棒杆菌<400>138Met Thr Gln Pro Gly Gln Thr Thr Thr Thr Ser His Glu Ala Ile Asp1 5 10 15Ala Phe Lys Arg Ile Val Gly Asp Glu His Val Leu Thr Ser Glu Arg
20 25 30Ala Thr Met Pro Phe Ser Lys Gly Tyr Arg Phe Gly Gly Gly Pro Val
35 40 45Phe Ala Val Val Arg Pro Gly Thr Leu Val Glu Met Trp Arg Ala Leu
50 55 60Gln Val Ser Val Asp Asn Asn Leu Ile Val Ile Pro Gln Ala Ser Asn65 70 75 80Thr Gly Leu Thr Gly Gly Ser Gly Pro Gly Phe Gln Asp Tyr Asp Arg
85 90 95Pro Ile Val Ile Ile Ser Thr His Arg Ile Asp Glu Val His Leu Ile
100 105 110Asn Asp Ala Arg Glu Ala Ile Ser Leu Ala Gly Thr Pro Leu Thr His
115 120 125Leu Thr Asp Ala Leu Ala Lys His Gln Arg Glu Pro His Ser Val Ile
130 135 140Gly Ser Thr Ser Ile Gly Ala Ser Val Ile Gly Gly Ile Ala Asn Asn145 150 155 160Ser Gly Gly Ser Gln Ile Arg Lys Gly Pro Ala Phe Thr Arg Glu Ala
165 170 175Ile Phe Ala Arg Val Asn Asp Asp Gly Lys Val Glu Leu Val Asn His
180 185 190Leu Gly Ile Ser Leu Gly Asp Asp Pro Glu Val Ala Leu Asp Arg Leu
195 200 205Gln Arg Gly Glu Trp Ser Pro Glu Asp Val Thr Pro Ala Pro Glu Asp
210 215 220Ser Asn Glu Thr Glu Tyr Ala Glu His Leu Arg Lys Ile Val Pro Ser225 230 235 240Pro Ala Arg Tyr Asn Ala Asn Pro Glu Tyr Leu Phe Glu Ala Ser Gly
245 250 255Ser Ala Gly Lys Leu Met Val Phe Ala Val Arg Thr Arg Thr Phe Pro
260 265 270Arg Glu Val His Pro Thr Val Phe Tyr Ile Gly Thr Asn Asn Thr His
275 280 285Glu Leu Glu Glu Ile Arg Arg Leu Phe Leu Glu Ala Asp Met Pro Leu
290 295 300Pro Ile Ser Gly Glu Tyr Met Gly Arg Ser Ala Phe Asp Leu Ala Glu305 310 315 320Lys Tyr Gly Lys Asp Thr Phe Val Phe Leu Lys Phe Met Ser Pro Ala
325 330 335Leu Gln Thr Arg Met Phe Ser Phe Lys Thr Trp Ala Asn Gly Leu Phe
340 345 350Ser Lys Ile Pro Gly Ile Gly Pro Thr Phe Ala Asp Thr Val Ser Gln
355 360 365Ala Met Phe Ser Val Leu Pro Asn Gln Leu Pro Lys Arg Met Met Glu
370 375 380Tyr Arg Asn Arg Phe Glu His His Leu Leu Leu Thr Val Ser Glu Ser385 390 395 400Gln Lys Ala Ala Ser Glu Lys Met Leu Lys Glu Phe Phe Ala Glu Pro
405 410 415Glu His Thr Gly Glu Phe Phe Ile Cys Thr Ser Asp Glu Glu Lys Ser
420 425 430Ala Ser Leu Asn Arg Phe Gly Ala Ala Ser Ala Ala Thr Arg Tyr Ala
435 440 445Ala Leu Lys Arg Arg His Ile Ala Gly Leu Ile Pro Ile Asp Val Ala
450 455 460Leu Arg Arg Asp Asp Trp Asn Trp Leu Glu Val Leu Pro Glu Glu Ile465 470 475 480Asp Asp Gln Leu Glu Val Lys Ala Tyr Tyr Gly His Phe Phe Cys His
485 490 495Val Met His Gln Asp Tyr Val Ala Lys Gln Gly Val Asp Leu Glu Ala
500 505 510Leu His Asp Arg Ile Gln His Leu Leu Glu Glu Arg Gly Ala Lys Leu
515 520 525Pro Ala Glu His Asn Tyr Gly Arg Met Tyr Lys Leu Pro Glu Ser Met
530 535 540Glu Glu His Phe Lys Glu Leu Asp Pro Thr Asn Thr Phe Asn Ala Gly545 550 555 560Ile Gly Gly Thr Ser Pro His Lys Asp Trp Ala
565 570<210>139<211>239<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(216)<223>FRXA01952<400>139cca gga cta tgt cgc caa gca ggg cgt gga tct caa ggc gct gac gac 48Pro Gly Leu Cys Arg Gln Ala Gly Arg Gly Ser Gln Gly Ala Asp Asp1 5 10 15cgc atc cag cac ttg ctg gag gag cac ggc aag aag ctg ccc gcc gag 96Arg Ile Gln His Leu Leu Glu Glu His Gly Lys Lys Leu Pro Ala Glu
20 25 30cac aac tac ggt cgc atg tac aag ctg ccg gag tcc atg gaa gag cac 144His Asn Tyr Gly Arg Met Tyr Lys Leu Pro Glu Ser Met Glu Glu His
35 40 45ttc aag gag ctc gat ccg acg aat acg ttc aac gcc ggt atc ggc ggc 192Phe Lys Glu Leu Asp Pro Thr Asn Thr Phe Asn Ala Gly Ile Gly Gly
50 55 60acg tcg ccg cac aag gac tgg gcc taagtcccca aggtagcgcg acg 239Thr Ser Pro His Lys Asp Trp Ala65 70<210>140<211>72<212>PRT<213>谷氨酸棒杆菌<400>140Pro Gly Leu Cys Arg Gln Ala Gly Arg Gly Ser Gln Gly Ala Asp Asp1 5 10 15Arg Ile Gln His Leu Leu Glu Glu His Gly Lys Lys Leu Pro Ala Glu
20 25 30His Asn Tyr Gly Arg Met Tyr Lys Leu Pro Glu Ser Met Glu Glu His
35 40 45Phe Lys Glu Leu Asp Pro Thr Asn Thr Phe Asn Ala Gly Ile Gly Gly
50 55 60Thr Ser Pro His Lys Asp Trp Ala65 70<210>141<211>1699<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1699)<223>FRXA01955<400>141ccatcaaaaa atgaacgacc gcggactagc tcggatcaag gcgacatccc ctcagcatca 60tgacgcgctt gtgatgcaac tgaatatagg aagcttagag atg acg caa cca gga 115
Met Thr Gln Pro Gly
1 5cag acc acc acg act tcg cac gaa gcg atc gat gcg ttc aag aga atc 163Gln Thr Thr Thr Thr Ser His Glu Ala Ile Asp Ala Phe Lys Arg Ile
10 15 20gtc ggc gac gaa cat gta ctg acc tct gag cgt gcc acg atg cca ttc 211Val Gly Asp Glu His Val Leu Thr Ser Glu Arg Ala Thr Met Pro Phe
25 30 35agc aaa ggc tat cga ttc ggc gga gga cca gtc ttc gcc gtg gtg cgc 259Ser Lys Gly Tyr Arg Phe Gly Gly Gly Pro Val Phe Ala Val Val Arg
40 45 50ccc ggc acg ctg gtc gag atg tgg cgg gcg ctg cag gta tcc gtc gac 307Pro Gly Thr Leu Val Glu Met Trp Arg Ala Leu Gln Val Ser Val Asp
55 60 65aac aac ctc atc gtc atc ccg cag gca tcg aac acg ggc ctg act ggt 355Asn Asn Leu Ile Val Ile Pro Gln Ala Ser Asn Thr Gly Leu Thr Gly70 75 80 85gga tcc ggc ccc ggc ttc caa gac tac gat cgc ccc att gtg atc atc 403Gly Ser Gly Pro Gly Phe Gln Asp Tyr Asp Arg Pro Ile Val Ile Ile
90 95 100tcg act cac cgc atc gat gag gtg cac ctc atc aac gac gcg cgc gag 451Ser Thr His Arg Ile Asp Glu Val His Leu Ile Asn Asp Ala Arg Glu
105 110 115gcg atc tcg ctc gcg ggc acc ccg ctg aca cac ctg acc gac gcg ctc 499Ala Ile Ser Leu Ala Gly Thr Pro Leu Thr His Leu Thr Asp Ala Leu
120 125 130gcc aag cac cag cgc gag ccg cac tcg gtg atc ggg tcg aca tca atc 547Ala Lys His Gln Arg Glu Pro His Ser Val Ile Gly Ser Thr Ser Ile
135 140 145ggc gcc tcg gtc atc ggc ggc atc gcg aac aac tcg ggc ggc agc cag 595Gly Ala Ser Val Ile Gly Gly Ile Ala Asn Asn Ser Gly Gly Ser Gln150 155 160 165att cgc aag ggt ccg gca ttc acg cgc gaa gcg atc ttc gcc cgc gtc 643Ile Arg Lys Gly Pro Ala Phe Thr Arg Glu Ala Ile Phe Ala Arg Val
170 175 180aac gac gac ggc aag gtc gag ctg gtc aat cac ctg ggc atc tcg ctc 691Asn Asp Asp Gly Lys Val Glu Leu Val Asn His Leu Gly Ile Ser Leu
185 190 195gga gac gac cct gag gtc gca ctc gac cgt cta cag cgc ggc gag tgg 739Gly Asp Asp Pro Glu Val Ala Leu Asp Arg Leu Gln Arg Gly Glu Trp
200 205 210tct ccc gag gat gtc acc cca gct ccc gaa gac tcg aac gag acc gag 787Ser Pro Glu Asp Val Thr Pro Ala Pro Glu Asp Ser Asn Glu Thr Glu
215 220 225tac gcc gag cac ttg cgc aag atc gtg cct tcg cct gct cgc tac aat 835Tyr Ala Glu His Leu Arg Lys Ile Val Pro Ser Pro Ala Arg Tyr Asn230 235 240 245gcg aac ccc gag tac ctg ttc gag gct tcc ggc tcg gcc ggc aag ctg 883Ala Asn Pro Glu Tyr Leu Phe Glu Ala Ser Gly Ser Ala Gly Lys Leu
250 255 260atg gtg ttc gcg gtg cgc acc cgc acc ttc cct cgc gaa gtg cac ccg 931Met Val Phe Ala Val Arg Thr Arg Thr Phe Pro Arg Glu Val His Pro
265 270 275acc gtg ttt tac atc ggc acg aac aac acg cac gag ctc gaa gag atc 979Thr Val Phe Tyr Ile Gly Thr Asn Aan Thr His Glu Leu Glu Glu Ile
280 285 290cgt cgg ttg ttc ctc gaa gcc gac atg ccg ctg cct atc tct ggt gag 1027Arg Arg Leu Phe Leu Glu Ala Asp Met Pro Leu Pro Ile Ser Gly Glu
295 300 305tac atg ggc cgc agt gcc ttc gac ttg gcc gag aag tac ggc aaa gac 1075Tyr Met Gly Arg Ser Ala Phe Asp Leu Ala Glu Lys Tyr Gly Lys Asp310 315 320 325acc ttc gtc ttc ctg aag ttc atg agt cca gcg ctg cag acg cgc atg 1123Thr Phe Val Phe Leu Lys Phe Met Ser Pro Ala Leu Gln Thr Arg Met
330 335 340ttc tcg ttc aag acg tgg gcc aac ggc ttg ttc tcg aag att ccc ggc 1171Phe Ser Phe Lys Thr Trp Ala Asn Gly Leu Phe Ser Lys Ile Pro Gly
345 350 355att ggt ccg acc ttc gcc gac acg gta tcg caa gcc atg ttc agc gtg 1219Ile Gly Pro Thr Phe Ala Asp Thr Val Ser Gln Ala Met Phe Ser Val
360 365 370ctg ccc aac cag ctg ccc aag cgc atg atg gag tac cgc aac cgt ttc 1267Leu Pro Asn Gln Leu Pro Lys Arg Met Met Glu Tyr Arg Asn Arg Phe
375 380 385gag cat cac ctg ctg ctc acc gtc agc gag tcg cag aag gcc gcg agc 1315Glu His His Leu Leu Leu Thr Val Ser Glu Ser Gln Lys Ala Ala Ser390 395 400 405gag aag atg ctc aag gag ttc ttc gca gag ccc gag cac act ggt gag 1363Glu Lys Met Leu Lys Glu Phe Phe Ala Glu Pro Glu His Thr Gly Glu
410 415 420ttc ttc atc tgc acg tct gat gaa gaa aag agc gcg tcg ctc aac cgg 1411Phe Phe Ile Cys Thr Ser Asp Glu Glu Lys Ser Ala Ser Leu Asn Arg
425 430 435ttc ggc gcg gcc agt gcc gcc act cgc tac gcc gcg ttg aag cgc cgg 1459Phe Gly Ala Ala Ser Ala Ala Thr Arg Tyr Ala Ala Leu Lys Arg Arg
440 445 450cac atc gca ggg ctc atc ccc atc gat gtg gcc ctg cgt cgc gac gat 1507His Ile Ala Gly Leu Ile Pro Ile Asp Val Ala Leu Arg Arg Asp Asp
455 460 465tgg aac tgg ctc gag gtg ctg ccg gag gag atc gac gac cag ctt gag 1555Trp Asn Trp Leu Glu Val Leu Pro Glu Glu Ile Asp Asp Gln Leu Glu470 475 480 485gtc aag gcg tat tac ggg cac ttc ttc tgc cat gtg atg cac cag gac 1603Val Lys Ala Tyr Tyr Gly His Phe Phe Cys His Val Met His Gln Asp
490 495 500tat gtc gcc aag cag ggc gtg gat ctc gag gcg ctg cac gac cgc atc 1651Tyr Val Ala Lys Gln Gly Val Asp Leu Glu Ala Leu His Asp Arg Ile
505 510 515cag cac ctg ctg gag gag cgc ggc gcg aag ctg ccc gcc gag cac aac 1699Gln His Leu Leu Glu Glu Arg Gly Ala Lys Leu Pro Ala Glu His Asn
520 525 530<210>142<211>533<212>PRT<213>谷氨酸棒杆菌<400>142Met Thr Gln Pro Gly Gln Thr Thr Thr Thr Ser His Glu Ala Ile Asp1 5 10 15Ala Phe Lys Arg Ile Val Gly Asp Glu His Val Leu Thr Ser Glu Arg
20 25 30Ala Thr Met Pro Phe Ser Lys Gly Tyr Arg Phe Gly Gly Gly Pro Val
35 40 45Phe Ala Val Val Arg Pro Gly Thr Leu Val Glu Met Trp Arg Ala Leu
50 55 60Gln Val Ser Val Asp Asn Asn Leu Ile Val Ile Pro Gln Ala Ser Asn65 70 75 80Thr Gly Leu Thr Gly Gly Ser Gly Pro Gly Phe Gln Asp Tyr Asp Arg
85 90 95Pro Ile Val Ile Ile Ser Thr His Arg Ile Asp Glu Val His Leu Ile
100 105 110Asn Asp Ala Arg Glu Ala Ile Ser Leu Ala Gly Thr Pro Leu Thr His
115 120 125Leu Thr Asp Ala Leu Ala Lys His Gln Arg Glu Pro His Ser Val Ile
130 135 140Gly Ser Thr Ser Ile Gly Ala Ser Val Ile Gly Gly Ile Ala Asn Asn145 150 155 160Ser Gly Gly Ser Gln Ile Arg Lys Gly Pro Ala Phe Thr Arg Glu Ala
165 170 175Ile Phe Ala Arg Val Asn Asp Asp Gly Lys Val Glu Leu Val Asn His
180 185 190Leu Gly Ile Ser Leu Gly Asp Asp Pro Glu Val Ala Leu Asp Arg Leu
195 200 205Gln Arg Gly Glu Trp Ser Pro Glu Asp Val Thr Pro Ala Pro Glu Asp
210 215 220Ser Asn Glu Thr Glu Tyr Ala Glu His Leu Arg Lys Ile Val Pro Ser225 230 235 240Pro Ala Arg Tyr Asn Ala Asn Pro Glu Tyr Leu Phe Glu Ala Ser Gly
245 250 255Ser Ala Gly Lys Leu Met Val Phe Ala Val Arg Thr Arg Thr Phe Pro
260 265 270Arg Glu Val His Pro Thr Val Phe Tyr Ile Gly Thr Asn Asn Thr His
275 280 285Glu Leu Glu Glu Ile Arg Arg Leu Phe Leu Glu Ala Asp Met Pro Leu
290 295 300Pro Ile Ser Gly Glu Tyr Met Gly Arg Ser Ala Phe Asp Leu Ala Glu305 310 315 320Lys Tyr Gly Lys Asp Thr Phe Val Phe Leu Lys Phe Met Ser Pro Ala
325 330 335Leu Gln Thr Arg Met Phe Ser Phe Lys Thr Trp Ala Asn Gly Leu Phe
340 345 350Ser Lys Ile Pro Gly Ile Gly Pro Thr Phe Ala Asp Thr Val Ser Gln
355 360 365Ala Met Phe Ser Val Leu Pro Asn Gln Leu Pro Lys Arg Met Met Glu
370 375 380Tyr Arg Asn Arg Phe Glu His His Leu Leu Leu Thr Val Ser Glu Ser385 390 395 400Gln Lys Ala Ala Ser Glu Lys Met Leu Lys Glu Phe Phe Ala Glu Pro
405 410 415Glu His Thr Gly Glu Phe Phe Ile Cys Thr Ser Asp Glu Glu Lys Ser
420 425 430Ala Ser Leu Asn Arg Phe Gly Ala Ala Ser Ala Ala Thr Arg Tyr Ala
435 440 445Ala Leu Lys Arg Arg His Ile Ala Gly Leu Ile Pro Ile Asp Val Ala
450 455 460Leu Arg Arg Asp Asp Trp Asn Trp Leu Glu Val Leu Pro Glu Glu Ile465 470 475 480Asp Asp Gln Leu Glu Val Lys Ala Tyr Tyr Gly His Phe Phe Cys His
485 490 495Val Met His Gln Asp Tyr Val Ala Lys Gln Gly Val Asp Leu Glu Ala
500 505 510Leu His Asp Arg Ile Gln His Leu Leu Glu Glu Arg Gly Ala Lys Leu
515 520 525Pro Ala Glu His Asn
530<210>143<211>1035<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1012)<223>RXA00293<400>143agagattgtt gattcggcac caactttacc cacgagggta gaaatgaagc attcaggatg 60acaaaaccca acctcacacc aacaacctat cctggagccc atg aaa atc ttt gtt 115
Met Lys Ile Phe Val
1 5ggt ttt ggc gat tat cca ctc acc acc aag gcc ctt aag gag gca ggc 163Gly Phe Gly Asp Tyr Pro Leu Thr Thr Lys Ala Leu Lys Glu Ala Gly
10 15 20gca gaa ata gtg gac tcc ctc gaa aaa gcc gag ggg ttt gtc ttc act 211Ala Glu Ile Val Asp Ser Leu Glu Lys Ala Glu Gly Phe Val Phe Thr
25 30 35caa aca cca ggc aca gaa ttt ccc cta ctt ccc gac gga gtg agg tgg 259Gln Thr Pro Gly Thr Glu Phe Pro Leu Leu Pro Asp Gly Val Arg Trp
40 45 50gtg caa ttt ccc aat gcg ggg ctc aac gca tat ttc aca gct ggg cag 307Val Gln Phe Pro Asn Ala Gly Leu Asn Ala Tyr Phe Thr Ala Gly Gln
55 60 65att gat gac aaa cgc cgg tgg tca aat gca tca ggg gtg tat ggc caa 355Ile Asp Asp Lys Arg Arg Trp Ser Asn Ala Ser Gly Val Tyr Gly Gln70 75 80 85cag gta gcc gaa gca gca atg gct ctt ttg ctg gga ctg att cat atg 403Gln Val Ala Glu Ala Ala Met Ala Leu Leu Leu Gly Leu Ile His Met
90 95 100cac ccc acc atg gtg cgt gcc gat agt tgg gca cca agc act caa ata 451His Pro Thr Met Val Arg Ala Asp Ser Trp Ala Pro Ser Thr Gln Ile
105 110 115gat cag cag acc aga tgg ctt gat ggt gca aca gtt gcc att gtg gga 499Asp Gln Gln Thr Arg Trp Leu Asp Gly Ala Thr Val Ala Ile Val Gly
120 125 130gct ggt gga atc ggt aaa cat ctg gca gcc atg ttg aaa cct ttt ggc 547Ala Gly Gly Ile Gly Lys His Leu Ala Ala Met Leu Lys Pro Phe Gly
135 140 145gca aag tct tta gca gta agc agg acc ggt aca ccc acc caa gat ttt 595Ala Lys Ser Leu Ala Val Ser Arg Thr Gly Thr Pro Thr Gln Asp Phe150 155 160 165gat gca acg gaa cct ata tcc aac ctg cac caa gta ctt gcc gac gcc 643Asp Ala Thr Glu Pro Ile Ser Asn Leu His Gln Val Leu Ala Asp Ala
170 175 180gac cat gtg gtg ttg tgc gta ccg ctt acc gca gac acc tat cat ctg 691Asp His Val Val Leu Cys Val Pro Leu Thr Ala Asp Thr Tyr His Leu
185 190 195atc gga aaa gca gag ctt aaa gca atg cag tcc act gca att ttg atc 739Ile Gly Lys Ala Glu Leu Lys Ala Met Gln Ser Thr Ala Ile Leu Ile
200 205 210aac gtg gct cgc gga gaa gta gta gat aca gaa gca tta gtt gac gcc 787Asn Val Ala Arg Gly Glu Val Val Asp Thr Glu Ala Leu Val Asp Ala
215 220 225tta gat gcc caa gaa ata tcc ggg gca ggc tta gat gtc acc gat cct 835Leu Asp Ala Gln Glu Ile Ser Gly Ala Gly Leu Asp Val Thr Asp Pro230 235 240 245gaa cca ttg ccg gac gat cat cca ctg tgg ggg cgc agc aat gtg atc 883Glu Pro Leu Pro Asp Asp His Pro Leu Trp Gly Arg Ser Asn Val Ile
250 255 260att acc ccg cac gta gcc aac acg ttg acc tcg atg gat cgc atg ctt 931Ile Thr Pro His Val Ala Asn Thr Leu Thr Ser Met Asp Arg Met Leu
265 270 275gcc cca gtg gtg gca gaa aac tac cga cgg ttt ctc gct gga gaa aag 979Ala Pro Val Val Ala Glu Asn Tyr Arg Arg Phe Leu Ala Gly Glu Lys
280 285 290atg ctg acc gag gtg gac atc cac aaa ggt tac tagagcagtg gctttgaata 1032Met Leu Thr Glu Val Asp Ile His Lys Gly Tyr
295 300tag 1035<210>144<211>304<212>PRT<213>谷氨酸棒杆菌<400>144Met Lys Ile Phe Val Gly Phe Gly Asp Tyr Pro Leu Thr Thr Lys Ala1 5 10 15Leu Lys Glu Ala Gly Ala Glu Ile Val Asp Ser Leu Glu Lys Ala Glu
20 25 30Gly Phe Val Phe Thr Gln Thr Pro Gly Thr Glu Phe Pro Leu Leu Pro
35 40 45Asp Gly Val Arg Trp Val Gln Phe Pro Asn Ala Gly Leu Asn Ala Tyr
50 55 60Phe Thr Ala Gly Gln Ile Asp Asp Lys Arg Arg Trp Ser Asn Ala Ser65 70 75 80Gly Val Tyr Gly Gln Gln Val Ala Glu Ala Ala Met Ala Leu Leu Leu
85 90 95Gly Leu Ile His Met His Pro Thr Met Val Arg Ala Asp Ser Trp Ala
100 105 110Pro Ser Thr Gln Ile Asp Gln Gln Thr Arg Trp Leu Asp Gly Ala Thr
115 120 125Val Ala Ile Val Gly Ala Gly Gly Ile Gly Lys His Leu Ala Ala Met
130 135 140Leu Lys Pro Phe Gly Ala Lys Ser Leu Ala Val Ser Arg Thr Gly Thr145 150 155 160Pro Thr Gln Asp Phe Asp Ala Thr Glu Pro Ile Ser Asn Leu His Gln
165 170 175Val Leu Ala Asp Ala Asp His Val Val Leu Cys Val Pro Leu Thr Ala
180 185 190Asp Thr Tyr His Leu Ile Gly Lys Ala Glu Leu Lys Ala Met Gln Ser
195 200 205Thr Ala Ile Leu Ile Asn Val Ala Arg Gly Glu Val Val Asp Thr Glu
210 215 220Ala Leu Val Asp Ala Leu Asp Ala Gln Glu Ile Ser Gly Ala Gly Leu225 230 235 240Asp Val Thr Asp Pro Glu Pro Leu Pro Asp Asp His Pro Leu Trp Gly
245 250 255Arg Ser Asn Val Ile Ile Thr Pro His Val Ala Asn Thr Leu Thr Ser
260 265 270Met Asp Arg Met Leu Ala Pro Val Val Ala Glu Asn Tyr Arg Arg Phe
275 280 285Leu Ala Gly Glu Lys Met Leu Thr Glu Val Asp Ile His Lys Gly Tyr
290 295 300<210>145<211>687<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(62)..(664)<223>RXN0ll30<400>145agttcgtggc ggatgctgtg aacgtttccg gtggtcgcgt gggcgaagag gttctgtgtg 60g atg gat ctg gct cgc aag ctt ggt ctt ctt gct ggc aag ctt gtc gac 109Met Asp Leu Ala Arg Lys Leu Gly Leu Leu Ala Gly Lys Leu Val Asp
1 5 10 15gcc gcc cca gtc tcc att gag gtt gag gct cga ggc gag ctt tct tcc 157Ala Ala Pro Val Ser Ile Glu Val Glu Ala Arg Gly Glu Leu Ser Ser
20 25 30gag cag gtc aat gca ctt ggt ttg tcc gct gtt cgt ggt ttg ttc tcc 205Glu Gln Val Asn Ala Leu Gly Leu Ser Ala Val Arg Gly Leu Phe Ser
35 40 45gga att atc gaa gag tcc gtt act ttc gtc aac gct cct cgc att gct 253Gly Ile Ile Glu Glu Ser Val Thr Phe Val Asn Ala Pro Arg Ile Ala
50 55 60gaa gag cgt ggc ctg gac atc tcc gtg aag acc aac tct gag tct gtt 301Glu Glu Arg Gly Leu Asp Ile Ser Val Lys Thr Asn Ser Glu Ser Val65 70 75 80act cac cgt tcc gtc ctg cag gtc aag gtc att act ggc agc ggc gcg 349Thr His Arg Ser Val Leu Gln Val Lys Val Ile Thr Gly Ser Gly Ala
85 90 95agc gca act gtt gtt ggt gcc ctg act ggt ctt gag cgc gtt gag aag 397Ser Ala Thr Val Val Gly Ala Leu Thr Gly Leu Glu Arg Val Glu Lys
100 105 110atc acc cgc atc aat ggc cgt ggc ctg gat ctg cgc gca gag ggt ctg 445Ile Thr Arg Ile Asn Gly Arg Gly Leu Asp Leu Arg Ala Glu Gly Leu
115 120 125aac ctc ttc ctg cag tac act gac gct cct ggt gca ctg ggt acc gtt 493Asn Leu Phe Leu Gln Tyr Thr Asp Ala Pro Gly Ala Leu Gly Thr Val
130 135 140ggt acc aag ctg ggt gct gct ggc atc aac atc gag gct gct gcg ttg 541Gly Thr Lys Leu Gly Ala Ala Gly Ile Asn Ile Glu Ala Ala Ala Leu145 150 155 160act cag gct gag aag ggt gac ggc gct gtc ctg atc ctg cgt gtt gag 589Thr Gln Ala Glu Lys Gly Asp Gly Ala Val Leu Ile Leu Arg Val Glu
165 170 175tcc gct gtc tct gaa gag ctg gaa gct gaa atc aac gct gag ttg ggt 637Ser Ala Val Ser Glu Glu Leu Glu Ala Glu Ile Asn Ala Glu Leu Gly
180 185 190gct act tcc ttc cag gtt gat ctt gac taattagaga tccatttgct tga 687Ala Thr Ser Phe Gln Val Asp Leu Asp
195 200<210>146<211>201<212>PRT<213>谷氨酸棒杆菌<400>146Met Asp Leu Ala Arg Lys Leu Gly Leu Leu Ala Gly Lys Leu Val Asp1 5 10 15Ala Ala Pro Val Ser Ile Glu Val Glu Ala Arg Gly Glu Leu Ser Ser
20 25 30Glu Gln Val Asn Ala Leu Gly Leu Ser Ala Val Arg Gly Leu Phe Ser
35 40 45Gly Ile Ile Glu Glu Ser Val Thr Phe Val Asn Ala Pro Arg Ile Ala
50 55 60Glu Glu Arg Gly Leu Asp Ile Ser Val Lys Thr Asn Ser Glu Ser Val65 70 75 80Thr His Arg Ser Val Leu Gln Val Lys Val Ile Thr Gly Ser Gly Ala
85 90 95Ser Ala Thr Val Val Gly Ala Leu Thr Gly Leu Glu Arg Val Glu Lys
100 105 110Ile Thr Arg Ile Asn Gly Arg Gly Leu Asp Leu Arg Ala Glu Gly Leu
115 120 125Asn Leu Phe Leu Gln Tyr Thr Asp Ala Pro Gly Ala Leu Gly Thr Val
130 135 140Gly Thr Lys Leu Gly Ala Ala Gly Ile Asn Ile Glu Ala Ala Ala Leu145 150 155 160Thr Gln Ala Glu Lys Gly Asp Gly Ala Val Leu Ile Leu Arg Val Glu
165 170 175Ser Ala Val Ser Glu Glu Leu Glu Ala Glu Ile Asn Ala Glu Leu Gly
180 185 190Ala Thr Ser Phe Gln Val Asp Leu Asp
195 200<210>147<211>326<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(303)<223>FRXA01130<400>147gtt ggt gcc ctg act ggt ctt gag cgc gtt gag aag atc acc cgc atc 48Val Gly Ala Leu Thr Gly Leu Glu Arg Val Glu Lys Ile Thr Arg Ile1 5 10 15aat ggc cgt ggc ctg gat ctg cgc gca gag ggt ctg aac ctc ttc ctg 96Asn Gly Arg Gly Leu Asp Leu Arg Ala Glu Gly Leu Asn Leu Phe Leu
20 25 30cag tac act gac gct cct ggt gca ctg ggt acc gtt ggt acc aag ctg 144Gln Tyr Thr Asp Ala Pro Gly Ala Leu Gly Thr Val Gly Thr Lys Leu
35 40 45ggt gct gct ggc atc aac atc gag gct gct gcg ttg act cag gct gag 192Gly Ala Ala Gly Ile Asn Ile Glu Ala Ala Ala Leu Thr Gln Ala Glu
50 55 60aag ggt gac ggc gct gtc ctg atc ctg cgt gtt gag tcc gct gtc tct 240Lys Gly Asp Gly Ala Val Leu Ile Leu Arg Val Glu Ser Ala Val Ser65 70 75 80gaa gag ctg gaa gct gaa atc aac gct gag ttg ggt gct act tcc ttc 288Glu Glu Leu Glu Ala Glu Ile Asn Ala Glu Leu Gly Ala Thr Ser Phe
85 90 95cag gtt gat ctt gac taattagaga tccatttgct tga 326Gln Val Asp Leu Asp
100<210>148<211>101<212>PRT<213>谷氨酸棒杆菌<400>148Val Gly Ala Leu Thr Gly Leu Glu Arg Val Glu Lys Ile Thr Arg Ile1 5 10 15Asn Gly Arg Gly Leu Asp Leu Arg Ala Glu Gly Leu Asn Leu Phe Leu
20 25 30Gln Tyr Thr Asp Ala Pro Gly Ala Leu Gly Thr Val Gly Thr Lys Leu
35 40 45Gly Ala Ala Gly Ile Asn Ile Glu Ala Ala Ala Leu Thr Gln Ala Glu
50 55 60Lys Gly Asp Gly Ala Val Leu Ile Leu Arg Val Glu Ser Ala Val Ser65 70 75 80Glu Glu Leu Glu Ala Glu Ile Asn Ala Glu Leu Gly Ala Thr Ser Phe
85 90 95Gln Val Asp Leu Asp
100<210>149<211>604<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(604)<223>RXN03112<400>149gtgagcacgc aatttctttg ctgctgtcta ctgctcgcca gatcctgctg ctgatgcgac 60gctgcgtgag ggcgagtgga agcggtcttc tttcaacggt gtg gaa att ttc gga 115
Val Glu Ile Phe Gly
1 5aaa act gtc ggt atc gtc ggt ttt ggc cac att ggt cag ttg ttt gct 163Lys Thr Val Gly Ile Val Gly Phe Gly His Ile Gly Gln Leu Phe Ala
10 15 20cag cgt ctt gct gcg ttt gag acc acc att gtt gct tac gat cct tac 211Gln Arg Leu Ala Ala Phe Glu Thr Thr Ile Val Ala Tyr Asp Pro Tyr
25 30 35gct aac cct gct cgt gcg gct cag ctg aac gtt gag ttg gtt gag ttg 259Ala Asn Pro Ala Arg Ala Ala Gln Leu Asn Val Glu Leu Val Glu Leu
40 45 50gat gag ctg atg agc cgt tct gac ttt gtc acc att cac ctt cct aag 307Asp Glu Leu Met Ser Arg Ser Asp Phe Val Thr Ile His Leu Pro Lys
55 60 65acc aag gaa act gct ggc atg ttt gat gcg cac ctc ctt gct aag tcc 355Thr Lys Glu Thr Ala Gly Met Phe Asp Ala His Leu Leu Ala Lys Ser70 75 80 85aag aag ggc cag atc atc atc aac gct gct cgt ggt ggc ctt gtt gat 403Lys Lys Gly Gln Ile Ile Ile Asn Ala Ala Arg Gly Gly Leu Val Asp
90 95 100gag cag gct ttg gct gat gcg att gag tcc ggt cac att cgt ggc gct 451Glu Gln Ala Leu Ala Asp Ala Ile Glu Ser Gly His Ile Arg Gly Ala
105 110 115ggt ttc gat gtg tac tcc acc gag cct tgc act gat tct cct ttg ttc 499Gly Phe Asp Val Tyr Ser Thr Glu Pro Cys Thr Asp Ser Pro Leu Phe
120 125 130aag ttg cct cag gtt gtt gtg act cct cac ttg ggt gct tct act gaa 547Lys Leu Pro Gln Val Val Val Thr Pro His Leu Gly Ala Ser Thr Glu
135 140 145gag gct caa gat cgt gcg ggt act gac att gct gat tct gtg ctc aag 595Glu Ala Gln Asp Arg Ala Gly Thr Asp Ile Ala Asp Ser Val Leu Lys150 155 160 165gcg ctg gct 604Ala Leu Ala<210>150<211>168<212>PRT<213>谷氨酸棒杆菌<400>150Val Glu Ile Phe Gly Lys Thr Val Gly Ile Val Gly Phe Gly His Ile1 5 10 15Gly Gln Leu Phe Ala Gln Arg Leu Ala Ala Phe Glu Thr Thr Ile Val
20 25 30Ala Tyr Asp Pro Tyr Ala Asn Pro Ala Arg Ala Ala Gln Leu Asn Val
35 40 45Glu Leu Val Glu Leu Asp Glu Leu Met Ser Arg Ser Asp Phe Val Thr
50 55 60Ile His Leu Pro Lys Thr Lys Glu Thr Ala Gly Met Phe Asp Ala His65 70 75 80Leu Leu Ala Lys Ser Lys Lys Gly Gln Ile Ile Ile Asn Ala Ala Arg
85 90 95Gly Gly Leu Val Asp Glu Gln Ala Leu Ala Asp Ala Ile Glu Ser Gly
100 105 110His Ile Arg Gly Ala Gly Phe Asp Val Tyr Ser Thr Glu Pro Cys Thr
115 120 125Asp Ser Pro Leu Phe Lys Leu Pro Gln Val Val Val Thr Pro His Leu
130 135 140Gly Ala Ser Thr Glu Glu Ala Gln Asp Arg Ala Gly Thr Asp Ile Ala145 150 155 160Asp Ser Val Leu Lys Ala Leu Ala
165<210>151<211>649<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(649)<223>FRXA01133<400>151tgtttctagt cgcacgccaa aacccggcgt ggacacgtct gcagccgacg cggtcgtgcc 60tgttgtagac ggacattcct agtttttcca ggagtaactt gtg agc cag aat ggc 115
Val Ser Gln Asn Gly
1 5cgt ccg gta gtc ctc atc gcc gat aag ctt gcg cag tcc act gtt gac 163Arg Pro Val Val Leu Ile Ala Asp Lys Leu Ala Gln Ser Thr Val Asp
10 15 20gcg ctt gga gat gca gta gaa gtc cgt tgg gtt gac gga cct aac cgc 211Ala Leu Gly Asp Ala Val Glu Val Arg Trp Val Asp Gly Pro Asn Arg
25 30 35cca gaa ctg ctt gat gca gtt aag gaa gcg gac gca ctg ctc gtg cgt 259Pro Glu Leu Leu Asp Ala Val Lys Glu Ala Asp Ala Leu Leu Val Arg
40 45 50tct gct acc act gtc gat gct gaa gtc atc gcc gct gcc cct aac ttg 307Ser Ala Thr Thr Val Asp Ala Glu Val Ile Ala Ala Ala Pro Asn Leu
55 60 65aag atc gtc ggt cgt gcc ggc gtg ggc ttg gac aac gtt gac atc cct 355Lys Ile Val Gly Arg Ala Gly Val Gly Leu Asp Asn Val Asp Ile Pro70 75 80 85gct gcc act gaa gct ggc gtc atg gtt gct aac gca ccg acc tct aat 403Ala Ala Thr Glu Ala Gly Val Met Val Ala Asn Ala Pro Thr Ser Asn
90 95 100att cac tcc gct tgt gag cac gca att tct ttg ctg ctg tct act gct 451Ile His Ser Ala Cys Glu His Ala Ile Ser Leu Leu Leu Ser Thr Ala
105 110 115cgc cag atc ctg ctg ctg atg cga cgc tgc gtg agg gcg agt gga agc 499Arg Gln Ile Leu Leu Leu Met Arg Arg Cys Val Arg Ala Ser Gly Ser
120 125 130ggt ctt ctt tca acg gtg tgg aaa ttt tcg gaa aaa ctg tcg gta tcg 547Gly Leu Leu Ser Thr Val Trp Lys Phe Ser Glu Lys Leu Ser Val Ser
135 140 145tcg gtt ttg gcc aca ttg gtc agt tgt ttg ctc agc gtc ttg ctg cgt 595Ser Val Leu Ala Thr Leu Val Ser Cys Leu Leu Ser Val Leu Leu Arg150 155 160 165ttg aga cca cca ttg ttg ctt acg atc ctt acg cta acc ctg ctt cgt 643Leu Arg Pro Pro Leu Leu Leu Thr Ile Leu Thr Leu Thr Leu Leu Arg
170 175 180gcg ggt 649Ala Gly<210>152<211>183<212>PRT<213>谷氨酸棒杆菌<400>152Val Ser Gln Asn Gly Arg Pro Val Val Leu Ile Ala Asp Lys Leu Ala1 5 10 15Gln Ser Thr Val Asp Ala Leu Gly Asp Ala Val Glu Val Arg Trp Val
20 25 30Asp Gly Pro Asn Arg Pro Glu Leu Leu Asp Ala Val Lys Glu Ala Asp
35 40 45Ala Leu Leu Val Arg Ser Ala Thr Thr Val Asp Ala Glu Val Ile Ala
50 55 60Ala Ala Pro Asn Leu Lys Ile Val Gly Arg Ala Gly Val Gly Leu Asp65 70 75 80Asn Val Asp Ile Pro Ala Ala Thr Glu Ala Gly Val Met Val Ala Asn
85 90 95Ala Pro Thr Ser Asn Ile His Ser Ala Cys Glu His Ala Ile Ser Leu
100 105 110Leu Leu Ser Thr Ala Arg Gln Ile Leu Leu Leu Met Arg Arg Cys Val
115 120 125Arg Ala Ser Gly Ser Gly Leu Leu Ser Thr Val Trp Lys Phe Ser Glu
130 135 140Lys Leu Ser Val Ser Ser Val Leu Ala Thr Leu Val Ser Cys Leu Leu145 150 155 160Ser Val Leu Leu Arg Leu Arg Pro Pro Leu Leu Leu Thr Ile Leu Thr
165 170 175Leu Thr Leu Leu Arg Ala Gly
180<210>153<211>1011<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(988)<223>RXN00871<400>153gggaaaaggc gatcaccagc cgttggctcg acccagcaac ccacggtggc attaacctcg 60gtttcccaca gaacgattaa ttgaaggaga gcacaggact atg cgt tgg ttc cat 115
Met Arg Trp Phe His
1 5aag aag ggc gaa ctg gcc cga gat ggt tgg caa agc gtt gtc gat gcc 163Lys Lys Gly Glu Leu Ala Arg Asp Gly Trp Gln Ser Val Val Asp Ala
10 15 20acc acc cca ggt tgg gaa tat acc ggc atc cgc att gcc gaa ctg ggc 211Thr Thr Pro Gly Trp Glu Tyr Thr Gly Ile Arg Ile Ala Glu Leu Gly
25 30 35agt ggt gaa tcg ctt gaa ctg aat gac act ggt gtg gaa cgc atc ttc 259Ser Gly Glu Ser Leu Glu Leu Asn Asp Thr Gly Val Glu Arg Ile Phe
40 45 50att cca ctt cag ggc agc ttc gat gtt gcc cac cat ggt cag gtg acc 307Ile Pro Leu Gln Gly Ser Phe Asp Val Ala His His Gly Gln Val Thr
55 60 65cat ctt cac gga aga aag tca gtc ttt gat gga cca acc gat gtg ctc 355His Leu His Gly Arg Lys Ser Val Phe Asp Gly Pro Thr Asp Val Leu70 75 80 85tac ctc ccc act gga caa aca gca acg ctc agt ggt cag gga cga gtc 403Tyr Leu Pro Thr Gly Gln Thr Ala Thr Leu Ser Gly Gln Gly Arg Val
90 95 100gcc gtg gcg gaa gct ccc act cag gaa ccc aag gag tgg aag tac atc 451Ala Val Ala Glu Ala Pro Thr Gln Glu Pro Lys Glu Trp Lys Tyr Ile
105 110 115gct cca gca gaa act cct gtg gag ttg cgt gga gct ggc cgc tcg agc 499Ala Pro Ala Glu Thr Pro Val Glu Leu Arg Gly Ala Gly Arg Ser Ser
120 125 130cga caa gtc cac aac ttt ggc acc ccg gaa gct ctc gat gct gct cga 547Arg Gln Val His Asn Phe Gly Thr Pro Glu Ala Leu Asp Ala Ala Arg
135 140 145cta atc gtg tgt gaa gta atc acc cca ggt gaa aac tgg agc tct tac 595Leu Ile Val Cys Glu Val Ile Thr Pro Gly Glu Asn Trp Ser Ser Tyr150 155 160 165cct cca cac aag cat gat gag cac atc cca gga cac gag tcc aag ctg 643Pro Pro His Lys His Asp Glu His Ile Pro Gly His Glu Ser Lys Leu
170 175 180gag gaa atc tac tac ttc gaa agc gcc cca tcg cga gtt ggt ggc agg 691Glu Glu Ile Tyr Tyr Phe Glu Ser Ala Pro Ser Arg Val Gly Gly Arg
185 190 195gcc gaa gca gca gaa gga gct ttc gga atg ttt tcc acc tac tcc tca 739Ala Glu Ala Ala Glu Gly Ala Phe Gly Met Phe Ser Thr Tyr Ser Ser
200 205 210cca gcg ggg gag atc gat atc aac gcc atg gtg tac agc ggc gat atc 787Pro Ala Gly Glu Ile Asp Ile Asn Ala Met Val Tyr Ser Gly Asp Ile
215 220 225gcg cta gtt cct ttc gga tac cac ggc cct gcc gtg gca gca cct ggc 835Ala Leu Val Pro Phe Gly Tyr His Gly Pro Ala Val Ala Ala Pro Gly230 235 240 245tat gac ttg tac tac ctc aac gtc atg gca gga cct gat ccg gag aga 883Tyr Asp Leu Tyr Tyr Leu Asn Val Met Ala Gly Pro Asp Pro Glu Arg
250 255 260atc tgg ctg att aac gat gac cca gcg cac gcc tgg gtt cga gat aca 931Ile Trp Leu Ile Asn Asp Asp Pro Ala His Ala Trp Val Arg Asp Thr
265 270 275tgg acc ggg caa gca ttt gat gat cgc ttg cca tat gag aac gca aac 979Trp Thr Gly Gln Ala Phe Asp Asp Arg Leu Pro Tyr Glu Asn Ala Asn
280 285 290aag gag gga taaaatttca tggctgaaac gaa 1011Lys Glu Gly
295<210>154<211>296<212>PRT<213>谷氨酸棒杆菌<400>154Met Arg Trp Phe His Lys Lys Gly Glu Leu Ala Arg Asp Gly Trp Gln1 5 10 15Ser Val Val Asp Ala Thr Thr Pro Gly Trp Glu Tyr Thr Gly Ile Arg
20 25 30Ile Ala Glu Leu Gly Ser Gly Glu Ser Leu Glu Leu Asn Asp Thr Gly
35 40 45Val Glu Arg Ile Phe Ile Pro Leu Gln Gly Ser Phe Asp Val Ala His
50 55 60His Gly Gln Val Thr His Leu His Gly Arg Lys Ser Val Phe Asp Gly65 70 75 80Pro Thr Asp Val Leu Tyr Leu Pro Thr Gly Gln Thr Ala Thr Leu Ser
85 90 95Gly Gln Gly Arg Val Ala Val Ala Glu Ala Pro Thr Gln Glu Pro Lys
100 105 110Glu Trp Lys Tyr Ile Ala Pro Ala Glu Thr Pro Val Glu Leu Arg Gly
115 120 125Ala Gly Arg Ser Ser Arg Gln Val His Asn Phe Gly Thr Pro Glu Ala
130 135 140Leu Asp Ala Ala Arg Leu Ile Val Cys Glu Val Ile Thr Pro Gly Glu145 150 155 160Asn Trp Ser Ser Tyr Pro Pro His Lys His Asp Glu His Ile Pro Gly
165 170 175His Glu Ser Lys Leu Glu Glu Ile Tyr Tyr Phe Glu Ser Ala Pro Ser
180 185 190Arg Val Gly Gly Arg Ala Glu Ala Ala Glu Gly Ala Phe Gly Met Phe
195 200 205Ser Thr Tyr Ser Ser Pro Ala Gly Glu Ile Asp Ile Asn Ala Met Val
210 215 220Tyr Ser Gly Asp Ile Ala Leu Val Pro Phe Gly Tyr His Gly Pro Ala225 230 235 240Val Ala Ala Pro Gly Tyr Asp Leu Tyr Tyr Leu Asn Val Met Ala Gly
245 250 255Pro Asp Pro Glu Arg Ile Trp Leu Ile Asn Asp Asp Pro Ala His Ala
260 265 270Trp Val Arg Asp Thr Trp Thr Gly Gln Ala Phe Asp Asp Arg Leu Pro
275 280 285Tyr Glu Asn Ala Asn Lys Glu Gly
290 295<210>155<211>964<212>DNA<213>谷氨酸棒杆菌<220><22l>CDS<222>(101)..(964)<223>FRXA00871<400>155gggaaaaggc gatcaccagc cgttggctcg acccagcaac ccacggtggc attaacctcg 60gtttcccaca gaacgattaa ttgaaggaga gcacaggact atg cgt tgg ttc cat 115
Met Arg Trp Phe His
1 5aag aag ggc gaa ctg gcc cga gat ggt tgg caa agc gtt gtc gat gcc 163Lys Lys Gly Glu Leu Ala Arg Asp Gly Trp Gln Ser Val Val Asp Ala
10 15 20acc acc cca ggt tgg gaa tat acc ggc atc cgc att gcc gaa ctg ggc 211Thr Thr Pro Gly Trp Glu Tyr Thr Gly Ile Arg Ile Ala Glu Leu Gly
25 30 35agt ggt gaa tcg ctt gaa ctg aat gac act ggt gtg gaa cgc atc ttc 259Ser Gly Glu Ser Leu Glu Leu Asn Asp Thr Gly Val Glu Arg Ile Phe
40 45 50att cca ctt cag ggc agc ttc gat gtt gcc cac cat ggt cag gtg acc 307Ile Pro Leu Gln Gly Ser Phe Asp Val Ala His His Gly Gln Val Thr
55 60 65cat ctt cac gga aga aag tca gtc ttt gat gga cca acc gat gtg ctc 355His Leu His Gly Arg Lys Ser Val Phe Asp Gly Pro Thr Asp Val Leu70 75 80 85tac ctc ccc act gga caa aca gca acg ctc agt ggt cag gga cga gtc 403Tyr Leu Pro Thr Gly Gln Thr Ala Thr Leu Ser Gly Gln Gly Arg Val
90 95 100gcc gtg gcg gaa gct ccc act cag gaa ccc aag gag tgg aag tac atc 451Ala Val Ala Glu Ala Pro Thr Gln Glu Pro Lys Glu Trp Lys Tyr Ile
105 110 115gct cca gca gaa act cct gtg gag ttg cgt gga gct ggc cgc tcg agc 499Ala Pro Ala Glu Thr Pro Val Glu Leu Arg Gly Ala Gly Arg Ser Ser
120 125 130cga caa gtc cac aac ttt ggc acc ccg gaa gct ctc gat gct gct cga 547Arg Gln Val His Asn Phe Gly Thr Pro Glu Ala Leu Asp Ala Ala Arg
135 140 145cta atc gtg tgt gaa gta atc acc cca ggt gaa aac tgg agc tct tac 595Leu Ile Val Cys Glu Val Ile Thr Pro Gly Glu Asn Trp Ser Ser Tyr150 155 160 165cct cca cac aag cat gat gag cac atc cca gga cac gag tcc aag ctg 643Pro Pro His Lys His Asp Glu His Ile Pro Gly His Glu Ser Lys Leu
170 175 180gag gaa atc tac tac ttc gaa agc gcc cca tcg cga gtt ggt ggc agg 691Glu Glu Ile Tyr Tyr Phe Glu Ser Ala Pro Ser Arg Val Gly Gly Arg
185 190 195gcc gaa gca gca gaa gga gct ttc gga atg ttt tcc acc tac tcc tca 739Ala Glu Ala Ala Glu Gly Ala Phe Gly Met Phe Ser Thr Tyr Ser Ser
200 205 210cca gcg ggg gag atc gat atc aac gcc atg gtg tac agc ggc gat atc 787Pro Ala Gly Glu Ile Asp Ile Asn Ala Met Val Tyr Ser Gly Asp Ile
215 220 225gcg cta gtt cct ttc gga tac cac ggc cct gcc gtg gca gca cct ggc 835Ala Leu Val Pro Phe Gly Tyr His Gly Pro Ala Val Ala Ala Pro Gly230 235 240 245tat gac ttg tac tac ctc aac gtc atg gca gga cct gat ccg gag aga 883Tyr Asp Leu Tyr Tyr Leu Asn Val Met Ala Gly Pro Asp Pro Glu Arg
250 255 260atc tgg ctg att aac gat gac cca gcg cac gcc tgg gtt cga gat aca 931Ile Trp Leu Ile Asn Asp Asp Pro Ala His Ala Trp Val Arg Asp Thr
265 270 275tgg acc ggg caa gca ttt gat gat cgc ttg cca 964Trp Thr Gly Gln Ala Phe Asp Asp Arg Leu Pro
280 285<210>156<211>288<212>PRT<213>谷氨酸棒杆菌<400>156Met Arg Trp Phe His Lys Lys Gly Glu Leu Ala Arg Asp Gly Trp Gln1 5 10 15Ser Val Val Asp Ala Thr Thr Pro Gly Trp Glu Tyr Thr Gly Ile Arg
20 25 30Ile Ala Glu Leu Gly Ser Gly Glu Ser Leu Glu Leu Asn Asp Thr Gly
35 40 45Val Glu Arg Ile Phe Ile Pro Leu Gln Gly Ser Phe Asp Val Ala His
50 55 60His Gly Gln Val Thr His Leu His Gly Arg Lys Ser Val Phe Asp Gly65 70 75 80Pro Thr Asp Val Leu Tyr Leu Pro Thr Gly Gln Thr Ala Thr Leu Ser
85 90 95Gly Gln Gly Arg Val Ala Val Ala Glu Ala Pro Thr Gln Glu Pro Lys
100 105 110Glu Trp Lys Tyr Ile Ala Pro Ala Glu Thr Pro Val Glu Leu Arg Gly
115 120 125Ala Gly Arg Ser Ser Arg Gln Val His Asn Phe Gly Thr Pro Glu Ala
130 135 140Leu Asp Ala Ala Arg Leu Ile Val Cys Glu Val Ile Thr Pro Gly Glu145 150 155 160Asn Trp Ser Ser Tyr Pro Pro His Lys His Asp Glu His Ile Pro Gly
165 170 175His Glu Ser Lys Leu Glu Glu Ile Tyr Tyr Phe Glu Ser Ala Pro Ser
180 185 190Arg Val Gly Gly Arg Ala Glu Ala Ala Glu Gly Ala Phe Gly Met Phe
195 200 205Ser Thr Tyr Ser Ser Pro Ala Gly Glu Ile Asp Ile Asn Ala Met Val
210 215 220Tyr Ser Gly Asp Ile Ala Leu Val Pro Phe Gly Tyr His Gly Pro Ala225 230 235 240Val Ala Ala Pro Gly Tyr Asp Leu Tyr Tyr Leu Asn Val Met Ala Gly
245 250 255Pro Asp Pro Glu Arg Ile Trp Leu Ile Asn Asp Asp Pro Ala His Ala
260 265 270Trp Val Arg Asp Thr Trp Thr Gly Gln Ala Phe Asp Asp Arg Leu Pro
275 280 285<210>157<211>373<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(373)<223>RXN02829<400>157tttttcgttt aatctcatat ttaaacacgt tccttttaat tggttttata aattgataaa 60ctgaattcgt cagttaaagt gtatcgaaag gagactggac atg caa aaa aat att 115
Met Gln Lys Asn Ile
1 5cta aaa agt ggc atc gaa att tct gaa ctt ggg tta ggt tgc atg agt 163Leu Lys Ser Gly Ile Glu Ile Ser Glu Leu Gly Leu Gly Cys Met Ser
10 15 20tta ggc aca gat tat aaa aaa gcg caa cca att att gaa agt gca att 211Leu Gly Thr Asp Tyr Lys Lys Ala Gln Pro Ile Ile Glu Ser Ala Ile
25 30 35gat aat ggt att acg tat ttt gat act gca gat att tac gat caa gga 259Asp Asn Gly Ile Thr Tyr Phe Asp Thr Ala Asp Ile Tyr Asp Gln Gly
40 45 50gtt aat gaa gaa att gtt ggt aaa gcc tta aaa aaa tat caa aat cgt 307Val Asn Glu Glu Ile Val Gly Lys Ala Leu Lys Lys Tyr Gln Asn Arg
55 60 65gat gac atc gtt atc gga act aaa gtt gga aat cga tta act gac gat 355Asp Asp Ile Val Ile Gly Thr Lys Val Gly Asn Arg Leu Thr Asp Asp70 75 80 85gga cat atg acg tgg gat 373Gly His Met Thr Trp Asp
90<210>158<211>91<212>PRT<213>谷氨酸棒杆菌<400>158Met Gln Lys Asn Ile Leu Lys Ser Gly Ile Glu Ile Ser Glu Leu Gly1 5 10 15Leu Gly Cys Met Ser Leu Gly Thr Asp Tyr Lys Lys Ala Gln Pro Ile
20 25 30Ile Glu Ser Ala Ile Asp Asn Gly Ile Thr Tyr Phe Asp Thr Ala Asp
35 40 45Ile Tyr Asp Gln Gly Val Asn Glu Glu Ile Val Gly Lys Ala Leu Lys
50 55 60Lys Tyr Gln Asn Arg Asp Asp Ile Val Ile Gly Thr Lys Val Gly Asn65 70 75 80Arg Leu Thr Asp Asp Gly His Met Thr Trp Asp
85 90<210>159<211>376<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(376)<223>FRXA02829<400>159tttttcgttt aatctcatat ttaaacacgt tccttttaat tggttttata aattgataaa 60ctgaattcgt cagttaaagt gtatcgaaag gagactggac atg caa aaa aat att 115
Met Gln Lys Asn Ile
1 5cta aaa agt ggc atc gaa att tct gaa ctt ggg tta ggt tgc atg agt 163Leu Lys Ser Gly Ile Glu Ile Ser Glu Leu Gly Leu Gly Cys Met Ser
10 15 20tta ggc aca gat tat aaa aaa gcg caa cca att att gaa agt gca att 211Leu Gly Thr Asp Tyr Lys Lys Ala Gln Pro Ile Ile Glu Ser Ala Ile
25 30 35gat aat ggt att acg tat ttt gat act gca gat att tac gat caa gga 259Asp Asn Gly Ile Thr Tyr Phe Asp Thr Ala Asp Ile Tyr Asp Gln Gly
40 45 50gtt aat gaa gaa att gtt ggt aaa gcc tta aaa aaa tat caa aat cgt 307Val Asn Glu Glu Ile Val Gly Lys Ala Leu Lys Lys Tyr Gln Asn Arg
55 60 65gat gac atc gtt atc gga act aaa gtt gga aat cga tta act gac gat 355Asp Asp Ile Val Ile Gly Thr Lys Val Gly Asn Arg Leu Thr Asp Asp70 75 80 85gga cat atg acg tgg gga tcc 376Gly His Met Thr Trp Gly Ser
90<210>160<211>92<212>PRT<213>谷氨酸棒杆菌<400>160Met Gln Lys Asn Ile Leu Lys Ser Gly Ile Glu Ile Ser Glu Leu Gly1 5 10 15Leu Gly Cys Met Ser Leu Gly Thr Asp Tyr Lys Lys Ala Gln Pro Ile
20 25 30Ile Glu Ser Ala Ile Asp Asn Gly Ile Thr Tyr Phe Asp Thr Ala Asp
35 40 45Ile Tyr Asp Gln Gly Val Asn Glu Glu Ile Val Gly Lys Ala Leu Lys
50 55 60Lys Tyr Gln Asn Arg Asp Asp Ile Val Ile Gly Thr Lys Val Gly Asn65 70 75 80Arg Leu Thr Asp Asp Gly His Met Thr Trp Gly Ser
85 90<210>161<211>948<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(925)<223>RXN01468<400>161tgccaaggat ttgaccaccg tgcaggattt gattgacttt attaacacca ataaggctga 60ttagcgggaa aatttcgccc aaaacaggga caatggtgtt atg aca gtg aac att 115
Met Thr Val Asn Ile
1 5tca tat ctg acc gac atg gac ggc gtc ctc atc aaa gag ggc gag ata 163Ser Tyr Leu Thr Asp Met Asp Gly Val Leu Ile Lys Glu Gly Glu Ile
10 15 20att ccg ggt gca gat cgt ttt ctt cag tct ctc acc gat aac aat gtg 211Ile Pro Gly Ala Asp Arg Phe Leu Gln Ser Leu Thr Asp Asn Asn Val
25 30 35gag ttt atg gtt ttg acc aac aac tcc att ttc acc ccg agg gat ctt 259Glu Phe Met Val Leu Thr Asn Asn Ser Ile Phe Thr Pro Arg Asp Leu
40 45 50tct gca cgt ctt aag act tcc ggt ttg gat atc ccg ccg gag cgt att 307Ser Ala Arg Leu Lys Thr Ser Gly Leu Asp Ile Pro Pro Glu Arg Ile
55 60 65tgg act tct gca acc gcc act gct cac ttc ctg aaa tcc cag gtc aag 355Trp Thr Ser Ala Thr Ala Thr Ala His Phe Leu Lys Ser Gln Val Lys70 75 80 85gag ggc aca gcc tat gtt gtt ggc gag tcc ggt ctg acc act gcg ttg 403Glu Gly Thr Ala Tyr Val Val Gly Glu Ser Gly Leu Thr Thr Ala Leu
90 95 100cat acc gcg ggt tgg att ttg acg gat gca aat cct gag ttt gtt gtc 451His Thr Ala Gly Trp Ile Leu Thr Asp Ala Asn Pro Glu Phe Val Val
105 110 115ctg ggc gaa acc cgc aca tat tcc ttc gag gca atc act act gcg ata 499Leu Gly Glu Thr Arg Thr Tyr Ser Phe Glu Ala Ile Thr Thr Ala Ile
120 125 130aat ctg att ttg ggt ggc gct cgc ttt att tgc acc aac ccg gat gtc 547Asn Leu Ile Leu Gly Gly Ala Arg Phe Ile Cys Thr Asn Pro Asp Val
135 140 145act gga cct tca cca agt ggc att ttg cct gct act ggc tct gtc gcc 595Thr Gly Pro Ser Pro Ser Gly Ile Leu Pro Ala Thr Gly Ser Val Ala150 155 160 165gca ctt att acc gca gct act ggc gct gag cct tat tac atc ggc aag 643Ala Leu Ile Thr Ala Ala Thr Gly Ala Glu Pro Tyr Tyr Ile Gly Lys
170 175 180cca aac cct gtg atg atg cgc agt gcg ctg aac acc atc ggg gcg cat 691Pro Asn Pro Val Met Met Arg Ser Ala Leu Asn Thr Ile Gly Ala His
185 190 195tcc gag cac act gtc atg atc ggc gac cgc atg gac acc gac gtg aaa 739Ser Glu His Thr Val Met Ile Gly Asp Arg Met Asp Thr Asp Val Lys
200 205 210tct ggt ttg gaa gcc ggc ctg agc acc gtg ctg gtt cga agc gga att 787Ser Gly Leu Glu Ala Gly Leu Ser Thr Val Leu Val Arg Ser Gly Ile
215 220 225tcc gac gac gcc gag atc cgc cgc tac ccc ttc cgc cca act cac gtg 835Ser Asp Asp Ala Glu Ile Arg Arg Tyr Pro Phe Arg Pro Thr His Val230 235 240 245atc aat tcc atc gcc gat ctt gcc gat tgc tgg gac gat cct ttc ggt 883Ile Asn Ser Ile Ala Asp Leu Ala Asp Cys Trp Asp Asp Pro Phe Gly
250 255 260gac ggt gca ttt cac gta cca gat gag cag cag ttc act gac 925Asp Gly Ala Phe His Val Pro Asp Glu Gln Gln Phe Thr Asp
265 270 275tagtattctg taggtcatgg cat 948<210>162<211>275<212>PRT<213>谷氨酸棒杆菌<400>162Met Thr Val Asn Ile Ser Tyr Leu Thr Asp Met Asp Gly Val Leu Ile1 5 10 15Lys Glu Gly Glu Ile Ile Pro Gly Ala Asp Arg Phe Leu Gln Ser Leu
20 25 30Thr Asp Asn Asn Val Glu Phe Met Val Leu Thr Asn Asn Ser Ile Phe
35 40 45Thr Pro Arg Asp Leu Ser Ala Arg Leu Lys Thr Ser Gly Leu Asp Ile
50 55 60Pro Pro Glu Arg Ile Trp Thr Ser Ala Thr Ala Thr Ala His Phe Leu65 70 75 80Lys Ser Gln Val Lys Glu Gly Thr Ala Tyr Val Val Gly Glu Ser Gly
85 90 95Leu Thr Thr Ala Leu His Thr Ala Gly Trp Ile Leu Thr Asp Ala Asn
100 105 110Pro Glu Phe Val Val Leu Gly Glu Thr Arg Thr Tyr Ser Phe Glu Ala
115 120 125Ile Thr Thr Ala Ile Asn Leu Ile Leu Gly Gly Ala Arg Phe Ile Cys
130 135 140Thr Asn Pro Asp Val Thr Gly Pro Ser Pro Ser Gly Ile Leu Pro Ala145 150 155 160Thr Gly Ser Val Ala Ala Leu Ile Thr Ala Ala Thr Gly Ala Glu Pro
165 170 175Tyr Tyr Ile Gly Lys Pro Asn Pro Val Met Met Arg Ser Ala Leu Asn
180 185 190Thr Ile Gly Ala His Ser Glu His Thr Val Met Ile Gly Asp Arg Met
195 200 205Asp Thr Asp Val Lys Ser Gly Leu Glu Ala Gly Leu Ser Thr Val Leu
210 215 220Val Arg Ser Gly Ile Ser Asp Asp Ala Glu Ile Arg Arg Tyr Pro Phe225 230 235 240Arg Pro Thr His Val Ile Asn Ser Ile Ala Asp Leu Ala Asp Cys Trp
245 250 255Asp Asp Pro Phe Gly Asp Gly Ala Phe His Val Pro Asp Glu Gln Gln
260 265 270Phe Thr Asp
275<210>163<211>948<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(925)<223>FRXA01468<400>163tgccaaggat ttgaccaccg tgcaggattt gattgacttt attaacacca ataaggctga 60ttagcgggaa aatttcgccc aaaacaggga caatggtgtt atg aca gtg aac att 115
Met Thr Val Asn Ile
1 5tca tat ctg acc gac atg gac ggc gtc ctc atc aaa gag ggc gag ata 163Ser Tyr Leu Thr Asp Met Asp Gly Val Leu Ile Lys Glu Gly Glu Ile
10 15 20att ccg ggt gca gat cgt ttt ctt cag tct ctc acc gat aac aat gtg 211Ile Pro Gly Ala Asp Arg Phe Leu Gln Ser Leu Thr Asp Asn Asn Val
25 30 35gag ttt atg gtt ttg acc aac aac tcc att ttc acc ccg agg gat ctt 259Glu Phe Met Val Leu Thr Asn Asn Ser Ile Phe Thr Pro Arg Asp Leu
40 45 50tct gca cgt ctt aag act tcc ggt ttg gat atc ccg ccg gag cgt att 307Ser Ala Arg Leu Lys Thr Ser Gly Leu Asp Ile Pro Pro Glu Arg Ile
55 60 65tgg act tct gca acc gcc act gct cac ttc ctg aaa tcc cag gtc aag 355Trp Thr Ser Ala Thr Ala Thr Ala His Phe Leu Lys Ser Gln Val Lys70 75 80 85gag ggc aca gcc tat gtt gtt ggc gag tcc ggt ctg acc act gcg ttg 403Glu Gly Thr Ala Tyr Val Val Gly Glu Ser Gly Leu Thr Thr Ala Leu
90 95 100cat acc gcg ggt tgg att ttg acg gat gca aat cct gag ttt gtt gtc 451His Thr Ala Gly Trp Ile Leu Thr Asp Ala Asn Pro Glu Phe Val Val
105 110 115ctg ggc gaa acc cgc aca tat tcc ttc gag gca atc act act gcg ata 499Leu Gly Glu Thr Arg Thr Tyr Ser Phe Glu Ala Ile Thr Thr Ala Ile
120 125 130aat ctg att ttg ggt ggc gct cgc ttt att tgc acc aac ccg gat gtc 547Asn Leu Ile Leu Gly Gly Ala Arg Phe Ile Cys Thr Asn Pro Asp Val
135 140 145act gga cct tca cca agt ggc att ttg cct gct act ggc tct gtc gcc 595Thr Gly Pro Ser Pro Ser Gly Ile Leu Pro Ala Thr Gly Ser Val Ala150 155 160 165gca ctt att acc gca gct act ggc gct gag cct tat tac atc ggc aag 643Ala Leu Ile Thr Ala Ala Thr Gly Ala Glu Pro Tyr Tyr Ile Gly Lys
170 175 180cca aac cct gtg atg atg cgc agt gcg ctg aac acc atc ggg gcg cat 691Pro Asn Pro Val Met Met Arg Ser Ala Leu Asn Thr Ile Gly Ala His
185 190 195tcc gag cac act gtc atg atc ggc gac cgc atg gac acc gac gtg aaa 739Ser Glu His Thr Val Met Ile Gly Asp Arg Met Asp Thr Asp Val Lys
200 205 210tct ggt ttg gaa gcc ggc ctg agc acc gtg ctg gtt cga agc gga att 787Ser Gly Leu Glu Ala Gly Leu Ser Thr Val Leu Val Arg Ser Gly Ile
215 220 225tcc gac gac gcc gag atc cgc cgc tac ccc ttc cgc cca act cac gtg 835Ser Asp Asp Ala Glu Ile Arg Arg Tyr Pro Phe Arg Pro Thr His Val230 235 240 245atc aat tcc atc gcc gat ctt gcc gat tgc tgg gac gat cct ttc ggt 883Ile Asn Ser Ile Ala Asp Leu Ala Asp Cys Trp Asp Asp Pro Phe Gly
250 255 260gac ggt gca ttt cac gta cca gat gag cag cag ttc act gac 925Asp Gly Ala Phe His Val Pro Asp Glu Gln Gln Phe Thr Asp
265 270 275tagtattctg taggtcatgg cat 948<210>164<211>275<212>PRT<213>谷氨酸棒杆菌<400>164Met Thr Val Asn Ile Ser Tyr Leu Thr Asp Met Asp Gly Val Leu Ile1 5 10 15Lys Glu Gly Glu Ile Ile Pro Gly Ala Asp Arg Phe Leu Gln Ser Leu
20 25 30Thr Asp Asn Asn Val Glu Phe Met Val Leu Thr Asn Asn Ser Ile Phe
35 40 45Thr Pro Arg Asp Leu Ser Ala Arg Leu Lys Thr Ser Gly Leu Asp Ile
50 55 60Pro Pro Glu Arg Ile Trp Thr Ser Ala Thr Ala Thr Ala His Phe Leu65 70 75 80Lys Ser Gln Val Lys Glu Gly Thr Ala Tyr Val Val Gly Glu Ser Gly
85 90 95Leu Thr Thr Ala Leu His Thr Ala Gly Trp Ile Leu Thr Asp Ala Asn
100 105 110Pro Glu Phe Val Val Leu Gly Glu Thr Arg Thr Tyr Ser Phe Glu Ala
115 120 125Ile Thr Thr Ala Ile Asn Leu Ile Leu Gly Gly Ala Arg Phe Ile Cys
130 135 140Thr Asn Pro Asp Val Thr Gly Pro Ser Pro Ser Gly Ile Leu Pro Ala145 150 155 160Thr Gly Ser Val Ala Ala Leu Ile Thr Ala Ala Thr Gly Ala Glu Pro
165 170 175Tyr Tyr Ile Gly Lys Pro Asn Pro Val Met Met Arg Ser Ala Leu Asn
180 185 190Thr Ile Gly Ala His Ser Glu His Thr Val Met Ile Gly Asp Arg Met
195 200 205Asp Thr Asp Val Lys Ser Gly Leu Glu Ala Gly Leu Ser Thr Val Leu
210 215 220Val Arg Ser Gly Ile Ser Asp Asp Ala Glu Ile Arg Arg Tyr Pro Phe225 230 235 240Arg Pro Thr His Val Ile Asn Ser Ile Ala Asp Leu Ala Asp Cys Trp
245 250 255Asp Asp Pro Phe Gly Asp Gly Ala Phe His Val Pro Asp Glu Gln Gln
260 265 270Phe Thr Asp
275<210>165<211>1128<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1105)<223>RXA00794<400>165gcgggttgat acagcccaag cgccgataca tttataatgc gcctagatac gtgcaaccca 60cgtaaccagg tcagatcaag tgccccagga ggcccttcag atg aac cta aag aac 115
Met Asn Leu Lys Asn
1 5ccc gaa acg cca gac cgt aac ctt gct atg gag ctg gtg cga gtt acg 163Pro Glu Thr Pro Asp Arg Asn Leu Ala Met Glu Leu Val Arg Val Thr
10 15 20gaa gca gct gca ctg gct tct gga cgt tgg gtt gga cgt ggc atg aag 211Glu Ala Ala Ala Leu Ala Ser Gly Arg Trp Val Gly Arg Gly Met Lys
25 30 35aat gaa ggc gac ggt gcc gct gtt gac gcc atg cgc cag ctc atc aac 259Asn Glu Gly Asp Gly Ala Ala Val Asp Ala Met Arg Gln Leu Ile Asn
40 45 50tca gtg acc atg aag ggc gtc gtt gtt atc ggc gag ggc gaa aaa gac 307Ser Val Thr Met Lys Gly Val Val Val Ile Gly Glu Gly Glu Lys Asp
55 60 65gaa gct cca atg ctg tac aac ggc gaa gag gtc gga acc ggc ttt gga 355Glu Ala Pro Met Leu Tyr Asn Gly Glu Glu Val Gly Thr Gly Phe Gly70 75 80 85cct gag gtt gat atc gca gtt gac cca gtt gac ggc acc acc ctg atg 403Pro Glu Val Asp Ile Ala Val Asp Pro Val Asp Gly Thr Thr Leu Met
90 95 100gct gag ggt cgc ccc aac gca att tcc att ctc gca gct gca gag cgt 451Ala Glu Gly Arg Pro Asn Ala Ile Ser Ile Leu Ala Ala Ala Glu Arg
105 110 115ggc acc atg tac gat cca tcc tcc gtc ttc tac atg aag aag atc gcc 499Gly Thr Met Tyr Asp Pro Ser Ser Val Phe Tyr Met Lys Lys Ile Ala
120 125 130gtg gga cct gag gcc gca ggc aag atc gac atc gaa gct cca gtt gcc 547Val Gly Pro Glu Ala Ala Gly Lys Ile Asp Ile Glu Ala Pro Val Ala
135 140 145cac aac atc aac gcg gtg gca aag tcc aag gga atc aac cct tcc gac 595His Asn Ile Asn Ala Val Ala Lys Ser Lys Gly Ile Asn Pro Ser Asp150 155 160 165gtc acc gtt gtc gtg ctt gac cgt cct cgc cac atc gaa ctg atc gca 643Val Thr Val Val Val Leu Asp Arg Pro Arg His Ile Glu Leu Ile Ala
170 175 180gac att cgt cgt gca ggc gca aag gtt cgt ctc atc tcc gac ggc gac 691Asp Ile Arg Arg Ala Gly Ala Lys Val Arg Leu Ile Ser Asp Gly Asp
185 190 195gtt gca ggt gca gtt gca gca gct cag gat tcc aac tcc gtg gac atc 739Val Ala Gly Ala Val Ala Ala Ala Gln Asp Ser Asn Ser Val Asp Ile
200 205 210atg atg ggc acc ggc gga acc cca gaa ggc atc atc act gcg tgc gcc 787Met Met Gly Thr Gly Gly Thr Pro Glu Gly Ile Ile Thr Ala Cys Ala
215 220 225atg aag tgc atg ggt ggc gaa atc cag ggc atc ctg gcc cca atg aac 835Met Lys Cys Met Gly Gly Glu Ile Gln Gly Ile Leu Ala Pro Met Asn230 235 240 245gat ttc gag cgc cag aag gca cac gac gct ggt ctg gtt ctt gat cag 883Asp Phe Glu Arg Gln Lys Ala His Asp Ala Gly Leu Val Leu Asp Gln
250 255 260gtt ctg cac acc aac gat ctg gtg agc tcc gac aac tgc tac ttc gtg 931Val Leu His Thr Asn Asp Leu Val Ser Ser Asp Asn Cys Tyr Phe Val
265 270 275gca acc ggt gtg acc aac ggt gac atg ctc cgt ggc gtt tcc tac cgc 979Ala Thr Gly Val Thr Asn Gly Asp Met Leu Arg Gly Val Ser Tyr Arg
280 285 290gca aac ggc gca acc acc cgt tcc ctg gtt atg cgc gca aag tca ggc 1027Ala Asn Gly Ala Thr Thr Arg Ser Leu Val Met Arg Ala Lys Ser Gly
295 300 305acc atc cgc cac atc gag tct gtc cac cag ctg tcc aag ctg cag gaa 1075Thr Ile Arg His Ile Glu Ser Val His Gln Leu Ser Lys Leu Gln Glu310 315 320 325tac tcc gtg gtt gac tac acc acc gcg acc taagagctct tagttcgaaa 1125Tyr Ser Val Val Asp Tyr Thr Thr Ala Thr
330 335aac 1128<210>166<211>335<212>PRT<213>谷氨酸棒杆菌<400>166Met Asn Leu Lys Asn Pro Glu Thr Pro Asp Arg Asn Leu Ala Met Glu1 5 10 15Leu Val Arg Val Thr Glu Ala Ala Ala Leu Ala Ser Gly Arg Trp Val
20 25 30Gly Arg Gly Met Lys Asn Glu Gly Asp Gly Ala Ala Val Asp Ala Met
35 40 45Arg Gln Leu Ile Asn Ser Val Thr Met Lys Gly Val Val Val Ile Gly
50 55 60Glu Gly Glu Lys Asp Glu Ala Pro Met Leu Tyr Asn Gly Glu Glu Val65 70 75 80Gly Thr Gly Phe Gly Pro Glu Val Asp Ile Ala Val Asp Pro Val Asp
85 90 95Gly Thr Thr Leu Met Ala Glu Gly Arg Pro Asn Ala Ile Ser Ile Leu
100 105 110Ala Ala Ala Glu Arg Gly Thr Met Tyr Asp Pro Ser Ser Val Phe Tyr
115 120 125Met Lys Lys Ile Ala Val Gly Pro Glu Ala Ala Gly Lys Ile Asp Ile
130 135 140Glu Ala Pro Val Ala His Asn Ile Asn Ala Val Ala Lys Ser Lys Gly145 150 155 160Ile Asn Pro Ser Asp Val Thr Val Val Val Leu Asp Arg Pro Arg His
165 170 175Ile Glu Leu Ile Ala Asp Ile Arg Arg Ala Gly Ala Lys Val Arg Leu
180 185 190Ile Ser Asp Gly Asp Val Ala Gly Ala Val Ala Ala Ala Gln Asp Ser
195 200 205Asn Ser Val Asp Ile Met Met Gly Thr Gly Gly Thr Pro Glu Gly Ile
210 215 220Ile Thr Ala Cys Ala Met Lys Cys Met Gly Gly Glu Ile Gln Gly Ile225 230 235 240Leu Ala Pro Met Asn Asp Phe Glu Arg Gln Lys Ala His Asp Ala Gly
245 250 255Leu Val Leu Asp Gln Val Leu His Thr Asn Asp Leu Val Ser Ser Asp
260 265 270Asn Cys Tyr Phe Val Ala Thr Gly Val Thr Asn Gly Asp Met Leu Arg
275 280 285Gly Val Ser Tyr Arg Ala Asn Gly Ala Thr Thr Arg Ser Leu Val Met
290 295 300Arg Ala Lys Ser Gly Thr Ile Arg His Ile Glu Ser Val His Gln Leu305 310 315 320Ser Lys Leu Gln Glu Tyr Ser Val Val Asp Tyr Thr Thr Ala Thr
325 330 335<210>167<211>1035<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1012)<223>RXN02920<400>167tgcatgcaga ttatctgtcc aactacgcca gccgcgcgta aagcgcgggc ctgctggtgg 60cgggtggcgt cgaaaagcat ttttaaagga gtttaagacg atg aag ttt gtt atg 115
Met Lys Phe Val Met
1 5tat ccg cat ttg tgg gag tcc acg acc gct gtc att gag ggt ggc gga 163Tyr Pro His Leu Trp Glu Ser Thr Thr Ala Val Ile Glu Gly Gly Gly
10 15 20cat gag cgg gtt gag gat att aaa gat gca gac ttc att ttc ttt aat 211His Glu Arg Val Glu Asp Ile Lys Asp Ala Asp Phe Ile Phe Phe Asn
25 30 35ggt tca gcg ccg gag ttc ccg gat ttg ccg gag aac atc aag ttc gtg 259Gly Ser Ala Pro Glu Phe Pro Asp Leu Pro Glu Asn Ile Lys Phe Val
40 45 50cag gcc tcc atg gcg ggt att gat gcg ctg gtc aag cgt ggt gtc gtc 307Gln Ala Ser Met Ala Gly Ile Asp Ala Leu Val Lys Arg Gly Val Val
55 60 65aat gag aag gca cgt tgg gca aac gcg gct ggc ctg tac gct gac acc 355Asn Glu Lys Ala Arg Trp Ala Asn Ala Ala Gly Leu Tyr Ala Asp Thr70 75 80 85gtt gct gag tcc acc att ggt tta att ctg gcg cag atg cac atg cat 403Val Ala Glu Ser Thr Ile Gly Leu Ile Leu Ala Gln Met His Met His
90 95 100gcg acg act cgt ttg gct aag tcg tgg agc gtg cgg cct gag gtg gaa 451Ala Thr Thr Arg Leu Ala Lys Ser Trp Ser Val Arg Pro Glu Val Glu
105 110 115aac aac aag tca tgg ctg cat gac aat aaa act gtc gct att ttg ggc 499Asn Asn Lys Ser Trp Leu His Asp Asn Lys Thr Val Ala Ile Leu Gly
120 125 130gcc ggt ggc att ggc gtg cgt ctg ctg gaa atg ctc aag ccg ttc aac 547Ala Gly Gly Ile Gly Val Arg Leu Leu Glu Met Leu Lys Pro Phe Asn
135 140 145gtg aag acc att gcg gtt aat aac tct ggt cgt ccg gtg gaa ggt gca 595Val Lys Thr Ile Ala Val Asn Asn Ser Gly Arg Pro Val Glu Gly Ala150 155 160 165gat gaa acc ttc gcc atg gat aag gct gag cac gtg tgg gct gag gct 643Asp Glu Thr Phe Ala Met Asp Lys Ala Glu His Val Trp Ala Glu Ala
170 175 180gat gtg ttt gtg ctc atc ctg ccg ctg act gat gcc act tat cag atc 691Asp Val Phe Val Leu Ile Leu Pro Leu Thr Asp Ala Thr Tyr Gln Ile
185 190 195gtc aat gca gaa act ttg ggc aag atg aag cct tct gcc gtg gtg gtc 739Val Asn Ala Glu Thr Leu Gly Lys Met Lys Pro Ser Ala Val Val Val
200 205 210aat gtg ggg cgt ggc ccg ctg atc aac acc gat gat ctg gtg gat gca 787Asn Val Gly Arg Gly Pro Leu Ile Asn Thr Asp Asp Leu Val Asp Ala
215 220 225ttg aac aac ggc acc att gcg ggt gct gcg ctg gac gtt acc gat cct 835Leu Asn Asn Gly Thr Ile Ala Gly Ala Ala Leu Asp Val Thr Asp Pro230 235 240 245gag cca ctt cct gac agc cac ccg ctg tgg gag atg gac aat gtg gtt 883Glu Pro Leu Pro Asp Ser His Pro Leu Trp Glu Met Asp Asn Val Val
250 255 260atc act cct cat act gca aac acg aat gag agg att cgt gct ttg acc 931Ile Thr Pro His Thr Ala Asn Thr Asn Glu Arg Ile Arg Ala Leu Thr
265 270 275ggc gaa ctc acc ttg cgc aac att gag ttg ttt gag gca ggc gag cag 979Gly Glu Leu Thr Leu Arg Asn Ile Glu Leu Phe Glu Ala Gly Glu Gln
280 285 290atg gcc acc gag gtc gat gtg gtg gct ggc tac taggcctttt atggtgtgat 1032Met Ala Thr Glu Val Asp Val Val Ala Gly Tyr
295 300ccg 1035<210>168<211>304<212>PRT<213>谷氨酸棒杆菌<400>168Met Lys Phe Val Met Tyr Pro His Leu Trp Glu Ser Thr Thr Ala Val1 5 10 15Ile Glu Gly Gly Gly His Glu Arg Val Glu Asp Ile Lys Asp Ala Asp
20 25 30Phe Ile Phe Phe Asn Gly Ser Ala Pro Glu Phe Pro Asp Leu Pro Glu
35 40 45Asn Ile Lys Phe Val Gln Ala Ser Met Ala Gly Ile Asp Ala Leu Val
50 55 60Lys Arg Gly Val Val Asn Glu Lys Ala Arg Trp Ala Asn Ala Ala Gly65 70 75 80Leu Tyr Ala Asp Thr Val Ala Glu Sel Thr Ile Gly Leu Ile Leu Ala
85 90 95Gln Met His Met His Ala Thr Thr Arg Leu Ala Lys Ser Trp Sel Val
100 105 110Arg Pro Glu Val Glu Asn Asn Lys Ser Trp Leu His Asp Asn Lys Thr
115 120 125Val Ala Ile Leu Gly Ala Gly Gly Ile Gly Val Arg Leu Leu Glu Met
130 135 140Leu Lys Pro Phe Asn Val Lys Thr Ile Ala Val Asn Asn Ser Gly Arg145 150 155 160Pro Val Glu Gly Ala Asp Glu Thr Phe Ala Met Asp Lys Ala Glu His
165 170 175Val Trp Ala Glu Ala Asp Val Phe Val Leu Ile Leu Pro Leu Thr Asp
180 185 190Ala Thr Tyr Gln Ile Val Asn Ala Glu Thr Leu Gly Lys Met Lys Pro
195 200 205Ser Ala Val Val Val Asn Val Gly Arg Gly Pro Leu Ile Asn Thr Asp
210 215 220Asp Leu Val Asp Ala Leu Asn Asn Gly Thr Ile Ala Gly Ala Ala Leu225 230 235 240Asp Val Thr Asp Pro Glu Pro Leu Pro Asp Ser His Pro Leu Trp Glu
245 250 255Met Asp Asn Val Val Ile Thr Pro His Thr Ala Asn Thr Asn Glu Arg
260 265 270Ile Arg Ala Leu Thr Gly Glu Leu Thr Leu Arg Asn Ile Glu Leu Phe
275 280 285Glu Ala Gly Glu Gln Met Ala Thr Glu Val Asp Val Val Ala Gly Tyr
290 295 300<210>169<211>779<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(52)..(756)<223>FRXA02379<400>169tgcaggcctc catggcgggt attgatgcgc tggtcaagcg tggtgtcgtc a atg aga 57
Met Arg
1aag cac cgt tgg gca aac gcg gct ggc ctg tac gct gac acc gtt gct 105Lys His Arg Trp Ala Asn Ala Ala Gly Leu Tyr Ala Asp Thr Val Ala
5 10 15gag tcc acc att ggt tta att ctg gcg cag atg cac atg cat gcg acg 153Glu Ser Thr Ile Gly Leu Ile Leu Ala Gln Met His Met His Ala Thr
20 25 30act cgt ttg gct aag tcg tgg agc gtg cgg cct gag gtg gaa aac aac 201Thr Arg Leu Ala Lys Ser Trp Ser Val Arg Pro Glu Val Glu Asn Asn35 40 45 50aag tca tgg ctg cat gac aat aaa act gtc gct att ttg ggc gcc ggt 249Lys Ser Trp Leu His Asp Asn Lys Thr Val Ala Ile Leu Gly Ala Gly
55 60 65ggc att ggc gtg cgt ctg ctg gaa atg ctc aag ccg ttc aac gtg aag 297Gly Ile Gly Val Arg Leu Leu Glu Met Leu Lys Pro Phe Asn Val Lys
70 75 80acc att gcg gtt aat aac tct ggt cgt ccg gtg gaa ggt gca gat gaa 345Thr Ile Ala Val Asn Asn Ser Gly Arg Pro Val Glu Gly Ala Asp Glu
85 90 95acc ttc gcc atg gat aag gct gag cac gtg tgg gct gag gct gat gtg 393Thr Phe Ala Met Asp Lys Ala Glu His Val Trp Ala Glu Ala Asp Val
100 105 110ttt gtg ctc atc ctg ccg ctg act gat gcc act tat cag atc gtc aat 441Phe Val Leu Ile Leu Pro Leu Thr Asp Ala Thr Tyr Gln Ile Val Asn115 120 125 130gca gaa act ttg ggc aag atg aag cct tct gcc gtg gtg gtc aat gtg 489Ala Glu Thr Leu Gly Lys Met Lys Pro Ser Ala Val Val Val Asn Val
135 140 145ggg cgt ggc ccg ctg atc aac acc gat gat ctg gtg gat gca ttg aac 537Gly Arg Gly Pro Leu Ile Asn Thr Asp Asp Leu Val Asp Ala Leu Asn
150 155 160aac ggc acc att gcg ggt gct gcg ctg gac gtt acc gat cct gag cca 585Asn Gly Thr Ile Ala Gly Ala Ala Leu Asp Val Thr Asp Pro Glu Pro
165 170 175ctt cct gac agc cac ccg ctg tgg gag atg gac aat gtg gtt atc act 633Leu Pro Asp Ser His Pro Leu Trp Glu Met Asp Asn Val Val Ile Thr
180 185 190cct cat act gca aac acg aat gag agg att cgt gct ttg acc ggc gaa 681Pro His Thr Ala Asn Thr Asn Glu Arg Ile Arg Ala Leu Thr Gly Glu195 200 205 210ctc acc ttg cgc aac att gag ttg ttt gag gca ggc gag cag atg gcc 729Leu Thr Leu Arg Asn Ile Glu Leu Phe Glu Ala Gly Glu Gln Met Ala
215 220 225acc gag gtc gat gtg gtg gct ggc tac taggcctttt atggtgtgat ccg 779Thr Glu Val Asp Val Val Ala Gly Tyr
230 235<210>170<211>235<212>PRT<213>谷氨酸棒杆菌<400>170Met Arg Lys His Arg Trp Ala Asn Ala Ala Gly Leu Tyr Ala Asp Thr1 5 10 15Val Ala Glu Ser Thr Ile Gly Leu Ile Leu Ala Gln Met His Met His
20 25 30Ala Thr Thr Arg Leu Ala Lys Ser Trp Ser Val Arg Pro Glu Val Glu
35 40 45Asn Asn Lys Ser Trp Leu His Asp Asn Lys Thr Val Ala Ile Leu Gly
50 55 60Ala Gly Gly Ile Gly Val Arg Leu Leu Glu Met Leu Lys Pro Phe Asn65 70 75 80Val Lys Thr Ile Ala Val Asn Asn Ser Gly Arg Pro Val Glu Gly Ala
85 90 95Asp Glu Thr Phe Ala Met Asp Lys Ala Glu His Val Trp Ala Glu Ala
100 105 110Asp Val Phe Val Leu Ile Leu Pro Leu Thr Asp Ala Thr Tyr Gln Ile
115 120 125Val Asn Ala Glu Thr Leu Gly Lys Met Lys Pro Ser Ala Val Val Val
130 135 140Asn Val Gly Arg Gly Pro Leu Ile Asn Thr Asp Asp Leu Val Asp Ala145 150 155 160Leu Asn Asn Gly Thr Ile Ala Gly Ala Ala Leu Asp Val Thr Asp Pro
165 170 175Glu Pro Leu Pro Asp Ser His Pro Leu Trp Glu Met Asp Asn Val Val
180 185 190Ile Thr Pro His Thr Ala Asn Thr Asn Glu Arg Ile Arg Ala Leu Thr
195 200 205Gly Glu Leu Thr Leu Arg Asn Ile Glu Leu Phe Glu Ala Gly Glu Gln
210 215 220Met Ala Thr Glu Val Asp Val Val Ala Gly Tyr225 230 235<210>171<211>792<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(769)<223>RXN02688<400>171gtgcggaaga cagcacgccc caaaccgacc aactagctaa gctacacaag gcggacgaat 60gggttcgcgc agcaagcgaa ggaaggaaac ttaactagcc atg gcc ggc cgg att 115
Met Ala Gly Arg Ile
1 5att ttg cta cga cac ggg cag act cac aac aac gtc aaa cac ctc ctg 163Ile Leu Leu Arg His Gly Gln Thr His Asn Asn Val Lys His Leu Leu
10 15 20gac acc cgc cca cca gga gct gaa ctc acc gac ctg ggc cgt aaa caa 211Asp Thr Arg Pro Pro Gly Ala Glu Leu Thr Asp Leu Gly Arg Lys Gln
25 30 35gcc ctt gaa gtt ggc cac gaa cta gcc acc tac tcc ggt gag cgc ctc 259Ala Leu Glu Val Gly His Glu Leu Ala Thr Tyr Ser Gly Glu Arg Leu
40 45 50gcc cat gtg tac agc tcc atc gtg ttg cgc gcc caa caa acc gcc gtg 307Ala His Val Tyr Ser Ser Ile Val Leu Arg Ala Gln Gln Thr Ala Val
55 60 65ctt gcc acc tct acc ttt gaa aaa gct cgc gac atg cag tcc ggt gcg 355Leu Ala Thr Ser Thr Phe Glu Lys Ala Arg Asp Met Gln Ser Gly Ala70 75 80 85att cca ctc gac gtt gtg gaa ggc att cag gaa atc aac gtc ggc gac 403Ile Pro Leu Asp Val Val Glu Gly Ile Gln Glu Ile Asn Val Gly Asp
90 95 100ttt gaa atg cgc ggc gat gaa gaa gcc cac atg aat tac tcc cgc gca 451Phe Glu Met Arg Gly Asp Glu Glu Ala His Met Asn Tyr Ser Arg Ala
105 110 115crc aac ggc tgg crt cac ggg gat cct gcc gct ggt crt ccc ggc ggt 499Leu Asn Gly Trp Leu His Gly Asp Pro Ala Ala Gly Leu Pro Gly Gly
120 125 130gag acc tac aaa gac gtg ctg aac cgc tac cag ccg act ctt gat cga 547Glu Thr Tyr Lys Asp Val Leu Asn Arg Tyr Gln Pro Thr Leu Asp Arg
135 140 145atc atg gac agc cac gac ctt gac gac gac cgc gac gtt gcc gtt gtc 595Ile Met Asp Ser His Asp Leu Asp Asp Asp Arg Asp Val Ala Val Val150 155 160 165agc cac ggc gcc gtc atc cgc atc gtg gca aca cac gca act ggt gtg 643Ser His Gly Ala Val Ile Arg Ile Val Ala Thr His Ala Thr Gly Val
170 175 180gat ccc aac ttt gcg ttc aac acc tac ctg ggc aac tgc cgc ttc gtg 691Asp Pro Asn Phe Ala Phe Asn Thr Tyr Leu Gly Asn Cys Arg Phe Val
185 190 195gtg ctg gag cca aac ggt aag aaa ttc agc caa tgg gat gtt gtg cgc 739Val Leu Glu Pro Asn Gly Lys Lys Phe Ser Gln Trp Asp Val Val Arg
200 205 210tgg act gac agc cca ctg cca tgg cag gag taattgagac caaaggctcg 789Trp Thr Asp Ser Pro Leu Pro Trp Gln Glu
215 220gat 792<210>172<211>223<212>PRT<213>谷氨酸棒杆菌<400>172Met Ala Gly Arg Ile Ile Leu Leu Arg His Gly Gln Thr His Asn Asn1 5 10 15Val Lys His Leu Leu Asp Thr Arg Pro Pro Gly Ala Glu Leu Thr Asp
20 25 30Leu Gly Arg Lys Gln Ala Leu Glu Val Gly His Glu Leu Ala Thr Tyr
35 40 45Ser Gly Glu Arg Leu Ala His Val Tyr Ser Ser Ile Val Leu Arg Ala
50 55 60Gln Gln Thr Ala Val Leu Ala Thr Ser Thr Phe Glu Lys Ala Arg Asp65 70 75 80Met Gln Ser Gly Ala Ile Pro Leu Asp Val Val Glu Gly Ile Gln Glu
85 90 95Ile Asn Val Gly Asp Phe Glu Met Arg Gly Asp Glu Glu Ala His Met
100 105 110Asn Tyr Ser Arg Ala Leu Asn Gly Trp Leu His Gly Asp Pro Ala Ala
115 120 125Gly Leu Pro Gly Gly Glu Thr Tyr Lys Asp Val Leu Asn Arg Tyr Gln
130 135 140Pro Thr Leu Asp Arg Ile Met Asp Ser His Asp Leu Asp Asp Asp Arg145 150 155 160Asp Val Ala Val Val Ser His Gly Ala Val Ile Arg Ile Val Ala Thr
165 170 175His Ala Thr Gly Val Asp Pro Asn Phe Ala Phe Asn Thr Tyr Leu Gly
180 185 190Asn Cys Arg Phe Val Val Leu Glu Pro Asn Gly Lys Lys Phe Ser Gln
195 200 205Trp Asp Val Val Arg Trp Thr Asp Ser Pro Leu Pro Trp Gln Glu
210 215 220<210>173<211>336<212>DNA<213>谷氨酸棒杆菌<220><22l>CDS<222>(101)..(313)<223>RXN03087<400>173gttgccgcca gccgttccag ggcgcttgag ctggtcagcg acatcgcaat gatcaaccag 60gaatacctgg aaaagagctg atattgatag ggtttaagtc atg aag atc tac gca 115
Met Lys Ile Tyr Ala
1 5cct ttt gct gga atc gtc cac tat ttt gtc gat gaa ggc gat ccc gtg 163Pro Phe Ala Gly Ile Val His Tyr Phe Val Asp Glu Gly Asp Pro Val
10 15 20gaa acc ggc atg caa ctg gga acg gta gaa acc atc aaa ctc gag gca 211Glu Thr Gly Met Gln Leu Gly Thr Val Glu Thr Ile Lys Leu Glu Ala
25 30 35cca atc atg gca ccg gga cct ggc atc gta gct aag gtt tct ttt gat 259Pro Ile Met Ala Pro Gly Pro Gly Ile Val Ala Lys Val Ser Phe Asp
40 45 50gat ttc tcc gac gtc acc ggc ggc gat gaa ctc ctc gaa ttg gag gca 307Asp Phe Ser Asp Val Thr Gly Gly Asp Glu Leu Leu Glu Leu Glu Ala
55 60 65aag aac taatgggtca aacccgcatc att 336Lys Asn70<210>174<211>71<212>PRT<213>谷氨酸棒杆菌<400>174Met Lys Ile Tyr Ala Pro Phe Ala Gly Ile Val His Tyr Phe Val Asp1 5 10 15Glu Gly Asp Pro Val Glu Thr Gly Met Gln Leu Gly Thr Val Glu Thr
20 25 30Ile Lys Leu Glu Ala Pro Ile Met Ala Pro Gly Pro Gly Ile Val Ala
35 40 45Lys Val Ser Phe Asp Asp Phe Ser Asp Val Thr Gly Gly Asp Glu Leu
50 55 60Leu Glu Leu Glu Ala Lys Asn65 70<210>175<211>310<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(310)<223>RXN03186<400>175ttcgtgcact tcggcgtgtc acaattaggt acgaccaaga atgggaccgg gaaaccggga 60cgtataaacg aaataaaaca ttccaacagg aggtgtggaa atg gcc gat caa gca 115
Met Ala Asp Gln Ala
1 5aaa ctt ggt ggc aag ccc tcg gat gac tct aac ttc gcg atg atc cgc 163Lys Leu Gly Gly Lys Pro Ser Asp Asp Ser Asn Phe Ala Met Ile Arg
10 15 20gat ggc gtg gca tct tat ttg aac gac tca gat ccg gag gag acc aac 211Asp Gly Val Ala Ser Tyr Leu Asn Asp Ser Asp Pro Glu Glu Thr Asn
25 30 35gag tgg atg gat tca ctc gac gga tta ctc cag gag tct tct cca gaa 259Gly Trp Met Asp Ser Leu Asp Gly Leu Leu Gln Glu Ser Ser Pro Glu
40 45 50cgt gct cgt tac ctc atg ctt cgt ttg ctt gag cgt gca tct gca aag 307Arg Ala Arg Tyr Leu Met Leu Arg Leu Leu Glu Arg Ala Ser Ala Lys
55 60 65cgc 310Arg70<210>176<211>70<212>PRT<213>谷氨酸棒杆菌<400>176Met Ala Asp Gln Ala Lys Leu Gly Gly Lys Pro Ser Asp Asp Ser Asn1 5 10 15Phe Ala Met Ile Arg Asp Gly Val Ala Ser Tyr Leu Asn Asp Ser Asp
20 25 30Pro Glu Glu Thr Asn Glu Trp Met Asp Ser Leu Asp Gly Leu Leu Gln
35 40 45Glu Ser Ser Pro Glu Arg Ala Arg Tyr Leu Met Leu Arg Leu Leu Glu
50 55 60Arg Ala Ser Ala Lys Arg65 70<210>177<211>302<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(279)<223>RXN03187<400>177gtt gca gtg tct gac ttc tcc act gat ctg cca aac cag atc cgt gaa 48Val Ala Val Ser Asp Phe Ser Thr Asp Leu Pro Asn Gln Ile Arg Glu1 5 10 15tgg gtc cca ggc gac tac acc gtt ctc ggt gca gat ggc ttc ggt ttc 96Trp Val Pro Gly Asp Tyr Thr Val Leu Gly Ala Asp Gly Phe Gly Phe
20 25 30tct gat acc cgc cca gct gct cgt cgc ttc ttc aac atc gac gct gag 144Ser Asp Thr Arg Pro Ala Ala Arg Arg Phe Phe Asn Ile Asp Ala Glu
35 40 45tcc att gtt gtt gca gtg ctg aac tcc ctg gca cgc gaa ggc aag atc 192Ser Ile Val Val Ala Val Leu Asn Ser Leu Ala Arg Glu Gly Lys Ile
50 55 60gac gtc tcc gtt gct gct cag gct gct gag aag ttc aag ttg gat gat 240Asp Val Ser Val Ala Ala Gln Ala Ala Glu Lys Phe Lys Leu Asp Asp65 70 75 80cct acg agt gtt tcc gta gat cca aac gct cct gag gaa taaatcacct 289Pro Thr Ser Val Ser Val Asp Pro Asn Ala Pro Glu Glu
85 90caagggacag ata 302<210>178<211>93<212>PRT<213>谷氨酸棒杆菌<400>178Val Ala Val Ser Asp Phe Ser Thr Asp Leu Pro Asn Gln Ile Arg Glu1 5 10 15Trp Val Pro Gly Asp Tyr Thr Val Leu Gly Ala Asp Gly Phe Gly Phe
20 25 30Ser Asp Thr Arg Pro Ala Ala Arg Arg Phe Phe Asn Ile Asp Ala Glu
35 40 45Ser Ile Val Val Ala Val Leu Asn Ser Leu Ala Arg Glu Gly Lys Ile
50 55 60Asp Val Ser Val Ala Ala Gln Ala Ala Glu Lys Phe Lys Leu Asp Asp65 70 75 80Pro Thr Ser Val Ser Val Asp Pro Asn Ala Pro Glu Glu
85 90<210>179<211>1953<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1930)<223>RXN02591<400>179atgtgtccgt tgtctcacct aaagttttaa ctagttctgt atctgaaagc tacgctaggg 60ggcgagaact ctgtcgaatg acacaaaatc tggagaagta atg act act gct gca 115
Met Thr Thr Ala Ala
1 5atc agg ggc ctt cag ggc gag gcg ccg acc aag aat aag gaa ctg ctg 163Ile Arg Gly Leu Gln Gly Glu Ala Pro Thr Lys Asn Lys Glu Leu Leu
10 15 20aac tgg atc gca gac gcc gtc gag ctc ttc cag cct gag gct gtt gtg 211Asn Trp Ile Ala Asp Ala Val Glu Leu Phe Gln Pro Glu Ala Val Val
25 30 35ttc gtt gat gga tcc cag gct gag tgg gat cgc atg gcg gag gat ctt 259Phe Val Asp Gly Ser Gln Ala Glu Trp Asp Arg Met Ala Glu Asp Leu
40 45 50gtt gaa gcc ggt acc ctc atc aag ctc aac gag gaa aag cgt ccg aac 307Val Glu Ala Gly Thr Leu Ile Lys Leu Asn Glu Glu Lys Arg Pro Asn
55 60 65agc tac cta gct cgt tcc aac cca tct gac gtt gcg cgc gtt gag tcc 355Ser Tyr Leu Ala Arg Ser Asn Pro Ser Asp Val Ala Arg Val Glu Ser70 75 80 85cgc acc ttc atc tgc tcc gag aag gaa gaa gat gct ggc cca acc aac 403Arg Thr Phe Ile Cys Ser Glu Lys Glu Glu Asp Ala Gly Pro Tnr Asn
90 95 100aac tgg gct cca cca cag gca atg aag gac gaa atg tcc aag cat tac 451Asn Trp Ala Pro Pro Gln Ala Met Lys Asp Glu Met Ser Lys His Tyr
105 110 115gct ggt tcc atg aag ggg cgc acc atg tac gtc gtg cct ttc tgc atg 499Ala Gly Ser Met Lys Gly Arg Thr Met Tyr Val Val Pro Phe Cys Met
120 125 130ggt cca atc agc gat ccg gac cct aag ctt ggt gtg cag ctc act gac 547Gly Pro Ile Ser Asp Pro Asp Pro Lys Leu Gly Val Gln Leu Thr Asp
135 140 145tcc gag tac gtt gtc atg tcc atg cgc atc atg acc cgc atg ggt att 595Ser Glu Tyr Val Val Met Ser Met Arg Ile Met Thr Arg Met Gly Ile150 155 160 165gaa gcg ctg gac aag atc ggc gcg aac ggc agc ttc gtc agg tgc ctc 643Glu Ala Leu Asp Lys Ile Gly Ala Asn Gly Ser Phe Val Arg Cys Leu
170 175 180cac tcc gtt ggt gct cct ttg gag cca ggc cag gaa gac gtt gca tgg 691His Ser Val Gly Ala Pro Leu Glu Pro Gly Gln Glu Asp Val Ala Trp
185 190 195cct tgc aac gac acc aag tac atc acc cag ttc cca gag acc aag gaa 739Pro Cys Asn Asp Thr Lys Tyr Ile Thr Gln Phe Pro Glu Thr Lys Glu
200 205 210att tgg tcc tac ggt tcc ggc tac ggc gga aac gca atc ctg gca aag 787Ile Trp Ser Tyr Gly Ser Gly Tyr Gly Gly Asn Ala Ile Leu Ala Lys
215 220 225aag tgc tac gca ctg cgt atc gca tct gtc atg gct cgc gaa gaa gga 835Lys Cys Tyr Ala Leu Arg Ile Ala Ser Val Met Ala Arg Glu Glu Gly230 235 240 245tgg atg gct gag cac atg ctc atc ctg aag ctg atc aac cca gag ggc 883Trp Met Ala Glu His Met Leu Ile Leu Lys Leu Ile Asn Pro Glu Gly
250 255 260aag gcg tac cac atc gca gca gca ttc cca tct gct tgt ggc aag acc 931Lys Ala Tyr His Ile Ala Ala Ala Phe Pro Ser Ala Cys Gly Lys Thr
265 270 275aac ctc gcc atg atc act cca acc atc cca ggc tgg acc gct cag gtt 979Asn Leu Ala Met Ile Thr Pro Thr Ile Pro Gly Trp Thr Ala Gln Val
280 285 290gtt ggc gac gac atc gct tgg ctg aag ctg cgc gag gac ggc ctc tac 1027Val Gly Asp Asp Ile Ala Trp Leu Lys Leu Arg Glu Asp Gly Leu Tyr
295 300 305gca gtt aac cca gaa aat ggt ttc ttc ggt gtt gct cca ggc acc aac 1075Ala Val Asn Pro Glu Asn Gly Phe Phe Gly Val Ala Pro Gly Thr Asn310 315 320 325tac gca tcc aac cca atc gcg atg aag acc atg gaa cca ggc aac acc 1123Tyr Ala Ser Asn Pro Ile Ala Met Lys Thr Met Glu Pro Gly Asn Thr
330 335 340ctg ttc acc aac gtg gca ctc acc gac gac ggc gac atc tgg tgg gaa 1171Leu Phe Thr Asn Val Ala Leu Thr Asp Asp Gly Asp Ile Trp Trp Glu
345 350 355ggc atg gac ggc gac gcc cca gct cac ctc att gac tgg atg ggc aac 1219Gly Met Asp Gly Asp Ala Pro Ala His Leu Ile Asp Trp Met Gly Asn
360 365 370gac tgg acc cca gag tcc gac gaa aac gct gct cac cct aac tcc cgt 1267Asp Trp Thr Pro Glu Ser Asp Glu Asn Ala Ala His Pro Asn Ser Arg
375 380 385tac tgc gta gca atc gac cag tcc cca gca gca gca cct gag ttc aac 1315Tyr Cys Val Ala Ile Asp Gln Ser Pro Ala Ala Ala Pro Glu Phe Asn390 395 400 405gac tgg gaa ggc gtc aag atc gac gca atc ctc ttc ggt gga cgt cgc 1363Asp Trp Glu Gly Val Lys Ile Asp Ala Ile Leu Phe Gly Gly Arg Arg
410 415 420gca gac acc gtc cca ctg gtt acc cag acc tac gac tgg gag cac ggc 1411Ala Asp Thr Val Pro Leu Val Thr Gln Thr Tyr Asp Trp Glu His Gly
425 430 435acc atg gtt ggt gca ctg ctc gca tcc ggt cag acc gca gct tcc gca 1459Thr Met Val Gly Ala Leu Leu Ala Ser Gly Gln Thr Ala Ala Ser Ala
440 445 450gaa gca aag gtc ggc aca ctc cgc cac gac cca atg gca atg ctc cca 1507Glu Ala Lys Val Gly Thr Leu Arg His Asp Pro Met Ala Met Leu Pro
455 460 465ttc att ggc tac aac gct ggt gaa tac ctg cag aac tgg att gac atg 1555Phe Ile Gly Tyr Asn Ala Gly Glu Tyr Leu Gln Asn Trp Ile Asp Met470 475 480 485ggt aac aag ggt ggc gac aag atg cca tcc atc ttc ctg gtc aac tgg 1603Gly Asn Lys Gly Gly Asp Lys Met Pro Ser Ile Phe Leu Val Asn Trp
490 495 500ttc cgc cgt ggc gaa gat gga cgc ttc ctg tgg cct ggc ttc ggc gac 1651Phe Arg Arg Gly Glu Asp Gly Arg Phe Leu Trp Pro Gly Phe Gly Asp
505 510 515aac tct cgc gtt ctg aag tgg gtc atc gac cgc atc gaa ggc cac gtt 1699Asn Ser Arg Val Leu Lys Trp Val Ile Asp Arg Ile Glu Gly His Val
520 525 530ggc gca gac gag acc gtt gtt gga cac acc gct aag gcc gaa gac ctc 1747Gly Ala Asp Glu Thr Val Val Gly His Thr Ala Lys Ala Glu Asp Leu
535 540 545gac ctc gac ggc ctc gac acc cca att gag gat gtc aag gaa gca ctg 1795Asp Leu Asp Gly Leu Asp Thr Pro Ile Glu Asp Val Lys Glu Ala Leu550 555 560 565acc gct cct gca gag cag tgg gca aac gac gtt gaa gac aac gcc gag 1843Thr Ala Pro Ala Glu Gln Trp Ala Asn Asp Val Glu Asp Asn Ala Glu
570 575 580tac ctc act ttc ctc gga cca cgt gtt cct gca gag gtt cac agc cag 1891Tyr Leu Thr Phe Leu Gly Pro Arg Val Pro Ala Glu Val His Ser Gln
585 590 595ttc gat gct ctg aag gcc cgc att tca gca gct cac gct taaagttcac 1940Phe Asp Ala Leu Lys Ala Arg Ile Ser Ala Ala His Ala
600 605 610gcttaagaac tgc 1953<210>180<211>610<212>PRT<213>谷氨酸棒杆菌<400>180Met Thr Thr Ala Ala Ile Arg Gly Leu Gln Gly Glu Ala Pro Thr Lys1 5 10 15Asn Lys Glu Leu Leu Asn Trp Ile Ala Asp Ala Val Glu Leu Phe Gln
20 25 30Pro Glu Ala Val Val Phe Val Asp Gly Ser Gln Ala Glu Trp Asp Arg
35 40 45Met Ala Glu Asp Leu Val Glu Ala Gly Thr Leu Ile Lys Leu Asn Glu
50 55 60Glu Lys Arg Pro Asn Ser Tyr Leu Ala Arg Ser Asn Pro Ser Asp Val65 70 75 80Ala Arg Val Glu Ser Arg Thr Phe Ile Cys Ser Glu Lys Glu Glu Asp
85 90 95Ala Gly Pro Thr Asn Asn Trp Ala Pro Pro Gln Ala Met Lys Asp Glu
100 105 110Met Ser Lys His Tyr Ala Gly Ser Met Lys Gly Arg Thr Met Tyr Val
115 120 125Val Pro Phe Cys Met Gly Pro Ile Ser Asp Pro Asp Pro Lys Leu Gly
130 135 140Val Gln Leu Thr Asp Ser Glu Tyr Val Val Met Ser Met Arg Ile Met145 150 155 160Thr Arg Met Gly Ile Glu Ala Leu Asp Lys Ile Gly Ala Asn Gly Ser
165 170 175Phe Val Arg Cys Leu His Ser Val Gly Ala Pro Leu Glu Pro Gly Gln
180 185 190Glu Asp Val Ala Trp Pro Cys Asn Asp Thr Lys Tyr Ile Thr Gln Phe
195 200 205Pro Glu Thr Lys Glu Ile Trp Ser Tyr Gly Ser Gly Tyr Gly Gly Asn
210 215 220Ala Ile Leu Ala Lys Lys Cys Tyr Ala Leu Arg Ile Ala Ser Val Met225 230 235 240Ala Arg Glu Glu Gly Trp Met Ala Glu His Met Leu Ile Leu Lys Leu
245 250 255Ile Asn Pro Glu Gly Lys Ala Tyr His Ile Ala Ala Ala Phe Pro Ser
260 265 270Ala Cys Gly Lys Thr Asn Leu Ala Met Ile Thr Pro Thr Ile Pro Gly
275 280 285Trp Thr Ala Gln Val Val Gly Asp Asp Ile Ala Trp Leu Lys Leu Arg
290 295 300Glu Asp Gly Leu Tyr Ala Val Asn Pro Glu Asn Gly Phe Phe Gly Val305 310 315 320Ala Pro Gly Thr Asn Tyr Ala Ser Asn Pro Ile Ala Met Lys Thr Met
325 330 335Glu Pro Gly Asn Thr Leu Phe Thr Asn Val Ala Leu Thr Asp Asp Gly
340 345 350Asp Ile Trp Trp Glu Gly Met Asp Gly Asp Ala Pro Ala His Leu Ile
355 360 365Asp Trp Met Gly Asn Asp Trp Thr Pro Glu Ser Asp Glu Asn Ala Ala
370 375 380His Pro Asn Ser Arg Tyr Cys Val Ala Ile Asp Gln Ser Pro Ala Ala385 390 395 400Ala Pro Glu Phe Asn Asp Trp Glu Gly Val Lys Ile Asp Ala Ile Leu
405 410 415Phe Gly Gly Arg Arg Ala Asp Thr Val Pro Leu Val Thr Gln Thr Tyr
420 425 430Asp Trp Glu His Gly Thr Met Val Gly Ala Leu Leu Ala Ser Gly Gln
435 440 445Thr Ala Ala Ser Ala Glu Ala Lys Val Gly Thr Leu Arg His Asp Pro
450 455 460Met Ala Met Leu Pro Phe Ile Gly Tyr Asn Ala Gly Glu Tyr Leu Gln465 470 475 480Asn Trp Ile Asp Met Gly Asn Lys Gly Gly Asp Lys Met Pro Ser Ile
485 490 495Phe Leu Val Asn Trp Phe Arg Arg Gly Glu Asp Gly Arg Phe Leu Trp
500 505 510Pro Gly Phe Gly Asp Asn Ser Arg Val Leu Lys Trp Val Ile Asp Arg
515 520 525Ile Glu Gly His Val Gly Ala Asp Glu Thr Val Val Gly His Thr Ala
530 535 540Lys Ala Glu Asp Leu Asp Leu Asp Gly Leu Asp Thr Pro Ile Glu Asp545 550 555 560Val Lys Glu Ala Leu Thr Ala Pro Ala Glu Gln Trp Ala Asn Asp Val
565 570 575Glu Asp Asn Ala Glu Tyr Leu Thr Phe Leu Gly Pro Arg Val Pro Ala
580 585 590Glu Val His Ser Gln Phe Asp Ala Leu Lys Ala Arg Ile Ser Ala Ala
595 600 605His Ala
610<210>181<211>1305<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1282)<223>RXS01260<400>181ctaaacgtgg gctgcattcc ttccaaagtc tctgatcaaa aacgctgaag ttgcccatac 60ctttacccat gagaagaaga ccttcggcat caatggcgaa gtg acc ttc aac tat 115
Val Thr Phe Asn Tyr
1 5gag gat gct cac aag cgt tcc cgt ggc gtt tcc gac aag atc gtt gga 163Glu Asp Ala His Lys Arg Ser Arg Gly Val Ser Asp Lys Ile Val Gly
10 15 20ggc gtt cat tac ttg atg aag aag aac aag atc atc gaa att cat ggt 211Gly Val His Tyr Leu Met Lys Lys Asn Lys Ile Ile Glu Ile His Gly
25 30 35ctt gga aac ttc aag gat gct aag act ctt gag gtc acc gac ggt aag 259Leu Gly Asn Phe Lys Asp Ala Lys Thr Leu Glu Val Thr Asp Gly Lys
40 45 50gat gct ggc aag acc atc acc ttt gat gac tgc atc atc gca acc ggt 307Asp Ala Gly Lys Thr Ile Thr Phe Asp Asp Cys Ile Ile Ala Thr Gly
55 60 65tcg gta gtc aac acc ctc cgt ggc gtt gac ttc tca gag aac gtt gtg 355Ser Val Val Asn Thr Leu Arg Gly Val Asp Phe Ser Glu Asn Val Val70 75 80 85tct ttt gaa gag cag att ctt aac cct gtt gcg cca aag aag atg gtc 403Ser Phe Glu Glu Gln Ile Leu Asn Pro Val Ala Pro Lys Lys Met Val
90 95 100att gtt ggt gca ggc gca att gga atg gaa ttc gcc tac gtt ctt ggt 451Ile Val Gly Ala Gly Ala Ile Gly Met Glu Phe Ala Tyr Val Leu Gly
105 110 115aac tac ggt gta gat gta acc gtc atc gag ttc atg gat cgt gtg ctt 499Asn Tyr Gly Val Asp Val Thr Val Ile Glu Phe Met Asp Arg Val Leu
120 125 130cca aat gaa gat gct gaa gtc tcc aag gtt att gca aag gcc tac aag 547Pro Asn Glu Asp Ala Glu Val Ser Lys Val Ile Ala Lys Ala Tyr Lys
135 140 145aag atg ggc gtt aag ctt ctt cct ggc cat gca acc act gct gtt cgg 595Lys Met Gly Val Lys Leu Leu Pro Gly His Ala Thr Thr Ala Val Arg150 155 160 165gac aac ggt gac ttt gtc gag gtt gat tac cag aag aag ggc tct gac 643Asp Asn Gly Asp Phe Val Glu Val Asp Tyr Gln Lys Lys Gly Ser Asp
170 175 180aag aca gag act ctt act gtt gat cga gtc atg gtt tcc gtt ggt ttc 691Lys Thr Glu Thr Leu Thr Val Asp Arg Val Met Val Ser Val Gly Phe
185 190 195cgt cca cgc gtt gag gga ttt ggt ctt gaa aac act ggc gtt aag ctc 739Arg Pro Arg Val Glu Gly Phe Gly Leu Glu Asn Thr Gly Val Lys Leu
200 205 210acc gag cgt ggc gca atc gag atc gat gat tac atg cgt acc aac gtc 787Thr Glu Arg Gly Ala Ile Glu Ile Asp Asp Tyr Met Arg Thr Asn Val
215 220 225gat ggc att tac gcc atc ggt gac gtg acc gcc aag ctt cag ctt gct 835Asp Gly Ile Tyr Ala Ile Gly Asp Val Thr Ala Lys Leu Gln Leu Ala230 235 240 245cac gtc gca gaa gca cag ggc att gtt gcc gca gag act att gct ggt 883His Val Ala Glu Ala Gln Gly Ile Val Ala Ala Glu Thr Ile Ala Gly
250 255 260gca gaa act cag act ctt ggt gat tac atg atg atg cca cgt gca acc 931Ala Glu Thr Gln Thr Leu Gly Asp Tyr Met Met Met Pro Arg Ala Thr
265 270 275ttc tgc aac cca cag gtt tct tcc ttt ggt tac acc gaa gag cag gcc 979Phe Cys Asn Pro Gln Val Ser Ser Phe Gly Tyr Thr Glu Glu Gln Ala
280 285 290aag gag aag tgg cca gat cgt gag atc aag gtt gct tcc ttc cca ttc 1027Lys Glu Lys Trp Pro Asp Arg Glu Ile Lys Val Ala Ser Phe Pro Phe
295 300 305tct gca aac ggt aaa gca gtt ggc ctg gca gaa act gat ggt ttc gca 1075Ser Ala Asn Gly Lys Ala Val Gly Leu Ala Glu Thr Asp Gly Phe Ala310 315 320 325aag atc gtt gct gat gca gaa ttc ggt gag ctg ctc ggt gca cac ctg 1123Lys Ile Val Ala Asp Ala Glu Phe Gly Glu Leu Leu Gly Ala His Leu
330 335 340gtt gga gca aat gca tca gag ctc atc aat gaa ttg gtg ctt gct cag 1171Val Gly Ala Asn Ala Ser Glu Leu Ile Asn Glu Leu Val Leu Ala Gln
345 350 355aac tgg gat ctc acc act gaa gag atc tct cgt agc gtc cat att cac 1219Asn Trp Asp Leu Thr Thr Glu Glu Ile Ser Arg Ser Val His Ile His
360 365 370cca acg cta tct gag gca gtt aag gaa gct gca cac ggt atc tct gga 1267Pro Thr Leu Ser Glu Ala Val Lys Glu Ala Ala His Gly Ile Ser Gly
375 380 385cac atg atc aac ttc tagaatccac ctcgttggcc ctg 1305His Met Ile Asn Phe390<210>182<211>394<212>PRT<213>谷氨酸棒杆菌<400>182Val Thr Phe Asn Tyr Glu Asp Ala His Lys Arg Ser Arg Gly Val Ser1 5 10 15Asp Lys Ile Val Gly Gly Val His Tyr Leu Met Lys Lys Asn Lys Ile
20 25 30Ile Glu Ile His Gly Leu Gly Asn Phe Lys Asp Ala Lys Thr Leu Glu
35 40 45Val Thr Asp Gly Lys Asp Ala Gly Lys Thr Ile Thr Phe Asp Asp Cys
50 55 60Ile Ile Ala Thr Gly Ser Val Val Asn Thr Leu Arg Gly Val Asp Phe65 70 75 80Ser Glu Asn Val Val Ser Phe Glu Glu Gln Ile Leu Asn Pro Val Ala
85 90 95Pro Lys Lys Met Val Ile Val Gly Ala Gly Ala Ile Gly Met Glu Phe
100 105 110Ala Tyr Val Leu Gly Asn Tyr Gly Val Asp Val Thr Val Ile Glu Phe
115 120 125Met Asp Arg Val Leu Pro Asn Glu Asp Ala Glu Val Ser Lys Val Ile
130 135 140Ala Lys Ala Tyr Lys Lys Met Gly Val Lys Leu Leu Pro Gly His Ala145 150 155 160Thr Thr Ala Val Arg Asp Asn Gly Asp Phe Val Glu Val Asp Tyr Gln
165 170 175Lys Lys Gly Ser Asp Lys Thr Glu Thr Leu Thr Val Asp Arg Val Met
180 185 190Val Ser Val Gly Phe Arg Pro Arg Val Glu Gly Phe Gly Leu Glu Asn
195 200 205Thr Gly Val Lys Leu Thr Glu Arg Gly Ala Ile Glu Ile Asp Asp Tyr
210 215 220Met Arg Thr Asn Val Asp Gly Ile Tyr Ala Ile Gly Asp Val Thr Ala225 230 235 240Lys Leu Gln Leu Ala His Val Ala Glu Ala Gln Gly Ile Val Ala Ala
245 250 255Glu Thr Ile Ala Gly Ala Glu Thr Gln Thr Leu Gly Asp Tyr Met Met
260 265 270Met Pro Arg Ala Thr Phe Cys Asn Pro Gln Val Ser Ser Phe Gly Tyr
275 280 285Thr Glu Glu Gln Ala Lys Glu Lys Trp Pro Asp Arg Glu Ile Lys Val
290 295 300Ala Ser Phe Pro Phe Ser Ala Asn Gly Lys Ala Val Gly Leu Ala Glu305 310 315 320Thr Asp Gly Phe Ala Lys Ile Val Ala Asp Ala Glu Phe Gly Glu Leu
325 330 335Leu Gly Ala His Leu Val Gly Ala Asn Ala Ser Glu Leu Ile Asn Glu
340 345 350Leu Val Leu Ala Gln Asn Trp Asp Leu Thr Thr Glu Glu Ile Ser Arg
355 360 365Ser Val His Ile His Pro Thr Leu Ser Glu Ala Val Lys Glu Ala Ala
370 375 380His Gly Ile Ser Gly His Met Ile Asn Phe385 390<210>183<211>294<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(271)<223>RXS01261<400>183gtgggtgttt ttcattttct tccactctaa aattaagtat ggaaaaccaa ccgcacccgg 60atgcacgaca atgacccact aaacacgtat ccttgaatgc gtg act gaa cat tat 115
Val Thr Glu His Tyr
1 5gac gta gta gta ctc gga gcc ggc ccc ggt ggc tat gtc tcc gcc atc 163Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly Tyr Val Ser Ala Ile
10 15 20cgt gca gcg cag ctt ggc aag aag gtt gct gta att gag aag cag tac 211Arg Ala Ala Gln Leu Gly Lys Lys Val Ala Val Ile Glu Lys Gln Tyr
25 30 35tgg ggt ggt gtt tgc cta aac gtg ggc tgc att cct tcc aaa gtc tct 259Trp Gly Gly Val Cys Leu Asn Val Gly Cys Ile Pro Ser Lys Val Ser
40 45 50gat caa aaa cgc tgaagttgcc cataccttta ccc 294Asp Gln Lys Arg
55<210>184<211>57<212>PRT<213>谷氨酸棒杆菌<400>184Val Thr Glu His Tyr Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly1 5 10 15Tyr Val Ser Ala Ile Arg Ala Ala Gln Leu Gly Lys Lys Val Ala Val
20 25 30Ile Glu Lys Gln Tyr TrP Gly Gly Val Cys Leu Asn Val Gly Cys Ile
35 40 45Pro Ser Lys Val Ser Asp Gln Lys Arg
50 55<210>185<211>1650<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1627)<223>RXA02640<400>185accaacgacg acgccggtgt agcagatgta ttggagtggt ggttctaata ggtggtgtta 60aaacactgct tagtggccca atacgtgcaa aaataaggcc atg aga atc tca aag 115
Met Arg Ile Ser Lys
1 5gcc aat gcg tat gtt gca gcg att gac caa ggc acc act tcc act cgg 163Ala Asn Ala Tyr Val Ala Ala Ile Asp Gln Gly Thr Thr Ser Thr Arg
10 15 20tgc atc ttc att gat gcc caa gga aaa gtg gtg tct tct gct tcc aag 211Cys Ile Phe Ile Asp Ala Gln Gly Lys Val Val Ser Ser Ala Ser Lys
25 30 35gag cac cgc caa atc ttc cca caa cag ggc tgg gta gag cac gat cct 259Glu His Arg Gln Ile Phe Pro Gln Gln Gly Trp Val Glu His Asp Pro
40 45 50gaa gaa att tgg gac aac att cga tct gtc gtc agc cag gcg atg gtc 307Glu Glu Ile Trp Asp Asn Ile Arg Ser Val Val Ser Gln Ala Met Val
55 60 65tcc att gac atc acc cca cac gag gtt gca tcg ctg gga gtc acc aac 355Ser Ile Asp Ile Thr Pro His Glu Val Ala Ser Leu Gly Val Thr Asn70 75 80 85cag cgc gaa acc acc gtg gtg tgg gac aag cac acc ggc gaa cct gtc 403Gln Arg Glu Thr Thr Val Val Trp Asp Lys His Thr Gly Glu Pro Val
90 95 100tac aac gca atc gtg tgg caa gac acc cgc acc tct gac att tgc cta 451Tyr Asn Ala Ile Val Trp Gln Asp Thr Arg Thr Ser Asp Ile Cys Leu
105 110 115gag atc gcg ggc gaa gaa ggc cag gaa aag tgg ctt gac cgc acc ggc 499Glu Ile Ala Gly Glu Glu Gly Gln Glu Lys Trp Leu Asp Arg Thr Gly
120 125 130ctg ctg atc aac tcc tac cca tcg ggg ccc aaa atc aag tgg att ctc 547Leu Leu Ile Asn Ser Tyr Pro Ser Gly Pro Lys Ile Lys Trp Ile Leu
135 140 145gac aac gtt gag gga gct cgc gaa cgc gcc gaa aag ggc gac ctt ttg 595Asp Asn Val Glu Gly Ala Arg Glu Arg Ala Glu Lys Gly Asp Leu Leu150 155 160 165ttt ggc acc atg gat acc tgg gtg ctg tgg aac ctg acc ggc ggt gtc 643Phe Gly Thr Met Asp Thr Trp Val Leu Trp Asn Leu Thr Gly Gly Val
170 175 180cgc ggc gac gac ggt gat gat gcc atc cac gtc acc gat gtc acc aac 691Arg Gly Asp Asp Gly Asp Asp Ala Ile His Val Thr Asp Val Thr Asn
185 190 195gca tcc cgc aca cta ttg atg gat ctc cgc acg caa cag tgg gat cca 739Ala Ser Arg Thr Leu Leu Met Asp Leu Arg Thr Gln Gln Trp Asp Pro
200 205 210gaa cta tgc gaa gcc cta gac att ccg atg tcc atg ctc cct gag att 787Glu Leu Cys Glu Ala Leu Asp Ile Pro Met Ser Met Leu Pro Glu Ile
215 220 225cgt ccc tcc gtc gga gaa ttc cgc tcc gtg cgc cac cgc gga acc cta 835Arg Pro Ser Val Gly Glu Phe Arg Ser Val Arg His Arg Gly Thr Leu230 235 240 245gcc gac gtc ccg att act ggc gtg ctc ggc gac cag caa gcg gcc ctt 883Ala Asp Val Pro Ile Thr Gly Val Leu Gly Asp Gln Gln Ala Ala Leu
250 255 260ttt ggt cag ggc gga ttc cac gaa ggt gct gct aaa aat acc tac ggc 931Phe Gly Gln Gly Gly Phe His Glu Gly Ala Ala Lys Asn Thr Tyr Gly
265 270 275acc ggc ctc ttc ctg ctg atg aac acc ggc acc tcg ttg aag att tcc 979Thr Gly Leu Phe Leu Leu Met Asn Thr Gly Thr Ser Leu Lys Ile Ser
280 285 290gag cac ggc ctg ctg tcc acc atc gcc tat caa cgg gaa gga tcc gct 1027Glu His Gly Leu Leu Ser Thr Ile Ala Tyr Gln Arg Glu Gly Ser Ala
295 300 305ccg gtc tac gcg ctg gaa ggt tcc gta tcc atg ggc ggt tcc ttg gtg 1075Pro Val Tyr Ala Leu Glu Gly Ser Val Ser Met Gly Gly Ser Leu Val310 315 320 325cag tgg ctg cgc gac aac cta cag cta atc ccc aac gca cca gcg att 1123Gln Trp Leu Arg Asp Asn Leu Gln Leu Ile Pro Asn Ala Pro Ala Ile
330 335 340gaa aac ctc gcc cga gaa gtc gaa gac aac ggt ggc gtt cat gtt gtc 1171Glu Asn Leu Ala Arg Glu Val Glu Asp Asn Gly Gly Val His Val Val
345 350 355cca gca ttc acc gga ctg ttc gca cca cgt tgg cgc ccc gat gct cgt 1219Pro Ala Phe Thr Gly Leu Phe Ala Pro Arg Trp Arg Pro Asp Ala Arg
360 365 370ggc gtc att aca ggc ctc acc cgt ttt gcc aac cgc aaa cac atc gcc 1267Gly Val Ile Thr Gly Leu Thr Arg Phe Ala Asn Arg Lys His Ile Ala
375 380 385cgc gca gtc ctt gaa gcc aac gcc ttc caa acc cgc gaa gtt gtg gac 1315Arg Ala Val Leu Glu Ala Asn Ala Phe Gln Thr Arg Glu Val Val Asp390 395 400 405gcc atg gcc aaa gac gca ggc aaa gcc ctc gaa tcc ctc cgc gtc gac 1363Ala Met Ala Lys Asp Ala Gly Lys Ala Leu Glu Ser Leu Arg Val Asp
410 415 420ggt gcg atg gtg gaa aat gac ctc ctc atg caa atg caa gcc gac ttc 1411Gly Ala Met Val Glu Asn Asp Leu Leu Met Gln Met Gln Ala Asp Phe
425 430 435ctc ggc atc gac gtc caa cgt ctc gag gac gta gaa acc acc gcc gtc 1459Leu Gly Ile Asp Val Gln Arg Leu Glu Asp Val Glu Thr Thr Ala Val
440 445 450ggc gtc gca ttc gct gca ggt ctc ggc tct gga ttc ttc aaa aca act 1507Gly Val Ala Phe Ala Ala Gly Leu Gly Ser Gly Phe Phe Lys Thr Thr
455 460 465gac gag atc gaa aaa ctt att gca gtg aag aaa gtc tgg aac cct gac 1555Asp Glu Ile Glu Lys Leu Ile Ala Val Lys Lys Val Trp Asn Pro Asp470 475 480 485atg agc gaa gaa gag cgc gaa cgt cgc tat gcc gaa tgg aat agg gca 1603Met Ser Glu Glu Glu Arg Glu Arg Arg Tyr Ala Glu Trp Asn Arg Ala
490 495 500gtg gag cat tct tat gac cag gcc tagctgattt gggtcggcct tta 1650Val Glu His Ser Tyr Asp Gln Ala
505<210>186<211>509<212>PRT<213>谷氨酸棒杆菌<400>186Met Arg Ile Ser Lys Ala Asn Ala Tyr Val Ala Ala Ile Asp Gln Gly1 5 10 15Thr Thr Ser Thr Arg Cys Ile Phe Ile Asp Ala Gln Gly Lys Val Val
20 25 30Ser Ser Ala Ser Lys Glu His Arg Gln Ile Phe Pro Gln Gln Gly Trp
35 40 45Val Glu His Asp Pro Glu Glu Ile Trp Asp Asn Ile Arg Ser Val Val
50 55 60Ser Gln Ala Met Val Ser Ile Asp Ile Thr Pro His Glu Val Ala Ser65 70 75 80Leu Gly Val Thr Asn Gln Arg Glu Thr Thr Val Val Trp Asp Lys His
85 90 95Thr Gly Glu Pro Val Tyr Asn Ala Ile Val Trp Gln Asp Thr Arg Thr
100 105 110Ser Asp Ile Cys Leu Glu Ile Ala Gly Glu Glu Gly Gln Glu Lys Trp
115 120 125Leu Asp Arg Thr Gly Leu Leu Ile Asn Ser Tyr Pro Ser Gly Pro Lys
130 135 140Ile Lys Trp Ile Leu Asp Asn Val Glu Gly Ala Arg Glu Arg Ala Glu145 150 155 160Lys Gly Asp Leu Leu Phe Gly Thr Met Asp Thr Trp Val Leu Trp Asn
165 170 175Leu Thr Gly Gly Val Arg Gly Asp Asp Gly Asp Asp Ala Ile His Val
180 185 190Thr Asp Val Thr Asn Ala Ser Arg Thr Leu Leu Met Asp Leu Arg Thr
195 200 205Gln Gln Trp Asp Pro Glu Leu Cys Glu Ala Leu Asp Ile Pro Met Ser
210 215 220Met Leu Pro Glu Ile Arg Pro Ser Val Gly Glu Phe Arg Ser Val Arg225 230 235 240His Arg Gly Thr Leu Ala Asp Val Pro Ile Thr Gly Val Leu Gly Asp
245 250 255Gln Gln Ala Ala Leu Phe Gly Gln Gly Gly Phe His Glu Gly Ala Ala
260 265 270Lys Asn Thr Tyr Gly Thr Gly Leu Phe Leu Leu Met Asn Thr Gly Thr
275 280 285Ser Leu Lys Ile Ser Glu His Gly Leu Leu Ser Thr Ile Ala Tyr Gln
290 295 300Arg Glu Gly Ser Ala Pro Val Tyr Ala Leu Glu Gly Ser Val Ser Met305 310 315 320Gly Gly Ser Leu Val Gln Trp Leu Arg Asp Asn Leu Gln Leu Ile Pro
325 330 335Asn Ala Pro Ala Ile Glu Asn Leu Ala Arg Glu Val Glu Asp Asn Gly
340 345 350Gly Val His Val Val Pro Ala Phe Thr Gly Leu Phe Ala Pro Arg Trp
355 360 365Arg Pro Asp Ala Arg Gly Val Ile Thr Gly Leu Thr Arg Phe Ala Asn
370 375 380Arg Lys His Ile Ala Arg Ala Val Leu Glu Ala Asn Ala Phe Gln Thr385 390 395 400Arg Glu Val Val Asp Ala Met Ala Lys Asp Ala Gly Lys Ala Leu Glu
405 410 415Ser Leu Arg Val Asp Gly Ala Met Val Glu Asn Asp Leu Leu Met Gln
420 425 430Met Gln Ala Asp Phe Leu Gly Ile Asp Val Gln Arg Leu Glu Asp Val
435 440 445Glu Thr Thr Ala Val Gly Val Ala Phe Ala Ala Gly Leu Gly Ser Gly
450 455 460Phe Phe Lys Thr Thr Asp Glu Ile Glu Lys Leu Ile Ala Val Lys Lys465 470 475 480Val Trp Asn Pro Asp Met Ser Glu Glu Glu Arg Glu Arg Arg Tyr Ala
485 490 495Glu Trp Asn Arg Ala Val Glu His Ser Tyr Asp Gln Ala
500 505<210>187<211>1119<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1096)<223>RXN01025<400>187gggcagcagc ggcaggtttc caggaggttt ccatgcgggt ggcttgggac atgggctaac 60ctgagacggt taaatatcgt tttcgaaagg tgggtttcgc gtg gtt tct gta agc 115
Val Val Ser Val Ser
1 5gtg atg ggt gca ggt tcc tgg gga acc acg ttg gcc aag gtc ttc tct 163Val Met Gly Ala Gly Ser Trp Gly Thr Thr Leu Ala Lys Val Phe Ser
10 15 20gat gct ggc aac gct gtg acg ttg tgg gcg agg cgg gaa gag ttg gca 211Asp Ala Gly Asn Ala Val Thr Leu Trp Ala Arg Arg Glu Glu Leu Ala
25 30 35agc acc atc cgt gac agc cat gaa aac cgt gat tac ctt ccg ggg att 259Ser Thr Ile Arg Asp Ser His Glu Asn Arg Asp Tyr Leu Pro Gly Ile
40 45 50acg ttg ccg gag tcg ctg cag gtc aca tca tcg gca acg gag gct tta 307Thr Leu Pro Glu Ser Leu Gln Val Thr Ser Ser Ala Thr Glu Ala Leu
55 60 65gag ggc gca gcc att gtg gtg ttg gcg att cct tcg cag gcg ttg cgt 355Glu Gly Ala Ala Ile Val Val Leu Ala Ile Pro Ser Gln Ala Leu Arg70 75 80 85ggc aat ttg gcg gag tgg aaa gag acg atc ccg cag gat gcg acc ttg 403Gly Asn Leu Ala Glu Trp Lys Glu Thr Ile Pro Gln Asp Ala Thr Leu
90 95 100gtg tcc ttg gct aaa ggt att gaa aag ggc acg cac ctg cgg atg agt 451Val Ser Leu Ala Lys Gly Ile Glu Lys Gly Thr His Leu Arg Met Ser
105 110 115gaa gtg atc gcg gag gtg acg gaa gcg gat cct tca cgc atc gcg gtg 499Glu Val Ile Ala Glu Val Thr Glu Ala Asp Pro Ser Arg Ile Ala Val
120 125 130ttg tcg ggg cca aac ctt gct cgt gag atc gcg gag ggg cag cct gca 547Leu Ser Gly Pro Asn Leu Ala Arg Glu Ile Ala Glu Gly Gln Pro Ala
135 140 145gct acg gtg att gct tgc cct gat gaa aac cga gcg aaa ctt gtg cag 595Ala Thr Val Ile Ala Cys Pro Asp Glu Asn Arg Ala Lys Leu Val Gln150 155 160 165gct gca gtg gct gcg ccg tat ttc cgc ccg tac acc aac act gat gtg 643Ala Ala Val Ala Ala Pro Tyr Phe Arg Pro Tyr Thr Asn Thr Asp Val
170 175 180gtg ggc act gaa atc ggt ggt gcg tgt aag aac gtc atc gcg ctg gcc 691Val Gly Thr Glu Ile Gly Gly Ala Cys Lys Asn Val Ile Ala Leu Ala
185 190 195tgt ggt att tcc cat ggt tac ggc ctg ggt gag aac acc aat gca tcg 739Cys Gly Ile Ser His Gly Tyr Gly Leu Gly Glu Asn Thr Asn Ala Ser
200 205 210ttg att act cgt ggc ctt gca gag atc gca cgc ctc ggt gcc aca ttg 787Leu Ile Thr Arg Gly Leu Ala Glu Ile Ala Arg Leu Gly Ala Thr Leu
215 220 225ggt gcg gat gcg aag act ttt tct ggc ctt gcg gga atg ggc gac ttg 835Gly Ala Asp Ala Lys Thr Phe Ser Gly Leu Ala Gly Met Gly Asp Leu230 235 240 245gtg gct acg tgt tca tca ccg ctg tcg cgt aac cgc agc ttc ggt gag 883Val Ala Thr Cys Ser Ser Pro Leu Ser Arg Asn Arg Ser Phe Gly Glu
250 255 260cgt ttg ggt cag ggt gaa tcc cta gag aag gct cgc gag gca acc aat 931Arg Leu Gly Gln Gly Glu Ser Leu Glu Lys Ala Arg Glu Ala Thr Asn
265 270 275ggt cag gtt gcg gag ggt gtt att tcc tcg cag tcg att ttt gat ctt 979Gly Gln Val Ala Glu Gly Val Ile Ser Ser Gln Ser Ile Phe Asp Leu
280 285 290gcc acc aag ctt ggt gtg gag atg ccg atc acc cag gct gtc tac ggt 1027Ala Thr Lys Leu Gly Val Glu Met Pro Ile Thr Gln Ala Val Tyr Gly
295 300 305gtg tgc cac cga gat atg aaa gta act gac atg att gtg gct ctc atg 1075Val Cys His Arg Asp Met Lys Val Thr Asp Met Ile Val Ala Leu Met310 315 320 325ggc agg tct aag aag gct gag tagtcttagg ttgtaagctt caa 1119Gly Arg Ser Lys Lys Ala Glu
330<210>188<211>332<212>PRT<213>谷氨酸棒杆菌<400>188Val Val Ser Val Ser Val Met Gly Ala Gly Ser Trp Gly Thr Thr Leu1 5 10 15Ala Lys Val Phe Ser Asp Ala Gly Asn Ala Val Thr Leu Trp Ala Arg
20 25 30Arg Glu Glu Leu Ala Ser Thr Ile Arg Asp Ser His Glu Asn Arg Asp
35 40 45Tyr Leu Pro Gly Ile Thr Leu Pro Glu Ser Leu Gln Val Thr Ser Ser
50 55 60Ala Thr Glu Ala Leu Glu Gly Ala Ala Ile Val Val Leu Ala Ile Pro65 70 75 80Ser Gln Ala Leu Arg Gly Asn Leu Ala Glu Trp Lys Glu Thr Ile Pro
85 90 95Gln Asp Ala Thr Leu Val Ser Leu Ala Lys Gly Ile Glu Lys Gly Thr
100 105 110His Leu Arg Met Ser Glu Val Ile Ala Glu Val Thr Glu Ala Asp Pro
115 120 125Ser Arg Ile Ala Val Leu Ser Gly Pro Asn Leu Ala Arg Glu Ile Ala
130 135 140Glu Gly Gln Pro Ala Ala Thr Val Ile Ala Cys Pro Asp Glu Asn Arg145 150 155 160Ala Lys Leu Val Gln Ala Ala Val Ala Ala Pro Tyr Phe Arg Pro Tyr
165 170 175Thr Asn Thr Asp Val Val Gly Thr Glu Ile Gly Gly Ala Cys Lys Asn
180 185 190Val Ile Ala Leu Ala Cys Gly Ile Ser His Gly Tyr Gly Leu Gly Glu
195 200 205Asn Thr Asn Ala Ser Leu Ile Thr Arg Gly Leu Ala Glu Ile Ala Arg
210 215 220Leu Gly Ala Thr Leu Gly Ala Asp Ala Lys Thr Phe Ser Gly Leu Ala225 230 235 240Gly Met Gly Asp Leu Val Ala Thr Cys Ser Ser Pro Leu Ser Arg Asn
245 250 255Arg Ser Phe Gly Glu Arg Leu Gly Gln Gly Glu Ser Leu Glu Lys Ala
260 265 270Arg Glu Ala Thr Asn Gly Gln Val Ala Glu Gly Val Ile Ser Ser Gln
275 280 285Ser Ile Phe Asp Leu Ala Thr Lys Leu Gly Val Glu Met Pro Ile Thr
290 295 300Gln Ala Val Tyr Gly Val Cys His Arg Asp Met Lys Val Thr Asp Met305 310 315 320Ile Val Ala Leu Met Gly Arg Ser Lys Lys Ala Glu
325 330<210>189<211>1015<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1015)<223>FRXA01025<400>189gggcagcagc ggcaggtttc cagraggttt ccatgcgggt ggcttggrac wtgggctaac 60ctgaracggt taaatatcgt tttcgaaagg tgggtttcgc gtg gtt tct gta agc 115
Val Val Ser Val Ser
1 5gtg atg ggt gca ggt tcc tgg gga acc acg ttg gcc aag gtc ttc tct 163Val Met Gly Ala Gly Ser Trp Gly Thr Thr Leu Ala Lys Val Phe Ser
10 15 20gat gct ggc aac gct gtg acg ttg tgg gcg agg cgg gaa gag ttg gca 211Asp Ala Gly Asn Ala Val Thr Leu Trp Ala Arg Arg Glu Glu Leu Ala
25 30 35agc acc atc cgt gac agc cat gaa aac cgt gat tac ctt ccg ggg att 259Ser Thr Ile Arg Asp Ser His Glu Asn Arg Asp Tyr Leu Pro Gly Ile
40 45 50acg ttg ccg gag tcg ctg cag gtc aca tca tcg gca acg gag gct tta 307Thr Leu Pro Glu Ser Leu Gln Val Thr Ser Ser Ala Thr Glu Ala Leu
55 60 65gag ggc gca gcc att gtg gtg ttg gcg att cct tcg cag gcg ttg cgt 355Glu Gly Ala Ala Ile Val Val Leu Ala Ile Pro Ser Gln Ala Leu Arg70 75 80 85ggc aat ttg gcg gag tgg aaa gag acg atc ccg cag gat gcg acc ttg 403Gly Asn Leu Ala Glu Trp Lys Glu Thr Ile Pro Gln Asp Ala Thr Leu
90 95 100gtg tcc ttg gct aaa ggt att gaa aag ggc acg cac ctg cgg atg agt 451Val Ser Leu Ala Lys Gly Ile Glu Lys Gly Thr His Leu Arg Met Ser
105 110 115gaa gtg atc gcg gag gtg acg gaa gcg gat cct tca cgc atc gcg gtg 499Glu Val Ile Ala Glu Val Thr Glu Ala Asp Pro Ser Arg Ile Ala Val
120 125 130ttg tcg ggg cca aac ctt gct cgt gag atc gcg gag ggg cag cct gca 547Leu Ser Gly Pro Asn Leu Ala Arg Glu Ile Ala Glu Gly Gln Pro Ala
135 140 145gct acg gtg att gct tgc cct gat gaa aac cga gcg aaa ctt gtg cag 595Ala Thr Val Ile Ala Cys Pro Asp Glu Asn Arg Ala Lys Leu Val Gln150 155 160 165gct gca gtg gct gcg ccg tat ttc cgc ccg tac acc aac act gat gtg 643Ala Ala Val Ala Ala Pro Tyr Phe Arg Pro Tyr Thr Asn Thr Asp Val
170 175 180gtg ggc act gaa atc ggt ggt gcg tgt aag aac gtc atc gcg ctg gcc 691Val Gly Thr Glu Ile Gly Gly Ala Cys Lys Asn Val Ile Ala Leu Ala
185 190 195tgt ggt att tcc cat ggt tac ggc ctg ggt gag aac acc aat gca tcg 739Cys Gly Ile Ser His Gly Tyr Gly Leu Gly Glu Asn Thr Asn Ala Ser
200 205 210ttg att act cgt ggc ctt gca gag atc gca cgc ctc ggt gcc aca ttg 787Leu Ile Thr Arg Gly Leu Ala Glu Ile Ala Arg Leu Gly Ala Thr Leu
215 220 225ggt gcg gat gcg aag act ttt tct ggc ctt gcg gga atg ggc gac ttg 835Gly Ala Asp Ala Lys Thr Phe Ser Gly Leu Ala Gly Met Gly Asp Leu230 235 240 245gtg gct acg tgt tca tca ccg ctg tcg cgt aac cgc agc ttc ggt gag 883Val Ala Thr Cys Ser Ser Pro Leu Ser Arg Asn Arg Ser Phe Gly Glu
250 255 260cgt ttg ggt cag ggt gaa tcc cta gag aag gct cgc gag gca acc aat 931Arg Leu Gly Gln Gly Glu Ser Leu Glu Lys Ala Arg Glu Ala Thr Asn
265 270 275ggt cag gtt gcg gag ggt gtt att tcc tcg cag tcg att ttt gat ctt 979Gly Gln Val Ala Glu Gly Val Ile Ser Ser Gln Ser Ile Phe Asp Leu
280 285 290gcc acc aag ctt ggt gtg gag atg ccg atc acc cag 1015Ala Thr Lys Leu Gly Val Glu Met Pro Ile Thr Gln
295 300 305<210>190<211>305<212>PRT<213>谷氨酸棒杆菌<400>190Val Val Ser Val Ser Val Met Gly Ala Gly Ser Trp Gly Thr Thr Leu1 5 10 15Ala Lys Val Phe Ser Asp Ala Gly Asn Ala Val Thr Leu Trp Ala Arg
20 25 30Arg Glu Glu Leu Ala Ser Thr Ile Arg Asp Ser His Glu Asn Arg Asp
35 40 45Tyr Leu Pro Gly Ile Thr Leu Pro Glu Ser Leu Gln Val Thr Ser Ser
50 55 60Ala Thr Glu Ala Leu Glu Gly Ala Ala Ile Val Val Leu Ala Ile Pro65 70 75 80Ser Gln Ala Leu Arg Gly Asn Leu Ala Glu Trp Lys Glu Thr Ile Pro
85 90 95Gln Asp Ala Thr Leu Val Ser Leu Ala Lys Gly Ile Glu Lys Gly Thr
100 105 110His Leu Arg Met Ser Glu Val Ile Ala Glu Val Thr Glu Ala Asp Pro
115 120 125Ser Arg Ile Ala Val Leu Ser Gly Pro Asn Leu Ala Arg Glu Ile Ala
130 135 140Glu Gly Gln Pro Ala Ala Thr Val Ile Ala Cys Pro Asp Glu Asn Arg145 150 155 160Ala Lys Leu Val Gln Ala Ala Val Ala Ala Pro Tyr Phe Arg Pro Tyr
165 170 175Thr Asn Thr Asp Val Val Gly Thr Glu Ile Gly Gly Ala Cys Lys Asn
180 185 190Val Ile Ala Leu Ala Cys Gly Ile Ser His Gly Tyr Gly Leu Gly Glu
195 200 205Asn Thr Asn Ala Ser Leu Ile Thr Arg Gly Leu Ala Glu Ile Ala Arg
210 215 220Leu Gly Ala Thr Leu Gly Ala Asp Ala Lys Thr Phe Ser Gly Leu Ala225 230 235 240Gly Met Gly Asp Leu Val Ala Thr Cys Ser Ser Pro Leu Ser Arg Asn
245 250 255Arg Ser Phe Gly Glu Arg Leu Gly Gln Gly Glu Ser Leu Glu Lys Ala
260 265 270Arg Glu Ala Thr Asn Gly Gln Val Ala Glu Gly Val Ile Ser Ser Gln
275 280 285Ser Ile Phe Asp Leu Ala Thr Lys Leu Gly Val Glu Met Pro Ile Thr
290 295 300Gln305<210>191<211>1809<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1786)<223>RXA01851<400>191ttgtggcctt tttgcagggg aaacttattt aaataattca taagtaaaaa accgtcaatt 60cacgatgtgg gttggcggtt ttcctattag gctcactttt atg acg agc gca cac 115
Met Thr Ser Ala His
1 5ttt gaa tcc cgc cgg att ggc cct ccg ctt cgc gat aat tat gac gtc 163Phe Glu Ser Arg Arg Ile Gly Pro Pro Leu Arg Asp Asn Tyr Asp Val
10 15 20att gtg att ggc ggt ggt atc tca ggt gta cag att gcg cga cat gct 211Ile Val Ile Gly Gly Gly Ile Ser Gly Val Gln Ile Ala Arg His Ala
25 30 35caa ggc cgc ggt tta cgc act gtg atg ttt gag gcc aga gat tat tct 259Gln Gly Arg Gly Leu Arg Thr Val Met Phe Glu Ala Arg Asp Tyr Ser
40 45 50tct gga aca tca tcg aca acc tcc aag atg att cat ggt ggt ttg cgc 307Ser Gly Thr Ser Ser Thr Thr Ser Lys Met Ile His Gly Gly Leu Arg
55 60 65tat ttg gag cag tac gat ttc ggc gtg gtc cag gaa gcc gtg aag gaa 355Tyr Leu Glu Gln Tyr Asp Phe Gly Val Val Gln Glu Ala Val Lys Glu70 75 80 85cgc cgg tac cta ggt atc gcc gct ccg cat ttg gtg gct cca cgc agt 403Arg Arg Tyr Leu Gly Ile Ala Ala Pro His Leu Val Ala Pro Arg Ser
90 95 100ttc atg ctc acg gcg ttt gat tgg tca gag ccg aaa gcc cct atg ttg 451Phe Met Leu Thr Ala Phe Asp Trp Ser Glu Pro Lys Ala Pro Met Leu
105 110 115ggt gct ggt gtg gcg ttg tat gaa acg atg gcg tgg cag cgt aac cag 499Gly Ala Gly Val Ala Leu Tyr Glu Thr Met Ala Trp Gln Arg Asn Gln
120 125 130ggg caa tcg aag gaa aac cac tcg ccg cgt ttc cgg tgg att cct aaa 547Gly Gln Ser Lys Glu Asn His Ser Pro Arg Phe Arg Trp Ile Pro Lys
135 140 145aat gca ctg ctc aag gaa gtc ccg tgg ctt gac ccg gag ggc ttg aag 595Asn Ala Leu Leu Lys Glu Val Pro Trp Leu Asp Pro Glu Gly Leu Lys150 155 160 165gga gcg tgg cgc cac gat gat acg ttg aat ctc cat gca gaa cga ctc 643Gly Ala Trp Arg His Asp Asp Thr Leu Asn Leu His Ala Glu Arg Leu
170 175 180ctc ctc gcg gtg att aaa gct ttt gcg gca gat ggc gga acg gcg atc 691Leu Leu Ala Val Ile Lys Ala Phe Ala Ala Asp Gly Gly Thr Ala Ile
185 190 195aac cac gcc aaa gtc act cgc att ctc cgg aac gtg gaa gaa ggc cgc 739Asn His Ala Lys Val Thr Arg Ile Leu Arg Asn Val Glu Glu Gly Arg
200 205 210gtc aag ggt gta gaa gtg act gat cag gtc acc aac acc acg cat gag 787Val Lys Gly Val Glu Val Thr Asp Gln Val Thr Asn Thr Thr His Glu
215 220 225gtg aat gcc cct gtg gtg atc aac gct gcg ggt ccg tgg gtt gcg cag 835Val Asn Ala Pro Val Val Ile Asn Ala Ala Gly Pro Trp Val Ala Gln230 235 240 245gcg ttg ggt gat ttg gcg gag gta acc aag ttg aag gtg cgc caa tcc 883Ala Leu Gly Asp Leu Ala Glu Val Thr Lys Leu Lys Val Arg Gln Ser
250 255 260aag gga gtg cat ttg ctc act ggt gat ttg ggc agc cag agt ggc gtg 931Lys Gly Val His Leu Leu Thr Gly Asp Leu Gly Ser Gln Ser Gly Val
265 270 275ttt gtg cgt ggc aaa aac ggc aag cat gtg atc gtg aat ccg tgg atg 979Phe Val Arg Gly Lys Asn Gly Lys His Val Ile Val Asn Pro Trp Met
280 285 290ggg cgc acc ctt att ggt cca acc gac acc atg att gac ggt gac gct 1027Gly Arg Thr Leu Ile Gly Pro Thr Asp Thr Met Ile Asp Gly Asp Ala
295 300 305gat gat gcg gct gca gat gaa agc gat atc gat ttg ctg ctt gag acc 1075Asp Asp Ala Ala Ala Asp Glu Ser Asp Ile Asp Leu Leu Leu Glu Thr310 315 320 325atc gat tcg gta cgc gct aca ccg ctt gat cgc aaa gag atc atc tcc 1123Ile Asp Ser Val Arg Ala Thr Pro Leu Asp Arg Lys Glu Ile Ile Ser
330 335 340acg ctg gtg ggt gtg cgc ccg ctt gtt gat gac ggc acc gac acc tac 1171Thr Leu Val Gly Val Arg Pro Leu Val Asp Asp Gly Thr Asp Thr Tyr
345 350 355acg tcc tct cgc cgt ttc gat att tcc gat cac gcc aac gtc ggc att 1219Thr Ser Ser Arg Arg Phe Asp Ile Ser Asp His Ala Asn Val Gly Ile
360 365 370gat ggt ttg gtg tct gtc tct ggc ggc aag tgg acc act tcc cgc gtg 1267Asp Gly Leu Val Ser Val Ser Gly Gly Lys Trp Thr Thr Ser Arg Val
375 380 385atg ggg tac aag gtg att gag cat gtg gtg gag cac caa gct gcg gtg 1315Met Gly Tyr Lys Val Ile Glu His Val Val Glu His Gln Ala Ala Val390 395 400 405tta cct ccg ctg cgc cac ttt gac tcc agg cag atg ccg ttg agt act 1363Leu Pro Pro Leu Arg His Phe Asp Ser Arg Gln Met Pro Leu Ser Thr
410 415 420tct ttt ggc gcg tat gag tcc gtg gct gat tcc ttt gag tca gcg ctt 1411Ser Phe Gly Ala Tyr Glu Ser Val Ala Asp Ser Phe Glu Ser Ala Leu
425 430 435cgc agc cac ccc gag ctg gat gtg gat gat gaa atc cgc gtg cat ctg 1459Arg Ser His Pro Glu Leu Asp Val Asp Asp Glu Ile Arg Val His Leu
440 445 450gcc aga ctg tat gga act gag cat gaa aaa gtg ctg gat ctc gtc gca 1507Ala Arg Leu Tyr Gly Thr Glu His Glu Lys Val Leu Asp Leu Val Ala
455 460 465aag caa ccc gac ctg ggg cgc cga ctt gac cca gac aac ctt gat atc 1555Lys Gln Pro Asp Leu Gly Arg Arg Leu Asp Pro Asp Asn Leu Asp Ile470 475 480 485gcg gcg cag gcc gtt ttt gct gtc gcc gag gag gcg gcc gtc gac ctg 1603Ala Ala Gln Ala Val Phe Ala Val Ala Glu Glu Ala Ala Val Asp Leu
490 495 500gcg gac gtg ctg gat cgt cgc atc gtg ctc ggc acg ctg ggt tat gtg 1651Ala Asp Val Leu Asp Arg Arg Ile Val Leu Gly Thr Leu Gly Tyr Val
505 510 515caa ccg gct gcc gtg cgt gcg acg gcc gaa gca atg gcg cag gtc acc 1699Gln Pro Ala Ala Val Arg Ala Thr Ala Glu Ala Met Ala Gln Val Thr
520 525 530ggg tgg tca gct gag ctt atc gac gcc cag tgc cag tcc tac ctc gcc 1747Gly Trp Ser Ala Glu Leu Ile Asp Ala Gln Cys Gln Ser Tyr Leu Ala
535 540 545aag caa gac aaa atc caa gcc gtg tta aag ccg tac cgc taacactccg 1796Lys Gln Asp Lys Ile Gln Ala Val Leu Lys Pro Tyr Arg550 555 560tcatcgacac cgg 1809<210>192<211>562<212>PRT<213>谷氨酸棒杆菌<400>192Met Thr Ser Ala His Phe Glu Ser Arg Arg Ile Gly Pro Pro Leu Arg1 5 10 15Asp Asn Tyr Asp Val Ile Val Ile Gly Gly Gly Ile Ser Gly Val Gln
20 25 30Ile Ala Arg His Ala Gln Gly Arg Gly Leu Arg Thr Val Met Phe Glu
35 40 45Ala Arg Asp Tyr Ser Ser Gly Thr Ser Ser Thr Thr Ser Lys Met Ile
50 55 60His Gly Gly Leu Arg Tyr Leu Glu Gln Tyr Asp Phe Gly Val Val Gln65 70 75 80Glu Ala Val Lys Glu Arg Arg Tyr Leu Gly Ile Ala Ala Pro His Leu
85 90 95Val Ala Pro Arg Ser Phe Met Leu Thr Ala Phe Asp Trp Ser Glu Pro
100 105 110Lys Ala Pro Met Leu Gly Ala Gly Val Ala Leu Tyr Glu Thr Met Ala
115 120 125Trp Gln Arg Asn Gln Gly Gln Ser Lys Glu Asn His Ser Pro Arg Phe
130 135 140Arg Trp Ile Pro Lys Asn Ala Leu Leu Lys Glu Val Pro Trp Leu Asp145 150 155 160Pro Glu Gly Leu Lys Gly Ala Trp Arg His Asp Asp Thr Leu Asn Leu
165 170 175His Ala Glu Arg Leu Leu Leu Ala Val Ile Lys Ala Phe Ala Ala Asp
180 185 190Gly Gly Thr Ala Ile Asn His Ala Lys Val Thr Arg Ile Leu Arg Asn
195 200 205Val Glu Glu Gly Arg Val Lys Gly Val Glu Val Thr Asp Gln Val Thr
210 215 220Asn Thr Thr His Glu Val Asn Ala Pro Val Val Ile Asn Ala Ala Gly225 230 235 240Pro Trp Val Ala Gln Ala Leu Gly Asp Leu Ala Glu Val Thr Lys Leu
245 250 255Lys Val Arg Gln Ser Lys Gly Val His Leu Leu Thr Gly Asp Leu Gly
260 265 270Ser Gln Ser Gly Val Phe Val Arg Gly Lys Asn Gly Lys His Val Ile
275 280 285Val Asn Pro Trp Met Gly Arg Thr Leu Ile Gly Pro Thr Asp Thr Met
290 295 300Ile Asp Gly Asp Ala Asp Asp Ala Ala Ala Asp Glu Ser Asp Ile Asp305 310 315 320Leu Leu Leu Glu Thr Ile Asp Ser Val Arg Ala Thr Pro Leu Asp Arg
325 330 335Lys Glu Ile Ile Ser Thr Leu Val Gly Val Arg Pro Leu Val Asp Asp
340 345 350Gly Thr Asp Thr Tyr Thr Ser Ser Arg Arg Phe Asp Ile Ser Asp His
355 360 365Ala Asn Val Gly Ile Asp Gly Leu Val Ser Val Ser Gly Gly Lys Trp
370 375 380Thr Thr Ser Arg Val Met Gly Tyr Lys Val Ile Glu His Val Val Glu385 390 395 400His Gln Ala Ala Val Leu Pro Pro Leu Arg His Phe Asp Ser Arg Gln
405 410 415Met Pro Leu Ser Thr Ser Phe Gly Ala Tyr Glu Ser Val Ala Asp Ser
420 425 430Phe Glu Ser Ala Leu Arg Ser His Pro Glu Leu Asp Val Asp Asp Glu
435 440 445Ile Arg Val His Leu Ala Arg Leu Tyr Gly Thr Glu His Glu Lys Val
450 455 460Leu Asp Leu Val Ala Lys Gln Pro Asp Leu Gly Arg Arg Leu Asp Pro465 470 475 480Asp Asn Leu Asp Ile Ala Ala Gln Ala Val Phe Ala Val Ala Glu Glu
485 490 495Ala Ala Val Asp Leu Ala Asp Val Leu Asp Arg Arg Ile Val Leu Gly
500 505 510Thr Leu Gly Tyr Val Gln Pro Ala Ala Val Arg Ala Thr Ala Glu Ala
515 520 525Met Ala Gln Val Thr Gly Trp Ser Ala Glu Leu Ile Asp Ala Gln Cys
530 535 540Gln Ser Tyr Leu Ala Lys Gln Asp Lys Ile Gln Ala Val Leu Lys Pro545 550 555 560Tyr Arg<210>193<211>900<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(877)<223>RXA01242<400>193cgccggcaac caaatgaggc ttttgggcgt tggacagtga gacaatgggt aagaaattcg 60gacatattta gtaaattggc tttttgcttt aaggagtgac atg tac gca gag gag 115
Met Tyr Ala Glu Glu
1 5cgc cgt cga cag att gcc tca tta acg gca gtt gag gga cgt gta aat 163Arg Arg Arg Gln Ile Ala Ser Leu Thr Ala Val Glu Gly Arg Val Asn
10 15 20gtc aca gaa tta gcg ggc cga ttc gat gtc act gca gag acg att cga 211Val Thr Glu Leu Ala Gly Arg Phe Asp Val Thr Ala Glu Thr Ile Arg
25 30 35cga gac ctt gcg gtg cta gac cgc gag gga att gtt cac cgc gtt cac 259Arg Asp Leu Ala Val Leu Asp Arg Glu Gly Ile Val His Arg Val His
40 45 50ggt ggc gca gta gcc acc caa tct ttc caa acc aca gag ttg agc ttg 307Gly Gly Ala Val Ala Thr Gln Ser Phe Gln Thr Thr Glu Leu Ser Leu
55 60 65gat act cgt ttc agg tct gca tcg tca gca aag tac tcc att gcc aag 355Asp Thr Arg Phe Arg Ser Ala Ser Ser Ala Lys Tyr Ser Ile Ala Lys70 75 80 85gca gcg atg cag ttc ctg ccc gct gag cat ggc gga ctg ttc ctc gat 403Ala Ala Met Gln Phe Leu Pro Ala Glu His Gly Gly Leu Phe Leu Asp
90 95 100gcg gga act act gtt act gct ttg gcc gat ctc att tct gag cat cct 451Ala Gly Thr Thr Val Thr Ala Leu Ala Asp Leu Ile Ser Glu His Pro
105 110 115agc tcc aag cag tgg tcg atc gtg acc aac tgc ctc ccc atc gca ctt 499Ser Ser Lys Gln Trp Ser Ile Val Thr Asn Cys Leu Pro Ile Ala Leu
120 125 130aat ctg gcc aac gcc ggg ctt gat gat gtc cag ctg ctt gga gga agc 547Asn Leu Ala Asn Ala Gly Leu Asp Asp Val Gln Leu Leu Gly Gly Ser
135 140 145gtt cgc gcg atc acc cag gct gtt gtg ggt gac act gcg ctt cgt act 595Val Arg Ala Ile Thr Gln Ala Val Val Gly Asp Thr Ala Leu Arg Thr150 155 160 165ctc gcg ctg atg cgt gcg gat gta gtg ttc atc ggc acc aac gcg ttg 643Leu Ala Leu Met Arg Ala Asp Val Val Phe Ile Gly Thr Asn Ala Leu
170 175 180acg ttg gat cac gga ttg tct acg gcc gat tcc caa gag gct gcc atg 691Thr Leu Asp His Gly Leu Ser Thr Ala Asp Ser Gln Glu Ala Ala Met
185 190 195aaa tct gcg atg atc acc aac gcc cac aag gtg gtg gtg ttg tgt gac 739Lys Ser Ala Met Ile Thr Asn Ala His Lys Val Val Val Leu Cys Asp
200 205 210tcc acc aag atg ggc acc gac tac ctc gtg agc ttt ggc gca atc agc 787Ser Thr Lys Met Gly Thr Asp Tyr Leu Val Ser Phe Gly Ala Ile Ser
215 220 225gat atc gat gtg gtg gtc acc gat gcg ggt gca cca gca agt ttc gtt 835Asp Ile Asp Val Val Val Thr Asp Ala Gly Ala Pro Ala Ser Phe Val230 235 240 245gag cag ttg cga gaa cgc gat gta gaa gtt gtg att gca gaa 877Glu Gln Leu Arg Glu Arg Asp Val Glu Val Val Ile Ala Glu
250 255tgattcttac agtcactgca agt 900<210>194<211>259<212>PRT<213>谷氨酸棒杆菌<400>194Met Tyr Ala Glu Glu Arg Arg Arg Gln Ile Ala Ser Leu Thr Ala Val1 5 10 15Glu Gly Arg Val Asn Val Thr Glu Leu Ala Gly Arg Phe Asp Val Thr
20 25 30Ala Glu Thr Ile Arg Arg Asp Leu Ala Val Leu Asp Arg Glu Gly Ile
35 40 45Val His Arg Val His Gly Gly Ala Val Ala Thr Gln Ser Phe Gln Thr
50 55 60Thr Glu Leu Ser Leu Asp Thr Arg Phe Arg Ser Ala Ser Ser Ala Lys65 70 75 80Tyr Ser Ile Ala Lys Ala Ala Met Gln Phe Leu Pro Ala Glu His Gly
85 90 95Gly Leu Phe Leu Asp Ala Gly Thr Thr Val Thr Ala Leu Ala Asp Leu
100 105 110Ile Ser Glu His Pro Ser Ser Lys Gln Trp Ser Ile Val Thr Asn Cys
115 120 125Leu Pro Ile Ala Leu Asn Leu Ala Asn Ala Gly Leu Asp Asp Val Gln
130 135 140Leu Leu Gly Gly Ser Val Arg Ala Ile Thr Gln Ala Val Val Gly Asp145 150 155 160Thr Ala Leu Arg Thr Leu Ala Leu Met Arg Ala Asp Val Val Phe Ile
165 170 175Gly Thr Asn Ala Leu Thr Leu Asp His Gly Leu Ser Thr Ala Asp Ser
180 185 190Gln Glu Ala Ala Met Lys Ser Ala Met Ile Thr Asn Ala His Lys Val
195 200 205Val Val Leu Cys Asp Ser Thr Lys Met Gly Thr Asp Tyr Leu Val Ser
210 215 220Phe Gly Ala Ile Ser Asp Ile Asp Val Val Val Thr Asp Ala Gly Ala225 230 235 240Pro Ala Ser Phe Val Glu Gln Leu Arg Glu Arg Asp Val Glu Val Val
245 250 255Ile Ala Glu<210>195<211>969<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(946)<223>RXA02288<400>195aacaacaatc taacgccatc atgttataaa aaagcaagac ctaacataaa aatgttagaa 60agtgctggat ctaacaacat ttccgtggta actttttcac atg tcc caa gtg att 115
Met Ser Gln Val Ile
1 5ccc gcc agc tca caa gaa aag cgt cgt gag cgc atc gtt tct tat gtc 163Pro Ala Ser Ser Gln Glu Lys Arg Arg Glu Arg Ile Val Ser Tyr Val
10 15 20acc cgt cat gga ttc gct cgt gtt gaa gca tta gct gag ctt ttt gag 211Thr Arg His Gly Phe Ala Arg Val Glu Ala Leu Ala Glu Leu Phe Glu
25 30 35gtc agc gca atg acc att cac cgt gat ttg gag gcg ctg gct gca gac 259Val Ser Ala Met Thr Ile His Arg Asp Leu Glu Ala Leu Ala Ala Asp
40 45 50aat ttg gtg gag cgc att agg ggt ggc gcg cgt tcg gtg tcg ccg tcg 307Asn Leu Val Glu Arg Ile Arg Gly Gly Ala Arg Ser Val Ser Pro Ser
55 60 65atg agt gag ttg gca gtg gag cag cgt cgg cat ttg cat cgc act gtt 355Met Ser Glu Leu Ala Val Glu Gln Arg Arg His Leu His Arg Thr Val70 75 80 85aaa gag gcg ttg tgt act gca gca gca cgg ttg att ccg gag ggc gct 403Lys Glu Ala Leu Cys Thr Ala Ala Ala Arg Leu Ile Pro Glu Gly Ala
90 95 100gtg gtg gcg att gat gat tcc acc acg ttg gag tct ttg gtt gag aag 451Val Val Ala Ile Asp Asp Ser Thr Thr Leu Glu Ser Leu Val Glu Lys
105 110 115ttg ccg cag cgg tca cca tcg gcg ttg att acg cat tct ttg aag aca 499Leu Pro Gln Arg Ser Pro Ser Ala Leu Ile Thr His Ser Leu Lys Thr
120 125 130atg gcg gat cat cgt gtg cgc gcc ggg atg agc gat atc cgt ttg att 547Met Ala Asp His Arg Val Arg Ala Gly Met Ser Asp Ile Arg Leu Ile
135 140 145gcg tgt gcg gga ttg tat ttc gcg gag act gat tct ttc ttg ggc aag 595Ala Cys Ala Gly Leu Tyr Phe Ala Glu Thr Asp Ser Phe Leu Gly Lys150 155 160 165gca act tca gcg cag ttg aat gag ctg tcg gcg gat att tct ttt gtt 643Ala Thr Ser Ala Gln Leu Asn Glu Leu Ser Ala Asp Ile Ser Phe Val
170 175 180tct acg act gcg gtg cgc gct acg ggg gag gtt ccg gcg ctg ttt cat 691Ser Thr Thr Ala Val Arg Ala Thr Gly Glu Val Pro Ala Leu Phe His
185 190 195cct gat atg gag gct gct gat acg aag cgg gcg ttg att ggg att ggt 739Pro Asp Met Glu Ala Ala Asp Thr Lys Arg Ala Leu Ile Gly Ile Gly
200 205 210agc gtg cgt gtg ttg gtg gtg gat tct agt aaa ttt ggt tcg gct ggt 787Ser Val Arg Val Leu Val Val Asp Ser Ser Lys Phe Gly Ser Ala Gly
215 220 225gtg ttc aag gtt gct tcg att gag gag ttt gac cac atc atc att gat 835Val Phe Lys Val Ala Ser Ile Glu Glu Phe Asp His Ile Ile Ile Asp230 235 240 245cag cag tgc acc cgt gag cag cgg gat ctt ttg cgt aat tcg cgc gcg 883Gln Gln Cys Thr Arg Glu Gln Arg Asp Leu Leu Arg Asn Ser Arg Ala
250 255 260cag atc cat gtg att gac cac aat ggt gat gaa att ttg gat acc cca 931Gln Ile His Val Ile Asp His Asn Gly Asp Glu Ile Leu Asp Thr Pro
265 270 275acg gaa gag gat ttt taagatggct ttggttcttg gaa 969Thr Glu Glu Asp Phe
280<210>196<211>282<212>PRT<213>谷氨酸棒杆菌<400>196Met Ser Gln Val Ile Pro Ala Ser Ser Gln Glu Lys Arg Arg Glu Arg1 5 10 15Ile Val Ser Tyr Val Thr Arg His Gly Phe Ala Arg Val Glu Ala Leu
20 25 30Ala Glu Leu Phe Glu Val Ser Ala Met Thr Ile His Arg Asp Leu Glu
35 40 45Ala Leu Ala Ala Asp Asn Leu Val Glu Arg Ile Arg Gly Gly Ala Arg
50 55 60Ser Val Ser Pro Ser Met Ser Glu Leu Ala Val Glu Gln Arg Arg His65 70 75 80Leu His Arg Thr Val Lys Glu Ala Leu Cys Thr Ala Ala Ala Arg Leu
85 90 95Ile Pro Glu Gly Ala Val Val Ala Ile Asp Asp Ser Thr Thr Leu Glu
100 105 110Ser Leu Val Glu Lys Leu Pro Gln Arg Ser Pro Ser Ala Leu Ile Thr
115 120 125His Ser Leu Lys Thr Met Ala Asp His Arg Val Arg Ala Gly Met Ser
130 135 140Asp Ile Arg Leu Ile Ala Cys Ala Gly Leu Tyr Phe Ala Glu Thr Asp145 150 155 160Ser Phe Leu Gly Lys Ala Thr Ser Ala Gln Leu Asn Glu Leu Ser Ala
165 170 175Asp Ile Ser Phe Val Ser Thr Thr Ala Val Arg Ala Thr Gly Glu Val
180 185 190Pro Ala Leu Phe His Pro Asp Met Glu Ala Ala Asp Thr Lys Arg Ala
195 200 205Leu Ile Gly Ile Gly Ser Val Arg Val Leu Val Val Asp Ser Ser Lys
210 215 220Phe Gly Ser Ala Gly Val Phe Lys Val Ala Ser Ile Glu Glu Phe Asp225 230 235 240His Ile Ile Ile Asp Gln Gln Cys Thr Arg Glu Gln Arg Asp Leu Leu
245 250 255Arg Asn Ser Arg Ala Gln Ile His Val Ile Asp His Asn Gly Asp Glu
260 265 270Ile Leu Asp Thr Pro Thr Glu Glu Asp Phe
275 280<210>197<211>887<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(864)<223>RXN01891<400>197ggt ggc cac tat ggt ttg cct ttc gct cgc tcc act gtc ctc ttc tac 48Gly Gly His Tyr Gly Leu Pro Phe Ala Arg Ser Thr Val Leu Phe Tyr1 5 10 15tac aac aag gat ctg tgg gct aag gct ggc ctg gaa gat cgt ggc cca 96Tyr Asn Lys Asp Leu Trp Ala Lys Ala Gly Leu Glu Asp Arg Gly Pro
20 25 30gag tca tgg gaa gag ttc tcc gag tgg ggt cca aag ctg cag gaa gcg 144Glu Ser Trp Glu Glu Phe Ser Glu Trp Gly Pro Lys Leu Gln Glu Ala
35 40 45atg gac agt ggt ttc gca cac ggt tgg gga gat gca acc aac tac ctt 192Met Asp Ser Gly Phe Ala His Gly Trp Gly Asp Ala Thr Asn Tyr Leu
50 55 60tct tgg act ttc gaa ggc cca atg tgg tcc ctc ggc ggc aac tac tct 240Ser Trp Thr Phe Glu Gly Pro Met Trp Ser Leu Gly Gly Asn Tyr Ser65 70 75 80gaa ggt tgg gag tcc cgt ctg act acc cca gag acc atc cgt gca gtt 288Glu Gly Trp Glu Ser Arg Leu Thr Thr Pro Glu Thr Ile Arg Ala Val
85 90 95gag tgg ctc aag tcc acc gtt gat gaa ggt ttc gca acc gtc tcc acc 336Glu Trp Leu Lys Ser Thr Val Asp Glu Gly Phe Ala Thr Val Ser Thr
100 105 110gac gtc acc aac gag ttc gca acc ggc ctg atc ggt tca tgc atc cag 384Asp Val Thr Asn Glu Phe Ala Thr Gly Leu Ile Gly Ser Cys Ile Gln
115 120 125tcc acc ggt gat ctg tct tcg gtt gcc ggc gct gca agc ttc gac tgg 432Ser Thr Gly Asp Leu Ser Ser Val Ala Gly Ala Ala Ser Phe Asp Trp
130 135 140ggc gta gca gca ctt cct aac cca acc ggc gag ggc gct tgc cca acc 480Gly Val Ala Ala Leu Pro Asn Pro Thr Gly Glu Gly Ala Cys Pro Thr145 150 155 160ggt ggc gca ggc ctg gga atc cca tct ggc atc tct gag cag cgt cag 528Gly Gly Ala Gly Leu Gly Ile Pro Ser Gly Ile Ser Glu Gln Arg Gln
165 170 175gac aac gcc ctg aag ttc atc gac ttc ctc acc aac gcc gcg aac act 576Asp Asn Ala Leu Lys Phe Ile Asp Phe Leu Thr Asn Ala Ala Asn Thr
180 185 190ggc tac tgg tcc cgc gag acc ggt tat gtt cca gtt cgt aag gat gct 624Gly Tyr Trp Ser Arg Glu Thr Gly Tyr Val Pro Val Arg Lys Asp Ala
195 200 205gca tct gat cca gat cac gca gca ttc ctc gag gag aac cct gca tac 672Ala Ser Asp Pro Asp His Ala Ala Phe Leu Glu Glu Asn Pro Ala Tyr
210 215 220aac gtt gca gtg gag cag ctt cct gat acc cgt tcc cag gac aac ttc 720Asn Val Ala Val Glu Gln Leu Pro Asp Thr Arg Ser Gln Asp Asn Phe225 230 235 240cgc gtg ctg ctg cca aac ggt gac cgc acc atc ggt gac gca ctg gag 768Arg Val Leu Leu Pro Asn Gly Asp Arg Thr Ile Gly Asp Ala Leu Glu
245 250 255aag atc tgc ctg act ggt gca gac atc gat gtc acc ctg gct gag gtt 816Lys Ile Cys Leu Thr Gly Ala Asp Ile Asp Val Thr Leu Ala Glu Val
260 265 270gag acc aag ctg aac acc atc tac acc cgc gac atc gaa cca ctt att 864Glu Thr Lys Leu Asn Thr Ile Tyr Thr Arg Asp Ile Glu Pro Leu Ile
275 280 285taatccgagc acttcagcta cac 887<210>198<211>288<212>PRT<213>谷氨酸棒杆菌<400>198Gly Gly His Tyr Gly Leu Pro Phe Ala Arg Ser Thr Val Leu Phe Tyr1 5 10 15Tyr Asn Lys Asp Leu Trp Ala Lys Ala Gly Leu Glu Asp Arg Gly Pro
20 25 30Glu Ser Trp Glu Glu Phe Ser Glu Trp Gly Pro Lys Leu Gln Glu Ala
35 40 45Met Asp Ser Gly Phe Ala His Gly Trp Gly Asp Ala Thr Asn Tyr Leu
50 55 60Ser Trp Thr Phe Glu Gly Pro Met Trp Ser Leu Gly Gly Asn Tyr Ser65 70 75 80Glu Gly Trp Glu Ser Arg Leu Thr Thr Pro Glu Thr Ile Arg Ala Val
85 90 95Glu Trp Leu Lys Ser Thr Val Asp Glu Gly Phe Ala Thr Val Ser Thr
100 105 110Asp Val Thr Asn Glu Phe Ala Thr Gly Leu Ile Gly Ser Cys Ile Gln
115 120 125Ser Thr Gly Asp Leu Ser Ser Val Ala Gly Ala Ala Ser Phe Asp Trp
130 135 140Gly Val Ala Ala Leu Pro Asn Pro Thr Gly Glu Gly Ala Cys Pro Thr145 150 155 160Gly Gly Ala Gly Leu Gly Ile Pro Ser Gly Ile Ser Glu Gln Arg Gln
165 170 175Asp Asn Ala Leu Lys Phe Ile Asp Phe Leu Thr Asn Ala Ala Asn Thr
180 185 190Gly Tyr Trp Ser Arg Glu Thr Gly Tyr Val Pro Val Arg Lys Asp Ala
195 200 205Ala Ser Asp Pro Asp His Ala Ala Phe Leu Glu Glu Asn Pro Ala Tyr
210 215 220Asn Val Ala Val Glu Gln Leu Pro Asp Thr Arg Ser Gln Asp Asn Phe225 230 235 240Arg Val Leu Leu Pro Asn Gly Asp Arg Thr Ile Gly Asp Ala Leu Glu
245 250 255Lys Ile Cys Leu Thr Gly Ala Asp Ile Asp Val Thr Leu Ala Glu Val
260 265 270Glu Thr Lys Leu Asn Thr Ile Tyr Thr Arg Asp Ile Glu Pro Leu Ile
275 280 285<210>199<211>842<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(819)<223>FRXA01891<400>199tac tac aac aag gat ctg tgg gct aag gct ggc ctg gaa gat cgt ggc 48Tyr Tyr Asn Lys Asp Leu Trp Ala Lys Ala Gly Leu Glu Asp Arg Gly1 5 10 15cca gag tca tgg gaa gag ttc tcc gag tgg ggt cca aag ctg cag gaa 96Pro Glu Ser Trp Glu Glu Phe Ser Glu Trp Gly Pro Lys Leu Gln Glu
20 25 30gcg atg gac agt ggt ttc gca cac ggt tgg gga gat gca acc aac tac 144Ala Met Asp Ser Gly Phe Ala His Gly Trp Gly Asp Ala Thr Asn Tyr
35 40 45ctt tct tgg act ttc gaa ggc cca atg tgg tcc ctc ggc ggc aac tac 192Leu Ser Trp Thr Phe Glu Gly Pro Met Trp Ser Leu Gly Gly Asn Tyr
50 55 60tct gaa ggt tgg gag tcc cgt ctg act acc cca gag acc atc cgt gca 240Ser Glu Gly Trp Glu Ser Arg Leu Thr Thr Pro Glu Thr Ile Arg Ala65 70 75 80gtt gag tgg ctc aag tcc acc gtt gat gaa ggt ttc gca acc gtc tcc 288Val Glu Trp Leu Lys Ser Thr Val Asp Glu Gly Phe Ala Thr Val Ser
85 90 95acc gac gtc acc aac gag ttc gca acc ggc ctg atc ggt tca tgc atc 336Thr Asp Val Thr Asn Glu Phe Ala Thr Gly Leu Ile Gly Ser Cys Ile
100 105 110cag tcc acc ggt gat ctg tct tcg gtt gcc ggc gct gca agc ttc gac 384Gln Ser Thr Gly Asp Leu Ser Ser Val Ala Gly Ala Ala Ser Phe Asp
115 120 125tgg ggc gta gca gca ctt cct aac cca acc ggc gag ggc gct tgc cca 432Trp Gly Val Ala Ala Leu Pro Asn Pro Thr Gly Glu Gly Ala Cys Pro
130 135 140acc ggt ggc gca ggc ctg gga atc cca tct ggc atc tct gag cag cgt 480Thr Gly Gly Ala Gly Leu Gly Ile Pro Ser Gly Ile Ser Glu Gln Arg145 150 155 160cag gac aac gcc ctg aag ttc atc gac ttc ctc acc aac gcc gcg aac 528Gln Asp Asn Ala Leu Lys Phe Ile Asp Phe Leu Thr Asn Ala Ala Asn
165 170 175act ggc tac tgg tcc cgc gag acc ggt tat gtt cca gtt cgt aag gat 576Thr Gly Tyr Trp Ser Arg Glu Thr Gly Tyr Val Pro Val Arg Lys Asp
180 185 190gct gca tct gat cca gat cac gca gca ttc ctc gag gag aac cct gca 624Ala Ala Ser Asp Pro Asp His Ala Ala Phe Leu Glu Glu Asn Pro Ala
195 200 205tac aac gtt gca gtg gag cag ctt cct gat acc cgt tcc cag gac aac 672Tyr Asn Val Ala Val Glu Gln Leu Pro Asp Thr Arg Ser Gln Asp Asn
210 215 220ttc cgc gtg ctg ctg cca aac ggt gac cgc acc atc ggt gac gca ctg 720Phe Arg Val Leu Leu Pro Asn Gly Asp Arg Thr Ile Gly Asp Ala Leu225 230 235 240gag aag atc tgc ctg act ggt gca gac atc gat gtc acc ctg gct gag 768Glu Lys Ile Cys Leu Thr Gly Ala Asp Ile Asp Val Thr Leu Ala Glu
245 250 255gtt gag acc aag ctg aac acc atc tac acc cgc gac atc gaa cca ctt 816Val Glu Thr Lys Leu Asn Thr Ile Tyr Thr Arg Asp Ile Glu Pro Leu
260 265 270att taatccgagc acttcagcta cac 842Ile<210>200<211>273<212>PRT<213>谷氨酸棒杆菌<400>200Tyr Tyr Asn Lys Asp Leu Trp Ala Lys Ala Gly Leu Glu Asp Arg Gly1 5 10 15Pro Glu Ser Trp Glu Glu Phe Ser Glu Trp Gly Pro Lys Leu Gln Glu
20 25 30Ala Met Asp Ser Gly Phe Ala His Gly Trp Gly Asp Ala Thr Asn Tyr
35 40 45Leu Ser Trp Thr Phe Glu Gly Pro Met Trp Ser Leu Gly Gly Asn Tyr
50 55 60Ser Glu Gly Trp Glu Ser Arg Leu Thr Thr Pro Glu Thr Ile Arg Ala65 70 75 80Val Glu Trp Leu Lys Ser Thr Val Asp Glu Gly Phe Ala Thr Val Ser
85 90 95Thr Asp Val Thr Asn Glu Phe Ala Thr Gly Leu Ile Gly Ser Cys Ile
100 105 110Gln Ser Thr Gly Asp Leu Ser Ser Val Ala Gly Ala Ala Ser Phe Asp
115 120 125Trp Gly Val Ala Ala Leu Pro Asn Pro Thr Gly Glu Gly Ala Cys Pro
130 135 140Thr Gly Gly Ala Gly Leu Gly Ile Pro Ser Gly Ile Ser Glu Gln Arg145 150 155 160Gln Asp Asn Ala Leu Lys Phe Ile Asp Phe Leu Thr Asn Ala Ala Asn
165 170 175Thr Gly Tyr Trp Ser Arg Glu Thr Gly Tyr Val Pro Val Arg Lys Asp
180 185 190Ala Ala Ser Asp Pro Asp His Ala Ala Phe Leu Glu Glu Asn Pro Ala
195 200 205Tyr Asn Val Ala Val Glu Gln Leu Pro Asp Thr Arg Ser Gln Asp Asn
210 215 220Phe Arg Val Leu Leu Pro Asn Gly Asp Arg Thr Ile Gly Asp Ala Leu225 230 235 240Glu Lys Ile Cys Leu Thr Gly Ala Asp Ile Asp Val Thr Leu Ala Glu
245 250 255Val Glu Thr Lys Leu Asn Thr Ile Tyr Thr Arg Asp Ile Glu Pro Leu
260 265 270Ile<210>201<211>776<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(7)..(753)<223>RXA02414<400>20ltttacc atg agt tac aac agc ccg tat aac aac acg aat ttc agc acc act 51
Met Ser Tyr Asn Ser Pro Tyr Asn Asn Thr Asn Phe Ser Thr Thr
1 5 10 15ggc gcg ttc caa cct gct ggt ggg ccg gtg aag ccg tgg aat aag ccc 99Gly Ala Phe Gln Pro Ala Gly Gly Pro Val Lys Pro Trp Asn Lys Pro
20 25 30gat gcc agc ctg aat cag cag ctg aaa aac aaa tcc cgt gtg cgc aca 147Asp Ala Ser Leu Asn Gln Gln Leu Lys Asn Lys Ser Arg Val Arg Thr
35 40 45ggt ctt acc atc gcc atc ggt tat gta gtg gtg att tgg gcg gtg cat 195Gly Leu Thr Ile Ala Ile Gly Tyr Val Val Val Ile Trp Ala Val His
50 55 60ttg gca tcc atc gtc att gcg ctg ctc act ggc ttc aac ctg acc aac 243Leu Ala Ser Ile Val Ile Ala Leu Leu Thr Gly Phe Asn Leu Thr Asn
65 70 75ttt ggt att cat ccg ctg gat acc agt gca ctg tgg ggt att ttc acc 291Phe Gly Ile His Pro Leu Asp Thr Ser Ala Leu Trp Gly Ile Phe Thr80 85 90 95tca ccg ctg ttg cat gga agc ttc agc cac ctc att gga aat acc gtt 339Ser Pro Leu Leu His Gly Ser Phe Ser His Leu Ile Gly Asn Thr Val
100 105 110cca ggc ttt ata ttc agt ttc ctc atc ggt atg agt ggc aag cgc gtg 387Pro Gly Phe Ile Phe Ser Phe Leu Ile Gly Met Ser Gly Lys Arg Val
115 120 125ttc tgg gaa gtc acg att atc gca ggt ctc atc ggc ggt ctt ggt aca 435Phe Trp Glu Val Thr Ile Ile Ala Gly Leu Ile Gly Gly Leu Gly Thr
130 135 140tgg att ttc ggt gga atc ggc acc aac cac atc ggt gcg tcc ggc ctg 483Trp Ile Phe Gly Gly Ile Gly Thr Asn His Ile Gly Ala Ser Gly Leu
145 150 155att tat ggc tgg ctt ggc tac ctg atc gtg cgt gga att ttc aac aag 531Ile Tyr Gly Trp Leu Gly Tyr Leu Ile Val Arg Gly Ile Phe Asn Lys160 165 170 175gat att aaa cag ttc ctg ctt ggc ata gtt ttg gcg ttc att tac tcc 579Asp Ile Lys Gln Phe Leu Leu Gly Ile Val Leu Ala Phe Ile Tyr Ser
180 185 190ggt ttg ttc tgg ggt ctg cta cct act caa atc ggt gtg tcc tgg cag 627Gly Leu Phe Trp Gly Leu Leu Pro Thr Gln Ile Gly Val Ser Trp Gln
195 200 205ggc cac ctt ttc ggt gca ctt ggt gga atc ggt gcg ggt gct ttt atc 675Gly His Leu Phe Gly Ala Leu Gly Gly Ile Gly Ala Gly Ala Phe Ile
210 215 220gcc tcg gat gac ccg gca gcg ttg aaa gcg aag aag caa cag aag aaa 723Ala Ser Asp Asp Pro Ala Ala Leu Lys Ala Lys Lys Gln Gln Lys Lys
225 230 235tta gaa aag caa caa cgc caa aga ggc tta tagttttcac ctagcgacta 773Leu Glu Lys Gln Gln Arg Gln Arg Gly Leu240 245cac 776<210>202<211>249<212>PRT<213>谷氨酸棒杆菌<400>202Met Ser Tyr Asn Ser Pro Tyr Asn Asn Thr Asn Phe Ser Thr Thr Gly1 5 10 15Ala Phe Gln Pro Ala Gly Gly Pro Val Lys Pro Trp Asn Lys Pro Asp
20 25 30Ala Ser Leu Asn Gln Gln Leu Lys Asn Lys Ser Arg Val Arg Thr Gly
35 40 45Leu Thr Ile Ala Ile Gly Tyr Val Val Val Ile Trp Ala Val His Leu
50 55 60Ala Ser Ile Val Ile Ala Leu Leu Thr Gly Phe Asn Leu Thr Asn Phe65 70 75 80Gly Ile His Pro Leu Asp Thr Ser Ala Leu Trp Gly Ile Phe Thr Ser
85 90 95Pro Leu Leu His Gly Ser Phe Ser His Leu Ile Gly Asn Thr Val Pro
100 105 110Gly Phe Ile Phe Ser Phe Leu Ile Gly Met Ser Gly Lys Arg Val Phe
115 120 125Trp Glu Val Thr Ile Ile Ala Gly Leu Ile Gly Gly Leu Gly Thr Trp
130 135 140Ile Phe Gly Gly Ile Gly Thr Asn His Ile Gly Ala Ser Gly Leu Ile145 150 155 160Tyr Gly Trp Leu Gly Tyr Leu Ile Val Arg Gly Ile Phe Asn Lys Asp
165 170 175Ile Lys Gln Phe Leu Leu Gly Ile Val Leu Ala Phe Ile Tyr Ser Gly
180 185 190Leu Phe Trp Gly Leu Leu Pro Thr Gln Ile Gly Val Ser Trp Gln Gly
195 200 205His Leu Phe Gly Ala Leu Gly Gly Ile Gly Ala Gly Ala Phe Ile Ala
210 215 220Ser Asp Asp Pro Ala Ala Leu Lys Ala Lys Lys Gln Gln Lys Lys Leu225 230 235 240Glu Lys Gln Gln Arg Gln Arg Gly Leu
245<210>203<211>840<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(817)<223>RXN01580<400>203cggtaaacgc ctcattaaag tccaatgcca tgctcataac actaacagtt aaccgtgcgg 60tcaactttgc tccctatcct taaaaagccc acagaaaagg atg tat aag aat atg 115
Met Tyr Lys Asn Met
1 5cac att gtt gcc cat cgc ggt gcg gaa gat ctg cac ctc gaa aac acc 163His Ile Val Ala His Arg Gly Ala Glu Asp Leu His Leu Glu Asn Thr
10 15 20atg acc gct ttc cag gct gcc gcg ccc gct gac gct ttt gag ctg gat 211Met Thr Ala Phe Gln Ala Ala Ala Pro Ala Asp Ala Phe Glu Leu Asp
25 30 35atc cac gcc acc gct gac aat cag gtc gtc gtt atc cac gac cgc acc 259Ile His Ala Thr Ala Asp Asn Gln Val Val Val Ile His Asp Arg Thr
40 45 50gca gcg cgt gtt gcc gcg cca gat tcc ctg cac cgc gac acc ccg gtt 307Ala Ala Arg Val Ala Ala Pro Asp Ser Leu His Arg Asp Thr Pro Val
55 60 65gcg cgc tta agc gcc gcg caa atc aag gag ata acg ctt atc gac gga 355Ala Arg Leu Ser Ala Ala Gln Ile Lys Glu Ile Thr Leu Ile Asp Gly70 75 80 85tcc ccc gta cca acc ctg gag gaa gtt cta ctc cag acg agc ctg ccg 403Ser Pro Val Pro Thr Leu Glu Glu Val Leu Leu Gln Thr Ser Leu Pro
90 95 100atc caa gtg gaa atc aaa tct gcc ggt gca gtt cca gca gcc gca gca 451Ile Gln Val Glu Ile Lys Ser Ala Gly Ala Val Pro Ala Ala Ala Ala
105 110 115tta ttg cag aaa tac cca gag cac ctg gag cgc ctg ctg ttc atc agt 499Leu Leu Gln Lys Tyr Pro Glu His Leu Glu Arg Leu Leu Phe Ile Ser
120 125 130ttc atc gat gca gca ctg gtg gaa atc gtg gat cga ctg cca gaa gct 547Phe Ile Asp Ala Ala Leu Val Glu Ile Val Asp Arg Leu Pro Glu Ala
135 140 145cgc gtg gga atc ttg cgc gat gcg tcc atg gat gat ctg cgc att ctt 595Arg Val Gly Ile Leu Arg Asp Ala Ser Met Asp Asp Leu Arg Ile Leu150 155 160 165gat tac atc ccg cta aaa aat gtg ggc gcg atc ttg ccc tcg tgg aaa 643Asp Tyr Ile Pro Leu Lys Asn Val Gly Ala Ile Leu Pro Ser Trp Lys
170 175 180gca cta aac gtg gcg tca att gct gat cta cat acc aag gga atc aag 691Ala Leu Asn Val Ala Ser Ile Ala Asp Leu His Thr Lys Gly Ile Lys
185 190 195gtt ggc tgc tgg aca att cgg gat gaa aat gcg ttt ggg atc gca caa 739Val Gly Cys Trp Thr Ile Arg Asp Glu Asn Ala Phe Gly Ile Ala Gln
200 205 210caa gct ggc gtt gat tac gcc act gtt agc gat ccc tct cgt ttc ctc 787Gln Ala Gly Val Asp Tyr Ala Thr Val Ser Asp Pro Ser Arg Phe Leu
215 220 225gcg ccc tcc cct gct ggg gag ttg cac tgg taaataatct agtgaccaga 837Ala Pro Ser Pro Ala Gly Glu Leu His Trp230 235ctg 840<210>204<211>239<212>PRT<213>谷氨酸棒杆菌<400>204Met Tyr Lys Asn Met His Ile Val Ala His Arg Gly Ala Glu Asp Leu1 5 10 15His Leu Glu Asn Thr Met Thr Ala Phe Gln Ala Ala Ala Pro Ala Asp
20 25 30Ala Phe Glu Leu Asp Ile His Ala Thr Ala Asp Asn Gln Val Val Val
35 40 45Ile His Asp Arg Thr Ala Ala Arg Val Ala Ala Pro Asp Ser Leu His
50 55 60Arg Asp Thr Pro Val Ala Arg Leu Ser Ala Ala Gln Ile Lys Glu Ile65 70 75 80Thr Leu Ile Asp Gly Ser Pro Val Pro Thr Leu Glu Glu Val Leu Leu
85 90 95Gln Thr Ser Leu Pro Ile Gln Val Glu Ile Lys Ser Ala Gly Ala Val
100 105 110Pro Ala Ala Ala Ala Leu Leu Gln Lys Tyr Pro Glu His Leu Glu Arg
115 120 125Leu Leu Phe Ile Ser Phe Ile Asp Ala Ala Leu Val Glu Ile Val Asp
130 135 140Arg Leu Pro Glu Ala Arg Val Gly Ile Leu Arg Asp Ala Ser Met Asp145 150 155 160Asp Leu Arg Ile Leu Asp Tyr Ile Pro Leu Lys Asn Val Gly Ala Ile
165 170 175Leu Pro Ser Trp Lys Ala Leu Asn Val Ala Ser Ile Ala Asp Leu His
180 185 190Thr Lys Gly Ile Lys Val Gly Cys Trp Thr Ile Arg Asp Glu Asn Ala
195 200 205Phe Gly Ile Ala Gln Gln Ala Gly Val Asp Tyr Ala Thr Val Ser Asp
210 215 220Pro Ser Arg Phe Leu Ala Pro Ser Pro Ala Gly Glu Leu His Trp225 230 235<210>205<211>1314<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1291)<223>RXA01436<400>205gcctaaacaa accagtcaac gacctttccc gtggcgcaac agtccctgac atcgtcaaca 60cagtagccat cacagcaatt caggcaggag gacgcagcta atg gca ttg gca ctt 115
Met Ala Leu Ala Leu
1 5gtt ttg aac tcc ggt tca tct tcc atc aaa ttc cag ctg gtc aac ccc 163Val Leu Asn Ser Gly Ser Ser Ser Ile Lys Phe Gln Leu Val Asn Pro
10 15 20gaa aac tct gcc atc gac gag cca tat gtt tct ggt ctt gtg gag cag 211Glu Asn Ser Ala Ile Asp Glu Pro Tyr Val Ser Gly Leu Val Glu Gln
25 30 35att ggt gag cca aac ggc cgc atc gta ctc aaa ata gag ggt gaa aaa 259Ile Gly Glu Pro Asn Gly Arg Ile Val Leu Lys Ile Glu Gly Glu Lys
40 45 50tat acc cta gag aca ccc atc gca gat cac tcc gaa ggc cta aac ctg 307Tyr Thr Leu Glu Thr Pro Ile Ala Asp His Ser Glu Gly Leu Asn Leu
55 60 65gcg ttc gat ctc atg gac cag cac aac tgt ggt cct tcc caa ctg gaa 355Ala Phe Asp Leu Met Asp Gln His Asn Cys Gly Pro Ser Gln Leu Glu70 75 80 85atc acc gca gtt gga cac cgc gtg gtc cac ggc gga atc ttg ttc tcc 403Ile Thr Ala Val Gly His Arg Val Val His Gly Gly Ile Leu Phe Ser
90 95 100gca ccg gaa ctt atc act gat gaa atc gtg gaa atg atc cgc gat ctc 451Ala Pro Glu Leu Ile Thr Asp Glu Ile Val Glu Met Ile Arg Asp Leu
105 110 115att cca ctc gca cca ctg cac aac cct gca aac gtt gac ggc att gat 499Ile Pro Leu Ala Pro Leu His Asn Pro Ala Asn Val Asp Gly Ile Asp
120 125 130gtt gct cga aaa att ctc ccc gat gtc cca cac gta gct gtc ttt gac 547Val Ala Arg Lys Ile Leu Pro Asp Val Pro His Val Ala Val Phe Asp
135 140 145acc ggt ttc ttc cac tca ctt cca cca gca gct gcg ctg tat gcc atc 595Thr Gly Phe Phe His Ser Leu Pro Pro Ala Ala Ala Leu Tyr Ala Ile150 155 160 165aac aag gat gtc gca gct gaa cac gga atc agg cgc tat ggt ttc cac 643Asn Lys Asp Val Ala Ala Glu His Gly Ile Arg Arg Tyr Gly Phe His
170 175 180ggc acc tcc cat gaa ttt gtg tcc aag cgc gtg gtg gaa att ctg gaa 691Gly Thr Ser His Glu Phe Val Ser Lys Arg Val Val Glu Ile Leu Glu
185 190 195aag ccc acc gaa gac atc aac acc atc acc ttc cac ctg ggc aac ggc 739Lys Pro Thr Glu Asp Ile Asn Thr Ile Thr Phe His Leu Gly Asn Gly
200 205 210gca tcc atg gct gct gtt caa ggt ggc cgt gcg gta gat act tcc atg 787Ala Ser Met Ala Ala Val Gln Gly Gly Arg Ala Val Asp Thr Ser Met
215 220 225ggt atg aca cct ctc gcg ggc ctt gtc atg ggt acc cga agc ggt gac 835Gly Met Thr Pro Leu Ala Gly Leu Val Met Gly Thr Arg Ser Gly Asp230 235 240 245att gat cca ggt atc gtc ttc cac ctt tcc cgc acc gct ggc atg agc 883Ile Asp Pro Gly Ile Val Phe His Leu Ser Arg Thr Ala Gly Met Ser
250 255 260atc gat gag atc gat aat ctg ctg aac aaa aag tcg ggt gta aag gga 931Ile Asp Glu Ile Asp Asn Leu Leu Asn Lys Lys Ser Gly Val Lys Gly
265 270 275ctt tcc ggt gtt aat gat ttc cgt gaa ctg cgg gaa atg atc gac aac 979Leu Ser Gly Val Asn Asp Phe Arg Glu Leu Arg Glu Met Ile Asp Asn
280 285 290aat gat caa gat gcc tgg tcc gcg tac aac att tac ata cac caa ctc 1027Asn Asp Gln Asp Ala Trp Ser Ala Tyr Asn Ile Tyr Ile His Gln Leu
295 300 305cgc cgc tac ctc ggt tcc tac atg gtg gca ctg gga cgg gta gac acc 1075Arg Arg Tyr Leu Gly Ser Tyr Met Val Ala Leu Gly Arg Val Asp Thr310 315 320 325atc gtg ttc acc gcc ggt gtc ggt gaa aat gcc cag ttt gtc cgt gag 1123Ile Val Phe Thr Ala Gly Val Gly Glu Asn Ala Gln Phe Val Arg Glu
330 335 340gat gcc ttg gca ggt ttg gaa atg tac gga att gag atc gat cca gag 1171Asp Ala Leu Ala Gly Leu Glu Met Tyr Gly Ile Glu Ile Asp Pro Glu
345 350 355cgt aac gca ttg cca aac gat ggt cct cga ttg att tcc acc gat gcc 1219Arg Asn Ala Leu Pro Asn Asp Gly Pro Arg Leu Ile Ser Thr Asp Ala
360 365 370tcc aag gtg aag gtg ttt gtt att cca act aat gaa gag tta gct atc 1267Ser Lys Val Lys Val Phe Val Ile Pro Thr Asn Glu Glu Leu Ala Ile
375 380 385gct agg tac gcg gtg aag ttc gct tagctctcct ggttaggatc cac 1314Ala Arg Tyr Ala Val Lys Phe Ala390 395<210>206<211>397<212>PRT<213>谷氨酸棒杆菌<400>206Met Ala Leu Ala Leu Val Leu Asn Ser Gly Ser Ser Ser Ile Lys Phe1 5 10 15Gln Leu Val Asn Pro Glu Asn Ser Ala Ile Asp Glu Pro Tyr Val Ser
20 25 30Gly Leu Val Glu Gln Ile Gly Glu Pro Asn Gly Arg Ile Val Leu Lys
35 40 45Ile Glu Gly Glu Lys Tyr Thr Leu Glu Thr Pro Ile Ala Asp His Ser
50 55 60Glu Gly Leu Asn Leu Ala Phe Asp Leu Met Asp Gln His Asn Cys Gly65 70 75 80Pro Ser Gln Leu Glu Ile Thr Ala Val Gly His Arg Val Val His Gly
85 90 95Gly Ile Leu Phe Ser Ala Pro Glu Leu Ile Thr Asp Glu Ile Val Glu
100 105 110Met Ile Arg Asp Leu Ile Pro Leu Ala Pro Leu His Asn Pro Ala Asn
115 120 125Val Asp Gly Ile Asp Val Ala Arg Lys Ile Leu Pro Asp Val Pro His
130 135 140Val Ala Val Phe Asp Thr Gly Phe Phe His Ser Leu Pro Pro Ala Ala145 150 155 160Ala Leu Tyr Ala Ile Asn Lys Asp Val Ala Ala Glu His Gly Ile Arg
165 170 175Arg Tyr Gly Phe His Gly Thr Ser His Glu Phe Val Ser Lys Arg Val
180 185 190Val Glu Ile Leu Glu Lys Pro Thr Glu Asp Ile Asn Thr Ile Thr Phe
195 200 205His Leu Gly Asn Gly Ala Ser Met Ala Ala Val Gln Gly Gly Arg Ala
210 215 220Val Asp Thr Ser Met Gly Met Thr Pro Leu Ala Gly Leu Val Met Gly225 230 235 240Thr Arg Ser Gly Asp Ile Asp Pro Gly Ile Val Phe His Leu Ser Arg
245 250 255Thr Ala Gly Met Ser Ile Asp Glu Ile Asp Asn Leu Leu Asn Lys Lys
260 265 270Ser Gly Val Lys Gly Leu Ser Gly Val Asn Asp Phe Arg Glu Leu Arg
275 280 285Glu Met Ile Asp Asn Asn Asp Gln Asp Ala Trp Ser Ala Tyr Asn Ile
290 295 300Tyr Ile His Gln Leu Arg Arg Tyr Leu Gly Ser Tyr Met Val Ala Leu305 310 315 320Gly Arg Val Asp Thr Ile Val Phe Thr Ala Gly Val Gly Glu Asn Ala
325 330 335Gln Phe Val Arg Glu Asp Ala Leu Ala Gly Leu Glu Met Tyr Gly Ile
340 345 350Glu Ile Asp Pro Glu Arg Asn Ala Leu Pro Asn Asp Gly Pro Arg Leu
355 360 365Ile Ser Thr Asp Ala Ser Lys Val Lys Val Phe Val Ile Pro Thr Asn
370 375 380Glu Glu Leu Ala Ile Ala Arg Tyr Ala Val Lys Phe Ala385 390<210>207<211>927<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(904)<223>RXA00686<400>207ataggcttga acaatacgtc gttacactgg ccgatttgat acctttcaaa acttttaccc 60ttcatcggag tgccagggga acttagagga gcattaaata atg gcg gga gga aat 115
Met Ala Gly Gly Asn
1 5cgc gaa cct gga cgt aca gtc acc tcc aag gtg atc gcc gta ctg gga 163Arg Glu Pro Gly Arg Thr Val Thr Ser Lys Val Ile Ala Val Leu Gly
10 15 20gct ttt gaa cac acc atg cgt cca ctt ggt gtc act gaa atc gct gag 211Ala Phe Glu His Thr Met Arg Pro Leu Gly Val Thr Glu Ile Ala Glu
25 30 35ctg gca gac ctc cca cca agt acc acc cac cgt ctc gtt tct gaa tta 259Leu Ala Asp Leu Pro Pro Ser Thr Thr His Arg Leu Val Ser Glu Leu
40 45 50acc gaa ggc gga cta ctc agc aag aaa tct gat ggg cgc tac caa ttg 307Thr Glu Gly Gly Leu Leu Ser Lys Lys Ser Asp Gly Arg Tyr Gln Leu
55 60 65ggc tta cgt atc tgg gaa ctc gcc caa aat aca gga cgg cag tta cgc 355Gly Leu Arg Ile Trp Glu Leu Ala Gln Asn Thr Gly Arg Gln Leu Arg70 75 80 85gac act gca cgc ccg ttc atc caa gag ctc tac tca ctt act tcc gag 403Asp Thr Ala Arg Pro Phe Ile Gln Glu Leu Tyr Ser Leu Thr Ser Glu
90 95 100act gcg cag cta gtg gtc cgc gat aaa gat gaa gca ctt ttg att gac 451Thr Ala Gln Leu Val Val Arg Asp Lys Asp Glu Ala Leu Leu Ile Asp
105 110 115cga gcc tac ggc acg aag aaa att cca cgc tcg gct cga gtc ggt ggt 499Arg Ala Tyr Gly Thr Lys Lys Ile Pro Arg Ser Ala Arg Val Gly Gly
120 125 130cga cta cct ctg aac tcc act gcg gtt ggc aag att ctc ctt gcg ttt 547Arg Leu Pro Leu Asn Ser Thr Ala Val Gly Lys Ile Leu Leu Ala Phe
135 140 145gat gag cca tgg gta aaa cag tcc tat ctc aag ctg cca ctc aac gcc 595Asp Glu Pro Trp Val Lys Gln Ser Tyr Leu Lys Leu Pro Leu Asn Ala150 155 160 165tcc acc cca aag aca att gtg aat ccc gac gtc ttg gct gcg cag ctg 643Ser Thr Pro Lys Thr Ile Val Asn Pro Asp Val Leu Ala Ala Gln Leu
170 175 180aaa caa att cac tcg caa ggc ttt gcc atc aca cat gac gag caa cga 691Lys Gln Ile His Ser Gln Gly Phe Ala Ile Thr His Asp Glu Gln Arg
185 190 195atc ggc ggc gca tcg atc gcc gta ccg gtc tgg cat aca gga aaa ctg 739Ile Gly Gly Ala Ser Ile Ala Val Pro Val Trp His Thr Gly Lys Leu
200 205 210gga gca gca ctg ggg ttg gtg gtt ccc acc gca cag gct gca aat ctt 787Gly Ala Ala Leu Gly Leu Val Val Pro Thr Ala Gln Ala Ala Asn Leu
215 220 225gag cgc tat ctc ccg atc ctt cag gcg aca agt cag aga att aca aaa 835Glu Arg Tyr Leu Pro Ile Leu Gln Ala Thr Ser Gln Arg Ile Thr Lys230 235 240 245gca acc gcg ctc att cct ttg gac aca ctt ttg gct tca cac aaa aat 883Ala Thr Ala Leu Ile Pro Leu Asp Thr Leu Leu Ala Ser His Lys Asn
250 255 260gca gaa cga aaa ggc gat acc taaacccgcc ctccatctgc ata 927Ala Glu Arg Lys Gly Asp Thr
265<210>208<211>268<212>PRT<213>谷氨酸棒杆菌<400>208Met Ala Gly Gly Asn Arg Glu Pro Gly Arg Thr Val Thr Ser Lys Val1 5 10 15Ile Ala Val Leu Gly Ala Phe Glu His Thr Met Arg Pro Leu Gly Val
20 25 30Thr Glu Ile Ala Glu Leu Ala Asp Leu Pro Pro Ser Thr Thr His Arg
35 40 45Leu Val Ser Glu Leu Thr Glu Gly Gly Leu Leu Ser Lys Lys Ser Asp
50 55 60Gly Arg Tyr Gln Leu Gly Leu Arg Ile Trp Glu Leu Ala Gln Asn Thr65 70 75 80Gly Arg Gln Leu Arg Asp Thr Ala Arg Pro Phe Ile Gln Glu Leu Tyr
85 90 95Ser Leu Thr Ser Glu Thr Ala Gln Leu Val Val Arg Asp Lys Asp Glu
100 105 110Ala Leu Leu Ile Asp Arg Ala Tyr Gly Thr Lys Lys Ile Pro Arg Ser
115 120 125Ala Arg Val Gly Gly Arg Leu Pro Leu Asn Ser Thr Ala Val Gly Lys
130 135 140Ile Leu Leu Ala Phe Asp Glu Pro Trp Val Lys Gln Ser Tyr Leu Lys145 150 155 160Leu Pro Leu Asn Ala Ser Thr Pro Lys Thr Ile Val Asn Pro Asp Val
165 170 175Leu Ala Ala Gln Leu Lys Gln Ile His Ser Gln Gly Phe Ala Ile Thr
180 185 190His Asp Glu Gln Arg Ile Gly Gly Ala Ser Ile Ala Val Pro Val Trp
195 200 205His Thr Gly Lys Leu Gly Ala Ala Leu Gly Leu Val Val Pro Thr Ala
210 215 220Gln Ala Ala Asn Leu Glu Arg Tyr Leu Pro Ile Leu Gln Ala Thr Ser225 230 235 240Gln Arg Ile Thr Lys Ala Thr Ala Leu Ile Pro Leu Asp Thr Leu Leu
245 250 255Ala Ser His Lys Asn Ala Glu Arg Lys Gly Asp Thr
260 265<210>209<211>1158<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1135)<223>RXA00246<400>209ttgcaaggat tgtaatttaa ggcacatcta tgtcggtgtg aaattacatg tgccagaaga 60gcaatttgcc aagtaatcca agcgagaagg agtgagtttt atg acc act gct gca 115
Met Thr Thr Ala Ala
1 5ccc caa gaa ttt acc gct gct gtt gtt gaa aaa ttc ggt cat gac gtg 163Pro Gln Glu Phe Thr Ala Ala Val Val Glu Lys Phe Gly His Asp Val
10 15 20acc gtg aag gat att gac ctt cca aag cca ggg cca cac cag gca ttg 211Thr Val Lys Asp Ile Asp Leu Pro Lys Pro Gly Pro His Gln Ala Leu
25 30 35gtg aag gta ctc acc tcc ggc atc tgc cac acc gac ctc cac gcc ttg 259Val Lys Val Leu Thr Ser Gly Ile Cys His Thr Asp Leu His Ala Leu
40 45 50gag ggc gat tgg cca gta aag ccg gaa cca cca ttc gta cca gga cac 307Glu Gly Asp Trp Pro Val Lys Pro Glu Pro Pro Phe Val Pro Gly His
55 60 65gaa ggt gta ggt gaa gtt gtt gag ctc gga cca ggt gaa cac gat gtg 355Glu Gly Val Gly Glu Val Val Glu Leu Gly Pro Gly Glu His Asp Val70 75 80 85aag gtc ggc gat att gtc ggc aat gcg tgg ctc tgg tca gcg tgt ggc 403Lys Val Gly Asp Ile Val Gly Asn Ala Trp Leu Trp Ser Ala Cys Gly
90 95 100acc tgc gaa tac tgc atc acc ggc agg gaa act cag tgc aac gaa gct 451Thr Cys Glu Tyr Cys Ile Thr Gly Arg Glu Thr Gln Cys Asn Glu Ala
105 110 115gag tat ggt ggc tac acc caa aat gga tcc ttc ggc cag tac atg ctg 499Glu Tyr Gly Gly Tyr Thr Gln Asn Gly Ser Phe Gly Gln Tyr Met Leu
120 125 130gtg gat acc cgt tac gcc gct cgc atc cca gac ggc gtg gac tac ctc 547Val Asp Thr Arg Tyr Ala Ala Arg Ile Pro Asp Gly Val Asp Tyr Leu
135 140 145gaa gca gca cca att ctg tgt gca ggc gtg act gtc tac aag gca ctc 595Glu Ala Ala Pro Ile Leu Cys Ala Gly Val Thr Val Tyr Lys Ala Leu150 155 160 165aaa gtc tct gaa acc cgc ccg ggc caa ttc atg gtg atc tcc ggt gtc 643Lys Val Ser Glu Thr Arg Pro Gly Gln Phe Met Val Ile Ser Gly Val
170 175 180ggc gga ctt ggc cac atc gca gtc caa tac gca gcg gcg atg ggc atg 691Gly Gly Leu Gly His Ile Ala Val Gln Tyr Ala Ala Ala Met Gly Met
185 190 195cgt gtc att gcg gta gat att gcc gat gac aag ctg gaa ctt gcc cgt 739Arg Val Ile Ala Val Asp Ile Ala Asp Asp Lys Leu Glu Leu Ala Arg
200 205 210aag cac ggt gcg gaa ttt acc gtg aat gcg cgt aat gaa gat tca ggc 787Lys His Gly Ala Glu Phe Thr Val Asn Ala Arg Asn Glu Asp Ser Gly
215 220 225gaa gct gta cag aag tac acc aac ggt ggc gca cac ggc gtg ctt gtg 835Glu Ala Val Gln Lys Tyr Thr Asn Gly Gly Ala His Gly Val Leu Val230 235 240 245act gca gtt cac gag gca gca ttc ggc cag gca ctg gat atg gct cga 883Thr Ala Val His Glu Ala Ala Phe Gly Gln Ala Leu Asp Met Ala Arg
250 255 260cgt gca gga aca att gtg ttc aac ggt ctg cca ccg gga gag ttc cca 931Arg Ala Gly Thr Ile Val Phe Asn Gly Leu Pro Pro Gly Glu Phe Pro
265 270 275gca tcc gtg ttc aac atc gta ttc aag ggc ctg acc atc cgt gga tcc 979Ala Ser Val Phe Asn Ile Val Phe Lys Gly Leu Thr Ile Arg Gly Ser
280 285 290ctc gtg gga acc cgc caa gac ttg gcc gaa gcg ctc gat ttc ttt gca 1027Leu Val Gly Thr Arg Gln Asp Leu Ala Glu Ala Leu Asp Phe Phe Ala
295 300 305cgc gga cta atc aag cca acc gtg agt gag tgc tcc ctc gat gag gtc 1075Arg Gly Leu Ile Lys Pro Thr Val Ser Glu Cys Ser Leu Asp Glu Val310 315 320 325aat ggt gtg ctt gac cgc atg cga aac ggc aag atc gat ggt cgt gtg 1123Asn Gly Val Leu Asp Arg Met Arg Asn Gly Lys Ile Asp Gly Arg Val
330 335 340gcg att cgt ttc taacggattg tgttgaaact gct 1158Ala Ile Arg Phe
345<210>210<211>345<212>PRT<2 13>谷氨酸棒杆菌<400>210Met Thr Thr Ala Ala Pro Gln Glu Phe Thr Ala Ala Val Val Glu Lys1 5 10 15Phe Gly His Asp Val Thr Val Lys Asp Ile Asp Leu Pro Lys Pro Gly
20 25 30Pro His Gln Ala Leu Val Lys Val Leu Thr Ser Gly Ile Cys His Thr
35 40 45Asp Leu His Ala Leu Glu Gly Asp Trp Pro Val Lys Pro Glu Pro Pro
50 55 60Phe Val Pro Gly His Glu Gly Val Gly Glu Val Val Glu Leu Gly Pro65 70 75 80Gly Glu His Asp Val Lys Val Gly Asp Ile Val Gly Asn Ala Trp Leu
85 90 95Trp Ser Ala Cys Gly Thr Cys Glu Tyr Cys Ile Thr Gly Arg Glu Thr
100 105 110Gln Cys Asn Glu Ala Glu Tyr Gly Gly Tyr Thr Gln Asn Gly Ser Phe
115 120 125Gly Gln Tyr Met Leu Val Asp Thr Arg Tyr Ala Ala Arg Ile Pro Asp
130 135 140Gly Val Asp Tyr Leu Glu Ala Ala Pro Ile Leu Cys Ala Gly Val Thr145 150 155 160Val Tyr Lys Ala Leu Lys Val Ser Glu Thr Arg Pro Gly Gln Phe Met
165 170 175Val Ile Ser Gly Val Gly Gly Leu Gly His Ile Ala Val Gln Tyr Ala
180 185 190Ala Ala Met Gly Met Arg Val Ile Ala Val Asp Ile Ala Asp Asp Lys
195 200 205Leu Glu Leu Ala Arg Lys His Gly Ala Glu Phe Thr Val Asn Ala Arg
210 215 220Asn Glu Asp Ser Gly Glu Ala Val Gln Lys Tyr Thr Asn Gly Gly Ala225 230 235 240His Gly Val Leu Val Thr Ala Val His Glu Ala Ala Phe Gly Gln Ala
245 250 255Leu Asp Met Ala Arg Arg Ala Gly Thr Ile Val Phe Asn Gly Leu Pro
260 265 270Pro Gly Glu Phe Pro Ala Ser Val Phe Asn Ile Val Phe Lys Gly Leu
275 280 285Thr Ile Arg Gly Ser Leu Val Gly Thr Arg Gln Asp Leu Ala Glu Ala
290 295 300Leu Asp Phe Phe Ala Arg Gly Leu Ile Lys Pro Thr Val Ser Glu Cys305 310 315 320Ser Leu Asp Glu Val Asn Gly Val Leu Asp Arg Met Arg Asn Gly Lys
325 330 335Ile Asp Gly Arg Val Ala Ile Arg Phe
340 345<210>211<211>723<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(700)<223>RXA01571<400>211aaactacctg ctgagagctt tgtaatttac ggtgtggttg tggaggggtg cgtcgagaag 60cgctcgtagg cgcttttgat ttttcggtag gctaactggg gtg agt atc tca gta 115
Val Ser Ile Ser Val
1 5aaa gca cta caa aag tcc ggc cca gaa gca cct ttc gag gtc aag atc 163Lys Ala Leu Gln Lys Ser Gly Pro Glu Ala Pro Phe Glu Val Lys Ile
10 15 20att gaa cgc cgt gac cca cgc gca gat gat gtg gtt att gat atc aaa 211Ile Glu Arg Arg Asp Pro Arg Ala Asp Asp Val Val Ile Asp Ile Lys
25 30 35gct gcg ggc atc tgc cac agc gat atc cac acc atc cgc aac gaa tgg 259Ala Ala Gly Ile Cys His Ser Asp Ile His Thr Ile Arg Asn Glu Trp
40 45 50ggc gag gcg cac ttc ccg ctc acc gtc ggc cac gaa atc gca ggc gtt 307Gly Glu Ala His Phe Pro Leu Thr Val Gly His Glu Ile Ala Gly Val
55 60 65gtc tct gcg gtt gga tcc gat gta acc aaa tgg aaa gtc ggc gac cgc 355Val Ser Ala Val Gly Ser Asp Val Thr Lys Trp Lys Val Gly Asp Arg70 75 80 85gtg ggc gtc ggc tgc ctc gtt aac tcc tgc ggc gaa tgc gaa cag tgc 403Val Gly Val Gly Cys Leu Val Asn Ser Cys Gly Glu Cys Glu Gln Cys
90 95 100gtc gca gga ttt gaa aac aac tgc ctt cgc gga aac gtc gga acc tac 451Val Ala Gly Phe Glu Asn Asn Cys Leu Arg Gly Asn Val Gly Thr Tyr
105 110 115aac tct aac gac gtc gac ggc acc atc acc caa ggc ggc tac gct gaa 499Asn Ser Asn Asp Val Asp Gly Thr Ile Thr Gln Gly Gly Tyr Ala Glu
120 125 130aag gta gtg gtc aac gaa cgt ttc ctg tgc agc atc cca gag gaa ctt 547Lys Val Val Val Asn Glu Arg Phe Leu Cys Ser Ile Pro Glu Glu Leu
135 140 145aac ttc gat gtc gca gca cca ctg ctg tgc gca ggc atc acc acc tac 595Asn Phe Asp Val Ala Ala Pro Leu Leu Cys Ala Gly Ile Thr Thr Tyr150 155 160 165tcc cca atc gct cgc tgg aac gtt aaa gaa ggc gac aaa gta gca gtc 643Ser Pro Ile Ala Arg Trp Asn Val Lys Glu Gly Asp Lys Val Ala Val
170 175 180atg ggc ctc ggc ggg act cgg aca cat ggg tgt cca gat cgc tgc agc 691Met Gly Leu Gly Gly Thr Arg Thr His Gly Cys Pro Asp Arg Cys Ser
185 190 195caa ggg tgc tgaggttacc gttctgtccc gtt 723Gln Gly Cys
200<210>212<211>200<212>PRT<213>谷氨酸棒杆菌<400>212Val Ser Ile Ser Val Lys Ala Leu Gln Lys Ser Gly Pro Glu Ala Pro1 5 10 15Phe Glu Val Lys Ile Ile Glu Arg Arg Asp Pro Arg Ala Asp Asp Val
20 25 30Val Ile Asp Ile Lys Ala Ala Gly Ile Cys His Ser Asp Ile His Thr
35 40 45Ile Arg Asn Glu Trp Gly Glu Ala His Phe Pro Leu Thr Val Gly His
50 55 60Glu Ile Ala Gly Val Val Ser Ala Val Gly Ser Asp Val Thr Lys Trp65 70 75 80Lys Val Gly Asp Arg Val Gly Val Gly Cys Leu Val Asn Ser Cys Gly
85 90 95Glu Cys Glu Gln Cys Val Ala Gly Phe Glu Asn Asn Cys Leu Arg Gly
100 105 110Asn Val Gly Thr Tyr Asn Ser Asn Asp Val Asp Gly Thr Ile Thr Gln
115 120 125Gly Gly Tyr Ala Glu Lys Val Val Val Asn Glu Arg Phe Leu Cys Ser
130 135 140Ile Pro Glu Glu Leu Asn Phe Asp Val Ala Ala Pro Leu Leu Cys Ala145 150 155 160Gly Ile Thr Thr Tyr Ser Pro Ile Ala Arg Trp Asn Val Lys Glu Gly
165 170 175Asp Lys Val Ala Val Met Gly Leu Gly Gly Thr Arg Thr His Gly Cys
180 185 190Pro Asp Arg Cys Ser Gln Gly Cys
195 200<210>213<211>615<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(592)<223>RXA01572<400>213ctgctgtgcg caggcatcac cacctactcc ccaatcgctc gctggaacgt taaagaaggc 60gacaaagtag cagtcatggg cctcggcggg actcggacac atg ggt gtc cag atc 115
Met Gly Val Gln Ile
1 5
gct gca gcc aag ggt gct gag gtt acc gtt ctg tcc cgt tcc ctg cgc 163Ala Ala Ala Lys Gly Ala Glu Val Thr Val Leu Ser Arg Ser Leu Arg
10 15 20aag gca gaa ctt gcc aag gaa ctc ggc gca gct cgc acg ctt gcg act 211Lys Ala Glu Leu Ala Lys Glu Leu Gly Ala Ala Arg Thr Leu Ala Thr
25 30 35tct gat gag gat ttc ttc acc gaa cac gcc ggt gaa ttc gac ttc atc 259Ser Asp Glu Asp Phe Phe Thr Glu His Ala Gly Glu Phe Asp Phe Ile
40 45 50ctc aac acc att agc gca tcc atc cca gtc gac aag tac ctg agc ctt 307Leu Asn Thr Ile Ser Ala Ser Ile Pro Val Asp Lys Tyr Leu Ser Leu
55 60 65ctc aag cca cac ggt gtc atg gct gtt gtc ggt ctg cca cca gag aag 355Leu Lys Pro His Gly Val Met Ala Val Val Gly Leu Pro Pro Glu Lys70 75 80 85cag cca ctg agc ttc ggt gcg ctc atc ggc ggc gga aaa gtc ctc acc 403Gln Pro Leu Ser Phe Gly Ala Leu Ile Gly Gly Gly Lys Val Leu Thr
90 95 100gga tcc aac att ggc ggc atc cct gaa acc cag gaa atg ctc gac ttc 451Gly Ser Asn Ile Gly Gly Ile Pro Glu Thr Gln Glu Met Leu Asp Phe
105 110 115tgt gca aaa cac ggc ctc ggt gcg atg atc gaa act gtc ggc gtc aac 499Cys Ala Lys His Gly Leu Gly Ala Met Ile Glu Thr Val Gly Val Asn
120 125 130gat gtt gat gca gcc tac gac cgt gtt gtt gcc ggc gac gtt cag ttc 547Asp Val Asp Ala Ala Tyr Asp Arg Val Val Ala Gly Asp Val Gln Phe
135 140 145cgc gtt gtc att gat act gct tcg ttt gct gag gtt gag gcg gtt 592Arg Val Val Ile Asp Thr Ala Ser Phe Ala Glu Val Glu Ala Val150 155 160taggtttact gaagttcaga ctt 615<210>214<211>164<212>PRT<213>谷氨酸棒杆菌<400>214Met Gly Val Gln Ile Ala Ala Ala Lys Gly Ala Glu Val Thr Val Leu1 5 10 15Ser Arg Ser Leu Arg Lys Ala Glu Leu Ala Lys Glu Leu Gly Ala Ala
20 25 30Arg Thr Leu Ala Thr Ser Asp Glu Asp Phe Phe Thr Glu His Ala Gly
35 40 45Glu Phe Asp Phe Ile Leu Asn Thr Ile Ser Ala Ser Ile Pro Val Asp
50 55 60Lys Tyr Leu Ser Leu Leu Lys Pro His Gly Val Met Ala Val Val Gly65 70 75 80Leu Pro Pro Glu Lys Gln Pro Leu Ser Phe Gly Ala Leu Ile Gly Gly
85 90 95Gly Lys Val Leu Thr Gly Ser Asn Ile Gly Gly Ile Pro Glu Thr Gln
100 105 110Glu Met Leu Asp Phe cys Ala Lys His Gly Leu Gly Ala Met Ile Glu
115 120 125Thr Val Gly Val Asn Asp Val Asp Ala Ala Tyr Asp Arg Val Val Ala
130 135 140Gly Asp Val Gln Phe Arg Val Val Ile Asp Thr Ala Ser Phe Ala Glu145 150 155 160Val Glu Ala Val<210>215<211>1140<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1117)<223>RXA01758<400>215cccccttatt cagagtgatg gtctaccgga gaagtaccca gaccaatagc atcgaccaac 60gatagcgcgc tcagaagttc tttagtgaaa gcagaaccaa atg ccc aaa tac att 115
Met Pro Lys Tyr Ile
1 5gcc atg cag gta tcc gaa tcc ggt gca ccg tta gcc gcg aat ctc gtg 163Ala Met Gln Val Ser Glu Ser Gly Ala Pro Leu Ala Ala Asn Leu Val
10 15 20caa cct gct ccg ttg aaa tcg agg gaa gtc cgc gtg gaa atc gct gct 211Gln Pro Ala Pro Leu Lys Ser Arg Glu Val Arg Val Glu Ile Ala Ala
25 30 35agt ggt gtg tgc cat gca gat att ggc acg gca gca gca tcg ggg aag 259Ser Gly Val Cys His Ala Asp Ile Gly Thr Ala Ala Ala Ser Gly Lys
40 45 50cac act gtt ttt cct gtt acc cct ggt cat gag att gca gga acc atc 307His Thr Val Phe Pro Val Thr Pro Gly His Glu Ile Ala Gly Thr Ile
55 60 65gcg gaa att ggt gaa aac gta tct cgg tgg acg gtt ggt gat cgc gtt 355Ala Glu Ile Gly Glu Asn Val Ser Arg Trp Thr Val Gly Asp Arg Val70 75 80 85gca atc ggt tgg ttt ggt ggc aat tgc ggt gac tgc gct ttt tgt cgt 403Ala Ile Gly Trp Phe Gly Gly Asn Cys Gly Asp Cys Ala Phe Cys Arg
90 95 100gca ggt gat cct gtg cat tgc aga gag cgg aag att cct ggc gtt tct 451Ala Gly Asp Pro Val His Cys Arg Glu Arg Lys Ile Pro Gly Val Ser
105 110 115tat gcg ggt ggt tgg gca cag aat att gtt gtt cca gcg gag gct ctt 499Tyr Ala Gly Gly Trp Ala Gln Asn Ile Val Val Pro Ala Glu Ala Leu
120 125 130gct gcg att cca gat ggc atg gac ttt tac gag ccc gcc ccg atg ggc 547Ala Ala Ile Pro Asp Gly Met Asp Phe Tyr Glu Pro Ala Pro Met Gly
135 140 145tgc gca ggt gtg aca aca ttc aat gcg ttg cga aac ctg aag ctg gat 595Cys Ala Gly Val Thr Thr Phe Asn Ala Leu Arg Asn Leu Lys Leu Asp150 155 160 165ccc ggt gcg gct gtc gcg gtc ttt gga atc ggc ggt tta gtg cgc cta 643Pro Gly Ala Ala Val Ala Val Phe Gly Ile Gly Gly Leu Val Arg Leu
170 175 180gct att cag ttt gct gcg aaa atg ggt tat cga acc atc acc atc gcc 691Ala Ile Gln Phe Ala Ala Lys Met Gly Tyr Arg Thr Ile Thr Ile Ala
185 190 195cgc ggt tta gag cgt gag gag cta gct agg caa ctt ggc gcc aac cac 739Arg Gly Leu Glu Arg Glu Glu Leu Ala Arg Gln Leu Gly Ala Asn His
200 205 210tac atc gat agc aat gat ctg cac cct ggc cag gcg tta ttt gaa ctt 787Tyr Ile Asp Ser Asn Asp Leu His Pro Gly Gln Ala Leu Phe Glu Leu
215 220 225ggc ggg gct gac ttg atc ttg tct act gcg tcc acc acg gag cct ctt 835Gly Gly Ala Asp Leu Ile Leu Ser Thr Ala Ser Thr Thr Glu Pro Leu230 235 240 245tcg gag ttg tct acc ggt ctt tct att ggc ggg cag cta acc att atc 883Ser Glu Leu Ser Thr Gly Leu Ser Ile Gly Gly Gln Leu Thr Ile Ile
250 255 260gga gtt gat ggg gga gat atc acc gtt tcg gca gcc caa ttg atg atg 931Gly Val Asp Gly Gly Asp Ile Thr Val Ser Ala Ala Gln Leu Met Met
265 270 275aac cgt cag atc atc aca ggt cac ctc act gga agt gcg aat gac acg 979Asn Arg Gln Ile Ile Thr Gly His Leu Thr Gly Ser Ala Asn Asp Thr
280 285 290gaa cag act atg aaa ttt gct cat ctc cat ggc gtg aaa ccg ctt att 1027Glu Gln Thr Met Lys Phe Ala His Leu His Gly Val Lys Pro Leu Ile
295 300 305gaa cgg atg cct ctc gat caa gcc aac gag gct att gca cgt att tca 1075Glu Arg Met Pro Leu Asp Gln Ala Asn Glu Ala Ile Ala Arg Ile Ser310 315 320 325gct ggt aaa cca cgt ttc cgt att gtc ttg gag ccg aat tca 1117Ala Gly Lys Pro Arg Phe Arg Ile Val Leu Glu Pro Asn Ser
330 335taatgccaac agcaagccca att 1140<210>216<211>339<212>PRT<213>谷氨酸棒杆菌<400>216Met Pro Lys Tyr Ile Ala Met Gln Val Ser Glu Ser Gly Ala Pro Leu1 5 10 15Ala Ala Asn Leu Val Gln Pro Ala Pro Leu Lys Ser Arg Glu Val Arg
20 25 30Val Glu Ile Ala Ala Ser Gly Val Cys His Ala Asp Ile Gly Thr Ala
35 40 45Ala Ala Ser Gly Lys His Thr Val Phe Pro Val Thr Pro Gly His Glu
50 55 60Ile Ala Gly Thr Ile Ala Glu Ile Gly Glu Asn Val Ser Arg Trp Thr65 70 75 80Val Gly Asp Arg Val Ala Ile Gly Trp Phe Gly Gly Asn Cys Gly Asp
85 90 95Cys Ala Phe Cys Arg Ala Gly Asp Pro Val His Cys Arg Glu Arg Lys
100 105 110Ile Pro Gly Val Ser Tyr Ala Gly Gly Trp Ala Gln Asn Ile Val Val
115 120 125Pro Ala Glu Ala Leu Ala Ala Ile Pro Asp Gly Met Asp Phe Tyr Glu
130 135 140Pro Ala Pro Met Gly Cys Ala Gly Val Thr Thr Phe Asn Ala Leu Arg145 150 155 160Asn Leu Lys Leu Asp Pro Gly Ala Ala Val Ala Val Phe Gly Ile Gly
165 170 175Gly Leu Val Arg Leu Ala Ile Gln Phe Ala Ala Lys Met Gly Tyr Arg
180 185 190Thr Ile Thr Ile Ala Arg Gly Leu Glu Arg Glu Glu Leu Ala Arg Gln
195 200 205Leu Gly Ala Asn His Tyr Ile Asp Ser Asn Asp Leu His Pro Gly Gln
210 215 220Ala Leu Phe Glu Leu Gly Gly Ala Asp Leu Ile Leu Ser Thr Ala Ser225 230 235 240Thr Thr Glu Pro Leu Ser Glu Leu Ser Thr Gly Leu Ser Ile Gly Gly
245 250 255Gln Leu Thr Ile Ile Gly Val Asp Gly Gly Asp Ile Thr Val Ser Ala
260 265 270Ala Gln Leu Met Met Asn Arg Gln Ile Ile Thr Gly His Leu Thr Gly
275 280 285Ser Ala Asn Asp Thr Glu Gln Thr Met Lys Phe Ala His Leu His Gly
290 295 300Val Lys Pro Leu Ile Glu Arg Met Pro Leu Asp Gln Ala Asn Glu Ala305 310 315 320Ile Ala Arg Ile Ser Ala Gly Lys Pro Arg Phe Arg Ile Val Leu Glu
325 330 335Pro Asn Ser<210>217<211>1641<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1618)<223>RXA02539<400>217ggctgctaag cgtgcgaatg tgcgcgttgt cacaatcgtt gaccaagtgt cacctgacgc 60acaggtagtg ctcaggtgga ggtggcccaa aggagaccca atg act gtc tac gca 115
Met Thr Val Tyr Ala
1 5aat cca gga acc gaa ggc tcg atc gtt aac tat gaa aag cgc tac gag 163Asn Pro Gly Thr Glu Gly Ser Ile Val Asn Tyr Glu Lys Arg Tyr Glu
10 15 20aac tac att ggt ggc aag tgg gtt cca ccg gta gag ggc cag tac ctt 211Asn Tyr Ile Gly Gly Lys Trp Val Pro Pro Val Glu Gly Gln Tyr Leu
25 30 35gag aac att tca cct gtc act ggt gaa gtt ttc tgt gag gtc gca cgt 259Glu Asn Ile Ser Pro Val Thr Gly Glu Val Phe Cys Glu Val Ala Arg
40 45 50ggc acc gca gcg gac gtg gag ctt gca ctg gat gct gca cat gca gcc 307Gly Thr Ala Ala Asp Val Glu Leu Ala Leu Asp Ala Ala His Ala Ala
55 60 65gct gat gcg tgg ggc aag act tct gtc gct gaa cgt gct ctg atc ctg 355Ala Asp Ala Trp Gly Lys Thr Ser Val Ala Glu Arg Ala Leu Ile Leu70 75 80 85cac cgc att gcg gac cgc atg gaa gag cac ctg gaa gaa atc gca gtt 403His Arg Ile Ala Asp Arg Met Glu Glu His Leu Glu Glu Ile Ala Val
90 95 100gca gaa acc tgg gag aac ggc aag gca gtc cgt gag act ctt gct gca 451Ala Glu Thr Trp Glu Asn Gly Lys Ala Val Arg Glu Thr Leu Ala Ala
105 110 115gat atc cca ctg gca atc gac cac ttc cgc tac ttt gct ggc gcg atc 499Asp Ile Pro Leu Ala Ile Asp His Phe Arg Tyr Phe Ala Gly Ala Ile
120 125 130cgt gct cag gaa gat cgt tcc tca cag atc gac cac aac act gtt gct 547Arg Ala Gln Glu Asp Arg Ser Ser Gln Ile Asp His Asn Thr Val Ala
135 140 145tac cac ttc aac gag cca atc ggt gtt gtt ggt cag atc att cct tgg 595Tyr His Phe Asn Glu Pro Ile Gly Val Val Gly Gln Ile Ile Pro Trp150 155 160 165aac ttc cca atc ctc atg gct acc tgg aag ctc gca ccg gca ctt gct 643Asn Phe Pro Ile Leu Met Ala Thr Trp Lys Leu Ala Pro Ala Leu Ala
170 175 180gca ggt aac gcg atc gtc atg aag cca gct gag cag acc cca gca tcc 691Ala Gly Asn Ala Ile Val Met Lys Pro Ala Glu Gln Thr Pro Ala Ser
185 190 195att ttg tat ctg att aac atc atc ggc gat ctc atc cca gag ggc gtc 739Ile Leu Tyr Leu Ile Asn Ile Ile Gly Asp Leu Ile Pro Glu Gly Val
200 205 210ctc aac atc gtc aac gga ctc ggc ggt gaa gca ggc gct gca ctg tcc 787Leu Asn Ile Val Asn Gly Leu Gly Gly Glu Ala Gly Ala Ala Leu Ser
215 220 225ggc tct aat cgg att ggc aag att gct ttc acc ggt tcc acc gag gtc 835Gly Ser Asn Arg Ile Gly Lys Ile Ala Phe Thr Gly Ser Thr Glu Val230 235 240 245ggc aag ctg atc aac cgc gct gca tcc gac aag atc att cct gtc acc 883Gly Lys Leu Ile Asn Arg Ala Ala Ser Asp Lys Ile Ile Pro Val Thr
250 255 260ctg gag ctc ggc ggt aag tcc cca tcc atc ttc ttc tcc gat gtt ctg 931Leu Glu Leu Gly Gly Lys Ser Pro Ser Ile Phe Phe Ser Asp Val Leu
265 270 275tca cag gat gac gcc ttc gca gag aag gca gtt gaa ggc ttc gcg atg 979Ser Gln Asp Asp Ala Phe Ala Glu Lys Ala Val Glu Gly Phe Ala Met
280 285 290ttc gcc ctc aat cag ggt gaa gtt tgt acc tgt cct tcc cgt gca ctt 1027Phe Ala Leu Asn Gln Gly Glu Val Cys Thr Cys Pro Ser Arg Ala Leu
295 300 305gtt cat gag tcc atc gct gat gaa ttc ctc gag ctt ggc gtg aag cga 1075Val His Glu Ser Ile Ala Asp Glu Phe Leu Glu Leu Gly Val Lys Arg310 315 320 325gtt cag aac atc aag ctg ggt aac cca ctt gat act gaa acc atg atg 1123Val Gln Asn Ile Lys Leu Gly Asn Pro Leu Asp Thr Glu Thr Met Met
330 335 340ggt gct cag gcg tcc cag gag cag atg gac aag atc tcc tcc tac ctg 1171Gly Ala Gln Ala Ser Gln Glu Gln Met Asp Lys Ile Ser Ser Tyr Leu
345 350 355aag atc ggc cca gaa gaa ggc gct caa acc ctc act ggt ggc aag gtc 1219Lys Ile Gly Pro Glu Glu Gly Ala Gln Thr Leu Thr Gly Gly Lys Val
360 365 370aac aag gtt gat ggc atg gag aac ggt tac tac att gag cca acc gtt 1267Asn Lys Val Asp Gly Met Glu Asn Gly Tyr Tyr Ile Glu Pro Thr Val
375 380 385ttc cgc ggc acc aac gac atg agg atc ttc cgc gag gaa atc ttc gga 1315Phe Arg Gly Thr Asn Asp Met Arg Ile Phe Arg Glu Glu Ile Phe Gly390 395 400 405cca gtc ctt tct gtt gct acc ttc agc gac ttc gat gag gcc atc cgt 1363Pro Val Leu Ser Val Ala Thr Phe Ser Asp Phe Asp Glu Ala Ile Arg
410 415 420att gca aac gac acc aac tac ggc ctc ggc gct ggt gtc tgg agc cgt 1411Ile Ala Asn Asp Thr Asn Tyr Gly Leu Gly Ala Gly Val Trp Ser Arg
425 430 435gac caa aac acc att tat cgt gca ggt cgc gca atc cag gct ggt cga 1459Asp Gln Asn Thr Ile Tyr Arg Ala Gly Arg Ala Ile Gln Ala Gly Arg
440 445 450gtt tgg gtc aac cag tac cac aac tac cca gcg cac tcc gct ttc ggt 1507Val Trp Val Asn Gln Tyr His Asn Tyr Pro Ala His Ser Ala Phe Gly
455 460 465gga tac aag gag tcc ggc atc ggc cgt gag aac cac ctc atg atg ctg 1555Gly Tyr Lys Glu Ser Gly Ile Gly Arg Glu Asn His Leu Met Met Leu470 475 480 485aac cac tac cag cag acc aag aac ctg ttg gtc tcc tac gat cca aac 1603Asn His Tyr Gln Gln Thr Lys Asn Leu Leu Val Ser Tyr Asp Pro Asn
490 495 500cca acc gga ctg ttc tgatctaagc gttaagtcct aga 1641Pro Thr Gly Leu Phe
505<210>218<211>506<212>PRT<213>谷氨酸棒杆菌<400>218Met Thr Val Tyr Ala Asn Pro Gly Thr Glu Gly Ser Ile Val Asn Tyr1 5 10 15Glu Lys Arg Tyr Glu Asn Tyr Ile Gly Gly Lys Trp Val Pro Pro Val
20 25 30Glu Gly Gln Tyr Leu Glu Asn Ile Ser Pro Val Thr Gly Glu Val Phe
35 40 45Cys Glu Val Ala Arg Gly Thr Ala Ala Asp Val Glu Leu Ala Leu Asp
50 55 60Ala Ala His Ala Ala Ala Asp Ala Trp Gly Lys Thr Ser Val Ala Glu65 70 75 80Arg Ala Leu Ile Leu His Arg Ile Ala Asp Arg Met Glu Glu His Leu
85 90 95Glu Glu Ile Ala Val Ala Glu Thr Trp Glu Asn Gly Lys Ala Val Arg
100 105 110Glu Thr Leu Ala Ala Asp Ile Pro Leu Ala Ile Asp His Phe Arg Tyr
115 120 125Phe Ala Gly Ala Ile Arg Ala Gln Glu Asp Arg Ser Ser Gln Ile Asp
130 135 140His Asn Thr Val Ala Tyr His Phe Asn Glu Pro Ile Gly Val Val Gly145 150 155 160Gln Ile Ile Pro Trp Asn Phe Pro Ile Leu Met Ala Thr Trp Lys Leu
165 170 175Ala Pro Ala Leu Ala Ala Gly Asn Ala Ile Val Met Lys Pro Ala Glu
180 185 190Gln Thr Pro Ala Ser Ile Leu Tyr Leu Ile Asn Ile Ile Gly Asp Leu
195 200 205Ile Pro Glu Gly Val Leu Asn Ile Val Asn Gly Leu Gly Gly Glu Ala
210 215 220Gly Ala Ala Leu Ser Gly Ser Asn Arg Ile Gly Lys Ile Ala Phe Thr225 230 235 240Gly Ser Thr Glu Val Gly Lys Leu Ile Asn Arg Ala Ala Ser Asp Lys
245 250 255Ile Ile Pro Val Thr Leu Glu Leu Gly Gly Lys Ser Pro Ser Ile Phe
260 265 270Phe Ser Asp Val Leu Ser Gln Asp Asp Ala Phe Ala Glu Lys Ala Val
275 280 285Glu Gly Phe Ala Met Phe Ala Leu Asn Gln Gly Glu Val Cys Thr Cys
290 295 300Pro Ser Arg Ala Leu Val His Glu Ser Ile Ala Asp Glu Phe Leu Glu305 310 315 320Leu Gly Val Lys Arg Val Gln Asn Ile Lys Leu Gly Asn Pro Leu Asp
325 330 335Thr Glu Thr Met Met Gly Ala Gln Ala Ser Gln Glu Gln Met Asp Lys
340 345 350Ile Ser Ser Tyr Leu Lys Ile Gly Pro Glu Glu Gly Ala Gln Thr Leu
355 360 365Thr Gly Gly Lys Val Asn Lys Val Asp Gly Met Glu Asn Gly Tyr Tyr
370 375 380Ile Glu Pro Thr Val Phe Arg Gly Thr Asn Asp Met Arg Ile Phe Arg385 390 395 400Glu Glu Ile Phe Gly Pro Val Leu Ser Val Ala Thr Phe Ser Asp Phe
405 410 415Asp Glu Ala Ile Arg Ile Ala Asn Asp Thr Asn Tyr Gly Leu Gly Ala
420 425 430Gly Val Trp Ser Arg Asp Gln Asn Thr Ile Tyr Arg Ala Gly Arg Ala
435 440 445Ile Gln Ala Gly Arg Val Trp Val Asn Gln Tyr His Asn Tyr Pro Ala
450 455 460His Ser Ala Phe Gly Gly Tyr Lys Glu Ser Gly Ile Gly Arg Glu Asn465 470 475 480His Leu Met Met Leu Asn His Tyr Gln Gln Thr Lys Asn Leu Leu Val
485 490 495Ser Tyr Asp Pro Asn Pro Thr Gly Leu Phe
500 505<210>219<211>430<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(430)<223>RXN03061<400>219ctgccaccac tggtcattgc agaggacact ctccgtgatg gtcttcaggt gttagtcgca 60gccctagagc gcgaaaccgc gcaccagaag gtgggctaaa gtg tct ttg acc ttc 115
Val Ser Leu Thr Phe
1 5cca gta atc aac ccc agc gat ggc tcc acc atc acc gag cta gaa aac 163Pro Val Ile Asn Pro Ser Asp Gly Ser Thr Ile Thr Glu Leu Glu Asn
10 15 20cac gat tcc acc cag tgg atg tcc gcg ctc tct gat gca gtt gca gct 211His Asp Ser Thr Gln Trp Met Ser Ala Leu Ser Asp Ala Val Ala Ala
25 30 35ggt cct tca tgg gct gcg aaa act ccc cgc gaa aga tcc gtg gta ctc 259Gly Pro Ser Trp Ala Ala Lys Thr Pro Arg Glu Arg Ser Val Val Leu
40 45 50acc gca atc ttc gaa gca ctg acc gaa cgc gcc caa gaa ctt gca gag 307Thr Ala Ile Phe Glu Ala Leu Thr Glu Arg Ala Gln Glu Leu Ala Glu
55 60 65atc atc cac ctg gaa gct gga aaa tcc gat gca gaa gct ctt ggt gaa 355Ile Ile His Leu Glu Ala Gly Lys Ser Asp Ala Glu Ala Leu Gly Glu70 75 80 85gtc gct tat ggt gca gaa tac ttc cgt tgg ttt gcg gaa gaa gca gtg 403Val Ala Tyr Gly Ala Glu Tyr Phe Arg Trp Phe Ala Glu Glu Ala Val
90 95 100cgc ctg ccc ggc cgc tac gga cag tca 430Arg Leu Pro Gly Arg Tyr Gly Gln Ser
105 110<210>220<211>110<212>PRT<213>谷氨酸棒杆菌<400> 220Val Ser Leu Thr Phe Pro Val Ile Asn Pro Ser Asp Gly Ser Thr Ile1 5 10 15Thr Glu Leu Glu Asn His Asp Ser Thr Gln Trp Met Ser Ala Leu Ser
20 25 30Asp Ala Val Ala Ala Gly Pro Ser Trp Ala Ala Lys Thr Pro Arg Glu
35 40 45Arg Ser Val Val Leu Thr Ala Ile Phe Glu Ala Leu Thr Glu Arg Ala
50 55 60Gln Glu Leu Ala Glu Ile Ile His Leu Glu Ala Gly Lys Ser Asp Ala65 70 75 80Glu Ala Leu Gly Glu Val Ala Tyr Gly Ala Glu Tyr Phe Arg Trp Phe
85 90 95Ala Glu Glu Ala Val Arg Leu Pro Gly Arg Tyr Gly Gln Ser
100 105 110<210>221<211>747<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(724)<223>RXN03150<400>221tttaacagag tgcgtttcaa tgcctgtagt gttccggcaa ttttgaatgt cgttacggtt 60acccaaggct gaattcctga gctcaccttg tacaagatca gtg gaa gcc cag ttc 115
Val Glu Ala Gln Phe
1 5acc tct ccc ctg ctc aac aat ggg caa acc tgt ttc ctt ggt acc cga 163Thr Ser Pro Leu Leu Asn Asn Gly Gln Thr Cys Phe Leu Gly Thr Arg
10 15 20atc ctt gct cca aaa tca cgt tac gcg gaa gta gtc gat gca ttc acc 211Ile Leu Ala Pro Lys Ser Arg Tyr Ala Glu Val Val Asp Ala Phe Thr
25 30 35gct ttc gct ggc agc ctg cag gtt gga gtc acg tcc tcc cct gac act 259Ala Phe Ala Gly Ser Leu Gln Val Gly Val Thr Ser Ser Pro Asp Thr
40 45 50cag atc gga ccg atg gcg act gcc cgg cag cgt gag cgc gtg gaa tcc 307Gln Ile Gly Pro Met Ala Thr Ala Arg Gln Arg Glu Arg Val Glu Ser
55 60 65tac att tcc caa ggc aaa aat gct gga gcc cgc atc act gtc ggt ggc 355Tyr Ile Ser Gln Gly Lys Asn Ala Gly Ala Arg Ile Thr Val Gly Gly70 75 80 85agc cgt cca cga gat ctt gac gcc gga ttc ttc gtt gag cca aca gtg 403Ser Arg Pro Arg Asp Leu Asp Ala Gly Phe Phe Val Glu Pro Thr Val
90 95 100ttc gcc gat gta gac aat cgc gca gcc att gcc caa gat gaa atc ttc 451Phe Ala Asp Val Asp Asn Arg Ala Ala Ile Ala Gln Asp Glu Ile Phe
105 110 115gga ccg gtg ccc tct gtt gtt tcc tac caa gac gat gaa cac gcc atc 499Gly Pro Val Pro Ser Val Val Ser Tyr Gln Asp Asp Glu His Ala Ile
120 125 130caa cta gcc aac gat tcc gaa ttc ggt ctc ggc gga act gtc tgg acg 547Gln Leu Ala Asn Asp Ser Glu Phe Gly Leu Gly Gly Thr Val Trp Thr
135 140 145agc gat ccc gag cgc ggc gct gca ttg gcc cgc cga gtt cac aca gga 595Ser Asp Pro Glu Arg Gly Ala Ala Leu Ala Arg Arg Val His Thr Gly150 155 160 165acc att ggc atc aac cgc tat atc cct gat ccc gcc gca cca ttt gga 643Thr Ile Gly Ile Asn Arg Tyr Ile Pro Asp Pro Ala Ala Pro Phe Gly
170 175 180ggt gtg aaa aac agt ggc ctt ggc aga gaa ctc ggc ccc gaa ggt ctt 691Gly Val Lys Asn Ser Gly Leu Gly Arg Glu Leu Gly Pro Glu Gly Leu
185 190 195gct tcc tac caa gaa acc caa acc att tat ctc taatccaaac tgcacctata 744Ala Ser Tyr Gln Glu Thr Gln Thr Ile Tyr Leu
200 205tat 747<210>222<211>208<212>PRT<213>谷氨酸棒杆菌<400>222Val Glu Ala Gln Phe Thr Ser Pro Leu Leu Asn Asn Gly Gln Thr Cys1 5 10 15Phe Leu Gly Thr Arg Ile Leu Ala Pro Lys Ser Arg Tyr Ala Glu Val
20 25 30Val Asp Ala Phe Thr Ala Phe Ala Gly Ser Leu Gln Val Gly Val Thr
35 40 45Ser Ser Pro Asp Thr Gln Ile Gly Pro Met Ala Thr Ala Arg Gln Arg
50 55 60Glu Arg Val Glu Ser Tyr Ile Ser Gln Gly Lys Asn Ala Gly Ala Arg65 70 75 80Ile Thr Val Gly Gly Ser Arg Pro Arg Asp Leu Asp Ala Gly Phe Phe
85 90 95Val Glu Pro Thr Val Phe Ala Asp Val Asp Asn Arg Ala Ala Ile Ala
100 105 110Gln Asp Glu Ile Phe Gly Pro Val Pro Ser Val Val Ser Tyr Gln Asp
115 120 125Asp Glu His Ala Ile Gln Leu Ala Asn Asp Ser Glu Phe Gly Leu Gly
130 135 140Gly Thr Val Trp Thr Ser Asp Pro Glu Arg Gly Ala Ala Leu Ala Arg145 150 155 160Arg Val His Thr Gly Thr Ile Gly Ile Asn Arg Tyr Ile Pro Asp Pro
165 170 175Ala Ala Pro Phe Gly Gly Val Lys Asn Ser Gly Leu Gly Arg Glu Leu
180 185 190Gly Pro Glu Gly Leu Ala Ser Tyr Gln Glu Thr Gln Thr Ile Tyr Leu
195 200 205<210>223<211>881<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(858)<223>RXN01340<400>223aaa gtg ggg gag atc atc gcc tcc gtc ttt gat acc ttt aat atc ccg 48Lys Val Gly Glu Ile Ile Ala Ser Val Phe Asp Thr Phe Asn Ile Pro1 5 10 15cag ggc ttg gtc tca atc atc acc acc act cga gat gca gag cta tcg 96Gln Gly Leu Val Ser Ile Ile Thr Thr Thr Arg Asp Ala Glu Leu Ser
20 25 30gca gaa ctc atg gct gat cct cgc ttg gct aaa gtc acc ttc act gga 144Ala Glu Leu Met Ala Asp Pro Arg Leu Ala Lys Val Thr Phe Thr Gly
35 40 45tca acc aac gtg gga cgc atc ctg gtc cgc caa tcc gcg gac cga ctg 192Ser Thr Asn Val Gly Arg Ile Leu Val Arg Gln Ser Ala Asp Arg Leu
50 55 60ctg cgc acc tcc atg gaa ctc ggc gga aat gca gct ttt gtt atc gac 240Leu Arg Thr Ser Met Glu Leu Gly Gly Asn Ala Ala Phe Val Ile Asp65 70 75 80gaa gcc gca gac ctc gac gaa gcc gta tcc ggt gcc atc gcc gca aaa 288Glu Ala Ala Asp Leu Asp Glu Ala Val Ser Gly Ala Ile Ala Ala Lys
85 90 95ctc cgc aac gcc ggc caa gta tgc atc gca gct aac cgt ttc ttg gtt 336Leu Arg Asn Ala Gly Gln Val Cys Ile Ala Ala Asn Arg Phe Leu Val
100 105 110cat gaa tcc cgc gct gcc gaa ttc acc tca aag ctg gcg aca gcc atg 384His Glu Ser Arg Ala Ala Glu Phe Thr Ser Lys Leu Ala Thr Ala Met
115 120 125cag aac act ccc att ggg ccg gtg att tct gcc cgc caa cgc gac cgg 432Gln Asn Thr Pro Ile Gly Pro Val Ile Ser Ala Arg Gln Arg Asp Arg
130 135 140atc gca gca cta gtg gat gaa gcc atc acc gac ggc gcc cgc ctc atc 480Ile Ala Ala Leu Val Asp Glu Ala Ile Thr Asp Gly Ala Arg Leu Ile145 150 155 160atc ggt ggg gag gtc ccc gac ggc tcc ggc ttc ttc tat cca gcc acc 528Ile Gly Gly Glu Val Pro Asp Gly Ser Gly Phe Phe Tyr Pro Ala Thr
165 170 175atc ttg gcc gat gtc cct gca cag tca cgg att gtg cat gag gaa atc 576Ile Leu Ala Asp Val Pro Ala Gln Ser Arg Ile Val His Glu Glu Ile
180 185 190ttc gga cct gtg gcc acc att gcc act ttc acc gac ttg gcc gaa ggc 624Phe Gly Pro Val Ala Thr Ile Ala Thr Phe Thr Asp Leu Ala Glu Gly
195 200 205gtt gca caa gca aat tcc acc gaa ttc ggc ctc gca gcc tac gga ttc 672Val Ala Gln Ala Asn Ser Thr Glu Phe Gly Leu Ala Ala Tyr Gly Phe
210 215 220agc aac aat gtg aaa gca aca cag tac atg gcg gaa cac ttg gaa gcc 720Ser Asn Asn Val Lys Ala Thr Gln Tyr Met Ala Glu His Leu Glu Ala225 230 235 240gga atg gtc gga atc aac aga ggc gcc atc tct gac cca gca gca cct 768Gly Met Val Gly Ile Asn Arg Gly Ala Ile Ser Asp Pro Ala Ala Pro
245 250 255ttt ggc ggc atc gga caa tcc ggc ttc ggc aga gaa ggc gga acc gaa 816Phe Gly Gly Ile Gly Gln Ser Gly Phe Gly Arg Glu Gly Gly Thr Glu
260 265 270gga atc gaa gaa tat ctc tcc gtg cgt tac ctc gct ttg ccg 858Gly Ile Glu Glu Tyr Leu Ser Val Arg Tyr Leu Ala Leu Pro
275 280 285tgacacatga gctgtccggt gaa 881<210>224<211>286<212>PRT<213>谷氨酸棒杆菌<400>224Lys Val Gly Glu Ile Ile Ala Ser Val Phe Asp Thr Phe Asn Ile Pro1 5 10 15Gln Gly Leu Val Ser Ile Ile Thr Thr Thr Arg Asp Ala Glu Leu Ser
20 25 30Ala Glu Leu Met Ala Asp Pro Arg Leu Ala Lys Val Thr Phe Thr Gly
35 40 45Ser Thr Asn Val Gly Arg Ile Leu Val Arg Gln Ser Ala Asp Arg Leu
50 55 60Leu Arg Thr Ser Met Glu Leu Gly Gly Asn Ala Ala Phe Val Ile Asp65 70 75 80Glu Ala Ala Asp Leu Asp Glu Ala Val Ser Gly Ala Ile Ala Ala Lys
85 90 95Leu Arg Asn Ala Gly Gln Val Cys Ile Ala Ala Asa Arg Phe Leu Val
100 105 110His Glu Ser Arg Ala Ala Glu Phe Thr Ser Lys Leu Ala Thr Ala Met
115 120 125Gln Asn Thr Pro Ile Gly Pro Val Ile Ser Ala Arg Gln Arg Asp Arg
130 135 140Ile Ala Ala Leu Val Asp Glu Ala Ile Thr Asp Gly Ala Arg Leu Ile145 150 155 160Ile Gly Gly Glu Val Pro Asp Gly Ser Gly Phe Phe Tyr Pro Ala Thr
165 170 175Ile Leu Ala Asp Val Pro Ala Gln Ser Arg Ile Val His Glu Glu Ile
180 185 190Phe Gly Pro Val Ala Thr Ile Ala Thr Phe Thr Asp Leu Ala Glu Gly
195 200 205Val Ala Gln Ala Asn Ser Thr Glu Phe Gly Leu Ala Ala Tyr Gly Phe
210 215 220Ser Asn Asn Val Lys Ala Thr Gln Tyr Met Ala Glu His Leu Glu Ala225 230 235 240Gly Met Val Gly Ile Asn Arg Gly Ala Ile Ser Asp Pro Ala Ala Pro
245 250 255Phe Gly Gly Ile Gly Gln Ser Gly Phe Gly Arg Glu Gly Gly Thr Glu
260 265 270Gly Ile Glu Glu Tyr Leu Ser Val Arg Tyr Leu Ala Leu Pro
275 280 285<210>225<211>1686<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1663)<223>RXN01498<400>225cagtggacaa ctacttggcg ggtcttaaat cagctgtgaa ggattctgca taagctgggc 60accacacgag catcagaacg cgaaacgaag gtaaaagccc atg atc aaa cgt ctt 115
Met Ile Lys Arg Leu
1 5cct tta ggt ccg ctg cct aaa gaa ctt cat cag act ctg ctt gat ctg 163Pro Leu Gly Pro Leu Pro Lys Glu Leu His Gln Thr Leu Leu Asp Leu
10 15 20acc gca aat gcc caa gat gcg gcg aaa gtg gag gtt ata gcg cca ttt 211Thr Ala Asn Ala Gln Asp Ala Ala Lys Val Glu Val Ile Ala Pro Phe
25 30 35act ggc gag acc ctc gga ttt ggt ttt gat ggt gat gag caa gac gtc 259Thr Gly Glu Thr Leu Gly Phe Gly Phe Asp Gly Asp Glu Gln Asp Val
40 45 50gag cat gct ttt gca ctt tca agg gca gcc cag aaa aag tgg gtg cac 307Glu His Ala Phe Ala Leu Ser Arg Ala Ala Gln Lys Lys Trp Val His
55 60 65acc acg gca gtg gaa cgg aag aag atc ttc ctg aag gtt cat gat ctg 355Thr Thr Ala Val Glu Arg Lys Lys Ile Phe Leu Lys Val His Asp Leu70 75 80 85gta ttg aaa aac cgt gag ctg ctc atg gac atc gtg cag ttg gaa aca 403Val Leu Lys Asn Arg Glu Leu Leu Met Asp Ile Val Gln Leu Glu Thr
90 95 100ggc aaa aat cga gca tcg gct gcc gat gag gtg ttg gac gtt gcg atc 451Gly Lys Asn Arg Ala Ser Ala Ala Asp Glu Val Leu Asp Val Ala Ile
105 110 115acc acc cgc ttc tac gca aac aat gca gga aag ttt tta aat gac aag 499Thr Thr Arg Phe Tyr Ala Asn Asn Ala Gly Lys Phe Leu Asn Asp Lys
120 125 130aaa cgc ccc ggc gcg ctt ccg atc atc acg aaa aac aca caa cag tat 547Lys Arg Pro Gly Ala Leu Pro Ile Ile Thr Lys Asn Thr Gln Gln Tyr
135 140 145gtg ccc aag gga gtg gtc ggg cag atc acg ccg tgg aat tac cct tta 595Val Pro Lys Gly Val Val Gly Gln Ile Thr Pro Trp Asn Tyr Pro Leu150 155 160 165act ttg gga gta tct gat gct gtt ccg gcg ctg ctg gca gga aac gca 643Thr Leu Gly Val Ser Asp Ala Val Pro Ala Leu Leu Ala Gly Asn Ala
170 175 180gtg gtg gct aaa cct gac ctc gcg aca cct ttc tcc tgc ttg atc atg 691Val Val Ala Lys Pro Asp Leu Ala Thr Pro Phe Ser Cys Leu Ile Met
185 190 195gtg cac ctg ctc att gaa gcc ggt ctg ccg cgt gat ttg atg cag gtt 739Val His Leu Leu Ile Glu Ala Gly Leu Pro Arg Asp Leu Met Gln Val
200 205 210gtc acc ggc cct ggc gat att gtt ggc ggt gcg att gca gct cag tgt 787Val Thr Gly Pro Gly Asp Ile Val Gly Gly Ala Ile Ala Ala Gln Cys
215 220 225gat ttc ctc atg ttc act gga tcc acg gcc acg ggc cgg atc ttg ggt 835Asp Phe Leu Met Phe Thr Gly Ser Thr Ala Thr Gly Arg Ile Leu Gly230 235 240 245cgg aca atg ggt gag cgt ttg gtg ggt ttc tct gcg gaa tta ggc gga 883Arg Thr Met Gly Glu Arg Leu Val Gly Phe Ser Ala Glu Leu Gly Gly
250 255 260aag aac cct ctt att gtg gcc aag gat gca gat ctg gac aag gtg gaa 931Lys Asn Pro Leu Ile Val Ala Lys Asp Ala Asp Leu Asp Lys Val Glu
265 270 275gct gag ctt ccg cag gcg tgt ttt tcc aac tcg ggg caa ttg tgt gtc 979Ala Glu Leu Pro Gln Ala Cys Phe Ser Asn Ser Gly Gln Leu Cys Val
280 285 290tcc act gaa cgt att tat gtc gag gaa gac gtg tac gag gag gtg att 1027Ser Thr Glu Arg Ile Tyr Val Glu Glu Asp Val Tyr Glu Glu Val Ile
295 300 305gca cgg ttt agc aag gcg gcg aaa gcc atg tcc att ggt gcc gga ttt 1075Ala Arg Phe Ser Lys Ala Ala Lys Ala Met Ser Ile Gly Ala Gly Phe310 315 320 325gag tgg aaa tat gag atg ggt tcg ttg atc aat cac gcg cag ctg gat 1123Glu Trp Lys Tyr Glu Met Gly Ser Leu Ile Asn His Ala Gln Leu Asp
330 335 340cgg gtg agc acc ttt gtt gat cag gct aaa gct gcg ggc gcc acg gtg 1171Arg Val Ser Thr Phe Val Asp Gln Ala Lys Ala Ala Gly Ala Thr Val
345 350 355ctg tgc ggt ggc aag tca cgc cct gat att ggt ccc ttc ttc tat gag 1219Leu Cys Gly Gly Lys Ser Arg Pro Asp Ile Gly Pro Phe Phe Tyr Glu
360 365 370ccc acg gta ttg gcg gat gtc cca gag ggc acc cca ctg ctc acg gag 1267Pro Thr Val Leu Ala Asp Val Pro Glu Gly Thr Pro Leu Leu Thr Glu
375 380 385gaa gtc ttc ggg ccg gtg gtg ttc atc gaa aag gta gcc aca ctg gaa 1315Glu Val Phe Gly Pro Val Val Phe Ile Glu Lys Val Ala Thr Leu Glu390 395 400 405gaa gcc gtc gat aag gca aat ggc acg ccc tac ggc ctg aat gcg tcc 1363Glu Ala Val Asp Lys Ala Asn Gly Thr Pro Tyr Gly Leu Asn Ala Ser
410 415 420gtc ttt ggg tcg tcg gaa acc ggc aat ctt gtt gca ggc cag ctg gaa 1411Val Phe Gly Ser Ser Glu Thr Gly Asn Leu Val Ala Gly Gln Leu Glu
425 430 435gct ggc ggt atc ggt att aat gat ggc tac gcc gcg acg tgg gcg agc 1459Ala Gly Gly Ile Gly Ile Asn Asp Gly Tyr Ala Ala Thr Trp Ala Ser
440 445 450gtg tcc acg cct ctg ggt ggc atg aag cag tcg ggg ctg ggg cac cgc 1507Val Ser Thr Pro Leu Gly Gly Met Lys Gln Ser Gly Leu Gly His Arg
455 460 465cat ggt gcg gag gga att aca aaa tat gcg gag atc cga aac atc gcg 1555His Gly Ala Glu Gly Ile Thr Lys Tyr Ala Glu Ile Arg Asn Ile Ala470 475 480 485gag cag cgc tgg atg tct atg cgt ggg ccg gcc aaa atg ccg cga aag 1603Glu Gln Arg Trp Met Ser Met Arg Gly Pro Ala Lys Met Pro Arg Lys
490 495 500gtg tac tca gac acc gtg gcc aca gcg cta aag ctg ggc aaa atc ttt 1651Val Tyr Ser Asp Thr Val Ala Thr Ala Leu Lys Leu Gly Lys Ile Phe
505 510 515aaa gtt ttg ccg tagcaaaaag ccggaccctt gct 1686Lys Val Leu Pro
520<210>226<211>521<212>PRT<213>谷氨酸棒杆菌<400>226Met Ile Lys Arg Leu Pro Leu Gly Pro Leu Pro Lys Glu Leu His Gln1 5 10 15Thr Leu Leu Asp Leu Thr Ala Asn Ala Gln Asp Ala Ala Lys Val Glu
20 25 30Val Ile Ala Pro Phe Thr Gly Glu Thr Leu Gly Phe Gly Phe Asp Gly
35 40 45Asp Glu Gln Asp Val Glu His Ala Phe Ala Leu Ser Arg Ala Ala Gln
50 55 60Lys Lys Trp Val His Thr Thr Ala Val Glu Arg Lys Lys Ile Phe Leu65 70 75 80Lys Val His Asp Leu Val Leu Lys Asn Arg Glu Leu Leu Met Asp Ile
85 90 95Val Gln Leu Glu Thr Gly Lys Asn Arg Ala Ser Ala Ala Asp Glu Val
100 105 110Leu Asp Val Ala Ile Thr Thr Arg Phe Tyr Ala Asn Asn Ala Gly Lys
115 120 125Phe Leu Asn Asp Lys Lys Arg Pro Gly Ala Leu Pro Ile Ile Thr Lys
130 135 140Asn Thr Gln Gln Tyr Val Pro Lys Gly Val Val Gly Gln Ile Thr Pro145 150 155 160Trp Asn Tyr Pro Leu Thr Leu Gly Val Ser Asp Ala Val Pro Ala Leu
165 170 175Leu Ala Gly Asn Ala Val Val Ala Lys Pro Asp Leu Ala Thr Pro Phe
180 185 190Ser Cys Leu Ile Met Val His Leu Leu Ile Glu Ala Gly Leu Pro Arg
195 200 205Asp Leu Met Gln Val Val Thr Gly Pro Gly Asp Ile Val Gly Gly Ala
210 215 220Ile Ala Ala Gln Cys Asp Phe Leu Met Phe Thr Gly Ser Thr Ala Thr225 230 235 240Gly Arg Ile Leu Gly Arg Thr Met Gly Glu Arg Leu Val Gly Phe Ser
245 250 255Ala Glu Leu Gly Gly Lys Asn Pro Leu Ile Val Ala Lys Asp Ala Asp
260 265 270Leu Asp Lys Val Glu Ala Glu Leu Pro Gln Ala Cys Phe Ser Asn Ser
275 280 285Gly Gln Leu Cys Val Ser Thr Glu Arg Ile Tyr Val Glu Glu Asp Val
290 295 300Tyr Glu Glu Val Ile Ala Arg Phe Ser Lys Ala Ala Lys Ala Met Ser305 310 315 320Ile Gly Ala Gly Phe Glu Trp Lys Tyr Glu Met Gly Ser Leu Ile Asn
325 330 335His Ala Gln Leu Asp Arg Val Ser Thr Phe Val Asp Gln Ala Lys Ala
340 345 350Ala Gly Ala Thr Val Leu Cys Gly Gly Lys Ser Arg Pro Asp Ile Gly
355 360 365Pro Phe Phe Tyr Glu Pro Thr Val Leu Ala Asp Val Pro Glu Gly Thr
370 375 380Pro Leu Leu Thr Glu Glu Val Phe Gly Pro Val Val Phe Ile Glu Lys385 390 395 400Val Ala Thr Leu Glu Glu Ala Val Asp Lys Ala Asn Gly Thr Pro Tyr
405 410 415Gly Leu Asn Ala Ser Val Phe Gly Ser Ser Glu Thr Gly Asn Leu Val
420 425 430Ala Gly Gln Leu Glu Ala Gly Gly Ile Gly Ile Asn Asp Gly Tyr Ala
435 440 445Ala Thr Trp Ala Ser Val Ser Thr Pro Leu Gly Gly Met Lys Gln Ser
450 455 460Gly Leu Gly His Arg His Gly Ala Glu Gly Ile Thr Lys Tyr Ala Glu465 470 475 480Ile Arg Asn Ile Ala Glu Gln Arg Trp Met Ser Met Arg Gly Pro Ala
485 490 495Lys Met Pro Arg Lys Val Tyr Ser Asp Thr Val Ala Thr Ala Leu Lys
500 505 510Leu Gly Lys Ile Phe Lys Val Leu Pro
515 520<210>227<211>1575<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1552)<223>RXN02674<400>227atcgacctct agggcagcag tgattgattt cataaaaatc acaagtttgt aactaaaggt 60acagttggtg aagtatccac aatcaacttt aggagacctt gtg act gca aca ttt 115
Val Thr Ala Thr Phe
1 5gct gga atc gac gcc acc aaa cac ctc atc gga ggt cag tgg gtg gag 163Ala Gly Ile Asp Ala Thr Lys His Leu Ile Gly Gly Gln Trp Val Glu
10 15 20gga aac tcg gat cga att tcc acc aat atc aat cct tac gac gat tcc 211Gly Asn Ser Asp Arg Ile Ser Thr Asn Ile Asn Pro Tyr Asp Asp Ser
25 30 35gta atc gcc gaa agc aaa caa gct tcc att gct gat gtt gat gcc gcg 259Val Ile Ala Glu Ser Lys Gln Ala Ser Ile Ala Asp Val Asp Ala Ala
40 45 50tat gaa gcc gcg aag aag gcc cag gct gag tgg gca gct acg ccc gct 307Tyr Glu Ala Ala Lys Lys Ala Gln Ala Glu Trp Ala Ala Thr Pro Ala
55 60 65gcg gaa cga tct gcc atc atc tac cgt gcg gct gaa ctt ctt gaa gag 355Ala Glu Arg Ser Ala Ile Ile Tyr Arg Ala Ala Glu Leu Leu Glu Glu70 75 80 85cac cgg gag gaa atc gtg gaa tgg ctg atc aag gaa tcc ggc tcg acg 403His Arg Glu Glu Ile Val Glu Trp Leu Ile Lys Glu Ser Gly Ser Thr
90 95 100cgt tcc aag gct aat ttg gaa atc act ttg gca gga aac atc act aaa 451Arg Ser Lys Ala Asn Leu Glu Ile Thr Leu Ala Gly Asn Ile Thr Lys
105 110 115gaa tcg gct tca ttc cct ggt cgt gtg cat ggt cga att tct cct tcg 499Glu Ser Ala Ser Phe Pro Gly Arg Val His Gly Arg Ile Ser Pro Ser
120 125 130aat act ccg ggc aaa gaa aac cgt gtg tac cgc gta gcc aag ggc gtt 547Asn Thr Pro Gly Lys Glu Asn Arg Val Tyr Arg Val Ala Lys Gly Val
135 140 145gtc gga gtg att agt cca tgg aat ttc cca ctg aac ctc tcg atc cgc 595Val Gly Val Ile Ser Pro Trp Asn Phe Pro Leu Asn Leu Ser Ile Arg150 155 160 165tcg gtt gct ccg gca cta gcc gtg ggc aac gcc gta gtg att aag cct 643Ser Val Ala Pro Ala Leu Ala Val Gly Asn Ala Val Val Ile Lys Pro
170 175 180gcg agt gat acc cca gtt act ggt ggt gta att cct gca cga atc ttt 691Ala Ser Asp Thr Pro Val Thr Gly Gly Val Ile Pro Ala Arg Ile Phe
185 190 195gag gag gcc gga gtt cct gca ggc gtg atc agc acg gtt gcg ggc gca 739Glu Glu Ala Gly Val Pro Ala Gly Val Ile Ser Thr Val Ala Gly Ala
200 205 210gga tct gaa atc ggt gat cac ttt gtc acc cac gcc gtg cca aag ctg 787Gly Ser Glu Ile Gly Asp His Phe Val Thr His Ala Val Pro Lys Leu
215 220 225att tct ttc acc ggt tca acc cca gtc ggt cgt cgt gtc ggt gag ctg 835Ile Ser Phe Thr Gly Ser Thr Pro Val Gly Arg Arg Val Gly Glu Leu230 235 240 245gca att aat ggt gga cca atg aaa act gtt gca cta gag ctc ggt ggc 883Ala Ile Asn Gly Gly Pro Met Lys Thr Val Ala Leu Glu Leu Gly Gly
250 255 260aac gcg ccg ttc gtt gtg ctt gcc gac gcc gac atc gac gcc gct gcc 931Asn Ala Pro Phe Val Val Leu Ala Asp Ala Asp Ile Asp Ala Ala Ala
265 270 275cag gct gcc gca gtt ggc gct ttc cta cac cag gga cag att tgt atg 979Gln Ala Ala Ala Val Gly Ala Phe Leu His Gln Gly Gln Ile Cys Met
280 285 290tca atc aac cga gtc att gtt gat gct gca gtt cat gat gaa ttc cta 1027Ser Ile Asn Arg Val Ile Val Asp Ala Ala Val His Asp Glu Phe Leu
295 300 305gag aag ttc gtt gaa gca gtg aag aac att cca acc ggc gat cca agc 1075Glu Lys Phe Val Glu Ala Val Lys Asn Ile Pro Thr Gly Asp Pro Ser310 315 320 325gca gaa gga acc ctt gtt gga cct gtc att aat gac agt cag ctc agt 1123Ala Glu Gly Thr Leu Val Gly Pro Val Ile Asn Asp Ser Gln Leu Ser
330 335 340ggt ttg aag gaa aag atc gag ttg gcc aaa aag gaa ggc gca acc gtc 1171Gly Leu Lys Glu Lys Ile Glu Leu Ala Lys Lys Glu Gly Ala Thr Val
345 350 355cag gtt gaa ggg cca att gaa ggc cga ctg gtt cat ccg cat gtg ttc 1219Gln Val Glu Gly Pro Ile Glu Gly Arg Leu Val His Pro His Val Phe
360 365 370tct gat gtc acc tct gac atg gaa atc gct cgt gag gaa atc ttc gga 1267Ser Asp Val Thr Ser Asp Met Glu Ile Ala Arg Glu Glu Ile Phe Gly
375 380 385cct ctc atc agc gtg ctg aag gcc gat gat gag gca cac gca gca gag 1315Pro Leu Ile Ser Val Leu Lys Ala Asp Asp Glu Ala His Ala Ala Glu390 395 400 405ctg gcc aat gct tcc gac ttt ggt ttg agc gcg gca gtg tgg tcg aag 1363Leu Ala Asn Ala Ser Asp Phe Gly Leu Ser Ala Ala Val Trp Ser Lys
410 415 420gat att gat cgt gca gcc cag ttt gct ctg cag att gat tcc ggc atg 1411Asp Ile Asp Arg Ala Ala Gln Phe Ala Leu Gln Ile Asp Ser Gly Met
425 430 435gtt cac atc aat gac ctc acc gtc aac gat gaa cca cac gtg atg ttc 1459Val His Ile Asn Asp Leu Thr Val Asn Asp Glu Pro His Val Met Phe
440 445 450ggt ggt tca aag aac tct ggc ctc ggc cgc ttc aac ggc gat tgg gcg 1507Gly Gly Ser Lys Asn Ser Gly Leu Gly Arg Phe Asn Gly Asp Trp Ala
455 460 465atc gag gag ttc acc aca gat cga tgg atc ggc atc aag cgc agc 1552Ile Glu Glu Phe Thr Thr Asp Arg Trp Ile Gly Ile Lys Arg Ser470 475 480taattgtttt tcgacgtaac ccc 1575<210>228<211>484<212>PRT<213>谷氨酸棒杆菌<400>228Val Thr Ala Thr Phe Ala Gly Ile Asp Ala Thr Lys His Leu Ile Gly1 5 10 15Gly Gln Trp Val Glu Gly Asn Ser Asp Arg Ile Ser Thr Asn Ile Asn
20 25 30Pro Tyr Asp Asp Ser Val Ile Ala Glu Ser Lys Gln Ala Ser Ile Ala
35 40 45Asp Val Asp Ala Ala Tyr Glu Ala Ala Lys Lys Ala Gln Ala Glu Trp
50 55 60Ala Ala Thr Pro Ala Ala Glu Arg Ser Ala Ile Ile Tyr Arg Ala Ala65 70 75 80Glu Leu Leu Glu Glu His Arg Glu Glu Ile Val Glu Trp Leu Ile Lys
85 90 95Glu Ser Gly Ser Thr Arg Ser Lys Ala Asn Leu Glu Ile Thr Leu Ala
100 105 110Gly Asn Ile Thr Lys Glu Ser Ala Ser Phe Pro Gly Arg Val His Gly
115 120 125Arg Ile Ser Pro Ser Asn Thr Pro Gly Lys Glu Asn Arg Val Tyr Arg
130 135 140Val Ala Lys Gly Val Val Gly Val Ile Ser Pro Trp Asn Phe Pro Leu145 150 155 160Asn Leu Ser Ile Arg Ser Val Ala Pro Ala Leu Ala Val Gly Asn Ala
165 170 175Val Val Ile Lys Pro Ala Ser Asp Thr Pro Val Thr Gly Gly Val Ile
180 185 190Pro Ala Arg Ile Phe Glu Glu Ala Gly Val Pro Ala Gly Val Ile Ser
195 200 205Thr Val Ala Gly Ala Gly Ser Glu Ile Gly Asp His Phe Val Thr His
210 215 220Ala Val Pro Lys Leu Ile Ser Phe Thr Gly Ser Thr Pro Val Gly Arg225 230 235 240Arg Val Gly Glu Leu Ala Ile Asn Gly Gly Pro Met Lys Thr Val Ala
245 250 255Leu Glu Leu Gly Gly Asn Ala Pro Phe Val Val Leu Ala Asp Ala Asp
260 265 270Ile Asp Ala Ala Ala Gln Ala Ala Ala Val Gly Ala Phe Leu His Gln
275 280 285Gly Gln Ile Cys Met Ser Ile Asn Arg Val Ile Val Asp Ala Ala Val
290 295 300His Asp Glu Phe Leu Glu Lys Phe Val Glu Ala Val Lys Asn Ile Pro305 310 315 320Thr Gly Asp Pro Ser Ala Glu Gly Thr Leu Val Gly Pro Val Ile Asn
325 330 335Asp Ser Gln Leu Ser Gly Leu Lys Glu Lys Ile Glu Leu Ala Lys Lys
340 345 350Glu Gly Ala Thr Val Gln Val Glu Gly Pro Ile Glu Gly Arg Leu Val
355 360 365His Pro His Val Phe Ser Asp Val Thr Ser Asp Met Glu Ile Ala Arg
370 375 380Glu Glu Ile Phe Gly Pro Leu Ile Ser Val Leu Lys Ala Asp Asp Glu385 390 395 400Ala His Ala Ala Glu Leu Ala Asn Ala Ser Asp Phe Gly Leu Ser Ala
405 410 415Ala Val Trp Ser Lys Asp Ile Asp Arg Ala Ala Gln Phe Ala Leu Gln
420 425 430Ile Asp Ser Gly Met Val His Ile Asn Asp Leu Thr Val Asn Asp Glu
435 440 445Pro His Val Met Phe Gly Gly Ser Lys Asn Ser Gly Leu Gly Arg Phe
450 455 460Asn Gly Asp Trp Ala Ile Glu Glu Phe Thr Thr Asp Arg Trp Ile Gly465 470 475 480Ile Lys Arg Ser<210>229<211>2034<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2011)<223>RXN00868<400>229cgatgaccca gcgcacgcct gggttcgaga tacatggacc gggcaagcat ttgatgatcg 60cttgccatat gagaacgcaa acaaggaggg ataaaatttc atg gct gaa acg aag 115
Met Ala Glu Thr Lys
1 5aga atg aca gtt agc cag gca ctg gtt gaa ttc ctt ggt cac cag tgg 163Arg Met Thr Val Ser Gln Ala Leu Val Glu Phe Leu Gly His Gln Trp
10 15 20act gtc gac ggc gat atc cgc gag cgc acc att cca ggc atg ttc gga 211Thr Val Asp Gly Asp Ile Arg Glu Arg Thr Ile Pro Gly Met Phe Gly
25 30 35att ttc gga cac gga aac gtt gct ggc att ggc cag gca ctc aag cag 259Ile Phe Gly His Gly Asn Val Ala Gly Ile Gly Gln Ala Leu Lys Gln
40 45 50tac aac gtt gaa caa cct gag ctc atg ccg tac tac cag gct cgt aat 307Tyr Asn Val Glu Gln Pro Glu Leu Met Pro Tyr Tyr Gln Ala Arg Asn
55 60 65gag cag gcg atg gtg cac cag tct gtt gga tat gca cgc atg cac cgc 355Glu Gln Ala Met Val His Gln Ser Val Gly Tyr Ala Arg Met His Arg70 75 80 85cgt cgt ggc aca tac gca tct gcc gca tct gtt gga ccc ggc gcg acc 403Arg Arg Gly Thr Tyr Ala Ser Ala Ala Ser Val Gly Pro Gly Ala Thr
90 95 100aac ctg tta acc ggt gcg gct ctt gct acc acc aac cgt ttg cca gcg 451Asn Leu Leu Thr Gly Ala Ala Leu Ala Thr Thr Asn Arg Leu Pro Ala
105 110 115ttg ctg ctg cct agt gat act ttt gcc acc cgc gtg gcg gat cca gtg 499Leu Leu Leu Pro Ser Asp Thr Phe Ala Thr Arg Val Ala Asp Pro Val
120 125 130ttg cag cag ttg gag cag cca tgg gat atc ggg ctg acg gtt aat gat 547Leu Gln Gln Leu Glu Gln Pro Trp Asp Ile Gly Leu Thr Val Asn Asp
135 140 145gct ttc cgc cct gtg tct aag ttc ttt gat cgg gtg cag cgc ccg gag 595Ala Phe Arg Pro Val Ser Lys Phe Phe Asp Arg Val Gln Arg Pro Glu150 155 160 165cag ttg ttc tct att gcg ttg gct gcg atg cgt gtg ttg act gat ccc 643Gln Leu Phe Ser Ile Ala Leu Ala Ala Met Arg Val Leu Thr Asp Pro
170 175 180gca gaa acc ggt gcg gtc acc att gcg ctt cca gaa gat gtg cag gct 691Ala Glu Thr Gly Ala Val Thr Ile Ala Leu Pro Glu Asp Val Gln Ala
185 190 195gaa atg ctc gat gtg ccg gtg gag ttc ttg cag gat cgt gag tgg cac 739Glu Met Leu Asp Val Pro Val Glu Phe Leu Gln Asp Arg Glu Trp His
200 205 210att agg cgc cca cgt cca gag cgt gct gcg ttg gct cgt gcg att gaa 787Ile Arg Arg Pro Arg Pro Glu Arg Ala Ala Leu Ala Arg Ala Ile Glu
215 220 225gtc atc aaa aac gct aag aat ccg atg atc att gct ggt ggc gga gtg 835Val Ile Lys Asn Ala Lys Asn Pro Met Ile Ile Ala Gly Gly Gly Val230 235 240 245ttg tac tcc gat gcg gaa acg cag ctg cag gca ctt gtg gag cag act 883Leu Tyr Ser Asp Ala Glu Thr Gln Leu Gln Ala Leu Val Glu Gln Thr
250 255 260ggc att cca gtg ggt acc tcc caa gct ggt ggt ggc gtg ttg gcg tgg 931Gly Ile Pro Val Gly Thr Ser Gln Ala Gly Gly Gly Val Leu Ala Trp
265 270 275gat cat gca caa aac tta ggt ggt gtg ggt gcc acc gga acg ttg gct 979Asp His Ala Gln Asn Leu Gly Gly Val Gly Ala Thr Gly Thr Leu Ala
280 285 290gcc aac cgc att gcg ggt gat gct gat gtg atc atc ggt atc ggt act 1027Ala Asn Arg Ile Ala Gly Asp Ala Asp Val Ile Ile Gly Ile Gly Thr
295 300 305cgt tac agc gat ttc acc acc gca tct cgc act gcg ttc caa aac cct 1075Arg Tyr Ser Asp Phe Thr Thr Ala Ser Arg Thr Ala Phe Gln Asn Pro310 315 320 325gat gtc acc ttc atc aac atc aat gtt gct tcc ttc gat gct tac aag 1123Asp Val Thr Phe Ile Asn Ile Asn Val Ala Ser Phe Asp Ala Tyr Lys
330 335 340cat ggc act cag ttg cct gtg att gca gat gca cgc gag gca att gtg 1171His Gly Thr Gln Leu Pro Val Ile Ala Asp Ala Arg Glu Ala Ile Val
345 350 355gag ctt gct gaa gcc ctg cag gga ttc acc gtg gca gag gat tac gcg 1219Glu Leu Ala Glu Ala Leu Gln Gly Phe Thr Val Ala Glu Asp Tyr Ala
360 365 370cag cgc atc gcg aag gaa aag gct gcg tgg gac gca gaa gta gat aag 1267Gln Arg Ile Ala Lys Glu Lys Ala Ala Trp Asp Ala Glu Val Asp Lys
375 380 385tct ttt gcc ccc tcc ggt ctt gcg ctg cct gga cag ccg gag atc atc 1315Ser Phe Ala Pro Ser Gly Leu Ala Leu Pro Gly Gln Pro Glu Ile Ile390 395 400 405ggc gcg gtg cag gcg tcg aca agc gaa aaa gac gtc att gtg cag gcc 1363Gly Ala Val Gln Ala Ser Thr Ser Glu Lys Asp Val Ile Val Gln Ala
410 415 420gct gga tcc ttg cct ggt gac ctg cac aag ctg tgg cgt gtg cgc gat 1411Ala Gly Ser Leu Pro Gly Asp Leu His Lys Leu Trp Arg Val Arg Asp
425 430 435gcg ctg ggc tac cac gtg gaa tat gcg ttc tcg tgc atg ggc tat gaa 1459Ala Leu Gly Tyr His Val Glu Tyr Ala Phe Ser Cys Met Gly Tyr Glu
440 445 450atc gcg ggc ggt atc ggc gcg aag cgt gga ctt gat gcc gca ggc gat 1507Ile Ala Gly Gly Ile Gly Ala Lys Arg Gly Leu Asp Ala Ala Gly Asp
455 460 465gac cgc gac gtg gtg atc atg gtt ggt gat ggg tcc tac ctc atg ctc 1555Asp Arg Asp Val Val Ile Met Val Gly Asp Gly Ser Tyr Leu Met Leu470 475 480 485aac act gag ctg gtc acg gcc gtg gca gaa ggt atc aag gtg att gtg 1603Asn Thr Glu Leu Val Thr Ala Val Ala Glu Gly Ile Lys Val Ile Val
490 495 500gtg ctc atc caa aac cac ggt tat gcc tcc atc ggc cac ctg tct gaa 1651Val Leu Ile Gln Asn His Gly Tyr Ala Ser Ile Gly His Leu Ser Glu
505 510 515act gtc ggt tcg cag cgt ttt ggt act tgg tac cgc gaa tat gac gct 1699Thr Val Gly Ser Gln Arg Phe Gly Thr Trp Tyr Arg Glu Tyr Asp Ala
520 525 530gag gcg aaa aac ttc cag ggc gag cag att ctg cct gtt gac ctg gcg 1747Glu Ala Lys Asn Phe Gln Gly Glu Gln Ile Leu Pro Val Asp Leu Ala
535 540 545atg aat gca cgc agc tac ggc atg gat gtc att gaa gtg gaa cca agc 1795Met Asn Ala Arg Ser Tyr Gly Met Asp Val Ile Glu Val Glu Pro Ser550 555 560 565gcg aat gcg atc gag gat ctc aaa gca gcg atg gca acc gcg aag gct 1843Ala Asn Ala Ile Glu Asp Leu Lys Ala Ala Met Ala Thr Ala Lys Ala
