AU2006200985A1 - Corynebacterium glutamicum genese encoding proteins involved in carbon metabolism and energy production - Google Patents

Description

P001 Section 29 Regulation 3.2(2)

AUSTRALIA

Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT Application Number: Lodged: Invention Title: Corynebacterium glutamicum genese encoding proteins CHECK! The following statement is a full description of this invention, including the best method of performing it known to us: -1- F- COR YNEBACTERIUM GLUTAMICUM GENES ENCODING PROTEINS INVOLVED IN CARBON METABOLISM AND ENERGY PRODUCTION Related Applications This application claims priority to prior U.S. Provisional Patent Application 00 Serial No. 60/141031, filed June 25, 1999, U.S. Provisional Patent Application Serial No. 60/143208, filed July 9, 1999, and U.S. Provisional Patent Application Serial No.

S60/151572, filed August 31, 1999. This application also claim's priority to prior German Patent Application No. 19931412.8, filed July 8, 1999, German Patent Application No.

19931413.6, filed July 8, 1999, German Patent Application No. 19931419.5, filed July 8, 1999, German Patent Application No.. 19931420.9, filed July 8, 1999, German Patent Application No. 19931424.1, filed July 8, 1999, German Patent Application No.

19931428.4, filed July 8, 1999, German Patent Application No. 19931431.4, filed July 8, 1999, German Patent Application No. 19931433.0, filed July 8, 1999, German Patent Application No. 19931434.9, filed July 1999, German Patent Application No.

19931510.8, filed July 8, 1999, German Patent Application No. 19931562.0, filed July 8, 1999, German Patent Application No. 19931634.1, filed July 8, 1999, German Patent Application No. 19932180.9, filed July 9, 1999, German Patent Application No.

19932227.9, filed July 9, 1999, German Patent Application No. 19932230.9, filed Juily 9, 1999, German Patent Application No. 19932924.9, filed July 14, 1999, German Patent Application No. 19932973.7, filed July 14, 1999, German Patent Application No.

19933005.0, filed July 14, 1999, German Patent Application No. 19940765.7, filed August 27, 1999, German Patent Application No. 19942076.9, filed September 3, 1999, German Patent Application No. 19942079.3, filed September 3, 1999, German Patent Application No. 19942086.6, filed September 3, 1999, German Patent Application No.

19942087.4, filed September 3, 1999, German Patent Application No. 19942088.2, filed September 3, 1999, German Patent Application No. 19942095.5, filed September 3, 1999, German Patent Application No. 19942123.4, filed September 3, 1999, and German Patent Application No. 19942125.0, filed September 3, 1999. The entire contents of all of the aforementioned application are hereby expressly incorporated herein by this reference.

-2- N Background of the Invention QCertain products and by-products of naturally-occurring metabolic processes in cells have utility in a wide array of industries, including the food, feed, cosmetics, and C pharmaceutical industries. These molecules, collectively termed 'fine chemicals', include organic acids, both proteinogenic and non-proteinogenic amino acids, O nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, and enzymes. Their production is most Sconveniently performed through the large-scale culture of bacteria developed to produce Sand secrete large quantities of one or more desired molecules. One particularly useful 10 organism for this purpose is Corynebacterium glutamicum, a gram positive, nonpathogenic bacterium. Through strain selection, a number of mutant strains have been developed which produce an array of desirable compounds. However, selection of strains improved for the production of a particular molecule is a time-consuming and difficult process.

Summary of the Invention The invention provides novel bacterial nucleic acid molecules which have a variety of uses. These uses include the identification of microorganisms which can be used to produce fine chemicals, the modulation of fine chemical production in C glutamicum or related bacteria, the typing or identification of C. glutamicum or related bacteria, as reference points for mapping the C. glutamicum genome, and as markers for transformation. These novel nucleic acid molecules encode proteins, referred to herein as sugar metabolism and oxidative phosphorylation (SMP) proteins.

C. glutamicum is a gram positive, aerobic bacterium which is commonly used in industry for the large-scale production of a variety of fine chemicals, and also for the degradation of hydrocarbons (such as in petroleum spills) and for the oxidation of terpenoids. The SMP nucleic acid molecules of the invention, therefore, can be used to identify microorganisms which can be used to produce fine chemicals, by fermentation processes. Modulation of the expression of the SMP nucleic acids of the invention, or modification of the sequence of the SMP nucleic acid molecules of the invention, can be used to modulate the production of one or more fine chemicals from a -3- C1 microorganism to improve the yield or production of one or more fine chemicals Sfrom a Corynebacterium or Brevibacterium species).

The SMP nucleic acids of the invention may also be used to identify an organism CN as being Corynebacterium glutamicum or a close relative thereof, or to identify the presence of C. glutamicum or a relative thereof in a mixed population of tO microorganisms. The invention provides the nucleic acid sequences of a number of C 0 glutamicum genes; by probing the extracted genomic DNA of a culture of a unique or 1 mixed population of microorganisms under stringent conditions with a probe spanning a Sregion of a C. glutamicum gene which is unique to this organism, one can ascertain whether this organism is present. Although Corynebacterium glutamicum itself is nonpathogenic, it is related to species pathogenic in humans, such as Corynebacterium diphtheriae (the causative agent of diphtheria); the detection of such organisms is of significant clinical relevance.

The SMP nucleic acid molecules of the invention may also serve as reference points for mapping of the C. glutamicum genome, or of genomes of related organisms.

Similarly, these molecules, or variants or portions thereof, may serve as markers for genetically engineered Corynebacterium or Brevibacterium species.

The SMP proteins encoded by the novel nucleic acid molecules of the invention are capable of, for example, performing a function involved in the metabolism of carbon compounds such as sugars or in the generation of energy molecules by processes such as oxidative phosphorylation in Corynebacterium glutamicum. Given the availability of cloning vectors for use in Corynebacterium glutamicum, such as those disclosed in Sinskey et al., U.S. Patent No. 4,649,119, and techniques for genetic manipulation of C.

glutamicum and the related Brevibacterium species lactofermentum) (Yoshihama et al, J. Bacteriol. 162: 591-597 (1985); Katsumata et al, J Bacteriol. 159: 306-311 (1984); and Santamaria et al., J. Gen. Microbiol. 130: 2237-2246 (1984)), the nucleic acid molecules of the invention may be utilized in the genetic engineering of this organism to make it a better or more efficient producer of one or more fine chemicals.

This improved production or efficiency of production of a fine chemical may be due to a direct effect of manipulation of a gene of the invention, or it may be due to an indirect effect of such manipulation.

-4- (1N There are a number of mechanisms by which the alteration of an SMP protein of ,.the invention may directly affect the yield, production, and/or efficiency of production of a fine chemical from a C. glutamicum strain incorporating such an altered protein.

The degradation of high-energy carbon molecules such as sugars, and the conversion of compounds such as NADH and FADH 2 to compounds containing high energy phosphate oO bonds via oxidative phosphorylation results in a number of compounds which themselves may be desirable fine chemicals, such as pyruvate, ATP, NADH, and a number of intermediate sugar compounds. Further, the energy molecules (such as ATP) Iand the reducing equivalents (such as NADH or NADPH) produced by these metabolic 10 pathways are utilized in the cell to drive reactions which would otherwise be energetically unfavorable. Such unfavorable reactions include many biosynthetic pathways for fine chemicals. By improving the ability of the cell to utilize a particular sugar by manipulating the genes encoding enzymes involved in the degradation and conversion of that sugar into energy for the cell), one may increase the amount of energy available to permit unfavorable, yet desired metabolic reactions the biosynthesis of a desired fine chemical) to occur.

The mutagenesis of one or more SMP genes of the invention may also result in SMP proteins having altered activities which indirectly impact the production of one or more desired fine chemicals from C. glutamicurn. For example, by increasing the efficiency of utilization of one or more sugars (such that the conversion of the sugar to useful energy molecules is improved), or by increasing the efficiency of conversion of reducing equivalents to useful energy molecules by improving the efficiency of oxidative phosphorylation, or the activity of the ATP synthase), one can increase the amount of these high-energy compounds available to the cell to drive normally unfavorable metabolic processes. These processes include the construction of cell walls, transcription, translation, and the biosynthesis of compounds necessary for growth and division of the cells nucleotides, amino acids, vitamins, lipids, etc.) (Lengeler et al.

(1999) Biology of Prokaryotes, Thieme Verlag: Stuttgart, p. 88-109; 913-918; 875-899).

By improving the growth and multiplication of these engineered cells, it is possible to increase both the viability of the cells in large-scale culture, and also to improve their rate of division, such that a relatively larger number of cells can survive in fermentor culture. The yield, production, or efficiency of production may be increased, at least due to the presence of a greater number of viable cells, each producing the c- desired fine chemical. Also, many of the degradation products produced during sugar metabolism are utilized by the cell as precursors or intermediates in the production of other desirable products, such as fine chemicals. So, by increasing c 5 the ability of the cell to metabolize sugars, the number of these degradation products available to the cell for other processes should also be increased.

0 The invention provides novel nucleic acid molecules which encode 0proteins, referred to herein as SMP proteins, which are capable of, for example,

(N

IND performing a function involved in the metabolism of carbon compounds such as C 10 sugars and the generation of energy molecules by processes such as oxidative phosphorylation in Corynebacterium glutamicum. Nucleic acid molecules encoding an SMP protein are referred to herein as SMP nucleic acid molecules.

In a preferred embodiment, the SMP protein participates in the conversion of carbon molecules and degradation products thereof to energy which is utilized by the cell for metabolic processes. Examples of such proteins include those encoded by the genes set forth in Table 1.

The following embodiments, the subject of this application, are specifically disclosed herein: SAn isolated Corynebacterium glutamicum nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 781 provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

0 An isolated nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs; 4 to 782, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

6 An isolated nucleic acid molecule which encodes a naturally

O

c occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 782, provided that the nucleic acid N 5 molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

oo An isolated nucleic acid molecule comprising a nucleotide sequence Swhich is at least 50% identical to the entire nucleotide sequence of N any of the odd numbered SEQ ID NOs: 3 to 781, provided that the 10 nucleic acid molecule does not consist of any of the F-designated c genes set forth in Table 1, or a complement thereof.

*An isolated nucleic acid molecule comprising a fragment of at least contiguous nucleotides of the nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 781, provided that the nucleic acid molecule does not consist of any of the F -designated genes set forth in Table 1, or a complement thereof.

*A method of producing a polypeptide, the method comprising culturing the host cell of claim 9 in an appropriate culture medium to, thereby, produce the polypeptide.

An isolated polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 782, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1.

An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 782, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1.

An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least identical to the entire nucleic acid sequence of any of the odd numbered SEQ ID NOs: 3 to 781, provided that the nucleic acid O molecule does not consist of any of the F-designated nucleic acid molecules set forth in Table 1.

An isolated polypeptide comprising an amino acid sequence which is at least 50% identical to the entire amino acid sequence of any of 5 the even numbered SEQ ID NOs: 4 to 782, provided that the amino acid sequence is not encoded by any of the F-designated genes set oo forth in Table 1.

An isolated polypeptide comprising a fragment of a polypeptide N comprising the amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 782, provided that the amino acid sequence is Snot encoded by any of the F-designated genes set forth in Table 1, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino sequence.

An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 781, provided that the nucleic acid molecule does not consist of any of the F-designated nucleic acid molecules set forth in Table 1.

A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 781, wherein the nucleic acid molecule is disrupted.

A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 781, wherein the nucleic acid molecule comprises one or more nucleic acid modifications as compared to the sequence set forth in any of the odd-numbered SEQ ID NOs: 3 to 781.

A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 781, wherein the regulatory region of the nucleic acid molecule is modified relative to the wild-type regulatory region of the molecule.

An isolated Corynebacterium glutamicum nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO: 179, or a complement thereof.

An isolated nucleic acid molecule which encodes a polypeptide c( comprising the amino acid sequence set forth in SEQ ID NO:180, or a complement thereof.

An isolated nucleic acid molecule which encodes a naturally 5 occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID No: 180, or a complement thereof.

oo An isolated nucleic acid molecule comprising a nucleotide sequence O which is at least 50% identical to the entire nucleotide sequence set forth in SEQ ID No: 179, or a complement thereof.

An isolated nucleic acid molecule comprising a fragment of at least contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO: 179, or a complement thereof.

An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:180.

An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 180.

An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least identical to the entire nucleotide sequence set forth in SEQ ID NO:179.

An isolated polypeptide comprising an amino acid sequence which is at least 50% identical to the entire amino acid sequence set forth in SEQ ID NO:180.

An isolated polypeptide comprising a fragment of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:180, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino sequence set forth in SEQ ID NO:180.

An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence as set forth in SEQ ID NO: 179.

0 A host cell comprising the nucleic acid molecule set forth in SEQ ID

O

cN NO: 179, wherein the nucleic acid molecule is disrupted.

A host cell comprising the nucleic acid molecule set forth in SEQ ID NO: 179, wherein the nucleic acid molecule comprises one or more l 5 nucleic acid modifications as compared to the sequence set forth in SEQ ID NO:179.

oo A host cell comprising the nucleic acid molecule set forth in SEQ ID SNO:179, wherein the regulatory region of the nucleic acid molecule Sis modified relative to the wild-type regulatory region of the 10 molecule.

Accordingly, one aspect of the invention pertains to isolated nucleic acid molecules cDNAs, DNAs, or RNAs) comprising a nucleotide sequence encoding an SMP protein or biologically active portions thereof, as well as nucleic acid fragments suitable as primers or hybridization probes for the detection or amplification of SMP-encoding nucleic acid DNA or mRNA). In particularly preferred embodiments, the isolated nucleic acid molecule comprises one of the nucleotide sequences set forth as the odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID or the coding region or a complement thereof of one of these nucleotide sequences. In other particularly preferred embodiments, the isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes to or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80% or 90%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence set forth as an odd-numbered SEQ ID NO in the Sequence Listing SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID or a portion thereof. In other preferred embodiments, the isolated nucleic acid molecule encodes one of the amino acid sequences set forth as an even-numbered SEQ ID NO in the Sequence Listing SEQ ID NO:2, SEQ ID NO:4, SEQ (N ID NO:6, SEQ ID The preferred SMP proteins of the present invention also Spreferably possess at least one of the SMP activities described herein.

In another embodiment, the isolated nucleic acid molecule encodes a protein or portion thereof wherein the protein or portion thereof includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a 00 sequence having an even-numbered SEQ ID NO: in the Sequence Listing), e.g., a sufficiently homologous to an amino acid sequence of the invention such that the protein C or portion thereof maintains an SMP activity. Preferably, the protein or portion thereof I encoded by the nucleic acid molecule maintains the ability to perform a function 10 involved in the metabolism of carbon compounds such as sugars or the generation of energy molecules ATP) by processes such as oxidative phosphorylation in Corynebacterium glutamicum. In one embodiment, the protein encoded by the nucleic acid molecule is at least about 50%, preferably at least about 60%, and more preferably at least about 70%, 80%, or 90% and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an amino acid sequence of the invention an entire amino acid sequence selected those having an even-numbered SEQ ID NO in the Sequence Listing). In another preferred embodiment, the protein is a full length C.

glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention (encoded by an open reading frame shown in the corresponding oddnumbered SEQ ID NOs in the Sequence Listing SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID In another preferred embodiment, the isolated nucleic acid molecule is derived from C. glutamicum and encodes a protein an SMP fusion protein) which includes a biologically active domain which is at least about 50% or more homologous to one of the amino acid sequences of the invention a sequence of one of the even-numbered SEQ ID NOs in the Sequence Listing) and is able to perform a function involved in the metabolism of carbon compounds such as sugars or the generation of energy molecules ATP) by processes such as oxidative phosphorylation in Corynebacterium glutamicum, or has one or more of the activities set forth in Table 1, and which also includes heterologous nucleic acid sequences encoding a heterologous polypeptide or regulatory regions.

(1N In another embodiment, the isolated nucleic acid molecule is at least ,.nucleotides in length and hybridizes under stringent conditions to a nucleic acid molecule comprising a nucleotide sequence of the invention a sequence of an odd- C1 numbered SEQ ID NO in the Sequence Listing) A. Preferably, the isolated nucleic acid molecule corresponds to a naturally-occurring nucleic acid molecule. More preferably, 00 the isolated nucleic acid encodes a naturally-occurring C. glutamicum SMP protein, or a biologically active portion thereof.

Another aspect of the invention pertains to vectors, recombinant expression Ivectors, containing the nucleic acid molecules of the invention, and host cells into which 10 such vectors have been introduced. In one embodiment, such a host cell is used to produce an SMP protein by culturing the host cell in a suitable medium. The SMP protein can be then isolated from the medium or the host cell.

Yet another aspect of the invention pertains to a genetically altered microorganism in which an SMP gene has been introduced or altered. In one embodiment, the genome of the microorganism has been altered by introduction of a nucleic acid molecule of the invention encoding wild-type or mutated SMP sequence as a transgene. In another embodiment, an endogenous SMP gene within the genome of the microorganism has been altered, functionally disrupted, by homologous recombination with an altered SMP gene. In another embodiment, an endogenous or introduced SMP gene in a microorganism has been altered by one or more point mutations, deletions, or inversions, but still encodes a functional SMP protein. In still another embodiment, one or more of the regulatory regions a promoter, repressor, or inducer) of an SMP gene in a microorganism has been altered by deletion, truncation, inversion, or point mutation) such that the expression of the SMP gene is modulated. In a preferred embodiment, the microorganism belongs to the genus Corynebacterium or Brevibacterium, with Corynebacterium glutamicum being particularly preferred. In a preferred embodiment, the microorganism is also utilized for the production of a desired compound, such as an amino acid, with lysine being particularly preferred.

In another aspect, the invention provides a method of identifying the presence or activity of Cornyebacterium diphtheriae in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention the -8- (N sequences set forth in the Sequence Listing as SEQ ID NOs 1 through 782) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject.

Still another aspect of the invention pertains to an isolated SMP protein or a portion, a biologically active portion, thereof. In a preferred embodiment, the isolated SMP protein or portion thereof is capable of performing a function involved in 00 the metabolism of carbon compounds such as sugars or in the generation of energy 0 molecules ATP) by processes such as oxidative phosphorylation in C Corynebacterium glutamicum. In another preferred embodiment, the isolated SMP Sprotein or portion thereof is sufficiently homologous to an amino acid sequence of the 10 invention a sequence of an even-numbered SEQ ID NO: in the Sequence Listing) such that the protein or portion thereof maintains the ability to perform a function involved in the metabolism of.carbon compounds such as sugars or in the generation of energy molecules ATP) by processes such as oxidative phosphorylation in Corynebacterium glutamicum.

The invention also provides an isolated preparation of an SMP protein. In preferred embodiments, the SMP protein comprises an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing).

In another preferred embodiment, the invention pertains to an isolated full length protein which is substantially homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) (encoded by an open reading frame set forth in a corresponding odd-numbered SEQ ID NO: of the Sequence Listing). In yet another embodiment, the protein is at least about preferably at least about 60%, and more preferably at least about 70%, 80%, or and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing). In other embodiments, the isolated SMP protein comprises an amino acid sequence which is at least about 50% or more homologous to one of the amino acid sequences of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) and is able to perform a function involved in the metabolism of carbon compounds such as sugars or in the generation of energy molecules ATP) by processes such as oxidative phosphorylation in Corynebacterium glutamicum, or has one or more of the activities set forth in Table 1.

-9- I Alternatively, the isolated SMP protein can comprise an amino acid sequence Swhich is encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, or is at least about 50%, preferably at least about 60%, more N preferably at least about 70%, 80%, or 90%, and even more preferably at least about 95%, 96%, 97%, or 99% or more homologous to a nucleotide sequence of one of Sthe even-numbered SEQ ID NOs set forth in the Sequence Listing. It is also preferred 00 that the preferred forms of SMP proteins also have one or more of the SMP bioactivities Sdescribed herein.

IDThe SMP polypeptide, or a biologically active portion thereof, can be operatively linked to a non-SMP polypeptide to form a fusion protein. In preferred embodiments, this fusion protein has an activity which differs from that of the SMP protein alone. In other preferred embodiments, this fusion protein performs a function involved in the metabolism of carbon compounds such as sugars or in the generation of energy molecules ATP) by processes such as oxidative phosphorylation in Corynebacterium glutamicum. In particularly preferred embodiments, integration of this fusion protein into a host cell modulates production of a desired compound from the cell.

In another aspect, the invention provides methods for screening molecules which modulate the activity of an SMP protein, either by interacting with the protein itself or a substrate or binding partner of the SMP protein, or by modulating the transcription or translation of an SMP nucleic acid molecule of the invention.

Another aspect of the invention pertains to a method for producing a fine chemical. This method involves the culturing of a cell containing a vector directing the expression of an SMP nucleic acid molecule of the invention, such that a fine chemical is produced. In a preferred embodiment, this method further includes the step of obtaining a cell containing such a vector, in which a cell is transfected with a vector directing the expression of an SMP nucleic acid. In another preferred embodiment, this method further includes the step of recovering the fine chemical from the culture. In a particularly preferred embodiment, the cell is from the genus Corynebacterium or Brevibacterium, or is selected from those strains set forth in Table 3.

Another aspect of the invention pertains to methods for modulating production of a molecule from a microorganism. Such methods include contacting the cell with an agent which modulates SMP protein activity or SMP nucleic acid expression such that a cell associated activity is altered relative to this same activity in the absence of the Sagent. In a preferred embodiment, the cell is modulated for one or more C. glutamicum carbon metabolism pathways or for the production of energy through processes such as oxidative phosphorylation, such that the yields or rate of production of a desired fine chemical by this microorganism is improved. The agent which modulates SMP protein 00 activity can be an agent which stimulates SMP protein activity or SMP nucleic acid Sexpression. Examples of agents which stimulate SMP protein activity or SMP nucleic N acid expression include small molecules, active SMP proteins, and nucleic acids encoding SMP proteins that have been introduced into the cell. Examples of agents which inhibit SMP activity or expression include small molecules and antisense SMP nucleic acid molecules.

Another aspect of the invention pertains to methods for modulating yields of a desired compound from a cell, involving the introduction of a wild-type or mutant SMP gene into a cell, either maintained on a separate plasmid or integrated into the genome of the host cell. If integrated into the genome, such integration can be random, or it can take place by homologous recombination such that the native gene is replaced by the introduced copy, causing the production of the desired compound from the cell to be modulated. In a preferred embodiment, said yields are increased. In another preferred embodiment, said chemical is a fine chemical. In a particularly preferred embodiment, said fine chemical is an amino acid. In especially preferred embodiments, said amino acid is L-lysine.

Detailed Description of the Invention The present invention provides SMP nucleic acid and protein molecules which are involved in the metabolism of carbon compounds such as sugars and the generation of energy molecules by processes such as oxidative phosphorylation in Corynebacterium glutamicum. The molecules of the invention may be utilized in the modulation of production of fine chemicals from microorganisms, such as C.

glutamicum, either directly where overexpression or optimization of a glycolytic pathway protein has a direct impact on the yield, production, and/or efficiency of production of, pyruvate from modified C. glutamicum), or may have an indirect 11 (N1 impact which nonetheless results in an increase of yield, production, and/or efficiency of Sproduction of the desired compound where modulation of proteins involved in oxidative phosphorylation results in alterations in the amount of energy available to C perform necessary metabolic processes and other cellular functions, such as nucleic acid and protein biosynthesis and transcription/translation). Aspects of the invention are 0 further explicated below.

I. Fine Chemicals The term 'fine chemical' is art-recognized and includes molecules produced by an organism which have applications in various industries, such as, but not limited to, the pharmaceutical, agriculture, and cosmetics industries. Such compounds include organic acids, such as tartaric acid, itaconic acid, and diaminopimelic acid, both proteinogenic and non-proteinogenic amino acids, purine and pyrimidine bases, nucleosides, and nucleotides (as described e.g. in Kuninaka, A. (1996) Nucleotides and related compounds, p. 561-612, in Biotechnology vol. 6, Rehm el al., eds. VCH: Weinheim, and references contained therein), lipids, both saturated and unsaturated fatty acids arachidonic acid), diols propane diol, and butane diol), carbohydrates hyaluronic acid and trehalose), aromatic compounds aromatic amines, vanillin, and indigo), vitamins and cofactors (as described in Ullmann's Encyclopedia of Industrial Chemistry, vol. A27, "Vitamins", p. 443-613 (1996) VCH: Weinheim and references therein; and Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press, (1995)), enzymes, polyketides (Cane et al. (1998) Science 282: 63-68), and all other chemicals described in Gutcho (1983) Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086 and references therein. The metabolism and uses of certain of these fine chemicals are further explicated below.

A. Amino Acid Metabolism and Uses Amino acids comprise the basic structural units of all proteins, and as such are essential for normal cellular functioning in all organisms. The term "amino acid" is art- -12- CN recognized. The proteinogenic amino acids, of which there are 20 species, serve as Sstructural units for proteins, in which they are linked by peptide bonds, while the nonproteinogenic amino acids (hundreds of which are known) are not normally found in Cproteins (see Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97 VCH: Weinheim (1985)). Amino acids may be in the D- or L- optical configuration, though L- 00 amino acids are generally the only type found in naturally-occurring proteins.

Biosynthetic and degradative pathways of each of the 20 proteinogenic amino acids 0 have been well characterized in both prokaryotic and eukaryotic cells (see, for example, I Stryer, L. Biochemistry, 3 rd edition, pages 578-590 (1988)). The 'essential' amino acids (histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine), so named because they are generally a nutritional requirement due to the complexity of their biosyntheses, are readily converted by simple biosynthetic pathways to the remaining 11 'nonessential' amino acids (alanine, arginine, asparagine, aspartate, cysteine, glutamate, glutamine, glycine, proline, serine, and tyrosine). Higher animals do retain the ability to synthesize some of these amino acids, but the essential amino acids must be supplied from the diet in order for normal protein synthesis to occur.

Aside from their function in protein biosynthesis, these amino acids are interesting chemicals in their own right, and many have been found to have various applications in the food, feed, chemical, cosmetics, agriculture, and pharmaceutical industries. Lysine is an important amino acid in the nutrition not only of humans, but also of monogastric animals such as poultry and swine. Glutamate is most commonly used as a flavor additive (mono-sodium glutamate, MSG) and is widely used throughout the food industry, as are aspartate, phenylalanine, glycine, and cysteine. Glycine, Lmethionine and tryptophan are all utilized in the pharmaceutical industry. Glutamine, valine, leucine, isoleucine, histidine, arginine, proline, serine and alanine are of use in both the pharmaceutical and cosmetics industries. Threonine, tryptophan, and D/ Lmethionine are common feed additives. (Leuchtenberger, W. (1996) Amino aids technical production and use, p. 466-502 in Rehm et al. (eds.) Biotechnology vol. 6, chapter 14a, VCH: Weinheim). Additionally, these amino acids have been found to be useful as precursors for the synthesis of synthetic amino acids and proteins, such as Nacetylcysteine, S-carboxymethyl-L-cysteine, (S)-5-hydroxytryptophan, and others -13 CN described in Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97, VCH: SWeinheim, 1985.

The biosynthesis of these natural amino acids in organisms capable of CI producing them, such as bacteria, has been well characterized (for review of bacterial amino acid biosynthesis and regulation thereof, see Umbarger, H.E.(1978) Ann. Rev.

k Biochem. 47: 533-606). Glutamate is synthesized by the reductive amination ofa- O ketoglutarate, an intermediate in the citric acid cycle. Glutamine, proline, and arginine are each subsequently produced from glutamate. The biosynthesis of serine is a three- Sstep process beginning with 3-phosphoglycerate (an intermediate in glycolysis), and 10 resulting in this amino acid after oxidation, transamination, and hydrolysis steps. Both cysteine and glycine are produced from serine; the former by the condensation of homocysteine with serine, and the latter by the transferal of the side-chain p-carbon atom to tetrahydrofolate, in a reaction catalyzed by serine transhydroxymethylase.

Phenylalanine, and tyrosine are synthesized from the glycolytic and pentose phosphate pathway precursors erythrose 4-phosphate and phosphoenolpyruvate in a 9-step biosynthetic pathway that differ only at the final two steps after synthesis of prephenate.

Tryptophan is also produced from these two initial molecules, but its synthesis is an 11step pathway. Tyrosine may also be synthesized from phenylalanine, in a reaction catalyzed by phenylalanine hydroxylase. Alanine, valine, and leucine are all biosynthetic products of pyruvate, the final product of glycolysis. Aspartate is formed from oxaloacetate, an intermediate of the citric acid cycle. Asparagine, methionine, threonine, and lysine are each produced by the conversion ofaspartate. Isoleucine is formed from threonine. A complex 9-step pathway results in the production of histidine from 5-phosphoribosyl-1-pyrophosphate, an activated sugar.

Amino acids in excess of the protein synthesis needs of the cell cannot be stored, and are instead degraded to provide intermediates for the major metabolic pathways of the cell (for review see Stryer, L. Biochemistry 3 rd ed. Ch. 21 "Amino Acid Degradation and the Urea Cycle" p. 495-516 (1988)). Although the cell is able to convert unwanted amino acids into useful metabolic intermediates, amino acid production is costly in terms of energy, precursor molecules, and the enzymes necessary to synthesize them.

Thus it is not surprising that amino acid biosynthesis is regulated by feedback inhibition, in which the presence of a particular amino acid serves to slow or entirely stop its own -14production (for overview of feedback mechanisms in amino acid biosynthetic pathways, Ssee Stryer, L. Biochemistry, 3 rd ed. Ch. 24: "Biosynthesis of Amino Acids and Heme" p.

575-600 (1988)). Thus, the output of any particular amino acid is limited by the amount of that amino acid present in the cell.

00 B. Vitamin, Cofactor, and Nutraceutical Metabolism and Uses O Vitamins, cofactors, and nutraceuticals comprise another group of molecules C which the higher animals have lost the ability to synthesize and so must ingest, although Sthey are readily synthesized by other organisms such as bacteria. These molecules are C1 10 either bioactive substances themselves, or are precursors of biologically active substances which may serve as electron carriers or intermediates in a variety of metabolic pathways. Aside from their nutritive value, these compounds also have significant industrial value as coloring agents, antioxidants, and catalysts or other processing aids. (For an overview of the structure, activity, and industrial applications of these compounds, see, for example, Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996.) The term "vitamin" is artrecognized, and includes nutrients which are required by an organism for normal functioning, but which that organism cannot synthesize by itself. The group of vitamins may encompass cofactors and nutraceutical compounds. The language "cofactor" includes nonproteinaceous compounds required for a normal enzymatic activity to occur. Such compounds may be organic or inorganic; the cofactor molecules of the invention are preferably organic. The term "nutraceutical" includes dietary supplements having health benefits in plants and animals, particularly humans. Examples of such molecules are vitamins, antioxidants, and also certain lipids polyunsaturated fatty acids).

The biosynthesis of these molecules in organisms capable of producing them, such as bacteria, has been largely characterized (Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley Sons; Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological N Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept.

S1-3, 1994 at Penang, Malaysia, AOCS Press: Champaign, IL X, 374 S).

Thiamin (vitamin B 1 is produced by the chemical coupling of pyrimidine and C thiazole moieties. Riboflavin (vitamin B 2 is synthesized from (GTP) and ribose-5'-phosphate. Riboflavin, in turn, is utilized for the synthesis of flavin OC mononucleotide (FMN) and flavin adenine dinucleotide (FAD). The family of compounds collectively termed 'vitamin B 6 pyridoxine, pyridoxamine, pyridoxaand the commercially used pyridoxin hydrochloride) are all derivatives of Sthe common structural unit, 5-hydroxy-6-methylpyridine. Pantothenate (pantothenic acid, (R)-(+)-N-(2,4-dihydroxy-3,3-dimethyl-l -oxobutyl)-P-alanine) can be produced either by chemical synthesis or by fermentation. The final steps in pantothenate biosynthesis consist of the ATP-driven condensation of p-alanine and pantoic acid. The enzymes responsible for the biosynthesis steps for the conversion to pantoic acid, to palanine and for the condensation to panthotenic acid are known. The metabolically active form of pantothenate is Coenzyme A, for which the biosynthesis proceeds in enzymatic steps. Pantothenate, pyridoxal-5'-phosphate, cysteine and ATP are the precursors of Coenzyme A. These enzymes not only catalyze the formation of panthothante, but also the production of (R)-pantoic acid, (R)-pantolacton, panthenol (provitamin B 5 pantetheine (and its derivatives) and coenzyme A.

Biotin biosynthesis from the precursor molecule pimeloyl-CoA in microorganisms has been studied in detail and several of the genes involved have been identified. Many of the corresponding proteins have been found to also be involved in Fe-cluster synthesis and are members of the nifS class of proteins. Lipoic acid is derived from octanoic acid, and serves as a coenzyme in energy metabolism, where it becomes part of the pyruvate dehydrogenase complex and the a-ketoglutarate dehydrogenase complex. The folates are a group of substances which are all derivatives of folic acid, which is turn is derived from L-glutamic acid, p-amino-benzoic acid and 6methylpterin. The biosynthesis of folic acid and its derivatives, starting from the metabolism intermediates guanosine-5'-triphosphate (GTP), L-glutamic acid and pamino-benzoic acid has been studied in detail in certain microorganisms.

Corrinoids (such as the cobalamines and particularly vitamin B 12 and porphyrines belong to a group of chemicals characterized by a tetrapyrole ring system.

-16- CN The biosynthesis of vitamin B 1 2 is sufficiently complex that it has not yet been d completely characterized, but many of the enzymes and substrates involved are now known. Nicotinic acid (nicotinate), and nicotinamide are pyridine derivatives which are C1 also termed 'niacin'. Niacin is the precursor of the important coenzymes NAD (nicotinamide adenine dinucleotide) and NADP (nicotinamide adenine dinucleotide 0o phosphate) and their reduced forms.

The large-scale production of these compounds has largely relied on cell-free chemical syntheses, though some of these chemicals have also been produced by large-

ID

scale culture of microorganisms, such as riboflavin, Vitamin B 6 pantothenate, and 10 biotin. Only Vitamin B12 is produced solely by fermentation, due to the complexity of its synthesis. In vitro methodologies require significant inputs of materials and time, often at great cost.

C. Purine, Pyrimidine, Nucleoside and Nucleotide Metabolism and Uses Purine and pyrimidine metabolism genes and their corresponding proteins are important targets for the therapy of tumor diseases and viral infections. The language "purine" or "pyrimidine" includes the nitrogenous bases which are constituents of nucleic acids, co-enzymes, and nucleotides. The term "nucleotide" includes the basic structural units of nucleic acid molecules, which are comprised of a nitrogenous base, a pentose sugar (in the case of RNA, the sugar is ribose; in the case of DNA, the sugar is D-deoxyribose), and phosphoric acid. The language "nucleoside" includes molecules which serve as precursors to nucleotides, but which are lacking the phosphoric acid moiety that nucleotides possess. By inhibiting the biosynthesis of these molecules, or their mobilization to form nucleic acid molecules, it is possible to inhibit RNA and DNA synthesis; by inhibiting this activity in a fashion targeted to cancerous cells, the ability of tumor cells to divide and replicate may be inhibited. Additionally, there are nucleotides which do not form nucleic acid molecules, but rather serve as energy stores AMP) or as coenzymes FAD and NAD).

Several publications have described the use of these chemicals for these medical indications, by influencing purine and/or pyrimidine metabolism Christopherson, R.I. and Lyons, S.D. (1990) "Potent inhibitors of de novo pyrimidine and purine biosynthesis as chemotherapeutic agents." Med Res. Reviews 10: 505-548). Studies of 17enzymes involved in purine and pyrimidine metabolism have been focused on the development of new drugs which can be used, for example, as immunosuppressants or Santi-proliferants (Smith, (1995) "Enzymes in nucleotide synthesis." Curr. Opin.

Struct. Biol. 5: 752-757; (1995) Biochem Soc. Transact. 23: 877-902). However, purine and pyrimidine bases, nucleosides and nucleotides have other utilities: as intermediates in the biosynthesis of several fine chemicals thiamine, S-adenosyl-methionine, folates, or riboflavin), as energy carriers for the cell ATP or GTP), and for chemicals themselves, commonly used as flavor enhancers IMP or GMP) or for Sseveral medicinal applications (see, for example, Kuninaka, A. (1996) Nucleotides and Related Compounds in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, p. 561- 612). Also, enzymes involved in purine, pyrimidine, nucleoside, or nucleotide metabolism are increasingly serving as targets against which chemicals for crop protection, including fungicides, herbicides and insecticides, are developed.

The metabolism of these compounds in bacteria has been characterized (for reviews see, for example, Zalkin, H. and Dixon, J.E. (1992) "de novo purine nucleotide biosynthesis", in: Progress in Nucleic Acid Research and Molecular Biology, vol. 42, Academic Press:, p. 259-287; and Michal, G. (1999) "Nucleotides and Nucleosides", Chapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York). Purine metabolism has been the subject of intensive research, and is essential to the normal functioning of the cell. Impaired purine metabolism in higher animals can cause severe disease, such as gout. Purine nucleotides are synthesized from in a series of steps through the intermediate compound phosphate (IMP), resulting in the production of guanosine-5'-monophosphate (GMP) or (AMP), from which the triphosphate forms utilized as nucleotides are readily formed. These compounds are also utilized as energy stores, so their degradation provides energy for many different biochemical processes in the cell.

Pyrimidine biosynthesis proceeds by the formation of uridine-5'-monophosphate

(UMP)

from ribose-5-phosphate. UMP, in turn, is converted to cytidine-5'-triphosphate

(CTP).

The deoxy- forms of all of these nucleotides are produced in a one step reduction reaction from the diphosphate ribose form of the nucleotide to the diphosphate deoxyribose form of the nucleotide. Upon phosphorylation, these molecules are able to participate in DNA synthesis.

18- D. Trehalose Metabolism and Uses Trehalose consists of two glucose molecules, bound in a, a-1,l linkage. It is C commonly used in the food industry as a sweetener, an additive for dried or frozen foods, and in beverages. However, it also has applications in the pharmaceutical, to cosmetics and biotechnology industries (see, for example, Nishimoto et al., (1998) U.S.

O Patent No. 5,759,610; Singer, M.A. and Lindquist, S. (1998) Trends Biotech. 16: 460- S467; Paiva, C.L.A. and Panek, A.D. (1996) Biotech. Ann. Rev. 2: 293-314; and N Shiosaka, M. (1997) J. Japan 172: 97-102). Trehalose is produced by enzymes from many microorganisms and is naturally released into the surrounding medium, from which it can be collected using methods known in the art.

II. Sugar and Carbon Molecule Utilization and Oxidative Phosphorylation Carbon is a critically important element for the formation of all organic compounds, and thus is a nutritional requirement not only for the growth and division of C. glutamicum, but also for the overproduction of fine chemicals from this microorganism. Sugars, such as mono-, di-, or polysaccharides, are particularly good carbon sources, and thus standard growth media typically contain one or more of: glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch, or cellulose (Ullmann's Encyclopedia of Industrial Chemistry (1987) vol. A9, "Enzymes", VCH: Weinheim). Alternatively, more complex forms of sugar may be utilized in the media, such as molasses, or other by-products of sugar refinement. Other compounds aside from the sugars may be used as alternate carbon sources, including alcohols ethanol or methanol), alkanes, sugar alcohols, fatty acids, and organic acids acetic acid or lactic acid). For a review of carbon sources and their utilization by microorganisms in culture, see: Ullman's Encyclopedia of Industrial Chemistry (1987) vol. A9, "Enzymes", VCH: Weinheim; Stoppok, E. and Buchholz, K. (1996) "Sugar-based raw materials for fermentation applications" in Biotechnology (Rehm, H.J. et al., eds.) vol. 6, VCH: Weinheim, p. 5-29; Rehm, H.J.

(1980) Industrielle Mikrobiologie, Springer: Berlin; Bartholomew, and Reiman, H.B. (1979). Economics of Fermentation Processes, in: Peppier, H.J. and Perlman, D., eds. Microbial Technology 2 nd ed., vol. 2, chapter 18, Academic Press: New York; and -19- (1N Kockova-Kratachvilova, A. (1981) Characteristics of Industrial Microorganisms, in: dRehm, H.J. and Reed, eds. Handbook of Biotechnology, vol. 1, chapter 1, Verlag Chemie: Weinheim.

c1 After uptake, these energy-rich carbon molecules must be processed such that they are able to be degraded by one of the major sugar metabolic pathways. Such 00 pathways lead directly to useful degradation products, such as ribose-5-phosphate and phosphoenolpyruvate, which may be subsequently converted to pyruvate. Three of the most important pathways in bacteria for sugar metabolism include the Embden- SMeyerhoff-Pamas (EMP) pathway (also known as the glycolytic or fructose 10 bisphosphate pathway), the hexosemonophosphate (HMP) pathway (also known as the pentose shunt or pentose phosphate pathway), and the Entner-Doudoroff (ED) pathway (for review, see Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York, and Stryer, L. (1988) Biochemistry, Chapters 13-19, Freeman: New York, and references therein).

The EMP pathway converts hexose molecules to pyruvate, and in the process produces 2 molecules of ATP and 2 molecules of NADH. Starting with glucose-1phosphate (which may be either directly taken up from the medium, or alternatively may be generated from glycogen, starch, or cellulose), the glucose molecule is isomerized to fructose-6-phosphate, is phosphorylated, and split into two 3-carbon molecules of glyceraldehyde-3-phosphate. After dehydrogenation, phosphorylation, and successive rearrangements, pyruvate results.

The HMP pathway converts glucose to reducing equivalents, such as NADPH, and produces pentose and tetrose compounds which are necessary as intermediates and precursors in a number of other metabolic pathways. In the HMP pathway, glucose-6phosphate is converted to ribulose-5-phosphate by two successive dehydrogenase reactions (which also release two NADPH molecules), and a carboxylation step.

may also be converted to xyulose-5-phosphate and phosphate; the former can undergo a series of biochemical steps to glucose-6-phosphate, which may enter the EMP pathway, while the latter is commonly utilized as an intermediate in other biosynthetic pathways within the cell.

The ED pathway begins with the compound glucose or gluconate, which is subsequently phosphorylated and dehydrated to form 2-dehydro-3-deoxy-6-P-gluconate.

(N1 Glucuronate and galacturonate may also be converted to 2-dehydro-3-deoxy-6-P- Sgluconate through more complex biochemical pathways. This product molecule is subsequently cleaved into glyceraldehyde-3-P and pyruvate; glyceraldehyde-3-P may C itself also be converted to pyruvate.

The EMP and HMP pathways share many features, including intermediates and 00 enzymes. The EMP pathway provides the greatest amount of ATP, but it does not produce ribose-5-phosphate, an important precursor for, nucleic acid biosynthesis, nor does it produce erythrose-4-phosphate, which is important for amino acid Sbiosynthesis. Microorganisms that are capable of using only the EMP pathway for glucose utilization are thus not able to grow on simple media with glucose as the sole carbon source. They are referred to as fastidious organisms, and their growth requires inputs of complex organic compounds, such as those found in yeast extract.

In contrast, the HMP pathway produces all of the precursors necessary for both nucleic acid and amino acid biosynthesis, yet yields only half the amount of ATP energy that the EMP pathway does. The HMP pathway also produces NADPH, which may be used for redox reactions in biosynthetic pathways. The HMP pathway does not directly produce pyruvate, however, and thus these microorganisms must also possess this portion of the EMP pathway. It is therefore not surprising that a number of microorganisms, particularly the facultative anerobes, have evolved such that they possess both of these pathways.

The ED pathway has thus far has only been found in bacteria. Although this pathway is linked partly to the HMP pathway in the reverse direction for precursor formation, the ED pathway directly forms pyruvate by the aldolase cleavage of 3ketodeoxy-6-phosphogluconate. The ED pathway can exist on its own and is utilized by the majority of strictly aerobic microorganisms. The net result is similar to that of the HMP pathway, although one mole of ATP can be formed only if the carbon atoms are converted into pyruvate, instead of into precursor molecules.

The pyruvate molecules produced through any of these pathways can be readily converted into energy via the Krebs cycle (also known as the citric acid cycle, the citrate cycle, or the tricarboxylic acid cycle (TCA cycle)). In this process, pyruvate is first decarboxylated, resulting in the production of one molecule ofNADH, 1 molecule of acetyl-CoA, and 1 molecule of CO 2 The acetyl group of acetyl CoA then reacts with -21 CN the 4 carbon unit, oxaolacetate, leading to the formation of citric acid, a 6 carbon organic acid. Dehydration and two additional CO 2 molecules are released. Ultimately, oxaloacetate is regenerated and can serve again as an acetyl acceptor, thus completing Ci the cycle. The electrons released during the oxidation of intermediates in the TCA cycle are transferred to NAD' to yield NADH.

OC During respiration, the electrons from NADH are transferred to molecular oxygen or other terminal electron acceptors. This process is catalyzed by the respiratory chain, an electron transport system containing both integral membrane proteins and Smembrane associated proteins. This system serves two basic functions: first, to accept 1 10 electrons from an electron donor and to transfer them to an electron acceptor, and second, to conserve some of the energy released during electron transfer by the synthesis of ATP. Several types of oxidation-reduction enzymes and electron transport proteins are known to be involved in such processes, including the NADH dehydrogenases, flavin-containing electron carriers, iron sulfur proteins, and cytochromes. The NADH dehydrogenases are located at the cytoplasmic surface of the plasma membrane, and transfer hydrogen atoms from NADH to flavoproteins, in turn accepting electrons from NADH. The flavoproteins are a group of electron carriers possessing a flavin prosthetic group which is alternately reduced and oxidized as it accepts and transfers electrons.

Three flavins are known to participate in these reactions: riboflavin, flavin-adenine dinucleotide (FAD) and flavin-mononucleotide (FMN). Iron sulfur proteins contain a cluster of iron and sulfur atoms which are not bonded to a heme group, but which still are able to participate in dehydration and rehydration reactions. Succinate dehydrogenase and aconitase are exemplary iron-sulfur proteins; their iron-sulfur complexes serve to accept and transfer electrons as part of the overall electron-transport chain. The cytochromes are proteins containing an iron porphyrin ring (heme). There are a number of different classes of cytochromes, differing in their reduction potentials.

Functionally, these cytochromes form pathways in which electrons may be transferred to other cytochromes having increasingly more positive reduction potentials. A further class of non-protein electron carriers is known: the lipid-soluble quinones coenzyme These molecules also serve as hydrogen atom acceptors and electron donors.

-22r- The action of the respiratory chain generates a proton gradient across the cell dmembrane, resulting in proton motive force. This force is utilized by the cell to synthesize ATP, via the membrane-spanning enzyme, ATP synthase. This enzyme is a

C

I multiprotein complex in which the transport of H molecules through the membrane results in the physical rotation of the intracellular subunits and concomitant 00 phosphorylation of ADP to form ATP (for review, see Fillingame, R.H. and Divall, S.

O (1999) Novartis Found Symp. 221: 218-229, 229-234).

C Non-hexose carbon substrates may also serve as carbon and energy sources for Scells. Such substrates may first be converted to hexose sugars in the gluconeogenesis C 10 pathway, where glucose is first synthesized by the cell and then is degraded to produce energy. The starting material for this reaction is phosphoenolpyruvate (PEP), which is one of the key intermediates in the glycolytic pathway. PEP may be formed from substrates other than sugars, such as acetic acid, or by decarboxylation of oxaloacetate (itself an intermediate in the TCA cycle). By reversing the glycolytic pathway (utilizing a cascade of enzymes different than those of the original glycolysis pathway), glucose-6phosphate may be formed. The conversion ofpyruvate to glucose requires the utilization of 6 high energy phosphate bonds, whereas glycolysis only produces 2 ATP in the conversion of glucose to pyruvate. However, the complete oxidation of glucose (glycolysis, conversion ofpyruvate into acetyl CoA, citric acid cycle, and oxidative phosphorylation) yields between 36-38 ATP, so the net loss of high energy phosphate bonds experienced during gluconeogenesis is offset by the overall greater gain in such high-energy molecules produced by the oxidation of glucose.

III. Elements and Methods of the Invention The present invention is based, at least in part, on the discovery of novel molecules, referred to herein as SMP nucleic acid and protein molecules, which participate in the conversion of sugars to useful degradation products and energy ATP) in C. glutamicum or which may participate in the production of useful energy-rich molecules ATP) by other processes, such as oxidative phosphorylation. In one embodiment, the SMP molecules participate in the metabolism of carbon compounds such as sugars or the generation of energy molecules ATP) by processes such as oxidative phosphorylation in Corynebacterium glutamicum. In a preferred embodiment, 23 r, the activity of the SMP molecules of the present invention to contribute to carbon Smetabolism or energy production in C. glutamicum has an impact on the production of a desired fine chemical by this organism. In a particularly preferred embodiment, the SMP molecules of the invention are modulated in activity, such that the C. glutamicum metabolic and energetic pathways in which the SMP proteins of the invention participate 00 are modulated in yield, production, and/or efficiency of production, which either directly O or indirectly modulates the yield, production, and/or efficiency of production of a ri desired fine chemical by C glutamicum.

SThe language, "SMP protein" or "SMP polypeptide" includes proteins which are 10 capable of performing a function involved in the metabolism of carbon compounds such as sugars and the generation of energy molecules by processes such as oxidative phosphorylation in Corynebacterium glutamicum. Examples of SMP proteins include those encoded by the SMP genes set forth in Table I and by the odd-numbered SEQ ID NOs. The terms "SMP gene" or "SMP nucleic acid sequence" include nucleic acid sequences encoding an SMP protein, which consist of a coding region and also corresponding untranslated 5' and 3' sequence regions. Examples of SMP genes include those set forth in Table 1. The terms "production" or "productivity" are art-recognized and include the concentration of the fermentation product (for example, the desired fine chemical) formed within a given time and a given fermentation volume kg product per hour per liter). The term "efficiency of production" includes the time required for a.

particular level of production to be achieved (for example, how long it takes for the cell to attain a particular rate of output of a fine chemical). The term "yield" or "product/carbon yield" is art-recognized and includes the efficiency of the conversion of the carbon source into the product fine chemical). This is generally written as, for example, kg product per kg carbon source. By increasing the yield or production of the compound, the quantity of recovered molecules, or of useful recovered molecules of that compound in a given amount of culture over a given amount of time is increased. The terms "biosynthesis" or a "biosynthetic pathway" are art-recognized and include the synthesis of a compound, preferably an organic compound, by a cell from intermediate compounds in what may be a multistep and highly regulated process. The terms "degradation" or a "degradation pathway" are art-recognized and include the breakdown of a compound, preferably an organic compound, by a cell to degradation -24- (NI products (generally speaking, smaller or less complex molecules) in what may be a Smultistep and highly regulated process. The term "degradation product" is artrecognized and includes breakdown products of a compound. Such products may CK themselves have utility as precursor (starting point) or intermediate molecules necessary for the biosynthesis of other compounds by the cell. The language "metabolism" is art- 00 recognized and includes the totality of the biochemical reactions that take place in an organism. The metabolism of a particular compound, then, the metabolism of an Samino acid such as glycine) comprises the overall biosynthetic, modification, and degradation pathways in the cell related to this compound.

S 10 In another embodiment, the SMP molecules of the invention are capable of modulating the production of a desired molecule, such as a fine chemical, in a microorganism such as C glutamicum. There are a number of mechanisms by which the alteration of an SMP protein of the invention may directly affect the yield, production, and/or efficiency of production of a fine chemical from a C. glutamicum strain incorporating such an altered protein. The degradation of high-energy carbon molecules such as sugars, and the conversion of compounds such as NADH and FADH 2 to more useful forms via oxidative phosphorylation results in a number of compounds which themselves may be desirable fine chemicals, such as pyruvate, ATP, NADH, and a number of intermediate sugar compounds. Further, the energy molecules (such as ATP) and the reducing equivalents (such as NADH or NADPH) produced by these metabolic pathways are utilized in the cell to drive reactions which would otherwise be energetically unfavorable. Such unfavorable reactions include many biosynthetic pathways for fine chemicals. By improving the ability of the cell to utilize a particular sugar by manipulating the genes encoding enzymes involved in the degradation and conversion of that sugar into energy for the cell), one may increase the amount of energy available to permit unfavorable, yet desired metabolic reactions the biosynthesis of a desired fine chemical) to occur.

The mutagenesis of one or more SMP genes of the invention may also result in SMP proteins having altered activities which indirectly impact the production of one or more desired fine chemicals from C. glutamicum. For example, by increasing the efficiency of utilization of one or more sugars (such that the conversion of the sugar to useful energy molecules is improved), or by increasing the efficiency of conversion of C1 reducing equivalents to useful energy molecules by improving the efficiency of Soxidative phosphorylation, or the activity of the ATP synthase), one can increase the amount of these high-energy compounds available to the cell to drive normally C1 unfavorable metabolic processes. These processes include the construction of cell walls, transcription, translation, and the biosynthesis of compounds necessary for growth and 00 division of the cells nucleotides, amino acids, vitamins, lipids, etc.) (Lengeler et al.

(1999) Biology of Prokaryotes, Thieme Verlag: Stuttgart, p. 88-109; 913-918; 875-899).

By improving the growth and multiplication of these engineered cells, it is possible to Sincrease both the viability of the cells in large-scale culture, and also to improve their rate of division, such that a relatively larger number of cells can survive in fermentor culture. The yield, production, or efficiency of production may be increased, at least due to the presence of a greater number of viable cells, each producing the desired fine chemical. Further, a number of the degradation and intermediate compounds produced during sugar metabolism are necessary precursors and intermediates for other biosynthetic pathways throughout the cell. For example, many amino acids are synthesized directly from compounds normally resulting from glycolysis or the TCA cycle serine is synthesized from 3-phosphoglycerate, an intermediate in glycolysis). Thus, by increasing the efficiency of conversion of sugars to useful energy molecules, it is also possible to increase the amount of useful degradation products as well.

The isolated nucleic acid sequences of the invention are contained within the genome of a Corynebacterium glutamicum strain available through the American Type Culture Collection, given designation ATCC 13032. The nucleotide sequence of the isolated C. glutamicum SMP DNAs and the predicted amino acid sequences of the C.

glutamicum SMP proteins are shown in the Sequence Listing as odd-numbered SEQ ID NOs and even-numbered SEQ ID NOs, respectively. Computational analyses were performed which classified and/or identified these nucleotide sequences as sequences which encode proteins having a function involved in the metabolism of carbon compounds such as sugars or in the generation of energy molecules by processes such as oxidative phosphorylation in Corynebacterium glutamicum.

The present invention also pertains to proteins which have an amino acid sequence which is substantially homologous to an amino acid sequence of the invention -26the sequence of an even-numbered SEQ ID NO of the Sequence Listing). As used Sherein, a protein which has an amino acid sequence which is substantially homologous to a selected amino acid sequence is least about 50% homologous to the selected amino ,I acid sequence, the entire selected amino acid sequence. A protein which has an amino acid sequence which is substantially homologous to a selected amino acid 00 sequence can also be least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, or 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to the selected amino acid sequence.

IAn SMP protein or a biologically active portion or fragment thereof of the 10 invention can participate in the metabolism of carbon compounds such as sugars or in the generation of energy molecules ATP) by processes such as oxidative phosphorylation in Corynebacterium glutamicum, or can have one or more of the activities set forth in Table 1.

Various aspects of the invention are described in further detail in the following subsections: A. Isolated Nucleic Acid Molecules One aspect of the invention pertains to isolated nucleic acid molecules that encode SMP polypeptides or biologically active portions thereof, as well as nucleic acid fragments sufficient for use as hybridization probes or primers for the identification or amplification of SMP-encoding nucleic acid SMP DNA). As used herein, the term "nucleic acid molecule" is intended to include DNA molecules cDNA or genomic DNA) and RNA molecules mRNA) and analogs of the DNA or RNA generated using nucleotide analogs. This term also encompasses untranslated sequence located at both the 3' and 5' ends of the coding region of the gene: at least about 100 nucleotides of sequence upstream from the 5' end of the coding region and at least about nucleotides of sequence downstream from the 3'end of the coding region of the gene.

The nucleic acid molecule can be single-stranded or double-stranded, but preferably is double-stranded DNA. An "isolated" nucleic acid molecule is one which is separated from other nucleic acid molecules which are present in the natural source of the nucleic acid. Preferably, an "isolated" nucleic acid is free of sequences which naturally flank the nucleic acid sequences located at the 5' and 3' ends of the nucleic acid) in the -27- (N genomic DNA of the organism from which the nucleic acid is derived. For example, in Svarious embodiments, the isolated SMP nucleic acid molecule can contain less than about 5 kb, 4kb, 3kb, 2kb, 1 kb, 0.5 kb or 0.1 kb ofnucleotide sequences which Cl naturally flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived a C. glutamicum cell). Moreover, an "isolated" nucleic acid 00 molecule, such as a DNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or Sother chemicals when chemically synthesized.

A nucleic acid molecule of the present invention, a nucleic acid molecule having a nucleotide sequence of an odd-numbered SEQ ID NO of the Sequence Listing, or a portion thereof, can be isolated using standard molecular biology techniques and the sequence information provided herein. For example, a C. glutamicum SMP DNA can.be isolated from a C. glutamicum library using all or portion of one of the odd-numbered SEQ ID NO sequences of the Sequence Listing as a hybridization probe and standard hybridization techniques as described in Sambrook, Fritsh, E. and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989).

Moreover, a nucleic acid molecule encompassing all or a portion of one of the nucleic acid sequences of the invention an odd-numbered SEQ ID NO:) can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this sequence a nucleic acid molecule encompassing all or a portion of one of the nucleic acid sequences of the invention an odd-numbered SEQ ID NO of the Sequence Listing) can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this same sequence). For example, mRNA can be isolated from normal endothelial cells by the guanidinium-thiocyanate extraction procedure of Chirgwin et al. (1979) Biochemistry 18: 5294-5299) and DNA can be prepared using reverse transcriptase Moloney MLV reverse transcriptase, available from Gibco/BRL, Bethesda, MD; or AMV reverse transcriptase, available from Seikagaku America, Inc., St. Petersburg, FL). Synthetic oligonucleotide primers for polymerase chain reaction amplification can be designed based upon one of the nucleotide sequences shown in the Sequence Listing. A nucleic acid of the invention can be amplified using cDNA or, alternatively, genomic DNA, as a template and -28cN appropriate oligonucleotide primers according to standard PCR amplification techniques. The nucleic acid so amplified can be cloned into an appropriate vector and Scharacterized by DNA sequence analysis. Furthermore, oligonucleotides corresponding cI to an SMP nucleotide sequence can be prepared by standard synthetic techniques, e.g., using an automated DNA synthesizer.

0 In a preferred embodiment, an isolated nucleic acid molecule of the invention 00 O comprises one of the nucleotide sequences shown in the Sequence Listing. The nucleic Sacid sequences of the invention, as set forth in the Sequence Listing correspond to the SCorynebacterium glutamicum SMP DNAs of the invention. This DNA comprises sequences encoding SMP proteins the "coding region", indicated in each oddnumbered SEQ ID NO: sequence in the Sequence Listing), as well as 5' untranslated sequences and 3' untranslated sequences, also indicated in each odd-numbered SEQ ID NO: in the Sequence Listing.. Alternatively, the nucleic acid molecule can comprise only the coding region of any of the sequences in nucleic acid sequences of the Sequence Listing.

For the purposes of this application, it will be understood that each of the nucleic acid and amino acid sequences set forth in the Sequence Listing has an identifying RXA, RXN, or RXS number having the designation "RXA," "RXN," or "RXS" followed by digits RXA01626, RXN00043, or RXS0735). Each of the nucleic acid sequences comprises up to three parts: a 5' upstream region, a coding region, and a downstream region. Each of these three regions is identified by the same RXA, RXN, or RXS designation to eliminate confusion. The recitation "one of the odd-numbered sequences of the Sequence Listing", then, refers to any of the nucleic acid sequences in the Sequence Listing, which may also be distinguished by their differing RXA, RXN, or RXS designations. The coding region of each of these sequences is translated into a corresponding amino acid sequence, which is also set forth in the Sequence Listing, as an even-numbered SEQ ID NO: immediately following the corresponding nucleic acid sequence. For example, the coding region for RXA02735 is set forth in SEQ ID NO:1, while the amino acid sequence which it encodes is set forth as SEQ ID NO:2. The sequences of the nucleic acid molecules of the invention are identified by the same RXA, RXN, or RXS designations as the amino acid molecules which they encode, such that they can be readily correlated. For example, the amino acid sequence designated -29rN RXA00042 is a translation of the coding region of the nucleotide sequence of nucleic dacid molecule RXA00042, and the amino acid sequence designated RXN00043 is a translation of the coding region of the nucleotide sequence of nucleic acid molecule RXN00043. The correspondence between the RXA, RXN and RXS nucleotide and amino acid sequences of the invention and their assigned SEQ ID NOs is set forth in oO Table 1.

0 Several of the genes of the invention are "F-designated genes". An F-designated C gene includes those genes set forth in Table 1 which have an in front of the SRXAdesignation. For example, SEQ ID NO: 11, designated, as indicated on Table 1, as C 10 "F RXA01312", is an F-designated gene, as are SEQ ID NOs: 29, 33, and 39 (designated on Table 1 as "F RXA02803", "F RXA02854", and "F RXA01365", respectively).

In one embodiment, the nucleic acid molecules of the present invention are not intended to include those compiled in Table 2. In the case of the dapD gene, a sequence for this gene was published in Wehrmann, etal. (1998) J. Bacteriol. 180(12): 3159- 3165. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.

In another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleic acid molecule which is a complement of one of the nucleotide sequences of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing), or a portion thereof. A nucleic acid molecule which is complementary to one of the nucleotide sequences of the invention is one which is sufficiently complementary to one of the nucleotide sequences shown in the Sequence Listing the sequence of an odd-numbered SEQ ID NO:) such that it can hybridize to one of the nucleotide sequences of the invention, thereby forming a stable duplex.

In still another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, NI 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least Sabout 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing), C or a portion thereof. Ranges and identity values intermediate to the above-recited ranges, 70-90% identical or 80-95% identical) are also intended to be OO encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended.

to be included. In an additional preferred embodiment, an isolated nucleic acid I molecule of the invention comprises a nucleotide sequence which hybridizes, e.g., 10 hybridizes under stringent conditions, to one of the nucleotide sequences of the invention, or a portion thereof.

Moreover, the nucleic acid molecule of the invention can comprise only a portion of the coding region of the sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, for example a fragment which can be used as a probe or primer or a fragment encoding a biologically active portion of an SMP protein. The nucleotide sequences determined from the cloning of the SMP genes from C. glutamicum allows for the generation of probes and primers designed for use in identifying and/or cloning SMP homologues in other cell types and organisms, as well as SMP homologues from other Corynebacteria or related species. The probe/primer typically comprises substantially purified oligonucleotide. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, preferably about 25, more preferably about 40, 50 or 75 consecutive nucleotides of a sense strand of one of the nucleotide sequences of the invention a sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing), an anti-sense sequence of one of these sequences, or naturally occurring mutants thereof. Primers based on a nucleotide sequence of the invention can be used in PCR reactions to clone SMP homologues. Probes based on the SMP nucleotide sequences can be used to detect transcripts or genomic sequences encoding the same or homologous proteins. In preferred embodiments, the probe further comprises a label group attached thereto, e.g.

the label group can be a radioisotope, a fluorescent compound, an enzyme, or an enzyme co-factor. Such probes can be used as a part of a diagnostic test kit for identifying cells which misexpress an SMP protein, such as by measuring a level of an SMP-encoding -31- N, nucleic acid in a sample of cells, detecting SMP mRNA levels or determining Swhether a genomic SMP gene has been mutated or deleted.

In one embodiment, the nucleic acid molecule of the invention encodes a protein or portion thereof which includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence of an even- O numbered SEQ ID NO of the Sequence Listing) such that the protein or portion thereof maintains the ability to perform a function involved in the metabolism of carbon compounds such as sugars or in the generation of energy molecules ATP) by Iprocesses such as oxidative phosphorylation in Corynebacterium glutamicum. As used 10 herein, the language "sufficiently homologous" refers to proteins or portions thereof which have amino acid sequences which include a minimum number of identical or equivalent an amino acid residue which has a similar side chain as an amino acid residue in a sequence of one of the even-numbered SEQ ID NOs of the Sequence Listing) amino acid residues to an amino acid sequence of the invention such that the protein or portion thereof is able to perform a function involved in the metabolism of carbon compounds such as sugars or in the generation of energy molecules ATP) by processes such as oxidative phosphorylation in Corynebacterium glutamicum.

Protein members of such sugar metabolic pathways or energy producing systems, as described herein, may play a role in the production and secretion of one or more fine chemicals. Examples of such activities are also described herein. Thus, "the function of an SMP protein" contributes either directly or indirectly to the yield, production, and/or efficiency of production of one or more fine chemicals. Examples of SMP protein activities are set forth in Table 1.

In another embodiment, the protein is at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of the invention(e.g., a sequence of an even-numbered SEQ ID NO: of the Sequence Listing).

Portions of proteins encoded by the SMP nucleic acid molecules of the invention are preferably biologically active portions of one of the SMP proteins. As used herein, the term "biologically active portion of an SMP protein" is intended to include a portion, a domain/motif, of an SMP protein that participates in the metabolism of carbon -32- (Nj, compounds such as sugars, or in energy-generating pathways in C. glulamicum, or has ,.an activity as set forth in Table 1. To determine whether an SMP protein or a biologically active portion thereof can participate in the metabolism of carbon N, compounds or in the production of energy-rich molecules in C glutamicum, an assay of enzymatic activity may be performed. Such assay methods are well known to those of OC ordinary skill in the art, as detailed in Example 8 of the Exemplification.

Additional nucleic acid fragments encoding biologically active portions of an SMP protein can be prepared by isolating a portion of one of the amino acid sequences of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing), expressing the encoded portion of the SMP protein or peptide by recombinant expression in vitro) and assessing the activity of the encoded portion of the SMP protein or peptide.

The invention further encompasses nucleic acid molecules that differ from one of the nucleotide sequences of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing) (and portions thereof) due to degeneracy of the genetic code and thus encode the same SMP protein as that encoded by the nucleotide sequences of the invention. In another embodiment, an isolated nucleic acid molecule of the invention has a nucleotide sequence encoding a protein having an amino acid sequence shown in the Sequence Listing an even-numbered SEQ ID In a still further embodiment, the nucleic acid molecule of the invention encodes a full length C.

glutamicum protein which is substantially homologous to an amino acid of the invention (encoded by an open reading frame shown in an odd-numbered SEQ ID NO: of the Sequence Listing).

It will be understood by one of ordinary skill in the art that in one embodiment the sequences of the invention are not meant to include the sequences of the prior art, such as those Genbank sequences set forth in Tables 2 or 4 which were available prior to the present invention. In one embodiment, the invention includes nucleotide and amino acid sequences having a percent identity to a nucleotide or amino acid sequence of the invention which is greater than that of a sequence of the prior art a Genbank sequence (or the protein encoded by such a sequence) set forth in Tables 2 or For example, the invention includes a nucleotide sequence which is greater than and/or at least 58% identical to the nucleotide sequence designated RXA00014 (SEQ ID NO:41), -33a nucleotide sequence which is greater than and/or at least identical to the nucleotide sequence designated RXA00195 (SEQ ID NO:399), and a nucleotide sequence which is greater than and/or at least 42% identical to the nucleotide sequence designated C1 RXA00196 (SEQ ID NO:401). One of ordinary skill in the art would be able to calculate the lower threshold of percent identity for any given sequence of the invention 00 by examining the GAP-calculated percent identity scores set forth in Table 4 for each of O the three top hits for the given sequence, and by subtracting the highest GAP-calculated C percent identity from 100 percent. One of ordinary skill in the art will also appreciate 0that nucleic acid and amino acid sequences having percent identities greater than the CI 10 lower threshold so calculated at least 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more identical) are also encompassed by the invention.

In addition to the C glutamicum SMP nucleotide sequences set forth in the Sequence Listing as odd-numbered SEQ ID NOs, it will be appreciated by those of ordinary skill in the art that DNA sequence polymorphisms that lead to changes in the amino acid sequences of SMP proteins may exist within a population the C.

glutamicum population). Such genetic polymorphism in the SMP gene may exist among individuals within a population due to natural variation. As used herein, the terms "gene" and "recombinant gene" refer to nucleic acid molecules comprising an open reading frame encoding an SMP protein, preferably a C. glutamicum SMP protein. Such natural variations can typically result in 1-5% variance in the nucleotide sequence of the SMP gene. Any and all such nucleotide variations and resulting amino acid polymorphisms in SMP that are the result of natural variation and that do not alter the functional activity of SMP proteins are intended to be within the scope of the invention.

Nucleic acid molecules corresponding to natural variants and non-C. glutamicum homologues of the C. glutamicum SMP DNA of the invention can be isolated based on their homology to the C glutamicum SMP nucleic acid disclosed herein using the C.

glutamicum DNA, or a portion thereof, as a hybridization probe according to standard hybridization techniques under stringent hybridization conditions. Accordingly, in -34- (Nj, another embodiment, an isolated nucleic acid molecule of the invention is at least Snucleotides in length and hybridizes under stringent conditions to the nucleic acid molecule comprising a nucleotide sequence of of an odd-numbered SEQ ID NO: of the C Sequence Listing. In other embodiments, the nucleic acid is at least 30, 50, 100, 250 or more nucleotides in length. As used herein, the term "hybridizes under stringent 00 conditions" is intended to describe conditions for hybridization and washing under which nucleotide sequences at least 60% homologous to each other typically remain hybridized to each other. Preferably, the conditions are such that sequences at least about 65%, more preferably at least about 70%, and even more preferably at least about 10 75% or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to those of ordinary skill in the art and can be found in Current Protocols in Molecular Biology, John Wiley Sons, N.Y. (1989), 6.3.1-6.3.6.

A preferred, non-limiting example of stringent hybridization conditions are hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45°C, followed by one or more washes in 0.2 X SSC, 0.1% SDS at 50-65 0 C. Preferably, an isolated nucleic acid molecule of the invention that hybridizes under stringent conditions to a nucleotide sequence of the invention corresponds to a naturally-occurring nucleic acid molecule. As used herein, a "naturally-occurring" nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature encodes a natural protein). In one embodiment, the nucleic acid encodes a natural C.

glutamicum SMP protein.

In addition to naturally-occurring variants of the SMP sequence that may exist in the population, one of ordinary skill in the art will further appreciate that changes can be introduced by mutation into a nucleotide sequence of the invention, thereby leading to changes in the amino acid sequence of the encoded SMP protein, without altering the functional ability of the SMP protein. For example, nucleotide substitutions leading to amino acid substitutions at "non-essential" amino acid residues can be made in a nucleotide sequence of the invention. A "non-essential" amino acid residue is a residue that can be altered from the wild-type sequence of one of the SMP proteins an even-numbered SEQ ID NO: of the Sequence Listing) without altering the activity of said SMP protein, whereas an "essential" amino acid residue is required for SMP protein activity. Other amino acid residues, however, those that are not conserved or only r, semi-conserved in the domain having SMP activity) may not be essential for activity and Sthus are likely to be amenable to alteration without altering SMP activity.

Accordingly, another aspect of the invention pertains to nucleic acid molecules C, encoding SMP proteins that contain changes in amino acid residues that are not essential for SMP activity. Such SMP proteins differ in amino acid sequence from a sequence of 00 an even-numbered SEQ ID NO: of the Sequence Listing yet retain at least one of the SMP activities described herein. In one embodiment, the isolated nucleic acid molecule comprises a nucleotide sequence encoding a protein, wherein the protein comprises an amino acid sequence at least about 50% homologous to an amino acid sequence of the 10 invention and is capable of participate in the metabolism of carbon compounds such as sugars, or in the biosynthesis of high-energy compounds in C. glutamicum, or has one or more activities set forth in Table 1. Preferably, the protein encoded by the nucleic acid molecule is at least about 50-60% homologous to the amino acid sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, more preferably at least about 70% homologous to one of these sequences, even more preferably at least about 80-90%, 90-95% homologous to one of these sequences, and most preferably at least about 96%, 97%, 98%, or 99% homologous to one of the amino acid sequences of the invention.

To determine the percent homology of two amino acid sequences one of the amino acid sequences of the invention and a mutant form thereof) or of two nucleic acids, the sequences are aligned for optimal comparison purposes gaps can be introduced in the sequence of one protein or nucleic acid for optimal alignment with the other protein or nucleic acid). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in one sequence one of the amino acid sequences the invention) is occupied by the same amino acid residue or nucleotide as the corresponding position in the other sequence a mutant form of the amino acid sequence), then the molecules are homologous at that position as used herein amino acid or nucleic acid "homology" is equivalent to amino acid or nucleic acid "identity"). The percent homology between the two sequences is a function of the number of identical positions shared by the sequences homology of identical positions/total of positions x 100).

-36- CN An isolated nucleic acid molecule encoding an SMP protein homologous to a Sprotein sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) can be created by introducing one or more nucleotide C, substitutions, additions or deletions into a nucleotide sequence of the invention such that one or more amino acid substitutions, additions or deletions are introduced into the O encoded protein. Mutations can be introduced into one of the nucleotide sequences of the invention by standard techniques, such as site-directed mutagenesis and PCRmediated mutagenesis. Preferably, conservative amino acid substitutions are made at Sone or more predicted non-essential amino acid residues. A "conservative amino acid I 10 substitution" is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains lysine, arginine, histidine), acidic side chains aspartic acid, glutamic acid), uncharged polar side chains glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains threonine, valine, isoleucine) and aromatic side chains tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue in an SMP protein is preferably replaced with another amino acid residue from the same side chain family. Alternatively, in another embodiment, mutations can be introduced randomly along all or part of an SMP coding sequence, such as by saturation mutagenesis, and the resultant mutants can be screened for an SMP activity described herein to identify mutants that retain SMP activity. Following mutagenesis of the nucleotide sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, the encoded protein can be expressed recombinantly and the activity of the protein can be determined using, for example, assays described herein (see Example 8 of the Exemplification).

In addition to the nucleic acid molecules encoding SMP proteins described above, another aspect of the invention pertains to isolated nucleic acid molecules which are antisense thereto. An "antisense" nucleic acid comprises a nucleotide sequence which is complementary to a "sense" nucleic acid encoding a protein, e.g., complementary to the coding strand of a double-stranded DNA molecule or -37- CN complementary to an mRNA sequence. Accordingly, an antisense nucleic acid can hydrogen bond to a sense nucleic acid. The antisense nucleic acid can be complementary to an entire SMP coding strand, or to only a portion thereof. In one C embodiment, an antisense nucleic acid molecule is antisense to a "coding region" of the coding strand of a nucleotide sequence encoding an SMP protein. The term "coding region" refers to the region of the nucleotide sequence comprising codons which are 00 Stranslated into amino acid residues the entire coding region of NO. 3 (RXA01626) Scomprises nucleotides 1 to 345). In another embodiment, the antisense nucleic acid N molecule is antisense to a "noncoding region" of the coding strand of a nucleotide sequence encoding SMP. The term "noncoding region" refers to 5' and 3' sequences which flank the coding region that are not translated into amino acids also referred to as 5' and 3' untranslated regions).

Given the coding strand sequences encoding SMP disclosed herein the sequences set forth as odd-numbered SEQ ID NOs in the Sequence Listing), antisense nucleic acids of the invention can be designed according to the rules of Watson and Crick base pairing. The antisense nucleic acid molecule can be complementary to the entire coding region of SMP mRNA, but more preferably is an oligonucleotide which is antisense to only a portion of the coding or noncoding region of SMP mRNA. For example, the antisense oligonucleotide can be complementary to the region surrounding the translation start site of SMP mRNA. An antisense oligonucleotide can be, for example, about 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50 nucleotides in length. An antisense nucleic acid of the invention can be constructed using chemical synthesis and enzymatic ligation reactions using procedures known in the art. For example, an antisense nucleic acid an antisense oligonucleotide) can be chemically synthesized using naturally occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids, phosphorothioate derivatives and acridine substituted nucleotides can be used. Examples of modified nucleotides which can be used to generate the antisense nucleic acid include fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4acetylcytosine, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethyl-2thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-D- -38- CN galactosylqueosine, inosine, N6-isopentenyladenine, 1-methylguanine, 1-methylinosine, S2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, C, methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, 00 uracil-5-oxyacetic acid wybutoxosine, pseudouracil, queosine, 2-thiocytosine, methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5- oxyacetic acid methylester, uracil-5-oxyacetic acid 5-methyl-2-thiouracil, 3-(3-amino-3-N-2- I carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine. Alternatively, the antisense 10 nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).

The antisense nucleic acid molecules of the invention are typically administered to a cell or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encoding an SMP protein to thereby inhibit expression of the protein, by inhibiting transcription and/or translation. The hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule which binds to DNA duplexes, through specific interactions in the major groove of the double helix. The antisense molecule can be modified such that it specifically binds to a receptor or an antigen expressed on a selected cell surface, by linking the antisense nucleic acid molecule to a peptide or an antibody which binds to a cell surface receptor or antigen. The antisense nucleic acid molecule can also be delivered to cells using the vectors described herein. To achieve sufficient intracellular concentrations of the antisense molecules, vector constructs in which the antisense nucleic acid molecule is placed under the control of a strong prokaryotic, viral, or eukaryotic promoter are preferred.

In yet another embodiment, the antisense nucleic acid molecule of the invention is an a-anomeric nucleic acid molecule. An a-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual p-units, the strands run parallel to each other (Gaultier et al. (1987) Nucleic Acids.

Res. 15:6625-6641). The antisense nucleic acid molecule can also comprise a 2'-o- -39- (1N methylribonucleotide (Inoue et al. (1987) Nucleic Acids Res. 15:6131-6148) or a )chimeric RNA-DNA analogue (Inoue et al. (1987) FEBS Lett. 215:327-330).

In still another embodiment, an antisense nucleic acid of the invention is a c ribozyme. Ribozymes are catalytic RNA molecules with ribonuclease activity which are capable of cleaving a single-stranded nucleic acid, such as an mRNA, to which they 00 have a complementary region. Thus, ribozymes hammerhead ribozymes S(described in Haselhoff and Gerlach (1988) Nature 334:585-591)) can be used to C catalytically cleave SMP mRNA transcripts to thereby inhibit translation of SMP SmRNA. A ribozyme having specificity for an SMP-encoding nucleic acid can be C 10 designed based upon the nucleotide sequence of an SMP cDNA disclosed herein SEQ ID NO. 3 (RXA01626)). For example, a derivative of a Tetrahymena L-19 IVS RNA can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in an SMP-encoding mRNA.

See, Cech et al. U.S. Patent No. 4,987,071 and Cech et al. U.S. Patent No.

5,116,742. Alternatively, SMP mRNA can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules. See, Bartel, D. and Szostak, J.W. (1993) Science 261:1411-1418.

Alternatively, SMP gene expression can be inhibited by targeting nucleotide sequences complementary to the regulatory region of an SMP nucleotide sequence an SMP promoter and/or enhancers) to form triple helical structures that prevent transcription of an SMP gene in target cells. See generally, Helene, C. (1991) Anticancer Drug Des. 6(6):569-84; Helene, C. et al. (1992) Ann. N. Y Acad. Sci. 660:27- 36; and Maher, L.J. (1992) Bioassays 14(12):807-15.

B. Recombinant Expression Vectors and Host Cells Another aspect of the invention pertains to vectors, preferably expression vectors, containing a nucleic acid encoding an SMP protein (or a portion thereof). As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a "plasmid", which refers to a circular double stranded DNA loop into which additional DNA segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced bacterial Svectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to 00 which they are operatively linked. Such vectors are referred to herein as "expression O vectors". In general, expression vectors of utility in recombinant DNA techniques are C often in the form ofplasmids. In the present specification, "plasmid" and "vector" can Sbe used interchangeably as the plasmid is the most commonly used form of vector.

C 10 However, the invention is intended to include such other forms of expression vectors, such as viral vectors replication defective retroviruses, adenoviruses and adenoassociated viruses), which serve equivalent functions.

The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, which is operatively linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, "operably linked" is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner which allows for expression of the nucleotide sequence in an in vitro transcription/translation system or in a host cell when the vector is introduced into the host cell). The term "regulatory sequence" is intended to include promoters, enhancers and other expression control elements polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel; Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Regulatory sequences include those which direct constitutive expression of a nucleotide sequence in many types of host cell and those which direct expression of the nucleotide sequence only in certain host cells.

Preferred regulatory sequences are, for example, promoters such as cos-, tac-, trp-, tet-, trp-tet-, Ipp-, lac-, Ipp-lac-, lacI q T7-, T5-, T3-, gal-, trc-, ara-, SP6-, amy, SP02, X-PRor X PL, which are used preferably in bacteria. Additional regulatory sequences are, for example, promoters from yeasts and fungi, such as ADC1, MFa, AC, P-60, CYC I, GAPDH, TEF, rp28, ADH, promoters from plants such as CaMV/35S, SSU, OCS, lib4, -41 CN usp, STLS1, B33, nos or ubiquitin- or phaseolin-promoters. It is also possible to use Sartificial promoters. It will be appreciated by those of ordinary skill in the art that the design of the expression vector can depend on such factors as the choice of the host cell C1 to be transformed, the level of expression of protein desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or 0o peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein SMP proteins, mutant forms of SMP proteins, fusion proteins, etc.).

C The recombinant expression vectors of the invention can be designed for 0expression of SMP proteins in prokaryotic or eukaryotic cells. For example, SMP genes C 10 can be expressed in bacterial cells such as C. glutamicum, insect cells (using baculovirus expression vectors), yeast and other fungal cells (see Romanos, M.A. et al. (1992) "Foreign gene expression in yeast: a review", Yeast 8: 423-488; van den Hondel, C.A.M.J.J. et al. (1991) "Heterologous gene expression in filamentous fungi" in: More Gene Manipulations in Fungi, J.W. Bennet L.L. Lasure, eds., p. 396-428: Academic Press: San Diego; and van den Hondel, C.A.M.J.J. Punt, P.J. (1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, Peberdy, J.F. et al., eds., p. 1-28, Cambridge University Press: Cambridge), algae and multicellular plant cells (see Schmidt, R. and Willmitzer, L. (1988) High efficiency Agrobacterium tumefaciens -mediated transformation ofArabidopsis thaliana leaf and cotyledon explants" Plant Cell Rep: 583-586), or mammalian cells.

Suitable host cells are discussed further in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.

Expression of proteins in prokaryotes is most often carried out with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion proteins. Fusion vectors add a number of amino acids to a protein encoded therein, usually to the amino terminus of the recombinant protein but also to the C-terminus or fused within suitable regions in the proteins. Such fusion vectors typically serve three purposes: 1) to increase expression of recombinant protein; 2) to increase the solubility of the recombinant protein; and 3) to aid in the purification of the recombinant protein by acting as a ligand in affinity purification. Often, in fusion -42- (N expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant protein to enable separation of the recombinant protein from the fusion moiety subsequent to purification of the fusion protein. Such enzymes, C1 and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase.

Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith, OO D.B. and Johnson, K.S. (1988) Gene 67:31-40), pMAL (New England Biolabs, Beverly, 0 MA) and pRIT5 (Pharmacia, Piscataway, NJ) which fuse glutathione S-transferase C (GST), maltose E binding protein, or protein A, respectively, to the target recombinant \0 Sprotein. In one embodiment, the coding sequence of the SMP protein is cloned into a C 10 pGEX expression vector to create a vector encoding a fusion protein comprising, from the N-terminus to the C-terminus, GST-thrombin cleavage site-X protein. The fusion protein can be purified by affinity chromatography using glutathione-agarose resin.

Recombinant SMP protein unfused to GST can be recovered by cleavage of the fusion protein with thrombin.

Examples of suitable inducible non-fusion E. coli expression vectors include pTrc (Amann et al., (1988) Gene 69:301-315), pLG338, pACYC184, pBR322, pUC18, pUC19, pKC30, pRep4, pHS1, pHS2, pPLc236, pMBL24, pLG200, pUR290, pIN- III 113-B 1, Xgtl 1, pBdC1, and pET 1 Id (Studier et al., Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 60-89; and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

Target gene expression from the pTrc vector relies on host RNA polymerase transcription from a hybrid trp-lac fusion promoter. Target gene expression from the pET 11d vector relies on transcription from a T7 gn I0-lac fusion promoter mediated by a coexpressed viral RNA polymerase (T7 gnl). This viral polymerase is supplied by host strains BL21(DE3) or HMS 174(DE3) from a resident X prophage harboring a T7 gnl gene under the transcriptional control of the lacUV 5 promoter. For transformation of other varieties of bacteria, appropriate vectors may be selected. For example, the plasmids pIJ 101, pIJ364, pIJ702 and pIJ361 are known to be useful in transforming Streptomyces, while plasmids pUBI 10, pC194, or pBD214 are suited for transformation of Bacillus species. Several plasmids of use in the transfer of genetic information into Corynebacterium include pHM1519, pBL pSA77, or pAJ667 (Pouwels et al., eds.

(1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

-43r" One strategy to maximize recombinant protein expression is to express the Sprotein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein (Gottesman, Gene Expression Technology: Methods in

C

l Enzymology 185, Academic Press, San Diego, California (1990) 119-128). Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an 00 expression vector so that the individual codons for each amino acid are those 0 preferentially utilized in the bacterium chosen for expression, such as C. glutamicum CN (Wada et al. (1992) Nucleic Acids Res. 20:2111-2118). Such alteration of nucleic acid Ssequences of the invention can be carried out by standard DNA synthesis techniques.

C 10 In another embodiment, the SMP protein expression vector is a yeast expression vector. Examples of vectors for expression in yeast S. cerevisiae include pYepSecl (Baldari, et al., (1987) Embo J. 6:229-234), 2 u, pAG-1, Yep6, Yepl3, pEMBLYe23, pMFa (Kurjan and Herskowitz, (1982) Cell 30:933-943), pJRY88 (Schultz et al., (1987) Gene 54:113-123), and pYES2 (Invitrogen Corporation, San Diego, CA). Vectors and methods for the construction of vectors appropriate for use in other fungi, such as the filamentous fungi, include those detailed in: van den Hondel, C.A.M.J.J. Punt, P.J.

(1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, J.F. Peberdy, et al., eds., p. 1-28, Cambridge University Press: Cambridge, and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York (IBSN 0 444 904018).

Alternatively, the SMP proteins of the invention can be expressed in insect cells using baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells Sf9 cells) include the pAc series (Smith et al.

(1983) Mol. Cell Biol. 3:2156-2165) and the pVL series (Lucklow and Summers (1989) Virology 170:31-39).

In another embodiment, the SMP proteins of the invention may be expressed in unicellular plant cells (such as algae) or in plant cells from higher plants the spermatophytes, such as crop plants). Examples of plant expression vectors include those detailed in: Becker, Kemper, Schell, J. and Masterson, R. (1992) "New plant binary vectors with selectable markers located proximal to the left border", Plant Mol. Biol. 20: 1195-1197; and Bevan, M.W. (1984) "Binary Agrobacterium vectors for plant transformation", Nucl. Acid. Res. 12: 8711-8721, and include pLGV23, pGHlac+, -44r, pBIN19, pAK2004, and pDH51 (Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: j New York IBSN 0 444 904018) In yet another embodiment, a nucleic acid of the invention is expressed in C mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include pCDM8 (Seed, B. (1987) Nature 329:840) and pMT2PC 00 (Kaufman et al. (1987) EMBO J 6:187-195). When used in mammalian cells, the O expression vector's control functions are often provided by viral regulatory elements.

C For example, commonly used promoters are derived from polyoma, Adenovirus 2,

\D

Scytomegalovirus and Simian Virus 40. For other suitable expression systems for both C 10 prokaryotic and eukaryotic cells see chapters 16 and 17 of Sambrook, Fritsh, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.

In another embodiment, the recombinant mammalian expression vector is capable of directing expression of the nucleic acid preferentially in a particular cell type tissue-specific regulatory elements are used to express the nucleic acid). Tissuespecific regulatory elements are known in the art. Non-limiting examples of suitable tissue-specific promoters include the albumin promoter (liver-specific; Pinkert et al.

(1987) Genes Dev. 1:268-277), lymphoid-specific promoters (Calame and Eaton (1988) Adv. Immunol. 43:235-275), in particular promoters of T cell receptors (Winoto and Baltimore (1989) EMBO J. 8:729-733) and immunoglobulins (Banerji et al. (1983) Cell 33:729-740; Queen and Baltimore (1983) Cell 33:741-748), neuron-specific promoters the neurofilament promoter; Byme and Ruddle (1989) PNAS 86:5473-5477), pancreas-specific promoters (Edlund et al. (1985) Science 230:912-916), and mammary gland-specific promoters milk whey promoter; U.S. Patent No. 4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are also encompassed, for example the murine hox promoters (Kessel and Gruss (1990) Science 249:374-379) and the ac-fetoprotein promoter (Campes and Tilghman (1989) Genes Dev. 3:537-546).

The invention further provides a recombinant expression vector comprising a DNA molecule of the invention cloned into the expression vector in an antisense orientation. That is, the DNA molecule is operatively linked to a regulatory sequence in CN a manner which allows for expression (by transcription of the DNA molecule) of an RNA molecule which is antisense to SMP mRNA. Regulatory sequences operatively Slinked to a nucleic acid cloned in the antisense orientation can be chosen which direct C the continuous expression of the antisense RNA molecule in a variety of cell types, for instance viral promoters and/or enhancers, or regulatory sequences can be chosen which 00 direct constitutive, tissue specific or cell type specific expression ofantisense RNA.

The antisense expression vector can be in the form of a recombinant plasmid, phagemid or attenuated virus in which antisense nucleic acids are produced under the control of a N high efficiency regulatory region, the activity of which can be determined by the cell type into which the vector is introduced. For a discussion of the regulation of gene expression using antisense genes see Weintraub, H. et al., Antisense RNA as a molecular tool for genetic analysis, Reviews Trends in Genetics, Vol. 1(1) 1986.

Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms "host cell" and "recombinant host cell" are used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.

A host cell can be any prokaryotic or eukaryotic cell. For example, an SMP protein can be expressed in bacterial cells such as C. glutamicum, insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known to one of ordinary skill in the art. Microorganisms related to Corynebacterium glutamicum which may be conveniently used as host cells for the nucleic acid and protein molecules of the invention are set forth in Table 3.

Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms "transformation" and "transfection", "conjugation" and "transduction" are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid linear DNA or RNA a linearized vector or a gene construct alone without a vector) or nucleic acid in the form of a vector a plasmid, phage, phasmid, phagemid, -46- (N1 transposon or other DNA) into a host cell, including calcium phosphate or calcium Schloride co-precipitation, DEAE-dextran-mediated transfection, lipofection, natural competence, chemical-mediated transfer, or electroporation. Suitable methods for c transforming or transfecting host cells can be found in Sambrook, et al. (Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring 00 Harbor Laboratory Press, Cold Spring Harbor, NY, 1989), and other laboratory manuals.

0 For stable transfection of mammalian cells, it is known that, depending upon the C expression vector and transfection technique used, only a small fraction of cells may \0 0integrate the foreign DNA into their genome. In order to identify and select these 10 integrants, a gene that encodes a selectable marker resistance to antibiotics) is generally introduced into the host cells along with the gene of interest. Preferred selectable markers include those which confer resistance to drugs, such as G418, hygromycin and methotrexate. Nucleic acid encoding a selectable marker can be introduced into a host cell on the same vector as that encoding an SMP protein or can be introduced on a separate vector. Cells stably transfected with the introduced nucleic acid can be identified by, for example, drug selection cells that have incorporated the selectable marker gene will survive, while the other cells die).

To create a homologous recombinant microorganism, a vector is prepared which contains at least a portion of an SMP gene into which a deletion, addition or substitution has been introduced to thereby alter, functionally disrupt, the SMP gene.

Preferably, this SMP gene is a Corynebacterium glutamicum SMP gene, but it can be a homologue from a related bacterium or even from a mammalian, yeast, or insect source.

In a preferred embodiment, the vector is designed such that, upon homologous recombination, the endogenous SMP gene is functionally disrupted no longer encodes a functional protein; also referred to as a "knock out" vector). Alternatively, the vector can be designed such that, upon homologous recombination, the endogenous SMP gene is mutated or otherwise altered but still encodes functional protein the upstream regulatory region can be altered to thereby alter the expression of the endogenous SMP protein). In the homologous recombination vector, the altered portion of the SMP gene is flanked at its 5' and 3' ends by additional nucleic acid of the SMP gene to allow for homologous recombination to occur between the exogenous SMP gene carried by the vector and an endogenous SMP gene in a microorganism. The additional -47- C flanking SMP nucleic acid is of sufficient length for successful homologous recombination with the endogenous gene. Typically, several kilobases of flanking DNA (both at the 5' and 3' ends) are included in the vector (see Thomas, and C Capecchi, M.R. (1987) Cell 51: 503 for a description of homologous recombination vectors). The vector is introduced into a microorganism by electroporation) and O cells in which the introduced SMP gene has homologously recombined with the endogenous SMP gene are selected, using art-known techniques.

In another embodiment, recombinant microorganisms can be produced which Ncontain selected systems which allow for regulated expression of the introduced gene.

For example, inclusion of an SMP gene on a vector placing it under control of the lac operon permits expression of the SMP gene only in the presence of IPTG. Such regulatory systems are well known in the art.

In another embodiment, an endogenous SMP gene in a host cell is disrupted by homologous recombination or other genetic means known in the art) such that expression of its protein product does not occur. In another embodiment, an endogenous or introduced SMP gene in a host cell has been altered by one or more point mutations, deletions, or inversions, but still encodes a functional SMP protein. In still another embodiment, one or more of the regulatory regions a promoter, repressor, or inducer) of an SMP gene in a microorganism has been altered by deletion, truncation, inversion, or point mutation) such that the expression of the SMP gene is modulated. One of ordinary skill in the art will appreciate that host cells containing more than one of the described SMP gene and protein modifications may be readily produced using the methods of the invention, and are meant to be included in the present invention.

A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce express) an SMP protein. Accordingly, the invention further provides methods for producing SMP proteins using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding an SMP protein has been introduced, or into which genome has been introduced a gene encoding a wild-type or altered SMP protein) in a suitable medium until SMP protein is produced. In another -48- N embodiment, the method further comprises isolating SMP proteins from the medium or Sthe host cell.

r, C. Isolated SMP Proteins Another aspect of the invention pertains to isolated SMP proteins, and 00 biologically active portions thereof. An "isolated" or "purified" protein or biologically active portion thereof is substantially free of cellular material when produced by Srecombinant DNA techniques, or chemical precursors or other chemicals when Nchemically synthesized. The language "substantially free of cellular material" includes preparations of SMP protein in which the protein is separated from cellular components of the cells in which it is naturally or recombinantly produced. In one embodiment, the language "substantially free of cellular material" includes preparations of SMP protein having less than about 30% (by dry weight) of non-SMP protein (also referred to herein as a "contaminating protein"), more preferably less than about 20% ofnon-SMP protein, still more preferably less than about 10% of non-SMP protein, and most preferably less than about 5% non-SMP protein. When the SMP protein or biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, culture medium represents less than about 20%, more preferably less than about 10%, and most preferably less than about 5% of the volume of the protein preparation. The language "substantially free of chemical precursors or other chemicals" includes preparations of SMP protein in which the protein is separated from chemical precursors or other chemicals which are involved in the synthesis of the protein. In one embodiment, the language "substantially free of chemical precursors or other chemicals" includes preparations of SMP protein having less than about 30% (by dry weight) of chemical precursors or non-SMP chemicals, more preferably less than about 20% chemical precursors or non-SMP chemicals, still more preferably less than about 10% chemical precursors or non-SMP chemicals, and most preferably less than about 5% chemical precursors or non-SMP chemicals. In preferred embodiments, isolated proteins or biologically active portions thereof lack contaminating proteins from the same organism from which the SMP protein is derived. Typically, such proteins are produced by recombinant expression of, for example, a C. glutamicum SMP protein in a microorganism such as C glutamicum.

-49- An isolated SMP protein or a portion thereof of the invention can participate in Sthe metabolism of carbon compounds such as sugars, or in the production of energy compounds by oxidative phosphorylation) utilized to drive unfavorable metabolic pathways, or has one or more of the activities set forth in Table 1. In preferred embodiments, the protein or portion thereof comprises an amino acid sequence which is 00 sufficiently homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) such that the protein or portion thereof maintains the ability to perform a function involved in the metabolism of carbon Icompounds such as sugars or in the generation of energy molecules by processes such as 10 oxidative phosphorylation in Corynebacterium glutamicum. The portion of the protein is preferably a biologically active portion as described herein. In another preferred embodiment, an SMP protein of the invention has an amino acid sequence set forth as an even-numbered SEQ ID NO: of the Sequence Listing. In yet another preferred embodiment, the SMP protein has an amino acid sequence which is encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, to a nucleotide sequence of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing). In still another preferred embodiment, the SMP protein has an amino acid sequence which is encoded by a nucleotide sequence that is at least about 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to one of the nucleic acid sequences of the invention, or a portion thereof. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. The preferred SMP proteins of the present invention also preferably possess at least one of the SMP activities described herein. For example, a preferred SMP protein of the present invention includes an amino acid sequence encoded by a nucleotide sequence which hybridizes, e.g., hybridizes under stringent conditions, to a nucleotide sequence of the invention, and CFZ which can perform a function involved in the metabolism of carbon compounds such as sugars or in the generation of energy molecules ATP) by processes such as Soxidative phosphorylation in Corynebacterium glutamicum, or which has one or more of C the activities set forth in Table 1.

In other embodiments, the SMP protein is substantially homologous to an amino Sacid sequence of of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing)and retains the functional activity of the protein of one of the Samino acid sequences of the invention yet differs in amino acid sequence due to natural D variation or mutagenesis, as described in detail in subsection I above. Accordingly, in another embodiment, the SMP protein is a protein which comprises an amino acid sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of the invention and which has at least one of the SMP activities described herein. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. In another embodiment, the invention pertains to a full length C. glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention.

Biologically active portions of an SMP protein include peptides comprising amino acid sequences derived from the amino acid sequence of an SMP protein, an amino acid sequence of an even-numbered SEQ ID NO: of the Sequence Listing or the amino acid sequence of a protein homologous to an SMP protein, which include fewer amino acids than a full length SMP protein or the full length protein which is homologous to an SMP protein, and exhibit at least one activity of an SMP protein.

Typically, biologically active portions (peptides, peptides which are, for example, 10, 15, 20, 30, 35, 36, 37, 38, 39, 40, 50, 100 or more amino acids in length) comprise a domain or motif with at least one activity of an SMP protein. Moreover, other -51 CN biologically active portions, in which other regions of the protein are deleted, can be Sprepared by recombinant techniques and evaluated for one or more of the activities described herein. Preferably, the biologically active portions of an SMP protein include CN one or more selected domains/motifs or portions thereof having biological activity.

SMP proteins are preferably produced by recombinant DNA techniques. For 0 example, a nucleic acid molecule encoding the protein is cloned into an expression vector (as described above), the expression vector is introduced into a host cell (as described above) and the SMP protein is expressed in the host cell. The SMP protein 0can then be isolated from the cells by an appropriate purification scheme using standard 10 protein purification techniques. Alternative to recombinant expression, an SMP protein, polypeptide, or peptide can be synthesized chemically using standard peptide synthesis techniques. Moreover, native SMP protein can be isolated from cells endothelial cells), for example using an anti-SMP antibody, which can be produced by standard techniques utilizing an SMP protein or fragment thereof of this invention.

The invention also provides SMP chimeric or fusion proteins. As used herein, an SMP "chimeric protein" or "fusion protein" comprises an SMP polypeptide operatively linked to a non-SMP polypeptide. An "SMP polypeptide" refers to a polypeptide having an amino acid sequence corresponding to an SMP protein, whereas a "non-SMP polypeptide" refers to a polypeptide having an amino acid sequence corresponding to a protein which is not substantially homologous to the SMP protein, a protein which is different from the SMP protein and which is derived from the same or a different organism. Within the fusion protein, the term "operatively linked" is intended to indicate that the SMP polypeptide and the non-SMP polypeptide are fused in-frame to each other. The non-SMP polypeptide can be fused to the N-terminus or C-terminus of the SMP polypeptide. For example, in one embodiment the fusion protein is a GST- SMP fusion protein in which the SMP sequences are fused to the C-terminus of the GST sequences. Such fusion proteins can facilitate the purification of recombinant SMP proteins. In another embodiment, the fusion protein is an SMP protein containing a heterologous signal sequence at its N-terminus. In certain host cells mammalian host cells), expression and/or secretion of an SMP protein can be increased through use of a heterologous signal sequence.

-52- C Preferably, an SMP chimeric or fusion protein of the invention is produced by Sstandard recombinant DNA techniques. For example, DNA fragments coding for the different polypeptide sequences are ligated together in-frame in accordance with conventional techniques, for example by employing blunt-ended or stagger-ended termini for ligation, restriction enzyme digestion to provide for appropriate termini, 00 filling-in of cohesive ends as appropriate, alkaline phosphatase treatment to avoid 0 undesirable joining, and enzymatic ligation. In another embodiment, the fusion gene C1 can be synthesized by conventional techniques including automated DNA synthesizers.

SAlternatively, PCR amplification of gene fragments can be carried out using anchor C1 10 primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and reamplified to generate a chimeric gene sequence (see, for example, Current Protocols in Molecular Biology, Ausubel et al., eds. John Wiley Sons: 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety a GST polypeptide).

An SMP-encoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linked in-frame to the SMP protein.

Homologues of the SMP protein can be generated by mutagenesis, discrete point mutation or truncation of the SMP protein. As used herein, the term "homologue" refers to a variant form of the SMP protein which acts as an agonist or antagonist of the activity of the SMP protein. An agonist of the SMP protein can retain substantially the same, or a subset, of the biological activities of the SMP protein. An antagonist of the SMP protein can inhibit one or more of the activities of the naturally occurring form of the SMP protein, by, for example, competitively binding to a downstream or upstream member of the sugar molecule metabolic cascade or the energy-producing pathway which includes the SMP protein.

In an alternative embodiment, homologues of the SMP protein can be identified by screening combinatorial libraries of mutants, truncation mutants, of the SMP protein for SMP protein agonist or antagonist activity. In one embodiment, a variegated library of SMP variants is generated by combinatorial mutagenesis at the nucleic acid level and is encoded by a variegated gene library. A variegated library of SMP variants can be produced by, for example, enzymatically ligating a mixture of synthetic oligonucleotides into gene sequences such that a degenerate set of potential SMP -53-

C

N sequences is expressible as individual polypeptides, or alternatively, as a set of larger fusion proteins for phage display) containing the set of SMP sequences therein.

There are a variety of methods which can be used to produce libraries of potential SMP C homologues from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA synthesizer, and the 00 synthetic gene then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, in one mixture, of all of the sequences encoding C the desired set of potential SMP sequences. Methods for synthesizing degenerate 0oligonucleotides are known in the art (see, Narang, S.A. (1983) Tetrahedron 39:3; C 10 Itakura et al. (1984) Annu. Rev. Biochem. 53:323; Itakura et al. (1984) Science 198:1056; Ike et al. (1983) Nucleic AcidRes. 11:477.

In addition, libraries of fragments of the SMP protein coding can be used to generate a variegated population of SMP fragments for screening and subsequent selection of homologues of an SMP protein. In one embodiment, a library of coding sequence fragments can be generated by treating a double stranded PCR fragment of an SMP coding sequence with a nuclease under conditions wherein nicking occurs only about once per molecule, denaturing the double stranded DNA, renaturing the DNA to form double stranded DNA which can include sense/antisense pairs from different nicked products, removing single stranded portions from reformed duplexes by treatment with S1 nuclease, and ligating the resulting fragment library into an expression vector. By this method, an expression library can be derived which encodes N-terminal, C-terminal and internal fragments of various sizes of the SMP protein.

Several techniques are known in the art for screening gene products of combinatorial libraries made by point mutations or truncation, and for screening cDNA libraries for gene products having a selected property. Such techniques are adaptable for rapid screening of the gene libraries generated by the combinatorial mutagenesis of SMP homologues. The most widely used techniques, which are amenable to high through-put analysis, for screening large gene libraries typically include cloning the gene library into replicable expression vectors, transforming appropriate cells with the resulting library of vectors, and expressing the combinatorial genes under conditions in which detection of a desired activity facilitates isolation of the vector encoding the gene whose product was detected. Recursive ensemble mutagenesis (REM), a new technique which enhances the -54r- frequency of functional mutants in the libraries, can be used in combination with the dscreening assays to identify SMP homologues (Arkin and Yourvan (1992) PNAS 89:7811-7815; Delgrave et al. (1993) Protein Engineering 6(3):327-331).

In another embodiment, cell based assays can be exploited to analyze a variegated SMP library, using methods well known in the art.

0 D. Uses and Methods of the Invention C The nucleic acid molecules, proteins, protein homologues, fusion proteins, \0 0primers, vectors, and host cells described herein can be used in one or more of the 10 following methods: identification of C. glutamicum and related organisms; mapping of genomes of organisms related to C. glutamicum; identification and localization of C.

glutamicum sequences of interest; evolutionary studies; determination of SMP protein regions required for function; modulation of an SMP protein activity; modulation of the metabolism of one or more sugars; modulation of high-energy molecule production in a cell ATP, NADPH); and modulation of cellular production of a desired compound, such as a fine chemical.

The SMP nucleic acid molecules of the invention have a variety of uses. First, they may be used to identify an organism as being Corynebacterium glutamicum or a close relative thereof. Also, they may be used to identify the presence of C. glutamicum or a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of C. glutamicum genes; by probing the extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a C. glutamicum gene which is unique to this organism, one can ascertain whether this organism is present.

Although Corynebacterium glutamicum itself is nonpathogenic, it is related to pathogenic species, such as Corynebacterium diphtheriae. Corynebacterium diphtheriae is the causative agent of diphtheria, a rapidly developing, acute, febrile infection which involves both local and systemic pathology. In this disease, a local lesion develops in the upper respiratory tract and involves necrotic injury to epithelial cells; the bacilli secrete toxin which is disseminated through this lesion to distal susceptible tissues of the body. Degenerative changes brought about by the inhibition of protein synthesis in these tissues, which include heart, muscle, peripheral nerves, adrenals, kidneys, liver and CN spleen, result in the systemic pathology of the disease. Diphtheria continues to have Shigh incidence in many parts of the world, including Africa, Asia, Eastern Europe and the independent states of the former Soviet Union. An ongoing epidemic of diphtheria C in the latter two regions has resulted in at least 5,000 deaths since 1990.

In one embodiment, the invention provides a method of identifying the presence 0or activity of Cornyebacterium diphtheriae in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention the sequences set forth as odd-numbered or even-numbered SEQ ID NOs, respectively, in Sthe Sequence Listing) in a subject, thereby detecting the presence or activity of C, 10 Corynebacterium diphtheriae in the subject. C. glutamicum and C. diphtheriae are related bacteria, and many of the nucleic acid and protein molecules in C. glutamicum are homologous to C. diphtheriae nucleic acid and protein molecules, and can therefore be used to detect C. diphtheriae in a subject.

The nucleic acid and protein molecules of the invention may also serve as markers for specific regions of the genome. This has utility not only in the mapping of the genome, but also for functional studies ofC. glutamicum proteins. For example, to identify the region of the genome to which a particular C. glutamicum DNA-binding protein binds, the C. glutamicum genome could be digested, and the fragments incubated with the DNA-binding protein. Those which bind the protein may be additionally probed with the nucleic acid molecules of the invention, preferably with readily detectable labels; binding of such a nucleic acid molecule to the genome fragment enables the localization of the fragment to the genome map of C. glutamicum, and, when performed multiple times with different enzymes, facilitates a rapid determination of the nucleic acid sequence to which the protein binds. Further, the nucleic acid molecules of the invention may be sufficiently homologous to the sequences of related species such that these nucleic acid molecules may serve as markers for the construction of a genomic map in related bacteria, such as Brevibacterium lactofermentum.

The SMP nucleic acid molecules of the invention are also useful for evolutionary and protein structural studies. The metabolic and energy-releasing processes in which the molecules of the invention participate are utilized by a wide variety ofprokaryotic and eukaryotic cells; by comparing the sequences of the nucleic acid molecules of the present invention to those encoding similar enzymes from other organisms, the -56- (1,I evolutionary relatedness of the organisms can be assessed. Similarly, such a comparison permits an assessment of which regions of the sequence are conserved and which are not, which may aid in determining those regions of the protein which are essential for C1 the functioning of the enzyme. This type of determination is of value for protein engineering studies and may give an indication of what the protein can tolerate in terms 00 of mutagenesis without losing function.

Manipulation of the SMP nucleic acid molecules of the invention may result in the production of SMP proteins having functional differences from the wild-type SMP Iproteins. These proteins may be improved in efficiency or activity, may be present in 10 greater numbers in the cell than is usual, or may be decreased in efficiency or activity.

The invention provides methods for screening molecules which modulate the activity of an SMP protein, either by interacting with the protein itself or a substrate or binding partner of the SMP protein, or by modulating the transcription or translation of an SMP nucleic acid molecule of the invention. In such methods, a microorganism expressing one or more SMP proteins of the invention is contacted with one or more test compounds, and the effect of each test compound on the activity or level of expression of the SMP protein is assessed.

There are a number of mechanisms by which the alteration of an SMP protein of the invention may directly affect the yield, production, and/or efficiency of production of a fine chemical from a C. glutamicum strain incorporating such an altered protein.

The degradation of high-energy carbon molecules such as sugars, and the conversion of compounds such as NADH and FADH 2 to more useful forms via oxidative phosphorylation results in a number of compounds which themselves may be desirable fine chemicals, such as pyruvate, ATP, NADH, and a number of intermediate sugar compounds. Further, the energy molecules (such as ATP) and the reducing equivalents (such as NADH or NADPH) produced by these metabolic pathways are utilized in the cell to drive reactions which would otherwise be energetically unfavorable. Such unfavorable reactions include many biosynthetic pathways for fine chemicals. By improving the ability of the cell to utilize a particular sugar by manipulating the genes encoding enzymes involved in the degradation and conversion of that sugar into energy for the cell), one may increase the amount of energy available to permit -57- (1N unfavorable, yet desired metabolic reactions the biosynthesis of a desired fine Schemical) to occur.

Further, modulation of one or more pathways involved in sugar utilization C permits optimization of the conversion of the energy contained within the sugar molecule to the production of one or more desired fine chemicals. For example, by 00 reducing the activity of enzymes involved in, for example, gluconeogenesis, more ATP is available to drive desired biochemical reactions (such as fine chemical biosyntheses) N, in the cell. Also, the overall production of energy molecules from sugars may be Imodulated to ensure that the cell maximizes its energy production from each sugar 10 molecule. Inefficient sugar utilization can lead to excess CO 2 production and excess energy, which may result in futile metabolic cycles. By improving the metabolism of sugar molecules, the cell should be able to function more efficiently, with a need for fewer carbon molecules. This should result in an improved fine chemical product: sugar molecule ratio (improved carbon yield), and permits a decrease in the amount of sugars that must be added to the medium in large-scale fermentor culture of such engineered C.

glutamicum.

The mutagenesis of one or more SMP genes of the invention may also result in SMP proteins having altered activities which indirectly impact the production of one or more desired fine chemicals from C. glutamicum. For example, by increasing the efficiency of utilization of one or more sugars (such that the conversion of the sugar to useful energy molecules is improved), or by increasing the efficiency of conversion of reducing equivalents to useful energy molecules by improving the efficiency of oxidative phosphorylation, or the activity of the ATP synthase), one can increase the amount of these high-energy compounds available to the cell to drive normally unfavorable metabolic processes. These processes include the construction of cell walls, transcription, translation, and the biosynthesis of compounds necessary for growth and division of the cells nucleotides, amino acids, vitamins, lipids, etc.) (Lengeler et al.

(1999) Biology of Prokaryotes, Thieme Verlag: Stuttgart, p. 88-109; 913-918; 875-899).

By improving the growth and multiplication of these engineered cells, it is possible to increase both the viability of the cells in large-scale culture, and also to improve their rate of division, such that a relatively larger number of cells can survive in fermentor culture. The yield, production, or efficiency of production may be increased, at least -58- N, due to the presence of a greater number of viable cells, each producing the desired fine Schemical.

Further, many of the degradation products produced during sugar metabolism are N, themselves utilized by the cell as precursors or intermediates for the production of a number of other useful compounds, some of which are fine chemicals. For example, 00 pyruvate is converted into the amino acid alanine, and ribose-5-phosphate is an integral part of, for example, nucleotide molecules. The amount and efficiency of sugar metabolism, then, has a profound effect on the availability of these degradation products in the cell. By increasing the ability of the cell to process sugars, either in terms of 10 efficiency of existing pathways by engineering enzymes involved in these pathways such that they are optimized in activity), or by increasing the availability of the enzymes involved in such pathways by increasing the number of these enzymes present in the cell), it is possible to also increase the availability of these degradation products in the cell, which should in turn increase the production of many different other desirable compounds in the cell fine chemicals).

The aforementioned mutagenesis strategies for SMP proteins to result in increased yields of a fine chemical from C. glutamicum are not meant to be limiting; variations on these strategies will be readily apparent to one of ordinary skill in the art.

Using such strategies, and incorporating the mechanisms disclosed herein, the nucleic acid and protein molecules of the invention may be utilized to generate C. glutamicum or related strains of bacteria expressing mutated SMP nucleic acid and protein molecules such that the yield, production, and/or efficiency of production of a desired compound is improved. This'desired compound may be any product produced by C. glutamicum, which includes the final products of biosynthesis pathways and intermediates of naturally-occurring metabolic pathways, as well as molecules which do not naturally occur in the metabolism of C. glutamicum, but which are produced by a C. glutamicum strain of the invention.

This invention is further illustrated by the following examples which should not be construed as limiting. The contents of all references, patent applications, patents, published patent applications, Tables, and the sequence listing cited throughout this application are hereby incorporated by reference.

2006200985 24 Feb 2006 TABLE 1: GENES IN THE APPLICATION

HMP:

Nucleic Acid SEQ ID NO 3 7

TCA:

Nucleic Acid SEQ ID NO 9 11 13 17 19 21 Amino Acid SEQ ID NO 2 4 6 8 Amino Acid SEQ ID NO 10 12 14 16 18 20 22 Identification Code RXS02735 RXA01626 RXA02245 RXA01 015 Identification Code RXN0 1312 F RXA01312 RXN00231 RXA0131 1 RXAO1 535 RXA00517 RXA01 350 W0074 GR00452 GROO654 GR00290 Coic.

W0082 GR00380 WV0083 GR00380 GROO427 GROO131 GR00392 NT Start INT Stop Function 14576 4270 13639 346 15280 3926 14295 5 6-Phosphoglucolactonase L-ribulose-phosphate 4-epimerase RIBULOSE-PHOSPHATE 3-EPIMERASE (EC: 5.1.3.1) RIBOSE 5-PHOSPHATE ISOMERASE (EC 5.3.1.6) INT Start NT Stop Function 20803 2690 15484 1611 1354 1407 1844 18785 1614 14015 865 2760 2447 2827 SUCCINATE DEHYDROGENASE FLAVOPROTEIN SUBUNIT (EC 1.3.99.1) SUCCINATE DEHYDROGENASE FLAVOPROTEIN SUBUNIT (EC 1.3.99.1) SUCCINATE-SEMIALDEHYDE DEHYDROGENASE (NADP+) (EC 1.2.1.16) SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN (EC 1.3.99.1) FUMARATE HYD)RATASE PRECURSOR (EC 4.2.1.2) MALATE DEHYDROGENASE (EC 1.1.1.37) (EC 1.1.1.82) MALATE DEHYDROGENASE (EC 1.1.1.37) EM B-Pathway Nucleic Amino Acid Acid SEQ SEQ ID NO ID NO 23 24 26 27 28 29 30 31 32 33 34 36 Identification Code ni. NT Start NT Stop Function RXA02149 RXA01 814 RXN02803 F RXA02803 RXN03076 F RXA02854 RXA005I11 GR00639 GROO515 WV0086 GR00784 WV0043 GRI 0002 GROO129 17786 2571 2 1624 1588 1 18754 910 657 400 35 5 513 GLUCOKINASE (EC 2.7.1.2) PHOSPHOGLUCOMUTASE (EC 5.4.2.2) PHOSPHOMANNOMUTASE (EC 5.4.2.8) PHOSPHOGLUCOMUTASE (EC 5.4.2.2)1/ PHOSPHOMANNOMUTASE (EC 5.4.2.8) PHOSPHOGLUCOMUTASE (EC 5.4.2.2)1/ PHOSPHOMANNOMUTASE (EC 5.4.2.8) PHOSPHOGLUCOMUTASE (EC 5.4.2.2)1/ PHOSPHOMANNOMUTASE (EC 5.4.2.8) PHOSPHOGLUCOMUTASE (EC 5.4.2.2) PHOSPHOMANNOMUTASE (EC 5.4.2.8) PHOSPHOGLUCOMUTASE (EC 5.4.2.2)1/ PHOSPHOMANNOMUTASE (EC 5.4.2.8) 2006200985 24 Feb 2006 Table 1 (continued) Nucleic Acid Amino Acid Identification Code Cri. NT Start NT Stop Function SEQ ID NO SEQ ID NO 37 38 RXN01365 W0091 1476 103 PHOSPHOGLUICOMUTASE (EC 5.4.2.2) PH-OSPHOMANNOMULTASE (EC 5.4.2.8) 39 40 F RXA01365 GR00397 897 4 PHOSPHOGLUCOMUTASE (EC 5.4.2.2) PHOSPHOMANNOMUTASE (EC 5.4.2.8) 41 42 RXA00098 GR00014 6525 8144 GLUCOSE-6-PHOSPHATE ISOMERASE (GPI) (EC 5.3.1.9) 43 44 RXA01 989 GR00578 1 630 GLUCOSE-6-PHOSPHATE ISOMERASE A (GPI A) (EC 5.3.1.9) 46 RXA00340 GROO059 1549 2694 PHOSPHOGLYCERATE MUTASE (EC 5.4.2.1) 47 48 RXA02492 GR00720 2201 2917 PHOSPHOGLYCERATE MUTASE (EC 5.4.2.1) 49 50 RXA00381 GR00082 1451 846 PHOSPHOGLYCERATE MUTASE (EC 5.4.2.1) 51 52 RXA02122 GRD0636 6511 5813 PHOSPHOGLYCERATE MUTASE (EC 5.4.2.1) 53 54 RXA00206 GR00032 6171 5134 6-PH-OSPHOIFRUCTOKINASE (EC 2.7.1.11) 56 RXA01243 GR00359 2302 3261 1-PHOSPHOFRUCTOKINASE (EC 2.7.1.56) 57 58 RXA01882 GR00538 1165 2154 1-PH-OSPHOFRUCTOKINASE (EC 2.7.1.56) 59 60 RXA01702 GRO0479 1397 366 FRUCTOSE-BISPHOSPHATE ALDOLASE (EC 4.1.2.13) 61 62 RXA02258 GRD0654 26451 27227 TRIOSEPH-OSPHATE ISOMERASE (EC 5.3. 1.1) 63 64 RXN01225 W0064 6382 4943 GLYCERAILDEHYDE 3-PHOSPH-ATE DEHYDROGENASE (EC 1.2.1.12) 66 IF RXA01225 GRD0354 5302 6741 GLYCERALDEHYDE-3-PHOSPHATE DEHYDROGENASE H-OMOLOG 67 68 RXA02256 GR00654 23934 24935 GLYCERAILDEHYDE 3-PHOSPHATE DEHYDROGENASE (EC 1.2.1.12) 69 70 RXA02257 GR00654 25155 26369 PHOSPHOGLYCERATE KINASE (EC 2.7.2.3) 71 72 RXA00235 GR00036 2365 1091 ENOLASE (EC 4-2.1.11) 73 74 RXA01093 GR00306 1552 122 PYRUVATE KINASE (EC 2.7.1.40) 76 RXN02675 VV0098 72801 70945 PYRUVATE KINASE (EC 2.7.1.40) 77 78 F RXA02675 GR00754 2 364 PYRUVATE KINASE (EC 2.7.1.40) 79 80 IF RXA02695 GR00755 2949 4370 PYRUVATE KINASE (EC 2.7.1.40) 81 82 RXA00682 GR00179 5299 3401 PHOSPHOENOLPYRUVATE SYNTH-ASE (EC 2.7.9.2) 83 84 RXA00683 GR00179 6440 5349 PHOSPHOENOLPYRUVATE SYNTI-ASE (EC 2.7.9.2) 86 RXN00635 VV0135 22708 20972 PYRUVATE DEHYDROGENASE (CYTOCHROME) (EC 1.2.2.2) 87 88 IF RXA02807 GR00788 88 552 PYRUVATE DEHYDROGENASE (CYTOCHROME) (EC 1.2.2.2) 89 90 F RXA00635 GRO0167 3 923 PYRUVATE DEHYDROGENASE (CYTOCHROME) (EC 1.2.2.2) 91 92 RXN03044 W0019 1391 2221 PYRUVATE DEHYDROGENASE El COMPONENT (EC 1.2.4.1) 93 94 F RXA02852 GR00852 3 281 PYRUVATE DEHYDROGENASE El COMPONENT (EC 1.2.4.1) 96 F RXA00268 GROO041 125 955 PYRUVATE DEHYDROGENASE El COMPONENT (EC 1.2.4.1) 97 98 RXN03086 W0049 2243 2650 PYRUVATE DEHYDROGENASE El COMPONENT (EC 1.2.4.1) 99 100 F RXA02887 GRI0022 411 4 PYRUVATE DEHYDROGENASE El COMPONENT (EC 1.2.4.1) 101 102 RXN03043 VVO019 1 1362 PYRUVATE DEHYDROGENASE El COMPONENT (EC 1.2.4.1) 103 104 F RXA02897 GR10039 1291 5 PYRUVATE IDEHYDROGENASE El COMPONENT (EC 1.2.4.1) 105 106 RXN03083 WV0047 88 1110 DIHYDROLIPOAMIDE DEHYDROGENASE (EC 1.8.1.4) 107 108 F RXA02853 GR10001 89 1495 DIHYOROLIPOAMIDE DEHYDROGENASE (EC 1.8.1.4) 109 110 RXA02259 GR00654 27401 30172 PHOSPHOENOLPYRULVATE CARBOXYLASE (EC 4.1.1.31) 11111 112 RXN02326 W0047 4500 5315 PYRUVATE CARBOXYLASE (EC 6.4. 1.1) 113 114 IF RXA02326 GR00668 5338 4523 PYRUVATE CARBOXYLASE 115 116 RXN02327 W0047 3533 4492 PYRUVATE CARBOXYLASE (EC 6.4.1.1) 117 118 IF RXA02327 GR00668 6305 5346 PYRUVATE CARBOXYLASE 119 120 RXN02328 WV0047 1842 3437 PYRUVATE CARBOXYLASE (EC 6.4.1.1) 121 122 F RXA02328 GR00668 7783 6401 PYRUVATE CARBOXYLASE (EC 6.4.1.1) 123 124 RXN01048 WV0079 12539 11316 MAILIC ENZYME (EC 11.1.39) 2006200985 24 Feb 2006 Table 1 (continued) NT Start NT Stop Function Nucleic Acid SEQ ID NO 125 127 129 131 133 135 137 139 141 143 145 147 149 151 153 155 Amino Acid SEQ ID NO 126 128 130 132 134 136 138 140 142 144 146 148 150 152 154 156 Identification Code F RXA01 048 F RXA00290 RXA02694 RXN00296 F RXA00296 RXA01901 RXN01 952 F RXA01952 F RXA0i955 RXA00293 RXN01 130 F RXAO1 130 RXN031 12 F RXA01 133 RXN00871 F RXA00871 Cig.

GR00296 GR00046 GR00755 W0176 GR00048 GRO0544 W0105 GR00562 GR00562 GRO0047 W0157 GR00315S W0085 GR0031 6 W0127 GR00239 W0354 GRCO81 6 W001 9 GROO422 GROO211 W0213 GR00690 W0098 W0052 W0377 W0382 W00g8 W0009 W0009 3.

4693 1879 35763 3 4158 9954 4611 2645 6138 2 509 58 3127 2344 287 287 7474 1250 3993 6135 1390 59053 3216 310 3 14370 3477 3703 290 5655 2820 38606 2837 5417 11666 216 6209 1734 5536 304 6 1116 2240 3207 559 562 8298 2074 2989 5224 686 58385 3428 519 281 12541 2296 3533 MALIC ENZYME (EC 1. 1. 1.39) MALIC ENZYME (EC 1. 1. 1.39) L-LACTATE DEH-YDROGENASE (EC 1.1.1.27) D-LACTATE DEHYOROGENASE (CYTOCHROME) (EC 1.1.2.4) 0-LACTATE DEHYDROGENASE (CYTOCHROME) (EC 1.1.2.4) L-LACTATE DEHYDROGENASE (CYTOCI-ROME) (EC 1.1.2.3) 0-LACTATE DEI-YDROG ENASE (EC 1. 1.1.-28) D-LACTATE DIEH-YDROGENASE (EC 1.1.1.28) D-LACTATE DIEHYDROGENASE (EC 1.1.1.28) D-3-PH-OSPHOGLYCERATE DEHYDROGENASE (EC 1A1.1.95) 0-3-PHOSPHOGLYCERATE DEHYDROGENASE (EC 1.1.1.95) D-3-PH-OSPHOGLYCERATE DEHYDROGENASE (EC 1.1.1.95) 0-3-PHOSPHOGLYCERATE DEHYDROGENASE (EC 1.1.1.95) D-3-PHOSPHOGLYCERATE DEHYDROGENASE (EC 1.1.1.95) IOLB PROTEIN IOLB PROTEIN: 0-FRUCTOSE 1 .6-BISPHOSPHATE GLYCERONE-CC PHOSPHATE +0D- GLYCERALDEHYDE 3-PHOSPHATE.

IOLS PROTEIN IOLS PROTEIN NAGD PROTEIN PUTATIVE N-GLYCERALDEHYDE-2-PHOSPHOTRANSFERASE GLPX PROTEIN 0-3-PHOSPHOGLYCERATE DEHYDROGENASE (EC 1.1.1.95) 0-3-PHOSPHOGLYCERATE DEHYDROGENASE (EC 1.1.1.95) PHOSPHOGLYCERATE MUTASE (EC 5.4.2.1) PYRUVATE CARBOXYLASE (EC 6.4.1.1) PYRUVATE DEHYDROGENASE El COMPONENT (EC 1.2.4.1) PYRUVATE DIEHYDROGENASE El COMPONENT (EC 1.2.4.1) PHOSPHOENOLPYRUVATIE CARBOXYKINASE [GTPJ (EC 4.1.1.32) LIPOAMIDE DEHYDROGENASE COMPONENT (E0) OF BRANCHED- CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX (EC 1.8. 1.4) LIPOAMIDE DEHYDROGENASE COMPONENT (E3) OF BRANCHED- CHAIN ALPHA-KIETO ACID DEHYDROGENASE COMPLEX (EC 1.8.1.4) RXN02829 F RXA02829 RXN01468 F RXA01468.

RXA00794 RXN02920 F RXA02379 RXN02688 RXN03087 RXN03 186 RXN03187 RXN02591 RXS01 260 RXS01 261 183 184 Glycerol metabolism Nucleic Acid SEQ ID NO 185 187 189 191 193 195 Amino Acid Identification Code SEQ ID NO 186 RXA02640 188 RXN01025 190 F RXAOIO25 192 RXA01851 194 RXA01242 196 RXA02288 Contig GR00749 W0143 GR00293 GRO0525 GR00359 GROO661 NT Start NT Stop Function 1400 5483 939 3515 1526 992 GLYCEROL KINASE (EC 2.7.1.30) GLYCEROL-3-PHOSPHATE DEI-YI)ROGENASE (NAD(P)i-) (EC 1.1.1.94) GLYCEROL-3-PHOSPHATE DEHYDROGENASE (EC 1.1.1.94) AEROBIC GLYCEROL-3-PHOSPH-ATE DEHYDROGENASE (EC 1.1.99.5) GLYCEROL-3-PHOSPHATE REGULON REPRESSOR GLYCEROL-3-PHOSPHATE REGULON REPRESSOR 2006200985 24 Feb 2006 Table I (continued) NT Start NT Stop Function Nucleic Acid AminoAcid Identification Code SEQ ID NO SEQ ID NO 197 198 RXN01891 Contig.

199 200 201 202 203 204 F RXA01891 RXA02414 RXN01 580 W01 22 24949 24086 GLYCEROL-3-PHOSPHATE-BINDING PERIPLASMIC PROTEIN

PRECURSOR

GROO541 1736 918 GLYCEROL-3-PHOSPHATE-BINDING PERIPLASMIC PROTEIN

PRECURSOR

GR00703 3808 3062 Uncharacterized protein involved in glycerol metabolism (homolog of Drosophila rhomboid) W0122 22091 22807 Glycerophospharyl diester phosphodiesterase Acetate metabolism Nucleic Acid Amino Acid Identification Code SEQ ID NO SEQ ID NO 205 2D6 RXA01436 207 208 RXA00686 209 210 RXA00246 211 212 RXA01571 213 214 RXA01572 215 216 RXA01758 217 218 RXA02539 219 220 RXN03061 221 222 RXN03150 223 224 RXN01 340 225 226 RXN01498 227 228 RXN02674 229 230 RXN00868 231 232 RXN01 143 233 234 RXN01 146 235 236 RXN01 144 Co~nti NT Start NT Stop Function GRO0418 2547 1357 ACETATE KINASE (EC 2.7.2.1) GR00179 8744 7941 ACETATE OPERON REPRESSOR GR00037 4425 3391 ALCOHOL DEHYDROGENASE (EC 1.1.1.1) GR00438 1360 1959 ALCOHOL DEHYOROGENASE (EC 1.1.1.1) GR00438 1928 2419 ALCOHOL DEHYDROGENASE (EC 1.1.1.1) GR00498 3961 2945 ALCOHOL DEHYDROGENASE (EC 1.1.1.1) GR00726 11676 10159 ALDEHYDE DEH-YDROGENASE (EC WV0034 108 437 ALDEHYDE DEHYDROGENASE (EC 1.2.1.3) WV0155 10678 10055 ALDEHYDE DEHYDROGENASE (EC 1.2.1.3) WV0033 3 860 ALDEHYDE DEHYDROGENASE (EC 1.2.1.3) WV0008 1598 3160 ALDEHYDE DEHYDROGENASE (EC 1.2.1.3) WV0315 15614 14163 ALDEHYDE DEHYDROGENASE (EC 1.2.1.3) W0127 2230 320 ACETOLACTATE SYNTHASE LARGE SUBUNIT (EC 4.1.3.18) WV0077 9372 8254 ACETOLACTATE SYNTHASE LARGE SUBUNIT (EC 4.1.3.18) W0264 243 935 ACET0LACTATE SYNTHASE LARGE SUBUNIT (EC 4.1.3.18) WV0077 8237 7722 ACETOLACTATE SYNTHASE SMALL SUBUNIT (EC 4.1.3.18) Butanediol, diacetyl and acetoin formation Nucleic Acid Amino Acid Identification Code Cot.

SEQ ID NO SEQ ID NO 237 238 RXA02474 GRO07 239 240 RXA02453 GRO07 241 242 RXS01758 W01 11 NT Start NT Stop Function 15 8082 7309 (S,S)-butane-2,3-diol dehydrogenase (EC 1.1.1.76) 10 6103 5351 ACETOIN(DIACETYL) REDUCTASE (EC 1.1.1.5) 27383 28399 ALCOHOL DEHYDROGENASE (EC 1. 1.1. 1) 2006200985 24 Feb 2006 HMP-Cycle Table 1 (continued) NT Start NT Stop Function Nucleic Acid SEQ ID NO Amino Acid Identification Code SEQ ID NO 243 244 RXA02737 245 246 RXA02738 247 248 RXA02739 249 250 RXA00965 251 252 RXN00999 922kig GR00763 GR00763 GR00763 GR00270 W0106 GR00283 3312 4499 6769 1232 2817 3012 1771 GLUCOSE-6-PH-OSPHATE 1 -DEHYDROGENASE (EC 1.1.1.49) 3420 TRANSALDOLASE (EC 2.2.1.2) 4670 TRANSKETOLASE (EC 2.2.1.1) 510 6-PHOSPHOGLUCONATE DEHYDROGENASE, DECARBOXYLATING (EC 1.1.1.44) 1366 6-PHOSPHOGLUCONATE DEHYDROGENASE, DECARBOXYLATING (EC 1.1.1.44) 4448 6-PHOSPHOGLUCONATE DEHYDROGENASE, DECARBOXYLATING (EC 1.I.1.44) 253 254 F RXA00999 Nucleotide sugar conversion Nucleic Acid SEQ ID NO 255 257 259 261 263 265 267 269 271 273 275 277 279 281 283 285 Amino Acid SEQ ID NO 256 258 260 262 264 266 268 270 272 274 276 278 280 282 284 286 Identification Code RXN02596 F RXA02596 F RXA02642 RXA02572 RXA02485 RXA01 216 RXA0 1259 RXA02028 RXA01 262 RXA01 377 RXA02063 RXN00014 F RXA00014 RXA 1 570 RXA02666 RXA00825 GR00742 GR00749 GR00737 GR00718 GR00352 GROO367 GR00616 GR00367 GR0O400 GR00626 W0048 GROO002 GROO438 GR00753 GR00222 NT Start NT Stop Function 48784 5383 2 2345 2302 987 573 8351 3935 3301 8848 4448 427 7260 222 47582 489 5880 646 3445 1202 130 998 7191 5020 4527 9627 5227 1281 6493 1154 UDP-GALACTOPYRANOSE MUTASE (EC 5.4.99.9) UDP-GALACTOPYRANOSE MUTASE (EC 5.4.99.9) UDP-GALACTOPYRANOSE MUTASE (EC 5.4.99.9) UDP-GLUCOSE 6-DEHYDROGENASE (EC 1.1.1.22) UDP-N-ACETYLENOLPYRUVOYLGLUCOSAMINE REDUCTASE (EC 1.1.1.158) UDP-N-ACETYLGLUCOSAMINE PYROPHOSPHORYLASE (EC 2.7.7.23) UTP-GLUCOSE-1 -PHOSPHATE URIDYLYLTRANSFERASE (EC 2.7.7.9) UTP-GLUCOSE-1 -PHOSPHATE URIDYLYLTRANSFERASE (EC 2.7.7.9) GDP-MANNOSE 6-DEHYDROGENASE (EC 1.1.1.132) MANNOSE-1 -PHOSPHATE GUANYLTRANSFERASE (EC 2.7.7.13) GLUCOSE-i -PHOSPHATE ADENYLYLTRANSFERASE (EC 2.7.7.27) GLUCOSE-i -PHOSPHATE THYMIDYLYLTRANSFERASE (EC 2.7.7.24) GLUCOSE-I -PHOSPHATE THYMIDYLYLTRANSFERASE (EC 2.7.7.24) GLUCOSE-i -PHOSPHATE THYMIDYLYLTRANSFERASE (EC 2.7.7.24) D-RIBITOL-5-PHOSPNATE CYTIDYLYLTRANSFERASE (EC 2.7.7.40) DTDP-GLUCOSE 4,6-DEHYDRATASE (EC 4.2.1.46) Inositol and ribitol metabolism Nucleic Acid Amino Acid SEQ ID NO SEQ ID NO 287 288 Identification Code Contig. NT Start NT Stop Function RXA01887 XA187 GROO539 4219 3209 MYO-INOSITOL 2-DEHYDROGENASE (EC 1.1. 1.18) 2006200985 24 Feb 2006 Table 1 (continued) Nucleic Acid Amino Acid Identification Code Cni. NT Start NT Stop Function SEQ 10 NO SEQ ID NO 289 290 RXN00013 W0048 7966 8838 MYO-INOSITOL-11 (OR 4)-MONOPHOSP-ATASE 1 (EC 3.1.3.25) 291 292 F R.XAOOOI13 GR00002 3566 4438 MYO-INOSITOL-1 (OR 4)-MONOPHOSPHATASE 1 (EC 3.1.3.25) 293 294 RXA01099 GR00306 6328 5504 INOSITOL MONOPI-OSPHATE PHOSPHATASE 295 296 RXN01332 W0273 579 4 MYO-INOSITOL 2-DEHYDROGENASE (EC 1.1.1.18) 297 298 F RXA01332 GR00388 552 4 MYO-INOSITOL 2-DEH-YDROGENASE (EC1.1.) 299 300 RXA01632 GRO0454 2338 3342 MYO-INOSITOL 2-DEHYDROGENASE (EC 1.1.1.18) 301 302 RXA01633 GRO0454 3380 4462 MYO-INOSITOL 2-DEHYDROGENASE (EC 1.1.1.18) 303 304 RXN01406 WV0278 2999 1977 MYO-INOSITOL 2-DEHYDROGENASE (EC1.118 305 306 RXN01630 W0050 48113 47037 MYO-INOSITOL 2-DEHYDROGENASE (EC 1.1. 1. 18) 307 308 RXN00528 W0079 23406 22318 MYO-INOSITOL-1 -PHOSPHATE SYNTHASE (EC 5.5.1.4) 309 310 RXN03057 WV0028 7017 7688 MYO-INOSITOL 2-DEHYDROGENASE (EC 1.1.1.18) 311 312 F RXA02902 GR10040 10277 10948 GLUCOSE-FRUCTOSE OXIDOREDUCTASE PRECURSOR (EC 1.1.99.28) 313 314 RXA00251 GROO038 931 224 RIBITOL 2-DEHYDROGENASE (EC 1.1.1.56) Utilization of sugars Nucleic Acid Amino Acid Identification Code SEQ ID NO SEQ ID NO 315 316 RXN02654 317 318 F RXA02654 319 320 RXN01049 321 322 F RXA01O49 323 324 F RXA01050 325 326 RXA00202 327 328 RXN00872 329 330 F RXA00872 331 332 RXN00799 333 334 F RXA00799 Conlik NT Start NT Stop Function W0090 12206 13090 GLUCOSE 1-DEHYDROGENASE (EC 1.1.1.47) GR00752 7405 8289 GLUCOSE 1-DEHYDROGENASE 11 (EC 1.1.1.47) W0079 9633 11114 GLUCONOKINASE (EC 2.7.1.12) GR00296 1502 492 GLUCONOKINASE (EC 2.7.1.12) GR00296 1972 1499 GLUCONOKINASE (EC 2.7.1.12) GR00032 1216 275 0-RIBOSE-BINDING PERIPLASMIC PROTEIN PRECURSOR W0127 6557 5604 FRUCTOKINASE (EC 2.7.1.4) GR00240 565 108a6 FRUCTOKINASE (EC 2.7.1.4) W0009 58477 56834 PERIPLASMIC BETA-GLUCOSIDASEIBETA-XYLOSIDASE PRECURSOR (EC 3.2.1.21) (EC 3.2.1.37) GR00214 1 1584 PERIPLASMIC BETA-GLUCOSIDASEIBETA-XYLOSIDASE PRECURSOR (EC 3.2.1.21) (EC 3.2.1.37) GROO003 12028 10520 MANNITOL 2-DEHYDROGENASE (EC 1.1.1.67) GR00725 6880 7854 FRUCTOSE REPRESSOR W0006 7035 8180 Hypothetical Oxidareductase (EC 111- GROD053 316 5 GLUCOSE-FRUCTOSE OXIDOREDUCTASE PRECURSOR (EC 1.1.99.28) W0006 6616 7050 GLUCOSE--FRUCTOSE OXIDOREDUCTASE PRECURSOR (EC 1.1. 99.28) GRO0053 735 301 GLUCOSE-FRUCTOSE OXIDOREDUCTASE PRECURSOR (EC 1.1.99.28) GRO0007 1246 5 SUCROSE-6-PHOSPHATE HYOROLASE (EC 3.2.1.26) GR0O615 725 6 SLICROSE-6-PHOSPHATE HYDROLASE (EC 3.2.1.26) GR00626 1842 349 SUCROSE-6-PHOSPH-ATE HYDROLASE (EC 3.2.1.26) RXA00032 RXA02528 RXN0031 6 F RXA00309 RXN00310 343 344 345 346 F RXA00310 RXA00O41 RXA02026 RXA02061 2006200985 24 Feb 2006 Table I (continued) Nucleic Acid Amino Acid Identification Code Cni. NT Start NT Stop Function SEQ ID NO SEQ ID NO 353 354 RXN01369 WV01 24 595 1776 MANNOSE-6-PHOSPH-ATE ISOMERASE (EC 5.3.1.8) 355 356 F RXA01 369 GR00398 3 503 MANNOSE-6-PHOSPHATE ISOMERASE (EC 5.3.1.8) 357 358 F RXA0 1373 GR00399 595 1302 MANNOSE-6-PHOSPHATE ISOMERASE (EC 5.3.1.8) 359 360 RXA02611 GR00743 1 1752 1,4-AIPHA-GLUCAN BRANCHING ENZYME (EC 2.4.11.118) 361 362 RXA02612 GR00743 1793 3985 1,4-ALPHA-GLUCAN BRANCHING ENZYME (EC 2.4.1.18) 363 364 RXN01 854 VV0184 1 1890 GLYCOGEN DEBRANCHING ENZYME (EC 2.4.1.25) (EC 3.2.1.33) 365 366 F RXA01884 GR00539 3 1475 GLYCOGEN DEBRANCHING ENZYME (EC 2.4.1.25) (EC 3.2.1.33) 367 366 RXA01 111 GR00306 16981 17427 GLYCOGEN OPERON PROTEIN GLGX (EC 3.2. 369 370 RXN01 550 W0143 14749 16260 GLYCOGEN PHOSPHORYLASE (EC 2.4.1.1) 371 372 F RXA01550 GR00431 3 1346 GLYCOGEN PHOSPHORYLASE (EC 2.4.1.1) 373 374 RXN02100 WV031 8 2 2326 GLYCOGEN PHOSPHORYLASE (EC 2.4.1.1) 375 376 F RXA02100 GR00631 3 920 GLYCOGEN PI-OSPHORYLASE (EC; 2.4.1.1) 377 378 F RXA02113 GR00633 2 1207 GLYCOGEN PHOSPHORYLASE (EC 2.4.1.1) 379 380 RXA02147 GRO0639 15516 16532 ALPHA-AMYLASE (EC 3.2.1.1) 381 382 RXA01 478 GRO0422 10517 12352 GLUCOAMYLASE G1 AND G2 PRECURSOR (EC 3.2.1.3) 383 384 RXA01888 GR00539 4366 4923 GLUCOSE-RESISTANCE AMYLASE REGULATOR 385 386 RXN01927 WV0127 50623 49244 XYLULOSE KINASE (EC 2.7.1.17) 387 388 F RXA01927 GR00555 3 1118 XYLULOSE KINASE (EC 2.7.1.17) 389 390 RXA02729 GR00762 747 4 RIBOKINASE (EC 2.7.1.15) 391 392 RXA02797 GR00778 1739 2641 RIBOKINASE (EC 2.7.1.15) 393 394 RXA02730 GR00762 1768 731 RIBOSE OPERON REPRESSOR 395 396 RXA02551 GR00729 2193 2552 6-PHOSPHO-BETA-GLUCOSIDASE (EC 3.2.1.86) 397 398 RXA01 325 GR00385 5676 5005 DEOXYRIBOSE-PHOSPHATE ALDOLASE (EC 4.1.2.4) 399 400 RXA00195 GROO030 543 1103 1-deoxy-D-xytu lose 5-phosphate reductoisomerase (EC 401 402 RXA00196 GROO030 1094 1708 1-deoxy-D-xylulose 5-phosphate reduotoisomerase (EC 403 404 RXNO1 562 WV0191 1230 3137 1-DEOXYXYLULOSE-5-PHOSPHATE SYNTHASE 405 406 F RXA01562 GR00436 2 1039 1-DEOXYXYLULOSE-5-PHOSPHATE SYNTHASE 407 408 F RXA01705 GROO480 971 1573 1-DEOXYXYLULOSE-5-PH-OSPH-ATE SYNTI-ASE 409 410 RXN00879 WV0099 8763 6646 4-ALPHA-GLUCANOTRANSFERASE (EC 2.4.1.25) 411 412 F RXA00879 GR00242 5927 3828 4-ALPHA-GLUCANOTRANSFERASE (EC 2.4.1.25). amnylomnaltase 413 414 RXN00043 W01119 3244 2081 N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE (EC 3.5.1.25) 415 416 F RXA00043 GROO007 3244 2081 N-ACETYLGLUCOSAMINE-6-PHOSPHATE DEACETYLASE (EC 3.5.1.25) 417 418 RXN01752 VV0127 35265 33805 N-ACETYLGLUCOSAMINYLTRANSFERASE (EC 419 420 F RXA01 839 GR00520 1157 510 N-ACETYLGLUCOSAMINYLTRANSFERASE (EC 421 422 RXA01859 GR00529 1473 547 N-ACETYLGILUCOSAMINYLTRANSFE RASE (EC 423 424 RXAO42 GROD007 2037 1279 GLUCOSAMINE-6-PHOSPHATE ISOMERASE (EC 5.3.1.10) 425 426 RXA01 482 GR00422 17271 15397 GLUCOSAMINE-FRUCTOSE-6-PHOSPHATE AMINOTRANSFERASE (ISOMERIZING) (EC 2.6.1.16) 427 428 RXN03179 WV0336 2 667 URONATE ISOMERASE (EC 5.3.1.12) 429 430 F RXA02872 GR10013 675 4 URONATE ISOMERASE, Glucuronate isomerase (EC 5.3.1.12) 431 432 RXN031 80 W0337 672 163 URONATE ISOMERASE (EC 5.3.1.12) 433 434 F RXA02873 GR10014 672 163 URONATE ISOMERASE, Glucuronate isomerase (EC 5.3.1.12) 435 436 RXA02292 GR00662 1611 2285 GALACTOSIDE O-ACIETYLTRANSFERASE (EC 2.3.1.18) 437 438 RXA02666 GR00753 7260 64 -93 D-RIBITOL-5-PHOSPHATE CYTIDYLYLTRANSFERASE (EC 2.7.7.40) 439 440 RXA00202 GR00032 1216 275 D-RIBOSE-BINDING PERIPLASMIC PROTEIN PRECURSOR 441 442 RXA02440 GR00709 5097 4258 0-RIBOSE-BINDING PERIPLASMIC PROTEIN PRECURSOR 2006200985 24 Feb 2006 Table 1 (continued) Nucleic Acid Amino Acid Identification Code Contig. NT Stat NT Stop Function SEQ ID NO SEQ IDNO 443 444 RXN01569 WV0009 41086 42444 dTDP-4-OEHYDRORHAMNOSE REDUCTASE (EC 1.1.1.133) 445 446 F RXA011 569 GRO0438 2 427 DTDP-4-DEHYDRORHAMNOSE REDUCTASE (EC 1.1.1.133) 447 448 F RXA02055 GROO624 7122 8042 DTDP-4-DEHYDRORHAMNOSE REDUCTASE (EC 1.1.1.133) 449 450 RXA00825 GR00222 222 1154 DTOP-GLUCOSE 4,6-DEHYDRATASE (EC 4.2.1.46) 451 452 RXA02054 GR00624 6103 7119 OTOP-GLUCOSE 4,6-DEHYDRATASE (EC 4.2.1.46) 453 454 RXN00427 WV01 12 7004 6219 dTDP-RHAMNOSYL TRANSFERASE RFBF (EC 455 456 F RXA00427 GRO0098 1591 2022 DTDP-RH-AMNOSYL TRANSFERASE RFBF (EC 457 458 RXA00327 GR00057 10263 9880 PROTEIN ARAJ 459 460 RXA00328 GR00057 11147 10656 PROTEIN ARAJ 461 462 RXA00329 GR00057 12390 11167 PROTEIN ARMJ 463 464 RXN01 554 W01 35 28686 26545 GLUCAN ENOO-1,3-BETA-GLUCOSIOASE Al PRECURSOR (EC 3.2.1.39) 465 466 RXN03015 W0063 289 8 UDP-GLUCOSE 6-DEHYDROGENASE (EC 1.1.1.22) 467 468 RXN03056 W0028 6258 6935 PUTATIVE HEXULOSE-6-PHOSPHATE ISOMERASE (EC 469 470 RXN03030 W0009 57006 56443 PERIPLASMIC BETA-GLUCOSIOASEIBETA-XYLOSIDASE PRECURSOR (EC 3.2.1.21) (EC 3.2.1.37) 471 472 RXN00401 W0025 12427 11489 5-DEH-YDR0-4-DEOXYGLUCARATE DEHYDRATASE (EC 4.2.1.41) 473 474 RXN02125 W01 02 23242 22442 ALIDOSE REDUCTASE (EC 1.1.1.21) 475 476 RXN00200 WV0181 1679 5116 arabinosyl transferase subunit B (EC 477 478 RXN01 175 W0017 39688 38303 PHOSPHO-2-DEHYORO-3-DEOXYHEPTONATE ALDOLASE (EC 4.1.2.1 479 480 RXN01376 W0091 5610 4750 PUTATIVE GLYCOSYL TRANSFERASE WBIF 481 482 RXN01631 W0050 47021 46143 PUTATIVE HEXULOSE-6-PHOSPHIATE ISOMERASE (EC 483 484 RXN01 593 W0229 13274 12408 NAGID PROTEIN 485 486 RXN00337 W01 97 20359 21418 GALACTOKINASE (EC 2.7.1.6) C 487 488 RXS00584 W0323 5516 6640 PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE (EC 4.1.2.15) 489 490 RXS02574 BETA-HEXOSAMINIDASE A PRECURSOR (EC 3.2.1.52) 491 492 RXS03215 GLUCOSE-FRUCTOSE OXIDOREDUCTASE PRECURSOR (EC 1.1.99.28) 493 494 F RXA01915 GR00549 1 1008 GLUCOSE-FRUCTOSE OXIDOREDUCTASE PRECURSOR (EC 1.1.99.28) 495 496 RXS03224 CYCLOMALTODEXTRINASE (EC 3.2.1.54) 497 498 F RXA00038 GROO006 1417 260 CYCLOMALTOIDEXTRINASE (EC 3.2.1.54) 499 500 RXC00233 protein involved in sugar metabolism 501 502 RXC00236 Membrane Lipoprotein involved in sugar metabolism 503 504 RXCO0271 Exported Protein involved in ribose metabolism 505 506 RXC00338 protein involved in sugar metabolism 507 508 RXCO0362 Membrane Spanning Protein involved in metabolism of diols 509 510 RXCO0412 Amino Acid ABC Transporter AlP-Binding Protein involved in sugar metabolism 511 512 RXC00526 ABC Transporter ATP-Binding Protein involved in sugar metabolism 513 514 RXCO1004 Membrane Spanning Protein involved in sugar metabolism 515 516 RXCO1017 Cytosolic Protein involved In sugar metabolism 517 518 RXCO1021 Cytosolic Kinase involved in metabolism of sugars and thiamin 519 520 RXCO1212 ABC Transporter ATP-Binding Protein involved in sugar metabolism 521 522 RXCO1 306 Membrane Spanning Protein involved in sugar metabolism 523 524 RXCO1 366 Cytosolic Protein involved in sugar metabolism 525 526 RXC01372 Cytosolic Protein involved in sugar metabolism 2006200985 24 Feb 2006 Table I (continued) Nucleic Acid Amino Acid Identification Code Cti. NT Start INT StoD Function SEQ ID NO SEQ IDNO 527 528 RXCO1659 protein in 529 530 RXCO 1663 protein in 531 532 RXCO 1693 protein in 533 534 RXCO1703 Cytosolic 535 536 RXC02254 Membran 537 538 RXC02255 Cytosolic 539 540 RXC02435 protein in 541 542 F RXA02435 GR00709 825 268 Uncharac valved in sugar metabolism valved in sugar metabolism valved in sugar metabolism Protein involved in sugar metabolism Associated Protein involved in sugar metabolism Protein involved in sugar metabolism valved in sugar metabolism lterized protein involved in glycerol metabolism (homolog of la rhomboid) valved in sugar metabolism Drosophi protein in 544 RXC03216 TCA-cycle Nucleic Acid Amino Acid SEQ ID NO SEQ ID NO 545 546 547 548 549 550 551 552 553 554 555 556 557 558 559 560 561 562 563 564 Identification Code Cni NT Start NT Stop Function RXA02175 RXA02621 RXN00519 F RXA00521 RXN02209 F RXA02209 RXN0221 3 F RXA02213 RXA02056 RXA01 745 RXA00782 RXA00783 RXN01 695 F RXA01615 F RXA01695 RXA00290 RXN01 048 F RXA01048 F RXA00290 RXN03I1 GR00641 10710 GR00746 2647 WV0144 5585 GR00133 2 W0304 1 GR00648 3 WV0305 1378 GR00649 1330 GR00625 3 GR00495 2 GR00206 3984 GR00206 5280 W0139 11307 GR00449 8608 GR00474 4388 GR00046 4693 W0079 12539 GR00296 3 GR00046 4693 WV0066 2 W0025 15056 W0025 11481 9418 CITRATE SYNTHASE (EC 4.1.3.7) 1829 CITRATE LYASE BETA CHAIN (EC 4.1.3.6) 3372 ISOCITRATE DEHYDROGENASE (NADP) (EC 1.1.1.42) 1060 ISOCITRATE DEHYDROGENASE [NADP] (EC 1. 1. 1.42) 1671 ACONITATE HYDRATASE (EC 4.2.1.3) 1661 ACONITATE HYDRATASE (EC 4.2.1.3) 2151 ACONITATE H-YDRATASE (EC 4.2.1.3) 2046 ACONITATE HYDRATASE (EC 4.2.1.3) 2870 2-OXOGLUTARATE DEHYDROGENASE Ell COMPONENT (EC 1.2.4.2) 1495 DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE COMPONENT (E2) OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX (EC 2.3.1.61) 3103 SUCCINYL-COA SYNTHETASE ALPHA CHAIN (EC 6.2.1.5) 4009 SUCCINYL-COA SYNTHETASE BETA CHAIN (EC 6.2.1.5) 12806 1-MALATE DEHYDROGENASE (ACCEPTOR) (EC 1. 1.99.16) 9546 L-MALATE DEHYDROGENASE (ACCEPTOR) (EC 1.1.99.16) 4179 L-MAI.ATE DEHYDROGENASE (ACCEPTOR) (EC 1.1.99.16) 5655 MALIC ENZYME (EC 1. 1. 1.39) 11316 MALIC ENZYME (EC 1. 1.1.39) 290 MALIC ENZYME (EC 1.1.1.39) 5655 MALIC ENZYME (EC 1. 1. 1.39) 583 DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE COMPONENT (E2) OF 2-OXOGLUTARATE DEHYDROGENASE COMPLEX (EC 2.3.1.61) 14640 DIHYDROLIPOAMIDE SUCCINYLTRAINSFERASE COMPONENT OF 2- OXOGLUTARATE DEHYDROGENASE COMPLEX (EC 2.3.1.61) 9922 oxoglutarate semialdehyde dehydrogenase (EC 585 586 RXN02046 587 588 RXND0389 2006200985 24 Feb 2006 Glyoxylate bypass Table I (continued) NT Start NT Stop Function Nucleic Acid SEQ ID NO 589 591 593 595 597 599 Amino Acid Identification Code SEQ ID NO 590 RXN02399 592 F RXA02399 594 RXN02404 596 F RXA02404 598 RXA01 089 600 RXA01886 V01 76 GRO0699 W01 76 GR00700 GR00304 GROO539 19708 478 20259 3798 3209 3203 18365 1773 22475 1663 3958 2430 ISOCITRATE LYASE (EC 4.1.3.1) ISOCITRATE LYASE (EC 4.1.3.1) MALATE SYNTHASE (EC 4.1.3.2) MALATE SYNTHASE (EC 4.1.3.2) GLYOXYLATE-INDUCED PROTEIN GLYOXYLATE-INDUCED PROTEIN Methylcitrate-pathway Nucleic Acid SEQ ID NO 600 601 603 605 607 609 611 613 615 617 619 621 623 Amino Acid Identification Code SEQ ID NO 602 RXN03117 604 F RXA00406 606 F RXA00514 608 RXA00512 610 RXA00518 612 RXA01077 614 RXN03144 616 F RXA02322 618 RXA02329 620 RXA02332 622 RXN02333 624 F RXA02333 626 RXAC0030 Cionti W0092 GROO090 GROO130 GROO1 30 GROO1 31 GROO300 W0141 GRO0668 GR00669 GR00671 W0141 GR00671 GROO003 NT Start NT Stop 3087 978 1983 621 3069 4647 2 415 607 1906 901 2120 9590 1576 4 1576 4 2773 6017 901 5 5 764 1815 1902 9979 Function 2-methylisocitrate synthase (EC 2-methylisocitrate synthase (EC 2-methylisocitrate synthase (EC 2-methylcitrate synthase (EC 4.1 .3.31) 2-methylcitrate synthase (EC 4.1.3.31) 2-methylisocitrate synthase (EC 2-methylisocitrate synthase (EC 2-methylisacitrate synthase (EC 2-methylisocitrate synthase (EC 2-methylcitrate synthase (EC 4.1.3.31) methylisocitrate lyase (EC 4.1.3.30) methylisocitrate lyase (EC 4.1.3.30) LACTOYLGLUTATHIONE LYASE (EC 4.4.1.5) Methyl-Malonyl-CoA-Mutases Nucleic Acid Amino Acid Identification Code SEQ ID NO SEQ IDNO 625 628 RXN00148 627 630 F RXA00148 629 632 RXA00149 Contig.

W0167 GR00023 GROO023 NT Start NT Stop Function 9849 2002 3856 12059 5 2009 METHYLMALONYL-COA MUTASE ALPHA-SUBUNIT (EC 5.4.99.2) METHYLMALONVL-COA MUTASE ALPHA-SUBUNIT (EC 5.4.99.2) METHYLMALONYL-OA MUTASE BETA-SUBUNIT (EC 5.4.99.2) 2006200985 24 Feb 2006 Table 1 (continued) Others Nucleic Acid Amino Acid Identification Code Config. NT Start NT Stop Function SEQ ID NO SEQ ID NO 631 634 RXN00317 WV0197 26879 27532 PHOSPHOGLYCOLATE PHOSPHATASE (EC 3.1.3.18) 635 636 F RXA00317 GROO055 344 6 PHOSPHOGLYCOLATE PHOSP-ATASE (EC 3.1.3.18) 637 638 RXA02196 GRO0645 3956 3264 PHOSPHOGLYCOLATE PHOSPHATASE (EC 3.1.3.18) 639 640 RXN02461 W0124 14236 14643 PHOSPHOGLYCOLATE PH-OSPHATASE (EC 3.1.3.18) Redox Chain Nucleic Acid Amino Acid Identification Code oni. NT Start NT Stop Function SEQ ID NO SEQ ID NO 641 642 R.XN01744 WV0174 2350 812 CYTOONROME D UBIQUINOL OXIDASE SUBUNIT I (EC 1.10.3.-) 643 644 F RXA00055 GROO008 11753 11690 CYTOCHROME D UBIQUINOL OXIDASE SUBUNIT I (EC 1.10.3.-) 645 646 F RXA01744 GR00494 2113 812 CYTOCH-ROME D UBiQUINOL OXIDASE SUBUNIT I (EC 1.10.3.-) 647 648 RXA00379 GR00082 212 6 CYIOCHROME C-TYPE BIOGENESIS PROTEIN CCDA 649 650 RXA00385 GR00083 773 435 CYTOCHROME C-TYPE BIOGENESIS PROTEIN CCDA 651 652 RXA01743 GR00494 806 6 CYTOCHROME 0 UBIQUINOL OXIDASE SUBUNIT 11 (EC

C

653 654 RXN02480 WV0084 31222 29567 CYTOCHROME C OXIDASE POLYPEPTIDE I (EC 1.9.3.1) 655 656 F RXA01 919 GROO550 288 4 CYTOCHROME C OXIDASE SUBUNIT I (EC 1.9.3.1) 657 658 F RXA02480 GR00717 1449 601 CYTOCHROME C OXIDASE POLYPEPTIDE I (EC 1.9.3.1) 659 660 F RXA02481 GR00717 1945 1334 CYTOCHROME C OXIDASE POLYPEPTIDE I (EC 1.9.3.1) 661 662 RXA02140 GR00639 7339 8415 CYTOCHROME C OXIDASE POLYPEPTIDE 11 (EC 1.9.3.1) 663 664 RXA02142 GR00639 9413 10063 CYTOCHROME C OXIDASE POLYPEPTIDE I (EC 1.9.3.1) 665 656 RXA02144 GR00639 11025 12248 RIESKE IRON-SULFUR PROTEIN 667 668 RXA02740 GR00763 7613 8542 PROBABLE CYTOCHROME C OXIDASE ASSEMBLY FACTOR 669 670 RXA02743 GR00763 13534 12497 CYTOCHROME AA3 CONTROLLING PROTEIN 671 672 RXA01227 GR00355 1199 1519 FERREDOXIN 673 674 RXA01865 GR00532 436 122 FERREDOXIN 675 676 RXA00680 GR00179 2632 2315 FERREDOXIN VI 677 678 RXA00679 GR00179 2302 1037 FERREDOXIN-NAD(+) REDUCTASE (EC 1.18.1.3) 679 680 RXA00224 GR00032 24965 24015 ELECTRON TRANSFER FLAVOPROTEIN ALPHA-SUBUNIT 681 682 RXA00225 GR00032 25783 24998 ELECTRON TRANSFER FLAVOPROTEIN BETA-SUBUNIT 683 684 RXN00606 WV0192 11299 9026 NADHODEHYDROGENASE I CHAIN L (EC 1.6.5.3) 685 686 F RXA00606 GROO160 121 1869 NADH DEHYDROGENASE I CHAIN L (EC 1.6.5.3) 687 688 RXN00595 WV01 92 8642 7113 NADHODEHYDROGENASE I CHAIN M (EC 1.6.5.3) 689 690 F RXA00608 GROO160 2253 3017 NAOH DEHYDROGENASE I CHAIN M (EC 1.6.5.3) 691 692 RXA00913 GR00249 3 2120 NADH DEHYDROGENASE I CHAIN L (EC 1.6.5.3) 693 694 RXA00909 GR00247 2552 3406 NADH DEHYDROGENASE I CHAIN L (EC 1.6.5.3) 695 696 RXA00700 GROO1 82 846 43 NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 2 697 698 RXN00483 WV0086 44824 46287 NADH-UBIQUINONE OXIDOREDUCTASE 39 KD SUBUNIT PRECURSOR (EC 1.6.5.3) (EC 1.6.99.3) 2006200985 24 Feb 2006 Nucleic Acid SEQ ID NO 699 Table 1 (continued) NT Start NT Stop Function Amino Acid SEQ ID NO 700 Identification Code F RXA00483 RXA01 534 RXA00288 RXA02741 RXN02560 F RXA02560 RXA01 311 RXN03014 F RXA0091 0 RXN01 895 F RXA01895 RXA00703 RXN00705 F RXA00705 RXN00388 F RXA00388 F RXA00386 RXA00945 RXN02556 F RXA02556 RXA01 392 RXA00800 RXA021 43 RXN03096 RXN02036 RXN02765 RXN02206 RXN02554 Contig GRO01 19 GROD427 GR00046 GR00763 wolol1 GR00731 GR00380 VV0058 GR00248 WV01 17 GR00543 GROO183 W0005 GRO0184 W0025 GROO085 GROO084 GR00259 V0 101 GR00731 GR00408 GROD214 GR00639 WV0058 WV0176 W0317 W0302 wolol1 19106 1035 2646 9585 9922 6339 1611 1273 3 955 2 2556 6111 1291 2081 969 514 1876 5602 2019 2297 2031 10138 405 32683 3552 1784 4633 20569 547 1636 8620 10788 7160 865 368 1259 5 817 271 5197 407 3091 667 5 2847 6759 3176 3373 3134 11025 4 33063 2794 849 4010 NADH-UJBIQUINONE OXIDOREDUCTASE 39 KD SUBUNIT PRECURSOR (EC 1.6.5-3) (EC 1.6.99.3) NADH-DEPENDENT FMVN OXYDOREDUCTASE QUINONE OXIDOREDUCTASE (EC 1.6.5.5) QUINONE OXIDOREDUCTASE (EC 1.6.5.5) NADPH-FLAVIN OXIDOREDUCTASE (EC 1.6.99.-) NADPH-FLAVIN OXIDOREDUCTASE (EC 1.6.9g.-) SUCCINATE DEHYDROGENASE IRON-SULFUR PROTEIN (EC 1.3.99.1) NADH DEHYDROGENASE I CHAIN M (EC 1.6.5.3) H-ydrogenase subunits NADH DEHYDROGENASE (EC 1.6.99.3)

DEHYDROGENASE

FORMATE DEHYDROGENASE ALPHA CHAIN (EC 1.2.1.2) FDHD PROTEIN FOHO PROTEIN CYTOCHROME C BIOGENESIS PROTEIN CCSA essential protein similar to cytochrome c RESC PROTEIN, essential protein similar to cytochrome c biogenesis protein putative cytochrome oxidase FLAVOHEMOPROTEIN DIHYDROPTERIDINE REDUCTASE (EC 1.6.9.7)

FLAVOHEMOPROTEIN

GLUTATHIONE S-TRANSFERASE (EC 2.5.1.18) GLUTATH-IONE-DEPENDENT FORMALDEHYDE DEHYDROGENASE (EC 1.2.1.1) QCRC PROTEIN, men aquinol:cytochrome c oxidoreductase NADH DEHYDROGENASE I CHAIN M (EC 1.6.5.3) NADH-UBIQUINONE OXIDOREDUCTASE CHAIN 4 (EC 1.6.5.3) Hypothetical Oxidorductase Hypothetical Oxidoreductase Hypothetical Oxidoreductase (EC 1. 1. 733 734 735 736 ATP-Synthase Nucleic Acid SEQ ID NO 755 757 759 761 763 765 Amino Acid SEQ ID NO 756 758 760 762 764 766 Identification Code RXN01 204 F RXA01 204 RXA01 201 RXN01193 F RXA01 193 F RXA01203 WV0121 GR00345 GR00344 W0175 GR00343 GROO344 NT Start NT Stop Function 1270 394 675 5280 15 3355 ATP SYNTHASE A CHAIN (EC 3.6.1.34) ATP SYNTHASE A CHAIN (EC 3.6.1.34) ATP SYNTHASE ALPHA CHAIN (EC 3.6.1.34) ATP SYNTHASE BETA CHAIN (EC 3.6.1.34) ATP SYNTHASE BETA CHAIN (EC 3.6.1.34) ATP SYNTHASE BETA CHAIN (EC 3.6.1.34) 2006200985 24 Feb 2006 Table I (continued) NT Start NT Stop Funcion Nucleic Acid SEQ ID NO 767 769 771 773 775 777 Amino Acid Identification Code Cni.

SEQ ID NO 768 RXN02821 WV0121 770 F RXA02821 GR00802 772 RXA01 200 GR00344 774 RXA01 194 GROO343 776 RXA01 202 GR00344 778 RXN02434 W0090 324 1139 2 770 2375 4923 85 318 610 1141 3349 3274 ATP SYNTHASE C CHAIN (EC 3.6.1.34) ATP SYNTHASE C CHAIN (EC 3.6.1.34) ATP SYNTHASE DELTA CHAIN (EC 3.6.1.34) ATP SYNTH-ASE EPSILON CHAIN (EC 3.6.1.34) ATP SYNTHASE GAMMA CHAIN (EC 3.6.1.34) ATP-BINDING PROTEIN Cytochrome metabolism Nucleic Acid SEQ ID NO 779 781 Amino Acid Identification Code Cnu.

SEQ ID NO 780 RXN00684 WO000S 782 RXN00387 VV0025 NT Start NT Stop Function 29864 285811 CYTOCHROME P450 116 (EC 1150 2004 Hypothetical Cytochrome c Biogenesis Protein 2006200985 24 Feb 2006 2 Excluded Genes GenBank'" Gene Name Gene Function Reference Accession No.

A09073 ppg Phosphoenol pyruvate carboxylase Bachmann, B. et al. "DNA fragment coding for phosphoenolpyruvat corboxylase, recombinant DNA carrying said fragment, strains carrying the recombinant DNA and method for producing L-aminino acids using said strains," Patent: EP 0358940-A 3 03/21/90 A45579, Threonine dehydratase Moeckel, B. et al. "Production of L-isoleucine by means of recombinant A45581, micro-organisms with deregulated threonine dehydratase," Patent: WO A45583, 95 19442-A 5 07/20/95 A45585 A45587 AB003 132 murC; ftsQ; fisZ Kobayashi, M. et at. "Cloning, sequencing, and characterization of the ftsZ gene from coryneform bacteria," Biochem. Biophys. Res. Comm un., (1997) ABC 15023 murC; ftsQ Wachi, M. et al. "A murC gene from Coryneform bacteria," Appi. Microbiol 51 (2):223-228 (1999) ABC 18530 dtsR Kimura, E. et al. "Molecular cloning of a novel gene, dtsR, which rescues the detergent sensitivity of a mutant derived from Brevibacterium ____________lactofermentum," Biosci. BiotechnoL Biochem., 60(10):1565-1570 (1996) AB018531 dtsRl; dtsR2 AB020624 murl D-glutamate racemase AB023377 tkt transketolase AB024708 gltB; gltD Glutamine 2-oxoglutarate aminotransferase large and small subunits AB025424 acn aconitase AB027714 rep Replication protein AB027715 rep; aad Replication protein; aminoglycoside _____________adenyltransferase AF005242 argC N-acetylglutamate-5-sem ialdehyde Sdehydrogenase AF005635 gInA Glutamine synthetase AF030405 hisF cyclase AF030520 argG Argininosuccinate synthetase AF031518 argF Omnithine carbamolytransferase AF036932 aroD) 3-dehydroquinate dehydratase AF038548 PYC Pyruvate carboxylase 2006200985 24 Feb 2006 Table 2 (continued) AF038651I dciAE; apt; rel Dipeptide-binding protein; adenine Wehmeier, L. et al. "The role of the Corynebacterium glutamicum rel gene in phasphoribosyltransferase; GTP (p)ppGpp metabolism," Microbiology 144:1853-1862 (1998) AF04 1436 argR Arginine AF045998 impA Inositol monophosphate AF048764 argH Argininosuccinate AF049897 argC; argi; argB; N-acety lg Iutamyl phosphate reductase; argD; argF; argR; omnithine acetyltransferase; NargG; argH acetylgiutamnate kinase; acetylornithine transminase; omnithine carbamoyltransferase; arginine repressor; argininosuccinate synthase; argininosuccinate AF050 109 inbA Enoyl-acyl carrier protein reductase AF050 166 hisG ATP phosphoribosyltransferase 1846 hisA Phosphoribosylform imino-5-amino- Iphosphoribosyl-4- imidazolecarboxamide isomerase AF052652 metA Homoserine 0-acetyltransferase Park, S. et al. "Isolation and analysis of metA, a methionine biosynthetic gene encoding homoserine acetyltransferase in Corynebacteriumn glutamicum," Mol Cells., 8(3):286-294 (1998) AF053071 aroB Dehydroquinate AF060558 hisH Glutamine am idotransferase AF086704 hisE Phosphoribosyl-ATPpyrophosphohydrolase AF 14233 aroA 5-enolpyruvylshikimate 3-phosphate synthase AFR 16184 panD L-aspartate-alpha-decarboxylase precursor Dusch, N. et al. "Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-dlecarboxy lase leads to pantothenate overproduction in Escherichia, coli," Appi. Environ. Microbiol., 65(4)1530- (1999) AF124518 aroD; aroE 3-dehydroquinase; shikimate AF124600 aroC; aroK; aroB; Chorismate synthase; shikimate kinase; 3pepQ dehydroquinate synthase; putative AF145897 AF145898 inhA 2006200985 24 Feb 2006 Table 2 (continued) AJO0 1436 ectP Transport of ectoine, glycine betaine, Peter, H. et "Corynebacterium glutamicumn is equipped with four secondary proline carriers for compatible solutes: Identification, sequencing, and characterization of the proline/ectoine uptake system, ProP, and the ectoine/proline/glycine betaine carrier, EctP," J Bacterial, 1 80(22):6005-60 12 (1998) AJ004934 dapD Tetrahydrodipicolinate succinylase Wehrmann, A. et al. "Different modes of diaminopimelate synthesis and their (incomplete) role in cell wall integrity: A study with Corynebacterium glutamicum," J 180(12):3159-3165 (1998) AJ007732 ppc; secG; amt; ocd; Phosphoenolpyruvate-carboxylase; high soxA affinity ammonium uptake protein; putative ornithine-cyclodecarboxylase; sarcosine oxidase AJO103 19 ftsY, glnB, gInD; srp; Involved in cell division; PI I protein; Jakoby, M. et al. "Nitrogen regulation in Corynebacteriumn glutamicum; amntP uridylyltransferase (uridylyl-removing Isolation of genes involved in biochemical characterization of corresponding enzniye); signal recognition particle; low proteins," FEMS Microbiol., 173(2):303-310 (1999) ammonium uptake protein AJ 132968 cat Chloramphenicol aceteyl transferase A1224946 mqo L-malate: quinone oxidoreductase Molenaar, D. et al. "Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium glutamicum," Eur. J. Biachem., 254(2):395-403 (1998) AJ238250 ndh NADH dehydrogenase AJ238703 porA Porin Lichtinger, T. et al. "Biochemical and biophysical characterization of the cell wall porin of Corynebacteriumn glutamicum: The channel is formed by a low mass polypeptide," Biochemistry, 37(43):15024-15032 (1998) D17429 Transposable element IS31831 Vertes et al."Isolation and characterization ofIS3183l1, a transposable element Corynebacterium glutamicum," Mat. MicrobioL, 1 1(4):739-746 (1994) D84 102 odhA 2-oxoglutarate dehydrogenase Usuda, Y. et al. "Molecular cloning of the Corynebacteriumn glutamicumn (Brevibacterium lactofermentum Al 12036) odhA gene encoding a novel type 2-oxoglutarate dehydrogenase," Microbiology, 142:3347-3354 (1996) E01358 hdh; hk Homoserine dehydrogenase; homoserine Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent: JP_ 1987232392-A 1 10/12/87 E01359 -Up-stream of the start codon of homoserine Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent: JP kinase gene 1987232392-A 2 10/12/87 E0 1375 Tryptophan operon E01376 trpL; trpE Leader peptide; anthranilate synthase Matsui, K. et al. "Tryptophan operon, peptide and protein coded thereby, utilization of tryptophan operon gene expression and production of Patent: JP 1987244382-A 1 10/24/87 2006200985 24 Feb 2006 Table 2 (continued) E01377 Promoter and operator regions of Matsui, K. et al. "Tryptophan operon, peptide and protein coded thereby, tryptophan operon utilization of tryptophan operon gene expression and production of tryptophan," Patent: JP 1987244382-A 1 10/24/87 E03937 Biotin-synthase Hatakeyama, K. et al. "DNA fragment containing gene capable of coding biotin synthetase and its utilization," Patent: JP 1992278088-A 1 10/02/92 E04040 Diamino pelargonic acid aminotransferase Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A 1 11/18/92 E04041 Desthiobiotinsynthetase Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A 1 11/18/92 E04307 Flavum aspartase Kurusu, Y. et al. "Gene DNA coding aspartase and utilization thereof," Patent: JP 1993030977-A 1 02/09/93 E04376 Isocitric acid lyase Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 1993056782-A 3 03/09/93 E04377 Isocitric acid lyase N-terminal fragment Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 1993056782-A 3 03/09/93 E04484 Prephenate dehydratase Sotouchi, N. et al. "Production of L-phenylalanine by fermentation," Patent: JP 1993076352-A 2 03/30/93 E05108 Aspartokinase Fugono, N. et al. "Gene DNA coding Aspartokinase and its use," Patent: JP 1993184366-A 1 07/27/93 E05112 Dihydro-dipichorinate synthetase Hatakeyama, K. et al. "Gene DNA coding dihydrodipicolinic acid synthetase and its use," Patent: JP 1993184371-A 1 07/27/93 E05776 Diaminopimelic acid dehydrogenase Kobayashi, M. et al. "Gene DNA coding Diaminopimelic acid dehydrogenase and its use," Patent: JP 1993284970-A 1 11/02/93 E05779 Threonine synthase Kohama, K. et al. "Gene DNA coding threonine synthase and its use," Patent: JP 1993284972-A 1 11/02/93 E06110 Prephenate dehydratase Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," Patent: JP 1993344881-A 1 12/27/93 E061 11 Mutated Prephenate dehydratase Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," Patent: JP 1993344881-A 1 12/27/93 E06146 Acetohydroxy acid synthetase Inui, M. et al. "Gene capable of coding Acetohydroxy acid synthetase and its use," Patent: JP 1993344893-A 1 12/27/93 E06825 Aspartokinase Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A I 03/08/94 E06826 Mutated aspartokinase alpha subunit Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A I 03/08/94 2006200985 24 Feb 2006 Table 2 (continued) E06827 Mutated aspartokinase alpha subunit Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A 1 E07701 secY Honno, N. et al. "Gene DNA participating in integration of membraneous protein to membrane," Patent: JP 1994169780-A 1 06/21/94 E08 177 Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase released from inhibition and its utilization," Patent: JP 199426 1766-A 1 09/20/94 E081378, Feedback inh ib ition- released Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase released from E08 179, feedback inhibition and its utilization," Patent: JP 1994261766-A 1 09/20/94 E08 180, E08181 E08 182 E08232 Acetohydroxy-acid isomeroreductase Inui, M. et al. "Gene DNA coding acetohydroxy acid isomeroreductase," JP 1994277067-A I 10/04/94 E08234 secE Asai, Y. et al. "Gene DNA coding for translocation machinery of protein," JP 1994277073-A 1 10/04/94 E0863 FT aminotransferase and desthiobiotin Hatakeyama, K. et al. "DNA fragment having promoter function in synthetase promoter region coryneform bacterium," Patent: JP 199503 1476-A 1 02/03/95 E08646 Biotin synthetase Hatakeyama, K. et al. "DNA fragment having promoter function in bacterium," Patent: JP 1995031476-A I 02/03195 E08649 Aspartase Kohama, K. et al "DNA fragment having promoter function in coryneformn Patent: JP 1995031478-A 1 02/03/95 E08900 Dihydrodipicolinate reductase Madori, M. et al. "DNA fragment containing gene coding Dihydrodipicolinate reductase and utilization thereof," Patent: JP 1995075578-A 1 03/20/95 £08901 Diaminopimnelic acid decarboxylase Madori, M. et al. "DNA fragment containing gene coding Diaminopimelic acid ____________decarboxylase and utilization thereof," Patent: JP 1995075579-A I103/20/95 E12594 Serine hydroxymethyltransferase Hatakeyama, K. et al. "Production of L-trypophan," Patent: JP 1997028391-A 02/04/97 E£12760, transposase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: E12759, JP 1997070291-A 03/18/97 £12764 Arginyl-tRNA synthetase; diaminopimnelic Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: acid decarboxylase J P 1997070291]-A 03/18/97 E12767 Dihydrodipicolinic acid synthetase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: 199707029 1-A 03/18/97 E12770 aspartokinase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291]-A 03/18/97 £12773 Dihydrodipicolinic acid reductase Moriya, M. et al. "Amplification of gene using artificial transpason," Patent: 1997070291 -A 03/18/97 2006200985 24 Feb 2006 2 (continued) E13655 G lucose-6- phosphate dehydrogenase Hatakeyama, K. et al. "Glucose-6-phosphate dehydrogenase and DNA capable coding the same," Patent: JP 199722466 1 -A 1 09/02/97 LO 1508 INvA Threonine dehydratase Moeckel, B. et al. "Functional and structural analysis of the threonine dehydratase of Corynebacterium glutamicum," J Bacteriol, 174:8065-8072 (1992) L07603 EC 4.2.1.15 3-deoxy-D-arabinoheptulosonate-7- Chen, C. et al. "The cloning and nucleotide sequence of Corynebacterium phosphate synthase glutamicum 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase gene," Microbial Left., 107:223-230 (1993) L09232 llvB; ilvN; ilvC Acetohydroxy acid synthase large subunit; Keilhauer, C. et al. "Isoleucine synthesis in Corynebacterium glutamicum: Acetohydroxy acid synthase small subunit; molecular analysis of the ilvB-ilvN-ilvC operon," J Bacterial., 175(17):5595acid isomeroreductase 5603 (1993) L18874 PtsM Phosphoeniolpyruvate sugar Fouet, A et al. "Bacillus subtilis sucrose-specific enzyme 11 of the phosphotransferase phosphotransferase system: expression in Escherichia coli and homology to enzymes 11 from enteric bacteria," PNAS USA, 84(24):8773-8777 (1987); Lee, J.K. et al. "Nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme 11 and analyses of the deduced protein FEM4S Microbial Lett., 1 19(1-2):137-145 (1994) L27 123 aceB Malate synthase Lee, H-S. et al. "Molecular characterization of aceB, a gene encoding malate synthase in Corynebacterium glutam icum," J Microbial. BiotechnoL, 4(4):256-263 (1994) L27 126 Pyruvate kinase Jetten, M. S. et al. "Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum," AppI. Environ. Microbial, 60(7):2501-2507 (1994) L28760 aceA Isocitrate lyase L35906 dtxr Diphtheria toxin repressor Oguiza, J.A. et al. "Molecular cloning, DNA sequence analysis, and characterization of the Corynebacterium diphtheriae dtxR from Brevibacterium lactofermentum," J Bacterial.. 1 77(2):465-467 (1995) M13774 Prephenate dehydratase Follettie, M.T. et al. "Molecular cloning and nucleotide sequence of the Corynebacterium glutamicum pheA gene," J Bacterial, 167:695-702 (1986) M16175 5S rRNA Park, Y-H. et al. "Phylogenetic analysis of the coryneformn bacteria by 56 sequences," J Bacterial, 169:1801-1806 (1987) M 16663 trpE Anthranilate synthase, 5' end S9ano, K. et al. "Structure and function of the trp operon control regions of Brevibacterium lactofermentum, a glutamic-acid-producing bacterium," Gene, (1987) *M16664 trpA Tryptophan synthase, 3'end Sano, K. et al. "Structure and function of the trp operon control regions of Brevibacterium lactoferinentum, a glutamic-acid-producing bacterium," Gene, (1987) 2006200985 24 Feb 2006 Table 2 (continued) M25919 Phosphoenolpyruvate carboxylase O'Regan, M. et al. "Cloning and nucleotidle sequence of the Phosphoenolpyruvate carboxylase-coding gene of Corynebacteriumn glutamicum ATCC 13032," Gene, 77(2):237-25 1 (1989) 106 23S rRNA gene insertion sequence Roller, C. et al. "Gram-positive bacteria with a high DNA G+C content are characterized by a common insertion within their 23S rRNA genes," J Gen.

Microbiol, 138:1167-1175 (1992) 107, 23S rRNA gene insertion sequence Roller, C. et al. "Gram-positive bacteria with a high DNA G+C content are 108 characterized by a common insertion within their 23S rRNA genes," J. Gen.

138:1167-1 175 (1992) M89931 aecD; bmQ; yhbw Beta C-S lyase; branched-chain amino acid Rossol, 1. et al. "The Corynebacterium glutamicum aecD gene encodes a C-S uptake carrier; hypothetical protein yhbw lyase with alpha, beta-elimination activity that degrades aminoethylcysteine," J. Baceriol, 174(9):2968-2977 (1992); Tauch, A. et al. "Isoleucine uptake in Corynebacteriumn glutamicumn ATCC 13032 is directed by the brnQ gene Arch. MicrobioL, 1 69(4):303-3 12 (1998) S59299 trp Leader gene (promoter) Herry, D.M. et al. "Cloning of the trp gene cluster from a tryptophanhyperproducing strain of Corynebacterium glutamicum: identification of a mutation in the trp leader sequence," App. Environ. Microbiol, 59(3):791-799 Ut 11545 trpD Anthranilate phosphoribosyltransferase O'Gara, J.P. and Dunican, L.K. (1994) Complete nucleotidle sequence of the Corynebacteriumn glutamicum ATCC 21850 tpD gene." Thesis, Microbiology University College Galway, Ireland.

U 13 922 cgllM; cglIR; cIglIR Putative type 11 5-cytosoine Schafer, A. et al. "Cloning and characterization of a DNA region encoding a methyltransferase; putative type 11 stress-sensitive restriction system from Corynebacteriumn glutamicum ATCC restriction endlonuclease; putative type I or 13032 and analysis of its role in intergeneric conjugation with Eseherichia type III restriction endlonuclease coli," J Bacteril., 176(23):7309-7319 (1994); Schafer, A. et al. "The Corynebacteriumn glutamicum cgllM gene encoding a 5-cytosine in an McrBCdeficient Escherichia coli strain," Gene, 203(2):95-101 (1997) U 14965 U31224 PPX Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline biosynthetic pathway: A natural bypass of the proA step," J BacterioL, 178(15):44 12-44 19 (1996) U31225 proC L-proline: NADP+ 5-oxidloreductase Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline biosynthetic pathway: A natural bypass of the proA step," J BacteriaL, (1996) U3 1230 obg, proB3; unkdh ?;gamma glutamyl kinase;similar to D- Ankri, S. et al. "Mutations in the Corynebacterium glutamnicumproline isomer specific 2-hydroxyacid biosynthetic pathway: A natural bypass of the proA step," J Bacterial., ______________dehydrogenases 178(1 5):44 12-4419 (1996) 2006200985 24 Feb 2006 Table 2 (continued) U31281 bloB Biotin synthase Serebriiskii, "Two new members of the bio B superfamily: Cloning, sequencing and expression of bio B genes of Methylobacillus flagellatumn and ____________Corynebacterium glutamicum," Gene, 175:15-22 (1996) U35023 thtR; accBC Thiosulfate sulfurtransferase; acyl CoA Jager, W. et al. "A Corynebacterium glutamicum gene encoding a two-domain carboxylase protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins," Arch. Microbial., 166(2);76-82 (1996) U43535 cmr Multidrug resistance protein Jager, W. et al. "A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli," J Bacterial., 1 79(7):2449-2451I (1997) U43536 clpB Heat shock ATP-binding protein U53587 aphA-3 3 '5 '-aminoglycoside phosphotransferase U89648 Corynebacterium glutamicum unidentified sequence involved in histidine biosynthesis, partial sequence X04960 trpA; trpB; trpC; trpD; Tryptophan operon Matsui, K. et al. "Complete nucleotide and deduced amino acid sequences of trpE; trpG; trpL the Brevibacterium lactofermentum tryptophan operon," Nucleic Acids Res., (1986) X07563 lys A DAP decarboxylase (meso-diam inopime late Yeh, P. et al. "Nucleic sequence of the lysA gene of Corynebacterium, decarboxylase, EC 4.1.1.20) glutamicum and possible mechanisms for modulation of its expression," Mol.

Genet., 212(1):112-119 (1988) X14234 EC 4.1.1.31 Phosphoenolpyruvate carboxylase Eikmanns, BiJ. et al. "The Phosphoenolpyruvate carboxylase gene of Corynebacterium glutamnicum: Molecular cloning, nucleotide sequence, and expression," Mo!. Gen. Genet., 218(2):330-339 (1989); Lepiniec, L. et al.

"Sorghum Phosphoenolpyruvate carboxylase gene family: structure, function and molecular evolution," Plant. Mlo!. Biol., 21 (3):487-502 (1993) X 17313 fda Fructose-bisphosphate aldolase Von der Osten, C.H. et al. "Molecular cloning, nucleQtide sequence and finestructural analysis of the Corynebacteriumn glutamicum fda gene: structural comparison of C. glutamicumn fiructose-I1, 6-biphosphate aldolase to class I and class 11 aldolases," Mo!. Microbial., X53993 dapA L-2, 3-dihydrodipicolinate synthetase (EC Bonnassie, S. et al. "Nucleic sequence of the dapA gene from 4.2.1.52) Corynebacterium glutamicum," Nucleic Acids Res., 18(21).6421 (1990) X54223 AttB-related site Cianciotto, N. et al. "DNA sequence homology between att B3-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum, and the attP site of lambdacorynephage," FEMS. Microbiol, Let.,66:99-02(1990) X54740 argS; lysA Arginyl-tRNA synthetase; Diaminopimelate Marcel, T. et al. "Nucleotide sequence and organization of the upstream region decarboxylase of the Corynebacterium glutamicum lysA gene," Mo!. Microbial., 4(1 1):18 19- (1990) 2006200985 24 Feb 2006 2 (continued) X55994 trpL; trpE Putative leader peptide; anthranilate Heery, D.M. et al. "Nucleotide sequence of the Corynebacteriumn glutamicum component I trpE gene," Nucleic Acids Res.. 18(23):7138 (1990) X56037 thrC Threonine synthase Han, K.S. eta!. "The molecular structure of the Corynebacteriumn glutamicum synthase gene," Mat. Microbial., 4(10):1693-1702 (1990) X56075 attB-related site Attachment site Cianciotto, N. et al. "DNA sequence homology between att B-related sites of- Corynebacteriumn diphtheriae, Corynebacteriumn ulcerans, Corynebacterium glutamicum and the attP site of lambdacorynephage," FEMS. Microbial, 66:299-302 (1990) X57226 lysC-alpha; lysC-beta; Aspartokinase-aipha subunit; Kalinowski, J. et al. "Genetic and biochemical analysis of the Aspartokinase asd Aspartokinase-bela subunit; aspartate beta from Corynebacterium glutamicum," Mol. MicrobiaL, 5(5):1 197-1204 (1991); semialdehyde dehydrogenase Kalinowski, J. et al. "Aspartokinase genes lysC alpha and lysC beta overlap and are adjacent to the aspertate beta-semialdehyde dehydrogenase gene asd in ___________Corynebacteriumn glutamicum," Mat. Gen. Gene., 224(3):317-324 (1990) X59403 gap;pgk; tpi G lycera ldehyde- 3-phosphate; Eikmanns, BiJ. "Identification, sequence analysis, and expression of a phosphoglycerate kinase; triosephosphate Corynebacterium glutamicum gene cluster encoding the three glycolytic isomerase enzymes glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomeras," J. BacteriaL., 174(19):6076-6086 X59404 gdh Glutamate dehydrogenase Bormann, E.R. et "Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase," MoL MicrobiaL, 6(3):3 17-326 X60312 lysi L-lysine permease Seep-Feidhaus, A.H. et al. "Molecular analysis of the Corynebacteriumn glutamicum lysI gene involved in lysine uptake," Mat. M~icrobial., 5(12):2995- (1991) X66078 cop I Psi protein Joliff, G. et al. "Cloning and nucleotidle sequence of the csplI gene encoding PSI, one of the two major secreted proteins of Corynebacterium glutamicum: The deduced N-terminal region of PSI is similar to the Mycobacterium antigen X66 12 lt itrte yntase85 complex," M16. Micrabial., 6(16):2349-2362 (1992) X6612 gi CiratesyntaseEikmanns, B.J. et al. "Cloning sequence, expression and transcriptional analysis of the Corynebacterium glutamicum gItA gene encoding citrate Microbial., 140:1817-1828 (1994) X67737 dapB Dihydrodipicolinate reductase X69 103 csp2 Surface layer protein PS2 Peyret, i.L. et "Characterization of the cspB gene encoding PS2, an ordered surface-layer protein in Corynebacterium glutamicum," At6. Microbial., re la te d _in s e rtio n_ __ele m e n t_ 9 1 :9 7 1 0 9 1 9 9 3 X69 104 S3rltdisrinemntBonamy, C. et al. "Identification of IS 1206, a Corynebacterium glutamicum 1S3-related insertion sequence and phylogenetic analysis," Mat. Microbial., (1994) 2006200985 24 Feb 2006 2 (continued) X70959 IeuA Isopropylmalate synthase Patek, M. et al. "Leucine synthesis in Corynebacterium glutamicum: enzyme activities, structure of IeuA, and effect of leuA inactivation on lysine synthesis," AppI. Environ. Microbial., 60(1):133-140 (1994) X71489 icd Isocitrate dehydrogenase (NADP+) Eikmanns, B.J. et al. "Cloning sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme," J Bacterial, 177(3):774-782 (1995) X72855 GDHA Glutamate dehydrogenase (NADP+) X75083, mtrA 5-methyltryptophan resistance Heery, D.M. et al. "A sequence from a tryptophan-hyperproducing strain of X70584 Corynebacterium glutamicum encoding resistance to Biophys. Res. Commun., 201(3): 1255-1262 (1994) X75085 recA Fitzpatrick, R. et al. "Construction and characterization of recA mutant strains of Corynebacterium glutamicum and Brevibacterium lactofermentum," Appi.

Biolechnol, 42(4):575-580 (1994) X75504 aceA; thiX Partial Isocitrate lyase; Reinscheid, D.J. et al. "Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme," J 176(1 2):3474-3483 (1994) X76875 ATPase beta-subunit Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit Antonie Van Leeuwenhoek, 64:285-305 (1993) X77034 tuf Elongation factor Tu Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit Antonie Van Leeuwenhoek 64:285-305 (1993) X77384 recA Billman-Jacobe, H. "Nucleotide sequence of a recA gene from glutamicum," DNA Seq., 4(6):403-404 (1994) X78491 aceB Malate synthase Reinscheid, DiJ. et al. "Malate synthase from Corynebacterium glutamicum pta-ack operon encoding phosphotransacetylase: sequence analysis," 140:3099-3 108 (1994) X80629 16S rDNA 16S ribosomal RNA Rainey, F.A. et al. "Phylogenetic analysis of the genera Rhodococcus and Norcardia and evidence for the evolutionary origin of the genus Norcardia from within the radiation of Rhodococcus species," MicrobioL, 141:523-528 (1995) X81 191 gluA; gluB; gluC; Glutamate uptake system Kronemeyer, W. et al. "Structure of the gluABCD cluster encoding the gluD glutamate uptake system of Corynebacterium glutamicum," J Bacterial, 152-1 158 (1995) X81379 dapE Succinyldiaminopimelate desuccinylase Wehrmann, A. et al. "Analysis of different DNA fragmnents of Corynebacterium glutamicumn complementing dapE of Escherichia coli," 40:3349-56 (1994) 2006200985 24 Feb 2006 Tal continued) X82061 16S rDNA 16S ribosomal RNA Ruimy, R. et at. "Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences," In. J. Syst. Bacteriol., (1995) X82928 asd; IysC Aspartate-semialdehyde dehydrogenase; Serebrijski, 1. et al. "Multicopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," J.

1 77(24):7255-7260 (1995) X82929 proA Gamma-glutamyl phosphate reductase Serebrijski, 1. et al. "Mutticopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," 1 77(24):7255-7260 (1995) X84257 16S rDNA 165 ribosomal RNA Pascual, C. et at. "Phytogenetic analysis of the genus Corynebacterium based 165 rRNA gene sequences," In. J Sys!. Bacteriol., 45(4):724-728 (1995) X85965 aroP; dapE Aromatic amino acid permease; Wehrmann et at. "Functional analysis of sequences adjacent to dapE of C.

glutamicum proline reveals the presence of aroP, which encodes the aromatic acid transporter," J. BacterioL, 177(20);5991-5993 (1995) X86157 argB; argC; argD; Acetylglutamnate kinase; N-acetyl-gamma- Sakanyan, V. et at. "Genes and enzymes of the acetyl cycle of arginine argF; argi glIutamyl -phosphate reductase; biosynthesis in Gorynebacterium glutamicum: enzyme evolution in the early acetylomithine aminotransferase; omnithine steps of the arginine pathway," Microbiology, 142:99-108 (1996) carbamoyltransferase; glutamate Nacety Itransferase X89084 pta; ackA Phosphate acetyltransferase; acetate kinase Reinscheid, D.J. et al. "Cloning, sequence analysis, expression and inactivation of the Corynebacterium glutam icum pta-ack operon encoding sacety lase and acetate kinase," Microbiology, 145:503-513 (1999) X89850 attB Attachment site Le Marrec, C. et al. "Genetic characterization of site-specific integration functions of phi AAU2 infecting "Arthrobacter aureus C70," J. Bacteriol., (1996) X90356 Promoter fragment FlI Patek, M. et at. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90357 Promoter fragment F2 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90358 Promoter fragment FI10 Patek, M. et at. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90359 Promoter fragment F13 Patek, M. et at. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) 2006200985 24 Feb 2006 2 (continued) X90360 Promoter fragment F22 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90361 Promoter fragment F34 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90362 Promoter fragment F37 Patek, M. et al. "Promoters from C. glutamicum: cloning, molecular analysis search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90363 Promoter fragment F45 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90364 Promoter fragment F64 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90365 Promoter fragment P75 Patek, M. et al. "Promoters from Corynebacterium glutainicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90366 Promoter fragment PPIOI Patek, M. et al. "Promoters from Corynebacterium glutaxnicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90367 Promoter fragment PP 104 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology (1996) X90368 Promoter fragment PFI109 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology (1996) X93513 amt Ammonium transport system Siewe, R.M. et al. "Functional and genetic characterization of the (methyl) ammoniunm uptake carrier of Corynebacterium glutamicum," J. Biol. Chem., 0):5398-5403 (1996) X93514 betP Glycine betaine transport system Peter, H. et al. "Isolation, characterization, and expression of the Gorynebacterium glutamicum betP gene, encoding the transport system for the solute glycine betaine," J Bacteriol., 178(17):5229-5234 (1996) X95649 orf4 Patek, M. et al. "Identification and transcriptional analysis of the dapB-ORP2dapA-ORF4 operon of Corynebacterium glutamicum, encoding two enzymes involved in L-lysine synthesis," Biolechnol. Leif., 19:1113-1117 (1997) X96471 lysE; lysG Lysine exporter protein; Lysine export Vrljic, M. et al. "A new type of transporter with a new type of cellular regulator protein function: L-lysine export from Corynebacterium glutamicum," Mot.

I Micro biol., 22(5):815-826 (1996) 2006200985 24 Feb 2006 Table 2 (continued) X96580 panB; panC; xylB 3-methyl-2-oxobutanoate Sahm, H. et "D-pantothenate synthesis in Corynebacteriumn glutamicum and hydroxymethyl trans ferase; pantoate-beta. use of panBC and genes encoding L-valine synthesis for D-pantothenate alanine ligase; xylulokinase overproduction," Appi. Environ. Microbiol., 65(5): 1973-1979 (1999) X96962 Insertion sequence IS 1207 and transposase X99289 Elongation factor P Ramos, A. et al. "Cloning, sequencing and expression of the gene encoding elongation factor P in the amino-acid producer Brevibacterium lactofermentum ___________(Corynebacterium glutamicum ATCC 13869)," Gene, 198:217-222 (1997) Y00 140 thrB Homoserine kinase Mateos, L.M. et al. "Nucleotide sequence of the homoserine kinase (thrB) gene the Brevibacterium lactofermentum," Nucleic Acids Res., 15(9):3922 (1987) Y00151 ddh Meso-diaminopimelate D-dehydrogenase Ishino, S. et al. "Nucleotide sequence of the meso-diaminopimelate D- (EC 1.4.1.16) dehydrogenase gene from Corynebacterium glutamicum," Nucleic Acids Res., 15(9):3917 (1987) Y00476 thrA Homoserine dehydrogenase Mateos, L.M. et al. "Nucleotide sequence of the homoserine dehydrogenase (thrA) gene of the Brevibacterium lactofermentum," Nucleic Acids Res., (1987) Y00546 horn; thrB H-omoserine dehydrogenase; homoserine Peoples, O.P. et al. "Nucleotide sequence and fine structural analysis of the kinase Corynebacterium glutamicumn hom-thrB operon," Mol. Microbiol., 2(1):63-72 Y08964 murC; ftsQfdivD; ftsZ UPD-N-acetylmurainate-alanine ligase; Honrubia, M.P. et al. "Identification, characterization, and chromosomal division initiation protein or cell division organization of the ftsZ gene from Brevibacterium lactofermentum," Mo!. Gen.

cell division protein Gene!., 259(l):97-104 (1998) Y09 163 putP High affinity proline transport system Peter, H. et al. "Isolation of the putP gene of Corynebacterium glutamicumproline and characterization of a low-affinity uptake system for solutes," Arch. Microbiol., 168(2): 143 -151 (1997) Y09548 pyc Pyruvate carboxylase Peters- Wend isch, P.G. et al. "Pyruvate carboxylase from Corynebacterium glutamicum: characterization, expression and inactivation of the pyc gene," icrobiology, 144:915-927 (1998) Y09578 leuB 3- isopropylma late dehydrogenase Patek, M. et al. "Analysis of the leuB gene from Corynebacterium App!. Microb jot. Riolechnol., 50(1):42-47 (1998) Y 12472 Attachment site bacteriophage Phi- 16 Moreau, S. et al. "Site-specific integration of corynephage Phi- 16: The ____________construction of an integration vector," Microbiol., 145:539-548 (1999) Y 12537 proP Proline/ectoine uptake system protein Peter, H. et al. "Corynebacterium glutamicum is equipped with four secondary carriers for compatible solutes: Identification, sequencing, and characterization of the proline/ectoine uptake system, Prop, and the ectoine/proline/glycine carrier, EctP," J Bacteriol., 180(22):6005-6012 (1998) 2006200985 24 Feb 2006 Table 2 (continued) Y 13221 gInA Glutamine synthetase I Jakoby, M. et al. "Isolation of Corynebacterium glutamicurn ginA gene encoding glutamine synthetase FEMS Microbiol Leif., 154(l):81-88 (1997) Y 16642 lpd Dihydrolipoamide dehydrogenase Y 18059 Attachment site Corynephage 304L Moreau, S. et al. "Analysis of the integration functions of φ304L: An module among corynephages," Virology, 255(l):150-159 (1999) Z21501 argS; lysA Arginyl-tRNA synthetase; diaminopimelate Oguiza, J.A. et al. "A gene encoding arginyl-tRNA synthetase is located in the decarboxylase (partial) upstream region of the lysA gene in Brevibacteriumn lactofermentum: Regulation of argS-lysA cluster expression by arginine," J.

1 75(22):7356-7362 (1993) Z21502 dapA; dapB Dihydrodipicolinate synthase; Pisabarro, A. et al. "A cluster of three genes (dapA, orf2, and dapB) of dihydrodipicolinate reductase Brevibacterium lactofermentumn encodes dihydrodipicolinate reductase, and a third polypeptide of unknown function," J. BacterioL, 175(9):2743-2749 Z29563 thrC Threonine synthase .Malumbres, M. et al. "Analysis and expression of the thrC gene of the encoded synthase," App!. Environ. Microbiol, 60(7)2209-22 19 (1994) Z46753 1 6S rDNA Gene for 1 6S ribosomal RNA Z49822 sigA SigA sigma factor Oguiza, J.A. et al "Multiple sigma factor genes in Brevibacteriumn lactofermentum: Characterization of sigA and sigB," J1 BacterioL, 178(2):550- 553 (1996) Z49823 galE; dtxR Catalytic activity UDP-gaiactose 4- Oguiza, J.A. et al "The galE gene encoding the UDP-galactose 4-epimerase of epimnerase; diphtheria toxin regulatory Brevibacterium lactofermentumn is coupled transcriptionally to the dmdR protein gene," Gene, 177:103-107 (1996) Z49824 orf I; sigB SigB sigma factor Oguiza, J.A. et al "Multiple sigma factor genes in Brevibacteriumn lactofermentum: Characterization of sigA and sigB," J BacterioL. 178(2):550- 53(996) Z66534 Transposase Correia, A. et al. "Cloning and characterization of an IS-like element present in the genome of Brevibacteriumn lactoflermentumn ATCC 13869," Gene, A sequence for this gene was published in the indicated reference. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.

86 TABLE 3: Corynebacterium and Brevibacterium Strains Which May be Used in the Practice of the Invention -FMcis~ RM Brevibacterium ammoniagenes 21054 Brevibacterium ammoniagenes 19350 Brevibacterium ammoniagenes 19351 Brevibacterium ammoniagenes 19352 Brevibacterium ammoniagenes .19353 Brevibacterium ammoniagenes 19354 Brevibacterium ammoniagenes 19355 Brevibacterium ammoniagenes 19356 Brevibacterium ammoniagenes 21055 Brevibacterium ammoniagenes 21077 Brevibacterium ammoniagenes 21553 Brevibacterium ammoniagenes 21580 Brevibacterium ammoniagenes 39101 Brevibacterium butanicum 21 196 Brevibacterium divaricatum 21792 P928 Brevibacterium tiavum 21474 Brevibacterium flavum 21129 Brevibacterium tiavum 21518 Brevibacterium flavum BI 11474 Brevibacterium flavum B 11472 Brevibacterium flavum 21127 Brevibacterium fiavum 21128 Brevibacterium flavum 21427 Brevibacterium flavum 21475 Brevibacterium fiavum 21517 Brevibacterium fiavum 21528 Brevibacterium flavum 21529 Brevibacterium flavum BI 11477 Brevibacterium flavum BI 1478 Brevibacterium flavum 21127 Brevibacterium flavum B 11474 Brevibacterium healii 15527 Brevibacterium ketoglutamicum 21004 Brevibacterium ketoglutamicum 21089 Brevibacterium ketosoreductum 21914 Brevibacterium lactofermentum Brevibacterium Iactofcrmentum 74 Brev ibacterium lactofermentum 77 Brevibacterium lactofermentum 21798 Brevibacterium lactofermentum 21799 Brevibacterium lactofermentum 21800 Brevibacterium lactofermentun, 21801 Brevibacterium lactofermentum BI 11470 Brevibacterium lactofermentum BI 1471 87 Geus seces-ATCC. FERNTNRRE. CECT. N!CIMB CBS NCTC SMZ Brevibacterium lactofermnentumn 21086 Brevibacterium lactofermentumn 21420 Brevibacterium lactofermentumn 21086 Brevibacterium lactofermentumn 31269 Brevibacterium linens 9174 Brevibacterium linens 19391 Brevibacterium linens 8377 Brev ibacterium paraffinolyticum Brevibacteriumn spec. Brevibacterium spec. Brevibacterium spec. 14604 Brevibacterium spec. 21860 Brevibacterium spec. 21864 Brevibacterium spec. 21865 Brevibacterium spec- 21866 Brevibacterium spec. 19240 Corynebacterium acetoacidophilumn 21476 Corynebacterium acetoacidophilumn 13870 Corynebacterium acetoglutamnicum B 11473 Corynebacterium acetoglutamnicumn B 1475 Corynebacterium acetoglutamnicumn 15806 Corynebacterium acetoglutamnicumn 21491 Corynebacterium acetoglutamnicumn 31270 Corynebacterium acetophilum B3671 Corynebacterium ammoniagenes 6872 2399 Corynebacterium ammoniagenes 15511 Corynebacterium fujiokense 21496 Corynebacterium glutamnicumn 14067 Corynebacterium glutamnicumn 39137 Corynebacterium glutamnicumn 21254 Corynebacterium glutamnicum 21255 Corynebacterium glutamnicumn 31830 Corynebacterium glutamnicumn 13032 Corynebacterium glutamnicumn 14305 Coryhebacteriumn glutamnicumn 15455 Corynebacterium glutamnicumn 13058 Corynebacterium glutamnicumn 13059 Corynebacterium glutamicum 13060 Corynebacterium glutamnicumn 21492 Corynebacterium glutamnicumn 21513 Corynebacterium glutamnicumn 21526 Corynebacterium glutamnicumn 21543 Cor-nebacterium glutamnicumn 13287 Corynebacterium glutamnicumn 21851 Corynebacterium glutamnicumn 21253 Corynebacterium glutamnicumn 21514 Corynebacterium glutamnicumn 21516 Corynebacterium glutamicumn 21299 -88z A T FER NRI CC-N MJ C K _kDSMZ Corynebacterium glutamnicum 21300 Corynebacterium glutamicum 39684 Corynebacterium glutamicum 21488 Coryriebacterium glutamicum 21649 Corynebacterium glutamicum 21650 Corynebacterium glutamicum 19223 Corynebacterium glutamicum 13869 Corynebacterium glutamicum 21157 Corynebacterium glutamicum 21158 Corynebacterium glutamicum 21159 Corynebacterium glutamicum 21355 Corynebacterium glutamicum 31808 Corynebacterium glutamicum 21674 Corynebacterium glutamicum 21562 Corynebacterium glutamicum 21563 Corynebacterium glutamicum 21564 Corynebacterium glutamicum 21565 Corynebacterium giutamicum 21566 Corynebacterium glutamicum 21567 Corynebacterium glutamicum 21568 Corynebacterium glutamicum 21569 Corynebacterium glutamicum 21570 Corynebacterium glutamicum 21571 Corynebacteriumn glutamicum 21572 Corynebacterium giutamicum 21573 Corynebacterium glutamicum 21579 Corynebacterium glutamicum 19049 Corynebacterium glutamicum 19050 Corynebacterium glutamicum 19051 Gorynebacterium glutamicum Corynebacterium glutamicum 19053 Corynebacteriuni giutamicum 19054 Corynebacterium glutamicum 19055 Corynebacterium glutamicum 19056 Corynebacterium glutamicum 19057 Corynebacterium giutamicum 19058 Corynebacterium giutamicum 19059 Corynebacteriuni glutamicum 19060 Corynebacteriuni glutamicum 19185 Corynebacterium glutamicum Corynebacteriuni glutamicum 21515 Corynebacterium glutamicum 21527 Corynebacterium giutamicum 21544 Corynebacterium giutamicum 21492 Corynebacterium glutamicum B8183 Corynebacterium giutarnicum B8182 Corynebacterium giutamicum B12416 Corynebacterium giutamicum B12417 89 Corynebacterium glutamicum Corynebacterium glutamicum 11476 Corynebacterium glutamicum 21608 Corynebacterium lilium P973 Corynebacterium nitrilophilus 21419 11594 Corynebacterium spec. P4445 Corynebactenriu spec. P4446 Corynebacterium spec. 31088 Corynebacterium spec. 31089 Corynebacterium spec. 31090 Corynebacterium spec. 31090 Corynebacterium spec. 31090 Corynebacterium spec. 15954 ___20145 Corynebacterium spec. 21857 Corynebacterium spec. 21862 Corynebacterium spec. 21863 ATCC: American Type Culture Collection, Rockville, MD, USA FERM: Fermentation Research Institute, Chiba, Japan NRRL: ARS Culture Collection, Northern Regional Research Laboratory, Peoria, IL, USA CECT: Coleccion Espanola de Cultivos Tipo, Valencia, Spain NCIMB: National Collection of Industrial and Marine Bacteria Ltd., Aberdeen, UK CBS: Centraalbureau voor Schimmelcultures, Baam, NL NCTC: National Collection of Type Cultures, London, UK DSMZ: Deutsche Samm lung von Mikroorganismen und Zelikulturen, Braunschweig, Germany For reference see Sugawara, et al. (1993) World directory of collections of cultures of microorganisms: Bacteria, fuingi and yeasts (411edn), World federation for culture collections world data center on microorganisms, Saimata, Japen.

2006200985 24 Feb 2006 Table 4: Alignment Results Length Accession Name of Genbank Hit ID It length Genbank Hit

(NT)

Source of Genbank Hit homology Date of (GAP) Deiposit 37.148 13-Jul-99 rxa00013 996 GBGSS4:AQ713475 581 AQ713475 GB-HTG3:AC007420 130583 AC007420 GBHTG3:AC007420 130583 AC007420 rxaOO0l4 903 GB-BAI:MTCY3A2 25830 Z83867 HS_5402_82_A1 2_T7A RPCI-i 1 Human Male BAC Library llama sapiens Homo sapiens genomic clone Plate=978 Col=24 Row--B, genomic survey sequence.

Drosophila melanogaster chromosome 2 clone BACRO7M1 0 (D630) RPCI-98 Drosophila melanogaster 07.M.10 map 24A-24D strain y; ci bow sp, **SEQUENCING IN PROGRESS unordered pieces.

Drosophila melanogaster chromosome 2 clone BACR07MI10 (D630) RPCI-98 Drosophila melanogaster 07.M.10 map 24A-240 strain y; cn bw sp, **SEQUENCING IN PROGRESS-, 83 unordered pieces.

34,568 34,568 20-Sep-99 20-Sep-99 GB BA1:MLCB1 779 43254 GB-BA1 :SAPURCLUS 9120 rxaOOO3O 513 GBEST21:C89713 767 Z98271 X92429 089713 rxaOO032 1632 GBE5T28:A1497294 GB-EST21 :092 167 GB-BA2:AFO1 0496 GBBA2:AFO1 8073 GB-BA2:AF045245 EM-PAT:E1 1760 GB PAT:1261 24 GBIN1 :LMFL5883 EM.YAT:E1 1760 484 A1497294 637 092167 189370 AF010496 Mycobacterium tuberculosis H37Rv complete genome; segment 136/162. Mycobacterium 58,140 tuberculosis Mycobacterium Ieprae cosmid B1779. Mycobacterium leprae 57.589 Salboniger riapH, pur7, purlO0, pur6, pur4, pur5 and pur3 genes. Streptomyces anulatus 55,667 C89713 Dictyostelium discoideum SS (H.Urushihara) Dictyostelium discoideum Dictyostelium discoideum 45,283 cDNA clone SSG229, mRNA sequence.

fb63g03.yl Zebrafish WashU MFIMG EST Danio rerio cDNA 5' similar to Danlo rerio 42,991 SW:AFP4_MYOQO P80961 ANTIFREEZE PROTEIN LS-1 2. mRNA sequence.

092167 Dictyostelium discoideum SS (H.Urushihara) Dictyostelium discoideum Dictyostelium discoideum 44,444 cDNA clone SSD179, mRNA sequence.

Rhodobacter capsulatus strain SB1003, partial genome. Rhodlobacter capsulatus 39.689 8-Aug-97 28-Feb-96 20-Apr-98 11-MAR-I1999 12-Jul-99 17-Jun-98 9810 AF018073 Rhodobacter sphaeroides operon regulator (smoC), periplasmic sorbitol-binding Rhodobacter sphaeroides 48,045 protein (smoE), sorbftoVmannitol transport inner membrane protein (smoF), sorbitollmannitol transport inner membrane protein (smoG). sorbitoVmannitol transport ATP-binding transport protein (smoK). sorbitol dlehydrogenase (smoS), mannitol dehydrogenase (mtlK), and periplasmic mannitol-binding protein (smoM) genes, complete cds.

5930 AF045245 Klebsiella pneumoniae D-arabinitol transporter (dalT) D-arabinitol kinase Klebsiella pneumoniae 38,514 (dalK), 0-arabinitol dehydrogenase (dalD), and repressor (daIR) genes, complete cds.

6911 El11760 Base sequence of sucrase gene. Corynebacterium 99,031 12-MAY-1 998 22-OCT-i 997 16-Jul-98 rxaOO04l 1342 08-OCT-i1997 glutarnicumn 6911 126124 Sequence 4 from patent US 5556776. Unknown.

31934 ALl 17384 Leishmania major Friedlin chromosome 23 cosmid L5883, complete sequence. Leishmania major 6911 El11760 Base sequence of sucrase gene. Corynebacterium glutamicum (Rel. 52, Created) 99,031 07-OCT-1 996 43,663 21-OCT-1999 94,767 08-OCT-i1997 (Rel. 52, Created) 94,767 07-OCT-i1996 rxaO42 882 GB-PAT-126124 6911 126124 Seune4fopaetU 5567.nkw. Sequence 4 from patent US 5556776. Unknown.

2006200985 24 Feb 2006 rxaOO043 1287 GOINI:CEU33OS1 GBPAT:126124 EMPAT:E1 1760 4899 U33051 6911 126124 6911 E11760 Table 4 (continued) Caenorhabditis elegans sur-2 mRNA, complete ccls.

Sequence 4 from patent US 5556776.

Base sequence of sucrase gene.

Homo sapiens clone UWGC:gI564a012 from 7p14-15, complete sequence.

Mycobacteriumn smegmatis phosphoglucose, isomerase gene, complete cds.

Caenorhabditis elegans Unknown.

Corynebacteriumn glutamicumn 40,276 97.591 97,591 GBPR3:AC005174 39769 rxaOOO98 1743 GB-BA1 :MSU88433 1928 GB-BA1 :SC5A7 40337 GBBA1:MTCYIOD7 39800 rxa00148 2334 GBBA1 :MTCY277 38300 GBBA1:MSGY456 37316 GB-BA1:MSGY175 18106 =x00149 1971 GBBA1:MSGY456 37316 GBBA1:MSGYI75 18106 GB-BA1:MTCY277 38300 684 GBBA1:MTCY274 39991 GBBA1:MSGB1529CS 36985 GBBAl :MTCY274 39991 rxaOO196 738 GBBA1:MTCY274 39991 GB-BA1:MTCY274 39991 GBRO:RATCBRQ 10752 rxaOO202 1065 GB-EST11I:AA253618 313 GB-EST26AI390284 490 AC0051 74 U88433 AL03i 107 Z79700 Z79701 ADOOOOO11 ADOOOlS1 ADOOOOO11 AD00001 5 Z79701 Z74024 L78824 Z74024 Z74024 Z74024 M55532 AA253618 A1390284 A1390280 Z99263 AL021 287 Homo sapiens 35,879 Mycobacterium smegmatis 62,658 Streptomyces coelicolor cosmid 5A7. Streptomyces coelicolor Mycobacterium tuberculosis H37Rv complete genome: segment 441162. Mycobacterium tuberculosis Mycobacteriumn tuberculosis H37Rv complete genome: segment 65/162. Mycobacteriurn tuberculosis Mycobacteriumn tuberculosis sequence from clone y 4 56. Mycobacterium tuberculosis Mycobacterium tuberculosis sequence from clone y1 7 5. Mycobacteriumn tuberculosis Mycobacterium tuberculosis sequence from clone y456. Mycobacteriumn tuberculosis Mycobacterium tuberculosis sequence from clone y175. Mycobacterium tuberculosis Mycobacteriumn tuberculosis H37Rv complete genome; segment 65/1 62. Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genome; segment 1261162. Mycobacterium tuberculosis Mycobacterium leprae cosmid B1529 DNA sequence. Mycobacterium leprae Mycobacteriumn tuberculosis H37Rv complete genome; segment 1261162. Mycobactenumr tuberculosis Mycobacterium tuberculosis H37Rv complete genome: segment 126/162. Mycobacteriumn tuberculosis Mycobacterium tuberculosis H37Rv complete genome; segment 126/162. Mycobacterium tuberculosis Rat carbohydrate binding receptor gene, complete cds. Rattus norvegicus mw95c1O.rI Soares mouse NMVL Mus musculus cDNA clone IMAGE:678450 Mus musculus mRNA sequence.

mwg6a03.yl Soares mouse NMVL Mus musculus cDNA clone IMAGE:678508 5 Mus musculus similar to TR:009171 009171 BETAINE-KOMOCYSTEINE METHYLTRANSFERASE;, mRNA sequence.

mw9Scl O.ylI Sopares mouse NMVL M us myusculus cDNA clone IMAGE:678450 Mus musculus mRNA sequence.

Mycobacterium leprae cosmid 8637. Mycobacteriumn leprae Mycobacteriumn tuberculosis H37Rv complete genome; segment 132/162. Mycobacterium tuberculosis 37,638 36.784 67.457 40,883 67,457 35,883 51,001 51,001 35,735 57,014 41,892 41,841 36,599 36,212 38,816 42,239 37.307 58,312 36,632 23-Jan-96 07-OCT-1 996 08-OCT-I1997 (Rel. 52, Created) 24-Jun-98 19-Apr-97 27-Jul-98 17-Jun-98 17-Jun-98 03-DEC-1 996 10-DEC-i1996 03-DEC-i1996 10-DEC-1996 17-Jun-98 19-Jun-98 1 5-Jun-96 19-Jun-98 19-Jun-98 19-Jun-98 27-Apr-93 1 3-MAR-i1997 2-Feb-99 GB-EST26:A1390280 GB BA1 :MLCB637 GB-BA1 :MTVO1 2 467 44882 70287 2-Feb-99 17-Sep-97 23-Jun-99 rxa0O206 1161 2006200985 24 Feb 2006 GBBA1 :SC6E1O rxa00224 1074 GBBAl ;BJU32230 GB-BAl:PDEETFAB GBHTG3:AC009689 rxa00225 909 GBRO:AF060178 GB.GSS1 1 :AQ325043 GBEST3l:A1876413 rxa00235 1398 GB3BA1:MTCY1OG2 GB-BA2:AF061753 GBBA2:AF086791 23990 1769 2440 177954 2057 734 551 38970 3721 37867 2690 1506 1688 37218 69350 69350 1038 469 7887 313 AL1 09661 U32230 L14864 AC009689 AF0601 78 AQ325043 A1676413 Z92539 AF061 753 AF086791 AF01 2550 E03856 D90421 Z77162 AL0091 98 AL0091 98 AF0501 14 B16984 AF144549 T28483 Table 4 (continued) Streptomyces coelicolor cosmid 6E 10. Streptomyces coelicolor 38,616 A3(2) Bradyrhizobium japonicum electron transfer flavoprotein small subunit (etfS) nd Bradyrhizobium japonicum 48,038 large subunit (etfL) genes, complete cdls.

Paracoccus denitrificans electron transfer flavoprotein alpha and beta subunit Paracoccus denitrificans 48,351 genes, complete cdss.

Homo sapiens chromosome 4 clone 104F.7 map 4, LOW-PASS SEQUENCE Homo sapiens 38,756

SAMPLING.

Mus musculus heparan sulfate 2-sulfotransferase (Hs2st) mRNA, complete cds.Mus musculus 39,506 mgxbOO2OJ0l r CUGI Rice Blast BAC Library Magnaporthe grisea genomic Magnaporthe grisea 38,333 clone mgxbOO2OJ01r, genomic survey sequence.

etmEST0l67 EtHi Elmeria tenella cDNA clone etmcO74 mRNA sequence. Eimeria tenella 35,542 Mycobacterium tuberculosis H37Rv complete genome; segment 47/162. Mycobacterium 65,759 tuberculosis Nitrosomonas europaea CTP synthase (pyrG) gene, partial cds; and enolase Nitrosomonas europaea 58,941 (eno) gene, complete cds.

Zymomonas mobilis strain ZM4 clone 67E10 carbamnoylphosphate synthetase Zymomonas mobilis 61 .239 small subunit (carA), carbanylphosphate synthetase large subunit (carB).

transcription elongation factor (greA), enolase (eno), pyruvate dlehydrogenase alpha subunit (pdhA), pyruvate dehydrogenase beta subunit (pdhB), ribonuclease H (rnh), homoserine kinase homolog, alcohol dlehydrogenase 11 (adhB), and excinuclease ABC subunit A (uvrA) genes, complete cdls; and unknown genes.

Acinetobacter sp. B0413 ComP (camP) gene, complete cdls. Acinetobacter sp. BD413 53,726 gONA encoding alcohol dehydrogenase. Bacillus 51,688 stearothermophilus B.stearothermophilus adhT gene for alcohol dlehydrogenase. Bacillus 51,602 stearothermophilus Mycobacterium tuberculosis H37Rv complete genome; segment 25/1 62. Mycobacterium 42,875 tuberculosis Mycobacterium tuberculosis H-37Rv complete genome; segment 144/1 62. Mycobacterium 40,380 tuberculosis Mycobacterium tuberculosis H37Rv complete genome; segment 144/1 62. Mycobacterium 41,769 tuberculosis Pseudomonas sp. W7 alginate lyase gene, complete cdls. Pseudomonas sp. W7 49,898 344A14.TVC CIT978SKAI Homo sapiens genomic clone A-344A14, genomic Homo sapiens 39,355 survey sequence.

Aedes albopictus ribosomal protein L34 (rpl34) gene, complete cds. Aedes albopictus 36,509 EST46182 Human Kidney Homo sapiens cONA 3'end similar to flavin- Homo sapiens 42,997 containing monooxygenase 1 (HT:1 956), mRNA sequence.

5-Aug-99 25-MAY-I 996 27-OCr-1 993 28-Aug-99 1 8-Jun-98 8-Jan-99 19-MAY-I1999 17-Jun-98 31-Aug-98 4-Nov-98 27-Sep-99 29-Sep-97 7-Feb-99 17-Jun-98 18-Jun-98 18-Jun-98 03-MAR-1 999 4-Jun-98 3-Jun-99 6-Sep-95 rxa00246 1158 GBBA2:AF0l25SO GB,,PAT:E03856 GB_BAl :BACADHT rxaGO251 831 GBBA1:MTCY2OG9 GB_BA1:MTVOD4 GB-BA1:MTVOO4 rxa00288 1134 GB _BA2:AF0501114 GB_GSS3:B16984 GB13 N2:AF144549 rxa00293 1035 GB-EST1:T28483 2006200985 24 Feb 2006 GBPR:-UMFMO1 2134 M64082 GB-EST32:A1734238 512 A1734238 GBHTG6:AC011069 168266 AC011069 rxa00296 2967 GB_EST1 5:AA531468 GBHTG6:AC011069 rxaOO31 0 558 GB-VI:VMVY1 6780 GB I:VARCG

GB_VI:WVCGPA

rxa00317 777 GB-HTG3:AC009571 GB-HTG3:AC009571 GBPR3:AC005697 rxa00327 507 GBBAl :LCATPASEB GB_BA1:LCATPASEB rxa00328 615 GB_BA1:STYPUTPE GBBAl :SrYPUTPF GB_BAi:STYPUTPI rxa00329 1347 GBPR3:AC004691 GB PR4A0004916 GB-PR3:AC004691 rxaOO340 1269 GB GAl :MTCY427 GB-GSS1 2:AQ412290 GB PL2:AF1 12871 rxa00379 307 GBHTG1 :CEY56A3 GB-HTG1 :CEY56A3 414 168266 AA531468 AC01 1069 186986 Y16780 186103 L22579 185578 X69198 159648 AC009571 159648 AC009571 174503 AC005697 1514 X64542 1514 X64542 1887 L01138 1887 L01 139 1889 L01142 141990 AC004691 129014 AC004916 141990 AC004691 38110 Z70692 238 AQ412290 2394 AF112871 224746 AL022280 224746 AL022280 Table 4 (continued) Human flavin-containing monooxygenase (FMO1) mRNA, complete cdls.

zb73c05.y5 Soares fetal lung.NbHL1 9W Homo sapiens cDNA clone IMAGE:309224 5 similr to gb:M64082 DIMETHYLANILINE MONOOXYGENASE (HUMAN);, mRNA sequence.

Drosophila melanogaster chromosome X clone BACR1 1H20 (0881) RPCI-98 11.1-.20 map 12B3-12C strain y; cn bw sp, **SEQUENCING IN PROGRESS 92 unordered pieces.

nj63d12.sl NCItCGAP_PrlO0 Homo sapiens cONA clone IMAGE:997175.

mRNA sequence.

Drosophila melanogaster chromosome X clone BACR1 1H20 (D881) RPCI-98 11.1-.20 map 1213-12C strain y; cn bw sp, SEQUENCING IN PROGRESS 92 unordered pieces.

variola minor virus complete genome.

Variola major virus (strain Bangladesh-i 975) complete genome.

Variola virus DNA complete genonne.

Homo sapiens chromosome 4 clone 57 -A 22 map 4, SEQUENCING IN PROGRESS unordered pieces.

Homo sapiens chromosome 4 clone 57 -A -22 map 4, 'SEQUENCING IN PROGRESS 8 unordered pieces.

Homo sapiens chromosome 17, done hRPK.138_P_22, complete sequence.

Lecasei gene for ATPase beta-subunit.

L.casei gene for ATPase beta-subunit.

Salmonella (S2980) proline permease (putP) gene, 5Fend.

Salmonella (S2983) proline permease (putP) gene, 5 end.

Salmonella (S3015) proline permease (putP) gene. 5 end.

Homo sapiens PAC clone 0.1074002 from 7p14-p1 5, complete sequence.

Homo sapiens clone DJ0891 11 4, complete sequence.

Homo sapiens PAC clone D.107400302 from 7p14-p15, complete sequence.

Mycobacterium tuberculosis H-37Rv complete genome; segment 99/162.

RPCI-1 1-195H2.TV RPCI-1 1 Homo sapiens genomic clone RPCI-1 1-1951-2, genomic survey sequence.

Astasia longa small subunit ribosomal RNA gene, complete sequence.

Caenorhabditis elegans chromosome Ill clone Y56A3, SEQUENCING IN PROGRESS in unordered pieces.

Caenorhabditis elegans chromosome III clone Y56A3, SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens Homo sapiens 37,915 8-Nov-94 41,502 14-Jun-99 Drosophila melanogaster 33,890 Homo sapiens 40,821 Drosophila melanogaster 30,963 variola minor virus 35,883 Variola major virus 34,664 Variola virus 36,000 Homo sapiens 36,988 Homo sapiens 36,988 Homo sapiens 36,340 Lactobacillus casei 34,664 Lactobacillus casel 39,308 Salmonella sp. 39,623 Salmonella sp. 39,623 Salmonella sp. 42,906 Homo sapiens 38,142 Homo sapiens 38,549 Homo sapiens 35,865 Mycobacterium 38,940 tuberculosis Homo sapiens 36,555 Astasia longa 36,465 Caenorhabditis elegans 35,179 Caenorhabditis elegans 35,179 02-DEC-1 999 20-Aug-97 02-DEC-i 999 2-Sep-99 12-Jan-95 1 3-DEC-i1998 29-Sep-99 29-Sep-99 09-OCT-i 998 11-DEC-i1992 11-DEC-1992 09-MAY-i1996 094MAY-1996 09-MAY-i1996 16-MAY-i1998 17-Jul-99 16-MAY-i 998 24-Jun-99 23-MAR-1 999 28-Jun-99 6-Sep-99 6-Sep-99 2006200985 24 Feb 2006 GBPR2:HS134019 rxaOO38l 729 GB-GSS4:AQ730532 GBE5T23:A1120939 GBEST23:A1120939 rxa00385 362 GBE5T32:A1726450 GB-GSS4:AQ740856 GBjPR1 :HSPAIP rxa00388 1134 GBBA1:MTY25D1O GBBA1:MSGY224 GB-HTG1 :AP000471 rxa00427 909 GBBA1:MSGYI26 GB8BA1:MTY1 3012 GB-HTG1:CEY48C3 rxa00483 1587 GBPR2:HSAFOO1S5O GBBAi:LLCPJW565 GBHTG2:AC006754 rxaOO511 615 GBPR3:HSE127C11 GB-PR3:HSE127C1 1 rxa00512 718 GBBA1:MTCY22G8 86897 416 561 561 565 768 1587 40838 40051 72466 37164 37085 270193 173882 1282B 206217 38423 38423 22550 Table 4 (continued) AL034555 Human DNA sequence from clone 134019 on chromosome I p36.11-36.33. Homo sapiens complete sequence.

AQ730532 HS_2149_Al_006_1-70 CIT Approved Human Genomic Sperm Library D Homo sapiens Homo sapiens genomic clone Plate=2149 001=1 1 Row-E, genomic survey sequence.

A1120939 ub74f05.r1 Soares mouse mammary gland NMLMG Mus musculus cONA clone Mus musculus IMAGE:1383489 5' similar to gb:J04046 CALMODULIN (HUMAN): gb:M19381 Mouse calmodulin (MOUSE);, mRNA sequence.

A1120939 ub74f05.rl Soares mouse mammary gland NMLMG Mus musculus cDNA clone Mus musculus 40,604 23-Nov-99 35,766 15-Jul-99 41,113 2-Sep-98 41,113 2-Sep-98 A1726450 AQ740856 X91809 Z95558 AD000004 AP000471 ADOOD0l 2 Z80343 Z92855 AF001 550 Y12736 AC006754 Z74581 Z74581 Z95565 IMAGE:1383489 5' similar to gb:J04046 CALMODULIN (HUMAN); gb:M19381 Mouse calmodulin (MOUSE);, mRNA sequence.

BNLGHi5857 Six-day Cotton fiber Gossypium hirsutumn cDNA 5' similar to (AF0l 5913) Skbl1ls [Homo sapiens], mRNA sequence.

HS_2274_A2_,A07_1 70 CIT Approved Human Genomic Sperm Library 0 Homo sapiens genomic clone Plate=2274 Col=14 Row--A. genomic survey sequence.

H.sapiens mRNA for GAIP protein.

Mycoboacterium tuberculosis H37Rv complete genome: segment 28/162.

Mycobacterium tuberculosis sequence from clone y224.

Homo sapiens chromosome 21 clone B2308H15 map 21q22.3, SEQUENCING IN PROGRESS in unordered pieces.

Mycobacterium tuberculosis sequence from clone y126.

Mycobacterium tuberculosis H-37Rv complete genome; segment 156/1 62.

Caenorhabditis elegans chromosome 11 clone Y48C3, SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome 16 BAC clone CIT987SK-334DI 1 complete sequence.

Lactococcus lactis. cremoris plasmid pJW565 DNA, abiiM. abiiR genes and orfX.

Caenorhabditis elegans clone Y40111, SEQUENCING IN PROGRESS unordered pieces.

Human DNA sequence from cosmid El127C11I on chromosome 22q1 1.2-qter contains STS.

Human DNA sequence from cosmid El 2701 1 on chromosome 22q1 1.2-qter contains STS.

Mycobactedriu tuberculosis H37Rv complete genome; segment 49/162.

Gossypium hirsutum Homo sapiens Homo sapiens Mycobacterium tuberculosis Mycobacteriumn tuberculosis Homo sapiens Mycobacterium tuberculosis Mycobacterlum tuberculosis Caenorhabditis elegans Homo sapiens Lactococcus lactis subsp.

cremoris Caenorhabditis elegans Homo sapiens Homo sapiens Mycobacterium tuberculosis 41,152 41,360 36,792 51,852 51,852 36,875 60,022 60,022 28,013 38,226 37.492 36,648 39,831 36,409 56,232 11 -Jun-99 16-Jul-99 29-MAR-1 996 17-Jun-98 03-DEC-i1996 13-Sep-99 10-DEC-i 996 17-Jun-98 29-MAY-1999 22-Aug-97 01-MAR-i1999 23-Feb-99 23-Nov-99 23-Nov-99 17-Jun-98 2006200985 24 Feb 2006 Table 4 (continued) M.smegmatis gitA gene for citrate synthase. GBBAI:MSGLTA 1776 X60513 Mycobacterium smegmatis 56,143 20-Sep-91 GBBA2:ECU73857 rxa00517 1164 GBHTG2:AC006911 GBHTG2:AC00691 1 GB-EST29:A16021 58 rxa00518 320 GB BA2:ECU73857 GBBA2:STU51879 rxa00606 2378 rxa00635 1860 GB BA2:AE0001 40 GBEST32:AU068253 OBESTI 3:AA363046 GB-EST32:AU068253 GBBA1:PAORF1

GBBAI:PAORFI

128824 298804 298804 481 128824 8371 12498 376 329 376 1440 1440 80381 81493 80381 38400 81493 43481 197110 197110 U73857 AC006911 AC00691 1 A16021 58 U73857 U51879 AE000140 AU068253 AA363046 AU068253 X 13378 X1 3378 AC01 0871 X98130 AC01 0871 AC004058 X98130 AB026648 AC01 0325 AC01 0325 Escherichia coli chromosome minutes 6-8.

Caenorhabditis elegans clone Y94H6x, ~SEQUENCING IN PROGRESS***, Caenorhabditis elegans unordered pieces.

Caenorhabditis elegans clone Y94H6x, **SEQUENCING IN PROGRESS Caenorhabditis elegans unordered pieces.

UI-R-ABO-vy-a-01-0-UI.s2 UI-R-AB0 Rattus norvegicus cDNA clone UI-R-ABO- Rattus norvegicus vy-a-01-0-UI mRNA sequence.

Eschericliia ccli chromosome minutes 6-8. Escherichia coli Salmonella lyphimurium propionate catabolism operon: RpoN activator protein Salmonella Lyphimurium homolog (prpR), carboxyphosphonoenolpyruvate phosphonomnutase homolog (prpB), citrate synthase homolog (prpC), prpD) and prpE genes, complete cds.

Escherichia ccli K-i 2 MG1 655 section 30 of 400 of the complete genome. Escherichia coli AU068253 Rice callus Oryza sativa cDNA clone 012658_9A, mRNA sequence. Oryza sativa EST72922 Ovary 11 Homo sapiens cIDNA 5Send. mRNA sequence. Homo sapiens AU068253 Rice callus Oryza sativa cDNA clone 012658 9A, mIRNA sequence. Oryza sativa Pseudomonas amyloderamosa DNA for ORF 1. P'seudomonas amyloderamosa Pseudomonas amnyloderamosa DNA for ORF 1. Pseudomon2s amyloderamosa Arabidlopsis thaliana chromosome III BAC T1601 1 genomic sequence, Arabidopsis thaliana complete sequence.

Athaliana 81kb genomic sequence. Arabidopsis thaliana Arabidopsis thaliana chromosome IIl BAC Ti 6011 genomic sequence, Arabidopsis thaliana complete sequence.

Homo sapiens chromosome 4 done B241 P19 map 4q25, complete sequence. Homo sapiens Athaliana 81kb genomic sequence. Arabidlopsis thaliana Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone: MLJ15, complete Arabidopsis thaliana sequence.

Homo sapiens chromosome 19 clone CITB-E1_2568A17, -SEQUENCING IN Homo sapiens PROGRESS 40 unordered pieces.

Home sapiens chromosome 19 clone CITB-El12568A1 7, -SEQUENCING IN Homo sapiens PROGRESS 40 unordered pieces.

Home sapiens dlone NH0576F01, complete sequence. Home sapiens 37,889 37,889 40,833 49,688 50,313 49,688 41,333 34,347 41,899 53,912 54,422 38,244 36.091 37,135 36,165 38,732 38,732 37,976 37,976 24-Feb-99 24-Feb-99 21-Apr.99 1 4-Jul-99 5-Aug-99 12-Nov-98 7-Jun-99 21-Apr-97 7-Jun-99 14-Jul-95 14-Jul-95 Escherichia coli 48,563 14-Jul-99 rxa00679 1389 GBPL2:AC010871 GB-PL1 :AT8I KBGEN GB-PL2:ACO1 0871 rxaOO68O 441 GBPR3:AC004058 GBPLI:AT81KBGEN GBPLIAB026648 rxa00682 2022 GBHTG3:ACO1 0325 GBHTG3:ACO1 0325 13-Nov-99 12-MAR-i 997 1 3-Nov-99 30-Sep-98 1 2-MAR-i1997 07-MAY-i1999 15-Sep-99 15-Sep-99 GB-PR4:AC008179 181745 ACC08179 37,143 28-Sep-99 2006200985 24 Feb 2006 rxaG0683 1215 GB_6A2:AE000896 GBIN1:DMBR7A4 GB-EST35:AV1 63010 rxa00686 927 GB-HTG2:HSDJ1 37K2 GB-HTG2:I-SDJI 37K2 GBEST12:AA284399 rxa00700 927 GBEST34:A1785570 GBEST25:A1256147 10707 212734 273 190223 190223 431 454 AE000896 AL109630 AV163010 AL049820 AL049820 AA284399 A1785570 Table 4 (continued) Methanobacterium thermoautotrophicum from bases 1189349 to 1200055 (section 102 of 148) of the complete genome.

Drosophila melanogaster clone BACR7A4.

AVI 63010 Mus musculus head C578L16J 13-day embryo Mus musculus cONA clone 3110006J22, mRNA sequence.

Homo sapiens chromosome 6 clone RPI-137K2 map q25.1-25.3.

SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome 6 done RPI -1 37K2 map q25.1-25.3, SEQUENCING IN PROGRESS in unordered pieces.

zs57b04.rl NCI CGAP_GCB1 Homo sapiens cDNA clone IMAGE:701551 5', mRNA sequence.

ui44d03.xl Sugano mouse liver mlia Mus musculus cDNA dlone IMAGE:1922789 3' similar to gb:Z28407 60S RIBOSOMAL PROTEIN L8 (HUMAN):. mRNA sequence.

ui95e12.xl Sugano mouse liver mlia Mus musculus cDNA clone IMAGE:1 890190 3 similar to gb:Z28.407 60S RIBOSOMAL PROTEIN L8 (HUMAN);, mRNA sequence.

C. aurantiacus reaction center genes 1 and 2.

Streptomyces coelicolor cosmid 7H-2.

Mycobacterium tuberculosis H37Rv complete genome; segment 126/162.

Methanobacterium thermoautotrophicum Drosophila melanogaster Mus musculus Homo sapiens Homo sapiens Homo sapiens Mus musculus Mus musculus Chloroflexus aurantiacus Streptomyces coelicolor A3(2) Mycobacterium tuberculosis 684 A1256147 rxaOO703 2409 rxaOO705 1038 rxa00782 1005 rxa00783 1395 rxa00794 1128 GBBA1:CARCG12 GB-BA1 :SC7H2 GBBAl :MTCY274 GBBA2:REU60056 GBGSS15:AQ604477 GB_ESTi 1 :AA224340 GB-EST5:N30648 GB GAl :MTCY1OD7 GBBA1:MLCL373 GBBA2:AF128399 GBHTG2:AC008158 GBHTG2:ACOO8I58 GB-PR3:ACO5O1 7 GB-BA1 :MTVO17 2079 42655 39991 2520 505 X14979 AL1 09732 Z74024 U60056 A0604477 38,429 36,454 41,758 38,031 38,031 39,205 41,943 40,791 37,721 56,646 37,369 51,087 39,817 35.129 43,986 63,327 62.300 53.698 35,135 35,135 35,864 40,331 15-Nov-97 30-Jul-99 8-Jul-99 03-DEC-i1999 03-DEC-i1999 14-Aug-97 2-Jul-99 12-Nov-98 23-Apr-91 2-Aug-99 19-Jun-98 16-OCT-i1996 10-Jun-99 1 1-MAR-1998 5-Jan-98 17-Jun-98 27-Aug-99 25-MAR-1 999 28-Jul-99 28-Jul-99 8-Aug-98 24-Jun-99 443 AA224340 291 N30648 39800 Z79700 37304 AL035500 2842 AF128399 118792 AC008158 118792 AC008158 137176 AC005017 67200 AL021897 Ralstonia eutropha formate dehydrogenase-like protein (cbbBe) gene, complete Raistonia eutropha cds.

HS_2116_81_G07_MR CIT Approved Human Genomic Sperm Library D Homo H-omo sapiens sapiens genomic clone Plate=21 16 CoI=1 3 Row=N, genomic survey sequence.

zrl4eO7.sl Stratagene hNT neuron (#937233) Homo sapiens cDNA clone Homo sapiens IMAGE:648804 3, mRNA sequence.

yw77b02.sl Soaresplacenta to9weeks-2NbHPBto9W Homo sapiens cDNA Homo sapiens dlone IMAGE:258219 mRNA sequence.

Mycobacterium tuberculosis H-37Rv complete genome; segment 44/162. Mycobacterium tuberculosis Mycobacterium Ieprae cosmid L373. Mycobacterium Iepr Pseudomonas aeruginosa succinyl-CoA synthetase beta subunit (sucC) and Pseudomonas aeru succinyl-CoA synthetase alpha subunit (sucD) genes, complete cds.

Homo sapiens chromosome 17 clone hRPK.42 -20 map 17, Homo sapiens SEQUENCING IN PROGRESS 14 unordered pieces.

Homo sapiens chromosome 17 clone InRPK.42JF20 map 17, Homo sapiens SEQUENCING IN PROGRESS 14 unordered pieces.

Homo sapiens BAC dlone GS214N 13 from 7p1 4-p1 5, complete sequence. Homo sapiens Mycobacterium tuberculosis H37Rv complete genome; segment 481162. Mycobacterium tuberculosis ae gmnosa 2006200985 24 Feb 2006 GB EA1:MLCB1222 34714 AL049491 GBPR2:HS151B14 128942 Z82188 rxa00799 1767 rxa00800 1227 rxa00825 1056 GBPL2:AF016327 616 GBHTG2:I-SDJ319M7 128208 GB-HTG2:HSDJ31 9M7 128208 GBB3A1:MTV022 G6,BA1 ABOl 9513 GBPL1 :SCSFAARP GB_BA1:MTY15C1O GB.8A1 :MLCB2548 GBBA2:AF1 69031 13025 4417 7008 33050 38916 1141 AF016327 AL079341 AL079341 AL021 925 AB01 9513 X68020 Z95436 AL023093 AF169031 Table 4 (continued) Mycobacterium leprae cosmid 131222.

Human DNA sequence from clone 1511B314 on chromosome 22 Contains SOMATOSTATIN RECEPTOR TYPE 3 (SS3R) gene,pseudlogene similar to ribosomal protein L39,RAC2 (RAS-RELATED C3 BOTULINUM TOXIN SUBSTRATE 2 (P21-RAC2)) gene ESTs, STSs. GSSs and CpG islands, complete sequence.

Hordeum vulgare Barpermi (permi) mRNA, partial cds.

Homo sapiens chromosome 6 clone RP1-319M7 map p21.1-21.3.

SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome 6 clone RP1-319M7 map p21.1-21.3, SEQUENCING IN PROGRESS in unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome: segment 100/162.

Streptomyces coelicolor genes for alcohol dehydrogenase and ABC transporter, complete eds.

S.cerevisiae SFA and ARP genes.

Mycobacterium tuberculosis H37Rv complete genome; segment 1541162.

Mycobacterium Ieprae cosmid 62548.

Xanthomonas oryzae pv. oryzae putative sugar nucleotide epimerase/dehydratase gene, partial cds.

Caenorhabditis elegans cosmid F23H12, complete sequence.

Homo sapiens chromosome 14 clone BAC 79J20 map 14q31, SEQUENCING IN PROGRESS 5 ordered pieces.

Homo sapiens chromosome 14 clone BAC 79J20 map 14q31, SEQUENCING IN PROGRESS ordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 811162.

Candida dlubliniensis ACTi gene, exons 1-2.

Candida albicans acti gene for actin.

Rhodobacter capsulatus strain SBIOO3, partial genome.

Rhizoblumn meliloti pha[AB,C,D,E,F,G] genes.

C23528 Japanese flounder spleen Paralichthys olivaceus cDNA clone HB5(2), mRNA sequence.

Homo sapiens chromosome 18 clone hRPK.44_-0_1 map 18, SEQUENCING IN PROGRESS 18 unordered pieces.

Mycobacterium Ieprae Homo sapiens Hordeum vulgare Homo sapiens Homo sapiens Mycobacterium tuberculosis Streptomyces coelicolor Saccharomyces cerevisiae Mycobacterium tuberculosis Mycobacterium Ieprae Xanthomonas oryzae pv.

oryzae Caenorhabditis elegans Homo sapiens Homo sapiens Mycobacterium tuberculosis Candida dubliniensis Candida albicans Rhodobacter capsulatus Sinorhizobium meliloti Paralichthys olivaceus Homo sapiens 41,311 36,845 36,845 63.101 41,312 36,288 39,980 39,435 46,232 61,170 27-Aug-99 37,455 16-Jun-99 01 -OCT-i1997 30-Nov-99 30-Nov-99 1 7-Jun-gB 13-Nov-98 29-Nov-94 17-Jun-98 27-Aug-99 14-Sep-99 rxa00871 rxa00872 1077 GB_IN1:CEF23H12 GB-HTG2:AC007263 GBHTG2:AC007263 rxa00879 2241 GB_BA1:M1V049 GBPL2:C0U236897 GB...PL1 :CMCT1A rxaOO909 955 GB-BA2:AF010496 GB-BA1 :RMPHA GB-EST16:C23528 rxaOO91 3 2118 GB-HTG2:AC007734 35564 167390 167390 40360 1827 3206 189370 7888 317 188267 Z74472 AC007263 AC007263 AL022021 AJ236897 X16377 AF010496 X93358 C23528 AC007734 34,502 35,714 35,714 36.981 38,716 36,610 51,586 48,367 41,640 34,457 08-OCT-1999 24-MAY-I1999 24-MAY-i1999 19-Jun-98 1-Sep-99 10O-Ap r-93 12-MAY-i1998 1 2-MAR-i1999 28-Sep-99 5-Jun-99 2006200985 24 Feb 2006 GB-HTG2:AC007734 GB-ESTI 8:AA709478 rxa00945 1095 GBHTG4:AC010351 GB-HTG4:ACO1 0351 GB-BA1 MTCY05A6 188267 406 220710 220710 38631 AC007734 AA709478 AC01103511 AC01 0351 Z96072 Table 4 (continued) Homo sapiens chromosome 18 clone hRPK.44_-0_-1 map 18, SEQUENCING IN PROGRESS 18 unordered pieces.

vv34a05.rl Stratagene mouse heart (#937316) Mus musculus cDNA clone IMAGE: 1224272 mRNA sequence.

Homo sapiens chromosome 5 clone CITB-I-1_2022136, SEQUENCING IN PROGRESS 68 unordered pieces.

Homo sapiens chromosome 5 clone CITB-H1_202286, SEQUENCING IN PROGRESS 68 unordered pieces.

Mycobactenumn tuberculosis H37Rv complete genome: segment 1201162.

Homo sapiens Mus musculus H-omo sapiens Homo sapiens Mycobacterium tuberculosis 34,457 42,065 36,448 36,448 36,218 5-Jun-99 24-DEC-1997 31-OCT-1999 31-OCT-1999 17-Jun-98 rxa00965 rxa00999 1575 GBPAT:E13660 GB8BA1:MTCY359 GBBA1:MLC81788 rxa0lO1S 442 GBBAI:MTVOO8 GBBA1:M1VOO8 1916 36021 39228 63033 63033 rxa0lO2S 1119 GBBA1:SC7A1 32039 GB-BA1:MSGB1723CS 38477 GB GAl :MLCB637 44882 rxa01048 1347 GBBA2:AF017444 3067 GB-BA1 :BSU80013 218470 GBYI:HSV2HG52 154746 rxa0lO49 1605 GBHTG2:AC002518 131855 GBHTG2:AC002518 131855 GBHTG2:AC002518 131855 rxa0lO7l 1494 GB-PR3:HSDJ653C5 85237 GB BAI:IECU29579 72221 GBBA1:ECU29579 72221 rxaO11O89 873 GBGSSB:AQ044021 387 E1 3660 Z83859 AL008609 AL021 246 AL021 246 AL034447 L78825 Z99263 AF01 7444 Z99116 Z86099 ACO0251 8 AC00251 8 AC00251 8 AL049743 U29579 U29579 AQ044021 gDNA encoding 6-phosphogluconate dehydrogenase.

Mycobacterium tuberculosis H37Rv complete genome; segment 84/1162.

Mycobacterium leprae cosmid B1788.

Mycobacterium tuberculosis H37Rv complete genome: segment 108/162.

Mycobacterium tuberculosis H37Rv complete genome: segment 108/162.

Sireptomyces coelicolor cosmid WA.

Mycobacterium leprae cosmid B1723 DNA sequence.

Mycobacterium Ieprae cosmid 8637.

Sinorhizobium meliloti NADP-dlependent malic enzyme (tine) gene, complete cds.

Bacillus subtilis complete genome (section 13 of 21): from 2395261 to 2613730.

Herpes simplex virus type 2 (strain HG52), complete genomne.

Homo sapiens chromosome X clone bWXD2O, SEQUENCING IN PROGRESS 11 unordered pieces.

Homo sapiens chromosome X done bWXD2O. SEQUENCING IN PROGRESS I11 unordered pieces.

Homo sapiens chromosome X done bWXD2O, SEQUENCING IN PROGRESS 11 unordered pieces.

Human DNA sequence from dlone 653C5 on chromosome lp 2 l.3- 2 2.3 Contains CA repeat(D15435), STSs and GSSs, complete sequence.

Escherichia coli K-12 genome: approximately 61 to 62 minutes.

Esohenichia coli K-12 genome; approximately 611 to 62 minutes.

CIT-HSP-2318CI 8.TR CIT-HSP Hlomo sapiens genomlc clone 2318C18, genomic survey sequence.

Corynebacterium glutamicumn Mycobacterium tuberculosis Mycobacterium Ieprae Mycobacteriumn tuberculosis Mycobacterium tuberculosis Streptomyces coelicolor Mycobacterium leprae Mycobacteriumn leprae Sinorhizobiumn meliloti Bacillus subtilis human herpesvirus 2 H-omo sapiens Homo sapiens Homo sapiens Homo sapiens Eschenichia coli Escherichia coli Hlomo sapiens 98,349 38,520 64,355 39,860 39,120 55,287 56,847 56,676 53,660 37,255 38,081 35,647 35,647 26,180 36.462 41,808 36,130 36,528 24-Jun-98 17-Jun-98 27-Aug-99 17-Jun-98 17-Jun-98 15-DEC-i1998 15-Jun-96 17-Sep-97 2-Nov-97 26-Nov-97 04-DEC-i 9983 2-Sep-97 2-Sep-97 .2-Sep-97 23-Nov-99 1-Jul-95 1-Jul-95 14-Jul-98 2006200985 24 Feb 2006 GBGSS8:AQ042907 GBGSSB:AQ044021 rxa01093 1554 GBBA1:CORPYKI GBBA1:MTCY0iB2 GB-BA1 :MU65430 rxa0lO99 948 GB-BA2:AF045998 GB-BA2:AFO51 846 GB_GSS1:FR0005503 rxa01l111 541 GBPR3:AC004063 GB,,PR3:HS1 178121 GBHTG3:AC009301 rxa0il3O 687 GBHTG3:AC009444 GBHTG3.AC009444 GBIN1 :DMC66A1 rxa0ll93 1572 GB_BA1:CGASO19 EMPAT:E09634 392 387 2795 35938 1439 780 738 619 177014 62268 163369 164587 164587 34127 1452 1452 36241 1452 AQ042907 AQ044021 127126 Z95554 U65430 AF045998 AF051 846 Z89313 AC004063 AL109852 AC009301 AC009444 AC009444 AL031 227 X76875 E09634 U1 5186 E09634 Table 4 (continued) CIT-HSP-231 8D17.TR CIT-HSP Homo sapiens genomic clone 231 BD1 7, genomic survey sequence.

CIT-HSP-231 8C18.TR CIT-HSP Homo sapiens genomic clone 231BC B.8 genomic survey sequence.

Corynebacterium pyruvate kinase gene, complete cds.

Mycobacterium tuberculosis H37Rv complete genome: segment 72/162.

Mycobacterium intracellulare pyruvate kinase (pykF) gene, complete cds.

Corynebacterium glutamicum inositol monophosphate phosphatase (impA) gene, complete cds.

Corynebactenium glutamicum phosplioribosylformiimino-5-amino-iphosphoribosyl-4- imidazolecarboxamide isomerase (hisA) gene, complete cds.

Frubripes GSS sequence, clone 079Bi16aE8, genomic survey sequence.

Homo sapiens chromosome 4 clone B3218, complete sequence.

Human DNA sequence from clone RP5-1 178121 on chromosome X, complete sequence.

Homo sapiens clone NH0062F14, SEQUENCING IN PROGRESS ~.5 unordered pieces.

Homo sapiens clone 1_0_3, SEQUENCING IN PROGRESS ~,8 unordered pieces.

Homo sapiens clone 1-03, -SEQUENCING IN PROGRESS ~,8 unordered pieces.

Drosophila melanogaster cosmid 66A1.

C.glutamicum (ASO 19) ATPase beta-subunit gene.

Brevibacterium flavumn UncD) gene whose gene product is involved in Mycobacterium leprae cosmid 1471.

Brevibacterium flavumn UncD gene whose gene product is involved in C.glutamnicum (ASO 19) ATPase beta-subunit gene.

Hepatitis C genomic RNA for putative envelope protein (RE45 isolate).

Homo sapiens Homo sapiens Corynebacterium glutamicum Mycobacterium tuberculosis Mycobacterium intracellulare Corynebacterium glutamicum Corynebacterium glutamicum Fugu rubripes Homo sapiens Homo sapiens Homo sapiens Homo sapiens Homo sapiens Drosophila melanogaster Corynebadterium glutamicum Corynebacterium glutamicurn Mycobacterium leprae Corynebacterium giutamicum Corynebacerium glutamicum Hepatitis C virus 35,969 44,545 100,000 63,771 67,061 99,615 100,000 37,785 35,835 37,873 37,240 38,4.16 38,416 38,416 99,931 99,242 39,153 100,000 100,000 36,769 14-Jul-98 14-Jul-98 07-DEC-1994 17-Jun-98 23-DEC-I1996 19-Feb-98 12Z-MAR-1 998 01-MAR-1997 1 0-JuI-98 01-DEC-i1999 1 3-Aug-99 22-Aug-99 22-Aug-99 05-OCT-i1998 27-OCT-I1994 07-OCT-i 997 (Rel. 52, Created) 09-MAAR-1995 07-OCT-1997 (Rel. 52, Created) 27-OCT-1994 5-Apr-92 rxa01194 495 rxaOl200 GBBA1:MLUi5186 EMPAT:E09634 GB_BA1:CGASOi9 GB_VI:HEPCRE4B 1452 X76875 414 X60570 2006200985 24 Feb 2006 rxa0l2Ol 1764 GB-BA1:SLATPSYNA GB-BA1 :MTCY373 GB-BA1:MLU1 5186 rxa0l2O2 1098 GBBAi:SLATPSYNA GBBAi:SLATPSYNA GBBAi:MCSQSSHC rxa01204 933 GBPL1:AP000423 GBHTG6:AC009762 GBHTG6:AC009762 rxa01216 1124 GB-BA1:MTCY1OG2 GBBA2:AF017435

GBBAI:CCRFLBDBA

rxa01225 1563 GB-BA2:AF058302 GB-HTG3AC007301 8560 Z22606 35516 Z73419 36241 U15186 8560 Z22606 8560 Z22606 5538 Y09978 154478 AP000423 164070 AC009762 164070 AC009762 38970 Z92539 4301 AF017435 4424 M69228 25306 AF058302 165741 AC007301 165741 AC007301 Table 4 (continued) Slividans i protein and ATP synthase genes.

Mycobacterium tuberculosis l-37Rv complete genome; segment 57(162.

Mycobacterium leprae cosmid L471.

Slividans i protein and ATP synthase genes.

Slividans i protein and ATP synthase genes.

M.capsulatus orfx, orfy, orfz, sqs and shc genes.

Arabidopsis thaliana chioroplast gerlomic DNA, complete sequence, strain:Columbia.

Homo sapiens clone RPI 1-114116, SEQUENCING IN PROGRESS ~,39 unordered pieces.

Homo sapiens clone RP1 1-114116, *SEQUENCING IN PROGRESS 39 unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 47f162.

Methylobacterium extorquens methanol oxidation genes, glmU-like gene, partial cds, and orfL2, orft.1, orfR genes, complete cds.

C.crescentus fiagellar gene promoter region.

Streptomyces roseof.jlvus frenolicin biosynthetic gene cluster, complete sequence.

Drosophila melanogaster chromosome 2 clone BACRO4BO9 (D576) RPCI-98 04.B.9 map 43E12-44F1 strain y; cn bw sp, -SEQUENCING IN PROGRESS 150 unordered pieces.

Drosophila melanogaster chromosome 2 clone BACRO4BO9 (D576) RPCI-98 0.4.B.9 map 43E12-44F1 strain y; cn low sp, -SEQUENCING IN PROGRESS 150 unordered pieces.

Saccharopolyspora erythraea ferredoxin (fdxA) gene, complete cds.

Mycobacterium tuberculosis H37Rv complete genome, segment 51(1162.

Mycobacterium tuberculosis sequence from clone y348.

Homo sapiens chromosome 17, clone hRPK.138_-P -22, complete sequence.

Homo sapiens clone NH0122109, SEQUENCING IN PROGRESS ~,2 unordered pieces.

Homo sapiens done NH0122L-09, SEQUENCING IN PROGRESS ~,2 unordered pieces.