570 575 580tcg gag aaa tcc acc ttc atc cac atc aac agc gat ccg ttg atc tac 1891Ser Glu Lys Ser Thr Phe Ile His Ile Asn Ser Asp Pro Leu Ile Tyr
585 590 595gca cca gac ggt gct ggt tgg tgg gac gtg ccg gtg tcg gag acg tcc 1939Ala Pro Asp Gly Ala Gly Trp Trp Asp Val Pro Val Ser Glu Thr Ser
600 605 610act ctg gat agc acc aac gcg gct cgt gaa gat tac ctg aaa aac caa 1987Thr Leu Asp Ser Thr Asn Ala Ala Arg Glu Asp Tyr Leu Lys Asn Gln
615 620 625gcc ctc cag cgt ccg ctg ctc ggc taaaccagtt ggctaaacca aaa 2034Ala Leu Gln Arg Pro Leu Leu Gly630 635<210>230<211>637<212>PRT<213>谷氨酸棒杆菌<400>230Met Ala Glu Thr Lys Arg Met Thr Val Ser Gln Ala Leu Val Glu Phe1 5 10 15Leu Gly His Gln Trp Thr Val Asp Gly Asp Ile Arg Glu Arg Thr Ile
20 25 30Pro Gly Met Phe Gly Ile Phe Gly His Gly Asn Val Ala Gly Ile Gly
35 40 45Gln Ala Leu Lys Gln Tyr Asn Val Glu Gln Pro Glu Leu Met Pro Tyr
50 55 60Tyr Gln Ala Arg Asn Glu Gln Ala Met Val His Gln Ser Val Gly Tyr65 70 75 80Ala Arg Met His Arg Arg Arg Gly Thr Tyr Ala Ser Ala Ala Ser Val
85 90 95Gly Pro Gly Ala Thr Asn Leu Leu Thr Gly Ala Ala Leu Ala Thr Thr
100 105 110Asn Arg Leu Pro Ala Leu Leu Leu Pro Ser Asp Thr Phe Ala Thr Arg
115 120 125Val Ala Asp Pro Val Leu Gln Gln Leu Glu Gln Pro Trp Asp Ile Gly
130 135 140Leu Thr Val Asn Asp Ala Phe Arg Pro Val Ser Lys Phe Phe Asp Arg145 150 155 160Val Gln Arg Pro Glu Gln Leu Phe Ser Ile Ala Leu Ala Ala Met Arg
165 170 175Val Leu Thr Asp Pro Ala Glu Thr Gly Ala Val Thr Ile Ala Leu Pro
180 185 190Glu Asp Val Gln Ala Glu Met Leu Asp Val Pro Val Glu Phe Leu Gln
195 200 205Asp Arg Glu Trp His Ile Arg Arg Pro Arg Pro Glu Arg Ala Ala Leu
210 215 220Ala Arg Ala Ile Glu Val Ile Lys Asn Ala Lys Asn Pro Met Ile Ile225 230 235 240Ala Gly Gly Gly Val Leu Tyr Ser Asp Ala Glu Thr Gln Leu Gln Ala
245 250 255Leu Val Glu Gln Thr Gly Ile Pro Val Gly Thr Ser Gln Ala Gly Gly
260 265 270Gly Val Leu Ala Trp Asp His Ala Gln Asn Leu Gly Gly Val Gly Ala
275 280 285Thr Gly Thr Leu Ala Ala Asn Arg Ile Ala Gly Asp Ala Asp Val Ile
290 295 300Ile Gly Ile Gly Thr Arg Tyr Ser Asp Phe Thr Thr Ala Ser Arg Thr305 310 315 320Ala Phe Gln Asn Pro Asp Val Thr Phe Ile Asn Ile Asn Val Ala Ser
325 330 335Phe Asp Ala Tyr Lys His Gly Thr Gln Leu Pro Val Ile Ala Asp Ala
340 345 350Arg Glu Ala Ile Val Glu Leu Ala Glu Ala Leu Gln Gly Phe Thr Val
355 360 365Ala Glu Asp Tyr Ala Gln Arg Ile Ala Lys Glu Lys Ala Ala Trp Asp
370 375 380Ala Glu Val Asp Lys Ser Phe Ala Pro Ser Gly Leu Ala Leu Pro Gly385 390 395 400Gln Pro Glu Ile Ile Gly Ala Val Gln Ala Ser Thr Ser Glu Lys Asp
405 410 415Val Ile Val Gln Ala Ala Gly Ser Leu Pro Gly Asp Leu His Lys Leu
420 425 430Trp Arg Val Arg Asp Ala Leu Gly Tyr His Val Glu Tyr Ala Phe Ser
435 440 445Cys Met Gly Tyr Glu Ile Ala Gly Gly Ile Gly Ala Lys Arg Gly Leu
450 455 460Asp Ala Ala Gly Asp Asp Arg Asp Val Val Ile Met Val Gly Asp Gly465 470 475 480Ser Tyr Leu Met Leu Asn Thr Glu Leu Val Thr Ala Val Ala Glu Gly
485 490 495Ile Lys Val Ile Val Val Leu Ile Gln Asn His Gly Tyr Ala Ser Ile
500 505 510Gly His Leu Ser Glu Thr Val Gly Ser Gln Arg Phe Gly Thr Trp Tyr
515 520 525Arg Glu Tyr Asp Ala Glu Ala Lys Asn Phe Gln Gly Glu Gln Ile Leu
530 535 540Pro Val AsP Leu Ala Met Asn Ala Arg Ser Tyr Gly Met AsP Val Ile545 550 555 560Glu Val Glu Pro Ser Ala Asn Ala Ile Glu Asp Leu Lys Ala Ala Met
565 570 575Ala Thr Ala Lys Ala Ser Glu Lys Ser Thr Phe Ile His Ile Asn Ser
580 585 590Asp Pro Leu Ile Tyr Ala Pro Asp Gly Ala Gly Trp Trp Asp Val Pro
595 600 605Val Ser Glu Thr Ser Thr Leu Asp Ser Thr Asn Ala Ala Arg Glu Asp
610 615 620Tyr Leu Lys Asn Gln Ala Leu Gln Arg Pro Leu Leu Gly625 630 635<210>231<211>1142<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1119)<223>RXN01143<400>231atc cca gtt gtc acc acc ttg atg gct ttg ggt act ttc cca gag tct 48Ile Pro Val Val Thr Thr Leu Met Ala Leu Gly Thr Phe Pro Glu Ser1 5 10 15cac gag ctg cac atg ggt atg cca ggc atg cat ggc act gtg tcc gct 96His Glu Leu His Met Gly Met Pro Gly Met His Gly Thr Val Ser Ala
20 25 30gtt ggt gca ctg cag cgc agc gac ctg ctg att gct atc ggc tcc cgc 144Val Gly Ala Leu Gln Arg Ser Asp Leu Leu Ile Ala Ile Gly Ser Arg
35 40 45ttt gat gac cgc gtc acc ggt gac gtt gac acc ttc gcg cct gac gcc 192Phe Asp Asp Arg Val Thr Gly Asp Val Asp Thr Phe Ala Pro Asp Ala
50 55 60aag atc att cac gcc gac att gat cct gcc gaa atc ggc aag atc aag 240Lys Ile Ile His Ala Asp Ile Asp Pro Ala Glu Ile Gly Lys Ile Lys65 70 75 80cag gtt gag gtt cca atc gtg ggc gat gcc cgc gaa gtt ctt gct cgt 288Gln Val Glu Val Pro Ile Val Gly Asp Ala Arg Glu Val Leu Ala Arg
85 90 95ctg ctg gaa acc acc aag gca agc aag gca gag acc gag gac atc tcc 336Leu Leu Glu Thr Thr Lys Ala Ser Lys Ala Glu Thr Glu Asp Ile Ser
100 105 110gag tgg gtt gac tac ctc aag ggc ctc aag gca cgt ttc ccg cgt ggc 384Glu Trp Val Asp Tyr Leu Lys Gly Leu Lys Ala Arg Phe Pro Arg Gly
115 120 125tac gac gag cag cca ggc gat ctg ctg gca cca cag ttt gtc att gaa 432Tyr Asp Glu Gln Pro Gly Asp Leu Leu Ala Pro Gln Phe Val Ile Glu
130 135 140acc ctg tcc aag gaa gtt ggc ccc gac gca att tac tgc gcc ggc gtt 480Thr Leu Ser Lys Glu Val Gly Pro Asp Ala Ile Tyr Cys Ala Gly Val145 150 155 160ggc cag cac caa atg tgg gca gct cag ttc gtt gac ttt gaa aag cca 528Gly Gln His Gln Met Trp Ala Ala Gln Phe Val Asp Phe Glu Lys Pro
165 170 175cgc acc tgg ctc aac tcc ggt gga ctg ggc acc atg ggc tac gca gtt 576Arg Thr Trp Leu Asn Ser Gly Gly Leu Gly Thr Met Gly Tyr Ala Val
180 185 190cct gcg gcc ctt gga gca aag gct ggc gca cct gac aag gaa gtc tgg 624Pro Ala Ala Leu Gly Ala Lys Ala Gly Ala Pro Asp Lys Glu Val Trp
195 200 205gct atc gac ggc gac ggc tgt ttc cag atg acc aac cag gaa ctc acc 672Ala Ile Asp Gly Asp Gly Cys Phe Gln Met Thr Asn Gln Glu Leu Thr
210 215 220acc gcc gca gtt gaa ggt ttc ccc att aag atc gca cta atc aac aac 720Thr Ala Ala Val Glu Gly Phe Pro Ile Lys Ile Ala Leu Ile Asn Asn225 230 235 240gga aaa cct ggg gca tgg gtt cgc caa tgg cag acc cta ttc tat gaa 768Gly Lys Pro Gly Ala Trp Val Arg Gln Trp Gln Thr Leu Phe Tyr Glu
245 250 255gga cgg tac tca aat act aaa ctt cgt aac cag ggc gag tac atg ccc 816Gly Arg Tyr Ser Asn Thr Lys Leu Arg Asn Gln Gly Glu Tyr Met Pro
260 265 270gac ttt gtt acc ctt tct gag gga ctt ggc tgt gtt gcc atc cgc gtc 864Asp Phe Val Thr Leu Ser Glu Gly Leu Gly Cys Val Ala Ile Arg Val
275 280 285acc aaa gcg gag gaa gta ctg cca gcc atc caa aag gct cga gag atc 912Thr Lys Ala Glu Glu Val Leu Pro Ala Ile Gln Lys Ala Arg Glu Ile
290 295 300aac gac cgc cca gta gtc atc gac ttc atc gtc ggt gaa gac gca cag 960Asn Asp Arg Pro Val Val Ile Asp Phe Ile Val Gly Glu Asp Ala Gln305 310 315 320gta tgg cca atg gtg tct gct gga tca tcc aac tcc gat atc cag tac 1008Val Trp Pro Met Val Ser Ala Gly Ser Ser Asn Ser Asp Ile Gln Tyr
325 330 335gca ctc gga ttg cgc cca ttc ttt gat ggt gat gaa tct gca gca gaa 1056Ala Leu Gly Leu Arg Pro Phe Phe Asp Gly Asp Glu Ser Ala Ala Glu
340 345 350gat cct gcc gac att cac gaa gcc gtc agc gac att gat gcc gcc gtt 1104Asp Pro Ala Asp Ile His Glu Ala Val Ser Asp Ile Asp Ala Ala Val
355 360 365gaa tcg acc gag gca taaggagaga cccaagatgg cta 1142Glu Ser Thr Glu Ala
370<210>232<211>373<212>PRT<213>谷氨酸棒杆菌<400>232Ile Pro Val Val Thr Thr Leu Met Ala Leu Gly Thr Phe Pro Glu Ser1 5 10 15His Glu Leu His Met Gly Met Pro Gly Met His Gly Thr Val Ser Ala
20 25 30Val Gly Ala Leu Gln Arg Ser Asp Leu Leu Ile Ala Ile Gly Ser Arg
35 40 45Phe Asp Asp Arg Val Thr Gly Asp Val Asp Thr Phe Ala Pro Asp Ala
50 55 60Lys Ile Ile His Ala Asp Ile Asp Pro Ala Glu Ile Gly Lys Ile Lys65 70 75 80Gln Val Glu Val Pro Ile Val Gly Asp Ala Arg Glu Val Leu Ala Arg
85 90 95Leu Leu Glu Thr Thr Lys Ala Ser Lys Ala Glu Thr Glu Asp Ile Ser
100 105 110Glu Trp Val Asp Tyr Leu Lys Gly Leu Lys Ala Arg Phe Pro Arg Gly
115 120 125Tyr Asp Glu Gln Pro Gly Asp Leu Leu Ala Pro Gln Phe Val Ile Glu
130 135 140Thr Leu Ser Lys Glu Val Gly Pro Asp Ala Ile Tyr Cys Ala Gly Val145 150 155 160Gly Gln His Gln Met Trp Ala Ala Gln Phe Val Asp Phe Glu Lys Pro
165 170 175Arg Thr Trp Leu Asn Ser Gly Gly Leu Gly Thr Met Gly Tyr Ala Val
180 185 190Pro Ala Ala Leu Gly Ala Lys Ala Gly Ala Pro Asp Lys Glu Val Trp
195 200 205Ala Ile Asp Gly Asp Gly Cys Phe Gln Met Thr Asn Gln Glu Leu Thr
210 215 220Thr Ala Ala Val Glu Gly Phe Pro Ile Lys Ile Ala Leu Ile Asn Asn225 230 235 240Gly Lys Pro Gly Ala Trp Val Arg Gln Trp Gln Thr Leu Phe Tyr Glu
245 250 255Gly Arg Tyr Ser Asn Thr Lys Leu Arg Asn Gln Gly Glu Tyr Met Pro
260 265 270Asp Phe Val Thr Leu Ser Glu Gly Leu Gly Cys Val Ala Ile Arg Val
275 280 285Thr Lys Ala Glu Glu Val Leu Pro Ala Ile Gln Lys Ala Arg Glu Ile
290 295 300Asn Asp Arg Pro Val Val Ile Asp Phe Ile Val Gly Glu Asp Ala Gln305 310 315 320Val Trp Pro Met Val Ser Ala Gly Ser Ser Asn Ser Asp Ile Gln Tyr
325 330 335Ala Leu Gly Leu Arg Pro Phe Phe Asp Gly Asp Glu Ser Ala Ala Glu
340 345 350Asp Pro Ala Asp Ile His Glu Ala Val Ser Asp Ile Asp Ala Ala Val
355 360 365Glu Ser Thr Glu Ala
370<210>233<211>793<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(793)<223>RXN01146<400>233tatttagtaa aggagccaga aagtcgtgaa tgtggcagct tctcaacagc ccactcccgc 60cacggttgca agccgtggtc gatccgccgc ccctgagcgg atg aca ggt gca aag 115
Met Thr Gly Ala Lys
1 5gca att gtt cga tcg ctc gag gag ctt aac gcc gac atc gtg ttc ggt 163Ala Ile Val Arg Ser Leu Glu Glu Leu Asn Ala Asp Ile Val Phe Gly
10 15 20att cct ggt ggt gcg gtg cta ccg gtg tat gac ccg ctc tat tcc tcc 211Ile Pro Gly Gly Ala Val Leu Pro Val Tyr Asp Pro Leu Tyr Ser Ser
25 30 35aca aag gtg cgc cac gtc ttg gtg cgc cac gag cag ggc gca ggc cac 259Thr Lys Val Arg His Val Leu Val Arg His Glu Gln Gly Ala Gly His
40 45 50gca gca acc ggc tac gcg cag gtt act gga cgc gtt ggc gtc tgc att 307Ala Ala Thr Gly Tyr Ala Gln Val Thr Gly Arg Val Gly Val Cys Ile
55 60 65gca acc tct ggc cca gga gca acc aac ttg gtt acc cca atc gct gat 355Ala Thr Ser Gly Pro Gly Ala Thr Asn Leu Val Thr Pro Ile Ala Asp70 75 80 85gca aac ttg gac tcc gtt ccc atg gtt gcc atc acc ggc cag gtc gga 403Ala Asn Leu Asp Ser Val Pro Met Val Ala Ile Thr Gly Gln Val Gly
90 95 100agt ggc ctg ctg ggt acc gac gct ttc cag gaa gcc gat atc cgc ggc 451Ser Gly Leu Leu Gly Thr Asp Ala Phe Gln Glu Ala Asp Ile Arg Gly
105 110 115atc acc atg cca gtg acc aag cac aac ttc atg gtc acc aac cct aac 499Ile Thr Met Pro Val Thr Lys His Asn Phe Met Val Thr Asn Pro Asn
120 125 130gac att cca cag gca ttg gct gag gca ttc cac ctc gcg att act ggt 547Asp Ile Pro Gln Ala Leu Ala Glu Ala Phe His Leu Ala Ile Thr Gly
135 140 145cgc cct ggc cct gtt ctg gtg gat att cct aag gat gtc cag aac gct 595Arg Pro Gly Pro Val Leu Val Asp Ile Pro Lys Asp Val Gln Asn Ala150 155 160 165gaa ttg gat ttc gtc tgg cca cca aag atc gac ctg cca ggc tac cgc 643Glu Leu Asp Phe Val Trp Pro Pro Lys Ile Asp Leu Pro Gly Tyr Arg
170 175 180cca gtt tca aca cca cat gct cgc cag atc gag cag gca gtc aag ctg 691Pro Val Ser Thr Pro His Ala Arg Gln Ile Glu Gln Ala Val Lys Leu
185 190 195atc ggt gag gcc aag aag ccc gtc ctt tac gtt ggt ggt ggc gta atc 739Ile Gly Glu Ala Lys Lys Pro Val Leu Tyr Val Gly Gly Gly Val Ile
200 205 210aag gct gac gca cac gaa gag ctt cgt gcg ttc gct gag tac acc ggc 787Lys Ala Asp Ala His Glu Glu Leu Arg Ala Phe Ala Glu Tyr Thr Gly
215 220 225atc cca 793Ile Pro230<210>234<211>231<212>PRT<213>谷氨酸棒杆菌<400>234Met Thr Gly Ala Lys Ala Ile Val Arg Ser Leu Glu Glu Leu Asn Ala1 5 10 15Asp Ile Val Phe Gly Ile Pro Gly Gly Ala Val Leu Pro Val Tyr Asp
20 25 30Pro Leu Tyr Ser Ser Thr Lys Val Arg His Val Leu Val Arg His Glu
35 40 45Gln Gly Ala Gly His Ala Ala Thr Gly Tyr Ala Gln Val Thr Gly Arg
50 55 60Val Gly Val Cys Ile Ala Thr Ser Gly Pro Gly Ala Thr Asn Leu Val65 70 75 80Thr Pro Ile Ala Asp Ala Asn Leu Asp Ser Val Pro Met Val Ala Ile
85 90 95Thr Gly Gln Val Gly Ser Gly Leu Leu Gly Thr Asp Ala Phe Gln Glu
100 105 110Ala Asp Ile Arg Gly Ile Thr Met Pro Val Thr Lys His Asn Phe Met
115 120 125Val Thr Asn Pro Asn Asp Ile Pro Gln Ala Leu Ala Glu Ala Phe His
130 135 140Leu Ala Ile Thr Gly Arg Pro Gly Pro Val Leu Val Asp Ile Pro Lys145 150 155 160Asp Val Gln Asn Ala Glu Leu Asp Phe Val Trp Pro Pro Lys Ile Asp
165 170 175Leu Pro Gly Tyr Arg Pro Val Ser Thr Pro His Ala Arg Gln Ile Glu
180 185 190Gln Ala Val Lys Leu Ile Gly Glu Ala Lys Lys Pro Val Leu Tyr Val
195 200 205Gly Gly Gly Val Ile Lys Ala Asp Ala His Glu Glu Leu Arg Ala Phe
210 215 220Ala Glu Tyr Thr Gly Ile Pro225 230<210>235<211>639<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(616)<223>RXN01144<400>235ggtgatgaat ctgcagcaga agatcctgcc gacattcacg aagccgtcag cgacattgat 60gccgccgttg aatcgaccga ggcataagga gagacccaag atg gct aat tct gac 115
Met Ala Asn Ser Asp
1 5gtc acc cgc cac atc ctg tcc gta ctc gtt cag gac gta gac gga atc 163Val Thr Arg His Ile Leu Ser Val Leu Val Gln Asp Val Asp Gly Ile
10 15 20att tcc cgc gta tca ggt atg ttc acc cga cgc gca ttc aac ctc gtg 211Ile Ser Arg Val Ser Gly Met Phe Thr Arg Arg Ala Phe Asn Leu Val
25 30 35ttc ctc gtg tct gca aag acc gaa aca cac ggc atc aac cgc atc acg 259Phe Leu Val Ser Ala Lys Thr Glu Thr His Gly Ile Asn Arg Ile Thr
40 45 50gtt gtt gtc gac gcc gac gag ctc aac att gag cag atc acc aag cag 307Val Val Val Asp Ala Asp Glu Leu Asn Ile Glu Gln Ile Thr Lys Gln
55 60 65ctc aac aag ctg atc ccc gtg ctc aaa gtc gtg cga ctt gat gaa gag 355Leu Asn Lys Leu Ile Pro Val Leu Lys Val Val Arg Leu Asp Glu Glu70 75 80 85acc act atc gcc cgc gca atc atg ctg gtt aag gtc tct gcg gac agc 403Thr Thr Ile Ala Arg Ala Ile Met Leu Val Lys Val Ser Ala Asp Ser
90 95 100acc aac cgt ccg cag atc gtc gac gcc gcg aac atc ttc cgc gcc cga 451Thr Asn Arg Pro Gln Ile Val Asp Ala Ala Asn Ile Phe Arg Ala Arg
105 110 115gtc gtc gac gtg gct cca gac tct gtg gtt att gaa tcc aca ggc acc 499Val Val Asp Val Ala Pro Asp Ser Val Val Ile Glu Ser Thr Gly Thr
120 125 130cca ggc aag ctc cgc gca ctg ctt gac gtg atg gaa cca ttc gga atc 547Pro Gly Lys Leu Arg Ala Leu Leu Asp Val Met Glu Pro Phe Gly Ile
135 140 145cgc gaa ctg atc caa tcc gga cag att gca ctc aac cgc ggt ccg aag 595Arg Glu Leu Ile Gln Ser Gly Gln Ile Ala Leu Asn Arg Gly Pro Lys150 155 160 165acc atg gct ccg gcc aag atc taaacagcaa ttaatctgat tgc 639Thr Met Ala Pro Ala Lys Ile
170<210>236<211>172<212>PRT<213>谷氨酸棒杆菌<400>236Met Ala Asn Ser Asp Val Thr Arg His Ile Leu Ser Val Leu Val Gln1 5 10 15Asp Val Asp Gly Ile Ile Ser Arg Val Ser Gly Met Phe Thr Arg Arg
20 25 30Ala Phe Asn Leu Val Phe Leu Val Ser Ala Lys Thr Glu Thr His Gly
35 40 45Ile Asn Arg Ile Thr Val Val Val Asp Ala Asp Glu Leu Asn Ile Glu
50 55 60Gln Ile Thr Lys Gln Leu Asn Lys Leu Ile Pro Val Leu Lys Val Val65 70 75 80Arg Leu Asp Glu Glu Thr Thr Ile Ala Arg Ala Ile Met Leu Val Lys
85 90 95Val Ser Ala Asp Ser Thr Asn Arg Pro Gln Ile Val Asp Ala Ala Asn
100 105 110Ile Phe Arg Ala Arg Val Val Asp Val Ala Pro Asp Ser Val Val Ile
115 120 125Glu Ser Thr Gly Thr Pro Gly Lys Leu Arg Ala Leu Leu Asp Val Met
130 135 140Glu Pro Phe Gly Ile Arg Glu Leu Ile Gln Ser Gly Gln Ile Ala Leu145 150 155 160Asn Arg Gly Pro Lys Thr Met Ala Pro Ala Lys Ile
165 170<210>237<211>897<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(874)<223>RXA02474<400>237tgctggtcta ttgtggcgac cgagggcctt tgaaggttcg acaaactgta taaggccttg 60aatcttgaga atttattttg aggaagcaag aggaagtgtc atg agc aaa gtt gca 115
Met Ser Lys Val Ala
1 5atg gtt acc ggt ggt gca caa ggc atc ggt cgt gga att tca gag aag 163Met Val Thr Gly Gly Ala Gln Gly Ile Gly Arg Gly Ile Ser Glu Lys
10 15 20ctg gca gca gat ggt ttc gat att gcc gta gcc gac ctg cca caa cag 211Leu Ala Ala Asp Gly Phe Asp Ile Ala Val Ala Asp Leu Pro Gln Gln
25 30 35gaa gaa caa gct gca gag acc atc aag ttg att gaa gct gca ggt caa 259Glu Glu Gln Ala Ala Glu Thr Ile Lys Leu Ile Glu Ala Ala Gly Gln
40 45 50aag gct gta ttc gtt gga tta gat gtc acc gat aag gct aat ttc gac 307Lys Ala Val Phe Val Gly Leu Asp Val Thr Asp Lys Ala Asn Phe Asp
55 60 65agt gca att gat gag gca gca gag aaa ctt ggc ggc ttc gat gtg cta 355Ser Ala Ile Asp Glu Ala Ala Glu Lys Leu Gly Gly Phe Asp Val Leu70 75 80 85gta aac aac gcc ggc atc gca caa att aag cca ctt ctg gaa gtc acc 403Val Asn Asn Ala Gly Ile Ala Gln Ile Lys Pro Leu Leu Glu Val Thr
90 95 100gaa gaa gac cta aag cag atc tac tcc gtg aac gtt ttt agc gta ttt 451Glu Glu Asp Leu Lys Gln Ile Tyr Ser Val Asn Val Phe Ser Val Phe
105 110 115ttt ggt att caa gca gca tcc cga aag ttc gat gag ctt ggc gta aaa 499Phe Gly Ile Gln Ala Ala Ser Arg Lys Phe Asp Glu Leu Gly Val Lys
120 125 130ggc aag atc atc aac gct gca tca atc gct gct atc caa ggt ttc cca 547Gly Lys Ile Ile Asn Ala Ala Ser Ile Ala Ala Ile Gln Gly Phe Pro
135 140 145atc ttg agc gcc tac tcc acc acc aaa ttc gcg gtt cgt ggc ctc acc 595Ile Leu Ser Ala Tyr Ser Thr Thr Lys Phe Ala Val Arg Gly Leu Thr150 155 160 165cag gct gct gcg caa gaa ctc gca ccc aag ggt cac acc gtg aat gcc 643Gln Ala Ala Ala Gln Glu Leu Ala Pro Lys Gly His Thr Val Asn Ala
170 175 180tac gca cct ggc atc gtg ggc acc gga atg tgg gag caa atc gat gcc 691Tyr Ala Pro Gly Ile Val Gly Thr Gly Met Trp Glu Gln Ile Asp Ala
185 190 195gag ctt tcc aag atc aac ggc aag cca atc ggt gag aac ttc aag gag 739Glu Leu Ser Lys Ile Asn Gly Lys Pro Ile Gly Glu Asn Phe Lys Glu
200 205 210tac tcc tcc tca atc gca ttg ggc cga cca tca gta cct gag gat gta 787Tyr Ser Ser Ser Ile Ala Leu Gly Arg Pro Ser Val Pro Glu Asp Val
215 220 225gcc ggt ctg gtt tcg ttc ctg gct tct gaa aac tcc aac tac atc acc 835Ala Gly Leu Val Ser Phe Leu Ala Ser Glu Asn Ser Asn Tyr Ile Thr230 235 240 245gga cag gtc atg ctt gtc gac ggc ggc atg ctc tac aac taggggttgc 884Gly Gln Val Met Leu Val Asp Gly Gly Met Leu Tyr Asn
250 255tttcccgcac tca 897<210>238<211>258<212>PRT<213>谷氨酸棒杆菌<400>238Met Ser Lys Val Ala Met Val Thr Gly Gly Ala Gln Gly Ile Gly Arg1 5 10 15Gly Ile Ser Glu Lys Leu Ala Ala Asp Gly Phe Asp Ile Ala Val Ala
20 25 30Asp Leu Pro Gln Gln Glu Glu Gln Ala Ala Glu Thr Ile Lys Leu Ile
35 40 45Glu Ala Ala Gly Gln Lys Ala Val Phe Val Gly Leu Asp Val Thr Asp
50 55 60Lys Ala Asn Phe Asp Ser Ala Ile Asp Glu Ala Ala Glu Lys Leu Gly65 70 75 80Gly Phe Asp Val Leu Val Asn Asn Ala Gly Ile Ala Gln Ile Lys Pro
85 90 95Leu Leu Glu Val Thr Glu Glu Asp Leu Lys Gln Ile Tyr Ser Val Asn
100 105 110Val Phe Ser Val Phe Phe Gly Ile Gln Ala Ala Ser Arg Lys Phe Asp
115 120 125Glu Leu Gly Val Lys Gly Lys Ile Ile Asn Ala Ala Ser Ile Ala Ala
130 135 140Ile Gln Gly Phe Pro Ile Leu Ser Ala Tyr Ser Thr Thr Lys Phe Ala145 150 155 160Val Arg Gly Leu Thr Gln Ala Ala Ala Gln Glu Leu Ala Pro Lys Gly
165 170 175His Thr Val Asn Ala Tyr Ala Pro Gly Ile Val Gly Thr Gly Met Trp
180 185 190Glu Gln Ile Asp Ala Glu Leu Ser Lys Ile Asn Gly Lys Pro Ile Gly
195 200 205Glu Asn Phe Lys Glu Tyr Ser Ser Ser Ile Ala Leu Gly Arg Pro Ser
210 215 220Val Pro Glu Asp Val Ala Gly Leu Val Ser Phe Leu Ala Ser Glu Asn225 230 235 240Ser Asn Tyr Ile Thr Gly Gln Val Met Leu Val Asp Gly Gly Met Leu
245 250 255Tyr Asn<210>239<211>876<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(853)<223>RXA02453<400>239aaccaacaaa ggtcatctca accggcttaa gaaaattctg ccagctttct gctgattgaa 60tcgtgccagc tcagggcata tctcacctaa agtaaacacc atg aaa tca atc ttc 115
Met Lys Ser Ile Phe
1 5att tcc ggt gcg gcg aac gga att ggc aaa gct gtg gcg ttg aaa ttt 163Ile Ser Gly Ala Ala Asn Gly Ile Gly Lys Ala Val Ala Leu Lys Phe
10 15 20ctt cac gaa ggt tgg ctc gtt gga gcc tac gac ctc gcg gaa atc acc 211Leu His Glu Gly Trp Leu Val Gly Ala Tyr Asp Leu Ala Glu Ile Thr
25 30 35tac tca cac ccc aat ctt cgc tgg ggc tac ctc aat gtt cga cag tcc 259Tyr Ser His Pro Asn Leu Arg Trp Gly Tyr Leu Asn Val Arg Gln Ser
40 45 50gag tcg tgg gac aaa gcc cta gaa gac ttt gcg acg cac acc gga ggc 307Glu Ser Trp Asp Lys Ala Leu Glu Asp Phe Ala Thr His Thr Gly Gly
55 60 65acc atc gat gtg gtg gac aat aat gcc ggc gta att att gag gga ccg 355Thr Ile Asp Val Val Asp Asn Asn Ala Gly Val Ile Ile Glu Gly Pro70 75 80 85ctg cag gac gca gag gag ggg agc gtc gac aag ctt ctt gca atc aac 403Leu Gln Asp Ala Glu Glu Gly Ser Val Asp Lys Leu Leu Ala Ile Asn
90 95 100gtc aat ggc gtg act ctt ggt gcc cgc gcc gct cat cct tat ttg gcg 451Val Asn Gly Val Thr Leu Gly Ala Arg Ala Ala His Pro Tyr Leu Ala
105 110 115cgc acg ccg ggc gcc cag ttg tta aac atg tcc tcg gcg tcg gcg gtg 499Arg Thr Pro Gly Ala Gln Leu Leu Asn Met Ser Ser Ala Ser Ala Val
120 125 130tac ggg cag ccc cag atc gcg gtg tat tcg gct tcg aag ttt tac gtc 547Tyr Gly Gln Pro Gln Ile Ala Val Tyr Ser Ala Ser Lys Phe Tyr Val
135 140 145gca ggt ctt act gag gcg ctg aat ttg gag tgg cgg aaa gac gat att 595Ala Gly Leu Thr Glu Ala Leu Asn Leu Glu Trp Arg Lys Asp Asp Ile150 155 160 165cgc gtg gtc gat gtt tgg cct ttg tgg gcg aaa acc gat ttg gtg aac 643Arg Val Val Asp Val Trp Pro Leu Trp Ala Lys Thr Asp Leu Val Asn
170 175 180ggc gtg aag gct aag tca ctg aag cgt ttg ggt gtc cgg atc act ccg 691Gly Val Lys Ala Lys Ser Leu Lys Arg Leu Gly Val Arg Ile Thr Pro
185 190 195gaa cag gtg gca cag gcg gta tgg gat gcg gtg cat ccg aaa tct cgg 739Glu Gln Val Ala Gln Ala Val Trp Asp Ala Val His Pro Lys Ser Arg
200 205 210tgg gcg aag gga aag gtg cat cac ggg gtg tca aag ttg gat aag gcg 787Trp Ala Lys Gly Lys Val His His Gly Val Ser Lys Leu Asp Lys Ala
215 220 225ctg tat ctc atg aaa tct ctg tcg cct gat cgg gta gcg atg tgt ttt 835Leu Tyr Leu Met Lys Ser Leu Ser Pro Asp Arg Val Ala Met Cys Phe230 235 240 245gcg cga cta atc gcc gga taaatgaatt gattatttta ggc 876Ala Arg Leu Ile Ala Gly
250<210>240<211>251<212>PRT<213>谷氨酸棒杆菌<400>240Met Lys Ser Ile Phe Ile Ser Gly Ala Ala Asn Gly Ile Gly Lys Ala1 5 10 15Val Ala Leu Lys Phe Leu His Glu Gly Trp Leu Val Gly Ala Tyr Asp
20 25 30Leu Ala Glu Ile Thr Tyr Ser His Pro Asn Leu Arg Trp Gly Tyr Leu
35 40 45Asn Val Arg Gln Ser Glu Ser Trp Asp Lys Ala Leu Glu Asp Phe Ala
50 55 60Thr His Thr Gly Gly Thr Ile Asp Val Val Asp Asn Asn Ala Gly Val65 70 75 80Ile Ile Glu Gly Pro Leu Gln Asp Ala Glu Glu Gly Ser Val Asp Lys
85 90 95Leu Leu Ala Ile Asn Val Asn Gly Val Thr Leu Gly Ala Arg Ala Ala
100 105 110His Pro Tyr Leu Ala Arg Thr Pro Gly Ala Gln Leu Leu Asn Met Ser
115 120 125Ser Ala Ser Ala Val Tyr Gly Gln Pro Gln Ile Ala Val Tyr Ser Ala
130 135 140Ser Lys Phe Tyr Val Ala Gly Leu Thr Glu Ala Leu Asn Leu Glu Trp145 150 155 160Arg Lys Asp Asp Ile Arg Val Val Asp Val Trp Pro Leu Trp Ala Lys
165 170 175Thr Asp Leu Val Asn Gly Val Lys Ala Lys Ser Leu Lys Arg Leu Gly
180 185 190Val Arg Ile Thr Pro Glu Gln Val Ala Gln Ala Val Trp Asp Ala Val
195 200 205His Pro Lys Ser Arg Trp Ala Lys Gly Lys Val His His Gly Val Ser
210 215 220Lys Leu Asp Lys Ala Leu Tyr Leu Met Lys Ser Leu Ser Pro Asp Arg225 230 235 240Val Ala Met Cys Phe Ala Arg Leu Ile Ala Gly
245 250<210>241<211>1140<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1117)<223>RXS01758<400>241cccccttatt cagagtgatg gtctaccgga gaagtaccca gaccaatagc atcgaccaac 60gatagcgcgc tcagaagttc tttagtgaaa gcagaaccaa atg ccc aaa tac att 115
Met Pro Lys Tyr Ile
1 5gcc atg cag gta tcc gaa tcc ggt gca ccg tta gcc gcg aat ctc gtg 163Ala Met Gln Val Ser Glu Ser Gly Ala Pro Leu Ala Ala Asn Leu Val
10 15 20caa cct gct ccg ttg aaa tcg agg gaa gtc cgc gtg gaa atc gct gct 211Gln Pro Ala Pro Leu Lys Ser Arg Glu Val Arg Val Glu Ile Ala Ala
25 30 35agt ggt gtg tgc cat gca gat att ggc acg gca gca gca tcg ggg aag 259Ser Gly Val Cys His Ala Asp Ile Gly Thr Ala Ala Ala Ser Gly Lys
40 45 50cac act gtt ttt cct gtt acc cct ggt cat gag att gca gga acc atc 307His Thr Val Phe Pro Val Thr Pro Gly His Glu Ile Ala Gly Thr Ile
55 60 65gcg gaa att ggt gaa aac gta tct cgg tgg acg gtt ggt gat cgc gtt 355Ala Glu Ile Gly Glu Asn Val Ser Arg Trp Thr Val Gly Asp Arg Val70 75 80 85gca atc ggt tgg ttt ggt ggc aat tgc ggt gac tgc gct ttt tgt cgt 403Ala Ile Gly Trp Phe Gly Gly Asn Cys Gly Asp Cys Ala Phe Cys Arg
90 95 100gca ggt gat cct gtg cat tgc aga gag cgg aag att cct ggc gtt tct 451Ala Gly Asp Pro Val His Cys Arg Glu Arg Lys Ile Pro Gly Val Ser
105 110 115tat gcg ggt ggt tgg gca cag aat att gtt gtt cca gcg gag gct ctt 499Tyr Ala Gly Gly Trp Ala Gln Asn Ile Val Val Pro Ala Glu Ala Leu
120 125 130gct gcg att cca gat ggc atg gac ttt tac gag ccc gcc ccg atg ggc 547Ala Ala Ile Pro Asp Gly Met Asp Phe Tyr Glu Pro Ala Pro Met Gly
135 140 145tgc gca ggt gtg aca aca ttc aat gcg ttg cga aac ctg aag ctg gat 595Cys Ala Gly Val Thr Thr Phe Asn Ala Leu Arg Asn Leu Lys Leu Asp150 155 160 165ccc ggt gcg gct gtc gcg gtc ttt gga atc ggc ggt tta gtg cgc cta 643Pro Gly Ala Ala Val Ala Val Phe Gly Ile Gly Gly Leu Val Arg Leu
170 175 180gct att cag ttt gct gcg aaa atg ggt tat cga acc atc acc atc gcc 691Ala Ile Gln Phe Ala Ala Lys Met Gly Tyr Arg Thr Ile Thr Ile Ala
185 190 195cgc ggt tta gag cgt gag gag cta gct agg caa ctt ggc gcc aac cac 739Arg Gly Leu Glu Arg Glu Glu Leu Ala Arg Gln Leu Gly Ala Asn His
200 205 210tac atc gat agc aat gat ctg cac cct ggc cag gcg tta ttt gaa ctt 787Tyr Ile Asp Ser Asn Asp Leu His Pro Gly Gln Ala Leu Phe Glu Leu
215 220 225ggc ggg gct gac ttg atc ttg tct act gcg tcc acc acg gag cct ctt 835Gly Gly Ala Asp Leu Ile Leu Ser Thr Ala Ser Thr Thr Glu Pro Leu230 235 240 245tcg gag ttg tct acc ggt ctt tct att ggc ggg cag cta acc att atc 883Ser Glu Leu Ser Thr Gly Leu Ser Ile Gly Gly Gln Leu Thr Ile Ile
250 255 260gga gtt gat ggg gga gat atc acc gtt tcg gca gcc caa ttg atg atg 931Gly Val Asp Gly Gly Asp Ile Thr Val Ser Ala Ala Gln Leu Met Met
265 270 275aac cgt cag atc atc aca ggt cac ctc act gga agt gcg aat gac acg 979Asn Arg Gln Ile Ile Thr Gly His Leu Thr Gly Ser Ala Asn Asp Thr
280 285 290gaa cag act atg aaa ttt gct cat ctc cat ggc gtg aaa ccg ctt att 1027Glu Gln Thr Met Lys Phe Ala His Leu His Gly Val Lys Pro Leu Ile
295 300 305gaa cgg atg cct ctc gat caa gcc aac gag gct att gca cgt att tca 1075Glu Arg Met Pro Leu Asp Gln Ala Asn Glu Ala Ile Ala Arg Ile Ser310 315 320 325gct ggt aaa cca cgt ttc cgt att gtc ttg gag ccg aat tca 1117Ala Gly Lys Pro Arg Phe Arg Ile Val Leu Glu Pro Asn Ser
330 335taatgccaac agcaagccca att 1140<210>242<211>339<212>PRT<213>谷氨酸棒杆菌<400>242Met Pro Lys Tyr Ile Ala Met Gln Val Ser Glu Ser Gly Ala Pro Leu1 5 10 15Ala Ala Asn Leu Val Gln Pro Ala Pro Leu Lys Ser Arg Glu Val Arg
20 25 30Val Glu Ile Ala Ala Ser Gly Val Cys His Ala Asp Ile Gly Thr Ala
35 40 45Ala Ala Ser Gly Lys His Thr Val Phe Pro Val Thr Pro Gly His Glu
50 55 60Ile Ala Gly Thr Ile Ala Glu Ile Gly Glu Asn Val Ser Arg Trp Thr65 70 75 80Val Gly Asp Arg Val Ala Ile Gly Trp Phe Gly Gly Asn Cys Gly Asp
85 90 95Cys Ala Phe Cys Arg Ala Gly Asp Pro Val His Cys Arg Glu Arg Lys
100 105 110Ile Pro Gly Val Ser Tyr Ala Gly Gly Trp Ala Gln Asn Ile Val Val
115 120 125Pro Ala Glu Ala Leu Ala Ala Ile Pro Asp Gly Met Asp Phe Tyr Glu
130 135 140Pro Ala Pro Met Gly Cys Ala Gly Val Thr Thr Phe Asn Ala Leu Arg145 150 155 160Asn Leu Lys Leu Asp Pro Gly Ala Ala Val Ala Val Phe Gly Ile Gly
165 170 175Gly Leu Val Arg Leu Ala Ile Gln Phe Ala Ala Lys Met Gly Tyr Arg
180 185 190Thr Ile Thr Ile Ala Arg Gly Leu Glu Arg Glu Glu Leu Ala Arg Gln
195 200 205Leu Gly Ala Asn His Tyr Ile Asp Ser Asn Asp Leu His Pro Gly Gln
210 215 220Ala Leu Phe Glu Leu Gly Gly Ala Asp Leu Ile Leu Ser Thr Ala Ser225 230 235 240Thr Thr Glu Pro Leu Ser Glu Leu Ser Thr Gly Leu Ser Ile Gly Gly
245 250 255Gln Leu Thr Ile Ile Gly Val Asp Gly Gly Asp Ile Thr Val Ser Ala
260 265 270Ala Gln Leu Met Met Asn Arg Gln Ile Ile Thr Gly His Leu Thr Gly
275 280 285Ser Ala Asn Asp Thr Glu Gln Thr Met Lys Phe Ala His Leu His Gly
290 295 300Val Lys Pro Leu Ile Glu Arg Met Pro Leu Asp Gln Ala Asn Glu Ala305 310 315 320Ile Ala Arg Ile Ser Ala Gly Lys Pro Arg Phe Arg Ile Val Leu Glu
325 330 335Pro Asn Ser<210>243<211>1665<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1642)<223>RXA02737<400>243agcacgctgc atcagtaacg gcgacatgaa atcgaattag ttcgatctta tgtggccgtt 60acacatcttt cattaaagaa aggatcgtga cactaccatc gtg agc aca aac acg 115
Val Ser Thr Asn Thr
1 5acc ccc tcc agc tgg aca aac cca ctg cgc gac ccg cag gat aaa cga 163Thr Pro Ser Ser Trp Thr Asn Pro Leu Arg Asp Pro Gln Asp Lys Arg
10 15 20ctc ccc cgc atc gct ggc cct tcc ggc atg gtg atc ttc ggt gtc act 211Leu Pro Arg Ile Ala Gly Pro Ser Gly Met Val Ile Phe Gly Val Thr
25 30 35ggc gac ttg gct cga aag aag ctg ctc ccc gcc att tat gat cta gca 259Gly Asp Leu Ala Arg Lys Lys Leu Leu Pro Ala Ile Tyr Asp Leu Ala
40 45 50aac cgc gga ttg ctg ccc cca gga ttc tcg ttg gta ggt tac ggc cgc 307Asn Arg Gly Leu Leu Pro Pro Gly Phe Ser Leu Val Gly Tyr Gly Arg
55 60 65cgc gaa tgg tcc aaa gaa gac ttt gaa aaa tac gta cgc gat gcc gca 355Arg Glu Trp Ser Lys Glu Asp Phe Glu Lys Tyr Val Arg Asp Ala Ala70 75 80 85agt gct ggt gct cgt acg gaa ttc cgt gaa aat gtt tgg gag cgc ctc 403Ser Ala Gly Ala Arg Thr Glu Phe Arg Glu Asn Val Trp Glu Arg Leu
90 95 100gcc gag ggt atg gaa ttt gtt cgc ggc aac ttt gat gat gat gca gct 451Ala Glu Gly Met Glu Phe Val Arg Gly Asn Phe Asp Asp Asp Ala Ala
105 110 115ttc gac aac ctc gct gca aca ctc aag cgc atc gac aaa acc cgc ggc 499Phe Asp Asn Leu Ala Ala Thr Leu Lys Arg Ile Asp Lys Thr Arg Gly
120 125 130acc gcc ggc aac tgg gct tac tac ctg tcc att cca cca gat tcc ttc 547Thr Ala Gly Asn Trp Ala Tyr Tyr Leu Ser Ile Pro Pro Asp Ser Phe
135 140 145aca gcg gtc tgc cac cag ctg gag cgt tcc ggc atg gct gaa tcc acc 595Thr Ala Val Cys His Gln Leu Glu Arg Ser Gly Met Ala Glu Ser Thr150 155 160 165gaa gaa gca tgg cgc cgc gtg atc atc gag aag cct ttc ggc cac aac 643Glu Glu Ala Trp Arg Arg Val Ile Ile Glu Lys Pro Phe Gly His Asn
170 175 180ctc gaa tcc gca cac gag ctc aac cag ctg gtc aac gca gtc ttc cca 691Leu Glu Ser Ala His Glu Leu Asn Gln Leu Val Asn Ala Val Phe Pro
185 190 195gaa tct tct gtg ttc cgc atc gac cac tat ttg ggc aag gaa aca gtt 739Glu Ser Ser Val Phe Arg Ile Asp His Tyr Leu Gly Lys Glu Thr Val
200 205 210caa aac atc ctg gct ctg cgt ttt gct aac cag ctg ttt gag cca ctg 787Gln Asn Ile Leu Ala Leu Arg Phe Ala Asn Gln Leu Phe Glu Pro Leu
215 220 225tgg aac tcc aac tac gtt gac cac gtc cag atc acc atg gct gaa gat 835Trp Asn Ser Asn Tyr Val Asp His Val Gln Ile Thr Met Ala Glu Asp230 235 240 245att ggc ttg ggt gga cgt gct ggt tac tac gac ggc atc ggc gca gcc 883Ile Gly Leu Gly Gly Arg Ala Gly Tyr Tyr Asp Gly Ile Gly Ala Ala
250 255 260cgc gac gtc atc cag aac cac ctg atc cag ctc ttg gct ctg gtt gcc 931Arg Asp Val Ile Gln Asn His Leu Ile Gln Leu Leu Ala Leu Val Ala
265 270 275atg gaa gaa cca att tct ttc gtg cca gcg cag ctg cag gca gaa aag 979Met Glu Glu Pro Ile Ser Phe Val Pro Ala Gln Leu Gln Ala Glu Lys
280 285 290atc aag gtg ctc tct gcg aca aag ccg tgc tac cca ttg gat aaa acc 1027Ile Lys Val Leu Ser Ala Thr Lys Pro Cys Tyr Pro Leu Asp Lys Thr
295 300 305tcc gct cgt ggt cag tac gct gcc ggt tgg cag ggc tct gag tta gtc 1075Ser Ala Arg Gly Gln Tyr Ala Ala Gly Trp Gln Gly Ser Glu Leu Val310 315 320 325aag gga ctt cgc gaa gaa gat ggc ttc aac cct gag tcc acc act gag 1123Lys Gly Leu Arg Glu Glu Asp Gly Phe Asn Pro Glu Ser Thr Thr Glu
330 335 340act ttt gcg gct tgt acc tta gag atc acg tct cgt cgc tgg gct ggt 1171Thr Phe Ala Ala Cys Thr Leu Glu Ile Thr Ser Arg Arg Trp Ala Gly
345 350 355gtg ccg ttc tac ctg cgc acc ggt aag cgt ctt ggt cgc cgt gtt act 1219Val Pro Phe Tyr Leu Arg Thr Gly Lys Arg Leu Gly Arg Arg Val Thr
360 365 370gag att gcc gtg gtg ttt aaa gac gca cca cac cag cct ttc gac ggc 1267Glu Ile Ala Val Val Phe Lys Asp Ala Pro His Gln Pro Phe Asp Gly
375 380 385gac atg act gta tcc ctt ggc caa aac gcc atc gtg att cgc gtg cag 1315Asp Met Thr Val Ser Leu Gly Gln Asn Ala Ile Val Ile Arg Val Gln390 395 400 405cct gat gaa ggt gtg ctc atc cgc ttc ggt tcc aag gtt cca ggt tct 1363Pro Asp Glu Gly Val Leu Ile Arg Phe Gly Ser Lys Val Pro Gly Ser
410 415 420gcc atg gaa gtc cgt gac gtc aac atg gac ttc tcc tac tca gaa tcc 1411Ala Met Glu Val Arg Asp Val Asn Met Asp Phe Ser Tyr Ser Glu Ser
425 430 435ttc act gaa gaa tca cct gaa gca tac gag cgc ctc att ttg gat gcg 1459Phe Thr Glu Glu Ser Pro Glu Ala Tyr Glu Arg Leu Ile Leu Asp Ala
440 445 450ctg tta gat gaa tcc agc ctc ttc cct acc aac gag gaa gtg gaa ctg 1507Leu Leu Asp Glu Ser Ser Leu Phe Pro Thr Asn Glu Glu Val Glu Leu
455 460 465agc tgg aag att ctg gat cca att ctt gaa gca tgg gat gcc gat gga 1555Ser Trp Lys Ile Leu Asp Pro Ile Leu Glu Ala Trp Asp Ala Asp Gly470 475 480 485gaa cca gag gat tac cca gcg ggt acg tgg ggt cca aag agc gct gat 1603Glu Pro Glu Asp Tyr Pro Ala Gly Thr Trp Gly Pro Lys Ser Ala Asp
490 495 500gaa atg ctt tcc cgc aac ggt cac acc tgg cgc agg cca taatttaggg 1652Glu Met Leu Ser Arg Asn Gly His Thr Trp Arg Arg Pro
505 510gcaaaaaatg atc 1665<210>244<211>514<212>PRT<213>谷氨酸棒杆菌<400>244Val Ser Thr Asn Thr Thr Pro Ser Ser Trp Thr Asn Pro Leu Arg Asp1 5 10 15Pro Gln Asp Lys Arg Leu Pro Arg Ile Ala Gly Pro Ser Gly Met Val
20 25 30Ile Phe Gly Val Thr Gly Asp Leu Ala Arg Lys Lys Leu Leu Pro Ala
35 40 45Ile Tyr Asp Leu Ala Asn Arg Gly Leu Leu Pro Pro Gly Phe Ser Leu
50 55 60Val Gly Tyr Gly Arg Arg Glu Trp Ser Lys Glu Asp Phe Glu Lys Tyr65 70 75 80Val Arg Asp Ala Ala Ser Ala Gly Ala Arg Thr Glu Phe Arg Glu Asn
85 90 95Val Trp Glu Arg Leu Ala Glu Gly Met Glu Phe Val Arg Gly Asn Phe
100 105 110Asp Asp Asp Ala Ala Phe Asp Asn Leu Ala Ala Thr Leu Lys Arg Ile
115 120 125Asp Lys Thr Arg Gly Thr Ala Gly Asn Trp Ala Tyr Tyr Leu Ser Ile
130 135 140Pro Pro Asp Ser Phe Thr Ala Val Cys His Gln Leu Glu Arg Ser Gly145 150 155 160Met Ala Glu Ser Thr Glu Glu Ala Trp Arg Arg Val Ile Ile Glu Lys
165 170 175Pro Phe Gly His Asn Leu Glu Ser Ala His Glu Leu Asn Gln Leu Val
180 185 190Asn Ala Val Phe Pro Glu Ser Ser Val Phe Arg Ile Asp His Tyr Leu
195 200 205Gly Lys Glu Thr Val Gln Asn Ile Leu Ala Leu Arg Phe Ala Asn Gln
210 215 220Leu Phe Glu Pro Leu Trp Asn Ser Asn Tyr Val Asp His Val Gln Ile225 230 235 240Thr Met Ala Glu Asp Ile Gly Leu Gly Gly Arg Ala Gly Tyr Tyr Asp
245 250 255Gly Ile Gly Ala Ala Arg Asp Val Ile Gln Asn His Leu Ile Gln Leu
260 265 270Leu Ala Leu Val Ala Met Glu Glu Pro Ile Ser Phe Val Pro Ala Gln
275 280 285Leu Gln Ala Glu Lys Ile Lys Val Leu Ser Ala Thr Lys Pro Cys Tyr
290 295 300Pro Leu Asp Lys Thr Ser Ala Arg Gly Gln Tyr Ala Ala Gly Trp Gln305 310 315 320Gly Ser Glu Leu Val Lys Gly Leu Arg Glu Glu Asp Gly Phe Asn Pro
325 330 335Glu Ser Thr Thr Glu Thr Phe Ala Ala Cys Thr Leu Glu Ile Thr Ser
340 345 350Arg Arg Trp Ala Gly Val Pro Phe Tyr Leu Arg Thr Gly Lys Arg Leu
355 360 365Gly Arg Arg Val Thr Glu Ile Ala Val Val Phe Lys Asp Ala Pro His
370 375 380Gln Pro Phe Asp Gly Asp Met Thr Val Ser Leu Gly Gln Asn Ala Ile385 390 395 400Val Ile Arg Val Gln Pro Asp Glu Gly Val Leu Ile Arg Phe Gly Ser
405 410 415lys Val Pro Gly Ser Ala Met Glu Val Arg Asp Val Asn Met Asp Phe
420 425 430Ser Tyr Ser Glu Ser Phe Thr Glu Glu Ser Pro Glu Ala Tyr Glu Arg
435 440 445Leu Ile Leu Asp Ala Leu Leu Asp Glu Ser Ser Leu Phe Pro Thr Asn
450 455 460Glu Glu Val Glu Leu Ser Trp Lys Ile Leu Asp Pro Ile Leu Glu Ala465 470 475 480Trp Asp Ala Asp Gly Glu Pro Glu Asp Tyr Pro Ala Gly Thr Trp Gly
485 490 495Pro Lys Ser Ala Asp Glu Met Leu Ser Arg Asn Gly His Thr Trp Arg
500 505 510Arg Pro<210>245<211>1203<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1180)<223>RXA02738<400>245ttgttgttaa tcggtacaaa gggtcttaag cacatccctt acttgcctgc tctccttgag 60cacagttcaa gaacaattct tttaaggaaa atttagtttc atg tct cac att gat 115
Met Ser His Ile Asp
1 5gat ctt gca cag ctc ggc act tcc act tgg ctc gac gac ctc tcc cgc 163Asp Leu Ala Gln Leu Gly Thr Ser Thr Trp Leu Asp Asp Leu Ser Arg
10 15 20gag cgc att act tcc ggc aat ctc agc cag gtt att gag gaa aag tct 211Glu Arg Ile Thr Ser Gly Asn Leu Ser Gln Val Ile Glu Glu Lys Ser
25 30 35gta gtc ggt gtc acc acc aac cca gct att ttc gca gca gca atg tcc 259Val Val Gly Val Thr Thr Asn Pro Ala Ile Phe Ala Ala Ala Met Ser
40 45 50aag ggc gat tcc tac gac gct cag atc gca gag ctc aag gcc gct ggc 307Lys Gly Asp Ser Tyr Asp Ala Gln Ile Ala Glu Leu Lys Ala Ala Gly
55 60 65gca tct gtt gac cag gct gtt tac gcc atg agc atc gac gac gtt cgc 355Ala Ser Val Asp Gln Ala Val Tyr Ala Met Ser Ile Asp Asp Val Arg70 75 80 85aat gct tgt gat ctg ttc acc ggc atc ttc gag tcc tcc aac ggc tac 403Asn Ala Cys Asp Leu Phe Thr Gly Ile Phe Glu Ser Ser Asn Gly Tyr
90 95 100gac ggc cgc gtg tcc atc gag gtt gac cca cgt atc tct gct gac cgc 451Asp Gly Arg Val Ser Ile Glu Val Asp Pro Arg Ile Ser Ala Asp Arg
105 110 115gac gca acc ctg gct cag gcc aag gag ctg tgg gca aag gtt gat cgt 499Asp Ala Thr Leu Ala Gln Ala Lys Glu Leu Trp Ala Lys Val Asp Arg
120 125 130cca aac gtc atg atc aag atc cct gca acc cca ggt tct ttg cca gca 547Pro Asn Val Met Ile Lys Ile Pro Ala Thr Pro Gly Ser Leu Pro Ala
135 140 145atc acc gac gct ttg gct gag ggc atc agc gtt aac gtc acc ttg atc 595Ile Thr Asp Ala Leu Ala Glu Gly Ile Ser Val Asn Val Thr Leu Ile150 155 160 165ttc tcc gtt gct cgc tac cgc gag gtc atc gct gcg ttc atc gag ggc 643Phe Ser Val Ala Arg Tyr Arg Glu Val Ile Ala Ala Phe Ile Glu Gly
170 175 180atc aag cag gct gct gca aac ggc cac gac gtc tcc aag atc cac tct 691Ile Lys Gln Ala Ala Ala Asn Gly His Asp Val Ser Lys Ile His Ser
185 190 195gtg gct tcc ttc ttc gtc tcc cgc gtc gac gtt gag atc gac aag cgc 739Val Ala Ser Phe Phe Val Ser Arg Val Asp Val Glu Ile Asp Lys Arg
200 205 210ctc gag gca atc gga tcc gat gag gct ttg gct ctg cgc ggc aag gca 787Leu Glu Ala Ile Gly Ser Asp Glu Ala Leu Ala Leu Arg Gly Lys Ala
215 220 225ggc gtt gcc aac gct cag cgc gct tac gct gtg tac aag gag ctt ttc 835Gly Val Ala Asn Ala Gln Arg Ala Tyr Ala Val Tyr Lys Glu Leu Phe230 235 240 245gac gcc gcc gag ctg cct gaa ggt gcc aac act cag cgc cca ctg tgg 883Asp Ala Ala Glu Leu Pro Glu Gly Ala Asn Thr Gln Arg Pro Leu Trp
250 255 260gca tcc acc ggc gtg aag aac cct gcg tac gct gca act ctt tac gtt 931Ala Ser Thr Gly Val Lys Asn Pro Ala Tyr Ala Ala Thr Leu Tyr Val
265 270 275tcc gag ctg gct ggt cca aac acc gtc aac acc atg cca gaa ggc acc 979Ser Glu Leu Ala Gly Pro Asn Thr Val Asn Thr Met Pro Glu Gly Thr
280 285 290atc gac gcg gtt ctg gag cag ggc aac ctg cac ggt gac acc ctg tcc 1027Ile Asp Ala Val Leu Glu Gln Gly Asn Leu His Gly Asp Thr Leu Ser
295 300 305aac tcc gcg gca gaa gct gac gct gtg ttc tcc cag ctt gag gct ctg 1075Asn Ser Ala Ala Glu Ala Asp Ala Val Phe Ser Gln Leu Glu Ala Leu310 315 320 325ggc gtt gac ttg gca gat gtc ttc cag gtc ctg gag acc gag ggt gtg 1123Gly Val Asp Leu Ala Asp Val Phe Gln Val Leu Glu Thr Glu Gly Val
330 335 340gac aag ttc gtt gct tct tgg agc gaa ctg ctt gag tcc atg gaa gct 1171Asp Lys Phe Val Ala Ser Trp Ser Glu Leu Leu Glu Ser Met Glu Ala
345 350 355cgc ctg aag tagaatcagc acgctgcatc agt 1203Arg Leu Lys
360<210>246<211>360<212>PRT<213>谷氨酸棒杆菌<400>246Met Ser His Ile Asp Asp Leu Ala Gln Leu Gly Thr Ser Thr Trp Leu1 5 10 15Asp Asp Leu Ser Arg Glu Arg Ile Thr Ser Gly Asn Leu Ser Gln Val
20 25 30Ile Glu Glu Lys Ser Val Val Gly Val Thr Thr Asn Pro Ala Ile Phe
35 40 45Ala Ala Ala Met Ser Lys Gly Asp Ser Tyr Asp Ala Gln Ile Ala Glu
50 55 60Leu Lys Ala Ala Gly Ala Ser Val Asp Gln Ala Val Tyr Ala Met Ser65 70 75 80Ile Asp Asp Val Arg Asn Ala Cys Asp Leu Phe Thr Gly Ile Phe Glu
85 90 95Ser Ser Asn Gly Tyr Asp Gly Arg Val Ser Ile Glu Val Asp Pro Arg
100 105 110Ile Ser Ala Asp Arg Asp Ala Thr Leu Ala Gln Ala Lys Glu Leu Trp
115 120 125Ala Lys Val Asp Arg Pro Asn Val Met Ile Lys Ile Pro Ala Thr Pro
130 135 140Gly Ser Leu Pro Ala Ile Thr Asp Ala Leu Ala Glu Gly Ile Ser Val145 150 155 160Asn Val Thr Leu Ile Phe Ser Val Ala Arg Tyr Arg Glu Val Ile Ala
165 170 175Ala Phe Ile Glu Gly Ile Lys Gln Ala Ala Ala Asn Gly His Asp Val
180 185 190Ser Lys Ile His Ser Val Ala Ser Phe Phe Val Ser Arg Val Asp Val
195 200 205Glu Ile Asp Lys Arg Leu Glu Ala Ile Gly Ser Asp Glu Ala Leu Ala
210 215 220Leu Arg Gly Lys Ala Gly Val Ala Asn Ala Gln Arg Ala Tyr Ala Val225 230 235 240Tyr Lys Glu Leu Phe Asp Ala Ala Glu Leu Pro Glu Gly Ala Asn Thr
245 250 255Gln Arg Pro Leu Trp Ala Ser Thr Gly Val Lys Asn Pro Ala Tyr Ala
260 265 270Ala Thr Leu Tyr Val Ser Glu Leu Ala Gly Pro Asn Thr Val Asn Thr
275 280 285Met Pro Glu Gly Thr Ile Asp Ala Val Leu Glu Gln Gly Asn Leu His
290 295 300Gly Asp Thr Leu Ser Asn Ser Ala Ala Glu Ala Asp Ala Val Phe Ser305 310 315 320Gln Leu Glu Ala Leu Gly Val Asp Leu Ala Asp Val Phe Gln Val Leu
325 330 335Glu Thr Glu Gly Val Asp Lys Phe Val Ala Ser Trp Ser Glu Leu Leu
340 345 350Glu Ser Met Glu Ala Arg Leu Lys
355 360<210>247<211>2223<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2200)<223>RXA02739<400>247cctttgccaa atttgaacca attaacctaa gtcgtagatc tgatcatcgg atctaacgaa 60aacgaaccaa aactttggtc ccggtttaac ccaggaagga ttg acc acc ttg acg 115
Leu Thr Thr Leu Thr
1 5ctg tca cct gaa ctt cag gcg ctc act gta cgc aat tac ccc tct gat 163Leu Ser Pro Glu Leu Gln Ala Leu Thr Val Arg Asn Tyr Pro Ser Asp
10 15 20tgg tcc gat gtg gac acc aag gct gta gac act gtt cgt gtc ctc gct 211Trp Ser Asp Val Asp Thr Lys Ala Val Asp Thr Val Arg Val Leu Ala
25 30 35gca gac gct gta gaa aac tgt ggc tcc ggc cac cca ggc acc gca atg 259Ala Asp Ala Val Glu Asn Cys Gly Ser Gly His Pro Gly Thr Ala Met
40 45 50agc ctg gct ccc ctt gca tac acc ttg tac cag cgg gtt atg aac gta 307Ser Leu Ala Pro Leu Ala Tyr Thr Leu Tyr Gln Arg Val Met Asn Val
55 60 65gat cca cag gac acc aac tgg gca ggc cgt gac cgc ttc gtt ctt tct 355Asp Pro Gln Asp Thr Asn Trp Ala Gly Arg Asp Arg Phe Val Leu Ser70 75 80 85tgt ggc cac tcc tct ttg acc cag tac atc cag ctt tac ttg ggt gga 403Cys Gly His Ser Ser Leu Thr Gln Tyr Ile Gln Leu Tyr Leu Gly Gly
90 95 100ttc ggc ctt gag atg gat gac ctg aag gct ctg cgc acc tgg gat tcc 451Phe Gly Leu Glu Met Asp Asp Leu Lys Ala Leu Arg Thr Trp Asp Ser
105 110 115ttg acc cca gga cac cct gag tac cgc cac acc aag ggc gtt gag atc 499Leu Thr Pro Gly His Pro Glu Tyr Arg His Thr Lys Gly Val Glu Ile
120 125 130acc act ggc cct ctt ggc cag ggt ctt gca tct gca gtt ggt atg gcc 547Thr Thr Gly Pro Leu Gly Gln Gly Leu Ala Ser Ala Val Gly Met Ala
135 140 145atg gct gct cgt cgt gag cgt ggc cta ttc gac cca acc gct gct gag 595Met Ala Ala Arg Arg Glu Arg Gly Leu Phe Asp Pro Thr Ala Ala Glu150 155 160 165ggc gaa tcc cca ttc gac cac cac atc tac gtc att gct tct gat ggt 643Gly Glu Ser Pro Phe Asp His His Ile Tyr Val Ile Ala Ser Asp Gly
170 175 180gac ctg cag gaa ggt gtc acc tct gag gca tcc tcc atc gct ggc acc 691Asp Leu Gln Glu Gly Val Thr Ser Glu Ala Ser Ser Ile Ala Gly Thr
185 190 195cag cag ctg ggc aac ctc atc gtg ttc tgg gat gac aac cgc atc tcc 739Gln Gln Leu Gly Asn Leu Ile Val Phe Trp Asp Asp Asn Arg Ile Ser
200 205 210atc gaa gac aac act gag atc gct ttc aac gag gac gtt gtt gct cgt 787Ile Glu Asp Asn Thr Glu Ile Ala Phe Asn Glu Asp Val Val Ala Arg
215 220 225tac aag gct tac ggc tgg cag acc att gag gtt gag gct ggc gag gac 835Tyr Lys Ala Tyr Gly Trp Gln Thr Ile Glu Val Glu Ala Gly Glu Asp230 235 240 245gtt gca gca atc gaa gct gca gtg gct gag gct aag aag gac acc aag 883Val Ala Ala Ile Glu Ala Ala Val Ala Glu Ala Lys Lys Asp Thr Lys
250 255 260cga cct acc ttc atc cgc gtt cgc acc atc atc ggc ttc cca gct cca 931Arg Pro Thr Phe Ile Arg Val Arg Thr Ile Ile Gly Phe Pro Ala Pro
265 270 275act atg atg aac acc ggt gct gtg cac ggt gct gct ctt ggc gca gct 979Thr Met Met Asn Thr Gly Ala Val His Gly Ala Ala Leu Gly Ala Ala
280 285 290gag gtt gca gca acc aag act gag ctt gga ttc gat cct gag gct cac 1027Glu Val Ala Ala Thr Lys Thr Glu Leu Gly Phe Asp Pro Glu Ala His
295 300 305ttc gcg atc gac gat gag gtt atc gct cac acc cgc tcc ctc gca gag 1075Phe Ala Ile Asp Asp Glu Val Ile Ala His Thr Arg Ser Leu Ala Glu310 315 320 325cgc gct gca cag aag aag gct gca tgg cag gte aag ttc gat gag tgg 1123Arg Ala Ala Gln Lys Lys Ala Ala Trp Gln Val Lys Phe Asp Glu Trp
330 335 340gca gct gcc aac cct gag aac aag gct ctg ttc gat cgc ctg aac tcc 1171Ala Ala Ala Asn Pro Glu Asn Lys Ala Leu Phe Asp Arg Leu Asn Ser
345 350 355cgt gag ctt cca gcg ggc tac gct gac gag ctc cca aca tgg gat gca 1219Arg Glu Leu Pro Ala Gly Tyr Ala Asp Glu Leu Pro Thr Trp Asp Ala
360 365 370gat gag aag ggc gtc gca act cgt aag gct tcc gag gct gca ctt cag 1267Asp Glu Lys Gly Val Ala Thr Arg Lys Ala Ser Glu Ala Ala Leu Gln
375 380 385gca ctg ggc aag acc ctt cct gag ctg tgg ggc ggt tcc gct gac ctc 1315Ala Leu Gly Lys Thr Leu Pro Glu Leu Trp Gly Gly Ser Ala Asp Leu390 395 400 405gca ggt tcc aac aac acc gtg atc aag ggc tcc cct tcc ttc ggc cct 1363Ala Gly Ser Asn Asn Thr Val Ile Lys Gly Ser Pro Ser Phe Gly Pro
410 415 420gag tcc atc tcc acc gag acc tgg tct gct gag cct tac ggc cgt aac 1411Glu Ser Ile Ser Thr Glu Thr Trp Ser Ala Glu Pro Tyr Gly Arg Asn
425 430 435ctg cac ttc ggt atc cgt gag cac gct atg gga tcc atc ctc aac ggc 1459Leu His Phe Gly Ile Arg Glu His Ala Met Gly Ser Ile Leu Asn Gly
440 445 450att tcc ctc cac ggt ggc acc cgc cca tac ggc gga acc ttc ctc atc 1507Ile Ser Leu His Gly Gly Thr Arg Pro Tyr Gly Gly Thr Phe Leu Ile
455 460 465ttc tcc gac tac atg cgt cct gca gtt cgt ctt gca gct ctc atg gag 1555Phe Ser Asp Tyr Met Arg Pro Ala Val Arg Leu Ala Ala Leu Met Glu470 475 480 485acc gac gct tac tac gtc tgg acc cac gac tcc atc ggt ctg ggc gaa 1603Thr Asp Ala Tyr Tyr Val Trp Thr His Asp Ser Ils Gly Leu Gly Glu
490 495 500gat ggc cca acc cac cag cct gtt gaa acc ttg gct gca ctg cgc gcc 1651Asp Gly Pro Thr His Gln Pro Val Glu Thr Leu Ala Ala Leu Arg Ala
505 510 515atc cca ggt ctg tcc gtc ctg cgt cct gca gat gcg aac gag acc gcc 1699Ile Pro Gly Leu Ser Val Leu Arg Pro Ala Asp Ala Asn Glu Thr Ala
520 525 530cag gct tgg gct gca gca ctt gag tac aag gaa ggc cct aag ggt ctt 1747Gln Ala Trp Ala Ala Ala Leu Glu Tyr Lys Glu Gly Pro Lys Gly Leu
535 540 545gca ctg acc cgc cag aac gtt cct gtt ctg gaa ggc acc aag gag aag 1795Ala Leu Thr Arg Gln Asn Val Pro Val Leu Glu Gly Thr Lys Glu Lys550 555 560 565gct gct gaa ggc gtt cgc cgc ggt ggc tac gtc ctg gtt gag ggt tcc 1843Ala Ala Glu Gly Val Arg Arg Gly Gly Tyr Val Leu Val Glu Gly Ser
570 575 580aag gaa acc cca gat gtg atc ctc atg ggc tcc ggc tcc gag gtt cag 1891Lys Glu Thr Pro Asp Val Ile Leu Met Gly Ser Gly Ser Glu Val Gln
585 590 595ctt gca gtt aac gct gcg aag gct ctg gaa gct gag ggc gtt gca gct 1939Leu Ala Val Asn Ala Ala Lys Ala Leu Glu Ala Glu Gly Val Ala Ala
600 605 610cgc gtt gtt tcc gtt cct tgc atg gat tgg ttc cag gag cag gac gca 1987Arg Val Val Ser Val Pro Cys Met Asp Trp Phe Gln Glu Gln Asp Ala
615 620 625gag tac atc gag tcc gtt ctg cct gca gct gtg acc gct cgt gtg tct 2035Glu Tyr Ile Glu Ser Val Leu Pro Ala Ala Val Thr Ala Arg Val Ser630 635 640 645gtt gaa gct ggc atc gca atg cct tgg tac cgc ttc ttg ggc acc cag 2083Val Glu Ala Gly Ile Ala Met Pro Trp Tyr Arg Phe Leu Gly Thr Gln
650 655 660ggc cgt gct gtc tcc ctt gag cac ttc ggt gct tct gcg gat tac cag 2131Gly Arg Ala Val Ser Leu Glu His Phe Gly Ala Ser Ala Asp Tyr Gln
665 670 675acc ctg ttt gag aag ttc ggc atc acc acc gat gca gtc gtg gca gcg 2179Thr Leu Phe Glu Lys Phe Gly Ile Thr Thr Asp Ala Val Val Ala Ala
680 685 690gcc aag gac tcc att aac ggt taattgccct gctgttttta gct 2223Ala Lys Asp Ser Ile Asn Gly
695 700<210>248<211>700<212>PRT<213>谷氨酸棒杆菌<400>248Leu Thr Thr Leu Thr Leu Ser Pro Glu Leu Gln Ala Leu Thr Val Arg1 5 10 15Asn Tyr Pro Ser Asp Trp Ser Asp Val Asp Thr Lys Ala Val Asp Thr
20 25 30Val Arg Val Leu Ala Ala Asp Ala Val Glu Asn Cys Gly Ser Gly His
35 40 45Pro Gly Thr Ala Met Ser Leu Ala Pro Leu Ala Tyr Thr Leu Tyr Gln
50 55 60Arg Val Met Asn Val Asp Pro Gln Asp Thr Asn Trp Ala Gly Arg Asp65 70 75 80Arg Phe Val Leu Ser Cys Gly His Ser Ser Leu Thr Gln Tyr Ile Gln
85 90 95Leu Tyr Leu Gly Gly Phe Gly Leu Glu Met Asp Asp Leu Lys Ala Leu
100 105 110Arg Thr Trp Asp Ser Leu Thr Pro Gly His Pro Glu Tyr Arg His Thr
115 120 125Lys Gly Val Glu Ile Thr Thr Gly Pro Leu Gly Gln Gly Leu Ala Ser
130 135 140Ala Val Gly Met Ala Met Ala Ala Arg Arg Glu Arg Gly Leu Phe Asp145 150 155 160Pro Thr Ala Ala Glu Gly Glu Ser Pro Phe Asp His His Ile Tyr Val
165 170 175Ile Ala Ser Asp Gly Asp Leu Gln Glu Gly Val Thr Ser Glu Ala Ser
180 185 190Ser Ile Ala Gly Thr Gln Gln Leu Gly Asn Leu Ile Val Phe Trp Asp
195 200 205Asp Asn Arg Ile Ser Ile Glu Asp Asn Thr Glu Ile Ala Phe Asn Glu
210 215 220Asp Val Val Ala Arg Tyr Lys Ala Tyr Gly Trp Gln Thr Ile Glu Val225 230 235 240Glu Ala Gly Glu Asp Val Ala Ala Ile Glu Ala Ala Val Ala Glu Ala
245 250 255Lys Lys Asp Thr Lys Arg Pro Thr Phe Ile Arg Val Arg Thr Ile Ile
260 265 270Gly Phe Pro Ala Pro Thr Met Met Asn Thr Gly Ala Val His Gly Ala
275 280 285Ala Leu Gly Ala Ala Glu Val Ala Ala Thr Lys Thr Glu Leu Gly Phe
290 295 300Asp Pro Glu Ala His Phe Ala Ile Asp Asp Glu Val Ile Ala His Thr305 310 315 320Arg Ser Leu Ala Glu Arg Ala Ala Gln Lys Lys Ala Ala Trp Gln Val
325 330 335Lys Phe Asp Glu Trp Ala Ala Ala Asn Pro Glu Asn Lys Ala Leu Phe
340 345 350Asp Arg Leu Asn Ser Arg Glu Leu Pro Ala Gly Tyr Ala Asp Glu Leu
355 360 365Pro Thr Trp Asp Ala Asp Glu Lys Gly Val Ala Thr Arg Lys Ala Ser
370 375 380Glu Ala Ala Leu Gln Ala Leu Gly Lys Thr Leu Pro Glu Leu Trp Gly385 390 395 400Gly Ser Ala Asp Leu Ala Gly Ser Asn Asn Thr Val Ile Lys Gly Ser
405 410 415Pro Ser Phe Gly Pro Glu Ser Ile Ser Thr Glu Thr Trp Ser Ala Glu
420 425 430Pro Tyr Gly Arg Asn Leu His Phe Gly Ile Arg Glu His Ala Met Gly
435 440 445Ser Ile Leu Asn Gly Ile Ser Leu His Gly Gly Thr Arg Pro Tyr Gly
450 455 460Gly Thr Phe Leu Ile Phe Ser Asp Tyr Met Arg Pro Ala Val Arg Leu465 470 475 480Ala Ala Leu Met Glu Thr Asp Ala Tyr Tyr Val Trp Thr His Asp Ser
485 490 495Ile Gly Leu Gly Glu Asp Gly Pro Thr His Gln Pro Val Glu Thr Leu
500 505 510Ala Ala Leu Arg Ala Ile Pro Gly Leu Ser Val Leu Arg Pro Ala Asp
515 520 525Ala Asn Glu Thr Ala Gln Ala Trp Ala Ala Ala Leu Glu Tyr Lys Glu
530 535 540Gly Pro Lys Gly Leu Ala Leu Thr Arg Gln Asn Val Pro Val Leu Glu545 550 555 560Gly Thr Lys Glu Lys Ala Ala Glu Gly Val Arg Arg Gly Gly Tyr Val
565 570 575Leu Val Glu Gly Ser Lys Glu Thr Pro Asp Val Ile Leu Met Gly Ser
580 585 590Gly Ser Glu Val Gln Leu Ala Val Asn Ala Ala Lys Ala Leu Glu Ala
595 600 605Glu Gly Val Ala Ala Arg Val Val Ser Val Pro Cys Met Asp Trp Phe
610 615 620Gln Glu Gln Asp Ala Glu Tyr Ile Glu Ser Val Leu Pro Ala Ala Val625 630 635 640Thr Ala Arg Val Ser Val Glu Ala Gly Ile Ala Met Pro Trp Tyr Arg
645 650 655Phe Leu Gly Thr Gln Gly Arg Ala Val Ser Leu Glu His Phe Gly Ala
660 665 670Ser Ala Asp Tyr Gln Thr Leu Phe Glu Lys Phe Gly Ile Thr Thr Asp
675 680 685Ala Val Val Ala Ala Ala Lys Asp Ser Ile Asn Gly
690 695 700<210>249<211>793<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(48)..(770)<223>RXA00965<400>249agattgcggg cctcggcttc attgaaaaca agacggtgtt tgaataa atg aca act 56
Met Thr Thr
1ttc cac gat ctt ccg ctg gag gag cgg ctg aca ctg gcc agg ttg ggc 104Phe His Asp Leu Pro Leu Glu Glu Arg Leu Thr Leu Ala Arg Leu Gly
5 10 15aca tcc cac tac tcc cgt cag ctc tcc ctc gtg gac aac gct gag ttc 152Thr Ser His Tyr Ser Arg Gln Leu Ser Leu Val Asp Asn Ala Glu Phe20 25 30 35ggc gag cat tcc ctg cta gaa ggg tgg act cgt tcc cac ctc att gcc 200Gly Glu His Ser Leu Leu Glu Gly Trp Thr Arg Ser His Leu Ile Ala
40 45 50cac gtg gca tac aac gcc atc gca ctg tgc aac ctc atg cac tgg gca 248His Val Ala Tyr Asn Ala Ile Ala Leu Cys Asn Leu Met His Trp Ala
55 60 65aat act ggt gag gaa acc cca atg tac gtg tcg cca gaa gcg cgc aac 296Asn Thr Gly Glu Glu Thr Pro Met Tyr Val Ser Pro Glu Ala Arg Asn
70 75 80gag gaa att gcc tac ggt tcc acg ctc aat ccc gat gcg ttg cgt aac 344Glu Glu Ile Ala Tyr Gly Ser Thr Leu Asn Pro Asp Ala Leu Arg Asn
85 90 95ctg cat gaa cac tcc gtc gca cgc ctg gac gtg gct tgg cgt gaa acg 392Leu His Glu His Ser Val Ala Arg Leu Asp Val Ala Trp Arg Glu Thr100 105 110 115tct gaa gat gct tgg tca cac gag gtt ctg aca gct cag gga cgc act 440Ser Glu Asp Ala Trp Ser His Glu Val Leu Thr Ala Gln Gly Arg Thr
120 125 130gtc cca gct agt gaa aca ttg tgg atg cgt tcc cgc gaa gtc tgg atc 488Val Pro Ala Ser Glu Thr Leu Trp Met Arg Ser Arg Glu Val Trp Ile
135 140 145cac gca gtt gac ctc ggt gca gtg gca acc ttt ggc gac atc cca gag 536His Ala Val Asp Leu Gly Ala Val Ala Thr Phe Gly Asp Ile Pro Glu
150 155 160gtc atc ctg cgc acc tta gct gca gaa atc aca caa aag tgg aca agc 584Val Ile Leu Arg Thr Leu Ala Ala Glu Ile Thr Gln Lys Trp Thr Ser
165 170 175caa gga gcc ggc gag gga ctt gtg ctt ctc gac gag ccc tcc agc act 632Gln Gly Ala Gly Glu Gly Leu Val Leu Leu Asp Glu Pro Ser Ser Thr180 185 190 195cgc tac ccc gcc gcc cca ggg cag gac gag gta gta gtg tcc ggt agc 680Arg Tyr Pro Ala Ala Pro Gly Gln Asp Glu Val Val Val Ser Gly Ser
200 205 210ctt gca ggc att gtt cgc tac gcc gct ggc cgc ggt tcc gat gga gtc 728Leu Ala Gly Ile Val Arg Tyr Ala Ala Gly Arg Gly Ser Asp Gly Val
215 220 225act tct tcc act gga gag gtt cca gag cca ccg cgc tgg ctg 770Thr Ser Ser Thr Gly Glu Val Pro Glu Pro Pro Arg Trp Leu
230 235 240tagtttccac acattcttaa atg 793<210>250<211>241<212>PRT<213>谷氨酸棒杆菌<400>250Met Thr Thr Phe His Asp Leu Pro Leu Glu Glu Arg Leu Thr Leu Ala1 5 10 15Arg Leu Gly Thr Ser His Tyr Ser Arg Gln Leu Ser Leu Val Asp Asn
20 25 30Ala Glu Phe Gly Glu His Ser Leu Leu Glu Gly Trp Thr Arg Ser His
35 40 45Leu Ile Ala His Val Ala Tyr Asn Ala Ile Ala Leu Cys Asn Leu Met
50 55 60His Trp Ala Asn Thr Gly Glu Glu Thr Pro Met Tyr Val Ser Pro Glu65 70 75 80Ala Arg Asn Glu Glu Ile Ala Tyr Gly Ser Thr Leu Asn Pro Asp Ala
85 90 95Leu Arg Asn Leu His Glu His Ser Val Ala Arg Leu Asp Val Ala Trp
100 105 110Arg Glu Thr Ser Glu Asp Ala Trp Ser His Glu Val Leu Thr Ala Gln
115 120 125Gly Arg Thr Val Pro Ala Ser Glu Thr Leu Trp Met Arg Ser Arg Glu
130 135 140Val Trp Ile His Ala Val Asp Leu Gly Ala Val Ala Thr Phe Gly Asp145 150 155 160Ile Pro Glu Val Ile Leu Arg Thr Leu Ala Ala Glu Ile Thr Gln Lys
165 170 175Trp Thr Ser Gln Gly Ala Gly Glu Gly Leu Val Leu Leu Asp Glu Pro
180 185 190Ser Ser Thr Arg Tyr Pro Ala Ala Pro Gly Gln Asp Glu Val Val Val
195 200 205Ser Gly Ser Leu Ala Gly Ile Val Arg Tyr Ala Ala Gly Arg Gly Ser
210 215 220Asp Gly Val Thr Ser Ser Thr Gly Glu Val Pro Glu Pro Pro Arg Trp225 230 235 240Leu<210>251<211>1575<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1552)<223>RXN00999<400>251cctcctgtga cctggtaaaa tcgccactac ccccaaatgg tcacaccttt taggccgatt 60ttgctgacac cgggctatgc cgtcaagtac gatcaataac atg act aat gga gat 115
Met Thr Asn Gly Asp
1 5aat ctc gca cag atc ggc gtt gta ggc cta gca gta atg ggc tca aac 163Asn Leu Ala Gln Ile Gly Val Val Gly Leu Ala Val Met Gly Ser Asn
10 15 20ctc gcc cgc aac ttc gcc cgc aac ggc aac act gtc gct gtc tac aac 211Leu Ala Arg Asn Phe Ala Arg Asn Gly Asn Thr Val Ala Val Tyr Asn
25 30 35cgc agc act gac aaa acc gac aag ctc atc gcc gat cac ggc tcc gaa 259Arg Ser Thr Asp Lys Thr Asp Lys Leu Ile Ala Asp His Gly Ser Glu
40 45 50ggc aac ttc atc cct tct gca acc gtc gaa gag ttc gta gca tcc ctg 307Gly Asn Phe Ile Pro Ser Ala Thr Val Glu Glu Phe Val Ala Ser Leu
55 60 65gaa aag cca cgc cgc gcc atc atc atg gtt cag gct ggt aac gcc acc 355Glu Lys Pro Arg Arg Ala Ile Ile Met Val Gln Ala Gly Asn Ala Thr70 75 80 85gac gca gtc atc aac cag ctg gca gat gcc atg gac gaa ggc gac atc 403Asp Ala Val Ile Asn Gln Leu Ala Asp Ala Met Asp Glu Gly Asp Ile
90 95 100atc atc gac ggc ggc aac gcc ctc tac acc gac acc att cgt cgc gag 451Ile Ile Asp Gly Gly Asn Ala Leu Tyr Thr Asp Thr Ile Arg Arg Glu
105 110 115aag gaa atc tcc gca cgc ggt ctc cac ttc gtc ggt gct ggt atc tcc 499Lys Glu Ile Ser Ala Arg Gly Leu His Phe Val Gly Ala Gly Ile Ser
120 125 130ggc ggc gaa gaa ggc gca ctc aac ggc cca tcc atc atg cct ggt ggc 547Gly Gly Glu Glu Gly Ala Leu Asn Gly Pro Ser Ile Met Pro Gly Gly
135 140 145cca gca aag tcc tac gag tcc ctc gga cca ctg ctt gag tcc atc gct 595Pro Ala Lys Ser Tyr Glu Ser Leu Gly Pro Leu Leu Glu Ser Ile Ala150 155 160 165gcc aac gtt gac ggc acc cca tgt gtc acc cac atc ggc cca gac ggc 643Ala Asn Val Asp Gly Thr Pro Cys Val Thr His Ile Gly Pro Asp Gly
170 175 180gcc ggc cac ttc gtc aag atg gtc cac aac ggc atc gag tac gcc gac 691Ala Gly His Phe Val Lys Met Val His Asn Gly Ile Glu Tyr Ala Asp
185 190 195atg cag gtc atc ggc gag gca tac cac ctt ctc cgc tac gca gca ggc 739Met Gln Val Ile Gly Glu Ala Tyr His Leu Leu Arg Tyr Ala Ala Gly
200 205 210atg cag cca gct gaa atc gct gag gtt ttc aag gaa tgg aac gca ggc 787Met Gln Pro Ala Glu Ile Ala Glu Val Phe Lys Glu Trp Asn Ala Gly
215 220 225gac ctg gat tcc tac ctc atc gaa atc acc gca gag gtt ctc tcc cag 835Asp Leu Asp Ser Tyr Leu Ile Glu Ile Thr Ala Glu Val Leu Ser Gln230 235 240 245gtg gat gct gaa acc ggc aag cca cta atc gac gtc atc gtt gac gct 883Val Asp Ala Glu Thr Gly Lys Pro Leu Ile Asp Val Ile Val Asp Ala
250 255 260gca ggt cag aag ggc acc gga cgt tgg acc gtc aag gct gct ctt gat 931Ala Gly Gln Lys Gly Thr Gly Arg Trp Thr Val Lys Ala Ala Leu Asp
265 270 275ctg ggt att gct acc acc ggc atc ggc gaa gct gtt ttc gca cgt gca 979Leu Gly Ile Ala Thr Thr Gly Ile Gly Glu Ala Val Phe Ala Arg Ala
280 285 290ctc tcc ggc gca acc agc cag cgc gct gca gca cag ggc aac cta cct 1027Leu Ser Gly Ala Thr Ser Gln Arg Ala Ala Ala Gln Gly Asn Leu Pro
295 300 305gca ggt gtc ctc acc gat ctg gaa gca ctt ggc gtg gac aag gca cag 1075Ala Gly Val Leu Thr Asp Leu Glu Ala Leu Gly Val Asp Lys Ala Gln310 315 320 325ttc gtc gaa gac gtt cgc cgt gca ctg tac gca tcc aag ctt gtt gct 1123Phe Val Glu Asp Val Arg Arg Ala Leu Tyr Ala Ser Lys Leu Val Ala
330 335 340tac gca cag ggc ttc gac gag atc aag gct ggc tcc gac gag aac aac 1171Tyr Ala Gln Gly Phe Asp Glu Ile Lys Ala Gly Ser Asp Glu Asn Asn
345 350 355tgg gac gtt gac cct cgc gac ctc gct acc atc tgg cgc ggc ggc tgc 1219Trp Asp Val Asp Pro Arg Asp Leu Ala Thr Ile Trp Arg Gly Gly Cys
360 365 370atc att cgc gct aag ttc ctc aac cgc atc gtc gaa gca tac gat gca 1267Ile Ile Arg Ala Lys Phe Leu Asn Arg Ile Val Glu Ala Tyr Asp Ala
375 380 385aac gct gaa ctt gag tcc ctg ctg ctc gat cct tac ttc aag agc gag 1315Asn Ala Glu Leu Glu Ser Leu Leu Leu Asp Pro Tyr Phe Lys Ser Glu390 395 400 405ctc ggc gac ctc atc gat tca tgg cgt cgc gtg att gtc acc gcc acc 1363Leu Gly Asp Leu Ile Asp Ser Trp Arg Arg Val Ile Val Thr Ala Thr
410 415 420cag ctt ggc ctg cca atc cca gtg ttc gct tcc tcc ctg tcc tac tac 1411Gln Leu Gly Leu Pro Ile Pro Val Phe Ala Ser Ser Leu Ser Tyr Tyr
425 430 435gac agc ctg cgt gca gag cgt ctg cca gca gcc ctg atc caa gga cag 1459Asp Ser Leu Arg Ala Glu Arg Leu Pro Ala Ala Leu Ile Gln Gly Gln
440 445 450cgc gac ttc ttc ggt gcg cac acc tac aag cgc atc gac aag gat ggc 1507Arg Asp Phe Phe Gly Ala His Thr Tyr Lys Arg Ile Asp Lys Asp Gly
455 460 465tcc ttc cac acc gag tgg tcc ggc gac cgc tcc gag gtt gaa gct 1552Ser Phe His Thr Glu Trp Ser Gly Asp Arg Ser Glu Val Glu Ala470 475 480taaaggctct ccttttaaca caa 1575<210>252<211>484<212>PRT<213>谷氨酸棒杆菌<400>252Met Thr Asn Gly Asp Asn Leu Ala Gln Ile Gly Val Val Gly Leu Ala1 5 10 15Val Met Gly Ser Asn Leu Ala Arg Asn Phe Ala Arg Asn Gly Asn Thr
20 25 30Val Ala Val Tyr Asn Arg Ser Thr Asp Lys Thr Asp Lys Leu Ile Ala
35 40 45Asp His Gly Ser Glu Gly Asn Phe Ile Pro Ser Ala Thr Val Glu Glu
50 55 60Phe Val Ala Ser Leu Glu Lys Pro Arg Arg Ala Ile Ile Met Val Gln65 70 75 80Ala Gly Asn Ala Thr Asp Ala Val Ile Asn Gln Leu Ala Asp Ala Met
85 90 95Asp Glu Gly Asp Ile Ile Ile Asp Gly Gly Asn Ala Leu Tyr Thr Asp
100 105 110Thr Ile Arg Arg Glu Lys Glu Ile Ser Ala Arg Gly Leu His Phe Val
115 120 125Gly Ala Gly Ile Ser Gly Gly Glu Glu Gly Ala Leu Asn Gly Pro Ser
130 135 140Ile Met Pro Gly Gly Pro Ala Lys Ser Tyr Glu Ser Leu Gly Pro Leu145 150 155 160Leu Glu Ser Ile Ala Ala Asn Val Asp Gly Thr Pro Cys Val Thr His
165 170 175Ile Gly Pro Asp Gly Ala Gly His Phe Val Lys Met Val His Asn Gly
180 185 190Ile Glu Tyr Ala Asp Met Gln Val Ile Gly Glu Ala Tyr His Leu Leu
195 200 205Arg Tyr Ala Ala Gly Met Gln Pro Ala Glu Ile Ala Glu Val Phe Lys
210 215 220Glu Trp Asn Ala Gly Asp Leu Asp Ser Tyr Leu Ile Glu Ile Thr Ala225 230 235 240Glu Val Leu Ser Gln Val Asp Ala Glu Thr Gly Lys Pro Leu Ile Asp
245 250 255Val Ile Val Asp Ala Ala Gly Gln Lys Gly Thr Gly Arg Trp Thr Val
260 265 270Lys Ala Ala Leu Asp Leu Gly Ile Ala Thr Thr Gly Ile Gly Glu Ala
275 280 285Val Phe Ala Arg Ala Leu Ser Gly Ala Thr Ser Gln Arg Ala Ala Ala
290 295 300Gln Gly Asn Leu Pro Ala Gly Val Leu Thr Asp Leu Glu Ala Leu Gly305 310 315 320Val Asp Lys Ala Gln Phe Val Glu Asp Val Arg Arg Ala Leu Tyr Ala
325 330 335Ser Lys Leu Val Ala Tyr Ala Gln Gly Phe Asp Glu Ile Lys Ala Gly
340 345 350Ser Asp Glu Asn Asn Trp Asp Val Asp Pro Arg Asp Leu Ala Thr Ile
355 360 365Trp Arg Gly Gly Cys Ile Ile Arg Ala Lys Phe Leu Asn Arg Ile Val
370 375 380Glu Ala Tyr Asp Ala Asn Ala Glu Leu Glu Ser Leu Leu Leu Asp Pro385 390 395 400Tyr Phe Lys Ser Glu Leu Gly Asp Leu Ile Asp Ser Trp Arg Arg Val
405 410 415Ile Val Thr Ala Thr Gln Leu Gly Leu Pro Ile Pro Val Phe Ala Ser
420 425 430Ser Leu Ser Tyr Tyr Asp Ser Leu Arg Ala Glu Arg Leu Pro Ala Ala
435 440 445Leu Ile Gln Gly Gln Arg Asp Phe Phe Gly Ala His Thr Tyr Lys Arg
450 455 460Ile Asp Lys Asp Gly Ser Phe His Thr Glu Trp Ser Gly Asp Arg Ser465 470 475 480Glu Val Glu Ala<210>253<211>1537<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1537)<223>FRXA00999<400>253cctcctgtga cctggtaaaa tcgccactac ccccaaatgg tcacaccttt taggccgatt 60ttgctgacac cgggctatgc cgtcaagtac gatcaataac atg act aat gga gat 115
Met Thr Asn Gly Asp
1 5aat ctc gca cag atc ggc gtt gta ggc cta gca gta atg ggc tca aac 163Asn Leu Ala Gln Ile Gly Val Val Gly Leu Ala Val Met Gly Ser Asn
10 15 20ctc gcc cgc aac ttc gcc cgc aac ggc aac act gtc gct gtc tac aac 211Leu Ala Arg Asn Phe Ala Arg Asn Gly Asn Thr Val Ala Val Tyr Asn
25 30 35cgc agc act gac aaa acc gac aag ctc atc gcc gat cac ggc tcc gaa 259Arg Ser Thr Asp Lys Thr Asp Lys Leu Ile Ala Asp His Gly Ser Glu
40 45 50ggc aac ttc atc cct tct gca acc gtc gaa gag ttc gta gca tcc ctg 307Gly Asn Phe Ile Pro Ser Ala Thr Val Glu Glu Phe Val Ala Ser Leu
55 60 65gaa aag cca cgc cgc gcc atc atc atg gtt cag gct ggt aac gcc acc 355Glu Lys Pro Arg Arg Ala Ile Ile Met Val Gln Ala Gly Asn Ala Thr70 75 80 85gac gca gtc atc aac cag ctg gca gat gcc atg gac gaa ggc gac atc 403Asp Ala Val Ile Asn Gln Leu Ala Asp Ala Met Asp Glu Gly Asp Ile
90 95 100atc atc gac ggc ggc aac gcc ctc tac acc gac acc att cgt cgc gag 451Ile Ile Asp Gly Gly Asn Ala Leu Tyr Thr Asp Thr Ile Arg Arg Glu
105 110 115aag gaa atc tcc gca cgc ggt ctc cac ttc gtc ggt gct ggt atc tcc 499Lys Glu Ile Ser Ala Arg Gly Leu His Phe Val Gly Ala Gly Ile Ser
120 125 130ggc ggc gaa gaa ggc gca ctc aac ggc cca tcc atc atg cct ggt ggc 547Gly Gly Glu Glu Gly Ala Leu Asn Gly Pro Ser Ile Met Pro Gly Gly
135 140 145cca gca aag tcc tac gag tcc ctc gga cca ctg ctt gag tcc atc gct 595Pro Ala Lys Ser Tyr Glu Ser Leu Gly Pro Leu Leu Glu Ser Ile Ala150 155 160 165gcc aac gtt gac ggc acc cca tgt gtc acc cac atc ggc cca gac ggc 643Ala Asn Val Asp Gly Thr Pro Cys Val Thr His Ile Gly Pro Asp Gly
170 175 180gcc ggc cac ttc gtc aag atg gtc cac aac ggc atc gag tac gcc gac 691Ala Gly His Phe Val Lys Met Val His Asn Gly Ile Glu Tyr Ala Asp
185 190 195atg cag gtc atc ggc gag gca tac cac ctt ctc cgc tac gca gca ggc 739Met Gln Val Ile Gly Glu Ala Tyr His Leu Leu Arg Tyr Ala Ala Gly
200 205 210atg cag cca gct gaa atc gct gag gtt ttc aag gaa tgg aac gca ggc 787Met Gln Pro Ala Glu Ile Ala Glu Val Phe Lys Glu Trp Asn Ala Gly
215 220 225gac ctg gat tcc tac ctc atc gaa atc acc gca gag gtt ctc tcc cag 835Asp Leu Asp Ser Tyr Leu Ile Glu Ile Thr Ala Glu Val Leu Ser Gln230 235 240 245gtg gat gct gaa acc ggc aag cca cta atc gac gtc atc gtt gac gct 883Val Asp Ala Glu Thr Gly Lys Pro Leu Ile Asp Val Ile Val Asp Ala
250 255 260gca ggt cag aag ggc acc gga cgt tgg acc gtc aag gct gct ctt gat 931Ala Gly Gln Lys Gly Thr Gly Arg Trp Thr Val Lys Ala Ala Leu Asp
265 270 275ctg ggt att gct acc acc ggc atc ggc gaa gct gtt ttc gca cgt gca 979Leu Gly Ile Ala Thr Thr Gly Ile Gly Glu Ala Val Phe Ala Arg Ala
280 285 290ctc tcc ggc gca acc agc cag cgc gct gca gca cag ggc aac cta cct 1027Leu Ser Gly Ala Thr Ser Gln Arg Ala Ala Ala Gln Gly Asn Leu Pro
295 300 305gca ggt gtc ctc acc gat ctg gaa gca ctt ggc gtg gac aag gca cag 1075Ala Gly Val Leu Thr Asp Leu Glu Ala Leu Gly Val Asp Lys Ala Gln310 315 320 325ttc gtc gaa gac gtt cgc cgt gca ctg tac gca tcc aag ctt gtt gct 1123Phe Val Glu Asp Val Arg Arg Ala Leu Tyr Ala Ser Lys Leu Val Ala
330 335 340tac gca cag ggc ttc gac gag atc aag gct ggc tcc gac gag aac aac 1171Tyr Ala Gln Gly Phe Asp Glu Ile Lys Ala Gly Ser Asp Glu Asn Asn
345 350 355tgg gac gtt gac cct cgc gac ctc gct acc atc tgg cgc ggc ggc tgc 1219Trp Asp Val Asp Pro Arg Asp Leu Ala Thr Ile Trp Arg Gly Gly Cys
360 365 370atc att cgc gct aag ttc ctc aac cgc atc gtc gaa gca tac gat gca 1267Ile Ile Arg Ala Lys Phe Leu Asn Arg Ile Val Glu Ala Tyr Asp Ala
375 380 385aac gct gaa ctt gag tcc ctg ctg ctc gat cct tac ttc aag agc gag 1315Asn Ala Glu Leu Glu Ser Leu Leu Leu Asp Pro Tyr Phe Lys Ser Glu390 395 400 405ctc ggc gac ctc atc gat tca tgg cgt cgc gtg att gtc acc gcc acc 1363Leu Gly Asp Leu Ile Asp Ser Trp Arg Arg Val Ile Val Thr Ala Thr
410 415 420cag ctt ggc ctg cca atc cca gtg ttc gct tcc tcc ctg tcc tac tac 1411Gln Leu Gly Leu Pro Ile Pro Val Phe Ala Ser Ser Leu Ser Tyr Tyr
425 430 435gac agc ctg cgt gca gag cgt ctg cca gca gcc ctg atc caa gga cag 1459Asp Ser Leu Arg Ala Glu Arg Leu Pro Ala Ala Leu Ile Gln Gly Gln
440 445 450cgc gac ttc ttc ggt gcg cac acc tac aag cgc atc gac aag gat ggc 1507Arg Asp Phe Phe Gly Ala His Thr Tyr Lys Arg Ile Asp Lys Asp Gly
455 460 465tcc ttc cac acc gag tgg tcc ggc gac cgc 1537Ser Phe His Thr Glu Trp Ser Gly Asp Arg470 475<210>254<211>479<212>PRT<213>谷氨酸棒杆菌<400>254Met Thr Asn Gly Asp Asn Leu Ala Gln Ile Gly Val Val Gly Leu Ala1 5 10 15Val Met Gly Ser Asn Leu Ala Arg Asn Phe Ala Arg Asn Gly Asn Thr
20 25 30Val Ala Val Tyr Asn Arg Ser Thr Asp Lys Thr Asp Lys Leu Ile Ala
35 40 45Asp His Gly Ser Glu Gly Asn Phe Ile Pro Ser Ala Thr Val Glu Glu
50 55 60Phe Val Ala Ser Leu Glu Lys Pro Arg Arg Ala Ile Ile Met Val Gln65 70 75 80Ala Gly Asn Ala Thr Asp Ala Val Ile Asn Gln Leu Ala Asp Ala Met
85 90 95Asp Glu Gly Asp Ile Ile Ile Asp Gly Gly Asn Ala Leu Tyr Thr Asp
100 105 110Thr Ile Arg Arg Glu Lys Glu Ile Ser Ala Arg Gly Leu His Phe Val
115 120 125Gly Ala Gly Ile Ser Gly Gly Glu Glu Gly Ala Leu Asn Gly Pro Ser
130 135 140Ile Met Pro Gly Gly Pro Ala Lys Ser Tyr Glu Ser Leu Gly Pro Leu145 150 155 160Leu Glu Ser Ile Ala Ala Asn Val Asp Gly Thr Pro Cys Val Thr His
165 170 175Ile Gly Pro Asp Gly Ala Gly His Phe Val Lys Met Val His Asn Gly
180 185 190Ile Glu Tyr Ala Asp Met Gln Val Ile Gly Glu Ala Tyr His Leu Leu
195 200 205Arg Tyr Ala Ala Gly Met Gln Pro Ala Glu Ile Ala Glu Val Phe Lys
210 215 220Glu Trp Asn Ala Gly Asp Leu Asp Ser Tyr Leu Ile Glu Ile Thr Ala225 230 235 240Glu Val Leu Ser Gln Val Asp Ala Glu Thr Gly Lys Pro Leu Ile Asp
245 250 255Val Ile Val Asp Ala Ala Gly Gln Lys Gly Thr Gly Arg Trp Thr Val
260 265 270Lys Ala Ala Leu Asp Leu Gly Ile Ala Thr Thr Gly Ile Gly Glu Ala
275 280 285Val Phe Ala Arg Ala Leu Ser Gly Ala Thr Ser Gln Arg Ala Ala Ala
290 295 300Gln Gly Asn Leu Pro Ala Gly Val Leu Thr Asp Leu Glu Ala Leu Gly305 310 315 320Val Asp Lys Ala Gln Phe Val Glu Asp Val Arg Arg Ala Leu Tyr Ala
325 330 335Ser Lys Leu Val Ala Tyr Ala Gln Gly Phe Asp Glu Ile Lys Ala Gly
340 345 350Ser Asp Glu Asn Asn Trp Asp Val Asp Pro Arg Asp Leu Ala Thr Ile
355 360 365Trp Arg Gly Gly Cys Ile Ile Arg Ala Lys Phe Leu Asn Arg Ile Val
370 375 380Glu Ala Tyr Asp Ala Asn Ala Glu Leu Glu Ser Leu Leu Leu Asp Pro385 390 395 400Tyr Phe Lys Ser Glu Leu Gly Asp Leu Ile Asp Ser Trp Arg Arg Val
405 410 415Ile Val Thr Ala Thr Gln Leu Gly Leu Pro Ile Pro Val Phe Ala Ser
420 425 430Ser Leu Ser Tyr Tyr Asp Ser Leu Arg Ala Glu Arg Leu Pro Ala Ala
435 440 445Leu Ile Gln Gly Gln Arg Asp Phe Phe Gly Ala His Thr Tyr Lys Arg
450 455 460Ile Asp Lys Asp Gly Ser Phe His Thr Glu Trp Ser Gly Asp Arg465 470 475<210>255<211>1326<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1303)<223>RXN02596<400>255aaggtatctg ggtgtggata tgccctgcta actggagaaa cttggcccga tcgggtgtct 60gaaatttcgg caacgccgaa tgtaagttag tgtcgaatgc atg acg gaa tcg aaa 115
Met Thr Glu Ser Lys
1 5aat tac gac tta atc gtt gta ggc tcc ggc ctc ttc ggg ctc acc gtg 163Asn Tyr Asp Leu Ile Val Val Gly Ser Gly Leu Phe Gly Leu Thr Val
10 15 20gct gag cgt gca gct agc cag ctg ggt aag aaa gtc ctc atc gtt gaa 211Ala Glu Arg Ala Ala Ser Gln Leu Gly Lys Lys Val Leu Ile Val Glu
25 30 35cgc cgc tcg cac ctc ggt ggc aat gct tac tct gaa gca gaa cca gag 259Arg Arg Ser His Leu Gly Gly Asn Ala Tyr Ser Glu Ala Glu Pro Glu
40 45 50acc ggc att gaa atc cac aaa tac ggc gcg cac ctc ttc cac acc tcc 307Thr Gly Ile Glu Ile His Lys Tyr Gly Ala His Leu Phe His Thr Ser
55 60 65aac aca cgc gtg tgg gaa tac gtc aac cag ttc acc agt ttc acc ggc 355Asn Thr Arg Val Trp Glu Tyr Val Asn Gln Phe Thr Ser Phe Thr Gly70 75 80 85tac cag cac cgc gtc ttc gca atg cac aac ggc acc gcc tac caa ttc 403Tyr Gln His Arg Val Phe Ala Met His Asn Gly Thr Ala Tyr Gln Phe
90 95 100ccc atg gga ctg ggc ctg att aac cag ttc ttc ggc aag tac tac agc 451Pro Met Gly Leu Gly Leu Ile Asn Gln Phe Phe Gly Lys Tyr Tyr Ser
105 110 115cca gat gaa gcc cgt gag ctc atc aag gaa cag tct gca gaa atc gat 499Pro Asp Glu Ala Arg Glu Leu Ile Lys Glu Gln Ser Ala Glu Ile Asp
120 125 130tcc tcc gac gcc acc aac ctc gaa gaa aag gcc att tcc ctc att ggt 547Ser Ser Asp Ala Thr Asn Leu Glu Glu Lys Ala Ile Ser Leu Ile Gly
135 140 145cgc cca ctt tac gag gca ttc atc cgc gac tac acc gca aag cag tgg 595Arg Pro Leu Tyr Glu Ala Phe Ile Arg Asp Tyr Thr Ala Lys Gln Trp150 155 160 165cag act gat cca aag aac ctc cca gcc ggc aac atc acc cgc ctg cca 643Gln Thr Asp Pro Lys Asn Leu Pro Ala Gly Asn Ile Thr Arg Leu Pro
170 175 180gtt cgc tac aac ttc aac aac cgc tat ttc aac gac acc tac gaa ggc 691Val Arg Tyr Asn Phe Asn Asn Arg Tyr Phe Asn Asp Thr Tyr Glu Gly
185 190 195ctt ccc aca gac ggc tac gcg gca tgg ttg gaa aag atg gca gag cat 739Leu Pro Thr Asp Gly Tyr Ala Ala Trp Leu Glu Lys Met Ala Glu His
200 205 210gag ctt atc gac gtc cgc ctc gac acc gac tgg ttc gac gtt cgc gat 787Glu Leu Ile Asp Val Arg Leu Asp Thr Asp Trp Phe Asp Val Arg Asp
215 220 225gac ctc cgc gca agc aac ccc gac gca cct gtg gtc tac acc ggc cca 835Asp Leu Arg Ala Ser Asn Pro Asp Ala Pro Val Val Tyr Thr Gly Pro230 235 240 245ctc gac ctc tac ttc aac tac gca gag ggc aag ctg gga tgg cgc acc 883Leu Asp Leu Tyr Phe Asn Tyr Ala Glu Gly Lys Leu Gly Trp Arg Thr
250 255 260ctc gac ttt gaa acc gaa gta gta gaa acc ggt gac ttc caa gga acc 931Leu Asp Phe Glu Thr Glu Val Val Glu Thr Gly Asp Phe Gln Gly Thr
265 270 275cca gtg atg aac tac aac gat gcg gac gta cct ttc acc cgc atc cac 979Pro Val Met Asn Tyr Asn Asp Ala Asp Val Pro Phe Thr Arg Ile His
280 285 290gag ttc cgt cac ttc cac cca gag cgt gat gac agt tac ccc aag gat 1027Glu Phe Arg His Phe His Pro Glu Arg Asp Asp Ser Tyr Pro Lys Asp
295 300 305aag acc gtc atc atg cgc gag ttc tcc cgt ttc gca gat aac gag gat 1075Lys Thr Val Ile Met Arg Glu Phe Ser Arg Phe Ala Asp Asn Glu Asp310 315 320 325gag cct tat tac cca atc aac act cca gac gac cga gac atg ctg aag 1123Glu Pro Tyr Tyr Pro Ile Asn Thr Pro Asp Asp Arg Asp Met Leu Lys
330 335 340cag tac cgc ctt ctg gct gct gaa gag gct gct aat aat aag gtg ctg 1171Gln Tyr Arg Leu Leu Ala Ala Glu Glu Ala Ala Asn Asn Lys Val Leu
345 350 355ttc ggc ggt cga ctg ggc acg tac cag tac ctc gac atg cac atg gct 1219Phe Gly Gly Arg Leu Gly Thr Tyr Gln Tyr Leu Asp Met His Met Ala
360 365 370atc ggt tct gcg ctg agc atg ttt gac aac aag ctg gtg ccg ttc ttt 1267Ile Gly Ser Ala Leu Ser Met Phe Asp Asn Lys Leu Val Pro Phe Phe
375 380 385gaa gaa ggc aca ccg cta gag cag gaa cgc gga cac taaaaggaag 1313Glu Glu Gly Thr Pro Leu Glu Gln Glu Arg Gly His390 395 400ggcatctccc aca 1326<210>256<211>401<212>PRT<213>谷氨酸棒杆菌<400>256Met Thr Glu Ser Lys Asn Tyr Asp Leu Ile Val Val Gly Ser Gly Leu1 5 10 15Phe Gly Leu Thr Val Ala Glu Arg Ala Ala Ser Gln Leu Gly Lys Lys
20 25 30Val Leu Ile Val Glu Arg Arg Ser His Leu Gly Gly Asn Ala Tyr Ser
35 40 45Glu Ala Glu Pro Glu Thr Gly Ile Glu Ile His Lys Tyr Gly Ala His
50 55 60Leu Phe His Thr Ser Asn Thr Arg Val Trp Glu Tyr Val Asn Gln Phe65 70 75 80Thr Ser Phe Thr Gly Tyr Gln His Arg Val Phe Ala Met His Asn Gly
85 90 95Thr Ala Tyr Gln Phe Pro Met Gly Leu Gly Leu Ile Asn Gln Phe Phe
100 105 110Gly Lys Tyr Tyr Ser Pro Asp Glu Ala Arg Glu Leu Ile Lys Glu Gln
115 120 125Ser Ala Glu Ile Asp Ser Ser Asp Ala Thr Asn Leu Glu Glu Lys Ala
130 135 140Ile Ser Leu Ile Gly Arg Pro Leu Tyr Glu Ala Phe Ile Arg Asp Tyr145 150 155 160Thr Ala Lys Gln Trp Gln Thr Asp Pro Lys Asn Leu Pro Ala Gly Asn
165 170 175Ile Thr Arg Leu Pro Val Arg Tyr Asn Phe Asn Asn Arg Tyr Phe Asn
180 185 190Asp Thr Tyr Glu Gly Leu Pro Thr Asp Gly Tyr Ala Ala Trp Leu Glu
195 200 205Lys Met Ala Glu His Glu Leu Ile Asp Val Arg Leu Asp Thr Asp Trp
210 215 220Phe Asp Val Arg Asp Asp Leu Arg Ala Ser Asn Pro Asp Ala Pro Val225 230 235 240Val Tyr Thr Gly Pro Leu Asp Leu Tyr Phe Asn Tyr Ala Glu Gly Lys
245 250 255Leu Gly Trp Arg Thr Leu Asp Phe Glu Thr Glu Val Val Glu Thr Gly
260 265 270Asp Phe Gln Gly Thr Pro Val Met Asn Tyr Asn Asp Ala Asp Val Pro
275 280 285Phe Thr Arg Ile His Glu Phe Arg His Phe His Pro Glu Arg Asp Asp
290 295 300Ser Tyr Pro Lys Asp Lys Thr Val Ile Met Arg Glu Phe Ser Arg Phe305 310 315 320Ala Asp Asn Glu Asp Glu Pro Tyr Tyr Pro Ile Asn Thr Pro Asp Asp
325 330 335Arg Asp Met Leu Lys Gln Tyr Arg Leu Leu Ala Ala Glu Glu Ala Ala
340 345 350Asn Asn Lys Val Leu Phe Gly Gly Arg Leu Gly Thr Tyr Gln Tyr Leu
355 360 365Asp Met His Met Ala Ile Gly Ser Ala Leu Ser Met Phe Asp Asn Lys
370 375 380Leu Val Pro Phe Phe Glu Glu Gly Thr Pro Leu Glu Gln Glu Arg Gly385 390 395 400His<210>257<211>512<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(489)<223>FRXA02596<400>257cct gtg gtc tac acc ggc cca ctc gac ctc tac ttc aac tac gca gag 48Pro Val Val Tyr Thr Gly Pro Leu Asp Leu Tyr Phe Asn Tyr Ala Glu1 5 10 15ggc aag ctg gga tgg cgc acc ctc gac ttt gaa acc gaa gta gta gaa 96Gly Lys Leu Gly Trp Arg Thr Leu Asp Phe Glu Thr Glu Val Val Glu
20 25 30acc ggt gac ttc caa gga acc cca gtg atg aac tac aac gat gcg gac 144Thr Gly Asp Phe Gln Gly Thr Pro Val Met Asn Tyr Asn Asp Ala Asp
35 40 45gta cct ttc acc cgc atc cac gag ttc cgt cac ttc cac cca gag cgt 192Val Pro Phe Thr Arg Ile His Glu Phe Arg His Phe His Pro Glu Arg
50 55 60gat gac agt tac ccc aag gat aag acc gtc atc atg cgc gag ttc tcc 240Asp Asp Ser Tyr Pro Lys Asp Lys Thr Val Ile Met Arg Glu Phe Ser65 70 75 80cgt ttc gca gat aac gag gat gag cct tat tac cca atc aac act cca 288Arg Phe Ala Asp Asn Glu Asp Glu Pro Tyr Tyr Pro Ile Asn Thr Pro
85 90 95gac gac cga gac atg ctg aag cag tac cgc ctt ctg gct gct gaa gag 336Asp Asp Arg Asp Met Leu Lys Gln Tyr Arg Leu Leu Ala Ala Glu Glu
100 105 110gct gct aat aat aag gtg ctg ttc ggc ggt cga ctg ggc acg tac cag 384Ala Ala Asn Asn Lys Val Leu Phe Gly Gly Arg Leu Gly Thr Tyr Gln
115 120 125tac ctc gac atg cac atg gct atc ggt tct gcg ctg agc atg ttt gac 432Tyr Leu Asp Met His Met Ala Ile Gly Ser Ala Leu Ser Met Phe Asp
130 135 140aac aag ctg gtg ccg ttc ttt gaa gaa ggc aca ccg cta gag cag gaa 480Asn Lys Leu Val Pro Phe Phe Glu Glu Gly Thr Pro Leu Glu Gln Glu145 150 155 160cgc gga cac taaaaggaag ggcatctccc aca 512Arg Gly His<210>258<211>163<212>PRT<213>谷氨酸棒杆菌<400>258Pro Val Val Tyr Thr Gly Pro Leu Asp Leu Tyr Phe Asn Tyr Ala Glu1 5 10 15Gly Lys Leu Gly Trp Arg Thr Leu Asp Phe Glu Thr Glu Val Val Glu
20 25 30Thr Gly Asp Phe Gln Gly Thr Pro Val Met Asn Tyr Asn Asp Ala Asp
35 40 45Val Pro Phe Thr Arg Ile His Glu Phe Arg His Phe His Pro Glu Arg
50 55 60Asp Asp Ser Tyr Pro Lys Asp Lys Thr Val Ile Met Arg Glu Phe Ser65 70 75 80Arg Phe Ala Asp Asn Glu Asp Glu Pro Tyr Tyr Pro Ile Asn Thr Pro
85 90 95Asp Asp Arg Asp Met Leu Lys Gln Tyr Arg Leu Leu Ala Ala Glu Glu
100 105 110Ala Ala Asn Asn Lys Val Leu Phe Gly Gly Arg Leu Gly Thr Tyr Gln
115 120 125Tyr Leu Asp Met His Met Ala Ile Gly Ser Ala Leu Ser Met Phe Asp
130 135 140Asn Lys Leu Val Pro Phe Phe Glu Glu Gly Thr Pro Leu Glu Gln Glu145 150 155 160Arg Gly His<210>259<211>598<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(598)<223>FRXA02642<400>259aaggtatctg ggtgtggata tgccctgcta actggagaaa cttggcccga tcgggtgtct 60gaaatttcgg caacgccgaa tgtaagttag tgtcgaatgc atg acg gaa tcg aaa 115
Met Thr Glu Ser Lys
1 5aat tac gac tta atc gtt gta ggc tcc ggc ctc ttc ggg ctc acc gtg 163Asn Tyr Asp Leu Ile Val Val Gly Ser Gly Leu Phe Gly Leu Thr Val
10 15 20gct gag cgt gca gct agc cag ctg ggt aag aaa gtc ctc atc gtt gaa 211Ala Glu Arg Ala Ala Ser Gln Leu Gly Lys Lys Val Leu Ile Val Glu
25 30 35cgc cgc tcg cac ctc ggt ggc aat gct tac tct gaa gca gaa cca gag 259Arg Arg Ser His Leu Gly Gly Asn Ala Tyr Ser Glu Ala Glu Pro Glu
40 45 50acc ggc att gaa atc cac aaa tac ggc gcg cac ctc ttc cac acc tcc 307Thr Gly Ile Glu Ile His Lys Tyr Gly Ala His Leu Phe His Thr Ser
55 60 65aac aca cgc gtg tgg gaa tac gtc aac cag ttc acc agt ttc acc ggc 355Asn Thr Arg Val Trp Glu Tyr Val Asn Gln Phe Thr Ser Phe Thr Gly70 75 80 85tac cag cac cgc gtc ttc gca atg cac aac ggc acc gcc tac caa ttc 403Tyr Gln His Arg Val Phe Ala Met His Asn Gly Thr Ala Tyr Gln Phe
90 95 100ccc atg gga ctg ggc ctg att aac cag ttc ttc ggc aag tac tac agc 451Pro Met Gly Leu Gly Leu Ile Asn Gln Phe Phe Gly Lys Tyr Tyr Ser
105 110 115cca gat gaa gcc cgt gag ctc atc aag gaa cag tct gca gaa atc gat 499Pro Asp Glu Ala Arg Glu Leu Ile Lys Glu Gln Ser Ala Glu Ile Asp
120 125 130tcc tcc gac gcc acc aac ctc gaa gaa aag gcc att tcc ctc att ggt 547Ser Ser Asp Ala Thr Asn Leu Glu Glu Lys Ala Ile Ser Leu Ile Gly
135 140 145cgc cca ctt tac gag gca ttc atc cgc gac tac acc gea aag cag tgg 595Arg Pro Leu Tyr Glu Ala Phe Ile Arg Asp Tyr Thr Ala Lys Gln Trp150 155 160 165cag 598Gln<210>260<21l>166<212>PRT<213>谷氨酸棒杆菌<400>260Met Thr Glu Ser Lys Asn Tyr Asp Leu Ile Val Val Gly Ser Gly Leu1 5 10 15Phe Gly Leu Thr Val Ala Glu Arg Ala Ala Ser Gln Leu Gly Lys Lys
20 25 30Val Leu Ile Val Glu Arg Arg Ser His Leu Gly Gly Asn Ala Tyr Ser
35 40 45Glu Ala Glu Pro Glu Thr Gly Ile Glu Ile His Lys Tyr Gly Ala His
50 55 60Leu Phe His Thr Ser Asn Thr Arg Val Trp Glu Tyr Val Asn Gln Phe65 70 75 80Thr Ser Phe Thr Gly Tyr Gln His Arg Val Phe Ala Met His Asn Gly
85 90 95Thr Ala Tyr Gln Phe Pro Met Gly Leu Gly Leu Ile Asn Gln Phe Phe
100 105 110Gly Lys Tyr Tyr Ser Pro Asp Glu Ala Arg Glu Leu Ile Lys Glu Gln
115 120 125Ser Ala Glu Ile Asp Ser Ser Asp Ala Thr Asn Leu Glu Glu Lys Ala
130 135 140Ile Ser Leu Ile Gly Arg Pro Leu Tyr Glu Ala Phe Ile Arg Asp Tyr145 150 155 160Thr Ala Lys Gln Trp Gln
165<210>261<211>668<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(645)<223>RXA02572<400>261gcg gtc gct gag att tgc gag ccg acc ggc gcc gat gcg gtt gcg ctt 48Ala Val Ala Glu Ile Cys Glu Pro Thr Gly Ala Asp Ala Val Ala Leu1 5 10 15gtg gat gcc atc ggt cac gac gat cgt atc ggc cga aag ttc tta ggc 96Val Asp Ala Ile Gly His Asp Asp Arg Ile Gly Arg Lys Phe Leu Gly
20 25 30gcg ggc ctg gga ttc ggt ggc ggt tgt ttg cct aaa gac atc cgc gct 144Ala Gly Leu Gly Phe Gly Gly Gly Cys Leu Pro Lys Asp Ile Arg Ala
35 40 45ttc atg gca cgc gcg ggc gaa ttg ggt gct gac cag gca tta acg ttc 192Phe Met Ala Arg Ala Gly Glu Leu Gly Ala Asp Gln Ala Leu Thr Phe
50 55 60ttg cgt gag gtc gat tcc atc aat atg cgt cgt cgc gac cgt gtg gtg 240Leu Arg Glu Val Asp Ser Ile Asn Met Arg Arg Arg Asp Arg Val Val65 70 75 80cag ctg gcc aaa gag atg tgt ggc ggt tcg ctg ctg ggc aag cgg gtt 288Gln Leu Ala Lys Glu Met Cys Gly Gly Ser Leu Leu Gly Lys Arg Val
85 90 95aca gtg ctc ggc gcc gca ttc aaa ccc aac tcg gac gat gtc cgc gat 336Thr Val Leu Gly Ala Ala Phe Lys Pro Asn Ser Asp Asp Val Arg Asp
100 105 110tct ccg gcg ctg tcg gtc gcg ggt tcg ctg tcg ctc cag ggt gcg gcg 384Ser Pro Ala Leu Ser Val Ala Gly Ser Leu Ser Leu Gln Gly Ala Ala
115 120 125gtc tcg gtc tac gac ccg gaa gct atg gac aac gct cga cgc gtc ttc 432Val Ser Val Tyr Asp Pro Glu Ala Met Asp Asn Ala Arg Arg Val Phe
130 135 140ccg acg ctc agc tat gcg tcc agc act aaa gag gcg ctt atc gac gcc 480Pro Thr Leu Ser Tyr Ala Ser Ser Thr Lys Glu Ala Leu Ile Asp Ala145 150 155 160cac ctc gtc gtt ctt gcc act gaa tgg caa gaa ttc cgc gac ctt gac 528His Leu Val Val Leu Ala Thr Glu Trp Gln Glu Phe Arg Asp Leu Asp
165 170 175ccc gaa gtg gcg gga ggg gtc gtc gag aag cgc gct att att gat ggc 576Pro Glu Val Ala Gly Gly Val Val Glu Lys Arg Ala Ile Ile Asp Gly
180 185 190cga aac gtc ctc gat gtt gcc aaa tgg aag gcc gcc ggt tgg gaa atg 624Arg Asn Val Leu Asp Val Ala Lys Trp Lys Ala Ala Gly Trp Glu Met
195 200 205gaa gcg ctc ggc cgc aac ctt tagtgcggtg gatcaggcgg ggc 668Glu Ala Leu Gly Arg Asn Leu
210 215<210>262<211>215<212>PRT<213>谷氨酸棒杆菌<400>262Ala Val Ala Glu Ile Cys Glu Pro Thr Gly Ala Asp Ala Val Ala Leu1 5 10 15Val Asp Ala Ile Gly His Asp Asp Arg Ile Gly Arg Lys Phe Leu Gly
20 25 30Ala Gly Leu Gly Phe Gly Gly Gly Cys Leu Pro Lys Asp Ile Arg Ala
35 40 45Phe Met Ala Arg Ala Gly Glu Leu Gly Ala Asp Gln Ala Leu Thr Phe
50 55 60Leu Arg Glu Val Asp Ser Ile Asn Met Arg Arg Arg Asp Arg Val Val65 70 75 80Gln Leu Ala Lys Glu Met Cys Gly Gly Ser Leu Leu Gly Lys Arg Val
85 90 95Thr Val Leu Gly Ala Ala Phe Lys Pro Asn Ser Asp Asp Val Arg Asp
100 105 110Ser Pro Ala Leu Ser Val Ala Gly Ser Leu Ser Leu Gln Gly Ala Ala
115 120 125Val Ser Val Tyr Asp Pro Glu Ala Met Asp Asn Ala Arg Arg Val Phe
130 135 140Pro Thr Leu Ser Tyr Ala Ser Ser Thr Lys Glu Ala Leu Ile Asp Ala145 150 155 160His Leu Val Val Leu Ala Thr Glu Trp Gln Glu Phe Arg Asp Leu Asp
165 170 175Pro Glu Val Ala Gly Gly Val Val Glu Lys Arg Ala Ile Ile Asp Gly
180 185 190Arg Asn Val Leu Asp Val Ala Lys Trp Lys Ala Ala Gly Trp Glu Met
195 200 205Glu Ala Leu Gly Arg Asn Leu
210 215<210>263<211>1224<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1201)<223>RXA02485<400>263cggtggtcag tgcttggtgc accttgccga cgggctgatt gatcgtaatg gtgttttctg 60tacgcgttgc catgaggata agactaccgt tagtggggtg ttg gat tca tcg cta 115
Leu Asp Ser Ser Leu
1 5gcc cag gaa atc gcc gcg atc gac ggc gtc gaa ctc gat tcg gaa gtc 163Ala Gln Glu Ile Ala Ala Ile Asp Gly Val Glu Leu Asp Ser Glu Val
10 15 20act ttc gcc gat ctg acg acc ctc cgc atc ggc gga aaa ccc cgc agc 211Thr Phe Ala Asp Leu Thr Thr Leu Arg Ile Gly Gly Lys Pro Arg Ser
25 30 35gcc gta cgt tgc cag acc acg gag gcg ctg gtc agc gcc ata aaa ttg 259Ala Val Arg Cys Gln Thr Thr Glu Ala Leu Val Ser Ala Ile Lys Leu
40 45 50ctt gac gac gcc tcc ctc ccc ctc ctc att gtc ggc ggc ggg tcc aat 307Leu Asp Asp Ala Ser Leu Pro Leu Leu Ile Val Gly Gly Gly Ser Asn
55 60 65ctc gtc gtg gcc gac ggc gat ctg gat gtt att gcc gtc atc atc gaa 355Leu Val Val Ala Asp Gly Asp Leu Asp Val Ile Ala Val Ile Ile Glu70 75 80 85acc gac gac gtc tcc atc aac ctc acc gac ggt ctc ctc acc gcc gat 403Thr Asp Asp Val Ser Ile Asn Leu Thr Asp Gly Leu Leu Thr Ala Asp
90 95 100gca ggc gct gtt tgg gac gat gtt gtc cac ctt tcg gtg gat gcc ggc 451Ala Gly Ala Val Trp Asp Asp Val Val His Leu Ser Val Asp Ala Gly
105 110 115ctc ggt gga att gaa tgc ctc tcc gga atc ccc ggc tcc gcc ggc gcc 499Leu Gly Gly Ile Glu Cys Leu Ser Gly Ile Pro Gly Ser Ala Gly Ala
120 125 130acc cca gtc caa aac gtg ggc gcc tac ggc acg gaa gtt tcc gat gta 547Thr Pro Val Gln Asn Val Gly Ala Tyr Gly Thr Glu Val Ser Asp Val
135 140 145ctc acc cgc gtc cag ctt ctc gac cgc acc acc cac caa gtc tcc tgg 595Leu Thr Arg Val Gln Leu Leu Asp Arg Thr Thr His Gln Val Ser Trp150 155 160 165gtc gac gcc tcc gaa ctc gac ctc tct tac cga tac tcc aat ctc aaa 643Val Asp Ala Ser Glu Leu Asp Leu Ser Tyr Arg Tyr Ser Asn Leu Lys
170 175 180ttc acc aac cgc gca gtc gtc ttg gcg atc gaa ctc cag ctc ctc acc 691Phe Thr Asn Arg Ala Val Val Leu Ala Ile Glu Leu Gln Leu Leu Thr
185 190 195gac gga ttg tcc gcg ccg cta cgt ttt ggt gaa ttg gga cgt cga tta 739Asp Gly Leu Ser Ala Pro Leu Arg Phe Gly Glu Leu Gly Arg Arg Leu
200 205 210gcg atc tcc gag gcc gaa ccc cac cca cgt cgc ccc gtc cgc atg gtc 787Ala Ile Ser Glu Ala Glu Pro His Pro Arg Arg Pro Val Arg Met Val
215 220 225cgc gac gcc gtc cta gaa ctc cgc cgc gcc aaa ggc atg gtc gtg gaa 835Arg Asp Ala Val Leu Glu Leu Arg Arg Ala Lys Gly Met Val Val Glu230 235 240 245cac acc gac cac gac acc tgg tcc gcc gga tcc ttc ttc acc aac cca 883His Thr Asp His Asp Thr Trp Ser Ala Gly Ser Phe Phe Thr Asn Pro
250 255 260atc gtc gac cca gcc ctt gcc gac gca gtc ttt gaa aaa gtc ggc gaa 931Ile Val Asp Pro Ala Leu Ala Asp Ala Val Phe Glu Lys Val Gly Glu
265 270 275ccc acc atg ccc cgc ttc cca gcc ggc gat ggc aaa gaa aaa ctc tcc 979Pro Thr Met Pro Arg Phe Pro Ala Gly Asp Gly Lys Glu Lys Leu Ser
280 285 290gca gcc tgg ctc atc gaa cgc gcc ggc ttc aaa aag gga cac ccc ggc 1027Ala Ala Trp Leu Ile Glu Arg Ala Gly Phe Lys Lys Gly His Pro Gly
295 300 305gca ggc gca aaa gcc tcc ctg agc acc aaa cac acc ctc gca ctc acc 1075Ala Gly Ala Lys Ala Ser Leu Ser Thr Lys His Thr Leu Ala Leu Thr310 315 320 325aac cgt ggc gac gcc cgc gcc tcc gac ctc gtc gca tta gcc aaa gaa 1123Asn Arg Gly Asp Ala Arg Ala Ser Asp Leu Val Ala Leu Ala Lys Glu
330 335 340atc cgc gac gga gtc ctc gaa acc ttc ggc gtc acc ctc gtc cca gaa 1171Ile Arg Asp Gly Val Leu Glu Thr Phe Gly Val Thr Leu Val Pro Glu
345 350 355ccc gtc tgg att gga atc agc atc gat gac tgaattttcc gacgtccctg 1221Pro Val Trp Ile Gly Ile Ser Ile Asp Asp
360 365gca 1224<210>264<211>367<212>PRT<213>谷氨酸棒杆菌<400>264Leu Asp Ser Ser Leu Ala Gln Glu Ile Ala Ala Ile Asp Gly Val Glu1 5 10 15Leu Asp Ser Glu Val Thr Phe Ala Asp Leu Thr Thr Leu Arg Ile Gly
20 25 30Gly Lys Pro Arg Ser Ala Val Arg Cys Gln Thr Thr Glu Ala Leu Val
35 40 45Ser Ala Ile Lys Leu Leu Asp Asp Ala Ser Leu Pro Leu Leu Ile Val
50 55 60Gly Gly Gly Ser Asn Leu Val Val Ala Asp Gly Asp Leu Asp Val Ile65 70 75 80Ala Val Ile Ile Glu Thr Asp Asp Val Ser Ile Asn Leu Thr Asp Gly
85 90 95Leu Leu Thr Ala Asp Ala Gly Ala Val Trp Asp Asp Val Val His Leu
100 105 110Ser Val Asp Ala Gly Leu Gly Gly Ile Glu Cys Leu Ser Gly Ile Pro
115 120 125Gly Ser Ala Gly Ala Thr Pro Val Gln Asn Val Gly Ala Tyr Gly Thr
130 135 140Glu Val Ser Asp Val Leu Thr Arg Val Gln Leu Leu Asp Arg Thr Thr145 150 155 160His Gln Val Ser Trp Val Asp Ala Ser Glu Leu Asp Leu Ser Tyr Arg
165 170 175Tyr Ser Asn Leu Lys Phe Thr Asn Arg Ala Val Val Leu Ala Ile Glu
180 185 190Leu Gln Leu Leu Thr Asp Gly Leu Ser Ala Pro Leu Arg Phe Gly Glu
195 200 205Leu Gly Arg Arg Leu Ala Ile Ser Glu Ala Glu Pro His Pro Arg Arg
210 215 220Pro Val Arg Met Val Arg Asp Ala Val Leu Glu Leu Arg Arg Ala Lys225 230 235 240Gly Met Val Val Glu His Thr Asp His Asp Thr Trp Ser Ala Gly Ser
245 250 255Phe Phe Thr Asn Pro Ile Val Asp Pro Ala Leu Ala Asp Ala Val Phe
260 265 270Glu Lys Val Gly Glu Pro Thr Met Pro Arg Phe Pro Ala Gly Asp Gly
275 280 285Lys Glu Lys Leu Ser Ala Ala Trp Leu Ile Glu Arg Ala Gly Phe Lys
290 295 300Lys Gly His Pro Gly Ala Gly Ala Lys Ala Ser Leu Ser Thr Lys His305 310 315 320Thr Leu Ala Leu Thr Asn Arg Gly Asp Ala Arg Ala Ser Asp Leu Val
325 330 335Ala Leu Ala Lys Glu Ile Arg Asp Gly Val Leu Glu Thr Phe Gly Val
340 345 350Thr Leu Val Pro Glu Pro Val Trp Ile Gly Ile Ser Ile Asp Asp
355 360 365<210>265<211>1124<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1101)<223>RXA01216<400>265acc gac cac act ctg tct gca ctg ctg gat gca cac gtg gaa gtt cca 48Thr Asp His Thr Leu Ser Ala Leu Leu Asp Ala His Val Glu Val Pro1 5 10 15acc gct gtc acc gtg ttg acc atg cgt ctg gat gac ccc acc ggc tac 96Thr Ala Val Thr Val Leu Thr Met Arg Leu Asp Asp Pro Thr Gly Tyr
20 25 30ggc cgc atc gtg cgc aac gaa gaa ggc gaa gtc acc gcc atc gtt gag 144Gly Arg Ile Val Arg Asn Glu Glu Gly Glu Val Thr Ala Ile Val Glu
35 40 45caa aaa gat gct tca gca gaa gtc caa gcc atc gat gag gtc aac tcc 192Gln Lys Asp Ala Ser Ala Glu Val Gln Ala Ile Asp Glu Val Asn Ser
50 55 60ggt gtc ttt gct ttc gac gcc gcc atc ttg cgt tcc gca ctg gct gaa 240Gly Val Phe Ala Phe Asp Ala Ala Ile Leu Arg Ser Ala Leu Ala Glu65 70 75 80ctg aag tcc gac aac gct cag ggc gag ctg tac ctg acc gac gtt ttg 288Leu Lys Ser Asp Asn Ala Gln Gly Glu Leu Tyr Leu Thr Asp Val Leu
85 90 95ggc att gct cgt ggc gag ggc cac cca gtg cgc gcc cac acc gcc gcc 336Gly Ile Ala Arg Gly Glu Gly His Pro Val Arg Ala His Thr Ala Ala
100 105 110gat gct cgt gaa ctc gcc ggc gtc aac gat cgt gtg cag ctc gca gaa 384Asp Ala Arg Glu Leu Ala Gly Val Asn Asp Arg Val Gln Leu Ala Glu
115 120 125gcc ggc gcc gaa cta aac cgt cgc acc gtc atc gcc gct atg cgt ggt 432Ala Gly Ala Glu Leu Asn Arg Arg Thr Val Ile Ala Ala Met Arg Gly
130 135 140ggc gca acc atc gtt gat cca gca acc acc tgg atc gat gtg gag gtt 480Gly Ala Thr Ile Val Asp Pro Ala Thr Thr Trp Ile Asp Val Glu Val145 150 155 160tct atc gga cgc gac gtg atc atc cac cct ggc acc cag ctc aag ggc 528Ser Ile Gly Arg Asp Val Ile Ile His Pro Gly Thr Gln Leu Lys Gly
165 170 175gaa act gtc atc gga gac cgc gtt gaa gtt ggt cca gac acc acc ttg 576Glu Thr Val Ile Gly Asp Arg Val Glu Val Gly Pro Asp Thr Thr Leu
180 185 190acc aac atg acc atc ggc gac ggc gca tcc gta atc cgc acc cac ggt 624Thr Asn Met Thr Ile Gly Asp Gly Ala Ser Val Ile Arg Thr His Gly
195 200 205ttc gac tcc acc atc ggt gaa aac gcc acc gtt ggc ccc ttc acc tac 672Phe Asp Ser Thr Ile Gly Glu Asn Ala Thr Val Gly Pro Phe Thr Tyr
210 215 220atc cgc cca gga acc aca ctg gga cca gaa ggc aag ctc ggt ggc ttc 720Ile Arg Pro Gly Thr Thr Leu Gly Pro Glu Gly Lys Leu Gly Gly Phe225 230 235 240gta gaa acc aag aag gcc aca atc ggc cgt ggc tcc aag gtt cca cac 768Val Glu Thr Lys Lys Ala Thr Ile Gly Arg Gly Ser Lys Val Pro His
245 250 255ctc acc tat gtc ggc gac gcc acc atc ggc gag gaa tcc aac atc gga 816Leu Thr Tyr Val Gly Asp Ala Thr Ile Gly Glu Glu Ser Asn Ile Gly
260 265 270gcc tcc tct gtc ttc gtg aac tac gac ggt gaa aac aag cac cac acc 864Ala Ser Ser Val Phe Val Asn Tyr Asp Gly Glu Asn Lys His His Thr
275 280 285acc atc ggc agc cac gtt cgc act ggt tct gac acc atg ttt atc gct 912Thr Ile Gly Ser His Val Arg Thr Gly Ser Asp Thr Met Phe Ile Ala
290 295 300cca gtg acc gtg ggt gac gga gcg tat tcc gga gcc ggt aca gta att 960Pro Val Thr Val Gly Asp Gly Ala Tyr Ser Gly Ala Gly Thr Val Ile305 310 315 320aaa gac gat gtt ccg cca gga gcc ctt gcc gtg tcc ggc gga cgc caa 1008Lys Asp Asp Val Pro Pro Gly Ala Leu Ala Val Ser Gly Gly Arg Gln
325 330 335cga aac atc gaa ggc tgg gtg caa aag aag cgc cct gga acc gct gca 1056Arg Asn Ile Glu Gly Trp Val Gln Lys Lys Arg Pro Gly Thr Ala Ala
340 345 350gca caa gcc gca gaa gcc gcc caa aac gtc cac aac cag gaa ggc 1101Ala Gln Ala Ala Glu Ala Ala Gln Asn Val His Asn Gln Glu Gly
355 360 365taagcaggat cctcatgact gct 1124<210>266<211>367<212>PRT<213>谷氨酸棒杆菌<400>266Thr Asp His Thr Leu Ser Ala Leu Leu Asp Ala His Val Glu Val Pro1 5 10 15Thr Ala Val Thr Val Leu Thr Met Arg Leu Asp Asp Pro Thr Gly Tyr
20 25 30Gly Arg Ile Val Arg Asn Glu Glu Gly Glu Val Thr Ala Ile Val Glu
35 40 45Gln Lys Asp Ala Ser Ala Glu Val Gln Ala Ile Asp Glu Val Asn Ser
50 55 60Gly Val Phe Ala Phe Asp Ala Ala Ile Leu Arg Ser Ala Leu Ala Glu65 70 75 80Leu Lys Ser Asp Asn Ala Gln Gly Glu Leu Tyr Leu Thr Asp Val Leu
85 90 95Gly Ile Ala Arg Gly Glu Gly His Pro Val Arg Ala His Thr Ala Ala
100 105 110Asp Ala Arg Glu Leu Ala Gly Val Asn Asp Arg Val Gln Leu Ala Glu
115 120 125Ala Gly Ala Glu Leu Asn Arg Arg Thr Val Ile Ala Ala Met Arg Gly
130 135 140Gly Ala Thr Ile Val Asp Pro Ala Thr Thr Trp Ile Asp Val Glu Val145 150 155 160Ser Ile Gly Arg Asp Val Ile Ile His Pro Gly Thr Gln Leu Lys Gly
165 170 175Glu Thr Val Ile Gly Asp Arg Val Glu Val Gly Pro Asp Thr Thr Leu
180 185 190Thr Asn Met Thr Ile Gly Asp Gly Ala Ser Val Ile Arg Thr His Gly
195 200 205Phe Asp Ser Thr Ile Gly Glu Asn Ala Thr Val Gly Pro Phe Thr Tyr
210 215 220Ile Arg Pro Gly Thr Thr Leu Gly Pro Glu Gly Lys Leu Gly Gly Phe225 230 235 240Val Glu Thr Lys Lys Ala Thr Ile Gly Arg Gly Ser Lys Val Pro His
245 250 255Leu Thr Tyr Val Gly Asp Ala Thr Ile Gly Glu Glu Ser Asn Ile Gly
260 265 270Ala Ser Ser Val Phe Val Asn Tyr Asp Gly Glu Asn Lys His His Thr
275 280 285Thr Ile Gly Ser His Val Arg Thr Gly Ser Asp Thr Met Phe Ile Ala
290 295 300Pro Val Thr Val Gly Asp Gly Ala Tyr Ser Gly Ala Gly Thr Val Ile305 310 315 320Lys Asp Asp Val Pro Pro Gly Ala Leu Ala Val Ser Gly Gly Arg Gln
325 330 335Arg Asn Ile Glu Gly Trp Val Gln Lys Lys Arg Pro Gly Thr Ala Ala
340 345 350Ala Gln Ala Ala Glu Ala Ala Gln Asn Val His Asn Gln Glu Gly
355 360 365<210>267<211>981<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(958)<223>RXA01259<400>267aagagaatta tttctaaaat tcggtatcgt ctaagaaatg agtttgccaa tagctcagca 60tcaaaatgct gtaaaaactg tcgtggtacc agctgcagga atg gga aca cgg ttc 115
Met Gly Thr Arg Phe
1 5ctt cct gca acg aag aca att cca aag gag ctt ctt cct gta gtt gat 163Leu Pro Ala Thr Lys Thr Ile Pro Lys Glu Leu Leu Pro Val Val Asp
10 15 20acc ccg ggt att gaa ctt gtt gcc aaa gag gct gct gat ctt ggt gca 211Thr Pro Gly Ile Glu Leu Val Ala Lys Glu Ala Ala Asp Leu Gly Ala
25 30 35act cgg tta gca att atc act gct ccg aac aaa gac gga att ctt aaa 259Thr Arg Leu Ala Ile Ile Thr Ala Pro Asn Lys Asp Gly Ile Leu Lys
40 45 50cac ttc gag gag ttc cct gag ctt gag gca act ctt gag gct cgc ggt 307His Phe Glu Glu Phe Pro Glu Leu Glu Ala Thr Leu Glu Ala Arg Gly
55 60 65aag act gat caa ctg aat aaa gtt cga gca gct cga gaa ttg att gca 355Lys Thr Asp Gln Leu Asn Lys Val Arg Ala Ala Arg Glu Leu Ile Ala70 75 80 85aca gtt cca gtg gtt caa gaa aag cca ttg ggg ctt ggt cac gct gtt 403Thr Val Pro Val Val Gln Glu Lys Pro Leu Gly Leu Gly His Ala Val
90 95 100ggc ctt gct gag tct gtg ctc gat gat gat gaa gat gtt gtg gct gtc 451Gly Leu Ala Glu Ser Val Leu Asp Asp Asp Glu Asp Val Val Ala Val
105 110 115atg ctg cca gac gat ttg gtg ctg cca ttt ggt gtg acc gag aga atg 499Met Leu Pro Asp Asp Leu Val Leu Pro Phe Gly Val Thr Glu Arg Met
120 125 130gca gaa gtt cgc gct aag ttt ggc gga tct gtt ctt gca gca att gag 547Ala Glu Val Arg Ala Lys Phe Gly Gly Ser Val Leu Ala Ala Ile Glu
135 140 145gtg gct gaa gat gaa gtc tca aat tac gga gta ttt aag ctc ggt gaa 595Val Ala Glu Asp Glu Val Ser Asn Tyr Gly Val Phe Lys Leu Gly Glu150 155 160 165ctc gat gca gag tcc gaa agt gaa ggc att agg cgt gtt gta gga atg 643Leu Asp Ala Glu Ser Glu Ser Glu Gly Ile Arg Arg Val Val Gly Met
170 175 180gtt gaa aag cct gcg cct gaa gat gca cca tca agg ttt gcc gca acg 691Val Glu Lys Pro Ala Pro Glu Asp Ala Pro Ser Arg Phe Ala Ala Thr
185 190 195ggc cgt tat cta ctt gat cga gct att ttt gat gca ctg cgt cga att 739Gly Arg Tyr Leu Leu Asp Arg Ala Ile Phe Asp Ala Leu Arg Arg Ile
200 205 210gag cct ggt gct ggt gga gaa ctg caa tta aca gat gcc atc gca tta 787Glu Pro Gly Ala Gly Gly Glu Leu Gln Leu Thr Asp Ala Ile Ala Leu
215 220 225ttg atc gaa gaa ggc cat ccg gta cac att gtg gtt cat gaa gga aag 835Leu Ile Glu Glu Gly His Pro Val His Ile Val Val His Glu Gly Lys230 235 240 245cgc cat gac ctt ggt aat cca gct ggg tac att cct gct gtt gtg tac 883Arg His Asp Leu Gly Asn Pro Ala Gly Tyr Ile Pro Ala Val Val Tyr
250 255 260ttc gga ctt cgt cat gca gag tac ggt tcc aag att cac cgt gcg gtg 931Phe Gly Leu Arg His Ala Glu Tyr Gly Ser Lys Ile His Arg Ala Val
265 270 275aag gaa ata ctc gct gag ttt gaa tct taaaaaggaa accgccttcc 978Lys Glu Ile Leu Ala Glu Phe Glu Ser
280 285aca 981<210>268<211>286<212>PRT<213>谷氨酸棒杆菌<400>268Met Gly Thr Arg Phe Leu Pro Ala Thr Lys Thr Ile Pro Lys Glu Leu1 5 10 15Leu Pro Val Val Asp Thr Pro Gly Ile Glu Leu Val Ala Lys Glu Ala
20 25 30Ala Asp Leu Gly Ala Thr Arg Leu Ala Ile Ile Thr Ala Pro Asn Lys
35 40 45Asp Gly Ile Leu Lys His Phe Glu Glu Phe Pro Glu Leu Glu Ala Thr
50 55 60Leu Glu Ala Arg Gly Lys Thr Asp Gln Leu Asn Lys Val Arg Ala Ala65 70 75 80Arg Glu Leu Ile Ala Thr Val Pro Val Val Gln Glu Lys Pro Leu Gly
85 90 95Leu Gly His Ala Val Gly Leu Ala Glu Ser Val Leu Asp Asp Asp Glu
100 105 110Asp Val Val Ala Val Met Leu Pro Asp Asp Leu Val Leu Pro Phe Gly
115 120 125Val Thr Glu Arg Met Ala Glu Val Arg Ala Lys Phe Gly Gly Ser Val
130 135 140Leu Ala Ala Ile Glu Val Ala Glu Asp Glu Val Ser Asn Tyr Gly Val145 150 155 160Phe Lys Leu Gly Glu Leu Asp Ala Glu Ser Glu Ser Glu Gly Ile Arg
165 170 175Arg Val Val Gly Met Val Glu Lys Pro Ala Pro Glu Asp Ala Pro Ser
180 185 190Arg Phe Ala Ala Thr Gly Arg Tyr Leu Leu Asp Arg Ala Ile Phe Asp
195 200 205Ala Leu Arg Arg Ile Glu Pro Gly Ala Gly Gly Glu Leu Gln Leu Thr
210 215 220Asp Ala Ile Ala Leu Leu Ile Glu Glu Gly His Pro Val His Ile Val225 230 235 240Val His Glu Gly Lys Arg His Asp Leu Gly Asn Pro Ala Gly Tyr Ile
245 250 255Pro Ala Val Val Tyr Phe Gly Leu Arg His Ala Glu Tyr Gly Ser Lys
260 265 270Ile His Arg Ala Val Lys Glu Ile Leu Ala Glu Phe Glu Ser
275 280 285<210>269<211>526<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(526)<223>RXA02028<400>269tgcgcagttc ctgcttagtt tggctcataa atctaaggat aaccgttatt ttcggagggg 60tacgacgatt ggggttgcgg gggcaggtac tcttggttcc atg agt ttg cct atc 115
Met Ser Leu Pro Ile
1 5gat gag cac gtg aac gcg gtt aaa acc gtc gta gtg cct gct gca gga 163Asp Glu His Val Asn Ala Val Lys Thr Val Val Val Pro Ala Ala Gly
10 15 20ctg gga acc cga ttc ctt ccg gcc acc aaa acc gta ccc aag gag ttg 211Leu Gly Thr Arg Phe Leu Pro Ala Thr Lys Thr Val Pro Lys Glu Leu
25 30 35ctg ccg gtt gtc gat acc cea ggt att gag ctg att gct gct gag gct 259Leu Pro Val Val Asp Thr Pro Gly Ile Glu Leu Ile Ala Ala Glu Ala
40 45 50gcc gaa ctt ggt gcg acc agg ctg gcg atc atc act gcg cca aac aaa 307Ala Glu Leu Gly Ala Thr Arg Leu Ala Ile Ile Thr Ala Pro Asn Lys
55 60 65gct ggg gta ctt gca cac ttt gag cgt tct tct gaa ttg gaa gaa acg 355Ala Gly Val Leu Ala His Phe Glu Arg Ser Ser Glu Leu Glu Glu Thr70 75 80 85ctg atg gag cgt ggc aag act gac cag gtg gag ata atc cgc cgc gcc 403Leu Met Glu Arg Gly Lys Thr Asp Gln Val Glu Ile Ile Arg Arg Ala
90 95 100gcc gat tta atc aag gca gtt cca gta acc cag gac aag ccg ctg ggg 451Ala Asp Leu Ile Lys Ala Val Pro Val Thr Gln Asp Lys Pro Leu Gly
105 110 115cta ggt cat gct gtt ggt ttg gct gag tct gtg ttg gat gat gat gaa 499Leu Gly His Ala Val Gly Leu Ala Glu Ser Val Leu Asp Asp Asp Glu
120 125 130gat gtc gta gcg gtg atg ttg ccg cac 526Asp Val Val Ala Val Met Leu Pro His
135 140<210>270<211>142<212>PRT<213>谷氨酸棒杆菌<400>270Met Ser Leu Pro Ile Asp Glu His Val Asn Ala Val Lys Thr Val Val1 5 10 15Val Pro Ala Ala Gly Leu Gly Thr Arg Phe Leu Pro Ala Thr Lys Thr
20 25 30Val Pro Lys Glu Leu Leu Pro Val Val Asp Thr Pro Gly Ile Glu Leu
35 40 45Ile Ala Ala Glu Ala Ala Glu Leu Gly Ala Thr Arg Leu Ala Ile Ile
50 55 60Thr Ala Pro Asn Lys Ala Gly Val Leu Ala His Phe Glu Arg Ser Ser65 70 75 80Glu Leu Glu Glu Thr Leu Met Glu Arg Gly Lys Thr Asp Gln Val Glu
85 90 95Ile Ile Arg Arg Ala Ala Asp Leu Ile Lys Ala Val Pro Val Thr Gln
100 105 110Asp Lys Pro Leu Gly Leu Gly His Ala Val Gly Leu Ala Glu Ser Val
115 120 125Leu Asp Asp Asp Glu Asp Val Val Ala Val Met Leu Pro His
130 135 140<210>271<211>1284<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1261)<223>RXA01262<400>271tatactcgtc aagggccttc gataaaacaa agacaatttt cccccgacgg gacaatctga 60aaacttgctg tatcaataaa acacgaaaag gaatactttt atg aaa att gcc gtc 115
Met Lys Ile Ala Val
1 5gca ggg ctc gga tat gtt ggg ctt tca aat gca gct ctc ctc tct aaa 163Ala Gly Leu Gly Tyr Val Gly Leu Ser Asn Ala Ala Leu Leu Ser Lys
10 15 20aat cat aaa gtt gtt gca gtt gac att gat gaa gaa cga gtg aaa cta 211Asn His Lys Val Val Ala Val Asp Ile Asp Glu Glu Arg Val Lys Leu
25 30 35gtt caa gaa ttt cgt tcg cca att gtc gat agc gat ctc gaa gaa tat 259Val Gln Glu Phe Arg Ser Pro Ile Val Asp Ser Asp Leu Glu Glu Tyr
40 45 50ctg tcc act aag cct caa aac tta act gcc aca acg gac gcc gaa gcc 307Leu Ser Thr Lys Pro Gln Asn Leu Thr Ala Thr Thr Asp Ala Glu Ala
55 60 65gct tac aaa ggc gca gat ttt att gtt att gca acg cca act aat tac 355Ala Tyr Lys Gly Ala Asp Phe Ile Val Ile Ala Thr Pro Thr Asn Tyr70 75 80 85gac cca gag tca aac ttt ttt gat act tcc agc gtt gag tcc gta att 403Asp Pro Glu Ser Asn Phe Phe Asp Thr Ser Ser Val Glu Ser Val Ile
90 95 100gag ata gtc ctt aag gtt tct cct gga tcc aca atc gta att aaa tcg 451Glu Ile Val Leu Lys Val Ser Pro Gly Ser Thr Ile Val Ile Lys Ser
105 110 115act atc cct gtt ggt ttt aca tcg gaa cta cgc att aag cat eca gaa 499Thr Ile Pro Val Gly Phe Thr Ser Glu Leu Arg Ile Lys His Pro Glu
120 125 130gct tcg att att ttt tca cct gag ttc ctg cgt gaa ggc cga gca ttc 547Ala Ser Ile Ile Phe Ser Pro Glu Phe Leu Arg Glu Gly Arg Ala Phe
135 140 145tac gac aat ctc tac cca tcc aga gtt gtc gtt ggt gat cgc agt cct 595Tyr Asp Asn Leu Tyr Pro Ser Arg Val Val Val Gly Asp Arg Ser Pro150 155 160 165ctg ggg gaa gaa ttt gcg act ctg tta gct gag ggg gca aaa gaa aag 643Leu Gly Glu Glu Phe Ala Thr Leu Leu Ala Glu Gly Ala Lys Glu Lys
170 175 180cct ccg att cta ctt acg gac tca act gag gca gag gcg att aaa tta 691Pro Pro Ile Leu Leu Thr Asp Ser Thr Glu Ala Glu Ala Ile Lys Leu
185 190 195ttt tct aat aca tat ctt gca ctg cga gtt gct ttt ttc aac gaa ctg 739Phe Ser Asn Thr Tyr Leu Ala Leu Arg Val Ala Phe Phe Asn Glu Leu
200 205 210gat act tat gcg tct gtt cga agc ttg gat act aag cag att att gaa 787Asp Thr Tyr Ala Ser Val Arg Ser Leu Asp Thr Lys Gln Ile Ile Glu
215 220 225ggg gta ggg ctc gat cca cgt att gga tct cat tac aat aat cct tca 835Gly Val Gly Leu Asp Pro Arg Ile Gly Ser His Tyr Asn Asn Pro Ser230 235 240 245ttt gga tat ggc gga tat tgt ctt ccg aaa gat acg aaa cag ctt ctc 883Phe Gly Tyr Gly Gly Tyr Cys Leu Pro Lys Asp Thr Lys Gln Leu Leu
250 255 260gcc aac tat aag gat gtc ccg cag aat cta atc tct gca gta gtc caa 931Ala Asn Tyr Lys Asp Val Pro Gln Asn Leu Ile Ser Ala Val Val Gln
265 270 275gca aat aag act cgt aag gac ttt att gca gag gat atc ctc agt aaa 979Ala Asn Lys Thr Arg Lys Asp Phe Ile Ala Glu Asp Ile Leu Ser Lys
280 285 290tca cct act gta gtt gga att tac cgc ctt gta atg aag tct gga tca 1027Ser Pro Thr Val Val Gly Ile Tyr Arg Leu Val Met Lys Ser Gly Ser
295 300 305gat aac ttt cgt tct tct tct att caa gga gtc atg aaa cga att aag 1075Asp Asn Phe Arg Ser Ser Ser Ile Gln Gly Val Met Lys Arg Ile Lys310 315 320 325gcc aag gga atc gaa att gta gta ttt gaa ccg aat ctc gga gaa gaa 1123Ala Lys Gly Ile Glu Ile Val Val Phe Glu Pro Asn Leu Gly Glu Glu
330 335 340act ttc tac aat tcg aag atc ctt aat gac atc gaa gag ttt aag gat 1171Thr Phe Tyr Asn Ser Lys Ile Leu Asn Asp Ile Glu Glu Phe Lys Asp
345 350 355tac tgc gac atc att att gca aat cgt cca acc gat gag ctt tct gat 1219Tyr Cys Asp Ile Ile Ile Ala Asn Arg Pro Thr Asp Glu Leu Ser Asp
360 365 370gta cca gaa aaa gtt tat aca cgt gat att ttc cag cgt gac 1261Val Pro Glu Lys Val Tyr Thr Arg Asp Ile Phe Gln Arg Asp
375 380 385taagtggaaa gaatcttttg ttg 1284<210>272<211>387<212>PRT<213>谷氨酸棒杆菌<400>272Met Lys Ile Ala Val Ala Gly Leu Gly Tyr Val Gly Leu Ser Asn Ala1 5 10 15Ala Leu Leu Ser Lys Asn His Lys Val Val Ala Val Asp Ile Asp Glu
20 25 30Glu Arg Val Lys Leu Val Gln Glu Phe Arg Ser Pro Ile Val Asp Ser
35 40 45Asp Leu Glu Glu Tyr Leu Ser Thr Lys Pro Gln Asn Leu Thr Ala Thr
50 55 60Thr Asp Ala Glu Ala Ala Tyr Lys Gly Ala Asp Phe Ile Val Ile Ala65 70 75 80Thr Pro Thr Asn Tyr Asp Pro Glu Ser Asn Phe Phe Asp Thr Ser Ser
85 90 95Val Glu Ser Val Ile Glu Ile Val Leu Lys Val Ser Pro Gly Ser Thr
100 105 110Ile Val Ile Lys Ser Thr Ile Pro Val Gly Phe Thr Ser Glu Leu Arg
115 120 125Ile Lys His Pro Glu Ala Ser Ile Ile Phe Ser Pro Glu Phe Leu Arg
130 135 140Glu Gly Arg Ala Phe Tyr Asp Asn Leu Tyr Pro Ser Arg Val Val Val145 150 155 160Gly Asp Arg Ser Pro Leu Gly Glu Glu Phe Ala Thr Leu Leu Ala Glu
165 170 175Gly Ala Lys Glu Lys Pro Pro Ile Leu Leu Thr Asp Ser Thr Glu Ala
180 185 190Glu Ala Ile Lys Leu Phe Ser Asn Thr Tyr Leu Ala Leu Arg Val Ala
195 200 205Phe Phe Asn Glu Leu Asp Thr Tyr Ala Ser Val Arg Ser Leu Asp Thr
210 215 220Lys Gln Ile Ile Glu Gly Val Gly Leu Asp Pro Arg Ile Gly Ser His225 230 235 240Tyr Asn Asn Pro Ser Phe Gly Tyr Gly Gly Tyr Cys Leu Pro Lys Asp
245 250 255Thr Lys Gln Leu Leu Ala Asn Tyr Lys Asp Val Pro Gln Asn Leu Ile
260 265 270Ser Ala Val Val Gln Ala Asn Lys Thr Arg Lys Asp Phe Ile Ala Glu
275 280 285Asp Ile Leu Ser Lys Ser Pro Thr Val Val Gly Ile Tyr Arg Leu Val
290 295 300Met Lys Ser Gly Ser Asp Asn Phe Arg Ser Ser Ser Ile Gln Gly Val305 310 315 320Met Lys Arg Ile Lys Ala Lys Gly Ile Glu Ile Val Val Phe Glu Pro
325 330 335Asn Leu Gly Glu Glu Thr Phe Tyr Asn Ser Lys Ile Leu Asn Asp Ile
340 345 350Glu Glu Phe Lys Asp Tyr Cys Asp Ile Ile Ile Ala Asn Arg Pro Thr
355 360 365Asp Glu Leu Ser Asp Val Pro Glu Lys Val Tyr Thr Arg Asp Ile Phe
370 375 380Gln Arg Asp385<210>273<211>1209<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1186)<223>RXA01377<400>273tgaaatggat ttgctgcggc cccggaatta cccttttcgc ggccgtcatc aaatttgtac 60ccccttaaag acaccctaaa cacgagtgaa ataggaacac atg act tta act gac 115
Met Thr Leu Thr Asp
1 5aac agc aaa aac gtt gat gct gtc atc ttg gtc ggt ggc aaa ggt acc 163Asn Ser Lys Asn Val Asp Ala Val Ile Leu Val Gly Gly Lys Gly Thr
10 15 20cga ctg cgc ccc ctg acc gtc aat act cca aag cca atg ctg cca act 211Arg Leu Arg Pro Leu Thr Val Asn Thr Pro Lys Pro Met Leu Pro Thr
25 30 35gct ggc cac cca ttc ttg acc cac ctt ttg gcc cgc atc aag gcc gca 259Ala Gly His Pro Phe Leu Thr His Leu Leu Ala Arg Ile Lys Ala Ala
40 45 50ggc atc aca cac gtc gtg ctg gga acg tca ttc aaa gct gaa gtc ttc 307Gly Ile Thr His Val Val Leu Gly Thr Ser Phe Lys Ala Glu Val Phe
55 60 65gag gaa tac ttc gga gat ggc tcc gaa atg ggc ttg gaa att gaa tat 355Glu Glu Tyr Phe Gly Asp Gly Ser Glu Met Gly Leu Glu Ile Glu Tyr70 75 80 85gtc gtc gag gat cag cct ttg ggc act ggt ggt ggc atc cga aac gtc 403Val Val Glu Asp Gln Pro Leu Gly Thr Gly Gly Gly Ile Arg Asn Val
90 95 100tac gac aag ctg cgt cac gat act gcg att gtg ttc aac ggc gat gtg 451Tyr Asp Lys Leu Arg His Asp Thr Ala Ile Val Phe Asn Gly Asp Val
105 110 115ctc tcc ggt gcg gat ctc aac agc att ctg gac acc cac cgc gaa aag 499Leu Ser Gly Ala Asp Leu Asn Ser Ile Leu Asp Thr His Arg Glu Lys
120 125 130gac gca gat ctg acc atg cat ctc gtg cgc gta gct aac cct cgt gcg 547Asp Ala Asp Leu Thr Met His Leu Val Arg Val Ala Asn Pro Arg Ala
135 140 145ttt ggt tgc gtc ccc acc gat gag gat ggt cgc gtc agc gaa ttc ctt 595Phe Gly Cys Val Pro Thr Asp Glu Asp Gly Arg Val Ser Glu Phe Leu150 155 160 165gaa aag acc gaa gat cca cca acc gat cag atc aac gcc ggc tgc tac 643Glu Lys Thr Glu Asp Pro Pro Thr Asp Gln Ile Asn Ala Gly Cys Tyr
170 175 180gtg ttc aag aag gaa ctc atc gag cag atc ccg gca ggc cga gca gtt 691Val Phe Lys Lys Glu Leu Ile Glu Gln Ile Pro Ala Gly Arg Ala Val
185 190 195tcc gtc gag cgc gaa acc ttc cct cag ctg ttg gaa gaa ggc aag cga 739Ser Val Glu Arg Glu Thr Phe Pro Gln Leu Leu Glu Glu Gly Lys Arg
200 205 210gtc ttc ggc cac gtc gac gct tcc tac tgg cgc gac atg ggc acc cca 787Val Phe Gly His Val Asp Ala Ser Tyr Trp Arg Asp Met Gly Thr Pro
215 220 225agc gac ttc gtc cgc ggc tcg gct gac ctg gtc cgc ggc att gcg tac 835Ser Asp Phe Val Arg Gly Ser Ala Asp Leu Val Arg Gly Ile Ala Tyr230 235 240 245tcc cca ttg ctc gaa ggc aaa aca gga gag tcg ctt gtc gac gcc tcc 883Ser Pro Leu Leu Glu Gly Lys Thr Gly Glu Ser Leu Val Asp Ala Ser
250 255 260gcc ggc gtt cgc gac ggc gtc ctg ctg ctc ggc gga acc gta gtc ggc 931Ala Gly Val Arg Asp Gly Val Leu Leu Leu Gly Gly Thr Val Val Gly
265 270 275cgc ggc act gag atc ggt gcc ggc tgc cgc gtt gac aac act gtt att 979Arg Gly Thr Glu Ile Gly Ala Gly Cys Arg Val Asp Asn Thr Val Ile
280 285 290ttc gac ggc gtc acc att gaa cca ggt gcg gtc att gaa aat tcc atc 1027Phe Asp Gly Val Thr Ile Glu Pro Gly Ala Val Ile Glu Asn Ser Ile
295 300 305att tcc tcg gga gca cgc atc ggt gct aat gcg cac atc tcc ggt tgc 1075Ile Ser Ser Gly Ala Arg Ile Gly Ala Asn Ala His Ile Ser Gly Cys310 315 320 325atc att ggc gag ggc gca cag gtt ggt gct cgg tgt gaa ctc aac gca 1123Ile Ile Gly Glu Gly Ala Gln Val Gly Ala Arg Cys Glu Leu Asn Ala
330 335 340ggg atg cgc gtc ttc cca ggc gtt gtg atc cca gac agc gga att cgt 1171Gly Met Arg Val Phe Pro Gly Val Val Ile Pro Asp Ser Gly lle Arg
345 350 355ttt tcg tct gat cag taggcatttt tagccctttt gga 1209Phe Ser Ser Asp Gln
360<210>274<211>362<212>PRT<213>谷氨酸棒杆菌<400>274Met Thr Leu Thr Asp Asn Ser Lys Asn Val Asp Ala Val Ile Leu Val1 5 10 15Gly Gly Lys Gly Thr Arg Leu Arg Pro Leu Thr Val Asn Thr Pro Lys
20 25 30Pro Met Leu Pro Thr Ala Gly His Pro Phe Leu Thr His Leu Leu Ala
35 40 45Arg Ile Lys Ala Ala Gly Ile Thr His Val Val Leu Gly Thr Ser Phe
50 55 60Lys Ala Glu Val Phe Glu Glu Tyr Phe Gly Asp Gly Ser Glu Met Gly65 70 75 80Leu Glu Ile Glu Tyr Val Val Glu Asp Gln Pro Leu Gly Thr Gly Gly
85 90 95Gly Ile Arg Asn Val Tyr Asp Lys Leu Arg His Asp Thr Ala Ile Val
100 105 110Phe Asn Gly Asp Val Leu Ser Gly Ala Asp Leu Asn Ser Ile Leu Asp
115 120 125Thr His Arg Glu Lys Asp Ala Asp Leu Thr Met His Leu Val Arg Val
130 135 140Ala Asn Pro Arg Ala Phe Gly Cys Val Pro Thr Asp Glu Asp Gly Arg145 150 155 160Val Ser Glu Phe Leu Glu Lys Thr Glu Asp Pro Pro Thr Asp Gln Ile
165 170 175Asn Ala Gly Cys Tyr Val Phe Lys Lys Glu Leu Ile Glu Gln Ile Pro
180 185 190Ala Gly Arg Ala Val Ser Val Glu Arg Glu Thr Phe Pro Gln Leu Leu
195 200 205Glu Glu Gly Lys Arg Val Phe Gly His Val Asp Ala Ser Tyr Trp Arg
210 215 220Asp Met Gly Thr Pro Ser Asp Phe Val Arg Gly Ser Ala Asp Leu Val225 230 235 240Arg Gly Ile Ala Tyr Ser Pro Leu Leu Glu Gly Lys Thr Gly Glu Ser
245 250 255Leu Val Asp Ala Ser Ala Gly Val Arg Asp Gly Val Leu Leu Leu Gly
260 265 270Gly Thr Val Val Gly Arg Gly Thr Glu Ile Gly Ala Gly Cys Arg Val
275 280 285Asp Asn Thr Val Ile Phe Asp Gly Val Thr Ile Glu Pro Gly Ala Val
290 295 300Ile Glu Asn Ser Ile Ile Ser Ser Gly Ala Arg Ile Gly Ala Asn Ala305 310 315 320His Ile Ser Gly Cys Ile Ile Gly Glu Gly Ala Gln Val Gly Ala Arg
325 330 335Cys Glu Leu Asn Ala Gly Met Arg Val Phe Pro Gly Val Val Ile Pro
340 345 350Asp Ser Gly Ile Arg Phe Ser Ser Asp Gln
355 360<210>275<211>1350<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1327)<223>RXA02063<400>275accgaaatgg gggcattaaa aggggctatc attcggaccc caaaacgatg tttagacaat 60ttgttaccca gctttcatgc gggatagtta ttttgccttt atg gtt aag ggt gtg 115
Met Val Lys Gly Val
1 5aag ggt aga cca aat gtt cta gca atc gtt ctc gca ggt ggc gag ggc 163Lys Gly Arg Pro Asn Val Leu Ala Ile Val Leu Ala Gly Gly Glu Gly
10 15 20aaa cga ctt ttt ccg ttg acg gag gac cga gct aag cct gcg gtc cca 211Lys Arg Leu Phe Pro Leu Thr Glu Asp Arg Ala Lys Pro Ala Val Pro
25 30 35ttc ggc gga act tac aga ttg atc gac ttt gtt ttg tcg aac ctg gtg 259Phe Gly Gly Thr Tyr Arg Leu Ile Asp Phe Val Leu Ser Asn Leu Val
40 45 50aac tcc gga ttc ctc aag atc gcg gta ctg act cag tac aag tcg cat 307Asn Ser Gly Phe Leu Lys Ile Ala Val Leu Thr Gln Tyr Lys Ser His
55 60 65tca ttg gat agg cat att tca ttg tcg tgg aac gtg tct ggt cca acg 355Ser Leu Asp Arg His Ile Ser Leu Ser Trp Asn Val Ser Gly Pro Thr70 75 80 85ggg cag tac att gct tct gtt cct gcg cag cag cgc ctg ggc aag cga 403Gly Gln Tyr Ile Ala Ser Val Pro Ala Gln Gln Arg Leu Gly Lys Arg
90 95 100tgg ttc act ggt tcc gcg gat gca att ttg cag tct ctg aac ttg atc 451Trp Phe Thr Gly Ser Ala Asp Ala Ile Leu Gln Ser Leu Asn Leu Ile
105 110 115tct gat gag aaa ccg gat tat gtc atc gtt ttc ggc gcg gac cac gtg 499Ser Asp Glu Lys Pro Asp Tyr Val Ile Val Phe Gly Ala Asp His Val
120 125 130tat cgc atg gac cca agc cag atg cta gat gag cac att gca tct ggt 547Tyr Arg Met Asp Pro Ser Gln Met Leu Asp Glu His Ile Ala Ser Gly
135 140 145cgc gcg gtg tct gtg gca ggt att cgc gtt cca cgt gag gaa gca act 595Arg Ala Val Ser Val Ala Gly Ile Arg Val Pro Arg Glu Glu Ala Thr150 155 160 165gcg ttt ggt tgc atc cag tcc gat gtc gac ggc aac ata acc gag ttc 643Ala Phe Gly Cys Ile Gln Ser Asp Val Asp Gly Asn Ile Thr Glu Phe
170 175 180ttg gaa aag cca gct gac cct ccg gga acc cct gat gat cct gac atg 691Leu Glu Lys Pro Ala Asp Pro Pro Gly Thr Pro Asp Asp Pro Asp Met
185 190 195act tac gcg tcg atg ggt aac tac att ttc acc act gaa gcc ctg atc 739Thr Tyr Ala Ser Met Gly Asn Tyr Ile Phe Thr Thr Glu Ala Leu Ile
200 205 210cag gcg ctg aaa gat gat gaa aat aac gag aac agt gat cat gac atg 787Gln Ala Leu Lys Asp Asp Glu Asn Asn Glu Asn Ser Asp His Asp Met
215 220 225ggc gga gac atc att ccg tac ttc gtc tct cgc aat gat gcg cat gtt 835Gly Gly Asp Ile Ile Pro Tyr Phe Val Ser Arg Asn Asp Ala His Val230 235 240 245tat gat ttc tcc gga aac att gtt cct ggc gca act gag cgt gac aag 883Tyr Asp Phe Ser Gly Asn Ile Val Pro Gly Ala Thr Glu Arg Asp Lys
250 255 260ggc tac tgg cgc gac gtc ggt acc att gat gcg ttc tac gag tgc cac 931Gly Tyr Trp Arg Asp Val Gly Thr Ile Asp Ala Phe Tyr Glu Cys His
265 270 275atg gac ctg att tcc gtg cac cca atc ttc aat ctg tat aac tct gag 979Met Asp Leu Ile Ser Val His Pro Ile Phe Asn Leu Tyr Asn Ser Glu
280 285 290tgg cca atc cac acg acc tct gaa ggt aac ttg cct ccg gct aag ttc 1027Trp Pro Ile His Thr Thr Ser Glu Gly Asn Leu Pro Pro Ala Lys Phe
295 300 305gtt cgg ggc ggt atc gcg cag tcg tcg atg gtg tct tca ggt tcc atc 1075Val Arg Gly Gly Ile Ala Gln Ser Ser Met Val Ser Ser Gly Ser Ile310 315 320 325att tct gct ggg act gtt cgc aac tcc gtg ctg tcc aac aac gtt gtc 1123Ile Ser Ala Gly Thr Val Arg Asn Ser Val Leu Ser Asn Asn Val Val
330 335 340gtc gaa gag ggc gca acg gtg gaa ggt gca gtg ctg atg cca ggc gtg 1171Val Glu Glu Gly Ala Thr Val Glu Gly Ala Val Leu Met Pro Gly Val
345 350 355cgc atc ggt aag ggt gct gtt gtc cgc cat gcg att ctg gac aag aac 1219Arg Ile Gly Lys Gly Ala Val Val Arg His Ala Ile Leu Asp Lys Asn
360 365 370gtg gtt gtc cgc gac gga gag ctc atc ggt gtc gac caa gtg cgc gat 1267Val Val Val Arg Asp Gly Glu Leu Ile Gly Val Asp Gln Val Arg Asp
375 380 385gcg cag cgc ttc aag gtg agc gcc ggc ggc gtc gtg gtt gtc ggt aag 1315Ala Gln Arg Phe Lys Val Ser Ala Gly Gly Val Val Val Val Gly Lys390 395 400 405aac cag gta gtc taaacgggaa agggacctta aaa 1350Asn Gln Val Val<210>276<211>409<212>PRT<213>谷氨酸棒杆菌<400>276Met Val Lys Gly Val Lys Gly Arg Pro Asn Val Leu Ala Ile Val Leu1 5 10 15Ala Gly Gly Glu Gly Lys Arg Leu Phe Pro Leu Thr Glu Asp Arg Ala
20 25 30Lys Pro Ala Val Pro Phe Gly Gly Thr Tyr Arg Leu Ile Asp Phe Val
35 40 45Leu Ser Asn Leu Val Asn Ser Gly Phe Leu Lys Ile Ala Val Leu Thr
50 55 60Gln Tyr Lys Ser His Ser Leu Asp Arg His Ile Ser Leu Ser Trp Asn65 70 75 80Val Ser Gly Pro Thr Gly Gln Tyr Ile Ala Ser Val Pro Ala Gln Gln
85 90 95Arg Leu Gly Lys Arg Trp Phe Thr Gly Ser Ala Asp Ala Ile Leu Gln
100 105 110Ser Leu Asn Leu Ile Ser Asp Glu Lys Pro Asp Tyr Val Ile Val Phe
115 120 125Gly Ala Asp His Val Tyr Arg Met Asp Pro Ser Gln Met Leu Asp Glu
130 135 140His Ile Ala Ser Gly Arg Ala Val Ser Val Ala Gly Ile Arg Val Pro145 150 155 160Arg Glu Glu Ala Thr Ala Phe Gly Cys Ile Gln Ser Asp Val Asp Gly
165 170 175Asn Ile Thr Glu Phe Leu Glu Lys Pro Ala Asp Pro Pro Gly Thr Pro
180 185 190Asp Asp Pro Asp Met Thr Tyr Ala Ser Met Gly Asn Tyr Ile Phe Thr
195 200 205Thr Glu Ala Leu Ile Gln Ala Leu Lys Asp Asp Glu Asn Asn Glu Asn
210 215 220Ser Asp His Asp Met Gly Gly Asp Ile Ile Pro Tyr Phe Val Ser Arg225 230 235 240Asn Asp Ala His Val Tyr Asp Phe Ser Gly Asn Ile Val Pro Gly Ala
245 250 255Thr Glu Arg Asp Lys Gly Tyr Trp Arg Asp Val Gly Thr Ile Asp Ala
260 265 270Phe Tyr Glu Cys His Met Asp Leu Ile Ser Val His Pro Ile Phe Asn
275 280 285Leu Tyr Asn Ser Glu Trp Pro Ile His Thr Thr Ser Glu Gly Asn Leu
290 295 300Pro Pro Ala Lys Phe Val Arg Gly Gly Ile Ala Gln Ser Ser Met Val305 310 315 320Ser Ser Gly Ser Ile Ile Ser Ala Gly Thr Val Arg Asn Ser Val Leu
325 330 335Ser Asn Asn Val Val Val Glu Glu Gly Ala Thr Val Glu Gly Ala Val
340 345 350Leu Met Pro Gly Val Arg Ile Gly Lys Gly Ala Val Val Arg His Ala
355 360 365Ile Leu Asp Lys Asn Val Val Val Arg Asp Gly Glu Leu Ile Gly Val
370 375 380Asp Gln Val Arg Asp Ala Gln Arg Phe Lys Val Ser Ala Gly Gly Val385 390 395 400Val Val Val Gly Lys Asn Gln Val Val
405<210>277<211>903<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(880)<223>RXN00014<400>277catcaaagtg accgccggcg gcgtcgaatg gtccgttgca ggaaacgcgg aagcagttag 60tgagatctcc gaaactttaa gcgcactaga ctaacaacac atg agc aaa tat gca 115
Met Ser Lys Tyr Ala
1 5gac gat tta gcc tta gcc ctc gaa ctt gcc gaa ctt gcc gat tcc atc 163Asp Asp Leu Ala Leu Ala Leu Glu Leu Ala Glu Leu Ala Asp Ser Ile
10 15 20acc ctc gac cgc ttc gaa gcc tct gac ctg gaa gta tcc tcc aag cca 211Thr Leu Asp Arg Phe Glu Ala Ser Asp Leu Glu Val Ser Ser Lys Pro
25 30 35gac atg act ccc gtc agc gat gcc gac ctg gcg acc gaa gaa gca ctc 259Asp Met Thr Pro Val Ser Asp Ala Asp Leu Ala Thr Glu Glu Ala Leu
40 45 50cgt gag aaa atc gcc acc gcc cgc ccc gcc gac tcc atc ctc ggt gaa 307Arg Glu Lys Ile Ala Thr Ala Arg Pro Ala Asp Ser Ile Leu Gly Glu
55 60 65gaa ttc ggt ggc gac gta gaa ttc agc ggc cgc cag tgg atc atc gac 355Glu Phe Gly Gly Asp Val Glu Phe Ser Gly Arg Gln Trp Ile Ile Asp70 75 80 85ccc atc gac ggc acc aaa aac tac gtc cgc ggc gtc ccc gta tgg gca 403Pro Ile Asp Gly Thr Lys Asn Tyr Val Arg Gly Val Pro Val Trp Ala
90 95 100acc ctg atc gcg ctg ctc gac aac ggc aaa ccc gtc gca ggt gtc atc 451Thr Leu Ile Ala Leu Leu Asp Asn Gly Lys Pro Val Ala Gly Val Ile
105 110 115tcc gca ccc gca ctg gct agg cgt tgg tgg gca tcc gaa ggg gcc ggc 499Ser Ala Pro Ala Leu Ala Arg Arg Trp Trp Ala Ser Glu Gly Ala Gly
120 125 130gca tgg cgc acc ttc aac ggc agc tcc cca cgc aaa ctg tcc gtg tcc 547Ala Trp Arg Thr Phe Asn Gly Ser Ser Pro Arg Lys Leu Ser Val Ser
135 140 145cag gtg tcc aag ctt gac gac gcc tcc ctc tcc ttc tcc tcc ctc tcc 595Gln Val Ser Lys Leu Asp Asp Ala Ser Leu Ser Phe Ser Ser Leu Ser150 155 160 165ggc tgg gcc gaa cga gat ttg cgc gat cag ttc gtc tcc cta act gat 643Gly Trp Ala Glu Arg Asp Leu Arg Asp Gln Phe Val Ser Leu Thr Asp
170 175 180acc acc tgg cga ctc cgc ggc tac ggc gac ttc ttc tcc tac tgc ctc 691Thr Thr Trp Arg Leu Arg Gly Tyr Gly Asp Phe Phe Ser Tyr Cys Leu
185 190 195gtc gcc gaa ggt gcc gtc gat atc gcc gct gaa cca gaa gtc agc ctc 739Val Ala Glu Gly Ala Val Asp Ile Ala Ala Glu Pro Glu Val Ser Leu
200 205 210tgg gat ctt gct ccc ctg tcc atc ctg gtc acc gaa gcc gga gga aag 787Trp Asp Leu Ala Pro Leu Ser Ile Leu Val Thr Glu Ala Gly Gly Lys
215 220 225ttc acc tca ctg gct ggc gtc gat gga cca cac ggt ggc gat gca gta 835Phe Thr Ser Leu Ala Gly Val Asp Gly Pro His Gly Gly Asp Ala Val230 235 240 245gcc acc aac ggc atc ctg cac gat gag acg ctg gat cgt tta aaa 880Ala Thr Asn Gly Ile Leu His Asp Glu Thr Leu Asp Arg Leu Lys
250 255 260tagactcccg ggttttgctt ggt 903<210>278<211>260<212>PRT<213>谷氨酸棒杆菌<400>278Met Ser Lys Tyr Ala Asp Asp Leu Ala Leu Ala Leu Glu Leu Ala Glu1 5 10 15Leu Ala Asp Ser Ile Thr Leu Asp Arg Phe Glu Ala Ser Asp Leu Glu
20 25 30Val Ser Ser Lys Pro Asp Met Thr Pro Val Ser Asp Ala Asp Leu Ala
35 40 45Thr Glu Glu Ala Leu Arg Glu Lys Ile Ala Thr Ala Arg Pro Ala Asp
50 55 60Ser Ile Leu Gly Glu Glu Phe Gly Gly Asp Val Glu Phe Ser Gly Arg65 70 75 80Gln Trp Ile Ile Asp Pro Ile Asp Gly Thr Lys Asn Tyr Val Arg Gly
85 90 95Val Pro Val Trp Ala Thr Leu Ile Ala Leu Leu Asp Asn Gly Lys Pro
100 105 110Val Ala Gly Val Ile Ser Ala Pro Ala Leu Ala Arg Arg Trp Trp Ala
115 120 125Ser Glu Gly Ala Gly Ala Trp Arg Thr Phe Asn Gly Ser Ser Pro Arg
130 135 140Lys Leu Ser Val Ser Gln Val Ser Lys Leu Asp Asp Ala Ser Leu Ser145 150 155 160Phe Ser Ser Leu Ser Gly Trp Ala Glu Arg Asp Leu Arg Asp Gln Phe
165 170 175Val Ser Leu Thr Asp Thr Thr Trp Arg Leu Arg Gly Tyr Gly Asp Phe
180 185 190Phe Ser Tyr Cys Leu Val Ala Glu Gly Ala Val Asp Ile Ala Ala Glu
195 200 205Pro Glu Val Ser Leu Trp Asp Leu Ala Pro Leu Ser Ile Leu Val Thr
210 215 220Glu Ala Gly Gly Lys Phe Thr Ser Leu Ala Gly Val Asp Gly Pro His225 230 235 240Gly Gly Asp Ala Val Ala Thr Asn Gly Ile Leu His Asp Glu Thr Leu
245 250 255Asp Arg Leu Lys
260<210>279<211>903<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(880)<223>FRXA00014<400>279catcaaagtg accgccggcg gcgtcgaatg gtccgttgca ggaaacgcgg aagcagttag 60tgagatctcc gaaactttaa gcgcactaga ctagcaacac atg agc aaa tat gca 115
Met Ser Lys Tyr Ala
1 5gac gat tta gcc tta gcc ctc gaa ctc gcc gaa ctt gcc gat tcc atc 163Asp Asp Leu Ala Leu Ala Leu Glu Leu Ala Glu Leu Ala Asp Ser Ile
10 15 20acc ctc gac cgc ttc gaa gcc tct gac ctg gaa gta tcc tcc aag cca 211Thr Leu Asp Arg Phe Glu Ala Ser Asp Leu Glu Val Ser Ser Lys Pro
25 30 35gac atg act ccc gtc agc gat gcc gac ctg gcg acc gaa gaa gca ctc 259Asp Met Thr Pro Val Ser Asp Ala Asp Leu Ala Thr Glu Glu Ala Leu
40 45 50cgc gag aaa atc gcc acc gcc cgc ccc gcc gac tcc atc ctc ggt gaa 307Arg Glu Lys Ile Ala Thr Ala Arg Pro Ala Asp Ser Ile Leu Gly Glu
55 60 65gaa ttc ggt ggc gac gta gaa ttc agc ggc cgc cag tgg atc atc gac 355Glu Phe Gly Gly Asp Val Glu Phe Ser Gly Arg Gln Trp Ile Ile Asp70 75 80 85ccc atc gac ggc acc aaa aac tac gtc cgc ggc gtc ccc gta tgg gca 403Pro Ile Asp Gly Thr Lys Asn Tyr Val Arg Gly Val Pro Val Trp Ala
90 95 100acc ctg atc gcg ctg ctc gac aac ggc aaa ccc gtc gca ggt gtc atc 451Thr Leu Ile Ala Leu Leu Asp Asn Gly Lys Pro Val Ala Gly Val Ile
105 110 115tcc gca ccc gca ctg gct agg cgt tgg tgg gca tcc gaa ggg gcc ggc 499Ser Ala Pro Ala Leu Ala Arg Arg Trp Trp Ala Ser Glu Gly Ala Gly
120 125 130gca tgg cgc acc ttc aac ggc agc tcc cca cgc aaa ctg tcc gtg tcc 547Ala Trp Arg Thr Phe Asn Gly Ser Ser Pro Arg Lys Leu Ser Val Ser
135 140 145cag gtg tcc aag ctt gac gac gcc tcc ctc tcc ttc tcc tcc ctc tcc 595Gln Val Ser Lys Leu Asp Asp Ala Ser Leu Ser Phe Ser Ser Leu Ser150 155 160 165ggc tgg gcc gaa cga gat ttg cgc gat cag ttc gtc tcc cta act gat 643Gly Trp Ala Glu Arg Asp Leu Arg Asp Gln Phe Val Ser Leu Thr Asp
170 175 180acc acc tgg cga ctc cgc ggc tac ggc gac ttc ttc tcc tac tgc ctc 691Thr Thr Trp Arg Leu Arg Gly Tyr Gly Asp Phe Phe Ser Tyr Cys Leu
185 190 195gtc gcc gaa ggt gcc gtc gat atc gcc gct gaa cca gaa gtc agc ctc 739Val Ala Glu Gly Ala Val Asp Ile Ala Ala Glu Pro Glu Val Ser Leu
200 205 210tgg gat ctt gct ccc ctg tcc atc ctg gtc acc gaa gcc gga gga aag 787Trp Asp Leu Ala Pro Leu Ser Ile Leu Val Thr Glu Ala Gly Gly Lys
215 220 225ttc acc tca ctg gct ggc gtc gat gga cca cac ggt ggc gat gca gta 835Phe Thr Ser Leu Ala Gly Val Asp Gly Pro His Gly Gly Asp Ala Val230 235 240 245gcc acc aac ggc atc ctg cac gat gag acg ctg gat cgt tta aaa 880Ala Thr Asn Gly Ile Leu His Asp Glu Thr Leu Asp Arg Leu Lys
250 255 260tagactcccg ggttttgctt ggt 903<210>280<211>260<212>PRT<213>谷氨酸棒杆菌<400>280Met Ser Lys Tyr Ala Asp Asp Leu Ala Leu Ala Leu Glu Leu Ala Glu1 5 10 15Leu Ala Asp Ser Ile Thr Leu Asp Arg Phe Glu Ala Ser Asp Leu Glu
20 25 30Val Ser Ser Lys Pro Asp Met Thr Pro Val Ser Asp Ala Asp Leu Ala
35 40 45Thr Glu Glu Ala Leu Arg Glu Lys Ile Ala Thr Ala Arg Pro Ala Asp
50 55 60Ser Ile Leu Gly Glu Glu Phe Gly Gly Asp Val Glu Phe Ser Gly Arg65 70 75 80Gln Trp Ile Ile Asp Pro Ile Asp Gly Thr Lys Asn Tyr Val Arg Gly
85 90 95Val Pro Val Trp Ala Thr Leu Ile Ala Leu Leu Asp Asn Gly Lys Pro
100 105 110Val Ala Gly Val Ile Ser Ala Pro Ala Leu Ala Arg Arg Trp Trp Ala
115 120 125Ser Glu Gly Ala Gly Ala Trp Arg Thr Phe Asn Gly Ser Ser Pro Arg
130 135 140Lys Leu Ser Val Ser Gln Val Ser Lys Leu Asp Asp Ala Ser Leu Ser145 150 155 160Phe Ser Ser Leu Ser Gly Trp Ala Glu Arg Asp Leu Arg Asp Gln Phe
165 170 175Val Ser Leu Thr Asp Thr Thr Trp Arg Leu Arg Gly Tyr Gly Asp Phe
180 185 190Phe Ser Tyr Cys Leu Val Ala Glu Gly Ala Val Asp Ile Ala Ala Glu
195 200 205Pro Glu Val Ser Leu Trp Asp Leu Ala Pro Leu Ser Ile Leu Val Thr
210 215 220Glu Ala Gly Gly Lys Phe Thr Ser Leu Ala Gly Val Asp Gly Pro His225 230 235 240Gly Gly Asp Ala Val Ala Thr Asn Gly Ile Leu His Asp Glu Thr Leu
245 250 255Asp Arg Leu Lys
260<210>281<211>978<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(955)<223>RXA01570<400>281cactgaactc cgttttggat ctcggcaaaa tcgaagccac cggatttagc gcaccgacct 60ggcagacccg cctcaacgac tacctcaagg aactctcaaa gtg aaa ggc atc atc 115
Val Lys Gly Ile Ile
1 5ctc gca ggt ggc tcc ggc acc cgg ctc tac ccc atc acc aag ggc atc 163Leu Ala Gly Gly Ser Gly Thr Arg Leu Tyr Pro Ile Thr Lys Gly Ile
10 15 20tcc aag caa ctg atg ccg att tac gac aaa ccc atg gtc tac tac cca 211Ser Lys Gln Leu Met Pro Ile Tyr Asp Lys Pro Met Val Tyr Tyr Pro
25 30 35ctg acc acg ctc att cag gcc ggc atc aaa gac atc ctg att atc acc 259Leu Thr Thr Leu Ile Gln Ala Gly Ile Lys Asp Ile Leu Ile Ile Thr
40 45 50acc cct gaa gac agc gcc tcc ttt gaa cgc ttg ctt ggc gac ggc tcc 307Thr Pro Glu Asp Ser Ala Ser Phe Glu Arg Leu Leu Gly Asp Gly Ser
55 60 65tcc tgg ggc atc aac ctc acc tac gcc gtc caa ccc tcc ccc gac gga 355Ser Trp Gly Ile Asn Leu Thr Tyr Ala Val Gln Pro Ser Pro Asp Gly70 75 80 85cta gcc caa gca ttc atc atc ggc gag gaa ttc atc ggt gac gac gac 403Leu Ala Gln Ala Phe Ile Ile Gly Glu Glu Phe Ile Gly Asp Asp Asp
90 95 100gtc gcg ttg gtg ctt ggc gat aac atc ttc gac ggc gca caa ctt ggc 451Val Ala Leu Val Leu Gly Asp Asn Ile Phe Asp Gly Ala Gln Leu Gly
105 110 115cac gca cta aag cag tgc tcc aac ccc gac ggt ggc att gtc ttt gct 499His Ala Leu Lys Gln Cys Ser Asn Pro Asp Gly Gly Ile Val Phe Ala
120 125 130tat gag gtc tcc gat cct gag cgt tat ggc gtg gtg gaa ttt gat gct 547Tyr Glu Val Ser Asp Pro Glu Arg Tyr Gly Val Val Glu Phe Asp Ala
135 140 145gct aat aag gcg gtg tct att gaa gaa aag ccc acc gcg cca aaa tcc 595Ala Asn Lys Ala Val Ser Ile Glu Glu Lys Pro Thr Ala Pro Lys Ser150 155 160 165aac ttt gcc gtg gta gga cta tat ttc tac gac aat cgc gtg gtg gac 643Asn Phe Ala Val Val Gly Leu Tyr Phe Tyr Asp Asn Arg Val Val Asp
170 175 180atc gcc aag tca atc aag cct tcc tcg cgt ggc gaa ctg gaa atc acc 691Ile Ala Lys Ser Ile Lys Pro Ser Ser Arg Gly Glu Leu Glu Ile Thr
185 190 195tcc gtt aac gat gcc tac ctc cag caa ggt gct tta act gtg cag cgc 739Ser Val Asn Asp Ala Tyr Leu Gln Gln Gly Ala Leu Thr Val Gln Arg
200 205 210ctg gac cgt ggc gat gtc tgg tta gat acc ggc aca atc gat tcc atg 787Leu Asp Arg Gly Asp Val Trp Leu Asp Thr Gly Thr Ile Asp Ser Met
215 220 225tcc gag gcg tct tcc tat gtt gag gtc ctg caa aaa cgt acc ggc aac 835Ser Glu Ala Ser Ser Tyr Val Glu Val Leu Gln Lys Arg Thr Gly Asn230 235 240 245atc atc gga tcc ccc gaa gtc gct gcg tac cgc gaa ggt ttc atc aca 883Ile Ile Gly Ser Pro Glu Val Ala Ala Tyr Arg Glu Gly Phe Ile Thr
250 255 260gct gaa gaa ctc aca gtg ctt ggt gag gaa ctg aag aaa tca ggc tac 931Ala Glu Glu Leu Thr Val Leu Gly Glu Glu Leu Lys Lys Ser Gly Tyr
265 270 275gga aac tac ctg ctg aga gct ttg taatttacgg tgtggttgtg gag 978Gly Asn Tyr Leu Leu Arg Ala Leu
280 285<210>282<211>285<212>PRT<213>谷氨酸棒杆菌<400>282Val Lys Gly Ile Ile Leu Ala Gly Gly Ser Gly Thr Arg Leu Tyr Pro1 5 10 15Ile Thr Lys Gly Ile Ser Lys Gln Leu Met Pro Ile Tyr Asp Lys Pro
20 25 30Met Val Tyr Tyr Pro Leu Thr Thr Leu Ile Gln Ala Gly Ile Lys Asp
35 40 45Ile Leu Ile Ile Thr Thr Pro Glu Asp Ser Ala Ser Phe Glu Arg Leu
50 55 60Leu Gly Asp Gly Ser Ser Trp Gly Ile Asn Leu Thr Tyr Ala Val Gln65 70 75 80Pro Ser Pro Asp Gly Leu Ala Gln Ala Phe Ile Ile Gly Glu Glu Phe
85 90 95Ile Gly Asp Asp Asp Val Ala Leu Val Leu Gly Asp Asn Ile Phe Asp
100 105 110Gly Ala Gln Leu Gly His Ala Leu Lys Gln Cys Ser Asn Pro Asp Gly
115 120 125Gly Ile Val Phe Ala Tyr Glu Val Ser Asp Pro Glu Arg Tyr Gly Val
130 135 140Val Glu Phe Asp Ala Ala Asn Lys Ala Val Ser Ile Glu Glu Lys Pro145 150 155 160Thr Ala Pro Lys Ser Asn Phe Ala Val Val Gly Leu Tyr Phe Tyr Asp
165 170 175Asn Arg Val Val Asp Ile Ala Lys Ser Ile Lys Pro Ser Ser Arg Gly
180 185 190Glu Leu Glu Ile Thr Ser Val Asn Asp Ala Tyr Leu Gln Gln Gly Ala
195 200 205Leu Thr Val Gln Arg Leu Asp Arg Gly Asp Val Trp Leu Asp Thr Gly
210 215 220Thr Ile Asp Ser Met Ser Glu Ala Ser Ser Tyr Val Glu Val Leu Gln225 230 235 240Lys Arg Thr Gly Asn Ile Ile Gly Ser Pro Glu Val Ala Ala Tyr Arg
245 250 255Glu Gly Phe Ile Thr Ala Glu Glu Leu Thr Val Leu Gly Glu Glu Leu
260 265 270Lys Lys Ser Gly Tyr Gly Asn Tyr Leu Leu Arg Ala Leu
275 280 285<210>283<211>891<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(868)<223>RXA02666<400>283gctcggcgac gaggaagaga agaaggacgc attcgacgac ttcgacgatt ccgacgtgga 60tcttgacgat ctgagcttcg acgacgaaga ttagacgccc atg tcg tct aca cga 115
Met Ser Ser Thr Arg
1 5atc ccc gtc atc gca ctc ctc gcg gcg gcg ggg cgc gga acc cgc ctc 163Ile Pro Val Ile Ala Leu Leu Ala Ala Ala Gly Arg Gly Thr Arg Leu
10 15 20ggc gga ccc atc ccc aaa gca ttc gtc acg ttg cgt gaa cgc aca ctt 211Gly Gly Pro Ile Pro Lys Ala Phe Val Thr Leu Arg Glu Arg Thr Leu
25 30 35tta gag cgc tcg ctc caa gcc atg ctc acc tcc gaa agc gtc gac gaa 259Leu Glu Arg Ser Leu Gln Ala Met Leu Thr Ser Glu Ser Val Asp Glu
40 45 50atc atc atc ctc gtc agc ccc gac atg gaa acc tac gcc cgc gat ttg 307Ile Ile Ile Leu Val Ser Pro Asp Met Glu Thr Tyr Ala Arg Asp Leu
55 60 65ctg cgc aaa cgc ggt ctt ttg aac gac ccc gaa ggg gta cgc gta cgg 355Leu Arg Lys Arg Gly Leu Leu Asn Asp Pro Glu Gly Val Arg Val Arg70 75 80 85ctc gtg cac ggc ggc ggg gag cgc gcg gac tcg gtc tgg gca ggc ctt 403Leu Val His Gly Gly Gly Glu Arg Ala Asp Ser Val Trp Ala Gly Leu
90 95 100cag gca att tcg ctt gac gac gcc acc ccc gat gca att gtc tta atc 451Gln Ala Ile Ser Leu Asp Asp Ala Thr Pro Asp Ala Ile Val Leu Ile
105 110 115cac gac agc gcc cga gcg ctc aca cca ccc ggc atg att gcg cgc gtg 499His Asp Ser Ala Arg Ala Leu Thr Pro Pro Gly Met Ile Ala Arg Val
120 125 130gtg cgc aaa gtc cac gaa ggc gcc acc gca gtc atc cca gta ctg cca 547Val Arg Lys Val His Glu Gly Ala Thr Ala Val Ile Pro Val Leu Pro
135 140 145gta tcg gac acc atc aaa cga gtg tcc cct gat ggc gga gta gtt gtc 595Val Ser Asp Thr Ile Lys Arg Val Ser Pro Asp Gly Gly Val Val Val150 155 160 165gac aca ccc aac cgt gca gaa ctt cgc gcc gtc caa acc cca caa ggc 643Asp Thr Pro Asn Arg Ala Glu Leu Arg Ala Val Gln Thr Pro Gln Gly
170 175 180ttc ctg ctg tcc gaa ctt gtt gca gcg aat gag aaa ttc ttc gcc gac 691Phe Leu Leu Ser Glu Leu Val Ala Ala Asn Glu Lys Phe Phe Ala Asp
185 190 195ccc aac cca ggc ttc atc cca acc gat gac gcc agc ttg atg gaa tgg 739Pro Asn Pro Gly Phe Ile Pro Thr Asp Asp Ala Ser Leu Met Glu Trp
200 205 210tac ggc gca gat gta gtc tgc gta caa ggc gac cca atg gcg ttt aaa 787Tyr Gly Ala Asp Val Val Cys Val Gln Gly Asp Pro Met Ala Phe Lys
215 220 225gta aca acc ccc att gat atg atg ctg gca caa cgc atc acc gac gaa 835Val Thr Thr Pro Ile Asp Met Met Leu Ala Gln Arg Ile Thr Asp Glu230 235 240 245gcc gaa ccc aca ata ttt gag gta cca ggt gac taacccaatc atcccccgcg 888Ala Glu Pro Thr Ile Phe Glu Val Pro Gly Asp
250 255tag 891<210>284<211>256<212>PRT<213>谷氨酸棒杆菌<400>284Met Ser Ser Thr Arg Ile Pro Val Ile Ala Leu Leu Ala Ala Ala Gly1 5 10 15Arg Gly Thr Arg Leu Gly Gly Pro Ile Pro Lys Ala Phe Val Thr Leu
20 25 30Arg Glu Arg Thr Leu Leu Glu Arg Ser Leu Gln Ala Met Leu Thr Ser
35 40 45Glu Ser Val Asp Glu Ile Ile Ile Leu Val Ser Pro Asp Met Glu Thr
50 55 60Tyr Ala Arg Asp Leu Leu Arg Lys Arg Gly Leu Leu Asn Asp Pro Glu65 70 75 80Gly Val Arg Val Arg Leu Val His Gly Gly Gly Glu Arg Ala Asp Ser
85 90 95Val Trp Ala Gly Leu Gln Ala Ile Ser Leu Asp Asp Ala Thr Pro Asp
100 105 110Ala Ile Val Leu Ile His Asp Ser Ala Arg Ala Leu Thr Pro Pro Gly
115 120 125Met Ile Ala Arg Val Val Arg Lys Val His Glu Gly Ala Thr Ala Val
130 135 140Ile Pro Val Leu Pro Val Ser Asp Thr Ile Lys Arg Val Ser Pro Asp145 150 155 160Gly Gly Val Val Val Asp Thr Pro Asn Arg Ala Glu Leu Arg Ala Val
165 170 175Gln Thr Pro Gln Gly Phe Leu Leu Ser Glu Leu Val Ala Ala Asn Glu
180 185 190Lys Phe Phe Ala Asp Pro Asn Pro Gly Phe Ile Pro Thr Asp Asp Ala
195 200 205Ser Leu Met Glu Trp Tyr Gly Ala Asp Val Val Cys Val Gln Gly Asp
210 215 220Pro Met Ala Phe Lys Val Thr Thr Pro Ile Asp Met Met Leu Ala Gln225 230 235 240Arg Ile Thr Asp Glu Ala Glu Pro Thr Ile Phe Glu Val Pro Gly Asp
245 250 255<210>285<211>1056<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1033)<223>RXA00825<400>285cccgttcatg ctgggctttg gtgcggtgat ggcaactatt tgtctgatca ttgtgagttt 60tagtgcacgc cgattctgag aaacaactaa agtgagccac atg cgc aca gta gtt 115
Met Arg Thr Val Val
1 5acc ggc ggt gcc ggc ttc atc gga tcc cat ctc gtt gac ctt ttg atc 163Thr Gly Gly Ala Gly Phe Ile Gly Ser His Leu Val Asp Leu Leu Ile
10 15 20aag gaa ggc cac gag gtc gtt gtg atc gat aac ctc tcc cgc gga cgc 211Lys Glu Gly His Glu Val Val Val Ile Asp Asn Leu Ser Arg Gly Arg
25 30 35ctg gag aat ctc tcc gat gcg gaa gcc acc gga aaa ctc acc ttt gtg 259Leu Glu Asn Leu Ser Asp Ala Glu Ala Thr Gly Lys Leu Thr Phe Val
40 45 50gaa gcc gat ctt ctc gac gtt gat ttc aac gag ttt cta gga acc cac 307Glu Ala Asp Leu Leu Asp Val Asp Phe Asn Glu Phe Leu Gly Thr His
55 60 65aag cct gag gtt att ttc cac ctg gca gcg caa atc gat gtg cgc cac 355Lys Pro Glu Val Ile Phe His Leu Ala Ala Gln Ile Asp Val Arg His70 75 80 85tct gtt gta gat cct ctt cac gac gcc gaa acc aac att ttg tcc acc 403Ser Val Val Asp Pro Leu His Asp Ala Glu Thr Asn Ile Leu Ser Thr
90 95 100atc cgc atc gct gac gct gcc cgc cag cac ggt gtt cgc aag gtt gtc 451Ile Arg Ile Ala Asp Ala Ala Arg Gln His Gly Val Arg Lys Val Val
105 110 115ttt acc tcc tca ggc ggt tcc att tac ggt gag cct tcg gaa ttc cca 499Phe Thr Ser Ser Gly Gly Ser Ile Tyr Gly Glu Pro Ser Glu Phe Pro
120 125 130gtt gat gaa acc gtg cca gtg gat cca cat tcc cct tat gcg gca tcc 547Val Asp Glu Thr Val Pro Val Asp Pro His Ser Pro Tyr Ala Ala Ser
135 140 145aag gtg tcc ggt gaa att tac ctg aac acc ttc cgc cac ctg tac ggc 595Lys Val Ser Gly Glu Ile Tyr Leu Asn Thr Phe Arg His Leu Tyr Gly150 155 160 165tta gac tgt tct cac atc gca ccg gca aat gtt tac ggc cca cgc caa 643Leu Asp Cys Ser His Ile Ala Pro Ala Asn Val Tyr Gly Pro Arg Gln
170 175 180gat cca cac ggt gaa gca gga gtt gtg gcc att ttc gcg ctg cga ctt 691Asp Pro His Gly Glu Ala Gly Val Val Ala Ile Phe Ala Leu Arg Leu
185 190 195ctg gga ggc ctg gac acc aag gta ttc ggc gac ggc gga aac acc cgc 739Leu Gly Gly Leu Asp Thr Lys Val Phe Gly Asp Gly Gly Asn Thr Arg
200 205 210gac tac gtc tac gtc ggt gac gta gtt cgt gct ttc tac ctg gct tct 787Asp Tyr Val Tyr Val Gly Asp Val Val Arg Ala Phe Tyr Leu Ala Ser
215 220 225ggg gaa atc ggt ggg gga gag cgc ttc aac att ggc acc tct gtg gaa 835Gly Glu Ile Gly Gly Gly Glu Arg Phe Asn Ile Gly Thr Ser Val Glu230 235 240 245acc tct gac cgc cag ctg cac acc ctc gtg gcc act gcg gca ggt tcc 883Thr Ser Asp Arg Gln Leu His Thr Leu Val Ala Thr Ala Ala Gly Ser
250 255 260aaa gat gat cct gaa tat gca cct gca cgt ctc ggc gat gtg cca cgc 931Lys Asp Asp Pro Glu Tyr Ala Pro Ala Arg Leu Gly Asp Val Pro Arg
265 270 275agt gca ctc agc ttc ggc aag gcc aaa gag gtg ctt ggt tgg gag cct 979Ser Ala Leu Ser Phe Gly Lys Ala Lys Glu Val Leu Gly Trp Glu Pro
280 285 290gag gtg aac atc gaa caa ggt gtg gcc aag act gtg gag tac ttc cgc 1027Glu Val Asn Ile Glu Gln Gly Val Ala Lys Thr Val Glu Tyr Phe Arg
295 300 305act cac taggggaaaa tccaccacaa atc 1056Thr His310<210>286<211>311<212>PRT<213>谷氨酸棒杆菌<400>286Met Arg Thr Val Val Thr Gly Gly Ala Gly Phe Ile Gly Ser His Leu1 5 10 15Val Asp Leu Leu Ile Lys Glu Gly His Glu Val Val Val Ile Asp Asn
20 25 30Leu Ser Arg Gly Arg Leu Glu Asn Leu Ser Asp Ala Glu Ala Thr Gly
35 40 45Lys Leu Thr Phe Val Glu Ala Asp Leu Leu Asp Val Asp Phe Asn Glu
50 55 60Phe Leu Gly Thr His Lys Pro Glu Val Ile Phe His Leu Ala Ala Gln65 70 75 80Ile Asp Val Arg His Ser Val Val Asp Pro Leu His Asp Ala Glu Thr
85 90 95Asn Ile Leu Ser Thr Ile Arg Ile Ala Asp Ala Ala Arg Gln His Gly
100 105 110Val Arg Lys Val Val Phe Thr Ser Ser Gly Gly Ser Ile Tyr Gly Glu
115 120 125Pro Ser Glu Phe Pro Val Asp Glu Thr Val Pro Val Asp Pro His Ser
130 135 140Pro Tyr Ala Ala Ser Lys Val Ser Gly Glu Ile Tyr Leu Asn Thr Phe145 150 155 160Arg His Leu Tyr Gly Leu Asp Cys Ser His Ile Ala Pro Ala Asn Val
165 170 175Tyr Gly Pro Arg Gln Asp Pro His Gly Glu Ala Gly Val Val Ala Ile
180 185 190Phe Ala Leu Arg Leu Leu Gly Gly Leu Asp Thr Lys Val Phe Gly Asp
195 200 205Gly Gly Asn Thr Arg Asp Tyr Val Tyr Val Gly Asp Val Val Arg Ala
210 215 220Phe Tyr Leu Ala Ser Gly Glu Ile Gly Gly Gly Glu Arg Phe Asn Ile225 230 235 240Gly Thr Ser Val Glu Thr Ser Asp Arg Gln Leu His Thr Leu Val Ala
245 250 255Thr Ala Ala Gly Ser Lys Asp Asp Pro Glu Tyr Ala Pro Ala Arg Leu
260 265 270Gly Asp Val Pro Arg Ser Ala Leu Ser Phe Gly Lys Ala Lys Glu Val
275 280 285Leu Gly Trp Glu Pro Glu Val Asn Ile Glu Gln Gly Val Ala Lys Thr
290 295 300Val Glu Tyr Phe Arg Thr His305 310<210>287<211>1134<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1111)<223>RXA01887<400>287catctttaca ggaaacccct tgacggcatc aatgggtggt atctagtatc tactagaacg 60ttatagtaga acgttctagt aaaacttgga aggatgaaaa atg tca gtc aaa ctt 115
Met Ser Val Lys Leu
1 5gcc ctc atc ggt gct gga cgc atc gga tca aat cac gca cgc ctg atc 163Ala Leu Ile Gly Ala Gly Arg Ile Gly Ser Asn His Ala Arg Leu Ile
10 15 20aca aac cac gtg atc ggc tct gaa ctg gtc gcc gtc gtt gac cca act 211Thr Asn His Val Ile Gly Ser Glu Leu Val Ala Val Val Asp Pro Thr
25 30 35ccc aac gca gaa acc ctc gct gat gaa ttg ggc gcc gtt gcg ttc tct 259Pro Asn Ala Glu Thr Leu Ala Asp Glu Leu Gly Ala Val Ala Phe Ser
40 45 50aac cca gat gac gtc ctg acc cgc gat gac att gac gcg gtt ttg att 307Asn Pro Asp Asp Val Leu Thr Arg Asp Asp Ile Asp Ala Val Leu Ile
55 60 65gct aca cca gca cga acc cac gcg gat ctc gtg gtc aaa gca gcg gca 355Ala Thr Pro Ala Arg Thr His Ala Asp Leu Val Val Lys Ala Ala Ala70 75 80 85gcg ggc aag cac gtg ttt gtg gaa aag ccc atg gcc gtc acc ctc gag 403Ala Gly Lys His Val Phe Val Glu Lys Pro Met Ala Val Thr Leu Glu
90 95 100gac gca gat cgt gcc atc aac gca gca cgc gaa gca aac act gtc ctg 451Asp Ala Asp Arg Ala Ile Asn Ala Ala Arg Glu Ala Asn Thr Val Leu
105 110 115cag gtg ggc ttc aat cgt cgt ttc gcg gca ggt ttc gct gca gca cgc 499Gln Val Gly Phe Asn Arg Arg Phe Ala Ala Gly Phe Ala Ala Ala Arg
120 125 130gca cgc att gac gca ggc gat atc ggc acc cca cag ctg ctt cgt tcc 547Ala Arg Ile Asp Ala Gly Asp Ile Gly Thr Pro Gln Leu Leu Arg Ser
135 140 145gtg acc cgc gat cca gga cca ttc acc gct gac ccc aac aag atc cct 595Val Thr Arg Asp Pro Gly Pro Phe Thr Ala Asp Pro Asn Lys Ile Pro150 155 160 165cag tgg acc atc ttc ctg gaa acc ctc atc cac gat ttc gat gcg ctg 643Gln Trp Thr Ile Phe Leu Glu Thr Leu Ile His Asp Phe Asp Ala Leu
170 175 180tgc tac ctc aac cca ggc gca acc cca gtg gaa gta acc gct cac gct 691Cys Tyr Leu Asn Pro Gly Ala Thr Pro Val Glu Val Thr Ala His Ala
185 190 195gat tgc ctc gtc gtt cca gaa gct gct ggc act ggc ttc ctc gac acc 739Asp Cys Leu Val Val Pro Glu Ala Ala Gly Thr Gly Phe Leu Asp Thr
200 205 210gca gtg gtg act gtc cgt ttt gat aac gga gca att ggt act gca gaa 787Ala Val Val Thr Val Arg Phe Asp Asn Gly Ala Ile Gly Thr Ala Glu
215 220 225gca agc ttc agc gca gcc tat ggt tat gac gtt cgc ggt gaa gtc ttc 835Ala Ser Phe Ser Ala Ala Tyr Gly Tyr Asp Val Arg Gly Glu Val Phe230 235 240 245gga tcc aag ggc atg atg acc gca ggc gac gcg cgc gca acc aac atg 883Gly Ser Lys Gly Met Met Thr Ala Gly Asp Ala Arg Ala Thr Asn Met
250 255 260act ttc tac ggc gct gag ggc atc gcg gct gcc acc tca cgc gcg gat 931Thr Phe Tyr Gly Ala Glu Gly Ile Ala Ala Ala Thr Ser Arg Ala Asp
265 270 275acc gat ctg ctc tcc gat gct tac cga gct gaa ttc caa gct ttc gtc 979Thr Asp Leu Leu Ser Asp Ala Tyr Arg Ala Glu Phe Gln Ala Phe Val
280 285 290gac tcc atc cgt acc aac acc cct tcc aag gtt cca ggc gaa gct gca 1027Asp Ser Ile Arg Thr Asn Thr Pro Ser Lys Val Pro Gly Glu Ala Ala
295 300 305cgc acc gca cta ctc atc gca ctc ggc gcc atc cga agc gta gaa acc 1075Arg Thr Ala Leu Leu Ile Ala Leu Gly Ala Ile Arg Ser Val Glu Thr310 315 320 325ggc gca acc atc aac ctt gct gaa agc atc gag gtt taaccatgac 1121Gly Ala Thr Ile Asn Leu Ala Glu Ser Ile Glu Val
330 335ttttaaactc gca 1134<210>288<211>337<212>PRT<213>谷氨酸棒杆菌<400>288Met Ser Val Lys Leu Ala Leu Ile Gly Ala Gly Arg Ile Gly Ser Asn1 5 10 15His Ala Arg Leu Ile Thr Asn His Val Ile Gly Ser Glu Leu Val Ala
20 25 30Val Val Asp Pro Thr Pro Asn Ala Glu Thr Leu Ala Asp Glu Leu Gly
35 40 45Ala Val Ala Phe Ser Asn Pro Asp Asp Val Leu Thr Arg Asp Asp Ile
50 55 60Asp Ala Val Leu Ile Ala Thr Pro Ala Arg Thr His Ala Asp Leu Val65 70 75 80Val Lys Ala Ala Ala Ala Gly Lys His Val Phe Val Glu Lys Pro Met
85 90 95Ala Val Thr Leu Glu Asp Ala Asp Arg Ala Ile Asn Ala Ala Arg Glu
100 105 110Ala Asn Thr Val Leu Gln Val Gly Phe Asn Arg Arg Phe Ala Ala Gly
115 120 125Phe Ala Ala Ala Arg Ala Arg Ile Asp Ala Gly Asp Ile Gly Thr Pro
130 135 140Gln Leu Leu Arg Ser Val Thr Arg Asp Pro Gly Pro Phe Thr Ala Asp145 150 155 160Pro Asn Lys Ile Pro Gln Trp Thr Ile Phe Leu Glu Thr Leu Ile His
165 170 175Asp Phe Asp Ala Leu Cys Tyr Leu Asn Pro Gly Ala Thr Pro Val Glu
180 185 190Val Thr Ala His Ala Asp Cys Leu Val Val Pro Glu Ala Ala Gly Thr
195 200 205Gly Phe Leu Asp Thr Ala Val Val Thr Val Arg Phe Asp Asn Gly Ala
210 215 220Ile Gly Thr Ala Glu Ala Ser Phe Ser Ala Ala Tyr Gly Tyr Asp Val225 230 235 240Arg Gly Glu Val Phe Gly Ser Lys Gly Met Met Thr Ala Gly Asp Ala
245 250 255Arg Ala Thr Asn Met Thr Phe Tyr Gly Ala Glu Gly Ile Ala Ala Ala
260 265 270Thr Ser Arg Ala Asp Thr Asp Leu Leu Ser Asp Ala Tyr Arg Ala Glu
275 280 285Phe Gln Ala Phe Val Asp Ser Ile Arg Thr Asn Thr Pro Ser Lys Val
290 295 300Pro Gly Glu Ala Ala Arg Thr Ala Leu Leu Ile Ala Leu Gly Ala Ile305 310 315 320Arg Ser Val Glu Thr Gly Ala Thr Ile Asn Leu Ala Glu Ser Ile Glu
325 330 335Val<210>289<211>996<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(973)<223>RXN00013<400>289ctgcagaaaa ttcgggacgc atgattgcac atattacccg caccgattgt gattcttaga 60acgccacctt attcagcaca cttggccgac ggcatgcaca atg gaa ggc atg act 115
Met Glu Gly Met Thr
1 5aat cca gag cag aca cat ccc gct gca agc ctc gaa gac atg atc aaa 163Asn Pro Glu Gln Thr His Pro Ala Ala Ser Leu Glu Asp Met Ile Lys
10 15 20acc atc aca aag acc ttc gtg att gct cac gat cag gat tct gat gag 211Thr Ile Thr Lys Thr Phe Val Ile Ala His Asp Gln Asp Ser Asp Glu
25 30 35cat ctt gcg cag gca ctg gtg tac aac gct gga cgt ttg gca tgg cgc 259His Leu Ala Gln Ala Leu Val Tyr Asn Ala Gly Arg Leu Ala Trp Arg
40 45 50atg cgc gaa aac ggt gtg gat acg gat tac aag act tct gtg tct gat 307Met Arg Glu Asn Gly Val Asp Thr Asp Tyr Lys Thr Ser Val Ser Asp
55 60 65gtg gtc acg gat gcc gat cgt gcg gcc gag gcc ttc gtc gca ggc gtt 355Val Val Thr Asp Ala Asp Arg Ala Ala Glu Ala Phe Val Ala Gly Val70 75 80 85ctt gaa gcg ttg cgg cct gag gac ggc gtg ctt ggc gag gaa ggc gcg 403Leu Glu Ala Leu Arg Pro Glu Asp G1y Val Leu Gly Glu Glu Gly Ala
90 95 100gac cgg gcg tcg aaa agc gga aaa acc tgg gtc atc gac ccg gtt gat 451Asp Arg Ala Ser Lys Ser Gly Lys Thr Trp Val Ile Asp Pro Val Asp
105 110 115ggc acc tac aac ttc acc cag ggc tca gat tat tgg tgc tcg gcg ctc 499Gly Thr Tyr Asn Phe Thr Gln Gly Ser Asp Tyr Trp Cys Ser Ala Leu
120 125 130gcg ctg gtc gag ggc gat cca tcc gcg cca tcg cgc gtg ctt ttc ggc 547Ala Leu Val Glu Gly Asp Pro Ser Ala Pro Ser Arg Val Leu Phe Gly
135 140 145gcc gta cac cgc cca gcc atg ggt tat acg tgg ttc ggt ggc ccg gga 595Ala Val His Arg Pro Ala Met Gly Tyr Thr Trp Phe Gly Gly Pro Gly150 155 160 165atc cgc acc acg ctc gac ggc aag gag cta gat ttg ctt gtc gac gcc 643Ile Arg Thr Thr Leu Asp Gly Lys Glu Leu Asp Leu Leu Val Asp Ala
170 175 180ccc ctc aat caa atc tcc ctg gcc acc tac atc cac ccg tca cgc atc 691Pro Leu Asn Gln Ile Ser Leu Ala Thr Tyr Ile His Pro Ser Arg Ile
185 190 195gcg gaa cct gat att caa aag gcg tgg atg agc gtt gcc acc cac cct 739Ala Glu Pro Asp Ile Gln Lys Ala Trp Met Ser Val Ala Thr His Pro
200 205 210gca acg ctg cgc atg ttc ggc gcc ggc tcc atc gat ttg gcc aac atc 787Ala Thr Leu Arg Met Phe Gly Ala Gly Ser Ile Asp Leu Ala Asn Ile
215 220 225gcc gac ggc agc atg ggc gca tgg gtg cag cac agc gtc gca gat tgg 835Ala Asp Gly Ser Met Gly Ala Trp Val Gln His Ser Val Ala Asp Trp230 235 240 245gac tgg cta ccc ggc cgc gca ctc atc gaa ggc gtc ggc gga gca tgc 883Asp Trp Leu Pro Gly Arg Ala Leu Ile Glu Gly Val Gly Gly Ala Cys
250 255 260atc aaa gtg acc gcc ggc ggc gtc gaa tgg tcc gtt gca gga aac gcg 931Ile Lys Val Thr Ala Gly Gly Val Glu Trp Ser Val Ala Gly Asn Ala
265 270 275gaa gca gtt agt gag atc tcc gaa act tta agc gca cta gac 973Glu Ala Val Ser Glu Ile Ser Glu Thr Leu Ser Ala Leu Asp
280 285 290taacaacaca tgagcaaata tgc 996<210>290<211>291<212>PRT<213>谷氨酸棒杆菌<400>290Met Glu Gly Met Thr Asn Pro Glu Gln Thr His Pro Ala Ala Ser Leu1 5 10 15Glu Asp Met Ile Lys Thr Ile Thr Lys Thr Phe Val Ile Ala His Asp
20 25 30Gln Asp Ser Asp Glu His Leu Ala Gln Ala Leu Val Tyr Asn Ala Gly
35 40 45Arg Leu Ala Trp Arg Met Arg Glu Asn Gly Val Asp Thr Asp Tyr Lys
50 55 60Thr Ser Val Ser Asp Val Val Thr Asp Ala Asp Arg Ala Ala Glu Ala65 70 75 80Phe Val Ala Gly Val Leu Glu Ala Leu Arg Pro Glu Asp Gly Val Leu
85 90 95Gly Glu Glu Gly Ala Asp Arg Ala Ser Lys Ser Gly Lys Thr Trp Val
100 105 110Ile Asp Pro Val Asp Gly Thr Tyr Asn Phe Thr Gln Gly Ser Asp Tyr
115 120 125Trp Cys Ser Ala Leu Ala Leu Val Glu Gly Asp Pro Ser Ala Pro Ser
130 135 140Arg Val Leu Phe Gly Ala Val His Arg Pro Ala Met Gly Tyr Thr Trp145 150 155 160Phe Gly Gly Pro Gly Ile Arg Thr Thr Leu Asp Gly Lys Glu Leu Asp
165 170 175Leu Leu Val Asp Ala Pro Leu Asn Gln Ile Ser Leu Ala Thr Tyr Ile
180 185 190His Pro Ser Arg Ile Ala Glu Pro Asp Ile Gln Lys Ala Trp Met Ser
195 200 205Val Ala Thr His Pro Ala Thr Leu Arg Met Phe Gly Ala Gly Ser Ile
210 215 220Asp Leu Ala Asn Ile Ala Asp Gly Ser Met Gly Ala Trp Val Gln His225 230 235 240Ser Val Ala Asp Trp Asp Trp Leu Pro Gly Arg Ala Leu Ile Glu Gly
245 250 255Val Gly Gly Ala Cys Ile Lys Val Thr Ala Gly Gly Val Glu Trp Ser
260 265 270Val Ala Gly Asn Ala Glu Ala Val Ser Glu Ile Ser Glu Thr Leu Ser
275 280 285Ala Leu Asp
290<210>291<211>996<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(973)<223>FRXA00013<400>291ctgcagaaaa ttcgggacgc atgattgcac atattacccg caccgattgt gattcttaga 60acgccacctt attcagcaca cttggccgac ggcatgcaca atg gaa ggc atg act 115
Met Glu Gly Met Thr
1 5aat cca gag cag aca cat ccc gct gca agc ctc gaa gac atg atc aaa 163Asn Pro Glu Gln Thr His Pro Ala Ala Ser Leu Glu Asp Met Ile Lys
10 15 20acc atc aca aag acc ttc gtg att gct cac gat cag gat tct gat gag 211Thr Ile Thr Lys Thr Phe Val Ile Ala His Asp Gln Asp Ser Asp Glu
25 30 35cat ctt gcg cag gca ctg gtg tac aac gct gga cgt ttg gca tgg cgc 259His Leu Ala Gln Ala Leu Val Tyr Asn Ala Gly Arg Leu Ala Trp Arg
40 45 50atg cgc gaa aac ggt gtg gat acg gat tac aag act tct gtg tct gat 307Met Arg Glu Asn Gly Val Asp Thr Asp Tyr Lys Thr Ser Val Ser Asp
55 60 65gtg gtc acg gat gcc gat cgt gcg gcc gag gcc ttc gtc gca ggc gtt 355Val Val Thr Asp Ala Asp Arg Ala Ala Glu Ala Phe Val Ala Gly Val70 75 80 85ctt gaa gcg ttg cgg cct gag gac ggc gtg ctt ggc gag gaa ggc gcg 403Leu Glu Ala Leu Arg Pro Glu Asp Gly Val Leu Gly Glu Glu Gly Ala
90 95 100gac cgg gcg tcg aaa agc gga aaa acc tgg gtc atc gac ccg gtt gat 451Asp Arg Ala Ser Lys Ser Gly Lys Thr Trp Val Ile Asp Pro Val Asp
105 110 115ggc acc tac aac ttc acc cag ggc tca gat tat tgg tgc tcg gcg ctc 499Gly Thr Tyr Asn Phe Thr Gln Gly Ser Asp Tyr Trp Cys Ser Ala Leu
120 125 130gcg ctg gtc gag ggc gat cca tcc gcg cca tcg cgc gtg ctt ttc ggc 547Ala Leu Val Glu Gly Asp Pro Ser Ala Pro Ser Arg Val Leu Phe Gly
135 140 145gcc gta cac cgc cca gcc atg ggt tat acg tgg ttc ggt ggc ccg gga 595Ala Val His Arg Pro Ala Met Gly Tyr Thr Trp Phe Gly Gly Pro Gly150 155 160 165atc cgc acc acg ctc gac ggc aag gag cta gat ttg ctt gtc gac gcc 643Ile Arg Thr Thr Leu Asp Gly Lys Glu Leu Asp Leu Leu Val Asp Ala
170 175 180ccc ctc aat caa atc tcc ctg gcc acc tac atc cac ccg tca cgc atc 691Pro Leu Asn Gln Ile Ser Leu Ala Thr Tyr Ile His Pro Ser Arg Ile
185 190 195gcg gaa cct gat att caa aag gcg tgg atg agc gtt gcc acc cac cct 739Ala Glu Pro Asp Ile Gln Lys Ala Trp Met Ser Val Ala Thr His Pro
200 205 210gca acg ctg cgc atg ttc ggc gcc ggc tcc atc gat ttg gcc aac atc 787Ala Thr Leu Arg Met Phe Gly Ala Gly Ser Ile Asp Leu Ala Asn Ile
215 220 225gcc gac ggc agc atg ggc gca tgg gtg cag cac agc gtc gca gat tgg 835Ala Asp Gly Ser Met Gly Ala Trp Val Gln His Ser Val Ala Asp Trp230 235 240 245gac tgg cta ccc ggc cgc gca ctc atc gaa ggc gtc ggc gga gcg tgc 883Asp Trp Leu Pro Gly Arg Ala Leu Ile Glu Gly Val Gly Gly Ala Cys
250 255 260atc aaa gtg acc gcc ggc ggc gtc gaa tgg tcc gtt gca gga aac gcg 931Ile Lys Val Thr Ala Gly Gly Val Glu Trp Ser Val Ala Gly Asn Ala
265 270 275gaa gca gtt agt gag atc tcc gaa act tta agc gca cta gac 973Glu Ala Val Ser Glu Ile Ser Glu Thr Leu Ser Ala Leu Asp
280 285 290tagcaacaca tgagcaaata tgc 996<210>292<211>291<212>PRT<213>谷氨酸棒杆菌<400>292Met Glu Gly Met Thr Asn Pro Glu Gln Thr His Pro Ala Ala Ser Leu1 5 10 15Glu Asp Met Ile Lys Thr Ile Thr Lys Thr Phe Val Ile Ala His Asp
20 25 30Gln Asp Ser Asp Glu His Leu Ala Gln Ala Leu Val Tyr Asn Ala Gly
35 40 45Arg Leu Ala Trp Arg Met Arg Glu Asn Gly Val Asp Thr Asp Tyr Lys
50 55 60Thr Ser Val Ser Asp Val Val Thr Asp Ala Asp Arg Ala Ala Glu Ala65 70 75 80Phe Val Ala Gly Val Leu Glu Ala Leu Arg Pro Glu Asp Gly Val Leu
85 90 95Gly Glu Glu Gly Ala Asp Arg Ala Ser Lys Ser Gly Lys Thr Trp Val
100 105 110Ile Asp Pro Val Asp Gly Thr Tyr Asn Phe Thr Gln Gly Ser Asp Tyr
115 120 125Trp Cys Ser Ala Leu Ala Leu Val Glu Gly Asp Pro Ser Ala Pro Ser
130 135 140Arg Val Leu Phe Gly Ala Val His Arg Pro Ala Met Gly Tyr Thr Trp145 150 155 160Phe Gly Gly Pro Gly Ile Arg Thr Thr Leu Asp Gly Lys Glu Leu Asp
165 170 175Leu Leu Val Asp Ala Pro Leu Asn Gln Ile Ser Leu Ala Thr Tyr Ile
180 185 190His Pro Ser Arg Ile Ala Glu Pro Asp Ile Gln Lys Ala Trp Met Ser
195 200 205Val Ala Thr His Pro Ala Thr Leu Arg Met Phe Gly Ala Gly Ser Ile
210 215 220Asp Leu Ala Asn Ile Ala Asp Gly Ser Met Gly Ala Trp Val Gln His225 230 235 240Ser Val Ala Asp Trp Asp Trp Leu Pro Gly Arg Ala Leu Ile Glu Gly
245 250 255Val Gly Gly Ala Cys Ile Lys Val Thr Ala Gly Gly Val Glu Trp Ser
260 265 270Val Ala Gly Asn Ala Glu Ala Val Ser Glu Ile Ser Glu Thr Leu Ser
275 280 285Ala Leu Asp
290<210>293<211>948<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(925)<223>RXA01099<400>293ggatgagggc attgattccg tcatcattgg caaggcactt tatgagcaca agttcaccct 60cgaagaggct ttggctgcag tagaaaagct cggttaatac atg gat gct cgt ggg 115
Met Asp Ala Arg Gly
1 5atg ttg gcc att gcg gag gcc gtt gta gat gat gcc gaa gcc ctc ttc 163Met Leu Ala Ile Ala Glu Ala Val Val Asp Asp Ala Glu Ala Leu Phe
10 15 20atg cag ggc ttc gga gct gca cct gcc cat atg aaa tcc ccg ggg gat 211Met Gln Gly Phe Gly Ala Ala Pro Ala His Met Lys Ser Pro Gly Asp
25 30 35ttt gcc acg gaa gtg gat atg gcc atc gaa tcc cat atg cgt tcg atg 259Phe Ala Thr Glu Val Asp Met Ala Ile Glu Ser His Met Arg Ser Met
40 45 50ctg aac atg atg aca ggc att gct gtc atc ggt gaa gaa ggt ggc ggt 307Leu Asn Met Met Thr Gly Ile Ala Val Ile Gly Glu Glu Gly Gly Gly
55 60 65gcg acc tcc ggc acg cgc tgg gtg att gat ccc atc gac ggc acc gcc 355Ala Thr Ser Gly Thr Arg Trp Val Ile Asp Pro Ile Asp Gly Thr Ala70 75 80 85aac ttc gcg gcg tcc aac ccg atg agc gcg atc ctg gtg tct ttg ctt 403Asn Phe Ala Ala Ser Asn Pro Met Ser Ala Ile Leu Val Ser Leu Leu
90 95 100gtc gac gac cag ccc gtc ctg ggt att acc tcc atg ccc atg ctg ggt 451Val Asp Asp Gln Pro Val Leu Gly Ile Thr Ser Met Pro Met Leu Gly
105 110 115aaa cgc ctc acc gct ttt gaa ggt tca ccg ctg atg atc aac ggt gaa 499Lys Arg Leu Thr Ala Phe Glu Gly Ser Pro Leu Met Ile Asn Gly Glu
120 125 130cct cag gaa cca ttg caa gaa caa tcc agt ttg gta tcc cac att ggt 547Pro Gln Glu Pro Leu Gln Glu Gln Ser Ser Leu Val Ser His Ile Gly
135 140 145ttt agt tcc atg gcc tcc ccg cgc aat aca gcg ttt cct gtg gag ttg 595Phe Ser Ser Met Ala Ser Pro Arg Asn Thr Ala Phe Pro Val Glu Leu150 155 160 165cgt cgg gat ctt ctg acc gag ctc acg gaa tcg tat ctt cgt ccc cgc 643Arg Arg Asp Leu Leu Thr Glu Leu Thr Glu Ser Tyr Leu Arg Pro Arg
170 175 180att aca ggt tcg gtg ggt gtt gat ctc gcg ttc act gcg cag ggc att 691Ile Thr Gly Ser Val Gly Val Asp Leu Ala Phe Thr Ala Gln Gly Ile
185 190 195ttt gga gca tgc gta tcg ttt agt cct cat gtt tgg gac aat tcc gca 739Phe Gly Ala Cys Val Ser Phe Ser Pro His Val Trp Asp Asn Ser Ala
200 205 210ggc gtg atg ttg atg cgc gct gct ggt gca caa gtt act gac acc gaa 787Gly Val Met Leu Met Arg Ala Ala Gly Ala Gln Val Thr Asp Thr Glu
215 220 225ggc cat ccg tgg gca cca ggt agg gga gtc gtg gcc gga aca aaa agg 835Gly His Pro Trp Ala Pro Gly Arg Gly Val Val Ala Gly Thr Lys Arg230 235 240 245gct cac gat gtg ctg tta agt aag att gaa aaa gtt cgg ttg atg cat 883Ala His Asp Val Leu Leu Ser Lys Ile Glu Lys Val Arg Leu Met His
250 255 260gca gat gca ggt aat gac cag tcg tta aat gag gag tac aag 925Ala Asp Ala Gly Asn Asp Gln Ser Leu Asn Glu Glu Tyr Lys
265 270 275taaaatgggc gtggcaattc gag 948<210>294<211>275<212>PRT<213>谷氨酸棒杆菌<400>294Met Asp Ala Arg Gly Met Leu Ala Ile Ala Glu Ala Val Val Asp Asp1 5 10 15Ala Glu Ala Leu Phe Met Gln Gly Phe Gly Ala Ala Pro Ala His Met
20 25 30Lys Ser Pro Gly Asp Phe Ala Thr Glu Val Asp Met Ala Ile Glu Ser
35 40 45His Met Arg Ser Met Leu Asn Met Met Thr Gly Ile Ala Val Ile Gly
50 55 60Glu Glu Gly Gly Gly Ala Thr Ser Gly Thr Arg Trp Val Ile Asp Pro65 70 75 80Ile Asp Gly Thr Ala Asn Phe Ala Ala Ser Asn Pro Met Ser Ala Ile
85 90 95Leu Val Ser Leu Leu Val Asp Asp Gln Pro Val Leu Gly Ile Thr Ser
100 105 110Met Pro Met Leu Gly Lys Arg Leu Thr Ala Phe Glu Gly Ser Pro Leu
115 120 125Met Ile Asn Gly Glu Pro Gln Glu Pro Leu Gln Glu Gln Ser Ser Leu
130 135 140Val Ser His Ile Gly Phe Ser Ser Met Ala Ser Pro Arg Asn Thr Ala145 150 155 160Phe Pro Val Glu Leu Arg Arg Asp Leu Leu Thr Glu Leu Thr Glu Ser
165 170 175Tyr Leu Arg Pro Arg Ile Thr Gly Ser Val Gly Val Asp Leu Ala Phe
180 185 190Thr Ala Gln Gly Ile Phe Gly Ala Cys Val Ser Phe Ser Pro His Val
195 200 205Trp Asp Asn Ser Ala Gly Val Met Leu Met Arg Ala Ala Gly Ala Gln
210 215 220Val Thr Asp Thr Glu Gly His Pro Trp Ala Pro Gly Arg Gly Val Val225 230 235 240Ala Gly Thr Lys Arg Ala His Asp Val Leu Leu Ser Lys Ile Glu Lys
245 250 255Val Arg Leu Met His Ala Asp Ala Gly Asn Asp Gln Ser Leu Asn Glu
260 265 270Glu Tyr Lys
275<210>295<211>576<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(576)<223>RXN01332<400>295cac atc tct gcc att att gag ccc gac gca gca cgt gcc gct gca gct 48His Ile Ser Ala Ile Ile Glu Pro Asp Ala Ala Arg Ala Ala Ala Ala1 5 10 15gca gaa gac gcg ccg ggt gca cag gcc ttc act cgc att gaa gat gct 96Ala Glu Asp Ala Pro Gly Ala Gln Ala Phe Thr Arg Ile Glu Asp Ala
20 25 30atc gca gcc gat gct gtc gac gca gtg ctg atc gcc gta cca ggt cag 144Ile Ala Ala Asp Ala Val Asp Ala Val Leu Ile Ala Val Pro Gly Gln
35 40 45ttc cat gag cca gta ctt gtc cca gca cta gaa gca ggc ctt ccc atc 192Phe His Glu Pro Val Leu Val Pro Ala Leu Glu Ala Gly Leu Pro Ile
50 55 60ctg tgt gaa aag cca ctg acc cca gat tct gaa tcc tca ctg cgc atc 240Leu Cys Glu Lys Pro Leu Thr Pro Asp Ser Glu Ser Ser Leu Arg Ile65 70 75 80gtc gag ctg gag cag aag ctg gac aag cca cac atc cag gtt ggt ttc 288Val Glu Leu Glu Gln Lys Leu Asp Lys Pro His Ile Gln Val Gly Phe
85 90 95atg cgc cgc ttc gac cct gag tac aac aac ttg cgc aaa ttg gtg gaa 336Met Arg Arg Phe Asp Pro Glu Tyr Asn Asn Leu Arg Lys Leu Val Glu
100 105 110tcc ggc gaa gct ggc gaa ctg ctc atg ctc cgc ggc ctg cac cgc aac 384Ser Gly Glu Ala Gly Glu Leu Leu Met Leu Arg Gly Leu His Arg Asn
115 120 125cca agt gtt ggt gag agc tac acc cag tcc atg ctg atc acc gac tcc 432Pro Ser Val Gly Glu Ser Tyr Thr Gln Ser Met Leu Ile Thr Asp Ser
130 135 140gtc gtc cac gaa ttc gat gtc atc cca tgg ctc gca ggc tcc cga gtt 480Val Val His Glu Phe Asp Val Ile Pro Trp Leu Ala Gly Ser Arg Val145 150 155 160gtc tcc gtt gaa gtg aag tac cca aag acc tcc tca ctg gcg cac tcc 528Val Ser Val Glu Val Lys Tyr Pro Lys Thr Ser Ser Leu Ala His Ser
165 170 175ggc ctc aag gaa cca atc ctg gtg atc atg gag ctc gaa aac ggc gtg 576Gly Leu Lys Glu Pro Ile Leu Val Ile Met Glu Leu Glu Asn Gly Val
180 185 190<210>296<211>192<212>PRT<213>谷氨酸棒杆菌<400>296His Ile Ser Ala Ile Ile Glu Pro Asp Ala Ala Arg Ala Ala Ala Ala1 5 10 15Ala Glu Asp Ala Pro Gly Ala Gln Ala Phe Thr Arg Ile Glu Asp Ala
20 25 30Ile Ala Ala Asp Ala Val Asp Ala Val Leu Ile Ala Val Pro Gly Gln
35 40 45Phe His Glu Pro Val Leu Val Pro Ala Leu Glu Ala Gly Leu Pro Ile
50 55 60Leu Cys Glu Lys Pro Leu Thr Pro Asp Ser Glu Ser Ser Leu Arg Ile65 70 75 80Val Glu Leu Glu Gln Lys Leu Asp Lys Pro His Ile Gln Val Gly Phe
85 90 95Met Arg Arg Phe Asp Pro Glu Tyr Asn Asn Leu Arg Lys Leu Val Glu
100 105 110Ser Gly Glu Ala Gly Glu Leu Leu Met Leu Arg Gly Leu His Arg Asn
115 120 125Pro Ser Val Gly Glu Ser Tyr Thr Gln Ser Met Leu Ile Thr Asp Ser
130 135 140Val Val His Glu Phe Asp Val Ile Pro Trp Leu Ala Gly Ser Arg Val145 150 155 160Val Ser Val Glu Val Lys Tyr Pro Lys Thr Ser Ser Leu Ala His Ser
165 170 175Gly Leu Lys Glu Pro Ile Leu Val Ile Met Glu Leu Glu Asn Gly Val
180 185 190<210>297<211>549<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(549)<223>FRXA01332<400>297gca gca cgt gcc gct gca gct gca gaa gac gcg ccg ggt gca cag gcc 48Ala Ala Arg Ala Ala Ala Ala Ala Glu Asp Ala Pro Gly Ala Gln Ala1 5 10 15ttc act cgc att gaa gat gct atc gca gcc gat gct gtc gac gca gtg 96Phe Thr Arg Ile Glu Asp Ala Ile Ala Ala Asp Ala Val Asp Ala Val
20 25 30ctg atc gcc gta cca ggt cag ttc cat gag cca gta ctt gtc cca gca 144Leu Ile Ala Val Pro Gly Gln Phe His Glu Pro Val Leu Val Pro Ala
35 40 45cta gaa gca ggc ctt ccc atc ctg tgt gaa aag cca ctg acc cca gat 192Leu Glu Ala Gly Leu Pro Ile Leu Cys Glu Lys Pro Leu Thr Pro Asp
50 55 60tct gaa tcc tca ctg cgc atc gtc gag ctg gag cag aag ctg gac aag 240Ser Glu Ser Ser Leu Arg Ile Val Glu Leu Glu Gln Lys Leu Asp Lys65 70 75 80cca cac atc cag gtt ggt ttc atg cgc cgc ttc gac cct gag tac aac 288Pro His Ile Gln Val Gly Phe Met Arg Arg Phe Asp Pro Glu Tyr Asn
85 90 95aac ttg cgc aaa ttg gtg gaa tcc ggc gaa gct ggc gaa ctg ctc atg 336Asn Leu Arg Lys Leu Val Glu Ser Gly Glu Ala Gly Glu Leu Leu Met
100 105 110ctc cgc ggc ctg cac cgc aac cca agt gtt ggt gag agc tac acc cag 384Leu Arg Gly Leu His Arg Asn Pro Ser Val Gly Glu Ser Tyr Thr Gln
115 120 125tcc atg ctg atc acc gac tcc gtc gtc cac gaa ttc gat gtc atc cca 432Ser Met Leu Ile Thr Asp Ser Val Val His Glu Phe Asp Val Ile Pro
130 135 140tgg ctc gca ggc tcc cga gtt gtc tcc gtt gaa gtg aag tac cca aag 480Trp Leu Ala Gly Ser Arg Val Val Ser Val Glu Val Lys Tyr Pro Lys145 150 155 160acc tcc tca ctg gcg cac tcc ggc ctc aag gaa cca atc ctg gtg atc 528Thr Ser Ser Leu Ala His Ser Gly Leu Lys Glu Pro Ile Leu Val Ile
165 170 175atg gag ctc gaa aac ggc gtg 549Met Glu Leu Glu Asn Gly Val
180<210>298<211>183<212>PRT<213>谷氨酸棒杆菌<400>298Ala Ala Arg Ala Ala Ala Ala Ala Glu Asp Ala Pro Gly Ala Gln Ala1 5 10 15Phe Thr Arg Ile Glu Asp Ala Ile Ala Ala Asp Ala Val Asp Ala Val
20 25 30Leu Ile Ala Val Pro Gly Gln Phe His Glu Pro Val Leu Val Pro Ala
35 40 45Leu Glu Ala Gly Leu Pro Ile Leu Cys Glu Lys Pro Leu Thr Pro Asp
50 55 60Ser Glu Ser Ser Leu Arg Ile Val Glu Leu Glu Gln Lys Leu Asp Lys65 70 75 80Pro His Ile Gln Val Gly Phe Met Arg Arg Phe Asp Pro Glu Tyr Asn
85 90 95Asn Leu Arg Lys Leu Val Glu Ser Gly Glu Ala Gly Glu Leu Leu Met
100 105 110Leu Arg Gly Leu His Arg Asn Pro Ser Val Gly Glu Ser Tyr Thr Gln
115 120 125Ser Met Leu Ile Thr Asp Ser Val Val His Glu Phe Asp Val Ile Pro
130 135 140Trp Leu Ala Gly Ser Arg Val Val Ser Val Glu Val Lys Tyr Pro Lys145 150 155 160Thr Ser Ser Leu Ala His Ser Gly Leu Lys Glu Pro Ile Leu Val Ile
165 170 175Met Glu Leu Glu Asn Gly Val
180<210>299<211>1128<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1105)<223>RXA01632<400>299aagggctgca acgtgctttc gacaccacca tcgcagcgtt tgaacaagct gctcgtctcg 60ccccctccac taactgatct ttgaaaggct gaaaaaactc atg act crt cgt atc 115
Met Thr Leu Arg Ile
1 5gcc ctt ttc ggc gct ggc cgc atc ggt cac gtc cac gct gcc aac att 163Ala Leu Phe Gly Ala Gly Arg Ile Gly His Val His Ala Ala Asn Ile
10 15 20gct gca aac cct gat ctt gaa ctc gtt gtt atc gcc gat cct ttc att 211Ala Ala Asn Pro Asp Leu Glu Leu Val Val Ile Ala Asp Pro Phe Ile
25 30 35gaa ggc gca cag cgt ttg gca gaa gcc aat ggg gca gaa gcg gtt gca 259Glu Gly Ala Gln Arg Leu Ala Glu Ala Asn Gly Ala Glu Ala Val Ala
40 45 50tca cca gat gag gtg ttc gcc cgc gat gat atc gat ggc atc gtg atc 307Ser Pro Asp Glu Val Phe Ala Arg Asp Asp Ile Asp Gly Ile Val Ile
55 60 65ggt tca cca acc agc acc cac gtt gat ctg atc acc cgc gcc gtg gaa 355Gly Ser Pro Thr Ser Thr His Val Asp Leu Ile Thr Arg Ala Val Glu70 75 80 85cgt ggc att cct gca ctg tgc gaa aaa ccc att gat tta gac att gaa 403Arg Gly Ile Pro Ala Leu Cys Glu Lys Pro Ile Asp Leu Asp Ile Glu
90 95 100atg gtg cgt gcc tgc aaa gag aag atc ggc gac ggc gct tcc aag gtg 451Met Val Arg Ala Cys Lys Glu Lys Ile Gly Asp Gly Ala Ser Lys Val
105 110 115atg ctg ggg ttt aac cga cgc ttc gat cct tct ttc gct gcc atc aat 499Met Leu Gly Phe Asn Arg Arg Phe Asp Pro Ser Phe Ala Ala Ile Asn
120 125 130gcg cga gtg gca aac cag gag atc ggc aac ctg gag cag ttg gtg atc 547Ala Arg Val Ala Asn Gln Glu Ile Gly Asn Leu Glu Gln Leu Val Ile
135 140 145atc agc cgc gat cca gcg ccc gca ccg aag gac tac atc gca ggt tcc 595Ile Ser Arg Asp Pro Ala Pro Ala Pro Lys Asp Tyr Ile Ala Gly Ser150 155 160 165ggt gga atc ttc cgc gat atg acc atc cac gat ctg gat atg gcg cgt 643Gly Gly Ile Phe Arg Asp Met Thr Ile His Asp Leu Asp Met Ala Arg
170 175 180ttc ttt gtg ccc aat atc gtg gaa gtg act gca acc ggc gcc aat gtt 691Phe Phe Val Pro Asn Ile Val Glu Val Thr Ala Thr Gly Ala Asn Val
185 190 195ttc agc cag gaa atc gcg gag ttc aat gac tac gac cag gtt atc gtc 739Phe Ser Gln Glu Ile Ala Glu Phe Asn Asp Tyr Asp Gln Val Ile Val
200 205 210acg ctt cgt ggc tca aag ggc gag ttg atc aac atc gtg aac tcc cgc 787Thr Leu Arg Gly Ser Lys Gly Glu Leu Ile Asn Ile Val Asn Ser Arg
215 220 225cac tgc tcc tac ggc tac gac cag cga ctt gag gct ttc ggc tct aag 835His Cys Ser Tyr Gly Tyr Asp Gln Arg Leu Glu Ala Phe Gly Ser Lys230 235 240 245ggc atg ctc gcc gcc gac aac atc agg ccc acc acg gtg cgc aag cac 883Gly Met Leu Ala Ala Asp Asn Ile Arg Pro Thr Thr Val Arg Lys His
250 255 260aat gcg gaa agc acc gag cag gca gat ccg att ttc aac ttc ttc ctc 931Asn Ala Glu Ser Thr Glu Gln Ala Asp Pro Ile Phe Asn Phe Phe Leu
265 270 275gag cgc tac gac gcc gct tac aag gca gag ctc gca act ttt gct caa 979Glu Arg Tyr Asp Ala Ala Tyr Lys Ala Glu Leu Ala Thr Phe Ala Gln
280 285 290gga atc cgc gac ggc caa ggc ttc tca cca aac ttc gag gac ggc gtc 1027Gly Ile Arg Asp Gly Gln Gly Phe Ser Pro Asn Phe Glu Asp Gly Val
295 300 305atc gcc ctt gaa cta gcg aat gca tgc ctt gaa tca gct caa acc ggc 1075Ile Ala Leu Glu Leu Ala Asn Ala Cys Leu Glu Ser Ala Gln Thr Gly310 315 320 325cgc acc gtc acc ctc aac cct gcc aac gtt tagtcaacgt ctagttaatg 1125Arg Thr Val Thr Leu Asn Pro Ala Asn Val
330 335cct 1128<210>300<211>335<212>PRT<213>谷氨酸棒杆菌<400>300Met Thr Leu Arg Ile Ala Leu Phe Gly Ala Gly Arg Ile Gly His Val1 5 10 15His Ala Ala Asn Ile Ala Ala Asn Pro Asp Leu Glu Leu Val Val Ile
20 25 30Ala Asp Pro Phe Ile Glu Gly Ala Gln Arg Leu Ala Glu Ala Asn Gly
35 40 45Ala Glu Ala Val Ala Ser Pro Asp Glu Val Phe Ala Arg Asp Asp Ile
50 55 60Asp Gly Ile Val Ile Gly Ser Pro Thr Ser Thr His Val Asp Leu Ile65 70 75 80Thr Arg Ala Val Glu Arg Gly Ile Pro Ala Leu Cys Glu Lys Pro Ile
85 90 95Asp Leu Asp Ile Glu Met Val Arg Ala Cys Lys Glu Lys Ile Gly Asp
100 105 110Gly Ala Ser Lys Val Met Leu Gly Phe Asn Arg Arg Phe Asp Pro Ser
115 120 125Phe Ala Ala Ile Asn Ala Arg Val Ala Asn Gln Glu Ile Gly Asn Leu
130 135 140Glu Gln Leu Val Ile Ile Ser Arg Asp Pro Ala Pro Ala Pro Lys Asp145 150 155 160Tyr Ile Ala Gly Ser Gly Gly Ile Phe Arg Asp Met Thr Ile His Asp
165 170 175Leu Asp Met Ala Arg Phe Phe Val Pro Asn Ile Val Glu Val Thr Ala
180 185 190Thr Gly Ala Asn Val Phe Ser Gln Glu Ile Ala Glu Phe Asn Asp Tyr
195 200 205Asp Gln Val Ile Val Thr Leu Arg Gly Ser Lys Gly Glu Leu Ile Asn
210 215 220Ile Val Asn Ser Arg His Cys Ser Tyr Gly Tyr Asp Gln Arg Leu Glu225 230 235 240Ala Phe Gly Ser Lys Gly Met Leu Ala Ala Asp Asn Ile Arg Pro Thr
245 250 255Thr Val Arg Lys His Asn Ala Glu Ser Thr Glu Gln Ala Asp Pro Ile
260 265 270Phe Asn Phe Phe Leu Glu Arg Tyr Asp Ala Ala Tyr Lys Ala Glu Leu
275 280 285Ala Thr Phe Ala Gln Gly Ile Arg Asp Gly Gln Gly Phe Ser Pro Asn
290 295 300Phe Glu Asp Gly Val Ile Ala Leu Glu Leu Ala Asn Ala Cys Leu Glu305 310 315 320Ser Ala Gln Thr Gly Arg Thr Val Thr Leu Asn Pro Ala Asn Val
325 330 335<210>301<211>1206<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1183)<223>RXA01633<400>301gcgaatgcat gccttgaatc agctcaaacc ggccgcaccg tcaccctcaa ccctgccaac 60gtttagtcaa cgtctagtta atgcctaagg agaaaacctc atg aaa aac atc acc 115
Met Lys Asn Ile Thr
1 5atc gga atg gtc ggc gtc ggc cgc att ggc cgc atg cac gtc gcc aac 163Ile Gly Met Val Gly Val Gly Arg Ile Gly Arg Met His Val Ala Asn
10 15 20atg ctt gcc gtt gct gaa act ttg aag gaa cgc gac ctc aac att gag 211Met Leu Ala Val Ala Glu Thr Leu Lys Glu Arg Asp Leu Asn Ile Glu
25 30 35atc gtg ctc gca gac gca atg ccc ggt ttt gcg gag cag gtg ggc gcg 259Ile Val Leu Ala Asp Ala Met Pro Gly Phe Ala Glu Gln Val Gly Ala
40 45 50gac atg ggc gtg aag gcg gcg gca agc gtc gat aag ctt att gag gac 307Asp Met Gly Val Lys Ala Ala Ala Ser Val Asp Lys Leu Ile Glu Asp
55 60 65ggg gtg gat gcc ctt ttc att gcc acc agc acc gct ggc cac gtc gat 355Gly Val Asp Ala Leu Phe Ile Ala Thr Ser Thr Ala Gly His Val Asp70 75 80 85gtt ttg cgc aag ggc atc gcg gca aag ctg ccg atg ttc tgc gag aag 403Val Leu Arg Lys Gly Ile Ala Ala Lys Leu Pro Met Phe Cys Glu Lys
90 95 100ccg atc gcg tcg gat gtg cct gag tcg ctg aac atc atc cgc gaa att 451Pro Ile Ala Ser Asp Val Pro Glu Ser Leu Asn Ile Ile Arg Glu Ile
105 110 115gat gcg gct ggc gcg acg gtt cag gtc ggc cac cag cgc cgt ttt gac 499Asp Ala Ala Gly Ala Thr Val Gln Val Gly His Gln Arg Arg Phe Asp
120 125 130ctc ggt tac cag gaa gct aaa cga cgc cta gat gca ggc gac ctc ggc 547Leu Gly Tyr Gln Glu Ala Lys Arg Arg Leu Asp Ala Gly Asp Leu Gly
135 140 145tgg ctt cat tcg ctc aag gcc gta tcg agc gat gcg ttt ccg cca ccg 595Trp Leu His Ser Leu Lys Ala Val Ser Ser Asp Ala Phe Pro Pro Pro150 155 160 165gtg tcc tac tgc gct acc tct ggt gga ctt ttc cgc gat gtg tcg ctg 643Val Ser Tyr Cys Ala Thr Ser Gly Gly Leu Phe Arg Asp Val Ser Leu
170 175 180cac gat ttc gac atc att cgc tgg ctg acc ggc cag gat att gtc gag 691His Asp Phe Asp Ile Ile Arg Trp Leu Thr Gly Gln Asp Ile Val Glu
185 190 195gtg tac gcc aag ggc agc aac aac ggc gac cca gaa atc ggc gca gtc 739Val Tyr Ala Lys Gly Ser Asn Asn Gly Asp Pro Glu Ile Gly Ala Val
200 205 210ggt gac atc gat acc gga gcg gcc cta ctc acg ctt gcc gac ggc acc 787Gly Asp Ile Asp Thr Gly Ala Ala Leu Leu Thr Leu Ala Asp Gly Thr
215 220 225ctc gcc acc gcc atc gcc act cgt tac aac ggt gca ggc cac gac gtt 835Leu Ala Thr Ala Ile Ala Thr Arg Tyr Asn Gly Ala Gly His Asp Val230 235 240 245cgc ctc gat gtt atg ggc tct aaa gat tcc acg atc gtt ggc ctg gat 883Arg Leu Asp Val Met Gly Ser Lys Asp Ser Thr Ile Val Gly Leu Asp
250 255 260gaa aag tct gcg ttc gct tct gcg gag gag ggc atc gat ttc cca acc 931Glu Lys Ser Ala Phe Ala Ser Ala Glu Glu Gly Ile Asp Phe Pro Thr
265 270 275ggc gaa tcg cac cca acg ttt gcc gag cgc ttc gcc gac gca tac aag 979Gly Glu Ser His Pro Thr Phe Ala Glu Arg Phe Ala Asp Ala Tyr Lys
280 285 290aat gag tgc att gcg ttc gtg gag ttg atc ctg gga gag cgg gaa aac 1027Asn Glu Cys Ile Ala Phe Val Glu Leu Ile Leu Gly Glu Arg Glu Asn
295 300 305cct tgt acc cct gca gac gct gtg gct gcg gcg att gtt gcc gat gca 1075Pro Cys Thr Pro Ala Asp Ala Val Ala Ala Ala Ile Val Ala Asp Ala310 315 320 325gct cag ctg tcg ctg gtc act ggc gag cca gtg aag att cct act gta 1123Ala Gln Leu Ser Leu Val Thr Gly Glu Pro Val Lys Ile Pro Thr Val
330 335 340cgg gaa att ctt gaa ggt tct gcg cag cca gtt gag gtg cgt gcg ctg 1171Arg Glu Ile Leu Glu Gly Ser Ala Gln Pro Val Glu Val Arg Ala Leu
345 350 355gtt cca tct gct taaaacctta ctgcttatct aaa 1206Val Pro Ser Ala
360<210>302<211>361<212>PRT<213>谷氨酸棒杆菌<400>302Met Lys Asn Ile Thr Ile Gly Met Val Gly Val Gly Arg Ile Gly Arg1 5 10 15Met His Val Ala Asn Met Leu Ala Val Ala Glu Thr Leu Lys Glu Arg
20 25 30Asp Leu Asn Ile Glu Ile Val Leu Ala Asp Ala Met Pro Gly Phe Ala
35 40 45Glu Gln Val Gly Ala Asp Met Gly Val Lys Ala Ala Ala Ser Val Asp
50 55 60Lys Leu Ile Glu Asp Gly Val Asp Ala Leu Phe Ile Ala Thr Ser Thr65 70 75 80Ala Gly His Val Asp Val Leu Arg Lys Gly Ile Ala Ala Lys Leu Pro
85 90 95Met Phe Cys Glu Lys Pro Ile Ala Ser Asp Val Pro Glu Ser Leu Asn
100 105 110Ile Ile Arg Glu Ile Asp Ala Ala Gly Ala Thr Val Gln Val Gly His
115 120 125Gln Arg Arg Phe Asp Leu Gly Tyr Gln Glu Ala Lys Arg Arg Leu Asp
130 135 140Ala Gly Asp Leu Gly Trp Leu His Ser Leu Lys Ala Val Ser Ser Asp145 150 155 160Ala Phe Pro Pro Pro Val Ser Tyr Cys Ala Thr Ser Gly Gly Leu Phe
165 170 175Arg Asp Val Ser Leu His Asp Phe Asp Ile Ile Arg Trp Leu Thr Gly
180 185 190Gln Asp Ile Val Glu Val Tyr Ala Lys Gly Ser Asn Asn Gly Asp Pro
195 200 205Glu Ile Gly Ala Val Gly Asp Ile Asp Thr Gly Ala Ala Leu Leu Thr
210 215 220Leu Ala Asp Gly Thr Leu Ala Thr Ala Ile Ala Thr Arg Tyr Asn Gly225 230 235 240Ala Gly His Asp Val Arg Leu Asp Val Met Gly Ser Lys Asp Ser Thr
245 250 255Ile Val Gly Leu Asp Glu Lys Ser Ala Phe Ala Ser Ala Glu Glu Gly
260 265 270Ile Asp Phe Pro Thr Gly Glu Ser His Pro Thr Phe Ala Glu Arg Phe
275 280 285Ala Asp Ala Tyr Lys Asn Glu Cys Ile Ala Phe Val Glu Leu Ile Leu
290 295 300Gly Glu Arg Glu Asn Pro Cys Thr Pro Ala Asp Ala Val Ala Ala Ala305 310 315 320Ile Val Ala Asp Ala Ala Gln Leu Ser Leu Val Thr Gly Glu Pro Val
325 330 335Lys Ile Pro Thr Val Arg Glu Ile Leu Glu Gly Ser Ala Gln Pro Val
340 345 350Glu Val Arg Ala Leu Val Pro Ser Ala
355 360<210>303<211>1146<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1123)<223>RXN01406<400>303gttcctcatt cctctaatcg gcgcactatc tttgcctcgc gacggcggtg cccgagcctt 60ttcctcctct tagaaaccca cttctgaaag gtataaaaac atg act att cga atc 115
Met Thr Ile Arg Ile
1 5gga ctc gtt ggc tac ggt gtc ggc ggc agg ctc ttt cac acc cct tac 163Gly Leu Val Gly Tyr Gly Val Gly Gly Arg Leu Phe His Thr Pro Tyr
10 15 20atc caa gct tct acg cac tgc gaa tta gta ggc gta gtt gct cgt tcc 211Ile Gln Ala Ser Thr His Cys Glu Leu Val Gly Val Val Ala Arg Ser
25 30 35gaa ggc acc aaa gca gcc gtt gca gaa gat ctt cca gat gtt gcc atc 259Glu Gly Thr Lys Ala Ala Val Ala Glu Asp Leu Pro Asp Val Ala Ile
40 45 50gtg gga tcg ctg aca gaa ctc ctc gaa ctg ggc gtc gat gca gtg gtg 307Val Gly Ser Leu Thr Glu Leu Leu Glu Leu Gly Val Asp Ala Val Val
55 60 65atc tcc acc cct cca gcc acg cgc cgg gaa ctg gcc ttg gaa gca atc 355Ile Ser Thr Pro Pro Ala Thr Arg Arg Glu Leu Ala Leu Glu Ala Ile70 75 80 85aac gca ggt gtc gca gtg gtt gcc gat aaa ccg ttt gca cca tca gcc 403Asn Ala Gly Val Ala Val Val Ala Asp Lys Pro Phe Ala Pro Ser Ala
90 95 100gca gat gcc atg gaa ctt gtc gaa gcc gcc gaa aag gct gga gtg ctg 451Ala Asp Ala Met Glu Leu Val Glu Ala Ala Glu Lys Ala Gly Val Leu
105 110 115ctc aac gtc ttc cac aac agg cgc aac gac acc cac att gtc acg gca 499Leu Asn Val Phe His Asn Arg Arg Asn Asp Thr His Ile Val Thr Ala
120 125 130ctg gga atc caa gaa gaa ctt ggt gcg atg cgt gga ctg gac ctg cga 547Leu Gly Ile Gln Glu Glu Leu Gly Ala Met Arg Gly Leu Asp Leu Arg
135 140 145cta gac ctg atc gaa cct gat tcc ttg gag gca ggt cct gaa ggt ggt 595Leu Asp Leu Ile Glu Pro Asp Ser Leu Glu Ala Gly Pro Glu Gly Gly150 155 160 165ttg ctg cgc gat ctg ggc tca cac gta gtc gat cag acc ctg gtt ctc 643Leu Leu Arg Asp Leu Gly Ser His Val Val Asp Gln Thr Leu Val Leu
170 175 180atg ggg ccg gct acc tct gtc acc gcc caa ctt gga tcc atc gac ctt 691Met Gly Pro Ala Thr Ser Val Thr Ala Gln Leu Gly Ser Ile Asp Leu
185 190 195cca gaa ggc cca acc aac gca agg ttc cgc atc gtg ttg gaa cat gaa 739Pro Glu Gly Pro Thr Asn Ala Arg Phe Arg Ile Val Leu Glu His Glu
200 205 210tcg ggc gcc gta tcg cac att tct gcc agc aag att gac cgc ttg gag 787Ser Gly Ala Val Ser His Ile Ser Ala Ser Lys Ile Asp Arg Leu Glu
215 220 225tcc tgg gaa atc cgc ttg gtg ggc gag cgc ggc tcc tac gta tcc aac 835Ser Trp Glu Ile Arg Leu Val Gly Glu Arg Gly Ser Tyr Val Ser Asn230 235 240 245tac acc gac gtg cag acc gtg gcg atc aaa cag gga ctt cga cca acc 883Tyr Thr Asp Val Gln Thr Val Ala Ile Lys Gln Gly Leu Arg Pro Thr
250 255 260aat gac cgc gaa cac tgg ggc tac gaa tcg gag gag cgg tgg ggc acc 931Asn Asp Arg Glu His Trp Gly Tyr Glu Ser Glu Glu Arg Trp Gly Thr
265 270 275ttg gtt acc gat gaa ggc tca aag gtg att cct tca gca caa ggc gat 979Leu Val Thr Asp Glu Gly Ser Lys Val Ile Pro Ser Ala Gln Gly Asp
280 285 290tac acc cgc ttc tac gat gcc ttt gcc ttg gct gtg gaa aac ggt ggc 1027Tyr Thr Arg Phe Tyr Asp Ala Phe Ala Leu Ala Val Glu Asn Gly Gly
295 300 305gca ggg ccg gtg cct gca cgt gaa ggt gtt gca gtg ctc aag gtg ttg 1075Ala Gly Pro Val Pro Ala Arg Glu Gly Val Ala Val Leu Lys Val Leu310 315 320 325gat gct gta gcc cag agc gct gcg gaa aaa cgc acc att gag ttg agc 1123Asp Ala Val Ala Gln Ser Ala Ala Glu Lys Arg Thr Ile Glu Leu Ser
330 335 340taaggagaag tgctgctggc tgc 1146<210>304<211>341<212>PRT<213>谷氨酸棒杆菌<400>304Met Thr Ile Arg Ile Gly Leu Val Gly Tyr Gly Val Gly Gly Arg Leu1 5 10 15Phe His Thr Pro Tyr Ile Gln Ala Ser Thr His Cys Glu Leu Val Gly
20 25 30Val Val Ala Arg Ser Glu Gly Thr Lys Ala Ala Val Ala Glu Asp Leu
35 40 45Pro Asp Val Ala Ile Val Gly Ser Leu Thr Glu Leu Leu Glu Leu Gly
50 55 60Val Asp Ala Val Val Ile Ser Thr Pro Pro Ala Thr Arg Arg Glu Leu65 70 75 80Ala Leu Glu Ala Ile Asn Ala Gly Val Ala Val Val Ala Asp Lys Pro
85 90 95Phe Ala Pro Ser Ala Ala Asp Ala Met Glu Leu Val Glu Ala Ala Glu
100 105 110Lys Ala Gly Val Leu Leu Asn Val Phe His Asn Arg Arg Asn Asp Thr
115 120 125His Ile Val Thr Ala Leu Gly Ile Gln Glu Glu Leu Gly Ala Met Arg
130 135 140Gly Leu Asp Leu Arg Leu Asp Leu Ile Glu Pro Asp Ser Leu Glu Ala145 150 155 160Gly Pro Glu Gly Gly Leu Leu Arg Asp Leu Gly Ser His Val Val Asp
165 170 175Gln Thr Leu Val Leu Met Gly Pro Ala Thr Ser Val Thr Ala Gln Leu
180 185 190Gly Ser Ile Asp Leu Pro Glu Gly Pro Thr Asn Ala Arg Phe Arg Ile
195 200 205Val Leu Glu His Glu Ser Gly Ala Val Ser His Ile Ser Ala Ser Lys
210 215 220Ile Asp Arg Leu Glu Ser Trp Glu Ile Arg Leu Val Gly Glu Arg Gly225 230 235 240Ser Tyr Val Ser Asn Tyr Thr Asp Val Gln Thr Val Ala Ile Lys Gln
245 250 255Gly Leu Arg Pro Thr Asn Asp Arg Glu His Trp Gly Tyr Glu Ser Glu
260 265 270Glu Arg Trp Gly Thr Leu Val Thr Asp Glu Gly Ser Lys Val Ile Pro
275 280 285Ser Ala Gln Gly Asp Tyr Thr Arg Phe Tyr Asp Ala Phe Ala Leu Ala
290 295 300Val Glu Asn Gly Gly Ala Gly Pro Val Pro Ala Arg Glu Gly Val Ala305 310 315 320Val Leu Lys Val Leu Asp Ala Val Ala Gln Ser Ala Ala Glu Lys Arg
325 330 335Thr Ile Glu Leu Ser
340<210>305<211>1200<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1177)<223> RXN01630<400>305gtaggtgagt cttcgtgaga tacccccggc cagtcataca gttcaaccaa gctccaccac 60ccagataaaa acctgcgggt tgcgttttag gagaattccc atg agt gat caa aaa 115
Met Ser Asp Gln Lys
1 5att gtt gtt ggc ctg cta ggc atc acc cac ccg cat gcg tcg gcg cgg 163Ile Val Val Gly Leu Leu Gly Ile Thr His Pro His Ala Ser Ala Arg
10 15 20gtg cgt gcc ctc cgt gaa att gat ggg gta gag gtc gtc gcc gcc gcg 211Val Arg Ala Leu Arg Glu Ile Asp Gly Val Glu Val Val Ala Ala Ala
25 30 35gat act gat tcc cgc ctc cag tac ttc acc gac aaa tat gat gtt gaa 259Asp Thr Asp Ser Arg Leu Gln Tyr Phe Thr Asp Lys Tyr Asp Val Glu
40 45 50ccc cgc gag atc gat gac gtc ttg aac gac gat cgc atc aac gcc atc 307Pro Arg Glu Ile Asp Asp Val Leu Asn Asp Asp Arg Ile Asn Ala Ile
55 60 65atg gtt cac tcc aag agc aag gac atg gtc cct cac gcc aag cgc gcg 355Met Val His Ser Lys Ser Lys Asp Met Val Pro His Ala Lys Arg Ala70 75 80 85ctc gcg gcc gga aaa tcc gtc gtc gtg gag aag ccc ggc ggg gga aca 403Leu Ala Ala Gly Lys Ser Val Val Val Glu Lys Pro Gly Gly Gly Thr
90 95 100gtg gcg gat ctt gag gag ctc ctg gcc ctc aaa gaa gct gcc gat cct 451Val Ala Asp Leu Glu Glu Leu Leu Ala Leu Lys Glu Ala Ala Asp Pro
105 110 115cag cga atc gtg cag gtc ggg tac aac gtc cgc ctg tct gaa tcg gtt 499Gln Arg Ile Val Gln Val Gly Tyr Asn Val Arg Leu Ser Glu Ser Val
120 125 130cag aga tta aaa gag ctt ctc gac gcc ggc ctc atc ggc gaa gtc gtc 547Gln Arg Leu Lys Glu Leu Leu Asp Ala Gly Leu Ile Gly Glu Val Val
135 140 145agc gtg caa gca cgc ggc gcc gca aaa gta ggt gag cat atc acc gag 595Ser Val Gln Ala Arg Gly Ala Ala Lys Val Gly Glu His Ile Thr Glu150 155 160 165cac ctc aac caa ccc gca gac atg ggc ggt gtg ttg tgg att ctt ggc 643His Leu Asn Gln Pro Ala Asp Met Gly Gly Val Leu Trp Ile Leu Gly
170 175 180tgc cac atg ctc gat gca ttg gtg gaa gtc ttc gga gct cca gaa tcc 691Cys His Met Leu Asp Ala Leu Val Glu Val Phe Gly Ala Pro Glu Ser
185 190 195gtg aac gcc cga gtg cat aag acc gca aaa ctc tct gac gac acc agc 739Val Asn Ala Arg Val His Lys Thr Ala Lys Leu Ser Asp Asp Thr Ser
200 205 210cgc gaa gac tca gcc tcc gca ctg ctg tac tac cca gat ttc tcc gtc 787Arg Glu Asp Ser Ala Ser Ala Leu Leu Tyr Tyr Pro Asp Phe Ser Val
215 220 225agc ttc agc ttc gac ggc cac gat gat ctg gaa tgg ttc gaa agc tcc 835Ser Phe Ser Phe Asp Gly His Asp Asp Leu Glu Trp Phe Glu Ser Ser230 235 240 245cga ctc acg gtc tat gga acc aag ggc atg atc gaa gcc gga atc ctc 883Arg Leu Thr Val Tyr Gly Thr Lys Gly Met Ile Glu Ala Gly Ile Leu
250 255 260cct cag aca ctg cgc gta tac ctc aat gag tca cgc cag ggc tgg cca 931Pro Gln Thr Leu Arg Val Tyr Leu Asn Glu Ser Arg Gln Gly Trp Pro
265 270 275cag ggt tgg acc gag tgg acc cag agc tac ttc acc cca ccg ttt gct 979Gln Gly Trp Thr Glu Trp Thr Gln Ser Tyr Phe Thr Pro Pro Phe Ala
280 285 290cgc aca gaa tcc aac aaa ttc tca gag ctt cca gag cta gaa aac atc 1027Arg Thr Glu Ser Asn Lys Phe Ser Glu Leu Pro Glu Leu Glu Asn Ile
295 300 305agc aac ttc cgc aca gaa atg cag ggg tgg gtg aat tcc att cgc act 1075Ser Asn Phe Arg Thr Glu Met Gln Gly Trp Val Asn Ser Ile Arg Thr310 315 320 325gga tcc cgc aat gtg gcg cca gtt gag gat gct ctc aca gtc gct cgc 1123Gly Ser Arg Asn Val Ala Pro Val Glu Asp Ala Leu Thr Val Ala Arg
330 335 340att gtc agt gca tgc tac gaa tcc gac aac aac cag ggc att tcc gta 1171Ile Val Ser Ala Cys Tyr Glu Ser Asp Asn Asn Gln Gly Ile Ser Val
345 350 355aac atc taagaggagc actccatgaa acc 1200Asn Ile<210>306<211>359<212>PRT<213>谷氨酸棒杆菌<400>306Met Ser Asp Gln Lys Ile Val Val Gly Leu Leu Gly Ile Thr His Pro1 5 10 15His Ala Ser Ala Arg Val Arg Ala Leu Arg Glu Ile Asp Gly Val Glu
20 25 30Val Val Ala Ala Ala Asp Thr Asp Ser Arg Leu Gln Tyr Phe Thr Asp
35 40 45Lys Tyr Asp Val Glu Pro Arg Glu Ile Asp Asp Val Leu Asn Asp Asp
50 55 60Arg Ile Asn Ala Ile Met Val His Ser Lys Ser Lys Asp Met Val Pro65 70 75 80His Ala Lys Arg Ala Leu Ala Ala Gly Lys Ser Val Val Val Glu Lys
85 90 95Pro Gly Gly Gly Thr Val Ala Asp Leu Glu Glu Leu Leu Ala Leu Lys
100 105 110Glu Ala Ala Asp Pro Gln Arg Ile Val Gln Val Gly Tyr Asn Val Arg
115 120 125Leu Ser Glu Ser Val Gln Arg Leu Lys Glu Leu Leu Asp Ala Gly Leu
130 135 140Ile Gly Glu Val Val Ser Val Gln Ala Arg Gly Ala Ala Lys Val Gly145 150 155 160Glu His Ile Thr Glu His Leu Asn Gln Pro Ala Asp Met Gly Gly Val
165 170 175Leu Trp Ile Leu Gly Cys His Met Leu Asp Ala Leu Val Glu Val Phe
180 185 190Gly Ala Pro Glu Ser Val Asn Ala Arg Val His Lys Thr Ala Lys Leu
195 200 205Ser Asp Asp Thr Ser Arg Glu Asp Ser Ala Ser Ala Leu Leu Tyr Tyr
210 215 220Pro Asp Phe Ser Val Ser Phe Ser Phe Asp Gly His Asp Asp Leu Glu225 230 235 240Trp Phe Glu Ser Ser Arg Leu Thr Val Tyr Gly Thr Lys Gly Met Ile
245 250 255Glu Ala Gly Ile Leu Pro Gln Thr Leu Arg Val Tyr Leu Asn Glu Ser
260 265 270Arg Gln Gly Trp Pro Gln Gly Trp Thr Glu Trp Thr Gln Ser Tyr Phe
275 280 285Thr Pro Pro Phe Ala Arg Thr Glu Ser Asn Lys Phe Ser Glu Leu Pro
290 295 300Glu Leu Glu Asn Ile Ser Asn Phe Arg Thr Glu Met Gln Gly Trp Val305 310 315 320Asn Ser Ile Arg Thr Gly Ser Arg Asn Val Ala Pro Val Glu Asp Ala
325 330 335Leu Thr Val Ala Arg Ile Val Ser Ala Cys Tyr Glu Ser Asp Asn Asn
340 345 350Gln Gly Ile Ser Val Asn Ile
355<210>307<211>1212<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1189)<223>RXN00528<400>307ttctgctggg aatccccaca ttttggaacg tagcgtcgat aagcgtgcgg cgaagctttt 60tcggtcgcgg ccgttatctt tttaagagga gaaattttag atg agc acg tcc acc 115
Met Ser Thr Ser Thr
1 5atc agg gtt gcc att gcc gga gtc gga aac tgc gcg acc tcc ctc att 163Ile Arg Val Ala Ile Ala Gly Val Gly Asn Cys Ala Thr Ser Leu Ile
10 15 20cag ggt gtg gaa tat tac cga aat gcg gat cct tcc gaa act gtc ccg 211Gln Gly Val Glu Tyr Tyr Arg Asn Ala Asp Pro Ser Glu Thr Val Pro
25 30 35ggt ttg atg cac gtc aaa ttc ggt gat tac cac gtt ggc gac att gaa 259Gly Leu Met His Val Lys Phe Gly Asp Tyr His Val Gly Asp Ile Glu
40 45 50ttc gtg gcc gcg ttc gac gtc gac gcc gaa aaa gta ggc atc gat ctt 307Phe Val Ala Ala Phe Asp Val Asp Ala Glu Lys Val Gly Ile Asp Leu
55 60 65gcc gac gcc acc gag gct tca caa aac tgc act atc aaa atc gcc gat 355Ala Asp Ala Thr Glu Ala Ser Gln Asn Cys Thr Ile Lys Ile Ala Asp70 75 80 85gtc cca cag acc ggc atc aac gtg ctg cgt ggc ccg act ctc gac ggc 403Val Pro Gln Thr Gly Ile Asn Val Leu Arg Gly Pro Thr Leu Asp Gly
90 95 100ctg ggc gat cat tac cgc gcg acc atc gac gag tcc acc gcc gag cca 451Leu Gly Asp His Tyr Arg Ala Thr Ile Asp Glu Ser Thr Ala Glu Pro
105 110 115gtc gac gtt gtc cag gcg ctt atc gac gca aaa gcc gat gtt ttg gtg 499Val Asp Val Val Gln Ala Leu Ile Asp Ala Lys Ala Asp Val Leu Val
120 125 130tcc tac ctc cca gtg ggc tcc gaa gaa gcc gac aaa ttc tac gca caa 547Ser Tyr Leu Pro Val Gly Ser Glu Glu Ala Asp Lys Phe Tyr Ala Gln
135 140 145gcc gcc atc gat gca ggc tgc gcc ttt gtc aac gct ctc cca gta ttc 595Ala Ala Ile Asp Ala Gly Cys Ala Phe Val Asn Ala Leu Pro Val Phe150 155 160 165atc gcc tcc gac cct gag tgg gct aag aag ttc act gac gct ggc atc 643Ile Ala Ser Asp Pro Glu Trp Ala Lys Lys Phe Thr Asp Ala Gly Ile
170 175 180cca att gtt ggc gat gac atc aaa tcc cag atc ggt gca acc atc acc 691Pro Ile Val Gly Asp Asp Ile Lys Ser Gln Ile Gly Ala Thr Ile Thr
185 190 195cac cgt gtc ctc gca cgc ctt ttt gaa gaa cgt ggc gtt cgc gta gat 739His Arg Val Leu Ala Arg Leu Phe Glu Glu Arg Gly Val Arg Val Asp
200 205 210cgc acc atg cag ctc aac gtc ggc ggc aac atg gac ttc aaa aac atg 787Arg Thr Met Gln Leu Asn Val Gly Gly Asn Met Asp Phe Lys Asn Met
215 220 225ctt gac cgc aat cgc ttg gaa tcc aag aag gtc tcc aaa acc caa gca 835Leu Asp Arg Asn Arg Leu Glu Ser Lys Lys Val Ser Lys Thr Gln Ala230 235 240 245gtg acc tcc aac att cca gat ggt cca ctg tct gga aag gtg gaa gac 883Val Thr Ser Asn Ile Pro Asp Gly Pro Leu Ser Gly Lys Val Glu Asp
250 255 260cgc aac gtc cac atc gga cca tcc gac cac gtc caa tgg ctc gat gac 931Arg Asn Val His Ile Gly Pro Ser Asp His Val Gln Trp Leu Asp Asp
265 270 275cgc aag tgg gct tat gtc cgc ctc gaa ggc acc gca ttc ggt gga gtt 979Arg Lys Trp Ala Tyr Val Arg Leu Glu Gly Thr Ala Phe Gly Gly Val
280 285 290ccc ctc aac ctt gag tac aaa ctc gag gtg tgg gat tca ccc aac tct 1027Pro Leu Asn Leu Glu Tyr Lys Leu Glu Val Trp Asp Ser Pro Asn Ser
295 300 305gcc ggc atc atc atc gac gct gtt cgc gcc gcc aag atc gcc ctc gat 1075Ala Gly Ile Ile Ile Asp Ala Val Arg Ala Ala Lys Ile Ala Leu Asp310 315 320 325cgc ggt atc ggc gga ccg atc atg cca gca agc tcc tac ctg atg aag 1123Arg Gly Ile Gly Gly Pro Ile Met Pro Ala Ser Ser Tyr Leu Met Lys
330 335 340tcc cca cct gag cag ctt cca gac gat gtt gct tgt gaa cgc cta gag 1171Ser Pro Pro Glu Gln Leu Pro Asp Asp Val Ala Cys Glu Arg Leu Glu
345 350 355gca ttc atc atc gag gcg taaaattagg ctaaaaattt ggg 1212Ala Phe Ile Ile Glu Ala
360<210>308<211>363<212>PRT<213>谷氨酸棒杆菌<400>308Met Ser Thr Ser Thr Ile Arg Val Ala Ile Ala Gly Val Gly Asn Cys1 5 10 15Ala Thr Ser Leu Ile Gln Gly Val Glu Tyr Tyr Arg Asn Ala Asp Pro
20 25 30Ser Glu Thr Val Pro Gly Leu Met His Val Lys Phe Gly Asp Tyr His
35 40 45Val Gly Asp Ile Glu Phe Val Ala Ala Phe Asp Val Asp Ala Glu Lys
50 55 60Val Gly Ile Asp Leu Ala Asp Ala Thr Glu Ala Ser Gln Asn Cys Thr65 70 75 80Ile Lys Ile Ala Asp Val Pro Gln Thr Gly Ile Asn Val Leu Arg Gly
85 90 95Pro Thr Leu Asp Gly Leu Gly Asp His Tyr Arg Ala Thr Ile Asp Glu
100 105 110Ser Thr Ala Glu Pro Val Asp Val Val Gln Ala Leu Ile Asp Ala Lys
115 120 125Ala Asp Val Leu Val Ser Tyr Leu Pro Val Gly Ser Glu Glu Ala Asp
130 135 140Lys Phe Tyr Ala Gln Ala Ala Ile Asp Ala Gly Cys Ala Phe Val Asn145 150 155 160Ala Leu Pro Val Phe Ile Ala Ser Asp Pro Glu Trp Ala Lys Lys Phe
165 170 175Thr Asp Ala Gly Ile Pro Ile Val Gly Asp Asp Ile Lys Ser Gln Ile
180 185 190Gly Ala Thr Ile Thr His Arg Val Leu Ala Arg Leu Phe Glu Glu Arg
195 200 205Gly Val Arg Val Asp Arg Thr Met Gln Leu Asn Val Gly Gly Asn Met
210 215 220Asp Phe Lys Asn Met Leu Asp Arg Asn Arg Leu Glu Ser Lys Lys Val225 230 235 240Ser Lys Thr Gln Ala Val Thr Ser Asn Ile Pro Asp Gly Pro Leu Ser
245 250 255Gly Lys Val Glu Asp Arg Asn Val His Ile Gly Pro Ser Asp His Val
260 265 270Gln Trp Leu Asp Asp Arg Lys Trp Ala Tyr Val Arg Leu Glu Gly Thr
275 280 285Ala Phe Gly Gly Val Pro Leu Asn Leu Glu Tyr Lys Leu Glu Val Trp
290 295 300Asp Ser Pro Asn Ser Ala Gly Ile Ile Ile Asp Ala Val Arg Ala Ala305 310 315 320Lys Ile Ala Leu Asp Arg Gly Ile Gly Gly Pro Ile Met Pro Ala Ser
325 330 335Ser Tyr Leu Met Lys Ser Pro Pro Glu Gln Leu Pro Asp Asp Val Ala
340 345 350Cys Glu Arg Leu Glu Ala Phe Ile Ile Glu Ala
355 360<210>309<211>795<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(772)<223>RXN03057<400>309catcaacgcc gagtacaact aaggacaact gataatgaca aatgctgcaa ttgtcggatg 60aggagacgtc gcaaccgttc atacagaagc gctggaagct ttg gct tcc gat ctt 115
Leu Ala Ser Asp Leu
1 5ggt att aag ttc gtc gca gtg gtg gat aaa gat cta gag act gct gag 163Gly Ile Lys Phe Val Ala Val Val Asp Lys Asp Leu Glu Thr Ala Glu
10 15 20aaa ttt gcg acg gga ctt gga gct gct ggc gat tct tca gaa agc agc 211Lys Phe Ala Thr Gly Leu Gly Ala Ala Gly Asp Ser Ser Glu Ser Ser
25 30 35gtc aag gcc cac ggc agc ctg ccg gct ttg ttc tcc aaa aag aag atc 259Val Lys Ala His Gly Ser Leu Pro Ala Leu Phe Ser Lys Lys Lys Ile
40 45 50gat gtt cta cac atc acc acc ccc cac gac caa cac att ggt ttg gct 307Asp Val Leu His Ile Thr Thr Pro His Asp Gln His Ile Gly Leu Ala
55 60 65ctc gaa gcg cta cac cac ggt gta aat gtc atc ctg gaa aag ccg ttg 355Leu Glu Ala Leu His His Gly Val Asn Val Ile Leu Glu Lys Pro Leu70 75 80 85gct aat gag ttg gac cag gcg cag cgt ctc atc gac tac ttg gat gaa 403Ala Asn Glu Leu Asp Gln Ala Gln Arg Leu Ile Asp Tyr Leu Asp Glu
90 95 100aac ccc gat ggt cca aag att gca gtg tgc tat cag aac cgt tac aac 451Asn Pro Asp Gly Pro Lys Ile Ala Val Cys Tyr Gln Asn Arg Tyr Asn
105 110 115gtt tcc tcc cag gaa ctg cgt cgt ctg ctc gat tca ggt gac ctc ggt 499Val Ser Ser Gln Glu Leu Arg Arg Leu Leu Asp Ser Gly Asp Leu Gly
120 125 130gcc atc aat ggt gca tat tcc tct gtg gtg tgg acc cgc acc cca ggc 547Ala Ile Asn Gly Ala Tyr Ser Ser Val Val Trp Thr Arg Thr Pro Gly
135 140 145tac tac acc cag aaa cct tgg cgt ggc cag caa gca cac tcc ggt ggt 595Tyr Tyr Thr Gln Lys Pro Trp Arg Gly Gln Gln Ala His Ser Gly Gly150 155 160 165ggc ctg ctg atg aac caa gca att cac acc ctg gat ctg ctg cag tgg 643Gly Leu Leu Met Asn Gln Ala Ile His Thr Leu Asp Leu Leu Gln Trp
170 175 180ttc ctt gga aag gca aca gaa gtc aag ggc act gtc tcc acc gat aag 691Phe Leu Gly Lys Ala Thr Glu Val Lys Gly Thr Val Ser Thr Asp Lys
185 190 195tat gcc gat gtc atc gat gtt gaa gac acc gca cac gca tac atc ggt 739Tyr Ala Asp Val Ile Asp Val Glu Asp Thr Ala His Ala Tyr Ile Gly
200 205 210cac gag tcc gga gtc cac acc agt gaa gtg agt tgaaccatgc tattggtgat 792His Glu Ser Gly Val His Thr Ser Glu Val Ser
215 220aca 795<210>310<211>224<212>PRT<213>谷氨酸棒杆菌<400>310Leu Ala Ser Asp Leu Gly Ile Lys Phe Val Ala Val Val Asp Lys Asp1 5 10 15Leu Glu Thr Ala Glu Lys Phe Ala Thr Gly Leu Gly Ala Ala Gly Asp
20 25 30Ser Ser Glu Ser Ser Val Lys Ala His Gly Ser Leu Pro Ala Leu Phe
35 40 45Ser Lys Lys Lys Ile Asp Val Leu His Ile Thr Thr Pro His Asp Gln
50 55 60His Ile Gly Leu Ala Leu Glu Ala Leu His His Gly Val Asn Val Ile65 70 75 80Leu Glu Lys Pro Leu Ala Asn Glu Leu Asp Gln Ala Gln Arg Leu Ile
85 90 95Asp Tyr Leu Asp Glu Asn Pro Asp Gly Pro Lys Ile Ala Val Cys Tyr
100 105 110Gln Asn Arg Tyr Asn Val Ser Ser Gln Glu Leu Arg Arg Leu Leu Asp
115 120 125Ser Gly Asp Leu Gly Ala Ile Asn Gly Ala Tyr Ser Ser Val Val Trp
130 135 140Thr Arg Thr Pro Gly Tyr Tyr Thr Gln Lys Pro Trp Arg Gly Gln Gln145 150 155 160Ala His Ser Gly Gly Gly Leu Leu Met Asn Gln Ala Ile His Thr Leu
165 170 175Asp Leu Leu Gln Trp Phe Leu Gly Lys Ala Thr Glu Val Lys Gly Thr
180 185 190Val Ser Thr Asp Lys Tyr Ala Asp Val Ile Asp Val Glu Asp Thr Ala
195 200 205His Ala Tyr Ile Gly His Glu Ser Gly Val His Thr Ser Glu Val Ser
210 215 220<210>311<211>795<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(772)<223>FRXA02902<400>311catcaacgcc gagtacaact aaggacaact gataatgaca aatgctgcaa ttgtcggatg 60aggagacgtc gcaaccgttc atacagaagc gctggaagct ttg gct tcc gat ctt 115
Leu Ala Ser Asp Leu
1 5ggt att aag ttc gtc gca gtg gtg gat aaa gat cta gag act gct gag 163Gly Ile Lys Phe Val Ala Val Val Asp Lys Asp Leu Glu Thr Ala Glu
10 15 20aaa ttt gcg acg gga ctt gga gct gct ggc gat tct tca gaa agc agc 211Lys Phe Ala Thr Gly Leu Gly Ala Ala Gly Asp Ser Ser Glu Ser Ser
25 30 35gtc aag gcc cac ggc agc ctg ccg gct ttg ttc tcc aaa aag aag atc 259Val Lys Ala His Gly Ser Leu Pro Ala Leu Phe Ser Lys Lys Lys Ile
40 45 50gat gtt cta cac atc acc acc ccc cac gac caa cac att ggt ttg gct 307Asp Val Leu His Ile Thr Thr Pro His Asp Gln His Ile Gly Leu Ala
55 60 65ctc gaa gcg cta cac cac ggt gta aat gtc atc ctg gaa aag ccg ttg 355Leu Glu Ala Leu His His Gly Val Asn Val Ile Leu Glu Lys Pro Leu70 75 80 85gct aat gag ttg gac cag gcg cag cgt ctc atc gac tac ttg gat gaa 403Ala Asn Glu Leu Asp Gln Ala Gln Arg Leu Ile Asp Tyr Leu Asp Glu
90 95 100aac ccc gat ggt cca aag att gca gtg tgc tat cag aac cgt tac aac 451Asn Pro Asp Gly Pro Lys Ile Ala Val Cys Tyr Gln Asn Arg Tyr Asn
105 110 115gtt tcc tcc cag gaa ctg cgt cgt ctg ctc gat tca ggt gac ctc ggt 499Val Ser Ser Gln Glu Leu Arg Arg Leu Leu Asp Ser Gly Asp Leu Gly
120 125 130gcc atc aat ggt gca tat tcc tct gtg gtg tgg acc cgc acc cca ggc 547Ala Ile Asn Gly Ala Tyr Ser Ser Val Val Trp Thr Arg Thr Pro Gly
135 140 145tac tac acc cag aaa cct tgg cgt ggc cag caa gca cac tcc ggt ggt 595Tyr Tyr Thr Gln Lys Pro Trp Arg Gly Gln Gln Ala His Ser Gly Gly150 155 160 165ggc ctg ctg atg aac caa gca att cac acc ctg gat ctg ctg cag tgg 643Gly Leu Leu Met Asn Gln Ala Ile His Thr Leu Asp Leu Leu Gln Trp
170 175 180ttc ctt gga aag gca aca gaa gtc aag ggc act gtc tcc acc gat aag 691Phe Leu Gly Lys Ala Thr Glu Val Lys Gly Thr Val Ser Thr Asp Lys
185 190 195tat gcc gat gtc atc gat gtt gaa gac acc gca cac gca tac atc ggt 739Tyr Ala Asp Val Ile Asp Val Glu Asp Thr Ala His Ala Tyr Ile Gly
200 205 210cac gag tcc gga gtc cac acc agt gaa gtg agt tgaaccatgc tattggtgat 792His Glu Ser Gly Val His Thr Ser Glu Val Ser
215 220aca 795<210>312<211>224<212>PRT<213>谷氨酸棒杆菌<400>312Leu Ala Ser Asp Leu Gly Ile Lys Phe Val Ala Val Val Asp Lys Asp1 5 10 15Leu Glu Thr Ala Glu Lys Phe Ala Thr Gly Leu Gly Ala Ala Gly Asp
20 25 30Ser Ser Glu Ser Ser Val Lys Ala His Gly Ser Leu Pro Ala Leu Phe
35 40 45Ser Lys Lys Lys Ile Asp Val Leu His Ile Thr Thr Pro His Asp Gln
50 55 60His Ile Gly Leu Ala Leu Glu Ala Leu His His Gly Val Asn Val Ile65 70 75 80Leu Glu Lys Pro Leu Ala Asn Glu Leu Asp Gln Ala Gln Arg Leu Ile
85 90 95Asp Tyr Leu Asp Glu Asn Pro Asp Gly Pro Lys Ile Ala Val Cys Tyr
100 105 110Gln Asn Arg Tyr Asn Val Ser Ser Gln Glu Leu Arg Arg Leu Leu Asp
115 120 125Ser Gly Asp Leu Gly Ala Ile Asn Gly Ala Tyr Ser Ser Val Val Trp
130 135 140Thr Arg Thr Pro Gly Tyr Tyr Thr Gln Lys Pro Trp Arg Gly Gln Gln145 150 155 160Ala His Ser Gly Gly Gly Leu Leu Met Asn Gln Ala Ile His Thr Leu
165 170 175Asp Leu Leu Gln Trp Phe Leu Gly Lys Ala Thr Glu Val Lys Gly Thr
180 185 190Val Ser Thr Asp Lys Tyr Ala Asp Val Ile Asp Val Glu Asp Thr Ala
195 200 205His Ala Tyr Ile Gly His Glu Ser Gly Val His Thr Ser Glu Val Ser
210 215 220<210>313<211>831<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(808)<223>RXA00251<400>313aaccagcgtt ttcagcgaga tactggacat atcaactaaa atccctgaat aaaacatcta 60acatgggttt tatacagaaa attcatacga aaggttgatc atg aag aag aag att 115
Met Lys Lys Lys Ile
1 5gcg gtc gtt acc gga gcg acc gga ggc atg gga att gag atc gtc aaa 163Ala Val Val Thr Gly Ala Thr Gly Gly Met Gly Ile Glu Ile Val Lys
10 15 20gac ctc tcc cgc gac cac att gtc tac gcc ttg ggc cga aat cca gag 211Asp Leu Ser Arg Asp His Ile Val Tyr Ala Leu Gly Arg Asn Pro Glu
25 30 35cat ctg gca gct ctc gca gag atc gag gga gta gag cct atc gag tcc 259His Leu Ala Ala Leu Ala Glu Ile Glu Gly Val Glu Pro Ile Glu Ser
40 45 50gat atc gtg aag gaa gtg ttg gaa gag gga ggc gtc gac aag cta aaa 307Asp Ile Val Lys Glu Val Leu Glu Glu Gly Gly Val Asp Lys Leu Lys
55 60 65aac ctc gac cac gtg gat acg ctg gtg cac gcc gcg gcg gtg gcg cgt 355Asn Leu Asp His Val Asp Thr Leu Val His Ala Ala Ala Val Ala Arg70 75 80 85gac acg acc atc gaa gcc ggc agt gtg gcc gaa tgg cac gca cac ctt 403Asp Thr Thr Ile Glu Ala Gly Ser Val Ala Glu Trp Hls Ala His Leu
90 95 100gat ctc aac gtc att gtc ccg gcc gag ttg agt cgc caa ctc ttg ccc 451Asp Leu Asn Val Ile Val Pro Ala Glu Leu Ser Arg Gln Leu Leu Pro
105 110 115gcc ctc cgc gcg gca tcc ggc tgc gtc atc tac atc aac tcc ggc gcc 499Ala Leu Arg Ala Ala Ser Gly Cys Val Ile Tyr Ile Asn Ser Gly Ala
120 125 130ggc aac gga cca cac ccc ggc aac acc atc tac gcc gcc agc aaa cac 547Gly Asn Gly Pro His Pro Gly Asn Thr Ile Tyr Ala Ala Ser Lys His
135 140 145gcc ctc cgc gga ctc gcc gac gcc ttc cgc aaa gaa gaa gcc aac aac 595Ala Leu Arg Gly Leu Ala Asp Ala Phe Arg Lys Glu Glu Ala Asn Asn150 155 160 165ggc atc cgc gtc agc act gtc agc ccc ggc ccc acc aac acc ccc atg 643Gly Ile Arg Val Ser Thr Val Ser Pro Gly Pro Thr Asn Thr Pro Met
170 175 180ctg caa ggc ctc atg gac tca caa ggc acc aac ttc cgc cca gag atc 691Leu Gln Gly Leu Met Asp Ser Gln Gly Thr Asn Phe Arg Pro Glu Ile
185 190 195tac atc gaa cca aaa gaa atc gcc aac gca atc aga ttc gtg att gac 739Tyr Ile Glu Pro Lys Glu Ile Ala Asn Ala Ile Arg Phe Val Ile Asp
200 205 210gct ggc gaa acc acc cag atc acc aac gtg gac gta cga cca cgt atc 787Ala Gly Glu Thr Thr Gln Ile Thr Asn Val Asp Val Arg Pro Arg Ile
215 220 225gaa ctg gcg gac cgg aaa gat tagttctggg gggcttcctg ggc 831Glu Leu Ala Asp Arg Lys Asp230 235<210>314<211>236<212>PRT<213>谷氨酸棒杆菌<400>314Met Lys Lys Lys Ile Ala Val Val Thr Gly Ala Thr Gly Gly Met Gly1 5 10 15Ile Glu Ile Val Lys Asp Leu Ser Arg Asp His Ile Val Tyr Ala Leu
20 25 30Gly Arg Asn Pro Glu His Leu Ala Ala Leu Ala Glu Ile Glu Gly Val
35 40 45Glu Pro Ile Glu Ser Asp Ile Val Lys Glu Val Leu Glu Glu Gly Gly
50 55 60Val Asp Lys Leu Lys Asn Leu Asp His Val Asp Thr Leu Val His Ala65 70 75 80Ala Ala Val Ala Arg Asp Thr Thr Ile Glu Ala Gly Ser Val Ala Glu
85 90 95Trp His Ala His Leu Asp Leu Asn Val Ile Val Pro Ala Glu Leu Ser
100 105 110Arg Gln Leu Leu Pro Ala Leu Arg Ala Ala Ser Gly Cys Val Ile Tyr
115 120 125Ile Asn Ser Gly Ala Gly Asn Gly Pro His Pro Gly Asn Thr Ile Tyr
130 135 140Ala Ala Ser Lys His Ala Leu Arg Gly Leu Ala Asp Ala Phe Arg Lys145 150 155 160Glu Glu Ala Asn Asn Gly Ile Arg Val Ser Thr Val Ser Pro Gly Pro
165 170 175Thr Asn Thr Pro Met Leu Gln Gly Leu Met Asp Ser Gln Gly Thr Asn
180 185 190Phe Arg Pro Glu Ile Tyr Ile Glu Pro Lys Glu Ile Ala Asn Ala Ile
195 200 205Arg Phe Val Ile Asp Ala Gly Glu Thr Thr Gln Ile Thr Asn Val Asp
210 215 220Val Arg Pro Arg Ile Glu Leu Ala Asp Arg Lys Asp225 230 235<210>315<211>1008<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(985)<223>RXN02654<400>315tattttcgga aatttataca gcaatcctcg aaatcctaat aaagatccct tatcgtggga 60gaggtacggt agttcgttcg aggacaacgt cgagaaaggc atg att tca ttg cta 115
Met Ile Ser Leu Leu
1 5aat gat cca cgt acg cta ttc ccg aaa gtc gat ccc cca aag caa agc 163Asn Asp Pro Arg Thr Leu Phe Pro Lys Val Asp Pro Pro Lys Gln Ser
10 15 20cag ccg gaa cca ggc cta gat ata aaa ctt tcc ccc caa gcc gat att 211Gln Pro Glu Pro Gly Leu Asp Ile Lys Leu Ser Pro Gln Ala Asp Ile
25 30 35ggt ctc tcc agc tat caa gga agt gga agg ctt aag ggc cgc aag gct 259Gly Leu Ser Ser Tyr Gln Gly Ser Gly Arg Leu Lys Gly Arg Lys Ala
40 45 50ctt att act ggt ggc gat tct ggg att gga gct gcc gta gca atc gct 307Leu Ile Thr Gly Gly Asp Ser Gly Ile Gly Ala Ala Val Ala Ile Ala
55 60 65tat gct cgc gag ggg gca gat gtt gcg atc gct tac ttg ccc gaa gaa 355Tyr Ala Arg Glu Gly Ala Asp Val Ala Ile Ala Tyr Leu Pro Glu Glu70 75 80 85caa gcc gat gct gac aga gtg ctc caa gca atc gag gaa aca ggt caa 403Gln Ala Asp Ala Asp Arg Val Leu Gln Ala Ile Glu Glu Thr Gly Gln
90 95 100aaa gct ttt tct ttc cct ggt gat ctc cgt gat cca gaa tac tgt cgc 451Lys Ala Phe Ser Phe Pro Gly Asp Leu Arg Asp Pro Glu Tyr Cys Arg
105 110 115tcg ctg gtc caa gag acg gtg aac gct tta ggt ggc cta gac atc ttg 499Ser Leu Val Gln Glu Thr Val Asn Ala Leu Gly Gly Leu Asp Ile Leu
120 125 130gtc aac aac gcg tca cgt cag gtg tgg gca cct ggt ttg acc gaa att 547Val Asn Asn Ala Ser Arg Gln Val Trp Ala Pro Gly Leu Thr Glu Ile
135 140 145acc gac gaa aac ttc gac cag act ttg cag gtt aac ctc tat ggt agt 595Thr Asp Glu Asn Phe Asp Gln Thr Leu Gln Val Asn Leu Tyr Gly Ser150 155 160 165ttt cgg gtt acc aaa gca gct ata cct cat ctg aag ccc gga tca tcg 643Phe Arg Val Thr Lys Ala Ala Ile Pro His Leu Lys Pro Gly Ser Ser
170 175 180ata atc ttt aca tcg tcc att cag gcg tac caa cct tcg gaa acc ctc 691Ile Ile Phe Thr Ser Ser Ile Gln Ala Tyr Gln Pro Ser Glu Thr Leu
185 190 195ttg gat tac gcc atg act aag gcg gca ttg aac aat ttg tca aag ggc 739Leu Asp Tyr Ala Met Thr Lys Ala Ala Leu Asn Asn Leu Ser Lys Gly
200 205 210ttg gca agt agt ctg ara ggc gat ggc att cgg gta aat tct gta gcc 787Leu Ala Ser Ser Leu Ile Gly Asp Gly Ile Arg Val Asn Ser Val Ala
215 220 225cca ggt cct ttc tgg acg ccg ttg caa ccc agc cat ggt cag cca caa 835Pro Gly Pro Phe Trp Thr Pro Leu Gln Pro Ser His Gly Gln Pro Gln230 235 240 245gag aaa ata gaa gga ttt ggc cag cac gct ccg att gga aga gcg ggt 883Glu Lys Ile Glu Gly Phe Gly Gln His Ala Pro Ile Gly Arg Ala Gly
250 255 260cac cct gtt gag ttg gca ggt gcg tac gtt ttt ctc gct tct gac gaa 931His Pro Val Glu Leu Ala Gly Ala Tyr Val Phe Leu Ala Ser Asp Glu
265 270 275gcc agc tat gtg gta gga gaa acc ctg gga gtc aca ggt ggg acg ccc 979Ala Ser Tyr Val Val Gly Glu Thr Leu Gly Val Thr Gly Gly Thr Pro
280 285 290acc cca tagtcggtac aagcggaatc act 1008Thr Pro
295<210>316<211>295<212>PRT<213>谷氨酸棒杆菌<400>316Met Ile Ser Leu Leu Asn Asp Pro Arg Thr Leu Phe Pro Lys Val Asp1 5 10 15Pro Pro Lys Gln Ser Gln Pro Glu Pro Gly Leu Asp Ile Lys Leu Ser
20 25 30Pro Gln Ala Asp Ile Gly Leu Ser Ser Tyr Gln Gly Ser Gly Arg Leu
35 40 45Lys Gly Arg Lys Ala Leu Ile Thr Gly Gly Asp Ser Gly Ile Gly Ala
50 55 60Ala Val Ala Ile Ala Tyr Ala Arg Glu Gly Ala Asp Val Ala Ile Ala65 70 75 80Tyr Leu Pro Glu Glu Gln Ala Asp Ala Asp Arg Val Leu Gln Ala Ile
85 90 95Glu Glu Thr Gly Gln Lys Ala Phe Ser Phe Pro Gly Asp Leu Arg Asp
100 105 110Pro Glu Tyr Cys Arg Ser Leu Val Gln Glu Thr Val Asn Ala Leu Gly
115 120 125Gly Leu Asp Ile Leu Val Asn Asn Ala Ser Arg Gln Val Trp Ala Pro
130 135 140Gly Leu Thr Glu Ile Thr Asp Glu Asn Phe Asp Gln Thr Leu Gln Val145 150 155 160Asn Leu Tyr Gly Ser Phe Arg Val Thr Lys Ala Ala Ile Pro His Leu
165 170 175Lys Pro Gly Ser Ser Ile Ile Phe Thr Ser Ser Ile Gln Ala Tyr Gln
180 185 190Pro Ser Glu Thr Leu Leu Asp Tyr Ala Met Thr Lys Ala Ala Leu Asn
195 200 205Asn Leu Ser Lys Gly Leu Ala Ser Ser Leu Ile Gly Asp Gly Ile Arg
210 215 220Val Asn Ser Val Ala Pro Gly Pro Phe Trp Thr Pro Leu Gln Pro Ser225 230 235 240His Gly Gln Pro Gln Glu Lys Ile Glu Gly Phe Gly Gln His Ala Pro
245 250 255Ile Gly Arg Ala Gly His Pro Val Glu Leu Ala Gly Ala Tyr Val Phe
260 265 270Leu Ala Ser Asp Glu Ala Ser Tyr Val Val Gly Glu Thr Leu Gly Val
275 280 285Thr Gly Gly Thr Pro Thr Pro
290 295<210>317<211>1008<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(985)<223>FRXA02654<400>317tattttcgga aatttataca gcaatcctcg aaatcctaat aaagatccct tatcgtggga 60gaggtacggt agttcgttcg aggacaacgt cgagaaaggc atg att tca ttg cta 115
Met Ile Ser Leu Leu
1 5aat gat cca cgt acg cta ttc ccg aaa gtc gat ccc cca aag caa agc 163Asn Asp Pro Arg Thr Leu Phe Pro Lys Val Asp Pro Pro Lys Gln Ser
10 15 20cag ccg gaa cca ggc cta gat ata aaa ctt tcc ccc caa gcc gat att 211Gln Pro Glu Pro Gly Leu Asp Ile Lys Leu Ser Pro Gln Ala Asp Ile
25 30 35ggt ctc tcc agc tat caa gga agt gga agg ctt aag ggc cgc aag gct 259Gly Leu Ser Ser Tyr Gln Gly Ser Gly Arg Leu Lys Gly Arg Lys Ala
40 45 50ctt att act ggt ggc gat tct ggg att gga gct gcc gta gca atc gct 307Leu Ile Thr Gly Gly Asp Ser Gly Ile Gly Ala Ala Val Ala Ile Ala
55 60 65tat gct cgc gag ggg gca gat gtt gcg atc gct tac ttg ccc gaa gaa 355Tyr Ala Arg Glu Gly Ala Asp Val Ala Ile Ala Tyr Leu Pro Glu Glu70 75 80 85caa gcc gat gct gac aga gtg ctc caa gca atc gag gaa aca ggt caa 403Gln Ala Asp Ala Asp Arg Val Leu Gln Ala Ile Glu Glu Thr Gly Gln
90 95 100aaa gct ttt tct ttc cct ggt gat ctc cgt gat cca gaa tac tgt cgc 451Lys Ala Phe Ser Phe Pro Gly Asp Leu Arg Asp Pro Glu Tyr Cys Arg
105 110 115tcg ctg gtc caa gag acg gtg aac gct tta ggt ggc cta gac atc ttg 499Ser Leu Val Gln Glu Thr Val Asn Ala Leu Gly Gly Leu Asp Ile Leu
120 125 130gtc aac aac gcg tca cgt cag gtg tgg gca cct ggt ttg acc gaa att 547Val Asn Asn Ala Ser Arg Gln Val Trp Ala Pro Gly Leu Thr Glu Ile
135 140 145acc gac gaa aac ttc gac cag act ttg cag gtt aac ctc tat ggt agt 595Thr Asp Glu Asn Phe Asp Gln Thr Leu Gln Val Asn Leu Tyr Gly Ser150 155 160 165ttt cgg gtt acc aaa gca gct ata cct cat ctg aag ccc gga tca tcg 643Phe Arg Val Thr Lys Ala Ala Ile Pro His Leu Lys Pro Gly Ser Ser
170 175 180ata atc ttt aca tcg tcc att cag gcg tac caa cct tcg gaa acc ctc 691Ile Ile Phe Thr Ser Ser Ile Gln Ala Tyr Gln Pro Ser Glu Thr Leu
185 190 195ttg gat tac gcc atg act aag gcg gca ttg aac aat ttg tca aag ggc 739Leu Asp Tyr Ala Met Thr Lys Ala Ala Leu Asn Asn Leu Ser Lys Gly
200 205 210ttg gca agt agt ctg ata ggc gat ggc att cgg gta aat tct gta gcc 787Leu Ala Ser Ser Leu Ile Gly Asp Gly Ile Arg Val Asn Ser Val Ala
215 220 225cca ggt cct ttc tgg acg ccg ttg caa ccc agc cat ggt cag cca caa 835Pro Gly Pro Phe Trp Thr Pro Leu Gln Pro Ser His Gly Gln Pro Gln230 235 240 245gag aaa ata gaa gga ttt ggc cag cac gct ccg att gga aga gcg ggt 883Glu Lys Ile Glu Gly Phe Gly Gln His Ala Pro Ile Gly Arg Ala Gly
250 255 260cac cct gtt gag ttg gca ggt gcg tac gtt ttt ctc gct tct gac gaa 931His Pro Val Glu Leu Ala Gly Ala Tyr Val Phe Leu Ala Ser Asp Glu
265 270 275gcc agc tat gtg gta gga gaa acc ctg gga gtc aca ggt ggg acg ccc 979Ala Ser Tyr Val Val Gly Glu Thr Leu Gly Val Thr Gly Gly Thr Pro
280 285 290acc cca tagtcggtac aagcggaatc act 1008Thr Pro
295<210>318<211>295<212>PRT<213>谷氨酸棒杆菌<400>318Met Ile Ser Leu Leu Asn Asp Pro Arg Thr Leu Phe Pro Lys Val Asp1 5 10 15Pro Pro Lys Gln Ser Gln Pro Glu Pro Gly Leu Asp Ile Lys Leu Ser
20 25 30Pro Gln Ala Asp Ile Gly Leu Ser Ser Tyr Gln Gly Ser Gly Arg Leu
35 40 45Lys Gly Arg Lys Ala Leu Ile Thr Gly Gly Asp Ser Gly Ile Gly Ala
50 55 60Ala Val Ala Ile Ala Tyr Ala Arg Glu Gly Ala Asp Val Ala Ile Ala65 70 75 80Tyr Leu Pro Glu Glu Gln Ala Asp Ala Asp Arg Val Leu Gln Ala Ile
85 90 95Glu Glu Thr Gly Gln Lys Ala Phe Ser Phe Pro Gly Asp Leu Arg Asp
100 105 110Pro Glu Tyr Cys Arg Ser Leu Val Gln Glu Thr Val Asn Ala Leu Gly
115 120 125Gly Leu Asp Ile Leu Val Asn Asn Ala Ser Arg Gln Val Trp Ala Pro
130 135 140Gly Leu Thr Glu Ile Thr Asp Glu Asn Phe Asp Gln Thr Leu Gln Val145 150 155 160Asn Leu Tyr Gly Ser Phe Arg Val Thr Lys Ala Ala Ile Pro His Leu
165 170 175Lys Pro Gly Ser Ser Ile Ile Phe Thr Ser Ser Ile Gln Ala Tyr Gln
180 185 190Pro Ser Glu Thr Leu Leu Asp Tyr Ala Met Thr Lys Ala Ala Leu Asn
195 200 205Asn Leu Ser Lys Gly Leu Ala Ser Ser Leu Ile Gly Asp Gly Ile Arg
210 215 220Val Asn Ser Val Ala Pro Gly Pro Phe Trp Thr Pro Leu Gln Pro Ser225 230 235 240His Gly Gln Pro Gln Glu Lys Ile Glu Gly Phe Gly Gln His Ala Pro
245 250 255Ile Gly Arg Ala Gly His Pro Val Glu Leu Ala Gly Ala Tyr Val Phe
260 265 270Leu Ala Ser Asp Glu Ala Ser Tyr Val Val Gly Glu Thr Leu Gly Val
275 280 285Thr Gly Gly Thr Pro Thr Pro
290 295<210>319<211>1605<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1582)<223>RXN01049<400>319aagcacagca attgagcaat actcccatgc atgttttcgc gtgatcacgc tatatcctta 60aagaatattc tttattagtc agacctttaa aggaaacctt atg gga tca att cca 115
Met Gly Ser Ile Pro
1 5aca atg tcc atc cct ttt gat gac tca cgt gga cct tat gtc ctt gct 163Thr Met Ser Ile Pro Phe Asp Asp Ser Arg Gly Pro Tyr Val Leu Ala
10 15 20atg gat att ggt tcc act gca tca cga ggt gga ctt tat gat gct tcc 211Met Asp Ile Gly Ser Thr Ala Ser Arg Gly Gly Leu Tyr Asp Ala Ser
25 30 35ggc tgc cca atc aaa ggc acc aag cag cgc gaa tcc cat gaa ttc acc 259Gly Cys Pro Ile Lys Gly Thr Lys Gln Arg Glu Ser His Glu Phe Thr
40 45 50acc ggt gag ggc gtt tcc acc att gat gct gac cag gtg gtt tcg gag 307Thr Gly Glu Gly Val Ser Thr Ile Asp Ala Asp Gln Val Val Ser Glu
55 60 65atc acc tca gtt att aat ggc att ttg aac gcg gct gat cat cac aac 355Ile Thr Ser Val Ile Asn Gly Ile Leu Asn Ala Ala Asp His His Asn70 75 80 85atc aaa gat cag atc gcc gct gtc gcg cta gat tct ttt gca tcc tca 403Ile Lys Asp Gln Ile Ala Ala Val Ala Leu Asp Ser Phe Ala Ser Ser
90 95 100tta atc ttg gtc gat ggt gaa ggc aat gcg ctc acc ccg tgc att acc 451Leu Ile Leu Val Asp Gly Glu Gly Asn Ala Leu Thr Pro Cys Ile Thr
105 110 115tac gcg gat tct cgt tct gca cag tat gtg gag cag ctg cgc gcg gaa 499Tyr Ala Asp Ser Arg Ser Ala Gln Tyr Val Glu Gln Leu Arg Ala Glu
120 125 130atc gat gag aag gcc tac cac ggc cgc acc ggc gtc tgc ttg cac acc 547Ile Asp Glu Lys Ala Tyr His Gly Arg Thr Gly Val Cys Leu His Thr
135 140 145tcc tac cac cca tcg cgc ttg ctg tgg ctg aaa act gag ttc gag aaa 595Ser Tyr His Pro Ser Arg Leu Leu Trp Leu Lys Thr Glu Phe Glu Lys150 155 160 165gag ttc aac aaa gcc aag tat gtg atg acc atc ggt gag tac gtc tac 643Glu Phe Asn Lys Ala Lys Tyr Val Met Thr Ile Gly Glu Tyr Val Tyr
170 175 180ttc aaa ctt gca ggc atc acc gga atg gct act tcg att gcc gcg tgg 691Phe Lys Leu Ala Gly Ile Thr Gly Met Ala Thr Ser Ile Ala Ala Trp
185 190 195agt ggc att ttg gac gcc cat acc ggc gaa ctt gat ctg act atc ttg 739Ser Gly Ile Leu Asp Ala His Thr Gly Glu Leu Asp Leu Thr Ile Leu
200 205 210gag cac atc ggt gtt gat ccg gct ctg ttc ggt gag atc aga aac cct 787Glu His Ile Gly Val Asp Pro Ala Leu Phe Gly Glu Ile Arg Asn Pro
215 220 225gat gaa cca gcc acc gat gcc aaa gtt gtc gac aaa aag tgg aag cac 835Asp Glu Pro Ala Thr Asp Ala Lys Val Val Asp Lys Lys Trp Lys His230 235 240 245ctg gaa gaa atc cct tgg ttc cat gcc att cca gac ggc tgg cct tcc 883Leu Glu Glu Ile Pro Trp Phe His Ala Ile Pro Asp Gly Trp Pro Ser
250 255 260aac att ggc cca ggc gcc gtg gat tct aaa acc gtc gca gtc gcc gcc 931Asn Ile Gly Pro Gly Ala Val Asp Ser Lys Thr Val Ala Val Ala Ala
265 270 275gct aca tcc ggc gcc atg cgc gtg atc ctt ccg agc gtt ccc gaa cag 979Ala Thr Ser Gly Ala Met Arg Val Ile Leu Pro Ser Val Pro Glu Gln
280 285 290atc ccc tct ggc ctg tgg tgt tac cgc gtt tcc cgc gac cag tgc atc 1027Ile Pro Ser Gly Leu Trp Cys Tyr Arg Val Ser Arg Asp Gln Cys Ile
295 300 305gtt ggt ggc gca ctc aac gac gtc gga cgc gcc gtc acc tgg ctg gaa 1075Val Gly Gly Ala Leu Asn Asp Val Gly Arg Ala Val Thr Trp Leu Glu310 315 320 325cgc acc att atc aag cct gaa aac ctc gac gaa gtg ctg atc cgc gaa 1123Arg Thr Ile Ile Lys Pro Glu Asn Leu Asp Glu Val Leu Ile Arg Glu
330 335 340ccc ctc gaa ggc acc cca gct gtc ctg ccg ttc ttc tcc ggg gaa cgc 1171Pro Leu Glu Gly Thr Pro Ala Val Leu Pro Phe Phe Ser Gly Glu Arg
345 350 355tcc atc ggc tgg gca gcc tca gcg cag gcc acg atc acc aac att cag 1219Ser Ile Gly Trp Ala Ala Ser Ala Gln Ala Thr Ile Thr Asn Ile Gln
360 365 370gaa caa acc ggc cct gaa cac ttg tgg cgc ggc gtt ttc gaa gcc ctc 1267Glu Gln Thr Gly Pro Glu His Leu Trp Arg Gly Val Phe Glu Ala Leu
375 380 385gca ctc tcc tac cag cgc gtt tgg gaa cac atg ggg aaa gcc ggc gca 1315Ala Leu Ser Tyr Gln Arg Val Trp Glu His Met Gly Lys Ala Gly Ala390 395 400 405gcc cct gaa cgg gtc atc gca tca gga cga gtc tcc acc gac cac cca 1363Ala Pro Glu Arg Val Ile Ala Ser Gly Arg Val Ser Thr Asp His Pro
410 415 420gaa ttc ctc gcg atg ctt tcc gac gcc ctc gac acc cca gtc atc cct 1411Glu Phe Leu Ala Met Leu Ser Asp Ala Leu Asp Thr Pro Val Ile Pro
425 430 435ctg gaa atg aag cgc gcc acc ctc cgc ggc acc gca ctt atc gtc ctt 1459Leu Glu Met Lys Arg Ala Thr Leu Arg Gly Thr Ala Leu Ile Val Leu
440 445 450gag cag ctc gaa cca ggc ggc acg cgc gcg acg cca cca ttc ggc acg 1507Glu Gln Leu Glu Pro Gly Gly Thr Arg Ala Thr Pro Pro Phe Gly Thr
455 460 465acg cat cag ccg cgc ttt gcg cac cat tac tcc aag gca aga gag ctt 1555Thr His Gln Pro Arg Phe Ala His His Tyr Ser Lys Ala Arg Glu Leu470 475 480 485ttc gac gcc ctc tac ctc aag ttg gtc tagcttttcg cagtggaacg 1602Phe Asp Ala Leu Tyr Leu Lys Leu Val
490cgc 1605<210>320<211>494<212>PRT<213>谷氨酸棒杆菌<400>320Met Gly Ser Ile Pro Thr Met Ser Ile Pro Phe Asp Asp Ser Arg Gly1 5 10 15Pro Tyr Val Leu Ala Met Asp Ile Gly Ser Thr Ala Ser Arg Gly Gly
20 25 30Leu Tyr Asp Ala Ser Gly Cys Pro Ile Lys Gly Thr Lys Gln Arg Glu
35 40 45Ser His Glu Phe Thr Thr Gly Glu Gly Val Ser Thr Ile Asp Ala Asp
50 55 60Gln Val Val Ser Glu Ile Thr Ser Val Ile Asn Gly Ile Leu Asn Ala65 70 75 80Ala Asp His His Asn Ile Lys Asp Gln Ile Ala Ala Val Ala Leu Asp
85 90 95Ser Phe Ala Ser Ser Leu Ile Leu Val Asp Gly Glu Gly Asn Ala Leu
100 105 110Thr Pro Cys Ile Thr Tyr Ala Asp Ser Arg Ser Ala Gln Tyr Val Glu
115 120 125Gln Leu Arg Ala Glu Ile Asp Glu Lys Ala Tyr His Gly Arg Thr Gly
130 135 140Val Cys Leu His Thr Ser Tyr His Pro Ser Arg Leu Leu Trp Leu Lys145 150 155 160Thr Glu Phe Glu Lys Glu Phe Asn Lys Ala Lys Tyr Val Met Thr Ile
165 170 175Gly Glu Tyr Val Tyr Phe Lys Leu Ala Gly Ile Thr Gly Met Ala Thr
180 185 190Ser Ile Ala Ala Trp Ser Gly Ile Leu Asp Ala His Thr Gly Glu Leu
195 200 205Asp Leu Thr Ile Leu Glu His Ile Gly Val Asp Pro Ala Leu Phe Gly
210 215 220Glu Ile Arg Asn Pro Asp Glu Pro Ala Thr Asp Ala Lys Val Val Asp225 230 235 240Lys Lys Trp Lys His Leu Glu Glu Ile Pro Trp Phe His Ala Ile Pro
245 250 255Asp Gly Trp Pro Ser Asn Ile Gly Pro Gly Ala Val Asp Ser Lys Thr
260 265 270Val Ala Val Ala Ala Ala Thr Ser Gly Ala Met Arg Val Ile Leu Pro
275 280 285Ser Val Pro Glu Gln Ile Pro Ser Gly Leu Trp Cys Tyr Arg Val Ser
290 295 300Arg Asp Gln Cys Ile Val Gly Gly Ala Leu Asn Asp Val Gly Arg Ala305 310 315 320Val Thr Trp Leu Glu Arg Thr Ile Ile Lys Pro Glu Asn Leu Asp Glu
325 330 335Val Leu Ile Arg Glu Pro Leu Glu Gly Thr Pro Ala Val Leu Pro Phe
340 345 350Phe Ser Gly Glu Arg Ser Ile Gly Trp Ala Ala Ser Ala Gln Ala Thr
355 360 365Ile Thr Asn Ile Gln Glu Gln Thr Gly Pro Glu His Leu Trp Arg Gly
370 375 380Val Phe Glu Ala Leu Ala Leu Ser Tyr Glu Arg Val Trp Glu His Met385 390 395 400Gly Lys Ala Gly Ala Ala Pro Glu Arg Val Ile Ala Ser Gly Arg Val
405 410 415Ser Thr Asp His Pro Glu Phe Leu Ala Met Leu Ser Asp Ala Leu Asp
420 425 430Thr Pro Val Ile Pro Leu Glu Met Lys Arg Ala Thr Leu Arg Gly Thr
435 440 445Ala Leu Ile Val Leu Glu Gln Leu Glu Pro Gly Gly Thr Arg Ala Thr
450 455 460Pro Pro Phe Gly Thr Thr His Gln Pro Arg Phe Ala His His Tyr Ser465 470 475 480Lys Ala Arg Glu Leu Phe Asp Ala Leu Tyr Leu Lys Leu Val
485 490<210>321<211>1134<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1111)<223>FRXA01049<400>32lcacagtatgt ggagcagctg cgcgcggaaa tcgatgagaa ggcctaccac ggccgcaccg 60gcgtctgctt gcacacctcc taccacccat cgcgcttgct gtg gtg aaa act gag 115
Val Val Lys Thr Glu
1 5ttc gag aaa gag ttc aac aaa gcc aag tat gtg atg acc atc ggt gag 163Phe Glu Lys Glu Phe Asn Lys Ala Lys Tyr Val Met Thr Ile Gly Glu
10 15 20tac gtc tac ttc aaa ctt gca ggc atc acc gga atg gct act tcg att 211Tyr Val Tyr Phe Lys Leu Ala Gly Ile Thr Gly Met Ala Thr Ser Ile
25 30 35gcc gcg tgg agt ggc att ttg gac gcc cat acc ggc gaa ctt gat ctg 259Ala Ala Trp Ser Gly Ile Leu Asp Ala His Thr Gly Glu Leu Asp Leu
40 45 50act atc ttg gag cac atc ggt gtt gat ccg gct ctg ttc ggt gag atc 307Thr Ile Leu Glu His Ile Gly Val Asp Pro Ala Leu Phe Gly Glu Ile
55 60 65aga aac cct gat gaa cca gcc acc gat gcc aaa gtt gtc gac aaa aag 355Arg Asn Pro Asp Glu Pro Ala Thr Asp Ala Lys Val Val Asp Lys Lys70 75 80 85tgg aag cac ctg gaa gaa atc cct tgg ttc cat gcc att cca gac ggc 403Trp Lys His Leu Glu Glu Ile Pro Trp Phe His Ala Ile Pro Asp Gly
90 95 100tgg cct tcc aac att ggc cca ggc gcc gtg gat tct aaa acc gtc gca 451Trp Pro Ser Asn Ile Gly Pro Gly Ala Val Asp Ser Lys Thr Val Ala
105 110 115gtc gcc gcc gct aca tcc ggc gcc atg cgc gtg atc ctt ccg agc gtt 499Val Ala Ala Ala Thr Ser Gly Ala Met Arg Val Ile Leu Pro Ser Val
120 125 130ccc gaa cag atc ccc tct ggc ctg tgg tgt tac cgc gtt tcc cgc gac 547Pro Glu Gln Ile Pro Ser Gly Leu Trp Cys Tyr Arg Val Ser Arg Asp
135 140 145cag tgc atc gtt ggt ggc gca ctc aac gac gtc gga cgc gcc gtc acc 595Gln Cys Ile Val Gly Gly Ala Leu Asn Asp Val Gly Arg Ala Val Thr150 155 160 165tgg ctg gaa cgc acc att atc aag cct gaa aac ctc gac gaa gtg ctg 643Trp Leu Glu Arg Thr Ile Ile Lys Pro Glu Asn Leu Asp Glu Val Leu
170 175 180atc cgc gaa ccc ctc gaa ggc acc cca gct gtc ctg ccg ttc ttc tcc 691Ile Arg Glu Pro Leu Glu Gly Thr Pro Ala Val Leu Pro Phe Phe Ser
185 190 195ggg gaa cgc tcc atc ggc tgg gca gcc tca gcg cag gcc acg atc acc 739Gly Glu Arg Ser Ile Gly Trp Ala Ala Ser Ala Gln Ala Thr Ile Thr
200 205 210aac att cag gaa caa acc ggc cct gaa cac ttg tgg cgc ggc gtt ttc 787Asn Ile Gln Glu Gln Thr Gly Pro Glu His Leu Trp Arg Gly Val Phe
215 220 225gaa gcc ctc gca ctc tcc tac cag cgc gtt tgg gaa cac atg ggg aaa 835Glu Ala Leu Ala Leu Ser Tyr Gln Arg Val Trp Glu His Met Gly Lys230 235 240 245gcc ggc gca gcc cct gaa cgg gtc atc gca tca gga cga gtc tcc acc 883Ala Gly Ala Ala Pro Glu Arg Val Ile Ala Ser Gly Arg Val Ser Thr
250 255 260gac cac cca gaa ttc ctc gcg atg ctt tcc gac gcc ctc gac acc cca 931Asp His Pro Glu Phe Leu Ala Met Leu Ser Asp Ala Leu Asp Thr Pro
265 270 275gtc atc cct ctg gaa atg aag cgc gcc acc ctc cgc ggc acc gca ctt 979Val Ile Pro Leu Glu Met Lys Arg Ala Thr Leu Arg Gly Thr Ala Leu
280 285 290atc gtc ctt gag cag ctc gaa cca ggc ggc acg cgc gcg acg cca cca 1027Ile Val Leu Glu Gln Leu Glu Pro Gly Gly Thr Arg Ala Thr Pro Pro
295 300 305ttc ggc acg acg cat cag ccg cgc ttt gcg cac cat tac tcc aag gca 1075Phe Gly Thr Thr His Gln Pro Arg Phe Ala His His Tyr Ser Lys Ala310 315 320 325aga gag ctt ttc gac gcc ctc tac ctc aag ttg gtc tagcttttcg 1121Arg Glu Leu Phe Asp Ala Leu Tyr Leu Lys Leu Val
330 335cagtggaacg cgc 1134<210>322<211>337<212>PRT<213>谷氨酸棒杆菌<400>322Val Val Lys Thr Glu Phe Glu Lys Glu Phe Asn Lys Ala Lys Tyr Val1 5 10 15Met Thr Ile Gly Glu Tyr Val Tyr Phe Lys Leu Ala Gly Ile Thr Gly
20 25 30Met Ala Thr Ser Ile Ala Ala Trp Ser Gly Ile Leu Asp Ala His Thr
35 40 45Gly Glu Leu Asp Leu Thr Ile Leu Glu His Ile Gly Val Asp Pro Ala
50 55 60Leu Phe Gly Glu Ile Arg Asn Pro Asp Glu Pro Ala Thr Asp Ala Lys65 70 75 80Val Val Asp Lys Lys Trp Lys His Leu Glu Glu Ile Pro Trp Phe His
85 90 95Ala Ile Pro Asp Gly Trp Pro Ser Asn Ile Gly Pro Gly Ala Val Asp
100 105 110Ser Lys Thr Val Ala Val Ala Ala Ala Thr Ser Gly Ala Met Arg Val
115 120 125Ile Leu Pro Ser Val Pro Glu Gln Ile Pro Ser Gly Leu Trp Cys Tyr
130 135 140Arg Val Ser Arg Asp Gln Cys Ile Val Gly Gly Ala Leu Asn Asp Val145 150 155 160Gly Arg Ala Val Thr Trp Leu Glu Arg Thr Ile Ile Lys Pro Glu Asn
165 170 175Leu Asp Glu Val Leu Ile Arg Glu Pro Leu Glu Gly Thr Pro Ala Val
180 185 190Leu Pro Phe Phe Ser Gly Glu Arg Ser Ile Gly Trp Ala Ala Ser Ala
195 200 205Gln Ala Thr Ile Thr Asn Ile Gln Glu Gln Thr Gly Pro Glu His Leu
210 215 220Trp Arg Gly Val Phe Glu Ala Leu Ala Leu Ser Tyr Gln Arg Val Trp225 230 235 240Glu His Met Gly Lys Ala Gly Ala Ala Pro Glu Arg Val Ile Ala Ser
245 250 255Gly Arg Val Ser Thr Asp His Pro Glu Phe Leu Ala Met Leu Ser Asp
260 265 270Ala Leu Asp Thr Pro Val Ile Pro Leu Glu Met Lys Arg Ala Thr Leu
275 280 285Arg Gly Thr Ala Leu Ile Val Leu Glu Gln Leu Glu Pro Gly Gly Thr
290 295 300Arg Ala Thr Pro Pro Phe Gly Thr Thr His Gln Pro Arg Phe Ala His305 310 315 320His Tyr Ser Lys Ala Arg Glu Leu Phe Asp Ala Leu Tyr Leu Lys Leu
325 330 335Val<210>323<211>597<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(574)<223>FRXA01050<400>323aagcacagca attgagcaat actcccatgc atgttttcgc gtgatcacgc tatatcctta 60aagaatattc tttattagtc agacctttaa aggaaacctt atg gga tca att cca 115
Met Gly Ser Ile Pro
1 5aca atg tcc atc cct ttt gat gac tca cgt gga cct tat gtc ctt gct 163Thr Met Ser Ile Pro Phe Asp Asp Ser Arg Gly Pro Tyr Val Leu Ala
10 15 20atg gat att ggt tcc act gca tca cga ggt gga ctt tat gat gct tcc 211Met Asp Ile Gly Ser Thr Ala Ser Arg Gly Gly Leu Tyr Asp Ala Ser
25 30 35ggc tgc cca atc aaa ggc acc aag cag cgc gaa tcc cat gaa ttc acc 259Gly Cys Pro Ile Lys Gly Thr Lys Gln Arg Glu Ser His Glu Phe Thr
40 45 50acc ggt gag ggc att tcc acc att gat gct gac cag gtg gtt tcg gag 307Thr Gly Glu Gly Val Ser Thr Ile Asp Ala Asp Gln Val Val Ser Glu
55 60 65atc acc tca gtt att aat ggc att ttg aac gcg gct gat cat cac aac 355Ile Thr Ser Val Ile Asn Gly Ile Leu Asn Ala Ala Asp His His Asn70 75 80 85atc aaa gat cag atc gcc gct gtc gcg cta gat tct ttt gca tcc tca 403Ile Lys Asp Gln Ile Ala Ala Val Ala Leu Asp Ser Phe Ala Ser Ser
90 95 100tta atc ttg gtc gat ggt gaa ggc aat gcg ctc acc ccg tgc att acc 451Leu Ile Leu Val Asp Gly Glu Gly Asn Ala Leu Thr Pro Cys Ile Thr
105 110 115tac gcg gat tct cgt tct gca cag tat gtg gag cag ctg cgc gcg gaa 499Tyr Ala Asp Ser Arg Ser Ala Gln Tyr Val Glu Gln Leu Arg Ala Glu
120 125 130atc gat gag aag gcc tac cac ggc cgc acc ggc gtc tgc ttg cac acc 547Ile Asp Glu Lys Ala Tyr His Gly Arg Thr Gly Val Cys Leu His Thr
135 140 145tcc tac cac cca tcg cgc ttg ctg tgg tgaaaactga gttcgagaaa 594Ser Tyr His Pro Ser Arg Leu Leu Trp150 155gag 597<210>324<211>158<212>PRT<213>谷氨酸棒杆菌<400>324Met Gly Ser Ile Pro Thr Met Ser Ile Pro Phe Asp Asp Ser Arg Gly1 5 10 15Pro Tyr Val Leu Ala Met Asp Ile Gly Ser Thr Ala Ser Arg Gly Gly
20 25 30Leu Tyr Asp Ala Ser Gly Cys Pro Ile Lys Gly Thr Lys Gln Arg Glu
35 40 45Ser His Glu Phe Thr Thr Gly Glu Gly Val Ser Thr Ile Asp Ala Asp
50 55 60Gln Val Val Ser Glu Ile Thr Ser Val Ile Asn Gly Ile Leu Asn Ala65 70 75 80Ala Asp His His Asn Ile Lys Asp Gln Ile Ala Ala Val Ala Leu Asp
85 90 95Ser Phe Ala Ser Ser Leu Ile Leu Val Asp Gly Glu Gly Asn Ala Leu
100 105 110Thr Pro Cys Ile Thr Tyr Ala Asp Ser Arg Ser Ala Gln Tyr Val Glu
115 120 125Gln Leu Arg Ala Glu Ile Asp Glu Lys Ala Tyr His Gly Arg Thr Gly
130 135 140Val Cys Leu His Thr Ser Tyr His Pro Ser Arg Leu Leu Trp145 150 155<210>325<211>1065<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1042)<223>RXA00202<400>325ctggcagcag attgtcatcg gttgtgtcat cgcgcttgcg gtgggcttcg atgtcatccg 60aaacaaaacc tctaagtaat tcctgaaagg aaattttcac atg tac gct cgt aaa 115
Met Tyr Ala Arg Lys
1 5ctt att gct ctg tcc gct tct gtc gtt ttg gct ttc agc ttg tct gct 163Leu Ile Ala Leu Ser Ala Ser Val Val Leu Ala Phe Ser Leu Ser Ala
10 15 20tgc aac cgt gaa tct tct ggc acc agc gca gac ggc ggt tct gcg gat 211Cys Asn Arg Glu Ser Ser Gly Thr Ser Ala Asp Gly Gly Ser Ala Asp
25 30 35ggg tcg atc acc ttg gct ctg tct acc cag acc aac ccg ttc ttt gtg 259Gly Ser Ile Thr Leu Ala Leu Ser Thr Gln Thr Asn Pro Phe Phe Val
40 45 50cag ctt cgt gat ggt gcc cag gaa aag gct gat gaa ttg ggc gtg acc 307Gln Leu Arg Asp Gly Ala Gln Glu Lys Ala Asp Glu Leu Gly Val Thr
55 60 65ctc aat gtt cag gat gct tcc gat gac gct gca acg cag gcc aac cag 355Leu Asn Val Gln Asp Ala Ser Asp Asp Ala Ala Thr Gln Ala Asn Gln70 75 80 85ctc aac aac gct gtc acc acc ggt gct ggc gtg gtg att gtc aac cca 403Leu Asn Asn Ala Val Thr Thr Gly Ala Gly Val Val Ile Val Asn Pro
90 95 100act gat tct gat gct gtg gtg ccg tcg gtg gaa gct ctc aac cag gct 451Thr Asp Ser Asp Ala Val Val Pro Ser Val Glu Ala Leu Asn Gln Ala
105 110 115gac att cct gtt gtg gct gtc gac cgt tcc tcc aat ggt ggc gag gtg 499Asp Ile Pro Val Val Ala Val Asp Arg Ser Ser Asn Gly Gly Glu Val
120 125 130gcg tcc ttc gtg gca tct gac aac gtt gct ggc ggc gcg cag gct gct 547Ala Ser Phe Val Ala Ser Asp Asn Val Ala Gly Gly Ala Gln Ala Ala
135 140 145gca gcc ctg gca gag gcg atc ggt ggc gaa ggt gaa atc ctc atg ctg 595Ala Ala Leu Ala Glu Ala Ile Gly Gly Glu Gly Glu Ile Leu Met Leu150 155 160 165caa ggc att gcg gga tcc tct gca tca cgt gat cgt gga cag gga ttt 643Gln Gly Ile Ala Gly Ser Ser Ala Ser Arg Asp Arg Gly Gln Gly Phe
170 175 180gaa gag gag atc gct aag cat gag ggc att tcc att gtg gct aag cag 691Glu Glu Glu Ile Ala Lys His Glu Gly Ile Ser Ile Val Ala Lys Gln
185 190 195acc gcc aac ttt gac cgc ggt gag ggc ctg gac gtg gca act aac ctg 739Thr Ala Asn Phe Asp Arg Gly Glu Gly Leu Asp Val Ala Thr Asn Leu
200 205 210ctg cag gca cac ccc aat gtg aag gcg atc ttc gcg gaa aac gat gag 787Leu Gln Ala His Pro Asn Val Lys Ala Ile Phe Ala Glu Asn Asp Glu
215 220 225atg gcg ttg ggc gca atc gaa gcc ctg ggt gct cgt gct ggt gaa gat 835Met Ala Leu Gly Ala Ile Glu Ala Leu Gly Ala Arg Ala Gly Glu Asp230 235 240 245gtc atc gtt gtc ggt ttc gat ggc acc aat gat ggt ctg gca gcg gtt 883Val Ile Val Val Gly Phe Asp Gly Thr Asn Asp Gly Leu Ala Ala Val
250 255 260gaa gat gga cgc atg ttg gcc acc gtt gct cag cag cca gaa gag ctg 931Glu Asp Gly Arg Met Leu Ala Thr Val Ala Gln Gln Pro Glu Glu Leu
265 270 275gga gca aag gct gtg gaa gaa gca gct aag ctc ctg cgc ggt gag gac 979Gly Ala Lys Ala Val Glu Glu Ala Ala Lys Leu Leu Arg Gly Glu Asp
280 285 290gct gaa aca gag gta cca gtt gag gtt gtc act gtg aag ctc gac aac 1027Ala Glu Thr Glu Val Pro Val Glu Val Val Thr Val Lys Leu Asp Asn
295 300 305gtc gcg gac ttc aag tagtcggcga tgaaaaagtc cgt 1065Val Ala Asp Phe Lys310<210>326<211>314<212>PRT<213>谷氨酸棒杆菌<400>326Met Tyr Ala Arg Lys Leu Ile Ala Leu Ser Ala Ser Val Val Leu Ala1 5 10 15Phe Ser Leu Ser Ala Cys Asn Arg Glu Ser Ser Gly Thr Ser Ala Asp
20 25 30Gly Gly Ser Ala Asp Gly Ser Ile Thr Leu Ala Leu Ser Thr Gln Thr
35 40 45Asn Pro Phe Phe Val Gln Leu Arg Asp Gly Ala Gln Glu Lys Ala Asp
50 55 60Glu Leu Gly Val Thr Leu Asn Val Gln Asp Ala Ser Asp Asp Ala Ala65 70 75 80Thr Gln Ala Asn Gln Leu Asn Asn Ala Val Thr Thr Gly Ala Gly Val
85 90 95Val Ile Val Asn Pro Thr Asp Ser Asp Ala Val Val Pro Ser Val Glu
100 105 110Ala Leu Asn Gln Ala Asp Ile Pro Val Val Ala Val Asp Arg Ser Ser
115 120 125Asn Gly Gly Glu Val Ala Ser Phe Val Ala Ser Asp Asn Val Ala Gly
130 135 140Gly Ala Gln Ala Ala Ala Ala Leu Ala Glu Ala Ile Gly Gly Glu Gly145 150 155 160Glu Ile Leu Met Leu Gln Gly Ile Ala Gly Ser Ser Ala Ser Arg Asp
165 170 175Arg Gly Gln Gly Phe Glu Glu Glu Ile Ala Lys His Glu Gly Ile Ser
180 185 190Ile Val Ala Lys Gln Thr Ala Asn Phe Asp Arg Gly Glu Gly Leu Asp
195 200 205Val Ala Thr Asn Leu Leu Gln Ala His Pro Asn Val Lys Ala Ile Phe
210 215 220Ala Glu Asn Asp Glu Met Ala Leu Gly Ala Ile Glu Ala Leu Gly Ala225 230 235 240Arg Ala Gly Glu Asp Val Ile Val Val Gly Phe Asp Gly Thr Asn Asp
245 250 255Gly Leu Ala Ala Val Glu Asp Gly Arg Met Leu Ala Thr Val Ala Gln
260 265 270Gln Pro Glu Glu Leu Gly Ala Lys Ala Val Glu Glu Ala Ala Lys Leu
275 280 285Leu Arg Gly Glu Asp Ala Glu Thr Glu Val Pro Val Glu Val Val Thr
290 295 300Val Lys Leu Asp Asn Val Ala Asp Phe Lys305 310<210>327<211>1077<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1054)<223>RXN00872<400>327gaaatgtatt gctttgtcag gacaatgtgt tattgtcatg acatgcgatc gtgagggtcg 60ccacattcca tcaaaaatga gtgaagggtt gcatcgccac atg act aac ttg acg 115
Met Thr Asn Leu Thr
1 5agc act cac gaa gtc cta gct atc ggc cgc ttg ggc gta gat att tac 163Ser Thr His Glu Val Leu Ala Ile Gly Arg Leu Gly Val Asp Ile Tyr
10 15 20cca ctt caa agt gga gta gga ctg gcc gat gtt caa tct ttc ggc aag 211Pro Leu Gln Ser Gly Val Gly Leu Ala Asp Val Gln Ser Phe Gly Lys
25 30 35tac ctc ggc gga agc gca gca aac gtt tct gtt gca gcc gcc cgc cat 259Tyr Leu Gly Gly Ser Ala Ala Asn Val Ser Val Ala Ala Ala Arg His
40 45 50gga cac aat tcc gca ctg ctg tcc cgt gtg gga aat gat cct ttc ggc 307Gly His Asn Ser Ala Leu Leu Ser Arg Val Gly Asn Asp Pro Phe Gly
55 60 65gag tac ctg ctt gct gag ctg gag cgt ttg ggc gtg gac aac cag tac 355Glu Tyr Leu Leu Ala Glu Leu Glu Arg Leu Gly Val Asp Asn Gln Tyr70 75 80 85gtt gcc acc gat cag act ttt aag acc cca gtg acc ttc tgt gaa att 403Val Ala Thr Asp Gln Thr Phe Lys Thr Pro Val Thr Phe Cys Glu Ile
90 95 100ttc cca ccg gat gat ttc cca ctg tac ttc tac cgc gaa cca aag gct 451Phe Pro Pro Asp Asp Phe Pro Leu Tyr Phe Tyr Arg Glu Pro Lys Ala
105 110 115ccg gat ctc aat att gaa tcc gca gac gtc agc ctg gac gat gtg cgc 499Pro Asp Leu Asn Ile Glu Ser Ala Asp Val Ser Leu Asp Asp Val Arg
120 125 130gaa gcc gat att ttg tgg ttc aca ctc act ggt ttc agt gaa gag cca 547Glu Ala Asp Ile Leu Trp Phe Thr Leu Thr Gly Phe Ser Glu Glu Pro
135 140 145agc cgc ggc aca cac cgc gag atc ttg act act cgt gcg aac cgt cgc 595Ser Arg Gly Thr His Arg Glu Ile Leu Thr Thr Arg Ala Asn Arg Arg150 155 160 165cac acc atc ttt gat ctg gac tac cga cca atg ttc tgg gaa tcc cca 643His Thr Ile Phe Asp Leu Asp Tyr Arg Pro Met Phe Trp Glu Ser Pro
170 175 180gaa gag gcc acc aag cag gcg gaa tgg gcg ttg cag cat tcc acg gtg 691Glu Glu Ala Thr Lys Gln Ala Glu Trp Ala Leu Gln His Ser Thr Val
185 190 195gcg gtt ggc aac aag gaa gaa tgc gaa atc gca gtg ggc gag acc gag 739Ala Val Gly Asn Lys Glu Glu Cys Glu Ile Ala Val Gly Glu Thr Glu
200 205 210cca gag cgc gcg ggc cga gca ctg ttg gaa cgc ggt gtg gag ttg gcc 787Pro Glu Arg Ala Gly Arg Ala Leu Leu Glu Arg Gly Val Glu Leu Ala
215 220 225atc gtc aag cag gga cct aag ggt gtc atg gcg atg acc aag gac gaa 835Ile Val Lys Gln Gly Pro Lys Gly Val Met Ala Met Thr Lys Asp Glu230 235 240 245acc gta gaa gtt cct ccg ttc ttc gtc gat gtc atc aac ggt ctt ggt 883Thr Val Glu Val Pro Pro Phe Phe Val Asp Val Ile Asn Gly Leu Gly
250 255 260gcc ggc gat gca ttc ggc ggc gcg ctg tgc cac ggt ctg ctc tct gaa 931Ala Gly Asp Ala Phe Gly Gly Ala Leu Cys His Gly Leu Leu Ser Glu
265 270 275tgg ccg ttg gaa aag gtt ctc cgt ttt gcc aac acc gcg ggt gcg ctt 979Trp Pro Leu Glu Lys Val Leu Arg Phe Ala Asn Thr Ala Gly Ala Leu
280 285 290gtg gcg tcc cgt ctt gaa tgc tcc acc gca atg cct act acc gat gag 1027Val Ala Ser Arg Leu Glu Cys Ser Thr Ala Met Pro Thr Thr Asp Glu
295 300 305gtg gaa gcc tcc ctc aac cag aaa gtc tgatatgact cctccgatta 1074Val Glu Ala Ser Leu Asn Gln Lys Val310 315tct 1077<210>328<211>318<212>PRT<213>谷氨酸棒杆菌<400>328Met Thr Asn Leu Thr Ser Thr His Glu Val Leu Ala Ile Gly Arg Leu1 5 10 15Gly Val Asp Ile Tyr Pro Leu Gln Ser Gly Val Gly Leu Ala Asp Val
20 25 30Gln Ser Phe Gly Lys Tyr Leu Gly Gly Ser Ala Ala Asn Val Ser Val
35 40 45Ala Ala Ala Arg His Gly His Asn Ser Ala Leu Leu Ser Arg Val Gly
50 55 60Asn Asp Pro Phe Gly Glu Tyr Leu Leu Ala Glu Leu Glu Arg Leu Gly65 70 75 80Val Asp Asn Gln Tyr Val Ala Thr Asp Gln Thr Phe Lys Thr Pro Val
85 90 95Thr Phe Cys Glu Ile Phe Pro Pro Asp Asp Phe Pro Leu Tyr Phe Tyr
100 105 110Arg Glu Pro Lys Ala Pro Asp Leu Asn Ile Glu Ser Ala Asp Val Ser
115 120 125Leu Asp Asp Val Arg Glu Ala Asp Ile Leu Trp Phe Thr Leu Thr Gly
130 135 140Phe Ser Glu Glu Pro Ser Arg Gly Thr His Arg Glu Ile Leu Thr Thr145 150 155 160Arg Ala Asn Arg Arg His Thr Ile Phe Asp Leu Asp Tyr Arg Pro Met
165 170 175Phe Trp Glu Ser Pro Glu Glu Ala Thr Lys Gln Ala Glu Trp Ala Leu
180 185 190Gln His Ser Thr Val Ala Val Gly Asn Lys Glu Glu Cys Glu Ile Ala
195 200 205Val Gly Glu Thr Glu Pro Glu Arg Ala Gly Arg Ala Leu Leu Glu Arg
210 215 220Gly Val Glu Leu Ala Ile Val Lys Gln Gly Pro Lys Gly Val Met Ala225 230 235 240Met Thr Lys Asp Glu Thr Val Glu Val Pro Pro Phe Phe Val Asp Val
245 250 255Ile Asn Gly Leu Gly Ala Gly Asp Ala Phe Gly Gly Ala Leu Cys His
260 265 270Gly Leu Leu Ser Glu Trp Pro Leu Glu Lys Val Leu Arg Phe Ala Asn
275 280 285Thr Ala Gly Ala Leu Val Ala Ser Arg Leu Glu Cys Ser Thr Ala Met
290 295 300Pro Thr Thr Asp Glu Val Glu Ala Ser Leu Asn Gln Lys Val305 310 315<210>329<211>622<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(622)<223>FRXA00872<400>329gaaatgtatt gctttgtcag gacaatgtgt tattgtcatg acatgcgatc gtgagggtcg 60ccacattcca tcaaaaatga gtgaagggtt gcatcgccac atg act aac ttg acg 115
Met Thr Asn Leu Thr
1 5agc act cac gaa gtc cta gct atc ggc cgc ttg ggc gta gat att tac 163Ser Thr His Glu Val Leu Ala Ile Gly Arg Leu Gly Val Asp Ile Tyr
10 15 20cca ctt caa agt gga gta gga ctg gcc gat gtt caa tct ttc ggc aag 211Pro Leu Gln Ser Gly Val Gly Leu Ala Asp Val Gln Ser Phe Gly Lys
25 30 35tac ctc ggc gga agc gca gca aac gtt tct gtt gca gcc gcc cgc cat 259Tyr Leu Gly Gly Ser Ala Ala Asn Val Ser Val Ala Ala Ala Arg His
40 45 50gga cac aat tcc gca ctg ctg tcc cgt gtg gga aat gat cct ttc ggc 307Gly His Asn Ser Ala Leu Leu Ser Arg Val Gly Asn Asp Pro Phe Gly
55 60 65gag tac ctg ctt gct gag ctg gag cgt ttg ggc gtg gac aac cag tac 355Glu Tyr Leu Leu Ala Glu Leu Glu Arg Leu Gly Val Asp Asn Gln Tyr70 75 80 85gtt gcc acc gat cag act ttt aag acc cca gtg acc ttc tgt gaa att 403Val Ala Thr Asp Gln Thr Phe Lys Thr Pro Val Thr Phe Cys Glu Ile
90 95 100ttc cca ccg gat gat ttc cca ctg tac ttc tac cgc gaa cca aag gct 451Phe Pro Pro Asp Asp Phe Pro Leu Tyr Phe Tyr Arg Glu Pro Lys Ala
105 110 115ccg gat ctc aat att gaa tcc gca gac gtc agc ctg gac gat gtg cgc 499Pro Asp Leu Asn Ile Glu Ser Ala Asp Val Ser Leu Asp Asp Val Arg
120 125 130gaa gcc gat att ttg tgg ttc aca ctc act ggt ttc agt gaa gag cca 547Glu Ala Asp Ile Leu Trp Phe Thr Leu Thr Gly Phe Ser Glu Glu Pro
135 140 145agc cgc ggc aca cac cgc gag atc ttg act act cgt gcg aac cgt cgc 595Ser Arg Gly Thr His Arg Glu Ile Leu Thr Thr Arg Ala Asn Arg Arg150 155 160 165cac acc atc ttt gat ctg gac tac cga 622His Thr Ile Phe Asp Leu Asp Tyr Arg
170<210>330<211>174<212>PRT<213>谷氨酸棒杆菌<400>330Met Thr Asn Leu Thr Ser Thr His Glu Val Leu Ala Ile Gly Arg Leu1 5 10 15Gly Val Asp Ile Tyr Pro Leu Gln Ser Gly Val Gly Leu Ala Asp Val
20 25 30Gln Ser Phe Gly Lys Tyr Leu Gly Gly Ser Ala Ala Asn Val Ser Val
35 40 45Ala Ala Ala Arg His Gly His Asn Ser Ala Leu Leu Ser Arg Val Gly
50 55 60Asn Asp Pro Phe Gly Glu Tyr Leu Leu Ala Glu Leu Glu Arg Leu Gly65 70 75 80Val Asp Asn Gln Tyr Val Ala Thr Asp Gln Thr Phe Lys Thr Pro Val
85 90 95Thr Phe Cys Glu Ile Phe Pro Pro Asp Asp Phe Pro Leu Tyr Phe Tyr
100 105 110Arg Glu Pro Lys Ala Pro Asp Leu Asn Ile Glu Ser Ala Asp Val Ser
115 120 125Leu Asp Asp Val Arg Glu Ala Asp Ile Leu Trp Phe Thr Leu Thr Gly
130 135 140Phe Ser Glu Glu Pro Ser Arg Gly Thr His Arg Glu Ile Leu Thr Thr145 150 155 160Arg Ala Asn Arg Arg His Thr Ile Phe Asp Leu Asp Tyr Arg
165 170<210>331<211>1767<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1744)<223>RXN00799<400>331ttgtctaacc ccgtataggt gagaaatgtt ggacaagtgt ctgttttttg tgggggaaat 60ctgactacga tggtaagaaa taaggaaaga gattaccatt atg tct caa gag cgg 115
Met Ser Gln Glu Arg
1 5cct caa atc ggc tcc cgc ctc tct cgt gtc att gaa caa gac ggc cta 163Pro Gln Ile Gly Ser Arg Leu Ser Arg Val Ile Glu Gln Asp Gly Leu
10 15 20caa ttc cgc gat ctc gac ggc gac ggc gta ctt gca cct tat gaa gat 211Gln Phe Arg Asp Leu Asp Gly Asp Gly Val Leu Ala Pro Tyr Glu Asp
25 30 35tgg cgt cta acc cca gca gag cgt gcc gct gac ctg gtg aaa cgt atg 259Trp Arg Leu Thr Pro Ala Gln Arg Ala Ala Asp Leu Val Lys Arg Met
40 45 50aat gtg gaa gaa aaa gcg ggc ctg atg atc atc ggt tcg cac tac ccc 307Asn Val Gln Glu Lys Ala Gly Leu Met Ile Ile Gly Ser His Tyr Pro
55 60 65gga tac tcg cct ttg gcg ccg gag agt gaa ggc aaa gac gcg gaa aag 355Gly Tyr Ser Pro Leu Ala Pro Glu Ser Glu Gly Lys Asp Ala Glu Lys70 75 80 85tgc gag cct ttg ctg aac cct gtc gat atg tgg cgt gag gat aac ccg 403Cys Glu Pro Leu Leu Asn Pro Val Asp Met Trp Arg Glu Asp Asn Pro
90 95 100atc acg ggt gtt cct ttc acc gag cct gtg ctg gca act tct tcc act 451Ile Thr Gly Val Pro Phe Thr Glu Pro Val Leu Ala Thr Ser Ser Thr
105 110 115gaa aat gcc att aac ctg cgc aat cag cgt tac tta att gtt cgt gac 499Glu Asn Ala Ile Asn Leu Arg Asn Gln Arg Tyr Leu Ile Val Arg Asp
120 125 130aac ctg cca gct cgt ggg ctt gct act tgg acc aat gct gtt cag gaa 547Asn Leu Pro Ala Arg Gly Leu Ala Thr Trp Thr Asn Ala Val Gln Glu
135 140 145gtc gcg gag cga tcc cgt ttg ggt att cct gtt gcg ttt gcg tcg aat 595Val Ala Glu Arg Ser Arg Leu Gly Ile Pro Val Ala Phe Ala Ser Asn150 155 160 165cct cgt aac cac gtc gcg ctc gtt gcg cag ttc ggt gtg aac gag tcc 643Pro Arg Asn His Val Ala Leu Val Ala Gln Phe Gly Val Asn Glu Ser
170 175 180gcg ggt gtg ttc tct gag tgg cct ggc gag ctg ggt ctt gct gcg ctt 691Ala Gly Val Phe Ser Glu Trp Pro Gly Glu Leu Gly Leu Ala Ala Leu
185 190 195cgc gat gct gaa ctg atg gag act ttc ggt acc gag gct gct aaa gaa 739Arg Asp Ala Glu Leu Met Glu Thr Phe Gly Thr Glu Ala Ala Lys Glu
200 205 210tgg cgt gcc ggt ggt gtg cac aag ctg tac ggt tac atg gct gac ctc 787Trp Arg Ala Gly Gly Val His Lys Leu Tyr Gly Tyr Met Ala Asp Leu
215 220 225gct tct gag cct cgt tgg tcc cgc ttc aac ggt act ttt ggt gag gat 835Ala Ser Glu Pro Arg Trp Ser Arg Phe Asn Gly Thr Phe Gly Glu Asp230 235 240 245ccg gag ttg atc tct gat tac atc gct gct gtt gtg cgt ggt ttg cag 883Pro Glu Leu Ile Ser Asp Tyr Ile Ala Ala Val Val Arg Gly Leu Gln
250 255 260ggc cct gag ctg tcc aag aat tcc gtg tcg acc acc att aag cac ttc 931Gly Pro Glu Leu Ser Lys Asn Ser Val Ser Thr Thr Ile Lys His Phe
265 270 275cca ggt ggc ggc gtg cgc ctc gac ggc cac gat cct cac ttc cac tgg 979Pro Gly Gly Gly Val Arg Leu Asp Gly His Asp Pro His Phe His Trp
280 285 290ggt cag acc aat gag tac cca acc gaa gat gcg ctg ggc aag tac cat 1027Gly Gln Thr Asn Glu Tyr Pro Thr Glu Asp Ala Leu Gly Lys Tyr His
295 300 305ctg cct cct ttc cag gca gct atc gac gct ggc tgc gcc tcg atc atg 1075Leu Pro Pro Phe Gln Ala Ala Ile Asp Ala Gly Cys Ala Ser Ile Met310 315 320 325cct tac tac gca cgg cca atg aac aac tcc gcc aac cag ctc gat cag 1123Pro Tyr Tyr Ala Arg Pro Met Asn Asn Ser Ala Asn Gln Leu Asp Gln
330 335 340cag ctg tgg caa aac ccg acc acg cag ttc gaa gag gtt gcg ttt gcc 1171Gln Leu Trp Gln Asn Pro Thr Thr Gln Phe Glu Glu Val Ala Phe Ala
345 350 355tac aac cgc acc ttc att cag gat ttg ctt cgc gac gcc atg ggc cac 1219Tyr Asn Arg Thr Phe Ile Gln Asp Leu Leu Arg Asp Ala Met Gly His
360 365 370cgt ggg tac gtc aac tcc gac tcc ggc gtc atc gac gcc atg atg tgg 1267Arg Gly Tyr Val Asn Ser Asp Ser Gly Val Ile Asp Ala Met Met Trp
375 380 385ggc gtg gag gaa ctc agc gag cca gaa cgc ttc gcc gca gca gtg cgt 1315Gly Val Glu Glu Leu Ser Glu Pro Glu Arg Phe Ala Ala Ala Val Arg390 395 400 405gca ggc acc gac att ttc tcc gac atg gct aac cca cgt cga ctg ctc 1363Ala Gly Thr Asp Ile Phe Ser Asp Met Ala Asn Pro Arg Arg Leu Leu
410 415 420gaa gca gtt gct gag gga cac ctt gat gag tca gag ctg aat cag cca 1411Glu Ala Val Ala Glu Gly His Leu Asp Glu Ser Glu Leu Asn Gln Pro
425 430 435gtc cag cga ctc ctg gag gaa atc ttc cag ctt ggt ctg ttt gag aac 1459Val Gln Arg Leu Leu Glu Glu Ile Phe Gln Leu Gly Leu Phe Glu Asn
440 445 450cca tat gtc tct gaa gat gaa gca gaa aag atc att ggt gcg cca gag 1507Pro Tyr Val Ser Glu Asp Glu Ala Glu Lys Ile Ile Gly Ala Pro Glu
455 460 465gtt tct gca ttg ggc aac aaa gca cag ctt gat tcc gtc acc ttg ctg 1555Val Ser Ala Leu Gly Asn Lys Ala Gln Leu Asp Ser Val Thr Leu Leu470 475 480 485cgt aac aac ccc atc cgt gct gcc act gga tcc tgc agc aag cct gaa 1603Arg Asn Asn Pro Ile Arg Ala Ala Thr Gly Ser Cys Ser Lys Pro Glu
490 495 500gat cta ccc att ggt tac tgg ccg tac caa gat cga cga ggt tca act 1651Asp Leu Pro Ile Gly Tyr Trp Pro Tyr Gln Asp Arg Arg Gly Ser Thr
505 510 515aca gct gga agc agc cat tcg cgc aga act ccc agg ggt aac ctt ggt 1699Thr Ala Gly Ser Ser His Ser Arg Arg Thr Pro Arg Gly Asn Leu Gly
520 525 530gtc ttc cga gtc aga agc aga tct tgc aat cgt gtg ggc tcg ccc 1744Val Phe Arg Val Arg Ser Arg Ser Cys Asn Arg Val Gly Ser Pro
535 540 545tgaaattgca ctgtttgaag atg 1767<210>332<211>548<212>PRT<213>谷氨酸棒杆菌<400>332Met Ser Gln Glu Arg Pro Gln Ile Gly Ser Arg Leu Ser Arg Val Ile1 5 10 15Glu Gln Asp Gly Leu Gln Phe Arg Asp Leu Asp Gly Asp Gly Val Leu
20 25 30Ala Pro Tyr Glu Asp Trp Arg Leu Thr Pro Ala Glu Arg Ala Ala Asp
35 40 45Leu Val Lys Arg Met Asn Val Glu Glu Lys Ala Gly Leu Met Ile Ile
50 55 60Gly Ser His Tyr Pro Gly Tyr Ser Pro Leu Ala Pro Glu Ser Glu Gly65 70 75 80Lys Asp Ala Glu Lys Cys Glu Pro Leu Leu Asn Pro Val Asp Met Trp
85 90 95Arg Glu Asp Asn Pro Ile Thr Gly Val Pro Phe Thr Glu Pro Val Leu
100 105 110Ala Thr Ser Ser Thr Glu Asn Ala Ile Asn Leu Arg Asn Gln Arg Tyr
115 120 125Leu Ile Val Arg Asp Asn Leu Pro Ala Arg Gly Leu Ala Thr Trp Thr
130 135 140Asn Ala Val Gln Glu Val Ala Glu Arg Ser Arg Leu Gly Ile Pro Val145 150 155 160Ala Phe Ala Ser Asn Pro Arg Asn His Val Ala Leu Val Ala Gln Phe
165 170 175Gly Val Asn Glu Ser Ala Gly Val Phe Ser Glu Trp Pro Gly Glu Leu
180 185 190Gly Leu Ala Ala Leu Arg Asp Ala Glu Leu Met Glu Thr Phe Gly Thr
195 200 205Glu Ala Ala Lys Glu Trp Arg Ala Gly Gly Val His Lys Leu Tyr Gly
210 215 220Tyr Met Ala Asp Leu Ala Ser Glu Pro Arg Trp Ser Arg Phe Asn Gly225 230 235 240Thr Phe Gly Glu Asp Pro Glu Leu Ile Ser Asp Tyr Ile Ala Ala Val
245 250 255Val Arg Gly Leu Gln Gly Pro Glu Leu Ser Lys Asn Ser Val Ser Thr
260 265 270Thr Ile Lys His Phe Pro Gly Gly Gly Val Arg Leu Asp Gly His Asp
275 280 285Pro His Phe His Trp Gly Gln Thr Asn Glu Tyr Pro Thr Glu Asp Ala
290 295 300Leu Gly Lys Tyr His Leu Pro Pro Phe Gln Ala Ala Ile Asp Ala Gly305 310 315 320Cys Ala Ser Ile Met Pro Tyr Tyr Ala Arg Pro Met Asn Asn Ser Ala
325 330 335Asn Gln Leu Asp Gln Gln Leu Trp Gln Asn Pro Thr Thr Gln Phe Glu
340 345 350Glu Val Ala Phe Ala Tyr Asn Arg Thr Phe Ile Gln Asp Leu Leu Arg
355 360 365Asp Ala Met Gly His Arg Gly Tyr Val Asn Ser Asp Ser Gly Val Ile
370 375 380Asp Ala Met Met Trp Gly Val Glu Glu Leu Ser Glu Pro Glu Arg Phe385 390 395 400Ala Ala Ala Val Arg Ala Gly Thr Asp Ile Phe Ser Asp Met Ala Asn
405 410 415Pro Arg Arg Leu Leu Glu Ala Val Ala Glu Gly His Leu Asp Glu Ser
420 425 430Glu Leu Asn Gln Pro Val Gln Arg Leu Leu Glu Glu Ile Phe Gln Leu
435 440 445Gly Leu Phe Glu Asn Pro Tyr Val Ser Glu Asp Glu Ala Glu Lys Ile
450 455 460Ile Gly Ala Pro Glu Val Ser Ala Leu Gly Asn Lys Ala Gln Leu Asp465 470 475 480Ser Val Thr Leu Leu Arg Asn Asn Pro Ile Arg Ala Ala Thr Gly Ser
485 490 495Cys Ser Lys Pro Glu Asp Leu Pro Ile Gly Tyr Trp Pro Tyr Gln Asp
500 505 510Arg Arg Gly Ser Thr Thr Ala Gly Ser Ser His Ser Arg Arg Thr Pro
515 520 525Arg Gly Asn Leu Gly Val Phe Arg Val Arg Ser Arg Ser Cys Asn Arg
530 535 540Val Gly Ser Pro545<210>333<211>1607<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1584)<223>FRXA00799<400>333cta caa ttc cgc gat ctc gac ggc gac ggc gta ctt gca cct tat gaa 48Leu Gln Phe Arg Asp Leu Asp Gly Asp Gly Val Leu Ala Pro Tyr Glu1 5 10 15gat tgg cgt cta acc cca gca gag cgt gcc gct gac ctg gtg aaa cgt 96Asp Trp Arg Leu Thr Pro Ala Glu Arg Ala Ala Asp Leu Val Lys Arg
20 25 30atg aat gtg gaa gaa aaa gcg ggc ctg atg atc atc ggt tcg cac tac 144Met Asn Val Glu Glu Lys Ala Gly Leu Met Ile Ile Gly Ser His Tyr
35 40 45ccc gga tac tcg cct ttg gcg ccg gag agt gaa ggc aaa gac gcg gaa 192Pro Gly Tyr Ser Pro Leu Ala Pro Glu Ser Glu Gly Lys Asp Ala Glu
50 55 60aag tgc gag cct ttg ctg aac cct gtc gat atg tgg cgt gag gat aac 240Lys Cys Glu Pro Leu Leu Asn Pro Val Asp Met Trp Arg Glu Asp Asn65 70 75 80ccg atc acg ggt gtt cct ttc acc gag cct gtg ctg gca act tct tcc 288Pro Ile Thr Gly Val Pro Phe Thr Glu Pro Val Leu Ala Thr Ser Ser
85 90 95act gaa aat gcc att aac ctg cgc aat cag cgt tac tta att gtt cgt 336Thr Glu Asn Ala Ile Asn Leu Arg Asn Gln Arg Tyr Leu Ile Val Arg
100 105 110gac aac ctg cca gct cgt ggg ctt gct act tgg acc aat gct gtt cag 384Asp Asn Leu Pro Ala Arg Gly Leu Ala Thr Trp Thr Asn Ala Val Gln
115 120 125gaa gtc gcg gag cga tcc cgt ttg ggt att cct gtt gcg ttt gcg tcg 432Glu Val Ala Glu Arg Ser Arg Leu Gly Ile Pro Val Ala Phe Ala Ser
130 135 140aat cct cgt aac cac gtc gcg ctc gtt gcg cag ttc ggt gtg aac gag 480Asn Pro Arg Asn His Val Ala Leu Val Ala Gln Phe Gly Val Asn Glu145 150 155 160tcc gcg ggt gtg ttc tct gag tgg cct ggc gag ctg ggt ctt gct gcg 528Ser Ala Gly Val Phe Ser Glu Trp Pro Gly Glu Leu Gly Leu Ala Ala
165 170 175ctt cgc gat gct gaa ctg atg gag act ttc ggt acc gag gct gct aaa 576Leu Arg Asp Ala Glu Leu Met Glu Thr Phe Gly Thr Glu Ala Ala Lys
180 185 190gaa tgg cgt gcc ggt ggt gtg cac aag ctg tac ggt tac atg gct gac 624Glu Trp Arg Ala Gly Gly Val His Lys Leu Tyr Gly Tyr Met Ala Asp
195 200 205ctc gct tct gag cct cgt tgg tcc cgc ttc aac ggt act ttt ggt gag 672Leu Ala Ser Glu Pro Arg Trp Ser Arg Phe Asn Gly Thr Phe Gly Glu
210 215 220gat ccg gag ttg atc tct gat tac atc gct gct gtt gtg cgt ggt ttg 720Asp Pro Glu Leu Ile Ser Asp Tyr Ile Ala Ala Val Val Arg Gly Leu225 230 235 240cag ggc cct gag ctg tcc aag aat tcc gtg tcg acc acc att aag cac 768Gln Gly Pro Glu Leu Ser Lys Asn Ser Val Ser Thr Thr Ile Lys His
245 250 255ttc cca ggt ggc ggc gtg cgc ctc gac ggc cac gat cct cac ttc cac 816Phe Pro Gly Gly Gly Val Arg Leu Asp Gly His Asp Pro His Phe His
260 265 270tgg ggt cag acc aat gag tac cca acc gaa gat gcg ctg ggc aag tac 864Trp Gly Gln Thr Asn Glu Tyr Pro Thr Glu Asp Ala Leu Gly Lys Tyr
275 280 285cat ctg cct cct ttc cag gca gct atc gac gct ggc tgc gcc tcg atc 912His Leu Pro Pro Phe Gln Ala Ala Ile Asp Ala Gly Cys Ala Ser Ile
290 295 300atg cct tac tac gca cgg cca atg aac aac tcc gcc aac cag ctc gat 960Met Pro Tyr Tyr Ala Arg Pro Met Asn Asn Ser Ala Asn Gln Leu Asp305 310 315 320cag cag ctg tgg caa aac ccg acc acg cag ttc gaa gag gtt gcg ttt 1008Gln Gln Leu Trp Gln Asn Pro Thr Thr Gln Phe Glu Glu Val Ala Phe
325 330 335gcc tac aac cgc acc ttc att cag gat ttg ctt cgc gac gcc atg ggc 1056Ala Tyr Asn Arg Thr Phe Ile Gln Asp Leu Leu Arg Asp Ala Met Gly
340 345 350cac cgt ggg tac gtc aac tcc gac tcc ggc gtc atc gac gcc atg atg 1104His Arg Gly Tyr Val Asn Ser Asp Ser Gly Val Ile Asp Ala Met Met
355 360 365tgg ggc gtg gag gaa ctc agc gag cca gaa cgc ttc gcc gca gca gtg 1152Trp Gly Val Glu Glu Leu Ser Glu Pro Glu Arg Phe Ala Ala Ala Val
370 375 380cgt gca ggc acc gac att ttc tcc gac atg gct aac cca cgt cga ctg 1200Arg Ala Gly Thr Asp Ile Phe Ser Asp Met Ala Asn Pro Arg Arg Leu385 390 395 400ctc gaa gca gtt gct gag gga cac ctt gat gag tca gag ctg aat cag 1248Leu Glu Ala Val Ala Glu Gly His Leu Asp Glu Ser Glu Leu Asn Gln
405 410 415cca gtc cag cga ctc ctg gag gaa atc ttc cag ctt ggt ctg ttt gag 1296Pro Val Gln Arg Leu Leu Glu Glu Ile Phe Gln Leu Gly Leu Phe Glu
420 425 430aac cca tat gtc tct gaa gat gaa gca gaa aag atc att ggt gcg cca 1344Asn Pro Tyr Val Ser Glu Asp Glu Ala Glu Lys Ile Ile Gly Ala Pro
435 440 445gag gtt tct gca ttg ggc aac aaa gca cag ctt gat tcc gtc acc ttg 1392Glu Val Ser Ala Leu Gly Asn Lys Ala Gln Leu Asp Ser Val Thr Leu
450 455 460ctg cgt aac aac ccc atc cgt gct gcc act gga tcc tgc agc aag cct 1440Leu Arg Asn Asn Pro Ile Arg Ala Ala Thr Gly Ser Cys Ser Lys Pro465 470 475 480gaa gat cta ccc att ggt tac tgg ccg tac caa gat cga cga ggt tca 1488Glu Asp Leu Pro Ile Gly Tyr Trp Pro Tyr Gln Asp Arg Arg Gly Ser
485 490 495act aca gct gga agc agc cat tcg cgc aga act ccc agg ggt aac ctt 1536Thr Thr Ala Gly Ser Ser His Ser Arg Arg Thr Pro Arg Gly Asn Leu
500 505 510ggt gtc ttc cga gtc aga agc aga tct tgc aat cgt gtg ggc tcg ccc 1584Gly Val Phe Arg Val Arg Ser Arg Ser Cys Asn Arg Val Gly Ser Pro
515 520 525tgaaattgca ctgtttgaag atg 1607<210>334<211>528<212>PRT<213>谷氨酸棒杆菌<400>334Leu Gln Phe Arg Asp Leu Asp Gly Asp Gly Val Leu Ala Pro Tyr Glu1 5 10 15Asp Trp Arg Leu Thr Pro Ala Glu Arg Ala Ala Asp Leu Val Lys Arg
20 25 30Met Asn Val Glu Glu Lys Ala Gly Leu Met Ile Ile Gly Ser His Tyr
35 40 45Pro Gly Tyr Ser Pro Leu Ala Pro Glu Ser Glu Gly Lys Asp Ala Glu
50 55 60Lys Cys Glu Pro Leu Leu Asn Pro Val Asp Met Trp Arg Glu Asp Asn65 70 75 80Pro Ile Thr Gly Val Pro Phe Thr Glu Pro Val Leu Ala Thr Ser Ser
85 90 95Thr Glu Asn Ala Ile Asn Leu Arg Asn Gln Arg Tyr Leu Ile Val Arg
100 105 110Asp Asn Leu Pro Ala Arg Gly Leu Ala Thr Trp Thr Asn Ala Val Gln
115 120 125Glu Val Ala Glu Arg Ser Arg Leu Gly Ile Pro Val Ala Phe Ala Ser
130 135 140Asn Pro Arg Asn His Val Ala Leu Val Ala Gln Phe Gly Val Asn Glu145 150 155 160Ser Ala Gly Val Phe Ser Glu Trp Pro Gly Glu Leu Gly Leu Ala Ala
165 170 175Leu Arg Asp Ala Glu Leu Met Glu Thr Phe Gly Thr Glu Ala Ala Lys
180 185 190Glu Trp Arg Ala Gly Gly Val His Lys Leu Tyr Gly Tyr Met Ala Asp
195 200 205Leu Ala Ser Glu Pro Arg Trp Ser Arg Phe Asn Gly Thr Phe Gly Glu
210 215 220Asp Pro Glu Leu Ile Ser Asp Tyr Ile Ala Ala Val Val Arg Gly Leu225 230 235 240Gln Gly Pro Glu Leu Ser Lys Asn Ser Val Ser Thr Thr Ile Lys His
245 250 255Phe Pro Gly Gly Gly Val Arg Leu Asp Gly His Asp Pro His Phe His
260 265 270Trp Gly Gln Thr Asn Glu Tyr Pro Thr Glu Asp Ala Leu Gly Lys Tyr
275 280 285His Leu Pro Pro Phe Gln Ala Ala Ile Asp Ala Gly Cys Ala Ser Ile
290 295 300Met Pro Tyr Tyr Ala Arg Pro Met Asn Asn Ser Ala Asn Gln Leu Asp305 310 315 320Gln Gln Leu Trp Gln Asn Pro Thr Thr Gln Phe Glu Glu Val Ala Phe
325 330 335Ala Tyr Asn Arg Thr Phe Ile Gln Asp Leu Leu Arg Asp Ala Met Gly
340 345 350His Arg Gly Tyr Val Asn Ser Asp Ser Gly Val Ile Asp Ala Met Met
355 360 365Trp Gly Val Glu Glu Leu Ser Glu Pro Glu Arg Phe Ala Ala Ala Val
370 375 380Arg Ala Gly Thr Asp Ile Phe Ser Asp Met Ala Asn Pro Arg Arg Leu385 390 395 400Leu Glu Ala Val Ala Glu Gly His Leu Asp Glu Ser Glu Leu Asn Gln
405 410 415Pro Val Gln Arg Leu Leu Glu Glu Ile Phe Gln Leu Gly Leu Phe Glu
420 425 430Asn Pro Tyr Val Ser Glu Asp Glu Ala Glu Lys Ile Ile Gly Ala Pro
435 440 445Glu Val Ser Ala Leu Gly Asn Lys Ala Gln Leu Asp Ser Val Thr Leu
450 455 460Leu Arg Asn Asn Pro Ile Arg Ala Ala Thr Gly Ser Cys Ser Lys Pro465 470 475 480Glu Asp Leu Pro Ile Gly Tyr Trp Pro Tyr Gln Asp Arg Arg Gly Ser
485 490 495Thr Thr Ala Gly Ser Ser His Ser Arg Arg Thr Pro Arg Gly Asn Leu
500 505 510Gly Val Phe Arg Val Arg Ser Arg Ser Cys Asn Arg Val Gly Ser Pro
515 520 525<210>335<211>1632<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1609)<223>RXA00032<400>335cacttgctga agacgcccac atcgaagacc ttgcagatgt aaacgcaaac gcctaactgt 60ttttcgagct aaacccatcc ttgaaaggat cttttccacc atg aac acc cca ctc 115
Met Asn Thr Pro Leu
1 5cag ctc aac act gaa aac ctg cag gaa atc gct tcg act tcc gga gtg 163Gln Leu Asn Thr Glu Asn Leu Gln Glu Ile Ala Ser Thr Ser Gly Val
10 15 20cag atc cca gcg ttc aac cgc gct gac gtc gcc ccg ggc att gtc cac 211Gln Ile Pro Ala Phe Asn Arg Ala Asp Val Ala Pro Gly Ile Val His
25 30 35ttc ggt gtt ggc gga ttc cat cgc gct cac caa gcg atg tac ctc aat 259Phe Gly Val Gly Gly Phe His Arg Ala His Gln Ala Met Tyr Leu Asn
40 45 50gaa ttg atg aat gag ggc aag gcc ttg gat tgg ggc atc atc ggc atg 307Glu Leu Met Asn Glu Gly Lys Ala Leu Asp Trp Gly Ile Ile Gly Met
55 60 65ggt gtc atg cct tcc gat gtg cgc atg cgc gat gcc ctg gcc agc caa 355Gly Val Met Pro Ser Asp Val Arg Met Arg Asp Ala Leu Ala Ser Gln70 75 80 85gat cac ctt tat acc ctg acc act aaa gct cct gat gga act ctt gat 403Asp His Leu Tyr Thr Leu Thr Thr Lys Ala Pro Asp Gly Thr Leu Asp
90 95 100caa aaa atc atc gga tcc atc att gac tac gtg ttc gct ccc gag gac 451Gln Lys Ile Ile Gly Ser Ile Ile Asp Tyr Val Phe Ala Pro Glu Asp
105 110 115cca gca cgg gcc gtt gca acc ctc gcg cag gac tcc atc cgc att gtt 499Pro Ala Arg Ala Val Ala Thr Leu Ala Gln Asp Ser Ile Arg Ile Val
120 125 130tcc ctc acg gtg act gaa ggc gga tac aac atc gat ccg gcg aca gaa 547Ser Leu Thr Val Thr Glu Gly Gly Tyr Asn Ile Asp Pro Ala Thr Glu
135 140 145gat ttc gac cac acc aac cct cga atc gtt gct gac cgc gaa gcc ctg 595Asp Phe Asp His Thr Asn Pro Arg Ile Val Ala Asp Arg Glu Ala Leu150 155 160 165cag gcg ggc gat act tcc act ttg cag acc ttc ttt ggg ttg atc act 643Gln Ala Gly Asp Thr Ser Thr Leu Gln Thr Phe Phe Gly Leu Ile Thr
170 175 180gcc gca ttg att tcc cga aaa gaa tca gga tct acg cca ttt acc atc 691Ala Ala Leu Ile Ser Arg Lys Glu Ser Gly Ser Thr Pro Phe Thr Ile
185 190 195atg agc tgc gat aac atc caa ggc aac ggc gat ctg gct aag cgt ttc 739Met Ser Cys Asp Asn Ile Gln Gly Asn Gly Asp Leu Ala Lys Arg Phe
200 205 210ttc ctc gcc ttc gca cat tcc gtg tct tct gag ctc ggc gaa tgg gtg 787Phe Leu Ala Phe Ala His Ser Val Ser Ser Glu Leu Gly Glu Trp Val
215 220 225gaa aac aac gtg gcc ttc ccc aac tcc atg gtg gac cgc atc acc cct 835Glu Asn Asn Val Ala Phe Pro Asn Ser Met Val Asp Arg Ile Thr Pro230 235 240 245gaa acc acc gac ggc gac cgc gat gac atc aag gaa atc ggc tac atc 883Glu Thr Thr Asp Gly Asp Arg Asp Asp Ile Lys Glu Ile Gly Tyr Ile
250 255 260gat gcg tgg cca gtg gtt tct gaa gat ttc acc caa tgg gtc ctc gag 931Asp Ala Trp Pro Val Val Ser Glu Asp Phe Thr Gln Trp Val Leu Glu
265 270 275gat gcc ttc acc cag ggc cgc ccc gcg tac gag gag gtt ggc gtg cag 979Asp Ala Phe Thr Gln Gly Arg Pro Ala Tyr Glu Glu Val Gly Val Gln
280 285 290gtc gtc tcc gac gtg gag cct tat gaa tta atg aag ctg cgc ctg ctc 1027Val Val Ser Asp Val Glu Pro Tyr Glu Leu Met Lys Leu Arg Leu Leu
295 300 305aac gcc tcc cac cag gga ctt tgc tac ttc ggc cac ttg gct ggc cac 1075Asn Ala Ser His Gln Gly Leu Cys Tyr Phe Gly His Leu Ala Gly His310 315 320 325cac atg gtc cac gac gtc atg gcg gat acc cgc ttc cag gat ttc ctc 1123His Met Val His Asp Val Met Ala Asp Thr Arg Phe Gln Asp Phe Leu
330 335 340ctg gct tac atg gag cgc gaa gcc acc cct acc ctc aag gaa ctt cca 1171Leu Ala Tyr Met Glu Arg Glu Ala Thr Pro Thr Leu Lys Glu Leu Pro
345 350 355ggt gtc gat cta gat gct tat cga cgc caa ctc atc gcg cga ttc ggc 1219Gly Val Asp Leu Asp Ala Tyr Arg Arg Gln Leu Ile Ala Arg Phe Gly
360 365 370aac gcc gca gtc aaa gac acc gta ccg cgc ctg tgt gcg gaa tcc tcc 1267Asn Ala Ala Val Lys Asp Thr Val Pro Arg Leu Cys Ala Glu Ser Ser
375 380 385gac cgc att cca aag tgg ctg ttg cca gtc gta cgc gaa aac ctc gca 1315Asp Arg Ile Pro Lys Trp Leu Leu Pro Val Val Arg Glu Asn Leu Ala390 395 400 405gca ggc cgc gac gtc aca ctt tct gca gcc atc gtc gca tcc tgg gcg 1363Ala Gly Arg Asp Val Thr Leu Ser Ala Ala Ile Val Ala Ser Trp Ala
410 415 420cgc tac gca gaa ggc acc gac gag cag ggc aac cca ata aag att gtt 1411Arg Tyr Ala Glu Gly Thr Asp Glu Gln Gly Asn Pro Ile Lys Ile Val
425 430 435gac cgt ttg agt gag cgc gtc caa gaa aac gca tca gga aat cgc acc 1459Asp Arg Leu Ser Glu Arg Val Gln Glu Asn Ala Ser Gly Asn Arg Thr
440 445 450gat att ttg tca ttc atc cgc gac cgt gga atc ttc gga gac ttg gtc 1507Asp Ile Leu Ser Phe Ile Arg Asp Arg Gly Ile Phe Gly Asp Leu Val
455 460 465gat gct gaa cca ttc acc aag gca tac tcc gag aca ctg tcc tcc ctt 1555Asp Ala Glu Pro Phe Thr Lys Ala Tyr Ser Glu Thr Leu Ser Ser Leu470 475 480 485cat gac cgt ggc gcg gaa gca acc atc gat gca ctt ctt acg cag gta 1603His Asp Arg Gly Ala Glu Ala Thr Ile Asp Ala Leu Leu Thr Gln Val
490 495 500act gtc taaatccgtt gcgcgctagg gtt 1632Thr Val<210>336<211>503<212>PRT<213>谷氨酸棒杆菌<400>336Met Asn Thr Pro Leu Gln Leu Asn Thr Glu Asn Leu Gln Glu Ile Ala1 5 10 15Ser Thr Ser Gly Val Gln Ile Pro Ala Phe Asn Arg Ala Asp Val Ala
20 25 30Pro Gly Ile Val His Phe Gly Val Gly Gly Phe His Arg Ala His Gln
35 40 45Ala Met Tyr Leu Asn Glu Leu Met Asn Glu Gly Lys Ala Leu Asp Trp
50 55 60Gly Ile Ile Gly Met Gly Val Met Pro Ser Asp Val Arg Met Arg Asp65 70 75 80Ala Leu Ala Ser Gln Asp His Leu Tyr Thr Leu Thr Thr Lys Ala Pro
85 90 95Asp Gly Thr Leu Asp Gln Lys Ile Ile Gly Ser Ile Ile Asp Tyr Val
100 105 110Phe Ala Pro Glu Asp Pro Ala Arg Ala Val Ala Thr Leu Ala Gln Asp
115 120 125Ser Ile Arg Ile Val Ser Leu Thr Val Thr Glu Gly Gly Tyr Asn Ile
130 135 140Asp Pro Ala Thr Glu Asp Phe Asp His Thr Asn Pro Arg Ile Val Ala145 150 155 160Asp Arg Glu Ala Leu Gln Ala Gly Asp Thr Ser Thr Leu Gln Thr Phe
165 170 175Phe Gly Leu Ile Thr Ala Ala Leu Ile Ser Arg Lys Glu Ser Gly Ser
180 185 190Thr Pro Phe Thr Ile Met Ser Cys Asp Asn Ile Gln Gly Asn Gly Asp
195 200 205Leu Ala Lys Arg Phe Phe Leu Ala Phe Ala His Ser Val Ser Ser Glu
210 215 220Leu Gly Glu Trp Val Glu Asn Asn Val Ala Phe Pro Asn Ser Met Val225 230 235 240Asp Arg Ile Thr Pro Glu Thr Thr Asp Gly Asp Arg Asp Asp Ile Lys
245 250 255Glu Ile Gly Tyr Ile Asp Ala Trp Pro Val Val Ser Glu Asp Phe Thr
260 265 270Gln Trp Val Leu Glu Asp Ala Phe Thr Gln Gly Arg Pro Ala Tyr Glu
275 280 285Glu Val Gly Val Gln Val Val Ser Asp Val Glu Pro Tyr Glu Leu Met
290 295 300Lys Leu Arg Leu Leu Asn Ala Ser His Gln Gly Leu Cys Tyr Phe Gly305 310 315 320His Leu Ala Gly His His Met Val His Asp Val Met Ala Asp Thr Arg
325 330 335Phe Gln Asp Phe Leu Leu Ala Tyr Met Glu Arg Glu Ala Thr Pro Thr
340 345 350Leu Lys Glu Leu Pro Gly Val Asp Leu Asp Ala Tyr Arg Arg Gln Leu
355 360 365Ile Ala Arg Phe Gly Asn Ala Ala Val Lys Asp Thr Val Pro Arg Leu
370 375 380Cys Ala Glu Ser Ser Asp Arg Ile Pro Lys Trp Leu Leu Pro Val Val385 390 395 400Arg Glu Asn Leu Ala Ala Gly Arg Asp Val Thr Leu Ser Ala Ala Ile
405 410 415Val Ala Ser Trp Ala Arg Tyr Ala Glu Gly Thr Asp Glu Gln Gly Asn
420 425 430Pro Ile Lys Ile Val Asp Arg Leu Ser Glu Arg Val Gln Glu Asn Ala
435 440 445Ser Gly Asn Arg Thr Asp Ile Leu Ser Phe Ile Arg Asp Arg Gly Ile
450 455 460Phe Gly Asp Leu Val Asp Ala Glu Pro Phe Thr Lys Ala Tyr Ser Glu465 470 475 480Thr Leu Ser Ser Leu His Asp Arg Gly Ala Glu Ala Thr Ile Asp Ala
485 490 495Leu Leu Thr Gln Val Thr Val
500<210>337<211>1098<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1075)<223>RXA02528<400>337ctggcgcggt atcgattaca atctggttgg ttatcggtgg tttgtggatg aatgtcatcg 60gactctggta atcgaaaatt aaaggtaagg gggtgtggag atg tca gca aaa tcg 115
Met Ser Ala Lys Ser
1 5agc ctc aag gaa gtt gct gag tta gct gga gtc ggt tat gcc aca gcc 163Ser Leu Lys Glu Val Ala Glu Leu Ala Gly Val Gly Tyr Ala Thr Ala
10 15 20tcg agg gca cta tct ggc aag ggg tat gtg tcc ccg cag acg cgg gag 211Ser Arg Ala Leu Ser Gly Lys Gly Tyr Val Ser Pro Gln Thr Arg Glu
25 30 35aaa gtt cag gcg gcg gct aaa gag ctg aac tat gta cca aat cag ctg 259Lys Val Gln Ala Ala Ala Lys Glu Leu Asn Tyr Val Pro Asn Gln Leu
40 45 50gcc aag gcg ttg cgg gaa cat cgc agt gcc ttg gtg ggg gtc att gtt 307Ala Lys Ala Leu Arg Glu His Arg Ser Ala Leu Val Gly Val Ile Val
55 60 65ccg gat ttg tcc aat gag tat tat tcg gaa tcg ctg cag act att cag 355Pro Asp Leu Ser Asn Glu Tyr Tyr Ser Glu Ser Leu Gln Thr Ile Gln70 75 80 85cag gat ctg aaa gct gct ggc tat caa atg ctg gtt gcg gag gcc aac 403Gln Asp Leu Lys Ala Ala Gly Tyr Gln Met Leu Val Ala Glu Ala Asn
90 95 100agt gtg cag gcg cag gac gtg gtg atg gaa tcg ttg atc tcg att caa 451Ser Val Gln Ala Gln Asp Val Val Met Glu Ser Leu Ile Ser Ile Gln
105 110 115gct gca gga att arc cac gtt cca gtg gtc ggc rca att gct cct gaa 499Ala Ala Gly Ile Ile His Val Pro Val Val Gly Ser Ile Ala Pro Glu
120 125 130gga atc ccc atg gtg cag ttg act cgt ggt gaa ttg ggt cct ggt ttc 547Gly Ile Pro Met Val Gln Leu Thr Arg Gly Glu Leu Gly Pro Gly Phe
135 140 145cct cgg gtg ttg tgt gat gat gag gct ggg ttt ttt cag ctg acc gag 595Pro Arg Val Leu Cys Asp Asp Glu Ala Gly Phe Phe Gln Leu Thr Glu150 155 160 165tcg gtg ctg ggc ggc agc gga atg aac att gct gct ttg gtt ggt gaa 643Ser Val Leu Gly Gly Ser Gly Met Asn Ile Ala Ala Leu Val Gly Glu
170 175 180gaa tca ctt tcc acc acg cag gaa cga atg cgc ggt att agt cat gcg 691Glu Ser Leu Ser Thr Thr Gln Glu Arg Met Arg Gly Ile Ser His Ala
185 190 195gcg tcg ata tat ggg gct gag gtg acg ttc cat ttt ggc cac tat tct 739Ala Ser Ile Tyr Gly Ala Glu Val Thr Phe His Phe Gly His Tyr Ser
200 205 210gtc gaa tct ggc gaa gag atg gct cag gtg gtg ttt aac aac ggc ctt 787Val Glu Ser Gly Glu Glu Met Ala Gln Val Val Phe Asn Asn Gly Leu
215 220 225ccc gat gca ttg att gtg gcg tct cct cgg ctg atg gct ggg gtg atg 835Pro Asp Ala Leu Ile Val Ala Ser Pro Arg Leu Met Ala Gly Val Met230 235 240 245cgt gct ttt act cgc ctg aat gtc cgc gtt ccc cac gat gtg gtg att 883Arg Ala Phe Thr Arg Leu Asn Val Arg Val Pro His Asp Val Val Ile
250 255 260ggt ggt tat gac gat cct gag tgg tac agc ttt gtc ggc gcg ggg att 931Gly Gly Tyr Asp Asp Pro Glu Trp Tyr Ser Phe Val Gly Ala Gly Ile
265 270 275acc acg ttt gtt cca ccg cat gag gag atg ggg aaa gag gcc gtg cgc 979Thr Thr Phe Val Pro Pro His Glu Glu Met Gly Lys Glu Ala Val Arg
280 285 290ttg ttg gta gat ctg att gaa aat ccc gaa ctt ccc acc ggc gat gtg 1027Leu Leu Val Asp Leu Ile Glu Asn Pro Glu Leu Pro Thr Gly Asp Val
295 300 305gtt ttg cag ggg cag gtg atc ctt cgg ggg tcg agc aca cat tcc ggg 1075Val Leu Gln Gly Gln Val Ile Leu Arg Gly Ser Ser Thr His Ser Gly310 315 320 325tagaattgcc caaatgtcat caa 1098<210>338<211>325<212>PRT<213>谷氨酸棒杆菌<400>338Met Ser Ala Lys Ser Ser Leu Lys Glu Val Ala Glu Leu Ala Gly Val1 5 10 15Gly Tyr Ala Thr Ala Ser Arg Ala Leu Ser Gly Lys Gly Tyr Val Ser
20 25 30Pro Gln Thr Arg Glu Lys Val Gln Ala Ala Ala Lys Glu Leu Asn Tyr
35 40 45Val Pro Asn Gln Leu Ala Lys Ala Leu Arg Glu His Arg Ser Ala Leu
50 55 60Val Gly Val Ile Val Pro Asp Leu Ser Asn Glu Tyr Tyr Ser Glu Ser65 70 75 80Leu Gln Thr Ile Gln Gln Asp Leu Lys Ala Ala Gly Tyr Gln Met Leu
85 90 95Val Ala Glu Ala Asn Ser Val Gln Ala Gln Asp Val Val Met Glu Ser
100 105 110Leu Ile Ser Ile Gln Ala Ala Gly Ile Ile His Val Pro Val Val Gly
115 120 125Ser Ile Ala Pro Glu Gly Ile Pro Met Val Gln Leu Thr Arg Gly Glu
130 135 140Leu Gly Pro Gly Phe Pro Arg Val Leu Cys Asp Asp Glu Ala Gly Phe145 150 155 160Phe Gln Leu Thr Glu Ser Val Leu Gly Gly Ser Gly Met Asn Ile Ala
165 170 175Ala Leu Val Gly Glu Glu Ser Leu Ser Thr Thr Gln Glu Arg Met Arg
180 185 190Gly Ile Ser His Ala Ala Ser Ile Tyr Gly Ala Glu Val Thr Phe His
195 200 205Phe Gly His Tyr Ser Val Glu Ser Gly Glu Glu Met Ala Gln Val Val
210 215 220Phe Asn Asn Gly Leu Pro Asp Ala Leu Ile Val Ala Ser Pro Arg Leu225 230 235 240Met Ala Gly Val Met Arg Ala Phe Thr Arg Leu Asn Val Arg Val Pro
245 250 255His Asp Val Val Ile Gly Gly Tyr Asp Asp Pro Glu Trp Tyr Ser Phe
260 265 270Val Gly Ala Gly Ile Thr Thr Phe Val Pro Pro His Glu Glu Met Gly
275 280 285Lys Glu Ala Val Arg Leu Leu Val Asp Leu Ile Glu Asn Pro Glu Leu
290 295 300Pro Thr Gly Asp Val Val Leu Gln Gly Gln Val Ile Leu Arg Gly Ser305 310 315 320Ser Thr His Ser Gly
325<210>339<211>1246<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1246)<223>RXN00316<400>339agcgcatgat cgatgcctgc gagtccgcag gggtccaact tggcgtgctc ttccagcgcc 60gcttctggcc cgcggctcaa aaaatgaaaa aggagccgtc atg ggc caa tgc acg 115
Met Gly Gln Cys Thr
1 5gta gcg ctt tac cga gag cat tcc tat tac aca gca acc cca tgg cga 163Val Ala Leu Tyr Arg Glu His Ser Tyr Tyr Thr Ala Thr Pro Trp Arg
10 15 20gga acc tgg gca gcc gat ggc ggt gga gtg ctc atg act caa gcc atc 211Gly Thr Trp Ala Ala Asp Gly Gly Gly Val Leu Met Thr Gln Ala Ile
25 30 35cac tac atc gat ctt ttg tac tgg ctg ttg ggc gaa ccc gtg gaa gtt 259His Tyr Ile Asp Leu Leu Tyr Trp Leu Leu Gly Glu Pro Val Glu Val
40 45 50ttc ggc tac acc aac tcc ttc aaa cac ggc gac aac atc gaa gtc gaa 307Phe Gly Tyr Thr Asn Ser Phe Lys His Gly Asp Asn Ile Glu Val Glu
55 60 65gac agc gcc gtt gcc act gtg cgt ttt gaa tcg ggc gcg ttg gcc aca 355Asp Ser Ala Val Ala Thr Val Arg Phe Glu Ser Gly Ala Leu Ala Thr70 75 80 85att tca gcc acc acc gcc gca gag cca gca ctc ggc gca caa gtg cag 403Ile Ser Ala Thr Thr Ala Ala Glu Pro Ala Leu Gly Ala Gln Val Gln
90 95 100gtg atg gga aca aag ggt gcc acc atg acg atc ctg gaa ttc cct gaa 451Val Met Gly Thr Lys Gly Ala Thr Met Thr Ile Leu Glu Phe Pro Glu
105 110 115ggt acc gac ggc agg ctc att gtt cgc agt gaa aac gac acc cgt cga 499Gly Thr Asp Gly Arg Leu Ile Val Arg Ser Glu Asn Asp Thr Arg Arg
120 125 130aac cac ccc att cca ccc cgc gga tct tta tcc caa tgc cga tct ttc 547Asn His Pro Ile Pro Pro Arg Gly Ser Leu Ser Gln Cys Arg Ser Phe
135 140 145cat cat caa cgg tgc ttt gat ccc gta tca cac cgc cca gat cgc aga 595His His Gln Arg Cys Phe Asp Pro Val Ser His Arg Pro Asp Arg Arg150 155 160 165ctt tat cga tgc gct caa cga agg ccg ccc acc act gat cac cgg ccg 643Leu Tyr Arg Cys Ala Gln Arg Arg Pro Pro Thr Thr Asp His Arg Pro
170 175 180cga tgc cac cag agc tct gaa agt tct cct tgg tgt cta cga atc agc 691Arg Cys His Gln Ser Ser Glu Ser Ser Pro Trp Cys Leu Arg Ile Ser
185 190 195agc cac cca cca gcc ggt ctc ttt gat cta acg gaa gct ttt aaa acg 739Ser His Pro Pro Ala Gly Leu Phe Asp Leu Thr Glu Ala Phe Lys Thr
200 205 210tca cgc caa atc ggt ctt gca cct tta tcc tcc ctg tcc aca cca cct 787Ser Arg Gln Ile Gly Leu Ala Pro Leu Ser Ser Leu Ser Thr Pro Pro
215 220 225gat caa cta gtg cgc ctc gca gct gcc act ggt ttc tcc ttt gtc ggt 835Asp Gln Leu Val Arg Leu Ala Ala Ala Thr Gly Phe Ser Phe Val Gly230 235 240 245ctg cgc gtc atc gca gta acc ccc aac gaa cgt gta tat gac ctt tcc 883Leu Arg Val Ile Ala Val Thr Pro Asn Glu Arg Val Tyr Asp Leu Ser
250 255 260cca gga tcc cca ctg ctg gct gca acc caa caa gcg ttg aaa gaa acc 931Pro Gly Ser Pro Leu Leu Ala Ala Thr Gln Gln Ala Leu Lys Glu Thr
265 270 275gcc ctg tat gtg ctc gac act gaa ttc cta cag gta aac gca gac acc 979Ala Leu Tyr Val Leu Asp Thr Glu Phe Leu Gln Val Asn Ala Asp Thr
280 285 290acc cgc gag gcc tgg ctt ccc gca cta gaa gcc gcc gga gca ctg gga 1027Thr Arg Glu Ala Trp Leu Pro Ala Leu Glu Ala Ala Gly Ala Leu Gly
295 300 305gct aaa acc ttc acc atc gcc gcc ggt gat gac aac att gcg ccc ctg 1075Ala Lys Thr Phe Thr Ile Ala Ala Gly Asp Asp Asn Ile Ala Pro Leu310 315 320 325acc gac acg atc ggt gcc atg gtt gac gat gcc cgt gat ttc gga gtc 1123Thr Asp Thr Ile Gly Ala Met Val Asp Asp Ala Arg Asp Phe Gly Val
330 335 340acc cca gcc cta gag cca atc tct tac cgc agc gtg cat tcc att ccg 1171Thr Pro Ala Leu Glu Pro Ile Ser Tyr Arg Ser Val His Ser Ile Pro
345 350 355cag gca gca gca atc gcc aga gac tcc ggc gga aaa gtc gtg gcg gac 1219Gln Ala Ala Ala Ile Ala Arg Asp Ser Gly Gly Lys Val Val Ala Asp
360 365 370acc ttg cac atg gcc agg ttc gga gcc 1246Thr Leu His Met Ala Arg Phe Gly Ala
375 380<210>340<211>382<212>PRT<213>谷氨酸棒杆菌<400>340Met Gly Gln Cys Thr Val Ala Leu Tyr Arg Glu His Ser Tyr Tyr Thr1 5 10 15Ala Thr Pro Trp Arg Gly Thr Trp Ala Ala Asp Gly Gly Gly Val Leu
20 25 30Met Thr Gln Ala Ile His Tyr Ile Asp Leu Leu Tyr Trp Leu Leu Gly
35 40 45Glu Pro Val Glu Val Phe Gly Tyr Thr Asn Ser Phe Lys His Gly Asp
50 55 60Asn Ile Glu Val Glu Asp Ser Ala Val Ala Thr Val Arg Phe Glu Ser65 70 75 80Gly Ala Leu Ala Thr Ile Ser Ala Thr Thr Ala Ala Glu Pro Ala Leu
85 90 95Gly Ala Gln Val Gln Val Met Gly Thr Lys Gly Ala Thr Met Thr Ile
100 105 110Leu Glu Phe Pro Glu Gly Thr Asp Gly Arg Leu Ile Val Arg Ser Glu
115 120 125Asn Asp Thr Arg Arg Asn His Pro Ile Pro Pro Arg Gly Ser Leu Ser
130 135 140Gln Cys Arg Ser Phe His His Gln Arg Cys Phe Asp Pro Val Ser His145 150 155 160Arg Pro Asp Arg Arg Leu Tyr Arg Cys Ala Gln Arg Arg Pro Pro Thr
165 170 175Thr Asp His Arg Pro Arg Cys His Gln Ser Ser Glu Ser Ser Pro Trp
180 185 190Cys Leu Arg Ile Ser Ser His Pro Pro Ala Gly Leu Phe Asp Leu Thr
195 200 205Glu Ala Phe Lys Thr Ser Arg Gln Ile Gly Leu Ala Pro Leu Ser Ser
210 215 220Leu Ser Thr Pro Pro Asp Gln Leu Val Arg Leu Ala Ala Ala Thr Gly225 230 235 240Phe Ser Phe Val Gly Leu Arg Val Ile Ala Val Thr Pro Asn Glu Arg
245 250 255Val Tyr Asp Leu Ser Pro Gly Ser Pro Leu Leu Ala Ala Thr Gln Gln
260 265 270Ala Leu Lys Glu Thr Ala Leu Tyr Val Leu Asp Thr Glu Phe Leu Gln
275 280 285Val Asn Ala Asp Thr Thr Arg Glu Ala Trp Leu Pro Ala Leu Glu Ala
290 295 300Ala Gly Ala Leu Gly Ala Lys Thr Phe Thr Ile Ala Ala Gly Asp Asp305 310 315 320Asn Ile Ala Pro Leu Thr Asp Thr Ile Gly Ala Met Val Asp Asp Ala
325 330 335Arg Asp Phe Gly Val Thr Pro Ala Leu Glu Pro Ile Ser Tyr Arg Ser
340 345 350Val His Ser Ile Pro Gln Ala Ala Ala Ile Ala Arg Asp Ser Gly Gly
355 360 365Lys Val Val Ala Asp Thr Leu His Met Ala Arg Phe Gly Ala
370 375 380<210>341<211>412<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(412)<223>FRXA00309<400>341agcgcatgat cgatgcctgc gagtccgcag gggtccaact tggcgtgctc ttccagcgcc 60gcttctggcc cgcggctcaa aaaatgaaaa aggagccgtc atg ggc caa tgc acg 115
Met Gly Gln Cys Thr
1 5gta gcg ctt tac cga gag cat tcc tat tac aca gca acc cca tgg cga 163Val Ala Leu Tyr Arg Glu His Ser Tyr Tyr Thr Ala Thr Pro Trp Arg
10 15 20gga acc tgg gca gcc gat ggc ggt gga gtg ctc atg act caa gcc atc 211Gly Thr Trp Ala Ala Asp Gly Gly Gly Val Leu Met Thr Gln Ala Ile
25 30 35cac tac atc gat ctt ttg tac tgg ctg ttg ggc gaa ccc gtg gaa gtt 259His Tyr Ile Asp Leu Leu Tyr Trp Leu Leu Gly Glu Pro Val Glu Val
40 45 50ttc ggc tac acc aac tcc ttc aaa cac ggc gac aac atc gaa gtc gaa 307Phe Gly Tyr Thr Asn Ser Phe Lys His Gly Asp Asn Ile Glu Val Glu
55 60 65gac agc gcc gtt gcc act gtg cgt ttt gaa tcg ggc gcg ttg gcc aca 355Asp Ser Ala Val Ala Thr Val Arg Phe Glu Ser Gly Ala Leu Ala Thr70 75 80 85att tca gcc acc acc gcc gca gag cca gca ctc ggc gca caa gtg cag 403Ile Ser Ala Thr Thr Ala Ala Glu Pro Ala Leu Gly Ala Gln Val Gln
90 95 100gtg atg gga 412Val Met Gly<210>342<211>104<212>PRT<213>谷氨酸棒杆菌<400>342Met Gly Gln Cys Thr Val Ala Leu Tyr Arg Glu His Ser Tyr Tyr Thr1 5 10 15Ala Thr Pro Trp Arg Gly Thr Trp Ala Ala Asp Gly Gly Gly Val Leu
20 25 30Met Thr Gln Ala Ile His Tyr Ile Asp Leu Leu Tyr Trp Leu Leu Gly
35 40 45Glu Pro Val Glu Val Phe Gly Tyr Thr Asn Ser Phe Lys His Gly Asp
50 55 60Asn Ile Glu Val Glu Asp Ser Ala Val Ala Thr Val Arg Phe Glu Ser65 70 75 80Gly Ala Leu Ala Thr Ile Ser Ala Thr Thr Ala Ala Glu Pro Ala Leu
85 90 95Gly Ala Gln Val Gln Val Met Gly
100<210>343<211>558<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(535)<223>RXN00310<400>343ttgcgggatt catcatcggc gcaatcgcac tgtctgccgc agttattttg accaccaagg 60aaaccgcctt caccaagctt gaagatctag ggaagaaata atg tct gac aag atc 115
Met Ser Asp Lys Ile
1 5tgg aaa gtc ggc atc atc ggt tgc ggt gca atc agc cga aac cat atc 163Trp Lys Val Gly Ile Ile Gly Cys Gly Ala Ile Ser Arg Asn His Ile
10 15 20gaa gca gtt cag gca atc ccc ggc gca gaa gtc agc gca gtc tgt gat 211Glu Ala Val Gln Ala Ile Pro Gly Ala Glu Val Ser Ala Val Cys Asp
25 30 35gtg gat ggt gcg aaa gca tcg gaa acc gca gcg aaa tat gga att tct 259Val Asp Gly Ala Lys Ala Ser Glu Thr Ala Ala Lys Tyr Gly Ile Ser
40 45 50ccc agt ttc acg tct gtc gat gag atc ctc gcc tcc ggg gtg gac att 307Pro Ser Phe Thr Ser Val Asp Glu Ile Leu Ala Ser Gly Val Asp Ile
55 60 65gtc gca gtc tgc acc cca cat cca acc cac gaa aca gtg gtc ctc gcc 355Val Ala Val Cys Thr Pro His Pro Thr His Glu Thr Val Val Leu Ala70 75 80 85gct gct gcc gcc gga gtg cac gtg ctt tgt gag aag cca atc gcc atc 403Ala Ala Ala Ala Gly Val His Val Leu Cys Glu Lys Pro Ile Ala Ile
90 95 100gaa ctc gat tcc gca cag cgc atg atc gat gcc tgc gag tcc gca ggg 451Glu Leu Asp Ser Ala Gln Arg Met Ile Asp Ala Cys Glu Ser Ala Gly
105 110 115gtc caa ctt ggc gtg ctc ttc cag cgc cgc ttc tgg ccc gcg gct caa 499Val Gln Leu Gly Val Leu Phe Gln Arg Arg Phe Trp Pro Ala Ala Gln
120 125 130aaa atg aaa aag gag ccg tca tgg gcc aat gca cgg tagcgcttta 545Lys Met Lys Lys Glu Pro Ser Trp Ala Asn Ala Arg
135 140 145ccgagagcat tcc 558<210>344<211>145<212>PRT<213>谷氨酸棒杆菌<400>344Met Ser Asp Lys Ile Trp Lys Val Gly Ile Ile Gly Cys Gly Ala Ile1 5 10 15Ser Arg Asn His Ile Glu Ala Val Gln Ala Ile Pro Gly Ala Glu Val
20 25 30Ser Ala Val Cys Asp Val Asp Gly Ala Lys Ala Ser Glu Thr Ala Ala
35 40 45Lys Tyr Gly Ile Ser Pro Ser Phe Thr Ser Val Asp Glu Ile Leu Ala
50 55 60Ser Gly Val Asp Ile Val Ala Val Cys Thr Pro His Pro Thr His Glu65 70 75 80Thr Val Val Leu Ala Ala Ala Ala Ala Gly Val His Val Leu Cys Glu
85 90 95Lys Pro Ile Ala Ile Glu Leu Asp Ser Ala Gln Arg Met Ile Asp Ala
100 105 110Cys Glu Ser Ala Gly Val Gln Leu Gly Val Leu Phe Gln Arg Arg Phe
115 120 125Trp Pro Ala Ala Gln Lys Met Lys Lys Glu Pro Ser Trp Ala Asn Ala
130 135 140Arg145<210>345<211>558<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(535)<223>FRXA00310<400>345ttgcgggatt catcatcggc gcaatcgcac tgtctgccgc agttattttg accaccaagg 60aaaccgcctt caccaagctt gaagatctag ggaagaaata atg tct gac aag atc 115
Met Ser Asp Lys Ile
1 5tgg aaa gtc ggc atc atc ggt tgc ggt gca atc agc cga aac cat atc 163Trp Lys Val Gly Ile Ile Gly Cys Gly Ala Ile Ser Arg Asn His Ile
10 15 20gaa gca gtt cag gca atc ccc ggc gca gaa gtc agc gca gtc tgt gat 211Glu Ala Val Gln Ala Ile Pro Gly Ala Glu Val Ser Ala Val Cys Asp
25 30 35gtg gat ggt gcg aaa gca tcg gaa acc gca gcg aaa tat gga att tct 259Val Asp Gly Ala Lys Ala Ser Glu Thr Ala Ala Lys Tyr Gly Ile Ser
40 45 50ccc agt ttc acg tct gtc gat gag atc ctc gcc tcc ggg gtg gac att 307Pro Ser Phe Thr Ser Val Asp Glu Ile Leu Ala Ser Gly Val Asp Ile
55 60 65gtc gca gtc tgc acc cca cat cca acc cac gaa aca gtg gtc ctc gcc 355Val Ala Val Cys Thr Pro His Pro Thr His Glu Thr Val Val Leu Ala70 75 80 85gct gct gcc gcc gga gtg cac gtg ctt tgt gag aag cca atc gcc atc 403Ala Ala Ala Ala Gly Val His Val Leu Cys Glu Lys Pro Ile Ala Ile
90 95 100gaa ctc gat tcc gca cag cgc atg atc gat gcc tgc gag tcc gca ggg 451Glu Leu Asp Ser Ala Gln Arg Met Ile Asp Ala Cys Glu Ser Ala Gly
105 110 115gtc caa ctt ggc gtg ctc ttc cag cgc cgc ttc tgg ccc gcg gct caa 499Val Gln Leu Gly Val Leu Phe Gln Arg Arg Phe Trp Pro Ala Ala Gln
120 125 130aaa atg aaa aag gag ccg tca tgg gcc aat gca cgg tagcgcttta 545Lys Met Lys Lys Glu Pro Ser Trp Ala Asn Ala Arg
135 140 145ccgagagcat tcc 558<210>346<211>145<212>PRT<213>谷氨酸棒杆菌<400>346Met Ser Asp Lys Ile Trp Lys Val Gly Ile Ile Gly Cys Gly Ala Ile1 5 10 15Ser Arg Asn His Ile Glu Ala Val Gln Ala Ile Pro Gly Ala Glu Val
20 25 30Ser Ala Val Cys Asp Val Asp Gly Ala Lys Ala Ser Glu Thr Ala Ala
35 40 45Lys Tyr Gly Ile Ser Pro Ser Phe Thr Ser Val Asp Glu Ile Leu Ala
50 55 60Ser Gly Val Asp Ile Val Ala Val Cys Thr Pro His Pro Thr His Glu65 70 75 80Thr Val Val Leu Ala Ala Ala Ala Ala Gly Val His Val Leu Cys Glu
85 90 95Lys Pro Ile Ala Ile Glu Leu Asp Ser Ala Gln Arg Met Ile Asp Ala
100 105 110Cys Glu Ser Ala Gly Val Gln Leu Gly Val Leu Phe Gln Arg Arg Phe
115 120 125Trp Pro Ala Ala Gln Lys Met Lys Lys Glu Pro Ser Trp Ala Asn Ala
130 135 140Arg145<210>347<211>1342<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1342)<223>RXA00041<400>347atgaagcagc agcatccaag ctggaaaacg ctgatcacta ccgtctcatg gagcaattaa 60agctgcgcta gaaacaaaaa ggaaagtagt gtgtggggct atg cac aca gaa ctt 115
Met His Thr Glu Leu
1 5tcc agt ttg cgc cct gcg tac cat gtg act cct ccg cag ggc agg ctc 163Ser Ser Leu Arg Pro Ala Tyr His Val Thr Pro Pro Gln Gly Arg Leu
10 15 20aat gat ccc aac gga atg tac gtc gat ggc gat acc ctc cac gtc tac 211Asn Asp Pro Asn Gly Met Tyr Val Asp Gly Asp Thr Leu His Val Tyr
25 30 35tac cag cac gat cca ggt ttc ccc ttc gca cca aag cgc acc ggc tgg 259Tyr Gln His Asp Pro Gly Phe Pro Phe Ala Pro Lys Arg Thr Gly Trp
40 45 50gct cac acc acc acg ccg ttg acc gga ccg cag cga ttg cag tgg acg 307Ala His Thr Thr Thr Pro Leu Thr Gly Pro Gln Arg Leu Gln Trp Thr
55 60 65cac ctg ccc gac gct ctt tac ccg gat gca tcc tat gac ctg gat gga 355His Leu Pro Asp Ala Leu Tyr Pro Asp Ala Ser Tyr Asp Leu Asp Gly70 75 80 85tgc tat tcc ggt gga gcc gta ttt act gac ggc aca ctt aaa ctt ttc 403Cys Tyr Ser Gly Gly Ala Val Phe Thr Asp Gly Thr Leu Lys Leu Phe
90 95 100tac acc ggc aac cta aaa att gac ggc aag cgc cgc gcc acc caa aac 451Tyr Thr Gly Asn Leu Lys Ile Asp Gly Lys Arg Arg Ala Thr Gln Asn
105 110 115ctc gtc gaa gtc gag gac cca act ggg ctg atg ggc ggc att cat cgc 499Leu Val Glu Val Glu Asp Pro Thr Gly Leu Met Gly Gly Ile His Arg
120 125 130cgt tcg cct aaa aat ccg ctt atc gac gga ccc gcc agc ggt ttc aca 547Arg Ser Pro Lys Asn Pro Leu Ile Asp Gly Pro Ala Ser Gly Phe Thr
135 140 145ccc cat tac cgc gat ccc atg atc agc cct gat ggt gat ggt tgg aaa 595Pro His Tyr Arg Asp Pro Met Ile Ser Pro Asp Gly Asp Gly Trp Lys150 155 160 165atg gtt ctt ggg gcc caa cgc gaa aac ctc acc ggt gca gcg gtt cta 643Met Val Leu Gly Ala Gln Arg Glu Asn Leu Thr Gly Ala Ala Val Leu
170 175 180tac cgc tcg aca gat ctt gaa aac tgg gaa ttc tcc ggt gaa atc acc 691Tyr Arg Ser Thr Asp Leu Glu Asn Trp Glu Phe Ser Gly Glu Ile Thr
185 190 195ttt gac ctc agt gat gca caa cct ggt tct gct cct gat ctc gtt ccc 739Phe Asp Leu Ser Asp Ala Gln Pro Gly Ser Ala Pro Asp Leu Val Pro
200 205 210ggt ggc tac atg tgg gaa tgc ccc aac ctt ttt acg ctt cgc gat gaa 787Gly Gly Tyr Met Trp Glu Cys Pro Asn Leu Phe Thr Leu Arg Asp Glu
215 220 225gaa act ggc gaa gat ctc gac gtg ctg att ttc tgt cca caa gga ttg 835Glu Thr Gly Glu Asp Leu Asp Val Leu Ile Phe Cys Pro Gln Gly Leu230 235 240 245gac cga atc cac gat gag gtt act cac tac gca agc tct gac cag tgc 883Asp Arg Ile His Asp Glu Val Thr His Tyr Ala Ser Ser Asp Gln Cys
250 255 260gga tat gtc gtc ggc aag ctt gaa gga acg acc ttc cgc gtc ttg cga 931Gly Tyr Val Val Gly Lys Leu Glu Gly Thr Thr Phe Arg Val Leu Arg
265 270 275gga ttc agc gag ctg gat ttc ggc cat gaa ttc tac gca ccg cag gtt 979Gly Phe Ser Glu Leu Asp Phe Gly His Glu Phe Tyr Ala Pro Gln Val
280 285 290gca gta aac ggt tct gat gcc tgg ctc gtg ggc tgg atg ggg ctg ccc 1027Ala Val Asn Gly Ser Asp Ala Trp Leu Val Gly Trp Met Gly Leu Pro
295 300 305gcg cag gat gat cac cca aca gtt gca cgg gaa gga tgg gtg cac tgc 1075Ala Gln Asp Asp His Pro Thr Val Ala Arg Glu Gly Trp Val His Cys310 315 320 325ctg act gtg ccc cgc aag ctt cat ttg cgc aac cac gcg atc tat caa 1123Leu Thr Val Pro Arg Lys Leu His Leu Arg Asn His Ala Ile Tyr Gln
330 335 340gag ctt ctt ctc cca gag ggg gag tca ggg gta atc aga tct gta tta 1171Glu Leu Leu Leu Pro Glu Gly Glu Ser Gly Val Ile Arg Ser Val Leu
345 350 355ggt tct gaa cct gtc cga gta gac atc cga ggc aat att tcc ctc gag 1219Gly Ser Glu Pro Val Arg Val Asp Ile Arg Gly Asn Ile Ser Leu Glu
360 365 370tgg gat ggt gtc cgt ttg tct gtg gat cgt ggt ggt gat cgt cgc gta 1267Trp Asp Gly Val Arg Leu Ser Val Asp Arg Gly Gly Asp Arg Arg Val
375 380 385gct gag gta aaa cct ggc gaa tta gtg atc gcg gac gat aat aca gcc 1315Ala Glu Val Lys Pro Gly Glu Leu Val Ile Ala Asp Asp Asn Thr Ala390 395 400 405att gag ata act gca ggt gat gga cag 1342Ile Glu Ile Thr Ala Gly Asp Gly Gln
410<210>348<211>414<212>PRT<213>谷氨酸棒杆菌<400>348Met His Thr Glu Leu Ser Ser Leu Arg Pro Ala Tyr His Val Thr Pro1 5 10 15Pro Gln Gly Arg Leu Asn Asp Pro Asn Gly Met Tyr Val Asp Gly Asp
20 25 30Thr Leu His Val Tyr Tyr Gln His Asp Pro Gly Phe Pro Phe Ala Pro
35 40 45Lys Arg Thr Gly Trp Ala His Thr Thr Thr Pro Leu Thr Gly Pro Gln
50 55 60Arg Leu Gln Trp Thr His Leu Pro Asp Ala Leu Tyr Pro Asp Ala Ser65 70 75 80Tyr Asp Leu Asp Gly Cys Tyr Ser Gly Gly Ala Val Phe Thr Asp Gly
85 90 95Thr Leu Lys Leu Phe Tyr Thr Gly Asn Leu Lys Ile Asp Gly Lys Arg
100 105 110Arg Ala Thr Gln Asn Leu Val Glu Val Glu Asp Pro Thr Gly Leu Met
115 120 125Gly Gly Ile His Arg Arg Ser Pro Lys Asn Pro Leu Ile Asp Gly Pro
130 135 140Ala Ser Gly Phe Thr Pro His Tyr Arg Asp Pro Met Ile Ser Pro Asp145 150 155 160Gly Asp Gly Trp Lys Met Val Leu Gly Ala Gln Arg Glu Asn Leu Thr
165 170 175Gly Ala Ala Val Leu Tyr Arg Ser Thr Asp Leu Glu Asn Trp Glu Phe
180 185 190Ser Gly Glu Ile Thr Phe Asp Leu Ser Asp Ala Gln Pro Gly Ser Ala
195 200 205Pro Asp Leu Val Pro Gly Gly Tyr Met Trp Glu Cys Pro Asn Leu Phe
210 215 220Thr Leu Arg Asp Glu Glu Thr Gly Glu Asp Leu Asp Val Leu Ile Phe225 230 235 240Cys Pro Gln Gly Leu Asp Arg Ile His Asp Glu Val Thr His Tyr Ala
245 250 255Ser Ser Asp Gln Cys Gly Tyr Val Val Gly Lys Leu Glu Gly Thr Thr
260 265 270Phe Arg Val Leu Arg Gly Phe Ser Glu Leu Asp Phe Gly His Glu Phe
275 280 285Tyr Ala Pro Gln Val Ala Val Asn Gly Ser Asp Ala Trp Leu Val Gly
290 295 300Trp Met Gly Leu Pro Ala Gln Asp Asp His Pro Thr Val Ala Arg Glu305 310 315 320Gly Trp Val His Cys Leu Thr Val Pro Arg Lys Leu His Leu Arg Asn
325 330 335His Ala Ile Tyr Gln Glu Leu Leu Leu Pro Glu Gly Glu Ser Gly Val
340 345 350Ile Arg Ser Val Leu Gly Ser Glu Pro Val Arg Val Asp Ile Arg Gly
355 360 365Asn Ile Ser Leu Glu Trp Asp Gly Val Arg Leu Ser Val Asp Arg Gly
370 375 380Gly Asp Arg Arg Val Ala Glu Val Lys Pro Gly Glu Leu Val Ile Ala385 390 395 400Asp Asp Asn Thr Ala Ile Glu Ile Thr Ala Gly Asp Gly Gln
405 410<210>349<211>720<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(720)<223>RXA02026<400>349cca ttt cat ata caa cca gaa aca ggt tta tta aat gat ccc aac gga 48Pro Phe His Ile Gln Pro Glu Thr Gly Leu Leu Asn Asp Pro Asn Gly1 5 10 15ctt att ttt tat aaa ggg aag tat tat gtt tca cat caa tgg ttc cca 96Leu Ile Phe Tyr Lys Gly Lys Tyr Tyr Val Ser His Gln Trp Phe Pro
20 25 30tta ggc gca gta cat ggc tta aag tat tgg tat aac tac acg agt gat 144Leu Gly Ala Val His Gly Leu Lys Tyr Trp Tyr Asn Tyr Thr Ser Asp
35 40 45gac tta ata aac ttt aaa cct gaa ggg cca ata tta aat cca gat act 192Asp Leu Ile Asn Phe Lys Pro Glu Gly Pro Ile Leu Asn Pro Asp Thr
50 55 60aaa tat gac agc cat ggt gtt tat agc ggt agc gct ttt gaa tat aac 240Lys Tyr Asp Ser His Gly Val Tyr Ser Gly Ser Ala Phe Glu Tyr Asn65 70 75 80ggg cat tta tat tat atg tac aca gga aat cat cga gat aat cat tgg 288Gly His Leu Tyr Tyr Met Tyr Thr Gly Asn His Arg Asp Asn His Trp
85 90 95caa cga cat gcg agt cag atg atc gca cga ttg aaa gaa gac ggt tca 336Gln Arg His Ala Ser Gln Met Ile Ala Arg Leu Lys Glu Asp Gly Ser
100 105 110gtt gaa aag ttt cca aag cca gta att agc cag caa cca gaa gga tat 384Val Glu Lys Phe Pro Lys Pro Val Ile Ser Gln Gln Pro Glu Gly Tyr
115 120 125aca agt cat ttt aga gat cct aaa gtt ttt aaa tat ggt gag aaa tat 432Thr Ser His Phe Arg Asp Pro Lys Val Phe Lys Tyr Gly Glu Lys Tyr
130 135 140tat gca atc att ggc gca caa aat aat gat cag caa ggt cga tta tta 480Tyr Ala Ile Ile Gly Ala Gln Asn Asn Asp Gln Gln Gly Arg Leu Leu145 150 155 160ctt tat aat act gaa gat ata att aat tgg cat tat tta ggt gaa ata 528Leu Tyr Asn Thr Glu Asp Ile Ile Asn Trp His Tyr Leu Gly Glu Ile
165 170 175aat aca gag tta gat gat ttt gga tat atg tgg gaa tgc cca gat tac 576Asn Thr Glu Leu Asp Asp Phe Gly Tyr Met Trp Glu Cys Pro Asp Tyr
180 185 190ttt aat tta gat aat caa gat gtc ata ctt att tgt cca caa ggt att 624Phe Asn Leu Asp Asn Gln Asp Val Ile Leu Ile Cys Pro Gln Gly Ile
195 200 205gaa cca aaa ggc aat cag ttc aaa aat att tat caa agt ggt tat ata 672Glu Pro Lys Gly Asn Gln Phe Lys Asn Ile Tyr Gln Ser Gly Tyr Ile
210 215 220ctt gga aag ttt gat att gaa aag tta aca tat gaa cat gaa aat ttt 720Leu Gly Lys Phe Asp Ile Glu Lys Leu Thr Tyr Glu His Glu Asn Phe225 230 235 240<210>350<211>240<212>PRT<213>谷氨酸棒杆菌<400>350Pro Phe His Ile Gln Pro Glu Thr Gly Leu Leu Asn Asp Pro Asn Gly1 5 10 15Leu Ile Phe Tyr Lys Gly Lys Tyr Tyr Val Ser His Gln Trp Phe Pro
20 25 30Leu Gly Ala Val His Gly Leu Lys Tyr Trp Tyr Asn Tyr Thr Ser Asp
35 40 45Asp Leu Ile Asn Phe Lys Pro Glu Gly Pro Ile Leu Asn Pro Asp Thr
50 55 60Lys Tyr Asp Ser His Gly Val Tyr Ser Gly Ser Ala Phe Glu Tyr Asn65 70 75 80Gly His Leu Tyr Tyr Met Tyr Thr Gly Asn His Arg Asp Asn His Trp
85 90 95Gln Arg His Ala Ser Gln Met Ile Ala Arg Leu Lys Glu Asp Gly Ser
100 105 110Val Glu Lys Phe Pro Lys Pro Val Ile Ser Gln Gln Pro Glu Gly Tyr
115 120 125Thr Ser His Phe Arg Asp Pro Lys Val Phe Lys Tyr Gly Glu Lys Tyr
130 135 140Tyr Ala Ile Ile Gly Ala Gln Asn Asn Asp Gln Gln Gly Arg Leu Leu145 150 155 160Leu Tyr Asn Thr Glu Asp Ile Ile Asn Trp His Tyr Leu Gly Glu Ile
165 170 175Asn Thr Glu Leu Asp Asp Phe Gly Tyr Met Trp Glu Cys Pro Asp Tyr
180 185 190Phe Asn Leu Asp Asn Gln Asp Val Ile Leu Ile Cys Pro Gln Gly Ile
195 200 205Glu Pro Lys Gly Asn Gln Phe Lys Asn Ile Tyr Gln Ser Gly Tyr Ile
210 215 220Leu Gly Lys Phe Asp Ile Glu Lys Leu Thr Tyr Glu His Glu Asn Phe225 230 235 240<210>351<211>1617<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1594)<223>RXA02061<400>351taacctgcct tcacctggga ttatccaccc atgtgtgatg aaatcgctac cctgaatcaa 60agacactggc gtaattgagt gaaggcagga caataaagag atg acg aac gtt tcc 115
Met Thr Asn Val Ser
1 5ggg tat cac cga cca gag ctg cac atc acc gct gaa agt ggt gtt ttg 163Gly Tyr His Arg Pro Glu Leu His Ile Thr Ala Glu Ser Gly Val Leu
10 15 20ttt gca cct gca ggc gtt ctg ttg gat gac gac acg tgg cat ttc ttc 211Phe Ala Pro Ala Gly Val Leu Leu Asp Asp Asp Thr Trp His Phe Phe
25 30 35cac cag tac cgt ccc tca cca gat cac ggc ccc agg tgg gcg cac caa 259His Gln Tyr Arg Pro Ser Pro Asp His Gly Pro Arg Trp Ala His Gln
40 45 50ttc gca gag cgc act cca ttt gtg tgg gat atc tgc gat gac gtg cta 307Phe Ala Glu Arg Thr Pro Phe Val Trp Asp Ile Cys Asp Asp Val Leu
55 60 65gcc cct gaa ggc gat gaa acc cag gtt cgc gct ggc tca gtg gtg tcc 355Ala Pro Glu Gly Asp Glu Thr Gln Val Arg Ala Gly Ser Val Val Ser70 75 80 85aac aac ggt ggc gtt gat ctg tac ttc acc tcg gtt gtt ggc ccc act 403Asn Asn Gly Gly Val Asp Leu Tyr Phe Thr Ser Val Val Gly Pro Thr
90 95 100tcc act atc cag ttg gca cac atc aac aac atc cgt ggc acc acc gaa 451Ser Thr Ile Gln Leu Ala His Ile Asn Asn Ile Arg Gly Thr Thr Glu
105 110 115ctg atc aat gag gac gag ctg ggg ctc gat cca gat gtc tcc cga atc 499Leu Ile Asn Glu Asp Glu Leu Gly Leu Asp Pro Asp Val Ser Arg Ile
120 125 130ggc gaa gtg gtt ggc aac act gat ggt tat gta aag ttc cgc tca ccg 547Gly Glu Val Val Gly Asn Thr Asp Gly Tyr Val Lys Phe Arg Ser Pro
135 140 145tgc gtt atc cca ggt tgg gaa gac caa gga aac cgc gat gaa ggc cac 595Cys Val Ile Pro Gly Trp Glu Asp Gln Gly Asn Arg Asp Glu Gly His150 155 160 165tca gga tgg ttg atg ctc gca gtt act ggc cca gtt gaa gcc cca aca 643Ser Gly Trp Leu Met Leu Ala Val Thr Gly Pro Val Glu Ala Pro Thr
170 175 180gta gtg gtc ctc gac tcg cca gat gga aga gaa tgg tcc att aca ggt 691Val Val Val Leu Asp Ser Pro Asp Gly Arg Glu Trp Ser Ile Thr Gly
185 190 195ccc ctg tct ctc aac ggc ctc tct gga tta gag tca gac gaa gtt cta 739Pro Leu Ser Leu Asn Gly Leu Ser Gly Leu Glu Ser Asp Glu Val Leu
200 205 210gtt gct cct cgc atg att cgt ctg cgc gat gaa gtg gat cat gaa atc 787Val Ala Pro Arg Met Ile Arg Leu Arg Asp Glu Val Asp His Glu Ile
215 220 225tac gat gtc ctc att gtc acc att gaa caa gac ggg att gac att tcg 835Tyr Asp Val Leu Ile Val Thr Ile Glu Gln Asp Gly Ile Asp Ile Ser230 235 240 245gga tac ctg gta ggc cag ctc aac ggc tca gaa ttc gat gtg aag act 883Gly Tyr Leu Val Gly Gln Leu Asn Gly Ser Glu Phe Asp Val Lys Thr
250 255 260cca ttt acc cgc atc gat ttt ggc cat gat ttc tct cgc ccc cgc aac 931Pro Phe Thr Arg Ile Asp Phe Gly His Asp Phe Ser Arg Pro Arg Asn
265 270 275acc aac tac gcc gaa acc acc atc ggc tac gac ttc gcc cac atc ttt 979Thr Asn Tyr Ala Glu Thr Thr Ile Gly Tyr Asp Phe Ala His Ile Phe
280 285 290ggt ctc atg aat ggc gta ggt cgt ttg gac tcc ccc act gag cat ctc 1027Gly Leu Met Asn Gly Val Gly Arg Leu Asp Ser Pro Thr Glu His Leu
295 300 305agt tgg aag gaa gaa ggc tgg gca aac gct att tct ttc cca cgt att 1075Ser Trp Lys Glu Glu Gly Trp Ala Asn Ala Ile Ser Phe Pro Arg Ile310 315 320 325gtc acg ctc cag gac ggt acg gtc ttc cag acc cct cca gaa gga ttg 1123Val Thr Leu Gln Asp Gly Thr Val Phe Gln Thr Pro Pro Glu Gly Leu
330 335 340ctt gat gcc att cat gaa tcc gag gca gcg gca ggt tgg acc gga ctg 1171Leu Asp Ala Ile His Glu Ser Glu Ala Ala Ala Gly Trp Thr Gly Leu
345 350 355tgc gaa atc cca tca aac agc gca gtt gaa gtg gcg ttg aag gac caa 1219Cys Glu Ile Pro Ser Asn Ser Ala Val Glu Val Ala Leu Lys Asp Gln
360 365 370gaa ggt gaa atc gct gca aca atc act cac cgc cac aat cag cta gtc 1267Glu Gly Glu Ile Ala Ala Thr Ile Thr His Arg His Asn Gln Leu Val
375 380 385gtt gat cgg tcc atg aac ccc aac cac gcg ggt gat cca cac gcg att 1315Val Asp Arg Ser Met Asn Pro Asn His Ala Gly Asp Pro His Ala Ile390 395 400 405gca cca ttg act gat gat gaa aca gat tca ctg ttc att gtc gtt gac 1363Ala Pro Leu Thr Asp Asp Glu Thr Asp Ser Leu Phe Ile Val Val Asp
410 415 420ggc tct aca gta gaa gtt ttt gct gat ggc ggt tat gta tca atg gca 1411Gly Ser Thr Val Glu Val Phe Ala Asp Gly Gly Tyr Val Ser Met Ala
425 430 435agc cgt gtg tat ttc aac aac gga cca ttc agc gaa ttt gag gtc acc 1459Ser Arg Val Tyr Phe Asn Asn Gly Pro Phe Ser Glu Phe Glu Val Thr
440 445 450acc acc ggt gac gca agc att att cgc cag gaa agt cac ttc cct gtt 1507Thr Thr Gly Asp Ala Ser Ile Ile Arg Gln Glu Ser His Phe Pro Val
455 460 465gat ttc agt tcg gtg tcc cta gat ata gat gat ctc act gcg ctc atg 1555Asp Phe Ser Ser Val Ser Leu Asp Ile Asp Asp Leu Thr Ala Leu Met470 475 480 485cag ttc gat gaa aac gaa ccg cat gaa ggc cca gtg aga taagagttag 1604Gln Phe Asp Glu Asn Glu Pro His Glu Gly Pro Val Arg
490 495atgcgttcca gcc 1617<210>352<211>498<212>PRT<213>谷氨酸棒杆菌<400>352Met Thr Asn Val Ser Gly Tyr His Arg Pro Glu Leu His Ile Thr Ala1 5 10 15Glu Ser Gly Val Leu Phe Ala Pro Ala Gly Val Leu Leu Asp Asp Asp
20 25 30Thr Trp His Phe Phe His Gln Tyr Arg Pro Ser Pro Asp His Gly Pro
35 40 45Arg Trp Ala His Gln Phe Ala Glu Arg Thr Pro Phe Val Trp Asp Ile
50 55 60Cys Asp Asp Val Leu Ala Pro Glu Gly Asp Glu Thr Gln Val Arg Ala65 70 75 80Gly Ser Val Val Ser Asn Asn Gly Gly Val Asp Leu Tyr Phe Thr Ser
85 90 95Val Val Gly Pro Thr Ser Thr Ile Gln Leu Ala His Ile Asn Asn Ile
100 105 110Arg Gly Thr Thr Glu Leu Ile Asn Glu Asp Glu Leu Gly Leu Asp Pro
115 120 125Asp Val Ser Arg Ile Gly Glu Val Val Gly Asn Thr Asp Gly Tyr Val
130 135 140Lys Phe Arg Ser Pro Cys Val Ile Pro Gly Trp Glu Asp Gln Gly Asn145 150 155 160Arg Asp Glu Gly His Ser Gly Trp Leu Met Leu Ala Val Thr Gly Pro
165 170 175Val Glu Ala Pro Thr Val Val Val Leu Asp Ser Pro Asp Gly Arg Glu
180 185 190Trp Ser Ile Thr Gly Pro Leu Ser Leu Asn Gly Leu Ser Gly Leu Glu
195 200 205Ser Asp Glu Val Leu Val Ala Pro Arg Met Ile Arg Leu Arg Asp Glu
210 215 220Val Asp His Glu Ile Tyr Asp Val Leu Ile Val Thr Ile Glu Gln Asp225 230 235 240Gly Ile Asp Ile Ser Gly Tyr Leu Val Gly Gln Leu Asn Gly Ser Glu
245 250 255Phe Asp Val Lys Thr Pro Phe Thr Arg Ile Asp Phe Gly His Asp Phe
260 265 270Ser Arg Pro Arg Asn Thr Asn Tyr Ala Glu Thr Thr Ile Gly Tyr Asp
275 280 285Phe Ala His Ile Phe Gly Leu Met Asn Gly Val Gly Arg Leu Asp Ser
290 295 300Pro Thr Glu His Leu Ser Trp Lys Glu Glu Gly Trp Ala Asn Ala Ile305 310 315 320Ser Phe Pro Arg Ile Val Thr Leu Gln Asp Gly Thr Val Phe Gln Thr
325 330 335Pro Pro Glu Gly Leu Leu Asp Ala Ile His Glu Ser Glu Ala Ala Ala
340 345 350Gly Trp Thr Gly Leu Cys Glu Ile Pro Ser Asn Ser Ala Val Glu Val
355 360 365Ala Leu Lys Asp Gln Glu Gly Glu Ile Ala Ala Thr Ile Thr His Arg
370 375 380His Asn Gln Leu Val Val Asp Arg Ser Met Asn Pro Asn His Ala Gly385 390 395 400Asp Pro His Ala Ile Ala Pro Leu Thr Asp Asp Glu Thr Asp Ser Leu
405 410 415Phe Ile Val Val Asp Gly Ser Thr Val Glu Val Phe Ala Asp Gly Gly
420 425 430Tyr Val Ser Met Ala Ser Arg Val Tyr Phe Asn Asn Gly Pro Phe Ser
435 440 445Glu Phe Glu Val Thr Thr Thr Gly Asp Ala Ser Ile Ile Arg Gln Glu
450 455 460Ser His Phe Pro Val Asp Phe Ser Ser Val Ser Leu Asp Ile Asp Asp465 470 475 480Leu Thr Ala Leu Met Gln Phe Asp Glu Asn Glu Pro His Glu Gly Pro
485 490 495Val Arg<210>353<211>1305<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1282)<223>RXN01369<400>353ctgcgacgga cctagcaaag gggcgctgac acaagcactg cgtttgctgg tgcgcggaca 60gtcagccacg acctattcca ttgaagaaaa ggacttgtaa atg gag cta ttg gaa 115
Met Glu Leu Leu Glu
1 5ggc tca ctg cgc acc tac cca tgg ggt tca aga aca ctg atc gct gat 163Gly Ser Leu Arg Thr Tyr Pro Trp Gly Ser Arg Thr Leu Ile Ala Asp
10 15 20ctc aaa ggc gaa gaa tca cca tcg tct cgc cca gag gcc gaa gtc tgg 211Leu Lys Gly Glu Glu Ser Pro Ser Ser Arg Pro Glu Ala Glu Val Trp
25 30 35ttc ggt gcc cac cca gga tca cca tca acc atc ggt gga aac gca ctc 259Phe Gly Ala His Pro Gly Ser Pro Ser Thr Ile Gly Gly Asn Ala Leu
40 45 50aac gaa gtc atc gca gcg aac ccc gaa gaa gca ttg ggc acg cgt gtt 307Asn Glu Val Ile Ala Ala Asn Pro Glu Glu Ala Leu Gly Thr Arg Val
55 60 65gcc gaa gcg ttt gaa aat gag ctt cca ttc ctc ctc aaa atc ctc gca 355Ala Glu Ala Phe Glu Asn Glu Leu Pro Phe Leu Leu Lys Ile Leu Ala70 75 80 85gcg gga gca ccc cta tca ctg cag gcc cac cca tcg ctg gaa cag gcc 403Ala Gly Ala Pro Leu Ser Leu Gln Ala His Pro Ser Leu Glu Gln Ala
90 95 100cgt gaa gga ttc gcc cgc gaa aac tca gca gga att gac ctc ggc gca 451Arg Glu Gly Phe Ala Arg Glu Asn Ser Ala Gly Ile Asp Leu Gly Ala
105 110 115ccg aac cgc aac tac cgc gac cca aac cac aag cca gag ctg atc gtt 499Pro Asn Arg Asn Tyr Arg Asp Pro Asn His Lys Pro Glu Leu Ile Val
120 125 130gct ctc acg gaa ttc atc gcg atg gca ggc ttc cgc cca ctg cgg aac 547Ala Leu Thr Glu Phe Ile Ala Met Ala Gly Phe Arg Pro Leu Arg Asn
135 140 145acc ctc acc att ttc gac gcc ctc gcc tgc gaa ccc ctc gac cgc tac 595Thr Leu Thr Ile Phe Asp Ala Leu Ala Cys Glu Pro Leu Asp Arg Tyr150 155 160 165cgc agc atg ctc acc gtc gac aac gag gaa gaa tcc ctc cgc gca ctg 643Arg Ser Met Leu Thr Val Asp Asn Glu Glu Glu Ser Leu Arg Ala Leu
170 175 180ttt acc acc tgg atc acc atc ccc atc ggt aaa cga cac gaa ctc atc 691Phe Thr Thr Trp Ile Thr Ile Pro Ile Gly Lys Arg His Glu Leu Ile
185 190 195gat gcc ctc atc agc aac gcc cac acc tac ctt gag gca agc gat cgt 739Asp Ala Leu Ile Ser Asn Ala His Thr Tyr Leu Glu Ala Ser Asp Arg
200 205 210gac gag gac atc gca ttc gtg ctc tca cac atc atc gag ctc aac gaa 787Asp Glu Asp Ile Ala Phe Val Leu Ser His Ile Ile Glu Leu Asn Glu
215 220 225cag tac ccc ggc gat gtc ggc gtt ctg ggt gct ctg ctg ttg aac ttc 835Gln Tyr Pro Gly Asp Val Gly Val Leu Gly Ala Leu Leu Leu Asn Phe230 235 240 245tac aaa ctt gcc cca ggc gaa gcc ctc tac ctc gac gcc gca aac ctt 883Tyr Lys Leu Ala Pro Gly Glu Ala Leu Tyr Leu Asp Ala Ala Asn Leu
250 255 260cac gca tac atc agc ggc ctc ggc gta gag atc atg gcg aac tcc gac 931His Ala Tyr Ile Ser Gly Leu Gly Val Glu Ile Met Ala Asn Ser Asp
265 270 275aac gtg ctc cgc ggt gga ctg aca tcc aaa tac gtc gac gtc ccg gag 979Asn Val Leu Arg Gly Gly Leu Thr Ser Lys Tyr Val Asp Val Pro Glu
280 285 290ctt gtg cgc gtg ttg gat ttc aac tct ttg gaa aac gct cgc gtg gac 1027Leu Val Arg Val Leu Asp Phe Asn Ser Leu Glu Asn Ala Arg Val Asp
295 300 305gtt gaa gaa gac ggt gca acg acc cac tac cca gtt cca atc aac gaa 1075Val Glu Glu Asp Gly Ala Thr Thr His Tyr Pro Val Pro Ile Asn Glu310 315 320 325ttc caa ctc gat cgc gtt gca gtt cag ggc gaa gca gaa gcc aac cac 1123Phe Gln Leu Asp Arg Val Ala Val Gln Gly Glu Ala Glu Ala Asn His
330 335 340gat ggt ccc atg att gtt ctg tgc acc tcc gga act gtt tcc ttg gaa 1171Asp Gly Pro Met Ile Val Leu Cys Thr Ser Gly Thr Val Ser Leu Glu
345 350 355gca ggg gag aag acc ctc gaa gta gca gca ggt cac gcc gca tgg gtt 1219Ala Gly Glu Lys Thr Leu Glu Val Ala Ala Gly His Ala Ala Trp Val
360 365 370cca gca aac gac cca acc att gcg atg cgt tct gag gac gca gaa gta 1267Pro Ala Asn Asp Pro Thr Ile Ala Met Arg Ser Glu Asp Ala Glu Val
375 380 385ttc ctc gct agg gtt tagatctttt tagattaaaa tca 1305Phe Leu Ala Arg Val390<210>354<211>394<212>PRT<213>谷氨酸棒杆菌<400>354Met Glu Leu Leu Glu Gly Ser Leu Arg Thr Tyr Pro Trp Gly Ser Arg1 5 10 15Thr Leu Ile Ala Asp Leu Lys Gly Glu Glu Ser Pro Ser Ser Arg Pro
20 25 30Glu Ala Glu Val Trp Phe Gly Ala His Pro Gly Ser Pro Ser Thr Ile
35 40 45Gly Gly Asn Ala Leu Asn Glu Val Ile Ala Ala Asn Pro Glu Glu Ala
50 55 60Leu Gly Thr Arg Val Ala Glu Ala Phe Glu Asn Glu Leu Pro Phe Leu65 70 75 80Leu Lys Ile Leu Ala Ala Gly Ala Pro Leu Ser Leu Gln Ala His Pro
85 90 95Ser Leu Glu Gln Ala Arg Glu Gly Phe Ala Arg Glu Asn Ser Ala Gly
100 105 110Ile Asp Leu Gly Ala Pro Asn Arg Asn Tyr Arg Asp Pro Asn His Lys
115 120 125Pro Glu Leu Ile Val Ala Leu Thr Glu Phe Ile Ala Met Ala Gly Phe
130 135 140Arg Pro Leu Arg Asn Thr Leu Thr Ile Phe Asp Ala Leu Ala Cys Glu145 150 155 160Pro Leu Asp Arg Tyr Arg Ser Met Leu Thr Val Asp Asn Glu Glu Glu
165 170 175Ser Leu Arg Ala Leu Phe Thr Thr Trp Ile Thr Ile Pro Ile Gly Lys
180 185 190Arg His Glu Leu Ile Asp Ala Leu Ile Ser Asn Ala His Thr Tyr Leu
195 200 205Glu Ala Ser Asp Arg Asp Glu Asp Ile Ala Phe Val Leu Ser His Ile
210 215 220Ile Glu Leu Asn Glu Gln Tyr Pro Gly Asp Val Gly Val Leu Gly Ala225 230 235 240Leu Leu Leu Asn Phe Tyr Lys Leu Ala Pro Gly Glu Ala Leu Tyr Leu
245 250 255Asp Ala Ala Asn Leu His Ala Tyr Ile Ser Gly Leu Gly Val Glu Ile
260 265 270Met Ala Asn Ser Asp Asn Val Leu Arg Gly Gly Leu Thr Ser Lys Tyr
275 280 285Val Asp Val Pro Glu Leu Val Arg Val Leu Asp Phe Asn Ser Leu Glu
290 295 300Asn Ala Arg Val Asp Val Glu Glu Asp Gly Ala Thr Thr His Tyr Pro305 310 315 320Val Pro Ile Asn Glu Phe Gln Leu Asp Arg Val Ala Val Gln Gly Glu
325 330 335Ala Glu Ala Asn His Asp Gly Pro Met Ile Val Leu Cys Thr Ser Gly
340 345 350Thr Val Ser Leu Glu Ala Gly Glu Lys Thr Leu Glu Val Ala Ala Gly
355 360 365His Ala Ala Trp Val Pro Ala Asn Asp Pro Thr Ile Ala Met Arg Ser
370 375 380Glu Asp Ala Glu Val Phe Leu Ala Arg Val385 390<210>355<211>524<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(501)<223>FRXA01369<400>355aac gaa cag tgc ctc ggc gat gtc ggc gtt ctg ggt gct ctg ctg ttg 48Asn Glu Gln Cys Leu Gly Asp Val Gly Val Leu Gly Ala Leu Leu Leu1 5 10 15aac ttc tac aaa ctt gcc cca ggc gaa gcc ctc tac ctc gac gcc gca 96Asn Phe Tyr Lys Leu Ala Pro Gly Glu Ala Leu Tyr Leu Asp Ala Ala
20 25 30aac ctt cac gca tac atc agc ggc ctc ggc gta gag atc atg gcg aac 144Asn Leu His Ala Tyr Ile Ser Gly Leu Gly Val Glu Ile Met Ala Asn
35 40 45tcc gac aac gtg ctc cgc ggt gga ctg aca tcc aaa tac gtc gac gtc 192Ser Asp Asn Val Leu Arg Gly Gly Leu Thr Ser Lys Tyr Val Asp Val
50 55 60ccg gag ctt gtg cgc gtg ttg gat ttc aac tct ttg gaa aac gct cgc 240Pro Glu Leu Val Arg Val Leu Asp Phe Asn Ser Leu Glu Asn Ala Arg65 70 75 80gtg gac gtt gaa gaa gac ggt gca acg acc cac tac cca gtt cca atc 288Val Asp Val Glu Glu Asp Gly Ala Thr Thr His Tyr Pro Val Pro Ile
85 90 95aac gaa ttc caa ctc gat cgc gtt gca gtt cag ggc gaa gca gaa gcc 336Asn Glu Phe Gln Leu Asp Arg Val Ala Val Gln Gly Glu Ala Glu Ala
100 105 110aac cac gat ggt ccc atg att gtt ctg tgc acc tcc gga act gtt tcc 384Asn His Asp Gly Pro Met Ile Val Leu Cys Thr Ser Gly Thr Val Ser
115 120 125ttg gaa gca ggg gag aag acc ctc gaa gta gca gca ggt cac gcc gca 432Leu Glu Ala Gly Glu Lys Thr Leu Glu Val Ala Ala Gly His Ala Ala
130 135 140tgg gtt cca gca aac gac cca acc att gcg atg cgt tct gag gac gca 480Trp Val Pro Ala Asn Asp Pro Thr Ile Ala Met Arg Ser Glu Asp Ala145 150 155 160gaa gta ttc ctc gct agg gtt tagatctttt tagattaaaa tca 524Glu Val Phe Leu Ala Arg Val
165<210>356<211>167<212>PRT<213>谷氨酸棒杆菌<400>356Asn Glu Gln Cys Leu Gly Asp Val Gly Val Leu Gly Ala Leu Leu Leu1 5 10 15Asn Phe Tyr Lys Leu Ala Pro Gly Glu Ala Leu Tyr Leu Asp Ala Ala
20 25 30Asn Leu His Ala Tyr Ile Ser Gly Leu Gly Val Glu Ile Met Ala Asn
35 40 45Ser Asp Asn Val Leu Arg Gly Gly Leu Thr Ser Lys Tyr Val Asp Val
50 55 60Pro Glu Leu Val Arg Val Leu Asp Phe Asn Ser Leu Glu Asn Ala Arg65 70 75 80Val Asp Val Glu Glu Asp Gly Ala Thr Thr His Tyr Pro Val Pro Ile
85 90 95Asn Glu Phe Gln Leu Asp Arg Val Ala Val Gln Gly Glu Ala Glu Ala
100 105 110Asn His Asp Gly Pro Met Ile Val Leu Cys Thr Ser Gly Thr Val Ser
115 120 125Leu Glu Ala Gly Glu Lys Thr Leu Glu Val Ala Ala Gly His Ala Ala
130 135 140Trp Val Pro Ala Asn Asp Pro Thr Ile Ala Met Arg Ser Glu Asp Ala145 150 155 160Glu Val Phe Leu Ala Arg Val
165<210>357<211>808<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(808)<223>FRXA01373<400>357ctgcgacgga cctagcaaag gggcgctgac acaagcactg cgtttgctgg tgcgcggaca 60gtcagccacg acctattcca ttgaagaaaa ggacttgtaa atg gag cta ttg gaa 115
Met Glu Leu Leu Glu
1 5ggc tca ctg cgc acc tac cca tgg ggt tca aga aca ctg atc gct gat 163Gly Ser Leu Arg Thr Tyr Pro Trp Gly Ser Arg Thr Leu Ile Ala Asp
10 15 20ctc aaa ggc gaa gaa tca cca tcg tct cgc cca gag gcc gaa gtc tgg 211Leu Lys Gly Glu Glu Ser Pro Ser Ser Arg Pro Glu Ala Glu Val Trp
25 30 35ttc ggt gcc cac cca gga tca cca tca acc atc ggt gga aac gca ctc 259Phe Gly Ala His Pro Gly Ser Pro Ser Thr Ile Gly Gly Asn Ala Leu
40 45 50aac gaa gtc atc gca gcg aac ccc gaa gaa gca ttg ggc acg cgt gtt 307Asn Glu Val Ile Ala Ala Asn Pro Glu Glu Ala Leu Gly Thr Arg Val
55 60 65gcc gaa gcg ttt gaa aat gag ctt cca ttc ctc ctc aaa atc ctc gca 355Ala Glu Ala Phe Glu Asn Glu Leu Pro Phe Leu Leu Lys Ile Leu Ala70 75 80 85gcg gga gca ccc cta tca ctg cag gcc cac cca tcg ctg gaa cag gcc 403Ala Gly Ala Pro Leu Ser Leu Gln Ala His Pro Ser Leu Glu Gln Ala
90 95 100cgt gaa gga ttc gcc cgc gaa aac tca gca gga att gac ctc ggc gca 451Arg Glu Gly Phe Ala Arg Glu Asn Ser Ala Gly Ile Asp Leu Gly Ala
105 110 115ccg aac cgc aac tac cgc gac cca aac cac aag cca gag ctg atc gtt 499Pro Asn Arg Asn Tyr Arg Asp Pro Asn His Lys Pro Glu Leu Ile Val
120 125 130gct ctc acg gaa ttc atc gcg atg gca ggc ttc cgc cca ctg cgg aac 547Ala Leu Thr Glu Phe Ile Ala Met Ala Gly Phe Arg Pro Leu Arg Asn
135 140 145acc ctc acc att ttc gac gcc ctc gcc tgc gaa ccc ctc gac cgc tac 595Thr Leu Thr Ile Phe Asp Ala Leu Ala Cys Glu Pro Leu Asp Arg Tyr150 155 160 165cgc agc atg ctc acc gtc gac aac gag gaa gaa tcc ctc cgc gca ctg 643Arg Ser Met Leu Thr Val Asp Asn Glu Glu Glu Ser Leu Arg Ala Leu
170 175 180ttt acc acc tgg atc acc atc ccc atc ggt aaa cga cac gaa ctc atc 691Phe Thr Thr Trp Ile Thr Ile Pro Ile Gly Lys Arg His Glu Leu Ile
185 190 195gat gcc ctc atc agc aac gcc cac acc tac ctt gag gca agc gat cgt 739Asp Ala Leu Ile Ser Asn Ala His Thr Tyr Leu Glu Ala Ser Asp Arg
200 205 210gac gag gac atc gca ttc gtg ctc tca cac atc atc gag ctc aac gaa 787Asp Glu Asp Ile Ala Phe Val Leu Ser His Ile Ile Glu Leu Asn Glu
215 220 225cag tac ccc ggc gat gtc ggc 808Gln Tyr Pro Gly Asp Val Gly230 235<210>358<211>236<212>PRT<213>谷氨酸棒杆菌<400>358Met Glu Leu Leu Glu Gly Ser Leu Arg Thr Tyr Pro Trp Gly Ser Arg1 5 10 15Thr Leu Ile Ala Asp Leu Lys Gly Glu Glu Ser Pro Ser Ser Arg Pro
20 25 30Glu Ala Glu Val Trp Phe Gly Ala His Pro Gly Ser Pro Ser Thr Ile
35 40 45Gly Gly Asn Ala Leu Asn Glu Val Ile Ala Ala Asn Pro Glu Glu Ala
50 55 60Leu Gly Thr Arg Val Ala Glu Ala Phe Glu Asn Glu Leu Pro Phe Leu65 70 75 80Leu Lys Ile Leu Ala Ala Gly Ala Pro Leu Ser Leu Gln Ala His Pro
85 90 95Ser Leu Glu Gln Ala Arg Glu Gly Phe Ala Arg Glu Asn Ser Ala Gly
100 105 110Ile Asp Leu Gly Ala Pro Asn Arg Asn Tyr Arg Asp Pro Asn His Lys
115 120 125Pro Glu Leu Ile Val Ala Leu Thr Glu Phe Ile Ala Met Ala Gly Phe
130 135 140Arg Pro Leu Arg Asn Thr Leu Thr Ile Phe Asp Ala Leu Ala Cys Glu145 150 155 160Pro Leu Asp Arg Tyr Arg Ser Met Leu Thr Val Asp Asn Glu Glu Glu
165 170 175Ser Leu Arg Ala Leu Phe Thr Thr Trp Ile Thr Ile Pro Ile Gly Lys
180 185 190Arg His Glu Leu Ile Asp Ala Leu Ile Ser Asn Ala His Thr Tyr Leu
195 200 205Glu Ala Ser Asp Arg Asp Glu Asp Ile Ala Phe Val Leu Ser His Ile
210 215 220Ile Glu Leu Asn Glu Gln Tyr Pro Gly Asp Val Gly225 230 235<210>359<211>1775<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1752)<223>RXA02611<400>359gat gcg tgg tcg gat cct atg gct acg tgg cgt cat gcg att acc act 48Asp Ala Trp Ser Asp Pro Met Ala Thr Trp Arg His Ala Ile Thr Thr1 5 10 15aag att gag gcc ggc cag ggt tcg gat gag ttg tat aac gac ttt gag 96Lys Ile Glu Ala Gly Gln Gly Ser Asp Glu Leu Tyr Asn Asp Phe Glu
20 25 30cac ggg gct cag ctg ttt gag cgt gct gcg gag aat ttg tct aaa gag 144His Gly Ala Gln Leu Phe Glu Arg Ala Ala Glu Asn Leu Ser Lys Glu
35 40 45gat agg act gcg ctt ttc gac gtc gcc tcc tct ctg cgg cgc ggc ggc 192Asp Arg Thr Ala Leu Phe Asp Val Ala Ser Ser Leu Arg Arg Gly Gly
50 55 60gat gta cgc gca cgt ctc gcc cca gcg ctc acc gcg agt gtc act cat 240Asp Val Arg Ala Arg Leu Ala Pro Ala Leu Thr Ala Ser Val Thr His65 70 75 80ctt tta gaa ctt aac ccg ttg cgt gag ttg gtc acg atg ggt gaa aac 288Leu Leu Glu Leu Asn Pro Leu Arg Glu Leu Val Thr Met Gly Glu Asn
85 90 95ctg cag gtt cgt gtc gag cgt cgt gcc gct ttg gtc aac tct tgg tat 336Leu Gln Val Arg Val Glu Arg Arg Ala Ala Leu Val Asn Ser Trp Tyr
100 105 110gag ctt ttc cct cgt tcc aca ggt ggt tgg gat gag tcc ggc acc ccc 384Glu Leu Phe Pro Arg Ser Thr Gly Gly Trp Asp Glu Ser Gly Thr Pro
115 120 125gtt cat ggc act ttc gct acc act gct cag gcg ttg gag cgt gtc gcg 432Val His Gly Thr Phe Ala Thr Thr Ala Gln Ala Leu Glu Arg Val Ala
130 135 140aag atg ggc ttc gat act gtt tac ttc ccg ccg atc cat ccg att ggc 480Lys Met Gly Phe Asp Thr Val Tyr Phe Pro Pro Ile His Pro Ile Gly145 150 155 160gag gtc aac cgc aag ggc cgc aat aat acg ctg acc ccg gaa cct cat 528Glu Val Asn Arg Lys Gly Arg Asn Asn Thr Leu Thr Pro Glu Pro His
165 170 175gat gtg ggt tcg ccg tgg gct att ggt tct aaa gat ggt ggg cat gat 576Asp Val Gly Ser Pro Trp Ala Ile Gly Ser Lys Asp Gly Gly His Asp
180 185 190gca acg cat ccg cgg ttg ggc acc att gaa gat ttc cag gcg ttg ttg 624Ala Thr His Pro Arg Leu Gly Thr Ile Glu Asp Phe Gln Ala Leu Leu
195 200 205gct cgc gca cgg gaa ctc aat ttg gaa gtt gca ctc gat cta gct ctg 672Ala Arg Ala Arg Glu Leu Asn Leu Glu Val Ala Leu Asp Leu Ala Leu
210 215 220cag gct gcc cct gat cat cca tgg gcg cag gaa cac cgc gag ttt ttc 720Gln Ala Ala Pro Asp His Pro Trp Ala Gln Glu His Arg Glu Phe Phe225 230 235 240acg gtg ttg gct gat ggc acc att gcg tat gca gaa aac cca ccg aag 768Thr Val Leu Ala Asp Gly Thr Ile Ala Tyr Ala Glu Asn Pro Pro Lys
245 250 255aag tac cag gat att tat ccc atc aac ttt gat aat gat gct ccg aag 816Lys Tyr Gln Asp Ile Tyr Pro Ile Asn Phe Asp Asn Asp Ala Pro Lys
260 265 270atc tac gaa gag gtc tat cgt gtg gtg aag ttc tgg gtg gat ttg ggt 864Ile Tyr Glu Glu Val Tyr Arg Val Val Lys Phe Trp Val Asp Leu Gly
275 280 285gtg acc aca ttc cgc gtg gat aac ccg cac act aag ccc gct aat ttc 912Val Thr Thr Phe Arg Val Asp Asn Pro His Thr Lys Pro Ala Asn Phe
290 295 300tgg cag tgg ctt att tct gcc atc cat aaa tca aac cct gag gtc att 960Trp Gln Trp Leu Ile Ser Ala Ile His Lys Ser Asn Pro Glu Val Ile305 310 315 320ttc cta gcg gag gcg tct act cgc ccg gca cgt ctg tat ttc ttg tcc 1008Phe Leu Ala Glu Ala Ser Thr Arg Pro Ala Arg Leu Tyr Phe Leu Ser
325 330 335aag att ggt ttc tcc cag tct tac acc tac ttc acc tgg aag gtc acc 1056Lys Ile Gly Phe Ser Gln Ser Tyr Thr Tyr Phe Thr Trp Lys Val Thr
340 345 350aac gag gag ctc acc gag ttc gct act gag atc gcc ccc atg gcg gat 1104Asn Glu Glu Leu Thr Glu Phe Ala Thr Glu Ile Ala Pro Met Ala Asp
355 360 365att tct cgt ccg aac ctg ttt gtg aac act ccc gac att ttg cat gcg 1152Ile Ser Arg Pro Asn Leu Phe Val Asn Thr Pro Asp Ile Leu His Ala
370 375 380tct ctg cag cat ggt gga cgc gcc atg ttc gct atc cgc gcc gca ttg 1200Ser Leu Gln His Gly Gly Arg Ala Met Phe Ala Ile Arg Ala Ala Leu385 390 395 400gcc gcc acg atg tct cct gtg tgg ggc gta tat tcc gga tat gag ctc 1248Ala Ala Thr Met Ser Pro Val Trp Gly Val Tyr Ser Gly Tyr Glu Leu
405 410 415ttt gag cac gag gcc gtc aag cct ggt tcg gaa gag tac ttg gat tct 1296Phe Glu His Glu Ala Val Lys Pro Gly Ser Glu Glu Tyr Leu Asp Ser
420 425 430gag aag tac gag ctg cgt ccc cgc gat ttc gag ggt gct ctg gaa cgt 1344Glu Lys Tyr Glu Leu Arg Pro Arg Asp Phe Glu Gly Ala Leu Glu Arg
435 440 445ggc gat tct ctc gag gat tac atc gct ctg ctc aac cag atc cgt cgc 1392Gly Asp Ser Leu Glu Asp Tyr Ile Ala Leu Leu Asn Gln Ile Arg Arg
450 455 460gcg aac cct gcc ttg cag caa cta cgc aac atc cac ttc cac gaa gcg 1440Ala Asn Pro Ala Leu Gln Gln Leu Arg Asn Ile His Phe His Glu Ala465 470 475 480gac aat gat cag atc atc gcc tac tcc aag gtt gat gct ttg acc gga 1488Asp Asn Asp Gln Ile Ile Ala Tyr Ser Lys Val Asp Ala Leu Thr Gly
485 490 495aat acc gtg ttg att gtg gtc aac ttg gat cca cgt agt gct cgt gag 1536Asn Thr Val Leu Ile Val Val Asn Leu Asp Pro Arg Ser Ala Arg Glu
500 505 510gct act gtt cgc ctt gat ctt gga gcg ctt ggc tta gaa gcg ggt gca 1584Ala Thr Val Arg Leu Asp Leu Gly Ala Leu Gly Leu Glu Ala Gly Ala
515 520 525cag ttt gag gtg cgc gat gcg atc acc ggc tcc cgt tac ctg tgg tca 1632Gln Phe Glu Val Arg Asp Ala Ile Thr Gly Ser Arg Tyr Leu Trp Ser
530 535 540gag acg aac ttt gtc cgc ctc gag ccc cta cgc gat gtc gcc cac atc 1680Glu Thr Asn Phe Val Arg Leu Glu Pro Leu Arg Asp Val Ala His Ile545 550 555 560ttt gtt ctt cct gaa ctt cca gcg tct cgc cgt gag cgt ctc gcg tgg 1728Phe Val Leu Pro Glu Leu Pro Ala Ser Arg Arg Glu Arg Leu Ala Trp
565 570 575cgc gaa atc aag acc tac cgc gcg taatttccca tctctgtacc ttc 1775Arg Glu Ile Lys Thr Tyr Arg Ala
580<210>360<211>584<212>PRT<213>谷氨酸棒杆菌<400>360Asp Ala Trp Ser Asp Pro Met Ala Thr Trp Arg His Ala Ile Thr Thr1 5 10 15Lys Ile Glu Ala Gly Gln Gly Ser Asp Glu Leu Tyr Asn Asp Phe Glu
20 25 30His Gly Ala Gln Leu Phe Glu Arg Ala Ala Glu Asn Leu Ser Lys Glu
35 40 45Asp Arg Thr Ala Leu Phe Asp Val Ala Ser Ser Leu Arg Arg Gly Gly
50 55 60Asp Val Arg Ala Arg Leu Ala Pro Ala Leu Thr Ala Ser Val Thr His65 70 75 80Leu Leu Glu Leu Asn Pro Leu Arg Glu Leu Val Thr Met Gly Glu Asn
85 90 95Leu Gln Val Arg Val Glu Arg Arg Ala Ala Leu Val Asn Ser Trp Tyr
100 105 110Glu Leu Phe Pro Arg Ser Thr Gly Gly Trp Asp Glu Ser Gly Thr Pro
115 120 125Val His Gly Thr Phe Ala Thr Thr Ala Gln Ala Leu Glu Arg Val Ala
130 135 140Lys Met Gly Phe Asp Thr Val Tyr Phe Pro Pro Ile His Pro Ile Gly145 150 155 160Glu Val Asn Arg Lys Gly Arg Asn Asn Thr Leu Thr Pro Glu Pro His
165 170 175Asp Val Gly Ser Pro Trp Ala Ile Gly Ser Lys Asp Gly Gly His Asp
180 185 190Ala Thr His Pro Arg Leu Gly Thr Ile Glu Asp Phe Gln Ala Leu Leu
195 200 205Ala Arg Ala Arg Glu Leu Asn Leu Glu Val Ala Leu Asp Leu Ala Leu
210 215 220Gln Ala Ala Pro Asp His Pro Trp Ala Gln Glu His Arg Glu Phe Phe225 230 235 240Thr Val Leu Ala Asp Gly Thr Ile Ala Tyr Ala Glu Asn Pro Pro Lys
245 250 255Lys Tyr Gln Asp Ile Tyr Pro Ile Asn Phe Asp Asn Asp Ala Pro Lys
260 265 270Ile Tyr Glu Glu Val Tyr Arg Val Val Lys Phe Trp Val Asp Leu Gly
275 280 285Val Thr Thr Phe Arg Val Asp Asn Pro His Thr Lys Pro Ala Asn Phe
290 295 300Trp Gln Trp Leu Ile Ser Ala Ile His Lys Ser Asn Pro Glu Val Ile305 310 315 320Phe Leu Ala Glu Ala Ser Thr Arg Pro Ala Arg Leu Tyr Phe Leu Ser
325 330 335Lys Ile Gly Phe Ser Gln Ser Tyr Thr Tyr Phe Thr Trp Lys Val Thr
340 345 350Asn Glu Glu Leu Thr Glu Phe Ala Thr Glu Ile Ala Pro Met Ala Asp
355 360 365Ile Ser Arg Pro Asn Leu Phe Val Asn Thr Pro Asp Ile Leu His Ala
370 375 380Ser Leu Gln His Gly Gly Arg Ala Met Phe Ala Ile Arg Ala Ala Leu385 390 395 400Ala Ala Thr Met Ser Pro Val Trp Gly Val Tyr Ser Gly Tyr Glu Leu
405 410 415Phe Glu His Glu Ala Val Lys Pro Gly Ser Glu Glu Tyr Leu Asp Ser
420 425 430Glu Lys Tyr Glu Leu Arg Pro Arg Asp Phe Glu Gly Ala Leu Glu Arg
435 440 445Gly Asp Ser Leu Glu Asp Tyr Ile Ala Leu Leu Asn Gln Ile Arg Arg
450 455 460Ala Asn Pro Ala Leu Gln Gln Leu Arg Asn Ile His Phe His Glu Ala465 470 475 480Asp Asn Asp Gln Ile Ile Ala Tyr Ser Lys Val Asp Ala Leu Thr Gly
485 490 495Asn Thr Val Leu Ile Val Val Asn Leu Asp Pro Arg Ser Ala Arg Glu
500 505 510Ala Thr Val Arg Leu Asp Leu Gly Ala Leu Gly Leu Glu Ala Gly Ala
515 520 525Gln Phe Glu Val Arg Asp Ala Ile Thr Gly Ser Arg Tyr Leu Trp Ser
530 535 540Glu Thr Asn Phe Val Arg Leu Glu Pro Leu Arg Asp Val Ala His Ile545 550 555 560Phe Val Leu Pro Glu Leu Pro Ala Ser Arg Arg Glu Arg Leu Ala Trp
565 570 575Arg Glu Ile Lys Thr Tyr Arg Ala
580<210>361<211>2316<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2293)<223>RXA02612<400>361gaacttccag cgtctcgccg tgagcgtctc gcgtggcgcg aaatcaagac ctaccgcgcg 60taatttccca tctctgtacc ttctatcaag gattatcatc atg acc gtt gac ccc 115
Met Thr Val Asp Pro
1 5gcg agc cac atc acc atc cct gaa gca gat ctg gcc cgc ctg cgc cac 163Ala Ser His Ile Thr Ile Pro Glu Ala Asp Leu Ala Arg Leu Arg His
10 15 20tgc aac cat cac gat cct cat gga ttt tat ggt tgg cat gag acc gaa 211Cys Asn His His Asp Pro His Gly Phe Tyr Gly Trp His Glu Thr Glu
25 30 35gct ggt tcg gtt atc cgc acg cgc cag gtc ggc gcg acg cag gtt aat 259Ala Gly Ser Val Ile Arg Thr Arg Gln Val Gly Ala Thr Gln Val Asn
40 45 50ttg ctt atc gac gac acc tcc cac gtc atg acc cct atc ggc gac gac 307Leu Leu Ile Asp Asp Thr Ser His Val Met Thr Pro Ile Gly Asp Asp
55 60 65att ttc gca att gac tta ggt cac cgc gag cgc gct gac tat cgc ttg 355Ile Phe Ala Ile Asp Leu Gly His Arg Glu Arg Ala Asp Tyr Arg Leu70 75 80 85gaa gtc acc tgg cct gat caa gaa ccg cag gtc aag gct gat cca tac 403Glu Val Thr Trp Pro Asp Gln Glu Pro Gln Val Lys Ala Asp Pro Tyr
90 95 100tac ttc ctc ccc acc gta ggc gag atg gat att tac ctc ttc tct gag 451Tyr Phe Leu Pro Thr Val Gly Glu Met Asp Ile Tyr Leu Phe Ser Glu
105 110 115gga cgc cat gag cgt ttg tgg gag att ctc ggt gcc aac atc aag acc 499Gly Arg His Glu Arg Leu Trp Glu Ile Leu Gly Ala Asn Ile Lys Thr
120 125 130tac caa act gcg ctc gga aca gtt cgt ggc acc gca ttt act gtg tgg 547Tyr Gln Thr Ala Leu Gly Thr Val Arg Gly Thr Ala Phe Thr Val Trp
135 140 145gct cca aac gca att ggc tgc gca gtg gtc ggt ggc ttc aac ggt tgg 595Ala Pro Asn Ala Ile Gly Cys Ala Val Val Gly Gly Phe Asn Gly Trp150 155 160 165aat gca tcc cag cat ccg atg cgt tct atg ggt ggt tcg ggt ctg tgg 643Asn Ala Ser Gln His Pro Met Arg Ser Met Gly Gly Ser Gly Leu Trp
170 175 180gag ctg ttc atc cca ggc ata gag gaa ggc gaa gtg tac aaa ttc gcc 691Glu Leu Phe Ile Pro Gly Ile Glu Glu Gly Glu Val Tyr Lys Phe Ala
185 190 195gtc caa acc agg gaa ggc caa cgt cgt gat aag gcc gat ccg atg gct 739Val Gln Thr Arg Glu Gly Gln Arg Arg Asp Lys Ala Asp Pro Met Ala
200 205 210cgt cgc gca gaa ctg gcg ccg gca acc gga tct att gtc gct tcc tct 787Arg Arg Ala Glu Leu Ala Pro Ala Thr Gly Ser Ile Val Ala Ser Ser
215 220 225gag tac cag tgg cag gat tcc gag tgg ctg cgc gag cgt tcc caa act 835Glu Tyr Gln Trp Gln Asp Ser Glu Trp Leu Arg Glu Arg Ser Gln Thr230 235 240 245gat ctc gca tcc aag cca atg agt gtc tac gag gtc cac ctc ggt tct 883Asp Leu Ala Ser Lys Pro Met Ser Val Tyr Glu Val His Leu Gly Ser
250 255 260tgg cgc tgg ggt aag aac tat gag gat ttg gct act gag ctg gtt gat 931Trp Arg Trp Gly Lys Asn Tyr Glu Asp Leu Ala Thr Glu Leu Val Asp
265 270 275tac gtc gca gat ctt ggc tac acc cac gtg gaa ttc ctc cct gtc gca 979Tyr Val Ala Asp Leu Gly Tyr Thr His Val Glu Phe Leu Pro Val Ala
280 285 290gag cac ccc ttc ggt ggt tcc tgg ggt tac cag gtc acc ggc tac tac 1027Glu His Pro Phe Gly Gly Ser Trp Gly Tyr Gln Val Thr Gly Tyr Tyr
295 300 305gca ccg acc tct cgt tgg ggt act cca gat cag ttc cgt gcg cta gtc 1075Ala Pro Thr Ser Arg Trp Gly Thr Pro Asp Gln Phe Arg Ala Leu Val310 315 320 325gac gct ttc cac gcc cgc ggt att ggc gtg atc atg gac tgg gtt cct 1123Asp Ala Phe His Ala Arg Gly Ile Gly Val Ile Met Asp Trp Val Pro
330 335 340gcc cac ttc cct aag gat gat tgg gct ctt gcc cgc ttt gat ggc gaa 1171Ala His Phe Pro Lys Asp Asp Trp Ala Leu Ala Arg Phe Asp Gly Glu
345 350 355gcc ctc tat gaa cac cct gac tgg agg cgc ggc gaa caa aag gat tgg 1219Ala Leu Tyr Glu His Pro Asp Trp Arg Arg Gly Glu Gln Lys Asp Trp
360 365 370ggc acc ctg gtc ttt gac ttc ggt cgc aac gaa gtc cgc aac ttc ctc 1267Gly Thr Leu Val Phe Asp Phe Gly Arg Asn Glu Val Arg Asn Phe Leu
375 380 385gtc gct aat gcg ttg tac tgg att gaa gaa ttc cac atc gat ggt ctg 1315Val Ala Asn Ala Leu Tyr Trp Ile Glu Glu Phe His Ile Asp Gly Leu390 395 400 405cgc gtc gac gcc gtg gcc tcc atg ctg tac ctc gat tac tcc cgt gag 1363Arg Val Asp Ala Val Ala Ser Met Leu Tyr Leu Asp Tyr Ser Arg Glu
410 415 420cac ggc gaa tgg gaa cca aac atc tac ggt ggt cgc gag aac ctc gag 1411His Gly Glu Trp Glu Pro Asn Ile Tyr Gly Gly Arg Glu Asn Leu Glu
425 430 435gca gtg cag ttc ctg cag gaa atg aac gcc acg gtg ctg cga ctg cac 1459Ala Val Gln Phe Leu Gln Glu Met Asn Ala Thr Val Leu Arg Leu His
440 445 450cct ggt gcg ctc acc atc gct gag gaa tca act tca tgg cct ggc gtg 1507Pro Gly Ala Leu Thr Ile Ala Glu Glu Ser Thr Ser Trp Pro Gly Val
455 460 465acc gca cca acg tgg gac ggc ggc ttg gga ttc tcc ctc aag tgg aac 1555Thr Ala Pro Thr Trp Asp Gly Gly Leu Gly Phe Ser Leu Lys Trp Asn470 475 480 485atg ggc tgg atg cac gac acc tta gag tac ttc tcc aaa aac cct gtg 1603Met Gly Trp Met His Asp Thr Leu Glu Tyr Phe Ser Lys Asn Pro Val
490 495 500cac cgc gca ttc cac cac agt gag ctc act ttc tcc ttg gtg tac gca 1651His Arg Ala Phe His His Ser Glu Leu Thr Phe Ser Leu Val Tyr Ala
505 510 515ttc tct gag cgt ttt gta ctt ccg atc tct cac gat gaa gtc gtc cac 1699Phe Ser Glu Arg Phe Val Leu Pro Ile Ser His Asp Glu Val Val His
520 525 530ggc aag ggt tcc ctg tgg gac cgt atg cct ggc gat acg tgg aac aag 1747Gly Lys Gly Ser Leu Trp Asp Arg Met Pro Gly Asp Thr Trp Asn Lys
535 540 545gcc gct ggt ctt cgc acc ttc ctt gcg tac atg tgg tca cac cca ggc 1795Ala Ala Gly Leu Arg Thr Phe Leu Ala Tyr Met Trp Ser His Pro Gly550 555 560 565aag aag ctg ctt ttc atg ggt cag gag ttt ggt cag cgt gaa gag tgg 1843Lys Lys Leu Leu Phe Met Gly Gln Glu Phe Gly Gln Arg Glu Glu Trp
570 575 580gct gaa ggc cag gga ctg cca tgg gat att gtc gac ggc tgg caa ggc 1891Ala Glu Gly Gln Gly Leu Pro Trp Asp Ile Val Asp Gly Trp Gln Gly
585 590 595gag tac cac gaa gcc atc cgc act ctg acc cgc tcc ctc aac ggt gtc 1939Glu Tyr His Glu Ala Ile Arg Thr Leu Thr Arg Ser Leu Asn Gly Val
600 605 610tac tca gac tcc cct gcg ctg cac act cag gat ttc acc gga gaa ggc 1987Tyr Ser Asp Ser Pro Ala Leu His Thr Gln Asp Phe Thr Gly Glu Gly
615 620 625ttc aca tgg aat aag ggc gac gac gcc acc aac aac att ttg gcg ttc 2035Phe Thr Trp Asn Lys Gly Asp Asp Ala Thr Asn Asn Ile Leu Ala Phe630 635 640 645act cgt ttc ggc agc gac ggc tcc cag atg ctg tgt gta ttc aac ctg 2083Thr Arg Phe Gly Ser Asp Gly Ser Gln Met Leu Cys Val Phe Asn Leu
650 655 660tct gga acc tcc cag cct gag tac cag ctc ggt gtt gca gcg ggt ggc 2131Ser Gly Thr Ser Gln Pro Glu Tyr Gln Leu Gly Val Ala Ala Gly Gly
665 670 675gaa tgg aag ctc gtt ctc aac act gat gat gct gaa ttc ctc ggt gca 2179Glu Trp Lys Leu Val Leu Asn Thr Asp Asp Ala Glu Phe Leu Gly Ala
680 685 690gaa aac gat atc gct act tcc gtt caa gca gca gcg aca cca cgc gat 2227Glu Asn Asp Ile Ala Thr Ser Val Gln Ala Ala Ala Thr Pro Arg Asp
695 700 705aat ttc gcc tac tca ctc tca ctg cat gtc cca gcg atg agt gct cag 2275Asn Phe Ala Tyr Ser Leu Ser Leu His Val Pro Ala Met Ser Ala Gln710 715 720 725ttc tac tca ctg cag aag taggacacag gaaaatgcat cct 2316Phe Tyr Ser Leu Gln Lys
730<210>362<211>731<212>PRT<213>谷氨酸棒杆菌<400>362Met Thr Val Asp Pro Ala Ser His Ile Thr Ile Pro Glu Ala Asp Leu1 5 10 15Ala Arg Leu Arg His Cys Asn His His Asp Pro His Gly Phe Tyr Gly
20 25 30Trp His Glu Thr Glu Ala Gly Ser Val Ile Arg Thr Arg Gln Val Gly
35 40 45Ala Thr Gln Val Asn Leu Leu Ile Asp Asp Thr Ser His Val Met Thr
50 55 60Pro Ile Gly Asp Asp Ile Phe Ala Ile Asp Leu Gly His Arg Glu Arg65 70 75 80Ala Asp Tyr Arg Leu Glu Val Thr Trp Pro Asp Gln Glu Pro Gln Val
85 90 95Lys Ala Asp Pro Tyr Tyr Phe Leu Pro Thr Val Gly Glu Met Asp Ile
100 105 110Tyr Leu Phe Ser Glu Gly Arg His Glu Arg Leu Trp Glu Ile Leu Gly
115 120 125Ala Asn Ile Lys Thr Tyr Gln Thr Ala Leu Gly Thr Val Arg Gly Thr
130 135 140Ala Phe Thr Val Trp Ala Pro Asn Ala Ile Gly Cys Ala Val Val Gly145 150 155 160Gly Phe Asn Gly Trp Asn Ala Ser Gln His Pro Met Arg Ser Met Gly
165 170 175Gly Ser Gly Leu Trp Glu Leu Phe Ile Pro Gly Ile Glu Glu Gly Glu
180 185 190Val Tyr Lys Phe Ala Val Gln Thr Arg Glu Gly Gln Arg Arg Asp Lys
195 200 205Ala Asp Pro Met Ala Arg Arg Ala Glu Leu Ala Pro Ala Thr Gly Ser
210 215 220Ile Val Ala Ser Ser Glu Tyr Gln Trp Gln Asp Ser Glu Trp Leu Arg225 230 235 240Glu Arg Ser Gln Thr Asp Leu Ala Ser Lys Pro Met Ser Val Tyr Glu
245 250 255Val His Leu Gly Ser Trp Arg Trp Gly Lys Asn Tyr Glu Asp Leu Ala
260 265 270Thr Glu Leu Val Asp Tyr Val Ala Asp Leu Gly Tyr Thr His Val Glu
275 280 285Phe Leu Pro Val Ala Glu His Pro Phe Gly Gly Ser Trp Gly Tyr Gln
290 295 300Val Thr Gly Tyr Tyr Ala Pro Thr Ser Arg Trp Gly Thr Pro Asp Gln305 310 315 320Phe Arg Ala Leu Val Asp Ala Phe His Ala Arg Gly Ile Gly Val Ile
325 330 335Met Asp Trp Val Pro Ala His Phe Pro Lys Asp Asp Trp Ala Leu Ala
340 345 350Arg Phe Asp Gly Glu Ala Leu Tyr Glu His Pro Asp Trp Arg Arg Gly
355 360 365Glu Gln Lys Asp Trp Gly Thr Leu Val Phe Asp Phe Gly Arg Asn Glu
370 375 380Val Arg Asn Phe Leu Val Ala Asn Ala Leu Tyr Trp Ile Glu Glu Phe385 390 395 400His Ile Asp Gly Leu Arg Val Asp Ala Val Ala Ser Met Leu Tyr Leu
405 410 415Asp Tyr Ser Arg Glu His Gly Glu Trp Glu Pro Asn Ile Tyr Gly Gly
420 425 430Arg Glu Asn Leu Glu Ala Val Gln Phe Leu Gln Glu Met Asn Ala Thr
435 440 445Val Leu Arg Leu His Pro Gly Ala Leu Thr Ile Ala Glu Glu Ser Thr
450 455 460Ser Trp Pro Gly Val Thr Ala Pro Thr Trp Asp Gly Gly Leu Gly Phe465 470 475 480Ser Leu Lys Trp Asn Met Gly Trp Met His Asp Thr Leu Glu Tyr Phe
485 490 495Ser Lys Asn Pro Val His Arg Ala Phe His His Ser Glu Leu Thr Phe
500 505 510Ser Leu Val Tyr Ala Phe Ser Glu Arg Phe Val Leu Pro Ile Ser His
515 520 525Asp Glu Val Val His Gly Lys Gly Ser Leu Trp Asp Arg Met Pro Gly
530 535 540Asp Thr Trp Asn Lys Ala Ala Gly Leu Arg Thr Phe Leu Ala Tyr Met545 550 555 560Trp Ser His Pro Gly Lys Lys Leu Leu Phe Met Gly Gln Glu Phe Gly
565 570 575Gln Arg Glu Glu Trp Ala Glu Gly Gln Gly Leu Pro Trp Asp Ile Val
580 585 590Asp Gly Trp Gln Gly Glu Tyr His Glu Ala Ile Arg Thr Leu Thr Arg
595 600 605Ser Leu Asn Gly Val Tyr Ser Asp Ser Pro Ala Leu His Thr Gln Asp
610 615 620Phe Thr Gly Glu Gly Phe Thr Trp Asn Lys Gly Asp Asp Ala Thr Asn625 630 635 640Asn Ile Leu Ala Phe Thr Arg Phe Gly Ser Asp Gly Ser Gln Met Leu
645 650 655Cys Val Phe Asn Leu Ser Gly Thr Ser Gln Pro Glu Tyr Gln Leu Gly
660 665 670Val Ala Ala Gly Gly Glu Trp Lys Leu Val Leu Asn Thr Asp Asp Ala
675 680 685Glu Phe Leu Gly Ala Glu Asn Asp Ile Ala Thr Ser Val Gln Ala Ala
690 695 700Ala Thr Pro Arg Asp Asn Phe Ala Tyr Ser Leu Ser Leu His Val Pro705 710 715 720Ala Met Ser Ala Gln Phe Tyr Ser Leu Gln Lys
725 730<210>363<211>1913<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1890)<223>RXN01884<400>363gtg act gcc att gaa ttg atg ccg gtc cac cag ttc ctg cag gat gat 48Val Thr Ala Ile Glu Leu Met Pro Val His Gln Phe Leu Gln Asp Asp1 5 10 15cgt ctc cgc gac cta gga atg cgc aac tac tgg ggc tac aac tct ttc 96Arg Leu Arg Asp Leu Gly Met Arg Asn Tyr Trp Gly Tyr Asn Ser Phe
20 25 30ggc ttc ttt gcg ccc tac aac gat tac gct gcc aac aag aac ccc ggt 144Gly Phe Phe Ala Pro Tyr Asn Asp Tyr Ala Ala Asn Lys Asn Pro Gly
35 40 45ggc gcc gtg gcc gag ttc aaa ggc ttg gtg cgc agc Lac cac gaa gcg 192Gly Ala Val Ala Glu Phe Lys Gly Leu Val Arg Ser Tyr His Glu Ala
50 55 60ggc ctc gaa gta att ttg gat gtg gtc tac aac cac acc gcc gag ggc 240Gly Leu Glu Val Ile Leu Asp Val Val Tyr Asn His Thr Ala Glu Gly65 70 75 80aac cac atg ggc ccc acc atc gcg ttc cga ggc att gac aac gag gcg 288Asn His Met Gly Pro Thr Ile Ala Phe Arg Gly Ile Asp Asn Glu Ala
85 90 95tac tac cga ctg gtt gag ggc gat cgc cgt cat tac atg gac tac acc 336Tyr Tyr Arg Leu Val Glu Gly Asp Arg Arg His Tyr Met Asp Tyr Thr
100 105 110ggt act ggt aac tcc ctc aat gtc cgc gac ccg cac tca ctg cag ctg 384Gly Thr Gly Asn Ser Leu Asn Val Arg Asp Pro His Ser Leu Gln Leu
115 120 125att atg gat tcg ctg cgc tac tgg gtc acc gag atg cat gtc gac ggc 432Ile Met Asp Ser Leu Arg Tyr Trp Val Thr Glu Met His Val Asp Gly
130 135 140ttc cgc ttc gac ctt gcc tct acc ctt gct cgt gaa ttt gat gat gtt 480Phe Arg Phe Asp Leu Ala Ser Thr Leu Ala Arg Glu Phe Asp Asp Val145 150 155 160gac cgc ctg gca acc ttc ttc gac ctg gtc caa caa gac ccg gtg gtc 528Asp Arg Leu Ala Thr Phe Phe Asp Leu Val Gln Gln Asp Pro Val Val
165 170 175tcc cag gtc aag ctc att gct gag ccg tgg gat gtt ggc gaa ggc gga 576Ser Gln Val Lys Leu Ile Ala Glu Pro Trp Asp Val Gly Glu Gly Gly
180 185 190tac caa gtg ggt aac ttc cca cca ctg tgg act gag tgg aac ggt aaa 624Tyr Gln Val Gly Asn Phe Pro Pro Leu Trp Thr Glu Trp Asn Gly Lys
195 200 205tac cgc gac act gtc cgt gat ttc tgg cgt ggt gag cca gca acc ttg 672Tyr Arg Asp Thr Val Arg Asp Phe Trp Arg Gly Glu Pro Ala Thr Leu
210 215 220ggt gaa ttc gct tcc cga cra act ggt tcc tct gat ttg tat gca aac 720Gly Glu Phe Ala Ser Arg Leu Thr Gly Ser Ser Asp Leu Tyr Ala Asn225 230 235 240aac ggc cgt cgc ccc act gca tcg arc aac ttt gtg act gct cac gac 768Asn Gly Arg Arg Pro Thr Ala Ser Ile Asn Phe Val Thr Ala His Asp
245 250 255ggc ttc acc ctc aat gac ttg gtc agt tac aac gag aag cac aac atg 816Gly Phe Thr Leu Asn Asp Leu Val Ser Tyr Asn Glu Lys His Asn Met
260 265 270gcc aac ggt gaa gac ggt cgg gac ggt gaa tca cac aac cgt tcc tgg 864Ala Asn Gly Glu Asp Gly Arg Asp Gly Glu Ser His Asn Arg Ser Trp
275 280 285aac tgt ggc gtc gaa gga cca act gac gat cct gag att atg cag ctg 912Asn Cys Gly Val Glu Gly Pro Thr Asp Asp Pro Glu Ile Met Gln Leu
290 295 300cgt gct cag caa cga cgc aac ttc ctc acc acc ttg ttg ctg tcc cag 960Arg Ala Gln Gln Arg Arg Asn Phe Leu Thr Thr Leu Leu Leu Ser Gln305 310 315 320ggc acc cct atg ttg tcc cac ggt gat gaa atg gcc cgt acc caa aac 1008Gly Thr Pro Met Leu Ser His Gly Asp Glu Met Ala Arg Thr Gln Asn
325 330 335ggc aac aac aac gtc tac tgc caa gac aat gaa ctg gcg tgg gtg aat 1056Gly Asn Asn Asn Val Tyr Cys Gln Asp Asn Glu Leu Ala Trp Val Asn
340 345 350tgg gat cag gct gaa gaa aac gct gac ttg gtg agc ttc acc agg cgt 1104Trp Asp Gln Ala Glu Glu Asn Ala Asp Leu Val Ser Phe Thr Arg Arg
355 360 365ttg ctg cgt atc cga gca aac cac cca gta ttt agg cgc agg cag ttc 1152Leu Leu Arg Ile Arg Ala Asn His Pro Val Phe Arg Arg Arg Gln Phe
370 375 380ctt gcc ggt ggc cct ttg ggc gcc gat gtt cgt gac cgc gat atc gca 1200Leu Ala Gly Gly Pro Leu Gly Ala Asp Val Arg Asp Arg Asp Ile Ala385 390 395 400tgg ctg gta cca aat gga acc ttg atg act caa gat gac tgg gac ttc 1248Trp Leu Val Pro Asn Gly Thr Leu Met Thr Gln Asp Asp Trp Asp Phe
405 410 415gct ttc ggt aaa tca ctg cag gtg ttc ttc aac ggc gat gcc atc gaa 1296Ala Phe Gly Lys Ser Leu Gln Val Phe Phe Asn Gly Asp Ala Ile Glu
420 425 430gag cct gat tat cga gga cag aaa atc cac gat gac tcc ttc atc ttg 1344Glu Pro Asp Tyr Arg Gly Gln Lys Ile His Asp Asp Ser Phe Ile Leu
435 440 445atg ttc aac gct cac ttc gaa cct atc gat ttc aat ctc cct cct gag 1392Met Phe Asn Ala His Phe Glu Pro Ile Asp Phe Asn Leu Pro Pro Glu
450 455 460cat ttc ggt atg aag tgg aag ctt ttg gtc gat acc acc gaa gcg gtg 1440His Phe Gly Met Lys Trp Lys Leu Leu Val Asp Thr Thr Glu Ala Val465 470 475 480ggc cac ccg ctg gag gat ctc acc atc gaa gct ggc gga acc atc act 1488Gly His Pro Leu Glu Asp Leu Thr Ile Glu Ala Gly Gly Thr Ile Thr
485 490 495gtt cct gcc cgt tcc acg atg ctg ctg cgc cag gtg gag gct ccg gac 1536Val Pro Ala Arg Ser Thr Met Leu Leu Arg Gln Val Glu Ala Pro Asp
500 505 510tac acc aag ctt gag gaa aag atc gct gct gaa aag cgt gag caa gaa 1584Tyr Thr Lys Leu Glu Glu Lys Ile Ala Ala Glu Lys Arg Glu Gln Glu
515 520 525ctt gcg gca gag aag gaa gct gct gag aag cgc gaa ttg gaa ctg gcg 1632Leu Ala Ala Glu Lys Glu Ala Ala Glu Lys Arg Glu Leu Glu Leu Ala
530 535 540gca gca aag gaa gct gaa gat gct gct gag gct ctc cac ctt gcg gca 1680Ala Ala Lys Glu Ala Glu Asp Ala Ala Glu Ala Leu His Leu Ala Ala545 550 555 560gaa cgt gct tcg act cag gaa gct gaa ttg gcc cat caa cac ggt gct 1728Glu Arg Ala Ser Thr Gln Glu Ala Glu Leu Ala His Gln His Gly Ala
565 570 575gat gcg att gcc gat gag gta gcg gaa gaa cca caa gag ctg cca caa 1776Asp Ala Ile Ala Asp Glu Val Ala Glu Glu Pro Gln Glu Leu Pro Gln
580 585 590gat gaa gta gcg gca gag gtc gag act gag ccc gac acc gag cct gac 1824Asp Glu Val Ala Ala Glu Val Glu Thr Glu Pro Asp Thr Glu Pro Asp
595 600 605act gaa tct gac tcc gag cag gct gag gta gct tca gag gag cct gaa 1872Thr Glu Ser Asp Ser Glu Gln Ala Glu Val Ala Ser Glu Glu Pro Glu
610 615 620gcg gac gaa gaa gag aag tagtacaccg aaagtggcgt cgc 1913Ala Asp Glu Glu Glu Lys625 630<210>364<211>630<212>PRT<213>谷氨酸棒杆菌<400>364Val Thr Ala Ile Glu Leu Met Pro Val His Gln Phe Leu Gln Asp Asp1 5 10 15Arg Leu Arg Asp Leu Gly Met Arg Asn Tyr Trp Gly Tyr Asn Ser Phe
20 25 30Gly Phe Phe Ala Pro Tyr Asn Asp Tyr Ala Ala Asn Lys Asn Pro Gly
35 40 45Gly Ala Val Ala Glu Phe Lys Gly Leu Val Arg Ser Tyr His Glu Ala
50 55 60Gly Leu Glu Val Ile Leu Asp Val Val Tyr Asn His Thr Ala Glu Gly65 70 75 80Asn His Met Gly Pro Thr Ile Ala Phe Arg Gly Ile Asp Asn Glu Ala
85 90 95Tyr Tyr Arg Leu Val Glu Gly Asp Arg Arg His Tyr Met Asp Tyr Thr
100 105 110Gly Thr Gly Asn Ser Leu Asn Val Arg Asp Pro His Ser Leu Gln Leu
115 120 125Ile Met Asp Ser Leu Arg Tyr Trp Val Thr Glu Met His Val Asp Gly
130 135 140Phe Arg Phe Asp Leu Ala Ser Thr Leu Ala Arg Glu Phe Asp Asp Val145 150 155 160Asp Arg Leu Ala Thr Phe Phe Asp Leu Val Gln Gln Asp Pro Val Val
165 170 175Ser Gln Val Lys Leu Ile Ala Glu Pro Trp Asp Val Gly Glu Gly Gly
180 185 190Tyr Gln Val Gly Asn Phe Pro Pro Leu Trp Thr Glu Trp Asn Gly Lys
195 200 205Tyr Arg Asp Thr Val Arg Asp Phe Trp Arg Gly Glu Pro Ala Thr Leu
210 215 220Gly Glu Phe Ala Ser Arg Leu Thr Gly Ser Ser Asp Leu Tyr Ala Asn225 230 235 240Asn Gly Arg Arg Pro Thr Ala Ser Ile Asn Phe Val Thr Ala His Asp
245 250 255Gly Phe Thr Leu Asn Asp Leu Val Ser Tyr Asn Glu Lys His Asn Met
260 265 270Ala Asn Gly Glu Asp Gly Arg Asp Gly Glu Ser His Asn Arg Ser Trp
275 280 285Asn Cys Gly Val Glu Gly Pro Thr Asp Asp Pro Glu Ile Met Gln Leu
290 295 300Arg Ala Gln Gln Arg Arg Asn Phe Leu Thr Thr Leu Leu Leu Ser Gln305 310 315 320Gly Thr Pro Met Leu Ser His Gly Asp Glu Met Ala Arg Thr Gln Asn
325 330 335Gly Asn Asn Asn Val Tyr Cys Gln Asp Asn Glu Leu Ala Trp Val Asn
340 345 350Trp Asp Gln Ala Glu Glu Asn Ala Asp Leu Val Ser Phe Thr Arg Arg
355 360 365Leu Leu Arg Ile Arg Ala Asn His Pro Val Phe Arg Arg Arg Gln Phe
370 375 380Leu Ala Gly Gly Pro Leu Gly Ala Asp Val Arg Asp Arg Asp Ile Ala385 390 395 400Trp Leu Val Pro Asn Gly Thr Leu Met Thr Gln Asp Asp Trp Asp Phe
405 410 415Ala Phe Gly Lys Ser Leu Gln Val Phe Phe Asn Gly Asp Ala Ile Glu
420 425 430Glu Pro Asp Tyr Arg Gly Gln Lys Ile His Asp Asp Ser Phe Ile Leu
435 440 445Met Phe Asn Ala His Phe Glu Pro Ile Asp Phe Asn Leu Pro Pro Glu
450 455 460His Phe Gly Met Lys Trp Lys Leu Leu Val Asp Thr Thr Glu Ala Val465 470 475 480Gly His Pro Leu Glu Asp Leu Thr Ile Glu Ala Gly Gly Thr Ile Thr
485 490 495Val Pro Ala Arg Ser Thr Met Leu Leu Arg Gln Val Glu Ala Pro Asp
500 505 510Tyr Thr Lys Leu Glu Glu Lys Ile Ala Ala Glu Lys Arg Glu Gln Glu
515 520 525Leu Ala Ala Glu Lys Glu Ala Ala Glu Lys Arg Glu Leu Glu Leu Ala
530 535 540Ala Ala Lys Glu Ala Glu Asp Ala Ala Glu Ala Leu His Leu Ala Ala545 550 555 560Glu Arg Ala Ser Thr Gln Glu Ala Glu Leu Ala His Gln His Gly Ala
565 570 575Asp Ala Ile Ala Asp Glu Val Ala Glu Glu Pro Gln Glu Leu Pro Gln
580 585 590Asp Glu Val Ala Ala Glu Val Glu Thr Glu Pro Asp Thr Glu Pro Asp
595 600 605Thr Glu Ser Asp Ser Glu Gln Ala Glu Val Ala Ser Glu Glu Pro Glu
610 615 620Ala Asp Glu Glu Glu Lys625 630<210>365<211>1496<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1473)<223>FRXA01884<400>365atg cat gtc gac ggc ttc cgc ttc gac ctt gcc tct acc ctt gct cgt 48Met His Val Asp Gly Phe Arg Phe Asp Leu Ala Ser Thr Leu Ala Arg1 5 10 15gaa ttt gat gat gtt gac cgc ctg gca acc ttc ttc gac ctg gtc caa 96Glu Phe Asp Asp Val Asp Arg Leu Ala Thr Phe Phe Asp Leu Val Gln
20 25 30caa gac ccg gtg gtc tcc cag gtc aag ctc att gct gag ccg tgg gat 144Gln Asp Pro Val Val Ser Gln Val Lys Leu Ile Ala Glu Pro Trp Asp
35 40 45gtt ggc gaa ggc gga tac caa gtg ggt aac ttc cca cca ctg tgg act 192Val Gly Glu Gly Gly Tyr Gln Val Gly Asn Phe Pro Pro Leu Trp Thr
50 55 60gag tgg aac ggt aaa tac cgc gac act gtc cgt gat ttc tgg cgt ggt 240Glu Trp Asn Gly Lys Tyr Arg Asp Thr Val Arg Asp Phe Trp Arg Gly65 70 75 80gag cca gca acc ttg ggt gaa ttc gct tcc cga cta act ggt tcc tct 288Glu Pro Ala Thr Leu Gly Glu Phe Ala Ser Arg Leu Thr Gly Ser Ser
85 90 95gat ttg tat gca aac aac ggc cgt cgc ccc act gca tcg atc aac ttt 336Asp Leu Tyr Ala Asn Asn Gly Arg Arg Pro Thr Ala Ser Ile Asn Phe
100 105 110gtg act gct cac gac ggc ttc acc ctc aat gac ttg gtc agt tac aac 384Val Thr Ala His Asp Gly Phe Thr Leu Asn Asp Leu Val Ser Tyr Asn
115 120 125gag aag cac aac atg gcc aac ggt gaa gac ggt cgg gac ggt gaa tca 432Glu Lys His Asn Met Ala Asn Gly Glu Asp Gly Arg Asp Gly Glu Ser
130 135 140cac aac cgt tcc tgg aac tgt ggc gtc gaa gga cca act gac gat cct 480His Asn Arg Ser Trp Asn Cys Gly Val Glu Gly Pro Thr Asp Asp Pro145 150 155 160gag att atg cag ctg cgt gct cag caa cga cgc aac ttc ctc acc acc 528Glu Ile Met Gln Leu Arg Ala Gln Gln Arg Arg Asn Phe Leu Thr Thr
165 170 175ttg ttg ctg tcc cag ggc acc cct atg ttg tcc cac ggt gat gaa atg 576Leu Leu Leu Ser Gln Gly Thr Pro Met Leu Ser His Gly Asp Glu Met
180 185 190gcc cgt acc caa aac ggc aac aac aac gtc tac tgc caa gac aat gaa 624Ala Arg Thr Gln Asn Gly Asn Asn Asn Val Tyr Cys Gln Asp Asn Glu
195 200 205ctg gcg tgg gtg aat tgg gat cag gct gaa gaa aac gct gac ttg gtg 672Leu Ala Trp Val Asn Trp Asp Gln Ala Glu Glu Asn Ala Asp Leu Val
210 215 220agc ttc acc agg cgt ttg ctg cgt atc cga gca aac cac cca gta ttt 720Ser Phe Thr Arg Arg Leu Leu Arg Ile Arg Ala Asn His Pro Val Phe225 230 235 240agg cgc agg cag ttc ctt gcc ggt ggc cct ttg ggc gcc gat gtt cgt 768Arg Arg Arg Gln Phe Leu Ala Gly Gly Pro Leu Gly Ala Asp Val Arg
245 250 255gac cgc gat atc gca tgg ctg gta cca aat gga acc ttg atg act caa 816Asp Arg Asp Ile Ala Trp Leu Val Pro Asn Gly Thr Leu Met Thr Gln
260 265 270gat gac tgg gac ttc gct ttc ggt aaa tca ctg cag gtg ttc ttc aac 864Asp Asp Trp Asp Phe Ala Phe Gly Lys Ser Leu Gln Val Phe Phe Asn
275 280 285ggc gat gcc atc gaa gag cct gat tat cga gga cag aaa atc cac gat 912Gly Asp Ala Ile Glu Glu Pro Asp Tyr Arg Gly Gln Lys Ile His Asp
290 295 300gac tcc ttc atc ttg atg ttc aac gct cac ttc gaa cct atc gat ttc 960Asp Ser Phe Ile Leu Met Phe Asn Ala His Phe Glu Pro Ile Asp Phe305 310 315 320aat ctc cct cct gag cat ttc ggt atg aag tgg aag ctt ttg gtc gat 1008Asn Leu Pro Pro Glu His Phe Gly Met Lys Trp Lys Leu Leu Val Asp
325 330 335acc acc gaa gcg gtg ggc cac ccg ctg gag gat ctc acc atc gaa gct 1056Thr Thr Glu Ala Val Gly His Pro Leu Glu Asp Leu Thr Ile Glu Ala
340 345 350ggc gga acc atc act gtt cct gcc cgt tcc acg atg ctg ctg cgc cag 1104Gly Gly Thr Ile Thr Val Pro Ala Arg Ser Thr Met Leu Leu Arg Gln
355 360 365gtg gag gct ccg gac tac acc aag ctt gag gaa aag atc gct gct gaa 1152Val Glu Ala Pro Asp Tyr Thr Lys Leu Glu Glu Lys Ile Ala Ala Glu
370 375 380aag cgt gag caa gaa ctt gcg gca gag aag gaa gct gct gag aag cgc 1200Lys Arg Glu Gln Glu Leu Ala Ala Glu Lys Glu Ala Ala Glu Lys Arg385 390 395 400gaa ttg gaa ctg gcg gca gca aag gaa gct gaa gat gct gct gag gct 1248Glu Leu Glu Leu Ala Ala Ala Lys Glu Ala Glu Asp Ala Ala Glu Ala
405 410 415ctc cac ctt gcg gca gaa cgt gct tcg act cag gaa gct gaa ttg gcc 1296Leu His Leu Ala Ala Glu Arg Ala Ser Thr Gln Glu Ala Glu Leu Ala
420 425 430cat caa cac ggt gct gat gcg att gcc gat gag gta gcg gaa gaa cca 1344His Gln His Gly Ala Asp Ala Ile Ala Asp Glu Val Ala Glu Glu Pro
435 440 445caa gag ctg cca caa gat gaa gta gcg gca gag gtc gag act gag ccc 1392Gln Glu Leu Pro Gln Asp Glu Val Ala Ala Glu Val Glu Thr Glu Pro
450 455 460gac acc gag cct gac act gaa tct gac tcc gag cag gct gag gta gct 1440Asp Thr Glu Pro Asp Thr Glu Ser Asp Ser Glu Gln Ala Glu Val Ala465 470 475 480tca gag gag cct gaa gcg gac gaa gaa gag aag tagtacaccg aaagtggcgt 1493Ser Glu Glu Pro Glu Ala Asp Glu Glu Glu Lys
485 490cgc 1496<210>366<211>491<212>PRT<213>谷氨酸棒杆菌<400>366Met His Val Asp Gly Phe Arg Phe Asp Leu Ala Ser Thr Leu Ala Arg1 5 10 15Glu Phe Asp Asp Val Asp Arg Leu Ala Thr Phe Phe Asp Leu Val Gln
20 25 30Gln Asp Pro Val Val Ser Gln Val Lys Leu Ile Ala Glu Pro Trp Asp
35 40 45Val Gly Glu Gly Gly Tyr Gln Val Gly Asn Phe Pro Pro Leu Trp Thr
50 55 60Glu Trp Asn Gly Lys Tyr Arg Asp Thr Val Arg Asp Phe Trp Arg Gly65 70 75 80Glu Pro Ala Thr Leu Gly Glu Phe Ala Ser Arg Leu Thr Gly Ser Ser
85 90 95Asp Leu Tyr Ala Asn Asn Gly Arg Arg Pro Thr Ala Ser Ile Asn Phe
100 105 110Val Thr Ala His Asp Gly Phe Thr Leu Asn Asp Leu Val Ser Tyr Asn
115 120 125Glu Lys His Asn Met Ala Asn Gly Glu Asp Gly Arg Asp Gly Glu Ser
130 135 140His Asn Arg Ser Trp Asn Cys Gly Val Glu Gly Pro Thr Asp Asp Pro145 150 155 160Glu Ile Met Gln Leu Arg Ala Gln Gln Arg Arg Asn Phe Leu Thr Thr
165 170 175Leu Leu Leu Ser Gln Gly Thr Pro Met Leu Ser His Gly Asp Glu Met
180 185 190Ala Arg Thr Gln Asn Gly Asn Asn Asn Val Tyr Cys Gln Asp Asn Glu
195 200 205Leu Ala Trp Val Asn Trp Asp Gln Ala Glu Glu Asn Ala Asp Leu Val
210 215 220Ser Phe Thr Arg Arg Leu Leu Arg Ile Arg Ala Asn His Pro Val Phe225 230 235 240Arg Arg Arg Gln Phe Leu Ala Gly Gly Pro Leu Gly Ala Asp Val Arg
245 250 255Asp Arg Asp Ile Ala Trp Leu Val Pro Asn Gly Thr Leu Met Thr Gln
260 265 270Asp Asp Trp Asp Phe Ala Phe Gly Lys Ser Leu Gln Val Phe Phe Asn
275 280 285Gly Asp Ala Ile Glu Glu Pro Asp Tyr Arg Gly Gln Lys Ile His Asp
290 295 300Asp Ser Phe Ile Leu Met Phe Asn Ala His Phe Glu Pro Ile Asp Phe305 310 315 320Asn Leu Pro Pro Glu His Phe Gly Met Lys Trp Lys Leu Leu Val Asp
325 330 335Thr Thr Glu Ala Val Gly His Pro Leu Glu Asp Leu Thr Ile Glu Ala
340 345 350Gly Gly Thr Ile Thr Val Pro Ala Arg Ser Thr Met Leu Leu Arg Gln
355 360 365Val Glu Ala Pro Asp Tyr Thr Lys Leu Glu Glu Lys Ile Ala Ala Glu
370 375 380Lys Arg Glu Gln Glu Leu Ala Ala Glu Lys Glu Ala Ala Glu Lys Arg385 390 395 400Glu Leu Glu Leu Ala Ala Ala Lys Glu Ala Glu Asp Ala Ala Glu Ala
405 410 415Leu His Leu Ala Ala Glu Arg Ala Ser Thr Gln Glu Ala Glu Leu Ala
420 425 430His Gln His Gly Ala Asp Ala Ile Ala Asp Glu Val Ala Glu Glu Pro
435 440 445Gln Glu Leu Pro Gln Asp Glu Val Ala Ala Glu Val Glu Thr Glu Pro
450 455 460Asp Thr Glu Pro Asp Thr Glu Ser Asp Ser Glu Gln Ala Glu Val Ala465 470 475 480Ser Glu Glu Pro Glu Ala Asp Glu Glu Glu Lys
485 490<210>367<211>547<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(547)<223>RXA01111<400>367agaaaaccct gccgatgcaa actttgagga gagattcaat caaggaatag aaatcattct 60ggtgggtcta gacgcgcttg ggcatataag atgacgttcc atg aca tca acg att 115
Met Thr Ser Thr Ile
1 5gag cac tcg tac caa gtt tgg cct gga cat gct tat cct ctg ggt tca 163Glu His Ser Tyr Gln Val Trp Pro Gly His Ala Tyr Pro Leu Gly Ser
10 15 20acc tat gac ggt gct gga acg aac ttc gca ctc ttc tcc gac gtt gca 211Thr Tyr Asp Gly Ala Gly Thr Asn Phe Ala Leu Phe Ser Asp Val Ala
25 30 35gag cgt gtt gag ctg tgt cta tta gat gca gat aac aac gag act cga 259Glu Arg Val Glu Leu Cys Leu Leu Asp Ala Asp Asn Asn Glu Thr Arg
40 45 50att cca ctc gaa gag cgc gat gcc cac att tgg cat tgc tac ctt cct 307Ile Pro Leu Glu Glu Arg Asp Ala His Ile Trp His Cys Tyr Leu Pro
55 60 65ggc gtt caa cct gga cag cgc tac gga ttc cga gtt cat ggc ccg tgg 355Gly Val Gln Pro Gly Gln Arg Tyr Gly Phe Arg Val His Gly Pro Trp70 75 80 85aac cca gat gag ggt aag cgg tgc gac gcg aac aaa ctt cta gtt gat 403Asn Pro Asp Glu Gly Lys Arg Cys Asp Ala Asn Lys Leu Leu Val Asp
90 95 100ccc tat gct cgt gct ttc gat gga gat ttt gat gga cat ccg tca cta 451Pro Tyr Ala Arg Ala Phe Asp Gly Asp Phe Asp Gly His Pro Ser Leu
105 110 115ttt tct tac gac atc acc aat cca aat gac ccc aac ggt cgc aat acc 499Phe Ser Tyr Asp Ile Thr Asn Pro Asn Asp Pro Asn Gly Arg Asn Thr
120 125 130gaa gac agc att gat cac aca atg aag tct gtc gtg gtg aac cca ttc 547Glu Asp Ser Ile Asp His Thr Met Lys Ser Val Val Val Asn Pro Phe
135 140 145<210>368<211>149<212>PRT<213>谷氨酸棒杆菌<400>368Met Thr Ser Thr Ile Glu His Ser Tyr Gln Val Trp Pro Gly His Ala1 5 10 15Tyr Pro Leu Gly Ser Thr Tyr Asp Gly Ala Gly Thr Asn Phe Ala Leu
20 25 30Phe Ser Asp Val Ala Glu Arg Val Glu Leu Cys Leu Leu Asp Ala Asp
35 40 45Asn Asn Glu Thr Arg Ile Pro Leu Glu Glu Arg Asp Ala His Ile Trp
50 55 60His Cys Tyr Leu Pro Gly Val Gln Pro Gly Gln Arg Tyr Gly Phe Arg65 70 75 80Val His Gly Pro Trp Asn Pro Asp Glu Gly Lys Arg Cys Asp Ala Asn
85 90 95Lys Leu Leu Val Asp Pro Tyr Ala Arg Ala Phe Asp Gly Asp Phe Asp
100 105 110Gly His Pro Ser Leu Phe Ser Tyr Asp Ile Thr Asn Pro Asn Asp Pro
115 120 125Asn Gly Arg Asn Thr Glu Asp Ser Ile Asp His Thr Met Lys Ser Val
130 135 140Val Val Asn Pro Phe145<210>369<211>1635<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1612)<223>RXN01550<400>369ttcgccagca gtacttcttc acctctgctt ccctgcaggc catgattcag ggccacctgg 60cgcaccacaa ggacctcagc aactttgccg agttcactcc gtg cag ctc aat gac 115
Val Gln Leu Asn Asp
1 5act cac cca gtg ttg gct atc cct gag ctt atg cgt ctg ctc atg gac 163Thr His Pro Val Leu Ala Ile Pro Glu Leu Met Arg Leu Leu Met Asp
10 15 20gag cat gac atg ggc tgg gaa gaa tcc tgg gca atc gtg ttc aag acc 211Glu His Asp Met Gly Trp Glu Glu Ser Trp Ala Ile Val Phe Lys Thr
25 30 35ttc gca tac acc aac cac acc gtg ctc acc gaa gct ctt gag cag tgg 259Phe Ala Tyr Thr Asn His Thr Val Leu Thr Glu Ala Leu Glu Gln Trp
40 45 50gat cag cag atc ttc caa cag ctg ttc tgg cgc gtg tgg gaa atc atc 307Asp Gln Gln Ile Phe Gln Gln Leu Phe Trp Arg Val Trp Glu Ile Ile
55 60 65aca gag atc gat cgc cgc ttc cgt ttg gag cgc gca gcc gat gga ctg 355Thr Glu Ile Asp Arg Arg Phe Arg Leu Glu Arg Ala Ala Asp Gly Leu70 75 80 85gat gaa gag acc atc gac cgc atg gct cca atc cag cac ggc act gtt 403Asp Glu Glu Thr Ile Asp Arg Met Ala Pro Ile Gln His Gly Thr Val
90 95 100cat atg gca tgg att gcc tgt tac gcg gca tat tcc atc aat ggc gtg 451His Met Ala Trp Ile Ala Cys Tyr Ala Ala Tyr Ser Ile Asn Gly Val
105 110 115gca gcg ctg cac acc gag atc atc aag gcc gag acc ttg gct gac tgg 499Ala Ala Leu His Thr Glu Ile Ile Lys Ala Glu Thr Leu Ala Asp Trp
120 125 130tac gca ctg tgg cca gag aag ttc aac aac aag act aac ggt gtt acc 547Tyr Ala Leu Trp Pro Glu Lys Phe Asn Asn Lys Thr Asn Gly Val Thr
135 140 145cca cgc cgt tgg ctg cgc atg atc aac cca ggt ctg tct gac ctg ctc 595Pro Arg Arg Trp Leu Arg Met Ile Asn Pro Gly Leu Ser Asp Leu Leu150 155 160 165act cga ctt tcc ggt tcc gat gat tgg gta acc gat ctg gat gag ctg 643Thr Arg Leu Ser Gly Ser Asp Asp Trp Val Thr Asp Leu Asp Glu Leu
170 175 180aag aag ctg cgc tcc tat gcc gac gat aag tcc gtg ctt gaa gaa ctc 691Lys Lys Leu Arg Ser Tyr Ala Asp Asp Lys Ser Val Leu Glu Glu Leu
185 190 195cgc gct atc aag gct gct aat aag caa gac ttc gcc gag tgg atc ctc 739Arg Ala Ile Lys Ala Ala Asn Lys Gln Asp Phe Ala Glu Trp Ile Leu
200 205 210gag cgc cag ggc att gag att gat cca gaa tcc atc ttt gac gtg cag 787Glu Arg Gln Gly Ile Glu Ile Asp Pro Glu Ser Ile Phe Asp Val Gln
215 220 225att aag cgc ctc cac gag tac aag cgc cag ctc atg aac gcg ctc tac 835Ile Lys Arg Leu His Glu Tyr Lys Arg Gln Leu Met Asn Ala Leu Tyr230 235 240 245gta cta gac ctt tac ttc cgt att aag gaa gat ggc ctc acc gac atc 883Val Leu Asp Leu Tyr Phe Arg Ile Lys Glu Asp Gly Leu Thr Asp Ile
250 255 260cca gca cgc act gtc atc ttt ggc gcc aag gcc gcg ccg ggt tat gtc 931Pro Ala Arg Thr Val Ile Phe Gly Ala Lys Ala Ala Pro Gly Tyr Val
265 270 275cgc gcc aag gcg att atc aag ctc atc aac tct att gct gac ttg gta 979Arg Ala Lys Ala Ile Ile Lys Leu Ile Asn Ser Ile Ala Asp Leu Val
280 285 290aac aac gat cct gag gtc tcc ccg ctg ctc aag gtg gtc ttt gta gag 1027Asn Asn Asp Pro Glu Val Ser Pro Leu Leu Lys Val Val Phe Val Glu
295 300 305aac tac aac gtc tcc cct gct gag cac atc ttg cct gcg tct gat gtc 1075Asn Tyr Asn Val Ser Pro Ala Glu His Ile Leu Pro Ala Ser Asp Val310 315 320 325tcc gaa cag att tcc acc gcc ggc aag gaa gcc agc ggt acc tcc aac 1123Ser Glu Gln Ile Ser Thr Ala Gly Lys Glu Ala Ser Gly Thr Ser Asn
330 335 340atg aag ttc atg atg aac ggc gcc ctc acc ctg ggc acc atg gac ggc 1171Met Lys Phe Met Met Asn Gly Ala Leu Thr Leu Gly Thr Met Asp Gly
345 350 355gcc aac gta gag atc gtg gat tct gtg ggc gag gaa aac gcc tat atc 1219Ala Asn Val Glu Ile Val Asp Ser Val Gly Glu Glu Asn Ala Tyr Ile
360 365 370ttc ggt gct cgc gtg gaa gaa ttg cca gcc ctg cgc gaa agc tac gag 1267Phe Gly Ala Arg Val Glu Glu Leu Pro Ala Leu Arg Glu Ser Tyr Glu
375 380 385cca tat gag ctc tat gag acc gtc cct ggc ctc aag cgc gca ttg gac 1315Pro Tyr Glu Leu Tyr Glu Thr Val Pro Gly Leu Lys Arg Ala Leu Asp390 395 400 405gcc ctg gat aac ggc acc ctc aac gac aac aac agt ggt ttg ttc tac 1363Ala Leu Asp Asn Gly Thr Leu Asn Asp Asn Asn Ser Gly Leu Phe Tyr
410 415 420gac ctc aag cat tcc ttg atc cac ggt tat gga aaa gac gcc agc gac 1411Asp Leu Lys His Ser Leu Ile His Gly Tyr Gly Lys Asp Ala Ser Asp
425 430 435acc tac tac gtg ctt ggc gat ttc gca gat tac cgc gag acc cgc gac 1459Thr Tyr Tyr Val Leu Gly Asp Phe Ala Asp Tyr Arg Glu Thr Arg Asp
440 445 450cgt atg gcc gcc gac tac gcc tcc gat ccc ctg ggt tgg gca cgc atg 1507Arg Met Ala Ala Asp Tyr Ala Ser Asp Pro Leu Gly Trp Ala Arg Met
455 460 465gcc tgg atc aac att tgc gag tcc ggc cgt ttc tcc tcc gac cgc acc 1555Ala Trp Ile Asn Ile Cys Glu Ser Gly Arg Phe Ser Ser Asp Arg Thr470 475 480 485atc cgc gat tat gcc acc gag atc tgg aag ctc gag cca act cct gct 1603Ile Arg Asp Tyr Ala Thr Glu Ile Trp Lys Leu Glu Pro Thr Pro Ala
490 495 500gtt aag aag taggttttaa cctccgcttc taa 1635Val Lys Lys<210>370<211>504<212>PRT<213>谷氨酸棒杆菌<400>370Val Gln Leu Asn Asp Thr His Pro Val Leu Ala Ile Pro Glu Leu Met1 5 10 15Arg Leu Leu Met Asp Glu His Asp Met Gly Trp Glu Glu Ser Trp Ala
20 25 30Ile Val Phe Lys Thr Phe Ala Tyr Thr Asn His Thr Val Leu Thr Glu
35 40 45Ala Leu Glu Gln Trp Asp Gln Gln Ile Phe Gln Gln Leu Phe Trp Arg
50 55 60Val Trp Glu Ile Ile Thr Glu Ile Asp Arg Arg Phe Arg Leu Glu Arg65 70 75 80Ala Ala Asp Gly Leu Asp Glu Glu Thr Ile Asp Arg Met Ala Pro Ile
85 90 95Gln His Gly Thr Val His Met Ala Trp Ile Ala Cys Tyr Ala Ala Tyr
100 105 110Ser Ile Asn Gly Val Ala Ala Leu His Thr Glu Ile Ile Lys Ala Glu
115 120 125Thr Leu Ala Asp Trp Tyr Ala Leu Trp Pro Glu Lys Phe Asn Asn Lys
130 135 140Thr Asn Gly Val Thr Pro Arg Arg Trp Leu Arg Met Ile Asn Pro Gly145 150 155 160Leu Ser Asp Leu Leu Thr Arg Leu Ser Gly Ser Asp Asp Trp Val Thr
165 170 175Asp Leu Asp Glu Leu Lys Lys Leu Arg Ser Tyr Ala Asp Asp Lys Ser
180 185 190Val Leu Glu Glu Leu Arg Ala Ile Lys Ala Ala Asn Lys Gln Asp Phe
195 200 205Ala Glu Trp Ile Leu Glu Arg Gln Gly Ile Glu Ile Asp Pro Glu Ser
210 215 220Ile Phe Asp Val Gln Ile Lys Arg Leu His Glu Tyr Lys Arg Gln Leu225 230 235 240Met Asn Ala Leu Tyr Val Leu Asp Leu Tyr Phe Arg Ile Lys Glu Asp
245 250 255Gly Leu Thr Asp Ile Pro Ala Arg Thr Val Ile Phe Gly Ala Lys Ala
260 265 270Ala Pro Gly Tyr Val Arg Ala Lys Ala Ile Ile Lys Leu Ile Asn Ser
275 280 285Ile Ala Asp Leu Val Asn Asn Asp Pro Glu Val Ser Pro Leu Leu Lys
290 295 300Val Val Phe Val Glu Asn Tyr Asn Val Ser Pro Ala Glu His Ile Leu305 310 315 320Pro Ala Ser Asp Val Ser Glu Gln Ile Ser Thr Ala Gly Lys Glu Ala
325 330 335Ser Gly Thr Ser Asn Met Lys Phe Met Met Asn Gly Ala Leu Thr Leu
340 345 350Gly Thr Met Asp Gly Ala Asn Val Glu Ile Val Asp Ser Val Gly Glu
355 360 365Glu Asn Ala Tyr Ile Phe Gly Ala Arg Val Glu Glu Leu Pro Ala Leu
370 375 380Arg Glu Ser Tyr Glu Pro Tyr Glu Leu Tyr Glu Thr Val Pro Gly Leu385 390 395 400Lys Arg Ala Leu Asp Ala Leu Asp Asn Gly Thr Leu Asn Asp Asn Asn
405 410 415Ser Gly Leu Phe Tyr Asp Leu Lys His Ser Leu Ile His Gly Tyr Gly
420 425 430Lys Asp Ala Ser Asp Thr Tyr Tyr Val Leu Gly Asp Phe Ala Asp Tyr
435 440 445Arg Glu Thr Arg Asp Arg Met Ala Ala Asp Tyr Ala Ser Asp Pro Leu
450 455 460Gly Trp Ala Arg Met Ala Trp Ile Asn Ile Cys Glu Ser Gly Arg Phe465 470 475 480Ser Ser Asp Arg Thr Ile Arg Asp Tyr Ala Thr Glu Ile Trp Lys Leu
485 490 495Glu Pro Thr Pro Ala Val Lys Lys
500<210>371<211>1367<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1344)<223>FRXA01550<400>371atc ttc caa cag ctg ttc tgg cgc gtg tgg gaa atc atc aca gag atc 48Ile Phe Gln Gln Leu Phe Trp Arg Val Trp Glu Ile Ile Thr Glu Ile1 5 10 15gat cgc cgc ttc cgt ttg gag cgc gca gcc gat gga ctg gat gaa gag 96Asp Arg Arg Phe Arg Leu Glu Arg Ala Ala Asp Gly Leu Asp Glu Glu
20 25 30acc atc gac cgc atg gct cca atc cag cgc ggc act gtt cat atg gca 144Thr Ile Asp Arg Met Ala Pro Ile Gln Arg Gly Thr Val His Met Ala
35 40 45tgg att gcc tgt tac gcg gca tat tcc atc aat ggc gtg gca gcg ctg 192Trp Ile Ala Cys Tyr Ala Ala Tyr Ser Ile Asn Gly Val Ala Ala Leu
50 55 60cac acc gag atc atc aag gcc gag acc ttg gct gac tgg tac gca ctg 240His Thr Glu Ile Ile Lys Ala Glu Thr Leu Ala Asp Trp Tyr Ala Leu65 70 75 80tgg cca gag aag ttc aac aac aag act aac ggt gtt acc cca cgc cgt 288Trp Pro Glu Lys Phe Asn Asn Lys Thr Asn Gly Val Thr Pro Arg Arg
85 90 95tgg ctg cgc atg atc aac cca ggt ctg tct gac ctg ctc act cga ctt 336Trp Leu Arg Met Ile Asn Pro Gly Leu Ser Asp Leu Leu Thr Arg Leu
100 105 110tcc ggt tcc gat gat tgg gta acc gat ctg gat gag ctg aag aag ctg 384Ser Gly Ser Asp Asp Trp Val Thr Asp Leu Asp Glu Leu Lys Lys Leu
115 120 125cgc tcc tat gcc gac gat aag tcc gtg ctt gaa gaa ctc cgc gct atc 432Arg Ser Tyr Ala Asp Asp Lys Ser Val Leu Glu Glu Leu Arg Ala Ile
130 135 140aag gct gct aat aag caa gac ttc gcc gag tgg atc ctc gag cgc cag 480Lys Ala Ala Asn Lys Gln Asp Phe Ala Glu Trp Ile Leu Glu Arg Gln145 150 155 160ggc att gag att gat cca gaa tcc atc ttt gac gtg cag att aag cgc 528Gly Ile Glu Ile Asp Pro Glu Ser Ile Phe Asp Val Gln Ile Lys Arg
165 170 175ctc cac gag tac aag cgc cag ctc atg aac gcg ctc tac gta cta gac 576Leu His Glu Tyr Lys Arg Gln Leu Met Asn Ala Leu Tyr Val Leu Asp
180 185 190ctt tac ttc cgt att aag gaa gat ggc ctc acc gac atc cca gca cgc 624Leu Tyr Phe Arg Ile Lys Glu Asp Gly Leu Thr Asp Ile Pro Ala Arg
195 200 205act gtc atc ttt ggc gcc aag gcc gcg ccg ggt tat gtc cgc gcc aag 672Thr Val Ile Phe Gly Ala Lys Ala Ala Pro Gly Tyr Val Arg Ala Lys
210 215 220gcg att atc aag ctc atc aac tct att gct gac ttg gta aac aac gat 720Ala Ile Ile Lys Leu Ile Asn Ser Ile Ala Asp Leu Val Asn Asn Asp225 230 235 240cct gag gtc tcc ccg ctg ctc aag gtg gtc ttt gta gag aac tac aac 768Pro Glu Val Ser Pro Leu Leu Lys Val Val Phe Val Glu Asn Tyr Asn
245 250 255gtc tcc cct gct gag cac atc ttg cct gcg tct gat gtc tcc gaa cag 816Val Ser Pro Ala Glu His Ile Leu Pro Ala Ser Asp Val Ser Glu Gln
260 265 270att tcc acc gcc ggc aag gaa gcc agc ggt acc tcc aac atg aag ttc 864Ile Ser Thr Ala Gly Lys Glu Ala Ser Gly Thr Ser Asn Met Lys Phe
275 280 285atg atg aac ggc gcc ctc acc ctg ggc acc atg gac ggc gcc aac gta 912Met Met Asn Gly Ala Leu Thr Leu Gly Thr Met Asp Gly Ala Asn Val
290 295 300gag atc gtg gat tct gtg ggc gag gaa aac gcc tat atc ttc ggt gct 960Glu Ile Val Asp Ser Val Gly Glu Glu Asn Ala Tyr Ile Phe Gly Ala305 310 315 320cgc gtg gaa gaa ttg cca gcc ctg cgc gaa agc tac gag cca tat gag 1008Arg Val Glu Glu Leu Pro Ala Leu Arg Glu Ser Tyr Glu Pro Tyr Glu
325 330 335ctc tat gag acc gtc cct ggc ctc aag cgc gca ttg gac gcc ctg gat 1056Leu Tyr Glu Thr Val Pro Gly Leu Lys Arg Ala Leu Asp Ala Leu Asp
340 345 350aac ggc acc ctc aac gac aac aac agt ggt ttg ttc tac gac ctc aag 1104Asn Gly Thr Leu Asn Asp Asn Asn Ser Gly Leu Phe Tyr Asp Leu Lys
355 360 365cat tcc ttg atc cac ggt tat gga aaa gac gcc agc gac acc tac tac 1152His Ser Leu Ile His Gly Tyr Gly Lys Asp Ala Ser Asp Thr Tyr Tyr
370 375 380gtg ctt ggc gat ttc gca gat tac cgc gag acc cgc gac cgt atg gcc 1200Val Leu Gly Asp Phe Ala Asp Tyr Arg Glu Thr Arg Asp Arg Met Ala385 390 395 400gcc gac tac gcc tcc gat ccc ctg ggt tgg gca cgc atg gcc tgg atc 1248Ala Asp Tyr Ala Ser Asp Pro Leu Gly Trp Ala Arg Met Ala Trp Ile
405 410 415aac att tgc gag tcc ggc cgt ttc tcc tcc gac cgc acc atc cgc gat 1296Asn Ile Cys Glu Ser Gly Arg Phe Ser Ser Asp Arg Thr Ile Arg Asp
420 425 430tat gcc acc gag atc tgg aag ctc gag cca act cct gct gtt aag aag 1344Tyr Ala Thr Glu Ile Trp Lys Leu Glu Pro Thr Pro Ala Val Lys Lys
435 440 445taggttttaa cctccgcttc taa 1367<210>372<211>448<212>PRT<213>谷氨酸棒杆菌<400>372Ile Phe Gln Gln Leu Phe Trp Arg Val Trp Glu Ile Ile Thr Glu Ile1 5 10 15Asp Arg Arg Phe Arg Leu Glu Arg Ala Ala Asp Gly Leu Asp Glu Glu
20 25 30Thr Ile Asp Arg Met Ala Pro Ile Gln Arg Gly Thr Val His Met Ala
35 40 45Trp Ile Ala Cys Tyr Ala Ala Tyr Ser Ile Asn Gly Val Ala Ala Leu
50 55 60His Thr Glu Ile Ile Lys Ala Glu Thr Leu Ala Asp Trp Tyr Ala Leu65 70 75 80Trp Pro Glu Lys Phe Asn Asn Lys Thr Asn Gly Val Thr Pro Arg Arg
85 90 95Trp Leu Arg Met Ile Asn Pro Gly Leu Ser Asp Leu Leu Thr Arg Leu
100 105 110Ser Gly Ser Asp Asp Trp Val Thr Asp Leu Asp Glu Leu Lys Lys Leu
115 120 125Arg Ser Tyr Ala Asp Asp Lys Ser Val Leu Glu Glu Leu Arg Ala Ile
130 135 140Lys Ala Ala Asn Lys Gln Asp Phe Ala Glu Trp Ile Leu Glu Arg Gln145 150 155 160Gly Ile Glu Ile Asp Pro Glu Ser Ile Phe Asp Val Gln Ile Lys Arg
165 170 175Leu His Glu Tyr Lys Arg Gln Leu Met Asn Ala Leu Tyr Val Leu Asp
180 185 190Leu Tyr Phe Arg Ile Lys Glu Asp Gly Leu Thr Asp Ile Pro Ala Arg
195 200 205Thr Val Ile Phe Gly Ala Lys Ala Ala Pro Gly Tyr Val Arg Ala Lys
210 215 220Ala Ile Ile Lys Leu Ile Asn Ser Ile Ala Asp Leu Val Asn Asn Asp225 230 235 240Pro Glu Val Ser Pro Leu Leu Lys Val Val Phe Val Glu Asn Tyr Asn
245 250 255Val Ser Pro Ala Glu His Ile Leu Pro Ala Ser Asp Val Ser Glu Gln
260 265 270Ile Ser Thr Ala Gly Lys Glu Ala Ser Gly Thr Ser Asn Met Lys Phe
275 280 285Met Met Asn Gly Ala Leu Thr Leu Gly Thr Met Asp Gly Ala Asn Val
290 295 300Glu Ile Val Asp Ser Val Gly Glu Glu Asn Ala Tyr Ile Phe Gly Ala305 310 315 320Arg Val Glu Glu Leu Pro Ala Leu Arg Glu Ser Tyr Glu Pro Tyr Glu
325 330 335Leu Tyr Glu Thr Val Pro Gly Leu Lys Arg Ala Leu Asp Ala Leu Asp
340 345 350Asn Gly Thr Leu Asn Asp Asn Asn Ser Gly Leu Phe Tyr Asp Leu Lys
355 360 365His Ser Leu Ile His Gly Tyr Gly Lys Asp Ala Ser Asp Thr Tyr Tyr
370 375 380Val Leu Gly Asp Phe Ala Asp Tyr Arg Glu Thr Arg Asp Arg Met Ala385 390 395 400Ala Asp Tyr Ala Ser Asp Pro Leu Gly Trp Ala Arg Met Ala Trp Ile
405 410 415Asn Ile Cys Glu Ser Gly Arg Phe Ser Ser Asp Arg Thr Ile Arg Asp
420 425 430Tyr Ala Thr Glu Ile Trp Lys Leu Glu Pro Thr Pro Ala Val Lys Lys
435 440 445<210>373<211>2348<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2325)<223>RXN02100<400>373cta ggt cga atc aac gcc gag gag caa aac ctc agc gaa tac ctc agc 48Leu Gly Arg Ile Asn Ala Glu Glu Gln Asn Leu Ser Glu Tyr Leu Ser1 5 10 15gac aag ctg tgg tac cag gac acc gca gat gca acc gat gct gtc gga 96Asp Lys Leu Trp Tyr Gln Asp Thr Ala Asp Ala Thr Asp Ala Val Gly
20 25 30gat cca ctc gtt gcg tac ttc tcc atg gag ttt ggc att cac cca agc 144Asp Pro Leu Val Ala Tyr Phe Ser Met Glu Phe Gly Ile His Pro Ser
35 40 45ctg cca atc tac tct ggc gga ctt ggt gtg ctt gcg ggc gag aac atg 192Leu Pro Ile Tyr Ser Gly Gly Leu Gly Val Leu Ala Gly Glu Asn Met
50 55 60aag tct gca tct gac ttg ggt gtg cca ctg atc ggt gtt ggt ttg ctc 240Lys Ser Ala Ser Asp Leu Gly Val Pro Leu Ile Gly Val Gly Leu Leu65 70 75 80tac acc cac ggc tac ttc acc cag tca ctg tcc ggt gac ggt tgg cag 288Tyr Thr His Gly Tyr Phe Thr Gln Ser Leu Ser Gly Asp Gly Trp Gln
85 90 95cag gaa gag tac aag tac cac gat cca gca gaa ctg ccg att gag gca 336Gln Glu Glu Tyr Lys Tyr His Asp Pro Ala Glu Leu Pro Ile Glu Ala
100 105 110gtt aaa gat aag aac ggc gag cag gtc act gtt tct gtc acc tac cca 384Val Lys Asp Lys Asn Gly Glu Gln Val Thr Val Ser Val Thr Tyr Pro
115 120 125ggt gcg cag gaa gta aag att gca ctg tgg gta gca aac gtt ggc cgc 432Gly Ala Gln Glu Val Lys Ile Ala Leu Trp Val Ala Asn Val Gly Arg
130 135 140atc cca ttg ctg ctg ctt gat acc aac atc gag gca aac cca gaa gag 480Ile Pro Leu Leu Leu Leu Asp Thr Asn Ile Glu Ala Asn Pro Glu Glu145 150 155 160ctc cgc aac gtt act gac cgc ctg tac ggt ggc gac aat gag cac cgc 528Leu Arg Asn Val Thr Asp Arg Leu Tyr Gly Gly Asp Asn Glu His Arg
165 170 175atc aag cag gaa ctc gtt ctc ggt gtt ggt ggc gtc cgc gct gtc aac 576Ile Lys Gln Glu Leu Val Leu Gly Val Gly Gly Val Arg Ala Val Asn
180 185 190gca ttc tgc gaa gct cgt ggt ctg aag cgc tca tct gtt gca cac ctc 624Ala Phe Cys Glu Ala Arg Gly Leu Lys Arg Ser Ser Val Ala His Leu
195 200 205aac gaa ggc cac gca ggt ttc ctg acc ctg gag cgt atc cgc gag cgc 672Asn Glu Gly His Ala Gly Phe Leu Thr Leu Glu Arg Ile Arg Glu Arg
210 215 220atc gca gag ggc atg gag tac cca gca gca ttc gag cag gtt cgt gcg 720Ile Ala Glu Gly Met Glu Tyr Pro Ala Ala Phe Glu Gln Val Arg Ala225 230 235 240tcc aac atc ttc acc acc cac acc cca gtc cca gca ggc atc gac cgc 768Ser Asn Ile Phe Thr Thr His Thr Pro Val Pro Ala Gly Ile Asp Arg
245 250 255ttc gac atg gag atg gtg cgt cgt tat ctc ggt ggc ggt cag cca gaa 816Phe Asp Met Glu Met Val Arg Arg Tyr Leu Gly Gly Gly Gln Pro Glu
260 265 270gat cag cag ctg tgc gtt ggt gtt cca att gag aag gca ctt gag ctt 864Asp Gln Gln Leu Cys Val Gly Val Pro Ile Glu Lys Ala Leu Glu Leu
275 280 285ggt caa gag tcc gat cca cac cgc ttc aac atg gct cat atg ggc ctt 912Gly Gln Glu Ser Asp Pro His Arg Phe Asn Met Ala His Met Gly Leu
290 295 300cgc gcg agc caa cat gct aat ggc gtc gca aag ctt cat ggt gaa gta 960Arg Ala Ser Gln His Ala Asn Gly Val Ala Lys Leu His Gly Glu Val305 310 315 320agc cgt gac atg ttc gcc ggc ctg tac ccc gga tat gag cct cgt gaa 1008Ser Arg Asp Met Phe Ala Gly Leu Tyr Pro Gly Tyr Glu Pro Arg Glu
325 330 335
gtg ccc atc ggg cac gtc acc aac ggt gtt cac ctg ccg acg tgg gtc 1056Val Pro Ile Gly His Val Thr Asn Gly Val His Leu Pro Thr Trp Val
340 345 350aag cca gag atg aag gaa ctc atc gat cgc gtc act ggc ggc gct gat 1104Lys Pro Glu Met Lys Glu Leu Ile Asp Arg Val Thr Gly Gly Ala Asp
355 360 365ctt gcg gtt gct gat tct tgg tca aac cca cag gct gtc gag tct gag 1152Leu Ala Val Ala Asp Ser Trp Ser Asn Pro Gln Ala Val Glu Ser Glu
370 375 380aag atc tgg aag gtg cgc aac aag ttc cgt gct gac cta gtg gag gtt 1200Lys Ile Trp Lys Val Arg Asn Lys Phe Arg Ala Asp Leu Val Glu Val385 390 395 400gct cgc gct gca act gca aag tcc tgg tct cac cgt gga cac acc gaa 1248Ala Arg Ala Ala Thr Ala Lys Ser Trp Ser His Arg Gly His Thr Glu
405 410 415gca gaa ctt gcg tgg acc tcc cgc gtt ctg gat cca aac gtg ctg acc 1296Ala Glu Leu Ala Trp Thr Ser Arg Val Leu Asp Pro Asn Val Leu Thr
420 425 430att ggt ttc gca cgt cgc gta tcc acc tac aag cgc ttg acc ttg atg 1344Ile Gly Phe Ala Arg Arg Val Ser Thr Tyr Lys Arg Leu Thr Leu Met
435 440 445ctg cgc aac cct gaa cgc ctg cgt tcc atc ttg ctt aat gag gaa cgc 1392Leu Arg Asn Pro Glu Arg Leu Arg Ser Ile Leu Leu Asn Glu Glu Arg
450 455 460cca gtt cag ttc gtt att gct ggt aag gca cac cca cat gac atg ggt 1440Pro Val Gln Phe Val Ile Ala Gly Lys Ala His Pro His Asp Met Gly465 470 475 480ggc aag aag ctc atg cag gaa atc gtc cac ttc gct gat caa gct ggt 1488Gly Lys Lys Leu Met Gln Glu Ile Val His Phe Ala Asp Gln Ala Gly
485 490 495gtc cgt gac cgt ttc ctc ttc ctg cct gat tac gac atc aac ctg gcc 1536Val Arg Asp Arg Phe Leu Phe Leu Pro Asp Tyr Asp Ile Asn Leu Ala
500 505 510agc tac ctg atc tct ggt gct gac gtg tgg ctg aac aac cca gtg cgc 1584Ser Tyr Leu Ile Ser Gly Ala Asp Val Trp Leu Asn Asn Pro Val Arg
515 520 525cct cag gaa gca tcg gga acc tcc ggt atg aag gcc gtc atg aat ggt 1632Pro Gln Glu Ala Ser Gly Thr Ser Gly Met Lys Ala Val Met Asn Gly
530 535 540ggc ctg acc ctg tcc atc tct gat ggt tgg tgg gat gaa atg cct aag 1680Gly Leu Thr Leu Ser Ile Ser Asp Gly Trp Trp Asp Glu Met Pro Lys545 550 555 560gag acc acc ggc tgg acc atc cca acc gtt gag tcc cag gac ttg gaa 1728Glu Thr Thr Gly Trp Thr Ile Pro Thr Val Glu Ser Gln Asp Leu Glu
565 570 575tgc cgc gac cac ctg gaa tcc cag gcg ctg tac gac ctg ctg gaa aac 1776Cys Arg Asp His Leu Glu Ser Gln Ala Leu Tyr Asp Leu Leu Glu Asn
580 585 590gaa gtt gca ccg ctg ttt tac aag cgc gac aag aac ggc atc cca cag 1824Glu Val Ala Pro Leu Phe Tyr Lys Arg Asp Lys Asn Gly Ile Pro Gln
595 600 605gac tgg ctg gac ctg gtt cgc gaa tcc tgg acc acc ctg tca cca atg 1872Asp Trp Leu Asp Leu Val Arg Glu Ser Trp Thr Thr Leu Ser Pro Met
610 615 620gtc acc tcc acc cgc atg gtg cgc gac tac acc acc cag tac tac cgc 1920Val Thr Ser Thr Arg Met Val Arg Asp Tyr Thr Thr Gln Tyr Tyr Arg625 630 635 640cca acc aaa cac cag gca gag ctc att gcg cag cct gca gaa gca gcg 1968Pro Thr Lys His Gln Ala Glu Leu Ile Ala Gln Pro Ala Glu Ala Ala
645 650 655gat tac gcg gca tgg ctt gag cac atc aaa gca gag tgg gct ggc gtc 2016Asp Tyr Ala Ala Trp Leu Glu His Ile Lys Ala Glu Trp Ala Gly Val
660 665 670aag gtc tca gac ctg aag atc agc gag agc gcc atc acg gcg cag gag 2064Lys Val Ser Asp Leu Lys Ile Ser Glu Ser Ala Ile Thr Ala Gln Glu
675 680 685ctt gaa gtc agc gtt cgc gtt gat tcc ggt tcg ctt aac gac gac gag 2112Leu Glu Val Ser Val Arg Val Asp Ser Gly Ser Leu Asn Asp Asp Glu
690 695 700ttc caa gct cag gca ctc ttt ggt gcg ctc gga cac aac ggt gac atc 2160Phe Gln Ala Gln Ala Leu Phe Gly Ala Leu Gly His Asn Gly Asp Ile705 710 715 720gaa gat cca gaa atc acc gtt ttg acc cca cgc ggc gat ggc gcc tac 2208Glu Asp Pro Glu Ile Thr Val Leu Thr Pro Arg Gly Asp Gly Ala Tyr
725 730 735gcg gca aag gtc agc act gac ctg cca ggc aac tac ggc atc act gcc 2256Ala Ala Lys Val Ser Thr Asp Leu Pro Gly Asn Tyr Gly Ile Thr Ala
740 745 750cgc gtt gtt cca aac aac agg atg ctg gtc agc cca gcg gaa acc cgc 2304Arg Val Val Pro Asn Asn Arg Met Leu Val Ser Pro Ala Glu Thr Arg
755 760 765ctg atc acc tac ttg gag aac tagggcgaaa ctagctttac caa 2348Leu Ile Thr Tyr Leu Glu Asn
770 775<210>374<211>775<212>PRT<213>谷氨酸棒杆菌<400>374Leu Gly Arg Ile Asn Ala Glu Glu Gln Asn Leu Ser Glu Tyr Leu Ser1 5 10 15Asp Lys Leu Trp Tyr Gln Asp Thr Ala Asp Ala Thr Asp Ala Val Gly
20 25 30Asp Pro Leu Val Ala Tyr Phe Ser Met Glu Phe Gly Ile His Pro Ser
35 40 45Leu Pro Ile Tyr Ser Gly Gly Leu Gly Val Leu Ala Gly Glu Asn Met
50 55 60Lys Ser Ala Ser Asp Leu Gly Val Pro Leu Ile Gly Val Gly Leu Leu65 70 75 80Tyr Thr His Gly Tyr Phe Thr Gln Ser Leu Ser Gly Asp Gly Trp Gln
85 90 95Gln Glu Glu Tyr Lys Tyr His Asp Pro Ala Glu Leu Pro Ile Glu Ala
100 105 110Val Lys Asp Lys Asn Gly Glu Gln Val Thr Val Ser Val Thr Tyr Pro
115 120 125Gly Ala Gln Glu Val Lys Ile Ala Leu Trp Val Ala Asn Val Gly Arg
130 135 140Ile Pro Leu Leu Leu Leu Asp Thr Asn Ile Glu Ala Asn Pro Glu Glu145 150 155 160Leu Arg Asn Val Thr Asp Arg Leu Tyr Gly Gly Asp Asn Glu His Arg
165 170 175Ile Lys Gln Glu Leu Val Leu Gly Val Gly Gly Val Arg Ala Val Asn
180 185 190Ala Phe Cys Glu Ala Arg Gly Leu Lys Arg Ser Ser Val Ala His Leu
195 200 205Asn Glu Gly His Ala Gly Phe Leu Thr Leu Glu Arg Ile Arg Glu Arg
210 215 220Ile Ala Glu Gly Met Glu Tyr Pro Ala Ala Phe Glu Gln Val Arg Ala225 230 235 240Ser Asn Ile Phe Thr Thr His Thr Pro Val Pro Ala Gly Ile Asp Arg
245 250 255Phe Asp Met Glu Met Val Arg Arg Tyr Leu Gly Gly Gly Gln Pro Glu
260 265 270Asp Gln Gln Leu Cys Val Gly Val Pro Ile Glu Lys Ala Leu Glu Leu
275 280 285Gly Gln Glu Ser Asp Pro His Arg Phe Asn Met Ala His Met Gly Leu
290 295 300Arg Ala Ser Gln His Ala Asn Gly Val Ala Lys Leu His Gly Glu Val305 310 315 320Ser Arg Asp Met Phe Ala Gly Leu Tyr Pro Gly Tyr Glu Pro Arg Glu
325 330 335Val Pro Ile Gly His Val Thr Asn Gly Val His Leu Pro Thr Trp Val
340 345 350Lys Pro Glu Met Lys Glu Leu Ile Asp Arg Val Thr Gly Gly Ala Asp
355 360 365Leu Ala Val Ala Asp Ser Trp Ser Asn Pro Gln Ala Val Glu Ser Glu
370 375 380Lys Ile Trp Lys Val Arg Asn Lys Phe Arg Ala Asp Leu Val Glu Val385 390 395 400Ala Arg Ala Ala Thr Ala Lys Ser Trp Ser His Arg Gly His Thr Glu
405 410 415Ala Glu Leu Ala Trp Thr Ser Arg Val Leu Asp Pro Asn Val Leu Thr
420 425 430Ile Gly Phe Ala Arg Arg Val Ser Thr Tyr Lys Arg Leu Thr Leu Met
435 440 445Leu Arg Asn Pro Glu Arg Leu Arg Ser Ile Leu Leu Asn Glu Glu Arg
450 455 460Pro Val Gln Phe Val Ile Ala Gly Lys Ala His Pro His Asp Met Gly465 470 475 480Gly Lys Lys Leu Met Gln Glu Ile Val His Phe Ala Asp Gln Ala Gly
485 490 495Val Arg Asp Arg Phe Leu Phe Leu Pro Asp Tyr Asp Ile Asn Leu Ala
500 505 510Ser Tyr Leu Ile Ser Gly Ala Asp Val Trp Leu Asn Asn Pro Val Arg
515 520 525Pro Gln Glu Ala Ser Gly Thr Ser Gly Met Lys Ala Val Met Asn Gly
530 535 540Gly Leu Thr Leu Ser Ile Ser Asp Gly Trp Trp Asp Glu Met Pro Lys545 550 555 560Glu Thr Thr Gly Trp Thr Ile Pro Thr Val Glu Ser Gln Asp Leu Glu
565 570 575Cys Arg Asp His Leu Glu Ser Gln Ala Leu Tyr Asp Leu Leu Glu Asn
580 585 590Glu Val Ala Pro Leu Phe Tyr Lys Arg Asp Lys Asn Gly Ile Pro Gln
595 600 605Asp Trp Leu Asp Leu Val Arg Glu Ser Trp Thr Thr Leu Ser Pro Met
610 615 620Val Thr Ser Thr Arg Met Val Arg Asp Tyr Thr Thr Gln Tyr Tyr Arg625 630 635 640Pro Thr Lys His Gln Ala Glu Leu Ile Ala Gln Pro Ala Glu Ala Ala
645 650 655Asp Tyr Ala Ala Trp Leu Glu His Ile Lys Ala Glu Trp Ala Gly Val
660 665 670Lys Val Ser Asp Leu Lys Ile Ser Glu Ser Ala Ile Thr Ala Gln Glu
675 680 685Leu Glu Val Ser Val Arg Val Asp Ser Gly Ser Leu Asn Asp Asp Glu
690 695 700Phe Gln Ala Gln Ala Leu Phe Gly Ala Leu Gly His Asn Gly Asp Ile705 710 715 720Glu Asp Pro Glu Ile Thr Val Leu Thr Pro Arg Gly Asp Gly Ala Tyr
725 730 735Ala Ala Lys Val Ser Thr Asp Leu Pro Gly Asn Tyr Gly Ile Thr Ala
740 745 750Arg Val Val Pro Asn Asn Arg Met Leu Val Ser Pro Ala Glu Thr Arg
755 760 765Leu Ile Thr Tyr Leu Glu Asn
770 775<210>375<211>941<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(918)<223>FRXA02100<400>375att gct ggt aag gca cac cca cat gac atg ggt ggc aag aag ctc atg 48Ile Ala Gly Lys Ala His Pro His Asp Met Gly Gly Lys Lys Leu Met1 5 10 15cag gaa atc gtc cac ttc gct gat caa gct ggt gtc cgt gac cgt ttc 96Gln Glu Ile Val His Phe Ala Asp Gln Ala Gly Val Arg Asp Arg Phe
20 25 30crc ttc ctg cct gat tac gac arc aac ctg gcc agc tac ctg atc tct 144Leu Phe Leu Pro Asp Tyr Asp Ile Asn Leu Ala Ser Tyr Leu Ile Ser
35 40 45ggt gct gac gtg tgg ctg aac aac cca gtg cgc cct cag gaa gca tcg 192Gly Ala Asp Val Trp Leu Asn Asn Pro Val Arg Pro Gln Glu Ala Ser
50 55 60gga acc tcc ggt atg aag gcc gtc atg aat ggt ggc ctg acc ctg tcc 240Gly Thr Ser Gly Met Lys Ala Val Met Asn Gly Gly Leu Thr Leu Ser65 70 75 80atc tct gat ggt tgg tgg gat gaa atg cct aag gag acc acc ggc tgg 288Ile Ser Asp Gly Trp Trp Asp Glu Met Pro Lys Glu Thr Thr Gly Trp
85 90 95acc atc cca acc gtt gag tcc cag gac ttg gaa tgc cgc gac cac ctg 336Thr Ile Pro Thr Val Glu Ser Gln Asp Leu Glu Cys Arg Asp His Leu
100 105 110gaa tcc cag gcg ctg tac gac ctg ctg gaa aac gaa gtt gca ccg ctg 384Glu Ser Gln Ala Leu Tyr Asp Leu Leu Glu Asn Glu Val Ala Pro Leu
115 120 125ttt tac aag cgc gac aag aac ggc atc cca cag gac tgg ctg gac ctg 432Phe Tyr Lys Arg Asp Lys Asn Gly Ile Pro Gln Asp Trp Leu Asp Leu
130 135 140gtt cgc gaa tcc tgg acc acc ctg tca cca atg gtc acc tcc acc cgc 480Val Arg Glu Ser Trp Thr Thr Leu Ser Pro Met Val Thr Ser Thr Arg145 150 155 160atg gtg cgc gac tac acc acc cag tac tac cgc cca acc aaa cac cag 528Met Val Arg Asp Tyr Thr Thr Gln Tyr Tyr Arg Pro Thr Lys His Gln
165 170 175gca gag ctc att gcg cag cct gca gaa gca gcg gat tac gcg gca tgg 576Ala Glu Leu Ile Ala Gln Pro Ala Glu Ala Ala Asp Tyr Ala Ala Trp
180 185 190ctt gag cac atc aaa gca gag tgg gct ggc gtc aag gtc tca gac ctg 624Leu Glu His Ile Lys Ala Glu Trp Ala Gly Val Lys Val Ser Asp Leu
195 200 205aag atc agc gag agc gcc atc acg gcg cag gag ctt gaa gtc agc gtt 672Lys Ile Ser Glu Ser Ala Ile Thr Ala Gln Glu Leu Glu Val Ser Val
210 215 220cgc gtt gat tcc ggt tcg ctt aac gac gac gag ttc caa gct cag gca 720Arg Val Asp Ser Gly Ser Leu Asn Asp Asp Glu Phe Gln Ala Gln Ala225 230 235 240ctc ttt ggt gcg ctc gga cac aac ggt gac atc gaa gat cca gaa atc 768Leu Phe Gly Ala Leu Gly His Asn Gly Asp Ile Glu Asp Pro Glu Ile
245 250 255acc gtt ttg acc cca cgc ggc gat ggc gcc tac gcg gca aag gtc agc 816Thr Val Leu Thr Pro Arg Gly Asp Gly Ala Tyr Ala Ala Lys Val Ser
260 265 270act gac ctg cca ggc aac tac ggc atc act gcc cgc gtt gtt cca aac 864Thr Asp Leu Pro Gly Asn Tyr Gly Ile Thr Ala Arg Val Val Pro Asn
275 280 285aac agg atg ctg gtc agc cca gcg gaa acc cgc ctg atc acc tac ttg 912Asn Arg Met Leu Val Ser Pro Ala Glu Thr Arg Leu Ile Thr Tyr Leu
290 295 300gag aac tagggcgaaa ctagctttac caa 941Glu Asn305<210>376<211>306<212>PRT<213>谷氨酸棒杆菌<400>376Ile Ala Gly Lys Ala His Pro His Asp Met Gly Gly Lys Lys Leu Met1 5 10 15Gln Glu Ile Val His Phe Ala Asp Gln Ala Gly Val Arg Asp Arg Phe
20 25 30Leu Phe Leu Pro Asp Tyr Asp Ile Asn Leu Ala Ser Tyr Leu Ile Ser
35 40 45Gly Ala Asp Val Trp Leu Asn Asn Pro Val Arg Pro Gln Glu Ala Ser
50 55 60Gly Thr Ser Gly Met Lys Ala Val Met Asn Gly Gly Leu Thr Leu Ser65 70 75 80Ile Ser Asp Gly Trp Trp Asp Glu Met Pro Lys Glu Thr Thr Gly Trp
85 90 95Thr Ile Pro Thr Val Glu Ser Gln Asp Leu Glu Cys Arg Asp His Leu
100 105 110Glu Ser Gln Ala Leu Tyr Asp Leu Leu Glu Asn Glu Val Ala Pro Leu
115 120 125Phe Tyr Lys Arg Asp Lys Asn Gly Ile Pro Gln Asp Trp Leu Asp Leu
130 135 140Val Arg Glu Ser Trp Thr Thr Leu Ser Pro Met Val Thr Ser Thr Arg145 150 155 160Met Val Arg Asp Tyr Thr Thr Gln Tyr Tyr Arg Pro Thr Lys His Gln
165 170 175Ala Glu Leu Ile Ala Gln Pro Ala Glu Ala Ala Asp Tyr Ala Ala Trp
180 185 190Leu Glu His Ile Lys Ala Glu Trp Ala Gly Val Lys Val Ser Asp Leu
195 200 205Lys Ile Ser Glu Ser Ala Ile Thr Ala Gln Glu Leu Glu Val Ser Val
210 215 220Arg Val Asp Ser Gly Ser Leu Asn Asp Asp Glu Phe Gln Ala Gln Ala225 230 235 240Leu Phe Gly Ala Leu Gly His Asn Gly Asp Ile Glu Asp Pro Glu Ile
245 250 255Thr Val Leu Thr Pro Arg Gly Asp Gly Ala Tyr Ala Ala Lys Val Ser
260 265 270Thr Asp Leu Pro Gly Asn Tyr Gly Ile Thr Ala Arg Val Val Pro Asn
275 280 285Asn Arg Met Leu Val Ser Pro Ala Glu Thr Arg Leu Ile Thr Tyr Leu
290 295 300Glu Asn305<210>377<211>1206<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1206)<223>FRXA02113<400>377cta ggt cga atc aac gcc gag gag caa aac ctc agc gaa tac ctc agc 48Leu Gly Arg Ile Asn Ala Glu Glu Gln Asn Leu Ser Glu Tyr Leu Ser1 5 10 15gac aag ctg tgg tac cag gac acc gca gat gca acc gat gct gtc gga 96Asp Lys Leu Trp Tyr Gln Asp Thr Ala Asp Ala Thr Asp Ala Val Gly
20 25 30gat cca ctc gtt gcg tac ttc tcc atg gag ttt ggc att cac cca agc 144Asp Pro Leu Val Ala Tyr Phe Ser Met Glu Phe Gly Ile His Pro Ser
35 40 45ctg cca atc tac tct ggc gga ctt ggt gtg ctt gcg ggc gag aac atg 192Leu Pro Ile Tyr Ser Gly Gly Leu Gly Val Leu Ala Gly Glu Asn Met
50 55 60aag tct gca tct gac ttg ggt gtg cea ctg atc ggt gtt ggt ttg ctc 240Lys Ser Ala Ser Asp Leu Gly Val Pro Leu Ile Gly Val Gly Leu Leu65 70 75 80tac acc cac ggc tac ttc acc cag tca ctg tcc ggt gac ggt tgg cag 288Tyr Thr His Gly Tyr Phe Thr Gln Ser Leu Ser Gly Asp Gly Trp Gln
85 90 95cag gaa gag tac aag tac cac gat cca gca gaa ctg ccg att gag gca 336Gln Glu Glu Tyr Lys Tyr His Asp Pro Ala Glu Leu Pro Ile Glu Ala
100 105 110gtt aaa gat aag aac ggc gag cag gtc act gtt tct gtc acc tac cca 384Val Lys Asp Lys Asn Gly Glu Gln Val Thr Val Ser Val Thr Tyr Pro
115 120 125ggt gcg cag gaa gta aag att gca ctg tgg gta gca aac gtt ggc cgc 432Gly Ala Gln Glu Val Lys Ile Ala Leu Trp Val Ala Asn Val Gly Arg
130 135 140atc cca ttg ctg ctg ctt gat acc aac atc gag gca aac cea gaa gag 480Ile Pro Leu Leu Leu Leu Asp Thr Asn Ile Glu Ala Asn Pro Glu Glu145 150 155 160ctc cgc aac gtt act gac cgc ctg tac ggt ggc gac aat gag cac cgc 528Leu Arg Asn Val Thr Asp Arg Leu Tyr Gly Gly Asp Asn Glu His Arg
165 170 175atc aag cag gaa ctc gtt ctc ggt gtt ggt ggc gtc cgc gct gtc aac 576Ile Lys Gln Glu Leu Val Leu Gly Val Gly Gly Val Arg Ala Val Asn
180 185 190gca ttc tgc gaa gct cgt ggt ctg aag cgc tca tct gtt gca cac ctc 624Ala Phe Cys Glu Ala Arg Gly Leu Lys Arg Ser Ser Val Ala His Leu
195 200 205aac gaa ggc cac gca ggt ttc ctg acc ctg gag cgt atc cgc gag cgc 672Asn Glu Gly His Ala Gly Phe Leu Thr Leu Glu Arg Ile Arg Glu Arg
210 215 220atc gca gag ggc atg gag tac cca gca gca ttc gag cag gtt cgt gcg 720Ile Ala Glu Gly Met Glu Tyr Pro Ala Ala Phe Glu Gln Val Arg Ala225 230 235 240tcc aac atc ttc acc acc cac acc cca gtc cca gca ggc atc gac cgc 768Ser Asn Ile Phe Thr Thr His Thr Pro Val Pro Ala Gly Ile Asp Arg
245 250 255ttc gac atg gag atg gtg cgt cgt tat ctc ggt ggc ggt cag cca gaa 816Phe Asp Met Glu Met Val Arg Arg Tyr Leu Gly Gly Gly Gln Pro Glu
260 265 270gat cag cag ctg tgc gtt ggt gtt cca att gag aag gca ctt gag ctt 864Asp Gln Gln Leu Cys Val Gly Val Pro Ile Glu Lys Ala Leu Glu Leu
275 280 285ggt caa gag tcc gat cca cac cgc ttc aac atg gct cat atg ggc ctt 912Gly Gln Glu Ser Asp Pro His Arg Phe Asn Met Ala His Met Gly Leu
290 295 300cgc gcg agc caa cat gct aat ggc gtc gca aag ctt cat ggt gaa gta 960Arg Ala Ser Gln His Ala Asn Gly Val Ala Lys Leu His Gly Glu Val305 310 315 320agc cgt gac atg ttc gcc ggc ctg tac ccc gga tat gag cct cgt gaa 1008Ser Arg Asp Met Phe Ala Gly Leu Tyr Pro Gly Tyr Glu Pro Arg Glu
325 330 335gtg ccc atc ggg cac gtc acc aac ggt gtt cac ctg ccg acg tgg gtc 1056Val Pro Ile Gly His Val Thr Asn Gly Val His Leu Pro Thr Trp Val
340 345 350aag cca gag atg aag gaa ctc atc gat cgc gtc act ggc ggc gct gat 1104Lys Pro Glu Met Lys Glu Leu Ile Asp Arg Val Thr Gly Gly Ala Asp
355 360 365ctt gcg gtt gct gat tct tgg tca aac cca cag gct gtc gag tct gag 1152Leu Ala Val Ala Asp Ser Trp Ser Asn Pro Gln Ala Val Glu Ser Glu
370 375 380aag atc tgg aag gtg cgc aac aag ttc cgt gct gac cta gtg gag gtt 1200Lys Ile Trp Lys Val Arg Asn Lys Phe Arg Ala Asp Leu Val Glu Val385 390 395 400gct cgc 1206Ala Arg<210>378<211>402<212>PRT<213>谷氨酸棒杆菌<400>378Leu Gly Arg Ile Asn Ala Glu Glu Gln Asn Leu Ser Glu Tyr Leu Ser1 5 10 15Asp Lys Leu Trp Tyr Gln Asp Thr Ala Asp Ala Thr Asp Ala Val Gly
20 25 30Asp Pro Leu Val Ala Tyr Phe Ser Met Glu Phe Gly Ile His Pro Ser
35 40 45Leu Pro Ile Tyr Ser Gly Gly Leu Gly Val Leu Ala Gly Glu Asn Met
50 55 60Lys Ser Ala Ser Asp Leu Gly Val Pro Leu Ile Gly Val Gly Leu Leu65 70 75 80Tyr Thr His Gly Tyr Phe Thr Gln Ser Leu Ser Gly Asp Gly Trp Gln
85 90 95Gln Glu Glu Tyr Lys Tyr His Asp Pro Ala Glu Leu Pro Ile Glu Ala
100 105 110Val Lys Asp Lys Asn Gly Glu Gln Val Thr Val Ser Val Thr Tyr Pro
115 120 125Gly Ala Gln Glu Val Lys Ile Ala Leu Trp Val Ala Asn Val Gly Arg
130 135 140Ile Pro Leu Leu Leu Leu Asp Thr Asn Ile Glu Ala Asn Pro Glu Glu145 150 155 160Leu Arg Asn Val Thr Asp Arg Leu Tyr Gly Gly Asp Asn Glu His Arg
165 170 175Ile Lys Gln Glu Leu Val Leu Gly Val Gly Gly Val Arg Ala Val Asn
180 185 190Ala Phe Cys Glu Ala Arg Gly Leu Lys Arg Ser Ser Val Ala His Leu
195 200 205Asn Glu Gly His Ala Gly Phe Leu Thr Leu Glu Arg Ile Arg Glu Arg
210 215 220Ile Ala Glu Gly Met Glu Tyr Pro Ala Ala Phe Glu Gln Val Arg Ala225 230 235 240Ser Asn Ile Phe Thr Thr His Thr Pro Val Pro Ala Gly Ile Asp Arg
245 250 255Phe Asp Met Glu Met Val Arg Arg Tyr Leu Gly Gly Gly Gln Pro Glu
260 265 270Asp Gln Gln Leu Cys Val Gly Val Pro Ile Glu Lys Ala Leu Glu Leu
275 280 285Gly Gln Glu Ser Asp Pro His Arg Phe Asn Met Ala His Met Gly Leu
290 295 300Arg Ala Ser Gln His Ala Asn Gly Val Ala Lys Leu His Gly Glu Val305 310 315 320Ser Arg Asp Met Phe Ala Gly Leu Tyr Pro Gly Tyr Glu Pro Arg Glu
325 330 335Val Pro Ile Gly His Val Thr Asn Gly Val His Leu Pro Thr Trp Val
340 345 350Lys Pro Glu Met Lys Glu Leu Ile Asp Arg Val Thr Gly Gly Ala Asp
355 360 365Leu Ala Val Ala Asp Ser Trp Ser Asn Pro Gln Ala Val Glu Ser Glu
370 375 380Lys Ile Trp Lys Val Arg Asn Lys Phe Arg Ala Asp Leu Val Glu Val385 390 395 400Ala Arg<210>379<211>1140<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1117)<223>RXA02147<400>379aaaagttgag agcggcttgc ttcttttcct tggctaggct tttgtaatcg ggttagagta 60gtggagttgc ttgaatgagg ttgatagggg atttttgaag atg ttt ggt cgc cgt 115
Met Phe Gly Arg Arg
1 5tgg gtg agc gtt gtg gcg tca tgt gtt atc gca agc acg ctg att ctg 163Trp Val Ser Val Val Ala Ser Cys Val Ile Ala Ser Thr Leu Ile Leu
10 15 20gtg cct tcg cat tcc ggt gcg gag gaa gtc gat caa ctg att gct gat 211Val Pro Ser His Ser Gly Ala Glu Glu Val Asp Gln Leu Ile Ala Asp
25 30 35atc gag cat gtc tct cag gaa acg tct gcc cag aat gag gaa gtc aaa 259Ile Glu His Val Ser Gln Glu Thr Ser Ala Gln Asn Glu Glu Val Lys
40 45 50cag ctt gag att gat att gag gct cgt gag gtc acg atc aag gaa gtt 307Gln Leu Glu Ile Asp Ile Glu Ala Arg Glu Val Thr Ile Lys Glu Val
55 60 65cag gag cag tcg gta agc tac cgt gag gcg gct gat caa gca tcg gag 355Gln Glu Gln Ser Val Ser Tyr Arg Glu Ala Ala Asp Gln Ala Ser Glu70 75 80 85aat gtc gaa gct tat cgt tcg gag atc aat cgg atc gct cag gcg aag 403Asn Val Glu Ala Tyr Arg Ser Glu Ile Asn Arg Ile Ala Gln Ala Lys
90 95 100tat cgt ggc aca gtc acg gat cct ttg agc att gcg gtg tct gca gaa 451Tyr Arg Gly Thr Val Thr Asp Pro Leu Ser Ile Ala Val Ser Ala Glu
105 110 115gat cca caa aac gtg att gat cgg atg agc tac ctt tca acg ttg act 499Asp Pro Gln Asn Val Ile Asp Arg Met Ser Tyr Leu Ser Thr Leu Thr
120 125 130aag tcc act agt gat gtg gtt gaa tcc ctc aac gcg gag act gag aag 547Lys Ser Thr Ser Asp Val Val Glu Ser Leu Asn Ala Glu Thr Glu Lys
135 140 145tcc gca gaa gct gtg tat caa gca aac cgt act aag gcg gaa gcg gag 595Ser Ala Glu Ala Val Tyr Gln Ala Asn Arg Thr Lys Ala Glu Ala Glu150 155 160 165ttc cag ttg ggg cag ctg aag gta cgc cag gcg gag ctt gaa tct gaa 643Phe Gln Leu Gly Gln Leu Lys Val Arg Gln Ala Glu Leu Glu Ser Glu
170 175 180aag gaa gca ttg gat ggt cga aaa tcg gag atc cga gac cgg gtg gat 691Lys Glu Ala Leu Asp Gly Arg Lys Ser Glu Ile Arg Asp Arg Val Asp
185 190 195gcc ctg acg cca cag gag cgg gaa atg tgg gtt gct aag aat ggt cca 739Ala Leu Thr Pro Gln Glu Arg Glu Met Trp Val Ala Lys Asn Gly Pro
200 205 210ttg gac att gat ctg act gat ttg ctt ggt ctt tcc gct gcg act tcg 787Leu Asp Ile Asp Leu Thr Asp Leu Leu Gly Leu Ser Ala Ala Thr Ser
215 220 225ggt gcg gtg gat gct gcc ttg tct aag ttg gga agc cct tat ggt tgg 835Gly Ala Val Asp Ala Ala Leu Ser Lys Leu Gly Ser Pro Tyr Gly Trp230 235 240 245ggt ggc att ggc cca aat gag ttt gat tgc tca ggt ttg atc tat tgg 883Gly Gly Ile Gly Pro Asn Glu Phe Asp Cys Ser Gly Leu Ile Tyr Trp
250 255 260gcg tat cag cag atg ggt aag act ttg cca cgt acg tct caa gct cag 931Ala Tyr Gln Gln Met Gly Lys Thr Leu Pro Arg Thr Ser Gln Ala Gln
265 270 275atg gct ggc gga acg ccg gtg agc aga gat gag ctg cag cct ggc gat 979Met Ala Gly Gly Thr Pro Val Ser Arg Asp Glu Leu Gln Pro Gly Asp
280 285 290gtc att gga tat tac cca ggt gct act cac gtg gga ctg tat att ggg 1027Val Ile Gly Tyr Tyr Pro Gly Ala Thr His Val Gly Leu Tyr Ile Gly
295 300 305gac gga aag att gtg cac gcc tca gac tac gga atc cct gtg cag gtg 1075Asp Gly Lys Ile Val His Ala Ser Asp Tyr Gly Ile Pro Val Gln Val310 315 320 325gta tct gtt gat tca gca ccg ttt tat ggt gcg cgt cgc tac 1117Val Ser Val Asp Ser Ala Pro Phe Tyr Gly Ala Arg Arg Tyr
330 335taagaaatag ttcgtcagga gaa 1140<210>380<211>339<212>PRT<213>谷氨酸棒杆菌<400>380Met Phe Gly Arg Arg Trp Val Ser Val Val Ala Ser Cys Val Ile Ala1 5 10 15Ser Thr Leu Ile Leu Val Pro Ser His Ser Gly Ala Glu Glu Val Asp
20 25 30Gln Leu Ile Ala Asp Ile Glu His Val Ser Gln Glu Thr Ser Ala Gln
35 40 45Asn Glu Glu Val Lys Gln Leu Glu Ile Asp Ile Glu Ala Arg Glu Val
50 55 60Thr Ile Lys Glu Val Gln Glu Gln Ser Val Ser Tyr Arg Glu Ala Ala65 70 75 80Asp Gln Ala Ser Glu Asn Val Glu Ala Tyr Arg Ser Glu Ile Asn Arg
85 90 95Ile Ala Gln Ala Lys Tyr Arg Gly Thr Val Thr Asp Pro Leu Ser Ile
100 105 110Ala Val Ser Ala Glu Asp Pro Gln Asn Val Ile Asp Arg Met Ser Tyr
115 120 125Leu Ser Thr Leu Thr Lys Ser Thr Ser Asp Val Val Glu Ser Leu Asn
130 135 140Ala Glu Thr Glu Lys Ser Ala Glu Ala Val Tyr Gln Ala Asn Arg Thr145 150 155 160Lys Ala Glu Ala Glu Phe Gln Leu Gly Gln Leu Lys Val Arg Gln Ala
165 170 175Glu Leu Glu Ser Glu Lys Glu Ala Leu Asp Gly Arg Lys Ser Glu Ile
180 185 190Arg Asp Arg Val Asp Ala Leu Thr Pro Gln Glu Arg Glu Met Trp Val
195 200 205Ala Lys Asn Gly Pro Leu Asp Ile Asp Leu Thr Asp Leu Leu Gly Leu
210 215 220Ser Ala Ala Thr Ser Gly Ala Val Asp Ala Ala Leu Ser Lys Leu Gly225 230 235 240Ser Pro Tyr Gly Trp Gly Gly Ile Gly Pro Asn Glu Phe Asp Cys Ser
245 250 255Gly Leu Ile Tyr Trp Ala Tyr Gln Gln Met Gly Lys Thr Leu Pro Arg
260 265 270Thr Ser Gln Ala Gln Met Ala Gly Gly Thr Pro Val Ser Arg Asp Glu
275 280 285Leu Gln Pro Gly Asp Val Ile Gly Tyr Tyr Pro Gly Ala Thr His Val
290 295 300Gly Leu Tyr Ile Gly Asp Gly Lys Ile Val His Ala Ser Asp Tyr Gly305 310 315 320Ile Pro Val Gln Val Val Ser Val Asp Ser Ala Pro Phe Tyr Gly Ala
325 330 335Arg Arg Tyr<2l0>381<211>1959<212>DNA<213>谷氨酸棒杆菌<220><22l>CDS<222>(101)..(1936)<223>RXA01478<400>381gcgggttttg ttgtggaggg gcgcgtcgaa aagcaatttt ttttccaaga tagctcactt 60tattggagtc acctggcctg aaatcctcta ctctgggcgc atg acc att cca gga 115
Met Thr Ile Pro Gly
1 5gct tcc aca cag act gat atc cct ctg gac aca ctt ctt gag gat tac 163Ala Ser Thr Gln Thr Asp Ile Pro Leu Asp Thr Leu Leu Glu Asp Tyr
10 15 20gcg cta ctg tca gac act cac acc ggc gct ctg ctg tcc aac atg ggc 211Ala Leu Leu Ser Asp Thr His Thr Gly Ala Leu Leu Ser Asn Met Gly
25 30 35agt ttg gac tgg ttg tgc ctg cct cgt ttt gat tcc caa gcc atg ttc 259Ser Leu Asp Trp Leu Cys Leu Pro Arg Phe Asp Ser Gln Ala Met Phe
40 45 50acc agg ctg ctt ggt gat cgc gag cac gga cac tgg agt ttg cgt gtc 307Thr Arg Leu Leu Gly Asp Arg Glu His Gly His Trp Ser Leu Arg Val
55 60 65cca ggt ggt gag gtg atc agc caa aac tac ctc ggc gat tcc ttc gtg 355Pro Gly Gly Glu Val Ile Ser Gln Asn Tyr Leu Gly Asp Ser Phe Val70 75 80 85gtg cag acc gtg tgg cgt tca gag acc ggt act gcc cgg gtt gtt gat 403Val Gln Thr Val Trp Arg Ser Glu Thr Gly Thr Ala Arg Val Val Asp
90 95 100ttc atg cca att cac ggt caa gaa caa ccc gat atc acc gac ctg gtg 451Phe Met Pro Ile His Gly Gln Glu Gln Pro Asp Ile Thr Asp Leu Val
105 110 115cgc tct gtg cac tgc gtg gaa ggc gaa gtg gat gtg gaa tcg atc ctg 499Arg Ser Val His Cys Val Glu Gly Glu Val Asp Val Glu Ser Ile Leu
120 125 130cgc ctg cgt ttt gat tat ggc gag tcc act ccg tat ttc cgc acc agc 547Arg Leu Arg Phe Asp Tyr Gly Glu Ser Thr Pro Tyr Phe Arg Thr Ser
135 140 145act gtc gac ggc atc agc atc gtg cag gct gtc gcc ggc ccc aat gcg 595Thr Val Asp Gly Ile Ser Ile Val Gln Ala Val Ala Gly Pro Asn Ala150 155 160 165gta tat gtt cgt gga cct gag atg cca cac cgc cct gca aag gat tgt 643Val Tyr Val Arg Gly Pro Glu Met Pro His Arg Pro Ala Lys Asp Cys
170 175 180cac agt ggc acc ttc cac ctg acg gcc ggc gaa tcc gtg gaa tgg gtt 691His Ser Gly Thr Phe His Leu Thr Ala Gly Glu Ser Val Glu Trp Val
185 190 195ctc acc tgg gca ccg tcg ttc gaa ccg cat ccc ccc atg ccg gat tac 739Leu Thr Trp Ala Pro Ser Phe Glu Pro His Pro Pro Met Pro Asp Tyr
200 205 210acc cgc tct ttg gag agc acc ttg agc ttc tgg gca tca tgg gtt gaa 787Thr Arg Ser Leu Glu Ser Thr Leu Ser Phe Trp Ala Ser Trp Val Glu
215 220 225gag ctc ccc cac cag cgc ctc tac gac gct gaa gtc cgc cgc tcc atg 835Glu Leu Pro His Gln Arg Leu Tyr Asp Ala Glu Val Arg Arg Ser Met230 235 240 245ctc gta ctg cgc gcc ttg acc gat cta caa acc ggt ggc atc gtg gcc 883Leu Val Leu Arg Ala Leu Thr Asp Leu Gln Thr Gly Gly Ile Val Ala
250 255 260gca ccg acc acc tca cta cca gag gat ttc gga ggc atc cgt aac tgg 931Ala Pro Thr Thr Ser Leu Pro Glu Asp Phe Gly Gly Ile Arg Asn Trp
265 270 275gac tac cgc tac gtg tgg ctg cgc gac tcc gca ctc acc att gaa gcc 979Asp Tyr Arg Tyr Val Trp Leu Arg Asp Ser Ala Leu Thr Ile Glu Ala
280 285 290ctc gtg gaa tac gga ttc tcc caa gca gcc ctc caa tgg cgc acc tgg 1027Leu Val Glu Tyr Gly Phe Ser Gln Ala Ala Leu Gln Trp Arg Thr Trp
295 300 305ctg ctg cgc gcc atc gca ggc gac ccg gaa aac ctc cgc atc atg tat 1075Leu Leu Arg Ala Ile Ala Gly Asp Pro Glu Asn Leu Arg Ile Met Tyr310 315 320 325ggc ctc ggc ggc gaa cga cac ctc cct gaa cgc gaa ctc caa cac ctg 1123Gly Leu Gly Gly Glu Arg His Leu Pro Glu Arg Glu Leu Gln His Leu
330 335 340cgc gga tac gaa aac tcc gtg cct gtt cgc gtt ggc aat gga gcc gcc 1171Arg Gly Tyr Glu Asn Ser Val Pro Val Arg Val Gly Asn Gly Ala Ala
345 350 355gaa caa tac caa gca gat gtc gtc ggc gaa gta atg gtc gcg ctt gaa 1219Glu Gln Tyr Gln Ala Asp Val Val Gly Glu Val Met Val Ala Leu Glu
360 365 370acc atc cgc cgc gcc ggg tgc ctc gag gac gaa ttc tcc tgg ggc atg 1267Thr Ile Arg Arg Ala Gly Cys Leu Glu Asp Glu Phe Ser Trp Gly Met
375 380 385caa aaa gcc atc ctc gat ttc caa gaa gcc aac ttc gac cgc aag gat 1315Gln Lys Ala Ile Leu Asp Phe Gln Glu Ala Asn Phe Asp Arg Lys Asp390 395 400 405caa ggc atc tgg gaa atg cgc tcc gaa ccg caa tat ttc acc cac ggc 1363Gln Gly Ile Trp Glu Met Arg Ser Glu Pro Gln Tyr Phe Thr His Gly
410 415 420cgc gcc atg atg tgg gcc ggc ttc gac cgc ggc atc aaa gcc atc gaa 1411Arg Ala Met Met Trp Ala Gly Phe Asp Arg Gly Ile Lys Ala Ile Glu
425 430 435gaa ttc aac ctc gac ggc ccc atc gag cgc tgg cgt gaa ctc cgc gcc 1459Glu Phe Asn Leu Asp Gly Pro Ile Glu Arg Trp Arg Glu Leu Arg Ala
440 445 450aaa ctc cgc gaa gaa atc atg acc aac ggc ttc aac gaa gag atc caa 1507Lys Leu Arg Glu Glu Ile Met Thr Asn Gly Phe Asn Glu Glu Ile Gln
455 460 465tcc ttc acc cag tgc tac gac aac acc caa gtc gac gcc tcg ctg ctt 1555Ser Phe Thr Gln Cys Tyr Asp Asn Thr Gln Val Asp Ala Ser Leu Leu470 475 480 485cag ctc gcc caa ata ggc ttc atc ggc ttc gac gat cca aaa atg ctc 1603Gln Leu Ala Gln Ile Gly Phe Ile Gly Phe Asp Asp Pro Lys Met Leu
490 495 500agc acc gta gcg cgc att gag caa gag ctt ctc gac gcc cac ggc ttt 1651Ser Thr Val Ala Arg Ile Glu Gln Glu Leu Leu Asp Ala His Gly Phe
505 510 515ctt cac agg tac cac acc gac ggg tct gac ggc ctt gcc ggc gac gaa 1699Leu His Arg Tyr His Thr Asp Gly Ser Asp Gly Leu Ala Gly Asp Glu
520 525 530tac ccc ttc ctc atc tgt tca ttc tgg ctg gta gaa caa tac gca agc 1747Tyr Pro Phe Leu Ile Cys Ser Phe Trp Leu Val Glu Gln Tyr Ala Ser
535 540 545tcc aac cgc ctc gac gag gcc aaa gaa aag atg aac cgc atc ctt gcc 1795Ser Asn Arg Leu Asp Glu Ala Lys Glu Lys Met Asn Arg Ile Leu Ala550 555 560 565gtc caa agc cca ctt ggc cta ctg gct gag gaa tac tcc acc cac cat 1843Val Gln Ser Pro Leu Gly Leu Leu Ala Glu Glu Tyr Ser Thr His His
570 575 580ggc agg ctc gct gga aac tac cct cag gcc ttt tcc cac att ggt ctg 1891Gly Arg Leu Ala Gly Asn Tyr Pro Gln Ala Phe Ser His Ile Gly Leu
585 590 595atc agc gct gcc cgt gcc ata aat ttc gaa gaa gcg cga aac agg 1936Ile Ser Ala Ala Arg Ala Ile Asn Phe Glu Glu Ala Arg Asn Arg
600 605 610tagagtctaa ggtgtcattc ttg 1959<210>382<211>612<212>PRT<213>谷氨酸棒杆菌<400>382Met Thr Ile Pro Gly Ala Ser Thr Gln Thr Asp Ile Pro Leu Asp Thr1 5 10 15Leu Leu Glu Asp Tyr Ala Leu Leu Ser Asp Thr His Thr Gly Ala Leu
20 25 30Leu Ser Asn Met Gly Ser Leu Asp Trp Leu Cys Leu Pro Arg Phe Asp
35 40 45Ser Gln Ala Met Phe Thr Arg Leu Leu Gly Asp Arg Glu His Gly His
50 55 60Trp Ser Leu Arg Val Pro Gly Gly Glu Val Ile Ser Gln Asn Tyr Leu65 70 75 80Gly Asp Ser Phe Val Val Gln Thr Val Trp Arg Ser Glu Thr Gly Thr
85 90 95Ala Arg Val Val Asp Phe Met Pro Ile His Gly Gln Glu Gln Pro Asp
100 105 110Ile Thr Asp Leu Val Arg Ser Val His Cys Val Glu Gly Glu Val Asp
115 120 125Val Glu Ser Ile Leu Arg Leu Arg Phe Asp Tyr Gly Glu Ser Thr Pro
130 135 140Tyr Phe Arg Thr Ser Thr Val Asp Gly Ile Ser Ile Val Gln Ala Val145 150 155 160Ala Gly Pro Asn Ala Val Tyr Val Arg Gly Pro Glu Met Pro His Arg
165 170 175Pro Ala Lys Asp Cys His Ser Gly Thr Phe His Leu Thr Ala Gly Glu
180 185 190Ser Val Glu Trp Val Leu Thr Trp Ala Pro Ser Phe Glu Pro His Pro
195 200 205Pro Met Pro Asp Tyr Thr Arg Ser Leu Glu Ser Thr Leu Ser Phe Trp
210 215 220Ala Ser Trp Val Glu Glu Leu Pro His Gln Arg Leu Tyr Asp Ala Glu225 230 235 240Val Arg Arg Ser Met Leu Val Leu Arg Ala Leu Thr Asp Leu Gln Thr
245 250 255Gly Gly Ile Val Ala Ala Pro Thr Thr Ser Leu Pro Glu Asp Phe Gly
260 265 270Gly Ile Arg Asn Trp Asp Tyr Arg Tyr Val Trp Leu Arg Asp Ser Ala
275 280 285Leu Thr Ile Glu Ala Leu Val Glu Tyr Gly Phe Ser Gln Ala Ala Leu
290 295 300Gln Trp Arg Thr Trp Leu Leu Arg Ala Ile Ala Gly Asp Pro Glu Asn305 310 315 320Leu Arg Ile Met Tyr Gly Leu Gly Gly Glu Arg His Leu Pro Glu Arg