Streptomyces lividans Mycobacterium tuberculosis Mycobacterium leprae Streptomyces lividans Streptomyces lividans Methylococcus capsulatus Chloroplast Arabidopsis thaliana Homo sapiens Homo sapiens Mycobacterium tuberculosis Methylobacterium extorquens Caulobacter crescentus Streptomyces roseofulvus Drosophila melanogaster 66,269 65,437 39,302 57,087 38,298 37,626 38,395 35,459 36,117 39,064 42,671 41,054 36,205 39,922 01-MAY-i1995 1 7-Jun-95 09-MAR-i 995 01-MAY-1995 01-MAY-i1995 26-MAY-I1998 1 5-Sep-99 04-DEC-i1999 04-DEC-i1999 17-Jun-98 10-MAR-i1998 26-Apr-93 2-Jun-98 17-Aug-99 17-Aug-99 13-MAR-i1996 17-Jun-98 1 0-DEC-i1996 09-OCT-i1998 25-Sep-99 25-Sep-99 rxa0i2l7 444 rxa01242 900 GBHTG3:ACOO73Oi

GB-BAI:SERFOXA

GB-BAI:MSGY348 GBPR3:AC005697 0722 0722 Drosophila melanogaster 39,922 3869 37840 40056 174503 160723 160723 M61119 AL010186 AD000020 AC005697 AC01 0722 AC01 0722 Saccharopolyspora erythraea Mycobacterium tuberculosis Mycobacterium tuberculosis Homo sapiens Homo sapiens Homo sapiens 6.908 62,838 61 .712 35.373 39,863 39,863 2006200985 24 Feb 2006 rxa01243 1083 GB-GSSIO:AQ255057 GBJNI :CEKO5D4 GBJINI:CEKO5D4 rxa01259 9811 GBBA1:CGLPD GBHTG4:AC010567 0567 rxa01262 1284 GBBA2:AF172324 GB_8A2:ECU78086 GB_BA1:D90841 rxa0l3il 870 GB-PR3:AC004103 GB_HTG3:AC007383 GBJITG3:AC007383 nca01312 2142 GB-BA2:AE000487 GBBA1:MTV0i6 GBBA1:U00022 rxa0i32S 795 GBHTG4:AC009245 GB-HTG4:A0009245 GB.-HTG4.AC009245 rxa0i332 576 GBi-ITG6:AC007186 583 19000 19000 1800 143287 143287 14263 4759 20226 144368 215529 215529 13889 53662 36411 215767 215767 215767 225851 AQ255057 Z92804 Z92804 Y16642 AC01 0567 AC01 0567 AF172324 U78086 D90841 AC0041 03 AC007383 AC007383 AE000487 AL021 841 U00022 AC009245 AC009245 AC009245 AC0071 86 Table 4 (continued) mgxbOOO8N0ir CUGI Rice Blast BAC Library Magnaporthe grisea genomic Magnaporthe grisea clone mgxbOO0aN01r genomic survey sequence.

Caenorhabditis elegans cosmid K05D4, complete sequence. Caenorhabditis elegans Caenorhabditis elegans cosmid K05D4, complete sequence, Caenorhabditis elegans Corynebacterium glutamicum lpd gene, complete CDS. Corynebacteriumn glutamicum Drosophila melanogaster chromosome 31J6gC1 clone RPCI98-1 1N6, Drosophila melanogaster SEQUENCING IN PROGRESS 70 unordered pieces.

Drosophila melanogaster chromosome 31J69C1 clone RPC198-1 1IN6, Drosophila melanogaster ~*SEQUENCING IN PROGRESS 70 unordered pieces.

Escherichia col GaIF (gaIF) gene, partial eds; 0-antigen repeat unit transporter Escherichia coi Wzx (wzx), WbnA (wbnA), 0-antigen polymerase Wzy (wzy). WbnB (wbnB), WbnC (wbnC), WbnD (wbnD), WbnE (wbnE), UDP-Glc-4-epimerase GalE (galE), 6-phosphogluconate dlehydrogenase Gnd (gnd), UDP-Glc-6dehydrogenase Ugd (ugd), and WbnF (wbnF) genes, complete cds; and chain length determinant Wzz (wzz) gene, partial cds.

Escherichia coli hypothetical uridine-5'-diphosphoglucose dehydrogenase (ugd) Escherichia coli and 0-chain length regulator (wzz) genes, complete cds.

38,722 35,448 35,694 100,000 37,178 37,178 59.719 23-OCT-i1998 23-Nov-98 23-Nov-98 1 -Feb-99 16-OCT-i1999 1 6-OCT-i 999 29-OCT-1 999 Ecoli genomic DNA, Kohara clone #351(45.1-45.5 min.).

Homo sapiens Xp22 BAC GS-619J3 (Genome Systems Human BAG library) complete sequence.

Homo sapiens clone NHO31O1<15, SEQUENCING IN PROGRESS 4 unordered pieces.

Homo sapiens clone NHO31O11i5, SEQUENCING IN PROGRESS .4 unordered pieces.

Escherichia col K-12 MG1655 section 377 of 400 of the complete genome.

Mycobacterium tuberculosis H37Rv complete genome; segment 143/162.

Mycobacterium leprae cosmid L308.

H-omo sapiens chromosome 7, SEQUENCING IN PROGRESS 24 unordered pieces.

Homo sapiens chromosome 7, SEQUENCING IN PROGRESS ~,24 unordered pieces.

Homo sapiens chromosome 7, SEQUENCING IN PROGRESS **24 unordered pieces.

Drosophila melanogaster chromosome 2 clone BACRO31006 (D569) RPCI-98 03.D.6 map 32A-32A strain y; cn bw sp, **SEQUENCING IN PROGRESS-, 91 unordered pieces.

Drosophila melanogaster chromosome 2 clone BACRi 9N1 8 (0572) RPCI-98 19.N.18 map 32A-32A strain y; cn bw sp, -SEQUENCING IN PROGRESS unordered pieces.

Escherichia coi Homo sapiens Homo sapiens Homo sapiens Escherichia coli Mycobacterium tuberculosis Mycobacterium leprae Homo sapiens Homo sapiens Homo sapiens Drosophila melanogaster 59,735 5-Nov-97 37,904 21-MAR-i 37,340 18-Apr-98 36,385 25-Sep-9 36,385 25-Sep-9t 39,494 12-Nov-91 46,252 23-Jun-99 46,368 01-MAR-i 36,016 2-Nov-99 36,016 2-Nov-99 39,618 2-Nov-99 35.366 07-DEC-i 997 994 999 999 07-DEC-1 GB-HTG6:A0007147 202291 AC007147 Drosophila rnelanogaster 35,366 2006200985 24 Feb 2006 GB-HTG3.AC010207 rxa01350 1107 GB BA2:AF109682 GBHTG2:AC006759 GB-HTG2:AC006759 nca01365 1497 GBBA1:MTY2OB11 GBBAl :XANXANAB 207890 AC010207 rxa01369 1305 rxa01377 1209 rxa01392 1200 GBGSS1O:AQ194038 GBBA1:MTY2OB11 GBGSS3:B10037 GB,,GSS3:B09549 GBBA1:MTCY71 GBHTG5:AC007547 GBHTG5:AC007547 GB-BA2:AF072709 102 103725 36330 3410 697 36330 974 1097 42729 262181 262181 8366 2374 185952 3657 14839 1200 280 349 AF1 09682 AC006759 AC006759 Z95121 M83231 AQ194038 Z95121 B10037 B09549 Z92771 AC007547 AC007547 AF072709 X96471 AC005906 X89084 D90861 E02087 U60627 A1701691 Table 4 (continued) Homo sapiens clone RPCI11-375120, -SEQUENCING IN PROGRESS -,25Homo sapiens unordered pieces.

Aquaspirillum arcticum malate dehydragenase (MDI-) gene, complete cds. Aquaspirillum arcticum Caenarhabditis elegans clone Y4OG1 2, SEQUENCING IN PROGRESS-, Caenorhabditis elegans 8 unordered pieces.

Caenartiabditis elegans clone Y40G12, SEQUENCING IN PROGRESS-, Caenorhabditis elegans 8 unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 139/162. Mycobacterium tuberculosis Xanthomonas campestris phosphoglucamutase and phosphomannomnutase Xanthamonas campestris (xanA) and phasphamannase isomerase and GDP-mannose pyrophospharylase (xanB) genes, complete cds.

RPCI1I 1-47D24.TJ RPCI-1 1 Homo sapiens genomic dlone RPCI-11 1-47D24, Homo sapiens genomic survey sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 139/162. Mycobacterium tuberculosis T27A1 9-17 TAMU Arabidopsis taliana genomic clone T27A11 9, genomic Arabidapsis thaliana survey sequence.

T21A19-T7.1 TAMU Arabidopsisthaliana genomic clone T21A19, genomic Arabidopsis thaliana survey sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 141/162. Mycobacterium tuberculosis Homo sapiens clone RP1 1-252018, WORKING DRAFT SEQUENCE. 121 H-omo sapiens unordered pieces.

H-omo sapiens clone RP1 1-25201 8, WORKING DRAFT SEQUENCE. 121 Homo sapiens unordered pieces.

Streptamyces lividans amplifiable element AUD4: putative Streptomyces lividans transcriptional regulator, putatie ferredoxin, putative cytochrome P450 oxidoreductase, and putative oxidoreductase genes, complete cds; and unknown genes.

C.glutamicum lysE and lysG genes. Corynebacterium glutamicum Homo sapiens l2pl 3.3 BAC RPCI1I 1-429A20 (Roswell Park Cancer Hama sapiens Institute Human BAC Library) complete sequence.

C.glutamicum pta gene and ackA gene. Corynebacterlum glutamicum Ecoll genomic DNA, Kohara clone #405(52.0-52.3 min.). Escherichia coli DNA encoding acetate kinase protein form Escherichia coli. Eschericiia coli Helicobacter pylori feoB-like DNA sequence, genomic survey sequence. Helicobacter pylori we8icO4.xl Soares_-NFL_-T_-GBC_-Si Mama sapiens cDNA clone Hama sapiens IMAGE:2347494 3' similar to gb:119686-rna1 MACROPHAGE MIGRATION INHIBITORY FACTOR (HUMAN);, mRNA sequence.

34,821 58,487 37,963 37,963 38,011 47,726 36,599 36,940 35.284 38,324 39,778 32,658 38,395 55,221 100,000 36,756 100,000 53,041 54,461 39,286 39,412 1 6-Sep-99 19-OCT-i1999 25-Feb-99 25-Feb-99 17-Ju1-.98 26-Apr-93 20-Apr-99 17-Jun-98 14-MAY-1997 14-MAY-i 997 10-Feb-99 16-Nov-99 16-Nov-99 8-Jul-98 24-Feb-97 30-Jan-99 23-MAR-i1999 29-MAY-i1997 29-Sep-97 9-Apr-97 3-Jun-99 GBBA1:CGLYSEG GBPR4:AC005906 rxa01436 1314 GB-BA1:CGPTAACKA GB-BA1 :90861 GB-PAT:E02087 rxa01468 948 GB-GSS1 :HPU60627 GBEST31 :A1701 691 2006200985 24 Feb 2006 Table 4 (continued) GB-EST15:AA480256 389 AA480256 ne3lfO 4.sl NCI_CGAP-Co3 Homo sapiens cDNA clone IMAGE: 898975 3' na01478 1959 rxa01482 1998 GBBA1 :SC 151 GB-BA1 :SCE36 GB-BA1 :CGU43535 GB-BA1 :SC6G4 GBBA1:U00020 GBBAI:MTCY77 40745 '12581 2531 41055 36947 22255 AL109848 AL049763 U43535 AL031317 UJ00020 Z95389 similar to gb:L19686_rnal MACROPHAGE MIGRATION INHIBITORY FACTOR (HUMAN);, mRNA sequence.

Streptomyces coelicolor cosmid 151.

Streptomyces coeticolor cosmid E36.

Corynebacteriumn glutamicumn multidrug resistance protein (cmr) gene, complete cds.

Streptomyces coelicolor cosmid 6G4.

Mycobacterium leprae cosmid B229.

Mycobacterium tuberculosis H37RY complete genome; segment 146/1 62.

Homo sapiens Streptomyces coelicolor A3(2) Streptomyces coelicolor Corynebacteriurn glutamicum Streptomyces coelicolor Mycobacterium leprae Mycobacterium tuberculosis 39,574 14-Aug-97 54,141 38,126 41,852 62,149 38,303 38,179 16-Aug-99 05-MAY-i1999 9-Apr.97 20-Aug-98 01-MAR-1994 1 8-Jun-98 rxa01 534 rxa0lS35 1530 rxa0iS55 1635 GBBA1:MLCB1222 GBBA1:MTVO17 GB.BA1 :PAU72494 GB BA1:090907 GBJIN2:AF0731 77 GBJIN2:AF0731 79 34714 AL049491 67200 AL021 897 4368 U72494 132419 090907 9534 AF073177 3159 AF073179 Mycobacteriumn leprae cosmid B1222. Mycobacteriumn leprae Mycobacterium tuberculosis H37Rv complete genome; segment 48/162. Mycobacterium tuberculosis Pseudomonas aeruginosa fumarase (fumC) and Mn superoxide dismutase Pseudomonas aeruginosa (sodA) genes, complete cds.

Synechocystis sp. PCC6803 complete genome, 9/27, 1056467-1188885. Synechocystis sp.

Drosophila melanogaster glycogen phosphorylase (GlyP) gene, complete cds. Drosophila melanogaster Drosophila melanogaster glycogen phosphorylase (Glpi) mRNA, complete cdls. Drosophila melanogaster 66,208 38,553 52,690 56,487 55,100 56,708 27-Aug-99 24-Jun-99 23-OCT-i1996 7-Feb-99 1-Jul-99 27-Apr-99 rxaol 562 rxa01569 1482 GB SAl :D781 82 GBBA2:AF079139 GB-BA2AF87O22 rxaOlS7O 978 GB-BAI:MTCY63 GB_8A2AF097519 7836 4342 1470 38900 4594 D78182 AF079139 AF087022 Z96800 AF09751 9 Streptccoccus mutans DNA for dTDP-rhamnose synthesis pathway, complete cds.

Streptomyces venezuelae pikCD operon. complete sequence.

Streptomyces venezuelae cytochrome P450 monooxygenase (picK) gene, complete cds.

Mycobacteduum tuberculosis H37Rv complete genome; segment 16/1 62.

Klebsiella pneumoniae dTDP-D-glucose 4.6 dehydratase (rmlB), glucose-i1phosphate thymidylyl Ira nsferase (rmLA), dTDP-4-keto-L-rhamnose reductase (rmlD), dTDP-4-keto-6-eoxy-D-glucose 3,5-epimerase (rmlC), and rhamnosyl transferase (wbbL) genes, complete cds.

Streptococcus mutans Streptomyces venezuelae Streptomnyces venezuelae Mycobacteriurn tuberculosis Klebsiella pneumoniae 44,050 38,587 38,621 59,035 59,714 5-Feb-99 28-OCT-i 998 1 5-OCT-i1998 17-Jun-98 4-Nov-98 2006200985 24 Feb 2006 GBB3A2:NGOCPSPS rxa01l571 723 GBBA1 AB01 1413 GB_-BAi:AB0i 1413 rxa01572 615 GBBA1:AB01 1413 11413 rxa0l6O6 2799 GBVI:CFU72240 GB-GSSIO:AQ21 3248 GBGSS8:AQ070145 rxa01626 468 GB-PR4:AF152510 GBPR4:AF152323 GBPR4:AF152509 rxa0 1632 1128 GBHTG4:AC006590 GB-HTG4:AC006590 GBGSS8:B99182 rxa01633 1206 GBBA1:BSUB0009 GBBA1:BSUBOOO9 GB-ITG2:.AC006247 Table 4 (continued) 8905 109189 Neissenia meningitidis dTD)P-D-glucose 4.6-dehydratase (rfbB), glucose-i Neisseria meningitidis phosphate thymidyl transferase (rfbA) and rfbC genes, complete cds and UPDglucose-4-epimerase (galE) pseudogene.

12070 AB0'11413 Streptomyces griseus genes for Orf2, Orf3, Orf4. Orf5, AfsA, Orf8, partial and Streptomyces griseus complete cds.

12070 ABO11413 Streptomyces griseus genes for Orf2, Orf3, Orf4, Orf5, AfsA, Orf8, partial and Streptomyces griseus complete cds.

12070 AB011413 Streptomyces gniseus genes for Orf2, Orf3, Orf4, Orf5, AfsA, Orf8, partial and Streptomyces griseus complete cds.

12070 ABO11413 Streptomyces griseus genes for Orf2, Orf3, Orf. 06f5, AfsA. Orf8, partial and Streptomyces griseus complete cds.

4783 U72240 Choristoneura fumniferana nuclear polyhedrosis virus ETMV protein liomolog, 79 Choristoneura fumiferana kDa protein homolog. 15 kDa protein homolog and GTA protein homolog nucleopolyhedrovirus genes, complete cds.

408 AQ21 3248 HS,.3249_81_A02_MR CIT Approved Human Genomic Sperm Library D Homo Homo sapiens sapiens genomic clone Plate=3249 CoI=3 Row--B. genomic survey sequence.

285 AQ070145 H- 3027_BiH02_MR CIT Approved Human Genomic Sperm Library D Homo Homo sapiens sapiens genomic clone Plate=3027 Col=3 Row=P, genomic survey sequence.

2490 AF152510 Homo sapiens protocadherin gamma A3 short form protein (PCDH-gamma-A3) Homo sapiens variable region sequence, complete cds.

4605 AF152323 H-omo sapiens protocadhedn gamma A3 (PCDH-gamma-A3) mRNA, complete Homo sapiens cds.

2712 AF152509 Homo sapiens PCDH-gamma-A3 gene, aberrantly spliced, mRNA sequence. Homo sapiens 127171 AC006590 Drosophila melanogaster chromosome 2 clone BACR1 3NO2 (D543) RPCI-98 Drosophila melanogaster 13.N.2 map 36E-36E strain y: cn bw sp, SEQUENCING IN PROGRESS-, 101 unordered pieces.

127171 AC006590 Drosophila melanogaster chromosome 2 clone BACR13NO2 (D543) RPCI-98 Drosophila melanogaster 13.N.2 map 36E-36E strain y; 01 bw sp, SEQUENCING IN PROGRESS-, 101 unordered pieces.

415 899182 CIT-HSP-2280113.TR CIT-HSP Homo sapiens genomic clone 2280113. Homo sapiens genomic survey sequence.

208780 Z991 12 Bacillus subtilis complete genome (section 9 of 21): from 1598421 to 1807200. Bacillus subtilis 208780 Z99112 Bacillus subtilis complete genome (section 9 of 21): from 15981421 to 1807200. Bacillus subtilis 174368 AC006247 Drosophila melanogaster chromosome 2 clone BACR48110 (D505) RPCI-98 Drosophila melanogaster 48.1.10 map 49E6-49F8 strain y: cen bw sp, **SEQUENCING IN PROGRESS -,17 unordered pieces.

34,559 18-Sep-98 40,351 5-Aug-98 CD 58,384 57,500 35,655 57,843 38,119 37,115 30-Jul-96 7-Aug-98 7-Aug-98 7-Aug-98 7-Aug-98 29-Jan-99 34,.298 34,298 34,298 33,812 33,812 36,111 36,591 34,941 37,037 14-Jul-99 22-Jul-99 14-Jul-99 19-OCT-i1999 19-OCT-i1999 26-Jun-98 26-Nov-97 26-Nov-97 2-Aug-99 2006200985 24 Feb 2006 rxa01695 1623 GB-BA1:CGA224946 GBBAl :MTCY24A1 GBIN1:DMU15974 rxaOl7O2 1155 GB-BAI:CGFDA GBBA1:MTY13E10 GBBA1:MLCB4 rxaD1743 901 GB_1N2:CELC27H5 GB-EST24:A1671 12 GBGSS9.AQ102635 rxa01744 1662 GB-BA1:MTCYO1B2 GBGSS1:AF009226 GBBA1:SCD78 rxa01745 836 GBBA1:MTCY19O GBBA1:MLCB22 GB-BA2:AE0OO1 75 nca01758 1140 GBPR3:HS57G9 GBPL2:YSCH9666 GB,.PL2:YSCH9986 rxa01814 1785 GBBA1:ABCCELB GB-BA1 :MTCY22D7 GBBA1:MTCY22D7 1809 GBGSS9:A0142579 GB-jN2:AC005889 GBGSSI AGG08814 2408 20270 2994 3371 35019 36310 35840 579 347 35938 665 36224 34150 40281 15067 113872 39057 41664 2058 31859 31859 529 108924 637 AJ224946 Z95207 U15974 X17313 Z95324 AL02351 4 U14635 A1167112 AQ102635 Z95554 AF009226 AL034355 Z70283 Z98741 AE000175 Z95116 U 10397 U00027 L24077 Z83866 Z83866 AQ142579 AC005889 AGO0881 4 Table 4 (continued) Corynebacterium glutamicum DNA for L-Malate:qui none oxidoreductase.

Mycobacterium tuberculosis H37Rv complete genome; segment 1241162.

Drosophila melanogaster kinesin-like protein (klp68d) mRNA, complete cds.

Corynebacterium glutamicum fda gene for fructose-bisphosphate aldolase (EC 4.1.2.13).

Mycobacterium tuberculosis H37Rv complete genome; segment 18/162.

Mycobacterium leprae cosmid B4.

Caenorhabditis elegans cosmid C271-5.

xylem.est.878 Poplar xylem Lambda ZAPII library Populus balsamifera subsp.

tricliocarpa 6DNA mRNA sequence.

HS 7 3048B-F08-MF CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3048 Col=1 5 Row=L, genomic survey sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 72/162.

Mycobacterium tuberculosis cytochrome D oxidase subunit I (appC) gene, partial sequence, genomic survey sequence.

Streptomyces coelicolor cosmid 078.

Mycobacterium tuberculosis H37Rv complete genome; segment 98/162.

Mycobactenumr leprae cosmid B22.

Escherichia coi K-12 MG1 655 section 65 of 400 of the complete genome.

Human DNA sequence from BAC 57G9 on chromosome 22q12.1 Contains ESTs, CA repeat, GSS.

Saccharomyces cerevisiae chromosome ViII cosmid 9666.

Saccharomyces cerevisiae chromosome ViII cosmid 9986.

Acetobacter xylinum phosphoglucomutase (ceIB) gene, complete cds.

Mycobacterium tuberculosis H37Rv complete genome; segment 133/1 62.

Mycobacterium tuberculosis H37Rv complete genome; segment 133/162.

Corynebacterium glutamicumn Mycobacteriumn tuberculosis Drosophila melanogaster Corynebactenium glutamicum Mycobacterium tuberculosis Mycobacteriumn leprae Caenorhabditis elegans Populus balsamifera subsp. trichocarpa Homo sapiens Mycobacterium tuberculosis Mycobacternum tuberculosis Streptomyces, coelicolor Mycobacterium tuberculosis Mycobacterium leprac Escherichia coli Homo sapiens 100.000 38,626 36,783 99,913 38,786 38,238 35,334 39,222 40,653 36,650 63,438 53.088 62,081 61,364 52,323 39,209 11-Aug-98 17-Jun-98 18-Jul-95 1 2-Sep-93 17-Jun-98 27-Aug-99 13-Jul-95 03-DEC-1998 27-Aug-98 17-Jun-98 31-Jul-97 26-Nov-98 17-Jun-98 22-Aug-97 12-Nov-98 23-Nov-99 5-Sep-97 29-Aug-97 21-Sep.94 17-Jun-98 17-Jun-98 Saccharomyces cerevisiae 40,021 Saccharomyces cerevisiae 3.4,375 Acetobacter xylinus 62,173 Mycobacterium 39,749 tuberculosis Mycobactenumr 40,034 tuberculosis HS_72222_Bi_1103_MR CIT Approved Human Genomic Sperm Library 0 Homo Homo sapiens sapiens genomic clone Plate=2222 001=5 Row--P, genomic survey sequence.

Drosophila melanogaster, chromosome 2L, region 30A3- 30A6, P1 clones Drosophila melanogaster DS06958 and DS03097, complete sequence.

Homo sapiens genomic DNA, 21q region, clone: B1137B858, genomic survey Homo sapiens sequence.

38,068 24-Sep-98 36,557 35,316 30-OCT-1998 7-Feb-99 2006200985 24 Feb 2006 rxa01859 1050 GB-BA2:AF183408 GBJITG5:AC008031 GBBA2:AF183408 nxa01865 438 GBBAI:SERFDXA GBBA1:MTVOOS GB.,BA1 :MSGY348 rxa01882 1113 GB-PR1-IUMADRA2C GBPR4:HSU72648 GBGSS3:B42200 rxa01884 1913 GBBA1:MTCY48 GB-BA1:SCOOO1 206 GBBA1:D90908 rxa018B6 897 GB-GSS9:AQ1 16291 GB BA2:AE001721 GBEST16:PA567090 rxa01887 1134 GBHTGG:AC008147 GBHTG6:AC008147 GBBA2:ALW243431 63626 AF1 83408 158889 63626 3869 37840 40056 1491 4850 387 35377 9184 122349 572 17632 596 303147 303147 26953 AC008031 AF183408 M61119 AL0 10186 AD000020 J03853 U72648 B42200 Z74020 AJO01206 D90908 A01 16291 AE001721 AA56709D AC008147 AC008147 AJ243431 Table 4 1continued) Microcystis aeruginosa DNA polymerase III beta subunit (dnaN) gene, partial Mic-rocystis aeruginosa cds: microcystin synthetase gene cluster, complete sequence; Umal (umal).

Umna2 (uma2). Uma3 (uma3), Uma4 (uma4), and Uma5 (uma5) genes, complete cds: and Uma6 (uma6) gene, partial cds.

Trypanosoma brucei chromosome 11 clone RPC193-25N14, SEQUENCING Trypanosoma brucel IN PROGRESS 2 unordered pieces.

Microcystis aeruginosa DNA polymerase III beta subunit (dnaN) gene, partial Microcystis aeruginosa cds; microcystin synthetase gene cluster, complete sequence; Umal (umal), Uma2 (uma2), Uma3 (uma3), Uma4 (uma4), and Uma5 (uma5) genes, complete cds; and Uma6 (uma6) gene, partial cds.

Saccharopotyspora erythraea ferredoxin (fdxA) gene, complete cds, Saccharopolyspora erythraea Mycobacterium tuberculosis H37Rv complete genome; segment 51f162. Mycobacterium tuberculosis Mycobacterium tuberculosis sequence from clone y348. Mycobacterium tuberculosis Human kidney alpha-2-adrenergic receptor mRNA. complete cds. Homo sapiens Homo sapiens alpha2-C4-adrenergic receptor gene, complete cdls. Homo sapiens HS-1055-B31-A03-MR.abi CIT Human Genomic Sperm Library C Homo sapiens Homo sapiens genomic clone Plate=CT 777 CoI=5 Row--B, genomic survey sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 691162. Mycobacterium tuberculosis Streptomyces coelicolor A3(2), glycogen metabolism cluster 11. Streptomyces coelicolor Synecliocystis sp. PCC6803 complete genome, 10127, 1188886-1311234. Synechocystis sp.

RPCI1I1-49P6.TK.1 RPCI-11 IHomo sapiens genomic clone RPCI-11I-49P6, Homo sapiens genomic survey sequence.

Thermotoga maritima section 33 of 136 of the complete genome. Thermotoga maritima GM01044.5prime GM Drosophila melanogaster ovary BlueScript Drosophila Drosophila melanogaster melanogaster cDNA clone GM01044 5prime, mRNA sequence.

Homo sapiens clone RP3-405J110, SEQUENCING IN PROGRESS ~,102 Homo sapiens unordered pieces.

Homo sapiens clone RP3-A05J10, SEQUENCING IN PROGRESS ~,102 Homo sapiens unordered pieces.

Acinetobacter Iwoffli wzc, wzb, wza, weeA, weeB, wceC, wzx, wzy, weeD, Acinelobacter twoflii weeE, weeF, weeG, weeH, weel, weeJ, weeK, galU, ugd, pgi, galE, pgm (partial) and mip (partial) genes (emulsan biosynthetic gene cluster), strain

RAG-I.

36.364 35,334 36,529 59,862 61,949 59,908 36,899 36,899 34,805 37.892 40,413 47,792 43,231 39,306 42,807 36,417 37,667 39,640 03-OCT- 1999 1 5-Nov-99 03-OCT-1999 13-MAR-1996 17-Jun-98 10-DEC-i1996 27-Apr-93 23-Nov-98 18-OCT-1997 17-Jun-98 29-MAR-i1999 7-Feb-99 20-Apr-99 2- -Jun-99 28-Nov-98 03-DEC-i 999' 03-DEC-1 999 01-OCT-i1999 rxa01888 658 GBHTG2.AC008197 125235 ACCO8197 Drosophila melanogaster chromosome 3 clone BACRO2L-12 (D753) RPCI-98 Drosophila melanogaster 32,969 2-Aug-99 021.12 map 94B-94C strain y; cen bw sp, -SEQUENCING IN PROGRESS-, 113 unordered pieces.

2006200985 24 Feb 2006 GBHTG2:AC008197 GBE5T36:A1881527 rxa01891 887 GB-VI:H1V232971 GBPL1:AFCHSE GBPR3:AF064858 nxa01895 1051 GBBA1:CGL238250 GBBA2:AF038423 GBBA1 :MTCY3S9 rxaOIS0l 1383 GBBA1:MSGB3BCOS GB-BA1 :SCE63 GB-PR3:AF0931 17 rxa01927 1503 GB8BA1:CGPAN GB,.BA1:ASXYLA GBHTG3:AC009500 rxa01952 1836 GBBA2:AE000739 G8-EST28:AI5i 9629 GBEST21:AA949396 rxa01989 630 GB SAl :BSPGIA GB-BA1 :BSUBO0i7 GB-BA2:AF1 32127 rxa02O26 720 GBBA1:SXSCRBA GBBA1:BSUBOO2O GB-BA1 :BSGENR rxa02O28 526 GB-BA1 :MTC1237 125235 AC008197 598 621 6158 193387 1593 1376 36021 37114 37200 147216 2164 1905 176060 13335 612 767 1822 217420 8452 3161 212150 97015 27030 A1881527 AJ232971 Y09542 AF064858 AJ238250 AF038423 Z83859 L01095 AL035640 AF0931 17 X96580 X59466 AC009500 AE000739 A151 9629 AA949396 X16639 Z99120 AF1 32127 X67744 Z99123 X73124 Z94752 Table 4 (continuedg) Drosophila melanogaster chromosome 3 clone BACR02LI2 (D753) RPCI-98 Drosophila melanogaster 32.969 021.12 map 9413-94C strain y; cn bw sp, SEQUENCING IN PROGRESS 113 unordered pieces.

606070C09.yl 606 Ear tissue cONA library from Schmidt lab Zea mays cDNA, Zea mays 43,617 mRNA sequence.

Human immunodeficiency virus type 1 subtype C nef gene, patient MP83. Human immunodeficiency 40,040 virus type 1 A.fumigatus chsE gene. Aspergillus fumnigatus 37,844 Homo sapiens chromosome 21q22.3 BAC: 28179. complete sequence. Homo sapiens 37,136 Corynebacterium glutamicumn ndh gene. Corynebacterium, 100,000 glutamicumn Mycobacterium smegmatis NACH dehydrogenase (ndh) gene, complete cds. Mycobacteriumn smegmatis 65,254 Mycobacterium tuberculosis l137Rv complete genome; segment 84/1162. Mycobacterlum 40,058 tuberculosis M. leprae genomic DNA sequence, cosmid 838 btr gene, complete cds. Mycobacteriumn Ieprae 59,551 Streptomyces coelicolor cosmid E63. Streptomyces coelicolor 39,468 Homo sapiens chromosome 7qtelo SAC E3. complete sequence. Homo sapiens 39,291 C.glutamicumn panB, panC xylB genes. Corynebacteriumn 38,384 glutamicumn Arthrobacter Sp. N.R.R.L. B3728 xylA gene for D-xylose(D-glucose) isomerase. Arthrobacter sp. 56,283 Homo sapiens clone NHO511A2O, SEQUENCING IN PROGRESS 6 Homo sapiens 37,593 unordered pieces.

Aquifex aeolicus section 71 of 109 of the complete genome. Aquifex aeolicus 36,309 LD39282.5prime LD Drosophila melanogaster embryo pOT2 Drosophila Drosophila melanogaster 41,941 melanogaster cDNA clone LD39282 5prime, mRNA sequence.

LD28277.5prime LD Drosophila melanogaster embryo pOT2 Drosophila Drosophila melanogaster 39,855 melanog aster cDNA clone LD28277 5prime, mRNA sequence.

Bacillus stearothermophilus pgiA gene for phosphoglucoisomnerase isoenzyme Bacillus 66,292 A (EC stearothermophilus Bacillus subtilis complete genome (section 17 of 21): from 3197001 to Bacillus subtilis 37,255 3414420.

Streptococcus mutans sorbitol phosphoenolpyruvate:sugar phosphotransferase Streptococcus mutans 63,607 operon, complete sequence and unknown gene.

S.xylosus scrB and scrR genes. Staphylococcus xylosus 67,778 Bacillus subtilis complete genome (section 20 of 21): from 3798.401 to Bacillus subtilis 35,574 4010550.

S.subtilis genomic region (325 to 333). Bacillus subtilis 51,826 Mycobacterium tuberculosis H37Rv complete genome; segment 46/162. Mycobactenumr 54,476 tuberculosis 2-Aug-99 21-Jul-99 05-MAR-1 999 1 -Apr-97 2-Jun-98 24-Apr-99 05-MAY-i1998 17-Jun-98 6-Sep-94 17-MAR-i1999 02-OCT-i1998 11 -MAY- 1999 04-MAY-i1992 24-Aug-99 25-MAR-i1998 16-MAR-i1999 25-Nov-98 20-Apr-95 26-Nov-97 28-Sep-99 28-Nov-96 26-Nov-97 2-Nov-93 17-Jun-98 2006200985 24 Feb 2006 rxa02054 1140 rxa02056 2891 rxaO2O6l 1617 rxa02063 1350 rxaO2lOO 2348 rxa02122 822 rxaO214O 1200 fxa02142 774 GBPL2-:SCE9537 GB-GSS13:AQ5O1 177 GB BA1:MLCB 1222 GBBA1:MTYi3EI2 GBBAl :MTU43540 GB-PAT:E14601 GBBA1:D84102 GBBA1:MTVOO6 GB-TG7:AC005883 GB PL2:ATACOO3O33 GBPL2:ATAC002334 GBBA1:SCGLGC GB-GSS4:AQ687350 GBEST38:AW028530 GB-BA1 :MSGY151 GB-BA1:MTCY130 GBBAl :SCOOO 1205 GBBA1:Dg0858 GBEST37:A1948595 GB..HTG3AC0iO387 66030 767 34714 43401 3453 4394 4394 22"0 211682 84254 75050 1518 786 444 37036 32514 9589 13548 469 220665 U18778 AQ501 177 AL049491 Z95390 U43540 E14601 D84102 AL021 006 AC005883 AC003033 AC002334 X89733 A0687350 AW028530 AD00001 8 Z73902 AJO01205 D90858 A1948595 AC01 0387 L78813 L78814 AF093099 Z70283 genomic survey sequence.

Mycobacterium Ieprae cosmid 81222.

Table 4 (continued) Saccharomyces cerevisiae chromosome V cosmids 9537, 9581, 9495, 9867, Saccharomyces cerevisiae 36,100 and lambda clone 5898.

V26G9 mTn-3xHAIlacZ Insertion Library Saccharomyces cerevisiae genomic Sacctiaromyces cerevisiae 32,039 Mycobacterium leprae Mycobacterium tuberculosis H-37Rv complete genome; segment 1471162. Mycobacterium tuberculosis Mycobacterium tuberculosis rfbA, rhamnose biosynthesis protein (rfbA), and Mycobacterium rmlC genes, complete cds. tuberculosis Brevibacterium lactofermentum gene for alpha-ketoglutaric acid Corynebactenium dehydrogenase. glutamicum Corynebacterium glutamicum DNA for 2-oxoglutarate dehydrogenase. complete Corynebacterium cds. glutamicum Mycobacterium tuberculosis H37Rv complete genome; segment 54/162. Mycobacterium tuberculosis Homo sapiens chromosome 17 clone RP1 1-958E1 1 map 17, Homo sapiens SEQUENCING IN PROGRESS 2 ordered pieces.

Arabidopsis thaliana chromosome 11 BAC T211.14 genomic sequence, complete Arabidopsis thaliana sequence.

Arabidopsis thaliana chromosome 11 BAC F25118 genomic sequence, complete Arabidopsis thaliana sequence.

S.coelicolor DNA for glgC gene. Streptomyces coelicolor nbxbOO74H1 1 r CUGI Rice BAC Library Oryza sativa genomic clone Oryza sativa nbxbOO74Hll1r, genomic survey sequence.

wv27fl O.xl NCL CGAP Kidi 11 Homo sapiens cDNA clone IMAGE:2530795 3' Homo sapiens similar to WP:TO3G1 1.6 CE04874 mRNA sequence.

Mycobacterium tuberculosis sequence from clone y151. Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genome: segment 59/162. Mycobacterium tuberculosis Streplomyces coelicolor A3(2) glycogen metabolism cluster. Streptomyces coelicolor E.coli genomic DNA, Kohara clone #401(51.3-51.6 min.). Escherichia coli wqO7dl2.xl NCICGAPKid12 Hlomo sapiens cDNA clone IMAGE:2470583 llama sapiens mRNA sequence.

llama sapiens chromosome 5 clone CITB-H1 207408, -SEQUENCING IN Homo sapiens PROGRESS 77 unordered pieces.

Mycobacterium leprae cosmid B1551 DNA sequence. Mycobacterium leprae Mycobacterium leprae cosmid B1554 DNA sequence. Mycobacterium leprae Mus musculus transcription factor TBLYM (Tblym) mRNA, complete cds. Mus musculus Mycobacterium tuberculosis H37RY complete genome; segment 98/162. Mycobacterium tuberculosis 61,896 59,964 59,659 98,928 98,928 39,265 37,453 37,711 37,711 56,972 40,696 36,795 40,156 55,218 38,475 38,586 37,259 38,868 51,399 51,399 36,683 57,292 1 -Aug-97 29-Apr-99 27-Aug-99 17-Jun-98 14-Aug.97 28-Jul-99 6-Feb-99 1 8-Jun-98 08-DEC-i1999 19-DEC-i1997 04-MAR-i1998 12-Jul-99 1-Jul-99 27-OCT-i1999 10-DEC-i1996 17-Jun-98 29-MAR-i1999 29-MAY-i1997 6-Sep-99 15-Sep-99 15-Jun-96 15-Jun-96 01-OCT-1999 17-Jun-98 GB-BA1:MSGB1551CS 36548 GB BA1:MSGBI554CS 36548 GB ROAF093099 2482 GB,,BA1:MTCY190 34150 2006200985 24 Feb 2006 GB..BA1 :SC6G1O 36734 AL049497 GBBA1:AB016787 5550 nca02143 1011 GBBA1:MTCY190 34150 GB BA1:MSGB1 551 CS 36548 GBSAl :MSGB1554CS 36548 rxa02144 1347 GBBAI:MTCY190 34150 GBHTG3:ACO1 1 5000 300851 GBJ-HTG3:AC01 1 5000 300851 nca02147 1140 GBEST28:A1492095 GBEST1O:MA157467 GB-EST1 0:AA1 57467 rxa02149 1092 GB-PR3:HSBK277P6 GBBA2:EMB065R075 GB-EST34:A1789323 rxa02175 1416 GB-BA1:CGGLTG GBBA1:MTCY31 GBBA1:MLCB57 rxa02196 816 GBO:RATDAPRP GB-GSS:AQ012162

GBRO:RATDAPRP

rxa02209 1694 GBBA1 :AB025424 GB.BA2AF002133 485 376 376 61698 360 574 3013 37630 38029 2819 763 2819 2995 15437 ABO 16787 Z70283 L78813 L78814 Z70283 AC0l 1500 AC01 1500 A1492095 AA157467 AA1 57467 ALl 17347 AFi 16423 A1789323 X66112 Z731 01 Z99494 M76426 A0012162 M76426 AB025424 AF0021 33 Table 4 (continued) Streptomyces coelicolor cosmid 6G1 0.

Pseudomonas putida genes for cytochrome o ubiquinol oxidase A-E and 2 ORFs, complete cds.

Mycobacterium tuberculosis H37Rv complete genome; segment 98/162.

Mycobacteriumn leprae cosmid BI 551 DNA sequence.

Mycobacterium leprae cosmid B1554 DNA sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 98/162.

Homo sapiens chromosome 19 clone CIT978SKB-60E1 1, SEQUENCING IN PROGRESS 246 unordered pieces.

Homo sapiens chromosome 19 clone CIT978SKB-60E1 1, SEQUENCING IN PROGRESS 246 unordered pieces.

tgO7a0l x1 NCI$CGAPOLLi Homo sapiens cONA clone IMAGE:2108040 3', mRNA sequence.

zoS0e01.rl Stratagene endothelial cell 937223 Homo sapiens cDNA clone IMAGE:590328 mRNA sequence.

zoS0e0l .rl Stratagene endothelial cell 937223 Homo sapiens cONA clone IMAGE:590328 mRNA sequence.

Human DNA sequence from clone 277P6 on chromosome lq25.3-31 .2, complete sequence.

Rhizobium etli mutant MB045 RosR-tra nscription ally regulated sequence.

uk53g05.yl Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE: 1972760 5' similar to WP:K1 1 Hi12.8 CEl 2160:. mRNA sequence.

C.glutamicumn git gene for citrate synthase and ORIF.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 41/1 62.

Mycobacteriumn Ieprae cosmid B57.

Rattus norvegicus dipeptidyl aminopeptidase-related protein (dpp6) mRNA, complete cds.

127PB037070197 Cosmid library of chromosome 11 Rhodobacter sphaeroides genomic done 127PB037070197, genomic survey sequence.

Rattus norvegicus dipeptidyl aminopeptidase-related protein (dpp6) mRNA, complete cds.

Corynebacteriumn glutamicum gene for aconitase. partial cds.

Mycobacteriumn avium strain GIR10 transcriptional regulator (mnay81) gene, partial cds. aconitase (acn), invasin 1 (invi), invasin 2 (inv2), transcriptional regulator (moxR), ketoacyl-reductase (fabG), enoyl-reductase (inhA) and ferrochelatase (mav272) genes, complete cds.

Streptomyces coelicolor Pseudomonas putida Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium leprae Mycobacterium tuberculosis Homo sapiens Homo sapiens Homo sapiens Homo sapiens Homo sapiens H-omo sapiens Rhizobium etli Mus musculus Corynebacterium glulamicum Mycobacteriurn tuberculosis Mycobacterium leprae Rattus norvegicus Rhodobacter sphaeroides Rattus norvegicus Corynebacterium glutamicum Mycobactenum avium 35,058 47,403 57,317 38,159 38,159 55,530 39,659 39,659 39,798 36,436 36.436 36,872 43,175 39,715 100,000 64,331 62,491 38,791 40,044 37,312 99,173 40,219 24-MAR-i1999 5-Aug-99 17-Jun-98 1 5-Jun-96 15-Jun-96 17-Jun-98 1 8-Feb-00 1 8-Feb-00 30-MAR-1999 1 1-DEC-1996 1 1-DEC-1996 23-Nov-99 06-DEC-i1999 2-Jul-99 17-Feb-95 17-Jun-98 1 0-Feb-99 31-MAY-1995 4-Jun-98 31-MAY-i1995 3-Apr-99 26-MAR-i1998 2006200985 24 Feb 2006 rxaO2213 874 GB-BA1:MTVOO7 GBBAlAB025424 GB-BA1:MTVOO7 GB-BA2:AFOO2l 33 32806 2995 32806 15437 AL021 1184 AB025424 AL0211 B4 AF0021 33 Table 4 (continued) Mycobacteriumn tuberculosis H37Rv complete genome; segment 641162. Mycobacterium tuberculosis Corynebacterium glutamicum gene for aconitase, partial cds. Corynebacterium glutamicum Mycobacterium tuberculosis H37Rv complete genome; segment 64/162. Mycobacteriumn tuberculosis Mycobacterium avium str ain GIRlO0 transcriptional regulator (may8 1) gene, Mycobacterium avium partial cds, aconitase (acn), invasin 1 (invi). invasin 2 (inv2), transcriptional regulator (moxR), ketoacyl-reductase (fabG), enoyl-reductase (inhA) and ferrochelatase (mav272) genes, complete cds.

Rhodobacter capsulatus; Calvin cycle carbon dioxide fixation operon: fructose- Rhodlobacter capsulatus 1,6-lsedoheptulose-1.7-bisphosphate aldolase (cbbA) gene, partial cds, Form 11 ribulose-l 5-bisphosphate carboxylase/oxygenase (cbbM) gene, complete cds, and Calvin cycle operon: pentose-5-phosphate-3-epimerase (cbbE), phosphoglycolate phosphatase (cbbZ), and cbbY genes, complete ods.

38,253 99.098 34,937 36,885 48,701 17-Jun-98 3-Apr-99 17-Jun-98 26-MAR-1 998 28-OCT-1 997 rxa02245 780 GB-BA2:RCU23145 5960 U23145 rxa02256 1125 rxa02257 1338 rxa02258 900 rxa02259 2895 GBBA1 :ECU82664 GBHTG2:AC007922 GBBAl :CGGAPPGK GB BAI :SCC54 GBBA1:MTCY493 GBBA1:CGGAPPGK GBBA1:MTCY493 GBBA2:MAU82749 GBBAl :CGGAPPGK GB-BA1 :CORPEPC GBYPAT:A09073 GB-BA1 :CORPEPC: GB-PATA09073 GB-BA1 :CGPPC 139818 U82664 158858 AC007922 3804 X59403 30753 AL035591 40790 Z95844 3804 X59403 40790 Z95844 2530 U82749 3804 X59403 4885 M25819 4885 A09073 4885 M25819 4885 A09073 3292 X14234 Escherichia c0li minutes 9 to 11 genomic sequence.

Homo sapiens chromosome 18 done hRPK. 178_-F710 map 18, SEQUENCING IN PROGRESS 11 unordered pieces.

C.glutamicum gap, pgk and tpi genes for glyce ralIdehyde-3-p hosph ate, phosphoglycerate kinase and triosephosphate isomerase.

Streptomyces coelicolor cosmid C54- Mycobacterium tuberculosis H37Rv complete genome; segment 63/162.

C.glutamicumn gap, pgk and tpi genes for glyceraldlehyde-3-phos phate, phosphoglycerate kinase and tniosephosphate isomerase.

Mycobacterium tuberculosis H-37Rv complete genome; segment 63/162.

Mycobacterium avium glyceraidehyde-3-phosphate dlehydrogenase homolog (gapdh) gene, complete cds; and phosphoglycerate kinase gene, partial cds.

C.glutamicum gap, pgk and tpi genes for glyceraldehyde-3-phosphate, phosphoglycerate kinase and triosephosphate isomerase.

C.glutamicum phosphoenolpyruvate carboxylase gene, complete cds.

C.glutamicum ppg gene for phosphoenol pyruvate carboxylase.

C.glutamicumn phosphoenolpyruvate carboxylase gene, complete cds.

C.glutamicumn ppg gene for phosphoenol pyruvate carboxylase.

Corynebacterium glutamicum phosphoenolpyruvate carboxylase gene (EC 4.1.1.31).

Escherichia coli Homo sapiens Corynebacteriumn glutamicumn Streptomyces coelicolor Mycobacteriumn tuberculosis Corynebactenium glutamicum Mycobacterium tuberculosis Mycobacterium aviumn Corynebacterium glutamicumn Coryinebacterium glutamicum Corynebacteriumn glutamicum Corynebacterium glutamicum Corynebacteriurn glutamicum Corynebacterium glutamicumn 33,118 99,289 36,951 64.196 98,873 61,273 61,772 99,667 100,000 100,000 100,000 100,000 99,827 26-Jun-99 05-OCT-i1992 11-Jun-99 19-Jun-98 05-OCT-i1992 19-Jun-98 6-Jan-98 05-OCT-i1992 1 5-DEC-1 995 25-Aug-93 15-DEC-1995 25-Aug-93 12-Sep-93 39,119 11-Jan-97 2006200985 24 Feb 2006 rxa02288 969 GBPR3:l-SDJ94E24 GBIITG3:ACO1 0091 GBHTG3:AC010091 rxa02292 798 GBBA2:AF125164 GB.GSS5AQ744695 GB-EST1 4AA381 925 rxa02322 511 GB-BA1 :MTCY22G8 GB-BA1 :MTCY22G8 rxa02326 939 rxa02327 1083 rxa02328 1719 rxa02332 1266 rxa02333 1038 GB_BA1:CGPYC GBBA2:AF038548 GB-BA1 :MTCY349 GBBA1:CGPYC GB-BA2:AF038548 GBBA1:MTCY349 GBBA1 :CGPYC GBBA2AF038548 GB-PL2:AF097728 GB-BA1 :MSGLTA GBBA2:ABU85944 GB-BA2:AE0OO1 75 GB-BA1 :MSGLTA 243145 AL050317 159526 AC010091 159526 ACO 10091 26443 AF125164 827 A0744695 309 AA381925 22550 Z95585 22550 Z95585 3728 Y09548 3637 AF038548 43523 Z83018 3728 Y09548 3637 AF038548 43523 Z83018 3728 Y09548 3637 AF038548 3916 AF097728 1776 X60513 1334 U85944 15067 AE000175 1776 X60513 Table 4 (continued) H uman DNA seq uence from clone RP 1-94 E24 on chromosome 20q 12.

complete sequence.

Homo sapiens clone NH0295A01, SEQUENCING IN PROGRESS ~.4 unordered pieces.

Homo sapiens clone NH0295AO1, SEQUENCING IN PROGRESS ~,4 unordered pieces.

Bacteroides fragilis 638R polysaccharide B (PS B32) biosynthesis locus, complete sequence; and unknown genes.

HS_5505_A2_C06_SP6 RPCI-1 1 Human Male BAC Library Homo sapiens genomic clone Plate=1081 Col=12 Row--E. genomic survey sequence.

EST95058 Activated T-cells I Homo sapiens cDNA V end, mRNA sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 49/162.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 49/162.

Corynebacterium glutamicum pyc gene.

Corynebacterium glutamicum pyruvate carboxylase (pyc) gene, complete cds.

Mycabacteriumn tuberculosis H37Rv complete genome; segment 131/162.

Corynebacterium glutamicum pyc gene.

Corynebacterium glutamicum pyruvate carboxylase (pyc) gene, complete cds.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 131/1 62.

Corynebacterium glutamicum pyc gene- Corynebacterium glutamicum pyruvate carboxylase (pyc) gene, complete cds.

Aspergillus terreus pyruvate carboxylase (Pyc) mRNA, complete cds.

M.smegmatis gItA gene for citrate synthase.

Antarctic bacterium DS2-3R citrate synthase (cisy) gene, complete cds.

Escherichia coli K-12 MG1655 section 65 of 400 of the complete genome.

M.smegmatis gltA gene for citrate synthase.

Homo sapiens Homo sapiens Homo sapiens Bacteroides fragilis Homo sapiens Homo sapiens Mycobacterium tuberculosis Mycobacterium tuberculosis Corynebactedum glutamicurn Corynebacterium glutamicum Mycobacterium tuberculosis Corynebacteriumn glutamicumn Corynebacteriumn glutamicumn Mycobacterium tuberculosis Corynebacterium glutamicum Corynebacterium glutamicum Aspergillus terreus 36,039 35,331 35,331 39.747 39,185 35,922 57.677 37,143 100,000 100,000 37,363 99,259 99,259 41,317 100,000 100,000 03-DEC-i1999 11 -Sep-99 11-Sep-99 01 -DEC-i1999 16-Jul-99 21 -Apr-97 17-Jun-98 17-Jun-98 08-MAY-i1998 24-DEC-i1997 17-Jun-98 08-MAY-i1998 24-DEC-i1997 17-Jun-98 08-MAY-I1998 24-DEC-I997 52,248 29-OCT-1998 Mycobacterium smegmatis 58,460 Antarctic bacterium DS2- 57,154 3R Esctierichia coli 38,164 Mycobacterium smegmatis 58,929 20-Sep-91 23-Sep-97 12-Nov-98 20-Sep-91 GB-PR4:HUAC002299 171681 AC002299 Homo sapiens Chromosome 16 BAC clone CIT987-SKA-1 13A6 -complete genomic sequence, complete sequence.

Homo sapiens 33,070 23-Nov-99 2006200985 24 Feb 2006 GB)ITG2:AC007889 127840 ACD07889 rxa02399 1467 GB-BA1:CGACEA GB SAl :CORACEA GBPAT:113693 rxa02404 2340 GBBAl :CGACEB GB_8A1:CORACEB GB SAl :PFFC2 rxa02414 870 GBPR4:AC007102 GBHTG3:AC01 1214 GBHTG3;AC01 1214 rxaD2435 681 GB-BA2:AF101055

GBOM:RABPKA

GBOM:RABPLASISM

rxa02440 963 GBEST114:AA41 7723 GBEST11MA215428 GBBA1:MTCY77 rxa02453 876 GB-EST14:AA426336 GBBA1:STMAACC8 GB-PR3:AC004500 rxa02474 897 GB..BA1 :AB009078 GB-OM:8TU71200 GBEST2:F12685 rxa02480 1779 GB-BA1:MTV012 2427 1905 2135 3024 2725 5588 176258 183414 183414 7457 4441 4458 374 303 22255 375 1353 77538 2686 877 287 70287 X75504 L28760 113693 X78491 L27123 Y1 1998 AC0071 02 AC0l 1214 AC0l 1214 AF101055 J03247 M54656 AA417723 AA215428 Z95389 AA426336 M55426 AC004500 AB009078 U71 200 F1 2685 AL021287 Table 4 (continued) Drosophila melanogaster chromosome 3 clone BACR48E12 (0695) RPCI-98 Drosophila melanogaster 48.E. 12 map 87A-87B strain y; cn bw sp, -SEQUENCING IN PROGRESS-, 86 unordered pieces.

C.glutamicumn aceA gene and thiX genes (partial). Corynebacterium glutamicum Corynebacteriumn glutamicum isocitrate lyase (aceA) gene. Corynebacteriumn glutamicum Sequence 3 from patent US 5439822. Unknown.

C.glutamicumn (ATCC 13032) aceB gene. Corynebacteriumn glutamicum Corynebacteriumn glutamicum malate synthase (aceB) gene, complete cds. Corynebacterium glutamicum P.fluorescens FC2.1, FC2.2, FC2.3c, FC2.4 and FC2.5c open reading frames. Pseudomonas fluorescens Homo sapiens chromosome 4 clone C0162P16 map 4p16, complete sequence. Homo sapiens Homo sapiens clone 5_-C 3, LOW-PASS SEQUENCE SAMPLING. Homo sapiens Homo sapiens clone 5_C_3, LOW-PASS SEQUENCE SAMPLING. Homo sapiens Clostridiumn acetobutylicum atp operon, complete sequence. Clostridium acetobutylicun Rabbit phosphorylase kinase (alpha subunit) mRNA, complete cds. Oryctolagus cuniculus Oryctolagus cuniculus phosphorylase kinase alpha subunit mRl4A, complete Oryctolagus cuniculus cds.

zv0ibt2.s1 NCI O GAP_-GCB1 Homo sapiens cDNA clone IMAGE:746207 3' Homo sapiens similar to contains Alu repetitive element;contains element LI repetitive element;, mRNA sequence.

zr95a07.si NCICGAPGCB1 Homo sapiens cDNA clone IMAGE:683412 3' Homo sapiens similar to contains Alu repetitive element;, mRNA sequence.

Mycobacteriumn tuberculosis H37Rv complete genome: segment 146/1 62. Mycobacteriumn tuberculosis zv53g02.sl Soares-testisNHT H-omo sapiens cDNA clone IMAGE:757394 3% Homo sapiens mRNA sequence.

S.fradiae aminoglycoside acetyltransfe rase (aacCB) gene, complete ods. Streptomyces fradiae Homo sapiens chromosome 5, P1I clone 1076B9 (LBNL H1 complete Homo sapiens sequence.

Brevibacterium saccharolyticumn gene for L-2.3-butanediol dehydrogenase, Brevibacterium complete ods. saccharolyticum Bos taurus acetoin reductase mRNA, complete eds. Bos taurus HSC3DAO31 normalized infant brain cDNA Homo sapiens cDNA clone c- Homo sapiens 3da03, mRNA sequence Mycobacterium, tuberculosis H37Rv complete genome: segment 1321162. Mycobacteriumn tuberculosis 34,897 100,000 100,000 99,795 99,914 99,786 63.539 35,069 36,885 36,885 I39,605 36,061 36,000 38,770 39,934 38,889 38,043 37,097 33,256 96,990 51,659 41,509 35,737 2-Aug-99 9-Sep-94 10-Feb-95 26-Sep-95 13-Jan-95 8-Jun-95 1 1-Jul-97 2-Jun-99 03-OCT-i1999 03-OCT-i1999 03-MAR-i1999 27-Apr-93 22-Jun-98 1 6-OCT-i 997 13-Aug-97 18-Jun-98 16-OCT-i 997 05-MAY-i1993 30-MAR-i1998 13-Feb-99 8-Oct-97 14-Mar-95 23-Jun-99 2006200985 24 Feb 2006 rxa02485 nxa02492 840 rxa02528 1098 GB_BA1:SC6GIO GSBBAI :AP000O60 GB BA 1:STMPGM GB_BA1:MTCY20G9 GB_BA1:U00018 GBPR2:HS161N1O GB-HTG2:AC008235 36734 AL049497 347800 AP000060 921 M83661 37218 Z77162 42991 U00018 56075 AL008707 136017 AC008235 136017 AC008235 Table 4 (continued) Streptomyces coelicolor cosmid 6G1 0.

Aeropyrum pemnix genomic DNA, section 317.

Streptomyces coelicolor Aeropyrum pernix GBHTG2:AC008235 rxa02539 1641 GBBA2:RSU17129 GBBA1 :MTVO38 GB-BA2:AF068264 17425 16094 3152 rxa02551 483 OB-BAl :BACHYPTP 17057 GB -BA1 :BACHUTWAPP28954 GBBA1:BSGBGLUC 4290 rxa02556 1281 GB -HTG3:ACOO81 28 335761 GBHTG3:AC008128 335761 GB-PL2:AC005292 99053 rxa02560 990 GB-INI:CEFO7A11 35692 GBEST32:A1731605 566 GBJIN1:CEFO7A1 1 35692 U17129 AL021 933 AF068264 D29985 D31856 Z34526 AC0081 28 AC008128 AC005292 Z66511 A1731605 Z66511 Streptomyces coelicolor phosphoglycerate mutase (PGM) gene, complete cds.

Mycobacterium tuberculosis H37Rv complete genome; segment 25/162.

Mycobacterium leprae cosmid B2168.

Human DNA sequence from PAC 161 N10 on chromosome Xq25. Contains

EST.

Drosophila melanogaster chromosome 3 clone BACRiS5131 (13995) RPCI-98 15.6.19 map 94F-95A strain y; cn bw sp, SEQUENCING IN PROGRESS 125 unordered pieces.

Drosophila melanogaster chromosome 3 clone BACRi 51319 (D995) RPCI-98 15.13.19 map 94F-95A strain y; cn bw sp, -SEQUENCING IN PROGRESS-, 125 unordered pieces.

Rhodococcus erythropolis ThcA (thcA) gene, complete cds: and unknown genes.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 241162.

Pseudomonas aeruginosa quinoprotein ethanol dehydrogenase (exaA)gene.

partial cds, cytochrome c550 precursor (exa NAD+ dependent acetaldehyde dehydrogenase (exaC). and pyrroloqulnoline quinone synthesis A (pqqA) genes, complete cds; and pyrroloquinoline quinone synthesis B (pqqB) gene.

partial cds.

Bacillus subtilis wapA and orf genes for wall-associated protein and hypothetical proteins.

Bacillus subtilis genome containing the hut and wapA loci.

B.subtilis (Marburg 168) genes for bela-glucoside permease and betaglucosidase.

Homo sapiens, SEQUENCING IN PROGRESS ~,106 unordered pieces.

Homo sapiens, SEQUENCING IN PROGRESS *,106 unordered pieces.

Genomic sequence for Arabidopsis thaliana BAC F26F24, complete sequence.

Caenorhabditis elegans cosmid FO7A 1, complete sequence.

BNLGHi1O2O1 Six-day Cotton fiber Gossypiumn hirsutumn cDNA 5'similar to (AC004684) hypothetical protein (Arabidopsis thaliana], mRNA sequence.

Caenorhabditis elegans; cosmid FO7A1 1, complete sequence.

Drosophila melanogaster 35,822 Streptomyces coelicolor Mycobacterium tuberculosis Mycobacteriumn leprae Homo sapiens Drosophila melanogaster 35,511 48,014 65,672 61,436 37,893 37.051 36,822 Rhodococcus erythropolis Mycobacterium tuberculosis Pseudomonas aeruginosa Bacillus subtilis Bacillus subtilis Bacillus subtilis Homo sapiens Homo sapiens Arabidopsis thaliana Caenorhabditis elegans Gossypium hirsutum Caenorhabditis elegans 66,117 65,174 65,448 53,602 53,602 53,602 34,022 34,022 33,858 36,420 38,095 33,707 24-MAR-i1999 22-Jun-99 26-Apr-93 17-Jun-98 01-MAR-1994 23-Nov-99 2-Aug-99 2-Aug-99 16-Jul-99 17-Jun-98 1 8-MAR-1 999 7-Feb-99 7-Feb-99 3-Jul-95 22-Aug-99 22-Aug-99 16-Apr-99 2-Sep-99 11 -Jun-99 2-Sep-99 2006200985 24 Feb 2006 rxa02572 668 GB-BA1 :MTCY63 GB-BA1 :MTCY63 GB-HTG1 :HS24H01 rxa02596 1326 GB-BA1:MTV026 GBBA2:AF026540 GBBA2:MTU96128 rxa02611 1775 GBBA1:MTCY130 GBBAi:MSGY151 GBBA1:U00014 rxa02612 2316 GB-BA:MTCY13O GB3BA1:MSGYi51 GBBAi:STMGLGEN rxa02621 942 GB-BA1:CGL133719 GB INI:CEM1O6 GBEST29:A1547662 rxa02640 1650 GB-BA1:MTV025 GB-BA1:PAU49666 GB 3A1 ABO115974 rxa02654 1008 GBEST6:N65787 GBPL2:Ti 71-3 05 rxa02666 891 GB-PR3AC004643 38900 38900 46989 23740 1778 1200 32514 37036 36470 32514 37036 2557 1839 39973 377 121125 4495 1641 512 65839 88871 43411 Z96800 Z96800 AL121632 AL022076 AF026540 U96128 Z73902 AD00001 8 U00014 Z73902 AD000018 Li11647 AJ133719 Z46935 A1547662 AL0221 21 U49666 ABO1 5974 N65787 AC00591 6 U58 105 ACOD4643 Table 4 (continued) Mycobacterium tuberculosis H37Rv complete genome: segment 16/162. Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genonme; segment 16/162. Mycobacterium tuberculosis Homo sapiens chromosome 21 clone LLN Lci16H0l124 map 21 q21, Homo sapiens SEQUENCING IN PROGRESS in unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 157/1 62. Mycobacterium tuberculosis Mycobacterium tuberculosis UDP-galactopyranose mutase (91f) gene, complete Mycobacterium cdls. tuberculosis Mycobacterium tuberculosis UDP-galactopyranose mutase (glf) gene, complete Mycobacterium ccls.tuberculosis Mycobacterium tuberculosis H37Rv complete genome: segment 59/1 62. Mycobacterium tuberculosis Mycobacterium tuberculosis sequence from clone yll Mycobacterium tuberculosis Mycobacterium Ieprae cosmid B1549. Mycobacteriumn lepre Mycobacterium tuberculosis H37Rv complete genome; segment 59/162, Mycobacterium tuberculosis Mycobacterium tuberculosis sequence from clone y1 51. Mycobacterium tuberculosis Streptomyces; aureofaciens glycogen branching enzyme (g1gB) gene, complete Streptomyces ccls. aureofaciens Corynebacterium glutamicum yjcc gene, amtlR gene and citE gene, partial. Corynebacterium glutamicum Caenorhabditis elegans cosmid M106, complete sequence. Caenorhabditis elegans; UI-R-C3-sz-h-03-0-UI.sl UI-R-C3 Rattus norvegicus cDNA clone UI-R-C3-sz-h- Rattus norvegicus 03-0-Ul 3T, mRNA sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 155/1 62. Mycobacterium tuberculosis Pseudomonas aeruginosa (orfX), glycerol dfiffusion facilitator (glpF), glycerol Pseudomonas aeruginosa kinase (glpK), and Glp repressor (glpR) genes, complete cds, and (orfK) gene, partial cds.

Pseudomonas tolaasii glpK gene for glycerol kinase, complete cdls. Pseudomonas tolaasii 20827 Lambda-PRIL2 Arabidopsis thaliana cDNA clone 232B7T7, mRNA Arabidopsis thaliana sequence.

Arabidopsis thaliana chromosome 1 BAC Ti 7H3 sequence, complete Arabidopsis thaliana sequence.

Mus musculus Btk locus, alpha-D-galactosidase A (Ags), ribosomal protein Mus musculus (1-441L), and Bruton's tyrosine kinase (Btk) genes, complete cdls.

Homo sapiens chromosome 16, cosmid clone 363E3 (LANIL), complete Homo sapiens sequence.

61,677 37,170 19,820 36,957 67,627 70,417 38,532 60,575 57,486 38,018 58,510 57,193 36,858 37,608 50,667 39,187 59,273 58,339 39,637 33,735 35,431 38,851 17-Jun-98 17-Jun-98 29-Sep-99 24-Jun-99 30-OCT-i1998 25-MAR-i1998 17-Jun-98 10-DEC-i1996 29-Sep-94 17-Jun-98 1 0-DEC-i 996 25-MAY-i1995 12-Aug-99 2-Sep-99 3-Jul-99 24-Jun-99 1 8-MAY-i1997 28-Aug-99 5-Jan-98 5-Aug-99 1 3-Feb-97 01-MAY-1998 2006200985 24 Feb 2006 GB BA1 -MLCL536 GBBA1:U00013 rxa02740 1053 GB_HTG2:AC006247 GBHTG2:AC006247 GBHTG3:AC0071 50 rxa02741 1089 GB-HTG2:AC004951 GBHTG2:AC004951 GB-IN1 :AB006546 rxa02743 1161 GBBA1:MLCL536 GBBAI:U00013 36224 Z99125 35881 U00013 174368 AC006247 174368 AC006247 121474 AC007150 Table 4 (continued) Mycobacteriumn Ieprae cosmid 1536.

Mycobacteriumn Ieprae cosmid B1496.

Mycobacterium; Ieprae Mycobacterium Ieprae 61,573 04-DEC- 1998 61,573 Oi-MAR-1994 Drosophila melanogaster chromosome 2 clone BACR48110 (13505) RPCI-98 Drosophila melanogaster 48.1.10 map 49E6-49F8 strain y; cni bw sp, SEQUENCING IN PROGRESS unordered pieces.

Drosophila melanogaster chromosome 2 clone BACR48110 (0505) RPCI-98 Drosophila melanogaster 48.1.10 map 49E6-49F8 strain y; cn bw sp, SEQUENCING IN PROGRESS -,17 unordered pieces.

Drosophila melanogaster chromosome 2 clone BACR16P313 (0597) RPCI-98 Drosophila melanogaster 16.P.13 map 49E-49F strain y; cn bw sp, -SEQUENCING IN PROGRESS-, 87 unordered pieces.

37,105 37.105 38,728 33,116 33,116 36,379 48,401 48,401 2-Aug-99 2-Aug-99 20-Sep-99 12-Jun-98 12-Jun-98 23-Jun-99 04-DEC-i1998 01-MAR-i1994 129429 AC004951 Homo sapiens clone 13.1022114, SEQUENCING IN PROGRESS *1,14 Homo sapiens 129429 AC004951 931 36224 35881 AB006546 Z99125 U00013 unordered pieces.

Homo sapiens clone D.11022114, SEQUENCING IN PROGRESS 14 Homo sapiens unordered pieces.

Ephydatia fluviatilis mRNA for G protein a subunit 4, partial cds. Ephydatia fluviatilis Mycobacterium Ieprae cosmid L536. Mycobacteriumn leprae Mycobacterium leprae cosmid 81496. Mycobactedriu leprae H-omo sapiens clone NHOSO51007, SEQUENCING IN PROGRESS 3 Homo sapiens unordered pieces.

C.glutamicum betP gene. Corynebacterium glutamicum HS53136_AA03_MR CIT Approved Human Genomic Sperm Library D Homo Homo sapiens sapiens genomic clone Plate=3136 Col=5 Row-A, genomic survey sequence.

GBHTG2:AC007401 83657 AC007401 rxa02797 1026 GB-BA1:CGBETPGEN 2339 X93514 GB-GSS9:AQ148714 405 AQ148714 37,128 26-Jun-99 38,889 8-Sep-97 34,321 08-OCT-1998 GBBA1:BFU64514 GBBA1:U00020 rxa02803 680 GB-BA2:PSU85643 GBBA1:SC6G4 rxa02821 363 GB-HTG2:ACOO81 05 GB-HTG2:ACOO81 05 3837 U64514 36947 U00020 4032 U85643 41055 AL031317 91421 AC008105 91421 AC008105 Bacillus firmus dppABC operon, dipeptidle transporter protein dppA gene, partial cdls, and dipeptide transporter proteins dppB and dppC genes, complete cds.

Mycobacteriumn Ieprae cosmid 13229.

Bacillus firmus 38,072 Mycobacteriumn leprae 34,462 1-Feb-97 01-MAR-1994 Pseudomonas syringae pv. syringae putative dihydropteroate synthase gene, Pseudomonas syringae pv. 50,445 partial ods, regulatory protein MrsA (mrsA). trhose phosphate isomerase (tpiA), syningae transport protein SeoG (secG), tRNA-Leu, tRNA-Met, and 15 kDa protein genes, complete cds.

Streptomyces coelicolor cosmid 6G4. Streptomyces coelicolor 59,314 Homo sapiens chromosome 17 clone 2020_K_17 map 17, SEQUENCING Homo sapiens 37,607 IN PROGRESS 12 unordered pieces.

Homo sapiens chromosome 17 clone 2020K1,.7 map 17, SEQUENCING H-omo sapiens 37.607 IN PROGRESS 12 unordered pieces.

AVI 17143 Mus musculus C57BlI6J 10-day embryo Mus musculus cDNA clone Mus musculus 40,157 2610200J 17, mRNA sequence.

9-Apr-97 20-Aug-98 22-Jul-99 22-Jul-99 30-Jun-99 GBE5T33:AV1 17143 222 AV1I17143 -118r, Exemplification Example 1: Preparation of total genomic DNA of Corynebacterium glutamicum SATCC 13032 A culture of Corynebacterium glutamicum (ATCC 13032) was grown overnight 00 at 30 0 C with vigorous shaking in BHI medium (Difco). The cells were harvested by 0 centrifugation, the supernatant was discarded and the cells were resuspended in 5 ml Cr buffer-I of the original volume of the culture all indicated volumes have been Scalculated for 100 ml of culture volume). Composition of buffer-I: 140.34 g/1 sucrose, c 10 2.46 g/1 MgSO, x 7H 2 0, 10 ml/l KHPO, solution (100 g/l, adjusted to pH 6.7 with KOH), 50 ml/1 M12 concentrate (10 g/l 1 g/l NaCI, 2 g/l MgSO, x 7H,O, 0.2 g/1 CaCI,, 0.5 g/l yeast extract (Difco), 10 ml/l trace-elements-mix (200 mg/I FeSO, x HO, 10 mg/1 ZnSO x 7 HO 2 3 mg/1 MnCI, x 4 HO 2 30 mg/l H,BO, 20 mg/l CoCI, x 6 HO 2 1 mg/l NiCl 2 x 6 HO, 3 mg/l Na 2 MoO, x 2 H,O, 500 mg/l complexing agent (EDTA or critic acid), 100 ml/l vitamins-mix (0.2 mg/l biotin, 0.2 mg/l folic acid, mg/l p-amino benzoic acid, 20 mg/1 riboflavin, 40 mg/l ca-panthothenate, 140 mg/l nicotinic acid, 40 mg/1 pyridoxole hydrochloride, 200 mg/l myo-inositol). Lysozyme was added to the suspension to a final concentration of 2.5 mg/ml. After an approximately 4 h incubation at 37'C, the cell wall was degraded and the resulting protoplasts are harvested by centrifugation. The pellet was washed once with 5 ml buffer-I and once with 5 ml TE-buffer (10 mM Tris-HCl, 1 mM EDTA, pH The pellet was resuspended in 4 ml TE-buffer and 0.5 ml SDS solution and 0.5 ml NaCl solution (5 M) are added. After adding of proteinase K to a final concentration of 200 jtg/ml, the suspension is incubated for ca.18 h at 37 0 C. The DNA was purified by extraction with phenol, phenol-chloroform-isoamylalcohol and chloroformisoamylalcohol using standard procedures. Then, the DNA was precipitated by adding 1/50 volume of 3 M sodium acetate and 2 volumes of ethanol, followed by a 30 min incubation at -20 0 C and a 30 min centrifugation at 12,000 rpm in a high speed centrifuge using a SS34 rotor (Sorvall). The DNA was dissolved in 1 ml TE-buffer containing pg/ml RNaseA and dialysed at 4°C against 1000 ml TE-buffer for at least 3 hours.

During this time, the buffer was exchanged 3 times. To aliquots of 0.4 ml of the dialysed DNA solution, 0.4 ml of 2 M LiCI and 0.8 ml of ethanol are added. After a 119 min incubation at -20 0 C, the DNA was collected by centrifugation (13,000 rpm, Biofuge Fresco, Heraeus, Hanau, Germany). The DNA pellet was dissolved in STE-buffer. DNA prepared by this proced,ure could be used for all purposes, Sincluding southern blotting or construction of genomic libraries.

c 5 Example 2: Construction of genomic libraries in Escherichia coli of Corynebacterium glutamicum ATCC13032 o Using DNA prepared as described in Example 1, cosmid and plasmid libraries were constructed according to known and well established methods (see c-i Sambrook, J. et al. (1989) "Molecular Cloning A Laboratory Manual", Cold 0 10 Spring Harbor Laboratory Press, or Ausubel, F.M. et al. (1994) "Current Protocols cN in Molecular Biology", John Wiley Sons).

Any plasmid or cosmid could be used. Of particular use were the plasmids pBR322 (Sutcliffe, J.G. (1979) Proc. Natl. Acad. Sci. USA, 75:3737-3741); pACYC177 (Change Cohen (1978) J. Bacteriol 134:1141-1156), plasmids of the pBS series (pBSSK+, pBSSK- and others; Stratagene, LaJolla, USA), or cosmids as SuperCosl (Stratagene, LaJolla, USA) or Lorist6 (Gibson, T.J., Rosenthal A. and Waterson, R.H. (1987) Gene 53:283-286. Gene libraries specifically for use in C. glutamicum may be constructed using plasmid pSL109 (Lee, and A.J. Sinskey (1994) J. Microbiol. Biotechnol. 4:256-263).

Example 3: DNA Sequencing and Computational Functional Analysis Genomic libraries as described in Example 2 were used for DNA sequencing according to standard methods, in particular by the chain termination method using AB1377 sequencing machines (see Fleischman, R.D. et al.

(1995) "Whole-genome Random Sequencing and Assembly of Haemophilus Influenzae Rd., Science, 269:496-512). Sequencing primers with the following nucleotide sequences were used: 5'-GGAAACAGTATGACCATG-3' (SEQ ID NO.

783) or 5'-GTAAAACGACGGCCAGT-3' (SEQ ID NO. 784).

Example 4: In vivo Mutagenesis In vivo mutagenesis of Corynebacterium glutamicum can be performed by passage of plasmid (or other vector) DNA through E. coli or other microorganisms Bacillus spp. or yeasts such as Saccharomyces cerevisiae) which are impaired in their capabilities to maintain -120- (I the integrity of their genetic information. Typical mutator strains have mutations in the genes dfor the DNA repair system mutHLS, mutD, mutT, etc.; for reference, see Rupp, W.D.

(1996) DNA repair mechanisms, in: Escherichia coli and Salmonella, p. 2277-2294, ASM:

C

Washington.) Such strains are well known to those of ordinary skill in the art. The use of such strains is illustrated, for example, in Greener, A. and Callahan, M. (1994) Strategies 7: 32-34.

00 O Example 5: DNA Transfer Between Escherichia coli and Corynebacterium NC glutamicum SSeveral Corynebacterium and Brevibacterium species contain endogenous C 10 plasmids (as pHM1519 or pBLI) which replicate autonomously (for review see, e.g., Martin, J.F. et al. (1987) Biotechnology, 5:137-146). Shuttle vectors for Escherichia coli and Corynebacterium glutamicum can be readily constructed by using standard vectors for E. coli (Sambrook, J. et al. (1989), "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press or Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley Sons) to which a origin or replication for and a suitable marker from Corynebacterium glutamicum is added. Such origins of replication are preferably taken from endogenous plasmids isolated from Corynebacterium and Brevibacterium species. Of particular use as transformation markers for these species are genes for kanamycin resistance (such as those derived from the Tn5 or Tn903 transposons) or chloramphenicol (Winnacker, E.L. (1987) "From Genes to Clones Introduction to Gene Technology, VCH, Weinheim). There are numerous examples in the literature of the construction of a wide variety of shuttle vectors which replicate in both E.

coli and C. glutamicum, and which can be used for several purposes, including gene overexpression (for reference, see Yoshihama, M. et al. (1985) J. Bacteriol. 162:591-597, Martin J.F. et al. (1987) Biotechnology, 5:137-146 and Eikmanns, B.J. et al. (1991) Gene, 102:93-98).

Using standard methods, it is possible to clone a gene of interest into one of the shuftle vectors described above and to introduce such a hybrid vectors into strains of Corynebacterium glutamicum. Transformation of C. glutamicum can be achieved by protoplast transformation (Kastsumata, R. et al. (1984) J. Bacteriol. 159306-311), electroporation (Liebl, E. et al. (1989) FEMS Microbiol. Letters, 53:399-303) and in cases where special vectors are used, also by conjugation (as described e.g. in Schafer, A et al.

-121 q (1990) J. Bacteriol. 172:1663-1666). It is also possible to transfer the shuttle vectors for C. glutamicum to E. coli by preparing plasmid DNA from C glutamicum (using standard methods well-known in the art) and transforming it into E. coli. This transformation step can be performed using standard methods, but it is advantageous to use an Mcr-deficient E. coli strain, such as NM522 (Gough Murray (1983) J. Mol. Biol. 166:1-19).

00 Genes may be overexpressed in C. glutamicum strains using plasmids which 0 comprise pCGI Patent No. 4,617,267) or fragments thereof, and optionally the CN gene for kanamycin resistance from TN903 (Grindley, N.D. and Joyce, C.M. (1980)

\D

SProc. Natl. Acad Sci. USA 77(12): 7176-7180). In addition, genes may be C 10 overexpressed in C. glutamicum strains using plasmid pSL109 (Lee, and A. J.

Sinskey (1994) J Microbiol. Biotechnol. 4: 256-263).

Aside from the use of replicative plasmids, gene overexpression can also be achieved by integration into the genome. Genomic integration in C. glutamicum or other Corynebacterium or Brevibacterium species may be accomplished by well-known methods, such as homologous recombination with genomic region(s), restriction endonuclease mediated integration (REMI) (see, DE Patent 19823834), or through the use of transposons. It is also possible to modulate the activity of a gene of interest by modifying the regulatory regions a promoter, a repressor, and/or an enhancer) by sequence modification, insertion, or deletion using site-directed methods (such as homologous recombination) or methods based on random events (such as transposon mutagenesis or REMI). Nucleic acid sequences which function as transcriptional terminators may also be inserted 3' to the coding region of one or more genes of the invention; such terminators are well-known in the art and are described, for example, in Winnacker, E.L. (1987) From Genes to Clones Introduction to Gene Technology. VCH: Weinheim.

Example 6: Assessment of the Expression of the Mutant Protein Observations of the activity of a mutated protein in a transformed host cell rely on the fact that the mutant protein is expressed in a similar fashion and in a similar quantity to that of the wild-type protein. A useful method to ascertain the level of transcription of the mutant gene (an indicator of the amount of mRNA available for translation to the gene product) is to perform a Northern blot (for reference see, for example, Ausubel et al.

-122-

C

N (1988) Current Protocols in Molecular Biology, Wiley: New York), in which a primer d designed to bind to the gene of interest is labeled with a detectable tag (usually radioactive or chemiluminescent), such that when the total RNA of a culture of the organism is C extracted, run on gel, transferred to a stable matrix and incubated with this probe, the binding and quantity of binding of the probe indicates the presence and also the quantity 00 of mRNA for this gene. This information is evidence of the degree of transcription of the 0 mutant gene. Total cellular RNA can be prepared from Corynebacterium glutamicum by C several methods, all well-known in the art, such as that described in Bormann, E.R. et al.

S(1992) Mol. Microbiol. 6: 317-326.

C 10 To assess the presence or relative quantity of protein translated from this mRNA, standard techniques, such as a Western blot, may be employed (see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: New York). In this process, total cellular proteins are extracted, separated by gel electrophoresis, transferred to a matrix such as nitrocellulose, and incubated with a probe, such as an antibody, which specifically binds to the desired protein. This probe is generally tagged with a chemiluminescent or colorimetric label which may be readily detected. The presence and quantity of label observed indicates the presence and quantity of the desired mutant protein present in the cell.

Example 7: Growth of Genetically Modified Corynebacterium glutamicum Media and Culture Conditions Genetically modified Corynebacteria are cultured in synthetic or natural growth media. A number of different growth media for Corynebacteria are both well-known and readily available (Lieb et al. (1989) Appl. Microbiol. Biotechnol., 32:205-210; von der Osten et al. (1998) Biotechnology Letters, 11:11-16; Patent DE 4,120,867; Liebl (1992) "The Genus Corynebacterium, in: The Procaryotes, Volume II, Balows, A. et al., eds.

Springer-Verlag). These media consist of one or more carbon sources, nitrogen sources, inorganic salts, vitamins and trace elements. Preferred carbon sources are sugars, such as mono-, di-, or polysaccharides. For example, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch or cellulose serve as very good carbon sources. It is also possible to supply sugar to the media via complex compounds such as molasses or other by-products from sugar refinement. It can also be -123- (N4 advantageous to supply mixtures of different carbon sources. Other possible carbon d sources are alcohols and organic acids, such as methanol, ethanol, acetic acid or lactic acid. Nitrogen sources are usually organic or inorganic nitrogen compounds, or materials which contain these compounds. Exemplary nitrogen sources include ammonia gas or ammonia salts, such as NI-LCI or (NH,) 2 SO,, NH,OH, nitrates, urea, amino acids or 00 complex nitrogen sources like corn steep liquor, soy bean flour, soy bean protein, yeast extract, meat extract and others.

Inorganic salt compounds which may be included in the media include the chloride-, phosphorous- or sulfate- salts of calcium, magnesium, sodium, cobalt, 0 10 molybdenum, potassium, manganese, zinc, copper and iron. Chelating compounds can be added to the medium to keep the metal ions in solution. Particularly useful chelating compounds include dihydroxyphenols, like catechol or protocatechuate, or organic acids, such as citric acid. It is typical for the media to also contain other growth factors, such as vitamins or growth promoters, examples of which include biotin, riboflavin, thiamin, folic acid, nicotinic acid, pantothenate and pyridoxin. Growth factors and salts frequently originate from complex media components such as yeast extract, molasses, corn steep liquor and others. The exact composition of the media compounds depends strongly on the immediate experiment and is individually decided for each specific case. Information about media optimization is available in the textbook "Applied Microbiol. Physiology, A Practical Approach (eds. P.M. Rhodes, P.F. Stanbury, IRL Press (1997) pp. 53-73, ISBN 0 19 963577 It is also possible to select growth media from commercial suppliers, like standard 1 (Merck) or BHI (grain heart infusion, DIFCO) or others.

All medium components are sterilized, either by heat (20 minutes at 1.5 bar and 121 or by sterile filtration. The components can either be sterilized together or, if necessary, separately. All media components can be present at the beginning of growth, or they can optionally be added continuously or batchwise.

Culture conditions are defined separately for each experiment. The temperature should be in a range between 15"C and 45*C. The temperature can be kept constant or can be altered during the experiment. The pH of the medium should be in the range of 5 to 8.5, preferably around 7.0, and can be maintained by the addition of buffers to the media.

An exemplary buffer for this purpose is a potassium phosphate buffer. Synthetic buffers such as MOPS, HEPES, ACES and others can alternatively or simultaneously be used. It -124- -u is also possible to maintain a constant culture pH through the addition of NaOH or SNH,OH during growth. If complex medium components such as yeast extract are utilized, the necessity for additional buffers may be reduced, due to the fact that many complex c compounds have high buffer capacities. If a fermentor is utilized for culturing the microorganisms, the pH can also be controlled using gaseous ammonia.

00 The incubation time is usually in a range from several hours to several days. This 0 time is selected in order to permit the maximal amount of product to accumulate in the C broth. The disclosed growth experiments can be carried out in a variety of vessels, such as Smicrotiter plates, glass tubes, glass flasks or glass or metal fermentors of different sizes.

C 10 For screening a large number of clones, the microorganisms should be cultured in microtiter plates, glass tubes or shake flasks, either with or without baffles. Preferably 100 ml shake flasks are used, filled with 10% (by volume) of the required growth medium. The flasks should be shaken on a rotary shaker (amplitude 25 mm) using a speed-range of 100 300 rpm. Evaporation losses can be diminished by the maintenance of a humid atmosphere; alternatively, a mathematical correction for evaporation losses should be performed.

If genetically modified clones are tested, an unmodified control clone or a control clone containing the basic plasmid without any insert should also be tested. The medium is inoculated to an OD 6 00 oo of 0.5 1.5 using cells grown on agar plates, such as CM plates (10 g/l glucose, 2,5 g/l NaC1, 2 g/l urea, 10 g/1 polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l NaCI, 2 g/1 urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l agar, pH 6.8 with 2M NaOH) that had been incubated at 30'C. Inoculation of the media is accomplished by either introduction of a saline suspension of C. glutamicum cells from CM plates or addition of a liquid preculture of this bacterium.

Example 8 In vitro Analysis of the Function of Mutant Proteins The determination of activities and kinetic parameters of enzymes is well established in the art. Experiments to determine the activity of any given altered enzyme must be tailored to the specific activity of the wild-type enzyme, which is well within the ability of one of ordinary skill in the art. Overviews about enzymes in general, as well as specific details concerning structure, kinetics, principles, methods, applications and examples for the determination of many enzyme activities may be 125found, for example, in the following references: Dixon, and Webb, (1979) DEnzymes. Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism.

Freeman: New York; Walsh, (1979) Enzymatic Reaction Mechanisms. Freeman: San Francisco; Price, Stevens, L. (1982) Fundamentals of Enzymology. Oxford Univ.

Press: Oxford; Boyer, ed. (1983) The Enzymes, 3 rd ed. Academic Press: New 00 York; Bisswanger, (1994) Enzymkinetik, 2 nd ed. VCH: Weinheim (ISBN 0 3527300325); Bergmeyer, Bergmeyer, GraB1, eds. (1983-1986) Methods of Enzymatic Analysis, 3 rd ed., vol. I-XII, Verlag Chemie: Weinheim; and Ullmann's 0Encyclopedia of Industrial Chemistry (1987) vol. A9, "Enzymes". VCH: Weinheim, p.

C 10 352-363.

The activity of proteins which bind to DNA can be measured by several wellestablished methods, such as DNA band-shift assays (also called gel retardation assays).

The effect of such proteins on the expression of other molecules can be measured using reporter gene assays (such as that described in Kolmar, H. et al. (1995) EMBO J. 14: 3895-3904 and references cited therein). Reporter gene test systems are well known and established for applications in both pro- and eukaryotic cells, using enzymes such as beta-galactosidase, green fluorescent protein, and several others.

The determination of activity of membrane-transport proteins can be performed according to techniques such as those described in Gennis, R.B. (1989) "Pores, Channels and Transporters", in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p. 85-137; 199-234; and 270-322.

Example 9: Analysis of Impact of Mutant Protein on the Production of the Desired Product The effect of the genetic modification in C. glutamicum on production of a desired compound (such as an amino acid) can be assessed by growing the modified microorganism under suitable conditions (such as those described above) and analyzing the medium and/or the cellular component for increased production of the desired product an amino acid). Such analysis techniques are well known to one of ordinary skill in the art, and include spectroscopy, thin layer chromatography, staining methods of various kinds, enzymatic and microbiological methods, and analytical chromatography such as high performance liquid chromatography (see, for example, 126- CN Ullman, Encyclopedia of Industrial Chemistry, vol. A2, p. 89-90 and p. 443-613, VCH: SWeinheim (1985); Fallon, A. et al., (1987) "Applications of HPLC in Biochemistry" in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17; Rehm et al.

N1 (1993) Biotechnology, vol. 3, Chapter III: "Product recovery and purification", page 469-714, VCH: Weinheim; Belter, P.A. et al. (1988) Bioseparations: downstream 00 processing for biotechnology, John Wiley and Sons; Kennedy, J.F. and Cabral, J.M.S.

(1992) Recovery processes for biological materials, John Wiley and Sons; Shaeiwitz, J.A. and Henry, J.D. (1988) Biochemical separations, in: Ulmann's Encyclopedia of SIndustrial Chemistry, vol. B3, Chapter 11, page 1-27, VCH: Weinheim; and Dechow, S 10 F.J. (1989) Separation and purification techniques in biotechnology, Noyes Publications.) In addition to the measurement of the final product of fermentation, it is .also possible to analyze other components of the metabolic pathways utilized for the production of the desired compound, such as intermediates and side-products, to determine the overall efficiency of production of the compound. Analysis methods include measurements of nutrient levels in the medium sugars, hydrocarbons, nitrogen sources, phosphate, and other ions), measurements of biomass composition and growth, analysis of the production of common metabolites of biosynthetic pathways, and measurement of gasses produced during fermentation. Standard methods for these measurements are outlined in Applied Microbial Physiology, A Practical Approach, P.M. Rhodes and P.F. Stanbury, eds., IRL Press, p. 103-129; 131-163; and 165-192 (ISBN: 0199635773) and references cited therein.

Example 10: Purification of the Desired Product from C. glutamicum Culture Recovery of the desired product from the C glutamicum cells or supernatant of the above-described culture can be performed by various methods well known in the art.

If the desired product is not secreted from the cells, the cells can be harvested from the culture by low-speed centrifugation, the cells can be lysed by standard techniques, such as mechanical force or sonication. The cellular debris is removed by centrifugation, and the supernatant fraction containing the soluble proteins is retained for further purification of the desired compound. If the product is secreted from the C. glutamicum -127- CN cells, then the cells are removed from the culture by low-speed centrifugation, and the Ssupernate fraction is retained for further purification.

The supernatant fraction from either purification method is subjected to C chromatography with a suitable resin, in which the desired molecule is either retained on a chromatography resin while many of the impurities in the sample are not, or where the 0 impurities are retained by the resin while the sample is not. Such chromatography steps may be repeated as necessary, using the same or different chromatography resins. One i of ordinary skill in the art would be well-versed in the selection of appropriate Schromatography resins and in their most efficacious application for a particular molecule 10 to be purified. The purified product may be concentrated by filtration or ultrafiltration, and stored at a temperature at which the stability of the product is maximized.

There are a wide array of purification methods known to the art and the preceding method of purification is not meant to be limiting. Such purification techniques are described, for example, in Bailey, J.E. Ollis, D.F. Biochemical Engineering Fundamentals, McGraw-Hill: New York (1986).

The identity and purity of the isolated compounds may be assessed by techniques standard in the art. These include high-performance liquid chromatography (HPLC), spectroscopic methods, staining methods, thin layer chromatography, NIRS, enzymatic assay, or microbiologically. Such analysis methods are reviewed in: Patek et al. (1994) Appl. Environ. Microbiol. 60: 133-140; Malakhova et al. (1996) Biotekhnologiya 11: 27- 32; and Schmidt et al. (1998) Bioprocess Engineer. 19: 67-70. Ulmann's Encyclopedia of Industrial Chemistry, (1996) vol. A27, VCH: Weinheim, p. 89-90, p. 521-540, p. 540- 547, p. 559-566, 575-581 and p. 581-587; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons; Fallon, A. et al.

(1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17.

Example 11: Analysis of the Gene Sequences of the Invention The comparison of sequences and determination of percent homology between two sequences are art-known techniques, and can be accomplished using a mathematical algorithm, such as the algorithm of Karlin and Altschul (1990) Proc. Natl. Acad. Sci.

USA 87:2264-68, modified as in Karlin and Altschul (1993) Proc. Natl. Acad. Sci. USA 128- 90:5873-77. Such an algorithm is incorporated into the NBLAST and XBLAST q programs (version 2.0) of Altschul, et al. (1990) J. Mol. Biol. 215:403-10. BLAST nucleotide searches can be performed with the NBLAST program, score 100, 1 wordlength 12 to obtain nucleotide sequences homologous to SMP nucleic acid molecules of the invention. BLAST protein searches can be performed with the 00 XBLAST program, score 50, wordlength 3 to obtain amino acid sequences a homologous to SMP protein molecules of the invention. To obtain gapped alignments C for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., S(1997) Nucleic Acids Res. 25(17):3389-3402. When utilizing BLAST and Gapped 10 BLAST programs, one of ordinary skill in the art will know how to optimize the parameters of the program XBLAST and NBLAST) for the specific sequence being analyzed.

Another example of a mathematical algorithm utilized for the comparison.of sequences is the algorithm of Meyers and Miller ((1988) Comput. Appl. Biosci. 4: 11- 17). Such an algorithm is incorporated into the ALIGN program (version 2.0) which is part of the GCG sequence alignment software package. When utilizing the ALIGN program for comparing amino acid sequences, a PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used. Additional algorithms for sequence analysis are known in the art, and include ADVANCE and ADAM. described in Torelli and Robotti (1994) Comput. Appl. Biosci. 10:3-5; and FASTA, described in Pearson and Lipman (1988) P.N.A.S. 85:2444-8.

The percent homology between two amino acid sequences can also be accomplished using the GAP program in the GCG software package (available at http://www.gcg.com), using either a Blosum 62 matrix or a PAM250 matrix, and a gap weight of 12, 10, 8, 6, or 4 and a length weight of 2, 3, or 4. The percent homology between two nucleic acid sequences can be accomplished using the GAP program in the GCG software package, using standard parameters, such as a gap weight of 50 and a length weight of 3.

A comparative analysis of the gene sequences of the invention with those present in Genbank has been performed using techniques known in the art (see, Bexevanis and Ouellette, eds. (1998) Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins. John Wiley and Sons: New York). The gene sequences of the invention -129- CN were compared to genes present in Genbank in a three-step process. In a first step, a SBLASTN analysis a local alignment analysis) was performed for each of the sequences of the invention against the nucleotide sequences present in Genbank, and the I top 500 hits were retained for further analysis. A subsequent FASTA search a combined local and global alignment analysis, in which limited regions of the sequences 0o are aligned) was performed on these 500 hits. Each gene sequence of the invention was subsequently globally aligned to each of the top three FASTA hits, using the GAP C program in the GCG software package (using standard parameters). In order to obtain correct results, the length of the sequences extracted from Genbank were adjusted to the C 10 length of the query sequences by methods well-known in the art. The results of this analysis are set forth in Table 4. The resulting data is identical to that which would have been obtained had a GAP (global) analysis alone been performed on each of the genes of the invention in comparison with each of the references in Genbank, but required significantly reduced computational time as compared to such a database-wide GAP (global) analysis. Sequences of the invention for which no alignments above the cutoff values were obtained are indicated on Table 4 by the absence of alignment information.

It will further be understood by one of ordinary skill in the art that the GAP alignment homology percentages set forth in Table 4 under the heading homology (GAP)" are listed in the European numerical format, wherein a represents a decimal point. For example, a value of "40,345" in this column represents "40.345%".

Example 12: Construction and Operation of DNA Microarrays The sequences of the invention may additionally be used in the construction and application of DNA microarrays (the design, methodology, and uses of DNA arrays are well known in the art, and are described, for example, in Schena, M. et al. (1995) Science 270: 467-470; Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359-1367; DeSaizieu, A. et al. (1998) Nature Biotechnology 16: 45-48; and DeRisi, J.L. et al.

(1997) Science 278: 680-686).

DNA microarrays are solid or flexible supports consisting of nitrocellulose, nylon, glass, silicone, or other materials. Nucleic acid molecules may be attached to the surface in an ordered manner. After appropriate labeling, other nucleic acids or nucleic acid mixtures can be hybridized to the immobilized nucleic acid molecules, and the label -130- (N may be used to monitor and measure the individual signal intensities of the hybridized Smolecules at defined regions. This methodology allows the simultaneous quantification of the relative or absolute amount of all or selected nucleic acids in the applied nucleic C1 acid sample or mixture. DNA microarrays, therefore, permit an analysis of the expression of multiple (as many as 6800 or more) nucleic acids in parallel (see, e.g., 0 Schena, M. (1996) BioEssays 18(5): 427-431).

The sequences of the invention may be used to design oligonucleotide primers which are able to amplify defined regions of one or more C. glutamicum genes by a 0nucleic acid amplification reaction such as the polymerase chain reaction. The choice CI 10 and design of the 5' or 3' oligonucleotide primers or of appropriate linkers allows the covalent attachment of the resulting PCR products to the surface of a support medium described above (and also described, for example, Schena, M. et al. (1995) Science 270: 467-470).

Nucleic acid microarrays may also be constructed by in situ oligonucleotide synthesis as described by Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359- 1367. By photolithographic methods, precisely defined regions of the matrix are exposed to light. Protective groups which are photolabile are thereby activated and undergo nucleotide addition, whereas regions that are masked from light do not undergo any modification. Subsequent cycles of protection and light activation permit the synthesis of different oligonucleotides at defined positions. Small, defined regions of the genes of the invention may be synthesized on microarrays by solid phase oligonucleotide synthesis.

The nucleic acid molecules of the invention present in a sample or mixture of nucleotides may be hybridized to the microarrays. These nucleic acid molecules can be labeled according to standard methods. In brief, nucleic acid molecules mRNA molecules or DNA molecules) are labeled by the incorporation of isotopically or fluorescently labeled nucleotides, during reverse transcription or DNA synthesis.

Hybridization of labeled nucleic acids to microarrays is described in Schena, M. et al. (1995) supra; Wodicka, L. et al. (1997), supra; and DeSaizieu A. et al. (1998), supra). The detection and quantification of the hybridized molecule are tailored to the specific incorporated label. Radioactive labels can be detected, for example, as 131 Cl described in Schena, M. et al. (1995) supra) and fluorescent labels may be detected, for Sexample, by the method of Shalon et al. (1996) Genome Research 6: 639-645).

The application of the sequences of the invention to DNA microarray Cl technology, as described above, permits comparative analyses of different strains of C.

glutamicum or other Corynebacteria. For example, studies of inter-strain variations 00 based on individual transcript profiles and the identification of genes that are important for specific and/or desired strain properties such as pathogenicity, productivity and stress tolerance are facilitated by nucleic acid array methodologies. Also, comparisons Sof the profile of expression of genes of the invention during the course of a fermentation reaction are possible using nucleic acid array technology.

Example 13: Analysis of the Dynamics of Cellular Protein Populations (Proteomics) The genes, compositions, and methods of the invention may be applied to study the interactions and dynamics of populations of proteins, termed 'proteomics'. Protein populations of interest include, but are not limited to, the total protein population of C.

glutamicum in comparison with the protein populations of other organisms), those proteins which are active under specific environmental or metabolic conditions during fermentation, at high or low temperature, or at high or low pH), or those proteins which are active during specific phases of growth and development.

Protein populations can be analyzed by various well-known techniques, such as gel electrophoresis. Cellular proteins may be obtained, for example, by lysis or extraction, and may be separated from one another using a variety of electrophoretic techniques. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins largely on the basis of their molecular weight. Isoelectric focusing polyacrylamide gel electrophoresis (IEF-PAGE) separates proteins by their isoelectric point (which reflects not only the amino acid sequence but also posttranslational modifications of the protein). Another, more preferred method of protein analysis is the consecutive combination of both IEF-PAGE and SDS-PAGE, known as 2-D-gel electrophoresis (described, for example, in Hermann et al. (1998) Electrophoresis 19: 3217-3221; Fountoulakis et a. (1998) Electrophoresis 19: 1193-1202; Langen et al.

(1997) Electrophoresis 18: 1184-1192; Antelmann et al. (1997) Electrophoresis 18: 132- N 1451-1463). Other separation techniques may also be utilized for protein separation, such as capillary gel electrophoresis; such techniques are well known in the art.

Proteins separated by these methodologies can be visualized by standard Stechniques, such as by staining or labeling. Suitable stains are known in the art, and include Coomassie Brilliant Blue, silver stain, or fluorescent dyes such as Sypro Ruby 0 (Molecular Probes). The inclusion of radioactively labeled amino acids or other protein 00 0precursors 35 S-methionine, 35 S-cysteine, 1 4 C-labelled amino acids, 15 N-amino acids, 15NO3 or "NH 4 or 3 C-labelled amino acids) in the medium of C. glutamicum IN permits the labeling of proteins from these cells prior to their separation. Similarly, fluorescent labels may be employed. These labeled proteins can be extracted, isolated and separated according to the previously described techniques.

Proteins visualized by these techniques can be further analyzed by measuring the amount of dye or label used. The amount of a given protein can be determined quantitatively using, for example, optical methods and can be compared to the amount of other proteins in the same gel or in other gels. Comparisons of proteins on gels can be made, for example, by optical comparison, by spectroscopy, by image scanning and analysis of gels, or through the use of photographic films and screens. Such techniques are well-known in the art.

To determine the identity of any given protein, direct sequencing or other standard techniques may be employed. For example, N- and/or C-terminal amino acid sequencing (such as Edman degradation) may be used, as may mass spectrometry (in particular MALDI or ESI techniques (see, Langen et al. (1997) Electrophoresis 18: 1184-1192)). The protein sequences provided herein can be used for the identification of C. glutamicum proteins by these techniques.

The information obtained by these methods can be used to compare patterns of protein presence, activity, or modification between different samples from various biological conditions different organisms, time points of fermentation, media conditions, or different biotopes, among others). Data obtained from such experiments alone, or in combination with other techniques, can be used for various applications, such as to compare the behavior of various organisms in a given metabolic) situation, to increase the productivity of strains which produce fine chemicals or to increase the efficiency of the production of fine chemicals.

133

SEQUIVALENTS

-qj SThose of ordinary skill in the art will recognize, or will be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

00 Comprises/comprising and grammatical variations thereof when used in this specification are to be taken to specify the presence of stated features,

ID

0 10 integers, steps or components or groups thereof, but do not preclude the C presence or addition of one or more other features, integers, steps, components or groups thereof.

Claims (47)

1. An isolated Corynebacterium glutamicum nucleic acid molecule comprising a nucleic acid sequence selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 781 provided that the nucleic acid molecule does not consist of any t of the F-designated genes set forth in Table 1, or a complement thereof. 00

2. An isolated nucleic acid molecule which encodes a polypeptide comprising an amino Iacid sequence selected from the group consisting of each of the even numbered SEQ (iID NOs; 4 to 782, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

3. An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 782, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

4. An isolated nucleic acid molecule comprising a nucleotide sequence which is at least identical to the entire nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 781, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof. An isolated nucleic acid molecule comprising a fragment of at least 15 contiguous nucleotides of the nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 781, provided that the nucleic acid molecule does not consist of any of the F designated genes set forth in Table 1, or a complement thereof.

6. An isolated nucleic acid molecule comprising the nucleic acid molecule of any one of claims 1-5 and a nucleotide sequence encoding a heterologous polypeptide. c 7. A vector comprising the nucleic acid molecule of any one of claims 1-6.

8. The vector of claim 7, which is an expression vector.

9. A host cell transfected with the expression vector of claim 8. 00 O 10. The host cell of claim 9, wherein said cell is a microorganism. \O S11. The host cell of claim 10,. wherein said cell belongs to the genus Corynebacterium or Brevibacterium.

12. The host cell of claim 9, wherein the expression of said nucleic acid molecule results in the modulation in production of a fine chemical from said cell.

13. The host cell of claim 12, wherein said fine chemical is selected from the group consisting of: organic acids, proteinogenic and nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides, and enzymes.

14. A method of producing a polypeptide, the method comprising culturing the host cell of claim 9 in an appropriate culture medium to, thereby, produce the polypeptide. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 782, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1.

16. An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of c each of the even numbered SEQ ID NOs: 4 to 782, provided that the amino acid Ssequence is not encoded by any of the F-designated genes set forth in Table 1. 1 17. An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 50% identical to the entire nucleic acid sequence 00 of any of the odd numbered SEQ ID NOs: 3 to 781, provided that the nucleic acid O molecule does not consist of any of the F-designated nucleic acid molecules set forth 6 in Table 1.

18. An isolated polypeptide comprising an amino acid sequence which is at least identical to the entire amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 782, provided that the amino acid sequence is not encoded by any of the F- designated genes set forth in Table 1.

19. An isolated polypeptide comprising a fragment of a polypeptide comprising the amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 782, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino sequence. An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 781, provided that the nucleic acid molecule does not consist of any of the F-designated nucleic acid molecules set forth in Table 1.

21. The isolated polypeptide of any one of claims 15-20, further comprising heterologous amino acid sequences.

22. A method for producing a fine chemical, the method comprising culturing the cell of claim 9 such that the fine chemical is produced. (I 23. The method of claim 22, wherein said method further comprises the step of recovering the fine chemical from said culture.

24. The method of claim 22, wherein said cell belongs to the genus Corynebacterium or Brevibacterium. 00 8 25. The method of claim 22, wherein said cell is selected from the group consisting of: "Corynebacterium glutamicum, Corynebacterium herculis, Corynebacterium, lilium, Corynebacterium acetoacidophilum, Corynebacterium acetoglutamicum, Corynebacterium acetophilum, Corynebacterium ammoniagenes, Corynebacterium fujiokense, Corynebacterium nitrilophilus, Brevibacterium ammoniagenes, Brevibacterium butanicum, Brevibacterium divaricatum, Brevibacteriumflavum, Brevibacterium healii, Brevibacterium ketoglutamicum, Brevibacterium ketosoreductum Brevibacterium lactofermentum, Brevibacterium linens, Brevibacterium parciffinolyticum and those strains set forth in Table 3.

26. The method of claim 22, wherein expression of the nucleic acid molecule from said vector results in modulation of production of said fine chemical.

27. The method of claim 22, wherein said fine chemical is selected from the group consisting of: organic acids, proteinogenic and nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides and enzymes.

28. The method of claim 22, wherein said fine chemical is an amino acid.

29. The method of claim 28, wherein said amino acid is drawn from the group consisting of: lysine, glutamate, glutamine, alanine, aspartate, glycine, serine, threonine, N methionine, cysteine, valine, leucine, isoleucine, arginine, proline, histidine, tyrosine, Sphenylalanine, and tryptophan. A method for producing a fine chemical, comprising culturing a cell whose genomic DNA has been altered by the introduction of a nucleic acid molecule of any one of 00 claims 1-6. C

31. A method for diagnosing the presence or activity of Corynebacterium diphtheriae in a Ssubject, the method comprising detecting the presence of at least one of the nucleic acid molecules of any one of claims 1-5 or the polypeptide molecules of any one of claims 15-20, thereby diagnosing the presence or activity of Corynebacterium diphtheriae in the subject.

32. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 781, wherein the nucleic acid molecule is disrupted.

33. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 781, wherein the nucleic acid molecule comprises one or more nucleic acid modifications as compared to the sequence set forth in any of the odd-numbered SEQ ID NOs: 3 to 781.

34. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 781, wherein the regulatory region of the nucleic acid molecule is modified relative to the wild-type regulatory region of the molecule. An isolated Corynebacterium glutamicum nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO: 179, or a complement thereof. N 36. An isolated nucleic acid molecule which encodes a polypeptide comprising the amino Sacid sequence set forth in SEQ ID NO: 180, or a complement thereof. C 37. An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID No: 180, or 00 a complement thereof. N

38. An isolated nucleic acid molecule comprising a nucleotide sequence which is at least identical to the entire nucleotide sequence set forth in SEQ ID No: 179, or a complement thereof.

39. An isolated nucleic acid molecule comprising a fragment of at least 15 contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO: 179, or a complement thereof. An isolated nucleic acid molecule comprising the nucleic acid molecule of any one of claims 35-39 and a nucleotide sequence encoding a heterologous polypeptide.

41. A vector comprising the nucleic acid molecule of any one of claims 35-40.

42. The vector of claim 41, which is an expression vector.

43. A host cell transfected with the expression vector of claim 42.

44. The host cell of claim 43, wherein said cell is a microorganism. The host cell of claim 44, wherein said cell belongs to the genus Corynebacterium or Brevibacterium. N 46. The host cell of claim 43, wherein the expression of said nucleic acid molecule results Sin the modulation in production of a fine chemical from said cell. S47. The host cell of claim 44, wherein said fine chemical is selected from the group consisting of: organic acids, proteinogenic and nonproteinogenic amino acids, purine 00 and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty 0 acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides, and enzymes. O

48. A method of producing a polypeptide comprising culturing the host cell of claim 43 in an appropriate culture medium to, thereby, produce the polypeptide.

49. An isolated polypeptide comprising the amino acid sequence set forth in SEQ ID NO:180. An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO: 180.

51. An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 50% identical to the entire nucleotide sequence set forth in SEQ ID NO:179.

52. An isolated polypeptide comprising an amino acid sequence which is at least identical to the entire amino acid sequence set forth in SEQ ID NO:180.

53. An isolated polypeptide comprising a fragment of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:180, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino sequence set forth in SEQ ID NO:180. C1 54. An isolated polypeptide which is encoded by a nucleic acid molecule comprising a Snucleotide sequence as set forth in SEQ ID NO: 179. C

55. The isolated polypeptide of any one of claims 49-54, further comprising heterologous amino acid sequences. 00 O 56. A method for producing a fine chemical, the method comprising culturing the cell of C claim 43 such that the fine chemical is produced.

57. The method of claim 56, wherein said method further comprises the step of recovering the fine chemical from said culture.

58. The method of claim 56, wherein said cell belongs to the genus Corynebacterium or Brevibacterium.

59. The method of claim 56, wherein said cell is selected from the group consisting of: Corynebacterium glutamicum, Corynebacterium herculis, Corynebacterium, lilium, Corynebacterium acetoacidophilum, Corynebacterium acetoglutamicum, Corynebacterium acetophilum, Corynebacterium ammoniagenes, Corynebacterium fujiokense, Corynebacterium nitrilophilus, Brevibacterium ammoniagenes, Brevibacterium butanicum, Brevibacterium divaricatum Brevibacterium flavum, Brevibacterium healit, Brevibacterium ketoglutamicum, Brevibacterium ketosoreductum, Brevibacterium lactofermentum, Brevibacterium linens, Brevibacterium paraffinolyticum, and those strains set forth in Table 3. The method of claim 56, wherein expression of the nucleic acid molecule from said vector results in modulation of production of said fine chemical.

61. The method of claim 56, wherein said fine chemical is selected from the group consisting of: organic acids, proteinogenic and nonproteinogenic amino acids, purine c and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty Sacids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides and enzymes.

62. The method of claim 56, wherein said fine chemical is an amino acid. 00 O 63. The method of claim 62, wherein said amino acid is drawn from the group consisting C of: lysine, glutamate, glutamine, alanine, aspartate, glycine, serine, threonine, Smethionine, cysteine, valine, leucine, isoleucine, arginine, proline, histidine, tyrosine, phenylalanine, and tryptophan.

64. A method for producing a fine chemical, the method comprising culturing a cell whose genomic DNA has been altered by the introduction of a nucleic acid molecule of any one of claims 35-40. A method for diagnosing the presence or activity of Corynebacterium diphtheriae, the method comprising detecting the presence of at least one of the nucleic acid molecules of any one of claims 35-39 or the polypeptide molecules of any one of claims 49-54, thereby diagnosing the presence or activity of Corynebacterium diphtheriae.

66. A host cell comprising the nucleic acid molecule set forth in SEQ ID NO: 179, wherein the nucleic acid molecule is disrupted.

67. A host cell comprising the nucleic acid molecule set forth in SEQ ID NO: 179, wherein the nucleic acid molecule comprises one or more nucleic acid modifications as compared to the sequence set forth in SEQ ID NO: 179.

68. A host cell comprising the nucleic acid molecule set forth in SEQ ID NO: 179, wherein the regulatory region of the nucleic acid molecule is modified relative to the wild-type regulatory region of the molecule.

69. A polypeptide produced by the method of claim 14. A fine chemical produced by the method of any one of claims 22 to 31 or 56 to 64. DATED this 24th day of February 2006 BASF AKTIENGESELLSCHAFT WATERMARK PATENT TRADE MARK ATTORNEYS 290 BURWOOD ROAD HAWTHORN VICTORIA 3122 AUSTRALIA P20679AU01 Gene Sequence 24-2-06. txt SEQUENCE LI STI NG <110> BASF Akt i engesel I schaf t <120> CORYNEBACTERI UM GLLTAM CUM GENES ENCODI NG PROTEI NS I NVOLVED I N CARBCN IVETABCLI SM AND ENERGY PRODUCTI CN <130> BGI -126CPPC <140> PCT/I BOO/00943 <141> 2000-06-23 <150> 60/141,031 <151> 1999-06-25 <150> 60/143,208 <151> 1999-07-09 <150> 60/151,572 <151> 1999-08-31 <150> DE 19931412.8 <151> 1999-07-08 <150> DE 19931413.6 <151> 1999-07-08 <150> DE 19931419.5 <151> 1999-07-08 <150> DE 19931420.9 <151> 1999-07-08 <150> DE 19931424.1 <151> 1999-07-08 <150> DE 19931428.4 <151> 1999-07-08 <150> DE 19931431.4 <151> 1999-07-08 <150> DE 19931433.0 <151> 1999-07-08 <150> DE 19931434.9 <151> 1999-07-08 <150> DE 19931510.8 <151> 1999-07-08 <150> DE 19931562.0 <151> 1999-07-08 <150> DE 19931634. 1 <151> 1999-07-08 <150> DE 19932180.9 <151> 1999-07-09 <150> DE 19932227.9 <151> 1999-07-09 <150> DE 19932230.9 <151> 1999-07-09 <150> DE 19932924.9 Page 1 Gene Sequence 24-2-06.txt <151> 1999-07-14 <150> DE 19932973.7 <151> 1999-07-14 <150> DE 19933005.0 <151> 1999-07-14 <150> DE 19940765.7 <151> 1999-08-27 <150> DE 19942076.9 <151> 1999-09-03 <150> DE 19942079.3 <151> 1999-09-03 <150> DE 19942086.6 <151> 1999-09-03 <150> DE 19942087.4 <151> 1999-09-03 <150> <151> <150> <151> <150> <151> <150> <151> DE 19942088.2