325 330 335Glu Leu Gln His Leu Arg Gly Tyr Glu Asn Ser Val Pro Val Arg Val
340 345 350Gly Asn Gly Ala Ala Glu Gln Tyr Gln Ala Asp Val Val Gly Glu Val
355 360 365Met Val Ala Leu Glu Thr Ile Arg Arg Ala Gly Cys Leu Glu Asp Glu
370 375 380Phe Ser Trp Gly Met Gln Lys Ala Ile Leu Asp Phe Gln Glu Ala Asn385 390 395 400Phe Asp Arg Lys Asp Gln Gly Ile Trp Glu Met Arg Ser Glu Pro Gln
405 410 415Tyr Phe Thr His Gly Arg Ala Met Met Trp Ala Gly Phe Asp Arg Gly
420 425 430Ile Lys Ala Ile Glu Glu Phe Asn Leu Asp Gly Pro Ile Glu Arg Trp
435 440 445Arg Glu Leu Arg Ala Lys Leu Arg Glu Glu Ile Met Thr Asn Gly Phe
450 455 460Asn Glu Glu Ile Gln Ser Phe Thr Gln Cys Tyr Asp Asn Thr Gln Val465 470 475 480Asp Ala Ser Leu Leu Gln Leu Ala Gln Ile Gly Phe Ile Gly Phe Asp
485 490 495Asp Pro Lys Met Leu Ser Thr Val Ala Arg Ile Glu Gln Glu Leu Leu
500 505 510Asp Ala His Gly Phe Leu His Arg Tyr His Thr Asp Gly Ser Asp Gly
515 520 525Leu Ala Gly Asp Glu Tyr Pro Phe Leu Ile Cys Ser Phe Trp Leu Val
530 535 540Glu Gln Tyr Ala Ser Ser Asn Arg Leu Asp Glu Ala Lys Glu Lys Met545 550 555 560Asn Arg Ile Leu Ala Val Gln Ser Pro Leu Gly Leu Leu Ala Glu Glu
565 570 575Tyr Ser Thr His His Gly Arg Leu Ala Gly Asn Tyr Pro Gln Ala Phe
580 585 590Ser His Ile Gly Leu Ile Ser Ala Ala Arg Ala Ile Asn Phe Glu Glu
595 600 605Ala Arg Asn Arg
610<210>383<211>658<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(658)<223>RXA01888<400>383agtagatact agataccacc cattgatgcc gtcaaggggt ttcctgtaaa gatgtaagag 60attaagaaaa gaggtagata tggcgtcaaa gcgaccgaca atg gct gat gtg gca 115
Met Ala Asp Val Ala
1 5aaa gct gct gga gta tcc act gcg ctg gtc tcc atc gtg ttt cgc gat 163Lys Ala Ala Gly Val Ser Thr Ala Leu Val Ser Ile Val Phe Arg Asp
10 15 20gcc ccc gga gca agt gaa tcc acc cgc aac cat gtg aaa gaa aaa gcc 211Ala Pro Gly Ala Ser Glu Ser Thr Arg Asn His Val Lys Glu Lys Ala
25 30 35gcc gaa ctc gga tac att cct gat cga cga gcc caa aaa ctt cgc caa 259Ala Glu Leu Gly Tyr Ile Pro Asp Arg Arg Ala Gln Lys Leu Arg Gln
40 45 50aac cgc tcc gga ctc atc ggt gtg gca ttc gaa atg cac caa gca ttc 307Asn Arg Ser Gly Leu Ile Gly Val Ala Phe Glu Met His Gln Ala Phe
55 60 65cac ggc gat atc gtc gaa cac ctc tat ccc acc gcc cga aaa cat ggc 355His Gly Asp Ile Val Glu His Leu Tyr Pro Thr Ala Arg Lys His Gly70 75 80 85ttc gac ctg tac ctt agc gcg atc aca ccg act cgc act gaa aaa gat 403Phe Asp Leu Tyr Leu Ser Ala Ile Thr Pro Thr Arg Thr Glu Lys Asp
90 95 100gcc gtc aac gcc ctg atc agg gaa cga tgc gaa gca gta att ctt cta 451Ala Val Asn Ala Leu Ile Arg Glu Arg Cys Glu Ala Val Ile Leu Leu
105 110 115gga tct cgc atg tca cct agt gat ttg gaa aca atc gca cag caa ctt 499Gly Ser Arg Met Ser Pro Ser Asp Leu Glu Thr Ile Ala Gln Gln Leu
120 125 130ccc gtc caa gta att gcc cgc ggt tcc gga acc ccc aaa gtc agt tcc 547Pro Val Gln Val Ile Ala Arg Gly Ser Gly Thr Pro Lys Val Ser Ser
135 140 145gtc cat gtc gac gac gca gtt ggc gcc caa tta gcc ctc aac cac ctc 595Val His Val Asp Asp Ala Val Gly Ala Gln Leu Ala Leu Asn His Leu150 155 160 165atc gaa tta ggc cac gaa cac atc atc tac atc gat ggt ggc gac gcc 643Ile Glu Leu Gly His Glu His Ile Ile Tyr Ile Asp Gly Gly Asp Ala
170 175 180cct ggc acc cag gaa 658Pro Gly Thr Gln Glu
185<210>384<211>186<212>PRT<213>谷氨酸棒杆菌<400>384Met Ala Asp Val Ala Lys Ala Ala Gly Val Ser Thr Ala Leu Val Ser1 5 10 15Ile Val Phe Arg Asp Ala Pro Gly Ala Ser Glu Ser Thr Arg Asn His
20 25 30Val Lys Glu Lys Ala Ala Glu Leu Gly Tyr Ile Pro Asp Arg Arg Ala
35 40 45Gln Lys Leu Arg Gln Asn Arg Ser Gly Leu Ile Gly Val Ala Phe Glu
50 55 60Met His Gln Ala Phe His Gly Asp Ile Val Glu His Leu Tyr Pro Thr65 70 75 80Ala Arg Lys His Gly Phe Asp Leu Tyr Leu Ser Ala Ile Thr Pro Thr
85 90 95Arg Thr Glu Lys Asp Ala Val Asn Ala Leu Ile Arg Glu Arg Cys Glu
100 105 110Ala Val Ile Leu Leu Gly Ser Arg Met Ser Pro Ser Asp Leu Glu Thr
115 120 125Ile Ala Gln Gln Leu Pro Val Gln Val Ile Ala Arg Gly Ser Gly Thr
130 135 140Pro Lys Val Ser Ser Val His Val Asp Asp Ala Val Gly Ala Gln Leu145 150 155 160Ala Leu Asn His Leu Ile Glu Leu Gly His Glu His Ile Ile Tyr Ile
165 170 175Asp Gly Gly Asp Ala Pro Gly Thr Gln Glu
180 185<210>385<211>1503<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1480)<223>RXN01927<400>385gagcagcggg atcttttgcg taattcgcgc gcgcagatcc atgtgattga ccacaatggt 60gatgaaattt tggatacccc aacggaagag gatttttaag atg gct ttg gtt ctt 115
Met Ala Leu Val Leu
1 5gga atc gat agt tcc acc caa tcc tgc aag gct ttg ctt gtc gac gcc 163Gly Ile Asp Ser Ser Thr Gln Ser Cys Lys Ala Leu Leu Val Asp Ala
10 15 20gcc acc ggc cag gtt atc gac gaa ggc cgc gcg agt cac ccg agc ggg 211Ala Thr Gly Gln Val Ile Asp Glu Gly Arg Ala Ser His Pro Ser Gly
25 30 35tcg gag gta gat cca cgt gcg tgg atc gct gcg ctg gat caa gct acc 259Ser Glu Val Asp Pro Arg Ala Trp Ile Ala Ala Leu Asp Gln Ala Thr
40 45 50gag ggg ttg tta gaa cgc gcg gac gct gta tct att gca ggc cag cag 307Glu Gly Leu Leu Glu Arg Ala Asp Ala Val Ser Ile Ala Gly Gln Gln
55 60 65cac ggc atg gtg gcg ttg gat gaa aac gat gaa atc gtt cgc ccg gcg 355His Gly Met Val Ala Leu Asp Glu Asn Asp Glu Ile Val Arg Pro Ala70 75 80 85ttg tta tgg aat gac act cgt tct gcc cag gct gcg ttg gat ctc aat 403Leu Leu Trp Asn Asp Thr Arg Ser Ala Gln Ala Ala Leu Asp Leu Asn
90 95 100gag gag atc ggc ggc gat cag gct gcg gta gat gcc acg gga agt gtg 451Glu Glu Ile Gly Gly Asp Gln Ala Ala Val Asp Ala Thr Gly Ser Val
105 110 115tat gtt gct tct tta act gcc acc aaa atg cgg tgg atg cgt gat cat 499Tyr Val Ala Ser Leu Thr Ala Thr Lys Met Arg Trp Met Arg Asp His
120 125 130gaa cca gaa aat gca gcg cgc acg gcg tcg gtg atg ttg cct cat gat 547Glu Pro Glu Asn Ala Ala Arg Thr Ala Ser Val Met Leu Pro His Asp
135 140 145ttc ctc acc tgg cat ttg atg gga cgc gga cgc aaa gtc acc gac cat 595Phe Leu Thr Trp His Leu Met Gly Arg Gly Arg Lys Val Thr Asp His150 155 160 165ggt gat gct tct gga acg ggc tac tac agc acg cgt gat cgt gcg tgg 643Gly Asp Ala Ser Gly Thr Gly Tyr Tyr Ser TAr Arg Asp Arg Ala Trp
170 175 180cgc acc gat cta gct gcc ttg gcg ctg ggc cat gag gtg gaa ctt cct 691Arg Thr Asp Leu Ala Ala Leu Ala Leu Gly His Glu Val Glu Leu Pro
185 190 195gaa ctc ctg gcc cca aat gcg att gcg gga aca act cca ggt gga gtg 739Glu Leu Leu Ala Pro Asn Ala Ile Ala Gly Thr Thr Pro Gly Gly Val
200 205 210aaa gtt gct gca ggc acg gga gat aat gct gcg gct gcg ctt ggc ctt 787Lys Val Ala Ala Gly Thr Gly Asp Asn Ala Ala Ala Ala Leu Gly Leu
215 220 225gat ttg cag cct ggt gat gtc agc gtg tcg atc ggc acc tct ggc gtt 835Asp Leu Gln Pro Gly Asp Val Ser Val Ser Ile Gly TAr Ser Gly Val230 235 240 245gcc ggc atg acc gtt caa cat agc gtc cac gat cca tct ggt ttg gtc 883Ala Gly Met Thr Val Gln His Ser Val His Asp Pro Ser Gly Leu Val
250 255 260act ggt ttc gcc gat gcc acg ggt gcg tat ttc ccg ctg gcc tgc acg 931Thr Gly Phe Ala Asp Ala Thr Gly Ala Tyr Phe Pro Leu Ala Cys Thr
265 270 275ctt aat ggc gca ccg gtg ttg gaa ttc ggc cgc cgc att ctg ggc gtg 979Leu Asn Gly Ala Pro Val Leu Glu Phe Gly Arg Arg Ile Leu Gly Val
280 285 290gaa tgg gaa gag ttc gat gcg ctt gca ctg gct gct caa ccc ggt tca 1027Glu Trp Glu Glu Phe Asp Ala Leu Ala Leu Ala Ala Gln Pro Gly Ser
295 300 305ggt ggc gtg acg ctc cag cct tat ttg gag ggc gag cgt acg ccg aat 1075Gly Gly Val Thr Leu Gln Pro Tyr Leu Glu Gly Glu Arg Thr Pro Asn310 315 320 325cgt ccc gca gca cgt ggc gtt ttg gct gga cta aac tgt gca acg acc 1123Arg Pro Ala Ala Arg Gly Val Leu Ala Gly Leu Asn Gys Ala Thr Thr
330 335 340cgc gag gac ttt gcc cga gca act gtt gaa ggc ttg ttg ttg gca ttg 1171Arg Glu Asp Phe Ala Arg Ala Thr Val Glu Gly Leu Leu Leu Ala Leu
345 350 355gat gat gct gta acg gcg ctg gtt gag gcc acg gga gtg ccc gtt cag 1219Asp Asp Ala Val Thr Ala Leu Val Glu Ala Thr Gly Val Pro Val Gln
360 365 370cgc atc cag ctc atc ggt ggc ggc gcg cgt tca cag gcg gtt cgt gag 1267Arg Ile Gln Leu Ile Gly Gly Gly Ala Arg Ser Gln Ala Val Arg Glu
375 380 385att gcc cct gag att ttc ggc cat gag att gtg gtt cca gaa ccc gct 1315Ile Ala Pro Glu Ile Phe Gly His Glu Ile Val Val Pro Glu Pro Ala390 395 400 405gaa tat gtg gcg ttg ggt gca gct cgt cag gcg gga tgg gcg ctg tcg 1363Glu Tyr Val Ala Leu Gly Ala Ala Arg Gln Ala Ala Trp Ala Leu Ser
410 415 420ggt gag gcc acg cca ccg cag tgg cca act ccc ggt tcc gat ccg cac 1411Gly Glu Ala Thr Pro Pro Gln Trp Pro Thr Pro Gly Ser Asp Pro His
425 430 435cgc gca cct aaa aac act gag ctg agc acg cgt tat gcg aag ctg cgt 1459Arg Ala Pro Lys Asn Thr Glu Leu Ser Thr Arg Tyr Ala Lys Leu Arg
440 445 450gct gca acg cag ggt tgg tac tagagctcga tattgtcgat caa 1503Ala Ala Thr Gln Gly Trp Tyr
455 460<210>386<211>460<212>PRT<213>谷氨酸棒杆菌<400>386Met Ala Leu Val Leu Gly Ile Asp Ser Ser Thr Gln Ser Cys Lys Ala1 5 10 15Leu Leu Val Asp Ala Ala Thr Gly Gln Val Ile Asp Glu Gly Arg Ala
20 25 30Ser His Pro Ser Gly Ser Glu Val Asp Pro Arg Ala Trp Ile Ala Ala
35 40 45Leu Asp Gln Ala Thr Glu Gly Leu Leu Glu Arg Ala Asp Ala Val Ser
50 55 60Ile Ala Gly Gln Gln His Gly Met Val Ala Leu Asp Glu Asn Asp Glu65 70 75 80Ile Val Arg Pro Ala Leu Leu Trp Asn Asp Thr Arg Ser Ala Gln Ala
85 90 95Ala Leu Asp Leu Asn Glu Glu Ile Gly Gly Asp Gln Ala Ala Val Asp
100 105 110Ala Thr Gly Ser Val Tyr Val Ala Ser Leu Thr Ala Thr Lys Met Arg
115 120 125Trp Met Arg Asp His Glu Pro Glu Asn Ala Ala Arg Thr Ala Ser Val
130 135 140Met Leu Pro His Asp Phe Leu Thr Trp His Leu Met Gly Arg Gly Arg145 150 155 160Lys Val Thr Asp His Gly Asp Ala Ser Gly Thr Gly Tyr Tyr Ser Thr
165 170 175Arg Asp Arg Ala Trp Arg Thr Asp Leu Ala Ala Leu Ala Leu Gly His
180 185 190Glu Val Glu Leu Pro Glu Leu Leu Ala Pro Asn Ala Ile Ala Gly Thr
195 200 205Thr Pro Gly Gly Val Lys Val Ala Ala Gly Thr Gly Asp Asn Ala Ala
210 215 220Ala Ala Leu Gly Leu Asp Leu Gln Pro Gly Asp Val Ser Val Ser Ile225 230 235 240Gly Thr Ser Gly Val Ala Gly Met Thr Val Gln His Ser Val His Asp
245 250 255Pro Ser Gly Leu Val Thr Gly Phe Ala Asp Ala Thr Gly Ala Tyr Phe
260 265 270Pro Leu Ala Cys Thr Leu Asn Gly Ala Pro Val Leu Glu Phe Gly Arg
275 280 285Arg Ile Leu Gly Val Glu Trp Glu Glu Phe Asp Ala Leu Ala Leu Ala
290 295 300Ala Gln Pro Gly Ser Gly Gly Val Thr Leu Gln Pro Tyr Leu Glu Gly305 310 315 320Glu Arg Thr Pro Asn Arg Pro Ala Ala Arg Gly Val Leu Ala Gly Leu
325 330 335Asn Cys Ala Thr Thr Arg Glu Asp Phe Ala Arg Ala Thr Val Glu Gly
340 345 350Leu Leu Leu Ala Leu Asp Asp Ala Val Thr Ala Leu Val Glu Ala Thr
355 360 365Gly Val Pro Val Gln Arg Ile Gln Leu Ile Gly Gly Gly Ala Arg Ser
370 375 380Gln Ala Val Arg Glu Ile Ala Pro Glu Ile Phe Gly His Glu Ile Val385 390 395 400Val Pro Glu Pro Ala Glu Tyr Val Ala Leu Gly Ala Ala Arg Gln Ala
405 410 415Ala Trp Ala Leu Ser Gly Glu Ala Thr Pro Pro Gln Trp Pro Thr Pro
420 425 430Gly Ser Asp Pro His Arg Ala Pro Lys Asn Thr Glu Leu Ser Thr Arg
435 440 445Tyr Ala Lys Leu Arg Ala Ala Thr Gln Gly Trp Tyr
450 455 460<210>387<211>1139<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1116)<223>FRXA0l927<400>387aat gac act cgt tct gcc cag gct gcg ttg gat ctc aat gag gag atc 48Asn Asp Thr Arg Ser Ala Gln Ala Ala Leu Asp Leu Asn Glu Glu Ile1 5 10 15ggc ggc gat cag gct gcg gta gat gcc acg gga agt gtg tat gtt gct 96Gly Gly Asp Gln Ala Ala Val Asp Ala Thr Gly Ser Val Tyr Val Ala
20 25 30tct tta act gcc acc aaa atg cgg tgg atg cgt gat cat gaa cca gaa 144Ser Leu Thr Ala Thr Lys Met Arg Trp Met Arg Asp His Glu Pro Glu
35 40 45aat gca gcg cgc acg gcg tcg gtg atg ttg cct cat gat ttc ctc acc 192Asn Ala Ala Arg Thr Ala Ser Val Met Leu Pro His Asp Phe Leu Thr
50 55 60tgg cat ttg atg gga cgc gga cgc aaa gtc acc gac cat ggt gat gct 240Trp His Leu Met Gly Arg Gly Arg Lys Val Thr Asp His Gly Asp Ala65 70 75 80tct gga acg ggc tac tac agc acg cgt gat cgt gcg tgg cgc acc gat 288Ser Gly Thr Gly Tyr Tyr Ser Thr Arg Asp Arg Ala Trp Arg Thr Asp
85 90 95cta gct gcc ttg gcg ctg ggc cat gag gtg gaa ctt cct gaa ctc ctg 336Leu Ala Ala Leu Ala Leu Gly His Glu Val Glu Leu Pro Glu Leu Leu
100 105 110gcc cca aat gcg att gcg gga aca act cca ggt gga gtg aaa gtt gct 384Ala Pro Asn Ala Ile Ala Gly Thr Thr Pro Gly Gly Val Lys Val Ala
115 120 125gca ggc acg gga gat aat gct gcg gct gcg ctt ggc ctt gat ttg cag 432Ala Gly Thr Gly Asp Asn Ala Ala Ala Ala Leu Gly Leu Asp Leu Gln
130 135 140cct ggt gat gtc agc gtg tcg atc ggc acc tct ggc gtt gcc ggc atg 480Pro Gly Asp Val Ser Val Ser Ile Gly Thr Ser Gly Val Ala Gly Met145 150 155 160acc gtt caa cat agc gtc cac gat cca tct ggt ttg gtc act ggt ttc 528Thr Val Gln His Ser Val His Asp Pro Ser Gly Leu Val Thr Gly Phe
165 170 175gcc gat gcc acg ggt gcg tat ttc ccg ctg gcc tgc acg ctt aat ggc 576Ala Asp Ala Thr Gly Ala Tyr Phe Pro Leu Ala Cys Thr Leu Asn Gly
180 185 190gca ccg gtg ttg gaa ttc ggc cgc cgc att ctg ggc gtg gaa tgg gaa 624Ala Pro Val Leu Glu Phe Gly Arg Arg Ile Leu Gly Val Glu Trp Glu
195 200 205gag ttc gat gcg ctt gca ctg gct gct caa ccc ggt tca ggt ggc gtg 672Glu Phe Asp Ala Leu Ala Leu Ala Ala Gln Pro Gly Ser Gly Gly Val
210 215 220acg ctc cag cct tat ttg gag ggc gag cgt acg ccg aat cgt ccc gca 720Thr Leu Gln Pro Tyr Leu Glu Gly Glu Arg Thr Pro Asn Arg Pro Ala225 230 235 240gca cgt ggc gtt ttg gct gga cta aac tgt gca acg acc cgc gag gac 768Ala Arg Gly Val Leu Ala Gly Leu Asn Cys Ala Thr Thr Arg Glu Asp
245 250 255ttt gcc cga gca act gtt gaa ggc ttg ttg ttg gca ttg gat gat gct 816Phe Ala Arg Ala Thr Val Glu Gly Leu Leu Leu Ala Leu Asp Asp Ala
260 265 270gta acg gcg ctg gtt gag gcc acg gga gtg ccc gtt cag cgc atc cag 864Val Thr Ala Leu Val Glu Ala Thr Gly Val Pro Val Gln Arg Ile Gln
275 280 285ctc atc ggt ggc ggc gcg cgt tca cag gcg gtt cgt gag att gcc cct 912Leu Ile Gly Gly Gly Ala Arg Ser Gln Ala Val Arg Glu Ile Ala Pro
290 295 300gag att ttc ggc cat gag att gtg gtt cca gaa ccc got gaa tat gtg 960Glu Ile Phe Gly His Glu Ile Val Val Pro Glu Pro Ala Glu Tyr Val305 310 315 320gcg ttg ggt gca gct cgt cag gcg gca tgg gcg ctg tcg ggt gag gcc 1008Ala Leu Gly Ala Ala Arg Gln Ala Ala Trp Ala Leu Ser Gly Glu Ala
325 330 335acg cca ccg cag tgg cca act ccc ggt tcc gat ccg cac cgc gca cct 1056Thr Pro Pro Gln Trp Pro Thr Pro Gly Ser Asp Pro His Arg Ala Pro
340 345 350aaa aac act gag ctg agc acg cgt tat gcg aag ctg cgt gct gca acg 1104Lys Asn Thr Glu Leu Ser Thr Arg Tyr Ala Lys Leu Arg Ala Ala Thr
355 360 365cag ggt tgg tac tagagctcga tattgtcgat caa 1139Gln Gly Trp Tyr
370<210>388<211>372<212>PRT<213>谷氨酸棒杆菌<400>388Asn Asp Thr Arg Ser Ala Gln Ala Ala Leu Asp Leu Asn Glu Glu Ile1 5 10 15Gly Gly Asp Gln Ala Ala Val Asp Ala Thr Gly Ser Val Tyr Val Ala
20 25 30Ser Leu Thr Ala Thr Lys Met Arg Trp Met Arg Asp His Glu Pro Glu
35 40 45Asn Ala Ala Arg Thr Ala Ser Val Met Leu Pro His Asp Phe Leu Thr
50 55 60Trp His Leu Met Gly Arg Gly Arg Lys Val Thr Asp His Gly Asp Ala65 70 75 80Ser Gly Thr Gly Tyr Tyr Ser Thr Arg Asp Arg Ala Trp Arg Thr Asp
85 90 95Leu Ala Ala Leu Ala Leu Gly His Glu Val Glu Leu Pro Glu Leu Leu
100 105 110Ala Pro Asn Ala Ile Ala Gly Thr Thr Pro Gly Gly Val Lys Val Ala
115 120 125Ala Gly Thr Gly Asp Asn Ala Ala Ala Ala Leu Gly Leu Asp Leu Gln
130 135 140Pro Gly Asp Val Ser Val Ser Ile Gly Thr Ser Gly Val Ala Gly Met145 150 155 160Thr Val Gln His Ser Val His Asp Pro Ser Gly Leu Val Thr Gly Phe
165 170 175Ala Asp Ala Thr Gly Ala Tyr Phe Pro Leu Ala Cys Thr Leu Asn Gly
180 185 190Ala Pro Val Leu Glu Phe Gly Arg Arg Ile Leu Gly Val Glu Trp Glu
195 200 205Glu Phe Asp Ala Leu Ala Leu Ala Ala Gln Pro Gly Ser Gly Gly Val
210 215 220Thr Leu Gln Pro Tyr Leu Glu Gly Glu Arg Thr Pro Asn Arg Pro Ala225 230 235 240Ala Arg Gly Val Leu Ala Gly Leu Asn Cys Ala Thr Thr Arg Glu Asp
245 250 255Phe Ala Arg Ala Thr Val Glu Gly Leu Leu Leu Ala Leu Asp Asp Ala
260 265 270Val Thr Ala Leu Val Glu Ala Thr Gly Val Pro Val Gln Arg Ile Gln
275 280 285Leu Ile Gly Gly Gly Ala Arg Ser Gln Ala Val Arg Glu Ile Ala Pro
290 295 300Glu Ile Phe Gly His Glu Ile Val Val Pro Glu Pro Ala Glu Tyr Val305 310 315 320Ala Leu Gly Ala Ala Arg Gln Ala Ala Trp Ala Leu Ser Gly Glu Ala
325 330 335Thr Pro Pro Gln Trp Pro Thr Pro Gly Ser Asp Pro His Arg Ala Pro
340 345 350Lys Asn Thr Glu Leu Ser Thr Arg Tyr Ala Lys Leu Arg Ala Ala Thr
355 360 365Gln Gly Trp Tyr
370<210>389<211>844<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(844)<223>RXA02729<400>389gaattaattg caggcacggt acctagcgtg acgaaaacta cgatccccac tgcccttatt 60catcgtgaat caatcatcaa ctccacttta aggaagaagg atg gac tcc cca atg 115
Met Asp Ser Pro Met
1 5agt aac tca acc ggt acc gac att gtc gtt gtc gga tcc atc aat gcc 163Ser Asn Ser Thr Gly Thr Asp Ile Val Val Val Gly Ser Ile Asn Ala
10 15 20gat ctc acc gca aaa gtt caa cgc cac cct gaa cct gga gaa acc ctc 211Asp Leu Thr Ala Lys Val Gln Arg His Pro Glu Pro Gly Glu Thr Leu
25 30 35ctg ggt agc ggc ggc aca gtg agt gct ggt ggc aaa ggc gcc aac caa 259Leu Gly Ser Gly Gly Thr Val Ser Ala Gly Gly Lys Gly Ala Asn Gln
40 45 50gct gtg gcg gca gcg caa tta ggt gcc aaa gtc acc atg atc ggt gcg 307Ala Val Ala Ala Ala Gln Leu Gly Ala Lys Val Thr Met Ile Gly Ala
55 60 65gtc gga acc gat caa atg gct ggc gag gcg ctg aca cat ttg cgt caa 355Val Gly Thr Asp Gln Met Ala Gly Glu Ala Leu Thr His Leu Arg Gln70 75 80 85tca gga gca gat atg tcc gcg att gcc act gtg gac ggt ccc act ggt 403Ser Gly AIa Asp Met Ser Ala Ile Ala Thr Val Asp Gly Pro Thr Gly
90 95 100ctt gcc atc atc act gtg tct gac gat ggg gaa aac acc atc atc gtt 451Leu Ala Ile Ile Thr Val Ser Asp Asp Gly Glu Asn Thr Ile Ile Val
105 110 115atc cct ggc gct aac gct tct gtc acc gcg gaa ttt gtt gat aaa cac 499Ile Pro Gly Ala Asn Ala Ser Val Thr Ala Glu Phe Val Asp Lys His
120 125 130tcc caa ctc att gag aac gcc ggc att gtg ttg ctt cag ggt gag atc 547Ser Gln Leu Ile Glu Asn Ala Gly Ile Val Leu Leu Gln Gly Glu Ile
135 140 145cct gcc gat ggt ttc gag cgt gcc gtt gat ctt tca caa gga cgt gtg 595Pro Ala Asp Gly Phe Glu Arg Ala Val Asp Leu Ser Gln Gly Arg Val150 155 160 165gtg atc aat ctg gct cca gtt gtg ccc gtg gga cat gat cag ctg cgt 643Val Ile Asn Leu Ala Pro Val Val Pro Val Gly His Asp Gln Leu Arg
170 175 180cgt gcc gat cca ttg ctg gtc aac gaa cac gaa ggc gct ctg gtg ctg 691Arg Ala Asp Pro Leu Leu Val Asn Glu His Glu Gly Ala Leu Val Leu
185 190 195gac atg ctt gga act cca gcg acc acg tct gat ccc caa agt ttg gtc 739Asp Met Leu Gly Thr Pro Ala Thr Thr Ser Asp Pro Gln Ser Leu Val
200 205 210act gaa ttg ctg gag cag ggt ttt act tcc gtg gtg atg aca ctt ggt 787Thr Glu Leu Leu Glu Gln Gly Phe Thr Ser Val Val Met Thr Leu Gly
215 220 225gcc gaa ggt gct ctg gtt ggc acg ccg ggc caa ctc acg gca att cct 835Ala Glu Gly Ala Leu Val Gly Thr Pro Gly Gln Leu Thr Ala Ile Pro230 235 240 245acc cca aag 844Thr Pro Lys<210>390<211>248<212>PRT<213>谷氨酸棒杆菌<400>390Met Asp Ser Pro Met Ser Asn Ser Thr Gly Thr Asp Ile Val Val Val1 5 10 15Gly Ser Ile Asn Ala Asp Leu Thr Ala Lys Val Gln Arg His Pro Glu
20 25 30Pro Gly Glu Thr Leu Leu Gly Ser Gly Gly Thr Val Ser Ala Gly Gly
35 40 45Lys Gly Ala Asn Gln Ala Val Ala Ala Ala Gln Leu Gly Ala Lys Val
50 55 60Thr Met Ile Gly Ala Val Gly Thr Asp Gln Met Ala Gly Glu Ala Leu65 70 75 80Thr His Leu Arg Gln Ser Gly Ala Asp Met Ser Ala Ile Ala Thr Val
85 90 95Asp Gly Pro Thr Gly Leu Ala Ile Ile Thr Val Ser Asp Asp Gly Glu
100 105 110Asn Thr Ile Ile Val Ile Pro Gly Ala Asn Ala Ser Val Thr Ala Glu
115 120 125Phe Val Asp Lys His Ser Gln Leu Ile Glu Asn Ala Gly Ile Val Leu
130 135 140Leu Gln Gly Glu Ile Pro Ala Asp Gly Phe Glu Arg Ala Val Asp Leu145 150 155 160Ser Gln Gly Arg Val Val Ile Asn Leu Ala Pro Val Val Pro Val Gly
165 170 175His Asp Gln Leu Arg Arg Ala Asp Pro Leu Leu Val Asn Glu His Glu
180 185 190Gly Ala Leu Val Leu Asp Met Leu Gly Thr Pro Ala Thr Thr Ser Asp
195 200 205Pro Gln Ser Leu Val Thr Glu Leu Leu Glu Gln Gly Phe Thr Ser Val
210 215 220Val Met Thr Leu Gly Ala Glu Gly Ala Leu Val Gly Thr Pro Gly Gln225 230 235 240Leu Thr Ala Ile Pro Thr Pro Lys
245<210>391<211>1026<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1003)<223>RXA02797<400>391acagtctcat gaagccataa taaccacctt ctacaaagat cgacgtagaa tggaataacc 60ccttatgaaa acgtttgcat aactccgcta aggatgttcc atg aat aat cga att 115
Met Asn Asn Arg Ile
1 5gtc gta gtc ggc tcc atc aac gct gat ctt aat gtt ctc gtt gac cgc 163Val Val Val Gly Ser Ile Asn Ala Asp Leu Asn Val Leu Val Asp Arg
10 15 20cac cca gca cct ggc gaa aca ctg ttg ggc agt ggt gga cac atc act 211His Pro Ala Pro Gly Glu Thr Leu Leu Gly Ser Gly Gly His Ile Thr
25 30 35gca gga ggc aaa ggc gcc aac cag gca gta gct gcc gct ctt caa ggt 259Ala Gly Gly Lys Gly Ala Asn Gln Ala Val Ala Ala Ala Leu Gln Gly
40 45 50gca gac gtc gcc ttt gtc ggc gct gtg ggc aag gat cct tac gct gcc 307Ala Asp Val Ala Phe Val Gly Ala Val Gly Lys Asp Pro Tyr Ala Ala
55 60 65cca gcc tta gaa ttc ctt cgt tcg tca ggc gtc gac ctt acg gca gta 355Pro Ala Leu Glu Phe Leu Arg Ser Ser Gly Val Asp Leu Thr Ala Val70 75 80 85tcc gaa gta gat gac acc acc ggg ctt gca gtt atc acc gtt gcc aaa 403Ser Glu Val Asp Asp Thr Thr Gly Leu Ala Val Ile Thr Val Ala Lys
90 95 100gac ggc gag aac aat atc gtt gtc atc ccc ggc gcg aat tcc ctg gtc 451Asp Gly Glu Asn Asn Ile Val Val Ile Pro Gly Ala Asn Ser Leu Val
105 110 115aat tgt gat tat gta agc agc caa tcc gct ctt tta gct gaa gct gga 499Asn Cys Asp Tyr Val Ser Set Gln Ser Ala Leu Leu Ala Glu Ala Gly
120 125 130atc ctg ttg ctg caa ggt gag atc cct gcg gat ggc ttc aaa gag gcc 547Ile Leu Leu Leu Gln Gly Glu Ile Pro Ala Asp Gly Phe Lys Glu Ala
135 140 145att cac cac acc atg ggt cgc gtc gtg gtg aat cta gcg ccc gtc atc 595Ile His His Thr Met Gly Arg Val Val Val Asn Leu Ala Pro Val Ile150 155 160 165gag gta gag aag tcc gcg tta ctt gag gct gat ccg atc atc gcc aat 643Glu Val Glu Lys Ser Ala Leu Leu Glu Ala Asp Pro Ile Ile Ala Asn
170 175 180gag cac gag gcc ggc ctg att ctg gat caa ttc ggg gca ggc atc gat 691Glu His Glu Ala Gly Leu Ile Leu Asp Gln Phe Gly Ala Gly Ile Asp
185 190 195tcc atg gat ccc cac gag ctc gcg caa gct ctc ctc gac gcc ggt ttc 739Ser Met Asp Pro His Glu Leu Ala Gln Ala Leu Leu Asp Ala Gly Phe
200 205 210gcc tct gtt gtt tta acg ctt gga tcc gca ggc gcg ttg gtc gcc gat 787Ala Ser Val Val Leu Thr Leu Gly Ser Ala Gly Ala Leu Val Ala Asp
215 220 225gcc acc ggt atc acg gac atc gcc aca cca acg gtg cag gca gtt gac 835Ala Thr Gly Ile Thr Asp Ile Ala Thr Pro Thr Val Gln Ala Val Asp230 235 240 245acc acg gga gcc ggt gac gct ttt gcc gga gcc ttc tgc gca cga cta 883Thr Thr Gly Ala Gly Asp Ala Phe Ala Gly Ala Phe Cys Ala Arg Leu
250 255 260att aaa ggc gat tcg ctt atc gac gcc gcc acc cac gca gca cgc gtc 931Ile Lys Gly Asp Ser Leu Ile Asp Ala Ala Thr His Ala Ala Arg Val
265 270 275ggc gct tac tcg gtg caa acc gcc gga gcg caa gcg tcc tat ccg gac 979Gly Ala Tyr Ser Val Gln Thr Ala Gly Ala Gln Ala Ser Tyr Pro Asp
280 285 290gcg agc gtt tca ctt ccc tct gtt taaaaaaact atttaagaag agg 1026Ala Ser Val Ser Leu Pro Ser Val
295 300<210>392<211>301<212>PRT<213>谷氨酸棒杆菌<400>392Met Asn Asn Arg Ile Val Val Val Gly Ser Ile Asn Ala Asp Leu Asn1 5 10 15Val Leu Val Asp Arg His Pro Ala Pro Gly Glu Thr Leu Leu Gly Ser
20 25 30Gly Gly His Ile Thr Ala Gly Gly Lys Gly Ala Asn Gln Ala Val Ala
35 40 45Ala Ala Leu Gln Gly Ala Asp Val Ala Phe Val Gly Ala Val Gly Lys
50 55 60Asp Pro Tyr Ala Ala Pro Ala Leu Glu Phe Leu Arg Ser Ser Gly Val65 70 75 80Asp Leu Thr Ala Val Ser Glu Val Asp Asp Thr Thr Gly Leu Ala Val
85 90 95Ile Thr Val Ala Lys Asp Gly Glu Asn Asn Ile Val Val Ile Pro Gly
100 105 110Ala Asn Ser Leu Val Asn Cys Asp Tyr Val Ser Ser Gln Ser Ala Leu
115 120 125Leu Ala Glu Ala Gly Ile Leu Leu Leu Gln Gly Glu Ile Pro Ala Asp
130 135 140Gly Phe Lys Glu Ala Ile His His Thr Met Gly Arg Val Val Val Asn145 150 155 160Leu Ala Pro Val Ile Glu Val Glu Lys Ser Ala Leu Leu Glu Ala Asp
165 170 175Pro Ile Ile Ala Asn Glu His Glu Ala Gly Leu Ile Leu Asp Gln Phe
180 185 190Gly Ala Gly Ile Asp Ser Met Asp Pro His Glu Leu Ala Gln Ala Leu
195 200 205Leu Asp Ala Gly Phe Ala Ser Val Val Leu Thr Leu Gly Ser Ala Gly
210 215 220Ala Leu Val Ala Asp Ala Thr Gly Ile Thr Asp Ile Ala Thr Pro Thr225 230 235 240Val Gln Ala Val Asp Thr Thr Gly Ala Gly Asp Ala Phe Ala Gly Ala
245 250 255Phe Cys Ala Arg Leu Ile Lys Gly Asp Ser Leu Ile Asp Ala Ala Thr
260 265 270His Ala Ala Arg Val Gly Ala Tyr Ser Val Gln Thr Ala Gly Ala Gln
275 280 285Ala Ser Tyr Pro Asp Ala Ser Val Ser Leu Pro Ser Val
290 295 300<210>393<211>1161<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1138)<223>RXA02730<400>393ccaacatcgc cttgcacgta ataggttaaa acacaagtga atgtaatcgt ttgcagcaat 60cgattacata aaggtagata atgagataaa gcgaggcgct atg gcg acg gaa aaa 115
Met Ala Thr Glu Lys
1 5ttc cga ccg act ctt aaa gat gtc gct cgt caa gca ggt gtc tcc atc 163Phe Arg Pro Thr Leu Lys Asp Val Ala Arg Gln Ala Gly Val Ser Ile
10 15 20gcc aca gca tca cga gca cta gcg gat aat ccg gcg gtt gct gca tcg 211Ala Thr Ala Ser Arg Ala Leu Ala Asp Asn Pro Ala Val Ala Ala Ser
25 30 35act cgt gaa aga atc caa caa tta gcc tct gat ctg ggt tac cgg gcc 259Thr Arg Glu Arg Ile Gln Gln Leu Ala Ser Asp Leu Gly Tyr Arg Ala
40 45 50aat gct caa gct cgt gcg ctt cgc agt tct cgc agc aac acc att ggt 307Asn Ala Gln Ala Arg Ala Leu Arg Ser Ser Arg Ser Asn Thr Ile Gly
55 60 65gtg att gtt ccc agt ttg att aac cat tac ttc gcc gca atg gtt act 355Val Ile Val Pro Ser Leu Ile Asn His Tyr Phe Ala Ala Met Val Thr70 75 80 85gaa att caa agc acc gcc agc aaa gct gga ctt gcc acg att atc acc 403Glu Ile Gln Ser Thr Ala Ser Lys Ala Gly Leu Ala Thr Ile Ile Thr
90 95 100aac agc aat gaa gat gcg acc act atg tct ggg tct ttg gag ttt ctc 451Asn Ser Asn Glu Asp Ala Thr Thr Met Ser Gly Ser Leu Glu Phe Leu
105 110 115acc tcg cat ggt gtc gat gga atc atc tgc gta cct aat gag gaa tgc 499Thr Ser His Gly Val Asp Gly Ile Ile Cys Val Pro Asn Glu Glu Cys
120 125 130gcg aat caa cta gag gac ttg cag aag caa gga atg cca gtg gtg ttg 547Ala Asn Gln Leu Glu Asp Leu Gln Lys Gln Gly Met Pro Val Val Leu
135 140 145gtt gac cga gag ctt cca gga gac tcc acc atc cca acg gcg acc tct 595Val Asp Arg Glu Leu Pro Gly Asp Ser Thr Ile Pro Thr Ala Thr Ser150 155 160 165aac ccc caa cca gga atc gcc gca gca gta gaa ctc ctg gct cac aac 643Asn Pro Gln Pro Gly Ile Ala Ala Ala Val Glu Leu Leu Ala His Asn
170 175 180aac gcg ttg ccg att ggt tac ctc tca ggt ccc atg gac acc tca aca 691Asn Ala Leu Pro Ile Gly Tyr Leu Ser Gly Pro Met Asp Thr Ser Thr
185 190 195ggt aga gag cga tta gag gat ttc aaa gca gcc tgc gcc aac tcc aaa 739Gly Arg Glu Arg Leu Glu Asp Phe Lys Ala Ala Cys Ala Asn Ser Lys
200 205 210att ggc gaa cag ctc gtt ttt ctg ggt ggg tac gaa caa agc gtt gga 787Ile Gly Glu Gln Leu Val Phe Leu Gly Gly Tyr Glu Gln Ser Val Gly
215 220 225ttt gaa ggc gct acg aaa ttg ctc gat caa gga gct aaa act ctt ttt 835Phe Glu Gly Ala Thr Lys Leu Leu Asp Gln Gly Ala Lys Thr Leu Phe230 235 240 245gcc ggc gat tct atg atg acg atc ggt gtc att gaa gcc tgc cat aag 883Ala Gly Asp Ser Met Met Thr Ile Gly Val Ile Glu Ala Cys His Lys
250 255 260gct ggt ttg gtt atc ggc aag gat gtc agc gtg att ggt ttt gat aca 931Ala Gly Leu Val Ile Gly Lys Asp Val Ser Val Ile Gly Phe Asp Thr
265 270 275cat ccg ctt ttt gcc ctg caa cct cat ccg ttg aca gtg att gat caa 979His Pro Leu Phe Ala Leu Gln Pro His Pro Leu Thr Val Ile Asp Gln
280 285 290aat gta gaa caa cta gcc caa cga gca gtg tct atc ctc acc gaa tta 1027Asn Val Glu Gln Leu Ala Gln Arg Ala Val Ser Ile Leu Thr Glu Leu
295 300 305att gca ggc acg gta cct agc gtg acg aaa act acg atc ccc act gcc 1075Ile Ala Gly Thr Val Pro Ser Val Thr Lys Thr Thr Ile Pro Thr Ala310 315 320 325ctt att cat cgt gaa tca atc atc aac tcc act tta agg aag aag gat 1123Leu Ile His Arg Glu Ser Ile Ile Asn Ser Thr Leu Arg Lys Lys Asp
330 335 340gga ctc ccc aat gag taactcaacc ggtaccgaca ttg 1161Gly Leu Pro Asn Glu
345<210>394<211>346<212>PRT<213>谷氨酸棒杆菌<400>394Met Ala Thr Glu Lys Phe Arg Pro Thr Leu Lys Asp Val Ala Arg Gln1 5 10 15Ala Gly Val Ser Ile Ala Thr Ala Ser Arg Ala Leu Ala Asp Asn Pro
20 25 30Ala Val Ala Ala Ser Thr Arg Glu Arg Ile Gln Gln Leu Ala Ser Asp
35 40 45Leu Gly Tyr Arg Ala Asn Ala Gln Ala Arg Ala Leu Arg Ser Ser Arg
50 55 60Ser Asn Thr Ile Gly Val Ile Val Pro Ser Leu Ile Asn His Tyr Phe65 70 75 80Ala Ala Met Val Thr Glu Ile Gln Ser Thr Ala Ser Lys Ala Gly Leu
85 90 95Ala Thr Ile Ile Thr Asn Ser Asn Glu Asp Ala Thr Thr Met Ser Gly
100 105 110Ser Leu Glu Phe Leu Thr Ser His Gly Val Asp Gly Ile Ile Cys Val
115 120 125Pro Asn Glu Glu Cys Ala Asn Gln Leu Glu Asp Leu Gln Lys Gln Gly
130 135 140Met Pro Val Val Leu Val Asp Arg Glu Leu Pro Gly Asp Ser Thr Ile145 150 155 160Pro Thr Ala Thr Ser Asn Pro Gln Pro Gly Ile Ala Ala Ala Val Glu
165 170 175Leu Leu Ala His Asn Asn Ala Leu Pro Ile Gly Tyr Leu Ser Gly Pro
180 185 190Met Asp Thr Ser Thr Gly Arg Glu Arg Leu Glu Asp Phe Lys Ala Ala
195 200 205Cys Ala Asn Ser Lys Ile Gly Glu Gln Leu Val Phe Leu Gly Gly Tyr
210 215 220Glu Gln Ser Val Gly Phe Glu Gly Ala Thr Lys Leu Leu Asp Gln Gly225 230 235 240Ala Lys Thr Leu Phe Ala Gly Asp Ser Met Met Thr Ile Gly Val Ile
245 250 255Glu Ala Cys His Lys Ala Gly Leu Val Ile Gly Lys Asp Val Ser Val
260 265 270Ile Gly Phe Asp Thr His Pro Leu Phe Ala Leu Gln Pro His Pro Leu
275 280 285Thr Val Ile Asp Gln Asn Val Glu Gln Leu Ala Gln Arg Ala Val Ser
290 295 300Ile Leu Thr Glu Leu Ile Ala Gly Thr Val Pro Ser Val Thr Lys Thr305 310 315 320Thr Ile Pro Thr Ala Leu Ile His Arg Glu Ser Ile Ile Asn Ser Thr
325 330 335Leu Arg Lys Lys Asp Gly Leu Pro Asn Glu
340 345<210>395<211>483<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(460)<223>RXA02551<400>395gctgcacaaa ttccgcgaaa gggtatcacc atcgacatca cagatgaaga ccgtgagatc 60ttaaaaaaac accgtcgatt tcatttcctt ctcctattac atg tcc gta tgt gaa 115
Met Ser Val Cys Glu
1 5gcc cac aac ccc gaa aac tac tca acc ggt ggc ggt aac atc atc ggc 163Ala His Asn Pro Glu Asn Tyr Ser Thr Gly Gly Gly Asn Ile Ile Gly
10 15 20gga gtg gtc agc ccc act ctc gcg gct tcc gaa tgg ggt tgg caa gtt 211Gly Val Val Ser Pro Thr Leu Ala Ala Ser Glu Trp Gly Trp Gln Val
25 30 35gat ccc ctc ggt ttg cgc atc gtc ctg aac aac tac tgg gag cgc tgg 259Asp Pro Leu Gly Leu Arg Ile Val Leu Asn Asn Tyr Trp Glu Arg Trp
40 45 50cag aag cca ctg ttc atc gtc gaa aac gga cta gga gca aag gac gtg 307Gln Lys Pro Leu Phe Ile Val Glu Asn Gly Leu Gly Ala Lys Asp Val
55 60 65ctt atc gac gga ccc tcc ggc cca aca gta aac gat gac tac cgc atc 355Leu Ile Asp Gly Pro Ser Gly Pro Thr Val Asn Asp Asp Tyr Arg Ile70 75 80 85aaa tac ctc gac gac ggc ggc tca gga atc ttg aag cgc tac aag aag 403Lys Tyr Leu Asp Asp Gly Gly Ser Gly Ile Leu Lys Arg Tyr Lys Lys
90 95 100aag tcc ttt gat tgg tgc cgc gac atc atc gcc acc aat ggc gaa agc 451Lys Ser Phe Asp Trp Cys Arg Asp Ile Ile Ala Thr Asn Gly Glu Ser
105 110 115ctg gaa tcc taagaaataa aggtaggtgt cac 483Leu Glu Ser
120<210>396<211>120<212>PRT<213>谷氨酸棒杆菌<400>396Met Ser Val Cys Glu Ala His Asn Pro Glu Asn Tyr Ser Thr Gly Gly1 5 10 15Gly Asn Ile Ile Gly Gly Val Val Ser Pro Thr Leu Ala Ala Ser Glu
20 25 30Trp Gly Trp Gln Val Asp Pro Leu Gly Leu Arg Ile Val Leu Asn Asn
35 40 45Tyr Trp Glu Arg Trp Gln Lys Pro Leu Phe Ile Val Glu Asn Gly Leu
50 55 60Gly Als Lys Asp Val Leu Ile Asp Gly Pro Ser Gly Pro Thr Val Asn65 70 75 80Asp Asp Tyr Arg Ile Lys Tyr Leu Asp Asp Gly Gly Ser Gly Ile Leu
85 90 95Lys Arg Tyr Lys Lys Lys Ser Phe Asp Trp Cys Arg Asp Ile Ile Ala
100 105 110Thr Asn Gly Glu Ser Leu Glu Ser
115 120<210>397<211>795<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(772)<223>RXA01325<400>397gcgcagcgct cggacgatta cgaacttcag gagaactcgg ggtcattcgt tgcattctac 60cctggaaatt ttcccacact aagtcaggtc taagtagggt atg gat atg acg att 115
Met Asp Met Thr Ile
1 5tcc cgc tcc acc atg gcc caa atc ctt gac tac acc ctc ctc gga cca 163Ser Arg Ser Thr Met Ala Gln Ile Leu Asp Tyr Thr Leu Leu Gly Pro
10 15 20gaa gta acc aac tcc gaa ctg gcc gca ttt ata gat tcc gca att gag 211Glu Val Thr Asn Ser Glu Leu Ala Ala Phe Ile Asp Ser Ala Ile Glu
25 30 35ctg gga gtc ggc acg atc tgt gtc ccc aac agc atg gtc aac cta act 259Leu Gly Val Gly Thr Ile Cys Val Pro Asn Ser Met Val Asn Leu Thr
40 45 50gca aaa gcc caa gaa gct gga att cga gtg gcc acc gtc gca gga ttc 307Ala Lys Ala Gln Glu Ala Gly Ile Arg Val Ala Thr Val Ala Gly Phe
55 60 65ccg cac ggc aaa acc ccc gcg ttg gtg aaa gcc gcc gaa gcg cgc ctt 355Pro His Gly Lys Thr Pro Ala Leu Val Lys Ala Ala Glu Ala Arg Leu70 75 80 85gcc gta cag tcc gga gct tcc gaa gta gat gtt gtt ttg gat att gcg 403Ala Val Gln Ser Gly Ala Ser Glu Val Asp Val Val Leu Asp Ile Ala
90 95 100gta gtg aaa gag gga gat gcc aat agg ttg ctg cag gaa att gtg gca 451Val Val Lys Glu Gly Asp Ala Asn Arg Leu Leu Gln Glu Ile Val Ala
105 110 115atc agg gag gct gtt cca tct cct gtg gtg ctg aaa ttc atc ctc gaa 499Ile Arg Glu Ala Val Pro Ser Pro Val Val Leu Lys Phe Ile Leu Glu
120 125 130aca gct gtt gtg agt gat gaa gca att gtg act gca gtg aat gcg ttg 547Thr Ala Val Val Ser Asp Glu Ala Ile Val Thr Ala Val Asn Ala Leu
135 140 145att gct gct ggt gct gac ttc gct aaa act tcc acg gga ttc cac cca 595Ile Ala Ala Gly Ala Asp Phe Ala Lys Thr Ser Thr Gly Phe His Pro150 155 160 165gcg gga ggg gca act gtt gag gct gtt cgg gtg atg gct tcg gct tct 643Ala Gly Gly Ala Thr Val Glu Ala Val Arg Val Met Ala Ser Ala Ser
170 175 180cgg gga agg gtt gga att aag gct gcc ggt ggg gtg aaa act tgg gaa 691Arg Gly Arg Val Gly Ile Lys Ala Ala Gly Gly Val Lys Thr Trp Glu
185 190 195gat gcg gtg gcg ttt gtt gaa gca ggg gct act cgc att gga act tct 739Asp Ala Val Ala Phe Val Glu Ala Gly Ala Thr Arg Ile Gly Thr Ser
200 205 210aat gcg gga gcc att ttg gag ggt gcg ccg gag tagtttggcg ttctaatcgg 792Asn Ala Gly Ala Ile Leu Glu Gly Ala Pro Glu
215 220gac 795<210>398<211>224<212>PRT<213>谷氨酸棒杆菌<400>398Met Asp Met Thr Ile Ser Arg Ser Thr Met Ala Gln Ile Leu Asp Tyr1 5 10 15Thr Leu Leu Gly Pro Glu Val Thr Asn Ser Glu Leu Ala Ala Phe Ile
20 25 30Asp Ser Ala Ile Glu Leu Gly Val Gly Thr Ile Cys Val Pro Asn Ser
35 40 45Met Val Asn Leu Thr Ala Lys Ala Gln Glu Ala Gly Ile Arg Val Ala
50 55 60Thr Val Ala Gly Phe Pro His Gly Lys Thr Pro Ala Leu Val Lys Ala65 70 75 80Ala Glu Ala Arg Leu Ala Val Gln Ser Gly Ala Ser Glu Val Asp Val
85 90 95Val Leu Asp Ile Ala Val Val Lys Glu Gly Asp Ala Asn Arg Leu Leu
100 105 110Gln Glu Ile Val Ala Ile Arg Glu Ala Val Pro Ser Pro Val Val Leu
115 120 125Lys Phe Ile Leu Glu Thr Ala Val Val Ser Asp Glu Ala Ile Val Thr
130 135 140Ala Val Asn Ala Leu Ile Ala Ala Gly Ala Asp Phe Ala Lys Thr Ser145 150 155 160Thr Gly Phe His Pro Ala Gly Gly Ala Thr Val Glu Ala Val Arg Val
165 170 175Met Ala Ser Ala Ser Arg Gly Arg Val Gly Ile Lys Ala Ala Gly Gly
180 185 190Val Lys Thr Trp Glu Asp Ala Val Ala Phe Val Glu Ala Gly Ala Thr
195 200 205Arg Ile Gly Thr Ser Asn Ala Gly Ala Ile Leu Glu Gly Ala Pro Glu
210 215 220<210> 399<211> 684<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(661)<223>RXA00195<400>399tcgcagtcat catgcaggca taacctgaaa cccatccgtt tggattgccc caaatgggtg 60tagtgggtgc gtttacccaa caagtgcaag aatgggagtc gtg act aaa aag atc 115
Val Thr Lys Lys Ile
1 5ctt att ttg gga agc act ggt tcg att gga act cag gcg ctg gac gtt 163Leu Ile Leu Gly Ser Thr Gly Ser Ile Gly Thr Gln Ala Leu Asp Val
10 15 20att gct gat aat tca gac aag ttt gag gtg gtg ggt atc gct gcg ggc 211Ile Ala Asp Asn Ser Asp Lys Phe Glu Val Val Gly Ile Ala Ala Gly
25 30 35ggt tct cag cca gac ctc gtt att tcg cag gcg cag cag ttg ggg ctg 259Gly Ser Gln Pro Asp Leu Val Ile Ser Gln Ala Gln Gln Leu Gly Leu
40 45 50gct gca gac aag gtt gcg gtt gct gat gca cag gct gcc gca gta att 307Ala Ala Asp Lys Val Ala Val Ala Asp Ala Gln Ala Ala Ala Val Ile
55 60 65tcg aag gct ctc ggc ggc gag atc atc tct gga acc gat gct gcg aag 355Ser Lys Ala Leu Gly Gly Glu Ile Ile Ser Gly Thr Asp Ala Ala Lys70 75 80 85att ctg gtg gaa acc aca aag gcc gac act gtg ctt aat gct ctg gtt 403Ile Leu Val Glu Thr Thr Lys Ala Asp Thr Val Leu Asn Ala Leu Val
90 95 100ggt tct ttg ggg ctt gcg gca acg ctg gcc act ctg gaa tct ggt gcg 451Gly Ser Leu Gly Leu Ala Ala Thr Leu Ala Thr Leu Glu Ser Gly Ala
105 110 115cat ctt gcc ttg gct aac aaa gaa tcg ctg gtt gcc ggt ggt gag ttt 499His Leu Ala Leu Ala Asn Lys Glu Ser Leu Val Ala Gly Gly Glu Phe
120 125 130gtt acc tca aag gca aag ctg ggg cag atc att ccg gtc gat tcg gag 547Val Thr Ser Lys Ala Lys Leu Gly Gln Ile Ile Pro Val Asp Ser Glu
135 140 145cac tct gcc atg gcg cag tgt ttg cgt tcg ggt act cgt gat gag gtt 595His Ser Ala Met Ala Gln Cys Leu Arg Ser Gly Thr Arg Asp Glu Val150 155 160 165gcg cgg att gtg ctg aca gct tcg ggc gga cct ttc agg gct gga cca 643Ala Arg Ile Val Leu Thr Ala Ser Gly Gly Pro Phe Arg Ala Gly Pro
170 175 180ggg aga aga tgt ggg agg tgactcccga gcaggcagca gcg 684Gly Arg Arg Cys Gly Arg
185<210>400<211>187<212>PRT<213>谷氨酸棒杆菌<400>400Val Thr Lys Lys Ile Leu Ile Leu Gly Ser Thr Gly Ser Ile Gly Thr1 5 10 15Gln Ala Leu Asp Val Ile Ala Asp Asn Ser Asp Lys Phe Glu Val Val
20 25 30Gly Ile Ala Ala Gly Gly Ser Gln Pro Asp Leu Val Ile Ser Gln Ala
35 40 45Gln Gln Leu Gly Leu Ala Ala Asp Lys Val Ala Val Ala Asp Ala Gln
50 55 60Ala Ala Ala Val Ile Ser Lys Ala Leu Gly Gly Glu Ile Ile Ser Gly65 70 75 80Thr Asp Ala Ala Lys Ile Leu Val Glu Thr Thr Lys Ala Asp Thr Val
85 90 95Leu Asn Ala Leu Val Gly Ser Leu Gly Leu Ala Ala Thr Leu Ala Thr
100 105 110Leu Glu Ser Gly Ala His Leu Ala Leu Ala Asn Lys Glu Ser Leu Val
115 120 125Ala Gly Gly Glu Phe Val Thr Ser Lys Ala Lys Leu Gly Gln Ile Ile
130 135 140Pro Val Asp Ser Glu His Ser Ala Met Ala Gln Cys Leu Arg Ser Gly145 150 155 160Thr Arg Asp Glu Val Ala Arg Ile Val Leu Thr Ala Ser Gly Gly Pro
165 170 175Phe Arg Ala Gly Pro Gly Arg Arg Cys Gly Arg
180 185<210>401<211>738<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(715)<223>RXA00l96<400>401ctgccatggc gcagtgtttg cgttcgggta ctcgtgatga ggttgcgcgg attgtgctga 60cagcttcggg cggacctttc agggctggac cagggagaag atg tgg gag gtg act 115
Met Trp Glu Val Thr
1 5ccc gag cag gca gca gcg cac cca acg tgg gcg atg ggg cag atg aac 163Pro Glu Gln Ala Ala Ala His Pro Thr Trp Ala Met Gly Gln Met Asn
10 15 20acg ttg aac tcc gcc acc ctt att aat aaa ggc ctc gaa ctc atc gag 211Thr Leu Asn Ser Ala Thr Leu Ile Asn Lys Gly Leu Glu Leu Ile Glu
25 30 35gcg acc ctg ctg ttt gaa acg gat gcg gat ctc att gat gtg acg gtg 259Ala Thr Leu Leu Phe Glu Thr Asp Ala Asp Leu Ile Asp Val Thr Val
40 45 50cat ccg cag tcg atc atc cac tcc atg atc acg ttt acg gat ggt gcg 307His Pro Gln Set Ile Ile His Set Met Ile Thr Phe Thr Asp Gly Ala
55 60 65acc atc gcg cag gcg tcg cca cca tcg atg aaa ctg ccg atc gcg ttg 355Thr Ile Ala Gln Ala Ser Pro Pro Ser Met Lys Leu Pro Ile Ala Leu70 75 80 85gcg ctt gat tgg cca cat cgg gtg ccg aag gct cag ccg gcg ctg gat 403Ala Leu Asp Trp Pro His Arg Val Pro Lys Ala Gln Pro Ala Leu Asp
90 95 100ttc acc gct gct cat acc tgg gct ttt gag ccg gtg gat gat gcc gca 451Phe Thr Ala Ala His Thr Trp Ala Phe Glu Pro Val Asp Asp Ala Ala
105 110 115ttc cct gcg gtg cag ctg gct agg cac gtc gca aag caa aaa ggc acg 499Phe Pro Ala Val Gln Leu Ala Arg His Val Ala Lys Gln Lys Gly Thr
120 125 130tac ccc gcg gtg tat aac gcc gcc aac gag gag gcg gct gag gcg ttt 547Tyr Pro Ala Val Tyr Asn Ala Ala Asn Glu Glu Ala Ala Glu Ala Phe
135 140 145ttg cgc ggg cga atc aag ttt ccg cag atc gtg gac gtg gtg gac gag 595Leu Arg Gly Arg Ile Lys Phe Pro Gln Ile Val Asp Val Val Asp Glu150 155 160 165gtc ctc caa gga gct tct cag ttt gct ggt gta gca tca cac gtc gat 643Val Leu Gln Gly Ala Ser Gln Phe Ala Gly Val Ala Ser His Val Asp
170 175 180gat att ttg gca acc gaa tct gag gca cgc gcg cgt gcg aat gct ttg 691Asp Ile Leu Ala Thr Glu Ser Glu Ala Arg Ala Arg Ala Asn Ala Leu
185 190 195atc aac cgg ttg gca acc aac ttg taagctaagg agcttccgcc tcg 738Ile Asn Arg Leu Ala Thr Asn Leu
200 205<210>402<211>205<212>PRT<213>谷氨酸棒杆菌<400>402Met Trp Glu Val Thr Pro Glu Gln Ala Ala Ala His Pro Thr Trp Ala1 5 10 15Met Gly Gln Met Asn Thr Leu Asn Ser Ala Thr Leu Ile Asn Lys Gly
20 25 30Leu Glu Leu Ile Glu Ala Thr Leu Leu Phe Glu Thr Asp Ala Asp Leu
35 40 45Ile Asp Val Thr Val His Pro Gln Ser Ile Ile His Ser Met Ile Thr
50 55 60Phe Thr Asp Gly Ala Thr Ile Ala Gln Ala Ser Pro Pro Ser Met Lys65 70 75 80Leu Pro Ile Ala Leu Ala Leu Asp Trp Pro His Arg Val Pro Lys Ala
85 90 95Gln Pro Ala Leu Asp Phe Thr Ala Ala His Thr Trp Ala Phe Glu Pro
100 105 110Val Asp Asp Ala Ala Phe Pro Ala Val Gln Leu Ala Arg His Val Ala
115 120 125Lys Gln Lys Gly Thr Tyr Pro Ala Val Tyr Asn Ala Ala Asn Glu Glu
130 135 140Ala Ala Glu Ala Phe Leu Arg Gly Arg Ile Lys Phe Pro Gln Ile Val145 150 155 160Asp Val Val Asp Glu Val Leu Gln Gly Ala Ser Gln Phe Ala Gly Val
165 170 175Ala Ser His Val Asp Asp Ile Leu Ala Thr Glu Ser Glu Ala Arg Ala
180 185 190Arg Ala Asn Ala Leu Ile Asn Arg Leu Ala Thr Asn Leu
195 200 205<210>403<211>2031<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2008)<223>RXN01562<400>403gcaggtgcac atttgttttg tcacctgcac aaaagtgtcg ccagcccgat acttgtacaa 60ccgtccgcat ccgagaagca aaggtgtctg actcgcgcca atg gga att ctg aac 115
Met Gly Ile Leu Asn
1 5agt att tca aca cct gct gac tta aag gcc ctt aat gat gag gat ttg 163Set Ile Ser Thr Pro Ala Asp Leu Lys Ala Leu Asn Asp Glu Asp Leu
10 15 20gac gct ctt gcc aaa gaa atc cga act ttc ctg gtc gat aaa gtc gca 211Asp Ala Leu Ala Lys Glu Ile Arg Thr Phe Leu Val Asp Lys Val Ala
25 30 35gca act ggt ggc cac tta ggt cca aat ttg ggc gta gtg gaa tta acc 259Ala Thr Gly Gly His Leu Gly Pro Asn Leu Gly Val Val Glu Leu Thr
40 45 50atc ggt ctt cat cga gtt ttc gat tcg cct caa gac ccg atc atc ttt 307Ile Gly Leu His Arg Val Phe Asp Ser Pro Gln Asp Pro Ile Ile Phe
55 60 65gat act tct cac cag tcc tat gtg cat aag atc ctg acg ggt cgc gct 355Asp Thr Ser His Gln Ser Tyr Val His Lys Ile Leu Thr Gly Arg Ala70 75 80 85aaa gat ttt gat tct ttg cgt caa aaa gat ggc ctt tct ggt tac acc 403Lys Asp Phe Asp Ser Leu Arg Gln Lys Asp Gly Leu Ser Gly Tyr Thr
90 95 100tgc cgt gct gaa agt gag cac gat tgg act gag tct tcg cat gct tcg 451Cys Arg Ala Glu Ser Glu His Asp Trp Thr Glu Ser Ser His Ala Ser
105 1l0 115gcg gcc ttg tct tat gcg gat ggt ttg tct aaa gcc aag cag ttg gat 499Ala Ala Leu Ser Tyr Ala Asp Gly Leu Ser Lys Ala Lys Gln Leu Asp
120 125 130ggc gat acc acg cat agt gtg gtt gct gtc gtt ggt gat ggc gct cta 547Gly Asp Thr Thr His Ser Val Val Ala Val Val Gly Asp Gly Ala Leu
135 140 145act ggc ggc atg tgt tgg gaa gca ctg aac aat att gct gct ggt aaa 595Thr Gly Gly Met Cys Trp Glu Ala Leu Asn Asn Ile Ala Ala Gly Lys150 155 160 165gac cgc aaa gtt gtt gtc gta gtc aat gac aat ggc cgg agt tat tct 643Asp Arg Lys Val Val Val Val Val Asn Asp Asn Gly Arg Ser Tyr Ser
170 175 180cca acc att ggc gga ttt ggg gaa aac ctt gcg ggc ctt cgc atg cag 691Pro Thr Ile Gly Gly Phe Ala Glu Asn Leu Ala Gly Leu Arg Met Gln
185 190 195cct ttc tat gat cgc ttc atg gaa aag ggc aag acg tcc ctg aaa tcc 739Pro Phe Tyr Asp Arg Phe Met Glu Lys Gly Lys Thr Ser Leu Lys Ser
200 205 210atg ggg tgg gta ggg gag cgt act ttt gaa gcg ctc cat gca ttt aaa 787Met Gly Trp Val Gly Glu Arg Thr Phe Glu Ala Leu His Ala Phe Lys
215 220 225gaa ggt gtg aag agc acc gtc att ccc acc gaa atg ttc cct gaa ctg 835Glu Gly Val Lys Ser Thr Val Ile Pro Thr Glu Met Phe Pro Glu Leu230 235 240 245ggc atg aaa tac gtg ggt ccg gtt gat gga cat aac caa aaa gct gtc 883Gly Met Lys Tyr Val Gly Pro Val Asp Gly His Asn Gln Lys Ala Val
250 255 260gac aat gcg ctg aaa tac gct cat gat tat gat ggc ccc atc atc gtg 931Asp Asn Ala Leu Lys Tyr Ala His Asp Tyr Asp Gly Pro Ile Ile Val
265 270 275cac atg gtc acc gaa aag ggt cgt ggt tac gcg cct gct gag cag gat 979His Met Val Thr Glu Lys Gly Arg Gly Tyr Ala Pro Ala Glu Gln Asp
280 285 290ttg gac gaa ttg atg cac tcc acg ggc gtc atc gat ccg ctc aca gga 1027Leu Asp Glu Leu Met His Ser Thr Gly Val Ile Asp Pro Leu Thr Gly
295 300 305gct cct aaa tct gca tca aag ccc ggt tgg acc tct gtg ttc agc gat 1075Ala Pro Lys Ser Ala Ser Lys Pro Gly Trp Thr Ser Val Phe Ser Asp310 315 320 325gag ctg gtc aag att ggt gcg cag aat gaa aac gtt gtt gcc atc acc 1123Glu Leu Val Lys Ile Gly Ala Gln Asn Glu Asn Val Val Ala Ile Thr
330 335 340gcc gcg atg gca ggt cct acc ggt ctg tcc aag ttc gaa gcc aat ttc 1171Ala Ala Met Ala Gly Pro Thr Gly Leu Ser Lys Phe Glu Ala Asn Phe
345 350 355ccc aac cga ttc ttt gat gtc ggc att gct gag cag cac gcg gta act 1219Pro Asn Arg Phe Phe Asp Val Gly Ile Ala Glu Gln His Ala Val Thr
360 365 370tct gcc gca ggc ctc gca ttg ggt gga aaa cac cct gtg gtg gct att 1267Ser Ala Ala Gly Leu Ala Leu Gly Gly Lys His Pro Val Val Ala Ile
375 380 385tac tcc acg ttc ttg aac cgc gct ttt gat cag ctg ctc atg gat gtg 1315Tyr Ser Thr Phe Leu Asn Arg Ala Phe Asp Gln Leu Leu Met Asp Val390 395 400 405ggc atg ctc aac cag cct gtt act ttg gtg ctt gat cgc tca ggt gtc 1363Gly Met Leu Asn Gln Pro Val Thr Leu Val Leu Asp Arg Ser Gly Val
410 415 420acg ggt tcg gat gga gcg agc cac aat ggc gtc tgg gat atg gcg ctg 1411Thr Gly Set Asp Gly Ala Ser His Asn Gly Val Trp Asp Met Ala Leu
425 430 435acc tcg atc gtt cca ggc gtg cag gtg gcg gca cca cgt gat gag gat 1459Thr Ser Ile Val Pro Gly Val Gln Val Ala Ala Pro Arg Asp Glu Asp
440 445 450tcc ttg cgt gag ctg ctc aat gag gct att tcc atc gat gat ggc ccc 1507Ser Leu Arg Glu Leu Leu Asn Glu Ala Ile Ser Ile Asp Asp Gly Pro
455 460 465aca gtt gtg cgt ttc ccc aag ggc gac ttg cca act cca att gtt gct 1555Thr Val Val Arg Phe Pro Lys Gly Asp Leu Pro Thr Pro Ile Val Ala470 475 480 485atc gac acc ttg gaa gac ggc gtg gat gtc ctc gca tat gaa gac gcc 1603Ile Asp Thr Leu Glu Asp Gly Val Asp Val Leu Ala Tyr Glu Asp Ala
490 495 500act gac gtt gaa tca acc gac gat gcg cca tca gtt ctc atc att gcg 1651Thr Asp Val Glu Ser Thr Asp Asp Ala Pro Ser Val Leu Ile Ile Ala
505 510 515gta ggc gag cgc gca act gtt gca ctt gac gtt gct tcc agg att aaa 1699Val Gly Glu Arg Ala Thr Val Ala Leu Asp Val Ala Ser Arg Ile Lys
520 525 530cag cac ggc gtg aac gtc acg gtt gtt gac ccc cgc tgg att gtc ccc 1747Gln His Gly Val Asn Val Thr Val Val Asp Pro Arg Trp Ile Val Pro
535 540 545atc ccg cag tcc ttg gtc gcg ctg tct gat gat cat gac ctc gtg atc 1795Ile Pro Gln Ser Leu Val Ala Leu Ser Asp Asp His Asp Leu Val Ile550 555 560 565acc atc gaa gac ggc gtc atc cac ggc ggc gtg gga tcc ttg ctc tct 1843Thr Ile Glu Asp Gly Val Ile His Gly Gly Val Gly Ser Leu Leu Ser
570 575 580gat gcg ctt aac gcc tct gag gtg gat acc cct cgc cga caa atc gcc 1891Asp Ala Leu Asn Ala Ser Glu Val Asp Thr Pro Arg Arg Gln Ile Ala
585 590 595gtg ccc cag aag tac ctg gat cac gcg tcc cgc aat gaa gtg ctc gcc 1939Val Pro Gln Lys Tyr Leu Asp His Ala Ser Arg Asn Glu Val Leu Ala
600 605 610gat tat ggc ctc gac gcc gac ggc att gaa acc act gtt gtt gga tgg 1987Asp Tyr Gly Leu Asp Ala Asp Gly Ile Glu Thr Thr Val Val Gly Trp
615 620 625ctg gat tcc ctg ttc ggg gaa taaaaccctg cttatcgacg ccg 2031Leu Asp Ser Leu Phe Gly Glu630 635<210>404<211>636<212>PRT<213>谷氨酸棒杆菌<400>404Met Gly Ile Leu Asn Ser Ile Ser Thr Pro Ala Asp Leu Lys Ala Leu1 5 10 15Asn Asp Glu Asp Leu Asp Ala Leu Ala Lys Glu Ile Arg Thr Phe Leu
20 25 30Val Asp Lys Val Ala Ala Thr Gly Gly His Leu Gly Pro Asn Leu Gly
35 40 45Val Val Glu Leu Thr Ile Gly Leu His Arg Val Phe Asp Ser Pro Gln
50 55 60Asp Pro Ile Ile Phe Asp Thr Ser His Gln Ser Tyr Val His Lys Ile65 70 75 80Leu Thr Gly Arg Ala Lys Asp Phe Asp Ser Leu Arg Gln Lys Asp Gly
85 90 95Leu Ser Gly Tyr Thr Cys Arg Ala Glu Ser Glu His Asp Trp Thr Glu
100 105 110Ser Ser His Ala Ser Ala Ala Leu Ser Tyr Ala Asp Gly Leu Ser Lys
115 120 125Ala Lys Gln Leu Asp Gly Asp Thr Thr His Ser Val Val Ala Val Val
130 135 140Gly Asp Gly Ala Leu Thr Gly Gly Met Cys Trp Glu Ala Leu Asn Asn145 150 155 160Ile Ala Ala Gly Lys Asp Arg Lys Val Val Val Val Val Asn Asp Asn
165 170 175Gly Arg Ser Tyr Ser Pro Thr Ile Gly Gly Phe Ala Glu Asn Leu Ala
180 185 190Gly Leu Arg Met Gln Pro Phe Tyr Asp Arg Phe Met Glu Lys Gly Lys
195 200 205Thr Ser Leu Lys Ser Met Gly Trp Val Gly Glu Arg Thr Phe Glu Ala
210 215 220Leu His Ala Phe Lys Glu Gly Val Lys Ser Thr Val Ile Pro Thr Glu225 230 235 240Met Phe Pro Glu Leu Gly Met Lys Tyr Val Gly Pro Val Asp Gly His
245 250 255Asn Gln Lys Ala Val Asp Asn Ala Leu Lys Tyr Ala His Asp Tyr Asp
260 265 270Gly Pro Ile Ile Val His Met Val Thr Glu Lys Gly Arg Gly Tyr Ala
275 280 285Pro Ala Glu Gln Asp Leu Asp Glu Leu Met His Ser Thr Gly Val Ile
290 295 300Asp Pro Leu Thr Gly Ala Pro Lys Ser Ala Ser Lys Pro Gly Trp Thr305 310 315 320Ser Val Phe Ser Asp Glu Leu Val Lys Ile Gly Ala Gln Asn Glu Asn
325 330 335Val Val Ala Ile Thr Ala Ala Met Ala Gly Pro Thr Gly Leu Ser Lys
340 345 350Phe Glu Ala Asn Phe Pro Asn Arg Phe Phe Asp Val Gly Ile Ala Glu
355 360 365Gln His Ala Val Thr Ser Ala Ala Gly Leu Ala Leu Gly Gly Lys His
370 375 380Pro Val Val Ala Ile Tyr Ser Thr Phe Leu Asn Arg Ala Phe Asp Gln385 390 395 400Leu Leu Met Asp Val Gly Met Leu Asn Gln Pro Val Thr Leu Val Leu
405 410 415Asp Arg Ser Gly Val Thr Gly Ser Asp Gly Ala Ser His Asn Gly Val
420 425 430Trp Asp Met Ala Leu Thr Ser Ile Val Pro Gly Val Gln Val Ala Ala
435 440 445Pro Arg Asp Glu Asp Ser Leu Arg Glu Leu Leu Asn Glu Ala Ile Ser
450 455 460Ile Asp Asp Gly Pro Thr Val Val Arg Phe Pro Lys Gly Asp Leu Pro465 470 475 480Thr Pro Ile Val Ala Ile Asp Thr Leu Glu Asp Gly Val Asp Val Leu
485 490 495Ala Tyr Glu Asp Ala Thr Asp Val Glu Ser Thr Asp Asp Ala Pro Ser
500 505 510Val Leu Ile Ile Ala Val Gly Glu Arg Ala Thr Val Ala Leu Asp Val
515 520 525Ala Ser Arg Ile Lys Gln His Gly Val Asn Val Thr Val Val Asp Pro
530 535 540Arg Trp Ile Val Pro Ile Pro Gln Ser Leu Val Ala Leu Ser Asp Asp545 550 555 560His Asp Leu Val Ile Thr Ile Glu Asp Gly Val Ile His Gly Gly Val
565 570 575Gly Ser Leu Leu Ser Asp Ala Leu Asn Ala Ser Glu Val Asp Thr Pro
580 585 590Arg Arg Gln Ile Ala Val Pro Gln Lys Tyr Leu Asp His Ala Ser Arg
595 600 605Asn Glu Val Leu Ala Asp Tyr Gly Leu Asp Ala Asp Gly Ile Glu Thr
610 615 620Thr Val Val Gly Trp Leu Asp Ser Leu Phe Gly Glu625 630 635<210>405<211>1061<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1038)<223>FRXA01562<400>405gag cag gat ttg gac gaa ttg atg cac tcc acg ggc gtc atc gat ccg 48Glu Gln Asp Leu Asp Glu Leu Met His Ser Thr Gly Val Ile Asp Pro1 5 10 15ctc aca gga gct cct aaa tct gca tca aag ccc ggt tgg acc tct gtg 96Leu Thr Gly Ala Pro Lys Ser Ala Ser Lys Pro Gly Trp Thr Ser Val
20 25 30ttc agc gat gag ctg gtc aag att ggt gcg cag aat gaa aac gtt gtt 144Phe Ser Asp Glu Leu Val Lys Ile Gly Ala Gln Asn Glu Asn Val Val
35 40 45gcc atc acc gcc gcg atg gca ggt cct acc ggt ctg tcc aag ttc gaa 192Ala Ile Thr Ala Ala Met Ala Gly Pro Thr Gly Leu Ser Lys Phe Glu
50 55 60gcc aat ttc ccc aac cga ttc ttt gat gtc ggc att gct gag cag cac 240Ala Asn Phe Pro Asn Arg Phe Phe Asp Val Gly Ile Ala Glu Gln His65 70 75 80gcg gta act tct gcc gca ggc ctc gca ttg ggt gga aaa cac cct gtg 288Ala Val Thr Ser Ala Ala Gly Leu Ala Leu Gly Gly Lys His Pro Val
85 90 95gtg gct att tac tcc acg ttc ttg aac cgc gct ttt gat cag ctg ctc 336Val Ala Ile Tyr Ser Thr Phe Leu Asn Arg Ala Phe Asp Gln Leu Leu
100 105 110atg gat gtg ggc atg ctc aac cag cct gtt act ttg gtg ctt gat cgc 384Met Asp Val Gly Met Leu Asn Gln Pro Val Thr Leu Val Leu Asp Arg
115 120 125tca ggt gtc acg ggt tcg gat gga gcg agc cac aat ggc gtc tgg gat 432Ser Gly Val Thr Gly Ser Asp Gly Ala Ser His Asn Gly Val Trp Asp
130 135 140atg gcg ctg acc tcg atc gtt cca ggc gtg cag gtg gcg gca cca cgt 480Met Ala Leu Thr Ser Ile Val Pro Gly Val Gln Val Ala Ala Pro Arg145 150 155 160gat gag gat tcc ttg cgt gag ctg ctc aat gag gct att tcc atc gat 528Asp Glu Asp Ser Leu Arg Glu Leu Leu Asn Glu Ala Ile Ser Ile Asp
165 170 175gat ggc ccc aca gtt gtg cgt ttc ccc aag ggc gac ttg cca act cca 576Asp Gly Pro Thr Val Val Arg Phe Pro Lys Gly Asp Leu Pro Thr Pro
180 185 190att gtt gct atc gac acc ttg gaa gac ggc gtg gat gtc ctc gca tat 624Ile Val Ala Ile Asp Thr Leu Glu Asp Gly Val Asp Val Leu Ala Tyr
195 200 205gaa gac gcc act gac gtt gaa tca acc gac gat gcg cca tca gtt ctc 672Glu Asp Ala Thr Asp Val Glu Ser Thr Asp Asp Ala Pro Ser Val Leu
210 215 220atc att gcg gta ggc gag cgc gca act gtt gca ctt gac gtt gct tcc 720Ile Ile Ala Val Gly Glu Arg Ala Thr Val Ala Leu Asp Val Ala Ser225 230 235 240agg att aaa cag cac ggc gtg aac gtc acg gtt gtt gac ccc cgc tgg 768Arg Ile Lys Gln His Gly Val Asn Val Thr Val Val Asp Pro Arg Trp
245 250 255att gtc ccc atc ccg cag tcc ttg gtc gcg ctg tct gat gat cat gac 816Ile Val Pro Ile Pro Gln Ser Leu Val Ala Leu Ser Asp Asp His Asp
260 265 270ctc gtg atc acc atc gaa gac ggc gtc atc cac ggc ggc gtg gga tcc 864Leu Val Ile Thr Ile Glu Asp Gly Val Ile His Gly Gly Val Gly Ser
275 280 285ttg ctc tct gat gcg ctt aac gcc tct gag gtg gat acc cct cgc cga 912Leu Leu Ser Asp Ala Leu Asn Ala Ser Glu Val Asp Thr Pro Arg Arg
290 295 300caa atc gcc gtg ccc cag aag tac ctg gat cac gcg tcc cgc aat gaa 960Gln Ile Ala Val Pro Gln Lys Tyr Leu Asp His Ala Ser Arg Asn Glu305 310 315 320gtg ctc gcc gat tat ggc ctc gac gcc gac ggc att gaa acc act gtt 1008Val Leu Ala Asp Tyr Gly Leu Asp Ala Asp Gly Ile Glu Thr Thr Val
325 330 335gtt gga tgg ctg gat tcc ctg ttc ggg gaa taaaaccctg cttatcgacg 1058Val Gly Trp Leu Asp Ser Leu Phe Gly Glu
340 345ccg 1061<210>406<211>346<212>PRT<213>谷氨酸棒杆菌<400>406Glu Gln Asp Leu Asp Glu Leu Met His Ser Thr Gly Val Ile Asp Pro1 5 10 15Leu Thr Gly Ala Pro Lys Ser Ala Ser Lys Pro Gly Trp Thr Ser Val
20 25 30Phe Ser Asp Glu Leu Val Lys Ile Gly Ala Gln Asn Glu Asn Val Val
35 40 45Ala Ile Thr Ala Ala Met Ala Gly Pro Thr Gly Leu Ser Lys Phe Glu
50 55 60Ala Asn Phe Pro Asn Arg Phe Phe Asp Val Gly Ile Ala Glu Gln His65 70 75 80Ala Val Thr Ser Ala Ala Gly Leu Ala Leu Gly Gly Lys His Pro Val
85 90 95Val Ala Ile Tyr Ser Thr Phe Leu Asn Arg Ala Phe Asp Gln Leu Leu
100 105 110Met Asp Val Gly Met Leu Asn Gln Pro Val Thr Leu Val Leu Asp Arg
115 120 125Ser Gly Val Thr Gly Ser Asp Gly Ala Ser His Asn Gly Val Trp Asp
130 135 140Met Ala Leu Thr Ser Ile Val Pro Gly Val Gln Val Ala Ala Pro Arg145 150 155 160Asp Glu Asp Ser Leu Arg Glu Leu Leu Asn Glu Ala Ile Ser Ile Asp
165 170 175Asp Gly Pro Thr Val Val Arg Phe Pro Lys Gly Asp Leu Pro Thr Pro
180 185 190Ile Val Ala Ile Asp Thr Leu Glu Asp Gly Val Asp Val Leu Ala Tyr
195 200 205Glu Asp Ala Thr Asp Val Glu Ser Thr Asp Asp Ala Pro Ser Val Leu
210 215 220Ile Ile Ala Val Gly Glu Arg Ala Thr Val Ala Leu Asp Val Ala Ser225 230 235 240Arg Ile Lys Gln His Gly Val Asn Val Thr Val Val Asp Pro Arg Trp
245 250 255Ile Val Pro Ile Pro Gln Ser Leu Val Ala Leu Ser Asp Asp His Asp
260 265 270Leu Val Ile Thr Ile Glu Asp Gly Val Ile His Gly Gly Val Gly Ser
275 280 285Leu Leu Ser Asp Ala Leu Asn Ala Ser Glu Val Asp Thr Pro Arg Arg
290 295 300Gln Ile Ala Val Pro Gln Lys Tyr Leu Asp His Ala Ser Arg Asn Glu305 310 315 320Val Leu Ala Asp Tyr Gly Leu Asp Ala Asp Gly Ile Glu Thr Thr Val
325 330 335Val Gly Trp Leu Asp Ser Leu Phe Gly Glu
340 345<210>407<211>703<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(703)<223>FRXA01705<400>407gcaggtgcac atttgttttg tcacctgcac aaaagtgtcg ccagcccgat acttgtacaa 60ccgtccgcat ccgagaagca aaggtgtctg actcgcgcca atg gga att ctg aac 115
Met Gly Ile Leu Asn
1 5agt att tca aca cct gct gac tta aag gcc ctt aat gat gag gat ttg 163Ser Ile Ser Thr Pro Ala Asp Leu Lys Ala Leu Asn Asp Glu Asp Leu
10 15 20gac gct ctt gcc aaa gaa atc cga act ttc ctg gtc gat aaa gtc gca 211Asp Ala Leu Ala Lys Glu Ile Arg Thr Phe Leu Val Asp Lys Val Ala
25 30 35gca act ggt ggc cac tta ggt cca aat ttg ggc gta gtg gaa tta acc 259Ala Thr Gly Gly His Leu Gly Pro Asn Leu Gly Val Val Glu Leu Thr
40 45 50atc ggt ctt cat cga gtt ttc gat tcg cct caa gac ccg atc atc ttt 307Ile Gly Leu His Arg Val Phe Asp Ser Pro Gln Asp Pro Ile Ile Phe
55 60 65gat act tct cac cag tcc tat gtg cat aag atc ctg acg ggt cgc gct 355Asp Thr Ser His Gln Ser Tyr Val His Lys Ile Leu Thr Gly Arg Ala70 75 80 85aaa gat ttt gat tct ttg cgt caa aaa gat ggc ctt tct ggt tac acc 403Lys Asp Phe Asp Ser Leu Arg Gln Lys Asp Gly Leu Ser Gly Tyr Thr
90 95 100tgc cgt gct gaa agt gag cac gat tgg act gag tct tcg cat gct tcg 451Cys Arg Ala Glu Ser Glu His Asp Trp Thr Glu Ser Ser His Ala Ser
105 110 115gcg gcc ttg tct tat gcg gat ggt ttg tct aaa gcc aag cag ttg gat 499Ala Ala Leu Ser Tyr Ala Asp Gly Leu Ser Lys Ala Lys Gln Leu Asp
120 125 130ggc gat acc acg cat agt gtg gtt gct gtc gtt ggt gat ggc gct cta 547Gly Asp Thr Thr His Ser Val Val Ala Val Val Gly Asp Gly Ala Leu
135 140 145act ggc ggc atg tgt tgg gaa gca ctg aac aat att gct gct ggt aaa 595Thr Gly Gly Met Cys Trp Glu Ala Leu Asn Asn Ile Ala Ala Gly Lys150 155 160 165gac cgc aaa gtt gtt gtc gta gtc aat gac aat ggc cgg agt tat tct 643Asp Arg Lys Val Val Val Val Val Asn Asp Asn Gly Arg Ser Tyr Ser
170 175 180cca acc att ggc gga ttt gcg gaa aac ctt gcg ggc ctt cgc atg cag 691Pro Thr Ile Gly Gly Phe Ala Glu Asn Leu Ala Gly Leu Arg Met Gln
185 190 195cct ttc tat gat 703Pro Phe Tyr Asp
200<210>408<211>201<212>PRT<213>谷氨酸棒杆菌<400>408Met Gly Ile Leu Asn Ser Ile Ser Thr Pro Ala Asp Leu Lys Ala Leu1 5 10 15Asn Asp Glu Asp Leu Asp Ala Leu Ala Lys Glu Ile Arg Thr Phe Leu
20 25 30Val Asp Lys Val Ala Ala Thr Gly Gly His Leu Gly Pro Asn Leu Gly
35 40 45Val Val Glu Leu Thr Ile Gly Leu His Arg Val Phe Asp Ser Pro Gln
50 55 60Asp Pro Ile Ile Phe Asp Thr Ser His Gln Ser Tyr Val His Lys Ile65 70 75 80Leu Thr Gly Arg Ala Lys Asp Phe Asp Ser Leu Arg Gln Lys Asp Gly
85 90 95Leu Ser Gly Tyr Thr Cys Arg Ala Glu Ser Glu His Asp Trp Thr Glu
100 105 110Ser Ser His Ala Ser Ala Ala Leu Ser Tyr Ala Asp Gly Leu Ser Lys
115 120 125Ala Lys Gln Leu Asp Gly Asp Thr Thr His Ser Val Val Ala Val Val
130 135 140Gly Asp Gly Ala Leu Thr Gly Gly Met Cys Trp Glu Ala Leu Asn Asn145 150 155 160Ile Ala Ala Gly Lys Asp Arg Lys Val Val Val Val Val Asn Asp Asn
165 170 175Gly Arg Ser Tyr Ser Pro Thr Ile Gly Gly Phe Ala Glu Asn Leu Ala
180 185 190Gly Leu Arg Met Gln Pro Phe Tyr Asp
195 200<210>409<211>2241<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2218)<223>RXN00879<400>409caccccaaag cccaatccaa aagatgtatt ttctaacaaa cttaccctca cgctacaaat 60atgctgtgcc cacacgctat tagtggcata atgttgtgtt gtg act gct cgc aga 115
Val Thr Ala Arg Arg
1 5ttt ttg aat gaa ctc gcc gat ctc tac ggc gta gca act tcc tac act 163Phe Leu Asn Glu Leu Ala Asp Leu Tyr Gly Val Ala Thr Ser Tyr Thr
10 15 20gat tac aaa ggt gcc cat att gag gtc agc gat gac aca tta gtg aaa 211Asp Tyr Lys Gly Ala His Ile Glu Val Ser Asp Asp Thr Leu Val Lys
25 30 35atc ctg cgt gct ctg ggt gtg aat tta gat aca agc aac ctc ccc aac 259Ile Leu Arg Ala Leu Gly Val Asn Leu Asp Thr Ser Asn Leu Pro Asn
40 45 50gat gac gct atc caa cgc caa att gcc ctc ttc cat gat cga gag ttc 307Asp Asp Ala Ile Gln Arg Gln Ile Ala Leu Phe His Asp Arg Glu Phe
55 60 65act cgc cca ctg cct cca tcg gtg gtt gca gtt gaa ggt gat gaa cta 355Thr Arg Pro Leu Pro Pro Ser Val Val Ala Val Glu Gly Asp Glu Leu70 75 80 85gtt ttc ccg gtg cat gtg cac gac ggt tcc cct gca gat gtc cac atc 403Val Phe Pro Val His Val His Asp Gly Ser Pro Ala Asp Val His Ile
90 95 100gaa ttg gaa gac ggc acg cag cgg gat gtt tct cag gtg gaa aac tgg 451Glu Leu Glu Asp Gly Thr Gln Arg Asp Val Ser Gln Val Glu Asn Trp
105 110 115aca gcg cca cgg gaa att gat ggg att agg tgg ggc gag gca tcg ttt 499Thr Ala Pro Arg Glu Ile Asp Gly Ile Arg Trp Gly Glu Ala Ser Phe
120 125 130aag att cct ggt gat ctc ccc ttg ggt tgg cac aag ctt cac ctt aaa 547Lys Ile Pro Gly Asp Leu Pro Leu Gly Trp His Lys Leu His Leu Lys
135 140 145tcc aat gaa cgc tca gct gag tgc ggt ttg atc atc acc ccg gct cgt 595Ser Asn Glu Arg Ser Ala Glu Cys Gly Leu Ile Ile Thr Pro Ala Arg150 155 160 165ctg tct act gct gat aag tat ctt gat tcc cct cgc agt ggt gtc atg 643Leu Ser Thr Ala Asp Lys Tyr Leu Asp Ser Pro Arg Ser Gly Val Met
170 175 180gcg cag atc tac tct gtg cgt tcc acg ttg tcg tgg ggc atg ggt gat 691Ala Gln Ile Tyr Ser Val Arg Ser Thr Leu Ser Trp Gly Met Gly Asp
185 190 195ttc aat gat tta gga aac ttg gca agt gtg gtt gcc cag gat gga gca 739Phe Asn Asp Leu Gly Asn Leu Ala Ser Val Val Ala Gln Asp Gly Ala
200 205 210gac ttc ctg ctc atc aac ccc atg cac gct gca gag ccg ctg cct cct 787Asp Phe Leu Leu Ile Asn Pro Met His Ala Ala Glu Pro Leu Pro Pro
215 220 225act gag gac tct cct tat ctg ccc aca acc agg cgc ttt atc aac ccg 835Thr Glu Asp Ser Pro Tyr Leu Pro Thr Thr Arg Arg Phe Ile Asn Pro230 235 240 245atc tac att cgg gga gaa gat att ccg gag ttt aat cag ctt gag att 883Ile Tyr Ile Arg Val Glu Asp Ile Pro Glu Phe Asn Gln Leu Glu Ile
250 255 260gat cta cgc gat gat atc gca gag atg gct gcg gaa ttc cgc gaa cgc 931Asp Leu Arg Asp Asp Ile Ala Glu Met Ala Ala Glu Phe Arg Glu Arg
265 270 275aat ctg acc tca gac atc att gag cgc aat gac gtc tac gct gca aag 979Asn Leu Thr Ser Asp Ile Ile Glu Arg Asn Asp Val Tyr Ala Ala Lys
280 285 290ctt caa gtg ctg cgc gcc att ttt gaa atg cct cgt tcc agc gaa cgt 1027Leu Gln Val Leu Arg Ala Ile Phe Glu Met Pro Arg Ser Ser Glu Arg
295 300 305gaa gcc aac ttt gtc tcc ttc gtg caa cgg gaa ggc caa ggt ctt att 1075Glu Ala Asn Phe Val Ser Phe Val Gln Arg Glu Gly Gln Gly Leu Ile310 315 320 325gat ttc gcc acc tgg tgc gcg gac cgc gaa act gca cag tct gaa tct 1123Asp Phe Ala Thr Trp Cys Ala Asp Arg Glu Thr Ala Gln Ser Glu Ser
330 335 340gtc cac gga act gag cca gac cgc gat gag ctg acc atg ttc tac atg 1171Val His Gly Thr Glu Pro Asp Arg Asp Glu Leu Thr Met Phe Tyr Met
345 350 355tgg ttg cag tgg cta tgt gat gag cag ctg gcg gca gct caa aag cgc 1219Trp Leu Gln Trp Leu Cys Asp Glu Gln Leu Ala Ala Ala Gln Lys Arg
360 365 370gct gtc gat gcc gga atg tcg atc ggc atc atg gca gac ctg gca gtt 1267Ala Val Asp Ala Gly Met Ser Ile Gly Ile Met Ala Asp Leu Ala Val
375 380 385ggt gtg cat cca ggt ggt gct gat gcc cag aac ctc agc cac gta ctt 1315Gly Val His Pro Gly Gly Ala Asp Ala Gln Asn Leu Ser His Val Leu390 395 400 405gct ccg gat gcg tca gtg ggc gcc cca cca gat gga tac aac cag cag 1363Ala Pro Asp Ala Ser Val Gly Ala Pro Pro Asp Gly Tyr Asn Gln Gln
410 415 420ggc caa gac tgg tcc cag cca cca tgg cat cca gtg cgt ctt gca gag 1411Gly Gln Asp Trp Ser Gln Pro Pro Trp His Pro Val Arg Leu Ala Glu
425 430 435gaa ggc tac att ccg tgg cgt aat ctg ctg cgc act gtg ctg cgt cac 1459Glu Gly Tyr Ile Pro Trp Arg Asn Leu Leu Arg Thr Val Leu Arg His
440 445 450tcc ggc gga atc cgc gtg gac cac gtt ctt ggt ttg ttc agg crc ttt 1507Ser Gly Gly Ile Arg Val Asp His Val Leu Gly Leu Phe Arg Leu Phe
455 460 465gtc atg cca cgc atg caa tcc cct gct acg ggc acc tat atc cgc ttc 1555Val Met Pro Arg Met Gln Ser Pro Ala Thr Gly Thr Tyr Ile Arg Phe470 475 480 485gac cat aat gcg ttg gta ggc att cta gcc cta gaa gca gaa ctc gca 1603Asp His Asn Ala Leu Val Gly Ile Leu Ala Leu Glu Ala Glu Leu Ala
490 495 500ggc gcc gtt gtc att ggt gaa gat ctg gga acg ttt gag cct tgg gta 1651Gly Ala Val Val Ile Gly Glu Asp Leu Gly Thr Phe Glu Pro Trp Val
505 510 515caa gat gca ttg gct cag cgt ggc atc atg ggc acc tcg atc cta tgg 1699Gln Asp Ala Leu Ala Gln Arg Gly Ile Met Gly Thr Ser Ile Leu Trp
520 525 530ttc gag cat tcc cca agc cag ccg ggt cct cgc cgc cag gaa gag tat 1747Phe Glu His Ser Pro Ser Gln Pro Gly Pro Arg Arg Gln Glu Glu Tyr
535 540 545cgt ccg ctg gcc ttg acc act gtg acc act cat gat ctc cct ccg act 1795Arg Pro Leu Ala Leu Thr Thr Val Thr Thr His Asp Leu Pro Pro Thr550 555 560 565gct ggt tat ttg gag ggc gag cac att gct ctt cgt gag cga ttg ggg 1843Ala Gly Tyr Leu Glu Gly Glu His Ile Ala Leu Arg Glu Arg Leu Gly
570 575 580gtg ctc aac act gat cct gct gca gaa ctc gct gag gat ctg cag tgg 1891Val Leu Asn Thr Asp Pro Ala Ala Glu Leu Ala Glu Asp Leu Gln Trp
585 590 595caa gcg gag atc ctt gat gtc gca gca tct gcc aac gca ttg cca gcc 1939Gln Ala Glu Ile Leu Asp Val Ala Ala Ser Ala Asn Ala Leu Pro Ala
600 605 610cgg gaa tac gtg gga ctc gaa cgc gat cag cgc ggt gag ttg gct gag 1987Arg Glu Tyr Val Gly Leu Glu Arg Asp Gln Arg Gly Glu Leu Ala Glu
615 620 625ctg ttg gaa ggc ctg cac act ttc gtt gcg aaa acc cct tca gca ctg 2035Leu Leu Glu Gly Leu His Thr Phe Val Ala Lys Thr Pro Ser Ala Leu630 635 640 645acc tgt gtc tgc ttg gta gac atg gtc ggt gaa aag cgg gca cag aat 2083Thr Cys Val Cys Leu Val Asp Met Val Gly Glu Lys Arg Ala Gln Asn
650 655 660cag ccg ggc aca acg agg gat atg tat ccc aac tgg tgt atc cca ctg 2131Gln Pro Gly Thr Thr Arg Asp Met Tyr Pro Asn Trp Cys Ile Pro Leu
665 670 675tgt gac agc gaa ggc aac tcc gtg ctc att gaa tcg ctg cgt gaa aat 2179Cys Asp Ser Glu Gly Asn Ser Val Leu Ile Glu Ser Leu Arg Glu Asn
680 685 690gag ctg tat cac cgt gtg gca aag gca agc aag cga gat taggtccgct 2228Glu Leu Tyr His Arg Val Ala Lys Ala Ser Lys Arg Asp
695 700 705tcagttgtgg tgg 2241<210>410<211>706<212>PRT<213>谷氨酸棒杆菌<400>410Val Thr Ala Arg Arg Phe Leu Asn Glu Leu Ala Asp Leu Tyr Gly Val1 5 10 15Ala Thr Ser Tyr Thr Asp Tyr Lys Gly Ala His Ile Glu Val Ser Asp
20 25 30Asp Thr Leu Val Lys Ile Leu Arg Ala Leu Gly Val Asn Leu Asp Thr
35 40 45Ser Asn Leu Pro Asn Asp Asp Ala Ile Gln Arg Gln Ile Ala Leu Phe
50 55 60His Asp Arg Glu Phe Thr Arg Pro Leu Pro Pro Ser Val Val Ala Val65 70 75 80Glu Gly Asp Glu Leu Val Phe Pro Val His Val His Asp Gly Ser Pro
85 90 95Ala Asp Val His Ile Glu Leu Glu Asp Gly Thr Gln Arg Asp Val Ser
100 105 110Gln Val Glu Asn Trp Thr Ala Pro Arg Glu Ile Asp Gly Ile Arg Trp
115 120 125Gly Glu Ala Ser Phe Lys Ile Pro Gly Asp Leu Pro Leu Gly Trp His
130 135 140Lys Leu His Leu Lys Ser Asn Glu Arg Ser Ala Glu Cys Gly Leu Ile145 150 155 160Ile Thr Pro Ala Arg Leu Ser Thr Ala Asp Lys Tyr Leu Asp Ser Pro
165 170 175Arg Ser Gly Val Met Ala Gln Ile Tyr Ser Val Arg Ser Thr Leu Ser
180 185 190Trp Gly Met Gly Asp Phe Asn Asp Leu Gly Asn Leu Ala Ser Val Val
195 200 205Ala Gln Asp Gly Ala Asp Phe Leu Leu Ile Asn Pro Met His Ala Ala
210 215 220Glu Pro Leu Pro Pro Thr Glu Asp Ser Pro Tyr Leu Pro Thr Thr Arg225 230 235 240Arg Phe Ile Asn Pro Ile Tyr Ile Arg Val Glu Asp Ile Pro Glu Phe
245 250 255Asn Gln Leu Glu Ile Asp Leu Arg Asp Asp Ile Ala Glu Met Ala Ala
260 265 270Glu Phe Arg Glu Arg Asn Leu Thr Ser Asp Ile Ile Glu Arg Asn Asp
275 280 285Val Tyr Ala Ala Lys Leu Gln Val Leu Arg Ala Ile Phe Glu Met Pro
290 295 300Arg Ser Ser Glu Arg Glu Ala Asn Phe Val Ser Phe Val Gln Arg Glu305 310 315 320Gly Gln Gly Leu Ile Asp Phe Ala Thr Trp Cys Ala Asp Arg Glu Thr
325 330 335Ala Gln Ser Glu Ser Val His Gly Thr Glu Pro Asp Arg Asp Glu Leu
340 345 350Thr Met Phe Tyr Met Trp Leu Gln Trp Leu Cys Asp Glu Gln Leu Ala
355 360 365Ala Ala Gln Lys Arg Ala Val Asp Ala Gly Met Ser Ile Gly Ile Met
370 375 380Ala Asp Leu Ala Val Gly Val His Pro Gly Gly Ala Asp Ala Gln Asn385 390 395 400Leu Ser His Val Leu Ala Pro Asp Ala Ser Val Gly Ala Pro Pro Asp
405 410 415Gly Tyr Asn Gln Gln Gly Gln Asp Trp Ser Gln Pro Pro Trp His Pro
420 425 430Val Arg Leu Ala Glu Glu Gly Tyr Ile Pro Trp Arg Asn Leu Leu Arg
435 440 445Thr Val Leu Arg His Ser Gly Gly Ile Arg Val Asp His Val Leu Gly
450 455 460Leu Phe Arg Leu Phe Val Met Pro Arg Met Gln Ser Pro Ala Thr Gly465 470 475 480Thr Tyr Ile Arg Phe Asp His Asn Ala Leu Val Gly Ile Leu Ala Leu
485 490 495Glu Ala Glu Leu Ala Gly Ala Val Val Ile Gly Glu Asp Leu Gly Thr
500 505 510Phe Glu Pro Trp Val Gln Asp Ala Leu Ala Gln Arg Gly Ile Met Gly
515 520 525Thr Ser Ile Leu Trp Phe Glu His Ser Pro Ser Gln Pro Gly Pro Arg
530 535 540Arg Gln Glu Glu Tyr Arg Pro Leu Ala Leu Thr Thr Val Thr Thr His545 550 555 560Asp Leu Pro Pro Thr Ala Gly Tyr Leu Glu Gly Glu His Ile Ala Leu
565 570 575Arg Glu Arg Leu Gly Val Leu Asn Thr Asp Pro Ala Ala Glu Leu Ala
580 585 590Glu Asp Leu Gln Trp Gln Ala Glu Ile Leu Asp Val Ala Ala Ser Ala
595 600 605Asn Ala Leu Pro Ala Arg Glu Tyr Val Gly Leu Glu Arg Asp Gln Arg
610 615 620Gly Glu Leu Ala Glu Leu Leu Glu Gly Leu His Thr Phe Val Ala Lys625 630 635 640Thr Pro Ser Ala Leu Thr Cys Val Cys Leu Val Asp Met Val Gly Glu
645 650 655Lys Arg Ala Gln Asn Gln Pro Gly Thr Thr Arg Asp Met Tyr Pro Asn
660 665 670Trp Cys Ile Pro Leu Cys Asp Ser Glu Gly Asn Ser Val Leu Ile Glu
675 680 685Ser Leu Arg Glu Asn Glu Leu Tyr His Arg Val Ala Lys Ala Ser Lys
690 695 700Arg Asp705<210>411<211>2223<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2200)<223>FRXA00879<400>411aaaagatgta ttttctaaca aacttaccct cacgctacaa atatgctgtg cccacacgct 60attagtggca taatgttgtg ttgtgactgc tcgcagattt ttg aat gaa ctc gcc 115
Leu Asn Glu Leu Ala
1 5gat ctc tac ggc gta gca act tcc tac act gat tac aaa ggt gcc cat 163Asp Leu Tyr Gly Val Ala Thr Ser Tyr Thr Asp Tyr Lys Gly Ala His
10 15 20att gag gtc agc gat gac aca tta gtg aaa atc ctg cgt gct ctg ggt 211Ile Glu Val Ser Asp Asp Thr Leu Val Lys Ile Leu Arg Ala Leu Gly
25 30 35gtg aat tta gat aca agc aac ctc ccc aac gat gac gct atc caa cgc 259Val Asn Leu Asp Thr Ser Asn Leu Pro Asn Asp Asp Ala Ile Gln Arg
40 45 50caa att gcc ctc ttc cat gat cga gag ttc act cgc cca ctg cct cca 307Gln Ile Ala Leu Phe His Asp Arg Glu Phe Thr Arg Pro Leu Pro Pro
55 60 65tcg gtg gtt gca gtt gaa ggt gat gaa cta gtt ttg ccg gtg cat gtg 355Ser Val Val Ala Val Glu Gly Asp Glu Leu Val Phe Pro Val His Val70 75 80 85cac gac ggt tcc cct gca gat gtc cac atc gaa ttg gaa gac ggc acg 403His Asp Gly Ser Pro Ala Asp Val His Ile Glu Leu Glu Asp Gly Thr
90 95 100cag cgg gat gtt tct cag gtg gaa aac tgg aca gcg cca cgg gaa att 451Gln Arg Asp Val Ser Gln Val Glu Asn Trp Thr Ala Pro Arg Glu Ile
105 110 115gat ggg att agg tgg ggc gag gca tcg ttt aag att cct ggt gat ctc 499Asp Gly Ile Arg Trp Gly Glu Ala Ser Phe Lys Ile Pro Gly Asp Leu
120 125 130ccc ttg ggt tgg cac aag ctt cac ctt aaa tcc aat gaa cgc tca gct 547Pro Leu Gly Trp His Lys Leu His Leu Lys Ser Asn Glu Arg Ser Ala
135 140 145gag tgc ggt ttg atc atc acc ccg gct cgt ctg tct act gct gat aag 595Glu Cys Gly Leu Ile Ile Thr Pro Ala Arg Leu Ser Thr Ala Asp Lys150 155 160 165tat ctt gat tcc cct cgc agt ggt gtc atg gcg cag atc tac tct gtg 643Tyr Leu Asp Ser Pro Arg Ser Gly Val Met Ala Gln Ile Tyr Ser Val
170 175 180cgt tcc acg ttg tcg tgg ggc atg ggt gat ttc aat gat tta gga aac 691Arg Ser Thr Leu Ser Trp Gly Met Gly Asp Phe Asn Asp Leu Gly Asn
185 190 195ttg gca agt gtg gtt gcc cag gat gga gca gac ttc ctg ctc atc aac 739Leu Ala Ser Val Val Ala Gln Asp Gly Ala Asp Phe Leu Leu Ile Asn
200 205 210ccc atg cac gct gca gag ccg ctg cct cct act gag gac tct cct tat 787Pro Met His Ala Ala Glu Pro Leu Pro Pro Thr Glu Asp Ser Pro Tyr
215 220 225ctg ccc aca acc agg cgc ttt atc aac ccg atc tac att cgg gta gaa 835Leu Pro Thr Thr Arg Arg Phe Ile Asn Pro Ile Tyr Ile Arg Val Glu230 235 240 245gat att ccg gag ttt aat cag ctt gag att gat cta cgc gat gat atc 883Asp Ile Pro Glu Phe Asn Gln Leu Glu Ile Asp Leu Arg Asp Asp Ile
250 255 260gca gag atg gct gcg gaa ttc cgc gaa cgc aat ctg acc tca gac atc 931Ala Glu Met Ala Ala Glu Phe Arg Glu Arg Asn Leu Thr Ser Asp Ile
265 270 275att gag cgc aat gac gtc tac gct gca aag ctt caa gtg ctg cgc gcc 979Ile Glu Arg Asn Asp Val Tyr Ala Ala Lys Leu Gln Val Leu Arg Ala
280 285 290att ttt gaa atg cct cgt tcc agc gaa cgt gaa gcc aac ttt gtc tcc 1027Ile Phe Glu Met Pro Arg Ser Ser Glu Arg Glu Ala Asn Phe Val Ser
295 300 305ttc gtg caa cgg gaa ggc caa ggt ctt att gat ttc gcc acc tgg tgc 1075Phe Val Gln Arg Glu Gly Gln Gly Leu Ile Asp Phe Ala Thr Trp Cys310 315 320 325gcg gac cgc gaa act gca cag tct gaa tct gtc cac gga act gag cca 1123Ala Asp Arg Glu Thr Ala Gln Ser Glu Ser Val His Gly Thr Glu Pro
330 335 340gac cgc gat gag ctg acc atg ttc tac atg tgg ttg cag tgg cta tgt 1171Asp Arg Asp Glu Leu Thr Met Phe Tyr Met Trp Leu Gln Trp Leu Cys
345 350 355gat gag cag ctg gcg gca gct caa aag cgc gct gtc gat gcc gga atg 1219Asp Glu Gln Leu Ala Ala Ala Gln Lys Arg Ala Val Asp Ala Gly Met
360 365 370tcg atc ggc atc atg gca gac ctg gca gtt ggt gtg cat cca ggt ggt 1267Ser Ile Gly Ile Met Ala Asp Leu Ala Val Gly Val His Pro Gly Gly
375 380 385gct gat gcc cag aac ctc agc cac gta ctt gct ccg gat gcg tca gtg 1315Ala Asp Ala Gln Asn Leu Ser His Val Leu Ala Pro Asp Ala Ser Val390 395 400 405ggc gcc cca cca gat gga tac aac cag cag ggc caa gac tgg tcc cag 1363Gly Ala Pro Pro Asp Gly Tyr Asn Gln Gln Gly Gln Asp Trp Ser Gln
410 415 420cca cca tgg cat cca gtg cgt ctt gca gag gaa ggc tac att ccg tgg 1411Pro Pro Trp His Pro Val Arg Leu Ala Glu Glu Gly Tyr Ile Pro Trp
425 430 435cgt aat ctg ctg cgc act gtg ctg cgt cac tcc ggc gga atc cgc gtg 1459Arg Asn Leu Leu Arg Thr Val Leu Arg His Ser Gly Gly Ile Arg Val
440 445 450gac cac gtt ctt ggt ttg ttc agg ctc ttt gtc atg cca cgc atg caa 1507Asp His Val Leu Gly Leu Phe Arg Leu Phe Val Met Pro Arg Met Gln
455 460 465tcc cct gct acg ggc acc tat atc cgc ttc gac cat aat gcg ttg gta 1555Ser Pro Ala Thr Gly Thr Tyr Ile Arg Phe Asp His Asn Ala Leu Val470 475 480 485ggc att cta gcc cta gaa gca gaa ctc gca ggc gcc gtt gtc att ggt 1603Gly Ile Leu Ala Leu Glu Ala Glu Leu Ala Gly Ala Val Val Ile Gly
490 495 500gaa gat ctg gga acg ttt gag cct tgg gta caa gat gca ttg gct cag 1651Glu Asp Leu Gly Thr Phe Glu Pro Trp Val Gln Asp Ala Leu Ala Gln
505 510 515cgt ggc atc atg ggc acc tcg atc cta tgg ttc gag cat tcc cca agc 1699Arg Gly Ile Met Gly Thr Ser Ile Leu Trp Phe Glu His Ser Pro Ser
520 525 530cag ccg ggt cct cgc cgc cag gaa gag tat cgt ccg ctg gcc ttg acc 1747Gln Pro Gly Pro Arg Arg Gln Glu Glu Tyr Arg Pro Leu Ala Leu Thr
535 540 545act gtg acc act cat gat ctc cct ccg act gct ggt tat ttg gag ggc 1795Thr Val Thr Thr His Asp Leu Pro Pro Thr Ala Gly Tyr Leu Glu Gly550 555 560 565gag cac att gct ctt cgt gag cga ttg ggg gtg ctc aac act gat cct 1843Glu His Ile Ala Leu Arg Glu Arg Leu Gly Val Leu Asn Thr Asp Pro
570 575 580gct gca gaa ctc gct gag gat ctg cag tgg caa gcg gag atc ctt gat 1891Ala Ala Glu Leu Ala Glu Asp Leu Gln Trp Gln Ala Glu Ile Leu Asp
585 590 595gtc gca gca tct gcc aac gca ttg cca gcc cgg gaa tac gtg gga ctc 1939Val Ala Ala Set Ala Asn Ala Leu Pro Ala Arg Glu Tyr Val Gly Leu
600 605 610gaa cgc gat cag cgc ggt gag ttg gct gag ctg ttg gaa ggc ctg cac 1987Glu Arg Asp Gln Arg Gly Glu Leu Ala Glu Leu Leu Glu Gly Leu His
615 620 625act ttc gtt gcg aaa acc cct tca gca ctg acc tgt gtc tgc ttg gta 2035Thr Phe Val Ala Lys Thr Pro Ser Ala Leu Thr Cys Val Cys Leu Val630 635 640 645gac atg gtc ggt gaa aag cgg gca cag aat cag ccg ggc aca acg agg 2083Asp Met Val Gly Glu Lys Arg Ala Gln Asn Gln Pro Gly Thr Thr Arg
650 655 660gat atg tat ccc aac tgg tgt atc cca ctg tgt gac agc gaa ggc aac 2131Asp Met Tyr Pro Asn Trp Cys Ile Pro Leu Cys Asp Ser Glu Gly Asn
665 670 675tcc gtg ctc att gaa tcg ctg cgt gaa aat gag ctg tat cac cgt gtg 2179Ser Val Leu Ile Glu Ser Leu Arg Glu Asn Glu Leu Tyr His Arg Val
680 685 690gca aag gca agc aag cga gat taggtccgct tcagttgtgg tgg 2223Ala Lys Ala Ser Lys Arg Asp
695 700<210>412<211>700<212>PRT<213>谷氨酸棒杆菌<400>412Leu Asn Glu Leu Ala Asp Leu Tyr Gly Val Ala Thr Ser Tyr Thr Asp1 5 10 15Tyr Lys Gly Ala His Ile Glu Val Ser Asp Asp Thr Leu Val Lys Ile
20 25 30Leu Arg Ala Leu Gly Val Asn Leu Asp Thr Ser Asn Leu Pro Asn Asp
35 40 45Asp Ala Ile Gln Arg Gln Ile Ala Leu Phe His Asp Arg Glu Phe Thr
50 55 60Arg Pro Leu Pro Pro Ser Val Val Ala Val Glu Gly Asp Glu Leu Val65 70 75 80Phe Pro Val His Val His Asp Gly Ser Pro Ala Asp Val His Ile Glu
85 90 95Leu Glu Asp Gly Thr Gln Arg Asp Val Ser Gln Val Glu Asn Trp Thr
100 105 110Ala Pro Arg Glu Ile Asp Gly Ile Arg Trp Gly Glu Ala Ser Phe Lys
115 120 125Ile Pro Gly Asp Leu Pro Leu Gly Trp His Lys Leu His Leu Lys Ser
130 135 140Asn Glu Arg Ser Ala Glu Cys Gly Leu Ile Ile Thr Pro Ala Arg Leu145 150 155 160Ser Thr Ala Asp Lys Tyr Leu Asp Ser Pro Arg Ser Gly Val Met Ala
165 170 175Gln Ile Tyr Ser Val Arg Ser Thr Leu Ser Trp Gly Met Gly Asp Phe
180 185 190Asn Asp Leu Gly Asn Leu Ala Ser Val Val Ala Gln Asp Gly Ala Asp
195 200 205Phe Leu Leu Ile Asn Pro Met His Ala Ala Glu Pro Leu Pro Pro Thr
210 215 220Glu Asp Ser Pro Tyr Leu Pro Thr Thr Arg Arg Phe Ile Asn Pro Ile225 230 235 240Tyr Ile Arg Val Glu Asp Ile Pro Glu Phe Asn Gln Leu Glu Ile Asp
245 250 255Leu Arg Asp Asp Ile Ala Glu Met Ala Ala Glu Phe Arg Glu Arg Asn
260 265 270Leu Thr Ser Asp Ile Ile Glu Arg Asn Asp Val Tyr Ala Ala Lys Leu
275 280 285Gln Val Leu Arg Ala Ile Phe Glu Met Pro Arg Ser Ser Glu Arg Glu
290 295 300Ala Asn Phe Val Ser Phe Val Gln Arg Glu Gly Gln Gly Leu Ile Asp305 310 315 320Phe Ala Thr Trp Cys Ala Asp Arg Glu Thr Ala Gln Ser Glu Ser Val
325 330 335His Gly Thr Glu Pro Asp Arg Asp Glu Leu Thr Met Phe Tyr Met Trp
340 345 350Leu Gln Trp Leu Cys Asp Glu Gln Leu Ala Ala Ala Gln Lys Arg Ala
355 360 365Val Asp Ala Gly Met Ser Ile Gly Ile Met Ala Asp Leu Ala Val Gly
370 375 380Val His Pro Gly Gly Ala Asp Ala Gln Asn Leu Ser His Val Leu Ala385 390 395 400Pro Asp Ala Ser Val Gly Ala Pro Pro Asp Gly Tyr Asn Gln Gln Gly
405 410 415Gln Asp Trp Ser Gln Pro Pro Trp His Pro Val Arg Leu Ala Glu Glu
420 425 430Gly Tyr Ile Pro Trp Arg Asn Leu Leu Arg Thr Val Leu Arg His Ser
435 440 445Gly Gly Ile Arg Val Asp His Val Leu Gly Leu Phe Arg Leu Phe Val
450 455 460Met Pro Arg Met Gln Ser Pro Ala Thr Gly Thr Tyr Ile Arg Phe Asp465 470 475 480His Asn Ala Leu Val Gly Ile Leu Ala Leu Glu Ala Glu Leu Ala Gly
485 490 495Ala Val Val Ile Gly Glu Asp Leu Gly Thr Phe Glu Pro Trp Val Gln
500 505 510Asp Ala Leu Ala Gln Arg Gly Ile Met Gly Thr Ser Ile Leu Trp Phe
515 520 525Glu His Ser Pro Ser Gln Pro Gly Pro Arg Arg Gln Glu Glu Tyr Arg
530 535 540Pro Leu Ala Leu Thr Thr Val Thr Thr His Asp Leu Pro Pro Thr Ala545 550 555 560Gly Tyr Leu Glu Gly Glu His Ile Ala Leu Arg Glu Arg Leu Gly Val
565 570 575Leu Asn Thr Asp Pro Ala Ala Glu Leu Ala Glu Asp Leu Gln Trp Gln
580 585 590Ala Glu Ile Leu Asp Val Ala Ala Ser Ala Asn Ala Leu Pro Ala Arg
595 600 605Glu Tyr Val Gly Leu Glu Arg Asp Gln Arg Gly Glu Leu Ala Glu Leu
610 615 620Leu Glu Gly Leu His Thr Phe Val Ala Lys Thr Pro Ser Ala Leu Thr625 630 635 640Cys Val Cys Leu Val Asp Met Val Gly Glu Lys Arg Ala Gln Asn Gln
645 650 655Pro Gly Thr Thr Arg Asp Met Tyr Pro Asn Trp Cys Ile Pro Leu Cys
660 665 670Asp Ser Glu Gly Asn Ser Val Leu Ile Glu Ser Leu Arg Glu Asn Glu
675 680 685Leu Tyr His Arg Val Ala Lys Ala Ser Lys Arg Asp
690 695 700<210>413<211>1287<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1264)<223>RXN00043<400>413aacagcaggc ctcaagtccg aagataatta acctaaatcc gtagacataa gacatcatac 60gtcctatgct tgctggaagg aagcaaataa cctcagaaag atg gca gaa gtg gtg 115
Met Ala Glu Val Val
1 5cat tat caa gaa aat gca ggt caa gca gtt aaa aaa att gaa gaa aga 163His Tyr Gln Glu Asn Ala Gly Gln Ala Val Lys Lys Ile Glu Gly Arg
10 15 20att gtt acc ccc cac ggg gtg att gat ggc ttt ctc caa ctc gaa aac 211Ile Val Thr Pro His Gly Val Ile Asp Gly Phe Leu Gln Leu Glu Asn
25 30 35ggc atc atc acg gaa ctc tct gga gaa cca gca cct aaa aac gca gga 259Gly Ile Ile Thr Glu Leu Ser Gly Glu Pro Ala Pro Lys Asn Ala Gly
40 45 50ttc cac ccc gaa ctc ccc acg att gtt ccc agt ttt att gat ctt cat 307Phe His Pro Glu Leu Pro Thr Ile Val Pro Ser Phe Ile Asp Leu His
55 60 65aat cac ggt gga aac ggt ggc gcg ttt cct acg gga acg cag gac cag 355Asn His Gly Gly Asn Gly Gly Ala Phe Pro Thr Gly Thr Gln Asp Gln70 75 80 85gcg agg aat gcc gcg cag tat cac cgc gaa cat ggc acg acc gtg atg 403Ala Arg Asn Ala Ala Gln Tyr His Arg Glu His Gly Thr Thr Val Met
90 95 100ttg gca agc atg gtt tcg gcg ccg gct gac gca ctg gca gcg cag gtg 451Leu Ala Ser Met Val Ser Ala Pro Ala Asp Ala Leu Ala Ala Gln Val
105 110 115gaa aac ctt att ccc ttg tgt gaa gag ggc ctg ctg tgc ggc att cac 499Glu Asn Leu Ile Pro Leu Cys Glu Glu Gly Leu Leu Cys Gly Ile His
120 125 130ctc gag ggt cct ttc atc aac gca tgc cgt tgt ggt gct caa aac ccg 547Leu Glu Gly Pro Phe Ile Asn Ala Cys Arg Cys Gly Ala Gln Asn Pro
135 140 145gat ttt att ttt ccc ggc aac cca aca gat ctt gcc cag gtg atc cat 595Asp Phe Ile Phe Pro Gly Asn Pro Thr Asp Leu Ala Gln Val Ile His150 155 160 165gcg gga aaa ggt tgg atc aaa tcg atc aca gta gcg ccg gaa act gac 643Ala Gly Lys Gly Trp Ile Lys Ser Ile Thr Val Ala Pro Glu Thr Asp
170 175 180aat ctt act gag ctt ctc gat ctc tgc gca gcg cac cac atc att gct 691Asn Leu Thr Glu Leu Leu Asp Leu Cys Ala Ala His His Ile Ile Ala
185 190 195tcc ttc ggg cac act gat gca gat ttt gat acc act acc agc gca att 739Ser Phe Gly His Thr Asp Ala Asp Phe Asp Thr Thr Thr Ser Ala Ile
200 205 210gcc ttg gct aaa gag aaa aat gtg acg gtc acg gct acg cat ttg ttc 787Ala Leu Ala Lys Glu Lys Asn Val Thr Val Thr Ala Thr His Leu Phe
215 220 225aat gcg atg cct ccg ctg cat cat agg gat ccc ggc agc gtg ggc gct 835Asn Ala Met Pro Pro Leu His His Arg Asp Pro Gly Ser Val Gly Ala230 235 240 245ttg ctt gct gcg gca cgt gcc ggg gac gca tat gtt gag ttg atc gcc 883Leu Leu Ala Ala Ala Arg Ala Gly Asp Ala Tyr Val Glu Leu Ile Ala
250 255 260gac ggc gtg cat ttg gcc gat gga acg gtc gat cta gct cgt tcc aac 931Asp Gly Val His Leu Ala Asp Gly Thr Val Asp Leu Ala Arg Ser Asn
265 270 275aac gcc ttt ttc atc acg gac gcc atg gaa gcc gcc gga atg cca gac 979Asn Ala Phe Phe Ile Thr Asp Ala Met Glu Ala Ala Gly Met Pro Asp
280 285 290ggt gag tac att ttg ggc gtt ttg aac gtc acc gtc acc gat ggc gtc 1027Gly Glu Tyr Ile Leu Gly Val Leu Asn Val Thr Val Thr Asp Gly Val
295 300 305gcc cgt ctg cgc gat ggc ggc gcc atc gcc ggg ggt acc agc aca cta 1075Ala Arg Leu Arg Asp Gly Gly Ala Ile Ala Gly Gly Thr Ser Thr Leu310 315 320 325gcg agt cag ttc gtg cac cac gtg cgc agg ggt atg acg ctt atc gac 1123Ala Ser Gln Phe Val His His Val Arg Arg Gly Met Thr Leu Ile Asp
330 335 340gcg acc ctc cac acc tca acc gtc gcc gcc aaa att ctc gga ctt agc 1171Ala Thr Leu His Thr Ser Thr Val Ala Ala Lys Ile Leu Gly Leu Ser
345 350 355gat cac gaa atc gtt aaa tcc aac cct gta aat ttt gtg gtc ttt gac 1219Asp His Glu Ile Val Lys Ser Asn Pro Val Asn Phe Val Val Phe Asp
360 365 370tca aac ggc cag tta caa cag gtc cat tta gac cat caa gta att 1264Ser Asn Gly Gln Leu Gln Gln Val His Leu Asp His Gln Val Ile
375 380 385taaatacgag caaaactttc ctg 1287<210>414<211>388<212>PRT<213>谷氨酸棒杆菌<400> 414Met Ala Glu Val Val His Tyr Gln Glu Asn Ala Gly Gln Ala Val Lys1 5 10 15Lys Ile Glu Gly Arg Ile Val Thr Pro His Gly Val Ile Asp Gly Phe
20 25 30Leu Gln Leu Glu Asn Gly Ile Ile Thr Glu Leu Ser Gly Glu Pro Ala
35 40 45Pro Lys Asn Ala Gly Phe His Pro Glu Leu Pro Thr Ile Val Pro Ser
50 55 60Phe Ile Asp Leu His Asn His Gly Gly Asn Gly Gly Ala Phe Pro Thr65 70 75 80Gly Thr Gln Asp Gln Ala Arg Asn Ala Ala Gln Tyr His Arg Glu His
85 90 95Gly Thr Thr Val Met Leu Ala Ser Met Val Ser Ala Pro Ala Asp Ala
100 105 110Leu Ala Ala Gln Val Glu Asn Leu Ile Pro Leu Cys Glu Glu Gly Leu
115 120 125Leu Cys Gly Ile His Leu Glu Gly Pro Phe Ile Asn Ala Cys Arg Cys
130 135 140Gly Ala Gln Asn Pro Asp Phe Ile Phe Pro Gly Asn Pro Thr Asp Leu145 150 155 160Ala Gln Val Ile His Ala Gly Lys Gly Trp Ile Lys Ser Ile Thr Val
165 170 175Ala Pro Glu Thr Asp Asn Leu Thr Glu Leu Leu Asp Leu Cys Ala Ala
180 185 190His His Ile Ile Ala Ser Phe Gly His Thr Asp Ala Asp Phe Asp Thr
195 200 205Thr Thr Ser Ala Ile Ala Leu Ala Lys Glu Lys Asn Val Thr Val Thr
210 215 220Ala Thr His Leu Phe Asn Ala Met Pro Pro Leu His His Arg Asp Pro225 230 235 240Gly Ser Val Gly Ala Leu Leu Ala Ala Ala Arg Ala Gly Asp Ala Tyr
245 250 255Val Glu Leu Ile Ala Asp Gly Val His Leu Ala Asp Gly Thr Val Asp
260 265 270Leu Ala Arg Ser Asn Asn Ala Phe Phe Ile Thr Asp Ala Met Glu Ala
275 280 285Ala Gly Met Pro Asp Gly Glu Tyr Ile Leu Gly Val Leu Asn Val Thr
290 295 300Val Thr Asp Gly Val Ala Arg Leu Arg Asp Gly Gly Ala Ile Ala Gly305 310 315 320Gly Thr Ser Thr Leu Ala Ser Gln Phe Val His His Val Arg Arg Gly
325 330 335Met Thr Leu Ile Asp Ala Thr Leu His Thr Ser Thr Val Ala Ala Lys
340 345 350Ile Leu Gly Leu Ser Asp His Glu Ile Val Lys Ser Asn Pro Val Asn
355 360 365Phe Val Val Phe Asp Ser Asn Gly Gln Leu Gln Gln Val His Leu Asp
370 375 380His Gln Val Ile385<210>415<211>1287<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1264)<223>FRXA00043<400>415aacagcaggc ctcaagtccg aagataatta acctaaatcc gtagacataa gacatcatac 60gtcctatgct tgctggaagg aagcaaataa cctcagaaag atg gca gaa gtg gtg 115
Met Ala Glu Val Val
1 5cat tat caa gaa aat gca ggt caa gca gtt aaa aaa att gaa gga aga 163His Tyr Gln Glu Asn Ala Gly Gln Ala Val Lys Lys Ile Glu Gly Arg
10 15 20att gtt acc ccc cac ggg gtg att gat ggc ttt ctc caa ctc gaa aac 211Ile Val Thr Pro His Gly Val Ile Asp Gly Phe Leu Gln Leu Glu Asn
25 30 35ggc atc atc acg gaa ctc tct gga gaa cca gca cct aaa aac gca gga 259Gly Ile Ile Thr Glu Leu Ser Gly Glu Pro Ala Pro Lys Asn Ala Gly
40 45 50ttc cac ccc gaa ctc ccc acg att gtt ccc agt ttt att gat ctt cat 307Phe His Pro Glu Leu Pro Thr Ile Val Pro Ser Phe Ile Asp Leu His
55 60 65aat cac ggt gga aac ggt ggc gcg ttt cct acg gga acg cag gac cag 355Asn His Gly Gly Asn Gly Gly Ala Phe Pro Thr Gly Thr Gln Asp Gln70 75 80 85gcg agg aat gcc gcg cag tat cac cgc gaa cat ggc acg acc gtg atg 403Ala Arg Asn Ala Ala Gln Tyr His Arg Glu His Gly Thr Thr Val Met
90 95 100ttg gca agc atg gtt tcg gcg ccg gct gac gca ctg gca gcg cag gtg 451Leu Ala Ser Met Val Ser Ala Pro Ala Asp Ala Leu Ala Ala Gln Val
105 110 115gaa aac ctt att ccc ttg tgt gaa gag ggc ctg ctg tgc ggc att cac 499Glu Asn Leu Ile Pro Leu Cys Glu Glu Gly Leu Leu Cys Gly Ile His
120 125 130ctc gag ggt cct ttc atc aac gca tgc cgt tgt ggt gct caa aac ccg 547Leu Glu Gly Pro Phe Ile Asn Ala Cys Arg Cys Gly Ala Gln Asn Pro
135 140 145gat ttt att ttt ccc ggc aac cca aca gat ctt gcc cag gtg atc cat 595Asp Phe Ile Phe Pro Gly Asn Pro Thr Asp Leu Ala Gln Val Ile His150 155 160 165gcg gga aaa ggt tgg atc aaa tcg atc aca gta gcg ccg gaa act gac 643Ala Gly Lys Gly Trp Ile Lys Ser Ile Thr Val Ala Pro Glu Thr Asp
170 175 180aat ctt act gag ctt ctc gat ctc tgc gca gcg cac cac atc att gct 691Asn Leu Thr Glu Leu Leu Asp Leu Cys Ala Ala His His Ile Ile Ala
185 190 195tcc ttc ggg cac act gat gca gat ttt gat acc act acc agc gca att 739Ser Phe Gly His Thr Asp Ala Asp Phe Asp Thr Thr Thr Ser Ala Ile
200 205 210gcc ttg gct aaa gag aaa aat gtg acg gtc acg gct acg cat ttg ttc 787Ala Leu Ala Lys Glu Lys Asn Val Thr Val Thr Ala Thr His Leu Phe
215 220 225aat gcg atg cct ccg ctg cat cat agg gat ccc ggc agc gtg ggc gct 835Asn Ala Met Pro Pro Leu His His Arg Asp Pro Gly Ser Val Gly Ala230 235 240 245ttg ctt gct gcg gca cgt gcc ggg gac gca tat gtt gag ttg atc gcc 883Leu Leu Ala Ala Ala Arg Ala Gly Asp Ala Tyr Val Glu Leu Ile Ala
250 255 260gac ggc gtg cat ttg gcc gat gga acg gtc gat cta gct cgt tcc aac 931Asp Gly Val His Leu Ala Asp Gly Thr Val Asp Leu Ala Arg Ser Asn
265 270 275aac gcc ttt ttc atc acg gac gcc atg gaa gcc gcc gga atg cca gac 979Asn Ala Phe Phe Ile Thr Asp Ala Met Glu Ala Ala Gly Met Pro Asp
280 285 290ggt gag tac att ttg ggc gtt ttg aac gtc acc gtc acc gat ggc gtc 1027Gly Glu Tyr Ile Leu Gly Val Leu Asn Val Thr Val Thr Asp Gly Val
295 300 305gcc cgt ctg cgc gat ggc ggc gcc atc gcc ggg ggt acc agc aca cta 1075Ala Arg Leu Arg Asp Gly Gly Ala Ile Ala Gly Gly Thr Set Thr Leu310 315 320 325gcg agt cag ttc gtg cac cac gtg cgc agg ggt atg acg ctt atc gac 1123Ala Ser Gln Phe Val His His Val Arg Arg Gly Met Thr Leu Ile Asp
330 335 340gcg acc ctc cac acc tca acc gtc gcc gcc aaa att ctc gga ctt agc 1171Ala Thr Leu His Thr Ser Thr Val Ala Ala Lys Ile Leu Gly Leu Ser
345 350 355gat cac gaa atc gtt aaa tcc aac cct gta aat ttt gtg gtc ttt gac 1219Asp His Glu Ile Val Lys Ser Asn Pro Val Asn Phe Val Val Phe Asp
360 365 370tca aac ggc cag tta caa cag gtc cat tta gac cat caa gta att 1264Ser Asn Gly Gln Leu Gln Gln Val His Leu Asp His Gln Val Ile
375 380 385taaatacgag caaaactttc ctg 1287<210>416<211>388<212>PRT<213>谷氨酸棒杆菌<400>416Met Ala Glu Val Val His Tyr Gln Glu Asn Ala Gly Gln Ala Val Lys1 5 l0 15Lys Ile Glu Gly Arg Ile Val Thr Pro His Gly Val Ile Asp Gly Phe
20 25 30Leu Gln Leu Glu Asn Gly Ile Ile Thr Glu Leu Ser Gly Glu Pro Ala
35 40 45Pro Lys Asn Ala Gly Phe His Pro Glu Leu Pro Thr Ile Val Pro Ser
50 55 60Phe Ile Asp Leu His Asn His Gly Gly Asn Gly Gly Ala Phe Pro Thr65 70 75 80Gly Thr Gln Asp Gln Ala Arg Asn Ala Ala Gln Tyr His Arg Glu His
85 90 95Gly Thr Thr Val Met Leu Ala Ser Met Val Ser Ala Pro Ala Asp Ala
100 105 110Leu Ala Ala Gln Val Glu Asn Leu Ile Pro Leu Cys Glu Glu Gly Leu
115 120 125Leu Cys Gly Ile His Leu Glu Gly Pro Phe Ile Asn Ala Cys Arg Cys
130 135 140Gly Ala Gln Asn Pro Asp Phe Ile Phe Pro Gly Asn Pro Thr Asp Leu145 150 155 160Ala Gln Val Ile His Ala Gly Lys Gly Trp Ile Lys Ser Ile Thr Val
165 170 175Ala Pro Glu Thr Asp Asn Leu Thr Glu Leu Leu Asp Leu Cys Ala Ala
180 185 190His His Ile Ile Ala Ser Phe Gly His Thr Asp Ala Asp Phe Asp Thr
195 200 205Thr Thr Ser Ala Ile Ala Leu Ala Lys Glu Lys Asn Val Thr Val Thr
210 215 220Ala Thr His Leu Phe Asn Ala Met Pro Pro Leu His His Arg Asp Pro225 230 235 240Gly Ser Val Gly Ala Leu Leu Ala Ala Ala Arg Ala Gly Asp Ala Tyr
245 250 255Val Glu Leu Ile Ala Asp Gly Val His Leu Ala Asp Gly Thr Val Asp
260 265 270Leu Ala Arg Ser Asn Asn Ala Phe Phe Ile Thr Asp Ala Met Glu Ala
275 280 285Ala Gly Met Pro Asp Gly Glu Tyr Ile Leu Gly Val Leu Asn Val Thr
290 295 300Val Thr Asp Gly Val Ala Arg Leu Arg Asp Gly Gly Ala Ile Ala Gly305 310 315 320Gly Thr Ser Thr Leu Ala Ser Gln Phe Val His His Val Arg Arg Gly
325 330 335Met Thr Leu Ile Asp Ala Thr Leu His Thr Ser Thr Val Ala Ala Lys
340 345 350Ile Leu Gly Leu Ser Asp His Glu Ile Val Lys Ser Asn Pro Val Asn
355 360 365Phe Val Val Phe Asp Ser Asn Gly Gln Leu Gln Gln Val His Leu Asp
370 375 380His Gln Val Ile385<210>417<211>1584<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1561)<223>RXN01752<400>417gaaatgacgt gaccatcgat accaataccc aattgaaaga tcttgacctg gtcagccaag 60ttggccgtca gatcgtggca gaacaacagg tggggaggtc atg atg gaa caa gat 115
Met Met Glu Gln Asp
1 5ctc agc tac cgt gaa att ctt ccc ctc aac gcg agt gag gag aag aaa 163Leu Ser Tyr Arg Glu Ile Leu Pro Leu Asn Ala Ser Glu Glu Lys Lys
10 15 20aag gct gca ctg att gat gcc att gaa ggg tta agg gtg cgc gat ccg 211Lys Ala Ala Leu Ile Asp Ala Ile Glu Gly Leu Arg Val Arg Asp Pro
25 30 35cta ctc tct gcc tcg att gca ttt act aga ggg cag aaa gtc gcc ttc 259Leu Leu Ser Ala Ser Ile Ala Phe Thr Arg Gly Gln Lys Val Ala Phe
40 45 50att gct gtg gtg gtg ggc ttt atc ttg atg ctc att ttt gct cgg caa 307Ile Ala Val Val Val Gly Phe Ile Leu Met Leu Ile Phe Ala Arg Gln
55 60 65gca gca ctt att gga ctg tca gca acg tgt acg ttc atg tac ctc att 355Ala Ala Leu Ile Gly Leu Ser Ala Thr Cys Thr Phe Met Tyr Leu Ile70 75 80 85aca ttg ttg gac aga ttt atc atg ttt tcc aga ggt atc cgc gcg gaa 403Thr Leu Leu Asp Arg Phe Ile Met Phe Ser Arg Gly Ile Arg Ala Glu
90 95 100tcc atc atc cag gta tcg gat gaa gat gcg ctg gct ttc cct gag gac 451Ser Ile Ile Gln Val Ser Asp Glu Asp Ala Leu Ala Phe Pro Glu Asp
105 110 115aag ctg aaa acc tac acg gtg ttg gtg ccc gcc tat ggc gaa cct gag 499Lys Leu Lys Thr Tyr Thr Val Leu Val Pro Ala Tyr Gly Glu Pro Glu
120 125 130gtg att gcg cag ctg ctg gca tcc atg cac gct ttt gat tac ccc aag 547Val Ile Ala Gln Leu Leu Ala Ser Met His Ala Phe Asp Tyr Pro Lys
135 140 145cat ctt ctg cag gta ttg ctc atg ttg gag gaa gat gat ctg ccc acg 595His Leu Leu Gln Val Leu Leu Met Leu Glu Glu Asp Asp Leu Pro Thr150 155 160 165atc gcc gcg gca gag gca gcg gga gtg gat cag gtg gca acg atc att 643Ile Ala Ala Ala Glu Ala Ala Gly Val Asp Gln Val Ala Thr Ile Ile
170 175 180aag gtg ccg cca gcg cag ccc cgc acc aag ccg aag gcc tgt aac tat 691Lys Val Pro Pro Ala Gln Pro Arg Thr Lys Pro Lys Ala Cys Asn Tyr
185 190 195gga ttg cac ttt gcc acg ggg gaa att gtc acg atc ttt gac gcg gaa 739Gly Leu His Phe Ala Thr Gly Glu Ile Val Thr Ile Phe Asp Ala Glu
200 205 210gac atg cca gat ccc ctc caa ctg cgt cgc gtg gtg gtg gca ttt gaa 787Asp Met Pro Asp Pro Leu Gln Leu Arg Arg Val Val Val Ala Phe Glu
215 220 225cgc tcg gct tcc aat acg gtg tgc gtc cag tca agg ttg tcg tat cga 835Arg Ser Ala Ser Asn Thr Val Cys Val Gln Ser Arg Leu Ser Tyr Arg230 235 240 245aac gcc agg cag aat ctg cta act gcg tgg ttc acc att gaa tat gac 883Asn Ala Arg Gln Asn Leu Leu Thr Ala Trp Phe Thr Ile Glu Tyr Asp
250 255 260gtg tgg ttt aac ttc ctg ctg cca ggc gtc atg cgc atg aac gca cct 931Val Trp Phe Asn Phe Leu Leu Pro Gly Val Met Arg Met Asn Ala Pro
265 270 275gtc cca ttg ggc ggt acc tcc aac cat ctg ctc acg ggt gtc ctg aaa 979Val Pro Leu Gly Gly Thr Ser Asn His Leu Leu Thr Gly Val Leu Lys
280 285 290gat ctc ggc gcg tgg gat cct ttc aat gtc aca gaa aat gcc gac ctc 1027Asp Leu Gly Ala Trp Asp Pro Phe Asn Val Thr Glu Asn Ala Asp Leu
295 300 305ggc gta ccc atc gcg gca aaa gga tat tcc acc gcg gtg ttg gat tcg 1075Gly Val Pro Ile Ala Ala Lys Gly Tyr Ser Thr Ala Val Leu Asp Ser310 315 320 325gtg acg tgg gag gaa gca aac tcc gac acc atc aac tgg ttg cgc cag 1123Val Thr Trp Glu Glu Ala Asn Ser Asp Thr Ile Asn Trp Leu Arg Gln
330 335 340cgt tct cgc tgg tac aag ggc tat ctg caa aca tgg ctt gtg tat atg 1171Arg Ser Arg Trp Tyr Lys Gly Tyr Leu Gln Thr Trp Leu Val Tyr Met
345 350 355cgc agg cca aag tgg tta gtc caa gag ctt ggc atc att cct gct gtg 1219Arg Arg Pro Lys Trp Leu Val Gln Glu Leu Gly Ile Ile Pro Ala Val
360 365 370cgt ttt acc ttc ctc atg gca ggc acc ccg atc att gcg gtg ctc aat 1267Arg Phe Thr Phe Leu Met Ala Gly Thr Pro Ile Ile Ala Val Leu Asn
375 380 385ctg ctc ttt tgg tac ttg tcg ctc acg tgg att ctg ggc cag ccc ggc 1315Leu Leu Phe Trp Tyr Leu Ser Leu Thr Trp Ile Leu Gly Gln Pro Gly390 395 400 405acc att gag cag atg ttc cca cct gcg gtg tac tac cca gcg ttg gtg 1363Thr Ile Glu Gln Met Phe Pro Pro Ala Val Tyr Tyr Pro Ala Leu Val
410 415 420tgt ttg gtg gtg gcc aat gct gcg acc atc ttt atg aat ctc att ggc 1411Cys Leu Val Val Ala Asn Ala Ala Thr Ile Phe Met Asn Leu Ile Gly
425 430 435tgc cgg gaa ggc cgc gac ccc ttg ctg ctc atc gcg gtt ctc acg ttc 1459Cys Arg Glu Gly Arg Asp Pro Leu Leu Leu Ile Ala Val Leu Thr Phe
440 445 450ccg ctg tat tgg ctg ctc atg agc att gca gcg ttg aaa ggc acg tgg 1507Pro Leu Tyr Trp Leu Leu Met Ser Ile Ala Ala Leu Lys Gly Thr Trp
455 460 465caa ttg atc acg cga cca tcc tat tgg gag aaa act gcc cac gga ttg 1555Gln Leu Ile Thr Arg Pro Ser Tyr Trp Glu Lys Thr Ala His Gly Leu470 475 480 485gag gcg taagcggtgc ccatcgtcaa acc 1584Glu Ala<210>418<211>487<212>PRT<213>谷氨酸棒杆菌<400>418Met Met Glu Gln Asp Leu Ser Tyr Arg Glu Ile Leu Pro Leu Asn Ala1 5 10 15Ser Glu Glu Lys Lys Lys Ala Ala Leu Ile Asp Ala Ile Glu Gly Leu
20 25 30Arg Val Arg Asp Pro Leu Leu Ser Ala Ser Ile Ala Phe Thr Arg Gly
35 40 45Gln Lys Val Ala Phe Ile Ala Val Val Val Gly Phe Ile Leu Met Leu
50 55 60Ile Phe Ala Arg Gln Ala Ala Leu Ile Gly Leu Ser Ala Thr Cys Thr65 70 75 80Phe Met Tyr Leu Ile Thr Leu Leu Asp Arg Phe Ile Met Phe Ser Arg
85 90 95Gly Ile Arg Ala Glu Ser Ile Ile Gln Val Ser Asp Glu Asp Ala Leu
100 105 110Ala Phe Pro Glu Asp Lys Leu Lys Thr Tyr Thr Val Leu Val Pro Ala
115 120 125Tyr Gly Glu Pro Glu Val Ile Ala Gln Leu Leu Ala Ser Met His Ala
130 135 140Phe Asp Tyr Pro Lys His Leu Leu Gln Val Leu Leu Met Leu Glu Glu145 150 155 160Asp Asp Leu Pro Thr Ile Ala Ala Ala Glu Ala Ala Gly Val Asp Gln
165 170 175Val Ala Thr Ile Ile Lys Val Pro Pro Ala Gln Pro Arg Thr Lys Pro
180 185 190Lys Ala Cys Asn Tyr Gly Leu His Phe Ala Thr Gly Glu Ile Val Thr
195 200 205Ile Phe Asp Ala Glu Asp Met Pro Asp Pro Leu Gln Leu Arg Arg Val
210 215 220Val Val Ala Phe Glu Arg Ser Ala Ser Asn Thr Val Cys Val Gln Ser225 230 235 240Arg Leu Ser Tyr Arg Asn Ala Arg Gln Asn Leu Leu Thr Ala Trp Phe
245 250 255Thr Ile Glu Tyr Asp Val Trp Phe Asn Phe Leu Leu Pro Gly Val Met
260 265 270Arg Met Asn Ala Pro Val Pro Leu Gly Gly Thr Ser Asn His Leu Leu
275 280 285Thr Gly Val Leu Lys Asp Leu Gly Ala Trp Asp Pro Phe Asn Val Thr
290 295 300Glu Asn Ala Asp Leu Gly Val Pro Ile Ala Ala Lys Gly Tyr Ser Thr305 310 315 320Ala Val Leu Asp Ser Val Thr Trp Glu Glu Ala Asn Ser Asp Thr Ile
325 330 335Asn Trp Leu Arg Gln Arg Ser Arg Trp Tyr Lys Gly Tyr Leu Gln Thr
340 345 350Trp Leu Val Tyr Met Arg Arg Pro Lys Trp Leu Val Gln Glu Leu Gly
355 360 365Ile Ile Pro Ala Val Arg Phe Thr Phe Leu Met Ala Gly Thr Pro Ile
370 375 380Ile Ala Val Leu Asn Leu Leu Phe Trp Tyr Leu Ser Leu Thr Trp Ile385 390 395 400Leu Gly Gln Pro Gly Thr Ile Glu Gln Met Phe Pro Pro Ala Val Tyr
405 410 415Tyr Pro Ala Leu Val Cys Leu Val Val Ala Asn Ala Ala Thr Ile Phe
420 425 430Met Asn Leu Ile Gly Cys Arg Glu Gly Arg Asp Pro Leu Leu Leu Ile
435 440 445Ala Val Leu Thr Phe Pro Leu Tyr Trp Leu Leu Met Ser Ile Ala Ala
450 455 460Leu Lys Gly Thr Trp Gln Leu Ile Thr Arg Pro Ser Tyr Trp Glu Lys465 470 475 480Thr Ala His Gly Leu Glu Ala
485<210>419<21l>689<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(19)..(666)<223>FRXA01839<400>419ttcctgctgc caggtgtc atg cgc atg aac gca cct gtc cca ttg ggc ggt 51
Met Arg Met Asn Ala Pro Val Pro Leu Gly Gly
1 5 10acc tcc aac cat ctg ctc acg ggt gtc ctg aaa gat ctc ggc gcg tgg 99Thr Ser Asn His Leu Leu Thr Gly Val Leu Lys Asp Leu Gly Ala Trp
15 20 25gat cct ttc aat gtc aca gaa gat gcg gac ctc ggc gta cgc atc gcg 147Asp Pro Phe Asn Val Thr Glu Asp Ala Asp Leu Gly Val Arg Ile Ala
30 35 40gca aag gga tat tcc acc gcg gtg ttg gat tcg gtg acg tgg gag gaa 195Ala Lys Gly Tyr Set Thr Ala Val Leu Asp Ser Val Thr Trp Glu Glu
45 50 55gca aac tcc gac acc atc aac tgg ttg cgc cag cgt tct cgc tgg tac 243Ala Asn Ser Asp Thr Ile Asn Trp Leu Arg Gln Arg Ser Arg Trp Tyr60 65 70 75aag ggc tat ctg caa aca tgg ctt gtg tat atg cgc agg cca aag tgg 291Lys Gly Tyr Leu Gln Thr Trp Leu Val Tyr Met Arg Arg Pro Lys Trp
80 85 90tta gtc caa gag ctt ggc atc att cct gct gtg cgt ttt acc ttc ctc 339Leu Val Gln Glu Leu Gly Ile Ile Pro Ala Val Arg Phe Thr Phe Leu
95 100 105atg gca ggc acc ccg atc att gcg gtg ctc aat ctg ctc ttt tgg tac 387Met Ala Gly Thr Pro Ile Ile Ala Val Leu Asn Leu Leu Phe Trp Tyr
110 115 120ttg tcg ctc acg tgg att ctg ggc cag ccc ggc acc att gag cag atg 435Leu Ser Leu Thr Trp Ile Leu Gly Gln Pro Gly Thr Ile Glu Gln Met
125 130 135ttc cca cct gcg gtg tac tac cca gcg ttg gtg tgt ttg gtg gtg gcc 483Phe Pro Pro Ala Val Tyr Tyr Pro Ala Leu Val Cys Leu Val Val Ala140 145 150 155aat gct gcg acc atc ttt atg aat ctc att ggc tgc cgg gaa ggc cgc 531Asn Ala Ala Thr Ile Phe Met Asn Leu Ile Gly Cys Arg Glu Gly Arg
160 165 170gac ccc ttg ctg ctc atc gcg gtt ctc acg ttc ccg ctg tat tgg ctg 579Asp Pro Leu Leu Leu Ile Ala Val Leu Thr Phe Pro Leu Tyr Trp Leu
175 180 185ctc atg agc att gca gcg ttg aaa ggc acg tgg caa ttg atc acg cga 627Leu Met Ser Ile Ala Ala Leu Lys Gly Thr Trp Gln Leu Ile Thr Arg
190 195 200cca tcc tat tgg gag aaa act gcc cac gga ttg gag gcg taagcggtgc 676Pro Ser Tyr Trp Glu Lys Thr Ala His Gly Leu Glu Ala
205 210 215ccatcgtcaa acc 689<210>420<211>216<212>PRT<213>谷氨酸棒杆菌<400>420Met Arg Met Asn Ala Pro Val Pro Leu Gly Gly Thr Ser Asn His Leu1 5 10 15Leu Thr Gly Val Leu Lys Asp Leu Gly Ala Trp Asp Pro Phe Asn Val
20 25 30Thr Glu Asp Ala Asp Leu Gly Val Arg Ile Ala Ala Lys Gly Tyr Ser
35 40 45Thr Ala Val Leu Asp Ser Val Thr Trp Glu Glu Ala Asn Ser Asp Thr
50 55 60Ile Asn Trp Leu Arg Gln Arg Ser Arg Trp Tyr Lys Gly Tyr Leu Gln65 70 75 80Thr Trp Leu Val Tyr Met Arg Arg Pro Lys Trp Leu Val Gln Glu Leu
85 90 95Gly Ile Ile Pro Ala Val Arg Phe Thr Phe Leu Met Ala Gly Thr Pro
100 105 110Ile Ile Ala Val Leu Asn Leu Leu Phe Trp Tyr Leu Ser Leu Thr Trp
115 120 125Ile Leu Gly Gln Pro Gly Thr Ile Glu Gln Met Phe Pro Pro Ala Val
130 135 140Tyr Tyr Pro Ala Leu Val Cys Leu Val Val Ala Asn Ala Ala Thr Ile145 150 155 160Phe Met Asn Leu Ile Gly Cys Arg Glu Gly Arg Asp Pro Leu Leu Leu
165 170 175Ile Ala Val Leu Thr Phe Pro Leu Tyr Trp Leu Leu Met Ser Ile Ala
180 185 190Ala Leu Lys Gly Thr Trp Gln Leu Ile Thr Arg Pro Ser Tyr Trp Glu
195 200 205Lys Thr Ala His Gly Leu Glu Ala
210 215<210>421<211>1050<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1027)<223>RXA01859<400>421tacgcccagg gtttccatat tggtaaatct aagccgattg atgaatttat agctacttat 60ctcgagacga accaaaccgc tacctggggg taggaagaat atg aaa aag aag agc 115
Met Lys Lys Lys Ser
1 5ttt cca atc gca aga gtc atc ggt atc ggc gtc ctt ggc atc gcc ggg 163Phe Pro Ile Ala Arg Val Ile Gly Ile Gly Val Leu Gly Ile Ala Gly
10 15 20atg gga ata ttg ttg cta tgg ctt gca gtt acc ctg tct gat cca gca 211Met Gly Ile Leu Leu Leu Trp Leu Ala Val Thr Leu Ser Asp Pro Ala
25 30 35tca ccg ggt gcc aaa gaa acc gaa gtc ttt gat agg tgg aaa gtg ctc 259Ser Pro Gly Ala Lys Glu Thr Glu Val Phe Asp Arg Trp Lys Val Leu
40 45 50ttt gat gac tat att cca cca gtc agg gta ttg gtt gct gcg att atc 307Phe Asp Asp Tyr Ile Pro Pro Val Arg Val Leu Val Ala Ala Ile Ile
55 60 65gtt gca tta att ttc gtc ttt gtc gct gcc aca gtg gaa cga acc gta 355Val Ala Leu Ile Phe Val Phe Ile Ala Ala Thr Val Glu Arg Thr Val70 75 80 85acc aac cgc tac cga agc tcc gta gac ggc gaa aga gtg cca tta gcg 403Thr Asn Arg Tyr Arg Ser Ser Val Asp Gly Glu Arg Val Pro Leu Ala
90 95 100ccg aag att gtg atg gca gaa acc cga ggg gta ttt cat gga ccg att 451Pro Lys Ile Val Met Ala Glu Thr Arg Gly Val Phe His Gly Pro Ile
105 110 115acc att aac gtg ctc gtg cca gca cac aat gag gcg gaa aga att act 499Thr Ile Asn Val Leu Val Pro Ala His Asn Glu Ala Glu Arg Ile Thr
120 125 130gga aca att cag gca ttg aaa tca caa cat gag cct cca gaa cgc atc 547Gly Thr Ile Gln Ala Leu Lys Ser Gln His Glu Pro Pro Glu Arg Ile
135 140 145gtt gta gtt gcc gat aat tgc act gat gaa act acg gaa tta gcc cgt 595Val Val Val Ala Asp Asn Cys Thr Asp Glu Thr Thr Glu Leu Ala Arg150 155 160 165gct gag gga gtg gag gtc ttg gaa aca gtc aat aat aag ttt aag aag 643Ala Glu Gly Val Glu Val Leu Glu Thr Val Asn Asn Lys Phe Lys Lys
170 175 180gcc gga gga ctc aat cag gct ttg agc cgg atg ctt ccc aca ttg ggg 691Ala Gly Gly Leu Asn Gln Ala Leu Ser Arg Met Leu Pro Thr Leu Gly
185 190 195gag aat gac att gtg atg atc gtt gac gct gat aca gca ctt gat caa 739Glu Asn Asp Ile Val Met Ile Val Asp Ala Asp Thr Ala Leu Asp Gln
200 205 210ggt ttc ctc aag gaa gca cgg cgc cgc ttt gag tct gat cgc gct cta 787Gly Phe Leu Lys Glu Ala Arg Arg Arg Phe Glu Ser Asp Arg Ala Leu
215 220 225atg gcc gtg ggc gga ttg ttc tac ggt gag tca ggc tcc gga tgg ctt 835Met Ala Val Gly Gly Leu Phe Tyr Gly Glu Ser Gly Ser Gly Trp Leu230 235 240 245ggc caa tat cag cgc aac gaa tac acc cgt tat agc cgt gac atc tat 883Gly Gln Tyr Gln Arg Asn Glu Tyr Thr Arg Tyr Ser Arg Asp Ile Tyr
250 255 260cga cgc cgc gga cgt gtg ttt gtt ttg act gga aca gcg tcg gct ttt 931Arg Arg Arg Gly Arg Val Phe Val Leu Thr Gly Thr Ala Ser Ala Phe
265 270 275cgg cca cgc ggc ctg cgg aca gta gcg gaa tca cgc ggg aca ttg atc 979Arg Pro Arg Gly Leu Arg Thr Val Ala Glu Ser Arg Gly Thr Leu Ile
280 285 290ccc gga cgt aaa gcc gat gtt tat gac acc gcg ggc gtt gac cga aga 1027Pro Gly Arg Lys Ala Asp Val Tyr Asp Thr Ala Gly Val Asp Arg Arg
295 300 305taatgagttg accctggctt tga 1050<210>422<211>309<212>PRT<213>谷氨酸棒杆菌<400>422Met Lys Lys Lys Ser Phe Pro Ile Ala Arg Val Ile Gly Ile Gly Val1 5 10 15Leu Gly Ile Ala Gly Met Gly Ile Leu Leu Leu Trp Leu Ala Val Thr
20 25 30Leu Ser Asp Pro Ala Ser Pro Gly Ala Lys Glu Thr Glu Val Phe Asp
35 40 45Arg Trp Lys Val Leu Phe Asp Asp Tyr Ile Pro Pro Val Arg Val Leu
50 55 60Val Ala Ala Ile Ile Val Ala Leu Ile Phe Val Phe Ile Ala Ala Thr65 70 75 80Val Glu Arg Thr Val Thr Asn Arg Tyr Arg Ser Ser Val Asp Gly Glu
85 90 95Arg Val Pro Leu Ala Pro Lys Ile Val Met Ala Glu Thr Arg Gly Val
100 105 110Phe His Gly Pro Ile Thr Ile Asn Val Leu Val Pro Ala His Asn Glu
115 120 125Ala Glu Arg Ile Thr Gly Thr Ile Gln Ala Leu Lys Ser Gln His Glu
130 135 140Pro Pro Glu Arg Ile Val Val Val Ala Asp Asn Cys Thr Asp Glu Thr145 150 155 160Thr Glu Leu Ala Arg Ala Glu Gly Val Glu Val Leu Glu Thr Val Asn
165 170 175Asn Lys Phe Lys Lys Ala Gly Gly Leu Asn Gln Ala Leu Ser Arg Met
180 185 190Leu Pro Thr Leu Gly Glu Asn Asp Ile Val Met Ile Val Asp Ala Asp
195 200 205Thr Ala Leu Asp Gln Gly Phe Leu Lys Glu Ala Arg Arg Arg Phe Glu
210 215 220Ser Asp Arg Ala Leu Met Ala Val Gly Gly Leu Phe Tyr Gly Glu Ser225 230 235 240Gly Ser Gly Trp Leu Gly Gln Tyr Gln Arg Asn Glu Tyr Thr Arg Tyr
245 250 255Ser Arg Asp Ile Tyr Arg Arg Arg Gly Arg Val Phe Val Leu Thr Gly
260 265 270Thr Ala Ser Ala Phe Arg Pro Arg Gly Leu Arg Thr Val Ala Glu Ser
275 280 285Arg Gly Thr Leu Ile Pro Gly Arg Lys Ala Asp Val Tyr Asp Thr Ala
290 295 300Gly Val Asp Arg Arg305<210>423<211>882<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(859)<223>RXA00042<400>423gtggtctttg actcaaacgg ccagttacaa caggtccatt tagaccatca agtaatttaa 60atacgagcaa aactttcctg ataataaaag gagtccgacc atg gac atc atc atc 115
Met Asp Ile Ile Ile
1 5tgc aaa gac gag caa gaa gtc ggc aaa gca gcg gca gcc ctg atc gca 163Cys Lys Asp Glu Gln Glu Val Gly Lys Ala Ala Ala Ala Leu Ile Ala
10 15 20ccc ttc gca act aag ggc gga acc ttg ggg ctt gca act gga tcg tca 211Pro Phe Ala Thr Lys Gly Gly Thr Leu Gly Leu Ala Thr Gly Ser Ser
25 30 35cct ttg agc acc tac caa gag ctc att cgc atg tat gaa gct ggg gaa 259Pro Leu Ser Thr Tyr Gln Glu Leu Ile Arg Met Tyr Glu Ala Gly Glu
40 45 50gtg tca ttc aag aac tgc aag gca ttc ttg ttg gat gaa tac gtg gga 307Val Ser Phe Lys Asn Cys Lys Ala Phe Leu Leu Asp Glu Tyr Val Gly
55 60 65tta acg cgc gac gat gaa aac agc tac ttc aaa acc att cgt aaa gag 355Leu Thr Arg Asp Asp Glu Asn Ser Tyr Phe Lys Thr Ile Arg Lys Glu70 75 80 85ttc act gac cac atc gac atc gtt gat gaa gag gtc tac agc cca gat 403Phe Thr Asp His Ile Asp Ile Val Asp Glu Glu Val Tyr Ser Pro Asp
90 95 100ggt gca aac cct gat cca tac gaa gca gct gca gag tat gag gca aag 451Gly Ala Asn Pro Asp Pro Tyr Glu Ala Ala Ala Glu Tyr Glu Ala Lys
105 110 115atc gct gca gaa tcc gtt gat gtt caa atc ctt ggc atc ggc gga aac 499Ile Ala Ala Glu Ser Val Asp Val Gln Ile Leu Gly Ile Gly Gly Asn
120 125 130ggc cac atc gct ttc aat gag cca tca tct tct ctg tca gga ctg aca 547Gly His Ile Ala Phe Asn Glu Pro Ser Ser Ser Leu Ser Gly Leu Thr
135 140 145aag gtc cag gcg ctg cac cct aaa act gtg gag gac aac gct cga ttc 595Lys Val Gln Ala Leu His Pro Lys Thr Val Glu Asp Asn Ala Arg Phe150 155 160 165ttc aac acc atc gaa gag gtc cca acc cac gcc ctc acc cag ggt ttg 643Phe Asn Thr Ile Glu Glu Val Pro Thr His Ala Leu Thr Gln Gly Leu
170 175 180ggc act ttg tcc cgc gcg caa aac atc gtg ttg gtg gca act ggt gaa 691Gly Thr Leu Ser Arg Ala Gln Asn Ile Val Leu Val Ala Thr Gly Glu
185 190 195gga aaa gcc gac gcc atc cgc gga act gtg gaa ggc cca ctg acc gcc 739Gly Lys Ala Asp Ala Ile Arg Gly Thr Val Glu Gly Pro Leu Thr Ala
200 205 210atg tgc cca ggt tcc atc ctg cag atg cac aac aat gcc acc atc atc 787Met Cys Pro Gly Ser Ile Leu Gln Met His Asn Asn Ala Thr Ile Ile
215 220 225gtt gat gaa gca gca gca tcc aag ctg gaa aac gct gat cac tac cgt 835Val Asp Glu Ala Ala Ala Ser Lys Leu Glu Asn Ala Asp His Tyr Arg230 235 240 245ctc atg gag caa tta aag ctg cgc tagaaacaaa aaggaaagta gtg 882Leu Met Glu Gln Leu Lys Leu Arg
250<210>424<211>253<212>PRT<213>谷氨酸棒杆菌<400>424Met Asp Ile Ile Ile Cys Lys Asp Glu Gln Glu Val Gly Lys Ala Ala1 5 10 15Ala Ala Leu Ile Ala Pro Phe Ala Thr Lys Gly Gly Thr Leu Gly Leu
20 25 30Ala Thr Gly Ser Ser Pro Leu Ser Thr Tyr Gln Glu Leu Ile Arg Met
35 40 45Tyr Glu Ala Gly Glu Val Ser Phe Lys Asn Cys Lys Ala Phe Leu Leu
50 55 60Asp Glu Tyr Val Gly Leu Thr Arg Asp Asp Glu Asn Ser Tyr Phe Lys65 70 75 80Thr Ile Arg Lys Glu Phe Thr Asp His Ile Asp Ile Val Asp Glu Glu
85 90 95Val Tyr Ser Pro Asp Gly Ala Asn Pro Asp Pro Tyr Glu Ala Ala Ala
100 105 110Glu Tyr Glu Ala Lys Ile Ala Ala Glu Ser Val Asp Val Gln Ile Leu
115 120 125Gly Ile Gly Gly Asn Gly His Ile Ala Phe Asn Glu Pro Ser Ser Ser
130 135 140Leu Ser Gly Leu Thr Lys Val Gln Ala Leu His Pro Lys Thr Val Glu145 150 155 160Asp Asn Ala Arg Phe Phe Asn Thr Ile Glu Glu Val Pro Thr His Ala
165 170 175Leu Thr Gln Gly Leu Gly Thr Leu Ser Arg Ala Gln Asn Ile Val Leu
180 185 190Val Ala Thr Gly Glu Gly Lys Ala Asp Ala Ile Arg Gly Thr Val Glu
195 200 205Gly Pro Leu Thr Ala Met Cys Pro Gly Ser Ile Leu Gln Met His Asn
210 215 220Asn Ala Thr Ile Ile Val Asp Glu Ala Ala Ala Ser Lys Leu Glu Asn225 230 235 240Ala Asp His Tyr Arg Leu Met Glu Gln Leu Lys Leu Arg
245 250<210>425<211>1998<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1975)<223>RXA01482<400>425ttgcgtgctg caacttaatt atggtcctcc cagctcagtg tgctgtgtgg attgtttatt 60ctcgtccatt aagtgatcga gaaaaagttg ttgtaaagtc atg cgc atg tgt gga 115
Met Arg Met Cys Gly
1 5att gtt gga tat att ggc caa gcg ggc gac tcc cgt gat tac ttt gct 163Ile Val Gly Tyr Ile Gly Gln Ala Gly Asp Ser Arg Asp Tyr Phe Ala
10 15 20cta gat gta gtt gtt gaa gga cta cgt cgc ctg gaa tac cgc gga tat 211Leu Asp Val Val Val Glu Gly Leu Arg Arg Leu Glu Tyr Arg Gly Tyr
25 30 35gac tcc gca ggt att gct att cac gcc aat ggt gag att agc tac cga 259Asp Ser Ala Gly Ile Ala Ile His Ala Asn Gly Glu Ile Ser Tyr Arg
40 45 50aag aag gcc gga aag gtt gct gca cta gat gca gaa atc gct aaa gca 307Lys Lys Ala Gly Lys Val Ala Ala Leu Asp Ala Glu Ile Ala Lys Ala
55 60 65cct ctt cca gat tct att ttg gga att gga cac acc cgt tgg gca act 355Pro Leu Pro Asp Ser Ile Leu Gly Ile Gly His Thr Arg Trp Ala Thr70 75 80 85cat ggt ggc cca acc gat gtc aac gct cac ccc cac gtt gtt tcc aat 403His Gly Gly Pro Thr Asp Val Asn Ala His Pro His Val Val Ser Asn
90 95 100ggc aag ctt gcc gta gta cac aac ggc atc atc gaa aac ttt gcg gaa 451Gly Lys Leu Ala Val Val His Asn Gly Ile Ile Glu Asn Phe Ala Glu
105 110 115ctg cgc tct gag ctt tcc gct aag ggc tac aac ttt gta tcc gat acc 499Leu Arg Ser Glu Leu Ser Ala Lys Gly Tyr Asn Phe Val Ser Asp Thr
120 125 130gat acc gaa gtt gct gct tct ttg ctt gct gaa att tac aat act cag 547Asp Thr Glu Val Ala Ala Ser Leu Leu Ala Glu Ile Tyr Asn Thr Gln
135 140 145gca aac ggt gac ctc acc ctt gct atg cag ctg acc ggt cag cgc ctt 595Ala Asn Gly Asp Leu Thr Leu Ala Met Gln Leu Thr Gly Gln Arg Leu150 155 160 165gag ggt gct ttc acc ctg cta gct att cat gct gat cac gat gac cgc 643Glu Gly Ala Phe Thr Leu Leu Ala Ile His Ala Asp His Asp Asp Arg
170 175 180atc gtt gca gct cgt cgt aac tct cct ttg gtt atc ggc gtc ggc gag 691Ile Val Ala Ala Arg Arg Asn Ser Pro Leu Val Ile Gly Val Gly Glu
185 190 195ggc gag aac ttc ctc gga tct gac gtt tct ggc ttt att gat tac acc 739Gly Glu Asn Phe Leu Gly Ser Asp Val Ser Gly Phe Ile Asp Tyr Thr
200 205 210cgc aag gct gta gag ctg gct aat gac cag gtt gtt acc atc acc gct 787Arg Lys Ala Val Glu Leu Ala Asn Asp Gln Val Val Thr Ile Thr Ala
215 220 225gat gat tac gcc atc acc aac ttt gat gga tca gaa gca gtt ggc aag 835Asp Asp Tyr Ala Ile Thr Asn Phe Asp Gly Ser Glu Ala Val Gly Lys230 235 240 245cct ttc gac gtg gag tgg gac gct gca gct gct gaa aag ggt ggc ttc 883Pro Phe Asp Val Glu Trp Asp Ala Ala Ala Ala Glu Lys Gly Gly Phe
250 255 260ggt tcc ttc atg gag aag gaa atc cac gat cag cca gca gct gtt cgc 931Gly Ser Phe Met Glu Lys Glu Ile His Asp Gln Pro Ala Ala Val Arg
265 270 275gat acc ctg atg ggc cgt ctt gat gaa gat ggc aag ctc gtt ctt gat 979Asp Thr Leu Met Gly Arg Leu Asp Glu Asp Gly Lys Leu Val Leu Asp
280 285 290gag ctg cgc atc gat gaa gct att ctg cgt agt gtc gac aag atc gtc 1027Glu Leu Arg Ile Asp Glu Ala Ile Leu Arg Ser Val Asp Lys Ile Val
295 300 305att gtt gct tgt ggt act gca gct tat gca ggc cag gtt gct cgt tac 1075Ile Val Ala Cys Gly Thr Ala Ala Tyr Ala Gly Gln Val Ala Arg Tyr310 315 320 325gcc att gag cac tgg tgc cgc atc cca acc gag gtg gag ctg gct cac 1123Ala Ile Glu His Trp Cys Arg Ile Pro Thr Glu Val Glu Leu Ala His
330 335 340gag ttc cgt tac cgc gac cca atc ctc aac gag aag acc ctt gtt gtg 1171Glu Phe Arg Tyr Arg Asp Pro Ile Leu Asn Glu Lys Thr Leu Val Val
345 350 355gca ttg tcc cag tcc ggc gag acc atg gat acc ctc atg gct gtt cgc 1219Ala Leu Ser Gln Ser Gly Glu Thr Met Asp Thr Leu Met Ala Val Arg
360 365 370cac gca cgt gag cag ggt gcc aag gtt gtt gct att tgt aac act gtt 1267His Ala Arg Glu Gln Gly Ala Lys Val Val Ala Ile Cys Asn Thr Val
375 380 385gga tcc act ctt cca cgt gaa gca gat gcg tcc ctg tac acc tac gct 1315Gly Ser Thr Leu Pro Arg Glu Ala Asp Ala Ser Leu Tyr Thr Tyr Ala390 395 400 405ggc cct gag atc gct gtg gcg tcc acc aag gcg ttc ttg gct cag atc 1363Gly Pro Glu Ile Ala Val Ala Ser Thr Lys Ala Phe Leu Ala Gln Ile
410 415 420act gct tct tac ttg ctt ggc ctg tac ttg gct cag ctg cgc ggc aac 1411Thr Ala Ser Tyr Leu Leu Gly Leu Tyr Leu Ala Gln Leu Arg Gly Asn
425 430 435aag ttc gct gat gag gtt tct tcc att ctg gac agc ctg cgt gag atg 1459Lys Phe Ala Asp Glu Val Ser Ser Ile Leu Asp Ser Leu Arg Glu Met
440 445 450cct gag aag att cag cag gtc atc gat gca gaa gag cag atc aag aag 1507Pro Glu Lys Ile Gln Gln Val Ile Asp Ala Glu Glu Gln Ile Lys Lys
455 460 465ctt ggc caa gat atg gca gat gct aag tct gtg ctg ttc ctg ggc cgc 1555Leu Gly Gln Asp Met Ala Asp Ala Lys Ser Val Leu Phe Leu Gly Arg470 475 480 485cac gtt ggt ttc cca gtt gcg ctt gag ggt gcg ttg aag ctc aag gag 1603His Val Gly Phe Pro Val Ala Leu Glu Gly Ala Leu Lys Leu Lys Glu
490 495 500atc gca tac ctg cac gct gaa ggt ttc gct gca ggc gag ctc aag cac 1651Ile Ala Tyr Leu His Ala Glu Gly Phe Ala Ala Gly Glu Leu Lys His
505 510 515ggc cca att gct ttg gtt gag gaa ggc cag ccg atc ttc gtt atc gtg 1699Gly Pro Ile Ala Leu Val Glu Glu Gly Gln Pro Ile Phe Val Ile Val
520 525 530cct tca cct cgt ggt cgc gat tcc ctg cac tcc aag gtt gtc tcc aac 1747Pro Ser Pro Arg Gly Arg Asp Ser Leu His Ser Lys Val Val Ser Asn
535 540 545att cag gag atc cgt gca cgt ggc gct gtc acc atc gtg att gca gag 1795Ile Gln Glu Ile Arg Ala Arg Gly Ala Val Thr Ile Val Ile Ala Glu550 555 560 565gaa ggc gat gag gct gtc aac gat tac gcc aac ttc atc atc cgc att 1843Glu Gly Asp Glu Ala Val Asn Asp Tyr Ala Asn Phe Ile Ile Arg Ile
570 575 580cct cag gcc cca acc ctg atg cag cct ctg ctg tcc acc gtg cct ctg 1891Pro Gln Ala Pro Thr Leu Met Gln Pro Leu Leu Ser Thr Val Pro Leu
585 590 595cag atc ttt gcg tgc gct gtg gca acc gca aag ggc tac aac gtg gat 1939Gln Ile Phe Ala Cys Ala Val Ala Thr Ala Lys Gly Tyr Asn Val Asp
600 605 610cag cct cgt aac ctg gca aag tct gtc acc gtc gaa taaaaagatt 1985Gln Pro Arg Asn Leu Ala Lys Ser Val Thr Val Glu
615 620 625tcgcttctcg acg 1998<210>426<211>625<212>PRT<213>谷氨酸棒杆菌<400>426Met Arg Met Cys Gly Ile Val Gly Tyr Ile Gly Gln Ala Gly Asp Ser1 5 10 15Arg Asp Tyr Phe Ala Leu Asp Val Val Val Glu Gly Leu Arg Arg Leu
20 25 30Glu Tyr Arg Gly Tyr Asp Ser Ala Gly Ile Ala Ile His Ala Asn Gly
35 40 45Glu Ile Ser Tyr Arg Lys Lys Ala Gly Lys Val Ala Ala Leu Asp Ala
50 55 60Glu Ile Ala Lys Ala Pro Leu Pro Asp Ser Ile Leu Gly Ile Gly His65 70 75 80Thr Arg Trp Ala Thr His Gly Gly Pro Thr Asp Val Asn Ala His Pro
85 90 95His Val Val Ser Asn Gly Lys Leu Ala Val Val His Asn Gly Ile Ile
100 105 110Glu Asn Phe Ala Glu Leu Arg Ser Glu Leu Ser Ala Lys Gly Tyr Asn
115 120 125Phe Val Ser Asp Thr Asp Thr Glu Val Ala Ala Ser Leu Leu Ala Glu
130 135 140Ile Tyr Asn Thr Gln Ala Asn Gly Asp Leu Thr Leu Ala Met Gln Leu145 150 155 160Thr Gly Gln Arg Leu Glu Gly Ala Phe Thr Leu Leu Ala Ile His Ala
165 170 175Asp His Asp Asp Arg Ile Val Ala Ala Arg Arg Asn Ser Pro Leu Val
180 185 190Ile Gly Val Gly Glu Gly Glu Asn Phe Leu Gly Ser Asp Val Ser Gly
195 200 205Phe Ile Asp Tyr Thr Arg Lys Ala Val Glu Leu Ala Asn Asp Gln Val
210 215 220Val Thr Ile Thr Ala Asp Asp Tyr Ala Ile Thr Asn Phe Asp Gly Ser225 230 235 240Glu Ala Val Gly Lys Pro Phe Asp Val Glu Trp Asp Ala Ala Ala Ala
245 250 255Glu Lys Gly Gly Phe Gly Ser Phe Met Glu Lys Glu Ile His Asp Gln
260 265 270Pro Ala Ala Val Arg Asp Thr Leu Met Gly Arg Leu Asp Glu Asp Gly
275 280 285Lys Leu Val Leu Asp Glu Leu Arg Ile Asp Glu Ala Ile Leu Arg Ser
290 295 300Val Asp Lys Ile Val Ile Val Ala Cys Gly Thr Ala Ala Tyr Ala Gly305 310 315 320Gln Val Ala Arg Tyr Ala Ile Glu His Trp Cys Arg Ile Pro Thr Glu
325 330 335Val Glu Leu Ala His Glu Phe Arg Tyr Arg Asp Pro Ile Leu Asn Glu
340 345 350Lys Thr Leu Val Val Ala Leu Ser Gln Ser Gly Glu Thr Met Asp Thr
355 360 365Leu Met Ala Val Arg His Ala Arg Glu Gln Gly Ala Lys Val Val Ala
370 375 380Ile Cys Asn Thr Val Gly Ser Thr Leu Pro Arg Glu Ala Asp Ala Ser385 390 395 400Leu Tyr Thr Tyr Ala Gly Pro Glu Ile Ala Val Ala Ser Thr Lys Ala
405 410 415Phe Leu Ala Gln Ile Thr Ala Ser Tyr Leu Leu Gly Leu Tyr Leu Ala
420 425 430Gln Leu Arg Gly Asn Lys Phe Ala Asp Glu Val Ser Ser Ile Leu Asp
435 440 445Ser Leu Arg Glu Met Pro Glu Lys Ile Gln Gln Val Ile Asp Ala Glu
450 455 460Glu Gln Ile Lys Lys Leu Gly Gln Asp Met Ala Asp Ala Lys Ser Val465 470 475 480Leu Phe Leu Gly Arg His Val Gly Phe Pro Val Ala Leu Glu Gly Ala
485 490 495Leu Lys Leu Lys Glu Ile Ala Tyr Leu His Ala Glu Gly Phe Ala Ala
500 505 510Gly Glu Leu Lys His Gly Pro Ile Ala Leu Val Glu Glu Gly Gln Pro
515 520 525Ile Phe Val Ile Val Pro Ser Pro Arg Gly Arg Asp Ser Leu His Ser
530 535 540Lys Val Val Ser Asn Ile Gln Glu Ile Arg Ala Arg Gly Ala Val Thr545 550 555 560Ile Val Ile Ala Glu Glu Gly Asp Glu Ala Val Asn Asp Tyr Ala Asn
565 570 575Phe Ile Ile Arg Ile Pro Gln Ala Pro Thr Leu Met Gln Pro Leu Leu
580 585 590Ser Thr Val Pro Leu Gln Ile Phe Ala Cys Ala Val Ala Thr Ala Lys
595 600 605Gly Tyr Asn Val Asp Gln Pro Arg Asn Leu Ala Lys Ser Val Thr Val
610 615 620Glu625<210>427<211>666<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(666)<223>RXN03179<400>427gct cgt gag gca tgg cgc att ttc atg tcc cac tgg gat ctc tac gca 48Ala Arg Glu Ala Trp Arg Ile Phe Met Ser His Trp Asp Leu Tyr Ala1 5 10 15gga acc gca act ggc tac tgg gtg gag cag gaa ttt gag cac gtt ttc 96Gly Thr Ala Thr Gly Tyr Trp Val Glu Gln Glu Phe Glu His Val Phe
20 25 30ggc atc aac gcg gag cgc ctg aat gtt ggc acc cca gaa cat gct gac 144Gly Ile Asn Ala Glu Arg Leu Asn Val Gly Thr Pro Glu His Ala Asp
35 40 45gcc atc ttt gat gag ctg acc gat att ctt gcc aag cca gat ttc cga 192Ala Ile Phe Asp Glu Leu Thr Asp Ile Leu Ala Lys Pro Asp Phe Arg
50 55 60cca cgc gca ctg gct gag cag ttc aac ttg gaa gtt cta gcc acc acc 240Pro Arg Ala Leu Ala Glu Gln Phe Asn Leu Glu Val Leu Ala Thr Thr65 70 75 80gac gat ccg ctc gat gac ctg gca gat cac aag gca ctg gca gat gat 288Asp Asp Pro Leu Asp Asp Lan Ala Asp His Lys Ala Leu Ala Asp Asp
85 90 95cca acc ttc tcc cct cgt gtg ctc cct acc ttc cgc cca gac gca tac 336Pro Thr Phe Ser Pro Arg Val Leu Pro Thr Phe Arg Pro Asp Ala Tyr
100 105 110acc aag atg tac aac gct ggt tgg gca gaa aaa acc acc aag ctt atc 384Thr Lys Met Tyr Asn Ala Gly Trp Ala Glu Lys Thr Thr Lys Leu Ile
115 120 125gat acc gca ggt gac ggc aag gca ggc tgg gag ggt tac ctt cag gca 432Asp Thr Ala Gly Asp Gly Lys Ala Gly Trp Glu Gly Tyr Leu Gln Ala
130 135 140atg cgc aac cgc cgc cag tac ttc atc aat cac ggt gca acc tcc gcg 480Met Arg Asn Arg Arg Gln Tyr Phe Ile Asn His Gly Ala Thr Ser Ala145 150 155 160gac cac ggt ctc cac gac acc gac acc acc cca ctg agc cac aaa gat 528Asp His Gly Leu His Asp Thr Asp Thr Thr Pro Leu Ser His Lys Asp
165 170 175gcc cag aag atc ttg gac aag ggt ctc gct ggc aca gca acc ttg gct 576Ala Gln Lys Ile Leu Asp Lys Gly Leu Ala Gly Thr Ala Thr Leu Ala
180 185 190gaa atg cat gcc ttc gaa gcc aac acc acc tac cgt tcg cgg aaa tgt 624Glu Met His Ala Phe Glu Ala Asn Thr Thr Tyr Arg Ser Arg Lys Cys
195 200 205cca aga aga cgg ctg gtc atg acc atc cac cag gtg tgt acc 666Pro Arg Arg Arg Leu Val Met Thr Ile His Gln Val Cys Thr
210 215 220<210>428<211>222<212>PRT<213>谷氨酸棒杆菌<400>428Ala Arg Glu Ala Trp Arg Ile Phe Met Ser His Trp Asp Leu Tyr Ala1 5 10 15Gly Thr Ala Thr Gly Tyr Trp Val Glu Gln Glu Phe Glu His Val Phe
20 25 30Gly Ile Asn Ala Glu Arg Leu Asn Val Gly Thr Pro Glu His Ala Asp
35 40 45Ala Ile Phe Asp Glu Leu Thr Asp Ile Leu Ala Lys Pro Asp Phe Arg
50 55 60Pro Arg Ala Leu Ala Glu Gln Phe Asn Leu Glu Val Leu Ala Thr Thr65 70 75 80Asp Asp Pro Leu Asp Asp Leu Ala Asp His Lys Ala Leu Ala Asp Asp
85 90 95Pro Thr Phe Ser Pro Arg Val Leu Pro Thr Phe Arg Pro Asp Ala Tyr
100 105 110Thr Lys Met Tyr Asn Ala Gly Trp Ala Glu Lys Thr Thr Lys Leu Ile
115 120 125Asp Thr Ala Gly Asp Gly Lys Ala Gly Trp Glu Gly Tyr Leu Gln Ala
130 135 140Met Arg Asn Arg Arg Gln Tyr Phe Ile Asn His Gly Ala Thr Ser Ala145 150 155 160Asp His Gly Leu His Asp Thr Asp Thr Thr Pro Leu Ser His Lys Asp
165 170 175Ala Gln Lys Ile Leu Asp Lys Gly Leu Ala Gly Thr Ala Thr Leu Ala
180 185 190Glu Met His Ala Phe Glu Ala Asn Thr Thr Tyr Arg Ser Arg Lys Cys
195 200 205Pro Arg Arg Arg Leu Val Met Thr Ile His Gln Val Cys Thr
210 215 220<210>429<211>672<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(672)<223>FRXA02872<400>429gct cgt gag gca tgg cgc att ttc atg tcc cac tgg gat ctc tac gca 48Ala Arg Glu Ala Trp Arg Ile Phe Met Ser His Trp Asp Leu Tyr Ala1 5 10 15gga acc gca act ggc tac tgg gtg gag cag gaa ttt gag cac gtt ttc 96Gly Thr Ala Thr Gly Tyr Trp Val Glu Gln Glu Phe Glu His Val Phe
20 25 30ggc atc aac gcg gag cgc ctg aat gtt ggc acc cca gaa cat gct gac 144Gly Ile Asn Ala Glu Arg Leu Asn Val Gly Thr Pro Glu His Ala Asp
35 40 45gcc atc ttt gat gag ctg acc gat att ctt gcc aag cca gat ttc cga 192Ala Ile Phe Asp Glu Leu Thr Asp Ile Leu Ala Lys Pro Asp Phe Arg
50 55 60cca cgc gca ctg gct gag cag ttc aac ttg gaa gtt cta gcc acc acc 240Pro Arg Ala Leu Ala Glu Gln Phe Asn Leu Glu Val Leu Ala Thr Thr65 70 75 80gac gat ccg ctc gat gac ctg gca gat cac aag gca ctg gca gat gat 288Asp Asp Pro Leu Asp Asp Leu Ala Asp His Lys Ala Leu Ala Asp Asp
85 90 95cca acc ttc tcc cct cgt gtg ctc cct acc ttc cgc cca gac gca tac 336Pro Thr Phe Ser Pro Arg Val Leu Pro Thr Phe Arg Pro Asp Ala Tyr
100 105 110acc aag atg tac aac gct ggt tgg gca gaa aaa acc acc aag ctt atc 384Thr Lys Met Tyr Asn Ala Gly Trp Ala Glu Lys Thr Thr Lys Leu Ile
115 120 125gat acc gca ggt gac ggc aag gca ggc tgg gag ggt tac ctt cag gca 432Asp Thr Ala Gly Asp Gly Lys Ala Gly Trp Glu Gly Tyr Leu Gln Ala
130 135 140atg cgc aac cgc cgc cag tac ttc atc aat cac ggt gca acc tcc gcg 480Met Arg Asn Arg Arg Gln Tyr Phe Ile Asn His Gly Ala Thr Ser Ala145 150 155 160gac cac ggt ctc cac gac acc gac acc acc cca ctg agc cac aaa gat 528Asp His Gly Leu His Asp Thr Asp Thr Thr Pro Leu Ser His Lys Asp
165 170 175gcc cag aag atc ttg gac aag ggt ctc gct ggc aca gca acc ttg gct 576Ala Gln Lys Ile Leu Asp Lys Gly Leu Ala Gly Thr Ala Thr Leu Ala
180 185 190gaa atg cat gcc ttc gaa gcc aac acc acc tac cgt ttc gcg gaa atg 624Glu Met His Ala Phe Glu Ala Asn Thr Thr Tyr Arg Phe Ala Glu Met
195 200 205tcc caa gaa gac ggc ctg gtc atg acc atc cac cca ggt gtg tac cgc 672Ser Gln Glu Asp Gly Leu Val Met Thr Ile His Pro Gly Val Tyr Arg
210 215 220<210>430<211>224<212>PRT<213>谷氨酸棒杆菌<400>430Ala Arg Glu Ala Trp Arg Ile Phe Met Ser His Trp Asp Leu Tyr Ala1 5 10 15Gly Thr Ala Thr Gly Tyr Trp Val Glu Gln Glu Phe Glu His Val Phe
20 25 30Gly Ile Asn Ala Glu Arg Leu Asn Val Gly Thr Pro Glu His Ala Asp
35 40 45Ala Ile Phe Asp Glu Leu Thr Asp Ile Leu Ala Lys Pro Asp Phe Arg
50 55 60Pro Arg Ala Leu Ala Glu Gln Phe Asn Leu Glu Val Leu Ala Thr Thr65 70 75 80Asp Asp Pro Leu Asp Asp Leu Ala Asp His Lys Ala Leu Ala Asp Asp
85 90 95Pro Thr Phe Ser Pro Arg Val Leu Pro Thr Phe Arg Pro Asp Ala Tyr
100 105 110Thr Lys Met Tyr Asn Ala Gly Trp Ala Glu Lys Thr Thr Lys Leu Ile
115 120 125Asp Thr Ala Gly Asp Gly Lys Ala Gly Trp Glu Gly Tyr Leu Gln Ala
130 135 140Met Arg Asn Arg Arg Gln Tyr Phe Ile Asn His Gly Ala Thr Ser Ala145 150 155 160Asp His Gly Leu His Asp Thr Asp Thr Thr Pro Leu Ser His Lys Asp
165 170 175Ala Gln Lys Ile Leu Asp Lys Gly Leu Ala Gly Thr Ala Thr Leu Ala
180 185 190Glu Met His Ala Phe Glu Ala Asn Thr Thr Tyr Arg Phe Ala Glu Met
195 200 205Ser Gln Glu Asp Gly Leu Val Met Thr Ile His Pro Gly Val Tyr Arg
210 215 220<210>431<211>533<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(510)<223>RXN03180<400>431ttc ggt gag aac aaa gat ctc atc tct gac agc agt ttc aac cgc tgg 48Phe Gly Glu Asn Lys Asp Leu Ile Ser Asp Ser Ser Phe Asn Arg Trp1 5 10 15ctg cgt acg gtt tcc ctc gga tcg acc cag gat gcc gat atg gct gca 96Leu Arg Thr Val Ser Leu Gly Ser Thr Gln Asp Ala Asp Met Ala Ala
20 25 30gct tcc aac ttg gca gcc aat tct aaa atg gcc cgc cag aac acc cgc 144Ala Ser Asn Leu Ala Ala Asn Ser Lys Met Ala Arg Gln Asn Thr Arg
35 40 45gat atc ctc gac gca gtc tct gat ggt ggc gtc atg ctc ggc cga aac 192Asp Ile Leu Asp Ala Val Ser Asp Gly Gly Val Met Leu Gly Arg Asn
50 55 60ggt gcc cta gtg ttg gga cct gtg gtt gga act ctc cac att aaa ttc 240Gly Ala Leu Val Leu Gly Pro Val Val Gly Thr Leu His Ile Lys Phe65 70 75 80att gcg cct ttg aac aag cgt gtg gaa aga gtc atg tac aaa act gga 288Ile Ala Pro Leu Asn Lys Arg Val Glu Arg Val Met Tyr Lys Thr Gly
85 90 95ctc tca gaa gct gct gca gct gag caa tgt gct ttg gag gat cgt ctc 336Leu Ser Glu Ala Ala Ala Ala Glu Gln Cys Ala Leu Glu Asp Arg Leu
100 105 110cgc gaa gag atg gcc cac gct ttg tat caa tgg aat ccg gga cgc gat 384Arg Glu Glu Met Ala His Ala Leu Tyr Gln Trp Asn Pro Gly Arg Asp
115 120 125gaa aac tat gac ctc gtg atc aac acc ggt tcg atg aca tac gaa caa 432Glu Asn Tyr Asp Leu Val Ile Asn Thr Gly Ser Met Thr Tyr Glu Gln
130 135 140atc gtt gat cta gtt gtg gaa act tac gcc agg aag tat ccg ctc cac 480Ile Val Asp Leu Val Val Glu Thr Tyr Ala Arg Lys Tyr Pro Leu His145 150 155 160gtg aga atc att ccg aac gga aaa gac caa taaacataca gtccccgtga 530Val Arg Ile Ile Pro Asn Gly Lys Asp Gln
165 170tgt 533<210>432<211>170<212>PRT<213>谷氨酸棒杆菌<400>432Phe Gly Glu Asn Lys Asp Leu Ile Ser Asp Ser Ser Phe Asn Arg Trp1 5 10 15Leu Arg Thr Val Ser Leu Gly Ser Thr Gln Asp Ala Asp Met Ala Ala
20 25 30Ala Ser Asn Leu Ala Ala Asn Ser Lys Met Ala Arg Gln Asn Thr Arg
35 40 45Asp Ile Leu Asp Ala Val Ser Asp Gly Gly Val Met Leu Gly Arg Asn
50 55 60Gly Ala Leu Val Leu Gly Pro Val Val Gly Thr Leu His Ile Lys Phe65 70 75 80Ile Ala Pro Leu Asn Lys Arg Val Glu Arg Val Met Tyr Lys Thr Gly
85 90 95Leu Ser Glu Ala Ala Ala Ala Glu Gln Cys Ala Leu Glu Asp Arg Leu
100 105 110Arg Glu Glu Met Ala His Ala Leu Tyr Gln Trp Asn Pro Gly Arg Asp
115 120 125Glu Asn Tyr Asp Leu Val Ile Asn Thr Gly Ser Met Thr Tyr Glu Gln
130 135 140Ile Val Asp Leu Val Val Glu Thr Tyr Ala Arg Lys Tyr Pro Leu His145 150 155 160Val Arg Ile Ile Pro Asn Gly Lys Asp Gln
165 170<210>433<211>533<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(510)<223>FRXA02873<400>433ttc ggt gag aac aaa gat ctc atc tct gac agc agt ttc aac cgc tgg 48Phe Gly Glu Asn Lys Asp Leu Ile Ser Asp Ser Ser Phe Asn Arg Trp1 5 10 15ctg cgt acg gtt tcc ctc gga tcg acc cag gat gcc gat atg gct gca 96Leu Arg Thr Val Ser Leu Gly Ser Thr Gln Asp Ala Asp Met Ala Ala
20 25 30gct tcc aac ttg gca gcc aat tct aaa atg gcc cgc cag aac acc cgc 144Ala Ser Asn Leu Ala Ala Asn Ser Lys Met Ala Arg Gln Asn Thr Arg
35 40 45gat atc ctc gac gca gtc tct gat ggt ggc gtc atg ctc ggc cga aac 192Asp Ile Leu Asp Ala Val Ser Asp Gly Gly Val Met Leu Gly Arg Asn
50 55 60ggt gcc cta gtg ttg gga cct gtg gtt gga act ctc cac att aaa ttc 240Gly Ala Leu Val Leu Gly Pro Val Val Gly Thr Leu His Ile Lys Phe65 70 75 80att gcg cct ttg aac aag cgt gtg gaa aga gtc atg tac aaa act gga 288Ile Ala Pro Leu Asn Lys Arg Val Glu Arg Val Met Tyr Lys Thr Gly
85 90 95ctc tca gaa gct gct gca gct gag caa tgt gct ttg gag gat cgt ctc 336Leu Ser Glu Ala Ala Ala Ala Glu Gln Cys Ala Leu Glu Asp Arg Leu
100 105 110cgc gaa gag atg gcc cac gct ttg tat caa tgg aat ccg gga cgc gat 384Arg Glu Glu Met Ala His Ala Leu Tyr Gln Trp Asn Pro Gly Arg Asp
115 120 125gaa aac tat gac ctc gtg atc aac acc ggt tcg atg aca tac gaa caa 432Glu Asn Tyr Asp Leu Val Ile Asn Thr Gly Ser Met Thr Tyr Glu Gln
130 135 140atc gtt gat cta gtt gtg gaa act tac gcc agg aag tat ccg ctc cac 480Ile Val Asp Leu Val Val Glu Thr Tyr Ala Arg Lys Tyr Pro Leu His145 150 155 160gtg aga atc att ccg aac gga aaa gac caa taaacataca gtccccgtga 530Val Arg Ile Ile Pro Asn Gly Lys Asp Gln
165 170tgt 533<210>434<211>170<212>PRT<213>谷氨酸棒杆菌<400>434Phe Gly Glu Asn Lys Asp Leu Ile Ser Asp Ser Ser Phe Asn Arg Trp1 5 10 15Leu Arg Thr Val Ser Leu Gly Ser Thr Gln Asp Ala Asp Met Ala Ala
20 25 30Ala Ser Asn Leu Ala Ala Asn Ser Lys Met Ala Arg Gln Asn Thr Arg
35 40 45Asp Ile Leu Asp Ala Val Ser Asp Gly Gly Val Met Leu Gly Arg Asn
50 55 60Gly Ala Leu Val Leu Gly Pro Val Val Gly Thr Leu His Ile Lys Phe65 70 75 80Ile Ala Pro Leu Asn Lys Arg Val Glu Arg Val Met Tyr Lys Thr Gly
85 90 95Leu Ser Glu Ala Ala Ala Ala Glu Gln Cys Ala Leu Glu Asp Arg Leu
100 105 110Arg Glu Glu Met Ala His Ala Leu Tyr Gln Trp Asn Pro Gly Arg Asp
115 120 125Glu Asn Tyr Asp Leu Val Ile Asn Thr Gly Ser Met Thr Tyr Glu Gln
130 135 140Ile Val Asp Leu Val Val Glu Thr Tyr Ala Arg Lys Tyr Pro Leu His145 150 155 160Val Arg Ile Ile Pro Asn Gly Lys Asp Gln
165 170<210>435<211>798<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(775)<223>RXA02292<400>435tgcaccacgc cagctgcaac cctgcgcggt ggtctgggaa gttggtggag gggatcgtcg 60aaaagcgtag gcactaaagt tctcctgcac aatggaggat atg gac aat gac ttt 115
Met Asp Asn Asp Phe
1 5gaa tct atc gag aaa atg agc agc ggc gat tgg tac gtg gct acc ggc 163Glu Ser Ile Glu Lys Met Ser Ser Gly Asp Trp Tyr Val Ala Thr Gly
10 15 20gcg gaa cgt gaa gaa gtg gca caa aaa aca gcg tta ctt ttc cac gaa 211Ala Glu Arg Glu Glu Val Ala Gln Lys Thr Ala Leu Leu Phe His Glu
25 30 35tac aac caa att gga cct aca gac ccc gca cga act gcc gaa ata cta 259Tyr Asn Gln Ile Gly Pro Thr Asp Pro Ala Arg Thr Ala Glu Ile Leu
40 45 50aga act gta cta aat cct gcc agc gga acc tgc acg atc aaa gcg cca 307Arg Thr Val Leu Asn Pro Ala Ser Gly Thr Cys Thr Ile Lys Ala Pro
55 60 65gcc atc att gaa tac ggc ttc aac acc acg atc ggc gag cat gtg ttc 355Ala Ile Ile Glu Tyr Gly Phe Asn Thr Thr Ile Gly Glu His Val Phe70 75 80 85atc aac ttt ggc ctc acc att tta gat atc gca ccg gtt cgc atc ggg 403Ile Asn Phe Gly Leu Thr Ile Leu Asp Ile Ala Pro Val Arg Ile Gly
90 95 100gca cgc agc atg ctc ggg cca aac tgt cag ctc ttc acc gca ggt cac 451Ala Arg Ser Met Leu Gly Pro Asn Cys Gln Leu Phe Thr Ala Gly His
105 110 115ccg gtc gat gac tgg gaa atg cgc tcc ggt ggg tgg gaa aat ggc gca 499Pro Val Asp Asp Trp Glu Met Arg Ser Gly Gly Trp Glu Asn Gly Ala
120 125 130ccc att tcc att ggc gag gat acg tgg ctg ggt gga aat gtc acc gtc 547Pro Ile Ser Ile Gly Glu Asp Thr Trp Leu Gly Gly Asn Val Thr Val
135 140 145gtt ggt ggc gtg agc att ggc gat agg tgt gtg att ggc gcg ggg ccc 595Val Gly Gly Val Ser Ile Gly Asp Arg Cys Val Ile Gly Ala Gly Pro150 155 160 165gtg gtg acc aag gat att ccg gat gat tct att gct gtg ggc aac cct 643Val Val Thr Lys Asp Ile Pro Asp Asp Ser Ile Ala Val Gly Asn Pro
170 175 180gcg cga gta gtg cgg aaa cgt gat gat agc cgg ctc gaa cgt tcg cag 691Ala Arg Val Val Arg Lys Arg Asp Asp Ser Arg Leu Glu Arg Ser Gln
185 190 195ctg cca gaa ggt gct tcc gtg gat gcg ttg ggg att ctt cct aca aaa 739Leu Pro Glu Gly Ala Ser Val Asp Ala Leu Gly Ile Leu Pro Thr Lys
200 205 210tca cct agg ctg tca gaa aat att gcc gaa aaa tat taaatacgca 785Ser Pro Arg Leu Ser Glu Asn Ile Ala Glu Lys Tyr
215 220 225ggcactaaga aga 798<210>436<211>225<212>PRT<213>谷氨酸棒杆菌<400>436Met Asp Asn Asp Phe Glu Ser Ile Glu Lys Met Ser Ser Gly Asp Trp1 5 10 15Tyr Val Ala Thr Gly Ala Glu Arg Glu Glu Val Ala Gln Lys Thr Ala
20 25 30Leu Leu Phe His Glu Tyr Asn Gln Ile Gly Pro Thr Asp Pro Ala Arg
35 40 45Thr Ala Glu Ile Leu Arg Thr Val Leu Asn Pro Ala Ser Gly Thr Cys
50 55 60Thr Ile Lys Ala Pro Ala Ile Ile Glu Tyr Gly Phe Asn Thr Thr Ile65 70 75 80Gly Glu His Val Phe Ile Asn Phe Gly Leu Thr Ile Leu Asp Ile Ala
85 90 95Pro Val Arg Ile Gly Ala Arg Ser Met Leu Gly Pro Asn Cys Gln Leu
100 105 110Phe Thr Ala Gly His Pro Val Asp Asp Trp Glu Met Arg Ser Gly Gly
115 120 125Trp Glu Asn Gly Ala Pro Ile Ser Ile Gly Glu Asp Thr Trp Leu Gly
130 135 140Gly Asn Val Thr Val Val Gly Gly Val Ser Ile Gly Asp Arg Cys Val145 150 155 160Ile Gly Ala Gly Pro Val Val Thr Lys Asp Ile Pro Asp Asp Ser Ile
165 170 175Ala Val Gly Asn Pro Ala Arg Val Val Arg Lys Arg Asp Asp Ser Arg
180 185 190Leu Glu Arg Ser Gln Leu Pro Glu Gly Ala Ser Val Asp Ala Leu Gly
195 200 205Ile Leu Pro Thr Lys Ser Pro Arg Leu Ser Glu Asn Ile Ala Glu Lys
210 215 220Tyr225<210>437<211>891<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(868)<223>RXA02666<400>437gctcggcgac gaggaagaga agaaggacgc attcgacgac ttcgacgatt ccgacgtgga 60tcttgacgat ctgagcttcg acgacgaaga ttagacgccc atg tcg tct aca cga 115
Met Ser Ser Thr Arg
1 5atc ccc gtc atc gca ctc ctc gcg gcg gcg ggg cgc gga acc cgc ctc 163Ile Pro Val Ile Ala Leu Leu Ala Ala Ala Gly Arg Gly Thr Arg Leu
10 15 20ggc gga ccc atc ccc aaa gca ttc gtc acg ttg cgt gaa cgc aca ctt 211Gly Gly Pro Ile Pro Lys Ala Phe Val Thr Leu Arg Glu Arg Thr Leu
25 30 35tta gag cgc tcg ctc caa gcc atg ctc acc tcc gaa agc gtc gac gaa 259Leu Glu Arg Ser Leu Gln Ala Met Leu Thr Ser Glu Ser Val Asp Glu
40 45 50atc atc atc ctc gtc agc ccc gac atg gaa acc tac gcc cgc gat ttg 307Ile Ile Ile Leu Val Ser Pro Asp Met Glu Thr Tyr Ala Arg Asp Leu
55 60 65ctg cgc aaa cgc ggt ctt ttg aac gac ccc gaa ggg gta cgc gta cgg 355Leu Arg Lys Arg Gly Leu Leu Asn Asp Pro Glu Gly Val Arg Val Arg70 75 80 85ctc gtg cac ggc ggc ggg gag cgc gcg gac tcg gtc tgg gca ggc ctt 403Leu Val His Gly Gly Gly Glu Arg Ala Asp Ser Val Trp Ala Gly Leu
90 95 100cag gca att tcg ctt gac gac gcc acc ccc gat gca att gtc tta atc 451Gln Ala Ile Ser Leu Asp Asp Ala Thr Pro Asp Ala Ile Val Leu Ile
105 110 115cac gac agc gcc cga gcg ctc aca cca ccc ggc atg att gcg cgc gtg 499His Asp Ser Ala Arg Ala Leu Thr Pro Pro Gly Met Ile Ala Arg Val
120 125 130gtg cgc aaa gtc cac gaa ggc gcc acc gca gtc atc cca gta ctg cca 547Val Arg Lys Val His Glu Gly Ala Thr Ala Val Ile Pro Val Leu Pro
135 140 145gta tcg gac acc atc aaa cga gtg tcc cct gat ggc gga gta gtt gtc 595Val Ser Asp Thr Ile Lys Arg Val Ser Pro Asp Gly Gly Val Val Val150 155 160 165gac aca ccc aac cgt gca gaa ctt cgc gcc gtc caa acc cca caa ggc 643Asp Thr Pro Asn Arg Ala Glu Leu Arg Ala Val Gln Thr Pro Gln Gly
170 175 180ttc ctg ctg tcc gaa ctt gtt gca gcg aat gag aaa ttc ttc gcc gac 691Phe Leu Leu Ser Glu Leu Val Ala Ala Asn Glu Lys Phe Phe Ala Asp
185 190 195ccc aac cca ggc ttc atc cca acc gat gac gcc agc ttg atg gaa tgg 739Pro Asn Pro Gly Phe Ile Pro Thr Asp Asp Ala Ser Leu Met Glu Trp
200 205 210tac ggc gca gat gta gtc tgc gta caa ggc gac cca atg gcg ttt aaa 787Tyr Gly Ala Asp Val Val Cys Val Gln Gly Asp Pro Met Ala Phe Lys
215 220 225gta aca acc ccc att gat atg atg ctg gca caa cgc atc acc gac gaa 835Val Thr Thr Pro Ile Asp Met Met Leu Ala Gln Arg Ile Thr Asp Glu230 235 240 245gcc gaa ccc aca ata ttt gag gta cca ggt gac taacccaatc atcccccgcg 888Ala Glu Pro Thr Ile Phe Glu Val Pro Gly Asp
250 255tag 891<210>438<211>256<212>PRT<213>谷氨酸棒杆菌<400>438Met Ser Ser Thr Arg Ile Pro Val Ile Ala Leu Leu Ala Ala Ala Gly1 5 10 15Arg Gly Thr Arg Leu Gly Gly Pro Ile Pro Lys Ala Phe Val Thr Leu
20 25 30Arg Glu Arg Thr Leu Leu Glu Arg Ser Leu Gln Ala Met Leu Thr Ser
35 40 45Glu Ser Val Asp Glu Ile Ile Ile Leu Val Ser Pro Asp Met Glu Thr
50 55 60Tyr Ala Arg Asp Leu Leu Arg Lys Arg Gly Leu Leu Asn Asp Pro Glu65 70 75 80Gly Val Arg Val Arg Leu Val His Gly Gly Gly Glu Arg Ala Asp Ser
85 90 95Val Trp Ala Gly Leu Gln Ala Ile Ser Leu Asp Asp Ala Thr Pro Asp
100 105 110Ala Ile Val Leu Ile His Asp Ser Ala Arg Ala Leu Thr Pro Pro Gly
115 120 125Met Ile Ala Arg Val Val Arg Lys Val His Glu Gly Ala Thr Ala Val
130 135 140Ile Pro Val Leu Pro Val Ser Asp Thr Ile Lys Arg Val Ser Pro Asp145 150 155 160Gly Gly Val Val Val Asp Thr Pro Asn Arg Ala Glu Leu Arg Ala Val
165 170 175Gln Thr Pro Gln Gly Phe Leu Leu Ser Glu Leu Val Ala Ala Asn Glu
180 185 190Lys Phe Phe Ala Asp Pro Asn Pro Gly Phe Ile Pro Thr Asp Asp Ala
195 200 205Ser Leu Met Glu Trp Tyr Gly Ala Asp Val Val Cys Val Gln Gly Asp
210 215 220Pro Met Ala Phe Lys Val Thr Thr Pro Ile Asp Met Met Leu Ala Gln225 230 235 240Arg Ile Thr Asp Glu Ala Glu Pro Thr Ile Phe Glu Val Pro Gly Asp
245 250 255<210>439<211>1065<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1042)<223>RXA00202<400>439ctggcagcag attgtcatcg gttgtgtcat cgcgcttgcg gtgggcttcg atgtcatccg 60aaacaaaacc tctaagtaat tcctgaaagg aaattttcac atg tac gct cgt aaa 115
Met Tyr Ala Arg Lys
1 5ctt att gct ctg tcc gct tct gtc gtt ttg gct ttc agc ttg tct gct 163Leu Ile Ala Leu Ser Ala Ser Val Val Leu Ala Phe Ser Leu Ser Ala
10 15 20tgc aac cgt gaa tct tct ggc acc agc gca gac ggc ggt tct gcg gat 211Cys Asn Arg Glu Ser Ser Gly Thr Ser Ala Asp Gly Gly Ser Ala Asp
25 30 35ggg tcg atc acc ttg gct ctg tct acc cag acc aac ccg ttc ttt gtg 259Gly Ser Ile Thr Leu Ala Leu Ser Thr Gln Thr Asn Pro Phe Phe Val
40 45 50cag ctt cgt gat ggt gcc cag gaa aag gct gat gaa ttg ggc gtg acc 307Gln Leu Arg Asp Gly Ala Gln Glu Lys Ala Asp Glu Leu Gly Val Thr
55 60 65ctc aat gtt cag gat gct tcc gat gac gct gca acg cag gcc aac cag 355Leu Asn Val Gln Asp Ala Ser Asp Asp Ala Ala Thr Gln Ala Asn Gln70 75 80 85ctc aac aac gct gtc acc acc ggt gct ggc gtg gtg att gtc aac cca 403Leu Asn Asn Ala Val Thr Thr Gly Ala Gly Val Val Ile Val Asn Pro
90 95 100act gat tct gat gct gtg gtg ccg tcg gtg gaa gct ctc aac cag gct 451Thr Asp Ser Asp Ala Val Val Pro Ser Val Glu Ala Leu Asn Gln Ala
105 110 115gac att cct gtt gtg gct gtc gac cgt tcc tcc aat ggt ggc gag gtg 499Asp Ile Pro Val Val Ala Val Asp Arg Ser Ser Asn Gly Gly Glu Val
120 125 130gcg tcc ttc gtg gca tct gac aac gtt gct ggc ggc gcg cag gct gct 547Ala Ser Phe Val Ala Ser Asp Asn Val Ala Gly Gly Ala Gln Ala Ala
135 140 145gca gcc ctg gca gag gcg atc ggt ggc gaa ggt gaa atc ctc atg ctg 595Ala Ala Leu Ala Glu Ala Ile Gly Gly Glu Gly Glu Ile Leu Met Leu150 155 160 165caa ggc att gcg gga tcc tct gca tca cgt gat cgt gga cag gga ttt 643Gln Gly Ile Ala Gly Ser Ser Ala Ser Arg Asp Arg Gly Gln Gly Phe
170 175 180gaa gag gag atc gct aag cat gag ggc att tcc att gtg gct aag cag 691Glu Glu Glu Ile Ala Lys His Glu Gly Ile Ser Ile Val Ala Lys Gln
185 190 195acc gcc aac ttt gac cgc ggt gag ggc ctg gac gtg gca act aac ctg 739Thr Ala Asn Phe Asp Arg Gly Glu Gly Leu Asp Val Ala Thr Asn Leu
200 205 210ctg cag gca cac ccc aat gtg aag gcg atc ttc gcg gaa aac gat gag 787Leu Gln Ala His Pro Asn Val Lys Ala Ile Phe Ala Glu Asn Asp Glu
215 220 225atg gcg ttg ggc gca atc gaa gcc ctg ggt gct cgt gct ggt gaa gat 835Met Ala Leu Gly Ala Ile Glu Ala Leu Gly Ala Arg Ala Gly Glu Asp230 235 240 245gtc atc gtt gtc ggt ttc gat ggc acc aat gat ggt ctg gca gcg gtt 883Val Ile Val Val Gly Phe Asp Gly Thr Asn Asp Gly Leu Ala Ala Val
250 255 260gaa gat gga cgc atg ttg gcc acc gtt gct cag cag cca gaa gag ctg 931Glu Asp Gly Arg Met Leu Ala Thr Val Ala Gln Gln Pro Glu Glu Leu
265 270 275gga gca aag gct gtg gaa gaa gca gct aag ctc ctg cgc ggt gag gac 979Gly Ala Lys Ala Val Glu Glu Ala Ala Lys Leu Leu Arg Gly Glu Asp
280 285 290gct gaa aca gag gta cca gtt gag gtt gtc act gtg aag ctc gac aac 1027Ala Glu Thr Glu Val Pro Val Glu Val Val Thr Val Lys Leu Asp Asn
295 300 305gtc gcg gac ttc aag tagtcggcga tgaaaaagtc cgt 1065Val Ala Asp Phe Lys310<210>440<211>314<212>PRT<213>谷氨酸棒杆菌<400>440Met Tyr Ala Arg Lys Leu Ile Ala Leu Ser Ala Ser Val Val Leu Ala1 5 10 15Phe Ser Leu Ser Ala Cys Asn Arg Glu Ser Ser Gly Thr Ser Ala Asp
20 25 30Gly Gly Ser Ala Asp Gly Ser Ile Thr Leu Ala Leu Ser Thr Gln Thr
35 40 45Asn Pro Phe Phe Val Gln Leu Arg Asp Gly Ala Gln Glu Lys Ala Asp
50 55 60Glu Leu Gly Val Thr Leu Asn Val Gln Asp Ala Ser Asp Asp Ala Ala65 70 75 80Thr Gln Ala Asn Gln Leu Asn Asn Ala Val Thr Thr Gly Ala Gly Val
85 90 95Val Ile Val Asn Pro Thr Asp Ser Asp Ala Val Val Pro Ser Val Glu
100 105 110Ala Leu Asn Gln Ala Asp Ile Pro Val Val Ala Val Asp Arg Ser Ser
115 120 125Asn Gly Gly Glu Val Ala Ser Phe Val Ala Ser Asp Asn Val Ala Gly
130 135 140Gly Ala Gln Ala Ala Ala Ala Leu Ala Glu Ala Ile Gly Gly Glu Gly145 150 155 160Glu Ile Leu Met Leu Gln Gly Ile Ala Gly Ser Ser Ala Ser Arg Asp
165 170 175Arg Gly Gln Gly Phe Glu Glu Glu Ile Ala Lys His Glu Gly Ile Ser
180 185 190Ile Val Ala Lys Gln Thr Ala Asn Phe Asp Arg Gly Glu Gly Leu Asp
195 200 205Val Ala Thr Asn Leu Leu Gln Ala His Pro Asn Val Lys Ala Ile Phe
210 215 220Ala Glu Asn Asp Glu Met Ala Leu Gly Ala Ile Glu Ala Leu Gly Ala225 230 235 240Arg Ala Gly Glu Asp Val Ile Val Val Gly Phe Asp Gly Thr Asn Asp
245 250 255Gly Leu Ala Ala Val Glu Asp Gly Arg Met Leu Ala Thr Val Ala Gln
260 265 270Gln Pro Glu Glu Leu Gly Ala Lys Ala Val Glu Glu Ala Ala Lys Leu
275 280 285Leu Arg Gly Glu Asp Ala Glu Thr Glu Val Pro Val Glu Val Val Thr
290 295 300Val Lys Leu Asp Asn Val Ala Asp Phe Lys305 310<210>441<211>963<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(940)<223>RXA02440<400>441gctgttaatc acgggttgtt cagctaccgg gggagcacca cgggcaacag atggggcatc 60aggcggagga accgtcgata cgcctcggtt ggttgtcgcg atg gtg agc cac ggc 115
Met Val Ser His Gly
1 5gcg ccg ggc gat act ttt tgg gat ttg gtc cga aaa ggt gct gaa gac 163Ala Pro Gly Asp Thr Phe Trp Asp Leu Val Arg Lys Gly Ala Glu Asp
10 15 20gcc gcc caa aaa gac aac gtt gaa ctc cgc tat tcc tct aat ccg gaa 211Ala Ala Gln Lys Asp Asn Val Glu Leu Arg Tyr Ser Ser Asn Pro Glu
25 30 35atc cct gaa caa tcc aac ctc gtg caa aat gcc atc gat tca cgc gtc 259Ile Pro Glu Gln Ser Asn Leu Val Gln Asn Ala Ile Asp Ser Arg Val
40 45 50gac ggc atc gcc atg acc atg cct aat gct caa tca cta gga ccg gtc 307Asp Gly Ile Ala Met Thr Met Pro Asn Ala Gln Ser Leu Gly Pro Val
55 60 65gct caa aag gcc gtg gat gcg ggc att cct gtg gtt ggt ctc aac gct 355Ala Gln Lys Ala Val Asp Ala Gly Ile Pro Val Val Gly Leu Asn Ala70 75 80 85gga atg aac gaa tac caa gat tat gga atg aca gga ttc ttt ggt caa 403Gly Met Asn Glu Tyr Gln Asp Tyr Gly Met Thr Gly Phe Phe Gly Gln
90 95 100gat gaa tcc gtc gca gga gca tcc gca gga gcg cgc ctt gcc gag gaa 451Asp Glu Ser Val Ala Gly Ala Ser Ala Gly Ala Arg Leu Ala Glu Glu
105 110 115aac gca caa aaa gtt ttg tgt gtg atc cat gaa cag ggc aac tcc tcc 499Asn Ala Gln Lys Val Leu Cys Val Ile His Glu Gln Gly Asn Ser Ser
120 125 130cag gaa gct cgc tgt ggt ggc gtg tct gaa ggt ttg ggc aaa caa gta 547Gln Glu Ala Arg Cys Gly Gly Val Ser Glu Gly Leu Gly Lys Gln Val
135 140 145gaa acc ctg tat gtc aac ggc atg gat ctc acc tca gtg aac tcc acc 595Glu Thr Leu Tyr Val Asn Gly Met Asp Leu Thr Ser Val Asn Ser Thr150 155 160 165ctg cag gca aaa ctt gct caa gac cgc agc att gat tgg gtt gtg gga 643Leu Gln Ala Lys Leu Ala Gln Asp Arg Ser Ile Asp Trp Val Val Gly
170 175 180ctc cag gct ggt gta tca atg gct att tct gat gcg gca gac gct gcg 691Leu Gln Ala Gly Val Ser Met Ala Ile Ser Asp Ala Ala Asp Ala Ala
185 190 195aac tca gaa gta aag atc gcc acc ttt gat aca aac gca cag ctc atg 739Asn Ser Glu Val Lys Ile Ala Thr Phe Asp Thr Asn Ala Gln Leu Met
200 205 210acc gct att cgt gat ggc aag atc caa ttc gcc att gat cag caa cca 787Thr Ala Ile Arg Asp Gly Lys Ile Gln Phe Ala Ile Asp Gln Gln Pro
215 220 225tat ctg cag ggc tac atg gcc gtg gat tcg ctg tgg ttg gcg cac cga 835Tyr Leu Gln Gly Tyr Met Ala Val Asp Ser Leu Trp Leu Ala His Arg230 235 240 245aac ggc acc act gtt ggt ggc gga cga ccc gtg tac aca gga cca gcc 883Asn Gly Thr Thr Val Gly Gly Gly Arg Pro Val Tyr Thr Gly Pro Ala
250 255 260att gtg gat gcc acc aac gtt gat gtc att gct gaa gcc gtt ggg gag 931Ile Val Asp Ala Thr Asn Val Asp Val Ile Ala Glu Ala Val Gly Glu
265 270 275ggt ctg cga tgacaaaaat caagagtggg gag 963Gly Leu Arg
280<210>442<211>280<212>PRT<213>谷氨酸棒杆菌<400>442Met Val Ser His Gly Ala Pro Gly Asp Thr Phe Trp Asp Leu Val Arg1 5 10 15Lys Gly Ala Glu Asp Ala Ala Gln Lys Asp Asn Val Glu Leu Arg Tyr
20 25 30Ser Ser Asn Pro Glu Ile Pro Glu Gln Ser Asn Leu Val Gln Asn Ala
35 40 45Ile Asp Ser Arg Val Asp Gly Ile Ala Met Thr Met Pro Asn Ala Gln
50 55 60Ser Leu Gly Pro Val Ala Gln Lys Ala Val Asp Ala Gly Ile Pro Val65 70 75 80Val Gly Leu Asn Ala Gly Met Asn Glu Tyr Gln Asp Tyr Gly Met Thr
85 90 95Gly Phe Phe Gly Gln Asp Glu Ser Val Ala Gly Ala Ser Ala Gly Ala
100 105 110Arg Leu Ala Glu Glu Asn Ala Gln Lys Val Leu Cys Val Ile His Glu
115 120 125Gln Gly Asn Ser Ser Gln Glu Ala Arg Cys Gly Gly Val Ser Glu Gly
130 135 140Leu Gly Lys Gln Val Glu Thr Leu Tyr Val Asn Gly Met Asp Leu Thr145 150 155 160Ser Val Asn Ser Thr Leu Gln Ala Lys Leu Ala Gln Asp Arg Ser Ile
165 170 175Asp Trp Val Val Gly Leu Gln Ala Gly Val Ser Met Ala Ile Ser Asp
180 185 190Ala Ala Asp Ala Ala Asn Ser Glu Val Lys Ile Ala Thr Phe Asp Thr
195 200 205Asn Ala Gln Leu Met Thr Ala Ile Arg Asp Gly Lys Ile Gln Phe Ala
210 215 220Ile Asp Gln Gln Pro Tyr Leu Gln Gly Tyr Met Ala Val Asp Ser Leu225 230 235 240Trp Leu Ala His Arg Asn Gly Thr Thr Val Gly Gly Gly Arg Pro Val
245 250 255Tyr Thr Gly Pro Ala Ile Val Asp Ala Thr Asn Val Asp Val Ile Ala
260 265 270Glu Ala Val Gly Glu Gly Leu Arg
275 280<210>443<211>1482<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1459)<223>RXN01569<400>443aaggcctaga gcagaccatc gattggtacc gcgaaaacga ggcctggtgg cgccctgcca 60agaacaacgt cgaagctacc tacgctaagc agggacaata atg gaa tac ggt aaa 115
Met Glu Tyr Gly Lys
1 5caa ctc acc tcc cac acc acc gac atc gaa ggc cta ctg gtt ttc gat 163Gln Leu Thr Ser His Thr Thr Asp Ile Glu Gly Leu Leu Val Phe Asp
10 15 20ttc ccc gtc cac ggc gac aac cgc ggc tgg ttc aag gaa aat tgg cag 211Phe Pro Val His Gly Asp Asn Arg Gly Trp Phe Lys Glu Asn Trp Gln
25 30 35cgc acc aag atg acc aac ctg ggg ctg ccc gat ttt ggc ccc gtc caa 259Arg Thr Lys Met Thr Asn Leu Gly Leu Pro Asp Phe Gly Pro Val Gln
40 45 50aac aac atg agt ttc aac gcc acc gcc ggc acg act cgc ggc atg cac 307Asn Asn Met Ser Phe Asn Ala Thr Ala Gly Thr Thr Arg Gly Met His
55 60 65gct gag ccg tgg gat aaa ttt gtg tcc gtc gcg gtg ggt tcc gtt ttc 355Ala Glu Pro Trp Asp Lys Phe Val Ser Val Ala Val Gly Ser Val Phe70 75 80 85gga gct tgg gtg gat ctg cgc gcg ggc tcg agc acg tac ggt aac gtc 403Gly Ala Trp Val Asp Leu Arg Ala Gly Ser Ser Thr Tyr Gly Asn Val
90 95 100gta acg caa aaa att acc cct gac gtg gga gtt tac gtc ccg cgt ggt 451Val Thr Gln Lys Ile Thr Pro Asp Val Gly Val Tyr Val Pro Arg Gly
105 110 115gtg gca aac ggc ttc cag gcg ctc gag gac ggc acg ctg tac acc tac 499Val Ala Asn Gly Phe Gln Ala Leu Glu Asp Gly Thr Leu Tyr Thr Tyr
120 125 130ctc gtc aac gat cat tgg tcc ccc gac gcg cat tac gcc aac gtc aac 547Leu Val Asn Asp His Trp Ser Pro Asp Ala His Tyr Ala Asn Val Asn
135 140 145ctc aac atg atc gac tgg ccg ctg ccc atc acc gag atc tcc gaa aaa 595Leu Asn Met Ile Asp Trp Pro Leu Pro Ile Thr Glu Ile Ser Glu Lys150 155 160 165gat aaa aaa cat cca gcg ctt atc gac gcc acc ccc ctg ccc gcc cgc 643Asp Lys Lys His Pro Ala Leu Ile Asp Ala Thr Pro Leu Pro Ala Arg
170 175 180aag gtt ctc gtg gtc ggc gcc ggc gga caa ctg gga acc gcg cta cgc 691Lys Val Leu Val Val Gly Ala Gly Gly Gln Leu Gly Thr Ala Leu Arg
185 190 195gcg cag ttc cca gac gcg gaa ttt gtc acg cgc caa gaa ctc gat atc 739Ala Gln Phe Pro Asp Ala Glu Phe Val Thr Arg Gln Glu Leu Asp Ile
200 205 210acc tca gat ctc acc gag gct cgc gcg tgg aaa caa tac tcc acc atc 787Thr Ser Asp Leu Thr Glu Ala Arg Ala Trp Lys Gln Tyr Ser Thr Ile
215 220 225ata aac gcc gcc gcc tac act gcc gtt gac cag gca gaa cac gac cgc 835Ile Asn Ala Ala Ala Tyr Thr Ala Val Asp Gln Ala Glu His Asp Arg230 235 240 245gca gca gcg tgg gac atc aac gca gcg gca gtg gct aac ctc gcg acc 883Ala Ala Ala Trp Asp Ile Asn Ala Ala Ala Val Ala Asn Leu Ala Thr
250 255 260atc gcg cgc gac aac aac ctc acc ctc gtg cac gtg tcc tca gat tat 931Ile Ala Arg Asp Asn Asn Leu Thr Leu Val His Val Ser Ser Asp Tyr
265 270 275gtc ttc gac ggt gcg gcc gaa tcc tac gat gaa aac gca ccg ttt tcc 979Val Phe Asp Gly Ala Ala Glu Ser Tyr Asp Glu Asn Ala Pro Phe Ser
280 285 290cca ctc ggc gtg tac ggc caa tcc aaa gca gcc ggc gac atc gga gac 1027Pro Leu Gly Val Tyr Gly Gln Ser Lys Ala Ala Gly Asp Ile Gly Asp
295 300 305acc acc gca ccg cgc cac tac att gtg cgc acc agc tgg gtg att ggc 1075Thr Thr Ala Pro Arg His Tyr Ile Val Arg Thr Ser Trp Val Ile Gly310 315 320 325gat ggc aat aat ttt gtc cgc acc atg aaa tcc ctc gac gaa cgc ggc 1123Asp Gly Asn Asn Phe Val Arg Thr Met Lys Ser Leu Asp Glu Arg Gly
330 335 340atc gca cca tca gta gtt gat gat caa atc ggc cgc cta tcc ttc acc 1171Ile Ala Pro Ser Val Val Asp Asp Gln Ile Gly Arg Leu Ser Phe Thr
345 350 355gaa gac atc gca gcc ggc atc gcg cac ctt ttg gaa gtg ggt gca gca 1219Glu Asp Ile Ala Ala Gly Ile Ala His Leu Leu Glu Val Gly Ala Ala
360 365 370tat ggc acc tac aac ctc acc aac acc ggc gaa ccc gca agc tgg gcc 1267Tyr Gly Thr Tyr Asn Leu Thr Asn Thr Gly Glu Pro Ala Ser Trp Ala
375 380 385gat gtt gcc cgc gca gta ttt tcc gac ccc acc aaa gtt acc ggc gtg 1315Asp Val Ala Arg Ala Val Phe Ser Asp Pro Thr Lys Val Thr Gly Val390 395 400 405agc acc gcc gag tac ttc gcc aac aaa gac gca gcg ccc cgc cca ctg 1363Ser Thr Ala Glu Tyr Phe Ala Asn Lys Asp Ala Ala Pro Arg Pro Leu
410 415 420aac tcc gtt ttg gat ctc ggc aaa atc gaa gcc acc gga ttt agc gca 1411Asn Ser Val Leu Asp Leu Gly Lys Ile Glu Ala Thr Gly Phe Ser Ala
425 430 435ccg acc tgg cag acc cgc ctc aac gac tac ctc aag gaa ctc tca aag 1459Pro Thr Trp Gln Thr Arg Leu Asn Asp Tyr Leu Lys Glu Leu Ser Lys
440 445 450tgaaaggcat catcctcgca ggt 1482<210>444<211>453<212>PRT<213>谷氨酸棒杆菌<400>444Met Glu Tyr Gly Lys Gln Leu Thr Ser His Thr Thr Asp Ile Glu Gly1 5 10 15Leu Leu Val Phe Asp Phe Pro Val His Gly Asp Asn Arg Gly Trp Phe
20 25 30Lys Glu Asn Trp Gln Arg Thr Lys Met Thr Asn Leu Gly Leu Pro Asp
35 40 45Phe Gly Pro Val Gln Asn Asn Met Ser Phe Asn Ala Thr Ala Gly Thr
50 55 60Thr Arg Gly Met His Ala Glu Pro Trp Asp Lys Phe Val Ser Val Ala65 70 75 80Val Gly Ser Val Phe Gly Ala Trp Val Asp Leu Arg Ala Gly Ser Ser
85 90 95Thr Tyr Gly Asn Val Val Thr Gln Lys Ile Thr Pro Asp Val Gly Val
100 105 110Tyr Val Pro Arg Gly Val Ala Asn Gly Phe Gln Ala Leu Glu Asp Gly
115 120 125Thr Leu Tyr Thr Tyr Leu Val Asn Asp His Trp Ser Pro Asp Ala His
130 135 140Tyr Ala Asn Val Asn Leu Asn Met Ile Asp Trp Pro Leu Pro Ile Thr145 150 155 160Glu Ile Ser Glu Lys Asp Lys Lys His Pro Ala Leu Ile Asp Ala Thr
165 170 175Pro Leu Pro Ala Arg Lys Val Leu Val Val Gly Ala Gly Gly Gln Leu
180 185 190Gly Thr Ala Leu Arg Ala Gln Phe Pro Asp Ala Glu Phe Val Thr Arg
195 200 205Gln Glu Leu Asp Ile Thr Ser Asp Leu Thr Glu Ala Arg Ala Trp Lys
210 215 220Gln Tyr Ser Thr Ile Ile Asn Ala Ala Ala Tyr Thr Ala Val Asp Gln225 230 235 240Ala Glu His Asp Arg Ala Ala Ala Trp Asp Ile Asn Ala Ala Ala Val
245 250 255Ala Asn Leu Ala Thr Ile Ala Arg Asp Asn Asn Leu Thr Leu Val His
260 265 270Val Ser Ser Asp Tyr Val Phe Asp Gly Ala Ala Glu Ser Tyr Asp Glu
275 280 285Asn Ala Pro Phe Ser Pro Leu Gly Val Tyr Gly Gln Ser Lys Ala Ala
290 295 300Gly Asp Ile Gly Asp Thr Thr Ala Pro Arg His Tyr Ile Val Arg Thr305 310 315 320Ser Trp Val Ile Gly Asp Gly Asn Asn Phe Val Arg Thr Met Lys Ser
325 330 335Leu Asp Glu Arg Gly Ile Ala Pro Ser Val Val Asp Asp Gln Ile Gly
340 345 350Arg Leu Ser Phe Thr Glu Asp Ile Ala Ala Gly Ile Ala His Leu Leu
355 360 365Glu Val Gly Ala Ala Tyr Gly Thr Tyr Asn Leu Thr Asn Thr Gly Glu
370 375 380Pro Ala Ser Trp Ala Asp Val Ala Arg Ala Val Phe Ser Asp Pro Thr385 390 395 400Lys Val Thr Gly Val Ser Thr Ala Glu Tyr Phe Ala Asn Lys Asp Ala
405 410 415Ala Pro Arg Pro Leu Asn Ser Val Leu Asp Leu Gly Lys Ile Glu Ala
420 425 430Thr Gly Phe Ser Ala Pro Thr Trp Gln Thr Arg Leu Asn Asp Tyr Leu
435 440 445Lys Glu Leu Ser Lys
450<210>445<211>449<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(426)<223>FRXA01569<400>445gca ccg cgc cac tac att gtg cgc acc agc tgg gtg att ggc gat ggc 48Ala Pro Arg His Tyr Ile Val Arg Thr Ser Trp Val Ile Gly Asp Gly1 5 10 15aat aat ttt gtc cgc acc atg aaa tcc ctc gac gaa cgc ggc atc gca 96Asn Asn Phe Val Arg Thr Met Lys Ser Leu Asp Glu Arg Gly Ile Ala
20 25 30cca tca gta gtt gat gat caa atc ggc cgc cta tcc ttc acc gaa gac 144Pro Ser Val Val Asp Asp Gln Ile Gly Arg Leu Ser Phe Thr Glu Asp
35 40 45atc gca gcc ggc atc gcg cac ctt ttg gaa gtg ggt gca gca tat ggc 192Ile Ala Ala Gly Ile Ala His Leu Leu Glu Val Gly Ala Ala Tyr Gly
50 55 60acc tac aac ctc acc aac acc ggc gaa ccc gca agc tgg gcc gat gtt 240Thr Tyr Asn Leu Thr Asn Thr Gly Glu Pro Ala Ser Trp Ala Asp Val65 70 75 80gcc cgc gca gta ttt tcc gac ccc acc aaa gtt acc ggc gtg agc acc 288Ala Arg Ala Val Phe Ser Asp Pro Thr Lys Val Thr Gly Val Ser Thr
85 90 95gcc gag tac ttc gcc aac aaa gac gca gcg ccc cgc cca ctg aac tcc 336Ala Glu Tyr Phe Ala Asn Lys Asp Ala Ala Pro Arg Pro Leu Asn Ser
100 105 110gtt ttg gat ctc ggc aaa atc gaa gcc acc gga ttt agc gca ccg acc 384Val Leu Asp Leu Gly Lys Ile Glu Ala Thr Gly Phe Ser Ala Pro Thr
115 120 125tgg cag acc cgc ctc aac gac tac ctc aag gaa ctc tca aag 426Trp Gln Thr Arg Leu Asn Asp Tyr Leu Lys Glu Leu Ser Lys
130 135 140tgaaaggcat catcctcgca ggt 449<210>446<211>142<212>PRT<213>谷氨酸棒杆菌<400>446Ala Pro Arg His Tyr Ile Val Arg Thr Ser Trp Val Ile Gly Asp Gly1 5 10 15Asn Asn Phe Val Arg Thr Met Lys Ser Leu Asp Glu Arg Gly Ile Ala
20 25 30Pro Ser Val Val Asp Asp Gln Ile Gly Arg Leu Ser Phe Thr Glu Asp
35 40 45Ile Ala Ala Gly Ile Ala His Leu Leu Glu Val Gly Ala Ala Tyr Gly
50 55 60Thr Tyr Asn Leu Thr Asn Thr Gly Glu Pro Ala Ser Trp Ala Asp Val65 70 75 80Ala Arg Ala Val Phe Ser Asp Pro Thr Lys Val Thr Gly Val Ser Thr
85 90 95Ala Glu Tyr Phe Ala Asn Lys Asp Ala Ala Pro Arg Pro Leu Asn Ser
100 105 110Val Leu Asp Leu Gly Lys Ile Glu Ala Thr Gly Phe Ser Ala Pro Thr
115 120 125Trp Gln Thr Arg Leu Asn Asp Tyr Leu Lys Glu Leu Ser Lys
130 135 140<210>447<211>1028<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1021)<223>FRXA02055<400>447aaggcctaga gcagaccatc gattggtacc gcgaaaacga ggcctggtgg cgccctgcca 60agaacaacgt cgaagctacc tacgctaagc agggacaata atg gaa tac ggt aaa 115
Met Glu Tyr Gly Lys
1 5caa ctc acc tcc cac acc acc gac atc gaa ggc cta ctg gtt ttc gat 163Gln Leu Thr Ser His Thr Thr Asp Ile Glu Gly Leu Leu Val Phe Asp
10 15 20ttc ccc gtc cac ggc gac aac cgc ggc tgg ttc aag gaa aat tgg cag 211Phe Pro Val His Gly Asp Asn Arg Gly Trp Phe Lys Glu Asn Trp Gln
25 30 35cgc acc aag atg acc aac ctg ggg ctg ccc gat ttt ggc ccc gtc caa 259Arg Thr Lys Met Thr Asn Leu Gly Leu Pro Asp Phe Gly Pro Val Gln
40 45 50aac aac atg agt ttc aac gcc acc gcc ggc acg act cgc ggc atg cac 307Asn Asn Met Ser Phe Asn Ala Thr Ala Gly Thr Thr Arg Gly Met His
55 60 65gct gag ccg tgg gat aaa ttt gtg tcc gtc gcg gtg ggt tcc gtt ttc 355Ala Glu Pro Trp Asp Lys Phe Val Ser Val Ala Val Gly Ser Val Phe70 75 80 85gga gct tgg gtg gat ctg cgc gcg ggc tcg agc acg tac ggt aac gtc 403Gly Ala Trp Val Asp Leu Arg Ala Gly Ser Ser Thr Tyr Gly Asn Val
90 95 100gta acg caa aaa att acc cct gac gtg gga gtt tac gtc ccg cgt ggt 451Val Thr Gln Lys Ile Thr Pro Asp Val Gly Val Tyr Val Pro Arg Gly
105 110 115gtg gca aac ggc ttc cag gcg ctc gag gac ggc acg ctg tac acc tac 499Val Ala Asn Gly Phe Gln Ala Leu Glu Asp Gly Thr Leu Tyr Thr Tyr
120 125 130ctc gtc aac gat cat tgg tcc ccc gac gcg cat tac gcc aac gtc aac 547Leu Val Asn Asp His Trp Ser Pro Asp Ala His Tyr Ala Asn Val Asn
135 140 145ctc aac atg atc gac tgg ccg ctg ccc atc acc gag atc tcc gaa aaa 595Leu Asn Met Ile Asp Trp Pro Leu Pro Ile Thr Glu Ile Ser Glu Lys150 155 160 165gat aaa aaa cat cca gcg ctt atc gac gcc acc ccc ctg ccc gcc cgc 643Asp Lys Lys His Pro Ala Leu Ile Asp Ala Thr Pro Leu Pro Ala Arg
170 175 180aag gtt ctc gtg gtc ggc gcc ggc gga caa ctg gga acc gcg cta cgc 691Lys Val Leu Val Val Gly Ala Gly Gly Gln Leu Gly Thr Ala Leu Arg
185 190 195gcg cag ttc cca gac gcg gaa ttt gtc acg cgc caa gaa ctc gat atc 739Ala Gln Phe Pro Asp Ala Glu Phe Val Thr Arg Gln Glu Leu Asp Ile
200 205 210acc tca gat ctc acc gag gct cgc gcg tgg aaa caa tac tcc acc atc 787Thr Ser Asp Leu Thr Glu Ala Arg Ala Trp Lys Gln Tyr Ser Thr Ile
215 220 225ata aac gcc gcc gcc tac act gcc gtt gac cag gca gaa cac gac cgc 835Ile Asn Ala Ala Ala Tyr Thr Ala Val Asp Gln Ala Glu His Asp Arg230 235 240 245gca gca gcg tgg gac att aac gca gcg gca gtg gct acc tcg cga cca 883Ala Ala Ala Trp Asp Ile Asn Ala Ala Ala Val Ala Thr Ser Arg Pro
250 255 260tcg cgc gcg aca aca acc tca ccc tcg tgc acg tgt cct cag att atg 931Ser Arg Ala Thr Thr Thr Ser Pro Ser Cys Thr Cys Pro Gln Ile Met
265 270 275tct tcg acg gtg cgg gcg aat cct acg atg aaa acg cac cgt ttt ccc 979Ser Ser Thr Val Arg Ala Asn Pro Thr Met Lys Thr His Arg Phe Pro
280 285 290cac tcg gcg tgt acg gcc aat cca aag cag ccg gcg aca tcg 1021His Ser Ala Cys Thr Ala Asn Pro Lys Gln Pro Ala Thr Ser
295 300 305taagcac 1028<210>448<211>307<212>PRT<213>谷氨酸棒杆菌<400>448Met Glu Tyr Gly Lys Gln Leu Thr Ser His Thr Thr Asp Ile Glu Gly1 5 10 15Leu Leu Val Phe Asp Phe Pro Val His Gly Asp Asn Arg Gly Trp Phe
20 25 30Lys Glu Asn Trp Gln Arg Thr Lys Met Thr Asn Leu Gly Leu Pro Asp
35 40 45Phe Gly Pro Val Gln Asn Asn Met Ser Phe Asn Ala Thr Ala Gly Thr
50 55 60Thr Arg Gly Met His Ala Glu Pro Trp Asp Lys Phe Val Ser Val Ala65 70 75 80Val Gly Ser Val Phe Gly Ala Trp Val Asp Leu Arg Ala Gly Ser Ser
85 90 95Thr Tyr Gly Asn Val Val Thr Gln Lys Ile Thr Pro Asp Val Gly Val
100 105 110Tyr Val Pro Arg Gly Val Ala Asn Gly Phe Gln Ala Leu Glu Asp Gly
115 120 125Thr Leu Tyr Thr Tyr Leu Val Asn Asp His Trp Ser Pro Asp Ala His
130 135 140Tyr Ala Asn Val Asn Leu Asn Met Ile Asp Trp Pro Leu Pro Ile Thr145 150 155 160Glu Ile Ser Glu Lys Asp Lys Lys His Pro Ala Leu Ile Asp Ala Thr
165 170 175Pro Leu Pro Ala Arg Lys Val Leu Val Val Gly Ala Gly Gly Gln Leu
180 185 190Gly Thr Ala Leu Arg Ala Gln Phe Pro Asp Ala Glu Phe Val Thr Arg
195 200 205Gln Glu Leu Asp Ile Thr Ser Asp Leu Thr Glu Ala Arg Ala Trp Lys
210 215 220Gln Tyr Ser Thr Ile Ile Asn Ala Ala Ala Tyr Thr Ala Val Asp Gln225 230 235 240Ala Glu His Asp Arg Ala Ala Ala Trp Asp Ile Asn Ala Ala Ala Val
245 250 255Ala Thr Ser Arg Pro Ser Arg Ala Thr Thr Thr Ser Pro Ser Cys Thr
260 265 270Cys Pro Gln Ile Met Ser Ser Thr Val Arg Ala Asn Pro Thr Met Lys
275 280 285Thr His Arg Phe Pro His Ser Ala Cys Thr Ala Asn Pro Lys Gln Pro
290 295 300Ala Thr Ser305<210>449<211>1056<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1033)<223>RXA00825<400>449cccgttcatg ctgggctttg gtgcggtgat ggcaactatt tgtctgatca ttgtgagttt 60tagtgcacgc cgattctgag aaacaactaa agtgagccac atg cgc aca gta gtt 115
Met Arg Thr Val Val
1 5acc ggc ggt gcc ggc ttc atc gga tcc cat ctc gtt gac ctt ttg atc 163Thr Gly Gly Ala Gly Phc Ile Gly Ser His Leu Val Asp Leu Leu Ile
10 15 20aag gaa ggc cac gag gtc gtt gtg atc gat aac ctc tcc cgc gga cgc 211Lys Glu Gly His Glu Val Val Val Ile Asp Asn Leu Ser Arg Gly Arg
25 30 35ctg gag aat crc tcc gat gcg gaa gcc acc gga aaa ctc acc ttt gtg 259Leu Glu Asn Leu Ser Asp Ala Glu Ala Thr Gly Lys Leu Thr Phe Val
40 45 50gaa gcc gat ctt ctc gac gtt gat ttc aac gag ttt cta gga acc cac 307Glu Ala Asp Leu Leu Asp Val Asp Phe Asn Glu Phe Leu Gly Thr His
55 60 65aag cct gag gtt att ttc cac ctg gca gcg caa atc gat gtg cgc cac 355Lys Pro Glu Val Ile Phe His Leu Ala Ala Gln Ile Asp Val Arg His70 75 80 85tct gtt gta gat cct ctt cac gac gcc gaa acc aac att ttg tcc acc 403Ser Val Val Asp Pro Leu His Asp Ala Glu Thr Asn Ile Leu Ser Thr
90 95 100atc cgc atc gct gac gct gcc cgc cag cac ggt gtt cgc aag gtt gtc 451Ile Arg Ile Ala Asp Ala Ala Arg Gln His Gly Val Arg Lys Val Val
105 110 115ttt acc tcc tca ggc ggt tcc att tac ggt gag cct tcg gaa ttc cca 499Phe Thr Ser Ser Gly Gly Ser Ile Tyr Gly Glu Pro Ser Glu Phe Pro
120 125 130gtt gat gaa acc gtg cca gtg gat cca cat tcc cct tat gcg gca tcc 547Val Asp Glu Thr Val Pro Val Asp Pro His Ser Pro Tyr Ala Ala Ser
135 140 145aag gtg tcc ggt gaa att tac ctg aac acc ttc cgc cac ctg tac ggc 595Lys Val Ser Gly Glu Ile Tyr Leu Asn Thr Phe Arg His Leu Tyr Gly150 155 160 165tta gac tgt tct cac atc gca ccg gca aat gtt tac ggc cca cgc caa 643Leu Asp Cys Ser His Ile Ala Pro Ala Asn Val Tyr Gly Pro Arg Gln
170 175 180gat cca cac ggt gaa gca gga gtt gtg gcc att ttc gcg ctg cga ctt 691Asp Pro His Gly Glu Ala Gly Val Val Ala Ile Phe Ala Leu Arg Leu
185 190 195ctg gga ggc ctg gac acc aag gta ttc ggc gac ggc gga aac acc cgc 739Leu Gly Gly Leu Asp Thr Lys Val Phe Gly Asp Gly Gly Asn Thr Arg
200 205 210gac tac gtc tac gtc ggt gac gta gtt cgt gct ttc tac ctg gct tct 787Asp Tyr Val Tyr Val Gly Asp Val Val Arg Ala Phe Tyr Leu Ala Ser
215 220 225ggg gaa atc ggt ggg gga gag cgc ttc aac att ggc acc tct gtg gaa 835Gly Glu Ile Gly Gly Gly Glu Arg Phe Asn Ile Gly Thr Ser Val Glu230 235 240 245acc tct gac cgc cag ctg cac acc ctc gtg gcc act gcg gca ggt tcc 883Thr Ser Asp Arg Gln Leu His Thr Leu Val Ala Thr Ala Ala Gly Ser
250 255 260aaa gat gat cct gaa tat gca cct gca cgt ctc ggc gat gtg cca cgc 931Lys Asp Asp Pro Glu Tyr Ala Pro Ala Arg Leu Gly Asp Val Pro Arg
265 270 275agt gca ctc agc ttc ggc aag gcc aaa gag gtg ctt ggt tgg gag cct 979Ser Ala Leu Ser Phe Gly Lys Ala Lys Glu Val Leu Gly Trp Glu Pro
280 285 290gag gtg aac atc gaa caa ggt gtg gcc aag act gtg gag tac ttc cgc 1027Glu Val Asn Ile Glu Gln Gly Val Ala Lys Thr Val Glu Tyr Phe Arg
295 300 305act cac taggggaaaa tccaccacaa atc 1056Thr His310<210>450<211>311<212>PRT<213>谷氨酸棒杆菌<400>450Met Arg Thr Val Val Thr Gly Gly Ala Gly Phe Ile Gly Ser His Leu1 5 10 15Val Asp Leu Leu Ile Lys Glu Gly His Glu Val Val Val Ile Asp Asn
20 25 30Leu Ser Arg Gly Arg Leu Glu Asn Leu Ser Asp Ala Glu Ala Thr Gly
35 40 45Lys Leu Thr Phe Val Glu Ala Asp Leu Leu Asp Val Asp Phe Asn Glu
50 55 60Phe Leu Gly Thr His Lys Pro Glu Val Ile Phe His Leu Ala Ala Gln65 70 75 80Ile Asp Val Arg His Ser Val Val Asp Pro Leu His Asp Ala Glu Thr
85 90 95Asn Ile Leu Ser Thr Ile Arg Ile Ala Asp Ala Ala Arg Gln His Gly
100 105 110Val Arg Lys Val Val Phe Thr Ser Ser Gly Gly Ser Ile Tyr Gly Glu
115 120 125Pro Ser Glu Phe Pro Val Asp Glu Thr Val Pro Val Asp Pro His Ser
130 135 140Pro Tyr Ala Ala Ser Lys Val Ser Gly Glu Ile Tyr Leu Asn Thr Phe145 150 155 160Arg His Leu Tyr Gly Leu Asp Cys Ser His Ile Ala Pro Ala Asn Val
165 170 175Tyr Gly Pro Arg Gln Asp Pro His Gly Glu Ala Gly Val Val Ala Ile
180 185 190Phe Ala Leu Arg Leu Leu Gly Gly Leu Asp Thr Lys Val Phe Gly Asp
195 200 205Gly Gly Asn Thr Arg Asp Tyr Val Tyr Val Gly Asp Val Val Arg Ala
210 215 220Phe Tyr Leu Ala Ser Gly Glu Ile Gly Gly Gly Glu Arg Phe Asn Ile225 230 235 240Gly Thr Ser Val Glu Thr Ser Asp Arg Gln Leu His Thr Leu Val Ala
245 250 255Thr Ala Ala Gly Ser Lys Asp Asp Pro Glu Tyr Ala Pro Ala Arg Leu
260 265 270Gly Asp Val Pro Arg Ser Ala Leu Ser Phe Gly Lys Ala Lys Glu Val
275 280 285Leu Gly Trp Glu Pro Glu Val Asn Ile Glu Gln Gly Val Ala Lys Thr
290 295 300Val Glu Tyr Phe Arg Thr His305 310<210>451<211>1140<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1117)<223>RXA02054<400>451cctaatgaac agccggagca ccctggtcgt ttgcagaata ggcgcatcga caacagctac 60taactctgcc agctcgcccg gacgaactaa ggtagacggc atg act tct ttg ctt 115
Met Thr Ser Leu Leu
1 5gtg acc gga ggt gcc gga ttt atc ggc gcc aac ttc gtc cgc caa acc 163Val Thr Gly Gly Ala Gly Phe Ile Gly Ala Asn Phe Val Arg Gln Thr
10 15 20gta gag cag cac cct gaa tac acc cac atc acg gtg ctg gat aaa ctc 211Val Glu Gln His Pro Glu Tyr Thr His Ile Thr Val Leu Asp Lys Leu
25 30 35acc tac gca gga aac gcc gac aat ctc aaa ggc ctc ccc gac agc aaa 259Thr Tyr Ala Gly Asn Ala Asp Asn Leu Lys Gly Leu Pro Asp Ser Lys
40 45 50gta acc ctc atc gaa ggc gat atc tgc gat gct gaa tta gtc gac tcc 307Val Thr Leu Ile Glu Gly Asp Ile Cys Asp Ala Glu Leu Val Asp Ser
55 60 65ctg gtc aaa gac cac gac atc aca gtc cac ttc gca gca gaa tcc cac 355Leu Val Lys Asp His Asp Ile Thr Val His Phe Ala Ala Glu Ser His70 75 80 85aac gac aac tcc ctc aac gac ccc tcc ccg ttt gtt cac act aac ctc 403Asn Asp Asn Ser Leu ASn Asp Pro Ser Pro Phe Val His Thr Asn Leu
90 95 100atc ggc acc ttt gtc ctg cta gaa gca gtc cgc aag cac aac aaa cgc 451Ile Gly Thr Phe Val Leu Leu Glu Ala Val Arg Lys His Asn Lys Arg
105 110 115ttc cac cac atc tcc acc gat gaa gtc ttc ggc gat cta gag ctg gat 499Phe His His Ile Ser Thr Asp Glu Val Phe Gly Asp Leu Glu Leu Asp
120 125 130gat cca aac cgc ttc act gaa acc acc gcc tac aag cca tcg tct cca 547Asp Pro Asn Arg Phe Thr Glu Thr Thr Ala Tyr Lys Pro Ser Ser Pro
135 140 145tat tct gca acc aag gca ggg tct gat cac ttg gta cac gca tgg atc 595Tyr Ser Ala Thr Lys Ala Gly Ser Asp His Leu Val His Ala Trp Ile150 155 160 165cgc tcc ttc gga atc cag gca acc atg tct aac tgc tcc aac aat tac 643Arg Ser Phe Gly Ile Gln Ala Thr Met Ser Asn Cys Ser Asn Asn Tyr
170 175 180ggt ccc tac cag cac att gaa aag ttc atc ccc cgc cag atc acc aat 691Gly Pro Tyr Gln His Ile Glu Lys Phe Ile Pro Arg Gln Ile Thr Asn
185 190 195att ctg gcc ggc ctg aca cca aaa ctt tat gga acc ggc gag cag gtc 739Ile Leu Ala Gly Leu Thr Pro Lys Leu Tyr Gly Thr Gly Glu Gln Val
200 205 210cgc gac tgg atc cac gtc gat gat cac aat gac gcc gtc cac ctg atc 787Arg Asp Trp Ile His Val Asp Asp His Asn Asp Ala Val His Leu Ile
215 220 225ctg agt aag ggc aag atc ggc gaa acc tac atc atc ggc gcc gac aac 835Leu Ser Lys Gly Lys Ile Gly Glu Thr Tyr Ile Ile Gly Ala Asp Asn230 235 240 245gat cat gtg aat aac aag cag gtc atc gag ctt att tgt gaa ctc atg 883Asp His Val Asn Asn Lys Gln Val Ile Glu Leu Ile Cys Glu Leu Met
250 255 260ggc ctc gac aaa aac gca tac gag cac gtc gca gac cgc ccc ggc cac 931Gly Leu Asp Lys Asn Ala Tyr Glu His Val Ala Asp Arg Pro Gly His
265 270 275gat atg cgt tac gcc atg gat tcc acc aag ctg cgc acc gag ctc ggc 979Asp Met Arg Tyr Ala Met Asp Ser Thr Lys Leu Arg Thr Glu Leu Gly
280 285 290tgg gca cct aaa tac acc gac gtt gat tcc ggc atg cgc aaa ggc cta 1027Trp Ala Pro Lys Tyr Thr Asp Val Asp Ser Gly Met Arg Lys Gly Leu
295 300 305gag cag acc atc gat tgg tac cgc gaa aac gag gcc tgg tgg cgc cct 1075Glu Gln Thr Ile Asp Trp Tyr Arg Glu Asn Glu Ala Trp Trp Arg Pro310 315 320 325gcc aag aac aac gtc gaa gct acc tac gct aag cag gga caa 1117Ala Lys Asn Asn Val Glu Ala Thr Tyr Ala Lys Gln Gly Gln
330 335taatggaata cggtaaacaa ctc 1140<210>452<211>339<212>PRT<213>谷氨酸棒杆菌<400>452Met Thr Ser Leu Leu Val Thr Gly Gly Ala Gly Phe Ile Gly Ala Asn1 5 10 15Phe Val Arg Gln Thr Val Glu Gln His Pro Glu Tyr Thr His Ile Thr
20 25 30Val Leu Asp Lys Leu Thr Tyr Ala Gly Asn Ala Asp Asn Leu Lys Gly
35 40 45Leu Pro Asp Ser Lys Val Thr Leu Ile Glu Gly Asp Ile Cys Asp Ala
50 55 60Glu Leu Val Asp Ser Leu Val Lys Asp His Asp Ile Thr Val His Phe65 70 75 80Ala Ala Glu Ser His Asn Asp Asn Ser Leu Asn Asp Pro Ser Pro Phe
85 90 95Val His Thr Asn Leu Ile Gly Thr Phe Val Leu Leu Glu Ala Val Arg
100 105 110Lys His Asn Lys Arg Phe His His Ile Ser Thr Asp Glu Val Phe Gly
115 120 125Asp Leu Glu Leu Asp Asp Pro Asn Arg Phe Thr Glu Thr Thr Ala Tyr
130 135 140Lys Pro Ser Ser Pro Tyr Ser Ala Thr Lys Ala Gly Ser Asp His Leu145 150 155 160Val His Ala Trp Ile Arg Ser Phe Gly Ile Gln Ala Thr Met Ser Asn
165 170 175Cys Ser Asn Asn Tyr Gly Pro Tyr Gln His Ile Glu Lys Phe Ile Pro
180 185 190Arg Gln Ile Thr Asn Ile Leu Ala Gly Leu Thr Pro Lys Leu Tyr Gly
195 200 205Thr Gly Glu Gln Val Arg Asp Trp Ile His Val Asp Asp His Asn Asp
210 215 220Ala Val His Leu Ile Leu Ser Lys Gly Lys Ile Gly Glu Thr Tyr Ile225 230 235 240Ile Gly Ala Asp Asn Asp His Val Asn Asn Lys Gln Val Ile Glu Leu
245 250 255Ile Cys Glu Leu Met Gly Leu Asp Lys Asn Ala Tyr Glu His Val Ala
260 265 270Asp Arg Pro Gly His Asp Met Arg Tyr Ala Met Asp Ser Thr Lys Leu
275 280 285Arg Thr Glu Leu Gly Trp Ala Pro Lys Tyr Thr Asp Val Asp Ser Gly
290 295 300Met Arg Lys Gly Leu Glu Gln Thr Ile Asp Trp Tyr Arg Glu Asn Glu305 310 315 320Ala Trp Trp Arg Pro Ala Lys Asn Asn Val Glu Ala Thr Tyr Ala Lys
325 330 335Gln Gly Gln<210>453<211>909<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(886)<223>RXN00427<400>453gcggtcattg tgacccacaa tcgtgtggaa ttgctgcggc attccctcga ggttgttgcc 60aatcaaacct atccggtgaa acacattgtg gtggtggata atg ggg cgg atc cta 115
Met Gly Arg Ile Leu
1 5gta ttc tat agt gtc act tta gag gtg gcg gga gac cgt ccc gtt tac 163Val Phe Tyr Ser Val Thr Leu Glu Val Ala Gly Asp Arg Pro Val Tyr
10 15 20acg cct tcg cgc acc aat ttg ggc gac ggt ggc ggt ttt gct ttt ggt 211Thr Pro Ser Arg Thr Asn Leu Gly Asp Gly Gly Gly Phe Ala Phe Gly
25 30 35ttt ttg acg gcg ttg gcg ctg ggg gcg aac gcg gtg tgg tgc aca gac 259Phe Leu Thr Ala Leu Ala Leu Gly Ala Asn Ala Val Trp Cys Thr Asp
40 45 50gat gac ggc cgg ccg gag ggg cca ggg gtg ttg aag acg ctt atc gac 307Asp Asp Gly Arg Pro Glu Gly Pro Gly Val Leu Lys Thr Leu Ile Asp
55 60 65gcc gct tct cgg cat aat ctg gag gag gtt tct ccg gtg gta tgc aat 355Ala Ala Ser Arg His Asn Leu Glu Glu Val Ser Pro Val Val Cys Asn70 75 80 85gct gat gat ccg gag cgg ttg gca ttt ccg ctg cgt cgg ggc ttg gag 403Ala Asp Asp Pro Glu Arg Leu Ala Phe Pro Leu Arg Arg Gly Leu Glu
90 95 100tgg cgt cgg atg cgc agt gag ttg att gat cca gcc aac ccg gag gat 451Trp Arg Arg Met Arg Ser Glu Leu Ile Asp Pro Ala Asn Pro Glu Asp
105 110 115gat ttg ctg ccg ggc atc gcc tcc ttg ttc aat ggt gcc ctg atc agc 499Asp Leu Leu Pro Gly Ile Ala Ser Leu Phe Asn Gly Ala Leu Ile Ser
120 125 130gct tat gca atg gag cgc att ggc gtg ccg gac tat cga ctg ttt att 547Ala Tyr Ala Met Glu Arg Ile Gly Val Pro Asp Tyr Arg Leu Phe Ile
135 140 145cgc ggc gat gag gtg gag tat cac cgc cgt ttg gtg cgt tcc ggt ttg 595Arg Gly Asp Glu Val Glu Tyr His Arg Arg Leu Val Arg Ser Gly Leu150 155 160 165ccg ttt ggt acg tgt ttg acc acg gcg tat ttg cac ccg gat ggt tct 643Pro Phe Gly Thr Cys Leu Thr Thr Ala Tyr Leu His Pro Asp Gly Ser
170 175 180gat gag ttc aag ccg att ctg ggt ggg cgg atg cat acg cag tat ccg 691Asp Glu Phe Lys Pro Ile Leu Gly Gly Arg Met His Thr Gln Tyr Pro
185 190 195gat aat gat ttc aag agg ttt ttc acc tac cgc aac cgt ggc tac ctg 739Asp Asn Asp Phe Lys Arg Phe Phe Thr Tyr Arg Asn Arg Gly Tyr Leu
200 205 210atg agc cag ccg gga atg cgc aag ctt ctc cct cag gaa tat gcg cgc 787Met Ser Gln Pro Gly Met Arg Lys Leu Leu Pro Gln Glu Tyr Ala Arg
215 220 225ttt gcg tgg ttc ttc ctg gtt cag aaa cgg gat gtg aag gga ttc cgg 835Phe Ala Trp Phe Phe Leu Val Gln Lys Arg Asp Val Lys Gly Phe Arg230 235 240 245gag tgg ctg cgc ctg cac aaa ctg ggc cgc gac gag aaa ttc aat agg 883Glu Trp Leu Arg Leu His Lys Leu Gly Arg Asp Glu Lys Phe Asn Arg
250 255 260ccc tagatcagtt ttagtagttc ctc 909Pro<210>454<211>262<212>PRT<213>谷氨酸棒杆菌<400>454Met Gly Arg Ile Leu Val Phe Tyr Ser Val Thr Leu Glu Val Ala Gly1 5 10 15Asp Arg Pro Val Tyr Thr Pro Ser Arg Thr Asn Leu Gly Asp Gly Gly
20 25 30Gly Phe Ala Phe Gly Phe Leu Thr Ala Leu Ala Leu Gly Ala Asn Ala
35 40 45Val Trp Cys Thr Asp Asp Asp Gly Arg Pro Glu Gly Pro Gly Val Leu
50 55 60Lys Thr Leu Ile Asp Ala Ala Ser Arg His Asn Leu Glu Glu Val Ser65 70 75 80Pro Val Val Cys Asn Ala Asp Asp Pro Glu Arg Leu Ala Phe Pro Leu
85 90 95Arg Arg Gly Leu Glu Trp Arg Arg Met Arg Ser Glu Leu Ile Asp Pro
100 105 110Ala Asn Pro Glu Asp Asp Leu Leu Pro Gly Ile Ala Ser Leu Phe Asn
115 120 125Gly Ala Leu Ile Ser Ala Tyr Ala Met Glu Arg Ile Gly Val Pro Asp
130 135 140Tyr Arg Leu Phe Ile Arg Gly Asp Glu Val Glu Tyr His Arg Arg Leu145 150 155 160Val Arg Ser Gly Leu Pro Phe Gly Thr Cys Leu Thr Thr Ala Tyr Leu
165 170 175His Pro Asp Gly Ser Asp Glu Phe Lys Pro Ile Leu Gly Gly Arg Met
180 185 190His Thr Gln Tyr Pro Asp Asn Asp Phe Lys Arg Phe Phe Thr Tyr Arg
195 200 205Asn Arg Gly Tyr Leu Met Ser Gln Pro Gly Met Arg Lys Leu Leu Pro
210 215 220Gln Glu Tyr Ala Arg Phe Ala Trp Phe Phe Leu Val Gln Lys Arg Asp225 230 235 240Val Lys Gly Phe Arg Glu Trp Leu Arg Leu His Lys Leu Gly Arg Asp
245 250 255Glu Lys Phe Asn Arg Pro
260<210>455<211>555<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(532)<223>FRXA00427<400>455tgctgatgat ccggagcggt tggcatttcc gctgcgtcgg ggcttggagt ggcgtcggat 60gcgcagtgag ttgattgatc cagccaaccc ggaggatgat ttg ctg ccg ggc atc 115
Leu Leu Pro Gly Ile
1 5gcc tcc ttg ttc aat ggt gcc ctg atc agc gct tat gca atg gag cgc 163Ala Ser Leu Phe Asn Gly Ala Leu Ile Ser Ala Tyr Ala Met Glu Arg
10 15 20att ggc gtg ccg gac tat cga ctg ttt att cgc ggc gat gag gtg gag 211Ile Gly Val Pro Asp Tyr Arg Leu Phe Ile Arg Gly Asp Glu Val Glu
25 30 35tat cac cgc cgt ttg gtg cgt tcc ggt ttg ccg ttt ggt acg tgt ttg 259Tyr His Arg Arg Leu Val Arg Ser Gly Leu Pro Phe Gly Thr Cys Leu
40 45 50acc acg gcg tat ttg cac ccg gat ggt tct gat gag ttc aag ccg att 307Thr Thr Ala Tyr Leu His Pro Asp Gly Ser Asp Glu Phe Lys Pro Ile
55 60 65ctg ggt ggg cgg atg cat acg cag tat ccg gat aat gat ttc aag agg 355Leu Gly Gly Arg Met His Thr Gln Tyr Pro Asp Asn Asp Phe Lys Arg70 75 80 85ttt ttc acc tac cgc aac cgt ggc tac ctg atg agc cag ccg gga atg 403Phe Phe Thr Tyr Arg Asn Arg Gly Tyr Leu Met Ser Gln Pro Gly Met
90 95 100cgc aag ctt ctc cct cag gaa tat gcg cgc ttt gcg tgg ttc ttc ctg 451Arg Lys Leu Leu Pro Gln Glu Tyr Ala Arg Phe Ala Trp Phe Phe Leu
105 110 115gtt cag aaa cgg gat gtg aag gga ttc cgg gag tgg ctg cgc ctg cac 499Val Gln Lys Arg Asp Val Lys Gly Phe Arg Glu Trp Leu Arg Leu His
120 125 130aaa ctg ggc cgc gac gag aaa ttc aat agg ccc tagatcagtt ttagtagttc 552Lys Leu Gly Arg Asp Glu Lys Phe Asn Arg Pro
135 140ctc 555<210>456<211>144<212>PRT<213>谷氨酸棒杆菌<400>456Leu Leu Pro Gly Ile Ala Ser Leu Phe Asn Gly Ala Leu Ile Ser Ala1 5 10 15Tyr Ala Met Glu Arg Ile Gly Val Pro Asp Tyr Arg Leu Phe Ile Arg
20 25 30Gly Asp Glu Val Glu Tyr His Arg Arg Leu Val Arg Ser Gly Leu Pro
35 40 45Phe Gly Thr Cys Leu Thr Thr Ala Tyr Leu His Pro Asp Gly Ser Asp
50 55 60Glu Phe Lys Pro Ile Leu Gly Gly Arg Met His Thr Gln Tyr Pro Asp65 70 75 80Asn Asp Phe Lys Arg Phe Phe Thr Tyr Arg Asn Arg Gly Tyr Leu Met
85 90 95Ser Gln Pro Gly Met Arg Lys Leu Leu Pro Gln Glu Tyr Ala Arg Phe
100 105 110Ala Trp Phe Phe Leu Val Gln Lys Arg Asp Val Lys Gly Phe Arg Glu
115 120 125Trp Leu Arg Leu His Lys Leu Gly Arg Asp Glu Lys Phe Asn Arg Pro
130 135 140<210>457<211>507<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(484)<223>RXA00327<400>457aacgattttc gccgtcgcaa ccgtgatcac cggtcgcttt ccggcatcgt gtcgtcgcgg 60ctagggggcc aggcgcggga tgattgggtt tgtcacgccg atg att cag gcg gcg 115
Met Ile Gln Ala Ala
1 5ttg tgg atc gtg ctc ttt tta ttc gct gat cgc ctg tcc aat ccg ctg 163Leu Trp Ile Val Leu Phe Leu Phe Ala Asp Arg Leu Ser Asn Pro Leu
10 15 20gtt ttt gtc agc gcc atc atg ttc gcg att tcc ttt agc tcc ccc gtg 211Val Phe Val Ser Ala Ile Met Phe Ala Ile Ser Phe Ser Ser Pro Val
25 30 35gcg aac ttc ggt ttc gat acg atc tgc gaa aaa ctc gac cgc cgc gtc 259Ala Asn Phe Gly Phe Asp Thr Ile Cys Glu Lys Leu Asp Arg Arg Val
40 45 50atg gtc gcc ggc acc ggc atg gcc aac atg agc gcc tac att tgc gcg 307Met Val Ala Gly Thr Gly Met Ala Asn Met Ser Ala Tyr Ile Cys Ala
55 60 65atg ctg gcc aca caa atc atc gga ttt tta ctc gac tgg aac gcc gac 355Met Leu Ala Thr Gln Ile Ile Gly Phe Leu Leu Asp Trp Asn Ala Asp70 75 80 85ggc cac gcc tac acc tgg tcg aat ttc cag gtg gcg tgg ctt ggt ctg 403Gly His Ala Tyr Thr Trp Ser Asn Phe Gln Val Ala Trp Leu Gly Leu
90 95 100ggc gcg gtg tgg ctg gca ggc atg atc ggg ctt gca gtc tgc ctc ctg 451Gly Ala Val Trp Leu Ala Gly Met Ile Gly Leu Ala Val Cys Leu Leu
105 110 115ctg cag cgt cga aaa aat att gct ttt cga cgc taaaacccga ccgtaaccgc 504Leu Gln Arg Arg Lys Asn Ile Ala Phe Arg Arg
120 125tag 507<210>458<211>128<212>PRT<213>谷氨酸棒杆菌<400>458Met Ile Gln Ala Ala Leu Trp Ile Val Leu Phe Leu Phe Ala Asp Arg1 5 10 15Leu Ser Asn Pro Leu Val Phe Val Ser Ala Ile Met Phe Ala Ile Ser
20 25 30Phe Ser Ser Pro Val Ala Asn Phe Gly Phe Asp Thr Ile Cys Glu Lys
35 40 45Leu Asp Arg Arg Val Met Val Ala Gly Thr Gly Met Ala Asn Met Ser
50 55 60Ala Tyr Ile Cys Ala Met Leu Ala Thr Gln Ile Ile Gly Phe Leu Leu65 70 75 80Asp Trp Asn Ala Asp Gly His Ala Tyr Thr Trp Ser Asn Phe Gln Val
85 90 95Ala Trp Leu Gly Leu Gly Ala Val Trp Leu Ala Gly Met Ile Gly Leu
100 105 110Ala Val Cys Leu Leu Leu Gln Arg Arg Lys Asn Ile Ala Phe Arg Arg
115 120 125<210>459<211>615<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(592)<223>RXA00328<400>459gtcacgggat ttgttgtagc ccgactcaag ggtggaccgg gccgcagatt actcgcccag 60attaggtcta ccaaggactt ttaaagatcg ggtaaaacct gtg ggc gtt gaa agg 115
Val Gly Val Glu Arg
1 5ggc aag gtc tct gcc aag gca cta gtt gtc tgg ttg acg gca atg tgc 163Gly Lys Val Ser Ala Lys Ala Leu Val Val Trp Leu Thr Ala Met Cys
10 15 20gtg tac atc gtg gcc att gct ggt cga aca tca ttt ggt gtc gcc gga 211Val Tyr Ile Val Ala Ile Ala Gly Arg Thr Ser Phe Gly Val Ala Gly
25 30 35gtg cat gcg atc gat cgc ttt gat atc gac gcc tcc cgg ttg gcc gtg 259Val His Ala Ile Asp Arg Phe Asp Ile Asp Ala Ser Arg Leu Ala Val
40 45 50ttc act tct gtt cag gtt gga gtc tat gtt ctc gcg cag att ccc atg 307Phe Thr Ser Val Gln Val Gly Val Tyr Val Leu Ala Gln Ile Pro Met
55 60 65ggc atg ctg gtg gac agg ttc gac gcc cgg aaa ctc ttg ctg gct ggc 355Gly Met Leu Val Asp Arg Phe Asp Ala Arg Lys Leu Leu Leu Ala Gly70 75 80 85gct tta att ttg gca gct ggc cag ctc att ttg ggt ttc act gat tct 403Ala Leu Ile Leu Ala Ala Gly Gln Leu Ile Leu Gly Phe Thr Asp Ser
90 95 100tat atg atc gcc att ttt gcc cga gtg ctc atc agt gtt ggc gat tct 451Tyr Met Ile Ala Ile Phe Ala Arg Val Leu Ile Ser Val Gly Asp Ser
105 110 115tct gca ttt ttg tcc gtg atg cga ctg ttg ccc aac tgg ttc ccg atg 499Ser Ala Phe Leu Ser Val Met Arg Leu Leu Pro Asn Trp Phe Pro Met
120 125 130tct tgg aca cct gtg ttg cag cag ctc acg ggc gct ttt ggc ttt gtg 547Ser Trp Thr Pro Val Leu Gln Gln Leu Thr Gly Ala Phe Gly Phe Val
135 140 145ggg cag ttt tct ccg cgg tgc cgt ttt tgc aca tac tca aca cct 592Gly Gln Phe Ser Pro Arg Cys Arg Phe Cys Thr Tyr Ser Thr Pro150 155 160tagggtggac aattcctttc gca 615<210>460<211>164<212>PRT<213>谷氨酸棒杆菌<400>460Val Gly Val Glu Arg Gly Lys Val Ser Ala Lys Ala Leu Val Val Trp1 5 10 15Leu Thr Ala Met Cys Val Tyr Ile Val Ala Ile Ala Gly Arg Thr Ser
20 25 30Phe Gly Val Ala Gly Val His Ala Ile Asp Arg Phe Asp Ile Asp Ala
35 40 45Ser Arg Leu Ala Val Phe Thr Ser Val Gln Val Gly Val Tyr Val Leu
50 55 60Ala Gln Ile Pro Met Gly Met Leu Val Asp Arg Phe Asp Ala Arg Lys65 70 75 80Leu Leu Leu Ala Gly Ala Leu Ile Leu Ala Ala Gly Gln Leu Ile Leu
85 90 95Gly Phe Thr Asp Ser Tyr Met Ile Ala Ile Phe Ala Arg Val Leu Ile
100 105 110Ser Val Gly Asp Ser Ser Ala Phe Leu Ser Val Met Arg Leu Leu Pro
115 120 125Asn Trp Phe Pro Met Ser Trp Thr Pro Val Leu Gln Gln Leu Thr Gly
130 135 140Ala Phe Gly Phe Val Gly Gln Phe Ser Pro Arg Cys Arg Phe Cys Thr145 150 155 160Tyr Ser Thr Pro<210>461<211>1347<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1324)<223>RXA00329<400>461tgtgaagaac aatgatcaac ggccttcgcc aagaccgcat cccagagaga aagtaactac 60ccgcgccctc atagtttggg cagcagcctg tcttgtgtat atg gca gcc att acc 115
Met Ala Ala Ile Thr
1 5agc cgc acc agc ttt ggt gtg gct gga gtg gag gcc att gat cgt ttt 163Ser Arg Thr Ser Phe Gly Val Ala Gly Val Glu Ala Ile Asp Arg Phe
10 15 20cag gta gac gcc aca cgc att gca gta ttc act tct gtt cag gtt ggt 211Gln Val Asp Ala Thr Arg Ile Ala Val Phe Thr Ser Val Gln Val Gly
25 30 35gtg tac gcg ttt gct cag att ccg atg ggc att ttg atc gat aag ttt 259Val Tyr Ala Phe Ala Gln Ile Pro Met Gly Ile Leu Ile Asp Lys Phe
40 45 50ggt cct cgg aag ctc ctt gcc att ggt gct ttg gtg atg ggt atc ggc 307Gly Pro Arg Lys Leu Leu Ala Ile Gly Ala Leu Val Met Gly Ile Gly
55 60 65cag ctc att ttg ggc ttt acg gat agc tat tcc atc gcg att att gcc 355Gln Leu Ile Leu Gly Phe Thr Asp Ser Tyr Ser Ile Ala Ile Ile Ala70 75 80 85cgc gtg ttt atc ggc gcc ggc gat gca tcg atc ttc ctt tcg gtt atg 403Arg Val Phe Ile Gly Ala Gly Asp Ala Ser Ile Phe Leu Ser Val Met
90 95 100cgc atc ctg ccg ttt tgg ttc cca ctg aag cac acc cct att ttc acg 451Arg Ile Leu Pro Phe Trp Phe Pro Leu Lys His Thr Pro Ile Phe Thr
105 110 115cag tta act acc tgc ctt ggc cag ttg ggc cag ttc ttt tct gcg gtg 499Gln Leu Thr Thr Cys Leu Gly Gln Leu Gly Gln Phe Phe Ser Ala Val
120 125 130cct ttc atg gcg ttg ttg ggt gcg cag ggt tgg cct gtg gcg ttt gtc 547Pro Phe Met Ala Leu Leu Gly Ala Gln Gly Trp Pro Val Ala Phe Val
135 140 145agc ctt ggt tcc gtg gtg gca ctc att gcg atc gca gcg ctg gtg gcc 595Ser Leu Gly Ser Val Val Ala Leu Ile Ala Ile Ala Ala Leu Val Ala150 155 160 165gtt cgg gat act cca gat cct cag cca aaa cct gtc gaa tca gct cag 643Val Arg Asp Thr Pro Asp Pro Gln Pro Lys Pro Val Glu Ser Ala Gln
170 175 180gaa gca gat aaa cca agc ctg cgc gcg agt ttg aag ttg att gtc cgc 691Glu Ala Asp Lys Pro Ser Leu Arg Ala Ser Leu Lys Leu Ile Val Arg
185 190 195aat ccg att acg tgg cag ggt ttc ttc att cac tac gta ttg atg gtg 739Asn Pro Ile Thr Trp Gln Gly Phe Phe Ile His Tyr Val Leu Met Val
200 205 210tgg cag acc gtg ttc tcc atg atg tgg ggc gtc ccg ctg atg act ttg 787Trp Gln Thr Val Phe Ser Met Met Trp Gly Val Pro Leu Met Thr Leu
215 220 225ggc atg gga ctg tct gca acg acg gct ggt ttg gtg ttg agc atc aac 835Gly Met Gly Leu Ser Ala Thr Thr Ala Gly Leu Val Leu Ser Ile Asn230 235 240 245acg ctg tgc atg gtg gta tcg gcg cca atc atc gga ata att tcc gca 883Thr Leu Cys Met Val Val Ser Ala Pro Ile Ile Gly Ile Ile Ser Ala
250 255 260cgc ctt ggg tat cgc cgt gac gtg gtc gcc att gcg ctg tcg ttt gtt 931Arg Leu Gly Tyr Arg Arg Asp Val Val Ala Ile Ala Leu Ser Phe Val
265 270 275caa tcc gca gta tgg ctg gtg ttc ttg gcc tcc gat gca cct cgt ggt 979Gln Ser Ala Val Trp Leu Val Phe Leu Ala Ser Asp Ala Pro Arg Gly
280 285 290ttg atg gct atc atc ttg gtc aac atc gtc atg ggt ctg act act gcg 1027Leu Met Ala Ile Ile Leu Val Asn Ile Val Met Gly Leu Thr Thr Ala
295 300 305gct tct ggt tat ggc ttt gac acc att cgt gag cgc cta gat cgc aag 1075Ala Ser Gly Tyr Gly Phe Asp Thr Ile Arg Glu Arg Leu Asp Arg Lys310 315 320 325att ttg gct gcg ggc acg gga ctg gca aac atg ggt gga ttc ttg tca 1123Ile Leu Ala Ala Gly Thr Gly Leu Ala Asn Met Gly Gly Phe Leu Ser
330 335 340tcg atg gtt gca gcg cag gtt atg ggg ttc ctt ctt gat cac agc gcg 1171Ser Met Val Ala Ala Gln Val Met Gly Phe Leu Leu Asp His Ser Ala
345 350 355cat ggt agc acc tat act tgg gtg gac ttc cgt ttt ggt ttc ctt gcg 1219His Gly Ser Thr Tyr Thr Trp Val Asp Phe Arg Phe Gly Phe Leu Ala
360 365 370att ctt gtc aca tgg gcc gtc gga gtc acg gga ttt gtt gta gcc cga 1267Ile Leu Val Thr Trp Ala Val Gly Val Thr Gly Phe Val Val Ala Arg
375 380 385ctc aag ggt gga ccg ggc cgc aga tta ctc gcc cag att agg tct acc 1315Leu Lys Gly Gly Pro Gly Arg Arg Leu Leu Ala Gln Ile Arg Ser Thr390 395 400 405aag gac ttt taaagatcgg gtaaaacctg tgg 1347Lys Asp Phe<210>462<211>408<212>PRT<213>谷氨酸棒杆菌<400>462Met Ala Ala Ile Thr Ser Arg Thr Ser Phe Gly Val Ala Gly Val Glu1 5 10 15Ala Ile Asp Arg Phe Gln Val Asp Ala Thr Arg Ile Ala Val Phe Thr
20 25 30Ser Val Gln Val Gly Val Tyr Ala Phe Ala Gln Ile Pro Met Gly Ile
35 40 45Leu Ile Asp Lys Phe Gly Pro Arg Lys Leu Leu Ala Ile Gly Ala Leu
50 55 60Val Met Gly Ile Gly Gln Leu Ile Leu Gly Phe Thr Asp Ser Tyr Ser65 70 75 80Ile Ala Ile Ile Ala Arg Val Phe Ile Gly Ala Gly Asp Ala Ser Ile
85 90 95Phe Leu Ser Val Met Arg Ile Leu Pro Phe Trp Phe Pro Leu Lys His
100 105 110Thr Pro Ile Phe Thr Gln Leu Thr Thr Cys Leu Gly Gln Leu Gly Gln
115 120 125Phe Phe Ser Ala Val Pro Phe Met Ala Leu Leu Gly Ala Gln Gly Trp
130 135 140Pro Val Ala Phe Val Ser Leu Gly Ser Val Val Ala Leu Ile Ala Ile145 150 155 160Ala Ala Leu Val Ala Val Arg Asp Thr Pro Asp Pro Gln Pro Lys Pro
165 170 175Val Glu Ser Ala Gln Glu Ala Asp Lys Pro Ser Leu Arg Ala Ser Leu
180 185 190Lys Leu Ile Val Arg Asn Pro Ile Thr Trp Gln Gly Phe Phe Ile His
195 200 205Tyr Val Leu Met Val Trp Gln Thr Val Phe Ser Met Met Trp Gly Val
210 215 220Pro Leu Met Thr Leu Gly Met Gly Leu Ser Ala Thr Thr Ala Gly Leu225 230 235 240Val Leu Ser Ile Asn Thr Leu Cys Met Val Val Ser Ala Pro Ile Ile
245 250 255Gly Ile Ile Ser Ala Arg Leu Gly Tyr Arg Arg Asp Val Val Ala Ile
260 265 270Ala Leu Ser Phe Val Gln Ser Ala Val Trp Leu Val Phe Leu Ala Ser
275 280 285Asp Ala Pro Arg Gly Leu Met Ala Ile Ile Leu Val Asn Ile Val Met
290 295 300Gly Leu Thr Thr Ala Ala Ser Gly Tyr Gly Phe Asp Thr Ile Arg Glu305 310 315 320Arg Leu Asp Arg Lys Ile Leu Ala Ala Gly Thr Gly Leu Ala Asn Met
325 330 335Gly Gly Phe Leu Ser Ser Met Val Ala Ala Gln Val Met Gly Phe Leu
340 345 350Leu Asp His Ser Ala His Gly Ser Thr Tyr Thr Trp Val Asp Phe Arg
355 360 365Phe Gly Phe Leu Ala Ile Leu Val Thr Trp Ala Val Gly Val Thr Gly
370 375 380Phe Val Val Ala Arg Leu Lys Gly Gly Pro Gly Arg Arg Leu Leu Ala385 390 395 400Gln Ile Arg Ser Thr Lys Asp Phe
405<210>463<211>2265<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2242)<223>RXN01554<400>463tggcttaaac cttatttgta gttgtcaata aatatgaaat tccttagcaa cttgtttaaa 60tagacgtata aacaagtttg aaaaaggaag gttatccatc ttg aaa aag cat gtg 115
Leu Lys Lys His Val
1 5acc tca gcc gtt acc gcc gtg gtg acg gct ttt tca acg gct gcg ctt 163Thr Ser Ala Val Thr Ala Val Val Thr Ala Phe Ser Thr Ala Ala Leu
10 15 20ggt tta agt att gcc gtt tct cct gct gtt gcc caa gtg gct aat cca 211Gly Leu Ser Ile Ala Val Ser Pro Ala Val Ala Gln Val Ala Asn Pro
25 30 35gct cca gat ctt tct gcg ccg tat aca tgg gtg gaa gag ttt gat tcc 259Ala Pro Asp Leu Ser Ala Pro Tyr Thr Trp Val Glu Glu Phe Asp Ser
40 45 50gag gat gct ctc aaa ggg tgg aac att ttc cgc cag cca gat tat ggc 307Glu Asp Ala Leu Lys Gly Trp Asn Ile Phe Arg Gln Pro Asp Tyr Gly
55 60 65agc gac aaa gtt ctc tat acc gaa gat gct tta agt atc gaa gat ggc 355Ser Asp Lys Val Leu Tyr Thr Glu Asp Ala Leu Ser Ile Glu Asp Gly70 75 80 85aag ctc acc atc acc act cag cgc cac tgc gtt gac gaa gac ttc gcg 403Lys Leu Thr Ile Thr Thr Gln Arg His Cys Val Asp Glu Asp Phe Ala
90 95 100atc agt gat cct gtc aac cgc gga aag ctc aat gac agc acc gcg caa 451Ile Ser Asp Pro Val Asn Arg Gly Lys Leu Asn Asp Ser Thr Ala Gln
105 110 115gtt gaa cct tgt gct cca ggt cag ttt gaa aag ttc acc agt gcg cgc 499Val Glu Pro Cys Ala Pro Gly Gln Phe Glu Lys Phe Thr Ser Ala Arg
120 125 130atc gtc act ccg aaa att gct cgt gga gag ttc gac ctt tct gtc act 547Ile Val Thr Pro Lys Ile Ala Arg Gly Glu Phe Asp Leu Ser Val Thr
135 140 145gca act ctt aac acc ggt ggc gtc gaa ggt gtc cga agt gcc att tgg 595Ala Thr Leu Asn Thr Gly Gly Val Glu Gly Val Arg Ser Ala Ile Trp150 155 160 165atg caa aac ggt gaa cag gcg tgt tcc tca gca acc aac aat ggc ctc 643Met Gln Asn Gly Glu Gln Ala Cys Ser Ser Ala Thr Asn Asn Gly Leu
170 175 180tac gga gaa cta gac ctg gta gag cac ttt tct tac gat ctt cgc tcg 691Tyr Gly Glu Leu Asp Leu Val Glu His Phe Ser Tyr Asp Leu Arg Ser
185 190 195cca tgg tct cca tca aac acc cac ttg ggt tgt gat cct gaa agt gtc 739Pro Trp Ser Pro Ser Asn Thr His Leu Gly Cys Asp Pro Glu Ser Val
200 205 210aac ggc acc aac cgt gca cct cgt gaa ctt aaa cta gat gag tca ctc 787Asn Gly Thr Asn Arg Ala Pro Arg Glu Leu Lys Leu Asp Glu Ser Leu
215 220 225gat ggc gtg gag cac acc tgg act gtg agc acc acc cgc gac ggc gtt 835Asp Gly Val Glu His Thr Trp Thr Val Ser Thr Thr Arg Asp Gly Val230 235 240 245gag tac ttc att gat gat gag gcg att aac cgc cag tca tgg cgc aac 883Glu Tyr Phe Ile Asp Asp Glu Ala Ile Asn Arg Gln Ser Trp Arg Asn
250 255 260gat gtc act ttg ggg cat gcc gaa att gat gat ttc ggg atc tcc gcg 931Asp Val Thr Leu Gly His Ala Glu Ile Asp Asp Phe Gly Ile Ser Ala
265 270 275cag acg ttt gat gag atc gtc gac cgc gaa tgg act ctc act ctt aat 979Gln Thr Phe Asp Glu Ile Val Asp Arg Glu Trp Thr Leu Thr Leu Asn
280 285 290caa aag gta gaa agc gcc gac tgg gca aaa cca cgt tcc tct gag gaa 1027Gln Lys Val Glu Ser Ala Asp Trp Ala Lys Pro Arg Ser Ser Glu Glu
295 300 305gat ttc cca gtc cgg tcc atg gtg att gac cgc atc gag gtc acc gga 1075Asp Phe Pro Val Arg Ser Met Val Ile Asp Arg Ile Glu Val Thr Gly310 315 320 325tct ccc gca gta tct gaa gac acc ccc atg cca gat acc acc cag ctt 1123Ser Pro Ala Val Ser Glu Asp Thr Pro Met Pro Asp Thr Thr Gln Leu
330 335 340ttg acc caa gac act ctg gaa tac ctc ggt cgc atg cca gtg ctg gaa 1171Leu Thr Gln Asp Thr Leu Glu Tyr Leu Gly Arg Met Pro Val Leu Glu
345 350 355cgc tac gag cca gca agt gct gat ttt gcc gat ggc cgc agg cct tcc 1219Arg Tyr Glu Pro Ala Ser Ala Asp Phe Ala Asp Gly Arg Arg Pro Ser
360 365 370tgg aac tac ttc aat ttg aag gaa tcg tgg cag aat cca gaa ctc gag 1267Trp Asn Tyr Phe Asn Leu Lys Glu Ser Trp Gln Asn Pro Glu Leu Glu
375 380 385caa cgc cca gaa gct gtc gaa ttc gtt gat gga cgc atg gat atc gtg 1315Gln Arg Pro Glu Ala Val Glu Phe Val Asp Gly Arg Met Asp Ile Val390 395 400 405acc cgt cgc cac tgt ctg gcc acc act gat gac atc gcc act ccg gaa 1363Thr Arg Arg His Cys Leu Ala Thr Thr Asp Asp Ile Ala Thr Pro Glu
410 415 420aac gca cag gag gaa ccg tgc gcg ccg ggt gag gtg aca cgc tac agc 1411Asn Ala Gln Glu Glu Pro Cys Ala Pro Gly Glu Val Thr Arg Tyr Ser
425 430 435tca gcg cgt gtc cac ctt cca gag atc ccc gcc ggc aac ttc cgg ctc 1459Ser Ala Arg Val His Leu Pro Glu Ile Pro Ala Gly Asn Phe Arg Leu
440 445 450acc gtg cgc gca cgg gcg cag tcc gaa gag ctt gtc gac ggc gtc cgc 1507Thr Val Arg Ala Arg Ala Gln Ser Glu Glu Leu Val Asp Gly Val Arg
455 460 465ccc gct atc tgg atg cag aac aat acc aac ttc tgt gct gac aac gat 1555Pro Ala Ile Trp Met Gln Asn Asn Thr Asn Phe Cys Ala Asp Asn Asp470 475 480 485gga cgc cct tat ggt gaa ctg gat att act gag ttc tac agc tct cgt 1603Gly Arg Pro Tyr Gly Glu Leu Asp Ile Thr Glu Phe Tyr Ser Ser Arg
490 495 500gtg aac acc cag tac tcg gca gta cac ctt gga tgt gct ggc aac cgc 1651Val Asn Thr Gln Tyr Ser Ala Val His Leu Gly Cys Ala Gly Asn Arg
505 510 515cca gag atg aag ctt cgc caa atg gaa atg gaa gag tcc atg ttt ggg 1699Pro Glu Met Lys Leu Arg Gln Met Glu Met Glu Glu Ser Met Phe Gly
520 525 530gat tgg cat gac tgg ggc gtc gaa gtc ttc gac ggc cag atc gta ttc 1747Asp Trp His Asp Trp Gly Val Glu Val Phe Asp Gly Gln Ile Val Phe
535 540 545acc att gac ggc aag gca gta act tcc tct ggc aaa gat gtc ttt ggc 1795Thr Ile Asp Gly Lys Ala Val Thr Ser Ser Gly Lys Asp Val Phe Gly550 555 560 565aac tct gtt acc cca gcc gct gca cct ctt cgc ccc gcg cac ttc aag 1843Asn Ser Val Thr Pro Ala Ala Ala Pro Leu Arg Pro Ala His Phe Lys
570 575 580ttg tcg gaa gag gaa tac cgt gaa gtc atc ggg cag cct tgg cac ctt 1891Leu Ser Glu Glu Glu Tyr Arg Glu Val Ile Gly Gln Pro Trp His Leu
585 590 595att ttg aac acc atg gtg gag cag tct ggc aaa gac agc tgg att aca 1939Ile Leu Asn Thr Met Val Glu Gln Ser Gly Lys Asp Ser Trp Ile Thr
600 605 610gcg gtc gac aat aac gag gcg ttc cca gaa cac cgc ttc caa att gac 1987Ala Val Asp Asn Asn Glu Ala Phe Pro Glu His Arg Phe Gln Ile Asp
615 620 625cat gtg gca gta gat atc gag tct gac tct gtg gac aat gta tgg cct 2035His Val Ala Val Asp Ile Glu Ser Asp Ser Val Asp Asn Val Trp Pro630 635 640 645gac gct gcg aat gaa atc cca gac aat gtt ggt att gaa gac tct gat 2083Asp Ala Ala Asn Glu Ile Pro Asp Asn Val Gly Ile Glu Asp Ser Asp
650 655 660gat ggc agc gac ctg gag gtt ggt tcg acc gga agc tct aca gct gag 2131Asp Gly Ser Asp Leu Glu Val Gly Ser Thr Gly Ser Ser Thr Ala Glu
665 670 675acc gtg agc tgg atc tcg ttg ttc acc gcg ttg agc tcg ctg gtc ttc 2179Thr Val Ser Trp Ile Ser Leu Phe Thr Ala Leu Ser Ser Leu Val Phe
680 685 690aca ctg gct ctc aat caa gaa gca ttg cag aat ttg att aat cag ttc 2227Thr Leu Ala Leu Asn Gln Glu Ala Leu Gln Asn Leu Ile Asn Gln Phe
695 700 705atg aga cag ttc aag taatccttgt gggcgctttg tct 2265Met Arg Gln Phe Lys710<210>464<211>714<212>PRT<213>谷氨酸棒杆菌<400>464Leu Lys Lys His Val Thr Ser Ala Val Thr Ala Val Val Thr Ala Phe1 5 10 15Ser Thr Ala Ala Leu Gly Leu Ser Ile Ala Val Ser Pro Ala Val Ala
20 25 30Gln Val Ala Asn Pro Ala Pro Asp Leu Ser Ala Pro Tyr Thr Trp Val
35 40 45Glu Glu Phe Asp Ser Glu Asp Ala Leu Lys Gly Trp Asn Ile Phe Arg
50 55 60Gln Pro Asp Tyr Gly Ser Asp Lys Val Leu Tyr Thr Glu Asp Ala Leu65 70 75 80Ser Ile Glu Asp Gly Lys Leu Thr Ile Thr Thr Gln Arg His Cys Val
85 90 95Asp Glu Asp Phe Ala Ile Ser Asp Pro Val Asn Arg Gly Lys Leu Asn
100 105 110Asp Ser Thr Ala Gln Val Glu Pro Cys Ala Pro Gly Gln Phe Glu Lys
115 120 125Phe Thr Ser Ala Arg Ile Val Thr Pro Lys Ile Ala Arg Gly Glu Phe
130 135 140Asp Leu Ser Val Thr Ala Thr Leu Asn Thr Gly Gly Val Glu Gly Val145 150 155 160Arg Ser Ala Ile Trp Met Gln Asn Gly Glu Gln Ala Cys Ser Ser Ala
165 170 175Thr Asn Asn Gly Leu Tyr Gly Glu Leu Asp Leu Val Glu His Phe Ser
180 185 190Tyr Asp Leu Arg Ser Pro Trp Ser Pro Ser Asn Thr His Leu Gly Cys
195 200 205Asp Pro Glu Ser Val Asn Gly Thr Asn Arg Ala Pro Arg Glu Leu Lys
210 215 220Leu Asp Glu Ser Leu Asp Gly Val Glu His Thr Trp Thr Val Ser Thr225 230 235 240Thr Arg Asp Gly Val Glu Tyr Phe Ile Asp Asp Glu Ala Ile Asn Arg
245 250 255Gln Ser Trp Arg Asn Asp Val Thr Leu Gly His Ala Glu Ile Asp Asp
260 265 270Phe Gly Ile Ser Ala Gln Thr Phe Asp Glu Ile Val Asp Arg Glu Trp
275 280 285Thr Leu Thr Leu Asn Gln Lys Val Glu Ser Ala Asp Trp Ala Lys Pro
290 295 300Arg Ser Ser Glu Glu Asp Phe Pro Val Arg Ser Met Val Ile Asp Arg305 310 315 320Ile Glu Val Thr Gly Ser Pro Ala Val Ser Glu Asp Thr Pro Met Pro
325 330 335Asp Thr Thr Gln Leu Leu Thr Gln Asp Thr Leu Glu Tyr Leu Gly Arg
340 345 350Met Pro Val Leu Glu Arg Tyr Glu Pro Ala Ser Ala Asp Phe Ala Asp
355 360 365Gly Arg Arg Pro Ser Trp Asn Tyr Phe Asn Leu Lys Glu Ser Trp Gln
370 375 380Asn Pro Glu Leu Glu Gln Arg Pro Glu Ala Val Glu Phe Val Asp Gly385 390 395 400Arg Met Asp Ile Val Thr Arg Arg His Cys Leu Ala Thr Thr Asp Asp
405 410 415Ile Ala Thr Pro Glu Asn Ala Gln Glu Glu Pro Cys Ala Pro Gly Glu
420 425 430Val Thr Arg Tyr Ser Ser Ala Arg Val His Leu Pro Glu Ile Pro Ala
435 440 445Gly Asn Phe Arg Leu Thr Val Arg Ala Arg Ala Gln Ser Glu Glu Leu
450 455 460Val Asp Gly Val Arg Pro Ala Ile Trp Met Gln Asn Asn Thr Asn Phe465 470 475 480Cys Ala Asp Asn Asp Gly Arg Pro Tyr Gly Glu Leu Asp Ile Thr Glu
485 490 495Phe Tyr Ser Ser Arg Val Asn Thr Gln Tyr Ser Ala Val His Leu Gly
500 505 510Cys Ala Gly Asn Arg Pro Glu Met Lys Leu Arg Gln Met Glu Met Glu
515 520 525Glu Ser Met Phe Gly Asp Trp His Asp Trp Gly Val Glu Val Phe Asp
530 535 540Gly Gln Ile Val Phe Thr Ile Asp Gly Lys Ala Val Thr Ser Ser Gly545 550 555 560Lys Asp Val Phe Gly Asn Ser Val Thr Pro Ala Ala Ala Pro Leu Arg
565 570 575Pro Ala His Phe Lys Leu Ser Glu Glu Glu Tyr Arg Glu Val Ile Gly
580 585 590Gln Pro Trp His Leu Ile Leu Asn Thr Met Val Glu Gln Ser Gly Lys
595 600 605Asp Ser Trp Ile Thr Ala Val Asp Asn Asn Glu Ala Phe Pro Glu His
610 615 620Arg Phe Gln Ile Asp His Val Ala Val Asp Ile Glu Ser Asp Ser Val625 630 635 640Asp Asn Val Trp Pro Asp Ala Ala Asn Glu Ile Pro Asp Asn Val Gly
645 650 655Ile Glu Asp Ser Asp Asp Gly Ser Asp Leu Glu Val Gly Ser Thr Gly
660 665 670Ser Ser Thr Ala Glu Thr Val Ser Trp Ile Ser Leu Phe Thr Ala Leu
675 680 685Ser Ser Leu Val Phe Thr Leu Ala Leu Asn Gln Glu Ala Leu Gln Asn
690 695 700Leu Ile Asn Gln Phe Met Arg Gln Phe Lys705 710<210>465<211>389<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(382)<223>RXN03015<400>465ccgggtttgc ctgaggtttt ggagcgcaat ctggaaaatg gtcgcctgaa cttcactact 60gattatgcgg aggctgcggc tttcgcgcag gtgcatttct ttg ggc gtg ggt act 115
Leu Gly Val Gly Thr
1 5cct cag cag aag ggt act tat gcg gcg gat ctg acg tat gtt cgt cag 163Pro Gln Gln Lys Gly Thr Tyr Ala Ala Asp Leu Thr Tyr Val Arg Gln
10 15 20gtt gtt gag gat ttg gtg ccg ctg ctt gag ggc gag cac att att ttc 211Val Val Glu Asp Leu Val Pro Leu Leu Glu Gly Glu His Ile Ile Phe
25 30 35ggc aag tct acg gtt ccg gtt ggt act gct gag cag ttg cag gag ctt 259Gly Lys Ser Thr Val Pro Val Gly Thr Ala Glu Gln Leu Gln Glu Leu
40 45 50gct gat tct ctg gtc aag cct ggt tcg cac gtg gag atc gcg tgg aat 307Ala Asp Ser Leu Val Lys Pro Gly Ser His Val Glu Ile Ala Trp Asn
55 60 65ccg gag ttc ttg cgt gag ggc tac gcg gtc aaa gac acc atc act ccg 355Pro Glu Phe Leu Arg Glu Gly Tyr Ala Val Lys Asp Thr Ile Thr Pro70 75 80 85gac cgc atc gtg gtg ggt gtg cgt gaa taagaca 389Asp Arg Ile Val Val Gly Val Arg Glu
90<210>466<211>94<212>PRT<213>谷氨酸棒杆菌<400>466Leu Gly Val Gly Thr Pro Gln Gln Lys Gly Thr Tyr Ala Ala Asp Leu1 5 10 15Thr Tyr Val Arg Gln Val Val Glu Asp Leu Val Pro Leu Leu Glu Gly
20 25 30Glu His Ile Ile Phe Gly Lys Ser Thr Val Pro Val Gly Thr Ala Glu
35 40 45Gln Leu Gln Glu Leu Ala Asp Ser Leu Val Lys Pro Gly Ser His Val
50 55 60Glu Ile Ala Trp Asn Pro Glu Phe Leu Arg Glu Gly Tyr Ala Val Lys65 70 75 80Asp Thr Ile Thr Pro Asp Arg Ile Val Val Gly Val Arg Glu
85 90<210>467<211>801<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(778)<223>RXN03056<400>467ttactatcac tggttttgca gatgagatcg cacatgatct cgacgaacag atttccttac 60ttaataaact gaagaacagc tcgcggaagc taaagccaaa ttg gat gcc gca gga 115
Leu Asp Ala Ala Gly
1 5att tca ctt tca gcc gtt gga tcc gac ttc ggc aag atc aac atc acc 163Ile Ser Leu Ser Ala Val Gly Ser Asp Phe Gly Lys Ile Asn Ile Thr
10 15 20gat cct ttt gaa gat cac ttg gag cgc gca cgc cac ggt gtt gag gtc 211Asp Pro Phe Glu Asp His Leu Glu Arg Ala Arg His Gly Val Glu Val
25 30 35gct aag ctc ttc ggc gcg aaa tac atc cgc atg ttc tcc ttc ttt att 259Ala Lys Leu Phe Gly Ala Lys Tyr Ile Arg Met Phe Ser Phe Phe Ile
40 45 50gca gaa ggc gac aac cct gaa agc ttc cgc aaa gaa gta ctc tcc cgc 307Ala Glu Gly Asp Asn Pro Glu Ser Phe Arg Lys Glu Val Leu Ser Arg
55 60 65acc cac gca atg gtc gaa ctc gca gaa gcc ggc ggc atc acc ctc ctc 355Thr His Ala Met Val Glu Leu Ala Glu Ala Gly Gly Ile Thr Leu Leu70 75 80 85cac gaa aat gaa aag gga atc tat ggc gac tcc ccg cag cgc gtg aag 403His Glu Asn Glu Lys Gly Ile Tyr Gly Asp Ser Pro Gln Arg Val Lys
90 95 100gat tta atc acc agc atc gac tcc cct aac tac cgc gca atc tac gac 451Asp Leu Ile Thr Ser Ile Asp Ser Pro Asn Tyr Arg Alg Ile Tyr Asp
105 110 115gca gct aac tac gtg caa acc gga ttc aag cct ttt gat gag gca tgg 499Ala Ala Asn Tyr Val Gln Thr Gly Phe Lys Pro Phe Asp Glu Ala Trp
120 125 130ccg atc gtt aag gac tac gtc gac tac gtc cac atc aag gac gcg acc 547Pro Ile Val Lys Asp Tyr Val Asp Tyr Val His Ile Lys Asp Ala Thr
135 140 145att cca gat gca gag cac ccc atc gga atc atc aag cca gca gga caa 595Ile Pro Asp Ala Glu His Pro Ile Gly Ile Ile Lys Pro Ala Gly Gln150 155 160 165ggc gac ggc caa tac cca gag ctc ctt gcc gcg cta aac gcc gac ggt 643Gly Asp Gly Gln Tyr Pro Glu Leu Leu Ala Ala Leu Asn Ala Asp Gly
170 175 180tac aac gga ttc gtc tcc atc gag cct cac ctg ggt gac ttc gat gaa 691Tyr Asn Gly Phe Val Ser Ile Glu Pro His Leu Gly Asp Phe Asp Glu
185 190 195ttc ggc gga ctc tgc gga cct gac ctg tgg acc agc gca tgc gac gct 739Phe Gly Gly Leu Cys Gly Pro Asp Leu Trp Thr Ser Ala Cys Asp Ala
200 205 210ctc gca gga atc ctg aac aac atc aac gcc gag tac aac taaggacaac 788Leu Ala Gly Ile Leu Asn Asn Ile Asn Ala Glu Tyr Asn
215 220 225tgataatgac aaa 801<210>468<211>226<212>PRT<213>谷氨酸棒杆菌<400>468Leu Asp Ala Ala Gly Ile Ser Leu Ser Ala Val Gly Ser Asp Phe Gly1 5 10 15Lys Ile Asn Ile Thr Asp Pro Phe Glu Asp His Leu Glu Arg Ala Arg
20 25 30His Gly Val Glu Val Ala Lys Leu Phe Gly Ala Lys Tyr Ile Arg Met
35 40 45Phe Ser Phe Phe Ile Ala Glu Gly Asp Asn Pro Glu Ser Phe Arg Lys
50 55 60Glu Val Leu Ser Arg Thr His Ala Met Val Glu Leu Ala Glu Ala Gly65 70 75 80Gly Ile Thr Leu Leu His Glu Asn Glu Lys Gly Ile Tyr Gly Asp Ser
85 90 95Pro Gln Arg Val Lys Asp Leu Ile Thr Ser Ile Asp Ser Pro Asn Tyr
100 105 110Arg Ala Ile Tyr Asp Ala Ala Asn Tyr Val Gln Thr Gly Phe Lys Pro
115 120 125Phe Asp Glu Ala Trp Pro Ile Val Lys Asp Tyr Val Asp Tyr Val His
130 135 140Ile Lys Asp Ala Thr Ile Pro Asp Ala Glu His Pro Ile Gly Ile Ile145 150 155 160Lys Pro Ala Gly Gln Gly Asp Gly Gln Tyr Pro Glu Leu Leu Ala Ala
165 170 175Leu Asn Ala Asp Gly Tyr Asn Gly Phe Val Ser Ile Glu Pro His Leu
180 185 190Gly Asp Phe Asp Glu Phe Gly Gly Leu Cys Gly Pro Asp Leu Trp Thr
195 200 205Ser Ala Cys Asp Ala Leu Ala Gly Ile Leu Asn Asn Ile Asn Ala Glu
210 215 220Tyr Asn225<210>469<211>687<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(664)<223>RXN03030<400>469gaagatgaag cagaaaagat cattggtgcg ccagaggttt ctgcattggg caacaaagca 60cagcttgatt ccgtcacctt gctgcgtaac aaccccatcc gtg ctg cca ctg gat 115
Val Leu Pro Leu Asp
1 5cct gca gca agc ctg aag atc tac cca ttg gtt act ggc cgt acc aag 163Pro Ala Ala Ser Leu Lys Ile Tyr Pro Leu Val Thr Gly Arg Thr Lys
10 15 20atc gac gag gtt caa cta cag ctg gaa gca gcc att cgc gca gaa ctc 211Ile Asp Glu Val Gln Leu Gln Leu Glu Ala Ala Ile Arg Ala Glu Leu
25 30 35cca ggg gta acc ttg gtg tct tcc gag tca gaa gca gat ctt gca atc 259Pro Gly Val Thr Leu Val Ser Ser Glu Ser Glu Ala Asp Leu Ala Ile
40 45 50gtg tgg gct cgg cct gaa att gca ctg ttt gaa gat gac ctc gaa ggt 307Val Trp Ala Arg Pro Glu Ile Ala Leu Phe Glu Asp Asp Leu Glu Gly
55 60 65gtt tcc ctc tct gtt gac cct cgt gcc aat ggt gtc gat gtg gaa cgc 355Val Ser Leu Ser Val Asp Pro Arg Ala Asn Gly Val Asp Val Glu Arg70 75 80 85gtt cag gct gtg gaa gct gca gtc cca acc atc ttg gct gtg aac ttc 403Val Gln Ala Val Glu Ala Ala Val Pro Thr Ile Leu Ala Val Asn Phe
90 95 100acc aac cct tgg gtg ctg tct gag atc gag cct ggt gcc gct gcc gtg 451Thr Asn Pro Trp Val Leu Ser Glu Ile Glu Pro Gly Ala Ala Ala Val
105 110 115gtg ggc act ttt gag atc aag cca gag ttc ctt ctc aag gct ttg act 499Val Gly Thr Phe Glu Ile Lys Pro Glu Phe Leu Leu Lys Ala Leu Thr
120 125 130ggt caa gag gga gga cca aag ggc aag ctg cca ttg act gtt cct gct 547Gly Gln Glu Gly Gly Pro Lys Gly Lys Leu Pro Leu Thr Val Pro Ala
135 140 145tcc atg cag gcg att gct gat tcc cct cgc gat atc cca ggc aag ttc 595Ser Met Gln Ala Ile Ala Asp Ser Pro Arg Asp Ile Pro Gly Lys Phe150 155 160 165ctc gat gag tct tac acc tac gtg gac tcc gca ggg atg gcc tac aag 643Leu Asp Glu Ser Tyr Thr Tyr Val Asp Ser Ala Gly Met Ala Tyr Lys
170 175 180tac ggt cac gga ctt aat ttc tagattgtag gtagtctcgt ggg 687Tyr Gly His Gly Leu Asn Phe
185<210>470<211>188<212>PRT<213>谷氨酸棒杆菌<400>470Val Leu Pro Leu Asp Pro Ala Ala Ser Leu Lys Ile Tyr Pro Leu Val1 5 10 15Thr Gly Arg Thr Lys Ile Asp Glu Val Gln Leu Gln Leu Glu Ala Ala
20 25 30Ile Arg Ala Glu Leu Pro Gly Val Thr Leu Val Ser Ser Glu Ser Glu
35 40 45Ala Asp Leu Ala Ile Val Trp Ala Arg Pro Glu Ile Ala Leu Phe Glu
50 55 60Asp Asp Leu Glu Gly Val Ser Leu Ser Val Asp Pro Arg Ala Asn Gly65 70 75 80Val Asp Val Glu Arg Val Gln Ala Val Glu Ala Ala Val Pro Thr Ile
85 90 95Leu Ala Val Asn Phe Thr Asn Pro Trp Val Leu Ser Glu Ile Glu Pro
100 105 110Gly Ala Ala Ala Val Val Gly Thr Phe Glu Ile Lys Pro Glu Phe Leu
115 120 125Leu Lys Ala Leu Thr Gly Gln Glu Gly Gly Pro Lys Gly Lys Leu Pro
130 135 140Leu Thr Val Pro Ala Ser Met Gln Ala Ile Ala Asp Ser Pro Arg Asp145 150 155 160Ile Pro Gly Lys Phe Leu Asp Glu Ser Tyr Thr Tyr Val Asp Ser Ala
165 170 175Gly Met Ala Tyr Lys Tyr Gly His Gly Leu Asn Phe
180 185<210>471<21l>1062<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1039)<223>RXN00401<400>471cttttaagca tttcaacatg ccaaactaat cttggacagg tatcactggc tcctcttaga 60ctccaaagtg tcttagaaaa cactcaccca aggagccctc atg gca cgc ttt tca 115
Met Ala Arg Phe Ser
1 5cca caa gat ctc gca gac cac ctc aag gat gga ctg ctc tct ttc ccg 163Pro Gln Asp Leu Ala Asp His Leu Lys Asp Gly Leu Leu Ser Phe Pro
10 15 20gcc acc gct ttc caa gat gac ctc gaa gta gat gaa gct gct tat gtc 211Ala Thr Ala Phe Gln Asp Asp Leu Glu Val Asp Glu Ala Ala Tyr Val
25 30 35gag cac att gaa tgg cag tcc agc tac cca gtc gcc ggc ctc ttc gca 259Glu His Ile Glu Trp Gln Ser Ser Tyr Pro Val Ala Gly Leu Phe Ala
40 45 50gct ggc ggt act ggc gaa gga ttc agc ctt acc gtt gag gaa aac cac 307Ala Gly Gly Thr Gly Glu Gly Phe Ser Leu Thr Val Glu Glu Asn His
55 60 65cgt gtc act caa ctt gca gtt cag gcg tcc agc ccg gaa gtt ccc gtg 355Arg Val Thr Gln Leu Ala Val Gln Ala Ser Ser Pro Glu Val Pro Val70 75 80 85ttg ggg tct gct act ggc tca act aag tct gcc atc gca aac gca cag 403Leu Gly Ser Ala Thr Gly Ser Thr Lys Ser Ala Ile Ala Asn Ala Gln
90 95 100ggc gca gag gca gca ggc gct gaa ggt gtc ctc ctg ctt cct ccc tac 451Gly Ala Glu Ala Ala Gly Ala Glu Gly Val Leu Leu Leu Pro Pro Tyr
105 110 115ctc acc gaa tgc gac gca gaa ggc ctg tac aac cat gca gcc gca gtc 499Leu Thr Glu Cys Asp Ala Glu Gly Leu Tyr Asn His Ala Ala Ala Val
120 125 130tgt gaa tcc act tct ctt ggt gtc atc gtg tac aac cgt gcc aat gcc 547Cys Glu Ser Thr Ser Leu Gly Val Ile Val Tyr Asn Arg Ala Asn Ala
135 140 145atc tac tcc cca gag gtt atc gct cga ctc tct gag cgc tac ccc aac 595Ile Tyr Ser Pro Glu Val Ile Ala Arg Leu Ser Glu Arg Tyr Pro Asn150 155 160 165ttc att gga ttt aaa gat ggc acc gga aac atc gag cac cta gca aag 643Phe Ile Gly Phe Lys Asp Gly Thr Gly Asn Ile Glu His Leu Ala Lys
170 175 180atc acc acg cta tgc gga gat cgc ctg ttc tac ctc ggt gga ctt ccc 691Ile Thr Thr Leu Cys Gly Asp Arg Leu Phe Tyr Leu Gly Gly Leu Pro
185 190 195acc gct gag acc ttt gca cta cca ctg ctt cag atg ggc atg agc acc 739Thr Ala Glu Thr Phe Ala Leu Pro Leu Leu Gln Met Gly Met Ser Thr
200 205 210tac tcc tct gca atg ttc aac ttc att cca gat ttc gca ctg agc ttc 787Tyr Ser Ser Ala Met Phe Asn Phe Ile Pro Asp Phe Ala Leu Ser Phe
215 220 225tac gcc gat gtt cgt gcg cag gac agc gca gca gta aag cag aag ctg 835Tyr Ala Asp Val Arg Ala Gln Asp Ser Ala Ala Val Lys Gln Lys Leu230 235 240 245agc gat ttt gtg ctc ccc tac ttg gat atc cgc gat cgc gca caa ggc 883Ser Asp Phe Val Leu Pro Tyr Leu Asp Ile Arg Asp Arg Ala Gln Gly
250 255 260tac ggt gtc tcc att ggt aag ggc gga ctc aag gct gtt ggc cgc aac 931Tyr Gly Val Ser Ile Gly Lys Gly Gly Leu Lys Ala Val Gly Arg Asn
265 270 275gct ggc ggc gtt cgc cca cca ctg cgt aac ctt tcc gag caa gat atc 979Ala Gly Gly Val Arg Pro Pro Leu Arg Asn Leu Ser Glu Gln Asp Ile
280 285 290gcg gac ctg tcg gat ttg ctt gcc acc tct ggc gca ggt tcc tac cgc 1027Ala Asp Leu Ser Asp Leu Leu Ala Thr Ser Gly Ala Gly Ser Tyr Arg
295 300 305ctt cag ttg agg tgaaagcatg atcaccgcaa ccg 1062Leu Gln Leu Arg310<210>472<211>313<212>PRT<213>谷氨酸棒杆菌<400>472Met Ala Arg Phe Ser Pro Gln Asp Leu Ala Asp His Leu Lys Asp Gly1 5 10 15Leu Leu Ser Phe Pro Ala Thr Ala Phe Gln Asp Asp Leu Glu Val Asp
20 25 30Glu Ala Ala Tyr Val Glu His Ile Glu Trp Gln Ser Ser Tyr Pro Val
35 40 45Ala Gly Leu Phe Ala Ala Gly Gly Thr Gly Glu Gly Phe Ser Leu Thr
50 55 60Val Glu Glu Asn His Arg Val Thr Gln Leu Ala Val Gln Ala Ser Ser65 70 75 80Pro Glu Val Pro Val Leu Gly Ser Ala Thr Gly Ser Thr Lys Ser Ala
85 90 95Ile Ala Asn Ala Gln Gly Ala Glu Ala Ala Gly Ala Glu Gly Val Leu
100 105 110Leu Leu Pro Pro Tyr Leu Thr Glu Cys Asp Ala Glu Gly Leu Tyr Asn
115 120 125His Ala Ala Ala Val Cys Glu Ser Thr Ser Leu Gly Val Ile Val Tyr
130 135 140Asn Arg Ala Asn Ala Ile Tyr Ser Pro Glu Val Ile Ala Arg Leu Ser145 150 155 160Glu Arg Tyr Pro Asn Phe Ile Gly Phe Lys Asp Gly Thr Gly Asn Ile
165 170 175Glu His Leu Ala Lys Ile Thr Thr Leu Cys Gly Asp Arg Leu Phe Tyr
180 185 190Leu Gly Gly Leu Pro Thr Ala Glu Thr Phe Ala Leu Pro Leu Leu Gln
195 200 205Met Gly Met Ser Thr Tyr Ser Ser Ala Met Phe Asn Phe Ile Pro Asp
210 215 220Phe Ala Leu Ser Phe Tyr Ala Asp Val Arg Ala Gln Asp Ser Ala Ala225 230 235 240Val Lys Gln Lys Leu Ser Asp Phe Val Leu Pro Tyr Leu Asp Ile Arg
245 250 255Asp Arg Ala Gln Gly Tyr Gly Val Ser Ile Gly Lys Gly Gly Leu Lys
260 265 270Ala Val Gly Arg Asn Ala Gly Gly Val Arg Pro Pro Leu Arg Asn Leu
275 280 285Ser Glu Gln Asp Ile Ala Asp Leu Ser Asp Leu Leu Ala Thr Ser Gly
290 295 300Ala Gly Ser Tyr Arg Leu Gln Leu Arg305 310<210>473<211>924<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(901)<223>RXN02125<400>473gacctaaaat tcatcacctc accgttttta aggcttagaa aaatagcagt gttgggatgt 60gaatatccat ttatgctgct gtagtcggct atgtggacgc atg gtg gca acc tct 115
Met Val Ala Thr Ser
1 5cag ttt atc gat gac agc gag gct gcc cag gcg gta cgc gca gct att 163Gln Phe Ile Asp Asp Ser Glu Ala Ala Gln Ala Val Arg Ala Ala Ile
10 15 20gtt gca gga tac cga aac att gat act gcc cta gcg tat gga aac gag 211Val Ala Gly Tyr Arg Asn Ile Asp Thr Ala Leu Ala Tyr Gly Asn Glu
25 30 35cgc ggc gtt ggc gaa ggc att cgc acc gct gga gtg ccc cgc gag gag 259Arg Gly Val Gly Glu Gly Ile Arg Thr Ala Gly Val Pro Arg Glu Glu
40 45 50ctc ttt att tcc acc aag cta gct gca gaa atc aaa gat tac gat gga 307Leu Phe Ile Ser Thr Lys Leu Ala Ala Glu Ile Lys Asp Tyr Asp Gly
55 60 65gca gtc gcc gcg att gat gag tct ttg gcg aaa att ggc ttg gat tat 355Ala Val Ala Ala Ile Asp Glu Ser Leu Ala Lys Ile Gly Leu Asp Tyr70 75 80 85gtc gat ctg atg ctc att cac tcc cca caa cca tgg agt gat ttc cgt 403Val Asp Leu Met Leu Ile His Ser Pro Gln Pro Trp Ser Asp Phe Arg
90 95 100ggt ggg gac tat tca gag gga aac cgt gaa gcg tgg cgc gcg ctg gaa 451Gly Gly Asp Tyr Ser Glu Gly Asn Arg Glu Ala Trp Arg Ala Leu Glu
105 110 115gat gcc tac aaa gcc gga aag att cga tcc att ggt gtc tcg aac ttc 499Asp Ala Tyr Lys Ala Gly Lys Ile Arg Ser Ile Gly Val Ser Asn Phe
120 125 130ctg gag gcc gat ctg gag aat atc tta gac tcc gcg acg gtt gct cct 547Leu Glu Ala Asp Leu Glu Asn Ile Leu Asp Ser Ala Thr Val Ala Pro
135 140 145cac gtt aat cag ctt ctt gtg cat gtt gga aac acc cca agc gag tta 595His Val Asn Gln Leu Leu Val His Val Gly Asn Thr Pro Ser Glu Leu150 155 160 165atc agt ttc tgc gat tcc aag ggc att ctg gtc gaa gca tat tca ccc 643Ile Ser Phe Cys Asp Ser Lys Gly Ile Leu Val Glu Ala Tyr Ser Pro
170 175 180atc gcc cac gga gag atg ctg aag aac cag cag gtc aag gcg att gct 691Ile Ala His Gly Glu Met Leu Lys Asn Gln Gln Val Lys Ala Ile Ala
185 190 195gac aag tac aac gtg agc att ccg cag cta tgc att cgg tac aca att 739Asp Lys Tyr Asn Val Ser Ile Pro Gln Leu Cys Ile Arg Tyr Thr Ile
200 205 210caa ctg gga acg gtg tct ttg cca aag act gcc aac cca gat cat atg 787Gln Leu Gly Thr Val Ser Leu Pro Lys Thr Ala Asn Pro Asp His Met
215 220 225agc tcc aat gcg cag atc gac ttt gaa att tcc gag gaa gac atg gcg 835Ser Ser Asn Ala Gln Ile Asp Phe Glu Ile Ser Glu Glu Asp Met Ala230 235 240 245gca ctt caa gaa gtg acc gcc cgc gat tat ggc gag cac agc ggt ttt 883Ala Leu Gln Glu Val Thr Ala Arg Asp Tyr Gly Glu His Ser Gly Phe
250 255 260cct gtg tat tcc ggc aag tagaaagatt tttatcatgg gac 924Pro Val Tyr Ser Gly Lys
265<210>474<211>267<212>PRT<213>谷氨酸棒杆菌<400>474Met Val Ala Thr Ser Gln Phe Ile Asp Asp Ser Glu Ala Ala Gln Ala1 5 10 15Val Arg Ala Ala Ile Val Ala Gly Tyr Arg Asn Ile Asp Thr Ala Leu
20 25 30Ala Tyr Gly Asn Glu Arg Gly Val Gly Glu Gly Ile Arg Thr Ala Gly
35 40 45Val Pro Arg Glu Glu Leu Phe Ile Ser Thr Lys Leu Ala Ala Glu Ile
50 55 60Lys Asp Tyr Asp Gly Ala Val Ala Ala Ile Asp Glu Ser Leu Ala Lys65 70 75 80Ile Gly Leu Asp Tyr Val Asp Leu Met Leu Ile His Ser Pro Gln Pro
85 90 95Trp Ser Asp Phe Arg Gly Gly Asp Tyr Ser Glu Gly Asn Arg Glu Ala
100 105 110Trp Arg Ala Leu Glu Asp Ala Tyr Lys Ala Gly Lys Ile Arg Ser Ile
115 120 125Gly Val Ser Asn Phe Leu Glu Ala Asp Leu Glu Asn Ile Leu Asp Ser
130 135 140Ala Thr Val Ala Pro His Val Asn Gln Leu Leu Val His Val Gly Asn145 150 155 160Thr Pro Ser Glu Leu Ile Ser Phe Cys Asp Ser Lys Gly Ile Leu Val
165 170 175Glu Ala Tyr Ser Pro Ile Ala His Gly Glu Met Leu Lys Asn Gln Gln
180 185 190Val Lys Ala Ile Ala Asp Lys Tyr Asn Val Ser Ile Pro Gln Leu Cys
195 200 205Ile Arg Tyr Thr Ile Gln Leu Gly Thr Val Ser Leu Pro Lys Thr Ala
210 215 220Asn Pro Asp His Met Ser Ser Asn Ala Gln Ile Asp Phe Glu Ile Ser225 230 235 240Glu Glu Asp Met Ala Ala Leu Gln Glu Val Thr Ala Arg Asp Tyr Gly
245 250 255Glu His Ser Gly Phe Pro Val Tyr Ser Gly Lys
260 265<210>475<211>3561<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(3538)<223>RXN00200<400>475aacccggagt catttgatca gatgtggcag accaagcaag tgggaccttt cgtggtggta 60acgcacaatg agtaattcct caccaaacga cccaagccct atg cgc caa gtc ggt 115
Met Arg Gln Val Gly
1 5ggt aat ggg ggc cat caa cta gac tcg atc aac gtg tca gat gta gtt 163Gly Asn Gly Gly His Gln Leu Asp Ser Ile Asn Val Ser Asp Val Val
10 15 20gag tcg aag aaa cta aag ggt tct gcg caa gaa ccc ccg cag gtt gcc 211Glu Ser Lys Lys Leu Lys Gly Ser Ala Gln Glu Pro Pro Gln Val Ala
25 30 35ccg ggc tgg ctg aag aaa ctg gct atc tca tca ggt ctg ctt ggc ctg 259Pro Gly Trp Leu Lys Lys Leu Ala Ile Ser Ser Gly Leu Leu Gly Leu
40 45 50ttg atg ttt gtg ctg ttg cct ttc ctg cca gtg aac cag gtg cag tct 307Leu Met Phe Val Leu Leu Pro Phe Leu Pro Val Asn Gln Val Gln Ser
55 60 65tcg ctg tca tgg cca caa aat ggt gag ctt tcc agc gtt aac gcc ccg 355Ser Leu Ser Trp Pro Gln Asn Gly Glu Leu Ser Ser Val Asn Ala Pro70 75 80 85ctg att tcc tac gca ccg cag tcg atg gat gcg tcc atc cct gtg tcc 403Leu Ile Ser Tyr Ala Pro Gln Ser Met Asp Ala Ser Ile Pro Val Ser
90 95 100gcg ctg gac agt ctc aat gac aat cag tcg ttg gtg atg ggc acg ttg 451Ala Leu Asp Ser Leu Asn Asp Asn Gln Ser Leu Val Met Gly Thr Leu
105 110 115cct ctg gac agt acg gac gcc acc aac cgt ggt ctg ttt gtg cgc acc 499Pro Leu Asp Ser Thr Asp Ala Thr Asn Arg Gly Leu Phe Val Arg Thr
120 125 130atc gac ggt aac ctt gac gtg att gtt cgc ggt gag gtg ctg ttg gat 547Ile Asp Gly Asn Leu Asp Val Ile Val Arg Gly Glu Val Leu Leu Asp
135 140 145ctt tca cca aca gag gtg aac cgt ctg cca gat gat gcg atc cta gag 595Leu Ser Pro Thr Glu Val Asn Arg Leu Pro Asp Asp Ala Ile Leu Glu150 155 160 165att tcc tcc acc gag gaa acc acc agc gcg gaa atc acc ggc acg gca 643Ile Ser Ser Thr Glu Glu Thr Thr Ser Ala Glu Ile Thr Gly Thr Ala
170 175 180ttc agc ggc gag acc gaa ggc gat gag cgg cct cag gtc acc ggc gtt 691Phe Ser Gly Glu Thr Glu Gly Asp Glu Arg Pro Gln Val Thr Gly Val
185 190 195tac acc gag ctt gtc gac gac ccc tcc acc gca tcg gcc ctg gcc tca 739Tyr Thr Glu Leu Val Asp Asp Pro Ser Thr Ala Ser Ala Leu Ala Ser
200 205 210gcg ggc tta aac gtt gat att gag atc aac tcc cgc ttc act tca tcc 787Ala Gly Leu Asn Val Asp Ile Glu Ile Asn Ser Arg Phe Thr Ser Ser
215 220 225ccc agc ctt cta aag tac gca gcc atc ttc att ggc ctt gcg tct gtg 835Pro Ser Leu Leu Lys Tyr Ala Ala Ile Phe Ile Gly Leu Ala Ser Val230 235 240 245ttg gtc tcc ctg tgg aca ctg cac cgc atg gat att ttg gat ggt cga 883Leu Val Ser Leu Trp Thr Leu His Arg Met Asp Ile Leu Asp Gly Arg
250 255 260aaa gca cac cgc ttc ctg cct gcc aac tgg tac aag ctg aag cca ctt 931Lys Ala His Arg Phe Leu Pro Ala Asn Trp Tyr Lys Leu Lys Pro Leu
265 270 275gat ggt gtt gtc gta gcg att ttg gtg ttc tgg cac ttc ctt ggc gcc 979Asp Gly Val Val Val Ala Ile Leu Val Phe Trp His Phe Leu Gly Ala
280 285 290aac acc tct gac gac ggc ttc atc atg acc atg gcc cgc gtg tcc cag 1027Asn Thr Ser Asp Asp Gly Phe Ile Met Thr Met Ala Arg Val Ser Gln
295 300 305aac gcg gat tat atg gcc aac tac tac cgc tgg ttc ggt gtc cca gaa 1075Asn Ala Asp Tyr Met Ala Asn Tyr Tyr Arg Trp Phe Gly Val Pro Glu310 315 320 325tca cca ttc ggc gca cca tat tac gac ttg ctg gct ctg atg gcc tac 1123Ser Pro Phe Gly Ala Pro Tyr Tyr Asp Leu Leu Ala Leu Met Ala Tyr
330 335 340atc tca acc tca tca atc tgg ctt cgt cta ccc gca ttg ctc gct gga 1171Ile Ser Thr Ser Ser Ile Trp Leu Arg Leu Pro Ala Leu Leu Ala Gly
345 350 355ctg atc atg tgg ttc gtg atc acc aga gag gtc atg cca cgg ttt ggc 1219Leu Ile Met Trp Phe Val Ile Thr Arg Glu Val Met Pro Arg Phe Gly
360 365 370tca ttg gtt aac ggt cgc cgc gtt gcg cac tgg tct gca gcc atg gtg 1267Ser Leu Val Asn Gly Arg Arg Val Ala His Trp Ser Ala Ala Met Val
375 380 385ttc ctg gcg ttc tgg ctt cca tac aac aac ggc act cgc cca gag cca 1315Phe Leu Ala Phe Trp Leu Pro Tyr Asn Asn Gly Thr Arg Pro Glu Pro390 395 400 405atc atc gcg atg gga gct cta ctt gcg tgg gtt tcc ttc gag cgc gct 1363Ile Ile Ala Met Gly Ala Leu Leu Ala Trp Val Ser Phe Glu Arg Ala
410 415 420atc gct acc tcc agg ttg ttg ccc gct gcc att ggt gtc att atc gcc 1411Ile Ala Thr Ser Arg Leu Leu Pro Ala Ala Ile Gly Val Ile Ile Ala
425 430 435acc att tcc ctg gca tca ggc ccc acc ggt cta atg gca gtt gct gcg 1459Thr Ile Ser Leu Ala Ser Gly Pro Thr Gly Leu Met Ala Val Ala Ala
440 445 450ttg ctg gtc agt ttg tcc gcg ttg att cgc att ctt tat agg cgc ttg 1507Leu Leu Val Ser Leu Ser Ala Leu Ile Arg Ile Leu Tyr Arg Arg Leu
455 460 465ccg ctt att ggg gcg tcg agg ggg gcg tcg aaa agc aaa gtc ttt ggc 1555Pro Leu Ile Gly Ala Ser Arg Gly Ala Ser Lys Ser Lys Val Phe Gly470 475 480 485gct tcg atg gct atg ctt gcc cca ttc ctt gcg tct ggc acc gcg att 1603Ala Ser Met Ala Met Leu Ala Pro Phe Leu Ala Ser Gly Thr Ala Ile
490 495 500ctc atc gcc gtt ttt ggc gat cag act ctg tca acc gtc atg gaa tcc 1651Leu Ile Ala Val Phe Gly Asp Gln Thr Leu Ser Thr Val Met Glu Ser
505 510 515atc agc gtg cgc tcc gcg aag ggc ccg gca ctg acc tgg tac cac gaa 1699Ile Ser Val Arg Ser Ala Lys Gly Pro Ala Leu Thr Trp Tyr His Glu
520 525 530tat gtg cgc tac caa acc gtc atg gaa caa acc gtt gat ggt tcc ttc 1747Tyr Val Arg Tyr Gln Thr Val Met Glu Gln Thr Val Asp Gly Ser Phe
535 540 545acc cgc cgt ttt gct gtg ctg atg ctc atg gcg tgt ctg gct att gtg 1795Thr Arg Arg Phe Ala Val Leu Met Leu Met Ala Cys Leu Ala Ile Val550 555 560 565gtc atc gcg atc ctg cgt tac ggc cgc att cca ggc gct gcg aag gga 1843Val Ile Ala Ile Leu Arg Tyr Gly Arg Ile Pro Gly Ala Ala Lys Gly
570 575 580cca tca ctg cgt ttg atg atg gtc att ttc ggc acc atg ttc ttc atg 1891Pro Ser Leu Arg Leu Met Met Val Ile Phe Gly Thr Met Phe Phe Met
585 590 595atg ttc acc cca acc aag tgg act cac cac ttc ggt gtc tac gca gga 1939Met Phe Thr Pro Thr Lys Trp Thr His His Phe Gly Val Tyr Ala Gly
600 605 610ctt gcc ggc gca ttg gcc gga ctt gct gcc gtg ggg ctg tcc tat gtt 1987Leu Ala Gly Ala Leu Ala Gly Leu Ala Ala Val Gly Leu Ser Tyr Val
615 620 625gcg gtg aaa tca cca cgc atg cgc acc att tcc atc ggt gcg ttc ctc 2035Ala Val Lys Ser Pro Arg Met Arg Thr Ile Ser Ils Gly Ala Phe Leu630 635 640 645ttc ctg ctg gcg ctg gct ctc gca ggc gtg aac gga ttc tgg tac acc 2083Phe Leu Leu Ala Leu Ala Leu Ala Gly Val Asn Gly Phe Trp Tyr Thr
650 655 660tcc agc tac gcc gtg cca tgg tgg gat aaa acc atc cag atc aag ggc 2131Ser Ser Tyr Ala Val Pro Trp Trp Asp Lys Thr Ile Gln Ile Lys Gly
665 670 675atc gaa gca tcc acc gta gtg ctc gtg atc gcc gtg atc gtg ctg atc 2179Ile Glu Ala Ser Thr Val Val Leu Val Ile Ala Val Ile Val Leu Ile
680 685 690atc ggt gtt att caa tcc ttt gtc cac gat gtg aaa acc gcg caa gcc 2227Ile Gly Val Ile Gln Ser Phe Val His Asp Val Lys Thr Ala Gln Ala
695 700 705gaa acc aat cac tcc atg ggc gaa ctc gtg gcg gaa gat gaa gca aag 2275Glu Thr Asn His Ser Met Gly Glu Leu Val Ala Glu Asp Glu Ala Lys710 715 720 725cgc gag cgt gcc tcc agg ttc acc ggc ctt gcg gcc tcc cct atc gca 2323Arg Glu Arg Ala Ser Arg Phe Thr Gly Leu Ala Ala Ser Pro Ile Ala
730 735 740gga gtg tcc gcc ctc gtt gtg ctg att acc tgc gca tcc atg ggc aaa 2371Gly Val Ser Ala Leu Val Val Leu Ile Thr Cys Ala Ser Met Gly Lys
745 750 755ggc ttt gtg gac caa tac ccc gcg tac tcc gtg ggt ctt ggc aac ctc 2419Gly Phe Val Asp Gln Tyr Pro Ala Tyr Ser Val Gly Leu Gly Asn Leu
760 765 770cgc tcc ctg acc ggc aac aca tgt ggc ctt gcc tcc gac gcc atg ctg 2467Arg Ser Leu Thr Gly Asn Thr Cys Gly Leu Ala Ser Asp Ala Met Leu
775 780 785gaa acc aac tcc aac gat tcc ttc ctc act cca gtg aac tcc aca ctt 2515Glu Thr Asn Ser Asn Asp Ser Phe Leu Thr Pro Val Asn Ser Thr Leu790 795 800 805ggc gag tcc ctg gaa tcc gaa gat att cgc ggc ttt agt gct gcc ggc 2563Gly Glu Ser Leu Glu Ser Glu Asp Ile Arg Gly Phe Ser Ala Ala Gly
810 815 820atc cca cca gca agc agc cag gac caa gca gac ctg tct gct gtt ggt 2611Ile Pro Pro Ser Ile Ser Gln Asp Gln Ala Asp Leu Ser Ala Val Gly
825 830 835gcc att gcc aac act gac gac tcc acc gaa acc ggc gga tcc gac gaa 2659Ala Ile Ala Asn Thr Asp Asp Ser Thr Glu Thr Gly Gly Ser Asp Glu
840 845 850tca tcc gga caa tcc acc ggc aac acc ggc ggt gtc cga ggc tcc gaa 2707Ser Ser Gly Gln Ser Thr Gly Asn Thr Gly Gly Val Arg Gly Ser Glu
855 860 865ggc atc aac ggc tcc aac gcc cgc ctg cca ttc aac ctg gac tac acc 2755Gly Ile Asn Gly Ser Asn Ala Arg Leu Pro Phe Asn Leu Asp Tyr Thr870 875 880 885caa gtt cca gtc gtc ggc tcc tgg tcc gct ggc acc caa aac cca gca 2803Gln Val Pro Val Val Gly Ser Trp Ser Ala Gly Thr Gln Asn Pro Ala
890 895 900aac atc acc acc gac tgg tac gaa atc cca gaa gcc acc gaa gaa gca 2851Asn Ile Thr Thr Asp Trp Tyr Glu Ile Pro Glu Ala Thr Glu Glu Ala
905 910 915ccc atc atc gtg gta tct gca gca ggt cgc atc gaa cac tac gac atc 2899Pro Ile Ile Val Val Ser Ala Ala Gly Arg Ile Glu His Tyr Asp Ile
920 925 930aac ggc gtc cgc caa tcc gga caa tcc gtc atg ctc gaa tac ggt cgc 2947Asn Gly Val Arg Gln Ser Gly Gln Ser Val Met Leu Glu Tyr Gly Arg
935 940 945ctt cgc gat aac ggc gac gtt gaa gac ctc ggc gaa gcc atg atg tac 2995Leu Arg Asp Asn Gly Asp Val Glu Asp Leu Gly Glu Ala Met Met Tyr950 955 960 965gac atc ggc ccc gag cca tcc tgg cgc aac ctc cgc tac cca ctt gac 3043Asp Ile Gly Pro Glu Pro Ser Trp Arg Asn Leu Arg Tyr Pro Leu Asp
970 975 980caa ctc cca gaa gaa gcg gac gtc gtg cgc atc gtc gcc acc gac gtc 3091Gln Leu Pro Glu Glu Ala Asp Val Val Arg Ile Val Ala Thr Asp Val
985 990 995aac ctc gac gaa gac caa tgg gta gca ctg acg cca cca cgc gta cct 3139Asn Leu Asp Glu Asp Gln Trp Val Ala Leu Thr Pro Pro Arg Val Pro
1000 1005 1010aac cta gat tct ctg aac aac gtc atc gga tcc gaa acc cca gga ctc 3187Asn Leu Asp Ser Leu Asn Asn Val Ile Gly Ser Glu Thr Pro Gly Leu
1015 1020 1025ctc gac tgg gca gtt ggc ctg caa ttc cca tgc caa cgc acc ttc gac 3235Leu Asp Trp Ala Val Gly Leu Gln Phe Pro Cys Gln Arg Thr Phe Asp1030 1035 1040 1045cac tac gcc gga gtc acc gag att cct gaa tac cga atc tcc cca gac 3283His Tyr Ala Gly Val Thr Glu Ile Pro Glu Tyr Arg Ile Ser Pro Asp
1050 1055 1060cac ggc gga aaa tcc acc ctc tcc cca ttc caa gac tgg gca ggc ggc 3331His Gly Gly Lys Ser Thr Leu Ser Pro Phe Gln Asp Trp Ala Gly Gly
1065 1070 1075gga tcc atg ggc acg gcc gaa gca gta aac aac gcc tac gaa atc ccg 3379Gly Ser Met Gly Thr Ala Glu Ala Val Asn Asn Ala Tyr Glu Ile Pro
1080 1085 1090tcc tac ctc cgc aac gac tgg ggc cgc gac tgg ggt tcc atc gaa cgc 3427Ser Tyr Leu Arg Asn Asp Trp Gly Arg Asp Trp Gly Ser Ile Glu Arg
1095 1100 1105tac tcg ctg cgc acc aac tcc aac ggc gat gca cct aag gtt gca gac 3475Tyr Ser Leu Arg Thr Asn Ser Asn Gly Asp Ala Pro Lys Val Ala Asp1110 1115 1120 1125atc aac ctt gaa acc atc caa cgt tcc gga ctc tgg aat cca ggg cat 3523Ile Asn Leu Glu Thr Ile Gln Arg Ser Gly Leu Trp Asn Pro Gly His
1130 1135 1140atg aag gta gat gaa taagaccttc agtactggaa gtt 3561Met Lys Val Asp Glu
1145<210>476<211>1146<212>PRT<213>谷氨酸棒杆菌<400>476Met Arg Gln Val Gly Gly Asn Gly Gly His Gln Leu Asp Ser Ile Asn1 5 10 15Val Ser Asp Val Val Glu Ser Lys Lys Leu Lys Gly Ser Ala Gln Glu
20 25 30Pro Pro Gln Val Ala Pro Gly Trp Leu Lys Lys Leu Ala Ile Ser Ser
35 40 45Gly Leu Leu Gly Leu Leu Met Phe Val Leu Leu Pro Phe Leu Pro Val
50 55 60Asn Gln Val Gln Ser Ser Leu Ser Trp Pro Gln Asn Gly Glu Leu Ser65 70 75 80Ser Val Asn Ala Pro Leu Ile Ser Tyr Ala Pro Gln Ser Met Asp Ala
85 90 95Ser Ile Pro Val Ser Ala Leu Asp Ser Leu Asn Asp Asn Gln Ser Leu
100 105 110Val Met Gly Thr Leu Pro Leu Asp Ser Thr Asp Ala Thr Asn Arg Gly
115 120 125Leu Phe Val Arg Thr Ile Asp Gly Asn Leu Asp Val Ile Val Arg Gly
130 135 140Glu Val Leu Leu Asp Leu Ser Pro Thr Glu Val Asn Arg Leu Pro Asp145 150 155 160Asp Ala Ile Leu Glu Ile Ser Ser Thr Glu Glu Thr Thr Ser Ala Glu
165 170 175Ile Thr Gly Thr Ala Phe Ser Gly Glu Thr Glu Gly Asp Glu Arg Pro
180 185 190Gln Val Thr Gly Val Tyr Thr Glu Leu Val Asp Asp Pro Ser Thr Ala
195 200 205Ser Ala Leu Ala Ser Ala Gly Leu Asn Val Asp Ile Glu Ile Asn Ser
210 215 220Arg Phe Thr Ser Ser Pro Ser Leu Leu Lys Tyr Ala Ala Ile Phe Ile225 230 235 240Gly Leu Ala Ser Val Leu Val Ser Leu Trp Thr Leu His Arg Met Asp
245 250 255Ile Leu Asp Gly Arg Lys Ala His Arg Phe Leu Pro Ala Asn Trp Tyr
260 265 270Lys Leu Lys Pro Leu Asp Gly Val Val Val Ala Ile Leu Val Phe Trp
275 280 285His Phe Leu Gly Ala Asn Thr Ser Asp Asp Gly Phe Ile Met Thr Met
290 295 300Ala Arg Val Ser Gln Asn Ala Asp Tyr Met Ala Asn Tyr Tyr Arg Trp305 310 315 320Phe Gly Val Pro Glu Ser Pro Phe Gly Ala Pro Tyr Tyr Asp Leu Leu
325 330 335Ala Leu Met Ala Tyr Ile Ser Thr Ser Ser Ile Trp Leu Arg Leu Pro
340 345 350Ala Leu Leu Ala Gly Leu Ile Met Trp Phe Val Ile Thr Arg Glu Val
355 360 365Met Pro Arg Phe Gly Ser Leu Val Asn Gly Arg Arg Val Ala His Trp
370 375 380Ser Ala Ala Met Val Phe Leu Ala Phe Trp Leu Pro Tyr Asn Asn Gly385 390 395 400Thr Arg Pro Glu Pro Ile Ile Ala Met Gly Ala Leu Leu Ala Trp Val
405 410 415Ser Phe Glu Arg Ala Ile Ala Thr Ser Arg Leu Leu Pro Ala Ala Ile
420 425 430Gly Val Ile Ile Ala Thr Ile Ser Leu Ala Ser Gly Pro Thr Gly Leu
435 440 445Met Ala Val Ala Ala Leu Leu Val Ser Leu Ser Ala Leu Ile Arg Ile
450 455 460Leu Tyr Arg Arg Leu Pro Leu Ile Gly Ala Ser Arg Gly Ala Ser Lys465 470 475 480Ser Lys Val Phe Gly Ala Ser Met Ala Met Leu Ala Pro Phe Leu Ala
485 490 495Ser Gly Thr Ala Ile Leu Ile Ala Val Phe Gly Asp Gln Thr Leu Ser
500 505 510Thr Val Met Glu Ser Ile Ser Val Arg Ser Ala Lys Gly Pro Ala Leu
515 520 525Thr Trp Tyr His Glu Tyr Val Arg Tyr Gln Thr Val Met Glu Gln Thr
530 535 540Val Asp Gly Ser Phe Thr Arg Arg Phe Ala Val Leu Met Leu Met Ala545 550 555 560Cys Leu Ala Ile Val Val Ile Ala Ile Leu Arg Tyr Gly Arg Ile Pro
565 570 575Gly Ala Ala Lys Gly Pro Ser Leu Arg Leu Met Met Val Ile Phe Gly
580 585 590Thr Met Phe Phe Met Met Phe Thr Pro Thr Lys Trp Thr His His Phe
595 600 605Gly Val Tyr Ala Gly Leu Ala Gly Ala Leu Ala Gly Leu Ala Ala Val
610 615 620Gly Leu Ser Tyr Val Ala Val Lys Ser Pro Arg Met Arg Thr Ile Ser625 630 635 640Ile Gly Ala Phe Leu Phe Leu Leu Ala Leu Ala Leu Ala Gly Val Asn
645 650 655Gly Phe Trp Tyr Thr Ser Ser Tyr Ala Val Pro Trp Trp Asp Lys Thr
660 665 670Ile Gln Ile Lys Gly Ile Glu Ala Ser Thr Val Val Leu Val Ile Ala
675 680 685Val Ile Val Leu Ile Ile Gly Val Ile Gln Ser Phe Val His Asp Val
690 695 700Lys Thr Ala Gln Ala Glu Thr Asn His Ser Met Gly Glu Leu Val Ala705 710 715 720Glu Asp Glu Ala Lys Arg Glu Arg Ala Ser Arg Phe Thr Gly Leu Ala
725 730 735Ala Ser Pro Ile Ala Gly Val Ser Ala Leu Val Val Leu Ile Thr Cys
740 745 750Ala Ser Met Gly Lys Gly Phe Val Asp Gln Tyr Pro Ala Tyr Ser Val
755 760 765Gly Leu Gly Asn Leu Arg Ser Leu Thr Gly Asn Thr Cys Gly Leu Ala
770 775 780Ser Asp Ala Met Leu Glu Thr Asn Ser Asn Asp Ser Phe Leu Thr Pro785 790 795 800Val Asn Ser Thr Leu Gly Glu Ser Leu Glu Ser Glu Asp Ile Arg Gly
805 810 815Phe Ser Ala Ala Gly Ile Pro Pro Ser Ile Ser Gln Asp Gln Ala Asp
820 825 830Leu Ser Ala Val Gly Ala Ile Ala Asn Thr Asp Asp Ser Thr Glu Thr
835 840 845Gly Gly Ser Asp Glu Ser Ser Gly Gln Ser Thr Gly Asn Thr Gly Gly
850 855 860Val Arg Gly Ser Glu Gly Ile Asn Gly Ser Asn Ala Arg Leu Pro Phe865 870 875 880Asn Leu Asp Tyr Thr Gln Val Pro Val Val Gly Ser Trp Ser Ala Gly
885 890 895Thr Gln Asn Pro Ala Asn Ile Thr Thr Asp Trp Tyr Glu Ile Pro Glu
900 905 910Ala Thr Glu Glu Ala Pro Ile Ile Val Val Ser Ala Ala Gly Arg Ile
915 920 925Glu His Tyr Asp Ile Asn Gly Val Arg Gln Ser Gly Gln Ser Val Met
930 935 940Leu Glu Tyr Gly Arg Leu Arg Asp Asn Gly Asp Val Glu Asp Leu Gly945 950 955 960Glu Ala Met Met Tyr Asp Ile Gly Pro Glu Pro Ser Trp Arg Asn Leu
965 970 975Arg Tyr Pro Leu Asp Gln Leu Pro Glu Glu Ala Asp Val Val Arg Ile
980 985 990Val Ala Thr Asp Val Asn Leu Asp Glu Asp Gln Trp Val Ala Leu Thr
995 1000 1005Pro Pro Arg Val Pro Asn Leu Asp Ser Leu Asn Asn Val Ile Gly Ser
1010 1015 1020Glu Thr Pro Gly Leu Leu Asp Trp Ala Val Gly Leu Gln Phe Pro Cys1025 1030 1035 1040Gln Arg Thr Phe Asp His Tyr Ala Gly Val Thr Glu Ile Pro Glu Tyr
1045 1050 1055Arg Ile Ser Pro Asp His Gly Gly Lys Ser Thr Leu Ser Pro Phe Gln
1060 1065 1070Asp Trp Ala Gly Gly Gly Ser Met Gly Thr Ala Glu Ala Val Asn Asn
1075 1080 1085Ala Tyr Glu Ile Pro Ser Tyr Leu Arg Asn Asp Trp Gly Arg Asp Trp
1090 1095 1100Gly Ser Ile Glu Arg Tyr Ser Leu Arg Thr Asn Ser Asn Gly Asp Ala1105 1110 1115 1120Pro Lys Val Ala Asp Ile Asn Leu Glu Thr Ile Gln Arg Ser Gly Leu
1125 1130 1135Trp Asn Pro Gly His Met Lys Val Asp Glu
1140 1145<210>477<211>1509<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1486)<223>RXN01175<400>477tcgcgtctct tagtcgatct gctcccattc cataggttgg gagaaaactt aactgttatt 60ccattcctta aaacaccgat atcgtgctat gaataggggt gtg agt tgg aca gtt 115
Val Ser Trp Thr Val
1 5gat atc cct aaa gaa gtt ctc cct gat ttg cca cca ttg cca gaa ggc 163Asp Ile Pro Lys Glu Val Leu Pro Asp Leu Pro Pro Leu Pro Glu Gly
10 15 20atg cag cag cag ttc gag gac acc att tcc cgt gac gct aag cag caa 211Met Gln Gln Gln Phe Glu Asp Thr Ile Ser Arg Asp Ala Lys Gln Gln
25 30 35cct acg tgg gat cgt gca cag gca gaa aac gtg cgc aag atc ctt gag 259Pro Thr Trp Asp Arg Ala Gln Ala Glu Asn Val Arg Lys Ile Leu Glu
40 45 50tcg gtt cct cca atc gtt gtt gcc cct gag gta ctt gag ctg aag cag 307Ser Val Pro Pro Ile Val Val Ala Pro Glu Val Leu Glu Leu Lys Gln
55 60 65aag ctt gct gat gtt gcc aac ggt aag gcc ttc ctc ttg cag ggt ggt 355Lys Leu Ala Asp Val Ala Asn Gly Lys Ala Phe Leu Leu Gln Gly Gly70 75 80 85gac tgt gcg gaa act ttc gag tca aac act gag ccg cac att cgc gcc 403Asp Cys Ala Glu Thr Phe Glu Ser Asn Thr Glu Pro His Ile Arg Ala
90 95 100aac gta aag act ctg ctg cag atg gca gtt gtt ttg acc tac ggt gca 451Asn Val Lys Thr Leu Leu Gln Met Ala Val Val Leu Thr Tyr Gly Ala
105 110 115tcc act cct gtg atc aag atg gct cgt att gct ggt cag tac gca aag 499Ser Thr Pro Val Ile Lys Met Ala Arg Ile Ala Gly Gln Tyr Ala Lys
120 125 130cct cgc tct tct gat ctg gat gga aat ggt ctg cca aac tac cgt ggc 547Pro Arg Ser Ser Asp Leu Asp Gly Asn Gly Leu Pro Asn Tyr Arg Gly
135 140 145gat atc gtc aac ggt gtg gag gca acc cca gag gct cgt cgc cac gat 595Asp Ile Val Asn Gly Val Glu Ala Thr Pro Glu Ala Arg Arg His Asp150 155 160 165cct gcc cgc atg atc cgt gct tac gct aac gct tct gct gcg atg aac 643Pro Ala Arg Met Ile Arg Ala Tyr Ala Asn Ala Ser Ala Ala Met Asn
170 175 180ttg gtg cgc gcg ctc acc agc tct ggc acc gct gat ctt tac cgt ctc 691Leu Val Arg Ala Leu Thr Ser Ser Gly Thr Ala Asp Leu Tyr Arg Leu
185 190 195agc gag tgg aac cgc gag ttc gtt gcg aac tcc cca gct ggt gca cgc 739Ser Glu Trp Asn Arg Glu Phe Val Ala Asn Ser Pro Ala Gly Ala Arg
200 205 210tac gag gct ctt gct cgt gag atc gac tcc ggt ctg cgc ttc atg gaa 787Tyr Glu Ala Leu Ala Arg Glu Ile Asp Ser Gly Leu Arg Phe Met Glu
215 220 225gca tgt ggc gtg tcc gat gag tcc ctg cgt gct gca gat atc tac tgc 835Ala Cys Gly Val Ser Asp Glu Ser Leu Arg Ala Ala Asp Ile Tyr Cys230 235 240 245tcc cac gag gct ttg ctg gtg gat tac gag cgt tcc atg ctg cgt ctt 883Ser His Glu Ala Leu Leu Val Asp Tyr Glu Arg Ser Met Leu Arg Leu
250 255 260gca acc gat gag gaa ggc aac gag gaa ctt tac gat ctt tca gct cac 931Ala Thr Asp Glu Glu Gly Asn Glu Glu Leu Tyr Asp Leu Ser Ala His
265 270 275cag ctg tgg atc ggc gag cgc acc cgt ggc atg gat gat ttc cat gtg 979Gln Leu Trp Ile Gly Glu Arg Thr Arg Gly Met Asp Asp Phe His Val
280 285 290aac ttc gca tcc atg atc tct aac cca atc ggc atc aag att ggt cct 1027Asn Phe Ala Ser Met Ile Ser Asn Pro Ile Gly Ile Lys Ile Gly Pro
295 300 305ggt atc acc cct gaa gag gct gtt gca tac gct gac aag ctc gat ccg 1075Gly Ile Thr Pro Glu Glu Ala Val Ala Tyr Ala Asp Lys Leu Asp Pro310 315 320 325aac ttc gag cct ggc cgt ttg acc atc gtt gct cgc atg ggc cac gac 1123Asn Phe Glu Pro Gly Arg Leu Thr Ile Val Ala Arg Met Gly His Asp
330 335 340aag gtt cgc tcc gta ctt cct ggt gtt atc cag gct gtt gag gca tcc 1171Lys Val Arg Ser Val Leu Pro Gly Val Ile Gln Ala Val Glu Ala Ser
345 350 355gga cac aag gtt att tgg cag tcc gat ccg atg cac ggc aac act ttc 1219Gly His Lys Val Ile Trp Gln Ser Asp Pro Met His Gly Asn Thr Phe
360 365 370acc gca tcc aat ggc tac aag acc cgt cac ttc gac aag gtt atc gat 1267Thr Ala Ser Asn Gly Tyr Lys Thr Arg His Phe Asp Lys Val Ile Asp
375 380 385gag gtc cag ggc ttc ttc gag gtc cac cgc gca ttg ggc acc cac cca 1315Glu Val Gln Gly Phe Phe Glu Val His Arg Ala Leu Gly Thr His Pro390 395 400 405ggc gga atc cac att gag ttc act ggt gaa gat gtc acc gag tgc ctc 1363Gly Gly Ile His Ile Glu Phe Thr Gly Glu Asp Val Thr Glu Cys Leu
410 415 420ggt ggc gct gaa gac atc acc gat gtt gat ctg cca ggc cgc tac gag 1411Gly Gly Ala Glu Asp Ile Thr Asp Val Asp Leu Pro Gly Arg Tyr Glu
425 430 435tcc gca tgc gat cct cgc ctg aac act cag cag tct ttg gag ttg gct 1459Ser Ala Cys Asp Pro Arg Leu Asn Thr Gln Gln Ser Leu Glu Leu Ala
440 445 450ttc ctc gtt gca gaa atg ctg cgt aac taagctttta gaccaactct 1506Phe Leu Val Ala Glu Met Leu Arg Asn
455 460aaa 1509<210>478<211>462<212>PRT<213>谷氨酸棒杆菌<400>478Val Ser Trp Thr Val Asp Ile Pro Lys Glu Val Leu Pro Asp Leu Pro1 5 10 15Pro Leu Pro Glu Gly Met Gln Gln Gln Phe Glu Asp Thr Ile Ser Arg
20 25 30Asp Ala Lys Gln Gln Pro Thr Trp Asp Arg Ala Gln Ala Glu Asn Val
35 40 45Arg Lys Ile Leu Glu Ser Val Pro Pro Ile Val Val Ala Pro Glu Val
50 55 60Leu Glu Leu Lys Gln Lys Leu Ala Asp Val Ala Asn Gly Lys Ala Phe65 70 75 80Leu Leu Gln Gly Gly Asp Cys Ala Glu Thr Phe Glu Ser Asn Thr Glu
85 90 95Pro His Ile Arg Ala Asn Val Lys Thr Leu Leu Gln Met Ala Val Val
100 105 110Leu Thr Tyr Gly Ala Ser Thr Pro Val Ile Lys Met Ala Arg Ile Ala
115 120 125Gly Gln Tyr Ala Lys Pro Arg Ser Ser Asp Leu Asp Gly Asn Gly Leu
130 135 140Pro Asn Tyr Arg Gly Asp Ile Val Asn Gly Val Glu Ala Thr Pro Glu145 150 155 160Ala Arg Arg His Asp Pro Ala Arg Met Ile Arg Ala Tyr Ala Asn Ala
165 170 175Ser Ala Ala Met Asn Leu Val Arg Ala Leu Thr Ser Ser Gly Thr Ala
180 185 190Asp Leu Tyr Arg Leu Ser Glu Trp Asn Arg Glu Phe Val Ala Asn Ser
195 200 205Pro Ala Gly Ala Arg Tyr Glu Ala Leu Ala Arg Glu Ile Asp Ser Gly
210 215 220Leu Arg Phe Met Glu Ala Cys Gly Val Ser Asp Glu Ser Leu Arg Ala225 230 235 240Ala Asp Ile Tyr Cys Ser His Glu Ala Leu Leu Val Asp Tyr Glu Arg
245 250 255Ser Met Leu Arg Leu Ala Thr Asp Glu Glu Gly Asn Glu Glu Leu Tyr
260 265 270Asp Leu Ser Ala His Gln Leu Trp Ile Gly Glu Arg Thr Arg Gly Met
275 280 285Asp Asp Phe His Val Asn Phe Ala Ser Met Ile Ser Asn Pro Ile Gly
290 295 300Ile Lys Ile Gly Pro Gly Ile Thr Pro Glu Glu Ala Val Ala Tyr Ala305 310 315 320Asp Lys Leu Asp Pro Asn Phe Glu Pro Gly Arg Leu Thr Ile Val Ala
325 330 335Arg Met Gly His Asp Lys Val Arg Ser Val Leu Pro Gly Val Ile Gln
340 345 350Ala Val Glu Ala Ser Gly His Lys Val Ile Trp Gln Set Asp Pro Met
355 360 365His Gly Asn Thr Phe Thr Ala Ser Asn Gly Tyr Lys Thr Arg His Phe
370 375 380Asp Lys Val Ile Asp Glu Val Gln Gly Phe Phe Glu Val His Arg Ala385 390 395 400Leu Gly Thr His Pro Gly Gly Ile His Ile Glu Phe Thr Gly Glu Asp
405 410 415Val Thr Glu Cys Leu Gly Gly Ala Glu Asp Ile Thr Asp Val Asp Leu
420 425 430Pro Gly Arg Tyr Glu Ser Ala Cys Asp Pro Arg Leu Asn Thr Gln Gln
435 440 445Ser Leu Glu Leu Ala Phe Leu Val Ala Glu Met Leu Arg Asn
450 455 460<210>479<211>984<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(961)<223>RXN0l376<400>479tcctcatcgg ttatcgggag tggcggatac ttcttgcaaa cttaaaccac tatgctttcg 60cttcgtgagt actttgaaat cccccatcgc tgtgatcaca gtg acc tat tca cca 115
Val Thr Tyr Ser Pro
1 5ggt aaa tac ttg gcg tcg ttc ctg gat tct ttg cct ggt gcg act tca 163Gly Lys Tyr Leu Ala Ser Phe Leu Asp Ser Leu Pro Gly Ala Thr Ser
10 15 20cga gac acc cac gtt gtg atg gca gac aat ggt tct gtg gac ggt gtt 211Arg Asp Thr His Val Val Met Ala Asp Asn Gly Ser Val Asp Gly Val
25 30 35cct gag cag gca gca gcc tca cgc agc aac gtg gag ttc ctc tca act 259Pro Glu Gln Ala Ala Ala Ser Arg Ser Asn Val Glu Phe Leu Ser Thr
40 45 50ggc ggc aac tta ggc tac gga acg gct att aat att gcc gcc cga tcg 307Gly Gly Asn Leu Gly Tyr Gly Thr Ala Ile Asn Ile Ala Ala Arg Ser
55 60 65ttg cgt gcg cgc cgg gag gca gga gag atc gat ggg gag ttc ttc ctc 355Leu Arg Ala Arg Arg Glu Ala Gly Glu Ile Asp Gly Glu Phe Phe Leu70 75 80 85gtc tca aac cct gat gtt gtt ttt gac gaa gac tct att gat caa ttg 403Val Ser Asn Pro Asp Val Val Phe Asp Glu Asp Ser Ile Asp Gln Leu
90 95 100ctt gaa tgt gcg aaa cgt cac cct gaa gca gga gcg gtt ggc ccg ttg 451Leu Glu Cys Ala Lys Arg His Pro Glu Ala Gly Ala Val Gly Pro Leu
105 110 115atc cgt gag gcg gac ggt tcg gcg tat ccg tcg gct cgg gcg gta ccc 499Ile Arg Glu Ala Asp Gly Ser Ala Tyr Pro Ser Ala Arg Ala Val Pro
120 125 130act ttg gcg aat ggc att ggt cac gct ttg ttg ggt gct gtg tgg aaa 547Thr Leu Ala Asn Gly Ile Gly His Ala Leu Leu Gly Ala Val Trp Lys
135 140 145tcc aat ccg tgg tcg gcg gct tac cgt gac gat gaa gat atg gac act 595Ser Asn Pro Trp Ser Ala Ala Tyr Arg Asp Asp Glu Asp Met Asp Thr150 155 160 165gag cgc act gct ggc tgg ctg tcg gga tcg tgc ctg tta tta agg tgg 643Glu Arg Thr Ala Gly Trp Leu Ser Gly Ser Cys Leu Leu Leu Arg Trp
170 175 180gat gcg ttt gat cga gtt ggt ggt ttt gat gag cgc tac ttc atg tac 691Asp Ala Phe Asp Arg Val Gly Gly Phe Asp Glu Arg Tyr Phe Met Tyr
185 190 195atg gaa gac gtt gac ctg gga gat cgg ctg gtt cgc gcc ggt ttc acc 739Met Glu Asp Val Asp Leu Gly Asp Arg Leu Val Arg Ala Gly Phe Thr
200 205 210aac gtc ttt tgc cca agt gcg cag atc atc cac gcg aaa ggt cat gtt 787Asn Val Phe Cys Pro Ser Ala Gln Ile Ile His Ala Lys Gly His Val
215 220 225gcg ggt aaa aac cca gag aac atg ttg ccc gca cac cac gag agc gcg 835Ala Gly Lys Asn Pro Glu Asn Met Leu Pro Ala His His Glu Ser Ala230 235 240 245tat cgc ttc cag gct gat cgc ctc gcg aag ccg tgg caa gcc cca att 883Tyr Arg Phe Gln Ala Asp Arg Leu Ala Lys Pro Trp Gln Ala Pro Ile
250 255 260cgg ttg gct ctg cga att ggt ttg aaa tta cga gcc gga gtc gcg gtt 931Arg Leu Ala Leu Arg Ile Gly Leu Lys Leu Arg Ala Gly Val Ala Val
265 270 275ggt gtc tct aag atg aga acg aaa gcc tct tagaccgtcg acgaccaccg 981Gly Val Ser Lys Met Arg Thr Lys Ala Ser
280 285gac 984<210>480<211>287<212>PRT<213>谷氨酸棒杆菌<400>480Val Thr Tyr Ser Pro Gly Lys Tyr Leu Ala Ser Phe Leu Asp Ser Leu1 5 10 15Pro Gly Ala Thr Ser Arg Asp Thr His Val Val Met Ala Asp Asn Gly
20 25 30Ser Val Asp Gly Val Pro Glu Gln Ala Ala Ala Ser Arg Ser Asn Val
35 40 45Glu Phe Leu Ser Thr Gly Gly Asn Leu Gly Tyr Gly Thr Ala Ile Asn
50 55 60Ile Ala Ala Arg Ser Leu Arg Ala Arg Arg Glu Ala Gly Glu Ile Asp65 70 75 80Gly Glu Phe Phe Leu Val Ser Asn Pro Asp Val Val Phe Asp Glu Asp
85 90 95Ser Ile Asp Gln Leu Leu Glu Cys Ala Lys Arg His Pro Glu Ala Gly
100 105 110Ala Val Gly Pro Leu Ile Arg Glu Ala Asp Gly Ser Ala Tyr Pro Ser
115 120 125Ala Arg Ala Val Pro Thr Leu Ala Asn Gly Ile Gly His Ala Leu Leu
130 135 140Gly Ala Val Trp Lys Ser Asn Pro Trp Ser Ala Ala Tyr Arg Asp Asp145 150 155 160Glu Asp Met Asp Thr Glu Arg Thr Ala Gly Trp Leu Ser Gly Ser Cys
165 170 175Leu Leu Leu Arg Trp Asp Ala Phe Asp Arg Val Gly Gly Phe Asp Glu
180 185 190Arg Tyr Phe Met Tyr Met Glu Asp Val Asp Leu Gly Asp Arg Leu Val
195 200 205Arg Ala Gly Phe Thr Asn Val Phe Cys Pro Ser Ala Gln Ile Ile His
210 215 220Ala Lys Gly His Val Ala Gly Lys Asn Pro Glu Asn Met Leu Pro Ala225 230 235 240His His Glu Ser Ala Tyr Arg Phe Gln Ala Asp Arg Leu Ala Lys Pro
245 250 255Trp Gln Ala Pro Ile Arg Leu Ala Leu Arg Ile Gly Leu Lys Leu Arg
260 265 270Ala Gly Val Ala Val Gly Val Ser Lys Met Arg Thr Lys Ala Ser
275 280 285<210>481<211>1002<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(979)<223>RXN01631<400>481gccagttgag gatgctctca cagtcgctcg cattgtcagt gcatgctacg aatccgacaa 60caaccagggc atttccgtaa acatctaaga ggagcactcc atg aaa cca caa ctt 115
Met Lys Pro Gln Leu
1 5att gca tct tgc tgg acc agc gcg gga gac gcc gca ccc gat cgt gac 163Ile Ala Ser Cys Trp Thr Ser Ala Gly Asp Ala Ala Pro Asp Arg Asp
10 15 20gat ctc agc agc cca gta gca atc gat gag cgc atc gct cta gtc gct 211Asp Leu Ser Ser Pro Val Ala Ile Asp Glu Arg Ile Ala Leu Val Ala
25 30 35gaa acc ggt tgg gca ggc att ggg ctt gtt cac gcc gat ctc atc aaa 259Glu Thr Gly Trp Ala Gly Ile Gly Leu Val His Ala Asp Leu Ile Lys
40 45 50gca cgc gac acc att ggc tac gag gaa ttg cgc cga cgc atc cac gct 307Ala Arg Asp Thr Ile Gly Tyr Glu Glu Leu Arg Arg Arg Ile His Ala
55 60 65gca gga att gaa atc att gag gtg gag ttc ctc aat ggt tgg tgg gcg 355Ala Gly Ile Glu Ile Ile Glu Val Glu Phe Leu Asn Gly Trp Trp Ala70 75 80 85act ggt gcg gaa cgc caa gag tcc gat gcc gtt cgt gcg gat ctg ttt 403Thr Gly Ala Glu Arg Gln Glu Ser Asp Ala Val Arg Ala Asp Leu Phe
90 95 100gct gcg gcg caa gct ctt ggt tcc cca cac att aag gtc gga gca gga 451Ala Ala Ala Gln Ala Leu Gly Ser Pro His Ile Lys Val Gly Ala Gly
105 110 115gag ggc acc aat ggt gtg gtt ccc att gct cac atg gcc agt gcg ttt 499Glu Gly Thr Asn Gly Val Val Pro Ile Ala His Met Ala Ser Ala Phe
120 125 130act gat ctc gct gcg gaa gct gaa gct cat ggc gtc aag ctc gcg ttg 547Thr Asp Leu Ala Ala Glu Ala Glu Ala His Gly Val Lys Leu Ala Leu
135 140 145gaa gca act ccg ttt tct cac ctg aag acc atc tac gac gcg ctg gaa 595Glu Ala Thr Pro Phe Ser His Leu Lys Thr Ile Tyr Asp Ala Leu Glu150 155 160 165gtt gtc agc cat tcc gat agc cca tcg gct gga ctc atg gtt gat atc 643Val Val Ser His Ser Asp Ser Pro Ser Ala Gly Leu Met Val Asp Ile
170 175 180tgg cac acc gcg aaa atc gga atc ccc aac gat gaa ctg tgg cgc aac 691Trp His Thr Ala Lys Ile Gly Ile Pro Asn Asp Glu Leu Trp Arg Asn
185 190 195att cca ctg tcc aag gtc aac gca gtg gag gtt gat gat ggt ttc att 739Ile Pro Leu Ser Lys Val Asn Ala Val Glu Val Asp Asp Gly Phe Ile
200 205 210gac acc cca att gat ctt ttc gat gac tcc acc aac cgt cgc gcg tac 787Asp Thr Pro Ile Asp Leu Phe Asp Asp Ser Thr Asn Arg Arg Ala Tyr
215 220 225tgc ggt gaa ggc gaa ttt gat ccc gca agc ttc atc cgt ggc gcc atc 835Cys Gly Glu Gly Glu Phe Asp Pro Ala Ser Phe Ile Arg Gly Ala Ile230 235 240 245gac gcc ggt tgg acg ggc gca tat ggt gtg gaa att att tcc gca gag 883Asp Ala Gly Trp Thr Gly Ala Tyr Gly Val Glu Ile Ile Ser Ala Glu
250 255 260cac cga agc ctc ccg gtg aaa gaa ggg ctg caa cgt gct ttc gac acc 931His Arg Ser Leu Pro Val Lys Glu Gly Leu Gln Arg Ala Phe Asp Thr
265 270 275acc atc gca gcg ttt gaa caa gct gct cgt ctc gcc ccc tcc act aac 979Thr Ile Ala Ala Phe Glu Gln Ala Ala Arg Leu Ala Pro Ser Thr Asn
280 285 290tgatctttga aaggctgaaa aaa 1002<210>482<211>293<212>PRT<213>谷氨酸棒杆菌<400>482Met Lys Pro Gln Leu Ile Ala Ser Cys Trp Thr Ser Ala Gly Asp Ala1 5 10 15Ala Pro Asp Arg Asp Asp Leu Ser Ser Pro Val Ala Ile Asp Glu Arg
20 25 30Ile Ala Leu Val Ala Glu Thr Gly Trp Ala Gly Ile Gly Leu Val His
35 40 45Ala Asp Leu Ile Lys Ala Arg Asp Thr Ile Gly Tyr Glu Glu Leu Arg
50 55 60Arg Arg Ile His Ala Ala Gly Ile Glu Ile Ile Glu Val Glu Phe Leu65 70 75 80Asn Gly Trp Trp Ala Thr Gly Ala Glu Arg Gln Glu Ser Asp Ala Val
85 90 95Arg Ala Asp Leu Phe Ala Ala Ala Gln Ala Leu Gly Ser Pro His Ile
100 105 110Lys Val Gly Ala Gly Glu Gly Thr Asn Gly Val Val Pro Ile Ala His
115 120 125Met Ala Ser Ala Phe Thr Asp Leu Ala Ala Glu Ala Glu Ala His Gly
130 135 140Val Lys Leu Ala Leu Glu Ala Thr Pro Phe Ser His Leu Lys Thr Ile145 150 155 160Tyr Asp Ala Leu Glu Val Val Ser His Ser Asp Ser Pro Ser Ala Gly
165 170 175Leu Met Val Asp Ile Trp His Thr Ala Lys Ile Gly Ile Pro Asn Asp
180 185 190Glu Leu Trp Arg Asn Ile Pro Leu Ser Lys Val Asn Ala Val Glu Val
195 200 205Asp Asp Gly Phe Ile Asp Thr Pro Ile Asp Leu Phe Asp Asp Ser Thr
210 215 220Asn Arg Arg Ala Tyr Cys Gly Glu Gly Glu Phe Asp Pro Ala Ser Phe225 230 235 240Ile Arg Gly Ala Ile Asp Ala Gly Trp Thr Gly Ala Tyr Gly Val Glu
245 250 255Ile Ile Ser Ala Glu His Arg Ser Leu Pro Val Lys Glu Gly Leu Gln
260 265 270Arg Ala Phe Asp Thr Thr Ile Ala Ala Phe Glu Gln Ala Ala Arg Leu
275 280 285Ala Pro Ser Thr Asn
290<210>483<211>990<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(967)<223>RXN01593<400>483atatgacagc cttcttcttg atctagatgg aaccgtctac gagggcggcc gagccattga 60gcacgtagtt tctgcgctct ctggcgccgg cctacccgtc atg tat gtc acc aat 115
Met Tyr Val Thr Asn
1 5aat gcc tcc cgt gct ccg gag gtg gtg gct gcg caa ctc cgt gag att 163Asn Ala Ser Arg Ala Pro Glu Val Val Ala Ala Gln Leu Arg Glu Ile
10 15 20ggc ctt gcc gac acc act gcg gac aat gtg atg aca tct gct caa gct 211Gly Leu Ala Asp Thr Thr Ala Asp Asn Val Met Thr Ser Ala Gln Ala
25 30 35gcc tgc aag atg gcg gcg gag aag att ccc gct gga tcc aag gtg tat 259Ala Cys Lys Met Ala Ala Glu Lys Ile Pro Ala Gly Ser Lys Val Tyr
40 45 50gtt ttg ggt tca gaa tcc ttc cgc gag cta gct act gaa gct ggt ttt 307Val Leu Gly Ser Glu Ser Phe Arg Glu Leu Ala Thr Glu Ala Gly Phe
55 60 65gtg gtg gtt gat tcg gct gat gat aaa cct gtg gct gtg ctt cac ggc 355Val Val Val Asp Ser Ala Asp Asp Lys Pro Val Ala Val Leu His Gly70 75 80 85cac aac cct gag acc ggt tgg gct cag ttg agc gag gct gcg ctg tca 403His Asn Pro Glu Thr Gly Trp Ala Gln Leu Ser Glu Ala Ala Leu Ser
90 95 100att aat gct ggc gcg cag tat ttt gca tca aat ttg gat tcc acc ctt 451Ile Asn Ala Gly Ala Gln Tyr Phe Ala Ser Asn Leu Asp Ser Thr Leu
105 110 115ccc atg gaa cgc ggt cgt cac att ggc aac ggt tcc atg gtg gct gcc 499Pro Met Glu Arg Gly Arg His Ile Gly Asn Gly Ser Met Val Ala Ala
120 125 130gtg gtc aac gcg act ggc gta aag cct ctt tcc gca ggt aag cca ggc 547Val Val Asn Ala Thr Gly Val Lys Pro Leu Ser Ala Gly Lys Pro Gly
135 140 145ccc ggc atg ttc tat gcg ggg gct gaa act ctt aat tct tca aag cct 595Pro Ala Met Phe Tyr Ala Gly Ala Glu Thr Leu Asn Ser Ser Lys Pro150 155 160 165ttg gct gtc ggc gat cgt ctc gat acc gat atc gcc ggc gga aac gct 643Leu Ala Val Gly Asp Arg Leu Asp Thr Asp Ile Ala Gly Gly Asn Ala
170 175 180gca ggc atg gac aca ttc cag gtc ctg acc ggc gtc agc ggc tac tac 691Ala Gly Met Asp Thr Phe Gln Val Leu Thr Gly Val Ser Gly Tyr Tyr
185 190 195gat ttg gtg cgc gcc att ccc aga gca gcg ccc cac cta tat cgc cac 739Asp Leu Val Arg Ala Ile Pro Arg Ala Ala Pro His Leu Tyr Arg His
200 205 210ctc gat gca gga tct cta cag cga tcc ggg cga gct caa gcc agg tgc 787Leu Asp Ala Gly Ser Leu Gln Arg Ser Gly Arg Ala Gln Ala Arg Cys
215 220 225cca ggg cgg ttt ttc agc gct tat cga cgg cga cac cct ggt cat ttc 835Pro Gly Arg Phe Phe Ser Ala Tyr Arg Arg Arg His Pro Gly His Phe230 235 240 245cgg cgg cga tgc cgg cgc aac tcc ggt tgc agc act ccg cac tgc gtt 883Arg Arg Arg Cys Arg Arg Asn Ser Gly Cys Ser Thr Pro His Cys Val
250 255 260gga tgt ggc ctg ggc ggc cac aga gca gtc acc gag gta cgc gct gat 931Gly Cys Gly Leu Gly Gly His Arg Ala Val Thr Glu Val Arg Ala Asp
265 270 275tca gag gta gct gct act gca ttg cag agc tgg tgg taaacggtga 977Ser Glu Val Ala Ala Thr Ala Leu Gln Ser Trp Trp
280 285attcaccaaa gcc 990<210>484<211>289<212>PRT<213>谷氨酸棒杆菌<400>484Met Tyr Val Thr Asn Asn Ala Ser Arg Ala Pro Glu Val Val Ala Ala1 5 10 15Gln Leu Arg Glu Ile Gly Leu Ala Asp Thr Thr Ala Asp Asn Val Met
20 25 30Thr Ser Ala Gln Ala Ala Cys Lys Met Ala Ala Glu Lys Ile Pro Ala
35 40 45Gly Ser Lys Val Tyr Val Leu Gly Ser Glu Ser Phe Arg Glu Leu Ala
50 55 60Thr Glu Ala Gly Phe Val Val Val Asp Ser Ala Asp Asp Lys Pro Val65 70 75 80Ala Val Leu His Gly His Asn Pro Glu Thr Gly Trp Ala Gln Leu Ser
85 90 95Glu Ala Ala Leu Ser Ile Asn Ala Gly Ala Gln Tyr Phe Ala Ser Asn
100 105 110Leu Asp Ser Thr Leu Pro Met Glu Arg Gly Arg His Ile Gly Asn Gly
115 120 125Ser Met Val Ala Ala Val Val Asn Ala Thr Gly Val Lys Pro Leu Ser
130 135 140Ala Gly Lys Pro Gly Pro Ala Met Phe Tyr Ala Gly Ala Glu Thr Leu145 150 155 160Asn Ser Ser Lys Pro Leu Ala Val Gly Asp Arg Leu Asp Thr Asp Ile
165 170 175Ala Gly Gly Asn Ala Ala Gly Met Asp Thr Phe Gln Val Leu Thr Gly
180 185 190Val Ser Gly Tyr Tyr Asp Leu Val Arg Ala Ile Pro Arg Ala Ala Pro
195 200 205His Leu Tyr Arg His Leu Asp Ala Gly Ser Leu Gln Arg Ser Gly Arg
210 215 220Ala Gln Ala Arg Cys Pro Gly Arg Phe Phe Ser Ala Tyr Arg Arg Arg225 230 235 240His Pro Gly His Phe Arg Arg Arg Cys Arg Arg Asn Ser Gly Cys Ser
245 250 255Thr Pro His Cys Val Gly Cys Gly Leu Gly Gly His Arg Ala Val Thr
260 265 270Glu Val Arg Ala Asp Ser Glu Val Ala Ala Thr Ala Leu Gln Ser Trp
275 280 285Trp<210>485<211>1173<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1150)<223>RXN00337<400>485ggacgcttat tggtgagcat tcggattacg ccggtggtgt ggtgctggcg gctaatgcga 60attgccggac tgcggctgcc gtcaataaag aaccgcgacg atg ttg tta acg tat 115
Met Leu Leu Thr Tyr
1 5gcg ttt gtg gat gtg gag gga ggc gtc gaa aag cat tct tta agc act 163Ala Phe Val Asp Val Glu Gly Gly Val Glu Lys His Ser Leu Ser Thr
10 15 20gcg gac att gca gct cgc gca cac gcc cat atg aaa tcc cat gat gtt 211Ala Asp Ile Ala Ala Arg Ala His Ala His Met Lys Ser His Asp Val
25 30 35ttg ggg cgg cag act acg ccg cct cag ccg gag ggc ggc gtt gct gcc 259Leu Gly Arg Gln Thr Thr Pro Pro Gln Pro Glu Gly Gly Val Ala Ala
40 45 50cgg ttg ggc ggg att gcg tgg aca atg atc cat aag caa atg ctt tcg 307Arg Leu Gly Gly Ile Ala Trp Thr Met Ile His Lys Gln Met Leu Ser
55 60 65cgt gac aca aaa ggc ctg gat atc acc gtg ttg agc acc att cct gag 355Arg Asp Thr Lys Gly Leu Asp Ile Thr Val Leu Ser Thr Ile Pro Glu70 75 80 85ggg gtg ggg ctg ggt gaa aat tcc gcc atg gat gtg gcg ctc gca ttg 403Gly Val Gly Leu Gly Glu Asn Ser Ala Met Asp Val Ala Leu Ala Leu
90 95 100gcg ctg tat cgg gaa aat att gag gaa gcc ccc acg aag gcg cgc att 451Ala Leu Tyr Arg Glu Asn Ile Glu Glu Ala Pro Thr Lys Ala Arg Ile
105 110 115gcg gag att tgt tcg cag tcc gca ttc atg ttc agt gag act tca gtg 499Ala Glu Ile Cys Ser Gln Ser Ala Phe Met Phe Ser Glu Thr Ser Val
120 125 130ttg cgt gcg cgg cac acc gtg gcg ttg cgg ggt gaa act gga cag att 547Leu Arg Ala Arg His Thr Val Ala Leu Arg Gly Glu Thr Gly Gln Ile
135 140 145tcg gtg gtt gat tac gcc gat ggt tcg gtc act cag gcg cca cat ccg 595Ser Val Val Asp Tyr Ala Asp Gly Ser Val Thr Gln Ala Pro His Pro150 155 160 165gtg agt cgt tcc gct ggt ttg tcg gca ttt gtt gtt gct gcg caa act 643Val Ser Arg Ser Ala Gly Leu Ser Ala Phe Val Val Ala Ala Gln Thr
170 175 180gaa act gat ccg agc att tac cgc gag atc tat gct cga cat gcg ttt 691Glu Thr Asp Pro Ser Ile Tyr Arg Glu Ile Tyr Ala Arg His Ala Phe
185 190 195atc gat gaa gct gcg cgc gct ttc agt gtg gaa tct ttg cgg ttg ctt 739Ile Asp Glu Ala Ala Arg Ala Phe Ser Val Glu Ser Leu Arg Leu Leu
200 205 210ccc gac gct tcc act cgt gtt gtg gat tgg ttg cag gcc gtg att gag 787Pro Asp Ala Ser Thr Arg Val Val Asp Trp Leu Gln Ala Val Ile Glu
215 220 225gtg act ggt cga gag gat ctg ccc tcg att gaa caa gcc cag cgc tgg 835Val Thr Gly Arg Glu Asp Leu Pro Ser Ile Glu Gln Ala Gln Arg Trp230 235 240 245ttg aat ctg tgg gaa aac gaa acc cgg cgc gct cag agg aca gcc aat 883Leu Asn Leu Trp Glu Asn Glu Thr Arg Arg Ala Gln Arg Thr Ala Asn
250 255 260gcc ctg cgt tcg aga agg ctg agt gag ttt tct gag ctg ctg atg gaa 931Ala Leu Arg Ser Arg Arg Leu Ser Glu Phe Ser Glu Leu Leu Met Glu
265 270 275tcc caa gat gat ttg agc gac acc ttc gat ttc ccc cct gct gat ttg 979Ser Gln Asp Asp Leu Ser Asp Thr Phe Asp Phe Pro Pro Ala Asp Leu
280 285 290gcg ctt gct cgt ttg tgc gtc gag cgg ggt gcc aca gct gct cgg tcc 1027Ala Leu Ala Arg Leu Cys Val Glu Arg Gly Ala Thr Ala Ala Arg Ser
295 300 305acg tca gcg cgc ggt gtg att gcg ttg gtt gat gcc cat cat gcg cac 1075Thr Ser Ala Arg Gly Val Ile Ala Leu Val Asp Ala His His Ala His310 315 320 325aat ttt gct gcg gat ctc agc gag gat ggc ttg ttg gtg gtt cct ctc 1123Asn Phe Ala Ala Asp Leu Ser Glu Asp Gly Leu Leu Val Val Pro Leu
330 335 340ggg cac ggg gac gtc gcg gaa cag ggc tagcacgcct acttaaccag 1170Gly His Gly Asp Val Ala Glu Gln Gly
345 350cct 1173<210>486<211>350<212>PRT<213>谷氨酸棒杆菌<400>486Met Leu Leu Thr Tyr Ala Phe Val Asp Val Glu Gly Gly Val Glu Lys1 5 10 15His Ser Leu Ser Thr Ala Asp Ile Ala Ala Arg Ala His Ala His Met
20 25 30Lys Ser His Asp Val Leu Gly Arg Gln Thr Thr Pro Pro Gln Pro Glu
35 40 45Gly Gly Val Ala Ala Arg Leu Gly Gly Ile Ala Trp Thr Met Ile His
50 55 60Lys Gln Met Leu Ser Arg Asp Thr Lys Gly Leu Asp Ile Thr Val Leu65 70 75 80Ser Thr Ile Pro Glu Gly Val Gly Leu Gly Glu Asn Ser Ala Met Asp
85 90 95Val Ala Leu Ala Leu Ala Leu Tyr Arg Glu Asn Ile Glu Glu Ala Pro
100 105 110Thr Lys Ala Arg Ile Ala Glu Ile Cys Ser Gln Ser Ala Phe Met Phe
115 120 125Ser Glu Thr Ser Val Leu Arg Ala Arg His Thr Val Ala Leu Arg Gly
130 135 140Glu Thr Gly Gln Ile Ser Val Val Asp Tyr Ala Asp Gly Ser Val Thr145 150 155 160Gln Ala Pro His Pro Val Ser Arg Ser Ala Gly Leu Ser Ala Phe Val
165 170 175Val Ala Ala Gln Thr Glu Thr Asp Pro Ser Ile Tyr Arg Glu Ile Tyr
180 185 190Ala Arg His Ala Phe Ile Asp Glu Ala Ala Arg Ala Phe Ser Val Glu
195 200 205Ser Leu Arg Leu Leu Pro Asp Ala Ser Thr Arg Val Val Asp Trp Leu
210 215 220Gln Ala Val Ile Glu Val Thr Gly Arg Glu Asp Leu Pro Ser Ile Glu225 230 235 240Gln Ala Gln Arg Trp Leu Asn Leu Trp Glu Asn Glu Thr Arg Arg Ala
245 250 255Gln Arg Thr Ala Asn Ala Leu Arg Ser Arg Arg Leu Ser Glu Phe Ser
260 265 270Glu Leu Leu Met Glu Ser Gln Asp Asp Leu Ser Asp Thr Phe Asp Phe
275 280 285Pro Pro Ala Asp Leu Ala Leu Ala Arg Leu Cys Val Glu Arg Gly Ala
290 295 300Thr Ala Ala Arg Ser Thr Ser Ala Arg Gly Val Ile Ala Leu Val Asp305 310 315 320Ala His His Ala His Asn Phe Ala Ala Asp Leu Ser Glu Asp Gly Leu
325 330 335Leu Val Val Pro Leu Gly His Gly Asp Val Ala Glu Gln Gly
340 345 350<210>487<211>1248<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1225)<223>RXS00584<400>487tagttgtgcc acctaaaacg cgaacagaac cggagtcgag cagcacctcc ccgcaagggt 60agaggggctg cttttttgtt tcctaaattc accccatccc atg cat agc cct gaa 115
Met His Ser Pro Glu
1 5agg caa gaa aaa atg agt tct cca gtc tca ctc gaa aac gcg gcg tca 163Arg Gln Glu Lys Met Ser Ser Pro Val Ser Leu Glu Asn Ala Ala Ser
10 15 20acc agc aac aag cgc gtc gtg gct ttc cac gag ctg cct agc cct aca 211Thr Ser Asn Lys Arg Val Val Ala Phe His Glu Leu Pro Ser Pro Thr
25 30 35gat ctc atc gcc gca aac cca ctg aca cca aag cag gct tcc aag gtg 259Asp Leu Ile Ala Ala Asn Pro Leu Thr Pro Lys Gln Ala Ser Lys Val
40 45 50gag cag gat cgc cag gac atc gct gat atc ttc gct ggc gac gat gac 307Glu Gln Asp Arg Gln Asp Ile Ala Asp Ile Phe Ala Gly Asp Asp Asp
55 60 65cgc ctc gtt gtc gtt gtg gga cct tgc tca gtt cac gat cct gaa gca 355Arg Leu Val Val Val Val Gly Pro Cys Ser Val His Asp Pro Glu Ala70 75 80 85gcc atc gat tac gca aac cgc ctg gct ccg ctg gca aag cgc ctt gat 403Ala Ile Asp Tyr Ala Asn Arg Leu Ala Pro Leu Ala Lys Arg Leu Asp
90 95 100cag gac ctc aag att gtc atg cgc gtg tac ttc gag aag cct cgc acc 451Gln Asp Leu Lys Ile Val Met Arg Val Tyr Phe Glu Lys Pro Arg Thr
105 110 115atc gtc gga tgg aag gga ttg atc aat gat cct cac ctc aac gaa acc 499Ile Val Gly Trp Lys Gly Leu Ile Asn Asp Pro His Leu Asn Glu Thr
120 125 130tac gac atc cca gag ggc ttg cgc att gcg cgc aaa gtg ctt atc gac 547Tyr Asp Ile Pro Glu Gly Leu Arg Ile Ala Arg Lys Val Leu Ile Asp
135 140 145gtt gtg aac ctt gat ctc cca gtc ggc tgc gaa ttc ctc gaa cca aac 595Val Val Asn Leu Asp Leu Pro Val Gly Cys Glu Phe Leu Glu Pro Asn150 155 160 165agc cct cag tac tac gcc gac act gtc gca tgg gga gca atc ggc gct 643Ser Pro Gln Tyr Tyr Ala Asp Thr Val Ala Trp Gly Ala Ile Gly Ala
170 175 180cgt acc acc gaa tct cag gtg cac cgc cag ctg gct tct ggg atg tct 691Arg Thr Thr Glu Ser Gln Val His Arg Gln Leu Ala Ser Gly Met Ser
185 190 195atg cca att ggt ttc aag aac gga act gac gga aac atc cag gtt gca 739Met Pro Ile Gly Phe Lys Asn Gly Thr Asp Gly Asn Ile Gln Val Ala
200 205 210gtc gac gcg gta cag gct gcc cag aac cca cac ttc ttc ttc gga acc 787Val Asp Ala Val Gln Ala Ala Gln Asn Pro His Phe Phe Phe Gly Thr
215 220 225tcc gac gac ggc gcg ctg agc gtc gtg gag acc gca ggc aac agc aac 835Ser Asp Asp Gly Ala Leu Ser Val Val Glu Thr Ala Gly Asn Ser Asn230 235 240 245tcc cac atc att ttg cgc ggc ggt acc tcc ggc ccg aat cat gat gca 883Ser His Ile Ile Leu Arg Gly Gly Thr Ser Gly Pro Asn His Asp Ala
250 255 260gct tcg gtg gag gcc gtc gtc gag aag ctt ggt gaa aac gct cgt ctc 931Ala Ser Val Glu Ala Val Val Glu Lys Leu Gly Glu Asn Ala Arg Leu
265 270 275atg atc gat gct tcc cat gct aac tcc ggc aag gat cat atc cga cag 979Met Ile Asp Ala Ser His Ala Asn Ser Gly Lys Asp His Ile Arg Gln
280 285 290gtt gag gtt gtt cgt gaa atc gca gag cag att tct ggc ggt tct gaa 1027Val Glu Val Val Arg Glu Ile Ala Glu Gln Ile Ser Gly Gly Ser Glu
295 300 305gct gtg gct gga atc atg att gag tcc ttc ctc gtt ggt ggc gca cag 1075Ala Val Ala Gly Ile Met Ile Glu Ser Phe Leu Val Gly Gly Ala Gln310 315 320 325aac ctt gat cct gcg aaa ttg cgc atc aat ggc ggt gaa ggc ctg gtg 1123Asn Leu Asp Pro Ala Lys Leu Arg Ile Asn Gly Gly Glu Gly Leu Val
330 335 340tac gga cag tct gtg acc gat aag tgc atc gat att gac acc acc atc 1171Tyr Gly Gln Ser Val Thr Asp Lys Cys Ile Asp Ile Asp Thr Thr Ile
345 350 355gat ttg ctc gct gag ctg gcc gca gca gta agg gaa cgc cga gca gca 1219Asp Leu Leu Ala Glu Leu Ala Ala Ala Val Arg Glu Arg Arg Ala Ala
360 365 370gcc aag taattaaggg cgctagactg tta 1248Ala Lys
375<210>488<211>375<212>PRT<213>谷氨酸棒杆菌<400>488Met His Ser Pro Glu Arg Gln Glu Lys Met Ser Ser Pro Val Ser Leu1 5 10 15Glu Asn Ala Ala Ser Thr Ser Asn Lys Arg Val Val Ala Phe His Glu
20 25 30Leu Pro Ser Pro Thr Asp Leu Ile Ala Ala Asn Pro Leu Thr Pro Lys
35 40 45Gln Ala Ser Lys Val Glu Gln Asp Arg Gln Asp Ile Ala Asp Ile Phe
50 55 60Ala Gly Asp Asp Asp Arg Leu Val Val Val Val Gly Pro Cys Ser Val65 70 75 80His Asp Pro Glu Ala Ala Ile Asp Tyr Ala Asn Arg Leu Ala Pro Leu
85 90 95Ala Lys Arg Leu Asp Gln Asp Leu Lys Ile Val Met Arg Val Tyr Phe
100 105 110Glu Lys Pro Arg Thr Ile Val Gly Trp Lys Gly Leu Ile Asn Asp Pro
115 120 125His Leu Asn Glu Thr Tyr Asp Ile Pro Glu Gly Leu Arg Ile Ala Arg
130 135 140Lys Val Leu Ile Asp Val Val Asn Leu Asp Leu Pro Val Gly Cys Glu145 150 155 160Phe Leu Glu Pro Asn Ser Pro Gln Tyr Tyr Ala Asp Thr Val Ala Trp
165 170 175Gly Ala Ile Gly Ala Arg Thr Thr Glu Ser Gln Val His Arg Gln Leu
180 185 190Ala Ser Gly Met Ser Met Pro Ile Gly Phe Lys Asn Gly Thr Asp Gly
195 200 205Asn Ile Gln Val Ala Val Asp Ala Val Gln Ala Ala Gln Asn Pro His
210 215 220Phe Phe Phe Gly Thr Ser Asp Asp Gly Ala Leu Ser Val Val Glu Thr225 230 235 240Ala Gly Asn Ser Asn Ser His Ile Ile Leu Arg Gly Gly Thr Ser Gly
245 250 255Pro Asn His Asp Ala Ala Ser Val Glu Ala Val Val Glu Lys Leu Gly
260 265 270Glu Asn Ala Arg Leu Met Ile Asp Ala Ser His Ala Asn Ser Gly Lys
275 280 285Asp His Ile Arg Gln Val Glu Val Val Arg Glu Ile Ala Glu Gln Ile
290 295 300Ser Gly Gly Ser Glu Ala Val Ala Gly Ile Met Ile Glu Ser Phe Leu305 310 315 320Val Gly Gly Ala Gln Asn Leu Asp Pro Ala Lys Leu Arg Ile Asn Gly
325 330 335Gly Glu Gly Leu Val Tyr Gly Gln Ser Val Thr Asp Lys Cys Ile Asp
340 345 350Ile Asp Thr Thr Ile Asp Leu Leu Ala Glu Leu Ala Ala Ala Val Arg
355 360 365Glu Arg Arg Ala Ala Ala Lys
370 375<210>489<211>1131<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1108)<223>RXS02574<400>489tgtgctcctt gcgggctgcg cagaagagcc ggaacagcaa aaagcaataa gccgcttatc 60gacgtccccc tccacccctc ccgcaccgac cgcggaggat ttg gcg cgc gcg caa 115
Leu Ala Arg Ala Gln
1 5atc cct gaa cag caa cgc gac caa gtc gcg tcg ctg atg atg gtt gga 163Ile Pro Glu Gln Gln Arg Asp Gln Val Ala Ser Leu Met Met Val Gly
10 15 20gtt gcg aat tat gat cag gca ttg gat gcg ctc aat cag ggg gtg ggt 211Val Ala Asn Tyr Asp Gln Ala Leu Asp Ala Leu Asn Gln Gly Val Gly
25 30 35ggc atc ttt att ggt tcc tgg aca gat gaa aat ctg ctc acg gaa cct 259Gly Ile Phe Ile Gly Ser Trp Thr Asp Glu Asn Leu Leu Thr Glu Pro
40 45 50ggc cgt aat att gag gcg ctc cgc gaa gcc gtc ggc agg gat ttc tcc 307Gly Arg Asn Ile Glu Ala Leu Arg Glu Ala Val Gly Arg Asp Phe Ser
55 60 65gtc agc atc gac ttc gaa ggc ggc cgc gtc cag cgt gcc acc aat att 355Val Ser Ile Asp Phe Glu Gly Gly Arg Val Gln Arg Ala Thr Asn Ile70 75 80 85ctt ggt gat ttc ccc tca ccg cgc gtg atg gcg caa acc atg acg ccg 403Leu Gly Asp Phe Pro Ser Pro Arg Val Met Ala Gln Thr Met Thr Pro
90 95 100gaa caa gta gaa gat ctc gca gaa atc cta ggc act ggt tta gct gca 451Glu Gln Val Glu Asp Leu Ala Glu Ile Leu Gly Thr Gly Leu Ala Ala
105 110 115cat ggt gtg aca gtt aac ttt gca cct gtt gta gat gta gat gct tgg 499His Gly Val Thr Val Asn Phe Ala Pro Val Val Asp Val Asp Ala Trp
120 125 130ggt ctc ccc gtc gtt ggc gat cgt tcc ttt tcc aac gac cca gcc gta 547Gly Leu Pro Val Val Gly Asp Arg Ser Phe Ser Asn Asp Pro Ala Val
135 140 145gca gct act tat gcc aca gct ttt gca aag ggc tta agc aaa gta gga 595Ala Ala Thr Tyr Ala Thr Ala Phe Ala Lys Gly Leu Ser Lys Val Gly150 155 160 165att acc cca gta ttc aaa cat ttc cca ggt cac ggt cgt gca agt ggc 643Ile Thr Pro Val Phe Lys His Phe Pro Gly His Gly Arg Ala Ser Gly
170 175 180gat tcg cac acc caa gat gtg gtg acc ccc gca ctt gat gag ctt aaa 691Asp Ser His Thr Gln Asp Val Val Thr Pro Ala Leu Asp Glu Leu Lys
185 190 195act tac gac ctc atc cct tat ggt caa gca ctt tct gaa act gac gga 739Thr Tyr Asp Leu Ile Pro Tyr Gly Gln Ala Leu Ser Glu Thr Asp Gly
200 205 210gcc gtc atg gtg ggc cac atg att gtt cca ggt ctt ggc acc gac gga 787Ala Val Met Val Gly His Met Ile Val Pro Gly Leu Gly Thr Asp Gly
215 220 225gtt cca tcc tct atc gac ccc gcc acc tat caa ctg ctc cgc agt ggc 835Val Pro Ser Ser Ile Asp Pro Ala Thr Tyr Gln Leu Leu Arg Ser Gly230 235 240 245gat tac cca ggt ggc gtg cct ttc gat ggc gtg atc tac acc gac gat 883Asp Tyr Pro Gly Gly Val Pro Phe Asp Gly Val Ile Tyr Thr Asp Asp
250 255 260ctc tct gga atg agt gcc att tcc gcc acc cat tca ccc gca gaa gca 931Leu Ser Gly Met Ser Ala Ile Ser Ala Thr His Ser Pro Ala Glu Ala
265 270 275gtg ctt gcc tcc ctc aaa gca ggc gca gac caa gca cta tgg atc gac 979Val Leu Ala Ser Leu Lys Ala Gly Ala Asp Gln Ala Leu Trp Ile Asp
280 285 290tat ggg tcg ttg ggc tcc gcg att gat cgc gtt gat gct gcc gtt agc 1027Tyr Gly Ser Leu Gly Ser Ala Ile Asp Arg Val Asp Ala Ala Val Ser
295 300 305agc ggt gaa tac cct caa gaa caa atg ctg gca tct gcg tta aga gtc 1075Ser Gly Glu Tyr Pro Gln Glu Gln Met Leu Ala Ser Ala Leu Arg Val310 315 320 325caa ttg ctc tac atc aca cgt ctc gaa caa aag tgaagttacc agtccgtaac 1128Gln Leu Leu Tyr Ile Thr Arg Leu Glu Gln Lys
330 335ccc 1131<210>490<211>336<212>PRT<213>谷氨酸棒杆菌<400>490Leu Ala Arg Ala Gln Ile Pro Glu Gln Gln Arg Asp Gln Val Ala Ser1 5 10 15Leu Met Met Val Gly Val Ala Asn Tyr Asp Gln Ala Leu Asp Ala Leu
20 25 30Asn Gln Gly Val Gly Gly Ile Phe Ile Gly Ser Trp Thr Asp Glu Asn
35 40 45Leu Leu Thr Glu Pro Gly Arg Asn Ile Glu Ala Leu Arg Glu Ala Val
50 55 60Gly Arg Asp Phe Ser Val Ser Ile Asp Phe Glu Gly Gly Arg Val Gln65 70 75 80Arg Ala Thr Asn Ile Leu Gly Asp Phe Pro Ser Pro Arg Val Met Ala
85 90 95Gln Thr Met Thr Pro Glu Gln Val Glu Asp Leu Ala Glu Ile Leu Gly
100 105 110Thr Gly Leu Ala Ala His Gly Val Thr Val Asn Phe Ala Pro Val Val
115 120 125Asp Val Asp Ala Trp Gly Leu Pro Val Val Gly Asp Arg Ser Phe Ser
130 135 140Asn Asp Pro Ala Val Ala Ala Thr Tyr Ala Thr Ala Phe Ala Lys Gly145 150 155 160Leu Ser Lys Val Gly Ile Thr Pro Val Phe Lys His Phe Pro Gly His
165 170 175Gly Arg Ala Ser Gly Asp Ser His Thr Gln Asp Val Val Thr Pro Ala
180 185 190Leu Asp Glu Leu Lys Thr Tyr Asp Leu Ile Pro Tyr Gly Gln Ala Leu
195 200 205Ser Glu Thr Asp Gly Ala Val Met Val Gly His Met Ile Val Pro Gly
210 215 220Leu Gly Thr Asp Gly Val Pro Ser Ser Ile Asp Pro Ala Thr Tyr Gln225 230 235 240Leu Leu Arg Ser Gly Asp Tyr Pro Gly Gly Val Pro Phe Asp Gly Val
245 250 255Ile Tyr Thr Asp Asp Leu Ser Gly Met Ser Ala Ile Ser Ala Thr His
260 265 270Ser Pro Ala Glu Ala Val Leu Ala Ser Leu Lys Ala Gly Ala Asp Gln
275 280 285Ala Leu Trp Ile Asp Tyr Gly Ser Leu Gly Ser Ala Ile Asp Arg Val
290 295 300Asp Ala Ala Val Ser Ser Gly Glu Tyr Pro Gln Glu Gln Met Leu Ala305 310 315 320Ser Ala Leu Arg Val Gln Leu Leu Tyr Ile Thr Arg Leu Glu Gln Lys
325 330 335<210>491<211>1038<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1008)<223>RXS03215<400>491atc gat gtt gtc agc gtc gtg gtg gct aac ttc ctg cac cgc gaa atc 48Ile Asp Val Val Ser Val Val Val Ala Asn Phe Leu His Arg Glu Ile1 5 10 15gtg gaa gca ctt ctg gca tcc ggc aag cat gtg ctg tgc gag aag cca 96Val Glu Ala Leu Leu Ala Ser Gly Lys His Val Leu Cys Glu Lys Pro
20 25 30ctg tca gac acc atc gaa gat gca gaa gcc atg att gag gca gcc ggc 144Leu Ser Asp Thr Ile Glu Asp Ala Glu Ala Met Ile Glu Ala Ala Gly
35 40 45cgt gca gca aca aat ggc acc atc gcc cgc atc gga ctg acc tac cgc 192Arg Ala Ala Thr Asn Gly Thr Ile Ala Arg Ile Gly Leu Thr Tyr Arg
50 55 60cgt tcc cca ggc gtg gca cac atc cgt gat ctc gtg cag tcc ggc gag 240Arg Ser Pro Gly Val Ala His Ile Arg Asp Leu Val Gln Ser Gly Glu65 70 75 80ctt ggc aag gtt cta cac gtc acc ggc cac tac tgg acc gac tac gga 288Leu Gly Lys Val Leu His Val Thr Gly His Tyr Trp Thr Asp Tyr Gly
85 90 95tcc aat gca cag gca cca atc agc tgg cgt tac aag ggg cca aac ggc 336Ser Asn Ala Gln Ala Pro Ile Ser Trp Arg Tyr Lys Gly Pro Asn Gly
100 105 110tcc ggc gca ctg gca gat gtg gga agc cac ctc acc tac ctg gca gaa 384Ser Gly Ala Leu Ala Asp Val Gly Ser His Leu Thr Tyr Leu Ala Glu
115 120 125ttc gtt gca gga tct gac ttc gcc gcc gtc cgt ggt ggc cag ttg tcc 432Phe Val Ala Gly Ser Asp Phe Ala Ala Val Arg Gly Gly Gln Leu Ser
130 135 140acc gtg atc acc gag cgc ccc aag cca ctc ggc gcg att gtc ggc cac 480Thr Val Ile Thr Glu Arg Pro Lys Pro Leu Gly Ala Ile Val Gly His145 150 155 160gaa ggc ggc gca gtt tcc gat gaa tac gaa gca gtg gaa aat gat gac 528Glu Gly Gly Ala Val Ser Asp Glu Tyr Glu Ala Val Glu Asn Asp Asp
165 170 175att gca tca ttc tcc gga tcc ttc atc ggt ggc gga acc gca acc ctc 576Ile Ala Ser Phe Ser Gly Ser Phe Ile Gly Gly Gly Thr Ala Thr Leu
180 185 190cag gtc agc cgc att tcc cag gga cac cca aac acc cta ggt ttt gaa 624Gln Val Ser Arg Ile Ser Gln Gly His Pro Asn Thr Leu Gly Phe Glu
195 200 205gtg ttc tgt gaa aag ggc tcc gtg ctc ttt gat ttc cgc aac tca ggc 672Val Phe Cys Glu Lys Gly Ser Val Leu Phe Asp Phe Arg Asn Ser Gly
210 215 220gaa ttc aaa atc ttc acc cca gca acc tcc ggt gac atc agc caa gaa 720Glu Phe Lys Ile Phe Thr Pro Ala Thr Ser Gly Asp Ile Ser Gln Glu225 230 235 240gcc ggc tac cgc acc atc acc atc gga cca aag cac cca tac tgg cgc 768Ala Gly Tyr Arg Thr Ile Thr Ile Gly Pro Lys His Pro Tyr Trp Arg
245 250 255ggc ggc ctt gca atg gat gca cca ggc gtg gga att ggc caa aac gaa 816Gly Gly Leu Ala Met Asp Ala Pro Gly Val Gly Ile Gly Gln Asn Glu
260 265 270ggc ttc gtt ttc cag gcg cgc gca ttc ctc gaa gaa atc gca gga atc 864Gly Phe Val Phe Gln Ala Arg Ala Phe Leu Glu Glu Ile Ala Gly Ile
275 280 285tcc gaa gct gaa agc ctg cca cgc tgc gca act ttg gaa gaa ggg cta 912Ser Glu Ala Glu Ser Leu Pro Arg Cys Ala Thr Leu Glu Glu Gly Leu
290 295 300cac aat atg cag ctc att gat gct gta tca cag tca gct gca gca ggt 960His Asn Met Gln Leu Ile Asp Ala Val Ser Gln Ser Ala Ala Ala Gly305 310 315 320ggc gaa acc gtt gcg gtc cca gcg gct gct ctg atc cct gca aac aac 1008Gly Glu Thr Val Ala Val Pro Ala Ala Ala Leu Ile Pro Ala Asn Asn
325 330 335tagaaactat tcagaaagca tcaccatgaa 1038<210>492<211>336<212>PRT<213>谷氨酸棒杆菌<400>492Ile Asp Val Val Ser Val Val Val Ala Asn Phe Leu His Arg Glu Ile1 5 10 15Val Glu Ala Leu Leu Ala Ser Gly Lys His Val Leu Cys Glu Lys Pro
20 25 30Leu Ser Asp Thr Ile Glu Asp Ala Glu Ala Met Ile Glu Ala Ala Gly
35 40 45Arg Ala Ala Thr Asn Gly Thr Ile Ala Arg Ile Gly Leu Thr Tyr Arg
50 55 60Arg Ser Pro Gly Val Ala His Ile Arg Asp Leu Val Gln Ser Gly Glu65 70 75 80Leu Gly Lys Val Leu His Val Thr Gly His Tyr Trp Thr Asp Tyr Gly
85 90 95Ser Asn Ala Gln Ala Pro Ile Ser Trp Arg Tyr Lys Gly Pro Asn Gly
100 105 110Ser Gly Ala Leu Ala Asp Val Gly Ser His Leu Thr Tyr Leu Ala Glu
115 120 125Phe Val Ala Gly Ser Asp Phe Ala Ala Val Arg Gly Gly Gln Leu Ser
130 135 140Thr Val Ile Thr Glu Arg Pro Lys Pro Leu Gly Ala Ile Val Gly His145 150 155 160Glu Gly Gly Ala Val Ser Asp Glu Tyr Glu Ala Val Glu Asn Asp Asp
165 170 175Ile Ala Ser Phe Ser Gly Ser Phe Ile Gly Gly Gly Thr Ala Thr Leu
180 185 190Gln Val Ser Arg Ile Ser Gln Gly His Pro Asn Thr Leu Gly Phe Glu
195 200 205Val Phe Cys Glu Lys Gly Ser Val Leu Phe Asp Phe Arg Asn Ser Gly
210 215 220Glu Phe Lys Ile Phe Thr Pro Ala Thr Ser Gly Asp Ile Ser Gln Glu225 230 235 240Ala Gly Tyr Arg Thr Ile Thr Ile Gly Pro Lys His Pro Tyr Trp Arg
245 250 255Gly Gly Leu Ala Met Asp Ala Pro Gly Val Gly Ile Gly Gln Asn Glu
260 265 270Gly Phe Val Phe Gln Ala Arg Ala Phe Leu Glu Glu Ile Ala Gly Ile
275 280 285Ser Glu Ala Glu Ser Leu Pro Arg Cys Ala Thr Leu Glu Glu Gly Leu
290 295 300His Asn Met Gln Leu Ile Asp Ala Val Ser Gln Ser Ala Ala Ala Gly305 310 315 320Gly Glu Thr Val Ala Val Pro Ala Ala Ala Leu Ile Pro Ala Asn Asn
325 330 335<210>493<211>1031<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1008)<223>FRXA01915<400>493atc gat gtt gtc ago gtc gtg gtg gct aac ttc ctg cac cgc gaa atc 48Ile Asp Val Val Ser Val Val Val Ala Asn Phe Leu His Arg Glu Ile1 5 10 15gtg gaa gca ctt ctg gca tcc ggc aag cat gtg ctg tgc gag aag cca 96Val Glu Ala Leu Leu Ala Ser Gly Lys His Val Leu Cys Glu Lys Pro
20 25 30ctg tca gac acc atc gaa gat gca gaa gcc atg att gag gca gcc ggc 144Leu Ser Asp Thr Ile Glu Asp Ala Glu Ala Met Ile Glu Ala Ala Gly
35 40 45cgt gca gca aca aat ggc acc atc gcc cgc atc gga ctg acc tac cgc 192Arg Ala Ala Thr Asn Gly Thr Ile Ala Arg Ile Gly Leu Thr Tyr Arg
50 55 60cgt tcc cca ggc gtg gca cac atc cgt gat ctc gtg cag tcc ggc gag 240Arg Ser Pro Gly Val Ala His Ile Arg Asp Leu Val Gln Ser Gly Glu65 70 75 80ctt ggc aag gtt cta cac gtc acc ggc cac tac tgg acc gac tac gga 288Leu Gly Lys Val Leu His Val Thr Gly His Tyr Trp Thr Asp Tyr Gly
85 90 95tcc aat gca cag gca cca atc agc tgg cgt tac aag ggg cca aac ggc 336Ser Asn Ala Gln Ala Pro Ile Ser Trp Arg Tyr Lys Gly Pro Asn Gly
100 105 110tcc ggc gca ctg gca gat gtg gga agc cac ctc acc tac ctg gca gaa 384Ser Gly Ala Leu Ala Asp Val Gly Ser His Leu Thr Tyr Leu Ala Glu
115 120 125ttc gtt gca gga tct gac ttc gcc gcc gtc cgt ggt ggc cag ttg tcc 432Phe Val Ala Gly Ser Asp Phe Ala Ala Val Arg Gly Gly Gln Leu Ser
130 135 140acc gtg atc acc gag cgc ccc aag cca ctc ggc gcg att gtc ggc cac 480Thr Val Ile Thr Glu Arg Pro Lys Pro Leu Gly Ala Ile Val Gly His145 150 155 160gaa ggc ggc gca gtt tcc gat gaa tac gaa gca gtg gaa aat gat gac 528Glu Gly Gly Ala Val Ser Asp Glu Tyr Glu Ala Val Glu Asn Asp Asp
165 170 175att gca tca ttc tcc gga tcc ttc atc ggt ggc gga acc gca acc ctc 576Ile Ala Ser Phe Ser Gly Ser Phe Ile Gly Gly Gly Thr Ala Thr Leu
180 185 190cag gtc agc cgc att tcc cag gga cac cca aac acc cta ggt ttt gaa 624Gln Val Ser Arg Ile Ser Gln Gly His Pro Asn Thr Leu Gly Phe Glu
195 200 205gtg ttc tgt gaa aag ggc tcc gtg ctc ttt gat ttc cgc aac tca ggc 672Val Phe Cys Glu Lys Gly Ser Val Leu Phe Asp Phe Arg Asn Ser Gly
210 215 220gaa ttc aaa atc ttc acc cca gca acc tcc ggt gac atc agc caa gaa 720Glu Phe Lys Ile Phe Thr Pro Ala Thr Ser Gly Asp Ile Ser Gln Glu225 230 235 240gcc ggc tac cgc acc atc acc atc gga cca aag cac cca tac tgg cgc 768Ala Gly Tyr Arg Thr Ile Thr Ile Gly Pro Lys His Pro Tyr Trp Arg
245 250 255ggc ggc ctt gca atg gat gca cca ggc gtg gga att ggc caa aac gaa 816Gly Gly Leu Ala Met Asp Ala Pro Gly Val Gly Ile Gly Gln Asn Glu
260 265 270ggc ttc gtt ttc cag gcg cgc gca ttc ctc gaa gaa atc gca gga atc 864Gly Phe Val Phe Gln Ala Arg Ala Phe Leu Glu Glu Ile Ala Gly Ile
275 280 285tcc gaa gct gaa agc ctg cca cgc tgc gca act ttg gaa gaa ggg cta 912Ser Glu Ala Glu Ser Leu Pro Arg Cys Ala Thr Leu Glu Glu Gly Leu
290 295 300cac aat atg cag ctc att gat gct gta tca cag tca gct gca gca ggt 960His Asn Met Gln Leu Ile Asp Ala Val Ser Gln Ser Ala Ala Ala Gly305 310 315 320ggc gaa acc gtt gcg gtc cca gcg gct gct ctg atc cct gca aac aac 1008Gly Glu Thr Val Ala Val Pro Ala Ala Ala Leu Ile Pro Ala Asn Asn
325 330 335tagaaactat tcagaaagca tca 1031<210>494<211>336<212>PRT<213>谷氨酸棒杆菌<400>494Ile Asp Val Val Ser Val Val Val Ala Asn Phe Leu His Arg Glu Ile1 5 10 15Val Glu Ala Leu Leu Ala Ser Gly Lys His Val Leu Cys Glu Lys Pro
20 25 30Leu Ser Asp Thr Ile Glu Asp Ala Glu Ala Met Ile Glu Ala Ala Gly
35 40 45Arg Ala Ala Thr Asn Gly Thr Ile Ala Arg Ile Gly Leu Thr Tyr Arg
50 55 60Arg Ser Pro Gly Val Ala His Ile Arg Asp Leu Val Gln Ser Gly Glu65 70 75 80Leu Gly Lys Val Leu His Val Thr Gly His Tyr Trp Thr Asp Tyr Gly
85 90 95Ser Asn Ala Gln Ala Pro Ile Ser Trp Arg Tyr Lys Gly Pro Asn Gly
100 105 110Ser Gly Ala Leu Ala Asp Val Gly Ser His Leu Thr Tyr Leu Ala Glu
115 120 125Phe Val Ala Gly Ser Asp Phe Ala Ala Val Arg Gly Gly Gln Leu Ser
130 135 140Thr Val Ile Thr Glu Arg Pro Lys Pro Leu Gly Ala Ile Val Gly His145 150 155 160Glu Gly Gly Ala Val Ser Asp Glu Tyr Glu Ala Val Glu Asn Asp Asp
165 170 175Ile Ala Ser Phe Ser Gly Ser Phe Ile Gly Gly Gly Thr Ala Thr Leu
180 185 190Gln Val Ser Arg Ile Ser Gln Gly His Pro Asn Thr Leu Gly Phe Glu
195 200 205Val Phe Cys Glu Lys Gly Ser Val Leu Phe Asp Phe Arg Asn Ser Gly
210 215 220Glu Phe Lys Ile Phe Thr Pro Ala Thr Ser Gly Asp Ile Ser Gln Glu225 230 235 240Ala Gly Tyr Arg Thr Ile Thr Ile Gly Pro Lys His Pro Tyr Trp Arg
245 250 255Gly Gly Leu Ala Met Asp Ala Pro Gly Val Gly Ile Gly Gln Asn Glu
260 265 270Gly Phe Val Phe Gln Ala Arg Ala Phe Leu Glu Glu Ile Ala Gly Ile
275 280 285Ser Glu Ala Glu Ser Leu Pro Arg Cys Ala Thr Leu Glu Glu Gly Leu
290 295 300His Asn Met Gln Leu Ile Asp Ala Val Ser Gln Ser Ala Ala Ala Gly305 310 315 320Gly Glu Thr Val Ala Val Pro Ala Ala Ala Leu Ile Pro Ala Asn Asn
325 330 335<210>495<211>1288<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1258)<223>RXS03224<400>495acgattgtgc tgtcgtttgc gttggtgaat agttctggac cgggtatttt gcggcgcaca 60tggaactcat tgaacgccgc gcccggctaa ggtgggaggc atg agt ttt gct gaa 115
Met Ser Phe Ala Glu
1 5cat gcg atc atc tgg cac gtc tac ccc ctg ggc gct ttg ggt gct ccc 163His Ala Ile Ile Trp His Val Tyr Pro Leu Gly Ala Leu Gly Ala Pro
10 15 20atc cgg cct gaa gcc ccc gca cct gtc aca cat cgg ctc ccc aat cta 211Ile Arg Pro Glu Ala Pro Ala Pro Val Thr His Arg Leu Pro Asn Leu
25 30 35att ggg tgg ctg gat tat gtt gtc gaa cta ggc tgc aac gcc ctc atg 259Ile Gly Trp Leu Asp Tyr Val Val Glu Leu Gly Cys Asn Ala Leu Met
40 45 50ctg gga ccg gta ttc gag tcc gtc agc cac ggc tac gac acc ctc gat 307Leu Gly Pro Val Phe Glu Ser Val Ser His Gly Tyr Asp Thr Leu Asp
55 60 65ttc tac cgc atc gac ccg cgc ctc ggc acc gag gaa gac atg gac gcg 355Phe Tyr Arg Ile Asp Pro Arg Leu Gly Thr Glu Glu Asp Met Asp Ala70 75 80 85ctg ctg gag gct gcg aat cag cgg ggc att gga gtg ctt ttc gac ggc 403Leu Leu Glu Ala Ala Asn Gln Arg Gly Ile Gly Val Leu Phe Asp Gly
90 95 100gtc ttc aat cat gtt tcc agt tcc tct aaa tat ctc gac ctg acc acc 451Val Phe Asn His Val Ser Ser Ser Ser Lys Tyr Leu Asp Leu Thr Thr
105 110 115ggg gcg tca ttt gaa ggc cac gac atc ctg gcg gaa ctc gac cac acg 499Gly Ala Ser Phe Glu Gly His Asp Ile Leu Ala Glu Leu Asp His Thr
120 125 130aat ccc gcc gta gtg gat ctg gtt gtc gat gtc atg aac cac tgg ctc 547Asn Pro Ala Val Val Asp Leu Val Val Asp Val Met Asn His Trp Leu
135 140 145gac cgc gga atc gca ggc tgg cga ctc gac gct gtc tac gcc atc gcc 595Asp Arg Gly Ile Ala Gly Trp Arg Leu Asp Ala Val Tyr Ala Ile Ala150 155 160 165cct gaa ttt tgg gaa aaa gtc ctg cca gaa gtg cga cga aaa cac cca 643Pro Glu Phe Trp Glu Lys Val Leu Pro Glu gal Arg Arg Lys His Pro
170 175 180cac gca tgg atc gtg ggg gag atg atc cat gga gat tac tcc gac tac 691His Ala Trp Ile Val Gly Glu Met Ile His Gly Asp Tyr Ser Asp Tyr
185 190 195gtg aaa agc tcc ggc att gat tcc gtt acc gaa tac gaa ctg tgg aaa 739Val Lys Ser Ser Gly Ile Asp Ser Val Thr Glu Tyr Glu Leu Trp Lys
200 205 210gcc att tgg agc agc atc aaa gag cgc aat ttc ttt gaa ctc gaa tgg 787Ala Ile Trp ser Ser Ile Lys Glu Arg Asn Phe Phe Glu Leu Glu Trp
215 220 225act ttg agt cgc cac aat gaa ttc ctc gat act ttc gta ccg cag aca 835Thr Leu Ser Arg His Asn Glu Phe Leu Asp Thr Phe Val Pro Gln Thr230 235 240 245ttc att ggt aac cat gac gtc acc cgc att gcc acc cga atc ggt caa 883Phe Ile Gly Asn His Asp Val Thr Arg Ile Ala Thr Arg Ile Gly Gln
250 255 260tca aat gcg atc ctg gcc gca gcg atc ctc ttc acg gtc gga gga acc 931Ser Asn Ala Ile Leu Ala Ala Ala Ile Leu Phe Thr Val Gly Gly Thr
265 270 275cca agc att tac tac ggc gat gag cag ggc ttt acg gga ttg aaa gag 979Pro Ser Ile Tyr Tyr Gly Asp Glu Gln Gly Phe Thr Gly Leu Lys Glu
280 285 290gat aac gtt ttc ggt gac gat gcc att agg cca cct ctt cct gcc gag 1027Asp Asn Val Phe Gly Asp Asp Ala Ile Arg Pro Pro Leu Pro Ala Glu
295 300 305ttt tct cca ctg ggc acc tgg att gaa aac att tat aag gct ctg atc 1075Phe Ser Pro Leu Gly Thr Trp Ile Glu Asn Ile Tyr Lys Ala Leu Ile310 315 320 325gcg ctg cgc agg caa cac ccg tgg ttg tat cag gcg cac acc gaa gtc 1123Ala Leu Arg Arg Gln His Pro Trp Leu Tyr Gln Ala His Thr Glu Val
330 335 340ctt gag att gct aat gaa gcg atg acc tat aag tcc gtc ggt ctt gga 1171Leu Glu Ile Ala Asn Glu Ala Met Thr Tyr Lys Ser Val Gly Leu Gly
345 350 355ggt gaa gag ctg aca gtg cat ctt gat ttg gaa gag gtg tct gtt cgg 1219Gly Glu Glu Leu Thr Val His Leu Asp Leu Glu Glu Val Ser Val Arg
360 365 370atc ctt gat ggc gag aag gtg ctg ttt cag tac agc gct tagttgtcgg 1268Ile Leu Asp Gly Glu Lys Val Leu Phe Gln Tyr Ser Ala
375 380 385ttcaagggta ggggaacaaa 1288<210>496<211>386<212>PRT<213>谷氨酸棒杆菌<400>496Met Ser Phe Ala Glu His Ala Ile Ile Trp His Val Tyr Pro Leu Gly1 5 10 15Ala Leu Gly Ala Pro Ile Arg Pro Glu Ala Pro Ala Pro Val Thr His
20 25 30Arg Leu Pro Asn Leu Ile Gly Trp Leu Asp Tyr Val Val Glu Leu Gly
35 40 45Cys Asn Ala Leu Met Leu Gly Pro Val Phe Glu Ser Val Ser His Gly
50 55 60Tyr Asp Thr Leu Asp Phe Tyr Arg Ile Asp Pro Arg Leu Gly Thr Glu65 70 75 80Glu Asp Met Asp Ala Leu Leu Glu Ala Ala Asn Gln Arg Gly Ile Gly
85 90 95Val Leu Phe Asp Gly Val Phe Asn His Val Ser Ser Ser Ser Lys Tyr
100 105 110Leu Asp Leu Thr Thr Gly Ala Ser Phe Glu Gly His Asp Ile Leu Ala
115 120 125Glu Leu Asp His Thr Asn Pro Ala Val Val Asp Leu Val Val Asp Val
130 135 140Met Asn His Trp Leu Asp Arg Gly Ile Ala Gly Trp Arg Leu Asp Ala145 150 155 160Val Tyr Ala Ile Ala Pro Glu Phe Trp Glu Lys Val Leu Pro Glu Val
165 170 175Arg Arg Lys His Pro His Ala Trp Ile Val Gly Glu Met Ile His Gly
180 185 190Asp Tyr Ser Asp Tyr Val Lys Ser Ser Gly Ile Asp Ser Val Thr Glu
195 200 205Tyr Glu Leu Trp Lys Ala Ile Trp Ser Ser Ile Lys Glu Arg Asn Phe
210 215 220Phe Glu Leu Glu Trp Thr Leu Ser Arg His Asn Glu Phe Leu Asp Thr225 230 235 240Phe Val Pro Gln Thr Phe Ile Gly Asn His Asp Val Thr Arg Ile Ala
245 250 255Thr Arg Ile Gly Gln Ser Asn Ala Ile Leu Ala Ala Ala Ile Leu Phe
260 265 270Thr Val Gly Gly Thr Pro Ser Ile Tyr Tyr Gly Asp Glu Gln Gly Phe
275 280 285Thr Gly Leu Lys Glu Asp Asn Val Phe Gly Asp Asp Ala Ile Arg Pro
290 295 300Pro Leu Pro Ala Glu Phe Ser Pro Leu Gly Thr Trp Ile Glu Asn Ile305 310 315 320Tyr Lys Ala Leu Ile Ala Leu Arg Arg Gln His Pro Trp Leu Tyr Gln
325 330 335Ala His Thr Glu Val Leu Glu Ile Ala Asn Glu Ala Met Thr Tyr Lys
340 345 350Ser Val Gly Leu Gly Gly Glu Glu Leu Thr Val His Leu Asp Leu Glu
355 360 365Glu Val Ser Val Arg Ile Leu Asp Gly Glu Lys Val Leu Phe Gln Tyr
370 375 380Ser Ala385<210>497<21l>1281<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1258)<223>FRXA00038<400>497acgattgtgc tgtcgtttgc gttggtgaat agttctggac cgggtatttt gcggcgcaca 60tggaactcat tgaacgccgc gcccggctaa ggtgggaggc atg agt ttt gct gaa 115
Met Ser Phe Ala Glu
1 5cat gcg atc atc tgg cac gtc tac ccc ctg ggc gct ttg ggt gct ccc 163His Ala Ile Ile Trp His Val Tyr Pro Leu Gly Ala Leu Gly Ala Pro
10 15 20atc cgg cct gaa gcc ccc gca cct gtc aca cat cgg ctc ccc aat cta 211Ile Arg Pro Glu Ala Pro Ala Pro Val Thr His Arg Leu Pro Asn Leu
25 30 35att ggg tgg ctg gat tat gtt gtc gaa cta ggc tgc aac gcc ctc atg 259Ile Gly Trp Leu Asp Tyr Val Val Glu Leu Gly Cys Asn Ala Leu Met
40 45 50ctg gga ccg gta ttc gag tcc gtc agc cac ggc tac gac acc ctc gat 307Leu Gly Pro Val Phe Glu Ser Val Ser His Gly Tyr Asp Thr Leu Asp
55 60 65ttc tac cgc atc gac ccg cgc ctc ggc acc gag gaa gac atg gac gcg 355Phe Tyr Arg Ile Asp Pro Arg Leu Gly Thr Glu Glu Asp Met Asp Ala70 75 80 85ctg ctg gag gct gcg aat cag cgg ggc att gga gtg ctt ttc gac ggc 403Leu Leu Glu Ala Ala Asn Gln Arg Gly Ile Gly Val Leu Phe Asp Gly
90 95 100gtc ttc aat cat gtt tcc agt tcc tct aaa tat ctc gac ctg acc acc 451Val Phe Asn His Val Ser Ser Ser Ser Lys Tyr Leu Asp Leu Thr Thr
105 110 115ggg gcg tca ttt gaa ggc cac gac atc ctg gcg gaa ctc gac cac acg 499Gly Ala Ser Phe Glu Gly His Asp Ile Leu Ala Glu Leu Asp His Thr
120 125 130aat ccc gcc gta gtg gat ctg gtt gtc gat gtc atg aac cac tgg ctc 547Asn Pro Ala Val Val Asp Leu Val Val Asp Val Met Asn His Trp Leu
135 140 145gac cgc gga atc gca ggc tgg cga ctc gac gct gtc tac gcc atc gcc 595Asp Arg Gly Ile Ala Gly Trp Arg Leu Asp Ala Val Tyr Ala Ile Ala150 155 160 165cct gaa ttt tgg gaa aaa gtc ctg cca gaa gtg cga cga aaa cac cca 643Pro Glu Phe Trp Glu Lys Val Leu Pro Glu Val Arg Arg Lys His Pro
170 175 180cac gca tgg atc gtg ggg gag atg atc cat gga gat tac tcc gac tac 691His Ala Trp Ile Val Gly Glu Met Ile His Gly Asp Tyr Ser Asp Tyr
185 190 195gtg aaa agc tcc ggc att gat tcc gtt acc gaa tac gaa ctg tgg aaa 739Val Lys Ser Ser Gly Ile Asp Ser Val Thr Glu Tyr Glu Leu Trp Lys
200 205 210gcc att tgg agc agc atc aaa gag cgc aat ttc ttt gaa ctc gaa tgg 787Ala Ile Trp Ser Ser Ile Lys Glu Arg Asn Phe Phe Glu Leu Glu Trp
215 220 225act ttg agt cgc cac aat gaa ttc ctc gat act ttc gta ccg cag aca 835Thr Leu Ser Arg His Asn Glu Phe Leu Asp Thr Phe Val Pro Gln Thr230 235 240 245ttc att ggt aac cat gac gtc acc cgc att gcc acc cga atc ggt caa 883Phe Ile Gly Asn His Asp Val Thr Arg Ile Ala Thr Arg Ile Gly Gln
250 255 260tca aat gcg atc ctg gcc gca gcg atc ctc ttc acg gtc gga gga acc 931Ser Asn Ala Ile Leu Ala Ala Ala Ile Leu Phe Thr Val Gly Gly Thr
265 270 275cca agc att tac tac ggc gat gag cag ggc ttt acg gga ttg aaa gag 979Pro Ser Ile Tyr Tyr Gly Asp Glu Gln Gly Phe Thr Gly Leu Lys Glu
280 285 290gat aac gtt ttc ggt gac gat gcc att agg cca cct ctt cct gcc gag 1027Asp Asn Val Phe Gly Asp Asp Ala Ile Arg Pro Pro Leu Pro Ala Glu
295 300 305ttt tct cca ctg ggc acc tgg att gaa aac att tat aag gct ctg atc 1075Phe Ser Pro Leu Gly Thr Trp Ile Glu Asn Ile Tyr Lys Ala Leu Ile310 315 320 325gcg ctg cgc agg caa cac ccg tgg ttg tat cag gcg cac acc gaa gtc 1123Ala Leu Arg Arg Gln His Pro Trp Leu Tyr Gln Ala His Thr Glu Val
330 335 340ctt gag att gct aat gaa gcg atg acc tat aag tcc gtc ggt ctt gga 1171Leu Glu Ile Ala Asn Glu Ala Met Thr Tyr Lys Ser Val Gly Leu Gly
345 350 355ggt gaa gag ctg aca gtg cat ctt gat ttg gaa gag gtg tct gtt cgg 1219Gly Glu Glu Leu Thr Val His Leu Asp Leu Glu Glu Val Ser Val Arg
360 365 370atc ctt gat ggc gag aag gtg ctg ttt cag tac agc gct tagttgtcgg 1268Ile Leu Asp Gly Glu Lys Val Leu Phe Gln Tyr Ser Ala
375 380 385ttcaagggta ggg 1281<210>498<211>386<212>PRT<213>谷氨酸棒杆菌<400>498Met Ser Phe Ala Glu His Ala Ile Ile Trp His Val Tyr Pro Leu Gly1 5 10 15Ala Leu Gly Ala Pro Ile Arg Pro Glu Ala Pro Ala Pro Val Thr His
20 25 30Arg Leu Pro Asn Leu Ile Gly Trp Leu Asp Tyr Val Val Glu Leu Gly
35 40 45Cys Asn Ala Leu Met Leu Gly Pro Val Phe Glu Ser Val Ser His Gly
50 55 60Tyr Asp Thr Leu Asp Phe Tyr Arg Ile Asp Pro Arg Leu Gly Thr Glu65 70 75 80Glu Asp Met Asp Ala Leu Leu Glu Ala Ala Asn Gln Arg Gly Ile Gly
85 90 95Val Leu Phe Asp Gly Val Phe Asn His Val Ser Ser Ser Ser Lys Tyr
100 105 110Leu Asp Leu Thr Thr Gly Ala Ser Phe Glu Gly His Asp Ile Leu Ala
115 120 125Glu Leu Asp His Thr Asn Pro Ala Val Val Asp Leu Val Val Asp Val
130 135 140Met Asn His Trp Leu Asp Arg Gly Ile Ala Gly Trp Arg Leu Asp Ala145 150 155 160Val Tyr Ala Ile Ala Pro Glu Phe Trp Glu Lys Val Leu Pro Glu Val
165 170 175Arg Arg Lys His Pro His Ala Trp Ile Val Gly Glu Met Ile His Gly
180 185 190Asp Tyr Ser Asp Tyr Val Lys Ser Ser Gly Ile Asp Ser Val Thr Glu
195 200 205Tyr Glu Leu Trp Lys Ala Ile Trp Ser Ser Ile Lys Glu Arg Asn Phe
210 215 220Phe Glu Leu Glu Trp Thr Leu Ser Arg His Asn Glu Phe Leu Asp Thr225 230 235 240Phe Val Pro Gln Thr Phe Ile Gly Asn His Asp Val Thr Arg Ile Ala
245 250 255Thr Arg Ile Gly Gln Ser Asn Ala Ile Leu Ala Ala Ala Ile Leu Phe
260 265 270Thr Val Gly Gly Thr Pro Ser Ile Tyr Tyr Gly Asp Glu Gln Gly Phe
275 280 285Thr Gly Leu Lys Glu Asp Asn Val Phe Gly Asp Asp Ala Ile Arg Pro
290 295 300Pro Leu Pro Ala Glu Phe Ser Pro Leu Gly Thr Trp Ile Glu Asn Ile305 310 315 320Tyr Lys Ala Leu Ile Ala Leu Arg Arg Gln His Pro Trp Leu Tyr Gln
325 330 335Ala His Thr Glu Val Leu Glu Ile Ala Asn Glu Ala Met Thr Tyr Lys
340 345 350Ser Val Gly Leu Gly Gly Glu Glu Leu Thr Val His Leu Asp Leu Glu
355 360 365Glu Val Ser Val Arg Ile Leu Asp Gly Glu Lys Val Leu Phe Gln Tyr
370 375 380Ser Ala385<210>499<211>517<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(517)<223>RXC00233<400>499cgcctccagc agttgaggga gaagttccaa cacttgcacc aactgaggaa gcaactgtgc 60aatagcgctt tagacacaga ctcatgacag aatagaagac atg agt gtg aat gaa 115
Met Ser Val Asn Glu
1 5gca gat ctg aac gct gtc gaa gag caa ttg gga agg gcc cca cga ggt 163Ala Asp Leu Asn Ala Val Glu Glu Gln Leu Gly Arg Ala Pro Arg Gly
10 15 20gtc ctc gat att tct tac cgc agc cct gat gga gta ccc ggt gtg gtg 211Val Leu Asp Ile Ser Tyr Arg Ser Pro Asp Gly Val Pro Gly Val Val
25 30 35atg acc gca cca aaa ctg gat gac gga acc cca ttc cca acc ctg tac 259Met Thr Ala Pro Lys Leu Asp Asp Gly Thr Pro Phe Pro Thr Leu Tyr
40 45 50tac ttg aca gat cca cgc ctg acc acc gag gca tcc cgc ctc gag gtc 307Tyr Leu Thr Asp Pro Arg Leu Thr Thr Glu Ala Ser Arg Leu Glu Val
55 60 65gca ttg gta atg aag tgg atg act gat cgc ctt tcc acc gac gaa gag 355Ala Leu Val Met Lys Trp Met Thr Asp Arg Leu Ser Thr Asp Glu Glu70 75 80 85ctt cgt gcc gac tac cag cgc gcc cac gag cac ttc ctg gca aag cgc 403Leu Arg Ala Asp Tyr Gln Arg Ala His Glu His Phe Leu Ala Lys Arg
90 95 100aac gca att gaa gat ctc ggc acg gat ttt tcc ggc ggt ggc atg cct 451Asn Ala Ile Glu Asp Leu Gly Thr Asp Phe Ser Gly Gly Gly Met Pro
105 110 115gac cga gtg aag tgc ctt cac gtc ctc att gac tat gca ctg gca gaa 499Asp Arg Val Lys Cys Leu His Val Leu Ile Asp Tyr Ala Leu Ala Glu
120 125 130ggc cca cac cat ttc ctt 517Gly Pro His His Phe Leu
135<210>500<21l>139<212>PRT<213>谷氨酸棒杆菌<400>500Met Ser Val Asn Glu Ala Asp Leu Asn Ala Val Glu Glu Gln Leu Gly1 5 10 15Arg Ala Pro Arg Gly Val Leu Asp Ile Ser Tyr Arg Ser Pro Asp Gly
20 25 30Val Pro Gly Val Val Met Thr Ala Pro Lys Leu Asp Asp Gly Thr Pro
35 40 45Phe Pro Thr Leu Tyr Tyr Leu Thr Asp Pro Arg Leu Thr Thr Glu Ala
50 55 60Ser Arg Leu Glu Val Ala Leu Val Met Lys Trp Met Thr Asp Arg Leu65 70 75 80Ser Thr Asp Glu Glu Leu Arg Ala Asp Tyr Gln Arg Ala His Glu His
85 90 95Phe Leu Ala Lys Arg Asn Ala Ile Glu Asp Leu Gly Thr Asp Phe Ser
100 105 110Gly Gly Gly Met Pro Asp Arg Val Lys Cys Leu His Val Leu Ile Asp
115 120 125Tyr Ala Leu Ala Glu Gly Pro His His Phe Leu
130 135<210>501<211>849<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(826)<223>RXC00236<400>501aatgcgagag ttctaaaacg agccggtaac atcgaccccc atgagttcag gggttagaaa 60agcaatggga tttggatgcg gttcggtttt ggccgtcatc atg gtg atc tca ttt 115
Met Val Ile Ser Phe
1 5gtt gga tgg gcg ctc agc ttc atg gat gga acg gca cct att cgc caa 163Val Gly Trp Ala Leu Ser Phe Met Asp Gly Thr Ala Pro Ile Arg Gln
10 15 20ctc cag caa atc cct gaa gat gtt ccg ccg gcg cgt ggt gta gaa gtt 211Leu Gln Gln Ile Pro Glu Asp Val Pro Pro Ala Arg Gly Val Glu Val
25 30 35ccg caa att gat aca gag gca gat gga cgc aca tcc aac cat ttg cgt 259Pro Gln Ile Asp Thr Glu Ala Asp Gly Arg Thr Ser Asn His Leu Arg
40 45 50ttt tgg gcg gaa cca att gct caa gat act ggt gtg tcc gct caa gcg 307Phe Trp Ala Glu Pro Ile Ala Gln Asp Thr Gly Val Ser Ala Gln Ala
55 60 65att gcg gct tat gga aac gca gag ctc atc gcg agt act gcg tgg cct 355Ile Ala Ala Tyr Gly Asn Ala Glu Leu Ile Ala Ser Thr Ala Trp Pro70 75 80 85ggc tgc aat ctg ggg tgg aat acc ttg gca ggt atc ggc cag gtg gaa 403Gly Cys Asn Leu Gly Trp Asn Thr Leu Ala Gly Ile Gly Gln Val Glu
90 95 100acc cgt cac ggt acc tac aac ggc aaa atg ttc ggg ggc agt tcc ctg 451Thr Arg His Gly Thr Tyr Asn Gly Lys Met Phe Gly Gly Ser Ser Leu
105 110 115gat gaa aat gga gtt gca acc cct cca atc atc ggc gtt cca ctt gat 499Asp Glu Asn Gly Val Ala Thr Pro Pro Ile Ile Gly Val Pro Leu Asp
120 125 130ggt tca ccg ggg ttt gcg gaa att ccc gac act gat ggt ggg gaa tta 547Gly Ser Pro Gly Phe Ala Glu Ile Pro Asp Thr Asp Gly Gly Glu Leu
135 140 145gat ggc gat act gaa tat gat cgc gcg gta ggt ccc atg cag ttc att 595Asp Gly Asp Thr Glu Tyr Asp Arg Ala Val Gly Pro Met Gln Phe Ile150 155 160 165ccg gaa acg tgg cga ctt atg gga ttg gat gca aac ggt gat ggg gta 643Pro Glu Thr Trp Arg Leu Met Gly Leu Asp Ala Asn Gly Asp Gly Val
170 175 180gcg gac ccc aac caa att gat gac gca gca ttg agt gcc gca aac ctg 691Ala Asp Pro Asn Gln Ile Asp Asp Ala Ala Leu Ser Ala Ala Asn Leu
185 190 195ttg tgt tcc aac gat cgt gac ttg tcc act cct gaa gga tgg acc gca 739Leu Cys Ser Asn Asp Arg Asp Leu Ser Thr Pro Glu Gly Trp Thr Ala
200 205 210gct gtt cat tct tac aac atg tct aat cag tat ttg atg gac gtt cga 787Ala Val His Ser Tyr Asn Met Ser Asn Gln Tyr Leu Met Asp Val Arg
215 220 225gat gct gcc gcg tcc tac gct tta cga cag ccg gcg atc taaaacttaa 836Asp Ala Ala Ala Ser Tyr Ala Leu Arg Gln Pro Ala Ile230 235 240caagcgcaac ccc 849<210>502<211>242<212>PRT<213>谷氨酸棒杆菌<400>502Met Val Ile Ser Phe Val Gly Trp Ala Leu Ser Phe Met Asp Gly Thr1 5 10 15Ala Pro Ile Arg Gln Leu Gln Gln Ile Pro Glu Asp Val Pro Pro Ala
20 25 30Arg Gly Val Glu Val Pro Gln Ile Asp Thr Glu Ala Asp Gly Arg Thr
35 40 45Ser Asn His Leu Arg Phe Trp Ala Glu Pro Ile Ala Gln Asp Thr Gly
50 55 60Val Ser Ala Gln Ala Ile Ala Ala Tyr Gly Asn Ala Glu Leu Ile Ala65 70 75 80Ser Thr Ala Trp Pro Gly Cys Asn Leu Gly Trp Asn Thr Leu Ala Gly
85 90 95Ile Gly Gln Val Glu Thr Arg His Gly Thr Tyr Asn Gly Lys Met Phe
100 105 110Gly Gly Ser Ser Leu Asp Glu Asn Gly Val Ala Thr Pro Pro Ile Ile
115 120 125Gly Val Pro Leu Asp Gly Ser Pro Gly Phe Ala Glu Ile Pro Asp Thr
130 135 140Asp Gly Gly Glu Leu Asp Gly Asp Thr Glu Tyr Asp Arg Ala Val Gly145 150 155 160Pro Met Gln Phe Ile Pro Glu Thr Trp Arg Leu Met Gly Leu Asp Ala
165 170 175Asn Gly Asp Gly Val Ala Asp Pro Asn Gln Ile Asp Asp Ala Ala Leu
180 185 190Ser Ala Ala Asn Leu Leu Cys Ser Asn Asp Arg Asp Leu Ser Thr Pro
195 200 205Glu Gly Trp Thr Ala Ala Val His Ser Tyr Asn Met Ser Asn Gln Tyr
210 215 220Leu Met Asp Val Arg Asp Ala Ala Ala Ser Tyr Ala Leu Arg Gln Pro225 230 235 240Ala Ile<210>503<211>1113<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1090)<223>RXC00271<400>503tagtttaaat catgagacat ttcacatatg gttctttatc cgagacatgt gttgacgctg 60tctgcccctt tttgaaaata acactttaag gagatgtgcc atg ttt tct tcc cgt 115
Met Phe Ser Ser Arg
1 5tcg aag gta ctc gca agc atc ttt act gtt ggc gcc ttg gcg ttg gct 163Ser Lys Val Leu Ala Ser Ile Phe Thr Val Gly Ala Leu Ala Leu Ala
10 15 20tcg tgc tca agc gat tcc agt gac agc tcc acc tcc act gat gct gca 211Ser Cys Ser Ser Asp Ser Ser Asp Ser Ser Thr Ser Thr Asp Ala Ala
25 30 35ggt ggc gac tct tac cga gtt ggc atc aac cag ctt gtt cag cac cct 259Gly Gly Asp Ser Tyr Arg Val Gly Ile Asn Gln Leu Val Gln His Pro
40 45 50gca ctt gat gca gcg acc act ggt ttc aag gaa gct ttt gaa gag gca 307Ala Leu Asp Ala Ala Thr Thr Gly Phe Lys Glu Ala Phe Glu Glu Ala
55 60 65ggc gtt gac gtc acc ttt gat gag caa aac gct aac ggc gag cag ggc 355Gly Val Asp Val Thr Phe Asp Glu Gln Asn Ala Asn Gly Glu Gln Gly70 75 80 85act gca ctg act att tct cag cag ttc gct tct gac aat ttg gat ctc 403Thr Ala Leu Thr Ile Ser Gln Gln Phe Ala Ser Asp Asn Leu Asp Leu
90 95 100gtg ttg gct gtt gca act cca gca gca cag gca act gcg cag aat atc 451Val Leu Ala Val Ala Thr Pro Ala Ala Gln Ala Thr Ala Gln Asn Ile
105 110 115act gat atc cca gtc ctg ttc acc gca gtt acc gat gca gtg tcg gca 499Thr Asp Ile Pro Val Leu Phe Thr Ala Val Thr Asp Ala Val Ser Ala
120 125 130gag ctg gtg gat tct aat gaa gca cct ggc gga aac gtc acc ggt act 547Glu Leu Val Asp Ser Asn Glu Ala Pro Gly Gly Asn Val Thr Gly Thr
135 140 145tct gat atc gca ccg att gag cag cag ttg gag ctt ttg cag cag ctg 595Ser Asp Ile Ala Pro Ile Glu Gln Gln Leu Glu Leu Leu Gln Gln Leu150 155 160 165gtt cct gac gca aag tcc atc ggc atc gtc tac gcg tct ggt gag gtc 643Val Pro Asp Ala Lys Ser Ile Gly Ile Val Tyr Ala Ser Gly Glu Val
170 175 180aac tct cag gtg cag gtc gat gag gtc acc aag gct gct gag cca ctg 691Asn Ser Gln Val Gln Val Asp Glu Val Thr Lys Ala Ala Glu Pro Leu
185 190 195ggg ctg tcc gtt aat act cag act gtc act acc gtg aac gag att cag 739Gly Leu Ser Val Asn Thr Gln Thr Val Thr Thr Val Asn Glu Ile Gln
200 205 210cag gct gtt gaa gct ctc ggc gat gtt gat gtc atc tac gtt cca act 787Gln Ala Val Glu Ala Leu Gly Asp Val Asp Val Ile Tyr Val Pro Thr
215 220 225gac aac atg gtt gtt tcc ggt att tct tct ctg gtt cag gtt gct gag 835Asp Asn Met Val Val Ser Gly Ile Ser Ser Leu Val Gln Val Ala Glu230 235 240 245cag aag cag atc cct gtg atc ggc gct gag tcc ggc act gtt gag ggt 883Gln Lys Gln Ile Pro Val Ile Gly Ala Glu Ser Gly Thr Val Glu Gly
250 255 260ggc gca ctg gca acc ctg ggt atc gat tac acc gag ctt ggc cgc cag 931Gly Ala Leu Ala Thr Leu Gly Ile Asp Tyr Thr Glu Leu Gly Arg Gln
265 270 275act ggt gag atg gct ctg cgt att ctg cag gac ggc gaa gac cca gca 979Thr Gly Glu Met Ala Leu Arg Ile Leu Gln Asp Gly Glu Asp Pro Ala
280 285 290acc atg cct gtg gag act gca act gag ttc acc tac gtg atc aac gaa 1027Thr Met Pro Val Glu Thr Ala Thr Glu Phe Thr Tyr Val Ile Asn Glu
295 300 305gat gca gca gag cgc cag ggc gtg gag atc cct caa gag att ttg gat 1075Asp Ala Ala Glu Arg Gln Gly Val Glu Ile Pro Gln Glu Ile Leu Asp310 315 320 325aag gcc gaa cgc gta tgatcggcgc ttttgagttc gga 1113Lys Ala Glu Arg Val
330<210>504<211>330<212>PRT<213>谷氨酸棒杆菌<400>504Met Phe Ser Ser Arg Ser Lys Val Leu Ala Ser Ile Phe Thr Val Gly1 5 10 15Ala Leu Ala Leu Ala Ser Cys Ser Ser Asp Ser Ser Asp Ser Ser Thr
20 25 30Ser Thr Asp Ala Ala Gly Gly Asp Ser Tyr Arg Val Gly Ile Asn Gln
35 40 45Leu Val Gln His Pro Ala Leu Asp Ala Ala Thr Thr Gly Phe Lys Glu
50 55 60Ala Phe Glu Glu Ala Gly Val Asp Val Thr Phe Asp Glu Gln Asn Ala65 70 75 80Asn Gly Glu Gln Gly Thr Ala Leu Thr Ile Ser Gln Gln Phe Ala Ser
85 90 95Asp Asn Leu Asp Leu Val Leu Ala Val Ala Thr Pro Ala Ala Gln Ala
100 105 110Thr Ala Gln Asn Ile Thr Asp Ile Pro Val Leu Phe Thr Ala Val Thr
115 120 125Asp Ala Val Ser Ala Glu Leu Val Asp Ser Asn Glu Ala Pro Gly Gly
130 135 140Asn Val Thr Gly Thr Ser Asp Ile Ala Pro Ile Glu Gln Gln Leu Glu145 150 155 160Leu Leu Gln Gln Leu Val Pro Asp Ala Lys Ser Ile Gly Ile Val Tyr
165 170 175Ala Ser Gly Glu Val Asn Ser Gln Val Gln Val Asp Glu Val Thr Lys
180 185 190Ala Ala Glu Pro Leu Gly Leu Ser Val Asn Thr Gln Thr Val Thr Thr
195 200 205Val Asn Glu Ile Gln Gln Ala Val Glu Ala Leu Gly Asp Val Asp Val
210 215 220Ile Tyr Val Pro Thr Asp Asn Met Val Val Ser Gly Ile Ser Ser Leu225 230 235 240Val Gln Val Ala Glu Gln Lys Gln Ile Pro Val Ile Gly Ala Glu Ser
245 250 255Gly Thr Val Glu Gly Gly Ala Leu Ala Thr Leu Gly Ile Asp Tyr Thr
260 265 270Glu Leu Gly Arg Gln Thr Gly Glu Met Ala Leu Arg Ile Leu Gln Asp
275 280 285Gly Glu Asp Pro Ala Thr Met Pro Val Glu Thr Ala Thr Glu Phe Thr
290 295 300Tyr Val Ile Asn Glu Asp Ala Ala Glu Arg Gln Gly Val Glu Ile Pro305 310 315 320Gln Glu Ile Leu Asp Lys Ala Glu Arg Val
325 330<210>505<211>1263<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1240)<223>RXC00338<400>505tcttagaagg cgtagtcaca ccattaacct tgccagaatt tttcaaggct tggctagact 60tgggaaacga acatgcggta ccaaccaggg gagttaatgc gtg agt gat gta acc 115
Val Ser Asp Val Thr
1 5gtt ggc gat att cgc cgc att ttg gat gag gct tat ccg ccg gcg ttg 163Val Gly Asp Ile Arg Arg Ile Leu Asp Glu Ala Tyr Pro Pro Ala Leu
10 15 20gcg gaa agc tgg gac aaa gtg ggg ctg atc tgc ggt gat cca aca gag 211Ala Glu Ser Trp Asp Lys Val Gly Leu Ile Cys Gly Asp Pro Thr Glu
25 30 35tcg gtg aag cgt gtc ggt tta gca ctc gat tgc acc cag gca gtg gcc 259Ser Val Lys Arg Val Gly Leu Ala Leu Asp Cys Thr Gln Ala Val Ala
40 45 50gac aag gct gtg gac atg ggt ttg gac atg ctg atc att cac cac cca 307Asp Lys Ala Val Asp Met Gly Leu Asp Met Leu Ile Ile His His Pro
55 60 65ttg ctg ctg cgt ggg gtg acg tct gtt gct gcg gat gag cca aaa ggc 355Leu Leu Leu Arg Gly Val Thr Ser Val Ala Ala Asp Glu Pro Lys Gly70 75 80 85aag gtc att cac acc cta att cgc ggc ggg gtg gca ctg ttt tcc gcg 403Lys Val Ile His Thr Leu Ile Arg Gly Gly Val Ala Leu Phe Ser Ala
90 95 100cac act aat gcg gat tcc gcg cgc cca ggt gtc aac gat aaa ctc gcc 451His Thr Asn Ala Asp Ser Ala Arg Pro Gly Val Asn Asp Lys Leu Ala
105 110 115gag ctc gtc ggc atc acg gcc ggg cga ccc atc gcg aca cgg ctt tta 499Glu Leu Val Gly Ile Thr Ala Gly Arg Pro Ile Ala Thr Arg Leu Leu
120 125 130ggc ggc atg gac aaa tgg ggc gtg cac gtt ctg ccc aag gat gca gcg 547Gly Gly Met Asp Lys Trp Gly Val His Val Leu Pro Lys Asp Ala Ala
135 140 145tac cta aag aag atg ctt ttc gac gca ggt gcc ggt gcg atc ggc gac 595Tyr Leu Lys Lys Met Leu Phe Asp Ala Gly Ala Gly Ala Ile Gly Asp150 155 160 165tac cga gag tgt gcc ttt gag atc gaa gga acc ggg cag ttt agg ccc 643Tyr Arg Glu Cys Ala Phe Glu Ile Glu Gly Thr Gly Gln Phe Arg Pro
170 175 180gtg gag ggg gcg aat ccg gca gag ggg gac gtc gat aag ctt ttt aaa 691Val Glu Gly Ala Asn Pro Ala Glu Gly Asp Val Asp Lys Leu Phe Lys
185 190 195tcc ctt gag ctg cgc atc gag ttt gtt gca ccg cgc aac ctg cgc gcc 739Ser Leu Glu Leu Arg Ile Glu Phe Val Ala Pro Arg Asn Leu Arg Ala
200 205 210cgg ctc acg tcg gtg ctg cgg gag gct cat ccg tat gag gag cct gcc 787Arg Leu Thr Ser Val Leu Arg Glu Ala His Pro Tyr Glu Glu Pro Ala
215 220 225ttc gat att gtt gaa atg cac agc gct gag agt tta gaa aat gcg acc 835Phe Asp Ile Val Glu Met His Ser Ala Glu Ser Leu Glu Asn Ala Thr230 235 240 245gga ttg ggt cgt gtg ggt gaa ttg ccg gag ccg atg cgc ctc gcg gat 883Gly Leu Gly Arg Val Gly Glu Leu Pro Glu Pro Met Arg Leu Ala Asp
250 255 260ttc gtg caa caa gtg gcc aac aac ctg cct gtc acc gaa tgg ggc gtg 931Phe Val Gln Gln Val Ala Asn Asn Leu Pro Val Thr Glu Trp Gly Val
265 270 275cgc gct acc ggc gat cct gaa caa atg gtg tcc cgt gtg gcg gtt tca 979Arg Ala Thr Gly Asp Pro Glu Gln Met Val Ser Arg Val Ala Val Ser
280 285 290tca ggg tcg ggt gac agt ttc tta aac gat gtg att aag ctc gga gtg 1027Ser Gly Ser Gly Asp Ser Phe Leu Asn Asp Val Ile Lys Leu Gly Val
295 300 305gac gtt tat gtc act tct gat ctg cgc cac cat cca gtt gat gaa tat 1075Asp Val Tyr Val Thr Ser Asp Leu Arg His His Pro Val Asp Glu Tyr310 315 320 325ctc cga gaa ggt ggc cct gca gta atc gat act gca cac tgg gcc agc 1123Leu Arg Glu Gly Gly Pro Ala Val Ile Asp Thr Ala His Trp Ala Ser
330 335 340gaa ttt cca tgg act tcc caa gcc caa gaa att ttg cag gac aaa gcc 1171Glu Phe Pro Trp Thr Ser Gln Ala Gln Glu Ile Leu Gln Asp Lys Ala
345 350 355cca cag gtt gaa gtt gat gtg att tcg atc cgc aca gac ccc tgg acc 1219Pro Gln Val Glu Val Asp Val Ile Ser Ile Arg Thr Asp Pro Trp Thr
360 365 370atg tct gcg cga gca gtg aac taaattcttg agaactaaaa aag 1263Met Ser Ala Arg Ala Val Asn
375 380<210>506<211>380<212>PRT<213>谷氨酸棒杆菌<400>506Val Ser Asp Val Thr Val Gly Asp Ile Arg Arg Ile Leu Asp Glu Ala1 5 10 15Tyr Pro Pro Ala Leu Ala Glu Ser Trp Asp Lys Val Gly Leu Ile Cys
20 25 30Gly Asp Pro Thr Glu Ser Val Lys Arg Val Gly Leu Ala Leu Asp Cys
35 40 45Thr Gln Ala Val Ala Asp Lys Ala Val Asp Met Gly Leu Asp Met Leu
50 55 60Ile Ile His His Pro Leu Leu Leu Arg Gly Val Thr Ser Val Ala Ala65 70 75 80Asp Glu Pro Lys Gly Lys Val Ile His Thr Leu Ile Arg Gly Gly Val
85 90 95Ala Leu Phe Ser Ala His Thr Asn Ala Asp Ser Ala Arg Pro Gly Val
100 105 110Asn Asp Lys Leu Ala Glu Leu Val Gly Ile Thr Ala Gly Arg Pro Ile
115 120 125Ala Thr Arg Leu Leu Gly Gly Met Asp Lys Trp Gly Val His Val Leu
130 135 140Pro Lys Asp Ala Ala Tyr Leu Lys Lys Met Leu Phe Asp Ala Gly Ala145 150 155 160Gly Ala Ile Gly Asp Tyr Arg Glu Cys Ala Phe Glu Ile Glu Gly Thr
165 170 175Gly Gln Phe Arg Pro Val Glu Gly Ala Asn Pro Ala Glu Gly Asp Val
180 185 190Asp Lys Leu Phe Lys Ser Leu Glu Leu Arg Ile Glu Phe Val Ala Pro
195 200 205Arg Asn Leu Arg Ala Arg Leu Thr Ser Val Leu Arg Glu Ala His Pro
210 215 220Tyr Glu Glu Pro Ala Phe Asp Ile Val Glu Met His Ser Ala Glu Ser225 230 235 240Leu Glu Asn Ala Thr Gly Leu Gly Arg Val Gly Glu Leu Pro Glu Pro
245 250 255Met Arg Leu Ala Asp Phe Val Gln Gln Val Ala Asn Asn Leu Pro Val
260 265 270Thr Glu Trp Gly Val Arg Ala Thr Gly Asp Pro Glu Gln Met Val Ser
275 280 285Arg Val Ala Val Ser Ser Gly Ser Gly Asp Ser Phe Leu Asn Asp Val
290 295 300Ile Lys Leu Gly Val Asp Val Tyr Val Thr Ser Asp Leu Arg His His305 310 315 320Pro Val Asp Glu Tyr Leu Arg Glu Gly Gly Pro Ala Val Ile Asp Thr
325 330 335Ala His Trp Ala Ser Glu Phe Pro Trp Thr Ser Gln Ala Gln Glu Ile
340 345 350Leu Gln Asp Lys Ala Pro Gln Val Glu Val Asp Val Ile Ser Ile Arg
355 360 365Thr Asp Pro Trp Thr Met Ser Ala Arg Ala Val Asn
370 375 380<210>507<211>1470<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1447)<223>RXC00362<400>507cactttttgg gtgaaaattc cacgaagtta atgccgcttt aagtcaattc aatcacatgt 60aacatgctac ggttttttcg gtcacttaaa ggaggcgctt atg gga atc att gct 115
Met Gly Ile Ile Ala
1 5ctg ctc gtt ttt atc gca att gcc gtg ata ttg aat gtg ttt ttg aaa 163Leu Leu Val Phe Ile Ala Ile Ala Val Ile Leu Asn Val Phe Leu Lys
10 15 20cga gat att tca gaa gca ttg cta gtt gga tta gta gga act gcg ctt 211Arg Asp Ile Ser Glu Ala Leu Leu Val Gly Leu Val Gly Thr Ala Leu
25 30 35gtc ggc ggt gta aat gca ccg aca tta ctg att gat gct gta gtg gat 259Val Gly Gly Val Asn Ala Pro Thr Leu Leu Ile Asp Ala Val Val Asp
40 45 50gct gct cag tcg gaa gtt act ttc gca ggt atg gcc ttt gtt ttc atg 307Ala Ala Gln Ser Glu Val Thr Phe Ala Gly Met Ala Phe Val Phe Met
55 60 65ggc atc gtt gtg caa tca act gga ttg att gat cga tta atc gca atc 355Gly Ile Val Val Gln Ser Thr Gly Leu Ile Asp Arg Leu Ile Ala Ile70 75 80 85ctt aac tcg att ttt ggt cgg ctt cga ggt ggc gca ggt tat gtt tcc 403Leu Asn Ser Ile Phe Gly Arg Leu Arg Gly Gly Ala Gly Tyr Val Ser
90 95 100act ctt gga tct gcg ctc att gga ctc atc gct gga tca acg gct gga 451Thr Leu Gly Ser Ala Leu Ile Gly Leu Ile Ala Gly Ser Thr Ala Gly
105 110 115aac tcc gcg acg gtt ggc tca gtg acg atc cct tgg atg aaa aag acg 499Asn Ser Ala Thr Val Gly Ser Val Thr Ile Pro Trp Met Lys Lys Thr
120 125 130gga tgg act gct gaa agg tcc gca acg tta gtc gcg ggc aac tct ggc 547Gly Trp Thr Ala Glu Arg Ser Ala Thr Leu Val Ala Gly Asn Ser Gly
135 140 145ctt ggt gtt gcg ttg cct ccc aat tca aca atg ttc ate att ttg gca 595Leu Gly Val Ala Leu Pro Pro Asn Ser Thr Met Phe Ile Ile Leu Ala150 155 160 165ttg cca gct gca gca gct tct tcg gcc tct cag gtg tac att gct ttg 643Leu Pro Ala Ala Ala Ala Ser Ser Ala Ser Gln Val Tyr Ile Ala Leu
170 175 180gct tgt ggt ggt gcg tat gca gtg ctc tac cgc tta gcg gtc gtc ttt 691Ala Cys Gly Gly Ala Tyr Ala Val Leu Tyr Arg Leu Ala Val Val Phe
185 190 195tac tgg aca cgt aaa gat aaa att cct gcc acc cct gat gat caa cgg 739Tyr Trp Thr Arg Lys Asp Lys Ile Pro Ala Thr Pro Asp Asp Gln Arg
200 205 210gtg tca ttc ggt gag gca atg aag act gga tgg cgt tca ccg ttg atc 787Val Ser Phe Gly Glu Ala Met Lys Thr Gly Trp Arg Ser Pro Leu Ile
215 220 225ttc ctt gga att ttg atc ccc gta atc ctc aca atc ggc cca ttg tct 835Phe Leu Gly Ile Leu Ile Pro Val Ile Leu Thr Ile Gly Pro Leu Ser230 235 240 245gaa tgg tta aag aca cat gga gtt ggg gag tct ggt gtt aaa tcg atg 883Glu Trp Leu Lys Thr His Gly Val Gly Glu Ser Gly Val Lys Ser Met
250 255 260tcg atc atc gtg tgg gtg cca att ctg att acg gca att gct ctg att 931Ser Ile Ile Val Trp Val Pro Ile Leu Ile Thr Ala Ile Ala Leu Ile
265 270 275gaa ggg cgt aaa cga att gct aac aac atg gca cac ttt agg gtt cag 979Glu Gly Arg Lys Arg Ile Ala Asn Asn Met Ala His Phe Arg Val Gln
280 285 290atc tcc aag gac ttg cca caa ttt gcc acc gta gga att tcg ttg ttt 1027Ile Ser Lys Asp Leu Pro Gln Phe Ala Thr Val Gly Ile Ser Leu Phe
295 300 305tct gcg ctt gca gca gcg aac atc atg gaa gaa ctg ggt gtt ggc ccg 1075Ser Ala Leu Ala Ala Ala Asn Ile Met Glu Glu Leu Gly Val Gly Pro310 315 320 325cag ttg tct aac tgg ctt gat tcc atg gac cta cct aag tct gtc atg 1123Gln Leu Ser Asn Trp Leu Asp Ser Met Asp Leu Pro Lys Ser Val Met
330 335 340gtg atc att gtc tgc atc atg tgc att gtg gtg gca acg cca ctg tcg 1171Val Ile Ile Val Cys Ile Met Cys Ile Val Val Ala Thr Pro Leu Ser
345 350 355tca aca gca acc gcg gct gcg att ggt gct ccc gct gtc gct gcg ttg 1219Ser Thr Ala Thr Ala Ala Ala Ile Gly Ala Pro Ala Val Ala Ala Leu
360 365 370gct gcg gta ggt att gat cca act gtg gcg atc gta gtg atc ttg ctg 1267Ala Ala Val Gly Ile Asp Pro Thr Val Ala Ile Val Val Ile Leu Leu
375 380 385tgc act tcc act gaa ggt gca tcc ccg ccg gtg ggc gcg ccg att tac 1315Cys Thr Ser Thr Glu Gly Ala Ser Pro Pro Val Gly Ala Pro Ile Tyr390 395 400 405ctt tct gct gcg atc gcc gat gca aac cca acg aaa atg ttc gta cca 1363Leu Ser Ala Ala Ile Ala Asp Ala Asn Pro Thr Lys Met Phe Val Pro
410 415 420ctg att acg tac ttt gtt gtc ccc atg att ctg ctt gct tgg cta gtt 1411Leu Ile Thr Tyr Phe Val Val Pro Met Ile Leu Leu Ala Trp Leu Val
425 430 435gga atg gga ttc tta cca gtg att gtt cct acg ggt taaaggggta 1457Gly Met Gly Phe Leu Pro Val Ile Val Pro Thr Gly
440 445aaaatgaact caa 1470<210>508<211>449<212>PRT<213>谷氨酸棒杆菌<400>508Met Gly Ile Ile Ala Leu Leu Val Phe Ile Ala Ile Ala Val Ile Leu1 5 10 15Asn Val Phe Leu Lys Arg Asp Ile Ser Glu Ala Leu Leu Val Gly Leu
20 25 30Val Gly Thr Ala Leu Val Gly Gly Val Asn Ala Pro Thr Leu Leu Ile
35 40 45Asp Ala Val Val Asp Ala Ala Gln Ser Glu Val Thr Phe Ala Gly Met
50 55 60Ala Phe Val Phe Met Gly Ile Val Val Gln Ser Thr Gly Leu Ile Asp65 70 75 80Arg Leu Ile Ala Ile Leu Asn Ser Ile Phe Gly Arg Leu Arg Gly Gly
85 90 95Ala Gly Tyr Val Ser Thr Leu Gly Ser Ala Leu Ile Gly Leu Ile Ala
100 105 110Gly Ser Thr Ala Gly Asn Ser Ala Thr Val Gly Ser Val Thr Ile Pro
115 120 125Trp Met Lys Lys Thr Gly Trp Thr Ala Glu Arg Ser Ala Thr Leu Val
130 135 140Ala Gly Asn Ser Gly Leu Gly Val Ala Leu Pro Pro Asn Ser Thr Met145 150 155 160Phe Ile Ile Leu Ala Leu Pro Ala Ala Ala Ala Ser Ser Ala Ser Gln
165 170 175Val Tyr Ile Ala Leu Ala Cys Gly Gly Ala Tyr Ala Val Leu Tyr Arg
180 185 190Leu Ala Val Val Phe Tyr Trp Thr Arg Lys Asp Lys Ile Pro Ala Thr
195 200 205Pro Asp Asp Gln Arg Val Ser Phe Gly Glu Ala Met Lys Thr Gly Trp
210 215 220Arg Ser Pro Leu Ile Phe Leu Gly Ile Leu Ile Pro Val Ile Leu Thr225 230 235 240Ile Gly Pro Leu Ser Glu Trp Leu Lys Thr His Gly Val Gly Glu Ser
245 250 255Gly Val Lys Ser Met Ser Ile Ile Val Trp Val Pro Ile Leu Ile Thr
260 265 270Ala Ile Ala Leu Ile Glu Gly Arg Lys Arg Ile Ala Asn Asn Met Ala
275 280 285His Phe Arg Val Gln Ile Ser Lys Asp Leu Pro Gln Phe Ala Thr Val
290 295 300Gly Ile Ser Leu Phe Ser Ala Leu Ala Ala Ala Asn Ile Met Glu Glu305 310 315 320Leu Gly Val Gly Pro Gln Leu Ser Asn Trp Leu Asp Ser Met Asp Leu
325 330 335Pro Lys Ser Val Met Val Ile Ile Val Cys Ile Met Cys Ile Val Val
340 345 350Ala Thr Pro Leu Ser Ser Thr Ala Thr Ala Ala Ala Ile Gly Ala Pro
355 360 365Ala Val Ala Ala Leu Ala Ala Val Gly Ile Asp Pro Thr Val Ala Ile
370 375 380Val Val Ile Leu Leu Cys Thr Ser Thr Glu Gly Ala Ser Pro Pro Val385 390 395 400Gly Ala Pro Ile Tyr Leu Ser Ala Ala Ile Ala Asp Ala Asn Pro Thr
405 410 415Lys Met Phe Val Pro Leu Ile Thr Tyr Phe Val Val Pro Met Ile Leu
420 425 430Leu Ala Trp Leu Val Gly Met Gly Phe Leu Pro Val Ile Val Pro Thr
435 440 445Gly<210>509<211>1203<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1180)<223>RXC00412<400>509cttttgacga acaccacgtc gcgtacgctt cctcggggcg ttaaactatt tgtcttccag 60cttttgtccc ccgacttttg tacgaatcga ggacaccgtc gtg tca cac acc gcg 115
Val Ser His Thr Ala
1 5tcc aca ccg acg cca gag gaa tac tcc gcg cag caa ccc agc acc cag 163Ser Thr Pro Thr Pro Glu Glu Tyr Ser Ala Gln Gln Pro Ser Thr Gln
10 15 20ggc act cgc gtt gag ttc cgc ggc ata acc aaa gtc ttt agc aac aat 211Gly Thr Arg Val Glu Phe Arg Gly Ile Thr Lys Val Phe Ser Asn Asn
25 30 35aaa tct gct aaa acc acc gcg ctt gat aat gtc act ctc acc gta gaa 259Lys Ser Ala Lys Thr Thr Ala Leu Asp Asn Val Thr Leu Thr Val Glu
40 45 50ccc ggt gag gta atc ggc atc atc ggt tac tct ggc gcc ggc aag tcc 307Pro Gly Glu Val Ile Gly Ile Ile Gly Tyr Ser Gly Ala Gly Lys Ser
55 60 65act ctt gtc cgc ctc atc aat ggc ctt gac tcc ccc acg agc ggt tcg 355Thr Leu Val Arg Leu Ile Asn Gly Leu Asp Ser Pro Thr Ser Gly Ser70 75 80 85ttg ctg ctc aac ggc acc gac atc gtc gga atg ccc gag tct aag ctg 403Leu Leu Leu Asn Gly Thr Asp Ile Val Gly Met Pro Glu Ser Lys Leu
90 95 100cgt aaa ctg cgc agt aat atc ggc atg att ttc cag cag ttc aac ctg 451Arg Lys Leu Arg Ser Asn Ile Gly Met Ile Phe Gln Gln Phe Asn Leu
105 110 115ttc cag tcg cgt act gcg gct gga aat gtg gag tac ccg ctg gaa gtt 499Phe Gln Ser Arg Thr Ala Ala Gly Asn Val Glu Tyr Pro Leu Glu Val
120 125 130gcc aag atg gac aag gca gct cgt aaa gct cgc gtg caa gaa atg ctc 547Ala Lys Met Asp Lys Ala Ala Arg Lys Ala Arg Val Gln Glu Met Leu
135 140 145gag ttc gtc ggc ctg ggc gac aaa ggc aaa aac tac ccc gag cag ctg 595Glu Phe Val Gly Leu Gly Asp Lys Gly Lys Asn Tyr Pro Glu Gln Leu150 155 160 165tcg ggc ggc cag aag cag cgc gtc ggc att gcc cgt gca ctg gcc acc 643Ser Gly Gly Gln Lys Gln Arg Val Gly Ile Ala Arg Ala Leu Ala Thr
170 175 180aat cca acg ctt ttg ctt gcc gac gaa gcc acc tcc gct ttg gac cca 691Asn Pro Thr Leu Leu Leu Ala Asp Glu Ala Thr Ser Ala Leu Asp Pro
185 190 195gaa acc acc cat gaa gtt ctg gag ctg ctg cgc aag gta aac cgc gaa 739Glu Thr Thr His Glu Val Leu Glu Leu Leu Arg Lys Val Asn Arg Glu
200 205 210ctg ggc atc acc atc gtt gtg atc acc cac gaa atg gaa gtt gtg cgt 787Leu Gly Ile Thr Ile Val Val Ile Thr His Glu Met Glu Val Val Arg
215 220 225tcc atc gca gac aag gtt gct gtg atg gaa tcc ggc aaa gtt gtg gaa 835Ser Ile Ala Asp Lys Val Ala Val Met Glu Ser Gly Lys Val Val Glu230 235 240 245tac ggc agc gtc tac gag gtg ttc tcc aat cca caa aca cag gtt gct 883Tyr Gly Ser Val Tyr Glu Val Phe Ser Asn Pro Gln Thr Gln Val Ala
250 255 260caa aag ttc gtg gcc acc gcg ctg cgt aac acc cca gac caa gtg gaa 931Gln Lys Phe Val Ala Thr Ala Leu Arg Asn Thr Pro Asp Gln Val Glu
265 270 275tcg gaa gat ctg ctt agc cat gag gga cgt ctg ttc acc att gat ctg 979Ser Glu Asp Leu Leu Ser His Glu Gly Arg Leu Phe Thr Ile Asp Leu
280 285 290act gaa acg tcc ggc ttc ttt gca gca acc gct cgt gct gcc gaa caa 1027Thr Glu Thr Ser Gly Phe Phe Ala Ala Thr Ala Arg Ala Ala Glu Gln
295 300 305ggt gct ttt gtc aac atc gtt cac ggt ggc gtg acc acc ttg caa cgc 1075Gly Ala Phe Val Asn Ile Val His Gly Gly Val Thr Thr Leu Gln Arg310 315 320 325caa tca ttt ggc aaa atg act gtt cga ctc acc ggc aac acc gct gcg 1123Gln Ser Phe Gly Lys Met Thr Val Arg Leu Thr Gly Asn Thr Ala Ala
330 335 340att gaa gag ttc tat caa acc ttg acc aag acc acg acc atc aag gag 1171Ile Glu Glu Phe Tyr Gln Thr Leu Thr Lys Thr Thr Thr Ile Lys Glu
345 350 355atc acc cga tgaacgagat gatcctcgca gct 1203Ile Thr Arg
360<210>510<211>360<212>PRT<213>谷氨酸棒杆菌<400>510Val Ser His Thr Ala Ser Thr Pro Thr Pro Glu Glu Tyr Ser Ala Gln1 5 10 15Gln Pro Ser Thr Gln Gly Thr Arg Val Glu Phe Arg Gly Ile Thr Lys
20 25 30Val Phe Ser Asn Asn Lys Ser Ala Lys Thr Thr Ala Leu Asp Asn Val
35 40 45Thr Leu Thr Val Glu Pro Gly Glu Val Ile Gly Ile Ile Gly Tyr Ser
50 55 60Gly Ala Gly Lys Ser Thr Leu Val Arg Leu Ile Asn Gly Leu Asp Ser65 70 75 80Pro Thr Ser Gly Ser Leu Leu Leu Asn Gly Thr Asp Ile Val Gly Met
85 90 95Pro Glu Ser Lys Leu Arg Lys Leu Arg Ser Asn Ile Gly Met Ile Phe
100 105 110Gln Gln Phe Asn Leu Phe Gln Ser Arg Thr Ala Ala Gly Asn Val Glu
115 120 125Tyr Pro Leu Glu Val Ala Lys Met Asp Lys Ala Ala Arg Lys Ala Arg
130 135 140Val Gln Glu Met Leu Glu Phe Val Gly Leu Gly Asp Lys Gly Lys Asn145 150 155 160Tyr Pro Glu Gln Leu Ser Gly Gly Gln Lys Gln Arg Val Gly Ile Ala
165 170 175Arg Ala Leu Ala Thr Asn Pro Thr Leu Leu Leu Ala Asp Glu Ala Thr
180 185 190Ser Ala Leu Asp Pro Glu Thr Thr His Glu Val Leu Glu Leu Leu Arg
195 200 205Lys Val Asn Arg Glu Leu Gly Ile Thr Ile Val Val Ile Thr His Glu
210 215 220Met Glu Val Val Arg Ser Ile Ala Asp Lys Val Ala Val Met Glu Ser225 230 235 240Gly Lys Val Val Glu Tyr Gly Ser Val Tyr Glu Val Phe Ser Asn Pro
245 250 255Gln Thr Gln Val Ala Gln Lys Phe Val Ala Thr Ala Leu Arg Asn Thr
260 265 270Pro Asp Gln Val Glu Ser Glu Asp Leu Leu Ser His Glu Gly Arg Leu
275 280 285Phe Thr Ile Asp Leu Thr Glu Thr Ser Gly Phe Phe Ala Ala Thr Ala
290 295 300Arg Ala Ala Glu Gln Gly Ala Phe Val Asn Ile Val His Gly Gly Val305 310 315 320Thr Thr Leu Gln Arg Gln Ser Phe Gly Lys Met Thr Val Arg Leu Thr
325 330 335Gly Asn Thr Ala Ala Ile Glu Glu Phe Tyr Gln Thr Leu Thr Lys Thr
340 345 350Thr Thr Ile Lys Glu Ile Thr Arg
355 360<210>511<211>813<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(790)<223>RXC00526<400>511ggtggagcag gcggcggctc cttttagtcc tgcggcccct tttgaccctg cagcccctgc 60cgtttctgcc aagcaaaccg tgggccaggt gatttagcct atg agc ctc atc gaa 115
Met Ser Leu Ile Glu
1 5atg cga aat att gtc aag acc tac aac att gga tct gaa ggt gaa ctc 163Met Arg Asn Ile Val Lys Thr Tyr Asn Ile Gly Ser Glu Gly Glu Leu
10 15 20acc gtg ttg cac ggt gtg gat ttc cat gtg gac cgt ggc gaa ttc gtg 211Thr Val Leu His Gly Val Asp Phe His Val Asp Arg Gly Glu Phe Val
25 30 35tcg gtt gtg ggt acg tcc ggc tca ggt aaa tca acg atg atg aac atc 259Ser Val Val Gly Thr Ser Gly Ser Gly Lys Ser Thr Met Met Asn Ile
40 45 50att ggg ttg ttg gat aag cca act gat ggc acg tac acc ttg gat ggc 307Ile Gly Leu Leu Asp Lys Pro Thr Asp Gly Thr Tyr Thr Leu Asp Gly
55 60 65gtg gat gtg ttg gat atc agc gat gat gct ttg gcg agc cac cgc gct 355Val Asp Val Leu Asp Ile Ser Asp Asp Ala Leu Ala Ser His Arg Ala70 75 80 85aaa tcg att ggt ttt gtg ttt cag aac ttc aat ctg att ggc cgg atc 403Lys Ser Ile Gly Phe Val Phe Gln Asn Phe Asn Leu Ile Gly Arg Ile
90 95 100gat gcg ttg aag aat gtg gaa atg ccc atg atg tat gcg ggc att ccg 451Asp Ala Leu Lys Asn Val Glu Met Pro Met Met Tyr Ala Gly Ile Pro
105 110 115gct aag cag cgg aga agt cgt gcg gtt gaa tta ttg gaa atg gtc ggg 499Ala Lys Gln Arg Arg Ser Arg Ala Val Glu Leu Leu Glu Met Val Gly
120 125 130atg ggt gag cgt ctc aac cat gag ccc aat gag ctt tcg ggt ggt cag 547Met Gly Glu Arg Leu Asn His Glu Pro Asn Glu Leu Ser Gly Gly Gln
135 140 145aag cag cgc gtg gcc att gct cgc gcg ttg gcg aac gat cct gag atc 595Lys Gln Arg Val Ala Ile Ala Arg Ala Leu Ala Asn Asp Pro Glu Ile150 155 160 165att ctt gct gat gaa cca act ggt gcg ttg gat tct gca acg ggc cgg 643Ile Leu Ala Asp Glu Pro Thr Gly Ala Leu Asp Ser Ala Thr Gly Arg
170 175 180atg gtg atg gat att ttc cac cag ctc aac aag gag cag ggc aaa acc 691Met Val Met Asp Ile Phe His Gln Leu Asn Lys Glu Gln Gly Lys Thr
185 190 195atc gtg ttt att act cac aac cct gag ctt gct gat gaa tct gat cgg 739Ile Val Phe Ile Thr His Asn Pro Glu Leu Ala Asp Glu Ser Asp Arg
200 205 210gtg gtc acc atg gtt gac ggg cgc atc att ggg tct gag gtg aaa cac 787Val Val Thr Met Val Asp Gly Arg Ile Ile Gly Ser Glu Val Lys His
215 220 225tca tgagccttgc agaatcaatt ctt 813Ser230<210>512<211>230<212>PRT<213>谷氨酸棒杆菌<400>512Met Ser Leu Ile Glu Met Arg Asn Ile Val Lys Thr Tyr Asn Ile Gly1 5 10 15Ser Glu Gly Glu Leu Thr Val Leu His Gly Val Asp Phe His Val Asp
20 25 30Arg Gly Glu Phe Val Ser Val Val Gly Thr Ser Gly Ser Gly Lys Ser
35 40 45Thr Met Met Asn Ile Ile Gly Leu Leu Asp Lys Pro Thr Asp Gly Thr
50 55 60Tyr Thr Leu Asp Gly Val Asp Val Leu Asp Ile Ser Asp Asp Ala Leu65 70 75 80Ala Ser His Arg Ala Lys Ser Ile Gly Phe Val Phe Gln Asn Phe Asn
85 90 95Leu Ile Gly Arg Ile Asp Ala Leu Lys Asn Val Glu Met Pro Met Met
100 105 110Tyr Ala Gly Ile Pro Ala Lys Gln Arg Arg Ser Arg Ala Val Glu Leu
115 120 125Leu Glu Met Val Gly Met Gly Glu Arg Leu Asn His Glu Pro Asn Glu
130 135 140Leu Ser Gly Gly Gln Lys Gln Arg Val Ala Ile Ala Arg Ala Leu Ala145 150 155 160Asn Asp Pro Glu Ile Ile Leu Ala Asp Glu Pro Thr Gly Ala Leu Asp
165 170 175Ser Ala Thr Gly Arg Met Val Met Asp Ile Phe His Gln Leu Asn Lys
180 185 190Glu Gln Gly Lys Thr Ile Val Phe Ile Thr His Asn Pro Glu Leu Ala
195 200 205Asp Glu Ser Asp Arg Val Val Thr Met Val Asp Gly Arg Ile Ile Gly
210 215 220Ser Glu Val Lys His Ser225 230<210>513<211>1185<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1162)<223>RXC01004<400>513ccggacgctg gatcgcacga gtaacggtca tggaagatcg acgcatcgac aaagccgttc 60tcacccccat cacccatgaa gaagcaaagg agtacgaaaa gtg agt att tgg gca 115
Val Ser Ile Trp Ala
1 5act gtc ctt cta att atc gtc ctt ctt tcc gcc aac gcc ttc ttc gtg 163Thr Val Leu Leu Ile Ile Val Leu Leu Ser Ala Asn Ala Phe Phe Val
10 15 20gcc gcg gag ttc gca ctg att tcc tcg cgc cgg gac cgc ctg gat tcc 211Ala Ala Glu Phe Ala Leu Ile Ser Ser Arg Arg Asp Arg Leu Asp Ser
25 30 35ctg gta tcc cag ggt aaa aag gga gct gaa aag gtt ctc tac gca acc 259Leu Val Ser Gln Gly Lys Lys Gly Ala Glu Lys Val Leu Tyr Ala Thr
40 45 50gag cac ctc tcc atc atg ttg gcg ggc gct cag ttc ggt att acg gtc 307Glu His Leu Ser Ile Met Leu Ala Gly Ala Gln Phe Gly Ile Thr Val
55 60 65tgt tct ctg att ctg ggt aaa gtc gca gaa cct gcg atc gcc cac ttc 355Cys Ser Leu Ile Leu Gly Lys Val Ala Glu Pro Ala Ile Ala His Phe70 75 80 85att gag gtg cct ttc acc tcc tgg ggt gtt cca aat gat ttg atc cac 403Ile Glu Val Pro Phe Thr Ser Trp Gly Val Pro Asn Asp Leu Ile His
90 95 100cca att tcc ttc gtc atc gca ctg gcg atc atc acc tgg ttg cac att 451Pro Ile Ser Phe Val Ile Ala Leu Ala Ile Ile Thr Trp Leu His Ile
105 110 115ctc ttt ggt gaa atg gtg cca aag aac atc gct att gct ggc cct gaa 499Leu Phe Gly Glu Met Val Pro Lys Asn Ile Ala Ile Ala Gly Pro Glu
120 125 130acc tta ggc atg tgg ctt gct cca gtg ctc att gcg ttt gtg aag att 547Thr Leu Gly Met Trp Leu Ala Pro Val Leu Ile Ala Phe Val Lys Ile
135 140 145acc cgc ccg ttg atc gag ttc atg aac tgg atc gcc cgt ctg acc ctt 595Thr Arg Pro Leu Ile Glu Phe Met Asn Trp Ile Ala Arg Leu Thr Leu150 155 160 165cgc gcc ttt ggt gtg gag caa aaa aac gag ctg gat tcc acc gtg gac 643Arg Ala Phe Gly Val Glu Gln Lys Asn Glu Leu Asp Ser Thr Val Asp
170 175 180cca gag cag ctg gca tca atg att tcc gag tcc cgt tcc gaa ggc ctc 691Pro Glu Gln Leu Ala Ser Met Ile Ser Glu Ser Arg Ser Glu Gly Leu
185 190 195ctt gat gct gaa gag cac gcc cgc ctg tcc aag gcg ctg cgc tct gag 739Leu Asp Ala Glu Glu His Ala Arg Leu Ser Lys Ala Leu Arg Ser Glu
200 205 210cag cgt tcc atc aag gaa ctg gtg att aag gat gag gac gtg cgc acg 787Gln Arg Ser Ile Lys Glu Leu Val Ile Lys Asp Glu Asp Val Arg Thr
215 220 225ctg gcg ttc ggt aaa tct ggc ccg acc ttg cac cag ttg gag gaa gca 835Leu Ala Phe Gly Lys Ser Gly Pro Thr Leu His Gln Leu Glu Glu Ala230 235 240 245gtc cgc gag acc ggt ttc tcc cgc ttc cct gtc acc ggc cgc gat gga 883Val Arg Glu Thr Gly Phe Ser Arg Phe Pro Val Thr Gly Arg Asp Gly
250 255 260tcc tac ttg ggt tat atc cac atc aag gat att ttg cct cgt ctg gct 931Ser Tyr Leu Gly Tyr Ile His Ile Lys Asp Ile Leu Pro Arg Leu Ala
265 270 275gat cct gag atg gat ccc tcc gag acc att ccg cgt tct gca ctg cgc 979Asp Pro Glu Met Asp Pro Ser Glu Thr Ile Pro Arg Ser Ala Leu Arg
280 285 290cct ttg agc aat gtg gat gcc gac ggc ctc atg gat gac gtc ttg gat 1027Pro Leu Ser Asn Val Asp Ala Asp Gly Leu Met Asp Asp Val Leu Asp
295 300 305ttt atg cac tac cgc tcc gcg cac atg gct cag gtt cgc ctc aaa ggt 1075Phe Met His Tyr Arg Ser Ala His Met Ala Gln Val Arg Leu Lys Gly310 315 320 325gag ctt ctc ggc gtg att acg ctg gag gat ctc atc gaa gaa tac gtg 1123Glu Leu Leu Gly Val Ile Thr Leu Glu Asp Leu Ile Glu Glu Tyr Val
330 335 340ggc acc gtc aac gat tgg act cac gaa agc tcc gac gac tagaaatagt 1172Gly Thr Val Asn Asp Trp Thr His Glu Ser Ser Asp Asp
345 350aactgtgttg gac 1185<210>514<211>354<212>PRT<213>谷氨酸棒杆菌<400>514Val Ser Ile Trp Ala Thr Val Leu Leu Ile Ile Val Leu Leu Ser Ala1 5 10 15Asn Ala Phe Phe Val Ala Ala Glu Phe Ala Leu Ile Ser Ser Arg Arg
20 25 30Asp Arg Leu Asp Ser Leu Val Ser Gln Gly Lys Lys Gly Ala Glu Lys
35 40 45Val Leu Tyr Ala Thr Glu His Leu Ser Ile Met Leu Ala Gly Ala Gln
50 55 60Phe Gly Ile Thr Val Cys Ser Leu Ile Leu Gly Lys Val Ala Glu Pro65 70 75 80Ala Ile Ala His Phe Ile Glu Val Pro Phe Thr Ser Trp Gly Val Pro
85 90 95Asn Asp Leu Ile His Pro Ile Ser Phe Val Ile Ala Leu Ala Ile Ile
100 105 110Thr Trp Leu His Ile Leu Phe Gly Glu Met Val Pro Lys Asn Ile Ala
115 120 125Ile Ala Gly Pro Glu Thr Leu Gly Met Trp Leu Ala Pro Val Leu Ile
130 135 140Ala Phe Val Lys Ile Thr Arg Pro Leu Ile Glu Phe Met Asn Trp Ile145 150 155 160Ala Arg Leu Thr Leu Arg Ala Phe Gly Val Glu Gln Lys Asn Glu Leu
165 170 175Asp Ser Thr Val Asp Pro Glu Gln Leu Ala Ser Met Ile Ser Glu Ser
180 185 190Arg Ser Glu Gly Leu Leu Asp Ala Glu Glu His Ala Arg Leu Ser Lys
195 200 205Ala Leu Arg Ser Glu Gln Arg Ser Ile Lys Glu Leu Val Ile Lys Asp
210 215 220Glu Asp Val Arg Thr Leu Ala Phe Gly Lys Ser Gly Pro Thr Leu His225 230 235 240Gln Leu Glu Glu Ala Val Arg Glu Thr Gly Phe Ser Arg Phe Pro Val
245 250 255Thr Gly Arg Asp Gly Ser Tyr Leu Gly Tyr Ile His Ile Lys Asp Ile
260 265 270Leu Pro Arg Leu Ala Asp Pro Glu Met Asp Pro Ser Glu Thr Ile Pro
275 280 285Arg Ser Ala Leu Arg Pro Leu Ser Asn Val Asp Ala Asp Gly Leu Met
290 295 300Asp Asp Val Leu Asp Phe Met His Tyr Arg Ser Ala His Met Ala Gln305 310 315 320Val Arg Leu Lys Gly Glu Leu Leu Gly Val Ile Thr Leu Glu Asp Leu
325 330 335Ile Glu Glu Tyr Val Gly Thr Val Asn Asp Trp Thr His Glu Ser Ser
340 345 350Asp Asp<210>515<211>732<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(709)<223>RXC01017<400>515gaaatttgag ggggcgctac ccttagaagg tgcgcaatga caccacgata gttcgcgcct 60agtgtggatt gctagaaaac tttaagaaag aggaaataat atg gct caa aaa gta 115
Met Ala Gln Lys Val
1 5acc ttc tgg ttc gat acc acc tgc cca ttc tgc tgg gtc acc tcc cgc 163Thr Phe Trp Phe Asp Thr Thr Cys Pro Phe Cys Trp Val Thr Ser Arg
10 15 20tgg att aag gaa gtc gaa caa gtc cgc gat att gaa atc cag tgg gtt 211Trp Ile Lys Glu Val Glu Gln Val Arg Asp Ile Glu Ile Gln Trp Val
25 30 35cca atg agc ctc gct gtc cta aac gaa ggc cgt gat ctc cca gag gat 259Pro Met Ser Leu Ala Val Leu Asn Glu Gly Arg Asp Leu Pro Glu Asp
40 45 50tac aag gag cgc atg aag gct gca tgg gga cca gca cgc gtt ttc gca 307Tyr Lys Glu Arg Met Lys Ala Ala Trp Gly Pro Ala Arg Val Phe Ala
55 60 65gct gtc gcc acc gac cat gct gac aag ctc ggc gac ctg tac acc gca 355Ala Val Ala Thr Asp His Ala Asp Lys Leu Gly Asp Leu Tyr Thr Ala70 75 80 85atg ggt acc cgc atc cac aac gac ggt cgc gga cca atc gaa ggt tcc 403Met Gly Thr Arg Ile His Asn Asp Gly Arg Gly Pro Ile Glu Gly Ser
90 95 100ttc aat gat gtc atc gca gag gca ctt gaa gag gtc ggc cta gac gct 451Phe Asn Asp Val Ile Ala Glu Ala Leu Glu Glu Val Gly Leu Asp Ala
105 110 115gca ctt ggt gaa gtt gca gac acc acc gaa tgg gac gac gca ctt cgc 499Ala Leu Gly Glu Val Ala Asp Thr Thr Glu Trp Asp Asp Ala Leu Arg
120 125 130gca ttc cac cag acc gca atg gac gag gtc ggc aac gat gtc gga acc 547Ala Phe His Gln Thr Ala Met Asp Glu Val Gly Asn Asp Val Gly Thr
135 140 145cca gtg gtc aag ctc ggc gac acc gct ttc ttc ggc cca gtg ctc acc 595Pro Val Val Lys Leu Gly Asp Thr Ala Phe Phe Gly Pro Val Leu Thr150 155 160 165cgc atc cca cgc ggc gag gaa gca gga gag atc ttc gac gct tcc ttc 643Arg Ile Pro Arg Gly Glu Glu Ala Gly Glu Ile Phe Asp Ala Ser Phe
170 175 180aag ctc gca agc tat ccc cac ttc ttt gaa atc aag cgc agc cgc act 691Lys Leu Ala Ser Tyr Pro His Phe Phe Glu Ile Lys Arg Ser Arg Thr
185 190 195gag aac cca cag ttc gac taattaacgc tgtctctgct tat 732Glu Asn Pro Gln Phe Asp
200<210>516<21l>203<212>PRT<213>谷氨酸棒杆菌<400>516Met Ala Gln Lys Val Thr Phe Trp Phe Asp Thr Thr Cys Pro Phe Cys1 5 10 15Trp Val Thr Ser Arg Trp Ile Lys Glu Val Glu Gln Val Arg Asp Ile
20 25 30Glu Ile Gln Trp Val Pro Met Ser Leu Ala Val Leu Asn Glu Gly Arg
35 40 45Asp Leu Pro Glu Asp Tyr Lys Glu Arg Met Lys Ala Ala Trp Gly Pro
50 55 60Ala Arg Val Phe Ala Ala Val Ala Thr Asp His Ala Asp Lys Leu Gly65 70 75 80Asp Leu Tyr Thr Ala Met Gly Thr Arg Ile His Asn Asp Gly Arg Gly
85 90 95Pro Ile Glu Gly Ser Phe Asn Asp Val Ile Ala Glu Ala Leu Glu Glu
100 105 110Val Gly Leu Asp Ala Ala Leu Gly Glu Val Ala Asp Thr Thr Glu Trp
115 120 125Asp Asp Ala Leu Arg Ala Phe His Gln Thr Ala Met Asp Glu Val Gly
130 135 140Asn Asp Val Gly Thr Pro Val Val Lys Leu Gly Asp Thr Ala Phe Phe145 150 155 160Gly Pro Val Leu Thr Arg Ile Pro Arg Gly Glu Glu Ala Gly Glu Ile
165 170 175Phe Asp Ala Ser Phe Lys Leu Ala Ser Tyr Pro His Phe Phe Glu Ile
180 185 190Lys Arg Ser Arg Thr Glu Asn Pro Gln Phe Asp
195 200<210>517<211>622<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(622)<223>RXC01021<400>517cgagaggctt ttttggctct aagcctttta gtcgtgcgaa cgaaatctta agcagcctcg 60gtgccaccga gatcgattgg tcgctgtaag gtatctgatt atg tcc agt tcc gaa 115
Met Ser Ser Ser Glu
1 5agc tcg cgt tcc gaa ggc tcg cag cca gca ccg tct gta cag cct gaa 163Ser Ser Arg Ser Glu Gly Ser Gln Pro Ala Pro Ser Val Gln Pro Glu
10 15 20cgc cgt gct gat tca acg ggg gct cct gcg gca gct tcc aag gaa gct 211Arg Arg Ala Asp Ser Thr Gly Ala Pro Ala Ala Ala Ser Lys Glu Ala
25 30 35tcc caa caa atg gac gct gcc gga gtt ctt gag tgg gcc agg acc gct 259Ser Gln Gln Met Asp Ala Ala Gly Val Leu Glu Trp Ala Arg Thr Ala
40 45 50gtc gag cag ctt tct gaa cgt cgt gca gag atc aat gca ctg aat gtc 307Val Glu Gln Leu Ser Glu Arg Arg Ala Glu Ile Asn Ala Leu Asn Val
55 60 65ttt cct gtt cca gat gca gac act gga tca aac atg acc tac acc atg 355Phe Pro Val Pro Asp Ala Asp Thr Gly Ser Asn Met Thr Tyr Thr Met70 75 80 85aca gct gcg ttg gat gaa gcg ctg aaa ctg ggg gag ttg ggt gat gtc 403Thr Ala Ala Leu Asp Glu Ala Leu Lys Leu Gly Glu Leu Gly Asp Val
90 95 100gca agg att act gag gct ttg gct gtt ggt tct gtg cgt gga gcc cga 451Ala Arg Ile Thr Glu Ala Leu Ala Val Gly Ser Val Arg Gly Ala Arg
105 110 115gga aat tct gga gta gtc ctt agt cag gtc ctt cgc gct att gct cag 499Gly Asn Ser Gly Val Val Leu Ser Gln Val Leu Arg Ala Ile Ala Gln
120 125 130gca gct gct gac ggg gtt att gat ggc cac aca atc caa gaa gcg cta 547Ala Ala Ala Asp Gly Val Ile Asp Gly His Thr Ile Gln Glu Ala Leu
135 140 145tcc att gct cgc tcc cta gtt gat cgc gca att aca gat cct gtg gag 595Ser Ile Ala Arg Ser Leu Val Asp Arg Ala Ile Thr Asp Pro Val Glu150 155 160 165ggc act gtt gtc act gtg ttg cgt tct 622Gly Thr Val Val Thr Val Leu Arg Ser
170<210>518<211>174<212>PRT<213>谷氨酸棒杆菌<400>518Met Ser Ser Ser Glu Ser Ser Arg Ser Glu Gly Ser Gln Pro Ala Pro1 5 10 15Ser Val Gln Pro Glu Arg Arg Ala Asp Ser Thr Gly Ala Pro Ala Ala
20 25 30Ala Ser Lys Glu Ala Ser Gln Gln Met Asp Ala Ala Gly Val Leu Glu
35 40 45Trp Ala Arg Thr Ala Val Glu Gln Leu Ser Glu Arg Arg Ala Glu Ile
50 55 60Asn Ala Leu Asn Val Phe Pro Val Pro Asp Ala Asp Thr Gly Ser Asn65 70 75 80Met Thr Tyr Thr Met Thr Ala Ala Leu Asp Glu Ala Leu Lys Leu Gly
85 90 95Glu Leu Gly Asp Val Ala Arg Ile Thr Glu Ala Leu Ala Val Gly Ser
100 105 110Val Arg Gly Ala Arg Gly Asn Ser Gly Val Val Leu Ser Gln Val Leu
115 120 125Arg Ala Ile Ala Gln Ala Ala Ala Asp Gly Val Ile Asp Gly His Thr
130 135 140Ile Gln Glu Ala Leu Ser Ile Ala Arg Ser Leu Val Asp Arg Ala Ile145 150 155 160Thr Asp Pro Val Glu Gly Thr Val Val Thr Val Leu Arg Ser
165 170<210>519<211>1047<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1024)<223>RXC01212<400>519tttagaagcc acatgacata tgtcatgaaa attatgtgca aagtgcagta atactcctga 60catatggctc taccagcgcc aatgcgaagt aggaagaatt atg cct atg aca acg 115
Met Pro Met Thr Thr
1 5aca cca gca atc gac gta aca gac ctc gtg aga acc tac ggc gac tac 163Thr Pro Ala Ile Asp Val Thr Asp Leu Val Arg Thr Tyr Gly Asp Tyr
10 15 20acc gca gtc aag ggc ctg aat ttc cat gta cag cgc ggt gaa gta ttt 211Thr Ala Val Lys Gly Leu Asn Phe His Val Gln Arg Gly Glu Val Phe
25 30 35ggt ctg ctc ggc acc aac ggg gcc ggc aaa acc tcc acc ttg gaa gtc 259Gly Leu Leu Gly Thr Asn Gly Ala Gly Lys Thr Ser Thr Leu Glu Val
40 45 50atc gaa gga ctt tcc gca ccc agc tcc ggc acc gtg cgc atc tcc ggg 307Ile Glu Gly Leu Ser Ala Pro Ser Ser Gly Thr Val Arg Ile Ser Gly
55 60 65ctt gac ccc gtt gcc gac cgc gcg atc ctg cgc ccc gag ctc ggc atc 355Leu Asp Pro Val Ala Asp Arg Ala Ile Leu Arg Pro Glu Leu Gly Ile70 75 80 85atg ctg caa tca ggc ggc ctg cca tca cag ctc acc gtc gcc gaa acc 403Met Leu Gln Ser Gly Gly Leu Pro Ser Gln Leu Thr Val Ala Glu Thr
90 95 100atg gac atg tgg cac ggc acc tgc acg tat ccg cgc gcc att aaa gat 451Met Asp Met Trp His Gly Thr Cys Thr Tyr Pro Arg Ala Ile Lys Asp
105 110 115gtg ctt gcc gac gtc gac ctc cta cac cgc gaa aac gtc aag gtc ggc 499Val Leu Ala Asp Val Asp Leu Leu His Arg Glu Asn Val Lys Val Gly
120 125 130gcg ctt tcc gga ggc gaa caa cga cgc ctt gat ttg gcc tgc gca ctg 547Ala Leu Ser Gly Gly Glu Gln Arg Arg Leu Asp Leu Ala Cys Ala Leu
135 140 145ctt ggc gac ccc tca att ttg ttc ctc gac gaa ccc acc acc ggc ctc 595Leu Gly Asp Pro Ser Ile Leu Phe Leu Asp Glu Pro Thr Thr Gly Leu150 155 160 165gac cca gaa tct agg cgc cac acc tgg caa cgc ctg ctg gac ctg aaa 643Asp Pro Glu Ser Arg Arg His Thr Trp Gln Leu Leu Leu Asp Leu Lys
170 175 180cag cgc ggc gtc acc atg atg ctg acc acc cac tac ctg gag gaa gcc 691Gln Arg Gly Val Thr Met Met Leu Thr Thr His Tyr Leu Glu Glu Ala
185 190 195gaa ttc ctc tgc gac cgg att gcc atc atg aac gcc ggt gag atc gca 739Glu Phe Leu Cys Asp Arg Ile Ala Ile Met Asn Ala Gly Glu Ile Ala
200 205 210gtg gaa ggc acc ttg gat gaa ctg gtg gcc cgc gag aag tcg atc atc 787Val Glu Gly Thr Leu Asp Glu Leu Val Ala Arg Glu Lys Ser Ile Ile
215 220 225agt ttc gtg ctg cgt ggc ggg cag gtg gag ttg ccg gtc ttg agt ggg 835Ser Phe Val Leu Arg Gly Gly Gln Val Glu Leu Pro Val Leu Ser Gly230 235 240 245gct gaa atc atc cgc gac aac aac cac gtc cgc atc gcc acc acc acc 883Ala Glu Ile Ile Arg Asp Asn Asn His Val Arg Ile Ala Thr Thr Thr
250 255 260ctg cag cag cac acc tta gaa ata cgt acc tgg gct gca gag acc ggg 931Leu Gln Gln His Thr Leu Glu Ile Leu Thr Trp Ala Ala Glu Thr Gly
265 270 275atc gcg ctg gaa ggc ttc gct gca aaa ccc gcc acc ttg gaa tcc gta 979Ile Ala Leu Glu Gly Phe Ala Ala Lys Pro Ala Thr Leu Glu Ser Val
280 285 290ttc atg gac atc gcc tca ctc gag aac acc tcg ctg caa acc gcc 1024Phe Met Asp Ile Ala Ser Leu Glu Asn Thr Ser Leu Gln Thr Ala
295 300 305tagaatcttt aaggagacca caa 1047<210>520<211>308<212>PRT<213>谷氨酸棒杆菌<400>520Met Pro Met Thr Thr Thr Pro Ala Ile Asp Val Thr Asp Leu Val Arg1 5 10 15Thr Tyr Gly Asp Tyr Thr Ala Val Lys Gly Leu Asn Phe His Val Gln
20 25 30Arg Gly Glu Val Phe Gly Leu Leu Gly Thr Asn Gly Ala Gly Lys Thr
35 40 45Ser Thr Leu Glu Val Ile Glu Gly Leu Ser Ala Pro Ser Ser Gly Thr
50 55 60Val Arg Ile Ser Gly Leu Asp Pro Val Ala Asp Arg Ala Ile Leu Arg65 70 75 80Pro Glu Leu Gly Ile Met Leu Gln Ser Gly Gly Leu Pro Ser Gln Leu
85 90 95Thr Val Ala Glu Thr Met Asp Met Trp His Gly Thr Cys Thr Tyr Pro
100 105 110Arg Ala Ile Lys Asp Val Leu Ala Asp Val Asp Leu Leu His Arg Glu
115 120 125Asn Val Lys Val Gly Ala Leu Ser Gly Gly Glu Gln Arg Arg Leu Asp
130 135 140Leu Ala Cys Ala Leu Leu Gly Asp Pro Ser Ile Leu Phe Leu Asp Glu145 150 155 160Pro Thr Thr Gly Leu Asp Pro Glu Ser Arg Arg His Thr Trp Gln Leu
165 170 175Leu Leu Asp Leu Lys Gln Arg Gly Val Thr Met Met Leu Thr Thr His
180 185 190Tyr Leu Glu Glu Ala Glu Phe Leu Cys Asp Arg Ile Ala Ile Met Asn
195 200 205Ala Gly Glu Ile Ala Val Glu Gly Thr Leu Asp Glu Leu Val Ala Arg
210 215 220Glu Lys Ser Ile Ile Ser Phe Val Leu Arg Gly Gly Gln Val Glu Leu225 230 235 240Pro Val Leu Ser Gly Ala Glu Ile Ile Arg Asp Asn Asn His Val Arg
245 250 255Ile Ala Thr Thr Thr Leu Gln Gln His Thr Leu Glu Ile Leu Thr Trp
260 265 270Ala Ala Glu Thr Gly Ile Ala Leu Glu Gly Phe Ala Ala Lys Pro Ala
275 280 285Thr Leu Glu Ser Val Phe Met Asp Ile Ala Ser Leu Glu Asn Thr Ser
290 295 300Leu Gln Thr Ala305<210>521<211>1131<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1108)<223>RXC01306<400>521tgttttagag gtagagcgaa acgtgcctgt aaaactggcg ctcgctgtgc tcgaagacca 60cccagacaat caccccgctc cgaaggagaa tcgctaagcc atg act gaa tgg tat 115
Met Thr Glu Trp Tyr
1 5gtc gtt tta ccc gcc act att cta ctc atc gcg ctg tct gcg ttt ttc 163Val Val Leu Pro Ala Thr Ile Leu Leu Ile Ala Leu Ser Ala Phe Phe
10 15 20gtc atc att gag ttc gct ttg ctt gca gct agg cgg aac cgg tta gag 211Val Ile Ile Glu Phe Ala Leu Leu Ala Ala Arg Arg Asn Arg Leu Glu
25 30 35gag act gtg gaa acc tcg cgg tct tcc cgc gct gcg ttg cga agc ctc 259Glu Thr Val Glu Thr Ser Arg Ser Ser Arg Ala Ala Leu Arg Ser Leu
40 45 50aat gaa ctt act ctc atg ctc gcg ggc gcg cag ttg gga atc acc atg 307Asn Glu Leu Thr Leu Met Leu Ala Gly Ala Gln Leu Gly Ile Thr Met
55 60 65gtg act ttc gcg ttg ggt gct atc acg aag ccg tgg gtt cat tat gct 355Val Thr Phe Ala Leu Gly Ala Ile Thr Lys Pro Trp Val His Tyr Ala70 75 80 85ttg atg ccg ctc ttc gaa tgg gcg cgt ata ccg ctg gtt atg gca gat 403Leu Met Pro Leu Phe Glu Trp Ala Arg Ile Pro Leu Val Met Ala Asp
90 95 100gtc att gcg ttt att ttg tcg ctg ttt atc gta acg ttt ctg cac ttg 451Val Ile Ala Phe Ile Leu Ser Leu Phe Ile Val Thr Phe Leu His Leu
105 110 115gtc atc ggc gaa atg gct ccg aaa tcc tgg gca atc gcg cat ccg gag 499Val Ile Gly Glu Met Ala Pro Lys Ser Trp Ala Ile Ala His Pro Glu
120 125 130acg gca ctt cga act atc gcg att ccc gca cgg ggc ttc att aac ctg 547Thr Ala Leu Arg Thr Ile Ala Ile Pro Ala Arg Gly Phe Ile Asn Leu
135 140 145ttt cgt cca ttg ctg cag tgg atc aac aaa atg gcg aac gat ttg gtc 595Phe Arg Pro Leu Leu Gln Trp Ile Asn Lys Met Ala Asn Asp Leu Val150 155 160 165cgc aaa gtt ggt gaa act ccc gtt gat cga gct gca gct ggt ggc tat 643Arg Lys Val Gly Glu Thr Pro Val Asp Arg Ala Ala Ala Gly Gly Tyr
170 175 180gac acc gat acc ctc cat gcc ctc att gag cat tcc cga gaa act ggc 691Asp Thr Asp Thr Leu His Ala Leu Ile Glu His Ser Arg Glu Thr Gly
185 190 195gct ctg gat cag caa tcc gcc gcc caa atc agc gga att atc aag ctg 739Ala Leu Asp Gln Gln Ser Ala Ala Gln Ile Ser Gly Ile Ile Lys Leu
200 205 210gat aaa atc acg gtc ggt caa acc ctg acc gca tct cca ttt acg cac 787Asp Lys Ile Thr Val Gly Gln Thr Leu Thr Ala Ser Pro Phe Thr His
215 220 225agc gcc agc gcc acg gtt gct gag gtg caa gcc gca gct cag cgc agt 835Ser Ala Ser Ala Thr Val Ala Glu Val Gln Ala Ala Ala Gln Arg Ser230 235 240 245ggc agc ttg cgt gtg ctt atc gac gcc ccc tcc cac ctt ttc cca cac 883Gly Ser Leu Arg Val Leu Ile Asp Ala Pro Ser His Leu Phe Pro His
250 255 260gtc att cat gtg cga gac acc ctt ggt gcc tcg cca gac gag aag gct 931Val Ile His Val Arg Asp Thr Leu Gly Ala Ser Pro Asp Glu Lys Ala
265 270 275tcg aag tgg tct cgc cca atc ctc acc gtt gct gag acc gac acg tta 979Ser Lys Trp Ser Arg Pro Ile Leu Thr Val Ala Glu Thr Asp Thr Leu
280 285 290cac caa gcg ctg gaa tac atg cgg gag cat aac gag cag atc agt gcg 1027His Gln Ala Leu Glu Tyr Met Arg Glu His Asn Glu Gln Ile Ser Ala
295 300 305gtg ctt tcc gct gat ggg aaa acg gtg ctt ggt gta ata act tgg gat 1075Val Leu Ser Ala Asp Gly Lys Thr Val Leu Gly Val Ile Thr Trp Asp310 315 320 325cac atc ttg aaa tac ctg tgg cct gca tcg gtg tagctaattt gaggtgcgct 1128His Ile Leu Lys Tyr Leu Trp Pro Ala Ser Val
330 335gaa 1131<210>522<211>336<212>PRT<213>谷氨酸棒杆菌<400>522Met Thr Glu Trp Tyr Val Val Leu Pro Ala Thr Ile Leu Leu Ile Ala1 5 10 15Leu Ser Ala Phe Phe Val Ile Ile Glu Phe Ala Leu Leu Ala Ala Arg
20 25 30Arg Asn Arg Leu Glu Glu Thr Val Glu Thr Ser Arg Ser Ser Arg Ala
35 40 45Ala Leu Arg Ser Leu Asn Glu Leu Thr Leu Met Leu Ala Gly Ala Gln
50 55 60Leu Gly Ile Thr Met Val Thr Phe Ala Leu Gly Ala Ile Thr Lys Pro65 70 75 80Trp Val His Tyr Ala Leu Met Pro Leu Phe Glu Trp Ala Arg Ile Pro
85 90 95Leu Val Met Ala Asp Val Ile Ala Phe Ile Leu Ser Leu Phe Ile Val
100 105 110Thr Phe Leu His Leu Val Ile Gly Glu Met Ala Pro Lys Ser Trp Ala
115 120 125Ile Ala His Pro Glu Thr Ala Leu Arg Thr Ile Ala Ile Pro Ala Arg
130 135 140Gly Phe Ile Asn Leu Phe Arg Pro Leu Leu Gln Trp Ile Asn Lys Met145 150 155 160Ala Asn Asp Leu Val Arg Lys Val Gly Glu Thr Pro Val Asp Arg Ala
165 170 175Ala Ala Gly Gly Tyr Asp Thr Asp Thr Leu His Ala Leu Ile Glu His
180 185 190Ser Arg Glu Thr Gly Ala Leu Asp Gln Gln Ser Ala Ala Gln Ile Ser
195 200 205Gly Ile Ile Lys Leu Asp Lys Ile Thr Val Gly Gln Thr Leu Thr Ala
210 215 220Ser Pro Phe Thr His Ser Ala Ser Ala Thr Val Ala Glu Val Gln Ala225 230 235 240Ala Ala Gln Arg Ser Gly Ser Leu Arg Val Leu Ile Asp Ala Pro Ser
245 250 255His Leu Phe Pro His Val Ile His Val Arg Asp Thr Leu Gly Ala Ser
260 265 270Pro Asp Glu Lys Ala Ser Lys Trp Ser Arg Pro Ile Leu Thr Val Ala
275 280 285Glu Thr Asp Thr Leu His Gln Ala Leu Glu Tyr Met Arg Glu His Asn
290 295 300Glu Gln Ile Ser Ala Val Leu Ser Ala Asp Gly Lys Thr Val Leu Gly305 310 315 320Val Ile Thr Trp Asp His Ile Leu Lys Tyr Leu Trp Pro Ala Ser Val
325 330 355<210>523<211>513<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(490)<223>RXC01366<400>523atgcatgaaa acaaattcta tgtgtgttga gctgccaaaa ggggttggcg cgccgatgat 60gactgtccaa acctaaacca aaggtctaaa ctttggcttc gtg agt cag ttt cgt 115
Val Ser Gln Phe Arg
1 5cgt tgt tcc cgc cct ggt tgt ggc aag cct gcc gtc gca acc ctc acc 163Arg Cys Ser Arg Pro Gly Cys Gly Lys Pro Ala Val Ala Thr Leu Thr
10 15 20tac gca tat tcg gat tcc act gcg gtg gtt ggt cct ttg gcg cct gca 211Tyr Ala Tyr Ser Asp Ser Thr Ala Val Val Gly Pro Leu Ala Pro Ala
25 30 35gca gag ccc cat agt tgg gat ctg tgt gag cat cat gcc gag cgt att 259Ala Glu Pro His Ser Trp Asp Leu Cys Glu His His Ala Glu Arg Ile
40 45 50act gcg ccc ctt ggt tgg gag atg ctg cgg gtg aac gac atc aaa gtc 307Thr Ala Pro Leu Gly Trp Glu Met Leu Arg Val Asn Asp Ile Lys Val
55 60 65gat gac gat gag gat ctg acg gct ctt gct cag gct gtt cgt gag gct 355Asp Asp Asp Glu Asp Leu Thr Ala Leu Ala Gln Ala Val Arg Glu Ala70 75 80 85gga cgc act gtg agt ggt ctg gtt cct gaa gac gaa gtg ggc ggc aac 403Gly Arg Thr Val Ser Gly Leu Val Pro Glu Asp Glu Val Gly Gly Asn
90 95 100cat ccg gtg aac cgg agt gcg cgg atc gcg gaa cag aag gtt cac cgc 451His Pro Val Asn Arg Ser Ala Arg Ile Ala Glu Gln Lys Val His Arg
105 110 115agg ggt cat ctc tat gtt gtg cct gat cag gac gaa tca taaggtttgc 500Arg Gly His Leu Tyr Val Val Pro Asp Gln Asp Glu Ser
120 125 130tattcggatt gga 513<210>524<211>130<212>PRT<213>谷氨酸棒杆菌<400>524Val Ser Gln Phe Arg Arg Cys Ser Arg Pro Gly Cys Gly Lys Pro Ala1 5 10 15Val Ala Thr Leu Thr Tyr Ala Tyr Ser Asp Ser Thr Ala Val Val Gly
20 25 30Pro Leu Ala Pro Ala Ala Glu Pro His Ser Trp Asp Leu Cys Glu His
35 40 45His Ala Glu Arg Ile Thr Ala Pro Leu Gly Trp Glu Met Leu Arg Val
50 55 60Asn Asp Ile Lys Val Asp Asp Asp Glu Asp Leu Thr Ala Leu Ala Gln65 70 75 80Ala Val Arg Glu Ala Gly Arg Thr Val Ser Gly Leu Val Pro Glu Asp
85 90 95Glu Val Gly Gly Asn His Pro Val Asn Arg Ser Ala Arg Ile Ala Glu
100 105 110Gln Lys Val His Arg Arg Gly His Leu Tyr Val Val Pro Asp Gln Asp
115 120 125Glu Ser
130<210>525<211>614<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(591)<223>RXC01372<400>525cag gac acc ttc gtc ctt ccc acc ttg ccc acg gcc gca ggc ttg tcg 48Gln Asp Thr Phe Val Leu Pro Thr Leu Pro Thr Ala Ala Gly Leu Ser1 5 10 15cct gcc cgc atc gtg gcg tcg ata agc act ctt tta gat ctt tta gaa 96Pro Ala Arg Ile Val Ala Ser Ile Ser Thr Leu Leu Asp Leu Leu Glu
20 25 30gca gac ccc agc att att tcc gac cgc ttg gaa cac ctc gcc gac tgc 144Ala Asp Pro Ser Ile Ile Ser Asp Arg Leu Glu His Leu Ala Asp Cys
35 40 45att gat gag gaa gtg gaa tcg cta tcg ccg gaa cgt gac gaa cta gtc 192Ile Asp Glu Glu Val Glu Ser Leu Ser Pro Glu Arg Asp Glu Leu Val
50 55 60aat ccc ggc cga aaa ctg cgc gca tac gta gat cac gca cgg atc gtg 240Asn Pro Gly Arg Lys Leu Arg Ala Tyr Val Asp His Ala Arg Ile Val65 70 75 80cat acc ggc cga act gat gtg gga ctc gcg att gcc aac gtt atc gcc 288His Thr Gly Arg Thr Asp Val Gly Leu Ala Ile Ala Asn Val Ile Ala
85 90 95cca atc tgg acc cga cga ggc ctg gta tca gcc gtg ctg gat ttt ccc 336Pro Ile Trp Thr Arg Arg Gly Leu Val Ser Ala Val Leu Asp Phe Pro
100 105 110gag ctc atg gaa tca ttg ccg gaa ctc cgc gga ccc gag cca att acc 384Glu Leu Met Glu Ser Leu Pro Glu Leu Arg Gly Pro Glu Pro Ile Thr
115 120 125gac gay ata ttc cat gac cca ttc ata gat gac gaa ccc ggg gtg gta 432Asp Asp Ile Phe His Asp Pro Phe Ile Asp Asp Glu Pro Gly Val Val
130 135 140ccg ttt agg gct gtt gtc tgg gcc gaa gag gaa ccc gga atc ccc gat 480Pro Phe Arg Ala Val Val Trp Ala Glu Glu Glu Pro Gly Ile Pro Asp145 150 155 160gcc atg gcg caa agc tgc gac gga cct agc aaa ggg gcg ctg aca caa 528Ala Met Ala Gln Ser Cys Asp Gly Pro Ser Lys Gly Ala Leu Thr Gln
165 170 175gca ctg cgt ttg ctg gtg cgc gga cag tca gcc acg acc tat tcc att 576Ala Leu Arg Leu Leu Val Arg Gly Gln Ser Ala Thr Thr Tyr Ser Ile
180 185 190gaa gaa aag gac ttg taaatggagc tattggaagg ctc 614Glu Glu Lys Asp Leu
195<210>526<211>197<212>PRT<213>谷氨酸棒杆菌<400>526Gln Asp Thr Phe Val Leu Pro Thr Leu Pro Thr Ala Ala Gly Leu Ser1 5 10 15Pro Ala Arg Ile Val Ala Ser Ile Ser Thr Leu Leu Asp Leu Leu Glu
20 25 30Ala Asp Pro Ser Ile Ile Ser Asp Arg Leu Glu His Leu Ala Asp Cys
35 40 45Ile Asp Glu Glu Val Glu Ser Leu Ser Pro Glu Arg Asp Glu Leu Val
50 55 60Asn Pro Gly Arg Lys Leu Arg Ala Tyr Val Asp His Ala Arg Ile Val65 70 75 80His Thr Gly Arg Thr Asp Val Gly Leu Ala Ile Ala Asn Val Ile Ala
85 90 95Pro Ile Trp Thr Arg Arg Gly Leu Val Ser Ala Val Leu Asp Phe Pro
100 105 110Glu Leu Met Glu Ser Leu Pro Glu Leu Arg Gly Pro Glu Pro Ile Thr
115 120 125Asp Asp Ile Phe His Asp Pro Phe Ile Asp Asp Glu Pro Gly Val Val
130 135 140Pro Phe Arg Ala Val Val Trp Ala Glu Glu Glu Pro Gly Ile Pro Asp145 150 155 160Ala Met Ala Gln Ser Cys Asp Gly Pro Ser Lys Gly Ala Leu Thr Gln
165 170 175Ala Leu Arg Leu Leu Val Arg Gly Gln Ser Ala Thr Thr Tyr Ser Ile
180 185 190Glu Glu Lys Asp Leu
195<210>527<211>669<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(646)<223>RXC01659<400>527ttgatgcgcg ccgcggtgag cagcctgccg atccagagcg tgaagaatct gatgatttag 60tcgagattga taccgtctct ggattccgcc tgctcagtac gtg gcg ggg gtt gcg 115
Val Ala Gly Val Ala
1 5cag cgt ttt gtc gac gag cgc att cac gtc ggt ttg gat tcc atg cca 163Gln Arg Phe Val Asp Glu Arg Ile His Val Gly Leu Asp Ser Met Pro
10 15 20gaa gct gtg act gct gtg tgg atg gaa tct gat tgg gtg ttg gcg gaa 211Glu Ala Val Thr Ala Val Trp Met Glu Ser Asp Trp Val Leu Ala Glu
25 30 35acc atc aag ggt tcc acg cct tcc gat tgg gaa gag att ttg cgg ccg 259Thr Ile Lys Gly Ser Thr Pro Ser Asp Trp Glu Glu Ile Leu Arg Pro
40 45 50ttg gcg ctg crc acg gac gcg tct ttc acg ttg cca cct cgt tcc acg 307Leu Ala Leu Leu Thr Asp Ala Ser Phe Thr Leu Pro Pro Arg Ser Thr
55 60 65cgt gcg caa acc ttg gat ttg aag cat ttg gaa cca agc cgt ctg aag 355Arg Ala Gln Thr Leu Asp Leu Lys His Leu Glu Pro Ser Arg Leu Lys70 75 80 85ccg gag cag cca gaa aag cca gcg ttt act ccc aat gct tcg gaa gaa 403Pro Glu Gln Pro Glu Lys Pro Ala Phe Thr Pro Asn Ala Ser Glu Glu
90 95 100gat ttg tct cag ccg ttg gtg atc cgc ccc gag gag ccg ttg cag atg 451Asp Leu Ser Gln Pro Leu Val Ile Arg Pro Glu Glu Pro Leu Gln Met
105 110 115ccg gtt cgc ggt gtg cag gaa agc cgc gga gtg gtc gag cca cgg tca 499Pro Val Arg Gly Val Gln Glu Ser Arg Gly Val Val Glu Pro Arg Ser
120 125 130ttg ggt gcg gat gat gtg gag tcg att gcg gag ggc gat cca gag cgt 547Leu Gly Ala Asp Asp Val Glu Ser Ile Ala Glu Gly Asp Pro Glu Arg
135 140 145ccg agc gat ctt tat ggc acg cgt gtg ctg cgt gat ctc aat ggt cag 595Pro Ser Asp Leu Tyr Gly Thr Arg Val Leu Arg Asp Leu Asn Gly Gln150 155 160 165tcc agt att ttc caa gat tcc acc gac gcg gat gag cca cca aaa aag 643Ser Ser Ile Phe Gln Asp Ser Thr Asp Ala Asp Glu Pro Pro Lys Lys
170 175 180tgg tagaaaactg gtgtttttcg gcc 669Trp<210>528<211>182<212>PRT<213>谷氨酸棒杆菌<400>528Val Ala Gly Val Ala Gln Arg Phe Val Asp Glu Arg Ile His Val Gly1 5 10 15Leu Asp Ser Met Pro Glu Ala Val Thr Ala Val Trp Met Glu Ser Asp
20 25 30Trp Val Leu Ala Glu Thr Ile Lys Gly Ser Thr Pro Ser Asp Trp Glu
35 40 45Glu Ile Leu Arg Pro Leu Ala Leu Leu Thr Asp Ala Ser Phe Thr Leu
50 55 60Pro Pro Arg Ser Thr Arg Ala Gln Thr Leu Asp Leu Lys His Leu Glu65 70 75 80Pro Ser Arg Leu Lys Pro Glu Gln Pro Glu Lys Pro Ala Phe Thr Pro
85 90 95Asn Ala Ser Glu Glu Asp Leu Ser Gln Pro Leu Val Ile Arg Pro Glu
100 105 110Glu Pro Leu Gln Met Pro Val Arg Gly Val Gln Glu Ser Arg Gly Val
115 120 125Val Glu Pro Arg Ser Leu Gly Ala Asp Asp Val Glu Ser Ile Ala Glu
130 135 140Gly Asp Pro Glu Arg Pro Ser Asp Leu Tyr Gly Thr Arg Val Leu Arg145 150 155 160Asp Leu Asn Gly Gln Ser Ser Ile Phe Gln Asp Ser Thr Asp Ala Asp
165 170 175Glu Pro Pro Lys Lys Trp
180<210>529<211>981<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(958)<223>RXC01663<400>529tattttgctg gttggtcaca gtggagcgct aaccccgaga atcctatcga ggcctaaaat 60cgtggcttga gtacgcactg ccagtaaggt gtgtgatgtg atg gaa ata agt gtc 115
Met Glu Ile Ser Val
1 5ttg atc atc gcc gca ctg atc ttg gtg gca ggc atc gta ctg tgg cgc 163Leu Ile Ile Ala Ala Leu Ile Leu Val Ala Gly Ile Val Leu Trp Arg
10 15 20gcg gac tcg tct aaa cag gca gct aaa aag gct gaa tca cct gtg ggc 211Ala Asp Ser Ser Lys Gln Ala Ala Lys Lys Ala Glu Ser Pro Val Gly
25 30 35tca gtc gca cct gcg ccc gtg ctg gtt gaa gaa gag ccg gac cct gag 259Ser Val Ala Pro Ala Pro Val Leu Val Glu Glu Glu Pro Asp Pro Glu
40 45 50ttt gag cca gaa ctg gac cct gaa cca gaa gcg caa cca gaa cca gag 307Phe Glu Pro Glu Leu Asp Pro Glu Pro Glu Ala Gln Pro Glu Pro Glu
55 60 65ctg gaa gtt gcg cct aga ttt gcg cca gaa cca gtt caa gat ctt gag 355Leu Glu Val Ala Pro Arg Phe Ala Pro Glu Pro Val Gln Asp Leu Glu70 75 80 85ccg gat cag gct gag gac att tat ttt gat gat tcc cct gaa ctc gat 403Pro Asp Gln Ala Glu Asp Ile Tyr Phe Asp Asp Ser Pro Glu Leu Asp
90 95 100gct gat gtt gaa aat gcc ttg gct gag ctt act gag gta gaa gac tac 451Ala Asp Val Glu Asn Ala Leu Ala Glu Leu Thr Glu Val Glu Asp Tyr
105 110 115ccg gaa gag cca gtg cag tct gag caa cct caa gcc cct gcc acg gcg 499Pro Glu Glu Pro Val Gln Ser Glu Gln Pro Gln Ala Pro Ala Thr Ala
120 125 130gag gta gct gcg gac gag gag caa cgg ggc gtc gat aag cat tcg ttt 547Glu Val Ala Ala Asp Glu Glu Gln Arg Gly Val Asp Lys His Ser Phe
135 140 145ttg agc tct ttg cct ggt tcg cag cgc cgg gag cgc cga aac tgg gcg 595Leu Ser Ser Leu Pro Gly Ser Gln Arg Arg Glu Arg Arg Asn Trp Ala150 155 160 165gcg aag cac cac ttc gat ttc atc aag gaa gat gcc ttt ttg acc gat 643Ala Lys His His Phe Asp Phe Ile Lys Glu Asp Ala Phe Leu Thr Asp
170 175 180gaa tgg tca agg ggt gcg gca tcg act ggt gcc gtt gca cgt gat gtg 691Glu Trp Ser Arg Gly Ala Ala Ser Thr Gly Ala Val Ala Arg Asp Val
185 190 195gtc agt ggc atg gct gaa gga tat gaa acg cat ctg gtg gat ttg gcg 739Val Ser Gly Met Ala Glu Gly Tyr Glu Thr His Leu Val Asp Leu Ala
200 205 210ggc gtg ccc gtg atg gcg atg cgc cgt gga att acc tct gac gtg gtc 787Gly Val Pro Val Met Ala Met Arg Arg Gly Ile Thr Ser Asp Val Val
215 220 225att gat gcg cgc cgc ggt gag cag cct gcc gat cca gag cgt gaa gaa 835Ile Asp Ala Arg Arg Gly Glu Gln Pro Ala Asp Pro Glu Arg Glu Glu230 235 240 245tct gat gat tta gtc gag att gat acc gtc tct gga ttc cgc ctg ctc 883Ser Asp Asp Leu Val Glu Ile Asp Thr Val Ser Gly Phe Arg Leu Leu
250 255 260agt acg tgg cgg ggg ttg cgc agc gtt ttg tcg acg agc gca ttc acg 931Ser Thr Trp Arg Gly Leu Arg Ser Val Leu Ser Thr Ser Ala Phe Thr
265 270 275tcg gtt tgg att cca tgc cag aag ctg tgactgctgt gtggatggaa 978Ser Val Trp Ile Pro Cys Gln Lys Leu
280 285tct 981<210>530<211>286<212>PRT<213>谷氨酸棒杆菌<400>530Met Glu Ile Ser Val Leu Ile Ile Ala Ala Leu Ile Leu Val Ala Gly1 5 10 15Ile Val Leu Trp Arg Ala Asp Ser Ser Lys Gln Ala Ala Lys Lys Ala
20 25 30Glu Ser Pro Val Gly Ser Val Ala Pro Ala Pro Val Leu Val Glu Glu
35 40 45Glu Pro Asp Pro Glu Phe Glu Pro Glu Leu Asp Pro Glu Pro Glu Ala
50 55 60Gln Pro Glu Pro Glu Leu Glu Val Ala Pro Arg Phe Ala Pro Glu Pro65 70 75 80Val Gln Asp Leu Glu Pro Asp Gln Ala Glu Asp Ile Tyr Phe Asp Asp
85 90 95Ser Pro Glu Leu Asp Ala Asp Val Glu Asn Ala Leu Ala Glu Leu Thr
100 105 110Glu Val Glu Asp Tyr Pro Glu Glu Pro Val Gln Ser Glu Gln Pro Gln
115 120 125Ala Pro Ala Thr Ala Glu Val Ala Ala Asp Glu Glu Gln Arg Gly Val
130 135 140Asp Lys His Ser Phe Leu Ser Ser Leu Pro Gly Ser Gln Arg Arg Glu145 150 155 160Arg Arg Asn Trp Ala Ala Lys His His Phe Asp Phe Ile Lys Glu Asp
165 170 175Ala Phe Leu Thr Asp Glu Trp Ser Arg Gly Ala Ala Ser Thr Gly Ala
180 185 190Val Ala Arg Asp Val Val Ser Gly Met Ala Glu Gly Tyr Glu Thr His
195 200 205Leu Val Asp Leu Ala Gly Val Pro Val Met Ala Met Arg Arg Gly Ile
210 215 220Thr Ser Asp Val Val Ile Asp Ala Arg Arg Gly Glu Gln Pro Ala Asp225 230 235 240Pro Glu Arg Glu Glu Ser Asp Asp Leu Val Glu Ile Asp Thr Val Ser
245 250 255Gly Phe Arg Leu Leu Ser Thr Trp Arg Gly Leu Arg Ser Val Leu Ser
260 265 270Thr Ser Ala Phe Thr Ser Val Trp Ile Pro Cys Gln Lys Leu
275 280 285<210>531<211>1545<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1522)<223>RXC01693<400>531aggagactgg ctggattatt ggctggtttt cttgggaaat cgtcatgggc attaatccta 60gtcccaacaa ttgccagaac ccgaacacta ggcttgaacc atg aat act gca cct 115
Met Asn Thr Ala Pro
1 5ttc aaa ctc gaa gct gac ttc gca tca gcc ctg ccc acc atg gca gcc 163Phe Lys Leu Glu Ala Asp Phe Ala Ser Ala Leu Pro Thr Met Ala Ala
10 15 20ccc tgg caa ggt gag gaa gcc ccc aac cct gag ctc gtg att tta aat 211Pro Trp Gln Gly Glu Glu Ala Pro Asn Pro Glu Leu Val Ile Leu Asn
25 30 35gac gac ctc gcc tac agc ctc ggg ctt gat ccg aca tgg ctt cgc aca 259Asp Asp Leu Ala Tyr Ser Leu Gly Leu Asp Pro Thr Trp Leu Arg Thr
40 45 50cct gag ggc gtt caa ttt ctt ctc gga ctc aac ccc gag ccc tta aca 307Pro Glu Gly Val Gln Phe Leu Leu Gly Leu Asn Pro Glu Pro Leu Thr
55 60 65aaa gca gtt gcg cag gcc tat tcc ggc cac caa ttc gga cag ttt gtg 355Lys Ala Val Ala Gln Ala Tyr Ser Gly His Gln Phe Gly Gln Phe Val70 75 80 85gca agc ctt ggt gat ggc cga gcg ctt ctt ctc ggc gaa gcc cgc tca 403Ala Ser Leu Gly Asp Gly Arg Ala Leu Leu Leu Gly Glu Ala Arg Ser
90 95 100gct gac ggc gta ctg cat gat atc cac ctc aaa gga tct gga cga acc 451Ala Asp Gly Val Leu His Asp Ile His Leu Lys Gly Ser Gly Arg Thr
105 110 115caa ttc tcc cga gga gcc gat gga cgc gcc gtc ctt ggc ccc gtc tta 499Gln Phe Ser Arg Gly Ala Asp Gly Arg Ala Val Leu Gly Pro Val Leu
120 125 130cgc gaa tac atc atc tcc gaa gcg atg cat gca ctt ggt gtt ccc acc 547Arg Glu Tyr Ile Ile Ser Glu Ala Met His Ala Leu Gly Val Pro Thr
135 140 145acc agg tca ctt gca gta att agc acc ggt agg aaa atc caa cga gga 595Thr Arg Ser Leu Ala Val Ile Ser Thr Gly Arg Lys Ile Gln Arg Gly150 155 160 165agc gta gcc cca ggc gca gtc ctt gtt cga gta gca acc agc ctc att 643Ser Val Ala Pro Gly Ala Val Leu Val Arg Val Ala Thr Ser Leu Ile
170 175 180cga gtc gga tcc ttc caa tac tcc aac atc tct ggt ggc atc gaa cta 691Arg Val Gly Ser Phe Gln Tyr Ser Asn Ile Ser Gly Gly Ile Glu Leu
185 190 195tct caa cac ctg gcg aac tat acg atc acc agg cat ttc cct tcg ttg 739Ser Gln His Leu Ala Asn Tyr Thr Ile Thr Arg His Phe Pro Ser Leu
200 205 210gta gct gaa cta tcc gca cca acc ccc gca act tat gta tca ctg ttt 787Val Ala Glu Leu Ser Ala Pro Thr Pro Ala Thr Tyr Val Ser Leu Phe
215 220 225aaa gcg att ctt cag cgc caa gca gac acc gtt gga aaa tgg acc agg 835Lys Ala Ile Leu Gln Arg Gln Ala Asp Thr Val Gly Lys Trp Thr Arg230 235 240 245ctg ggt ttc gtt cac gga gcc ctc aac aca gac aac acg ttg ata tcc 883Leu Gly Phe Val His Gly Ala Leu Asn Thr Asp Asn Thr Leu Ile Ser
250 255 260gga gaa act gtt gac tac ggc cca tgc gct ttc atg gag cgc tac cgt 931Gly Glu Thr Val Asp Tyr Gly Pro Cys Ala Phe Met Glu Arg Tyr Arg
265 270 275ggc gac gcg aaa ttt agc tcc atc gac act tat ggt cgc tac aaa ttt 979Gly Asp Ala Lys Phe Ser Ser Ile Asp Thr Tyr Gly Arg Tyr Lys Phe
280 285 290gaa aac caa cct atg atc ctc gga tgg aac atg gcc cgc ctc gta gaa 1027Glu Asn Gln Pro Met Ile Leu Gly Trp Asn Met Ala Arg Leu Val Glu
295 300 305acc ctc ctc cca ctc ctg ggc gcc aca cca gac gaa ggc atg aca gca 1075Thr Leu Leu Pro Leu Leu Gly Ala Thr Pro Asp Glu Gly Met Thr Ala310 315 320 325gcc caa gaa gct ctc gta gaa ttc gat gac ctc tgc gaa caa gca atc 1123Ala Gln Glu Ala Leu Val Glu Phe Asp Asp Leu Cys Glu Gln Ala Ile
330 335 340cga aaa gaa ttc gcc act gca ctg ggc ctt gac gag tca gac acc ggc 1171Arg Lys Glu Phe Ala Thr Ala Leu Gly Leu Asp Glu Ser Asp Thr Gly
345 350 355acg gta gag cag ttc cgt gaa ctg ctc tac ctc cat aac ccc gac atc 1219Thr Val Glu Gln Phe Arg Glu Leu Leu Tyr Leu His Asn Pro Asp Ile
360 365 370acc acg ctg ctg cgc gca ctc acc gac aac acc gca cca ccg agt ggc 1267Thr Thr Leu Leu Arg Ala Leu Thr Asp Asn Thr Ala Pro Pro Ser Gly
375 380 385ttt gaa gca ttc gtt cac gac tgg aaa acc caa gac cca gat atc gaa 1315Phe Glu Ala Phe Val His Asp Trp Lys Thr Gln Asp Pro Asp Ile Glu390 395 400 405gca atg cga gca gta aat cca ctt ttc att cca cgc aat cac ctc gtg 1363Ala Met Arg Ala Val Asn Pro Leu Phe Ile Pro Arg Asn His Leu Val
410 415 420gaa gct gct ctc gca gac gca gtt gaa ggg aat cta gaa aag ttc cac 1411Glu Ala Ala Leu Ala Asp Ala Val Glu Gly Asn Leu Glu Lys Phe His
425 430 435gaa ctc ctc gct gct gtc acc aat cct ttt gat cca act gcg ggc ccc 1459Glu Leu Leu Ala Ala Val Thr Asn Pro Phe Asp Pro Thr Ala Gly Pro
440 445 450gat gaa cta cgc ctg cca agc gaa gaa gga ttt gaa gaa gac tac atg 1507Asp Glu Leu Arg Leu Pro Ser Glu Glu Gly Phe Glu Glu Asp Tyr Met
455 460 465acc ttc tgc ggt acc taggacagat ggtggggcag acg 1545Thr Phe Cys Gly Thr470<210>532<211>474<212>PRT<213>谷氨酸棒杆菌<400>532Met Asn Thr Ala Pro Phe Lys Leu Glu Ala Asp Phe Ala Ser Ala Leu1 5 10 15Pro Thr Met Ala Ala Pro Trp Gln Gly Glu Glu Ala Pro Asn Pro Glu
20 25 30Leu Val Ile Leu Asn Asp Asp Leu Ala Tyr Ser Leu Gly Leu Asp Pro
35 40 45Thr Trp Leu Arg Thr Pro Glu Gly Val Gln Phe Leu Leu Gly Leu Asn
50 55 60Pro Glu Pro Leu Thr Lys Ala Val Ala Gln Ala Tyr Ser Gly His Gln65 70 75 80Phe Gly Gln Phe Val Ala Ser Leu Gly Asp Gly Arg Ala Leu Leu Leu
85 90 95Gly Glu Ala Arg Ser Ala Asp Gly Val Leu His Asp Ile His Leu Lys
100 105 110Gly Ser Gly Arg Thr Gln Phe Ser Arg Gly Ala Asp Gly Arg Ala Val
115 120 125Leu Gly Pro Val Leu Arg Glu Tyr Ile Ile Ser Glu Ala Met His Ala
130 135 140Leu Gly Val Pro Thr Thr Arg Ser Leu Ala Val Ile Ser Thr Gly Arg145 150 155 160Lys Ile Gln Arg Gly Ser Val Ala Pro Gly Ala Val Leu Val Arg Val
165 170 175Ala Thr Ser Leu Ile Arg Val Gly Ser Phe Gln Tyr Ser Asn Ile Ser
180 185 190Gly Gly Ile Glu Leu Ser Gln His Leu Ala Asn Tyr Thr Ile Thr Arg
195 200 205His Phe Pro Ser Leu Val Ala Glu Leu Ser Ala Pro Thr Pro Ala Thr
210 215 220Tyr Val Ser Leu Phe Lys Ala Ile Leu Gln Arg Gln Ala Asp Thr Val225 230 235 240Gly Lys Trp Thr Arg Leu Gly Phe Val His Gly Ala Leu Asn Thr Asp
245 250 255Asn Thr Leu Ile Ser Gly Glu Thr Val Asp Tyr Gly Pro Cys Ala Phe
260 265 270Met Glu Arg Tyr Arg Gly Asp Ala Lys Phe Ser Ser Ile Asp Thr Tyr
275 280 285Gly Arg Tyr Lys Phe Glu Asn Gln Pro Met Ile Leu Gly Trp Asn Met
290 295 300Ala Arg Leu Val Glu Thr Leu Leu Pro Leu Leu Gly Ala Thr Pro Asp305 310 315 320Glu Gly Met Thr Ala Ala Gln Glu Ala Leu Val Glu Phe Asp Asp Leu
325 330 335Cys Glu Gln Ala Ile Arg Lys Glu Phe Ala Thr Ala Leu Gly Leu Asp
340 345 350Glu Ser Asp Thr Gly Thr Val Glu Gln Phe Arg Glu Leu Leu Tyr Leu
355 360 365His Asn Pro Asp Ile Thr Thr Leu Leu Arg Ala Leu Thr Asp Asn Thr
370 375 380Ala Pro Pro Ser Gly Phe Glu Ala Phe Val His Asp Trp Lys Thr Gln385 390 395 400Asp Pro Asp Ile Glu Ala Met Arg Ala Val Asn Pro Leu Phe Ile Pro
405 410 415Arg Asn His Leu Val Glu Ala Ala Leu Ala Asp Ala Val Glu Gly Asn
420 425 430Leu Glu Lys Phe His Glu Leu Leu Ala Ala Val Thr Asn Pro Phe Asp
435 440 445Pro Thr Ala Gly Pro Asp Glu Leu Arg Leu Pro Ser Glu Glu Gly Phe
450 455 460Glu Glu Asp Tyr Met Thr Phe Cys Gly Thr465 470<210>533<211>1236<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1213)<223>RXC01703<400>533gttagacaaa tgggtaaaca gagctgacct agcggaatcc gccatcaacg aaaggcattc 60cgcgagggtt tggggtctgc ctcgaacaaa tcttgggttt gtg gca tgg cca tcc 115
Val Ala Trp Pro Ser
1 5aac gcc aaa gaa aaa ctg ttt atc cac tgg cac tac tgg tgg caa gcg 163Asn Ala Lys Glu Lys Leu Phe Ile His Trp His Tyr Trp Trp Gln Ala
10 15 20cat tat cta gac tgc ctg gtg gat gct gct cgt cga cgc acc aca aag 211His Tyr Leu Asp Cys Leu Val Asp Ala Ala Arg Arg Arg Thr Thr Lys
25 30 35gcc cgt cgc gac cgc atc agg gac acc atc cgc ggc att tcg gtg cgc 259Ala Arg Arg Asp Arg Ile Arg Asp Thr Ile Arg Gly Ile Ser Val Arg
40 45 50aat gtg ggc aag ctg acc tcg aat cgt tat tac gac gac aaa gct tgg 307Asn Val Gly Lys Leu Thr Ser Asn Arg Tyr Tyr Asp Asp Lys Ala Trp
55 60 65ctg gcc ctt gct ctt ggg cgt gcc gga aaa gtg cga aag gtg cgc aca 355Leu Ala Leu Ala Leu Gly Arg Ala Gly Lys Val Arg Lys Val Arg Thr70 75 80 85cca aaa tca ttg ccc tcg ttg gaa caa aac atc gtc gat ggc att gat 403Pro Lys Ser Leu Pro Ser Leu Glu Gln Asn Ile Val Asp Gly Ile Asp
90 95 l00tcc ctt act ggt gtg ctg ccg tgg cgt tcc ggc gaa acc ttc tac aac 451Ser Leu Thr Gly Val Leu Pro Trp Arg Ser Gly Glu Thr Phe Tyr Asn
105 110 115gtt ccc tcc aac ggt cct gct gcg atc atg atg gcc cgc acc gac cgt 499Val Pro Ser Asn Gly Pro Ala Ala Ile Met Met Ala Arg Thr Asp Arg
120 125 130ttg gac gag gct atg aaa atc acc gat tgg att ttt gac aac ctg atc 547Leu Asp Glu Ala Met Lys Ile Thr Asp Trp Ile Phe Asp Asn Leu Ile
135 140 145gat ggc gac ggc ctt gtg atg gac gga ttg cgc atg cgc atg cac gga 595Asp Gly Asp Gly Leu Val Met Asp Gly Leu Arg Met Arg Met His Gly150 155 160 165cct gag ctt gtc cgt tcc atc cac ccg tat tgc caa ggt gtc gcc att 643Pro Glu Leu Val Arg Ser Ile His Pro Tyr Cys Gln Gly Val Ala Ile
170 175 180ggt gcg tgt ttg gaa att gct ctc aaa ctg cgt gag cgc gca ggc ttg 691Gly Ala Cys Leu Glu Ile Ala Leu Lys Leu Arg Glu Arg Ala Gly Leu
185 190 195acc act act gtg gtg gat cac tgg tcg gat gcc gat aag gca gaa gac 739Thr Thr Thr Val Val Asp His Trp Ser Asp Ala Asp Lys Ala Glu Asp
200 205 210tcc ctc aaa tac ttt gca cac atc cac gct gtg gtt cag gct gtg tcg 787Ser Leu Lys Tyr Phe Ala His Ile His Ala Val Val Gln Ala Val Ser
215 220 225cgg aag atg acc aac ttc cac ggc gtt att gat tgg gac acc ggt gac 835Arg Lys Met Thr Asn Phe His Gly Val Ile Asp Trp Asp Thr Gly Asp230 235 240 245ggc gac ggc ggt ttg ttc aag ggc att ttg gtc cgc tat tta gct gat 883Gly Asp Gly Gly Leu Phe Lys Gly Ile Leu Val Arg Tyr Leu Ala Asp
250 255 260gtg gcc atc cgc ctg cct gac gat tca cca acc aac cgg gaa acc aaa 931Val Ala Ile Arg Leu Pro Asp Asp Ser Pro Thr Asn Arg Glu Thr Lys
265 270 275aag att gca gca cgc ctg gta ctg gaa tcg gcg gaa agc gta tgg aac 979Lys Ile Ala Ala Arg Leu Val Leu Glu Ser Ala Glu Ser Val Trp Asn
280 285 290cac cga ttg gaa gtt gat ggc ctt ccg gta ttc gcc aca gac tgg aca 1027His Arg Leu Glu Val Asp Gly Leu Pro Val Phe Ala Thr Asp Trp Thr
295 300 305acg gat gca cgc ctg cca caa aac ttt ggt ttg agt tcc tct agt ttg 1075Thr Asp Ala Arg Leu Pro Gln Asn Phe Gly Leu Ser Ser Ser Ser Leu310 315 320 325agc gat ctg gtg agt gtt gtg cgc gtg gat gaa cgt gat ctg tcc gtg 1123Ser Asp Leu Val Ser Val Val Arg Val Asp Glu Arg Asp Leu Ser Val
330 335 340caa ttg tcc ggt tgg atg ctc atg gaa gca gca gcg aaa gtg gcc gaa 1171Gln Leu Ser Gly Trp Met Leu Met Glu Ala Ala Ala Lys Val Ala Glu
345 350 355gaa ctg gaa aac aac ggc aat agt tac acc ggt cgc tcc cga 1213Glu Leu Glu Asn Asn Gly Asn Ser Tyr Thr Gly Arg Ser Arg
360 365 370tagccccgat agtgtatgtg ctg 1236<210>534<211>371<212>PRT<213>谷氨酸棒杆菌<400>534Val Ala Trp Pro Ser Asn Ala Lys Glu Lys Leu Phe Ile His Trp His1 5 10 15Tyr Trp Trp Gln Ala His Tyr Leu Asp Cys Leu Val Asp Ala Ala Arg
20 25 30Arg Arg Thr Thr Lys Ala Arg Arg Asp Arg Ile Arg Asp Thr Ile Arg
35 40 45Gly Ile Ser Val Arg Asn Val Gly Lys Leu Thr Ser Asn Arg Tyr Tyr
50 55 60Asp Asp Lys Ala Trp Leu Ala Leu Ala Leu Gly Arg Ala Gly Lys Val65 70 75 80Arg Lys Val Arg Thr Pro Lys Ser Leu Pro Ser Leu Glu Gln Asn Ile
85 90 95Val Asp Gly Ile Asp Ser Leu Thr Gly Val Leu Pro Trp Arg Ser Gly
100 105 110Glu Thr Phe Tyr Asn Val Pro Ser Asn Gly Pro Ala Ala Ile Met Met
115 120 125Ala Arg Thr Asp Arg Leu Asp Glu Ala Met Lys Ile Thr Asp Trp Ile
130 135 140Phe Asp Asn Leu Ile Asp Gly Asp Gly Leu Val Met Asp Gly Leu Arg145 150 155 160Met Arg Met His Gly Pro Glu Leu Val Arg Ser Ile His Pro Tyr Cys
165 170 175Gln Gly Val Ala Ile Gly Ala Cys Leu Glu Ile Ala Leu Lys Leu Arg
180 185 190Glu Arg Ala Gly Leu Thr Thr Thr Val Val Asp His Trp Ser Asp Ala
195 200 205Asp Lys Ala Glu Asp Ser Leu Lys Tyr Phe Ala His Ile His Ala Val
210 215 220Val Gln Ala Val Ser Arg Lys Met Thr Asn Phe His Gly Val Ile Asp225 230 235 240Trp Asp Thr Gly Asp Gly Asp Gly Gly Leu Phe Lys Gly Ile Leu Val
245 250 255Arg Tyr Leu Ala Asp Val Ala Ile Arg Leu Pro Asp Asp Ser Pro Thr
260 265 270Asn Arg Glu Thr Lys Lys Ile Ala Ala Arg Leu Val Leu Glu Ser Ala
275 280 285Glu Ser Val Trp Asn His Arg Leu Glu Val Asp Gly Leu Pro Val Phe
290 295 300Ala Thr Asp Trp Thr Thr Asp Ala Arg Leu Pro Gln Asn Phe Gly Leu305 310 315 320Ser Ser Ser Ser Leu Ser Asp Leu Val Ser Val Val Arg Val Asp Glu
325 330 335Arg Asp Leu Ser Val Gln Leu Ser Gly Trp Met Leu Met Glu Ala Ala
340 345 350Ala Lys Val Ala Glu Glu Leu Glu Asn Asn Gly Asn Ser Tyr Thr Gly
355 360 365Arg Ser Arg
370<210>535<211>800<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(777)<223>RXC02254<400>535atc gcc gtt gcc gaa gaa ggc gga ttg tgg gaa aac ctc ctg cag cac 48Ile Ala Val Ala Glu Glu Gly Gly Leu Trp Glu Asn Leu Leu Gln His1 5 10 15cgc ttc ggt gga cat ggt gcg cta gct ggt cac gcc ttg gga aac ctc 96Arg Phe Gly Gly His Gly Ala Leu Ala Gly His Ala Leu Gly Asn Leu
20 25 30gtg atc gcg gcg ttg acc gac att ttg ggc acc tcc cag cat gcg ctt 144Val Ile Ala Ala Leu Thr Asp Ile Leu Gly Thr Ser Gln His Ala Leu
35 40 45gat caa atc gct caa ctc gct gga gcc aaa gga cgc atc atc ccg gta 192Asp Gln Ile Ala Gln Leu Ala Gly Ala Lys Gly Arg Ile Ile Pro Val
50 55 60tgt gct gaa cct ttg gat ctt gaa gcg gaa gta tca ggt cta gac tct 240Cys Ala Glu Pro Leu Asp Leu Glu Ala Glu Val Ser Gly Leu Asp Ser65 70 75 80gat gct cga gtc atg cgt caa gtt cgt ggt caa gtg gcg gta gct gca 288Asp Ala Arg Val Met Arg Gln Val Arg Gly Gln Val Ala Val Ala Ala
85 90 95acc ccc ggg cag gtg cga cgc gtt cga atc att ccg gac aat cca gaa 336Thr Pro Gly Gln Val Arg Arg Val Arg Ile Ile Pro Asp Asn Pro Glu
100 105 110ccg aac ccc gct gcc atc gag gcc att ctc gat gca gat ttg gtc acc 384Pro Asn Pro Ala Als Ile Glu Ala Ile Leu Asp Ala Asp Leu Val Thr
115 120 125ctt ggc cca ggt tcc tgg ttc tcc tct gtg att cca cac att ttg gtc 432Leu Gly Pro Gly Ser Trp Phe Ser Ser Val Ile Pro His Ile Leu Val
130 135 140cca ggg atc gtt gat gcc ttg gcg cag aca aaa gca acc aaa acc gtg 480Pro Gly Ile Val Asp Ala Leu Ala Gln Thr Lys Ala Thr Lys Thr Val145 150 155 160gtg tta aac ctg acg tcc gag cca ggg gag acc gcg gga ttc tct gca 528Val Leu Asn Leu Thr Ser Glu Pro Gly Glu Thr Ala Gly Phe Ser Ala
165 170 175gaa cga cac atc cat gtg ctc cgc cag cat gct cga aac ctt cag gtt 576Glu Arg His Ile His Val Leu Arg Gln His Ala Arg Asn Leu Gln Val
180 185 190gac caa gtc att gtc gat gcc aag aca ctg tcc tca caa acc gaa cgc 624Asp Gln Val Ile Val Asp Ala Lys Thr Leu Ser Ser Gln Thr Glu Arg
195 200 205aat cat gta gaa cga gct gct cgc acc ctt ggt gca gaa gtc tcc ttc 672Asn His Val Glu Arg Ala Ala Arg Thr Leu Gly Ala Glu Val Ser Phe
210 215 220cat gat gtc cag gct gaa gat ggc cgt ggt cga ttc acc agt att cac 720His Asp Val Gln Ala Glu Asp Gly Arg Gly Arg Phe Thr Ser Ile His225 230 235 240gat cca gca aag ctg tgt gca gcg ttg ctg gca agt ttt gct gga gca 768Asp Pro Ala Lys Leu Cys Ala Ala Leu Leu Ala Ser Phe Ala Gly Ala
245 250 255cga aag cgt taaggagtag gcgtgtcact gac 800Arg Lys Arg<210>536<211>259<212>PRT<213>谷氨酸棒杆菌<400>536Ile Ala Val Ala Glu Glu Gly Gly Leu Trp Glu Asn Leu Leu Gln His1 5 10 15Arg Phe Gly Gly His Gly Ala Leu Ala Gly His Ala Leu Gly Asn Leu
20 25 30Val Ile Ala Ala Leu Thr Asp Ile Leu Gly Thr Ser Gln His Ala Leu
35 40 45Asp Gln Ile Ala Gln Leu Ala Gly Ala Lys Gly Arg Ile Ile Pro Val
50 55 60Cys Ala Glu Pro Leu Asp Leu Glu Ala Glu Val Ser Gly Leu Asp Ser65 70 75 80Asp Ala Arg Val Met Arg Gln Val Arg Gly Gln Val Ala Val Ala Ala
85 90 95Thr Pro Gly Gln Val Arg Arg Val Arg Ile Ile Pro Asp Asn Pro Glu
100 105 110Pro Asn Pro Ala Ala Ile Glu Ala Ile Leu Asp Ala Asp Leu Val Thr
115 120 125Leu Gly Pro Gly Ser Trp Phe Ser Ser Val Ile Pro His Ile Leu Val
130 135 140Pro Gly Ile Val Asp Ala Leu Ala Gln Thr Lys Ala Thr Lys Thr Val145 150 155 160Val Leu Asn Leu Thr Ser Glu Pro Gly Glu Thr Ala Gly Phe Ser Ala
165 170 175Glu Arg His Ile His Val Leu Arg Gln His Ala Arg Asn Leu Gln Val
180 185 190Asp Gln Val Ile Val Asp Ala Lys Thr Leu Ser Ser Gln Thr Glu Arg
195 200 205Asn His Val Glu Arg Ala Ala Arg Thr Leu Gly Ala Glu Val Ser Phe
210 215 220His Asp Val Gln Ala Glu Asp Gly Arg Gly Arg Phe Thr Ser Ile His225 230 235 240Asp Pro Ala Lys Leu Cys Ala Ala Leu Leu Ala Ser Phe Ala Gly Ala
245 250 255Arg Lys Arg<210>537<211>1059<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1036)<223>RXC02255<400>537gtgtgcagcg ttgctggcaa gttttgctgg agcacgaaag cgttaaggag taggcgtgtc 60actgacgagt gatatcaaac aagaattggc gcaggtccat gtg gcc aaa aac agt 115
Val Ala Lys Asn Ser
1 5
gtt cgt gct gcg gaa gtg tct gca att tta agg ttt gct ggt gag atg 163Val Arg Ala Ala Glu Val Ser Ala Ile Leu Arg Phe Ala Gly Glu Met
10 15 20caa gct gtc ggc ggc aag ctg gtc atc gaa gca aat ttg gac agc atg 211Gln Ala Val Gly Gly Lys Leu Val Ile Glu Ala Asn Leu Asp Ser Met
25 30 35caa gtc ggt atg agg ctt cag gag ttt atc caa ggt ttg tac aac tct 259Gln Val Gly Met Arg Leu Gln Glu Phe Ile Gln Gly Leu Tyr Asn Ser
40 45 50cga gtc gat gtg cac acc gtg aac ccg act gtg agc agg aaa acg cca 307Arg VAl Asp Val His Thr Val Asn Pro Thr Val Ser Arg Lys Thr Pro
55 60 65cgg tat ttg gtg cgc atc att gac aat gcc gat gaa att gcg cga cgc 355Arg Tyr Leu Val Arg Ile Ile Asp Asn Ala Asp Glu Ile Ala Arg Arg70 75 80 85acc gga ctg gtc acc agg tct gga cat gtg gtt aaa ggt cta gcg cct 403Thr Gly Leu Val Thr Arg Ser Gly His Val Val Lys Gly Leu Ala Pro
90 95 100tct gtg gtc agc gga aca atc agt gac gct gaa gct gca tgg cgc ggt 451Ser Val Val Ser Gly Thr Ile Ser Asp Ala Glu Ala Ala Trp Arg Gly
105 110 115gcg ttt cta gcc aat gga tct tta agt gat cca ggt cgt tcc tct tcg 499Ala Phe Leu Ala Asn Gly Ser Leu Ser Asp Pro Gly Arg Ser Ser Ser
120 125 130ttg gag gtg ttg tgt cct ggt caa gaa tca gca ttg gca ctg gtt gga 547Leu Glu Val Leu Cys Pro Gly Gln Glu Ser Ala Leu Ala Leu Val Gly
135 140 145tgt gcg cga aga att ggg atc gcg gcg aaa acg aaa gat tct cga gga 595Cys Ala Arg Arg Ile Gly Ile Ala Ala Lys Thr Lys Asp Ser Arg Gly150 155 160 165ttt gat cgc gtc aat gtt cgt gat gcg gaa gca att ggg gca ctg ctc 543Phe Asp Arg Val Asn Val Arg Asp Ala Glu Ala Ile Gly Ala Leu Leu
170 175 180act cga atg ggt gcc cag aaa act cgc atg ttg tgg gaa gaa aaa cgc 691Thr Arg Met Gly Ala Gln Lys Thr Arg Met Leu Trp Glu Glu Lys Arg
185 190 195atc aag cgg gaa agt cga act ccg caa acc ggg ttg gcc aac ttc gac 739Ile Lys Arg Glu Ser Arg Thr Pro Gln Thr Gly Leu Ala Asn Phe Asp
200 205 210gat gcc aat ctg cgc agg tca gcc cga gca gca gtt gcc gct gca gcg 787Asp Ala Asn Leu Arg Arg Ser Ala Arg Ala Ala Val Ala Ala Ala Ala
215 220 225agg gta gaa cgc gcc atg aag att ctt ggt gat gat gtt cct gag cat 835Arg Val Glu Arg Ala Met Lys Ile Leu Gly Asp Asp Val Pro Glu His230 235 240 245ttg gct gag gct gga cag ctg cgt gtg cag cac cgt cag gca tcg ttg 883Leu Ala Glu Ala Gly Gln Leu Arg Val Gln His Arg Gln Ala Ser Leu
250 255 260gag gag ttg ggc cgg ttg gct gat cct caa atg acc aag gat gct gtg 931Glu Glu Leu Gly Arg Leu Ala Asp Pro Gln Met Thr Lys Asp Ala Val
265 270 275gcc ggt cgt att cgt cgt ctt ttg acg atg gca gat aag cgc gcc gaa 979Ala Gly Arg Ile Arg Arg Leu Leu Thr Met Ala Asp Lys Arg Ala Glu
280 285 290gat ctg aag att cct gat aca aat tct gtt gtg acg gaa gat ttg ttg 1027Asp Leu Lys Ile Pro Asp Thr Asn Ser Val Val Thr Glu Asp Leu Leu
295 300 305gaa gaa att tagatgattg aagcctaaaa acg 1059Glu Glu Ile310<210>538<211>312<212>PRT<213>谷氨酸棒杆菌<400>538Val Ala Lys Asn Ser Val Arg Ala Ala Glu Val Ser Ala Ile Leu Arg1 5 10 15Phe Ala Gly Glu Met Gln Ala Val Gly Gly Lys Leu Val Ile Glu Ala
20 25 30Asn Leu Asp Ser Met Gln Val Gly Met Arg Leu Gln Glu Phe Ile Gln
35 40 45Gly Leu Tyr Asn Ser Arg Val Asp Val His Thr Val Asn Pro Thr Val
50 55 60Ser Arg Lys Thr Pro Arg Tyr Leu Val Arg Ile Ile Asp Asn Ala Asp65 70 75 80Glu Ile Ala Arg Arg Thr Gly Leu Val Thr Arg Ser Gly His Val Val
85 90 95Lys Gly Leu Ala Pro Ser Val Val Ser Gly Thr Ile Ser Asp Ala Glu
100 105 110Ala Ala Trp Arg Gly Ala Phe Leu Ala Asn Gly Ser Leu Ser Asp Pro
115 120 125Gly Arg Ser Ser Ser Leu Glu Val Leu Cys Pro Gly Gln Glu Ser Ala
130 135 140Leu Ala Leu Val Gly Cys Ala Arg Arg Ile Gly Ile Ala Ala Lys Thr145 150 155 160Lys Asp Ser Arg Gly Phe Asp Arg Val Asn Val Arg Asp Ala Glu Ala
165 170 175Ile Gly Ala Leu Leu Thr Arg Met Gly Ala Gln Lys Thr Arg Met Leu
180 185 190Trp Glu Glu Lys Arg Ile Lys Arg Glu Ser Arg Thr Pro Gln Thr Gly
195 200 205Leu Ala Asn Phe Asp Asp Ala Asn Leu Arg Arg Ser Ala Arg Ala Ala
210 215 220Val Ala Ala Ala Ala Arg Val Glu Arg Ala Met Lys Ile Leu Gly Asp225 230 235 240Asp Val Pro Glu His Leu Ala Glu Ala Gly Gln Leu Arg Val Gln His
245 250 255Arg Gln Ala Ser Leu Glu Glu Leu Gly Arg Leu Ala Asp Pro Gln Met
260 265 270Thr Lys Asp Ala Val Ala Gly Arg Ile Arg Arg Leu Leu Thr Met Ala
275 280 285Asp Lys Arg Ala Glu Asp Leu Lys Ile Pro Asp Thr Asn Ser Val Val
290 295 300Thr Glu Asp Leu Leu Glu Glu Ile305 310<210>539<211>681<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(658)<223>RXC02435<400>539tcatgaatgt agaacggatt tatcgacaag cgccagcaag tacgttgatc tctttagcca 60tcatcgctgt gtacgcagtg cggccattca atcgagatca gtg aca gac aac ctg 115
Val Thr Asp Asn Leu
1 5ggt tca acc agt atc ggt gac gcg tgg att ctg tac gca ccg ctg atg 163Gly Ser Thr Ser Ile Gly Asp Ala Trp Ile Leu Tyr Ala Pro Leu Met
10 15 20gat gat ggt ggc ttt ggt cca ctg cgt gcc atc gga gga atg ttc ctg 211Asp Asp Gly Gly Phe Gly Pro Leu Arg Ala Ile Gly Gly Met Phe Leu
25 30 35cac att ggc ccc ggg cac atg ctg ttg aac ctt gtg ttg ttg tgg ttg 259His Ile Gly Pro Gly His Met Leu Leu Asn Leu Val Leu Leu Trp Leu
40 45 50ctg gga aga gaa att gaa cga gac ttc ggt tct gcg ctt ttc act gcg 307Leu Gly Arg Glu Ile Glu Arg Asp Phe Gly Ser Ala Leu Phe Thr Ala
55 60 65atg tac ttt gtg ggc ggt att ggt gcg tct gca gct gtc atc tgg atg 355Met Tyr Phe Val Gly Gly Ile Gly Ala Ser Ala Ala Val Ile Trp Met70 75 80 85gat ccc tat tca ccg aca gca ggt gct tcc ggc gcc att tac gcc atg 403Asp Pro Tyr Ser Pro Thr Ala Gly Ala Ser Gly Ala Ile Tyr Ala Met
90 95 100atg gct att ttg gtg ggg ctt ttt gtg tta aga agc gcg gat atc cga 451Met Ala Ile Leu Val Gly Leu Phe Val Leu Arg Ser Ala Asp Ile Arg
105 110 115gca ccc ttg atc ctt atc gcc atc aac atc gcc tat acc ttg atg tcc 499Ala Pro Leu Ile Leu Ile Ala Ile Asn Ile Ala Tyr Thr Leu Met Ser
120 125 130acc aat gtt tct ctg tgg gga cac ctt gga ggt ttg atc act gga gct 547Thr Asn Val Ser Leu Trp Gly His Leu Gly Gly Leu Ile Thr Gly Ala
135 140 145tta att act tgg cca atg gtt aaa gcg aaa act caa aga aca cgg tgg 595Leu Ile Thr Trp Pro Met Val Lys Ala Lys Thr Gln Arg Thr Arg Trp150 155 160 165att atc gtg ctc att ggt ttt gct gta gtt gtg gct gct gtc att cta 643Ile Ile Val Leu Ile Gly Phe Ala Val Val Val Ala Ala Val Ile Leu
170 175 180gga att gac cgg gtg tagacacatt ccgcccattg ccc 681Gly Ile Asp Arg Val
185<2l0>540<211>186<212>PRT<213>谷氨酸棒杆菌<400>540Val Thr Asp Asn Leu Gly Ser Thr Ser Ile Gly Asp Ala Trp Ile Leu1 5 10 15Tyr Ala Pro Leu Met Asp Asp Gly Gly Phe Gly Pro Leu Arg Ala Ile
20 25 30Gly Gly Meu Phe Leu His Ile Gly Pro Gly His Met Leu Leu Asn Leu
35 40 45Val Leu Leu Trp Leu Leu Gly Arg Glu Ile Glu Arg Asp Phe Gly Ser
50 55 60Ala Leu Phe Thr Ala Met Tyr Phe Val Gly Gly Ile Gly Ala Ser Ala65 70 75 80Ala Val Ile Trp Met Asp Pro Tyr Ser Pro Thr Ala Gly Ala Ser Gly
85 90 95Ala Ile Tyr Ala Met Met Ala Ile Leu Val Gly Leu Phe Val Leu Arg
100 105 110Ser Ala Asp Ile Arg Ala Prp Leu Ile Leu Ile Ala Ile Asn Ile Ala
115 120 125Tyr Tyr Leu Met Ser Thr Asn Val Ser Leu Trp Gly His Leu Gly Gly
130 135 140Leu Ile Thr Gly Ala Leu Ile Thr Trp Pro Met Val Lys Ala Lys Thr145 150 155 160Gln Arg Thr Arg Trp Ile Ile Val Leu Ile Gly Phe Ala Val Val Val
165 170 175Ala Ala Val Ile Leu Gly Ile Asp Arg Val
180 185<210>541<211>681<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(658)<223>FRXA02435<400>541tcatgaatgt agaacggatt tatcgacaag cgccagcaag tacgttgatc tctttagcca 60tcatcgctgt gtacgcagtg cggccattca atcgagatca gtg aca gac aac ctg 115
Val Thr Asp Asn Leu
1 5ggt tca acc agt atc ggt gac gcg tgg att ctg tac gca ccg ctg atg 163Gly Ser Thr Ser Ile Gly Asp Ala Trp Ile Leu Tyr Ala Pro Leu Met
10 15 20gat gat ggt ggc ttt ggt cca ctg cgt gcc atc gga gga atg ttc ctg 211Asp Asp Gly Gly Phe Gly Pro Leu Arg Ala Ile Gly Gly Met Phe Leu
25 30 35cac att ggc ccc ggg cac atg ctg ttg aac ctt gtg ttg ttg tgg ttg 259His Ile Gly Pro Gly His Met Leu Leu Asn Leu Val Leu Leu Trp Leu
40 45 50ctg gga aga gaa att gaa cga gac ttc ggt tct gcg ctt ttc act gcg 307Leu Gly Arg Glu Ile Glu Arg Asp Phe Gly Ser Ala Leu Phe Thr Ala
55 60 65atg tac ttt gtg ggc ggt att ggt gcg tct gca gct gtc atc tgg atg 355Met Tyr Phe Val Gly Gly Ile Gly Ala Ser Ala Ala Val Ile Trp Met70 75 80 85gat ccc tat tca ccg aca gca ggt gct tcc ggc gcc att tac gcc atg 403Asp Pro Tyr Ser Pro Thr Ala Gly Ala Ser Gly Ala Ile Tyr Ala Met
90 95 100atg gct att ttg gtg ggg ctt ttt gtg tta aga agc gcg gat atc cga 451Met Ala Ile Leu Val Gly Leu Phe Val Leu Arg Ser Ala Asp Ile Arg
105 110 115gca ccc ttg atc ctt atc gcc atc aac atc gcc tat acc ttg atg tcc 499Ala Pro Leu Ile Leu Ile Ala Ile Asn Ile Ala Tyr Thr Leu Met Ser
120 125 130acc aat gtt tct ctg tgg gga cac ctt gga ggt ttg atc act gga gct 547Thr Asn Val Ser Leu Trp Gly His Leu Gly Gly Leu Ile Thr Gly Ala
135 140 145tta att act tgg cca atg gtt aaa gcg aaa act caa aga aca cgg tgg 595Leu Ile Thr Trp Pro Met Val Lys Ala Lys Thr Gln Arg Thr Arg Trp150 155 160 165att atc gtg ctc att ggt ttt gct gta gtt gtg gct gct gtc att cta 643Ile Ile Val Leu Ile Gly Phe Ala Val Val Val Ala Ala Val Ile Leu
170 175 180gga att gac cgg gtg tagacacatt ccgcccattg ccc 681Gly Ile Asp Arg Val
185<210>542<211>186<212>PRT<213>谷氨酸棒杆菌<400>542Val Thr Asp Asn Leu Gly Ser Thr Ser Ile Gly Asp Ala Trp Ile Leu1 5 10 15Tyr Ala Pro Leu Met Asp Asp Gly Gly Phe Gly Pro Leu Arg Ala Ile
20 25 30Gly Gly Met Phe Leu His Ile Gly Pro Gly His Met Leu Leu Asn Leu
35 40 45Val Leu Leu Trp Leu Leu Gly Arg Glu Ile Glu Arg Asp Phe Gly Ser
50 55 60Ala Leu Phe Thr Ala Met Tyr Phe Val Gly Gly Ile Gly Ala Ser Ala65 70 75 80Ala Val Ile Trp Met Asp Pro Tyr Ser Pro Thr Ala Gly Ala Ser Gly
85 90 95Ala Ile Tyr Ala Met Met Ala Ile Leu Val Gly Leu Phe Val Leu Arg
100 105 110Ser Ala Asp Ile Arg Ala Pro Leu Ile Leu Ile Ala Ile Asn Ile Ala
115 120 125Tyr Thr Leu Met Ser Thr Asn Val Ser Leu Trp Gly His Leu Gly Gly
130 135 140Leu Ile Thr Gly Ala Leu Ile Thr Trp Pro Met Val Lys Ala Lys Thr145 150 155 160Gln Arg Thr Arg Trp Ile Ile Val Leu Ile Gly Phe Ala Val Val Val
165 170 175Ala Ala Val Ile Leu Gly Ile Asp Arg Val
180 185<210>543<211>1141<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1111)<223>RXC03216<400>543gctgtatcac agtcagctgc agcaggtggc gaaaccgttg cggtcccagc ggctgctctg 60atccctgcaa acaactagaa actattcaga aagcatcacc atg aaa ctc ggt ctc 115
Met Lys Leu Gly Leu
1 5tac aac gcg atc ttc cac gac cgc acc ctg cca gaa gcg ctc gca gcc 163Tyr Asn Ala Ile Phe His Asp Arg Thr Leu Pro Glu Ala Leu Ala Ala
10 15 20atc aaa gct gca ggt ctc acc gga att gaa ctc aac acc ggc gga ttt 211Ile Lys Ala Ala Gly Leu Thr Gly Ile Glu Leu Asn Thr Gly Gly Phe
25 30 35ttg cct gca acc cac atc ccg acc atc gat gac atc ctg gtc agc gat 259Leu Pro Ala Thr His Ile Pro Thr Ile Asp Asp Ile Leu Val Ser Asp
40 45 50gat gcc cgc gat gaa ttc ctc ggg att ttc gaa ggc acc ggc gtc gac 307Asp Ala Arg Asp Glu Phe Leu Gly Ile Phe Glu Gly Thr Gly Val Asp
55 60 65atc tac ggc ctt aac tgc aac ggc aac ccg ctt cac ccc aac aag gcg 355Ile Tyr Gly Leu Asn Cys Asn Gly Asn Pro Leu His Pro Asn Lys Ala70 75 80 85atc ggg gac aag cat gcc gaa gac att cga cgt tcc atc cgc ctc gca 403Ile Gly Asp Lys His Ala Glu Asp Ile Arg Arg Ser Ile Arg Leu Ala
90 95 100gag cgc ctc ggc caa aac cgt gtg gtc acc atg tct ggt ctt cct ggt 451Glu Arg Leu Gly Gln Asn Arg Val Val Thr Met Ser Gly Leu Pro Gly
105 110 115ggc gaa cca ggc gcg aag tac acc aac tgg gtt gtc aac gcg tgg aac 499Gly Glu Pro Gly Ala Lys Tyr Thr Asn Trp Val Val Asn Ala Trp Asn
120 125 130tcc gca gcc ttg gat gtc ctt gat tac caa tgg gat atc gca gct gaa 547Ser Ala Ala Leu Asp Val Leu Asp Tyr Gln Trp Asp Ile Ala Ala Glu
135 140 145ttc tgg cgc gag acc gac cgc ttc gcc gca gat cac ggc gtg aaa gtg 595Phe Trp Arg Glu Thr Asp Arg Phe Ala Ala Asp His Gly Val Lys Val150 155 160 165gct ctt gag ctg cac cca cag aac atc gtg ttc aac tcc gct gac gtg 643Ala Leu Glu Leu His Pro Gln Asn Ile Val Phe Asn Ser Ala Asp Val
170 175 180cat aag ctc atc gat ctc acc ggc gcc acc cac gtg ggc gtc gaa ctg 691His Lys Leu Ile Asp Leu Thr Gly Ala Thr His Val Gly Val Glu Leu
185 190 195gat gca tca cac ctg ttc tgg cag cag atg gac cca atc gct gtg att 739Asp Ala Ser His Leu Phe Trp Gln Gln Met Asp Pro Ile Ala Val Ile
200 205 210gat cac ctc ggc gag ctc atc ttc cac gcc gcc gcc aaa gac gtg cga 787Asp His Leu Gly Glu Leu Ile Phe His Ala Ala Ala Lys Asp Val Arg
215 220 225gtt aat aag gaa tgg gct cag ctc aac ggt gtg ctg gac aac agc ttc 835Val Asn Lys Glu Trp Ala Gln Leu Asn Gly Val Leu Asp Asn Ser Phe230 235 240 245cga cgc ctt gac cca tcc gaa aac cgc acc aac ttg ggc ggc gac gag 883Arg Arg Leu Asp Pro Ser Glu Asn Arg Thr Asn Leu Gly Gly Asp Glu
250 255 260tgg gcg aat gaa tgg cca aag aac tct gct tgg gat ttc gtt gct ctg 931Trp Ala Asn Glu Trp Pro Lys Asn Ser Ala Trp Asp Phe Val Ala Leu
265 270 275ggc cgc ggt cat gac gtt gct tac tgg acc gaa ttc ctc cgc gca ctt 979Gly Arg Gly His Asp Val Ala Tyr Trp Thr Glu Phe Leu Arg Ala Leu
280 285 290cac cgc gtc gat cca aac atg ctg gtc aac atc gaa cac gag gat gtt 1027His Arg Val Asp Pro Asn Met Leu Val Asn Ile Glu His Glu Asp Val
295 300 305tca ctc ggt cgc gaa gaa ggc gtc aac gaa gcc gct aag gtg ctg atc 1075Ser Leu Gly Arg Glu Glu Gly Val Asn Glu Ala Ala Lys Val Leu Ile310 315 320 325gag gcc aac aag gca ctc gaa gag tcc ctg gtt tct taaaaaaact 1121Glu Ala Asn Lys Ala Leu Glu Glu Ser Leu Val Ser
330 335acgcctgccc cgcaacgctt 1141<210>544<211>337<212>PRT<213>谷氨酸棒杆菌Met Lys Leu Gly Leu Tyr Asn Ala Ile Phe His Asp Arg Thr Leu Pro1 5 10 15Glu Ala Leu Ala Ala Ile Lys Ala Ala Gly Leu Thr Gly Ile Glu Leu
20 25 30Asn Thr Gly Gly Phe Leu Pro Ala Thr His Ile Pro Thr Ile Asp Asp
35 40 45Ile Leu Val Ser Asp Asp Ala Arg Asp Glu Phe Leu Gly Ile Phe Glu
50 55 60Gly Thr Gly Val Asp Ile Tyr Gly Leu Asn Cys Asn Gly Asn Pro Leu65 70 75 80His Pro Asn Lys Ala Ile Gly Asp Lys His Ala Glu Asp Ile Arg Arg
85 90 95Ser Ile Arg Leu Ala Glu Arg Leu Gln Gln Asn Arg Val Val Thr Met
100 105 110Ser Gly Leu Pro Gly Gly Glu Pro Gly Ala Lys Tyr Thr Asn Trp Val
115 120 125Val Asn Ala Trp Asn Ser Ala Ala Leu Asp Val Leu Asp Tyr Gln Trp
130 135 140Asp Ile Ala Ala Glu Phe Trp Arg Glu Thr Asp Arg Phe Ala Ala Asp145 150 155 160His Gly Val Lys Val Ala Leu Glu Leu His Pro Gln Asn Ile Val Phe
165 170 175Asn Ser Ala Asp Val His Lys Leu Ile Asp Leu Thr Gly Ala Thr His
180 185 190Val Gly Val Glu Leu Asp Ala Ser His Leu Phe Trp Gln Gln Met Asp
195 200 205Pro Ile Ala Val Ile Asp His Leu Gly Glu Leu Ile Phe His Ala Ala
210 215 220Ala Lys Asp Val Arg Val Asn Lys Glu Trp Ala Gln Leu Asn Gly Val225 230 235 240Leu Asp Asn Ser Phe Arg Arg Leu Asp Pro Ser Glu Asn Arg Thr Asn
245 250 255Leu Gly Gly Asp Glu Trp Ala Asn Glu Trp Pro Lys Asn Ser Ala Trp
260 265 270Asp Phe Val Ala Leu Gly Arg Gly His Asp Val Ala Tyr Trp Thr Glu
275 280 285Phe Leu Arg Ala Leu His Arg Val Asp Pro Asn Met Leu Val Asn Ile
290 295 300Glu His Glu Asp Val Ser Leu Gly Arg Glu Glu Gly Val Asn Glu Ala305 310 315 320Ala Lys Val Leu Ile Glu Ala Asn Lys Ala Leu Glu Glu Ser Leu Val
325 330 335Ser<210>545<211>1416<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1393)<223>RXA02175<400>545tgacatgcgc ttggcgcatc ccagttggta agaataaacg ggactacttc cgtaatccgg 60aagagttttt ttccgaacaa atatgtttga aagggatatc gtg gct act gat aac 115
Val Ala Thr Asp Asn
1 5aac aag gct gtc ctg cac tac ccc ggt ggc gag ttc gaa atg gac atc 163Asn Lys Ala Val Leu His Tyr Pro Gly Gly Glu Phe Glu Met Asp Ile
10 15 20atc gag gct tct gag ggt aac aac ggt gtt gtc ctg ggc aag atg ctg 211Ile Glu Ala Ser Glu Gly Asn Asn Gly Val Val Leu Gly Lys Met Leu
25 30 35tct gag act gga ctg atc act ttt gac cca ggt tat gtg agc act ggc 259Ser Glu Thr Gly Leu Ile Thr Phe Asp Pro Gly Tyr Val Ser Thr Gly
40 45 50tcc acc gag tcg aag atc acc tac atc gat ggc gat gcg gga atc ctg 307Ser Thr Glu Ser Lys Ile Thr Tyr Ile Asp Gly Asp Ala Gly Ile Leu
55 60 65cgt tac cgc ggc tat gac atc gct gat ctg gct gag aat gcc acc ttc 355Arg Tyr Arg Gly Tyr Asp Ile Ala Asp Leu Ala Glu Asn Ala Thr Phe70 75 80 85aac gag gtt tct tac cta ctt atc aac ggt gag cta cca acc cca gat 403Asn Glu Val Ser Tyr Leu Leu Ile Asn Gly Glu Leu Pro Thr Pro Asp
90 95 100gag ctt cac aag ttt aac gac gag att cgc cac cac acc ctt ctg gac 451Glu Leu His Lys Phe Asn Asp Glu Ile Arg His His Thr Leu Leu Asp
105 110 115gag gac ttc aag tcc cag ttc aac gtg ttc cca cgc gac gct cac cca 499Glu Asp Phe Lys Ser Gln Phe Asn Val Phe Pro Arg Asp Ala His Pro
120 125 130atg gca acc ttg gct tcc tcg gtt aac att ttg tct acc tac tac cag 547Met Ala Thr Leu Ala Ser Ser Val Asn Ile Leu Ser Thr Tyr Tyr Gln
135 140 145gac cag ctg aac cca ctc gat gag gca cag ctt gat aag gca acc gtt 595Asp Gln Leu Asn Pro Leu Asp Glu Ala Gln Leu Asp Lys Ala Thr Val150 155 160 165cgc ctc atg gca aag gtt cca atg ctg gct gcg tac gca cac cgc gca 643Arg Leu Met Ala Lys Val Pro Met Leu Ala Ala Tyr Ala His Arg Ala
170 175 180cgc aag ggt gct cct tac atg tac cca gac aac tcc ctc aat gcg cgt 691Arg Lys Gly Ala Pro Tyr Met Tyr Pro Asp Asn Ser Leu Asn Ala Arg
185 190 195gag aac ttc ctg cgc atg atg ttc ggt tac cca acc gag cca tac gag 739Glu Asn Phe Leu Arg Met Met Phe Gly Tyr Pro Thr Glu Pro Tyr Glu
200 205 210atc gac cca atc atg gtc aag gct ctg gac aag ctg ctc atc ctg cac 787Ile Asp Pro Ile Met Val Lys Ala Leu Asp Lys Leu Leu Ile Leu His
215 220 225gct gac cac gag cag aac tgc tcc acc tcc acc gtt cgt atg atc ggt 835Ala Asp His Glu Gln Asn Cys Ser Thr Ser Thr Val Arg Met Ile Gly230 235 240 245tcc gca cag gcc aac atg ttt gtc tcc atc gct ggt ggc atc aac gct 883Ser Ala Gln Ala Asn Met Phe Val Ser Ile Ala Gly Gly Ile Asn Ala
250 255 260ctg tcc ggc cca ctg cac ggt ggc gca aac cag gct gtt ctg gag atg 931Leu Ser Gly Pro Leu His Gly Gly Ala Asn Gln Ala Val Leu Glu Met
265 270 275ctc gaa gac atc aag agc aac cac ggt ggc gac gca acc gag ttc atg 979Leu Glu Asp Ile Lys Ser Asn His Gly Gly Asp Ala Thr Glu Phe Met
280 285 290aac aag gtc aag aac aag gaa gac ggc gtc cgc ctc atg ggc ttc gga 1027Asn Lys Val Lys Asn Lys Glu Asp Gly Val Arg Leu Met Gly Phe Gly
295 300 305cac cgc gtt tac aag aac tac gat cca cgt gca gca atc gtc aag gag 1075His Arg Val Tyr Lys Asn Tyr Asp Pro Arg Ala Ala Ile Val Lys Glu310 315 320 325acc gca cac gag atc ctc gag cac ctc ggt ggc gac gat ctt ctg gat 1123Thr Ala His Glu Ile Leu Glu His Leu Gly Gly Asp Asp Leu Leu Asp
330 335 340ctg gca atc aag ctg gaa gaa att gca ctg gct gat gat tac ttc atc 1171Leu Ala Ile Lys Leu Glu Glu Ile Ala Leu Ala Asp Asp Tyr Phe Ile
345 350 355tcc cgc aag ctc tac ccg aac gta gac ttc tac acc ggc ctg atc tac 1219Ser Arg Lys Leu Tyr Pro Asn Val Asp Phe Tyr Thr Gly Leu Ile Tyr
360 365 370cgc gca atg ggc ttc cca act gac ttc ttc acc gta ttg ttc gca atc 1267Arg Ala Met Gly Phe Pro Thr Asp Phe Phe Thr Val Leu Phe Ala Ile
375 380 385ggt cgt ctg cca gga tgg atc gct cac tac cgc gag cag ctc ggt gca 1315Gly Arg Leu Pro Gly Trp Ile Ala His Tyr Arg Glu Gln Leu Gly Ala390 395 400 405gca ggc aac aag atc aac cgc cca cgc cag gtc tac acc ggc aac gaa 1363Ala Gly Asn Lys Ile Asn Arg Pro Arg Gln Val Tyr Thr Gly Asn Glu
410 415 420tcc cgc aag ttg gtt cct cgc gag gag cgc taaatttagc ggatgattct 1413Ser Arg Lys Leu Val Pro Arg Glu Glu Arg
425 430cgt 1416<210>546<211>431<212>PRT<213>谷氨酸棒杆菌Val Ala Thr Asp Asn Asn Lys Ala Val Leu His Tyr Pro Gly Gly Glu1 5 10 15Phe Glu Met Asp Ile Ile Glu Ala Ser Glu Gly Asn Asn Gly Val Val
20 25 30Leu Gly Lys Met Leu Ser Glu Thr Gly Leu Ile Thr Phe Asp Pro Gly
35 40 45Tyr Val Ser Thr Gly Ser Thr Glu Ser Lys Ile Thr Tyr Ile Asp Gly
50 55 60Asp Ala Gly Ile Leu Arg Tyr Arg Gly Tyr Asp Ile Ala Asp Leu Ala65 70 75 80Glu Asn Ala Thr Phe Asn Glu Val Ser Tyr Leu Leu Ile Asn Gly Glu