1999-09-03 DE 19942095.5 1999-09-03 DE 19942123.4 1999-09-03 DE 19942125.0 1999-09-03 <160> 784 <210> 1 <211> 828 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(805) <223> RXS02735 <400> 1 gaggagcttc gccacatgga tccagatttg ggctaccagc agcgtcaagc tggaaaccgt ctaaggagaa atacaacact acgcactatc cggcttgtcc atg gtt gat gta Net Val Asp Val 1 cgc gca cgc gat Arg Ala Arg Asp act Thr gaa gat ttg gtt G u Asp Leu Val gca Al a 15 cag gct gcc tcc G n Ala Ala Ser aaa ttc Lys Phe att gag gtt I e G u Val gaa gca gca act G u Al a Ala Thr gcc Al a aat aat ggc acc Asn Asn y Thr gca cag gta Al a G n Val aag ctc agc Lys Leu Ser gtg ctc acc ggt ggt ggc gcc ggc atc aag ttg ctg gaa Val Leu Thr Gy Gy y Ala Gy lie Lys Leu Leu u 45 gtt gat gcg gct gac ctt gcc tgg gat Val Asp Ala Ala Asp Leu Ala Trp Asp cgc att Arg Ile Page 2 cat gtg ttc ttc ggc His Val Phe Phe y Gene Sequence 24- 2- 06. txt 60 gat gag cgc aat gtc cct gtc agt gat tct gag tcc aat gag ggc cag 355 Asp G u Arg Asn Val Pro Val Ser Asp Ser u Ser Asn G u G y n 75 80 gct cgt gag gca ctg ttg tcc aag gtt tct atc cct gaa gcc aac att 403 Ala Arg u Ala Leu Leu Ser Lys Val Ser lie Pro u Ala Asn Ile 95 100 cac gga tat ggt ctc ggc gac gta gat ctt gca gag gca gcc cgc gct 451 His G y Tyr G y Leu G y Asp Val Asp Leu Ala G u Ala Ala Arg Ala 105 110 115 tac gaa gct gtg ttg gat gaa ttc gca cca aac ggc ttt gat ctt cac 499 Tyr G u Ala Val Leu Asp G u Phe Ala Pro Asn G y Phe Asp Leu His 120 125 130 ctg ctc ggc atg ggt ggc gaa ggc cat atc aac tcc ctg ttc cct cac 547 Leu Leu G y Iet G y G y G u G y His I I e Asn Ser Leu Phe Pro His 135 140 145 acc gat gca gtc aag gaa tcc tcc gca aag gtc atc gcg gtg ttt gat 595 Thr Asp Ala Val Lys u Ser Ser Ala Lys Val I e Ala Val Phe Asp 150 155 160 165 tcc cct aag cct cct tca gag cgt gca act cta acc ctt cct gcg gtt 643 Ser Pro Lys Pro Pro Ser G u Arg Ala Thr Leu Thr Leu Pro Ala Val 170 175 180 cac tcc gca aag cgc gtg tgg ttg ctg gtt tct ggt gcg gag aag gct 691 His Ser Ala Lys Arg Val Trp Leu Leu Val Ser G y Ala G u Lys Ala 185 190 195 gag gca gct gcg gcg atc gtc aac ggt gag cct gct gtt gag tgg cct 739 G u Ala Ala Ala Ala I le Val Asn Gy G u Pro Ala Val G u Trp Pro 200 205 210 gct gct gga gct acc gga tct gag gaa acg gta ttg ttc ttg gct gat 787 Ala Ala y Ala Thr G y Ser G u G u Thr Val Leu Phe Leu Ala Asp 215 220 225 gat gct gca gga aat ctc taagcagcgc cagctctaac aag 828 Asp Al a Al a G y Asn Leu 230 235 <210> 2 <211> 235 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 2 IVet Val Asp Val Val Arg Ala Arg Asp Thr u Asp Leu Val Ala G n 1 5 10 Ala Ala Ser Lys Phe I e G u Val Val G u Ala Ala Thr Ala Asn Asn 25 Sy Thr Ala n Val Val Leu Thr Gy Gy Gy Ala Gy Ile Lys Leu 40 Leu G u Lys Leu Ser Val Asp Ala Ala Asp Leu Ala Trp Asp Arg lie 55 His Val Phe Phe y Asp G u Arg Asn Val Pro Val Ser Asp Ser u 70 75 Page 3 Gene Sequence 24 Ser Asn G u Gy Gn Ala Arg G u Ala Leu Leu 90 Pro G u Ala Asn lie His G y Tyr G y Leu G y 100 105 G u Ala Ala Arg Ala Tyr G u Ala Val Leu Asp 115 120 G y Phe Asp Leu His Leu Leu G y Vet G y G y 130 135 Ser Leu Phe Pro His Thr Asp Ala Val Lys G u 145 150 155 Ile Ala Val Phe Asp Ser Pro Lys Pro Pro Ser 165 170 Thr Leu Pro Ala Val His Ser Ala Lys Arg Val 180 185 G y Al a G u Lys Al a G u Al a Al a Al a Al a Ile 195 200 Ala Val u Trp Pro Al a Ala y Ala Thr y 210 215 Leu Phe Leu Ala Asp Asp Ala Ala G y Asn Leu 225 230 235 <210> 3 <211> 468 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(445) <223> RXA01626 <400> 3 gcaatagcga atgcgtaatt aaccacactt caaacctagc acattaccgt ctgaaaaatt tcatccgtag gctaaagagc -2-06. txt Ser Lys Val Asp Val Asp 110 G u Phe Al a 125 G u G y Hi s 140 Ser Ser Al a G u Arg Ala Trp Leu Leu 190 Val Asn y 205 Ser G u G u 220 Ser Leu Pr o SIe Lys Thr 175 Val G u Thr at c SIle at c SIle cag G n ggg Sy ct g Leu cgc Ar g gag G u tgc Cys gt c Val aac Asn gaa G u cag G n at g Net act Thr cca Pr o gaa G u ctt Leu cgc Ar g gca Al a gaa G u cct Pr o cca Pr o at t SIle gtt Val gtt Val gga cac G y Hi s 15 tct gag Ser G u gaa ct a G u Leu att ggc I e G y ccc cat Pro Hi s 80 Page 4 ccctcaggtg gaggattccg atg tcg aaa acg atc IVet Ser Lys Thr Ile 1 cca acc gcc atc cat Pro Thr Ala lle His gct caa ggc ggc gtg Al a n G y G y Val gca gga act gcc gaa Al a y Thr Al a G u gga tct aat ttc cag G y Ser Asn Phe G n cca gat acc tat gcg Pro Asp Thr Tyr Ala Gene Seq gat ttc ccc gat atc aag gcg gtt gtt Asp Phe Pro Asp Ile Lys Ala Val Val ggc ctg tgg ctg gaa gcg gga gcg aag G y Leu Trp Leu G u Al a y Al a Lys 105 110 taaccacttg cagtataccc tag <210> 4 <211> 115 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 4 IVet Ser Lys Thr Ile I e Val Arg Thr 1 5 Pro Thr Ala lie His Ile Ala G u IVet Al a n G y G y Val G n Vet Cys Lys 40 Ala y Thr Ala Gu y Asp Val Val 55 G y Ser Asn Phe G n Leu Asn Asn G u 70 Pro Asp Thr Tyr Ala Asp Phe Pro Asp Ala G u Thr Phe G u G y Leu Trp Leu 100 105 G y Leu Asn 115 luence 24-2-06. t xt atc tcc gcg gag act ttt gaa Ile Ser Ala u Thr Phe G u 95 100 ttc cct ggg tta aat Phe Pro G y Leu Asn 115 SIle G u G n Sy Asn Lys Al a G u Leu Ar g Al a a u Al a Sy SIle Pr o SIle Sy Val Val Al a Sy Ser G u SIe Pr o SIe Phe <210> <211> 780 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(757) <223> RXA02245 <400> gccacaccgc cacggcaccg atgcgatgtt tgtggcagtg tgagctaagt ggggtagaca agagggctat gatttagggc cca cta atc gcc cca tcc att ctt gct gct gat Pro Leu Ile Ala Pro Ser lie Leu Ala Ala Asp 15 gag cag gtg ttg gct gtt cct gat gct gac tgg G u G n Val Leu Ala Val Pro Asp Ala Asp Tr p 30 atg gac gga cac ttc gtt cca aac ttg agc ttt Page ctgcgaaaga agtagacctg atg gca caa cgt act 115 IVet Ala G n Arg Thr 1 ttc tcc cgc tta ggg 163 Phe Ser Arg Leu y att cac gtc gac atc 211 Ile His Val Asp Ile ggc gcg gat atc aca 259 IVbt Asp y Hi s Phe Val Gene Sequence 24- 2- 06. txt Pro Asn Leu Ser Phe Gy Ala Asp lie Thr 45 gct gcg gtc aac cgc gtt acg gac aaa Ala Ala Val Asn Arg Val Thr Asp Lys atc gaa aac cca gag aag tgg gtg gac Ile G u Asn Pro G u Lys Trp Val Asp 75 gac tgc att gtt ttc cac gtt gaa gcc Asp Cys Ile Val Phe His Val G u Ala gct aag tac atc cgt tcc aag ggt gtg Ala Lys Tyr Ile Arg Ser Lys y Val 105 110 cct gga act ccc atc gag gat tac ttg Pro y Thr Pro lie G u Asp Tyr Leu 120 125 gaa gtc atc gtc atg agc gtc gag cct G u Val lie Val IVet Ser Val G u Pro 135 140 atg cct gaa caa ct gaa aag gtt cgt IVet Pro G u G n Leu G u Lys Val Arg 150 155 gag cgc ggt ctg aac acc gtc atc gag Su Arg Gy Leu Asn Thr Val Ile u 170 aag acc atc aag cag gct gcc gac gct Lys Thr Ile Lys G n Al a Ala Asp Ala 185 190 ggt tcc gct gtg tac ggc gct gag gat Gy Ser Ala Val Tyr Gy Ala u Asp 200 205 ttg cga gca ctc gcg cag taaatggatg Leu Arg Ala Leu Ala G n 215 <210> 6 <211> 219 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 6 IVet Ala n Arg Thr Pro Leu Ile Ala 1 5 Phe Ser Arg Leu y G u G n Val Leu 25 li e His Val Asp I I e IVet Asp G y Hi s 40 Sy Ala Asp Ile Thr Ala Ala Val Asn 55 Asp Val His Leu Iet I e G u Asn Pro 70 gaa G u aac Asn acc Thr 95 cgt Ar g gat Asp gga G y acc Thr at c SIle 175 ggc G y ccc ct a Leu t ac Tyr 80 gaa G u gca Al a gac Asp ttc Phe ct g Leu 160 gac Asp gt g Val aac gt c Val gac Asp cac Hi s ttc Phe gag G u 130 ggc G y aag Lys ggc G y gcc Al a gcg Al a 210 cac Hi s gct Al a gtt Val t cc Ser 115 cac Hi s caa G n gt c Val at t SIle ttc Phe 195 at c l Ie ct g Leu ggc G y gag G u 100 ct g Leu ttc Phe agc Ser at c SIle agc Ser 180 gtt Val cag G n at g bet gcg Al a ttg Leu cgc Ar g gat Asp ttc Phe gat Asp 165 gcc Al a gca Al a gag G u Pro Asn Lys :tgcgcacgc gtt Pro Ser lie Leu Ala Ala Asp 10 Ala Val Pro Asp Ala Asp Trp Phe Val Pro Asn Leu Ser Phe Arg Val Thr Asp Lys G u Leu G u Lys Trp Val Asp Asn Tyr 75 Page 6 Gene Sequence 24 Ile Asp Ala Gy Ala Asp Cys lie Val Phe His 90 G y His Val G u Leu Ala Lys Tyr lie Arg Ser 100 105 Sy Phe Ser Leu Arg Pro y Thr Pro Ile G u 115 120 Leu G u His Phe Asp G u Val lie Val Iet Ser 130 135 Gy Gy n Ser Phe IVet Pro u n Leu u 145 150 155 Arg Lys Val lie Asp G u Arg y Leu Asn Thr 165 170 Gy Gy lie Ser Ala Lys Thr Ile Lys n Al a 180 185 Asp Ala Phe Val Ala G y Ser Ala Val Tyr y 195 200 Lys Ala lie Gn G u Leu Arg Ala Leu Ala G n 210 215 <210> 7 <211> 442 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(442) <223> RXA01015 <400> 7 ccaagattga gtcgagctat tttgcgattt gtgaaccccc tatccgccgt tgtgaaaatg cctgcagtaa actgacttcc -2-06. txt Val u Ala Lys G y Val 110 Asp Tyr Leu 125 Val u Pro 140 Lys Val Ar g Val Ile G u Al a Asp Al a 190 Al a u Asp 205 Thr Ar g Asp Gy Thr SIe 175 Gy Pr o gga Gy ctt Leu gat Asp aca Thr aac Asn ctt Leu gac Asp gcc Al a gaa G u aac Asn gag G u tgg Tr p gct Al a ggc Gy gac Asp cca Pr o at c SIle gtt Val ggt Gy cac Hi s tac Tyr ggc Gy gcc Al a 75 gaa G u ttc Phe gaa G u cca Pr o t ca Ser gcc Al a act Thr gaa G u gt g Val gcc Al a 45 ct c Leu aac Asn gca Al a act Thr at c SIle ttc Phe ggc Gy aag Lys cgc Ar g aaa aat Lys Asn 15 gac tgc Asp Cys tgc at c Cys I I e at c gt c Ile Val gtc aag Val Lys 80 ctc gcc Leu Al a Page 7 aaatagggga aaagtccggg atg cgc gta tac ctt IVet Arg Val Tyr Leu 1 gca atc gca gaa cac Ala I I e Ala G u His gga gcc cac acc tat G y Al a Hi s Thr Tyr gaa gca gct agc cgc G u Ala Ala Ser Arg ctg ggt gga tcc gga Leu G y G y Ser G y ggt gca cgt tgt gca Sy Ala Arg Cys Ala cgc gag cac aac aat Arg u His Asn Asn Gene Sequence 24- 2- 06. txt gcg aac ctc atc ggc atc ggc ggc cgc atg cac tca gag Ala Asn Leu Ile Gy lie Gy Gy Arg IVet His Ser u 105 110 <210> 8 <211> 114 <212> PRT <213> Corynebacter i um gl ut am cum <400> 8 IVet Arg Val Tyr Leu y Ala Asp His 1 5 Ala lle Ala u His Leu Lys Ala His G y Ala His Thr Tyr Asp Ala G u Asp 40 G u Ala Ala Ser Arg Thr Val Asn Asp 55 Leu G y G y Ser G y Asn G y G u G n 70 Sy Ala Arg Cys Ala Leu Ala Trp Ser Arg u His Asn Asn Ala Asn Leu Ile 100 105 Ser G u Al a 10 Sy Asp Pr o SIle Val 90 Sy Sy Hi s Tyr Sy Al a G u lie Phe G u Pr o Ser Al a Thr Sy G u Val Al a Leu Asn Al a Sy Lys Asp Cys SIe Val Leu bet <210> 9 <211> 2142 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(2119) <223> RXN01312 <400> 9 gcagttgcgt acatcgttcc tgcactggtc ctgatcggca atcgctgttg gttggattgc gtaaaggtta ggaagaattt gaa G u gt c Val acc Thr cca acc Thr gct Al a gat Asp aac cca Pr o acg Thr tgg Tr p cgc gag G u gt c Val gaa aau aag ttc Phe ctt Leu t ac Tyr ttc at c SIle gac Asp caa G n 45 cgt cac Hi s gct Al a aaa Lys gt c cca gt c Pro Val 15 gca gag Al a G u gac cac Asp Hi s ct c gt c Page 8 acatcaccat tccgttcgcc atg agc act cac tct IVet Ser Thr His Ser 1 tca gtc ctc cca gag Ser Val Leu Pro G u cca gca ggc gtt ccc Pro Ala G y Val Pro atg aac ctg gtc tcc IVet Asn Leu Val Ser gtt ggc acc ggc ctg Gene Sequence 24- 2- 06. txt Pro Leu Asn Arg Arg Lys Phe Arg Val Leu Val Val y Thr y Leu 60 t cc Ser aag Lys gca Al a ggc Gy ggt Gy at c SIle 150 ggc G y t ac Tyr t cc Ser cat Hi s cgc Ar g 230 gca Al a gt g Val at g let cag G n acc Thr 310 ggt Sy ttc Phe ggt Gy t ac Tyr 120 gag G u cac Hi s ttg Leu acc Thr ct a Leu 200 gaa G u gaa G u acc Thr cac Hi s gca Al a 280 cac Hi s ct g Leu gca Al a t ac Tyr gtt Val cac Hi s gac Asp aac Asn acc Thr 170 gga G y cgc Ar g gtt Val ct g Leu cat Hi s 250 t cc Ser gaa G u acc Thr t cc Ser gca Al a 75 cac Hi s aac Asn gt c Val tgc Cys gcc Al a 155 cgt Ar g caa G n cag G n gac Asp at c SIle 235 gcc Al a acc Thr gcc Al a ggc Gy gag 31u 315 gca Al a gac Asp t cc Ser aag Lys tgg Tr p 140 at c l Ie t cc Ser acc Thr at c SIle gt c Val 220 at g Met gtt Val ct g Leu ggc Gy ct g Leu 300 tcg Ser gcc Al a gca Al a gcc Al a gac Asp 125 cgt Ar g ggt Gy ttc Phe gga G y cac Hi s 205 at t SIle cgc Ar g at c SIle gcc Al a gca Al a 285 cct Pr o ct g Leu ct c Leu cct Pr o cgc Ar g 110 acc Thr ct c Leu gca Al a ggt G y cag G n 190 ct c Leu gt c Val aac Asn ct g Leu aag Lys 270 t ac Tyr gt g Val cgt Ar g ggc G y cgc Ar g 95 ggc Gy gt c Val gcc Al a cca Pr o ggt G y 175 cag G n ggc G y acc Thr ct g Leu gca Al a 255 aac Asn ttc Phe aac Asn aac Asn gaa G u 80 cgt Ar g aag Lys aag Lys gt c Val ttc Phe 160 gt g Val ct g Leu t cc Ser gaa G u at c SIle 240 acc Thr t cc Ser gcg Al a t cc Ser gac Asp 320 ct c Leu gcg Al a aag Lys ggc Gy gag G u 145 gcc Al a cag G n cag G n gt a Val cgt Ar g 225 acc Thr ggt G y aac Asn t cc Ser acc Thr 305 ggc G y gga G y cac Hi s gt a Val ggc G y 130 t cc Ser cgc Ar g gt c Val ttc Phe gaa G u 210 aac Asn ggc G y ggc G y gcc Al a cca Pr o 290 tgg Tr p cgc Ar g gac Asp at t SIle 100 aac Asn t ac Tyr cgc Ar g t ac Tyr cgt Ar g 180 acc Thr ttc Phe gaa G u ct c Leu ggc G y 260 gcc Al a ttc Phe t cc Ser tgg Tr p gt c Val gct Al a gac Asp cgt Ar g gt c Val ggt G y 165 acc Thr gca Al a acc Thr aag Lys acc Thr 245 aac Asn at c SIe at c SIe aag Lys t cc Ser 325 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 cct aag gaa ccg aac gat aac cgc gat cca aac acc atc cct gag gat Page 9 Pro Lys u Pro Asn Asp Asn 330 Gene Sequence 24-2-06.txt Arg Asp Pro Asn Thr lie Pro u Asp gag G u gt c Val ggt Gy gac Asp 390 aac Asn agc Ser tgg Tr p ggc G y t cc Ser 470 at c SIle gat Asp gac Asp gga Gy tgt Cys 550 at c SIle agc Ser gac Asp cgt Ar g 360 ggt Gy acc Thr ttc Phe at g Net gac Asp 440 gca Al a ct c Leu aac Asn cca Pr o ct c Leu 520 gag G u gtt Val gcc Al a gat Asp ttc Phe gtt Val gga Gy cgc Ar g at g Net 410 at t SI e aac Asn tgg Tr p gct Al a ct c Leu 490 gca Al a ggc Gy t ac Tyr cga Ar g cgc Ar g 570 at g bet ct g Leu gct Al a cct Pr o ct c Leu 395 tac Ty r gca Al a gaa G u acc Thr t cc Ser 475 ggc G y cag G n aac Asn cac Hi s aac Asn 555 gat Asp aac Asn gag G u t cc Ser ct g Leu 380 gga Gy gaa G u ccg Pr o at g et t ac Tyr 460 gt c Val cca Pr o gca Al a cgc Ar g cgc Ar g 540 gt a Val gac Asp cag G n cgc Ar g cgt Ar g 365 aac Asn cag G n gag G u acc Thr acg Thr 445 cac Hi s gat Asp ttg Leu gcg Al a cca Pr o 525 cag G n gaa G u ttc Phe gtt Val cgc Ar g 350 gcg Al a aac Asn gac Asp gca Al a tgc Cys 430 t ca Ser ggt G y ggc G y ctt Leu at t l Ie 510 gag G u ctt Leu gac Asp tgg Tr p ct c Leu 590 335 t ac Tyr at c l Ie gct Al a acc Thr at t l Ie 415 cac Hi s ct c Leu gca Al a tgg Tr p ggc G y 495 gcg Al a tgg Tr p ggc G y ct c Leu aag Lys 575 gaa G u cca Pr o t cc Ser gca Al a at c SIle 400 ggc G y ttc Phe cca Pr o aac Asn ttc Phe 480 t cc Ser cgt Ar g gt c Val gat Asp cag G n 560 aac Asn t ac Tyr gca Al a cag G n t ac Tyr 385 cgc Ar g gag G u acc Thr ggt G y cgt Ar g 465 acc Thr gag G u gca Al a ggt G y at c SIle 545 gat Asp at g Net gca Al a ttc Phe cag G n 370 ct g Leu gag G u gac Asp at g Net ct g Leu 450 ct g Leu ct g Leu cgt Ar g cag G n gac Asp 530 ct g Leu ggc G y cgc Ar g gca Al a 340 ggt G y 355 at c SIle gac Asp cgt Ar g cca Pr o ggt G y 435 ttc Phe ggc G y cca Pr o ct g Leu gct Al a 515 aac Asn t ac Tyr at c SIle at c SIle cgc Ar g 595 aac Asn aat Asn ttc Phe t ac Tyr t ac Tyr 420 ggc G y tgc Cys gca Al a ttc Phe t ca Ser 500 cgc Ar g gtt Val ttc Phe aac Asn acc Thr 580 gt a Val ct c Leu gct Al a cgc Ar g t cc Ser 405 t cc Ser ct c Leu gca Al a aac Asn acc Thr 485 gag G u at t SIe cac Hi s t cc Ser aag Lys 565 ggc G y gcc Al a 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 gac tac atc gac ctc ggc gaa ctc atg tgt gtc gac gcc ctc gac cgc Page Asp Tyr I Ie Asp Leu G31y (31u Leu 600 605 gac gag t cc t gt ggc gct cac t tc c As p G3 u Ser Cys G3 y Al a Hi s Phe P 615 620 ggc gaa gca caa cgt gac gac caa a G3 y G3 u Al a G31n Ar g Asp Asp (31 n 630 635 gaa cca ggc gag aat gga acc ttc g a31u Pr o 31y a31u Asn a31y Thr Phe\ 650 t tc gaa t ct gt c cca ct g cag aca a Phe a31u Ser Val Pr o Leu a31n Thr P 665 6 t acact tgag at c <210> <211> 673 <21 2> PRT <21 3> Cor ynebact er i urn gl ut ari cumn Sequence 24-2- 06. txt /bt Cys Val Asp Ala Leu Asp Arg 610 gc gac gac cac ct C t cc gaa gat ~rg Asp Asp Hi s Leu Ser a31u Asp 625 .ac t gg t gc t tc gt c t cc gca t gg osn Tr p Cys Phe Val Ser Al a Tr p 640 645 itc t gc cac gca gaa cca ct g t tc (al Cys Hi s Ala a31u Pr o Leu Phe 655 660 .gg aac tac aag t aat gaaact ~rg Asn Tyr Lys 1 987 2035 2083 2129 2142 <400> l'bt Ser Thr Hi s Ser a31u Thr Thr Ser Pr o ValI Le u Al a a31y a31u 145 Al a a31n a31n Le u a31y Le u Thr Tyr Ser As p 115 As p ValI a31u Ser Ser 1 95 a31u Pr o Ser Le u ValI Al a As p Ar g ValI a31y 1 65 Thr Al a Ser Lys5 Le u a31y Al a a31n Al a Ar g 135 As p Al a Tyr Al a Al a As p 40 As n a31y Ph e a31y Tyr 120 a31u Hi s Le u Thr Le u 200 Ar g a31y vb t Ar g Al a Thr a31y 1 05 Ar g Ser vb t Al a Ar g 1 85 a31n a31u ValI a31u Lys5 Al a Hi s As n ValI Cys Al a 1 Ar g a31n a31n I Ie As p a31n Ar g Al a Al a Al a As p 125 Ar g a31y Ph e a31y Hi s 205 Hi s Al a Lys5 ValI Le u Pr o Ar g 110 Thr Le u Al a a31y a31n 1 Le u Pr a Al a As p Le u a31y Ar g a31y ValI Al a Pr a a31y 1 a31n a31y Val a31u I Ie Phe Thr Hi s Asn a31u l'bt Val Asp Val I Ie Val Thr a31u Page 11 Gene Sequence 24-2-06.txt Ar g 225 Thr Gy Asn Ser Thr 305 Gy Thr Al a G n Tyr 385 Ar g G u Thr Gy Ar g 465 Thr G u Al a Gy SIle 545 Gy G u Tyr Ser 275 Ser G n SIle Pr o Gy 355 I le Asp Ar g Pr o Gy 435 Phe Gy Pr o Leu Al a 515 Asn Tyr Lys Thr 245 Asn SIle SIle Lys Ser 325 Asp Leu Al a Ar g Ser 405 Ser Leu Al a Asn Thr 485 G u l Ie Hi s Ser Lys 565 Ar g 230 Al a Val bet G n Thr 310 Pr o G u Val Gy Asp 390 Asn Ser Tr p Gy Ser 470 I le Asp Asp Gy Cys 550 215 Cys Hi s Tyr Ar g Phe 295 I le Lys Ar g Pr o Leu 375 Al a Leu Pr o Thr G u 455 Leu Pr o Al a Ar g Pr o 535 Gy G u G y Leu Gy bet 265 Tyr Pr o et Pr o Tyr 345 Asp Val G u Thr Ar g 425 Phe Ser Ser Tyr G u 505 let Tyr Ser Hi s 250 Ser G u Thr Ser Asn 330 Phe Val Gy Ar g et 410 I le Asn Tr p Al a Leu 490 Al a Gy Tyr Ar g Ar g 570 220 Ile bet 235 Al a Val Thr Leu Al a G y G y Leu 300 Su Ser 315 Asp Asn Leu G u Al a Ser Pro Leu 380 Leu G y 395 Tyr G u Ala Pr o G u let Thr Tyr 460 Ser Val 475 G y Pro G n Al a Asn Arg His Ar g 540 Asn Val 555 SIle Al a Al a 285 Pr o Leu Ar g Ar g Ar g 365 Asn G n G u Thr Thr 445 Hi s Asp Leu Al a Pr o 525 G n G u Leu Lys 270 Tyr Val Ar g Asp Ar g 350 Al a Asn Asp Al a Cys 430 Ser Gy Gy Leu l Ie 510 G u Leu Asp Arg Asn Leu Al a Thr 255 Asn Ser Phe Al a Asn Ser Asn Asp 320 Pro Asn 335 Tyr Pro lie Ser Al a Al a Thr Ile 400 Se G y 415 Hi s Phe Leu Pro Al a Asn Trp Phe 480 G y Ser 495 Al a Ar g Trp Val Sy Asp Leu G n 560 Lys Asn 575 Asp y Ile Asn Ile Arg Ala Leu Asp Asp Phe Trp Page 12 Met Al a Asp Hi s 625 Phe Al a Lys SIle Ar g 595 Leu Ser Ser Pr o Gy Al a Ar g Asp Tr p 645 Phe Thr Tyr G u 615 G u Pr o G u Gene Sequence 24-2-06.txt Asp G u Iet Asn G n Val Leu 585 590 I e Asp Leu G y G u Leu IVet 600 605 Ser Cys G y Ala His Phe Arg 620 Al a n Arg Asp Asp G n Asn 635 Sy u Asn y Thr Phe Val 650 Ser Val Pro Leu G n Thr Arg 665 670 G u Cys Asp Tr p Cys 655 Asn <210> 11 <211> 1100 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (1)..(1077) <223> FRXA01312 <400> 11 gag tcg ct g G u Ser Leu 1 gat aac cgc Asp Asn Arg ctg gag cgc Leu G u Arg gct t cc cgt Al a Ser Ar g cct ct g aac Pro Leu Asn ct c gga cag Leu G y G n tac gaa gag Tyr G u G u gca ccg acc Al a Pro Thr 115 aac gac ggc cgc atc tgg Asn Asp y Arg lie Trp 5 10 cca aac acc atc cct gag Pro Asn Thr lie Pro u tac cca gca ttc ggt aac Tyr Pro Ala Phe y Asn 40 atc tcc cag cag atc aat Ile Ser n G n Ile Asn gct gca tac ctg gac ttc Ala Ala Tyr Leu Asp Phe 70 acc atc cgc gag cgt tac Thr I e Arg G u Arg Tyr 90 att ggc gag gac cca tac lie G y u Asp Pro Tyr 105 cac ttc acc atg ggt ggc His Phe Thr et Gy y 120 tcc cct aag gaa ccg aac Ser Pro Lys u Pro Asn gat Asp ct c Leu gct Al a cgc Ar g t cc Ser t cc Ser ct c Leu cgc Ar g cca Pr o ct c Leu gcc Al a ct c Leu cca Pr o act Thr 125 t ac Tyr gac Asp gtt Val gag G u acc Thr cgt Ar g ttc Phe gaa atg acg tca ctc cca G u Iet Thr Ser Leu Pro 130 ggt Gy 135 ctg ttc tgc gca Leu Phe Cys Al a gaa gca tcc tgg G u Ala Ser Trp Page 13 ene Sequence 24- 2- 06. txt ac c Th r 145 t cc Ser ggc G31y c ag O31n aac As n c ac Hi s 225 aac As n g at As p aac As n ggc G31y gct A a 305 g ac As p gga G31y ct g Le u c ac Hi s g at As p t tg Le u gcg A a 1 95 cca Pr o c ag O31n g aa O31u t tc Ph e gt t ValI 275 ct c Le u t tc Ph e c aa O31n t tc Ph e ac a Th r 355 ggt gca aac cgt G31y Ala Asn Ar g 150 ggc t gg t tc acc G31y Tr p Phe Thr 1 65 ct t ggc t cc gag Leu 03 y Ser 03 u 1 80 at t gcg cgt gca Ilie Ala Ar g Ala gag t gg gt c ggt O31u Tr p Val G31y 215 ct t ggc gat at c Leu 03 y Asp Il e 230 gac ct C cag gat Asp Leu 03 n Asp 245 t gg aag aac at g Tr p Lys Asn I'b t 260 ct C gaa t ac gca Leu 03 u Tyr A a at g t gt gt c gac [Vb t Cys Val Asp 295 cgc gac gac cac Ar g Asp Asp Hi s 310 aac t gg t gc tt c Asn Trp Cys Phe 325 gt c t gc cac gca Val Cys Hi s Ala 340 agg aac tac aag Arg Asn Tyr Lys ct g ggc gca Leu 03 y A a aac t cc ct g ct c t cc ct g Le u cgt Ar g c ag O31n 200 g ac As p ct g Le u ggc G31y cgc Ar g gca A a 280 gcc A a ct c Le u gt c ValI g aa O31u cca Pr o ct g Le u 1 85 gct A a aac As n t ac Tyr at c I Ie at c I Ie 265 cgc Ar g ct c Le u t cc Ser t cc Ser cca Pr o 345 t tc Ph e 1 70 t ca Ser cgc Ar g gt t ValI t tc Ph e aac As n 250 ac c Thr gt a ValI g ac As p g aa O31u gca A a 330 ct g Le u Asn Ser 1 55 acc at c Thr I Ie gag gat 31u As p at t gac I I e As p cac gga Hi s 03 y 220 t cc t gt Ser Cys 235 aag at c Lys I I e ggc agc O3 y Ser gcc gac A a As p cgc gac Ar g As p 300 gat ggc As p (1y 315 t gg gaa Trp O31u t tc tt c Phe Phe Leu Leu Ser cct Pr a gca A a cgc Ar g 205 cct Pr a G31y cgt Ar g ac c Thr t ac Tyr 285 gag O31u g aa O31u cca Pr a g aa O31u aac As n cca Pr a 1 ct c Le u gag O31u gt t ValI gcc A a g at As p 270 at c I Ie t cc Ser gca A a ggc G31y t ct Ser 350 t ac Tyr 1 g aa O31u at g vb t t ac Tyr t cc Ser ct c Le u 255 gag O31u g ac As p t gt Cys c aa O31n gag O31u 335 gt c ValI gct A a 1 ct c Le u gca A a ggc G31y t ac Tyr cga Ar g 240 cgc Ar g at g vb t ct c Le u ggc G31y cgt Ar g 320 aat As n cca Pr a 480 528 576 624 672 720 768 816 864 912 960 1 008 1 056 1100 t aat gaaact t acact tgag at c <210O> 12 <21 1> 359 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 12 Ou Ser Leu Arg Asn Asp Gy Arg i~e Trp Ser Pro Lys Ou Pro Asn 1 5 10 Asp Asn Arg Asp Pro Asn Thr Ilie Pro Ou Asp Ou Arg AspTyr Phe Page 14 Gene Sequence 24-2-06.txt G y Asn Leu Val Arg Asp Val Leu Al a Pr o Leu Tyr Al a G u Thr 145 Ser Gy G n Asn Hi s 225 Asn Asp Asn Gy Al a 305 Asp Sy Leu Ar g Ar g Asn G n G u Thr 115 Thr Hi s Asp Leu Al a 195 Pr o G n G u Phe Val 275 Leu Phe G n Phe Thr 355 Tyr SIle Al a Thr I le Hi s Leu Al a Tr p 165 Sy Al a Tr p Sy Leu 245 Lys G u Cys Asp Tr p 325 Cys Asn Pr o Ser Al a 70 I le Sy Phe Pr o Asn 150 Phe Ser Ar g Val Asp 230 G n Asn Ty r Val Asp 310 Cys Hi s Ty r Al a G n Tyr Ar g G u Thr Sy 135 Ar g Thr G u Al a Sy 215 I le Asp let Al a Asp 295 Hi s Phe Al a Lys Phe 40 G n Leu G u Asp bet 120 Leu Leu Leu Ar g G n 200 Asp Leu Sy Ar g Al a 280 Al a Leu Val G u SIle Asp Ar g Pr o 105 Sy Phe Sy Pr o Leu 185 Al a Asn Tyr SIle SIle 265 Ar g Leu Ser Ser Pr o 345 Asn Phe Tyr 90 Tyr Sy Cys Al a Phe 170 Ser Ar g Val Phe Asn 250 Thr Val Asp G u Al a 330 Leu Al a Ar g Ser Ser Leu Al a Asn 155 Thr Su I e Hi s Ser 235 Lys Sy Al a Ar g Asp 315 Tr p Phe Pr o Leu Al a Leu Pr o Thr 125 G u Leu Pr o Al a Ar g 205 Pr o Sy Ar g Thr Tyr 285 G u G u Pr o G u Sy Thr Phe let 110 Asp Al a Leu Asn Pr o 190 Leu G u Val Al a Asp 270 I le Ser Al a Sy Ser 350 Val G u Thr Ar g Phe Ser Ser Tyr 175 G u let Tyr Ser Leu 255 G u Asp Cys G n G u 335 Val <210> 13 <211> 1593 Page Gene Sequence 24-2-06.txt <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(1570) <223> RXN00231 <400> 13 caggactacc tcgacgccgg cgccaacttt gtccgagtcg gtgccgatgt ccagcaactc aacgctgctg gatacgaaaa gtgaaggaaa ataacgcatc act att aat Thr Ile Asn ttc Phe tgg Tr p acg Thr ct g Leu at g Met gt a Val ggc G y ttc Phe gga Gy gtt Val 150 gct Al a ct c Leu acc Thr ct a Leu gaa G u gaa G u gct Al a gcg Al a gaa G u cct Pr o cgc Ar g 120 aca Thr ccg Pr o cgc Ar g cca Pr o ctt Leu 200 gt c Val t cc Ser at c SIle gat Asp gag G u gca Al a gct Al a ttc Phe gaa G u ct c Leu gt c Val 170 cga Ar g gcc Al a aaa Lys gac Asp gca Al a gct Al a cgt Ar g 75 gaa G u gaa G u tct Ser ggc Gy ct g Leu 155 gca Al a ctt Leu ggt Gy agt Ser ggc Gy acg Thr gca Al a tct Ser gag G u gct Al a gag G u aac Asn 140 at c SIle cct Pr o acc Thr ctt Leu ccc Pr o ggt Gy ct c Leu 45 tgc Cys aat Asn ttc Phe cgc Ar g gaa G u 125 ttg Leu acc Thr gcg Al a ccg Pr o cca Pr o 205 acg Thr act Thr gcg Al a gct Al a at t SIle gcc Al a ggc G y 110 gca Al a cgg Ar g cca Pr o at c l Ie ct g Leu 190 gca Al a ct a Leu gat Asp gct Al a cag G n cgc Ar g 80 ct c Leu gt c Val cgt Ar g ct g Leu aac Asn 160 gca Al a t cc Ser gt c Val at t SIle gaa Gu t cc Ser gag G u ggt G y acc Thr t ac Tyr t at Tyr 130 gcc Al a cca Pr o tgt Cys t at Tyr aat Asn 210 gat Asp cca Pr o gat Asp gct Al a gag G u gaa Gu 100 aac Asn cgt Ar g aag Lys gca Al a at g bet 180 gct Al a gt c Val gtt Val t cc Ser gcg Al a gca Al a agg Ar g ct c Leu at g let gaa Gu t ac Tyr cca Pr o at g let 165 gtg Val cag G n t cc Ser ggt gct tcc gcc tct gcg att tcc aac ccg att atg gaa gac gat cgc Page 16 G y Al aSer Al aSer Al a Il e ene Sequence 24- 2- 06. txt Ser Asn Pro Ilie I'bt Gu Asp Asp Arg 215 ct t cgt Leu Arg 230 ct C aaa Leu Lys ggc aac 31y As n atc gaa I Ie G31u acc gca Thr Al a 295 ggc cgt G31y Ar g 310 ct C gac Leu Asp gac agc Asp Ser gt c ct c Val Leu cca acg Pr o Thr 375 gag at c G31u I Ie 390 gaa gcc a3 u Al a gt g tt c Val Phe gag tt c G3 u Phe gca cct Al a Pr o 455 ct C gaa Leu G3 u 470 aaa Ly s aag Ly s gca Al a ggt a31y 280 gcc Al a cgc Ar g g aa a31u gt t ValI ac c Thr 360 gt g ValI tt c Ph e ct g Le u ac c Thr ggc a31y 440 tt t Ph e gga a31y t cc Ser gcc Al a 250 t tc Ph e at g vb t cgt Ar g gct Al a gt c ValI 330 t cg Ser ggc a31y ac g Thr ccc Pr o ct a Le u 410 g ac As p gt g ValI ggc a31y gag a31u t t c Ph e 235 g at As p at t I Ie ggt a31y t t c Ph e gcc Al a 315 acc Thr ct t Leu aag Ly s gga a31y gt c ValI 395 gcc Al a acc Thr ggc a31y gt a ValI gag a31u 475 220 ac c Thr aaa Ly s gt c ValI gcc Al a t ta Le u 300 cgc Ar g gt g ValI gt c ValI gcc Al a gt t ValI 380 gca Al a aac As n t ca Ser gt c ValI aaa Ly s 460 t ac Tyr ggc a31y gt t ValI t tc Ph e aaa Ly s 285 gt c ValI ct t Le u ggc a31y g ac As p ggc a31y 365 t ca Ser ccg Pr o t cc Ser cgt Ar g aat As n 445 c aa a31n ac c Thr t cc Ser ct g Le u gag a31u 270 at g vb t c ac Hi s g aa a31u ccc Pr o gcc Al a 350 ac a Thr ac a Thr at c I Ie ac c Thr at t I Ie 430 t cc Ser t cc Ser t cc Ser ac c Thr cgc Ar g 255 g ac As p cgc Ar g g aa a31u gag a31u ct g Le u 335 gcc Al a ggt a31y g at As p gt c ValI g aa a31u 415 tt c Ph e ggt a31y gga a31y gt g ValI cca Pr a 240 ac c Thr gcc Al a aac As n t cc Ser c aa a31n 320 gt t ValI gt c ValI gca Al a gcg Al a ac c Thr 400 t ac Tyr cgc Ar g gt c ValI at g vb t c ag a31n 480 225 gt t ValI t cc Ser g ac As p at c I Ie gt c ValI 305 gt c ValI gag a31u gcc Al a ggc a31y gct Al a 385 tt c Ph e gga a31y gt c ValI at c a31y 465 t ac Tyr ggc a31y at g vb t ct a Le u ggc a31y 290 gcc Al a ct a Le u g aa a31u g aa a31u t ac Tyr 370 at c I Ie c aa a31n ct g Le u t cc Ser t ct Ser 450 cgc Ar g at c I Ie c ag a31n g aa a31u g at As p 275 g aa a31u g at As p ggc a31y aaa Ly s ggt a31y 355 tt c Ph e ct g Le u ac c Thr gcc Al a g at As p 435 aac As n g aa a31u ggt a31y c ag a31n ct t Le u 260 ct c Le u gct Al a g aa a31u aac As n gca Al a 340 gcc Al a t ac Tyr aac As n gag a31u t cc Ser 420 ggt a31y gct Al a ggt a31y at c I Ie ct g Le u 245 ggt a31y gcg Al a t gc cys Ph e ggc a31y 325 cga Ar g ac c Thr g aa a31u g aa a31u g aa a31u 405 t at Tyr ct c Le u gct Al a ggt a31y Ar g 485 835 883 931 979 1 027 1 075 1123 1171 1219 1267 1315 1363 1411 1459 1 507 1 555 1 593 gat cct t ac gcc ggc t agcat ct gc ccct t tacaa at c Page 17 Gene Sequence 24-2-06.txt Asp Pro Tyr Ala Gy 490 <210> 14 <211> 490 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 14 let Thr Ile Asn Val Phe G u Leu Leu 1 Leu Val Thr Al a Ar g et Thr Leu Thr 145 Phe Gy G n Leu let 225 Val Ser Asp SIle Gy Asn G u Tr p Phe Leu Gy 115 Gy Leu Leu Val Phe 195 Val Asp G n G u Asp 275 G u 5 Ser Al a Al a Ar g Leu let G u Tyr Pr o et 165 Val G n Ser Ar g Leu 245 Gy Al a Cys Val Gy Al a Pr o Al a Lys Leu Thr 135 Gy Thr Lys let Al a 215 Ar g Lys Asn G u Al a 295 G u G u 40 Al a Al a G u Pr o Ar g 120 Thr Pr o Ar g Pr o Leu 200 Ser Lys Lys Al a Gy 280 Al a Al a Thr Leu Ar g Ar g Leu 105 Tr p Pr o Cys Lys Al a 185 Asp Al a Val Al a Pr o 265 Al a Asn Val 10 Ser SIle Asp G u Al a 90 Al a Phe G u Leu Val 170 Ar g Al a Ser Ser Al a 250 Phe let Ar g Lys Asp Al a Al a Ar g 75 G u G u Ser Gy Leu 155 Al a Leu Gy Al a Phe 235 Asp SIle Gy Phe Ser Gy Thr Al a Ser G u Al a G u Asn 140 I le Pr o Thr Leu l Ie 220 Thr Lys Val Al a Leu 300 Pr o Gy Leu Cys Asn Phe Ar g G u 125 Leu Thr Al a Pr o Pr o 205 Ser Gy Val Phe Lys 285 Val Thr Thr Al a Al a SIle Al a Gy 110 Al a Ar g Pr o l Ie Leu 190 Al a Asn Ser Leu G u 270 Het Hi s Gy Phe Ser Val Leu Thr G u Val et Tr p Al a 175 Thr Gy Pr o Thr Ar g 255 Asp Ar g G u Leu Asp Al a G n Ar g Leu Val Ar g Leu Asn 160 Al a Ser Val SIe Pr o 240 Thr Al a Asn Ser Val Ala Asp u Phe y Arg Arg Phe Ala Ala Arg Leu u u n Page 18 Gene Sequence 24-2-06.txt 305 Val G u Al a Gy Al a 385 Phe Gy Val SIle Gy 465 Tyr 315 Thr Val Gy Pro Leu G y G u Lys G u G y 355 Tyr Phe 370 II e Leu G n Thr Leu Al a Ser Asp 435 Ser Asn 450 Arg G u I e G y Asn y 325 Al a Ar g 340 Al a Thr Tyr G u Asn u G u G u 405 Ser Tyr 420 G y Leu Al a Al a G y G y li e Ar g 485 Leu Asp G u G y Asp Val Pr o G u 390 G u Val G u Al a Leu 470 Asp Ser Leu Thr 375 I le Al a Phe Phe Pr o 455 G u Pr o Al a 345 Gy Leu Gy Ar g G n 425 Leu Gy SIle Al a Leu Lys Gy Val 395 Al a Thr Gy Val G u 475 Val Al a Val 380 Al a Asn Ser Val Lys 460 Tyr Asp Gy 365 Ser Pr o Ser Ar g Asn 445 G n Thr Al a 350 Thr Thr SIle Thr SIle 430 Ser Ser Ser 320 Leu Val 335 Al a Val G y Al a Asp Al a Val Thr 400 G u Tyr 415 Phe Arg G y Val G y let Val G n 480 <210> <211> 870 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(847) <223> RXA01311 <400> tcgtctccgc atgggaacca ggcgagaatg gaaccttcgt tcttcgaatc tgtcccactg cagacaagga actacaagta gag G u gt c Val gac Asp ttc Phe tgg Tr p gtt Val gt a Val tct Ser caa G n gac Asp aac Asn tgc Cys gca Al a gcc Al a aag Lys cgc Ar g ggc G y gt c Val ttc Phe gaa G u cca Pr o gcg Al a at c SIle 45 ggc Gy act Thr cag G n gaa G u att SIle gcg gaa Al a G u 15 atg t cc Met Ser gaa ggc G u Gy tgt ggt Cys G y Page 19 ctgccacgca gaaccactgt atg aaa ctt aca ctt IVet Lys Leu Thr Leu 1 ggc aag ttc gaa acc G y Lys Phe G u Thr atc ctg gag ctg ctt Ile Leu G u Leu Leu aaa gaa cca ttc gcg Lys G u Pro Phe Ala acc tgt ggt ctc ctc Thr Cys G y Leu Leu Gene Sequence 24-2-06.txt gt g Val cag G n ct g Leu t cc Ser gca Al a gca Al a 150 gtt Val ctt Leu cgt Ar g t cc Ser acc Thr 230 ggc Gy ggt cgc G y Arg ctg gt c Leu Val t cc gcc Ser Al a 105 ctg gac Leu Asp 120 acc gca Thr Al a ctc gca Leu Al a gcc tgc Al a Cys cac ct c Hi s Leu 185 cgt aag Arg Lys 200 tac ggc Tyr G y gtg gca Val Ala gac gac Asp Asp cct Pr o agc Ser gca Al a cgt Ar g cct Pr o ctt Leu cct Pr o 170 t cc Ser at g Net gag Gu gct Al a ggc gcc gac cag G y Al a Asp G n aag gaa ggc gac Lys G u G y Asp 95 cca gtg atc aag Pro Val Ile Lys 110 atg gaa cag ggt eVt G u Gn y 125 gct gat acc ct c Ala Asp Thr Leu 140 cac gca gcc tgc Hi s Al a Al a Cys ggc gca gca cac G y Al a Al a Hi s 175 ct c cca ct g ggt Leu Pro Leu y 190 gat gaa atg gaa Asp G u Vet u 205 gca gat gtc tgc Ala Asp Val Cys 220 acc aag gaa cgt Thr Lys G u Arg aac Asn 80 acc Thr gac Asp ggc G y cac Hi s at c SIle 160 ct g Leu aag Lys acc Thr ccc Pr o gcg Al a 240 aag cct gcc tgt gcg Lys Pro Ala Cys Ala ctc aag atc gaa cca Leu Lys lie G u Pro 100 atg gtc gtc gac cgc et Val Val Asp Arg 115 tac gtg acc atc aac Tyr Val Thr Ile Asn 130 gtc aac cac gaa acc Val Asn His G u Thr 145 ggc tgt ggc gca tgt G y Cys G y Al a Cys 165 ttc acc ggc gca aag Phe Thr G y Al a Lys 180 gaa gag cgc gga ctg G u G u Arg G y Leu 195 aac ttc gga cac tgc Asn Phe G y Hi s Cys 210 gca ggc atc cca ctg Ala Gy lie Pro Leu 225 cgt gca Arg Al a gct ttc Al a Phe cga Ar g 245 t agt ct t t aa t ccaagt aag t ac <210> 16 <211> 249 <212> PRT <213> Corynebacter i um gl utam cum <400> 16 IVet Lys Leu Thr Leu u lie Trp Arg On Ala Gy Pro Thr Ala G u 1 5 10 Sy Lys Phe G u Thr Val G n Val Asp Asp Ala Val Ala G n et Ser 25 IIe Leu u Leu Leu Asp His Val Asn Asn Lys Phe lie G u G u G y 40 Lys G u Pro Phe Al a Phe Al a Ser Asp Cys Arg G u G y lie Cys G y 55 Page Thr Cys y Leu Leu Val Asn Gene Sequence 24-2-06.txt Sy Arg Pro His y Ala Asp n Asn Lys Leu bet Tyr Val 145 Gy Phe G u Asn Al a 225 Ar g Al a SIle Val 115 Thr Hi s Gy Gy Ar g 195 Gy l e Al a Al a Pr o Ar g Asn Thr Cys 165 Lys Leu Cys Leu Ar g 245 n Leu Ser Al a Al a 150 Val Leu Ar g Ser Thr 230 Gy Ar g Ar g Al a Gy 135 G u Al a Val Al a Leu 215 Al a Lys Val Al a 105 Asp Al a Al a Cys Leu 185 Lys Gy Al a Asp Ser 90 Al a Ar g Pr o Leu Pr o 170 Ser bet G u Al a Tyr Tyr Val Asp Asp 155 Asn Leu Val Cys Val 235 Lys Pr o Met Al a 140 Hi s Gy Leu Asp Al a 220 Thr G u Val G u 125 Asp Al a Al a Pr o G u 205 Asp Lys Gy I e 110 G n Thr Al a Al a Leu 190 bet Val G u Asp Lys Gy Leu Cys Hi s 175 Gy G u Cys Ar g Thr Asp Gy Hi s SIe 160 Leu Lys Thr Pr o Al a 240 <210> 17 <211> 1530 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1507) <223> RXA01535 <400> 17 acccacctca ctctaggggt ggactccagt gtttcgcgac gacagccgta tttaattctc agtaagaaat gagtgatttc ttc Phe gct Al a tct Ser aag Lys at t SIle tgg Tr p cgt Ar g gct Al a cac Hi s gca Al a ct g Leu gcg Al a gac Asp cag G n gaa G u cag G n acc Thr acc Thr t cc Ser gt a Val at g et cag G n gca Al a 45 aac Asn ggt Sy cgc Ar g cag G n aag Lys gaa gt g G u Val 15 gct gtt Al a Val atc cgc li e Ar g gac t cc Asp Ser Page 21 aacacaatga gtaagcttgt atg acc gag cag gaa et Thr G u G n G u 1 aag gtt cca gca aag Lys Val Pr o Al a Lys gag aac ttc cct atc Su Asn Phe Pro lie gca atg ggt ctg ctg Al a Vet G y Leu Leu ggt gcg ctg gat gca G y Al a Leu Asp Al a Gene Sequence 24-2-06.txt gag G u aag Lys act Thr gct Al a t cc Ser gct Al a 150 ttg Leu acc Thr ggc Gy ctt Leu ggt Gy 230 at c SIle gag G u cgc Ar g at g bet ct g Leu 310 gca Al a gac Asp t cc Ser ggc Gy 120 aat Asn gt c Val aag Lys ct g Leu gct Al a 200 cgc Ar g aac Asn ttg Leu cag G n at c SIle 280 t cc Ser cca Pr o gcc Al a gag G u at g bet gag G u acc Thr gac Asp gct Al a 170 gac Asp cag G n ggt Gy t cc Ser gac Asp 250 gca Al a gt c Val cca Pr o t cc Ser at t l Ie cca Pr o acc Thr cac Hi s cct Pr o 140 at c SIle gag G u gtt Val cag G n ct g Leu 220 gat Asp aag Lys gac Asp ttg Leu acc Thr 300 at c SIle gca Al a at t SIle aat Asn cca Pr o 125 act Thr cca Pr o tgg Tr p cca Pr o ct c Leu 205 gct Al a ttc Phe gag G u gct Al a t ac Tyr 285 ggt Gy at g bet gct Al a gat Asp gag G u 110 aat Asn gca Al a ggc Gy tct Ser gt a Val 190 ggc Gy at t l Ie ggc Gy ct c Leu ctt Leu 270 aag Lys ctt Leu cca Pr o aag Lys ttc Phe at c SIle cac Hi s cac Hi s aag Lys 160 gtt Val ct g Leu gag G u ggc Gy aag Lys 240 gaa G u gag G u gct Al a gag G u aag Lys 320 gag G u cag G n gct Al a gt c Val gtt Val 145 gtt Val gtt Val ggc Gy cgc Ar g acc Thr 225 gtt Val gct Al a ttc Phe aac Asn at c SIle 305 gt c Val at c SIle act Thr t cc Ser aac Asn 130 gct Al a ct g Leu aag Lys cag G n gtt Val 210 gct Al a gtt Val gag G u t cc Ser gat Asp 290 cgt Ar g aac Asn gcg Al a ggt Gy at c SIle 115 at g bet gca Al a cac Hi s t cc Ser gag G u 195 gag G u gct Al a gct Al a aac Asn ggc Gy 275 at c SIle ct c Leu cca Pr o t cc Ser t cc Ser 100 gcg Al a ggt Gy acc Thr gag G u ggc Gy 180 ttc Phe gct Al a ggt Gy gaa G u cac Hi s 260 gca Al a cgc Ar g cca Pr o gtt Val 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 tgt gag acc gct acc cag gtt tcc gct cag gtt Cys G u Thr Ala Thr G n Val Ser Ala G n Val atc ggc aat gac gca I e y Asn Asp Ala Page 22 Gene Sequence 24-2-06.txt 330 335 340 gct gtt gcg ttc tcc ggc acc cag ggc cag ttc gag ctc aac gtg ttc 1171 Ala Val Ala Phe Ser G y Thr G n y G n Phe G u Leu Asn Val Phe 345 350 355 atc cca gtg atg gct cgc aac gtg ctt gag tcc gct cgc ctg ctg gct 1219 lie Pro Val IVet Ala Arg Asn Val Leu u Ser Ala Arg Leu Leu Ala 360 365 370 aac act tcc cgc gtg ttc gca acc cgt ctc gtt gat ggc att gag cca 1267 Asn Thr Ser Arg Val Phe Ala Thr Arg Leu Val Asp y lie G u Pro 375 380 385 aac gag gca cac atg aag gag ctc gct gag tct tca cct tcc atc gtt 1315 Asn G u Ala His IVet Lys G u Leu Ala u Ser Ser Pro Ser lie Val 390 395 400 405 acc cca ctg aac tct gca atc ggc tac gaa gct gct gca aag gtg gct 1363 Thr Pro Leu Asn Ser Al a Ile y Tyr G u Ala Ala Ala Lys Val Ala 410 415 420 aag act gct ttg gct gag ggc aag acc atc cgc cag act gtc atc gat 1411 Lys Thr Ala Leu Ala G u y Lys Thr lie Arg G n Thr Val lie Asp 425 430 435 ttg ggc ttg gtt gat ggc gag aag ctc acc gag gaa gag ctg gac aag 1459 Leu y Leu Val Asp G y G u Lys Leu Thr G u G u G u Leu Asp Lys 440 445 450 cgc ctc gac gtt ctt gct atg gct cac acc gag cgc gag aac aag ttc 1507 Arg Leu Asp Val Leu Al a IVet Ala His Thr G u Arg G u Asn Lys Phe 455 460 465 taaaactaga acccgataaa taa 1530 <210> 18 <211> 469 <212> PRT <213> Corynebacter i um gl ut am cum <400> 18 Net Thr Ou On Ou Phe Arg IIe Ou His Asp Thr Net Gy Ou Val 1 5 10 Lys Val Pro Ala Lys Al a Leu Trp G n Ala G n Thr G n Arg Ala Val 25 Su Asn Phe Pro lie Ser y Arg y Leu u Ser Ala n lie Arg 40 Ala IVet y Leu Leu Lys Ala Ala Cys Ala G n Val Asn Lys Asp Ser 55 G y Al a Leu Asp Al a G u Lys Al a Asp Al a I e I le Ala Ala y Lys 70 75 G u IIe Ala Ser G y Lys His Asp Ala G u Phe Pro IIe Asp Val Phe 90 G n Thr y Ser y Thr Ser Ser Asn Iet Asn Thr Asn G u Val lie 100 105 110 Ala Ser ile Ala Lys Ala Asn y Val u Val His Pro Asn Asp His 115 120 125 Val Asn IVet G y G n Ser Ser Asn Asp Thr Phe Pro Thr Ala Thr His Page 23 Gene Sequence 24-2-06.txt 140 lie Pro Gy Leu Val Al a Al a 145 Val Leu Hi s Val Lys Ser Gy Gn Gu 195 Arg Val G u 210 Thr Al a Al a 225 Val Val Al a Al a u Asn Phe Ser G y 275 Asn Asp Il e 290 SI e Ar g Leu 305 Val Asn Pro I le G y Asn G u Leu Asn 355 Al a Ar g Leu 370 Asp G y Ile 385 Ser Pro Ser Al a Al a Lys G n Thr Val 435 Su G u Leu 450 Arg u Asn 465 <210> 19 <211> 1164 G u Ser 165 Ar g Sy Thr Thr Leu 245 Phe bet Leu Asp Leu 325 Al a Phe Al a Pr o Val 405 Al a Asp Lys Phe Al a 150 Leu Thr Sy Leu Sy 230 I le G u Ar g bet Leu 310 Cys Al a SIle Asn Asn 390 Thr Lys Leu Ar g Al a Al a Hi s Tyr Pr o 215 I le Asn Al a Val Sy 295 G n G u Val Pr o Thr 375 G u Pr o Thr Sy Leu 455 Val Lys Leu Al a 200 Ar g Asn Leu G n SIle 280 Ser Pr o Thr Al a Val 360 Ser Al a Leu Al a Leu 440 Asp Asn Asp Leu 155 Lys Al a Asn 170 IVet Asp Al a 185 Arg n lle Leu G y G u Thr Ser Al a 235 Thr Asp Val 250 Al a Al a Ar g 265 Al a Val Ser G y Pro Leu G y Ser Ser 315 Al a Thr G n 330 Phe Ser G y 345 IVet Al a Arg Arg Val Phe His Vet Lys 395 Asn Ser Al a 410 Leu Al a G u 425 Val Asp G y Val Leu Al a Tr p Pr o Leu 205 Al a Phe G u Al a Tyr 285 Sy vet Ser G n Val 365 Thr Leu Sy Lys Lys 445 Al a Ser Val 190 Gy l e Gy Leu Leu 270 Lys Leu Pr o Al a Sy 350 Leu Ar g Al a Tyr Thr 430 Leu Hi s G u 175 Thr SIe Sy Sy Lys 255 Val SIe Sy Sy G n 335 G n G u Leu G u G u 415 I e Thr Thr Page 24 Gene Sequence 24-2-06.txt <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(1141) <223> RXA00517 <400> 19 ggtcttagaa ccagcgtgca ctgatggcga ttaaaggggg ttgcgcctat acctattgct ggtatacatt tcggtatacc taaaccgaat tgagggattc gt c Val act Thr cag G n tgg Tr p gt g Val aat Asn act Thr aat Asn gga Gy tgg Tr p 150 gca Al a gtt Val cct Pr o gcc Al a gt a Val gat Asp gt g Val gtt Val at c SIle gca Al a ttt Phe 120 gtt Val ggc Gy ttt Phe cgc Ar g act Thr 200 caa G n gca Al a tgg Tr p aga Ar g aat Asn caa G n at g Met act Thr at t SIle gaa G u gaa G u 170 aag Lys gcg Al a ct c Leu gca Al a ggt Gy ct c Leu gat Asp 75 gtt Val tt c Phe gcc Al a ct c Leu aaa Lys 155 acg Thr at c SIle at c l Ie act Thr gac Asp cac Hi s cac Hi s ttg Leu tta Leu ggc G y at g let acc Thr 140 cgg Ar g gct Al a t ac Tyr acg Thr gtt Val gcc Al a ccc Pr o 45 agt Ser ggt G y gct Al a at c SIle t ac Tyr 125 acc Thr at c SIle acg Thr cac Hi s agt Ser 205 ct c Leu cat Hi s t cc Ser ggc G y gcc Al a gat Asp ggt G y 110 t ac Tyr aat Asn ggt Gy gt a Val gca Al a 190 gag G u tgc Cys 15 ctt Leu cac Hi s gcg Al a gt g Val cat Hi s 95 gcg Al a acc Thr gca Al a acc Thr gt c Val 175 cga Ar g ggc Gy aca Thr gt c Val ggt Gy at g tet ttg Leu 80 gct Al a gt g Val cgg Ar g tct Ser aac Asn 160 gat Asp cag G n gca Al a at g Net 1 gac Asp ggt G y gt g Val act Thr gcg Al a cgt Ar g tcg Ser aaa Lys ccg Pr o 145 cca Pr o at a SIle aca Thr ccc Pr o at c SIle gat Asp ctt Leu aca Thr ttg Leu aaa Lys gt g Val gcg Al a 130 gcg Al a tgg Tr p gcc Al a aac Asn acc Thr 210 cca gaa gtc act Pro G u Val Thr ct c Leu agt Ser cga Ar g cat Hi s gat Asp gaa G u cgc Ar g 115 gca Al a at g let tct Ser aat Asn at g let 195 acg Thr acc Thr ttg Leu ct g Leu gca Al a gga G y gca Al a 100 aac Asn gcg Al a gcg Al a at t SIle act Thr 180 ccc Pr o gat Asp aaa Lys gtg Val cct Pr o cac Hi s cac Hi s gtg Val t cc Ser caa G n ccc Pr o gcg Al a 165 gcg Al a att SIe cct Pr o gct gag gca atc aac ggt gtc gtc ctt ccc atg gct ggt cac aaa ggt Page Gene Sequence 24- 2- 06. t xt Al a u Ala lIe Asn y Val Val Leu Pro Met Ala G y His Lys y 215 220 225 tat gcg att agc ttc atg atg gat gtg ctt tct gga gtt ctc act ggt 835 Tyr Ala lie Ser Phe IVet IVet Asp Val Leu Ser G y Val Leu Thr G y 230 235 240 245 tcc cag cac agc acc aag gta cat ggt ccg tat gat ccc act ccc cca 883 Ser G n His Ser Thr Lys Val His Gy Pro Tyr Asp Pro Thr Pro Pro 250 255 260 ggt gga gct ggc cac ttg ttc atc gcg ttg gat gtt gca gcg ttt cgc 931 G y G y Ala G y His Leu Phe lie Ala Leu Asp Val Ala Ala Phe Arg 265 270 275 gat cca caa gat ttc gat gac gca ctc agc gat ctg gtt ggg gaa gtt 979 Asp Pro G n Asp Phe Asp Asp Ala Leu Ser Asp Leu Val Gy G u Val 280 285 290 aaa tcc act ccg aaa gca caa aac acc gag gag att ttc tac ccc ggt 1027 Lys Ser Thr Pro Lys Al a G n Asn Thr G u G u lie Phe Tyr Pro y 295 300 305 gaa tcg gaa gac cgt gcg cat cgg aaa aac tct gcg cac ggt att tca 1075 G u Ser G u Asp Arg Al a His Arg Lys Asn Ser Ala His G y lie Ser 310 315 320 325 ttg cct gaa aaa acg tgg atg gaa ctg caa gaa ctg gca atc gag aac 1123 Leu Pro u Lys Thr Trp Iet G u Leu Gn u Leu Ala lie u Asn 330 335 340 cat gtt gta act cac cgt tgatctgcgc gttaaacctg gcc 1164 His Val Val Thr His Arg 345 <210> <211> 347 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> IVet Pro u Val Thr Val Asn Ala n n Leu Thr Val Leu Cys Thr 1 5 10 Asp Ile Leu Thr Lys Thr y Val Pro Ala Ala Asp Ala His Leu Val 25 G y Asp Ser Leu Val G n Ala Asp Leu Trp Gy His Pro Ser His G y 40 Val Leu Arg Leu Pro Trp Tyr Val Arg Arg Leu His Ser G y Ala IVet 55 Thr Thr His Ala His Val G u Val Leu Asn Asp Leu y Ala Val Leu 70 75 Ala Leu Asp y His Asn y lie G y n Val Leu Ala Asp His Ala 90 Arg Lys u Ala Val Thr Arg Ala IVet IVet Phe Gy Ile y Ala Val 100 105 110 Ser Val Arg Asn Ser Asn His Phe Gy Thr Ala IVet Tyr Tyr Thr Arg 115 120 125 Lys Ala Ala Ala n G y Cys Val Ser lie Leu Thr Thr Asn Ala Ser 130 135 140 Page 26 Gene Sequence 24-2-06.txt Pro Ala IVet Ala Pro 145 Pr o SIle Thr Pr o Al a 225 Gy Asp Val Leu SIle 305 Al a Leu Ser Asn bet 195 Thr Hi s Leu Thr Al a 275 Gy Tyr Gy lie Al a 165 Al a SIle Pr o Gy Gy 245 Pr o Ar g Val Gy Ser 325 Asn Tr p 150 Al a Val Pr o Al a Ty r 230 Ser Gy Asp Lys G u 310 Leu Hi s Gy Gy Arg u Pr o Al a G u G u 215 Al a G n Gy Pr o Ser 295 Ser Pr o Val Phe Ar g Thr 200 Al a l Ie Hi s Al a G n 280 Thr G u G u Val Gy Gy 185 Tr p l Ie Ser Ser Gy 265 Asp Pr o Asp Lys Thr 345 Lys 155 Thr SIle SIle Gy bet 235 Lys Leu Asp Al a Al a 315 Tr p Ar g Al a Tyr Thr Val 220 Met Val Phe Asp G n 300 Hi s bet Thr Hi s Ser 205 Val Asp Hi s SIle Al a 285 Asn Ar g G u Val Al a 190 G u Leu Val Gy Al a 270 Leu Thr Lys Leu Arg lie y Thr Asn Val 175 Ar g Gy Pr o Leu Pr o 255 Leu Ser G u Asn G n 335 <210> 21 <211> 1107 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1084) <223> RXA01350 <400> 21 tgcagtatcg tcaagatcac ccaaaactgg tggctgttct ggttcttaga ggacccccta caaggattga ggattgttta aac gtc tcc acc aag Asn Val Ser Thr Lys tct tat tca ctg ttg Ser Tyr Ser Leu Leu gac acc cct gta gaa Asp Thr Pro Val G u aag gtc acc gtc acc ggc Lys Val Thr Val Thr G y 15 tgg cgc atc gcc aac ggt Trp Arg lie Ala Asn y 30 ctg aaa ctt ctg gag atc Leu Lys Leu Leu G u Ile Page 27 cttttaagcg ggatagcatg atg aat tcc ccg cag 115 IVet Asn Ser Pro G n 1 gca gct ggt caa atc 163 Ala Ala y G n Ile gaa gta ttc ggc acc 211 G u Val Phe y Thr cct cag gct ctt ggc 259 Pro G n Ala Leu G y Gene Sequence 24-2-06.txt 45 ggg gca gag ggt gtg gct atg gaa ctt ctg gat tct gcc ttc ccc ctc 307 Gy Ala G u y Val Al a Iet G u Leu Leu Asp Ser Ala Phe Pro Leu 60 ctg cga aac atc acc atc acc gcg gat gcc aat gag gca ttc gac ggc 355 Leu Arg Asn Ile Thr I e Thr Ala Asp Ala Asn G u Ala Phe Asp y 75 80 gct aat gcg gcg ttt ttg gtc ggt gcg aag cct cgc gga aaa ggc gaa 403 Ala Asn Ala Ala Phe Leu Val Gy Ala Lys Pro Arg y Lys Gy G u 95 100 gag cgc gca gat ttg ctg gct aac aac ggc aag att ttc gga cct caa 451 Su Arg Ala Asp Leu Leu Ala Asn Asn y Lys Ile Phe y Pro n 105 110 115 ggt aaa gct atc aat gac aac gcc gca gat gac att cgt gtc cta gtt 499 Sy Lys Ala lie Asn Asp Asn Ala Ala Asp Asp ile Arg Val Leu Val 120 125 130 gtt gga aac cca gcg aac acc aac gcg ttg att gct tca gct gcg gcc 547 Val y Asn Pro Ala Asn Thr Asn Ala Leu Ile Ala Ser Ala Ala Ala 135 140 145 cca gat gtt cca gca tcc cgc ttc aac gca atg atg cgc ctt gat cac 595 Pro Asp Val Pro Ala Ser Arg Phe Asn Ala IVet IVet Arg Leu Asp His 150 155 160 165 aac cgt gcg atc tcc cag ctg gcc acc aag ctt ggc cgt gga tct gcg 643 Asn Arg Ala lie Ser G n Leu Ala Thr Lys Leu y Arg y Ser Ala 170 175 180 gaa ttt aac aac att gtg gtc tgg gga aat cac tcc gca acc cag ttc 691 G u Phe Asn Asn lie Val Val Trp G y Asn His Ser Ala Thr G n Phe 185 190 195 cca gac atc acc tac gca acc gtt ggt gga gaa aag gtc act gac ctg 739 Pro Asp Ile Thr Tyr Al a Thr Val Gy Gy G u Lys Val Thr Asp Leu 200 205 210 gtt gat cac gat tgg tat gtg gag gag ttc att cct cgc gtg gct aac 787 Val Asp His Asp Trp Tyr Val G u G u Phe Ile Pro Arg Val Ala Asn 215 220 225 cgt ggc gct gaa atc att gag gtc cgt gga aag tct tct gca gct tct 835 Arg Gy Ala u Ile Ile u Val Arg y Lys Ser Ser Ala Ala Ser 230 235 240 245 gca gca tcc tct gcg att gat cac atg cgc gat tgg gta cag ggc acc 883 Ala Ala Ser Ser Ala lIe Asp His IVet Arg Asp Trp Val G n y Thr 250 255 260 gag gcg tgg tcc tct gcg gca att cct tcc acc ggt gca tac ggc att 931 G u Ala Trp Ser Ser Al a Ala lie Pro Ser Thr Gy Ala Tyr Gy lie 265 270 275 cct gag ggc att ttt gtc ggt ctg cca acc gta tcc cgc aac ggt gag 979 Pro G u Gy Ile Phe Val y Leu Pro Thr Val Ser Arg Asn Gy G u 280 285 290 tgg gaa atc gtt gaa ggc ctg gag att tcc gat ttc cag cgc gcc cgc 1027 Trp u Ile Val G u y Leu u Ile Ser Asp Phe n Arg Ala Arg 295 300 305 atc gac gcg aat gct cag gaa ttg cag gcc gag cgc gag gca gtg cgc 1075 Ile Asp Ala Asn Ala On u Leu G n Ala u Arg u Ala Val Arg Page 28 Gene Sequence 24-2-06.txt 310 315 320 gac ttg ctc taatctttaa cgcatgactt cgc Asp Leu Leu <210> 22 <211> 328 <212> PRT <213> Corynebacter i um gl ut ai cum 1107 <400> 22 IVet Asn Ser 1 Al a Al a G y G u Val Phe Pro G n Ala Ser Al a Phe G u Al a Phe Arg G y Lys lie Phe G y 115 li e Arg Val 130 Al a Ser Al a 145 IVet Arg Leu G y Arg G y Ser Al a Thr 195 Lys Val Thr 210 Pro Arg Val 225 Ser Ser Al a Trp Val n G y Al a Tyr 275 Pro n Asn Val 5 I e Thr Gy Leu Gy G u G n Val Al a Hi s 165 Al a Phe Leu Asn Ser 245 Thr SIle Ser Asp Sy Leu 70 Al a G u Sy Val Pr o 150 Asn G u Pr o Val Ar g 230 Al a G u Pr o Tyr Thr Al a 55 Ar g Asn Ar g Lys Sy 135 Asp Ar g Phe Asp Asp 215 Sy Al a Al a G u Ser Thr Lys 10 Ser Leu Leu Pro Val u 40 G u G y Val Asn Ile Thr Al a Al a Phe 90 Al a Asp Leu 105 Al a lie Asn 120 Asn Pro Al a Val Pro Al a Al a i e Ser 170 Asn Asn Ile 185 Ile Thr Tyr 200 Hi s Asp Tr p Ala G u Ile Ser Ser Al a 250 Trp Ser Ser 265 G y Ile Phe 280 Lys Tr p Leu Al a I e Leu Leu Asp Asn Ser 155 G n Val Al a Tyr SIle 235 l le Al a Val Val Ar g Lys vet Thr Val Al a Asn Thr 140 Ar g Leu Val Thr Val 220 G u Asp Al a Sy Thr SIle Leu G u Al a Sy Asn Al a 125 Asn Phe Al a Tr p Val 205 G u Val Hi s l Ie Leu 285 Val Al a Leu Leu Asp Al a Asn 110 Al a Al a Asn Thr Sy 190 Sy G u Ar g et Pr o 270 Pr o Thr Asn G u Leu Al a Lys Sy Asp Leu Al a Lys 175 Asn Sy Phe Sy Ar g 255 Ser Thr Ser Arg Asn y u Trp u Ile Val u y Leu u lie Ser Asp Page 29 Gene Sequence 24-2-06.txt 290 295 300 Phe Gn Arg Ala Arg lie Asp Ala Asn Ala Gn G u Leu Gn Ala G u 305 310 315 320 Arg G u Ala Val Arg Asp Leu Leu 325 <210> 23 <211> 1092 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1069) <223> RXA02149 <400> 23 ttgcagagtg aaccacgatg atggttggac agctgttgat aat t caccta aatcctgtgt agaacgcgag gggcactct t agctaatctt tgaaagatta gcc Al a ggg Sy tcg Ser gaa G u gcg Al a ctt Leu ggc Sy gag G u gca Al a t ac Tyr 150 ttc Phe gt c Val cgc Ar g ct c Leu ttt Phe tgg Tr p cct Pr o cgt Ar g 120 ggc Sy ggt Sy gt g Val gaa G u acg Thr gcc Al a gat Asp gat Asp cgt Ar g ggt Sy gga Sy t at Tyr ttt Phe ggg G y gca Al a t ac Tyr gag G u cca Pr o gaa G u gct Al a ggt Sy 140 acg Thr gac Asp cgc Ar g at g Met 45 ccg Pr o tgc Cys gt g Val cat Hi s caa G n 125 gca Al a gca Al a at c SIle at c I e gaa G u gtt Val gag G u cgt Ar g gat Asp 110 ggc Sy gcg Al a cca Pr o ggc G y 15 gt g Val cag G n ggt G y gtt Val gaa G u 95 gcc Al a gct Al a ct g Leu gaa G u ggc G y acc Thr ggg G y gct Al a gtt Val aag Lys aac Asn gac Asp at t SIle ttt Phe 160 at g Met 1 acc Thr agt Ser at t SIle gt g Val cga Ar g ttg Leu t ca Ser aac Asn gaa G u 145 ggt Sy aac Asn ttg Leu ttt Phe gga G y ttt Phe gaa G u gca Al a tgg Tr p 130 aaa Lys cat Hi s cca caa aaa Pro G n Lys cga Ar g gcg Al a ct a Leu gcc Al a ccg Pr o ctt Leu 100 tgg Tr p ttg Leu gaa G u cgt Ar g gtt cgt ggc gga cgc gca tgt gcg tgt ggc Val Arg Gy Gy Arg Ala Cys Ala Cys y aaa gaa ggc tgc ctg gag Lys G u G y Cys Leu G u Page Gene Sequence 24-2-06.txt 170 175 180 cgt tac tgt tcc ggt act gcc ttg gtt tac act gcg cgt gaa ttg gct 691 Arg Tyr Cys Ser y Thr Ala Leu Val Tyr Thr Ala Arg G u Leu Ala 185 190 195 tcg cat ggc tca ttc cgc aac agc ggg ctg ttt gac aag atc aaa gcc 739 Ser His G y Ser Phe Arg Asn Ser G y Leu Phe Asp Lys IIe Lys Ala 200 205 210 gat ccg aat tcc atc aat gga aaa acg atc act gcg gca gcg cgc caa 787 Asp Pro Asn Ser Ile Asn y Lys Thr Ile Thr Ala Ala Ala Arg n 215 220 225 gaa gac cca ctt gct ctc gcc gtt ctg gaa gat ttc agc gag tgg ctg 835 G u Asp Pro Leu Ala Leu Ala Val Leu G u Asp Phe Ser G u Trp Leu 230 235 240 245 ggc gaa act ttg gcg atc att gct gat gtc ctt gac cca ggc atg atc 883 Sy G u Thr Leu Ala lie ile Ala Asp Val Leu Asp Pro Gy IVet Ile 250 255 260 atc att ggt ggc gga ctg tcc aat gct gcc gac ctt tat ttg gat cgc 931 lie lie Gy Gy y Leu Ser Asn Ala Ala Asp Leu Tyr Leu Asp Arg 265 270 275 tcg gtc aac cac tat tcc acc cgc atc gtc ggc gca gga tat cgc cct 979 Ser Val Asn His Tyr Ser Thr Arg IIe Val G y Ala G y Tyr Arg Pro 280 285 290 ttg gca cgc gtt gcc aca gct cag ttg ggt gcg gat gct ggc atg atc 1027 Leu Ala Arg Val Al a Thr Al a n Leu G y Ala Asp Al a y IVet Ile 295 300 305 ggt gtc gct gat cta gct cga cgc tct gta gtg gaa gcc aac 1069 Sy Val Ala Asp Leu Al a Arg Arg Ser Val Val G u Ala Asn 310 315 320 taggtgtttt tcggtgggct gcg 1092 <210> 24 <211> 323 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 24 IVet Pro n Lys Pro Ala Ser Phe Ala Val Gy Phe Asp lie Gy Gy 1 5 10 Thr Asn IVet Arg Ala G y Leu Val Asp u Ser Gy Arg Ile Val Thr 25 Ser Leu Ser Ala Pro Ser Pro Arg Thr Thr G n Ala Iet G u G n y 40 Ile Phe Asp Leu Val G u G n Leu Lys Ala G u Tyr Pro Val y Ala 55 Val y Leu Ala Val Al a y Phe Leu Asp Pro G u Cys G u Val Val 70 75 Arg Phe Ala Pro His Leu Pro Trp Arg Asp G u Pro Val Arg G u Lys 90 Leu G u Asn Leu Leu G y Leu Pro Val Arg Leu G u His Asp Ala Asn 100 105 110 Page 31 Ser Ala Ala Trp Gy G u His 115 Gene Sequence 24-2-06.txt Arg Phe Gy Ala Ala G n Gy Ala Asp 120 125 Asn G u 145 Gy G u Al a Asp Al a 225 Phe Asp Leu Al a Asp 305 Gu Val Leu Leu Al a G y G u Ile Tyr 150 Leu Arg Val Val 165 Cys Leu G u Arg 180 G u Leu Ala Ser 195 Ile Lys Ala Asp Al a Arg G n G u 230 G u Trp Leu G y 245 G y IV t Ile lle 260 Leu Asp Arg Ser 275 Tyr Arg Pro Leu G y lVt Ile G y 310 Asn Leu 135 Ar g Ar g Tyr Hi s Pr o 215 Asp G u SIle Val Al a 295 Thr Al a Gy Ser 185 Ser Ser Leu Leu Gy 265 Hi s Val Gy Tyr Ar g 170 Gy Phe SIle Al a Al a 250 Gy Tyr Al a SIle Gy 155 Al a Thr Ar g Asn Leu 235 l le Leu Ser Thr Al a 315 Gy 140 Thr Cys Al a Asn Gy 220 Al a SIle Ser Thr Al a 300 Al a Al a Al a Leu Ser 205 Lys Val Al a Asn Ar g 285 G n Al a Pr o Cys Val 190 Gy Thr Leu Asp Al a 270 I le Leu Leu G u Gy 175 Tyr Leu SIe G u Val 255 Al a Val Gy Val Ala Asp Leu Arg Arg Ser Val <210> <211> 1785 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1762) <223> RXA01814 <400> tgttaagcca ccctactccg tgaattttgc cgtatctcgt gcgcacaatt gcttttgagg ggaagatgaa gagaaagtat tggtgtttta aggagcaaac atg gca cat gaa cgc lVbt Ala His G u Arg 1 gcc ggg caa ctc gcc caa cca gaa gat ctc atc gat gtt gcg gaa ctg Al a Gy n Leu Al a n Pro Gu Asp Leu Ile Asp Val Ala u Leu 15 gtc acc gca tat ttc acc cgc aag ccg gac gtg aac aac cct gat cag Val Thr Ala Tyr Phe Thr Arg Lys Pro Asp Val Asn Asn Pro Asp G n 30 Page 32 Gene Sequence 24- 2- 06. txt cag gtc gct ttc ggc acc tcc gga cac cgt ggc ttc gcg ctg gac agc G n Val Ala Phe G y Thr Ser G y His Arg G y Phe Ala Leu Asp Ser gct Al a t ac Tyr cgc Ar g gt c Val t ac Tyr ggc Gy at c SIle 150 t ac Tyr at c SIle aag Lys gac Asp gat Asp 230 ggc Gy aac Asn aca Thr gca Al a aac Asn aac Asn acg Thr at t SIle ccg Pr o 120 at c SIle at c SIle cca Pr o aac Asn gtt Val 200 aag Lys at c SIle t cc Ser acc Thr gac Asp 280 gca Al a gac Asp cag G n gcg Al a aac Asn ccc Pr o ggc Gy cca Pr o aac Asn 170 gcc Al a gtt Val at t SIle gaa G u gat Asp 250 gt c Val gac Asp ct g Leu cac Hi s cca Pr o 75 ct g Leu gac Asp gca Al a acc Thr t cc Ser 155 ggt Gy aac Asn t cc Ser tac Ty r gct Al a 235 tac Ty r aac Asn ggc Gy at t SIle at c I e aaa Lys t cc Ser gt c Val gt g Val gca Al a 140 cac Hi s ggc Gy gat Asp ggt Gy at c SIle 220 ggt Gy tgg Tr p cca Pr o aag Lys gac Asp 300 ct g Leu aac Asn gaa G u gaa G u t cc Ser 125 gga Gy aac Asn cct Pr o at t SIle gt c Val 205 gct Al a gtt Val ggt Gy cac Hi s at c SIle 285 aac Asn gca Al a tgg Tr p cca Pr o gt g Val 110 cac Hi s ccc Pr o cct Pr o gca Al a ct g Leu 190 ct c Leu gac Asp cga Ar g gcc Al a gtt Val 270 cgc Ar g cga Ar g acc Thr gt c Val gcg Al a 95 ctt Leu gcg Al a t cc Ser cct Pr o gat Asp 175 cgc Ar g gac Asp ct g Leu at c SIle at c l Ie 255 gat Asp at g let gac Asp acc Thr ggc Gy at g let gt c Val at c l Ie cgc Ar g cgt Ar g 160 acc Thr ggc Gy gag G u cca Pr o ggc Gy 240 gca Al a t cc Ser gac Asp aag Lys cag G n ccg Pr o at c SIle gac Asp ct a Leu ccc Pr o 145 gat Asp gac Asp gac Asp cgc Ar g aac Asn 225 gca Al a gaa G u acc Thr tgc Cys ttc Phe 305 gcg Al a ct g Leu agc Ser gcc Al a cga Ar g 130 t ac Tyr ggc Gy gcc Al a ctt Leu acc Thr 210 gt g Val gac Asp acc Thr ttc Phe t cc Ser 290 gat Asp at c l Ie ttt Phe gcg Al a gac Asp 115 cac Hi s gcc Al a gga Gy acc Thr gca Al a 195 act Thr gt c Val cca Pr o cat Hi s cgc Ar g 275 agc Ser gt g Val gt c Val at c SIe ctt Leu 100 ggc Gy aac Asn gac Asp ttc Phe gac Asp 180 gac Asp gcc Al a aac Asn at g Net ggc Gy 260 ttc Phe cca Pr o gca Al a 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 Page 33 ggc aac gc G y Asn A 310 ggc ttg at G y Leu M ttt gct c Phe Al a Hi ct g gt c ac Leu Val S 3( acc ct c gt Thr Leu V, 375 atc tcc g( Ile Ser G 390 ttc ctc c( Phe Leu Ar atc ctt gc Ile Leu A, cca t ca c Pro Ser G 4L tac gcc cc Tyr Al a Ar 455 aag gca ct Lys Ala LE 470 gca at c ac Al a I e T gga gga ct G y G y LE t cc ggc ac Ser G y T gaa gag c Su G u Hi 535 gaa gt a ct G u Val LE 550 <210> 26 <211> 554 <212> PRT Gene Sequence 24-2-06.txt ac gcc gac gcc gac cgc cac ggc atc gtc acc cca gac gct ;p Ala Asp Ala Asp Arg His y lle Val Thr Pro Asp Ala g aac ccc t Asn Pro 330 ac cgc cca s Arg Pro 345 gc t cc t cc er Ser Ser 30 t gag gtt al G u Val gc gaa at c y G u lle gc atg gac g IVet Asp 410 ac ct c ct g sp Leu Leu 425 ag cgc tac n Arg Tyr 40 gc acc gat rg Thr Asp g tcc cca eu Ser Pro cc gct aag hr Al a Lys 490 a aaa gt g eu Lys Val 505 cc gaa gac hr G u Asp 20 ac ct c gcc s Leu Al a c gga cag 3u 1 y G n 315 aac Asn ggt G y at g vbet cca Pr o gga G y 395 ggc Gy gca Al a gca Al a gca Al a gaa G u 475 ct c Leu acc Thr aag Lys cag G n cac Hi s tgg Tr p at c SI e gt c Val 380 ttc Phe acc Thr gct Al a gaa G u gaa G u 460 cag G n acc Thr acc Thr t ac Tyr gtt Val 540 t ac ct c gca Tyr Leu Al a 335 t cc gca gat Ser Al a Asp 350 gac cgc gtt Asp Arg Val 365 gga ttc aag G y Phe Lys ggt ggt gaa Sy y u acc t gg t cc Thr Trp Ser 415 gag atc at t G u lie lle 430 ctc gcc gaa Leu Al a G u 445 gcc aac cga Al a Asn Ar g gtc acc gcc Val Thr Al a gaa gct ccc G u Ala Pro 495 gaa aac gcc G u Asn Al a 510 aag at c t ac Lys Ile Tyr 525 cag aag gaa G n Lys u 320 gt a Val acc Thr gt g Val tgg Tr p gaa G u 400 acc Thr gca Al a gaa G u gaa G u acc Thr 480 ggc Sy tgg Tr p gca Al a gcc Al a gca Al a gca Al a gcg Al a ttt Phe 385 t cc Ser gac Asp gt a Val ttc Phe caa G n 465 gaa G u aat Asn ttc Phe gaa G u caa G n 545 at t l Ie gt g Val cag G n 370 gt c Val gca Al a aag Lys acc Thr ggt Sy 450 aag Lys ct a Leu ggc Sy gca Al a t cc Ser 530 gcg Al a gag G u ggc G y 355 ctt Leu cca Pr o ggt G y gac Asp ggc Sy 435 gca Al a gcc Al a gcc Al a gca Al a gca Al a 515 ttc Phe ttg Leu t ac Tyr 340 aaa Lys ggc G y ggt G y gca Al a ggc Gy 420 aag Lys cct Pr o at c SIe ggc Sy gcc Al a 500 cgc Ar g aag Lys gt c Val 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1785 taaaactgcg gacttgctga caa Page 34 Gene Sequence 24-2-06.txt <213> Corynebacter i um gl ut ai cum <400> 26 let Ala His G u 1 Asp Asn Phe G n Pr o SIle Asp Leu Pr o 145 Asp Asp Asp Ar g Asn 225 Al a G u Thr Cys Phe 305 Val Al a Pr o Leu SIle Phe Al a Asp 115 Hi s Al a Gy Thr Al a 195 Thr Val Pr o Hi s Ar g 275 Ser Val Pr o Ar g 5 Leu G n Ser Asp Gy G u Ar g Asp Gy Lys 165 Tr p Val Tyr SIle Gy 245 Leu let Hi s Thr Al a 325 Val G n Al a Ty r 70 Ar g Val Ty r Gy SIle 150 Ty r SIle Lys Asp Asp 230 Gy Asn Thr Al a Gy 310 Gy Thr Val Phe Ar g Asp Leu Thr SIle 135 Val Asn Al a Ar g Phe 215 Al a Al a Leu Leu let 295 Asn Leu Al a G y n Leu Al a Al a 40 Asn Asn Thr SIle Pr o 120 I e I e Pr o Asn Val 200 Lys l Ie Ser Thr Asp 280 Al a Asp let Tyr Phe G u G n Hi s Al a 105 Thr Leu Thr Al a Ar g 185 Pr o Gy Ar g Val Val 265 Thr Ser Al a Asn Ar g 345 Al a 10 Phe Gy Asp G n Al a 90 Asn Pr o Gy Pr o Asn 170 Al a Val SIle G u Asp 250 Val Asp Leu Asp Pr o 330 G n Thr Thr Hi s Pr o Leu Asp Al a Thr Ser 155 Sy Asn Ser Tyr Al a 235 Tyr Asn Sy SIle Al a 315 Asn G u Lys Sy Leu Asn G u G u Ser 125 Sy Asn Pr o SIle Val 205 Al a Val Sy Hi s SIle 285 Asn Ar g Tyr Leu Asp Ar g Thr Val Al a Leu Al a Ser Pr o Asp 175 Ar g Asp Leu SIe SIe 255 Asp bet Asp Sy Al a 335 Al a ll e G u Leu Phe Ala Hi s Pro Gy Trp Ser Ala Asp Thr 350 Page Gene Sequence 24-2-06.txt Ala Val y 355 Al a G n Leu 370 Phe Val Pro 385 Ser Al a G y Asp Lys Asp Val Thr G y 435 Phe G y Al a 450 G n Lys Al a 465 G u Leu Al a Asn G y Al a Phe Al a Al a 515 G u Ser Phe 530 G n Al a Leu 545 Lys Thr Leu Val Ser Ser Ser Vet lie Asp Arg Val Val 360 365 Ar g Leu Ser 405 Leu Thr Al a Leu G u 485 l le Pr o Sy Thr SIle 390 Phe SIle Pr o Ty r Lys 470 Al a Sy Ser G u Leu 375 Ser Leu Leu Ser Al a 455 Al a l Ie Sy Sy G u 535 G u G u Met Leu 425 Ar g Thr Ser Al a Lys 505 G u Leu Pr o Sy 395 Sy Al a Al a Al a G u 475 Leu Thr Lys G n Val 380 Phe Thr Al a G u G u 460 G n Thr Thr Tyr Val 540 Sy Sy Thr G u Leu 445 Al a Val G u G u Lys 525 G n Phe Sy Tr p SIle 430 Al a Asn Thr Al a Asn 510 I le Lys Lys G u Ser 415 I le G u Ar g Al a Pr o 495 Al a Tyr G u Tr p G u 400 Thr Al a G u G u Thr 480 Gy Tr p Al a Al a Val Ser G u 550 Val Leu y G n <210> 27 <211> 680 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> <223> RXN02803 <400> 27 gtc tct gga gag atg ctc gcg gca gca Val Ser G y G u Met Leu Al a Al a Al a 1 5 cag ggt gtt gat gtc att cgt gtt ggt G n y Val Asp Val I e Arg Val y 25 gca ttc ctc acc gat gat tat ggc gct Al a Phe Leu Thr Asp Asp Tyr G y Al a 40 gca tcc cac aac cca atg ccg gac aac Ala Ser His Asn Pro IVet Pro Asp Asn 55 ctt tca gca ggc atg gcc agc Leu Ser Al a G y lbt Al a Ser 10 gtc atc cca acc cca gct gtt Val Ile Pro Thr Pro Ala Val gac atg ggc gtg atg att tct Asp lVbt y Val lVbt Ile Ser gga atc aag ttc ttt tct gca G y Ile Lys Phe Phe Ser Al a Page 36 Gene Sequence 24-2-06.txt ggt Gy at g Mbt gt c Val aag Lys gca Al a gcg Al a 145 at c l Ie gt c Val gac Asp gga cac aag ctt cca gac G y His Lys Leu Pro Asp cat gtg gaa gac gag att gag cgt gtt His Val Gu Asp Gu Ile Gu Arg Val 75 agc Ser gaa G u gct Al a 115 aat Asn gca Al a at g Met aag Lys tgt Cys 195 cca Pr o gca Al a cct Pr o gcg Al a gt a Val tgc Cys 165 ggt Gy gct Al a 70 gca Al a acc Thr acg Thr gca Al a at t SIle 150 ggt Gy gct Al a gt g Val gaa G u gat Asp t ca Ser agt Ser 135 gct Al a t cc Ser gac Asp aac Asn ggc Gy gca Al a ctt Leu 120 gtt Val at t l Ie acc Thr ctt Leu aag Lys 200 cca Pr o cag G n 105 gaa Gu gt a Val cat Hi s cac Hi s gga Gy 185 gat Asp aca Thr 90 gac Asp ggc Gy gct Al a aac Asn at t SIle 170 ct c Leu gcc Al a ggg Gy cgt Ar g at c SIle cca Pr o aag Lys 155 gat Asp gcg Al a aac Asn gga Gy ct a Leu at t SIle 125 gct Al a gac Asp gcg Al a gac Asp gtt Val 205 ggc Gy cac Hi s gt g Val gag Gu t ac Tyr ccg Pr o 175 gat Asp ggt Gy cgt Ar g ctg Leu gat Asp gct Al a aac Asn 160 cca Pr o gct Al a gac Asp caa atc atg gcg ctg tta gcc att gcg Sn Ile IVet Ala Leu Leu Ala lie Ala 210 215 gaa <210> 28 <211> 219 <212> PRT <213> Corynebact eri um gl ut am cum <400> 28 Val Ser G y G u Net Leu Al a Al a Al a 1 5 G n y Val Asp Val I le Arg Val y Al a Phe Leu Thr Asp Asp Tyr G y Al a 40 Ala Ser His Asn Pro IVet Pro Asp Asn 55 G y G y His Lys Leu Pro Asp His Val 70 IVet Asp Ser Leu Pro Al a u G y Pro Val I le u u Ala Thr Asp Al a n atg aaa IVet Lys Leu Ser 10 Val lile Asp IVet G y lle Su Asp 75 Thr G y 90 Asp Arg Page 37 taaaacggcg agctgcgcaa 677 680 Al a Pr o Gy Lys G u Hi s Tyr Gy Thr Val Phe SIle Gy Leu Al a Al a SIle Ser Ar g Gy Hi s Ser Val Ser Al a Val Ar g Leu 100 Lys G u Ala Val Pro Thr Ser 115 Al a Al a Asn G y Al a Al a Ser 130 135 Ala Gy Ala Thr Val I le Ala 145 150 I e Asn IVet Asp Cys G y Ser 165 Val Leu Lys His G y Al a Asp 180 Asp Arg Cys Leu Ala Val Asn 195 G n i e IVet Ala Leu Leu Ala 210 215 <210> 29 <211> 399 <212> DNA <213> Corynebacter i um gl utar Gene Sequence 24- 2- 06. t xt 105 110 Leu Gu Gy Ile Lys Ile Val 120 125 Val Val Ala Pro Thr Ala Tyr 140 lie His Asn Lys Pro Asp Ser 155 Thr His lie Asp n Ala n 170 Leu G y Leu Al a Hi s Asp G y 185 190 Lys Asp Ala Asn Leu Val Asp 200 205 l e Ala IVet Lys Val G u Tyr Pr o 175 Asp Sy Asp Al a Asn 160 Pr o Al a Asp <220> <221> CDS <222> <223> FRXA02803 <400> 29 tct Ser 1 ggt Sy ttc Phe t cc Ser gga G y gac Asp at c SIle gaa G u gag G u gat Asp acc Thr aac Asn aag Lys ttg Leu gaa G u gtt Val 115 ct c Leu 5 at t SIle gat Asp at g Met cca Pr o gca Al a acc Thr acg Thr gcg Al a cgt Ar g t at Tyr ccg Pr o gac Asp 70 gaa G u gat Asp t ca Ser gca Al a gtt Val ggc G y gac Asp cat Hi s ggc G y gca Al a ctt Leu gca Al a ggt G y gct Al a 40 aac Asn gt g Val cca Pr o cag G n gaa 10u 120 ctt Leu gt c Val gac Asp gga G y gaa G u aca Thr gac Asp 105 ggc G y t ca Ser 10 at c SIle at g Met at c SIle gac Asp ggg G y 90 cgc Ar g at c SIle gca Al a cca Pr o ggc G y aag Lys gag G u 75 cat Hi s t ac Tyr aag Lys ggc G y acc Thr gt g Val ttc Phe att SIle gga G y ct a Leu att SIle at g Met cca Pr o at g Met ttt Phe gag G u gtt Val gag G u gtt Val 125 gcc Al a gct Al a att SIle tct Ser cgt Ar g ggc G y cac Hi s 110 gt g Val agc Ser gtt Val tct Ser gca Al a gtt Val cgt Ar g ct g Leu gat Asp cag G n gca Al a gca Al a ggt G y at g Met gtc Val aag Lys gca Al a gcc aat ggt gcg gca Page 38 Gene Sequence 24-2-06.txt Al a Asn G y Al a Al a 130 <210> <211> 133 <212> PRT <213> Corynebacter i um gl ut ai cum <400> Ser Gy Gu Met Leu Ala Ala Ala Leu Ser Ala 1 5 10 Sy Val Asp Val I e Arg Val y Val Ile Pro 25 Phe Leu Thr Asp Asp Tyr y Ala Asp IVet y 40 Ser His Asn Pro IVet Pro Asp Asn y I I e Lys G y His Lys Leu Pro Asp His Val G u Asp G u 70 75 Asp Ser Leu Pro Al a G u G y Pro Thr G y Hi s 90 lie G u G u Ala Thr Asp Ala n Asp Arg Tyr 100 105 G u Ala Val Pro Thr Ser Leu G u y Ile Lys 115 120 Al a Asn y Al a Al a 130 <210> 31 <211> 1713 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(1690) <223> RXN03076 <400> 31 aatacctttc tgttttgtcc gcaggcgtat caggaaaacc tct aatagtg atcccacgaa aacaaaggat cggggtgttc bet Pr o bet Phe G u Val G u Val 125 Al a Al a I le Ser Ar g Gy Hi s 110 Val Ser Val Ser Al a Val Ar g Leu Asp G n Al a Al a Sy Met Val Lys Al a cag G n cgc Ar g gct Al a gtt agt Ser cag G n tgg Tr p gag ggc G y aat Asn gca Al a gaa aca Thr gtt Val gaa aau aca gca Al a ttg Leu cgt Ar g gga ggg G y cag G n gcg Al a 45 at c ttg Leu gt a Val gca Al a ggc cgt gca Arg Ala 15 acc aga Thr Arg ct a aat Leu Asn agg gcg Page 39 tgcagcgcgg tagagtcgag atg gac gag tct cgt IVet Asp G u Ser Arg 1 cca gtt ggc ccg gcg Pro Val y Pro Ala act aca gca ggt gtt Thr Thr Al a y Val cca gtg ccg cat ttg Pro Val Pro His Leu ttg tat ccc caa gat Val Pro G u Asp G u Thr ggt Sy act Thr gt g Val gt g Val aat Asn cag G n 150 gt g Val gat Asp gat Asp gt g Val gct Al a 230 cag G n gag G u gct Al a ggt Gy gt g Val 310 ttg Leu gct Al a ttg Leu aag Lys 120 gcg Al a t at Tyr gat Asp ct g Leu gct Al a 200 t at Tyr caa G n cct Pr o tct Ser at t l Ie 280 cgt Ar g aca Thr gt c Val acc Thr ccc Pr o ggg Gy gac Asp gaa G u at t SIle 170 cga Ar g ttg Leu gct Al a gct Al a ccc Pr o 250 at t SIle ttt Phe gct Al a ttg Leu Gy gt g Val gcg Al a cct Pr o gat Asp ggc Gy 140 gag G u gtt Val gtt Val cgg Ar g cat Hi s 220 ttt Phe ttc Phe ttg Leu ctt Leu ggc Gy 300 act Thr Gene Sequence 24-2-06.txt lie y Arg Ala Leu Tyr Pro n Asp t at Tyr gt g Val cct Pr o 110 ggc Gy aag Lys gag G u cgg Ar g gag G u 190 aat Asn gt g Val cat Hi s act Thr ttg Leu 270 cct Pr o cac Hi s ttg Leu gct Al a 80 gcg Al a ccg Pr o cag G n ttt Phe gag G u 160 acg Thr gt g Val gag G u ggc G y cat Hi s 240 gcg Al a cgc Ar g gcc Al a at g bet ccg Pr o 320 cgc Ar g ggt Gy ttg Leu at c SIle ttg Leu 145 gcg Al a gt g Val tcg Ser cgg Ar g cgc Ar g 225 G y ttc Phe gca Al a gat Asp ct c Leu 305 gag G u t at Tyr gct Al a at t SIle acg Thr 130 t ct Ser cat Hi s cgc Ar g ttg Leu ggc G y 210 gcg Al a gt g Val ccc Pr o aag Lys cgt Ar g 290 tct Ser t at Tyr tcg Ser ttt Phe 100 tgg Tr p tcg Ser ggt Gy aat Asn act Thr 180 act Thr ctt Leu gcc Al a gct Al a ccg Pr o 260 aag Lys gct Al a gat Asp ggt Gy cat Hi s gag G u ttg Leu cat Hi s cgc Ar g gct Al a 165 gct Al a cct Pr o cgc Ar g aat Asn cag G n 245 gaa G u aac Asn gt g Val gag G u gaa 325 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 ggc cca cgt ccc gtg gtt gcc acc acg gtg gtg tct tcg cag ctt ctg Page Gy Pro Arg Pro Val Val Ala 330 Gene Sequence 24-2-06.txt Thr Thr Val Val Ser Ser G n Leu Leu 335 340 ggt atc at c S y lie l ie gga ttc aaa G y Phe Lys 360 gct ttc gct Al a Phe Al a 375 gtg ccg gat Val Pro Asp 390 gct gcc gaa Al a Al a G u gag ttg tat G u Leu Tyr cgc acg agc Arg Thr Ser 440 cag caa gaa Gn n u 455 aaa cag ggc Lys G n a y 470 gct att ggt Al a l e G y gaa gtt ggt Su Val y cag ctg gag G n Leu u 520 gag G u ct g Leu gag G u gac Asp aag Lys 410 cga Ar g cca Pr o at t SIle gct Al a gt c Val 490 gcc Al a aaa Lys agg Ar g gct Al a 380 at c SIle cag G n ggg G y gag G u gt g Val 460 cat Hi s gga G y tcc Ser ggg a y gct Al a 365 gt g Val tct Ser ggc G y tat Tyr tta Leu 445 tct Ser ggc G y act Thr cat Hi s tgg Tr p 350 gcc Al a ggc G y aca Thr gca Al a ttt Phe 430 gtt Val gt c Val cag G n gag G u gat Asp 510 gat Asp gat Asp acc Thr gcg Al a agc Ser 415 gcg Al a gat Asp acc Thr gt g Val gcg Al a 495 gaa G u t at Tyr ggt G y tgc Cys ttg Leu 400 ct g Leu tcc Ser cac Hi s cca Pr o ggg G y 480 aaa Lys gca Al a t cc Ser ct c Leu ccg Pr o 385 ttc Phe cag G n tcg Ser tgg Tr p cat Hi s 465 cat Hi s gcc Al a gct Al a gag G u gac Asp 370 gtt Val at g bet caa G n caa G n att SIle 450 att SIle gt g Val aag Lys cag G n aca Thr 355 gga G y cca Pr o gcg Al a aaa Lys at t SIle 435 gcg Al a ctt Leu cat Hi s ct c Leu ttg Leu 515 ct g Leu ccg Pr o gat Asp tcg Ser ct c Leu 420 gct Al a cat Hi s cct Pr o at c SIe t at Tyr 500 ttg Leu 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1700 1713 gaa gt c caa Su Val n agc tgg ttg agc aag ctt Ser Trp Leu Ser Lys Leu 525 530 t agt t t cct g gctgctcccg gtt <210> 32 <211> 530 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 32 IVet Asp u Ser Arg G n Leu Ser Phe y Thr Ala y Leu Arg Ala 1 5 10 Pro Val G y Pro Ala Arg His G n et Asn Val Leu G n Val Thr Arg 25 Thr Thr Ala G y Val Al a Ser Trp Leu Ala G u Arg Ala Ala Leu Asn 40 Page 41 Gene Sequence 24-2-06.txt Pro Val Pro His Leu Val Pro G u Asp G u Thr Leu Ar g Gy Leu I le Leu 145 Al a Val Ser Ar g Ar g 225 Gy Phe Al a Asp Leu 305 G u Ser Ser Leu Pr o 385 Pr o Gy Gy Pr o 115 Al a Asn l Ie Pr o Val 195 Asn let Lys Asn G u 275 Cys Gy Ser G n Thr 355 Gy Pr o Asp Hi s G u Leu Hi s Ar g Al a 165 Al a Pr o Ar g Asn G n 245 G u Asn Val G u G u 325 Leu Thr Leu Val Gy 70 Thr Val Val Asn G n 150 Val Asp Asp Val Al a 230 G n G u Al a Gy Val 310 Gy Gy Gy Al a Val 390 Pr o Phe Thr Asn Gy 135 Leu G u G n G n Val 215 Phe Tyr Pr o Asp SIle 295 Gy Pr o SIle Phe Phe 375 Pr o Leu Al a Leu Lys 120 Al a Tyr Asp Leu Al a 200 Tyr G n Pr o Ser SIle 280 Ar g Thr Ar g SIle Lys 360 Al a Asp Ar g Al a Leu 105 Hi s Al a Ser Pr o Ar g 185 Asp Thr Phe Asp Al a 265 Leu Thr Leu Pr o Al a 345 Asn Tyr Lys Val Thr 90 Pr o Sy Asp G u SIle 170 Ar g Leu Al a Al a Pr o 250 l le Phe Al a Leu Val 330 G u Leu G u Asp Val Thr Thr Leu Asn Leu 155 Ar g Tyr Leu Leu Sy 235 Thr G u Al a Asp Al a 315 Val Asp Ser G u Sy 395 Sy Val Al a Pr o Asp Sy 140 G u Val Val Ar g Hi s 220 Phe Phe Leu Leu Sy 300 Thr Al a Lys Ar g Al a 380 I le lie y Arg Ala Sy G u Ser Al a 125 Tyr Pr o Pr o Asp Val 205 Sy Pr o Pr o Leu Asp 285 Sy Ar g Thr Sy Al a 365 Val Ser Tyr Val Pr o 110 Sy Lys G u Ar g G u 190 Asn Val Hi s Thr Leu 270 Pr o Hi s Leu Thr Tr p 350 Al a Sy Thr Asp Phe Thr Val Val Leu Val 175 Met Ser Sy Thr Val 255 G u Asp Ar g Val Val 335 Asp Asp Thr Al a Phe IVet Ala Ser Trp Al a Ala u Leu Lys Al a n y Ala Ser Leu Page 42 Gene Sequence 24 405 410 Sn G n Lys Leu Asn G u Leu Tyr Arg Arg Tyr 420 425 Ser G n Ile Ala Val Arg Thr Ser Ser Pro Arg 435 440 Trp lie Ala His Pro G n n G u Leu IIe G y 450 455 His lie Leu Pro G u Lys n G y lie Al a Leu 465 470 475 His Val His Ile Arg Al a I Ie G y Arg Val Ser 485 490 Ala Lys Leu Tyr Leu G u Val y G n Ala Ser 500 505 Ala G n Leu Leu His G n Leu G u Asp G u Val 515 520 Lys Leu 530 <210> 33 <211> 1684 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1684) <223> FRXA02854 <400> 33 aatacctttc tgttttgtcc gcaggcgtat caggaaaacc tct aatagtg atcccacgaa aacaaaggat cggggtgttc -2-06. txt G y Tyr Phe 430 G u Leu Val 445 Val Ser Val 460 Hi s G y G n G y Thr u Ser His Asp 510 G n Ser Trp 525 415 Al a Asp Thr Val Al a 495 G u Leu cag G n cgc Ar g gct Al a gtt Val ggt Sy act Thr agt Ser cag G n tgg Tr p gag G u ttg Leu gct Al a ggc Sy aat Asn gca Al a gaa G u gt c Val acc Thr aca Thr gtt Val gaa G u aca Thr gtt Val 75 act Thr gca Al a ttg Leu cgt Ar g gga G y gt g Val gcg Al a ttg Leu gt a Val gca Al a ggc G y t at Tyr gt g Val cgt gca Arg Ala 15 acc aga Thr Arg ct a aat Leu Asn agg gcg Arg Ala gac gct Asp Al a 80 ttc gcg Phe Al a 95 Page 43 tgcagcgcgg tagagtcgag atg gac gag tct cgt IVet Asp G u Ser Arg 1 cca gtt ggc ccg gcg Pro Val y Pro Ala act aca gca ggt gtt Thr Thr Al a y Val cca gtg ccg cat ttg Pro Val Pro His Leu ttg tat ccc caa gat Leu Tyr Pro G n Asp cgc tat ggt tcg cat Arg Tyr G y Ser His ggt gct ggt ttt gag G y Al a y Phe u 100 Gene Sequence 24-2-06.txt gtg acg ttg ctc ccc acg cct agc Val Thr Leu Leu Pro Thr Pro Ser cct acg ccg ttg att Pro Thr Pro Leu Ile ccg tgg ttg Pro Tr p Leu 115 gt g Val aat Asn cag G n 150 gt g Val gat Asp gat Asp gt g Val gct Al a 230 cag G n gag G u gct Al a ggt Gy gt g Val 310 ggc Gy ggt Gy aag Lys 120 gcg Al a t at Tyr gat Asp ct g Leu gct Al a 200 t at Tyr caa G n cct Pr o tct Ser at t l Ie 280 cgt Ar g aca Thr cgt Ar g at c SIle ggg Gy gac Asp gaa G u at t SIle 170 cga Ar g ttg Leu gct Al a gct Al a ccc Pr o 250 at t SIle ttt Phe gct Al a ttg Leu gt g Val 330 gag G u gat Asp ggc Gy 140 gag G u gtt Val gtt Val cgg Ar g cat Hi s 220 ttt Phe ttc Phe ttg Leu ctt Leu ggc Gy 300 act Thr gcc Al a aaa Lys gcg Al a 125 t ac Tyr cct Pr o cct Pr o gat Asp gt g Val 205 ggt Gy ccc Pr o ccc Pr o ttg Leu gat Asp 285 ggc Gy cgt Ar g acc Thr ggg Gy 110 ggc Gy aag Lys gag G u cgg Ar g gag G u 190 aat Asn gt g Val cat Hi s act Thr ttg Leu 270 cct Pr o cac Hi s ttg Leu acg Thr tgg Tr p 350 cag G n ttt Phe gag G u 160 acg Thr gt g Val gag Gu ggc Gy cat Hi s 240 gcg Al a cgc Ar g gcc Al a at g Net ccg Pr o 320 gt g Val t at Tyr ggt Gy at c SIle ttg Leu 145 gcg Al a gt g Val tcg Ser cgg Ar g cgc Ar g 225 ggc Gy ttc Phe gca Al a gat Asp ct c Leu 305 gag G u tct Ser t cc Ser ct c Leu acg Thr 130 tct Ser cat Hi s cgc Ar g ttg Leu ggc Gy 210 gcg Al a gt g Val ccc Pr o aag Lys cgt Ar g 290 tct Ser t at Tyr tcg Ser gag G u gac Asp 370 gct Al a aat Asn at c SIle ccc Pr o gt g Val 195 aat Asn at g bet aag Lys aat Asn gaa G u 275 tgt Cys ggc Gy t cc Ser cag G n aca Thr 355 gga Gy tcg Ser ggt Gy aat Asn act Thr 180 act Thr ctt Leu gcc Al a gct Al a ccg Pr o 260 aag Lys gct Al a gat Asp ggt Gy ctt Leu 340 ct g Leu ccg Pr o cat Hi s cgc Ar g gct Al a 165 gct Al a cct Pr o cgc Ar g aat Asn cag G n 245 gaa G u aac Asn gt g Val gag G u gaa G u 325 ct g Leu acg Thr ctt Leu 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 gga ttc aaa G y Phe Lys 360 aat ctg tcg agg gct gcc gat Asn Leu Ser Arg Ala Ala Asp 365 Page 44 Gene Sequence 24-2-06.txt gct ttc gct tat gag gaa gct gtg ggc Ala Phe Ala Tyr u u Ala Val y acc tgc ccg Thr Cys Pro gt g Val 390 gct Al a gag G u cgc Ar g cag G n aaa Lys 470 gct Al a gaa G u cag G n gat Asp gaa G u t at Tyr agc Ser 440 gaa G u ggc Gy ggt Sy ggt Sy gag G u 520 gac Asp aag Lys 410 cga Ar g cca Pr o at t SIle gct Al a gt c Val 490 gcc Al a gaa G u 380 at c SIle cag G n ggg G y gag G u gt g Val 460 cat Hi s gga Sy t cc Ser caa G n aca Thr gca Al a ttt Phe 430 gtt Val gt c Val cag G n gag G u gat Asp 510 tgg Tr p gcg Al a agc Ser 415 gcg Al a gat Asp acc Thr gt g Val gcg Al a 495 gaa G u ttg Leu ttg Leu 400 ct g Leu t cc Ser cac Hi s cca Pr o ggg Gy 480 aaa Lys gca Al a agc Ser 385 ttc Phe cag G n tcg Ser tgg Tr p cat Hi s 465 cat Hi s gcc Al a gct Al a gtt Val at g NMbt caa G n caa G n at t SIle 450 at t SIle gt g Val aag Lys cag G n cca gat gt c Pro Asp Val gcg Al a aaa Lys at t SIle 435 gcg Al a ctt Leu cat Hi s ct c Leu ttg Leu 515 tcg Ser ct c Leu 420 gct Al a cat Hi s cct Pr o at c SIe t at Tyr 500 ttg Leu 1267 1315 1363 1411 1459 1507 1555 1603 1651 1684 <210> 34 <211> 528 <212> PRT <213> Corynebacter i um gl ut am cum <400> 34 lIet Asp u Ser Arg G n Leu Ser Phe 1 5 Pro Val G y Pro Ala Arg His G n lVet 25 Thr Thr Ala y Val Al a Ser Trp Leu 40 Pro Val Pro His Leu Val Pro G u Asp 55 Leu Tyr Pro G n Asp G y Pro Leu Arg 70 Arg Tyr G y Ser His Thr Phe Ala Ala Sy Ala y Phe G u Val Thr Leu Leu 100 105 G y Thr Al a 10 Asn Val Leu Al a G u Ar g G u Thr G y Val Val Val 75 Thr Thr Al a 90 Pro Thr Pro Page Sy G n Al a I le Sy G u Ser Leu Val Al a Sy Tyr Val Pr o 110 Ar g Thr Leu Ar g Asp Phe Thr Al a Ar g Asn Al a Al a Al a Pr o Gen e Leu Ilie Pro Trp Leu Val Asn 115 Sequence 24-2- 06. txt Hi s 0y Leu Asp Al aC I Ie Le u 145 A a ValI Ser Ar g Ar g 225 O31y Ph e A a As p Le u 305 O31u Ser Ser Le u Pr o 385 Ph e O31n Ser Tr p A a As n I Ie Pr o ValI 1 95 As n vb t Ly s As n O31u 275 cys O31y Ser O31n Thr 355 O31y Pr o A a Ly s I Ie 435 A a Hi s Ar g A a 1 65 A a Pr a Ar g As n O31n 245 O31u As n ValI O31u O31u 325 Le u Thr Le u ValI Tr p 405 As n ValI Pr o As n O31n 150 ValI As p As p ValI A a 230 O31n O31u A a O31y ValI 310 O31y O31y O31y A a ValI 390 A a O31u Ar g O31n Ly s 470 O31y 135 Le u O31u O31n O31n ValI 215 Ph e Tyr Pr a As p I Ie 295 O31y Pr a I Ie Ph e Ph e 375 Pr a A a Le u Thr O31n 455 Ly s 120 A a Tyr As p Le u A a 200 Tyr O31n Pr a Ser I Ie 280 Ar g Thr Ar g I Ie Ly s 360 A a As p O31u Tyr Ser 440 O31u 125 3 y Val O31n A a Ser Pr a Ar g 1 As p Thr Ph e As p A a 265 Le u Thr Le u Pr a A a 345 As n Tyr Ly s Le u Ar g 425 Ser Le u As p O31u I Ie 1 70 Ar g Le u A a A a Pr a 250 li1e Ph e A a Le u ValI 330 O31u Le u O31u As p Ly s 410 Ar g Pr a I Ie As n Le u 1 55 Ar g Tyr Le u Le u O31y 235 Thr O31u A a As p A a 315 ValI As p Ser O31u O31y 395 A a Tyr Ar g O31y Tyr Pr a Pr a As p ValI 205 O31y Pr a Pr a Le u As p 285 O31y Ar g Thr O31y A a 365 ValI Ser O31y Tyr Le u 445 Ser ValI Le u ValI 1 vb t Ser O31y Thr ValI 255 O31u As p Ar g ValI ValI 335 As p As p Thr A a Ser 415 A a As p Thr Ph e O31u 1 Thr ValI O31u O31y Hi s 240 A a Ar g A a vb t Pr a 320 ValI Tyr O31y cys Le u 400 Le u Ser Hi s Pr a (31y 480 His Ilie Leu Pro Gu 465 O n y I e Al a Leu His 5 y n Val 475 Page 46 Gene Sequence 24- 2- 06. txt His Val His IIe Arg Ala IIe y Arg Val Ser G y Thr G u Ala Lys 485 490 495 Ala Lys Leu Tyr Leu G u Val G y G n Ala Ser Ser His Asp G u Ala 500 505 510 Ala G n Leu Leu His G n Leu G u Asp G u Val G n Ser Trp Leu Ser 515 520 525 <210> <211> 536 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> <223> RXA00511 <400> gag ctg cgc aag aac acc ctc gtg ggc act gtc atg agc aac ctg gga 48 G u Leu Arg Lys Asn Thr Leu Val y Thr Val IVet Ser Asn Leu y 1 5 10 ttg aag att gct atg gat gaa gcc gga att aca ctg cgt acc acc aag 96 Leu Lys lie Ala IVet Asp G u Ala Gy lie Thr Leu Arg Thr Thr Lys 25 gta gga gac cgc tac gtg ctg gaa gac ctc aat gca ggt gga ttc agc 144 Val y Asp Arg Tyr Val Leu G u Asp Leu Asn Ala Gy y Phe Ser 40 ctg ggc ggc gag caa tct ggc cac att gtt ctt cca gat cat ggc acc 192 Leu G y G y G u n Ser G y His IIe Val Leu Pro Asp His G y Thr 55 act ggc gat gga act ttg act ggt ctt tcc atc atg gcg cgc atg gct 240 Thr y Asp y Thr Leu Thr y Leu Ser Ile IVet Ala Arg IVet Ala 70 75 gaa acc gga aag tcc ttg ggc gag ttg gca caa gct atg acg gtg ctg 288 G u Thr G y Lys Ser Leu G y G u Leu Ala G n Ala IVet Thr Val Leu 90 cca cag gtt ctg atc aat gtg cca gtt tcg gat aag tcc acc atc gtg 336 Pro G n Val Leu Ile Asn Val Pro Val Ser Asp Lys Ser Thr lie Val 100 105 110 agc cac cca agc gtt gtg gct gcg atc gcg gaa gca gaa gct gag ttg 384 Ser His Pro Ser Val Val Ala Ala lle Ala G u Ala G u Ala G u Leu 115 120 125 ggc gcc acc ggt cgc gtt ctt ctt cgt gct tct ggc acc gaa gag ctt 432 Sy Ala Thr Gy Arg Val Leu Leu Arg Ala Ser Gy Thr G u G u Leu 130 135 140 ttc cgc gtg atg gtt gag gct gga gac aag gaa caa gct cgt cgt atc 480 Phe Arg Val IVet Val u Al a G y Asp Lys G u G n Al a Arg Arg lie 145 150 155 160 gcg gga cgt ctt gct gca gtg gtt gca gaa gtc taattcactt cagtcacagc 533 Al a y Arg Leu Ala Ala Val Val Ala G u Val 165 170 Page 47 ene Sequence 24- 2- 06. txt gca 536 <210O> 36 <211> 171 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 36 O31u Leu Arg Lys Asn Thr Leu Val G31y Thr Val I'bt Ser Asn Leu G31y Le u ValI Le u Thr G u Pr o Ser 0Gy Ph e 145 Al a I Ie As p G31y As p G y ValI Pr o 115 Thr ValI Ar g vb t Tyr O31n Thr Ser li1e ValI Ar g ValI G31u Le u G31y Thr G y ValI Al a Le u 135 Al a Al a G31u 40 Hi s G31y G u Pr o Al a 120 Le u (31y G31y As p I Ie Le u Le u ValI 1 05 li1e Ar g As p 10 I Ie Le u ValI Ser Al a 90 Ser Al a Al a Ly s Thr As n Le u I Ie G n As p 0Gu Ser 0Gu 1 Le u Al a Pr a vb t Al a Ly s Al a G31y 140 O31n Ar g G31y As p Al a vb t Ser O31u 125 Th r Al a Thr G31y Hi s Ar g Thr Thr 110 Al a O31u Ar g Thr Ph e G31y vb t ValI lie O31u O31u Ar g Leu Ala Ala Val Val Ala Ou Val <210O> 37 <21 1> 1497 <212> DNA <213> Cor ynebact er i urn gi ut ani curn <220> <221 CDS <222> (1474) <223> RXN01365 <400> 37 cct gat cagg acgaat cat a aggt t tgct a t tcggat tgg at cct t tggc aggggt agga t tgcaagcgt t att t tgt tc cct aacccct t cgaggat tt at g cgt acc cgt gaa 115 I'b t Ar g Thr Ar g O31u 1 t ct gt c aca gct gt a at t aag gcg t at gac gt c cgt ggt gt t gt t ggt 163 Ser Val Thr Al a Val Ilie Lys Ala Tyr Asp Val Ar g G31y Val Val G31y 15 gt c gat at t gat gct gat ttc att tct gag act ggc gct gcc t tt ggt 211 Val Asp I Ie Asp Al a Asp Phe I Ie Ser C3u Thr C3y Al a Al a Phe C3y 30 Page 48 Gene Sequence 24-2-06.txt cgg Ar g at g let act Thr gag G u act Thr gcg Al a gat Asp gtt Val 150 ctt Leu gca Al a cca Pr o aac Asn aag Lys 230 gat Asp cct Pr o ccg Pr o gag G u at g let gat Asp cag G n t ac Tyr tcg Ser gct Al a 120 ct g Leu gag Gu gat Asp ggc Gy gat Asp 200 gag Gu acc Thr gat Asp gcg Al a t cc Ser 280 at t SIle agt Ser t cc Ser ttg Leu gcg Al a aac Asn ccg Pr o gag Gu gat Asp aag Lys 170 ggt Gy gcg Al a aat Asn gag G u tgc Cys 250 tgt Cys at c SIle gaa Gu gag Gu cct Pr o gat Asp 75 t cc Ser ccc Pr o gt c Val ggt Gy tt g Leu 155 aac Asn ggg Gy cca Pr o cct Pr o acc Thr 235 tt c Phe gcg Al a at c SIle aac Asn ggt gaa Gy Gu 45 gaa ttg G u Leu gtt gtt Val Val gga acc G y Thr gct gag Al a G u ggt cag Gy n 125 gtt cca Val Pr o 140 ctg agc Leu Ser atc cgc li e Ar g ttc act Phe Thr ct g t at Leu Tyr 205 ctg gag Leu G u 220 gga tct G y Ser gtg gt c Val Val at c gt a I e Val cac aac His Asn 285 ggt ggc Gy Gy 300 acc acc gtt gct Thr Thr Val Al a gcc Al a cat Hi s ttg Leu t ac Tyr 110 gat Asp gcg Al a gca Al a ccg Pr o gt c Val 190 ttt Phe cct Pr o gat Asp gat Asp gcg Al a 270 ct g Leu act Thr gcg Al a gga G y tgt Cys ggc G y ggt G y gat Asp gcc Al a 160 aag Lys gag G u ctt Leu aac Asn ggt G y 240 aag Lys cgt Ar g acc Thr ttt Phe ct g Leu gct Al a at c SIle ttg Leu ggt Gy 145 gag G u gtt Val gt a Val gac Asp ct g Leu 225 ttg Leu ggc G y t ac Tyr tct Ser at t I e gcc Al a act Thr ggt G y aag Lys gcc Al a 130 gag G u t ac Tyr gct Al a ttc Phe ggc Gy 210 gtt Val gcg Al a cag G n ttg Leu aag Lys 290 ggc cat gac G y Hi s Asp gat Asp tct Ser gcg Al a ttg Leu 115 aac Asn t ca Ser ct c Leu gt g Val aag Lys 195 aat Asn gat Asp ttc Phe cca Pr o gag G u 275 gct Al a ggc G y act Thr at g let 100 tgt Cys at c SIe ggt Gy aat Asn gat Asp 180 ggt Gy ttc Phe ttg Leu gac Asp gt c Val 260 aag Lys gt g Val 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 gcg gtg cgt Al a Val Ar g 305 Page 49 act cgc gtg ggt Thr Arg Val G y Gene Sequence 24-2-06.txt cac tcc ttc atc aag gcg His Ser Phe Ile Lys Ala 310 315 ggc gag cac tct gcg cac G y G u His Ser Ala Hi s 330 tcc ggc att ttg gct gcg Ser G y Ile Leu Ala Al a 345 gac cag cca ctc agt gag Asp G n Pro Leu Ser G u 360 tca ggc gag ttg aac tcc Ser G y G u Leu Asn Ser 375 cgc acc cag gct gtg ctc Arg Thr G n Ala Val Leu 390 395 gac acc ctt gac ggc gtg Asp Thr Leu Asp G y Val 410 ttc aac gtg cgt gcg tcc Phe Asn Val Arg Ala Ser 425 gaa gct gca tcg aag gaa G u Ala Ala Ser Lys G u 440 ggg att atc cgc gca taat G y Ile Ile Arg Al a 455 <210> 38 <211> 458 <212> PRT <213> Corynebacter i um gl <400> 38 IVet Arg Thr Arg u Ser 1 5 Arg y Val Val y Val G y Ala Ala Phe G y Ar g Al a lI e G y Hi s Asp IVet Phe Ala Asp y Val Thr 70 Leu Thr Ser Thr Asp G u Ala G y Ala Iet Phe Thr 100 aag Lys t ac Tyr at g et at g et cgt Ar g 380 gat Asp act Thr aac Asn gaa G u atg gca gag [et Ala G u tac ttc act Tyr Phe Thr 335 cac gtg ct g Hi s Val Leu 350 atg gct agg IVet Al a Arg 365 ttg gct aat Leu Al a Asn gcg ttc gct Al a Phe Al a gt g gaa ct c Val G u Leu 415 acc gag ccg Thr G u Pro 430 gtc gat gcg Val Asp Al a acc ggt gcg gcc ttt Thr G y Al a Al a Phe 320 gag ttc G u Phe gct gcg Al a Al a tat aac Tyr Asn gca gag Al a u 385 gat cgc Asp Arg 400 aag gac Lys Asp ctg ctt Leu Leu ttg gta Leu Val ttc Phe ct g Leu cgg Ar g 370 gcg Al a acc Thr acc Thr cgc Ar g gcg Al a 450 aat Asn gga G y 355 t ac Tyr cag G n gag G u t cc Ser ct c Leu 435 gag G u gcg Al a 340 agc Ser gtt Val caa G n t cc Ser gcg Al a 420 aat Asn at t SIle ggt G y 325 gac Asp cag G n gct Al a gag G u gt g Val 405 tgg Tr p gtt Val ct a Leu 1075 1123 1171 1219 1267 1315 1363 1411 1459 1497 cccatt ttccggcggg cat ut ari cum Val Thr Al a Asp I e Asp 25 Leu IVet Ar g 40 Arg Asp Ser Al a G n y Leu Tyr Phe Al a Ser Hi s 105 Val lile 10 Al a Asp Ser G u Ser Pro Leu Asp 75 Al a Ser 90 Asn Pro Page Al a SIle G u Leu Val Thr G u Tyr Ser Thr Al a Hi s Leu Tyr 110 Asp G u Thr Lys Leu Lys Asn Val Thr Val Al a Sy Cys Gy ene Sequence 24- 2- 06. txt I Ie Le u G31y 145 O31u ValI ValI As p Le u 225 Le u G31y Ty r Ser Ar g 305 G31y Ph e A a As n O31u 385 Ar g As p Le u ValI Le u 115 As n Ser Le u ValI Ly s 1 95 As n As p Ph e Pr o O31u 275 A a Ar g A a As n G31y 355 Ty r O31n O31u Ser Le u 435 O31u Cy s Ar g Ala G31y Ala Ar g Pro ValI 03 y 03 n As p Se r 03 y 125 I Ie Ser O31u 1 65 A a Le u Pr o O31n (31y 245 Ser Le u Pr o G31y G31y 325 As p O31n A a (31u ValI 405 Tr p ValI Le u As p ValI 150 Le u A a Pr a As n Ly s 230 As p Pr a Pr a (31u Hi s 310 G31y Ser As p Ser Ar g 390 As p Ph e (31u G31y As p 135 Ser ValI As n Le u Hi s 215 Ph e A a Ser G31y ValI 295 Ser (31u G31y O31n O31y 375 Thr Thr As n A a I Ie 455 120 Le u O31u As p O31y As p 200 O31u Thr As p A a Ser 280 li1e Ph e Hi s I Ie Pr a 360 O31u O31n Le u ValI A a 440 li1e ValI O31n Le u vb t 1 85 ValI A a ValI Ar g I Ie 265 Thr A a I Ie Ser Le u 345 Le u Le u A a As p Ar g 425 Ser Ar g O31u As p Ly s 1 70 O31y A a As n O31u Cys 250 Cys I Ie O31u Ly s A a 330 A a Ser As n ValI O31y 410 A a Ly s A a O31y Le u 1 As n O31y Pr a Pr a Thr 235 Ph e A a I Ie As n A a 315 Hi s A a O31u Ser Le u 395 ValI Ser (31u Pr a Ser Ar g Thr Tyr 205 O31u Ser ValI ValI As n 285 O31y Tyr Hi s vb t 365 Le u A a ValI Thr ValI 445 A a A a Pr a ValI 1 Ph e Pr a As p As p A a 270 Le u Thr A a Ph e ValI 350 A a A a Ph e O31u O31u 430 As p Ph e Tyr vb t 1 Pr a O31u A a I Ie (31u 255 Ou le A a O31u Thr 335 Le u Ar g As n A a Le u 415 Pr a A a Page 51 Gene Sequence 24-2-06.txt <210> 39 <211> 994 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(994) <223> FRXA01365 <400> 39 cctgatcagg acgaatcata aggtttgcta ttcggattgg atcctttggc aggggtagga ttgcaagcgt tattttgttc cctaacccct tcgaggattt tct Ser gt c Val cgg Ar g at g Net act Thr gag G u act Thr gcg Al a gat Asp gtt Val 150 ctt Leu gca Al a aca Thr at t SIle at g let gat Asp cag G n t ac Tyr tcg Ser gct Al a 120 ct g Leu gag G u gat Asp ggc G y gt a Val gct Al a agt Ser t cc Ser ttg Leu gcg Al a aac Asn ccg Pr o gag Su gat Asp aag Lys 170 ggt Sy aag Lys ttc Phe ggt Gy gaa Gu gtt Val gga Gy gct Al a ggt G y gtt Val 140 ct g Leu at c SIle ttc Phe gcg Al a at t SIle gaa Gu 45 ttg Leu gtt Val acc Thr gag G u cag G n 125 cca Pr o agc Ser cgc Ar g act Thr t at Tyr 205 tat gac Tyr Asp 15 tct gag Ser G u acc acc Thr Thr gcc aag Al a Lys cat ttg Hi s Leu ttg aag Leu Lys 95 tac aac Tyr Asn 110 gat tct Asp Ser gcg ttt Al a Phe gca t at Al a Tyr ccg atg Pro lbet 175 gt c cct Val Pr o 190 ttt gag Phe G u gt c Val act Thr gtt Val gcg Al a gga Gy 80 tgt Cys ggc Gy ggt G y gat Asp gcc Al a 160 aag Lys gag G u ctt Leu at g bet 1 cgt Ar g ggc G y gct Al a ttt Phe ct g Leu gct Al a at c SIle ttg Leu ggt G y 145 gag G u gtt Val gt a Val gac Asp ggt G y gct Al a at t I e gcc Al a act Thr ggt Gy aag Lys gcc Al a 130 gag G u t ac Tyr gct Al a ttc Phe ggc Gy 210 gtt Val ttt Phe cat Hi s ggc Gy act Thr at g let 100 tgt Cys at c l Ie ggt G y aat Asn gat Asp 180 ggt G y ttc Phe cgt acc cgt Arg Thr Arg gaa Gu ggt G y ggt G y gac Asp gtg Val gat Asp ttt Phe cgt Ar g att SIe tcg Ser gag G u 165 gcg Al a ctg Leu ccc Pr o cca ctt gat Pro Leu Asp 200 gtt gcg cca ctg Val Ala Pro Leu Page 52 aac cat gag Asn His G u 215 aag ttt acc Lys Phe Thr 230 gat gcg gat Asp Al a Asp cct tcg gcg Pro Ser Ala ccg ggt t cc Pro G y Ser 280 gag gt g at t Gu Val lle 295 <210> <211> 298 <212> PRT aat Asn gag G u tgc Cys 250 tgt Cys at c SIle gaa Gu cct Pr o acc Thr 235 tt c Phe gcg Al a at c SIle ct g Leu 220 gga G y gt g Val at c l Ie cac Hi s Gene Sequence 24-2-06.txt gag cct gcc aac ctg gtt gat G u Pro Ala Asn Leu Val Asp 225 t ct gat at c ggt ttg gcg ttc Ser Asp Ile G y Leu Ala Phe 240 gtc gat gag aag ggc cag cca Val Asp u Lys Gy n Pro 255 gta gcg gag cgt tac ttg gag Val Ala G u Arg Tyr Leu G u 270 275 aac ctg att acc tct aag gct Asn Leu Ile Thr Ser Lys Ala 285 290 ttg Leu gac Asp gtc Val 260 aag Lys gtg Val <213> Corynebacter i um gl ut ai cum <400> IVet Arg 1 Arg G y G y Al a Al a ll e Phe Al a Leu Thr Al a G y I e Lys Leu Al a 130 G y G u 145 G u Tyr Thr Arg u Ser Val Al a G y Asp Ser Al a Leu 115 Asn Ser Leu 5 Gy Gy Asp Val Asp Phe Ar g SIle Ser G u 165 Val Asp Leu Ar g Al a Leu Al a Gy Asp 135 Ser Val Al a Asp Ar g Ser Gy Phe Hi s 105 Ar g Val G n Leu Val 10 Al a Ser Ser Leu Al a 90 Asn Pr o G u Asp Lys 170 SIle Asp G u Pr o Asp 75 Ser Pr o Val Gy Leu 155 Asn Al a SIle G u Leu Val Thr G u G n 125 Pr o Ser Ar g Tyr Ser Thr Al a Hi s Leu Tyr 110 Asp Al a Al a Pr o Asp G u Thr Lys Leu Lys Asn Ser Phe Tyr 1et 175 Val Thr Val Al a Gy Cys Gy Gy Asp Al a 160 Lys Val Al a Val Ala Ala Asn Gy Iet Gy Gy Phe Thr Val Pro u Page 53 Gene Sequence 24 Val Phe Lys y Leu Pro Leu Asp Val Ala Pro 195 200 Asp G y Asn Phe Pro Asn His G u Ala Asn Pro 210 215 Leu Val Asp Leu G n Lys Phe Thr Val G u Thr 225 230 235 Leu Al a Phe Asp G y Asp Al a Asp Arg Cys Phe 245 250 Gy G n Pro Val Ser Pro Ser Ala Ile Cys Al a 260 265 Tyr Leu G u Lys Leu Pro y Ser Thr lie lie 275 280 Ser Lys Ala Val Pro G u Val I e Ala G u 290 295 <210> 41 <211> 1743 <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(1720) <223> RXA00098 <400> 41 taaatttgtc gtgtttccca ctttgaacac t cttcgatgc taacctgaag atgttattta acgacaataa aggagttttc -2-06. txt Leu Tyr Phe 205 Leu G u Pro 220 G y Ser Asp Val Val Asp I e Val Al a 270 His Asn Leu 285 G u Al a SIe G u 255 G u lie gcttggccac aaaagcaagc acc Thr gca Al a t ac Tyr ctt Leu ggc Gy aac Asn gaa G u cag G n act Thr ttc Phe gac Asp cgc Ar g gaa G u gat Asp tgg Tr p cgt Ar g gcg Al a acc Thr cgc Ar g cgc Ar g t ca Ser caa G n gaa G u gct Al a ct c Leu at t SIle 75 gct Al a gt a Val gac Asp ctt Leu ggc G y acc Thr gac Asp gt c Val gat Asp acc Thr aag Lys cac Hi s ct c Leu at g Net cac Hi s caa G n 110 gat Asp 15 gaa G u gt c Val ctt Leu ttt Phe acc Thr 95 gat Asp cat Hi s gaa G u gac Asp gca Al a gcc Al a gcg Al a gtt Val at g et 1 t ac Tyr aac Asn ct g Leu ct g Leu ggt G y ct g Leu gct Al a gcg gac att Al a Asp I e t ca Ser cgc Ar g tcg Ser acc Thr gaa G u cgc Ar g gct Al a aac Asn gcc Al a aag Lys gaa G u cac Hi s ctt Leu gat Asp 115 ttc Phe gag G u aat Asn gaa G u ct c Leu cct Pr o 100 gtc Val gaa gtt ttg gga cgc atg cgt gac ttc gct act Page 54 gcg ctg cgc tca ggc G u Val Leu G y Ar g 120 Gene Sequence 24-2-06.txt IVet Arg Asp Phe Ala Thr Ala Leu Arg Ser y 125 130 aac Asn ggt Gy 150 cgt Ar g gac Asp aca Thr t cc Ser gag G u 230 gt c Val tgg Tr p at g let cac Hi s cca Pr o 310 gca Al a gct Al a cac Hi s ggt Gy ttg Leu ggt Gy t ac Tyr gca Al a ttc Phe 200 gct Al a gt c Val gag G u gga G y gt g Val 280 at g tet ttg Leu acc Thr t ac Tyr gac Asp 360 cct Pr o cac Hi s tct Ser acc Thr 170 ct c Leu at c SIle gca Al a aag Lys ggt Sy 250 cgt Ar g ggc G y gaa G u gct Al a gct Al a 330 cag G n t cc Ser aca Thr acc Thr gac Asp 155 gct Al a gtt Val gct Al a gct Al a cac Hi s 235 at c SIle tac Ty r cct Pr o cac Hi s ct g Leu 315 gt c Val cag G n cct Pr o aat Asn ggc G y 140 ct c Leu ggt Gy tct Ser tcg Ser cgt Ar g 220 ttc Phe gac Asp t cc Ser cgc Ar g ttc Phe 300 ct c Leu ct a Leu ct g Leu gtt Val ggc G3y 380 cac Hi s gga Gy at c SIle gt g Val aaa Lys 205 gct Al a gt c Val acg Thr gt g Val gac Asp 285 cgc Ar g ggt Gy cct Pr o acc Thr t cc Ser 365 cag G n acg Thr cca Pr o t ca Ser ttg Leu 190 act Thr tgg Tr p gca Al a gac Asp gac Asp 270 ttc Phe acc Thr gt c Val t at Tyr at g Net 350 act Thr cac Hi s at c SIle gcc Al a gca Al a 175 gaa G u ttc Phe ct g Leu gt g Val aac Asn 255 t cc Ser at g et acc Thr tgg Tr p t cc Ser 335 gaa G u ggc Gy gct Al a aag Lys at g Net 160 gaa G u gac Asp acc Thr gt a Val t cc Ser 240 at g Net gca Al a cgt Ar g aag Lys t ac Tyr 320 gag G u t ca Ser act Thr ttc Phe aag Lys 145 gct Al a ttc Phe ct c Leu acc Thr gag G u 225 acc Thr ttc Phe gtt Val ttc Phe ttc Phe 305 t cc Ser gat Asp aat Asn ggc Gy ttc Phe 385 at c SIle acg Thr gt c Val gat Asp cag G n 210 aag Lys aat Asn ggc G y ggt G y ct c Leu 290 gaa G u gat Asp ct c Leu ggc Gy gaa G u 370 cag G n aac Asn gct Al a aac Asn 180 gaa G u acg Thr ggt Gy gaa G u tgg Tr p 260 t cc Ser gga G y aac Asn t at Tyr cgt Ar g 340 t ca Ser t ac Tyr at c SIe 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 cag ggc act cgc ctt gtt cca gct gat ttc att ggt ttc gct cgt cca Page Sn G y Thr Arg Leu Val Pro 390 395 Gene Sequence 24-2-06.txt Ala Asp Phe Ile Gy Phe Ala Arg Pro 400 405 aag cag gat ctt cct gcc ggt gag cgc Lys G n Asp Leu Pro Al a G y G u Ar g 410 agc aac ttc ttc gca cag acc aag gtt Ser Asn Phe Phe Ala G n Thr Lys Val 425 430 gaa gag atc gct gcg gaa ggt gtc gca u u u Ile Ala Al a u y Val Al a 440 445 gtc atg cca ggt aat cgc cca acc acc Val IVet Pro y Asn Arg Pro Thr Thr 455 460 acc cct tct att ctc ggt gcg ttg atc Thr Pro Ser Ile Leu y Ala Leu Ile 470 475 atg gtt cag ggc gtg att tgg gac atc IVet Val n y Val Ile Trp Asp Ile 490 gtt gaa ctg ggc aaa cag cag gca aat Val u Leu Gy Lys Gn On Ala Asn 505 510 ggt gaa gag gat gtt gac tcg gga gat Sy u u Asp Val Asp Ser y Asp 520 525 aag tgg tac cgc gca aat agg tagtcgcl Lys Trp Tyr Arg Ala Asn Arg 535 540 <210> 42 <211> 540 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 42 IVet Ala Asp lie Ser Thr Thr G n Val 1 5 Tyr Ser Asn Phe G n Al a Thr Thr Leu Asn Arg Ala G u Lys Tyr Thr Phe Ser 40 Leu Ser Lys Asn Leu Leu Asp Asp Ala Leu Thr u u G uSer G y Leu Arg G u 70 G y G u Hi s Leu Asn Asn Thr G u Asp Leu Arg Leu Pro Ala G u Ala Asp Leu 100 105 Ala Ala Asp Val His G u Val Leu y at g et gct Al a gag G u at t SIle ttg Leu 480 t cc Ser ct c Leu t cc Ser cat Hi s ttc Phe ct g Leu ttg Leu 465 t ac Tyr ttc Phe gct Al a act Thr gac Asp ggt G y gt c Val 450 gcg Al a gaa G u gac Asp ccg Pr o gat Asp 530 ctt Leu aag Lys 435 aac Asn gag G u cac Hi s caa G n gct Al a 515 t ca Ser ttg Leu 420 aac Asn cac Hi s gaa G u at c SIle tgg Tr p 500 gt c Val ct g Leu at g et gct Al a aag Lys ctt Leu gt g Val 485 ggt G y tct Ser at t SIe 1363 1411 1459 1507 1555 1603 1651 1699 1743 :tg cttatagggt cag Trp n 10 Arg G u Al a Al a Thr Leu Arg Ile 75 Arg Ala 90 Ser Val Arg IVet Page 56 Leu Phe Leu Lys Al a Leu Sy Asp Asp G u Val Leu Phe Thr Asp Al a Hi s G u Asp Al a Al a Al a Val Thr Gene Sequence 24-2-06.txt 120 Leu G y Hi s Thr 125 His Thr Ile Lys Al a Lys 145 Al a Phe Leu Thr G u 225 Thr Phe Val Phe Phe 305 Ser Asp Asn Gy Phe 385 Gy Hi s Phe Leu Ar g Val Lys Ser Al a 195 G u Leu Al a Phe Leu 275 Gy G u Phe Ser Lys 355 I le Leu Al a Leu Lys 435 Asn Gy I e Leu 165 Val Ser Leu G u Lys 245 Asp Leu Phe Val Gy 325 Phe Val Tr p Hi s Pr o 405 bet Al a Lys Asn Gy 150 Ar g Asp Thr Ser G u 230 Val Tr p bet Hi s Pr o 310 Al a Al a Hi s Gy G n 390 Lys Ser G u Val Tr p 135 I le Al a Pr o Leu Asn 215 Al a Al a Val Al a Al a 295 I le G u Al a Ar g G u 375 Gy G n Asn G u 4et 455 Gy Tyr Al a Phe 200 Al a Val G u Gy Val 280 vet Leu Thr Tyr Asp 360 Pr o Thr Asp Phe SIle 440 Pr o Gy Al a Asp 185 Val Ar g Al a Phe Gy 265 I le Asp bet Hi s Leu 345 Gy Gy Ar g Leu Phe 425 Al a Gy Ser Thr 170 Leu SIle Al a Lys Gy 250 Ar g Gy G u Al a Al a 330 G n Ser Thr Leu Pr o 410 Al a Al a Asn Asp 155 Al a Val Al a Al a Hi s 235 I le Tyr Pr o Hi s Leu 315 Val G n Pr o Asn Val 395 Al a G n G u Ar g Gy l e Val Lys 205 Al a Val Thr Val Asp 285 Ar g Gy Pr o Thr Ser 365 G n Al a G u Lys Val 445 Thr Pr o Ser Leu 190 Thr Tr p Al a Asp Asp 270 Phe Thr Val Tyr et 350 Thr Hi s Asp Ar g Val 430 Al a Thr Al a Al a 175 G u Phe Leu Val Asn 255 Ser vet Thr Tr p Ser 335 G u Sy Al a Phe Thr 415 Leu Pr o Thr Leu Ala u u Leu Thr Pro Ser Ile Leu G v 465 475 Ala Leu lie Ala Leu 480 Page 57 ene Sequence 24- 2- 06. txt Tyr Ou His IlieVal I'bt Val On Gy Val i~e Trp Asp IlieAsn Ser 485 490 495 Phe Asp (1n Tr p 1y Val O31u Leu (1y Lys (1n (1nAla Asn Asp Leu 500 505 510 Al aPro Ala Val Ser O31y (1u 1u Asp Val Asp Ser 31y Asp Ser Ser 515 520 525 Thr Asp Ser Leu Ilie Lys Tr p Tyr Ar g Al a Asn Arg 530 535 540 <210> 43 <211> 630 <212> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 ODS <222> (630) <223> RXA01989 <400> 43 gt t ValI g ac As p tt t Ph e gt t ValI gca Al a g aa (31u t ta Le u aaa c aa O31n tt t Ph e 145 aac As n t ca Ser t ta Le u ag a Ar g gt a ValI g aa O31u gt a ValI g at As p ggt G31y 115 gt t ValI ac a Thr ggt G31y c ac Hi s t gg Tr p gt t ValI at c I Ie t ta Le u gt t ValI t ta Le u act Thr g aa O31u g at As p g aa O31u 1 65 aaa Ly s gt t ValI gaa O31u ggt G31y ac g Thr 70 ggt G31y gca Al a aca Thr ag a Ar g aaa Lys5 150 ac g Thr ac a Thr g at As p gca Al a at t I Ie t ca Ser aat As n g ac As p g aa O31u t ac Tyr 135 g aa O31u tt t Ph e at t I Ie tt a Le u t ca Ser 40 ggt G31y t ca Ser c ac Hi s aaa Lys5 cca Pr o 120 ggt G31y aaa Lys5 at c I Ie cat Hi s cca Pr o aaa Lys5 ggt G31y Ph e tt a Le u g at As p 1 05 gca Al a aaa Ly s ggt G31y gt a ValI g aa G u 10 gt t ValI cgc Ar g t ct Ser ag a Ar g t ca Ser 90 tt c Ph e gt t ValI g aa 0Ou gct Al a cct Pr o 1 70 ggt G y g at As p at t I Ie t ac Tyr aac As n 75 t ca Ser t ct Ser gca Al a g aa 0Ou tt a Le u 1 55 g at As p act Thr t ac Tyr aaa Ly s tt a Le u ag c Ser ac a Thr gt a ValI tt t Ph e gca Al a 140 aaa Ly s g at As p ggt G y g ac As p g aa 0Ou ggt 0Gy aat As n t at Tyr aac As n ag a Ar g 125 c aa 0On c ag 0On gt a ValI gca Al a aaa Ly s aat As n gca Al a g aa O31u ac g Thr gt t ValI 110 tt g Le u aaa Ly s tt g Le u ggt (31y ggt G y g aa 0Ou t ct Ser cgt Ar g t ac Tyr aaa Ly s at t I Ie tt c Ph e cgt Ar g gct Al a gga 0Oy 1 agt Ser g aa O31u g at As p gca Al a cct Pr a gag O31u t ct Ser aaa Ly s at a I Ie ac a Th r 1 ag a Ar g Page 58 ene Sequence 24- 2- 06. t xt t at t ct gt t t ta aca gca gta gga t ta t tacca at t gca aca gct gga Tyr Ser Val Leu Thr Al a Val G31y Leu Leu Pro IlieAla Thr Al a G31y 180 185 190 at t aac at c gaa gct at g at g at t ggt gct gca aaa gca cgt gaa gaa Ile Asn Ile (1u Ala /t N/t Ile (1y Al aAl aLys Al aAr g (1u 1u 195 200 205 t t a t ct Leu Ser 210 <210> 44 <211> 210 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm curn <400> 44 Val Lys Ser Asp Phe Leu Phe Ser Ar g Val Leu Val Al a IlIe G3 u G3 u IlIe Val Leu Val Asp Lys Ser G3 y 115 G3 n Leu Val 130 Phe Al a Thr 145 Asna1ua(1y Tyr Ser Val I e Hi s a31y Tr p 1I e Val Val I Ie I'b t Leu Phe Val Tyr Leu 1 00 Thr Thr a31ua(1u Thr As p Ty r a3 u 1 65 Leu Thr 1 80 Lys Thr Ilie His au 10 Val Asp Leu Pr o Val 25 G u Al a Se r Lys Ar g 40 a3 y I I e a3 y a3 y Se r Thr Ser Ser Phe Ar g 70 a3 y As n Hi s Leu Ser 90 Al a As p Lys Asp Phe 1 05 Thr a31u Pr o Al a Val 120 Ar g Ty r a31y Lys a31u 135 Lys a3 u Lys a3 y Al a 150 Thr Phe I Ie Val Pr o 1 70 Al a Val a31y Leu Leu 1 85 a31y As p I Ie Tyr As n Ser Ser Al a a31u Le u 1 As p Pr o a31y As p G u a31y As n Tyr As n Ar g 125 a31n a31n ValI Al a Al a Lys5 As n Al a a31u Thr ValI 110 Le u Lys5 Le u a31y Thr 1 a31y a31u Ser Ar g Tyr lie Ph e Ar g Al a a31y 1 Al a Ile Asn IleOu Ala I'bt I'bt IleOy AlaAla Lys AlaArgOuaOu 195 200 Leu Ser 210 <210O> <21 1> 1269 <212> DNA <21 3> Cor ynebact er i urn gi ut anm curn <220> 205 Page 59 Gene Sequence 24-2-06.txt <221> CDS <222> (101)..(1246) <223> RXA00340 <400> cggtatctcc gacatccgca acacccccaa agatgaggtt ctct t acctc atcactgaca tctct t agaa agaccaccca ccacagtgcc cagaatgtgg gag G u acc Thr t at Tyr ct a Leu gat Asp tgg Tr p gag G u gag G u gca Al a tct Ser 150 acc Thr cgt Ar g ctt Leu caa G n gac Asp gt g Val gt c Val gaa G u t ac Tyr at c SIle gct Al a aac Asn 120 gca Al a gct Al a acc Thr ttg Leu t cc Ser 200 gca Al a ggc Gy t cc Ser aca Thr ct c Leu gac Asp cac Hi s gaa G u cga Ar g aag Lys tcg Ser ccc Pr o 170 ctt Leu agg Ar g gca Al a t cc Ser gac Asp aaa Lys aaa Lys t cc Ser 75 ccc Pr o at c SIle gct Al a ccg Pr o gtt Val 155 acc Thr cgc Ar g t cc Ser gca Al a cgc Ar g aac Asn gcc Al a gca Al a aaa Lys gac Asp ggg G y gac Asp gt a Val 140 gcg Al a aac Asn cac Hi s aac Asn cga Ar g 220 gga Gy aaa Lys acc Thr 45 gcc Al a ctt Leu at g bet t cc Ser gca Al a 125 ggt G y ggc Gy tgg Tr p ggc Gy cca Pr o 205 cga Ar g aac Asn gca Al a aac Asn cgc Ar g gt c Val aaa Lys gtt Val 110 ttg Leu gtt Val t ca Ser aac Asn caa G n 190 gag G u ct c Leu ccc Pr o 15 gaa G u aac Asn gag G u gtt Val gtt Val 95 tct Ser t cc Ser gt a Val gag G u ggc Gy 175 act Thr ct g Leu gct Al a ggc Gy gtt Val gt c Val ct c Leu gaa G u 80 ct a Leu t at Tyr aac Asn ggg G y aaa Lys 160 gca Al a gct Al a tct Ser caa G n gt g Val 1 gt c Val ct c Leu gcc Al a ggc Gy caa G n gcg Al a acg Thr gt g Val gat Asp 145 gaa G u acc Thr at g Net gaa G u acc Thr 225 aaa tta gtc atc Lys Leu Val lle gcc ggc tcc ggc Al a G y Ser G y aaa gaa atc gcc Lys G u Ile Ala gaa tac cgc gga G u Tyr Arg y gct acc tcc gtg Ala Thr Ser Val atg tcc ggc cgg IVet Ser G y Arg at c gaa gcc aag lie G u Al a Lys 100 tgg att ccg cgt Trp Ile Pro Arg 115 gcg atg gat gct Al a IVet Asp Al a 130 tct gct tct gta Ser Al a Ser Val gac ctc aac tgc Asp Leu Asn Cys 165 aca gat ccc act Thr Asp Pro Thr 180 tca gtg gca cgc Ser Val Ala Arg 195 ctt ggt gaa aaa Leu G y G u Lys 210 ggt ggc atc gac G y G y Ile Asp gct att gtg agt tct ccg ctc acc cgc acg atg caa acc gca gaa gca Page Ala lie Val Ser Ser Pro Leu 230 235 Gene Sequence 24-2-06.txt Thr Arg Thr Met n Thr Ala u Ala gca Al a gaa G u gaa G u gcc Al a aag Lys 310 gt g Val gac Asp ct g Leu gcc Al a gac Asp gat Asp 280 ccc Pr o cgt Ar g agc Ser ggc G y at c SIle 360 ct g Leu 250 gga Gy gaa G u ggt Gy agc Ser gt c Val 330 t cc Ser gag G u gga G y ct g Leu ct g Leu gag G u ct c Leu 315 acc Thr tt c Phe ttt Phe at g et tgg Tr p cac Hi s t cc Ser 300 caa G n cca Pr o ttt Phe t ac Tyr aaa Lys gat Asp acc Thr 285 ct g Leu cgc Ar g at c SIle cag G n gaa G u 365 gt a Val gga Gy 270 aag Lys cag G n gaa G u aaa Lys aag Lys 350 gac Asp cgt Ar g 255 aaa Lys tgg Tr p acg Thr t ac Tyr gcc Al a 335 gca Al a ggc G y 240 gtt Val t ca Ser ct c Leu gtt Val ggt Gy 320 at c l Ie cac Hi s cca Pr o at c SIle ttt Phe act Thr aat Asn 305 gca Al a at g Net ctt Leu acc Thr gat Asp t ca Ser gac Asp 290 cga Ar g gcg Al a agg Ar g gac Asp tgc Cys 370 gat Asp gaa G u 275 t ca Ser cgt Ar g aat Asn caa G n ttg Leu 355 gt a Val ct c Leu 260 gcc Al a tct Ser gt g Val gtt Val gca Al a 340 gcg Al a aga Ar g 883 931 979 1027 1075 1123 1171 1219 1266 1269 ttc aac gac acc tca cac ctg gaa gcg Phe Asn Asp Thr Ser His Leu G u Ala 375 380 ct c <210> 46 <211> 382 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 46 Val Lys Leu Val Ile u Ala Asp Gy 1 5 Val Ala y Ser y Thr Val Val Tyr 25 Leu Lys G u I e Ala Tyr Val Val y Ala u Tyr Arg y Leu Leu u y 55 Sy Ala Thr Ser Val Asp Val Tyr Mbet 70 G n IVet Ser y Arg Trp Lys Ile Lys Ala lie G u Ala Lys G u Ile Ala Ser 100 105 tgacgacagt ctgacggaag G y Ser 10 Ser Asp Thr Lys Leu Lys Asp Ser His Pr o 90 G u Ile Page 61 Ar g Asn Al a Al a Lys Asp Gy Asn Al a Asn Ar g Val Lys Val 110 Pr o G u Asn G u Val Val Ser Gene Sequence 24-2-06.txt Thr Val Asp 145 G u Thr let G u Thr 225 G n SIle Phe Thr Asn 305 Al a bet Leu Thr SIle 115 Met Al a Leu Asp Val 195 Gy Gy Al a Asp G u 275 Ser Ar g Asn G n Leu 355 Val Pro Arg G u Lys Asn Lys Arg Ala Asp Ala Leu Ser Asn Al a Val Cys 165 Thr Ar g Lys Asp Al a 245 I le Hi s Val Lys Leu 325 Leu Ser Leu Al a Ser 150 Thr Ar g Leu G n Al a 230 Al a G u G u Al a Lys 310 Val Asp Leu Phe Al a 135 Ser G u Phe Tyr Al a 215 l le Al a Thr G n Pr o 295 Al a Val Al a Ser Asn 375 120 Al a Al a Thr Leu Ser 200 Al a Val Al a Asp Asp 280 Pr o Ar g Ser Gy I e 360 Asp 125 Gy Ser Lys Leu 185 Gy Al a Ser Al a Phe 265 Pr o Gy G u Hi s Pr o 345 Al a Thr Lys Ser Pr o 170 Leu Ar g Al a Ser Leu 250 Gy G u Gy Ser Val 330 Ser G u Ser Pr o Val 155 Thr Ar g Ser Al a Pr o 235 Gy Leu Leu G u Leu 315 Thr Phe Phe Hi s Gy Gy Tr p Gy Pr o 205 Ar g Thr Lys Asp Thr 285 Leu Ar g SIle G n G u 365 G u Val G u Gy 175 Thr Leu Al a Thr Ar g 255 Lys Tr p Thr Tyr Al a 335 Al a Gy Gy Lys 160 Al a Al a Ser G n et 240 Val Ser Leu Val Gy 320 l e Hi s Pr o <210> 47 <211> 840 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(817) <223> RXA02492 <400> 47 gctgtacaac gacgctattg ccaacgaaaa tgtcgacggt gaaacgcatc acggctaagt aaacgcgcgt cgtggaacat aaagtggcaa actagtacct atg act aac gga aaa 115 IVet Thr Asn G y Lys Page 62 Gene Sequence 24-2-06.txt ttg att ctt Leu IIe Leu ttc act gga Phe Thr G y gcc aaa ggc Al a Lys G y gcg atc cgc Al a lIe Ar g at c cca gt g lie Pro Val ctg cag ggc Leu G n G y cag ttc atg G n Phe Met gcg gat gac Al a Asp Asp 120 ct c gac gt a Leu Asp Val 135 ttt gtt cct Phe Val Pro 150 gaa acc gt c G u Thr Val aag cac ctt Lys His Leu cca acc ggc Pro Thr G y 200 gta aac cca Val Asn Pro 215 gca gca gca Al a Al a Al a 230 ct c <210> 48 <211> 239 <212> PRT ctt cgt cac Leu Arg Hi s tgg gtc gac Trp Val Asp gtc ctc cca Val Leu Pro act gca aac Thr Al a Asn atc cgc gac lie Arg Asp 75 ctt gac aag Leu Asp Lys gaa tgg cgc G u Trp Ar g 105 gca gag tac Al a G u Tyr gtt cca cgc Val Pro Ar g tac ttc gag Tyr Phe G u 155 ctc atc gca Leu Ile Al a 170 gac ggc at c Asp G y Ile 185 at c cca ct g Ile Pro Leu ggc ggc acc Sy y Thr gta gca aac Val Al a Asn 235 ggt G y gt c Val ggc Sy at c I e 60 tgg Tr p gct Al a cgc Ar g t cc Ser acc Thr 140 gaa G u gca Al a t cc Ser gt c Val t ac Tyr 220 cag G n aat Asn gtt Val 45 gca Al a cgc Ar g gca Al a t cc Ser cag G n 125 gaa G u gaa G u cac Hi s gat Asp t ac Tyr 205 ct c Leu agc Ser ct g Leu gt a Val ct g Leu ct c Leu acc Thr t ac Tyr 110 gca Al a tgc Cys at c SIle ggc G y gct Al a 190 gaa G u gat Asp 1 gaa tgg aac Su Trp Asn 15 acc gaa cag Thr G u G n t ac acc t cc Tyr Thr Ser aac gct gca Asn Al a Al a aac gag cgt Asn u Arg 80 aag gaa aaa Lys G u Lys 95 gac acc cca Asp Thr Pro aat gac cct Asn Asp Pro ctc aag gac Leu Lys Asp 145 ct g cca cgc Leu Pro Ar g 160 aac t cc ct g Asn Ser Leu 175 gat at c gca Asp Ile Al a atc gcc gaa Ile Ala G u cct gag gca Pro G u Ala 225 gca Al a ggt Sy ttg Leu gac Asp cac Hi s t ac Tyr cca Pr o cgt Ar g 130 gtt Val gca Al a cgt Ar g gag G u gac Asp 210 gca Al a aac Asn gct Al a cgt Ar g cac Hi s ggc Sy gac Asp 100 gag G u gcg Al a gtt Val aag Lys ct g Leu 180 aac Asn t cc Ser gcc Al a cag G n gag G u cgc Ar g tgg Tr p gca Al a gac Asp ct c Leu gac Asp cgt Ar g ggc G y 165 gtt Val at c SIe gta Val ggc Sy cag ggt aat aag Sn G y Asn Lys tagctatttg taggtgagca Page 63 Gene Sequence 24-2-06.txt <213> Corynebacter i um gl ut am cum <400> 48 IVet Thr Asn G y Lys Leu II e Leu Leu 1 Asn G n Ser Al a Ar g Lys Pr o Pr o Asp 145 Ar g Leu Al a G u Al a 225 Ser G u Leu Ar g Tyr Sy Pr o 115 Tyr Val Lys Al a Leu 195 G y Al a 5 G n G u Ar g Tr p Al a Asp Leu Asp Ar g Sy 165 Val SIle Val Phe Al a Al a SIle 70 Leu G n Al a Leu Phe 150 G u Lys Pr o Val Thr Lys l Ie Pr o G n Phe Asp Asp 135 Val Thr Hi s Thr Asn 215 Tr p Val Thr SIle Leu G u 105 Al a Val Tyr Leu Asp 185 I le Sy Hi s Asp Pr o Asn Asp Lys Ar g Tyr Ar g G u 155 Al a SIle Leu Thr G n Asn Val Al a Ar g Al a Ser G n 125 G u G u Hi s Asp Tyr 205 Leu Ser Leu Val Leu Leu Thr Tyr 110 Al a Cys SIle Sy Al a 190 G u Asp G u Thr Tyr Asn Asn Lys Asp Asn Leu Leu Asn 175 Asp SIe Pr o Ala O y Ala Ala Ala Val Ala Asn Gn Oy Asn Lys <210> 49 <211> 729 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(706) <223> RXA00381 <400> 49 aaacctggtt tgtgtcttcc gctctcacgg acgatgattt ctccaagatc gagcaggcac tcaagcccgc cgcacgtgca gcagcagaag cgaaggcatc atg acg caa acc att 115 et Thr Gn Thr lie 1 gtc cat cta gtt cgc cac ggc gaa gtc cac aac cca gag aaa atc ctg 163 Val His Leu Val Arg His O y O u Val His Asn Pro O u Lys Ile Leu Page 64 Gene Sequence 24-2-06.txt t ac Tyr gcc Al a gcg Al a aag Lys ggc Sy tgg Tr p tgg Tr p gaa G u cac Hi s 150 ct g Leu t cc Ser cca Pr o cgc atg ccc Arg IVet Pro cgc act gca Arg Thr Ala tcc cca ttg Ser Pro Leu aca ggc ct a Thr G y Leu cgt ttc gaa Arg Phe G u ccc gtg cgt Pro Val Arg 105 gaa cac tac G u Hi s Tyr 120 gct cgg gt g Al a Ar g Val ttg ccg atc Leu Pro Ile cat aac cca His Asn Pr o 170 gtg ttc caa Val Phe G n 185 cag gag att G n lu lie 200 gga G y gct Al a cag G n gaa G u 75 ggc Sy tgg Tr p acc Thr gca Al a gt g Val 155 gcg Al a gac Asp t ac Tyr tct Ser cgt Ar g ct g Leu ct g Leu cct Pr o gac Asp gcg Al a 140 tgc Cys acc Thr gat Asp agg Ar g ttt Phe 45 gt g Val at c SIle cgc Ar g ttg Leu at t SIle 125 gaa G u gt g Val agg Ar g caa G n ttg Leu 30 gaa G u cag G n acc Thr acc Thr at g bet 110 ttg Leu gga G y caa G n caa G n at t SIle t cc Ser cac Hi s acc Thr gag G u 80 ggt Sy aac Asn aga Ar g gaa G u cac Hi s 160 gac Asp ggc G y cgt Ar g gat Asp t cc Ser gat Asp tgg Tr p ccc Pr o at g Net gca Al a 145 gcc Al a ct c Leu gt g Val gga gt c Val gaa G u ctt Leu cgt Ar g acg Thr at g Net 130 at c l Ie cgc Ar g gcc Al a cat Hi s cgc Ar g acc Thr ccg Pr o ct g Leu t cc Ser ctt Leu 115 gcg Al a ct g Leu gga G y t ca Ser t ac Tyr 195 agc Ser t ac Tyr ttc Phe gaa G u cag G n 100 ccc Pr o gct Al a gt g Val caa G n gt g Val 180 aac Asn caa G n att SIe at c SIe gca Al a ttg Leu agc Ser gtg Val acc Thr agc Ser 165 aca Thr gaa G u tgatcactcg tgcgtttgac caa <210> <211> 202 <212> PRT <213> Corynebacter i um gl ut ari cum <400> Net Thr G n Thr lie Val His Leu Val Arg His G y G u Val His Asn 1 5 10 Pro G u Lys lie Leu Tyr Oy Arg IVet Pro y Tyr Arg Leu Ser Ser 25 Arg G y Arg Ser G n A a Ala Arg Thr Ala Ala Ser Phe G u G y His 40 Asp Val Thr Tyr Ile Al a Ala Ser Pro Leu G n Arg Val n G u Thr 55 Page Ser Asp Tr p Pr o Met Al a 145 Al a Leu Val Pr o Leu Ser Leu 115 Al a Leu Sy Ser Tyr 195 SIle Al a Leu Ser Val Thr Ser 165 Thr G u Val Asn Asn Sy Ar g 135 G n Ser Leu Al a Gene Sequence 24-2-06.txt Thr G y Leu G u Leu I e Thr Arg Phe G u y Leu Arg Thr 90 Pro Val Arg Trp Pro Leu IVet 105 110 G u His Tyr Thr Asp Ile Leu 120 125 Ala Arg Val Ala Ala G u y 140 Leu Pro lie Val Cys Val G n 155 His Asn Pro Ala Thr Arg G n 170 Val Phe n Asp Asp n Ile 185 190 Gn Gu lie 200 Asp Lys Tyr G u Hi s Ar g Cys 175 Val <210> 51 <211> 822 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(799) <223> RXA02122 <400> 51 ggccttcagc tcaccgacag tttgtacatt tggtggaaga ccttttttta agtgggcggt caggaatttt tcgcacaggt aag Lys tta Leu ggt Sy cgg Ar g t ca Ser gct Al a ggt Sy at t SIle acg Thr gt g Val ccg Pr o ggc G y cac Hi s cat Hi s ggt Sy cag G n gag G u gag G u gca Al a ggg Sy tta Leu cgt Ar g cgt Ar g 75 tt g Leu gct Al a caa G n cac Hi s ct g Leu gcg Al a acg Thr gcc Al a acc Thr ct g Leu 45 gcg Al a cag G n gt g Val gaa G u cca Pr o tct Ser gag G u gaa G u gaa G u aag act Lys Thr 15 aca act Thr Thr gat aag Asp Lys gtg ccg Val Pr o aca gca Thr Al a 80 cct ggg Pro G y Page 66 ctcacacccc acaccctaga atg ctg cat gtc atg Net Leu His Val bet 1 caa tcc act gtg gtt G n Ser Thr Val Val ggt cag gtt ctg cct Sy G n Val Leu Pro ggt gaa gag cag gcg G y u u G n Ala att acc gct gtg tat Ile Thr Ala Val Tyr gca ccg acg gtc agc Ala Pro Thr Val Ser ctt att gaa tgc gat Leu Ile G u Cys Asp Gene Sequence 24-2-06.txt ttc ggc gag tgg acg ggc Phe Gy G u Trp Thr G y 105 gag tgg aaa gcg gtg cag G u Trp Lys Ala Val G n 120 ggt gag agt ttc gtg gaa Gy u Ser Phe Val G u 135 aac att gcg cag cag cat Asn Ile Ala Gn Gn His 150 155 gcc gac acg atc aag gct Ala Asp Thr Ile Lys Ala 170 gat tct ttt cag cgc att Asp Ser Phe G n Arg Ile 185 gaa ttt acc ggg aaa tct G u Phe Thr G y Lys Ser 200 aat tcc aga aca gga tcg Asn Ser Arg Thr y Ser 215 gct ccg caa cca tgatcacc Ala Pro G n Pro 230 <210> 52 <211> 233 <212> PRT <213> Corynebacter i um gl <400> 52 Met Leu His Val et Lys 1 5 G n Ser Thr Val Val Leu Sy G n Val Leu Pro G y G y u u G n Ala Arg Ile Thr Ala Val Tyr Ser 70 Ala Pro Thr Val Ser Ala Leu Ile G u Cys Asp Phe 100 Leu Asn Al a Leu G u G u 115 cgg Ar g aag Lys at g bet 140 ccg Pr o gcg Al a ttc Phe t ca Ser ttg Leu 220 aaa Lys aca Thr 125 cag Gn gga Sy gt g Val at c SIle ggc G y 205 gga G y ct a Leu 110 ccg Pr o gat Asp gaa G u gct Al a gac Asp 190 gt c Val t ac Tyr act gag Thr G u tct acc Ser Thr cgg at g Arg IVet atc gtt Ile Val 160 cat ttt Hi s Phe 175 acg gcg Thr Al a t cc t cc Ser Ser ctt cga Leu Arg ct c aat Leu Asn ttc agg Phe Arg 130 gtg gag Val G u 145 gct gcg Al a Al a gta ggc Val G y tca at t Ser Ile cat at g Hi s bet 210 gac aaa Asp Lys 225 gcc Al a 115 ttt Phe gct Al a ttt Phe act Thr t cc Ser 195 ct g Leu ctt Leu ct a Leu cca Pr o at c l Ie agt Ser cca Pr o 180 gca Al a ct g Leu ccg Pr o gag G u ggt Sy ggc Gy cat Hi s 165 ctg Leu gtg Val aca Thr aaa Lys tc accatttgag cgc ut am cum Pro G y Ser His Al 10 Leu lie Arg His G G n Thr Pro y L 40 G u Val Ala G n Ar 55 Ser Pro Met G u Ar Hi s G y Leu G u L 90 Sy u Trp Thr G 105 Trp Lys Ala Val G 120 Page a y eu rg rg 75 eu y n 67 Al a G n Hi s Leu Al a Thr Ar g Lys Al a Thr Leu Al a G n Val Lys Thr 125 Lys Thr Asp Val Thr Pr o Thr Ser Thr Thr Lys Pr o Al a Sy G u Thr ene Sequence 24 Phe Arg Phe Pro Gy Gy Ou Ser Phe Val (31u 130 135 Val 31u AlaIlIe (1y Asn IlIe Ala (1n (1n Hi s 145 150 155 Ala Ala Phe Ser Hi s Ala Asp Thr Ilie Lys Ala 165 170 Val O31y Thr Pr o Leu Asp Ser Phe O31n Ar g Ilie 180 185 Ser Ilie Ser Ala Val O31u Phe Thr G31y Lys Ser 195 200 Hi s I'bt Leu Leu Thr Asn Ser Ar g Thr G31y Ser 210 215 Asp Lys Leu Pr o Lys Ala Pro O31n Pr o 225 230 <210> 53 <211> 1161 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CDS <222> (1138) <223> RXA00206 <400> 53 t taaat aaga t ggt cagaga cagtt tt t tg gcct gt caac t gggt gat tg t tccggcgcg ggtgt tgt ga tgggt t taat 2- 06. t xt [vb t O31nAs p 140 Pr o 31y O31u Ala Val Ala Phe I I e As p 1 Ser 03 y Val 205 Leu 03 y Ty r 220 Ar g I Ie Hi s 1 Thr Ser Le u ccct gt gat t ct ct tat t tt at t I Ie at c I Ie gt t ValI c ag O31n ac c Thr at t I Ie at c I Ie act Thr gga G31y t at Tyr t at Tyr tt g Le u c ag O31n at c I Ie ac g Thr gt c ValI g ac As p g at As p act Thr aag Lys5 ggc (31y t ca Ser cgc Ar g ggt O31y gaa O31u ggt O31y 75 gcc A a gaa (31u ggc O31y ac a Thr t gg Tr p g at As p cgc Ar g aac As n gga (31y ggc O31y gcc A a g aa O31u 45 at t I Ie ct c Le u tt a Le u ac c Thr g ac As p ag c Ser gga O31y g ac As p cat Hi s g aa O31u ct g Le u 110 t gc cys 15 aat As n ct g Le u cga Ar g ccg Pr o g ac As p 95 aag Lys5 ccc Pr o g aa O31u tt a Le u at c I Ie g ac As p 80 gcc A a ggt (31y at g vb t 1 gga O31y tt t Ph e ggc O31y ct c Le u aag Lys5 O31y gcc A a ct a Le u ggc O31y g at As p ct t Le u tt t Ph e at c I Ie aag Lys5 aac As n t cc Ser cgt Ar g cga Ar g aag Lys5 g at As p t gg Tr p 115 gcc A a ac c Thr cgc Ar g ggc O31y gcc A a gcc A a 1 00 ct g Le u gaa gac atg 31u As p b t cga Ar g gt c ValI gt c ValI gt a ValI O31y gga O31y ct t Le u t ct Se r Page 68 ene Sequence 24- 2- 06. txt gat aac ggt As pAs n (1y 120 gt g aat ggc Val As n (1y 135 gct acc gac A a Thr Asp 1 50 cgt gt g at g Ar g Val [Vb t ct g cac gca Leu Hi s A a gt a cct tt c Val Pr o Phe 200 cag at g ggc G n /b t G y 215 cca cgc gaa Pr o Ar g O31u 230 ggt cac aag a3 y Hi s Lys cac gt g cgc Hi s Val Ar g caa cgt ggt O31n Ar g G31y 280 t at ggt gt t Tyr G3 y Val 295 gt t gt t gct Val Val A a 310 gca gt c gga Ala Val G31y cag gca at g 03 n A a [Vb t at c cct gt t gt c Ilie Pro Val Val ggt gt c cca aag acc att gac aat gac Pro Lys Thr Ilie Asp Asn Asp g ac As p gt t ValI gt g ValI 1 70 at g vb t at t I Ie aag Ly s ac c Thr tt c Ph e 250 ggc G31y ac c Thr gca A a aag Lys5 tt g Le u 330 t t c Ph e gac As p 155 gag (31u gcc A a gca A a t ac Tyr at g vb t 235 ac g Thr cac Hi s cca Pr o gct A a ggt G31y 315 aag Lys5 ac c Thr 140 cgc Ar g gt c ValI G31y gag (31u G y 220 gag (31u gga G31y g at As p act Thr cgt Ar g 300 gag (31u g aa (31u U y 125 tt c Ph e ct g Le u at g vb t ggt G31y at c I Ie 205 at t I Ie ct t Le u at t I Ie gt t ValI gct A a 285 gcg A a ag c Ser gt t ValI ValI ggt (31y c ac Hi s ggc G31y gct A a 1 90 t gc cys at c I Ie cgt Ar g gga G31y cgt Ar g 270 tt c Ph e t gc Cys at t I Ie cca Pr o g at As p ac c Thr 1 60 c ac Hi s t ac Tyr gcg A a gt t ValI ggc G31y 240 c ag O31n ac c Thr cgt Ar g gag O31u at g vb t 320 g aa O31u act Thr 145 gct A a gt g ValI ac c Thr at g vb t gcg A a 225 c ac Hi s at c I Ie gt t ValI gt t ValI gga G31y 305 at c I Ie cgc Ar g 130 gct A a g aa O31u ggt G31y gt t ValI g aa O31u 210 g aa G u at t I Ie gct A a ct t Le u ct g Le u 290 ag c Ser ac c Thr t gg Tr p gt g ValI t ct Ser t gg Tr p at t I Ie 1 cgt Ar g ggt G y g ac As p g at As p ggc 0Gy 275 gcc A a tt t Ph e tt t Ph e gt t ValI gca A a c ac Hi s at t I Ie 1 cca Pr o cgc Ar g gcg A a c ag O31n gag (31u 260 c ac Hi s act Thr g ac As p g aa (31u act Thr 340 gt g ValI aac As n 1 gct A a g aa (31u tt c Ph e tt g Le u tt c Ph e 245 at c I Ie at t I Ie cgt Ar g aag g aa (31u 325 gcc A a 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1161 ttt gga Phe 03 y 345 t agt tt t tcg ggctt t tat c aac <210O> 54 <21 1> 346 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 54 I'b t O31uAsp I'bt Arg lieAla Thr Leu Thr Ser Gy Gy Asp Cys Pro Page 69 Gene Sequence 24-2-06.txt 1 5 10 Gy Leu Asn Ala Val I e Arg Gy I e Val Arg Thr Ala Ser Asn u 25 Phe G y Ser Thr Val Val G y Tyr G n Asp G y Trp G u G y Leu Leu 40 Sy Asp ArArgrg Val G n Leu Tyr Asp Asp u Asp Ile Asp Arg lie 55 Leu Leu Arg G y G y Thr lie Leu G y Thr Gy Arg Leu His Pro Asp 70 75 Lys Phe Lys Al a G y ile Asp G n Ile Lys Al a Asn Leu G u Asp Al a 90 Gy Ile Asp Ala Leu lie Pro lie Gy Gy u y Thr Leu Lys y 100 105 110 Ala Lys Trp Leu Ser Asp Asn Gy Ile Pro Val Val y Val Pro Lys 115 120 125 Thr Ile Asp Asn Asp Val Asn y Thr Asp Phe Thr Phe y Phe Asp 130 135 140 Thr Ala Val Ala Val Ala Thr Asp Ala Val Asp Arg Leu His Thr Thr 145 150 155 160 Ala u Ser His Asn Arg Val Vet IIe Val G u Val Vet G y Arg His 165 170 175 Val G y Trp IIe Ala Leu His Ala G y Vet Ala G y G y Ala His Tyr 180 185 190 Thr Val Ile Pro u Val Pro Phe Asp lie Ala u lie Cys Lys Ala 195 200 205 et u Arg Arg Phe n et Gy u Lys Tyr Gy Ile Ile Val Val 210 215 220 Ala G u G y Ala Leu Pro Arg G u G y Thr IVet G u Leu Arg G u G y 225 230 235 240 His IIe Asp G n Phe G y His Lys Thr Phe Thr G y IIe G y n G n 245 250 255 lie Ala Asp G u IIe His Val Arg Leu G y His Asp Val Arg Thr Thr 260 265 270 Val Leu G y His IIe G n Arg G y G y Thr Pro Thr Ala Phe Asp Arg 275 280 285 Val Leu Ala Thr Arg Tyr G y Val Arg Ala Ala Arg Ala Cys His G u 290 295 300 G y Ser Phe Asp Lys Val Val Al a Leu Lys G y u Ser Ile G u bet 305 310 315 320 Ile Thr Phe G u G u Al a Val y Thr Leu Lys G u Val Pro Phe G u 325 330 335 Arg Trp Val Thr Ala G n Ala et Phe y 340 345 <210> <211> 1083 Page Gene Sequence 24-2-06.txt <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(1060) <223> RXA01243 <400> gcgcaatcag cgatatcgat gtggtggtca ccgatgcggg tgcaccagca agtttcgttg agcagttgcg agaacgcgat gtagaagttg tgattgcaga act gca agt Thr Al a Ser gaa at t ggc G u lie y ggt ttt ggc G y Phe G y act ttt gca Thr Phe Al a gtg acg ttt Val Thr Phe atc ccc at g Ile Pro lMet ttc aaa gac Phe Lys Asp cgt gat ct a Arg Asp Leu 120 ggt ggc aat G y y Asn 135 gt g aga t ca Val Arg Ser 150 gca act ggt Al a Thr G y t cc ccg gat Ser Pro Asp gga tta gaa G y Leu G u 200 t at Tyr gca Al a ggt G y ttt Phe ggg G y ttg Leu ccc Pr o gt g Val ccg Pr o cgc Ar g gcg Al a 170 ct g Leu aaa Lys ttg Leu aaa Lys gct Al a gct Al a 60 cct Pr o at g et cct Pr o cga Ar g at c SIle 140 t ac Tyr cgt Ar g gt g Val ttg Leu agc Ser at c SIle gcc Al a 45 cca Pr o cat Hi s cgt Ar g ttg Leu gcc Al a 125 gcg Al a cac Hi s gcg Al a gct Al a aga Ar g 205 acc Thr cgg Ar g acc Thr gaa gaa G u gat Asp gat Asp 110 gaa G u cct Pr o cct Pr o gtt Val gcg Al a 190 ggt G y aat Asn 15 cag G n ttg Leu at c SIle at t SIle gca Al a 95 gt g Val gat Asp gct Al a cat Hi s at t SIle 175 gaa G u cca Pr o gag G u gtt Val ttt Phe tct Ser at t I e 80 gag G u t cc Ser gcc Al a gcg Al a gt g Val 160 cga Ar g gaa G u tgg Tr p at g bet 1 ctt Leu t cc Ser t at Tyr cat Hi s ccg Pr o ggc G y cag G n gcg Al a tgg Tr p 145 aag Lys cag G n at c SIle gt a Val gac Asp act Thr ggc G y t ac Tyr gt g Val aat Asn ttg Leu tgg Tr p 130 ttt Phe gt a Val ctt Leu gaa G u gag 10u 210 att ctt aca I e Leu Thr ggc G y gtt Val ggc G y at g let gca Al a gag G u gca Al a 115 gt g Val gt g Val gct Al a gca Al a at t SIle 195 gga G y acc Thr gcc Al a aat Asn cgc Ar g ggt G y act Thr 100 at t l Ie ttg Leu gat Asp at c l Ie gct Al a 180 gcc Al a gat Asp gtc Val at c SIe ggt G y gaa G u ctg Leu ccc Pr o aag Lys ctt Leu ttg Leu gtg Val gca Al a 165 acg Thr act Thr ct c Leu tcc ccg act gtg gcg gca gcg cgc gct tta att Page 71 gat agc ggt gtc acc Ser Pro Thr Val Ala Ala Ala Arg A 215 220 gag gtg ttg gtt acc aac aag cgg a G u Val Leu Val Thr Asn Lys Arg T 230 235 tct gaa tca ctg tta gcc agc tac g Ser G u Ser Leu Leu Al a Ser Tyr A 250 gtg aat tgg cgg gaa tct ttt act g Val Asn Trp Arg u Ser Phe Thr A 265 2 gat gga aaa tcc act gag gac agc g Asp G y Lys Ser Thr G u Asp Ser 280 285 aac gct gaa ggc agt gag tgg gac a Asn Al a G u G y Ser G u Trp Asp A 295 300 ctt cgg gcg gag cac gtg gtc atc a Leu Arg Ala G u His Val Val Ile L 310 315 aaa <210> 56 <211> 320 <212> PRT <213> Corynebacter i um gl ut am cum Sequence 24-2-06.txt I a Leu Ile Asp Ser y Val Thr 225 Icg gaa tct ttg tat gtt tcc gag 835 hr u Ser Leu Tyr Val Ser G u 240 245 iac agc acc cct ggt aag cag ggc 883 sp Ser Thr Pro y Lys G n y 255 260 ica gga ttc ttg gca gca tcc aat 931 l a G y Phe Leu Al a Al a Ser Asn 70 275 itg atc aac gcg gtt gct tac gcc 979 (al lie Asn Ala Val Ala Tyr Ala 290 iac tac att ccc aca ccc gat aag 1027 sn Tyr lie Pro Thr Pro Asp Lys 305 iaa tcg ctt tagaccacgc aaaaagcctc 1080 ys Ser Leu 320 1083 <400> 56 'e t Ile 1 Leu Asp Ser Thr Tyr G y Hi s Tyr Pro Val Sy Asn G n Leu Al a Tr p 130 Trp Phe 145 Leu Thr y Thr Val Ala Sy Asn Ibet Arg Al a G y G u Thr 100 Al a I e 115 Val Leu Val Asp Val 5 I le Sy G u Leu Pr o Lys Leu Leu Val Al a SIle Phe Phe 55 Thr Pr o Lys Asp Sy 135 Ar g Pr o G u Thr Val Al a Hi s Ser 105 Val Leu Leu Tyr 10 Al a Sy Phe Sy Leu 90 Pr o Val Pr o Ar g Leu Lys Al a Al a Pr o bet Pr o Ar g SIle 140 Tyr Ser SIle Al a Pr o Hi s Ar g Leu Al a 125 Al a Hi s Asn G n Leu SIle SIle Al a Val Asp Al a Hi s G u Val Phe Ser I e G u Ser Al a Al a Val 160 Lys Val Ala Ile Ala Ala Thr Gy Ala Ala Leu Arg Ala Val li e Arg 175 Page 72 G n SIle Val Asp 225 Leu Pr o Leu Al a Pr o 305 Al a SIle 195 Gy Gy Val Lys Al a 275 Al a Pr o Thr Thr Leu Thr G u 245 Gy Asn Al a Lys Pr o Leu Pr o 215 Val G u Asn Gy Al a 295 Ar g Gene Sequence 24-2-06.txt Asp Ala Leu Ile Val Ala Ala 185 190 G u Pro Lys Thr Leu Arg y 200 205 Thr Val Ala Ala Ala Arg Ala 220 Leu Val Thr Asn Lys Arg Thr 235 Ser Leu Leu Ala Ser Tyr Asp 250 Trp Arg u Ser Phe Thr Ala 265 270 Lys Ser Thr G u Asp Ser Val 280 285 Gu y Ser u Trp Asp Asn 300 Ala G u His Val Val Ile Lys 315 G u Pr o Leu G u Ser 255 Gy SIe Tyr Ser <210> 57 <211> 1113 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1090) <223> RXA01882 <400> 57 accctttttc caccttggct gattttgatg cccttgttac ttccagtttt gcccgaccac aactttcagg tggtaacccc acc Thr t cc Ser ggt Sy acc Thr gt c Val aac Asn gga G y ggc G y gct Al a gac Asp agt Ser gt c Val aat Asn ttc Phe ggc G y at t SIle caa G n gt c Val cca Pr o tt g Leu 75 gat Asp cga Ar g gcc Al a gcc Al a ccc Pr o acg Thr gat Asp gct Al a aag Lys gaa G u ctg tcg Leu Ser 15 t cc gt c Ser Val gtc ttg Val Leu ctc gac Leu Asp act gtt Thr Val 80 Page 73 cgatgaccac acgctagatt atg atc atc aca ttc IVet Ile Ile Thr Phe 1 ctc ggc gaa gag ctc Leu G y G u G u Leu acc gct gtc gca ggt Thr Ala Val Ala y ctt gcg ggc ttt gaa Leu Al a G y Phe G u ccc ttc gtc cca ctg Pro Phe Val Pro Leu gtg atc aac aag aac Val Ile Asn Lys Asn Gene Sequence 24-2-06.txt gt c Val ct c Leu gaa G u ct g Leu ct c Leu 150 gat Asp cct Pr o cag G n ggc Gy ggc Gy 230 gt c Val gt a Val gca Al a tct Ser cct Pr o 310 ttg Leu acc Thr ggc G y gt g Val 120 ggc G y gcg Al a t ca Ser gct Al a gct Al a 200 t ac Tyr gaa Gu gca Al a acc Thr t cc Ser 280 ggc G y caa G n gaa Gu aac acc Asn Thr ccc ggc Pro y 105 ctt atc Leu II e tcg ctg Ser Leu ttg atc Leu II e gac aag Asp Lys 170 gct ccg Ala Pr o 185 aac act Asn Thr gac gcc Asp Al a cag gtg G n Val gaa ggt G u G y 250 gtt gga Val G y 265 cag aag n Lys t cg act Ser Thr ctc gcc Leu Al a t ca gca Ser Al a 330 aca Thr gcg Al a gac Asp cca Pr o cat Hi s 155 cca Pr o aac Asn gat Asp at c l Ie ctt Leu 235 gcg Al a gct Al a aaa Lys gcg Al a aca Thr 315 gtc acc gaa ccg gac ggc acc Val Thr G u Pro Asp Gy Thr 95 ccg ctc agc gag cag aag ctc Pro Leu Ser G u G n Lys Leu 110 gcg ctc cgc ccc gaa gtc acc Ala Leu Arg Pro G u Val Thr 125 130 cca ggg gca cca gtt gac tgg Pro Gy Ala Pro Val Asp Trp 140 145 tca gca cgc cct gac gtt cgc Ser Ala Arg Pro Asp Val Arg 160 ctg atg gcg ttg ggc gag agc Leu IVet Ala Leu y G u Ser 175 ctg att aag cca aat ggt ctg Leu lie Lys Pro Asn y Leu 190 ggt gaa gag ctg gag gcg cgt G y G u G u Leu G u Ala Ar g 205 210 atc gca gct gcg gac gta ctg lie Ala Ala Ala Asp Val Leu 220 225 gtc acc ttg ggt gcc gca gga Val Thr Leu Gy Ala Ala y 240 tgg act gct act t ct cca aag Trp Thr Ala Thr Ser Pro Lys 255 gga gac tgt gct ctt gca ggt G y Asp Cys Ala Leu Ala G y 270 aca ctg gag gaa tct ctg ct g Thr Leu G u G u Ser Leu Leu 285 290 gcg tct ctt cct ggc act acc Ala Ser Leu Pro Gy Thr Thr 300 305 gct ggt gca acg gtc acc caa Ala G y Ala Thr Val Thr G n 320 acc acc aag Thr Thr Lys 100 cgt agc ttg Arg Ser Leu 115 tgg gtt gtc Trp Val Val tac gcg cgt Tyr Al a Arg gtg gct gtc Val Al a Val 165 ttg gat aca Leu Asp Thr 180 gaa ctg ggc G u Leu G y 195 gct gcg caa Al a Al a G n gtt aac cgt Val Asn Arg gcg gtg ttg Al a Val Leu 245 att gat gt t Ile Asp Val 260 ttt gtt atg Phe Val bet 275 aat gcc gt g Asn Al a Val att cct cgt I e Pro Arg gtc aaa gga Val Lys G y 325 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1113 tgaatagcgt aaataattcc tcg <210> 58 <211> 330 Page 74 Gene Sequence 24-2-06.txt <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 58 IVet Ile lie Thr 1 Leu Thr Leu Pr o Val Gy Lys Val Asp 145 Val G u Gy Al a Val 225 Al a Pr o Al a Leu Thr 305 Thr G u Val Gy Val Asn Thr Ar g 115 Tr p Tyr Val Leu Gu 195 Al a Val Al a SIle Phe 275 Asn SIle Val Phe Thr Pro 5 Leu Ser Arg G y G y Lys G u Thr Leu Leu Val Arg 70 Asn Val Arg Lys Leu Asn Leu G u Lys Val Leu Al a 135 Arg Leu Thr 150 Val Asp Thr 165 Thr Pro y G y G n Leu Sn y Asp 215 Arg y lle 230 Leu Val Asn 245 Val Val Ser IVet Al a Arg Val Ser Tyr 295 Arg Pro Asp 310 G y Leu Lys 325 Asn Sy Sy 40 Al a Asp Thr Sy Val 120 Sy Al a Ser Al a Al a 200 Tyr G u Al a Thr Ser 280 Sy G n G u Pr o Ser I le Val SIle Asn Pr o 105 Leu Ser Leu Asp Al a 185 Asn Asp G n G u Val 265 G n Ser Leu Ser SIle G n Val Pr o Leu Thr Al a Asp Pr o Hi s 155 Pr o Asn Asp SIle Leu 235 Al a Al a Lys Al a Thr 315 Asp Ar g Al a Al a Pr o Val Pr o Al a Pr o 140 Ser Leu Leu Gy I e 220 Val Tr p Sy Thr Al a 300 Al a Ser Leu Hi s Sy Val Thr Leu Leu 125 Sy Al a et SIle Gu 205 Al a Thr Thr Asp Leu 285 Ser Sy Thr Asp Al a Lys G u G u Ser 110 Ar g Al a Ar g Al a Lys 190 Gu Al a Leu Al a Cys 270 G u Leu Al a Leu Ser Val Leu Thr Pr o G u Pr o Pr o Pr o Leu 175 Pr o Leu Al a Sy Thr 255 Al a G u Pr o Thr Page ene Sequence 24- 2- 06. txt <210O> 59 <21 1> 1155 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1132) <223> RXA01702 <400> 59 at tgt gaat g t gggat tat c ggt tcgcgct t caccat gt t acat agt tca gt gacagt ca cctt t tggag gagacacct t t ct gcat gat gaaat tacat ccc Pr o gcc A a ct c Le u ac c Th r aag Ly s t ac Ty r ct g Le u g ac As p gct A a aag Ly s 1 50 ggt G31y ac c Th r gag (31u gt c ValI cca Pr o G31y ggt G31y gca A a at c I Ie gag O31u ggc G31y 120 cca Pr o aag Ly s g aa O31u cca Pr o ggc G31y 200 aac As n at c I Ie gca A a gag O31u gcg A a gt t ValI gt c ValI ct t Le u g ac As p gcg A a g ac As p 1 70 g ac As p t ac Ty r gag O31u aac As n gag O31u t t c Ph e ct t Le u 75 gct A a cgc Ar g cca Pr a gag O31u aac As n 155 ggc G31y tt t Ph e ct g Le u at g vb t t gc cys gct A a ggt G31y gca A a ct g Le u cca Pr a ct g Le u aac As n 140 at c I Ie gt t ValI gag O31u ct a Le u ct c Le u ac c Th r g aa O31u 45 t cc Ser gcc A a c ac Hi s ct g Le u t tc Ph e 125 ct c Le u at c I Ie gag O31u aag Ly s gca A a 205 g at As p t cc Ser t ct Ser ggc G31y Ph e act Th r ct g Le u 110 c ag O31n g aa (31u tt g Le u gct A a ac c Th r 1 gct A a gct A a g aa (31u gga G31y gca A a c ac Hi s c ac Hi s at c I Ie c ac Hi s gca A a gt t ValI 1 gct A a g at As p tt c Ph e at g vb t 1 aag Ly s ac c Th r at c I Ie gt a ValI gag (31u t gc cys t cc Ser at g vb t c ag O31n 145 gag O31u G31y gca A a ggt (31y g aa O31u at c I Ie at c lIe aag Ly s gca A a c ag O31n c ag O31n t gg Tr p 130 gag O31u at c I Ie gca A a at c I Ie aac As n 210 cct at c gca Pr o I Ie Ala gga O31y gca A a tt c Ph e aag Ly s aag Ly s g aa O31u 1 00 cgc Ar g ggt O31y ct g Le u gt t ValI ct c Le u 1 ac c Th r c ac Hi s Page 76 Gene Sequence 24-2-06.txt gtt Val ggc G y 230 cca Pr o at c SIle gac Asp aac Asn gct Al a 310 gag G u acc Thr aag Lys cag G n gac Asp gag G u cag G n 280 aac Asn gac Asp at t SIle aag Lys cca ggc Pro G y gtt gca Val Ala ttc gtc Phe Val 250 gcg ctg Al a Leu 265 tac gca Tyr Al a ggc gtt G y Val cca cgc Pro Ar g atc gag Ile u 330 aac gt c Asn Val 220 cgc aag Arg Lys 235 ttc cac Phe Hi s acc tac Thr Tyr ttc acc Phe Thr ctc aag Leu Lys 300 tct tac Ser Tyr 315 tct tgc aag ctg cgc cca Lys Leu Arg Pro ctt Leu ggc G y gt c Val 270 cca Pr o gac Asp aag Lys gac Asp gga G y t ca Ser 255 at c SIle at c SIle ggc G y aag Lys ct c Leu 335 ctt Leu 240 ggc G y aag Lys gt c Val gag G u gct Al a 320 aag Lys gag gtc ctc ctt gag G u Val Leu Leu G u 225 gca gac gac gca ctt Ala Asp Asp Ala Leu 245 tcc gag aag gaa aag Ser G u Lys G u Lys 260 atg aac gtt gat act IVet Asn Val Asp Thr 275 tcc cac atg ttt gag Ser His et Phe G u 290 gtc gga aac aag aag Val G y Asn Lys Lys 305 gag cag agc atg tct G u G n Ser et Ser 325 tct gtt gga aag acc Ser Val G y Lys Thr 340 787 835 883 931 979 1027 1075 1123 1155 Ser Cys G n taatctcagc agttaaaaag ggc <210> <211> 344 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> IVet Pro Ile Ala Thr Pro u Val Tyr 1 5 Lys u Gy Gy Phe Ala Phe Pro Ala Thr I e Asn Ala Ala Leu Lys y Phe Ile I e G n Phe Ser Thr G y G y Al a 55 Val Lys Asn Lys Val Lys G y Al a Val 70 G u Al a Al a Lys Ser Tyr G y I e Asn Cys G n Lys G u Val Leu Asp G u Tyr 100 105 Ser n u Arg Val Asp Arg Gy u 115 120 Asn G u IVet 10 I e Asn Cys Ala G u Al a G u Phe G y Al a Leu Al a Val Al a Leu 90 Val Arg Pro Leu Pro Leu Page 77 Ar g Ser Asp Leu Al a Asp Al a Ser Met Trp 130 G n Gu 145 Gu lie G y Al a Ala I e Sy Asn 210 G u Val 225 Al a Asp Ser G u IVet Asn Ser His 290 Val G y 305 G u G n Ser Val Asp y Ser Al a Val Gene Sequence 24-2-06.txt Pro lie Asp u Asn Leu u Ile Ala Leu Gy Asn Sy 195 Val Leu Asp Lys Val 275 et Asn Ser Sy Al a Val 165 Tyr Sy Sy G u Leu 245 Lys Thr G u Lys Ser 325 Thr Lys 150 Gy Thr G u Val Sy 230 Pr o SIle Asp Asn Al a 310 G u Thr 135 Al a Gy Ser Lys Tyr 215 G n Phe G u Thr Tyr 295 Tyr Ar g Ser Al a Gu G u 185 Ar g Pr o Val Phe Al a 265 Tyr Sy Pr o SIle Al a Asp 170 Asp Tyr Sy Al a Val 250 Leu Al a Val Ar g G u 330 Asn 155 Gy Phe Leu Asn Ar g 235 Phe Thr Phe Leu Ser 315 Ser SIle Gu Lys Al a 205 Lys Lys Sy Sy Ar g 285 I le 3et G n Leu Al a Thr 190 Al a Leu Leu Sy Val 270 Pr o Asp Lys Asp G u Lys 175 I e Thr Ar g Sy Ser 255 I e I e Gy Lys Leu 335 <210> 61 <211> 900 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(877) <223> RXA02258 <400> 61 cgttgcaatt ctcgctcagt aaatccgaca cggccctttg ttagaaaaca aaacataaag ggccaccggg aaactttttt aagaaaggtg tgtttcacac atg gca cgt aag cca IVet Ala Arg Lys Pro 1 ctt atc gct ggt aac tgg aag atg aac ctg gat cac cag cag gca atc Leu lie Al a G y Asn Trp Lys IVet Asn Leu Asp His n G n Ala lie 15 ggc act gtt cag aag ctt gca ttc gcc ctt cca aag gaa tac ttc gag Sy Thr Val G n Lys Leu Ala Phe Ala Leu Pro Lys G u Tyr Phe G u 30 Page 78 Gene Sequence 24-2-06.txt aag gtt gac Lys Val Asp cag act ct c G n Thr Leu gac gt c t cc Asp Val Ser agc atg ct g Ser IVet Leu gag cgc cgc G u Arg Arg gca aag gca Al a Lys Al a 120 gag cca ct g G u Pro Leu 135 gag cag acc G u G n Thr 150 aac acc gtt Asn Thr Val gtt gct t cc Val Al a Ser ct g at c gt g Leu Ile Val 200 ctt tac ggt Leu Tyr G y 215 cag cct gac Sn Pro Asp 230 gaa gca ttc G u Al a Phe gtt gca gtc acc gtt Val Ala Val Thr Val cct ttc act Pro Phe Thr gac atc cgc tcc gtc Asp lie Arg Ser Val gag G u cac Hi s aag Lys t ac Tyr ct g Leu at c SIle aag Lys gcg Al a 170 gct Al a ctt Leu tct Ser gac Asp aag Lys 250 ggc Gy gag G u 75 tt g Leu cac Hi s t cc Ser cgt Ar g t cc Ser 155 t at Tyr gac Asp gca Al a gtt Val ggc G y 235 ct g Leu gac Asp 60 t cc Ser aac Asn aac Asn aac Asn gaa Gu 140 ctt Leu gag G u gct Al a ggc Sy aag Lys 220 gga G y gct Al a aag Lys ggt G y tgc Cys gag G u ggc G y 125 gct Al a gct Al a cca Pr o cag G n gac Asp 205 gca Al a ctt Leu ctt Leu gcg Al a tct Ser tct Ser 110 at c SIle ggc Gy ggc Sy gt g Val gaa G u 190 gag G u gaa G u gt c Val gt c Val acc Thr 80 gtt Val gag G u ccg Pr o cac Hi s gat Asp 160 gct Al a tgc Cys gct Al a gt c Val ggc G0y 240 act Thr ggt G y gt c Val ttg Leu at c SIle gtt Val 145 gct Al a at c l Ie aag Lys gag G u gct Al a 225 gct Al a ttc Phe gaa G u gtt Val gtt Val gt c Val 130 gag Gu gct Al a ggc Sy gct Al a ggc Sy 210 gag G u t cc Ser ggt Gy gtt Val gga G y gct Al a 115 tgc Cys t ac Tyr gag G u acc Thr at c l Ie 195 ct g Leu at c SIle ct c Leu gct Al a tct Ser cac Hi s 100 gcg Al a gtt Val gt c Val ct g Leu ggt Sy 180 cgc Ar g cgt Ar g gt c Val gac Asp cag G n gca Al a t cc Ser aag Lys ggt G y gtc Val gcc Al a 165 aag Lys ggt Sy att SIe ggt Sy ggt 245 gcc aac gct Al a Asn Al a 255 gcg agc gtt gct Ala Ser Val Ala taaagtacag agctttaaag cac <210> 62 <211> 259 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 62 IVet Ala Arg Lys Pro Leu lie Ala y Asn Trp Lys IVet Asn Leu Asp 1 5 10 Page 79 His n Gn Ala lIe y Thr Gene Sequence 24-2-06.txt Val G n Lys Leu Ala Phe Ala Leu Pro 25 Lys Asp Thr Gy Val Leu SIle Val 145 Al a SIle Lys G u Al a 225 Al a Ser Tyr Phe G u Arg Ser Val G y Al a G n Val Ser Al a G y Hi s Ser 100 Al a Al a Lys 115 Cys Val G y Tyr Val Val G u Leu Al a 165 Thr G y Lys 180 lie Arg y 195 Leu Arg Il e I e Val y Leu Asp G y 245 Al a Val Thr Val let Ar g Lys Pr o 135 G n Thr Al a SIle Tyr 215 Pr o Val Val G n Al a G u 105 Al a G u Ar g SIle Al a 185 G u Gy Val Al a G u Hi s Lys 90 Tyr Leu SIle Lys Al a 170 Al a Leu Ser Asp Lys 250 Val Gy G u Leu Hi s Ser Ar g Ser 155 Tyr Asp Al a Val Gy 235 Thr Asp Ser Asn Asn Asn G u 140 Leu G u Al a Gy Lys 220 Gy Val Lys Gy Cys G u Gy 125 Al a Al a Pr o G n Asp 205 Al a Leu Phe G u Tyr Tr p Asp Ser Thr Leu Tr p 175 Val Val Thr Gy G u Ala Phe Ala Leu Al a Al a Asn Al a Al a 255 <210> 63 <211> 1563 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1540) <223> RXN01225 <400> 63 tttgggctaa tgttgggggg agtgctttca actatccacg agagctgccc agtgataaac cccgggttaa ccccacgcct aagtcagtga aggacttttt atg acg cac aac cac 115 let Thr His Asn His 1 aag gac tgg aac gat cgc att gca gtt gcg gag gaa atg gtg ccg ttg 163 Lys Asp Trp Asn Asp Arg Ile Ala Val Ala G u G u IVet Val Pro Leu Page Gene Sequence 24-2-06.txt at c SIle cgt Ar g t ac Tyr gat Asp gac Asp ct g Leu acc Thr ggt Gy ggt Gy 150 ct g Leu ttc Phe aac Asn gat Asp ggc Gy 230 ggc Gy aac Asn cgc Ar g ctt Leu cgc Ar g ttg Leu gga Gy gcc Al a at c SIle 120 ct g Leu cgt Ar g aag Lys ggc Gy acc Thr 200 acc Thr tgg Tr p gcc Al a gt g Val cac Hi s aat Asn at c SIle gaa G u ct g Leu ct g Leu cca Pr o gcc Al a cgc Ar g gca Al a 170 at c SIle at c SIle t ac Tyr gac Asp gtt Val 250 ggc Gy cgc Ar g gt c Val at a SIle ct g Leu 75 gcc Al a gag G u act Thr cgc Ar g gcc Al a 155 t cc Ser acc Thr aag Lys ggc Gy cgc Ar g 235 gt a Val at c SIle aac Asn t ca Ser t cc Ser gt a Val t ac Tyr gac Asp gat Asp at c SIle 140 at c l Ie ttg Leu acc Thr gt c Val at c SIle 220 gaa G u ct c Leu aac Asn aac Asn at c SIle gaa G u at g tet ttc Phe ct c Leu 110 gt g Val gtt Val gt c Val cgt Ar g t at Tyr 190 t ac Tyr gac Asp ct g Leu gcg Al a acc Thr 270 gt g Val gat Asp ctt Leu aac Asn gaa G u 95 gcg Al a ct g Leu t cc Ser cgc Ar g cgt Ar g 175 gac Asp t cc Ser gcc Al a t cc Ser ccg Pr o 255 gac Asp gt g Val at c SIle cca Pr o ctt Leu gaa G u ccg Pr o t ac Tyr cgc Ar g aaa Lys 160 gat Asp aac Asn aat Asn gt c Val cag G n 240 ggc Gy at c SIle gcc Al a t cc Ser aag Lys gaa G u acc Thr gaa G u at t SIle ttc Phe 130 gca Al a ggt Gy gt c Val at c SIle cca Pr o 210 gt a Val ct c Leu ggc Gy gca Al a gt a Val tct Ser agc Ser gca Al a agc Ser ggt Gy 115 ggc Gy ct g Leu gaa Gu cac Hi s at c SIle 195 gcc Al a gac Asp aag Lys gat Asp gat Asp 275 ttc Phe cac Hi s t cc Ser tcg Ser acc Thr 100 gcg Al a cgc Ar g t at Tyr gaa Gu ggt Gy 180 tgg Tr p acc Thr aac Asn t cc Ser ctg Leu 260 gat Asp atc gtt tcc gca gca tca tgc Ile Val Ser Ala Ala Ser Cys acc acc aat Thr Thr Asn att acc cca II e Thr Pro gtg ct c Val Leu Page 81 Gene Sequence 24-2-06.txt aag Lys gtt Val 310 ggt Gy acc Thr aag Lys gct Al a aac Asn 390 at c SIle acc Thr cgc Ar g aac Asn 280 at c SIle t cc Ser cgc Ar g gct Al a acc Thr 360 ct c Leu ttc Phe tgg Tr p cac Hi s ct g Leu 440 gt c Val gat Asp acc Thr ggt Gy 330 aag Lys aat Asn ttg Leu cgc Ar g cgt Ar g 410 ggc Gy ctt Leu cgc Ar g cgc Ar g aat Asn 315 cgc Ar g gct Al a gcc Al a acc Thr cgt Ar g 395 t cc Ser at c SIle tac Ty r at c SIle t ac Tyr 300 gac Asp gca Al a gt a Val at c l Ie ct g Leu 380 gt g Val cca Pr o gtt Val gt g Val gt c Val 460 gt g Val aac Asn ggt Gy aag Lys 350 gtt Val acg Thr ct g Leu gtt Val ggt Gy 430 t ac Tyr gag G u gaa G u ct g Leu ct g Leu 335 gcg Al a cct Pr o gag G u cac Hi s gtt Val 415 ct a Leu gac Asp at c SIle ttc Phe at c SIle 320 aat Asn ctt Leu acc Thr gt g Val tct Ser 400 t cc Ser gcc Al a aac Asn gcc Al a ggc Gy 305 gac Asp at g Met cca Pr o cct Pr o gac Asp 385 gac Asp act Thr acc Thr gag G u ggc G5y 465 290 cac Hi s aac Asn gtt Val gag G u gac Asp 370 cgc Ar g ttg Leu gac Asp at c SIle ttc Phe 450 gt g Val gt a Val ttc Phe ct c Leu ct g Leu 355 gt g Val gat Asp cgc Ar g ttc Phe gca Al a 435 ggc Gy cgt Ar g gaa G u cac Hi s acc Thr 340 gaa G u t cc Ser gag G u cag G n gt g Val 420 acc Thr t ac Tyr cct Pr o 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 gtg tac ccg gag cgc agg cag cca gcc gta cta taggttatcc aagcctaata 1560 Val Tyr Pro u Arg Arg n Pro Ala Val Leu 470 475 480 cac 1563 <210> 64 <211> 480 <212> PRT <213> Corynebacter i um gl utam cum <400> 64 IVet Thr His Asn His Lys Asp Trp Asn Asp Arg Ile Ala Val Ala u 1 5 10 G u IVet Val Pro Leu IIe y Arg Leu His Arg Asn Asn Asn Val Val 25 Val Ser Val Phe y Arg Leu Leu Val Asn Val Ser Asp lie Asp lie 40 Page 82 Ilie Lys Ser His Arg Tyr ene Sequence 24- 2- 06. t xt Ala Arg His Ilie Ilie Ser LysC 55 3 u Leu Pr o Le u G31y Ser ValI G31y (31u 145 As n Ser As n As p ValI 225 Hi s Ly s Thr I Ie G31y 305 As p vb t Pr o Pr o As p 385 Ser A a Ser G31y 115 G31y Le u (31u Hi s I Ie 1 95 A a As p Lys5 As p As p 275 Pr o ValI Ph e Le u Le u 355 ValI As p Le u I Ie As p (31u I Ie As p As p 1 65 G31y A a I Ie Thr Lys5 245 Lys5 O31n Le u Thr Lys5 325 (31u G31y vb t ValI (31n 405 As p 70 As p Le u Thr G31y G31y 150 Le u Ph e As n As p G31y 230 G31y As n I Ie Lys5 ValI 310 G31y Thr Ly s A a As n 390 I Ie Le u Ar g As p Ar g 135 A a ValI As p G31y Tyr 215 Ar g ValI I Ie ValI ValI 295 Hi s Ser G31y Le u ValI 375 (31u Le u G31y A a I Ie 120 Le u Ar g Ly s G31y Thr 200 Thr Tr p A a ValI Ser 280 li1e Ser Ar g A a Thr 360 Le u Ph e Ar g O31n Ph e 1 05 As n Le u Le u Ar g Thr 1 85 Pr a O31u Ar g Ly s Tyr 265 A a As n Ph e Ar g A a 345 G31y As n Le u O31u Le u 90 Le u Pr a A a Ar g A a 1 70 le le Tyr As p ValI 250 G31y A a As p Thr G31y 330 Ly s As n Le u Ar g Le u A a O31u Thr Ar g A a 1 Ser Thr Ly s G31y Ar g 235 ValI I Ie Ser Ar g As n 315 Ar g A a A a Thr Ar g 395 As p Ser A a I Ie 125 Le u ValI Le u As p I Ie 205 As n G31y Thr Hi s Thr 285 G31y O31n A a Ser Ar g 365 As n Ser vb t Ph e Le u 110 ValI ValI ValI Ar g Tyr 1 Tyr As p Le u A a Thr 270 Thr ValI As n G31y Ly s 350 ValI Thr Le u As n (31u A a Le u Ser Ar g Ar g 1 As p Ser A a Ser Pr a 255 As p As n (31u Le u Le u 335 A a Pr a (31u Hi s Asp Leu Ar g O31n I Ie Asp Tr p I Ie Org Ser Pro Ou Val Val Ser 10 415 Page 83 Gene Sequence 24-2-06.txt Thr Asp Phe Val G y Thr Thr His Ala y I I e Val Asp G y Leu Ala 420 425 430 Thr lie Ala Thr G y Arg His Leu Val Leu Tyr Val Trp Tyr Asp Asn 435 440 445 Su Phe y Tyr Ser Asn n Val Ile Arg Ile Val Gu Gu Ile Ala 450 455 460 Sy Val Arg Pro Arg Val Tyr Pro G u Arg Arg G n Pro Ala Val Leu 465 470 475 480 <210> <211> 1563 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1540) <223> FRXA01225 <400> tttgggctaa tgttgggggg agtgctttca actatccacg cccgggttaa ccccacgcct aagtcagtga aggacttttt aag Lys at c SIle cgt Ar g t ac Tyr gat Asp gac Asp ct g Leu acc Thr tgg Tr p cgc Ar g ctt Leu cgc Ar g ttg Leu gga Gy gcc Al a at c SIle 120 gat Asp cac Hi s aat Asn at c SIle gaa G u ct g Leu ct g Leu cca Pr o at t SIle aac Asn t ca Ser t cc Ser gt a Val t ac Tyr gac Asp gat Asp gca Al a aac Asn gac Asp 45 aag Lys gat Asp agc Ser gct Al a at c SIle 125 gtt Val aac Asn at c SIle gaa G u at g tet ttc Phe ct c Leu 110 gt g Val gcg Al a 15 gt g Val gat Asp ctt Leu aac Asn gaa G u 95 gcg Al a ct g Leu gag G u gt g Val at c SIle cca Pr o ctt Leu 80 gaa G u ccg Pr o t ac Tyr agagctgccc agtgataaac atg acg cac aac cac 11! Met Thr His Asn His 1 gaa atg gtg ccg ttg 16 G u IVet Val Pro Leu gtt tcc gta ttc ggt 21 Val Ser Val Phe G y atc aag tct cac cgc Ile Lys Ser His Arg ctg gaa agc tcc ttg Leu G u Ser Ser Leu ggt acc gca tcg atc Sy Thr Ala Ser lie tcc gaa agc acc gac Ser G u Ser Thr Asp 100 gtc att ggt gcg gaa Val Ile Gy Ala u 115 ggt ttc ggc cgc atc 49! Sy Phe y Arg lie 130 ggt cgc ctg ctg gcc cgc atc ctg gtt tcc cgc gag gca ctg tat gac Gy Arg Leu Leu Ala Arg lie Leu Val Ser Arg u Ala Leu Tyr Asp Page 84 Gene Sequence 24-2-06.txt ggt Gy 150 ct g Leu ttc Phe aac Asn gat Asp ggc Gy 230 ggc Gy aac Asn at c SIle aag Lys gtt Val 310 ggt Gy acc Thr aag Lys gct Al a aac Asn 390 cgt Ar g aag Lys ggc Gy acc Thr 200 acc Thr tgg Tr p gcc Al a gt g Val t cc Ser 280 at c SIle t cc Ser cgc Ar g gct Al a acc Thr 360 ct c Leu ttc Phe cgc Ar g gca Al a 170 at c SIle at c SIle t ac Tyr gac Asp gtt Val 250 ggc Gy gca Al a gat Asp acc Thr ggt Gy 330 aag Lys aat Asn ttg Leu cgc Ar g gcc Al a 155 t cc Ser acc Thr aag Lys ggc Gy cgc Ar g 235 gt a Val at c SIle t ca Ser cgc Ar g aat Asn 315 cgc Ar g gct Al a gcc Al a acc Thr cgt Ar g 395 at c l Ie ttg Leu acc Thr gt c Val at c SIle 220 gaa G u ct c Leu aac Asn tgc Cys t ac Tyr 300 gac Asp gca Al a gt a Val at c l Ie ct g Leu 380 gt g Val gt g Val ct g Leu gat Asp at c SIle 205 aat Asn ggc Gy acc Thr cac Hi s acc Thr 285 ggc Gy cag G n gca Al a t cc Ser cgc Ar g 365 aac Asn t cc Ser gt c Val cgt Ar g t at Tyr 190 t ac Tyr gac Asp ct g Leu gcg Al a acc Thr 270 acc Thr gt g Val aac Asn ggt Gy aag Lys 350 gtt Val acg Thr ct g Leu cgc Ar g cgt Ar g 175 gac Asp t cc Ser gcc Al a t cc Ser ccg Pr o 255 gac Asp aat Asn gaa Gu ct g Leu ct g Leu 335 gcg Al a cct Pr o gag G u cac Hi s aaa Lys 160 gat Asp aac Asn aat Asn gt c Val cag G n 240 ggc Gy at c SIle gcc Al a ttc Phe at c SIle 320 aat Asn ctt Leu acc Thr gt g Val tct Ser 400 ggt Gy gt c Val at c SIle cca Pr o 210 gt a Val ct c Leu ggc Gy gca Al a acc Thr 290 cac Hi s aac Asn gtt Val gag G u gac Asp 370 cgc Ar g ttg Leu gaa G u cac Hi s at c SIle 195 gcc Al a gac Asp aag Lys gat Asp gat Asp 275 cca Pr o gt a Val ttc Phe ct c Leu ct g Leu 355 gt g Val gat Asp cgc Ar g gaa G u ggt Gy 180 tgg Tr p acc Thr aac Asn t cc Ser ct g Leu 260 gat Asp gt g Val gaa G u cac Hi s acc Thr 340 gaa G u t cc Ser gag G u cag G n 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 atc gac tgg atc cgt tcc cca gag gtt gtt tcc act gac ttc gtg ggc Ile Asp Trp lie Arg Ser Pro u Val Val Ser Thr Asp Phe Val Gy Page 410 acc acc cac gcg ggc atc gtt gat g Thr Thr His Ala y I I e Val Asp C 425 4 cgc cac ctg gtg ctt tac gtg tgg t Arg His Leu Val Leu Tyr Val Trp T 440 445 aac cag gtc att cgc atc gtc gag g Asn n Val Ile Arg Ile Val Gu C 455 460 gtg tac ccg gag cgc agg cag cca g Val Tyr Pro G u Arg Arg G n Pro A 470 475 cac <210> 66 <211> 480 <212> PRT <213> Cor ynebact eri um gl ut am cum Sequence 24-2-06. t xt 415 420 Igt cta gcc acc atc gca acc ggt 1411 3y Leu Ala Thr lie Ala Thr y .30 435 ac gac aac gag ttc ggc tac tcc 1459 yr Asp Asn G u Phe G y Tyr Ser 450 lag atc gcc ggc gtg cgt cct cgc 1507 3u lie Ala y Val Arg Pro Arg 465 cc gta cta taggttatcc aagcctaata 1560 1l a Val Leu 480 1563 <400> 66 IVet Thr His Asn His Lys Asp Trp Asn 1 G u Val SIle Leu Gy Ser Val Gy G u 145 Asn Ser Asn Val Val Ser Ser Al a Ser Gy 115 Gy Leu G u Hi s SIle 195 5 Leu Gy Ar g Leu l Ie Asp G u SIle Asp Asp 165 Gy Al a Gy Leu Al a I le Leu Ar g Asp Ar g 135 Al a Val Asp Gy Tyr 215 Leu Val Hi s Ar g G n Phe 105 Asn Leu Leu Ar g Thr 185 Pr o Asp 10 Hi s Asn SIle G u Leu 90 Leu Pr o Al a Ar g Al a 170 I e I e Arg Ile Ala Val Ala u Ar g Val SIle Leu Al a G u Thr Ar g Al a 155 Ser Thr Lys Asn Asp Lys Asp Ser Al a SIle 125 Leu Val Leu Asp SIle 205 Val Asp Leu Asn G u Al a Leu Ser Ar g Ar g 175 Asp Ser Asp Pro Ala Thr Ile Asp 210 Thr u Tyr y Ile Asn Asp Ala Val 220 Page 86 Gene Sequence 24-2-06.txt Val 225 Hi s Lys Thr SIle Gy 305 Asp let Pr o Pr o Asp 385 Asp Thr Thr G u Gy 465 Asp Lys Asp Asp 275 Pr o Val Phe Leu Leu 355 Val Asp Ar g Phe Al a 435 Gy Thr Lys 245 Lys G n Leu Thr Lys 325 G u Gy let Val G n 405 Gy Gy Ser Arg Trp Arg Asp Val SIle Val Val 295 Hi s Ser Gy Leu Val 375 G u Asp Thr Hi s G n 455 Al a Val Ser 280 I le Ser Ar g Al a Thr 360 Leu Phe Tr p Hi s Leu 440 Val Lys Tyr 265 Al a Asn Phe Ar g Al a 345 Gy Asn Leu SIle Al a 425 Val SIle Ar g 235 Val SIle Ser Ar g Asn 315 Ar g Al a Al a Thr Ar g 395 Ser l Ie Tyr SIle Ar g 475 0 u 0 y Leu Ser 0 n Leu Asn Cys Tyr 300 Asp Al a Val l Ie Leu 380 Val Pr o Val Val Val 460 Thr Hi s Thr 285 Gy G n Al a Ser Ar g 365 Asn Ser G u Asp Tr p 445 G u Al a Thr 270 Thr Val Asn Gy Lys 350 Val Thr Leu Val Gy 430 Tyr G u Pr o 255 Asp Asn G u Leu Leu 335 Al a Pr o G u Hi s Val 415 Leu Asp SIe Val Arg Pro Arg Val Tyr Pro u Arg 470 Sn Pro Ala Val <210> 67 <211> 1125 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1102) <223> RXA02256 <400> 67 ttgggttctg tcagctcaag aattcttgag tgaccgatgc tctgattgac ctaactgctt gacacattgc atttcctaca atctttagag gagacacaac atg acc att cgt gtt 115 IVet Thr Ile Arg Val Page 87 Gene Sequence 24- 2- 06. txt 1 ggt att aac gga ttt ggc cgt atc gga cgt aac ttc ttc cgc gca gtt 163 Gy I e Asn y Phe y Arg lie y Arg Asn Phe Phe Arg Ala Val 15 ctg gag cgc agc gac gat ctc gag gta gtt gca gtc aac gac ctc acc 211 Leu G u Arg Ser Asp Asp Leu G u Val Val Ala Val Asn Asp Leu Thr 30 gac aac aag acc ctt tcc acc ctt ctc aag ttc gac tcc atc atg ggc 259 Asp Asn Lys Thr Leu Ser Thr Leu Leu Lys Phe Asp Ser Ile IVet G y 45 cgc ctt ggc cag gaa gtt gaa tac gac gat gac tcc atc acc gtt ggt 307 Arg Leu Gy n G u Val G u Tyr Asp Asp Asp Ser lie Thr Val y 60 ggc aag cgc atc gct gtt tac gca gag cgc gat cca aag aac ctg gac 355 Sy Lys Arg lie Ala Val Tyr Ala G u Arg Asp Pro Lys Asn Leu Asp 75 80 tgg gct gca cac aac gtt gac atc gtg atc gag tcc acc ggc ttc ttc 403 Trp Ala Ala His Asn Val Asp Ile Val Ile Gu Ser Thr y Phe Phe 95 100 acc gat gca aac gcg gct aag gct cac atc gaa gca ggt gcc aag aag 451 Thr Asp Al a Asn Al a Al a Lys Al a Hi s IIe G u Al a G y Al a Lys Lys 105 110 115 gtc atc atc tcc gca cca gca agc aac gaa gac gca acc ttc gtt tac 499 Val Ile Ile Ser Ala Pro Ala Ser Asn G u Asp Ala Thr Phe Val Tyr 120 125 130 ggt gtg aac cac gag tcc tac gat cct gag aac cac aac gtg atc tcc 547 G y Val Asn His G u Ser Tyr Asp Pro G u Asn His Asn Val lie Ser 135 140 145 ggc gca tct tgc acc acc aac tgc ctc gca cca atg gca aag gtc cta 595 Gy Ala Ser Cys Thr Thr Asn Cys Leu Ala Pro IVet Ala Lys Val Leu 150 155 160 165 aac gac aag ttc ggc atc gag aac ggc ctc atg acc acc gtt cac gca 643 Asn Asp Lys Phe Gy Ile G u Asn y Leu IVet Thr Thr Val His Ala 170 175 180 tac act ggc gac cag cgc ctg cac gat gca cct cac cgc gac ctg cgt 691 Tyr Thr G y Asp G n Arg Leu His Asp Ala Pro His Arg Asp Leu Arg 185 190 195 cgt gca cgt gca gca gca gtc aac atc gtt cct acc tcc acc ggt gca 739 Arg Ala Arg Ala Ala Ala Val Asn Ile Val Pro Thr Ser Thr Gy Ala 200 205 210 gct aag gct gtt gct ctg gtt ctc cca gag ctc aag ggc aag ctt gac 787 Ala Lys Ala Val Ala Leu Val Leu Pro G u Leu Lys y Lys Leu Asp 215 220 225 ggc tac gca ctt cgc gtt cca gtt atc acc ggt tcc gca acc gac ctg 835 G y Tyr Ala Leu Arg Val Pro Val lie Thr G y Ser Ala Thr Asp Leu 230 235 240 245 acc ttc aac acc aag tct gag gtc acc gtt gag tcc atc aac gct gca 883 Thr Phe Asn Thr Lys Ser G u Val Thr Val u Ser lie Asn Ala Ala 250 255 260 atc aag gaa gct gca gtc ggc gag ttc ggc gag acc ctg gct tac tcc 931 Ile Lys Gu Ala Ala Val Gy u Phe Gy u Thr Leu Ala Tyr Ser Page 88 265 2 gaa gag cca ctg gtt tcc acc gac a G u G u Pro Leu Val Ser Thr Asp I 280 285 atc ttc gac gct ggc ctg acc aag g Ile Phe Asp Ala Gy Leu Thr Lys 295 300 gtt tcc tgg tac gac aac gag tgg g Val Ser Trp Tyr Asp Asn G u Trp C 310 315 ctg acc gag ctc gta gct tcc aag c Leu Thr G u Leu Val Al a Ser Lys L 330 cgt <210> 68 <211> 334 <212> PRT <213> Cor ynebact eri um gl ut ai cum Sequence 24-2-06. t xt 70 275 t c gtc cac gat tcc cac ggc tcc le Val His Asp Ser His G y Ser 290 itc tcc ggc aac acc gtc aag gtt (al Ser G y Asn Thr Val Lys Val 305 igc tac acc tgc cag ctc ctg cgt 3y Tyr Thr Cys G n Leu Leu Arg 320 325 t c taattagttc acatcgctaa .eu 979 1027 1075 1122 1125 <400> 68 Met Thr li e Arg Val G y I e Asn G y 1 Phe Val Asp Ser Pr o Ser Al a Al a Hi s 145 Met Thr Hi s Ar g Asp I le Thr Asn Gy Al a 115 Phe Val Lys Val Asp 195 5 Val Thr Gy Gy Asp Phe Lys Tyr Ser Leu 165 Al a Ar g G u Asn Leu Lys Al a Asp SIle Val 135 Al a Asp Thr Al a Lys 215 Ar g Lys 40 Gy Ar g Al a Al a SIle 120 Asn Ser Lys Gy Ar g 200 Ser Thr G n SIle Hi s Asn 105 Ser Hi s Cys Phe Asp 185 Al a Phe 10 Asp Leu G u Al a Asn 90 Al a Al a G u Thr Gy 170 G n Al a Gy Asp Ser Val Val 75 Val Al a Pr o Ser Thr 155 I le Ar g Al a SIle G u Leu Tyr Al a SIle Al a Ser 125 Asp Cys Asn Hi s Asn 205 Ar g Val Lys Asp Ar g SIle l e G u G u Al a Leu 175 Al a Val Asn Al a Phe Asp Asp G u G u Asp Asn Pr o 160 Met Pr o Pr o Thr Ser Thr y Al a Al a 210 Al a Val Al a Leu Val Leu Pro G u Leu 220 Page 89 Ly s 225 Ser Ser Thr As p As n 305 Cys Ly s Thr As n Al a 275 Hi s ValI Le u As p Le u 245 Al a Ser Ser ValI Ar g 325 Tyr Ph e Ly s G31u Ph e 295 Ser Thr ene Sequence 24- 2- 06. txt Ala Leu Arg Val Pro Val lie 235 Asn Thr Lys Ser G3 u ValI Thr 250 (31u Al a Al a Val (31y G31u Phe 265 270 Pr o Leu Val Ser Thr Asp lie 280 285 Asp Al a G31y Leu Thr Lys Val 300 Tr p Tyr Asp Asn G3 u Tr p G3 y 315 (31u Leu Val Al a Ser Lys Leu 330 Thr ValI 255 G31y ValI Ser Tyr <210O> 69 <21 1> 1338 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 ODS <222> (1315) <223> RXA02257 <400> 69 at accggt gc cagcgccaca caat gt gtgg caatct ggga gaagaat t tt t taaacaat c aaat ct ccaa ggagt acggc cagt gcat ca cat tgcacca ct c Le u cga Ar g aag Ly s ggt G31y gag G31u g at As p g ac As p g ac As p g ac As p cga Ar g gca Al a aac As n ct t Le u c ac Hi s ct c Le u aat As n at t I Ie gt c ValI aag Ly s c ag (31n cgc Ar g gac As p gt t ValI gcc Al a at c SI e t ac Tyr 75 t ac Tyr gca Al a g aa G31u ccc Pr o t cc Ser gt c ValI t cc Ser gt t ValI aac As n ggc G31y ct c Le u ct a Le u 45 at g vb t ct c Le u gca Al a ggc G31y gt a ValI aac As n cca Pr o gct Al a gca Al a ct t Le u ct g Le u 110 g ac As p 15 g at As p ac c Thr c ac Hi s cct Pr o gcc Al a 95 ac c Thr gga G31y g ac As p ct t Le u ct t Le u gt c ValI 80 gca Al a gag (31u at g gct gt t [Vb t Al a Val 1 cgc cac gt c Ar g Hi s Val cgc gag at c Ar g 31u Ilie aaa gca ctg Lys Al a Leu ggc cgc cca G31y Ar g Pr o gct gag gca Al a 03 u Al a gac gtt gtt Asp Val Val ggc gac at c 03 y Asp Il e 115 aag acc Lys Thr at c gt t I e Val acc gat Thr As p agc gaa Ser 03 u aag ggc Lys 03 y ct c t cc Leu Ser ggc gaa (31y (1u 1 00 ct g ct c Leu Leu Page Gene Sequence 24-2-06.txt ct g Leu gag G u ggc Sy 150 t cc Ser gt a Val gca Al a ggt Gy ggc Gy 230 cag G n gca Al a t cc Ser at c SIle aac Asn 310 cca Pr o at c SIle gtt Val aac Asn aac Asn 120 acc Thr ttc Phe t ac Tyr acc Thr t ac Tyr 200 at t SIle at g let ct c Leu ttc Phe ttt Phe 280 gaa G u ggt Gy ggc Gy cag G n ggc Sy 360 gac Asp gtg cgc ttc gac cca cgc gaa Val Arg Phe Asp Pro Arg u acc tcc aag Thr Ser Lys 130 gac gag gca Asp G u Ala ttc Phe tct Ser at t SIle 170 at t SIle gt g Val gcg Al a t ac Tyr cag G n 250 gac Asp aag Lys tgg Tr p gtt Val ttc Phe 330 at c SIle ggc Gy ttc Phe gct Al a gac Asp 155 gca Al a t cc Ser gtt Val ct g Leu acc Thr 235 gaa G u aag Lys gac Asp at g bet ct c Leu 315 gag G u at c SIle gac Asp t cc Ser cag G n 140 ggc Gy aag Lys gtt Val ct c Leu gct Al a 220 ttc Phe gaa G u at c SIle gca Al a tct Ser 300 agc Ser ttc Phe gat Asp t cc Ser cac Hi s 380 125 gag G u ttc Phe ttg Leu ct g Leu ggt Gy 205 gcc Al a ct c Leu at g let gtt Val gag G u 285 ctt Leu acc Thr gca Al a gca Al a gca Al a 365 at c SIle ct c Leu ggt Gy ct g Leu gaa G u 190 gga Gy aag Lys gca Al a aag Lys ct c Leu 270 aag Lys gac Asp gct Al a gca Al a act Thr 350 gca Al a t cc Ser gca Al a gtt Val cca Pr o 175 aag Lys t cc Ser gct Al a gct Al a gct Al a 255 cca Pr o cag G n at c SIle aag Lys ttc Phe 335 gca Al a t cc Ser acc Thr gct Al a gt c Val 160 cac Hi s at c SIle aag Lys gac Asp cag G n 240 acc Thr gtt Val at c SIle gga Sy acc Thr 320 tct Ser ggc G y gtt Val ggt G y ctt Leu 145 cac Hi s t ac Tyr gca Al a gt c Val aag Lys 225 gga Sy tgc Cys gac Asp gtt Val cca Pr o 305 at c SIle gaa G u aac Asn cgc Ar g ggt 35y 385 gca Al a cgc Ar g gct Al a gaa G u tct Ser 210 at c SIle cac Hi s acc Thr ct g Leu gac Asp 290 gag G u ttc Phe ggc G y gac Asp gt g Val 370 ggc G y gca Al a gca Al a ggc G y t ca Ser 195 gac Asp at c SIle aac Asn gac Asp gtt Val 275 ct g Leu t cc Ser tgg Tr p acc Thr gca Al a 355 ct c Leu gca Al a gac Asp cag G n gga G y 180 cca Pr o aag Lys gt c Val gtt Val ct g Leu 260 gca Al a gac Asp gt c Val aac Asn cgc Ar g 340 ttc Phe ggc G y t cc Ser aac Asn acc Thr 165 ct g Leu gag G u at c lle ggt Gy cag G n 245 ct c Leu gca Al a t cc Ser aag Lys ggc G y 325 ggc G y t cc Ser ct g Leu ct c Leu 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 Page 91 Gene Sequence 24- 2- 06. txt gag tac ctt gaa ggc aag gaa ctc cca ggc gtt gca att ctc gct cag Ou Tyr Leu Ou Gy Lys u Leu Pro Gy Val Ala lle Leu Ala n 390 395 400 405 taaatccgac acggcccttt gtt <210> <211> 405 <212> PRT <213> Corynebacter i um gl ut ai cum 1315 1338 <400> [et Al a Val 1 Arg His Val Arg u lie Lys Ala Leu G y Arg Pro Ala G u Al a Asp Val Val G y Asp Ile 115 Ser Lys Asp 130 Leu Al a Al a 145 His Arg Ala Tyr Al a G y Al a G u Ser 195 Val Ser Asp 210 Lys Ile lle 225 G y Hi s Asn Cys Thr Asp Asp Leu Val 275 Lys Thr Leu Lys 5 Ile Val Arg Ser Thr Asp Lys G y Ser G u G y G y 55 Lys G y G u Val 70 Leu Ser Asp G u G y G u Asp Al a 100 Leu Leu Leu G u G u Ala G u Ar g 135 Asp Asn G y Al a 150 G n Thr Ser Val 165 Sy Leu Val G u 180 Pro G u Al a Pro Lys Ile G y Val 215 Val y y y 230 Val n G n Ser 245 Leu Leu Ala Arg 260 Ala Ala Ser G u Leu Phe 25 I le Lys G u Gy G u 105 Val Al a Val Asp G u 185 Val G u Cys Leu Gy 265 Asn Leu 10 Asn SIle Val Lys G n 90 Ar g Ar g Phe Ser SIle 170 I le Val Al a Tyr G n 250 Asp Lys Asp Val Al a SIle Tyr Tyr Al a Phe Al a Asp 155 Al a Ser Val Leu Thr 235 G u Lys Asp G u Pr o Ser Val Ser Val Asn Asp G n 140 Sy Lys Val Leu Al a 220 Phe Su I e Al a Sy Leu Leu bet Leu Al a Sy Pr o 125 G u Phe Leu Leu Sy 205 Al a Leu bet Val G u 285 Val Asn Pr o Al a Al a Leu Leu 110 Ar g Leu Sy Leu G u 190 Sy Lys Al a Lys Leu 270 Lys Asp Asp Thr Hi s Pr o Al a Thr G u Al a Val Pr o 175 Lys Ser Al a Al a Al a 255 Pr o G n Val Asp Leu Asp Ser Ile Pro u Oy Trp IVet Ser Leu Asp Ile y Page 92 Pr o 305 li1e G31u As n Ar g G31y 385 A a Ser Tr p Thr A a 355 Le u A a Le u Ly s G31y 325 G31y Ser Le u Le u O31n 405 295 Ph e A a ValI G31u 375 Ty r ene Sequence 24- 2- 06. txt 300 (31y G31u ValI Leu Ser Thr Ala 315 (31y Val Phe (31u Phe Ala Ala 330 (3 n A a Il e Il e Asp A a Thr 345 350 (3 y 03 y 03 y Asp Ser A a A a 360 365 Asp G31y Phe Ser Hi s Ilie Ser 380 Leu (31u (31y Lys G31u Leu Pr o 395 <210O> 71 <21 1> 1398 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1375) <223> RXA00235 <400> 71 cgaaacaaga t tcgt gcaac aat tgggt gt agacgt gat t at aat tct ag t tagct ccca agt tggcat a ggaggccaca gaagacat tt gat cacgt ga c ac Hi s gag G31u cca Pr o ggt G31y gt c ValI c ag O31n aag t tc Ph e gag O31u G31y g at As p g aa O31u ct c Le u cgc Ar g cgc Ar g t tc Ph e t cc Ser t ac Tyr at c I Ie g ac As p ggt (31y gaa O31u ct g Le u acc Thr ct g Le u ggc G31y 75 gaa O31u gca A a at t I Ie g at As p ggc G31y ggc G31y g ac As p gca A a aac As n ct c Le u g ac As p gt c ValI 45 aag Lys5 gag O31u at g vb t gca A a g ac As p ggt G31y c ac Hi s ggc G31y ct c Le u at c I Ie at c I Ie 110 t cc Ser 15 t cc Ser gag O31u gt t ValI gct A a aag Lys5 95 ct t Le u cgc Ar g c ac Hi s gct A a tt g Le u ggc G31y ct t Le u ggt (31y gt g ValI 1 ggt G31y ggt G31y cat Hi s aag Lys5 ct a Le u g at As p gt t ValI gct A a aac As n gt c ValI gag O31u gca A a gag O31u G31y t cc Ser g aa O31u cca Pr o gca A a ct g Le u gt t ValI gct A a ac c Thr at g 115 at c I Ie ac c Thr ggt G31y cgt Ar g g aa O31u g ac As p gcc A a 1 00 gct A a Page 93 Gene Sequence 24-2-06.txt gca Al a ggt Sy acc Thr 150 at c SIle gcg Al a t cc Ser acc Thr ttc Phe 230 gag Gu gca Al a at c SIle acc Thr gac Asp 310 aag Lys acc Thr t cc Ser gac Asp gct Al a 120 cca Pr o ggc G y cca Pr o gt c Val gga G y 200 gag Gu cca Pr o ttc Phe gag Gu t cc Ser 280 ct c Leu ttc Phe gcc Al a acc Thr at g Met 360 gca Al a gct Al a gca Al a cac Hi s ggt Sy 170 cac Hi s ggc G y ctt Leu aag Lys gac Asp 250 gca Al a gag Gu gca Al a acc Thr t cc Ser 330 gac Asp cac Hi s gca Al a gat Asp cac Hi s gct Al a 155 gca Al a gca Al a gat Asp gac Asp gac Asp 235 ggc Gy aac Asn gac Asp acc Thr aac Asn 315 at c SIle gct Al a cgt Ar g ct c Leu t cc gca ggc Ser Al a G y 125 gtt ctt cca Val Leu Pro 140 gac t cc ggt Asp Ser G y gag acc ttc G u Thr Phe ct g aag t cc Leu Lys Ser 190 gag ggc ggc Su G y y 205 ctt atc gtt Leu Ile Val 220 atc gct ct t I le Al a Leu acc tac cac Thr Tyr Hi s gtt tac gct Val Tyr Al a 270 cca ct g cag Pro Leu G n 285 at c ggc gac Ile G y Asp 300 cct gag cgc Pro G u Ar g ctg gtt aag Leu Val Lys gtc gac at g Val Asp Vbet 350 t cc ggt gag Ser G y G u 365 aac t gt ggc Asn Cys y 380 ctc cca ctg ttc Leu Pro Leu gtt Val gtt Val tct Ser 175 gt c Val ttc Phe gag G u gct Al a ttc Phe 255 gag G u gaa G u aag Lys ct g Leu gt g Val 335 gct Al a acc Thr cag G n cca Pr o gac Asp 160 gag G u at c SIle gct Al a gca Al a ct g Leu 240 gaa G u ct c Leu gat Asp gtt Val aag Lys 320 aac Asn cac Hi s gag G u at c SIle at g bet 145 gtt Val gct Al a aag Lys cct Pr o at c l Ie 225 gac Asp ggt G y gtt Val gac Asp cag G n 305 gag G u cag G n cgc Ar g gac Asp aag Lys 385 Phe 130 at g bet cag G n ct c Leu gaa G u t cc Ser 210 gag G u gtt Val ggc G y gac Asp tgg Tr p 290 at c SIle ggc G y at c SIle gca Al a acc Thr 370 act Thr cgc t ac at c Arg Tyr Il e aac Asn gaa G u cgc Ar g aag Lys 195 gt c Val aag Lys gct Al a cag G n gcg Al a 275 gag G u gtt Val at c SIle ggt G y ggc G y 355 acc Thr ggt Gy at c SIe ttc Phe aac Asn 180 ggc G y ggc G y gct Al a t cc Ser cac Hi s 260 t ac Tyr ggt G y ggc G y gct Al a acc Thr 340 t ac Tyr att SIe gct Al a 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 Page 94 gca cgt t cc Al a Ar g Ser 390 cag ctg ctt 03 n Leu Leu cgc ttt cag Ar g Phe O31n ene Sequence 24- 2- 06. txt gac cgt gtc gca aag tac aac cag ct t ct c cgc at c gag Asp Ar g Val Ala Lys Tyr Asn On Leu Leu Arg Ilie O31u 395 400 405 ggc gac gcc ggc gtc tac gca ggt cgc agc gca ttc cca O31y Asp Al a G31y ValI Ty r Al a G31y Ar g Ser Al a Ph e Pr o 410 415 420 ggc t aaat aaaag cgctt t tcga cgc G31y 425 1315 1363 1398 <210O> 72 <21 1> 425 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 72 Val Ala 31y As n O3 y Val Hi s 03 u Lys Al a Leu 03 u As p G31y Val Ser Leu Phe 130 Ivb t b t 145 Val O31n Al a Leu Lys 03 u Pr o Ser 210 le O31u 225 31u I I e I'b t Pr o Thr Val Al a 03 y Val Leu Arg Asp Val 31u Asn Al a Asp Asp Thr Al a Asn 1 00 I'b t Al a Val 115 Ar g Tyr Ilie Asn Il e Il e (3 u Phe [ib t 1 65 Ar g Asn G31y 1 80 Lys 03 y Leu 1 95 Val G31y Ser Lys Al a 03 y Hi s (31u Pr o G31y ValI 70 O31n Ly s Al a O31y Thr 150 li1e Al a Ser Thr Ph e 230 ValI Al a Ser O31y 55 As n Ar g Ser Ly s O31y 135 O31y Al a O31u Thr Ar g 215 Thr Ph e O31u O31y 40 As p O31u Le u Ar g Al a 120 Pr o O31y Pr o ValI O31y 200 O31u Pr o Al a ValI Al a Ar g O31u I Ie Le u 1 Al a As n Al a I Ie Tyr 1 Le u Al a (31y Ar g 10 Ph e Ser Tyr I Ie As p 90 O31y Al a Al a Hi s O31y 1 70 Hi s O31y Le u Ly s As p 250 O31u Le u Thr Le u O31y O31u Al a As p Hi s Al a 1 Al a Al a As p As p As p 235 I Ie As p O31y O31y As p Al a As n Ser ValI 140 As p O31u Le u O31u Le u 220 li1e Le u As p ValI Ly s O31u vb t Al a Al a 125 Le u Ser Thr Ly s O31y 205 li1e Al a Ser Ser O31u ValI Al a Ly s Le u Le u ValI ValI Ser 1 ValI Ph e O31u Al a Asp Val Al a Ser Ser 03 u Phe Phe Lys 245 Oy Thr Tyr His Phe Ou 255 Page Sy Val Asp G n 305 G u G n Ar g Asp Lys 385 Leu Ar g G n Al a 275 G u Val SIle Gy Gy 355 Thr Gy Ar g Al a Ser Pr o Tyr Asp Lys 325 Leu Thr Al a Pr o G u 405 Pr o Al a SIle Thr Asp 310 Lys Thr Ser Asp Al a 390 G n Ar g Al a Val Asn 295 Phe Al a G u et Leu 375 Ar g Leu Phe Gene Sequence 24-2-06.txt G u IVet Ala Asn Val Tyr Ala 265 270 Ser Ile u Asp Pro Leu n 280 285 Leu Thr Ala Thr Ile y Asp 300 Phe Val Thr Asn Pro G u Arg 315 Ala Asn Ser lie Leu Val Lys 330 Thr Phe Asp Ala Val Asp IVet 345 350 IVet Ser His Arg Ser G y G u 360 365 Ala Val Ala Leu Asn Cys y 380 Ser Asp Arg Val Ala Lys Tyr 395 Leu y Asp Ala y Val Tyr 410 4n y 425 G u G u Lys Leu Val 335 Al a Thr G n Asn Al a 415 <210> 73 <211> 1554 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1531) <223> RXA01093 <400> 73 cagaggcgta gaacat t gtc tgt t cacact ctgggtcgca gtagtacctg tggcttgagg gggaatgacg tactaggctt cga Ar g gga G y ttc Phe cgc Ar g ct c aag Lys ct g Leu cat Hi s gcg Al a gga gt a Val ttg Leu gac Asp gag agu aag tgt Cys gt a Val cac Hi s aag Lys at c acc Thr gaa G u cca Pr o act Thr cgt ct a Leu gac Asp gat Asp 45 ggc Gty ct t ggc G y ggc G y cat Hi s cgt Ar g ggc cca gcg Pro Al a 15 at g gat IVet Asp gag caa G u G n gca gt c Al a Val cgt ttc Page 96 agat t catcg agaat t aatg atg ggc gtg gat aga IVet y Val Asp Arg 1 gtg gct agt gca gat Val Ala Ser Ala Asp gtt gct cgc ctc aac Val Ala Arg Leu Asn aac tac aag tgg gtc Asn Tyr Lys Trp Val ggt att ctc gca gac G y I e Leu Al a Asp act gac ggc gca acc Leu Gn Gy Pro Lys Ile Gene Sequence 24-2-06. t xt Arg Leu Gy Arg Phe Thr Asp Gy Ala Thr gt g Val gga Gy gcg Al a gt c Val ggc Gy 150 at t SIle gcc Al a cca Pr o cgt Ar g ct c Leu 230 gac Asp cgc Ar g acc Thr gaa Gu at g bet 310 cgc Ar g gaa Gu cac Hi s cca Pr o 120 gtt Val cca Pr o gt a Val aag Lys gat Asp 200 cct Pr o cca Pr o ggc G y at c SIle at g Net 280 tct Ser tct Ser at g bet ggc G y cgt Ar g gac Asp gt c Val t cc Ser gca Al a 170 ggc G y gaa Gu at c SIle gt g Val gag G u 250 at t SIle gat Asp gt g Val gaa Gu cgc Ar g 330 gag G u gt g Val cgc Ar g gaa Gu aac Asn 155 ct g Leu gt g Val ct c Leu gcc Al a tt g Leu 235 gtt Val gcc Al a t cc Ser gca Al a act Thr 315 at t SIle acc Thr t cc Ser ct g Leu ggt Gy 140 aac Asn t cc Ser gac Asp gtt Val aag Lys 220 gca Al a cct Pr o cgt Ar g at g bet aac Asn 300 t ca Ser gtt Val cgg Ar g acc Thr 110 gtt Val gac Asp ggt Gy aag Lys at t SIle 190 aag Lys gaa Gu gac Asp gag G u aac Asn 270 gag Gu gt g Val ggc Gy ttc Phe at c I e 95 t ac Tyr gat Asp gt c Val gtt Val gat Asp 175 gca Al a at c SIle aag Lys gcc Al a gag G u 255 gca Al a aac Asn ct c Leu aaa Lys gct Al a 335 acc Thr aag Lys gac Asp at c SIle t cc Ser 160 at c SIle ct g Leu at g et cca Pr o gt c Val 240 gtt Val aag Lys t cc Ser gat Asp gat Asp 320 gaa Gu gtt Val aat Asn ggc Gy tgt Cys 145 ct g Leu cgt Ar g t cc Ser gac Asp gag G u 225 at g Net cca Pr o cca Pr o cgc Ar g ggc Gy 305 ccg Pr o acc Thr gac Asp ct g Leu aag Lys 130 gag Gu cca Pr o gac Asp ttc Phe gaa Gu 210 gct Al a gtt Val ct g Leu gtt Val cca Pr o 290 gca Al a cac Hi s gac Asp gat Asp gca Al a 115 gtt Val gtt Val ggt Gy ct g Leu gt a Val 195 gaa Gu gt c Val gct Al a gtt Val at c SIle 275 acc Thr gat Asp aac Asn ggt Gy gt a Val 100 aaa Lys ggc Gy gtt Val at g Net cgc Ar g 180 cgt Ar g ggt G y acc Thr cgt Ar g cag G n 260 gt g Val cgt Ar g gct Al a gtt Val cgc Ar g 340 gag G u gac Asp ct c Leu gag G u gat Asp 165 ttc Phe t cc Ser cgt Ar g t cc Ser ggt G y 245 aag Lys gca Al a gcg Al a gt c Val gt g Val 325 gt c Val 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 cca gac ctg acc cac atc cct cgc act aag cgt ggc gtt att tcc tac Page 97 Pro Asp Leu Thr His Ile Pro 345 Gene Sequence 24-2-06.txt Arg Thr Lys Arg Gy Val lie Ser Tyr 350 355 tct gca cgt gat atc gcc gag cgc ctc Ser Ala Arg Asp lie Al a G u Arg Leu 360 365 ttc acc acc tct ggt gat acc gca aag Phe Thr Thr Ser G y Asp Thr Al a Lys 375 380 cac ctg cca ctg ctc gtg ttc act cca His Leu Pro Leu Leu Val Phe Thr Pro 390 395 ctg gcg ctg acc tgg ggt gca acc acc Leu Ala Leu Thr Trp G y Ala Thr Thr 410 gat acc gat gac atg atg cgc gaa gt c Asp Thr Asp Asp IVet IVet Arg G u Val 425 430 cct gag tac aac aag ggt gac atg at g Pro G u Tyr Asn Lys G y Asp IVet IVet 440 445 cct ggt gtt acc ggt aac acc aac atg Pro y Val Thr Gy Asn Thr Asn IVet 455 460 gac gac aca agg att gca aag ctc taal Asp Asp Thr Arg lie Ala Lys Leu 470 475 <210> 74 <211> 477 <212> PRT <213> Corynebacter i um gl utam cum <400> 74 IVet Gy Val Asp Arg Arg Thr Lys lie 1 5 Val Ala Ser Ala Asp Gy Ile Leu Arg 25 Val Ala Arg Leu Asn Phe Ser His G y 40 Asn Tyr Lys Trp Val Arg G u Ala Ala 55 Gy Ile Leu Ala Asp Leu n Gy Pro 70 Thr Asp y Ala Thr Val Trp G u Asn Val Asp Asp Val G u G y Thr His Asp 100 105 Asn Leu Ala Lys Asp Al a Lys Pro y 115 120 G y Lys Val G y Leu Val Cys Val Ser 130 135 gct Al a gt g Val gag Gu 400 ct g Leu cgt Ar g gtt Val cac Hi s cgt Ar g gct Al a 385 gca Al a tgt Cys gct Al a gtt Val gt c Val gca Al a 370 cgt Ar g gtt Val cca Pr o ctt Leu gca Al a 450 cac Hi s ttg Leu ct g Leu cgc Ar g cct Pr o tta Leu 435 ggt Gy ctt Leu gtt Val cac Hi s tct Ser gt c Val 420 gca Al a t cc Ser ctt Leu 1219 1267 1315 1363 1411 1459 1507 1554 465 :cgctt a aat ct t t caa aaa Val Cys Thr Leu G y Pr 10 Leu Val u Asp Gy M Asp His Pro Asp His G G u Lys Thr y Arg Al Lys lie Arg Leu Gy Ar Gy u Thr Ile Arg II 90 Arg Val Ser Thr Thr T 110 Asp Arg Leu Leu Val A 125 Val G u y Asn Asp V, 140 Page 98 ro 3t u a rg e yr sp Ial Gene Sequence 24-2-06.txt Cys u Val Val G u G y Gy Pro Val Ser Asn Asn Lys y Val Ser 145 150 155 160 Leu Pro Gy IVet Asp I e Ser Val Pro Ala Leu Ser G u Lys Asp Ile 165 170 175 Arg Asp Leu Arg Phe Al a Leu Lys Leu y Val Asp Phe lie Ala Leu 180 185 190 Ser Phe Val Arg Ser Pro Ala Asp Ala G u Leu Val His Lys lie IVet 195 200 205 Asp G u G u G y Arg Arg Val Pro Val Ile Ala Lys Leu G u Lys Pro 210 215 220 G u Ala Val Thr Ser Leu G u Pro lie Val Leu Ala Phe Asp Ala Val 225 230 235 240 Vet Val Al a Arg G y Asp Leu G y Val G u Val Pro Leu G u G u Val 245 250 255 Pro Leu Val n Lys Arg Ala lie On lie Ala Arg u Asn Ala Lys 260 265 270 Pro Val lie Val Ala Thr G n IVet Leu Asp Ser IVet Ile G u Asn Ser 275 280 285 Arg Pro Thr Arg Ala G u Ala Ser Asp Val Ala Asn Ala Val Leu Asp 290 295 300 G y Al a Asp Al a Val et Leu Ser G y G u Thr Ser Val G y Lys Asp 305 310 315 320 Pro His Asn Val Val Arg Thr IVet Ser Arg lie Val Arg Phe Ala G u 325 330 335 Thr Asp y Arg Val Pro Asp Leu Thr His lie Pro Arg Thr Lys Arg 340 345 350 Sy Val lie Ser Tyr Ser Ala Arg Asp lie Ala u Arg Leu Asn Ala 355 360 365 Arg Ala Leu Val Ala Phe Thr Thr Ser y Asp Thr Ala Lys Arg Val 370 375 380 Ala Arg Leu His Ser His Leu Pro Leu Leu Val Phe Thr Pro Asn G u 385 390 395 400 Ala Val Arg Ser G u Leu Ala Leu Thr Trp Gy Ala Thr Thr Phe Leu 405 410 415 Cys Pro Pro Val Ser Asp Thr Asp Asp IVet IVet Arg G u Val Asp Arg 420 425 430 Al a Leu Leu Al a IVet Pro G u Tyr Asn Lys G y Asp IVet IVet Val Val 435 440 445 Val Ala G y Ser Pro Pro G y Val Thr G y Asn Thr Asn IVet IIe His 450 455 460 Val His Leu Leu G y Asp Asp Thr Arg IIe Ala Lys Leu 465 470 475 <210> <211> 1980 <212> DNA Page 99 Gene Sequence 24-2-06.txt <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(1957) <223> RXN02675 <400> aagtgtttca ttggaacact tgcgctgcca actttttggt ttacgggcac aatgaaactg ttggatggaa tttagagtgt ttgtagctta aggagctcaa atg bet aat gag ttt Asn G u Phe cag G n gaa G u t cc Ser ctt Leu gga Gy aag Lys t at Tyr ct c Leu cca Pr o gaa G u 150 aac Asn gt c Val gac Asp gaa G u at t SIle gat Asp acc Thr acc Thr acc Thr gcc Al a ccc Pr o gag G u 120 tgc Cys gt c Val gca Al a acc Thr gcc Al a 200 ggt Gy cag G n gt g Val t ac Tyr gac Asp ttg Leu aat Asn gat Asp gcc Al a at g et ggc Gy gac Asp 170 gca Al a cca Pr o gca Al a gag G u aca Thr gtt Val ct c Leu act Thr 75 gtt Val gt c Val gaa G u gt c Val tt c Phe 155 gat Asp gaa G u aaa Lys cga Ar g at c SIle caa G n ggt Gy cgt Ar g acc Thr at c SIle gt c Val at t SIle acc Thr 140 gcc Al a gaa G u gaa G u gt a Val gt a Val act gaa ct c Thr G u Leu 15 cac agc gag Hi s Ser G u cgc aac ct c Arg Asn Leu ctg cag caa Leu G n G n gaa cca gca G u Pro Ala 80 gct ttc gca Al a Phe Al a 95 gcc ttc gaa Al a Phe G u 110 ctt ggc gaa Leu G y G u ccc acc gaa Pro Thr G u agc ggc atg Ser G y lbet 160 gtc tgg aag Val Trp Lys 175 ggc cgg gaa G y Arg G u 190 acc ggc gaa Thr G y G u cgc gac gaa Arg Asp u 1 gac Asp gcc Al a at g et cgc Ar g gt g Val ggt Gy gat Asp ccc Pr o gcc Al a 145 aat Asn cag G n at c SIle at c SIle gag G u at c l Ie cat Hi s ct c Leu cag G n gaa G u gcc Al a ct a Leu 130 gcc Al a ct a Leu at g let cgc Ar g gcc Al a 210 tta Leu ggg Sy t ac Tyr t cc Ser gcc Al a ggc G y gat Asp 115 ccg Pr o acc Thr gct Al a at c SIle gt c Val 195 cca Pr o at t I e cag G n gcg Al a tct Ser cgc Ar g cca Pr o 100 gag G u gat Asp gac Asp cgc Ar g gac Asp 180 agc Ser ggc Sy gac Asp ct a Leu gtc Val cat Hi s gtg Val ct c Leu ctt Leu att SIe act Thr att SIe at c SIe 165 aac Asn at g let gca Al a acc gga aaa gta ctg Thr G y Lys Val Leu Page 100 Gene Sequence 24-2-06.txt 215 220 225 acg ccc gca aaa ctg tgg atc acc gcc cac ggc tcc gaa cca gtc cca 835 Thr Pro Ala Lys Leu Trp lie Thr Ala His G y Ser G u Pro Val Pro 230 235 240 245 gcc ccc gaa agc ctg ccc ggt cgc ccc gct ctg ccg att gaa gtc acc 883 Ala Pro u Ser Leu Pro y Arg Pro Ala Leu Pro Ile G u Val Thr 250 255 260 cca gaa tgg ttc gac aaa cta gaa atc ggc agc gtc atc aac gtc cca 931 Pro G u Trp Phe Asp Lys Leu G u lie G y Ser Val lie Asn Val Pro 265 270 275 gac acc cgc gga tcc cgc cga gca ttc acc gtg acc agg gtt ttt gat 979 Asp Thr Arg y Ser Arg Arg Ala Phe Thr Val Thr Arg Val Phe Asp 280 285 290 ggc gcg gtc ctc gcc gaa ggc cca caa aaa gcc tac atc tcc aac ggc 1027 Sy Ala Val Leu Ala u Gy Pro On Lys Ala Tyr lie Ser Asn y 295 300 305 acc ctc ctg gaa cac aac tac gac cgc tcc cgg gtc tac ggc atc ccc 1075 Thr Leu Leu G u His Asn Tyr Asp Arg Ser Arg Val Tyr G y lie Pro 310 315 320 325 gcc gta gtt cag cgc atc aac ctc aaa gtc ggc gac cgc ctc atc ctt 1123 Ala Val Val G n Arg I e Asn Leu Lys Val y Asp Arg Leu Ile Leu 330 335 340 acc gac gaa gaa ctc acc tac gat cca tcc ctc gga tcc ggc cgc aca 1171 Thr Asp G u G u Leu Thr Tyr Asp Pro Ser Leu y Ser y Arg Thr 345 350 355 cca cgc atc agc tgc acc ctt cca caa gca gtc gat gca att aaa gtc 1219 Pro Arg lie Ser Cys Thr Leu Pro G n Ala Val Asp Ala lie Lys Val 360 365 370 ggg cac cgc gtg ctt ttc gac gac gga gcc atc gcc gca gtc tgc atc 1267 G y His Arg Val Leu Phe Asp Asp G y Ala IIe Ala Ala Val Cys IIe 375 380 385 gac aag acc tcc act gcc gac ggc cac aac gac gta gaa ttg gaa gtc 1315 Asp Lys Thr Ser Thr Al a Asp G y His Asn Asp Val G u Leu G u Val 390 395 400 405 acc cac gcc cgc cca caa ggc gta aac ctg gcc gca tac aag gga atc 1363 Thr His Ala Arg Pro n G y Val Asn Leu Ala Ala Tyr Lys G y IIe 410 415 420 aac ctc cca gac tcc gaa ctt cca ctc cca agc ctc act gaa gaa gac 1411 Asn Leu Pro Asp Ser G u Leu Pro Leu Pro Ser Leu Thr G u G u Asp 425 430 435 ctc caa cac ctg cgc ttt gtc gtgc aaa tac gcc gac atc gca gcc atc 1459 Leu G n His Leu Arg Phe Val Val Lys Tyr Ala Asp lie Ala Ala lie 440 445 450 tcc ttc atc cga aac gtc gcc gac gtg gaa tac ctc ctc caa gca ctc 1507 Ser Phe lie Arg Asn Val Ala Asp Val G u Tyr Leu Leu G n Ala Leu 455 460 465 gcc gac atc gga gat cca gta gcc gtc gaa cgc ctt ggc ctc gtc ctt 1555 Ala Asp Ile y Asp Pro Val Ala Val G u Arg Leu y Leu Val Leu 470 475 480 485 aaa atc gag acc atc cca ggc tac gaa ggc ctc gcc caa atc ctc ctg 1603 Lys Ile u Thr lie Pro y Tyr u y Leu Ala n lie Leu Leu Page 101 490 acc ggc at g cgc cac gaa Thr 31y I'b t Ar g His O31u 505 ctc gcc gtc gaa ctc ggc Leu Al a Val O31u Leu O31y 520 at c at g gcc ct t gcc gaa Il e I'b t Al a Leu Al a 03 u 535 caa gt c ct g gaa aac at g O31n Val Leu O31u Asn I'b t 550 555 at c acc gac gca gca at g Il e Thr Asp Al a Al a I'b t 570 aag gga cca cac atc aac Lys G31y Pr o Hi s Ilie Asn 585 cgc aaa ct t ggt gca t cc Ar g Lys Leu G31y Al a Ser 600 gt g aag agc t gg gaa gag Val Lys Ser Tr p 31u O31u 615 <210> 76 <211> 619 <21 2> PRT <21 3> Cor ynebact er i urn gi <400> 76 I'bt Asn 03 u Phe Asp 03 n 1 5 Asp 0 u Leu Il e Leu 0 u Ala lie Gy On Val Ser I'b t Hi s Ty r Al a Hi s Leu Ar g Leu Ser Ser Val G31y 70 Val O31n Al a Ar g Leu Lys G31y O31u G31y Pr o Leu Tyr 1 00 Asp Al a Asp 03 u Il e Leu 115 Pr o Leu Pr o Asp Thr Pr o 130 ene Sequence 24- 2- 06. t xt 495 aac t tc ggc at c at g at c gcc cgc As n Phe G31y I Ie I'b t IIe Ala Ar g 510 515 ttc gac cgc atg gca gaa gtc ccc Phe Asp Ar g I'b t Al a O31u Val Pr o 525 530 gcc gcc cac gtc cca acc atc ttg Al a Al a Hi s Val Pr o Thr Ilie Leu 540 545 gcc aaa aac gga ct C cca t ct cgc Al a Lys Asn G31y Leu Pr o Ser Ar g 560 gca ct t cgc gct gaa t gc gt c at g Al a Leu Ar g Al a O31u Cys Val I'b t 575 gac gcc at c aag gtc c t C acc gaa Asp Al a Ilie Lys Val Leu Thr O31u 590 595 caa cga aag agt agg ctg ctg ctg O31n Arg Lys Ser Arg Leu Leu Leu 605 610 t aact cacaa aggcgat tgg cgt 500 gga G31y c aa O31n gcc Al a gca Al a ct g Le u 580 at g vb t cgc Ar g O31u Ser As n O31n Pr o Ph e Ph e G31y Thr g ac As p ct g Le u ac c Thr g aa O31u 565 aac As n ag c Ser aag 1651 1 699 1 747 1 795 1 843 1891 1 939 1 980 ut ani cumn Asp Il e Leu Leu Asp 03 u 25 Pr o Thr Hi s 40 Ar g Thr Lys Al a Thr Ar g Al a Al a Ar g Pr o Pr o Ser 1 05 As p 03 u Hi s 120 Ser Cys I I e 135 O31n (31I 10 Val ThrI Ty r Val Asp Leu Leu Thr As n ValI 90 As p Val Al a 03 uI [Vb t Val Page 102 Lys5 Thr Al a G31y Thr G31y As p Le u 125 Le u Thr Hi s Ar g Le u O31u Al a Al a 110 Le u Pr o Al aAl aThr Asp Il e 0 u Leu 145 150 ene Sequence 24- 2- 06. txt Val Ar g G31y Phe Al a Lys Ser G31y Ntbt 155 160 As n O31n I Ie I Ie Th r 225 Ser Pr o ValI Thr Tyr 305 ValI As p G31y As p Al a 385 ValI Al a Le u As p Le u 465 Le u Al a Al a I Ie ValI 1 95 Pr o Ly s Pr o O31u As n 275 ValI Ser G31y Le u G31y 355 li1e ValI Le u Ly s O31u 435 Al a O31n Le u I Ie I Ie 1 65 As n vb t Al a Le u Pr a 245 Th r Pr a As p G31y Pr a 325 Le u Th r ValI I Ie ValI 405 li1e As p I Ie Le u Le u 485 Le u As n ValI As p O31u Thr 230 Al a Pr a As p G31y Th r 310 Al a Th r Pr a G31y As p 390 Thr As n Le u Ser Al a 470 Ly s Thr cys Hi s Le u ValI 215 Pr a Pr a O31u Thr Al a 295 Le u ValI As p Ar g Hi s 375 Ly s Hi s Le u O31n Ph e 455 As p I Ie (31y Al a Thr Al a 200 G31y Al a (31u Tr p Ar g 280 ValI Le u ValI (31u 360 Ar g Thr Al a Pr a Hi s 440 (31u Hi s ValI 1 85 G31y Ar g Ly s Ser Ph e 265 G31y Le u (31u O31n O31u 345 Ser ValI Ser Ar g As p 425 Le u Ar g O31y Thr Ar g 505 As p 1 70 Al a Pr a Al a Le u Le u 250 As p Ser Al a Hi s Ar g 330 Le u cys Le u Thr Pr a 410 Ser Ar g As n As p I Ie 490 As p O31u Ly s Ar g Tr p 235 Pr a Ly s Ar g O31u As n 315 li1e Thr Thr Ph e Al a 395 O31n O31u Ph e ValI Pr a 475 Pr a O31u O31u ValI ValI 220 O31y Le u Ar g O31y 300 Tyr As n Tyr Le u As p 380 As p O31y Le u ValI Al a 460 ValI O31y Thr ValI Ar g 205 Thr Thr Ar g O31u Al a 285 Pr a As p Le u As p Pr a 365 As p O31y ValI Pr a ValI 445 As p Al a Tyr Val Tr p Lys 1 O31y Ar g O31u 1 Thr 03 y 03 u Ar g As p O31u Al a Hi s 03 y 240 Pr o Al a Leu 255 1 1 e 03 y Ser 270 Phe Thr Val O3 n Lys Al a Ar g Ser Ar g 320 Lys Val (31y 335 Pr o Ser Leu 350 O3 n Al a Val O3 y Al a I I e Hi s As n As p 400 Asn Leu Al a 415 Leu Pr o Ser 430 Lys Tyr Al a Val O31u Tyr Val O31u Ar g 480 O3 u 03 y Leu 495 03 y IlIe N/t 510 Hi s u Asn Phe Page 103 lie Ala Arg Gy Asp Leu Ala 515 Su Val Pro n Leu Ile Met 530 535 Thr lie Leu Ala Thr G n Val 545 550 Pro Ser Arg Ala G u Ile Thr 565 Cys Val IVet Leu Asn Lys G y 580 Leu Thr G u IVet Ser Arg Lys 595 Leu Leu Leu Arg Lys Val Lys 610 615 <210> 77 <211> 386 <212> DNA <213> Corynebacter i um gl utar Gene Sequence 24-2-06.txt Val G u Leu y Phe Asp Arg 520 525 Ala Leu Ala G u Ala Ala Hi s 540 Leu G u Asn IVet Ala Lys Asn 555 Asp Ala Ala IVet Ala Leu Arg 570 Pro His Ile Asn Asp Ala lle 585 590 Leu y Ala Ser G n Arg Lys 600 605 Ser Trp u u bet Val Gy Al a 575 Lys Ser <220> <221> CDS <222> <223> FRXA02675 <400> 77 at c I e 1 cgc Ar g ccc Pr o ttg Leu cgc Ar g at g et gaa G u ct g Leu cgt at g Net gac Asp ct g Leu acc Thr gaa G u aac Asn agc Ser aag Lys 115 ggc Gy 5 gcc Al a at g Met gt c Val acc Thr gga Gy aaa Lys aag Lys cgc Ar g gaa G u ctt Leu gaa G u gca Al a cac Hi s ggt Gy tgg Tr p cac Hi s ct c Leu gca Al a 40 aac Asn gca Al a at c SIle gca Al a gaa G u 120 gaa G u ggc Gy gaa G u at g et at g bet aac Asn t cc Ser 105 gag G u aac ttc ggc atc atg atc Asn Phe Gy Ile IVet lie 10 ttc gac cgc atg gca gaa Phe Asp Arg IVet Ala G u gcc gcc cac gtc cca acc Ala Ala His Val Pro Thr gcc aaa aac gga ctc cca Ala Lys Asn Gy Leu Pro gca ctt cgc gct gaa tgc Ala Leu Arg Ala G u Cys 75 gac gcc atc aag gtc ctc Asp Ala lie Lys Val Leu 90 caa cga aag agt agg ctg G n Arg Lys Ser Arg Leu 110 t aact cacaa aggcgat t gg gcc Al a gtc Val at c SIe tct Ser gtc Val acc Thr ctg Leu Page 104 Gene Sequence 24-2-06. txt <210> 78 <211> 121 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 78 lie Leu et Thr G y et Arg His G u 1 5 Arg y Asp Leu Ala Val G u Leu y 25 Pro n Leu Ile IVet Ala Leu Ala u 40 Leu Ala Thr G n Val Leu G u Asn IVet Arg Ala u Ile Thr Asp Ala Ala IVet IVet Leu Asn Lys G y Pro His lie Asn G u IVet Ser Arg Lys Leu G y Al a Ser 100 105 Leu Arg Lys Val Lys Ser Trp G u G u 115 120 Asn 10 Phe Al a Al a Al a Asp 90 G n I le et Val Gy Al a Lys Ser bet Al a Pr o Leu G u Val Ar g 110 I e G u Thr Pr o Cys Leu Leu <210> 79 <211> 1522 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1522) <223> FRXA02695 <400> 79 aagtgtttca ttggaacact tgcgctgcca actttttggt ttggatggaa tttagagtgt ttgtagctta aggagctcaa cag G n gaa G u t cc Ser ctt Leu gga G y at t SIle gat Asp acc Thr acc Thr acc Thr cag G n gt g Val t ac Tyr gac Asp ttg Leu gag G u aca Thr gtt Val ct c Leu act Thr 75 at c SIle caa G n ggt G y cgt Ar g acc Thr aag Lys act Thr gcc Al a 45 ggc G y acc Thr act Thr cac Hi s cgc Ar g ct g Leu gaa G u gaa ct c G u Leu 15 agc gag Ser G u aac ct c Asn Leu cag caa G n n cca gca Pro Al a 80 Page 105 ttacgggcac aatgaaactg atg aat gag ttt gac IVet Asn G u Phe Asp 1 gac gag tta att cta Asp G u Leu Ile Leu gcc atc ggg cag gtc Ala IIe G y G n Val atg cat tac gcg cat Net His Tyr Ala His cgc ctc tcc tct gtg Arg Leu Ser Ser Val gtg cag gcc cgc ctc Val G n Ala Arg Leu Gene Sequence 24-2-06.txt aag Lys t at Tyr ct c Leu cca Pr o gaa G u 150 aac Asn gt c Val gac Asp gaa G u acg Thr 230 gcc Al a cca Pr o gac Asp ggc G y acc Thr 310 gcc Al a acc Thr gcc Al a ccc Pr o gag G u 120 tgc Cys gt c Val gca Al a acc Thr gcc Al a 200 ggt Gy gca Al a gaa G u tgg Tr p cgc Ar g 280 gt c Val ct g Leu gtt Val gaa Gu aat Asn gat Asp gcc Al a at g et ggc G y gac Asp 170 gca Al a cca Pr o gca Al a ct g Leu ct g Leu 250 gac Asp t cc Ser gcc Al a cac Hi s cgc Ar g 330 ct c Leu gtt atc gga gct Val I e Gy Al a gtc gtc gat gcc Val Val Asp Al a 110 gaa att ctc ctt G u Ile Leu Leu 125 gtc acc ctg ccc Val Thr Leu Pro 140 ttc gcc aaa agc Phe Ala Lys Ser 155 gat gaa acc gt c Asp G u Thr Val gaa gaa gtt ggc G u u Val y 190 aaa gta cgc acc Lys Val Arg Thr 205 cga gta acc cgc Arg Val Thr Arg 220 tgg atc acc gcc Trp Ile Thr Ala 235 ccc ggt cgc ccc Pro G y Arg Pr o aaa ct a gaa at c Lys Leu G u Ile 270 cgc cga gca ttc Arg Arg Ala Phe 285 gaa ggc cca caa G u G y Pro G n 300 aac tac gac cgc Asn Tyr Asp Arg 315 at c aac ctc aaa Ile Asn Leu Lys acc tac gat cca Thr Tyr Asp Pro 350 ttc gca ggt gaa ggc Phe Ala G y G u G y gaa Gu gaa Gu gaa Gu at g Let 160 aag Lys gaa Gu gaa Gu gaa Gu ggc Gy 240 ct g Leu agc Ser gt g Val gcc Al a cgg Ar g 320 ggc G y ct c Leu gat Asp ccc Pr o gcc Al a 145 aat Asn cag G n at c SIle at c SIle acc Thr 225 t cc Ser ccg Pr o gt c Val acc Thr t ac Tyr 305 gt c Val gac Asp gga Gy gcc Al a ct a Leu 130 gcc Al a ct a Leu at g Met cgc Ar g gcc Al a 210 gga Gy gaa G u at t SIle at c SIle agg Ar g 290 at c SIle t ac Tyr cgc Ar g t cc Ser gat Asp 115 ccg Pr o acc Thr gct Al a at c SIle gt c Val 195 cca Pr o aaa Lys cca Pr o gaa G u aac Asn 275 gtt Val t cc Ser ggc G y ct c Leu ggc Gy 355 cca ctt Pro Leu 100 gag att S u Ile gat act Asp Thr gac att Asp I e cgc at c Arg Ile 165 gac aac Asp Asn 180 agc at g Ser bet ggc gca G y Al a gta ct g Val Leu gtc cca Val Pro 245 gtc acc Val Thr 260 gtc cca Val Pro ttt gat Phe Asp aac ggc Asn y atc ccc Ile Pro 325 atc ctt I e Leu 340 cgc aca Arg Thr 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 Page 106 cca cgc atc agc tgc acc ctt Pro Arg lie Ser Cys Thr Leu 360 ggg cac cgc gtg ctt ttc gac Gy His Arg Val Leu Phe Asp 375 380 gac aag acc tcc act gcc gac Asp Lys Thr Ser Thr Ala Asp 390 395 acc cac gcc cgc cca caa ggc Thr His Ala Arg Pro G n y 410 aac ctc cca gac tcc gaa ctt Asn Leu Pro Asp Ser G u Leu 425 ctc caa cac ctg cgc ttt gtc Leu G n His Leu Arg Phe Val 440 tcc ttc atc cga aac gtc gcc Ser Phe lie Arg Asn Val Ala 455 460 gcc gac atc gga gat Al a Asp I e G y Asp 470 <210> <211> 474 <212> PRT <213> Corynebacter i um gl ut ar <400> IVet Asn G u Phe Asp G n Asp 1 5 Asp G u Leu Ile Leu G u Leu Ala Ile Gy On Val Ser Pro IVet His Tyr Ala His Leu Arg 55 Arg Leu Ser Ser Val G y Al a 70 Val G n Ala Arg Leu Lys Ala Sy G u y Pro Leu Tyr Pro 100 Asp Ala Asp G u Ile Leu Asp 115 Pro Leu Pro Asp Thr Pro Ser 130 135 Ala Ala Thr Asp Ile G u Leu Gene Sequence 24-2-06.txt cca caa gca gtc gat gca att Pro n Ala Val Asp Ala lie 365 370 gac gga gcc atc gcc gca gtc Asp G y Ala lIe Ala Ala Val 385 ggc cac aac gac gta gaa ttg Sy His Asn Asp Val G u Leu 400 gta aac ctg gcc gca tac aag Val Asn Leu Ala Ala Tyr Lys 415 cca ctc cca agc ctc act gaa Pro Leu Pro Ser Leu Thr G u 430 435 gtc aaa tac gcc gac atc gca Val Lys Tyr Ala Asp lie Ala 445 450 gac gtg gaa tac ctc ctc caa Asp Val G u Tyr Leu Leu G n 465 aaa Lys tgc Cys gaa G u gga G y 420 gaa G u gcc Al a gca Al a 1219 1267 1315 1363 1411 1459 1507 1522 Ile Leu Gn u lie Lys Thr u Leu G u 25 Hi s Lys Ar g Ar g Ser 105 Hi s SIle Ar g 10 Val Thr G n Tyr Val G y Asp Leu Arg Leu Thr Thr 75 Asn Val lle 90 Asp Val Val Ala G u Ile et Val Thr 140 G y Phe Al a Page 107 Thr Al a Sy Thr Sy Asp Leu 125 Leu Lys Hi s Ar g Leu G u Al a Al a 110 Leu Pr o Ser Ser Asn G n Pr o Phe Phe Sy Thr Sy G u Leu G n Al a Al a G u G u G u bet Gene Sequence 24-2-06.txt 155 Asp G u Thr Val Asn G n SIle SIle Thr 225 Ser Pr o Val Thr Tyr 305 Val Asp Sy Asp Al a 385 Val Al a Leu Asp Leu 465 Al a SIle Val 195 Pr o Lys Pr o G u Asn 275 Val Ser Sy Leu Sy 355 I le Val Leu Lys G u 435 Al a G n l Ie 165 Asn Met Al a Leu Pr o 245 Thr Pr o Asp Sy Pr o 325 Leu Thr Val SIle Val 405 I le Asp l Ie Cys Hi s Leu Val 215 Pr o Pr o G u Thr Al a 295 Leu Val Asp Ar g Hi s 375 Lys Hi s Leu G n Phe 455 Al a Thr Al a 200 Sy Al a G u Tr p Ar g 280 Val Leu Val G u SIle 360 Ar g Thr Al a Pr o Hi s 440 I le His Asp 170 Val Ala 185 G y Pro Arg Ala Lys Leu Ser Leu 250 Phe Asp 265 G y Ser Leu Al a G u Hi s G n Arg 330 G u Leu 345 Ser Cys Val Leu Ser Thr Arg Pro 410 Asp Ser 425 Leu Arg Arg Asn u Lys Ar g Tr p 235 Pr o Lys Ar g G u Asn 315 I le Thr Thr Phe Al a 395 G n G u Phe Val Ar g 205 Thr Thr Ar g G u Al a 285 Pr o Asp Leu Asp Pr o 365 Asp Sy Val Pr o Val 445 Gy 190 Thr Ar g Al a Pr o SIle 270 Phe G n Ar g Lys Pr o 350 G n Sy Hi s Asn Leu 430 Lys Tr p 175 Ar g Sy Asp Hi s Al a 255 Sy Thr Lys Ser Val 335 Ser Al a Al a Asn Leu 415 Pr o Tyr Lys G u G u G u Sy 240 Leu Ser Val Al a Ar g 320 Sy Leu Val SIe Asp 400 Al a Ser Al a Val Ala Asp Val u Tyr Al a Leu Al a 470 Asp Ile G y Asp <210> 81 <211> 2022 <212> DNA <213> Corynebacter i um gl utam cum Page 108 Gene Sequence 24-2-06.txt <220> <221> CDS <222> (101)..(1999) <223> RXA00682 <400> 81 ataggcacct tcgatttcag ctcaatcacc gtcgcaatga cgcatctttt cgtcgaaaag catctaaaag gagtttgacc ccggcacgaa gtaaaaccac gct aat aaa Al a Asn Lys ttc Phe gaa G u caa G n gt g Val ct c Leu at c SIle gt g Val at c SIle gag G u cca Pr o 150 aaa Lys ct c Leu t ac Tyr aag Lys ct g Leu aat Asn aag Lys caa G n ttc Phe gaa G u cac Hi s 120 gt a Val cct Pr o ggc Sy t ac Tyr gct Al a 200 t cc Ser ccg Pr o aaa Lys ttt Phe aac Asn gt g Val cag G n ttc Phe gga Gy t ac Tyr gaa G u 170 aac Asn ct a Leu gat Asp tac Ty r tt c Phe gaa Gu gct Al a 75 cat Hi s at t SIle caa G n acc Thr gt g Val 155 gt a Val tgg Tr p gat Asp ct Le ta Ty tg Tr ac Th 6 cg Ar ct Le gc Al aa As at II 14 cc Pr ct Le ca G tt Ph t cca gtg ccc u Pro Val Pro 15 c ctc gtt ttc r Leu Val Phe g ttc tgc gat p Phe Cys Asp 45 t atc ggt ggt r Ile Gy G y 0 g cat ttg atg g His Leu IVet g gga tac ct c u G y Tyr Leu 95 g gaa cgc gtc a u Arg Val 110 c tgg gag cca n Trp G u Pro 125 c aat gag ctg e Asn 0 u Leu 0 t atc gat gat o Ile Asp Asp c atg gaa aac u Iet u Asn 175 a tac cac ttt n Tyr His Phe 190 c ttc aat ttc e Phe Asn Phe 205 t tcg atg atg e Ser et bet aag Lys caa G n t ca Ser gaa Gu at c I e t at Tyr ccc Pr o at g bet gaa G u at t SIle 160 ttc Phe gag G u tgc Cys gtt Val gct Al a aag Lys cac Hi s gct Al a aat Asn t cc Ser ttc Phe gca Al a 130 ct a Leu t cc Ser cgg Ar g ct c Leu gaa G u 210 ggc G y gaa G u ct c Leu tgg Tr p gt a Val gcc Al a cct Pr o cag G n 115 aat Asn gaa G u gga G y ct c Leu aac Asn 195 gt c Val gt g Val ggt G y at g bet cca Pr o aag Lys aat Asn att SIe 100 gaa G u tgg Tr p ttc Phe aaa Lys att SIe 180 ttg Leu ttc Phe gat Asp gat atc cct gat caa tca at Asp lie Pro Asp Gn Ser II 220 Page 109 Gene Sequence 24-2-06.txt gag G u 230 gt c Val gct Al a gcg Al a aat Asn cca Pr o 310 acc Thr gt g Val gat Asp cat Hi s gt a Val 390 gag G u ttc Phe ggc Gy acc Thr gag Gu 470 gaa Gu ttc Phe ctt Leu ttg Leu tgg Tr p 280 ttc Phe ggg Gy t cc Ser gaa G u aaa Lys 360 ttc Phe gaa G u gac Asp ct g Leu at c SIle 440 ttg Leu at c SIle gt g Val ccc gat gat gaa Pro Asp Asp G u 235 ctg caa act cac Leu G n Thr His 250 gct atc gca aag Ala I e Ala Lys gaa gca caa gat G u Al a G n Asp 285 ggt cac gat aaa G y Hi s Asp Lys 300 atc gcg gat tac I e Al a Asp Tyr 315 ccg aaa gat gaa Pro Lys Asp G u 330 cgc gac ctt ttg Arg Asp Leu Leu ggc ctc gct gct G y Leu Al a Al a 365 atc gag cac tgg Ile u His Trp 380 tcc cgc act ctc Ser Arg Thr Leu 395 ttg tac ctc aac Leu Tyr Leu Asn 410 act gcg tgg ggt Thr Al a Trp y tgg ccg gaa gaa Trp Pro G u G u 445 act gcc cga cca Thr Al a Arg Pro 460 gaa cct ttc acc G u Pro Phe Thr 475 tca tgg ttg ggc Ser Trp Leu y 490 cta aag att ctg Leu Lys Ile Leu gca cag cta gcg Ala G n Leu Ala ttt Phe gca Al a 270 ccg Pr o t ac Tyr at c SIle ct c Leu gat Asp 350 act Thr acc Thr cag G n cgc Ar g gt c Val 430 at t SIle gcg Al a cgc Ar g aat Asn gcc Al a 255 gaa G u tgg Tr p tgg Tr p cgc Ar g at c SIle 335 gga Sy gca Al a at g bet ggc Sy act Thr 415 ggc G y gag G u cca Pr o at g Net gac Asp 495 240 aac Asn ggc G y ttc Phe at c SIle cgc Ar g 320 gca Al a gaa G u t ac Tyr t ca Ser t ac Tyr 400 gaa G u gca Al a cga Ar g gct Al a ct c Leu 480 gag G u ccg Pr o ggc G y aac Asn gag G u 305 ct a Leu gaa Gu caa G n ccc Pr o gt a Val 385 ggt Sy gt c Val ccc Pr o aga Ar g ctt Leu 465 tgg Tr p gat Asp gcg Al a ttc Phe 290 cac Hi s gat Asp aag Lys ct c Leu t at Tyr 370 ttt Phe ttc Phe cgc Ar g ggt G y aaa Lys 450 aac Asn gga G y gat Asp aca Thr 275 acc Thr ct g Leu gaa G u gaa Gu gcg Al a 355 gt g Val tgg Tr p tgg Tr p gat Asp ggt G y 435 gca Al a act Thr at c SIle ccg Pr o 260 tgg Tr p gt c Val gaa G u ggc G y cgc Ar g 340 cag G n gaa G u cgc Ar g gag G u gt c Val 420 cca Pr o at t l Ie cct Pr o acc Thr 245 caa G n at a SIe ggt G y ctt Leu caa G n 325 gt g Val ttt Phe aac Asn aaa Lys aac Asn 405 ct c Leu at t SIe gt c Val cca Pr o acc Thr 485 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 gat gcc gaa gaa gga Asp Al a u u y 500 Page 110 Gene Sequence 24-2-06.txt acc ctt aaa ggc atg gct gca tcc cct ggt g Thr Leu Lys Gy Met Al a Ala Ser Pro Gy V 505 510 cga gta att ctc agc gca gat gac ctt tca g Arg Val Ile Leu Ser Ala Asp Asp Leu Ser G 520 525 atc ctc gtt gcc cct gta aca gca cct tct t Ile Leu Val Ala Pro Val Thr Ala Pro Ser T 535 540 aaa atc aag gca aca gtc act gat att ggt g Lys Ile Lys Ala Thr Val Thr Asp lie Gy G 550 555 5 gcg atc gtg tgc cgc gaa tac ggc ttg ccg g Ala lie Val Cys Arg u Tyr y Leu Pro A 570 575 gct gca tcc acc acc atc aaa acc ggc gat t Ala Ala Ser Thr Thr Ile Lys Thr y Asp T 585 590 acc aag ggc aag gtt gtc att gtt gat cca g Thr Lys G y Lys Val Val Ile Val Asp Pro A 600 605 gga ccc ggc gcg cac agc cat gcg cac tca g G y Pro G y Ala His Ser His Ala His Ser V 615 620 gat aca cat gcc tagtccacgc actgttctta tca Asp Thr His Ala 630 <210> 82 <211> 633 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 82 IVet Ala Asn Lys Ser Phe Pro Lys Pro Ser A 1 5 10 Gy Ala u y Trp u Asp Leu Tyr Pro T Asp Lys Leu IVet Asp n G u Asn G u Lys P 40 G n His Trp Pro Thr Val Phe Lys Pro Phe G 55 Phe Ala Val Lys Cys Leu Gy G n Tyr Asn A 70 Pro Asn Ala Asn y I e G u Phe Arg Val H 90 IVet Ser Pro lie Pro Val Pro u Asp Gn I 100 105 IVet Phe n G u Arg Ile Thr His Tyr Phe G 115 120 Leu Ala Asn Trp Lys G u Arg Val Leu y T Page tg gtg gaa al Val G u ggc tac gct G y Tyr Al a 515 aa lu gg rp gc ly 60 ct I a ac yr at sp ta al at c SIle ggc G y 545 at g Net gtt Val ct c Leu gcg Al a gca Al a 625 Leu Tyr Tr p Thr Ar g Leu Al a Asn SIle cag G n 530 cca Pr o at g bet act Thr aag Lys cca Pr o 610 gca Al a Pr o Leu Phe I e Hi s Sy G u Tr p 125 Asn gat Asp ttt Phe cat Hi s act Thr 580 gat Asp at c SIle ggg Sy Pr o Phe Asp Sy et Leu Val Pr o Leu gaa G u ggc G y gct Al a 565 ggc G y gga G y gaa Gu gt g Val Lys G n Ser G u I e Tyr Pr o bet G u 1651 1699 1747 1795 1843 1891 1939 1987 2022 sp yr he lu I a 75 is le In hr 111 Gene Sequence 24-2-06. txt 140 Pro Ile Asp Asp Ser 145 Val Asp Phe Lys G n 225 Leu Pr o Sy Asn G u 305 Leu G u G n Pr o Val 385 Sy Val Pr o Ar g Leu 465 Tr p G u Gy Leu Asn 195 Val Val G n Asp Thr 275 Thr Leu G u G u Al a 355 Val Tr p Tr p Asp Sy 435 Al a Thr SIle Lys Al a 165 G n Sy Pr o let Al a 245 G n SIle Gy Leu G n 325 Val Phe Asn Lys Asn 405 Leu SIle Val Pr o Thr 485 Pr o 150 Lys Leu Ty r Asp G u 230 Val Al a Al a Asn Pr o 310 Thr Val Asp Hi s Val 390 G u Phe Sy Thr G u 470 G u Leu Asp Al a I e I e 215 Leu Asp Thr Ar g Sy 295 Leu SIle G u Al a Asn 375 Ar g Asp Asp Thr Al a 455 Ser G n Pro Asp Tyr Thr G n 185 Tyr Asp Ar g Sy Al a 265 G u Tyr Ty r Ar g Tyr 345 Cys Tyr Leu Met Al a 425 I le Lys Thr G n Val 155 Val Tr p Asp Ser Asp 235 G n l Ie Al a Hi s Al a 315 Lys Asp Leu G u Ar g 395 Tyr Al a Pr o Al a Pr o 475 Leu G n Phe SIle 220 Asp Thr Al a G n Asp 300 Asp Asp Leu Al a Hi s 380 Thr Leu Tr p G u Ar g 460 Phe Net Tyr Phe 205 Ser G u Hi s Lys Asp 285 Lys Tyr G u Leu Al a 365 Tr p Leu Asn Sy G u 445 Pr o Thr Asn 175 Phe Phe let Lys Al a 255 G u Tr p Tr p Ar g SIle 335 Sy Al a let Sy Thr 415 Sy G u Pr o let SIe 160 Phe G u Cys Val SIe 240 Asn Sy Phe SIe Ar g 320 Al a G u Tyr Ser Tyr 400 G u Al a Ar g Al a Leu 480 Ser Trp Leu y Asn 490 Asp u 495 Page 112 Gene Sequence 24 Asp Ala Gu Gu Gy Thr Leu Lys Gy IVet Ala 500 505 Val G u y Tyr Ala Arg Val lie Leu Ser Ala 515 520 Ile n G n Asp Gu Ile Leu Val Ala Pro Val 530 535 Sy Pro lie Phe y Lys Ile Lys Ala Thr Val 545 550 555 et et Ser His Ala Al a le Val Cys Arg G u 565 570 Val Thr y Thr Gy Al a Ala Ser Thr Thr lie 580 585 Leu Lys Val Asp G y Thr Lys G y Lys Val Val 595 600 Ala Pro Arg lie G u G y Pro G y Ala His Ser 610 615 Ala Ala His G y Val Asp Thr His Ala 625 630 <210> 83 <211> 1215 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1192) <223> RXA00683 <400> 83 ggacaaagct atcgggttcc gggagaacct ctccttccgc gacttggaac gcttaacgct ttattaaata aggagacacc -2-06. txt Al a Ser Pro 510 Asp Asp Leu 525 Thr Ala Pro 540 Thr Asp Ile Tyr G y Leu Lys Thr G y 590 Ile Val Asp 605 Hi s Al a Hi s 620 Sy Ser Ser Sy Pr o 575 Asp Pr o Ser aac Asn gaa Su gga G y gaa G u aac Asn at c SIle ccg Pr o ct c Leu ccc Pr o at c SIle ct c Leu cgc Ar g gt c Val ggc G y cca Pr o gaa G u gca Al a gag G u cag G n aag Lys cct Pr o gca Al a gaa G u 75 ct g Leu cgc Ar g ggc Sy gga G y ggg G y gat Asp tgc Cys ttc Phe gct Al a ttt Phe 45 gtt Val gtt Val agt Ser gat Asp t ca Ser gt g Val gct Al a aag Lys ctt Leu gaa ggc Su G y 15 ct a gt c Leu Val gt c act Val Thr gaa cac G u Hi s gaa gt g G u Val 80 gac gtt Asp Val 95 Page 113 gtccccactt ctgttcccgt atg acc aac agt ttg IVet Thr Asn Ser Leu 1 ctg gat cct gtt cta Leu Asp Pro Val Leu acc atg aca gat gct Thr IVet Thr Asp Ala act gcc agc ttt gat Thr Ala Ser Phe Asp atc gat aaa ttc cta Ile Asp Lys Phe Leu gat cga gtt tct gcg Asp Arg Val Ser Ala cca gag aat gct cgt Pro G u Asn Ala Arg 100 Gene Sequence 24-2-06.txt ttc gca gtg cac cag gct tat cgc gat ctc atg gaa cga tgc ggt ggc 451 Phe Ala Val His G n Al a Tyr Arg Asp Leu Iet G u Arg Cys G y G y 105 110 115 gac gtc ccg gtt gct gtc cgg tca tcg gcc act gcc gaa gat ctg ccc 499 Asp Val Pro Val Ala Val Arg Ser Ser Ala Thr Ala G u Asp Leu Pro 120 125 130 gat gct tcc ttc gca ggg caa cag gac acc tat ctg tgg caa gtc ggt 547 Asp Ala Ser Phe Ala G y G n n Asp Thr Tyr Leu Trp G n Val y 135 140 145 ttg agc gct gtc act gaa cac atc cgt aaa tgc tgg gct tcg ctg ttc 595 Leu Ser Ala Val Thr G u His lie Arg Lys Cys Trp Ala Ser Leu Phe 150 155 160 165 act tcc cgt gcc att atc tac cgt ctg aaa aac aac atc ccc aat gag 643 Thr Ser Arg Ala lie Ile Tyr Arg Leu Lys Asn Asn lie Pro Asn G u 170 175 180 ggc ctc tcc atg gcg gta gtt gtt caa aaa atg gtc aac tct cgt gtc 691 Gy Leu Ser Met Ala Val Val Val G n Lys et Val Asn Ser Arg Val 185 190 195 gca ggc gtg gca atc act atg aat cct tcc aac ggc gac cgc tcg aag 739 Al a G y Val Al a I e Thr IVet Asn Pro Ser Asn G y Asp Arg Ser Lys 200 205 210 atc acc atc gat tcc tca tgg ggt gtt ggt gaa atg gtg gtc tca ggt 787 Ile Thr Ile Asp Ser Ser Trp y Val y u et Val Val Ser y 215 220 225 gaa gtg aca cca gac aat atc ttg ctg gac aag atc acg ctg cag gtt 835 G u Val Thr Pro Asp Asn Ile Leu Leu Asp Lys Ile Thr Leu G n Val 230 235 240 245 gtc tcc gaa cac att gga agc aaa cac gct gaa ctc atc ccc gat gcc 883 Val Ser u His Ile y Ser Lys His Ala u Leu lie Pro Asp Ala 250 255 260 acc agt gga agc ctc gtg gaa aag ccc gtt gat gaa gaa cgc gca aac 931 Thr Ser y Ser Leu Val G u Lys Pro Val Asp G u G u Arg Ala Asn 265 270 275 cgc cgc agt ct g act gat gag gaa atg ctc gct gtg gca caa atg gct 979 Arg Arg Ser Leu Thr Asp G u G u Iet Leu Ala Val Ala G n Iet Ala 280 285 290 aag cgt gca gaa aaa cac tac aag tgc cca caa gat atc gaa tgg gcg 1027 Lys Arg Ala G u Lys His Tyr Lys Cys Pro G n Asp lie G u Trp Ala 295 300 305 ctg gac gct gat ctg cca gat gga gaa aac ctt ctg tta ttg caa tcc 1075 Leu Asp Ala Asp Leu Pro Asp G y G u Asn Leu Leu Leu Leu G n Ser 310 315 320 325 cgc ccg gaa act atc cac tcc aac ggt gtg aag aag gaa acc cca act 1123 Arg Pro G u Thr lie His Ser Asn y Val Lys Lys G u Thr Pro Thr 330 335 340 ccg cag gct gcc aaa acc ata ggc acc ttc gat ttc agc tca atc acc 1171 Pro G n Ala Ala Lys Thr lie y Thr Phe Asp Phe Ser Ser lie Thr 345 350 355 gtc gca atg acc ggc acg aag taaaaccacc gcatcttttc gtc 1215 Val Ala \et Thr G y Thr Lys 360 Page 114 Gene Sequence 24-2-06.txt <210> 84 <211> 364 <212> PRT <213> Corynebacteri um gl ut arr cum <400> 84 Net 1 Leu Thr Thr SIle Asp Pr o G u Al a Leu 145 Tr p Asn Val Gy et 225 I le Leu G u Val Asp 305 Thr Asn Ser Asp Pro Val 2C IVet Thr Asp Al a Ser Phe Asp Lys Phe Arg Val Ser G u Asn Al Arg Cys G 115 G u Asp LeL 130 Trp n Val Al a Ser LeL li e Pro Asr 18C Asn Ser Arc 195 Asp Arg Ser 210 Val Val Ser Thr Leu G r li e Pro Asp 26C G u Arg Al 275 Ala G n lVet 290 lie G u Tr Leu Asn 5 Leu G u Al a G y Asp u Leu Asn 70 Ala I e Arg Phe Sy Asp Pro Asp G y Leu 150 Phe Thr 165 G u G y Val Ala Lys II e 3 y 3 u 230 Val Val 245 Al a Thr Asn Arg Al a Lys Al a Leu 310 SIle Val let Phe Asp SIle Al a Val Al a 135 Ser Ser Leu Gy Thr 215 Val Ser Ser Ar g Ar g 295 Asp Pr o Leu Pr o 40 I le Leu Ar g Val Pr o 120 Ser Al a Ar g Ser Val 200 I le Thr G u Gy Ser 280 Al a Al a Phe Gy Val Ar g Asp Asp Hi s 105 Val Phe Val Al a let 185 Al a Asp Pr o Hi s Ser 265 Leu G u Asp Val 10 Gy Pr o G u Al a G u 90 G n Al a Al a Thr l Ie 170 Al a l Ie Ser Asp SIle 250 Leu Thr Lys G n Lys Pr o Al a G u Leu Al a Val Gy G u 155 I le Val Thr Ser Asn 235 Gy Val Asp Hi s Phe Al a Phe Val Val Ser Ar g Ser 125 G n SIle Ar g Val Asn 205 Sy Leu Lys Lys G u 285 Lys G u Leu Val G u G u Asp Leu Al a Thr Lys Lys 175 Lys Ser Sy Asp Al a 255 Val Leu Pr o Leu Pro Asp y G u Asn Leu Page 115 Gene Sequence 24- 2- 06. txt Leu Leu Leu G n Ser Arg Pro G u Thr lie His Ser Asn y Val Lys 325 330 335 Lys G u Thr Pro Thr Pro G n Ala Ala Lys Thr I e y Thr Phe Asp 340 345 350 Phe Ser Ser Ile Thr Val Al a Net Thr G y Thr Lys 355 360 <210> <211> 1860 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1837) <223> RXN00635 <400> ctggcaggcg ggcgaagcgt ggcaacaact ggaatttaag aagttaggca acacaatagc cataacgttg aggagttcag gca Al a t at Tyr caa G n ttt Phe gct Al a gat Asp ccg Pr o at t SIle gag G u ggt G1y 150 caa G n ttg Leu gat Asp gcc Al a tct Ser cat Hi s gcc Al a ttt Phe 120 ggt gty ggt G y at t I e ggt Sy gag Su gcg Al a ggt Sy aat Asn at t l Ie gaa G u cgc Ar g tcg Ser act Thr agc Ser gt g Val tcg Ser gga G y gcg Al a tcg Ser tct Ser ttg Leu 140 gt a Val gaa G u aat Asn gtt Val at c SIle aca Thr gt g Val ttc Phe 110 t ac Tyr cac Hi s at t SIle gct Al a 15 ccg Pr o cga Ar g act Thr cac Hi s ttg Leu 95 ttc Phe tgc Cys gcg Al a cct Pr o caa G n at c SIle aat Asn ggg G y ct g Leu 80 gcc Al a cag G n gag G u at t l Ie ggt 10y 160 agcacaattg aagtcgcacc atg gca cac agc tac 11! et Ala His Ser Tyr 1 ggt gtg aag cga att 16 Sy Val Lys Arg lie gtg gat gct gtc cgc 21 Val Asp Ala Val Arg gag gaa gcg gcg gcg Su G u Al a Al a Al a gag ctg gca gta tgt G u Leu Ala Val Cys att cag ggt ctt tat Ile n G y Leu Tyr atc gct agc cat att lle Ala Ser His Ile 100 gaa acg cat ccg gag Su Thr His Pro u 115 atg gtg aat ggt ggt 49! IVet Val Asn y y 130 cag tcc acc atg gcg 54 G n Ser Thr IVet Ala 145 gat atc gct aag gaa 59! Asp Ile Ala Lys G u 165 att tct tct ggc act 64; gac gca ggt gac ggt act tat tcc aat tcc act Page 116 Gene Sequence 24-2-06.txt Ser Asn Ser Thr lie Ser I Asp Al a y Asp G y Thr Tyr 170 cct Pr o at t SIle aat Asn at c SIle 230 ttt Phe gcg Al a t at Tyr ggt Sy gat Asp 310 aca Thr ttg Leu cct Pr o aag Lys gcg Al a 390 ttc Phe caa G n gt g Val aac Asn 200 cgc Ar g cat Hi s gt c Val aat Asn gat Asp 280 cac Hi s gct Al a cgt Ar g tcg Ser cac Hi s 360 gcg Al a t ac Tyr cac Hi s gtt Val ccg Pr o aag Lys cag G n ct g Leu at g Net 250 gcg Al a ctt Leu ggt Sy aca Thr ttc Phe 330 gt a Val gaa G u ttt Phe gag G u acg Thr 410 cga Ar g cct Pr o gt c Val ttg Leu 220 ggt Gy ggc Gy ct g Leu aaa Lys cgt Ar g 300 gaa G u gat Asp acg Thr gtt Val gt g Val 380 ccg Pr o gct Al a cgc Ar g gag G u 190 ttg Leu ttg Leu cag G n ctt Leu at t SIle 270 aac Asn acg Thr at t SIle at g et aca Thr 350 tct Ser acc Thr gga Gy gcg Al a gt g Val 430 175 gct Al a ttc Phe gcg Al a t ac Tyr ggt Gy 255 ct a Leu gtt Val gt g Val ttg Leu ct c Leu 335 cat Hi s at t SIle ggc Gy acg Thr ttg Leu 415 at c SIle gca Al a tgc Cys gag G u at c lile 240 t ac Tyr ttg Leu gcc Al a aag Lys cct Pr o 320 aag Lys aac Asn ttg Leu at g let cgc Ar g 400 cct Pr o gcg Al a gcg Al a ggt Gy aag Lys 225 cag G n ggc G y ggt G y cag G n t at Tyr 305 cat Hi s gca Al a gt c Val aac Asn tgc Cys 385 gac Asp cat Hi s at g let Ser G y Thr 180 ct g Leu gcg Al a 210 at t SIle cat Hi s gcc Al a acg Thr gt g Val 290 ccg Pr o gt g Val cac Hi s gag G u gag G u 370 aat Asn ttt Phe gcg Al a tgt Cys gag G u gt g Val t ca Ser aat Asn gt g Val 260 ttc Phe at c SIle acc Thr gaa G u cgt Ar g 340 cat Hi s gcg Al a tgg Tr p ggt G y ggt G y 420 gat Asp gcg Al a aag Lys ccg Pr o ccg Pr o 245 gat Asp cct Pr o aac Asn ggt G y aaa Lys 325 aag Lys gt g Val gat Asp cat Hi s t ca Ser 405 gcg Al a ggt G y 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 ggt ttg ggc atg ctg ctg ggt gag ctt ctg acc gtt aag ctg cac caa Page 117 G y Leu G y 440 IVet Leu Leu y Gene Sequence 24- 2- 06. txt G u Leu Leu Thr Val Lys Leu His G n 445 450 ctt Leu aag Lys 470 gag G u gt a Val ttg Leu gcg Al a agc Ser 550 at c SIle ct g Leu gag G u gt g Val at c SIle t at Tyr 520 tcg Ser gcg Al a ct g Leu gct Al a ct c Leu ttc Phe 490 gat Asp gga Gy cca Pr o acc Thr cgt Ar g 570 gt g Val gt g Val 475 gca Al a ccg Pr o cct Pr o cca Pr o cga Ar g 555 tcg Ser gt g Val 460 gag G u gag G u aag Lys gt a Val acc Thr 540 acc Thr aac Asn ttt Phe gga Gy at t SIle aaa Lys ct g Leu 525 at c SIle gt c Val at a I le aac Asn cag G n gcg Al a gtt Val 510 at c SIle acg Thr ttt Phe agg Ar g aac Asn cca Pr o gcg Al a 495 cgc Ar g gat Asp tgg Tr p ggt G y aat Asn 575 agt Ser gaa G u 480 gct Al a gag G u at c lile gaa G u gga G y 560 at t SIle tct Ser 465 ttt Phe gcg Al a cag G n gt c Val cag G n 545 gga G y cct Pr o ttg Leu ggt Gy ggt Gy ct a Leu acg Thr 530 gt c Val gt a Val act Thr ggc G y act Thr at c SIle gct Al a 515 gat Asp at g Net gga G y cca Pr o at g bet gac Asp aaa Lys 500 gag G u cct Pr o gga Gy gcg Al a 1507 1555 1603 1651 1699 1747 1795 1837 1860 tgatgat t ga t acacct gct gt t <210> 86 <211> 579 <212> PRT <213> Corynebacter i um gl ut am cum <400> 86 IVet Ala His Ser Tyr Al a u G n Leu 1 5 Sy Val Lys Arg lie Tyr Gy Leu Val Val Asp Ala Val Arg n Ser Asp Ile G u G u Al a Al a Ala Phe Ala Ala y 55 G u Leu Ala Val Cys Al a Ala Ser Cys 70 lie n G y Leu Tyr Asp Ser His Arg Ile Ala Ser His Ile Pro Ser Ala n 100 105 G u Thr His Pro G u Ile Leu Phe Lys 115 120 I e Asp Thr 10 G y Asp Ser Su Trp Val Al a G u Ser G y Pro G y Asn G y Al a 90 li e G y Ser G u Cys Ser Page 118 Al a Pr o Ar g Thr Hi s Leu Phe Cys MVt Val Asn Gy Gy Gu n 130 135 Gene Sequence 24-2-06. t xt G y G u Arg lie Leu His His Ala lie G n 145 Asp SIle Al a Sy Lys 225 G n Gy Gy G n Tyr 305 Hi s Al a Val Asn Cys 385 Asp Hi s bet Val Ser 465 Phe Thr Al a Ser Val 195 Gy Lys G u Cys Asp 275 Asp Val Lys G u Lys 355 Leu Val Val SIle Gy 435 Leu Gy Thr Al a G u 165 Thr Al a Lys Pr o Pr o 245 Asp Pr o Asn Gy Lys 325 Lys Val Asp Hi s Ser 405 Al a Gy G n Val Hi s 485 Gy 150 Asp Pr o l Ie Asn SIle 230 Phe Al a Ty r Gy Asp 310 Thr Leu Pr o Lys Al a 390 Phe G n Gy Leu Lys 470 G u Lys Al a Val Asn Al a 215 Gy G u Ser Ser Al a 295 Val Asp Ser SIle Asp 375 Ar g Ar g Ser Leu Pr o 455 Leu G u Val Asp Phe 185 Al a Al a Al a Gy G u 265 Phe l Ie Al a Ser Val 345 Pr o Val SIle Gy Asp 425 et Lys Aet Asn Ser Gy 170 Pr o Lys G n Leu bet 250 Al a Leu Sy Thr Phe 330 Val G u Phe G u Thr 410 Ar g Leu Al a Leu Val 155 Thr Asp Ser Val Sy 235 Ser Asp Pr o Ar g SIle 315 Leu G u Tyr Thr Asn 395 vet Asn Leu Val Val 475 Val Ser Thr Thr 205 G u Lys Leu Leu Asp 285 Thr Asn Ar g Tyr Al a 365 Asp G u Asn G n G u 445 Phe Sy Pr o Ser 175 Al a Phe Al a Tyr Sy 255 Leu Val Val Leu Leu 335 Hi s SIe Sy Thr Leu 415 I e Leu Asn Pr o Phe Ala u Ile Ala Ala Ala Page 119 Al a G n Val G n 545 Gy Pr o lie Lys Ser Val Arg 500 Ala G u Ala Leu Al a 515 Asp Pro Asn Ala Leu 535 Met G y Phe Ser Lys 550 Sy Ala IVet lle Asp 565 Pr o Gene Sequence 24-2-06.txt Ile Thr Asp Pro Lys Lys Val Arg G u 505 510 Tyr Pro y Pro Val Leu Ile Asp Ile 520 525 Ser lie Pro Pro Thr Ile Thr Trp G u 540 Ala Ala Thr Arg Thr Val Phe Gy y 555 560 Leu Ala Arg Ser Asn lie Arg Asn Ile 570 575 <210> 87 <211> 552 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (88)..(552) <223> FRXA02807 <400> 87 aaagcgtggc aacaactgga atttaagagc acaattgaag tcgcaccaag ttaggcaaca caatagccat aacgt t gagg agt t cag atg gca cac agc tac gca gaa caa tta IVet Al a Hi s Ser Tyr Al a G u G n Leu 1 at t I e ggt Gy gag Su gcg Al a ggt Sy aat Asn at t SIle gaa G u act Thr agc Ser gt g Val tcg Ser gga G y gcg Al a tcg Ser tct Ser gaa G u aat Asn gtt Val at c SIle aca Thr gt g Val ttc Phe 110 t ac Tyr gct Al a 15 ccg Pr o cga Ar g act Thr cac Hi s tt g Leu 95 tt c Phe tgc Cys caa G n at c SIle aat Asn ggg G y ct g Leu gcc Al a cag G n gag G u ggt Gy gt g Val gag G u gag G u 65 at t SIle at c l Ie gaa G u at g Nbet gt g Val gat Asp gaa ct g Leu cag G n gct Al a acg Thr gt g Val 130 aag Lys gct Al a 35 gcg Al a gca Al a ggt G y agc Ser cat Hi s 115 aat Asn cga Ar g gt c Val gcg Al a gt a Val ctt Leu cat Hi s 100 ccg Pr o ggt Gy t at Tyr caa G n ttt Phe gct Al a gat Asp ccg Pr o at t SIle gag G u ggt G y t ca Ser gca Al a gct Al a tcg Ser agt Ser ttg Leu cag G n 135 ttg Leu gat Asp gcc Al a tct Ser cat Hi s gcc Al a ttt Phe 120 ggt Gy 114 162 210 258 306 354 402 450 498 Page 120 Gene Sequence 24- 2- 06. txt cgc att ttg cat cac gcg att cag tcc acc atg gcg ggt aaa ggt gtg 546 Arg lie Leu His His Ala lie On Ser Thr IVet Ala G y Lys y Val 140 145 150 tcg gtg 552 Ser Val 155 <210> 88 <211> 155 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 88 IVet Ala His Ser Tyr Ala Gu n Leu Ile Asp Thr Leu u Ala n 1 5 10 Sy Val Lys Arg lie Tyr y Leu Val y Asp Ser Leu Asn Pro lie 25 Val Asp Ala Val Arg n Ser Asp Ile G u Trp Val His Val Arg Asn 40 Gu Gu Al a Ala Ala Phe Ala Ala G y Ala Gu Ser Leu Ile Thr G y 55 G u Leu Ala Val Cys Al a Ala Ser Cys G y Pro G y Asn Thr His Leu 70 75 lie G n y Leu Tyr Asp Ser His Arg Asn G y Ala Lys Val Leu Ala 90 IIe Ala Ser His IIe Pro Ser Ala G n IIe G y Ser Thr Phe Phe G n 100 105 110 G u Thr His Pro G u IIe Leu Phe Lys G u Cys Ser G y Tyr Cys G u 115 120 125 Vet Val Asn G y G y G u n G y G u Arg IIe Leu His His Ala Ile 130 135 140 G n Ser Thr Net Ala G y Lys y Val Ser Val 145 150 155 <210> 89 <211> 944 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> <223> FRXA00635 <400> 89 ggt acg gat ttc cct tat tct gat ttc ctt cct aaa gac aac gtt gcc 48 Sy Thr Asp Phe Pro Tyr Ser Asp Phe Leu Pro Lys Asp Asn Val Ala 1 5 10 cag gtg gat atc aac ggt gcg cac att ggt cga cgt acc acg gtg aag 96 G n Val Asp IIe Asn G y Ala His I I e G y Arg Arg Thr Thr Val Lys 25 tat ccg gtg acc ggt gat gtt gct gca aca atc gaa aat att ttg cct 144 Tyr Pro Val Thr y Asp Val Ala Ala Thr I e u Asn Ile Leu Pro Page 121 Gene Sequence 24-2-06.txt cat Hi s gca Al a gt c Val aac Asn tgc Cys gac Asp cat Hi s 145 at g let gtt Val tct Ser ttt Phe gcg Al a 225 cag G n gt c Val cag G n gga Gy aag Lys gag G u aag Lys ct g Leu gt g Val 115 gt g Val at t SIle ggc Gy ct g Leu ggc Gy 195 act Thr at c l Ie gct Al a gat Asp at g et 275 gga Gy aaa Lys aag Lys gt g Val gat Asp cat Hi s t ca Ser gcg Al a ggt Gy 165 caa G n gt g Val cat Hi s tcg Ser gca Al a 245 aat Asn ttc Phe at g let aca gat Thr Asp ttg agc Leu Ser 70 cct at t Pro Ile aag gat Lys Asp gcg agg Al a Ar g ttc cgc Phe Arg 135 caa agt G n Ser 150 ggt ttg G y Leu ctt ccg Leu Pro aag ttg Lys Leu gag gaa G u G u 215 gt a cgc Val Arg 230 ttg gca Leu Al a gcg ctg Al a Leu agc aag Ser Lys at c gat Ile Asp 295 cgt t cc Arg Ser tcg gtg Ser Val cac cct His Pr o gcg gtg Al a Val 105 tac at c Tyr Ile 120 cac ggc Hi s G y gtt gat Val Asp ggc atg G y let ctg aag Leu Lys 185 gag atg Su lbet 200 gtg aat Val Asn atc acc Ile Thr tat cct Tyr Pro tcg at c Ser Ile 265 gcg gcc Al a Al a 280 ctg gcc Leu Al a ttc ctt Phe Leu gta gag Val G u 75 gaa t ac G u Tyr 90 ttt act Phe Thr gag aat Su Asn acg at g Thr lbet cga aac Arg Asn 155 ctg ct g Leu Leu 170 gct gtg Al a Val ctc gt g Leu Val ttc gca Phe Al a gat ccg Asp Pro 235 gga cct G y Pro 250 cca cca Pro Pro acc cga Thr Arg cgt tcg Arg Ser cgg Ar g t ac Tyr gcc Al a gat Asp gag G u 125 aat Asn cag G n gag G u ttt Phe gga Gy 205 at t l Ie aaa Lys ct g Leu at c SIle gt c Val 285 at a SIle at g Net aca Thr tct Ser acc Thr 110 gga Gy gcg Al a gt g Val ctt Leu aac Asn 190 cag G n gcg Al a gtt Val at c SIle acg Thr 270 ttt Phe agg Ar g ct c Leu cat Hi s at t SIle ggc Gy acg Thr ttg Leu at c SIle ct g Leu 175 aac Asn cca Pr o gcg Al a cgc Ar g gat Asp 255 tgg Tr p ggt Gy aat Asn aag Lys aac Asn ttg Leu at g let cgc Ar g cct Pr o gcg Al a 160 acc Thr agt Ser gaa G u gct Al a gag G u 240 at c SIe gaa G u gga Gy att SIe cct act cca Pro Thr Pro tgatgat t ga t acacct gct gtt Page 122 Gene Sequence 24-2-06.txt 305 <210> <211> 307 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> Gy 1 G n Tyr Hi s Al a Val Asn Cys Asp Hi s 145 et Val Ser Phe Al a 225 G n Val G n Gy Pr o 305 Thr Asp Phe Val Asp II e Pro Val Thr Val Lys G u His G u Ar g G u Lys Hi s G u Leu Al a 100 Asn Val Trp 115 Phe Val G y 130 Al a I e G y Cys G y Asp Lys Leu Hi s 180 Leu G y IVet 195 G y Thr Asp 210 G y Ile Lys Leu Al a G u Thr Asp Pro 260 Val [et G y 275 Val Gy Al a 290 Thr Pro Pr o 5 Asn Gy Lys Lys Val Asp Hi s Ser Al a Sy 165 G n Val Hi s Ser Al a 245 Asn Phe vet Tyr Ser Asp Phe Leu 10 Sy Ala His lie G y Asp Val Ala Ala Thr 40 Thr Asp Arg Ser Phe Leu Ser Ser Val Val 70 Pro lie His Pro u 90 Lys Asp Ala Val Phe 105 Al a Arg Tyr Ile G u 120 Phe Arg His G y Thr 135 G n Ser Val Asp Arg 150 G y Leu G y IVet Leu 170 Leu Pro Leu Lys Ala 185 Lys Leu G u IVet Leu 200 G u G u Val Asn Phe 215 Val Arg Ile Thr Asp 230 Leu Ala Tyr Pro y 250 Ala Leu Ser Ile Pro 265 Ser Lys Ala Ala Thr 280 Ile Asp Leu Ala Arg 295 Pr o Ar g SIle Leu G u 75 Tyr Thr Asn vet Asn 155 Leu Val Val Al a Pr o 235 Pr o Pr o Ar g Ser Asp Thr Asn Ar g Tyr Al a Asp G u 125 Asn G n G u Phe Sy 205 l le Lys Leu SIle Val 285 I le Val Val Leu Leu Hi s l Ie Sy Thr Leu SIle Leu 175 Asn Pr o Al a Ar g Asp 255 Tr p Sy Asn Al a Lys Pr o Lys Asn Leu bet Ar g Pr o Al a 160 Thr Ser G u Al a G u 240 I e G u Gy lie Page 123 ene Sequence 24- 2- 06. txt <210O> 91 <21 1> 954 <212> DNA <213> COr ynebact er i urn gi ut arr cumn <220> <221 ODS <222> (931) <223> RXN03044 <400> 91 tacgccaccc acggcaaggc catgattccg ctgtacatct ccagcgcacc ggt gact cca tct gggcagc agccgat cag t ct act cgat gt tcgggat t at g gca cgt ggc t tc 11! [Vb t Ala Arg G31y Ph e ct c Le u c ag O31n gt c ValI c ac Hi s at c I Ie cca Pr o t cc Ser gt t ValI t ac Tyr gct A a 1 50 gca A a ggc G31y ggc G31y at g vb t ac c Thr ggc G31y t ac Tyr gga G31y ggt G31y at g vb t 120 gt t ValI g at As p gct A a t ac Tyr ac c Thr gga G31y g ac As p g ac As p ac c Thr g ac As p ggc G31y t gg Tr p gcc A a gct A a gag O31u 1 70 gca A a gca A a cac Hi s cca Pr o cgc Ar g at c I Ie 75 gt a ValI acc Thr gct A a aac As n gct A a 155 gca A a gt g ValI ggt G31y t cc Ser t cc Ser at g vb t t ac Tyr g aa O31u G31y ct c Le u at t I Ie 140 cgt Ar g t tc Ph e t ct Ser ac c Thr gt c ValI gcg A a ggc G31y gag O31u ct g Le u gag O31u 110 gct A a t cc Ser aag Ly s ac c Thr t tc Ph e 1 90 ac c Thr 15 tt g Le u t ac Tyr cca Pr o cca Pr a c ac Hi s 95 gca A a gca A a gct A a gca A a ac c Thr 1 75 t cc Ser ct g Le u gct A a gag O31u G31y ac c Thr aag Ly s aac As n t cc Ser act Thr c ag O31n 1 c ag O31n act Thr 1 ac c Thr t cc Ser at c I Ie aag Ly s cca Pr a G31y at c I Ie at c I Ie t ct Ser 145 ct g Le u ct g Le u g at As p ggt G31y ac c Thr gca A a ggt G31y c ag O31n at c I Ie tt g Le u ct t Le u 130 t gg Tr p cgc Ar g aag Ly s ct g Le u g aa O31u aac As n c ac Hi s g aa O31u cca Pr a t ac Tyr gct A a 115 gag O31u gt t ValI aac As n c ag O31n cca Pr a 1 ggc O31y gag O31u ct g Le u g at As p gct A a ct c Le u 1 00 t cc Ser gct A a aac As n cca Pr a ac c Th r 1 aac As n at c cgt gaa t gg gt c cca ggc gac t ac acc gt t ct c ggt gca gat ggc Page 124 Ile Arg Gu Trp Val Pro y 200 ttc ggt ttc tct gat acc cgc Phe Gy Phe Ser Asp Thr Arg 215 220 gac gct gag tcc att gtt gtt Asp Ala u Ser lie Val Val 230 235 ggc aag atc gac gtc tcc gtt Sy Lys Ile Asp Val Ser Val 250 ttg gat gat cct acg agt gtt Leu Asp Asp Pro Thr Ser Val 265 taaatcacct caagggacag ata <210> 92 <211> 277 <212> PRT <213> Corynebacter i um gl ut ar Gene Sequence 24-2-06.txt Asp Tyr Thr Val Leu Gy Al a 205 210 cca gct gct cgt cgc ttc ttc Pro Ala Ala Arg Arg Phe Phe 225 gca gtg ctg aac tcc ctg gca Ala Val Leu Asn Ser Leu Ala 240 gct gct cag gct gct gag aag Ala Ala G n Ala Ala G u Lys 255 t cc gta gat cca aac gct cct Ser Val Asp Pro Asn Ala Pro 270 275 Asp aac Asn cgc Ar g ttc Phe 260 gag Gu <400> 92 [et Al a 1 Thr G y Ser Thr I e Al a Lys G y Pro G n Sy l ie I e Leu I e Leu 130 Ser Trp 145 Leu Arg Leu Lys Asp Leu Arg y Phe Leu G u Asn Hi s G(3 u Pr o Tyr Al a 115 G u Val Asn G n Pr o 195 5 Leu Sy Val Val G u Tyr Sy Asp Leu Sy 165 Ser G n G n Val Hi s SIle 70 Pr o Ser Val Tyr Al a 150 Al a Gy I e Leu Hi s G u Ar g Tyr G u Ar g Sy Sy 135 Ar g Asp Pr o Ar g Sy bet Thr 40 Sy Tyr Sy Sy et 120 Val Asp Al a Tyr G u 200 Al a Asp Tyr SIle SIle Leu G u 105 G n Ar g Sy Sy Val 185 Tr p Thr 10 Sy Asp Asp Thr Asp 90 Sy Tr p Al a Al a G u 170 Al a Val Al a Hi s Pr o Ar g SIle Val Thr Al a Asn Al a 155 Al a Val Pr o Ar g Pr o Phe Tyr Asn Sy Hi s Lys 125 Tyr Asn Val Asp Asp 205 Thr Val Al a Sy G u Leu G u 110 Al a Ser Lys Thr Phe 190 Tyr Thr Leu Tyr Pr o Pr o Hi s Al a Al a Al a Al a Thr 175 Ser Thr Leu y Ala Asp y Phe y Phe Ser Asp Thr Arg Pro Ala Ala Arg Page 125 Gene Sequence 24-2-06.txt 210 215 220 Arg Phe Phe Asn Ile Asp Ala u Ser lie Val Val Ala Val Leu Asn 225 230 235 240 Ser Leu Al a Arg G u G y Lys Ile Asp Val Ser Val Al a Al a n Al a 245 250 255 Ala G u Lys Phe Lys Leu Asp Asp Pro Thr Ser Val Ser Val Asp Pro 260 265 270 Asn Ala Pro G u G u 275 <210> 93 <211> 302 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> FRXA02852 <400> 93 gtt gca gtg tct gac ttc tcc act gat ctg cca aac cag atc cgt gaa 48 Val Ala Val Ser Asp Phe Ser Thr Asp Leu Pro Asn n lie Arg G u 1 5 10 tgg gtc cca ggc gac tac acc gtt ctc ggt gca gat ggc ttc ggt ttc 96 Trp Val Pro y Asp Tyr Thr Val Leu y Ala Asp y Phe y Phe 25 tct gat acc cgc cca gct gct cgt cgc ttc ttc aac atc gac gct gag 144 Ser Asp Thr Arg Pro Al a Ala Arg Arg Phe Phe Asn Ile Asp Ala G u 40 tcc att gtt gtt gca gtg ctg aac tcc ctg gca cgc gaa ggc aag atc 192 Ser lie Val Val Ala Val Leu Asn Ser Leu Ala Arg G u y Lys Ile 55 gac gtc tcc gtt gct gct cag gct gct gag aag ttc aag ttg gat gat 240 Asp Val Ser Val Ala Al a G n Ala Ala G u Lys Phe Lys Leu Asp Asp 70 75 cct acg agt gtt tcc gta gat cca aac gct cct gag gaa taaatcacct 289 Pro Thr Ser Val Ser Val Asp Pro Asn Ala Pro G u G u caagggacag ata 302 <210> 94 <211> 93 <212> PRT <213> Cor ynebact er i um gl ut ari cum <400> 94 Val Ala Val Ser Asp Phe Ser Thr Asp Leu Pro Asn n lie Arg G u 1 5 10 Trp Val Pro y Asp Tyr Thr Val Leu y Ala Asp y Phe y Phe 25 Ser Asp Thr Arg Pro Al a Ala Arg Arg Phe Phe Asn Ile Asp Ala G u 40 Page 126 ene Sequence 24- 2- 06. txt Ser lieVal Val Ala Val Leu Asn Ser Leu Ala Arg Ou Gy Lys Ilie 55 Asp Val Ser Val Ala Ala On Ala Ala O31u Lys Phe Lys Leu Asp Asp 70 75 Pr o Thr Ser Val Ser Val Asp Pro Asn Ala Pro O31u O31u <210O> <21 1> 954 <212> DNA <213> Cor ynebact er i urn gI ut anri cumn <220> <221 ODS <222> (931) <223> FRXA00268 <400> tacgccaccc acggcaaggc catgattccg ctgtacatct ccagcgcacc ggtgactcca tttgggcagc agccgatcag t ct act cgat gt tcgggat t tt t Ph e c ag O31n gt c ValI c ac Hi s at c I Ie cca Pr o t cc Ser gt t ValI t ac Tyr gct Al a 1 50 ggc G31y at g vb t ac c Thr ggc G31y t ac Tyr gga G31y ggt G31y at g vb t 120 gt t ValI g at As p ac c Thr gga G31y g ac As p g ac As p ac c Thr g ac As p ggc G31y t gg Tr p gcc Al a gct Al a gca Al a cac Hi s cca Pr o cgc Ar g at c I e 75 gt a ValI acc Thr gct Al a aac As n gct Al a 155 ggt G31y t cc Ser t cc Ser at g vb t t ac Tyr g aa O31u G31y ct c Le u at t I Ie 140 cgt Ar g ac c Thr gt t ValI gcg Al a ggc G31y gag O31u ct g Le u gag O31u 110 gct Al a t cc Ser aag Ly s ac c Thr 15 tt g Le u t ac Tyr cca Pr o cca Pr o c ac Hi s 95 gca Al a gca Al a gct Al a gca Al a tt g Le u gct Al a gag O31u G31y ac c Thr 80 aag Ly s aac As n t cc Ser act Thr c ag O31n 1 60 at g gca cgt I'b t Al a Ar g 1 acc ggt gaa Thr 03 y 03 u t cc acc aac Ser Thr Asn at c gca cac Ile Al a Hi s aag ggt gaa Lys 03 y 03 u cca cag cca Pro O31n Pr o ggc at c t ac O3 y Il e Tyr at c t tg gct Il e Leu Al a 115 at c ct t gag Il e Leu 03 u 130 t ct t gg gt t Ser Tr p Val 145 ct g cgc aac Leu Arg Asn ggc tt c O3 y Phe ggc ct c O3 y Leu gag ggt O31u (1y ct g gt t Leu Val gat gt t As p Val gct gag Al a 03 u ct c t ac Leu Tyr 1 00 t cc ggt Ser 03 y gct gac Al a As p aac tt g Asn Leu cca ggt Pro O31y 1 gca gat gct ggc gag gca ttc gta acc acc cag ctg aag cag acc tcc Page 127 Ala Asp Ala Gy G u Al a Phe 170 ggc cca tac gtt gca gtg tct Gy Pro Tyr Val Ala Val Ser 185 atc cgt gaa tgg gtc cca ggc Ile Arg G u Trp Val Pro G y 200 ttc ggt ttc tct gat acc cgc Phe y Phe Ser Asp Thr Arg 215 220 gac gct gag tcc att gtt gtt Asp Ala u Ser lie Val Val 230 235 ggc aag atc gac gtc tcc gtt Sy Lys Ile Asp Val Ser Val 250 ttg gat gat cct acg agt gtt Leu Asp Asp Pro Thr Ser Val 265 taaatcacct caagggacag ata <210> 96 <211> 277 <212> PRT <213> Cor ynebact er i um gl ut ar Gene Sequence 24-2-06.txt Val Thr Thr G n Leu Lys G n 175 gac ttc tcc act gat ctg cca Asp Phe Ser Thr Asp Leu Pro 190 195 gac tac acc gtt ctc ggt gca Asp Tyr Thr Val Leu y Al a 205 210 cca gct gct cgt cgc ttc ttc Pro Ala Ala Arg Arg Phe Phe 225 gca gtg ctg aac tcc ctg gca Ala Val Leu Asn Ser Leu Ala 240 gct gct cag gct gct gag aag Ala Ala G n Ala Ala G u Lys 255 t cc gta gat cca aac gct cct Ser Val Asp Pro Asn Ala Pro 270 275 Thr 180 aac Asn gat Asp aac Asn cgc Ar g ttc Phe 260 gag G u Thr Leu Tyr Pr o Pr o Hi s Al a Al a Al a Al a <400> 96 [et Al a 1 Thr G y Ser Thr I e Al a Lys G y Pro G n Sy l ie I e Leu I e Leu 130 Ser Trp 145 Leu Arg Arg G y (3 u G y Asn u Hi s Leu G u Asp Pro Al a Tyr Leu 100 Al a Ser 115 G u Al a Val Asn Asn Pro Phe 5 Leu Gy Val Val G u Tyr Gy Asp Leu Gy 165 Phe G n Val Hi s SIle 70 Pr o Ser Val Tyr Al a 150 Al a Leu Hi s G u Ar g Tyr G u Ar g Sy Sy 135 Ar g Asp Al a Asp 25 Tyr SIle SIle Leu G u 105 G n Ar g Sy Gy Thr 10 Sy Asp Asp Thr Asp 90 Sy Tr p Al a Al a Al a Hi s Pr o Ar g SIle Val Thr Al a Asn Al a 155 Ar g Pr o Phe Tyr Asn Sy Hi s Lys 125 Tyr Asn Thr Val Al a Sy G u Leu G u 110 Al a Ser Lys G u Ala Phe Val Thr Thr G n 170 175 Page 128 Leu Asp Leu Ar g 225 Ser Al a Asn G n Pr o 195 Al a Phe Al a Lys Pr o 275 Ser G n Gy SIle G u 245 Lys G u Pr o Ar g Gy 215 Al a Lys Asp Gene Sequence 24-2-06.txt Tyr Val Ala Val Ser Asp Phe 185 190 G u Trp Val Pro y Asp Tyr 200 205 Phe Ser Asp Thr Arg Pro Ala 220 Su Ser lie Val Val Ala Val 235 Ile Asp Val Ser Val Ala Ala 250 Asp Pro Thr Ser Val Ser Val 265 270 Thr Val Ar g Asn 240 Al a Pr o <210> 97 <211> 508 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(508) <223> RXN03086 <400> 97 ttcgtgcact tcggcgtgtc acaattaggt acgaccaaga cgtataaacg aaataaaaca ttccaacagg aggtgtggaa atgggaccgg gaaaccggga gcc gat caa Al a Asp G n aaa Lys gat Asp gag G u cgt Ar g cgc Ar g cca Pr o cgt Ar g ggt Gy gt g Val at g Net cgt Ar g tct Ser tct Ser cgt Ar g aag Lys tct Ser t ca Ser ct c Leu ccc Pr o gaa G u tgg Tr p tcg Ser ttg Leu gac Asp ctt Leu at g Net gaa G u cgc Ar g gac Asp gac Asp tta Leu ttg Leu t ca Ser cca Pr o aac Asn 110 tct Ser 15 t ca Ser ct c Leu ctt Leu acc Thr ggc Gy 95 gca Al a aac Asn gat Asp cag G n gag G u gac Asp 80 gat Asp gcc Al a gcg Al a gag G u tct Ser gca Al a gt c Val gaa G u at g bet at c I e acc Thr cca Pr o gca Al a acc Thr gag G u 100 cac Hi s gca Al a cgc Ar g aac Asn gaa G u aag Lys att SIe aag Lys cgc Ar g Page 129 Gene Sequence 24- 2- 06. txt gct cag cga cca ggc atc ggc gtc ggc gga cac att tcc act tac gca 499 Al a n Arg Pro G y Ile y Val G y Gy His Ile Ser Thr Tyr Ala 120 125 130 ggc gca gcc 508 G y Al a Al a 135 <210> 98 <211> 136 <212> PRT <213> Corynebacter i um gl utam cum <400> 98 IVet Ala Asp G n Ala Lys Leu Gy y Lys Pro Ser Asp Asp Ser Asn 1 5 10 Phe Ala IVet lie Arg Asp y Val Ala Ser Tyr Leu Asn Asp Ser Asp 25 Pro G u G u Thr Asn G u Trp IVet Asp Ser Leu Asp y Leu Leu G n 40 Su Ser Ser Pro G u Arg Ala Arg Tyr Leu IVet Leu Arg Leu Leu G u 55 Arg Ala Ser Ala Lys Arg Val Ser Leu Pro Pro IVet Thr Ser Thr Asp 70 75 Tyr Val Asn Thr Ile Pro Thr Ser Iet G u Pro G u Phe Pro y Asp 90 G u G u Iet G u Lys Arg Tyr Arg Arg Trp lie Arg Trp Asn Ala Ala 100 105 110 Ile Vet Val His Arg Al a n Arg Pro G y Ile G y Val G y G y His 115 120 125 Ile Ser Thr Tyr Al a y Al a Al a 130 135 <210> 99 <211> 508 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(508) <223> FRXA02887 <400> 99 ttcgtgcact tcggcgtgcc acaattaggt acgaccaaga atgggaccgg gaaaccggga cgtataaacg aaataaaaca ttccaacagg aggtgtggaa atg gcc gat caa gca 115 IVet Ala Asp G n Ala 1 aaa ctt ggt ggc aag ccc tcg gat gac tct aac ttc gcg atg atc cgc 163 Lys Leu G y G y Lys Pro Ser Asp Asp Ser Asn Phe Al a IVet Ile Arg 15 gat ggc gtg gca tct tat ttg aac gac tca gat ccg gag gag acc aac 211 Asp y Val Ala Ser Tyr Leu Asn Asp Ser Asp Pro G u G u Thr Asn 30 Page 130 ene Sec gag tgg at g gat t ca ct c gac gga tt a O31u Tr p I'b t Asp Ser Leu Asp G31y Leu 45 cgt gct cgt t ac ct c at g ct t cgt tt g Ar g Al a Ar g Ty r Leu kt Leu Ar g Leu 60 cgc gt a t ct ct t ccc cca at g acg t ca Ar g Val Ser Leu Pr o Pr o I'b t Thr Ser 75 cca acc t ct at g gaa cct gaa t tc cca Pr o Thr Ser 'b t O31u Pro O31u Phe Pr o cgt t ac cgt cgt t gg at t cgc t gg aac Ar g Ty r Ar g Ar g Tr p I Ie Ar g Tr p As n 105 110 gct cag cga cca ggc at c ggc gt c ggc Ala O31n Ar g Pr o 31y Ilie 31y Val G31y 120 125 ggc gca gcc G y Al a Al a 135 <210> 100 <211> 136 <212> PRTF <213> Cor ynebact er i urn gi ut ani curn <400> 100 I'b t Al a Asp n Al a Lys Leu 0 y 0 y 1 5 Phe Al a I'b t lieArg Asp Gy Val Al a Pro 03 u 03 u Thr As n 03 u Tr p I'bt Asp 40 031u Se r Ser Pr o 031u Ar g Al a Ar g Tyr Ar g Al a Ser Al a Lys Ar g Val Ser Leu 70 Tyr Val Asn Thr I Ie Pro Thr Ser I'b t 031u 031u 'b t 031u Lys Ar g Tyr Ar g Ar g 100 105 Ilie I'b t Val Hi s Ar g Al a O31n Ar g Pr o 115 120 Il e Ser Thr Tyr Al a 03 y Al a Al a 130 135 <210> 101 <211> 1385 <212> DNA <21 3> Cor ynebact er i urn gi ut anm curn iuence 24-2- 06. txt ctc cag gag t ct t ct Leu 03 n 03 u Ser Ser ct t gag cgt gca t ct Leu G31u Ar g Al a Ser acc gac t ac gt c aac Thr Asp Tyr Val Asn 80 ggc gat gag gaa at g 31y Asp (1u (1u Ib t 95 gca gcc atc atg gtt Al a Al a IlIe I'b t Val 115 gga cac at t t cc act O3 y Hi s Il e Ser Thr 130 cca Pr o gca Al a ac c Thr gag O31u 1 00 c ac Hi s t ac Tyr g aa O31u aag Ly s at t lIe aag cgc Ar g gca Al a Pr o Tyr Le u vb t Pr o Pr o I Ie I Ie As p As n G31y Ar g Thr Ph e Tr p ValI 125 Ser Ser Le u Le u Th r G31y Al a (31y Page 131 Gene Sequence 24-2-06.txt <220> <221> CDS <222> (1)..