85 90 95Leu Pro Thr Pro Asp Glu Leu His Lys Phe Asn Asp Glu Ile Arg His
100 105 110His Thr Leu Leu Asp Glu Asp Phe Lys Ser Gln Phe Asn Val Phe Pro
115 120 125Arg Asp Ala His Pro Met Ala Thr Leu Ala Ser Ser Val Asn Ile Leu
130 135 140Ser Thr Tyr Tyr Gln Asp Gln Leu Asn Pro Leu Asp Glu Ala Gln Leu145 150 155 160Asp Lys Ala Thr Val Arg Leu Met Ala Lys Val Pro Met Leu Ala Ala
165 170 175Tyr Ala His Arg Ala Arg Lys Gly Ala Pro Tyr Met Tyr Pro Asp Asn
180 185 190Ser Leu Asn Ala Arg Glu Asn Phe Leu Arg Met Met Phe Gly Tyr Pro
195 200 205Thr Glu Pro Tyr Glu Ile Asp Pro Ile Met Val Lys Ala Leu Asp Lys
210 215 220Leu Leu Ile Leu His Ala Asp His Glu Gln Asn Cys Ser Thr Ser Thr225 230 235 240Val Arg Met Ile Gly Ser Ala Gln Ala Asn Met Phe Val Ser Ile Ala
245 250 255Gly Gly Ile Asn Ala Leu Ser Gly Pro Leu His Gly Gly Ala Asn Gln
260 265 270Ala Val Leu Glu Met Leu Glu Asp Ile Lys Ser Asn His Gly Gly Asp
275 280 285Ala Thr Glu Phe Met Asn Lys Val Lys Asn Lys Glu Asp Gly Val Arg
290 295 300Leu Met Gly Phe Gly His Arg Val Tyr Lys Asn Tyr Asp Pro Arg Ala305 310 315 320Ala Tle Val Lys Glu Thr Ala His Glu Ile Leu Glu His Leu Gly Gly
325 330 335Asp Asp Leu Leu Asp Leu Ala Ile Lys Leu Glu Glu Ile Ala Leu Ala
340 345 350Asp Asp Tyr Phe Ile Ser Arg Lys Leu Tyr Pro Asn Val Asp Phe Tyr
355 360 365Thr Gly Leu Ile Tyr Arg Ala Met Gly Phe Pro Thr Asp Phe Phe Thr
370 375 380Val Leu Phe Ala Ile Gly Arg Leu Pro Gly Trp Ile Ala His Tyr Arg385 390 395 400Glu Gln Leu Gly Ala Ala Gly Asn Lys Ile Asn Arg Pro Arg Gln Val
405 410 415Tyr Thr Gly Asn Glu Ser Arg Lys Leu Val Pro Arg Glu Glu Arg
420 425 430<210>547<211>942<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(919)<223>RXA02621<400>547atgtacctga ctggtgcgcg acacttcgct cactaaagtt tttaaagatt tcgcttgaag 60gcagaccata aggtctgcct tttcgcgtat taatgagtac atg tct gaa ctt att 115
Met Ser Glu Leu Ile
1 5tgt gga cca gct att ctc ttc gca cca gct gga cgt gct gag atc att 163Cys Gly Pro Ala Ile Leu Phe Ala Pro Ala Gly Arg Ala Glu Ile Ile
10 15 20cca aaa gca gca tcg aag gcc gat atg gtc atc att gat ttg gaa gat 211Pro Lys Ala Ala Ser Lys Ala Asp Met Val Ile Ile Asp Leu Glu Asp
25 30 35ggg gca ggg gag gta gac cgt gag gtc gcc tac agg aac att aga gaa 259Gly Ala Gly Glu Val Asp Arg Glu Val Ala Tyr Arg Asn Ile Arg Glu
40 45 50tcg ggg ttg gat cct aaa cga acc att gtg aga acc gta ggg ccg agc 307Ser Gly Leu Asp Pro Lys Arg Thr Ile Val Arg Thr Val Gly Pro Ser
55 60 65gat cca cac ttt ttg gct gac gtg gag atg gtg aag tcc acg gat ttc 355Asp Pro His Phe Leu Ala Asp Val Glu Met Val Lys Ser Thr Asp Phe70 75 80 85aca ctt gtt atg gtt cct aaa ctt ctt ggc agc gtg cct gag gaa tta 403Thr Leu Val Met Val Pro Lys Leu Leu Gly Ser Val Pro Glu Glu Leu
90 95 100gat ggc ctc aac att atc gcc atg att gaa acc cct cag gct gca acc 451Asp Gly Leu Asn Ile Ile Ala Met Ile Glu Thr Pro Gln Ala Ala Thr
105 110 115agc att cct cag att gct gcg gac cct aaa gtc gtt gga atg ttc tgg 499Ser Ile Pro Gln Ile Ala Ala Asp Pro Lys Val Val Gly Met Phe Trp
120 125 130ggc gcg gag gat ctc aca cac ctc ttg gga ggc act cat tct agg ttc 547Gly Ala Glu Asp Leu Thr His Leu Leu Gly Gly Thr His Ser Arg Phe
135 140 145ttg ggt gat gag tcc aat gaa ggc tcc tac cga gac acc atg agg ctt 595Leu Gly Asp Glu Ser Asn Glu Gly Ser Tyr Arg Asp Thr Met Arg Leu150 155 160 165aca cgc gcc ctg atg cac ctc cac gcg gcg gcg aat ggg aag ttc acc 643Thr Arg Ala Leu Met His Leu His Ala Ala Ala Asn Gly Lys Phe Thr
170 175 180att gat gcc atc cat gcg gat ttc cac gat gaa gag ggc ctc tat tta 691Ile Asp Ala Ile His Ala Asp Phe His Asp Glu Glu Gly Leu Tyr Leu
185 190 195gaa gcg gtc gat gct gcg cgg act ggt ttc gct ggc acc gca tgc att 739Glu Ala Val Asp Ala Ala Arg Thr Gly Phe Ala Gly Thr Ala Cys Ile
200 205 210cac ccc aag cag atc gag att gtt cgg aga gcc tat cgg cca gag gct 787His Pro Lys Gln Ile Glu Ile Val Arg Arg Ala Tyr Arg Pro Glu Ala
215 220 225aac cag ttg gag tgg gcg aag aaa gtg gtg gag gaa gca gaa aac cat 835Asn Gln Leu Glu Trp Ala Lys Lys Val Val Glu Glu Ala Glu Asn His230 235 240 245cca ggt gcg ttc aaa ctg gat ggt cag atg att gat gct ccg ttg att 883Pro Gly Ala Phe Lys Leu Asp Gly Gln Met Ile Asp Ala Pro Leu Ile
250 255 260tcg cag gcg cgg atg gtt att tcg cgt cag cct gct tgattagttc 929Ser Gln Ala Arg Met Val Ile Ser Arg Gln Pro Ala
265 270aagcgttttt tcg 942<210>548<211>273<212>PRT<213>谷氨酸棒杆菌<400>548Met Ser Glu Leu Ile Cys Gly Pro Ala Ile Leu Phe Ala Pro Ala Gly1 5 10 15Arg Ala Glu Ile Ile Pro Lys Ala Ala Ser Lys Ala Asp Met Val Ile
20 25 30Ile Asp Leu Glu Asp Gly Ala Gly Glu Val Asp Arg Glu Val Ala Tyr
35 40 45Arg Asn Ile Arg Glu Ser Gly Leu Asp Pro Lys Arg Thr Ile Val Arg
50 55 60Thr Val Gly Pro Ser Asp Pro His Phe Leu Ala Asp Val Glu Met Val65 70 75 80Lys Ser Thr Asp Phe Thr Leu Val Met Val Pro Lys Leu Leu Gly Ser
85 90 95Val Pro Glu Glu Leu Asp Gly Leu Asn Ile Ile Ala Met Ile Glu Thr
100 105 110Pro Gln Ala Ala Thr Ser Ile Pro Gln Ile Ala Ala Asp Pro Lys Val
115 120 125Val Gly Met Phe Trp Gly Ala Glu Asp Leu Thr His Leu Leu Gly Gly
130 135 140Thr His Ser Arg Phe Leu Gly Asp Glu Ser Asn Glu Gly Ser Tyr Arg145 150 155 160Asp Thr Met Arg Leu Thr Arg Ala Leu Met His Leu His Ala Ala Ala
165 170 175Asn Gly Lys Phe Thr Ile Asp Ala Ile His Ala Asp Phe His Asp Glu
180 185 190Glu Gly Leu Tyr Leu Glu Ala Val Asp Ala Ala Arg Thr Gly Phe Ala
195 200 205Gly Thr Ala Cys Ile His Pro Lys Gln Ile Glu Ile Val Arg Arg Ala
210 215 220Tyr Arg Pro Glu Ala Asn Gln Leu Glu Trp Ala Lys Lys Val Val Glu225 230 235 240Glu Ala Glu Asn His Pro Gly Ala Phe Lys Leu Asp Gly Gln Met Ile
245 250 255Asp Ala Pro Leu Ile Ser Gln Ala Arg Met Val Ile Ser Arg Gln Pro
260 265 270Ala<210>549<211>2337<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2314)<223>RXN00519<400>549tttccatgcg gggctgaaac tgccaccata ggcgccagca attagtagaa cactgtattc 60taggtagctg aacaaaagag cccatcaacc aaggagactc atg gct aag atc atc 115
Met Ala Lys Ile Ile
1 5tgg acc cgc acc gac gaa gca ccg ctg ctc gcg acc tac tcg ctg aag 163Trp Thr Arg Thr Asp Glu Ala Pro Leu Leu Ala Thr Tyr Ser Leu Lys
10 15 20ccg gtc gtc gag gca ttt gct gct acc gcg ggc att gag gtc gag acc 211Pro Val Val Glu Ala Phe Ala Ala Thr Ala Gly Ile Glu Val Glu Thr
25 30 35cgg gac att tca ctc gct gga cgc atc ctc gcc cag ttc cca gag cgc 259Arg Asp Ile Ser Leu Ala Gly Arg Ile Leu Ala Gln Phe Pro Glu Arg
40 45 50ctc acc gaa gat cag aag gta ggc aac gca ctc gca gaa ctc ggc gag 307Leu Thr Glu Asp Gln Lys Val Gly Asn Ala Leu Ala Glu Leu Gly Glu
55 60 65ctt gct aag act cct gaa gca aac atc att aag ctt cca aac atc tcc 355Leu Ala Lys Thr Pro Glu Ala Asn Ile Ile Lys Leu Pro Asn Ile Ser70 75 80 85gct tct gtt cca cag ctc aag gct gct att aag gaa ctg cag gac cag 403Ala Ser Val Pro Gln Leu Lys Ala Ala Ile Lys Glu Leu Gln Asp Gln
90 95 100ggc tac gac atc cca gaa ctg cct gat aac gcc acc acc gac gag gaa 451Gly Tyr Asp Ile Pro Glu Leu Pro Asp Asn Ala Thr Thr Asp Glu Glu
105 110 115aaa gac atc ctc gca cgc tac aac gct gtt aag ggt tcc gct gtg aac 499Lys Asp Ile Leu Ala Arg Tyr Asn Ala Val Lys Gly Ser Ala Val Asn
120 125 130cca gtg ctg cgt gaa ggc aac tct gac cgc cgc gca cca atc gct gtc 547Pro Val Leu Arg Glu Gly Asn Ser Asp Arg Arg Ala Pro Ile Ala Val
135 140 145aag aac ttt gtt aag aag ttc cca cac agc atg gac gag tgg tat gca 595Lys Asn Phe Val Lys Lys Phe Pro His Arg Met Gly Glu Trp Ser Ala150 155 160 165gat tcc aag acc aac gtt gca acc atg gat gca aac gac ttc cgc cac 643Asp Ser Lys Thr Asn Val Ala Thr Met Asp Ala Asn Asp Phe Arg His
170 175 180aac gag aag tcc atc atc ctc gac gct gct gat gaa gtt cag atc aag 691Asn Glu Lys Ser Ile Ile Leu Asp Ala Ala Asp Glu Val Gln Ile Lys
185 190 195cac atc gca gct gac ggc acc gag acc atc ctc aag gac agc ctc aag 739His Ile Ala Ala Asp Gly Thr Glu Thr Ile Leu Lys Asp Ser Leu Lys
200 205 210ctt ctt gaa ggc gaa gtt cta gac gga acc gtt ctg tcc gca aag gca 787Leu Leu Glu Gly Glu Val Leu Asp Gly Thr Val Leu Ser Ala Lys Ala
215 220 225ctg gac gca ttc ctt ctc gag cag gtc gct cgc gca aag gca gaa ggt 835Leu Asp Ala Phe Leu Leu Glu Gln Val Ala Arg Ala Lys Ala Glu Gly230 235 240 245atc ctc ttc tcc gca cac ctg aag gcc acc atg atg aag gtc tcc gac 883Ile Leu Phe Ser Ala His Leu Lys Ala Thr Met Met Lys Val Ser Asp
250 255 260cca atc atc ttc ggc cac gtt gtg cgc gct tac ttc gca gac gtt ttc 931Pro Ile Ile Phe Gly His Val Val Arg Ala Tyr Phe Ala Asp Val Phe
265 270 275gca cag tac ggt gag cag ctg ctc gca gct ggc ctc aac ggc gaa aac 979Ala Gln Tyr Gly Glu Gln Leu Leu Ala Ala Gly Leu Asn Gly Glu Asn
280 285 290ggc ctc gct gca atc ctc tcc ggc ttg gag tcc ctg gac aac ggc gaa 1027Gly Leu Ala Ala Ile Leu Ser Gly Leu Glu Ser Leu Asp Asn Gly Glu
295 300 305gaa atc aag gct gca ttc gag aag ggc ttg gaa gac ggc cca gac ctg 1075Glu Ile Lys Ala Ala Phe Glu Lys Gly Leu Glu Asp Gly Pro Asp Leu310 315 320 325gcc atg gtt aac tcc gct cgc ggc atc acc aac ctg cat gtc cct tcc 1123Ala Met Val Asn Ser Ala Arg Gly Ile Thr Asn Leu His Val Pro Ser
330 335 340gat gtc atc gtg gac gct tcc atg cca gca atg att cgt acc tcc ggc 1171Asp Val Ile Val Asp Ala Ser Met Pro Ala Met Ile Arg Thr Ser Gly
345 350 355cac atg tgg aac aaa gac gac cag gag cag gac acc ctg gca atc atc 1219His Met Trp Asn Lys Asp Asp Gln Glu Gln Asp Thr Leu Ala Ile Ile
360 365 370cca gac tcc tcc tac gct ggc gtc tac cag acc gtt atc gaa gac tgc 1267Pro Asp Ser Ser Tyr Ala Gly Val Tyr Gln Thr Val Ile Glu Asp Cys
375 380 385cgc aag aac ggc gca ttc gat cca acc acc atg ggt acc gtc cct aac 1315Arg Lys Asn Gly Ala Phe Asp Pro Thr Thr Met Gly Thr Val Pro Asn390 395 400 405gtt ggt ctg atg gct cag aag gct gaa gag tac ggc tcc cat gac aag 1363Val Gly Leu Met Ala Gln Lys Ala Glu Glu Tyr Gly Ser His Asp Lys
410 415 420acc ttc cgc atc gaa gca gac ggt gtg gtt cag gtt gtt tcc tcc aac 1411Thr Phe Arg Ile Glu Ala Asp Gly Val Val Gln Val Val Ser Ser Asn
425 430 435ggc gac gtt ctc atc gag cac gac gtt gag gca aat gac atc tgg cgt 1459Gly Asp Val Leu Ile Glu His Asp Val Glu Ala Asn Asp Ile Trp Arg
440 445 450gca tgc cag gtc aag gat gcc cca atc cag gat tgg gta aag ctt gct 1507Ala Cys Gln Val Lys Asp Ala Pro Ile Gln Asp Trp Val Lys Leu Ala
455 460 465gtc acc cgc tcc cgt ctc tcc gga atg cct gca gtg ttc tgg ttg gat 1555Val Thr Arg Ser Arg Leu Ser Gly Met Pro Ala Val Phe Trp Leu Asp470 475 480 485cca gag cgc gca cac gac cgc aac ctg gct tcc ctc gtt gag aag tac 1603Pro Glu Arg Ala His Asp Arg Asn Leu Ala Ser Leu Val Glu Lys Tyr
490 495 500ctg gct gac cac gac acc gag ggc ctg gac atc cag atc ctc tcc cct 1651Leu Ala Asp His Asp Thr Glu Gly Leu Asp Ile Gln Ile Leu Ser Pro
505 510 515gtt gag gca acc cag ctc tcc atc gac cgc atc cgc cgt ggc gag gac 1699Val Glu Ala Thr Gln Leu Ser Ile Asp Arg Ile Arg Arg Gly Glu Asp
520 525 530acc atc tct gtc acc ggt aac gtt ctg cgt gac tac aac acc gac ctc 1747Thr Ile Ser Val Thr Gly Asn Val Leu Arg Asp Tyr Asn Thr Asp Leu
535 540 545ttc cca atc ctg gag ctg ggc acc tct gca aag atg ctg tct gtc gtt 1795Phe Pro Ile Leu Glu Leu Gly Thr Ser Ala Lys Met Leu Ser Val Val550 555 560 565cct ttg atg gct ggc ggc gga ctg ttc gag acc ggt gct ggt gga tct 1843Pro Leu Met Ala Gly Gly Gly Leu Phe Glu Thr Gly Ala Gly Gly Ser
570 575 580gct cct aag cac gtc cag cag gtt cag gaa gaa aac cac ctg cgt tgg 1891Ala Pro Lys His Val Gln Gln Val Gln Glu Glu Asn His Leu Arg Trp
585 590 595gat tcc ctc ggt gag ttc ctc gca ctg gct gag tcc ttc cgc cac gag 1939Asp Ser Leu Gly Glu Phe Leu Ala Leu Ala Glu Ser Phe Arg His Glu
600 605 610ctc aac aac aac ggc aac acc aag gcc ggc gtt ctg gct gac gct ctg 1987Leu Asn Asn Asn Gly Asn Thr Lys Ala Gly Val Leu Ala Asp Ala Leu
615 620 625gac aag gca act gag aag ctg ctg aac gaa gag aag tcc cca tcc cgc 2035Asp Lys Ala Thr Glu Lys Leu Leu Asn Glu Glu Lys Ser Pro Ser Arg630 635 640 645aag gtt ggc gag atc gac aac cgt ggc tcc cac ttc tgg ctg acc aag 2083Lys Val Gly Glu Ile Asp Asn Arg Gly Ser His Phe Trp Leu Thr Lys
650 655 660ttc tgg gct gac gag ctc gct gct cag acc gag gac gca gat ctg gct 2131Phe Trp Ala Asp Glu Leu Ala Ala Gln Thr Glu Asp Ala Asp Leu Ala
665 670 675gct acc ttc gca cca gtc gca gaa gca ctg aac aca ggc gct gca gac 2179Ala Thr Phe Ala Pro Val Ala Glu Ala Leu Asn Thr Gly Ala Ala Asp
680 685 690atc gat gct gca ctg ctc gca gtt cag ggt gga gca act gac ctt ggt 2227Ile Asp Ala Ala Leu Leu Ala Val Gln Gly Gly Ala Thr Asp Leu Gly
695 700 705ggc tac tac tcc cct aac gag gag aag ctc acc aac atc atg cgc cca 2275Gly Tyr Tyr Ser Pro Asn Glu Glu Lys Leu Thr Asn Ile Met Arg Pro710 715 720 725gtc gca cag ttc aac gag atc gtt gac gca ctg aag aag taaagtctct 2324Val Ala Gln Phe Asn Glu Ile Val Asp Ala Leu Lys Lys
730 735tcacaaaaag cgc 2337<210>550<211>738<212>PRT<213>谷氨酸棒杆菌<400>550Met Ala Lys Ile Ile Trp Thr Arg Thr Asp Glu Ala Pro Leu Leu Ala1 5 10 15Thr Tyr Ser Leu Lys Pro Val Val Glu Ala Phe Ala Ala Thr Ala Gly
20 25 30Ile Glu Val Glu Thr Arg Asp Ile Ser Leu Ala Gly Arg Ile Leu Ala
35 40 45Gln Phe Pro Glu Arg Leu Thr Glu Asp Gln Lys Val Gly Asn Ala Leu
50 55 60Ala Glu Leu Gly Glu Leu Ala Lys Thr Pro Glu Ala Asn Ile Ile Lys65 70 75 80Leu Pro Asn Ile Ser Ala Ser Val Pro Gln Leu Lys Ala Ala Ile Lys
85 90 95Glu Leu Gln Asp Gln Gly Tyr Asp Ile Pro Glu Leu Pro Asp Asn Ala
100 105 110Thr Thr Asp Glu Glu Lys Asp Ile Leu Ala Arg Tyr Asn Ala Val Lys
115 120 125Gly Ser Ala Val Asn Pro Val Leu Arg Glu Gly Asn Ser Asp Arg Arg
130 135 140Ala Pro Ile Ala Val Lys Asn Phe Val Lys Lys Phe Pro His Arg Met145 150 155 160Gly Glu Trp Ser Ala Asp Ser Lys Thr Asn Val Ala Thr Met Asp Ala
165 170 175Asn Asp Phe Arg His Asn Glu Lys Ser Ile Ile Leu Asp Ala Ala Asp
180 185 190Glu Val Gln Ile Lys His Ile Ala Ala Asp Gly Thr Glu Thr Ile Leu
195 200 205Lys Asp Ser Leu Lys Leu Leu Glu Gly Glu Val Leu Asp Gly Thr Val
210 215 220Leu Ser Ala Lys Ala Leu Asp Ala Phe Leu Leu Glu Gln Val Ala Arg225 230 235 240Ala Lys Ala Glu Gly Ile Leu Phe Ser Ala His Leu Lys Ala Thr Met
245 250 255Met Lys Val Ser Asp Pro Ile Ile Phe Gly His Val Val Arg Ala Tyr
260 265 270Phe Ala Asp Val Phe Ala Gln Tyr Gly Glu Gln Leu Leu Ala Ala Gly
275 280 285Leu Asn Gly Glu Asn Gly Leu Ala Ala Ile Leu Ser Gly Leu Glu Ser
290 295 300Leu Asp Asn Gly Glu Glu Ile Lys Ala Ala Phe Glu Lys Gly Leu Glu305 310 315 320Asp Gly Pro Asp Leu Ala Met Val Asn Ser Ala Arg Gly Ile Thr Asn
325 330 335Leu His Val Pro Ser Asp Val Ile Val Asp Ala Ser Met Pro Ala Met
340 345 350Ile Arg Thr Ser Gly His Met Trp Asn Lys Asp Asp Gln Glu Gln Asp
355 360 365Thr Leu Ala Ile Ile Pro Asp Ser Ser Tyr Ala Gly Val Tyr Gln Thr
370 375 380Val Ile Glu Asp Cys Arg Lys Asn Gly Ala Phe Asp Pro Thr Thr Met385 390 395 400Gly Thr Val Pro Asn Val Gly Leu Met Ala Gln Lys Ala Glu Glu Tyr
405 410 415Gly Ser His Asp Lys Thr Phe Arg Ile Glu Ala Asp Gly Val Val Gln
420 425 430Val Val Ser Ser Asn Gly Asp Val Leu Ile Glu His Asp Val Glu Ala
435 440 445Asn Asp Ile Trp Arg Ala Cys Gln Val Lys Asp Ala Pro Ile Gln Asp
450 455 460Trp Val Lys Leu Ala Val Thr Arg Ser Arg Leu Ser Gly Met Pro Ala465 470 475 480Val Phe Trp Leu Asp Pro Glu Arg Ala His Asp Arg Asn Leu Ala Ser
485 490 495Leu Val Glu Lys Tyr Leu Ala Asp His Asp Thr Glu Gly Leu Asp Ile
500 505 510Gln Ile Leu Ser Pro Val Glu Ala Thr Gln Leu Ser Ile Asp Arg Ile
515 520 525Arg Arg Gly Glu Asp Thr Ile Ser Val Thr Gly Asn Val Leu Arg Asp
530 535 540Tyr Asn Thr Asp Leu Phe Pro Ile Leu Glu Leu Gly Thr Ser Ala Lys545 550 555 560Met Leu Ser Val Val Pro Leu Met Ala Gly Gly Gly Leu Phe Glu Thr
565 570 575Gly Ala Gly Gly Ser Ala Pro Lys His Val Gln Gln Val Gln Glu Glu
580 585 590Asn His Leu Arg Trp Asp Ser Leu Gly Glu Phe Leu Ala Leu Ala Glu
595 600 605Ser Phe Arg His Glu Leu Asn Asn Asn Gly Asn Thr Lys Ala Gly Val
610 615 620Leu Ala Asp Ala Leu Asp Lys Ala Thr Glu Lys Leu Leu Asn Glu Glu625 630 635 640Lys Ser Pro Ser Arg Lys Val Gly Glu Ile Asp Asn Arg Gly Ser His
645 650 655Phe Trp Leu Thr Lys Phe Trp Ala Asp Glu Leu Ala Ala Gln Thr Glu
660 665 670Asp Ala Asp Leu Ala Ala Thr Phe Ala Pro Val Ala Glu Ala Leu Asn
675 680 685Thr Gly Ala Ala Asp Ile Asp Ala Ala Leu Leu Ala Val Gln Gly Gly
690 695 700Ala Thr Asp Leu Gly Gly Tyr Tyr Ser Pro Asn Glu Glu Lys Leu Thr705 710 715 720Asn Ile Met Arg Pro Val Ala Gln Phe Asn Glu Ile Val Asp Ala Leu
725 730 735Lys Lys<210>551<211>1059<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1059)<223>FRXA00521<400>551ctc gac gct gct gat gaa gtt cag atc aag cac atc gca gct gac ggc 48Leu Asp Ala Ala Asp Glu Val Gln Ile Lys His Ile Ala Ala Asp Gly1 5 10 15acc gag acc atc ctc aag gac agc ctc aag ctt ctt gaa ggc gaa gtt 96Thr Glu Thr Ile Leu Lys Asp Ser Leu Lys Leu Leu Glu Gly Glu Val
20 25 30cta gac gga acc gtt ctg tcc gca aag gca ctg gac gca ttc ctt ctc 144Leu Asp Gly Thr Val Leu Ser Ala Lys Ala Leu Asp Ala Phe Leu Leu
35 40 45gag cag gtc gct cgc gca aag gca gaa ggt atc ctc ttc tcc gca cac 192Glu Gln Val Ala Arg Ala Lys Ala Glu Gly Ile Leu Phe Ser Ala His
50 55 60ctg aag gcc acc atg atg aag gtc tcc gac cca atc atc ttc ggc cac 240Leu Lys Ala Thr Met Met Lys Val Ser Asp Pro Ile Ile Phe Gly His65 70 75 80gtt gtg cgc gct tac ttc gca gac gtt ttc gca cag tac ggt gag cag 288Val Val Arg Ala Tyr Phe Ala Asp Val Phe Ala Gln Tyr Gly Glu Gln
85 90 95ctg ctc gca gct ggc ctc aaa ggc gaa aac ggc ctc gct gca atc ctc 336Leu Leu Ala Ala Gly Leu Asn Gly Glu Asn Gly Leu Ala Ala Ile Leu
100 105 110tcc ggc ttg gag tcc ctg gac aac ggc gaa gaa atc aag gct gca ttc 384Ser Gly Leu Glu Ser Leu Asp Asn Gly Glu Glu Ile Lys Ala Ala Phe
115 120 125gag aag ggc ttg gaa gac ggc cca gac ctg gcc atg gtt aac tcc gct 432Glu Lys Gly Leu Glu Asp Gly Pro Asp Leu Ala Met Val Asn Ser Ala
130 135 140cgc ggc atc acc aac ctg cat gtc cct tcc gat gtc atc gtg gac gct 480Arg Gly Ile Thr Asn Leu His Val Pro Ser Asp Val Ile Val Asp Ala145 150 155 160tcc atg cca gca atg att cgt acc tcc ggc cac atg tgg aac aaa gac 528Ser Met Pro Ala Met Ile Arg Thr Ser Gly His Met Trp Asn Lys Asp
165 170 175gac cag gag cag gac acc ctg gca atc atc cca gac tcc tcc tac gct 576Asp Gln Glu Gln Asp Thr Leu Ala Ile Ile Pro Asp Ser Ser Tyr Ala
180 185 190ggc gtc tac cag acc gtt atc gaa gac tgc cgc aag aac ggc gca ttc 624Gly Val Tyr Gln Thr Val Ile Glu Asp Cys Arg Lys Asn Gly Ala Phe
195 200 205gat cca acc acc atg ggt acc gtc cct aac gtt ggt ctg atg gct cag 672Asp Pro Thr Thr Met Gly Thr Val Pro Asn Val Gly Leu Met Ala Gln
210 215 220aag gct gaa gag tac ggc tcc cat gac aag acc ttc cgc atc gaa gca 720Lys Ala Glu Glu Tyr Gly Ser His Asp Lys Thr Phe Arg Ile Glu Ala225 230 235 240gac gtt gtg gtt cag gtt gtt tcc tcc aac ggc gac gtt ctc atc gag 768Asp Gly Val Val Gln Val Val Ser Ser Asn Gly Asp Val Leu Ile Glu
245 250 255cac gac gtt gag gca aat gac atc tgg cgt gca tgc cag gtc aag gat 816His Asp Val Glu Ala Asn Asp Ile Trp Arg Ala Cys Gln Val Lys Asp
260 265 270gcc cca atc cag gat tgg gta aag ctt gct gtc acc cgc tcc cgt ctc 864Ala Pro Ile Gln Asp Trp Val Lys Leu Ala Val Thr Arg Ser Arg Leu
275 280 285tcc gga atg cct gca gtg ttc tgg ttg gat cca gag cgc gca cac gac 912Ser Gly Met Pro Ala Val Phe Trp Leu Asp Pro Glu Arg Ala His Asp
290 295 300cgc aac ctg gct tcc ctc gtt gag aag tac ctg gct gac cac gac acc 960Arg Asn Leu Ala Ser Leu Val Glu Lys Tyr Leu Ala Asp His Asp Thr305 310 315 320gag ggc ctg gac atc cag atc ctc tac cct gtt gag gca acc cag ctc 1008Glu Gly Leu Asp Ile Gln Ile Leu Tyr Pro Val Glu Ala Thr Gln Leu
325 330 335tcc atc gac cgc atc cgc cgt ggc gag gac acc atc tct gtc acc ggt 1056Ser Ile Asp Arg Ile Arg Arg Gly Glu Asp Thr Ile Ser Val Thr Gly
340 345 350aac 1059Asn<210>552<211>353<212>PRT<213>谷氨酸棒杆菌<400>552Leu Asp Ala Ala Asp Glu Val Gln Ile Lys His Ile Ala Ala Asp Gly1 5 10 15Thr Glu Thr Ile Leu Lys Asp Ser Leu Lys Leu Leu Glu Gly Glu Val
20 25 30Leu Asp Gly Thr Val Leu Ser Ala Lys Ala Leu Asp Ala Phe Leu Leu
35 40 45Glu Gln Val Ala Arg Ala Lys Ala Glu Gly Ile Leu Phe Ser Ala His
50 55 60Leu Lys Ala Thr Met Met Lys Val Ser Asp Pro Ile Ile Phe Gly His65 70 75 80Val Val Arg Ala Tyr Phe Ala Asp Val Phe Ala Gln Tyr Gly Glu Gln
85 90 95Leu Leu Ala Ala Gly Leu Asn Gly Glu Asn Gly Leu Ala Ala Ile Leu
100 105 110Ser Gly Leu Glu Ser Leu Asp Asn Gly Glu Glu Ile Lys Ala Ala Phe
115 120 125Glu Lys Gly Leu Glu Asp Gly Pro Asp Leu Ala Met Val Asn Ser Ala
130 135 140Arg Gly Ile Thr Asn Leu His Val Pro Ser Asp Val Ile Val Asp Ala145 150 155 160Ser Met Pro Ala Met Ile Arg Thr Ser Gly His Met Trp Asn Lys Asp
165 170 175Asp Gln Glu Gln Asp Thr Leu Ala Ile Ile Pro Asp Ser Ser Tyr Ala
180 185 190Gly Val Tyr Gln Thr Val Ile Glu Asp Cys Arg Lys Asn Gly Ala Phe
195 200 205Asp Pro Thr Thr Met Gly Thr Val Pro Asn Val Gly Leu Met Ala Gln
210 215 220Lys Ala Glu Glu Tyr Gly Ser His Asp Lys Thr Phe Arg Ile Glu Ala225 230 235 240Asp Gly Val Val Gln Val Val Ser Ser Asn Gly Asp Val Leu Ile Glu
245 250 255His Asp Val Glu Ala Asn Asp Ile Trp Arg Ala Cys Gln Val Lys Asp
260 265 270Ala Pro Ile Gln Asp Trp Val Lys Leu Ala Val Thr Arg Ser Arg Leu
275 280 285Ser Gly Met Pro Ala Val Phe Trp Leu Asp Pro Glu Arg Ala His Asp
290 295 300Arg Asn Leu Ala Ser Leu Val Glu Lys Tyr Leu Ala Asp His Asp Thr305 310 315 320Glu Gly Leu Asp Ile Gln Ile Leu Tyr Pro Val Glu Ala Thr Gln Leu
325 330 335Ser Ile Asp Arg Ile Arg Arg Gly Glu Asp Thr Ile Ser Val Thr Gly
340 345 350Asn<210>553<211>1694<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1671)<223>RXN02209<400>553acc gag tcc acc gtt gtt cct tcc atc gct ggc cct aag cgc cca cag 48Thr Glu Ser Thr Val Val Pro Ser Ile Ala Gly Pro Lys Arg Pro Gln1 5 10 15gac cgc atc ctt ctc tcc gag gca aag gag cag ttc cgt aag gat ctg 96Asp Arg Ile Leu Leu Ser Glu Ala Lys Glu Gln Phe Arg Lys Asp Leu
20 25 30cca acc tac acc gac gac gct gtt tcc gta gac acc tcc atc cct gca 144Pro Thr Tyr Thr Asp Asp Ala Val Ser Val Asp Thr Ser Ile Pro Ala
35 40 45acc cgc atg gtt aac gaa ggt ggc gga cag cct gaa ggc ggc gtc gaa 192Thr Arg Met Val Asn Glu Gly Gly Gly Gln Pro Glu Gly Gly Val Glu
50 55 60gct gac aac tac aac gct tcc tgg gct ggc tcc ggc gag tcc ttg gct 240Ala Asp Asn Tyr Asn Ala Ser Trp Ala Gly Ser Gly Glu Ser Leu Ala65 70 75 80act ggc gca gaa gga cgt cct tcc aag cca gtc acc gtt gca tcc cca 288Thr Gly Ala Glu Gly Arg Pro Ser Lys Pro Val Thr Val Ala Ser Pro
85 90 95cag ggt ggc gag tac acc atc gac cac ggc atg gtt gca att gca tcc 336Gln Gly Gly Glu Tyr Thr Ile Asp His Gly Met Val Ala Ile Ala Ser
100 105 110atc acc tct tgc acc aac acc tct aac cca tcc gtg atg atc ggc gct 384Ile Thr Ser Cys Thr Asn Thr Ser Asn Pro Ser Val Met Ile Gly Ala
115 120 125ggc ctg atc gca cgt aag gca gca gaa aag ggc ctc aag tcc aag cct 432Gly Leu Ile Ala Arg Lys Ala Ala Glu Lys Gly Leu Lys Ser Lys Pro
130 135 140tgg gtt aag acc atc tgt gca cca ggt tcc cag gtt gtc gac ggc tac 480Trp Val Lys Thr Ile Cys Ala Pro Gly Ser Gln Val Val Asp Gly Tyr145 150 155 160tac cag cgc gca gac ctc tgg aag gac ctt gag gcc atg ggc ttc tac 528Tyr Gln Arg Ala Asp Leu Trp Lys Asp Leu Glu Ala Met Gly Phe Tyr
165 170 175ctc tcc ggc ttc ggc tgc acc acc tgt att ggt aac tcc ggc cca ctg 576Leu Ser Gly Phe Gly Cys Thr Thr Cys Ile Gly Asn Ser Gly Pro Leu
180 185 190cca gag gaa atc tcc gct gcg atc aac gag cac gac ctg acc gca acc 624Pro Glu Glu Ile Ser Ala Ala Ile Asn Glu His Asp Leu Thr Ala Thr
195 200 205gca gtt ttg tcc ggt aac cgt aac ttc gag gga cgt atc tcc cct gac 672Ala Val Leu Ser Gly Asn Arg Asn Phe Glu Gly Arg Ile Ser Pro Asp
210 215 220gtt aag atg aac tac ctg gca tcc cca atc atg gtc att gct tac gca 720Val Lys Met Asn Tyr Leu Ala Ser Pro Ile Met Val Ile Ala Tyr Ala225 230 235 240atc gct ggc acc atg gac ttc gac ttc gag aac gaa gct ctt gga cag 768Ile Ala Gly Thr Met Asp Phe Asp Phe Glu Asn Glu Ala Leu Gly Gln
245 250 255gac cag gac ggc aac gac gtc ttc ctg aag gac atc tgg cct tcc acc 816Asp Gln Asp Gly Asn Asp Val Phe Leu Lys Asp Ile Trp Pro Ser Thr
260 265 270gag gaa atc gaa gac acc atc cag cag gca atc tcc cgt gag ctt tac 864Glu Glu Ile Glu Asp Thr Ile Gln Gln Ala Ile Ser Arg Glu Leu Tyr
275 280 285gaa gct gac tac gca gat gtc ttc aag ggt gac aag cag tgg cag gaa 912Glu Ala Asp Tyr Ala Asp Val Phe Lys Gly Asp Lys Gln Trp Gln Glu
290 295 300ctc gat gtt cct acc ggt gac acc ttc gag tgg gac gag aac tcc acc 960Leu Asp Val Pro Thr Gly Asp Thr Phe Glu Trp Asp Glu Asn Ser Thr305 310 315 320tac atc cgc aag gca cct tac ttc gac ggc atg cct gtc gag cca gtg 1008Tyr Ile Arg Lys Ala Pro Tyr Phe Asp Gly Met Pro Val Glu Pro Val
325 330 335gca gtc acc gac atc cag ggc gca cgc gtt ctg gct aag ctc ggc gac 1056Ala Val Thr Asp Ile Gln Gly Ala Arg Val Leu Ala Lys Leu Gly Asp
340 345 350tct gtc acc acc gac cac atc tcc cct gct tcc tcc att aag cca ggt 1104Ser Val Thr Thr Asp His Ile Ser Pro Ala Ser Ser Ile Lys Pro Gly
355 360 365acc cct gca gct cag tac ttg gat gag cac ggt gtg gaa cgc cac gac 1152Thr Pro Ala Ala Gln Tyr Leu Asp Glu His Gly Val Glu Arg His Asp
370 375 380tac aac tcc ctg ggt tcc agg cgt ggt aac cac gag gtc atg atg cgc 1200Tyr Asn Ser Leu Gly Ser Arg Arg Gly Asn His Glu Val Met Met Arg385 390 395 400ggc acc ttc gcc aac atc cgc ctc cag aac cag ctg gtt gac atc gca 1248Gly Thr Phe Ala Asn Ile Arg Leu Gln Asn Gln Leu Val Asp Ile Ala
405 410 415ggt ggc tac acc cgc gac ttc acc cag gag ggt gct cca cag gcg ttc 1296Gly Gly Tyr Thr Arg Asp Phe Thr Gln Glu Gly Ala Pro Gln Ala Phe
420 425 430atc tac gac gct tcc gtc aac tac aag gct gct ggc att ccg ctg gtc 1344Ile Tyr Asp Ala Ser Val Asn Tyr Lys Ala Ala Gly Ile Pro Leu Val
435 440 445gtc ttg ggc ggc aag gag tac ggc acc ggt tct tcc cgt gac tgg gca 1392Val Leu Gly Gly Lys Glu Tyr Gly Thr Gly Ser Ser Arg Asp Trp Ala
450 455 460gct aag ggc act aac ctg ctc gga att cgc gca gtt atc acc gag tcc 1440Ala Lys Gly Thr Asn Leu Leu Gly Ile Arg Ala Val Ile Thr Glu Ser465 470 475 480ttc gag cgt att cac cgc tcc aac ctc atc ggt atg ggc gtt gtc cca 1488Phe Glu Arg Ile His Arg Ser Asn Leu Ile Gly Met Gly Val Val Pro
485 490 495ctg cag ttc cct gca ggc gaa tcc cac gag tcc ctg ggc ctt gac ggc 1536Leu Gln Phe Pro Ala Gly Glu Ser His Glu Ser Leu Gly Leu Asp Gly
500 505 510acc gag acc ttc gac atc acc gga ctg acc gca ctt aac gag ggc gag 1584Thr Glu Thr Phe Asp Ile Thr Gly Leu Thr Ala Leu Asn Glu Gly Glu
515 520 525act cct aag act gtc aag gtc acc gca acc aag gag aac ggc gac gtc 1632Thr Pro Lys Thr Val Lys Val Thr Ala Thr Lys Glu Asn Gly Asp Val
530 535 540gtc gag ttc gac gca att tgt ccg cat cga cac ccc agg tgaggctgac 1681Val Glu Phe Asp Ala Ile Cys Pro His Arg His Pro Arg545 550 555tactaccgcc acg 1694<210>554<211>557<212>PRT<213>谷氨酸棒杆菌<400>554Thr Glu Ser Thr Val Val Pro Ser Ile Ala Gly Pro Lys Arg Pro Gln1 5 10 15Asp Arg Ile Leu Leu Ser Glu Ala Lys Glu Gln Phe Arg Lys Asp Leu
20 25 30Pro Thr Tyr Thr Asp Asp Ala Val Ser Val Asp Thr Ser Ile Pro Ala
35 40 45Thr Arg Met Val Asn Glu Gly Gly Gly Gln Pro Glu Gly Gly Val Glu
50 55 60Ala Asp Asn Tyr Asn Ala Ser Trp Ala Gly Ser Gly Glu Ser Leu Ala65 70 75 80Thr Gly Ala Glu Gly Arg Pro Ser Lys Pro ValThr Val Ala Ser Pro
85 90 95Gln Gly Gly Glu Tyr Thr Ile Asp His Gly Met Val Ala Ile Ala Ser
100 105 110Ile Thr Ser Cys Thr Asn Thr Ser Asn Pro Ser Val Met Ile Gly Ala
115 120 125Gly Leu Ile Ala Arg Lys Ala Ala Glu Lys Gly Leu Lys Ser Lys Pro
130 135 140Trp Val Lys Thr Ile Cys Ala Pro Gly Ser Gln Val Val Asp Gly Tyr145 150 155 160Tyr Gln Arg Ala Asp Leu Trp Lys Asp Leu Glu Ala Met Gly Phe Tyr
165 170 175Leu Ser Gly Phe Gly Cys Thr Thr Cys Ile Gly Asn Ser Gly Pro Leu
180 185 190Pro Glu Glu Ile Ser Ala Ala Ile Asn Glu His Asp Leu Thr Ala Thr
195 200 205Ala Val Leu Ser Gly Asn Arg Asn Phe Glu Gly Arg Ile Ser Pro Asp
210 215 220Val Lys Met Asn Tyr Leu Ala Ser Pro Ile Met Val Ile Ala Tyr Ala225 230 235 240Ile Ala Gly Thr Met Asp Phe Asp Phe Glu Asn Glu Ala Leu Gly Gln
245 250 255Asp Gln Asp Gly Asn Asp Val Phe Leu Lys Asp Ile Trp Pro Ser Thr
260 265 270Glu Glu Ile Glu Asp Thr Ile Gln Gln Ala Ile Ser Arg Glu Leu Tyr
275 280 285Glu Ala Asp Tyr Ala Asp Val Phe Lys Gly Asp Lys Gln Trp Gln Glu
290 295 300Leu Asp Val Pro Thr Gly Asp Thr Phe Glu Trp Asp Glu Asn Ser Thr305 310 315 320Tyr Ile Arg Lys Ala Pro Tyr Phe Asp Gly Met Pro Val Glu Pro Val
325 330 335Ala Val Thr Asp Ile Gln Gly Ala Arg Val Leu Ala Lys Leu Gly Asp
340 345 350Ser Val Thr Thr Asp His Ile Ser Pro Ala Ser Ser Ile Lys Pro Gly
355 360 365Thr Pro Ala Ala Gln Tyr Leu Asp Glu His Gly Val Glu Arg His Asp
370 375 380Tyr Asn Ser Leu Gly Ser Arg Arg Gly Asn His Glu Val Met Met Arg385 390 395 400Gly Thr Phe Ala Asn Ile Arg Leu Gln Asn Gln Leu Val Asp Ile Ala
405 410 415Gly Gly Tyr Thr Arg Asp Phe Thr Gln Glu Gly Ala Pro Gln Ala Phe
420 425 430Ile Tyr Asp Ala Ser Val Asn Tyr Lys Ala Ala Gly Ile Pro Leu Val
435 440 445Val Leu Gly Gly Lys Glu Tyr Gly Thr Gly Ser Ser Arg Asp Trp Ala
450 455 460Ala Lys Gly Thr Asn Leu Leu Gly Ile Arg Ala Val Ile Thr Glu Ser465 470 475 480Phe Glu Arg Ile His Arg Ser Asn Leu Ile Gly Met Gly Val Val Pro
485 490 495Leu Gln Phe Pro Ala Gly Glu Ser His Glu Ser Leu Gly Leu Asp Gly
500 505 510Thr Glu Thr Phe Asp Ile Thr Gly Leu Thr Ala Leu Asn Glu Gly Glu
515 520 525Thr Pro Lys Thr Val Lys Val Thr Ala Thr Lys Glu Asn Gly Asp Val
530 535 540Val Glu Phe Asp Ala Ile Cys Pro His Arg His Pro Arg545 550 555<210>555<211>1682<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1659)<223>FRXA02209<400>555gtt gtt cct tcc atc gct ggc cct aag cgc cca cag gac cgc atc ctt 48Val Val Pro Ser Ile Ala Gly Pro Lys Arg Pro Gln Asp Arg Ile Leu1 5 10 15ctc tcc gag gca aag gag cag ttc cgt aag gat ctg cca acc tac acc 96Leu Ser Glu Ala Lys Glu Gln Phe Arg Lys Asp Leu Pro Thr Tyr Thr
20 25 30gac gac gct gtt tcc gta gac acc tcc atc cct gca acc cgc atg gtt 144Asp Asp Ala Val Ser Val Asp Thr Ser Ile Pro Ala Thr Arg Met Val
35 40 45aac gaa ggt ggc gga cag cct gaa ggc ggc gtc gaa gct gac aac tac 192Asn Glu Gly Gly Gly Gln Pro Glu Gly Gly Val Glu Ala Asp Asn Tyr
50 55 60aac gct tcc tgg gct ggc tcc ggc gag tcc ttg gct act ggc gca gaa 240Asn Ala Ser Trp Ala Gly Ser Gly Glu Ser Leu Ala Thr Gly Ala Glu65 70 75 80gga cgt cct tcc aag cca gtc acc gtt gca tcc cca cag ggt ggc gag 288Gly Arg Pro Ser Lys Pro Val Thr Val Ala Ser Pro Gln Gly Gly Glu
85 90 95tac acc atc gac cac ggc atg gtt gca att gca tcc atc acc tct tgc 336Tyr Thr Ile Asp His Gly Met Val Ala Ile Ala Ser Ile Thr Ser Cys
100 105 110acc aac acc tct aac cca tcc gtg atg atc ggc gct ggc ctg atc gca 384Thr Asn Thr Ser Asn Pro Ser Val Met Ile Gly Ala Gly Leu Ile Ala
115 120 125cgt aag gca gca gaa aag ggc ctc aag tcc aag cct tgg gtt aag acc 432Arg Lys Ala Ala Glu Lys Gly Leu Lys Ser Lys Pro Trp Val Lys Thr
130 135 140atc tgt gca cca ggt tcc cag gtt gtc gac ggc tac tac cag cgc gca 480Ile Cys Ala Pro Gly Ser Gln Val Val Asp Gly Tyr Tyr Gln Arg Ala145 150 155 160gac ctc tgg aag gac ctt gag gcc atg ggc ttc tac ctc tcc ggc ttc 528Asp Leu Trp Lys Asp Leu Glu Ala Met Gly Phe Tyr Leu Ser Gly Phe
165 170 175ggc tgc acc acc tgt att ggt aac tcc ggc cca ctg cca gag gaa atc 576Gly Cys Thr Thr Cys Ile Gly Asn Ser Gly Pro Leu Pro Glu Glu Ile
180 185 190tcc gct gcg atc aac gag cac gac ctg acc gca acc gca gtt ttg tcc 624Ser Ala Ala Ile Asn Glu His Asp Leu Thr Ala Thr Ala Val Leu Ser
195 200 205ggt aac cgt aac ttc gag gga cgt atc tcc cct gac gtt aag atg aac 672Gly Asn Arg Asn Phe Glu Gly Arg Ile Ser Pro Asp Val Lys Met Asn
210 215 220tac ctg gca tcc cca atc atg gtc att gct tac gca atc gct ggc acc 720Tyr Leu Ala Ser Pro Ile Met Val Ile Ala Tyr Ala Ile Ala Gly Thr225 230 235 240atg gac ttc gac ttc gag aac gaa gct ctt gga cag gac cag gac ggc 768Met Asp Phe Asp Phe Glu Asn Glu Ala Leu Gly Gln Asp Gln Asp Gly
245 250 255aac gac gtc ttc ctg aag gac atc tgg cct tcc acc gag gaa atc gaa 816Asn Asp Val Phe Leu Lys Asp Ile Trp Pro Ser Thr Glu Glu Ile Glu
260 265 270gac acc atc cag cag gca atc tcc cgt gag ctt tac gaa gct gac tac 864Asp Thr Ile Gln Gln Ala Ile Ser Arg Glu Leu Tyr Glu Ala Asp Tyr
275 280 285gca gat gtc ttc aag ggt gac aag cag tgg cag gaa ctc gat gtt cct 912Ala Asp Val Phe Lys Gly Asp Lys Gln Trp Gln Glu Leu Asp Val Pro
290 295 300acc ggt gac acc ttc gag tgg gac gag aac tcc acc tac atc cgc aag 960Thr Gly Asp Thr Phe Glu Trp Asp Glu Asn Ser Thr Tyr Ile Arg Lys305 310 315 320gca cct tac ttc gac ggc atg cct gtc gag cca gtg gca gtc acc gac 1008Ala Pro Tyr Phe Asp Gly Met Pro Val Glu Pro Val Ala Val Thr Asp
325 330 335atc cag ggc gca cgc gtt ctg gct aag ctc ggc gac tct gtc acc acc 1056Ile Gln Gly Ala Arg Val Leu Ala Lys Leu Gly Asp Ser Val Thr Thr
340 345 350gac cac atc tcc cct gct tcc tcc att aag cca ggt acc cct gca gct 1104Asp His Ile Ser Pro Ala Ser Ser Ile Lys Pro Gly Thr Pro Ala Ala
355 360 365cag tac ttg gat gag cac ggt gtg gaa cgc cac gac tac aac tcc ctg 1152Gln Tyr Leu Asp Glu His Gly Val Glu Arg His Asp Tyr Asn Ser Leu
370 375 380ggt tcc agg cgt ggt aac cac gag gtc atg atg cgc ggc acc ttc gcc 1200Gly Ser Arg Arg Gly Asn His Glu Val Met Met Arg Gly Thr Phe Ala385 390 395 400aac atc cgc ctc cag aac cag ctg gtt gac atc gca ggt ggc tac acc 1248Asn Ile Arg Leu Gln Asn Gln Leu Val Asp Ile Ala Gly Gly Tyr Thr
405 410 415cgc gac ttc acc cag gag ggt gct cca cag gcg ttc atc tac gac gct 1296Arg Asp Phe Thr Gln Glu Gly Ala Pro Gln Ala Phe Ile Tyr Asp Ala
420 425 430tcc gtc aac tac aag gct gct ggc att ccg ctg gtc gtc ttg ggc ggc 1344Ser Val Asn Tyr Lys Ala Ala Gly Ile Pro Leu Val Val Leu Gly Gly
435 440 445aag gag tac ggc acc ggt tct tcc cgt gac tgg gca gct aag ggc act 1392Lys Glu Tyr Gly Thr Gly Ser Ser Arg Asp Trp Ala Ala Lys Gly Thr
450 455 460aac ctg ctc gga att cgc gca gtt atc acc gag tcc ttc gag cgt att 1440Asn Leu Leu Gly Ile Arg Ala Val Ile Thr Glu Ser Phe Glu Arg Ile465 470 475 480cac cgc tcc aac ctc atc ggt atg ggc gtt gtc cca ctg cag ttc cct 1488His Arg Ser Asn Leu Ile Gly Met Gly Val Val Pro Leu Gln Phe Pro
485 490 495gca ggc gaa tcc cac gag tcc ctg ggc ctt gac ggc acc gag acc ttc 1536Ala Gly Glu Ser His Glu Ser Leu Gly Leu Asp Gly Thr Glu Thr Phe
500 505 510gac atc acc gga ctg acc gca ctt aac gag ggc gag act cct aag act 1584Asp Ile Thr Gly Leu Thr Ala Leu Asn Glu Gly Glu Thr Pro Lys Thr
515 520 525gtc aag gtc acc gca acc aag gag aac ggc gac gtc gtc gag ttc gac 1632Val Lys Val Thr Ala Thr Lys Glu Asn Gly Asp Val Val Glu Phe Asp
530 535 540gca att tgt ccg cat cga cac ccc agg tgaggctgac tactaccgcc 1679Ala Ile Cys Pro His Arg His Pro Arg545 550acg 1682<210>556<211>553<212>PRT<213>谷氨酸棒杆菌<400>556Val Val Pro Ser Ile Ala Gly Pro Lys Arg Pro Gln Asp Arg Ile Leu1 5 10 15Leu Ser Glu Ala Lys Glu Gln Phe Arg Lys Asp Leu Pro Thr Tyr Thr
20 25 30Asp Asp Ala Val Ser Val Asp Thr Ser Ile Pro Ala Thr Arg Met Val
35 40 45Asn Glu Gly Gly Gly Gln Pro Glu Gly Gly Val Glu Ala Asp Asn Tyr
50 55 60Asn Ala Ser Trp Ala Gly Ser Gly Glu Ser Leu Ala Thr Gly Ala Glu65 70 75 80Gly Arg Pro Ser Lys Pro Val Thr Val Ala Ser Pro Gln Gly Gly Glu
85 90 95Tyr Thr Ile Asp His Gly Met Val Ala Ile Ala Ser Ile Thr Ser Cys
100 105 110Thr Asn Thr Ser Asn Pro Ser Val Met Ile Gly Ala Gly Leu Ile Ala
115 120 125Arg Lys Ala Ala Glu Lys Gly Leu Lys Ser Lys Pro Trp Val Lys Thr
130 135 140Ile Cys Ala Pro Gly Ser Gln Val Val Asp Gly Tyr Tyr Gln Arg Ala145 150 155 160Asp Leu Trp Lys Asp Leu Glu Ala Met Gly Phe Tyr Leu Ser Gly Phe
165 170 175Gly Cys Thr Thr Cys Ile Gly Asn Ser Gly Pro Leu Pro Glu Glu Ile
180 185 190Ser Ala Ala Ile Asn Glu His Asp Leu Thr Ala Thr Ala Val Leu Ser
195 200 205Gly Asn Arg Asn Phe Glu Gly Arg Ile Ser Pro Asp Val Lys Met Asn
210 215 220Tyr Leu Ala Ser Pro Ile Met Val Ile Ala Tyr Ala Ile Ala Gly Thr225 230 235 240Met Asp Phe Asp Phe Glu Asn Glu Ala Leu Gly Gln Asp Gln Asp Gly
245 250 255Asn Asp Val Phe Leu Lys Asp Ile Trp Pro Ser Thr Glu Glu Ile Glu
260 265 270Asp Thr Ile Gln Gln Ala Ile Ser Arg Glu Leu Tyr Glu Ala Asp Tyr
275 280 285Ala Asp Val Phe Lys Gly Asp Lys Gln Trp Gln Glu Leu Asp Val Pro
290 295 300Thr Gly Asp Thr Phe Glu Trp Asp Glu Asn Ser Thr Tyr Ile Arg Lys305 310 315 320Ala Pro Tyr Phe Asp Gly Met Pro Val Glu Pro Val Ala Val Thr Asp
325 330 335Ile Gln Gly Ala Arg Val Leu Ala Lys Leu Gly Asp Ser Val Thr Thr
340 345 350Asp His Ile Ser Pro Ala Ser Ser Ile Lys Pro Gly Thr Pro Ala Ala
355 360 365Gln Tyr Leu Asp Glu His Gly Val Glu Arg His Asp Tyr Asn Ser Leu
370 375 380Gly Ser Arg Arg Gly Asn His Glu Val Met Met Arg Gly Thr Phe Ala385 390 395 400Asn Ile Arg Leu Gln Asn Gln Leu Val Asp Ile Ala Gly Gly Tyr Thr
405 410 415Arg Asp Phe Thr Gln Glu Gly Ala Pro Gln Ala Phe Ile Tyr Asp Ala
420 425 430Ser Val Asn Tyr Lys Ala Ala Gly Ile Pro Leu Val Val Leu Gly Gly
435 440 445Lys Glu Tyr Gly Thr Gly Ser Ser Arg Asp Trp Ala Ala Lys Gly Thr
450 455 460Asn Leu Leu Gly Ile Arg Ala Val Ile Thr Glu Ser Phe Glu Arg Ile465 470 475 480His Arg Ser Asn Leu Ile Gly Met Gly Val Val Pro Leu Gln Phe Pro
485 490 495Ala Gly Glu Ser His Glu Ser Leu Gly Leu Asp Gly Thr Glu Thr Phe
500 505 510Asp Ile Thr Gly Leu Thr Ala Leu Asn Glu Gly Glu Thr Pro Lys Thr
515 520 525Val Lys Val Thr Ala Thr Lys Glu Asn Gly Asp Val Val Glu Phe Asp
530 535 540Ala Ile Cys Pro His Arg His Pro Arg545 550<210>557<211>874<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(874)<223>RXN02213<400>557ttctgtggaa tgagaatccg atgtttttct cacgccggct cagccgaagc agacgccgtc 60gcgaaatctc accctaaaaa agttagaatt ggagctcact gtg act gaa agc aag 115
Val Thr Glu Ser Lys
1 5aac tcc ttc aat gct aag agc acc ctt gaa gtt ggc gac aag tcc tat 163Asn Ser Phe Asn Ala Lys Ser Thr Leu Glu Val Gly Asp Lys Ser Tyr
10 15 20gac tac ttc gcc ctc tct gca gtg cct ggc atg gag aag ctg ccg tac 211Asp Tyr Phe Ala Leu Ser Ala Val Pro Gly Met Glu Lys Leu Pro Tyr
25 30 35tcc ctc aag gtt ctc gga gag aac ctt ctt cgt acc gaa gac ggc gca 259Ser Leu Lys Val Leu Gly Glu Asn Leu Leu Arg Thr Glu Asp Gly Ala
40 45 50aac atc acc aac gag cac att gag gct atc gcc aac tgg gat gca tct 307Asn Ile Thr Asn Glu His Ile Glu Ala Ile Ala Asn Trp Asp Ala Ser
55 60 65tcc gat cca agc atc gaa atc cag ttc acc cca gcc cgt gtt ctc atg 355Ser Asp Pro Ser Ile Glu Ile Gln Phe Thr Pro Ala Arg Val Leu Met70 75 80 85cag gac ttc acc ggt gtc cct tgt gta gtt gac ctc gca acc atg cgt 403Gln Asp Phe Thr Gly Val Pro Cys Val Val Asp Leu Ala Thr Met Arg
90 95 100gag gca gtt gct gca ctc ggt ggc gac cct aac gac gtc aac cca ctg 451Glu Ala Val Ala Ala Leu Gly Gly Asp Pro Asn Asp Val Asn Pro Leu
105 110 115aac cca gcc gag atg gtc att gac cac tcc gtc atc gtg gag gct ttc 499Asn Pro Ala Glu Met Val Ile Asp His Ser Val Ile Val Glu Ala Phe
120 125 130ggc cgc cca gat gca ctg gct aag aac gtt gag atc gag tac gag cgc 547Gly Arg Pro Asp Ala Leu Ala Lys Asn Val Glu Ile Glu Tyr Glu Arg
135 140 145aac gag gag cgt tac cag ttc ctg cgt tgg ggt tcc gag tcc ttc tcc 595Asn Glu Glu Arg Tyr Gln Phe Leu Arg Trp Gly Ser Glu Ser Phe Ser150 155 160 165aac ttc cgc gtt gtt cct cca gga acc ggt atc gtc cac cag gtc aac 643Asn Phe Arg Val Val Pro Pro Gly Thr Gly Ile Val His Gln Val Asn
170 175 180att gag tac ttg gct cgc gtc gtc ttc gac aac gag ggc ctt gca tac 691Ile Glu Tyr Leu Ala Arg Val Val Phe Asp Asn Glu Gly Leu Ala Tyr
185 190 195cca gat acc tgc atc ggt acc gac tcc cac acc acc atg gaa aac ggc 739Pro Asp Thr Cys Ile Gly Thr Asp Ser His Thr Thr Met Glu Asn Gly
200 205 210ctg ggc atc ctg ggc tgg ggc gtt ggt ggc att gag gct gaa gca gca 787Leu Gly Ile Leu Gly Trp Gly Val Gly Gly Ile Glu Ala Glu Ala Ala
215 220 225atg ctc ggc cag cca gtg tcc atg ctg atc cct cgc gtt gtt ggc ttc 835Met Leu Gly Gln Pro Val Ser Met Leu Ile Pro Arg Val Val Gly Phe230 235 240 245aag ttg acc ggc gag atc cca gta ggc gtt acc gca act 874Lys Leu Thr Gly Glu Ile Pro Val Gly Val Thr Ala Thr
250 255<210>558<211>258<212>PRT<213>谷氨酸棒杆菌<400>558Val Thr Glu Ser Lys Asn Ser Phe Asn Ala Lys Ser Thr Leu Glu Val1 5 10 15Gly Asp Lys Ser Tyr Asp Tyr Phe Ala Leu Ser Ala Val Pro Gly Met
20 25 30Glu Lys Leu Pro Tyr Ser Leu Lys Val Leu Gly Glu Asn Leu Leu Arg
35 40 45Thr Glu Asp Gly Ala Asn Ile Thr Asn Glu His Ile Glu Ala Ile Ala
50 55 60Asn Trp Asp Ala Ser Ser Asp Pro Ser Ile Glu Ile Gln Phe Thr Pro65 70 75 80Ala Arg Val Leu Met Gln Asp Phe Thr Gly Val Pro Cys Val Val Asp
85 90 95Leu Ala Thr Met Arg Glu Ala Val Ala Ala Leu Gly Gly Asp Pro Asn
100 105 110Asp Val Asn Pro Leu Asn Pro Ala Glu Met Val Ile Asp His Ser Val
115 120 125Ile Val Glu Ala Phe Gly Arg Pro Asp Ala Leu Ala Lys Asn Val Glu
130 135 140Ile Glu Tyr Glu Arg Asn Glu Glu Arg Tyr Gln Phe Leu Arg Trp Gly145 150 155 160Ser Glu Ser Phe Ser Asn Phe Arg Val Val Pro Pro Gly Thr Gly Ile
165 170 175Val His Gln Val Asn Ile Glu Tyr Leu Ala Arg Val Val Phe Asp Asn
180 185 190Glu Gly Leu Ala Tyr Pro Asp Thr Cys Ile Gly Thr Asp Ser His Thr
195 200 205Thr Met Glu Asn Gly Leu Gly Ile Leu Gly Trp Gly Val Gly Gly Ile
210 215 220Glu Ala Glu Ala Ala Met Leu Gly Gln Pro Val Ser Met Leu Ile Pro225 230 235 240Arg Val Val Gly Phe Lys Leu Thr Gly Glu Ile Pro Val Gly Val Thr
245 250 255Ala Thr<210>559<211>817<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(817)<223>FRXA02213<400>559ttctgtggaa tgagaatccg atgtttttct cacgccggct cagccgaagc agacgccgtc 60gcgaaatctc accctaaaaa agttagaatt ggagctcact gtg act gaa agc aag 115
Val Thr Glu Ser Lys
1 5aac tcc ttc aat gct aag agc acc ctt gaa gtt ggc gac aag tcc tat 163Asn Ser Phe Asn Ala Lys Ser Thr Leu Glu Val Gly Asp Lys Ser Tyr
10 15 20gac tac ttc gcc ctc tct gca gtg cct ggc atg gag aag ctg ccg tac 211Asp Tyr Phe Ala Leu Ser Ala Val Pro Gly Met Glu Lys Leu Pro Tyr
25 30 35tcc ctc aag gtt ctc gga gag aac ctt ctt cgt acc gaa gac ggc gca 259Ser Leu Lys Val Leu Gly Glu Asn Leu Leu Arg Thr Glu Asp Gly Ala
40 45 50aac atc acc aac gag cac att gag gct atc gcc aac tgg gat gca tct 307Asn Ile Thr Asn Glu His Ile Glu Ala Ile Ala Asn Trp Asp Ala Ser
55 60 65tcc gat cca agc atc gaa atc cag ttc acc cca gcc cgt gtt ctc atg 355Ser Asp Pro Ser Ile Glu Ile GIn Phe Thr Pro Ala Arg Val Leu Met70 75 80 85cag gac ttc acc ggt gtc cct tgt gta gtt gac ctc gca acc atg cgt 403Gln Asp Phe Thr Gly Val Pro Cys Val Val Asp Leu Ala Thr Met Arg
90 95 100gag gca gtt gct gca ctc ggt ggc gac cct aac gac gtc aac cca ctg 451Glu Ala Val Ala Ala Leu Gly Gly Asp Pro Asn Asp Val Asn Pro Leu
105 110 115aac cca gcc gag atg gtc att gac cac tcc gtc atc gtg gag gct ttc 499Asn Pro Ala Glu Met Val Ile Asp His Ser Val Ile Val Glu Ala Phe
120 125 130ggc cgc cca gat gca ctg gct aag aac gtt gag atc gag tac gag cgc 547Gly Arg Pro Asp Ala Leu Ala Lys Asn Val Glu Ile Glu Tyr Glu Arg
135 140 145aac gag gag cgt tac cag ttc ctg cgt tgg ggt tcc gag tcc ttc tcc 595Asn Glu Glu Arg Tyr Gln Phe Leu Arg Trp Gly Ser Glu Ser Phe Ser150 155 160 165aac ttc cgc gtt gtt cct cca gga acc ggt atc gtc cac cag gtc aac 643Asn Phe Arg Val Val Pro Pro Gly Thr Gly Ile Val His Gln Val Asn
170 175 180att gag tac ttg gct cgc gtc gtc ttc gac aac gag ggc ctt gca tac 691Ile Glu Tyr Leu Ala Arg Val Val Phe Asp Asn Glu Gly Leu Ala Tyr
185 190 195cca gat acc tgc atc ggt acc gac tcc cac acc acc atg gaa aac ggc 739Pro Asp Thr Cys Ile Gly Thr Asp Ser His Thr Thr Met Glu Asn Gly
200 205 210ctg ggc atc ctg ggc tgg ggc gtt ggt ggc att gag gct gaa gca gca 787Leu Gly Ile Leu Gly Trp Gly Val Gly Gly Ile Glu Ala Glu Ala Ala
215 220 225atg ctc ggc cag cca gtg tcc atg ctg atc 817Met Leu Gly Gln Pro Val Ser Met Leu Ile230 235<210>560<211>239<212>PRT<213>谷氨酸棒杆菌<400>560Val Thr Glu Ser Lys Asn Ser Phe Asn Ala Lys Ser Thr Leu Glu Val1 5 10 15Gly Asp Lys Ser Tyr Asp Tyr Phe Ala Leu Ser Ala Val Pro Gly Met
20 25 30Glu Lys Leu Pro Tyr Ser Leu Lys Val Leu Gly Glu Asn Leu Leu Arg
35 40 45Thr Glu Asp Gly Ala Asn Ile Thr Asn Glu His Ile Glu Ala Ile Ala
50 55 60Asn Trp Asp Ala Ser Ser Asp Pro Ser Ile Glu Ile Gln Phe Thr Pro65 70 75 80Ala Arg Val Leu Met Gln Asp Phe Thr Gly Val Pro Cys Val Val Asp
85 90 95Leu Ala Thr Met Arg Glu Ala Val Ala Ala Leu Gly Gly Asp Pro Asn
100 105 110Asp Val Asn Pro Leu Asn Pro Ala Glu Met Val Ile Asp His Ser Val
115 120 125Ile Val Glu Ala Phe Gly Arg Pro Asp Ala Leu Ala Lys Asn Val Glu130 135 140Ile Glu Tyr Glu Arg Asn Glu Glu Arg Tyr Gln Phe Leu Arg Trp Gly145 150 155 160Ser Glu Ser Phe Ser Asn Phe Arg Val Val Pro Pro Gly Thr Gly Ile
165 170 175Val His Gln Val Asn Ile Glu Tyr Leu Ala Arg Val Val Phe Asp Asn
180 185 190Glu Gly Leu Ala Tyr Pro Asp Thr Cys Ile Gly Thr Asp Ser His Thr
195 200 205Thr Met Glu Asn Gly Leu Gly Ile Leu Gly Trp Gly Val Gly Gly Ile
210 215 220Glu Ala Glu Ala Ala Met Leu Gly Gln Pro Val Ser Met Leu Ile225 230 235<210>561<211>2891<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2868)<223>RXA02056<400>561cgc cac tct gtt cca cgt cta acc aag ggc cag ggc acc atc atc ggt 48Arg His Ser Val Pro Arg Leu Thr Lys Gly Gln Gly Thr Ile Ile Gly1 5 10 15gtc ggt tcc atg gat tac cca gca gag ttc cag ggc gct tca gaa gac 96Val Gly Ser Met Asp Tyr Pro Ala Glu Phe Gln Gly Ala Ser Glu Asp
20 25 30cgc ctt gca gag ctc ggc gtt ggc aaa ctt gtc acc atc acc tcc acc 144Arg Leu Ala Glu Leu Gly Val Gly Lys Leu Val Thr Ile Thr Ser Thr
35 40 45tac gat cac cgc gtg atc cag ggt gct gtg tcc ggt gaa ttc ctg cgc 192Tyr Asp His Arg Val Ile Gln Gly Ala Val Ser Gly Glu Phe Leu Arg
50 55 60acc atg tct cgc ctg ctc acc gat gat tcc ttc tgg gat gag atc ttc 240Thr Met Ser Arg Leu Leu Thr Asp Asp Ser Phe Trp Asp Glu Ile Phe65 70 75 80gac gca atg aac gtt cct tac acc cca atg cgt tgg gca cag gac gtt 288Asp Ala Met Asn Val Pro Tyr Thr Pro Met Arg Trp Ala Gln Asp Val
85 90 95cca aac acc ggt gtt gat aag aac acc cgc gtc atg cag ctc att gag 336Pro Asn Thr Gly Val Asp Lys Asn Thr Arg Val Met Gln Leu Ile Glu
100 105 110gca tac cgc tcc cgt gga cac ctc atc gct gac acc aac cca ctt tca 384Ala Tyr Arg Ser Arg Gly His Leu Ile Ala Asp Thr Asn Pro Leu Ser
115 120 125tgg gtt cag cct ggc atg cca gtt cca gac cac cgc gac ctc gac atc 432Trp Val Gln Pro Gly Met Pro Val Pro Asp His Arg Asp Leu Asp Ile
130 135 140gag acc cac aac ctg acc atc tgg gat ctg gac cgt acc ttc aac gtc 480Glu Thr His Asn Leu Thr Ile Trp Asp Leu Asp Arg Thr Phe Asn Val145 150 155 160ggt ggc ttc ggc ggc aag gag acc atg acc ctg cgc gag gta ctg tcc 528Gly Gly Phe Glu Gly Lys Glu Thr Met Thr Leu Arg Glu Val Leu Ser
165 170 175cgc ctc cgc gct gcg tac acc ctc aag gtc ggc tcc gaa tac acc cac 576Arg Leu Arg Ala Ala Tyr Thr Leu Lys Val Gly Ser Glu Tyr Thr His
180 185 190atc ctg gac cgc gac gag cgc acc tgg ctg cag gac cgc ctc gag gcc 624Ile Leu Asp Arg Asp Glu Arg Thr Trp Leu Gln Asp Arg Leu Glu Ala
195 200 205gga atg cca aag cca acc cag gca gag cag aag tac atc ctg cag aag 672Gly Met Pro Lys Pro Thr Gln Ala Glu Gln Lys Tyr Ile Leu Gln Lys
210 215 220ctg aac gcc gcg gag gct ttc gag aac ttc ctg cag acc aag tac gtc 720Leu Asn Ala Ala Glu Ala Phe Glu Asn Phe Leu Gln Thr Lys Tyr Val225 230 235 240ggc cag aag cgc ttc tcc ctc gaa ggt gca gaa gca ctt atc cca ctg 768Gly Gln Lys Arg Phe Ser Leu Glu Gly Ala Glu Ala Leu Ile Pro Leu
245 250 255atg gac tcc gcc atc gac acc gcc gca ggc caa ggc ctc gac gaa gtt 816Met Asp Ser Ala Ile Asp Thr Ala Ala Gly Gln Gly Leu Asp Glu Val
260 265 270gtc atc ggt atg cca cac cgt ggt cgc ctc aac gtg ctg ttc aac atc 864Val Ile Gly Met Pro His Arg Gly Arg Leu Asn Val Leu Phe Asn Ile
275 280 285gtg ggc aag cca ctg gca tcc atc ttc aac gag ttt gaa ggc caa atg 912Val Gly Lys Pro Leu Ala Ser Ile Phe Asn Glu Phe Glu Gly Gln Met
290 295 300gag cag ggc cag atc ggt ggc tcc ggt gac gtg aag tac cac ctc ggt 960Glu Gln Gly Gln Ile Gly Gly Ser Gly Asp Val Lys Tyr His Leu Gly305 310 315 320tcc gaa ggc cag cac ctg cag atg ttc ggc gac ggc gag atc aag gtc 1008Ser Glu Gly Gln His Leu Gln Met Phe Gly Asp Gly Glu Ile Lys Val
325 330 335tcc ctg act gct aac ccg tcc cac ctg gaa gct gtt aac cca gcg atg 1056Ser Leu Thr Ala Asn Pro Ser His Leu Glu Ala Val Asn Pro Ala Met
340 345 350gaa ggt att gtc cgc gca aag cag gac tac ctg gac aag ggc gta gac 1104Glu Gly Ile Val Arg Ala Lys Gln Asp Tyr Leu Asp Lys Gly Val Asp
355 360 365ggc aag act gtt gtg cca ctg ctg ctc cac ggt gac gct gca ttc gca 1152Gly Lys Thr Val Val Pro Leu Leu Leu His Gly Asp Ala Ala Phe Ala
370 375 380ggc ctg ggc atc gtg cca gaa acc atc aac ctg gct aag ctg cgt ggc 1200Gly Leu Gly Ile Val Pro Glu Thr Ile Asn Leu Ala Lys Leu Arg Gly385 390 395 400tac gac gtc ggc ggc acc atc cac atc gtg gtg aac aac cag atc ggc 1248Tyr Asp Val Gly Gly Thr Ile His Ile Val Val Asn Asn Gln Ile Gly
405 410 415ttc acc acc acc cca gac tcc agc cgc tcc atg cac tac gca acc gac 1296Phe Thr Thr Thr Pro Asp Ser Ser Arg Ser Met His Tyr Ala Thr Asp
420 425 430tac gcc aag gca ttc ggc tgc cca gtc ttc cac gtc aac ggc gac gac 1344Tyr Ala Lys Ala Phe Gly Cys Pro Val Phe His Val Asn Gly Asp Asp
435 440 445cca gag gca gtt gtc tgg gtt ggc cag ctg gcc acc gag tac cgt cgt 1392Pro Glu Ala Val Val Trp Val Gly Gln Leu Ala Thr Glu Tyr Arg Arg
450 455 460cgc ttc ggc aag gac gtc ttc atc gac ctc gtc tgc tac cgc ctc cgc 1440Arg Phe Gly Lys Asp Val Phe Ile Asp Leu Val Cys Tyr Arg Leu Arg465 470 475 480ggc cac aac gaa gct gat gat cct tcc atg acc cag cca aag atg tat 1488Gly His Asn Glu Ala Asp Asp Pro Ser Met Thr Gln Pro Lys Met Tyr
485 490 495gag ctc atc acc ggc cgc gag acc gtt cgt gct cag tac acc gsa gac 1536Glu Leu Ile Thr Gly Arg Glu Thr Val Arg Ala Gln Tyr Thr Glu Asp
500 505 510ctg ctc gga cgt gga gac ctc tcc aac gaa gat gca gaa gca gtc gtc 1584Leu Leu Gly Arg Gly Asp Leu Ser Asn Glu Asp Ala Glu Ala Val Val
515 520 525cgc gac ttc cac gac cag atg gaa tct gtg ttc aac gaa gtc aag gaa 1632Arg Asp Phe His Asp Gln Met Glu Ser Val Phe Asn Glu Val Lys Glu
530 535 540ggc ggc aag aag cag gct gag gca cag acc ggc atc acc ggc tcc cag 1680Gly Gly Lys Lys Gln Ala Glu Ala Gln Thr Gly Ile Thr Gly Ser Gln545 550 555 560aag ctt cca cac ggc ctt gag acc aac atc tcc cgt gaa gag ctc ctg 1728Lys Leu Pro His Gly Leu Glu Thr Asn Ile Ser Arg Glu Glu Leu Leu
565 570 575gaa ctg gga cag gct ttc gcc aac acc cca gaa ggc ttc aac tac cac 1776Glu Leu Gly Gln Ala Phe Ala Asn Thr Pro Glu Gly Phe Asn Tyr His
580 585 590cca cgt gtg gct ccc gtt gct aag aag cgc gtc tcc tct gtc acc gaa 1824Pro Arg Val Ala Pro Val Ala Lys Lys Arg Val Ser Ser Val Thr Glu
595 600 605ggt ggc atc gac tgg gca tgg ggc gag ctc ctc gcc ttc ggt tcc ctg 1872Gly Gly Ile Asp Trp Ala Trp Gly Glu Leu Leu Ala Phe Gly Ser Leu
610 615 620gct aac tcc ggc cgc ttg gtt cgc ctt gca ggt gaa gat tcc cgc cgc 1920Ala Asn Scr Gly Arg Leu Val Arg Leu Ala Gly Glu Asp Ser Arg Arg625 630 635 640ggt acc ttc acc cag cgc cac gca gtt gcc atc gac cca gcg acc gct 1968Gly Thr Phe Thr Gln Arg His Ala Val Ala Ile Asp Pro Ala Thr Ala
645 650 655gaa gag ttc aac cca ctc cac gag ctt gca cag tcc aag ggc aac aac 2016Glu Glu Phe Asn Pro Leu His Glu Leu Ala Gln Ser Lys Gly Asn Asn
660 665 670ggt aag ttc ctg gtc tac aac tcc gca ctg acc gag tac gca ggc atg 2064Gly Lys Phe Leu Val Tyr Asn Ser Ala Leu Thr Glu Tyr Ala Gly Met
675 680 685ggc ttc gag tac ggc tac tcc gta gga aac gaa gac tcc atc gtt gca 2112Gly Phe Glu Tyr Gly Tyr Ser Val Gly Asn Glu Asp Ser Ile Val Ala
690 695 700tgg gaa gca cag ttc ggc gac ttc gcc aac ggc gct cag acc atc atc 2160Trp Glu Ala Gln Phe Gly Asp Phe Ala Asn Gly Ala Gln Thr Ile Ile705 710 715 720gat gag tac gtc tcc tca ggc gaa gct aag tgg ggc cag acc tcc aag 2208Asp Glu Tyr Val Ser Ser Gly Glu Ala Lys Trp Gly Gln Thr Ser Lys
725 730 735ctg atc ctt ctg ctg cct cac ggc tac gaa ggc cag ggc cca gac cac 2256Leu Ile Leu Leu Leu Pro His Gly Tyr Glu Gly Gln Gly Pro Asp His
740 745 750tct tcc gca cgt atc gag cgc ttc ctg cag ctg tgc gct gag ggt tcc 2304Ser Ser Ala Arg Ile Glu Arg Phe Leu Gln Leu Cys Ala Glu Gly Ser
755 760 765atg act gtt gct cag cca tcc acc cca gca aac cac ttc cac cta ctg 2352Met Thr Val Ala Gln Pro Ser Thr Pro Ala Asn His Phe His Leu Leu
770 775 780cgt cgt cac gct ctg tcc gac ctg aag cgt cca ctg gtt atc ttc acc 2400Arg Arg His Ala Leu Ser Asp Leu Lys Arg Pro Leu Val Ile Phe Thr785 790 795 800ccg aag tcc atg ctg cgt aac aag gct gct gcc tcc gca cca gaa gac 2448Pro Lys Ser Met Leu Arg Asn Lys Ala Ala Ala Ser Ala Pro Glu Asp
805 810 815ttc act gag gtc acc aag ttc cag tcc gtg atc aac gat cca aac gtt 2496Phe Thr Glu Val Thr Lys Phe Gln Ser Val Ile Asn Asp Pro Asn Val
820 825 830gca gat gca gcc aag gtg aag aag gtc atg ctg gtc tcc ggc aag ctg 2544Ala Asp Ala Ala Lys Val Lys Lys Val Met Leu Val Ser Gly Lys Leu
835 840 845tac tac gaa ttg gca aag cgc aag gag aag gac gga cgc gac gac atc 2592Tyr Tyr Glu Leu Ala Lys Arg Lys Glu Lys Asp Gly Arg Asp Asp Ile
850 855 860gcg atc gtt cgt atc gaa atg ctc cac cca att ccg ttc aac cgc atc 2640Ala Ile Val Arg Ile Glu Met Leu His Pro Ile Pro Phe Asn Arg Ile865 870 875 880tcc gag gct ctt gcc ggc tac cct aac gct gag gaa gtc ctc ttc gtt 2688Ser Glu Ala Leu Ala Gly Tyr Pro Asn Ala Glu Glu Val Leu Phe Val
885 890 895cag gat gag cca gca aac cag ggc cca tgg ccg ttc tac cag gag cac 2736Gln Asp Glu Pro Ala Asn Gln Gly Pro Trp Pro Phe Tyr Gln Glu His
900 905 910ctc cca gag ctg atc ccg aac atg cca aag atg cgc cgc gtt tcc cgc 2784Leu Pro Glu Leu Ile Pro Asn Met Pro Lys Met Arg Arg Val Ser Arg
915 920 925cgc gct cag tcc tcc acc gca act ggt gtt gcc aag gtg cac cag ctg 2832Arg Ala Gln Ser Ser Thr Ala Thr Gly Val Ala Lys Val His Gln Leu
930 935 940gag gag aag cag ctt atc gac gag gct ttc gag gct taagtcttta 2878Glu Glu Lys Gln Leu Ile Asp Glu Ala Phe Glu Ala945 950 955tagtcctgca cta 2891<210>562<211>956<212>PRT<213>谷氨酸棒杆菌<400>562Arg His Ser Val Pro Arg Leu Thr Lys Gly Gln Gly Thr Ile Ile Gly1 5 10 15Val Gly Ser Met Asp Tyr Pro Ala Glu Phe Gln Gly Ala Ser Glu Asp
20 25 30Arg Leu Ala Glu Leu Gly Val Gly Lys Leu Val Thr Ile Thr Ser Thr
35 40 45Tyr Asp His Arg Val Ile Gln Gly Ala Val Ser Gly Glu Phe Leu Arg
50 55 60Thr Met Ser Arg Leu Leu Thr Asp Asp Ser Phe Trp Asp Glu Ile Phe65 70 75 80Asp Ala Met Asn Val Pro Tyr Thr Pro Met Arg Trp Ala Gln Asp Val
85 90 95Pro Asn Thr Gly Val Asp Lys Asn Thr Arg Val Met Gln Leu Ile Glu
100 105 110Ala Tyr Arg Ser Arg Gly His Leu Ile Ala Asp Thr Asn Pro Leu Ser
115 120 125Trp Val Gln Pro Gly Met Pro Val Pro Asp His Arg Asp Leu Asp Ile
130 135 140Glu Thr His Asn Leu Thr Ile Trp Asp Leu Asp Arg Thr Phe Asn Val145 150 155 160Gly Gly Phe Gly Gly Lys Glu Thr Met Thr Leu Arg Glu Val Leu Ser
165 170 175Arg Leu Arg Ala Ala Tyr Thr Leu Lys Val Gly Ser Glu Tyr Thr His
180 185 190Ile Leu Asp Arg Asp Glu Arg Thr Trp Leu Gln Asp Arg Leu Glu Ala
195 200 205Gly Met Pro Lys Pro Thr Gln Ala Glu Gln Lys Tyr Ile Leu Gln Lys
210 215 220Leu Asn Ala Ala Glu Ala Phe Glu Asn Phe Leu Gln Thr Lys Tyr Val225 230 235 240Gly Gln Lys Arg Phe Ser Leu Glu Gly Ala Glu Ala Leu Ile Pro Leu
245 250 255Met Asp Ser Ala Ile Asp Thr Ala Ala Gly Gln Gly Leu Asp Glu Val
260 265 270Val Ile Gly Met Pro His Arg Gly Arg Leu Asn Val Leu Phe Asn Ile
275 280 285Val Gly Lys Pro Leu Ala Ser Ile Phe Asn Glu Phe Glu Gly Gln Met
290 295 300Glu Gln Gly Gln Ile Gly Gly Ser Gly Asp Val Lys Tyr His Leu Gly305 310 315 320Ser Glu Gly Gln His Leu Gln Met Phe Gly Asp Gly Glu Ile Lys Val
325 330 335Ser Leu Thr Ala Asn Pro Ser His Leu Glu Ala Val Asn Pro Ala Met
340 345 350Glu Gly Ile Val Arg Ala Lys Gln Asp Tyr Leu Asp Lys Gly Val Asp
355 360 365Gly Lys Thr Val Val Pro Leu Leu Leu His Gly Asp Ala Ala Phe Ala
370 375 380Gly Leu Gly Ile Val Pro Glu Thr Ile Asn Leu Ala Lys Leu Arg Gly385 390 395 400Tyr Asp Val Gly Gly Thr Ile His Ile Val Val Asn Asn Gln Ile Gly
405 410 415Phe Thr Thr Thr Pro Asp Ser Ser Arg Ser Met His Tyr Ala Thr Asp
420 425 430Tyr Ala Lys Ala Phe Gly Cys Pro Val Phe His Val Asn Gly Asp Asp
435 440 445Pro Glu Ala Val Val Trp Val Gly Gln Leu Ala Thr Glu Tyr Arg Arg
450 455 460Arg Phe Gly Lys Asp Val Phe Ile Asp Leu Val Cys Tyr Arg Leu Arg465 470 475 480Gly His Asn Glu Ala Asp Asp Pro Ser Met Thr Gln Pro Lys Met Tyr
485 490 495Glu Leu Ile Thr Gly Arg Glu Thr Val Arg Ala Gln Tyr Thr Glu Asp
500 505 510Leu Leu Gly Arg Gly Asp Leu Ser Asn Glu Asp Ala Glu Ala Val Val
515 520 525Arg Asp Phe His Asp Gln Met Glu Ser Val Phe Asn Glu Val Lys Glu
530 535 540Gly Gly Lys Lys Gln Ala Glu Ala Gln Thr Gly Ile Thr Gly Ser Gln545 550 555 560Lys Leu Pro His Gly Leu Glu Thr Asn Ile Ser Arg Glu Glu Leu Leu
565 570 575Glu Leu Gly Gln Ala Phe Ala Asn Thr Pro Glu Gly Phe Asn Tyr His
580 585 590Pro Arg Val Ala Pro Val Ala Lys Lys Arg Val Ser Ser Val Thr Glu
595 600 605Gly Gly Ile Asp Trp Ala Trp Gly Glu Leu Leu Ala Phe Gly Ser Leu
610 615 620Ala Asn Ser Gly Arg Leu Val Arg Leu Ala Gly Glu Asp Ser Arg Arg625 630 635 640Gly Thr Phe Thr Gln Arg His Ala Val Ala Ile Asp Pro Ala Thr Ala
645 650 655Glu Glu Phe Asn Pro Leu His Glu Leu Ala Gln Ser Lys Gly Asn Asn
660 665 670Gly Lys Phe Leu Val Tyr Asn Ser Ala Leu Thr Glu Tyr Ala Gly Met
675 680 685Gly Phe Glu Tyr Gly Tyr Ser Val Gly Asn Glu Asp Ser Ile Val Ala
690 695 700Trp Glu Ala Gln Phe Gly Asp Phe Ala Asn Gly Ala Gln Thr Ile Ile705 710 715 720Asp Glu Tyr Val Ser Ser Gly Glu Ala Lys Trp Gly Gln Thr Ser Lys
725 730 735Leu Ile Leu Leu Leu Pro His Gly Tyr Glu Gly Gln Gly Pro Asp His
740 745 750Ser Ser Ala Arg Ile Glu Arg Phe Leu Gln Leu Cys Ala Glu Gly Ser
755 760 765Met Thr Val Ala Gln Pro Ser Thr Pro Ala Asn His Phe His Leu Leu
770 775 780Arg Arg His Ala Leu Ser Asp Leu Lys Arg Pro Leu Val Ile Phe Thr785 790 795 800Pro Lys Ser Met Leu Arg Asn Lys Ala Ala Ala Ser Ala Pro Glu Asp
805 810 815Phe Thr Glu Val Thr Lys Phe Gln Ser Val Ile Asn Asp Pro Asn Val
820 825 830Ala Asp Ala Ala Lys Val Lys Lys Val Met Leu Val Ser Gly Lys Leu
835 840 845Tyr Tyr Glu Leu Ala Lys Arg Lys Glu Lys Asp Gly Arg Asp Asp Ile
850 855 860Ala Ile Val Arg Ile Glu Met Leu His Pro Ile Pro Phe Asn Arg Ile865 870 875 880Ser Glu Ala Leu Ala Gly Tyr Pro Asn Ala Glu Glu Val Leu Phe Val
885 890 895Gln Asp Glu Pro Ala Asn Gln Gly Pro Trp Pro Phe Tyr Gln Glu His
900 905 910Leu Pro Glu Leu Ile Pro Asn Met Pro Lys Met Arg Arg Val Ser Arg
915 920 925Arg Ala Gln Ser Ser Thr Ala Thr Gly Val Ala Lys Val His Gln Leu
930 935 940Glu Glu Lys Gln Leu Ile Asp Glu Ala Phe Glu Ala945 950 955<210>563<211>1517<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1494)<223>RXA01745<400>563atc ctt gca gac gaa gac gac acc gtc gac gtc ggc gca gtc atc gcc 48Ile Leu Ala Asp Glu Asp Asp Thr Val Asp Val Gly Ala Val Ile Ala1 5 10 15cgc atc ggt gac gca aac gca gct gca gca cct gcc gaa gag gaa gca 96Arg Ile Gly Asp Ala Asn Ala Ala Ala Ala Pro Ala Glu Glu Glu Ala
20 25 30gct cct gcc gaa gag gaa gaa cca att aag gaa gag cca aag aag gag 144Ala Pro Ala Glu Glu Glu Glu Pro Val Lys Glu Glu Pro Lys Lys Glu
35 40 45gca gct cct gaa gct cca gca gca act ggc gcc gca acc gat gtg gaa 192Ala Ala Pro Glu Ala Pro Ala Ala Thr Gly Ala Ala Thr Asp Val Glu
50 55 60atg cca gaa ctc ggc gaa tcc gtc acc gaa ggc acc att acc cag tgg 240Met Pro Glu Leu Gly Glu Ser Val Thr Glu Gly Thr Ile Thr Gln Trp65 70 75 80ctc aag gct gtc ggc gac acc gtc gaa gta gac gaa cca ctt ctt gag 288Leu Lys Ala Val Gly Asp Thr Val Glu Val Asp Glu Pro Leu Leu Glu
85 90 95gtc tcc acc gac aag gtc gac acc gaa atc cca tcc cca gta gca ggc 336Val Ser Thr Asp Lys Val Asp Thr Glu Ile Pro Ser Pro Val Ala Gly
100 105 110acc atc gtg gag atc ctt gca gac gaa gac gac acc gtc gac gtc ggc 384Thr Ile Val Glu Ile Leu Ala Asp Glu Asp Asp Thr Val Asp Val Gly
115 120 125gca gtc atc gcc cgc atc ggt gac gca aac gca gct gca gca cct gcc 432Ala Val Ile Ala Arg Ile Gly Asp Ala Asn Ala Ala Ala Ala Pro Ala
130 135 140gaa gag gaa gca gct cct gcc gaa gag gag gaa cca gtt aag gaa gag 480Glu Glu Glu Ala Ala Pro Ala Glu Glu Glu Glu Pro Val Lys Glu Glu145 150 155 160cca aag aag gaa gag ccc aag aag gaa gag ccc aag aag gaa gca gct 528Pro Lys Lys Glu Glu Pro Lys Lys Glu Glu Pro Lys Lys Glu Ala Ala
165 170 175act aca cct gct gcg gca tcc gca act gtg tcc gct tct ggc gac aac 576Thr Thr Pro Ala Ala Ala Ser Ala Thr Val Ser Ala Ser Gly Asp Asn
180 185 190gtt cca tac gtc acc cca ctg gtg cgc aag crt gct gaa aag cac ggc 624Val Pro Tyr Val Thr Pro Leu Val Arg Lys Leu Ala Glu Lys His Gly
195 200 205gtt gac ttg aac acc gtg acc ggt acc ggt atc ggt ggc cgt atc cgc 672Val Asp Leu Asn Thr Val Thr Gly Thr Gly Ile Gly Gly Arg Ile Arg
210 215 220aag cag gat gtt ttg gct gct gcg aac ggc gag gct gca cct gct gag 720Lys Gln Asp Val Leu Ala Ala Ala Asn Gly Glu Ala Ala Pro Ala Glu225 230 235 240gct gct gct cct gtt tcc gct tgg tcc act aag tct gtt gac cct gag 768Ala Ala Ala Pro Val Ser Ala Trp Ser Thr Lys Ser Val Asp Pro Glu
245 250 255aag gct aag ctc cgt ggt acc act cag aag gtc aac cgc atc cgt gag 816Lys Ala Lys Leu Arg Gly Thr Thr Gln Lys Val Asn Arg Ile Arg Glu
260 265 270atc acc gcg atg aag acc gtc gag gct ctg cag att tct gct cag ctc 864Ile Thr Ala Met Lys Thr Val Glu Ala Leu Gln Ile Ser Ala Gln Leu
275 280 285acc cag ctg cac gag gtc gat atg act cgc gtt gct gag ctg cgt aag 912Thr Gln Leu His Glu Val Asp Met Thr Arg Val Ala Glu Leu Arg Lys
290 295 300aag aac aag ccc gcg ttc atc gag aag cac ggt gtg aac ctc act tac 960Lys Asn Lys Pro Ala Phe Ile Glu Lys His Gly Val Asn Leu Thr Tyr305 310 315 320ctg cca ttc ttc gtg aag gca gtt gtc gag gct ttg gtt tcc cat cca 1008Leu Pro Phe Phe Val Lys Ala Val Val Glu Ala Leu Val Ser His Pro
325 330 335aac gtc aac gcg tct ttc aac gcg aag acc aag gag atg acc tac cac 1056Asn Val Asn Ala Ser Phe Asn Ala Lys Thr Lys Glu Met Thr Tyr His
340 345 350tcc tcc gtt aac ctc tcc atc gct gtt gat acc cca gct ggt ctg ttg 1104Ser Ser Val Asn Leu Ser Ile Ala Val Asp Thr Pro Ala Gly Leu Leu
355 360 365acc cca gtc att cac gat gct cag gat ctc tcc atc cca gag atc gca 1152Thr Pro Val Ile His Asp Ala Gln Asp Leu Ser Ile Pro Glu Ile Ala
370 375 380aag gca att gtt gac ctg gct gat cgt tca cgc aac aac aag ctg aag 1200Lys Ala Ile Val Asp Leu Ala Asp Arg Ser Arg Asn Asn Lys Leu Lys385 390 395 400cca aac gat ctg tcc ggt ggc acc ttc acc atc acc aac att ggt tct 1248Pro Asn Asp Leu Ser Gly Gly Thr Phe Thr Ile Thr Asn Ile Gly Ser
405 410 415gaa ggc gca ctg tct gat acc cca atc ctg gtt cca cca cag gct ggc 1296Glu Gly Ala Leu Ser Asp Thr Pro Ile Leu Val Pro Pro Gln Ala Gly
420 425 430atc ttg ggc acc ggc gcg atc gtg aag cgt cca gtt gtc atc acc gag 1344Ile Leu Gly Thr Gly Ala Ile Val Lys Arg Pro Val Val Ile Thr Glu
435 440 445gat gga att gat tcc atc gcg atc cgt cag atg gtc ttc cta cca ctg 1392Asp Gly Ile Asp Ser Ile Ala Ile Arg Gln Met Val Phe Leu Pro Leu
450 455 460acc tac gac cac cag gtt gta gat ggc gca gat gct ggt cgc ttc ctg 1440Thr Tyr Asp His Gln Val Val Asp Gly Ala Asp Ala Gly Arg Phe Leu465 470 475 480acc acc atc aag gac cgc ctt gag acc gct aac ttc gaa ggc gat ctg 1488Thr Thr Ile Lys Asp Arg Leu Glu Thr Ala Asn Phe Glu Gly Asp Leu
485 490 495cag ctc taagatctct gcaagttaaa acc 1517Gln Leu<210>564<211>498<212>PRT<213>谷氨酸棒杆菌<400>564Ile Leu Ala Asp Glu Asp Asp Thr Val Asp Val Gly Ala Val Ile Ala1 5 10 15Arg Ile Gly Asp Ala Asn Ala Ala Ala Ala Pro Ala Glu Glu Glu Ala
20 25 30Ala Pro Ala Glu Glu Glu Glu Pro Val Lys Glu Glu Pro Lys Lys Glu
35 40 45Ala Ala Pro Glu Ala Pro Ala Ala Thr Gly Ala Ala Thr Asp Val Glu
50 55 60Met Pro Glu Leu Gly Glu Ser Val Thr Glu Gly Thr Ile Thr Gln Trp65 70 75 80Leu Lys Ala Val Gly Asp Thr Val Glu Val Asp Glu Pro Leu Leu Glu
85 90 95Val Ser Thr Asp Lys Val Asp Thr Glu Ile Pro Ser Pro Val Ala Gly
100 105 110Thr Ile Val Glu Ile Leu Ala Asp Glu Asp Asp Thr Val Asp Val Gly
115 120 125Ala Val Ile Ala Arg Ile Gly Asp Ala Asn Ala Ala Ala Ala Pro Ala
130 135 140Glu Glu Glu Ala Ala Pro Ala Glu Glu Glu Glu Pro Val Lys Glu Glu145 150 155 160Pro Lys Lys Glu Glu Pro Lys Lys Glu Glu Pro Lys Lys Glu Ala Ala
165 170 175Thr Thr Pro Ala Ala Ala Ser Ala Thr Val Ser Ala Ser Gly Asp Asn
180 185 190Val Pro Tyr Val Thr Pro Leu Val Arg Lys Leu Ala Glu Lys His Gly
195 200 205Val Asp Leu Asn Thr Val Thr Gly Thr Gly Ile Gly Gly Arg Ile Arg
210 215 220Lys Gln Asp Val Leu Ala Ala Ala Asn Gly Glu Ala Ala Pro Ala Glu225 230 235 240Ala Ala Ala Pro Val Ser Ala Trp Ser Thr Lys Ser Val Asp Pro Glu
245 250 255Lys Ala Lys Leu Arg Gly Thr Thr Gln Lys Val Asn Arg Ile Arg Glu
260 265 270Ile Thr Ala Met Lys Thr Val Glu Ala Leu Gln Ile Ser Ala Gln Leu
275 280 285Thr Gln Leu His Glu Val Asp Met Thr Arg Val Ala Glu Leu Arg Lys
290 295 300Lys Asn Lys Pro Ala Phe Ile Glu Lys His Gly Val Asn Leu Thr Tyr305 310 315 320Leu Pro Phe Phe Val Lys Ala Val Val Glu Ala Leu Val Ser His Pro
325 330 335Asn Val Asn Ala Ser Phe Asn Ala Lys Thr Lys Glu Met Thr Tyr His
340 345 350Ser Ser Val Asn Leu Ser Ile Ala Val Asp Thr Pro Ala Gly Leu Leu
355 360 365Thr Pro Val Ile His Asp Ala Gln Asp Leu Ser Ile Pro Glu Ile Ala
370 375 380Lys Ala Ile Val Asp Leu Ala Asp Arg Ser Arg Asn Asn Lys Leu Lys385 390 395 400Pro Asn Asp Leu Ser Gly Gly Thr Phe Thr Ile Thr Asn Ile Gly Ser
405 410 415Glu Gly Ala Leu Ser Asp Thr Pro Ile Leu Val Pro Pro Gln Ala Gly
420 425 430Ile Leu Gly Thr Gly Ala Ile Val Lys Arg Pro Val Val Ile Thr Glu
435 440 445Asp Gly Ile Asp Ser Ile Ala Ile Arg Gln Met Val Phe Leu Pro Leu
450 455 460Thr Tyr Asp His Gln Val Val Asp Gly Ala Asp Ala Gly Arg Phe Leu465 470 475 480Thr Thr Ile Lys Asp Arg Leu Glu Thr Ala Asn Phe Glu Gly Asp Leu
485 490 495Gln Leu<210>565<211>1005<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(982)<223>RXA00782<400>565gggtatggat gcagcggctg atcacgctgc ccatttggcc aatcttgccc agcacggcca 60gttcgcaacc gctaattagt taaggagcac ctgtttaatc atg tct att ttt ctc 115
Met Ser Ile Phe Leu
1 5aat tca gat tcc cgc atc atc att cag ggc att acc ggt tcg gaa ggt 163Asn Ser Asp Ser Arg Ile Ile Ile Gln Gly Ile Thr Gly Ser Glu Gly
10 15 20tca gag cat gcg cgt cga att tta gcc tct ggt gcg aag ctc gtg ggt 211Ser Glu His Ala Arg Arg Ile Leu Ala Ser Gly Ala Lys Leu Val Gly
25 30 35ggc acc aac ccc cgc aaa gct ggg caa acc att ttg atc aat gac act 259Gly Thr Asn Pro Arg Lys Ala Gly Gln Thr Ile Leu Ile Asn Asp Thr
40 45 50gag ttg cct gta ttt ggc act gtt aag gaa gca atg gag gaa acg ggt 307Glu Leu Pro Val Phe Gly Thr Val Lys Glu Ala Met Glu Glu Thr Gly
55 60 65gcg gat gtc acc gta att ttc gtt cct cca gcc ttt gcc aaa gct gcg 355Ala Asp Val Thr Val Ile Phe Val Pro Pro Ala Phe Ala Lys Ala Ala70 75 80 85atc att gaa gct atc gac gct cac atc cca ctg tgc gtg att att act 403Ile Ile Glu Ala Ile Asp Ala His Ile Pro Leu Cys Val Ile Ile Thr
90 95 100gag ggc atc cca gtg cgt gac gct tct gag gcg tgg gct tat gcc aag 451Glu Gly Ile Pro Val Arg Asp Ala Ser Glu Ala Trp Ala Tyr Ala Lys
105 110 115aag gtg gga cac acc cgc atc att ggc cct aac tgc cca ggc att att 499Lys Val Gly His Thr Arg Ile Ile Gly Pro Asn Cys Pro Gly Ile Ile
120 125 130act ccc ggc gaa tct ctt gcg gga att acg ccg gca aac att gca ggt 547Thr Pro Gly Glu Ser Leu Ala Gly Ile Thr Pro Ala Asn Ile Ala Gly
135 140 145tcc ggc ccg atc ggg ttg atc tca aag tcg gga aca ctg act tat cag 595Ser Gly Pro Ile Gly Leu Ile Ser Lys Ser Gly Thr Leu Thr Tyr Gln150 155 160 165atg atg tac gaa ctt tca gat att ggc att tct acg gcg att ggt att 643Met Met Tyr Glu Leu Ser Asp Ile Gly Ile Ser Thr Ala Ile Gly Ile
170 175 180ggc ggt gac cca atc atc ggt aca acc cat atc gac gct ctg gag gcc 691Gly Gly Asp Pro Ile Ile Gly Thr Thr His Ile Asp Ala Leu Glu Ala
185 190 195ttt gaa gct gat cct gag acc aag gca atc gtc atg atc ggt gag atc 739Phe Glu Ala Asp Pro Glu Thr Lys Ala Ile Val Met Ile Gly Glu Ile
200 205 210ggt gga gat gca gag gaa cgc gct gct gac ttc att tct aag cac gtg 787Gly Gly Asp Ala Glu Glu Arg Ala Ala Asp Phe Ile Ser Lys His Val
215 220 225aca aaa cca gtt gtg ggt tac gtg gca ggc ttt acc gcc cct gaa gga 835Thr Lys Pro Val Val Gly Tyr Val Ala Gly Phe Thr Ala Pro Glu Gly230 235 240 245aag acc atg ggg cat gct ggc gcc atc gtg aca ggt tca gaa ggc act 883Lys Thr Met Gly His Ala Gly Ala Ile Val Thr Gly Ser Glu Gly Thr
250 255 260gcg cga gca aag aag cat gca ttg gag gcc gtg ggt gtt cgc gtg gga 931Ala Arg Ala Lys Lys His Ala Leu Glu Ala Val Gly Val Arg Val Gly
265 270 275aca act ccg agt gaa acc gcg aag ctt atg cgt gag gta gtt gca gct 979Thr Thr Pro Ser Glu Thr Ala Lys Leu Met Arg Glu Val Val Ala Ala
280 285 290ttg taactaacag gccacagatc tta 1005Leu<210>566<211>294<212>PRT<213>谷氨酸棒杆菌<400>566Met Ser Ile Phe Leu Asn Ser Asp Ser Arg Ile Ile Ile Gln Gly Ile1 5 10 15Thr Gly Ser Glu Gly Ser Glu His Ala Arg Arg Ile Leu Ala Ser Gly
20 25 30Ala Lys Leu Val Gly Gly Thr Asn Pro Arg Lys Ala Gly Gln Thr Ile
35 40 45Leu Ile Asn Asp Thr Glu Leu Pro Val Phe Gly Thr Val Lys Glu Ala
50 55 60Met Glu Glu Thr Gly Ala Asp Val Thr Val Ile Phe Val Pro Pro Ala65 70 75 80Phe Ala Lys Ala Ala Ile Ile Glu Ala Ile Asp Ala His Ile Pro Leu
85 90 95Cys Val Ile Ile Thr Glu Gly Ile Pro Val Arg Asp Ala Ser Glu Ala
100 105 110Trp Ala Tyr Ala Lys Lys Val Gly His Thr Arg Ile Ile Gly Pro Asn
115 120 125Cys Pro Gly Ile Ile Thr Pro Gly Glu Ser Leu Ala Gly Ile Thr Pro
130 135 140Ala Asn Ile Ala Gly Ser Gly Pro Ile Gly Leu Ile Ser Lys Ser Gly145 150 155 160Thr Leu Thr Tyr Gln Met Met Tyr Glu Leu Ser Asp Ile Gly Ile Ser
165 170 175Thr Ala Ile Gly Ile Gly Gly Asp Pro Ile Ile Gly Thr Thr His Ile
180 185 190Asp Ala Leu Glu Ala Phe Glu Ala Asp Pro Glu Thr Lys Ala Ile Val
195 200 205Met Ile Gly Glu Ile Gly Gly Asp Ala Glu Glu Arg Ala Ala Asp Phe
210 215 220Ile Ser Lys His Val Thr Lys Pro Val Val Gly Tyr Val Ala Gly Phe225 230 235 240Thr Ala Pro Glu Gly Lys Thr Met Gly His Ala Gly Ala Ile Val Thr
245 250 255Gly Ser Glu Gly Gly Thr Ala Arg Ala Lys Lys His Ala Leu Glu Ala Val
260 265 270Gly Val Arg Val Gly Thr Thr Pro Ser Glu Thr Ala Lys Leu Met Arg
275 280 285Glu Val Val Ala Ala Leu
290<210>567<211>1395<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1372)<223>RXA00783<400>567aaagttccca aggggtgggg gctgagcacc acggatccaa ttttgttgca atttgcaaag 60tttacagtgt tagacttcac aatacgatca tattggtgag ttg aaa cac tta ctt 115
Leu Lys His Leu Leu
1 5tta cgg gaa gac ttt gtt aaa gac gca gaa ggc tct aag cat ggg ccg 163Leu Arg Glu Asp Phe Val Lys Asp Ala Glu Gly Ser Lys His Gly Pro
10 15 20gaa atg gaa ttg gca gtg gat ctt ttt gaa tac caa gca cgg gac ctc 211Glu Met Glu Leu Ala Val Asp Leu Phe Glu Tyr Gln Ala Arg Asp Leu
25 30 35ttt gaa acc cat ggt gtg cca gtg ttg aag gga att gtg gca tca aca 259Phe Glu Thr His Gly Val Pro Val Leu Lys Gly Ile Val Ala Ser Thr
40 45 50cca gag gcg gcg agg aaa gcg gct gag gaa atc ggc gga ctg acc gtc 307Pro Glu Ala Ala Arg Lys Ala Ala Glu Glu Ile Gly Gly Leu Thr Val
55 60 65gtc aag gct cag gtc aag gtg ggc gga cgt ggc aag gcg ggt ggc gtc 355Val Lys Ala Gln Val Lys Val Gly Gly Arg Gly Lys Ala Gly Gly Val70 75 80 85cgt gtg gca ccg acg tcg gct cag gct ttt gat gct gcg gat gcg att 403Arg Val Ala Pro Thr Ser Ala Gln Ala Phe Asp Ala Ala Asp Ala Ile
90 95 100ctc ggc atg gat atc aaa gga cac act gtt aat cag gtg atg gtg gcg 451Leu Gly Met Asp Ile Lys Gly His Thr Val Asn Gln Val Met Val Ala
105 110 115cag ggc gct gac att gct gag gaa tac tat ttc tcc att ttg ttg gat 499Gln Gly Ala Asp Ile Ala Glu Glu Tyr Tyr Phe Ser Ile Leu Leu Asp
120 125 130cgc gcg aat cgt tcg tat ctg gct atg tgc tct gtt gaa ggt ggc atg 547Arg Ala Asn Arg Ser Tyr Leu Ala Met Cys Ser Val Glu Gly Gly Met
135 140 145gag atc gag atc ctg gcg aag gaa aag cct gaa gct ttg gca aag gtg 595Glu Ile Glu Ile Leu Ala Lys Glu Lys Pro Glu Ala Leu Ala Lys Val150 155 160 165gaa gtg gat ccc ctc act ggt att gat gag gac aaa gcg cgg gag att 643Glu Val Asp Pro Leu Thr Gly Ile Asp Glu Asp Lys Ala Arg Glu Ile
170 175 180gtc act gct gct ggc ttt gaa act gag gtg gca gag aaa gtc att ccg 691Val Thr Ala Ala Gly Phe Glu Thr Glu Val Ala Glu Lys Val Ile Pro
185 190 195gtg ctg atc aag atc tgg cag gtg tat tac gaa gag gaa gca aca ctc 739Val Leu Ile Lys Ile Trp Gln Val Tyr Tyr Glu Glu Glu Ala Thr Leu
200 205 210gtt gag gtg aac ccg ttg gtg ctc acg gat gac ggc gat gtg att gcg 787Val Glu Val Asn Pro Leu Val Leu Thr Asp Asp Gly Asp Val Ile Ala
215 220 225ctt gat ggc aag atc acg ctg gat gat aac gct gat ttc cgc cat gat 835Leu Asp Gly Lys Ile Thr Leu Asp Asp Asn Ala Asp Phe Arg His Asp230 235 240 245aac cgt ggt gcg ttg gct gaa tct gcc ggt ggc ttg gac att ttg gaa 883Asn Arg Gly Ala Leu Ala Glu Ser Ala Gly Gly Leu Asp Ile Leu Glu
250 255 260ctg aag gcc aag aag aat gat ctg aac tac gtg aaa ctt gat ggc tct 931Leu Lys Ala Lys Lys Asn Asp Leu Asn Tyr Val Lys Leu Asp Gly Ser
265 270 275gtg ggc atc att ggc aat ggt gca ggt ttg gtg atg tcc acg ttg gat 979Val Gly Ile Ile Gly Asn Gly Ala Gly Leu Val Met Ser Thr Leu Asp
280 285 290atc gtg gct gca gct ggt gaa cgc cat ggt ggg cag cgc ccc gcg aac 1027Ile Val Ala Ala Ala Gly Glu Arg His Gly Gly Gln Arg Pro Ala Asn
295 300 305ttc cta gac att ggt ggc gga gca tca gct gaa tcg atg gct gct ggt 1075Phe Leu Asp Ile Gly Gly Gly Ala Ser Ala Glu Ser Met Ala Ala Gly310 315 320 325ctc gat gtg atc ctt ggg gat agc cag gta cgc agt gtg ttt gtg aat 1123Leu Asp Val Ile Leu Gly Asp Ser Gln Val Arg Ser Val Phe Val Asn
330 335 340gtg ttt ggt ggc atc acc gcg tgt gat gtg gtg gca aag gga atc gtt 1171Val Phe Gly Gly Ile Thr Asa Cys Asp Val Val Ala Lys Gly Ile Val
345 350 355gga gct ttg gat gtg ctc ggc gat caa gca acg aag cct ctt gtg gtg 1219Gly Ala Leu Asp Val Leu Gly Asp Gln Ala Thr Lys Pro Leu Val Val
360 365 370cgc ctt gat ggc aac aac gtg gtg gaa ggc aga cga atc ctc gcg gaa 1267Arg Leu Asp Gly Asn Asn Val Val Glu Gly Arg Arg Ile Leu Ala Glu
375 380 385tat aac cac cct ttg gtc acc gtt gtg gag ggt atg gat gca gcg gct 1315Tyr Asn His Pro Leu Val Thr Val Val Glu Gly Met Asp Ala Ala Ala390 395 400 405gat cac gct gcc cat ttg gcc aat ctt gcc cag cac ggc cag ttc gca 1363Asp His Ala Ala His Leu Ala Asn Leu Ala Gln His Gly Gln Phe Ala
410 415 420acc gct aat tagttaagga gcacctgttt aat 1395Thr Ala Asn<210>568<211>424<212>PRT<213>谷氨酸棒杆菌<400>568Leu Lys His Leu Leu Leu Arg Glu Asp Phe Val Lys Asp Ala Glu Gly1 5 10 15Ser Lys His Gly Pro Glu Met Glu Leu Ala Val Asp Leu Phe Glu Tyr
20 25 30Gln Ala Arg Asp Leu Phe Glu Thr His Gly Val Pro Val Leu Lys Gly
35 40 45Ile Val Ala Ser Thr Pro Glu Ala Ala Arg Lys Ala Ala Glu Glu Ile
50 55 60Gly Gly Leu Thr Val Val Lys Ala Gln Val Lys Val Gly Gly Arg Gly65 70 75 80Lys Ala Gly Gly Val Arg Val Ala Pro Thr Ser Ala Gln Ala Phe Asp
85 90 95Ala Ala Asp Ala Ile Leu Gly Met Asp Ile Lys Gly His Thr Val Asn
100 105 110Gln Val Met Val Ala Gln Gly Ala Asp Ile Ala Glu Glu Tyr Tyr Phe
115 120 125Ser Ile Leu Leu Asp Arg Ala Asn Arg Ser Tyr Leu Ala Met Cys Ser
130 135 140Val Glu Gly Gly Met Glu Ile Glu Ile Leu Ala Lys Glu Lys Pro Glu145 150 155 160Ala Leu Ala Lys Val Glu Val Asp Pro Leu Thr Gly Ile Asp Glu Asp
165 170 175Lys Ala Arg Glu Ile Val Thr Ala Ala Gly Phe Glu Thr Glu Val Ala
180 185 190Glu Lys Val Ile Pro Val Leu Ile Lys Ile Trp Gln Val Tyr Tyr Glu
195 200 205Glu Glu Ala Thr Leu Val Glu Val Asn Pro Leu Val Leu Thr Asp Asp
210 215 220Gly Asp Val Ile Ala Leu Asp Gly Lys Ile Thr Leu Asp Asp Asn Ala225 230 235 240Asp Phe Arg His Asp Asn Arg Gly Ala Leu Ala Glu Ser Ala Gly Gly
245 250 255Leu Asp Ile Leu Glu Leu Lys Ala Lys Lys Asn Asp Leu Asn Tyr Val
260 265 270Lys Leu Asp Gly Ser Val Gly Ile Ile Gly Asn Gly Ala Gly Leu Val
275 280 285Met Ser Thr Leu Asp Ile Val Ala Ala Ala Gly Glu Arg His Gly Gly
290 295 300Gln Arg Pro Ala Asn Phe Leu Asp Ile Gly Gly Gly Ala Ser Ala Glu305 310 315 320Ser Met Ala Ala Gly Leu Asp Val Ile Leu Gly Asp Ser Gln Val Arg
325 330 335Ser Val Phe Val Asn Val Phe Gly Gly Ile Thr Ala Cys Asp Val Val
340 345 350Ala Lys Gly Ile Val Gly Ala Leu Asp Val Leu Gly Asp Gln Ala Thr
355 360 365Lys Pro Leu Val Val Arg Leu Asp Gly Asn Asn Val Val Glu Gly Arg
370 375 380Arg Ile Leu Ala Glu Tyr Asn His Pro Leu Val Thr Val Val Glu Gly385 390 395 400Met Asp Ala Ala Ala Asp His Ala Ala His Leu Ala Asn Leu Ala Gln
405 410 415His Gly Gln Phe Ala Thr Ala Asn
420<210>569<211>1623<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1600)<223>RXN01695<400>569tatggccaac acttgcattc gggtgctggc gatcatttat gagatgacgc cttgtgttgg 60tgttcggcag agaactcgcg gagataaaag gaagttgaac atg tca gat tcc ccg 115
Met Ser Asp Ser Pro
1 5aag aac gca ccg agg att acc gat gag gca gat gta gtt ctc att ggt 163Lys Asn Ala Pro Arg Ile Thr Asp Glu Ala Asp Val Val Leu Ile Gly
10 15 20gcc ggt atc atg agc tcc acg ctg ggt gca atg ctg cgt cag ctg gag 211Ala Gly Ile Met Ser Ser Thr Leu Gly Ala Met Leu Arg Gln Leu Glu
25 30 35cca agc tgg act cag atc gtc ttc gag cgt ttg gat gga ccg gca caa 259Pro Ser Trp Thr Gln Ile Val Phe Glu Arg Leu Asp Gly Pro Ala Gln
40 45 50gag tcg tcc tcc ccg tgg aac aat gca gga acc ggc cac tct gct cta 307Glu Ser Ser Ser Pro Trp Asn Asn Ala Gly Thr Gly His Ser Ala Leu
55 60 65tgc gag ctg aac tac acc cca gag gtt aag ggc aag gtt gaa att gcc 355Cys Glu Leu Asn Tyr Thr Pro Glu Val Lys Gly Lys Val Glu Ile Ala70 75 80 85aag gct gta gga atc aac gag aag ttc cag gtt tcc cgt cag ttc tgg 403Lys Ala Val Gly Ile Asn Glu Lys Phe Gln Val Ser Arg Gln Phe Trp
90 95 100tct cac ctc gtt gaa gag gga gtg ctg tct gat cct aag gaa ttc atc 451Ser His Leu Val Glu Glu Gly Val Leu Ser Asp Pro Lys Glu Phe Ile
105 110 115aac cct gtt cct cac gta tct ttc ggc cag ggc gca gat cag gtt gca 499Asn Pro Val Pro His Val Ser Phe Gly Gln Gly Ala Asp Gln Val Ala
120 125 130tac atc aag gct cgc tac gaa gct ttg aag gat cac cca ctc ttc cag 547Tyr Ile Lys Ala Arg Tyr Glu Ala Leu Lys Asp His Pro Leu Phe Gln
135 140 145ggc atg acc tac gct gac gat gaa gct acc ttc acc gag aag ctg cct 595Gly Met Thr Tyr Ala Asp Asp Glu Ala Thr Phe Thr Glu Lys Leu Pro150 155 160 165ttg atg gca aag ggc cgt gac ttc tct gat cca gta gca atc tct tgg 643Leu Met Ala Lys Gly Arg Asp Phe Ser Asp Pro Val Ala Ile Ser Trp
170 175 180atc gat gaa ggc acc gac atc aac tac ggt gct cag acc aag cag tac 691Ile Asp Glu Gly Thr Asp Ile Asn Tyr Gly Ala Gln Thr Lys Gln Tyr
185 190 195ctg gat gca gct gaa gtt gaa ggc act gaa atc cgc tat ggc cac gaa 739Leu Asp Ala Ala Glu Val Glu Gly Thr Glu Ile Arg Tyr Gly His Glu
200 205 210gtc aag agc atc aag gct gat ggc gca aag tgg atc gtg acc gtc aag 787Val Lys Ser Ile Lys Ala Asp Gly Ala Lys Trp Ile Val Thr Val Lys
215 220 225aac gta cac act ggc gac acc aag acc atc aag gca aac ttc gtg ttc 835Asn Val His Thr Gly Asp Thr Lys Thr Ile Lys Ala Asn Phe Val Phe230 235 240 245gtc ggc gca ggc gga tac gca ctg gat ctg ctt cgc agc gca ggc atc 883Val Gly Ala Gly Gly Tyr Ala Leu Asp Leu Leu Arg Ser Ala Gly Ile
250 255 260cca cag gtc aag ggc ttc gct gga ttc cca gta tcc ggc ctg tgg ctt 931Pro Gln Val Lys Gly Phe Ala Gly Phe Pro Val Ser Gly Leu Trp Leu
265 270 275cgt tgc acc aac gag gaa ctg atc gag cag cac gca gcc aag gta tat 979Arg Cys Thr Asn Glu Glu Leu Ile Glu Gln His Ala Ala Lys Val Tyr
280 285 290ggc aag gca tct gtt ggc gct cct cca atg tct gtt cct cac ctt gac 1027Gly Lys Ala Ser Val Gly Ala Pro Pro Met Ser Val Pro His Leu Asp
295 300 305acc cgc gtt atc gag ggt gaa aag ggt ctg ctc ttt gga cct tac ggt 1075Thr Arg Val Ile Glu Gly Glu Lys Gly Leu Leu Phe Gly Pro Tyr Gly310 315 320 325ggc tgg acc cct aag ttc ttg aag gaa ggc tcc tac ctg gac ctg ttc 1123Gly Trp Thr Pro Lys Phe Leu Lys Glu Gly Ser Tyr Leu Asp Leu Phe
330 335 340aag tcc atc cgc cca gac aac att cct tcc tac ctt ggc gtt gct gct 1171Lys Ser Ile Arg Pro Asp Asn Ile Pro Ser Tyr Leu Gly Val Ala Ala
345 350 355cag gaa ttt gat ctg acc aag tac ctt gtc act gaa gtt ctc aag gac 1219Gln Glu Phe Asp Leu Thr Lys Tyr Leu ValThr Glu Val Leu Lys Asp
360 365 370cag gac aag cgt atg gat gct ctt cgc gag tac atg cca gag gca caa 1267Gln Asp Lys Arg Met Asp Ala Leu Arg Glu Tyr Met Pro Glu Ala Gln
375 380 385aac ggc gat tgg gag acc atc gtt gcc gga cag cgt gtt cag gtt att 1315Asn Gly Asp Trp Glu Thr Ile Val Ala Gly Gln Arg Val Gln Val Ile390 395 400 405aag cct gca gga ttc cct aag ttc ggt tcc ctg gaa ttc ggc acc acc 1363Lys Pro Ala Gly Phe Pro Lys Phe Gly Ser Leu Glu Phe Gly Thr Thr
410 415 420ttg atc aac aac tcc gaa ggc acc atc gcc gga ttg ctc ggt gct tcc 1411Leu Ile Asn Asn Ser Glu Gly Thr Ile Ala Gly Leu Leu Gly Ala Ser
425 430 435cct gga gca tcc atc gca cct tcc gca atg atc gag ctg ctt gag cgt 1459Pro Gly Ala Ser Ile Ala Pro Ser Ala Met Ile Glu Leu Leu Glu Arg
440 445 450tgc ttc ggt gac cgc atg atc gag tgg ggc gac aag ctg aag gac atg 1507Cys Phe Gly Asp Arg Met Ile Glu Trp Gly Asp Lys Leu Lys Asp Met
455 460 465atc cct tcc tac ggc aag aag ctt gct tcc gag cca gca ctg ttt gag 1555Ile Pro Ser Tyr Gly Lys Lys Leu Ala Ser Glu Pro Ala Leu Phe Glu470 475 480 485cag cag tgg gca cgc acc cag aag acc ctg aag ctt gag gaa gcc 1600Gln Gln Trp Ala Arg Thr Gln Lys Thr Leu Lys Leu Glu Glu Ala
490 495 500taaatcttct aactgctttc ttt 1623<210>570<211>500<212>PRT<213>谷氨酸棒杆菌<400>570Met Ser Asp Ser Pro Lys Asn Ala Pro Arg Ile Thr Asp Glu Ala Asp1 5 10 15Val Val Leu Ile Gly Ala Gly Ile Met Ser Ser Thr Leu Gly Ala Met
20 25 30Leu Arg Gln Leu Glu Pro Ser Trp Thr Gln Ile Val Phe Glu Arg Leu
35 40 45Asp Gly Pro Ala Gln Glu Ser Ser Ser Pro Trp Asn Asn Ala Gly Thr
50 55 60Gly His Ser Ala Leu Cys Glu Leu Asn Tyr Thr Pro Glu Val Lys Gly65 70 75 80Lys Val Glu Ile Ala Lys Ala Val Gly Ile Asn Glu Lys Phe Gln Val
85 90 95Ser Arg Gln Phe Trp Ser His Leu Val Glu Glu Gly Val Leu Ser Asp
100 105 110Pro Lys Glu Phe Ile Asn Pro Val Pro His Val Ser Phe Gly Gln Gly
115 120 125Ala Asp Gln Val Ala Tyr Ile Lys Ala Arg Tyr Glu Ala Leu Lys Asp
130 135 140His Pro Leu Phe Gln Gly Met Thr Tyr Ala Asp Asp Glu Ala Thr Phe145 150 155 160Thr Glu Lys Leu Pro Leu Met Ala Lys Gly Arg Asp Phe Ser Asp Pro
165 170 175Val Ala Ile Ser Trp Ile Asp Glu Gly Thr Asp Ile Asn Tyr Gly Ala
180 185 190Gln Thr Lys Gln Tyr Leu Asp Ala Ala Glu Val Glu Gly Thr Glu Ile
195 200 205Arg Tyr Gly His Glu Val Lys Ser Ile Lys Ala Asp Gly Ala Lys Trp
210 215 220Ile Val Thr Val Lys Asn Val His Thr Gly Asp Thr Lys Thr Ile Lys225 230 235 240Ala Asn Phe Val Phe Val Gly Ala Gly Gly Tyr Ala Leu Asp Leu Leu
245 250 255Arg Ser Ala Gly Ile Pro Gln Val Lys Gly Phe Ala Gly Phe Pro Val
260 265 270Ser Gly Leu Trp Leu Arg Cys Thr Asn Glu Glu Leu Ile Glu Gln His
275 280 285Ala Ala Lys Val Tyr Gly Lys Ala Ser Val Gly Ala Pro Pro Met Ser
290 295 300Val Pro His Leu Asp Thr Arg Val Ile Glu Gly Glu Lys Gly Leu Leu305 310 315 320Phe Gly Pro Tyr Gly Gly Trp Thr Pro Lys Phe Leu Lys Glu Gly Ser
325 330 335Tyr Lau Asp Leu Phe Lys Ser Ile Pro Asp Asn Ile Pro Ser Tyr
340 345 350Leu Gly Val Ala Ala Gln Glu Phe Asp Leu Thr Lys Tyr Leu Val Thr
355 360 365Glu Val Leu Lys Asp Gln Asp Lys Arg Met Asp Ala Leu Arg Glu Tyr
370 375 380Met Pro Glu Ala Gln Asn Gly Asp Trp Glu Thr Ile Val Ala Gly Gln385 390 395 400Arg Val Gln Val Ile Lys Pro Ala Gly Phe Pro Lys Phe Gly Ser Leu
405 410 415Glu Phe Gly Thr Thr Leu Ile Asn Asn Ser Glu Gly Thr Ile Ala Gly
420 425 430Leu Leu Gly Ala Ser Pro Gly Ala Ser Ile Ala Pro Ser Ala Met Ile
435 440 445Glu Leu Leu Glu Arg Cys Phe Gly Asp Arg Met Ile Glu Trp Gly Asp
450 455 460Lys Leu Lys Asp Met Ile Pro Ser Tyr Gly Lys Lys Leu Ala Ser Glu465 470 475 480Pro Ala Leu Phe Glu Gln Gln Trp Ala Arg Thr Gln Lys Thr Leu Lys
485 490 495Leu Glu Glu Ala
500<210>571<211>1039<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1039)<223>FRXA01615<400>571tatggccaac acttgcattc gggtgctggc gatcatttat gagatgacgc cttgtgttgg 60tgttcggcag agaactcgcg gagataaaag gaagttgaac atg tca gat tcc ccg 115
Met Ser Asp Ser Pro
1 5aag aac gca ccg agg att acc gat gag gca gat gta gtt ctc att ggt 163Lys Asn Ala Pro Arg Ile Thr Asp Glu Ala Asp Val Val Leu Ile Gly
10 15 20gcc ggt atc atg agc tcc acg ctg ggt gca atg ctg cgt cag ctg gag 211Ala Gly Ile Met Ser Ser Thr Leu Gly Ala Met Leu Arg Gln Leu Glu
25 30 35cca agc tgg act cag atc gtc ttc gag cgt ttg gat gga ccg gca caa 259Pro Ser Trp Thr Gln Ile Val Phe Glu Arg Leu Asp Gly Pro Ala Gln
40 45 50gag tcg tcc tcc ccg tgg aac aat gca gga acc ggc cac tct gct cta 307Glu Ser Ser Ser Pro Trp Asn Asn Ala Gly Thr Gly His Ser Ala Leu
55 60 65tgc gag ctg aac tac acc cca gag gtt aag ggc aag gtt gaa att gcc 355Cys Glu Leu Asn Tyr Thr Pro Glu Val Lys Gly Lys Val Glu Ile Ala70 75 80 85aag gct gta gga atc aac gag aag ttc cag gtt tcc cgt cag ttc tgg 403Lys Ala Val Gly Ile Asn Glu Lys Phe Gln Val Ser Arg Gln Phe Trp
90 95 100tct cac ctc gtt gaa gag gga gtg ctg tct gat cct aag gaa ttc atc 451Ser His Leu Val Glu Glu Gly Val Leu Ser Asp Pro Lys Glu Phe Ile
105 110 115aac cct gtt cct cac gta tct ttc ggc cag ggc gca gat cag gtt gca 499Asn Pro Val Pro His Val Ser Phe Gly Gln Gly Ala Asp Gln Val Ala
120 125 130tac atc aag gct cgc tac gaa gct ttg aag gat cac cca ctc ttc cag 547Tyr Ile Lys Ala Arg Tyr Glu Ala Leu Lys Asp His Pro Leu Phe Gln
135 140 145ggc atg acc tac gct gac gat gaa gct acc ttc acc gag aag ctg cct 595Gly Met Thr Tyr Ala Asp Asp Glu Ala Thr Phe Thr Glu Lys Leu Pro150 155 160 165ttg atg gca aag ggc cgt gac ttc tct gat cca gta gca atc tct tgg 643Leu Met Ala Lys Gly Arg Asp Phe Ser Asp Pro Val Ala Ile Ser Trp
170 175 180atc gat gaa ggc acc gac atc aac tac ggt gct cag acc aag cag tac 691Ile Asp Glu Gly Thr Asp Ile Asn Tyr Gly Ala Gln Thr Lys Gln Tyr
185 190 195ctg gat gca gct gaa gtt gaa ggc act gaa atc cgc tat ggc cac gaa 739Leu Asp Ala Ala Glu Val Glu Gly Thr Glu Ile Arg Tyr Gly His Glu
200 205 210gtc aag agc atc aag gct gat ggc gca aag tgg atc gtg acc gtc aag 787Val Lys Ser Ile Lys Ala Asp Gly Ala Lys Trp Ile Val Thr Val Lys
215 220 225aac gta cac act ggc gac acc aag acc atc aag gca aac ttc gtg ttc 835Asn Val His Thr Gly Asp Thr Lys Thr Ile Lys Ala Asn Phe Val Phe230 235 240 245gtc ggc gca ggc gga tac gca ctg gat ctg ctt cgc agc gca ggc atc 883Val Gly Ala Gly Gly Tyr Ala Leu Asp Leu Leu Arg Ser Ala Gly Ile
250 255 260cca cag gtc aag ggc ttc gct gga ttc cca gta tcc ggc ctg tgg ctt 931Pro Gln Val Lys Gly Phe Ala Gly Phe Pro Val Ser Gly Leu Trp Leu
265 270 275cgt tgc acc aac gag gaa ctg atc gag cag cac gca gcc aag gta tat 979Arg Cys Thr Asn Glu Glu Leu Ile Glu Gln His Ala Ala Lys Val Tyr
280 285 290ggc aag gca tct gtt ggc gct cct cca atg tct gtt cct cac ctt gac 1027Gly Lys Ala Ser Val Gly Ala Pro Pro Met Ser Val Pro His Leu Asp
295 300 305acc cgc gtt atc 1039Thr Arg Val Ile310<210>572<211>313<212>PRT<213>谷氨酸棒杆菌<400>572Met Ser Asp Ser Pro Lys Asn Ala Pro Arg Ile Thr Asp Glu Ala Asp1 5 10 15Val Val Leu Ile Gly Ala Gly Ile Met Ser Ser Thr Leu Gly Ala Met
20 25 30Leu Arg Gln Leu Glu Pro Ser Trp Thr Gln Ile Val Phe Glu Arg Leu
35 40 45Asp Gly Pro Ala Gln Glu Ser Ser Ser Pro Trp Asn Asn Ala Gly Thr
50 55 60Gly His Ser Ala Leu Cys Glu Leu Asn Tyr Thr Pro Glu Val Lys Gly65 70 75 80Lys Val Glu Ile Ala Lys Ala Val Gly Ile Asn Glu Lys Phe Gln Val
85 90 95Ser Arg Gln Phe Trp Ser His Leu Val Glu Glu Gly Val Leu Ser Asp
100 105 110Pro Lys Glu Phe Ile Asn Pro Val Pro His Val Ser Phe Gly Gln Gly
115 120 125Ala Asp Gln Val Ala Tyr Ile Lys Ala Arg Tyr Glu Ala Leu Lys Asp
130 135 140His Pro Leu Phe Gln Gly Met Thr Tyr Ala Asp Asp Glu Ala Thr Phe145 150 155 160Thr Glu Lys Leu Pro Leu Met Ala Lys Gly Arg Asp Phe Ser Asp Pro
165 170 175Val Ala Ile Ser Trp Ile Asp Glu Gly Thr Asp Ile Asn Tyr Gly Ala
180 185 190Gln Thr Lys Gln Tyr Leu Asp Ala Ala Glu Val Glu Gly Thr Glu Ile
195 200 205Arg Tyr Gly His Glu Val Lys Ser Ile Lys Ala Asp Gly Ala Lys Trp
210 215 220Ile Val Thr Val Lys Asn Val His Thr Gly Asp Thr Lys Thr Ile Lys225 230 235 240Ala Asn Phe Val Phe Val Gly Ala Gly Gly Tyr Ala Leu Asp Leu Leu
245 250 255Arg Ser Ala Gly Ile Pro Gln Val Lys Gly Phe Ala Gly Phe Pro Val
260 265 270Ser Gly Leu Trp Leu Arg Cys Thr Asn Glu Glu Leu Ile Glu Gln His
275 280 285Ala Ala Lys Val Tyr Gly Lys Ala Ser Val Gly Ala Pro Pro Met Ser
290 295 300Val Pro His Leu Asp Thr Arg Val Ile305 310<210>573<211>233<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(210)<223>FRXA01695<400>573gcc gga ttg ctc ggt gct tcc cct gga gca tcc atc gca cct tcc gca 48Ala Gly Leu Leu Gly Ala Ser Pro Gly Ala Ser Ile Ala Pro Ser Ala1 5 10 15atg atc gag ctg ctt gag cgt tgc ttc ggt gac cgc atg atc gag tgg 96Met Ile Glu Leu Leu Glu Arg Cys Phe Gly Asp Arg Met Ile Glu Trp
20 25 30ggc gac aag ctg aag gac atg atc cct tcc tac ggc aag aag ctt gct 144Gly Asp Lys Leu Lys Asp Met Ile Pro Ser Tyr Gly Lys Lys Leu Ala
35 40 45tcc gag cca gca ctg ttt gag cag cag tgg gca cgc acc cag aag acc 192Ser Glu Pro Ala Leu Phe Glu Gln Gln Trp Ala Arg Thr Gln Lys Thr
50 55 60ctg aag ctt gag gaa gcc taaatcttct aactgctttc ttt 233Leu Lys Leu Glu Glu Ala65 70<210>574<211>70<212>PRT<213>谷氨酸棒杆菌<400>574Ala Gly Leu Leu Gly Ala Ser Pro Gly Ala Ser Ile Ala Pro Ser Ala1 5 10 15Met Ile Glu Leu Leu Glu Arg Cys Phe Gly Asp Arg Met Ile Glu Trp
20 25 30Gly Asp Lys Leu Lys Asp Met Ile Pro Ser Tyr Gly Lys Lys Leu Ala
35 40 45Ser Glu Pro Ala Leu Phe Glu Gln Gln Trp Ala Arg Thr Gln Lys Thr
50 55 60Leu Lys Leu Glu Glu Ala65 70<210>575<211>1063<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1063)<223>RXA00290<400>575agctacagat ttagctagtg tttttgttcc agaaccctaa atgaggttct acccttaaca 60gagcttcccg caaaaacacc gattaacaag gctaaatgat atg acc atc gac ctg 115
Met Thr Ile Asp Leu
1 5cag cgt tcc acc caa aac ctc acc cat gag gaa atc ttc gag gca cac 163Gln Arg Ser Thr Gln Asn Leu Thr His Glu Glu Ile Phe Glu Ala His
10 15 20gag ggc gga aag ctc tcc att agt tcc act cgt ccg ctc cgc gac atg 211Glu Gly Gly Lys Leu Ser Ile Ser Ser Thr Arg Pro Leu Arg Asp Met
25 30 35cgc gat ctt tcc ctt gct tac acc cct ggt gtt gct cag gtt tgt gaa 259Arg Asp Leu Ser Leu Ala Tyr Thr Pro Gly Val Ala Gln Val Cys Glu
40 45 50gca atc aag gaa gat cca gag gtt gcg cgc acc cac acg ggc att gga 307Ala Ile Lys Glu Asp Pro Glu Val Ala Arg Thr His Thr Gly Ile Gly
55 60 65aac acc gtc gcg gtt att tcc gac ggc acc gct gtt ctt ggc ctt ggc 355Asn Thr Val Ala Val Ile Ser Asp Gly Thr Ala Val Leu Gly Leu Gly70 75 80 85gat atc gga cct cag gcc tcc ctt ccc gtc atg gag ggc aag gct cag 403Asp Ile Gly Pro Gln Ala Ser Leu Pro Val Met Glu Gly Lys Ala Gln
90 95 100ctg ttt agc tct ttc gct ggc ctg aag gct atc cct atc gtt ttg gac 451Leu Phe Ser Ser Phe Ala Gly Leu Lys Ala Ile Pro Ile Val Leu Asp
105 110 115gtt cac gat gtt gac gct ttg gtt gag acc atc gca gcc atc gcg cct 499Val His Asp Val Asp Ala Leu Val Glu Thr Ile Ala Ala Ile Ala Pro
120 125 130tct ttc ggt gct atc aac ttg gag gac atc tcc gct cct cgt tgc ttc 547Ser Phe Gly Ala Ile Asn Leu Glu Asp Ile Ser Ala Pro Arg Cys Phe
135 140 145gag gtg gag cgc cgc ctc atc gag cgt ctc gat att cca gtt atg cac 595Glu Val Glu Arg Arg Leu Ile Glu Arg Leu Asp Ile Pro Val Met His150 155 160 165gat gac cag cac ggc acc gct gtg gtt atc ctc gct gcg ctg cgc aac 643Asp Asp Gln His Gly Thr Ala Val Val Ile Leu Ala Ala Leu Arg Asn
170 175 180tcc ctg aag ctg ctg gat cgc aag atc gaa gac ctc aag att gtt att 691Ser Leu Lys Leu Leu Asp Arg Lys Ile Glu Asp Leu Lys Ile Val Ile
185 190 195tcc ggc gca ggc gca gcg ggc gtt gca gct gta gat atg ctg acc aac 739Ser Gly Ala Gly Ala Ala Gly Val Ala Ala Val Asp Met Leu Thr Asn
200 205 210gct gga gca acc gac atc gtg gtt ctt gat tcc cga ggc atc atc cac 787Ala Gly Ala Thr Asp Ile Val Val Leu Asp Ser Arg Gly Ile Ile His
215 220 225gac agc cgt gag gat ctt tcc cca gtt aag gct gct ctt gca gag aag 835Asp Ser Arg Glu Asp Leu Ser Pro Val Lys Ala Ala Leu Ala Glu Lys230 235 240 245acc aac cct cgt ggc atc agc ggt ggc atc aat gag gct ttc acc ggc 883Thr Asn Pro Arg Gly Ile Ser Gly Gly Ile Asn Glu Ala Phe Thr Gly
250 255 260gcg gac ctg ttc att ggc gtg tcc ggc ggc aac atc ggc gag gac gct 931Ala Asp Leu Phe Ile Gly Val Ser Gly Gly Asn Ile Gly Glu Asp Ala
265 270 275ctc aaa ctc atg gcc ccg gag cca atc ctg ttc acc ctg gcg aac cca 979Leu Lys Leu Met Ala Pro Glu Pro Ile Leu Phe Thr Leu Ala Asn Pro
280 285 290acc cca gag atc gat cct gag ctg tct cag aag tac ggc gcc atc gtc 1027Thr Pro Glu Ile Asp Pro Glu Leu Ser Gln Lys Tyr Gly Ala Ile Val
295 300 305gcg acc ggg ccg gtc ttg acc tgc cta acc aga tca 1063Ala Thr Gly Pro Val Leu Thr Cys Leu Thr Arg Ser310 315 320<210>576<211>321<212>PRT<213>谷氨酸棒杆菌<400> 576Met Thr Ile Asp Leu Gln Arg Ser Thr Gln Asn Leu Thr His Glu Glu1 5 10 15Ile Phe Glu Ala His Glu Gly Gly Lys Leu Ser Ile Ser Ser Thr Arg
20 25 30Pro Leu Arg Asp Met Arg Asp Leu Ser Leu Ala Tyr Thr Pro Gly Val
35 40 45Ala Gln Val Cys Glu Ala Ile Lys Glu Asp Pro Glu Val Ala Arg Thr
50 55 60His Thr Gly Ile Gly Asn Thr Val Ala Val Ile Ser Asp Gly Thr Ala65 70 75 80Val Leu Gly Leu Gly Asp Ile Gly Pro Gln Ala Ser Leu Pro Val Met
85 90 95Glu Gly Lys Ala Gln Leu Phe Ser Ser Phe Ala Gly Leu Lys Ala Ile
100 105 110Pro Ile Val Leu Asp Val His Asp Val Asp Ala Leu Val Glu Thr Ile
115 120 125Ala Ala Ile Ala Pro Ser Phe Gly Ala Ile Asn Leu Glu Asp Ile Ser
130 135 140Ala Pro Arg Cys Phe Glu Val Glu Arg Arg Leu Ile Glu Arg Leu Asp145 150 155 160Ile Pro Val Met His Asp Asp Gln His Gly Thr Ala Val Val Ile Leu
165 170 175Ala Ala Leu Arg Asn Ser Leu Lys Leu Leu Asp Arg Lys Ile Glu Asp
180 185 190Leu Lys Ile Val Ile Ser Gly Ala Gly Ala Ala Gly Val Ala Ala Val
195 200 205Asp Met Leu Thr Asn Ala Gly Ala Thr Asp Ile Val Val Leu Asp Ser
210 215 220Arg Gly Ile Ile His Asp Ser Arg Glu Asp Leu Ser Pro Val Lys Ala225 230 235 240Ala Leu Ala Glu Lys Thr Asn Pro Arg Gly Ile Ser Gly Gly Ile Asn
245 250 255Glu Ala Phe Thr Gly Ala Asp Leu Phe Ile Gly Val Ser Gly Gly Asn
260 265 270Ile Gly Glu Asp Ala Leu Lys Leu Met Ala Pro Glu Pro Ile Leu Phe
275 280 285Thr Leu Ala Asn Pro Thr Pro Glu Ile Asp Pro Glu Leu Ser Gln Lys
290 295 300Tyr Gly Ala Ile Val Ala Thr Gly Pro Val Leu Thr Cys Leu Thr Arg305 310 315 320Ser<210>577<211>1347<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1324)<223>RXN01048<400>577agctacagat ttagctagtg tttttgttcc agaaccctaa atgaggttct acccttaaca 60gagcttcccg caaaaacacc gattaacaag gctaaatgat atg acc atc gac ctg 115
Met Thr Ile Asp Leu
1 5cag cgt tcc acc caa aac ctc acc cat gag gaa atc ttc gag gca cac 163Gln Arg Ser Thr Gln Asn Leu Thr His Glu Glu Ile Phe Glu Ala His
10 15 20gag ggc gga aag ctc tcc att agt tcc act cgt ccg ctc cgc gac atg 211Glu Gly Gly Lys Leu Ser Ile Ser Ser Thr Arg Pro Leu Arg Asp Met
25 30 35cgc gat ctt tcc ctt gct tac acc cct ggt gtt gct cag gtt tgt gaa 259Arg Asp Leu Ser Leu Ala Tyr Thr Pro Gly Val Ala Gln Val Cys Glu
40 45 50gca atc aag gaa gat cca gag gtt gcg cgc acc cac acg ggc att gga 307Ala Ile Lys Glu Asp Pro Glu Val Ala Arg Thr His Thr Gly Ile Gly
55 60 65aac acc gtc gcg gtt att tcc gac ggc acc gct gtt ctt ggc ctt ggc 355Asn Thr Val Ala Val Ile Ser Asp Gly Thr Ala Val Leu Gly Leu Gly70 75 80 85gat atc gga cct cag gcc tcc ctt ccc gtc atg gag ggc aag gct cag 403Asp Ile Gly Pro Gln Ala Ser Leu Pro Val Met Glu Gly Lys Ala Gln
90 95 100ctg ttt agc tct ttc gct ggc ctg aag gct atc cct atc gtt ttg gac 451Leu Phe Ser Ser Phe Ala Gly Leu Lys Ala Ile Pro Ile Val Leu Asp
105 110 115gtt cac gat gtt gac gct ttg gtt gag acc atc gca gcc atc gcg cct 499Val His Asp Val Asp Ala Leu Val Glu Thr Ile Ala Ala Ile Ala Pro
120 125 130tct ttc ggt gct atc aac ttg gag gac atc tcc gct cct cgt tgc ttc 547Ser Phe Gly Ala Ile Asn Leu Glu Asp Ile Ser Ala Pro Arg Cys Phe
135 140 145gag gtg gag cgc cgc ctc atc gag cgt ctc gat att cca gtt atg cac 595Glu Val Glu Arg Arg Leu Ile Glu Arg Leu Asp Ile Pro Val Met His150 155 160 165gat gac cag cac ggc acc gct gtg gtt atc ctc gct gcg ctg cgc aac 643Asp Asp Gln His Gly Thr Ala Val Val Ile Leu Ala Ala Leu Arg Asn
170 175 180tcc ctg aag ctg ctg gat cgc aag atc gaa gac ctc aag att gtt att 691Ser Leu Lys Leu Leu Asp Arg Lys Ile Glu Asp Leu Lys Ile Val Ile
185 190 195tcc ggc gca ggc gca gcg ggc gtt gca gct gta gat atg ctg acc aac 739Ser Gly Ala Gly Ala Ala Gly Val Ala Ala Val Asp Met Leu Thr Asn
200 205 210gct gga gca acc gac atc gtg gtt ctt gat tcc cga ggc atc atc cac 787Ala Gly Ala Thr Asp Ile Val Val Leu Asp Ser Arg Gly Ile Ile His
215 220 225gac agc cgt gag gat ctt tcc cca gtt aag gct gct ctt gca gag aag 835Asp Ser Arg Glu Asp Leu Ser Pro Val Lys Ala Ala Leu Ala Glu Lys230 235 240 245acc aac cct cgt ggc atc agc ggt ggc atc aat gag gct ttc acc ggc 883Thr Asn Pro Arg Gly Ile Ser Gly Gly Ile Asn Glu Ala Phe Thr Gly
250 255 260gcg gac ctg ttc att ggc gtg tcc ggc ggc aac atc ggc gag gac gct 931Ala Asp Leu Phe Ile Gly Val Ser Gly Gly Asn Ile Gly Glu Asp Ala
265 270 275ctc aaa ctc atg gcc ccg gag cca atc ctg ttc acc ctg gcg aac cca 979Leu Lys Leu Met Ala Pro Glu Pro Ile Leu Phe Thr Leu Ala Asn Pro
280 285 290acc cca gag atc gat cct gag ctg tct cag aag tac ggc gcc atc gtc 1027Thr Pro Glu Ile Asp Pro Glu Leu Ser Gln Lys Tyr Gly Ala Ile Val
295 300 305gcg acc ggg cgc tct gac ctg cct aac cag atc aac aac gtg ctg gcg 1075Ala Thr Gly Arg Ser Asp Leu Pro Asn Gln Ile Asn Asn Val Leu Ala310 315 320 325ttc cca gga att ttc gcc ggc gct ctc gca gcc aag gct aag aag atc 1123Phe Pro Gly Ile Phe Ala Gly Ala Leu Ala Ala Lys Ala Lys Lys Ile
330 335 340acc ccc gag atg aag ctc gcc gct cag agg caa tcg cag aca tcg cag 1171Thr Pro Glu Met Lys Leu Ala Ala Gln Arg Gln Ser Gln Thr Ser Gln
345 350 355ctg agg acc tcg agg tcg gcc gca tcg tgc cta ccg ccc tgg atc ccc 1219Leu Arg Thr Ser Arg Ser Ala Ala Ser Cys Leu Pro Pro Trp Ile Pro
360 365 370gcg ttg ccc caa cag tca agg cag ctg tcc agg ccg tcg cca aag cgc 1267Ala Leu Pro Gln Gln Ser Arg Gln Leu Ser Arg Pro Ser Pro Lys Arg
375 380 385aaa acg ctt aaa aat ttg ctt atc gac gcc tcc ctc ccc gtc gag gcg 1315Lys Thr Leu Lys Asn Leu Leu Ile Asp Ala Ser Leu Pro Val Glu Ala390 395 400 405cca ata ttt taagagcaaa cttgaggccc aca 1347Pro Ile Phe<210>578<211>408<212>PRT<213>谷氨酸棒杆菌<400>578Met Thr Ile Asp Leu Gln Arg Ser Thr Gln Asn Leu Thr His Glu Glu1 5 10 15Ile Phe Glu Ala His Glu Gly Gly Lys Leu Ser Ile Ser Ser Thr Arg
20 25 30Pro Leu Arg Asp Met Arg Asp Leu Ser Leu Ala Tyr Thr Pro Gly Val
35 40 45Ala Gln Val Cys Glu Ala Ile Lys Glu Asp Pro Glu Val Ala Arg Thr
50 55 60His Thr Gly Ile Gly Asn Thr Val Ala Val Ile Ser Asp Gly Thr Ala65 70 75 80Val Leu Gly Leu Gly Asp Ile Gly Pro Gln Ala Ser Leu Pro Val Met
85 90 95Glu Gly Lys Ala Gln Leu Phe Ser Ser Phe Ala Gly Leu Lys Ala Ile
100 105 110Pro Ile Val Leu Asp Val His Asp Val Asp Ala Leu Val Glu Thr Ile
115 120 125Ala Ala Ile Ala Pro Ser Phe Gly Ala Ile Asn Leu Glu Asp Ile Ser
130 135 140Ala Pro Arg Cys Phe Glu Val Glu Arg Arg Leu Ile Glu Arg Leu Asp145 150 155 160Ile Pro Val Met His Asp Asp Gln His Gly Thr Ala Val Val Ile Leu
165 170 175Ala Ala Leu Arg Asn Ser Leu Lys Leu Leu Asp Arg Lys Ile Glu Asp
180 185 190Leu Lys Ile Val Ile Ser Gly Ala Gly Ala Ala Gly Val Ala Ala Val
195 200 205Asp Met Leu Thr Asn Ala Gly Ala Thr Asp Ile Val Val Leu Asp Ser
210 215 220Arg Gly Ile Ile His Asp Ser Arg Glu Asp Leu Ser Pro Val Lys Ala225 230 235 240Ala Leu Ala Glu Lys Thr Asn Pro Arg Gly Ile Ser Gly Gly Ile Asn
245 250 255Glu Ala Phe Thr Gly Ala Asp Leu Phe Ile Gly Val Ser Gly Gly Asn
260 265 270Ile Gly Glu Asp Ala Leu Lys Leu Met Ala Pro Glu Pro Ile Leu Phe
275 280 285Thr Leu Ala Asn Pro Thr Pro Glu Ile Asp Pro Glu Leu Ser Gln Lys
290 295 300Tyr Gly Ala Ile Val Ala Thr Gly Arg Ser Asp Leu Pro Asn Gln Ile305 310 315 320Asn Asn Val Leu Ala Phe Pro Gly Ile Phe Ala Gly Ala Leu Ala Ala
325 330 335Lys Ala Lys Lys Ile Thr Pro Glu Met Lys Leu Ala Ala Gln Arg Gln
340 345 350Ser Gln Thr Ser Gln Leu Arg Thr Ser Arg Ser Ala Ala Ser Cys Leu
355 360 365Pro Pro Trp Ile Pro Ala Leu Pro Gln Gln Ser Arg Gln Leu Ser Arg
370 375 380Pro Ser Pro Lys Arg Lys Thr Leu Lys Asn Leu Leu Ile Asp Ala Ser385 390 395 400Leu Pro Val Glu Ala Pro Ile Phe
405<210>579<211>311<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(288)<223>FRXA01048<400>579cgc tct gac ttg cct aac cag atc aac aac gtg ctg gcg ttc cca gga 48Arg Ser Asp Leu Pro Asn Gln Ile Asn Asn Val Leu Ala Phe Pro Gly1 5 10 15att ttc gcc ggc gct ctc gca gcc aag gct aag aag atc acc ccc gag 96Ile Phe Ala Gly Ala Leu Ala Ala Lys Ala Lys Lys Ile Thr Pro Glu
20 25 30atg aag ctc gcc gct cag agg caa tcg cag aca tcg cag ctg agg acc 144Met Lys Leu Ala Ala Gln Arg Gln Ser Gln Thr Ser Gln Leu Arg Thr
35 40 45tcg agg tcg gcc gca tcg tgc cta ccg ccc tgg atc ccc gcg ttg ccc 192Ser Arg Ser Ala Ala Ser Cys Leu Pro Pro Trp Ile Pro Ala Leu Pro
50 55 60caa cag tca agg cag ctg tcc agg ccg tcg cca aag cgc aaa acg ctt 240Gln Gln Ser Arg Gln Leu Ser Arg Pro Ser Pro Lys Arg Lys Thr Leu65 70 75 80aaa aat ttg ctt atc gac gcc tcc ctc ccc gtc gag gcg cca ata ttt 288Lys Asn Leu Leu Ile Asp Ala Ser Leu Pro Val Glu Ala Pro Ile Phe
85 90 95taagagcaaa cttgaggccc aca 311<210>580<211>96<212>PRT<213>谷氨酸棒杆菌<400>580Arg Ser Asp Leu Pro Asn Gln Ile Asn Asn Val Leu Ala Phe Pro Gly1 5 10 15Ile Phe Ala Gly Ala Leu Ala Ala Lys Ala Lys Lys Ile Thr Pro Glu
20 25 30Met Lys Leu Ala Ala Gln Arg Gln Ser Gln Thr Ser Gln Leu Arg Thr
35 40 45Ser Arg Ser Ala Ala Ser Cys Leu Pro Pro Trp Ile Pro Ala Leu Pro
50 55 60Gln Gln Ser Arg Gln Leu Ser Arg Pro Ser Pro Lys Arg Lys Thr Leu65 70 75 80Lys Asn Leu Leu Ile Asp Ala Ser Leu Pro Val Glu Ala Pro Ile Phe
85 90 95<210>581<211>1063<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1063)<223>FRXA00290<400>581agctacagat ttagctagtg tttttgttcc agaaccctaa atgaggttct acccttaaca 60gagcttcccg caaaaacacc gattaacaag gctaaatgat atg acc atc gac ctg 115
Met Thr Ile Asp Leu
1 5cag cgt tcc acc caa aac ctc acc cat gag gaa atc ttc gag gca cac 163Gln Arg Ser Thr Gln Asn Leu Thr His Glu Glu Ile Phe Glu Ala His
10 15 20gag ggc gga aag ctc tcc att agt tcc act cgt ccg ctc cgc gac atg 211Glu Gly Gly Lys Leu Ser Ile Ser Ser Thr Arg Pro Leu Arg Asp Met
25 30 35cgc gat ctt tcc ctt gct tac acc cct ggt gtt gct cag gtt tgt gaa 259Arg Asp Leu Ser Leu Ala Tyr Thr Pro Gly Val Ala Gln Val Cys Glu
40 45 50gca atc aag gaa gat cca gag gtt gcg cgc acc cac acg ggc att gga 307Ala Ile Lys Glu Asp Pro Glu Val Ala Arg Thr His Thr Gly Ile Gly
55 60 65aac acc gtc gcg gtt att tcc gac ggc acc gct gtt ctt ggc ctt ggc 355Asn Thr Val Ala Val Ile Ser Asp Gly Thr Ala Val Leu Gly Leu Gly70 75 80 85gat atc gga cct cag gcc tcc ctt ccc gtc atg gag ggc aag gct cag 403Asp Ile Gly Pro Gln Ala Ser Leu Pro Val Met Glu Gly Lys Ala Gln
90 95 100ctg ttt agc tct ttc gct ggc ctg aag gct atc cct atc gtt ttg gac 451Leu Phe Ser Ser Phe Ala Gly Leu Lys Ala Ile Pro Ile Val Leu Asp
105 110 115gtt cac gat gtt gac gct ttg gtt gag acc atc gca gcc atc gcg cct 499Val His Asp Val Asp Ala Leu Val Glu Thr Ile Ala Ala Ile Ala Pro
120 125 130tct ttc ggt gct atc aac ttg gag gac atc tcc gct cct cgt tgc ttc 547Ser Phe Gly Ala Ile Asn Leu Glu Asp Ile Ser Ala Pro Arg Cys Phe
135 140 145gag gtg gag cgc cgc ctc atc gag cgt ctc gat att cca gtt atg cac 595Glu Val Glu Arg Arg Leu Ile Glu Arg Leu Asp Ile Pro Val Met His150 155 160 165gat gac cag cac ggc acc gct gtg gtt atc ctc gct gcg ctg cgc aac 643Asp Asp Gln His Gly Thr Ala Val Val Ile Leu Ala Ala Leu Arg Asn
170 175 180tcc ctg aag ctg ctg gat cgc aag atc gaa gac ctc aag att gtt att 691Ser Leu Lys Leu Leu Asp Arg Lys Ile Glu Asp Leu Lys Ile Val Ile
185 190 195tcc ggc gca ggc gca gcg ggc gtt gca gct gta gat atg ctg acc aac 739Ser Gly Ala Gly Ala Ala Gly Val Ala Ala Val Asp Met Leu Thr Asn
200 205 210gct gga gca acc gac atc gtg gtt ctt gat tcc cga ggc atc atc cac 787Ala Gly Ala Thr Asp Ile Val Val Leu Asp Ser Arg Gly Ile Ile His
215 220 225gac agc cgt gag gat ctt tcc cca gtt aag gct gct ctt gca gag aag 835Asp Ser Arg Glu Asp Leu Ser Pro Val Lys Ala Ala Leu Ala Glu Lys230 235 240 245acc aac cct cgt ggc atc agc ggt ggc atc aat gag gct ttc acc ggc 883Thr Asn Pro Arg Gly Ile Ser Gly Gly Ile Asn Glu Ala Phe Thr Gly
250 255 260gcg gac ctg ttc att ggc gtg tcc ggc ggc aac atc ggc gag gac gct 931Ala Asp Leu Phe Ile Gly Val Ser Gly Gly Asn Ile Gly Glu Asp Ala
265 270 275ctc aaa ctc atg gcc ccg gag cca atc ctg ttc acc ctg gcg aac cca 979Leu Lys Leu Met Ala Pro Glu Pro Ile Leu Phe Thr Leu Ala Asn Pro
280 285 290acc cca gag atc gat cct gag ctg tct cag aag tac ggc gcc atc gtc 1027Thr Pro Glu Ile Asp Pro Glu Leu Ser Gln Lys Tyr Gly Ala Ile Val
295 300 305gcg acc ggg ccg gtc ttg acc tgc cta acc aga tca 1063Ala Thr Gly Pro Val Leu Thr Cys Leu Thr Arg Ser310 315 320<210>582<211>321<212>PRT<213>谷氨酸棒杆菌<400>582Met Thr Ile Asp Leu Gln Arg Ser Thr Gln Asn Leu Thr His Glu Glu1 5 10 15Ile Phe Glu Ala His Glu Gly Gly Lys Leu Ser Ile Ser Ser Thr Arg
20 25 30Pro Leu Arg Asp Met Arg Asp Leu Ser Leu Ala Tyr Thr Pro Gly Val
35 40 45Ala Gln Val Cys Glu Ala Ile Lys Glu Asp Pro Glu Val Ala Arg Thr
50 55 60His Thr Gly Ile Gly Asn Thr Val Ala Val Ile Ser Asp Gly Thr Ala65 70 75 80Val Leu Gly Leu Gly Asp Ile Gly Pro Gln Ala Ser Leu Pro Val Met
85 90 95Glu Gly Lys Ala Gln Leu Phe Ser Ser Phe Ala Gly Leu Lys Ala Ile
100 105 110Pro Ile Val Leu Asp Val His Asp Val Asp Ala Leu Val Glu Thr Ile
115 120 125Ala Ala Ile Ala Pro Ser Phe Gly Ala Ile Asn Leu Glu Asp Ile Ser
130 135 140Ala Pro Arg Cys Phe Glu Val Glu Arg Arg Leu Ile Glu Arg Leu Asp145 150 155 160Ile Pro Val Met His Asp Asp Gln His Gly Thr Ala Val Val Ile Leu
165 170 175Ala Ala Leu Arg Asn Ser Leu Lys Leu Leu Asp Arg Lys Ile Glu Asp
180 185 190Leu Lys Ile Val Ile Ser Gly Ala Gly Ala Ala Gly Val Ala Ala Val
195 200 205Asp Met Leu Thr Asn Ala Gly Ala Thr Asp Ile Val Val Leu Asp Ser
210 215 220Arg Gly Ile Ile His Asp Ser Arg Glu Asp Leu Ser Pro Val Lys Ala225 230 235 240Ala Leu Ala Glu Lys Thr Asn Pro Arg Gly Ile Ser Gly Gly Ile Asn
245 250 255Glu Ala Phe Thr Gly Ala Asp Leu Phe Ile Gly Val Ser Gly Gly Asn
260 265 270Ile Gly Glu Asp Ala Leu Lys Leu Met Ala Pro Glu Pro Ile Leu Phe
275 280 285Thr Leu Ala Asn Pro Thr Pro Glu Ile Asp Pro Glu Leu Ser Gln Lys
290 295 300Tyr Gly Ala Ile Val Ala Thr Gly Pro Val Leu Thr Cys Leu Thr Arg305 310 315 320Ser<210>583<211>582<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(582)<223>RXN03101<400>583atc ctt gca gac gaa gac gac acc gtc gac gtc ggc gca gtc atc gcc 48Ile Leu Ala Asp Glu Asp Asp Thr Val Asp Val Gly Ala Val Ile Ala1 5 10 15cgc atc ggt gac gca aac gca gct gca gca cct gcc gaa gag gaa gca 96Arg Ile Gly Asp Ala Asn Ala Ala Ala Ala Pro Ala Glu Glu Glu Ala
20 25 30gct cct gcc gaa gag gaa gaa cca gtt aag gaa gag cca aag aag gag 144Ala Pro Ala Glu Glu Glu Glu Pro Val Lys Glu Glu Pro Lys Lys Glu
35 40 45gca gct cct gaa gct cca gca gca act ggc gcc gca acc gat gtg gaa 192Ala Ala Pro Glu Ala Pro Ala Ala Thr Gly Ala Ala Thr Asp Val Glu
50 55 60atg cca gaa ctc ggc gaa tcc gtc acc gaa ggc acc att acc cag tgg 240Met Pro Glu Glu Gly Glu Ser Val Thr Glu Gly Thr Ile Thr Gln Trp65 70 75 80ctc aag gct gtc ggc gac acc gtc gaa gta gac gaa cca ctt ctt gag 288Leu Lys Ala Val Gly Asp Thr Val Glu Val Asp Glu Pro Leu Leu Glu
85 90 95gtc tcc acc gac aag gtc gac acc gaa atc cca tcc cca gta gca ggc 336Val Ser Thr Asp Lys Val Asp Thr Glu Ile Pro Ser Pro Val Ala Gly
100 105 110acc atc gtg gag atc ctt gca gac gaa gac gac acc gtc gac gtc ggc 384Thr Ile Val Glu Ile Leu Ala Asp Glu Asp Asp Thr Val Asp Val Gly
115 120 125gca gtc atc gcc cgc atc ggt gac gca aac gca gct gca gca cct gcc 432Ala Val Ile Ala Arg Ile Gly Asp Ala Asn Ala Ala Ala Ala Pro Ala
130 135 140gaa gag gaa gca gct cct gcc gaa gag gag gaa cca gtt aag gaa gag 480Glu Glu Glu Ala Ala Pro Ala Glu Glu Glu Glu Pro Val Lys Glu Glu145 150 155 160cca aag aag gaa gag ccc aag aag gaa gag ccc aag aag gaa gca gct 528Pro Lys Lys Glu Glu Pro Lys Lys Glu Glu Pro Lys Lys Glu Ala Ala
165 170 175act aca cct gct gcg gca tcc gca act gtg tcc gct tct ggc gac aac 576Thr Thr Pro Ala Ala Ala Ser Ala Thr Val Ser Ala Ser Gly Asp Asn
180 185 190gtt cca 582Val Pro<210>584<211>194<212>PRT<213>谷氨酸棒杆菌<400>584Ile Leu Ala Asp Glu Asp Asp Thr Val Asp Val Gly Ala Val Ile Ala1 5 10 15Arg Ile Gly Asp Ala Asn Ala Ala Ala Ala Pro Ala Glu Glu Glu Ala
20 25 30Ala Pro Ala Glu Glu Glu Glu Pro Val Lys Glu Glu Pro Lys Lys Glu
35 40 45Ala Ala Pro Glu Ala Pro Ala Ala Thr Gly Ala Ala Thr Asp Val Glu
50 55 60Met Pro Glu Leu Gly Glu Ser Val Thr Glu Gly Thr Ile Thr Gln Trp65 70 75 80Leu Lys Ala Val Gly Asp Thr Val Glu Val Asp Glu Pro Leu Leu Glu
85 90 95Val Ser Thr Asp Lys Val Asp Thr Glu Ile Pro Ser Pro Val Ala Gly
100 105 110Thr Ile Val Glu Ile Leu Ala Asp Glu Asp Asp Thr Val Asp Val Gly
115 120 125Ala Val Ile Ala Arg Ile Gly Asp Ala Asn Ala Ala Ala Ala Pro Ala
130 135 140Glu Glu Glu Ala Ala Pro Ala Glu Glu Glu Glu Pro Val Lys Glu Glu145 150 155 160Pro Lys Lys Glu Glu Pro Lys Lys Glu Glu Pro Lys Lys Glu Ala Ala
165 170 175Thr Thr Pro Ala Ala Ala Ser Ala Thr Val Ser Ala Ser Gly Asp Asn
180 185 190Val Pro<210>585<211>540<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(517)<223>RXN02046<400>585tccgtgccat caccaccggc gagatcactg gcatcgtgga cgcaaaacaa acagcaacag 60aaattattaa catccgacgc aacgcttcag gagagtcctc atg aaa gag aca ctg 115
Met Lys Glu Thr Leu
1 5acc acc ggt tta acc cac caa atg acc tac ata gtg cca gca aac cgc 163Thr Thr Gly Leu Thr His Gln Met Thr Tyr Ile Val Pro Ala Asn Arg
10 15 20aca gtt ccg cat ctg ctt ccc gaa gca gca gaa ttt gaa acc atg cca 211Thr Val Pro His Leu Leu Pro Glu Ala Ala Glu Phe Glu Thr Met Pro
25 30 35gat gtc ctg gcc act gga tat atg gtc ggc atc atc gag tgg gcc tgc 259Asp Val Leu Ala Thr Gly Tyr Met Val Gly Ile Ile Glu Trp Ala Cys
40 45 50atg gaa ctt ctg cgt ccc cat ttg gac gac ggt gaa atc tcg ctg ggc 307Met Glu Leu Leu Arg Pro His Leu Asp Asp Gly Glu Ile Ser Leu Gly
55 60 65act cat gtg aac ttc tcc cac gca gct cca acg gtt cct gga tcc acg 355Thr His Val Asn Phe Ser His Ala Ala Pro Thr Val Pro Gly Ser Thr70 75 80 85gtc acc atc gat gtt gag gtg aca gag atc aac cgt cgt gca gtt acc 403Val Thr Ile Asp Val Glu Val Thr Glu Ile Asn Arg Arg Ala Val Thr
90 95 100ttc aac atc act gca gct gat gag ttc gcc acc atc agc acc ggc acc 451Phe Asn Ile Thr Ala Ala Asp Glu Phe Ala Thr Ile Ser Thr Gly Thr
105 110 115cac cag cgc ggt gtg gtt aac cgt gag aag ttt gtc tcc cgt ctg cct 499His Gln Arg Gly Val Val Asn Arg Glu Lys Phe Val Ser Arg Leu Pro
120 125 130gaa gca cct aag gaa aac taaatcatgg ccaagttgtt tga 540Glu Ala Pro Lys Glu Asn
135<210>586<211>139<212>PRT<213>谷氨酸棒杆菌<400>586Met Lys Glu Thr Leu Thr Thr Gly Leu Thr His Gln Met Thr Tyr Ile1 5 10 15Val Pro Ala Asn Arg Thr Val Pro His Leu Leu Pro Glu Ala Ala Glu
20 25 30Phe Glu Thr Met Pro Asp Val Leu Ala Thr Gly Tyr Met Val Gly Ile
35 40 45Ile Glu Trp Ala Cys Met Glu Leu Leu Arg Pro His Leu Asp Asp Gly
50 55 60Glu Ile Ser Leu Gly Thr His Val Asn Phe Ser His Ala Ala Pro Thr65 70 75 80Val Pro Gly Ser Thr Val Thr Ile Asp Val Glu Val Thr Glu Ile Asn
85 90 95Arg Arg Ala Val Thr Phe Asn Ile Thr Ala Ala Asp Glu Phe Ala Thr
100 105 110Ile Ser Thr Gly Thr His Gln Arg Gly Val Val Asn Arg Glu Lys Phe
115 120 125Val Ser Arg Leu Pro Glu Ala Pro Lys Glu Asn
130 135<210>587<211>1683<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1660)<223>RXN00389<400>587ccaccactgc gtaacctttc cgagcaagat atcgcggacc tgtcggattt gcttgccacc 60tctggcgcag gttcctaccg ccttcagttg aggtgaaagc atg atc acc gca acc 115
Met Ile Thr Ala Thr
1 5gca ctg cat ggg tgt tca ctg att gat ggc gag tgg gtc gct gga aaa 163Ala Leu His Gly Cys Ser Leu Ile Asp Gly Glu Trp Val Ala Gly Lys
10 15 20aat ggt gag att aca gga ttc gat ccg cgc acc aat gcg agt ctg aac 211Asn Gly Glu Ile Thr Gly Phe Asp Pro Arg Thr Asn Ala Ser Leu Asn
25 30 35cct tcc tac tct tta gca aac agc gca cag ctg cgc gcc gcc aca aca 259Pro Ser Tyr Ser Leu Ala Asn Ser Ala Gln Leu Arg Ala Ala Thr Thr
40 45 50tcg gcg aag cga gct ttt gaa agc tac cga ctc act act cca gag gtt 307Ser Ala Lys Arg Ala Phe Glu Ser Tyr Arg Leu Thr Thr Pro Glu Val
55 60 65aga gca gat ttc ctg gat tcc atc gct gac aac atc gat gcg cta tcc 355Arg Ala Asp Phe Leu Asp Ser Ile Ala Asp Asn Ile Asp Ala Leu Ser70 75 80 85ggc gag atc gtg caa cgg gcg agc ctg gag aca ggt ttg gga act acc 403Gly Glu Ile Val Gln Arg Ala Ser Leu Glu Thr Gly Leu Gly Thr Thr
90 95 100cga ctc aca ggc gaa gta gcc cgc acc agc aac cag ctc cgc ctg ttt 451Arg Leu Thr Gly Glu Val Ala Arg Thr Ser Asn Gln Leu Arg Leu Phe
105 110 115gca gaa acc gtg aga agc gga cag ttc cac cga gta cgc att gaa cga 499Ala Glu Thr Val Arg Ser Gly Gln Phe His Arg Val Arg Ile Glu Arg
120 125 130gga ccg cgg att gat ctt cgc cag cgt cag gtt ccg ttg gga cca gtc 547Gly Pro Arg Ile Asp Leu Arg Gln Arg Gln Val Pro 4eu Gly Pro Val
135 140 145gcg gta ttc ggg gca agc aac ttc ccc gtc gct ttc tct act gct ggt 595Ala Val Phe Gly Ala Ser Asn Phe Pro Val Ala Phe Ser Thr Ala Gly150 155 160 165ggc gat aca gca tca gcg ttg gct gca ggc tgc cct gtg gtt ttt aag 643Gly Asp Thr Ala Ser Ala Leu Ala Ala Gly Cys Pro Val Val Phe Lys
170 175 180gcg cat aat gcg cac cct gga aca gct gag ctc gtc ggg caa gcg gtg 691Ala His Asn Ala His Pro Gly Thr Ala Glu Leu Val Gly Gln Ala Val
185 190 195cgg gga gcc gtc gaa aag cat gag ttt gat gct ggt gtg ttt aac ctt 739Arg Gly Ala Val Glu Lys His Glu Phe Asp Ala Gly Val Phe Asn Leu
200 205 210gtc tac ggc cgt ggc gtg gaa att ggc cag gag ctg gct gcg gat ccg 787Val Tyr Gly Arg Gly Val Glu Ile Gly Gln Glu Leu Ala Ala Asp Pro
215 220 225aat atc acg gca atc ggt ttt acc ggt tca cgc cag ggt ggt ttg gca 835Asn Ile Thr Ala Ile Gly Phe Thr Gly Ser Arg Gln Gly Gly Leu Ala230 235 240 245ctg tca cag act gcg ttt agc cgc cca gtt ccc gtt cca gtc ttt gca 883Leu Ser Gln Thr Ala Phe Ser Arg Pro Val Pro Val Pro Val Phe Ala
250 255 260gaa atg agt gcc acc aac cct gtg ttc gtc ttc ccc ggc gcg ctg gcg 931Glu Met Ser Ala Thr Asn Pro Val Phe Val Phe Pro Gly Ala Leu Ala
265 270 275gat ttg gat gca tcg agt tcc ttg gct gag gcg ttt acc gct tcc gtc 979Asp Leu Asp Ala Ser Ser Ser Leu Ala Glu Ala Phe Thr Ala Ser Val
280 285 290acc ggc agt tcc ggg caa ttg tgc acc aag cct ggc ctc gtt ttc atc 1027Thr Gly Ser Ser Gly Gln Leu Cys Thr Lys Pro Gly Leu Val Phe Ile
295 300 305ccg cgc ggt gtt gtt ggt gat gct ttt gtg gcg ctc gta gca gcc aaa 1075Pro Arg Gly Val Val Gly Asp Ala Phe Val Ala Leu Val Ala Ala Lys310 315 320 325ttt aaa gaa acc acg ggt caa acg atg ctc acg caa ggc atc gct cag 1123Phe Lys Glu Thr Thr Gly Gln Thr Met Leu Thr Gln Gly Ile Ala Gln
330 335 340gca tgg cag cgc gga gtc gac aac ctt gca gca cag cca agt gta aaa 1171Ala Trp Gln Arg Gly Val Asp Asn Leu Ala Ala Gln Pro Ser Val Lys
345 350 355atc ctc gcc caa ggc acc ccc gga gat gga gag aac gcg ccg ggc ccg 1219Ile Leu Ala Gln Gly Thr Pro Gly Asp Gly Glu Asn Ala Pro Gly Pro
360 365 370gtg gtg ttt gaa agt gat gtg cag gcg ttg cta aat aat gtg gtg ttg 1267Val Val Phe Glu Ser Asp Val Gln Ala Leu Leu Asn Asn Val Val Leu
375 380 385cag gaa gaa atc ttc ggt gcg gca tcg ctg gtg gtg cgt tat gat tcc 1315Gln Glu Glu Ile Phe Gly Ala Ala Ser Leu Val Val Arg Tyr Asp Ser390 395 400 405ccg gat caa ctc cac caa gta gcc aat tca ctc gag gga caa tta aca 1363Pro Asp Gln Leu His Gln Val Ala Asn Ser Leu Glu Gly Gln Leu Thr
410 415 420gcc acg atc cac gca tcc cag gat gat ttc cag gaa gtc tcg aaa ctt 1411Ala Thr Ile His Ala Ser Gln Asp Asp Phe Gln Glu Val Ser Lys Leu
425 430 435atc ccc ctc ttg gag gat ctc gcg ggc cgt gtt ctt tac ggc ggc tgg 1459Ile Pro Leu Leu Glu Asp Leu Ala Gly Arg Val Leu Tyr Gly Gly Trp
440 445 450cca acg ggt gtg gaa gtt ggg cac acg gtt atc cat gga ggc cct tat 1507Pro Thr Gly Val Glu Val Gly His Thr Val Ile His Gly Gly Pro Tyr
455 460 465ccg gcg acc tca aat gcg cag tcg aca agt gtt gga acc ctg gca atc 1555Pro Ala Thr Ser Asn Ala Gln Ser Thr Ser Val Gly Thr Leu Ala Ile470 475 480 485gag aga ttt atg cgc ccg gtt tct tat caa act ttc ccg gct gag ctg 1603Glu Arg Phe Met Arg Pro Val Ser Tyr Gln Thr Phe Pro Ala Glu Leu
490 495 500ctt cca gat cca gtt tct gag gcg aat aaa tgg gct gta cct cgg gaa 1651Leu Pro Asp Pro Val Ser Glu Ala Asn Lys Trp Ala Val Pro Arg Glu
505 510 515ata gac cgt taatagctgg tctttacatt tgc 1683Ile Asp Arg
520<210>588<211>520<212>PRT<213>谷氨酸棒杆菌<400>588Met Ile Thr Ala Thr Ala Leu His Gly Cys Ser Leu Ile Asp Gly Glu1 5 10 15Trp Val Ala Gly Lys Asn Gly Glu Ile Thr Gly Phe Asp Pro Arg Thr
20 25 30Asn Ala Ser Leu Asn Pro Ser Tyr Ser Leu Ala Asn Ser Ala Gln Leu
35 40 45Arg Ala Ala Thr Thr Ser Ala Lys Arg Ala Phe Glu Ser Tyr Arg Leu
50 55 60Thr Thr Pro Glu Val Arg Ala Asp Phe Leu Asp Ser Ile Ala Asp Asn65 70 75 80Ile Asp Ala Leu Ser Gly Glu Ile Val Gln Arg Ala Ser Leu Glu Thr
85 90 95Gly Leu Gly Thr Thr Arg Leu Thr Gly Glu Val Ala Arg Thr Ser Asn
100 105 110Gln Leu Arg Leu Phe Ala Glu Thr Val Arg Ser Gly Gln Phe His Arg
115 120 125Val Arg Ile Glu Arg Gly Pro Arg Ile Asp Leu Arg Gln Arg Gln Val
130 135 140Pro Leu Gly Pro Val Ala Val Phe Gly Ala Ser Asn Phe Pro Val Ala145 150 155 160Phe Ser Thr Ala Gly Gly Asp Thr Ala Ser Ala Leu Ala Ala Gly Cys
165 170 175Pro Val Val Phe Lys Ala His Asn Ala His Pro Gly Thr Ala Glu Leu
180 185 190Val Gly Gln Ala Val Arg Gly Ala Val Glu Lys His Glu Phe Asp Ala
195 200 205Gly Val Phe Asn Leu Val Tyr Gly Arg Gly Val Glu Ile Gly Gln Glu
210 215 220Leu Ala Ala Asp Pro Asn Ile Thr Ala Ile Gly Phe Thr Gly Ser Arg225 230 235 240Gln Gly Gly Leu Ala Leu Ser Gln Thr Ala Phe Ser Arg Pro Val Pro
245 250 255Val Pro Val Phe Ala Glu Met Ser Ala Thr Asn Pro Val Phe Val Phe
260 265 270Pro Gly Ala Leu Ala Asp Leu Asp Ala Ser Ser Ser Leu Ala Glu Ala
275 280 285Phe Thr Ala Ser Val Thr Gly Ser Ser Gly Gln Leu Cys Thr Lys Pro
290 295 300Gly Leu Val Phe Ile Pro Arg Gly Val Val Gly Asp Ala Phe Val Ala305 310 315 320Leu Val Ala Ala Lys Phe Lys Glu Thr Thr Gly Gln Thr Met Leu Thr
325 330 335Gln Gly Ile Ala Gln Ala Trp Gln Arg Gly Val Asp Asn Leu Ala Ala
340 345 350Gln Pro Ser Val Lys Ile Leu Ala Gln Gly Thr Pro Gly Asp Gly Glu
355 360 365Asn Ala Pro Gly Pro Val Val Phe Glu Ser Asp Val Gln Ala Leu Leu
370 375 380Asn Asn Val Val Leu Gln Glu Glu Ile Phe Gly Ala Ala Ser Leu Val385 390 395 400Val Arg Tyr Asp Ser Pro Asp Gln Leu His Gln Val Ala Asn Ser Leu
405 410 415Glu Gly Gln Leu Thr Ala Thr Ile His Ala Ser Gln Asp Asp Phe Gln
420 425 430Glu Val Ser Lys Leu Ile Pro Leu Leu Glu Asp Leu Ala Gly Arg Val
435 440 445Leu Tyr Gly Gly Trp Pro Thr Gly Val Glu Val Gly His Thr Val Ile
450 455 460His Gly Gly Pro Tyr Pro Ala Thr Ser Asn Ala Gln Ser Thr Ser Val465 470 475 480Gly Thr Leu Ala Ile Glu Arg Phe Met Arg Pro Val Ser Tyr Gln Thr
485 490 495Phe Pro Ala Glu Leu Leu Pro Asp Pro Val Ser Glu Ala Asn Lys Trp
500 505 510Ala Val Pro Arg Glu Ile Asp Arg
515 520<210>589<211>1467<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1444)<223>RXN02399<400>589ttgacgcacc aatgcccgat ggagcaatgt gtgaaccacg ccaccacgca aaccgatgca 60catcacgtcg aaacagtgac agtgcattag ctcatacttt gtg gtc ggc acc gcc 115
Val Val Gly Thr Ala
1 5cat tgc gaa tca gca ctt aag gaa gtg act ttg atg tca aac gtt gga 163His Cys Glu Ser Ala Leu Lys Glu Val Thr Leu Met Ser Asn Val Gly
10 15 20aag cca cgt acc gca cag gaa atc cag cag gat tgg gac acc aac cct 211Lys Pro Arg Thr Ala Gln Glu Ile Gln Gln Asp Trp Asp Thr Asn Pro
25 30 35cgt tgg aac ggc atc acc cgc gac tac acc gca gac cag gta gct gat 259Arg Trp Asn Gly Ile Thr Arg Asp Tyr Thr Ala Asp Gln Val Ala Asp
40 45 50ctg cag ggt tcc gtc atc gag gag cac act ctt gct cgc cgc ggc tca 307Leu Gln Gly Ser Val Ile Glu Glu His Thr Leu Ala Arg Arg Gly Ser
55 60 65gag atc ctc tgg gac gca gtc acc cag gaa ggt gac gga tac atc aac 355Glu Ile Leu Trp Asp Ala Val Thr Gln Glu Gly Asp Gly Tyr Ile Asn70 75 80 85gcg ctt ggc gca crc acc ggt aac cag gct gtt cag cag gtt cgt gca 403Ala Leu Gly Ala Leu Thr Gly Asn Gln Ala Val Gln Gln Val Arg Ala
90 95 100ggc ctg aag gct gtc tac ctg tcc ggt tgg cag gtc gca ggt gac gcc 451Gly Leu Lys Ala Val Tyr Leu Ser Gly Trp Gln Val Ala Gly Asp Ala
105 110 115aac ctc tcc ggc cac acc tac cct gac cag tcc ctc tac cca gcg aac 499Asn Leu Ser Gly His Thr Tyr Pro Asp Gln Ser Leu Tyr Pro Ala Asn
120 125 130tcc gtt cca agc gtc gtt cgt cgc atc aac aac gca ctg ctg cgt tcc 547Ser Val Pro Ser Val Val Arg Arg Ile Asn Asn Ala Leu Leu Arg Ser
135 140 145gat gaa atc gca cgc acc gaa ggc gac acc tcc gtt gac aac tgg gtt 595Asp Glu Ile Ala Arg Thr Glu Gly Asp Thr Ser Val Asp Asn Trp Val150 155 160 165gtc cca atc gtc gcg gac ggc gaa gct ggc ttc ggt gga gca ctc aac 643Val Pro Ile Val Ala Asp Gly Glu Ala Gly Phe Gly Gly Ala Leu Asn
170 175 180gtc tac gaa ctc cag aag gca atg atc gca gct ggc gct gca ggc acc 691Val Tyr Glu Leu Gln Lys Ala Met Ile Ala Ala Gly Ala Ala Gly Thr
185 190 195cac tgg gaa gac cag ctc gct tct gaa aag aag tgt ggc cac ctc ggc 739His Trp Glu Asp Gln Leu Ala Ser Glu Lys Lys Cys Gly His Leu Gly
200 205 210ggc aag gtt ctg atc cca acc cag cag cac atc cgc acc ctg aac tct 787Gly Lys Val Leu Ile Pro Thr Gln Gln His Ile Arg Thr Leu Asn Ser
215 220 225gcc cgc ctt gca gca gac gtt gca aac acc cca act gtt gtt atc gca 835Ala Arg Leu Ala Ala Asp Val Ala Asn Thr Pro Thr Val Val Ile Ala230 235 240 245cgt acc gac gct gag gca gca acc ctg atc acc tct gac gtt gat gag 883Arg Thr Asp Ala Glu Ala Ala Thr Leu Ile Thr Ser Asp Val Asp Glu
250 255 260cgc gac caa cca ttc atc acc ggt gag cgc acc gca gaa ggc tac tac 931Arg Asp Gln Pro Phe Ile Thr Gly Glu Arg Thr Ala Glu Gly Tyr Tyr
265 270 275cac gtc aag aat ggt ctc gag cca tgt atc gca cgt gca aag tcc tac 979His Val Lys Asn Gly Leu Glu Pro Cys Ile Ala Arg Ala Lys Ser Tyr
280 285 290gca cca tac gca gat atg atc tgg atg gag acc ggc acc cct gac ctg 1027Ala Pro Tyr Ala Asp Met Ile Trp Met Glu Thr Gly Thr Pro Asp Leu
295 300 305gag ctc gct aag aag ttc gct gaa ggc gtt cgc tct gag ttc cca gac 1075Glu Leu Ala Lys Lys Phe Ala Glu Gly Val Arg Ser Glu Phe Pro Asp310 315 320 325cag ctg ctg tcc tac aac tgc tcc cca tcc ttc aac tgg tct gca cac 1123Gln Leu Leu Ser Tyr Asn Cys Ser Pro Ser Phe Asn Trp Ser Ala His
330 335 340ctc gag gca gat gag atc gct aag ttc cag aag gaa ctc ggc gca atg 1171Leu Glu Ala Asp Glu Ile Ala Lys Phe Gln Lys Glu Leu Gly Ala Met
345 350 355ggc ttc aag ttc cag ttc atc acc ctc gca ggc ttc cac tcc ctc aac 1219Gly Phe Lys Phe Gln Phe Ile Thr Leu Ala Gly Phe His Ser Leu Asn
360 365 370tac ggc atg ttc gac ctg gct tac gga tac gct cgc gaa ggc atg acc 1267Tyr Gly Met Phe Asp Leu Ala Tyr Gly Tyr Ala Arg Glu Gly Met Thr
375 380 385tcc ttc gtt gac ctg cag aac cgt gag ttc aag gca gct gaa gag cgt 1315Ser Phe Val Asp Leu Gln Asn Arg Glu Phe Lys Ala Ala Glu Glu Arg390 395 400 405ggc ttc acc gct gtt aag cac cag cgt gag gtt ggc gca ggc tac ttc 1363Gly Phe Thr Ala Val Lys His Gln Arg Glu Val Gly Ala Gly Tyr Phe
410 415 420gac cag atc gca acc acc gtt gac ccg aac tct tct acc acc gct ttg 1411Asp Gln Ile Ala Thr Thr Val Asp Pro Asn Ser Ser Thr Thr Ala Leu
425 430 435aag ggt tcc act gaa gaa ggc cag ttc cac aac taggacctac aggttctgac 1464Lys Gly Ser Thr Glu Glu Gly Gln Phe His Asn
440 445aat 1467<210>590<211>448<212>PRT<213>谷氨酸棒杆菌<400>590Val Val Gly Thr Ala His Cys Glu Ser Ala Leu Lys Glu Val Thr Leu1 5 10 15Met Ser Asn Val Gly Lys Pro Arg Thr Ala Gln Glu Ile Gln Gln Asp
20 25 30Trp Asp Thr Asn Pro Arg Trp Asn Gly Ile Thr Arg Asp Tyr Thr Ala
35 40 45Asp Gln Val Ala Asp Leu Gln Gly Ser Val Ile Glu Glu His Thr Leu
50 55 60Ala Arg Arg Gly Ser Glu Ile Leu Trp Asp Ala Val Thr Gln Glu Gly65 70 75 80Asp Gly Tyr Ile Asn Ala Leu Gly Ala Leu Thr Gly Asn Gln Ala Val
85 90 95Gln Gln Val Arg Ala Gly Leu Lys Ala Val Tyr Leu Ser Gly Trp Gln
100 105 110Val Ala Gly Asp Ala Asn Leu Ser Gly His Thr Tyr Pro Asp Gln Ser
115 120 125Leu Tyr Pro Ala Asn Ser Val Pro Ser Val Val Arg Arg Ile Asn Asn
130 135 140Ala Leu Leu Arg Ser Asp Glu Ile Ala Arg Thr Glu Gly Asp Thr Ser145 150 155 160Val Asp Asn Trp Val Val Pro Ile Val Ala Asp Gly Glu Ala Gly Phe
165 170 175Gly Gly Ala Leu Asn Val Tyr Glu Leu Gln Lys Ala Met Ile Ala Ala
180 185 190Gly Ala Ala Gly Thr His Trp Glu Asp Gln Leu Ala Ser Glu Lys Lys
195 200 205Cys Gly His Leu Gly Gly Lys Val Leu Ile Pro Thr Gln Gln His Ile
210 215 220Arg Thr Leu Asn Ser Ala Arg Leu Ala Ala Asp Val Ala Asn Thr Pro225 230 235 240Thr Val Val Ile Ala Arg Thr Asp Ala Glu Ala Ala Thr Leu Ile Thr
245 250 255Ser Asp Val Asp Glu Arg Asp Gln Pro Phe Ile Thr Gly Glu Arg Thr
260 265 270Ala Glu Gly Tyr Tyr His Val Lys Asn Gly Leu Glu Pro Cys Ile Ala
270 280 285Arg Ala Lys Ser Tyr Ala Pro Tyr Ala Asp Met Ile Trp Met Glu Thr
290 295 300Gly Thr Pro Asp Leu Glu Leu Ala Lys Lys Phe Ala Glu Gly Val Arg305 310 315 320Ser Glu Phe Pro Asp Gln Leu Leu Ser Tyr Asn Cys Ser Pro Ser Phe
325 330 335Asn Trp Ser Ala His Leu Glu Ala Asp Glu Ile Ala Lys Phe Gln Lys
340 345 350Glu Leu Gly Ala Met Gly Phe Lys Phe Gln Phe Ile Thr Leu Ala Gly
355 360 365Phe His Ser Leu Asn Tyr Gly Met Phe Asp Leu Ala Tyr Gly Tyr Ala
370 375 380Arg Glu Gly Met Thr Ser Phe Val Asp Leu Gln Asn Arg Glu Phe Lys385 390 395 400Ala Ala Glu Glu Arg Gly Phe Thr Ala Val Lys His Gln Arg Glu Val
405 410 415Gly Ala Gly Tyr Phe Asp Gln Ile Ala Thr Thr Val Asp Pro Asn Ser
420 425 430Ser Thr Thr Ala Leu Lys Gly Ser Thr Glu Glu Gly Gln Phe His Asn
435 440 445<210>591<211>1419<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1396)<223>FRXA02399<400>591caaaccgatg cacatcacgt cgaaacagtg acagtgcatt agctcatact ttgtggtcgg 60caccgcccat tgcgaatcag cacttaagga agtgactttg atg tca aac gtt gga 115
Met Ser Asn Val Gly
1 5aag cca cgt acc gca cag gaa atc cag cag gat tgg gac acc aac cct 163Lys Pro Arg Thr Ala Gln Glu Ile Gln Gln Asp Trp Asp Thr Asn Pro
10 15 20cgt tgg aac ggc atc acc cgc gac tac acc gca gac cag gta gct gat 211Arg Trp Asn Gly Ile Thr Arg Asp Tyr Thr Ala Asp Gln Val Ala Asp
25 30 35ctg cag ggt tcc gtc atc gag gag cac act ctt gct cgc cgc ggc tca 259Leu Gln Gly Ser Val Ile Glu Glu His Thr Leu Ala Arg Arg Gly Ser
40 45 50gag atc ctc tgg gac gca gtc acc cag gaa ggt gac gga tac atc aac 307Glu Ile Leu Trp Asp Ala Val Thr Gln Glu Gly Asp Gly Tyr Ile Asn
55 60 65gcg ctt ggc gca ctc acc ggt aac cag gct gtt cag cag gtt cgt gca 355Ala Leu Gly Ala Leu Thr Gly Asn Gln Ala Val Gln Gln Val Arg Ala70 75 80 85ggc ctg aag gct gtc tac ctg tcc ggt tgg cag gtc gca ggt gac gcc 403Gly Leu Lys Ala Val Tyr Leu Ser Gly Trp Gln Val Ala Gly Asp Ala
90 95 100aac ctc tcc ggc cac acc tac cct gac cag tcc ctc tac cca gcg aac 451Asn Leu Ser Gly His Thr Tyr Pro Asp Gln Ser Leu Tyr Pro Ala Asn
105 110 115tcc gtt cca agc gtc gtt cgt cgc atc aac aac gca ctg ctg cgt tcc 499Ser Val Pro Ser Val Val Arg Arg Ile Asn Asn Ala Leu Leu Arg Ser
120 125 130gat gaa atc gca cgc acc gaa ggc gac acc tcc gtt gac aac tgg gtt 547Asp Glu Ile Ala Arg Thr Glu Gly Asp Thr Ser Val Asp Asn Trp Val
135 140 145gtc cca atc gtc gcg gac ggc gaa gct ggc ttc ggt gga gca ctc aac 595Val Pro Ile Val Ala Asp Gly Glu Ala Gly Phe Gly Gly Ala Leu Asn150 155 160 165gtc tac gaa ctc cag aag gca atg atc gca gct ggc gct gca ggc acc 643Val Tyr Glu Leu Gln Lys Ala Met Ile Ala Ala Gly Ala Ala Gly Thr
170 175 180cac tgg gaa gac cag ctc gct tct gaa aag aag tgt ggc cac ctc ggc 691His Trp Glu Asp Gln Leu Ala Ser Glu Lys Lys Cys Gly His Leu Gly
185 190 195ggc aag gtt ctg atc cca acc cag cag cac atc cgc acc ctg aac tct 739Gly Lys Val Leu Ile Pro Thr Gln Gln His Ile Arg Thr Leu Asn Ser
200 205 210gcc cgc ctt gca gca gac gtt gca aac acc cca act gtt gtt atc gca 787Ala Arg Leu Ala Ala Asp Val Ala Asn Thr Pro Thr Val Val Ile Ala
215 220 225cgt acc gac gct gag gca gca acc ctg atc acc tct gac gtt gat gag 835Arg Thr Asp Ala Glu Ala Ala Thr Leu Ile Thr Ser Asp Val Asp Glu230 235 240 245cgc gac caa cca ttc atc acc ggt gag cgc acc gca gaa ggc tac tac 883Arg Asp Gln Pro Phe Ile Thr Gly Glu Arg Thr Ala Glu Gly Tyr Tyr
250 255 260cac gtc aag aat ggt ctc gag cca tgt atc gca cgt gca aag tcc tac 931His Val Lys Asn Gly Leu Glu Pro Cys Ile Ala Arg Ala Lys Ser Tyr
265 270 275gca cca tac gca gat atg atc tgg atg gag acc ggc acc cct gac ctg 979Ala Pro Tyr Ala Asp Met Ile Trp Met Glu Thr Gly Thr Pro Asp Leu
280 285 290gag ctc gct aag aag ttc gct gaa ggc gtt cgc tct gag ttc cca gac 1027Glu Leu Ala Lys Lys Phe Ala Glu Gly Val Arg Ser Glu Phe Pro Asp
295 300 305cag ctg ctg tcc tac aac tgc tcc cca tcc ttc aac tgg tct gca cac 1075Gln Leu Leu Ser Tyr Asn Cys Ser Pro Ser Phe Asn Trp Ser Ala His310 315 320 325ctc gag gca gat gag atc gct aag ttc cag aag gaa ctc ggc gca atg 1123Leu Glu Ala Asp Glu Ile Ala Lys Phe Gln Lys Glu Leu Gly Ala Met
330 335 340ggc ttc aag ttc cag ttc atc acc ctc gca ggc ttc cac tcc ctc aac 1171Gly Phe Lys Phe Gln Phe Ile Thr Leu Ala Gly Phe His Ser Leu Asn
345 350 355tac ggc atg ttc gac ctg gct tac gga tac gct cgc gaa ggc atg acc 1219Tyr Gly Met Phe Asp Leu Ala Tyr Gly Tyr Ala Arg Glu Gly Met Thr
360 365 370tcc ttc gtt gac ctg cag aac cgt gag ttc aag gca gct gaa gag cgt 1267Ser Phe Val Asp Leu Gln Asn Arg Glu Phe Lys Ala Ala Glu Glu Arg
375 380 385ggc ttc acc gct gtt aag cac cag cgt gag gtt ggc gca ggc tac ttc 1315Gly Phe Thr Ala Val Lys His Gln Arg Glu Val Gly Ala Gly Tyr Phe390 395 400 405gac cag atc gca acc acc gtt gac ccg aac tct tct acc acc gct ttg 1363Asp Gln Ile Ala Thr Thr Val Asp Pro Asn Ser Ser Thr Thr Ala Leu
410 415 420aag ggt tcc act gaa gaa ggc cag ttc cac aac taggacctac aggttctgac 1416Lys Gly Ser Thr Glu Glu Gly Gln Phe His Asn
425 430aat 1419<210>592<211>432<212>PRT<213>谷氨酸棒杆菌<400>592Met Ser Asn Val Gly Lys Pro Arg Thr Ala Gln Glu Ile Gln Gln Asp1 5 10 15Trp Asp Thr Asn Pro Arg Trp Asn Gly Ile Thr Arg Asp Tyr Thr Ala
20 25 30Asp Gln Val Ala Asp Leu Gln Gly Ser Val Ile Glu Glu His Thr Leu
35 40 45Ala Arg Arg Gly Ser Glu Ile Leu Trp Asp Ala Val Thr Gln Glu Gly
50 55 60Asp Gly Tyr Ile Asn Ala Leu Gly Ala Leu Thr Gly Asn Gln Ala Val65 70 75 80Gln Gln Val Arg Ala Gly Leu Lys Ala Val Tyr Leu Ser Gly Trp Gln
85 90 95Val Ala Gly Asp Ala Asn Leu Ser Gly His Thr Tyr Pro Asp Gln Ser
100 105 110Leu Tyr Pro Ala Asn Ser Val Pro Ser Val Val Arg Arg Ile Asn Asn
115 120 125Ala Leu Leu Arg Ser Asp Glu Ile Ala Arg Thr Glu Gly Asp Thr Ser
130 135 140Val Asp Asn Trp Val Val Pro Ile Val Ala Asp Gly Glu Ala Gly Phe145 150 155 160Gly Gly Ala Leu Asn Val Tyr Glu Leu Gln Lys Ala Met Ile Ala Ala
165 170 175Gly Ala Ala Gly Thr His Trp Glu Asp Gln Leu Ala Ser Glu Lys Lys
180 185 190Cys Gly His Leu Gly Gly Lys Val Leu Ile Pro Thr Gln Gln His Ile
195 200 205Arg Thr Leu Asn Ser Ala Arg Leu Ala Ala Asp Val Ala Asn Thr Pro
210 215 220Thr Val Val Ile Ala Arg Thr Asp Ala Glu Ala Ala Thr Leu Ile Thr225 230 235 240Ser Asp Val Asp Glu Arg Asp Gln Pro Phe Ile Thr Gly Glu Arg Thr
245 250 255Ala Glu Gly Tyr Tyr His Val Lys Asn Gly Leu Glu Pro Cys Ile Ala
260 265 270Arg Ala Lys Ser Tyr Ala Pro Tyr Ala Asp Met Ile Trp Met Glu Thr
275 280 285Gly Thr Pro Asp Leu Glu Leu Ala Lys Lys Phe Ala Glu Gly Val Arg
290 295 300Ser Glu Phe Pro Asp Gln Leu Leu Ser Tyr Asn Cys Ser Pro Ser Phe305 310 315 320Asn Trp Ser Ala His Leu Glu Ala Asp Glu Ile Ala Lys Phe Gln Lys
325 330 335Glu Leu Gly Ala Met Gly Phe Lys Phe Gln Phe Ile Thr Leu Ala Gly
340 345 350Phe His Ser Leu Asn Tyr Gly Met Phe Asp Leu Ala Tyr Gly Tyr Ala
355 360 365Arg Glu Gly Met Thr Ser Phe Val Asp Leu Gln Asn Arg Glu Phe Lys
370 375 380Ala Ala Glu Glu Arg Gly Phe Thr Ala Val Lys His Gln Arg Glu Val385 390 395 400Gly Ala Gly Tyr Phe Asp Gln Ile Ala Thr Thr Val Asp Pro Asn Ser
405 410 415Ser Thr Thr Ala Leu Lys Gly Ser Thr Glu Glu Gly Gln Phe His Asn
420 425 430<210>593<211>2340<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2317)<223>RXN02404<400>593gttttcacaa ccgttaacgg cgtagccaaa caagaaggat tcgcattctt ctggtttagg 60cacaggtcat ctaaaaccca tgctttaaaa ggagccttca atg act gaa cag gaa 115
Met Thr Glu Gln Glu
1 5ctg ttg tct gct cag act gcc gac aac gct gga act gac agc acc gaa 163Leu Leu Ser Ala Gln Thr Ala Asp Asn Ala Gly Thr Asp Ser Thr Glu
10 15 20cgc gtt gac gcg ggc gga atg cag gtt gca aaa gtt ctc tac gac ttt 211Arg Val Asp Ala Gly Gly Met Gln Val Ala Lys Val Leu Tyr Asp Phe
25 30 35gta acc gaa gcg gta ctc cct cgc gtg ggt gtg gat gcg gaa aag ttc 259Val Thr Glu Ala Val Leu Pro Arg Val Gly Val Asp Ala Glu Lys Phe
40 45 50tgg tcc gga ttc gcc gcc atc gcc cgg gac ctc acc cca cgc aac cgc 307Trp Ser Gly Phe Ala Ala Ile Ala Arg Asp Leu Thr Pro Arg Asn Arg
55 60 65gag ctg ctt gct cgc cgc gat gaa ctg cag atg ctt atc gac gac tac 355Glu Leu Leu Ala Arg Arg Asp Glu Leu Gln Met Leu Ile Asp Asp Tyr70 75 80 85cac cgc aac aac tcc ggc acc atc gac caa gag gcg tac gag gat ttc 403His Arg Asn Asn Ser Gly Thr Ile Asp Gln Glu Ala Tyr Glu Asp Phe
90 95 100ctc aaa gaa atc gga tac ttg gtt gag gag cca gaa gct gca gaa atc 451Leu Lys Glu Ile Gly Tyr Leu Val Glu Glu Pro Glu Ala Ala Glu Ile
105 110 115cgt acc caa aac gtc gat acg gaa atc tcc agc acc gca gga cct cag 499Arg Thr Gln Asn Val Asp Thr Glu Ile Ser Ser Thr Ala Gly Pro Gln
120 125 130ctg gtt gtt cca att ctg aac gca cgc ttc gcg ctg aac gct gcc aat 547Leu Val Val Pro Ile Leu Asn Ala Arg Phe Ala Leu Asn Ala Ala Asn
135 140 145gct cgc tgg ggt tcc ctc tac gat gcg ttg tac ggc acc aac gcc atc 595Ala Arg Trp Gly Ser Leu Tyr Asp Ala Leu Tyr Gly Thr Asn Ala Ile150 155 160 165cca gaa act gat ggc gct gaa aag ggc aag gag tac aac ccg gtc cgc 643Pro Glu Thr Asp Gly Ala Glu Lys Gly Lys Glu Tyr Asn Pro Val Arg
170 175 180ggc cag aag gtc atc gag tgg ggt cgt gaa ttc ctc gac agc gtt gtc 691Gly Gln Lys Val Ile Glu Trp Gly Arg Glu Phe Leu Asp Ser Val Val
185 190 195cca ctg gac ggt gct tcg cat gcc gat gtt gag aag tac aac atc acc 739Pro Leu Asp Gly Ala Ser His Ala Asp Val Glu Lys Tyr Asn Ile Thr
200 205 210gat gga aag ctt gca gcc cac att gga gat agc gtc tac cga ctg aaa 787Asp Gly Lys Leu Ala Ala His Ile Gly Asp Ser Val Tyr Arg Leu Lys
215 220 225aac cgt gaa tcc tac cgt ggc ttc acc ggc aac ttc ctt gat cca gaa 835Asn Arg Glu Ser Tyr Arg Gly Phe Thr Gly Asn Phe Leu Asp Pro Glu230 235 240 245gca atc ctg ctg gaa acc aac ggc ctg cac atc gag ctg cag atc gat 883Ala Ile Leu Leu Glu Thr Asn Gly Leu His Ile Glu Leu Gln Ile Asp
250 255 260cct gtc cac cca atc ggc aag gca gac aag act ggt ctc aaa gac atc 931Pro Val His Pro Ile Gly Lys Ala Asp Lys Thr Gly Leu Lys Asp Ile
265 270 275gtt ttg gaa tct gcg atc acc acg atc atg gac ttc gaa gac tcc gtt 979Val Leu Glu Ser Ala Ile Thr Thr Ile Met Asp Phe Glu Asp Ser Val
280 285 290gca gct gtt gat gct gaa gac aag acc tta ggt tac tct aac tgg ttc 1027Ala Ala Val Asp Ala Glu Asp Lys Thr Leu Gly Tyr Ser Asn Trp Phe
295 300 305gga ctc aac acc ggc gaa ctg aaa gaa gag atg tcc aag aac gga cgc 1075Gly Leu Asn Thr Gly Glu Leu Lys Glu Glu Met Ser Lys Asn Gly Arg310 315 320 325atc ttc acc cgt gag ctc aac aag gac cgc gtc tac att ggc cgc aat 1123Ile Phe Thr Arg Glu Leu Asn Lys Asp Arg Val Tyr Ile Gly Arg Asn
330 335 340ggt acc gag ctg gtt ctg cac ggt cgt tcc ctg ctg ttc gtc cgc aac 1171Gly Thr Glu Leu Val Leu His Gly Arg Ser Leu Leu Phe Val Arg Asn
345 350 355gtt ggt cac ctc atg caa aac cca tcc atc ttg att gat ggc gag gag 1219Val Gly His Leu Met Gln Asn Pro Ser Ile Leu Ile Asp Gly Glu Glu
360 365 370atc ttc gaa ggc atc atg gat gct gtc ttg acc act gtt tgt gcc atc 1267Ile Phe Glu Gly Ile Met Asp Ala Val Leu Thr Thr Val Cys Ala Ile
375 380 385cca gga att gct ccg cag aac aag atg cgc aat tcc cgc aag ggc tcc 1315Pro Gly Ile Ala Pro Gln Asn Lys Met Arg Asn Ser Arg Lys Gly Ser390 395 400 405atc tac atc gtg aag cct aag cag cac ggc cct gaa gaa gtc gcg ttc 1363Ile Tyr Ile Val Lys Pro Lys Gln His Gly Pro Glu Glu Val Ala Phe
410 415 420acc aac gag ctc ttc ggc cgc gtt gag gat ctg ctt gat ctg cca cgc 1411Thr Asn Glu Leu Phe Gly Arg Val Glu Asp Leu Leu Asp Leu Pro Arg
425 430 435cac acc ttg aag gtt ggt gtt atg gat gag gag cgt cgc acg tcc gtg 1459His Thr Leu Lys Val Gly Val Met Asp Glu Glu Arg Arg Thr Ser Val
440 445 450aac ctg gat gcc agc atc atg gaa gtt gct gac cgc ttg gca ttc atc 1507Asn Leu Asp Ala Ser Ile Met Glu Val Ala Asp Arg Leu Ala Phe Ile
455 460 465aac act ggc ttc ctg gac cgc acc ggc gat gaa atc cac acc tcc atg 1555Asn Thr Gly Phe Leu Asp Arg Thr Gly Asp Glu Ile His Thr Ser Met470 475 480 485gaa gca ggc gcc atg gtg cgc aag gct gat atg cag acc gca ccg tgg 1603Glu Ala Gly Ala Met Val Arg Lys Ala Asp Met Gln Thr Ala Pro Trp
490 495 500aag cag gcc tac gag aac aac aac gtt gat gca ggt att cag cgt ggt 1651Lys Gln Ala Tyr Glu Asn Asn Asn Val Asp Ala Gly Ile Gln Arg Gly
505 510 515ctt cct ggc aag gct cag atc ggt aag ggc atg tgg gcg atg act gaa 1699Leu Pro Gly Lys Ala Gln Ile Gly Lys Gly Met Trp Ala Met Thr Glu
520 525 530ctc atg gca gaa atg ctg gag aag aag atc ggc cag cca cgc gaa ggc 1747Leu Met Ala Glu Met Leu Glu Lys Lys Ile Gly Gln Pro Arg Glu Gly
535 540 545gcc aac act gca tgg gtt cct tca cca act ggt gcg acg ctg cac gca 1795Ala Asn Thr Ala Trp Val Pro Ser Pro Thr Gly Ala Thr Leu His Ala550 555 560 565acg cac tac cac ttg gtt gat gtg ttc aag gtt caa gac gaa ctg cgt 1843Thr His Tyr His Leu Val Asp Val Phe Lys Val Gln Asp Glu Leu Arg
570 575 580gct gcc ggc cgc cgc gac agc ctg cgc aac att ctc acc att tca acc 1891Ala Ala Gly Arg Arg Asp Ser Leu Arg Asn Ile Leu Thr Ile Ser Thr
585 590 595gca cca aac acc aat tgg tct gag gaa gag aag aag gaa gag atg gac 1939Ala Pro Asn Thr Asn Trp Ser Glu Glu Glu Lys Lys Glu Glu Met Asp
600 605 610aac aac tgc cag tcc atc ctc gga tac gtt gtg cgc tgg gtt gag cac 1987Asn Asn Cys Gln Ser Ile Leu Gly Tyr Val Val Arg Trp Val Glu His
615 620 625ggt gtt ggt tgc tcc aag gtt cca gac atc cat gac atc gac ctc atg 2035Gly Val Gly Cys Ser Lys Val Pro Asp Ile His Asp Ile Asp Leu Met630 635 640 645gaa gac cgc gca acg ctg cgt att tcc tcg cag atg ctg gcc aac tgg 2083Glu Asp Arg Ala Thr Leu Arg Ile Ser Ser Gln Met Leu Ala Asn Trp
650 655 660atc cgc cat gat gtt gtc tcg aag gag cag gtc ttg gag tca ctg gaa 2131Ile Arg His Asp Val Val Ser Lys Glu Gln Val Leu Glu Ser Leu Glu
665 670 675cga atg gca gtg gtc gtc gac aag caa aat gcg ggc gac gag gcc tac 2179Arg Met Ala Val Val Val Asp Lys Gln Asn Ala Gly Asp Glu Ala Tyr
680 685 690cgc gat atg gcg ccg aag tac gac gcc tcc ctc gcc ttc cag gcg gct 2227Arg Asp Met Ala Pro Lys Tyr Asp Ala Ser Leu Ala Phe Gln Ala Ala
695 700 705aag gac ttg att ttc gaa ggc acc aag tcc cca tcg ggc tac acc gag 2275Lys Asp Leu Ile Phe Glu Gly Thr Lys Ser Pro Ser Gly Tyr Thr Glu710 715 720 725ccc atc ttg cac gca cgc cgc cgc gag ttc aaa gca aaa aac 2317Pro Ile Leu His Ala Arg Arg Arg Glu Phe Lys Ala Lys Asn
730 735taagcacgct tttcgacgct tac 2340<210>594<211>739<212>PRT<213>谷氨酸棒杆菌<400>594Met Thr Glu Gln Glu Leu Leu Ser Ala Gln Thr Ala Asp Asn Ala Gly1 5 10 15Thr Asp Ser Thr Glu Arg Val Asp Ala Gly Gly Met Gln Val Ala Lys
20 25 30Val Leu Tyr Asp Phe Val Thr Glu Ala Val Leu Pro Arg Val Gly Val
35 40 45Asp Ala Glu Lys Phe Trp Ser Gly Phe Ala Ala Ile Ala Arg Asp Leu
50 55 60Thr Pro Arg Asn Arg Glu Leu Leu Ala Arg Arg Asp Glu Leu Gln Met65 70 75 80Leu Ile Asp Asp Tyr His Arg Asn Asn Ser Gly Thr Ile Asp Gln Glu
85 90 95Ala Tyr Glu Asp Phe Leu Lys Glu Ile Gly Tyr Leu Val Glu Glu Pro
100 105 110Glu Ala Ala Glu Ile Arg Thr Gln Asn Val Asp Thr Glu Ile Ser Ser
115 120 125Thr Ala Gly Pro Gln Leu Val Val Pro Ile Leu Asn Ala Arg Phe Ala
130 135 140Leu Asn Ala Ala Asn Ala Arg Trp Gly Ser Leu Tyr Asp Ala Leu Tyr145 150 155 160Gly Thr Asn Ala Ile Pro Glu Thr Asp Gly Ala Glu Lys Gly Lys Glu
165 170 175Tyr Asn Pro Val Arg Gly Gln Lys Val Ile Glu Trp Gly Arg Glu Phe
180 185 190Leu Asp Ser Val Val Pro Leu Asp Gly Ala Ser His Ala Asp Val Glu
195 200 205Lys Tyr Asn Ile Thr Asp Gly Lys Leu Ala Ala His Ile Gly Asp Ser
210 215 220Val Tyr Arg Leu Lys Asn Arg Glu Ser Tyr Arg Gly Phe Thr Gly Asn225 230 235 240Phe Leu Asp Pro Glu Ala Ile Leu Leu Glu Thr Asn Gly Leu His Ile
245 250 255Glu Leu Gln Ile Asp Pro Val His Pro Ile Gly Lys Ala Asp Lys Thr
260 265 270Gly Leu Lys Asp Ile Val Leu Glu Ser Ala Ile Thr Thr Ile Met Asp
275 280 285Phe Glu Asp Ser Val Ala Ala Val Asp Ala Glu Asp Lys Thr Leu Gly
290 295 300Tyr Ser Asn Trp Phe Gly Leu Asn Thr Gly Glu Leu Lys Glu Glu Met305 310 315 320Ser Lys Asn Gly Arg Ile Phe Thr Arg Glu Leu Asn Lys Asp Arg Val
325 330 335Tyr Ile Gly Arg Asn Gly Thr Glu Leu Val Leu His Gly Arg Ser Leu
340 345 350Leu Phe Val Arg Asn Val Gly His Leu Met Gln Asn Pro Ser Ile Leu
355 360 365Ile Asp Gly Glu Glu Ile Phe Glu Gly Ile Met Asp Ala Val Leu Thr
370 375 380Thr Val Cys Ala Ile Pro Gly Ile Ala Pro Gln Asn Lys Met Arg Asn385 390 395 400Ser Arg Lys Gly Ser Ile Tyr Ile Val Lys Pro Lys Gln His Gly Pro
405 410 415Glu Glu Val Ala Phe Thr Asn Glu Leu Phe Gly Arg Val Glu Asp Leu
420 425 430Leu Asp Leu Pro Arg His Thr Leu Lys Val Gly Val Met Asp Glu Glu
435 440 445Arg Arg Thr Ser Val Asn Leu Asp Ala Ser Ile Met Glu Val Ala Asp
450 455 460Arg Leu Ala Phe Ile Asn Thr Gly Phe Leu Asp Arg Thr Gly Asp Glu465 470 475 480Ile His Thr Ser Met Glu Ala Gly Ala Met Val Arg Lys Ala Asp Met
485 490 495Gln Thr Ala Pro Trp Lys Gln Ala Tyr Glu Asn Asn Asn Val Asp Ala
500 505 510Gly Ile Gln Arg Gly Leu Pro Gly Lys Ala Gln Ile Gly Lys Gly Met
515 520 525Trp Ala Met Thr Glu Leu Met Ala Glu Met Leu Glu Lys Lys Ile Gly
530 535 540Gln Pro Arg Glu Gly Ala Asn Thr Ala Trp Val Pro Ser Pro Thr Gly545 550 555 560Ala Thr Leu His Ala Thr His Tyr His Leu Val Asp Val Phe Lys Val
565 570 575Gln Asp Glu Leu Arg Ala Ala Gly Arg Arg Asp Ser Leu Arg Asn Ile
580 585 590Leu Thr Ile Ser Thr Ala Pro Asn Thr Asn Trp Ser Glu Glu Glu Lys
595 600 605Lys Glu Glu Met Asp Asn Asn Cys Gln Ser Ile Leu Gly Tyr Val Val
610 615 620Arg Trp Val Glu His Gly Val Gly Cys Ser Lys Val Pro Asp Ile His625 630 635 640Asp Ile Asp Leu Met Glu Asp Arg Ala Thr Leu Arg Ile Ser Ser Gln
645 650 655Met Leu Ala Asn Trp Ile Arg His Asp Val Val Ser Lys Glu Gln Val
660 665 670Leu Glu Ser Leu Glu Arg Met Ala Val Val Val Asp Lys Gln Asn Ala
675 680 685Gly Asp Glu Ala Tyr Arg Asp Met Ala Pro Lys Tyr Asp Ala Ser Leu
690 695 700Ala Phe Gln Ala Ala Lys Asp Leu Ile Phe Glu Gly Thr Lys Ser Pro705 710 715 720Ser Gly Tyr Thr Glu Pro Ile Leu His Ala Arg Arg Arg Glu Phe Lys
725 730 735Ala Lys Asn<210>595<211>2159<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2136)<223>FRXA02404<400>595atg cag gtt gca aaa gtt ctc tac gac ttt gta acc gaa gcg gta ctc 48Met Gln Val Ala Lys Val Leu Tyr Asp Phe Val Thr Glu Ala Val Leu1 5 10 15cct cgc gtg ggt gtg gat gcg gaa aag ttc tgg tcc gga ttc gcc gcc 96Pro Arg Val Gly Val Asp Ala Glu Lys Phe Trp Ser Gly Phe Ala Ala
20 25 30atc gcc cgg gac ctc acc cca cgc aac cgc gag ctg ctt gct cgc cgc 144Ile Ala Arg Asp Leu Thr Pro Arg Asn Arg Glu Leu Leu Ala Arg Arg
35 40 45gat gaa ctg cag atg ctt atc gac gac tac cac cgc aac aac tcc ggc 192Asp Glu Leu Gln Met Leu Ile Asp Asp Tyr His Arg Asn Asn Ser Gly
50 55 60acc atc gac caa gag gcg tac gag gat ttc ctc aaa gaa atc gga tac 240Thr Ile Asp Gln Glu Ala Tyr Glu Asp Phe Leu Lys Glu Ile Gly Tyr65 70 75 80ttg gtt gag gag cca gaa gct gca gaa atc cgt acc caa aac gtc gat 288Leu Val Glu Glu Pro Glu Ala Ala Glu Ile Arg Thr Gln Asn Val Asp
85 90 95acg gaa atc tcc agc acc gca gga cct cag ctg gtt gtt cca att ctg 336Thr Glu Ile Ser Ser Thr Ala Gly Pro Gln Leu Val Val Pro Ile Leu
100 105 110aac gca cgc ttc gcg ctg aac gct gcc aat gct cgc tgg ggt tcc ctc 384Asn Ala Arg Phe Ala Leu Asn Ala Ala Asn Ala Arg Trp Gly Ser Leu
115 120 125tac gat gcg ttg tac ggc acc aac gcc atc cca gaa act gat ggc gct 432Tyr Asp Ala Leu Tyr Gly Thr Asn Ala Ile Pro Glu Thr Asp Gly Ala
130 135 140gaa aag ggc aag gag tac aac ccg gtc cgc ggc cag aag gtc atc gag 480Glu Lys Gly Lys Glu Tyr Asn Pro Val Arg Gly Gln Lys Val Ile Glu145 150 155 160tgg ggt cgt gaa ttc ctc gac agc gtt gtc cca ctg gac ggt gct tcg 528Trp Gly Arg Glu Phe Leu Asp Ser Val Val Pro Leu Asp Gly Ala Ser
165 170 175cat gcc gat gtt gag aag tac aac atc acc gat gga aag ctt gca gcc 576His Ala Asp Val Glu Lys Tyr Asn Ile Thr Asp Gly Lys Leu Ala Ala
180 185 190cac att gga gat agc gtc tac cga ctg aaa aac cgt gaa tcc tac cgt 624His Ile Gly Asp Ser Val Tyr Arg Leu Lys Asn Arg Glu Ser Tyr Arg
195 200 205ggc ttc acc ggc aac ttc ctt gat cca gaa gca atc ctg ctg gaa acc 672Gly Phe Thr Gly Asn Phe Leu Asp Pro Glu Ala Ile Leu Leu Glu Thr
210 215 220aac ggc ctg cac atc gag ctg cag atc gat cct gtc cac cca atc ggc 720Asn Gly Leu His Ile Glu Leu Gln Ile Asp Pro Val His Pro Ile Gly225 230 235 240aag gca gac aag act ggt ctc aaa gac atc gtt ttg gaa tct gcg atc 768Lys Ala Asp Lys Thr Gly Leu Lys Asp Ile Val Leu Glu Ser Ala Ile
245 250 255acc acg atc atg gac ttc gaa gac tcc gtt gca gct gtt gat gct gaa 816Thr Thr Ile Met Asp Phe Glu Asp Ser Val Ala Ala Val Asp Ala Glu
260 265 270gac aag acc tta ggt tac tct aac tgg ttc gga ctc aac acc ggc gaa 864Asp Lys Thr Leu Gly Tyr Ser Asn Trp Phe Gly Leu Asn Thr Gly Glu
275 280 285ctg aaa gaa gag atg tcc aag aac gga cgc atc ttc acc cgt gag ctc 912Leu Lys Glu Glu Met Ser Lys Asn Gly Arg Ile Phe Thr Arg Glu Leu
290 295 300aac aag gac cgc gtc tac att ggc cgc aat ggt acc gag ctg gtt ctg 960Asn Lys Asp Arg Val Tyr Ile Gly Arg Asn Gly Thr Glu Leu Val Leu305 310 315 320cac ggt cgt tcc ctg ctg ttc gtc cgc aac gtt ggt cac ctc atg caa 1008His Gly Arg Ser Leu Leu Phe Val Arg Asn Val Gly His Leu Met Gln
325 330 335aac cca tcc atc ttg att gat ggc gag gag atc ttc gaa ggc atc atg 1056Asn Pro Ser Ile Leu Ile Asp Gly Glu Glu Ile Phe Glu Gly Ile Met
340 345 350gat gct gtc ttg acc act gtt tgt gcc atc cca gga att gct ccg cag 1104Asp Ala Val Leu Thr Thr Val Cys Ala Ile Pro Gly Ile Ala Pro Gln
355 360 365aac aag atg cgc aat tcc cgc aag ggc tcc atc tac atc gtg aag cct 1152Asn Lys Met Arg Asn Ser Arg Lys Gly Ser Ile Tyr Ile Val Lys Pro
370 375 380aag cag cac ggc cct gaa gaa gtc gcg ttc acc aac gag ctc ttc ggc 1200Lys Gln His Gly Pro Glu Glu Val Ala Phe Thr Asn Glu Leu Phe Gly385 390 395 400cgc gtt gag gat ctg ctt gat ctg cca cgc cac acc ttg aag gtt ggt 1248Arg Val Glu Asp Leu Leu Asp Leu Pro Arg His Thr Leu Lys Val Gly
405 410 415gtt atg gat gag gag cgt cgc acg tcc gtg aac ctg gat gcc agc atc 1296Val Met Asp Glu Glu Arg Arg Thr Ser Val Asn Leu Asp Ala Ser Ile
420 425 430atg gaa gtt gct gac cgc ttg gca ttc atc aac act ggc ttc ctg gac 1344Met Glu Val Ala Asp Arg Leu Ala Phe Ile Asn Thr Gly Phe Leu Asp
435 440 445cgc acc ggc gat gaa atc cac acc tcc atg gsa gca ggc gcc atg gtg 1392Arg Thr Gly Asp Glu Ile His Thr Ser Met Glu Ala Gly Ala Met Val
450 455 460cgc aag gct gat atg cag acc gca ccg tgg aag cag gcc tac gag aac 1440Arg Lys Ala Asp Met Gln Thr Ala Pro Trp Lys Gln Ala Tyr Glu Asn465 470 475 480aac aac gtt gat gca ggt att cag cgt ggt ctt cct ggc aag gct cag 1488Asn Asn Val Asp Ala Gly Ile Gln Arg Gly Leu Pro Gly Lys Ala Gln
485 490 495atc ggt aag ggc atg tgg gcg atg act gaa ctc atg gca gaa atg ctg 1536Ile Gly Lys Gly Met Trp Ala Met Thr Glu Leu Met Ala Glu Met Leu
500 505 510gag aag aag atc ggc cag cca cgc gaa ggc gcc aac act gca tgg gtt 1584Glu Lys Lys Ile Gly Gln Pro Arg Glu Gly Ala Asn Thr Ala Trp Val
515 520 525cct tca cca act ggt gcg acg ctg cac gca acg cac tac cac ttg gtt 1632Pro Ser Pro Thr Gly Ala Thr Leu His Ala Thr His Tyr His Leu Val
530 535 540gat gtg ttc aag gtt caa gac gaa ctg cgt gct gcc ggc cgc cgc gac 1680Asp Val Phe Lys Val Gln Asp Glu Leu Arg Ala Ala Gly Arg Arg Asp545 550 555 560agc ctg cgc aac att ctc acc att tca acc gca cca aac acc aat tgg 1728Ser Leu Arg Asn Ile Leu Thr Ile Ser Thr Ala Pro Asn Thr Asn Trp
565 570 575tct gag gaa gag aag aag gaa gag atg gac aac aac tgc cag tcc atc 1776Ser Glu Glu Glu Lys Lys Glu Glu Met Asp Asn Asn Cys Gln Ser Ile
580 585 590ctc gga tac gtt gtg cgc tgg gtt gag cac ggt gtt ggt tgc tcc aag 1824Leu Gly Tyr Val Val Arg Trp Val Glu His Gly Val Gly Cys Ser Lys
595 600 605gtt cca gac atc cat gac atc gac ctc atg gaa gac cgc gca acg ctg 1872Val Pro Asp Ile His Asp Ile Asp Leu Met Glu Asp Arg Ala Thr Leu
610 615 620cgt att tcc tcg cag atg ctg gcc aac tgg atc cgc cat gat gtt gtc 1920Arg Ile Ser Ser Gln Met Leu Ala Asn Trp Ile Arg His Asp Val Val625 630 635 640tcg aag gag cag gtc ttg gag tca ctg gaa cga atg gca gtg gtc gtc 1968Ser Lys Glu Gln Val Leu Glu Ser Leu Glu Arg Met Ala Val Val Val
645 650 655gac aag caa aat gcg ggc gac gag gcc tac cgc gat atg gcg ccg aag 2016Asp Lys Gln Asn Ala Gly Asp Glu Ala Tyr Arg Asp Met Ala Pro Lys
660 665 670tac gac gcc tcc ctc gcc ttc cag gcg gct aag gac ttg att ttc gaa 2064Tyr Asp Ala Ser Leu Ala Phe Gln Ala Ala Lys Asp Leu Ile Phe Glu
675 680 685ggc acc aag tcc cca tcg ggc tac acc gag ccc atc ttg cac gca cgc 2112Gly Thr Lys Ser Pro Ser Gly Tyr Thr Glu Pro Ile Leu His Ala Arg
690 695 700cgc cgc gag ttc aaa gca aaa aac taagcacgct tttcgacgct tac 2159Arg Arg Glu Phe Lys Ala Lys Asn705 710<210>596<211>712<212>PRT<213>谷氨酸棒杆菌<400>596Met Gln Val Ala Lys Val Leu Tyr Asp Phe Val Thr Glu Ala Val Leu1 5 10 15Pro Arg Val Gly Val Asp Ala Glu Lys Phe Trp Ser Gly Phe Ala Ala
20 25 30Ile Ala Arg Asp Leu Thr Pro Arg Asn Arg Glu Leu Leu Ala Arg Arg
35 40 45Asp Glu Leu Gln Met Leu Ile Asp Asp Tyr His Arg Asn Asn Ser Gly
50 55 60Thr Ile Asp Gln Glu Ala Tyr Glu Asp Phe Leu Lys Glu Ile Gly Tyr65 70 75 80Leu Val Glu Glu Pro Glu Ala Ala Glu Ile Arg Thr Gln Asn Val Asp
85 90 95Thr Glu Ile Ser Ser Thr Ala Gly Pro Gln Leu Val Val Pro Ile Leu
100 105 110Asn Ala Arg Phe Ala Leu Asn Ala Ala Asn Ala Arg Trp Gly Ser Leu
115 120 125Tyr Asp Ala Leu Tyr Gly Thr Asn Ala Ile Pro Glu Thr Asp Gly Ala
130 135 140Glu Lys Gly Lys Glu Tyr Asn Pro Val Arg Gly Gln Lys Val Ile Glu145 150 155 160Trp Gly Arg Glu Phe Leu Asp Ser Val Val Pro Leu Asp Gly Ala Ser
165 170 175His Ala Asp Val Glu Lys Tyr Asn Ile Thr Asp Gly Lys Leu Ala Ala
180 185 190His Ile Gly Asp Ser Val Tyr Arg Leu Lys Asn Arg Glu Ser Tyr Arg
195 200 205Gly Phe Thr Gly Asn Phe Leu Asp Pro Glu Ala Ile Leu Leu Glu Thr
210 215 220Asn Gly Leu His Ile Glu Leu Gln Ile Asp Pro Val His Pro Ile Gly225 230 235 240Lys Ala Asp Lys Thr Gly Leu Lys Asp Ile Val Leu Glu Ser Ala Ile
245 250 255Thr Thr Ile Met Asp Phe Glu Asp Ser Val Ala Ala Val Asp Ala Glu
260 265 270Asp Lys Thr Leu Gly Tyr Ser Asn Trp Phe Gly Leu Asn Thr Gly Glu
275 280 285Leu Lys Glu Glu Met Ser Lys Asn Gly Arg Ile Phe Thr Arg Glu Leu
290 295 300Asn Lys Asp Arg Val Tyr Ile Gly Arg Asn Gly Thr Glu Leu Val Leu305 310 315 320His Gly Arg Ser Leu Leu Phe Val Arg Asn Val Gly His Leu Met Gln
325 330 335Asn Pro Ser Ile Leu Ile Asp Gly Glu Glu Ile Phe Glu Gly Ile Met
340 345 350Asp Ala Val Leu Thr Thr Val Cys Ala Ile Pro Gly Ile Ala Pro Gln
355 360 365Asn Lys Met Arg Asn Ser Arg Lys Gly Ser Ile Tyr Ile Val Lys Pro
370 375 380Lys Gln His Gly Pro Glu Glu Val Ala Phe Thr Asn Glu Leu Phe Gly385 390 395 400Arg Val Glu Asp Leu Leu Asp Leu Pro Arg His Thr Leu Lys Val Gly
405 410 415Val Met Asp Glu Glu Arg Arg Thr Ser Val Asn Leu Asp Ala Ser Ile
420 425 430Met Glu Val Ala Asp Arg Leu Ala Phe Ile Asn Thr Gly Phe Leu Asp
435 440 445Arg Thr Gly Asp Glu Ile His Thr Ser Met Glu Ala Gly Ala Met Val
450 455 460Arg Lys Ala Asp Met Gln Thr Ala Pro Trp Lys Gln Ala Tyr Glu Asn465 470 475 480Asn Asn Val Asp Ala Gly Ile Gln Arg Gly Leu Pro Gly Lys Ala Gln
485 490 495Ile Gly Lys Gly Met Trp Ala Met Thr Glu Leu Met Ala Glu Met Leu
500 505 510Glu Lys Lys Ile Gly Gln Pro Arg Glu Gly Ala Asn Thr Ala Trp Val
515 520 525Pro Ser Pro Thr Gly Ala Thr Leu His Ala Thr His Tyr His Leu Val
530 535 540Asp Val Phe Lys Val Gln Asp Glu Leu Arg Ala Ala Gly Arg Arg Asp545 550 555 560Ser Leu Arg Asn Ile Leu Thr Ile Ser Thr Ala Pro Asn Thr Asn Trp
565 570 575Ser Glu Glu Glu Lys Lys Glu Glu Met Asp Asn Asn Cys Gln Ser Ile
580 585 590Leu Gly Tyr Val Val Arg Trp Val Glu His Gly Val Gly Cys Ser Lys
595 600 605Val Pro Asp Ile His Asp Ile Asp Leu Met Glu Asp Arg Ala Thr Leu
610 615 620Arg Ile Ser Ser Gln Met Leu Ala Asn Trp Ile Arg His Asp Val Val625 630 635 640Ser Lys Glu Gln Val Leu Glu Ser Leu Glu Arg Met Ala Val Val Val
645 650 655Asp Lys Gln Asn Ala Gly Asp Glu Ala Tyr Arg Asp Met Ala Pro Lys
660 665 670Tyr Asp Ala Ser Leu Ala Phe Gln Ala Ala Lys Asp Leu Ile Phe Glu
675 680 685Gly Thr Lys Ser Pro Ser Gly Tyr Thr Glu Pro Ile Leu His Ala Arg
690 695 700Arg Arg Glu Phe Lys Ala Lys Asn705 710<210>597<211>873<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(850)<223>RXA01089<400>597aaaaattcgg ttcacaaaag gtttatcagt ccagcttaaa cccatcgcac gggcgggaga 60attagactca aggcacatca cattgaagga gcttcttatc ttg tct cga ttt gct 115
Leu Ser Arg Phe Ala
1 5gcc aac ctg tca ttg act ttt act gag cta gat ttc ctg gat cgt ttt 163Ala Asn Leu Ser Leu Thr Phe Thr Glu Leu Asp Phe Leu Asp Arg Phe
10 15 20gat gcc gct tcg aag cat gct ttc agt gcc gtg gag ttt cag tac cct 211Asp Ala Ala Ser Lys His Ala Phe Ser Ala Val Glu Phe Gln Tyr Pro
25 30 35tac gat ttc gat gtt caa gag att aaa cag cgt gct gat tcc gca ggt 259Tyr Asp Phe Asp Val Gln Glu Ile Lys Gln Arg Ala Asp Ser Ala Gly
40 45 50ctg ccc att gaa ctg ttc aat gcc cca cct ggg gat act ttt ggt ctt 307Leu Pro Ile Glu Leu Phe Asn Ala Pro Pro Gly Asp Thr Phe Gly Leu
55 60 65gcg gca ctg gct tcc cct gaa gac ttt caa caa tcc atc gag cag gcc 355Ala Ala Leu Ala Ser Pro Glu Asp Phe Gln Gln Ser Ile Glu Gln Ala70 75 80 85atc acg tac gcc aca gtg ttg aag cca aag aag atg cat gtc atg gct 403Ile Thr Tyr Ala Thr Val Leu Lys Pro Lys Lys Met His Val Met Ala
90 95 100ggc atc gcg gac gta acc tca gaa acc acg gcg cgc tat gtg gag aat 451Gly Ile Ala Asp Val Thr Ser Glu Thr Thr Ala Arg Tyr Val Glu Asn
105 110 115att cgc tgg gct gcg cag caa cta gac aag ctc gac gtt gtc gtt gtt 499Ile Arg Trp Ala Ala Gln Gln Leu Asp Lys Leu Asp Val Val Val Val
120 125 130att gaa cca att aat cac tat tcg gtt ccc ggt tat ttc ctg cac act 547Ile Glu Pro Ile Asn His Tyr Ser Val Pro Gly Tyr Phe Leu His Thr
135 140 145tta gag cag gcg tat tgg ctt atc gac agc att gcc cac ccc aat gtg 595Leu Glu Gln Ala Tyr Trp Leu Ile Asp Ser Ile Ala His Pro Asn Val150 155 160 165aag atc tta ttc gat act ttc cac ctt cag cag att cat ggc aat ctc 643Lys Ile Leu Phe Asp Thr Phe His Leu Gln Gln Ile His Gly Asn Leu
170 175 180acc cgc cgc ctg cgc gag gtt cat ggc gca ggt ctt ttg gga cac gtg 691Thr Arg Arg Leu Arg Glu Val His Gly Ala Gly Leu Leu Gly His Val
185 190 195caa gtg gcc tca gtt cct gat cga cac gaa cct ggc act ggc gaa gtc 739Gln Val Ala Ser Val Pro Asp Arg His Glu Pro Gly Thr Gly Glu Val
200 205 210aat gcg gcg tat atc ttc caa ctc cta agc gaa ctg gga tat gac ggt 787Asn Ala Ala Tyr Ile Phe Gln Leu Leu Ser Glu Leu Gly Tyr Asp Gly
215 220 225gtc atc gct ggc gaa tac cac cct gct ggt gaa act aca gcc ggt ttg 835Val Ile Ala Gly Glu Tyr His Pro Ala Gly Glu Thr Thr Ala Gly Leu230 235 240 245ggc tgg ttg gag ctc tagatcgtaa gtggtgtcgt acc 873Gly Trp Leu Glu Leu
250<210>598<211>250<212>PRT<213>谷氨酸棒杆菌<400>598Leu Ser Arg Phe Ala Ala Asn Leu Ser Leu Thr Phe Thr Glu Leu Asp1 5 10 15Phe Leu Asp Arg Phe Asp Ala Ala Ser Lys His Ala Phe Ser Ala Val
20 25 30Glu Phe Gln Tyr Pro Tyr Asp Phe Asp Val Gln Glu Ile Lys Gln Arg
35 40 45Ala Asp Ser Ala Gly Leu Pro Ile Glu Leu Phe Asn Ala Pro Pro Gly
50 55 60Asp Thr Phe Gly Leu Ala Ala Leu Ala Ser Pro Glu Asp Phe Gln Gln65 70 75 80Ser Ile Glu Gln Ala Ile Thr Tyr Ala Thr Val Leu Lys Pro Lys Lys
85 90 95Met His Val Met Ala Gly Ile Ala Asp Val Thr Ser Glu Thr Thr Ala
100 105 110Arg Tyr Val Glu Asn Ile Arg Trp Ala Ala Gln Gln Leu Asp Lys Leu
115 120 125Asp Val Val Val Val Ile Glu Pro Ile Asn His Tyr Ser Val Pro Gly
130 135 140Tyr Phe Leu His Thr Leu Glu Gln Ala Tyr Trp Leu Ile Asp Ser Ile145 150 155 160Ala His Pro Asn Val Lys Ile Leu Phe Asp Thr Phe His Leu Gln Gln
165 170 175Ile His Gly Asn Leu Thr Arg Arg Leu Arg Glu Val His Gly Ala Gly
180 185 190Leu Leu Gly His Val Gln Val Ala Ser Val Pro Asp Arg His Glu Pro
195 200 205Gly Thr Gly Glu Val Asn Ala Ala Tyr Ile Phe Gln Leu Leu Ser Glu
210 215 220Leu Gly Tyr Asp Gly Val Ile Ala Gly Glu Tyr His Pro Ala Gly Glu225 230 235 240Thr Thr Ala Gly Len Gly Trp Leu Glu Leu
245 250<210>599<211>897<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(874)<223>RXA01886<400>599gcgaagctgc acgcaccgca ctactcatcg cactcggcgc catccgaagc gtagaaaccg 60gcgcaaccat caaccttgct gaaagcatcg aggtttaacc atg act ttt aaa ctc 115
Met Thr Phe Lys Leu
1 5gca gca tgc gca gag atg atc tac cag gac ctg cct ttc gag gag agg 163Ala Ala Cys Ala Glu Met Ile Tyr Gln Asp Leu Pro Phe Glu Glu Arg
10 15 20gtc aag acg atc tct gat cag gga ttc ctc gtg gaa att tgg gac tgg 211Val Lys Thr Ile Ser Asp Gln Gly Phe Leu Val Glu Ile Trp Asp Trp
25 30 35tcc aca aaa gac atc gat gcg ctc gtg gca aca ggc gcg gaa ttt tcc 259Ser Thr Lys Asp Ile Asp Ala Leu Val Ala Thr Gly Ala Glu Phe Ser
40 45 50tcc atg acg ggc tac ctg cgc ggg gat ctg att act gaa cag ggc cgc 307Ser Met Thr Gly Tyr Leu Arg Gly Asp Leu Ile Thr Glu Gln Gly Arg
55 60 65gcg gag ctc ttg gca acc gct tcg gag tcc ttg gcg gtg gcg gaa aag 355Ala Glu Leu Leu Ala Thr Ala Ser Glu Ser Leu Ala Val Ala Glu Lys70 75 80 85ctc aac tgc ccc cgg ctg aat ctg cat gga act ggc ctt gga ccg cag 403Leu Asn Cys Pro Arg Leu Asn Leu His Gly Thr Gly Leu Gly Pro Gln
90 95 100gga cta cct gtt act ccc att gaa gtg gtt acc cca gaa atg tgg ctc 451Gly Leu Pro Val Thr Pro Ile Glu Val Val Thr Pro Glu Met Trp Leu
105 110 115tac gct gct gaa acg ctc cgc cag atc gct gag ctg ggg gag cgc gca 499Tyr Ala Ala Glu Thr Leu Arg Gln Ile Ala Glu Leu Gly Glu Arg Ala
120 125 130ggc aag gtt ttc gtg ctg gaa aac ctc aac ctc gca gtc gat cac ccc 547Gly Lys Val Phe Val Leu Glu Asn Leu Asn Leu Ala Val Asp His Pro
135 140 145ggc act cct ttt gcc aag gcc act gac act ttg gcg ctg gtc aag gct 595Gly Thr Pro Phe Ala Lys Ala Thr Asp Thr Leu Ala Leu Val Lys Ala150 155 160 165gtc aat cac ccg aat ctg cgc ctc aac ctg gat ttg tac cac gcc cag 643Val Asn His Pro Asn Leu Arg Leu Asn Leu Asp Leu Tyr His Ala Gln
170 175 180att ggc gaa gga aac ctc att gag ctg ctc cgt gag gcg cag cca ttc 691Ile Gly Glu Gly Asn Leu Ile Glu Leu Leu Arg Glu Ala Gln Pro Phe
185 190 195atc ggc gaa atc cag gtt gcc gat gtc ccc ggc cgc atg gaa ccc ggc 739Ile Gly Glu Ile Gln Val Ala Asp Val Pro Gly Arg Met Glu Pro Gly
200 205 210acc ggc gag atc aac tac cag ggc gtc gcg aaa gct ctc gcc gcg atg 787Thr Gly Glu Ile Asn Tyr Gln Gly Val Ala Lys Ala Leu Ala Ala Met
215 220 225ggc tac gac ggc gtc atc ggc atg gag gcg tgg gca tcg ggc gac tcc 835Gly Tyr Asp Gly Val Ile Gly Met Glu Ala Trp Ala Ser Gly Asp Ser230 235 240 245agc gac gcg ctg cag gcg ttg aag tca gcg ttc acg gtc taaattgctt 884Ser Asp Ala Leu Gln Ala Leu Lys Ser Ala Phe Thr Val
250 255atcgacgcac ccc 897<210>600<211>258<212>PRT<213>谷氨酸棒杆菌<400>600Met Thr Phe Lys Leu Ala Ala Cys Ala Glu Met Ile Tyr Gln Asp Leu1 5 10 15Pro Phe Glu Glu Arg Val Lys Thr Ile Ser Asp Gln Gly Phe Leu Val
20 25 30Glu Ile Trp Asp Trp Ser Thr Lys Asp Ile Asp Ala Leu Val Ala Thr
35 40 45Gly Ala Glu Phe Ser Ser Met Thr Gly Tyr Leu Arg Gly Asp Leu Ile
50 55 60Thr Glu Gln Gly Arg Ala Glu Leu Leu Ala Thr Ala Ser Glu Ser Leu65 70 75 80Ala Val Ala Glu Lys Leu Asn Cys Pro Arg Leu Asn Leu His Gly Thr
85 90 95Gly Leu Gly Pro Gln Gly Leu Pro Val Thr Pro Ile Glu Val Val Thr
100 105 110Pro Glu Met Trp Leu Tyr Ala Ala Glu Thr Leu Arg Gln Ile Ala Glu
115 120 125Leu Gly Glu Arg Ala Gly Lys Val Phe Val Leu Glu Asn Leu Asn Leu
130 135 140Ala Val Asp His Pro Gly Thr Pro Phe Ala Lys Ala Thr Asp Thr Leu145 150 155 160Ala Leu Val Lys Ala Val Asn His Pro Asn Leu Arg Leu Asn Leu Asp
165 170 175Leu Tyr His Ala Gln Ile Gly Glu Gly Asn Leu Ile Glu Leu Leu Arg
180 185 190Glu Ala Gln Pro Phe Ile Gly Glu Ile Gln Val Ala Asp Val Pro Gly
195 200 205Arg Met Glu Pro Gly Thr Gly Glu Ile Asn Tyr Gln Gly Val Ala Lys
210 215 220Ala Leu Ala Ala Met Gly Tyr Asp Gly Val Ile Gly Met Glu Ala Trp225 230 235 240Ala Ser Gly Asp Ser Ser Asp Ala Leu Gln Ala Leu Lys Ser Ala Phe
245 250 255Thr Val<210>601<211>1575<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(41)..(1552)<223>RXN03117<400>601tgtgcaacat tagttcgtta agaagagtca cattccagcc atg att acc cac gaa 55
Met Ile Thr His Glu
1 5gtg cgc acc cac cgt tct gcg gaa gag ttc ccg tac aag aag cac ctg 103Val Arg Thr His Arg Ser Ala Glu Glu Phe Pro Tyr Lys Lys His Leu
10 15 20gct cac aag atg gct cgc gtt gca gcc gac cca gtt gag gtt gct gcg 151Ala His Lys Met Ala Arg Val Ala Ala Asp Pro Val Glu Val Ala Ala
25 30 35gac act cag gaa atg atc atc acc cgc atc atc gac aat gca tcg gtg 199Asp Thr Gln Glu Met Ile Ile Thr Arg Ile Ile Asp Asn Ala Ser Val
40 45 50cag gca gct tcc gtg ttg cgt cga cca gtt agc tct gcc cgt gcg atg 247Gln Ala Ala Ser Val Leu Arg Arg Pro Val Ser Ser Ala Arg Ala Met
55 60 65gca cag gtc agg cca gtt acc gat ggt cgg ggt gca tct gtt ttc ggt 295Ala Gln Val Arg Pro Val Thr Asp Gly Arg Gly Ala Ser Val Phe Gly70 75 80 85ctg cca gga cgt tat gcc gcg gaa tgg gct gcg ctt gct aac ggc act 343Leu Pro Gly Arg Tyr Ala Ala Glu Trp Ala Ala Leu Ala Asn Gly Thr
90 95 100gcg gtg cgt gag ctt gat ttc cat gac acg ttc ctc gct gcg gaa tac 391Ala Val Arg Glu Leu Asp Phe His Asp Thr Phe Leu Ala Ala Glu Tyr
105 110 115tcc cac cca gga gat aac att cct ccg att ttg gct gca gca cag cag 439Ser His Pro Gly Asp Asn Ile Pro Pro Ile Leu Ala Ala Ala Gln Gln
120 125 130gct gga aaa ggt ggc aag gat ctg atc cgt ggc atc gct act ggg tat 487Ala Gly Lys Gly Gly Lys Asp Leu Ile Arg Gly Ile Ala Thr Gly Tyr
135 140 145gag att cag gtt aac ttg gtg cgt gga atg tgc ctg cat gag cac aag 535Glu Ile Gln Val Asn Leu Val Arg Gly Met Cys Leu His Glu His Lys150 155 160 165att gat cac gtt gct cat ctt gga cca tca gcg gct gct ggt atc gga 583Ile Asp His Val Ala His Leu Gly Pro Ser Ala Ala Ala Gly Ile Gly
170 175 180acc ttg cta gac cta gat gtg gac acc atc tac cag gca att ggt cag 631Thr Leu Leu Asp Leu Asp Val Asp Thr Ile Tyr Gln Ala Ile Gly Gln
185 190 195gca ttg cac acc acc acg gcg acg agg cag tcc cgt aaa ggt gcg att 679Ala Leu His Thr Thr Thr Ala Thr Arg Gln Ser Arg Lys Gly Ala Ile
200 205 210tct tca tgg aag gca ttt gct cct gcg ttt gcg ggc aag atg tcc atc 727Ser Ser Trp Lys Ala Phe Ala Pro Ala Phe Ala Gly Lys Met Ser Ile
215 220 225gag gca gta gat cgc gca atg cgt ggc gag ggc gca ccg tca cca atc 775Glu Ala Val Asp Arg Ala Met Arg Gly Glu Gly Ala Pro Ser Pro Ile230 235 240 245tgg gaa ggc gaa gac ggc gta atc gcg tgg ctg ctg tcc ggt ctt gat 823Trp Glu Gly Glu Asp Gly Val Ile Ala Trp Leu Leu Ser Gly Leu Asp
250 255 260cac atc tac acc att cct ttg cct gca gaa ggt gaa gcc aaa cga gca 871His Ile Tyr Thr Ile Pro Leu Pro Ala Glu Gly Glu Ala Lys Arg Ala
265 270 275atc ttg gat acc tac acc aag gaa cac tcg gcg gaa tac cag tca cag 919Ile Leu Asp Thr Tyr Thr Lys Glu His Ser Ala Glu Tyr Gln Ser Gln
280 285 290gca ccg atc gac ttg gcg cgc agc atg ggg gag aag ctg gca gca cag 967Ala Pro Ile Asp Leu Ala Arg Ser Met Gly Glu Lys Leu Ala Ala Gln
295 300 305ggc ttg gac ctg cgt gat gtg gac tcc atc gtt ttg cac acc tcc cac 1015Gly Leu Asp Leu Arg Asp Val Asp Ser Ile Val Leu His Thr Ser His310 315 320 325cac act cac tac gtg atc ggc acc gga tct aat gat cca cag aag ttc 1063His Thr His Tyr Val Ile Gly Thr Gly Ser Asn Asp Pro Gln Lys Phe
330 335 340gat cca gat gca tcg cga gaa acc ctt gat cac tcc atc atg tac att 1111Asp Pro Asp Ala Ser Arg Glu Thr Leu Asp His Ser Ile Met Tyr Ile
345 350 355ttc gct gtc gcg ctg aag gat cgc gcg tgg cac cac gag cgt tcc tat 1159Phe Ala Val Ala Leu Lys Asp Arg Ala Trp His His Glu Arg Ser Tyr
360 365 370gct cct gag cga gcc cac cgc cga gag acc atc gag ctg tgg aac aag 1207Ala Pro Glu Arg Ala His Arg Arg Glu Thr Ile Glu Leu Trp Asn Lys
375 380 385att tcc acg gtg gag gat cct gaa tgg acc agg cgt tac cac tcc gtt 1255Ile Ser Thr Val Glu Asp Pro Glu Trp Thr Arg Arg Tyr His Ser Val390 395 400 405gat cct gca gaa aag gcc ttc ggc gca cgc gca gtg atc acc ttc aag 1303Asp Pro Ala Glu Lys Ala Phe Gly Ala Arg Ala Val Ile Thr Phe Lys
410 415 420gat gga acc gtc gtg gaa gat gaa ctg gct gtg gcg aat gcg cat cct 1351Asp Gly Thr Val Val Glu Asp Glu Leu Ala Val Ala Asn Ala His Pro
425 430 435ctg gga gca cgg cct ttc gct agg gag cag tac att cag aaa ttc cgc 1399Leu Gly Ala Arg Pro Phe Ala Arg Glu Gln Tyr Ile Gln Lys Phe Arg
440 445 450acc ttg gct gaa ggt gtt gtg tcc gaa aag gaa cag gat cgc ttc ttg 1447Thr Leu Ala Glu Gly Val Val Ser Glu Lys Glu Gln Asp Arg Phe Leu
455 460 465gat gcg gca cag cgt acg cac gag ctt gag gat ctt tca gaa ctc aac 1495Asp Ala Ala Gln Arg Thr His Glu Leu Glu Asp Leu Ser Glu Leu Asn470 475 480 485att gaa ttg gat gcc gat att ttg gcc aag gct cct gtg att ccg gaa 1543Ile Glu Leu Asp Ala Asp Ile Leu Ala Lys Ala Pro Val Ile Pro Glu
490 495 500gga ctg ttc tgatggcggg tttgttttcc tct 1575Gly Leu Phe<210>602<211>504<212>PRT<213>谷氨酸棒杆菌<400>602Met Ile Thr His Glu Val Arg Thr His Arg Ser Ala Glu Glu Phe Pro1 5 10 15Tyr Lys Lys His Leu Ala His Lys Met Ala Arg Val Ala Ala Asp Pro
20 25 30Val Glu Val Ala Ala Asp Thr Gln Glu Met Ile Ile Thr Arg Ile Ile
35 40 45Asp Asn Ala Ser Val Gln Ala Ala Ser Val Leu Arg Arg Pro Val Ser
50 55 60Ser Ala Arg Ala Met Ala Gln Val Arg Pro Val Thr Asp Gly Arg Gly65 70 75 80Ala Ser Val Phe Gly Leu Pro Gly Arg Tyr Ala Ala Glu Trp Ala Ala
85 90 95Leu Ala Asn Gly Thr Ala Val Arg Glu Leu Asp Phe His Asp Thr Phe
100 105 110Leu Ala Ala Glu Tyr Ser His Pro Gly Asp Asn Ile Pro Pro Ile Leu
115 120 125Ala Ala Ala Gln Gln Ala Gly Lys Gly Gly Lys Asp Leu Ile Arg Gly
130 135 140Ile Ala Thr Gly Tyr Glu Ile Gln Val Asn Leu Val Arg Gly Met Cys145 150 155 160Leu His Glu His Lys Ile Asp His Val Ala His Leu Gly Pro Ser Ala
165 170 175Ala Ala Gly Ile Gly Thr Leu Leu Asp Leu Asp Val Asp Thr Ile Tyr
180 185 190Gln Ala Ile Gly Gln Ala Leu His Thr Thr Thr Ala Thr Arg Gln Ser
195 200 205Arg Lys Gly Ala Ile Ser Ser Trp Lys Ala Phe Ala Pro Ala Phe Ala
210 215 220Gly Lys Met Ser Ile Glu Ala Val Asp Arg Ala Met Arg Gly Glu Gly225 230 235 240Ala Pro Ser Pro Ile Trp Glu Gly Glu Asp Gly Val Ile Ala Trp Leu
245 250 255Leu Ser Gly Leu Asp His Ile Tyr Thr Ile Pro Leu Pro Ala Glu Gly
260 265 270Glu Ala Lys Arg Ala Ile Leu Asp Thr Tyr Thr Lys Glu His Ser Ala
275 280 285Glu Tyr Gln Ser Gln Ala Pro Ile Asp Leu Ala Arg Ser Met Gly Glu
290 295 300Lys Leu Ala Ala Gln Gly Leu Asp Leu Arg Asp Val Asp Ser Ile Val305 310 315 320Leu His Thr Ser His His Thr His Tyr Val Ile Gly Thr Gly Ser Asn
325 330 335Asp Pro Gln Lys Phe Asp Pro Asp Ala Ser Arg Glu Thr Leu Asp His
340 345 350Ser Ile Met Tyr Ile Phe Ala Val Ala Leu Lys Asp Arg Ala Trp His
355 360 365His Glu Arg Ser Tyr Ala Pro Glu Arg Ala His Arg Arg Glu Thr Ile
370 375 380Glu Leu Trp Asn Lys Ile Ser Thr Val Glu Asp Pro Glu Trp Thr Arg385 390 395 400Arg Tyr His Ser Val Asp Pro Ala Glu Lys Ala Phe Gly Ala Arg Ala
405 410 415Val Ile Thr Phe Lys Asp Gly Thr Val Val Glu Asp Glu Leu Ala Val
420 425 430Ala Asn Ala His Pro Leu Gly Ala Arg Pro Phe Ala Arg Glu Gln Tyr
435 440 445Ile Gln Lys Phe Arg Thr Leu Ala Glu Gly Val Val Ser Glu Lys Glu
450 455 460Gln Asp Arg Phe Leu Asp Ala Ala Gln Arg Thr His Glu Leu Glu Asp465 470 475 480Leu Ser Glu Leu Asn Ile Glu Leu Asp Ala Asp Ile Leu Ala Lys Ala
485 490 495Pro Val Ile Pro Glu Gly Leu Phe
500<210>603<211>975<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(975)<223>FRXA00406<400>603gac cca gtt gag gtt gct gcg gac act cag gaa atg atc atc acc cgc 48Asp Pro Val Glu Val Ala Ala Asp Thr Gln Glu Met Ile Ile Thr Arg1 5 10 15atc atc gac aat gca tcg gtg cag gca gct tcc gtg ttg cgt cga cca 96Ile Ile Asp Asn Ala Ser Val Gln Ala Ala Ser Val Leu Arg Arg Pro
20 25 30gtt agc tct gcc cgt gcg atg gca cag gtc agg cca gtt acc gat ggt 144Val Ser Ser Ala Arg Ala Met Ala Gln Val Arg Pro Val Thr Asp Gly
35 40 45cgg ggt gca tct gtt ttc ggt ctg cca gga cgt tat gcc gcg gaa tgg 192Arg Gly Ala Ser Val Phe Gly Leu Pro Gly Arg Tyr Ala Ala Glu Trp
50 55 60gct gcg ctt gct aac ggc act gcg gtg cgt gag ctt gat ttc cat gac 240Ala Ala Leu Ala Asn Gly Thr Ala Val Arg Glu Leu Asp Phe His Asp65 70 75 80acg ttc ctc gct gcg gaa tac tcc cac cca gga gat aac att cct ccg 288Thr Phe Leu Ala Ala Glu Tyr Ser His Pro Gly Asp Asn Ile Pro Pro
85 90 95att ttg gct gca gca cag cag gct gga aaa ggt ggc aag gat ctg atc 336Ile Leu Ala Ala Ala Gln Gln Ala Gly Lys Gly Gly Lys Asp Leu Ile
100 105 110cgt ggc atc gct act ggg tat gag att cag gtt aac ttg gtg cgt gga 384Arg Gly Ile Ala Thr Gly Tyr Glu Ile Gln Val Asn Leu Val Arg Gly
115 120 125atg tgc ctg cat gag cac aag att gat cac gtt gct cat ctt gga cca 432Met Cys Leu His Glu His Lys Ile Asp His Val Ala His Leu Gly Pro
130 135 140tca gcg gct gct ggt atc gga acc ttg cta gac cta gat gtg gac acc 480Ser Ala Ala Ala Gly Ile Gly Thr Leu Leu Asp Leu Asp Val Asp Thr145 150 155 160atc tac cag gca att ggt cag gca ttg cac acc acc acg gcg acg agg 528Ile Tyr Gln Ala Ile Gly Gln Ala Leu His Thr Thr Thr Ala Thr Arg
165 170 175cag tcc cgt aaa ggt gcg att tct tca tgg aag gca ttt gct cct gcg 576Gln Ser Arg Lys Gly Ala Ile Ser Ser Trp Lys Ala Phe Ala Pro Ala
180 185 190ttt gcg ggc aag atg tcc atc gag gca gta gat cgc gca atg cgt ggc 624Phe Ala Gly Lys Met Ser Ile Glu Ala Val Asp Arg Ala Met Arg Gly
195 200 205gag ggc gca ccg tca cca atc tgg gaa ggc gaa gac ggc gta atc gcg 672Glu Gly Ala Pro Ser Pro Ile Trp Glu Gly Glu Asp Gly Val Ile Ala
210 215 220tgg ctg ctg tcc ggt ctt gat cac atc tac acc att cct ttg cct gca 720Trp Leu Leu Ser Gly Leu Asp His Ile Tyr Thr Ile Pro Leu Pro Ala225 230 235 240gaa ggt gaa gcc aaa cga gca atc ttg gat acc tac acc aag gaa cac 768Glu Gly Glu Ala Lys Arg Ala Ile Leu Asp Thr Tyr Thr Lys Glu His
245 250 255tcg gcg gaa tac cag tca cag gca ccg atc gac ttg gcg cgc agc atg 816Ser Ala Glu Tyr Gln Ser Gln Ala Pro Ile Asp Leu Ala Arg Ser Met
260 265 270ggg gag aag ctg gca gca cag ggc ttg gac ctg cgt gat gtg gac tcc 864Gly Glu Lys Leu Ala Ala Gln Gly Leu Asp Leu Arg Asp Val Asp Ser
275 280 285atc gtt ttg cac acc tcc cac cac act cac tac gtg atc ggc acc gga 912Ile Val Leu His Thr Ser His His Thr His Tyr Val Ile Gly Thr Gly
290 295 300tct aat gat cca cag aag ttc gat cca gat gca tcg cga gaa acc ctt 960Ser Asn Asp Pro Gln Lys Phe Asp Pro Asp Ala Ser Arg Glu Thr Leu305 310 315 320gat cac tcc atc atg 975Asp His Ser Ile Met
325<210>604<211>325<212>PRT<213>谷氨酸棒杆菌<400>604Asp Pro Val Glu Val Ala Ala Asp Thr Gln Glu Met Ile Ile Thr Arg1 5 10 15Ile Ile Asp Asn Ala Ser Val Gln Ala Ala Ser Val Leu Arg Arg Pro
20 25 30Val Ser Ser Ala Arg Ala Met Ala Gln Val Arg Pro Val Thr Asp Gly
35 40 45Arg Gly Ala Ser Val Phe Gly Leu Pro Gly Arg Tyr Ala Ala Glu Trp
50 55 60Ala Ala Leu Ala Asn Gly Thr Ala Val Arg Glu Leu Asp Phe His Asp65 70 75 80Thr Phe Leu Ala Ala Glu Tyr Ser His Pro Gly Asp Asn Ile Pro Pro
85 90 95Ile Leu Ala Ala Ala Gln Gln Ala Gly Lys Gly Gly Lys Asp Leu Ile
100 105 110Arg Gly Ile Ala Thr Gly Tyr Glu Ile Gln Val Asn Leu Val Arg Gly
115 120 125Met Cys Leu His Glu His Lys Ile Asp His Val Ala His Leu Gly Pro
130 135 140Ser Ala Ala Ala Gly Ile Gly Thr Leu Leu Asp Leu Asp Val Asp Thr145 150 155 160Ile Tyr Gln Ala Ile Gly Gln Ala Leu His Thr Thr Thr Ala Thr Arg
165 170 175Gln Ser Arg Lys Gly Ala Ile Ser Ser Trp Lys Ala Phe Ala Pro Ala
180 185 190Phe Ala Gly Lys Met Ser Ile Glu Ala Val Asp Arg Ala Met Arg Gly
195 200 205Glu Gly Ala Pro Ser Pro Ile Trp Glu Gly Glu Asp Gly Val Ile Ala
210 215 220Trp Leu Leu Ser Gly Leu Asp His Ile Tyr Thr Ile Pro Leu Pro Ala225 230 235 240Glu Gly Glu Ala Lys Arg Ala Ile Leu Asp Thr Tyr Thr Lys Glu His
245 250 255Ser Ala Glu Tyr Gln Ser Gln Ala Pro Ile Asp Leu Ala Arg Ser Met
260 265 270Gly Glu Lys Leu Ala Ala Gln Gly Leu Asp Leu Arg Asp Val Asp Ser
275 280 285Ile Val Leu His Thr Ser His His Thr His Tyr Val Ile Gly Thr Gly
290 295 300Ser Asn Asp Pro Gln Lys Phe Asp Pro Asp Ala Ser Arg Glu Thr Leu305 310 315 320Asp His Ser Ile Met
325<210>605<211>431<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(408)<223>FRXA00514<400>605cac gag cgt tcc tat gct cct gag cga gcc ctc cgc cga gag acc atc 48His Glu Arg Ser Tyr Ala Pro Glu Arg Ala Leu Arg Arg Glu Thr Ile1 5 l0 15gag ctg tgg aac aag att tcc acg gtg gag gat cct gaa tgg acc agg 96Glu Leu Trp Asn Lys Ile Ser Thr Val Glu Asp Pro Glu Trp Thr Arg
20 25 30cgt tac cac tcc gtt gat cct gca gaa aag gcc ttc ggc gca cgc gca 144Arg Tyr His Ser Val Asp Pro Ala Glu Lys Ala Phe Gly Ala Arg Ala
35 40 45gtg atc acc ttc aag gat gga acc gtc gtg gaa gat gaa ctg gct gtg 192Val Ile Thr Phe Lys Asp Gly Thr Val Val Glu Asp Glu Leu Ala Val
50 55 60gcg aat gcg cat cct ctg gga gca cgg cct ttc gct agg gag cag tac 240Ala Asn Ala His Pro Leu Gly Ala Arg Pro Phe Ala Arg Glu Gln Tyr65 70 75 80att cag aaa ttc cgc acc ttg gct gaa ggt gtt gtg tcc gaa aag gaa 288Ile Gln Lys Phe Arg Thr Leu Ala Glu Gly Val Val Ser Glu Lys Glu
85 90 95cag gat cgc ttc ttg gat gcg gca cag cgt acg cac gag ctt gag gat 336Gln Asp Arg Phe Leu Asp Ala Ala Gln Arg Thr His Glu Leu Glu Asp
100 105 110ctt tca gaa ctc aac att gaa ttg gat gcc gat att ttg gcc aag gct 384Leu Ser Glu Leu Asn Ile Glu Leu Asp Ala Asp Ile Leu Ala Lys Ala
115 120 125cct gtg att ccg gaa gga ctg ttc tgatggcggg tttgttttcc tct 431Pro Val Ile Pro Glu Gly Leu Phe
130 135<210>606<211>136<211>136酸棒杆菌<400>606His Glu Arg Ser Tyr Ala Pro Glu Arg Ala Leu Arg Arg Glu Thr Ile1 5 10 15Glu Leu Trp Asn Lys Ile Ser Thr Val Glu Asp Pro Glu Trp Thr Arg
20 25 30Arg Tyr His Ser Val Asp Pro Ala Glu Lys Ala Phe Gly Ala Arg Ala
35 40 45Val Ile Thr Phe Lys Asp Gly Thr Val Val Glu Asp Glu Leu Ala Val
50 55 60Ala Asn Ala His Pro Leu Gly Ala Arg Pro Phe Ala Arg Glu Gln Tyr65 70 75 80Ile Gln Lys Phe Arg Thr Leu Ala Glu Gly Val Val Ser Glu Lys Glu
85 90 95Gln Asp Arg Phe Leu Asp Ala Ala Gln Arg Thr His Glu Leu Glu Asp
100 105 110Leu Ser Glu Leu Asn Ile Glu Leu Asp Ala Asp Ile Leu Ala Lys Ala
115 120 125Pro Val Ile Pro Glu Gly Leu Phe
130 135<210>607<211>718<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(718)<223>RXA00512<400>607tacaacgagt acaacgcttt cgaccagcaa gtattcacct attccgctga cagctacaag 60cccatcttct aacccgccta tatataagga gtgaatcacc atg tcc agc gcc aca 115
Met Ser Ser Ala Thr
1 5acc act gat gtt cgc aaa ggg ctc tac gga gtc atc gcc gat tac acg 163Thr Thr Asp Val Arg Lys Gly Leu Tyr Gly Val Ile Ala Asp Tyr Thr
10 15 20gcc gtt tcc aaa gtc atg cca gag acc aat tca ctg acc tac cgt ggc 211Ala Val Ser Lys Val Met Pro Glu Thr Asn Ser Leu Thr Tyr Arg Gly
25 30 35tac gcg gtg gaa gat ttg gtg gaa aac tgc agc ttc gag gag gtg ttt 259Tyr Ala Val Glu Asp Leu Val Glu Asn Cys Ser Phe Glu Glu Val Phe
40 45 50tac ctc ctg tgg cac ggc gag ctg ccc act gcg caa caa ctt gcg gag 307Tyr Leu Leu Trp His Gly Glu Leu Pro Thr Ala Gln Gln Leu Ala Glu
55 60 65ttc aat gag cgt ggc cgt tcc tac cgc tcc ctg gat gcc ggt ttg atc 355Phe Asn Glu Arg Gly Arg Ser Tyr Arg Ser Leu Asp Ala Gly Leu Ile70 75 80 85tcc ctg atc cac tct ttg ccc aaa gaa gcc cac ccg atg gat gtt atg 403Ser Leu Ile His Ser Leu Pro Lys Glu Ala His Pro Met Asp Val Met
90 95 100cgc acc gcg gtg tcc tac atg ggc acc aag gat tcc gag tat ttc acc 451Arg Thr Ala Val Ser Tyr Met Gly Thr Lys Asp Ser Glu Tyr Phe Thr
105 110 115acc gat tct gag cac atc cgc aaa gtt ggc cac acc ttg ttg gcg cag 499Thr Asp Ser Glu His Ile Arg Lys Val Gly His Thr Leu Leu Ala Gln
120 125 130ctt ccg atg gtg cta gcc atg gat att cgt cgc cgc aag ggc ctc gat 547Leu Pro Met Val Leu Ala Met Asp Ile Arg Arg Arg Lys Gly Leu Asp
135 140 145atc atc gcc cct gac tcc agc aag tca gtc gcc gaa aac ctg ctg tct 595Ile Ile Ala Pro Asp Ser Ser Lys Ser Val Ala Glu Asn Leu Leu Ser150 155 160 165atg gtg ttt ggt act ggc ccg gaa tca cct gca tcc aac cca gct gac 643Met Val Phe Gly Thr Gly Pro Glu Ser Pro Ala Ser Asn Pro Ala Asp
170 175 180gtc cgc gat ttt gag aaa tca ctg atc ctc tac gcc gag cac tcc ttc 691Val Arg Asp Phe Glu Lys Ser Leu Ile Leu Tyr Ala Glu His Ser Phe
185 190 195aac gcc tcc acc ttc acc gcc cgc gtg 718Asn Ala Ser Thr Phe Thr Ala Arg Val
200 205<210>608<211>206<212>PRT<213>谷氨酸棒杆菌<400>608Met Ser Ser Ala Thr Thr Thr Asp Val Arg Lys Gly Leu Tyr Gly Val1 5 10 15Ile Ala Asp Tyr Thr Ala Val Ser Lys Val Met Pro Glu Thr Asn Ser
20 25 30Leu Thr Tyr Arg Gly Tyr Ala Val Glu Asp Leu Val Glu Asn Cys Ser
35 40 45Phe Glu Glu Val Phe Tyr Leu Leu Trp His Gly Glu Leu Pro Thr Ala
50 55 60Gln Gln Leu Ala Glu Phe Asn Glu Arg Gly Arg Ser Tyr Arg Ser Leu65 70 75 80Asp Ala Gly Leu Ile Ser Leu Ile His Ser Leu Pro Lys Glu Ala His
85 90 95Pro Met Asp Val Met Arg Thr Ala Val Ser Tyr Met Gly Thr Lys Asp
100 105 110Ser Glu Tyr Phe Thr Thr Asp Ser Glu His Ile Arg Lys Val Gly His
115 120 125Thr Leu Leu Ala Gln Leu Pro Met Val Leu Ala Met Asp Ile Arg Arg
130 135 140Arg Lys Gly Leu Asp Ile Ile Ala Pro Asp Ser Ser Lys Ser Val Ala145 150 155 160Glu Asn Leu Leu Ser Met Val Phe Gly Thr Gly Pro Glu Ser Pro Ala
165 170 175Ser Asn Pro Ala Asp Val Arg Asp Phe Glu Lys Ser Leu Ile Leu Tyr
180 185 190Ala Glu His Ser Phe Asn Ala Ser Thr Phe Thr Ala Arg Val
195 200 205<210>609<211>320<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(297)<223>RXA00518<400>609gtc ctt ccg gta tta gct gcc cgc cac gac ggc gaa aag tgg gtt gcc 48Val Leu Pro Val Leu Ala Ala Arg His Asp Gly Glu Lys Trp Val Ala1 5 10 15atg tat gaa aac atg cgc gac gcc atg gac gcc cgc acc ggc atc aag 96Met Tyr Glu Asn Met Arg Asp Ala Met Asp Ala Arg Thr Gly Ile Lys
20 25 30ccg aat ctc gat ttc cct gct ggc cct gcc tac cac ctg ctc ggt ttc 144Pro Asn Leu Asp Phe Pro Ala Gly Pro Ala Tyr His Leu Leu Gly Phe
35 40 45ccg gtc gat ttc ttc acc ccg ctg ttc gtc atc gcc cgc gtc gcc ggc 192Pro Val Asp Phe Phe Thr Pro Leu Phe Val Ile Ala Arg Val Ala Gly
50 55 60tgg acg gcc cac atc gtg gag cag tac gaa aac aac tcg ctc atc cgc 240Trp Thr Ala His Ile Val Glu Gln Tyr Glu Asn Asn Ser Leu Ile Arg65 70 75 80cca ctg tcc gag tac aac ggc gag gag cag cgc gag gtc gcg ccc att 288Pro Leu Ser Glu Tyr Asn Gly Glu Glu Gln Arg Glu Val Ala Pro Ile
85 90 95gaa aag cgc taaaagattt tcgcttttcg acg 320Glu Lys Arg<210>610<211>99<212>PRT<213>谷氨酸棒杆菌<400>610Val Leu Pro Val Leu Ala Ala Arg His Asp Gly Glu Lys Trp Val Ala1 5 10 15Met Tyr Glu Asn Met Arg Asp Ala Met Asp Ala Arg Thr Gly Ile Lys
20 25 30Pro Asn Leu Asp Phe Pro Ala Gly Pro Ala Tyr His Leu Leu Gly Phe
35 40 45Pro Val Asp Phe Phe Thr Pro Leu Phe Val Ile Ala Arg Val Ala Gly
50 55 60Trp Thr Ala His Ile Val Glu Gln Tyr Glu Asn Asn Ser Leu Ile Arg65 70 75 80Pro Leu Ser Glu Tyr Asn Gly Glu Glu Gln Arg Glu Val Ala Pro Ile
85 90 95Glu Lys Arg<210>611<211>1494<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1471)<223>RXA01077<400>611tctatgactt gatccacaat gtgatgcaaa tcattgaccc tcaccccgga ccaagcgctt 60aatgaaggca agccaaactt aactagtaga taggattgca atg acc gaa tcg caa 115
Met Thr Glu Ser Gln
1 5gat ctc gcc gca ttc gtg gaa gct gcc aaa ctc aat gat gca agc ccc 163Asp Leu Ala Ala Phe Val Glu Ala Ala Lys Leu Asn Asp Ala Ser Pro
10 15 20gaa gcc gta gag caa ttg aaa atc aga gtg cta gac acc gta ggc gtt 211Glu Ala Val Glu Gln Leu Lys Ile Arg Val Leu Asp Thr Val Gly Val
25 30 35gcc att ggc gca ctg gat gcc gaa ccg att gtc gcc att cga gga ctc 259Ala Ile Gly Ala Leu Asp Ala Glu Pro Ile Val Ala Ile Arg Gly Leu
40 45 50ctg gaa gac ctc ggg gga acc gaa cag tca aca ctt att ggt ggt ggc 307Leu Glu Asp Leu Gly Gly Thr Glu Gln Ser Thr Leu Ile Gly Gly Gly
55 60 65aaa acc agt ccg gaa cgt gca gca ttt ttc aac agc gca tta agc cgc 355Lys Thr Ser Pro Glu Arg Ala Ala Phe Phe Asn Ser Ala Leu Ser Arg70 75 80 85tac ctc gac ttc atg gac gcc tac cta gca aag ggc gaa acc aac cac 403Tyr Leu Asp Phe Met Asp Ala Tyr Leu Ala Lys Gly Glu Thr Asn His
90 95 100ccc tcg gat aac ttc gga gca gtg ctc gct gca gcc gaa agc gtt ggc 451Pro Ser Asp Asn Phe Gly Ala Val Leu Ala Ala Ala Glu Ser Val Gly
105 110 115gcc tct gga aaa gac ctg ctc acc gca ttc gcc gtg gcc tac cag gta 499Ala Ser Gly Lys Asp Leu Leu Thr Ala Phe Ala Val Ala Tyr Gln Val
120 125 130cac acc aga ctt tca gat gtc gca cca gtt cgc gcc aaa ggt ttc gat 547His Thr Arg Leu Ser Asp Val Ala Pro Val Arg Ala Lys Gly Phe Asp
135 140 145cac acc acc caa gga gca ttc gca gcg ggc gca tct gct gcc aag gca 595His Thr Thr Gln Gly Ala Phe Ala Ala Gly Ala Ser Ala Ala Lys Ala150 155 160 165ctg ggt ttg cca gct gat caa atc gcc aac gca ctg gcc atc gca gga 643Leu Gly Leu Pro Ala Asp Gln Ile Ala Asn Ala Leu Ala Ile Ala Gly
170 175 180aca gcc aat gtt gca ctt cgt gtc act cgc act gga aac ttg agc cac 691Thr Ala Asn Val Ala Leu Arg Val Thr Arg Thr Gly Asn Leu Ser His
185 190 195tgg aaa ggc ctt gcc tac cca cac gtg tcc aaa gaa gga acc tgg gca 739Trp Lys Gly Leu Ala Tyr Pro His Val Ser Lys Glu Gly Thr Trp Ala
200 205 210gca ctg ctc gca agc cga ggt att acc ggt ccg gaa gaa gtc ttc gaa 787Ala Leu Leu Ala Ser Arg Gly Ile Thr Gly Pro Glu Glu Val Phe Glu
215 220 225ggc aac aag gga ttc aaa gag tcc gtc tcc gga ccg ttc gag atc gat 835Gly Asn Lys Gly Phe Lys Glu Ser Val Ser Gly Pro Phe Glu Ile Asp230 235 240 245tgg tcc aag gaa gac ttg gaa agc gtt aag cgc acc atc atc aag aaa 883Trp Ser Lys Glu Asp Leu Glu Ser Val Lys Arg Thr Ile Ile Lys Lys
250 255 260cac aac gcg gaa att cac tcg cag tca gcg ctt gat gca gcc caa gaa 931His Asn Ala Glu Ile His Ser Gln Ser Ala Leu Asp Ala Ala Gln Glu
265 270 275ata cgc gca caa gaa ggc ttc aat gtg gac aac att gaa aag att cac 979Ile Arg Ala Gln Glu Gly Phe Asn Val Asp Asn Ile Glu Lys Ile His
280 285 290ctg act act ttc gac gtt gcc tac tcc atc att ggc ggc ggc gaa gaa 1027Leu Thr Thr Phe Asp Val Ala Tyr Ser Ile Ile Gly Gly Gly Glu Glu
295 300 305ggc gac aaa cag ctt att cgc acc aaa gaa gaa gcc gat cac tca ctg 1075Gly Asp Lys Gln Leu Ile Arg Thr Lys Glu Glu Ala Asp His Ser Leu310 315 320 325ccg tgg atg ctc gct gta gtt ctg ctg gat ggt cag ctc aat ccc gaa 1123Pro Trp Met Leu Ala Val Val Leu Leu Asp Gly Gln Leu Asn Pro Glu
330 335 340cag tac gaa cca tca cgc atc gtt gct gat gat gta caa acc ttg atg 1171Gln Tyr Glu Pro Ser Arg Ile Val Ala Asp Asp Val Gln Thr Leu Met
345 350 355aag aaa atc gaa atc aca ccg tca gat gaa ttc tct gat cgc ttc cct 1219Lys Lys Ile Glu Ile Thr Pro Ser Asp Glu Phe Ser Asp Arg Phe Pro
360 365 370gac cac atg cca gct gat cta gaa gtc aca cta aac gat ggc tcg gtg 1267Asp His Met Pro Ala Asp Leu Glu Val Thr Leu Asn Asp Gly Ser Val
375 380 385ttc aaa gct tca caa gat agc tac tta ggc ttc cac gac aat ccc cta 1315Phe Lys Ala Ser Gln Asp Ser Tyr Leu Gly Phe His Asp Asn Pro Leu390 395 400 405gat tgg gac aac gcg cgc aag aaa ttc gat gcc ctt gtc aca cca ttc 1363Asp Trp Asp Asn Ala Arg Lys Lys Phe Asp Ala Leu Val Thr Pro Phe
410 415 420acc ggt gaa gaa cta cgt gaa gaa atc gcc acg atc att cac gag ctc 1411Thr Gly Glu Glu Leu Arg Glu Glu Ile Ala Thr Ile Ile His Glu Leu
425 430 435gat agc cga cag gtt tct gaa ctc aca gaa gcc ctg gcc aaa gtc tcc 1459Asp Ser Arg Gln Val Ser Glu Leu Thr Glu Ala Leu Ala Lys Val Ser
440 445 450acc acc cgc agc taaaactttt tgaaaggagc tca 1494Thr Thr Arg Ser
455<210>612<211>457<212>PRT<213>谷氨酸棒杆菌<400>612Met Thr Glu Ser Gln Asp Leu Ala Ala Phe Val Glu Ala Ala Lys Leu1 5 10 15Asn Asp Ala Ser Pro Glu Ala Val Glu Gln Leu Lys Ile Arg Val Leu
20 25 30Asp Thr Val Gly Val Ala Ile Gly Ala Leu Asp Ala Glu Pro Ile Val
35 40 45Ala Ile Arg Gly Leu Leu Glu Asp Leu Gly Gly Thr Glu Gln Ser Thr
50 55 60Leu Ile Gly Gly Gly Lys Thr Ser Pro Glu Arg Ala Ala Phe Phe Asn65 70 75 80Ser Ala Leu Ser Arg Tyr Leu Asp Phe Met Asp Ala Tyr Leu Ala Lys
85 90 95Gly Glu Thr Asn His Pro Ser Asp Asn Phe Gly Ala Val Leu Ala Ala
100 105 110Ala Glu Ser Val Gly Ala Ser Gly Lys Asp Leu Leu Thr Ala Phe Ala
115 120 125Val Ala Tyr Gln Val His Thr Arg Leu Ser Asp Val Ala Pro Val Arg
130 135 140Ala Lys Gly Phe Asp His Thr Thr Gln Gly Ala Phe Ala Ala Gly Ala145 150 155 160Ser Ala Ala Lys Ala Leu Gly Leu Pro Ala Asp Gln Ile Ala Asn Ala
165 170 175Leu Ala Ile Ala Gly Thr Ala Asn Val Ala Leu Arg Val Thr Arg Thr
180 185 190Gly Asn Leu Ser His Trp Lys Gly Leu Ala Tyr Pro His Val Ser Lys
195 200 205Glu Gly Thr Trp Ala Ala Leu Leu Ala Ser Arg Gly Ile Thr Gly Pro
210 215 220Glu Glu Val Phe Glu Gly Asn Lys Gly Phe Lys Glu Ser Val Ser Gly225 230 235 240Pro Phe Glu Ile Asp Trp Ser Lys Glu Asp Leu Glu Ser Val Lys Arg
245 250 255Thr Ile Ile Lys Lys His Asn Ala Glu Ile His Ser Gln Ser Ala Leu
260 265 270Asp Ala Ala Gln Glu Ile Arg Ala Gln Glu Gly Phe Asn Val Asp Asn
275 280 285Ile Glu Lys Ile His Leu Thr Thr Phe Asp Val Ala Tyr Ser Ile Ile
290 295 300Gly Gly Gly Glu Glu Gly Asp Lys Gln Leu Ile Arg Thr Lys Glu Glu305 310 315 320Ala Asp His Ser Leu Pro Trp Met Leu Ala Val Val Leu Leu Asp Gly
325 330 335Gln Leu Asn Pro Glu Gln Tyr Glu Pro Ser Arg Ile Val Ala Asp Asp
340 345 350Val Gln Thr Leu Met Lys Lys Ile Glu Ile Thr Pro Ser Asp Glu Phe
355 360 365Ser Asp Arg Phe Pro Asp His Met Pro Ala Asp Leu Glu Val Thr Leu
370 375 380Asn Asp Gly Ser Val Phe Lys Ala Ser Gln Asp Ser Tyr Leu Gly Phe385 390 395 400His Asp Asn Pro Leu Asp Trp Asp Asn Ala Arg Lys Lys Phe Asp Ala
405 410 415Leu Val Thr Pro Phe Thr Gly Glu Glu Leu Arg Glu Glu Ile Ala Thr
420 425 430Ile Ile His Glu Leu Asp Ser Arg Gln Val Ser Glu Leu Thr Glu Ala
435 440 445Leu Ala Lys Val Ser Thr Thr Arg Ser
450 455<210>613<211>923<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(900)<223>RXN03144<400>613acc acg acg gct acc agg cag tcg cga aaa ggt gag att tcc agc tgg 48Thr Thr Thr Ala Thr Arg Gln Ser Arg Lys Gly Glu Ile Ser Ser Trp1 5 10 15aag gcg ttc gcg cca gcg ttt gcg gga aag atg gcc att gag gcg atg 96Lys Ala Phe Ala Pro Ala Phe Ala Gly Lys Met Ala Ile Glu Ala Met
20 25 30gat cgt gcg atg cgt ggg gag ggt tcg ccc gca ccg att tgg gag ggc 144Asp Arg Ala Met Arg Gly Glu Gly Ser Pro Ala Pro Ile Trp Glu Gly
35 40 45gaa gac ggg gtc atc gcg tgg ctg tta tcg ggc aaa gat cat gtt tat 192Glu Asp Gly Val Ile Ala Trp Leu Leu Ser Gly Lys Asp His Val Tyr
50 55 60cat gtg cca ttg ccg gaa cac ggc gag ccc aag ctg ggg att cta gag 240His Val Pro Leu Pro Glu His Gly Glu Pro Lys Leu Gly Ile Leu Glu65 70 75 80act tac aca aag gaa cat tca gcg gaa tat caa tcg cag gca ccg att 288Thr Tyr Thr Lys Glu His Ser Ala Glu Tyr Gln Ser Gln Ala Pro Ile
85 90 95gat ctg gcg cgc agg atg aag cca ctg gtt gac gcg gct ggc gga acg 336Asp Leu Ala Arg Arg Met Lys Pro Leu Val Asp Ala Ala Gly Gly Thr
100 105 110gaa cac att gca gag att gtg ctg cgc acc agt cac cac acg cat tat 384Glu His Ile Ala Glu Ile Val Leu Arg Thr Ser His His Thr His Tyr
115 120 125gtg att ggc act ggg gcg aac gat ccg cag aag atg gat ccg cag gcc 432Val Ile Gly Thr Gly Ala Asn Asp Pro Gln Lys Met Asp Pro Gln Ala
130 135 140tcg cgt gaa acc ctg gat cat tcc atc atg tac att ttc gcc gtc gcg 480Ser Arg Glu Thr Leu Asp His Ser Ile Met Tyr Ile Phe Ala Val Ala145 150 155 160ctt caa gat ggc gtg tgg cac cac gag ttt tcc tac acc cgc aag cgt 528Leu Gln Asp Gly Val Trp His His Glu Phe Ser Tyr Thr Arg Lys Arg
165 170 175tcc acc cgc ccg gaa act gtg gag ctg tgg cac aag att cgc acc gtg 576Ser Thr Arg Pro Glu Thr Val Glu Leu Trp His Lys Ile Arg Thr Val
180 185 190gag gat cct gaa tgg acg cgc cga tac cat tct gat gat cct gca aaa 624Glu Asp Pro Glu Trp Thr Arg Arg Tyr His Ser Asp Asp Pro Ala Lys
195 200 205aag gcc ttt ggt gcg aaa gca gtg atc aca atg gct gat ggc acc gtg 672Lys Ala Phe Gly Ala Lys Ala Val Ile Thr Met Ala Asp Gly Thr Val
210 215 220att gag gat gaa ttg gct gtc gcg gat gcc cac ccg ctg ggt gct cgg 720Ile Glu Asp Glu Leu Ala Val Ala Asp Ala His Pro Leu Gly Ala Arg225 230 235 240ccg ttt gcg cgg gag aat tac att gaa aaa ttc cgc aca ctc gcg cag 768Pro Phe Ala Arg Glu Asn Tyr Ile Glu Lys Phe Arg Thr Leu Ala Gln
245 250 255ggg att gtc att gat tca gaa cag gaa cgc ttc ttg cat gcc gtg caa 816Gly Ile Val Ile Asp Ser Glu Gln Glu Arg Phe Leu His Ala Val Gln
260 265 270agc ctg cct gac ctg gat gat ctt gat cag ctc aac atc gaa gtc gac 864Ser Leu Pro Asp Leu Asp Asp Leu Asp Gln Leu Asn Ile Glu Val Asp
275 280 285ata agc aac cag gcc gcg acg aaa gcg ggg ctg tta tgaatctctt 910Ile Ser Asn Gln Ala Ala Thr Lys Ala Gly Leu Leu
290 295 300ttcgaatggt gtt 923<210>614<211>300<212>PRT<213>谷氨酸棒杆菌<400>614Thr Thr Thr Ala Thr Arg Gln Ser Arg Lys Gly Glu Ile Ser Ser Trp1 5 10 15Lys Ala Phe Ala Pro Ala Phe Ala Gly Lys Met Ala Ile Glu Ala Met
20 25 30Asp Arg Ala Met Arg Gly Glu Gly Ser Pro Ala Pro Ile Trp Glu Gly
35 40 45Glu Asp Gly Val Ile Ala Trp Leu Leu Ser Gly Lys Asp His Val Tyr
50 55 60His Val Pro Leu Pro Glu His Gly Glu Pro Lys Leu Gly Ile Leu Glu65 70 75 80Thr Tyr Thr Lys Glu His Ser Ala Glu Tyr Gln Ser Gln Ala Pro Ile
85 90 95Asp Leu Ala Arg Arg Met Lys Pro Leu Val Asp Ala Ala Gly Gly Thr
100 105 110Glu His Ile Ala Glu Ile Val Leu Arg Thr Ser His His Thr His Tyr
115 120 125Val Ile Gly Thr Gly Ala Asn Asp Pro Gln Lys Met Asp Pro Gln Ala
130 135 140Ser Arg Glu Thr Leu Asp His Ser Ile Met Tyr Ile Phe Ala Val Ala145 150 155 160Leu Gln Asp Gly Val Trp His His Glu Phe Ser Tyr Thr Arg Lys Arg
165 170 175Ser Thr Arg Pro Glu Thr Val Glu Leu Trp His Lys Ile Arg Thr Val
180 185 190Glu Asp Pro Glu Trp Thr Arg Arg Tyr His Ser Asp Asp Pro Ala Lys
195 200 205Lys Ala Phe Gly Ala Lys Ala Val Ile Thr Met Ala Asp Gly Thr Val
210 215 220Ile Glu Asp Glu Leu Ala Val Ala Asp Ala His Pro Leu Gly Ala Arg225 230 235 240Pro Phe Ala Arg Glu Asn Tyr Ile Glu Lys Phe Arg Thr Leu Ala Gln
245 250 255Gly Ile Val Ile Asp Ser Glu Gln Glu Arg Phe Leu His Ala Val Gln
260 265 270Ser Leu Pro Asp Leu Asp Asp Leu Asp Gln Leu Asn Ile Glu Val Asp
275 280 285Ile Ser Asn Gln Ala Ala Thr Lys Ala Gly Leu Leu
290 295 300<210>615<211>511<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(511)<223>FRXA02322<400>615cgcgcagagc taaactgcgt gaggttgtgg cctgtcacac ataatcggcc tagggtggga 60ctttaaggaa acagtgcaca aataaatctc aaggagcccc atg cgc atc cac gat 115
Met Arg Ile His Asp
1 5gtt tat acc cac ctt tcg gcc gat aac ttt ccc aaa gca gag cac ctt 163Val Tyr Thr His Leu Ser Ala Asp Asn Phe Pro Lys Ala Glu His Leu
10 15 20gcg tgg aaa ttc tcc gag ctt gcc acc gac ccc gtg gag gtg aca ccg 211Ala Trp Lys Phe Ser Glu Leu Ala Thr Asp Pro Val Glu Val Thr Pro
25 30 35gat gtt tcg gag atg atc atc aac cgg atc atc gac aac gcg gcg gtg 259Asp Val Ser Glu Met Ile Ile Asn Arg Ile Ile Asp Asn Ala Ala Val
40 45 50tct gcc gcg tcg gtg ttg cgc cgg cct gtg act gtg gcc agg caa caa 307Ser Ala Ala Ser Val Leu Arg Arg Pro Val Thr Val Ala Arg Gln Gln
55 60 65gcg cag tcc cat ccg cgg gaa aag ggc gga aaa gtt ttt gga att tca 355Ala Gln Ser His Pro Arg Glu Lys Gly Gly Lys Val Phe Gly Ile Ser70 75 80 85ggc agc tac rca cca gag tgg gct gcc ttt gct aat ggt gtg gcc gta 403Gly Ser Tyr Ser Pro Glu Trp Ala Ala Phe Ala Asn Gly Val Ala Val
90 95 100cgt gaa ttg gac ttc cac gat aca ttt tta gca gct gaa tac tcc cat 451Arg Glu Leu Asp Phe His Asp Thr Phe Leu Ala Ala GLu Tyr Ser His
105 110 115ccc ggc gac aat att cca cca ctt ctt gca gta gcg cag gct cag aga 499Pro Gly Asp Asn Ile Pro Pro Leu Leu Ala Val Ala Gln Ala Gln Arg
120 125 130agc agc ggc agg 511Ser Ser Gly Arg
135<210>616<211>137<212>PRT<213>谷氨酸棒杆菌<400>616Met Arg Ile His Asp Val Tyr Thr His Leu Ser Ala Asp Asn Phe Pro1 5 10 15Lys Ala Glu His Leu Ala Trp Lys Phe Ser Glu Leu Ala Thr Asp Pro
20 25 30Val Glu Val Thr Pro Asp Val Ser Glu Met Ile Ile Asn Arg Ile Ile
35 40 45Asp Asn Ala Ala Val Ser Ala Ala Ser Val Leu Arg Arg Pro Val Thr
50 55 60Val Ala Arg Gln Gln Ala Gln Ser His Pro Arg Glu Lys Gly Gly Lys65 70 75 80Val Phe Gly Ile Ser Gly Ser Tyr Ser Pro Glu Trp Ala Ala Phe Ala
85 90 95Asn Gly Val Ala Val Arg Glu Leu Asp Phe His Asp Thr Phe Leu Ala
100 105 110Ala Glu Tyr Ser His Pro Gly Asp Asn Ile Pro Pro Leu Leu Ala Val
115 120 125Ala Gln Ala Gln Arg Ser Ser Gly Arg
130 135<210>617<211>603<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(603)<223>RXA02329<400>617acg gct acc agg cag tcg cga aaa ggt gag att tcc agc tgg aag gcg 48Thr Ala Thr Arg Gln Ser Arg Lys Gly Glu Ile Ser Ser Trp Lys Ala1 5 10 15ttc gcg cca gcg ttt gcg gga aag atg gcc att gag gcg atg gat cgt 96Phe Ala Pro Ala Phe Ala Gly Lys Met Ala Ile Glu Ala Met Asp Arg
20 25 30gcg atg cgt ggg gag ggt tcg ccc gca ccg att tgg gag ggc gaa gac 144Ala Met Arg Gly Glu Gly Ser Pro Ala Pro Ile Trp Glu Gly Glu Asp
35 40 45ggg gtc atc gcg tgg ctg tta tcg ggc aaa gat cat gtt tat cat gtg 192Gly Val Ile Ala Trp Leu Leu Ser Gly Lys Asp His Val Tyr His Val
50 55 60cca ttg ccg gaa cac ggc gag ccc aag ctg ggg att cta gag act tac 240Pro Leu Pro Glu His Gly Glu Pro Lys Leu Gly Ile Leu Glu Thr Tyr65 70 75 80aca aag gaa cat tca gcg gaa tat caa tcg cag gca ccg att gat ctg 288Thr Lys Glu His Ser Ala Glu Tyr Gln Ser Gln Ala Pro Ile Asp Leu