(1362) <223> RXN03043 <400> 101 gat Asp 1 ccg Pr o act Thr ttc Phe cac Hi s cgt Ar g ttc Phe aac Asn tgg Tr p gag G u 145 gat Asp gag G u at g Met cgc Ar g cca Pr o 225 aac Asn gat Asp t ac Tyr t cc Ser cgc Ar g tgg Tr p ct c Leu gtt Val aag Lys 115 gt g Val gac Asp gac Asp ttc Phe gac Asp 195 gtt Val gt c Val gaa G u ttc Phe 5 cac Hi s ggt Gy ct c Leu ttc Phe cag G n tgc Cys at c SIle aag Lys gat Asp cag G n 165 gga Gy gaa G u gca Al a ctt Leu cgt Ar g cgt cag gaa gtt tcc cgt gag cag ggt Arg n G u Val Ser Arg G u G n G y in cca Pr o ctt Leu gaa G u ctt Leu 70 cag G n aac Asn cag G n gtt Val ggt G y 150 acc Thr cgt Ar g at c SIle gcc Al a gct Al a 230 aac Asn cac Hi s ggc Sy aac Asn ggc Sy gct Al a ct g Leu gaa G u gtt Val 135 gca Al a ttc Phe gac Asp tgg Tr p t ac Tyr 215 cac Hi s gca Al a ggt Sy cca Pr o 40 cgt Ar g gac Asp gca Al a cag G n ct c Leu 120 tgg Tr p ctt Leu aag Lys cca Pr o aag Lys 200 aag Lys acc Thr acc Thr at g let at g let ggc Sy ggc Sy ct g Leu cgt Ar g 105 gag G u ggt G y gtt Val gct Al a cgc Ar g 185 ctt Leu cga Ar g at t SIle cac Hi s aag Lys gat Asp at c SIle gaa G u aac Asn 90 ct c Leu t cc Ser cgc Ar g gag G u aac Asn 170 acc Thr cca Pr o gct Al a aag Lys gac Asp gcc Al a aag Lys at g et 75 aac Asn gac Asp ttc Phe gag G u at c lile 155 gac Asp gca Al a cgt Ar g ctt Leu ggc Sy 235 ttc Phe at t l Ie gac Asp gac Asp ct g Leu gga G y ttc Phe tgg Tr p 140 at g bet ggc Sy aag Lys ggc Sy gag G u 220 t ac Tyr tgg Tr p t ac Tyr acc Thr gag G u gac Asp cct Pr o cgt Ar g 125 gat Asp aac Asn gca Al a ct c Leu ggc Sy 205 acc Thr gga Sy ggc att Sy Ile ttc cca Phe Pro gca cgt Al a Ar g gac cag Asp n gaa t ca Su Ser ctg acc Leu Thr cgc ggt Arg G y gca ggc Al a y ctt ctg Leu Leu acc tcc Thr Ser 160 gtt cgt Val Arg 175 gag aac Su Asn gat tac Asp Tyr gat cgc Asp Arg ggc cac G y Hi s 240 cag atg aag aag ctg acg ctt G n IVet Lys Lys Leu Thr Leu Page 132 Gene Sequence 24-2-06.txt 245 250 255 gat gat ctg aag ttg ttc cgc gac aag cag ggc atc cca atc acc gat 816 Asp Asp Leu Lys Leu Phe Arg Asp Lys n Gy lie Pro lie Thr Asp 260 265 270 gag cag ctg gag aag gat cct tac ctt cct cct tac tac cac cca ggt 864 G u G n Leu G u Lys Asp Pro Tyr Leu Pro Pro Tyr Tyr His Pro G y 275 280 285 gaa gac gct cct gaa atc aag tac atg aag gaa cgt cgc gca gcg ctc 912 G u Asp Ala Pro G u Ile Lys Tyr IVet Lys G u Arg Arg Ala Ala Leu 290 295 300 ggt ggc tac ctg cca gag cgt cgt gag aac tac gat cca att cag gtt 960 Sy y Tyr Leu Pro u Arg Arg u Asn Tyr Asp Pro lie n Val 305 310 315 320 cca cca ctg gat aag ctt cgc tct gtc cgt aag ggc tcc ggc aag cag 1008 Pro Pro Leu Asp Lys Leu Arg Ser Val Arg Lys y Ser y Lys G n 325 330 335 cag atc gct acc acc atg gcg act gtt cgt acc ttc aag gaa ctg atg 1056 Sn Ile Ala Thr Thr et Ala Thr Val Arg Thr Phe Lys G u Leu bet 340 345 350 cgc gat aag ggc ttg gct gat cgc ctt gtc cca atc att cct gat gag 1104 Arg Asp Lys y Leu Al a Asp Arg Leu Val Pro Ile Ile Pro Asp G u 355 360 365 gca cgt acc ttc ggt ctt gac tct tgg ttc cca acc ttg aag atc tac 1152 Ala Arg Thr Phe y Leu Asp Ser Trp Phe Pro Thr Leu Lys Ile Tyr 370 375 380 aac ccg cac ggt cag aac tac gtg cct gtt gac cac gac ctg atg ctc 1200 Asn Pro His G y G n Asn Tyr Val Pro Val Asp His Asp Leu IVet Leu 385 390 395 400 tcc tac cgt gag gca cct gaa gga cag atc ctg cac gaa ggc atc aac 1248 Ser Tyr Arg G u Ala Pro G u G y n lie Leu His G u G y lie Asn 405 410 415 gag gct ggt tcc gtg gca tcg ttc atc gct gcg ggt acc tcc tac gcc 1296 G u Ala y Ser Val Al a Ser Phe Ile Ala Ala y Thr Ser Tyr Ala 420 425 430 acc cac ggc aag gcc atg att ccg ctg tac atc ttc tac tcg atg ttc 1344 Thr His G y Lys Ala IVet IIe Pro Leu Tyr lie Phe Tyr Ser IVet Phe 435 440 445 ggg att cca gcg cac cgg tgactccatc tgggcagcag ccg 1385 G y lie Pro Ala His Arg 450 <210> 102 <211> 454 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 102 Asp Leu Asp y Phe Arg n u Val Ser Arg Gu Gn Gy y ley 1 5 10 Pro Ser Tyr Pro His Pro His y IVet Lys Asp Phe Trp G u Phe Pro 25 Thr Val Ser IVet y Leu y Pro IVet Asp Ala Ile Tyr n Ala Arg Page 133 Gene Sequence 24-2-06.txt Arg Gy lie Lys Thr Ser Asp G n Phe Hi s Ar g Phe Asn Tr p G u 145 Asp G u vet Ar g Pr o 225 Asn Asp G u G u Gy 305 Pr o G n Ar g Al a Asn 385 Ar g Tr p Leu Val Lys 115 Val Asp Asp Phe Asp 195 Val Val G u Leu Leu 275 Al a Tyr Leu Al a Lys 355 Thr Hi s Leu Phe G n Cys SIle Lys Asp G n 165 Gy G u Al a Leu Ar g 245 Leu Lys G u Pr o Lys 325 Thr Leu Gy G n G u Leu 70 G n Asn G n Val Sy 150 Thr Ar g SIle Al a Al a 230 Asn Phe Asp SIle G u 310 Leu bet Al a Leu Asn 390 Asn Gy Al a Leu G u Val 135 Al a Phe Asp Tr p Tyr 215 Hi s Al a Ar g Pr o Lys 295 Ar g Ar g Al a Asp Asp 375 Tyr Asp Al a G n Leu 120 Tr p Leu Lys Pr o Lys 200 Lys Thr Thr Asp Tyr 280 Tyr Ar g Ser Thr Ar g 360 Ser Val Gy Leu Ar g 105 G u Sy Val Al a Ar g 185 Leu Ar g SIle Hi s Lys 265 Leu vet G u Val Val 345 Leu Tr p Pr o G u Net Asn Asn 90 Leu Asp Ser Phe Arg G u G u lie 155 Asn Asp 170 Thr Al a Pro Arg Al a Leu Lys G y 235 Sn lbet 250 G n y Pro Pro Lys G u Asn Tyr 315 Arg Lys 330 Arg Thr Val Pr o Phe Pro Val Asp 395 G u Asp Pr o Ar g 125 Asp Asn Al a Leu Sy 205 Thr Sy Lys Pr o Tyr 285 Ar g Pr o Ser Lys SIle 365 Leu Pr o Asn Val 110 Sy G u Asn Tyr Val 190 Hi s Lys Leu Leu SIle 270 Hi s Al a SIle Sy G u 350 Pr o Lys u Leu Ar g Al a Leu Thr Val 175 G u Asp Asp Sy Thr 255 Thr Pr o Al a G n Lys 335 Leu Asp SIe His Asp Leu IVet Leu 400 Page 134 Ser Tyr Arg u Ala 405 G u Al a y Ser Val 420 Thr His G y Lys Ala 435 G y lie Pro Ala Hi s 450 <210> 103 <211> 1287 <212> DNA <213> Corvnebacter i Gene Sequence 24-2-06.txt Pro G u G y n lie Leu His G u G y lie Asn 410 415 Al a Ser Phe I e Ala Ala y Thr Ser Tyr Ala 425 430 Mbt Ile Pro Leu Tyr Ile Phe Tyr Ser Met Phe 440 445 Ar g um al ut am cum <220> <221> <222> <223> CDS (1)..(1287) FRXA02897 <400> 103 gat ct c gat Asp Leu Asp 1 ccg t cc tac Pro Ser Tyr act gtg t cc Thr Val Ser ttc aac cgc Phe Asn Arg cac gtc tgg His Val Tr p cgt ggt ctc Arg G y Leu ttc gtg gtt Phe Val Val aac acc aag Asn Thr Lys 115 tgg tct gtg Trp Ser Val 130 gag aag gac G u Lys Asp 145 gat ggt gac Asp G y Asp ttc Phe 5 cac Hi s ggt Sy ct c Leu ttc Phe cag G n tgc Cys at c SIle aag Lys gat Asp cag G n 165 cgt cag gaa gtt Arg G n G u Val cgt gag cag ggt Arg Gu Gn G y cca Pr o ctt Leu gaa G u ctt Leu 70 cag G n aac Asn cag G n gtt Val ggt G y 150 acc Thr cac Hi s ggc Sy aac Asn ggc Sy gct Al a ct g Leu gaa G u gtt Val 135 gca Al a ttc Phe at g bet at g bet ggc Sy ggc Sy ct g Leu cgt Ar g 105 gag G u ggt G y gtt Val gct Al a gac Asp gcc Al a aag Lys at g et 75 aac Asn gac Asp ttc Phe gag G u at c SIle 155 gac Asp tgg Tr p t ac Tyr acc Thr gag G u gac Asp cct Pr o cgt Ar g 125 gat Asp aac Asn gca Al a ggc att G y le ttc cca Phe Pro gca cgt Al a Ar g gac cag Asp n gaa t ca Su Ser ctg acc Leu Thr cgc ggt Arg G y gca ggc Al a y ctt ctg Leu Leu acc tcc Thr Ser 160 gtt cgt Val Arg 175 Page 135 Gene Sequence 24-2-06.txt gag G u at g Mbt cgc Ar g cca Pr o 225 aac Asn gat Asp gag Gu gaa G u ggt Gy 305 cca Pr o cag G n cgc Ar g gca Al a aac Asn 385 t cc Ser gag G u ttc Phe gac Asp 195 gtt Val gt c Val gaa G u ct g Leu ct g Leu 275 gct Al a t ac Tyr ct g Leu gct Al a aag Lys 355 acc Thr cac Hi s cgt Ar g ggt Gy ttc gga cgt gac cca cgc Phe Gy Arg Asp Pro Arg 180 185 gaa gaa atc tgg aag ctt Gu Gu lie Trp Lys Leu 200 tac gca gcc tac aag cga Tyr Ala Ala Tyr Lys Arg 215 atc ctt gct cac acc att Ile Leu Ala His Thr Ile 230 ggc cgt aac gca acc cac G y Arg Asn Ala Thr Hi s 245 aag ttg ttc cgc gac aag Lys Leu Phe Arg Asp Lys 260 265 gag aag gat cct tac ctt G u Lys Asp Pro Tyr Leu 280 cct gaa atc aag tac atg Pro G u Ile Lys Tyr IVet 295 ctg cca gag cgt cgt gag Leu Pro G u Arg Arg G u 310 gat aag ctt cgc tct gtc Asp Lys Leu Arg Ser Val 325 acc acc atg gcg act gtt Thr Thr et Ala Thr Val 340 345 ggc ttg gct gat cgc ctt Sy Leu Al a Asp Arg Leu 360 ttc ggt ctt gac tct tgg Phe y Leu Asp Ser Trp 375 ggt cag aac tac gtg cct G y G n Asn Tyr Val Pro 390 gag gca cct gaa gga cag G u Ala Pro G u Gy n 405 acc gca Thr Al a aag ctc gtt gag aac Lys Leu Val G u Asn 190 cca Pr o gct Al a aag Lys cag G n 250 cag G n cct Pr o aag Lys aac Asn cgt Ar g 330 cgt Ar g gt c Val ttc Phe gtt Val at c SIle 410 cgt Ar g ctt Leu ggc Gy 235 at g Net ggc Gy cct Pr o gaa G u t ac Tyr 315 aag Lys acc Thr cca Pr o cca Pr o gac Asp 395 ct g Leu ggc Gy 205 acc Thr gga Gy aag Lys cca Pr o t ac Tyr 285 cgc Ar g cca Pr o t cc Ser aag Lys at t SIle 365 ttg Leu gac Asp gaa G u gat Asp gat Asp ggc Gy acg Thr 255 acc Thr cca Pr o gcg Al a cag G n aag Lys 335 ct g Leu gat Asp at c SIe at g bet at c SIe 415 624 672 720 768 816 864 912 960 1008 1056 1104 1152 1200 1248 1287 tcc gtg gca tcg ttc atc gct gcg ggt acc Ser Val Ala Ser Phe Ile Ala Ala Gy Thr 420 425 <210> 104 <211> 429 <212> PRT Page 136 Gene Sequence 24-2-06.txt <213> Corynebacter i um gl ut am cum Asp 1 Pr o Thr Phe Hi s Ar g Phe Asn Tr p G u 145 Asp G u let Ar g Pr o 225 Asn Asp G u G u Gy 305 Pr o G n 0> 104 Leu Asp G y Phe Arg n G u Val Tyr Ser Ar g Tr p Leu Val Lys 115 Val Asp Asp Phe Asp 195 Val Val G u Leu Leu 275 Al a Tyr Leu Al a 5 Hi s Gy Leu Phe G n Cys SIle Lys Asp G n 165 Gy G u Al a Leu Ar g 245 Leu Lys G u Pr o Lys 325 Thr Pr o Leu G u Leu 70 G n Asn G n Val Gy 150 Thr Ar g SIle Al a Al a 230 Asn Phe Asp SIle G u 310 Leu Nbet Hi s Gy Asn Gy Al a Leu G u Val 135 Al a Phe Asp Tr p Tyr 215 Hi s Al a Ar g Pr o Lys 295 Ar g Ar g Al a vet let Gy Gy Leu Ar g 105 G u Gy Val Al a Ar g 185 Leu Ar g SIle Hi s Lys 265 Leu bet G u Val Val 345 Ar g Asp Al a Lys let Asn Asp Phe G u SIle 155 Asp Al a Ar g Leu Gy 235 bet Gy Pr o G u Tyr 315 Lys G n Tr p Tyr Thr G u Asp Pr o Ar g 125 Asp Asn Al a Leu Sy 205 Thr Sy Lys Pr o Tyr 285 Ar g Pr o Ser Sy G u G n Ser Pr o Asn Val 110 Sy G u Asn Tyr Val 190 Hi s Lys Leu Leu SIle 270 Hi s Al a SIle Sy Sy Phe Al a Asp G u Leu Ar g Al a Leu Thr Val 175 G u Asp Asp Sy Thr 255 Thr Pr o Al a G n Lys 335 Arg Thr Phe Lys G u Leu Iet 350 Page 137 Arg Asp Lys y Leu Al a Asp 355 Ala Arg Thr Phe y Leu Asp 370 375 Asn Pro His G y G n Asn Tyr 385 390 Ser Tyr Arg G u Ala Pro G u 405 G u Ala y Ser Val Al a Ser 420 Gene Sequence 24-2-06.txt Arg Leu Val Pro Ile Ile Pro Asp Gu 360 365 Ser Trp Phe Pro Thr Leu Lys lie Tyr 380 Val Pro Val Asp His Asp Leu IVet Leu 395 400 G y G n lie Leu Hi s G u G y lie Asn 410 415 Phe I e Al a Al a y Thr 425 <210> 105 <211> 1133 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (88)..(1110) <223> RXN03083 <400> 105 attcagcagt aatcatttag acttggaacc gcttaccagt ggtttcaaca atgcattcac ccagctcaca cgtgtggagg tgcctta atg gca aag agg atc gta att atc ggc NIet Ala Lys Arg Ile Val Ile Ile Gy 1 ggt Gy gca Al a acc Thr aaa Lys gga Gy ctt Leu gat Asp aag Lys gaa G u cct Pr o gtt Val gac Asp gac Asp gca Al a aaa Lys cgc Ar g acc Thr gtt Val ggc Gy gtt Val gt a Val cga Ar g ct a Leu cag G n at t SIle 110 cac Hi s tgc Cys gaa G u gaa G u t ca Ser gct Al a at c SIle gaa G u ggt Gy at t SIle ct g Leu gcc Al a gat Asp aag Lys gat Asp 65 gat Asp gat Asp gt g Val aaa Lys gt g Val gca ctc gca ggc gct aaa tac ggt Ala Leu Al a G y Al a Lys Tyr G y 20 gtc gga gtt ggc gga tcc gca gtc Val y Val Gy Gy Ser Al a Val 35 tcc ttc atc gct ggt acc ggt atc Ser Phe lie Ala y Thr Gy Ile gac atg gga ctt aac cgt ggg ctt Asp IVet Gy Leu Asn Arg y Leu gca ctg aac atc cgt gtg aag gac Ala Leu Asn lie Arg Val Lys Asp atc ttg ggc cag ctg cag cgc tca Ile Leu Gy G n Leu G n Arg Ser 100 105 ggc cgc ttt gat gat tac aac acc Sy Arg Phe Asp Asp Tyr Asn Thr 115 120 gtc acc cac agc gat ggc tcc gaa Val Thr His Ser Asp G y Ser G u 130 135 ctg gtt gca act ggt gca acc ccc Leu Val Ala Thr y Ala Thr Pro Page 138 114 162 210 258 306 354 402 450 498 546 Gene Sequence 24-2-06.txt cgc Ar g cgt Ar g 170 ggt Gy ggc Gy gat Asp gga Gy gaa G u 250 ggt Gy ctt Leu aag Lys gt g Val gcc Al a 330 ctt Leu gt c Val ggt Gy aaa Lys gca Al a 220 t cc Ser ggt Gy cac Hi s ct g Leu gac Asp 300 gt a Val tcg Ser ggt Gy gac Asp acc Thr 190 acc Thr gcc Al a gaa G u gt g Val ttg Leu 270 aac Asn tct Ser acc Thr gca Al a at t SIle 175 ggt Gy at g bet gca Al a aag Lys tgt Cys 255 at g bet at c SIle ccc Pr o t at Ty r gag G u 160 gaa G u gcg Al a gt g Val gac Asp cat Hi s 240 gtt Val act Thr ggt Gy gca Al a t cc Ser 320 145 cca Pr o gaa G u gaa G u gca Al a gt g Val 225 gcc Al a cgc Ar g gtt Val gtt Val cca Pr o 305 cac Hi s gac Asp ct c Leu ttt Phe t cc Ser 210 ct g Leu cgc Ar g act Thr ggt Gy gag G u 290 aca Thr tgg Tr p gag G u acc Thr 180 tct Ser gac Asp acc Thr gag G u gac Asp 260 at t SIle gca Al a ccg Pr o ccg Pr o cgc Ar g 165 cac Hi s gcg Al a cgc Ar g gtt Val tct Ser 245 gga Gy cca Pr o cca Pr o gt g Val ttg Leu 325 150 at c SIle ctt Leu ttt Phe at t SIle ct g Leu 230 gt c Val cga Ar g aac Asn t cc Ser tgt Cys 310 cag G n ttg Leu at c SIle gct Al a ttg Leu 215 gct Al a acc Thr gaa Gu acg Thr ggc Gy 295 acg Thr cga Ar g acc Thr gt g Val gaa G u 200 cct Pr o gag G u cgc Ar g at c SIe gca Al a 280 cat Hi s cag G n tgc Cys 594 642 690 738 786 834 882 930 978 1026 1074 1120 1133 tgt atc Cys I I e 335 acg cac tcg gtg Thr His Ser Val aag gcg Lys Al a 340 tgagccccat ccgtttgaag act <210> 106 <211> 341 <212> PRT <213> Corynebacter i um gl <400> 106 IVet Ala Lys Arg Ile Val 1 5 Al a Al a Leu Al a y Al a Asp Val Gy Val Gy Gy ut ari cum Ile Ile Gy Gy y Pro Ala y Tyr u 10 Lys Tyr Gy Ala u Val Thr Val Ile u 25 Ser Ala Val Thr Wet Asp Cys Val Pro Ser 40 Page 139 Lys Ser Phe IlieAla Gy Thr 55 ene Sequence 24- 2- 06. txt Gy Ilie Lys Thr Asp Leu Arg Arg Ala As p As p As p ValI Ly s ValI 145 Pr o O31u O31u A a ValI 225 A a Ar g ValI ValI Pr o 305 Hi s Hi s vb t Le u Le u Ar g 115 Thr ValI G31y Pr o ValI 1 95 Ar g O31u ValI A a Ser 275 Le u Ser Ar g ValI G3 y Leu Asn I I e G31y (1n 1 00 Phe As p Hi s Ser A a Thr O31u Ar g 1 65 Thr Hi s 1 80 Ser A a Asp Arg Thr Val G3 u Ser 245 As p (1y 260 li1e Pr o Ala Pr o Pr o Val Pro Leu 325 Lys A a 340 Ar g ValI O31n Tyr G31y 135 A a Le u I Ie A a Le u 215 A a Thr O31u Thr G31y 295 Thr Le u As p Ser 1 05 Thr O31u Pr o Tr p ValI 1 85 Le u Hi s Ar g Thr Tyr 265 As p I Ie O31n Lys5 75 A a ValI O31n Thr I Ie 1 55 O31n Ser ValI As p ValI 235 As p Ser O31y ValI Thr 315 A a Lys5 Ar g Thr ValI 140 Le u ValI O31y Lys5 A a 220 Ser O31y Hi s Le u As p 300 ValI Hi s A a vb t Thr 125 O31u Lys5 Tyr ValI ValI 205 As p Le u O31y A a O31u 285 Ar g Le u Le u O31n I Ie 110 Hi s cys O31y As p Thr 1 Thr A a O31u ValI Le u 270 As n Ser Thr O31u Ser As n Tyr As p A a I Ie 1 O31y A a Lys5 cys 255 vb t I Ie Pr o Tyr lIe O31u O31y Le u O31u 1 O31u A a ValI As p Hi s 240 ValI Thr O31y A a Ser 320 Arg Cys Arg Ala Val Ser Pro Cys lieThr 330 335 <210O> 107 <21 1> 1518 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1495) <223> FRXA02853 Page 140 17 ene Sequence 24- 2- 06. txt aat tcagcag t aat cat t ta gact tggaac cgct taccag t ggt t tcaac aat gcat tca cccagct cac acgt gt ggag gt gcct ta at g gca aag agg at c gt a at t at c ggc 115 I'bt Ala Lys Arg lieVal Ilie Ilie y ggt G31y gca Al a ac c Thr aaa Ly s gga G31y ct t Le u g at As p aag Ly s g aa (31u cgc Ar g cgt Ar g 1 70 ggt G31y ggc G31y g at As p gga G31y cct Pr o gt t ValI g ac As p g ac As p gca Al a aaa Ly s cgc Ar g ac c Thr gt t ValI 140 ct t Le u gt c ValI ggt G31y aaa Ly s gca Al a 220 t cc Ser ggc G31y gt t ValI gt a ValI cga Ar g ct a Le u c ag O31n at t I Ie 110 c ac Hi s t gc cys ggt G31y g ac As p ac c Thr 1 90 ac c Thr gcc Al a g aa (31u t at Tyr 15 at t I Ie cct Pr o cgt Ar g gaa (31u t cc Ser 95 aac As n t ac Tyr g at As p gca Al a at t I Ie 1 75 ggt G31y at g vb t gca Al a aag Ly s g aa (31u g aa (31u t ca Ser gct Al a at c I Ie g aa (31u ggt G31y at t I Ie ct g Le u gag (31u 1 60 g aa (31u gcg Al a gt g ValI g ac As p cat Hi s 240 gcc Al a g at As p aag Ly s g at As p 65 g at As p g at As p gt g ValI aaa Ly s gt g ValI 145 cca Pr o g aa (31u g aa (31u gca Al a gt g ValI 225 gcc Al a gca Al a gt c ValI t cc Ser g ac As p gca Al a at c G31y gt c ValI 130 ct g Le u g ac As p ct c Le u tt t Ph e t cc Ser 210 ct g Le u cgc Ar g ct c gca Leu Al a 20 gga gtt 03 y Val 35 t tc at c Phe I I e at g gga [Vb t (1y ct g aac Leu Asn ttg ggc Leu 03 y 1 00 cgc ttt Ar g Phe 115 acc cac Thr Hi s gt t gca Val Al a ggc gag (31y (1u ccc acc Pr o Thr 1 80 gt c t ct Val Ser 1 95 cgt gac Ar g As p gaa acc 03 u Thr gt g gag Val (31u ggc G31y ggc G31y gct Al a ct t Le u at c I Ie c ag O31n g at As p ag c Ser act Thr cgc Ar g 1 c ac Hi s gcg Al a cgc Ar g gt t ValI t ct Ser 245 gct Al a gga G31y ggt G31y aac As n cgt Ar g ct g Le u g at As p g at As p ggt G31y 1 at c I Ie ct t Le u tt t Ph e at t I Ie ct g Le u 230 gt c ValI aaa Ly s t cc Ser ac c Thr cgt Ar g gt g ValI c ag O31n t ac Tyr G31y 135 gca Al a tt g Le u at c I Ie gct Al a tt g Le u 215 gct Al a ac c Thr t ac Tyr gca Al a ggt G31y ggg G31y aag Ly s cgc Ar g aac As n 120 t cc Ser ac c Thr ac c Thr gt g ValI g aa (31u 200 cct Pr a gag (31u cgc Ar g ggt G31y gt c ValI at c I Ie ct t Le u g ac As p t ca Ser 1 ac c Thr g aa (31u ccc Pr a t gg Tr p gt t ValI 1 ct c Le u c ac Hi s cgc Ar g ac c Th r gaa gac ggt ggc gt g t gt gt t cgc act gct gac gga cga gaa at c t ac Page 141 G u Asp G y G y Val 250 Cys Val 255 Gene Sequence 24-2-06.txt Arg Thr Ala Asp Gy Arg Gu lie ggt t ct cac G y Ser Hi s ctt ggc ctg Leu G y Leu aag gtt gac Lys Val Asp 300 ggt gac tgt G y Asp Cys 315 ggc cgt at c Sy Arg lle 330 cgt ttg aag Arg Leu Lys gca gt a ggt Ala Val y cgc gtg at t Arg Val lle 380 t cc t g cgc Ser Leu Arg 395 ct g at c at c Leu lie lle 410 ct a ccg at c Leu Pro Ile gct gat acc Al a Asp Thr gca gca cgt Al a Al a Ar g 460 ttg Leu 270 aac Asn gt c Val gac Asp at g bet gtt Val 350 acc Thr ctt Leu ggt Sy ggt Sy gt g Val 430 gcg Al a at g bet at c SIle t cc Ser ct a Leu t at Ty r 335 gcc Al a cat Hi s cct Pr o ttt Phe gt c Val 415 gca Al a gt g Val act Thr ggt G y cgc Ar g ttc Phe 320 cac Hi s acc Thr gca Al a ttg Leu gt g Val 400 gt g Val gt g Val t ac Tyr gtt Val gtt Val acc Thr 305 cca Pr o gca Al a gca Al a caa G n gct Al a 385 aag Lys gt g Val acc Thr cca Pr o cat Hi s 465 ggt G y gag G u 290 aac Asn ct g Leu ct c Leu gt g Val gtt Val 370 act Thr ct g Leu gca Al a aac Asn t ca Ser 450 at t SIle gca Al a ccc Pr o t cc Ser gaa G u 340 acc Thr t cc Ser cca Pr o tgc Cys acc Thr 420 ct g Leu t ca Ser cca Pr o cca Pr o ggt G y gtt Val 325 ggc G y cgc Ar g ggc G y cgc Ar g cgc Ar g 405 gcg Al a aca Thr ggt G y aac Asn t cc Ser gt g Val 310 gca Al a gt g Val cca Pr o gaa G u gcc Al a 390 cgt Ar g tct Ser gtt Val tcg Ser acg Thr ggc G y 295 t ac Tyr gcg Al a agc Ser gag G u gt g Val 375 aag Lys aac Asn gag G u gct Al a at t SIle 455 gca Al a 280 cat Hi s gca Al a at g bet ccc Pr o at c SIle 360 t ct Ser at g Net tct Ser ct g Leu gat Asp 440 act Thr gat Asp at c SIe gca Al a cag G n at c SIe 345 gca Al a gct Al a cgt Ar g ggc G y at c SIe 425 ct g Leu gaa G u 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1505 1518 ctg gtt caa Leu Val G n gat gat cta ggc Asp Asp Leu G y t aat t t t t ct gagtcttaga ttt <210> 108 <211> 469 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 108 IVet Ala Lys Arg Ile Val Ile Ile Gy Gy y Pro Ala y Tyr u 1 5 10 Ala Ala Leu Ala y Ala Lys Tyr Gy Ala Gu Val Thr Val Ile u Page 142 Gene Sequence 24-2-06.txt Val Pro Ser Asp Lys Asp Asp Asp Val Lys Val 145 Pr o G u G u Al a Val 225 Al a Ar g Val Val Thr 305 Pr o Al a Al a G n Gy Phe bet Leu Leu Ar g 115 Thr Val Gy Pr o Val 195 Ar g G u Val Al a Ser 275 Leu SIle Al a Gy Phe 355 Asp Gy Al a Leu SIle G n Asp Ser Thr Ar g 165 Hi s Al a Ar g Val Ser 245 Gy Pr o Pr o Gy Val 325 Gy Ar g Gy Ser Thr Ar g Val G n Tyr Gy 135 Al a Leu SIle Al a Leu 215 Al a Thr G u Thr Gy 295 Tyr Al a Ser G u Val 375 Al a 40 Gy Gy Lys Ar g Asn 120 Ser Thr Thr Val G u 200 Pr o G u Ar g SIle Al a 280 Hi s Al a bet Pr o SIle 360 Ser Val SIle Leu Asp Ser 105 Thr G u Pr o Tr p Val 185 Leu Hi s Ar g Thr Tyr 265 Asp SIle Al a G n SIle 345 Al a Al a Thr Net Asp Cys Lys Thr Asp Leu G y Lys Al a Hi s Leu Ala Lys Ala 90 Asp Val Arg IVet Lys G n Thr Thr 125 G u Thr Val G u 140 Arg lie Leu Lys 155 Arg G n Val Tyr 170 G y Ser G y Val G y Val Lys Val 205 Asp Asp Al a Asp 220 G y Val Ser Leu 235 Su Asp G y y 250 G y Ser His Al a Leu G y Leu G u 285 Lys Val Asp Arg 300 G y Asp Cys Thr 315 Sy Arg lie Al a 330 Arg Leu Lys Thr Ala Val Gy Ile 365 Ar g Leu G n SI e 110 Hi s Cys Gy Asp Thr 190 Thr Al a G u Val Leu 270 Asn Val Asp bet Val 350 Thr Ar g G u Ser Asn Tyr Asp Al a SIe 175 Gy et Al a Lys Cys 255 bet SIe Ser Leu Tyr 335 Al a Hi s Arg Val lie Val Leu Pro Leu 380 Page 143 Gene Sequence 24 Ala Thr Asn Pro Arg Ala Lys IVet Arg Ser Leu 385 390 395 Lys Leu Phe Cys Arg Arg Asn Ser y Leu lie 405 410 Val Ala Pro Thr Ala Ser G u Leu Ile Leu Pro 420 425 Thr Asn Arg Leu Thr Val Ala Asp Leu Ala Asp 435 440 Pro Ser Leu Ser y Ser Ile Thr G u Ala Al a 450 455 Hi s Asp Asp Leu G y 465 <210> 109 <211> 2895 <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(2872) <223> RXA02259 <400> 109 atgagcccat gaaagccatc gaaatcaatc gcccagctaa ttttttatct catgcacgcc aacaccctca atgtgaaaga -2-06. txt Arg His Gy Phe Val 400 I e Gy y Val Val 415 Ile Ala Val Ala Val 430 Thr Phe Ala Val Tyr 445 Arg G n Leu Val G n 460 acacctgttt tgctgggtga gtg ttt aaa gta gtt 11! Val Phe Lys Val Val at g bet ggt Gy gaa G u at g Net aca Thr gcg Al a ggc Gy aat Asn gat Asp gt a Val gcg Al a agc Ser at t SIle gac Asp acc Thr ggc Gy 120 tta Leu gcg Al a ct g Leu gtt Val cgc Ar g t ac Tyr ccg Pr o gtt Val cgc Ar g gaa Gu act Thr cag G n gca Al a 75 gat Asp gac Asp ggc Gy gat Asp caa Gn tct Ser gtt Val ttt Phe gaa G u agc Ser gca Al a gac Asp gaa Gu ttt Phe 45 ttc Phe t cc Ser gag G u act Thr gaa 15u 125 at c SIle ggc Gy gat Asp gac Asp cac Hi s ctt Leu ctt Leu 110 gct Al a ttc Phe gag Gu gcc Al a at t SIle gct Al a 80 gaa G u gcc Al a gcc Al a ggt Sy t at Tyr ggc Sy cca Pr o ct g Leu gct Al a tgg Tr p gt g Val 130 caa G n gaa Gu aac Asn gcc Al a gct Al a ct c Leu ct g Leu 115 ct g Leu at c I e ct g Leu gcc Al a aag Lys aac Asn gat Asp 100 aaa Lys cgc Ar g ct c Leu gtc Val gaa G u gca Al a ctg Leu gca Al a ct c Leu aat Asn Page 144 Gene Sequence 24-2-06.txt gct gag gtg gcg ccg gtt ctg act Ala u Val Ala Pro Val Leu Thr cgc Ar g 150 cgc Ar g ttg Leu cag G n gaa G u cgt Ar g 230 ggt Gy gac Asp act Thr cat Hi s acc Thr 310 agc Ser cgt Ar g ggc Gy tta Leu gtt Val 390 gtt Val gct Al a gag G u gcg Al a 200 ggg Gy aac Asn cct Pr o gac Asp cgc Ar g 280 ct c Leu cag G n gt g Val ct c Leu tgg Tr p 360 gat Asp at t SIle gat Asp cag G n 170 gag G u at t l Ie cgc Ar g gat Asp aag Lys 250 aac Asn gcg Al a cat Hi s ctt Leu gag G u 330 acg Thr aag Lys ttg Leu gat Asp gcg Al a 155 tct Ser aag Lys cgt Ar g tac Ty r gt g Val 235 ccc Pr o cct Pr o gaa G u gag G u gcg Al a 315 cct Pr o acg Thr gt c Val acc Thr gat Asp 395 140 caa G n gcg Al a aac Asn gt g Val t ac Tyr 220 gct Al a gt g Val t at Tyr acc Thr ct c Leu 300 ct g Leu t at Tyr gcc Al a ttt Phe at t SIle 380 cgt Ar g aag Lys gag G u at c lile gcc Al a 205 aag Lys gtt Val gt c Val gt c Val gt g Val 285 agc Ser gca Al a cga Ar g gag G u act Thr 365 gat Asp ttg Leu gcg Al a tgg Tr p cct Pr o cgc Ar g 190 cgc Ar g ct g Leu gag Gu aag Lys acc Thr 270 ct c Leu ct g Leu gat Asp cgc Ar g ct g Leu 350 cca Pr o cat Hi s tct Ser cac cca act Hi s Pro Thr at c SIle acc Thr 175 cgt Ar g cca Pr o agc Ser ctt Leu cca Pr o 255 gcg Al a aag Lys tcg Ser gca Al a gcc Al a 335 at c SIle t ac Tyr tct Ser gt g Val acc Thr 160 gct Al a cgc Ar g cgt Ar g ctt Leu cgt Ar g 240 ggt Gy gaa G u t ac Tyr gac Asp ggg Sy 320 gt c Val ggc Gy gca Al a ct g Leu ct g Leu 400 cac Hi s acg Thr acc Thr gag G u 210 gaa G u cgt Ar g tgg Tr p gtt Val gca Al a 290 at g Met aac Asn ggc Gy gac Asp ccg Pr o 370 gaa G u tct Ser gag act cgc cgc G u Thr Arg Arg at g Mbt caa G n at t SIle 195 gac Asp gag G u ttc Phe at t SIle gag G u 275 cgc Ar g aat Asn gac Asp gtt Val gcc Al a 355 gaa G u t cc Ser gcc Al a cgt Ar g agc Ser 180 ttg Leu gag G u at t lle ggc Gy ggt Gy 260 t at Tyr cag G n aag Lys gt g Val cgc Ar g 340 gtt Val gaa G u aag Lys at c SIe 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 Page 145 Gene Sequence 24-2-06.txt agc ttt gga Ser Phe G y ttc aac ctt tac Phe Asn Leu Tyr agc Ser gca Al a aag Lys t ac Tyr 470 gag G u t cc Ser aag Lys gt c Val gcc Al a 550 ct c Leu t cc Ser gcg Al a cgc Ar g t cc Ser 630 gt g Val gag G u t ac Tyr 440 ct g Leu gag G u gtt Val gca Al a ttc Phe 520 gt c Val at c l Ie cag G n aag Lys ct g Leu 600 ttc Phe gac Asp at c SIle 410 gt c Val gag G u agc Ser acc Thr aaa Lys 490 tcg Ser ct c Leu cca Pr o gac Asp gac Asp 570 ggc Gy ct c Leu ggc Gy at t SIle gag G0u 650 ct c Leu ct g Leu cct Pr o gac Asp 475 tt c Phe gt c Val at c SIle ct g Leu gaa G u 555 aac Asn gga Gy gt c Val cgt Ar g ctt Leu 635 cag G n acc Thr tct Ser cgt Ar g 460 cgc Ar g ggg G y acc Thr gca Al a ttc Phe 540 ct g Leu gt c Val t at Tyr gaa G u ggt G y 620 gcc Al a ggc G y gca Al a gag G u gaa G u 445 ccg Pr o gag G u cca Pr o gat Asp gcc Al a 525 gaa G u tgg Tr p cag G n ttc Phe ct a Leu 605 ggc Gy cag G n gag G u ctg gat Leu Asp 415 ctg cgc caa aac tcc gaa Leu Arg G n Asn Ser G u 420 ctt Leu 430 gca Al a ct g Leu ct c Leu cgg Ar g gt g Val 510 aac Asn acc Thr aaa Lys gaa G u t cc Ser 590 tgc Cys acc Thr ccc Pr o at c SIle ttc Phe gag G u at c SIle ggc G y at g let 495 ct c Leu ggc Sy at c SIle at t SIle gt c Val 575 gca Al a cga Ar g gt c Val agg Ar g at c SIle 655 gcc Al a gaa G u aag Lys ccg Pr o at c SIle 480 gt g Val gag Gu gac Asp gaa G u gat Asp 560 at g Met aac Asn t ca Ser ggc G y ggg G y 640 t cc Ser ct g Leu cgc Ar g ctt Leu cac Hi s 465 ttc Phe cct Pr o ccg Pr o aac Asn gat Asp 545 ct c Leu ct c Leu tgg Tr p gcc Al a cgc Ar g 625 gct Al a gct Al a gcc Al a gag G u 450 ggt G y cgc Ar g cac Hi s at g bet cca Pr o 530 ct c Leu t ac Tyr ggt Sy gcg Al a ggg G y 610 ggt G y gt c Val aag Lys caa G n 435 gt g Val t ca Ser acc Thr tgc Cys gt g Val 515 cgc Ar g cag G n cgc Ar g t ac Tyr ctt Leu 595 gt c Val ggc G y caa G n t ac Tyr gt c Val ct g Leu gat Asp gcg Al a at c SIe 500 ttg Leu ggc Sy gcc Al a aac Asn t cc Ser 580 t ac Tyr aag Lys gga G y ggt G y ggc G0y 660 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 ccc gaa acc Pro G u Thr cgc cga aac ctc gaa Arg Arg Asn Leu G u 670 gtc tca gcc acg ctt Val Ser Ala Thr Leu 675 Page 146 Gene Sequence 24- 2- 06. txt gag gca tcg ctt ctc gac gtc tcc gaa ctc acc gat cac caa cgc gcg G u Ala Ser Leu Leu Asp Val Ser G u Leu Thr Asp His n Arg Ala 680 t ac gac at c Tyr Asp II e 695 tcc ttg gtg Ser Leu Val 710 acg ccg ctg Thr Pro Leu t ca cgc aag Ser Arg Lys gtg ctc agc Val Leu Ser 760 gt c gga acc Val G y Thr 775 caa cgc at t Sn Arg lle 790 acc t ca gt g Thr Ser Val cgt ttg gca Arg Leu Ala gag cga gt c Su Arg Val 840 atg ttc tgc Ibet Phe Cys 855 ctt ctc gca Leu Leu Al a 870 aac gt g at c Asn Val lle agc gag caa Ser 0 u 0 n act gcg ct g Thr Ala Leu 920 <210> 110 <211> 924 <212> PRT agt Ser gag G u gag G u 730 acc Thr t ca Ser tta Leu gag G u gat Asp 810 ct c Leu t cc Ser at c SIle tct Ser gt a Val 890 t cc Ser at c SIle 700 caa G n gga Gy tcg Ser tct Ser cag G n 780 caa G n at g et gca Al a at c SIle ggc Sy 860 cag G n at g bet aac Asn 685 tct Ser ggc G y t cc Ser gt g Val cgt Ar g 765 tgg Tr p aca Thr gct Al a gac Asp cgc Ar g 845 tct Ser cgc Ar g at g bet at t SIle gag ctc agc G u Leu Ser ttc atc gat Phe Ile Asp 720 ct c aac at c Leu Asn Ile 735 gaa gat ttg G u Asp Leu 750 gtc atg ct g Val et Leu att ggc gaa lie y G u ct c aat gag Leu Asn G u 800 cag gtg at g G n Val vbet 815 ct g at c cca Leu Ile Pro 830 gag gag tac G u G u Tyr gat gat ctg Asp Asp Leu cga t ac ccc Arg Tyr Pro 880 cga cgc tac Arg Arg Tyr 895 cag ctg acc G n Leu Thr 910 ttg Leu 705 t ac Tyr gga G y cga Ar g cca Pr o ggg Gy 785 t cc Ser t cc Ser gat Asp ttc Phe ctt Leu 865 t ac Tyr cga Ar g at g bet 690 aag aag Lys Lys ttc acc Phe Thr tcc agg Ser Arg gcc at c Ala I e 755 ggc tgg Sy Trp 770 gag cag G u G n tgg cca Trp Pro aag gca Lys Al a acg gaa Thr G u 835 ctg acc Leu Thr 850 gat gac Asp Asp ctg ctt Leu Leu aaa ggc Lys G y aac ggt Asn y 915 t ac Tyr cag G n cct Pr o 740 cca Pr o ttt Phe gcc Al a ttt Phe gag G u 820 gt a Val aag Lys aac Asn cca Pr o gac Asp 900 ctt Leu gcc Al a t cc Ser 725 t cc Ser tgg Tr p ggt G y acc Thr ttc Phe 805 ct g Leu gcc Al a aag Lys cca Pr o ct c Leu 885 caa G n t cc Ser 2179 2227 2275 2323 2371 2419 2467 2515 2563 2611 2659 2707 2755 2803 2851 2895 cgc aac tcc ggc Arg Asn Ser y tagtccagcc ggctgggtag tac Page 147 Gene Sequence 24-2-06.txt <213> Corynebacter i um gl ut ai cum <400> 110 Val Phe Lys 1 Leu Val Lys Thr Leu G n Thr Asp Thr 145 Thr Ar g SIle SIle Leu 225 G u Ser Thr Tyr Ar g 305 Hi s Hi s Gn G u Asn Al a Al a Leu Leu 115 Leu Thr vet G n SIle 195 Asp G u Phe SIle G u 275 Ar g Asn Asp Val Val IVet Thr Asp Phe Leu 5 10 Leu Gy Gu Val I e Ala Val G u G n Ala Arg Leu 40 G u IVet Asp Ser Leu Val Ala Thr Pro Ile Ala Arg 70 Leu Al a G u Asp Leu Tyr 90 Ala G y Asp Thr Pro Pro 105 Leu Asn G u G y Asn Val 120 Asn Al a G u Val Ala Pro 135 Arg Arg Thr Val Phe Asp 150 G u Arg His Ala Leu G n 165 170 Lys Leu Asp G u lie G u 185 Trp n Thr Ala Leu Ile 200 I e u Val y Leu Arg 215 Pro Arg lie Asn Arg Asp 230 G u G y Val Pro Leu Lys 245 250 G y Asp Hi s Asp G y Asn 265 Ser Thr His Arg Ala Ala 280 Leu His Ser Leu G u His 295 Val Thr Pro G n Leu Leu 310 Pro Ser Arg Val Asp G u 325 330 G y Arg lie Leu Ala Thr 345 Ar g Gu Thr G n Al a 75 Asp Asp Sy Val Al a 155 Ser Lys Ar g Tyr Val 235 Pr o Pr o G u G u Al a 315 Pr o Asp G n Ser Val Phe G u Ser Al a Leu 140 G n Al a Asn Val Tyr 220 Al a Val Tyr Thr Leu 300 Leu Tyr Asp Gu Phe Phe Ser G u Thr G u 125 Thr Lys Su I e Al a 205 Lys Val Val Val Val 285 Ser Al a Ar g SIle Gy Asp Asp Hi s Leu Leu 110 Al a Al a Tr p Pr o Ar g 190 Ar g Leu G u Lys Thr 270 Leu Leu Asp Ar g Ar g Gn SIle Sy Phe Ar g Asp Val Hi s SIle Thr 175 Ar g Pr o Ser Leu Pr o 255 Al a Lys Ser Al a Al a 335 Phe Gu Al a SIe Al a G u Al a Al a Pr o Thr 160 Al a Ar g Ar g Leu Ar g 240 Sy G u Tyr Asp Sy 320 Val Thr Ala u Leu Ile y 350 Page 148 ene Sequence 24- 2- 06. txt Ou Asp AlaVal OuOy Val Phe Lys Val Phe Thr Pro Tyr Al a 365 Ser Ar g 385 li1e Ar g Ar g Le u Hi s 465 Ph e Pr o Pr o As n As p 545 Le u Le u Tr p Al a Ar g 625 Al a Al a ValI As p 355 O31u Ser Al a As n O31n 435 ValI Ser Thr cys ValI 515 Ar g O31n Ar g Tyr Le u 595 ValI G31y O31n Tyr Al a 675 O31n Ph e As p O31u 405 O31u Thr Le u O31u Ser 485 li1e Le u Thr O31y Tyr 565 As p As p Le u Pr o Ser 645 As n Le u Al a As n 375 Le u Ph e Tyr As n O31u 455 Ser Al a vb t O31u As p 535 O31y Le u As n O31u Le u 615 Tyr Ar g O31u Al a As p 695 Al a Al a Ph e As p 425 Ar g Ar g ValI Ly s Ser 505 O31y I Ie Le u Ar g As p 585 O31n Hi s Al a Thr Al a 665 Le u vb t Thr As p 395 Le u Le u Le u Pr a As p 475 Ph e ValI I Ie Le u O31u 555 As n O31y ValI Ar g Le u 635 O31n Ar g As p O31u I Ie 380 Ar g Tyr Thr Ser Ar g 460 Ar g G y Thr Al a Ph e 540 Le u ValI Tyr Ou Oy 620 Al a O3y As n ValI I Ie 700 As p Le u Al a O31u O31u 445 Pr a O31u Pr a As p Al a 525 O31u Tr p O31n Ph e Le u 605 O31y O31n O31u Le u Ser 685 Ser Ser ValI As p 415 Ph e Ou le Oy vb t 495 Le u Oy le le ValI 575 Al a Ar g ValI Ar g I Ie 655 Al a Le u Le u Leu Lys Lys Tyr Al a Ser Leu Val Hi s O31u Asp O31n O31y Phe I Ie Asp Page 149 Gene Sequence 24-2-06.txt 705 Tyr Gy Ar g Pr o Gy 785 Ser Ser Asp Phe Leu 865 Tyr Ar g bet 715 lie y Ser Leu Thr Ar g SIle 755 Tr p G n Pr o Al a G u 835 Thr Asp Leu Gy Gy 915 Ser 725 Ser Tr p Gy Thr Phe 805 Leu Al a Lys Pr o Leu 885 G n Ser Pr o Ar g Leu Gy 775 Ar g Ser Leu Ar g Phe 855 Leu Val G u Al a Leu G n G u 730 Lys G n Thr 745 Ser Trp Ser 760 Thr Al a Leu Ile Ala G u Val Leu Asp 810 Ala Lys Leu 825 Val Tyr Ser 840 Cys Val I le Al a Arg Ser Ile G n Val 890 G n Val Ser 905 Leu Arg Asn 920 Ser G n G u Leu 795 Asn Tyr Val Thr Val 875 G u Ar g Ser Val Ar g 765 Tr p Thr Al a Asp Ar g 845 Ser Ar g Iet I le 720 Asn I e 735 Asp Leu IVet Leu G y G u Asn u 800 Val bet 815 lie Pro G u Tyr Asp Leu Tyr Pro 880 Arg Tyr 895 Leu Thr <210> 111 <211> 939 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(916) <223> RXN02326 <400> 111 ccaggcggac agttgtccaa cctgcgtgca caggccaccg cactgggcct tgcggatcgt ttcgaactca tcgaagacaa ctacgcaagc cgttaatgag atg ctg gga cgc cca IVet Leu Gy Arg Pro 1 acc aag gtc acc cca tcc tcc aag gtt gtt ggc gac ctc gca ctc cac Thr Lys Val Thr Pro Ser Ser Lys Val Val G y Asp Leu Ala Leu His 15 ctc gtt ggt gcg ggt gtg gat cca gca gac ttt gct gcc gat cca caa Leu Val y Ala y Val Asp Pro Ala Asp Phe Ala Ala Asp Pro G n 30 aag tac gac atc cca gac tct gtc atc gcg ttc ctg cgc ggc gag ctt Page 150 Lys Tyr Asp Ile Pro Asp Ser Gene Sequence 24- 2- 06. txt Val I e Ala Phe Leu Arg Gy u Leu ggt Sy gaa Gu gag Gu ct c Leu cgt Ar g t ac Tyr cgc Ar g 150 aag Lys at g et aag Lys gtt Val gca Al a 230 tct Ser cct Pr o cgc Ar g gcg Al a cgc Ar g cgc Ar g 120 ct g Leu cca Pr o at g Met gt g Val gat Asp 200 acc Thr gca Al a gac Asp ggt Sy gaa Gu ct c Leu ct g Leu ggc Gy gaa Gu ctt Leu aat Asn 170 gac Asp t cc Ser act Thr at c l Ie aaa Lys 250 tgg Tr p aag Lys gct Al a ccg Pr o acc Thr cgc Ar g 140 cgc Ar g gt g Val t cc Ser aag Lys gct Al a 220 gct Al a gat Asp cca Pr o gca Al a gat Asp aag Lys tct Ser 125 gag Gu ct g Leu gcc Al a gtt Val ggc G y 205 gaa Gu at g Net cgc Ar g gag G u cct Pr o gat Asp cca Pr o 110 gcg Al a act Thr gat Asp aac Asn gag G u 190 cat Hi s ggt G y aag Lys gtt Val cca Pr o ct g Leu t cc Ser 95 acc Thr ct g Leu ttg Leu gcg Al a gt c Val 175 tct Ser gtt Val gat Asp at g Net gt g Val 255 ct g Leu acg Thr 80 aag Lys gaa Gu gat Asp at c SIle at c l Ie 160 aac Asn gt c Val gct Al a gag G u gaa Gu 240 gtt Val cgc Ar g gaa Gu gaa Gu gag Gu gat Asp cgc Ar g 145 tct Ser ggc G y acc Thr gca Al a gt c Val 225 gca Al a cct Pr o acc Thr gtt Val cgt Ar g ttc Phe cgt Ar g 130 ct g Leu gag G u cag G n gca Al a cca Pr o 210 aag Lys aca Thr gct Al a cgc Ar g cct Pr o cgc Ar g ct c Leu 115 gaa Gu cca Pr o cca Pr o at c SIle acc Thr 195 ttc Phe gct Al a at c SIle gca Al a gca ctg Al a Leu gag gaa Gu Gu aat agc Asn Ser 100 gag cac G u Hi s ttc ttc Phe Phe gat gtg Asp Val gac gat Asp Asp 165 cgc cca Arg Pro 180 gca gaa Al a u gct ggt Al a y gga gat G y Asp act gct Thr Al a 245 acg aag Thr Lys 260 gtg gaa ggt ggc gac ttg atc gtc gtc gtt tcc Val G u Gy y Asp Leu Ile Val Val Val Ser 265 270 ccc <210> 112 <211> 272 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 112 taaacctttc tgtaaaaagc 936 Page 151 l'bt Leu 31y Ar g Pr o Thr Lys 1 5 ene Sequence 24- 2- 06. t xt Val Thr Pr o Ser Ser Lys Val Val G31y 10 As p A a Le u Ar g (31u (31u (31u As p Ar g 145 Ser G31y Thr A a ValI 225 A a Pr o A a As p G31y Ar g Pr o Ar g Le u 115 (31u Pr o Pr a I Ie Th r 1 95 Ph e A a I Ie A a Hi s O31n Le u Le u G31u Ser Hi s Ph e ValI As p 1 65 Pr a G31u G31y As p A a 245 Ly s Le u Ly s G31y G31u 70 G31u Le u Ar g Tyr Ar g 150 Ly s vb t Ly s ValI A a 230 Ser ValI ValI Tyr As n G31y O31n As n Ar g G31y 135 Thr G31y Ar g A a ValI 215 ValI ValI (31u G31y As p 40 Pr a Ar g A a Ar g Ar g 120 Le u Pr a vb t ValI As p 200 Thr A a As p G31y A a li1e Pr a Ser Hi s Le u 1 05 Ph e ValI Le u Ar g Ar g 1 85 Ser ValI I Ie G31y G31y 265 ValI As p G31y G31y 75 As p Ph e As n G31y ValI 1 55 ValI Ar g As n ValI O31u 235 li1e Le u As p Ser Tr p Ly s A a Pr a Thr Ar g 140 Ar g ValI Ser Ly s A a 220 A a As p I Ie Pr a ValI Pr a A a As p Ly s Ser 125 O31u Le u A a ValI G31y 205 O31u vb t Ar g ValI A a li1e O31u Pr a As p Pr a 110 A a Thr As p As n O31u 1 Hi s G31y Ly s ValI ValI 270 As p A a Pr a Le u Ser Thr Le u Le u A a ValI 1 Ser ValI As p vb t ValI 255 ValI Ph e Ph e Le u Thr Ly s O31u As p I Ie I Ie 1 As n ValI A a O31u O31u 240 ValI Ser <210O> 113 <21 1> 939 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> 101). (916) <223> FRXA02326 <400> 113 ccaggcggac agt tgt ccaa cct gcgt gca caggccaccg cact gggcct t gcggat cgt Page 152 Gene Sequence 24 acgcaagc cgttaatgag -2-06. txt ttcgaactca tcgaagacaa ct acc Thr ct c Leu aag Lys ggt Gy gaa Gu gag Gu ct c Leu cgt Ar g t ac Tyr cgc Ar g 150 aag Lys at g Net aag Lys gtt Val gca Al a 230 tct Ser gt c Val ggt Gy gac Asp cct Pr o cgc Ar g gcg Al a cgc Ar g cgc Ar g 120 ct g Leu cca Pr o at g et gt g Val gat Asp 200 acc Thr gca Al a gac Asp cca Pr o ggt Gy cca Pr o ggt Gy gaa G u ct c Leu ct g Leu ggc Gy gaa G u ctt Leu aat Asn 170 gac Asp t cc Ser act Thr at c l Ie aaa Lys 250 t cc Ser gat Asp tct Ser tgg Tr p aag Lys gct Al a ccg Pr o acc Thr cgc Ar g 140 cgc Ar g gt g Val t cc Ser aag Lys gct Al a 220 gct Al a gat Asp aag Lys cca Pr o gt c Val 45 cca Pr o gca Al a gat Asp aag Lys tct Ser 125 gag G u ct g Leu gcc Al a gtt Val ggc Gy 205 gaa G u at g Net cgc Ar g gtt Val gca Al a at c SIle gag G u cct Pr o gat Asp cca Pr o 110 gcg Al a act Thr gat Asp aac Asn gag G u 190 cat Hi s ggt Gy aag Lys gtt Val gtt Val 15 gac Asp gcg Al a cca Pr o ct g Leu t cc Ser 95 acc Thr ct g Leu ttg Leu gcg Al a gt c Val 175 tct Ser gtt Val gat Asp at g Net gt g Val 255 ggc Gy ttt Phe ttc Phe ct g Leu acg Thr 80 aag Lys gaa G u gat Asp at c SIle at c l Ie 160 aac Asn gt c Val gct Al a gag G u gaa G u 240 gtt Val at g let 1 gac Asp gct Al a ct g Leu cgc Ar g gaa G u gaa Gu gag G u gat Asp cgc Ar g 145 tct Ser ggc Gy acc Thr gca Al a gt c Val 225 gca Al a cct Pr o ct c Leu gcc Al a cgc Ar g acc Thr gtt Val cgt Ar g ttc Phe cgt Ar g 130 ct g Leu gag G u cag G n gca Al a cca Pr o 210 aag Lys aca Thr gct Al a gca Al a gat Asp ggc Gy cgc Ar g cct Pr o cgc Ar g ct c Leu 115 gaa G u cca Pr o cca Pr o at c SIle acc Thr 195 ttc Phe gct Al a at c SIle gca Al a ct c Leu cca Pr o gag Gu gca Al a gag Gu aat Asn 100 gag G u ttc Phe gat Asp gac Asp cgc Ar g 180 gca Al a gct Al a gga Gy act Thr acg Thr 260 cac Hi s caa G n ctt Leu ctg Leu gaa G u agc Ser cac Hi s ttc Phe gtg Val gat Asp 165 cca Pr o gaa G u ggt Gy gat Asp gct Al a 245 aag Lys ctg gga cgc cca Leu Gy Arg Pro Page 153 Gene Sequence 24- 2- 06. txt gtg gaa ggt ggc gac ttg atc gtc gtc gtt tcc taaacctttc tgtaaaaagc 936 Val G u Gy y Asp Leu Ile Val Val Val Ser 265 270 ccc 939 <210> 114 <211> 272 <212> PRT <213> Corynebacter i um gl ut am cum <400> 114 IVet Leu y Arg Pro Thr Lys Val Thr Pro Ser Ser Lys Val Val y 1 5 10 Asp Leu Ala Leu His Leu Val G y Ala G y Val Asp Pro Ala Asp Phe 25 Ala Ala Asp Pro G n Lys Tyr Asp lie Pro Asp Ser Val I e Ala Phe 40 Leu Arg G y G u Leu G y Asn Pro Pro G y G y Trp Pro G u Pro Leu 55 Arg Thr Arg Al a Leu G u G y Arg Ser G u G y Lys Al a Pro Leu Thr 70 75 Su Val Pro G u u G u n Ala His Leu Asp Ala Asp Asp Ser Lys 90 G u Arg Arg Asn Ser Leu Asn Arg Leu Leu Phe Pro Lys Pro Thr G u 100 105 110 G u Phe Leu G u His Arg Arg Arg Phe G y Asn Thr Ser Ala Leu Asp 115 120 125 Asp Arg G u Phe Phe Tyr y Leu Val G u y Arg G u Thr Leu Ile 130 135 140 Arg Leu Pro Asp Val Arg Thr Pro Leu Leu Val Arg Leu Asp Ala lie 145 150 155 160 Ser G u Pro Asp Asp Lys y IVet Arg Asn Val Val Al a Asn Val Asn 165 170 175 Sy n Ile Arg Pro IVet Arg Val Arg Asp Arg Ser Val u Ser Val 180 185 190 Thr Ala Thr Ala G u Lys Ala Asp Ser Ser Asn Lys G y His Val Ala 195 200 205 Ala Pro Phe Ala y Val Val Thr Val Thr Val Ala G u y Asp G u 210 215 220 Val Lys Al a y Asp Ala Val Ala lle Ile G u Al a Iet Lys IVet u 225 230 235 240 Ala Thr lie Thr Ala Ser Val Asp y Lys Ile Asp Arg Val Val Val 245 250 255 Pro Ala Ala Thr Lys Val G u y y Asp Leu lie Val Val Val Ser 260 265 270 Page 154 Gene Sequence 24-2-06.txt <210> 115 <211> 1083 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1060) <223> RXN02327 <400> 115 accgctgaag cagct t ggcc cagccgcgtt tgctcgtgat ggcagttact gataccacct tccgcgatgc acaccagtct ctccgtgagc aggacgcact gt c Val act Thr gt g Val ct g Leu cgc Ar g ttt Phe gac Asp ct g Leu gat Asp aag Lys 150 aag Lys acc Thr gac Asp t ca Ser gag G u at g let gag G u acc Thr aag Lys ctt Leu acc Thr 120 tct Ser gca Al a at g Met ct g Leu gcg Al a 200 gca Al a ttg Leu ttc Phe at g let gga Gy gct Al a gac Asp acc Thr cca Pr o gag Su ggt Sy 170 cgt Ar g ggc G y ct g Leu t cc Ser ct c Leu ccg Pr o tac Ty r 75 gcc Al a gt c Val gcg Al a aat Asn at c SIle 155 ct g Leu gaa G u cag G n aag Lys gt g Val ttt Phe aat Asn acc Thr agc Ser t cc Ser gt a Val gaa G u 140 gt c Val ctt Leu ttc Phe ct g Leu cct Pr o gag G u gag G u 45 gt a Val ccg Pr o t cc Ser cag G n gcc Al a 125 aag Lys aag Lys cgc Ar g gat Asp gca Al a 205 gcg Al a gcc Al a gat Asp aac Asn t ac Tyr ggc Gy at g Met 110 gag G u ct c Leu tct Ser cca Pr o ct g Leu 190 acc Thr gca Al a 15 tgg Tr p ccg Pr o at t SIle cca Pr o gt g Val 95 cgt Ar g gt g Val t ac Tyr ggc G y gct Al a 175 cca Pr o t ac Tyr gag G u ggc G y tgg Tr p cag G n gac Asp 80 gac Asp cca Pr o gct Al a acc Thr gct Al a 160 gcg Al a gt g Val ttt Phe ttg Leu 1 gcc Al a ggc G y gac Asp at g let t cc Ser at c SIle gca Al a at g Met ct g Leu 145 cac Hi s gt a Val cac Hi s gct Al a gt c Val gcg Al a agg Ar g ct g Leu gt c Val ttc Phe at c l Ie gct Al a 130 gat Asp at c SIle acc Thr gt g Val gca Al a acc Thr ct c Leu ctt Leu tgc Cys cgc Ar g gac Asp 115 t at Tyr t ac Tyr ttg Leu aag Lys cac Hi s 195 aag Lys t ac Tyr gac Asp cgc Ar g cgc Ar g at c SIle 100 gca Al a tct Ser t ac Tyr gcc Al a ct g Leu 180 acc Thr ctt gcg acc Leu Al a Thr cga Ar g ctg Leu gat Asp gag G u ggc Gy gcg Al a ttc Phe gtc Val ggt G y ct a Leu att SIe 165 gtc Val cac Hi s gca gct caa gct Ala Ala G n Ala 210 Page 155 ggt gca gat gct gtt gac ggt gct t Sy Ala Asp Ala Val Asp y Ala S 215 220 ctc cca agc cat ccc ttg tct gcc a Leu Pro Ser His Pro Leu Ser Ala I 230 235 cgt cgc gat acc ggt ttg agc ctc g Arg Arg Asp Thr y Leu Ser Leu C 250 tac tgg gaa gca gtg cgc gga ctg t Tyr Trp G u Ala Val Arg y Leu T 265 2 cca ggc cca acc ggt cgc gtc tac c Pro y Pro Thr G y Arg Val Tyr P 280 285 ttg tcc aac ctg cgt gca cag gcc a Leu Ser Asn Leu Arg Al a n Ala T 295 300 ttc gaa ctc atc gaa gac aac tac g Phe G u Leu Ile G u Asp Asn Tyr A 310 315 caa <210> 116 <211> 320 <212> PRT <213> Corynebacter i um gl ut ai cum Sequence 24-2-06.txt cc ggc acc act gtc tgg cac cac 787 ier G y Thr Thr Val Trp His His 225 tt gtt gct gca ttc gcg cac acc 835 le Val Ala Ala Phe Ala His Thr 240 245 lag gct gtt tct gac ctc gag ccg 883 Su Ala Val Ser Asp Leu G u Pro 255 260 ac ctg cca ttt gag tct gga acc 931 yr Leu Pro Phe u Ser G y Thr 70 275 gc cac gaa atc cca ggc gga cag 979 ,rg His u lie Pro Gy Gy On 290 cc gca ctg ggc ctt gcg gat cgt 1027 hr Ala Leu Gy Leu Ala Asp Arg 305 ca agc cgt taatgagatg ctgggacgcc 1080 l a Ser Arg 320 1083 <400> 116 Leu Leu Al a 1 Al a Gy Asp et Ser SIle Al a bet Leu 145 Al a Thr Leu Leu Cys Ar g Asp 115 Tyr Tyr Thr Arg 5 Lys Leu Tyr Asp Asp u Arg G y Arg Ala Ile Phe 100 Al a Val Ser G y Tyr Leu Val Arg Ser Phe Pr o Al a Ar g Asn Val Al a G u Leu 135 Met Leu 25 Ar g Al a Val G u Asn 105 Asn Asp G u Al a Leu I 10 Leu Ser Phe Leu I Met Pro G y Tyr Al a Al a 90 Asp Val Thr Al a Pro Asn G u lie 155 Page 156 Pr o G u G u Val Pr o Ser G n Al a 125 Lys Lys Al a Al a Asp Asn Tyr Gy Met 110 G u Leu Ser Al a Tr p Pr o SIe Pr o Val Ar g Val Tyr Gy Hi s Val Hi s Al a Thr 225 Al a Ser Phe SIle Gy 305 Leu Lys Hi s 195 Al a Tr p Al a Leu Ser 275 Gy Al a SIle 165 Val Hi s Al a Hi s Thr 245 Pr o Thr G n Ar g Asp Al a Thr Al a 215 Pr o Ar g Tr p Gy Ser 295 G u Gene Sequence 24-2-06.txt IVet Al a G y Leu Leu Arg Pro 170 Leu Arg Arg G u Phe Asp Leu 185 190 Al a G y y G n Leu Al a Thr 200 205 Asp Ala Val Asp Gy Ala Ser 220 Ser His Pro Leu Ser Ala lie 235 Asp Thr y Leu Ser Leu G u 250 G u Ala Val Arg y Leu Tyr 265 270 Pro Thr y Arg Val Tyr Arg 280 285 Asn Leu Arg Ala G n Ala Thr 300 Leu Ile G u Asp Asn Tyr Al a 315 Al a 175 Pr o Tyr Gy Val Al a 255 Leu Hi s Al a Ser <210> 117 <211> 1083 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1060) <223> FRXA02327 <400> 117 accgctgaag cagcttggcc cagccgcgtt tgc ggcagt t act gataccacct tccgcgatgc aca gt c Val act Thr gt g Val ct g Leu cgc t ca Ser gag G u at g Net gag Geu acc gca Al a ttg Leu ttc Phe at g Net gga aag Lys gt g Val ttt Phe aat Asn acc cct Pr o gag G u gag G u 45 gt a Val ccg gcg Al a gcc Al a gat Asp aac Asn t ac t cgtgat ctccgtgagc aggacgcact iccagtct ttg ctt gcg acc cga Leu Leu Ala Thr Arg 1 gca gag gcc gtc gca aag ctg Ala G u Ala Val Ala Lys Leu 15 tgg ggc ggc gcg acc tac gat Trp Gy y Ala Thr Tyr Asp ccg tgg gac agg ctc gac gag Pro Trp Asp Arg Leu Asp G u att cag atg ctg ctt cgc ggc Ile G n Iet Leu Leu Arg y cca gac tcc gtc tgc cgc gcg Page 157 Ar g tt t Ph e g ac As p ct g Le u g at As p aag Ly s 1 50 aag Ly s ac c Thr g ac As p ggt G31y ct c Le u 230 cgt Ar g t ac Ty r cca Pr o tt g Le u Asn Thr Val G31y Tyr Thr 75 ene Sequenc Pro Tyr Pro e 24-2-06.txt aag Ly s ct t Le u ac c Th r 120 t ct Ser gca A a at g vb t ct g Le u gcg A a 200 g at As p ag c Ser g at As p g aa (31u cca Pr o 280 aac As n gct A a g ac As p ac c Th r cca Pr o gag (31u ggt G31y 1 70 cgt Ar g ggC G31y gt t ValI CCC Pr a ggt G31y 250 gt g ValI ggt G31y cgt Ar g gcc A a gt c ValI gcg A a aat As n at c SI e 155 ct g Le u gaa (31u cag O31n gac As p t t g Le u 235 t t g Le u cgc Ar g cgc Ar g gca A a gac As p 315 ag c Ser t cc Ser gt a ValI g aa G31u 140 gt c ValI ct t Le u tt c Ph e ct g Le u ggt G31y 220 t ct Ser ag c Ser gga G31y gt c ValI c ag O31n 300 t cc Ser c ag O31n gcc A a 125 aag Ly s aag Ly s cgc Ar g g at As p gca A a 205 gct A a gcc A a ct c Le u ct g Le u t ac Ty r 285 gcc A a ggC G31y at g vb t 110 gag O31u ct c Le u t ct Ser cca Pr a ct g Le u 1 90 ac c Th r t cc Ser at t I Ie gag O31u t ac Ty r 270 cgc Ar g ac c Th r gt g ValI 95 cgt Ar g gt g ValI t ac Ty r ggC G31y gct A a 1 75 cca Pr a t ac Ty r ggC G31y gt t ValI gct A a 255 ct g Le u c ac Hi s gca A a As p 80 g ac As p cca Pr a gct A a ac c Th r gct A a 1 gcg A a gt g ValI tt t Ph e ac c Th r gct A a 240 gt t ValI cca Pr a g aa O31u ct g Le u cgt Ar g 320 Ser Val at c I Ie gca A a at g vb t ct g Le u 145 c ac Hi s gt a ValI c ac Hi s gct A a act Th r 225 gca A a t ct Ser tt t Ph e at c G31y 305 ttc cgc Phe Ar g at c gac I I e As p 115 gct t at A a Ty r 130 gat t ac Asp Tyr at c tt g I I e Leu acc aag Thr Lys gt g cac Val Hi s 1 gca gct A a A a 210 gt c t gg Val Tr p ttc gcg Phe A a gac ct C Asp Leu gag t ct 03 u Ser 275 cca ggc Pro G31y 290 ctt gcg Leu A a at c I Ie 1 00 gca A a t ct Ser t ac Ty r gcc A a ct g Le u 1 ac c Th r c aa O31n c ac Hi s c ac Hi s gag O31u 260 gga G31y gga G31y g at As p 403 451 499 547 595 643 691 739 787 835 883 931 979 1 027 Cy s Ar g Ala t tc gaa ct c at c gaa Phe 03 u Leu I I e 03 u 310 c aa <210> 118 <21 1> 320 aac t ac gca agc Asn Tyr Al aSer taatgagatg ctgggacgcc 1080 1 083 Page 158 Gene Sequence 24-2-06.txt <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 118 Leu Leu Al a 1 Al a Gy Asp et Ser SIle Al a bet Leu 145 Hi s Val Hi s Al a Thr 225 Al a Ser Phe SIle Gy 305 Al a Thr Leu Leu Cys Ar g Asp 115 Tyr Tyr Leu Lys Hi s 195 Al a Tr p Al a Leu Ser 275 Gy Al a Thr Arg 5 Lys Leu Tyr Asp Asp u Arg G y Arg Ala Ile Phe 100 Al a Val Ser G y Tyr Leu Ala I e 165 Leu Val 180 Thr His G n Al a Hi s Hi s Hi s Thr 245 G u Pro 260 G y Thr G y G n Asp Arg Pr o Al a Ar g Asn Val Al a G u Leu 135 let Asp Al a Thr Al a 215 Pr o Ar g Tr p Gy Ser 295 G u Val Arg Ser Phe Leu 25 Ar g Al a Val G u Asn 105 Asn Asp G u Al a Ar g 185 Gy Al a Hi s Thr Al a 265 Thr Leu SIle Al a 10 Leu Phe let Gy Al a 90 Asp Thr Pr o G u Gy 170 Ar g Gy Val Pr o Gy 250 Val Gy Ar g G u Leu Ser Leu Pr o Tyr 75 Al a Val Al a Asn SIle 155 Leu G u G n Asp Leu 235 Leu Ar g Ar g Al a Asp 315 Pr o G u G u Val Pr o Ser G n Al a 125 Lys Lys Ar g Asp Al a 205 Al a Al a Leu Leu Tyr 285 Al a Tyr Al a Al a Asp Asn Tyr Gy vet 110 G u Leu Ser Pr o Leu 190 Thr Ser l Ie G u Tyr 270 Ar g Thr Al a Al a Tr p Pr o SIle Pr o Val Ar g Val Tyr Gy Al a 175 Pr o Tyr Gy Val Al a 255 Leu Hi s Al a Ser G u Gy Tr p G n Asp Asp Pr o Al a Thr Al a 160 Al a Val Phe Thr Al a 240 Val Pr o G u Leu Ar g 320 Page 159 Gene Sequence 24-2-06.txt <210> 119 <211> 1719 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1696) <223> RXN02328 <400> 119 gaagtcgtgc aggtcagggg agtgttgccc gaaaacattg gtttgattgg gggaatcggg ggttacgata ctaggacgca agaggaaaac aaaaaccgat ctt Leu aca Thr ggc Gy gcc Al a tct Ser aag Lys aaa Lys cag G n acc Thr gcc Al a 150 aaa Lys at c SIle gtt Val ggt Sy t ca Ser at c I e gt a Val gct Al a t ac Tyr gat Asp gcc Al a 120 gag Su aag Lys at c SIle gt g Val t ca Ser 200 ttg Leu ctt Leu gt c Val at t SIle gaa Gu gac Asp at t SIle gag Gu ctt Leu gct Al a gag G u 170 gca Al a gat Asp tt g Leu cca Pr o cgt Ar g tac Tyr gct Al a 75 at c SIle tac Tyr tgt Cys gat Asp ggt G y 155 at c SIle gtt Val gag G u aaa Lys gca Al a gct Al a ccc Pr o gt c Val gat Asp ccg Pr o gcg Al a ct c Leu 140 ct g Leu gtt Val gcc Al a ctt Leu gga G y ttc Phe ttc Phe 45 cgt Ar g cgc Ar g gaa Gu gga Gy gaa Gu 125 acc Thr cca Pr o aaa Lys ggt G y cgc Ar g 205 at a SIle aaa Lys cgt Ar g gaa Gu at t SIle at t SIle t ac Tyr 110 aac Asn ggt G y gtt Val agc Ser ggt G y 190 aaa Lys at t I e 15 aag Lys gca Al a gat Asp ggt Gy at c SIle 95 ggc Gy ggc G y gat Asp ttg Leu gct Al a 175 ggc G y tta Leu act Thr at c SIle gca Al a cgg Ar g acc Thr 80 ggt Gy ttc Phe at t SIle aag Lys gcg Al a 160 gaa Gu gga G y gca Al a gt g Val 1 ct a Leu ttg Leu ct c Leu gga Gy gaa Gu gca Al a ct g Leu act Thr tct Ser 145 gaa Gu ggc G y cgc Ar g aca Thr gt g Val gt a Val gaa Gu t ca Ser ggc Gy gct Al a tct Ser ttt Phe 130 cgc Ar g t cc Ser cag G n ggt G y gaa G u 210 tcg Ser gca Al a acc Thr ttc Phe t ca Ser aaa Lys gaa G u 115 at t SIle gcg Al a acc Thr act Thr at g Met 195 gca Al a act Thr aac Asn ggt Gy cac Hi s cca Pr o aaa Lys 100 aat Asn ggc G y gt a Val ccg Pr o t ac Tyr 180 cgt Ar g tct Ser act gct at c Thr Ala li e acc Thr cac Hi s cgc Ar g gca Al a cgc Ar g gtc Val gtt Val gcc Al a cca Pr o acc Thr agc Ser 165 ccc Pr o ttt Phe cgt Ar g Page 160 Gene Sequence 24-2-06.txt gaa G u gt g Val 230 gga G y cac Hi s ct g Leu ggt Sy aac Asn 310 gt g Val ttg Leu at c SIle cca Pr o t ca Ser 390 ggc G y cgt Ar g gct Al a gcg Al a ttc Phe 470 gaa G u aac Asn gtt Val aaa Lys gat Asp 280 cag G n gt c Val gaa G u gct Al a acc Thr 360 aac Asn ggc G y at c SIle t cc Ser ttc Phe 440 ct g Leu gcc Al a gct Al a cag G n cac Hi s 250 gt c Val at t SIle gcg Al a at c SIle gt c Val 330 gca Al a ggt G y ttc Phe gct Al a gca Al a 410 ttt Phe gt g Val gaa G u cac Hi s tt c Phe cat Hi s 235 ctt Leu gaa G u tgt Cys gga G y gaa G u 315 acc Thr acc Thr gca Al a cgc Ar g ggc G y 395 cac Hi s gaa G u tct Ser gag G u ccg Pr o 475 ggc G y 220 at t SIle t at Tyr at t SIle gcg Al a acc Thr 300 at g Net gag G u ttg Leu gca Al a cca Pr o 380 gtt Val ttt Phe act Thr ggt G y gac Asp 460 cac Hi s gat ggc gcg gta Asp y Ala Val gaa G u gaa G u gcg Al a gat Asp 285 gt g Val aac Asn gt g Val aag Lys ct g Leu 365 gat Asp cgt Ar g gac Asp gct Al a gtt Val 445 ttc Phe ct c Leu gt g Val cgt Ar g cca Pr o 270 gca Al a gaa G u cca Pr o gac Asp gaa G u 350 cag G n acc Thr ctt Leu t cc Ser gtt Val 430 gca Al a act Thr ctt Leu cag G n gac Asp 255 gca Al a gt a Val ttc Phe cgt Ar g ct g Leu 335 ttg Leu tgc Cys gga G y gac Asp at g let 415 gct Al a acc Thr t cc Ser cag G n at c SIle 240 tgc Cys cag G n aag Lys ttg Leu at c SIle 320 gt g Val ggt G y cgc Ar g act Thr ggt G y 400 ct g Leu cgt Ar g aac Asn aag Lys gct Al a 480 t at Tyr 225 ctt Leu t ca Ser cat Hi s ttc Phe gt c Val 305 cag G n aag Lys ct g Leu at c SIle at c SIle 385 gca Al a gt g Val gca Al a at t SIle cgc Ar g 465 cca Pr o gtc gaa cgt gct Val G u Arg Ala ggc G y ct g Leu ttg Leu tgc Cys 290 gat Asp gtt Val gcg Al a acc Thr acc Thr 370 acc Thr gct Al a aaa Lys cag G n ggt G y 450 at c SIle cct Pr o gat Asp cag G n gat Asp 275 cgc Ar g gaa G u gag G u cag G n caa G n 355 acg Thr gcg Al a cag G n at g Met cgc Ar g 435 ttc Phe gcc Al a gct Al a cac Hi s cgt Ar g 260 cca Pr o t cc Ser aag Lys cac Hi s at g Net 340 gat Asp gaa G u t ac Tyr ct c Leu acc Thr 420 gcg Al a ttg Leu acc Thr gat Asp act Thr 245 cgt Ar g gaa G u at t SIe ggc G y acc Thr 325 cgc Ar g aag Lys gat Asp cgc Ar g ggt G y 405 tgc Cys ttg Leu cgt Ar g gga G y gat Asp 485 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 Page 161 Gene Sequence 24- 2- 06. txt gag cag gga cgc atc ctg gat tac ttg gca gat gtc acc gtg aac G u n y Arg lie Leu Asp Tyr Leu Ala Asp Val Thr Val Asn 490 495 500 cct cat ggt gtg cgt cca aag gat gtt gca gct cct atc gat aag Pro His G y Val Arg Pro Lys Asp Val Ala Ala Pro IIe Asp Lys 505 510 515 cct aac atc aag gat ctg cca ctg cca cgc ggt t cc cgt gac cgc Pro Asn lie Lys Asp Leu Pro Leu Pro Arg Gy Ser Arg Asp Arg 520 525 530 tgaagcagct tggcccagcc gcg <210> 120 <211> 532 <212> PRT <213> Cor ynebact eri um gl ut am cum 1603 1651 1696 1719 <400> 120 Val Thr Al a I e Thr Leu Gy Gy Leu 1 Leu Leu Leu Gy G u Al a Leu Thr Ser 145 G u Gy Ar g Thr Tyr 225 Ser Al a Thr Phe Ser Lys G u 115 I le Al a Thr Thr vet 195 Al a G u 5 Hi s Ar g Al a Ar g Val Val Al a Pr o Thr Ser 165 Pr o Phe Ar g Al a Ser G u Thr Phe Al a Al a Leu Pr o 135 Al a Asn Phe Al a Al a 215 l le Thr Al a Al a Ser Leu Al a 105 Ar g Val Lys Asp Lys 185 Pr o Al a Pr o Leu 10 Leu Val l Ie G u Asp 90 l e G u Leu Al a G u 170 Al a Asp Al a G n Leu Pr o Ar g Tyr Al a 75 I le Tyr Cys Asp Gy 155 I le Val G u Phe Hi s 235 Lys Al a Al a Pr o Val Asp Pr o Al a Leu 140 Leu Val Al a Leu Gy 220 I le Gy Phe Phe Ar g Ar g G u Gy G u 125 Thr Pr o Lys Gy Ar g 205 Asp G u I e Lys Al a Asp Gy I e Gy Gy Asp Leu Al a 175 Gy Leu Al a G n Thr SIe Al a Ar g Thr Gy Phe SIe Lys Al a 160 G u Gy Al a Val SIe 240 Leu y Asp His Thr G y G u Val Val His Leu Tyr G u Arg Asp Cys Page 162 Gene Sequence 24-2-06.txt Ser Hi s Phe Val 305 G n Lys Leu SIle SIle 385 Al a Val Al a SIle Ar g 465 Pr o Val Pr o Ser G n Asp 275 Ar g G u G u G n G n 355 Thr Al a G n let Ar g 435 Phe Al a Al a Val Asp 515 Asp 245 Ar g G u SIle Gy Thr 325 Ar g Lys Asp Ar g Gy 405 Cys Leu Ar g Gy Asp 485 Lys Leu 250 Val u Ile Ala Hi s Leu Gy Asn 310 Val Leu SIle Pr o Ser 390 Gy Ar g Al a Al a Phe 470 G u Pr o Pr o G n Ar g Tyr 295 Hi s Thr Al a Lys Asn 375 Pr o G u Sy G u Leu 455 I le G n Hi s Asn Lys Asp 280 G n Val G u Al a Thr 360 Asn Sy SIle Ser Phe 440 Leu Al a Sy Sy SIle 520 Val 265 Ar g Sy Phe G u Sy 345 Hi s Sy Sy Thr Asp 425 Thr Ar g Asp Ar g Val 505 Lys SIle Al a SIle Val 330 Al a Sy Phe Al a Al a 410 Phe Val G u Hi s SIle 490 Ar g Asp Cys Sy G u 315 Thr Thr Al a Ar g Sy 395 Hi s G u Ser G u Pr o 475 Leu Pr o Leu Al a Thr 300 let G u Leu Al a Pr o 380 Val Phe Thr Sy Asp 460 Hi s Asp Lys Pr o Asp 285 Val Asn Val Lys Leu 365 Asp Ar g Asp Al a Val 445 Phe Leu Tyr Asp Leu 525 Pr o 270 Al a G u Pr o Asp G u 350 G n Thr Leu Ser Val 430 Al a Thr Leu Leu Val 510 Pr o 255 Al a Val Phe Ar g Leu 335 Leu Cys Sy Asp et 415 Al a Thr Ser G n Al a 495 Al a Ar g G n Lys Leu SIe 320 Val Sy Ar g Thr Sy 400 Leu Ar g Asn Lys Al a 480 Asp Al a Sy <210> 121 <211> 1406 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (1)..(1383) <223> FRXA02328 <400> 121 Page 163 Gene Sequence 24-2-06.txt gct Al a 1 t ac Ty r gat Asp gcc Al a gag G u aag Lys at c SIle gt g Val t ca Ser gaa G u 145 aac Asn gtt Val aaa Lys gat Asp cag G n 225 gt c Val gaa G u t ct gaa gct Ser G u Al a ct g gac at c Leu Asp Ile gcc att tac Al a I le Tyr cgc gag tgt Arg u Cys gtt ctt gat Val Leu Asp aag gct ggt Lys Al a G y gat gag at c Asp G u Ile 100 aag gca gtt Lys Al a Val 115 cct gat gag Pro Asp G u 130 gcg gct ttc Al a Al a Phe cct cag cat Pro G n Hi s gt a cac ct t Val Hi s Leu 180 gtt gtc gaa Val Val G u 195 cgc att tgt Arg lie Cys 210 ggc gcg gga G y Al a G y ttc atc gaa Phe Ile G u gaa gt c acc G u Val Thr gtc cgc att ggt Val Arg I e G y 5 gat gaa att at c Asp G u Ile Il e ccg gga tac ggc Pro y Tyr y 40 gcg gaa aac ggc Ala G u Asn G y 55 ctc acc ggt gat Leu Thr G y Asp 70 ctg cca gtt ttg Leu Pro Val Leu gtt aaa agc gct Val Lys Ser Al a gcc ggt ggt ggc Ala y G y y 120 ctt cgc aaa tta Leu Arg Lys Leu 135 ggc gat ggc gcg Sy Asp y Ala 150 att gaa gtg cag Ile u Val n 165 tat gaa cgt gac Tyr G u Arg Asp att gcg cca gca Ile Ala Pro Ala 200 gcg gat gca gta Al a Asp Al a Val 215 acc gtg gaa ttc Thr Val G u Phe 230 atg aac cca cgt IVet Asn Pro Arg 245 gag gtg gac ctg G u Val Asp Leu acc gaa ggc Thr G u G y tca cca gtc aag gcg Ser Pro Val Lys Ala ggt Gy 25 ttc Phe at t SIle aag Lys gcg Al a gaa Gu 105 gga Gy gca Al a gt a Val at c SIle tgc Cys 185 cag G n aag Lys ttg Leu at c SIle gt g Val 265 gca Al a ct g Leu act Thr tct Ser gaa G u 90 ggc Gy cgc Ar g aca Thr t at Tyr ctt Leu 170 t ca Ser cat Hi s ttc Phe gt c Val cag G n 250 aag Lys gct Al a tct Ser ttt Phe cgc Ar g 75 t cc Ser cag G n ggt Sy gaa G u gt c Val 155 ggc Sy ct g Leu ttg Leu tgc Cys gat Asp 235 gtt Val gcg Al a aaa Lys gaa G u at t I e gcg Al a acc Thr act Thr at g bet gca Al a 140 gaa G u gat Asp cag G n gat Asp cgc Ar g 220 gaa G u gag G u cag G n aaa Lys aat Asn ggc G y gt a Val ccg Pr o t ac Tyr cgt Ar g 125 tct Ser cgt Ar g cac Hi s cgt Ar g cca Pr o 205 t cc Ser aag Lys cac Hi s at g bet gtt Val gcc Al a cca Pr o acc Thr agc Ser ccc Pr o 110 ttt Phe cgt Ar g gct Al a act Thr cgt Ar g 190 gaa G u at t SIle ggc G y acc Thr cgc Ar g 270 aaa Lys cag G n acc Thr gcc Al a aaa Lys at c SIle gtt Val gaa G u gt g Val gga Sy 175 cac Hi s ct g Leu ggt G y aac Asn gt g Val 255 ttg Leu gca Al a ctt Leu cca Pr o gcg Al a aac Asn ttt Phe gct Al a gct Al a att SIe 160 gaa G u caa G n cgt Ar g t ac Tyr cac Hi s 240 act Thr gct Al a Page 164 Gene Sequence 24-2-06.txt gct ggt gca acc ttg aag gaa ttg ggt Ala Gy Ala Thr Leu Lys u Leu Gy ctg acc caa gat aag atc aag Leu Thr G n Asp Lys Ile Lys 285 275 acc cac ggt gca gca ctg cag Thr His G y Ala Ala Leu G n 290 295 aac ggc ttc cgc cca gat acc Asn y Phe Arg Pro Asp Thr 305 310 ggc gga gct ggc gtt cgt ctt G y G y Ala G y Val Arg Leu 325 atc acc gca cac ttt gac tcc Ile Thr Ala His Phe Asp Ser 340 tcc gac ttt gaa act gct gtt Ser Asp Phe G u Thr Al a Val 355 ttc acc gtg tct ggt gtt gca Phe Thr Val Ser G y Val Al a 370 375 ctg cgg gaa gag gac ttc act Leu Arg G u G u Asp Phe Thr 385 390 gcc gat cac ccg cac ctc ctt Ala Asp His Pro His Leu Leu 405 gga cgc atc ctg gat tac ttg Sy Arg lie Leu Asp Tyr Leu 420 ggt gtg cgt cca aag gat gtt Sy Val Arg Pro Lys Asp Val 435 atc aag gat ctg cca ctg cca lie Lys Asp Leu Pro Leu Pro 450 455 tggcccagcc gcg <210> 122 <211> 461 <212> PRT <213> Corynebacter i um gl ut a <400> 122 Ala Ser u Ala Val Arg Ile 1 5 Tyr Leu Asp Ile Asp Ou Ile Asp Ala lie Tyr Pro y Tyr Ala Arg G u Cys Ala G u Asn 55 280 tgc Cys gga Gy gac Asp at g Net gct Al a 360 acc Thr t cc Ser cag G n gca Al a gca Al a 440 cgc Ar g act Thr ggt Gy ct g Leu 345 cgt Ar g aac Asn aag Lys gct Al a gat Asp 425 gct Al a at c SIle at c SIle gca Al a 330 gt g Val gca Al a at t SIle cgc Ar g cca Pr o 410 gt c Val cct Pr o acc Thr acc Thr 315 gct Al a aaa Lys cag G n ggt Gy at c SIle 395 cct Pr o acc Thr at c SIle acg Thr 300 gcg Al a cag G n at g Net cgc Ar g ttc Phe 380 gcc Al a gct Al a gt g Val gat Asp gaa G u t ac Tyr ct c Leu acc Thr gcg Al a 365 ttg Leu acc Thr gat Asp aac Asn aag Lys 445 cca Pr o t ca Ser ggc Gy 335 cgt Ar g gct Al a gcg Al a ttc Phe gag G u 415 cct Pr o cct Pr o aac Asn cca Pr o 320 gaa G u ggt Gy gag G u ttg Leu at t SIe 400 cag G n cat Hi s aac Asn 864 912 960 1008 1056 1104 1152 1200 1248 1296 1344 1393 1406 cgc ggt tcc cgt Arg y Ser Arg gac cgc Asp Arg 460 tgaagcagct ri cum Sy Thr u y Ser Pro Val Lys Ala 10 lie y Ala Ala Lys Lys Val Lys Ala 25 G y Phe Leu Ser G u Asn Al a G n Leu 40 Gy Ile Thr Phe lie Gy Pro Thr Pro Page 165 Gene Sequence 24-2-06.txt G u Val Leu Asp Leu Thr y Asp Lys Ser Lys SIle Val Ser G u 145 Asn Val Lys Asp G n 225 Val G u Al a Thr Asn 305 Sy SIle Ser Phe Leu 385 Al a Al a G u Al a 115 Asp Al a G n Hi s Val 195 I le Al a SIle Val Al a 275 Sy Phe Al a Al a Phe 355 Val G u Hi s Leu Val Al a Leu Sy SIle 165 Tyr SIle Al a Thr bet 245 G u Leu Al a Pr o Val 325 Phe Thr Sy Asp Hi s 405 70 Pr o Lys Sy Ar g Asp 150 G u G u Al a Asp Val 230 Asn Val Lys Leu Asp 310 Ar g Asp Al a Val Phe 390 Leu Val Ser Sy Lys 135 Sy Val Ar g Pr o Al a 215 G u Pr o Asp G u G n 295 Thr Leu Ser Val Al a 375 Thr Leu Leu Al a Sy 120 Leu Al a G n Asp Al a 200 Val Phe Ar g Leu Leu 280 Cys Sy Asp Met Al a 360 Thr Ser G n Al a G u 105 Gy Al a Val SIle Cys 185 G n Lys Leu SIle Val 265 Gy Ar g Thr Gy Leu 345 Ar g Asn Lys Al a G u 90 Gy Ar g Thr Tyr Leu 170 Ser Hi s Phe Val G n 250 Lys Leu SIle SIle Al a 330 Val Al a SIle Ar g Pr o 410 Ar g Ser G n Sy G u Val 155 Sy Leu Leu Cys Asp 235 Val Al a Thr Thr Thr 315 Al a Lys G n Sy SIle 395 Pr o Thr Thr let Al a 140 G u Asp G n Asp Ar g 220 G u G u G n G n Thr 300 Al a G n Met Ar g Phe 380 Al a Al a Pr o Tyr Ar g 125 Ser Ar g Hi s Ar g Pr o 205 Ser Lys Hi s Met Asp 285 G u Tyr Leu Thr Al a 365 Leu Thr Asp Ala Val Thr Ala Ala Lys I e Val G u Val Sy 175 Hi s Leu Sy Asn Val 255 Leu SIle Pr o Ser Sy 335 Ar g Al a Al a Phe G u 415 Asn Phe Al a Al a SIe 160 G u G n Ar g Tyr Hi s 240 Thr Al a Lys Asn Pr o 320 G u Sy G u Leu SIe 400 G n Sy Arg lie Leu Asp Tyr Leu Ala Asp Val Thr Val Asn Lys Pro His Page 166 ene Sequence 24- 2- 06. txt 420 425 430 Gy Val Arg Pro Lys Asp Val Al a Ala Pro lieAsp Lys Leu Pro Asn 435 440 445 Ilie Lys Asp Leu Pro Leu Pro Ar g 3 y Se r Ar g Asp Ar g 450 455 460 <210> 123 <211> 1347 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CDS <222> (1324) <223> RXN01048 <400> 123 agct acagat t tagct agt g t tt t tgt tcc agaaccct aa at gaggt tct accct taaca gagct tcccg caaaaacacc gat taacaag gct aaat gat at g acc at c gac ct g I'bt Thr I Ie Asp Leu c ag O31n gag O31u cgc Ar g gca Al a aac As n g at As p ct g Le u gt t ValI t ct Ser gag O31u 1 50 g at As p t cc Ser gga G31y ct t Le u aag Ly s gt c ValI gga G31y ag c Ser g at As p 120 ggt G31y gag O31u c ag O31n c aa O31n ct c Le u ct t Le u g at As p gt t ValI c ag O31n t tc Ph e g ac As p at c I Ie cgc Ar g ggc (31y aac As n t cc Ser gct Al a cca Pr o at t I Ie 75 gcc Al a gct Al a gct Al a aac As n ct c Le u 155 acc Thr ct c Le u at t I Ie t ac Tyr gag O31u t cc Ser t cc Ser ggc O31y tt g Le u tt g Le u 140 at c I Ie gct Al a cat Hi s t cc Ser cct Pr o gcg Al a ggc O31y ccc Pr o aag Ly s 110 gag O31u g ac As p cgt Ar g gt t ValI g aa O31u cgt Ar g gt t ValI ac c Thr gct Al a at g vb t at c I Ie at c I Ie t cc Ser g at As p 1 1 at c I Ie ccg Pr a gct Al a c ac Hi s gt t ValI gag O31u cct Pr a gca Al a gct Al a 145 at t I Ie tt c Ph e ct c Le u c ag O31n ac g Thr ct t Le u ggc O31y at c I Ie gcc Al a 130 cct Pr a cca Pr a gca Al a g ac As p t gt cys at t I Ie ct t Le u gct Al a 1 00 tt g Le u gcg Al a t gc cys at g vb t atc ctc gct gcg ctg cgc aac Ilie Leu Ala Ala Leu Arg Asn Page 167 Gene Sequence 24-2-06.txt 170 175 180 tcc ctg aag ctg ctg gat cgc aag atc gaa gac ctc aag att gtt att 691 Ser Leu Lys Leu Leu Asp Arg Lys Ile G u Asp Leu Lys Ile Val lie 185 190 195 tcc ggc gca ggc gca gcg ggc gtt gca gct gta gat atg ctg acc aac 739 Ser y Ala y Ala Al a y Val Ala Ala Val Asp IVet Leu Thr Asn 200 205 210 gct gga gca acc gac atc gtg gtt ctt gat tcc cga ggc atc atc cac 787 Al a y Ala Thr Asp ile Val Val Leu Asp Ser Arg G y lie lie His 215 220 225 gac agc cgt gag gat ctt tcc cca gtt aag gct gct ctt gca gag aag 835 Asp Ser Arg G u Asp Leu Ser Pro Val Lys Ala Ala Leu Ala G u Lys 230 235 240 245 acc aac cct cgt ggc atc agc ggt ggc atc aat gag gct t tc acc ggc 883 Thr Asn Pro Arg Gy lie Ser Gy Gy lie Asn u Ala Phe Thr y 250 255 260 gcg gac ctg ttc att ggc gtg tcc ggc ggc aac atc ggc gag gac gct 931 Ala Asp Leu Phe Ile G y Val Ser G y y Asn Ile G y G u Asp Ala 265 270 275 ctc aaa ctc atg gcc ccg gag cca atc ctg ttc acc ctg gcg aac cca 979 Leu Lys Leu IVet Ala Pro G u Pro lie Leu Phe Thr Leu Ala Asn Pro 280 285 290 acc cca gag atc gat cct gag ctg tct cag aag tac ggc gcc atc gtc 1027 Thr Pro u lie Asp Pro u Leu Ser n Lys Tyr y Ala lie Val 295 300 305 gcg acc ggg cgc tct gac ctg cct aac cag atc aac aac gtg ctg gcg 1075 Ala Thr y Arg Ser Asp Leu Pro Asn G n Ile Asn Asn Val Leu Ala 310 315 320 325 ttc cca gga att ttc gcc ggc gct ctc gca gcc aag gct aag aag atc 1123 Phe Pro y lie Phe Al a y Ala Leu Ala Ala Lys Ala Lys Lys Ile 330 335 340 acc ccc gag atg aag ctc gcc gct cag agg caa tcg cag aca tcg cag 1171 Thr Pro G u Iet Lys Leu Ala Ala G n Arg G n Ser G n Thr Ser G n 345 350 355 ctg agg acc tcg agg tcg gcc gca tcg tgc cta ccg ccc tgg atc ccc 1219 Leu Arg Thr Ser Arg Ser Ala Ala Ser Cys Leu Pro Pro Trp lie Pro 360 365 370 gcg ttg ccc caa cag tca agg cag ctg tcc agg ccg tcg cca aag cgc 1267 Ala Leu Pro n G n Ser Arg G n Leu Ser Arg Pro Ser Pro Lys Arg 375 380 385 aaa acg ctt aaa aat ttg ctt atc gac gcc tcc ctc ccc gtc gag gcg 1315 Lys Thr Leu Lys Asn Leu Leu Ile Asp Ala Ser Leu Pro Val G u Ala 390 395 400 405 cca ata ttt taagagcaaa cttgaggccc aca 1347 Pro Ile Phe <210> 124 <211> 408 <212> PRT <213> Cor ynebact eri um gl ut am cum Page 168 Gene Sequence 24-2-06.txt <400> 124 et 1 I e Pr o Al a Hi s Val G u Pr o Al a Al a 145 I le Al a Leu Asp Ar g 225 Al a G u I e Thr Tyr 305 Asn Lys Thr Ile Asp Phe G u Al a Leu Arg Asp G n Val Cys Thr G y Ile Leu G y Leu G y Lys Al a 100 Ile Val Leu 115 Al a I e Al a 130 Pro Arg Cys Pro Val IVet Ala Leu Arg 180 Lys Ile Val 195 IVet Leu Thr 210 G y lie lle Leu Al a G u Al a Phe Thr 260 Sy G u Asp 275 Leu Al a Asn 290 G y Al a lle Asn Val Leu Ala Lys Lys 340 Leu 5 Hi s bet G u Sy Sy G n Asp Pr o Phe Hi s 165 Asn SIle Asn Hi s Lys 245 Sy Al a Pr o Val Al a 325 I le G n Arg Ser Thr n Asn Leu Thr His u u 10 u Ar g Al a Asn 70 Asp Leu Val Ser G u 150 Asp Ser Ser Al a Asp 230 Thr Al a Leu Thr Al a 310 Phe Thr Sy Asp l Ie 55 Thr SIle Phe Hi s Phe 135 Val Asp Leu Sy Sy 215 Ser Asn Asp Lys Pr o 295 Thr Pr o Pr o Sy Leu 40 Lys Val Sy Ser Asp 120 Gy G u G n Lys Al a 200 Al a Ar g Pr o Leu Leu 280 G u Sy Sy G u Lys Ser G u Al a Pr o Ser 105 Val Al a Ar g Hi s Leu 185 Sy Thr G u Ar g Phe 265 bet SIle Ar g SIle Net 345 Leu Leu Asp Val G n 90 Phe Asp l Ie Ar g Sy 170 Leu Al a Asp Asp Sy 250 I le Al a Asp Ser Phe 330 Lys Ser Al a Pr o SIle 75 Al a Al a Al a Asn Leu 155 Thr Asp Al a SIle Leu 235 I le Sy Pr o Pr o Asp 315 Al a Leu SIle Tyr G u Ser Ser Sy Leu Leu 140 I le Al a Ar g Sy Val 220 Ser Ser Val G u G u 300 Leu a y Al a Ser Thr Val Asp Leu Leu Val 125 G u G u Val Lys Val 205 Val Pr o Sy Ser Pr o 285 Leu Pr o Al a Al a Thr Sy Ar g Thr Val Al a Thr SIle Leu SIle 175 G u Al a Asp Lys SIle 255 Sy Leu G n G n Al a 335 Ar g Ar g Val Thr Al a bet SIe SIe Ser Asp 160 Leu Asp Val Ser Al a 240 Asn Asn Phe Lys SIe 320 Al a G n Ser G n Thr Ser G n Leu Arg Thr Ser Arg Ser Ala Ala Ser Cys Leu Page 169 Gene Sequence 24-2-06.txt 355 360 365 Pro Pro Trp lie Pro Ala Leu Pro n n Ser Arg G n Leu Ser Arg 370 375 380 Pro Ser Pro Lys Arg Lys Thr Leu Lys Asn Leu Leu Ile Asp Ala Ser 385 390 395 400 Leu Pro Val G u Ala Pro Ile Phe 405 <210> 125 <211> 311 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> FRXA01048 <400> 125 cgc tct gac ttg cct aac cag atc aac aac gtg ctg gcg ttc cca gga 48 Arg Ser Asp Leu Pro Asn n Ile Asn Asn Val Leu Ala Phe Pro y 1 5 10 att ttc gcc ggc gct ctc gca gcc aag gct aag aag atc acc ccc gag 96 Ile Phe Ala y Ala Leu Ala Ala Lys Ala Lys Lys Ile Thr Pro G u 25 atg aag ctc gcc gct cag agg caa tcg cag aca tcg cag ctg agg acc 144 IVet Lys Leu Ala Ala G n Arg G n Ser G n Thr Ser G n Leu Arg Thr 40 tcg agg tcg gcc gca tcg tgc cta ccg ccc tgg atc ccc gcg ttg ccc 192 Ser Arg Ser Ala Ala Ser Cys Leu Pro Pro Trp lie Pro Ala Leu Pro 55 caa cag tca agg cag ctg tcc agg ccg tcg cca aag cgc aaa acg ctt 240 Sn G n Ser Arg G n Leu Ser Arg Pro Ser Pro Lys Arg Lys Thr Leu 70 75 aaa aat ttg ctt atc gac gcc tcc ctc ccc gtc gag gcg cca ata ttt 288 Lys Asn Leu Leu lie Asp Ala Ser Leu Pro Val G u Ala Pro Ile Phe 90 taagagcaaa cttgaggccc aca 311 <210> 126 <211> 96 <212> PRT <213> Cor ynebact er i um gl ut ari cum <400> 126 Arg Ser Asp Leu Pro Asn n Ile Asn Asn Val Leu Ala Phe Pro y 1 5 10 Ile Phe Ala y Ala Leu Ala Ala Lys Ala Lys Lys Ile Thr Pro G u 25 IVet Lys Leu Ala Ala G n Arg G n Ser G n Thr Ser G n Leu Arg Thr 40 Ser Arg Ser Ala Ala Ser Cys Leu Pro Pro Trp lie Pro Ala Leu Pro 55 Page 170 Gene Sequence 24-2-06.txt Sn G n Ser Arg G n Leu Ser Arg Pro Ser Pro Lys Arg Lys Thr Leu 70 75 Lys Asn Leu Leu Ile Asp Ala Ser Leu Pro Val u Ala Pro Ile Phe 90 <210> 127 <211> 1063 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1063) <223> FRXA00290 <400> 127 agctacagat ttagctagtg tttttgttcc agaaccctaa gagcttcccg caaaaacacc gattaacaag gctaaatgat cag G n gag G u cgc Ar g gca Al a aac Asn gat Asp ct g Leu gtt Val tct Ser gag 150 t cc Ser gga G y ctt Leu aag Lys gt c Val gga G y agc Ser gat Asp 120 ggt ggy gag G u caa G n ct c Leu ctt Leu gat Asp gtt Val cag G n ttc Phe gac Asp at c SIle cgc Ar g aac Asn t cc Ser gct Al a cca Pr o at t SIle 75 gcc Al a gct Al a gct Al a aac Asn ct c Leu 155 ct c Leu at t SIle t ac Tyr gag G u t cc Ser t cc Ser ggc G y ttg Leu ttg Leu 140 at c SIle cat Hi s t cc Ser cct Pr o gcg Al a ggc G y ccc Pr o aag Lys 110 gag G u gac Asp cgt Ar g gag G u 15 act Thr ggt G y cgc Ar g acc Thr gt c Val 95 gct Al a acc Thr at c SIle ct c Leu gaa G u cgt Ar g gtt Val acc Thr gct Al a 80 at g Met at c l Ie at c SIle t cc Ser gat Asp 160 atgaggttct acccttaaca atg acc atc gac ctg 11! IVet Thr lie Asp Leu 1 atc ttc gag gca cac 16 I e Phe G u Al a Hi s ccg ctc cgc gac atg 21 Pro Leu Arg Asp IVet gct cag gtt tgt gaa Ala n Val Cys G u cac acg ggc att gga His Thr G y lie G y gtt ctt ggc ctt ggc Val Leu y Leu y gag ggc aag gct cag G u G y Lys Al a G n 100 cct atc gtt ttg gac Pro lie Val Leu Asp 115 gca gcc atc gcg cct 49! Ala Ala lie Ala Pro 130 gct cct cgt tgc ttc 54 Ala Pro Arg Cys Phe 145 att cca gtt atg cac 59! Ile Pro Val IVet His 165 gat gac cag cac ggc acc gct gtg gtt atc ctc gct gcg ctg cgc aac Page 171 Gene Sequence 24-2-06.txt Asp Asp On His G y Thr Ala Val Val lie Leu Ala Ala Leu Arg Asn 170 175 180 tcc ctg aag ctg ctg gat cgc aag atc gaa gac ctc aag att gtt att 691 Ser Leu Lys Leu Leu Asp Arg Lys Ile G u Asp Leu Lys lie Val lie 185 190 195 tcc ggc gca ggc gca gcg ggc gtt gca gct gta gat atg ctg acc aac 739 Ser Gy Ala Gy Ala Al a y Val Ala Ala Val Asp IVet Leu Thr Asn 200 205 210 gct gga gca acc gac atc gtg gtt ctt gat tcc cga ggc atc atc cac 787 Al a y Ala Thr Asp ile Val Val Leu Asp Ser Arg G y lie lie His 215 220 225 gac agc cgt gag gat ctt tcc cca gtt aag gct gct ctt gca gag aag 835 Asp Ser Arg G u Asp Leu Ser Pro Val Lys Ala Ala Leu Ala G u Lys 230 235 240 245 acc aac cct cgt ggc atc agc ggt ggc atc aat gag gct t tc acc ggc 883 Thr Asn Pro Arg Gy lie Ser Gy Gy lie Asn u Ala Phe Thr y 250 255 260 gcg gac ctg ttc att ggc gtg tcc ggc ggc aac atc ggc gag gac gct 931 Ala Asp Leu Phe ile G y Val Ser G y y Asn ile G y Gu Asp Ala 265 270 275 ctc aaa ctc atg gcc ccg gag cca atc ctg ttc acc ctg gcg aac cca 979 Leu Lys Leu IVet Ala Pro G u Pro lie Leu Phe Thr Leu Ala Asn Pro 280 285 290 acc cca gag atc gat cct gag ctg tct cag aag tac ggc gcc atc gtc 1027 Thr Pro u lie Asp Pro u Leu Ser n Lys Tyr G y Ala lie Val 295 300 305 gcg acc ggg ccg gtc ttg acc tgc cta acc aga tca 1063 Ala Thr Gy Pro Val Leu Thr Cys Leu Thr Arg Ser 310 315 320 <210> 128 <211> 321 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 128 IVet Thr I e Asp Leu G n Arg Ser Thr G n Asn Leu Thr His G u G u 1 5 10 IIe Phe u Ala His G u y G y Lys Leu Ser lie Ser Ser Thr Arg 25 Pro Leu Arg Asp IVet Arg Asp Leu Ser Leu Ala Tyr Thr Pro y Val 40 Ala n Val Cys u Ala lie Lys u Asp Pro u Val Ala Arg Thr 55 Hi s Thr G y IIe G y Asn Thr Val Al a Val lie Ser Asp G y Thr Al a 70 75 Val Leu G y Leu G y Asp Ile G y Pro n Al a Ser Leu Pro Val bet 90 G u y Lys Ala G n Leu Phe Ser Ser Phe Ala y Leu Lys Ala lie 100 105 110 Pro lie Val Leu Asp Val His Asp Val Asp Ala Leu Val G u Thr lie Page 172 Gene Sequence 24-2-06.txt 120 Sy Ala lie Asn 125 G u Asp lie Ser Al a Al a 145 I le Al a Leu Asp Ar g 225 Al a Su I e Thr Tyr 305 l Ie Ar g Val Leu SIle 195 Leu SIle Al a Phe G u 275 Al a Pr o Phe Hi s 165 Asn SIle Asn Hi s Lys 245 Gy Al a Pr o Phe 135 Val Asp Leu Gy Gy 215 Ser Asn Asp Lys Pr o 295 u G n Lys Al a 200 Al a Ar g Pr o Leu Leu 280 G u Ar g Hi s Leu 185 Gy Thr G u Ar g Phe 265 vet SIle Ar g Gy 170 Leu Al a Asp Asp Gy 250 I le Al a Asp Leu 155 Thr Asp Al a SIle Leu 235 I le Gy Pr o Pr o Leu 140 I le Al a Ar g Gy Val 220 Ser Ser Val G u G u 300 u Val Lys Val 205 Val Pr o Gy Ser Pr o 285 Leu Ar g Val SIle 190 Al a Leu Val Gy Gy 270 I le Ser Leu SIe 175 G u Al a Asp Lys SIe 255 Gy Leu G n Sy Ala I e Val Al a 310 Thr y Pro Val Thr Cys Leu Thr <210> 129 <211> 1065 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(1042) <223> RXA02694 <400> 129 attaaaggtg t aacaaagga at ccgggcac aagctcttgc tgattttctg agctgctttg tgggttgtcc ggttagggaa atcaggaagt gggatcgaaa atg aaa gaa acc gtc 115 Net Lys G u Thr Val 1 ggt aac aag att gtc ctc att ggc gca gga gat gtt gga gtt gca tac 163 Gy Asn Lys lie Val Leu lie y Ala y Asp Val y Val Ala Tyr 15 gca tac gca ctg atc aac cag ggc atg gca gat cac ctt gcg atc atc 211 Al a Tyr Al a Leu i e Asn G n G y IVet Al a Asp Hi s Leu Al a lie i e 30 gac atc gat gaa aag aaa ctc gaa ggc aac gtc atg gac tta aac cat 259 Page 173 Asp I e Asp G u Lys Lys Leu Gene Sequence 24-2-0 G u y Asn Val let 6. txt ggt Sy gct Al a caa G n at t SIle at c SIle tgg Tr p gt c Val 150 gt g Val act Thr agc Ser at t SIle ggc Gy 230 gca Al a cac Hi s gt g Val cac Hi s gt g Val tgc Cys cca Pr o aaa Lys ct c Leu 120 ttc Phe gac Asp cca Pr o ctt Leu at g let 200 gaa G u act Thr ct a Leu gaa G u cgc Ar g 280 at g bet gcc Al a gac Asp gag G u at c SIle gcg Al a ggc Gy gct Al a t cc Ser 170 gt c Val gac Asp acc Thr t ac Tyr aac Asn 250 ggt Gy ggc Gy cgc Ar g gat t cc Asp Ser gca gcc Al a Al a 75 acc cgc Thr Ar g gtc ggc Val G y tcc aac Ser Asn ttg gaa Leu G u 140 cga ttc Arg Phe 155 gtc cac Val Hi s ctg t cc Leu Ser aaa gac Lys Asp cgc gac Arg Asp 220 ggc atc G y lie 235 caa gac Sn Asp gag gaa G u G u atc cgc li e Ar g ttc aag Phe Lys 300 cgc Ar g at g Net ct c Leu gat Asp cca Pr o 125 tgg Tr p cgc Ar g gcc Al a t cc Ser cca Pr o 205 gct Al a ggc Gy gtt Val gac Asp cgc Ar g 285 cat Hi s acc Thr gtt Val cag G n gt c Val 110 gt g Val aac Asn t ac Tyr t ac Tyr gcg Al a 190 gag G u gcc Al a at g Net gca Al a at c SIle 270 gtt Val t cc Ser cgc Ar g gt c Val ct g Leu 95 at g Net gat Asp cgc Ar g at g et at c SIle 175 acc Thr ctt Leu t at Tyr ggt Gy gt c Val 255 t ac Tyr gt c Val gca Al a gt c Val at t SIle 80 gt g Val gac Asp at c SIle gt g Val ct g Leu 160 at c SIle at c SIle gag G u cac Hi s ctt Leu 240 cca Pr o at c SIle gaa G u aat Asn acc Thr tgt Cys gac Asp agc Ser ct g Leu at c SIle 145 ggc G y ggc G y gca Al a ggc G y at t SIle 225 gct Al a gt c Val ggc Gy ct a Leu acc Thr 305 Asp aag Lys gcc Al a aaa Lys gga Gy acc Thr 130 ggc Gy gaa G u gaa G u ggc G y cgt Ar g 210 at c SIle cgc Ar g tct Ser acc Thr gaa G u 290 ct g Leu ggc G y ggc Gy aac Asn ttc Phe 115 t ac Tyr t cc Ser ct c Leu cac Hi s gt a Val 195 ct a Leu gac Asp at c SIle gca Al a cca Pr o 275 at c SIle cgc Ar g acc Thr gca Al a gt c Val 100 gac Asp gca Al a gga Gy t ac Tyr ggc G y 180 tcg Ser gag G u gcc Al a acc Thr ct g Leu 260 gct Al a acc Thr gaa G u Leu Asn His t ac Tyr gcc Al a aag Lys ggc Gy gt g Val act Thr gaa G u 165 gac Asp ctt Leu aaa Lys aag Lys cgc Ar g 245 ct c Leu gt g Val gac Asp at t SIe 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1065 cag aag cag ttc ttc taaatctttg gcgcctagtt ggc Page 174 (31n Ls 31 Pe Pe ene Sequence 24- 2- 06. txt 310 <210> 130 <211> 314 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 130 l'bt Lys (3 u Thr Val G31y Asn Lys I Ie ValI Hi s Thr cys As p Ser Le u I Ie 145 G31y G31y A a Gy le 225 A a ValI G31y Le u ValI A a Le u G31y (31y As n Ph e 115 Tyr Ser Le u Hi s ValI 1 95 Le u As p I Ie A a Pr o 275 li1e Tyr I Ie Hi s Ty r A a Ly s G31y ValI Thr (31u 1 65 As p Le u Ly s Ly s Ar g 245 Le u ValI As p A a As p G31y A a 70 (31n I Ie I Ie Tr p ValI 150 ValI Thr Ser I Ie G31y 230 A a Hi s ValI Hi s Tyr I Ie ValI As p Ly s vb t Ph e Ly s 135 Le u A a (31u Ar g Ph e 215 Ser I Ie G31y As n (31u 295 A a As p 40 ValI cys Pr o Ly s Le u 120 Ph e As p Pr o Le u vb t 200 (31u Thr Le u (31u Ar g 280 vb t Le u (31u Tr p (31u G31y Ser 1 05 ValI Ser Ser Ser Pr a 1 85 Le u As p Ser O31n Tyr 265 Ar g (31u ValI 10 I Ie Ly s A a As p G u 90 lie A a G3y A a Ser 1 70 ValI As p Thr Tyr As n 250 Gy Gy Ar g Le u As n Ly s As p A a Thr ValI Ser Le u Ar g 1 ValI Le u Ly s Ar g G31y 235 O31n O31u I Ie Ph e G31y G31y O31u Ar g vb t Le u As p Pr a 125 Tr p Ar g A a Ser Pr a 205 A a G31y ValI As p Ar g 285 Hi s A a vb t G31y Thr ValI (31n ValI 110 ValI As n Tyr Tyr A a 1 O31u A a vb t A a I Ie 270 ValI Ser G31y A a As n Ar g ValI Le u vb t As p Ar g Nibt I Ie 1 Thr Le u Tyr G31y ValI 255 Ty r ValI A a Thr Leu Arg Ou lieOn Lys On Phe Phe Page 175 ene Sequence 24- 2- 06. txt 305 310 <210O> 131 <21 1> 2967 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (2944) <223> RXN00296 <400> 131 t tt t tgt tgg cat gt ctgac at tat cgcac aat tcaccac tcaaagcccg cttggatcga atttcacaaa aggacccacc agt aaccggt aacat gt tac aaa Ly s aag Ly s gga G31y g aa O31u gt t ValI g aa O31u at t I Ie g at As p g at As p aat As n 1 50 ct c Le u ac a Th r aac As n cgc Ar g tt t Ph e at t I Ie ggg G31y t tg Le u cca Pr o t tg Le u 120 t cc Ser t gt cys g at As p g at As p ct c Le u g at As p cgc Ar g g at As p ggt G31y at c I Ie gca A a g at As p cat Hi s t ca Ser ac g Th r 1 70 t gc Cys gac As p at g vb t gt c ValI gcc A a gga G31y 75 g at As p caa O31n gca A a t cc Ser cac Hi s 155 ct c Le u g at As p cca Pr o ac a Th r cct Pr o at t I Ie gga G31y ac g Th r act Th r gcc A a cgg Ar g 140 t cg Ser at g vb t g at As p g aa (31u ac c Th r gca A a 45 gct A a ag c Ser t ca Ser gca A a gca A a 125 t gc cys gt t ValI ct c Le u gct A a gt a ValI cgt Ar g gct A a gt t ValI t cg Ser cgc Ar g gt t ValI 110 g aa (31u ac g Th r gca A a ag c Ser gag (31u 1 90 tt t Ph e 15 gca A a gt a ValI gcg A a gt t ValI t at Tyr 95 gt g ValI tt c Ph e at c I Ie tt c Ph e g at As p 1 75 at c I Ie ag c Ser gca A a gct A a gt g ValI gct A a 80 tt c Ph e g aa (31u gga G31y ggt G31y ggt G31y 1 60 ggc G31y aat As n at g vb t 1 c ag O31n t at Tyr g aa O31u gca A a 65 gga G31y aac As n ccc Pr a tt a Le u ggc G31y 145 ac a Th r cga Ar g c ag O31n cat Hi s t ct Ser cca Pr a 50 cga Ar g aat As n cgc Ar g ggt G31y act Th r 130 at g vb t gct A a g aa (31u aag Ly s act cac acc at c Thr His Thr Ilie t ct Ser t ct Ser 35 g aa (31u ggg G31y gcg A a at t I Ie gt g ValI 115 t ac Tyr gt t ValI gcg A a gt c ValI ct c Le u 1 cgc Ar g 20 g at As p aat As n t gg Tr p at c I Ie tt a Le u 1 00 gt g ValI ggc G31y gcc A a g aa (31u act Th r 1 ac c Th r gcg A a gca A a gt g ValI t ct Ser ggt G31y g at As p t gt Cys ccg Pr a aac As n aat As n 1 gt g ValI g ac As p Page 176 Gene Sequence 24-2-06.txt tta gcg tcc aag aat cag gac Leu Ala Ser Lys Asn G n Asp cct Pr o gcc Al a 230 ttg Leu ct g Leu cga Ar g gct Al a cca Pr o 310 aca Thr gca Al a gaa G u acg Thr gcg Al a 390 ct g Leu gaa G u ggc Gy gcg Al a 200 caa G n gca Al a gt g Val ttc Phe cct Pr o 280 ct g Leu aac Asn agt Ser gcc Al a cgg Ar g 360 tta Leu cct Pr o gat Asp tgc Cys aag Lys 440 t at Tyr tcg Ser gcg Al a ttg Leu 250 acg Thr gt a Val agt Ser at c SIle acc Thr 330 gac Asp ttg Leu gat Asp gag G u ttc Phe 410 cac Hi s ttc Phe ggc Gy ggc Gy ggc Gy 235 gtt Val gtt Val gca Al a aaa Lys ggc Gy 315 ct g Leu acc Thr tgg Tr p ggt Gy aat Asn 395 gat Asp gtt Val gag G u agc Ser t ac Ty r 220 acc Thr cca Pr o ttt Phe acc Thr cag G n 300 at t SIle cag G n at t SIle cgc Ar g ggg Gy 380 tta Leu t ac Tyr cgc Ar g gcg Al a ct c Leu 460 ctt att agt Leu I e Ser 205 ggt ttg cat G y Leu Hi s gag gga acc G u G y Thr aca ccc aaa Thr Pro Lys 255 gac gcc gcc Asp Al a Al a 270 att gaa ggc lie u G y 285 gga caa tca G y G n Ser gaa gcc ggc G u Al a G y gcc gcg gta Al a Al a Val 335 gat tac gt g Asp Tyr Val 350 at c cgt gaa lie Arg u 365 gaa gcg tgg G u Al a Tr p gct gat tat Al a Asp Tyr cag ggt att Gn G y lie 415 atc agt ttt Ile Ser Phe 430 ttc atg aat Phe IVet Asn 445 tcg ggc gag Ser G y G u aaa gaa Lys G u t at Tyr at t SIle 240 gt g Val cga Ar g at g bet gaa Gu gga Gy 320 caa G n gt c Val t cc Ser ccg Pr o ct c Leu 400 cca Pr o gat Asp gaa G u cat Hi s ctg ggt cgt ttc Leu y Arg Phe ctt Leu 225 gga Gy aaa Lys gca Al a ggc Gy gct Al a 305 tgg Tr p gcc Al a gt g Val tcg Ser aat Asn 385 cgc Ar g ttt Phe ttc Phe gcc Al a gga G5y 465 210 gcc Al a at c SIle gcg Al a gcc Al a gga Gy 290 ggg Gy ttg Leu gcc Al a tct Ser gcg Al a 370 tgg Tr p gat Asp gga Gy tct Ser t cc Ser 450 gac Asp gac Asp act Thr gct Al a 260 aaa Lys ct c Leu aat Asn tgc Cys gaa G u 340 cct Pr o at t l Ie gac Asp t at Tyr ttt Phe 420 aag Lys ttg Leu cgc Ar g at g Mt cgg Ar g 245 gt c Val ttg Leu ct c Leu ctt Leu gag G u 325 gt c Val tct Ser gt c Val tcg Ser gcg Al a 405 gga Gy gaa G u gt g Val gcc Al a 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 Page 177 Gene Sequence 24-2-06.txt cgc Ar g 470 ttc Phe gga Sy ccg Pr o gac Asp gca Al a 550 ggc Gy at g et aat Asn tgt Cys aaa Lys 630 tgg Tr p acg Thr cgc Ar g cgc Ar g gt g Val 710 cac Hi s t cc Ser gaa G u ttg Leu cag G n 520 ggt Gy cgc Ar g gaa Gu cgc Ar g gcc Al a 600 gt c Val t ac Tyr ccg Pr o at c SIle gt c Val 680 ct g Leu tgg Tr p gcg Al a ctt gac cgc atg Leu Asp Arg IVet 475 aag ctg att ttc Lys Leu II e Phe 490 tgg gca gat cct Trp Al a Asp Pro gcc ctc gac at c Ala Leu Asp II e 525 atg atc aac gcg IVet Ile Asn Ala 540 gaa tcc gac gcg G u Ser Asp Al a 555 cat tcc acc aga His Ser Thr Arg 570 gaa tcc atc gcc Su Ser Ile Ala gac ctg tgc ctt Asp Leu Cys Leu 605 gtc gac atg t cc Val Asp IVet Ser 620 ggc cga ctt cgc G y Arg Leu Ar g 635 ctg gga cac gtt Leu G y His Val 650 gcc acc atg cag Ala Thr Net G n ggg ctc gct gat G y Leu Al a Asp 685 aag tac aag ccg Lys Tyr Lys Pro 700 gat tcc ttc aac Asp Ser Phe Asn 715 aaa act ctt gaa Lys Thr Leu G u 730 tat tca gca gaa atg cgt gca ctc Tyr Ser Ala u IVet Arg Ala Leu gat Asp gt c Val 510 acg Thr gt g Val at g Net ggc Gy gac Asp 590 t cc Ser acc Thr ccc Pr o gcc Al a t ca Ser 670 cgc Ar g aag Lys acc Thr gcc Al a ccc Pr o 495 at g Net ccc Pr o aat Asn tgc Cys cgc Ar g 575 ggc Gy tgc Cys t ac Tyr at g et cat Hi s 655 gca Al a ccg Pr o aaa Lys aac Asn ct c Leu 735 480 cag G n caa G n gt a Val cgc Ar g ccg Pr o 560 gcc Al a t ac Tyr aaa Lys aaa Lys gcc Al a 640 aaa Lys ct c Leu ttg Leu aac Asn ct c Leu 720 ggt Gy cgc Ar g gga Gy cac Hi s tgc Cys 545 t cc Ser cgc Ar g cgc Ar g gca Al a gcc Al a 625 cat Hi s at a SIle acc Thr at t SIle t ca Ser 705 gac Asp t ac Tyr at c SIle ctt Leu aaa Lys 530 gt g Val ttc Phe ttg Leu agc Ser tgc Cys 610 gaa G u t ac Tyr ccg Pr o gcc Al a t cc Ser 690 ggt Gy acc Thr aac Asn ttc Phe cgc Ar g 515 ttc Phe ggt Gy caa G n ct c Leu gaa G u 595 gca Al a ttc Phe gt c Val ctt Leu cca Pr o 675 ttc Phe gaa G u gga Gy gt g Val aat Asn 500 at g Net t ct Ser gt a Val at c SIe t ct Ser 580 gaa G u tcg Ser ct g Leu at g bet ctt Leu 660 gt g Val gcc Al a acg Thr cca Pr o gt c Val 740 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 2275 2323 Page 178 Gene Sequence 24-2-06.txt cca gat ggc ttc gtc tgc tgt gga Pro Asp y Phe Val Cys Cys y ctc acc tgg cat tcc acc ggc caa Leu Thr Trp His Ser Thr G y O n ttg agc Leu Ser ccc tac Pro Tyr 775 acc gt c Thr Val 790 ctg gca Leu Al a cca aag Pro Lys t ca act Ser Thr cca caa Pro G n 855 caa att Gn lle 870 aaa gac Lys Asp ccc aag Pro Lys tgc cgc Cys Arg gca gag Al a G u 935 at g NMt 760 ct g Leu at g Net cgc Ar g at c SIle gcg Al a 840 caa G n gcc Al a cac Hi s gt c Val acc Thr 920 gt g Val aag Lys caa G n caa G n gca Al a 810 gag G u acc Thr gca Al a ggt Sy gaa G u 890 aaa Lys at c SIle tta Leu aaa Lys ggt G y gat Asp 795 gca Al a ct a Leu cag G n ct c Leu tgt Cys 875 at g bet gca Al a gaa G u agc Ser gt c Val ct a Leu 780 gag G u ct g Leu gt c Val gt g Val gtt Val 860 tgc Cys t cc Ser gaa G u caa G n at c SIle 940 ct a Leu 765 aca Thr gca Al a acc Thr gag G u cac Hi s 845 ctt Leu ggg Sy ttc Phe gga Sy ggc Sy 925 ttg Leu 750 gaa G u gt c Val aca Thr aaa Lys tct Ser 830 tgc Cys gaa G u ctt Leu gca Al a cat Hi s 910 acc Thr gag G u caa G n gtt Val gaa G u cca Pr o 815 gga G y cac Hi s gct Al a gcc Al a ctt Leu 895 gt g Val gga Sy caa G n acg Thr ggt G y ct c Leu 800 ttc Phe agc Ser gag Gu ttg Leu gga Sy 880 ggt Sy at t SIle aaa Lys aac Asn gcg Al a ttg Leu 785 t cc Ser gct Al a ct c Leu cgt Ar g ggt Sy 865 aac Asn gaa G u gct Al a caa G n aac Asn 945 aaa Lys 770 gaa G u gat Asp gag G u cag G n tcg Ser 850 gt a Val tgg Tr p cga Ar g gac Asp gca Al a 930 at g let gt g Val cct Pr o aac Asn gt c Val ct a Leu 835 ct a Leu aaa Lys ggc Sy gag G u ggt Sy 915 acg Thr gca Al a 755 at g NMt tcg Ser cct Pr o at c SIle 820 aca Thr ggc Gy gat Asp ttt Phe ct g Leu 900 ttc Phe cac Hi s caa G n aaa Lys tgc Cys gat Asp 805 gca Al a gaa G u gac Asp gaa G u gaa G u 885 ttc Phe t cc Ser ctt Leu 2371 2419 2467 2515 2563 2611 2659 2707 2755 2803 2851 2899 2944 2967 taacgatcat gcaacaggtg ct c <210> 132 <211> 948 <212> PRT <213> Corynebacter i um gl <400> 132 lIet Thr His Thr Ile Lys 1 5 G n His Ser Arg Ala Lys Tyr Ser Ser Asp Al a G y ut am cum Phe Asn Arg Leu Asp Pro G u Val Phe Ser 10 Leu Arg Thr Asp IVet Thr Thr Arg Ala Ala 25 Ile Phe Arg Arg Val Pro Ala Ala Val Ala Page 179 Gene Sequence 24-2-06.txt lie Arg Asp Ala Ala Val Ala Val G u Al a Gy Asn Pr o Leu Gy 145 Thr Ar g G n G u Leu 225 Gy Lys Al a Gy Al a 305 Tr p Al a Val Ser G u Gy Al a SIle Val 115 Tyr Val Al a Val Leu 195 Gy Hi s SIle Leu Al a 275 Asp G n Tyr G u G u 355 Sy Val Ser Sy Asp Cys Pr o Asn Asn 165 Val Asp Phe bet Ar g 245 Val Leu Leu Leu G u 325 Val Ser Val G u Val 70 G u SIle Asp Asp Asn 150 Leu Thr Leu Pr o Al a 230 Leu Leu Ar g Al a Pr o 310 Thr Al a G u Thr G n Val Sy Asp Al a Pr o 135 Al a Val Lys Al a Ar g 215 Lys Thr Al a Leu Al a 295 Sy Sy Thr let Ar g 375 Gy Leu Pr o Leu 120 Ser Cys Asp Asp Ser 200 G n Al a Val Phe Pr o 280 Leu Asn Ser Al a Ar g 360 Leu Ar g SIle Val 105 Ar g Thr Sy Val Sy 185 Lys Val let Lys Asp 265 G y Ar g Ar g Asp Val 345 G u Al a Gy I e 90 Al a Asp Hi s Ser Thr 170 Cys Asn Ser Al a Leu 250 Thr Val Ser SIle Thr 330 Asp Leu Asp Gy Asp G n Al a Ser Hi s 155 Leu Asp G n Sy Sy 235 Val Val Al a Lys Sy 315 Leu Thr Tr p Sy I e Gy Thr Thr Al a Ar g 140 Ser vet Asp Asp Tyr 220 Thr Pr o Phe Thr G n 300 I le G n SIle Ar g Sy 380 Ser Ser Al a Al a 125 Cys Val Leu Al a Leu 205 Sy G u Thr Asp SIle 285 Sy G u Al a Asp SIle 365 G u Ser Ar g Val 110 G u Thr Al a Ser G u 190 I le Leu Sy Pr o Al a 270 G u G n Al a Al a Tyr 350 Ar g Al a Val Tyr Val Phe SIe Phe Asp 175 I e Ser Hi s Thr Lys 255 Al a Sy Ser Sy Val 335 Val G u Tr p Asn Trp G u Asp Ser 385 Al a Val Pro Pro G u Asn Leu Ala Asp Tyr Leu Page 180 Arg Asp Phe G y Phe Ser Al a Ser 450 Sy Asp 465 Gu let Arg Ile G y Leu Hi s Lys 530 Cys Val 545 Ser Phe Arg Leu Arg Ser Al a Cys 610 Al a G u 625 Hi s Tyr Ile Pro Thr Al a I e Ser 690 Ser G y 705 Asp Thr Tyr Asn Hi s Ser Leu Tyr Ala Leu IVet 405 Gene Sequence 24-2-06.txt Asp Lys Phe Asp Tyr On Gy lie Pro 410 415 Hi s Thr 435 Thr Sy Ar g Phe Ar g 515 Phe Sy G n Leu G u 595 Al a Phe Val Leu Pr o 675 Phe G u Sy Val Thr 755 Sy G u Val Al a Leu 485 Pr o Asp Lys Ser Thr 565 G u Val G u Asp Sy 645 Pr o Val Hi s Val Al a 725 I le G n Sy Leu Ser 455 Ser G u Val Sy Lys 535 Cys Asp Phe G u Pr o 615 Hi s Leu Leu Lys Ser 695 Leu Al a Asp Ser Cys Lys 440 Tyr Ser G u Leu G n 520 a y Ar g G u Ar g Al a 600 Val Tyr Pr o SIle Val 680 Leu Tr p Al a Sy \et 760 Val 425 Lys Sy Phe Phe Val 505 Ar g Ser Ser Val Sy 585 Leu Asn Al a Leu Asp 665 Sy Ar g Pr o l Ie Phe 745 Thr Val G u Ser Asp 475 Leu Al a Leu SIle Ser 555 Ser Ser Leu Asp Ar g 635 Sy Thr Leu Tyr Ser 715 Thr Cys Ar g Al a Leu 460 Ar g SIle Asp Asp Asn 540 Asp Thr SIle Cys bet 620 Leu Hi s bet Al a Lys 700 Phe Leu Cys SIle Phe 445 Ser bet Phe Pr o SIle 525 Al a Al a Ar g Al a Leu 605 Ser Ar g Val G n Asp 685 Pr o Asn G u Sy Ser 430 vet Sy Tyr Asp Val 510 Thr Val et Sy Asp 590 Ser Thr Pr o Al a Ser 670 Ar g Lys Thr Al a Leu 750 Phe Asn G u Ser Pr o 495 vet Pr o Asn Cys Ar g 575 Sy Cys Tyr bet Hi s 655 Al a Pr o Lys Asn Leu 735 Thr Asp G u Hi s Al a 480 G n G n Val Ar g Pr o 560 Al a Tyr Lys Lys Al a 640 Lys Leu Leu Asn Leu 720 Sy Tr p Lys Lys Val Leu G u G n Thr 765 Page 181 Gene Sequence 24-2-06.txt Al a Lys 770 Leu G u 785 Ser Asp Al a G u Leu G n Arg Ser 850 G y Val 865 Asn Trp G u Ar g Al a Asp G n Al a 930 Asn IVet 945 Val Met Lys Pro Pro Ser Asn Pro Val lile 820 Leu Thr 835 Leu G y Lys Asp G y Phe G u Leu 900 G y Phe 915 Thr His Al a n Cys Asp 805 Al a G u Asp G u G u 885 Phe Ser Tyr 775 Val Al a Lys Thr G n 855 I le Asp Lys Ar g Leu Asp G n y Leu Thr Val Val y vet Ar g SIle Al a 840 G n Al a Hi s Val Thr 920 Leu Leu Thr 825 Leu Ser Thr Al a Ar g 905 G n Asp 795 Al a Leu G n Leu Cys 875 vet Al a G u Al a Thr G u Hi s 845 Leu Sy Phe Gy Sy 925 G u Pr o 815 Sy Hi s Al a Al a Leu 895 Val Sy Leu Al a G u 935 Val Val Leu Ser Leu G u G n Asn <210> 133 <211> 2858 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (1)..