85 90 95gcg cgc agg atg aag cca ctg gtt gac gcg gct ggc gga acg gaa cac 336Ala Arg Arg Met Lys Pro Leu Val Asp Ala Ala Gly Gly Thr Glu His
100 105 110att gca gag att gtg ctg cgc acc agt cac cac acg cat tat gtg att 384Ile Ala Glu Ile Val Leu Arg Thr Ser His His Thr His Tyr Val Ile
115 120 125ggc act ggg gcg aac gat ccg cag aag atg gat ccg cag gcc tcg cgt 432Gly Thr Gly Ala Asn Asp Pro Gln Lys Met Asp Pro Gln Ala Ser Arg
130 135 140gaa acc ctg gat cat tcc atc atg tac att ttc gcc gtc gcg ctt caa 480Glu Thr Leu Asp His Ser Ile Met Tyr Ile Phe Ala Val Ala Leu Gln145 150 155 160gat ggc gtg tgg cac cac gag ttt tcc tac acc cgc aag cgt tcc acc 528Asp Gly Val Trp His His Glu Phe Ser Tyr Thr Arg Lys Arg Ser Thr
165 170 175cgc ccg gaa act gtg gag ctg tgg cac aag att cgc acc gtg gag gat 576Arg Pro Glu Thr Val Glu Leu Trp His Lys Ile Arg Thr Val Glu Asp
180 185 190cct gaa tgg acg cgc cga tac cat tct 603Pro Glu Trp Thr Arg Arg Tyr His Ser
195 200<210>618<211>201<212>PRT<213>谷氨酸棒杆菌<400>618Thr Ala Thr Arg Gln Ser Arg Lys Gly Glu Ile Ser Ser Trp Lys Ala1 5 10 15Phe Ala Pro Ala Phe Ala Gly Lys Met Ala Ile Glu Ala Met Asp Arg
20 25 30Ala Met Arg Gly Glu Gly Ser Pro Ala Pro Ile Trp Glu Gly Glu Asp
35 40 45Gly Val Ile Ala Trp Leu Leu Ser Gly Lys Asp His Val Tyr His Val
50 55 60Pro Leu Pro Glu His Gly Glu Pro Lys Leu Gly Ile Leu Glu Thr Tyr65 70 75 80Thr Lys Glu His Ser Ala Glu Tyr Gln Ser Gln Ala Pro Ile Asp Leu
85 90 95Ala Arg Arg Met Lys Pro Leu Val Asp Ala Ala Gly Gly Thr Glu His
100 105 110Ile Ala Glu Ile Val Leu Arg Thr Ser His His Thr His Tyr Val Ile
115 120 125Gly Thr Gly Ala Asn Asp Pro Gln Lys Met Asp Pro Gln Ala Ser Arg
130 135 140Glu Thr Leu Asp His Ser Ile Met Tyr Ile Phe Ala Val Ala Leu Gln145 150 155 160Asp Gly Val Trp His His Glu Phe Ser Tyr Thr Arg Lys Arg Ser Thr
165 170 175Arg Pro Glu Thr Val Glu Leu Trp His Lys Ile Arg Thr Val Glu Asp
180 185 190Pro Glu Trp Thr Arg Arg Tyr His Ser
195 200<210>619<211>1266<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1243)<223>RXA02332<400>619gaccgcatgc aacaccgcag cagattatat gagctcctgc gatacgaaga ctacaacgtc 60tttgaccagc acattttcac ctacagaaaa ggagaaaaca atg agt gac agc caa 115
Met Ser Asp Ser Gln
1 5gtc cgc aaa gga ctc aac ggc gtc atc tct gac tac aca agc att tcc 163Val Arg Lys Gly Leu Asn Gly Val Ile Ser Asp Tyr Thr Ser Ile Ser
10 15 20aaa gtg atg cca gag agc aac tcg ctg act tac cgt ggc tac gcc gtg 211Lys Val Met Pro Glu Ser Asn Ser Leu Thr Tyr Arg Gly Tyr Ala Val
25 30 35gag gat ttg gtg gaa aac tgc agc ttt gaa gaa gtg atc tac ctc ctg 259Glu Asp Leu Val Glu Asn Cys Ser Phe Glu Glu Val Ile Tyr Leu Leu
40 45 50tgg ttt ggg gag ctg ccc acc act gaa caa ctc cgg acc ttc aac acg 307Trp Phe Gly Glu Leu Pro Thr Thr Glu Gln Leu Arg Thr Phe Asn Thr
55 60 65aca ggt cga agc tac cgc tca ctc gac gcc gga ctg att tcc ctc atc 355Thr Gly Arg Ser Tyr Arg Ser Leu Asp Ala Gly Leu Ile Ser Leu Ile70 75 80 85cac tcc tta ccc aac acc tgc cac ccc atg gac gtg ctg cgc acc gca 403His Ser Leu Pro Asn Thr Cys His Pro Met Asp Val Leu Arg Thr Ala
90 95 100gtg tcc tac atg ggt acc ttt gat ccc gat ccg ttt acc cgc gat gcc 451Val Ser Tyr Met Gly Thr Phe Asp Pro Asp Pro Phe Thr Arg Asp Ala
105 110 115gat cat atc cga agc att gga cac aac ctg ctt gcg cag ctt ccc atg 499Asp His Ile Arg Ser Ile Gly His Asn Leu Leu Ala Gln Leu Pro Met
120 125 130gtg gtt gcc atg gat atc cgc agg cga agt ggg gaa gag atc atc gca 547Val Val Ala Met Asp Ile Arg Arg Arg Ser Gly Glu Glu Ile Ile Ala
135 140 145cct gac cac aac aaa ggt atc gct tcg aat ttc tta tcc atg gtg ttt 595Pro Asp His Asn Lys Gly Ile Ala Ser Asn Phe Leu Ser Met Val Phe150 155 160 165ggc aat gat gat ggt tct gta gcc aac tcc gca gat gac atc cgc gat 643Gly Asn Asp Asp Gly Ser Val Ala Asn Ser Ala Asp Asp Ile Arg Asp
170 175 180ttt gaa cgc tcc ctc atc ctc tac gcc gag cac tcc ttc aac gcc tcc 691Phe Glu Arg Ser Leu Ile Leu Tyr Ala Glu His Ser Phe Asn Ala Ser
185 190 195aca ttc tca gcc cgc gtg atc tca tca acg cga tcc gat acg tat tcg 739Thr Phe Ser Ala Arg Val Ile Ser Ser Thr Arg Ser Asp Thr Tyr Ser
200 205 210gcg atc aca ggt gcg atc ggt gct ctc aaa ggc cca ctg cac gga ggt 787Ala Ile Thr Gly Ala Ile Gly Ala Leu Lys Gly Pro Leu His Gly Gly
215 220 225gcc aat gag ttt gtc atg cac acc atg ctg gat atc gac gat ccc aac 835Ala Asn Glu Phe Val Met His Thr Met Leu Asp Ile Asp Asp Pro Asn230 235 240 245aat gct gcc gac tgg atg ggc aag gcg ttg gat cgt aaa gaa cgc atc 883Asn Ala Ala Asp Trp Met Gly Lys Ala Leu Asp Arg Lys Glu Arg Ile
250 255 260atg gga ttc ggg cac cgc gtg tac aaa aac ggc gac tcc agg gtc ccc 931Met Gly Phe Gly His Arg Val Tyr Lys Asn Gly Asp Ser Arg Val Pro
265 270 275tcc atg gag aaa tcc atg cgc tcc ctt gct gct cgt cac cgt ggt caa 979Ser Met Glu Lys Ser Met Arg Ser Leu Ala Ala Arg His Arg Gly Gln
280 285 290aaa tgg gtg cac atg tat gag tcg atg caa gaa gtc atg gag gct cgc 1027Lys Trp Val His Met Tyr Glu Ser Met Gln Glu Val Met Glu Ala Arg
295 300 305act ggc att aaa ccc aac ctc gac ttc ccg gcc ggc cct gcc tat tac 1075Thr Gly Ile Lys Pro Asn Leu Asp Phe Pro Ala Gly Pro Ala Tyr Tyr310 315 320 325atg ctg gga ttc ccc gtc gac ttc ttc aca cca ctg ttt gtg ctg gcc 1123Met Leu Gly Phe Pro Val Asp Phe Phe Thr Pro Leu Phe Val Leu Ala
330 335 340cga gtg tca ggg tgg acg gca cac atc gtg gag caa ttt gaa aac aat 1171Arg Val Ser Gly Trp Thr Ala His Ile Val Glu Gln Phe Glu Asn Asn
345 350 355gcg ctg atc cga cca ttg tct gcc tac aac gga gtg gaa gaa agg gag 1219Ala Leu Ile Arg Pro Leu Ser Ala Tyr Asn Gly Val Glu Glu Arg Glu
360 365 370gtg gtg ccc att tcg gag aga acc taatcagtga ggctgatttc taa 1266Val Val Pro Ile Ser Glu Arg Thr
375 380<210>620<211>381<212>PRT<213>谷氨酸棒杆菌<400>620Met Ser Asp Ser Gln Val Arg Lys Gly Leu Asn Gly Val Ile Ser Asp1 5 10 15Tyr Thr Ser Ile Ser Lys Val Met Pro Glu Ser Asn Ser Leu Thr Tyr
20 25 30Arg Gly Tyr Ala Val Glu Asp Leu Val Glu Asn Cys Ser Phe Glu Glu
35 40 45Val Ile Tyr Leu Leu Trp Phe Gly Glu Leu Pro Thr Thr Glu Gln Leu
50 55 60Arg Thr Phe Asn Thr Thr Gly Arg Ser Tyr Arg Ser Leu Asp Ala Gly65 70 75 80Leu Ile Ser Leu Ile His Ser Leu Pro Asn Thr Cys His Pro Met Asp
85 90 95Val Leu Arg Thr Ala Val Ser Tyr Met Gly Thr Phe Asp Pro Asp Pro
100 105 110Phe Thr Arg Asp Ala Asp His Ile Arg Ser Ile Gly His Asn Leu Leu
115 120 125Ala Gln Leu Pro Met Val Val Ala Met Asp Ile Arg Arg Arg Ser Gly
130 135 140Glu Glu Ile Ile Ala Pro Asp His Asn Lys Gly Ile Ala Ser Asn Phe145 150 155 160Leu Ser Met Val Phe Gly Asn Asp Asp Gly Ser Val Ala Asn Ser Ala
165 170 175Asp Asp Ile Arg Asp Phe Glu Arg Ser Leu Ile Leu Tyr Ala Glu His
180 185 190Ser Phe Asn Ala Ser Thr Phe Ser Ala Arg Val Ile Ser Ser Thr Arg
195 200 205Ser Asp Thr Tyr Ser Ala Ile Thr Gly Ala Ile Gly Ala Leu Lys Gly
210 215 220Pro Leu His Gly Gly Ala Asn Glu Phe Val Met His Thr Met Leu Asp225 230 235 240Ile Asp Asp Pro Asn Asn Ala Ala Asp Trp Met Gly Lys Ala Leu Asp
245 250 255Arg Lys Glu Arg Ile Met Gly Phe Gly His Arg Val Tyr Lys Asn Gly
260 265 270Asp Ser Arg Val Pro Ser Met Glu Lys Ser Met Arg Ser Leu Ala Ala
275 280 285Arg His Arg Gly Gln Lys Trp Val His Met Tyr Glu Ser Met Gln Glu
290 295 300Val Met Glu Ala Arg Thr Gly Ile Lys Pro Asn Leu Asp Phe Pro Ala305 310 315 320Gly Pro Ala Tyr Tyr Met Leu Gly Phe Pro Val Asp Phe Phe Thr Pro
325 330 335Leu Phe Val Leu Ala Arg Val Ser Gly Trp Thr Ala His Ile Val Glu
340 345 350Gln Phe Glu Asn Asn Ala Leu Ile Arg Pro Leu Ser Ala Tyr Asn Gly
355 360 365Val Glu Glu Arg Glu Val Val Pro Ile Ser Glu Arg Thr
370 375 380<210>621<211>1038<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1015)<223>RXN02333<400>621tcttgcatgc cgtgcaaagc ctgcctgacc tggatgatct tgatcagctc aacatcgaag 60tcgacataag caaccaggcc gcgacgaaag cggggctgtt atg aat ctc ttt tcg 115
Met Asn Leu Phe Ser
1 5aat ggt gtt gat gtg ggg agg cgt cga caa gca ttt aaa gcg gca ctc 163Asn Gly Val Asp Val Gly Arg Arg Arg Gln Ala Phe Lys Ala Ala Leu
10 15 20gcc gca ccc cac atc gcc cgg ctg ccc ggc gca ttc tcc cct ctg att 211Ala Ala Pro His Ile Ala Arg Leu Pro Gly Ala Phe Ser Pro Leu Ile
25 30 35gcg cgc tcc atc gaa gaa gcc ggc ttc gaa ggc gtc tac gtt tcc ggc 259Ala Arg Ser Ile Glu Glu Ala Gly Phe Glu Gly Val Tyr Val Ser Gly
40 45 50gcc gtc ata gct gct gac ctg gca cta ccc gat atc ggc ttg acg acg 307Ala Val Ile Ala Ala Asp Leu Ala Leu Pro Asp Ile Gly Leu Thr Thr
55 60 65ctg acc gaa gtc gcc cac cgc gcg cgg caa att gcg cgc gtc aca gac 355Leu Thr Glu Val Ala His Arg Ala Arg Gln Ile Ala Arg Val Thr Asp70 75 80 85cta gga gtg ctt gtc gac gcc gac acc ggc ttt ggc gaa ccc atg tcg 403Leu Gly Val Leu Val Asp Ala Asp Thr Gly Phe Gly Glu Pro Met Ser
90 95 100gcc gca cgc acc gtc gcc gaa ttg gag gac gcc ggt gtg gcc gga tgc 451Ala Ala Arg Thr Val Ala Glu Leu Glu Asp Ala Gly Val Ala Gly Cys
105 110 115cac ctt gaa gac caa gtc aac ccc aaa cgt tgc ggg cac ttg gac ggc 499His Leu Glu Asp Gln Val Asn Pro Lys Arg Cys Gly His Leu Asp Gly
120 125 130aaa gaa gtc gtg cgc aca gac gtg atg gtt cga cgc atc gca gcc gcc 547Lys Glu Val Val Arg Thr Asp Val Met Val Arg Arg Ile Ala Ala Ala
135 140 145gtc tcg gcc cgg cgc gac ccg aac ttt gtc atc tgc gcc cgc acc gac 595Val Ser Ala Arg Arg Asp Pro Asn Phe Val Ile Cys Ala Arg Thr Asp150 155 160 165gcc gct gga gtg gaa gga atc gac gcc gcc att gag cgc gcg aaa gcc 643Ala Ala Gly Val Glu Gly Ile Asp Ala Ala Ile Glu Arg Ala Lys Ala
170 175 180tac tta gat gcg ggc gcc gac atg att ttc acc gaa gcc ctc cac agc 691Tyr Leu Asp Ala Gly Ala Asp Met Ile Phe Thr Glu Ala Leu His Ser
185 190 195gaa gcc gac ttc cga tac ttc cgg cac gcc atc cct gat gcc ttg ttg 739Glu Ala Asp Phe Arg Tyr Phe Arg His Ala Ile Pro Asp Ala Leu Leu
200 205 210ctg gcg aat atg acc gaa ttt ggc aaa acg acg ctg ctg tca gcc gac 787Leu Ala Asn Met Thr Glu Phe Gly Lys Thr Thr Leu Leu Ser Ala Asp
215 220 225gtg ttg gaa gag att ggc tac aac gcc gtg atc tac ccc gtg acc acg 835Val Leu Glu Glu Ile Gly Tyr Asn Ala Val Ile Tyr Pro Val Thr Thr230 235 240 245ctg cgt att gcc atg gga caa gta gaa caa gca cta gcc gaa atc aaa 883Leu Arg Ile Ala Met Gly Gln Val Glu Gln Ala Leu Ala Glu Ile Lys
250 255 260gaa cac ggt acc caa gaa gga tgg ctg gac cgc atg caa cac cgc agc 931Glu His Gly Thr Gln Glu Gly Trp Leu Asp Arg Met Gln His Arg Ser
265 270 275aga tta tat gag ctc ctg cga tac gaa gac tac aac gtc ttt gac cag 979Arg Leu Tyr Glu Leu Leu Arg Tyr Glu Asp Tyr Asn Val Phe Asp Gln
280 285 290cac att ttc acc tac aga aaa gga gaa aac aat gag tgacagccaa 1025His Ile Phe Thr Tyr Arg Lys Gly Glu Asn Asn Glu
295 300 305gtccgcaaag gac 1038<210>622<211>305<212>PRT<213>谷氨酸棒杆菌<400>622Met Asn Leu Phe Ser Asn Gly Val Asp Val Gly Arg Arg Arg Gln Ala1 5 10 15Phe Lys Ala Ala Leu Ala Ala Pro His Ile Ala Arg Leu Pro Gly Ala
20 25 30Phe Ser Pro Leu Ile Ala Arg Ser Ile Glu Glu Ala Gly Phe Glu Gly
35 40 45Val Tyr Val Ser Gly Ala Val Ile Ala Ala Asp Leu Ala Leu Pro Asp
50 55 60Ile Gly Leu Thr Thr Leu Thr Glu Val Ala His Arg Ala Arg Gln Ile65 70 75 80Ala Arg Val Thr Asp Leu Gly Val Leu Val Asp Ala Asp Thr Gly Phe
85 90 95Gly Glu Pro Met Ser Ala Ala Arg Thr Val Ala Glu Leu Glu Asp Ala
100 105 110Gly Val Ala Gly Cys His Leu Glu Asp Gln Val Asn Pro Lys Arg Cys
115 120 125Gly His Leu Asp Gly Lys Glu Val Val Arg Thr Asp Val Met Val Arg
130 135 140Arg Ile Ala Ala Ala Val Ser Ala Arg Arg Asp Pro Asn Phe Val Ile145 150 155 160Cys Ala Arg Thr Asp Ala Ala Gly Val Glu Gly Ile Asp Ala Ala Ile
165 170 175Glu Arg Ala Lys Ala Tyr Leu Asp Ala Gly Ala Asp Met Ile Phe Thr
180 185 190Glu Ala Leu His Ser Glu Ala Asp Phe Arg Tyr Phe Arg His Ala Ile
195 200 205Pro Asp Ala Leu Leu Leu Ala Asn Met Thr Glu Phe Gly Lys Thr Thr
210 215 220Leu Leu Ser Ala Asp Val Leu Glu Glu Ile Gly Tyr Asn Ala Val Ile225 230 235 240Tyr Pro Val Thr Thr Leu Arg Ile Ala Met Gly Gln Val Glu Gln Ala
245 250 255Leu Ala Glu Ile Lys Glu His Gly Thr Gln Glu Gly Trp Leu Asp Arg
260 265 270Met Gln His Arg Ser Arg Leu Tyr Glu Leu Leu Arg Tyr Glu Asp Tyr
275 280 285Asn Val Phe Asp Gln His Ile Phe Thr Tyr Arg Lys Gly Glu Asn Asn
290 295 300Glu305<210>623<211>242<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(219)<223>FRXA02333<400>623gag att ggc tac aac gcc gtg atc tac ccc gtg acc acg ctg cgt att 48Glu Ile Gly Tyr Asn Ala Val Ile Tyr Pro Val Thr Thr Leu Arg Ile1 5 10 15gcc atg gga caa gta gaa caa gca cta gcc gaa atc aaa gaa cac ggt 96Ala Met Gly Gln Val Glu Gln Ala Leu Ala Glu Ile Lys Glu His Gly
20 25 30acc caa gaa gga tgg ctg gac cgc atg caa cac cgc agc aga tta tat 144Thr Gln Glu Gly Trp Leu Asp Arg Met Gln His Arg Ser Arg Leu Tyr
35 40 45gag ctc ctg cga tac gaa gac tac aac gtc ttt gac cag cac att ttc 192Glu Leu Leu Arg Tyr Glu Asp Tyr Asn Val Phe Asp Gln His Ile Phe
50 55 60acc tac aga aaa gga gaa aac aat gag tgacagccaa gtccgcaaag 239Thr Tyr Arg Lys Gly Glu Asn Asn Glu65 70gac 242<210>624<211>73<212>PRT<213>谷氨酸棒杆菌<400>624Glu Ile Gly Tyr Asn Ala Val Ile Tyr Pro Val Thr Thr Leu Arg Ile1 5 10 15Ala Met Gly Gln Val Glu Gln Ala Leu Ala Glu Ile Lys Glu His Gly
20 25 30Thr Gln Glu Gly Trp Leu Asp Arg Met Gln His Arg Ser Arg Leu Tyr
35 40 45Glu Leu Leu Arg Tyr Glu Asp Tyr Asn Val Phe Asp Gln His Ile Phe
50 55 60Thr Tyr Arg Lys Gly Glu Asn Asn Glu65 70<210>625<211>513<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(490)<223>RXA00030<400>625taaaaacgcc actttggtgt cgataacacc gaacttccga acataaaaga tgcgttggtg 60ctctctgcac aatgtttctt tcggaactat tctggcgacc atg cga att gaa atc 115
Met Arg Ile Glu Ile
1 5aca agc gta ttt gtt gat gac cag gcc aaa gca ctc gat ttc tac acc 163Thr Ser Val Phe Val Asp Asp Gln Ala Lys Ala Leu Asp Phe Tyr Thr
10 15 20acg aag ctc gga ttt gag ctc aaa cac gat gta act gct ggt gac tac 211Thr Lys Leu Gly Phe Glu Leu Lys His Asp Val Thr Ala Gly Asp Tyr
25 30 35cgc tgg ttg act gtt gtt gat cca gaa aac cca gat ggt gtg cag ctt 259Arg Trp Leu Thr Val Val Asp Pro Glu Asn Pro Asp Gly Val Gln Leu
40 45 50ttg ttg gaa cca aac cag cac cca gat gca gcg act tac caa gct gga 307Leu Leu Glu Pro Asn Gln His Pro Asp Ala Ala Thr Tyr Gln Ala Gly
55 60 65att aaa cga gac ggt att ccc gct aca cag ttt tat gtt gat gat gtg 355Ile Lys Arg Asp Gly Ile Pro Ala Thr Gln Phe Tyr Val Asp Asp Val70 75 80 85cag gaa gaa tat gac agc ctc aag gat aaa ggc gtg gat ttc atc atg 403Gln Glu Glu Tyr Asp Ser Leu Lys Asp Lys Gly Val Asp Phe Ile Met
90 95 100gaa cca acc gat gtg ggc cct tca gtg att gcc att ctc gat gac acc 451Glu Pro Thr Asp Val Gly Pro Ser Val Ile Ala Ile Leu Asp Asp Thr
105 110 115gta gga aac cta att cag att gtt caa ttg aag cag aac taaccccgtg 500Val Gly Asn Leu Ile Gln Ile Val Gln Leu Lys Gln Asn
120 125 130gaatgacaaa atc 513<210>626<211>130<212>PRT<213>谷氨酸棒杆菌<400>626Met Arg Ile Glu Ile Thr Ser Val Phe Val Asp Asp Gln Ala Lys Ala1 5 10 15Leu Asp Phe Tyr Thr Thr Lys Leu Gly Phe Glu Leu Lys His Asp Val
20 25 30Thr Ala Gly Asp Tyr Arg Trp Leu Thr Val Val Asp Pro Glu Asn Pro
35 40 45Asp Gly Val Gln Leu Leu Leu Glu Pro Asn Gln His Pro Asp Ala Ala
50 55 60Thr Tyr Gln Ala Gly Ile Lys Arg Asp Gly Ile Pro Ala Thr Gln Phe65 70 75 80Tyr Val Asp Asp Val Gln Glu Glu Tyr Asp Ser Leu Lys Asp Lys Gly
85 90 95Val Asp Phe Ile Met Glu Pro Thr Asp Val Gly Pro Sar Val Ila Ala
100 105 110Ile Leu Asp Asp Thr Val Gly Asn Leu Ile Gln Ile Val Gln Leu Lys
115 120 125Gln Asn
130<210>627<211>2334<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2311)<223>RXN00148<400>627ctttgagggc agcgcgcatg cgcccgatgg ttatttgaac atgacaattg atgccgcggc 60gacgctggct gacctgetag atgctttggg agcttaaatc atg acg tcg atc cct 115
Met Thr Ser Ile Pro
1 5aat ttt tca gac atc cca ttg act gct gag aca cgt gca tcg gag tca 163Asn Phe Ser Asp Ile Pro Leu Thr Ala Glu Thr Arg Ala Ser Glu Ser
10 15 20cac aac gtt gac gcc ggc aag gtg tgg aac act ccc gaa ggc att gat 211His Asn Val Asp Ala Gly Lys Val Trp Asn Thr Pro Glu Gly Ile Asp
25 30 35gtc aag cgc gta ttc acg cag gct gac cgc gac gag gcg caa gcg gcg 259Val Lys Arg Val Phe Thr Gln Ala Asp Arg Asp Glu Ala Gln Ala Ala
40 45 50gga cat ccg gtg gat tct ttg cca ggt caa aag cca ttt atg cgc ggg 307Gly His Pro Val Asp Ser Leu Pro Gly Gln Lys Pro Phe Met Arg Gly
55 60 65ccg tac cca act atg tac acc aat cag ccg tgg acg att cgc cag tac 355Pro Tyr Pro Thr Met Tyr Thr Asn Gln Pro Trp Thr Ile Arg Gln Tyr70 75 80 85gca ggc ttt tca acc gcc gcg gaa tcc aat gcg ttt tat cgg agg aac 403Ala Gly Phe Ser Thr Ala Ala Glu Ser Asn Ala Phe Tyr Arg Arg Asn
90 95 100ctt gct gcg ggt caa aaa ggt ttg tcg gtt gcg ttc gat cta gcg acc 451Leu Ala Ala Gly Gln Lys Gly Leu Ser Val Ala Phe Asp Leu Ala Thr
105 110 115cac cgc ggt tat gac tcg gat aat gag cgc gtg gtc ggc gat gtg ggt 499His Arg Gly Tyr Asp Ser Asp Asn Glu Arg Val Val Gly Asp Val Gly
120 125 130atg gcc ggc gtg gcg att gat tcg att ttg gat atg cgt cag ctg ttt 547Met Ala Gly Val Ala Ile Asp Ser Ile Leu Asp Met Arg Gln Leu Phe
135 140 145gat ggc att gat ttg tcc agc gtg tcg gtg tcg atg acc atg aat ggc 595Asp Gly Ile Asp Leu Ser Ser Val Ser Val Ser Met Thr Met Asn Gly150 155 160 165gct gtg ctg ccg att ctt gcg ttc tat atc gtg gcg gct gag gaa caa 643Ala Val Leu Pro Ile Leu Ala Phe Tyr Ile Val Ala Ala Glu Glu Gln
170 175 180ggt gtg ggt ccg gag cag ctt gcg ggc acg atc cag aat gac atc ttg 691Gly Val Gly Pro Glu Gln Leu Ala Gly Thr Ile Gln Asn Asp Ile Leu
185 190 195aaa gaa ttt atg gtg cgc aac acc tat att tat ccg ccg aag ccg tcg 739Lys Glu Phe Met Val Arg Asn Thr Tyr Ile Tyr Pro Pro Lys Pro Ser
200 205 210atg cgc atc att tcc aac atc ttt gag tac acc tcc ttg aag atg cca 787Met Arg Ile Ile Ser Asn Ile Phe Glu Tyr Thr Ser Leu Lys Met Pro
215 220 225cgt ttt aac tcc att tcg att tct ggc tat cac atc cag gaa gcg gga 835Arg Phe Asn Ser Ile Ser Ile Ser Gly Tyr His Ile Gln Glu Ala Gly230 235 240 245gcg act gcc gat ttg gag ctg gcc tac act ctg gcg gat ggt att gaa 883Ala Thr Ala Asp Leu Glu Leu Ala Tyr Thr Leu Ala Asp Gly Ile Glu
250 255 260tac atc cgt gca ggt aaa gag gta ggc ctt gac gtg gat aag ttc gcg 931Tyr Ile Arg Ala Gly Lys Glu Val Gly Leu Asp Val Asp Lys Phe Ala
265 270 275cct cgt ctg tcc ttc ttc tgg ggt att tct atg tac acc ttc atg gag 979Pro Arg Leu Ser Phe Phe Trp Gly Ile Ser Met Tyr Thr Phe Met Glu
280 285 290atc gca aag ctg cgt gcg gga cga ctg ctg tgg agc gag ttg gtg gca 1027Ile Ala Lys Leu Arg Ala Gly Arg Leu Leu Trp Ser Glu Leu Val Ala
295 300 305aaa ttc gat ccg aaa aac gcc aag tcc cag tcg ctg cgc acg cac tcg 1075Lys Phe Asp Pro Lys Asn Ala Lys Ser Gln Ser Leu Arg Thr His Ser310 315 320 325cag acc tct ggt tgg tcg ttg acc gcg cag gat gtg tac aac aac gtc 1123Gln Thr Ser Gly Trp Ser Leu Thr Ala Gln Asp Val Tyr Asn Asn Val
330 335 340gcc cgc acc gcg att gag gcg atg gct gca acc cag ggc cac acc cag 1171Ala Arg Thr Ala Ile Glu Ala Met Ala Ala Thr Gln Gly His Thr Gln
345 350 355tcg ctg cac acc aat gca ctt gat gag gcg ttg gcg ctg ccc acc gat 1219Ser Leu His Thr Asn Ala Leu Asp Glu Ala Leu Ala Leu Pro Thr Asp
360 365 370ttc tct gct cgt atc gcc cga aac acc cag ctg ttg ctg cag cag gaa 1267Phe Ser Ala Arg Ile Ala Arg Asn Thr Gln Leu Leu Leu Gln Gln Glu
375 380 385tct ggc acg gtg cgt cca gtt gat cca ggg gcg ggc tcc tat tac gtg 1315Ser Gly Thr Val Arg Pro Val Asp Pro Trp Ala Gly Ser Tyr Tyr Val390 395 400 405gag tgg ttg acc aat gag ctg gct aac cgc gcg cgc aag cac atc gat 1363Glu Trp Leu Thr Asn Glu Leu Ala Asn Arg Ala Arg Lys His Ile Asp
410 415 420gag gtg gag gaa gcc ggc gga atg gcg cag gcc acc gcg cag gga att 1411Glu Val Glu Glu Ala Gly Gly Met Ala Gln Ala Thr Ala Gln Gly Ile
425 430 435cct aag ctg cgc att gag gaa tca gcg gca cgc acc cag gct cgc att 1459Pro Lys Leu Arg Ile Glu Glu Ser Ala Ala Arg Thr Gln Ala Arg Ile
440 445 450gat tcc ggc cgc cag gcg ctg atc ggc gtg aat cgc tac gtg gcg gaa 1507Asp Ser Gly Arg Gln Ala Leu Ile Gly Val Asn Arg Tyr Val Ala Glu
455 460 465gaa gat gag gaa att gaa gtc ctc aag gtt gac aac acc aag gtt cgc 1555Glu Asp Glu Glu Ile Glu Val Leu Lys Val Asp Asn Thr Lys Val Arg470 475 480 485gca gaa cag ttg gct aaa ctc gcg caa ctg aaa gca gag cgc aac gat 1603Ala Glu Gln Leu Ala Lys Leu Ala Gln Leu Lys Ala Glu Arg Asn Asp
490 495 500gcg gaa gtc aag gct gcg ctg gat gcg ttg aca gct gct gcc cgc aac 1651Ala Glu Val Lys Ala Ala Leu Asp Ala Leu Thr Ala Ala Ala Arg Asn
505 510 515gag cat aaa gag cca ggg gat ttg gat cag aac ctg ctc aaa ctt gcc 1699Glu His Lys Glu Pro Gly Asp Leu Asp Gln Asn Leu Leu Lys Leu Ala
520 525 530gtc gat gct gcg cgc gca aaa gct acc att gga gag atc tcc gat gct 1747Val Asp Ala Ala Arg Ala Lys Ala Thr Ile Gly Glu Ile Ser Asp Ala
535 540 545ttg gaa gtt gtc ttt ggc cgc cac gaa gca gaa atc agg acg ctg tct 1795Leu Glu Val Val Phe Gly Arg His Glu Ala Glu Ile Arg Thr Leu Ser550 555 560 565ggc gtg tac aag gat gag gtt gga aag gaa ggc aca gtg agc aac gtc 1843Gly Val Tyr Lys Asp Glu Val Gly Lys Glu Gly Thr Val Ser Asn Val
570 575 580gaa cgc gcg atc gcc ctg gct gac gcc ttt gag gct gag gaa ggc cgc 1891Glu Arg Ala Ile Ala Leu Ala Asp Ala Phe Glu Ala Glu Glu Gly Arg
585 590 595cgc cca cgt atc ttt att gcc aag atg ggc cag gat gga cat gac cgt 1939Arg Pro Arg Ile Phe Ile Ala Lys Met Gly Gln Asp Gly His Asp Arg
600 605 610gga cag aag gtt gtc gcg tct gcc tat gct gac ctg ggc atg gac gtg 1987Gly Gln Lys Val Val Ala Ser Ala Tyr Ala Asp Leu Gly Met Asp Val
615 620 625gat gtt gga ccg ctg ttt caa act cca gcc gaa gct gcc cgc gcc gcc 2035Asp Val Gly Pro Leu Phe Gln Thr Pro Ala Glu Ala Ala Arg Ala Ala630 635 640 645gtg gac gcc gat gtt cac gtg gtg ggt atg tct tcg ctg gca gca ggc 2083Val Asp Ala Asp Val His Val Val Gly Met Ser Ser Leu Ala Ala Gly
650 655 660cac ctc acc ttg ctg ccc gag ctg aag aaa gaa ctt gca gct ctt ggc 2131His Leu Thr Leu Leu Pro Glu Leu Lys Lys Glu Leu Ala Ala Leu Gly
665 670 675cgc gat gac att ctg gtc acc gtg ggc ggc gtc att ccg ccg ggc gat 2179Arg Asp Asp Ile Leu Val Thr Val Gly Gly Val Ile Pro Pro Gly Asp
680 685 690ttc cag gat ctc tac gat atg ggt gcc gcc gcg att tac cct tca gga 2227Phe Gln Asp Leu Tyr Asp Met Gly Ala Ala Ala Ile Tyr Pro Ser Gly
695 700 705acc gtc atc gcg gag tcg gcg atc gat ctg atc acc cga ctc gcc gca 2275Thr Val Ile Ala Glu Ser Ala Ile Asp Leu Ile Thr Arg Leu Ala Ala710 715 720 725cac ctg ggc ttt gac ctg gat gtg gat gtg aat gaa tgatcacggt 2321His Leu Gly Phe Asp Leu Asp Val Asp Val Asn Glu
730 735ttcctagaag aca 2334<210>628<211>737<212>PRT<213>谷氨酸棒杆菌<400>628Met Thr Ser Ile Pro Asn Phe Ser Asp Ile Pro Leu Thr Ala Glu Thr1 5 10 15Arg Ala Ser Glu Ser His Asn Val Asp Ala Gly Lys Val Trp Asn Thr
20 25 30Pro Glu Gly Ile Asp Val Lys Arg Val Phe Thr Gln Ala Asp Arg Asp
35 40 45Glu Ala Gln Ala Ala Gly His Pro Val Asp Ser Leu Pro Gly Gln Lys
50 55 60Pro Phe Met Arg Gly Pro Tyr Pro Thr Met Tyr Thr Asn Gln Pro Trp65 70 75 80Thr Ile Arg Gln Tyr Ala Gly Phe Ser Thr Ala Ala Glu Ser Asn Ala
85 90 95Phe Tyr Arg Arg Asn Leu Ala Ala Gly Gln Lys Gly Leu Ser Val Ala
100 105 110Phe Asp Leu Ala Thr His Arg Gly Tyr Asp Ser Asp Asn Glu Arg Val
115 120 125Val Gly Asp Val Gly Met Ala Gly Val Ala Ile Asp Ser Ile Leu Asp
130 135 140Met Arg Gln Leu Phe Asp Gly Ile Asp Leu Ser Ser Val Ser Val Ser145 150 155 160Met Thr Met Asn Gly Ala Val Leu Pro Ile Leu Ala Phe Tyr Ile Val
165 170 175Ala Ala Glu Glu Gln Gly Val Gly Pro Glu Gln Leu Ala Gly Thr Ile
180 185 190Gln Asn Asp Ile Leu Lys Glu Phe Met Val Arg Asn Thr Tyr Ile Tyr
190 200 205Pro Pro Lys Pro Ser Met Arg Ile Ile Ser Asn Ile Phe Glu Tyr Thr
210 215 220Ser Leu Lys Met Pro Arg Phe Asn Ser Ile Ser Ile Ser Gly Tyr His225 230 235 240Ile Gln Glu Ala Gly Ala Thr Ala Asp Leu Glu Leu Ala Tyr Thr Leu
245 250 255Ala Asp Gly Ile Glu Tyr Ile Arg Ala Gly Lys Glu Val Gly Leu Asp
260 265 270Val Asp Lys Phe Ala Pro Arg Leu Ser Phe Phe Trp Gly Ile Ser Met
275 280 285Tyr Thr Phe Met Glu Ile Ala Lys Leu Arg Ala Gly Arg Leu Leu Trp
290 295 300Ser Glu Leu Val Ala Lys Phe Asp Pro Lys Asn Ala Lys Ser Gln Ser305 310 315 320Leu Arg Thr His Ser Gln Thr Ser Gly Trp Ser Leu Thr Ala Gln Asp
325 330 335Val Tyr Asn Asn Val Ala Arg Thr Ala Ile Glu Ala Met Ala Ala Thr
340 345 350Gln Gly His Thr Gln Ser Leu His Thr Asn Ala Leu Asp Glu Ala Leu
355 360 365Ala Leu Pro Thr Asp Phe Ser Ala Arg Ile Ala Arg Asn Thr Gln Leu
370 375 380Leu Leu Gln Gln Glu Ser Gly Thr Val Arg Pro Val Asp Pro Trp Ala385 390 395 400Gly Ser Tyr Tyr Val Glu Trp Leu Thr Asn Glu Leu Ala Asn Arg Ala
405 410 415Arg Lys His Ile Asp Glu Val Glu Glu Ala Gly Gly Met Ala Gln Ala
420 425 430Thr Ala Gln Gly Ile Pro Lys Leu Arg Ile Glu Glu Ser Ala Ala Arg
435 440 445Thr Gln Ala Arg Ile Asp Ser Gly Arg Gln Ala Leu Ile Gly Val Asn
450 455 460Arg Tyr Val Ala Glu Glu Asp Glu Glu Ile Glu Val Leu Lys Val Asp465 470 475 480Asn Thr Lys Val Arg Ala Glu Gln Leu Ala Lys Leu Ala Gln Leu Lys
485 490 495Ala Glu Arg Asn Asp Ala Glu Val Lys Ala Ala Leu Asp Ala Leu Thr
500 505 510Ala Ala Ala Arg Asn Glu His Lys Glu Pro Gly Asp Leu Asp Gln Asn
515 520 525Leu Leu Lys Leu Ala Val Asp Ala Ala Arg Ala Lys Ala Thr Ile Gly
530 535 540Glu Ile Ser Asp Ala Leu Glu Val Val Phe Gly Arg His Glu Ala Glu545 550 555 560Ile Arg Thr Leu Ser Gly Val Tyr Lys Asp Glu Val Gly Lys Glu Gly
565 570 575Thr Val Ser Asn Val Glu Arg Ala Ile Ala Leu Ala Asp Ala Phe Glu
580 585 590Ala Glu Glu Gly Arg Arg Pro Arg Ile Phe Ile Ala Lys Met Gly Gln
595 600 605Asp Gly His Asp Arg Gly Gln Lys Val Val Ala Ser Ala Tyr Ala Asp
610 615 620Leu Gly Met Asp Val Asp Val Gly Pro Leu Phe Gln Thr Pro Ala Glu625 630 635 640Ala Ala Arg Ala Ala Val Asp Ala Asp Val His Val Val Gly Met Ser
645 650 655Ser Leu Ala Ala Gly His Leu Thr Leu Leu Pro Glu Leu Lys Lys Glu
660 665 670Leu Ala Ala Leu Gly Arg Asp Asp Ile Leu Val Thr Val Gly Gly Val
675 680 685Ile Pro Pro Gly Asp Phe Gln Asp Leu Tyr Asp Met Gly Ala Ala Ala
690 695 700Ile Tyr Pro Ser Gly Thr Val Ile Ala Glu Ser Ala Ile Asp Leu Ile705 710 715 720Thr Arg Leu Ala Ala His Leu Gly Phe Asp Leu Asp Val Asp Val Asn
725 730 735Glu<210>629<211>2098<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2098)<223>FRXA00148<400>629ctttgagggc agcgcgcatg cgcccgatgg ttatttgaac atgacaattg atgccgcggc 60gacgctggct gacctgctag atgctttggg agcttaaatc atg acg tcg atc cct 115
Met Thr Ser Ile Pro
1 5aat ttt tca gac atc cca ttg act gct gag aca cgt gca tcg gag tca 163Asn Phe Ser Asp Ile Pro Leu Thr Ala Glu Thr Arg Ala Ser Glu Ser
10 15 20cac aac gtt gac gcc ggc aag gtg tgg aac act ccc gaa ggc att gat 211His Asn Val Asp Ala Gly Lys Val Trp Asn Thr Pro Glu Gly Ile Asp
25 30 35gtc aag cgc gta ttc acg cag gct gac cgc gac gag gcg caa gcg gcg 259Val Lys Arg Val Phe Thr Gln Ala Asp Arg Asp Glu Ala Gln Ala Ala
40 45 50gga cat ccg gtg gat tct ttg cca ggt caa aag cca ttt atg cgc ggg 307Gly His Pro Val Asp Ser Leu Pro Gly Gln Lys Pro Phe Met Arg Gly
55 60 65ccg tac cca act atg tac acc aat cag ccg tgg acg att cgc cag tac 355Pro Tyr Pro Thr Met Tyr Thr Asn Gln Pro Trp Thr Ile Arg Gln Tyr70 75 80 85gca ggc ttt tca acc gcc gcg gaa tcc aat gcg ttt tat cgg agg aac 403Ala Gly Phe Ser Thr Ala Ala Glu Ser Asn Ala Phe Tyr Arg Arg Asn
90 95 100ctt gct gcg ggt caa aaa ggt ttg tcg gtt gcg ttc gat cta gcg acc 451Leu Ala Ala Gly Gln Lys Gly Leu Ser Val Ala Phe Asp Leu Ala Thr
105 110 115cac cgc ggt tat gac tcg gat aat gag cgc gtg gtc ggc gat gtg ggt 499His Arg Gly Tyr Asp Ser Asp Asn Glu Arg Val Val Gly Asp Val Gly
120 125 130atg gcc ggc gtg gcg att gat tcg att ttg gat atg cgt cag ctg ttt 547Met Ala Gly Val Ala Ile Asp Ser Ile Leu Asp Met Arg Gln Leu Phe
135 140 145gat ggc att gat ttg tcc agc gtg tcg gtg tcg atg acc atg aat ggc 595Asp Gly Ile Asp Leu Ser Ser Val Ser Val Ser Met Thr Met Asn Gly150 155 160 165gct gtg ctg ccg att ctt gcg ttc tat atc gtg gcg gct gag gaa caa 643Ala Val Leu Pro Ile Leu Ala Phe Tyr Ile Val Ala Ala Glu Glu Gln
170 175 180ggt gtg ggt ccg gag cag ctt gcg ggc acg atc cag aat gac atc ttg 691Gly Val Gly Pro Glu Gln Leu Ala Gly Thr Ile Gln Asn Asp Ile Leu
185 190 195aaa gaa ttt atg gtg cgc aac acc tat att tat ccg ccg aag ccg tcg 739Lys Glu Phe Met Val Arg Asn Thr Tyr Ile Tyr Pro Pro Lys Pro Ser
200 205 210atg cgc atc att tcc aac atc ttt gag tac acc tcc ttg aag atg cca 787Met Arg Ile Ile Ser Asn Ile Phe Glu Tyr Thr Ser Leu Lys Met Pro
215 220 225cgt ttt aac tcc att tcg att tct ggc tat cac atc cag gaa gcg gga 835Arg Phe Asn Ser Ile Ser Ile Ser Gly Tyr His Ile Gln Glu Ala Gly230 235 240 245gcg act gcc gat ttg gag ctg gcc tac act ctg gcg gat ggt att gaa 883Ala Thr Ala Asp Leu Glu Leu Ala Tyr Thr Leu Ala Asp Gly Ile Glu
250 255 260tac atc cgt gca ggt aaa gag gta ggc ctt gac gtg gat aag ttc gcg 931Tyr Ile Arg Ala Gly Lys Glu Val Gly Leu Asp Val Asp Lys Phe Ala
265 270 275cct cgt ctg tcc ttc ttc tgg ggt att tct atg tac acc ttc atg gag 979Pro Arg Leu Ser Phe Phe Trp Gly Ile Ser Met Tyr Thr Phe Met Glu
280 285 290atc gca aag ctg cgt gcg gga cga ctg ctg tgg agc gag ttg gtg gca 1027Ile Ala Lys Leu Arg Ala Gly Arg Leu Leu Trp Ser Glu Leu Val Ala
295 300 305aaa ttc gat ccg aaa aac gcc aag tcc cag tcg ctg cgc acg cac tcg 1075Lys Phe Asp Pro Lys Asn Ala Lys Ser Gln Ser Leu Arg Thr His Ser310 315 320 325cag acc tct ggt tgg tcg ttg acc gcg cag gat gtg tac aac aac gtc 1123Gln Thr Ser Gly Trp Ser Leu Thr Ala Gln Asp Val Tyr Asn Asn Val
330 335 340gcc cgc acc gcg att gag gcg atg gct gca acc cag ggc cac acc cag 1171Ala Arg Thr Ala Ile Glu Ala Met Ala Ala Thr Gln Gly His Thr Gln
345 350 355tcg ctg cac acc aat gca ctt gat gag gcg ttg gcg ctg ccc acc gat 1219Ser Leu His Thr Asn Ala Leu Asp Glu Ala Leu Ala Leu Pro Thr Asp
360 365 370ttc tct gct cgt atc gcc cga aac acc cag ctg ttg ctg cag cag gaa 1267Phe Ser Ala Arg Ile Ala Arg Asn Thr Gln Leu Leu Leu Gln Gln Glu
375 380 385tct ggc acg gtg cgt cca gtt gat cca tgg gcg ggc tcc tat tac gtg 1315Ser Gly Thr Val Arg Pro Val Asp Pro Trp Ala Gly Ser Tyr Tyr Val390 395 400 405gag tgg ttg acc aat gag ctg gct aac cgc gcg cgc aag cac atc gat 1363Glu Trp Leu Thr Asn Glu Leu Ala Asn Arg Ala Arg Lys His Ile Asp
410 415 420gag gtg gag gaa gcc ggc gga atg gcg cag gcc acc gcg cag gga att 1411Glu Val Glu Glu Ala Gly Gly Met Ala Gln Ala Thr Ala Gln Gly Ile
425 430 435cct aag ctg cgc att gag gaa tca gcg gca cgc acc cag gct cgc att 1459Pro Lys Leu Arg Ile Glu Glu Ser Ala Ala Arg Thr Gln Ala Arg Ile
440 445 450gat tcc ggc cgc cag gcg ctg atc ggc gtg aat cgc tac gtg gcg gaa 1507Asp Ser Gly Arg Gln Ala Leu Ile Gly Val Asn Arg Tyr Val Ala Glu
455 460 465gaa gat gag gaa att gaa gtc ctc aag gtt gac aac acc aag gtt cgc 1555Glu Asp Glu Glu Ile Glu Val Leu Lys Val Asp Asn Thr Lys Val Arg470 475 480 485gca gaa cag ttg gct aaa ctc gcg caa ctg aaa gca gag cgc aac gat 1603Ala Glu Gln Leu Ala Lys Leu Ala Gln Leu Lys Ala Glu Arg Asn Asp
490 495 500gcg gaa gtc aag gct gcg ctg gat gcg ttg aca gct gct gcc cgc aac 1651Ala Glu Val Lys Ala Ala Leu Asp Ala Leu Thr Ala Ala Ala Arg Asn
505 510 515gag cat aaa gag cca ggg gat ttg gat cag aac ctg ctc aaa ctt gcc 1699Glu His Lys Glu Pro Gly Asp Leu Asp Gln Asn Leu Leu Lys Leu Ala
520 525 530gtc gat gct gcg cgc gca aaa gct acc att gga gag atc tcc gat gct 1747Val Asp Ala Ala Arg Ala Lys Ala Thr Ile Gly Glu Ile Ser Asp Ala
535 540 545ttg gaa gtt gtc ttt ggc cgc cac gaa gca gaa atc agg acg ctg tct 1795Leu Glu Val Val Phe Gly Arg His Glu Ala Glu Ile Arg Thr Leu Ser550 555 560 565ggc gtg tac aag gat gag gtt gga aag gaa ggc aca gtg agc aac gtc 1843Gly Val Tyr Lys Asp Glu Val Gly Lys Glu Gly Thr Val Ser Asn Val
570 575 580gaa cgc gcg atc gcc ctg gct gac gcc ttt gag gct gag gaa ggc cgc 1891Glu Arg Ala Ile Ala Leu Ala Asp Ala Phe Glu Ala Glu Glu Gly Arg
585 590 595cgc cca cgt atc ttt att gcc aag atg ggc cag gat gga cat gac cgt 1939Arg Pro Arg Ile Phe Ile Ala Lys Met Gly Gln Asp Gly His Asp Arg
600 605 610gga cag aag gtt gtc gcg tct gcc tat gct gac ctg ggc atg gac gtg 1987Gly Gln Lys Val Val Ala Ser Ala Tyr Ala Asp Leu Gly Met Asp Val
615 620 625gat gtt gga ccg ctg ttt caa act cca gcc gaa gct gcc cgc gcc gcc 2035Asp Val Gly Pro Leu Phe Gln Thr Pro Ala Glu Ala Ala Arg Ala Ala630 635 640 645gtg gac gcc gat gtt cac gtg gtg ggt atg tct tcg ctg gca gca ggc 2083Val Asp Ala Asp Val His Val Val Gly Met Ser Ser Leu Ala Ala Gly
650 655 660cac ctc acc ttg ctg 2098His Leu Thr Leu Leu
665<210>630<211>666<212>PRT<213>谷氨酸棒杆菌<400>630Met Thr Ser Ile Pro Asn Phe Ser Asp Ile Pro Leu Thr Ala Glu Thr1 5 10 15Arg Ala Ser Glu Ser His Asn Val Asp Ala Gly Lys Val Trp Asn Thr
20 25 30Pro Glu Gly Ile Asp Val Lys Arg Val Phe Thr Gln Ala Asp Arg Asp
35 40 45Glu Ala Gln Ala Ala Gly His Pro Val Asp Ser Leu Pro Gly Gln Lys
50 55 60Pro Phe Met Arg Gly Pro Tyr Pro Thr Met Tyr Thr Asn Gln Pro Trp65 70 75 80Thr Ile Arg Gln Tyr Ala Gly Phe Ser Thr Ala Ala Glu Ser Asn Ala
85 90 95Phe Tyr Arg Arg Asn Leu Ala Ala Gly Gln Lys Gly Leu Ser Val Ala
100 105 110Phe Asp Leu Ala Thr His Arg Gly Tyr Asp Ser Asp Asn Glu Arg Val
115 120 125Val Gly Asp Val Gly Met Ala Gly Val Ala Ile Asp Ser Ile Leu Asp
130 135 140Met Arg Gln Leu Phe Asp Gly Ile Asp Leu Ser Ser Val Ser Val Ser145 150 155 160Met Thr Met Asn Gly Ala Val Leu Pro Ile Leu Ala Phe Tyr Ile Val
165 170 175Ala Ala Glu Glu Gln Gly Val Gly Pro Glu Gln Leu Ala Gly Thr Ile
180 185 190Gln Asn Asp Ile Leu Lys Glu Phe Met Val Arg Asn Thr Tyr Ile Tyr
195 200 205Pro Pro Lys Pro Ser Met Arg Ile Ile Ser Asn Ile Phe Glu Tyr Thr
210 215 220Ser Leu Lys Met Pro Arg Phe Asn Ser Ile Ser Ile Ser Gly Tyr His225 230 235 240Ile Gln Glu Ala Gly Ala Thr Ala Asp Leu Glu Leu Ala Tyr Thr Leu
245 250 255Ala Asp Gly Ile Glu Tyr Ile Arg Ala Gly Lys Glu Val Gly Leu Asp
260 265 270Val Asp Lys Phe Ala Pro Arg Leu Ser Phe Phe Trp Gly Ile Ser Met
275 280 285Tyr Thr Phe Met Glu Ile Ala Lys Leu Arg Ala Gly Arg Leu Leu Trp
290 295 300Ser Glu Leu Val Ala Lys Phe Asp Pro Lys Asn Ala Lys Ser Gln Ser305 310 315 320Leu Arg Thr His Ser Gln Thr Ser Gly Trp Ser Leu Thr Ala Gln Asp
325 330 335Val Tyr Asn Asn Val Ala Arg Thr Ala Ile Glu Ala Met Ala Ala Thr
340 345 350Gln Gly His Thr Gln Ser Leu His Thr Asn Ala Leu Asp Glu Ala Leu
355 360 365Ala Leu Pro Thr Asp Phe Ser Ala Arg Ile Ala Arg Asn Thr Gln Leu
370 375 380Leu Leu Gln Gln Glu Ser Gly Thr Val Arg Pro Val Asp Pro Trp Ala385 390 395 400Gly Ser Tyr Tyr Val Glu Trp Leu Thr Asn Glu Leu Ala Asn Arg Ala
405 410 415Arg Lys His Ile Asp Glu Val Glu Glu Ala Gly Gly Met Ala Gln Ala
420 425 430Thr Ala Gln Gly Ile Pro Lys Leu Arg Ile Glu Glu Ser Ala Ala Arg
435 440 445Thr Gln Ala Arg Ile Asp Ser Gly Arg Gln Ala Leu Ile Gly Val Asn
450 455 460Arg Tyr Val Ala Glu Glu Asp Glu Glu Ile Glu Val Leu Lys Val Asp465 470 475 480Asn Thr Lys Val Arg Ala Glu Gln Leu Ala Lys Leu Ala Gln Leu Lys
485 490 495Ala Glu Arg Asn Asp Ala Glu Val Lys Ala Ala Leu Asp Ala Leu Thr
500 505 510Ala Ala Ala Arg Asn Glu His Lys Glu Pro Gly Asp Leu Asp Gln Asn
515 520 525Leu Leu Lys Leu Ala Val Asp Ala Ala Arg Ala Lys Ala Thr Ile Gly
530 535 540Glu Ile Ser Asp Ala Leu Glu Val Val Phe Gly Arg His Glu Ala Glu545 550 555 560Ile Arg Thr Leu Ser Gly Val Tyr Lys Asp Glu Val Gly Lys Glu Gly
565 570 575Thr Val Ser Asn Val Glu Arg Ala Ile Ala Leu Ala Asp Ala Phe Glu
580 585 590Ala Glu Glu Gly Arg Arg Pro Arg Ile Phe Ile Ala Lys Met Gly Gln
595 600 605Asp Gly His Asp Arg Gly Gln Lys Val Val Ala Ser Ala Tyr Ala Asp
610 615 620Leu Gly Met Asp Val Asp Val Gly Pro Leu Phe Gln Thr Pro Ala Glu625 630 635 640Ala Ala Arg Ala Ala Val Asp Ala Asp Val His Val Val Gly Met Ser
645 650 655Ser Leu Ala Ala Gly His Leu Thr Leu Leu
660 665<210>631<211>1971<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1948)<223>RXA00149<400>631ttttcgtagg taaacacagg tgaaggcttt acaagcttgt gaactcccta cacaaaagca 60atccaatagc tatccataag caagagaaag taagtctacg ttg act gat ctc aca 115
Leu Thr Asp Leu Thr
1 5aag act gcg gtg ccc gag gaa ctt tca gag aac ctc gaa act tgg tac 163Lys Thr Ala Val Pro Glu Glu Leu Ser Glu Asn Leu Glu Thr Trp Tyr
10 15 20aag gct gtg gcc ggt gtt ttc gcg cgc aca cag aaa aaa gac atc ggc 211Lys Ala Val Ala Gly Val Phe Ala Arg Thr Gln Lys Lys Asp Ile Gly
25 30 35gac att gcc gta gat gtg tgg aag aaa ctc atc gtc act aca ccg gat 259Asp Ile Ala Val Asp Val Trp Lys Lys Leu Ile Val Thr Thr Pro Asp
40 45 50ggt gtt gat atc aat ccg ctg tac acc aga gca gat gag tcc cag agg 307Gly Val Asp Ile Asn Pro Leu Tyr Thr Arg Ala Asp Glu Ser Gln Arg
55 60 65aaa ttc act gag gtt cct ggt gag ttt ccc ttc act agg gga acc act 355Lys Phe Thr Glu Val Pro Gly Glu Phe Pro Phe Thr Arg Gly Thr Thr70 75 80 85gtt gat ggt gaa cgc gtt ggt tgg ggt gtt act gag act ttc gga cat 403Val Asp Gly Glu Arg Val Gly Trp Gly Val Thr Glu Thr Phe Gly His
90 95 100gac agc ccg aag aat atc aac gct gcg gtg ctg aat gct ctg aat tct 451Asp Ser Pro Lys Asn Ile Asn Ala Ala Val Leu Asn Ala Leu Asn Ser
105 110 115ggc acc acc aca ttg ggt ttt gag ttc tct gag gaa ttc acg gca gct 499Gly Thr Thr Thr Leu Gly Phe Glu Phe Ser Glu Glu Phe Thr Ala Ala
120 125 130gat ctt aaa gtt gct ctc gaa ggc gtg tat ctc aac atg gct ccg ttg 547Asp Leu Lys Val Ala Leu Glu Gly Val Tyr Leu Asn Met Ala Pro Leu
135 140 145ctg att cat gcg ggt gga tcc acg tca gag gtt gca gcg gcg ttg tat 595Leu Ile His Ala Gly Gly Ser Thr Ser Glu Val Ala Ala Ala Leu Tyr150 155 160 165acg ttg gcg gag gaa gcc gga acg ttt ttt gct gcg ttg acc ttg ggt 643Thr Leu Ala Glu Glu Ala Gly Thr Phe Phe Ala Ala Leu Thr Leu Gly
170 175 180tct cgt cct ttg acg gcg cag gtt gat ggt tcg cac agt gac acc att 691Ser Arg Pro Leu Thr Ala Gln Val Asp Gly Ser His Ser Asp Thr Ile
185 190 195gaa gaa gca gtt cag ttg gca gtg aat gct tcc aag cgt gcg aat gtg 739Glu Glu Ala Val Gln Leu Ala Val Asn Ala Ser Lys Arg Ala Asn Val
200 205 210cgc gct atc ttg gtg gat ggt tcc agt ttt tcc aac cag ggc gcg tcg 787Arg Ala Ile Leu Val Asp Gly Ser Ser Phe Ser Asn Gln Gly Ala Ser
215 220 225gat gct caa gaa att ggt cta agt atc gcc gcc ggt gtg gat tat gtc 835Asp Ala Gln Glu Ile Gly Leu Ser Ile Ala Ala Gly Val Asp Tyr Val230 235 240 245cgt cgc ttg gtc gat gca ggc ctt tcc acg gaa gct gca ctt aag cag 883Arg Arg Leu Val Asp Ala Gly Leu Ser Thr Glu Ala Ala Leu Lys Gln
250 255 260gtg gcg ttc cgt ttt gcg gtc acc gat gag cag ttc gcg cag att tct 931Val Ala Phe Arg Phe Ala Val Thr Asp Glu Gln Phe Ala Gln Ile Ser
265 270 275aag ctg cgt gtg gct cga cgt ctg tgg gcc agg gtg tgt gag gtg ctt 979Lys Leu Arg Val Ala Arg Arg Leu Trp Ala Arg Val Cys Glu Val Leu
280 285 290ggt ttt cca gag ctg gcc gta gca cca cag cat gcg gtg act gca cga 1027Gly Phe Pro Glu Leu Ala Val Ala Pro Gln His Ala Val Thr Ala Arg
295 300 305gcg atg ttt agc cag cgt gat ccg tgg gtg aat atg ctg cgc agt act 1075Ala Met Phe Ser Gln Arg Asp Pro Trp Val Asn Met Leu Arg Ser Thr310 315 320 325gtt gca gct ttc gct gca ggc gtc ggt gga gca acc gat gtg gag gtt 1123Val Ala Ala Phe Ala Ala Gly Val Gly Gly Ala Thr Asp Val Glu Val
330 335 340cgt act ttt gat gat gcg atc cca gat gga gtt cct gga gtg tcg agg 1171Arg Thr Phe Asp Asp Ala Ile Pro Asp Gly Val Pro Gly Val Ser Arg
345 350 355aat ttc gct cac cgc atc gcg cgc aat act aat ttg ttg ttg cta gaa 1219Asn Phe Ala His Arg Ile Ala Arg Asn Thr Asn Leu Leu Leu Leu Glu
360 365 370gag tca cat ctt ggt cac gtg gtt gat cct gct ggt gga tca tat ttc 1267Glu Ser His Leu Gly His Val Val Asp Pro Ala Gly Gly Ser Tyr Phe
375 380 385gtg gag agc ttc acc gat gat cta gcg gag aag gcg tgg gct gtg ttc 1315Val Glu Ser Phe Thr Asp Asp Leu Ala Glu Lys Ala Trp Ala Val Phe390 395 400 405agt ggc atc gaa gct gag ggc gga tac agt gca gct tgt gca tcc ggc 1363Ser Gly Ile Glu Ala Glu Gly Gly Tyr Ser Ala Ala Cys Ala Ser Gly
410 415 420acg gtg act gcc atg ctt gat cag acg tgg gag cag act cgc gct gat 1411Thr Val Thr Ala Met Leu Asp Gln Thr Trp Glu Gln Thr Arg Ala Asp
425 430 435gtg gcg tcg aga aag aag aag ctc act gga att aat gag ttc ccg aac 1459Val Ala Ser Arg Lys Lys Lys Leu Thr Gly Ile Asn Glu Phe Pro Asn
440 445 450ttg gcg gag tct ccg ctg cca gct gat cgt cgg gta gaa cct gca ggt 1507Leu Ala Glu Ser Pro Leu Pro Ala Asp Arg Arg Val Glu Pro Ala Gly
455 460 465gtg cgt cga tgg gca gcg gat ttt gaa gcg ctg cgc aat cgt tcg gat 1555Val Arg Arg Trp Ala Ala Asp Phe Glu Ala Leu Arg Asn Arg Ser Asp470 475 480 485gct ttc ttg gaa aag aac ggc gcg agg cca cag atc acg atg att cct 1603Ala Phe Leu Glu Lys Asn Gly Ala Arg Pro Gln Ile Thr Met Ile Pro
490 495 500ctg gga ccg ttg tcc aag cac aat att cgc act ggt ttt act tcc aac 1651Leu Gly Pro Leu Ser Lys His Asn Ile Arg Thr Gly Phe Thr Ser Asn
505 510 515ctg ttg gct tcc ggt ggc att gaa gca atc aac ccg ggt caa ctt gtt 1699Leu Leu Ala Ser Gly Gly Ile Glu Ala Ile Asn Pro Gly Gln Leu Val
520 525 530ccc ggc act gac gct ttt gca gaa gct gca cag gcc gca ggc att gta 1747Pro Gly Thr Asp Ala Phe Ala Glu Ala Ala Gln Ala Ala Gly Ile Val
535 540 545gtg gtg tgt gga acg gac caa gag tat gcc gaa acg ggg gag gga gcc 1795Val Val Cys Gly Thr Asp Gln Glu Tyr Ala Glu Thr Gly Glu Gly Ala550 555 560 565gtc gaa aag ctc cgc gaa gcg ggc gtt gag cgc atc ctg ctt gct ggc 1843Val Glu Lys Leu Arg Glu Ala Gly Val Glu Arg Ile Leu Leu Ala Gly
570 575 580gcg ccg aag agc ttt gag ggc agc gcg cat gcg ccc gat ggt tat ttg 1891Ala Pro Lys Ser Phe Glu Gly Ser Ala His Ala Pro Asp Gly Tyr Leu
585 590 595aac atg aca att gat gcc gcg gcg acg ctg gct gac ctg cta gat gct 1939Asn Met Thr Ile Asp Ala Ala Ala Thr Leu Ala Asp Leu Leu Asp Ala
600 605 610ttg gga gct taaatcatga cgtcgatccc taa 1971Leu Gly Ala
615<210>632<211>616<212>PRT<213>谷氨酸棒杆菌<400>632Leu Thr Asp Leu Thr Lys Thr Ala Val Pro Glu Glu Leu Ser Glu Asn1 5 10 15Leu Glu Thr Trp Tyr Lys Ala Val Ala Gly Val Phe Ala Arg Thr Gln
20 25 30Lys Lys Asp Ile Gly Asp Ile Ala Val Asp Val Trp Lys Lys Leu Ile
35 40 45Val Thr Thr Pro Asp Gly Val Asp Ile Asn Pro Leu Tyr Thr Arg Ala
50 55 60Asp Glu Ser Gln Arg Lys Phe Thr Glu Val Pro Gly Glu Phe Pro Phe65 70 75 80Thr Arg Gly Thr Thr Val Asp Gly Glu Arg Val Gly Trp Gly Val Thr
85 90 95Glu Thr Phe Gly His Asp Ser Pro Lys Asn Ile Asn Ala Ala Val Leu
100 105 110Asn Ala Leu Asn Ser Gly Thr Thr Thr Leu Gly Phe Glu Phe Ser Glu
115 120 125Glu Phe Thr Ala Ala Asp Leu Lys Val Ala Leu Glu Gly Val Tyr Leu
130 135 140Asn Met Ala Pro Leu Leu Ile His Ala Gly Gly Ser Thr Ser Glu Val145 150 155 160Ala Ala Ala Leu Tyr Thr Leu Ala Glu Glu Ala Gly Thr Phe Phe Ala
165 170 175Ala Leu Thr Leu Gly Ser Arg Pro Leu Thr Ala Gln Val Asp Gly Ser
180 185 190His Ser Asp Thr Ile Glu Glu Ala Val Gln Leu Ala Val Asn Ala Ser
195 200 205Lys Arg Ala Asn Val Arg Ala Ile Leu Val Asp Gly Ser Ser Phe Ser
210 215 220Asn Gln Gly Ala Ser Asp Ala Gln Glu Ile Gly Leu Ser Ile Ala Ala225 230 235 240Gly Val Asp Tyr Val Arg Arg Leu Val Asp Ala Gly Leu Ser Thr Glu
245 250 255Ala Ala Leu Lys Gln Val Ala Phe Arg Phe Ala Val Thr Asp Glu Gln
260 265 270Phe Ala Gln Ile Ser Lys Leu Arg Val Ala Arg Arg Leu Trp Ala Arg
275 280 285Val Cys Glu Val Leu Gly Phe Pro Glu Leu Ala Val Ala Pro Gln His
290 295 300Ala Val Thr Ala Arg Ala Met Phe Ser Gln Arg Asp Pro Trp Val Asn305 310 315 320Met Leu Arg Ser Thr Val Ala Ala Phe Ala Ala Gly Val Gly Gly Ala
325 330 335Thr Asp Val Glu Val Arg Thr Phe Asp Asp Ala Ile Pro Asp Gly Val
340 345 350Pro Gly Val Ser Arg Asn Phe Ala His Arg Ile Ala Arg Asn Thr Asn
355 360 365Leu Leu Leu Leu Glu Glu Ser His Leu Gly His Val Val Asp Pro Ala
370 375 380Gly Gly Ser Tyr Phe Val Glu Ser Phe Thr Asp Asp Leu Ala Glu Lys385 390 395 400Ala Trp Ala Val Phe Ser Gly Ile Glu Ala Glu Gly Gly Tyr Ser Ala
405 410 415Ala Cys Ala Ser Gly Thr Val Thr Ala Met Leu Asp Gln Thr Trp Glu
420 425 430Gln Thr Arg Ala Asp Val Ala Ser Arg Lys Lys Lys Leu Thr Gly Ile
435 440 445Asn Glu Phe Pro Asn Leu Ala Glu Ser Pro Leu Pro Ala Asp Arg Arg
450 455 460Val Glu Pro Ala Gly Val Arg Arg Trp Ala Ala Asp Phe Glu Ala Leu465 470 475 480Arg Asn Arg Ser Asp Ala Phe Leu Glu Lys Asn Gly Ala Arg Pro Gln
485 490 495Ile Thr Met Ile Pro Leu Gly Pro Leu Ser Lys His Asn Ile Arg Thr
500 505 510Gly Phe Thr Ser Asn Leu Leu Ala Ser Gly Gly Ile Glu Ala Ile Asn
515 520 525Pro Gly Gln Leu Val Pro Gly Thr Asp Ala Phe Ala Glu Ala Ala Gln
530 535 540Ala Ala Gly Ile Val Val Val Cys Gly Thr Asp Gln Glu Tyr Ala Glu545 550 555 560Thr Gly Glu Gly Ala Val Glu Lys Leu Arg Glu Ala Gly Val Glu Arg
565 570 575Ile Leu Leu Ala Gly Ala Pro Lys Ser Phe Glu Gly Ser Ala His Ala
580 585 590Pro Asp Gly Tyr Leu Asn Met Thr Ile Asp Ala Ala Ala Thr Leu Ala
595 600 605Asp Leu Leu Asp Ala Leu Gly Ala
610 615<210>633<211>777<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(754)<223>RXN00317<400>633caacggttac atcactcacg cattaactcc cctggttggt accgcatgtt cgtttcccaa 60gtctagccaa gccttgaaaa attctggcaa ggttaatggt gtg act acg cct tct 115
Val Thr Thr Pro Ser
1 5aag aaa act ctg ctc ttt gat ctc gac gga acc ctc gtc gat tct ttc 163Lys Lys Thr Leu Leu Phe Asp Leu Asp Gly Thr Leu Val Asp Ser Phe
10 15 20ccc ggt atc cgc act tca ttc ctt cac acc ctg cac gaa aag aac tgg 211Pro Gly Ile Arg Thr Ser Phe Leu His Thr Leu His Glu Lys Asn Trp
25 30 35gaa atc ccc tct gag gaa cgc atc tcg caa gtt cca gga cct ccc atg 259Glu Ile Pro Ser Glu Glu Arg Ile Ser Gln Val Pro Gly Pro Pro Met
40 45 50gaa tgg acg ttc cag gat ttg ggc atg act cca gag cag gca caa gac 307Glu Trp Thr Phe Gln Asp Leu Gly Met Thr Pro Glu Gln Ala Gln Asp
55 60 65gct ctg cag acc tac ctt gag cat tac ggc cag gtg ggt tgg gat ctt 355Ala Leu Gln Thr Tyr Leu Glu His Tyr Gly Gln Val Gly Trp Asp Leu70 75 80 85tcc gaa gca ttc ccc ggc atg cga gat ttg ctg atc cgc ttg aaa tac 403Ser Glu Ala Phe Pro Gly Met Arg Asp Leu Leu Ile Arg Leu Lys Tyr
90 95 100gaa ggt ttc cgt ctg tgc acc gcc acc tcc aag ggc gag ttc ttt gcg 451Glu Gly Phe Arg Leu Cys Thr Ala Thr Ser Lys Gly Glu Phe Phe Ala
105 110 115gag aag gta ctt cgc aaa ttc gag atg ttc gat ctc ttc gaa ttc atg 499Glu Lys Val Leu Arg Lys Phe Glu Met Phe Asp Leu Phe Glu Phe Met
120 125 130ggt gcc gcc acc gac agc ggc aac cga cgc agc aaa tct gcc gtg atc 547Gly Ala Ala Thr Asp Ser Gly Asn Arg Arg Ser Lys Ser Ala Val Ile
135 140 145aaa cat gtc ctc gac agc gtt ggg ttg gac gaa cca aat gat att ttg 595Lys His Val Leu Asp Ser Val Gly Leu Asp Glu Pro Asn Asp Ile Leu150 155 160 165atg att ggt gat cga tca cac gat att gaa ggt tcg agt gaa ttc ggc 643Met Ile Gly Asp Arg Ser His Asp Ile Glu Gly Ser Ser Glu Phe Gly
170 175 180atc gat tgt gtt gcc gta acc tgg ggc tac ggc agc aaa act gaa tgg 691Ile Asp Cys Val Ala Val Thr Trp Gly Tyr Gly Ser Lys Thr Glu Trp
185 190 195gac gct gcc cgc tac acc gtg agc acc gca gaa gaa tta gaa agg atc 739Asp Ala Ala Arg Tyr Thr Val Ser Thr Ala Glu Glu Leu Glu Arg Ile
200 205 210atc cat gac tgg gcc taaaacttcg ctacctgtgg aaa 777Ile His Asp Trp Ala
215<210>634<211>218<212>PRT<213>谷氨酸棒杆菌<400>634Val Thr Thr Pro Ser Lys Lys Thr Leu Leu Phe Asp Leu Asp Gly Thr1 5 10 15Leu Val Asp Ser Phe Pro Gly Ile Arg Thr Ser Phe Leu His Thr Leu
20 25 30His Glu Lys Asn Trp Glu Ile Pro Ser Glu Glu Arg Ile Ser Gln Val
35 40 45Pro Gly Pro Pro Met Glu Trp Thr Phe Gln Asp Leu Gly Met Thr Pro
50 55 60Glu Gln Ala Gln Asp Ala Leu Gln Thr Tyr Leu Glu His Tyr Gly Gln65 70 75 80Val Gly Trp Asp Leu Ser Glu Ala Phe Pro Gly Met Arg Asp Leu Leu
85 90 95Ile Arg Leu Lys Tyr Glu Gly Phe Arg Leu Cys Thr Ala Thr Ser Lys
100 105 110Gly Glu Phe Phe Ala Glu Lys Val Leu Arg Lys Phe Glu Met Phe Asp
115 120 125Leu Phe Glu Phe Met Gly Ala Ala Thr Asp Ser Gly Asn Arg Arg Ser
130 135 140Lys Ser Ala Val Ile Lys His Val Leu Asp Ser Val Gly Leu Asp Glu145 150 155 160Pro Asn Asp Ile Leu Met Ile Gly Asp Arg Ser His Asp Ile Glu Gly
165 170 175Ser Ser Glu Phe Gly Ile Asp Cys Val Ala Val Thr Trp Gly Tyr Gly
180 185 190Ser Lys Thr Glu Trp Asp Ala Ala Arg Tyr Thr Val Ser Thr Ala Glu
195 200 205Glu Leu Glu Arg Ile Ile His Asp Trp Ala
210 215<210>635<211>439<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(439)<223>FRXA00317<400>635caacggttac atcactcacg cattacctcc cctggttggt accgcatgtt cgtttcccaa 60gtcaagccaa gccttgaaaa attctggcaa ggttaatggt gtg act acg cct tct 115
Val Thr Thr Pro Ser
1 5aag aaa act ctg ctc ttt gat ctc gac gga acc ctc gtc gat tct ttc 163Lys Lys Thr Leu Leu Phe Asp Leu Asp Gly Thr Leu Val Asp Ser Phe
10 15 20ccc ggt atc cgc act tca ttc ctt cac acc ctg cac gaa aag aac tgg 211Pro Gly Ile Arg Thr Ser Phe Leu His Thr Leu His Glu Lys Asn Trp
25 30 35gaa atc ccc tct gag gaa cgc atc tcg caa gtt cca gga cct ccc atg 259Glu Ile Pro Ser Glu Glu Arg Ile Ser Gln Val Pro Gly Pro Pro Met
40 45 50gaa tgg acg ttc cag gat ttg ggc atg act cca gag cag gca caa gac 307Glu Trp Thr Phe Gln Asp Leu Gly Met Thr Pro Glu Gln Ala Gln Asp
55 60 65gct ctg cag acc tac ctt gag cat tac ggc cag gtg ggt tgg gat ctt 355Ala Leu Gln Thr Tyr Leu Glu His Tyr Gly Gln Val Gly Trp Asp Leu70 75 80 85tcc gaa gca ttc ccc ggc atg cga gat ttg ctg atc ccc ttg ata tac 403Ser Glu Ala Phe Pro Gly Met Arg Asp Leu Leu Ile Pro Leu Ile Tyr
90 95 100gaa ggt ttt cgt ctg tgc acc gcc acc ttt caa ggg 439Glu Gly Phe Arg Leu Cys Thr Ala Thr Phe Gln Gly
105 110<210>636<211>113<212>PRT<213>谷氨酸棒杆菌<400>636Val Thr Thr Pro Ser Lys Lys Thr Leu Leu Phe Asp Leu Asp Gly Thr1 5 10 15Leu Val Asp Ser Phe Pro Gly Ile Arg Thr Ser Phe Leu His Thr Leu
20 25 30His Glu Lys Asn Trp Glu Ile Pro Ser Glu Glu Arg Ile Ser Gln Val
35 40 45Pro Gly Pro Pro Met Glu Trp Thr Phe Gln Asp Leu Gly Met Thr Pro
50 55 60Glu Gln Ala Gln Asp Ala Leu Gln Thr Tyr Leu Glu His Tyr Gly Gln65 70 75 80Val Gly Trp Asp Leu Ser Glu Ala Phe Pro Gly Met Arg Asp Leu Leu
85 90 95Ile Pro Leu Ile Tyr Glu Gly Phe Arg Leu Cys Thr Ala Thr Phe Gln
100 105 110Gly<210>637<211>816<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(793)<223>RXA02196<400>637tctaccaccc agaggcaaag tactttaacg tctaacacct ttgagaggga aaactttccc 60gcacattgca gatcgtgcca ctttaactaa ggttgacggc atg att aag gcg att 115
Met Ile Lys Ala Ile
1 5ttc tgg gac atg gac ggc acg atg gtg gac tct gag cca cag tgg ggc 163Phe Trp Asp Met Asp Gly Thr Met Val Asp Ser Glu Pro Gln Trp Gly
10 15 20att gct acc tac gag ctc agc gaa gcc atg ggc cgc cgc ctc acc ccg 211Ile Ala Thr Tyr Glu Leu Ser Glu Ala Met Gly Arg Arg Leu Thr Pro
25 30 35gag ctc cgg gaa ctc acc gtc ggc tcg agc ctg ccg cgc acc atg cgc 259Glu Leu Arg Glu Leu Thr Val Gly Ser Ser Leu Pro Arg Thr Met Arg
40 45 50tta tgc gca gag cac gca ggc att aca ttg agc gac gcg gac tac gag 307Leu Cys Ala Glu His Ala Gly Ile Thr Leu Ser Asp Ala Asp Tyr Glu
55 60 65cgc tac cgg gct ggc atg ttc gcc cgg gtc cat gag ctt ttc gac gaa 355Arg Tyr Arg Ala Gly Met Phe Ala Arg Val His Glu Leu Phe Asp Glu70 75 80 85tcc ctc gtc cca aat cca ggc gtc acc gaa ctc ctg aca gag ttg aag 403Ser Leu Val Pro Asn Pro Gly Val Thr Glu Leu Leu Thr Glu Leu Lys
90 95 100gcc ctc gag atc ccc atg ttg gtc acc acc aac aca gag cgc gat ctc 451Ala Leu Glu Ile Pro Met Leu Val Thr Thr Asn Thr Glu Arg Asp Leu
105 110 115gcg acc cgt tca gtc gca gcc gtg gga aat gag ttc ttc atc ggt tct 499Ala Thr Arg Ser Val Ala Ala Val Gly Asn Glu Phe Phe Ile Gly Ser
120 125 130atc gct ggt gat gaa gtc cca aca gca aag cca gcc ccc gac atg tac 547Ile Ala Gly Asp Glu Val Pro Thr Ala Lys Pro Ala Pro Asp Met Tyr
135 140 145ctc gaa gca gca cga cgt gtg ggc ttt gac cca tca gag tgc ctc gtg 595Leu Glu Ala Ala Arg Arg Val Gly Phe Asp Pro Ser Glu Cys Leu Val150 155 160 165ttc gaa gat tcc tac aac ggc atg ctg ggc gct gtt act gca ggt tgc 643Phe Glu Asp Ser Tyr Asn Gly Met Leu Gly Ala Val Thr Ala Gly Cys
170 175 180cgc gtc att ggt ctg cac cca gaa gaa gtc caa gcg cca gaa ggt gta 691Arg Val Ile Gly Leu His Pro Glu Glu Val Gln Ala Pro Glu Gly Val
185 190 195gtg cct ttg cgt tcc ctc cac ggt aaa aac tct ttc gaa ggt gtc acc 739Val Pro Leu Arg Ser Leu His Gly Lys Asn Ser Phe Glu Gly Val Thr
200 205 210gct gag atg gtc act gcc tgg tac cac cag atc gag ccg gca ggt gtc 787Ala Glu Met Val Thr Ala Trp Tyr His Gln Ile Glu Pro Ala Gly Val
215 220 225gca aaa taaaaccagg tgggggagtg aaa 816Ala Lys230<210>638<211>231<212>PRT<213>谷氨酸棒杆菌<400>638Met Ile Lys Ala Ile Phe Trp Asp Met Asp Gly Thr Met Val Asp Ser1 5 10 15Glu Pro Gln Trp Gly Ile Ala Thr Tyr Glu Leu Ser Glu Ala Met Gly
20 25 30Arg Arg Leu Thr Pro Glu Leu Arg Glu Leu Thr Val Gly Ser Ser Leu
35 40 45Pro Arg Thr Met Arg Leu Cys Ala Glu His Ala Gly Ile Thr Leu Ser
50 55 60Asp Ala Asp Tyr Glu Arg Tyr Arg Ala Gly Met Phe Ala Arg Val His65 70 75 80Glu Leu Phe Asp Glu Ser Leu Val Pro Asn Pro Gly Val Thr Glu Leu
85 90 95Leu Thr Glu Leu Lys Ala Leu Glu Ile Pro Met Leu Val Thr Thr Asn
100 105 110Thr Glu Arg Asp Leu Ala Thr Arg Ser Val Ala Ala Val Gly Asn Glu
115 120 125Phe Phe Ile Gly Ser Ile Ala Gly Asp Glu Val Pro Thr Ala Lys Pro
130 135 140Ala Pro Asp Met Tyr Leu Glu Ala Ala Arg Arg Val Gly Phe Asp Pro145 150 155 160Ser Glu Cys Leu Val Phe Glu Asp Ser Tyr Asn Gly Met Leu Gly Ala
165 170 175Val Thr Ala Gly Cys Arg Val Ile Gly Leu His Pro Glu Glu Val Gln
180 185 190Ala Pro Glu Gly Val Val Pro Leu Arg Ser Leu His Gly Lys Asn Ser
195 200 205Phe Glu Gly Val Thr Ala Glu Met Val Thr Ala Trp Tyr His Gln Ile
210 215 220Glu Pro Ala Gly Val Ala Lys225 230<210>639<211>531<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(508)<223>RXN02461<400>639agaaaatctc aaggcaaaaa acaagccacc ccaatctgtg cgacaatcaa accacagact 60acgactatta tgtcacgaag aaaccaaaga aagggaaata atg cgc gga cta att 115
Met Arg Gly Leu Ile
1 5gtt gac tac gct gga gta cta gac gga acc gat gag gac cag cgt cgc 163Val Asp Tyr Ala Gly Val Leu Asp Gly Thr Asp Glu Asp Gln Arg Arg
10 15 20tgg cgc aac ctg ctc gcc gca gca aag aaa aat ggc gtc gga acc gtg 211Trp Arg Asn Leu Leu Ala Ala Ala Lys Lys Asn Gly Val Gly Thr Val
25 30 35atc ctc agc aac gat cca ggt ggg ctc ggc gca gcg ccg atc cgg gaa 259Ile Leu Ser Asn Asp Pro Gly Gly Leu Gly Ala Ala Pro Ile Arg Glu
40 45 50ctc gaa aca aac ggg gta gtc gat aag gtg ctg ctg tcg gga gaa ctt 307Leu Glu Thr Asn Gly Val Val Asp Lys Val Leu Leu Ser Gly Glu Leu
55 60 65ggc gtc gaa aag cca gag gaa gca gct ttc cag gcc gcc gca gac gcc 355Gly Val Glu Lys Pro Glu Glu Ala Ala Phe Gln Ala Ala Ala Asp Ala70 75 80 85atc gac ctg ccc atg cgt gac tgc gtg ctt gtc gac gac tcg atc ctc 403Ile Asp Leu Pro Met Arg Asp Cys Val Leu Val Asp Asp Ser Ile Leu
90 95 100aac gtg cgc ggc gcc gtc gaa gcc gga ctc gta ggc gtc tac tac cag 451Asn Val Arg Gly Ala Val Glu Ala Gly Leu Val Gly Val Tyr Tyr Gln
105 110 115caa ttt gac cgt gca gtc gtc gaa atc gtc gga ctg ttc ggg cta gaa 499Gln Phe Asp Arg Ala Val Val Glu Ile Val Gly Leu Phe Gly Leu Glu
120 125 130gga gaa ttc taatcttgcg cgtctacatc cca 531Gly Glu Phe
135<210>640<211>136<212>PRT<213>谷氨酸棒杆菌<400>640Met Arg Gly Leu Ile Val Asp Tyr Ala Gly Val Leu Asp Gly Thr Asp1 5 10 15Glu Asp Gln Arg Arg Trp Arg Asn Leu Leu Ala Ala Ala Lys Lys Asn
20 25 30Gly Val Gly Thr Val Ile Leu Ser Asn Asp Pro Gly Gly Leu Gly Ala
35 40 45Ala Pro Ile Arg Glu Leu Glu Thr Asn Gly Val Val Asp Lys Val Leu
50 55 60Leu Ser Gly Glu Leu Gly Val Glu Lys Pro Glu Glu Ala Ala Phe Gln65 70 75 80Ala Ala Ala Asp Ala Ile Asp Leu Pro Met Arg Asp Cys Val Leu Val
85 90 95Asp Asp Ser Ile Leu Asn Val Arg Gly Ala Val Glu Ala Gly Leu Val
100 105 110Gly Val Tyr Tyr Gln Gln Phe Asp Arg Ala Val Val Glu Ile Val Gly
115 120 125Leu Phe Gly Leu Glu Gly Glu Phe
130 135<210>641<211>1662<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1639)<223>RXN01744<400>641tcttattggt tcttcgtttt gtatcgataa atacaatcgg tttcctggct taataaggct 60gttcctgtca acctgcaatg gaagaggaag tgtacctagc gtg gat gtc gtc gac 115
Val Asp Val Val Asp
1 5atc gca cgg tgg caa ttc gga att acc acc gtc tat cac ttc att ttt 163Ile Ala Arg Trp Gln Phe Gly Ile Thr Thr Val Tyr His Phe Ile Phe
10 15 20gtc cca ctg acc att ggc tta gcg ccg ctg gtc gca atc atg caa acg 211Val Pro Leu Thr Ile Gly Leu Ala Pro Leu Val Ala Ile Met Gln Thr
25 30 35ttt tgg caa gtt acc ggc aaa gag cac tgg tat cgg gcc aca aga ttt 259Phe Trp Gln Val Thr Gly Lys Glu His Trp Tyr Arg Ala Thr Arg Phe
40 45 50ttt ggc act gtg ctg ctc atc aac ttc gcg gtt ggt gta gca acg ggc 307Phe Gly Thr Val Leu Leu Ile Asn Phe Ala Val Gly Val Ala Thr Gly
55 60 65att gtg cag gag ttc cag ttc ggt atg aac tgg tcg gaa tat tcg cgt 355Ile Val Gln Glu Phe Gln Phe Gly Met Asn Trp Ser Glu Tyr Ser Arg70 75 80 85ttc gtc ggt gat gtt ttc ggc gga ccg ctg gct ttg gag ggt ctt atc 403Phe Val Gly Asp Val Phe Gly Gly Pro Leu Ala Leu Glu Gly Leu Ile
90 95 100gcg ttc ttc ctt gag tct gta ttc ctg gga ctg tgg att ttc gga tgg 451Ala Phe Phe Leu Glu Ser Val Phe Leu Gly Leu Trp Ile Phe Gly Trp
105 110 115ggg aag att cct ggt tgg ttg cac act gca tcc att tgg atc gtt gct 499Gly Lys Ile Pro Gly Trp Leu His Thr Ala Ser Ile Trp Ile Val Ala
120 125 130att gcg acg aat att tct gcc tat ttc atc atc gtg gcc aac tcg ttt 547Ile Ala Thr Asn Ile Ser Ala Tyr Phe Ile Ile Val Ala Asn Ser Phe
135 140 145atg cag cat ccg gtg ggt gct gag tat aac cct gag act ggt cgt gcg 595Met Gln His Pro Val Gly Ala Glu Tyr Asn Pro Glu Thr Gly Arg Ala150 155 160 165gag ctt act gat ttt tgg gct ctt ctc aca aac tcc acc gcg ctg gct 643Glu Leu Thr Asp Phe Trp Ala Leu Leu Thr Asn Ser Thr Ala Leu Ala
170 175 180gcg ttc ccg cat gct gtt gcc ggt ggt ttt tta aca gct gga act ttc 691Ala Phe Pro His Ala Val Ala Gly Gly Phe Leu Thr Ala Gly Thr Phe
185 190 195gtt ctc gga att tcg ggt tgg tgg att att cgt gcg cac cgt cag gcc 739Val Leu Gly Ile Ser Gly Trp Trp Ile Ile Arg Ala His Arg Gln Ala
200 205 210aag aag gct gag tcg gaa atc gag tcg aag cat tcg atg cac agg ccc 787Lys Lys Ala Glu Ser Glu Ile Glu Ser Lys His Ser Met His Arg Pro
215 220 225gcg ttg tgg gtt ggt tgg tgg acc aca gtt gtc tct tcc gtg gcg ctg 835Ala Leu Trp Val Gly Trp Trp Thr Thr Val Val Ser Ser Val Ala Leu230 235 240 245ttc atc act ggc gat atc cag gcg aag ctc atg ttc gtg caa cag cca 883Phe Ile Thr Gly Asp Ile Gln Ala Lys Leu Met Phe Val Gln Gln Pro
250 255 260atg aag atg gcg tcg gcg gaa tcc ttg tgt gaa acc gcc aca gat cca 931Met Lys Met Ala Ser Ala Glu Ser Leu Cys Glu Thr Ala Thr Asp Pro
265 270 275aac ttc tcc att ctg aca att ggt acg cac aac aac tgc gat acg gta 979Asn Phe Ser Ile Leu Thr Ile Gly Thr His Asn Asn Cys Asp Thr Val
280 285 290acc cac ctg atc gat gtt ccg ttt gtg ctt cca ttc ttg gct gaa gga 1027Thr His Leu Ile Asp Val Pro Phe Val Leu Pro Phe Leu Ala Glu Gly
295 300 305aaa ttc acc ggt gtg act ttg cag ggt gta aac cag ctc caa gct gca 1075Lys Phe Thr Gly Val Thr Leu Gln Gly Val Asn Gln Leu Gln Ala Ala310 315 320 325gcg gag caa gca tac ggt cct ggc aac tac tcc cct aac ttg ttt gtc 1123Ala Glu Gln Ala Tyr Gly Pro Gly Asn Tyr Ser Pro Asn Leu Phe Val
330 335 340acc tac tgg tca ttc cgc gca atg atc ggc ctg atg ctt ggt tct ttg 1171Thr Tyr Trp Ser Phe Arg Ala Met Ile Gly Leu Met Leu Gly Ser Leu
345 350 355gct atc gct gcg att gcg tgg ctg ttg ctg cgt aag aag cgc aca cca 1219Ala Ile Ala Ala Ile Ala Trp Leu Leu Leu Arg Lys Lys Arg Thr Pro
360 365 370act gga aag att gct cgt ctg ttc cag atc ggc agc ctc att gct atc 1267Thr Gly Lys Ile Ala Arg Leu Phe Gln Ile Gly Ser Leu Ile Ala Ile
375 380 385ccg ttc cca ttc ttg gcc aac tct gct ggt tgg atc ttc acc gag atg 1315Pro Phe Pro Phe Leu Ala Asn Ser Ala Gly Trp Ile Phe Thr Glu Met390 395 400 405ggc cgc cag cct tgg gtg gtg cac ccg aac cct gaa tct gcc ggc gat 1363Gly Arg Gln Pro Trp Val Val His Pro Asn Pro Glu Ser Ala Gly Asp
410 415 420gcc cga aca gag atg atc cgg atg act gtt gat atg ggt gta tct gat 1411Ala Arg Thr Glu Met Ile Arg Met Thr Val Asp Met Gly Val Ser Asp
425 430 435cat gcg cca tgg caa gtc tgg ctg act ctc att ggc ttc acg att ctc 1459His Ala Pro Trp Gln Val Trp Leu Thr Leu Ile Gly Phe Thr Ile Leu
440 445 450tat ctc att ttg ttc gtg gtg tgg gtg tgg ctg att cgc cgc gca gtt 1507Tyr Leu Ile Leu Phe Val Val Trp Val Trp Leu Ile Arg Arg Ala Val
455 460 465ctg atc gga cca cca gag gaa ggc gct cca tcc gtg gag gca aag act 1555Leu Ile Gly Pro Pro Glu Glu Gly Ala Pro Ser Val Glu Ala Lys Thr470 475 480 485gga ccg gca acc ccg att ggt tca gat atg ccc atg aca ccg ctg caa 1603Gly Pro Ala Thr Pro Ile Gly Ser Asp Met Pro Met Thr Pro Leu Gln
490 495 500ttt act gcc gct gcc cca acc aca ggt gaa aag gaa taaccatgga 1649Phe Thr Ala Ala Ala Pro Thr Thr Gly Glu Lys Glu
505 510tctcaatacc ttt 1662<210>642<211>513<212>PRT<213>谷氨酸棒杆菌<400>642Val Asp Val Val Asp Ile Ala Arg Trp Gln Phe Gly Ile Thr Thr Val1 5 10 15Tyr His Phe Ile Phe Val Pro Leu Thr Ile Gly Leu Ala Pro Leu Val
20 25 30Ala Ile Met Gln Thr Phe Trp Gln Val Thr Gly Lys Glu His Trp Tyr
35 40 45Arg Ala Thr Arg Phe Phe Gly Thr Val Leu Leu Ile Asn Phe Ala Val
50 55 60Gly Val Ala Thr Gly Ile Val Gln Glu Phe Gln Phe Gly Met Asn Trp65 70 75 80Ser Glu Tyr Ser Arg Phe Val Gly Asp Val Phe Gly Gly Pro Leu Ala
85 90 95Leu Glu Gly Leu Ile Ala Phe Phe Leu Glu Ser Val Phe Leu Gly Leu
100 105 110Trp Ile Phe Gly Trp Gly Lys Ile Pro Gly Trp Leu His Thr Ala Ser
115 120 125Ile Trp Ile Val Ala Ile Ala Thr Asn Ile Ser Ala Tyr Phe Ile Ile
130 135 140Val Ala Asn Ser Phe Met Gln His Pro Val Gly Ala Glu Tyr Asn Pro145 150 155 160Glu Thr Gly Arg Ala Glu Leu Thr Asp Phe Trp Ala Leu Leu Thr Asn
165 170 175Ser Thr Ala Leu Ala Ala Phe Pro His Ala Val Ala Gly Gly Phe Leu
180 185 190Thr Ala Gly Thr Phe Val Leu Gly Ile Ser Gly Trp Trp Ile Ile Arg
195 200 205Ala His Arg Gln Ala Lys Lys Ala Glu Ser Glu Ile Glu Ser Lys His
210 215 220Ser Met His Arg Pro Ala Leu Trp Val Gly Trp Trp Thr Thr Val Val225 230 235 240Ser Ser Val Ala Leu Phe Ile Thr Gly Asp Ile Gln Ala Lys Leu Met
245 250 255Phe Val Gln Gln Pro Met Lys Met Ala Ser Ala Glu Ser Leu Cys Glu
260 265 270Thr Ala Thr Asp Pro Asn Phe Ser Ile Leu Thr Ile Gly Thr His Asn
275 280 285Asn Cys Asp Thr Val Thr His Leu Ile Asp Val Pro Phe Val Leu Pro
290 295 300Phe Leu Ala Glu Gly Lys Phe Thr Gly Val Thr Leu Gln Gly Val Asn305 310 315 320Gln Leu Gln Ala Ala Ala Glu Gln Ala Tyr Gly Pro Gly Asn Tyr Ser
325 330 335Pro Asn Leu Phe Val Thr Tyr Trp Ser Phe Arg Ala Met Ile Gly Leu
340 345 350Met Leu Gly Ser Leu Ala Ile Ala Ala Ile Ala Trp Leu Leu Leu Arg
355 360 365Lys Lys Arg Thr Pro Thr Gly Lys Ile Ala Arg Leu Phe Gln Ile Gly
370 375 380Ser Leu Ile Ala Ile Pro Phe Pro Phe Leu Ala Asn Ser Ala Gly Trp385 390 395 400Ile Phe Thr Glu Met Gly Arg Gln Pro Trp Val Val His Pro Asn Pro
405 410 415Glu Ser Ala Gly Asp Ala Arg Thr Glu Met Ile Arg Met Thr Val Asp
420 425 430Met Gly Val Ser Asp His Ala Pro Trp Gln Val Trp Leu Thr Leu Ile
435 440 445Gly Phe Thr Ile Leu Tyr Leu Ile Leu Phe Val Val Trp Val Trp Leu
450 455 460Ile Arg Arg Ala Val Leu Ile Gly Pro Pro Glu Glu Gly Ala Pro Ser465 470 475 480Val Glu Ala Lys Thr Gly Pro Ala Thr Pro Ile Gly Ser Asp Met Pro
485 490 495Met Thr Pro Leu Gln Phe Thr Ala Ala Ala Pro Thr Thr Gly Glu Lys
500 505 510Glu<210>643<211>238<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(238)<223>FRXA00055<400>643tcttattggt tcttcgtttt gtatcgataa atacaatcgg tttcctggct taataaggct 60gttcctgtca acctgcaatg gaagaggaag tgtacctagc gtg gat gtc gtc gac 115
Val Asp Val Val Asp
1 5atc gca cgg tgg caa ttc gga att acc acc gtc tat cac ttc att ttt 163Ile Ala Arg Trp Gln Phe Gly Ile Thr Thr Val Tyr His Phe Ile Phe
10 15 20gtc cca ctg acc att ggc tta gca ccg ctg gtc gcg atc atg caa acg 211Val Pro Leu Thr Ile Gly Leu Ala Pro Leu Val Ala Ile Met Gln Thr
25 30 35ttt tgg caa gtt acc ggc aaa gag cac 238Phe Trp Gln Val Thr Gly Lys Glu His
40 45<210>644<211>46<212>PRT<213>谷氨酸棒杆菌<400>644Val Asp Val Val Asp Ile Ala Arg Trp Gln Phe Gly Ile Thr Thr Val1 5 10 15Tyr His Phe Ile Phe Val Pro Leu Thr Ile Gly Leu Ala Pro Leu Val
20 25 30Ala Ile Met Gln Thr Phe Trp Gln Val Thr Gly Lys Glu His
35 40 45<210>645<211>1325<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1302)<223>FRXA01744<400>645tgg tcg gaa tat tcg cgt ttc gtc ggt gat gtt ttc ggc gga ccg ctg 48Trp Ser Glu Tyr Ser Arg Phe Val Gly Asp Val Phe Gly Gly Pro Leu1 5 10 15gct ttg gag ggt ctt atc gcg ttc ttc ctt gag tct gta ttc ctg gga 96Ala Leu Glu Gly Leu Ile Ala Phe Phe Leu Glu Ser Val Phe Leu Gly
20 25 30ctg tgg att ttc gga tgg ggg aag att cct ggt tgg ttg cac act gca 144Leu Trp Ile Phe Gly Trp Gly Lys Ile Pro Gly Trp Leu His Thr Ala
35 40 45tcc att tgg atc gtt gct att gcg acg aat att tct gcc tat ttc atc 192Ser Ile Trp Ile Val Ala Ile Ala Thr Asn Ile Ser Ala Tyr Phe Ile
50 55 60atc gtg gcc aac tcg ttt atg cag cat ccg gtg ggt gct gag tat aac 240Ile Val Ala Asn Ser Phe Met Gln His Pro Val Gly Ala Glu Tyr Asn65 70 75 80cct gag act ggt cgt gcg gag ctt act gat ttt tgg gct ctt ctc aca 288Pro Glu Thr Gly Arg Ala Glu Leu Thr Asp Phe Trp Ala Leu Leu Thr
85 90 95aac tcc acc gcg ctg gct gcg ttc ccg cat gct gtt gcc ggt ggt ttt 336Asn Ser Thr Ala Leu Ala Ala Phe Pro His Ala Val Ala Gly Gly Phe
100 105 110tta aca gct gga act ttc gtt ctc gga att tcg ggt tgg tgg att att 384Leu Thr Ala Gly Thr Phe Val Leu Gly Ile Ser Gly Trp Trp Ile Ile
115 120 125cgt gcg cac cgt cag gcc aag aag gct gag tcg gaa atc gag tcg aag 432Arg Ala His Arg Gln Ala Lys Lys Ala Glu Ser Glu Ile Glu Ser Lys
130 135 140cat tcg atg cac agg ccc gcg ttg tgg gtt ggt tgg tgg acc aca gtt 480His Ser Met His Arg Pro Ala Leu Trp Val Gly Trp Trp Thr Thr Val145 150 155 160gtc tct tcc gtg gcg ctg ttc atc act ggc gat atc cag gcg aag ctc 528Val Ser Ser Val Ala Leu Phe Ile Thr Gly Asp Ile Gln Ala Lys Leu
165 170 175atg ttc gtg caa cag cca atg aag atg gcg tcg gcg gaa tcc ttg tgt 576Met Phe Val Gln Gln Pro Met Lys Met Ala Ser Ala Glu Ser Leu Cys
180 185 190gaa acc gcc aca gat cca aac ttc tcc att ctg aca att ggt acg cac 624Glu Thr Ala Thr Asp Pro Asn Phe Ser Ile Leu Thr Ile Gly Thr His
195 200 205aac aac tgc gat acg gta acc cac ctg atc gat gtt ccg ttt gtg ctt 672Asn Asn Cys Asp Thr Val Thr His Leu Ile Asp Val Pro Phe Val Leu
210 215 220cca ttc ttg gct gaa gga aaa ttc acc ggt gtg act ttg cag ggt gta 720Pro Phe Leu Ala Glu Gly Lys Phe Thr Gly Val Thr Leu Gln Gly Val225 230 235 240aac cag ctc caa gct gca gcg gag caa gca tac ggt cct ggc aac tac 768Asn Gln Leu Gln Ala Ala Ala Glu Gln Ala Tyr Gly Pro Gly Asn Tyr
245 250 255tcc cct aac ttg ttt gtc acc tac tgg tca ttc cgc gca atg atc ggc 816Ser Pro Asn Leu Phe Val Thr Tyr Trp Ser Phe Arg Ala Met Ile Gly
260 265 270ctg atg ctt ggt tct ttg gct atc gct gcg att gcg tgg ctg ttg ctg 864Leu Met Leu Gly Ser Leu Ala Ile Ala Ala Ile Ala Trp Leu Leu Leu
275 280 285cgt aag aag cgc aca cca act gga aag att gct cgt ctg ttc cag atc 912Arg Lys Lys Arg Thr Pro Thr Gly Lys Ile Ala Arg Leu Phe Gln Ile
290 295 300ggc agc ctc att gct atc ccg ttc cca ttc ttg gcc aac tct gct ggt 960Gly Ser Leu Ile Ala Ile Pro Phe Pro Phe Leu Ala Asn Ser Ala Gly305 310 315 320tgg atc ttc acc gag atg ggc cgc cag cct tgg gtg gtg cac ccg aac 1008Trp Ile Phe Thr Glu Met Gly Arg Gln Pro Trp Val Val His Pro Asn
325 330 335cct gaa tct gcc ggc gat gcc cga aca gag atg atc cgg atg act gtt 1056Pro Glu Ser Ala Gly Asp Ala Arg Thr Glu Met Ile Arg Met Thr Val
340 345 350gat atg ggt gta tct gat cat gcg cca tgg caa gtc tgg ctg act ctc 1104Asp Met Gly Val Ser Asp His Ala Pro Trp Gln Val Trp Leu Thr Leu
355 360 365att ggc ttc acg att ctc tat ctc att ttg ttc gtg gtg tgg gtg tgg 1152Ile Gly Phe Thr Ile Leu Tyr Leu Ile Leu Phe Val Val Trp Val Trp
370 375 380ctg att cgc cgc gca gtt ctg atc gga cca cca gag gaa ggc gct cca 1200Leu Ile Arg Arg Ala Val Leu Ile Gly Pro Pro Glu Glu Gly Ala Pro385 390 395 400tcc gtg gag gca aag act gga ccg gca acc ccg att ggt tca gat atg 1248Ser Val Glu Ala Lys Thr Gly Pro Ala Thr Pro Ile Gly Ser Asp Met
405 410 415ccc atg aca ccg ctg caa ttt act gcc gct gcc cca acc aca ggt gaa 1296Pro Met Thr Pro Leu Gln Phe Thr Ala Ala Ala Pro Thr Thr Gly Glu
420 425 430aag gaa taaccatgga tctcaatacc ttt 1325Lys Glu<210>646<211>434<212>PRT<213>谷氨酸棒杆菌<400>646Trp Ser Glu Tyr Ser Arg Phe Val Gly Asp Val Phe Gly Gly Pro Leu1 5 10 15Ala Leu Glu Gly Leu Ile Ala Phe Phe Leu Glu Ser Val Phe Leu Gly
20 25 30Leu Trp Ile Phe Gly Trp Gly Lys Ile Pro Gly Trp Leu His Thr Ala
35 40 45Ser Ile Trp Ile Val Ala Ile Ala Thr Asn Ile Ser Ala Tyr Phe Ile
50 55 60Ile Val Ala Asn Ser Phe Met Gln His Pro Val Gly Ala Glu Tyr Asn65 70 75 80Pro Glu Thr Gly Arg Ala Glu Leu Thr Asp Phe Trp Ala Leu Leu Thr
85 90 95Asn Ser Thr Ala Leu Ala Ala Phe Pro His Ala Val Ala Gly Gly Phe
100 105 110Leu Thr Ala Gly Thr Phe Val Leu Gly Ile Ser Gly Trp Trp Ile Ile
115 120 125Arg Ala His Arg Gln Ala Lys Lys Ala Glu Ser Glu Ile Glu Ser Lys
130 135 140His Ser Met His Arg Pro Ala Leu Trp Val Gly Trp Trp Thr Thr Val145 150 155 160Val Ser Ser Val Ala Leu Phe Ile Thr Gly Asp Ile Gln Ala Lys Leu
165 170 175Met Phe Val Gln Gln Pro Met Lys Met Ala Ser Ala Glu Ser Leu Cys
180 185 190Glu Thr Ala Thr Asp Pro Asn Phe Ser Ile Leu Thr Ile Gly Thr His
195 200 205Asn Asn Cys Asp Thr Val Thr His Leu Ile Asp Val Pro Phe Val Leu
210 215 220Pro Phe Leu Ala Glu Gly Lys Phe Thr Gly Val Thr Leu Gln Gly Val225 230 235 240Asn Gln Leu Gln Ala Ala Ala Glu Gln Ala Tyr Gly Pro Gly Asn Tyr
245 250 255Ser Pro Asn Leu Phe Val Thr Tyr Trp Ser Phe Arg Ala Met Ile Gly
260 265 270Leu Met Leu Gly Ser Leu Ala Ile Ala Ala Ile Ala Trp Leu Leu Leu
275 280 285Arg Lys Lys Arg Thr Pro Thr Gly Lys Ile Ala Arg Leu Phe Gln Ile
290 295 300Gly Ser Leu Ile Ala Ile Pro Phe Pro Phe Leu Ala Asn Ser Ala Gly305 310 315 320Trp Ile Phe Thr Glu Met Gly Arg Gln Pro Trp Val Val His Pro Asn
325 330 335Pro Glu Ser Ala Gly Asp Ala Arg Thr Glu Met Ile Arg Met Thr Val
340 345 350Asp Met Gly Val Ser Asp His Ala Pro Trp Gln Val Trp Leu Thr Leu
355 360 365Ile Gly Phe Thr Ile Leu Tyr Leu Ile Leu Phe Val Val Trp Val Trp
370 375 380Leu Ile Arg Arg Ala Val Leu Ile Gly Pro Pro Glu Glu Gly Ala Pro385 390 395 400Ser Val Glu Ala Lys Thr Gly Pro Ala Thr Pro Ile Gly Ser Asp Met
405 410 415Pro Met Thr Pro Leu Gln Phe Thr Ala Ala Ala Pro Thr Thr Gly Glu
420 425 430Lys Glu<210>647<211>307<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(307)<223>RXA00379<400>647cgtgctggat aaacagcacc gccccgcagc agtgttcttg cgcgaagtca cctccaaaga 60tgtgttggat gttgcgttgc cattggtaga tgaggcctaa atg tct gag att gtg 115
Met Ser Glu Ile Val
1 5gta gcc caa agc atc ggc cag cag ttt gct gac gtc gca gct tcc ggg 163Val Ala Gln Ser Ile Gly Gln Gln Phe Ala Asp Val Ala Ala Ser Gly
10 15 20cca ctg ttc ctt ggc atc ctt gcc gca gcg ctc gca ggt ctg gtg tct 211Pro Leu Phe Leu Gly Ile Leu Ala Ala Ala Leu Ala Gly Leu Val Ser
25 30 35ttt gcc agc ccg tgt gtt gtg ccg ttg gtg cca gga tat att tcc tac 259Phe Ala Ser Pro Cys Val Val Pro Leu Val Pro Gly Tyr Ile Ser Tyr
40 45 50ctc gcc ggc gtg gtc ggt ggg gaa gtg gaa tac agc gct cat gcc acc 307Leu Ala Gly Val Val Gly Gly Glu Val Glu Tyr Ser Ala His Ala Thr
55 60 65<210>648<211>69<212>PRT<213>谷氨酸棒杆菌<400>648Met Ser Glu Ile Val Val Ala Gln Ser Ile Gly Gln Gln Phe Ala Asp1 5 10 15Val Ala Ala Ser Gly Pro Leu Phe Leu Gly Ile Leu Ala Ala Ala Leu
20 25 30Ala Gly Leu Val Ser Phe Ala Ser Pro Cys Val Val Pro Leu Val Pro
35 40 45Gly Tyr Ile Ser Tyr Leu Ala Gly Val Val Gly Gly Glu Val Glu Tyr
50 55 60Ser Ala His Ala Thr65<210>649<211>362<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(339)<223>RXA00385<400>649ctt ggc gga gtg ttc gct ctc ggt tgg acc cct tgt ttg ggc ccc acc 48Leu Gly Gly Val Phe Ala Leu Gly Trp Thr Pro Cys Leu Gly Pro Thr1 5 10 15ctg gct gcg atc atc tcc att tct gca ggt act gaa ggc atg acc gct 96Leu Ala Ala Ile Ile Ser Ile Ser Ala Gly Thr Glu Gly Met Thr Ala
20 25 30gcg cgt ggc gtg atc tta att gtg ggt tac tgc ctc gga ctg ggg ctg 144Ala Arg Gly Val Ile Leu Ile Val Gly Tyr Cys Leu Gly Leu Gly Leu
35 40 45ccg ttc ctg ctg atc gcg ttg ggc tcc agc aag gca ctc acc gga gtc 192Pro Phe Leu Leu Ile Ala Leu Gly Ser Ser Lys Ala Leu Thr Gly Val
50 55 60gag tgg ttg cgc aag cat tcc cgc acc ctg caa att atc ggc ggt gtg 240Glu Trp Leu Arg Lys His Ser Arg Thr Leu Gln Ile Ile Gly Gly Val65 70 75 80ttt ttg atc ttg gtc gga gta gcg ttg ctc tct ggc tca tgg gca att 288Phe Leu Ile Leu Val Gly Val Ala Leu Leu Ser Gly Ser Trp Ala Ile
85 90 95ttt atc aac tgg gtc cgt cag tgg acc gtt gaa tac ggc gca aca ctg 336Phe Ile Asn Trp Val Arg Gln Trp Thr Val Glu Tyr Gly Ala Thr Leu
100 105 110ctc tagaaaaaga cttttagtag gaa 362Leu<210>650<211>113<212>PRT<213>谷氨酸棒杆菌<400>650Leu Gly Gly Val Phe Ala Leu Gly Trp Thr Pro Cys Leu Gly Pro Thr1 5 10 15Leu Ala Ala Ile Ile Ser Ile Ser Ala Gly Thr Glu Gly Met Thr Ala
20 25 30Ala Arg Gly Val Ile Leu Ile Val Gly Tyr Cys Leu Gly Leu Gly Leu
35 40 45Pro Phe Leu Leu Ile Ala Leu Gly Ser Ser Lys Ala Leu Thr Gly Val
50 55 60Glu Trp Leu Arg Lys His Ser Arg Thr Leu Gln Ile Ile Gly Gly Val65 70 75 80Phe Leu Ile Leu Val Gly Val Ala Leu Leu Ser Gly Ser Trp Ala Ile
85 90 95Phe Ile Asn Trp Val Arg Gln Trp Thr Val Glu Tyr Gly Ala Thr Leu
100 105 110Leu<210>651<211>901<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(901)<223>RXA01743<400>651aggcaaagac tggaccggca accccgattg gttcagatat gcccatgaca ccgctgcaat 60ttactgccgc tgccccaacc acaggtgaaa aggaataacc atg gat ctc aat acc 115
Met Asp Leu Asn Thr
1 5ttt tgg ttt att ctc atc gca ttt ttg ttt gcg gga tac ttt ctc ctc 163Phe Trp Phe Ile Leu Ile Ala Phe Leu Phe Ala Gly Tyr Phe Leu Leu
10 15 20gaa gga ttc gac ttc ggc gtc gga att ttg gca ccc atc atc ggt aaa 211Glu Gly Phe Asp Phe Gly Val Gly Ile Leu Ala Pro Ile Ile Gly Lys
25 30 35gat tca gcg gct agg aac aca gtg atc cgt acg att ggc cct gtc tgg 259Asp Ser Ala Ala Arg Asn Thr Val Ile Arg Thr Ile Gly Pro Val Trp
40 45 50gac gga aat gaa gtg tgg ctg atc gtg gca ggt ggc gct ttg ttt gct 307Asp Gly Asn Glu Val Trp Leu Ile Val Ala Gly Gly Ala Leu Phe Ala
55 60 65gcc ttc cct gag tgg tac gca acg atg ttc tcc gga atg tat ctg ccg 355Ala Phe Pro Glu Trp Tyr Ala Thr Met Phe Ser Gly Met Tyr Leu Pro70 75 80 85ctg ttc ctc gtg ctt gtg tcg ttg atc atg cgc gtg gtg ggc ctt gaa 403Leu Phe Leu Val Leu Val Ser Leu Ile Met Arg Val Val Gly Leu Glu
90 95 100tgg cgc aag aaa gtc gat gat cct cgt tgg caa aag tgg tct gac cgg 451Trp Arg Lys Lys Val Asp Asp Pro Arg Trp Gln Lys Trp Ser Asp Arg
105 110 115gcc atc ttt att ggt tct tgg act cca ccg ctg atg tgg gga ttc atc 499Ala Ile Phe Ile Gly Ser Trp Thr Pro Pro Leu Met Trp Gly Phe Ile
120 125 130ttc gcc aat att ttg cgt ggc atg ccc ctc aag gcg gat cac acc atc 547Phe Ala Asn Ile Leu Arg Gly Met Pro Leu Lys Ala Asp His Thr Ile
135 140 145gat gct gcg gca gcc ctt cct ggc atg gtc aac gtc ttc gcc att ctg 595Ala Ala Ala Ala Ala Leu Pro Gly Met Val Asn Val Phe Ala Ile Leu150 155 160 165ggt gca ctt gcg ttc acc gca ctg ttc gcc ctt cat ggt ctc gca ttc 643Gly Ala Leu Ala Phe Thr Ala Leu Phe Ala Leu His Gly Leu Ala Phe
170 175 180atc cgc ctg aaa act gct ggt cgg gtg cgc acc gat gcg gcg aag gca 691Ile Arg Leu Lys Thr Ala Gly Arg Val Arg Thr Asp Ala Ala Lys Ala
185 190 195gct cca gta gtc gca ctt ctt gct gcg gtg act ggt gga cct ttc gtg 739Ala Pro Val Val Ala Leu Leu Ala Ala Val Thr Gly Gly Pro Phe Val
200 205 210ttg tgg gct gcc atc gca tac ggc cgt tcc tgg tcc tgg atc ctc gca 787Leu Trp Ala Ala Ile Ala Tyr Gly Arg Ser Trp Ser Trp Ile Leu Ala
215 220 225gtg ctg atc atc gca gcg gtt ctc ggt gga gct ttc gca ctg atc aaa 835Val Leu Ile Ile Ala Ala Val Leu Gly Gly Ala Phe Ala Leu Ile Lys230 235 240 245gac cgc gat gga tta agc ttc ctg tcc act tcc gtc gct gtc atc ggt 883Asp Arg Asp Gly Leu Ser Phe Leu Ser Thr Ser Val Ala Val Ile Gly
250 255 260gtc gtt gca ctg ctg ttt 901Val Val Ala Leu Leu Phe
265<210>652<211>267<212>PRT<213>谷氨酸棒杆菌<400>652Met Asp Leu Asn Thr Phe Trp Phe Ile Leu Ile Ala Phe Leu Phe Ala1 5 10 15Gly Tyr Phe Leu Leu Glu Gly Phe Asp Phe Gly Val Gly Ile Leu Ala
20 25 30Pro Ile Ile Gly Lys Asp Ser Ala Ala Arg Asn Thr Val Ile Arg Thr
35 40 45Ile Gly Pro Val Trp Asp Gly Asn Glu Val Trp Leu Ile Val Ala Gly
50 55 60Gly Ala Leu Phe Ala Ala Phe Pro Glu Trp Tyr Ala Thr Met Phe Ser65 70 75 80Gly Met Tyr Leu Pro Leu Phe Leu Val Leu Val Ser Leu Ile Met Arg
85 90 95Val Val Gly Leu Glu Try Arg Lys Lys Val Asp Asp Pro Arg Trp Gln
100 105 110Lys Trp Ser Asp Arg Ala Ile Phe Ile Gly Ser Trp Thr Pro Pro Leu
115 120 125Met Trp Gly Phe Ile Phe Ala Asn Ile Leu Arg Gly Met Pro Leu Lys
130 135 140Ala Asp His Thr Ile Asp Ala Ala Ala Ala Leu Pro Gly Met Val Asn145 150 155 160Val Phe Ala Ile Leu Gly Ala Leu Ala Phe Thr Ala Leu Phe Ala Leu
165 170 175His Gly Leu Ala Phe Ile Arg Leu Lys Thr Ala Gly Arg Val Arg Thr
180 185 190Asp Ala Ala Lys Ala Ala Pro Val Val Ala Leu Leu Ala Ala Val Thr
195 200 205Gly Gly Pro Phe Val Leu Trp Ala Ala Ile Ala Tyr Gly Arg Ser Trp
210 215 220Ser Trp Ile Leu Ala Val Leu Ile Ile Ala Ala Val Leu Gly Gly Ala225 230 235 240Phe Ala Leu Ile Lys Asp Arg Asp Gly Leu Ser Phe Leu Ser Thr Ser
245 250 255Val Ala Val Ile Gly Val Val Ala Leu Leu Phe
260 265<210>653<211>1779<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1756)<223>RXN02480<400>653acctatgacc gctgtggcgc ctagggtcga cgggcacgtc gcccctcaga ggcccgagcc 60gacaggccat gcacgcaagg gcagcaaagc atggttaatg atg acc acc acc gac 115
Met Thr Thr Thr Asp
1 5cac aag cag ctg ggc att atg tac atc att atg tcc ttc agc ttc ttc 163His Lys Gln Leu Gly Ile Met Tyr Ile Ile Met Ser Phe Ser Phe Phe
10 15 20ttc ctc ggt ggc ttg atg gcc ctg ctt atc cga gcg gag ctt ttc acc 211Phe Leu Gly Gly Leu Met Ala Leu Leu Ile Arg Ala Glu Leu Phe Thr
25 30 35cct ggt ctg cag ttc ctg tct aat gag cag ttc aac cag ctg ttc acc 259Pro Gly Leu Gln Phe Leu Ser Asn Glu Gln Phe Asn Gln Leu Phe Thr
40 45 50atg cac gga act gtc atg ctg ctg ctg tac gga act cca att gtt tgg 307Met His Gly Thr Val Met Leu Leu Leu Tyr Gly Thr Pro Ile Val Trp
55 60 65ggt ttt gct aac tac gtc ctg cca ctt cag atc ggt gcg cct gac gta 355Gly Phe Ala Asn Tyr Val Leu Pro Leu Gln Ile Gly Ala Pro Asp Val70 75 80 85gct ttc cca cgt ttg aat gct ttc ggc ttc tgg atc acc acc gtc ggt 403Ala Phe Pro Arg Leu Asn Ala Phe Gly Phe Trp Ile Thr Thr Val Gly
90 95 100ggt gtc gcg atg ctg acc ggc ttc ctg acc ccg ggt ggt gct gcc gac 451Gly Val Ala Met Leu Thr Gly Phe Leu Thr Pro Gly Gly Ala Ala Asp
105 110 115ttc ggt tgg acc atg tac tcc cca ctg tct gac gca att cac tcc cca 499Phe Gly Trp Thr Met Tyr Ser Pro Leu Ser Asp Ala Ile His Ser Pro
120 125 130ggc ctt ggc tct gac atg tgg att gtc ggt gtc ggt gca act ggt att 547Gly Leu Gly Ser Asp Met Trp Ile Val Gly Val Gly Ala Thr Gly Ile
135 140 145ggc tcc gtt gct tcc gca att aac atg ctc acc acc atc ctc tgc ctc 595Gly Ser Val Ala Ser Ala Ile Asn Met Leu Thr Thr Ile Leu Cys Leu150 155 160 165cgc gca cct ggt atg acc atg ttc cgt atg cct att ttc acc tgg aat 643Arg Ala Pro Gly Met Thr Met Phe Arg Met Pro Ile Phe Thr Trp Asn
170 175 180atc ttc gtt gtt tcc gtt ctt gct ctg ctg atc ttc cca ctg ctg ctc 691Ile Phe Val Val Ser Val Leu Ala Leu Leu Ile Phe Pro Leu Leu Leu
185 190 195gct gct gca ctg ggt gtt ctg tat gac cgc aag ctt ggt gga cac ctg 739Ala Ala Ala Leu Gly Val Leu Tyr Asp Arg Lys Leu Gly Gly His Leu
200 205 210tac gat cca gct aac ggc ggc tcc ctc ctg tgg cag cac ctg ttc tgg 787Tyr Asp Pro Ala Asn Gly Gly Ser Leu Leu Trp Gln His Leu Phe Trp
215 220 225ttc ttc gga cac cct gag gtt tac gtt ctg gcg ctg ccg ttc ttc ggc 835Phe Phe Gly His Pro Glu Val Tyr Val Leu Ala Leu Pro Phe Phe Gly230 235 240 245att gtt tct gag atc att cct gtg ttc tcc cgt aag cca atg ttc ggt 883Ile Val Ser Glu Ile Ile Pro Val Phe Ser Arg Lys Pro Met Phe Gly
250 255 260tac gtc ggc ctg atc ttc gca acc ttg tcc att ggt gca ctg tcc atg 931Tyr Val Gly Leu Ile Phe Ala Thr Leu Ser Ile Gly Ala Leu Ser Met
265 270 275gct gtg tgg gct cac cac atg ttc gtt act ggc gca gtt ttg ctt ccg 979Ala Val Trp Ala His His Met Phe Val Thr Gly Ala Val Leu Leu Pro
280 285 290ttc ttc tcc ttc atg acg ttc ctg att tcg gtt cct acc ggc gtt aag 1027Phe Phe Ser Phe Met Thr Phe Leu Ile Ser Val Pro Thr Gly Val Lys
295 300 305ttc ttc aac tgg gtt gga acc atg tgg aag ggt cac atc act tgg gaa 1075Phe Phe Asn Trp Val Gly Thr Met Trp Lys Gly His Ile Thr Trp Glu310 315 320 325acc cca atg atc tgg tct gtt ggc ttc atg gct acc ttc ctc ttc ggt 1123Thr Pro Met Ile Trp Ser Val Gly Phe Met Ala Thr Phe Leu Phe Gly
330 335 340ggt ctg acc ggc att atg ctg gcg tcc cca cca ctg gac ttc cac ttg 1171Gly Leu Thr Gly Ile Met Leu Ala Ser Pro Pro Leu Asp Phe His Leu
345 350 355gct gac tcc tac ttc ctg atc gcg cac ttc cac tac acc ctc ttc ggt 1219Ala Asp Ser Tyr Phe Leu Ile Ala His Phe His Tyr Thr Leu Phe Gly
360 365 370acc gtg gtg ttc gca tcg tgt gca ggc gtt tac ttc tgg ttc ccg aag 1267Thr Val Val Phe Ala Ser Cys Ala Gly Val Tyr Phe Trp Phe Pro Lys
375 380 385atg act ggc cgc atg atg gac gag cgt ctt ggc aag atc cac ttc tgg 1315Met Thr Gly Arg Met Met Asp Glu Arg Leu Gly Lys Ile His Phe Trp390 395 400 405ttg acc ttc gtc ggt ttc cac gga acc ttc ctc atc cag cac tgg gtg 1363Leu Thr Phe Val Gly Phe His Gly Thr Phe Leu Ile Gln His Trp Val
410 415 420ggc aac atg ggt atg cca cgt cgt tac gct gac tac ctg gat tct gat 1411Gly Asn Met Gly Met Pro Arg Arg Tyr Ala Asp Tyr Leu Asp Ser Asp
425 430 435ggt ttc acc atc tac aac cag atc tcc acc gtg ttc tac ttc ctg ctt 1459Gly Phe Thr Ile Tyr Asn Gln Ile Ser Thr Val Phe Tyr Phe Leu Leu
440 445 450ggc ctg tct gtc att cca ttc atc tgg aac gtc ttc aag tcc tgg cgc 1507Gly Leu Ser Val Ile Pro Phe Ile Trp Asn Val Phe Lys Ser Trp Arg
455 460 465tac ggt gag ctc gtt acc gtt gat gat cct tgg ggt tac ggc aac tcc 1555Tyr Gly Glu Leu Val Thr Val Asp Asp Pro Trp Gly Tyr Gly Asn Ser470 475 480 485ctg gag tgg gca acc tcc tgc cct cct cct cgc cac aac ttc gca tcc 1603Leu Glu Trp Ala Thr Ser Cys Pro Pro Pro Arg His Asn Phe Ala Ser
490 495 500ttg cct cgt atc cgc tcc gag cgc cct gcg ttc gag ctg cac tac ccg 1651Leu Pro Arg Ile Arg Ser Glu Arg Pro Ala Phe Glu Leu His Tyr Pro
505 510 515cac atg att gaa cgc atg cgc gca gag gca cac act gga cat cac gat 1699His Met Ile Glu Arg Met Arg Ala Glu Ala His Thr Gly His His Asp
520 525 530gat att aat gct cca gaa ttg ggt acc gcc cca gcc ctt gca tct gac 1747Asp Ile Asn Ala Pro Glu Leu Gly Thr Ala Pro Ala Leu Ala Ser Asp
535 540 545tcc agc cgc taaaagcgtc tgatttaagt cgg 1779Ser Ser Arg550<210>654<211>552<212>PRT<213>谷氨酸棒杆菌<400>654Met Thr Thr Thr Asp His Lys Gln Leu Gly Ile Met Tyr Ile Ile Met1 5 10 15Ser Phe Ser Phe Phe Phe Leu Gly Gly Leu Met Ala Leu Leu Ile Arg
20 25 30Ala Glu Leu Phe Thr Pro Gly Leu Gln Phe Leu Ser Asn Glu Gln Phe
35 40 45Asn Gln Leu Phe Thr Met His Gly Thr Val Met Leu Leu Leu Tyr Gly
50 55 60Thr Pro Ile Val Trp Gly Phe Ala Asn Tyr Val Leu Pro Leu Gln Ile65 70 75 80Gly Ala Pro Asp Val Ala Phe Pro Arg Leu Asn Ala Phe Gly Phe Trp
85 90 95Ile Thr Thr Val Gly Gly Val Ala Met Leu Thr Gly Phe Leu Thr Pro
100 105 110Gly Gly Ala Ala Asp Phe Gly Trp Thr Met Tyr Ser Pro Leu Ser Asp
115 120 125Ala Ile His Ser Pro Gly Leu Gly Ser Asp Met Trp Ile Val Gly Val
130 135 140Gly Ala Thr Gly Ile Gly Ser Val Ala Ser Ala Ile Asn Met Leu Thr145 150 155 160Thr Ile Leu Cys Leu Arg Ala Pro Gly Met Thr Met Phe Arg Met Pro
165 170 175Ile Phe Thr Trp Asn Ile Phe Val Val Ser Val Leu Ala Leu Leu Ile
180 185 190Phe Pro Leu Leu Leu Ala Ala Ala Leu Gly Val Leu Tyr Asp Arg Lys
195 200 205Leu Gly Gly His Leu Tyr Asp Pro Ala Asn Gly Gly Ser Leu Leu Trp
210 215 220Gln His Leu Phe Trp Phe Phe Gly His Pro Glu Val Tyr Val Leu Ala225 230 235 240Leu Pro Phe Phe Gly Ile Val Ser Glu Ile Ile Pro Val Phe Ser Arg
245 250 255Lys Pro Met Phe Gly Tyr Val Gly Leu Ile Phe Ala Thr Leu Ser Ile
260 265 270Gly Ala Leu Ser Met Ala Val Trp Ala His His Met Phe Val Thr Gly
275 280 285Ala Val Leu Leu Pro Phe Phe Ser Phe Met Thr Phe Leu Ile Ser Val
290 295 300Pro Thr Gly Val Lys Phe Phe Asn Trp Val Gly Thr Met Trp Lys Gly305 310 315 320His Ile Thr Trp Glu Thr Pro Met Ile Trp Ser Val Gly Phe Met Ala
325 330 335Thr Phe Leu Phe Gly Gly Leu Thr Gly Ile Met Leu Ala Ser Pro Pro
340 345 350Leu Asp Phe His Leu Ala Asp Ser Tyr Phe Leu Ile Ala His Phe His
355 360 365Tyr Thr Leu Phe Gly Thr Val Val Phe Ala Ser Cys Ala Gly Val Tyr
370 375 380Phe Trp Phe Pro Lys Met Thr Gly Arg Met Met Asp Glu Arg Leu Gly385 390 395 400Lys Ile His Phe Trp Leu Thr Phe Val Gly Phe His Gly Thr Phe Leu
405 410 415Ile Gln His Trp Val Gly Asn Met Gly Met Pro Arg Arg Tyr Ala Asp
420 425 430Tyr Leu Asp Ser Asp Gly Phe Thr Ile Tyr Asn Gln Ile Ser Thr Val
435 440 445Phe Tyr Phe Leu Leu Gly Leu Ser Val Ile Pro Phe Ile Trp Asn Val
450 455 460Phe Lys Ser Trp Arg Tyr Gly Glu Leu Val Thr Val Asp Asp Pro Trp465 470 475 480Gly Tyr Gly Asn Ser Leu Glu Trp Ala Thr Ser Cys Pro Pro Pro Arg
485 490 495His Asn Phe Ala Ser Leu Pro Arg Ile Arg Ser Glu Arg Pro Ala Phe
500 505 510Glu Leu His Tyr Pro His Met Ile Glu Arg Met Arg Ala Glu Ala His
515 520 525Thr Gly His His Asp Asp Ile Asn Ala Pro Glu Leu Gly Thr Ala Pro
530 535 540Ala Leu Ala Ser Asp Ser Ser Arg545 550<210>655<211>385<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(385)<223>FRXA01919<220><221>其它特征<222>345<223>n=a,c,g,or t<220><221>变异体<222>82<223>Xaa=Met,Thr,Lys,or Arg<400>655acctatgacc gctgtggcgc ctagggtcga cgggcacgtc gcccctcaga ggcccgagcc 60gacaggccat gcacgcaagg gcagcaaagc atggttaatg atg acc acc acc gac 115
Met Thr Thr Thr Asp
1 5cac aag cag ctg ggc att atg tac atc att atg tcc ttc agc ttc ttt 163His Lys Gln Leu Gly Ile Met Tyr Ile Ile Met Ser Phe Ser Phe Phe
10 15 20ttc ctc ggt ggc ttg atg gcc ctg ctt atc cga gcg gag ctt ttc acc 211Phe Leu Gly Gly Leu Met Ala Leu Leu Ile Arg Ala Glu Leu Phe Thr
25 30 35cct ggt ctg cag ttc ctg tct aat gag cag ttc aac cag ctg ttc acc 259Pro Gly Leu Gln Phe Leu Ser Asn Glu Gln Phe Asn Gln Leu Phe Thr
40 45 50atg cac gga act gtc atg ctg ctg ctg tac gga act cca att gtt tgg 307Met His Gly Thr Val Met Leu Leu Leu Tyr Gly Thr Pro Ile Val Trp
55 60 65ggt ttt gct aac tac gtc ctg cca ctt cca acc acc ang caa gcc ttc 355Gly Phe Ala Asn Tyr Val Leu Pro Leu Pro Thr Thr Xaa Gln Ala Phe70 75 80 85tct aaa acc cgg gtg aac tct ccc agg gag 385Ser Lys Thr Arg Val Asn Ser Pro Arg Glu
90 95<210>656<211>95<212>PRT<213>谷氨酸棒杆菌<220><221>变异体<222>82<223>Xaa=Met,Thr,Lys,or Arg<400>656Met Thr Thr Thr Asp His Lys Gln Leu Gly Ile Met Tyr Ile Ile Met1 5 10 15Ser Phe Ser Phe Phe Phe Leu Gly Gly Leu Met Ala Leu Leu Ile Arg
20 25 30Ala Glu Leu Phe Thr Pro Gly Leu Gln Phe Leu Ser Asn Glu Gln Phe
35 40 45Asn Gln Leu Phe Thr Met His Gly Thr Val Met Leu Leu Leu Tyr Gly
50 55 60Thr Pro Ile Val Trp Gly Phe Ala Asn Tyr Val Leu Pro Leu Pro Thr65 70 75 80Thr Xaa Gln Ala Phe Ser Lys Thr Arg Val Asn Ser Pro Arg Glu
85 90 95<210>657<211>972<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(949)<223>FRXA02480<400>657tacgttctgg cgctgccgtt cttcggcatt gtttctgaga tcattcctgt gttctcccgt 60aagccaatgt tcgggttacg tcggcctgat cttcgcaacc ttg tcc att ggt gca 115
Leu Ser Ile Gly Ala
1 5ctg tcc atg gct gtg tgg gct cac cac atg ttc gtt act ggc gca gtt 163Leu Ser Met Ala Val Trp Ala His His Met Phe Val Thr Gly Ala Val
10 15 20ttg ctt ccg ttc ttc tcc ttc atg acg ttc ctg att tcg gtt cct acc 211Leu Leu Pro Phe Phe Ser Phe Met Thr Phe Leu Ile Ser Val Pro Thr
25 30 35ggc gtt aag ttc ttc aac tgg gtt gga acc atg tgg aag ggt cac atc 259Gly Val Lys Phe Phe Asn Trp Val Gly Thr Met Trp Lys Gly His Ile
40 45 50act tgg gaa acc cca atg atc tgg tct gtt ggc ttc atg gct acc ttc 307Thr Trp Glu Thr Pro Met Ile Trp Ser Val Gly Phe Met Ala Thr Phe
55 60 65ctc ttc ggt ggt ctg acc ggc att atg ctg gcg tcc cca cca ctg gac 355Leu Phe Gly Gly Leu Thr Gly Ile Met Leu Ala Ser Pro Pro Leu Asp70 75 80 85ttc cac ttg gct gac tcc tac ttc ctg atc gcg cac ttc cac tac acc 403Phe His Leu Ala Asp Ser Tyr Phe Leu Ile Ala His Phe His Tyr Thr
90 95 100ctc ttc ggt acc gtg gtg ttc gca tcg tgt gca ggc gtt tac ttc tgg 451Leu Phe Gly Thr Val Val Phe Ala Ser Cys Ala Gly Val Tyr Phe Trp
105 110 115ttc ccg aag atg act ggc cgc atg atg gac gag cgt ctt ggc aag atc 499Phe Pro Lys Met Thr Gly Arg Met Met Asp Glu Arg Leu Gly Lys Ile
120 125 130cac ttc tgg ttg acc ttc gtc ggt ttc cac gga acc ttc ctc atc cag 547His Phe Trp Leu Thr Phe Val Gly Phe His Gly Thr Phe Leu Ile Gln
135 140 145cac tgg gtg ggc aac atg ggt atg cca cgt cgt tac gct gac tac ctg 595His Trp Val Gly Asn Met Gly Met Pro Arg Arg Tyr Ala Asp Tyr Leu150 155 160 165gat tct gat ggt ttc acc atc tac aac cag atc tcc acc gtg ttc tac 643Asp Ser Asp Gly Phe Thr Ile Tyr Asn Gln Ile Ser Thr Val Phe Tyr
170 175 180ttc ctg ctt ggc ctg tct gtc att cca ttc atc tgg aac gtc ttc aag 691Phe Leu Leu Gly Leu Ser Val Ile Pro Phe Ile Trp Asn Val Phe Lys
185 190 195tcc tgg cgc tac ggt gag ctc gtt acc gtt gat gat cct tgg ggt tac 739Ser Trp Arg Tyr Gly Glu Leu Val Thr Val Asp Asp Pro Trp Gly Tyr
200 205 210ggc aac tcc ctg gag tgg gca acc tcc tgc cct cct cct cgc cac aac 787Gly Asn Ser Leu Glu Trp Ala Thr Ser Cys Pro Pro Pro Arg His Asn
215 220 225ttc gca tcc ttg cct cgt atc cgc tcc gag cgc cct gcg ttc gag ctg 835Phe Ala Ser Leu Pro Arg Ile Arg Ser Glu Arg Pro Ala Phe Glu Leu230 235 240 245cac tac ccg cac atg att gaa cgc atg cgc gca gag gca cac act gga 883His Tyr Pro His Met Ile Glu Arg Met Arg Ala Glu Ala His Thr Gly
250 255 260cat cac gat gat att aat gct cca gaa ttg ggt acc gcc cca gcc ctt 931His His Asp Asp Ile Asn Ala Pro Glu Leu Gly Thr Ala Pro Ala Leu
265 270 275gca tct gac tcc agc cgc taaaagcgtc tgatttaagt cgg 972Ala Ser Asp Ser Ser Arg
280<210>658<211>283<212>PRT<213>谷氨酸棒杆菌<400>658Leu Ser Ile Gly Ala Leu Ser Met Ala Val Trp Ala His His Met Phe1 5 10 15Val Thr Gly Ala Val Leu Leu Pro Phe Phe Ser Phe Met Thr Phe Leu
20 25 30Ile Ser Val Pro Thr Gly Val Lys Phe Phe Asn Trp Val Gly Thr Met
35 40 45Trp Lys Gly His Ile Thr Trp Glu Thr Pro Met Ile Trp Ser Val Gly
50 55 60Phe Met Ala Thr Phe Leu Phe Gly Gly Leu Thr Gly Ile Met Leu Ala65 70 75 80Ser Pro Pro Leu Asp Phe His Leu Ala Asp Ser Tyr Phe Leu Ile Ala
85 90 95His Phe His Tyr Thr Leu Phe Gly Thr Val Val Phe Ala Ser Cys Ala
100 105 110Gly Val Tyr Phe Trp Phe Pro Lys Met Thr Gly Arg Met Met Asp Glu
115 120 125Arg Leu Gly Lys Ile His Phe Trp Leu Thr Phe Val Gly Phe His Gly
130 135 140Thr Phe Leu Ile Gln His Trp Val Gly Asn Met Gly Met Pro Arg Arg145 150 155 160Tyr Ala Asp Tyr Leu Asp Ser Asp Gly Phe Thr Ile Tyr Asn Gln Ile
165 170 175Ser Thr Val Phe Tyr Phe Leu Leu Gly Leu Ser Val Ile Pro Phe Ile
180 185 190Trp Asn Val Phe Lys Ser Trp Arg Tyr Gly Glu Leu Val Thr Val Asp
195 200 205Asp Pro Trp Gly Tyr Gly Asn Ser Leu Glu Trp Ala Thr Ser Cys Pro
210 215 220Pro Pro Arg His Asn Phe Ala Ser Leu Pro Arg Ile Arg Ser Glu Arg225 230 235 240Pro Ala Phe Glu Leu His Tyr Pro His Met Ile Glu Arg Met Arg Ala
245 250 255Glu Ala His Thr Gly His His Asp Asp Ile Asn Ala Pro Glu Leu Gly
260 265 270Thr Ala Pro Ala Leu Ala Ser Asp Ser Ser Arg
275 280<210>659<211>735<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(712)<223>FRXA02481<400>659tgctaactac gtcctgccac ttcagatcgg tgcgcctgac gtagctttcc cacgttgaaa 60tgctttcggc ttctgaatca ccaccgtcgg tggtgtcgcg atg ctg acc ggc ttc 115
Met Leu Thr Gly Phe
1 5cta acc ccg ggt ggt gct gcc gac ttg ggt gga cca tgt act ccc cac 163Leu Thr Pro Gly Gly Ala Ala Asp Leu Gly Gly Pro Cys Thr Pro His
10 15 20tgt ctg acg caa ttc act ccc cag gcc ttg gtt cta aca tgt gga ttg 211Cys Leu Thr Gln Phe Thr Pro Gln Ala Leu Val Leu Thr Cys Gly Leu
25 30 35tcg ggt gtc ggt gca act ggt att ggc tcc gtt gct tcc gca att aac 259Ser Gly Val Gly Ala Thr Gly Ile Gly Ser Val Ala Ser Ala Ile Asn
40 45 50atg ctc acc acc atc ctc tgc ctc cgc gca cct ggt atg acc atg ttc 307Met Leu Thr Thr Ile Leu Cys Leu Arg Ala Pro Gly Met Thr Met Phe
55 60 65cgt atg cct att ttc acc tgg aat atc ttc gtt gtt tcc gtt ctt gct 355Arg Met Pro Ile Phe Thr Trp Asn Ile Phe Val Val Ser Val Leu Ala70 75 80 85ctg ctg atc ttc cca ctg ctg ctc gct gct gca ctg ggt gtt ctg tat 403Leu Leu Ile Phe Pro Leu Leu Leu Ala Ala Ala Leu Gly Val Leu Tyr
90 95 100gac cgc aag ctt ggt gga cac ctg tac gat cca gct aac ggc ggc tcc 451Asp Arg Lys Leu Gly Gly His Leu Tyr Asp Pro Ala Asn Gly Gly Ser
105 110 115ctc ctg tgg cag cac ctg ttc tgg ttc ttc gga cac cct gag gtt tac 499Leu Leu Trp Gln His Leu Phe Trp Phe Phe Gly His Pro Glu Val Tyr
120 125 130gtt ctg gcg ctg ccg ttc ttc ggc att gtt tct gag atc att cct gtg 547Val Leu Ala Leu Pro Phe Phe Gly Ile Val Ser Glu Ile Ile Pro Val
135 140 145ttc tcc cgt aag cca atg ttc ggg tta cgt cgg cct gat ctt cgc aac 595Phe Ser Arg Lys Pro Met Phe Gly Leu Arg Arg Pro Asp Leu Arg Asn150 155 160 165ctt gtc cat tgg tgc act gtc cat ggc tgt gtg ggc tca cca cat gtt 643Leu Val His Trp Cys Thr Val His Gly Cys Val Gly Ser Pro His Val
170 175 180cgt tac tgg cgc agt ttt gct tcc gtt ctt ctc ctt cat gac gtt cct 691Arg Tyr Trp Arg Ser Phe Ala Ser Val Leu Leu Leu His Asp Val Pro
185 190 195gat ttc ggt tcc tac cgg cgt taagttcttc aactgggttg gaa 735Asp Phe Gly Ser Tyr Arg Arg
200<210>660<211>204<212>PRT<213>谷氨酸棒杆菌<400>660Met Leu Thr Gly Phe Leu Thr Pro Gly Gly Ala Ala Asp Leu Gly Gly1 5 10 15Pro Cys Thr Pro His Cys Leu Thr Gln Phe Thr Pro Gln Ala Leu Val
20 25 30Leu Thr Cys Gly Leu Ser Gly Val Gly Ala Thr Gly Ile Gly Ser Val
35 40 45Ala Ser Ala Ile Asn Met Leu Thr Thr Ile Leu Cys Leu Arg Ala Pro
50 55 60Gly Met Thr Met Phe Arg Met Pro Ile Phe Thr Trp Asn Ile Phe Val65 70 75 80Val Ser Val Leu Ala Leu Leu Ile Phe Pro Leu Leu Leu Ala Ala Ala
85 90 95Leu Gly Val Leu Tyr Asp Arg Lys Leu Gly Gly His Leu Tyr Asp Pro
100 105 110Ala Asn Gly Gly Ser Leu Leu Trp Gln His Leu Phe Trp Phe Phe Gly
115 120 125His Pro Glu Val Tyr Val Leu Ala Leu Pro Phe Phe Gly Ile Val Ser
130 135 140Glu Ile Ile Pro Val Phe Ser Arg Lys Pro Met Phe Gly Leu Arg Arg145 150 155 160Pro Asp Leu Arg Asn Leu Val His Trp Cys Thr Val His Gly Cys Val
165 170 175Gly Ser Pro His Val Arg Tyr Trp Arg Ser Phe Ala Ser Val Leu Leu
180 185 190Leu His Asp Val Pro Asp Phe Gly Ser Tyr Arg Arg
195 200<210>661<211>1200<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1177)<223>RXA02140<400>661aacacaagat atggaatcgg ctggcaaata ggctattctg cgaagataga aatgaccgta 60aggtctctgg tttttgtgtg gacaggaagg cagaacacac gtg gaa cag caa aat 115
Val Glu Gln Gln Asn
1 5aag cgt ggt tta aag cgc aag gcc ctg ctt ggc ggt gtc ttg ggc tta 163Lys Arg Gly Leu Lys Arg Lys Ala Leu Leu Gly Gly Val Leu Gly Leu
10 15 20ggt ggc ctc gcc atg gca ggc tgt gaa gtc gcc cct cct ggc ggt gtg 211Gly Gly Leu Ala Met Ala Gly Cys Glu Val Ala Pro Pro Gly Gly Val
25 30 35ctt gga gat ttc cta cgt atg ggt tgg cct gat ggc att acc cct gaa 259Leu Gly Asp Phe Leu Arg Met Gly Trp Pro Asp Gly Ile Thr Pro Glu
40 45 50gca gtg gcc atg ggt aac ttc tgg tca tgg gtc tgg gtt gct gcc tgg 307Ala Val Ala Met Gly Asn Phe Trp Ser Trp Val Trp Val Ala Ala Trp
55 60 65atc atc ggc atc atc atg tgg ggt cta ttc ctc acc gcc atc ttt gcc 355Ile Ile Gly Ile Ile Met Trp Gly Leu Phe Leu Thr Ala Ile Phe Ala70 75 80 85tgg ggc gca aag agg gct gaa aag cgc ggc gag ggt gaa ttc cct aag 403Trp Gly Ala Lys Arg Ala Glu Lys Arg Gly Glu Gly Glu Phe Pro Lys
90 95 100cag ctc cag tac aac gtt cca ctt gag ctc gtt ctg acg atc gtt ccg 451Gln Leu Gln Tyr Asn Val Pro Leu Glu Leu Val Leu Thr Ile Val Pro
105 110 115atc atc att gtt atg gtg ctg ttc ttc ttc acc gtt caa act cag gac 499Ile Ile Ile Val Met Val Leu Phe Phe Phe Thr Val Gln Thr Gln Asp
120 125 130aag gtc acc gct ctg gat aag aac cca gag gtt acc gtg gac gtc acc 547Lys Val Thr Ala Leu Asp Lys Asn Pro Glu Val Thr Val Asp Val Thr
135 140 145gct tac cag tgg aac tgg aag ttc gga tac tcc gaa att gat ggc tca 595Ala Tyr Gln Trp Asn Trp Lys Phe Gly Tyr Ser Glu Ile Asp Gly Ser150 155 160 165ctg gca cct ggt gga cag gat tac caa gga agc gac ccg gag cgt cag 643Leu Ala Pro Gly Gly Gln Asp Tyr Gln Gly Ser Asp Pro Glu Arg Gln
170 175 180gca gct gcc gag gct tcc aag aag gat cct tct gga gat aac cca att 691Ala Ala Ala Glu Ala Ser Lys Lys Asp Pro Ser Gly Asp Asn Pro Ile
185 190 195cac ggc aac tca sag tct gac gtt tct tac ctt gag ttc aac cga att 739His Gly Asn Ser Lys Ser Asp Val Ser Tyr Leu Glu Phe Asn Arg Ile
200 205 210gaa acc ctc gga acc act gat gaa atc cca gtg atg gtt ctt cct gtg 787Glu Thr Leu Gly Thr Thr Asp Glu Ile Pro Val Met Val Leu Pro Val
215 220 225aac acc cca atc gag ttc aac ctc gca tct gct gac gtt gca cac tcc 835Asn Thr Pro Ile Glu Phe Asn Leu Ala Ser Ala Asp Val Ala His Ser230 235 240 245ttc tgg gtt cca gag ttc ctc ttc aag cga gat gct tac gca cac cct 883Phe Trp Val Pro Glu Phe Leu Phe Lys Arg Asp Ala Tyr Ala His Pro
250 255 260gag gca aac aag tcc cag cgt gtc ttc cag att gaa gag atc act gag 931Glu Ala Asn Lys Ser Gln Arg Val Phe Gln Ile Glu Glu Ile Thr Glu
265 270 275gaa ggc gca ttc gtt ggt cgc tgt gca gaa atg tgc ggt act tac cac 979Glu Gly Ala Phe Val Gly Arg Cys Ala Glu Met Cys Gly Thr Tyr His
280 285 290gca atg atg aac ttc gag ctt cgt gtc gtc gat cgc gat tcc ttc gct 1027Ala Met Met Asn Phe Glu Leu Arg Val Val Asp Arg Asp Ser Phe Ala
295 300 305gag tac atc agc ttc cgt gac tcc aac cca gac gca acc aac gct cag 1075Glu Tyr Ile Ser Phe Arg Asp Ser Asn Pro Asp Ala Thr Asn Ala Gln310 315 320 325gca ctt gag cac att ggt caa gct cct tac gct act tcc act agc cca 1123Ala Leu Glu His Ile Gly Gln Ala Pro Tyr Ala Thr Ser Thr Ser Pro
330 335 340ttc gtt tcc gat cgc acc gca acc cgc gac ggc gaa aac act cag agc 1171Phe Val Ser Asp Arg Thr Ala Thr Arg Asp Gly Glu Asn Thr Gln Ser
345 350 355aac gct taagaaggag tggcgaaaaa atg 1200Asn Ala<210>662<211>359<212>PRT<213>谷氨酸棒杆菌<400>662Val Glu Gln Gln Asn Lys Arg Gly Leu Lys Arg Lys Ala Leu Leu Gly1 5 10 15Gly Val Leu Gly Leu Gly Gly Leu Ala Met Ala Gly Cys Glu Val Ala
20 25 30Pro Pro Gly Gly Val Leu Gly Asp Phe Leu Arg Met Gly Trp Pro Asp
35 40 45Gly Ile Thr Pro Glu Ala Val Ala Met Gly Asn Phe Trp Ser Trp Val
50 55 60Trp Val Ala Ala Trp Ile Ile Gly Ile Ile Met Trp Gly Leu Phe Leu65 70 75 80Thr Ala Ile Phe Ala Trp Gly Ala Lys Arg Ala Glu Lys Arg Gly Glu
85 90 95Gly Glu Phe Pro Lys Gln Leu Gln Tyr Asn Val Pro Leu Glu Leu Val
100 105 110Leu Thr Ile Val Pro Ile Ile Ile Val Met Val Leu Phe Phe Phe Thr
115 120 125Val Gln Thr Gln Asp Lys Val Thr Ala Leu Asp Lys Asn Pro Glu Val
130 135 140Thr Val Asp Val Thr Ala Tyr Gln Trp Asn Trp Lys Phe Gly Tyr Ser145 150 155 160Glu Ile Asp Gly Ser Leu Ala Pro Gly Gly Gln Asp Tyr Gln Gly Ser
165 170 175Asp Pro Glu Arg Gln Ala Ala Ala Glu Ala Ser Lys Lys Asp Pro Ser
180 185 190Gly Asp Asn Pro Ile His Gly Asn Ser Lys Ser Asp Val Ser Tyr Leu
195 200 205Glu Phe Asn Arg Ile Glu Thr Leu Gly Thr Thr Asp Glu Ile Pro Val
210 215 220Met Val Leu Pro Val Asn Thr Pro Ile Glu Phe Asn Leu Ala Ser Ala225 230 235 240Asp Val Ala His Ser Phe Trp Val Pro Glu Phe Leu Phe Lys Arg Asp
245 250 255Ala Tyr Ala His Pro Glu Ala Asn Lys Ser Gln Arg Val Phe Gln Ile
260 265 270Glu Glu Ile Thr Glu Glu Gly Ala Phe Val Gly Arg Cys Ala Glu Met
275 280 285Cys Gly Thr Tyr His Ala Met Met Asn Phe Glu Leu Arg Val Val Asp
290 295 300Arg Asp Ser Phe Ala Glu Tyr Ile Ser Phe Arg Asp Ser Asn Pro Asp305 310 315 320Ala Thr Asn Ala Gln Ala Leu Glu His Ile Gly Gln Ala Pro Tyr Ala
325 330 335Thr Ser Thr Ser Pro Phe Val Ser Asp Arg Thr Ala Thr Arg Asp Gly
340 345 350Glu Asn Thr Gln Ser Asn Ala
355<210>663<211>774<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(751)<223>RXA02142<400>663acgaaaagtt cccggaaggt cgattgaaaa gtttgcgaat tgggggaaaa ttcgcatcaa 60aagccgagtt caaactttca attgaaacgg ggggcttgaa gtg act ttg gcc aac 115
Val Thr Leu Ala Asn
1 5caa aca gcc ata cta gat agc gtg acg agc gca gtt gga aat aca ggt 163Gln Thr Ala Ile Leu Asp Ser Val Thr Ser Ala Val Gly Asn Thr Gly
10 15 20atg gca gca cca caa cgt gtt gcg gca ctg aac cgt ccg aat atg gtc 211Met Ala Ala Pro Gln Arg Val Ala Ala Leu Asn Arg Pro Asn Met Val
25 30 35agt gtc ggc acc att gtg ttc ctg tct cag gaa tta atg ttc ttc gcc 259Ser Val Gly Thr Ile Val Phe Leu Ser Gln Glu Leu Met Phe Phe Ala
40 45 50ggg cta ttc gcg atg tac ttc gtg tcc cgt gcg aac gga ctg gca aat 307Gly Leu Phe Ala Met Tyr Phe Val Ser Arg Ala Asn Gly Leu Ala Asn
55 60 65gga tca tgg gga gag cag aca gat cac ctc aac gtg ccc tac gca ctg 355Gly Ser Trp Gly Glu Gln Thr Asp His Leu Asn Val Pro Tyr Ala Leu70 75 80 85ttg att acg gtc att ctg gtg tct tcc tca gtg act tgc cag ttc gga 403Leu Ile Thr Val Ile Leu Val Ser Ser Ser Val Thr Cys Gln Phe Gly
90 95 100gtt ttt gcg gct gaa agg ggt gac gtt tac ggc ctc cgc aag tgg ttc 451Val Phe Ala Ala Glu Arg Gly Asp Val Tyr Gly Leu Arg Lys Trp Phe
105 110 115ttg gtc acg att atc ctc gga tca atc ttc gtg atc gga cag ggc tac 499Leu Val Thr Ile Ile Leu Gly Ser Ile Phe Val Ile Gly Gln Gly Tyr
120 125 130gag tac atc act ctc gta ggt cac gga ctt aca atc cag agc agt gtc 547Glu Tyr Ile Thr Leu Val Gly His Gly Leu Thr Ile Gln Ser Ser Val
135 140 145tac gga tcg gca ttc ttt att aca acc ggt ttc cac gca ctg cac gtg 595Tyr Gly Ser Ala Phe Phe Ile Thr Thr Gly Phe His Ala Leu His Val150 155 160 165atc gcg ggt gtt atg gcc ttc gtt gtg gtt ctt atg aga atc cat aag 643Ile Ala Gly Val Met Ala Phe Val Val Val Leu Met Arg Ile His Lys
170 175 180tcg aag ttc act ccg gca cag gca acc gca gca atg gtt gtg tct tat 691Ser Lys Phe Thr Pro Ala Gln Ala Thr Ala Ala Met Val Val Ser Tyr
185 190 195tac tgg cac ttc gtt gac gtg gtc tgg atc ggc ctc ttc atc act att 739Tyr Trp His Phe Val Asp Val Val Trp Ile Gly Leu Phe Ile Thr Ile
200 205 210tac ttc att cag taggcagtaa ggaatcctca acg 774Tyr Phe Ile Gln
215<210>664<211>217<212>PRT<213>谷氨酸棒杆菌<400>664Val Thr Leu Ala Asn Gln Thr Ala Ile Leu Asp Ser Val Thr Ser Ala1 5 10 15Val Gly Asn Thr Gly Met Ala Ala Pro Gln Arg Val Ala Ala Leu Asn
20 25 30Arg Pro Asn Met Val Ser Val Gly Thr Ile Val Phe Leu Ser Gln Glu
35 40 45Leu Met Phe Phe Ala Gly Leu Phe Ala Met Tyr Phe Val Ser Arg Ala
50 55 60Asn Gly Leu Ala Asn Gly Ser Trp Gly Glu Gln Thr Asp His Leu Asn65 70 75 80Val Pro Tyr Ala Leu Leu Ile Thr Val Ile Leu Val Ser Ser Ser Val
85 90 95Thr Cys Gln Phe Gly Val Phe Ala Ala Glu Arg Gly Asp Val Tyr Gly
100 105 110Leu Arg Lys Trp Phe Leu Val Thr Ile Ile Leu Gly Ser Ile Phe Val
115 120 125Ile Gly Gln Gly Tyr Glu Tyr Ile Thr Leu Val Gly His Gly Leu Thr
130 135 140Ile Gln Ser Ser Val Tyr Gly Ser Ala Phe Phe Ile Thr Thr Gly Phe145 150 155 160His Ala Leu His Val Ile Ala Gly Val Met Ala Phe Val Val Val Leu
165 170 175Met Arg Ile His Lys Ser Lys Phe Thr Pro Ala Gln Ala Thr Ala Ala
180 185 190Met Val Val Ser Tyr Tyr Trp His Phe Val Asp Val Val Trp Ile Gly
195 200 205Leu Phe Ile Thr Ile Tyr Phe Ile Gln
210 215<210>665<211>1347<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1324)<223>RXA02144<400>665actcactcgg tagcttgggc ccagtggctg agggtctgtt catgtgggta ttcggcatct 60tggtcctcgt ggccgccgct atgtggattg gatcacgttc atg agt aac aac aac 115
Met Ser Asn Asn Asn
1 5gac aaa cag tac aca acc caa gaa ctc aac gcg atg agc aat gag gat 163Asp Lys Gln Tyr Thr Thr Gln Glu Leu Asn Ala Met Ser Asn Glu Asp
10 15 20ctt gca cga ctt ggt aca gag ctg gac gac gtt acc att gca tac cgc 211Leu Ala Arg Leu Gly Thr Glu Leu Asp Asp Val Thr Ile Ala Tyr Arg
25 30 35aag gaa cgt ttc cca atc gct aat gac cca gct gag aag cgc gct gca 259Lys Glu Arg Phe Pro Ile Ala Asn Asp Pro Ala Glu Lys Arg Ala Ala
40 45 50cgt gca gtt act ttc tgg cta gtc ctc ggc atc att ggt gga ctt ggg 307Arg Ala Val Thr Phe Trp Leu Val Leu Gly Ile Ile Gly Gly Leu Gly
55 60 65ttc ctg gct acc tac att ttc tgg cct tgg gag tac aag gca cac gga 355Phe Leu Ala Thr Tyr Ile Phe Trp Pro Trp Glu Tyr Lys Ala His Gly70 75 80 85gat gaa ggt ctc ctg gcg tac acc ttg tac acc cca atg ctg ggt att 403Asp Glu Gly Leu Leu Ala Tyr Thr Leu Tyr Thr Pro Met Leu Gly Ile
90 95 100act tcc ggt ctt tgc atc ctg tcc ctg gga ttt gca gtt gtc ctt tat 451Thr Ser Gly Leu Cys Ile Leu Ser Leu Gly Phe Ala Val Val Leu Tyr
105 110 115gtc aag aag ttc att cca gag gaa atc gca gta cag cgt cgc cac gac 499Val Lys Lys Phe Ile Pro Glu Glu Ile Ala Val Gln Arg Arg His Asp
120 125 130ggt cct tct gaa gaa gtt gac cgc cgc acc atc gtt gca ctt ctc aat 547Gly Pro Ser Glu Glu Val Asp Arg Arg Thr Ile Val Ala Leu Leu Asn
135 140 145gac tct tgg cag acc tct act ctt ggt cgt cgc aag ctg atc atg gga 595Asp Ser Trp Gln Thr Ser Thr Leu Gly Arg Arg Lys Leu Ile Met Gly150 155 160 165ctt gca ggt ggc gga gca gta ctg gcc ggc ctg acc atc atc gct cca 643Leu Ala Gly Gly Gly Ala Val Leu Ala Gly Leu Thr Ile Ile Ala Pro
170 175 180atg ggc ggt atg atc aag aac cct tgg aat cct aag gaa ggc cca atg 691Met Gly Gly Met Ile Lys Asn Pro Trp Asn Pro Lys Glu Gly Pro Met
185 190 195gac gtt cag ggt gac ggc acc ctg tgg act tcc ggt tgg act ctc gtt 739Asp Val Gln Gly Asp Gly Thr Leu Trp Thr Ser Gly Trp Thr Leu Val
200 205 210gag aac gac gtc aag gtt tac ctc ggc cgc gac act gca gca att gcg 787Glu Asn Asp Val Lys Val Tyr Leu Gly Arg Asp Thr Ala Ala Ile Ala
215 220 225gag tcc cac acc gat gca acc ggt gag cac tgg tca acc act ggt gtt 835Glu Ser His Thr Asp Ala Thr Gly Glu His Trp 5er Thr Thr Gly Val230 235 240 245tcc cgc ctg gtt cgt atg cgc cca gaa gat ctg gca gca gca tcc atg 883Ser Arg Leu Val Arg Met Arg Pro Glu Asp Leu Ala Ala Ala Ser Met
250 255 260gaa act gtc ttc cca ctt cca gct gaa atg gtg aac gac ggt gct gaa 931Glu Thr Val Phe Pro Leu Pro Ala Glu Met Val Asn Asp Gly Ala Glu
265 270 275tac gat cct gcg aag gac gtc tac gag cac caa atg cac tcg gtg cac 979Tyr Asp Pro Ala Lys Asp Val Tyr Glu His Gln Met His Ser Val His
280 285 290ggc cca cgc aac gca gtt atg ttg atc cgt ctc cgt acc gct gac gct 1027Gly Pro Arg Asn Ala Val Met Leu Ile Arg Leu Arg Thr Ala Asp Ala
295 300 305gaa aag gtt atc gaa cgc gaa ggc cag gag tcc ttc cac tac ggt gac 1075Glu Lys Val Ile Glu Arg Glu Gly Gln Glu Ser Phe His Tyr Gly Asp310 315 320 325tac tac gct tac tcc aag att tgt aca cac att ggt tgc cca acc tca 1123Tyr Tyr Ala Tyr Ser Lys Ile Cys Thr His Ile Gly Cys Pro Thr Ser
330 335 340ctg tac gag gct cag acc aat cgt att ctg tgc cca tgt cac cag tcg 1171Leu Tyr Glu Ala Gln Thr Asn Arg Ile Leu Cys Pro Cys His Gln Ser
345 350 355cag ttt gac gca ttg cac tac gga aag cca gtc ttt gga cct gct gcc 1219Gln Phe Asp Ala Leu His Tyr Gly Lys Pro Val Phe Gly Pro Ala Ala
360 365 370cgt gca ctg cca cag ctg cca att acc gtt gat gaa gag ggc tac ctc 1267Arg Ala Leu Pro Gln Leu Pro Ile Thr Val Asp Glu Glu Gly Tyr Leu
375 380 385atc gcc gct ggt aac ttc att gag cca ctc ggc cct gca ttc tgg gag 1315Ile Ala Ala Gly Asn Phe Ile Glu Pro Leu Gly Pro Ala Phe Trp Glu390 395 400 405cgt aag tca tgagtctagc taccgtggga aac 1347Arg Lys Ser<210>666<211>408<212>PRT<213>谷氨酸棒杆菌<400>666Met Ser Asn Asn Asn Asp Lys Gln Tyr Thr Thr Gln Glu Leu Asn Ala1 5 10 15Met Ser Asn Glu Asp Leu Ala Arg Leu Gly Thr Glu Leu Asp Asp Val
20 25 30Thr Ile Ala Tyr Arg Lys Glu Arg Phe Pro Ile Ala Asn Asp Pro Ala
35 40 45Glu Lys Arg Ala Ala Arg Ala Val Thr Phe Trp Leu Val Leu Gly Ile
50 55 60Ile Gly Gly Leu Gly Phe Leu Ala Thr Tyr Ile Phe Trp Pro Trp Glu65 70 75 80Tyr Lys Ala His Gly Asp Glu Gly Leu Leu Ala Tyr Thr Leu Tyr Thr
85 90 95Pro Met Leu Gly Ile Thr Ser Gly Leu Cys Ile Leu Ser Leu Gly Phe
100 105 110Ala Val Val Leu Tyr Val Lys Lys Phe Ile Pro Glu Glu Ile Ala Val
115 120 125Gln Arg Arg His Asp Gly Pro Ser Glu Glu Val Asp Arg Arg Thr Ile
130 135 140Val Ala Leu Leu Asn Asp Ser Trp Gln Thr Ser Thr Leu Gly Arg Arg145 150 155 160Lys Leu Ile Met Gly Leu Ala Gly Gly Gly Ala Val Leu Ala Gly Leu
165 170 175Thr Ile Ile Ala Pro Met Gly Gly Met Ile Lys Asn Pro Trp Asn Pro
180 185 190Lys Glu Gly Pro Met Asp Val Gln Gly Asp Gly Thr Leu Trp Thr Ser
195 200 205Gly Trp Thr Leu Val Glu Asn Asp Val Lys Val Tyr Leu Gly Arg Asp
210 215 220Thr Ala Ala Ile Ala Glu Ser His Thr Asp Ala Thr Gly Glu His Trp225 230 235 240Ser Thr Thr Gly Val Ser Arg Leu Val Arg Met Arg Pro Glu Asp Leu
245 250 255Ala Ala Ala Ser Met Glu Thr Val Phe Pro Leu Pro Ala Glu Met Val
260 265 270Asn Asp Gly Ala Glu Tyr Asp Pro Ala Lys Asp Val Tyr Glu His Gln
275 280 285Met His Ser Val His Gly Pro Arg Asn Ala Val Met Leu Ile Arg Leu
290 295 300Arg Thr Ala Asp Ala Glu Lys Val Ile Glu Arg Glu Gly Gln Glu Ser305 310 315 320Phe His Tyr Gly Asp Tyr Tyr Ala Tyr Ser Lys Ile Cys Thr His Ile
325 330 335Gly Cys Pro Thr Ser Leu Tyr Glu Ala Gln Thr Asn Arg Ile Leu Cys
340 345 350Pro Cys His Gln Ser Gln Phe Asp Ala Leu His Tyr Gly Lys Pro Val
355 360 365Phe Gly Pro Ala Ala Arg Ala Leu Pro Gln Leu Pro Ile Thr Val Asp
370 375 380Glu Glu Gly Tyr Leu Ile Ala Ala Gly Asn Phe Ile Glu Pro Leu Gly385 390 395 400Pro Ala Phe Trp Glu Arg Lys Ser
405<210>667<211>1053<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1030)<223>RXA02740<400>667aacgcaaggt gcacactcac cgcagggata tttaaataat aaggactcac aacttaaata 60tatgagtgat ttgaaaatgc aacgttctgg aggagaaccc ttg gac acg atc aag 115
Leu Asp Thr Ile Lys
1 5gcc tat att gcg cta acg aag ccc agg gtt att gaa ctc ctc ctt gtc 163Ala Tyr Ile Ala Leu Thr Lys Pro Arg Val Ile Glu Leu Leu Leu Val
10 15 20gcc aca atc ccc aca atg ctt cag gct gaa cgc ggt gag aac aac att 211Ala Thr Ile Pro Thr Met Leu Gln Ala Glu Arg Gly Glu Asn Asn Ile
25 30 35gtg ctc atc ttg ctg act gtg ttc ggt ggc tgg atg ggt gcg gcc gcc 259Val Leu Ile Leu Leu Thr Val Phe Gly Gly Trp Met Gly Ala Ala Ala
40 45 50gcc aac acc ttc aac atg gtg gca gac tcc gat att gat cag cgc atg 307Ala Asn Thr Phe Asn Met Val Ala Asp Ser Asp Ile Asp Gln Arg Met
55 60 65gga cgc act agg gct cgc cct ttg gtg cgc cac acc gtg agt aat cgc 355Gly Arg Thr Arg Ala Arg Pro Leu Val Arg His Thr Val Ser Asn Arg70 75 80 85gac gcc tcc att ttt gcg tgg gtc ctg aca gtg gcc agc ttc ttg tgg 403Asp Ala Ser Ile Phe Ala Trp Val Leu Thr Val Ala Ser Phe Leu Trp
90 95 100ctg tgg ctg ctg tgc gat tcg atg ctc gcc ggc atc ttc gtg ttg atc 451Leu Trp Leu Leu Cys Asp Ser Met Leu Ala Gly Ile Phe Val Leu Ile
105 110 115acg att ttc ttc tac att ttt gtc tac acc aag tgg ctg aag cgc cgc 499Thr Ile Phe Phe Tyr Ile Phe Val Tyr Thr Lys Trp Leu Lys Arg Arg
120 125 130acg cac atg aat atc gtg tgg ggc gga gcc gca ggt tgt atg cca gtg 547Thr His Met Asn Ile Val Trp Gly Gly Ala Ala Gly Cys Met Pro Val
135 140 145ctc gtc ggc tgg gca gtg atc gtt gat cag ttt gag cca ggc gtt cca 595Leu Val Gly Trp Ala Val Ile Val Asp Gln Phe Glu Pro Gly Val Pro150 155 160 165cag cag tgg tgg cag gca att gtc ctg ttc atg gtg att ttc ttc tgg 643Gln Gln Trp Trp Gln Ala Ile Val Leu Phe Met Val Ile Phe Phe Trp
170 175 180acc cca cct cac acc tgg gct ctg gcc atg aag tac cgc gaa gac tac 691Thr Pro Pro His Thr Trp Ala Leu Ala Met Lys Tyr Arg Glu Asp Tyr
185 190 195aag gcg gct ggc gtc cca atg ctt cct gtc gtg cgc acc cca gtc cag 739Lys Ala Ala Gly Val Pro Met Leu Pro Val Val Arg Thr Pro Val Gln
200 205 210gtc acc gca caa atc gtg tgg tac tcc gtg gca act gtg ctg acc acc 787Val Thr Ala Gln Ile Val Trp Tyr Ser Val Ala Thr Val Leu Thr Thr
215 220 225ttc ttg ctc atc cca gca act ggt tgg atc tac gca gcg atc gcc gtc 835Phe Leu Leu Ile Pro Ala Thr Gly Trp Ile Tyr Ala Ala Ile Ala Val230 235 240 245att tcc ggc gtc acc ttc ttg ttc atg gcc atc aag ctg cac ctc ggc 883Ile Ser Gly Val Thr Phe Leu Phe Met Ala Ile Lys Leu His Leu Gly
250 255 260atc aaa aac ggt ggc aag gtc aag cct ctg aag ctg ttt att ttg tcc 931Ile Lys Asn Gly Gly Lys Val Lys Pro Leu Lys Leu Phe Ile Leu Ser
265 270 275aac aac tac ttg gca gtc ctc ttc gtg gca ttg tcc gtc gac gcg gtc 979Asn Asn Tyr Leu Ala Val Leu Phe Val Ala Leu Ser Val Asp Ala Val
280 285 290ctc ggc ctt gag acc atc ggc gag atg ctc ggc tgg acc acc acc ttc 1027Leu Gly Leu Glu Thr Ile Gly Glu Met Leu Gly Trp Thr Thr Thr Phe
295 300 305ttc taaaagcttg cttttcgacg aaa 1053Phe310<210>668<211>310<212>PRT<213>谷氨酸棒杆菌<400> 668Leu Asp Thr Ile Lys Ala Tyr Ile Ala Leu Thr Lys Pro Arg Val Ile1 5 10 15Glu Leu Leu Leu Val Ala Thr Ile Pro Thr Met Leu Gln Ala Glu Arg
20 25 30Gly Glu Asn Asn Ile Val Leu Ile Leu Leu Thr Val Phe Gly Gly Trp
35 40 45Met Gly Ala Ala Ala Ala Asn Thr Phe Asn Met Val Ala Asp Ser Asp
50 55 60Ile Asp Gln Arg Met Gly Arg Thr Arg Ala Arg Pro Leu Val Arg His65 70 75 80Thr Val Ser Asn Arg Asp Ala Ser Ile Phe Ala Trp Val Leu Thr Val
85 90 95Ala Ser Phe Leu Trp Leu Trp Leu Leu Cys Asp Ser Met Leu Ala Gly
100 105 110Ile Phe Val Leu Ile Thr Ile Phe Phe Tyr Ile Phe Val Tyr Thr Lys
115 120 125Trp Leu Lys Arg Arg Thr His Met Asn Ile Val Trp Gly Gly Ala Ala
130 135 140Gly Cys Met Pro Val Leu Val Gly Trp Ala Val Ile Val Asp Gln Phe145 150 155 160Glu Pro Gly Val Pro Gln Gln Trp Trp Gln Ala Ile Val Leu Phe Met
165 170 175Val Ile Phe Phe Trp Thr Pro Pro His Thr Trp Ala Leu Ala Met Lys
180 185 190Tyr Arg Glu Asp Tyr Lys Ala Ala Gly Val Pro Met Leu Pro Val Val
195 200 205Arg Thr Pro Val Gln Val Thr Ala Gln Ile Val Trp Tyr Ser Val Ala
210 215 220Thr Val Leu Thr Thr Phe Leu Leu Ile Pro Ala Thr Gly Trp Ile Tyr225 230 235 240Ala Ala Ile Ala Val Ile Ser Gly Val Thr Phe Leu Phe Met Ala Ile
245 250 255Lys Leu His Leu Gly Ile Lys Asn Gly Gly Lys Val Lys Pro Leu Lys
260 265 270Leu Phe Ile Leu Ser Asn Asn Tyr Leu Ala Val Leu Phe Val Ala Leu
275 280 285Ser Val Asp Ala Val Leu Gly Leu Glu Thr Ile Gly Glu Met Leu Gly
290 295 300Trp Thr Thr Thr Phe Phe305 310<210>669<211>1161<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1138)<223>RXA02743<400>669atatccttaa aagttcccgg gagtttcagt ggcaaatacc accacttcca taccgggaac 60aattgtataa aactagccat gacctgctag gatcagcgac gtg tct act tca gat 115
Val Ser Thr Ser Asp
1 5gct ccc tca aat aat cca gtt gag ttg aag ccc att act ttc tgg gca 163Ala Pro Ser Asn Asn Pro Val Glu Leu Lys Pro Ile Thr Phe Trp Ala
10 15 20ccg acc atc aaa gtg cag cgc att ctc gcg ctc cta ctg ttg att ttc 211Pro Thr Ile Lys Val Gln Arg Ile Leu Ala Leu Leu Leu Leu Ile Phe
25 30 35cag gga ggc atc acc gtt acg ggc tct atc gtc cgt gtc aca ggc tcc 259Gln Gly Gly Ile Thr Val Thr Gly Ser Ile Val Arg Val Thr Gly Ser
40 45 50ggc ctc ggt tgt gat acc tgg cca cta tgc cac gaa ggt tca cta gtc 307Gly Leu Gly Cys Asp Thr Trp Pro Leu Cys His Glu Gly Ser Leu Val
55 60 65cca gtc gca ggc gca gca cca tgg atc cac cag gca gtg gaa ttt ggt 355Pro Val Ala Gly Ala Ala Pro Trp Ile His Gln Ala Val Glu Phe Gly70 75 80 85aac cgc atg ctc act ttc gtg ctt gct gcc gca gcg ctt gcg ttg ttc 403Asn Arg Met Leu Thr Phe Val Leu Ala Ala Ala Ala Leu Ala Leu Phe
90 95 100att gca gtt ctt ggc gca aaa cgc cgc cgc gag atc ctg gtc cat tcc 451Ile Ala Val Leu Gly Ala Lys Arg Arg Arg Glu Ile Leu Val His Ser
105 110 115ttc atc cag ggt ttg ggc atc atc ttg cag gct gtc atc ggt ggc atc 499Phe Ile Gln Gly Leu Gly Ile Ile Leu Gln Ala Val Ile Gly Gly Ile
120 125 130acc gtg ctg gtt gat ttg cac tgg tac gcc gtt gct ttg cac ttc ctg 547Thr Val Leu Val Asp Leu His Trp Tyr Ala Val Ala Leu His Phe Leu
135 140 145cca tcc atg atc ctt gtt ttc atg gcc gcg att ttg tac acc cgc atc 595Pro Ser Met Ile Leu Val Phe Met Ala Ala Ile Leu Tyr Thr Arg Ile150 155 160 165ggc gag ccc gat gac ggc gag att acc acc aca ttc ccc acg tgg atc 643Gly Glu Pro Asp Asp Gly Glu Ile Thr Thr Thr Phe Pro Thr Trp Ile
170 175 180cgc aat gta gct gtc att ggt gca gta gcg ctc tcc gta gta ctg atc 691Arg Asn Val Ala Val Ile Gly Ala Val Ala Leu Ser Val Val Leu Ile
185 190 195acc ggc acc atg acc acc ggc gct ggc gtt cac tct ggc gat gca tca 739Thr Gly Thr Met Thr Thr Gly Ala Gly Val His Ser Gly Asp Ala Ser
200 205 210atc acc atg gat gat cgc ctc gat gtc aac atc gac ttg atg gcc cac 787Ile Thr Met Asp Asp Arg Leu Asp Val Ser Ile Asp Leu Met Ala His
215 220 225atc cac ggc tac agc atg tac atc tac ctc ttc ttc acc ctc atc gtg 835Ile His Gly Tyr Ser Met Tyr Ile Tyr Leu Phe Phe Thr Leu Ile Val230 235 240 245gtc gcc ggt ctg tac aag gca aaa acc acc aag cac aac aag cag ctt 883Val Ala Gly Leu Tyr Lys Ala Lys Thr Thr Lys His Asn Lys Gln Leu
250 255 260ggc ctc atg ctg att ctg ttc att ctg att cag gca ggt atc ggc atc 931Gly Leu Met Leu Ile Leu Phe Ile Leu Ile Gln Ala Gly Ile Gly Ile
265 270 275ttg cag tac cgc atg ggt gtg cca cgc tgg agc atc cca ttc cac atc 979Leu Gln Tyr Arg Met Gly Val Pro Arg Trp Ser Ile Pro Phe His Ile
280 285 290gca atg tct tct gtc gtt gtt gcc ttc act tcc ctt ctg tgg gcg cag 1027Ala Met Ser Ser Val Val Val Ala Phe Thr Ser Leu Leu Trp Ala Gln
295 300 305ggt cgt ata cgc gtc ggc ggt aaa gcc acc gtt act ggt tct gtt gat 1075Gly Arg Ile Arg Val Gly Gly Lys Ala Thr Val Thr Gly Ser Val Asp310 315 320 325ggc gat att aag aac gag atc att acg aac ccc ttt gag aag aaa tca 1123Gly Asp Ile Lys Asn Glu Ile Ile Thr Asn Pro Phe Glu Lys Lys Ser
330 335 340aag cag cct gtt aaa taacacgcaa ctgtatcggt aaa 1161Lys Gln Pro Val Lys
345<210>670<211>346<212>PRT<213>谷氨酸棒杆菌<400>670Val Ser Thr Ser Asp Ala Pro Ser Asn Asn Pro Val Glu Leu Lys Pro1 5 10 15Ile Thr Phe Trp Ala Pro Thr Ile Lys Val Gln Arg Ile Leu Ala Leu
20 25 30Leu Leu Leu Ile Phe Gln Gly Gly Ile Thr Val Thr Gly Ser Ile Val
35 40 45Arg Val Thr Gly Ser Gly Leu Gly Cys Asp Thr Trp Pro Leu Cys His
50 55 60Glu Gly Ser Leu Val Pro Val Ala Gly Ala Ala Pro Trp Ile His Gln65 70 75 80Ala Val Glu Phe Gly Asn Arg Met Leu Thr Phe Val Leu Ala Ala Ala
85 90 95Ala Leu Ala Leu Phe Ile Ala Val Leu Gly Ala Lys Arg Arg Arg Glu
100 105 110Ile Leu Val His Ser Phe Ile Gln Gly Leu Gly Ile Ile Leu Gln Ala
115 120 125Val Ile Gly Gly Ile Thr Val Leu Val Asp Leu His Trp Tyr Ala Val
130 135 140Ala Leu His Phe Leu Pro Ser Met Ile Leu Val Phe Met Ala Ala Ile145 150 155 160Leu Tyr Thr Arg Ile Gly Glu Pro Asp Asp Gly Glu Ile Thr Thr Thr
165 170 175Phe Pro Thr Trp Ile Arg Asn Val Ala Val Ile Gly Ala Val Ala Leu
180 185 190Ser Val Val Leu Ile Thr Gly Thr Met Thr Thr Gly Ala Gly Val His
195 200 205Ser Gly Asp Ala Ser Ile Thr Met Asp Asp Arg Leu Asp Val Ser Ile
210 215 220Asp Leu Met Ala His Ile His Gly Tyr Ser Met Tyr Ile Tyr Leu Phe225 230 235 240Phe Thr Leu Ile Val Val Ala Gly Leu Tyr Lys Ala Lys Thr Thr Lys
245 250 255His Asn Lys Gln Leu Gly Leu Met Leu Ile Leu Phe Ile Leu Ile Gln
260 265 270Ala Gly Ile Gly Ile Leu Gln Tyr Arg Met Gly Val Pro Arg Trp Ser
275 280 285Ile Pro Phe His Ile Ala Met Ser Ser Val Val Val Ala Phe Thr Ser
290 295 300Leu Leu Trp Ala Gln Gly Arg Ile Arg Val Gly Gly Lys Ala Thr Val305 310 315 320Thr Gly Ser Val Asp Gly Asp Ile Lys Asn Glu Ile Ile Thr Asn Pro
325 330 335Phe Glu Lys Lys Ser Lys Gln Pro Val Lys
340 345<210>671<211>444<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(421)<223>RXA01227<400>671ggataatcga aaatatgtgc cccttggtga agggtcgggg agctaatagg atgacagtga 60acctattttc cacgtcttta tccgtagtat tggagatccg atg acc tac aca atc 115
Met Thr Tyr Thr Ile
1 5gcc cag ccc tgc gtt gat gtc ctg gat cga gcc tgc gtc gag gaa tgt 163Ala Gln Pro Cys Val Asp Val Leu Asp Arg Ala Cys Val Glu Glu Cys
10 15 20ccc gtg gac tgc atc tac gag ggc aaa cgg atg ctc tac atc cac ccc 211Pro Val Asp Cys Ile Tyr Glu Gly Lys Arg Met Leu Tyr Ile His Pro
25 30 35gat gag tgc gtc gac tgc ggt gcc tgc gag ccc gtc tgc ccg gtt gaa 259Asp Glu Cys Val Asp Cys Gly Ala Cys Glu Pro Val Cys Pro Val Glu
40 45 50gcc atc ttc tac gaa gat gat gtt ccc cac gaa tgg tgg gac tac acc 307Ala Ile Phe Tyr Glu Asp Asp Val Pro His Glu Trp Trp Asp Tyr Thr
55 60 65ggc gct aac gcc gcc ttt ttc gac gac ctc ggt tcg cca ggc ggt gcc 355Gly Ala Asn Ala Ala Phe Phe Asp Asp Leu Gly Ser Pro Gly Gly Ala70 75 80 85gcc agc ctg ggt ccg cag gac ttc gac gcc cag ctc gtc gcg gtg ctg 403Ala Ser Leu Gly Pro Gln Asp Phe Asp Ala Gln Leu Val Ala Val Leu
90 95 100ccg cca cag aac cag aac taggacctga tatcggccct aaa 444Pro Pro Gln Asn Gln Asn
105<210>672<211>107<212>PRT<213>谷氨酸棒杆菌<400>672Met Thr Tyr Thr Ile Ala Gln Pro Cys Val Asp Val Leu Asp Arg Ala1 5 10 15Cys Val Glu Glu Cys Pro Val Asp Cys Ile Tyr Glu Gly Lys Arg Met
20 25 30Leu Tyr Ile His Pro Asp Glu Cys Val Asp Cys Gly Ala Cys Glu Pro
35 40 45Val Cys Pro Val Glu Ala Ile Phe Tyr Glu Asp Asp Val Pro His Glu
50 55 60Trp Trp Asp Tyr Thr Gly Ala Asn Ala Ala Phe Phe Asp Asp Leu Gly65 70 75 80Ser Pro Gly Gly Ala Ala Ser Leu Gly Pro Gln Asp Phe Asp Ala Gln
85 90 95Leu Val Ala Val Leu Pro Pro Gln Asn Gln Asn
100 105<210>673<211>438<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(415)<223>RXA01865<400>673ggtggaattt ggcctgcggt caaggggaag tagcataata agcctaaagc tttcccatat 60ttattagcct cttagagttc tcaggagaaa acgaaatccc atg aca tac aca atc 115
Met Thr Tyr Thr Ile
1 5gca cag ccc tgc gtt gac gtc ttg gat cgt gcc tgc gtt gaa gaa tgc 163Ala Gln Pro Cys Val Asp Val Leu Asp Arg Ala Cys Val Glu Glu Cys
10 15 20cca gta gat tgc atc tac gaa ggt aag cgc atg ctg tac atc cac ccg 211Pro Val Asp Cys Ile Tyr Glu Gly Lys Arg Met Leu Tyr Ile His Pro
25 30 35gat gag tgc gtt gac tgt ggt gca tgt gag cct gct tgc cca gtt gag 259Asp Glu Cys Val Asp Cys Gly Ala Cys Glu Pro Ala Cys Pro Val Glu
40 45 50gca atc ttc tac gag gac gat gtc cca gac gaa tgg ctt gac tac aac 307Ala Ile Phe Tyr Glu Asp Asp Val Pro Asp Glu Trp Leu Asp Tyr Asn
55 60 65gat gcc aac gct gca ttc ttc gat gat ctg ggc tcc cca ggt ggt gcg 355Asp Ala Asn Ala Ala Phe Phe Asp Asp Leu Gly Ser Pro Gly Gly Ala70 75 80 85gct aag ctt gga cca caa gat ttt gat cac cca atg atc gct gcg ctg 403Ala Lys Leu Gly Pro Gln Asp Phe Asp His Pro Met Ile Ala Ala Leu
90 95 100ccg cct cag gca taatctaacg catgacctct cgc 438Pro Pro Gln Ala
105<210>674<211>105<212>PRT<213>谷氨酸棒杆菌<400>674Met Thr Tyr Thr Ile Ala Gln Pro Cys Val Asp Val Leu Asp Arg Ala1 5 10 15Cys Val Glu Glu Cys Pro Val Asp Cys Ile Tyr Glu Gly Lys Arg Met
20 25 30Leu Tyr Ile His Pro Asp Glu Cys Val Asp Cys Gly Ala Cys Glu Pro
35 40 45Ala Cys Pro Val Glu Ala Ile Phe Tyr Glu Asp Asp Val Pro Asp Glu
50 55 60Trp Leu Asp Tyr Asn Asp Ala Asn Ala Ala Phe Phe Asp Asp Leu Gly65 70 75 80Ser Pro Gly Gly Ala Ala Lys Leu Gly Pro Gln Asp Phe Asp His Pro
85 90 95Met Ile Ala Ala Leu Pro Pro Gln Ala
100 105<210>675<211>441<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(418)<223>RXA00680<400>675ttcttgcatc cccagaagcc gaatacatca ctgggcaaac actcatcgtt gatggtggcc 60gacagttcat ctaagtacta aaagttctaa ggagaagatc atg tct act att cat 115
Met Ser Thr Ile His
1 5ttc att gat cat gct ggc aaa acc cgc acc atc gag gcg act gtt ggt 163Phe Ile Asp His Ala Gly Lys Thr Arg Thr Ile Glu Ala Thr Val Gly
10 15 20gat tca gta atg gag acc gca gtc cga aac gga gtg cct gga att gtt 211Asp Ser Val Met Glu Thr Ala Val Arg Asn Gly Val Pro Gly Ile Val
25 30 35gct gaa tgc ggc ggt tcc tta tcg tgt gca acc tgc cat gtg ttt gtt 259Ala Glu Cys Gly Gly Ser Leu Ser Cys Ala Thr Cys His Val Phe Val
40 45 50gac cct gca cag tat gat gcg ctt ccc cca atg gag gag atg gaa gat 307Asp Pro Ala Gln Tyr Asp Ala Leu Pro Pro Met Glu Glu Met Glu Asp
55 60 65gaa atg ctg tgg ggt gct gcc gtg gac cgt gag gat tgc tcc cgt ttg 355Glu Met Leu Trp Gly Ala Ala Val Asp Arg Glu Asp Cys Ser Arg Leu70 75 80 85tct tgc caa atc aag gtc acc gaa ggc atg gat ctt tcg ttg acc acg 403Ser Cys Gln Ile Lys Val Thr Glu Gly Met Asp Leu Ser Leu Thr Thr
90 95 100cca gaa acg caa gtg tgaggttgaa tcatgaatac ttc 441Pro Glu Thr Gln Val
105<210>676<211>106<212>PRT<213>谷氨酸棒杆菌<400>676Met Ser Thr Ile His Phe Ile Asp His Ala Gly Lys Thr Arg Thr Ile1 5 10 15Glu Ala Thr Val Gly Asp Ser Val Met Glu Thr Ala Val Arg Asn Gly
20 25 30Val Pro Gly Ile Val Ala Glu Cys Gly Gly Ser Leu Ser Cys Ala Thr
35 40 45Cys His Val Phe Val Asp Pro Ala Gln Tyr Asp Ala Leu Pro Pro Met
50 55 60Glu Glu Met Glu Asp Glu Met Leu Trp Gly Ala Ala Val Asp Arg Glu65 70 75 80Asp Cys Ser Arg Leu Ser Cys Gln Ile Lys Val Thr Glu Gly Met Asp
85 90 95Leu Ser Leu Thr Thr Pro Glu Thr Gln Val
100 105<210>677<211>1389<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1366)<223>RXA00679<400>677ggaccgtgag gattgctccc gtttgtcttg ccaaatcaag gtcaccgaag gcatggatct 60ttcgttgacc acgccagaaa cgcaagtgtg aggttgaatc atg aat act tca gct 115
Met Asn Thr Ser Ala
1 5gaa act gga atc ttg atc atc ggt gca aac caa tcg ggt gtg cag ctg 163Glu Thr Gly Ile Leu Ile Ile Gly Ala Asn Gln Ser Gly Val Gln Leu
10 15 20gcg att tcc ctg cgg gcc acg ggt ttc acc gaa tcg atc acg ctt cta 211Ala Ile Ser Leu Arg Ala Thr Gly Phe Thr Glu Ser Ile Thr Leu Leu
25 30 35ggc gag gag gat cac cgc ccc tac cag cgt ccc gcc ttg tcc aag gag 259Gly Glu Glu Asp His Arg Pro Tyr Gln Arg Pro Ala Leu Ser Lys Glu
40 45 50ttc ctc cag gac aag atc gac aaa gag cgt ctg att ttc cgt tcc aat 307Phe Leu Gln Asp Lys Ile Asp Lys Glu Arg Leu Ile Phe Arg Ser Asn
55 60 65gag tat tgg gaa gaa aat aat att cgc ctg gtc aag ggc gtg cgc atc 355Glu Tyr Trp Glu Glu Asn Asn Ile Arg Leu Val Lys Gly Val Arg Ile70 75 80 85gaa cgc att gaa aag aac gac gac gga tca ggg gtc gcc tac ggc gcc 403Glu Arg Ile Glu Lys Asn Asp Asp Gly Ser Gly Val Ala Tyr Gly Ala
90 95 100gga caa gaa ttc gct ttt cga cgt ctc gct cta gcg gtt ggt gcc cgc 451Gly Gln Glu Phe Ala Phe Arg Arg Leu Ala Leu Ala Val Gly Ala Arg
105 110 115cct cgc cac ctc gac ctc ccg ggc gcc acc ttg gag ggt gtc acc tac 499Pro Arg His Leu Asp Leu Pro Ala Thr Leu Glu Gly Val Thr Tyr
120 125 130ctg cgc aac gcg gac gac gcc ttg gcg ctc aaa gcg atg att ggt tct 547Leu Arg Asn Ala Asp Asp Ala Leu Ala Leu Lys Ala Met Ile Gly Ser
135 140 145gtc acc gat gcc gtt gta gtc ggt ggt ggg ttc atc gga ttg gaa gct 595Val Thr Asp Ala Val Val Val Gly Gly Gly Phe Ile Gly Leu Glu Ala150 155 160 165gcg tgt tcg ctt cat gac ctc ggc aaa aat gtc acc gtc ctg gaa tat 643Ala Cys Ser Leu His Asp Leu Gly Lys Asn Val Thr Val Leu Glu Tyr
170 175 180ggt ccg cgt ctg att ggc cga gcg gtg ggt gaa gaa acc gca gca ttc 691Gly Pro Arg Leu Ile Gly Arg AIa Val Gly Glu Glu Thr Ala Ala Phe
185 190 195ttc ctc gaa caa cac cgt tcc cgt ggc gta aat atc gtg ctt gat gcc 739Phe Leu Glu Gln His Arg Ser Arg Gly Val Asn Ile Val Leu Asp Ala
200 205 210cgc atg aaa cag ttt gtg ggc aag gat gga aag ctc agc ggc att gag 787Arg Met Lys Gln Phe Val Gly Lys Asp Gly Lys Leu Ser Gly Ile Glu
215 220 225cta gaa gat ggc aca gta att cct gcc caa cta gtc att gtg ggc atc 835Leu Glu Asp Gly Thr Val Ile Pro Ala Gln Leu Val Ile Val Gly Ile230 235 240 245ggt gtc att ccg aac aca gaa ctt gcc gct gtt ctg ggc tta gac atc 883Gly Val Ile Pro Asn Thr Glu Leu Ala Ala Val Leu Gly Leu Asp Ile
250 255 260aac aac ggc atc gtg gtg gat aaa cat gcc gtc gcg tca gat ggc acc 931Asn Asn Gly Ile Val Val Asp Lys His Ala Val Ala Ser Asp Gly Thr
265 270 275acc att gcg att ggc gat gtc gcc aac att ccc aat cca atc cct ggt 979Thr Ile Ala Ile Gly Asp Val Ala Asn Ile Pro Asn Pro Ile Pro Gly
280 285 290tcc ccc gct gat gaa cgc atc cga cta gaa agc gtc aat aac gcc atc 1027Ser Pro Ala Asp Glu Arg Ile Arg Leu Glu Ser Val Asn Asn Ala Ile
295 300 305gag cac gca aag atc gct gca tac tca ctc gtc ggc cag ccc gaa gcc 1075Glu His Ala Lys Ile Ala Ala Tyr Ser Leu Val Gly Gln Pro Glu Ala310 315 320 325tac gcc gga atc ccc tgg ttc tgg tcc aac caa ggc gat ctc aaa cta 1123Tyr Ala Gly Ile Pro Trp Phe Trp Ser Asn Gln Gly Asp Leu Lys Leu
330 335 340caa att gca gga ctt acc ctt ggt tat gac agc aca gta atc cga cag 1171Gln Ile Ala Gly Leu Thr Leu Gly Tyr Asp Ser Thr Val Ile Arg Gln
345 350 355gat ccc gag aaa aag aag ttc tct gtc ctt tat tac cgt ggc gac aac 1219Asp Pro Glu Lys Lys Lys Phe Ser Val Leu Tyr Tyr Arg Gly Asp Asn
360 365 370atc atc gcc gcc gat tgt gtc aac gct cca ctg gat ttc atg gct gtg 1267Ile Ile Ala Ala Asp Cys Val Asn Ala Pro Leu Asp Phe Met Ala Val
375 380 385cgc agt gca ctt tcc agg aac caa aat atc ccc gcc gac ctt gct gca 1315Arg Ser Ala Leu Ser Arg Asn Gln Asn Ile Pro Ala Asp Leu Ala Ala390 395 400 405gat att tcg cag ccg ctg aaa aaa cta gcc gtt gac ctg gag gtt acc 1363Asp Ile Ser Gln Pro Leu Lys Lys Leu Ala Val Asp Leu Glu Val Thr
410 415 420cga tgactcgcag taatttaccc gct 1389Arg<210>678<211>422<212>PRT<213>谷氨酸棒杆菌<400>678Met Asn Thr Ser Ala Glu Thr Gly Ile Leu Ile Ile Gly Ala Asn Gln1 5 10 15Ser Gly Val Gln Leu Ala Ile Ser Leu Arg Ala Thr Gly Phe Thr Glu
20 25 30Ser Ile Thr Leu Leu Gly Glu Glu Asp His Arg Pro Tyr Gln Arg Pro
35 40 45Ala Leu Ser Lys Glu Phe Leu Gln Asp Lys Ile Asp Lys Glu Arg Leu
50 55 60Ile Phe Arg Ser Asn Glu Tyr Trp Glu Glu Asn Asn Ile Arg Leu Val65 70 75 80Lys Gly Val Arg Ile Glu Arg Ile Glu Lys Asn Asp Asp Gly Ser Gly
85 90 95Val Ala Tyr Gly Ala Gly Gln Glu Phe Ala Phe Arg Arg Leu Ala Leu
100 105 110Ala Val Gly Ala Arg Pro Arg His Leu Asp Leu Pro Gly Ala Thr Leu
115 120 125Glu Gly Val Thr Tyr Leu Arg Asn Ala Asp Asp Ala Leu Ala Leu Lys
130 135 140Ala Met Ile Gly Ser Val Thr Asp Ala Val Val Val Gly Gly Gly Phe145 150 155 160Ile Gly Leu Glu Ala Ala Cys Ser Leu His Asp Leu Gly Lys Asn Val
165 170 175Thr Val Leu Glu Tyr Gly Pro Arg Leu Ile Gly Arg Ala Val Gly Glu
180 185 190Glu Thr Ala Ala Phe Phe Leu Glu Gln His Arg Ser Arg Gly Val Asn
195 200 205Ile Val Leu Asp Ala Arg Met Lys Gln Phe Val Gly Lys Asp Gly Lys
210 215 220Leu Ser Gly Ile Glu Leu Glu Asp Gly Thr Val Ile Pro Ala Gln Leu225 230 235 240Val Ile Val Gly Ile Gly Val Ile Pro Asn Thr Glu Leu Ala Ala Val
245 250 255Leu Gly Leu Asp Ile Asn Asn Gly Ile Val Val Asp Lys His Ala Val
260 265 270Ala Ser Asp Gly Thr Thr Ile Ala Ile Gly Asp Val Ala Asn Ile Pro
275 280 285Asn Pro Ile Pro Gly Ser Pro Ala Asp Glu Arg Ile Arg Leu Glu Ser
290 295 300Val Asn Asn Ala Ile Glu His Ala Lys Ile Ala Ala Tyr Ser Leu Val305 310 315 320Gly Gln Pro Glu Ala Tyr Ala Gly Ile Pro Trp Phe Trp Ser Asn Gln
325 330 335Gly Asp Leu Lys Leu Gln Ile Ala Gly Leu Thr Leu Gly Tyr Asp Ser
340 345 350Thr Val Ile Arg Gln Asp Pro Glu Lys Lys Lys Phe Ser Val Leu Tyr
355 360 365Tyr Arg Gly Asp Asn Ile Ile Ala Ala Asp Cys Val Asn Ala Pro Leu
370 375 380Asp Phe Met Ala Val Arg Ser Ala Leu Ser Arg Asn Gln Asn Ile Pro
390 395 400Ala Asp Leu Ala Ala Asp Ile Ser Gln Pro Leu Lys Lys Leu Ala Val
405 410 415Asp Leu Glu Val Thr Arg
420<210>679<211>1074<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1051)<223>RXA00224<400>679gtgatgtcat tggtgcatcg ggtgctgctg aaaagattgc tgagtacctc gcttcagaga 60acctcatcta gccactatct tcacaaagga gaacatttaa atg tct att tct tat 115
Met Ser Ile Ser Tyr
1 5gtg ctg gtt gag cag cta gat ggc cgc cca gaa cca gtt acc ctt gaa 163Val Leu Val Glu Gln Leu Asp Gly Arg Pro Glu Pro Val Thr Leu Glu
10 15 20ttg atc act gct gct cgc gca ctc ggt gac gtc gtt gcc gtt gtc gtt 211Leu Ile Thr Ala Ala Arg Ala Leu Gly Asp Val Val Ala Val Val Val
25 30 35ggc gag cca ggt gcc ggc gta aac ctt gct gct gag ctc ggc aat tgg 259Gly Glu Pro Gly Ala Gly Val Asn Leu Ala Ala Glu Leu Gly Asn Trp
40 45 50ggt gca gca cag gtt gtt tcc gct gaa atc tct ggc gct tcc aac cgt 307Gly Ala Ala Gln Val Val Ser Ala Glu Ile Ser Gly Ala Ser Asn Arg
55 60 65ttg atc ttg cct gct gtt gat gcg ctg cac att ttg gct gcg aac aac 355Leu Ile Leu Pro Ala Val Asp Ala Leu His Ile Leu Ala Ala Asn Asn70 75 80 85cca ggt cca att gtt atc gct gca act gca agc ggt aat gag atc gct 403Pro Gly Pro Ile Val Ile Ala Ala Thr Ala Ser Gly Asn Glu Ile Ala
90 95 100ggt cgt ttg gct gcc cgt ttg gct tct ggt gtg ctc acc gat gtc gtc 451Gly Arg Leu Ala Ala Arg Leu Ala Ser Gly Val Leu Thr Asp Val Val
105 110 115gga atc aat gcc gac cgc acc gca cag cag tcc att ttc ggc gac acc 499Gly Ile Asn Ala Asp Arg Thr Ala Gln Gln Ser Ile Phe Gly Asp Thr
120 125 130att cag gtg tcc gct gca gtt ggt ggc gct tca ccg ctg tac acc ctg 547Ile Gln Val Ser Ala Ala Val Gly Gly Ala Ser Pro Leu Tyr Thr Leu
135 140 145cgt cca ggt gcc ctt gat ggc gtg gcc gtt cct gca acc ggt gaa ttg 595Arg Pro Gly Ala Leu Asp Gly Val Ala Val Pro Ala Thr Gly Glu Leu150 155 160 165gca acc att gag atc cca ggc gca acc gcc aag gat gtc acc atc acc 643Ala Thr Ile Glu Ile Pro Gly Ala Thr Ala Lys Asp Val Thr Ile Thr
170 175 180tcc ttc acg cca agc acc cag agc gat cgc cct gag ctg cca cag gca 691Ser Phe Thr Pro Ser Thr Gln Ser Asp Arg Pro Glu Leu Pro Gln Ala
185 190 195aag gtc gtt atc gca ggt gga cgt ggt gtc gga agc gaa gaa aac ttc 739Lys Val Val Ile Ala Gly Gly Arg Gly Val Gly Ser Glu Glu Asn Phe
200 205 210cgc aac atc gtt gaa cca ctg gca gat gca ttg ggc ggt gcc gtt ggc 787Arg Ser Ile Val Glu Pro Leu Ala Asp Ala Leu Gly Gly Ala Val Gly
215 220 225gca acc cgc gac gcc gtt gat ctg ggc tac tac cca ggc gag tac cag 835Ala Thr Arg Asp Ala Val Asp Leu Gly Tyr Tyr Pro Gly Glu Tyr Gln230 235 240 245gtt ggt cag acc ggt gtc acc gtg tcc cca gac ctc tac atc ggc ctc 883Val Gly Gln Thr Gly Val Thr Val Ser Pro Asp Leu Tyr Ile Gly Leu
250 255 260ggc att tcc ggt gca att cag cac act tct ggt atg cag acc gca aag 931Gly Ile Ser Gly Ala Ile Gln His Thr Ser Gly Met Gln Thr Ala Lys
265 270 275aag gtt att gtg atc aac aac gat gag gac gcg ccg atc ttc cag att 979Lys Val Ile Val Ile Asn Asn Asp Glu Asp Ala Pro Ile Phe Gln Ile
280 285 290gcg gac ctc ggt gtc gtt ggc gac ctc ttt gac atc gcc cct gcg ctc 1027Ala Asp Leu Gly Val Val Gly Asp Leu Phe Asp Ile Ala Pro Ala Leu
295 300 305atc gaa gag atc aac aag cgc aag taggagtttt gaacactttt tat 1074Ile Glu Glu Ile Asn Lys Arg Lys310 315<210>680<211>317<212>PRT<213>谷氨酸棒杆菌<400>680Met Ser Ile Ser Tyr Val Leu Val Glu Gln Leu Asp Gly Arg Pro Glu1 5 10 15Pro Val Thr Leu Glu Leu Ile Thr Ala Ala Arg Ala Leu Gly Asp Val
20 25 30Val Ala Val Val Val Gly Glu Pro Gly Ala Gly Val Asn Leu Ala Ala
35 40 45Glu Leu Gly Asn Trp Gly Ala Ala Gln Val Val Ser Ala Glu Ile Ser
50 55 60Gly Ala Ser Asn Arg Leu Ile Leu Pro Ala Val Asp Ala Leu His Ile65 70 75 80Leu Ala Ala Asn Asn Pro Gly Pro Ile Val Ile Ala Ala Thr Ala Ser
85 90 95Gly Asn Glu Ile Ala Gly Arg Leu Ala Ala Arg Leu Ala Ser Gly Val
100 105 110Leu Thr Asp Val Val Gly Ile Asn Ala Asp Arg Thr Ala Gln Gln Ser
115 120 125Ile Phe Gly Asp Thr Ile Gln Val Ser Ala Ala Val Gly Gly Ala Ser
130 135 140Pro Leu Tyr Thr Leu Arg Pro Gly Ala Leu Asp Gly Val Ala Val Pro145 150 155 160Ala Thr Gly Glu Leu Ala Thr Ile Glu Ile Pro Gly Ala Thr Ala Lys
165 170 175Asp Val Thr Ile Thr Ser Phe Thr Pro Ser Thr Gln Ser Asp Arg Pro
180 185 190Glu Leu Pro Gln Ala Lys Val Val Ile Ala Gly Gly Arg Gly Val Gly
195 200 205Ser Glu Glu Asn Phe Arg Ser Ile Val Glu Pro Leu Ala Asp Ala Leu
210 215 220Gly Gly Ala Val Gly Ala Thr Arg Asp Ala Val Asp Leu Gly Tyr Tyr225 230 235 240Pro Gly Glu Tyr Gln Val Gly Gln Thr Gly Val Thr Val Ser Pro Asp
245 250 255Leu Tyr Ile Gly Leu Gly Ile Ser Gly Ala Ile Gln His Thr Ser Gly
260 265 270Met Gln Thr Ala Lys Lys Val Ile Val Ile Asn Asn Asp Glu Asp Ala
275 280 285Pro Ile Phe Gln Ile Ala Asp Leu Gly Val Val Gly Asp Leu Phe Asp
290 295 300Ile Ala Pro Ala Leu Ile Glu Glu Ile Asn Lys Arg Lys305 310 315<210>681<211>909<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(886)<223>RXA00225<400>681gtaggcgtcg aaaagcaatg ggcgaagccc gcgtagtatg ggcgggcaac gctaaaagcg 60ccaaaaacgc caaaaatcgt gaattgaaag gtgagtgtgg atg tcc aca atc gtg 115
Met Ser Thr Ile Val
1 5gtt ctg gtt aaa aat gtt cca gac acc tgg tct aag agg act ctg gaa 163Val Leu Val Lys Asn Val Pro Asp Thr Trp Ser Lys Arg Thr Leu Glu
10 15 20gct gat ttc acc ctt gac cgt gag ggt gta gat cga gtc ttg gat gag 211Ala Asp Phe Thr Leu Asp Arg Glu Gly Val Asp Arg Val Leu Asp Glu
25 30 35atc aat gag ttt gct ctg gag cag gca ctg cgc ttg cgg gag tcc aac 259Ile Asn Glu Phe Ala Leu Glu Gln Ala Leu Arg Leu Arg Glu Ser Asn
40 45 50ccg gat gct ggt tac cgc gtt gtt gcg ctg agc gcc ggc cct gcc ggt 307Pro Asp Ala Gly Tyr Arg Val Val Ala Leu Ser Ala Gly Pro Ala Gly
55 60 65ggg gaa gag gcg ctg cgt aag gcg ctg tcc atg ggt gct gat gaa gca 355Gly Glu Glu Ala Leu Arg Lys Ala Leu Ser Met Gly Ala Asp Glu Ala70 75 80 85atc cag ctc agt gat gat gcc ttg gct ggt tct gat ctt ttg gga acc 403Ile Gln Leu Ser Asp Asp Ala Leu Ala Gly Ser Asp Leu Leu Gly Thr
90 95 100gct tgg gcg ctg aac aac gct atc aac acc atc gcg ggt gtt gct ctc 451Ala Trp Ala Leu Asn Asn Ala 1le Asn Thr Ile Ala Gly Val Ala Leu
105 110 115atc gtg acg ggt tcg gct tct tcc gat ggt tcc atg ggt gcg ctt cct 499Ile Val Thr Gly Ser Ala Ser Ser Asp Gly Ser Met Gly Ala Leu Pro
120 125 130ggc gtg tta gct gag tac cgc cag gtc cca gcg ttg act aac ttg tct 547Gly Val Leu Ala Glu Tyr Arg Gln Val Pro Ala Leu Thr Asn Leu Ser
135 140 145gcg ctg aag gtc gag ggt gca tct att act gcc act cgc att gat aac 595Ala Leu Lys Val Glu Gly Ala Ser Ile Thr Ala Thr Arg Ile Asp Asn150 155 160 165cac ggc acc tat gag ttg cag gct gca ctt cct gcg gtt gtg tcg att 643His Gly Thr Tyr Glu Leu Gln Ala Ala Leu Pro Ala Val Val Ser Ile
170 175 180tcc gat aag gct gac aag cca cgt ttc cct aac ttc aag ggc atc atg 691Ser Asp Lys Ala Asp Lys Pro Arg Phe Pro Asn Phe Lys Gly Ile Met
185 190 195gct gct aag aag gct gag atc aag aag ctt tcc ttg gct gaa atc ggc 739Ala Ala Lys Lys Ala Glu Ile Lys Lys Leu Ser Leu Ala Glu Ile Gly
200 205 210gtg gct cca gag cag gtt ggt ctg tct cac gcg gca act gct gtt act 787Val Ala Pro Glu Gln Val Gly Leu Ser His Ala Ala Thr Ala Val Thr
215 220 225gct gca gct gat cgt cct gag cgc tcc caa ggt gat gtc att ggt gca 835Ala Ala Ala Asp Arg Pro Glu Arg Ser Gln Gly Asp Val Ile Gly Ala230 235 240 245tcg ggt gct gct gaa aag att gct gag tac ctc gct tca gag aac ctc 883Ser Gly Ala Ala Glu Lys Ile Ala Glu Tyr Leu Ala Ser Glu Asn Leu
250 255 260atc tagccactat cttcacaaag gag 909Ile<210>682<211>262<212>PRT<213>谷氨酸棒杆菌<400>682Met Ser Thr Ile Val Val Leu Val Lys Asn Val Pro Asp Thr Trp Ser1 5 10 15Lys Arg Thr Leu Glu Ala Asp Phe Thr Leu Asp Arg Glu Gly Val Asp
20 25 30Arg Val Leu Asp Glu Ile Asn Glu Phe Ala Leu Glu Gln Ala Leu Arg
35 40 45Leu Arg Glu Ser Asn Pro Asp Ala Gly Tyr Arg Val Val Ala Leu Ser
50 55 60Ala Gly Pro Ala Gly Gly Glu Glu Ala Leu Arg Lys Ala Leu Ser Met65 70 75 80Gly Ala Asp Glu Ala Ile Gln Leu Ser Asp Asp Ala Leu Ala Gly Ser
85 90 95Asp Leu Leu Gly Thr Ala Trp Ala Leu Asn Asn Ala Ile Asn Thr Ile
100 105 110Ala Gly Val Ala Leu Ile Val Thr Gly Ser Ala Ser Ser Asp Gly Ser
115 120 125Met Gly Ala Leu Pro Gly Val Leu Ala Glu Tyr Arg Gln Val Pro Ala
130 135 140Leu Thr Asn Leu Ser Ala Leu Lys Val Glu Gly Ala Sar Ile Thr Ala145 150 155 160Thr Arg Ile Asp Asn His Gly Thr Tyr Glu Leu Gln Ala Ala Leu Pro
165 170 175Ala Val Val Ser Ile Ser Asp Lys Ala Asp Lys Pro Arg Phe Pro Asn
180 185 190Phe Lys Gly Ile Met Ala Ala Lys Lys Ala Glu Ile Lys Lys Leu Ser
195 200 205Leu Ala Glu Ile Gly Val Ala Pro Glu Gln Val Gly Leu Ser His Ala
210 215 220Ala Thr Ala Val Thr Ala Ala Ala Asp Arg Pro Glu Arg Ser Gln Gly225 230 235 240Asp Val Ile Gly Ala Ser Gly Ala Ala Glu Lys Ile Ala Glu Tyr Leu
245 250 255Ala Ser Glu Asn Leu Ile
260<210>683<211>2378<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(82)..(2355)<223>RXN00606<400>683tgcggtggcg attgctgcga cccaagctgg caccaccagc ctcgatggta ttttgcactc 60tgatttctgg cggagaagcc a gtg ctc acg ggt gtt att gcg gtg ctg att 111
Val Leu Thr Gly Val Ile Ala Val Leu Ile
1 5 10gca atg tcc gcg ttc act aag tcc gca cag ttc ccg ttc cac ttc tgg 159Ala Met Ser Ala Phe Thr Lys Ser Ala Gln Phe Pro Phe His Phe Trp
15 20 25ctg cct gag gcg atg gct gcg gcc acc cca gtg tcg gcg ttc ctg cac 207Leu Pro Glu Ala Met Ala Ala Ala Thr Pro Val Ser Ala Phe Leu His
30 35 40gct gcg gcc gtg gtc aag gcg ggt att tac ctg ttg ctg cgc ttt agc 255Ala Ala Ala Val Val Lys Ala Gly Ile Tyr Leu Leu Leu Arg Phe Ser
45 50 55att gtg ttc cat gat gtt gcg gtc tgg aat tgg ttg ctg att atc gtc 303Ile Val Phe His Asp Val Ala Val Trp Asn Trp Leu Leu Ile Ile Val
60 65 70ggc atg ggt acg gcc atc atg tcg gcg tat ttc gcg gtg cag aag acc 351Gly Met Gly Thr Ala Ile Met 5er Ala Tyr Phe Ala Val Gln Lys Thr75 80 85 90gat ctg aag aag ctc acg gca tat tcc acg gtg tcg cat ttg ggt tgg 399Asp Leu Lys Lys Leu Thr Ala Tyr Ser Thr Val Ser His Leu Gly Trp
95 100 105atc gta gcg acc atc ggc gtg ggc act cct ttc gcg ctc ggc gct gcc 447Ile Val Ala Thr Ile Gly Val Gly Thr Pro Phe Ala Leu Gly Ala Ala
110 115 120att gtg cac acg ctc agc cac gcg ctg ttt aag tcc tcg ttg ttc atg 495Ile Val His Thr Leu Ser His Ala Leu Phe Lys Ser Ser Leu Phe Met
125 130 135ctc att ggc gtg att gat cac cag act ggc acg cgc gat att cgt cgc 543Leu Ile Gly Val Ile Asp His Gln Thr Gly Thr Arg Asp Ile Arg Arg
140 145 150ctc ggt ttc ctg gtc aag aag atg ccg ttc acg ttt gtg tct gta tta 591Leu Gly Phe Leu Val Lys Lys Met Pro Phe Thr Phe Val Ser Val Leu155 160 165 170ata ggt gcg ttg tcg atg gca tcg gtt ccg ccg ttg ctc ggc ttc gtg 639Ile Gly Ala Leu Ser Met Ala Ser Val Pro Pro Leu Leu Gly Phe Val
175 180 185tcc aaa gaa ggc atg atc aca gcg ttc atg gac gcc ccc atc ggc aac 687Ser Lys Glu Gly Met Ile Thr Ala Phe Met Asp Ala Pro Ile Gly Asn
190 195 200tcc tat gtt gta tta ctg ctg gtc ggc gca gca atc ggc gcg gtc cta 735Ser Tyr Val Val Leu Leu Leu Val Gly Ala Ala Ile Gly Ala Val Leu
205 210 215acc ttc aca tac tcc gcg aaa ctc gtg ctc ggc gca ttc gtc gac ggc 783Thr Phe Thr Tyr Ser Ala Lys Leu Val Leu Gly Ala Phe Val Asp Gly
220 225 230cca cgc gac atg tca cac gtc aag gaa gcc ccc gtc tcc ctc tgg ctt 831Pro Arg Asp Met Ser His Val Lys Glu Ala Pro Val Ser Leu Trp Leu235 240 245 250ccg gcc gcc ctg cct gga ctt atg tct ctg cca cta gtc cta gta ctt 879Pro Ala Ala Leu Pro Gly Leu Met Ser Leu Pro Leu Val Leu Val Leu
255 260 265tcg ctt ttc gac gcc ccc gtc tcc gcc gca gcc acc tcc gcc gcg ggg 927Ser Leu Phe Asp Ala Pro Val Ser Ala Ala Ala Thr Ser Ala Ala Gly
270 275 280gaa gcg gcg cac atg cac ctg gca ttg tgg cac ggc atc aac acc cca 975Glu Ala Ala His Met His Leu Ala Leu Trp His Gly Ile Asn Thr Pro
285 290 295ctg ttg att tcc ttg ggt gtg ctg gtg gcc gga atc ctt ggt gtg ctg 1023Leu Leu Ile Ser Leu Gly Val Leu Val Ala Gly Ile Leu Gly Val Leu
300 305 310ttc cgc aaa gag ctg tgg aaa atc gcc gag acc agc cct ttc ccc atc 1071Phe Arg Lys Glu Leu Trp Lys Ile Ala Glu Thr Ser Pro Phe Pro Ile315 320 325 330gcc aca ggc aac gac atc cta tcg atg ctg gtt tac cga gcc aac ttg 1119Ala Thr Gly Asn Asp Ile Leu Ser Met Leu Val Tyr Arg Ala Asn Leu
335 340 345ctg ggt aaa ttc ttc ggt cgc atg gct gat tcg atg agc cca cgc agg 1167Leu Gly Lys Phe Phe Gly Arg Met Ala Asp Ser Met Ser Pro Arg Arg
350 355 350cac ttg gtc agc ctc atc gtg ctg ctc tgg gcg ctg gct gct ttt gcc 1215His Leu Val Ser Leu Ile Val Leu Leu Trp Ala Leu Ala Ala Phe Ala
365 370 375acc att cac ccc tcg gtt cag ctt gca cca aag caa ccg gga att gat 1263Thr Ile His Pro Ser Val Gln Leu Ala Pro Lys Gln Pro Gly Ile Asp
380 385 390cgt tgg atc gac ctc att ccg ctt gcc atc atc gcg cta tct gtc ttc 1311Arg Trp Ile Asp Leu Ile Pro Leu Ala Ile Ile Ala Leu Ser Val Phe395 400 405 410ggc ctg ctc acc acc cga aac cgc ctc agc gca gcc gtg ctt gtg ggt 1359Gly Leu Leu Thr Thr Arg Asn Arg Leu Ser Ala Ala Val Leu Val Gly
415 420 425acc gtt ggt gtg ggt gtt tcc ttc cag atg cta ctt ctg ggc gct ccc 1407Thr Val Gly Val Gly Val Ser Phe Gln Met Leu Leu Leu Gly Ala Pro
430 435 440gat gtt gca ctt acc cag ttc ctg gta gaa ggc ctc gtc gtg gta atc 1455Asp Val Ala Leu Thr Gln Phe Leu Val Glu Gly Leu Val Val Val Ile
445 450 455atc atg atg gtt gtc cgg cac cag cct gcc aac ttc aag cgc atc aag 1503Ile Met Met Val Val Arg His Gln Pro Ala Asn Phe Lys Arg Ile Lys
460 465 470ccc agc aga agg cgc agc acc gtt ctt gtc gcc gtc ctt gct gcc ttc 1551Pro Ser Arg Arg Arg Ser Thr Val Leu Val Ala Val Leu Ala Ala Phe475 480 485 490gcc gca ttc atg gcg gtg tgg gga ttg ctt ggc cgt cac gaa cgt tct 1599Ala Ala Phe Met Ala Val Trp Gly Leu Leu Gly Arg His Glu Arg Ser
495 500 505gag ctg gcc atg tgg tac ctc aac caa ggt cca gag atc acc tct ggc 1647Glu Leu Ala Met Trp Tyr Leu Asn Gln Gly Pro Glu Ile Thr Ser Gly
510 515 520gcc aac gtg gtg aac acc atc ctc gtg gaa ttc cgt gca ctg gat acg 1695Ala Asn Val Val Asn Thr Ile Leu Val Glu Phe Arg Ala Leu Asp Thr
525 530 535ttg ggc gag ctc tcc gtg ctt ggc atg gca gct gtc gtc atc ggt gcg 1743Leu Gly Glu Leu Ser Val Leu Gly Met Ala Ala Val Val Ile Gly Ala
540 545 550atg gtg gct tcc atg cct cgt cat ccg ttt gcc aag ggc acc cac cct 1791Met Val Ala Ser Met Pro Arg His Pro Phe Ala Lys Gly Thr His Pro555 560 565 570cgc ccc ttt ggc caa tca cag ttg aac tcc att ccg ctg cgc atg ctg 1839Arg Pro Phe Gly Gln Ser Gln Leu Asn Ser Ile Pro Leu Arg Met Leu
575 580 585ctt aag gtg ctg gtt cca gcg cta tgc ttc ttg agc ttc atg gtg ttc 1887Leu Lys Val Leu Val Pro Ala Leu Cys Phe Leu Ser Phe Met Val Phe
590 595 600atg cgt gga cac aat gat ccg gga ggc ggt ttc atc gca gcc cta att 1935Met Arg Gly His Asn Asp Pro Gly Gly Gly Phe Ile Ala Ala Leu Ile
605 610 615gcc ggt ggc gcg ctg atg ctc ctg tac ctg tcc aag gcc aaa gat ggc 1983Ala Gly Gly Ala Leu Met Leu Leu Tyr Leu Ser Lys Ala Lys Asp Gly
620 625 630cgc att ttc cgc ccg aat gtt cct ttc att ctc act ggt gcg ggc atc 2031Arg Ile Phe Arg Pro Asn Val Pro Phe Ile Leu Thr Gly Ala Gly Ile635 640 645 650ttg atg gca gtg ttc tcg ggc gta ctg gga ctc acc cac ggt tct ttc 2079Leu Met Ala Val Phe Ser Gly Val Leu Gly Leu Thr His Gly Ser Phe
655 660 665ctg tac gcc atc cac ttc aac ttc gta ggc cag cac tgg acc acc tcg 2127Leu Tyr Ala Ile His Phe Asn Phe Val Gly Gln His Trp Thr Thr Ser
670 675 680atg atc ttc gac ctc ggc gtg tac ctg gcc gtg ttg ggc atg gtg tcc 2175Met Ile Phe Asp Leu Gly Val Tyr Leu Ala Val Leu Gly Met Val Ser
685 690 695atg gca atc aac ggc ctg ggc gga tac ctg cgc cca ggt acc gac aat 2223Met Ala Ile Asn Gly Leu Gly Gly Tyr Leu Arg Pro Gly Thr Asp Asn
700 705 710gca gat ctg gac tac gcc cgc cga agt ggc cca ctg cca gca acg cca 2271Ala Asp Leu Asp Tyr Ala Arg Arg Ser Gly Pro Leu Pro Ala Thr Pro715 720 725 730acg gtt gaa ccc gaa cca gaa ggc gat gaa gac tgg ccc gaa ccc atc 2319Thr Val Glu Pro Glu Pro Glu Gly Asp Glu Asp Trp Pro Glu Pro Ile
735 740 745aac ccc gca ggc gat aac aaa gag gag gca aac cga tgattctcgc 2365Asn Pro Ala Gly Asp Asn Lys Glu Glu Ala Asn Arg
750 755actgacagtc gcg 2378<210>684<211>758<212>PRT<213>谷氨酸棒杆菌<400>684Val Leu Thr Gly Val Ile Ala Val Leu Ile Ala Met Ser Ala Phe Thr1 5 10 15Lys Ser Ala Gln Phe Pro Phe His Phe Trp Leu Pro Glu Ala Met Ala
20 25 30Ala Ala Thr Pro Val Ser Ala Phe Leu His Ala Ala Ala Val Val Lys
35 40 45Ala Gly Ile Tyr Leu Leu Leu Arg Phe Ser Ile Val Phe His Asp Val
50 55 60Ala Val Trp Asn Trp Leu Leu Ile Ile Val Gly Met Gly Thr Ala Ile65 70 75 80Met Ser Ala Tyr Phe Ala Val Gln Lys Thr Asp Leu Lys Lys Leu Thr
85 90 95Ala Tyr Ser Thr Val Ser His Leu Gly Trp Ile Val Ala Thr Ile Gly
100 105 110Val Gly Thr Pro Phe Ala Leu Gly Ala Ala Ile Val His Thr Leu Ser
115 120 125His Ala Leu Phe Lys Ser Ser Leu Phe Met Leu Ile Gly Val Ile Asp
130 135 140His Gln Thr Gly Thr Arg Asp Ile Arg Arg Leu Gly Phe Leu Val Lys145 150 155 160Lys Met Pro Phe Thr Phe Val Ser Val Leu Ile Gly Ala Leu Ser Met
165 170 175Ala Ser Val Pro Pro Leu Leu Gly Phe Val Ser Lys Glu Gly Met Ile
180 185 190Thr Ala Phe Met Asp Ala Pro Ile Gly Asn Ser Tyr Val Val Leu Leu
195 200 205Leu Val Gly Ala Ala Ile Gly Ala Val Leu Thr Phe Thr Tyr Ser Ala
210 215 220Lys Leu Val Leu Gly Ala Phe Val Asp Gly Pro Arg Asp Met Ser His225 230 235 240Val Lys Glu Ala Pro Val Ser Leu Trp Leu Pro Ala Ala Leu Pro Gly
245 250 255Leu Met Ser Leu Pro Leu Val Leu Val Leu Ser Leu Phe Asp Ala Pro
260 265 270Val Ser Ala Ala Ala Thr Ser Ala Ala Gly Glu Ala Ala His Met His
275 280 285Leu Ala Leu Trp His Gly Ile Asn Thr Pro Leu Leu Ile Ser Leu Gly
290 295 300Val Leu Val Ala Gly Ile Leu Gly Val Leu Phe Arg Lys Glu Leu Trp305 310 315 320Lys Ile Ala Glu Thr Ser Pro Phe Pro Ile Ala Thr Gly Asn Asp Ile
325 330 335Leu Ser Met Leu Val Tyr Arg Ala Asn Leu Leu Gly Lys Phe Phe Gly
340 345 350Arg Met Ala Asp Ser Met Ser Pro Arg Arg His Leu Val Ser Leu Ile
355 360 365Val Leu Leu Trp Ala Leu Ala Ala Phe Ala Thr Ile His Pro Ser Val
370 375 380Gln Leu Ala Pro Lys Gln Pro Gly Ile Asp Arg Trp Ile Asp Leu Ile385 390 395 400Pro Leu Ala Ile Ile Ala Leu Ser Val Phe Gly Leu Leu Thr Thr Arg
405 410 415Asn Arg Leu Ser Ala Ala Val Leu Val Gly Thr Val Gly Val Gly Val
420 425 430Ser Phe Gln Met Leu Leu Leu Gly Ala Pro Asp Val Ala Leu Thr Gln
435 440 445Phe Leu Val Glu Gly Leu Val Val Val Ile Ile Met Met Val Val Arg
450 455 460His Gln Pro Ala Asn Phe Lys Arg Ile Lys Pro Ser Arg Arg Arg Ser465 470 475 480Thr Val Leu Val Ala Val Leu Ala Ala Phe Ala Ala Phe Met Ala Val
485 490 495Trp Gly Leu Leu Gly Arg His Glu Arg Ser Glu Leu Ala Met Trp Tyr
500 505 510Leu Asn Gln Gly Pro Glu Ile Thr Ser Gly Ala Asn Val Val Asn Thr
515 520 525Ile Leu Val Glu Phe Arg Ala Leu Asp Thr Leu Gly Glu Leu Ser Val
530 535 540Leu Gly Met Ala Ala Val Val Ile Gly Ala Met Val Ala Ser Met Pro545 550 555 560Arg His Pro Phe Ala Lys Gly Thr His Pro Arg Pro Phe Gly Gln Ser
565 570 575Gln Leu Asn Ser Ile Pro Leu Arg Met Leu Leu Lys Val Leu Val Pro
580 585 590Ala Leu Cys Phe Leu Ser Phe Met Val Phe Met Arg Gly His Asn Asp
595 600 605Pro Gly Gly Gly Phe Ile Ala Ala Leu Ile Ala Gly Gly Ala Leu Met
610 615 620Leu Leu Tyr Leu Ser Lys Ala Lys Asp Gly Arg Ile Phe Arg Pro Asn625 630 635 640Val Pro Phe Ile Leu Thr Gly Ala Gly Ile Leu Met Ala Val Phe Ser
645 650 655Gly Val Leu Gly Leu Thr His Gly Ser Phe Leu Tyr Ala Ile His Phe
660 665 670Asn Phe Val Gly Gln His Trp Thr Thr Ser Met Ile Phe Asp Leu Gly
675 680 685Val Tyr Leu Ala Val Leu Gly Met Val Ser Met Ala Ile Asn Gly Leu
690 695 700Gly Gly Tyr Leu Arg Pro Gly Thr Asp Asn Ala Asp Leu Asp Tyr Ala705 710 715 720Arg Arg Ser Gly Pro Leu Pro Ala Thr Pro Thr Val Glu Pro Glu Pro
725 730 735Glu Gly Asp Glu Asp Trp Pro Glu Pro Ile Asn Pro Ala Gly Asp Asn
740 745 750Lys Glu Glu Ala Asn Arg
755<210>685<211>1872<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1849)<223>FRXA00606<400>685atggataacc agactggcac gcgcgatatt cgtcgcctcg gtttcctggt caagaaaaat 60gccgttcacg tttgtgtctg tattaatagg tgcgttgtcg atg gca tcg gtt ccg 115
Met Ala Ser Val Pro
1 5ccg ttg ctc ggc ttc gtg tcc aaa gaa ggc atg atc aca gcg ttc atg 163Pro Leu Leu Gly Phe Val Ser Lys Glu Gly Met Ile Thr Ala Phe Met
10 15 20gac gcc ccc atc ggc aac tcc tat gtt gta tta ctg ctg gtc ggc gca 211Asp Ala Pro Ile Gly Asn Ser Tyr Val Val Leu Leu Leu Val Gly Ala
25 30 35gca atc ggc gcg gtc cta acc ttc aca tac tcc gcg aaa ctc gtg ctc 259Ala Ile Gly Ala Val Leu Thr Phe Thr Tyr Ser Ala Lys Leu Val Leu
40 45 50ggc gca ttc gtc gac ggc cca cgc gac atg tca cac gtc aag gaa gcc 307Gly Ala Phe Val Asp Gly Pro Arg Asp Met Ser His Val Lys Glu Ala
55 60 65ccc gtc tcc ctc tgg ctt ccg gcc gcc ctg cct gga ctt atg tct ctg 355Pro Val Ser Leu Trp Leu Pro Ala Ala Leu Pro Gly Leu Met Ser Leu70 75 80 85cca cta gtc cta gta ctt tcg ctt ttc gac gcc ccc gtc tcc gcc gca 403Pro Leu Val Leu Val Leu Ser Leu Phe Asp Ala Pro Val Ser Ala Ala
90 95 100gcc acc tcc gcc gcg ggg gaa gcg gcg cac atg cac ctg gca ttg tgg 451Ala Thr Ser Ala Ala Gly Glu Ala Ala His Met His Leu Ala Leu Trp
105 110 115cac ggc atc aac acc cca ctg ttg att tcc ttg ggt gtg ctg gtg gcc 499His Gly Ile Asn Thr Pro Leu Leu Ile Ser Leu Gly Val Leu Val Ala
120 125 130gga atc ctt ggt gtg ctg ttc cgc aaa gag ctg tgg aaa atc gcc gag 547Gly Ile Leu Gly Val Leu Phe Arg Lys Glu Leu Trp Lys Ile Ala Glu
135 140 145acc agc cct ttc ccc atc gcc aca ggc aac gac atc cta tcg atg ctg 595Thr Ser Pro Phe Pro Ile Ala Thr Gly Asn Asp Ile Leu Ser Met Leu150 155 160 165gtt tac cga gcc aac ttg ctg ggt aaa ttc ttc ggt cgc atg gct gat 643Val Tyr Arg Ala Asn Leu Leu Gly Lys Phe Phe Gly Arg Met Ala Asp
170 175 180tcg atg agc cca cgc agg cac ttg gtc agc ctc atc gtg ctg ctc tgg 691Ser Met Ser Pro Arg Arg His Leu Val Ser Leu Ile Val Leu Leu Trp
185 190 195gcg ctg gct gct ttt gcc acc att cac ccc tcg gtt cag ctt gca cca 739Ala Leu Ala Ala Phe Ala Thr Ile His Pro Ser Val Gln Leu Ala Pro
200 205 210aag caa ccg gga att gat cgt tgg atc gac ctc att ccg ctt gcc atc 787Lys Gln Pro Gly Ile Asp Arg Trp Ile Asp Leu Ile Pro Leu Ala Ile
215 220 225atc gcg cta tct gtc ttc ggc ctg ctc acc acc cga aac cgc ctc agc 835Ile Ala Leu Ser Val Phe Gly Leu Leu Thr Thr Arg Asn Arg Leu Ser230 235 240 245gca gcc gtg ctt gtg ggt acc gtt ggt gtg ggt gtt tcc ttc cag atg 883Ala Ala Val Leu Val Gly Thr Val Gly Val Gly Val Ser Phe Gln Met
250 255 260cta ctt ctg ggc gct ccc gat gtt gca ctt acc cag ttc ctg gta gaa 931Leu Leu Leu Gly Ala Pro Asp Val Ala Leu Thr Gln Phe Leu Val Glu
265 270 275ggc ctc gtc gtg gta atc atc atg atg gtt gtc cgg cac cag cct gcc 979Gly Leu Val Val Val Ile Ile Met Met Val Val Arg His Gln Pro Ala
280 285 290aac ttc aag cgc atc aag ccc agc aga agg cgc agc acc gtt ctt gtc 1027Asn Phe Lys Arg Ile Lys Pro Ser Arg Arg Arg Ser Thr Val Leu Val
295 300 305gcc gtc ctt gct gcc ttc gcc gca ttc atg gcg gtg tgg gga ttg ctt 1075Ala Val Leu Ala Ala Phe Ala Ala Phe Met Ala Val Trp Gly Leu Leu310 315 320 325ggc cgt cac gaa cgt tct gag ctg gcc atg tgg tac ctc aac caa ggt 1123Gly Arg His Glu Arg Ser Glu Leu Ala Met Trp Tyr Leu Asn Gln Gly
330 335 340cca gag atc acc tct ggc gcc aac gtg gtg aac acc atc ctc gtg gaa 1171Pro Glu Ile Thr Ser Gly Ala Asn Val Val Asn Thr Ile Leu Val Glu
345 350 355ttc cgt gca ctg gat acg ttg ggc gag ctc tcc gtg ctt ggc atg gca 1219Phe Arg Ala Leu Asp Thr Leu Gly Glu Leu Ser Val Leu Gly Met Ala
360 365 370gct gtc gtc atc ggt gcg atg gtg gct tcc atg cct cgt cat ccg ttt 1267Ala Val Val Ile Gly Ala Met Val Ala Ser Met Pro Arg His Pro Phe
375 380 385gcc aag ggc acc cac cct cgc ccc ttt ggc caa tca cag ttg aac tcc 1315Ala Lys Gly Thr His Pro Arg Pro Phe Gly Gln Ser Gln Leu Asn Ser390 395 400 405att ccg ctg cgc atg ctg ctt aag gtg ctg gtt cca gcg cta tgc ttc 1363Ile Pro Leu Arg Met Leu Leu Lys Val Leu Val Pro Ala Leu Cys Phe
410 415 420ttg agc ttc atg gtg ttc atg cgt gga cac aat gat ccg gga ggc ggt 1411Leu Ser Phe Met Val Phe Met Arg Gly His Asn Asp Pro Gly Gly Gly
425 430 435ttc atc gca gcc cta att gcc ggt ggc gcg ctg atg ctc ctg tac ctg 1459Phe Ile Ala Ala Leu Ile Ala Gly Gly Ala Leu Met Leu Leu Tyr Leu
440 445 450tcc aag gcc aaa gat ggc cgc att ttc cgc ccg aat gtt cct ttc att 1507Ser Lys Ala Lys Asp Gly Arg Ile Phe Arg Pro Asn Val Pro Phe Ile
455 460 465ctc act ggt gcg ggc atc ttg atg gca gtg ttc tcg ggc gta ctg gga 1555Leu Thr Gly Ala Gly Ile Leu Met Ala Val Phe Ser Gly Val Leu Gly470 475 480 485ctc acc cac ggt tct ttc ctg tac gcc atc cac ttc aac ttc gta ggc 1603Leu Thr His Gly Ser Phe Leu Tyr Ala Ile His Phe Asn Phe Val Gly
490 495 500cag cac tgg acc acc tcg atg atc ttc gac ctc ggc gtg tac ctg gcc 1651Gln His Trp Thr Thr Ser Met Ile Phe Asp Leu Gly Val Tyr Leu Ala
505 510 515gtg ttg ggc atg gtg tcc atg gca atc aac ggc ctg ggc gga tac ctg 1699Val Leu Gly Met Val Ser Met Ala Ile Asn Gly Leu Gly Gly Tyr Leu
520 525 530cgc cca ggt acc gac aat gca gat ctg gac tac gcc cgc cga agt ggc 1747Arg Pro Gly Thr Asp Asn Ala Asp Leu Asp Tyr Ala Arg Arg Ser Gly
535 540 545cca ctg cca gca acg cca acg gtt gaa ccc gaa cca gaa ggc gat gaa 1795Pro Leu Pro Ala Thr Pro Thr Val Glu Pro Glu Pro Glu Gly Asp Glu550 555 560 565gac tgg ccc gaa ccc arc aac ccc gca ggc gat aac aaa gag gag gca 1843Asp Trp Pro Glu Pro Ile Asn Pro Ala Gly Asp Asn Lys Glu Glu Ala
570 575 580aac cga tgattctcgc actgacagtc gcg 1872Asn Arg<210>686<211>583<212>PRT<213>谷氨酸棒杆菌<400>686Met Ala Ser Val Pro Pro Leu Leu Gly Phe Val Ser Lys Glu Gly Met1 5 10 15Ile Thr Ala Phe Met Asp Ala Pro Ile Gly Asn Ser Tyr Val Val Leu
20 25 30Leu Leu Val Gly Ala Ala Ile Gly Ala Val Leu Thr Phe Thr Tyr Ser
35 40 45Ala Lys Leu Val Leu Gly Ala Phe Val Asp Gly Pro Arg Asp Met Ser
50 55 60His Val Lys Glu Ala Pro Val Ser Leu Trp Leu Pro Ala Ala Leu Pro65 70 75 80Gly Leu Met Ser Leu Pro Leu Val Leu Val Leu Ser Leu Phe Asp Ala
85 90 95Pro Val Ser Ala Ala Ala Thr Ser Ala Ala Gly Glu Ala Ala His Met
100 105 110His Leu Ala Leu Trp His Gly Ile Asn Thr Pro Leu Leu Ile Ser Leu
115 120 125Gly Val Leu Val Ala Gly Ile Leu Gly Val Leu Phe Arg Lys Glu Leu
130 135 140Trp Lys Ile Ala Glu Thr Ser Pro Phe Pro Ile Ala Thr Gly Asn Asp145 150 155 160Ile Leu Ser Met Leu Val Tyr Arg Ala Asn Leu Leu Gly Lys Phe Phe
165 170 175Gly Arg Met Ala Asp Ser Met Ser Pro Arg Arg His Leu Val Ser Leu
180 185 190Ile Val Leu Leu Trp Ala Leu Ala Ala Phe Ala Thr Ile His Pro Ser
195 200 205Val Gln Leu Ala Pro Lys Gln Pro Gly Ile Asp Arg Trp Ile Asp Leu
210 215 220Ile Pro Leu Ala Ile Ile Ala Leu Ser Val Phe Gly Leu Leu Thr Thr225 230 235 240Arg Asn Arg Leu Ser Ala Ala Val Leu Val Gly Thr Val Gly Val Gly
245 250 255Val Ser Phe Gln Met Leu Leu Leu Gly Ala Pro Asp Val Ala Leu Thr
260 265 270Gln Phe Leu Val Glu Gly Leu Val Val Val Ile Ile Met Met Val Val
275 280 285Arg His Gln Pro Ala Asn Phe Lys Arg Ile Lys Pro Ser Arg Arg Arg
290 295 300Ser Thr Val Leu Val Ala Val Leu Ala Ala Phe Ala Ala Phe Met Ala305 310 315 320Val Trp Gly Leu Leu Gly Arg His Glu Arg Ser Glu Leu Ala Met Trp
325 330 335Tyr Leu Asn Gln Gly Pro Glu Ile Thr Ser Gly Ala Asn Val Val Asn
340 345 350Thr Ile Leu Val Glu Phe Arg Ala Leu Asp Thr Leu Gly Glu Leu Ser
355 360 365Val Leu Gly Met Ala Ala Val Val Ile Gly Ala Met Val Ala Ser Met
370 375 380Pro Arg His Pro Phe Ala Lys Gly Thr His Pro Arg Pro Phe Gly Gln385 390 395 400Ser Gln Leu Asn Ser Ile Pro Leu Arg Met Leu Leu Lys Val Leu Val
405 410 415Pro Ala Leu Cys Phe Leu Ser Phe Met Val Phe Met Arg Gly His Asn
420 425 430Asp Pro Gly Gly Gly Phe Ile Ala Ala Leu Ile Ala Gly Ala Leu
435 440 445Met Leu Leu Tyr Leu Ser Lys Ala Lys Asp Gly Arg Ile Phe Arg Pro
450 455 460Asn Val Pro Phe Ile Leu Thr Gly Ala Gly Ile Leu Met Ala Val Phe465 470 475 480Ser Gly Val Leu Gly Leu Thr His Gly Ser Phe Leu Tyr Ala Ile His
485 490 495Phe Asn Phe Val Gly Gln His Trp Thr Thr Ser Met Ile Phe Asp Leu
500 505 510Gly Val Tyr Leu Ala Val Leu Gly Met Val Ser Met Ala Ile Asn Gly
515 520 525Leu Gly Gly Tyr Leu Arg Pro Gly Thr Asp Asn Ala Asp Leu Asp Tyr
530 535 540Ala Arg Arg Ser Gly Pro Leu Pro Ala Thr Pro Thr Val Glu Pro Glu545 550 555 560Pro Glu Gly Asp Glu Asp Trp Pro Glu Pro Ile Asn Pro Ala Gly Asp
565 570 575Asn Lys Glu Glu Ala Asn Arg
580<210>687<211>1653<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1630)<223>RXN00595<400>687cgacgacacc cggtccatcg aaccagatga cgatcaatcg cctttgacta ctagcgctcg 60ttcagtcacc aacccaacag atcaggagga taaagcttaa atg gcc atg gat gtt 115
Met Ala Met Asp Val
1 5ctc ctt cct att ttc gtt gca gtt ccc ctt gct gcc tct gcc att gcg 163Leu Leu Pro Ile Phe Val Ala Val Pro Leu Ala Ala Ser Ala Ile Ala
10 15 20gtg ctt ctg ccg tgg cgt ctc atc cgc gat att ttg cac atc atc gtg 211Val Leu Leu Pro Trp Arg Leu Ile Arg Asp Ile Leu His Ile Ile Val
25 30 35cct ttc gcg ggt att ttt gct ggc atc tgg ttg ttt gca cac acc gct 259Pro Phe Ala Gly Ile Phe Ala Gly Ile Trp Leu Phe Ala His Thr Ala
40 45 50gaa cac ggc ccg att gct cac aac gtg ggc ctt tat gtc ggt ggc gtg 307Glu His Gly Pro Ile Ala His Asn Val Gly Leu Tyr Val Gly Gly Val
55 60 65gca atc ccc ttt gct gcc gat acg ttc agc gcc atc atg ttg atc acc 355Ala Ile Pro Phe Ala Ala Asp Thr Phe Ser Ala Ile Met Leu Ile Thr70 75 80 85acc tcg atc gtt gcg gtg gct gcc aac tgg ttt gcc acc atc gtc ggt 403Thr Ser Ile Val Ala Val Ala Ala Asn Trp Phe Ala Thr Ile Val Gly
90 95 100gaa acc cgc gcg cgt ttc tat cca gcg ctc aca ttg atg ctg atc acg 451Glu Thr Arg Ala Arg Phe Tyr Pro Ala Leu Thr Leu Met Leu Ile Thr
105 110 115ggc gtc aac ggt gct ctg ctg act gcc gat ctg ttc aac ttc ttt gtg 499Gly Val Asn Gly Ala Leu Leu Thr Ala Asp Leu Phe Asn Phe Phe Val
120 125 130ttc atc gaa gtg atg ctg ctg cct tcc tat ggt ttg atc gcc atg acc 547Phe Ile Glu Val Met Leu Leu Pro Ser Tyr Gly Leu Ile Ala Met Thr
135 140 145gga acg tgg gcg cgc cta gcc tct gga cga atc ttc gta cta gtc aat 595Gly Thr Trp Ala Arg Leu Ala Ser Gly Arg Ile Phe Val Leu Val Asn150 155 160 165ctc tct gcc tcc aca ttg ctg gtt gca ggt gtg gga atc gtc tac ggt 643Leu Ser Ala Ser Thr Leu Leu Val Ala Gly Val Gly Ile Val Tyr Gly
170 175 180gtc ata ggc tca gtc aac atc gca gct ctg caa gat gtc gta gag ggc 691Val Ile Gly Ser Val Asn Ile Ala Ala Leu Gln Asp Val Val Glu Gly
185 190 195aac ccc ctg gtt gcc agc gca atg ggc atc gtg gtt att gcc atc gcg 739Asn Pro Leu Val Ala Ser Ala Met Gly Ile Val Val Ile Ala Ile Ala
200 205 210gtt aaa gcc ggt gta ttc cca gtg cac aca tgg ctg cca cgc acc tat 787Val Lys Ala Gly Val Phe Pro Val His Thr Trp Leu Pro Arg Thr Tyr
215 220 225cct ggt aca tca gca gct gtg atg ggg ttg ttc tcc ggt ttg cac acc 835Pro Gly Thr Ser Ala Ala Val Met Gly Leu Phe Ser Gly Leu His Thr230 235 240 245aaa gtc gcg gta tac atg ctc tat cgc att tgg gtc cac att ttt aac 883Lys Val Ala Val Tyr Met Leu Tyr Arg Ile Trp Val His Ile Phe Asn
250 255 260atg gat ccc acg tgg aat tgg ctg att gtc gca ttc atg gtg ata tcc 931Met Asp Pro Thr Trp Asn Trp Leu Ile Val Ala Phe Met Val Ile Ser
265 270 275atg ctg gtc ggt ggc ttc gct gga ctt gct gaa aac tcc atc cgt cgc 979Met Leu Val Gly Gly Phe Ala Gly Leu Ala Glu Asn Ser Ile Arg Arg
280 285 290gtc ctt gcc tac caa atg gtc aac ggc atg cca ttt att ctc atc atg 1027Val Leu Ala Tyr Gln Met Val Asn Gly Met Pro Phe Ile Leu Ile Met
295 300 305atg gcg ttt acc tct gac gat cca cag cgc gca ctt gcc gct ggt ctg 1075Met Ala Phe Thr Ser Asp Asp Pro Gln Arg Ala Leu Ala Ala Gly Leu310 315 320 325ttg tac acc ctg cac cac atg atc acc atc gcc gca ttg gtg ctc act 1123Leu Tyr Thr Leu His His Met Ile Thr Ile Ala Ala Leu Val Leu Thr
330 335 340tcc ggc gca atc gaa gaa acc tac ggc acc ggt atg ttg tcc aag ctg 1171Ser Gly Ala Ile Glu Glu Thr Tyr Gly Thr Gly Met Leu Ser Lys Leu
345 350 355tct ggc ctt gca cgc cgc gaa ccc gtc gtc gca gca gtg ttc gct gca 1219Ser Gly Leu Ala Arg Arg Glu Pro Val Val Ala Ala Val Phe Ala Ala
360 365 370ggt gcc ttc tct gtt gtc ggt ttc cca ccg ttt tcc ggt atg tgg ggc 1267Gly Ala Phe Ser Val Val Gly Phe Pro Pro Phe Ser Gly Met Trp Gly
375 380 385aaa gcg ctc atc ctg ctc gag atc gcc cgc gtc ggc aat att gca gca 1315Lys Ala Leu Ile Leu Leu Glu Ile Ala Arg Val Gly Asn Ile Ala Ala390 395 400 405tgg atc gca atc gcc gcc atc atc atc gcc agc ctg ggc gca ctg ctc 1363Trp Ile Ala Ile Ala Ala Ile Ile Ile Ala Ser Leu Gly Ala Leu Leu
410 415 420tcg atg atc cgc gtg tgg cgt gaa gtc ttc tgg ggt ggc gca atg cac 1411Ser Met Ile Arg Val Trp Arg Glu Val Phe Trp Gly Gly Ala Met His
425 430 435cag cgc ggc gtc tcg ccg cag ctg cgc atc agc cca gca aaa atc gcc 1459Gln Arg Gly Val Ser Pro Gln Leu Arg Ile Ser Pro Ala Lys Ile Ala
440 445 450cca gcg ctc agc ctg atc att tta tcg gta ggc atg ttc atc ttc gcg 1507Pro Ala Leu Ser Leu Ile Ile Leu Ser Val Gly Met Phe Ile Phe Ala
455 460 465ggc ccg ctt atc gac gcg acc ctc acc gcc acc gac ggc ctc ttg aac 1555Gly Pro Leu Ile Asp Ala Thr Leu Thr Ala Thr Asp Gly Leu Leu Asn470 475 480 485acc gat gca tac caa cag gct gtg ctc ggt gaa aat gcc atc gga gtg 1603Thr Asp Ala Tyr Gln Gln Ala Val Leu Gly Glu Asn Ala Ile Gly Val
490 495 500cca agc cct agc tac cag gga gga aac taatgcttaa cgccctgaaa 1650Pro Ser Pro Ser Tyr Gln Gly Gly Asn
505 510ttc 1653<210>688<211>510<212>PRT<213>谷氨酸棒杆菌<400>688Met Ala Met Asp Val Leu Leu Pro Ile Phe Val Ala Val Pro Leu Ala1 5 10 15Ala Ser Ala Ile Ala Val Leu Leu Pro Trp Arg Leu Ile Arg Asp Ile
20 25 30Leu His Ile Ile Val Pro Phe Ala Gly Ile Phe Ala Gly Ile Trp Leu
35 40 45Phe Ala His Thr Ala Glu His Gly Pro Ile Ala His Asn Val Gly Leu
50 55 60Tyr Val Gly Gly Val Ala Ile Pro Phe Ala Ala Asp Thr Phe Ser Ala65 70 75 80Ile Met Leu Ile Thr Thr Ser Ile Val Ala Val Ala Ala Asn Trp Phe
85 90 95Ala Thr Ile Val Gly Glu Thr Arg Ala Arg Phe Tyr Pro Ala Leu Thr
100 105 110Leu Met Leu Ile Thr Gly Val Asn Gly Ala Leu Leu Thr Ala Asp Leu
115 120 125Phe Asn Phe Phe Val Phe Ile Glu Val Met Leu Leu Pro Ser Tyr Gly
130 135 140Leu Ile Ala Met Thr Gly Thr Trp Ala Arg Leu Ala Ser Gly Arg Ile145 150 155 160Phe Val Leu Val Asn Leu Ser Ala Ser Thr Leu Leu Val Ala Gly Val
165 170 175Gly Ile Val Tyr Gly Val Ile Gly Ser Val Asn Ile Ala Ala Leu Gln
180 185 190Asp Val Val Glu Gly Asn Pro Leu Val Ala Ser Ala Met Gly Ile Val
195 200 205Val Ile Ala Ile Ala Val Lys Ala Gly Val Phe Pro Val His Thr Trp
210 215 220Leu Pro Arg Thr Tyr Pro Gly Thr Ser Ala Ala Val Met Gly Leu Phe225 230 235 240Ser Gly Leu His Thr Lys Val Ala Val Tyr Met Leu Tyr Arg Ile Trp
245 250 255Val His Ile Phe Asn Met Asp Pro Thr Trp Asn Trp Leu Ile Val Ala
260 265 270Phe Met Val Ile Ser Met Leu Val Gly Gly Phe Ala Gly Leu Ala Glu
275 280 285Asn Ser Ile Arg Arg Val Leu Ala Tyr Gln Met Val Asn Gly Met Pro
290 295 300Phe Ile Leu Ile Met Met Ala Phe Thr Ser Asp Asp Pro Gln Arg Ala305 310 315 320Leu Ala Ala Gly Leu Leu Tyr Thr Leu His His Met Ile Thr Ile Ala
325 330 335Ala Leu Val Leu Thr Ser Gly Ala Ile Glu Glu Thr Tyr Gly Thr Gly
340 345 350Met Leu Ser Lys Leu Ser Gly Leu Ala Arg Arg Glu Pro Val Val Ala
355 360 365Ala Val Phe Ala Ala Gly Ala Phe Ser Val Val Gly Phe Pro Pro Phe
370 375 380Ser Gly Met Trp Gly Lys Ala Leu Ile Leu Leu Glu Ile Ala Arg Val385 390 395 400Gly Asn Ile Ala Ala Trp Ile Ala Ile Ala Ala Ile Ile Ile Ala Ser
405 410 415Leu Gly Ala Leu Leu Ser Met Ile Arg Val Trp Arg Glu Val Phe Trp
420 425 430Gly Gly Ala Met His Gln Arg Gly Val Ser Pro Gln Leu Arg Ile Ser
435 440 445Pro Ala Lys Ile Ala Pro Ala Leu Ser Leu Ile Ile Leu Ser Val Gly
450 455 460Met Phe Ile Phe Ala Gly Pro Leu Ile Asp Ala Thr Leu Thr Ala Thr465 470 475 480Asp Gly Leu Leu Asn Thr Asp Ala Tyr Gln Gln Ala Val Leu Gly Glu
485 490 495Asn Ala Ile Gly Val Pro Ser Pro Ser Tyr Gln Gly Gly Asn
500 505 510<210>689<211>865<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(865)<223>FRXA00608<400>689cgacgacacc cggtccatcg aaccagatga cgatcaatcg cctttgacta ctagcgctcg 60ttcagtcacc aacccaacag atcaggagga taaagcttaa atg gcc atg gat gtt 115
Met Ala Met Asp Val
1 5ctc ctt cct att ttc gtt gca gtt ccc ctt gct gcc tct gcc att gcg 163Leu Leu Pro Ile Phe Val Ala Val Pro Leu Ala Ala Ser Ala Ile Ala
10 15 20gtg ctt ctg ccg tgg cgt ctc atc cgc gat att ttg cac atc atc gtg 211Val Leu Leu Pro Trp Arg Leu Ile Arg Asp Ile Leu His Ile Ile Val
25 30 35cct ttc gcg ggt att ttt gct ggc atc tgg ttg ttt gca cac acc gct 259Pro Phe Ala Gly Ile Phe Ala Gly Ile Trp Leu Phe Ala His Thr Ala
40 45 50gaa cac ggc ccg att gct cac aac gtg ggc ctt tat gtc ggt ggc gtg 307Glu His Gly Pro Ile Ala His Asn Val Gly Leu Tyr Val Gly Gly Val
55 60 65gca atc ccc ttt gct gcc gat acg ttc agc gcc atc atg ttg atc acc 355Ala Ile Pro Phe Ala Ala Asp Thr Phe Ser Ala Ile Met Leu Ile Thr70 75 80 85acc tcg atc gtt gcg gtg gct gcc aac tgg ttt gcc acc atc gtc ggt 403Thr Ser Ile Val Ala Val Ala Ala Asn Trp Phe Ala Thr Ile Val Gly
90 95 100gaa acc cgc gcg cgt ttc tat cca gcg ctc aca ttg atg ctg atc acg 451Glu Thr Arg Ala Arg Phe Tyr Pro Ala Leu Thr Leu Met Leu Ile Thr
105 110 115ggc gtc aac ggt gct ctg ctg act gcc gat ctg ttc aac ttc ttt gtg 499Gly Val Asn Gly Ala Leu Leu Thr Ala Asp Leu Phe Asn Phe Phe Val
120 125 130ttc atc gaa gtg atg ctg ctg cct tcc tat ggt ttg atc gcc atg aac 547Phe Ile Glu Val Met Leu Leu Pro Ser Tyr Gly Leu Ile Ala Met Thr
135 140 145gga acg tgg gcg cgc cta gcc tct gga cga atc ttc gta cta gtc aat 595Gly Thr Trp Ala Arg Leu Ala Ser Gly Arg Ile Phe Val Leu Val Asn150 155 160 165ctc tct gcc tcc aca ttg ctg gtt gca ggt gtg gga atc gtc tac ggt 643Leu Ser Ala Ser Thr Leu Leu Val Ala Gly Val Gly Ile Val Tyr Gly
170 175 180gtc ata ggc tca gtc aac atc gca gct ctg caa gat gtc gta gag ggc 691Val Ile Gly Ser Val Asn Ile Ala Ala Leu Gln Asp Val Val Glu Gly
185 190 195aac ccc ctg gtt gcc agc gca atg ggc atc gtg gtt att gcc atc gcg 739Asn Pro Leu Val Ala Ser Ala Met Gly Ile Val Val Ile Ala Ile Ala
200 205 210gtt aaa gcc ggt gta ttc cca gtg cac aca tgg ctg cca cgc acc tat 787Val Lys Ala Gly Val Phe Pro Val His Thr Trp Leu Pro Arg Thr Tyr
215 220 225cct ggt aca tca gca gct gtg atg ggg ttg ttc tcc ggt ttg cac acc 835Pro Gly Thr Ser Ala Ala Val Met Gly Leu Phe Ser Gly Leu His Thr230 235 240 245aaa gtc gcg gta tac atg ctc tat cgc att 865Lys Val Ala Val Tyr Met Leu Tyr Arg Ile
250 255<210>690<211>255<212>PRT<213>谷氨酸棒杆菌<400>690Met Ala Met Asp Val Leu Leu Pro Ile Phe Val Ala Val Pro Leu Ala1 5 10 15Ala Ser Ala Ile Ala Val Leu Leu Pro Trp Arg Leu Ile Arg Asp Ile
20 25 30Leu His Ile Ile Val Pro Phe Ala Gly Ile Phe Ala Gly Ile Trp Leu
35 40 45Phe Ala His Thr Ala Glu His Gly Pro Ile Ala His Asn Val Gly Leu
50 55 60Tyr Val Gly Gly Val Ala Ile Pro Phe Ala Ala Asp Thr Phe Ser Ala65 70 75 80Ile Met Leu Ile Thr Thr Ser Ile Val Ala Val Ala Ala Asn Trp Phe
85 90 95Ala Thr Ile Val Gly Glu Thr Arg Ala Arg Phe Tyr Pro Ala Leu Thr
100 105 110Leu Met Leu Ile Thr Gly Val Asn Gly Ala Leu Leu Thr Ala Asp Leu
115 120 125Phe Asn Phe Phe Val Phe Ile Glu Val Met Leu Leu Pro Ser Tyr Gly
130 135 140Leu Ile Ala Met Thr Gly Thr Trp Ala Arg Leu Ala Ser Gly Arg Ile145 150 155 160Phe Val Leu Val Asn Leu Ser Ala Ser Thr Leu Leu Val Ala Gly Val
165 170 175Gly Ile Val Tyr Gly Val Ile Gly Ser Val Asn Ile Ala Ala Leu Gln
180 185 190Asp Val Val Glu Gly Asn Pro Leu Val Ala Ser Ala Met Gly Ile Val
195 200 205Val Ile Ala Ile Ala Val Lys Ala Gly Val Phe Pro Val His Thr Trp
210 215 220Leu Pro Arg Thr Tyr Pro Gly Thr Ser Ala Ala Val Met Gly Leu Phe225 230 235 240Ser Gly Leu His Thr Lys Val Ala Val Tyr Met Leu Tyr Arg Ile
245 250 255<210>691<211>2118<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(2118)<223>RXA00913<400>691att att tcc gtg gtg ggc att ggt acc cgc gaa gct ttg ctg gca ggt 48Ile Ile Ser Val Val Gly Ile Gly Thr Arg Glu Ala Leu Leu Ala Gly1 5 10 15ctt gca ctg acc gtt gcg cac tcc ttg ttt aag gca aca ttg ttc atg 96Leu Ala Leu Thr Val Ala His Ser Leu Phe Lys Ala Thr Leu Phe Met
20 25 30aca gtt ggt gcc att gac cac acc acc gga act cgt gat att cgt aaa 144Thr Val Gly Ala Ile Asp His Thr Thr Gly Thr Arg Asp Ile Arg Lys
35 40 45ctc tcc ggt ctg tgg cgt aaa caa ccg atc ctg ttc gcc gtt gct gct 192Leu Ser Gly Leu Trp Arg Lys Gln Pro Ile Leu Phe Ala Val Ala Ala
50 55 60gtt tcg gcg gcg tcc atg gct ggt att ccg cca ctg ttt ggt ttt atc 240Val Ser Ala Ala Ser Met Ala Gly Ile Pro Pro Leu Phe Gly Phe Ile65 70 75 80gcc aag gaa aca gcg ctg gat acc gtg ttg aat gag cag atg ttg cat 288Ala Lys Glu Thr Ala Leu Asp Thr Val Leu Asn Glu Gln Met Leu His
85 90 95ggc atg cca ggt cga ttg atg ctg gct ggc atc gtt ttg ggt tcc atc 336Gly Met Pro Gly Arg Leu Met Leu Ala Gly Ile Val Leu Gly Ser Ile
100 105 110ttc acc atg gca tat tcc tgc tac ttc ctg tac gaa gcc ttt gcc acg 384Phe Thr Met Ala Tyr Ser Cys Tyr Phe Leu Tyr Glu Ala Phe Ala Thr
115 120 125aag cac tcc aaa ttc cca gag gcc aac ggt gtc tca cct gca gtg gag 432Lys His Ser Lys Phe Pro Glu Ala Asn Gly Val Ser Pro Ala Val Glu
130 135 140gca atg cat ccg gtg aag ttt aag ctg tgg atc gca cct gtc atc ctg 480Ala Met His Pro Val Lys Phe Lys Leu Trp Ile Ala Pro Val Ile Leu145 150 155 160gct att ttg acc gta gtg ttt ggt gtt ttc ccc aag cca gtg tcg gaa 528Ala Ile Leu Thr Val Val Phe Gly Val Phe Pro Lys Pro Val Ser Glu
165 170 175gca att gtc acg cat ctt gat aac gtc acg cca tcg ctt gat gat gtc 576Ala Ile Val Thr His Leu Asp Asn Val Thr Pro Ser Leu Asp Asp Val
180 185 190cac acc aaa ctg gcc ttg tgg cat ggt ctg aat cta ccg ctg ctg ctg 624His Thr Lys Leu Ala Leu Trp His Gly Leu Asn Leu Pro Leu Leu Leu
195 200 205tct gtg gtg atc atc att tcc gga ttc atc atc ttc tgg gag cga gac 672Ser Val Val Ile Ile Ile Ser Gly Phe Ile Ile Phe Trp Glu Arg Asp
210 215 220acc gtc gaa cgt ttg cgc cct aac acc gca gcg ttt ggc agt gcc gat 720Thr Val Glu Arg Leu Arg Pro Asn Thr Ala Ala Phe Gly Ser Ala Asp225 230 235 240acc gcc tac gac gcc att ctt gat gca ctg cgt gtg ctc tcc cac cgc 768Thr Ala Tyr Asp Ala Ile Leu Asp Ala Leu Arg Val Leu Ser His Arg
245 250 255ctg act gca tcc acc cag cgt ggt tct ttg acc ctg aac gtc ggt gtg 816Leu Thr Ala Ser Thr Gln Arg Gly Ser Leu Thr Leu Asn Val Gly Val
260 265 270atc ttc ttc gtc ctc acg att gtt ccg ctg atc gct ttg atc act ggc 864Ile Phe Phe Val Leu Thr Ile Val Pro Leu Ile Ala Leu Ile Thr Gly
275 280 285gaa caa agc gat gtc cgc atg gag ctg tgg gat agc cct att cag ggc 912Glu Gln Ser Asp Val Arg Met Glu Leu Trp Asp Ser Pro Ile Gln Gly
290 295 300ttc atc gcg gcc atc att atc gtc gtt gcg att gtg gca acc acc atg 960Phe Ile Ala Ala Ile Ile Ile Val Val Ala Ile Val Ala Thr Thr Met305 310 315 320gat aac cgt ttg tct gcg ctg att ttg gtg ggt gtg aca ggt tat ggc 1008Asp Asn Arg Leu Ser Ala Leu Ile Leu Val Gly Val Thr Gly Tyr Gly
325 330 335att gcc gtt atc ttc gcg cta cat ggc gca ccg gac ttg gcg cta acc 1056Ile Ala Val Ile Phe Ala Leu His Gly Ala Pro Asp Leu Ala Leu Thr
340 345 350cag gtg ctg gtg gag acc atc gtc atg gtg gta ttc atg ctg gtg ctg 1104Gln Val Leu Val Glu Thr Ile Val Met Val Val Phe Met Leu Val Leu
355 360 365cgt aaa atg ccg aca gaa gtt gcg tgg aag gca gaa cct aaa cag tct 1152Arg Lys Met Pro Thr Glu Val Ala Trp Lys Ala Glu Pro Lys Gln Ser
370 375 380cgc gtg cga gcg tgg ctt gct ggc gcc acc gga ttg tcc gtt gtt att 1200Arg Val Arg Ala Trp Leu Ala Gly Ala Thr Gly Leu Ser Val Val Ile385 390 395 400gtc acc att ttt gcc atg aat gct cgc acc act gaa ccg atc tct gta 1248Val Thr Ile Phe Ala Met Asn Ala Arg Thr Thr Glu Pro Ile Ser Val
405 410 415tac atg cag gat ctg gcc tat gag atc gga cat ggc gca aac acc gtc 1296Tyr Met Gln Asp Leu Ala Tyr Glu Ile Gly His Gly Ala Asn Thr Val
420 425 430aac gta ctg ctc gta gac ctg cgt ggt ttt gat acc ttc ggt gaa att 1344Asn Val Leu Leu Val Asp Leu Arg Gly Phe Asp Thr Phe Gly Glu Ile
435 440 445tcc gtc ctt gtg atc gcg gca acc ggt atc gcc tcc ctg gtc tac cga 1392Ser Val Leu Val Ile Ala Ala Thr Gly Ile Ala Ser Leu Val Tyr Arg
450 455 460aac cgc agc ttc cgc aag gat tct cgc aga cca acc ctg gct acc act 1440Asn Arg Ser Phe Arg Lys Asp Ser Arg Arg Pro Thr Leu Ala Thr Thr465 470 475 480ggt cgc cgt tgg ttg gct gct gct gtt gat acc gaa agg gcg cag aac 1488Gly Arg Arg Trp Leu Ala Ala Ala Val Asp Thr Glu Arg Ala Gln Asn
485 490 495cgc tcg ctg atg gtt gat gtg gca acg cgc atc ctc ttc cct gcc atg 1536Arg Ser Leu Met Val Asp Val Ala Thr Arg Ile Leu Phe Pro Ala Met
500 505 510atc atg ttg tct gtg tac ttc ttc ttc gcc gga cac aac gcg ccg ggc 1584Ile Met Leu Ser Val Tyr Phe Phe Phe Ala Gly His Asn Ala Pro Gly
515 520 525ggc gga ttc gcc ggc ggc ctt gtt gcc tcc ttg gcg ttc gcc ttg cgc 1632Gly Gly Phe Ala Gly Gly Leu Val Ala Ser Leu Ala Phe Ala Leu Arg
530 535 540tac ctt gcc ggt gga cgt gaa gaa ctt gaa gaa gcg ttg cct atc gac 1680Tyr Leu Ala Gly Gly Arg Glu Glu Leu Glu Glu Ala Leu Pro Ile Asp545 550 555 560gcc ggc cgt atc ttg gga act gga cta ttt gtt tct gca act gca gtg 1728Ala Gly Arg Ile Leu Gly Thr Gly Leu Phe Val Ser Ala Thr Ala Val
565 570 575ctg tgg ccc atg gtt ctt ctt ggt gaa cca ccg ctg acc tcc cat att 1776Leu Trp Pro Met Val Leu Leu Gly Glu Pro Pro Leu Thr Ser His Ile
580 585 590tgg gat ctc aca ctg cca ctt atc ggt gag att cac att gca tcc gcg 1824Trp Asp Leu Thr Leu Pro Leu Ile Gly Glu Ile His Ile Ala Ser Ala
595 600 605ctg ctc ttt gac ctt ggt gtc tac ctg atc gtc atc ggt ttg acc atg 1872Leu Leu Phe Asp Leu Gly Val Tyr Leu Ile Val Ile Gly Leu Thr Met
610 615 620cac att ctc aac agt ttg ggc ggc cag ctc gac cgc gat gag gaa atg 1920His Ile Leu Asn Ser Leu Gly Gly Gln Leu Asp Arg Asp Glu Glu Met625 630 635 640cgt aag cag cgt gcg cgc gac cga gct cga cgc ttg gcg cgc aac cag 1968Arg Lys Gln Arg Ala Arg Asp Arg Ala Arg Arg Leu Ala Arg Asn Gln
645 650 655cgt cga gaa gca gca acc gtc ggc gca cgc agg tcg aac gag aaa tcg 2016Arg Arg Glu Ala Ala Thr Val Gly Ala Arg Arg Ser Asn Glu Lys Ser
660 665 670aca cgc caa atg ccg acg att cgg cct cca ggg gca gac aca gaa tcg 2064Thr Arg Gln Met Pro Thr Ile Arg Pro Pro Gly Ala Asp Thr Glu Ser
675 680 685gtg gag cag aac ggt gag aac cag acg tcg ata agc aca aag cgt tta 2112Val Glu Gln Asn Gly Glu Asn Gln Thr Ser Ile Ser Thr Lys Arg Leu
690 695 700aag cag 2118Lys Gln705<210>692<211>706<212>PRT<213>谷氨酸棒杆菌<400>692Ile Ile Ser Val Val Gly Ile Gly Thr Arg Glu Ala Leu Leu Ala Gly1 5 10 15Leu Ala Leu Thr Val Ala His Ser Leu Phe Lys Ala Thr Leu Phe Met
20 25 30Thr Val Gly Ala Ile Asp His Thr Thr Gly Thr Arg Asp Ile Arg Lys
35 40 45Leu Ser Gly Leu Trp Arg Lys Gln Pro Ile Leu Phe Ala Val Ala Ala
50 55 60Val Ser Ala Ala Ser Met Ala Gly Ile Pro Pro Leu Phe Gly Phe Ile65 70 75 80Ala Lys Glu Thr Ala Leu Asp Thr Val Leu Asn Glu Gln Met Leu His
85 90 95Gly Met Pro Gly Arg Leu Met Leu Ala Gly Ile Val Leu Gly Ser Ile
100 105 110Phe Thr Met Ala Tyr Ser Cys Tyr Phe Leu Tyr Glu Ala Phe Ala Thr
115 120 125Lys His Ser Lys Phe Pro Glu Ala Asn Gly Val Ser Pro Ala Val Glu
130 135 140Ala Met His Pro Val Lys Phe Lys Leu Trp Ile Ala Pro Val Ile Leu145 150 155 160Ala Ile Leu Thr Val Val Phe Gly Val Phe Pro Lys Pro Val Ser Glu
165 170 175Ala Ile Val Thr His Leu Asp Asn Val Thr Pro Ser Leu Asp Asp Val
180 185 190His Thr Lys Leu Ala Leu Trp His Gly Leu Asn Leu Pro Leu Leu Leu
195 200 205Ser Val Val Ile Ile Ile Ser Gly Phe Ile Ile Phe Trp Glu Arg Asp
210 215 220Thr Val Glu Arg Leu Arg Pro Asn Thr Ala Ala Phe Gly Ser Ala Asp225 230 235 240Thr Ala Tyr Asp Ala Ile Leu Asp Ala Leu Arg Val Leu Ser His Arg
245 250 255Leu Thr Ala Ser Thr Gln Arg Gly Ser Leu Thr Leu Asn Val Gly Val
260 265 270Ile Phe Phe Val Leu Thr Ile Val Pro Leu Ile Ala Leu Ile Thr Gly
275 280 285Glu Gln Ser Asp Val Arg Met Glu Leu Trp Asp Ser Pro Ile Gln Gly
290 295 300Phe Ile Ala Ala Ile Ile Ile Val Val Ala Ile Val Ala Thr Thr Met305 310 315 320Asp Asn Arg Leu Ser Ala Leu Ile Leu Val Gly Val Thr Gly Tyr Gly
325 330 335Ile Ala Val Ile Phe Ala Leu His Gly Ala Pro Asp Leu Ala Leu Thr
340 345 350Gln Val Leu Val Glu Thr Ile Val Met Val Val Phe Met Leu Val Leu
355 360 365Arg Lys Met Pro Thr Glu Val Ala Trp Lys Ala Glu Pro Lys Gln Ser
370 375 380Arg Val Arg Ala Trp Leu Ala Gly Ala Thr Leu Sar Val Val Ile Ile385 390 395 400Val Thr Ile Phe Ala Met Asn Ala Arg Thr Thr Glu Pro Ile Ser Val
405 410 415Tyr Met Gln Asp Leu Ala Tyr Glu Ile Gly His Gly Ala Asn Thr Val
420 425 430Asn Val Leu Leu Val Asp Leu Arg Gly Phe Asp Thr Phe Gly Glu Ile
435 440 445Ser Val Leu Val Ile Ala Ala Thr Gly Ile Ala Ser Leu Val Tyr Arg
450 455 460Asn Arg Ser Phe Arg Lys Asp Ser Arg Arg Pro Thr Leu Ala Thr Thr465 470 475 480Gly Arg Arg Trp Leu Ala Ala Ala Val Thr Glu Arg Ala Gln Asn
485 490 495Arg Ser Leu Met Val Asp Val Ala Thr Arg Ile Leu Phe Pro Ala Met
500 505 510Ile Met Leu Ser Val Tyr Phe Phe Phe Ala Gly His Asn Ala Pro Gly
515 520 525Gly Gly Phe Ala Gly Gly Leu Val Ala Ser Leu Ala Phe Ala Leu Arg
530 535 540Tyr Leu Ala Gly Gly Arg Glu Glu Leu Glu Glu Ala Leu Pro Ile Asp545 550 555 560Ala Gly Arg Ile Leu Gly Thr Gly Leu Phe Val Ser Ala Thr Ala Val
565 570 575Leu Trp Pro Met Val Leu Leu Gly Glu Pro Pro Leu Thr Ser His Ile
580 585 590Trp Asp Leu Thr Leu Pro Leu Ile Gly Glu Ile His Ile Ala Ser Ala
595 600 605Leu Leu Phe Asp Leu Gly Val Tyr Leu Ile Val Ile Gly Leu Thr Met
610 615 620His Ile Leu Asn Ser Leu Gly Gly Gln Leu Asp Arg Asp Glu Glu Met625 630 635 640Arg Lys Gln Arg Ala Arg Asp Arg Ala Arg Arg Leu Ala Arg Asn Gln
645 650 655Arg Arg Glu Ala Ala Thr Val Gly Ala Arg Arg Ser Asn Glu Lys Ser
660 665 670Thr Arg Gln Met Pro Thr Ile Arg Pro Pro Gly Ala Asp Thr Glu Ser
675 680 685Val Glu Gln Asn Gly Glu Asn Gln Thr Ser Ile Ser Thr Lys Arg Leu
690 695 700Lys Gln705<210>693<211>955<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(955)<223>RXA00909<400>693tcgatgtgtg ttgctaactg ggggtggcac gcacgttggc gttgttgttt ggtgtggctc 60cagagtaatc cacaacgcgc aaaggggaac tggagaacac gtg ctc att ctt ttt 115
Val Leu Ile Leu Phe
1 5ctc gcg ctc act gca gcc gca gta gtc gcc ccc atc ctg atc cga act 163Leu Ala Leu Thr Ala Ala Ala Val Val Ala Pro Ile Leu Ile Arg Thr
10 15 20ctc ggt cga cca gct ttt ggt ctg ctg gcg ctt gta cct ggc att ggt 211Leu Gly Arg Pro Ala Phe Gly Leu Leu Ala Leu Val Pro Gly Ile Gly
25 30 35ttt ttc tgg gtg ctt tcg gag ttc atc aaa ggc act ttc aag gat gga 259Phe Phe Trp Val Leu Ser Glu Phe Ile Lys Gly Thr Phe Lys Asp Gly
40 45 50ggt gaa ctc ctc ctc cac tat gcc tgg atg cct tcg gct cac ctc aat 307Gly Glu Leu Leu Leu His Tyr Ala Trp Met Pro Ser Ala His Leu Asn
55 60 65atc gat ttc cgt atg gat tcc ctc gcg gcg ctg ttc tca ctc atc gtc 355Ile Asp Phe Arg Met Asp Ser Leu Ala Ala Leu Phe Ser Leu Ile Val70 75 80 85tta ggc gtg ggc gcc cta gtg ctg ctg tac tgc tgg gga tat ttt gat 403Leu Gly Val Gly Ala Leu Val Leu Leu Tyr Cys Trp Gly Tyr Phe Asp
90 95 100tcc aac gcg ggt cgc ctc agt gcc ttt ggt gct gaa ctg gtg gcc ttc 451Ser Asn Ala Gly Arg Leu Ser Ala Phe Gly Ala Glu Leu Val Ala Phe
105 110 115gcc atg gcg atg ttt ggt ctt gtc att tca gac aac atc ctg ctg atg 499Ala Met Ala Met Phe Gly Leu Val Ile Ser Asp Asn Ile Leu Leu Met
120 125 130tac gtc ttc tgg gaa atc acc tcc gtt tta tcc ttc ctc ctg gtt ggt 547Tyr Val Phe Trp Glu Ile Thr Ser Val Leu Ser Phe Leu Leu Val Gly
135 140 145tat tac ggc gaa cgc gca tct tca cgt cgc tct gca ggt caa gcc ttg 595Tyr Tyr Gly Glu Arg Ala Ser Ser Arg Arg Ser Ala Gly Gln Ala Leu150 155 160 165atg gtg acc acc ctg ggt gga ttg gcc atg ctg gtg ggc atc att ttg 643Met Val Thr Thr Leu Gly Gly Leu Ala Met Leu Val Gly Ile Ile Leu
170 175 180atg ggt acc caa act ggc gtg tgg cga ttc tct gag atc cct gcc tac 691Met Gly Thr Gln Thr Gly Val Trp Arg Phe Ser Glu Ile Pro Ala Tyr
185 190 195tca agc tcc tgg gca gat gtg ccg tat att tcc gct gct gct gcc ctt 739Ser Ser Ser Trp Ala Asp Val Pro Tyr Ile Ser Ala Ala Ala Ala Leu
200 205 210atc ttg gct ggc gca cta tcc aaa tcg gct atc gca cca acc cac ttc 787Ile Leu Ala Gly Ala Leu Ser Lys Ser Ala Ile Ala Pro Thr His Phe
215 220 225tgg ctt ccc ggc gcg atg gcc gca cca acg ccg gtg tct gct tac ctg 835Trp Leu Pro Gly Ala Met Ala Ala Pro Thr Pro Val Ser Ala Tyr Leu230 235 240 245cac tcc gca gcg atg gtg aag gcg ggt att tac ctt gtg gct cgc ctc 883His Ser Ala Ala Met Val Lys Ala Gly Ile Tyr Leu Val Ala Arg Leu
250 255 260tct cca gac ctc aac gta gtt ggt tcg tgg tac ctg atc atc atc ccg 931Ser Pro Asp Leu Asn Val Val Gly Ser Trp Tyr Leu Ile Ile Ile Pro
265 270 275ttg ggc atg ttg acc atg ctc atg 955Leu Gly Met Leu Thr Met Leu Met
280 285<210>694<211>285<212>PRT<213>谷氨酸棒杆菌<400>694Val Leu Ile Leu Phe Leu Ala Leu Thr Ala Ala Ala Val Val Ala Pro1 5 10 15Ile Leu Ile Arg Thr Leu Gly Arg Pro Ala Phe Gly Leu Leu Ala Leu
20 25 30Val Pro Gly Ile Gly Phe Phe Trp Val Leu Ser Glu Phe Ile Lys Gly
35 40 45Thr Phe Lys Asp Gly Gly Glu Leu Leu Leu His Tyr Ala Trp Met Pro
50 55 60Ser Ala His Leu Asn Ile Asp Phe Arg Met Asp Ser Leu Ala Ala Leu65 70 75 80Phe Ser Leu Ile Val Leu Gly Val Gly Ala Leu Val Leu Leu Tyr Cys
85 90 95Trp Gly Tyr Phe Asp Ser Asn Ala Gly Arg Leu Ser Ala Phe Gly Ala
100 105 110Glu Leu Val Ala Phe Ala Met Ala Met Phe Gly Leu Val Ile Ser Asp
115 120 125Asn Ile Leu Leu Met Tyr Val Phe Trp Glu Ile Thr Ser Val Leu Ser
130 135 140Phe Leu Leu Val Gly Tyr Tyr Gly Glu Arg Ala Ser Ser Arg Arg Ser145 150 155 160Ala Gly Gln Ala Leu Met Val Thr Thr Leu Gly Gly Leu Ala Met Leu
165 170 175Val Gly Ile Ile Leu Met Gly Thr Gln Thr Gly Val Trp Arg Phe Ser
180 185 190Glu Ile Pro Ala Tyr Ser Ser Ser Trp Ala Asp Val Pro Tyr Ile Ser
195 200 205Ala Ala Ala Ala Leu Ile Leu Ala Gly Ala Leu Ser Lys Ser Ala Ile
210 215 220Ala Pro Thr His Phe Trp Leu Pro Gly Ala Met Ala Ala Pro Thr Pro225 230 235 240Val Ser Ala Tyr Leu His Ser Ala Ala Met Val Lys Ala Gly Ile Tyr
245 250 255Leu Val Ala Arg Leu Ser Pro Asp Leu Asn Val Val Gly Ser Trp Tyr
260 265 270Leu Ile Ile Ile Pro Leu Gly Met Leu Thr Met Leu Met
275 280 285<210>695<211>927<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(904)<223>RXA00700<400>695acgccccaca agtcgcaaaa atcaccgccg gcatccaaga ggaatcacac tggctcacag 60tctcggccgt gaaagctgcg ctagggcatg gtgaaatctc atg atc aac gcc atc 115
Met Ile Asn Ala Ile
1 5aca ctc aag ccc aaa acc ttc ctc acc tta agc ttc ctt gcg gtt ttg 163Thr Leu Lys Pro Lys Thr Phe Leu Thr Leu Ser Phe Leu Ala Val Leu
10 15 20agc atc gtg att ttc ttc tgg ccg ctg atc gtc aac ccg gaa tcc ttc 211Ser Ile Val Ile Phe Phe Trp Pro Leu Ile Val Asn Pro Glu Ser Phe
25 30 35ctg tcc gac aaa gcc caa gcg ccc ctc tac atc gcg atc gtc att ccc 259Leu Ser Asp Lys Ala Gln Ala Pro Leu Tyr Ile Ala Ile Val Ile Pro
40 45 50ctc gtg ctg gcc gct gtc atc gcc gaa atc agt gaa aac gga ttc gac 307Leu Val Leu Ala Ala Val Ile Ala Glu Ile Ser Glu Asn Gly Phe Asp
55 60 65gtt aaa gcc gta gcc atg ctc ggc gtc ctc acc gcc atg gtt gcc gta 355Val Lys Ala Val Ala Met Leu Gly Val Leu Thr Ala Met Val Ala Val70 75 80 85gtc cga cca ttc ggt gcc ggc gca gca ggc ttt gaa gca gtc ttc ttt 403Val Arg Pro Phe Gly Ala Gly Ala Ala Gly Phe Glu Ala Val Phe Phe
90 95 100gtc ctc atc ctc ggc gga cga gcc ttc gga ccc ggc ttc gga ttc atc 451Val Leu Ile Leu Gly Gly Arg Ala Phe Gly Pro Gly Phe Gly Phe Ile
105 110 115ctc ggc aac acc gga ctg ttc gca tcc gcg ctg ctc acc gca gga atc 499Leu Gly Asn Thr Gly Leu Phe Ala Ser Ala Leu Leu Thr Ala Gly Ile
120 125 130gga ccg tgg ctc ccc tac caa atg ctc gca gcc gcc tgg gtc agc ttc 547Gly Pro Trp Leu Pro Tyr Gln Met Leu Ala Ala Ala Trp Val Ser Phe
135 140 145ggc gcc ggc cta ctc ccc caa gta cgc ggc aaa aag gaa atg ctc atc 595Gly Ala Gly Leu Leu Pro Gln Val Arg Gly Lys Lys Glu Met Leu Ile150 155 160 165atc gtc cta tac gcc atc gtc tct tca ctc ggc tac gga acc atg atg 643Ile Val Leu Tyr Ala Ile Val Ser Ser Leu Gly Tyr Gly Thr Met Met
170 175 180aac atg agc ttc tgg ccc tac gcc atc ggt gtc acc agc ggg ctt tcc 691Asn Met Ser Phe Trp Pro Tyr Ala Ile Gly Val Thr Ser Gly Leu Ser
185 190 195ttc aca ccc ggc gcg ccc gtc ctg gaa aac ctc cac acc ttc atg ctg 739Phe Thr Pro Gly Ala Pro Val Leu Glu Asn Leu His Thr Phe Met Leu
200 205 210ttc tgc ctc acc aca tcc atg ggt tgg gat ctc ggc cgc gcc ttc ttc 787Phe Cys Leu Thr Thr Ser Met Gly Trp Asp Leu Gly Arg Ala Phe Phe
215 220 225acc tca gtg cta tta ctg ctc aca gcc aaa ccc gtt tta ggt gct tta 835Thr Ser Val Leu Leu Leu Leu Thr Ala Lys Pro Val Leu Gly Ala Leu230 235 240 245cga cgc gcc agc cgc cgc gcc gct ttc ggc gtc gag cgt gac ttc ggg 883Arg Arg Ala Ser Arg Arg Ala Ala Phe Gly Val Glu Arg Asp Phe Gly
250 255 260gag gcc ggg gtg cct cgg gtc taaagatttt gttggcttgc ttc 927Glu Ala Gly Val Pro Arg Val
265<210>696<211>268
<212>PRT<213>谷氨酸棒杆菌<400>696Met Ile Asn Ala Ile Thr Leu Lys Pro Lys Thr Phe Leu Thr Leu Ser1 5 10 15Phe Leu Ala Val Leu Ser Ile Val Ile Phe Phe Trp Pro Leu Ile Val
20 25 30Asn Pro Glu Ser Phe Leu Ser Asp Lys Ala Gln Ala Pro Leu Tyr Ile
35 40 45Ala Ile Val Ile Pro Leu Val Leu Ala Ala Val Ile Ala Glu Ile Ser
50 55 60Glu Asn Gly Phe Asp Val Lys Ala Val Ala Met Leu Gly Val Leu Thr65 70 75 80Ala Met Val Ala Val Val Arg Pro Phe Gly Ala Gly Ala Ala Gly Phe
85 90 95Glu Ala Val Phe Phe Val Leu Ile Leu Gly Gly Arg Ala Phe Gly Pro
100 105 110Gly Phe Gly Phe Ile Leu Gly Asn Thr Gly Leu Phe Ala Ser Ala Leu
115 120 125Leu Thr Ala Gly Ile Gly Pro Trp Leu Pro Tyr Gln Met Leu Ala Ala
130 135 140Ala Trp Val Ser Phe Gly Ala Gly Leu Leu Pro Gln Val Arg Gly Lys145 150 155 160Lys Glu Met Leu Ile Ile Val Leu Tyr Ala Ile Val Ser Ser Leu Gly
165 170 175Tyr Gly Thr Met Met Asn Met Ser Phe Trp Pro Tyr Ala Ile Gly Val
180 185 190Thr Ser Gly Leu Ser Phe Thr Pro Gly Ala Pro Val Leu Glu Asn Leu
195 200 205His Thr Phe Met Leu Phe Cys Leu Thr Thr Ser Met Gly Trp Asp Leu
210 215 220Gly Arg Ala Phe Phe Thr Ser Val Leu Leu Leu Leu Thr Ala Lys Pro225 230 235 240Val Leu Gly Ala Leu Arg Arg Ala Ser Arg Arg Ala Ala Phe Gly Val
245 250 255Glu Arg Asp Phe Gly Glu Ala Gly Val Pro Arg Val
260 265<210>697<211>1587<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1564)<223>RXN00483<400>697agacccaaga gtaaaatccc aggatttgct tatacttgcg ctcatggata atcaacttcg 60tcccactttg cattatcaag ctcaaaaccc gcaccggcga gtg ctg gtc acc ggt 115
Val Leu Val Thr Gly
1 5gcg aca ggc tac att ggc ggc agg ttg att act gag tta ctt gct gcc 163Ala Thr Gly Tyr Ile Gly Gly Arg Leu Ile Thr Glu Leu Leu Ala Ala
10 15 20ggt ttc caa gtt cgg gcc acc tcg agg aaa aaa aca agt ctt cag cgc 211Gly Phe Gln Val Arg Ala Thr Ser Arg Lys Lys Thr Ser Leu Gln Arg
25 30 35ttt gac tgg tac gag gac gtc gag gca gtg gaa gcg gat ctg act gac 259Phe Asp Trp Tyr Glu Asp Val Glu Ala Val Glu Ala Asp Leu Thr Asp
40 45 50gcg act gag tta gat acg tta ttt aag gat gta gac gtt gtt tac tat 307Ala Thr Glu Leu Asp Thr Leu Phe Lys Asp Val Asp Val Val Tyr Tyr
55 60 65cta gtg cat tcc atg gga ggt aag aat gtt gat ttt gaa gag caa gag 355Leu Val His Ser Met Gly Gly Lys Asn Val Asp Phe Glu Glu Gln Glu70 75 80 85caa cgc act gct gaa aat gta att caa gct gct gat caa gcc ggg ata 403Gln Arg Thr Ala Glu Asn Val Ile Gln Ala Ala Asp Gln Ala Gly Ile
90 95 100aaa cag att gtc tac ctt tcc ggc tta cac ccg cgt aat cga aaa ata 451Lys Gln Ile Val Tyr Leu Ser Gly Leu His Pro Arg Asn Arg Lys Ile
105 110 115gaa gaa cta tct aag cac atg cgc tca cgg gaa aag gtc gcc cag att 499Glu Glu Leu Ser Lys His Met Arg Ser Arg Glu Lys Val Ala Gln Ile
120 125 130ttg ctg gca ggc cag aca cca gct tta att tta agg gct gcc aca att 547Leu Leu Ala Gly Gln Thr Pro Ala Leu Ile Leu Arg Ala Ala Thr Ile
135 140 145att ggt tcc ggc tct gca tca ttt gaa ata atc cgt cat ctc acg gag 595Ile Gly Ser Gly Ser Ala Ser Phe Glu Ile Ile Arg His Leu Thr Glu150 155 160 165cgt ttg cct aga atg ata gcg cct cag tgg att act aat cag att gag 643Arg Leu Pro Arg Met Ile Ala Pro Gln Trp Ile Thr Asn Gln Ile Glu
170 175 180cct tta gca ata cgg gat gtt ttg cat tac cta atc tcg gcg gct gat 691Pro Leu Ala Ile Arg Asp Val Leu His Tyr Leu Ile Ser Ala Ala Asp
185 190 195tta aag gat cca gtc aac cgc tcc tgc gat att ggg tgt gga aag tcg 739Leu Lys Asp Pro Val Asn Arg Ser Cys Asp Ile Gly Cys Gly Lys Ser
200 205 210tat gaa ttt gcg gat cta ttg cgt atc tat gcc gat gtt cgg gga ctg 787Tyr Glu Phe Ala Asp Leu Leu Arg Ile Tyr Ala Asp Val Arg Gly Leu
215 220 225aaa cgt cat gta aat tcc gta cct ctc aat ttg ccc atg gac aag cta 835Lys Arg His Val Asn Ser Val Pro Leu Asn Leu Pro Met Asp Lys Leu230 235 240 245tcc ggt ctt tgg att agt cta gtg aca cct gtt cca ttt caa ttg tct 883Ser Gly Leu Trp Ile Ser Leu Val Thr Pro Val Pro Phe Gln Leu Ser
250 255 260ttc cct tta gct caa tca atg gct gag gat gcc gtc act gaa gag cac 931Phe Pro Leu Ala Gln Ser Met Ala Glu Asp Ala Val Thr Glu Glu His
265 270 275agc att aaa gat att att tca gat cca ccc gat ggt ttt att gag tat 979Ser Ile Lys Asp Ile Ile Ser Asp Pro Pro Asp Gly Phe Ile Glu Tyr
280 285 290cgg gaa gca gtg gag ctg gca tta gct gca gaa ttt gat cgt gga gtt 1027Arg Glu Ala Val Glu Leu Ala Leu Ala Ala Glu Phe Asp Arg Gly Val
295 300 305cca acg tca tgg gat cga agc tgg act gta caa caa ccg tgg gct ggc 1075Pro Thr Ser Trp Asp Arg Ser Trp Thr Val Gln Gln Pro Trp Ala Gly310 315 320 325cag cct acc gat cca gag tgg gcg ggc aaa gct gta tat gaa gac gtc 1123Gln Pro Thr Asp Pro Glu Trp Ala Gly Lys Ala Val Tyr Glu Asp Val
330 335 340cgc aca gaa gat act gat ctc cga gca gcg cag gtc tgg ccg atc att 1171Arg Thr Glu Asp Thr Asp Leu Arg Ala Ala Gln Val Trp Pro Ile Ile
345 350 355gaa ggt ttg ggt ggc gtg aac ggc tgg tat tct gca cca ctg cta tgg 1219Glu Gly Leu Gly Gly Val Asn Gly Trp Tyr Ser Ala Pro Leu Leu Trp
360 365 370cga ttg cgg ggt atc gct gac aga ctc atc ggc ggt cca ggt ttg ggc 1267Arg Leu Arg Gly Ile Ala Asp Arg Leu Ile Gly Gly Pro Gly Leu Gly
375 380 385gga cgg cgg gat ccc cgt cat ttg aaa ctt ggg gat cgc att gat tgg 1315Gly Arg Arg Asp Pro Arg His Leu Lys Leu Gly Asp Arg Ile Asp Trp390 395 400 405tgg cgg gtt act gag atc gat cca cca cat aga tta gtg ctc acc gca 1363Trp Arg Val Thr Glu Ile Asp Pro Pro His Arg Leu Val Leu Thr Ala
410 415 420gag atg aaa gta gat ggt ggc gct tgg ctg atc ctg gaa gtt gcg gac 1411Glu Met Lys Val Asp Gly Gly Ala Trp Leu Ile Leu Glu Val Ala Asp
425 430 435aag gaa aat ggc gga tgt act tat acc cag cgc gca ata ttt gag ccg 1459Lys Glu Asn Gly Gly Cys Thr Tyr Thr Gln Arg Ala Ile Phe Glu Pro
440 445 450aag ggt ttg ccc ggt tat ctc tat tgg tgg gtt gtt tca ccg ttc cat 1507Lys Gly Leu Pro Gly Tyr Leu Tyr Trp Trp Val Val Ser Pro Phe His
455 460 465gcg att att ttt cct tat atg cgt tcg aat att tta aaa gct gcg cgt 1555Ala Ile Ile Phe Pro Tyr Met Arg Ser Asn Ile Leu Lys Ala Ala Arg470 475 480 485aaa ctc act taatcgcaga gtaggcgtct aaa 1587Lys Leu Thr<210>698<211>488<212>PRT<213>谷氨酸棒杆菌<400>698Val Leu Val Thr Gly Ala Thr Gly Tyr Ile Gly Gly Arg Leu Ile Thr1 5 10 15Glu Leu Leu Ala Ala Gly Phe Gln Val Arg Ala Thr Ser Arg Lys Lys
20 25 30Thr Ser Leu Gln Arg Phe Asp Trp Tyr Glu Asp Val Glu Ala Val Glu
35 40 45Ala Asp Leu Thr Asp Ala Thr Glu Leu Asp Thr Leu Phe Lys Asp Val
50 55 60Asp Val Val Tyr Tyr Leu Val His Ser Met Gly Gly Lys Asn Val Asp65 70 75 80Phe Glu Glu Gln Glu Gln Arg Thr Ala Glu Asn Val Ile Gln Ala Ala
85 90 95Asp Gln Ala Gly Ile Lys Gln Ile Val Tyr Leu Ser Gly Leu His Pro
100 105 110Arg Asn Arg Lys Ile Glu Glu Leu Ser Lys His Met Arg Ser Arg Glu
115 120 125Lys Val Ala Gln Ile Leu Leu Ala Gly Gln Thr Pro Ala Leu Ile Leu
130 135 140Arg Ala Ala Thr Ile Ile Gly Ser Gly Ser Ala Ser Phe Glu Ile Ile145 150 155 160Arg His Leu Thr Glu Arg Leu Pro Arg Met Ile Ala Pro Gln Trp Ile
165 170 175Thr Asn Gln Ile Glu Pro Leu Ala Ile Arg Asp Val Leu His Tyr Leu
180 185 190Ile Ser Ala Ala Asp Leu Lys Asp Pro Val Asn Arg Ser Cys Asp Ile
195 200 205Gly Cys Gly Lys Ser Tyr Glu Phe Ala Asp Leu Leu Arg Ile Tyr Ala
210 215 220Asp Val Arg Gly Leu Lys Arg His Val Asn Ser Val Pro Leu Asn Leu225 230 235 240Pro Met Asp Lys Leu Ser Gly Leu Trp Ile Ser Leu Val Thr Pro Val
245 250 255Pro Phe Gln Leu Ser Phe Pro Leu Ala Gln Ser Met Ala Glu Asp Ala
260 265 270Val Thr Glu Glu His Ser Ile Lys Asp Ile Ile Ser Asp Pro Pro Asp
275 280 285Gly Phe Ile Glu Tyr Arg Glu Ala Val Glu Leu Ala Leu Ala Ala Glu
290 295 300Phe Asp Arg Gly Val Pro Thr Ser Trp Asp Arg Ser Trp Thr Val Gln305 310 315 320Gln Pro Trp Ala Gly Gln Pro Thr Asp Pro Glu Trp Ala Gly Lys Ala
325 330 335Val Tyr Glu Asp Val Arg Thr Glu Asp Thr Asp Leu Arg Ala Ala Gln
340 345 350Val Trp Pro Ile Ile Glu Gly Leu Gly Gly Val Asn Gly Trp Tyr Ser
355 360 365Ala Pro Leu Leu Trp Arg Leu Arg Gly Ile Ala Asp Arg Leu Ile Gly
370 375 380Gly Pro Gly Leu Gly Gly Arg Arg Asp Pro Arg His Leu Lys Leu Gly385 390 395 400Asp Arg Ile Asp Trp Trp Arg Val Thr Glu Ile Asp Pro Pro His Arg
405 410 415Leu Val Leu Thr Ala Glu Met Lys Val Asp Gly Gly Ala Trp Leu Ile
420 425 430Leu Glu Val Ala Asp Lys Glu Asn Gly Gly Cys Thr Tyr Thr Gln Arg
435 440 445Ala Ile Phe Glu Pro Lys Gly Leu Pro Gly Tyr Leu Tyr Trp Trp Val
450 455 460Val Ser Pro Phe His Ala Ile Ile Phe Pro Tyr Met Arg Ser Asn Ile465 470 475 480Leu Lys Ala Ala Arg Lys Leu Thr
485<210>699<241>1587<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1564)<223>FRXA00483<400>699agacccaaga gtaaaatccc aggatttgct tatacttgcg ctcatggata atcaacttcg 60tcccactttg cattatcaag ctcaaaaccc gcaccggcga gtg ctg gtc acc ggt 115
Val Leu Val Thr Gly1 5gcg aca ggc tac att ggc ggc agg ttg att act gag tta ctt gct gcc 163Ala Thr Gly Tyr Ile Gly Gly Arg Leu Ile Thr Glu Leu Leu Ala Ala
10 15 20ggt ttc caa gtt cgg gcc acc tcg agg aaa aaa aca agt ctt cag cgc 211Gly Phe Gln Val Arg Ala Thr Ser Arg Lys Lys Thr Ser Leu Gln Arg
25 30 35ttt gac tgg tac gag gac gtc gag gca gtg gaa gcg gat ctg act gac 259Phe Asp Trp Tyr Glu Asp Val Glu Ala Val Glu Ala Asp Leu Thr Asp
40 45 50gcg act gag tta gat acg tta ttt aag gat gta gac gtt gtt tac tat 307Ala Thr Glu Leu Asp Thr Leu Phe Lys Asp Val Asp Val Val Tyr Tyr
55 60 65cta gtg cat tcc atg gga ggt aag aat gtt gat ttt gaa gag caa gag 355Leu Val His Ser Met Gly Gly Lys Asn Val Asp Phe Glu Glu Gln Glu70 75 80 85caa cgc act gct gaa aat gta att caa gct gct gat caa gcc ggg ata 403Gln Arg Thr Ala Glu Asn Val Ile Gln Ala Ala Asp Gln Ala Gly Ile
90 95 100aaa cag att gtc tac ctt tcc ggc tta cac ccg cgt aat cga aaa ata 451Lys Gln Ile Val Tyr Leu Ser Gly Leu His Pro Arg Asn Arg Lys Ile
105 110 115gaa gaa cta tct aag cac atg cgc tca cgg gaa aag gtc gcc cag att 499Glu Glu Leu Ser Lys His Met Arg Ser Arg Glu Lys Val Ala Gln Ile
120 125 130ttg ctg gca ggc cag aca cca gct tta att tta agg gct gcc aca att 547Leu Leu Ala Gly Gln Thr Pro Ala Leu Ile Leu Arg Ala Ala Thr Ile
135 140 145att ggt tcc ggc tct gca tca ttt gaa ata atc cgt cat ctc acg gag 595Ile Gly Ser Gly Ser Ala Ser Phe Glu Ile Ile Arg His Leu Thr Glu150 155 160 165cgt ttg cct aga atg ata gcg cct cag tgg att act aat cag att gag 643Arg Leu Pro Arg Met Ile Ala Pro Gln Trp Ile Thr Asn Gln Ile Glu
170 175 180cct tta gca ata cgg gat gtt ttg cat tac cta atc tcg gcg gct gat 691Pro Leu Ala Ile Arg Asp Val Leu His Tyr Leu Ile Ser Ala Ala Asp
185 190 195tta aag gat cca gtc aac cgc tcc tgc gat att ggg tgt gga aag tcg 739Leu Lys Asp Pro Val Asn Arg Ser Cys Asp Ile Gly Cys Gly Lys Ser
200 205 210tat gaa ttt gcg gat cta ttg cgt atc tat gcc gat gtt cgg gga ctg 787Tyr Glu Phe Ala Asp Leu Leu Arg Ile Tyr Ala Asp Val Arg Gly Leu
215 220 225aaa cgt cat gta aat tcc gta cct ctc aat ttg ccc atg gac aag cta 835Lys Arg His Val Asn Ser Val Pro Leu Asn Leu Pro Met Asp Lys Leu230 235 240 245tcc ggt ctt tgg att agt cta gtg aca cct gtt cca ttt caa ttg tct 883Ser Gly Leu Trp Ile Ser Leu Val Thr Pro Val Pro Phe Gln Leu Ser
250 255 260ttc cct tta gct caa tca atg gct gag gat gcc gtc act gaa gag cac 931Phe Pro Leu Ala Gln Ser Met Ala Glu Asp Ala Val Thr Glu Glu His
265 270 275agc att aaa gat att att tca gat cca ccc gat ggt ttt att gag tat 979Ser Ile Lys Asp Ile Ile Ser Asp Pro Pro Asp Gly Phe Ile Glu Tyr
280 285 290cgg gaa gca gtg gag ctg gca tta gct gca gaa ttt gat cgt gga gtt 1027Arg Glu Ala Val Glu Leu Ala Leu Ala Ala Glu Phe Asp Arg Gly Val
295 300 305cca acg tca tgg gat cga agc tgg act gta caa caa ccg tgg gct ggc 1075Pro Thr Ser Trp Asp Arg Ser Trp Thr Val Gln Gln Pro Trp Ala Gly310 315 320 325cag cct acc gat cca gag tgg gcg ggc aaa gct gta tat gaa gac gtc 1123Gln Pro Thr Asp Pro Glu Trp Ala Gly Lys Ala Val Tyr Glu Asp Val
330 335 340cgc aca gaa gat act gat ctc cga gca gcg cag gtc tgg ccg atc att 1171Arg Thr Glu Asp Thr Asp Leu Arg Ala Ala Gln Val Trp Pro Ile Ile
345 350 355gaa ggt ttg ggt ggc gtg aac ggc tgg tat tct gca cca ctg cta tgg 1219Glu Gly Leu Gly Gly Val Asn Gly Trp Tyr Ser Ala Pro Leu Leu Trp
360 365 370cga ttg cgg ggt atc gct gac aga ctc atc ggc ggt cca ggt ttg ggc 1267Arg Leu Arg Gly Ile Ala Asp Arg Leu Ile Gly Gly Pro Gly Leu Gly
375 380 385gga cgg cgg gat ccc cgt cat ttg aaa ctt ggg gat cgc att gat tgg 1315Gly Arg Arg Asp Pro Arg His Leu Lys Leu Gly Asp Arg Ile Asp Trp390 395 400 405tgg cgg gtt act gag atc gat cca cca cat aga tta gtg ctc acc gca 1363Trp Arg Val Thr Glu Ile Asp Pro Pro His Arg Leu Val Leu Thr Ala
410 415 420gag atg aaa gta gat ggt ggc gct tgg ctg atc ctg gaa gtt gcg gac 1411Glu Met Lys Val Asp Gly Gly Ala Trp Leu Ile Leu Glu Val Ala Asp
425 430 435aag gaa aat ggc gga tgt act tat scc cag cgc gca ata ttt gag ccg 1459Lys Glu Asn Gly Gly Cys Thr Tyr Thr Gln Arg Ala Ile Phe Glu Pro
440 445 450aag ggt ttg ccc ggt tat ctc tat tgg tgg gtt gtt tca ccg ttc cat 1507Lys Gly Leu Pro Gly Tyr Leu Tyr Trp Trp Val Val Ser Pro Phe His
455 460 465gcg att att ttt cct tat atg cgt tcg aat att tta aaa gct gcg cgt 1555Ala Ile Ile Phe Pro Tyr Met Arg Ser Asn Ile Leu Lys Ala Ala Arg470 475 480 485aaa ctc act taatcgcaga gtaggcgtct aaa 1587Lys Leu Thr<210>700<211>488<212>PRT<213>谷氨酸棒杆菌<400>700Val Leu Val Thr Gly Ala Thr Gly Tyr Ile Gly Gly Arg Leu Ile Thr1 5 10 15Glu Leu Leu Ala Ala Gly Phe Gln Val Arg Ala Thr Ser Arg Lys Lys
20 25 30Thr Ser Leu Gln Arg Phe Asp Trp Tyr Glu Asp Val Glu Ala Val Glu
35 40 45Ala Asp Leu Thr Asp Ala Thr Glu Leu Asp Thr Leu Phe Lys Asp Val
50 55 60Asp Val Val Tyr Tyr Leu Val His Ser Met Gly Gly Lys Asn Val Asp65 70 75 80Phe Glu Glu Gln Glu Gln Arg Thr Ala Glu Asn Val Ile Gln Ala Ala
85 90 95Asp Gln Ala Gly Ile Lys Gln Ile Val Tyr Leu Ser Gly Leu His Pro
100 105 110Arg Asn Arg Lys Ile Glu Glu Leu Ser Lys His Met Arg Ser Arg Glu
115 120 125Lys Val Ala Gln Ile Leu Leu Ala Gly Gln Thr Pro Ala Leu Ile Leu
130 135 140Arg Ala Ala Thr Ile Ile Gly Ser Gly Ser Ala Ser Phe Glu Ile Ile145 150 155 160Arg His Leu Thr Glu Arg Leu Pro Arg Met Ile Ala Pro Gln Trp Ile
165 170 175Thr Asn Gln Ile Glu Pro Leu Ala Ile Arg Asp Val Leu His Tyr Leu
180 185 190Ile Ser Ala Ala Asp Leu Lys Asp Pro Val Asn Arg Ser Cys Asp Ile
195 200 205Gly Cys Gly Lys Ser Tyr Glu Phe Ala Asp Leu Leu Arg Ile Tyr Ala
210 215 220Asp Val Arg Gly Leu Lys Arg His Val Asn Ser Val Pro Leu Asn Leu225 230 235 240Pro Met Asp Lys Leu Ser Gly Leu Trp Ile Ser Leu Val Thr Pro Val
245 250 255Pro Phe Gln Leu Ser Phe Pro Leu Ala Gln Ser Met Ala Glu Asp Ala
260 265 270Val Thr Glu Glu His Ser Ile Lys Asp Ile Ile Ser Asp Pro Pro Asp
275 280 285Gly Phe Ile Glu Tyr Arg Glu Ala Val Glu Leu Ala Leu Ala Ala Glu
290 295 300Phe Asp Arg Gly Val Pro Thr Ser Trp Asp Arg Ser Trp Thr Val Gln305 310 315 320Gln Pro Trp Ala Gly Gln Pro Thr Asp Pro Glu Trp Ala Gly Lys Ala
325 330 335Val Tyr Glu Asp Val Arg Thr Glu Asp Thr Asp Leu Arg Ala Ala Gln
340 345 350Val Trp Pro Ile Ile Glu Gly Leu Gly Gly Val Asn Gly Trp Tyr Ser
355 360 365Ala Pro Leu Leu Trp Arg Leu Arg Gly Ile Ala Asp Arg Leu Ile Gly
370 375 380Gly Pro Gly Leu Gly Gly Arg Arg Asp Pro Arg His Leu Lys Leu Gly385 390 395 400Asp Arg Ile Asp Trp Trp Arg Val Thr Glu Ile Asp Pro Pro His Arg
405 410 415Leu Val Leu Thr Ala Glu Met Lys Val Asp Gly Gly Ala Trp Leu Ile
420 425 430Leu Glu Val Ala Asp Lys Glu Asn Gly Gly Cys Thr Tyr Thr Gln Arg
435 440 445Ala Ile Phe Glu Pro Lys Gly Leu Pro Gly Tyr Leu Tyr Trp Trp Val
450 455 460Val Ser Pro Phe His Ala Ile Ile Phe Pro Tyr Met Arg Ser Asn Ile465 470 475 480Leu Lys Ala Ala Arg Lys Leu Thr
485<210>701<211>612<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(589)<223>RXA01534<400>701attgctctca tcggttcgat atagactgaa ttgtctaggt taatatccaa tatggaagaa 60aaactagaca gttaagtaga ctgaatggcc tactaggtgc atg act tca gca atc 115
Met Thr Ser Ala Ile
1 5acc acc gca act gat ctt cgc tcc gta ctg cga aac gta cca acc cca 163Thr Thr Ala Thr Asp Leu Arg Ser Val Leu Arg Asn Val Pro Thr Pro
10 15 20att agc ttc att gca acc cac acc gat cag cct ctg ggc atg atc gtt 211Ile Ser Phe Ile Ala Thr His Thr Asp Gln Pro Leu Gly Met Ile Val
25 30 35ggt tca ttc gtc agc att agc gcc gaa cca cca ttg gta ggc atc ttc 259Gly Ser Phe Val Ser Ile Ser Ala Glu Pro Pro Leu Val Gly Ile Phe
40 45 50ttg cag aag agc tct tct tca tgg cca gct atc gag cag gca tta gtt 307Leu Gln Lys Ser Ser Ser Ser Trp Pro Ala Ile Glu Gln Ala Leu Val
55 60 65acc ggc caa gag cta ggc att tct atc ctc ggc ggg gca cac gca gac 355Thr Gly Gln Glu Leu Gly Ile Ser Ile Leu Gly Gly Ala His Ala Asp70 75 80 85cat gtg cgt aag ctt tct ggc cca tcc gac cag cgc ttt gaa aac ctt 403His Val Arg Lys Leu Ser Gly Pro Ser Asp Gln Arg Phe Glu Asn Leu
90 95 100ggg tgg gca tcc acc gaa aac ggt gcg att cac ctt gsa ggc gct gat 451Gly Trp Ala Ser Thr Glu Asn Gly Ala Ile His Leu Glu Gly Ala Asp
105 110 115gca caa cta acc acg aaa ctt cat gat ctc cag gaa atc ggc gat cac 499Ala Gln Leu Thr Thr Lys Leu His Asp Leu Gln Glu Ile Gly Asp His
120 125 130ttc ttt gca gtt cta gaa gtt att gac gct tcc gct gac caa gac ttc 547Phe Phe Ala Val Leu Glu Val Ile Asp Ala Ser Ala Asp Gln Asp Phe
135 140 145agc tca gcg ctg gtg tac cac cgc tca cag gtg tcc tcg ctg 589Ser Ser Ala Leu Val Tyr His Arg Ser Gln Val Ser Ser Leu150 155 160taggacacta aattttaaga ggg 612<210>702<211>163<212>PRT<213>谷氨酸棒杆菌<400>702Met Thr Ser Ala Ile Thr Thr Ala Thr Asp Leu Arg Ser Val Leu Arg1 5 10 15Asn Val Pro Thr Pro Ile Ser Phe Ile Ala Thr His Thr Asp Gln Pro
20 25 30Leu Gly Met Ile Val Gly Ser Phe Val Ser Ile Ser Ala Glu Pro Pro
35 40 45Leu Val Gly Ile Phe Leu Gln Lys Ser Ser Ser Ser Trp Pro Ala Ile
50 55 60Glu Gln Ala Leu Val Thr Gly Gln Glu Leu Gly Ile Ser Ile Leu Gly65 70 75 80Gly Ala His Ala Asp His Val Arg Lys Leu Ser Gly Pro Ser Asp Gln
85 90 95Arg Phe Glu Asn Leu Gly Trp Ala Ser Thr Glu Asn Gly Ala Ile His
100 105 110Leu Glu Gly Ala Asp Ala Gln Leu Thr Thr Lys Leu His Asp Leu Gln
115 120 125Glu Ile Gly Asp His Phe Phe Ala Val Leu Glu Val Ile Asp Ala Ser
130 135 140Ala Asp Gln Asp Phe Ser Ser Ala Leu Val Tyr His Arg Ser Gln Val145 150 155 160Ser Ser Leu<210>703<211>1134<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1111)<223>RXA00288<400>703ggcgtgctaa aaaagcacgt caaatacaga atcggcagat tacatcgctg agcagagaaa 60acacgggcat gaaacgtacc caagggctaa catcgggggc atg agc gcg caa atg 115
Met Ser Ala Gln Met
1 5gat acc cct gat ccc act atg tct gct gtt gca atg tta gat tcc atc 163Asp Thr Pro Asp Pro Thr Met Ser Ala Val Ala Met Leu Asp Ser Ile
10 15 20cct tct gat caa cca gat ttc ctg atc gat gta gsa gta gat cga cca 211Pro Ser Asp Gln Pro Asp Phe Leu Ile Asp Val Glu Val Asp Arg Pro
25 30 35act ccc gga cca cat gat ctg cta gtc cac att gag gcg gtc tca att 259Thr Pro Gly Pro His Asp Leu Leu Val His Ile Glu Ala Val Ser Ile
40 45 50aac cct gtt gat acc aag gta cgc atg cgg gcc ggg aag caa aag cat 307Asn Pro Val Asp Thr Lys Val Arg Met Arg Ala Gly Lys Gln Lys His
55 60 65cct aaa att tta ggt ttt gat gct gca ggt gag gtg gtg gct gtt gga 355Pro Lys Ile Leu Gly Phe Asp Ala Ala Gly Glu Val Val Ala Val Gly70 75 80 85tcg cag gtc acg ctc ttc aat gtt ggt gac aaa gtg ttc tac gca gga 403Ser Gln Val Thr Leu Phe Asn Val Gly Asp LVs Val Phe Tyr Ala Gly
90 95 100tcc aat cag cgt cca gga agt aac gca gag tac cag gtg gtg gat gaa 451Ser Asn Gln Arg Pro Gly Ser Asn Ala Glu Tyr Gln Val Val Asp Glu
105 110 115cgg ctg gtg ggt cac gca cca caa agc ttg ggg gca cac gac gcc gct 499Arg Leu Val Gly His Ala Pro Gln Ser Leu Gly Ala His Asp Ala Ala
120 125 130gct ctc cca ctt gtc gcg ctc act gca tgg gag tca ctt ttt gac cga 547Ala Leu Pro Leu Val Ala Leu Thr Ala Trp Glu Ser Leu Phe Asp Arg
135 140 145ttg gga gta act cag tca act act gga aca ctg ttg gtc ttg ggc ggt 595Leu Gly Val Thr Gln Ser Thr Thr Gly Thr Leu Leu Val Leu Gly Gly150 155 160 165tca gga ggt gtg cct tca gct ctt att caa ctt gct cga gct ctc act 643Ser Gly Gly Val Pro Ser Ala Leu Ile Gln Leu Ala Arg Ala Leu Thr
170 175 180ggt ctg aaa gta gtg gca aca gct tct cgc cct gaa tca caa gaa tgg 691Gly Leu Lys Val Val Ala Thr Ala Ser Arg Pro Glu Ser Gln Glu Trp
185 190 195gtg aca aag ctc ggt gct cat gag gtg att gat cac tcc aag gat ttg 739Val Thr Lys Leu Gly Ala His Glu Val Ile Asp His Ser Lys Asp Leu
200 205 210agt gag caa atc tcc gac gtg gat ttt gtt ttc agc tcg tgg act act 787Ser Glu Gln Ile Ser Asp Val Asp Phe Val Phe Ser Ser Trp Thr Thr
215 220 225ggg cgt gaa gta gag ctc gcc acg ttg atg aaa ccc cag tcc cac cta 835Gly Arg Glu Val Glu Leu Ala Thr Leu Met Lys Pro Gln Ser His Leu230 235 240 245gtg ctc atc gat gat cea gtg gat ccc aat ttg ggc gct ttt aag caa 883Val Leu Ile Asp Asp Pro Val Asp Pro Asn Leu Gly Ala Phe Lys Gln
250 255 260aaa gcg atc gct ttg cac tgg gag ttc atg ttt acc cgc gct atg ttc 931Lys Ala Ile Ala Leu His Trp Glu Phe Met Phe Thr Arg Ala Met Phe
265 270 275aac act cct gat atg ggt gaa caa ggg aaa att ctg aat aag atc gcc 979Asn Thr Pro Asp Met Gly Glu Gln Gly Lys Ile Leu Asn Lys Ile Ala
280 285 290gac atg gtt gat cgg ggt cag ttt gag tcc gtg aca gca acg gtg ctg 1027Asp Met Val Asp Arg Gly Gln Phe Glu Ser Val Thr Ala Thr Val Leu
295 300 305gat ggg ctc aac gct gca aac atc atg gag ggg cac cgg ctc gtt gag 1075Asp Gly Leu Asn Ala Ala Asn Ile Met Glu Gly His Arg Leu Val Glu310 315 320 325cag ggt aaa acc tca gga aaa att gtt gtg agg gta taaagaggac 1121Gln Gly Lys Thr Ser Gly Lys Ile Val Val Arg Val
330 335ttgaaaaatg cac 1134<210>704<211>337<212>PRT<213>谷氨酸棒杆菌<400>704Met Ser Ala Gln Met Asp Thr Pro Asp Pro Thr Met Ser Ala Val Ala1 5 10 15Met Leu Asp Ser Ile Pro Ser Asp Gln Pro Asp Phe Leu Ile Asp Val
20 25 30Glu Val Asp Arg Pro Thr Pro Gly Pro His Asp Leu Leu Val His Ile
35 40 45Glu Ala Val Ser Ile Asn Pro Val Asp Thr Lys Val Arg Met Arg Ala
50 55 60Gly Lys Gln Lys His Pro Lys Ile Leu Gly Phe Asp Ala Ala Gly Glu65 70 75 80Val Val Ala Val Gly Sar Gln Val Thr Leu Phe Asn Val Gly Asp Lys
85 90 95Val Phe Tyr Ala Gly Ser Asn Gln Arg Pro Gly Ser Asn Ala Glu Tyr
100 105 110Gln Val Val Asp Glu Arg Leu Val Gly His Ala Pro Gln Ser Leu Gly
115 120 125Ala His Asp Ala Ala Ala Leu Pro Leu Val Ala Leu Thr Ala Trp Glu
130 135 140Ser Leu Phe Asp Arg Leu Gly Val Thr Gln Ser Thr Thr Gly Thr Leu145 150 155 160Leu Val Leu Gly Gly Ser Gly Gly Val Pro Ser Ala Leu Ile Gln Leu
165 170 175Ala Arg Ala Leu Thr Gly Leu Lys Val Val Ala Thr Ala Ser Arg Pro
180 185 190Glu Ser Gln Glu Trp Val Thr Lys Leu Gly Ala His Glu Val Ile Asp
195 200 205His Ser Lys Asp Leu Ser Glu Gln Ile Ser Asp Val Asp Phe Val Phe
210 215 220Ser Ser Trp Thr Thr Gly Arg Glu Val Glu Leu Ala Thr Leu Met Lys225 230 235 240Pro Gln Ser His Leu Val Leu Ile Asp Asp Pro Val Asp Pro Asn Leu
245 250 255Gly Ala Phe Lys Gln Lys Ala Ile Ala Leu His Trp Glu Phe Met Phe
260 265 270Thr Arg Ala Met Phe Asn Thr Pro Asp Met Gly Glu Gln Gly Lys Ile
275 280 285Leu Asn Lys Ile Ala Asp Met Val Asp Arg Gly Gln Phe Glu Ser Val
290 295 300Thr Ala Thr Val Leu Asp Gly Leu Asn Ala Ala Asn Ile Met Glu Gly305 310 315 320His Arg Leu Val Glu Gln Gly Lys Thr Ser Gly Lys Ile Val Val Arg
325 330 335Val<210>705<21l>1089<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1066)<223>RXA02741<400>705actggtcacc tggtttggtc tgcactctga ctcccctcaa aagggcacaa tttggtcaat 60ttcccaacct tgtctttcag tcatggttag tgtgggaacc atg aag gca atc tta 115
Met Lys Ala Ile Leu
1 5gtt tcc cgc acc ggc gga cca gag gtg ttg gag ttc acc gac act gac 163Val Ser Arg Thr Gly Gly Pro Glu Val Leu Glu Phe Thr Asp Thr Asp
10 15 20gcc cca aag ccc act gat gat cag gtt tta gtt gaa gtt gat atg gct 211Ala Pro Lys Pro Thr Asp Asp Gln Val Leu Val Glu Val Asp Met Ala
25 30 35ggc gtc aac ttt att gat act tac tat cgc cag ggt gaa tat cac gct 259Gly Val Asn Phe Ile Asp Thr Tyr Tyr Arg Gln Gly Glu Tyr His Ala
40 45 50cgc ctg ccg ttt atc cca ggt ttt gaa ggc act ggt cgg gtg ttg gag 307Arg Leu Pro Phe Ile Pro Gly Phe Glu Gly Thr Gly Arg Val Leu Glu
55 60 65gat ccg cag ggg ttg att gcg gcg ggt acc aag gtg gcg tgg tgt gat 355Asp Pro Gln Gly Leu Ile Ala Ala Gly Thr Lys Val Ala Trp Cys Asp70 75 80 85gcc atg ggt tcg tat gct cag cag gtg tgt gtg ccg cgg gat cgc ttg 403Ala Met Gly Ser Tyr Ala Gln Gln Val Cys Val Pro Arg Asp Arg Leu
90 95 100gtg gcg gtt ccc gag ggc gtg agt tcg gaa gtg gct gcg tcg atg ttg 451Val Ala Val Pro Glu Gly Val Ser Ser Glu Val Ala Ala Ser Met Leu
105 110 115atg cag gga atc act gcg cat tat cta acc aat ggt gtg tat gag ctt 499Met Gln Gly Ile Thr Ala His Tyr Leu Thr Asn Gly Val Tyr Glu Leu
120 125 130gaa gag ggc gat tct tgc ctc atc act gct ggc gcg ggt ggt gtt gga 547Glu Glu Gly Asp Ser Cys Leu Ile Thr Ala Gly Ala Gly Gly Val Gly
135 140 145ttg ttg gct acg cag atg gcg gcg gcc aag gga gtg cgc gtg tac agc 595Leu Leu Ala Thr Gln Met Ala Ala Ala Lys Gly Val Arg Val Tyr Ser150 155 160 165gtg gtg tcc acg gat gaa aaa gct gag ctt gct ttg gat gcc ggt gct 643Val Val Ser Thr Asp Glu Lys Ala Glu Leu Ala Leu Asp Ala Gly Ala
170 175 180tat gag gtg ttt cgt tat tcc gat aat ttg gcg gag cag gtt cgt cgg 691Tyr Glu Val Phe Arg Tyr Ser Asp Asn Leu Ala Glu Gln Val Arg Arg
185 190 195cac aac ggg ggt cgc gga gtt gat gtg gtg tat gac ggt gtc ggc cag 739His Asn Gly Gly Arg Gly Val Asp Val Val Tyr Asp Gly Val Gly Gln
200 205 210tcc acg ttc aat gag tcc tta gag gct gtt cgt ccg cgc ggc act gtg 787Ser Thr Phe Asn Glu Ser Leu Glu Ala Val Arg Pro Arg Gly Thr Val
215 220 225tgt ttg ttt ggt gcg gcg tcg ggt cct gtg gag cct ttt gat ccg cag 835Cys Leu Phe Gly Ala Ala Ser Gly Pro Val Glu Pro Phe Asp Pro Gln230 235 240 245ctg ttg aac act cac ggt tcg atc ttc ttg acc cgc cca agc att ggc 883Leu Leu Asn Thr His Gly Ser Ile Phe Leu Thr Arg Pro Ser Ile Gly
250 255 260gcg tgg acg tct gag gag ggc gaa ttt gcc aag cgt gca cag gcg gtc 931Ala Trp Thr Ser Glu Glu Gly Glu Phe Ala Lys Arg Ala Gln Ala Val
265 270 275acg cag gcc atc gtc gaa ggc acc ttg cgg gtt cgc gtt act ggc aca 979Thr Gln Ala Ile Val Glu Gly Thr Leu Arg Val Arg Val Thr Gly Thr
280 285 290tat tcg ctt gcc gac gcc tac atc gcc cac cgc gac ctt cag gcg cgt 1027Tyr Ser Leu Ala Asp Ala Tyr Ile Ala His Arg Asp Leu Gln Ala Arg
295 300 305agc acg agc ggt tct ttg gtc ttg gaa atc ccg aag gac taaacacgca 1076Ser Thr Ser Gly Ser Leu Val Leu Glu Ile Pro Lys Asp310 315 320taaaaagatc ctg 1089<210>706<211>322<212>PRT<213>谷氨酸棒杆菌<400>706Met Lys Ala Ile Leu Val Ser Arg Thr Gly Gly Pro Glu Val Leu Glu1 5 10 15Phe Thr Asp Thr Asp Ala Pro Lys Pro Thr Asp Asp Gln Val Leu Val
20 25 30Glu Val Asp Met Ala Gly Val Asn Phe Ile Asp Thr Tyr Tyr Arg Gln
35 40 45Gly Glu Tyr His Ala Arg Leu Pro Phe Ile Pro Gly Phe Glu Gly Thr
50 55 60Gly Arg Val Leu Glu Asp Pro Gln Gly Leu Ile Ala Ala Gly Thr Lys65 70 75 80Val Ala Trp Cys Asp Ala Met Gly Ser Tyr Ala Gln Gln Val Cys Val
85 90 95Pro Arg Asp Arg Leu Val Ala Val Pro Glu Gly Val Ser Ser Glu Val
100 105 110Ala Ala Ser Met Leu Met Gln Gly Ile Thr Ala His Tyr Leu Thr Asn
115 120 125Gly Val Tyr Glu Leu Glu Glu Gly Asp Ser Cys Leu Ile Thr Ala Gly
130 135 140Ala Gly Gly Val Gly Leu Leu Ala Thr Gln Met Ala Ala Ala Lys Gly145 150 155 160Val Arg Val Tyr Ser Val Val Ser Thr Asp Glu Lys Ala Glu Leu Ala
165 170 175Leu Asp Ala Gly Ala Tyr Glu Val Phe Arg Tyr Ser Asp Asn Leu Ala
180 185 190Glu Gln Val Arg Arg His Asn Gly Gly Arg Gly Val Asp Val Val Tyr
195 200 205Asp Gly Val Gly Gln Ser Thr Phe Asn Glu Ser Leu Glu Ala Val Arg
210 215 220Pro Arg Gly Thr Val Cys Leu Phe Gly Ala Ala Ser Gly Pro Val Glu225 230 235 240Pro Phe Asp Pro Gln Leu Leu Asn Thr His Gly Ser Ile Phe Leu Thr
245 250 255Arg Pro Ser Ile Gly Ala Trp Thr Ser Glu Glu Gly Glu Phe Ala Lys
260 265 270Arg Ala Gln Ala Val Thr Gln Ala Ile Val Glu Gly Thr Leu Arg Val
275 280 285Arg Val Thr Gly Thr Tyr Ser Leu Ala Asp Ala Tyr Ile Ala His Arg
290 295 300Asp Leu Gln Ala Arg Ser Thr Ser Gly Ser Leu Val Leu Glu Ile Pro305 310 315 320Lys Asp<210>707<211>990<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(967)<223>RXN02560<400>707ttggggcaag ccagctaacg catttcttgt ggaaaccgca gacattgagg ccgcccacgc 60ggaacttcta agagcagtgg aatgaaataa tccggtgctg atg cag ggc aac tcg 115
Met Gln Gly Asn Ser
1 5ctt aat ctg gca gac aac agc gag aga aag aag ccc atg ccg tca cca 163Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys Pro Met Pro Ser Pro
10 15 20gga gaa ctt tta gcc gcc cgc tac gga caa cct gca acc tgg acg cca 211Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro Ala Thr Trp Thr Pro
25 30 35ccg cag tgg aat gag acg ctt gat gtc att cac cag cat cga tca gtt 259Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His Gln His Arg Ser Val
40 45 50cgc agg tgg ttg gat aaa ccg gtt gat gat gac acc atc cgc acc att 307Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp Thr Ile Arg Thr Ile
55 60 65att tcc gcc gca caa tcg gct gga acc tct tcc aat aag cag gtc att 355Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser Asn Lys Gln Val Ile70 75 80 85tct gtc atc gtg gtt aaa gat cct gag ctg agg aaa ggc ctc gcg ggg 403Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg Lys Gly Leu Ala Gly
90 95 100atc act cgc cag atg ttt ccg cac ctt gag cag gtt ccc gcg gtg ctg 451Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln Val Pro Ala Val Leu
105 110 115att tgg ttg att gat tat tcc cga atc agt gcg gtg gca gcc aga gaa 499Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala Val Ala Ala Arg Glu
120 125 130gat ctc cca aca ggg gct ctt gat tat ctc gat gag gcc gcg tgg ggg 547Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp Glu Ala Ala Trp Gly
135 140 145ttc ctc gac gcc gga atc gca gct caa aac gct gca att gct gcg gag 595Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala Ala Ile Ala Ala Glu150 155 160 165tca ctt gga ttg gga acg ctc tat ttg ggt tcg gtg cgc aac gat gcg 643Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser Val Arg Asn Asp Ala
170 175 180gaa gcc gtg cac aaa ttg ctt ggc ctt cca cct gag atc gtg cct gtc 691Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro Glu Ile Val Pro Val
185 190 195gtg ggc ttg gaa atg ggg cat gcg gat ccg cct gaa cct gcc gga att 739Val Gly Leu Glu Met Gly His Ala Asp Pro Pro Glu Pro Ala Gly Ile
200 205 210aaa cct ccc ctg cca caa gaa gcc att gtt cac tgg gat acc tac acc 787Lys Pro Pro Leu Pro Gln Glu Ala Ile Val His Trp Asp Thr Tyr Thr
215 220 225gag aaa aac ctc gaa ctt atc gat tcc tac gac cgc gcc ctc gac act 835Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp Arg Ala Leu Asp Thr230 235 240 245tac tat tct cgc tac ggc cag cac cag ctc tgg tcg aag cag acg gcg 883Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp Ser Lys Gln Thr Ala
250 255 260cat agg gcg gcg tcg aaa agc ttt tca aaa acc aac agg cag ttc ctt 931His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr Asn Arg Gln Phe Leu
265 270 275agg ggc gtg ttt gag cgc gcc ggg ttt ggg ctg aga taaaagcatg 977Arg Gly Val Phe Glu Arg Ala Gly Phe Gly Leu Arg
280 285attatggacg cct 990<210>708<211>289<212>PRT<213>谷氨酸棒杆菌<400> 708Met Gln Gly Asn Ser Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys1 5 10 15Pro Met Pro Ser Pro Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro
20 25 30Ala Thr Trp Thr Pro Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His
35 40 45Gln His Arg Ser Val Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp
50 55 60Thr Ile Arg Thr Ile Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser65 70 75 80Asn Lys Gln Val Ile Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg
85 90 95Lys Gly Leu Ala Gly Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln
100 105 110Val Pro Ala Val Leu Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala
115 120 125Val Ala Ala Arg Glu Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp
130 135 140Glu Ala Ala Trp Gly Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala145 150 155 160Ala Ile Ala Ala Glu Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser
165 170 175Val Arg Asn Asp Ala Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro
180 185 190Glu Ile Val Pro Val Val Gly Leu Glu Met Gly His Ala Asp Pro Pro
195 200 205Glu Pro Ala Gly Ile Lys Pro Pro Leu Pro Gln Glu Ala Ile Val His
210 215 220Trp Asp Thr Tyr Thr Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp225 230 235 240Arg Ala Leu Asp Thr Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp
245 250 255Ser Lys Gln Thr Ala His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr
260 265 270Asn Arg Gln Phe Leu Arg Gly Val Phe Glu Arg Ala Gly Phe Gly Leu
275 280 285Arg<210>709<211>922<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(922)<223>FRXA02560<400>709ttggggcaag ccagctaacg catttcttgt ggaaaccgca gacattgagg ccgcccacgc 60ggaacttcta agagcagtgg aatgaaataa tccggtgctg atg cag ggc aac tcg 115
Met Gln Gly Asn Ser
1 5ctt aat ctg gca gac aac agc gag aga aag aag ccc atg ccg tca cca 163Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys Pro Met Pro Ser Pro
10 15 20gga gaa ctt tta gcc gcc cgc tac gga caa cct gca acc tgg acg cca 211Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro Ala Thr Trp Thr Pro
25 30 35ccg cag tgg aat gag acg ctt gat gtc att cac cag cat cga tca gtt 259Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His Gln His Arg Ser Val
40 45 50cgc agg tgg ttg gat aaa ccg gtt gat gat gac acc atc cgc acc att 307Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp Thr Ile Arg Thr Ile
55 60 65att tcc gcc gca caa tcg gct gga acc tct tcc aat aag cag gtc att 355Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser Asn Lys Gln Val Ile70 75 80 85tct gtc atc gtg gtt aaa gat cct gag ctg agg aaa ggc ctc gcg ggg 403Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg Lys Gly Leu Ala Gly
90 95 100atc act cgc cag atg ttt ccg cac ctt gag cag gtt ccc gcg gtg ctg 451Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln Val Pro Ala Val Leu
105 110 115att tgg ttg att gat tat tcc cga atc agt gcg gtg gca gcc aga gaa 499Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala Val Ala Ala Arg Glu
120 125 130gat ctc cca aca ggg gct ctt gat tat ctc gat gag gcc gcg tgg ggg 547Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp Glu Ala Ala Trp Gly
135 140 145ttc ctc gac gcc gga atc gca gct caa aac gct gca att gct gcg gag 595Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala Ala Ile Ala Ala Glu150 155 160 165tca ctt gga ttg gga acg ctc tat ttg ggt tcg gtg cgc aac gat gcg 643Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser Val Arg Asn Asp Ala
170 175 180gaa gcc gtg cac aaa ttg ctt ggc ctt cca cct gag atc gtg cct gtc 691Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro Glu Ile Val Pro Val
185 190 195gtg ggc ttg gaa atg ggg cat gcg gat ccg cct gaa cct gcc gga att 739Val Gly Leu Glu Met Gly His Ala Asp Pro Pro Glu Pro Ala Gly Ile
200 205 210aaa cct ccc ctg cca caa gaa gcc att gtt cac tgg gat acc tac acc 787Lys Pro Pro Leu Pro Gln Glu Ala Ile Val His Trp Asp Thr Tyr Thr
215 220 225gag aaa aac ctc gaa ctt atc gat tcc tac gac cgc gcc ctc gac act 835Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp Arg Ala Leu Asp Thr230 235 240 245tac tat tct cgc tac ggc cag cac cag ctc tgg tcg aag cag acg gcg 883Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp Ser Lys Gln Thr Ala
250 255 260cat agg gcg gcg tct aaa cgc tgt tac aag aac caa gaa 922His Arg Ala Ala Ser Lys Arg Cys Tyr Lys Asn Gln Glu
265 270<210>710<211>274<212>PRT<213>谷氨酸棒杆菌<400> 710Met Gln Gly Asn Ser Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys1 5 10 15Pro Met Pro Ser Pro Gly Glu Leu Leu Ala Ala Arg Tyr Gly Gln Pro
20 25 30Ala Thr Trp Thr Pro Pro Gln Trp Asn Glu Thr Leu Asp Val Ile His
35 40 45Gln His Arg Ser Val Arg Arg Trp Leu Asp Lys Pro Val Asp Asp Asp
50 55 60Thr Ile Arg Thr Ile Ile Ser Ala Ala Gln Ser Ala Gly Thr Ser Ser65 70 75 80Asn Lys Gln Val Ile Ser Val Ile Val Val Lys Asp Pro Glu Leu Arg
85 90 95Lys Gly Leu Ala Gly Ile Thr Arg Gln Met Phe Pro His Leu Glu Gln
100 105 110Val Pro Ala Val Leu Ile Trp Leu Ile Asp Tyr Ser Arg Ile Ser Ala
115 120 125Val Ala Ala Arg Glu Asp Leu Pro Thr Gly Ala Leu Asp Tyr Leu Asp
130 135 140Glu Ala Ala Trp Gly Phe Leu Asp Ala Gly Ile Ala Ala Gln Asn Ala145 150 155 160Ala Ile Ala Ala Glu Ser Leu Gly Leu Gly Thr Leu Tyr Leu Gly Ser
165 170 175Val Arg Asn Asp Ala Glu Ala Val His Lys Leu Leu Gly Leu Pro Pro
180 185 190Glu Ile Val Pro Val Val Gly Leu Glu Met Gly His Ala Asp Pro Pro
195 200 205Glu Pro Ala Gly Ile Lys Pro Pro Leu Pro Gln Glu Ala Ile Val His
210 215 220Trp Asp Thr Tyr Thr Glu Lys Asn Leu Glu Leu Ile Asp Ser Tyr Asp225 230 235 240Arg Ala Leu Asp Thr Tyr Tyr Ser Arg Tyr Gly Gln His Gln Leu Trp
245 250 255Ser Lys Gln Thr Ala His Arg Ala Ala Ser Lys Arg Cys Tyr Lys Asn
260 265 270Gln Glu<210>711<211>870<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(847)<223>RXA01311<400>711tcgtctccgc atgggaacca ggcgagaatg gaaccttcgt ctgccacgca gaaccactgt 60tcttcgaatc tgtcccactg cagacaagga actacaagta atg aaa ctt aca ctt 115
Met Lys Leu Thr Leu
1 5gag atc tgg cgt caa gca ggc cca act gcg gaa ggc aag ttc gaa acc 163Glu Ile Trp Arg Gln Ala Gly Pro Thr Ala Glu Gly Lys Phe Glu Thr
10 15 20gtc cag gtt gac gac gcc gtc gcg cag atg tcc atc ctg gag ctg ctt 211Val Gln Val Asp Asp Ala Val Ala Gln Met Ser Ile Leu Glu Leu Leu
25 30 35gac cac gta aac aac aag ttc atc gaa gaa ggc aaa gaa cca ttc gcg 259Asp His Val Asn Asn Lys Phe Ile Glu Glu Gly Lys Glu Pro Phe Ala
40 45 50ttc gcc tct gac tgc cgc gaa ggc att tgt ggt acc tgt ggt ctc ctc 307Phe Ala Ser Asp Cys Arg Glu Gly Ile Cys Gly Thr Cys Gly Leu Leu
55 60 65gtg aac ggt cgc cct cac ggc gcc gac cag aac aag cct gcc tgt gcg 355Val Asn Gly Arg Pro His Gly Ala Asp Gln Asn Lys Pro Ala Cys Ala70 75 80 85cag cgc ctg gtc agc tac aag gaa ggc gac acc ctc aag atc gaa cca 403Gln Arg Leu Val Ser Tyr Lys Glu Gly Asp Thr Leu Lys Ile Glu Pro
90 95 100ctg cgt tcc gcc gca tac cca gtg atc aag gac atg gtc gtc gac cgc 451Leu Arg Ser Ala Ala Tyr Pro Val Ile Lys Asp Met Val Val Asp Arg
105 110 115tcc gca ctg gac cgt gtc atg gaa cag ggt ggc tac gtg acc atc aac 499Ser Ala Leu Asp Arg Val Met Glu Gln Gly Gly Tyr Val Thr Ile Asn
120 125 130gca ggt acc gca cct gac gct gat acc ctc cac gtc aac cac gaa acc 547Ala Gly Thr Ala Pro Asp Ala Asp Thr Leu His Val Asn His Glu Thr
135 140 145gca gaa ctc gca ctt gac cac gca gcc tgc atc ggc tgt ggc gca tgt 595Ala Glu Leu Ala Leu Asp His Ala Ala Cys Ile Gly Cys Gly Ala Cys150 155 160 165gtt gct gcc tgc cct aac ggc gca gca cac ctg ttc acc ggc gca aag 643Val Ala Ala Cys Pro Asn Gly Ala Ala His Leu Phe Thr Gly Ala Lys
170 175 180ctt gtt cac ctc tcc ctc ctc cca ctg ggt aag gaa gag cgc gga ctg 691Leu Val His Leu Ser Leu Leu Pro Leu Gly Lys Glu Glu Arg Gly Leu
185 190 195cgt gca cgt aag atg gtt gat gaa atg gaa acc aac ttc gga cac tgc 739Arg Ala Arg Lys Met Val Asp Glu Met Glu Thr Asn Phe Gly His Cys
200 205 210tcc ctc tac ggc gag tgc gca gat gtc tgc ccc gca ggc atc cca ctg 787Ser Leu Tyr Gly Glu Cys Ala Asp Val Cys Pro Ala Gly Ile Pro Leu
215 220 225acc gct gtg gca gct gtc acc aag gaa cgt gcg cgt gca gct ttc cga 835Thr Ala Val Ala Ala Val Thr Lys Glu Arg Ala Arg Ala Ala Phe Arg230 235 240 245ggc aaa gac gac tagtctttaa tccaagtaag tac 870Gly Lys Asp Asp<210>712<211>249<212>PRT<213>谷氨酸棒杆菌<400> 712Met Lys Leu Thr Leu Glu Ile Trp Arg Gln Ala Gly Pro Thr Ala Glu1 5 10 15Gly Lys Phe Glu Thr Val Gln Val Asp Asp Ala Val Ala Gln Met Ser
20 25 30Ile Leu Glu Leu Leu Asp His Val Asn Asn Lys Phe Ile Glu Glu Gly
35 40 45Lys Glu Pro Phe Ala Phe Ala Ser Asp Cys Arg Glu Gly Ile Cys Gly
50 55 60Thr Cys Gly Leu Leu Val Asn Gly Arg Pro His Gly Ala Asp Gln Asn65 70 75 80Lys Pro Ala Cys Ala Gln Arg Leu Val Ser Tyr Lys Glu Gly Asp Thr
85 90 95Leu Lys Ile Glu Pro Leu Arg Ser Ala Ala Tyr Pro Val Ile Lys Asp
100 105 110Met Val Val Asp Arg Ser Ala Leu Asp Arg Val Met Glu Gln Gly Gly
115 120 125Tyr Val Thr Ile Asn Ala Gly Thr Ala Pro Asp Ala Asp Thr Leu His
130 135 140Val Asn His Glu Thr Ala Glu Leu Ala Leu Asp His Ala Ala Cys Ile145 150 155 160Gly Cys Gly Ala Cys Val Ala Ala Cys Pro Asn Gly Ala Ala His Leu
165 170 175Phe Thr Gly Ala Lys Leu Val His Leu Ser Leu Leu Pro Leu Gly Lys
180 185 190Glu Glu Arg Gly Leu Arg Ala Arg Lys Met Val Asp Glu Met Glu Thr
195 200 205Asn Phe Gly His Cys Ser Leu Tyr Gly Glu Cys Ala Asp Val Cys Pro
210 215 220Ala Gly Ile Pro Leu Thr Ala Val Ala Ala Val Thr Lys Glu Arg Ala225 230 235 240Arg Ala Ala Phe Arg Gly Lys Asp Asp
245<210>713<211>929<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(906)<223>RXN03014<400>713tac gtt ggt ttc gaa gtg ctg ctg gtg gcg tca tac gtg ctg ctc acc 48Tyr Val Gly Phe Glu Val Leu Leu Val Ala Ser Tyr Val Leu Leu Thr1 5 10 15ttg ggt gca tcg ccg gca cgt gta cgt tcc ggc gtg ggt tac gtg atg 96Leu Gly Ala Ser Pro Ala Arg Val Arg Ser Gly Val Gly Tyr Val Met
20 25 30gtg tcc atg gcg tca tcg atg gtg ttc ctg ttt gga ctc gca atg gtt 144Val Ser Met Ala Ser Ser Met Val Phe Leu Phe Gly Leu Ala Met Val
35 40 45tac gcc tca gtg ggc acg ttg aac atg gct cac gtt ggc cta cgc atg 192Tyr Ala Ser Val Gly Thr Leu Asn Met Ala His Val Gly Leu Arg Met
50 55 60gaa gat gtt ccg tct gga act cgc tcc gcg atc ttc gca gtg ttg ctc 240Glu Asp Val Pro Ser Gly Thr Arg Ser Ala Ile Phe Ala Val Leu Leu65 70 75 80gtg gca ttc ggt att aaa gct gcc gtg ttc ccc cta gat tcc tgg ctg 288Val Ala Phe Gly Ile Lys Ala Ala Val Phe Pro Leu Asp Ser Trp Leu
85 90 95ccg gac tcc tac ccc acc gcg cca tcg ctg gtc acc gcg gtg ttc gca 336Pro Asp Ser Tyr Pro Thr Ala Pro Ser Leu Val Thr Ala Val Phe Ala
100 105 110ggt ctg ttg acc aag gtg ggt gtg tat tcc atc att cga gca cgc tcg 384Gly Leu Leu Thr Lys Val Gly Val Tyr Ser Ile Ile Arg Ala Arg Ser
115 120 125att att ttc acc gat gga tcc ctt gac acc atg ctg atg tgg gtg gca 432Ile Ile Phe Thr Asp Gly Ser Leu Asp Thr Met Leu Met Trp Val Ala
130 135 140ctc gcc acc atg ctc att ggt att ttg ggc gcg atg gcg caa aac gat 480Leu Ala Thr Met Leu Ile Gly Ile Leu Gly Ala Met Ala Gln Asn Asp145 150 155 160atc aaa cgt ttg ttg tca ttt act ctg gtc agc cac atc ggc tac atg 528Ile Lys Arg Leu Leu Ser Phe Thr Leu Val Ser His Ile Gly Tyr Met
165 170 175atc ttc ggc gta gcc ctt gga tct gca cag ggt ttg tct ggt gcg atc 576Ile Phe Gly Val Ala Leu Gly Ser Ala Gln Gly Leu Ser Gly Ala Ile
180 185 190ttc tac gca atc cac cac att ctg gtt cag act tcc ctg ttc ctg gtg 624Phe Tyr Ala Ile His His Ile Leu Val Gln Thr Ser Leu Phe Leu Val
195 200 205gtc ggt ctg gtg gaa cgc caa gcc gga tcc tcc tcg ctg cga cgc ctt 672Val Gly Leu Val Glu Arg Gln Ala Gly Ser Ser Ser Leu Arg Arg Leu
210 215 220gga tcc ctg gca tat atc tcc cca ctt ctt gcg att ttg tac ttc atc 720Gly Ser Leu Ala Tyr Ile Ser Pro Leu Leu Ala Ile Leu Tyr Phe Ile225 230 235 240ccc gcc atc aac ctg ggt ggt atc cca ccg ttc tcc ggc ttc ctg ggc 768Pro Ala Ile Asn Leu Gly Gly Ile Pro Pro Phe Ser Gly Phe Leu Gly
245 250 255aag atc atg ctc atc gaa gcc ggc gcc cga aga tgg cag ttg gct ggc 816Lys Ile Met Leu Ile Glu Ala Gly Ala Arg Arg Trp Gln Leu Ala Gly
260 265 270atg ggt cct tat cgc agg cgc cgt tgt cac ctc act gct cac ctt gta 864Met Gly Pro Tyr Arg Arg Arg Arg Cys His Leu Thr Ala His Leu Val
275 280 285cac cat ggt tct ggt ctg gtc caa ggc ctt ctg gcg cga ccg 906His His Gly Ser Gly Leu Val Gln Gly Leu Leu Ala Arg Pro
290 295 300taaagacgcc cccgatggag caa 929<210>714<211>302<212>PRT<213>谷氨酸棒杆菌<400> 714Tyr Val Gly Phe Glu Val Leu Leu Val Ala Ser Tyr Val Leu Leu Thr1 5 10 15Leu Gly Ala Ser Pro Ala Arg Val Arg Ser Gly Val Gly Tyr Val Met
20 25 30Val Ser Met Ala Ser Ser Met Val Phe Leu Phe Gly Leu Ala Met Val
35 40 45Tyr Ala Ser Val Gly Thr Leu Asn Met Ala His Val Gly Leu Arg Met
50 55 60Glu Asp Val Pro Ser Gly Thr Arg Ser Ala Ile Phe Ala Val Leu Leu65 70 75 80Val Ala Phe Gly Ile Lys Ala Ala Val Phe Pro Leu Asp Ser Trp Leu
85 90 95Pro Asp Ser Tyr Pro Thr Ala Pro Ser Leu Val Thr Ala Val Phe Ala
100 105 110Gly Leu Leu Thr Lys Val Gly Val Tyr Ser Ile Ile Arg Ala Arg Ser
115 120 125Ile Ile Phe Thr Asp Gly Ser Leu Asp Thr Met Leu Met Trp Val Ala
130 135 140Leu Ala Thr Met Leu Ile Gly Ile Leu Gly Ala Met Ala Gln Asn Asp145 150 155 160Ile Lys Arg Leu Leu Ser Phe Thr Leu Val Ser His Ile Gly Tyr Met
165 170 175Ile Phe Gly Val Ala Leu Gly Ser Ala Gln Gly Leu Ser Gly Ala Ile
180 185 190Phe Tyr Ala Ile His His Ile Leu Val Gln Thr Ser Leu Phe Leu Val
195 200 205Val Gly Leu Val Glu Arg Gln Ala Gly Ser Ser Ser Leu Arg Arg Leu
210 215 220Gly Ser Leu Ala Tyr Ile Ser Pro Leu Leu Ala Ile Leu Tyr Phe Ile225 230 235 240Pro Ala Ile Asn Leu Gly Gly Ile Pro Pro Phe Ser Gly Phe Leu Gly
245 250 255Lys Ile Met Leu Ile Glu Ala Gly Ala Arg Arg Trp Gln Leu Ala Gly
260 265 270Met Gly Pro Tyr Arg Arg Arg Arg Cys His Leu Thr Ala His Leu Val
275 280 285His His Gly Ser Gly Leu Val Gln Gly Leu Leu Ala Arg Pro
290 295 300<210>715<211>1280<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(1257)<223>FRXA00910<400>715tac gtt ggt ttc gaa gtg ctg ctg gtg gcg tca tac gtg ctg ctc acc 48Tyr Val Gly Phe Glu Val Leu Leu Val Ala Ser Tyr Val Leu Leu Thr1 5 10 15ttg ggt gca tcg ccg gca cgt gta cgt tcc ggc gtg ggt tac gtg atg 96Leu Gly Ala Ser Pro Ala Arg Val Arg Ser Gly Val Gly Tyr Val Met
20 25 30gtg tcc atg gcg tca tcg atg gtg ttc ctg ttt gga ctc gca atg gtt 144Val Ser Met Ala Ser Ser Met Val Phe Leu Phe Gly Leu Ala Met Val
35 40 45tac gcc tca gtg ggc acg ttg aac atg gct cac gtt ggc cta cgc atg 192Tyr Ala Ser Val Gly Thr Leu Asn Met Ala His Val Gly Leu Arg Met
50 55 60gaa gat gtt ccg tct gga act cgc tcc gcg atc ttc gca gtg ttg ctc 240Glu Asp Val Pro Ser Gly Thr Arg Ser Ala Ile Phe Ala Val Leu Leu65 70 75 80gtg gca ttc ggt att aaa gct gcc gtg ttc ccc cta gat tcc tgg ctg 288Val Ala Phe Gly Ile Lys Ala Ala Val Phe Pro Leu Asp Ser Trp Leu
85 90 95ccg gac tcc tac ccc acc gcg cca tcg ctg gtc acc gcg gtg ttc gca 336Pro Asp Ser Tyr Pro Thr Ala Pro Ser Leu Val Thr Ala Val Phe Ala
100 105 110ggt ctg ttg acc aag gtg ggt gtg tat tcc atc att cga gca cgc tcg 384Gly Leu Leu Thr Lys Val Gly Val Tyr Ser Ile Ile Arg Ala Arg Ser
115 120 125att att ttc acc gat gga tcc ctt gac acc atg ctg atg tgg gtg gca 432Ile Ile Phe Thr Asp Gly Ser Leu Asp Thr Met Leu Met Trp Val Ala
130 135 140ctc gcc acc atg ctc att ggt att ttg ggc gcg atg gcg caa aac gat 480Leu Ala Thr Met Leu Ile Gly Ile Leu Gly Ala Met Ala Gln Asn Asp145 150 155 160atc aaa cgt ttg ttg tca ttt act ctg gtc agc cac atc ggc tac atg 528Ile Lys Arg Leu Leu Ser Phe Thr Leu Val Ser His Ile Gly Tyr Met
165 170 175atc ttc ggc gta gcc ctt gga tct gca cag ggt ttg tct ggt gcg atc 576Ile Phe Gly Val Ala Leu Gly Ser Ala Gln Gly Leu Ser Gly Ala Ile
180 185 190ttc tac gca atc cac cac att ctg gtt cag act tcc ctg ttc ctg gtg 624Phe Tyr Ala Ile His His Ile Leu Val Gln Thr Ser Leu Phe Leu Val
195 200 205gtc ggt ctg gtg gaa cgc caa gcc gga tcc tcc tcg ctg cga cgc ctt 672Val Gly Leu Val Glu Arg Gln Ala Gly Ser Ser Ser Leu Arg Arg Leu
210 215 220gga tcc ctg gca tat atc tcc cca ctt ctt gcg att ttg tac ttc atc 720Gly Ser Leu Ala Tyr Ile Ser Pro Leu Leu Ala Ile Leu Tyr Phe Ile225 230 235 240ccc gcc atc aac ctg ggt ggt atc cca ccg ttc tcc ggc ttc ctg ggc 768Pro Ala Ile Asn Leu Gly Gly Ile Pro Pro Phe Ser Gly Phe Leu Gly
245 250 255aag atc atg ctc atc gaa gcc ggc gcc gaa gat ggc agt tgg ctg gca 816Lys Ile Met Leu Ile Glu Ala Gly Ala Glu Asp Gly Ser Trp Leu Ala
260 265 270tgg gtc ctt atc gca ggc gcc gtt gtc acc tca ctg ctc acc ttg tac 864Trp Val Leu Ile Ala Gly Ala Val Val Thr Ser Leu Leu Thr Leu Tyr
275 280 285acc atg gtt ctg gtc tgg tcc aag gcc ttc tgg cgc gac cgt aaa gac 912Thr Met Val Leu Val Trp Ser Lys Ala Phe Trp Arg Asp Arg Lys Asp
290 295 300gcc ccc gat gga gca acc gca ctt gcg cga ccc gca cct ttg gta gat 960Ala Pro Asp Gly Ala Thr Ala Leu Ala Arg Pro Ala Pro Leu Val Asp305 310 315 320gtc caa gac gaa gtc gcc gtt aaa gac cgc aac gat gtc gga cgg atg 1008Val Gln Asp Glu Val Ala Val Lys Asp Arg Asn Asp Val Gly Arg Met
325 330 335cct tgg ggc atg gtc ttc tcc act gct ctc ctg gtt tca gca tct ctt 1056Pro Trp Gly Met Val Phe Ser Thr Ala Leu Leu Val Ser Ala Ser Leu
340 345 350gct gta tcc gtg ctc gca gga cca ctg tca tct att act gga cgc gcc 1104Ala Val Ser Val Leu Ala Gly Pro Leu Ser Ser Ile Thr Gly Arg Ala
355 360 365gcc gaa tcc gca caa gat gtc aac atc tac cgc gcc gca gta ctc ggc 1152Ala Glu Ser Ala Gln Asp Val Asn Ile Tyr Arg Ala Ala Val Leu Gly
370 375 380ccc aac tac ctc gac cca tca cgc aca ctc gag atg gag cgt tac gac 1200Pro Asn Tyr Leu Asp Pro Ser Arg Thr Leu Glu Met Glu Arg Tyr Asp385 390 395 400gcc aac cgc gat gac atc aac cac cgc gtc gac acc aac gga acg gag 1248Ala Asn Arg Asp Asp Ile Asn His Arg Val Asp Thr Asn Gly Thr Glu
405 410 415gac caa cca tgatcagtgg attcaaacga cga 1280Asp Gln Pro<210>716<211>419<212>PRT<213>谷氨酸棒杆菌<400>716Tyr Val Gly Phe Glu Val Leu Leu Val Ala Ser Tyr Val Leu Leu Thr1 5 10 15Leu Gly Ala Ser Pro Ala Arg Val Arg Ser Gly Val Gly Tyr Val Met
20 25 30Val Ser Met Ala Ser Ser Met Val Phe Leu Phe Gly Leu Ala Met Val
35 40 45Tyr Ala Ser Val Gly Thr Leu Asn Met Ala His Val Gly Leu Arg Met
50 55 60Glu Asp Val Pro Ser Gly Thr Arg Ser Ala Ile Phe Ala Val Leu Leu65 70 75 80Val Ala Phe Gly Ile Lys Ala Ala Val Phe Pro Leu Asp Ser Trp Leu
85 90 95Pro Asp Ser Tyr Pro Thr Ala Pro Ser Leu Val Thr Ala Val Phe Ala
100 105 110Gly Leu Leu Thr Lys Val Gly Val Tyr Ser Ile Ile Arg Ala Arg Ser
115 120 125Ile Ile Phe Thr Asp Gly Ser Leu Asp Thr Met Leu Met Trp Val Ala
130 135 140Leu Ala Thr Met Leu Ile Gly Ile Leu Gly Ala Met Ala Gln Asn Asp145 150 155 160Ile Lys Arg Leu Leu Ser Phe Thr Leu Val Ser His Ile Gly Tyr Met
165 170 175Ile Phe Gly Val Ala Leu Gly Ser Ala Gln Gly Leu Ser Gly Ala Ile
180 185 190Phe Tyr Ala Ile His His Ile Leu Val Gln Thr Ser Leu Phe Leu Val
195 200 205Val Gly Leu Val Glu Arg Gln Ala Gly Ser Ser Ser Leu Arg Arg Leu
210 215 220Gly Ser Leu Ala Tyr Ile Ser Pro Leu Leu Ala Ile Leu Tyr Phe Ile225 230 235 240Pro Ala Ile Asn Leu Gly Gly Ile Pro Pro Phe Ser Gly Phe Leu Gly
245 250 255Lys Ile Met Leu Ile Glu Ala Gly Ala Glu Asp Gly Ser Trp Leu Ala
260 265 270Trp Val Leu Ile Ala Gly Ala Val Val Thr Ser Leu Leu Thr Leu Tyr
275 280 285Thr Met Val Leu Val Trp Ser Lys Ala Phe Trp Arg Asp Arg Lys Asp
290 295 300Ala Pro Asp Gly Ala Thr Ala Leu Ala Arg Pro Ala Pro Leu Val Asp305 310 315 320Val Gln Asp Glu Val Ala Val Lys Asp Arg Asn Asp Val Gly Arg Met
325 330 335Pro Trp Gly Met Val Phe Ser Thr Ala Leu Leu Val Ser Ala Ser Leu
340 345 350Ala Val Ser Val Leu Ala Gly Pro Leu Ser Ser Ile Thr Gly Arg Ala
355 360 365Ala Glu Ser Ala Gln Asp Val Asn Ile Tyr Arg Ala Ala Val Leu Gly
370 375 380Pro Asn Tyr Leu Asp Pro Ser Arg Thr Leu Glu Met Glu Arg Tyr Asp385 390 395 400Ala Asn Arg Asp Asp Ile Asn His Arg Val Asp Thr Asn Gly Thr Glu
405 410 415Asp Gln Pro<210>717<211>1051<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1051)<223>RXN01895<400>717cgcgtacacg tgctcaacac gacaacgctt aaacggctgc acgcgtaaca cggcagaccg 60cacaagcttt aagatccacg atcaggagac tttgacaaat atg tca gtt aac cca 115
Met Ser Val Asn Pro
1 5acc cgc ccc gaa ggc ggc cgt cac cac gtc gtc gtc atc ggt tct ggt 163Thr Arg Pro Glu Gly Gly Arg His His Val Val Val Ile Gly Ser Gly
10 15 20ttt ggt ggc ctt ttt gct gcc aag aac ctg gcc aag gca gac gtc gat 211Phe Gly Gly Leu Phe Ala Ala Lys Asn Leu Ala Lys Ala Asp Val Asp
25 30 35gtc act ctg att gac cgc acc aac cac cac ctc ttc cag cca ctg ctg 259Val Thr Leu Ile Asp Arg Thr Asn His His Leu Phe Gln Pro Leu Leu
40 45 50tac caa gtg gca acc ggt atc ctc tcc tcc ggt gaa atc gca cct tcc 307Tyr Gln Val Ala Thr Gly Ile Leu Ser Ser Gly Glu Ile Ala Pro Ser
55 60 65act cga cag atc ctg ggc tcc cag gaa aac gtc aac gtc atc aag ggc 355Thr Arg Gln Ile Leu Gly Ser Gln Glu Asn Val Asn Val Ile Lys Gly70 75 80 85gaa gtc acc gac atc aac gtc gag tcc cag act gtg acc gcc tcc ctg 403Glu Val Thr Asp Ile Asn Val Glu Ser Gln Thr Val Thr Ala Ser Leu
90 95 100ggc gag ttc acc cgc gtt ttt gag tac gat tcc ttg gtc gtt ggt gct 451Gly Glu Phe Thr Arg Val Phe Glu Tyr Asp Ser Leu Val Val Gly Ala
105 110 115ggc gca ggt cag tcc tac ttc ggc aat gat cac ttc gct gag ttc gca 499Gly Ala Gly Gln Ser Tyr Phe Gly Asn Asp His Phe Ala Glu Phe Ala
120 125 130cct ggc atg aag tcc atc gac gat gca ctg gag att cgt gca cgc atc 547Pro Gly Met Lys Ser Ile Asp Asp Ala Leu Glu Ile Arg Ala Arg Ile
135 140 145atc ggt gct ttc gag cgc gct gag atc tgc gag gat cca gct gag cgc 595Ile Gly Ala Phe Glu Arg Ala Glu Ile Cys Glu Asp Pro Ala Glu Arg150 155 160 165gaa cgc ctg ctc acc ttc gtc gtt gtt ggc gct ggc cca acc ggt gtt 643Glu Arg Leu Leu Thr Phe Val Val Val Gly Ala Gly Pro Thr Gly Val
170 175 180gag ctt gct ggc cag ttg gct gag atg gct cac cgc acc ctt gct ggt 691Glu Leu Ala Gly Gln Leu Ala Glu Met Ala His Arg Thr Leu Ala Gly
185 190 195gag tac aag aac ttc aac acc aac tcc gca aag atc atc ctg ctt gat 739Glu Tyr Lys Asn Phe Asn Thr Asn Ser Ala Lys Ile Ile Leu Leu Asp
200 205 210ggt gct cca cag gtt ctt cct cca ttc ggt aag cgc cta ggc cgc aac 787Gly Ala Pro Gln Val Leu Pro Pro Phe Gly Lys Arg Leu Gly Arg Asn
215 220 225gca cag cgc acc ctg gaa aag atg ggt gtc aac gtt cgc ctg aac gct 835Ala Gln Arg Thr Leu Glu Lys Met Gly Val Asn Val Arg Leu Asn Ala230 235 240 245atg gtc acc aac gtt gac gct acc tcg gtc acc tac aag acc aag gac 883Met Val Thr Asn Val Asp Ala Thr Ser Val Thr Tyr Lys Thr Lys Asp
250 255 260ggc gaa gag cac acc atc gaa tct ttc tgc aag att tgg tcc gct ggt 931Gly Glu Glu His Thr Ile Glu Ser Phe Cys Lys Ile Trp Ser Ala Gly
265 270 275gtt gcg gca tcc cca ctg ggc aag ctc gtc gca gag cag acc ggt gtt 979Val Ala Ala Ser Pro Leu Gly Lys Leu Val Ala Glu Gln Thr Gly Val
280 285 290gag acc gac cgc gca ggc cgc gtc atg gtt aac gat gac ctg tct gtt 1027Glu Thr Asp Arg Ala Gly Arg Val Met Val Asn Asp Asp Leu Ser Val
295 300 305ggc gat cag aag aac gtc ttc gtt 1051Gly Asp Gln Lys Asn Val Phe Val310 315<210>718<211>317<212>PRT<213>谷氨酸棒杆菌<400>718Met Ser Val Asn Pro Thr Arg Pro Glu Gly Gly Arg His His Val Val1 5 10 15Val Ile Gly Ser Gly Phe Gly Gly Leu Phe Ala Ala Lys Asn Leu Ala
20 25 30Lys Ala Asp Val Asp Val Thr Leu Ile Asp Arg Thr Asn His His Leu
35 40 45Phe Gln Pro Leu Leu Tyr Gln Val Ala Thr Gly Ile Leu Ser Ser Gly
50 55 60Glu Ile Ala Pro Ser Thr Arg Gln Ile Leu Gly Ser Gln Glu Asn Val65 70 75 80Asn Val Ile Lys Gly Glu Val Thr Asp Ile Asn Val Glu Ser Gln Thr
85 90 95Val Thr Ala Ser Leu Gly Glu Phe Thr Arg Val Phe Glu Tyr Asp Ser
100 105 110Leu Val Val Gly Ala Gly Ala Gly Gln Ser Tyr Phe Gly Asn Asp His
115 120 125Phe Ala Glu Phe Ala Pro Gly Met Lys Ser Ile Asp Asp Ala Leu Glu
130 135 140Ile Arg Ala Arg Ile Ile Gly Ala Phe Glu Arg Ala Glu Ile Cys Glu145 150 155 160Asp Pro Ala Glu Arg Glu Arg Leu Leu Thr Phe Val Val Val Gly Ala
165 170 175Gly Pro Thr Gly Val Glu Leu Ala Gly Gln Leu Ala Glu Met Ala His
180 185 190Arg Thr Leu Ala Gly Glu Tyr Lys Asn Phe Asn Thr Asn Ser Ala Lys
195 200 205Ile Ile Leu Leu Asp Gly Ala Pro Gln Val Leu Pro Pro Phe Gly Lys
210 215 220Arg Leu Gly Arg Asn Ala Gln Arg Thr Leu Glu Lys Met Gly Val Asn225 230 235 240Val Arg Leu Asn Ala Met Val Thr Asn Val Asp Ala Thr Ser Val Thr
245 250 255Tyr Lys Thr Lys Asp Gly Glu Glu His Thr Ile Glu Ser Phe Cys Lys
260 265 270Ile Trp Ser Ala Gly Val Ala Ala Ser Pro Leu Gly Lys Leu Val Ala
275 280 285Glu Gln Thr Gly Val Glu Thr Asp Arg Ala Gly Arg Val Met Val Asn
290 295 300Asp Asp Leu Ser Val Gly Asp Gln Lys Asn Val Phe Val305 310 315<210>719<211>816<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(816)<223>FRXA01895<400>719cac cac ctc ttc cag cca ctg ctg tac caa gtg gca acc ggt atc ctc 48His His Leu Phe Gln Pro Leu Leu Tyr Gln Val Ala Thr Gly Ile Leu1 5 10 15tcc tcc ggt gaa atc gca cct tcc act cga cag atc ctg ggc tcc cag 96Ser Ser Gly Glu Ile Ala Pro Ser Thr Arg Gln Ile Leu Gly Ser Gln
20 25 30gaa aac gtc aac gtc atc aag ggc gaa gtc acc gac atc aac gtc gag 144Glu Asn Val Asn Val Ile Lys Gly Glu Val Thr Asp Ile Asn Val Glu
35 40 45tcc cag act gtg acc gcc tcc ctg ggc gag ttc acc cgc gtt ttt gag 192Ser Gln Thr Val Thr Ala Ser Leu Gly Glu Phe Thr Arg Val Phe Glu
50 55 60tac gat tcc ttg gtc gtt ggt gct ggc gca ggt cag tcc tac ttc ggc 240Tyr Asp Ser Leu Val Val Gly Ala Gly Ala Gly Gln Ser Tyr Phe Gly65 70 75 80aat gat cac ttc gct gag ttc gca cct ggc atg aag tcc arc gac gat 288Asn Asp His Phe Ala Glu Phe Ala Pro Gly Met Lys Ser Ile Asp Asp
85 90 95gca ctg gag att cgt gca cgc atc arc ggt gct ttc gag cgc gct gag 336Ala Leu Glu Ile Arg Ala Arg Ile Ile Gly Ala Phe Glu Arg Ala Glu
100 105 110atc tgc gag gat cca gct gag cgc gaa cgc ctg ctc acc ttc gtc gtt 384Ile Cys Glu Asp Pro Ala Glu Arg Glu Arg Leu Leu Thr Phe Val Val
115 120 125gtt ggc gct ggc cca acc ggt gtt gag ctt gct ggc cag ttg gct gag 432Val Gly Ala Gly Pro Thr Gly Val Glu Leu Ala Gly Gln Leu Ala Glu
130 135 140atg gct cac cgc acc ctt gct ggt gag tac aag aac ttc aac acc aac 480Met Ala His Arg Thr Leu Ala Gly Glu Tyr Lys Asn Phe Asn Thr Asn145 150 155 160tcc gca aag atc atc ctg ctt gat ggt gct cca cag gtt ctt cct cca 528Ser Ala Lys Ile Ile Leu Leu Asp Gly Ala Pro Gln Val Leu Pro Pro
165 170 175ttc ggt aag cgc cta ggc cgc aac gca cag cgc acc ctg gaa aag atg 576Phe Gly Lys Arg Leu Gly Arg Asn Ala Gln Arg Thr Leu Glu Lys Met
180 185 190ggt gtc aac gtt cgc ctg aac gct atg gtc acc aac gtt gac gct acc 624Gly Val Asn Val Arg Leu Asn Ala Met Val Thr Asn Val Asp Ala Thr
195 200 205tcg gtc acc tac aag acc aag gac ggc gaa gag cac acc atc gaa tct 672Ser Val Thr Tyr Lys Thr Lys Asp Gly Glu Glu His Thr Ile Glu Ser
210 215 220ttc tgc aag att tgg tcc gct ggt gtt gcg gca tcc cca ctg ggc aag 720Phe Cys Lys Ile Trp Ser Ala Gly Val Ala Ala Ser Pro Leu Gly Lys225 230 235 240ctc gtc gca gag cag acc ggt gtt gag acc gac cgc gca ggc cgc gtc 768Leu Val Ala Glu Gln Thr Gly Val Glu Thr Asp Arg Ala Gly Arg Val
245 250 255atg gtt aac gat gac ctg tct gtt ggc gat cag aag aac gtc ttc gtt 816Met Val Asn Asp Asp Leu Ser Val Gly Asp Gln Lys Asn Val Phe Val
260 265 270<210>720<211>272<212>PRT<213>谷氨酸棒杆菌<400>720His His Leu Phe Gln Pro Leu Leu Tyr Gln Val Ala Thr Gly Ile Leu1 5 10 15Ser Ser Gly Glu Ile Ala Pro Ser Thr Arg Gln Ile Leu Gly Ser Gln
20 25 30Glu Asn Val Asn Val Ile Lys Gly Glu Val Thr Asp Ile Asn Val Glu
35 40 45Ser Gln Thr Val Thr Ala Ser Leu Gly Glu Phe Thr Arg Val Phe Glu
50 55 60Tyr Asp Ser Leu Val Val Gly Ala Gly Ala Gly Gln Ser Tyr Phe Gly65 70 75 80Asn Asp His Phe Ala Glu Phe Ala Pro Gly Met Lys Ser Ile Asp Asp
85 90 95Ala Leu Glu Ile Arg Ala Arg Ile Ile Gly Ala Phe Glu Arg Ala Glu
100 105 110Ile Cys Glu Asp Pro Ala Glu Arg Glu Arg Leu Leu Thr Phe Val Val
115 120 125Val Gly Ala Gly Pro Thr Gly Val Glu Leu Ala Gly Gln Leu Ala Glu
130 135 140Met Ala His Arg Thr Leu Ala Gly Glu Tyr Lys Asn Phe Asn Thr Asn145 150 155 160Ser Ala Lys Ile Ile Leu Leu Asp Gly Ala Pro Gln Val Leu Pro Pro
165 170 175Phe Gly Lys Arg Leu Gly Arg Asn Ala Gln Arg Thr Leu Glu Lys Met
180 185 190Gly Val Asn Val Arg Leu Asn Ala Met Val Thr Asn Val Asp Ala Thr
195 200 205Ser Val Thr Tyr Lys Thr Lys Asp Gly Glu Glu His Thr Ile Glu Ser
210 215 220Phe Cys Lys Ile Trp Ser Ala Gly Val Ala Ala Ser Pro Leu Gly Lys225 230 235 240Leu Val Ala Glu Gln Thr Gly Val Glu Thr Asp Arg Ala Gly Arg Val
245 250 255Met Val Asn Asp Asp Leu Ser Val Gly Asp Gln Lys Asn Val Phe Val
260 265 270<210>721<211>2409<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(2386)<223>RXA00703<400>721ctgggagtcc tcttgatttt aggttttcca cataccccca tatagattga agaattcatt 60tttcggcatg ggttcaattg ccgggtctag actgtgacct atg aca acc cct cca 115
Met Thr Thr Pro Pro
1 5act gag att tcg aac gtg aat ccc acc gcg aat gaa ttt gat gat ccg 163Thr Glu Ile Ser Asn Val Asn Pro Thr Ala Asn Glu Phe Asp Asp Pro
10 15 20gat gtg gga cgg cgc att act tct gct gct ggt gtg cca ggc gtt ttg 211Asp Val Gly Arg Arg Ile Thr Ser Ala Ala Gly Val Pro Gly Val Leu
25 30 35cat gcg ctc cag cat gct gtt ccg aat cgt gcc ctg ctg ccg ttg ctc 259His Ala Leu Gln His Ala Val Pro Asn Arg Ala Leu Leu Pro Leu Leu
40 45 50acc atg aat aaa cca ggc ggc atc gac tgt cct ggt tgt gct tgg cct 307Thr Met Asn Lys Pro Gly Gly Ile Asp Cys Pro Gly Cys Ala Trp Pro
55 60 65gag cct tcc act gcc aac ctt ggt gtg gtt gag ttc tgc gag aac ggt 355Glu Pro Ser Thr Ala Asn Leu Gly Val Val Glu Phe Cys Glu Asn Gly70 75 80 85gcc aag gcg gtc gcc gag gaa aca aca cct gat cgt gcc ggc aaa gag 403Ala Lys Ala Val Ala Glu Glu Thr Thr Pro Asp Arg Ala Gly Lys Glu
90 95 100ttc tgg gca gag cat tct att tat gat ctg cgg gaa aag acc gat cac 451Phe Trp Ala Glu His Ser Ile Tyr Asp Leu Arg Glu Lys Thr Asp His
105 110 115tgg ctg gga aag cgt ggc cga atc acc gag ccc atg ttt tat gat cgt 499Trp Leu Gly Lys Arg Gly Arg Ile Thr Glu Pro Met Phe Tyr Asp Arg
120 125 130tct tct ggc gat gat cac tac cgc cct att tct tgg gat cgt gca ttt 547Ser Ser Gly Asp Asp His Tyr Arg Pro Ile Ser Trp Asp Arg Ala Phe
135 140 145gcg atc att gcg tcg aag ctc cgc gag atc gag cca gat gaa gcg gtg 595Ala Ile Ile Ala Ser Lys Leu Arg Glu Ile Glu Pro Asp Glu Ala Val150 155 160 165ttt tac acc tct ggt cga gca ccc aat gag ccg gct tat atg ctg cag 643Phe Tyr Thr Ser Gly Arg Ala Pro Asn Glu Pro Ala Tyr Met Leu Gln
170 175 180ctt cta gcc cgc cga ctt ggc aca aat aat ctt cca gac tgt gga aac 691Leu Leu Ala Arg Arg Leu Gly Thr Asn Asn Leu Pro Asp Cys Gly Asn
185 190 195atg tgc cac gag tcc acc ggt act gcc ttg ggt gag acc ttg ggt ttg 739Met Cys His Glu Ser Thr Gly Thr Ala Leu Gly Glu Thr Leu Gly Leu
200 205 210ggc aag gga tcc gtg gtg atg gag gat ttc tac aac act gat ttg ttg 787Gly Lys Gly Ser Val Val Met Glu Asp Phe Tyr Asn Thr Asp Leu Leu
215 220 225att tcc gtg gga caa aac ccg ggc acc aac cac cca cgt gcg ttg acg 835Ile Ser Val Gly Gln Asn Pro Gly Thr Asn His Pro Arg Ala Leu Thr230 235 240 245gct ttc aaa gaa ttg aag gaa aac ggt ggc aag att ctg gcg ctg aac 883Ala Phe Lys Glu Leu Lys Glu Asn Gly Gly Lys Ile Leu Ala Leu Asn
250 255 260ccc atg cca gag acc ggt ctg atg aaa ttc cgt gag ccc caa tca gtc 931Pro Met Pro Glu Thr Gly Leu Met Lys Phe Arg Glu Pro Gln Ser Val
265 270 275aag ggc gcg ttg agc att tca gac aaa ctt gct gat gaa tac ttg cag 979Lys Gly Ala Leu Ser Ile Ser Asp Lys Leu Ala Asp Glu Tyr Leu Gln
280 285 290atc cgt ctt gat gga gac cgc gca ttc ttc cag gcg ctc aac aag gaa 1027Ile Arg Leu Asp Gly Asp Arg Ala Phe Phe Gln Ala Leu Asn Lys Glu
295 300 305ctc atc cgt aga gat gcc cta gat cat gca ttc ttg gat aaa ttc tgt 1075Leu Ile Arg Arg Asp Ala Leu Asp His Ala Phe Leu Asp Lys Phe Cys310 315 320 325tca ggt gtg gat gaa acc atc gag cac ctc aaa tca ctc gat gat gag 1123Ser Gly Val Asp Glu Thr Ile Glu His Leu Lys Ser Leu Asp Asp Glu
330 335 340gtt ctg ctc aag gga tgc ggt ctg acg gca gcg gag atc aac aag gcc 1171Val Leu Leu Lys Gly Cys Gly Leu Thr Ala Ala Glu Ile Asn Lys Ala
345 350 355gct gac atg gtg gaa aag tct gac acc gtg gtg gtg tca tgg act ctc 1219Ala Asp Met Val Glu Lys Ser Asp Thr Val Val Val Ser Trp Thr Leu
360 365 370ggg gtc acc cag cat aag aac gct gtg tac acc atc cgt gaa atg gtg 1267Gly Val Thr Gln His Lys Asn Ala Val Tyr Thr Ile Arg Glu Met Val
375 380 385aac ttc ctg ctg ctt act gga aat att ggt aag cct ggc gca ggc act 1315Asn Phe Leu Leu Leu Thr Gly Asn Ile Gly Lys Pro Gly Ala Gly Thr390 395 400 405gcc ccg ctt cgt ggg cac tca aac gtc cag ggt gat cga acc atg ggt 1363Ala Pro Leu Arg Gly His Ser Asn Val Gln Gly Asp Arg Thr Met Gly
410 415 420att tgg gag aaa atg ccg gag gca ttc ctt gct gct ctt gaa aac gag 1411Ile Trp Glu Lys Met Pro Glu Ala Phe Leu Ala Ala Leu Glu Asn Glu
425 430 435ttt ggt ttc gat gtg ccc cgc aag cac ggc ttc gac acg gta aat tcc 1459Phe Gly Phe Asp Val Pro Arg Lys His Gly Phe Asp Thr Val Asn Ser
440 445 450ctg cga gcc atg cgc gaa ggc aag acc aag ttc ttt ctc tcc ctc ggt 1507Leu Arg Ala Met Arg Glu Gly Lys Thr Lys Phe Phe Leu Ser Leu Gly
455 460 465ggc aac ctt gtt cga gtg tcc tca gat acg tct gtt gtc gaa aag ggc 1555Gly Asn Leu Val Arg Val Ser Ser Asp Thr Ser Val Val Glu Lys Gly470 475 480 485atg gaa tcc aat gag ctg acg gtg cat ctg tcg acc aag ccc aat ggt 1603Met Glu Ser Asn Glu Leu Thr Val His Leu Ser Thr Lys Pro Asn Gly
490 495 500tca caa gca tgg cct ggt gag cag tca ctt atc ctt ccg gtg att gct 1651Ser Gln Ala Trp Pro Gly Glu Gln Ser Leu Ile Leu Pro Val Ile Ala
505 510 515cga aca gat aag gat gtc caa aag tca ggc gtc cag cgt gtg aca gtt 1699Arg Thr Asp Lys Asp Val Gln Lys Ser Gly Val Gln Arg Val Thr Val
520 525 530gag gat tct gcc ggc gct gtt cac gca tcc act ggt aaa cga acc gcc 1747Glu Asp Ser Ala Gly Ala Val His Ala Ser Thr Gly Lys Arg Thr Ala
535 540 545aac aag gat ctg aat ttg aag tcc gaa tgc gac atc att gga acc atc 1795Asn Lys Asp Leu Asn Leu Lys Ser Glu Cys Asp Ile Ile Gly Thr Ile550 555 560 565ggt aag cag acc ttc ggt gat gcc ttc tgg cag ccg atg att gat aac 1843Gly Lys Gln Thr Phe Gly Asp Ala Phe Trp Gln Pro Met Ile Asp Asn
570 575 580tac gat gtg gtc cgc gat cac atc gag gcc acc att cct ggg ttc cac 1891Tyr Asp Val Val Arg Asp His Ile Glu Ala Thr Ile Pro Gly Phe His
585 590 595gat ttc aac cgt cgc atc gac aac ccc ggt gga ttc ctc ctc ccc aac 1939Asp Phe Asn Arg Arg Ile Asp Asn Pro Gly Gly Phe Leu Leu Pro Asn
600 605 610gga cct cgt gag cgc atc ttc aac aca tcc aat ggc aag gcc caa ttg 1987Gly Pro Arg Glu Arg Ile Phe Asn Thr Ser Asn Gly Lys Ala Gln Leu
615 620 625acg gtt aat gaa acc aat gtg att gag cta ccc aag gac tat ttg ctt 2035Thr Val Asn Glu Thr Asn Val Ile Glu Leu Pro Lys Asp Tyr Leu Leu630 635 640 645atg aac acg gta cgt tca cat gat caa tac aac tcc acg att tac ggt 2083Met Asn Thr Val Arg Ser His Asp Gln Tyr Asn Ser Thr Ile Tyr Gly
650 655 660ctg gat gac cgc tac cgc ggt gtt cgc aat ggt cgc cgc gta gtg ttc 2131Leu Asp Asp Arg Tyr Arg Gly Val Arg Asn Gly Arg Arg Val Val Phe
665 670 675gtc aat cct caa gat tgt aag caa cgt ggt ctc aag gat gga gac atc 2179Val Asn Pro Gln Asp Cys Lys Gln Arg Gly Leu Lys Asp Gly Asp Ile
680 685 690gtc gat atc gtc tct gtc ttt gat gat ggc gaa cgc cga gca ccg aat 2227Val Asp Ile Val Ser Val Phe Asp Asp Gly Glu Arg Arg Ala Pro Asn
695 700 705ttc cga gtg gtg gaa tat gac acc gcg agg gac tgc gtc acc acg tat 2275Phe Arg Val Val Glu Tyr Asp Thr Ala Arg Asp Cys Val Thr Thr Tyr710 715 720 725ttc cct gag gcc aac gta ttg gtt cca ttg gat tca gta gct gaa aaa 2323Phe Pro Glu Ala Asn Val Leu Val Pro Leu Asp Ser Val Ala Glu Lys
730 735 740tcc aac act cca gtg tcc aag tca gtt gtg gtt cgc ctt gaa gca aca 2371Ser Asn Thr Pro Val Ser Lys Ser Val Val Val Arg Leu Glu Ala Thr
745 750 755gga cgt act gct tct tagaaaaaca ccagggaatt ttc 2409Gly Arg Thr Ala Ser
760<210>722<211>762<212>PRT<213>谷氨酸棒杆菌<400>722Met Thr Thr Pro Pro Thr Glu Ile Ser Asn Val Asn Pro Thr Ala Asn1 5 10 15Glu Phe Asp Asp Pro Asp Val Gly Arg Arg Ile Thr Ser Ala Ala Gly
20 25 30Val Pro Gly Val Leu His Ala Leu Gln His Ala Val Pro Asn Arg Ala
35 40 45Leu Leu Pro Leu Leu Thr Met Asn Lys Pro Gly Gly Ile Asp Cys Pro
50 55 60Gly Cys Ala Trp Pro Glu Pro Ser Thr Ala Asn Leu Gly Val Val Glu65 70 75 80Phe Cys Glu Asn Gly Ala Lys Ala Val Ala Glu Glu Thr Thr Pro Asp
85 90 95Arg Ala Gly Lys Glu Phe Trp Ala Glu His Ser Ile Tyr Asp Leu Arg
100 105 110Glu Lys Thr Asp His Trp Leu Gly Lys Arg Gly Arg Ile Thr Glu Pro
115 120 125Met Phe Tyr Asp Arg Ser Ser Gly Asp Asp His Tyr Arg Pro Ile Ser
130 135 140Trp Asp Arg Ala Phe Ala Ile Ile Ala Ser Lys Leu Arg Glu Ile Glu145 150 155 160Pro Asp Glu Ala Val Phe Tyr Thr Ser Gly Arg Ala Pro Asn Glu Pro
165 170 175Ala Tyr Met Leu Gln Leu Leu Ala Arg Arg Leu Gly Thr Asn Asn Leu
180 185 190Pro Asp Cys Gly Asn Met Cys His Glu Ser Thr Gly Thr Ala Leu Gly
195 200 205Glu Thr Leu Gly Leu Gly Lys Gly Ser Val Val Met Glu Asp Phe Tyr
210 215 220Asn Thr Asp Leu Leu Ile Ser Val Gly Gln Asn Pro Gly Thr Asn His225 230 235 240Pro Arg Ala Leu Thr Ala Phe Lys Glu Leu Lys Glu Asn Gly Gly Lys
245 250 255Ile Leu Ala Leu Asn Pro Met Pro Glu Thr Gly Leu Met Lys Phe Arg
260 265 270Glu Pro Gln Ser Val Lys Gly Ala Leu Ser Ile Ser Asp Lys Leu Ala
275 280 285Asp Glu Tyr Leu Gln Ile Arg Leu Asp Gly Asp Arg Ala Phe Phe Gln
290 295 300Ala Leu Asn Lys Glu Leu Ile Arg Arg Asp Ala Leu Asp His Ala Phe305 310 315 320Leu Asp Lys Phe Cys Ser Gly Val Asp Glu Thr Ile Glu His Leu Lys
325 330 335Ser Leu Asp Asp Glu Val Leu Leu Lys Gly Cys Gly Leu Thr Ala Ala
340 345 350Glu Ile Asn Lys Ala Ala Asp Met Val Glu Lys Ser Asp Thr Val Val
355 360 365Val Ser Trp Thr Leu Gly Val Thr Gln His Lys Asn Ala Val Tyr Thr
370 375 380Ile Arg Glu Met Val Asn Phe Leu Leu Leu Thr Gly Asn Ile Gly Lys385 390 395 400Pro Gly Ala Gly Thr Ala Pro Leu Arg Gly His Ser Asn Val Gln Gly
405 410 415Asp Arg Thr Met Gly Ile Trp Glu Lys Met Pro Glu Ala Phe Leu Ala
420 425 430Ala Leu Glu Asn Glu Phe Gly Phe Asp Val Pro Arg Lys His Gly Phe
435 440 445Asp Thr Val Asn Ser Leu Arg Ala Met Arg Glu Gly Lys Thr Lys Phe
450 455 460Phe Leu Ser Leu Gly Gly Asn Leu Val Arg Val Ser Ser Asp Thr Ser465 470 475 480Val Val Glu Lys Gly Met Glu Ser Asn Glu Leu Thr Val His Leu Ser
485 490 495Thr Lys Pro Asn Gly Ser Gln Ala Trp Pro Gly Glu Gln Ser Leu Ile
500 505 510Leu Pro Val Ile Ala Arg Thr Asp Lys Asp Val Gln Lys Ser Gly Val
515 520 525Gln Arg Val Thr Val Glu Asp Ser Ala Gly Ala Val His Ala Ser Thr
530 535 540Gly Lys Arg Thr Ala Asn Lys Asp Leu Asn Leu Lys Ser Glu Cys Asp545 550 555 560Ile Ile Gly Thr Ile Gly Lys Gln Thr Phe Gly Asp Ala Phe Trp Gln
565 570 575Pro Met Ile Asp Asn Tyr Asp Val Val Arg Asp His Ile Glu Ala Thr
580 585 590Ile Pro Gly Phe His Asp Phe Asn Arg Arg Ile Asp Asn Pro Gly Gly
595 600 605Phe Leu Leu Pro Asn Gly Pro Arg Glu Arg Ile Phe Asn Thr Ser Asn
610 615 620Gly Lys Ala Gln Leu Thr Val Asn Glu Thr Asn Val Ile Glu Leu Pro625 630 635 640Lys Asp Tyr Leu Leu Met Asn Thr Val Arg Ser His Asp Gln Tyr Asn
645 650 655Ser Thr Ile Tyr Gly Leu Asp Asp Arg Tyr Arg Gly Val Arg Asn Gly
660 665 670Arg Arg Val Val Phe Val Asn Pro Gln Asp Cys Lys Gln Arg Gly Leu
675 680 685Lys Asp Gly Asp Ile Val Asp Ile Val Ser Val Phe Asp Asp Gly Glu
690 695 700Arg Arg Ala Pro Asn Phe Arg Val Val Glu Tyr Asp Thr Ala Arg Asp705 710 715 720Cys Val Thr Thr Tyr Phe Pro Glu Ala Asn Val Leu Val Pro Leu Asp
725 730 735Ser Val Ala Glu Lys Ser Asn Thr Pro Val Ser Lys Ser Val Val Val
740 745 750Arg Leu Glu Ala Thr Gly Arg Thr Ala Ser
755 760<210>723<211>1038<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1015)<223>RXN00705<400>723gttctggaac aagcactgat gattgggccg agtccacgtt ggttaatgct ctgcatcttc 60aagaaatcat cgctaaaaat tacccggagg ctaaataaaa atg ggt cgg att acc 115
Met Gly Arg Ile Thr
1 5caa aac ttg cag gtc cca cgc gtt gtg tcc act gac gag caa gtt ttt 163Gln Asn Leu Gln Val Pro Arg Val Val Ser Thr Asp Glu Gln Val Phe
10 15 20gtt aac act cgt ccg gat act gtt gcg gtg gag gag cct cta gaa att 211Val Asn Thr Arg Pro Asp Thr Val Ala Val Glu Glu Pro Leu Glu Ile
25 30 35cgg gtt aat ggc act gcg ctt acc acc act atg cgc acg ccc ggc cat 259Arg Val Asn Gly Thr Ala Leu Thr Thr Thr Met Arg Thr Pro Gly His
40 45 50gat att gag ttg gtg cat ggc ctc ctc ttg tca gaa ggt ctg atc acg 307Asp Ile Glu Leu Val His Gly Leu Leu Leu Ser Glu Gly Leu Ile Thr
55 60 65gat gct tct gag gtt ttt acc gcc cgc tat tgt gca gga gct gtt ggc 355Asp Ala Ser Glu Val Phe Thr Ala Arg Tyr Cys Ala Gly Ala Val Gly70 75 80 85cca gat aat caa aat acg tac aac gtc tta gaa ctt gat gtc atc ccc 403Pro Asp Asn Gln Asn Thr Tyr Asn Val Leu Glu Leu Asp Val Ile Pro
90 95 100aaa gac aat ccg gcc cgg gat ccc gtc cag aat ccc tcc cat aat ccc 451Lys Asp Asn Pro Ala Arg Asp Pro Val Gln Asn Pro Ser His Asn Pro
105 110 115gaa ggc agc caa cac gaa gca ctc cac atc cca act ttc caa ccg gta 499Glu Gly Ser Gln His Glu Ala Leu His Ile Pro Thr Phe Gln Pro Val
120 125 130cgc gaa cta aac ctc gtg gca gcc caa cgc aat gtg ctg act acg tct 547Arg Glu Leu Asn Leu Val Ala Ala Gln Arg Asn Val Leu Thr Thr Ser
135 140 145gct tgt ggt gtt tgt ggc acg acg tct att gag cag ttg atg aac aag 595Ala Cys Gly Val Cys Gly Thr Thr Ser Ile Glu Gln Leu Met Asn Lys150 155 160 165aag ggc tgg ccc att acg ccg att aca ccg gat cct cgg atg att gtg 643Lys Gly Trp Pro Ile Thr Pro Ile Thr Pro Asp Pro Arg Met Ile Val
170 175 180tcg ttg cca gat aag ttg aag tcg aag cag aag att ttc gac aaa act 691Ser Leu Pro Asp Lys Leu Lys Ser Lys Gln Lys Ile Phe Asp Lys Thr
185 190 195ggt ggg gtt cat gct gct ggt ttg gcc acg ctt gat ggt gag atg ttg 739Gly Gly Val His Ala Ala Gly Leu Ala Thr Leu Asp Gly Glu Met Leu
200 205 210att att cga gag gat gtc ggt cgg cat aac gca gct gac aaa gtt ata 787Ile Ile Arg Glu Asp Val Gly Arg His Asn Ala Ala Asp Lys Val Ile
215 220 225gga aac atg ctg atg gcg gga aag ctc ccc ttg gaa aac act att ttg 835Gly Asn Met Leu Met Ala Gly Lys Leu Pro Leu Glu Asn Thr Ile Leu230 235 240 245gtg atg agt tct agg gcg tct ttt gag ctt gtc caa aag gct gcc atg 883Val Met Ser Ser Arg Ala Ser Phe Glu Leu Val Gln Lys Ala Ala Met
250 255 260gct gga att tcg ggt gta atc gct gtt ggt gct gca aca tcg ctg gca 931Ala Gly Ile Ser Gly Val Ile Ala Val Gly Ala Ala Thr Ser Leu Ala
265 270 275atc gag gcg gcg cag gat tca ggt att ttc ctt gct ggt ttt gtt cgg 979Ile Glu Ala Ala Gln Asp Ser Gly Ile Phe Leu Ala Gly Phe Val Arg
280 285 290ggc aac aag ttt aac cac tat gcg ggc gag ctc gga taatgccaga 1025Gly Asn Lys Phe Asn His Tyr Ala Gly Glu Leu Gly
295 300 305acaggtagaa cag 1038<210>724<211>305<212>PRT<213>谷氨酸棒杆菌<400> 724Met Gly Arg Ile Thr Gln Asn Leu Gln Val Pro Arg Val Val Ser Thr1 5 10 15Asp Glu Gln Val Phe Val Asn Thr Arg Pro Asp Thr Val Ala Val Glu
20 25 30Glu Pro Leu Glu Ile Arg Val Asn Gly Thr Ala Leu Thr Thr Thr Met
35 40 45Arg Thr Pro Gly His Asp Ile Glu Leu Val His Gly Leu Leu Leu Ser
50 55 60Glu Gly Leu Ile Thr Asp Ala Ser Glu Val Phe Thr Ala Arg Tyr Cys65 70 75 80Ala Gly Ala Val Gly Pro Asp Asn Gln Asn Thr Tyr Asn Val Leu Glu
85 90 95Leu Asp Val Ile Pro Lys Asp Asn Pro Ala Arg Asp Pro Val Gln Asn
100 105 110Pro Ser His Asn Pro Glu Gly Ser Gln His Glu Ala Leu His Ile Pro
115 120 125Thr Phe Gln Pro Val Arg Glu Leu Asn Leu Val Ala Ala Gln Arg Asn
130 135 140Val Leu Thr Thr Ser Ala Cys Gly Val Cys Gly Thr Thr Ser Ile Glu145 150 155 160Gln Leu Met Asn Lys Lys Gly Trp Pro Ile Thr Pro Ile Thr Pro Asp
165 170 175Pro Arg Met Ile Val Ser Leu Pro Asp Lys Leu Lys Ser Lys Gln Lys
180 185 190Ile Phe Asp Lys Thr Gly Gly Val His Ala Ala Gly Leu Ala Thr Leu
195 200 205Asp Gly Glu Met Leu Ile Ile Arg Glu Asp Val Gly Arg His Asn Ala
210 215 220Ala Asp Lys Val Ile Gly Asn Met Leu Met Ala Gly Lys Leu Pro Leu225 230 235 240Glu Asn Thr Ile Leu Val Met Ser Ser Arg Ala Ser Phe Glu Leu Val
245 250 255Gln Lys Ala Ala Met Ala Gly Ile Ser Gly Val Ile Ala Val Gly Ala
260 265 270Ala Thr Ser Leu Ala Ile Glu Ala Ala Gln Asp Ser Gly Ile Phe Leu
275 280 285Ala Gly Phe Val Arg Gly Asn Lys Phe Asn His Tyr Ala Gly Glu Leu
290 295 300Gly305<210>725<211>908<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(885)<223>FRXA00705<400>725cca cgc gtt gtg tcc act gac gag caa gtt ttt gtt aac act cgt ccg 48Pro Arg Val Val Ser Thr Asp Glu Gln Val Phe Val Asn Thr Arg Pro1 5 10 15gat act gtt gcg gtg gag gag cct cta gaa att cgg gtt aat ggc act 96Asp Thr Val Ala Val Glu Glu Pro Leu Glu Ile Arg Val Asn Gly Thr
20 25 30gcg ctt acc acc act atg cgc acg ccc ggc cat gat att gag ttg gtg 144Ala Leu Thr Thr Thr Met Arg Thr Pro Gly His Asp Ile Glu Leu Val
35 40 45cat ggc ctc ctc ttg tca gaa ggt ctg atc acg gat gct tct gag gtt 192His Gly Leu Leu Leu Ser Glu Gly Leu Ile Thr Asp Ala Ser Glu Val
50 55 60ttt acc gcc cgc tat tgt gca gga gct gtt ggc cca gat aat caa aat 240Phe Thr Ala Arg Tyr Cys Ala Gly Ala Val Gly Pro Asp Asn Gln Asn65 70 75 80acg tac aac gtc tta gaa ctt gat gtc atc ccc aaa gac aat ccg gcc 288Thr Tyr Asn Val Leu Glu Leu Asp Val Ile Pro Lys Asp Asn Pro Ala
85 90 95cgg gat ccc gtc cag aat ccc tcc cat aat ccc gaa ggc agc caa cac 336Arg Asp Pro Val Gln Asn Pro Ser His Asn Pro Glu Gly Ser Gln His
100 105 110gaa gca ctc cac atc cca act ttc caa ccg gta cgc gaa cta aac ctc 384Glu Ala Leu His Ile Pro Thr Phe Gln Pro Val Arg Glu Leu Asn Leu
115 120 125gtg gca gcc caa cgc aat gtg ctg act acg tct gct tgt ggt gtt tgt 432Val Ala Ala Gln Arg Asn Val Leu Thr Thr Ser Ala Cys Gly Val Cys
130 135 140ggc acg acg tct att gag cag ttg atg aac aag aag ggc tgg ccc att 480Gly Thr Thr Ser Ile Glu Gln Leu Met Asn Lys Lys Gly Trp Pro Ile145 150 155 160acg ccg att aca ccg gat cct cgg atg att gtg tcg ttg cca gat aag 528Thr Pro Ile Thr Pro Asp Pro Arg Met Ile Val Ser Leu Pro Asp Lys
165 170 175ttg aag tcg aag cag aag att ttc gac aaa act ggt ggg gtt cat gct 576Leu Lys Ser Lys Gln Lys Ile Phe Asp Lys Thr Gly Gly Val His Ala
180 185 190gct ggt ttg gcc acg ctt gat ggt gag atg ttg att att cga gag gat 624Ala Gly Leu Ala Thr Leu Asp Gly Glu Met Leu Ile Ile Arg Glu Asp
195 200 205gtc ggt cgg cat aac gca gct gac aaa gtt ata gga aac atg ctg atg 672Val Gly Arg His Asn Ala Ala Asp Lys Val Ile Gly Asn Met Leu Met
210 215 220gcg gga aag ctc ccc ttg gaa aac act att ttg gtg atg agt tct agg 720Ala Gly Lys Leu Pro Leu Glu Asn Thr Ile Leu Val Met Ser Ser Arg225 230 235 240gcg tct ttt gag ctt gtc caa aag gct gcc atg gct gga att tcg ggt 768Ala Ser Phe Glu Leu Val Gln Lys Ala Ala Met Ala Gly Ile Ser Gly
245 250 255gta atc gct gtt ggt gct gca aca tcg ctg gca atc gag gcg gcg cag 816Val Ile Ala Val Gly Ala Ala Thr Ser Leu Ala Ile Glu Ala Ala Gln
260 265 270gat tca ggt att ttc ctt gct ggt ttt gtt cgg ggc aac aag ttt aac 864Asp Ser Gly Ile Phe Leu Ala Gly Phe Val Arg Gly Asn Lys Phe Asn
275 280 285cac tat gcg ggc gag ctc gga taatgccaga acaggtagaa cag 908His Tyr Ala Gly Glu Leu Gly
290 295<210>726<211>295<212>PRT<213>谷氨酸棒杆菌<400>726Pro Arg Val Val Ser Thr Asp Glu Gln Val Phe Val Asn Thr Arg Pro1 5 10 15Asp Thr Val Ala Val Glu Glu Pro Leu Glu Ile Arg Val Asn Gly Thr
20 25 30Ala Leu Thr Thr Thr Met Arg Thr Pro Gly His Asp Ile Glu Leu Val
35 40 45His Gly Leu Leu Leu Ser Glu Gly Leu Ile Thr Asp Ala Ser Glu Val
50 55 60Phe Thr Ala Arg Tyr Cys Ala Gly Ala Val Gly Pro Asp Asn Gln Asn65 70 75 80Thr Tyr Asn Val Leu Glu Leu Asp Val Ile Pro Lys Asp Asn Pro Ala
85 90 95Arg Asp Pro Val Gln Asn Pro Ser His Asn Pro Glu Gly Ser Gln His
100 105 110Glu Ala Leu His Ile Pro Thr Phe Gln Pro Val Arg Glu Leu Asn Leu
115 120 125Val Ala Ala Gln Arg Asn Val Leu Thr Thr Ser Ala Cys Gly Val Cys
130 135 140Gly Thr Thr Ser Ile Glu Gln Leu Met Asn Lys Lys Gly Trp Pro Ile145 150 155 160Thr Pro Ile Thr Pro Asp Pro Arg Met Ile Val Ser Leu Pro Asp Lys
165 170 175Leu Lys Ser Lys Gln Lys Ile Phe Asp Lys Thr Gly Gly Val His Ala
180 185 190Ala Gly Leu Ala Thr Leu Asp Gly Glu Met Leu Ile Ile Arg Glu Asp
195 200 205Val Gly Arg His Asn Ala Ala Asp Lys Val Ile Gly Asn Met Leu Met
210 215 220Ala Gly Lys Leu Pro Leu Glu Asn Thr Ile Leu Val Met Ser Ser Arg225 230 235 240Ala Ser Phe Glu Leu Val Gln Lys Ala Ala Met Ala Gly Ile Ser Gly
245 250 255Val Ile Ala Val Gly Ala Ala Thr Ser Leu Ala Ile Glu Ala Ala Gln
260 265 270Asp Ser Gly Ile Phe Leu Ala Gly Phe Val Arg Gly Asn Lys Phe Asn
275 280 285His Tyr Ala Gly Glu Leu Gly
290 295<210>727<211>1134<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1111)<223>RXN00388<400>727gaagagtact tcgaccacga cgactaacac cgcaatttaa aggcttttca agcctgcccc 60acatcgaagc agttttcaca aagaataagg ttggaaaatt atg ttg ccc gtc aac 115
Met Leu Pro Val Asn
1 5caa acg tat gcg cag ttc tca gac act gcc ttc gta tcg gca tac atc 163Gin Thr Tyr Ala Gln Phe Ser Asp Thr Ala Phe Val Ser Ala Tyr Ile
10 15 20atc tac gtt ctg gca ctc atc ctc tcc ctc gtc tac tac gta aaa caa 211Ile Tyr Val Leu Ala Leu Ile Leu Ser Leu Val Tyr Tyr Val Lys Gln
25 30 35caa ggc att atc gac gcc cgc cgc gag caa acc cgc gtc agc gaa ctc 259Gln Gly Ile Ile Asp Ala Arg Arg Glu Gln Thr Arg Val Ser Glu Leu
40 45 50gtt ggt gca ggc ggc agc gct gat gtt gat act gac ctg cct gat gac 307Val Gly Ala Gly Gly Ser Ala Asp Val Asp Thr Asp Leu Pro Asp Asp
55 60 65atc gcc gac ggt gtc ctc gcc gac gaa gac ctt gca aaa cgc gaa gaa 355Ile Ala Asp Gly Val Leu Ala Asp Glu Asp Leu Ala Lys Arg Glu Glu70 75 80 85acc gca cgc aaa cta gcc aac atg acc caa tct ctc atg tgg ctc ggc 403Thr Ala Arg Lys Leu Ala Asn Met Thr Gln Ser Leu Met Trp Leu Gly
90 95 100gtc atg gtg cac ctc gta tcc gtc gtg atg cgc gcg ctg tct gcc agc 451Val Met Val His Leu Val Ser Val Val Met Arg Ala Leu Ser Ala Ser
105 110 115cga ttc ccc ttc ggc aac ctg tat gaa tac atc ctc atg gtc acc ctc 499Arg Phe Pro Phe Gly Asn Leu Tyr Glu Tyr Ile Leu Met Val Thr Leu
120 125 130ttc gcc atg atc gga gcc gta ctc atc ctg cag cgc cca caa ttc cgc 547Phe Ala Met Ile Gly Ala Val Leu Ile Leu Gln Arg Pro Gln Phe Arg
135 140 145gtg gta tgg cca tgg atc ctc acc cca atg ctg gca ctg ctc ttc tac 595Val Val Trp Pro Trp Ile Leu Thr Pro Met Leu Ala Leu Leu Phe Tyr150 155 160 165ggt ggc acc cag ctg tac tcc gac gca gca cca gtc gtt cca gca ctg 643Gly Gly Thr Gln Leu Tyr Ser Asp Ala Ala Pro Val Val Pro Ala Leu
170 175 180cag tcc ttc tgg ttc ccg atc cac gtt tcc tcc gtc tcc atc ggc gca 691Gln Ser Phe Trp Phe Pro Ile His Val Ser Ser Val Ser Ile Gly Ala
185 190 195tcc atc ggt atc gtc tcc ggt att gca tcc ctg ctg tac ata ctg cgc 739Ser Ile Gly Ile Val Ser Gly Ile Ala Ser Leu Leu Tyr Ile Leu Arg
200 205 210atg tgg caa cca aag ggt aaa gaa aag ggc ttc ttc ggc gca gta gca 787Met Trp Gln Pro Lys Gly Lys Glu Lys Gly Phe Phe Gly Ala Val Ala
215 220 225aaa cca ctc cca tcc gga aaa acc ctg gat aac ctg gca tac aag acc 835Lys Pro Leu Pro Ser Gly Lys Thr Leu Asp Asn Leu Ala Tyr Lys Thr230 235 240 245gcg atc tgg act gtc cca atc ttc ggc ctg ggc atc atc ttg ggt gcc 883Ala Ile Trp Thr Val Pro Ile Phe Gly Leu Gly Ile Ile Leu Gly Ala
250 255 260atc tgg gca gaa gca gcc tgg ggt cgt ttc tgg gga tgg gat cct aag 931Ile Trp Ala Glu Ala Ala Trp Gly Arg Phe Trp Gly Trp Asp Pro Lys
265 270 275gaa aca gtc tcc ttc atc acc tgg gtt ctc tac gct ggt tac ctc cac 979Glu Thr Val Ser Phe Ile Thr Trp Val Leu Tyr Ala Gly Tyr Leu His
280 285 290gca cgt gca act gct ggt tgg cgc aac acc aac gct gca tgg atc aac 1027Ala Arg Ala Thr Ala Gly Trp Arg Asn Thr Asn Ala Ala Trp Ile Asn
295 300 305atc ctg gcg ctg gtc acg atg att ttt aat ctg ttc ttc atc aac atg 1075Ile Leu Ala Leu Val Thr Met Ile Phe Asn Leu Phe Phe Ile Asn Met310 315 320 325gtc gta tct ggt ctg cac tct tac gcc gga ctg aac taagcacttt 1121Val Val Ser Gly Leu His Ser Tyr Ala Gly Leu Asn
330 335tggttggcgg ggt 1134<210>728<211>337<212>PRT<213>谷氨酸棒杆菌<400>728Met Leu Pro Val Asn Gln Thr Tyr Ala Gln Phe Ser Asp Thr Ala Phe1 5 10 15Val Ser Ala Tyr Ile Ile Tyr Val Leu Ala Leu Ile Leu Ser Leu Val
20 25 30Tyr Tyr Val Lys Gln Gln Gly Ile Ile Asp Ala Arg Arg Glu Gln Thr
35 40 45Arg Val Ser Glu Leu Val Gly Ala Gly Gly Ser Ala Asp Val Asp Thr
50 55 60Asp Leu Pro Asp Asp Ile Ala Asp Gly Val Leu Ala Asp Glu Asp Leu65 70 75 80Ala Lys Arg Glu Glu Thr Ala Arg Lys Leu Ala Asn Met Thr Gln Ser
85 90 95Leu Met Trp Leu Gly Val Met Val His Leu Val Ser Val Val Met Arg
100 105 110Ala Leu Ser Ala Ser Arg Phe Pro Phe Gly Asn Leu Tyr Glu Tyr Ile
115 120 125Leu Met Val Thr Leu Phe Ala Met Ile Gly Ala Val Leu Ile Leu Gln
130 135 140Arg Pro Gln Phe Arg Val Val Trp Pro Trp Ile Leu Thr Pro Met Leu145 150 155 160Ala Leu Leu Phe Tyr Gly Gly Thr Gln Leu Tyr Ser Asp Ala Ala Pro
165 170 175Val Val Pro Ala Leu Gln Ser Phe Trp Phe Pro Ile His Val Ser Ser
180 185 190Val Ser Ile Gly Ala Ser Ile Gly Ile Val Ser Gly Ile Ala Ser Leu
195 200 205Leu Tyr Ile Leu Arg Met Trp Gln Pro Lys Gly Lys Glu Lys Gly Phe
210 215 220Phe Gly Ala Val Ala Lys Pro Leu Pro Ser Gly Lys Thr Leu Asp Asn225 230 235 240Leu Ala Tyr Lys Thr Ala Ile Trp Thr Val Pro Ile Phe Gly Leu Gly
245 250 255Ile Ile Leu Gly Ala Ile Trp Ala Glu Ala Ala Trp Gly Arg Phe Trp
260 265 270Gly Trp Asp Pro Lys Glu Thr Val Ser Phe Ile Thr Trp Val Leu Tyr
275 280 285Ala Gly Tyr Leu His Ala Arg Ala Thr Ala Gly Trp Arg Asn Thr Asn
290 295 300Ala Ala Trp Ile Asn Ile Leu Ala Leu Val Thr Met Ile Phe Asn Leu305 310 315 320Phe Phe Ile Asn Met Val Val Ser G1y Leu His Ser Tyr Ala Gly Leu
325 330 335Asn<210>729<211>326<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(303)<223>FRXA00388<400>729acc ctg gat aac ctg gca tac aag acc gcg atc tgg act gtc cca atc 48Thr Leu Asp Asn Leu Ala Tyr Lys Thr Ala Ile Trp Thr Val Pro Ile1 5 10 15ttc ggc ctg ggc atc atc ttg ggt gcc atc tgg gca gaa gca gcc tgg 96Phe Gly Leu Gly Ile Ile Leu Gly Ala Ile Trp Ala Glu Ala Ala Trp
20 25 30ggt cgt ttc tgg gga tgg gat cct aag gaa aca gtc tcc ttc atc acc 144Gly Arg Phe Trp Gly Trp Asp Pro Lys Glu Thr Val Ser Phe Ile Thr
35 40 45tgg gtt ctc tac gct ggt tac ctc cac gca cgt gca act gct ggt tgg 192Trp Val Leu Tyr Ala Gly Tyr Leu His Ala Arg Ala Thr Ala Gly Trp
50 55 60cgc aac acc aac gct gca tgg atc aac atc ctg gcg ctg gtc acg atg 240Arg Asn Thr Asn Ala Ala Trp Ile Asn Ile Leu Ala Leu Val Thr Met65 70 75 80att ttt aat ctg ttc ttc atc aac atg gtc gta tct ggt ctg cac tct 288Ile Phe Asn Leu Phe Phe Ile Asn Met Val Val Ser Gly Leu His Ser
85 90 95tac gcc gga ctg aac taagcacttt tggttggcgg ggt 326Tyr Ala Gly Leu Asn
100<210>730<211>101<212>PRT<213>谷氨酸棒杆菌<400>730Thr Leu Asp Asn Leu Ala Tyr Lys Thr Ala Ile Trp Thr Val Pro Ile1 5 10 15Phe Gly Leu Gly Ile Ile Leu Gly Ala Ile Trp Ala Glu Ala Ala Trp
20 25 30Gly Arg Phe Trp Gly Trp Asp Pro Lys Glu Thr Val Ser Phe Ile Thr
35 40 45Trp Val Leu Tyr Ala Gly Tyr Leu His Ala Arg Ala Thr Ala Gly Trp
50 55 60Arg Asn Thr Asn Ala Ala Trp Ile Asn Ile Leu Ala Leu Val Thr Met65 70 75 80Ile Phe Asn Leu Phe Phe Ile Asn Met Val Val Ser Gly Leu His Ser
85 90 95Tyr Ala Gly Leu Asn
100<210>731<211>610<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(610)<223>FRXA00386<400>731gaagagtact tcgaccacga cgactaacac cgcaatttaa aggcttttca agcctgcccc 60acatcgaagc agttttcaca aagaataagg ttggaaaatt atg ttg ccc gtc aac 115
Met Leu Pro Val Asn
1 5caa acg tat gcg cag ttc tca gac act gcc ttc gta tcg gca tac atc 163Gln Thr Tyr Ala Gln Phe Ser Asp Thr Ala Phe Val Ser Ala Tyr Ile
10 15 20atc tac gtt ctg gca ctc atc ctc tcc ctc gtc tac tac gta aaa caa 211Ile Tyr Val Leu Ala Leu Ile Leu Ser Leu Val Tyr Tyr Val Lys Gln
25 30 35caa ggc att atc gac gcc cgc cgc gag caa acc cgc gtc agc gaa ctc 259Gln Gly Ile Ile Asp Ala Arg Arg Glu Gln Thr Arg Val Ser Glu Leu
40 45 50gtt ggt gca ggc ggc agc gct gat gtt gat act gac ctg cct gat gac 307Val Gly Ala Gly Gly Ser Ala Asp Val Asp Thr Asp Leu Pro Asp Asp
55 60 65atc gcc gac ggt gtc ctc gcc gac gaa gac ctt gca aaa cgc gaa gaa 355Ile Ala Asp Gly Val Leu Ala Asp Glu Asp Leu Ala Lys Arg Glu Glu70 75 80 85acc gca cgc aaa cta gcc aac atg acc caa tct ctc atg tgg ctc ggc 403Thr Ala Arg Lys Leu Ala Asn Met Thr Gln Ser Leu Met Trp Leu Gly
90 95 100gtc atg gtg cac ctc gta tcc gtc gtg atg cgc gcg ctg tct gcc agc 451Val Met Val His Leu Val Ser Val Val Met Arg Ala Leu Ser Ala Ser
105 110 115cga ttc ccc ttc ggc aac ctg tat gaa tac atc ctc atg gtc acc ctc 499Arg Phe Pro Phe Gly Asn Leu Tyr Glu Tyr Ile Leu Met Val Thr Leu
120 125 130ttc gcc atg atc gga gcc gta ctc atc ctg cag cgc cca caa ttc cgc 547Phe Ala Met Ile Gly Ala Val Leu Ile Leu Gln Arg Pro Gln Phe Arg
135 140 145gtg gta tgg cca tgg atc ctc acc cca atg gct ggc act tgg ttt tct 595Val Val Trp Pro Trp Ile Leu Thr Pro Met Ala Gly Thr Trp Phe Ser150 155 160 165acg gtg gca ccc agc 610Thr Val Ala Pro Ser
170<210>732<211>170<212>PRT<213>谷氨酸棒杆菌<400>732Met Leu Pro Val Asn Gln Thr Tyr Ala Gln Phe Ser Asp Thr Ala Phe1 5 10 15Val Ser Ala Tyr Ile Ile Tyr Val Leu Ala Leu Ile Leu Ser Leu Val
20 25 30Tyr Tyr Val Lys Gln Gln Gly Ile Ile Asp Ala Arg Arg Glu Gln Thr
35 40 45Arg Val Ser Glu Leu Val Gly Ala Gly Gly Ser Ala Asp Val Asp Thr
50 55 60Asp Leu Pro Asp Asp Ile Ala Asp Gly Val Leu Ala Asp Glu Asp Leu65 70 75 80Ala Lys Arg Glu Glu Thr Ala Arg Lys Leu Ala Asn Met Thr Gln Ser
85 90 95Leu Met Trp Leu Gly Val Met Val His Leu Val Ser Val Val Met Arg
100 105 110Ala Leu Ser Ala Ser Arg Phe Pro Phe Gly Asn Leu Tyr Glu Tyr Ile
115 120 125Leu Met Val Thr Leu Phe Ala Met Ile Gly Ala Val Leu Ile Leu Gln
130 135 140Arg Pro Gln Phe Arg Val Val Trp Pro Trp Ile Leu Thr Pro Met Ala145 150 155 160Gly Thr Trp Phe Ser Thr Val Ala Pro Ser
165 170<210>733<211>1095<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1072)<223>RXA00945<400>733tcacagtacc acccacaagc cacaaggagg gtatggaggt gggcgtctaa agccaaattt 60ttcccggtgt ttgaggcgat tgcaccgtac actaatgtgc atg ctt gaa cgc ctc 115
Met Leu Glu Arg Leu
1 5aaa cgc cta gat ccg ctc att gtc ctc att gtg ctg gct gtc att gtg 163Lys Arg Leu Asp Pro Leu Ile Val Leu Ile Val Leu Ala Val Ile Val
10 15 20gcg atc atc att cca gtt cgc ggg gtt gct gcg gat tgg ttt gat gtc 211Ala Ile Ile Ile Pro Val Arg Gly Val Ala Ala Asp Trp Phe Asp Val
25 30 35gcc gtc aag att gcc att gcg ctg ctg ttt ttt ctt tat ggt gcc cgc 259Ala Val Lys Ile Ala Ile Ala Leu Leu Phe Phe Leu Tyr Gly Ala Arg
40 45 50cta tcc acc caa gag gcg ctg aat ggt ctg aag cac tgg agg ctt cac 307Leu Ser Thr Gln Glu Ala Leu Asn Gly Leu Lys His Trp Arg Leu His
55 60 65ctg act att ttg gcg atc act ttc gga ata ttc cca ctt atc ggc att 355Leu Thr Ile Leu Ala Ile Thr Phe Gly Ile Phe Pro Leu Ile Gly Ile70 75 80 85ggg ctc gag ccg atg act gca ttt gtg tcg gaa gat att tat cgg gga 403Gly Leu Glu Pro Met Thr Ala Phe Val Ser Glu Asp Ile Tyr Arg Gly
90 95 100att ttg ttc ctc acg ctc gtt ccg tcc acc gtg cag tca tcg gtg gcg 451Ile Leu Phe Leu Thr Leu Val Pro Ser Thr Val Gln Ser Ser Val Ala
105 110 115ttt acc tcg atc gct aaa ggc aac gta gct ggt gcg att gtg tcg gca 499Phe Thr Ser Ile Ala Lys Gly Asn Val Ala Gly Ala Ile Val Ser Ala
120 125 130tcg ctc tcc aac ctt gcg ggt gtt ttc ctc act ccg ctg ctg gtc atg 547Ser Leu Ser Asn Leu Ala Gly Val Phe Leu Thr Pro Leu Leu Val Met
135 140 145ctc atc atg tct gcg ggc ggg gga gtt cac gtg gat tcc cag gtc ttc 595Leu Ile Met Ser Ala Gly Gly Gly Val His Val Asp Ser Gln Val Phe150 155 160 165ctc gac att gcg att cag ctt ctg ctg ccg ttc atc ctc ggc cag gta 643Leu Asp Ile Ala Ile Gln Leu Leu Leu Pro Phe Ile Leu Gly Gln Val
170 175 180tgt agg cgt tgg gtg aag aat ttt gcg gcc aac aaa gca aca aaa atc 691Cys Arg Arg Trp Val Lys Asn Phe Ala Ala Asn Lys Ala Thr Lys Ile
185 190 195gtg gac cgc ggc tcg atc gcg atg gtc gtg tac tcc gcg ttt tct gcc 739Val Asp Arg Gly Ser Ile Ala Met Val Val Tyr Ser Ala Phe Ser Ala
200 205 210ggc atg gtg gct ggc att tgg tcc act gtg agc gtt cta gag att atc 787Gly Met Val Ala Gly Ile Trp Ser Thr Val Ser Val Leu Glu Ile Ile
215 220 225tac ctc att gtt ttc gct att ctg ctg gtg atg gcc atg ctg tgg ttc 835Tyr Leu Ile Val Phe Ala Ile Leu Leu Val Met Ala Met Leu Trp Phe230 235 240 245acg ctg ttc atg gct aca cgc ctt gga ttt aac cgg gca gat tcc atc 883Thr Leu Phe Met Ala Thr Arg Leu Gly Phe Asn Arg Ala Asp Ser Ile
250 255 260gct att cag ttc tgc gga acc aag aaa tcc ctg gcc aca ggc ctc cca 931Ala Ile Gln Phe Cys Gly Thr Lys Lys Ser Leu Ala Thr Gly Leu Pro
265 270 275atg gcg gca gtc atc ttc ggt ggc gcc aat atc ggc ctg ctc atc ttg 979Met Ala Ala Val Ile Phe Gly Gly Ala Asn Ile Gly Leu Leu Ile Leu
280 285 290ccg ttg atg atc ttc cac caa gtc cag ctg atg att tgt gca tgg ctt 1027Pro Leu Met Ile Phe His Gln Val Gln Leu Met Ile Cys Ala Trp Leu
295 300 305gca gct cgt tat ggt cgt gat gcg cag gaa cag aaa gcc aac gcc 1072Ala Ala Arg Tyr Gly Arg Asp Ala Gln Glu Gln Lys Ala Asn Ala310 315 320taaaagtcct cagtagctag cca 1095<210>734<211>324<212>PRT<213>谷氨酸棒杆菌<400>734Met Leu Glu Arg Leu Lys Arg Leu Asp Pro Leu Ile Val Leu Ile Val1 5 10 15Leu Ala Val Ile Val Ala Ile Ile Ile Pro Val Arg Gly Val Ala Ala
20 25 30Asp Trp Phe Asp Val Ala Val Lys Ile Ala Ile Ala Leu Leu Phe Phe
35 40 45Leu Tyr Gly Ala Arg Leu Ser Thr Gln Glu Ala Leu Asn Gly Leu Lys
50 55 60His Trp Arg Leu His Leu Thr Ile Leu Ala Ile Thr Phe Gly Ile Phe65 70 75 80Pro Leu Ile Gly Ile Gly Leu Glu Pro Met Thr Ala Phe Val Ser Glu
85 90 95Asp Ile Tyr Arg Gly Ile Leu Phe Leu Thr Leu Val Pro Ser Thr Val
100 105 110Gln Ser Ser Val Ala Phe Thr Ser Ile Ala Lys Gly Asn Val Ala Gly
115 120 125Ala Ile Val Ser Ala Ser Leu Ser Asn Leu Ala Gly Val Phe Leu Thr
130 135 140Pro Leu Leu Val Met Leu Ile Met Ser Ala Gly Gly Gly Val His Val145 150 155 160Asp Ser Gln Val Phe Leu Asp Ile Ala Ile Gln Leu Leu Leu Pro Phe
165 170 175Ile Leu Gly Gln Val Cys Arg Arg Trp Val Lys Asn Phe Ala Ala Asn
180 185 190Lys Ala Thr Lys Ile Val Asp Arg Gly Ser Ile Ala Met Val Val Tyr
195 200 205Ser Ala Phe Ser Ala Gly Met Val Ala Gly Ile Trp Ser Thr Val Ser
210 215 220Val Leu Glu Ile Ile Tyr Leu Ile Val Phe Ala Ile Leu Leu Val Met225 230 235 240Ala Met Leu Trp Phe Thr Leu Phe Met Ala Thr Arg Leu Gly Phe Asn
245 250 255Arg Ala Asp Ser Ile Ala Ile Gln Phe Cys Gly Thr Lys Lys Ser Leu
260 265 270Ala Thr Gly Leu Pro Met Ala Ala Val Ile Phe Gly Gly Ala Asn Ile
275 280 285Gly Leu Leu Ile Leu Pro Leu Met Ile Phe His Gln Val Gln Leu Met
290 295 300Ile Cys Ala Trp Leu Ala Ala Arg Tyr Gly Arg Asp Ala Gln Glu Gln305 310 315 320Lys Ala Asn Ala<210>735<211>1281<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1258)<223>RXN02556<400>735tgccatcata ttaaggccaa attgcttgga tcctgggatt tatttaatta gattaaatcc 60gtagaaatta gcccatgaag catggaaagg cgaaaacccc ttg atc gtt tcc acc 115
Leu Ile Val Ser Thr
1 5cag ccc att act gat cgc agc gca ctc tcg gca gaa cac gca gag gtg 163Gln Pro Ile Thr Asp Arg Ser Ala Leu Ser Ala Glu His Ala Glu Val
10 15 20atc aaa gca acg ctt cct ctc gtg ggc ggc aag att aat gag atc acg 211Ile Lys Ala Thr Leu Pro Leu Val Gly Gly Lys Ile Asn Glu Ile Thr
25 30 35ccg gtt ttc tac aac aag atg ttt gcg gct cac cca gaa ttg atc gct 259Pro Val Phe Tyr Asn Lys Met Phe Ala Ala His Pro Glu Leu Ile Ala
40 45 50aac acc ttc aac cgt ggc aat cag aag caa ggc gat cag cag aag gcg 307Asn Thr Phe Asn Arg Gly Asn Gln Lys Gln Gly Asp Gln Gln Lys Ala
55 60 65ctg gcg gct tcg att gca acg ttt gcc acc atg ctc gtt act cct gat 355Leu Ala Ala Ser Ile Ala Thr Phe Ala Thr Met Leu Val Thr Pro Asp70 75 80 85gct cct gac cca gtt cag ctg ctg tcc cgc att ggc cac aag cac gtg 403Ala Pro Asp Pro Val Gln Leu Leu Ser Arg Ile Gly His Lys His Val
90 95 100tcc ctc ggc att act gct gat cag tac gac att gtt cac gag cac ctg 451Ser Leu Gly Ile Thr Ala Asp Gln Tyr Asp Ile Val His Glu His Leu
105 110 115ttc gcc gca atc gtt gag gtt ttg gga gcg gaa act gtc acc gca cct 499Phe Ala Ala Ile Val Glu Val Leu Gly Ala Glu Thr Val Thr Ala Pro
120 125 130gtc gct gaa gcc tgg gat gct gtc tac tgg atc atg gca aat gtg ctg 547Val Ala Glu Ala Trp Asp Ala Val Tyr Trp Ile Met Ala Asn Val Leu
135 140 145atc ggt ttt gag aac aac ctt tat gct tcc aac gat ctg gag cct ggc 595Ile Gly Phe Glu Asn Asn Leu Tyr Ala Ser Asn Asp Leu Glu Pro Gly150 155 160 165gac gtc ttc cgc gaa gtc acc gtg acc gcg aag aag cag ctc agc gca 643Asp Val Phe Arg Glu Val Thr Val Thr Ala Lys Lys Gln Leu Ser Ala
170 175 180acc gtc tgg gaa tac acc ctg gca ggt gag ctg gtt gcc cca gag cca 691Thr Val Trp Glu Tyr Thr Leu Ala Gly Glu Leu Val Ala Pro Glu Pro
185 190 195ggt cag tac acc tcc atc gga gta gtg ctt gac gac ggc gcc cgc cag 739Gly Gln Tyr Thr Ser Ile Gly Val Val Leu Asp Asp Gly Ala Arg Gln
200 205 210ctg cgc cag tac agc ttg ctc ggc ggc tcc gac acc gag tac cgc att 787Leu Arg Gln Tyr Ser Leu Leu Gly Gly Ser Asp Thr Glu Tyr Arg Ile
215 220 225gcg gtt gag gat aac ggc gag gtt tct gga ttc ctg cgt gat cgc gta 835Ala Val Glu Asp Asn Gly Glu Val Ser Gly Phe Leu Arg Asp Arg Val230 235 240 245tcc gtt ggt gac aag att gaa gcc acc atc gcg gcc ggc gac ctg gtt 883Ser Val Gly Asp Lys Ile Glu Ala Thr Ile Ala Ala Gly Asp Leu Val
250 255 260ctt aac aag gac acc aat cca gtt gtg ctg att tcc caG ggc atc ggc 931Leu Asn Lys Asp Thr Asn Pro Val Val Leu Ile Ser Gln Gly Ile Gly
265 270 275tcc acc cca atg gtg ggc atg ctc gca ggt atg aac cct gaa cgt gac 979Ser Thr Pro Met Val Gly Met Leu Ala Gly Met Asn Pro Glu Arg Asp
280 285 290gtt gtg gtt ttg cat gct gac cag gcc gag tcc acc tac gcg cag gtg 1027Val Val Val Leu His Ala Asp Gln Ala Glu Ser Thr Tyr Ala Gln Val
295 300 305gag gaa gtg cag ggg ctc gtc gaa aag ctc cct aag gct gcg ttt gaa 1075Glu Glu Val Gln Gly Leu Val Glu Lys Leu Pro Lys Ala Ala Phe Glu310 315 320 325atc ttc tac cgc gac aac gac cag tgg ctc gag gtc gct ggc cgc att 1123Ile Phe Tyr Arg Asp Asn Asp Gln Trp Leu Glu Val Ala Gly Arg Ile
330 335 340cca tca ggt gcg tcc gtg tac ctg tgc ggt ggc gtg gaa ttc ttg aag 1171Pro Ser Gly Ala Ser Val Tyr Leu Cys Gly Gly Val Glu Phe Leu Lys
345 350 355aac gtg cgt gag cag atc gag gcg ctc gat gag cag cct cgc gac gta 1219Asn Val Arg Glu Gln Ile Glu Ala Leu Asp Glu Gln Pro Arg Asp Val
360 365 370aac ttc gag ctc ttc gca cca aac gac tgg ctg att tcc taagcccaca 1268Asn Phe Glu Leu Phe Ala Pro Asn Asp Trp Leu Ile Ser
375 380 385ccccagaact tcc 1281<210>736<211>386<212>PRT<213>谷氨酸棒杆菌<400>736Leu Ile Val Ser Thr Gln Pro Ile Thr Asp Arg Ser Ala Leu Ser Ala1 5 10 15Glu His Ala Glu Val Ile Lys Ala Thr Leu Pro Leu Val Gly Gly Lys
20 25 30Ile Asn Glu Ile Thr Pro Val Phe Tyr Asn Lys Met Phe Ala Ala His
35 40 45Pro Glu Leu Ile Ala Asn Thr Phe Asn Arg Gly Asn Gln Lys Gln Gly
50 55 60Asp Gln Gln Lys Ala Leu Ala Ala Ser Ile Ala Thr Phe Ala Thr Met65 70 75 80Leu Val Thr Pro Asp Ala Pro Asp Pro Val Gln Leu Leu Ser Arg Ile
85 90 95Gly His Lys His Val Ser Leu Gly Ile Thr Ala Asp Gln Tyr Asp Ile
100 105 110Val His Glu His Leu Phe Ala Ala Ile Val Glu Val Leu Gly Ala Glu
115 120 125Thr Val Thr Ala Pro Val Ala Glu Ala Trp Asp Ala Val Tyr Trp Ile
130 135 140Met Ala Asn Val Leu Ile Gly Phe Glu Asn Asn Leu Tyr Ala Ser Asn145 150 155 160Asp Leu Glu Pro Gly Asp Val Phe Arg Glu Val Thr Val Thr Ala Lys
165 170 175Lys Gln Leu Ser Ala Thr Val Trp Glu Tyr Thr Leu Ala Gly Glu Leu
180 185 190Val Ala Pro Glu Pro Gly Gln Tyr Thr Ser Ile Gly Val Val Leu Asp
195 200 205Asp Gly Ala Arg Gln Leu Arg Gln Tyr Ser Leu Leu Gly Gly Ser Asp
210 215 220Thr Glu Tyr Arg Ile Ala Val Glu Asp Asn Gly Glu Val Ser Gly Phe225 230 235 240Leu Arg Asp Arg Val Ser Val Gly Asp Lys Ile Glu Ala Thr Ile Ala
245 250 255Ala Gly Asp Leu Val Leu Asn Lys Asp Thr Asn Pro Val Val Leu Ile
260 265 270Ser Gln Gly Ile Gly Ser Thr Pro Met Val Gly Met Leu Ala Gly Met
275 280 285Asn Pro Glu Arg Asp Val Val Val Leu His Ala Asp Gln Ala Glu Ser
290 295 300Thr Tyr Ala Gln Val Glu Glu Val Gln Gly Leu Val Glu Lys Leu Pro305 310 315 320Lys Ala Ala Phe Glu Ile Phe Tyr Arg Asp Asn Asp Gln Trp Leu Glu
325 330 335Val Ala Gly Arg Ile Pro Ser Gly Ala Ser Val Tyr Leu Cys Gly Gly
340 345 350Val Glu Phe Leu Lys Asn Val Arg Glu Gln Ile Glu Ala Leu Asp Glu
355 360 365Gln Pro Arg Asp Val Asn Phe Glu Leu Phe Ala Pro Asn Asp Trp Leu
370 375 380Ile Ser385<210>737<211>1281<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1258)<223>FRXA02556<400>737tgccatcata ttaaggccaa attgcttgga tcctgggatt tatttaatta gattaaatcc 60gtagaaatta gcccatgaag catggaaagg cgaaaacccc ttg atc gtt tcc acc 115
Leu Ile Val Ser Thr
1 5cag ccc att act gat cgc agc gca ctc tcg gca gaa cac gca gag gtg 163Gln Pro Ile Thr Asp Arg Ser Ala Leu Ser Ala Glu His Ala Glu Val
10 15 20atc aaa gca acg ctt cct ctc gtg ggc ggc aag att aat gag atc acg 211Ile Lys Ala Thr Leu Pro Leu Val Gly Gly Lys Ile Asn Glu Ile Thr
25 30 35ccg gtt ttc tac aac aag atg ttt gcg gct cac cca gaa ttg arc gct 259Pro Val Phe Tyr Asn Lys Met Phe Ala Ala His Pro Glu Leu Ile Ala
40 45 50aac acc ttc aac ggt ggc aat cag aag caa ggc gat cag cag aag gcg 307Asn Thr Phe Asn Gly Gly Asn Gln Lys Gln Gly Asp Gln Gln Lys Ala
55 60 65ctg gcg gct tcg att gca acg ttt gcc acc atg ctc gtt act cct gat 355Leu Ala Ala Ser Ile Ala Thr Phe Ala Thr Met Leu Val Thr Pro Asp70 75 80 85gct cct gac cca gtt cag ctg ctg tcc cgc att ggc cac aag cac gtg 403Ala Pro Asp Pro Val Gln Leu Leu Ser Arg Ile Gly His Lys His Val
90 95 100tcc ctc ggc att act gct gat cag tac gac att gtt cac gag cac ctg 451Ser Leu Gly Ile Thr Ala Asp Gln Tyr Asp Ile Val His Glu His Leu
105 110 115ttc gcc gca atc gtt gag gtt ttg gga gcg gaa act gtc acc gca cct 499Phe Ala Ala Ile Val Glu Val Leu Gly Ala Glu Thr Val Thr Ala Pro
120 125 130gtc gct gaa gcc tgg gat gct gtc tac tgg atc atg gca aat gtg ctg 547Val Ala Glu Ala Trp Asp Ala Val Tyr Trp Ile Met Ala Asn Val Leu
135 140 145atc ggt ttt gag aac aac ctt tat gct tcc aac gat ctg gag cct ggc 595Ile Gly Phe Glu Asn Asn Leu Tyr Ala Ser Asn Asp Leu Glu Pro Gly150 155 160 165gac gtc ttc cgc gaa gtc acc gtg acc gcg aag aag cag ctc agc gca 643Asp Val Phe Arg Glu Val Thr Val Thr Ala Lys Lys Gln Leu Ser Ala
170 175 180acc gtc tgg gaa tac acc ctg gca ggt gag ctg gtt gcc cca gag cca 691Thr Val Trp Glu Tyr Thr Leu Ala Gly Glu Leu Val Ala Pro Glu Pro
185 190 195ggt cag tac acc tcc atc gga gta gtg ctt gac gac ggc gcc cgc cag 739Gly Gln Tyr Thr Ser Ile Gly Val Val Leu Asp Asp Gly Ala Arg Gln
200 205 210ctg cgc cag tac agc ttg ctc ggc ggc tcc gac acc gag tac cgc att 787Leu Arg Gln Tyr Ser Leu Leu Gly Gly Ser Asp Thr Glu Tyr Arg Ile
215 220 225gcg gtt gag gat aac ggc gag gtt tct gga ttc ctg cgt gat cgc gta 835Ala Val Glu Asp Asn Gly Glu Val Ser Gly Phe Leu Arg Asp Arg Val230 235 240 245tcc gtt ggt gac aag att gaa gcc acc atc gcg gcc ggc gac ctg gtt 883Ser Val Gly Asp Lys Ile Glu Ala Thr Ile Ala Ala Gly Asp Leu Val
250 255 260ctt aac aag gac acc aat cca gtt gtg ctg att tcc cag ggc atc ggc 931Leu Asn Lys Asp Thr Asn Pro Val Val Leu Ile Ser Gln Gly Ile Gly
265 270 275tcc acc cca atg gtg ggc atg ctc gca ggt atg aac cct gaa cgt gac 979Ser Thr Pro Met Val Gly Met Leu Ala Gly Met Asn Pro Glu Arg Asp
280 285 290gtt gtg gtt ttg cat gct gac cag gcc gag tcc acc tac gcg cag gtg 1027Val Val Val Leu His Ala Asp Gln Ala Glu Ser Thr Tyr Ala Gln Val
295 300 305gag gaa gtg cag ggg ctc gtc gaa aag ctc cct aag gct gcg ttt gaa 1075Glu Glu Val Gln Gly Leu Val Glu Lys Leu Pro Lys Ala Ala Phe Glu310 315 320 325atc ttc tac cgc gac aac gac cag tgg ctc gag gtc gct ggc cgc att 1123Ile Phe Tyr Arg Asp Asn Asp Gln Trp Leu Glu Val Ala Gly Arg Ile
330 335 340cca tca ggt gcg tcc gtg tac ctg tgc ggt ggc gtg gaa ttc ttg aag 1171Pro Ser Gly Ala Ser Val Tyr Leu Cys Gly Gly Val Glu Phe Leu Lys
345 350 355aac gtg cgt gag cag atc gag gcg ctc gat gag cag cct cgc gac gta 1219Asn Val Arg Glu Gln Ile Glu Ala Leu Asp Glu Gln Pro Arg Asp Val
360 365 370aac ttc gag ctc ttc gca cca aac gac tgg ctg att tcc taagcccaca 1268Asn Phe Glu Leu Phe Ala Pro Asn Asp Trp Leu Ile Ser
375 380 385ccccagaact tcc 1281<210>738<211>386<212>PRT<213>谷氨酸棒杆菌<400>738Leu Ile Val Ser Thr Gln Pro Ile Thr Asp Arg Ser Ala Leu Ser Ala1 5 10 15Glu His Ala Glu Val Ile Lys Ala Thr Leu Pro Leu Val Gly Gly Lys
20 25 30Ile Asn Glu Ile Thr Pro Val Phe Tyr Asn Lys Met Phe Ala Ala His
35 40 45Pro Glu Leu Ile Ala Asn Thr Phe Asn Gly Gly Asn Gln Lys Gln Gly
50 55 60Asp Gln Gln Lys Ala Leu Ala Ala Ser Ile Ala Thr Phe Ala Thr Met65 70 75 80Leu Val Thr Pro Asp Ala Pro Asp Pro Val Gln Leu Leu Ser Arg Ile
85 90 95Gly His Lys His Val Ser Leu Gly Ile Thr Ala Asp Gln Tyr Asp Ile
100 105 110Val His Glu His Leu Phe Ala Ala Ile Val Glu Val Leu Gly Ala Glu
115 120 125Thr Val Thr Ala Pro Val Ala Glu Ala Trp Asp Ala Val Tyr Trp Ile
130 135 140Met Ala Asn Val Leu Ile Gly Phe Glu Asn Asn Leu Tyr Ala Ser Asn145 150 155 160Asp Leu Glu Pro Gly Asp Val Phe Arg Glu Val Thr Val Thr Ala Lys
165 170 175Lys Gln Leu Ser Ala Thr Val Trp Glu Tyr Thr Leu Ala Gly Glu Leu
180 185 190Val Ala Pro Glu Pro Gly Gln Tyr Thr Ser Ile Gly Val Val Leu Asp
195 200 205Asp Gly Ala Arg Gln Leu Arg Gln Tyr Ser Leu Leu Gly Gly Ser Asp
210 215 220Thr Glu Tyr Arg Ile Ala Val Glu Asp Asn Gly Glu Val Ser Gly Phe225 230 235 240Leu Arg Asp Arg Val Ser Val Gly Asp Lys Ile Glu Ala Thr Ile Ala
245 250 255Ala Gly Asp Leu Val Leu Asn Lys Asp Thr Asn Pro Val Val Leu Ile
260 265 270Ser Gln Gly Ile Gly Ser Thr Pro Met Val Gly Met Leu Ala Gly Met
275 280 285Asn Pro Glu Arg Asp Val Val Val Leu His Ala Asp Gln Ala Glu Ser
290 295 300Thr Tyr Ala Gln Val Glu Glu Val Gln Gly Leu Val Glu Lys Leu Pro305 310 315 320Lys Ala Ala Phe Glu Ile Phe Tyr Arg Asp Asn Asp Gln Trp Leu Glu
325 330 335Val Ala Gly Arg Ile Pro Ser Gly Ala Ser Val Tyr Leu Cys Gly Gly
340 345 350Val Glu Phe Leu Lys Asn Val Arg Glu Gln Ile Glu Ala Leu Asp Glu
355 360 365Gln Pro Arg Asp Val Asn Phe Glu Leu Phe Ala Pro Asn Asp Trp Leu
370 375 380Ile Ser385<210>739<211>1200<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1177)<223>RXA01392<400>739gtctgcaatc accccgaaca tttgttcaat cgttgatttt cattccactt cgtaatattg 60ttgacatatc atctaaattt ccaagagagg acaccacaca gtg gct aac acg tca 115
Val Ala Asn Thr Ser
1 5tcc gat tgg gca ggc gcc cca caa aat gca tca gca gac ggc gag ttc 163Ser Asp Trp Ala Gly Ala Pro Gln Asn Ala Ser Ala Asp Gly Glu Phe
10 15 20gtt cgc gat acc aac tac atc gat gac cgc atc gtc gca gac gtt cca 211Val Arg Asp Thr Asn Tyr Ile Asp Asp Arg Ile Val Ala Asp Val Pro
25 30 35gcg gga tcc gaa cca att gct cag gaa gat ggc act ttc cat tgg cct 259Ala Gly Ser Glu Pro Ile Ala Gln Glu Asp Gly Thr Phe His Trp Pro
40 45 50gtc gag gct ggt cgc tac cgt tta gtc gct gcc cgc gca tgt cca tgg 307Val Glu Ala Gly Arg Tyr Arg Leu Val Ala Ala Arg Ala Cys Pro Trp
55 60 65gca cac cgc act gtt atc acc cgt cgt ctt ctc ggc ctg gag aac gtg 355Ala His Arg Thr Val Ile Thr Arg Arg Leu Leu Gly Leu Glu Asn Val70 75 80 85atc tcg ctt ggt ctg acc ggc ccg act cac gac gtt cgt tcc tgg act 403Ile Ser Leu Gly Leu Thr Gly Pro Thr His Asp Val Arg Ser Trp Thr
90 95 100ttc gat tta gat cca aac cat ctt gat ccc gtg ctg cag att cct cgt 451Phe Asp Leu Asp Pro Asn His Leu Asp Pro Val Leu Gln Ile Pro Arg
105 110 115cta cag gac gcg tat ttc aac cgc ttc ccc gat tac ccg cgc ggc att 499Leu Gln Asp Ala Tyr Phe Asn Arg Phe Pro Asp Tyr Pro Arg Gly Ile
120 125 130act gtc cca gcg ctc gtg gag gaa tcg tct aag aag gtc gtc acc aac 547Thr Val Pro Ala Leu Val Glu Glu Ser Ser Lys Lys Val Val Thr Asn
135 140 145gat tac cct tcc atc acc atc gat ttc aat ctt gag tgg aag cag ttc 595Asp Tyr Pro Ser Ile Thr Ile Asp Phe Asn Leu Glu Trp Lys Gln Phe150 155 160 165cac cgt gag ggt gcg cct aac ctc tac ccc gcg gag ctg cgc gag gag 643His Arg Glu Gly Ala Pro Asn Leu Tyr Pro Ala Glu Leu Arg Glu Glu
170 175 180atg gcg ccg gtg atg aag cgc atc ttc act Gag gtc aac aac ggc gta 691Met Ala Pro Val Met Lys Arg Ile Phe Thr Glu Val Asn Asn Gly Val
185 190 195tac agg acc ggc ttt gcc ggt agc cag gaa gcg cac aac gag gcg tac 739Tyr Arg Thr Gly Phe Ala Gly Ser Gln Glu Ala His Asn Glu Ala Tyr
200 205 210aag cgg ctt tgg gtt gcg ttg gac tgg cta gaa gat cgc tta tcg acg 787Lys Arg Leu Trp Val Ala Leu Asp Trp Leu Glu Asp Arg Leu Ser Thr
215 220 225cga cgt tac ctc atg ggg gat cac atc acc gag gcg gat atc cgc ctc 835Arg Arg Tyr Leu Met Gly Asp His Ile Thr Glu Ala Asp Ile Arg Leu230 235 240 245tac cca acc ctc gtg cgt ttc gat gcc gtc tac cac gga cac ttc aag 883Tyr Pro Thr Leu Val Arg Phe Asp Ala Val Tyr His Gly His Phe Lys
250 255 260tgt ggc cgc aac aag atc acc gaa atg ccg aat cta tgg ggc tac ctg 931Cys Gly Arg Asn Lys Ile Thr Glu Met Pro Asn Leu Trp Gly Tyr Leu
265 270 275cgg gat ctt ttc cag acc cca ggc ttt ggc gac acc acc gat ttc acc 979Arg Asp Leu Phe Gln Thr Pro Gly Phe Gly Asp Thr Thr Asp Phe Thr
280 285 290gaa atc aag cag cac tac tac atc acc cac gcg gag att aac ccc acc 1027Glu Ile Lys Gln His Tyr Tyr Ile Thr His Ala Glu Ile Asn Pro Thr
295 300 305cgg atc gtt cca gtc gga cca gat ctg tct ggt ttc gcg aca cca cac 1075Arg Ile Val Pro Val Gly Pro Asp Leu Ser Gly Phe Ala Thr Pro His310 315 320 325ggc cgt gaa aag ctc ggc gga tcc cca ttt gct gaa ggt gtt act ctg 1123Gly Arg Glu Lys Leu Gly Gly Ser Pro Phe Ala Glu Gly Val Thr Leu
330 335 340cct ggc cca att cct gcg ggc gaa gaa gtg aaa aac cct gaa cct ttt 1171Pro Gly Pro Ile Pro Ala Gly Glu Glu Val Lys Asn Pro Glu Pro Phe
345 350 355cag aag taactaaggc cgcaatccct cga 1200Gln Lys<210>740<211>359<212>PRT<213>谷氨酸棒杆菌<400>740Val Ala Asn Thr Ser Ser Asp Trp Ala Gly Ala Pro Gln Asn Ala Ser1 5 10 15Ala Asp Gly Glu Phe Val Arg Asp Thr Asn Tyr Ile Asp Asp Arg Ile
20 25 30Val Ala Asp Val Pro Ala Gly Ser Glu Pro Ile Ala Gln Glu Asp Gly
35 40 45Thr Phe His Trp Pro Val Glu Ala Gly Arg Tyr Arg Leu Val Ala Ala
50 55 60Arg Ala Cys Pro Trp Ala His Arg Thr Val Ile Thr Arg Arg Leu Leu65 70 75 80Gly Leu Glu Asn Val Ile Ser Leu Gly Leu Thr Gly Pro Thr His Asp
85 90 95Val Arg Ser Trp Thr Phe Asp Leu Asp Pro Asn His Leu Asp Pro Val
100 105 110Leu Gln Ile Pro Arg Leu Gln Asp Ala Tyr Phe Asn Arg Phe Pro Asp
115 120 125Tyr Pro Arg Gly Ile Thr Val Pro Ala Leu Val Glu Glu Ser Ser Lys
130 135 140Lys Val Val Thr Asn Asp Tyr Pro Ser Ile Thr Ile Asp Phe Asn Leu145 150 155 160Glu Trp Lys Gln Phe His Arg Glu Gly Ala Pro Asn Leu Tyr Pro Ala
165 170 175Glu Leu Arg Glu Glu Met Ala Pro Val Met Lys Arg Ile Phe Thr Glu
180 185 190Val Asn Asn Gly Val Tyr Arg Thr Gly Phe Ala Gly Ser Gln Glu Ala
195 200 205His Asn Glu Ala Tyr Lys Arg Leu Trp Val Ala Leu Asp Trp Leu Glu
210 215 220Asp Arg Leu Ser Thr Arg Arg Tyr Leu Met Gly Asp His Ile Thr Glu225 230 235 240Ala Asp Ile Arg Leu Tyr Pro Thr Leu Val Arg Phe Asp Ala Val Tyr
245 250 255His Gly His Phe Lys Cys Gly Arg Asn Lys Ile Thr Glu Met Pro Asn
260 265 270Leu Trp Gly Tyr Leu Arg Asp Leu Phe Gln Thr Pro Gly Phe Gly Asp
275 280 285Thr Thr Asp Phe Thr Glu Ile Lys Gln His Tyr Tyr Ile Thr His Ala
290 295 300Glu Ile Asn Pro Thr Arg Ile Val Pro Val Gly Pro Asp Leu Ser Gly305 310 315 320Phe Ala Thr Pro His Gly Arg Glu Lys Leu Gly Gly Ser Pro Phe Ala
325 330 335Glu Gly Val Thr Leu Pro Gly Pro Ile Pro Ala Gly Glu Glu Val Lys
340 345 350Asn Pro Glu Pro Phe Gln Lys
355<210>741<211>1227<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1204)<223>RXA00800<400>741gactccgcag ggatggccta caagtacggt cacggactta atttctagat tgtaggtagt 60ctcgtgggca caactgaaat cttattgaaa aggagtgtcc atg agc act gta gtg 115
Met Ser Thr Val Val
1 5cct gga att gtc gca ctg tcc aag ggt gca ccg gta gaa aaa gta aac 163Pro Gly Ile Val Ala Leu Ser Lys Gly Ala Pro Val Glu Lys Val Asn
10 15 20gtt gtt gtc cct gat cca ggt gct aac gat gtc atc gtc aag att cag 211Val Val Val Pro Asp Pro Gly Ala Asn Asp Val Ile Val Lys Ile Gln
25 30 35gcc tgc ggt gtg tgc cac acc gac ttg gcc tac cgc gat ggc gat att 259Ala Cys Gly Val Cys His Thr Asp Leu Ala Tyr Arg Asp Gly Asp Ile
40 45 50tca gat gag ttc cct tac ctc ctc ggc cac gag gca gca ggc att gtt 307Ser Asp Glu Phe Pro Tyr Leu Leu Gly His Glu Ala Ala Gly Ile Val
55 60 65gag gag gta ggc gag tcc gtc acc cac gtt gag gtc ggc gat ttc gtc 355Glu Glu Val Gly Glu Ser Val Thr His Val Glu Val Gly Asp Phe Val70 75 80 85atc ttg aac tgg cgt gca gtg tgc ggc gag tgc cgt gca tgt aag aag 403Ile Leu Asn Trp Arg Ala Val Cys Gly Glu Cys Arg Ala Cys Lys Lys
90 95 100ggc gag cca aag tac tgc ttt aac acc cac aac gcc tct aag aag atg 451Gly Glu Pro Lys Tyr Cys Phe Asn Thr His Asn Ala Ser Lys Lys Met
105 110 115acc ctg gaa gac ggc acc gag ctg tcc cca gca ctg ggt att ggc gcg 499Thr Leu Glu Asp Gly Thr Glu Leu Ser Pro Ala Leu Gly Ile Gly Ala
120 125 130ttc ttg gaa aag acc ctg gtc cac gaa ggc cag tgc acc aag gtt aac 547Phe Leu Glu Lys Thr Leu Val His Glu Gly Gln Cys Thr Lys Val Asn
135 140 145cct gag gaa gat cca gca gca gct ggc ctt ctg ggt tgt ggc atc atg 595Pro Glu Glu Asp Pro Ala Ala Ala Gly Leu Leu Gly Cys Gly Ile Met150 155 160 165gca ggc ctt ggc gct gcg gtg aac acc ggt gat att aag cgt ggc gag 643Ala Gly Leu Gly Ala Ala Val Asn Thr Gly Asp Ile Lys Arg Gly Glu
170 175 180tcc gta gca gtc ttc ggc ctt ggt ggc gtg ggc atg gca gct att gct 691Ser Val Ala Val Phe Gly Leu Gly Gly Val Gly Met Ala Ala Ile Ala
185 190 195ggc gcc aag att gct ggc gct tcc aag atc att gct gtt gat atc gat 739Gly Ala Lys Ile Ala Gly Ala Ser Lys Ile Ile Ala Val Asp Ile Asp
200 205 210gag aag aag ctg gag tgg gcg aag gaa ttc ggc gca acc cac acc att 787Glu Lys Lys Leu Glu Trp Ala Lys Glu Phe Gly Ala Thr His Thr Ile
215 220 225aat tcc tct ggt ctt ggt ggc gaa ggt gat gcc tct gag gtc gtg gca 835Asn Ser Ser Gly Leu Gly Gly Glu Gly Asp Ala Ser Glu Val Val Ala230 235 240 245aag gtt cgt gag ctc acc gat ggt ttc ggc acc gat gtc tcc atc gat 883Lys Val Arg Glu Leu Thr Asp Gly Phe Gly Thr Asp Val Ser Ile Asp
250 255 260gcg gta ggc atc atg ccg acc tgg cag cag gcg ttt tac tcc cgt gac 931Ala Val Gly Ile Met Pro Thr Trp Gln Gln Ala Phe Tyr Ser Arg Asp
265 270 275cat gca ggc cgc atg gtg atg gtg ggc gtt cca aac ctg acg tct cgc 979His Ala Gly Arg Met Val Met Val Gly Val Pro Asn Leu Thr Ser Arg
280 285 290gta gat gtt cct gcg att gat ttt tac ggt cgc ggt gga tcc gtg cgc 1027Val Asp Val Pro Ala Ile Asp Phe Tyr Gly Arg Gly Gly Ser Val Arg
295 300 305cct gca tgg tac ggc gac tgc ctg cct gag cgt gat ttc cca act tat 1075Pro Ala Trp Tyr Gly Asp Cys Leu Pro Glu Arg Asp Phe Pro Thr Tyr310 315 320 325gtg gat ctg cac ctg cag ggt cgt ttc cca ctg gat aag ttt gtt tct 1123Val Asp Leu His Leu Gln Gly Arg Phe Pro Leu Asp Lys Phe Val Ser
330 335 340gag cgt att ggt ctt gat gat gtt gaa gag gct ttc aac acc atg aag 1171Glu Arg Ile Gly Leu Asp Asp Val Glu Glu Ala Phe Asn Thr Met Lys
345 350 355gct ggc gac gtg ctg cgt tot gtg gtg gag atc taaatggctc acgacggatt 1224Ala Gly Asp Val Leu Arg Ser Val Val Glu Ile
360 365gcg 1227<210>742<211>368<212>PRT<213>谷氨酸棒杆菌<400>742Met Ser Thr Val Val Pro Gly Ile Val Ala Leu Ser Lys Gly Ala Pro1 5 10 15Val Glu Lys Val Asn Val Val Val Pro Asp Pro Gly Ala Asn Asp Val
20 25 30Ile Val Lys Ile Gln Ala Cys Gly Val Cys His Thr Asp Leu Ala Tyr
35 40 45Arg Asp Gly Asp Ile Ser Asp Glu Phe Pro Tyr Leu Leu Gly His Glu
50 55 60Ala Ala Gly Ile Val Glu Glu Val Gly Glu Ser Val Thr His Val Glu65 70 75 80Val Gly Asp Phe Val Ile Leu Asn Trp Arg Ala Val Cys Gly Glu Cys
85 90 95Arg Ala Cys Lys Lys Gly Glu Pro Lys Tyr Cys Phe Asn Thr His Asn
100 105 110Ala Ser Lys Lys Met Thr Leu Glu Asp Gly Thr Glu Leu Ser Pro Ala
115 120 125Leu Gly Ile Gly Ala Phe Leu Glu Lys Thr Leu Val His Glu Gly Gln
130 135 140Cys Thr Lys Val Asn Pro Glu Glu Asp Pro Ala Ala Ala Gly Leu Leu145 150 155 160Gly Cys Gly Ile Met Ala Gly Leu Gly Ala Ala Val Asn Thr Gly Asp
165 170 175Ile Lys Arg Gly Glu Ser Val Ala Val Phe Gly Leu Gly Gly Val Gly
180 185 190Met Ala Ala Ile Ala Gly Ala Lys Ile Ala Gly Ala Ser Lys Ile Ile
195 200 205Ala Val Asp Ile Asp Glu Lys Lys Leu Glu Trp Ala Lys Glu Phe Gly
210 215 220Ala Thr His Thr Ile Asn Ser Ser Gly Leu Gly Gly Glu Gly Asp Ala225 230 235 240Ser Glu Val Val Ala Lys Val Arg Glu Leu Thr Asp Gly Phe Gly Thr
245 250 255Asp Val Ser Ile Asp Ala Val Gly Ile Met Pro Thr Trp Gln Gln Ala
260 265 270Phe Tyr Ser Arg Asp His Ala Gly Arg Met Val Met Val Gly Val Pro
275 280 285Asn Leu Thr Ser Arg Val Asp Val Pro Ala Ile Asp Phe Tyr Gly Arg
290 295 300Gly Gly Ser Val Arg Pro Ala Trp Tyr Gly Asp Cys Leu Pro Glu Arg305 310 315 320Asp Phe Pro Thr Tyr Val Asp Leu His Leu Gln Gly Arg Phe Pro Leu
325 330 335Asp Lys Phe Val Ser Glu Arg Ile Gly Leu Asp Asp Val Glu Glu Ala
340 345 350Phe Asn Thr Met Lys Ala Gly Asp Val Leu Arg Ser Val Val Glu Ile
355 360 365<210>743<211>1011<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(988)<223>RXA02143<400>743tcttcatcac tatttacttc attcagtagg cagtaaggaa tcctcaacgt tgttgaggtt 60ccctatgccc ttcacttcca cagtcgagat tcaaagggaa atg atg gaa acc aac 115
Met Met Glu Thr Asn
1 5ccg cag acc cca gag gga aat agc atg gct aaa ccc tct gct aag aag 163Pro Gln Thr Pro Glu Gly Asn Ser Met Ala Lys Pro Ser Ala Lys Lys
10 15 20gtc aag aat cgc cgc aag gtc cgg cgc acc gtc gca ggt gca ttg gct 211Val Lys Asn Arg Arg Lys Val Arg Arg Thr Val Ala Gly Ala Leu Ala
25 30 35ctg acc att gga ctg agc gga gca gga atc ctc gca acc gcg atc act 259Leu Thr Ile Gly Leu Ser Gly Ala Gly Ile Leu Ala Thr Ala Ile Thr
40 45 50cca gat gct caa gtt gct acc gct cag cgt gac gat cag gca ctt atc 307Pro Asp Ala Gln Val Ala Thr Ala Gln Arg Asp Asp Gln Ala Leu Ile
55 60 65tcc gag ggt aaa gac ctc tac gat gtc gcc tgc atc acc tgc cac ggc 355Ser Glu Gly Lys Asp Leu Tyr Asp Val Ala Cys Ile Thr Cys His Gly70 75 80 85gta aac ctc caa ggt gtt gag gac cgc ggt cct tcc ctc gta ggt gtt 403Val Asn Leu Gln Gly Val Glu Asp Arg Gly Pro Ser Leu Val Gly Val
90 95 100ggc gaa ggc gca gtg tacttc caa gtt cac tcc ggc cgt atg cca ata 451Gly Glu Gly Ala Val Tyr Phe Gln Val His Ser Gly Arg Met Pro Ile
105 110 115ctg cgt aac gag gct cag gct gag cgc aag gct cct cgt tac acc gag 499Leu Arg Asn Glu Ala Gln Ala Glu Arg Lys Ala Pro Arg Tyr Thr Glu
120 125 130gca cag acc ctt gcg atc gct gca tat gtt gca gct aat ggc ggt ggc 547Ala Gln Thr Leu Ala Ile Ala Ala Tyr Val Ala Ala Asn Gly Gly Gly
135 140 145cca gga ctc gtt tac aac gag gac ggc acc ctc gcc atg gag gag ctc 595Pro Gly Leu Val Tyr Asn Glu Asp Gly Thr Leu Ala Met Glu Glu Leu150 155 160 165cgt ggc gaa aac tac gac gga cag att acc tcc gcc gac gtc gct cgc 643Arg Gly Glu Asn Tyr Asp Gly Gln Ile Thr Ser Ala Asp Val Ala Arg
170 175 180ggc gga gat ctg ttc cgc ctg aac tgt gca tcc tgc cac aac ttc act 691Gly Gly Asp Leu Phe Arg Leu Asn Cys Ala Ser Cys His Asn Phe Thr
185 190 195ggt cgt ggt ggc gca ctg tcc tct ggt aag tac gca cca aac ctg gat 739Gly Arg Gly Gly Ala Leu Ser Ser Gly Lys Tyr Ala Pro Asn Leu Asp
200 205 210gct gca aac gag cag gaa atc tac cag gct atg ctt acc ggt cct cag 787Ala Ala Asn Glu Gln Glu Ile Tyr Gln Ala Met Leu Thr Gly Pro Gln
215 220 225aac atg cct aag ttc tcc gat cgt cag ctc tcc gca gat gag aag aag 835Asn Met Pro Lys Phe Ser Asp Arg Gln Leu Ser Ala Asp Glu Lys Lys230 235 240 245gac atc atc gcc ttc atc aag tcc acc aag gag act cca tca cct ggt 883Asp Ile Ile Ala Phe Ile Lys Ser Thr Lys Glu Thr Pro Ser Pro Gly
250 255 260ggt tac tca ctc ggt agc ttg ggc cca gtg gct gag ggt ctg ttc atg 931Gly Tyr Ser Leu Gly Ser Leu Gly Pro Val Ala Glu Gly Leu Phe Met
265 270 275tgg gta ttc ggc atc ttg gtc ctc gtg gcc gcc gct atg tgg att gga 979Trp Val Phe Gly Ile Leu Val Leu Val Ala Ala Ala Met Trp Ile Gly
280 285 290tca cgt tca tgagtaacaa caacgacaaa cag 1011Ser Arg Ser
295<210>744<211>296<212>PRT<213>谷氨酸棒杆菌<400>744Met Met Glu Thr Asn Pro Gln Thr Pro Glu Gly Asn Ser Met Ala Lys1 5 10 15Pro Ser Ala Lys Lys Val Lys Asn Arg Arg Lys Val Arg Arg Thr Val
20 25 30Ala Gly Ala Leu Ala Leu Thr Ile Gly Leu Ser Gly Ala Gly Ile Leu
35 40 45Ala Thr Ala Ile Thr Pro Asp Ala Gln Val Ala Thr Ala Gln Arg Asp
50 55 60Asp Gln Ala Leu Ile Ser Glu Gly Lys Asp Leu Tyr Asp Val Ala Cys65 70 75 80Ile Thr Cys His Gly Val Asn Leu Gln Gly Val Glu Asp Arg Gly Pro
85 90 95Ser Leu Val Gly Val Gly Glu Gly Ala Val Tyr Phe Gln Val His Ser
100 105 110Gly Arg Met Pro Ile Leu Arg Asn Glu Ala Gln Ala Glu Arg Lys Ala
115 120 125Pro Arg Tyr Thr Glu Ala Gln Thr Leu Ala Ile Ala Ala Tyr Val Ala
130 135 140Ala Asn Gly Gly Gly Pro Gly Leu Val Tyr Asn Glu Asp Gly Thr Leu145 150 155 160Ala Met Glu Glu Leu Arg Gly Glu Asn Tyr Asp Gly Gln Ile Thr Ser
165 170 175Ala Asp Val Ala Arg Gly Gly Asp Leu Phe Arg Leu Asn Cys Ala Ser
180 185 190Cys His Asn Phe Thr Gly Arg Gly Gly Ala Leu Ser Ser Gly Lys Tyr
195 200 205Ala Pro Asn Leu Asp Ala Ala Asn Glu Gln Glu Ile Tyr Gln Ala Met
210 215 220Leu Thr Gly Pro Gln Asn Met Pro Lys Phe Ser Asp Arg Gln Leu Ser225 230 235 240Ala Asp Glu Lys Lys Asp Ile Ile Ala Phe Ile Lys Ser Thr Lys Glu
245 250 255Thr Pro Ser Pro Gly Gly Tyr Ser Leu Gly Ser Leu Gly Pro Val Ala
260 265 270Glu Gly Leu Phe Met Trp Val Phe Gly Ile Leu Val Leu Val Ala Ala
275 280 285Ala Met Trp Ile Gly Ser Arg Ser
290 295<210>745<211>502<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(502)<223>RXN03096<400>745aagatcatgc tcatcgaagc cggcgcccga agatggcagt tggctggcat gggtccttat 60cgcaggcgccgttgtcacct cactgctcac cttgtacacc atg gtt ctg gtc tgg 115
Met Val Leu Val Trp
1 5tcc aag gcc ttc tgg cgc gac cgt aaa gac gcc ccc gat gga gca acc 163Ser Lys Ala Phe Trp Arg Asp Arg Lys Asp Ala Pro Asp Gly Ala Thr
10 15 20gca cta gca aga ccc gca cct ttg gta gat atc caa gac gaa gtc gcc 211Ala Leu Ala Arg Pro Ala Pro Leu Val Asp Ile Gln Asp Glu Val Ala
25 30 35gtt aaa gac cgc aac gat gtc gga cgg atg cct tgg ggc atg gtc ttc 259Val Lys Asp Arg Asn Asp Val Gly Arg Met Pro Trp Gly Met Val Phe
40 45 50tcc act gcc ctg ttg gtt tcc gca tcc ctt gct gta tcc gtg ctc gca 307Ser Thr Ala Leu Leu Val Ser Ala Ser Leu Ala Val Ser Val Leu Ala
55 60 65gga cca ctg tca tct att act gga cgc gcc gcc gaa tcc gca caa gat 355Gly Pro Leu Ser Ser Ile Thr Gly Arg Ala Ala Glu Ser Ala Gln Asp70 75 80 85gtc aac atc tac cgc gcc gca gta ctc ggc cca act acc tcg acc cat 403Val Asn Ile Tyr Arg Ala Ala Val Leu Gly Pro Thr Thr Ser Thr His
90 95 100cac gca cac tcg aga tgg agc gtt acg acg cca acc gcg atg aca tca 451His Ala His Ser Arg Trp Ser Val Thr Thr Pro Thr Ala Met Thr Ser
105 110 115acc acc gcg tcg aca cca atg gaa cgg agg acc aac cat gat cag tgg 499Thr Thr Ala Ser Thr Pro Met Glu Arg Arg Thr Asn His Asp Gln Trp
120 125 130att 502Ile<210>746<211>134<212>PRT<213>谷氨酸棒杆菌<400>746Met Val Leu Val Trp Ser Lys Ala Phe Trp Arg Asp Arg Lys Asp Ala1 5 10 15Pro Asp Gly Ala Thr Ala Leu Ala Arg Pro Ala Pro Leu Val Asp Ile
20 25 30Gln Asp Glu Val Ala Val Lys Asp Arg Asn Asp Val Gly Arg Met Pro
35 40 45Trp Gly Met Val Phe Ser Thr Ala Leu Leu Val Ser Ala Ser Leu Ala
50 55 60Val Ser Val Leu Ala Gly Pro Leu Ser Ser Ile Thr Gly Arg Ala Ala65 70 75 80Glu Ser Ala Gln Asp Val Asn Ile Tyr Arg Ala Ala Val Leu Gly Pro
85 90 95Thr Thr Ser Thr His His Ala His Ser Arg Trp Ser Val Thr Thr Pro
100 105 110Thr Ala Met Thr Ser Thr Thr Ala Ser Thr Pro Met Glu Arg Arg Thr
115 120 125Asn His Asp Gln Trp Ile
130<210>747<211>504<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(481)<223>RXN02036<400>747ctaaaatgga aggcaatgga tacccgccgc atgaacctcc gccaatggaa aatcctcgtt 60gccctcatct ctgctgcagt ggcggctctc ggagggtggt gtg cat att cct ttt 115
Val His Ile Pro Phe
1 5ggg cac ctc gcc gac acc gtc tcc tgg gac tgc ggg gga ggc agc tgc 163Gly His Leu Ala Asp Thr Val Ser Trp Asp Cys Gly Gly Gly Ser Cys
10 15 20gcc acc aac gat ttg gta tcc ctg ttc atg ccg gcc gcc ttc atg agt 211Ala Thr Asn Asp Leu Val Ser Leu Phe Met Pro Ala Ala Phe Met Ser
25 30 35acc ctc gcc gcc tgc gta ttt ggc gcg tgg gcc ata ggt ttg atc gct 259Thr Leu Ala Ala Cys Val Phe Gly Ala Trp Ala Ile Gly Leu Ile Ala
40 45 50ccc gca cta ttc atc gcg gtg act gcc tgg gca ttt cgc tcc ggc gtg 307Pro Ala Leu Phe Ile Ala Val Thr Ala Trp Ala Phe Arg Ser Gly Val
55 60 65cag gct gcg att gcc gac ggc tac acg tcc gcg act tcc gtc ggc ttc 355Gln Ala Ala Ile Ala Asp Gly Tyr Thr Ser Ala Thr Ser Val Gly Phe70 75 80 85gaa atg act gtc tcg ctc att ctt ttc atc atc gca ggt ctg tgc ttt 403Glu Met Thr Val Ser Leu Ile Leu Phe Ile Ile Ala Gly Leu Cys Phc
90 95 100ctg ggc tgg atc ccc atg ttc atc aac aac cgc caa gtc gcg cgc aag 451Leu Gly Trp Ile Pro Met Phe Ile Asn Asn Arg Gln Val Ala Arg Lys
105 110 115gtc cgc gag agg gct gcg ggc ttg agc aat taggctctcg cttttcgacg 501Val Arg Glu Arg Ala Ala Gly Leu Ser Asn
120 125ttt 504<210>748<211>127<212>PRT<213>谷氨酸棒杆菌<400>748Val His Ile Pro Phe Gly His Leu Ala Asp Thr Val Ser Trp Asp Cys1 5 10 15Gly Gly Gly Ser Cys Ala Thr Asn Asp Leu Val Ser Leu Phe Met Pro
20 25 30Ala Ala Phe Met Ser Thr Leu Ala Ala Cys Val Phe Gly Ala Trp Ala
35 40 45Ile Gly Leu Ile Ala Pro Ala Leu Phe Ile Ala Val Thr Ala Trp Ala
50 55 60Phc Arg Ser Gly Val Gln Ala Ala Ile Ala Asp Gly Tyr Thr Ser Ala65 70 75 80Thr Ser Val Gly Phe Glu Met Thr Val Ser Leu Ile Leu Phe Ile Ile
85 90 95Ala Gly Leu Cys Phe Leu Gly Trp Ile Pro Met Phe Ile Asn Asn Arg
100 105 110Gln Val Ala Arg Lys Val Arg Glu Arg Ala Ala Gly Leu Ser Asn
115 120 125<210>749<211>882<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(859)<223>RXN02765<400>749tctgaacttt tccgcatcga cccagatgag gtttaccccg acgacgacgc cacctgcgaa 60ttcaacccat ggccgtatcc tcgcggattt taggagataa atg tct aat caa tta 115
Met Ser Asn Gln Leu
1 5ccc gat cac gtc cgc gac gcc ttc caa gta ggt gcg gga cct gcc gaa 163Pro Asp His Val Arg Asp Ala Phe Gln Val Gly Ala Gly Pro Ala Glu
10 15 20caa ctc ggt caa gct tgg gac ttc gga ttc cgc gtc ggc aac act gtg 211Gln Leu Gly Gln Ala Trp Asp Phe Gly Phe Arg Val Gly Asn Thr Val
25 30 35ttc gcc aaa gtg acg gcg ccg gaa gtg tcg ggc tgg tcg tcg aaa acc 259Phe Ala Lys Val Thr Ala Pro Glu Val Ser Gly Trp Ser Ser Lys Thr
40 45 50cgc gaa acc ctg aaa cca gaa ggc gtg cgc gtc gta cga ccg atc cgc 307Arg Glu Thr Leu Lys Pro Glu Gly Val Arg Val Val Arg Pro Ile Arg
55 60 65tcc acc gac ggc cga ttt gtg gtt gcg ggg tgg cgc gca tcg gtg ttc 355Ser Thr Asp Gly Arg Phe Val Val Ala Gly Trp Arg Ala Ser Val Phe70 75 80 85tct acg gga acg atc agc aag cga gtc gat gag acg gtc gtt gcg ggt 403Ser Thr Gly Thr Ile Ser Lys Arg Val Asp Glu Thr Val Val Ala Gly
90 95 100ctt cgt ttg gca gat gca tta gtg gat acg cat gca ccg gaa cct gtg 451Leu Arg Leu Ala Asp Ala Leu Val Asp Thr His Ala Pro Glu Pro Val
105 110 115gac aat gtg ttt aac cgt gct gat gtg cag gcc tgg gaa gag cag ccc 499Asp Asn Val Phe Asn Arg Ala Asp Val Gln Ala Trp Glu Glu Gln Pro
120 125 130ggt cga atc ggt gaa ttg ttg gag ccg att aat cgc gtg aac cag gtt 547Gly Arg Ile Gly Glu Leu Leu Glu Pro Ile Asn Arg Val Asn Gln Val
135 140 145ggt cat gcg gat atg ttg gcg aca acg ctg tat gcg gga act cag cca 595Gly His Ala Asp Met Leu Ala Thr Thr Leu Tyr Ala Gly Thr Gln Pro150 155 160 165cct gca gtg acg gat ttg gtg cca gtg ctg cgt ccg cat ggt ttc act 643Pro Ala Val Thr Asp Leu Val Pro Val Leu Arg Pro His Gly Phe Thr
170 175 180gcg gca ttg gtg atc gtt gat ggg ttg ctg ctg ggt gcg gtt gat gag 691Ala Ala Leu Val Ile Val Asp Gly Leu Leu Leu Gly Ala Val Asp Glu
185 190 195gga att ctg cgg agg ttt tcg cat ttg ccg gaa att gag cag ctg gtt 739Gly Ile Leu Arg Arg Phe Ser His Leu Pro Glu Ile Glu Gln Leu Val
200 205 210ttg agg gca ttt ttg ttc cgt cga aac ttg cag gag ttc tct gag aac 787Leu Arg Ala Phe Leu Phe Arg Arg Asn Leu Gln Glu Phe Ser Glu Asn
215 220 225aac gat ccg aat gtt att tcg aac cta aac agg gtg gaa tcg aca ctc 835Asn Asp Pro Asn Val Ile Ser Asn Leu Asn Arg Val Glu Ser Thr Leu230 235 240 245gtg tcg tat gtt tct gac aag att tgaggtatgt cggaatacaa acc 882Val Ser Tyr Val Ser Asp Lys Ile
250<210>750<211>253<212>PRT<213>谷氨酸棒杆菌<400>750Met Ser Asn Gln Leu Pro Asp His Val Arg Asp Ala Phe Gln Val Gly1 5 10 15Ala Gly Pro Ala Glu Gln Leu Gly Gln Ala Trp Asp Phe Gly Phe Arg
20 25 30Val Gly Asn Thr Val Phe Ala Lys Val Thr Ala Pro Glu Val Ser Gly
35 40 45Trp Ser Ser Lys Thr Arg Glu Thr Leu Lys Pro Glu Gly Val Arg Val
50 55 60Val Arg Pro Ile Arg Ser Thr Asp Gly Arg Phe Val Val Ala Gly Trp65 70 75 80Arg Ala Ser Val Phe Ser Thr Gly Thr Ile Ser Lys Arg Val Asp Glu
85 90 95Thr Val Val Ala Gly Leu Arg Leu Ala Asp Ala Leu Val Asp Thr His
100 105 110Ala Pro Glu Pro Val Asp Asn Val Phe Asn Arg Ala Asp Val Gln Ala
115 120 125Trp Glu Glu Gln Pro Gly Arg Ile Gly Glu Leu Leu Glu Pro Ile Asn
130 135 140Arg Val Asn Gln Val Gly His Ala Asp Met Leu Ala Thr Thr Leu Tyr145 150 155 160Ala Gly Thr Gln Pro Pro Ala Val Thr Asp Leu Val Pro Val Leu Arg
165 170 175Pro His Gly Phe Thr Ala Ala Leu Val Ile Val Asp Gly Leu Leu Leu
180 185 190Gly Ala Val Asp Glu Gly Ile Leu Arg Arg Phe Ser His Leu Pro Glu
195 200 205Ile Glu Gln Leu Val Leu Arg Ala Phe Leu Phe Arg Arg Asn Leu Gln
210 215 220Glu Phe Ser Glu Asn Asn Asp Pro Asn Val Ile Ser Asn Leu Asn Arg225 230 235 240Val Glu Ser Thr Leu Val Ser Tyr Val Ser Asp Lys Ile
245 250<210>751<211>1059<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1036)<223>RXN02206<400>751ggcaggatct gctgctgcgg ctaggagggt tatctcttca ttcacccgat ctaccgtact 60accttatgac ctcagtagtg tggtgggcgt gaaacagcga atg gtc ggt tca agt 115
Met Val Gly Ser Ser
1 5ggt ttg cgg gta tcc agg ctc ggt ttg ggc acc tca aca tgg ggc tcg 163Gly Leu Arg Val Ser Arg Leu Gly Leu Gly Thr Ser Thr Trp Gly Ser
10 15 20ggc acc gag ctg gct gag gca ggc gat atc ttt aag gcg ttc atc aat 211Gly Thr Glu Leu Ala Glu Ala Gly Asp Ile Phe Lys Ala Phe Ile Asn
25 30 35tct ggt ggc acg ctt atc gac gtc tcc ccc aac tac acc acc ggc gtc 259Ser Gly Gly Thr Leu Ile Asp Val Ser Pro Asn Tyr Thr Thr Gly Val
40 45 50gcg gaa gaa atg ctc ggc acg atg ttg gat gcg gaa gtc tct cgt tcg 307Ala Glu Glu Met Leu Gly Thr Met Leu Asp Ala Glu Val Ser Arg Ser
55 60 65gct gtc gtc att tcc tcc agc gca ggt gtc aac ccc gct ctg ccg ctc 355Ala Val Val Ile Ser Ser Ser Ala Gly Val Asn Pro Ala Leu Pro Leu70 75 80 85ggc cga cgt gtg gat tgc tcc cgc cgc aat ttg att gcc caa tta gat 403Gly Arg Arg Val Asp Cys Ser Arg Arg Asn Leu Ile Ala Gln Leu Asp
90 95 100gtc acc ctg cgg gca tta aac act gac tat ttg gat ttg tgg tct gtg 451Val Thr Leu Arg Ala Leu Asn Thr Asp Tyr Leu Asp Leu Trp Ser Val
105 110 115ggc tat tgg gat gag ggc acc cca ccg cat gag gtg gcc gat act ttg 499Gly Tyr Trp Asp Glu Gly Thr Pro Pro His Glu Val Ala Asp Thr Leu
120 125 130gat tac gcc gtg cgc acc ggc cga gtc cga tat gcc ggt gtc cga gga 547Asp Tyr Ala Val Arg Thr Gly Arg Val Arg Tyr Ala Gly Val Arg Gly
135 140 145tat tcc ggt tgg cag tta gcg gtc acc cac gct gca tcc aat cat gca 595Tyr Ser Gly Trp Gln Leu Ala Val Thr His Ala Ala Ser Asn His Ala150 155 160 165gcg gcc tcc gcc cgc ccc gtg gtc gtt gca caa aat gaa tac agc ctg 643Ala Ala Ser Ala Arg Pro Val Val Val Ala Gln Asn Glu Tyr Ser Leu
170 175 180ctg gaa cgc cgc gca gaa caa gaa ctc ctc cct gcc acc caa cac cta 691Leu Glu Arg Arg Ala Glu Gln Glu Leu Leu Pro Ala Thr Gln His Leu
185 190 195ggt gtc gga ttc ttt gct ggc gct ccg ctg ggg caa ggc gtg ctg act 739Gly Val Gly Phe Phe Ala Gly Ala Pro Leu Gly Gln Gly Val Leu Thr
200 205 210gct aaa tac cgc tcc gaa att ccc cat gat tcc aga gct gca tcc aca 787Ala Lys Tyr Arg Ser Glu Ile Pro His Asp Ser Arg Ala Ala Ser Thr
215 220 225gga cgc gac gca gaa gtc caa agc tac cta gat aat cga ggc cgc atc 835Gly Arg Asp Ala Glu Val Gln Ser Tyr Leu Asp Asn Arg Gly Arg Ile230 235 240 245att gtc gat gct ctt gat act gca gcc aaa gga tta ggc att agc ccc 883Ile Val Asp Ala Leu Asp Thr Ala Ala Lys Gly Leu Gly Ile Ser Pro
250 255 260gct gtc aca gcc acc acc tgg gtg cgt gat cgt ccc gga gtg aca gct 931Ala Val Thr Ala Thr Thr Trp Val Arg Asp Arg Pro Gly Val Thr Ala
265 270 275gtc atc gtg ggc gct cgc aca cat gaa cag ctg tca cat ctt ctc aag 979Val Ile Val Gly Ala Arg Thr His Glu Gln Leu Ser His Leu Leu Lys
280 285 290gcg gaa tcg gtg act ttg cca aca cca atc aca caa gcc ctt gat gat 1027Ala Glu Ser Val Thr Leu Pro Thr Pro Ile Thr Gln Ala Leu Asp Asp
295 300 305gtc tcc ctg tgacttggtc caattacatt cac 1059Val Ser Leu310<210>752<211>312<212>PRT<213>谷氨酸棒杆菌<400>752Met Val Gly Ser Ser Gly Leu Arg Val Ser Arg Leu Gly Leu Gly Thr1 5 10 15Ser Thr Trp Gly Ser Gly Thr Glu Leu Ala Glu Ala Gly Asp Ile Phe
20 25 30Lys Ala Phe Ile Asn Ser Gly Gly Thr Leu Ile Asp Val Ser Pro Asn
35 40 45Tyr Thr Thr Gly Val Ala Glu Glu Met Leu Gly Thr Met Leu Asp Ala
50 55 60Glu Val Ser Arg Ser Ala Val Val Ile Ser Ser Ser Ala Gly Val Asn65 70 75 80Pro Ala Leu Pro Leu Gly Arg Arg Val Asp Cys Ser Arg Arg Asn Leu
85 90 95Ile Ala Gln Leu Asp Val Thr Leu Arg Ala Leu Asn Thr Asp Tyr Leu
100 105 110Asp Leu Trp Ser Val Gly Tyr Trp Asp Glu Gly Thr Pro Pro His Glu
115 120 125Val Ala Asp Thr Leu Asp Tyr Ala Val Arg Thr Gly Arg Val Arg Tyr
130 135 140Ala Gly Val Arg Gly Tyr Ser Gly Trp Gln Leu Ala Val Thr His Ala145 150 155 160Ala Ser Asn His Ala Ala Ala Ser Ala Arg Pro Val Val Val Ala Gln
165 170 175Asn Glu Tyr Ser Leu Leu Glu Arg Arg Ala Glu Gln Glu Leu Leu Pro
180 185 190Ala Thr Gln His Leu Gly Val Gly Phe Phe Ala Gly Ala Pro Leu Gly
195 200 205Gln Gly Val Leu Thr Ala Lys Tyr Arg Ser Glu Ile Pro His Asp Ser
210 215 220Arg Ala Ala Ser Thr Gly Arg Asp Ala Glu Val Gln Ser Tyr Leu Asp225 230 235 240Asn Arg Gly Arg Ile Ile Val Asp Ala Leu Asp Thr Ala Ala Lys Gly
245 250 255Leu Gly Ile Ser Pro Ala Val Thr Ala Thr Thr Trp Val Arg Asp Arg
260 265 270Pro Gly Val Thr Ala Val Ile Val Gly Ala Arg Thr His Glu Gln Leu
275 280 285Ser His Leu Leu Lys Ala Glu Ser Val Thr Leu Pro Thr Pro Ile Thr
290 295 300Gln Ala Leu Asp Asp Val Ser Leu305 310<210>753<211>747<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(724)<223>RXN02554<400>753gcttttgaag tgtgtcgcgt gtgcggactg aaatagtttc cgcttcaact tggttgctaa 60ggataggctc cataaaaata accaaaggcg gaaaatttca atg tca cac act aag 115
Met Ser His Thr Lys
1 5cca tcc att gcc atc ctc ggt gct ggc cga gtg ggt tct tca ctt gcc 163Pro Ser Ile Ala Ile Leu Gly Ala Gly Arg Val Gly Ser Ser Leu Ala
10 15 20agg tca gcg gtc gcc gca ggc tat gag gta aag gtt gct ggt tca ggt 211Arg Ser Ala Val Ala Ala Gly Tyr Glu Val Lys Val Ala Gly Ser Gly
25 30 35gct gtg gac aaa atc gct ctt acc gct gag atc ctt atg ccc ggc gcg 259Ala Val Asp Lys Ile Ala Leu Thr Ala Glu Ile Leu Met Pro Gly Ala
40 45 50gtt cca agc act gct gac cag gct gta aag gat gca gat att gtg ttc 307Val Pro Ser Thr Ala Asp Gln Ala Val Lys Asp Ala Asp Ile Val Phe
55 60 65ttg gct gtt ccc ctg cat aaa ttc cgc agt gtc aat cca gcc act tta 355Leu Ala Val Pro Leu His Lys Phe Arg Ser Val Asn Pro Ala Thr Leu70 75 80 85gag ggc aag atc gtt att gac acg atg aac cac tgg gtt ccg gtc aat 403Glu Gly Lys Ile Val Ile Asp Thr Met Asn His Trp Val Pro Val Asn
90 95 100ggt gag ttg gag gaa att gat cag gat ccg cgc agc act tcg gag att 451Gly Glu Leu Glu Glu Ile Asp Gln Asp Pro Arg Ser Thr Ser Glu Ile
105 110 115att gcg gag ttt ttc gcg gga tca acc atg gtg aag tct ttt aac cac 499Ile Ala Glu Phe Phe Ala Gly Ser Thr Met Val Lys Ser Phe Asn His
120 125 130att ggt tat cac gag att gag cag gat gcg ggt acc ggg cgt gcg att 547Ile Gly Tyr His Glu Ile Glu Gln Asp Ala Gly Thr Gly Arg Ala Ile
135 140 145gcg tat gcc acg gat gat gtg gat gca ggt gcc cag gtt gca cag cta 595Ala Tyr Ala Thr Asp Asp Val Asp Ala Gly Ala Gln Val Ala Gln Leu150 155 160 165att aag agt ttt ggg ttt gtt cct tta aat att ggc gca ttg gaa aac 643Ile Lys Ser Phe Gly Phe Val Pro Leu Asn Ile Gly Ala Leu Glu Asn
170 175 180ggc cgt att ctg gaa cct ggc caa gaa gct ttc ggc gcg cac ctt aat 691Gly Arg Ile Leu Glu Pro Gly Gln Glu Ala Phe Gly Ala His Leu Asn
185 190 195aaa gat tcg cgc cta gaa ctt gtt aat cag cgg tagtacctcg atcttcagcc 744Lys Asp Ser Arg Leu Glu Leu Val Asn Gln Arg
200 205aac 747<210>754<211>208<212>PRT<213>谷氨酸棒杆菌<400>754Met Ser His Thr Lys Pro Ser Ile Ala Ile Leu Gly Ala Gly Arg Val1 5 10 15Gly Ser Ser Leu Ala Arg Ser Ala Val Ala Ala Gly Tyr Glu Val Lys
20 25 30Val Ala Gly Ser Gly Ala Val Asp Lys Ile Ala Leu Thr Ala Glu Ile
35 40 45Leu Met Pro Gly Ala Val Pro Ser Thr Ala Asp Gln Ala Val Lys Asp
50 55 60Ala Asp Ile Val Phe Leu Ala Val Pro Leu His Lys Phe Arg Ser Val65 70 75 80Asn Pro Ala Thr Leu Glu Gly Lys Ile Val Ile Asp Thr Met Asn His
85 90 95Trp Val Pro Val Asn Gly Glu Leu Glu Glu Ile Asp Gln Asp Pro Arg
100 105 110Ser Thr Ser Glu Ile Ile Ala Glu Phe Phe Ala Gly Ser Thr Met Val
115 120 125Lys Ser Phe Asn His Ile Gly Tyr His Glu Ile Glu Gln Asp Ala Gly
130 135 140Thr Gly Arg Ala Ile Ala Tyr Ala Thr Asp Asp Val Asp Ala Gly Ala145 150 155 160Gln Val Ala Gln Leu Ile Lys Ser Phe Gly Phe Val Pro Leu Asn Ile
165 170 175Gly Ala Leu Glu Asn Gly Arg Ile Leu Glu Pro Gly Gln Glu Ala Phe
180 185 190Gly Ala His Leu Asn Lys Asp Ser Arg Leu Glu Leu Val Asn Gln Arg
195 200 205<210>755<211>933<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(910)<223>RXN01204<400>755ttacagcgag tttttcagac gtccatcgca ccgtgcacaa caacatttca ggtgcacggc 60ccgaacacgg gagagaacgc tgagcgttac aacactgtcc atg aag ggc gaa ttc 115
Met Lys Gly Glu Phe
1 5cac gcc ccc gat ttg gac aaa gaa ttt ttc ccg ggg cac gta acc gat 163His Ala Pro Asp Leu Asp Lys Glu Phe Phe Pro Gly His Val Thr Asp
10 15 20agt ggt gaa gtc gtg aac atg ctg ttc acc gat ttc gct aat ggt tgg 211Ser Gly Glu Val Val Asn Met Leu Phe Thr Asp Phe Ala Asn Gly Trp
25 30 35ttc gca atg gac cgc atc gta ttg atc cgt ctt ctt atg acg gca gtc 259Phe Ala Met Asp Arg Ile Val Leu Ile Arg Leu Leu Met Thr Ala Val
40 45 50gtt gtg gtc ttc ttc ctt tgg gct atg cgc aag cca aag ctt gtt ccg 307Val Val Val Phe Phe Leu Trp Ala Met Arg Lys Pro Lys Leu Val Pro
55 60 65cat ggc gtc cag aat ttt gca gag tac gca ctc gat ttc gtt ggt att 355His Gly Val Gln Asn Phe Ala Glu Tyr Ala Leu Asp Phe Val Gly Ile70 75 80 85cac atc gct gaa gac atc ctc gga aag aag aaa ggt cgt cgg ttc ctg 403His Ile Ala Glu Asp Ile Leu Gly Lys Lys Lys Gly Arg Arg Phe Leu
90 95 100ccg atc ctg gcc acc atc ttc ttc gcg gct ctg ttg atg aac ctt gca 451Pro Ile Leu Ala Thr Ile Phe Phe Ala Ala Leu Leu Met Asn Leu Ala
105 110 115acg atc atc ccg gga cta aac atc tcc tcc aac tca cgt att gca ttc 499Thr Ile Ile Pro Gly Leu Asn Ile Ser Ser Asn Ser Arg Ile Ala Phe
120 125 130cca atc gtg atg gcg gta gct ggt tac atc gcg ttt atc tac gca ggc 547Pro Ile Val Met Ala Val Ala Gly Tyr Ile Ala Phe Ile Tyr Ala Gly
135 140 145tct aag cgt tac gga ttc ttc aaa tat gtg aag tct tct gtt gtg att 595Ser Lys Arg Tyr Gly Phe Phe Lys Tyr Val Lys Ser Ser Val Val Ile150 155 160 165ccg aac att cca cca gca ctt cac gtc ttg gtg gtt cca att gag ttc 643Pro Asn Ile Pro Pro Ala Leu His Val Leu Val Val Pro Ile Glu Phe
170 175 180ttc tct aca ttc atc ttg agg cca gtc acc ctg gca ctg cgt ttg atg 691Phe Ser Thr Phe Ile Leu Arg Pro Val Thr Leu Ala Leu Arg Leu Met
185 190 195gcc aac ttc ctt gct ggc cac atc atc ctg gtt ctg ctt ttc tcc gca 739Ala Asn Phe Leu Ala Gly His Ile Ile Leu Val Leu Leu Phe Ser Ala
200 205 210acg aac ttc ttc ttc ttc cag ttc aac gga tgg aca gca atg tcc ggc 787Thr Asn Phe Phe Phe Phe Gln Phe Asn Gly Trp Thr Ala Met Ser Gly
215 220 225gta acc atc ttg atg gca gta ctc ttc acg gtt tac gag atc att gtt 835Val Thr Ile Leu Met Ala Val Leu Phe Thr Val Tyr Glu Ile Ile Val230 235 240 245atc ttc ctg cag gca tac atc ttc gct ctg ctg gtc gct gta tac att 883Ile Phe Leu Gln Ala Tyr Ile Phe Ala Leu Leu Val Ala Val Tyr Ile
250 255 260gag ctt tca ctt cac gcg gat tct cac tagatgaaaa aggtcgctat 930Glu Leu Ser Leu His Ala Asp Ser His
265 270taa 933<210>756<211>270<212>PRT<213>谷氨酸棒杆菌<400>756Met Lys Gly Glu Phe His Ala Pro Asp Leu Asp Lys Glu Phe Phe Pro1 5 10 15Gly His Val Thr Asp Ser Gly Glu Val Val Asn Met Leu Phe Thr Asp
20 25 30Phe Ala Asn Gly Trp Phe Ala Met Asp Arg Ile Val Leu Ile Arg Leu
35 40 45Leu Met Thr Ala Val Val Val Val Phe Phe Leu Trp Ala Met Arg Lys
50 55 60Pro Lys Leu Val Pro His Gly Val Gln Asn Phe Ala Glu Tyr Ala Leu65 70 75 80Asp Phe Val Gly Ile His Ile Ala Glu Asp Ile Leu Gly Lys Lys Lys
85 90 95Gly Arg Arg Phe Leu Pro Ile Leu Ala Thr Ile Phe Phe Ala Ala Leu
100 105 110Leu Met Asn Leu Ala Thr Ile Ile Pro Gly Leu Asn Ile Ser Ser Asn
115 120 125Ser Arg Ile Ala Phe Pro Ile Val Met Ala Val Ala Gly Tyr Ile Ala
130 135 140Phe Ile Tyr Ala Gly Ser Lys Arg Tyr Gly Phe Phe Lys Tyr Val Lys145 150 155 160Ser Ser Val Val Ile Pro Asn Ile Pro Pro Ala Leu His Val Leu Val
165 170 175Val Pro Ile Glu Phe Phe Ser Thr Phe Ile Leu Arg Pro Val Thr Leu
180 185 190Ala Leu Arg Leu Met Ala Asn Phe Leu Ala Gly His Ile Ile Leu Val
195 200 205Leu Leu Phe Ser Ala Thr Asn Phe Phe Phe Phe Gln Phe Asn Gly Trp
210 215 220Thr Ala Met Ser Gly Val Thr Ile Leu Met Ala Val Leu Phe Thr Val225 230 235 240Tyr Glu Ile Ile Val Ile Phe Leu Gln Ala Tyr Ile Phe Ala Leu Leu
245 250 255Val Ala Val Tyr Ile Glu Leu Ser Leu His Ala Asp Ser His
260 265 270<210>757<211>862<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(862)<223>FRXA01204<400>757ttacagcgag tttttcagac gtccatcgca ccgtgcacaa caacatttca ggtgcacggc 60ccgaacacgg gagagaacgc tgagcgttac aacactgtcc atg aag ggc gaa ttc 115
Met Lys Gly Glu Phe
1 5cac gcc ccc gat ttg gac aaa gaa ttt ttc ccg ggg cac gta acc gat 163His Ala Pro Asp Leu Asp Lys Glu Phe Phe Pro Gly His Val Thr Asp
10 15 20agt ggt gaa gtc gtg aac atg ctg ttc acc gat ttc gct aat ggt tgg 211Ser Gly Glu Val Val Asn Met Leu Phe Thr Asp Phe Ala Asn Gly Trp
25 30 35ttc gca atg gac cgc atc gta ttg atc cgt ctt ctt atg acg gca gtc 259Phe Ala Met Asp Arg Ile Val Leu Ile Arg Leu Leu Met Thr Ala Val
40 45 50gtt gtg gtc ttc ttc ctt tgg gct atg cgc aag cca aag ctt gtt ccg 307Val Val Val Phe Phe Leu Trp Ala Met Arg Lys Pro Lys Leu Val Pro
55 60 65cat ggc gtc cag aat ttt gca gag tac gca ctc gat ttc gtt ggt att 355His Gly Val Gln Asn Phe Ala Glu Tyr Ala Leu Asp Phe Val Gly Ile70 75 80 85cac atc gct gaa gac atc ctc gga aag aag aaa ggt cgt cgg ttc ctg 403His Ile Ala Glu Asp Ile Leu Gly Lys Lys Lys Gly Arg Arg Phe Leu
90 95 100ccg atc ctg gcc acc atc ttc ttc gcg gct ctg ttg atg aac ctt gca 451Pro Ile Leu Ala Thr Ile Phe Phe Ala Ala Leu Leu Met Asn Leu Ala
105 110 115acg atc atc ccg gga cta aac atc tcc tcc aac tca cgt att gca ttc 499Thr Ile Ile Pro Gly Leu Asn Ile Ser Ser Asn Ser Arg Ile Ala Phe
120 125 130cca atc gtg atg gcg gta gct ggt tac atc gcg ttt atc tac gca ggc 547Pro Ile Val Met Ala Val Ala Gly Tyr Ile Ala Phe Ile Tyr Ala Gly
135 140 145tct aag cgt tac gga ttc ttc aaa tat gtg aag tct tct gtt gtg att 595Ser Lys Arg Tyr Gly Phe Phe Lys Tyr Val Lys Ser Ser Val Val Ile150 155 160 165ccg aac att cca cca gca ctt cac gtc ttg gtg gtt cca att gag ttc 643Pro Asn Ile Pro Pro Ala Leu His Val Leu Val Val Pro Ile Glu Phe
170 175 180ttc tct aca ttc atc ttg agg cca gtc acc ctg gca ctg cgt ttg atg 691Phe Ser Thr Phe Ile Leu Arg Pro Val Thr Leu Ala Leu Arg Leu Met
185 190 195gcc aac ttc ctt gct ggc cac atc atc ctg gtt ctg ctt ttc ttc gca 739Ala Asn Phe Leu Ala Gly His Ile Ile Leu Val Leu Leu Phe Phe Ala
200 205 210acg aac ttc ttc ttc ttc cag ttc aac gga tgg aca gca atg tcc ggc 787Thr Asn Phe Phe Phe Phe Gln Phe Asn Gly Trp Thr Ala Met Ser Gly
215 220 225gta acc atc ttg atg gca gta ctc ttc acg gtt tac gag atc att gtt 835Val Thr Ile Leu Met Ala Val Leu Phe Thr Val Tyr Glu Ile Ile Val230 235 240 245atc ttc ctg cag gca tac atc ttc gct 862Ile Phe Leu Gln Ala Tyr Ile Phe Ala
250<210>758<211>254<212>PRT<213>谷氨酸棒杆菌<400>758Met Lys Gly Glu Phe His Ala Pro Asp Leu Asp Lys Glu Phe Phe Pro1 5 10 15Gly His Val Thr Asp Ser Gly Glu Val Val Asn Met Leu Phe Thr Asp
20 25 30Phe Ala Asn Gly Trp Phe Ala Met Asp Arg Ile Val Leu Ile Arg Leu
35 40 45Leu Met Thr Ala Val Val Val Val Phe Phe Leu Trp Ala Met Arg Lys
50 55 60Pro Lys Leu Val Pro His Gly Val Gln Asn Phe Ala Glu Tyr Ala Leu65 70 75 80Asp Phe Val Gly Ile His Ile Ala Glu Asp Ile Leu Gly Lys Lys Lys
85 90 95Gly Arg Arg Phe Leu Pro Ile Leu Ala Thr Ile Phe Phe Ala Ala Leu
100 105 110Leu Met Asn Leu Ala Thr Ile Ile Pro Gly Leu Asn Ile Ser Ser Asn
115 120 125Ser Arg Ile Ala Phe Pro Ile Val Met Ala Val Ala Gly Tyr Ile Ala
130 135 140Phe Ile Tyr Ala Gly Ser Lys Arg Tyr Gly Phe Phe Lys Tyr Val Lys145 150 155 160Ser Ser Val Val Ile Pro Asn Ile Pro Pro Ala Leu His Val Leu Val
165 170 175Val Pro Ile Glu Phe Phe Ser Thr Phe Ile Leu Arg Pro Val Thr Leu
180 185 190Ala Leu Arg Leu Met Ala Asn Phe Leu Ala Gly His Ile Ile Leu Val
195 200 205Leu Leu Phe Phe Ala Thr Asn Phe Phe Phe Phe Gln Phe Asn Gly Trp
210 215 220Thr Ala Met Ser Gly Val Thr Ile Leu Met Ala Val Leu Phe Thr Val225 230 235 240Tyr Glu Ile Ile Val Ile Phe Leu Gln Ala Tyr Ile Phe Ala
245 250<210>759<211>1764<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1741)<223>RXA01201<400>759tcgggcaaac tcgagcgtct gcgggcaagc ttcgcataaa gacacgacga attagacaac 60attagtaatg ctggaagaaa caaccgagag caggaagaac atg gcg gag ctg acg 115
Met Ala Glu Leu Thr
1 5atc tcc tcc gat gag atc cgt agc gcg att gcg aac tac acc tcg agc 163Ile Ser Ser Asp Glu Ile Arg Ser Ala Ile Ala Asn Tyr Thr Ser Ser
10 15 20tac tcc gcg gag gcc tcc cgt gag gag gtc ggc gtg gtt att tcg gcc 211Tyr Ser Ala Glu Ala Ser Arg Glu Glu Val Gly Val Val Ile Ser Ala
25 30 35gct gac ggt atc gcc cag gtt tcg ggc ctc ccg tca gta atg gcg aat 259Ala Asp Gly Ile Ala Gln Val Ser Gly Leu Pro Ser Val Met Ala Asn
40 45 50gag ctc ctc gaa ttc ccg ggc ggc gtc atc ggc gtc gca cag aac ctt 307Glu Leu Leu Glu Phe Pro Gly Gly Val Ile Gly Val Ala Gln Asn Leu
55 60 65gaa gct gac cga gtc ggc gtc gtg gtc ctg ggt aac tac gag cta ctt 355Glu Ala Asp Arg Val Gly Val Val Val Leu Gly Asn Tyr Glu Leu Leu70 75 80 85aaa gaa ggc gac caa gtt cgt cgt act gga gac gtt ctg tct atc cca 403Lys Glu Gly Asp Gln Val Arg Arg Thr Gly Asp Val Leu Ser Ile Pro
90 95 100gtc ggc gag gca ttc ctt ggc cgc gtt atc aac ccc ctt ggc cag cca 451Val Gly Glu Ala Phe Leu Gly Arg Val Ile Asn Pro Leu Gly Gln Pro
105 110 115att gac ggc ctg ggc gaA att gca tcc gaa gag gac cgc gtc ctc gag 499Ile Asp Gly Leu Gly Glu Ile Ala Ser Glu Glu Asp Arg Val Leu Glu
120 125 130ctt cag gca cca acc gtg ctt gag cgc cag cct gtc gag gag cct ttg 547Leu Gln Ala Pro Thr Val Leu Glu Arg Gln Pro Val Glu Glu Pro Leu
135 140 145gca acc ggc atc aag gct atc gat gca atg acc cca atc ggc cgc ggt 595Ala Thr Gly Ile Lys Ala Ile Asp Ala Met Thr Pro Ile Gly Arg Gly150 155 160 165cag cgt cag ctg atc att ggt gac cgt aag act ggc aag acc gca gtc 643Gln Arg Gln Leu Ile Ile Gly Asp Arg Lys Thr Gly Lys Thr Ala Val
170 175 180tgt gtc gat acc atc ctt aac cag aag gcc aac tgg gag acc ggc gac 691Cys Val Asp Thr Ile Leu Asn Gln Lys Ala Asn Trp Glu Thr Gly Asp
185 190 195aag acc aag cag gtt cgc tgc atc tac gtc gca atc ggt cag aag ggc 739Lys Thr Lys Gln Val Arg Cys Ile Tyr Val Ala Ile Gly Gln Lys Gly
200 205 210tcc acc att gca gcc ctg cgt aag acc ctc gag gag cag ggc gct ctc 787Ser Thr Ile Ala Ala Leu Arg Lys Thr Leu Glu Glu Gln Gly Ala Leu
215 220 225gag tac acc acc atc gtg gct gca ccc gct tcc gat gct gca ggc ttc 835Glu Tyr Thr Thr Ile Val Ala Ala Pro Ala Ser Asp Ala Ala Gly Phe230 235 240 245aag tgg ctt gca cca ttc gct ggc gct gct ctc gcc cag cac tgg atg 883Lys Trp Leu Ala Pro Phe Ala Gly Ala Ala Leu Ala Gln His Trp Met
250 255 260tac cag ggc aac cac gtc ctg gtc atc tac gat gat ctg acc aag cag 931Tyr Gln Gly Asn His Val Leu Val Ile Tyr Asp Asp Leu Thr Lys Gln
265 270 275gct gag gca tac cgt gct atc tcc ctg ctg ctg cgt cgc cca ccg ggc 979Ala Glu Ala Tyr Arg Ala Ile Ser Leu Leu Leu Arg Arg Pro Pro Gly
280 285 290cgc gaa gca tac cca ggt gac gte ttc tac ctg cac tcc cgt ctg ctg 1027Arg Glu Ala Tyr Pro Gly Asp Val Phe Tyr Leu His Ser Arg Leu Leu
295 300 305gag cgc gct gcg aag ctg tcc gat gaa cta ggc gca ggt tct att aca 1075Glu Arg Ala Ala Lys Leu Ser Asp Glu Leu Gly Ala Gly Ser Ile Thr310 315 320 325gca ctg cca atc att gag acc aag gct aat gac gtt tcc gcc ttc att 1123Ala Leu Pro Ile Ile Glu Thr Lys Ala Asn Asp Val Ser Ala Phe Ile
330 335 340cct acc aac gtg att tcc atc acc gac ggt cag gta ttc ctt gag tcc 1171Pro Thr Asn Val Ile Ser Ile Thr Asp Gly Gln Val Phe Leu Glu Ser
345 350 355gac ctg ttc aac cgt ggc gtt cgc ccg gcg atc aac gtc ggt gta tcc 1219Asp Leu Phe Asn Arg Gly Val Arg Pro Ala Ile Asn Val Gly Val Ser
360 365 370gtc tcc cgt gtc ggt ggc gca gct cag acc aag ggt atg aag aag gtt 1267Val Ser Arg Val Gly Gly Ala Ala Gln Thr Lys Gly Met Lys Lys Val
375 380 385gcc ggt tct ctc cgt ctg gat ctg gct gca ttc cgc gac ctg gaa gca 1315Ala Gly Ser Leu Arg Leu Asp Leu Ala Ala Phe Arg Asp Leu Glu Ala390 395 400 405ttc gct acc ttc gca tct gac ttg gat gct gca tcc aag tct cag ctt 1363Phe Ala Thr Phe Ala Ser Asp Leu Asp Ala Ala Ser Lys Ser Gln Leu
410 415 420gag cgt ggc cag cgc ctc gtt cag ctg ttg att cag tct gag aac gca 1411Glu Arg Gly Gln Arg Leu Val Gln Leu Leu Ile Gln Ser Glu Asn Ala
425 430 435cct cag gct gtt gag tac cag atc att tct ctc tgg ctt gca ggc gaa 1459Pro Gln Ala Val Glu Tyr Gln Ile Ile Ser Leu Trp Leu Ala Gly Glu
440 445 450ggc gca ttc gac aac gtt cct gtt gaa gat gtt cgt cgc ttc gag tcc 1507Gly Ala Phe Asp Asn Val Pro Val Glu Asp Val Arg Arg Phe Glu Ser
455 460 465gaa ctg cac gag tac tta ggc tcc aac gct gca cag gtc tac gag cag 1555Glu Leu His Glu Tyr Leu Gly Ser Asn Ala Ala Gln Val Tyr Glu Gln470 475 480 485atc gct ggt gga gct cag ctt tcc gac gag tcc aag gaa acc ttg ctc 1603Ile Ala Gly Gly Ala Gln Leu Ser Asp Glu Ser Lys Glu Thr Leu Leu
490 495 500aag gca acc gaa gat ttc aag agc gct ttc cag acc acc gat ggc acc 1651Lys Ala Thr Glu Asp Phe Lys Ser Ala Phe Gln Thr Thr Asp Gly Thr
505 510 515cct gtc atc aac gag cct gag gtt gaa gca ctc gat gca ggc cag gtc 1699Pro Val Ile Asn Glu Pro Glu Val Glu Ala Leu Asp Ala Gly Gln Val
520 525 530aag aaa gac cag ctc acc gtt tcc cgc aag gtc agc aag aag 1741Lys Lys Asp Gln Leu Thr Val Ser Arg Lys Val Ser Lys Lys
535 540 545taaggcagcg agcctacact aaa 1764<210>760<211>547<212>PRT<213>谷氨酸棒杆菌<400>760Met Ala Glu Leu Thr Ile Ser Ser Asp Glu Ile Arg Ser Ala Ile Ala1 5 10 15Asn Tyr Thr Ser Ser Tyr Ser Ala Glu Ala Ser Arg Glu Glu Val Gly
20 25 30Val Val Ile Ser Ala Ala Asp Gly Ile Ala Gln Val Ser Gly Leu Pro
35 40 45Ser Val Met Ala Asn Glu Leu Leu Glu Phe Pro Gly Gly Val Ile Gly
50 55 60Val Ala Gln Asn Leu Glu Ala Asp Arg Val Gly Val Val Val Leu Gly65 70 75 80Asn Tyr Glu Leu Leu Lys Glu Gly Asp Gln Val Arg Arg Thr Gly Asp
85 90 95Val Leu Ser Ile Pro Val Gly Glu Ala Phe Leu Gly Arg Val Ile Asn
100 105 110Pro Leu Gly Gln Pro Ile Asp Gly Leu Gly Glu Ile Ala Ser Glu Glu
115 120 125Asp Arg Val Leu Glu Leu Gln Ala Pro Thr Val Leu Glu Arg Gln Pro
130 135 140Val Glu Glu Pro Leu Ala Thr Gly Ile Lys Ala Ile Asp Ala Met Thr145 150 155 160Pro Ile Gly Arg Gly Gln Arg Gln Leu Ile Ile Gly Asp Arg Lys Thr
165 170 175Gly Lys Thr Ala Val Cys Val Asp Thr Ile Leu Asn Gln Lys Ala Asn
180 185 190Trp Glu Thr Gly Asp Lys Thr Lys Gln Val Arg Cys Ile Tyr Val Ala
195 200 205Ile Gly Gln Lys Gly Ser Thr Ile Ala Ala Leu Arg Lys Thr Leu Glu
210 215 220Glu Gln Gly Ala Leu Glu Tyr Thr Thr Ile Val Ala Ala Pro Ala Ser225 230 235 240Asp Ala Ala Gly Phe Lys Trp Leu Ala Pro Phe Ala Gly Ala Ala Leu
245 250 255Ala Gln His Trp Met Tyr Gln Gly Asn His Val Leu Val Ile Tyr Asp
260 265 270Asp Leu Thr Lys Gln Ala Glu Ala Tyr Arg Ala Ile Ser Leu Leu Leu
275 280 285Arg Arg Pro Pro Gly Arg Glu Ala Tyr Pro Gly Asp Val Phe Tyr Leu
290 295 300His Ser Arg Leu Leu Glu Arg Ala Ala Lys Leu Ser Asp Glu Leu Gly305 310 315 320Ala Gly Ser Ile Thr Ala Leu Pro Ile Ile Glu Thr Lys Ala Asn Asp
325 330 335Val Ser Ala Phe Ile Pro Thr Asn Val Ile Ser Ile Thr Asp Gly Gln
340 345 350Val Phe Leu Glu Ser Asp Leu Phe Asn Arg Gly Val Arg Pro Ala Ile
355 360 365Asn Val Gly Val Ser Val Ser Arg Val Gly Gly Ala Ala Gln Thr Lys
370 375 380Gly Met Lys Lys Val Ala Gly Ser Leu Arg Leu Asp Leu Ala Ala Phe385 390 395 400Arg Asp Leu Glu Ala Phe Ala Thr Phe Ala Ser Asp Leu Asp Ala Ala
405 410 415Ser Lys Ser Gln Leu Glu Arg Gly Gln Arg Leu Val Gln Leu Leu Ile
420 425 430Gln Ser Glu Asn Ala Pro Gln Ala Val Glu Tyr Gln Ile Ile Ser Leu
435 440 445Trp Leu Ala Gly Glu Gly Ala Phe Asp Asn Val Pro Val Glu Asp Val
450 455 460Arg Arg Phe Glu Ser Glu Leu His Glu Tyr Leu Gly Ser Asn Ala Ala465 470 475 480Gln ValTyr Glu Gln Ile Ala Gly Gly Ala Gln Leu Ser Asp Glu Ser
485 490 495Lys Glu Thr Leu Leu Lys Ala Thr Glu Asp Phe Lys Ser Ala Phe Gln
500 505 510Thr Thr Asp Gly Thr Pro Val Ile Asn Glu Pro Glu Val Glu Ala Leu
515 520 525Asp Ala Gly Gln Val Lys Lys Asp Gln Leu Thr Val Ser Arg Lys Val
530 535 540Ser Lys Lys545<210>761<211>1572<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1549)<223>RXN01193<400>761gtgtggccaa ccaggcacgt caggcacaga tcacccagga aatcacagag attgttggtg 60gcgcaggcgc gctcgccgac agcggagaaa gtgactaatt atg act aca gct ctt 115
Met Thr Thr Ala Leu
1 5gaa gag cag aac gca cag cag gca gcc act gcc ggc cgt gtc gtg cgt 163Glu Glu Gln Asn Ala Gln Gln Ala Ala Thr Ala Gly Arg Val Val Arg
10 15 20gtc att ggt gcg gtc gtc gac gtg gag ttt ccc cgc ggc gag ctg cca 211Val Ile Gly Ala Val Val Asp Val Glu Phe Pro Arg Gly Glu Leu Pro
25 30 35gca ctg tac aac gca ctt act gta gag gta acc ctc gaa tca gtt aag 259Ala Leu Tyr Asn Ala Leu Thr Val Glu Val Thr Leu Glu Ser Val Lys
40 45 50aag acc gtt gtt ctc gag gtt gct cag cac ctc ggc gac aac ctc atc 307Lys Thr Val Val Leu Glu Val Ala Gln His Leu Gly Asp Asn Leu Ile
55 60 65cgc acc atc gct atg gca cca acc gac gga ctt gtc cgc ggt gct gct 355Arg Thr Ile Ala Met Ala Pro Thr Asp Gly Leu Val Arg Gly Ala Ala70 75 80 85gta acc gat act gca cgc cca att tcc gta cca gtg ggc gat gtt gtt 403Val Thr Asp Thr Ala Arg Pro Ile Ser Val Pro Val Gly Asp Val Val
90 95 100aag ggc cac gta ttc aac gct ttg ggc gac tgc cta gac gac gtt tcc 451Lys Gly His Val Phe Asn Ala Leu Gly Asp Cys Leu Asp Asp Val Ser
105 110 115ctg aac aac aac cca gag atc gag cgt tgg ggc atc cac cgc gag cca 499Leu Asn Asn Asn Pro Glu Ile Glu Arg Trp Gly Ile His Arg Glu Pro
120 125 130cca tca ttc gat cag ctt gag ggt aag acc gag atc ctg gaa aca ggc 547Pro Ser Phe Asp Gln Leu Glu Gly Lys Thr Glu Ile Leu Glu Thr Gly
135 140 145atc aag gtt atc gac ctt ctc acc cct tac gtt aag ggt gga aag atc 595Ile Lys Val Ile Asp Leu Leu Thr Pro Tyr Val Lys Gly Gly Lys Ile150 155 160 165ggc ctc ttc ggt ggt gca ggt gtg ggt aag acc gtt ctt atc cag gaa 643Gly Leu Phe Gly Gly Ala Gly Val Gly Lys Thr Val Leu Ile Gln Glu
170 175 180atg atc acc cgt att gca cgt gag ttc tcc ggt act tcc gtg ttc gca 691Met Ile Thr Arg Ile Ala Arg Glu Phe Ser Gly Thr Ser Val Phe Ala
185 190 195ggt gtt ggt gag cgt acc cgt gag ggc acc gac ctc ttc ctc gaa atg 739Gly Val Gly Glu Arg Thr Arg Glu Gly Thr Asp Leu Phe Leu Glu Met
200 205 210gaa gaa atg ggc gtt ctc cag gac acc gcc ctg gtg ttc ggt cag atg 787Glu Glu Met Gly Val Leu Gln Asp Thr Ala Leu Val Phe Gly Gln Met
215 220 225gat gag cca cca gga gtc ggt atg cgc gtg gct ctg tcc ggc ctg acc 835Asp Glu Pro Pro Gly Val Gly Met Arg Val Ala Leu Ser Gly Leu Thr230 235 240 245atg gcg gag tac ttc cgc gat gtt cag aac cag gac gtg ctg ctg ttc 883Met Ala Glu Tyr Phe Arg Asp Val Gln Asn Gln Asp Val Leu Leu Phe
250 255 260atc gac aac atc ttc cgt ttc acc cag gca ggt tct gag gtt tcc acc 931Ile Asp Asn Ile Phe Arg Phe Thr Gln Ala Gly Ser Glu Val Ser Thr
265 270 275ctt ctg ggt cgt atg cct tcc gcc gtg ggt tac cag cca acc ctg gct 979Leu Leu Gly Arg Met Pro Ser Ala Val Gly Tyr Gln Pro Thr Leu Ala
280 285 290gac gag atg ggt gtt ctc cag gag cgc att acc tcc acc aag ggc cgt 1027Asp Glu Met Gly Val Leu Gln Glu Arg Ile Thr Ser Thr Lys Gly Arg
295 300 305tcg att acc tct ctg cag gcc gtt tac gtt cct gcc gat gac tac acc 1075Ser Ile Thr Ser Leu Gln Ala Val Tyr Val Pro Ala Asp Asp Tyr Thr310 315 320 325gac ccg gct cca gcg acc acc ttc gct cac ttg gat gca acc acc gag 1123Asp Pro Ala Pro Ala Thr Thr Phe Ala His Leu Asp Ala Thr Thr Glu
330 335 340ctt gac cgc tcc att gct tcc aag ggt att tac cca gca gtg aac cca 1171Leu Asp Arg Ser Ile Ala Ser Lys Gly Ile Tyr Pro Ala Val Asn Pro
345 350 355ctg acc tcc acc tct cgt att ctc gag cca gca atc gtt ggt gag cgt 1219Leu Thr Ser Thr Ser Arg Ile Leu Glu Pro Ala Ile Val Gly Glu Arg
360 365 370cac tac gag gtt tct cag cgt gtc atc ggc att ctg cag aag aac aag 1267His Tyr Glu Val Ser Gln Arg Val Ile Gly Ile Leu Gln Lys Asn Lys
375 380 385gaa ctt cag gac atc atc gcc atc ctt ggt atg gac gag ctt tct gaa 1315Glu Leu Gln Asp Ile Ile Ala Ile Leu Gly Met Asp Glu Leu Ser Glu390 395 400 405gag gac aag atc acc gtt gca cgt gcg cgt cgc atc gag cgc ttc ctg 1363Glu Asp Lys Ile Thr Val Ala Arg Ala Arg Arg Ile Glu Arg Phe Leu
410 415 420ggt cag aac ttc ttc gtt gca gag aag ttc acc ggt ctt cct ggc tcc 1411Gly Gln Asn Phe Phe Val Ala Glu Lys Phe Thr Gly Leu Pro Gly Ser
425 430 435tac gtg cca ctg acc gac acc gtc gac gct ttc gag cgt att tgc aac 1459Tyr Val Pro Leu Thr Asp Thr Val Asp Ala Phe Glu Arg Ile Cys Asn
440 445 450ggc gac ttc gac cac tac cca gag cag gct ttc aac ggc ctc ggt ggt 1507Gly Asp Phe Asp His Tyr Pro Glu Gln Ala Phe Asn Gly Leu Gly Gly
455 460 465ttg gac gat gtc gaa gct gca tac aag aag ctg acc gga aag 1549Leu Asp Asp Val Glu Ala Ala Tyr Lys Lys Leu Thr Gly Lys470 475 480taaggtagag acacatggct gaa 1572<210>762<211>483<212>PRT<213>谷氨酸棒杆菌<400>762Met Thr Thr Ala Leu Glu Glu Gln Asn Ala Gln Gln Ala Ala Thr Ala1 5 10 15Gly Arg Val Val Arg Val Ile Gly Ala Val Val Asp Val Glu Phe Pro
20 25 30Arg Gly Glu Leu Pro Ala Leu Tyr Asn Ala Leu Thr Val Glu Val Thr
35 40 45Leu Glu Ser Val Lys Lys Thr Val Val Leu Glu Val Ala Gln His Leu
50 55 60Gly Asp Asn Leu Ile Arg Thr Ile Ala Met Ala Pro Thr Asp Gly Leu65 70 75 80Val Arg Gly Ala Ala Val Thr Asp Thr Ala Arg Pro Ile Ser Val Pro
85 90 95Val Gly Asp Val Val Lys Gly His Val Phe Asn Ala Leu Gly Asp Cys
100 105 110Leu Asp Asp Val Ser Leu Asn Asn Asn Pro Glu Ile Glu Arg Trp Gly
115 120 125Ile His Arg Glu Pro Pro Ser Phe Asp Gln Leu Glu Gly Lys Thr Glu
130 135 140Ile Leu Glu Thr Gly Ile Lys Val Ile Asp Leu Leu Thr Pro Tyr Val145 150 155 160Lys Gly Gly Lys Ile Gly Leu Phe Gly Gly Ala Gly Val Gly Lys Thr
165 170 175Val Leu Ile Gln Glu Met Ile Thr Arg Ile Ala Arg Glu Phe Ser Gly
180 185 190Thr Ser Val Phe Ala Gly Val Gly Glu Arg Thr Arg Glu Gly Thr Asp
195 200 205Leu Phe Leu Glu Met Glu Glu Met Gly Val Leu Gln Asp Thr Ala Leu
210 215 220Val Phe Gly Gln Met Asp Glu Pro Pro Gly Val Gly Met Arg Val Ala225 230 235 240Leu Ser Gly Leu Thr Met Ala Glu Tyr Phe Arg Asp Val Gln Asn Gln
245 250 255Asp Val Leu Leu Phe Ile Asp Asn Ile Phe Arg Phe Thr Gln Ala Gly
260 265 270Ser Glu Val Ser Thr Leu Leu Gly Arg Met Pro Ser Ala Val Gly Tyr
275 280 285Gln Pro Thr Leu Ala Asp Glu Met Gly Val Leu Gln Glu Arg Ile Thr
290 295 300Ser Thr Lys Gly Arg Ser Ile Thr Ser Leu Gln Ala Val Tyr Val Pro305 310 315 320Ala Asp Asp Tyr Thr Asp Pro Ala Pro Ala Thr Thr Phe Ala His Leu
325 330 335Asp Ala Thr Thr Glu Leu Asp Arg Ser Ile Ala Ser Lys Gly Ile Tyr
340 345 350Pro Ala Val Asn Pro Leu Thr Ser Thr Ser Arg Ile Leu Glu Pro Ala
355 360 365Ile Val Gly Glu Arg His Tyr Glu Val Ser Gln Arg Val Ile Gly Ile
370 375 380Leu Gln Lys Asn Lys Glu Leu Gln Asp Ile Ile Ala Ile Leu Gly Met385 390 395 400Asp Glu Leu Ser Glu Glu Asp Lys Ile Thr Val Ala Arg Ala Arg Arg
405 410 415Ile Glu Arg Phe Leu Gly Gln Asn Phe Phe Val Ala Glu Lys Phe Thr
420 425 430Gly Leu Pro Gly Ser Tyr Val Pro Leu Thr Asp Thr Val Asp Ala Phe
435 440 445Glu Arg Ile Cys Asn Gly Asp Phe Asp His Tyr Pro Glu Gln Ala Phe
450 455 460Asn Gly Leu Gly Gly Leu Asp Asp Val Glu Ala Ala Tyr Lys Lys Leu465 470 475 480Thr Gly Lys<210>763<211>778<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(15)..(755)<223>FRXA01193<400>763caccaggagt cggt atg cgc gtg gct ctg tcc ggc ctg acc atg gcg gag 50
Met Arg Val Ala Leu Ser Gly Leu Thr Met Ala Glu
1 5 10tac ttc cgc gat gtt cag aac cag gac gtg ctg ctg ttc atc gac aac 98Tyr Phe Arg Asp Val Gln Asn Gln Asp Val Leu Leu Phe Ile Asp Asn
15 20 25atc ttc cgt ttc acc cag gca ggt tct gag gtt tcc acc ctt ctg ggt 146Ile Phe Arg Phe Thr Gln Ala Gly Ser Glu Val Ser Thr Leu Leu Gly
30 35 40cgt atg cct tcc gcc gtg ggt tac cag cca acc ctg gct gac gag atg 194Arg Met Pro Ser Ala Val Gly Tyr Gln Pro Thr Leu Ala Asp Glu Met45 50 55 60ggt gtt ctc cag gag cgc att acc tcc acc aag ggc cgt tcg att acc 242Gly Val Leu Gln Glu Arg Ile Thr Ser Thr Lys Gly Arg Ser Ile Thr
65 70 75tct ctg cag gcc gtt tac gtt cct gcc gat gac tac acc gac ccg gct 290Ser Leu Gln Ala Val Tyr Val Pro Ala Asp Asp Tyr Thr Asp Pro Ala
80 85 90cca gcg acc acc ttc gct cac ttg gat gca acc acc gag ctt gac cgc 338Pro Ala Thr Thr Phe Ala His Leu Asp Ala Thr Thr Glu Leu Asp Arg
95 100 105tcc att gct tcc aag ggt att tac cca gca gtg aac cca ctg acc tcc 386Ser Ile Ala Ser Lys Gly Ile Tyr Pro Ala Val Asn Pro Leu Thr Ser
110 115 120acc tct cgt att ctc gag cca gca atc gtt ggt gag cgt cac tac gag 434Thr Ser Arg Ile Leu Glu Pro Ala Ile Val Gly Glu Arg His Tyr Glu125 130 135 140gtt tct cag cgt gtc atc ggc att ctg cag aag aac aag gaa ctt cag 482Val Ser Gln Arg Val Ile Gly Ile Leu Gln Lys Asn Lys Glu Leu Gln
145 150 155gac atc atc gcc atc ctt ggt atg gac gag ctt tct gaa gag gac aag 530Asp Ile Ile Ala Ile Leu Gly Met Asp Glu Leu Ser Glu Glu Asp Lys
160 165 170atc acc gtt gca cgt gcg cgt cgc atc gag cgc ttc ctg ggt cag aac 578Ile Thr Val Ala Arg Ala Arg Arg Ile Glu Arg Phe Leu Gly Gln Asn
175 180 185ttc ttc gtt gca gag aag ttc acc ggt ctt cct ggc tcc tac gtg cca 626Phe Phe Val Ala Glu Lys Phe Thr Gly Leu Pro Gly Ser Tyr Val Pro
190 195 200ctg acc gac acc gtc gac gct ttc gag cgt att tgc aac ggc gac ttc 674Leu Thr Asp Thr Val Asp Ala Phe Glu Arg Ile Cys Asn Gly Asp Phe205 210 215 220gac cac tac cca gag cag gct ttc aac ggc ctc ggt ggt ttg gac gat 722Asp His Tyr Pro Glu Gln Ala Phe Asn Gly Leu Gly Gly Leu Asp Asp
225 230 235gtc gaa gct gca tac aag aag ctg acc gga aag taaggtagag acacatggct 775Val Glu Ala Ala Tyr Lys Lys Leu Thr Gly Lys
240 245gaa 778<210>764<211>247<212>PRT<213>谷氨酸棒杆菌<400>764Met Arg Val Ala Leu Ser Gly Leu Thr Met Ala Glu Tyr Phe Arg Asp1 5 10 15Val Gln Asn Gln Asp Val Leu Leu Phe Ile Asp Asn Ile Phe Arg Phe
20 25 30Thr Gln Ala Gly Ser Glu Va1 Ser Thr Leu Leu Gly Arg Met Pro Ser
35 40 45Ala Val Gly Tyr Gln Pro Thr Leu Ala Asp Glu Met Gly Val Leu Gln
50 55 60Glu Arg Ile Thr Ser Thr Lys Gly Arg Ser Ile Thr Ser Leu Gln Ala65 70 75 80Val Tyr Val Pro Ala Asp Asp Tyr Thr Asp Pro Ala Pro Ala Thr Thr
85 90 95Phe Ala His Leu Asp Ala Thr Thr Glu Leu Asp Arg Ser Ile Ala Ser
100 105 110Lys Gly Ile Tyr Pro Ala Val Asn Pro Leu Thr Ser Thr Ser Arg Ile
115 120 125Leu Glu Pro Ala Ile Val Gly Glu Arg His Tyr Glu Val Ser Gln Arg
130 135 140Val Ile Gly Ile Leu Gln Lys Asn Lys Glu Leu Gln Asp Ile Ile Ala145 150 155 160Ile Leu Gly Met Asp Glu Leu Ser Glu Glu Asp Lys Ile Thr Val Ala
165 170 175Arg Ala Arg Arg Ile Glu Arg Phe Leu Gly Gln Asn Phe Phe Val Ala
180 185 190Glu Lys Phe Thr Gly Leu Pro Gly Ser Tyr Val Pro Leu Thr Asp Thr
195 200 205Val Asp Ala Phe Glu Arg Ile Cys Asn Gly Asp Phe Asp His Tyr Pro
210 215 220Glu Gln Ala Phe Asn Gly Leu Gly Gly Leu Asp Asp Val Glu Ala Ala225 230 235 240Tyr Lys Lys Leu Thr Gly Lys
245<210>765<211>739<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(739)<223>FRXA01203<400>765gtgtggccaa ccaggcacgt caggcacaga tcacccagga aatcacagag attgttggtg 60gcgcaggcgc gctcgccgac agcggagaaa gtgactaatt atg act aca gct ctt 115
Met Thr Thr Ala Leu
1 5gaa gag cag aac gca cag cag gca gcc act gcc ggc cgt gtc gtg cgt 163Glu Glu Gln Asn Ala Gln Gln Ala Ala Thr Ala Gly Arg Val Val Arg
10 15 20gtc att ggt gcg gtc gtc gac gtg gag ttt ccc cgc ggc gag ctg cca 211Val Ile Gly Ala Val Val Asp Val Glu Phe Pro Arg Gly Glu Leu Pro
25 30 35gca ctg tac aac gca ctt act gta gag gta acc ctc gaa tca gtt aag 259Ala Leu Tyr Asn Ala Leu Thr Val Glu Val Thr Leu Glu Ser Val Lys
40 45 50aag acc gtt gtt ctc gag gtt gct cag cac ctc ggc gac aac ctc atc 307Lys Thr Val Val Leu Glu Val Ala Gln His Leu Gly Asp Asn Leu Ile
55 60 65cgc acc atc gct atg gca cca acc gac gga ctt gtc cgc ggt gct gct 355Arg Thr Ile Ala Met Ala Pro Thr Asp Gly Leu Val Arg Gly Ala Ala70 75 80 85gta acc gat act gca cgc cca att tcc gta cca gtg ggc gat gtt gtt 403Val Thr Asp Thr Ala Arg Pro Ile Ser Val Pro Val Gly Asp Val Val
90 95 100aag ggc cac gta ttc aac gct ttg ggc gac tgc cta gac gac gtt tcc 451Lys Gly His Val Phe Asn Ala Leu Gly Asp Cys Leu Asp Asp Val Ser
105 110 115ctg aac aac aac cca gag atc gag cgt tgg ggc atc cac cgc gag cca 499Leu Asn Asn Asn Pro Glu Ile Glu Arg Trp Gly Ile His Arg Glu Pro
120 125 130cca tca ttc gat cag ctt gag ggt aag acc gag atc ctg gaa aca ggc 547Pro Ser Phe Asp Gln Leu Glu Gly Lys Thr Glu Ile Leu Glu Thr Gly
135 140 145atc aag gtt atc gac ctt ctc acc cct tac gtt aag ggt gga aag atc 595Ile Lys Val Ile Asp Leu Leu Thr Pro Tyr Val Lys Gly Gly Lys Ile150 155 160 165ggc ctc ttc ggt ggt gca ggt gtg ggt aag acc gtt ctt atc cag gaa 643Gly Leu Phe Gly Gly Ala Gly Val Gly Lys Thr Val Leu Ile Gln Glu
170 175 180atg ate acc cgt att gca cgt gag ttc tcc ggt act tcc gtg ttc gca 691Met Ile Thr Arg Ile Ala Arg Glu Phe Ser Gly Thr Ser Val Phe Ala
185 190 195ggt gtt ggt aag cgt acc cgt gag ggc acc gac ctc ttc ctc gaa atg 739Gly Val Gly Lys Arg Thr Arg Glu Gly Thr Asp Leu Phe Leu Glu Met
200 205 210<210>766<211>213<212>PRT<213>谷氨酸棒杆菌<400>766Met Thr Thr Ala Leu Glu Glu Gln Asn Ala Gln Gln Ala Ala Thr Ala1 5 10 15Gly Arg Val Val Arg Val Ile Gly Ala Val Val Asp Val Glu Phe Pro
20 25 30Arg Gly Glu Leu Pro Ala Leu Tyr Asn Ala Leu Thr Val Glu Val Thr
35 40 45Leu Glu Ser Val Lys Lys Thr Val Val Leu Glu Val Ala Gln His Leu
50 55 60Gly Asp Asn Leu Ile Arg Thr Ile Ala Met Ala Pro Thr Asp Gly Leu65 70 75 80Val Arg Gly Ala Ala Val Thr Asp Thr Ala Arg Pro Ile Ser Val Pro
85 90 95Val Gly Asp Val Val Lys Gly His Val Phe Asn Ala Leu Gly Asp Cys
100 105 110Leu Asp Asp Val Ser Leu Asn Asn Asn Pro Glu Ile Glu Arg Trp Gly
115 120 125Ile His Arg Glu Pro Pro Ser Phe Asp Gln Leu Glu Gly Lys Thr Glu
130 135 140Ile Leu Glu Thr Gly Ile Lys Val Ile Asp Leu Leu Thr Pro Tyr Val145 150 155 160Lys Gly Gly Lys Ile Gly Leu Phe Gly Gly Ala Gly Val Gly Lys Thr
165 170 175Val Leu Ile Gln Glu Met Ile Thr Arg Ile Ala Arg Glu Phe Ser Gly
180 185 190Thr Ser Val Phe Ala Gly Val Gly Lys Arg Thr Arg Glu Gly Thr Asp
195 200 205Leu Phe Leu Glu Met
210<210>767<211>363<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(340)<223>RXN02821<400>767aagtcgccca gatagcgagc ggaccactcg gtcaactgaa taaccccact aaacacttca 60cagcccgaac acacgggcac cagaaaggga acgacacctc atg aac gag atc atc 115
Met Asn Glu Ile Ile
1 5ctg gca cag gac gca acc gag tcc acc atc acc gga ctt ggc gct gtc 163Leu Ala Gln Asp Ala Thr Glu Ser Thr Ile Thr Gly Leu Gly Ala Val
10 15 20ggc tac ggc atc gca acc atc gga cct ggc ctc ggc atc ggc atc ctg 211Gly Tyr Gly Ile Ala Thr Ile Gly Pro Gly Leu Gly Ile Gly Ile Leu
25 30 35gtt ggt aag gct ctc gag ggt atg gca cgt cag cct gag atg gct gga 259Val Gly Lys Ala Leu Glu Gly Met Ala Arg Gln Pro Glu Met Ala Gly
40 45 50cag ctc cgt acc acc atg ttc ctg ggc atc gcc ttc gtt gag gcc ctg 307Gln Leu Arg Thr Thr Met Phe Leu Gly Ile Ala Phe Val Glu Ala Leu
55 60 65gca ctg atc ggc ctt gtt gct ggc ttc ctg ttc taatcagcta acttaaccga 360Ala Leu Ile Gly Leu Val Ala Gly Phe Leu Phe70 75 80aag 363<210>768<211>80<212>PRT<213>谷氨酸棒杆菌<400>768Met Asn Glu Ile Ile Leu Ala Gln Asp Ala Thr Glu Ser Thr Ile Thr1 5 10 15Gly Leu Gly Ala Val Gly Tyr Gly Ile Ala Thr Ile Gly Pro Gly Leu
20 25 30Gly Ile Gly Ile Leu Val Gly Lys Ala Leu Glu Gly Met Ala Arg Gln
35 40 45Pro Glu Met Ala Gly Gln Leu Arg Thr Thr Met Phe Leu Gly Ile Ala
50 55 60Phe Val Glu Ala Leu Ala Leu Ile Gly Leu Val Ala Gly Phe Leu Phe65 70 75 80<210>769<211>303<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(280)<223>FRXA02821<400>769agcccgaaca cacgggcacc agaaagggaa cgacacctca tgaacgagat catcttggca 60caggacgcaa ccgagtccac catcaccgga ccttggcgct gtg ggo tac ggc atc 115
Val Gly Tyr Gly Ile
1 5gca acc atc gga cct ggc ctc ggc atc ggc atc ttg gtt ggt aag gct 163Ala Thr Ile Gly Pro Gly Leu Gly Ile Gly Ile Leu Val Gly Lys Ala
10 15 20ctc gag ggt atg gca cgt cag cct gag atg gct gga cag ctc cgt acc 211Leu Glu Gly Met Ala Arg Gln Pro Glu Met Ala Gly Gln Leu Arg Thr
25 30 35acc atg ttc ctg ggc atc gcc ttc gtt gag gcc ctg gca ctg atc ggc 259Thr Met Phe Leu Gly Ile Ala Phe Val Glu Ala Leu Ala Leu Ile Gly
40 45 50ctt gtt gct ggc ttc ctg ttc taatcagcta acttaaccga aag 303Leu Val Ala Gly Phe Leu Phe
55 60<210>770<211>60<212>PRT<213>谷氨酸棒杆菌<400>770Val Gly Tyr Gly Ile Ala Thr Ile Gly Pro Gly Leu Gly Ile Gly Ile1 5 10 15Leu Val Gly Lys Ala Leu Glu Gly Met Ala Arg Gln Pro Glu Met Ala
20 25 30Gly Gln Leu Arg Thr Thr Met Phe Leu Gly Ile Ala Phe Val Glu Ala
35 40 45Leu Ala Leu Ile Gly Leu Val Ala Gly Phe Leu Phe
50 55 60<210>771<211>632<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(1)..(609)<223>RXA01200<400>771ggc tgt ctt cgg tgg aaa agt gag cca tct gtt ctc gaa gtc ctc aag 48Gly Cys Leu Arg Trp Lys Ser Glu Pro Ser Val Leu Glu Val Leu Lys1 5 10 15gac gcc gca gag cag acc tgg tcc act cca cgc gag ttc cgc gct gga 96Asp Ala Ala Glu Gln Thr Trp Ser Thr Pro Arg Glu Phe Arg Ala Gly
20 25 30cta gtc caa ctt ggc cgt cgc gcc ctt ctt cgc tct gcg gag aaa cag 144Leu Val Gln Leu Gly Arg Arg Ala Leu Leu Arg Ser Ala Glu Lys Gln
35 40 45ggt cag ctt ggt cag gtg gaa gat gaa ctg ttc cga ctc agc cga atc 192Gly Gln Leu Gly Gln Val Glu Asp Glu Leu Phe Arg Leu Ser Arg Ile
50 55 60ctg gat cgc gaa agc aag ctg act cag ctt ctt tca gat cgc act cag 240Leu Asp Arg Glu Ser Lys Leu Thr Gln Leu Leu Ser Asp Arg Thr Gln65 70 75 80gaa att ggc ggt cga cgt gac ctc ctg gct aag gtg ctc tac ggc aag 288Glu Ile Gly Gly Arg Arg Asp Leu Leu Ala Lys Val Leu Tyr Gly Lys
85 90 95gta act gct gtt acc gaa gcc ctc gca ctg cag gct att ggt cgc cct 336Val Thr Ala Val Thr Glu Ala Leu Ala Leu Gln Ala Ile Gly Arg Pro
100 105 110gag cac aac cca att gac gat atc gca gct ttg gct ggc gct gta gca 384Glu His Asn Pro Ile Asp Asp Ile Ala Ala Leu Ala Gly Ala Val Ala
115 120 125gag cta cag ggt cgt tcc gtt gca cat gtc gtt acc gca gtt gaa ctc 432Glu Leu Gln Gly Arg Ser Val Ala His Val Val Thr Ala Val Glu Leu
130 135 140aac gag gga cag caa caa gcg cta gct gaa aag ctg gga cgt att tat 480Asn Glu Gly Gln Gln Gln Ala Leu Ala Glu Lys Leu Gly Arg Ile Tyr145 150 155 160ggt cgt gcg atg agc atc cac tcc gag gtt gat acc agc ctc ctc ggt 528Gly Arg Ala Met Ser Ile His Ser Glu Val Asp Thr Ser Leu Leu Gly
165 170 175gga atg atc atc cgc gtc gga gac gaa gta att gac ggc agc acc tcg 576Gly Met Ile Ile Arg Val Gly Asp Glu Val Ile Asp Gly Ser Thr Ser
180 185 190ggc aaa ctc gag cgt ctg cgg gca agc ttc gca taaagacacg acgaattaga 629Gly Lys Leu Glu Arg Leu Arg Ala Ser Phe Ala
195 200caa 632<210>772<211>203<212>PRT<213>谷氨酸棒杆菌<400>772Gly Cys Leu Arg Trp Lys Ser Glu Pro Ser Val Leu Glu Val Leu Lys1 5 10 15Asp Ala Ala Glu Gln Thr Trp Ser Thr Pro Arg Glu Phe Arg Ala Gly
20 25 30Leu Val Gln Leu Gly Arg Arg Ala Leu Leu Arg Ser Ala Glu Lys Gln
35 40 45Gly Gln Leu Gly Gln Val Glu Asp Glu Leu Phe Arg Leu Ser Arg Ile
50 55 60Leu Asp Arg Glu Ser Lys Leu Thr Gln Leu Leu Ser Asp Arg Thr Gln65 70 75 80Glu Ile Gly Gly Arg Arg Asp Leu Leu Ala Lys Val Leu Tyr Gly Lys
85 90 95Val Thr Ala Val Thr Glu Ala Leu Ala Leu Gln Ala Ile Gly Arg Pro
100 105 110Glu His Asn Pro Ile Asp Asp Ile Ala Ala Leu Ala Gly Ala Val Ala
115 120 125Glu Leu Gln Gly Arg Ser Val Ala His Val Val Thr Ala Val Glu Leu
130 135 140Asn Glu Gly Gln Gln Gln Ala Leu Ala Glu Lys Leu Gly Arg Ile Tyr145 150 155 160Gly Arg Ala Met Ser Ile His Ser Glu Val Asp Thr Ser Leu Leu Gly
165 170 175Gly Met Ile Ile Arg Val Gly Asp Glu Val Ile Asp Gly Ser Thr Ser
180 185 190Gly Lys Leu Glu Arg Leu Arg Ala Ser Phe Ala
195 200<210>773<211>495<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(472)<223>RXA0l194<400>773acttcgacca ctacccagag caggctttca acggcctcgg tggtttggac gatgtcgaag 60ctgcatacaa gaagctgacc ggaaagtaag gtagagacac atg gct gaa atc acc 115
Met Ala Glu Ile Thr
1 5gtt gaa ctg gtg tct gta gag cgc atg ctg tgg gcc ggc cag gcc tcc 163Val Glu Leu Val Ser Val Glu Arg Met Leu Trp Ala Gly Gln Ala Ser
10 15 20atc gtg act gca cag acc acc gag ggt gag atc ggc gtg ctg ccc gat 211Ile Val Thr Ala Gln Thr Thr Glu Gly Glu Ile Gly Val Leu Pro Asp
25 30 35cac gag cct ctt ctc ggc caa ttg gtt gag aac ggt gtc gtg acc atc 259His Glu Pro Leu Leu Gly Gln Leu Val Glu Asn Gly Val Val Thr Ile
40 45 50cag ccg atc gac ggc gaa aag ctt atc gcc ggc gtt tcg gat gga ttc 307Gln Pro Ile Asp Gly Glu Lys Leu Ile Ala Gly Val Ser Asp Gly Phe
55 60 65ctc tcc gta tct aag gaa aag gtg acg atc ctc gcg gac ttc gcc gtc 355Leu Ser Val Ser Lys Glu Lys Val Thr Ile Leu Ala Asp Phe Ala Val70 75 80 85tgg gcg aat gag gtt gat acc gca tcc gcc gag gct gac ctt aat tcg 403Trp Ala Asn Glu Val Asp Thr Ala Ser Ala Glu Ala Asp Leu Asn Ser
90 95 100gac gac gag ctg gcc aag gca cac gcc gag gct ggg ctg cgc gcg gtc 451Asp Asp Glu Leu Ala Lys Ala His Ala Glu Ala Gly Leu Arg Ala Val
105 110 115cgc cgc agc agc gaa ggt ctc taaacctccg tttagctgaa gta 495Arg Arg Ser Ser Glu Gly Leu
120<210>774<211>124<212>PRT<213>谷氨酸棒杆菌<400>774Met Ala Glu Ile Thr Val Glu Leu Val Ser Val Glu Arg Met Leu Trp1 5 10 15Ala Gly Gln Ala Ser Ile Val Thr Ala Gln Thr Thr Glu Gly Glu Ile
20 25 30Gly Val Leu Pro Asp His Glu Pro Leu Leu Gly Gln Leu Val Glu Asn
35 40 45Gly Val Val Thr Ile Gln Pro Ile Asp Gly Glu Lys Leu Ile Ala Gly
50 55 60Val Ser Asp Gly Phe Leu Ser Val Ser Lys Glu Lys Val Thr Ile Leu65 70 75 80Ala Asp Phe Ala Val Trp Ala Asn Glu Val Asp Thr Ala Ser Ala Glu
85 90 95Ala Asp Leu Asn Ser Asp Asp Glu Leu Ala Lys Ala His Ala Glu Ala
100 105 110Gly Leu Arg Ala Val Arg Arg Ser Ser Glu Gly Leu
115 120<210>775<211>1098<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1075)<223>RXA01202<400>775agaaagacca gctcaccgtt tcccgcaagg tcagcaagaa gtaaggcagc gagcctacac 60taaatgactg tccaagcaac tgaagggagg cgtgtgaacc atg gca aca att cgt 115
Met Ala Thr Ile Arg
1 5gaa ttg cgt gac cga att cgt tcg gtt aac tca acc aag aag atc acc 163Glu Leu Arg Asp Arg Ile Arg Ser Val Asn Ser Thr Lys Lys Ile Thr
10 15 20aag gct caa gag ctc atc gcc acc tct cgc atc acc aag gca cag ggt 211Lys Ala Gln Glu Leu Ile Ala Thr Ser Arg Ile Thr Lys Ala Gln Gly
25 30 35cgc gtc gcg gca gct gcg ccg tac gcc gag gaa atc cag cgc gtg ctg 259Arg Val Ala Ala Ala Ala Pro Tyr Ala Glu Glu Ile Gln Arg Val Leu
40 45 50gag cgc ctc gcg tcg gca agc tcc cta gac cac cca atg ctg cgt gag 307Glu Arg Leu Ala Ser Ala Ser Ser Leu Asp His Pro Met Leu Arg Glu
55 60 65cgt gaa ggc ggc aag cga gcc gcc gtg ctc gtg gtt act tct gac cgc 355Arg Glu Gly Gly Lys Arg Ala Ala Val Leu Val Val Thr Ser Asp Arg70 75 80 85ggc atg gct ggt ggc tac aac cac aac gtt ctg aaa aag gca gcg gag 403Gly Met Ala Gly Gly Tyr Asn His Asn Val Leu Lys Lys Ala Ala Glu
90 95 100ctg gaa aag ctt ctt gct gaa agt gga tac gaa gtg gtt cgt tat gtc 451Leu Glu Lys Leu Leu Ala Glu Ser Gly Tyr Glu Val Val Arg Tyr Val
105 110 115acc ggc aaa aag ggc gtc gac tac tac aag ttc cgc gct gaa gat gtg 499Thr Gly Lys Lys Gly Val Asp Tyr Tyr Lys Phe Arg Ala Glu Asp Val
120 125 130gct ggc acc tgg act gga ttc tca cag gat cca gac tgg gca gct acc 547Ala Gly Thr Trp Thr Gly Phe Ser Gln Asp Pro Asp Trp Ala Ala Thr
135 140 145cac aac gtg cgc cgt cac ctc att gat ggt ttc acc gcc agc tct gaa 595His Asn Val Arg Arg His Leu Ile Asp Gly Phe Thr Ala Ser Ser Glu150 155 160 165ggt gaa gct gca tgg cgc gag gga ctg aac cta cca gaa ggc cag gat 643Gly Glu Ala Ala Trp Arg Glu Gly Leu Asn Leu Pro Glu Gly Gln Asp
170 175 180atc cag ggc ttc gac cag gtt cac gtg gtc tac acc gag ttc atc tcc 691Ile Gln Gly Phe Asp Gln Val His Val Val Tyr Thr Glu Phe Ile Ser
185 190 195atg ctg act caa aac cca gta gtg cac caa ctg ctg cct gtt gag cca 739Met Leu Thr Gln Asn Pro Val Val His Gln Leu Leu Pro Val Glu Pro
200 205 210gtc atc gaa gat gaa att ttc gaa aaa ggc gag gat ctg ctg tcc tct 787Val Ile Glu Asp Glu Ile Phe Glu Lys Gly Glu Asp Leu Leu Ser Ser
215 220 225tcc ggc gaa gtc gaa ccc gac tac gag ttc gag ccg gat gca gac act 835Ser Gly Glu Val Glu Pro Asp Tyr Glu Phe Glu Pro Asp Ala Asp Thr230 235 240 245ctg ctt gag gca ctg ctt ccg cag tac gtc tct cgt agg ctg ttc tcc 883Leu Leu Glu Ala Leu Leu Pro Gln Tyr Val Ser Arg Arg Leu Phe Ser
250 255 260atc ttc ttg gag gct gca gct gca gag tcc gct tca cgt cga aac gcg 931Ile Phe Leu Glu Ala Ala Ala Ala Glu Ser Ala Ser Arg Arg Asn Ala
265 270 275atg aag tct gcg act gac aac gct acg gaa ctg gtc aag gac ctg tcc 979Met Lys Ser Ala Thr Asp Asn Ala Thr Glu Leu Val Lys Asp Leu Ser
280 285 290cgt gtg gcc aac cag gca cgt cag gca cag atc acc cag gaa atc aca 1027Arg Val Ala Asn Gln Ala Arg Gln Ala Gln Ile Thr Gln Glu Ile Thr
295 300 305gag att gtt ggt ggc gca ggc gcg ctc gcc gac agc gga gaa agt gac 1075Glu Ile Val Gly Gly Ala Gly Ala Leu Ala Asp Ser Gly Glu Ser Asp310 315 320 325taattatgac tacagctctt gaa 1098<210>776<211>325<212>PRT<213>谷氨酸棒杆菌<400>776Met Ala Thr Ile Arg Glu Leu Arg Asp Arg Ile Arg Ser Val Asn Ser1 5 10 15Thr Lys Lys Ile Thr Lys Ala Gln Glu Leu Ile Ala Thr Ser Arg Ile
20 25 30Thr Lys Ala Gln Gly Arg Val Ala Ala Ala Ala Pro Tyr Ala Glu Glu
35 40 45Ile Gln Arg Val Leu Glu Arg Leu Ala Ser Ala Ser Ser Leu Asp His
50 55 60Pro Met Leu Arg Glu Arg Glu Gly Gly Lys Arg Ala Ala Val Leu Val65 70 75 80Val Thr Ser Asp Arg Gly Met Ala Gly Gly Tyr Asn His Asn Val Leu
85 90 95Lys Lys Ala Ala Glu Leu Glu Lys Leu Leu Ala Glu Ser Gly Tyr Glu
100 105 110Val Val Arg Tyr Val Thr Gly Lys Lys Gly Val Asp Tyr Tyr Lys Phe
115 120 125Arg Ala Glu Asp Val Ala Gly Thr Trp Thr Gly Phe Ser Gln Asp Pro
130 135 140Asp Trp Ala Ala Thr His Asn Val Arg Arg His Leu Ile Asp Gly Phe145 150 155 160Thr Ala Ser Ser Glu Gly Glu Ala Ala Trp Arg Glu Gly Leu Asn Leu
165 170 175Pro Glu Gly Gln Asp Ile Gln Gly Phe Asp Gln Val His Val Val Tyr
180 185 190Thr Glu Phe Ile Ser Met Leu Thr Gln Asn Pro Val Val His Gln Leu
195 200 205Leu Pro Val Glu Pro Val Ile Glu Asp Glu Ile Phe Glu Lys Gly Glu
210 215 220Asp Leu Leu Ser Ser Ser Gly Glu Val Glu Pro Asp Tyr Glu Phe Glu225 230 235 240Pro Asp Ala Asp Thr Leu Leu Glu Ala Leu Leu Pro Gln Tyr Val Ser
245 250 255Arg Arg Leu Phe Ser Ile Phe Leu Glu Ala Ala Ala Ala Glu Ser Ala
260 265 270Ser Arg Arg Asn Ala Met Lys Ser Ala Thr Asp Asn Ala Thr Glu Leu
275 280 285Val Lys Asp Leu Ser Arg Val Ala Asn Gln Ala Arg Gln Ala Gln Ile
290 295 300Thr Gln Glu Ile Thr Glu Ile Val Gly Gly Ala Gly Ala Leu Ala Asp305 310 315 320Ser Glu Ser Asp
325<210>777<211>1773<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101).. (1750)<223>RXN02434<400>777cttcaatagt caaaaccagc aaactaattt tttaagtttt acgtaactgg ccccaccgct 60tgtggcaggc cttgcgtttt gacattgaag gacccttttt atg cgc act ttt gcc 115
Met Arg Thr Phe Ala
1 5gct tat att gcc att gat ggc ctc agc ttt tcc tac ccc aac acc cac 163Ala Tyr Ile Ala Ile Asp Gly Leu Ser Phe Ser Tyr Pro Asn Thr His
10 15 20gtt tta agc gat att tcg ctc acc gtt gcc aat ggc gat atc gcc gga 211Val Leu Ser Asp Ile Ser Leu Thr Val Ala Asn Gly Asp Ile Ala Gly
25 30 35ctg att ggt gaa aac ggc gca gga aaa tcc acc ctg ctc agc ctc atc 259Leu Ile Gly Glu Asn Gly Ala Gly Lys Ser Thr Leu Leu Ser Leu Ile
40 45 50gct ggc gtc atg gaa ccc gac cag ggc agg att tac ctc ccc gaa cgc 307Ala Gly Val Met Glu Pro Asp Gln Gly Arg Ile Tyr Leu Pro Glu Arg
55 60 65acc gga ttc atc gcc caa gaa aca gac tta ccg ttt gaa caa ccc gtg 355Thr Gly Phe Ile Ala Gln Glu Thr Asp Leu Pro Phe Glu Gln Pro Val70 75 80 85cag tcg ctt atc gac gcc gcc gtc gcc cca gtg cgc gcg gtc gat gcc 403Gln Ser Leu Ile Asp Ala Ala Val Ala Pro Val Arg Ala Val Asp Ala
90 95 100gcg att aca gat ttg tcc acc aag ctt ggc gac gcc tcc ctc agc gcc 451Ala Ile Thr Asp Leu Ser Thr Lys Leu Gly Asp Ala Ser Leu Ser Ala
105 110 115gaa gag cag gcg caa gtc gcc aca gat ttc gat gca gcg cta ggc gct 499Glu Glu Gln Ala Gln Val Ala Thr Asp Phe Asp Ala Ala Leu Gly Ala
120 125 130gca gaa gaa ctc gga ctg tgg gaa tta gat gca cgt att gaa acc atc 547Ala Glu Glu Leu Gly Leu Trp Glu Leu Asp Ala Arg Ile Glu Thr Ile
135 140 145gtc gcg ggt ctc ggc ctt gcc gag gtg gat cgc agc act ccc att ggt 595Val Ala Gly Leu Gly Leu Ala Glu Val Asp Arg Ser Thr Pro Ile Gly150 155 160 165gag ctt tcc ggc ggt cag cgc cgc aga ttc gca ttg gca gcg ctg ctg 643Glu Leu Ser Gly Gly Gln Arg Arg Arg Phe Ala Leu Ala Ala Leu Leu
170 175 180ttg gaa cca cac gat gct ctg att ttc gat gag ccc acc aac cac ctc 691Leu Glu Pro His Asp Ala Leu Ile Phe Asp Glu Pro Thr Asn His Leu
185 190 195gac gac aca gcc gta gat ttc ctc atc tcg gag att tcc cgt ttc aaa 739Asp Asp Thr Ala Val Asp Phe Leu Ile Ser Glu Ile Ser Arg Phe Lys
200 205 210ggt cca gtg ctg atc gcc agc cac gat cgc ttc ttc ctc gac tcc gtc 787Gly Pro Val Leu Ile Ala Ser His Asp Arg Phe Phe Leu Asp Ser Val
215 220 225tgt acc gag tta atc gac ctc gat cct gca ctt gga cct gag ggc gga 835Cys Thr Glu Leu Ile Asp Leu Asp Pro Ala Leu Gly Pro Glu Gly Gly230 235 240 245tcc ggc gaa gaa gta aaa caa gcc gtg tct ttt ggt ggt gga ttt tct 883Ser Gly Glu Glu Val Lys Gln Ala Val Ser Phe Gly Gly Gly Phe Ser
250 255 260gaa tac atc aaa gaa cgc gag acc cgc cgc acc cgc tgg gct cag ttg 931Glu Tyr Ile Lys Glu Arg Glu Thr Arg Arg Thr Arg Trp Ala Gln Leu
265 270 275tac acc gca caa gaa acc gag cgg gaa aaa ctc gaa gaa acc acc ggc 979Tyr Thr Ala Gln Glu Thr Glu Arg Glu Lys Leu Glu Glu Thr Thr Gly
280 285 290acc acc gaa tcg gat att ttc cac agc tcg gtt tcc aaa tcg gaa gct 1027Thr Thr Glu Ser Asp Ile Phe His Ser Ser Val Ser Lys Ser Glu Ala
295 300 305aaa atc acc gcg aaa ttt tac gca gac cgg gca gct aaa act caa ggc 1075Lys Ile Thr Ala Lys Phe Tyr Ala Asp Arg Ala Ala Lys Thr Gln Gly310 315 320 325aac cgc gtc cgc tcc gcc aaa aac cgc ctg aag gaa ttg gaa cgc tat 1123Asn Arg Val Arg Ser Ala Lys Asn Arg Leu Lys Glu Leu Glu Arg Tyr
330 335 340gaa atc cca gca cct cca aag cca ctg gaa ttc caa ggc atc cca gaa 1171Glu Ile Pro Ala Pro Pro Lys Pro Leu Glu Phe Gln Gly Ile Pro Glu
345 350 355gcc tcc gga aac ggt cac ggt gaa aca cta gaa gtg cgg gct att gct 1219Ala Ser Gly Asn Gly His Gly Glu Thr Leu Glu Val Arg Ala Ile Ala
360 365 370gtg gaa aac agg ctt caa ccc ttg act ttc cac atc gat ccc ggc gac 1267Val Glu Asn Arg Leu Gln Pro Leu Thr Phe His Ile Asp Pro Gly Asp
375 380 385cac atc ctg gtc gaa ggc ccc aac ggt gtc ggt aaa tcc acc ctg ctg 1315His Ile Leu Val Glu Gly Pro Asn Gly Val Gly Lys Ser Thr Leu Leu390 395 400 405agc gtt ctg gaa ggc gtg ctt gaa cca acc gaa ggt gaa ttg atc gtc 1363Ser Val Leu Glu Gly Val Leu Glu Pro Thr Glu Gly Glu Leu Ile Val
410 415 420ccc gaa ggg ctg aaa gtt gcg cgc ctg aaa cag gac gat cag tgg acg 1411Pro Glu Gly Leu Lys Val Ala Arg Leu Lys Gln Asp Asp Gln Trp Thr
425 430 435gaa aag cag ttg aac acc ccc gtc gac gaa ctg ttc gcc gcc cta tcg 1459Glu Lys Gln Leu Asn Thr Pro Val Asp Glu Leu Phe Ala Ala Leu Ser
440 445 450aaa ggt ccg gtc gga ctc aac ctc gtg gag atg ggg ctg ttg agg gag 1507Lys Gly Pro Val Gly Leu Asn Leu Val Glu Met Gly Leu Leu Arg Glu
455 460 465acg tcg caa agc agc ccg cta cgg gcc cta tcg ctc ggc caa cgc cgg 1555Thr Ser Gln Ser Ser Pro Leu Arg Ala Leu Ser Leu Gly Gln Arg Arg470 475 480 485cgc gtc tcg ctc ggg ctc atc ctg gcg agc cca cca gat ctt ttg ctt 1603Arg Val Ser Leu Gly Leu Ile Leu Ala Ser Pro Pro Asp Leu Leu Leu
490 495 500ctt gac gag ccc acc aac cac ctc tcc ctc gcg ctg agc gaa gaa ctc 1651Leu Asp Glu Pro Thr Asn His Leu Ser Leu Ala Leu Ser Glu Glu Leu
505 510 515gag tcg gcg ata gaa aaa ttc ccc ggt cgc gtt att ctg gcc agc cac 1699Glu Ser Ala Ile Glu Lys Phe Pro Gly Arg Val Ile Leu Ala Ser His
520 525 530gat agg tgg atc aga aaa cgt tgg acg ggg aag aaa atc agc ctg agc 1747Asp Arg Trp Ile Arg Lys Arg Trp Thr Gly Lys Lys Ile Ser Leu Ser
535 540 545cgt taaaccctac tgaacaggaa cct 1773Arg550<210>778<211>550<212>PRT<213>谷氨酸棒杆菌<400>778Met Arg Thr Phe Ala Ala Tyr Ile Ala Ile Asp Gly Leu Ser Phe Ser1 5 10 15Tyr Pro Asn Thr His Val Leu Ser Asp Ile Ser Leu Thr Val Ala Asn
20 25 30Gly Asp Ile Ala Gly Leu Ile Gly Glu Asn Gly Ala Gly Lys Ser Thr
35 40 45Leu Leu Ser Leu Ile Ala Gly Val Met Glu Pro Asp Gln Gly Arg Ile
50 55 60Tyr Leu Pro Glu Arg Thr Gly Phe Ile Ala Gln Glu Thr Asp Leu Pro65 70 75 80Phe Glu Gln Pro Val Gln Ser Leu Ile Asp Ala Ala Val Ala Pro Val
85 90 95Arg Ala Val Asp Ala Ala Ile Thr Asp Leu Ser Thr Lys Leu Gly Asp
100 105 110Ala Ser Leu Ser Ala Glu Glu Gln Ala Gln Val Ala Thr Asp Phe Asp
115 120 125Ala Ala Leu Gly Ala Ala Glu Glu Leu Gly Leu Trp Glu Leu Asp Ala
130 135 140Arg Ile Glu Thr Ile Val Ala Gly Leu Gly Leu Ala Glu Val Asp Arg145 150 155 160Ser Thr Pro Ile Gly Glu Leu Ser Gly Gly Gln Arg Arg Arg Phe Ala
165 170 175Leu Ala Ala Leu Leu Leu Glu Pro His Asp Ala Leu Ile Phe Asp Glu
180 185 190Pro Thr Asn His Leu Asp Asp Thr Ala Val Asp Phe Leu Ile Ser Glu
195 200 205Ile Ser Arg Phe Lys Gly Pro Val Leu Ile Ala Ser His Asp Arg Phe
210 215 220Phe Leu Asp Ser Val Cys Thr Glu Leu Ile Asp Leu Asp Pro Ala Leu225 230 235 240Gly Pro Glu Gly Gly Ser Gly Glu Glu Val Lys Gln Ala Val Ser Phe
245 250 255Gly Gly Gly Phe Ser Glu Tyr Ile Lys Glu Arg Glu Thr Arg Arg Thr
260 265 270Arg Trp Ala Gln Leu Tyr Thr Ala Gln Glu Thr Glu Arg Glu Lys Leu
275 280 285Glu Glu Thr Thr Gly Thr Thr Glu Ser Asp Ile Phe His Ser Ser Val
290 295 300Ser Lys Ser Glu Ala Lys Ile Thr Ala Lys Phe Tyr Ala Asp Arg Ala305 310 315 320Ala Lys Thr Gln Gly Asn Arg Val Arg Ser Ala Lys Asn Arg Leu Lys
325 330 335Glu Leu Glu Arg Tyr Glu Ile Pro Ala Pro Pro Lys Pro Leu Glu Phe
340 345 350Gln Gly Ile Pro Glu Ala Ser Gly Asn Gly His Gly Glu Thr Leu Glu
355 360 365Val Arg Ala Ile Ala Val Glu Asn Arg Leu Gln Pro Leu Thr Phe His
370 375 380Ile Asp Pro Gly Asp His Ile Leu Val Glu Gly Pro Asn Gly Val Gly385 390 395 400Lys Ser Thr Leu Leu Ser Val Leu Glu Gly Val Leu Glu Pro Thr Glu
405 410 415Gly Glu Leu Ile Val Pro Glu Gly Leu Lys Val Ala Arg Leu Lys Gln
420 425 430Asp Asp Gln Trp Thr Glu Lys Gln Leu Asn Thr Pro Val Asp Glu Leu
435 440 445Phe Ala Ala Leu Ser Lys Gly Pro Val Gly Leu Asn Leu Val Glu Met
450 455 460Gly Leu Leu Arg Glu Thr Ser Gln Ser Ser Pro Leu Arg Ala Leu Ser465 470 475 480Leu Gly Gln Arg Arg Arg Val Ser Leu Gly Leu Ile Leu Ala Ser Pro
485 490 495Pro Asp Leu Leu Leu Leu Asp Glu Pro Thr Asn His Leu Ser Leu Ala
500 505 510Leu Ser Glu Glu Leu Glu Ser Ala Ile Glu Lys Phe Pro Gly Arg Val
515 520 525Ile Leu Ala Ser His Asp Arg Trp Ile Arg Lys Arg Trp Thr Gly Lys
530 535 540Lys Ile Ser Leu Ser Arg545 550<210>779<211>1407<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(1384)<223>RXN00684<400>779agtcacacct aaaagtgata gccatcacga atctttagga aaagtgattc aaacttcact 60gtgatcggct tcggccacac acaagtgtca ggagatgaca atg act tcc cag act 115
Met Thr Ser Gln Thr
1 5tcc caa caa tcc acc tca acc ggt gga tgc cca ttc ggg cac aca tca 163Ser Gln Gln Ser Thr Ser Thr Gly Gly Cys Pro Phe Gly His Thr Ser
10 15 20gag tcc acc agc cat cac ggc tac cag cct ttc gat atg cac aac ccg 211Glu Ser Thr Ser His His Gly Tyr Gln Pro Phe Asp Met His Asn Pro
25 30 35ttt cct gca tat aaa gaa ctc cgt cag gaa gag cca gtg atg ttc gat 259Phe Pro Ala Tyr Lys Glu Leu Arg Gln Glu Glu Pro Val Met Phe Asp
40 45 50gag cgc atc ggc tac tgg gtg gta acc aaa tat gac gac atc aaa acc 307Glu Arg Ile Gly Tyr Trp Val Val Thr Lys Tyr Asp Asp Ile Lys Thr
55 60 65acc ttt gat gac tgg gaa aca ttc tcc tct gaa aat gca caa gcc cca 355Thr Phe Asp Asp Trp Glu Thr Phe Ser Ser Glu Asn Ala Gln Ala Pro70 75 80 85gtc cgc aag cgt gga cct cag gca acc caa atc atg acc gat ggc ggc 403Val Arg Lys Arg Gly Pro Gln Ala Thr Gln Ile Met Thr Asp Gly Gly
90 95 100ttc act gca tac tcc gga tta tca gct cgt att cca cca gag cac acc 451Phe Thr Ala Tyr Ser Gly Leu Ser Ala Arg Ile Pro Pro Glu His Thr
105 110 115cgc atc cgc gca atc gca caa aag gcc ttc acg cca cgc cgc tat aaa 499Arg Ile Arg Ala Ile Ala Gln Lys Ala Phe Thr Pro Arg Arg Tyr Lys
120 125 130gca ctc gaa cca gat atc cga gca atg gtg att gat cgt gtg gag aaa 547Ala Leu Glu Pro Asp Ile Arg Ala Met Val Ile Asp Arg Val Glu Lys
135 140 145atg ttg gcg aat gat caa cac gtc ggc gat atg gtg tca gat ctt gcc 595Met Leu Ala Asn Asp Gln His Val Gly Asp Met Val Ser Asp Leu Ala150 155 160 165tac gac att cca acc atc acg atc ctg acg ctg atc ggt gca gat att 643Tyr Asp Ile Pro Thr Ile Thr Ile Leu Thr Leu Ile Gly Ala Asp Ile
170 175 180ttc atg gtg gtc acc tac aag cgg tgg tca gat tcc cgt gcg gcc atg 691Phe Met Val Val Thr Tyr Lys Arg Trp Ser Asp Ser Arg Ala Ala Met
185 190 195acc tgg ggc gat ctt agt gat gaa gag cag atc cca cac gca cac aat 739Thr Trp Gly Asp Leu Ser Asp Glu Glu Gln Ile Pro His Ala His Asn
200 205 210ttg gtt gag tac tgg cag gaa tgc caa cgc atg gta gct gat gca cat 787Leu Val Glu Tyr Trp Gln Glu Cys Gln Arg Met Val Ala Asp Ala His
215 220 225gca cac ggt ggc gac aac ctc acc gct gat cta gtg cga gca cag caa 835Ala His Gly Gly Asp Asn Leu Thr Ala Asp Leu Val Arg Ala Gln Gln230 235 240 245gag ggt caa gaa atc acc gat cat gag att gct tct ttg ctg tac tcc 883Glu Gly Gln Glu Ile Thr Asp His Glu Ile Ala Ser Leu Leu Tyr Ser
250 255 260ctg ctt ttt gcg ggg cac gaa aca acc acc acg ttg atc tcc aat tgt 931Leu Leu Phe Ala Gly His Glu Thr Thr Thr Thr Leu Ile Ser Asn Cys
265 270 275ttc cga gtt ctc ctc gat cat cca gag cag tgg caa gcc att cta gag 979Phe Arg Val Leu Leu Asp His Pro Glu Gln Trp Gln Ala Ile Leu Glu
280 285 290aat cca aaa ctg att cct gcg gca gtg gat gag gtc ttg cgg tac tcc 1027Asn Pro Lys Leu Ile Pro Ala Ala Val Asp Glu Val Leu Arg Tyr Ser
295 300 305ggc tcg atc gtg ggg tgg cgt cga aaa gca tta aaa gac acc gag atc 1075Gly Ser Ile Val Gly Trp Arg Arg Lys Ala Leu Lys Asp Thr Glu Ile310 315 320 325ggc ggc gtt gcc att aag gaa ggc gat ggt gtt ctg ctg ctc atg ggt 1123Gly Gly Val Ala Ile Lys Glu Gly Asp Gly Val Leu Leu Leu Met Gly
330 335 340tcc gcg aac cgc gat gaa gct cgc ttt gaa aat ggc gag gaa ttc gat 1171Ser Ala Asn Arg Asp Glu Ala Arg Phe Glu Asn Gly Glu Glu Phe Asp
345 350 355atc agc cgc gct aat gcg cgc gag cac ctg tct ttt ggt ttc ggc atc 1219Ile Ser Arg Ala Asn Ala Arg Glu His Leu Ser Phe Gly Phe Gly Ile
360 365 370cac tat tgc cta gga aac atg ctg gcc aaa ctt caa gcc aag atc tgt 1267His Tyr Cys Leu Gly Asn Met Leu Ala Lys Leu Gln Ala Lys Ile Cys
375 380 385ctc gag gaa gtc acc agg ctt gtt cct tcc ctg cac ttg gtt gcg gac 1315Leu Glu Glu Val Thr Arg Leu Val Pro Ser Leu His Leu Val Ala Asp390 395 400 405aaa gct atc ggg ttc cgg gag aac ctc tcc ttc cgc gtc ccc act tct 1363Lys Ala Ile Gly Phe Arg Glu Asn Leu Ser Phe Arg Val Pro Thr Ser
410 415 420gtt ccc gtg act tgg aac gct taacgcttta ttaaataagg aga 1407Val Pro Val Thr Trp Asn Ala
425<210>780<211>428<212>PRT<213>谷氨酸棒杆菌<400>780Met Thr Ser Gln Thr Ser Gln Gln Ser Thr Ser Thr Gly Gly Cys Pro1 5 10 15Phe Gly His Thr Ser Glu Ser Thr Ser His His Gly Tyr Gln Pro Phe
20 25 30Asp Met His Asn Pro Phe Pro Ala Tyr Lys Glu Leu Arg Gln Glu Glu
35 40 45Pro Val Met Phe Asp Glu Arg Ile Gly Tyr Trp Val Val Thr Lys Tyr
50 55 60Asp Asp Ile Lys Thr Thr Phe Asp Asp Trp Glu Thr Phe Ser Ser Glu65 70 75 80Asn Ala Gln Ala Pro Val Arg Lys Arg Gly Pro Gln Ala Thr Gln Ile
85 90 95Met Thr Asp Gly Gly Phe Thr Ala Tyr Ser Gly Leu Ser Ala Arg Ile
100 105 110Pro Pro Glu His Thr Arg Ile Arg Ala Ile Ala Gln Lys Ala Phe Thr
115 120 125Pro Arg Arg Tyr Lys Ala Leu Glu Pro Asp Ile Arg Ala Met Val Ile
130 135 140Asp Arg Val Glu Lys Met Leu Ala Asn Asp Gln His Val Gly Asp Met145 150 155 160Val Ser Asp Leu Ala Tyr Asp Ile Pro Thr Ile Thr Ile Leu Thr Leu
165 170 175Ile Gly Ala Asp Ile Phe Met Val Val Thr Tyr Lys Arg Trp Ser Asp
180 185 190Ser Arg Ala Ala Met Thr Trp Gly Asp Leu Ser Asp Glu Glu Gln Ile
195 200 205Pro His Ala His Asn Leu Val Glu Tyr Trp Gln Glu Cys Gln Arg Met
210 215 220Val Ala Asp Ala His Ala His Gly Gly Asp Asn Leu Thr Ala Asp Leu225 230 235 240Val Arg Ala Gln Gln Glu Gly Gln Glu Ile Thr Asp His Glu Ile Ala
245 250 255Ser Leu Leu Tyr Ser Leu Leu Phe Ala Gly His Glu Thr Thr Thr Thr
260 265 270Leu Ile Ser Asn Cys Phe Arg Val Leu Leu Asp His Pro Glu Gln Trp
275 280 285Gln Ala Ile Leu Glu Asn Pro Lys Leu Ile Pro Ala Ala Val Asp Glu
290 295 300Val Leu Arg Tyr Ser Gly Ser Ile Val Gly Trp Arg Arg Lys Ala Leu305 310 315 320Lys Asp Thr Glu Ile Gly Gly Val Ala Ile Lys Glu Gly Asp Gly Val
325 330 335Leu Leu Leu Met Gly Ser Ala Asn Arg Asp Glu Ala Arg Phe Glu Asn
340 345 350Gly Glu Glu Phe Asp Ile Ser Arg Ala Asn Ala Arg Glu His Leu Ser
355 360 365Phe Gly Phe Gly Ile His Tyr Cys Leu Gly Asn Met Leu Ala Lys Leu
370 375 380Gln Ala Lys Ile Cys Leu Glu Glu Val Thr Arg Leu Val Pro Ser Leu385 390 395 400His Leu Val Ala Asp Lys Ala Ile Gly Phe Arg Glu Asn Leu Ser Phe
405 410 415Arg Val Pro Thr Ser Val Pro Val Thr Trp Asn Ala
420 425<210>781<211>978<212>DNA<213>谷氨酸棒杆菌<220><221>CDS<222>(101)..(955)<223>RXN00387<400>781ttccgcgccg gcgccaactt cgacggcacc gggctgacca ccttctgctt tgaagcgcac 60gatttctccg ccgactacct cgccaacggg ccaggccgag atg ttc cgc tcg aat 115
Met Phe Arg Ser Asn
1 5att tcc tac gca gtc ggc gac gac atc caa aac gac cca gaa acc tgg 163Ile Ser Tyr Ala Val Gly Asp Asp Ile Gln Asn Asp Pro Glu Thr Trp
10 15 20gaa gac tac gaa ctt cgc gtc aac cac cca ctg cgc atc gaa ggc gac 211Glu Asp Tyr Glu Leu Arg Val Asn His Pro Leu Arg Ile Glu Gly Asp
25 30 35cgc gtc tac ctt cag ggc cac ggc ttc gcc cca aca ttc acc gtg acc 259Arg Val Tyr Leu Gln Gly His Gly Phe Ala Pro Thr Phe Thr Val Thr
40 45 50tgg cca aat ggc gag acc cgc acc cag acc gtg cag tgg cgc cca gac 307Trp Pro Asn Gly Glu Thr Arg Thr Gln Thr Val Gln Trp Arg Pro Asp
55 60 65gac ccg acc ttc ttc ctg tcc tca ggc gtg gtc cgt ttc gat cca ccc 355Asp Pro Thr Phe Phe Leu Ser Ser Gly Val Val Arg Phe Asp Pro Pro70 75 80 85gcc ggc atg tac cca gac ctt tac gag cgc cgc caa aac cag ttg gcc 403Ala Gly Met Tyr Pro Asp Leu Tyr Glu Arg Arg Gln Asn Gln Leu Ala
90 95 100atc cag gga ctt ttc gca ccg acc gcg gaa tgg gaa ggc gac aac aac 451Ile Gln Gly Leu Phe Ala Pro Thr Ala Glu Trp Glu Gly Asp Asn Asn
105 110 115gaa ctg ctg acc tcc tcc tac ccg gcg atg cgt gac cca gcc gtg gcg 499Glu Leu Leu Thr Ser Ser Tyr Pro Ala Met Arg Asp Pro Ala Val Ala
120 125 130atc gat att tac cgc ggc gac aat ggc ctc gat acc ggc atc gga cag 547Ile Asp Ile Tyr Arg Gly Asp Asn Gly Leu Asp Thr Gly Ile Gly Gln
135 140 145tca ttg ttc agc ctg gac tct agt ctc atg cac agc ggc gtg ctg caa 595Ser Leu Phe Ser Leu Asp Ser Ser Leu Met His Ser Gly Val Leu Gln150 155 160 165aaa att gag cgc gtc aac ctc caa atc ggc gac acc gtc acc ctg gat 643Lys Ile Glu Arg Val Asn Leu Gln Ile Gly Asp Thr Val Thr Leu Asp
170 175 180gat ggc acc acc gtc tcc ttc gac ggc gcg tca gaa ttt gcc aac tac 691Asp Gly Thr Thr Val Ser Phe Asp Gly Ala Ser Glu Phe Ala Asn Tyr
185 190 195cag atc agc cgc gac ccc aca caa aac tgg gtg ctg gtc acc acc gtg 739Gln Ile Ser Arg Asp Pro Thr Gln Asn Trp Val Leu Val Thr Thr Val
200 205 210att tcg ctg gtc tcc ctg gtt gga tcc ctg atg atc cga cgc cgc cgc 787Ile Ser Leu Val Ser Leu Val Gly Ser Leu Met Ile Arg Arg Arg Arg
215 220 225att tgg gtg cgt ttc tat cca caa gaa aac gga acc acc cgc gtg gaa 835Ile Trp Val Arg Phe Tyr Pro Gln Glu Asn Gly Thr Thr Arg Val Glu230 235 240 245acc ggc gga ctt gcc cgc acc gac cgc gca ggc tgg ggt ggc gaa tac 883Thr Gly Gly Leu Ala Arg Thr Asp Arg Ala Gly Trp Gly Gly Glu Tyr
250 255 260gag aaa ttc cac cgc gaa ctg ctg ggt ctg aag gag gaa gat gaa gac 931Glu Lys Phe His Arg Glu Leu Leu Gly Leu Lys Glu Glu Asp Glu Asp
265 270 275gaa gag tac ttc gac cac gac gac taacaccgca atttaaaggc ttt 978Glu Glu Tyr Phe Asp His Asp Asp
280 285<210>782<211>285<212>PRT<213>谷氨酸棒杆菌<400>782Met Phe Arg Ser Asn Ile Ser Tyr Ala Val Gly Asp Asp Ile Gln Asn1 5 10 15Asp Pro Glu Thr Trp Glu Asp Tyr Glu Leu Arg Val Asn His Pro Leu
20 25 30Arg Ile Glu Gly Asp Arg Val Tyr Leu Gln Gly His Gly Phe Ala Pro
35 40 45Thr Phe Thr Val Thr Trp Pro Asn Gly Glu Thr Arg Thr Gln Thr Val
50 55 60Gln Trp Arg Pro Asp Asp Pro Thr Phe Phe Leu Ser Ser Gly Val Val65 70 75 80Arg Phe Asp Pro Pro Ala Gly Met Tyr Pro Asp Leu Tyr Glu Arg Arg
85 90 95Gln Asn Gln Leu Ala Ile Gln Gly Leu Phe Ala Pro Thr Ala Glu Trp
100 105 110Glu Gly Asp Asn Asn Glu Leu Leu Thr Ser Ser Tyr Pro Ala Met Arg
115 120 125Asp Pro Ala Val Ala Ile Asp Ile Tyr Arg Gly Asp Asn Gly Leu Asp
130 135 140Thr Gly Ile Gly Gln Ser Leu Phe Ser Leu Asp Ser Ser Leu Met His145 150 155 160Ser Gly Val Leu Gln Lys Ile Glu Arg Val Asn Leu Gln Ile Gly Asp
165 170 175Thr Val Thr Leu Asp Asp Gly Thr Thr Val Ser Phe Asp Gly Ala Ser
180 185 190Glu Phe Ala Asn Tyr Gln Ile Ser Arg Asp Pro Thr Gln Asn Trp Val
195 200 205Leu Val Thr Thr Val Ile Ser Leu Val Ser Leu Val Gly Ser Leu Met
210 215 220Ile Arg Arg Arg Arg Ile Trp Val Arg Phe Tyr Pro Gln Glu Asn Gly225 230 235 240Thr Thr Arg Val Glu Thr Gly Gly Leu Ala Arg Thr Asp Arg Ala Gly
245 250 255Trp Gly Gly Glu Tyr Glu Lys Phe His Arg Glu Leu Leu Gly Leu Lys
260 265 270Glu Glu Asp Glu Asp Glu Glu Tyr Phe Asp His Asp Asp
275 280 285
Claims (38)
1.一种分离的编码SMP蛋白的谷氨酸棒杆菌核酸分子或其部分,前提是所述核酸分子不是由表1给出的任何F标志基因组成。
2.权利要求1的分离核酸分子,其中所述核酸分子编码参与产生精细化学品的SMP蛋白。
3.一种分离的谷氨酸棒杆菌核酸分子或其部分,所述核酸分子选自序列表奇数SEQ ID NO序列,前提是所述核酸分子不是由表1给出的任何F标志基因组成。
4.一种分离的核酸分子,所述核酸分子编码选自序列表偶数SEQID NO序列的多肽序列,前提是所述核酸分子不是由表1给出的任何F标志基因组成。
5.一种分离的核酸分子,它编码一种多肽的天然等位基因变异体,所述多肽选自序列表偶数SEQ ID NO序列组成的氨基酸序列,前提是所述核酸分子不是由表1给出的任何F标志基因组成。
6.一种分离的核酸分子或其部分,所述核酸分子包含与选自序列表奇数SEQ ID NO序列的核苷酸序列具有至少50%同源性的核苷酸序列,前提是所述核酸分子不是由表1给出的任何F标志基因组成。
7.一种分离的核酸分子,它包含一种核酸的至少15个核苷酸的片段,所述核酸包含选自序列表奇数SEQ ID NO序列的核苷酸序列,前提是所述核酸分子不是由表1给出的任何F标志基因组成。
8.一种分离的核酸分子,它在严格条件下与权利要求1-7任一项的核酸分子杂交。
9.一种分离的核酸分子,该核酸分子包括权利要求1-8任一项的核酸分子或其部分和编码异源多肽的核苷酸序列。
10.一种载体,它包含权利要求1-9任一项的核酸分子。
11.权利要求10的载体,它为一种表达载体。
12.一种宿主细胞,它是用权利要求11的表达载体转染的宿主细胞。
13.权利要求12的宿主细胞,其中所述细胞是一种微生物。
14.权利要求13的宿主细胞,其中所述细胞属于棒杆菌属(Corynebacterium)或短杆菌属(Brevibacterium)。
15.权利要求12的宿主细胞,其中所述核酸分子表达对所述细胞产生精细化学品产生调节。
16.权利要求15的宿主细胞,其中所述精细化学品选自:有机酸、形成蛋白质和非形成蛋白质的氨基酸、嘌呤和嘧啶碱基、核苷、核苷酸、脂质、饱和和非饱和脂肪酸、二元醇、碳水化合物、芳香族化合物、维生素、辅因子、聚酮化合物和酶。
17.一种产生多肽的方法,该方法包括在合适培养基中培养权利要求12的宿主细胞,由此产生所述多肽。
18.一种分离的谷氨酸棒杆菌SMP多肽或其部分。
19.权利要求18的多肽,其中所述多肽参与产生精细化学品。
20.一种分离的多肽,它包含选自序列表偶数SEQ ID NO序列的氨基酸序列,前提是所述氨基酸序列不是由表1给出的任何F标志基因编码。
21.一种分离的多肽或其部分,它包括一种多肽的天然等位基因变异体,所述多肽包含选自序列表偶数SEQ ID NO序列的氨基酸序列,前提是所述氨基酸序列不是由表1给出的任何F标志基因编码。
22.权利要求18-21任一项的分离多肽,它还包括异源氨基酸序列。
23.一种分离的多肽,它由一种核酸分子编码,所述核酸分子包含与选自序列表奇数SEQ ID NO序列的核酸具有至少50%同源性的核苷酸序列,前提是所述核酸分子不是由表1给出的任何F标志核酸分子组成。
24.一种分离的多肽,它包含与选自序列表偶数SEQ ID NO序列的氨基酸序列具有至少50%同源性的氨基酸序列,前提是所述氨基酸序列不是由表1给出的任何F标志基因编码。
25.一种产生精细化学品的方法,该方法包括培养含有权利要求12的载体的细胞,由此产生所述精细化学品。
26.权利要求25的方法,其中所述方法还包括从所述培养物回收所述精细化学品的步骤。
27.权利要求25的方法,其中所述方法还包括用权利要求11的载体转染所述细胞,获得含有所述载体的细胞的步骤。
28.权利要求25的方法,其中所述细胞属于棒杆菌属或短杆菌属。
29.权利要求25的方法,其中所述细胞选自:谷氨酸棒杆菌(Corynebacterium glutamicum)、力士棒杆菌(Corynebacteriumherculis)、百合花棒杆菌(Corynebacterium lilium)、嗜乙酰乙酸棒杆菌(Corynebacterium acetoacidophilum)、醋谷棒杆菌(Corynebacteriumacetoglutamicum)、嗜乙酰棒杆菌(Corynebacterium acetophilum)、产氨棒杆菌(Corynebacterium ammoniagenes)、Corynebacterium fujiokense、Corynebacterium nitrilophilus、产氨短杆菌(Brevibacteriumammoniagenes)、Brevibacterium butanicum、谷氨酸短杆菌(Brevibacterium divaricatum)、黄色短杆菌(Brevibacterium flavum)、希氏短杆菌(Brevibacterium healii)、酮戊二酸短杆菌(Brevibacteriumketoglutamicum)、Brevibacterium ketosoreductum、乳发酵短杆菌(Brevibacterium lactofermentum)、扩展短杆菌(Brevibacterium linens)、Brevibacterium paraffinolyticum和表3给出的菌株。
30.权利要求25的方法,其中由所述载体表达所述核酸分子使得对所述精细化学品的产生进行调节。
31.权利要求25的方法,其中所述精细化学品选自:有机酸、形成蛋白质和非形成蛋白质的氨基酸、嘌呤和嘧啶碱基、核苷、核苷酸、脂质、饱和和非饱和脂肪酸、二元醇、碳水化合物、芳香族化合物、维生素、辅因子、聚酮化合物和酶。
32.权利要求25的方法,其中所述精细化学品为氨基酸。
33.权利要求32的方法,其中所述氨基酸选自:赖氨酸、谷氨酸、谷氨酰胺、丙氨酸、天冬氨酸、甘氨酸、丝氨酸、苏氨酸、甲硫氨酸、半胱氨酸、缬氨酸、亮氨酸、异亮氨酸、精氨酸、脯氨酸、组氨酸、酪氨酸、苯丙氨酸和色氨酸。
34.一种产生精细化学品的方法,该方法包括培养其基因组DNA因为含有权利要求1-9任一项的核酸分子而改变的细胞。
35.一种诊断受试者体内是否存在白喉棒杆菌或其活动情况的方法,该方法包括检测所述受试者体内是否存一种或多种序列表SEQ IDNO 1-782序列,前提是所述序列不是表1给出的任何F标志序列或由其编码,由此诊断所述受试者体内是否存在白喉棒杆菌或其活动情况。
36.一种宿主细胞,它包含选自序列表奇数SEQ ID NO核酸分子的核酸分子,其中所述核酸分子被破坏。
37.一种宿主细胞,它包含选自序列表奇数SEQ ID NO核酸分子的核酸分子,其中所述核酸分子相对于序列表奇数SEQ ID NO序列存在一个或多个核酸修饰。
38.一种宿主细胞,它包含选自序列表奇数SEQ ID NO核酸分子的核酸分子,其中相对于所述核酸分子的野生型调节区,修饰了所述核酸分子的调节区。
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CN111500566A (zh) * | 2019-01-28 | 2020-08-07 | 江南大学 | 一种海藻糖合成酶突变体及其制备方法和应用 |
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CN111500566A (zh) * | 2019-01-28 | 2020-08-07 | 江南大学 | 一种海藻糖合成酶突变体及其制备方法和应用 |
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