(2835) <223> FRXA00296 <400> 133 acc atc aaa ttc aac aga ctc gac cca Thr lie Lys Phe Asn Arg Leu Asp Pro 1 5 cgc gcg aag ctg cgc acg gat atg aca Arg Ala Lys Leu Arg Thr Asp IVet Thr 25 gat gca gga att ttt aga cgc gtc cct Asp Ala G y lie Phe Arg Arg Val Pro aat gtg gaa caa att cgt gat gcc att Asn Val u n Ile Arg Asp Ala lie 55 tgg tct gtt gtt ggg cgc ggt gga gga Trp Ser Val Val G y Arg G y G y G y 70 gaa gta ttt G u Val Phe 10 acc cgt gca Thr Arg Ala gca gct gt a Al a Al a Val gct gtt gcg Al a Val Al a agc tcg gtt Ser Ser Val 75 Page 182 cag G n t at Tyr gaa G u gca Al a gga Gy cat Hi s tct Ser cca Pr o cga Ar g aat Asn tct Ser tct Ser gaa G u ggg G y gcg Al a Gene Sequence 24-2-06.txt at c SIle tta Leu gt g Val ggc G y gcc Al a 145 gaa G u act Thr acc Thr cgt Ar g gac Asp 225 act Thr gct Al a aaa Lys ct c Leu aat Asn 305 tgc Cys gaa Gu gaa G u at t SIle gat Asp 115 gat Asp aat Asn ct c Leu aca Thr tta Leu 195 cct Pr o gcc Al a ttg Leu ct g Leu cga Ar g 275 gct Al a cca Pr o aca Thr gca Al a ttg atc atc gat Leu Ile Ile Asp cca gtt gca caa Pro Val Ala G n ttg cgc gat gca Leu Arg Asp Ala 120 tcc acg cat tcc Ser Thr His Ser 135 tgt ggt tca cac Cys G y Ser His 150 gat gtc acg ctc Asp Val Thr Leu 165 gat ggc tgc gat Asp G y Cys Asp tcc aag aat cag Ser Lys Asn G n 200 caa gtg tcg ggc G n Val Ser y 215 gca atg gcg ggc Al a Met Ala G y 230 gtg aag ttg gtt Val Lys Leu Val 245 ttc gac acg gtt Phe Asp Thr Val cct ggg gta gca Pro G y Val Ala 280 ctg cgc agt aaa Leu Arg Ser Lys 295 aac cgc atc ggc Asn Arg lie y 310 agt gac acc ctg Ser Asp Thr Leu 325 gcc gtt gac acc Al a Val Asp Thr acg tca cgc tat ttc aac cgc Thr Ser 90 act gca Thr Al a 105 gcc gca Al a Al a cgg tgc Arg Cys tcg gtt Ser Val at g ct c Iet Leu 170 gat gct Asp Al a 185 gac ctt Asp Leu tac ggt Tyr G y acc gag Thr G u cca aca Pro Thr 250 ttt gac Phe Asp 265 acc att Thr Ile cag gga G n y att gaa lie G u cag gcc G n Al a 330 att gat Ile Asp 345 Arg Tyr Phe Asn Arg gtt gtg gaa ccc ggt Val Val G u Pro y 110 gaa ttc gga tta act G u Phe G y Leu Thr 125 acg atc ggt ggc at g Thr lie G y G y IVet 140 gca ttc ggt aca gct Al a Phe G y Thr Al a 155 agc gat ggc cga gaa Ser Asp G y Arg u 175 gag atc aat cag aag G u Ile Asn G n Lys 190 att agt aaa gaa ctg lie Ser Lys G u Leu 205 ttg cat tat ctt gcc Leu His Tyr Leu Ala 220 gga acc att gga atc Sy Thr Ile Gy Ile 235 ccc aaa gtg aaa gcg Pro Lys Val Lys Ala 255 gcc gcc cga gca gcc Ala Ala Arg Ala Ala 270 gaa ggc atg ggc gga G u G y Iet G y G y 285 caa t ca gaa gct ggg G n Ser G u Al a G y 300 gcc ggc gga tgg ttg Ala Gy Gy Trp Leu 315 gcg gta caa gcc gcc Ala Val G n Ala Ala 335 tac gtg gtc gtg tct Tyr Val Val Val Ser 350 288 336 384 432 480 528 576 624 672 720 768 816 864 912 960 1008 1056 Page 183 Gene Sequence 24-2-06.txt cct Pr o at t SIle gac Asp 385 t at Tyr ttt Phe aag Lys ttg Leu cgc Ar g 465 gca Al a aat Asn at g Net tct Ser gt a Val 545 at c SIle tct Ser gaa G u tcg Ser gaa G u 355 acg Thr gcg Al a ct g Leu gaa G u ggc G y 435 gcg Al a cgc Ar g ttc Phe gga Gy ccg Pr o 515 gac Asp gca Al a ggc G y at g let aat Asn 595 tgt Cys cgg Ar g tta Leu cct Pr o gat Asp 405 tgc Cys aag Lys t at Tyr t cc Ser gaa G u 485 ttg Leu cag G n ggt Sy cgc Ar g gaa G u 565 cgc Ar g gcc Al a gt c Val gaa G u gct Al a cca Pr o 390 aag Lys gt c Val aaa Lys ggt Gy tt c Phe 470 tt c Phe gt c Val cgc Ar g t cc Ser t ca Ser 550 gt a Val ggt G y ctt Leu aac Asn ttg tgg Leu Trp 360 gat ggt Asp G y 375 gag aat Su Asn ttc gat Phe Asp cac gtt His Val ttc gag Phe 0 u 440 ggc agc G y Ser 455 ctt gac Leu Asp aag ct g Lys Leu tgg gca Trp Al a gcc ct c Al a Leu 520 at g at c let lie 535 gaa t cc Su Ser cat t cc Hi s Ser gaa t cc Su Ser gac ctg Asp Leu 600 gtc gac Val Asp 615 cgc at c cgt Arg II e Ar g gaa G u ggg G y tta Leu t ac Tyr cgc Ar g 425 gcg Al a ct c Leu cgc Ar g at t SIle gat Asp 505 gac Asp aac Asn gac Asp acc Thr at c SIle 585 tgc Cys at g Net gaa G u gct Al a cag G n 410 at c SIle ttc Phe tcg Ser at g tet ttc Phe 490 cct Pr o at c SIle gcg Al a gcg Al a aga Ar g 570 gcc Al a ct t Leu gcg Al a gat Asp 395 ggt G y agt Ser at g Net ggc Gy t at Tyr 475 gat Asp gt c Val acg Thr gt g Val at g Net 555 ggc G y gac Asp t cc Ser t cc Ser 365 ccg Pr o ct c Leu cca Pr o gat Asp gaa G u 445 cat Hi s gca Al a cag G n caa G n gt a Val 525 cgc Ar g ccg Pr o gcc Al a t ac Tyr aaa Lys 605 tcg gcg ggc Ser Al a G y aat Asn cgc Ar g ttt Phe ttc Phe 430 gcc Al a gga Gy gaa G u cgc Ar g gga Gy 510 cac Hi s tgc Cys t cc Ser cgc Ar g cgc Ar g 590 gca Al a tgg Tr p gat Asp gga G y 415 tct Ser t cc Ser gac Asp at g Net at c SIle 495 ctt Leu aaa Lys gt g Val ttc Phe ttg Leu 575 agc Ser tgc Cys gaa G u ctt Leu 400 cac Hi s acc Thr acc Thr ggt Gy cgt Ar g 480 ttc Phe cgc Ar g ttc Phe ggt G y caa G n 560 ct c Leu gaa G u gca Al a 1104 1152 1200 1248 1296 1344 1392 1440 1488 1536 1584 1632 1680 1728 1776 1824 1872 t cc acc t ac Ser Thr Tyr 620 Page 184 aaa gcc gaa ttc Lys Al a G u Phe Gene Sequence 24-2-06.txt ct g Leu 625 at g Met ctt Leu gt g Val gcc Al a acg Thr 705 cca Pr o gt c Val ggc Gy at g bet tcg Ser 785 cct Pr o at c SIle aca Thr ggc G y gat Asp 865 aaa Lys tgg Tr p acg Thr cgc Ar g 675 cgc Ar g gt g Val cac Hi s cca Pr o ttg Leu 755 ccc Pr o acc Thr ct g Leu cca Pr o t ca Ser 835 cca Pr o caa G n t ac Tyr ccg Pr o 645 at c SIle gt c Val ct g Leu tgg Tr p gcg Al a 725 ggc Gy at g bet ct g Leu at g Met cgc Ar g 805 at c SIle gcg Al a caa G n gcc Al a gcc Al a 630 ctt Leu gac Asp ggc G y cgc Ar g ccc Pr o 710 at c l Ie tt c Phe aca Thr gac Asp ctt Leu 790 ctt Leu acc Thr ctt Leu tcg Ser act Thr 870 ggc Gy ct g Leu gcc Al a ggg G y aag Lys 695 gat Asp aaa Lys gt c Val aag Lys caa G n 775 caa G n gca Al a gag G u acc Thr gca Al a 855 ggt G y cga ctt cgc ccc Arg Leu Arg gga cac gt t G y Hi s Val 650 acc atg cag Thr Met G n 665 ct c gct gat Leu Al a Asp 680 tac aag ccg Tyr Lys Pro tcc ttc aac Ser Phe Asn act ctt gaa Thr Leu G u 730 tgc tgt gga Cys Cys G y 745 aaa gt c ct a Lys Val Leu 760 ggt ct a aca G y Leu Thr gat gag gca Asp G u Al a gca ct g acc Ala Leu Thr 810 ct a gt c gag Leu Val G u 825 cag gtg cac G n Val Hi s 840 ctc gtt ctt Leu Val Leu tgt tgc ggg Cys Cys G y atg tcc ttc Iet Ser Phe 890 Pr o 635 gcc Al a t ca Ser cgc Ar g aag Lys acc Thr 715 gcc Al a ct c Leu gaa G u gt c Val aca Thr 795 aaa Lys tct Ser tgc Cys gaa G u ctt Leu 875 gca Al a atg gcc [et Al a cat aaa Hi s Lys gca ct c Al a Leu ccg ttg Pro Leu 685 aaa aac Lys Asn 700 aac ct c Asn Leu ctc ggt Leu G y acc tgg Thr Trp caa acg G n Thr 765 gtt ggt Val G y 780 gaa ct c G u Leu cca ttc Pro Phe gga agc G y Ser cac gag Hi s G u 845 gct ttg Al a Leu 860 gcc gga Al a G y ctt ggt Leu G y cat tac gt c Hi s Tyr Val 640 at a ccg ct t Ile Pro Leu 655 acc gcc cca Thr Ala Pro 670 att tcc ttc Ile Ser Phe t ca ggt gaa Ser G y G u gac acc gga Asp Thr G y 720 t ac aac gt g Tyr Asn Val 735 cat t cc acc Hi s Ser Thr 750 gcg aaa gt g Al a Lys Val ttg gaa cct Leu G u Pro t cc gat aac Ser Asp Asn 800 gct gag gt c Al a u Val 815 ct c cag ct a Leu G n Leu 830 cgt tcg ct a Arg Ser Leu ggt gt a aaa G y Val Lys aac tgg ggc Asn Trp y 880 1920 1968 2016 2064 2112 2160 2208 2256 2304 2352 2400 2448 2496 2544 2592 2640 2688 ttt gaa aaa gac cac gct gaa Phe G u Lys Asp His Ala u 885 gaa G u cga gag Arg G u 895 Page 185 Gene Sequence 24- 2- 06. txt ctg ttc ccc aag gtc aga aaa gca gaa gga cat gtg att gct gac ggt Leu Phe Pro Lys Val Arg Lys Ala G u G y His Val IIe Ala Asp G y 900 905 910 ttc tcc tgc cgc acc cag atc gaa caa ggc acc gga aaa caa gca acg Phe Ser Cys Arg Thr n Ile G u Gn y Thr y Lys n Ala Thr 915 920 925 cac ctt gca gag gtg gtc tta agc atc ttg gag caa aac aac atg gca His Leu Ala G u Val Val Leu Ser Ile Leu G u n Asn Asn et Al a 930 935 940 caa taacgatcat gcaacaggtg ctc G n 945 <210> 134 <211> 945 <212> PRT <213> Cor ynebact eri um gl ut ari cum 2736 2784 2832 2858 Thr 1 Ar g Asp Asn Tr p I le Leu Val Sy Al a 145 G u Thr Thr Ar g Asp 225 0> 134 lie Lys Phe Asn Arg Leu Asp Pro Ala Lys Leu Al a G y l e Val u n Ser Val Val Sy u y Asp Ile Asp 100 Cys Asp Al a 115 Pro Asp Pro 130 Asn Asn Al a Asn Leu Val Val Thr Lys 180 Asp Leu Al a 195 Phe Pro Ar g 210 Iet Al a Lvs 5 Ar g Phe SIle Sy Leu Pr o Leu Ser Cys Asp 165 Asp Ser G n Al a Asp Ar g Asp 55 Sy SIle Al a Asp Hi s 135 Ser Thr Cys Asn Ser 215 Al a Thr Pr o SIle Sy Thr Thr 105 Al a Ar g Ser et Asp 185 Asp Tyr Thr G u 10 Thr Al a Al a Ser Ser 90 Al a Al a Cys Val Leu 170 Al a Leu Sy Val Ar g Al a Val Ser 75 Ar g Val G u Thr Al a 155 Ser G u SIle Leu Phe Al a Val Al a Val Tyr Val Phe SIle 140 Phe Asp SIle Ser Hi s 220 Ser Al a Al a Val Al a Phe G u Sy 125 Sy Sy Sy Asn Lys 205 Tyr Hi s Ser Pr o Ar g Asn Ar g Sy Thr bet Al a G u 175 Lys Leu Al a Ser Ser G u Sy Al a I e Val Tyr Val Al a 160 Val Leu Sy Hi s Su G y 235 Thr I e G y Ile Ie 240 Page 186 Gene Sequence 24-2-06.txt Thr Al a Lys Leu Asn 305 Cys G u Pr o SIle Asp 385 Tyr Phe Lys Leu Ar g 465 Al a Asn let Ser Val 545 I le Ser Arg Leu Thr Val Leu Al a 260 Leu Arg Leu 275 Leu Al a Al a 290 Leu Pro G y G u Thr G y Val Al a Thr 340 Ser G u lbet 355 Val Thr Ar g 370 Ser Al a Val Al a Leu IVet G y G u G y 420 G u G y Leu 435 Val Al a Ser 450 Al a Arg Ser Leu Phe G u Pro G y Val 500 Asp Pro y 515 Lys Asp Lys 530 Ser Al a Cys Thr G y Asp G u let Phe 580 Val Lys Leu Val Pro Thr Pro Lys Val Lys Ala Leu 245 Phe Pr o Leu Asn Ser 325 Al a Ar g Leu Pr o Asp 405 Cys Lys Tyr Ser G u 485 Leu G n Sy Ar g G u 565 Ar g Asp a y Ar g Ar g 310 Asp Val G u Al a Pr o 390 Lys Val Lys Sy Phe 470 Phe Val Ar g Ser Ser 550 Val Sy Thr Val Ser 295 I le Thr Asp Leu Asp 375 G u Phe Hi s Phe Sy 455 Leu Lys Tr p Al a et 535 G u Hi s G u Phe 265 Thr G n SIle G n SIle 345 Ar g Gy Leu Tyr Ar g 425 Al a Leu Ar g SIle Asp 505 Asp Asn Asp Thr SIle 585 250 Asp SIle Sy G u Al a 330 Asp SIle G u Al a G n 410 I le Phe Ser let Phe 490 Pr o SIle Al a Al a Ar g 570 Al a Al a G u G n Al a 315 Al a Tyr Ar g Al a Asp 395 Sy Ser bet Sy Tyr 475 Asp Val Thr Val Net 555 Sy Asp Al a Sy Ser 300 Sy Val Val G u Tr p 380 Tyr SIle Phe Asn G u 460 Ser Pr o let Pr o Asn 540 Cys Ar g Sy Ar g let 285 G u Sy G n Val Ser 365 Pr o Leu Pr o Asp G u 445 Hi s Al a G n G n Val 525 Ar g Pr o Al a Tyr Al a 270 Sy Al a Tr p Al a Val 350 Ser Asn Ar g Phe Phe 430 Al a Sy G u Ar g Sy 510 Hi s Cys Ser Ar g Ar g 590 255 Al a Al a Sy Asp G y G n Leu Tyr 320 Al a u 335 Ser G u Al a y Trp u Asp Leu 400 G y Hi s 415 Ser Thr Ser Thr Asp y IVet Arg 480 lie Phe 495 Leu Arg Lys Phe Val G y Phe G n 560 Leu Leu 575 Ser G u G u Val Asn G u Ala Leu Asp Leu Cys Leu Ser Cys Lys Ala Cys Ala Page 187 Gene Sequence 24-2-06.txt 595 600 605 Ser G u Cys Pro Val Asn Val Asp Met Ser Thr Tyr Lys Ala G u Phe 610 615 620 Leu Asp Lys His Tyr Al a G y Arg Leu Arg Pro et Al a His Tyr Val 625 630 635 640 IVet G y Trp Leu Pro Leu Leu G y Hi s Val Ala His Lys IIe Pro Leu 645 650 655 Leu Pro Thr Leu Ile Asp Ala Thr IVet G n Ser Ala Leu Thr Ala Pro 660 665 670 Val Val Arg Lys Val G y y Leu Ala Asp Arg Pro Leu lie Ser Phe 675 680 685 Ala His Arg Ser Leu Arg Lys Tyr Lys Pro Lys Lys Asn Ser G y G u 690 695 700 Thr Val Val Leu Trp Pro Asp Ser Phe Asn Thr Asn Leu Asp Thr y 705 710 715 720 Pro Ala His Ala Ala I e Lys Thr Leu G u Ala Leu G y Tyr Asn Val 725 730 735 Val lie Pro Asp G y Phe Val Cys Cys G y Leu Thr Trp His Ser Thr 740 745 750 G y G n Leu Ser IVet Thr Lys Lys Val Leu G u G n Thr Ala Lys Val 755 760 765 IVet Lys Pro Tyr Leu Asp n G y Leu Thr Val Val G y Leu G u Pro 770 775 780 Ser Cys Thr Val Iet Leu G n Asp G u Ala Thr G u Leu Ser Asp Asn 785 790 795 800 Pro Asp Leu Ala Arg Leu Ala Ala Leu Thr Lys Pro Phe Ala G u Val 805 810 815 lie Ala Pro Lys Ile Thr G u Leu Val u Ser y Ser Leu G n Leu 820 825 830 Thr u Ser Thr Ala Leu Thr G n Val His Cys His G u Arg Ser Leu 835 840 845 Sy Asp Pro n G n Ser Ala Leu Val Leu G u Ala Leu y Val Lys 850 855 860 Asp G u n lie Al a Thr G y Cys Cys G y Leu Al a G y Asn Trp G y 865 870 875 880 Phe G u Lys Asp Hi s Al a G u IVet Ser Phe Al a Leu G y G u Arg G u 885 890 895 Leu Phe Pro Lys Val Arg Lys Ala G u G y His Val IIe Ala Asp G y 900 905 910 Phe Ser Cys Arg Thr n Ile u n y Thr y Lys n Ala Thr 915 920 925 His Leu Ala G u Val Val Leu Ser Ile Leu G u n Asn Asn Iet Ala 930 935 940 G n 945 Page 188 ene Sequence 24- 2- 06. t xt <210> <211> <212> <213> <220> <221> <222> <223> 135 1383 DNA Cor ynebact er i ur gi ut arri cum CDS (1360) R(AO 19 01 <400> 135 gcatgttgcc ttctctctgt gatcgcctcg ttcttcatcc aactaaaatc taagtaaaac ccctccgaaa ggaaccaccc aacgcgtcgc gcaccaagag atg gtg aaa cgt caa 11! I'bt Val Lys Arg n 1 ctg ccc aac ccc Leu Pro Asn Pro gca Al a gaa cta ctc gaa 3 u Leu Leu 3 u atg aag ttc aaa I'bt Lys Phe Lys aag cca Lys Pro gag ctc aac 3 u Leu Asn gac ctg cgt Asp Leu Arg aag aaa cga cgc Lys Lys Arg Arg ct a Leu gac tcc gcg ctc Asp Ser Ala Leu acc at c t ac Thr lIe Tyr gcg ttc gac Al a Phe Asp aaa att gct aaa Lys IHe Ala Lys cga Ar g cgc acc cca gct Arg Thr Pro Ala tac acc Tyr Thr gac ggc gca gcc Asp 0y Al a Al a gcc gaa ctc tca Al a 3 u Leu Ser aca cgc gca cgt Thr Arg Ala Arg gaa 1u gca ttc gaa aac Al a Phe 3 u Asn gaa ttc cac cca Ou Phe His Pro gac Asp 80 at c ct c aag cct Ile Leu Lys Pro gca Al a gaa cac gta gac 0u His Val Asp acc Thr acc acc caa atc Thr Thr 0 n II e ggc gga acc tcc 0 y 0 y Thr Ser t cc at g Ser I'bt 100 cca ttc ggc Pro Phe 3y gca cca acc ggc Ala Pro Thr Gy acc cgc ctc atg Thr Arg Leu I'bt cag acc gaa 0 n Thr 0 u 115 ggt gaa atc gca ggt gcc gga gct gca ggc gct gca gga att cct ttc 0y 0u Ile Al a 0y Al a 0y Al a Al a 0y Al a Al a 0y Ile Pro Phe 120 125 130 acc ctg Thr Leu 135 tcc acc ctg ggc Ser Thr Leu 0 y act Thr 140 acc tcc atc gaa Thr Ser lIe u gtc aag gcc acc Val Lys Al a Thr aac Asn 150 ccc aac ggc cga Pro Asn 3y Arg tgg ttc cag ctc Trp Phe On Leu t ac Tyr 160 gtc atg cgc gac Val I'bt Arg Asp gaa at c t cc t ac 0u IHe Ser Tyr ggc 31y 1 70 ctc gtc gaa cgc Leu Val 3u Arg gca Al a 175 gcc aaa gca gga Al a Lys Al a 0y ttc gac Phe Asp 180 acc ct g at g Thr Leu I'bt acc gt g gat acc Thr Val Asp Thr ccc Pr o 190 atc gcc ggc tac II e Al a 0 y Tyr cgc atc cgc Ar g I I e Ar g 195 gat tcc cgc aac gga ttc tcc atc ccg Asp Ser Arg Asn Gy Phe Ser Ile Pro cca cag ctg acc cca tcc acc Pro On Leu Thr Pro Ser Thr Page 189 ene Sequence 24- 2- 06. txt 200 gt g ValI ac c Th r 230 ggc G31y ct c Le u gt c ValI ggc G31y gt c ValI 310 cca Pr o gca A a ct c Le u gcc A a t cc Ser 390 at g b t aat gca As n A a acc ctt Thr Leu ct c ct c Leu Leu gt c at c Val I Ie 265 aac gtt As n Val 280 at c ct c I I e Leu ttc cac Phe Hi s at c at g I e I'b t gt a gcc Val Ala 345 gga ct C G3 y Leu 360 ct C cgc Leu Arg ct C gaa Leu G3 u cca gtt Pr o Val atc cca I Ie Pr o gag tt c G3 u Phe 235 aac t cc Asn Ser 250 cgt gaa Ar g G31u gaa gac 31u As p t cc aac Ser As n ct c ct g Leu Leu 315 at c gac I I e As p 330 atg ggc Vb t (1y atg gcc [vb t A a agc gag Ser G3 u gaa ct C a3 u Leu 395 tct gac Ser As p 410 cgc Ar g 220 gca A a gcg A a at g vb t t cc Ser c ac Hi s 300 cca Pr o ac c Thr gct A a gga a31y at c I Ie 380 gag a31u gca A a 205 cca Pr o t cc Ser at g vb t t gg Tr p gt c ValI 285 ggt a31y c ag a31n a31y g ac As p ggc a31y 365 ac c Thr cca Pr o t gg Tr p ct t Le u g at As p cca Pr a 270 aaa Ly s ggc a31y gt a ValI at c I Ie tt c Ph e 350 cgc Ar g cgc Ar g cgc Ar g t gg Tr p t cg Ser 240 ac c Thr aag Ly s ct c Le u c aa a31n aag Ly s 320 aac As n ct c Le u ggc a31y at g vb t gt c ValI 400 210 g ac As p ggc a31y t ct Ser gt a ValI c aa a31n 290 g ac As p gt c ValI gcc A a ggt a31y g ac As p 370 ct c Le u c ag a31n tt c Ph e gga a31y t ac Tyr gt c ValI 275 ggc a31y cgc Ar g gga a31y g ac As p cgt Ar g 355 cgc Ar g ct c Le u ct g Le u ct g Le u ac c Thr g aa a31u 260 aag Ly s gt c ValI gca A a t ct Ser at c I Ie 340 gcc A a ac c Thr ggt a31y gcc A a 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 1315 1360 1383 act cgt t ct gca gcg gcg gag at t Thr Arg Ser Ala Ala Ala au Ilie 415 420 taaaagtttc tctccttagc tat <210O> 136 <21 1> 420 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 136 I'bt Val Lys Arg a31n Leu Pro Asn Pro Ala a31u Leu Leu a31u Leu [Vbt 1 5 10 Lys Phe Lys Lys Pr o (3u Leu Asn (3y Lys Lys Arg Arg Leu Asp Ser 25 Page 190 ene Sequence 24- 2- 06. txt Ala Leu Thr I Ie Tyr Asp Leu Ar Lys I I e A a Lys %rg Arg Thr Pro A a lIe lie G31y Le u A a As p 145 ValI Ly s G31y Le u I Ie 225 Thr I Ie Le u As p Le u 305 O31u G31y I Ie ValI A a Ar g Ly s Ser O31n 115 li1e Ly s Ar g G31y Ar g 1 95 Pr o Ph e G31y Tyr ValI 275 G31y Ar g G31y As p Ar g 355 Ar g As p Ar g A a vb t O31u Ph e Thr Ar g 1 65 As p Ar g Thr Thr ValI 245 As p G31y As p Pr o O31u 325 ValI Tyr I Ie Tyr O31u 70 O31u Pr o G31y Thr As n 150 (31u Thr As p ValI Thr 230 G31y Le u ValI G31y ValI 310 Pr a A a Le u A a Thr A a Hi s Ph e O31u Le u 135 Pr a I Ie Le u Ser Le u 215 Pr a As p Ly s O31n Le u 295 Pr a Thr A a Tyr I Ie 375 O31y O31u As p I Ie 1 A a Thr O31y Tyr Ph e 1 As n A a Le u Le u I Ie 265 ValI Le u Hi s vb t A a 345 Le u Ar g A a As n Thr 90 A a O31y Le u Ar g (31y 1 70 Thr Oy le Ou As n 250 Ar g O31u Ser Le u I Ie 330 vb t vb t Ser A a lIe 75 Thr Pr a A a O31y As n 1 55 Le u ValI Ph e Pr a Ph e 235 Ser O31u As p As n Le u 315 As p O31y A a O31u O31u O31u Thr Thr O31y Thr 140 Tr p ValI As p Ser Ar g 220 A a A a vb t Ser Hi s 300 Pr a Thr A a G31y 380 A a Ph e O31n G31y A a 125 Thr Ph e (31u Thr I Ie 205 Pr a Ser vb t Tr p ValI 285 G31y O31n O31y As p O31y 365 Thr Le u Pr a Le u Thr O31y Le u A a 1 li1e Pr a Tr p Ser Pr a 255 O31y Le u Ar g Ar g vb t 335 Thr O31u Thr Ser As p O31y Ar g A a O31u Tyr 1 A a A a O31n Tr p Ser 240 Thr Ly s Le u O31n Ly s 320 As n Le u O31y vb t Ala Leu Leu O31y Val Ser Ser Leu O31u O31u Leu O31u Pro Ar g Hi s Val Page 191 385 Thr G Al a Al Gene Sequence 24-2-06.txt 390 395 400 n Leu Ala Lys IVet Val Pro Val Ser Asp Ala Thr Arg Ser Ala 405 410 415 a Gu lie 420 <210> 137 <211> 1836 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1813) <223> RXN01952 <400> 137 ccatcaaaaa atgaacgacc gcggactagc tcggatcaag tgacgcgctt gtgatgcaac tgaatatagg aagcttagag gcgacatccc ctcagcatca atg acg Met Thr I cag G n gt c Val agc Ser ccc Pr o aac Asn gga Gy tcg Ser gcg Al a gcc Al a ggc Gy 150 acc Thr gac Asp ggc Gy acg Thr ct c Leu ggc Gy cac Hi s tcg Ser 120 cac Hi s tcg Ser act Thr cat Hi s cga Ar g gt c Val gt c Val ggc Gy at c SIle gcg Al a cgc Ar g at c SIle tcg Ser gt a Val tt c Phe gag G u at c SIle 75 tt c Phe gat Asp ggc Gy gag G u ggc Gy 155 cac Hi s ct g Leu ggc Gy at g bet ccg Pr o caa G n gag G u acc Thr ccg Pr o 140 ggc Gy gcg Al a tct Ser gga Gy cgg Ar g gca Al a t ac Tyr cac Hi s 110 ct g Leu tcg Ser gcg Al a at c I e 15 gag G u cca Pr o gcg Al a tcg Ser gat Asp 95 ct c Leu aca Thr gt g Val aac Asn gat Asp cgt Ar g gt c Val ct g Leu aac Asn 80 cgc Ar g at c SIle cac Hi s at c SIle aac Asn 160 1 gcg Al a gcc Al a ttc Phe cag G n acg Thr ccc Pr o aac Asn ct g Leu ggg Gy 145 tcg Ser ttc Phe acg Thr gcc Al a gt a Val ggc Gy at t SIle gac Asp acc Thr 130 tcg Ser ggc Gy caa cca gga G n Pro G y aag aga atc Lys Arg Ile atg cca ttc IVet Pro Phe gtg gtg cgc Val Val Arg t cc gtc gac Ser Val Asp ctg act ggt Leu Thr G y gt g at c at c Val Ile lle 100 gcg cgc gag Al a Ar g G u 115 gac gcg ctc Asp Al a Leu aca t ca at c Thr Ser Ile ggc agc cag G y Ser G n 165 att cgc aag ggt ccg gca lie Arg Lys y Pro Al a ttc acg cgc gaa gcg Phe Thr Arg u Ala atc ttc gcc cgc gtc lle Phe Ala Arg Val Page 192 Gene Sequence 24-2-06.txt aac Asn gga Gy tct Ser t ac Tyr 230 gcg Al a at g Met acc Thr cgt Ar g t ac Tyr 310 acc Thr ttc Phe at t SIle ct g Leu gag G u 390 gag G u ttc Phe gac Asp gac Asp 200 gag G u gag G u ccc Pr o ttc Phe ttt Phe 280 ttg Leu ggc Gy gt c Val ttc Phe ccg Pr o 360 aac Asn cac Hi s at g Met at c SIle aag Lys gag G u gt c Val ttg Leu t ac Tyr 250 gt g Val at c SIle ct c Leu agt Ser ct g Leu 330 acg Thr ttc Phe ct g Leu ct g Leu aag Lys 410 acg Thr gt c Val gt c Val acc Thr cgc Ar g 235 ct g Leu cgc Ar g ggc Gy gaa G u gcc Al a 315 aag Lys tgg Tr p gcc Al a ccc Pr o ct c Leu 395 gag G u tct Ser gag G u gca Al a cca Pr o 220 aag Lys ttc Phe acc Thr acg Thr gcc Al a 300 ttc Phe ttc Phe gcc Al a gac Asp aag Lys 380 acc Thr ttc Phe gat Asp ct g Leu ct c Leu 205 gct Al a at c SIle gag G u cgc Ar g aac Asn 285 gac Asp gac Asp at g Net aac Asn acg Thr 365 cgc Ar g gt c Val ttc Phe gaa G u gt c Val 190 gac Asp ccc Pr o gt g Val gct Al a acc Thr 270 aac Asn at g tet ttg Leu agt Ser ggc Gy 350 gt a Val at g et agc Ser gca Al a gaa G u 430 175 aat Asn cgt Ar g gaa G u cct Pr o t cc Ser 255 ttc Phe acg Thr ccg Pr o gcc Al a cca Pr o 335 ttg Leu tcg Ser at g Net gag G u gag G u 415 aag Lys cac Hi s ct a Leu gac Asp tcg Ser 240 ggc Gy cct Pr o cac Hi s ct g Leu gag G u 320 gcg Al a ttc Phe caa G n gag G u tcg Ser 400 ccc Pr o agc Ser ct g Leu cag G n tcg Ser 225 cct Pr o tcg Ser cgc Ar g gag G u cct Pr o 305 aag Lys ct g Leu tcg Ser gcc Al a t ac Tyr 385 cag G n gag G u gcg Al a ggc Gy cgc Ar g 210 aac Asn gct Al a gcc Al a gaa G u ct c Leu 290 at c SIle t ac Tyr cag G n aag Lys at g Net 370 cgc Ar g aag Lys cac Hi s tcg Ser tcg Ser gag G u acc Thr t ac Tyr aag Lys 260 cac Hi s gag G u ggt Gy aaa Lys cgc Ar g 340 ccc Pr o agc Ser cgt Ar g gcg Al a ggt Gy 420 aac Asn 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 ttc ggc gcg gcc agt gcc gcc act cgc tac gcc gcg ttg aag cgc cgg Phe y Ala Ala Ser Al a Ala Thr Arg Tyr Ala Ala Leu Lys Arg Arg Page 193 cac Hi s tgg Tr p 470 gt c Val t at Tyr cag G n t ac Tyr gag G u 550 ccg Pr o 440 gca Al a tgg Tr p gcg Al a gcc Al a ct g Leu 520 cgc Ar g gat Asp aag Lys ct c Leu gag G u t ac Tyr 490 cag G n gag G u t ac Tyr acg Thr tgg Tr p 570 Gene Sequence 24-2-06.txt 445 450 ccc atc gat gtg gcc ctg cgt cgc Pro lie Asp Val Ala Leu Arg Arg 460 465 ctg ccg gag gag atc gac gac cag Leu Pro u u lie Asp Asp n 480 cac ttc ttc tgc cat gtg atg cac His Phe Phe Cys His Val Met His 495 gtg gat ctc gag gcg ctg cac gac Val Asp Leu G u Ala Leu His Asp 510 515 cgc ggc gcg aag ctg ccc gcc gag Arg y Ala Lys Leu Pro Ala G u 525 530 ctg ccg gag tcc atg gaa gag cac Leu Pro u Ser IVet u u His 540 545 acg ttc aac gcc ggt atc ggc ggc Thr Phe Asn Ala Gy lie Gy y 560 taagtcccca aggtagcgcg acg gac Asp ctt Leu cag G n 500 cgc Ar g cac Hi s ttc Phe acg Thr 1507 1555 1603 1651 1699 1747 1795 1836 <210> 138 <211> 571 <212> PRT <213> Corynebact <400> 138 et Thr G n Pro 1 Ala Phe Lys Arg Ala Thr Net Pro Phe Al a Val Val G n Val Ser Val Thr G y Leu Thr Pro I I e Val li e 100 Asn Asp Ala Arg 115 Leu Thr Asp Ala 130 er i um gl ut ari cum G y G n Thr Thr Thr 5 I e Val Gy Asp u 25 Phe Ser Lys G y Tyr 40 Arg Pro y Thr Leu Asp Asn Asn Leu Ile 70 Gy y Ser y Pro Ile Ser Thr His Arg 105 G u Al a lie Ser Leu 120 Leu Al a Lys His G n 135 Thr Ser I 10 His Val I Arg Phe I Val G u Val Ile I G y Phe 90 Ile Asp Al a G y Arg u I Page 194 G u Thr Gy Tr p G n Asp Val Pr o 125 Hi s Al a Ser Sy Ar g Al a Tyr Hi s 110 Leu Ser I e G u Pr o Al a Ser Asp Leu Thr Val Gene Sequence 24-2- 06. txt G31y 145 Ser I Ie Le u O31n Ser 225 Pr o Ser Ar g O31u Pr o 305 Ly s Le u Ser A a Tyr 385 O31n O31u A a A a Le u 465 As p ValI Ser Thr Ser I Ie Ala Ser Val Ilie O31y A a I Ie 1 95 O31y O31u Ar g O31y ValI 275 O31u Ser O31y Thr I Ie 355 Ph e As n A a Thr Le u 435 Ly s Ar g O31n Hi s O31n 1 65 ValI Le u Tr p O31u As n 245 Le u Pr o I Ie O31u As p 325 vb t O31y ValI Ph e Ser 405 O31u Ar g Ar g As p O31u 485 As p Ar g As p As p Pr a 215 A a As n ValI ValI Ar g 295 vb t Ph e Ser O31y Pr a 375 Hi s Ly s Ph e O31y I Ie 455 As n Ly s ValI Ly s As p As p 200 O31u O31u Pr a Ph e Ph e 280 Le u O31y ValI Ph e Pr a 360 As n Hi s vb t I Ie A a 440 A a Tr p A a A a O31y O31y 1 Pr a As p Hi s O31u A a 265 Tyr Ph e Ar g Ph e Ly s 345 Thr O31n Le u Le u cys 425 A a O31y Le u Tyr Ly s 505 (31y 1 55 A a ValI ValI Thr Ar g 235 Le u Ar g O31y O31u A a 315 Ly s Tr p A a Pr a Le u 395 O31u Ser A a I Ie ValI 475 O31y Ph e O31u A a Pr a 220 Ly s Ph e Thr Thr A a 300 Ph e Ph e A a As p Ly s 380 Thr Ph e As p A a Pr a 460 Le u Hi s G y Il e Al aAsn Asn Thr Le u Le u 205 A a I Ie O31u Ar g As n 285 As p As p vb t As n Thr 365 Ar g ValI Ph e O31u Thr 445 li1e Pr a Ph e O31u 1 As n Ar g O31u Pr a Ser 255 Ph e Thr Pr a A a Pr a 335 Le u Ser vb t O31u O31u 415 Ly s Tyr ValI O31u cys 495 1 A a Hi s Le u As p Ser 240 O31y Pr a Hi s Le u O31u 320 A a Ph e O31n O31u Ser 400 Pr a Ser A a A a I Ie 480 Hi s On Oy Val Asp Leu Ou Ala 510 Page 195 Gene Sequence 24-2-06.txt Leu His Asp Arg lie G n His Leu Leu G u G u Arg y Ala Lys Leu 515 520 525 Pro Ala G u His Asn Tyr G y Arg IVet Tyr Lys Leu Pro G u Ser IVet 530 535 540 G u G u His Phe Lys G u Leu Asp Pro Thr Asn Thr Phe Asn Ala G y 545 550 555 560 IIe G y G y Thr Ser Pro His Lys Asp Trp Ala 565 570 <210> 139 <211> 239 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> <223> FRXA01952 <400> 139 cca gga cta tgt cgc caa gca ggg cgt gga tct caa ggc gct gac gac 48 Pro G y Leu Cys Arg n Al a y Arg G y Ser n G y Al a Asp Asp 1 5 10 cgc atc cag cac ttg ctg gag gag cac ggc aag aag ctg ccc gcc gag 96 Arg lie G n His Leu Leu u u His Gy Lys Lys Leu Pro Ala G u 25 cac aac tac ggt cgc atg tac aag ctg ccg gag tcc atg gaa gag cac 144 Hi s Asn Tyr G y Arg IVet Tyr Lys Leu Pro G u Ser Iet G u G u Hi s 40 ttc aag gag ctc gat ccg acg aat acg ttc aac gcc ggt atc ggc ggc 192 Phe Lys G u Leu Asp Pro Thr Asn Thr Phe Asn Ala Gy lie Gy y 55 acg tcg ccg cac aag gac tgg gcc taagtcccca aggtagcgcg acg 239 Thr Ser Pro His Lys Asp Trp Ala <210> 140 <211> 72 <212> PRT <213> Cor ynebact er i um gl ut am cum <400> 140 Pro G y Leu Cys Arg n Al a y Arg G y Ser n y Al a Asp Asp 1 5 10 Arg lie G n His Leu Leu Gu Gu His Oy Lys Lys Leu Pro Ala G u 25 Hi s Asn Tyr G y Arg IVet Tyr Lys Leu Pro u Ser IVet G u G u His 40 Phe Lys G u Leu Asp Pro Thr Asn Thr Phe Asn Ala Gy lie Oy Oy 55 Thr Ser Pro His Lys Asp Trp Ala Page 196 ene Sequence 24- 2- 06. txt <210O> 141 <21 1> 1699 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 ODS <222> (1699) <223> FRXA01955 <400> 141 ccat caaaaa at gaacgacc gcggact agc t cggat caag t gacgcgct t gt gat gcaac t gaat at agg aagct tagag gcgacat ccc ctcagcatca c ag O31n gt c ValI ag c Ser ccc Pr o aac As n gga G31y t cg Ser gcg A a gcc A a ggc G31y 1 50 at t I Ie aac As n gga (31y ac c Thr g ac As p ggc G31y ac g Thr ct c Le u ggc G31y c ac Hi s t cg Ser 120 c ac Hi s t cg Ser aag Ly s g ac As p g ac As p 200 act Thr cat Hi s cga Ar g gt c ValI gt c ValI ggc G31y at c I Ie gcg A a cgc Ar g at c I Ie ccg Pr o 1 70 aag Ly s gag (31u t cg Ser gt a ValI t t c Ph e gag O31u at c I Ie 75 t t c Ph e g at As p ggc G31y gag O31u G31y 155 gca A a gt c ValI gt c ValI c ac Hi s ct g Le u ggc G31y at g vb t ccg Pr o c aa O31n gag O31u ac c Thr ccg Pr a 140 ggc G31y tt c Ph e gag O31u gca A a g aa O31u ac c Thr gga G31y 45 t gg Tr p c ag O31n g ac As p gt g ValI ccg Pr a 125 c ac Hi s at c I Ie ac g Thr ct g Le u ct c Le u 205 gcg A a t ct Ser gga G31y cgg Ar g gca A a t ac Tyr c ac Hi s 110 ct g Le u t cg Ser gcg A a cgc Ar g gt c ValI 1 90 g ac As p at c lIe 15 gag O31u cca Pr a gcg A a t cg Ser g at As p 95 ct c Le u ac a Thr gt g ValI aac As n g aa O31u 1 75 aat As n cgt Ar g g at As p cgt Ar g gt c ValI ct g Le u aac As n 80 cgc Ar g at c I Ie c ac Hi s at c I Ie aac As n 1 60 gcg A a c ac Hi s ct a Le u at g vb t 1 gcg A a gcc A a tt c Ph e c ag O31n ac g Thr ccc Pr a aac As n ct g Le u ggg O31y 145 t cg Ser at c I Ie ct g Le u c ag (31n tt c Ph e ac g Thr gcc A a gt a ValI ggc O31y at t I Ie g ac As p ac c Thr 130 t cg Ser ggc O31y tt c Ph e ggc O31y cgc Ar g 210 aag Ly s at g vb t gt g ValI t cc Ser ct g Le u gt g ValI gcg A a 115 g ac As p ac a Thr ggc O31y gcc A a at c I Ie 1 ggc (31y ag a Ar g cca Pr a gt g ValI gt c ValI act Thr at c I Ie 1 00 cgc Ar g gcg A a t ca Ser ag c Ser cgc Ar g 1 t cg Ser gag (31u acg caa cca Th r O31n Pro gga O31y at c Ph e cgc Ar g g ac As p ggt O31y at c I Ie gag O31u ct c Le u at c I Ie c ag O31n 1 gt c ValI ct c Le u t gg Tr p Page 197 Gene Sequence 24-2-06.txt tct Ser t ac Tyr 230 gcg Al a at g let acc Thr cgt Ar g t ac Tyr 310 acc Thr ttc Phe at t SIle ct g Leu gag G u 390 gag G u ttc Phe ttc Phe cac Hi s tgg Tr p 470 gag G u gag G u ccc Pr o ttc Phe ttt Phe 280 ttg Leu ggc Gy gt c Val ttc Phe ccg Pr o 360 aac Asn cac Hi s at g et at c SIle gcg Al a 440 gca Al a tgg Tr p gt c Val ttg Leu t ac Tyr 250 gt g Val at c SIle ct c Leu agt Ser ct g Leu 330 acg Thr ttc Phe ct g Leu ct g Leu aag Lys 410 acg Thr agt Ser ct c Leu gag G u acc cca gct Thr cgc Ar g 235 ct g Leu cgc Ar g ggc G y gaa G u gcc Al a 315 aag Lys tgg Tr p gcc Al a ccc Pr o ct c Leu 395 gag G u tct Ser gcc Al a at c SIle gt g Val 475 Pr o 220 aag Lys ttc Phe acc Thr acg Thr gcc Al a 300 ttc Phe ttc Phe gcc Al a gac Asp aag Lys 380 acc Thr ttc Phe gat Asp gcc Al a ccc Pr o 460 ct g Leu Al a ccc gaa gac Pro u Asp gt g Val gct Al a acc Thr 270 aac Asn at g let ttg Leu agt Ser ggc Gy 350 gt a Val at g bet agc Ser gca Al a gaa G u 430 cgc Ar g gat Asp gag G u cct Pr o t cc Ser 255 ttc Phe acg Thr ccg Pr o gcc Al a cca Pr o 335 ttg Leu tcg Ser at g bet gag G u gag G u 415 aag Lys t ac Tyr gt g Val gag G u tcg Ser 240 ggc G y cct Pr o cac Hi s ct g Leu gag G u 320 gcg Al a ttc Phe caa G n gag G u tcg Ser 400 ccc Pr o agc Ser gcc Al a gcc Al a at c SIle 480 tcg Ser 225 cct Pr o tcg Ser cgc Ar g gag G u cct Pr o 305 aag Lys ct g Leu tcg Ser gcc Al a t ac Tyr 385 cag G n gag G u gcg Al a gcg Al a ct g Leu 465 gac Asp aac gag acc gag Asn G u Thr G u gct Al a gcc Al a gaa G u ct c Leu 290 at c SIle t ac Tyr cag G n aag Lys at g Net 370 cgc Ar g aag Lys cac Hi s tcg Ser ttg Leu 450 cgt Ar g gac Asp t ac Tyr aag Lys 260 cac Hi s gag G u ggt G y aaa Lys cgc Ar g 340 ccc Pr o agc Ser cgt Ar g gcg Al a ggt G y 420 aac Asn cgc Ar g gac Asp ctt Leu 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 Page 198 gtc aag gcg Val Lys Al a tat gtc gcc Tyr Val Al a cag cac ct g G n Hi s Leu 520 t ac Tyr 490 cag G n gag G u cac Hi s gt g Val cgc Ar g Gene Sequence 24- 2- 06. txt ttc ttc tgc cat gtg atg cac cag gac Phe Phe Cys His Val IVet His G n Asp 495 500 gat ctc gag gcg ctg cac gac cgc atc Asp Leu G u Ala Leu His Asp Arg lie 510 515 ggc gcg aag ctg ccc gcc gag cac aac Gy Ala Lys Leu Pro Ala u His Asn 525 530 1603 1651 1699 <210> 142 <211> 533 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 142 Net Thr G n 1 Al a Phe Lys Al a Thr IVet Phe Al a Val G n Val Ser Thr G y Leu Pro li e Val Asn Asp Al a 115 Leu Thr Asp 130 G y Ser Thr 145 Ser G y G y Il e Phe Al a Leu G y Ile 195 G n Arg G y 210 Ser Asn G u 225 Pro Al a Ar g Pro G y 5 Arg Ile Pro Phe Val Arg Val Asp Thr G y lie Ile 100 Arg G u Al a Leu Ser Ile Ser G n 165 Arg Val 180 Ser Leu Su Trp Thr G u Tyr Asn 245 G n Thr Val Ser Pr o Asn 70 Sy Ser Al a Al a Sy 150 I le Asn Sy Ser Ty r 230 Al a Sy Lys Sy Asn Ser Thr l Ie Lys 135 Al a Ar g Asp Asp Pr o 215 Al a Asn Thr Thr Thr 10 Asp G u Hi s 25 G y Tyr Arg 40 Thr Leu Val Leu Il e Val G y Pro G y 90 His Arg Ile 105 Ser Leu Al a 120 His G n Ar g Ser Val I e Lys G y Pro 170 Asp G y Lys 185 Asp Pro u 200 G u Asp Val G u Hi s Leu Pro G u Tyr 250 Ser Val Phe G u SIle Phe Asp Sy G u Sy 155 Al a Val Val Thr Ar g 235 G u Thr Sy Tr p G n Asp Val Pr o 125 Hi s SIle Thr Leu Leu 205 Al a SIle Al a Ser Sy Ar g Al a Tyr Hi s 110 Leu Ser Al a Ar g Val 190 Asp Pr o Val I e G u Pr o Al a Ser Asp Leu Thr Val Asn G u 175 Asn Ar g G u Pr o Leu Phe G u Al a Ser G y 255 Page 199 Se r A a 03 y Leu l'bt Val ene Sequence 24- 2- 06. t xt Phe Ala Val Arg Thr Arg Thr Phe Pro 265 270 Ar g O31u Pr o 305 Ly s Le u Ser A a Tyr 385 O31n (31u A a A a Le u 465 As p ValI Le u Pr o ValI 275 (31u Ser G31y Thr I Ie 355 Ph e As n A a Thr Le u 435 Ly s Ar g O31n Hi s As p 515 aOu Pr o I Ie O31u As p 325 vb t G31y ValI Ph e Ser 405 O31u Ar g Ar g As p O31u 485 As p I Ie As n ValI Ar g 295 vb t Ph e Ser G31y Pr a 375 Hi s Ly s Ph e G31y I Ie 455 As n Ly s ValI Hi s Ph e 280 Le u G31y ValI Ph e Pr a 360 As n Hi s vb t I Ie A a 440 A a Tr p A a A a Le u 520 Tyr Ph e Ar g Ph e Ly s 345 Thr O31n Le u Le u cys 425 A a G31y Le u Tyr Ly s 505 Le u G31y (31u A a 315 Ly s Tr p A a Pr a Le u 395 (31u Ser A a I Ie ValI 475 G31y G31y (31u Thr A a 300 Ph e Ph e A a As p Ly s 380 Thr Ph e As p A a Pr a 460 Le u Hi s ValI Ar g As n 285 As p As p vb t As n Thr 365 Ar g ValI Ph e (31u Thr 445 li1e Pr a Ph e As p G31y 525 Thr Pr a A a Pr a 335 Le u Ser vb t (31u (31u 415 Ly s Tyr ValI (31u cys 495 (31u Ly s Hi s Le u (31u 320 A a Ph e O31n O31u Ser 400 Pr a Ser A a A a I Ie 480 Hi s A a Le u <210O> 143 <21 1> 1035 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1012) <223> RXA00293 <400> 143 agagat t gt t gat t cggcac caact t t acc cacgagggt a gaaat gaagc at t caggat g Page 200 Gene Sequence 24 acaaaaccca acctcacacc aacaacctat cctggagccc -2-06. txt ggt Gy gca Al a caa G n gt g Val at t l Ie cag G n cac Hi s gat Asp gct Al a gca Al a 150 gat Asp gac Asp at c SIle aac Asn tta Leu 230 gaa G u ggc Gy at a l Ie cca Pr o ttt Phe gac Asp gcc Al a acc Thr cag G n 120 gga Gy tct Ser acg Thr gt g Val aaa Lys 200 gct Al a gcc Al a ttg Leu t at Ty r gac Asp aca Thr aat Asn cgc Ar g gca Al a gt g Val aga Ar g ggt Gy gca Al a cct Pr o 170 ttg Leu gag G u gga Gy gaa G u gac Asp 250 cca Pr o t cc Ser gaa G u gcg Al a cgg Ar g 75 gca Al a cgt Ar g tgg Tr p aaa Lys gt a Val 155 at a SIle tgc Cys ctt Leu gaa G u at a SIle 235 gat Asp ct c Leu ct c Leu ttt Phe ggg Gy tgg Tr p at g Met gcc Al a ctt Leu cat Hi s 140 agc Ser t cc Ser gt a Val aaa Lys gt a Val 220 t cc Ser cat Hi s acc Thr gaa G u ccc Pr o 45 ct c Leu t ca Ser gct Al a gat Asp gat Asp 125 ct g Leu agg Ar g aac Asn ccg Pr o gca Al a 205 gt a Val ggg Gy cca Pr o acc Thr aaa Lys ct a Leu aac Asn aat Asn ctt Leu agt Ser 110 ggt Gy gca Al a acc Thr ct g Leu ctt Leu 190 at g Net gat Asp gca Al a ct g Leu aag Lys 15 gcc Al a ctt Leu gca Al a gca Al a ttg Leu 95 tgg Tr p gca Al a gcc Al a ggt Gy cac Hi s 175 acc Thr cag G n aca Thr ggc Gy tgg Tr p 255 gcc Al a gag G u ccc Pr o t at Tyr t ca Ser 80 ct g Leu gca Al a aca Thr at g let aca Thr 160 caa G n gca Al a t cc Ser gaa G u tta Leu 240 ggg G y at g bet 1 ctt Leu ggg G y gac Asp ttc Phe ggg G y gga Gy cca Pr o gtt Val ttg Leu 145 ccc Pr o gt a Val gac Asp act Thr gca Al a 225 gat Asp cgc Ar g aag Lys ttt Phe gga G y aca Thr gt g Val ct g Leu agc Ser gcc Al a 130 aaa Lys acc Thr ctt Leu acc Thr gca Al a 210 tta Leu gt c Val agc Ser gag G u gt c Val gt g Val gct Al a t at Tyr at t SIle act Thr 115 at t l Ie cct Pr o caa G n gcc Al a t at Tyr 195 at t l Ie gtt Val acc Thr aat Asn gca Al a ttc Phe agg Ar g ggg Gy ggc Gy cat Hi s 100 caa G n gt g Val ttt Phe gat Asp gac Asp 180 cat Hi s ttg Leu gac Asp gat Asp gt g Val 260 aaa atc ttt Lys I e Phe ggc Gy act Thr tgg Tr p cag G n caa G n at g bet at a SIe gga Sy ggc Sy ttt Phe 165 gcc Al a ctg Leu at c SIe gcc Al a cct Pr o 245 at c SIe Page 201 ene Sequence 24- 2- 06. t xt at t acc ccg cac gta gcc aac acg t tg acc tcg at g gat cgc at g ct t 931 li1e Thr Pr o Hi s Val Ala As n Thr Leu Thr Ser I'b t As p Ar g I'b t Leu 265 270 275 gcc cca gtg gtg gca gaa aac tac cga cgg ttt ctc gct gga gaa aag 979 Ala Pr o Val Val Ala G31u Asn Tyr Ar g Ar g Phe Leu Ala 31y G31u Lys 280 285 290 at g ct g acc gag gt g gac at c cac aaa ggt t ac t agagcagt g gct t tgaat a 1032 I'b t Leu Thr O31u Val Asp Ilie Hi s Lys O31y Tyr 295 300 tag 1035 <210> 144 <211> 304 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 144 I'bt Lys I I e Phe Val (3 y Phe G3 y Asp Le u As p Ph e O31y O31y Pr o ValI Le u 145 Pr o ValI As p Thr A a 225 As p O31u ValI ValI A a Tyr I Ie Thr 115 li1e Pr o O31n A a Tyr 1 95 li1e ValI Thr O31y Thr Tr p O31n O31n vb t I Ie G31y O31y Ph e 1 65 A a Le u I Ie A a Pr o 245 A a O31n ValI I Ie 70 O31n Hi s As p A a A a 150 As p As p I Ie As n Le u 230 (31u O31u Thr O31n As p ValI Pr o O31n O31y 135 Lys5 A a Hi s O31y ValI 215 As p Pr o I Ie Pr o 40 Ph e As p A a Thr O31n 120 O31y Ser Thr ValI Lys5 200 A a A a Le u ValI O31y Pr o Lys5 O31u vb t 1 05 Thr I Ie Le u O31u ValI 1 85 A a Ar g O31n Pr o Tyr 10 As p Thr As n Ar g A a 90 ValI Ar g O31y A a Pr o 1 70 Le u O31u O31y O31u As p 250 Pr o Ser O31u A a Ar g 75 A a Ar g Tr p Lys5 ValI 1 55 li1e cys Le u Ou le 235 Thr O31u Pr o Le u Ser A a As p As p 125 Le u Ar g As n Pr o A a 205 ValI O31y Thr Lys5 Le u As n As n Le u Ser 110 O31y A a Thr Le u Le u 1 vb t As p A a Lys A a A a 03 u Leu Pro Al a Ty r Al a Ser Leu Leu Tr p Ala Al a Thr Al a [Vb t O3 y Thr 1 Hi s 03 n 1 Thr A a O3 n Ser Thr 03 u O3 y Leu 240 Tr p G31y 255 Asp Hi s Pr o Leu Page 202 Gene Sequence 24-2-06.txt Arg Ser Asn Val Ile IIe Thr Pro His Val Ala Asn Thr Leu Thr Ser 260 265 270 IVet Asp Arg IVet Leu Al a Pro Val Val Ala u Asn Tyr Arg Arg Phe 275 280 285 Leu Ala G y G u Lys IVet Leu Thr G u Val Asp lie His Lys G y Tyr 290 295 300 <210> 145 <211> 687 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (62)..(664) <223> RXN01130 <400> 145 agttcgtggc ggatgctgtg aacgtttccg gtggtcgcgt gggcgaagag gttctgtgtg g atg gat ctg gct cgc aag ctt ggt ctt ctt gct ggc aag ctt gtc gac 109 IVet Asp Leu Ala Arg Lys Leu G y Leu Leu Ala G y Lys Leu Val Asp 1 5 10 gcc Al a gag G u gga G y gaa G u act Thr agc Ser at c SIle aac Asn ggt G1y 145 cca Pr o gt c Val at c SIle cgt Ar g cgt Ar g act Thr cgc Ar g 115 ttc Phe aag Lys t cc Ser gca Al a gag G u ct g Leu gt c Val gtt Val aat Asn cag G n ggt Gy gag G u ggt G y gtt Val at c SIle cag G n gcc Al a cgt Ar g act Thr 135 gct Al a gag G u t cc Ser ttc Phe gt g Val aag Lys act Thr 105 ct g Leu gct Al a at c l Ie gct Al a gct Al a gt c Val aag Lys gt c Val 90 ggt Gy gat Asp cct Pr o aac Asn cga Ar g gtt Val aac Asn acc Thr at t SIle ctt Leu ct g Leu ggt Gy at c SIle 155 ggc G y cgt Ar g gct Al a aac Asn act Thr gag G u cgc Ar g gca Al a 140 gag G u gag G u ggt G y cct Pr o tct Ser ggc Gy cgc Ar g gca Al a 125 ct g Leu gct Al a tct Ser ttc Phe att SIe tct Ser ggc Gy gag G u ggt Gy acc Thr gcg Al a act cag gct gag aag ggt gac ggc gct gtc ctg atc ctg cgt gtt gag Page 203 Thr 03 n A a t cc gct gt c Ser A a Val gct act t cc A a Thr Ser 1 95 Ly s 1 65 g aa (31u c ag O31n As p ct g Le u g at As p ene Sequence 24- 2- 06. txt (31y Ala Val Leu Ilie Leu Arg Val Ou 170 175 gaa gct gaa at c aac gct gag t tg ggt O3 u A a 03 u I I e As n A a 03 u Leu 03 y 185 190 ct t gac t aat tagaga t ccat t tgct t ga Leu Asp 200 <210O> 146 <21 1> 201 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 146 I'bt Asp Leu A a A a (31u G31y (31u Thr Ser I Ie As n G31y 145 Thr Ser A a Pr o ValI lie Ar g Ar g Thr Ar g 115 Ph e Ly s A a ValI Ser 1 Ar g Ser A a (31u Le u ValI ValI As n O31n G31y Ly s 1 65 O31u O31n (31u G31y ValI li1e O31n A a Ar g Thr 135 A a As p Le u As p Lys Leu 03 y Leu O31u Ser Ph e ValI Ly s Thr 1 Le u A a I Ie A a A a 1 As p Le u 10 A a A a ValI Ly s ValI 90 O31y As p Pr o As n ValI 1 70 a u A a Ar g ValI As n Thr li1e Le u Le u O31y 1 Le u I Ie Ly s O31u O31y Pr o Ser O31y Ar g A a 125 Le u A a Le u A a ValI Ser Ph e I Ie Ser O31y O31u O31y Thr A a ValI 1 Le u <210O> <211 <212> <213> <220> <221 <222> <223> 147 326 DNA Cor ynebact er i urn gi ut ani curn ODS (303) FRXA0 1130 Page 204 <400> 147 gtt ggt gcc Val Gy Al a 1 aat ggc cgt Asn G y Ar g cag t ac act G n Tyr Thr ggt gct gct G y Al a Al a aag ggt gac Lys G y Asp gaa gag ct g G u G u Leu cag gtt gat G n Val Asp act Thr 5 ct g Leu gct Al a at c l Ie gct Al a gct Al a gac Asp Gene Sequence 24-2-06.txt ggt ctt gag cgc gtt gag aag atc acc G y Leu G u Arg Val G u Lys Ile Thr 10 gat ctg cgc gca gag ggt ctg aac ctc Asp Leu Arg Ala G u y Leu Asn Leu cct ggt gca ctg ggt acc gtt ggt acc Pro G y Ala Leu G y Thr Val G y Thr 40 aac atc gag gct gct gcg ttg act cag Asn le G u Ala Ala Ala Leu Thr G n 55 gtc ctg atc ctg cgt gtt gag tcc gct Val Leu Ile Leu Arg Val u Ser Ala 70 gaa atc aac gct gag ttg ggt gct act G u Ile Asn Al a G u Leu G y Al a Thr 90 t aat t agaga t ccat t t gct t ga cgc Ar g ttc Phe aag Lys gct Al a gtc Val t cc Ser <210> 148 <211> 101 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 148 Val y Ala Leu Thr G y Leu G u Arg 1 5 Asn y Arg y Leu Asp Leu Arg Al a 25 G n Tyr Thr Asp Ala Pro Gy Ala Leu 40 G y Al a Al a G y I e Asn Ile u Ala Lys y Asp y Ala Val Leu Ile Leu 70 G u G u Leu G u Ala u lie Asn Ala G n Val Asp Leu Asp 100 <210> 149 <211> 604 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(604) <223> RXN03112 G u Sy Thr Al a Val Leu SIle Asn Sy Thr Ser Al a Thr Leu Thr G n Al a Thr Ar g Phe Lys Al a Val Ser Page 205 Gene Sequence 24-2-06.txt <400> 149 gtgagcacgc aatttctttg ctgctgtcta ctgctcgcca gatcctgctg ctgatgcgac gctgcgtgag ggcgagtgga agcggtcttc tttcaacggt gtg gaa att Val G u le aaa Lys cag G n gct Al a gat Asp acc Thr aag Lys gag Su ggt Sy aag Lys gag Su 150 gcg Al a gt c Val ctt Leu cct Pr o ct g Leu gaa G u ggc G y gct Al a gat Asp 120 cct Pr o caa G n gct Al a at c I e gcg Al a cgt Ar g agc Ser gct Al a at c SIle gct Al a t ac Tyr gtt Val ggt Gy gag G u gct Al a tct Ser at g bet at c SIle gcg Al a acc Thr gt g Val 140 ggc Gy acc Thr ct g Leu ttt Phe gat Asp gct Al a gag G u 110 cct Pr o cct Pr o cac Hi s 15 at t SIle aac Asn gt c Val gcg Al a gct Al a 95 t cc Ser tgc Cys cac Hi s at t SIle gtt Val gtt Val acc Thr cac Hi s 80 cgt Ar g ggt G y act Thr ttg Leu cag G n t ac Tyr ttg Leu cac Hi s ctt Leu ggc G y at t SIle tct Ser 130 gct Al a ttg Leu gat Asp gtt Val ctt Leu gct Al a ctt Leu cgt Ar g 115 cct Pr o tct Ser ttc gga Phe G y ttt gct Phe Al a cct tac Pro Tyr gag ttg G u Leu cct aag Pro Lys aag tcc Lys Ser gtt gat Val Asp 100 ggc gct G y Al a ttg ttc Leu Phe act gaa Thr G u gat cgt gcg ggt act gac att gct gat tct gtg ctc Asp Arg Al a Gy Thr Asp lie Ala Asp Ser Val Leu <210> 150 <211> 168 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 150 Val G u IIe Phe G y Lys Thr Val Gy IIe Val Gy Phe G y His IIe 1 5 10 Gy G n Leu Phe Ala G n Arg Leu Ala Ala Phe G u Thr Thr lie Val 25 Ala Tyr Asp Pro Tyr Ala Asn Pro Ala Arg Ala Ala G n Leu Asn Val 40 G u Leu Val G u Leu Asp G u Leu Iet Ser Arg Ser Asp Phe Val Thr Page 206 lIe Le u G31y Hi s As p G31y 145 As p Le u Al a Le u Ar g 115 Pr o Ser ValI Ly s Ser As p Al a Ph e O31u Ly s 1 Ly s Ly s O31n Ph e Le u 135 Al a Le u ene Sequence 24- 2- 06. txt O31u Thr Al a G31y I'bt Phe Asp (3 y O n Il e Il e HIe Asn Al a 90 Al a Leu Al a Asp Al a Il e 03 u 105 110 Asp Val Tyr Ser Thr O31u Pr o 120 125 Pro O31n Val Val Val Thr Pr o 140 O31n Asp Ar g Al a G31y Thr Asp 1 Al a Al a Al a Ser cys Hi s I Ie <210O> 151 <21 1> 649 <212> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 ODS <222> (649) <223> FRXA01133 <400> 151 t gt t t ct agt cgcacgccaa aacccggcgt ggacacgt ct t gt tgt agac ggacat tcct agt tt t tcca ggagt aact t gcagccgacg cggtcgtgcc cgt Ar g gcg Al a cca Pr o t ct Ser aag Ly s gct Al a at t I Ie gt a ValI gga G31y ct g Le u ac c Thr gt c ValI act Thr t cc Ser ct c Le u gca Al a g at As p gt c ValI cgt Ar g gct Al a t gt Cys at c I e gt a ValI gca Al a g at As p gcc Al a 75 ggc G31y gag (31u gcc Al a g aa (31u gt t ValI gct Al a ggc G31y gt c ValI c ac Hi s g at As p gt c ValI aag Ly s 45 g aa (31u gt g ValI at g vb t gca Al a aag Ly s cgt Ar g g aa O31u gt c ValI G31y gt t ValI at t I Ie 110 gcg Al a gt t ValI g ac As p gcc Al a g ac As p aac As n tt g Le u gt g ValI 1 c ag O31n g ac As p gca Al a gct Al a aac As n gca Al a ct g Le u ag c Ser t cc Ser gga O31y ct g Le u gcc Al a gt t ValI ccg Pr o ct g Le u aat As n gt t ValI aac As n gt g ValI aac As n at c I Ie t ct Se r 1 00 act Th r Page 207 cgc Ar g ggt Sy tcg Ser 150 ttg Leu gcg Al a at c ct g I e Leu 120 ctt tca Leu Ser ttg gcc Leu Al a cca cca Pro Pro ct g ct g at g Leu Leu IVet acg gt g t gg Thr Val Tr p 140 aca ttg gt c Thr Leu Val 155 ttg ttg ctt Leu Leu Leu 170 Gene Sequence 24-2-06.txt cga cgc tgc gtg agg gcg agt Arg Arg Cys Val Arg Ala Ser 125 130 aaa ttt tcg gaa aaa ctg tcg Lys Phe Ser G u Lys Leu Ser 145 agt tgt ttg ctc agc gtc ttg Ser Cys Leu Leu Ser Val Leu 160 acg atc ctt acg cta acc ctg Thr Ile Leu Thr Leu Thr Leu 175 gga Gy gta Val ctg Leu ctt Leu 180 <210> 152 <211> 183 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 152 Val Ser G n 1 G n Asp Al a Al a Asn Al a Leu Ar g Lys 145 Ser Leu Thr Pr o Leu Pr o Asp Thr Ser 115 Ser Ser Leu Leu Asn y 5 Val Asp Asn Arg Val Arg Asn Leu Ile Pro Ser Asn 100 Thr Al a G y Ser Val Ser Leu Arg 165 Leu Arg 180 Ar g Al a Pr o Ser Lys 70 Al a SIle Ar g Gy Ser 150 Leu Al a Pr o Leu G u Al a I le Al a Hi s G n Leu 135 Val Ar g Gy Val Leu 10 Asp Al a Leu Asp Thr Val G y Arg G u Al a 90 Al a Cys 105 Leu Leu Ser Thr Al a Thr Pro Leu 170 Ile Ala Asp Lys Leu Ala Val Al a Asp Al a Gy G u Leu Val Leu 155 Leu Val Lys G u Val et Al a Ar g 125 Lys Ser Thr Tr p Al a SIe Leu Al a Ser Cys Ser Leu Leu 175 <210> 153 <211> 1011 <212> DNA Page 208 Gene Sequence 24-2-06.txt <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(988) <223> RXN00871 <400> 153 gggaaaaggc gatcaccagc cgttggctcg acccagcaac ccacggtggc attaacctcg gtttcccaca gaacgattaa ttgaaggaga gcacaggact aag aag ggc gaa ctg gcc cga gat ggt tgg caa Lys Lys Gy G u Leu Ala Arg Asp Gy Trp n 15 acc acc cca ggt tgg gaa tat acc ggc atc cgc Thr Thr Pro y Trp G u Tyr Thr y Ile Arg 30 agt ggt gaa tcg ctt gaa ctg aat gac act ggt Ser y u Ser Leu G u Leu Asn Asp Thr y 45 att cca ctt cag ggc agc ttc gat gtt gcc cac lie Pro Leu G n y Ser Phe Asp Val Ala Hi s 60 cat ctt cac gga aga aag tca gtc ttt gat gga His Leu His G y Arg Lys Ser Val Phe Asp G y 75 tac ctc ccc act gga caa aca gca acg ctc agt Tyr Leu Pro Thr Gy G n Thr Ala Thr Leu Ser 95 gcc gtg gcg gaa gct ccc act cag gaa ccc aag Ala Val Ala G u Ala Pro Thr n G u Pro Lys 105 110 gct cca gca gaa act cct gtg gag ttg cgt gga Ala Pro Ala G u Thr Pro Val G u Leu Arg y 120 125 cga caa gtc cac aac ttt ggc acc ccg gaa gct Arg G n Val His Asn Phe G y Thr Pro G u Al a 135 140 cta atc gtg tgt gaa gta atc acc cca ggt gaa Leu lie Val Cys u Val Ile Thr Pro y u 150 155 160 cct cca cac aag cat gat gag cac atc cca gga Pro Pro His Lys His Asp G u His lie Pro G y 170 175 gag gaa atc tac tac ttc gaa agc gcc cca tcg G u G u Ile Tyr Tyr Phe u Ser Ala Pro Ser 185 190 gcc gaa gca gca gaa gga gct ttc gga atg ttt Ala u Ala Ala u y Ala Phe y Met Phe 200 205 cca gcg ggg gag atc gat atc aac gcc atg gtg Pro Ala G v G u lie Aso lie Asn Ala IVet Val at g Met 1 agc Ser at t SIle gt g Val cat Hi s cca Pr o ggt G y gag G u gct Al a ct c Leu 145 aac Asn cac Hi s cga Ar g t cc Ser cgt tgg ttc cat Arg Trp Phe His gtt Val gcc Al a gaa G u ggt G y acc Thr cag G n tgg Tr p ggc G y 130 gat Asp tgg Tr p gag G u gtt Val acc Thr 210 gt c Val gaa G u cgc Ar g cag G n gat Asp gga G y aag Lys 115 cgc Ar g gct Al a agc Ser t cc Ser ggt Sy 195 t ac Tyr gat Asp ctg Leu at c SIe gtg Val gtg Val cga Ar g 100 t ac Tyr tcg Ser gct Al a tct Ser aag Lys 180 ggc Sy t cc Ser tac agc ggc gat atc Tyr Ser y Asp Ile J I Page 209 Gene Sequence 24-2-06.txt 215 220 225 gcg cta gtt cct ttc gga tac cac ggc cct gcc gtg gca gca cct ggc Ala Leu Val Pro Phe G y Tyr His G y Pro Ala Val Ala Ala Pro G y 230 235 240 245 tat gac ttg tac tac ctc aac gtc atg gca gga cct gat ccg gag aga Tyr Asp Leu Tyr Tyr Leu Asn Val IVet Ala Gy Pro Asp Pro G u Arg 250 255 260 atc tgg ctg att aac gat gac cca gcg cac gcc tgg gtt cga gat aca lie Trp Leu Ile Asn Asp Asp Pro Ala His Ala Trp Val Arg Asp Thr 265 270 275 tgg acc ggg caa gca ttt gat gat cgc ttg cca tat gag aac gca aac Trp Thr Gy G n Ala Phe Asp Asp Arg Leu Pro Tyr G u Asn Ala Asn 280 285 290 aag gag gga taaaatttca tggctgaaac gaa Lys G u G y 295 <210> 154 <211> 296 <212> PRT <213> Corynebacter i um gl utam cum 835 883 931 979 1011 Met 1 Ser SIle Val Hi s Pr o Gy G u Al a Leu 145 Asn Hi s Ar g 0> 154 Arg Trp Phe His Lys Lys G y u Val G u Ar g G n Asp Gy Lys 115 Ar g Al a Ser Ser Gy 195 5 Al a Gy Phe Thr Leu Val SIle Ser Ar g Tyr 165 Leu Ar g Thr Ser SIle Hi s 70 Tyr Al a Al a Ar g Leu 150 Pr o G u Al a Thr Gy Pr o Leu Leu Val Pr o G n 135 I le Pr o G u G u Pr o G u 40 Leu Hi s Pr o Al a Al a 120 Val Val Hi s SIle Al a 200 Gy Ser G n Sy Thr G u 105 G u Hi s Cys Lys Tyr 185 Al a Leu 10 Tr p Leu Sy Ar g Sy 90 Al a Thr Asn G u Hi s 170 Tyr Al a G u G u Ser Lys G n Pr o Pr o Phe Val 155 Asp Phe Asp Thr Asn Asp Val Al a G n G u 125 Thr Thr Hi s Ser Sy Sy Asp Val Phe Thr G u 110 Leu Pr o Pr o SIle Al a 190 Tr p I e Thr Al a Asp Leu Pr o Ar g G u Sy Pr o 175 Pr o Su y Ala Phe y et Phe 205 Page 210 Gene Sequence 24 Ser Thr Tyr Ser Ser Pro Ala Gy G u Ile Asp 210 215 Tyr Ser y Asp lie Al a Leu Val Pro Phe y 225 230 235 Val Ala Ala Pro y Tyr Asp Leu Tyr Tyr Leu 245 250 Pro Asp Pro G u Arg Ile Trp Leu Ile Asn Asp 260 265 Trp Val Arg Asp Thr Trp Thr Gy G n Ala Phe 275 280 Tyr G u Asn Al a Asn Lys G u G y 290 295 <210> 155 <211> 964 <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(964) <223> FRXA00871 <400> 155 gggaaaaggc gatcaccagc cgttggctcg acccagcaac gtttcccaca gaacgattaa ttgaaggaga gcacaggact -2-06. txt Ile Asn Ala IVet Val 220 Tyr His G y Pro Ala 240 Asn Val Net Ala y 255 Asp Pro Ala His Ala 270 Asp Asp Arg Leu Pro 285 ccacggtggc attaacctcg aag Lys acc Thr agt Ser at t SIle cat Hi s t ac Tyr gcc Al a gct Al a ggc Gy cca Pr o gaa G u ctt Leu cac Hi s ccc Pr o gcg Al a gca Al a 120 ct g Leu tgg Tr p ctt Leu ggc Gy aga Ar g gga Gy gct Al a act Thr gcc Al a gaa G u gaa G u agc Ser aag Lys 75 caa G n ccc Pr o cct Pr o cga Ar g t at Tyr ct g Leu ttc Phe t ca Ser aca Thr act Thr gt g Val gat Asp acc Thr aat Asn 45 gat Asp gt c Val gca Al a cag G n gag 15u 125 ggt Gy ggc Gy gac Asp gtt Val ttt Phe acg Thr gaa G u 110 ttg Leu caa G n cgc Ar g ggt Sy cac Hi s gga G y agt Ser aag Lys gga G y at g et 1 agc Ser at t SIle gt g Val cat Hi s cca Pr o ggt G y gag G u gct Al a gtt Val gcc Al a gaa G u ggt G y acc Thr cag G n tgg Tr p ggc 30y 130 cgt tgg ttc cat Arg Trp Phe His gat Asp ctg Leu at c SIe gtg Val gtg Val cga Ar g 100 t ac Tyr tcg Ser Page 211 Gene Sequence 24-2-06.txt cga caa gtc cac aac ttt ggc acc ccg gaa gct ctc Arg G n Val His Asn Phe G y Thr Pro G u Ala Leu 135 140 145 cta atc gtg tgt gaa gta atc acc cca ggt gaa aac Leu lie Val Cys u Val lie Thr Pro Gy Gu Asn 150 155 160 cct cca cac aag cat gat gag cac atc cca gga cac Pro Pro His Lys His Asp G u His lie Pro G y His 170 175 gag gaa atc tac tac ttc gaa agc gcc cca tcg cga G u G u lie Tyr Tyr Phe u Ser Ala Pro Ser Arg 185 190 gcc gaa gca gca gaa gga gct ttc gga atg ttt tcc Ala G u Ala Ala G u G y Ala Phe Gy IVet Phe Ser 200 205 cca gcg ggg gag atc gat atc aac gcc atg gtg tac Pro Ala Gy u Ile Asp Ile Asn Ala Iet Val Tyr 215 220 225 gcg cta gtt cct ttc gga tac cac ggc cct gcc gtg Ala Leu Val Pro Phe G y Tyr His G y Pro Al a Val 230 235 240 tat gac ttg tac tac ctc aac gtc atg gca gga cct Tyr Asp Leu Tyr Tyr Leu Asn Val IVet Ala Gy Pro 250 255 atc tgg ctg att aac gat gac cca gcg cac gcc tgg lie Trp Leu Ile Asn Asp Asp Pro Ala His Ala Trp 265 270 tgg acc ggg caa gca ttt gat gat cgc ttg cca Trp Thr Gy G n Ala Phe Asp Asp Arg Leu Pro 280 285 <210> 156 <211> 288 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 156 IVet Arg Trp Phe His Lys Lys G y G u Leu Ala Arg 1 5 10 Ser Val Val Asp Ala Thr Thr Pro Gy Trp G u Tyr 25 lie Ala u Leu y Ser Gy u Ser Leu u Leu 40 Val G u Arg Ile Phe Ile Pro Leu G n Gy Ser Phe 55 His G y G n Val Thr His Leu His G y Arg Lys Ser 70 Pro Thr Asp Val Leu Tyr Leu Pro Thr Gy G n Thr 90 Gy G n y Arg Val Al a Val Ala G u Ala Pro Thr 100 105 Page 212 gat gct gct cga Asp Ala Ala Arg tgg Tr p gag G u gtt Val acc Thr 210 agc Ser gca Al a gat Asp gtt Val Asp Thr Asn Asp Val Al a G n agc Ser t cc Ser ggt Gy 195 t ac Tyr ggc Gy gca Al a ccg Pr o cga Ar g 275 Gy Gy Asp Val Phe Thr G u 110 tct Ser aag Lys 180 ggc Gy t cc Ser gat Asp cct Pr o gag G u 260 gat Asp Tr p I e Thr Al a Asp Leu Pr o G u Trp Lys 115 Tyr Ile Ala Pro Gene Sequence 24- 2- 06. txt Ala G u Thr Pro Val G u Leu Arg y 120 125 Al a Leu 145 Asn Hi s Ar g Ser Tyr 225 Val Pr o Ar g Al a Ser Ser Gy 195 Tyr Gy Al a Pr o Ser Ar g Tyr 165 Leu Ar g Ser SIle Gy 245 Ar g Ar g Leu 150 Pr o G u Al a Pr o Al a 230 Ty r SIle G n 135 I le Pr o G u G u Al a 215 Leu Asp Tr p Val Hi s Val Cys Hi s Lys Ile Tyr 185 Al a Al a 200 G y G u Val Pr o Leu Tyr Leu II e 265 Phe Val 155 Asp Phe Gy Asp Gy 235 Leu Asp Thr Thr Hi s Ser Phe 205 Asn Hi s Val Pr o Asp 285 Pr o Pr o SIle Al a 190 Gy Al a Gy et Al a 270 G u Gy Pr o 175 Pr o vet vet Pr o Al a 255 Hi s Trp Val Arg 275 Asp Thr Trp Thr G n Ala Phe Asp Arg Leu Pro <210> 157 <211> 373 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(373) <223> RXN02829 <400> 157 tttttcgttt aatctcatat ttaaacacgt tccttttaat ctgaat t cgt cagt t aaagt gtatcgaaag gagactggac ct a Leu tta Leu gat Asp gtt Val agt Ser aca Thr ggt Gy gaa G u at c I e t at Tyr acg Thr at t SIle gaa G u aaa Lys t at Tyr gtt Val at t SIle aaa Lys ttt Phe ggt Gy tct Ser gcg Al a gat Asp 45 aaa Lys ctt ggg Leu G y 15 cca att Pro Ile gca gat Al a Asp tta aaa Leu Lys Page 213 t ggt t t t at a aatt gat aaa atg caa aaa aat att IVet n Lys Asn Ile 1 tta ggt tgc atg agt Leu y Cys IVet Ser att gaa agt gca att Ile u Ser Ala l e att tac gat caa gga I le Tyr Asp n G y aaa tat caa aat cgt Lys Tyr G n Asn Arg Gene Sequence 24- 2- 06. txt 60 gat gac atc gtt atc gga act aaa gtt gga aat cga tta act gac gat Asp Asp Ile Val I le y Thr Lys Val y Asn Arg Leu Thr Asp Asp 75 80 gga cat atg acg tgg gat G y His IVet Thr Trp Asp <210> 158 <211> 91 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 158 IVet G n Lys Asn Ile Leu Lys Ser y 1 5 Leu y Cys IVet Ser Leu y Thr Asp 25 I le u Ser Al a li e Asp Asn G y li e 40 lie Tyr Asp n y Val Asn u u 55 Lys Tyr G n Asn Arg Asp Asp lie Val 70 Arg Leu Thr Asp Asp G y His IVet Thr <210> 159 <211> 376 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(376) <223> FRXA02829 <400> 159 tttttcgttt aatctcatat ttaaacacgt tcc ctgaattcgt cagttaaagt gtatcgaaag gag G u I e Ser G u Leu G y Lys Lys Ala G n Pro lie Tyr Phe Asp Thr Ala Asp Val y Lys Ala Leu Lys Sy Thr Lys Val y Asn 75 Asp ttttaat tggttttata aat t gataaa lactggac atg caa aaa aat att IVet n Lys Asn Ile 1 ctt ggg tta ggt tgc atg agt Leu G y Leu y Cys Iet Ser 15 cca att att gaa agt gca att Pro lie lie u Ser Ala lie gca gat att tac gat caa gga Ala Asp Ile Tyr Asp n G y tta aaa aaa tat caa aat cgt Leu Lys Lys Tyr G n Asn Arg Page 214 ct a Leu tta Leu gat Asp gtt Val agt Ser aca Thr ggt Sy gaa G u at c I e t at Tyr acg Thr at t SIle gaa G u aaa Lys t at Tyr gtt Val at t SIle aaa Lys ttt Phe ggt Gy tct Ser gcg Al a gat Asp 45 aaa Lys Gene Seq gat gac atc gtt atc gga act aaa gtt Asp Asp Ile Val Ile G y Thr Lys Val 75 gga cat atg acg tgg gga tcc G y His Mbt Thr Tr p y Ser <210> 160 <211> 92 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 160 IVet G n Lys Asn I e Leu Lys Ser G y 1 5 Leu y Cys IVet Ser Leu y Thr Asp 25 I le u Ser Al a lI e Asp Asn G y li e 40 I e Tyr Asp Gn G y Val Asn Gu G u 55 Lys Tyr G n Asn Arg Asp Asp lie Val 70 Arg Leu Thr Asp Asp G y His IVet Thr luence 24-2-06. t xt gga aat cga tta act gac gat Sy Asn Arg Leu Thr Asp Asp 80 G u Lys Tyr Val Gy 75 Gy I le Ser G u Leu G y Lys Ala n Pro lie Phe Asp Thr Ala Asp G y Lys Al a Leu Lys Thr Lys Val G y Asn Ser <210> 161 <211> 948 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(925) <223> RXN01468 <400> 161 tgccaaggat ttgaccaccg tgcaggattt gattgacttt ttagcgggaa aatttcgccc aaaacaggga caatggtgtt t ca Ser at t SIle gag G u tct Ser ct g Leu ggt Gy at g Net cgt Ar g gac Asp gat Asp ttg Leu aag Lys gac Asp ttt Phe aac Asn t cc Ser gt c Val cag G n t cc Ser ttg Leu ct c at c Leu II e 15 tct ct c Ser Leu att ttc Ile Phe gat at c Asp Il e Page 21 at t aacacca at aaggct ga atg aca gtg aac att IVet Thr Val Asn Ile 1 aaa gag ggc gag ata Lys G u G y G u Ile acc gat aac aat gtg Thr Asp Asn Asn Val acc ccg agg gat ctt Thr Pro Arg Asp Leu ccg ccg gag cgt att Pro Pro G u Arg lie Gene Sequence 24-2-06.txt tgg Tr p gag G u cat Hi s ct g Leu aat Asn act Thr 150 gca Al a cca Pr o t cc Ser tct Ser t cc Ser 230 at c SIle gac Asp act tct gca acc gcc act Thr Ser Ala Thr Al a Thr aca Thr gcg Al a gaa G u 120 at t SIle cct Pr o at t l Ie cct Pr o cac Hi s 200 ttg Leu gac Asp t cc Ser gca Al a t at Ty r tgg Tr p cgc Ar g ggt Gy cca Pr o gca Al a 170 at g Met gt c Val gcc Al a gag G u gcc Al a 250 cac Hi s 75 gtt Val at t SIle aca Thr ggc Gy agt Ser 155 gct Al a at g bet at g bet ggc Gy at c SIle 235 gat Asp gt a Val gtt Val ttg Leu t at Tyr gct Al a 140 ggc Gy act Thr cgc Ar g at c SIle ct g Leu 220 cgc Ar g ctt Leu cca Pr o gct Al a ggc Gy acg Thr t cc Ser 125 cgc Ar g at t SIle ggc Gy agt Ser ggc Gy 205 agc Ser cgc Ar g gcc Al a gat Asp cac ttc ctg aaa tcc His Phe Leu Lys Ser gag G u gat Asp 110 ttc Phe ttt Phe ttg Leu gct Al a gcg Al a 190 gac Asp acc Thr t ac Tyr gat Asp gag G0u 270 t cc Ser 95 gca Al a gag G u at t SIle cct Pr o gag Gu 175 ct g Leu cgc Ar g gt g Val ccc Pr o tgc Cys 255 cag G n ggt Gy aat Asn gca Al a tgc Cys gct Al a 160 cct Pr o aac Asn at g et ct g Leu ttc Phe 240 tgg Tr p cag G n acc Thr gag G u act Thr 130 aac Asn ggc Gy t ac Tyr at c SIle acc Thr 210 cga Ar g cca Pr o gat Asp act Thr cag gtc aag G n Val Lys act gcg ttg Thr Al a Leu 100 ttt gtt gtc Phe Val Val 115 act gcg at a Thr Ala lle ccg gat gtc Pro Asp Val tct gtc gcc Ser Val Al a 165 atc ggc aag I e G y Lys 180 ggg gcg cat G y Al a Hi s 195 gac gtg aaa Asp Val Lys agc gga att Ser G y Ile act cac gt g Thr Hi s Val 245 cct ttc ggt Pro Phe G y 260 tagtattctg taggtcatgg cat <210> 162 <211> 275 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 162 IVet Thr Val Asn Ile Ser Tyr Leu Thr Asp IVet Asp y Val Leu Ile 1 5 10 Lys Gu Gy Gu lie lie Pro Gy Ala Asp Arg Phe Leu n Ser Leu 25 Thr Asp Asn Asn Val G u Phe IVet Val Leu Thr Asn Asn Ser Ile Phe Page 216 Gene Sequence 24-2-06.txt Arg Leu Lys Thr Sy Leu Asp Ile Thr Pr o Lys Leu Pr o SIle Thr 145 Thr Tyr Thr Asp Val 225 Ar g Asp Phe Arg Asp G u Ar g G n Val Thr Al a 100 Phe Val 115 Thr Al a Pro Asp Ser Val I e G y 180 G y Al a 195 Asp Val Ser G y Thr His Pro Phe 260 Asp 275 Leu SIle Lys Leu Val l Ie Val Al a 165 Lys Hi s Lys SIle Val 245 Al a Thr Sy Thr Sy Leu 135 Sy Leu Asn G u Sy 215 Asp Asn Al a Al a Sy 105 Thr Leu Ser Thr Val 185 Thr G u Al a SIle Phe 265 Thr Tyr 90 Tr p Ar g Sy Pr o Al a 170 et Val Al a G u Al a 250 Al a 75 Val SIle Thr Sy Ser 155 Al a bet et Gy I e 235 Asp Al a Sy Thr Ser 125 Ar g SIle Sy Ser Sy 205 Ser Ar g Al a Hi s G u Asp 110 Phe Phe Leu Al a Al a 190 Asp Thr Tyr Asp Phe Ser Al a G u SIle Pr o G u 175 Leu Ar g Val Pr o Cys 255 Leu Sy Asn Al a Cys Al a 160 Pr o Asn vet Leu Phe 240 Tr p Sy Asp y Ala His Val Pro Asp Su Gn n 270 <210> 163 <211> 948 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(925) <223> FRXA01468 <400> 163 tgccaaggat ttgaccaccg tgcaggattt gattgacttt attaacacca ataaggctga ttagcgggaa aatttcgccc aaaacaggga caatggtgtt atg aca gtg aac att 115 IVet Thr Val Asn Ile 1 tca tat ctg acc gac atg gac ggc gtc ctc atc aaa gag ggc gag ata 163 Ser Tyr Leu Thr Asp Iet Asp G y Val Leu Ile Lys G u G y G u lie 15 Page 217 Gene Sequence 24-2-06.txt at t SIle gag G u tct Ser tgg Tr p gag G u cat Hi s ct g Leu aat Asn act Thr 150 gca Al a cca Pr o t cc Ser tct Ser t cc Ser 230 at c SIle gac Asp ggt Gy at g bet cgt Ar g tct Ser aca Thr gcg Al a gaa Gu 120 at t SIle cct Pr o at t l Ie cct Pr o cac Hi s 200 ttg Leu gac Asp t cc Ser gca Al a gca gat cgt ttt Ala Asp Arg Phe gtt ttg acc aac Val Leu Thr Asn ctt aag act tcc Leu Lys Thr Ser gca acc gcc act Ala Thr Al a Thr 75 gcc tat gtt gtt Ala Tyr Val Val ggt tgg att ttg Gy Trp lie Leu 105 acc cgc aca tat Thr Arg Thr Tyr ttg ggt ggc gct Leu G y G y Al a 140 tca cca agt ggc Ser Pro Ser y 155 acc gca gct act Thr Ala Al a Thr 170 gtg atg atg cgc Val Met et Arg 185 act gtc atg atc Thr Val et Ile gaa gcc ggc ctg Su Al a G y Leu 220 gcc gag atc cgc Ala u lie Ar g 235 atc gcc gat ctt Ile Al a Asp Leu 250 ttt cac gta cca Phe His Val Pr o 265 ctt cag tct ctc acc Leu G n Ser Leu Thr gat Asp aac aat gt g Asn Asn Val aac Asn 45 ggt G y gct Al a ggc G y acg Thr t cc Ser 125 cgc Ar g at t SIle ggc Gy agt Ser ggc G y 205 agc Ser cgc Ar g gcc Al a gat Asp t cc Ser ttg Leu cac Hi s gag G u gat Asp 110 ttc Phe ttt Phe ttg Leu gct Al a gcg Al a 190 gac Asp acc Thr t ac Tyr gat Asp gag G u 270 ttc Phe at c SIle ct g Leu 80 ggt G y aat Asn gca Al a tgc Cys gct Al a 160 cct Pr o aac Asn at g bet ct g Leu ttc Phe 240 tgg Tr p cag G n acc Thr ccg Pr o aaa Lys ct g Leu cct Pr o at c l Ie acc Thr 145 act Thr t at Tyr acc Thr gac Asp gtt Val 225 cgc Ar g gac Asp ttc Phe ccg Pr o ccg Pr o t cc Ser acc Thr gag G u act Thr 130 aac Asn ggc Gy t ac Tyr at c SIle acc Thr 210 cga Ar g cca Pr o gat Asp act Thr gat Asp cgt Ar g gt c Val gcg Al a 100 gtt Val gcg Al a gat Asp gt c Val ggc Gy 180 gcg Al a gt g Val gga G y cac Hi s ttc Phe 260 ctt Leu att SIe aag Lys ttg Leu gtc Val at a SIe gtc Val gcc Al a 165 aag Lys cat Hi s aaa Lys att SIe gtg Val 245 ggt G y tagtattctg taggtcatgg cat Page 218 21> 14 ene Sequence 24- 2- 06. txt <211> 275 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm curn <400> 164 [Vbt Thr Val Asn I Ie Ser Tyr Leu Thr Ly s Thr Thr Pr o Ly s Le u Pr o I Ie Thr 145 Thr Tyr Thr As p ValI 225 Ar g As p Ph e O31y (1u Asn Asn Ar g As p O31u Ar g G3 n Val Thr A a 1 00 Phe Val 115 Thr A a Pr o As p Ser Val I Ie G31y 1 80 a3 y A a 1 95 As p Val Ser G3 y Thr Hi s Pr o Phe 260 As p 275 le ValI Le u I Ie Lys5 Le u ValI I Ie ValI A a 1 65 Lys5 Hi s Lys5 I Ie ValI 245 I Ie (31u Ser Tr p 70 (31u Hi s Le u As n Thr 150 A a Pr o Ser Ser Ser 230 li1e Pr a Ph e A a Thr G31y Thr G31y Le u 135 G31y Le u As n (31u G31y 215 As p As n G31y vb t 40 Ar g Ser Thr A a (31u 120 li1e Pr a I Ie Pr a Hi s 200 Le u As p Ser A a 25 ValI Le u A a A a G31y 1 05 Thr Le u Ser Thr ValI 1 85 Thr (31u A a I Ie As p 10 As p Le u Lys5 Thr Tyr 90 Tr p Ar g G31y Pr a A a 1 70 vb t ValI A a (31u A a 250 vb t Ar g Thr Thr A a ValI I Ie Thr G31y Ser 1 A a vb t vb t Gy le 235 As p As p Ph e As n Ser Thr ValI Le u Tyr A a 140 G31y Thr Ar g I Ie Le u 220 Ar g Le u G31y Le u As n G31y A a G31y Thr Ser 125 Ar g I Ie G31y Ser G31y 205 Ser Ar g A a ValI O31n Ser Le u Hi s (31u As p 110 Ph e Ph e Le u A a A a 1 As p Thr Tyr As p Le u Ser I Ie As p Ph e Ser A a Ou le Pr a (31u 1 Le u Ar g ValI Pr a cys 255 Gy AspGy Ala Phe His Val Pro Asp Olu On On 265 270 <210O> 165 <21 1> 1128 <212> DNA <213> Cor ynebact er i urn gi ut anm curn <220> <221 ODS Page 219 ene Sequence 24- 2- 06. t xt <222> (101)..(1105) <223> RXA00794 <400> 165 gcgggttgat acagcccaag cgccgataca tttataatgc cgtaaccagg tcagatcaag tgccccagga ggcccttcag gcctagatac gtgcaaccca ccc Pr o gaa (31u aat Asn t ca Ser gaa (31u cct Pr o gct Al a ggc G31y gt g Val cac Hi s 150 gt c Val gac Asp gt t Val at g Ivb t at g Ivb t acg Thr gct Al a ggc G31y acc Thr cca Pr o gt t Val ggt G31y at g Ivb t 120 cct Pr a at c l Ie gt t Val cgt Ar g ggt G31y 200 ggc G31y t gc Cys gac Asp ct g Leu ggt G31y aag Lys ct g Leu at c l Ie ccc Pr a gat Asp gcc Al a gcg Al a gt g Val 170 gca Al a gt t Val ggc gy ggt (31y cgt Ar g gct Al a gcc Al a ggc G31y t ac Tyr 75 gca Al a aac Asn cca Pr a gca Al a gt g Val 155 ct t Leu ggc G31y gca Al a gga gy (31y aac Asn t ct Ser gct Al a gt c Val aac Asn gt t Val gca Al a t cc Ser ggc G31y 140 gca Al a gac Asp gca Al a gca Al a acc Thr 220 gaa (31u ct t Leu gga G31y gt t Val 45 gt t Val ggc G31y gac Asp at t l Ie t cc Ser 125 aag Lys aag Lys cgt Ar g aag Lys gct Al a 205 cca Pr a at c l Ie gct atg Al a [Vb t 15 cgt tgg Arg Trp gac gcc Asp Al a gtt atc Val I e gaa gag cca gtt Pr o Val 95 tcc att Ser lIe 110 gtc ttc Val Phe atc gac I Ie Asp tcc aag Ser Lys cct cgc Pr o Ar g 175 gtt cgt Val Arg 190 cag gat 3n Asp gaa ggc cag ggc (31n (1y gag G31u gt t Val at g Ivb t ggc G31y gt c Val 80 gac Asp ct C Leu t ac Tyr at c l Ie gga G31y 160 cac Hi s ct C Leu t cc Ser at c l Ie at g Ivb t 1 ct g Leu gga G31y cgc Ar g gag G31u gga G31y ggc G31y gca Al a at g Ivb t gaa G31u 145 at c l Ie at c l Ie at c l Ie aac Asn at c l Ie 225 gt g Val cgt Ar g cag O31n ggc G31y acc Thr acc Thr gct Al a aag Lys 130 gct Al a aac Asn gaa O31u t cc Ser t cc Ser 210 act Thr cga Ar g ggc G31y ct c Leu gaa O31u ggc G31y acc Thr gca Al a 115 aag Lys cca Pr a cct Pr a ct g Leu gac Asp 195 gt g Val gcg Al a aac cta aag aac Asn Leu Lys Asn gt t Val at g Ivb t at c l Ie aaa Lys ttt Phe ct g Leu 100 gag O31u at c l Ie gt t Val t cc Ser at c l Ie 180 ggc G31y gac Asp t gc Cys acg Thr aag Lys aac Asn gac Asp gga G31y at g rvb t cgt Ar g gcc Al a gcc Al a gac Asp 165 gca Al a gac Asp at c l Ie gcc Al a atc ctg gcc cca atg aac Ile Leu Ala Pro I'bt Asn Page 220 230 235 gat ttc gag cgc cag aag gca cac g Asp Phe O31u Arg O31n Lys Ala Hi sA 250 gt t ct g cac acc aac gat ct g gt g a Val Leu Hi s Thr Asn Asp Leu Val S 265 2 gca acc ggt gt g acc aac ggt gac a A a Thr 03 y Val Thr Asn 03 y Asp 280 285 gca aac ggc gca acc acc cgt tcc c Ala Asn G31y Ala Thr Thr Ar g Ser L 295 300 acc atc cgc cac atc gag tct gtc c Thr Ilie Ar g His Ilie O31u Ser Val F- 310 315 t ac t cc gt g gt t gac t ac acc acc g Tyr Ser Val Val Asp Tyr Thr Thr P 330 aac <210> 166 <211> 335 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn Sequence 24-2- 06. txt 240 245 iac gct ggt ct g gt t ct t gat cag osp Ala G31y Leu Val Leu Asp O31n 255 260 .gc t cc gac aac t gc tac t tc gt g ier Ser Asp Asn Cys Tyr Phe Val 70 275 .tg ct c cgt ggc gt t t cc t ac cgc /bt Leu Ar g G31y Val Ser Tyr Ar g 290 tg gtt atg cgc gca aag tca ggc .eu Val I'b t Ar g Ala Lys Ser G31y 305 ac cag ct g t cc aag ct g cag gaa fis O31n Leu Ser Lys Leu O31n O31u 320 325 icg acc t aagagct ct t agt t cgaaa Il a Thr 335 883 931 979 1 027 1 075 1125 1128 <400> 166 vb t Le u O31y Ar g O31u O31y O31y A a vb t O31u 145 li1e Asn Leu Lys Asn Pro O31u Thr Val Ar g Val Ar g 31y [Vb t O3 n Leu I I e O3 y 03 u Lys Thr 03 y Phe Thr Thr Leu 1 00 A a A a 03 u 115 Lys Lys I I e 130 Ala Pr o Val As n Pr o Ser Thr Lys5 As n As p O31y vb t Ar g A a A a As p 1 65 A a O31u ValI 55 A a O31u O31u Thr O31y 135 As n Thr A a O31y 40 Thr Pr o ValI O31y vb t 120 Pr o I Ie ValI Pr o Asp 10 A a Leu 25 As p (1y I'b t Lys I'b t Leu As p I I e 90 Ar g Pr o 1 05 Ty r As p O3 u A a As n A a Val Val 1 70 Ar g A a A a O31y Tyr 75 A a As n Pr o A a ValI 1 55 As n Ser A a ValI As n ValI A a Ser O31y 140 A a Le u O31y ValI ValI O31y As p I Ie Ser 125 Lys5 Lys5 A a Ar g As p ValI O31u Pr o Ser 110 ValI I Ie Ser [vb t (1u Tr p Val A a [Vb t I Ie O31y O3 u Val Val As p 1 1 e Leu Phe Tyr As p I I e Lys 03 y 1 Ar g Hi s 1 Leu Asp Arg Pro Page 221 I e I e Asn SIle 225 Leu Leu Asn Sy Ar g 305 Ser Leu Asp 195 Val Al a Pr o Leu Tyr 275 Ser Lys Leu Al a Asp SIle Al a Asn 245 G n Val Ar g Gy G u 325 Asp Val bet Met 230 Asp Val Al a Al a Thr 310 Tyr SIle Al a bet 215 Lys Phe Leu Thr Asn 295 I le Ser Gene Sequence 24-2-06.txt Arg Arg Ala Gy Ala Lys Val 185 190 Sy Ala Val Ala Ala Ala G n 200 205 Sy Thr Gy y Thr Pro u 220 Cys Mvt Gy Gy G u Ile n 235 G u Arg G n Lys Ala His Asp 250 His Thr Asn Asp Leu Val Ser 265 270 G y Val Thr Asn G y Asp IVet 280 285 Sy Ala Thr Thr Arg Ser Leu 300 Arg His Ile u Ser Val Hi s 315 Val Val Asp Tyr Thr Thr Al a 330 Ar g Asp Gy Gy Al a 255 Ser Leu Val G n Thr 335 <210> 167 <211> 1035 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1012) <223> RXN02920 <400> 167 tgcatgcaga ttatctgtcc aactacgcca gccgcgcgt a cgggtggcgt cgaaaagcat ttttaaagga gtttaagacg t at Tyr cat Hi s ggt Gy cag G n aat Asn cat Hi s cgg Ar g gcg Al a t cc Ser aag Lys tgg Tr p gag G u gag G u gcg Al a cgt Ar g t cc Ser at t SIle ccg Pr o at t l Ie gca Al a acg Thr aaa Lys gat Asp 45 gat Asp aac Asn acc Thr gat Asp ttg Leu gcg Al a gcg Al a gct gtc Al a Val 15 gca gac Al a Asp ccg gag Pro G u ctg gt c Leu Val gct ggc Al a G y 80 Page 222 aagcgcgggc ctgctggtgg atg aag ttt gtt atg et Lys Phe Val bet 1 att gag ggt ggc gga lie Gu Gy Gy y ttc att ttc ttt aat Phe Ile Phe Phe Asn aac atc aag ttc gtg Asn Ile Lys Phe Val aag cgt ggt gtc gtc Lys Arg y Val Val ctg tac gct gac acc Leu Tyr Ala Asp Thr Gene Sequence 24-2-06.txt gtt gct gag tcc acc att ggt Val Ala u Ser Thr Ile Gy tta att ct g Leu IIe Leu gcg cag atg cac atg cat Ala n Met His et His 100 gcg Al a aac Asn gcc Al a gt g Val 150 gat Asp gat Asp gt c Val aat Asn ttg Leu 230 gag G u at c SIle ggc Gy act Thr aag Lys 120 ggc Gy acc Thr acc Thr ttt Phe gca Al a 200 ggg Gy aac Asn ctt Leu cct Pr o ct c Leu 280 ttg Leu tgg Tr p ggc Gy gcg Al a gcc Al a 170 ct c Leu act Thr ggc Gy acc Thr gac Asp 250 act Thr ttg Leu gct Al a ct g Leu gt g Val gtt Val 155 at g bet at c SIle tt g Leu ccg Pr o at t SIle 235 agc Ser gca Al a cgc Ar g aag Lys cat Hi s cgt Ar g 140 aat Asn gat Asp ct g Leu ggc Gy ct g Leu 220 gcg Al a cac Hi s aac Asn aac Asn tgg Tr p 110 aat Asn ct g Leu tct Ser gct Al a ct g Leu 190 at g et aac Asn gct Al a ct g Leu aat Asn 270 gag Gu agc Ser aaa Lys gaa Gu ggt Gy gag Gu 175 act Thr aag Lys acc Thr gcg Al a tgg Tr p 255 gag G u ttg Leu gt g Val act Thr at g Net cgt Ar g 160 cac Hi s gat Asp cct Pr o gat Asp ct g Leu 240 gag G u agg Ar g ttt Phe cgg Ar g gt c Val ct c Leu 145 ccg Pr o gt g Val gcc Al a tct Ser gat Asp 225 gac Asp at g Net at t SIle gag Gu cct Pr o gct Al a 130 aag Lys gt g Val tgg Tr p act Thr gcc Al a 210 ct g Leu gtt Val gac Asp cgt Ar g gca Al a gag G u 115 at t l Ie ccg Pr o gaa Gu gct Al a t at Tyr 195 gt g Val gt g Val acc Thr aat Asn gct Al a 275 ggc Gy gt g Val ttg Leu ttc Phe ggt Gy gag Gu 180 cag G n gt g Val gat Asp gat Asp gt g Val 260 ttg Leu gag Gu gaa G u ggc Gy aac Asn gca Al a 165 gct Al a at c SIe gtc Val gca Al a cct Pr o 245 gtt Val acc Thr cag G n 290 atg gcc acc gag gtc gat gtg gtg gct ggc tac taggcctttt atggtgtgat 1032 Net Ala Thr G u Val Asp Val Val Ala y Tyr 295 300 ccg 1035 <210> 168 <211> 304 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 168 IVet Lys Phe Val IVet Tyr Pro His Leu Trp u Ser Thr Thr Ala Val 1 5 10 Page 223 lie u G y G y Hi s G u Gene Sequence 24-2-06.txt Arg Val u Asp Ile Lys Asp Ala Asp Phe Asn Lys Leu G n Ar g Val Leu 145 Pr o Val Al a Ser Asp 225 Asp vet SIle G u Phe Lys Gy Al a Hi s G u 115 I le Pr o G u Al a Tyr 195 Val Val Thr Asn Al a 275 Gy Asn Val Val Thr Hi s G u Gy Asn Al a 165 Al a SIle Val Al a Pr o 245 Val Thr G n Gy G n Asn 70 Val Al a Asn Al a Val 150 Asp Asp Val Asn Leu 230 G u SIle Gy bet Ser Al a G u Al a Thr Asn Gy 135 Lys G u Val Asn Val 215 Asn Pr o Thr G u Al a 295 Al a 40 Ser Lys G u Thr Lys 120 Gy Thr Thr Phe Al a 200 Gy Asn Leu Pr o Leu 280 Thr Pr o bet Al a Ser Ar g 105 Ser SIle SIle Phe Val 185 G u Ar g Gy Pr o Hi s 265 Thr G u G u Al a Ar g Thr 90 Leu Tr p Gy Al a Al a 170 Leu Thr Gy Thr Asp 250 Thr Leu Val Phe Gy Tr p I le Al a Leu Val Val 155 et SIle Leu Pr o SIle 235 Ser Al a Ar g Asp Asp Asp Asn Leu Ser Asp 125 Leu Asn Lys Pr o Lys 205 I le Gy Pr o Thr SIle 285 Val Leu Al a Al a SIle Tr p 110 Asn Leu Ser Al a Leu 190 bet Asn Al a Leu Asn 270 G u Al a Pr o Leu Al a Leu Ser Lys G u Gy G u 175 Thr Lys Thr Al a Tr p 255 G u Leu Gy <210> 169 <211> 779 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (52)..(756) <223> FRXA02379 Page 224 Gene Sequence 24-2-06.txt <400> 169 tgcaggcctc catggcgggt attgatgcgc tggtcaagcg tggtgtcgtc a atg aga IVet Arg 1 aag Lys gag G u act Thr aag Lys ggc G y acc Thr acc Thr ttt Phe 115 gca Al a ggg Gy aac Asn ctt Leu cct Pr o 195 ct c Leu acc Thr cgt Ar g acc Thr ttg Leu tgg Tr p ggc G y gcg Al a gcc Al a ct c Leu act Thr ggc Gy acc Thr 165 gac Asp act Thr ttg Leu gt c Val gca Al a ggt Sy aag Lys cat Hi s cgt Ar g aat Asn gat Asp ct g Leu ggc Gy 135 ct g Leu gcg Al a cac Hi s aac Asn aac Asn 215 aac Asn tta Leu tcg Ser 40 gac Asp ct g Leu aac Asn aag Lys ccg Pr o 120 aag Lys at c SIle ggt G y ccg Pr o acg Thr 200 at t SIle gcg Al a at t SIle tgg Tr p aat Asn ct g Leu tct Ser gct Al a 105 ct g Leu at g bet aac Asn gct Al a ct g Leu 185 aat Asn gag G u gct Al a 10 ct g Leu agc Ser aaa Lys gaa G u ggt Gy 90 gag G u act Thr aag Lys acc Thr gcg Al a 170 tgg Tr p gag G u ttg Leu ggc G y gcg Al a gt g Val act Thr at g et cgt Ar g cac Hi s gat Asp cct Pr o gat Asp 155 ct g Leu gag G u agg Ar g ttt Phe t ac Tyr 235 ctg tac gct gac acc gtt gct Leu Tyr Ala Asp Thr Val Ala cag atg cac atg cat gcg acg Sn et His Net His Ala Thr cgg cct gag gtg gaa aac aac Arg Pro G u Val G u Asn Asn 45 gtc gct att ttg ggc gcc ggt Val Ala lle Leu y Ala y 60 ctc aag ccg ttc aac gtg aag Leu Lys Pro Phe Asn Val Lys ccg gtg gaa ggt gca gat gaa Pro Val Gu Gy Ala Asp u gtg tgg gct gag gct gat gtg Val Trp Ala G u Ala Asp Val 110 gcc act tat cag atc gtc aat Ala Thr Tyr n Ile Val Asn 125 130 tct gcc gtg gtg gtc aat gtg Ser Ala Val Val Val Asn Val 140 145 gat ctg gtg gat gca ttg aac Asp Leu Val Asp Ala Leu Asn 160 gac gtt acc gat cct gag cca Asp Val Thr Asp Pro G u Pro 175 atg gac aat gtg gtt atc act IVet Asp Asn Val Val Ile Thr 190 att cgt gct ttg acc ggc gaa lie Arg Ala Leu Thr Gy u 205 210 gag gca ggc gag cag atg gcc Su Ala G y G u G n Mvt Ala 220 225 taggcctttt atggtgtgat ccg gtg gtg gct ggc Val Val Ala y <210> 170 <211> 235 Page 225 Gene Sequence 24-2-06.txt <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 170 IVet Arg Lys 1 Val Ala u Al a Thr Thr Asn Asn Lys Al a G y G y Val Lys Thr Asp G u Thr Asp Val Phe 115 Val Asn Ala 130 Asn Val y 145 Leu Asn Asn G u Pro Leu I e Thr Pro 195 G y G u Leu 210 et Al a Thr 225 <210> 171 <211> 792 <212> DNA His Ar g 5 Ser Thr Arg Leu Ser Trp I e G y I e Al a Phe Al a 100 Val Leu G u Thr Arg G y G y Thr 165 Pro Asp 180 Hi s Thr Thr Leu G u Val Trp Ala Asn Ala SIle Al a Leu Val 70 Val bet SIle Leu Pr o 150 I le Ser Al a Ar g Asp 230 Sy Lys Hi s 55 Ar g Asn Asp Leu Sy 135 Leu Al a Hi s Asn Asn 215 Val I e 25 Tr p Asn Leu Ser Al a 105 Leu bet Asn Al a Leu 185 Asn a u Al a Al a 10 Leu Ser Lys G u Sy 90 G u Thr Lys Thr Al a 170 Tr p G u Leu Sy Sy Al a Val Thr bet 75 Ar g Hi s Asp Pr o Asp 155 Leu G u Ar g Phe Tyr 235 Leu G n Ar g Val Leu Pr o Val Al a Ser 140 Asp Asp Met SIle G u 220 Tyr bet Pr o Al a Lys Val Tr p Thr 125 Al a Leu Val Asp Ar g 205 Al a Al a Hi s G u l Ie Pr o G u Al a 110 Tyr Val Val Thr Asn 190 Al a Sy Asp bet Val Leu Phe Sy G u G n Val Asp Asp 175 Val Leu G u Thr Hi s G u Sy Asn Al a Al a SIe Val Al a 160 Pr o Val Thr G n <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(769) <223> RXN02688 <400> 171 gtgcggaaga cagcacgccc caaaccgacc aactagctaa gctacacaag gcggacgaat gggttcgcgc agcaagcgaa ggaaggaaac ttaactagcc atg gcc ggc cgg att 115 IVet Ala Gy Arg Ile 1 att ttg cta cga cac ggg cag act cac aac aac gtc aaa cac ctc ctg 163 Page 226 Ile Leu Leu Arg His Gy On Gene Sequence 24- 2- 06. txt Thr His Asn Asn Val Lys His 15 Leu Leu gac Asp gcc Al a gcc Al a ctt Leu at t SIle ttt Phe ct c Leu gag G u at c SIle 150 agc Ser gat Asp gt g Val tgg Tr p gat cgc Ar g gaa G u gt g Val acc Thr ct c Leu at g bet ggc G y 120 t ac Tyr gac Asp ggc G y aac Asn gag G u 200 gac Asp cca Pr o ggc Gy agc Ser acc Thr gtt Val ggc Gy ctt Leu gac Asp cac Hi s gt c Val 170 gcg Al a aac Asn gga G y cac Hi s t cc Ser ttt Phe 75 gt g Val gat Asp cac Hi s gt g Val gac Asp 155 at c SIle tt c Phe ggt Gy gct Al a gaa G u at c I e gaa gau gaa G u gaa G u ggg G y ct g Leu 140 ctt Leu cgc Ar g aac Asn aag Lys cca Pr o 220 gaa G u ct a Leu 45 gt g Val aaa Lys ggc Gy gaa G u gat Asp 125 aac Asn gac Asp at c SIle acc Thr aaa Lys 205 ct c Leu gcc Al a ttg Leu gct Al a at t SIle gcc Al a 110 cct Pr o cgc Ar g gac Asp gt g Val t ac Tyr 190 ttc Phe gac Asp t ac Tyr gcc Al a gac Asp 80 gaa G u at g bet gct Al a cag G n cgc Ar g 160 aca Thr ggc G y caa G n ggc G y ggt Gy caa G n cag G n aac Asn t ac Tyr ctt Leu 130 act Thr gtt Val gca Al a tgc Cys gat Asp 210 cgt Ar g gag G u acc Thr t cc Ser gt c Val t cc Ser 115 ccc Pr o ctt Leu gcc Al a act Thr cgc Ar g 195 gtt Val aaa Lys cgc Ar g gcc Al a ggt Gy ggc Gy 100 cgc Ar g ggc G y gat Asp gtt Val ggt G y 180 ttc Phe gt g Val caa G n ct c Leu gtg Val gcg Al a gac Asp gca Al a ggt G y cga Ar g gtc Val 165 gtg Val gtg Val cgc Ar g agc cca ct g Ser Pro Leu tgg cag gag Trp Gn u taat t gagac caaaggctcg <210> 172 <211> 223 <212> PRT <213> Corynebacter i um gl utam cum <400> 172 IVet Ala y Arg lie lie Leu Leu Arg His y n Thr His Asn Asn 1 5 10 Val Lys His Leu Leu Asp Thr Arg Pro Pro G y Ala G u Leu Thr Asp Page 227 Gene Sequence 24-2-06.txt Val y His G u Al a Thr Tyr Leu Ser G n vet SIle Asn Sy Pr o 145 Asp Hi s Asn Tr p Ar g G u Thr Ser Val Ser 115 Pr o Leu Al a Thr Ar g 195 Val G n Leu Val Al a Asp Al a Gy Ar g Val 165 Val Val Ar g Al a Al a Leu 70 l le Phe Leu G u SIle 150 Ser Asp Val Tr p Leu Hi s Al a Pr o G u Asn Thr 135 vet Hi s Pr o Leu Thr 215 Tyr Ser Asp Ar g 105 Tr p Lys Ser Al a Phe 185 Pr o Ser Ser Thr Val 90 Gy Leu Asp Hi s Val 170 Al a Asn Pr o Ser Phe Val Asp Hi s Val Asp 155 I le Phe Gy Leu Leu Val Lys Gy G u Asp 125 Asn Asp SIle Thr Lys 205 Tr p Leu Al a SIle Al a 110 Pr o Ar g Asp Val Tyr 190 Phe G n Ar g Ar g G n Hi s Al a Tyr Asp Al a 175 Leu Ser G u <210> 173 <211> 336 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(313) <223> RXN03087 <400> 173 gttgccgcca gccgttccag ggcgcttgag ctggtcagcg gaatacctgg aaaagagctg atattgatag ggtttaagtc cct ttt gct gga atc gtc cac tat ttt Pro Phe Ala y Ile Val His Tyr Phe gaa acc ggc atg caa ctg gga acg gta Su Thr y Net n Leu y Thr Val 30 cca atc atg gca ccg gga cct ggc atc Pro lie IVet Al a Pro G y Pro G y lle 45 gtc gat Val Asp 15 gaa acc G u Thr gt a gct Val Ala Page 228 acatcgcaat gatcaaccag atg aag atc tac gca IVet Lys Ile Tyr Ala 1 gaa ggc gat ccc gtg Su y Asp Pro Val atc aaa ctc gag gca Ile Lys Leu G u Ala aag gtt tct ttt gat Lys Val Ser Phe Asp Gene Sequence 24- 2- 06. txt gat ttc tcc gac gtc acc ggc ggc gat gaa ctc ctc gaa ttg gag gca 307 Asp Phe Ser Asp Val Thr Gy y Asp G u Leu Leu G u Leu G u Ala 60 aag aac taatgggtca aacccgcatc att 336 Lys Asn <210> 174 <211> 71 <212> PRT <213> Corynebacter i um gl utam cum <400> 174 Met Lys lie Tyr Ala Pro Phe Ala Gy Ile Val His Tyr Phe Val Asp 1 5 10 Su Gy Asp Pro Val G u Thr Gy Vet G n Leu y Thr Val G u Thr 25 Ile Lys Leu Gu Ala Pro Ile IVet Ala Pro Gy Pro Gy Ile Val Ala 40 Lys Val Ser Phe Asp Asp Phe Ser Asp Val Thr Gy Gy Asp G u Leu 55 Leu G u Leu G u Ala Lys Asn <210> 175 <211> 310 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(310) <223> RXN03186 <400> 175 ttcgtgcact tcggcgtgtc acaattaggt acgaccaaga atgggaccgg gaaaccggga cgtataaacg aaataaaaca ttccaacagg aggtgtggaa atg gcc gat caa gca 115 IVet Ala Asp G n Ala 1 aaa ctt ggt ggc aag ccc tcg gat gac tct aac ttc gcg atg atc cgc 163 Lys Leu G y G y Lys Pro Ser Asp Asp Ser Asn Phe Al a IVet Ile Arg 15 gat ggc gtg gca tct tat ttg aac gac tca gat ccg gag gag acc aac 211 Asp y Val Ala Ser Tyr Leu Asn Asp Ser Asp Pro G u G u Thr Asn 30 gag tgg atg gat tca ctc gac gga tta ctc cag gag tct tct cca gaa 259 G u Trp IVet Asp Ser Leu Asp y Leu Leu G n u Ser Ser Pro G u 45 cgt gct cgt tac ctc atg ctt cgt ttg ctt gag cgt gca tct gca aag 307 Arg Ala Arg Tyr Leu IVet Leu Arg Leu Leu G u Arg Ala Ser Ala Lys 60 cgc 310 Ar g Page 229 Gene Sequence 24-2-06.txt <210> 176 <211> <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 176 IVet Al a Asp G n Al a Lys Leu G y G y Lys Pro Ser Asp Asp Ser Asn 1 5 10 Phe Al a IVet Ile Arg Asp G y Val Al a Ser Tyr Leu Asn Asp Ser Asp 25 Pro G u G u Thr Asn G u Trp IVet Asp Ser Leu Asp y Leu Leu G n 40 Su Ser Ser Pro G u Arg Ala Arg Tyr Leu IVet Leu Arg Leu Leu G u 55 Arg Ala Ser Ala Lys Arg <210> 177 <211> 302 <212> DNA <213> Cor ynebact er i um gl ut ari cum <220> <221> CDS <222> <223> RXN03187 <400> 177 gtt gca gtg tct gac ttc tcc act gat ctg cca aac cag atc cgt gaa 48 Val Ala Val Ser Asp Phe Ser Thr Asp Leu Pro Asn n lie Arg G u 1 5 10 tgg gtc cca ggc gac tac acc gtt ctc ggt gca gat ggc ttc ggt ttc 96 Trp Val Pro y Asp Tyr Thr Val Leu y Ala Asp y Phe y Phe 25 tct gat acc cgc cca gct gct cgt cgc ttc ttc aac atc gac gct gag 144 Ser Asp Thr Arg Pro Al a Ala Arg Arg Phe Phe Asn Ile Asp Ala G u 40 t cc att gtt gtt gca gtg ctg aac tcc ctg gca cgc gaa ggc aag atc 192 Ser lie Val Val Ala Val Leu Asn Ser Leu Ala Arg G u y Lys Ile 55 gac gtc tcc gtt gct gct cag gct gct gag aag ttc aag ttg gat gat 240 Asp Val Ser Val Ala Al a G n Ala Ala G u Lys Phe Lys Leu Asp Asp 70 75 cct acg agt gtt tcc gta gat cca aac gct cct gag gaa taaatcacct 289 Pro Thr Ser Val Ser Val Asp Pro Asn Ala Pro G u G u caagggacag ata 302 <210> 178 <211> 93 <212> PRT <213> Cor ynebact er i um gl ut ai cum <400> 178 Val Ala Val Ser Asp Phe Ser Thr Asp Leu Pro Asn n lie Arg G u Page 230 1 Trp Val Ser Asp Ser Ile Asp Val Pro Thr Pr o Thr Val Ser Ser 5 Asp Pr o Al a Al a Ser Tyr Thr Al a Al a Val Leu 55 Al a G n 70 Val Asp Gene Sequence 24-2-06.txt 10 Val Leu Gy Ala Asp y Phe y Phe Arg Arg Phe Phe Asn I e Asp Ala G u 40 Asn Ser Leu Ala Arg Gu Gy Lys Ile Ala Ala G u Lys Phe Lys Leu Asp Asp 75 Pro Asn Al a Pro G u G u <210> 179 <211> 1953 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1930) <223> RXN02591 <400> 179 atgtgtccgt tgtctcacct aaagttttaa ctagttctgt ggcgagaact ctgtcgaatg acacaaaatc tggagaagt a atctgaaagc tacgctaggg atg act act gct gca 11! Met Thr Thr Ala Ala at c SIle aac Asn ttc Phe gtt Val agc Ser cgc Ar g aac Asn gct Al a ggc G y at c SIle gat Asp gcc Al a ct a Leu ttc Phe gct Al a t cc Ser 120 cag G n gac Asp t cc Ser acc Thr cgt Ar g tgc Cys cca Pr o aag Lys ggc G y gcc Al a cag G n ct c Leu t cc Ser 75 t cc Ser cag G n ggg Gy gag G u gt c Val gct Al a at c SIle aac Asn gag G u gca Al a cgc Ar g gcg Al a gag G u gag G u 45 aag Lys cca Pr o aag Lys at g Net acc Thr 125 ccg Pr o ct c Leu tgg Tr p ct c Leu tct Ser gaa G u aag Lys 110 at g bet aag Lys cag G n cgc Ar g gag G u gtt Val gat Asp gaa G u gt c Val 1 aat Asn cct Pr o at g bet gaa G u gcg Al a gct Al a at g Net gt g Val aag Lys gag G u gcg Al a aag Lys cgc Ar g ggc G y t cc Ser cct Pr o 130 gaa G u gct Al a gag G u cgt Ar g gtt Val cca Pr o aag Lys 115 ttc Phe ctg Leu gtt Val gat Asp ccg Pr o gag G u acc Thr 100 cat Hi s tgc Cys ggt cca atc agc gat ccg gac cct aag ctt ggt gtg cag ctc act gac Page 231 Gy Pro lie Ser Asp Pro 135 t cc Ser 150 gaa G u cac Hi s cct Pr o at t SIle aag Lys 230 tgg Tr p aag Lys aac Asn gtt Val gca Al a 310 t ac Tyr ct g Leu ggc Gy gac Asp t ac Tyr 390 t ac Tyr ct g Leu gtt Val aac Asn 200 t cc Ser t ac Tyr gct Al a t ac Tyr gcc Al a 280 gac Asp aac Asn t cc Ser acc Thr gac Asp 360 acc Thr gt a Val gt c Val aag Lys 170 gct Al a acc Thr ggt Gy ct g Leu cac Hi s 250 at c SIle at c l Ie at c SIle gaa G u cca Pr o 330 gt g Val gac Asp gag G u at c SIle at g bet 155 at c SIle cct Pr o aag Lys t cc Ser cgt Ar g 235 at g bet gca Al a act Thr gct Al a aat Asn 315 at c SIle gca Al a gcc Al a t cc Ser gac Asp 395 Asp 140 t cc Ser ggc Gy ttg Leu t ac Tyr ggc Gy 220 at c SIle ct c Leu gca Al a cca Pr o tgg Tr p 300 ggt G y gcg Al a ct c Leu cca Pr o gac Asp 380 cag G n Gene Sequence 24-2-06.txt Pro Lys Leu y Val n Leu Thr Asp at g et gcg Al a gag G u at c SIle 205 t ac Tyr gca Al a at c SIle gca Al a acc Thr 285 ct g Leu ttc Phe at g Net acc Thr gct Al a 365 gaa G u t cc Ser cgc Ar g aac Asn cca Pr o 190 acc Thr ggc Gy tct Ser ct g Leu ttc Phe 270 at c SIle aag Lys ttc Phe aag Lys gac Asp 350 cac Hi s aac Asn cca Pr o at c SIle ggc Gy 175 ggc Gy cag G n gga Gy gt c Val aag Lys 255 cca Pr o cca Pr o ct g Leu ggt G y acc Thr 335 gac Asp ct c Leu gct Al a gca Al a at g bet 160 agc Ser cag G n ttc Phe aac Asn at g Net 240 ct g Leu tct Ser ggc G y cgc Ar g gtt Val 320 at g Net ggc Gy at t gct Al a gca Al a 400 145 acc Thr ttc Phe gaa G u cca Pr o gca Al a 225 gct Al a at c SIle gct Al a tgg Tr p gag G u 305 gct Al a gaa G u gac Asp gac Asp cac Hi s 385 gca Al a cgc Ar g gt c Val gac Asp gag G u 210 at c l Ie cgc Ar g aac Asn tgt Cys acc Thr 290 gac Asp cca Pr o cca Pr o at c SIle tgg Tr p 370 cct Pr o cct Pr o at g let agg Ar g gtt Val 195 acc Thr ct g Leu gaa G u cca Pr o ggc G y 275 gct Al a ggc Sy ggc G y ggc Gy tgg Tr p 355 at g Net aac Asn gag G u ggt G y tgc Cys 180 gca Al a aag Lys gca Al a gaa G u gag G u 260 aag Lys cag G n ct c Leu acc Thr aac Asn 340 tgg Tr p ggc Gy t cc Ser ttc Phe 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 gac tgg gaa ggc gtc aag atc gac gca atc ctc ttc ggt gga cgt cgc Page 232 Asp Trp Gu Gy Val Lys Ile 410 Gene Sequence 24-2-06.txt Asp Ala lle Leu Phe Gy y 415 Arg Arg 420 gca gac acc Al a Asp Thr acc atg gtt Thr [et Val 440 gaa gca aag G u Al a Lys 455 ttc att ggc Phe I e G y 470 ggt aac aag G y Asn Lys ttc cgc cgt Phe Ar g Ar g aac t ct cgc Asn Ser Arg 520 ggc gca gac G y Al a Asp 535 gac ctc gac Asp Leu Asp 550 acc gct cct Thr Ala Pro tac ct c act Tyr Leu Thr ttc gat gct Phe Asp Al a 600 cca Pr o gca Al a ggc G y aac Asn ggc G y 490 gaa G u ct g Leu acc Thr ct c Leu gag G u 570 ct c Leu ct g Leu ct g Leu aca Thr gct Al a 475 gac Asp gat Asp aag Lys gtt Val gac Asp 555 cag Sn gga G y gtt Val ct c Leu ct c Leu 460 ggt G y aag Lys gga G y tgg Tr p gtt Val 540 acc Thr tgg Tr p cca Pr o acc Thr gca Al a 445 cgc Ar g gaa G u at g Net cgc Ar g gt c Val 525 gga G y cca Pr o gca Al a cgt Ar g at t SIle 605 cag G n 430 t cc Ser cac Hi s t ac Tyr cca Pr o ttc Phe 510 at c SIle cac Hi s at t SIle aac Asn gtt Val 590 t ac Tyr cag G n cca Pr o cag G n 480 at c SIle tgg Tr p cgc Ar g gct Al a gat Asp 560 gtt Val gca Al a tgg Tr p gca Al a 450 gca Al a tgg Tr p ct g Leu ggc G y gaa G u 530 gcc Al a aag Lys gac Asp gtt Val gct Al a 610 gag G u 435 gct Al a at g bet at t SIle gt c Val ttc Phe 515 ggc G y gaa G u gaa G u aac Asn cac Hi s 595 cac Hi s t cc Ser ct c Leu gac Asp aac Asn 500 ggc G y cac Hi s gac Asp gca Al a gcc Al a 580 agc Ser ggc G y gca Al a cca Pr o at g bet 485 tgg Tr p gac Asp gtt Val ct c Leu ct g Leu 565 gag G u cag G n 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1940 1953 aag gcc cgc Lys Al a Ar g t ca gca gct cac Ser Ala Ala His t aaagt t cac gct t aagaac tgc <210> 180 <211> 610 <212> PRT <213> Corynebacter i um gl utam cum <400> 180 IVet Thr Thr Ala Ala lIe Arg y Leu On Gy u Ala Pro Thr Lys 1 5 10 Asn Lys G u Leu Leu Asn Trp lie Ala Asp Ala Val G u Leu Phe G n 25 Pro u Ala Val Val Phe Val Asp G y Ser G n Ala G u Trp Asp Arg 40 Page 233 ene Sequence 24- 2- 06. txt I'bt Ala Ou Asp Leu Val Ou Ala G1y Thr Leu I Ie Lys Leu Asn O31u (31u Al a Al a vb t ValI ValI 145 Thr Ph e (31u Pr o Al a 225 Al a I Ie Al a Tr p (31u 305 Al a (31u As p As p Hi s 385 Ar g ValI Pr o Ly s 115 Ph e Le u vb t Ar g ValI 1 95 Thr Le u (31u Pr o G31y 275 Al a G31y G31y G31y Tr p 355 vb t As n As n Ser As n Tyr vb t As p I Ie 1 65 Le u Tr p (31u Ly s G31y 245 G31y Thr ValI Tyr As n 325 Thr (31u As n Ar g Ser 70 Ar g As n Al a G31y Ser 150 (31u Hi s Pr a I Ie Ly s 230 Tr p Ly s As n ValI Al a 310 Tyr Le u G31y As p Tyr 390 Tyr Thr Tr p G31y Pr a 135 (31u Al a Ser cys Tr p 215 cys vb t Al a Le u G31y 295 ValI Al a Ph e vb t Tr p 375 Cys Le u Ph e Al a Ser 120 li1e Tyr Le u ValI As n 200 Ser Tyr Al a Tyr Al a 280 As p As n Ser Thr As p 360 Thr ValI Al a I Ie Pr a 1 vb t Ser ValI As p G y 1 As p Tyr Al a 0 u Hi s 265 vb t As p Pr a As n As n 345 0Gy Pr a Al a Ar g cys 90 Pr a Ly s As p ValI Ly s 1 70 Al a Thr G31y Le u Hi s 250 le le le Ou Pr a 330 ValI As p (31u I Ie Ser Ser O31n G31y Pr a vb t 1 li1e Pr a Ly s Ser Ar g 235 vb t Al a Thr Al a As n 315 li1e Al a Al a Ser As p 395 As n (31u Al a Ar g As p 140 Ser G31y Le u Tyr G31y 220 li1e Le u Al a Pr a Tr p 300 G31y Al a Le u Pr a As p 380 O31n Pr a Ly s vb t Thr 125 Pr a vb t Al a G u I Ie 205 Tyr Al a I Ie Al a Thr 285 Le u Ph e vb t Thr Al a 365 0Ou Ser As p O31u As p Tyr Le u G31y 1 G y O31n G31y ValI Ly s 255 Pr a Pr a Le u G31y Thr 335 As p Le u Al a Al a Al a Pro 0 u Phe Asn Asp Trp 0 u 0 y Val Lys I e Asp Ala Il e Leu Page 234 Gene Sequence 24-2-06.txt Phe Asp Thr vet 465 Asn Phe Pr o SIle Lys 545 Val G u Gy G u 435 Al a vet SIle Val Phe 515 Gy G u G u Asn 405 Ar g Gy Al a Pr o vet 485 Tr p Asp Val Leu Leu 565 G u 410 Pro Leu Val Al a Thr G u Phe 470 Gy Phe Asn Gy Asp 550 Thr Ty r Asp Nbet Al a 455 I le Asn Ar g Ser Al a 535 Leu Al a Leu Val 425 Gy Val Tyr Gy Gy 505 Val G u Gy Al a Phe 585 Al a Gy Asn Gy 490 G u Leu Thr Leu G u 570 Leu Leu Thr Al a 475 Asp Asp Lys Val Asp 555 G n Gy Thr G n 430 Al a Ser 445 Arg Hi s G u Tyr Net Pro Arg Phe 510 Val lile 525 G y Hi s Pro Ile Al a Asn Arg Val 590 415 Thr Tyr G y G n Asp Pro Leu G n 480 Ser Ile 495 Leu Trp Asp Arg Thr Al a Su Asp 560 Asp Val 575 Pro Al a G u Val Hi s 595 Ser G n Phe Asp Al a 600 Leu Lys Ala Arg SIle 605 Ser Al a Al a Hi s Al a 610 <210> 181 <211> 1305 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1282) <223> RXS01260 <400> 181 ct aaacgt gg gctgcattcc ttccaaagtc t ct gat caaa aacgct gaag ttgcccatac ctttacccat gagaagaaga ccttcggcat caatggcgaa gtg acc ttc aac tat Val Thr Phe Asn Tyr 1 gag gat gct cac aag cgt tcc cgt ggc gtt tcc gac aag atc gtt gga G u Asp Ala His Lys Arg Ser Arg G y Val Ser Asp Lys IIe Val G y 15 ggc gtt cat tac ttg atg aag aag aac aag atc atc gaa att cat ggt Sy Val His Tyr Leu IVet Lys Lys Asn Lys lie lie G u lie His y 30 ctt gga aac ttc aag gat gct aag act ctt gag gtc acc gac ggt aag Page 235 Gene Sequence 24-2-06.txt Leu y Asn Phe Lys Asp Ala Lys Thr Leu G u Val Thr Asp y Lys 45 gat gct ggc aag acc atc acc ttt gat gac tgc atc atc gca acc ggt 307 Asp Ala G y Lys Thr Ile Thr Phe Asp Asp Cys lie lie Ala Thr y 60 tcg gta gtc aac acc ctc cgt ggc gtt gac ttc tca gag aac gtt gtg 355 Ser Val Val Asn Thr Leu Arg y Val Asp Phe Ser G u Asn Val Val 75 80 tct ttt gaa gag cag att ctt aac cct gtt gcg cca aag aag atg gtc 403 Ser Phe G u G u n Ile Leu Asn Pro Val Ala Pro Lys Lys IVbt Val 95 100 att gtt ggt gca ggc gca att gga atg gaa ttc gcc tac gtt ctt ggt 451 Ile Val y Al a y Ala Ile y IVbt u Phe Ala Tyr Val Leu y 105 110 115 aac tac ggt gta gat gta acc gtc atc gag ttc atg gat cgt gtg ctt 499 Asn Tyr y Val Asp Val Thr Val Ile G u Phe IVbt Asp Arg Val Leu 120 125 130 cca aat gaa gat gct gaa gtc tcc aag gtt att gca aag gcc tac aag 547 Pro Asn G u Asp Ala G u Val Ser Lys Val Ile Ala Lys Ala Tyr Lys 135 140 145 aag atg ggc gtt aag ctt ctt cct ggc cat gca acc act gct gtt cgg 595 Lys IVt G y Val Lys Leu Leu Pro G y His Ala Thr Thr Ala Val Arg 150 155 160 165 gac aac ggt gac ttt gtc gag gtt gat tac cag aag aag ggc tct gac 643 Asp Asn y Asp Phe Val G u Val Asp Tyr G n Lys Lys Gy Ser Asp 170 175 180 aag aca gag act ctt act gtt gat cga gtc atg gtt tcc gtt ggt ttc 691 Lys Thr G u Thr Leu Thr Val Asp Arg Val lVbt Val Ser Val G y Phe 185 190 195 cgt cca cgc gtt gag gga ttt ggt ctt gaa aac act ggc gtt aag ctc 739 Arg Pro Arg Val G u G y Phe Gy Leu G u Asn Thr Gy Val Lys Leu 200 205 210 acc gag cgt ggc gca at c gag atc gat gat tac atg cgt acc aac gtc 787 Thr u Arg G y Ala lie u lie Asp Asp Tyr IVbt Arg Thr Asn Val 215 220 225 gat ggc att tac gcc atc ggt gac gtg acc gcc aag ctt cag ctt gct 835 Asp Gy Ile Tyr Ala lie y Asp Val Thr Ala Lys Leu n Leu Ala 230 235 240 245 cac gtc gca gaa gca cag ggc att gtt gcc gca gag act att gct ggt 883 His Val Ala G u Al a Gn G y IIe Val Ala Al a u Thr Ile Ala G y 250 255 260 gca gaa act cag act ctt ggt gat tac atg atg atg cca cgt gca acc 931 Al a G u Thr G n Thr Leu G y Asp Tyr IVbt IVbt IVbt Pro Arg Al a Thr 265 270 275 ttc tgc aac cca cag gtt tct tcc ttt ggt tac acc gaa gag cag gcc 979 Phe Cys Asn Pro G n Val Ser Ser Phe y Tyr Thr G u G u G n Ala 280 285 290 aag gag aag tgg cca gat cgt gag atc aag gtt gct tcc ttc cca ttc 1027 Lys G u Lys Trp Pro Asp Arg G u lie Lys Val Ala Ser Phe Pro Phe 295 300 305 tct gca aac ggt aaa gca gtt ggc ctg gca gaa act gat ggt ttc gca 1075 Page 236 ene Sequence 24- 2- 06. txt Ser Al a Asn G31y Lys Ala Val Gy Leu Ala (31u Th r As p G3y 310 315 320 aag at c gt t gct gat gca gaa t tc ggt gag ct g ct c ggt gca Lys Ilie Val Al a Asp Al a G31u Phe G31y (31u Leu Leu G31y Al a 330 335 gt t gga gca aat gca tca gag ct cat caat gaat t ggt gct t Val G31y Al a Asn Al a Ser G31u Leu Ilie Asn G31u Leu Val Leu 345 350 355 aac t gg gat ct c acc act gaa gag at c t ct cgt agc gt c cat Asn Tr p Asp Leu Thr Thr G31u 31u Ilie Ser Ar g Ser Val Hi s 360 365 370 cca acg ct a t ct gag gca gt t aag gaa gct gca cac ggt at c Pr o Thr Leu Ser G31u Al a Val Lys G31u Al a Al a Hi s G31y Ilie 375 380 385 cac at g at c aac t tc t agaat ccac ct cgt tggcc ct g His 'b t Ilie Asn Phe 390 <210> 182 <211> 394 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn Ph e c ac Hi s 340 gct Al a at t I Ie t ct Ser 1123 1171 1219 1267 1305 ValI As p I Ie ValI I Ie Ser Pr o Al a vb t Al a 145 Thr ValI O> 182 Thr Phe Asn Tyr Lys I I e Val (3 u I I e Hi s Thr As p G3 y 1 1 e Al a Thr (3 u As n Val Lys Lys I'b t 1 00 Ty r Val Leu 115 As p Ar g Val 130 Lys Al a Ty r Thr Al a Val Lys G3 y Ser 1 80 Ser Val (3y 1 95 a31y a31y Lys5 a31y ValI ValI a31y Le u Lys5 Ar g 1 65 As p Ph e 31u As p a3 y Val Leu a3 y As p Al a 55 Ser Val 70 Ser Phe I e Val Asn Tyr Pr o As n 135 Lys I'b t 150 As p As n Lys Thr Ar g Pr o Al a Hi s As n 40 a31y ValI a31u a31y a31y 120 a31u a31y a31y a31u Ar g 200 Hi s Tyr Ph e Lys5 As n a31u Al a 1 ValI As p ValI As p Thr 1 ValI Ar g vb t As p I Ie Le u 75 li1e Al a ValI a31u Le u 1 55 ValI Thr Ser Lys5 Al a Thr Ar g Le u I Ie Th r ValI 140 Le u a31u ValI Ar g Ly s Ly s Ph e a31y As n a31y ValI 125 Ser Pr o ValI As p (31y 205 ValI Ly s Le u As p As p ValI a31u a31u ValI Hi s Tyr 1 ValI Ser I Ie a31u cys Ph e Al a Ph e Ph e I Ie Al a 1 a31n vb t 03 u 03 y Phe Leu 03 u Asn Page 237 Gene Sequence 24-2-06.txt Thr G y 210 Ibet Arg 225 Lys Leu G u Thr IVet Pro Thr G u 290 Al a Ser 305 Thr Asp Leu G y Leu Val Ser Val 370 Hi s G y 385 Val Lys Leu Thr u Arg y Ala lie Gu lie Asp Asp Tyr Thr G n SIle Ar g 275 G u Phe Sy Al a Leu 355 Hi s SIle Val Al a 245 Sy Thr Al a Phe Al a 325 Leu G n Hi s Sy 215 Sy Val G u Cys G u 295 Al a l Ie Gy Tr p Thr 375 bet SIle Al a Thr Asn 280 Lys Asn Val Al a Asp 360 Leu I le Tyr G u G n 265 Pr o Tr p Sy Al a Asn 345 Leu Ser Asn Al a Al a 250 Thr G n Pr o Lys Asp 330 Al a Thr G u Phe l Ie 235 G n Leu Val Asp Al a 315 Al a Ser Thr Al a Asp SIle Asp Ser 285 G u Sy Phe Leu G u 365 Lys Thr Al a 255 vet Sy Lys Al a G u 335 Asn Ser Al a <210> 183 <211> 294 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(271) <223> RXS01261 <400> 183 gtgggtgttt ttcattttct tccactctaa aattaagtat atgcacgaca atgacccact aaacacgtat ccttgaatgc gac Asp cgt Ar g tgg Tr p gat gt a Val gcg Al a ggt G y aaa ctc gga gcc ggc ccc ggt ggc Leu Gy Ala Gy Pro y y 15 ctt ggc aag aag gtt gct gta Leu G y Lys Lys Val Ala Val tgc cta aac gtg ggc tgc att Cys Leu Asn Val y Cys Ile 45 t gaagt t gcc cataccttta ccc Page 23E ggaaaaccaa ccgcacccgg gtg act gaa cat tat Val Thr G u His Tyr 1 tat gtc tcc gcc atc Tyr Val Ser Ala lle att gag aag cag tac Ile G u Lys G n Tyr cct tcc aaa gtc tct Pro Ser Lys Val Ser Gene Sequence 24-2-06.txt Asp G n Lys Arg <210> 184 <211> 57 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 184 Val Thr G u His Tyr Asp Val Val Val Leu G y Ala G y Pro G y G y 1 5 10 Tyr Val Ser Ala I e Arg Ala Ala G n Leu y Lys Lys Val Ala Val 25 Ile u Lys n Tyr Trp y y Val Cys Leu Asn Val y Cys Ile 40 Pro Ser Lys Val Ser Asp G n Lys Arg <210> 185 <211> 1650 <212> DNA <213> Cor ynebact er i um gl ut ai cum <220> <221> CDS <222> (101)..(1627) <223> RXA02640 <400> 185 accaacgacg acgccggtgt agcagatgta ttggagtggt ggttctaata ggtggtgtta aaacactgct tagtggccca atacgtgcaa aaataaggcc aga at c t ca Arg Ile Ser gcc Al a tgc Cys gag G u gaa G u t cc Ser cag G n t ac Tyr gcg Al a ttc Phe cgc Ar g at t SIle gac Asp gaa G u gca Al a gtt Val gat Asp at c SIle gac Asp acc Thr acc Thr gt g Val gca Al a gcc Al a tt c Phe aac Asn cca Pr o 75 gt g Val tgg Tr p gcg Al a caa G n cca Pr o at t SIle cac Hi s gt g Val caa G n at t l Ie gga Sy caa G n 45 cga Ar g gag G u tgg Tr p gac Asp gac Asp aaa Lys cag G n tct Ser gtt Val gac Asp acc Thr 110 caa G n 15 gt g Val ggc G y gt c Val gca Al a aag Lys 95 cgc Ar g ggc Sy gt g Val tgg Tr p gt c Val tcg Ser cac Hi s acc Thr act Thr tct Ser gag G u cag G n gga G y ggc G y gac Asp t cc Ser gct Al a cac Hi s gcg Al a gt c Val gaa G u at t SIle 115 act Thr t cc Ser gat Asp at g bet acc Thr cct Pr o 100 tgc Cys Page 239 Gene Sequence 24-2-06.txt gag atc gcg ggc gaa gaa ggc cag gaa G u Ile Al a G y G u G u Gy G n G u aag tgg ct t Lys Trp Leu gac cgc acc ggc Asp Arg Thr y 130 ct g Leu gac Asp 150 ttt Phe cgc Ar g gca Al a gaa Gu cgt Ar g 230 gcc Al a ttt Phe acc Thr gag G u ccg Pr o 310 cag G n gaa G u cca Pr o ggc Sy 120 at c SIle gtt Val acc Thr gac Asp cgc Ar g 200 tgc Cys t cc Ser gt c Val cag G n ct c Leu 280 ggc Sy t ac Tyr ct g Leu ct c Leu ttc Phe 360 at t SIle t cc Ser gga Sy gat Asp 170 ggt Sy ct a Leu gcc Al a gga Sy at t SIle 250 gga Sy ct g Leu ct g Leu ct g Leu gac Asp 330 cga Ar g gga Sy ggc Gy tac Tyr gct Al a 155 acc Thr gat Asp tt g Leu ct a Leu gaa G u 235 act Thr tt c Phe ct g Leu t cc Ser gaa G u 315 aac Asn gaa G u ct g Leu ct c Leu cca Pr o 140 cgc Ar g tgg Tr p gat Asp at g Met gac Asp 220 ttc Phe ggc Sy cac Hi s at g Met acc Thr 300 ggt G y ct a Leu gt c Val ttc Phe acc Thr 380 125 tcg Ser gaa G u gt g Val gcc Al a gat Asp 205 at t SIle cgc Ar g gt g Val gaa G u aac Asn 285 at c SIle t cc Ser cag G n gaa G u gca Al a 365 cgt Ar g ggg Gy cgc Ar g ct g Leu at c l Ie 190 ct c Leu ccg Pr o t cc Ser ct c Leu ggt Sy 270 acc Thr gcc Al a gt a Val ct a Leu gac Asp 350 cca Pr o ttt Phe aaa Lys gaa G u 160 aac Asn gt c Val acg Thr t cc Ser cgc Ar g 240 gac Asp gct Al a acc Thr caa G n at g let 320 ccc Pr o ggt G y tgg Tr p aac Asn at c SIle 145 aag Lys ct g Leu acc Thr caa G n at g bet 225 cac Hi s cag G n aaa Lys tcg Ser cgg Ar g 305 ggc G y aac Asn ggc G y cgc Ar g cgc Ar g 385 aag Lys ggc G y acc Thr gat Asp cag G n 210 ct c Leu cgc Ar g caa G n aat Asn ttg Leu 290 gaa G u ggt G y gca Al a gtt Val ccc Pr o 370 aaa Lys tgg Tr p gac Asp ggc G y gt c Val 195 tgg Tr p cct Pr o gga Sy gcg Al a acc Thr 275 aag Lys gga Sy t cc Ser cca Pr o cat Hi s 355 gat Asp cac Hi s at t SIe ctt Leu ggt G y 180 acc Thr gat Asp gag Gu acc Thr gcc Al a 260 t ac Tyr at t SIe t cc Ser ttg Leu gcg Al a 340 gtt Val gct Al a at c SIe 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 Page 240 cgc Ar g 390 gcc Al a ggt Sy ct c Leu ggc Sy gac Asp 470 at g Met gt g Val gt c Val gcc Al a at g Met at c SIle 440 gca Al a at c SIle gaa Gu cat Hi s gaa G u gac Asp 410 gaa G u gt c Val gct Al a aaa Lys gag Gu 490 t at Tyr gcc Al a 395 gca Al a aat Asn caa G n gca Al a ctt Leu 475 cgc Ar g gac Asp aac Asn ggc Gy gac Asp cgt Ar g ggt Gy 460 at t SIle gaa Gu cag G n Gene Sequence 24-2-06.txt gcc ttc caa acc cgc gaa gtt gtg Ala Phe G n Thr Arg G u Val Val 400 aaa gcc ctc gaa tcc ctc cgc gtc Lys Ala Leu u Ser Leu Arg Val 415 420 ctc ctc atg caa atg caa gcc gac Leu Leu IVet G n Iet G n Al a Asp 430 435 ctc gag gac gta gaa acc acc gcc Leu G u Asp Val G u Thr Thr Ala 445 450 ctc ggc tct gga ttc ttc aaa aca Leu G y Ser G y Phe Phe Lys Thr 465 gca gtg aag aaa gtc tgg aac cct Ala Val Lys Lys Val Trp Asn Pro 480 cgt cgc tat gcc gaa tgg aat agg Arg Arg Tyr Ala G u Trp Asn Arg 495 500 gcc tagctgattt gggtcggcct tta Al a gac Asp 405 gac Asp ttc Phe gt c Val act Thr gac Asp 485 gca Al a 1315 1363 1411 1459 1507 1555 1603 1650 <210> 186 <211> 509 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 186 NIet Arg lie Ser Lys Ala Asn Ala T 1 5 Thr Thr Ser Thr Arg Cys lie Phe I Ser Ser Ala Ser Lys u His Arg C 40 Val u His Asp Pro Gu Ou Ile T Ser n Ala IVet Val Ser lie Asp I 70 Leu y Val Thr Asn G n Arg G u T Thr Gy G u Pro Val Tyr Asn Ala I 100 1 Ser Asp I e Cys Leu G u I e Ala C 115 120 Leu Asp Arg Thr y Leu Leu Ile A 130 135 Ile Lys Trp lie Leu Asp Asn Val C 145 150 yr Val Ala Ala l e Asp On y le Sn rp le hr le ay sn Su 10 Asp Al a Ile Phe I Asp Asn I Thr Pro I Thr Val 90 Val Trp G u G u i Ser Tyr I G y Ala 155 Page 241 Sy G n Ar g G u Tr p Asp G n 125 Ser G u Val Sy Val Al a Lys Ar g Lys Pr o Al a Gene Sequence 24-2-06.txt Lys G y Asp Leu Leu Phe G y Leu Thr G n bet 225 Hi s G n Lys Ser Ar g 305 Gy Asn Gy Ar g Ar g 385 Ar g Ser bet G u Phe 465 Val G u Gy Val 195 Tr p Pr o Gy Al a Thr 275 Lys Gy Ser Pr o Hi s 355 Asp Hi s Val Ar g Al a 435 Thr Lys Asn Asn 165 Val Asn Pr o SIle Leu 245 Leu Gy Ser Al a Val 325 I le Val Ar g Al a Asp 405 Asp Phe Val Thr Asp 485 Al a Ar g Al a G u Ar g 230 Al a Phe Thr G u Pr o 310 G n G u Pr o Gy Ar g 390 Al a Gy Leu Gy Asp 470 Vet Val Gy Ser Leu 215 Pr o Asp Gy Gy Hi s 295 Val Tr p Asn Al a Val 375 Al a vet Al a Gy Val 455 G u Ser G u Thr IVet Asp 170 Asp Asp G y 185 Arg Thr Leu 200 Cys G u Al a Ser Val G y Val Pro Ile 250 Gn y y 265 Leu Phe Leu 280 G y Leu Leu Tyr Ala Leu Leu Arg Asp 330 Leu Al a Ar g 345 Phe Thr G y 360 I e Thr G y Val Leu G u Ala Lys Asp 410 [et Val G u 425 Ile Asp Val 440 Al a Phe Al a Ile G u Lys G u G u G u 490 Hi s Ser Tyr 505 Thr Asp Leu Leu G u 235 Thr Phe Leu Ser G u 315 Asn G u Leu Leu Al a 395 Al a Asn G n Al a Leu 475 Ar g Asp Trp Val Leu Trp Asn 175 Al a Asp 205 I le Ar g Val G u Asn 285 I le Ser G n G u Al a 365 Ar g Al a Lys Leu Leu 445 Leu Al a Ar g Al a l Ie 190 Leu Pr o Ser Leu Gy 270 Thr Al a Val Leu Asp 350 Pr o Phe Phe Al a Leu 430 G u Gy Val Ar g Hi s Ar g vet Val Gy 255 Al a Gy Tyr Ser SIle 335 Asn Ar g Al a G n Leu 415 et Asp Ser Lys Tyr 495 Val Thr Ser Ar g 240 Asp Al a Thr G n vet 320 Pr o Sy Tr p Asn Thr 400 G u G n Val Sy Lys 480 Al a Page 242 Gene Sequence 24-2-06.txt <210> 187 <211> 1119 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1096) <223> RXN01025 <400> 187 gggcagcagc ggcaggtttc caggaggttt ccatgcgggt ctgagacggt taaatatcgt tttcgaaagg tgggtttcgc ggcttgggac atgggctaac gt g Val gat Asp agc Ser acg Thr gag G u ggc Gy gt g Val gaa G u ttg Leu gct Al a 150 gct Al a gt g Val tgt Cys ggt Sy ggc G y at c I e ccg Pr o gca Al a ttg Leu ttg Leu at c SIle 120 ggg Sy gt g Val gt g Val act Thr at t SIle 200 ggt Sy gct Al a gac Asp tcg Ser at t l Ie gag G u aaa Lys gag Su aac Asn gct Al a gcg Al a 170 at c SIle cat Hi s tgg Tr p acg Thr cat Hi s cag G n gt g Val aaa Lys at t SIle acg Thr gct Al a 140 cct Pr o t at Tyr ggt G y t ac Tyr gga G y ttg Leu gaa G u 45 gt c Val ttg Leu gag G u gaa G u gaa G u 125 cgt Ar g gat Asp ttc Phe gcg Al a ggc Gy 205 acc Thr tgg Tr p aac Asn aca Thr gcg Al a acg Thr aag Lys 110 gcg Al a gag G u gaa G u cgc Ar g tgt Cys 190 ct g Leu acg Thr 15 gcg Al a cgt Ar g t ca Ser at t l Ie at c I e 95 ggc Gy gat Asp at c SIle aac Asn ccg Pr o 175 aag Lys ggt Gy ttg Leu agg Ar g gat Asp tcg Ser cct Pr o ccg Pr o acg Thr cct Pr o gcg Al a cga Ar g 160 t ac Tyr aac Asn gag G u gt g Val 1 gcc Al a cgg Ar g t ac Tyr gca Al a tcg Ser cag G n cac Hi s t ca Ser gag G u 145 gcg Al a acc Thr gt c Val aac Asn gtt tct gta Val Ser Val aag Lys gaa G u ctt Leu acg Thr cag G n gat Asp ct g Leu cgc Ar g 130 ggg G y aaa Lys aac Asn at c SIle acc Thr 210 gt c Val gag G u ccg Pr o gag G u gcg Al a gcg Al a cgg Ar g 115 at c SIle cag G n ctt Leu act Thr gcg Al a 195 aat Asn ttc Phe ttg Leu ggg G y gct Al a ttg Leu acc Thr 100 at g let gcg Al a cct Pr o gtg Val gat Asp 180 ctg Leu gca Al a Page 243 ttg Leu ggt Sy 230 gt g Val cgt Ar g ggt Sy gcc Al a gt g Val 310 ggc Gy act Thr gat Asp acg Thr ggt Sy gtt Val 280 aag Lys cac Hi s tct Ser ggc Gy aag Lys t ca Ser 250 ggt Sy gag Su ggt Sy gat Asp aag Lys 330 ctt Leu act Thr 235 t ca Ser gaa G u ggt G y gt g Val at g Met 315 gct Al a gca Al a 220 ttt Phe ccg Pr o t cc Ser gtt Val gag G u 300 aaa Lys gag G u Gene Sequence 24- 2- 06. txt gag atc gca cgc ctc ggt gcc Su lie Ala Arg Leu y Al a 225 tct ggc ctt gcg gga atg ggc Ser y Leu Ala G y IVt G y 240 ctg tcg cgt aac cgc agc ttc Leu Ser Arg Asn Arg Ser Phe 255 cta gag aag gct cgc gag gca Leu G u Lys Ala Arg u Al a 270 275 att tcc tcg cag tcg att ttt Ile Ser Ser n Ser Ile Phe 285 290 atg ccg atc acc cag gct gtc et Pro Ile Thr n Ala Val 305 gta act gac atg att gtg gct Val Thr Asp Net IIe Val Ala 320 tagtcttagg ttgtaagctt caa aca Thr gac Asp ggt G y 260 acc Thr gat Asp t ac Tyr ct c Leu 787 835 883 931 979 1027 1075 1119 <210> 188 <211> 332 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 188 Val Val Ser Val Ser Val et G y Al a 1 5 Ala Lys Val Phe Ser Asp Ala y Asn 25 Arg G u G u Leu Ala Ser Thr lie Arg 40 Tyr Leu Pro Gy lie Thr Leu Pro G u 55 Al a Thr G u Al a Leu G u G y Ala Al a 70 Ser G n Ala Leu Arg G y Asn Leu Al a G n Asp Ala Thr Leu Val Ser Leu Ala 100 105 His Leu Arg IVet Ser G u Val I e Al a 115 120 Ser Arg Ile Ala Val Leu Ser y Pro 130 135 Su Gy n Pro Ala Ala Thr Val lie G y Ser Trp 10 Al a Val Thr Asp Ser Hi s Ser Leu G n l e Val Val G u Trp Lys 90 Lys G y I I e G u Val Thr Asn Leu Al a 140 Al a Cys Pr o Page 244 G y Thr Thr Leu Leu G u Val Leu G u G u G u 125 Ar g Asp Tr p Asn Thr Al a Thr Lys 110 Al a G u G u Al a Ar g Ser I e I e Sy Asp SIe Asn Gene Sequence 24-2-06.txt Al a Thr Val Asn Leu 225 G y Ar g Ar g Ser G n 305 I le Leu Thr Al a 195 Asn Al a Gy Phe Al a 275 Phe Val Al a G n 165 Val Al a Ser Leu Leu 245 G u Asn Leu Gy 3et 325 Al a Val Cys Leu Gy 230 Val Ar g Gy Al a Val 310 Gy Al a Val G y Thr G y lle 200 Ile Thr 215 Al a Asp Al a Thr Leu G y G n Val 280 Thr Lys 295 Cys Hi s Arg Ser Al a Al a 170 G u le 185 Ser His Arg G y Al a Lys Cys Ser 250 G n y 265 Al a G u Leu G y Arg Asp Lys Lys 330 155 Pro Tyr Phe Arg Pro 175 G y G y Al a Cys Lys 190 G y Tyr G y Leu G y 205 Leu Ala G u I e Al a 220 Thr Phe Ser G y Leu 235 Ser Pro Leu Ser Arg 255 G u Ser Leu G u Lys 270 Sy Val lie Ser Ser 285 Val G u let Pro Ile 300 IVet Lys Val Thr Asp 315 Al a u 160 Tyr Asn G u Ar g Al a 240 Asn Al a G n Thr bet 320 <210> 189 <211> 1015 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1015) <223> FRXA01025 <400> 189 gggcagcagc ggcaggtttc cagraggttt ccatgcgggt ctgaracggt taaatatcgt tttcgaaagg tgggtttcgc gt g Val gat Asp agc Ser acg Thr ggt Sy ggc G y at c I e ccg Pr o ggt Sy gct Al a gac Asp tcg Ser tgg Tr p acg Thr cat Hi s cag Sn gga G y ttg Leu gaa G u 45 gt c Val acc Thr tgg Tr p aac Asn aca Thr acg ttg Thr Leu 15 gcg agg Al a Ar g cgt gat Arg Asp t ca t cg Ser Ser Page 245 ggct t ggr ac wt gggct aac gtg gtt tct gta agc Val Val Ser Val Ser 1 gcc aag gtc ttc tct Ala Lys Val Phe Ser cgg gaa gag ttg gca Arg G u G u Leu Ala tac ctt ccg ggg att Tyr Leu Pro Gy lie gca acg gag gct tta Al a Thr G u Ala Leu Gene Sequence 24-2-06.txt gag Gu ggc G y gt g Val gaa Gu ttg Leu gct Al a 150 gct Al a gt g Val tgt Cys ttg Leu ggt Gy 230 gt g Val cgt Ar g ggt Sy gcc Al a gca Al a ttg Leu ttg Leu at c SIle 120 ggg Sy gt g Val gt g Val act Thr at t SIle 200 act Thr gat Asp acg Thr ggt Sy gtt Val 280 aag Lys at t l Ie gag Su aaa Lys gag Su aac Asn gct Al a gcg Al a 170 at c SIle cat Hi s ggc Gy aag Lys t ca Ser 250 ggt Sy gag Su ggt Sy gt g Val 75 tgg Tr p ggt G y gt g Val ctt Leu tgc Cys 155 ccg Pr o ggt Gy ggt Gy ctt Leu act Thr 235 t ca Ser gaa Gu ggt G y gt g Val gtg ttg gcg att cct Val Leu Ala lie Pro 80 aaa gag acg atc ccg Lys G u Thr lie Pro 95 att gaa aag ggc acg I e G u Lys G y Thr 110 acg gaa gcg gat cct Thr G u Ala Asp Pro 125 gct cgt gag atc gcg Ala Arg G u Ile Ala 140 cct gat gaa aac cga Pro Asp G u Asn Ar g 160 tat ttc cgc ccg tac Tyr Phe Arg Pro Tyr 175 ggt gcg tgt aag aac G y Al a Cys Lys Asn 190 tac ggc ctg ggt gag Tyr G y Leu G y G u 205 gca gag atc gca cgc Ala u lie Ala Arg 220 ttt tct ggc ctt gcg Phe Ser G y Leu Al a 240 ccg ct g t cg cgt aac Pro Leu Ser Arg Asn 255 t cc ct a gag aag gct Ser Leu a u Lys Al a 270 gtt att tcc tcg cag Val lie Ser Ser G n 285 gag atg ccg atc acc Su IVet Pro Ile Thr 300 tcg cag gcg ttg cgt Ser G n Ala Leu Arg cag gat gcg acc ttg G n Asp Al a Thr Leu 100 cac ctg cgg atg agt His Leu Arg IVet Ser 115 tca cgc atc gcg gtg Ser Arg Ile Ala Val 130 gag ggg cag cct gca Gu G y Gn Pro Ala 145 gcg aaa ctt gtg cag Ala Lys Leu Val G n 165 acc aac act gat gtg Thr Asn Thr Asp Val 180 gtc atc gcg ctg gcc Val Ile Ala Leu Ala 195 aac acc aat gca tcg Asn Thr Asn Ala Ser 210 ctc ggt gcc aca ttg Leu y Ala Thr Leu 225 gga atg ggc gac ttg Sy IVet y Asp Leu 245 cgc agc ttc ggt gag Arg Ser Phe y G u 260 cgc gag gca acc aat Arg G u Ala Thr Asn 275 tcg att ttt gat ctt Ser Ile Phe Asp Leu 290 cag G n 305 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1015 <210> 190 <211> 305 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 190 Page 246 Gene Sequence 24-2-06. txt Val Val Ser Val Ser Val Met y Al a y Ser Trp y Thr Thr Leu 1 5 10 Ala Lys Val Phe Ser Asp Ala Gy Asn Ala Val Thr Leu Trp Ala Arg 25 Arg G u G u Leu Ala Ser Thr lie Arg Asp Ser His G u Asn Arg Asp 40 Tyr Leu Pro Gy lie Thr Leu Pro u Ser Leu G n Val Thr Ser Ser 55 Ala Thr u Ala Leu G u y Ala Ala Ile Val Val Leu Ala lie Pro 70 75 Ser G n Ala Leu Arg G y Asn Leu Ala G u Trp Lys G u Thr lie Pro 90 G n Asp Ala Thr Leu Val Ser Leu Ala Lys y lie G u Lys y Thr 100 105 110 His Leu Arg IVet Ser G u Val Ile Ala G u Val Thr G u Ala Asp Pro 115 120 125 Ser Arg Ile Ala Val Leu Ser y Pro Asn Leu Ala Arg G u Ile Ala 130 135 140 Gu Gy Gn Pro Ala Ala Thr Val Ile Ala Cys Pro Asp u Asn Arg 145 150 155 160 Ala Lys Leu Val G n Al a Ala Val Ala Ala Pro Tyr Phe Arg Pro Tyr 165 170 175 Thr Asn Thr Asp Val Val y Thr G u Ile y y Ala Cys Lys Asn 180 185 190 Val IIe Ala Leu Ala Cys G y IIe Ser His G y Tyr G y Leu G y G u 195 200 205 Asn Thr Asn Ala Ser Leu Ile Thr Arg y Leu Ala u lie Ala Arg 210 215 220 Leu y Ala Thr Leu G y Ala Asp Ala Lys Thr Phe Ser y Leu Ala 225 230 235 240 G y Iet G y Asp Leu Val Ala Thr Cys Ser Ser Pro Leu Ser Arg Asn 245 250 255 Arg Ser Phe G y G u Arg Leu G y n G y u Ser Leu G u Lys Ala 260 265 270 Arg G u Ala Thr Asn G y n Val Al a Gu Gy Val Ile Ser Ser n 275 280 285 Ser Ile Phe Asp Leu Al a Thr Lys Leu y Val G u Iet Pro lie Thr 290 295 300 G n 305 <210> 191 <211> 1809 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS Page 247 Gene Sequence 24-2-06.txt <222> (101)..(1786) <223> RXA01851 <400> 191 ttgtggcctt tttgcagggg aaacttattt aaataattca cacgatgtgg gttggcggtt ttcctattag gctcactttt t aagt aaaaa accgt caat t atg acg agc gca cac lMt Thr Ser Ala His ttt Phe at t SIle caa G n tct Ser t at Tyr cgc Ar g ttc Phe ggt Gy ggg Gy aat Asn 150 gga Gy ct c Leu aac Asn gt c Val gt g Val t cc Ser at t SIle cgc Ar g aca Thr gag G u t ac Tyr ct c Leu ggt Gy 120 tcg Ser ct g Leu tgg Tr p gcg Al a gcc Al a 200 ggt Gy gcc Al a cgg Ar g ggt Gy tta Leu tcg Ser t ac Tyr ggt Gy gcg Al a gcg Al a gaa G u aag Lys cac Hi s 170 at t l Ie gt c Val gaa G u gt g Val ggc Gy at c SIle act Thr acc Thr ttc Phe gcc Al a gat Asp t at Tyr cac Hi s 140 gt c Val gat Asp gct Al a cgc Ar g act Thr 220 at c SIle cct Pr o t ca Ser gt g Val 45 t cc Ser ggc Gy gct Al a tgg Tr p gaa G u 125 tcg Ser ccg Pr o acg Thr ttt Phe at t SIle 205 gat Asp aac Asn ccg Pr o ggt Gy at g et aag Lys gt g Val ccg Pr o t ca Ser 110 acg Thr ccg Pr o tgg Tr p ttg Leu gcg Al a 190 ct c Leu cag G n gct Al a ctt Leu 15 gt a Val ttt Phe at g et gt c Val cat Hi s 95 gag G u at g Net cgt Ar g ctt Leu aat Asn 175 gca Al a cgg Ar g gt c Val cgc Ar g cag G n gag G u at t SIle cag G n ttg Leu ccg Pr o gcg Al a ttc Phe gac Asp 160 ct c Leu gat Asp aac Asn acc Thr 1 gat Asp at t SIle gcc Al a cat Hi s gaa G u gt g Val aaa Lys tgg Tr p cgg Ar g 145 ccg Pr o cat Hi s ggc Sy gt g Val aac Asn 225 aat Asn gcg Al a aga Ar g ggt Sy gcc Al a gct Al a gcc Al a cag G n 130 tgg Tr p gag G u gca Al a gga Sy gaa G u 210 acc Thr t at Tyr cga Ar g gat Asp ggt Sy gt g Val cca Pr o cct Pr o 115 cgt Ar g at t SIle ggc Sy gaa G u acg Thr 195 gaa G u acg Thr gac Asp cat Hi s t at Tyr ttg Leu aag Lys cgc Ar g 100 at g bet aac Asn cct Pr o ttg Leu cga Ar g 180 gcg Al a ggc Sy cat Hi s gcg ggt ccg tgg gtt gcg cag Ala y Pro Trp Val Ala G n Page 248 Gene Sequence 24-2-06.txt 230 235 240 245 gcg ttg ggt gat ttg gcg gag gta acc aag ttg aag gtg cgc caa tcc 883 Ala Leu y Asp Leu Al a G u Val Thr Lys Leu Lys Val Arg G n Ser 250 255 260 aag gga gtg cat ttg ctc act ggt gat ttg ggc agc cag agt ggc gtg 931 Lys G y Val His Leu Leu Thr G y Asp Leu G y Ser G n Ser G y Val 265 270 275 ttt gtg cgt ggc aaa aac ggc aag cat gtg atc gtg aat ccg tgg atg 979 Phe Val Arg G y Lys Asn G y Lys His Val lie Val Asn Pro Trp IVet 280 285 290 ggg cgc acc ctt att ggt cca acc gac acc atg att gac ggt gac gct 1027 Sy Arg Thr Leu Ile y Pro Thr Asp Thr IVet Ile Asp y Asp Ala 295 300 305 gat gat gcg gct gca gat gaa agc gat atc gat ttg ctg ctt gag acc 1075 Asp Asp Ala Ala Ala Asp G u Ser Asp Ile Asp Leu Leu Leu G u Thr 310 315 320 325 atc gat tcg gta cgc gct aca ccg ctt gat cgc aaa gag atc atc tcc 1123 Ile Asp Ser Val Arg Al a Thr Pro Leu Asp Arg Lys G u lie lie Ser 330 335 340 acg ctg gtg ggt gtg cgc ccg ctt gtt gat gac ggc acc gac acc tac 1171 Thr Leu Val y Val Arg Pro Leu Val Asp Asp y Thr Asp Thr Tyr 345 350 355 acg tcc tct cgc cgt ttc gat att tcc gat cac gcc aac gtc ggc att 1219 Thr Ser Ser Arg Arg Phe Asp IIe Ser Asp His Ala Asn Val G y IIe 360 365 370 gat ggt ttg gtg tct gtc tct ggc ggc aag tgg acc act tcc cgc gtg 1267 Asp y Leu Val Ser Val Ser Gy y Lys Trp Thr Thr Ser Arg Val 375 380 385 atg ggg tac aag gtg att gag cat gtg gtg gag cac caa gct gcg gtg 1315 Net y Tyr Lys Val Ile G u His Val Val G u His G n Ala Ala Val 390 395 400 405 tta cct ccg ctg cgc cac ttt gac tcc agg cag atg ccg ttg agt act 1363 Leu Pro Pro Leu Arg His Phe Asp Ser Arg G n IVet Pro Leu Ser Thr 410 415 420 tct ttt ggc gcg tat gag tcc gtg gct gat tcc ttt gag tca gcg ctt 1411 Ser Phe y Ala Tyr u Ser Val Ala Asp Ser Phe u Ser Ala Leu 425 430 435 cgc agc cac ccc gag ctg gat gtg gat gat gaa atc cgc gtg cat ctg 1459 Arg Ser His Pro G u Leu Asp Val Asp Asp G u Ile Arg Val His Leu 440 445 450 gcc aga ctg tat gga act gag cat gaa aaa gtg ctg gat ctc gtc gca 1507 Ala Arg Leu Tyr G y Thr G u His G u Lys Val Leu Asp Leu Val Ala 455 460 465 aag caa ccc gac ctg ggg cgc cga ctt gac cca gac aac ctt gat atc 1555 Lys G n Pro Asp Leu G y Arg Arg Leu Asp Pro Asp Asn Leu Asp Ile 470 475 480 485 gcg gcg cag gcc gtt ttt gct gtc gcc gag gag gcg gcc gtc gac ctg 1603 Ala Ala G n Ala Val Phe Ala Val Ala G u G u Ala Ala Val Asp Leu 490 495 500 gcg gac gtg ctg gat cgt cgc atc gtg ctc ggc acg ctg ggt tat gtg 1651 Ala Asp Val Leu Asp Arg Arg lie Val Leu y Thr Leu y Tyr Val Page 249 Gene Sequence 24-2-06.txt 505 510 515 caa ccg gct gcc gtg cgt gcg acg gcc gaa gca atg gcg cag gtc acc G n Pro Ala Ala Val Arg Ala Thr Ala G u Ala Net Ala G n Val Thr 520 525 530 ggg tgg tca gct gag ctt atc gac gcc cag tgc cag tcc tac ctc gcc Sy Trp Ser Ala G u Leu Ile Asp Ala G n Cys G n Ser Tyr Leu Ala 535 540 545 aag caa gac aaa atc caa gcc gtg tta aag ccg tac cgc taacactccg Lys G n Asp Lys Ile G n Ala Val Leu Lys Pro Tyr Arg 550 555 560 tcatcgacac cgg <210> 192 <211> 562 <212> PRT <213> Corynebacter i um gl utam cum 1699 1747 1796 1809 <400> 192 Met Thr Ser Ala His Phe G u Ser 1 Asp SIle Al a Hi s G u Val Lys Tr p Ar g 145 Pr o Hi s Sy Val Asn 225 Tyr Ar g Asp Sy Val Pr o Pr o 115 Ar g SIle Sy G u Thr 195 G u Thr 5 Val Al a Ser Ar g G u Ser Leu G n Lys Lys 165 Leu SIle Ar g G u Val Sy Sy Leu Ar g et Al a G n 135 Al a Al a Leu Hi s Lys 215 Asn SIle Ar g 40 Thr G u Tyr Leu Sy 120 Ser Leu Tr p Al a Al a 200 G y Al a Arg Arg Il e 10 Sy y y G y Leu Arg Ser Ser Thr G n Tyr Asp Leu G y Il e 90 Thr Al a Phe 105 Val Al a Leu Lys G u Asn Leu Lys G u 155 Arg His Asp 170 Val Ile Lys 185 Lys Val Thr Val G u Val Pro Val Val 235 Pr o Ser Val Ser Sy Al a Tr p G u 125 Ser Pr o Thr Phe SIle 205 Asp Leu Val Phe bet Val Hi s G u et Ar g Leu Asn 175 Al a Ar g Val Ile Asn Ala Ala Page 250 Pro Trp Val Ala G n Ala Leu 245 Gene Sequence 24-2-06.txt Sy Asp Leu Ala G u Val Thr Lys Leu 255 Lys Ser Val SIle 305 Leu Lys Gy Al a Thr 385 Hi s let Phe SIle Leu 465 Asp Al a Thr vet G n 545 Tyr Ar g Ser 275 Pr o Sy Leu SIle Asp 355 Val Ser Al a Leu Ser 435 Val Leu Leu Val Sy 515 G n Ty r Ser Val let Al a Thr 325 Ser Tyr SIle Val Val 405 Thr Leu Leu Al a SIle 485 Leu Val Thr Al a Lys Phe Sy Asp 310 I le Thr Thr Asp bet 390 Leu Ser Ar g Al a Lys 470 Al a Al a G n Sy Lys 550 Sy Val Ar g 295 Asp Asp Leu Ser Sy 375 Sy Pr o Phe Ser Ar g 455 G n Al a Asp Pr o Tr p 535 G n Val Ar g 280 Thr Al a Ser Val Ser 360 Leu Tyr Pr o Sy Hi s 440 Leu Pr o G n Val Al a 520 Ser Asp Hi s 265 Sy Leu Al a Val Sy 345 Ar g Val Lys Leu Al a 425 Pr o Tyr Asp Al a Leu 505 Al a Al a Lys Leu Asn Sy Asp 315 Al a Ar g Phe Val SIle 395 Hi s G u Leu Thr Sy 475 Phe Ar g Ar g Leu G n 555 Thr Sy Pr o 300 G u Thr Pr o Asp Ser 380 G u Phe Ser Asp G u 460 Ar g Al a Ar g Al a SIle 540 Al a Sy Lys 285 Thr Ser Pr o Leu SIle 365 Sy Hi s Asp Val Val 445 Hi s Ar g Val SIle Thr 525 Asp Val Asp 270 Hi s Asp Asp Leu Val 350 Ser Sy Val Ser Al a 430 Asp G u Leu Al a Val 510 Al a Al a Leu Leu Val Thr SIe Asp 335 Asp Asp Lys Val Ar g 415 Asp Asp Lys Asp G u 495 Leu G u G n Lys <210> 193 <211> 900 <212> DNA <213> Cor ynebact eri um gl ut am cum Page 251 Gene Sequence 24-2-06.txt <220> <221> CDS <222> (101)..(877) <223> RXA01242 <400> 193 cgccggcaac caaatgaggc ttttgggcgt tggacagtga gacaatgggt aagaaattcg gacatattta gtaaattggc tttttgcttt aaggagtgac atg bet tac gca gag gag Tyr Ala G u G u cgc Ar g gt c Val cga Ar g ggt Gy gat Asp gca Al a gcg Al a agc Ser aat Asn gtt Val 150 ct c Leu acg Thr aaa Lys t cc Ser cga Ar g gaa G u ctt Leu gca Al a cgt Ar g at g let act Thr aag Lys 120 gcc Al a gcg Al a ct g Leu gat Asp gcg Al a 200 aag Lys at t I e gcg Al a gt g Val gcc Al a agg Ar g ttc Phe gtt Val tgg Tr p gcc Al a acc Thr cgt Ar g 170 gga G y at c l Ie ggc G y gcc Al a ggc G y ct a Leu acc Thr tct Ser 75 ct g Leu act Thr tcg Ser ggg G y cag G n 155 gcg Al a tt g Leu acc Thr acc Thr t ca Ser cga Ar g gac Asp caa G n gca Al a ccc Pr o gct Al a at c SIle ctt Leu 140 gct Al a gat Asp tct Ser aac Asn gac Asp 220 tta Leu ttc Phe cgc Ar g 45 tct Ser tcg Ser gct Al a ttg Leu gt g Val 125 gat Asp gtt Val gt a Val acg Thr gcc Al a 205 t ac Tyr acg Thr gat Asp gag G u ttc Phe t ca Ser gag G u gcc Al a 110 acc Thr gat Asp gt g Val gt g Val gcc Al a 190 cac Hi s ct c Leu gca Al a 15 gt c Val gga G y caa G n gca Al a cat Hi s 95 gat Asp aac Asn gt c Val ggt G y ttc Phe 175 gat Asp aag Lys gtt Val act Thr at t SIle acc Thr aag Lys ggc Gy ct c Leu tgc Cys cag G n gac Asp 160 at c SIle t cc Ser gt g Val 1 gag G u gca Al a gtt Val aca Thr t ac Tyr gga G y at t SIle ct c Leu ct g Leu 145 act Thr ggc G y caa G n gt g Val gga G y gag G u cac Hi s gag G u t cc Ser ct g Leu tct Ser ccc Pr o 130 ctt Leu gcg Al a acc Thr gag G u gt g Val 210 cgt Ar g acg Thr cgc Ar g ttg Leu at t SIle ttc Phe gag G u 115 at c SIle gga G y ctt Leu aac Asn gct Al a 195 ttg Leu gta Val att SIe gtt Val agc Ser gcc Al a ct c Leu 100 cat Hi s gca Al a gga G y cgt Ar g gcg Al a 180 gcc Al a tgt Cys gtg agc ttt ggc gca atc agc Val Ser Phe G y Ala lie Ser 225 Page 252 Gene Sequence 24- 2- 06. txt gat atc gat gtg gtg gtc acc gat gcg ggt gca cca gca agt ttc gtt 835 Asp Ile Asp Val Val Val Thr Asp Ala Gy Ala Pro Ala Ser Phe Val 230 235 240 245 gag cag ttg cga gaa cgc gat gta gaa gtt gtg att gca gaa 877 Su G n Leu Arg u Arg Asp Val u Val Val i e Ala G u 250 255 tgattcttac agtcactgca agt 900 <210> 194 <211> 259 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 194 IVet Tyr Ala G u G u Arg Arg Arg G n I e Ala Ser Leu Thr Ala Val 1 5 10 G u y Arg Val Asn Val Thr G u Leu Ala y Arg Phe Asp Val Thr 25 Ala u Thr Ile Arg Arg Asp Leu Ala Val Leu Asp Arg G u y lie 40 Val His Arg Val His G y G y Ala Val Ala Thr G n Ser Phe G n Thr 55 Thr G u Leu Ser Leu Asp Thr Arg Phe Arg Ser Ala Ser Ser Ala Lys 70 75 Tyr Ser I e Al a Lys Al a Al a Iet G n Phe Leu Pro Al a G u Hi s G y 90 Sy Leu Phe Leu Asp Al a y Thr Thr Val Thr Ala Leu Ala Asp Leu 100 105 110 Ile Ser G u His Pro Ser Ser Lys G n Trp Ser lie Val Thr Asn Cys 115 120 125 Leu Pro lie Ala Leu Asn Leu Ala Asn Ala y Leu Asp Asp Val G n 130 135 140 Leu Leu y y Ser Val Arg Ala Ile Thr G n Ala Val Val y Asp 145 150 155 160 Thr Ala Leu Arg Thr Leu Ala Leu Iet Arg Ala Asp Val Val Phe Ile 165 170 175 G y Thr Asn Ala Leu Thr Leu Asp His G y Leu Ser Thr Ala Asp Ser 180 185 190 Sn u Ala Ala IVet Lys Ser Ala IVet lie Thr Asn Ala His Lys Val 195 200 205 Val Val Leu Cys Asp Ser Thr Lys IVet G y Thr Asp Tyr Leu Val Ser 210 215 220 Phe y Ala lie Ser Asp Ile Asp Val Val Val Thr Asp Ala y Ala 225 230 235 240 Pro Ala Ser Phe Val G u G n Leu Arg G u Arg Asp Val G u Val Val 245 250 255 Ile Ala G u Page 253 Gene Sequence 24-2-06.txt <210> 195 <211> 969 <212> DNA <213> Corynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(946) <223> RXA02288 <400> 195 aacaacaatc taacgccatc atgttataaa aaagcaagac agtgctggat ctaacaacat ttccgtggta actttttcac ctaacataaa aatgttagaa atg tcc caa gtg att 11! let Ser G n Val lie ccc Pr o acc Thr gt c Val aat Asn at g Met aaa Lys gt g Val ttg Leu at g Met gcg Al a 150 gca Al a tct Ser agc Ser cat Hi s gca Al a gt g Val gag G u gcg Al a gcg Al a cag G n 120 gat Asp gcg Al a t ca Ser act Thr caa G n ttc Phe acc Thr cgc Ar g gca Al a tgt Cys gat Asp t ca Ser cgt Ar g ttg Leu cag G n 170 gt g Val gaa G u gct Al a at t SIle at t SIle gt g Val 75 act Thr gat Asp cca Pr o gt g Val t at Ty r 155 tt g Leu cgc Ar g aag Lys cgt Ar g cac Hi s agg Ar g gag G u gca Al a t cc Ser tcg Ser cgc Ar g 140 ttc Phe aat Asn gct Al a cgt Ar g gtt Val cgt Ar g 45 ggt G y cag G n gca Al a acc Thr gcg Al a 125 gcc Al a gcg Al a gag G u acg Thr cgt Ar g gaa G u gat Asp ggc G y cgt Ar g gca Al a acg Thr 110 ttg Leu ggg Gy gag G u ct g Leu ggg G1y 190 gag G u 15 gca Al a ttg Leu gcg Al a cgg Ar g cgg Ar g 95 ttg Leu at t SIle at g let act Thr tcg Ser 175 gag G u cgc Ar g tta Leu gag G u cgt Ar g cat Hi s 80 ttg Leu gag G u acg Thr agc Ser gat Asp 160 gcg Al a gtt Val 1 at c SIle gct Al a gcg Al a tcg Ser ttg Leu at t SIle tct Ser cat Hi s gat Asp 145 tct Ser gat Asp ccg Pr o gtt Val gag G u ct g Leu gt g Val cat Hi s ccg Pr o ttg Leu tct Ser 130 at c SIle ttc Phe at t SIle gcg Al a t at Tyr ttt Phe gca Al a ccg Pr o act Thr ggc G y 100 gag G u aag Lys ttg Leu ggc Gy ttt Phe 180 ttt Phe gtc Val gag G u gac Asp tcg Ser gtt Val gct Al a aag Lys aca Thr att SIe aag Lys 165 gtt Val cat Hi s cct gat atg gag gct gct gat acg aag cgg gcg ttg att ggg att ggt Page 254 Pr o agc Ser gt g Val 230 cag G n cag G n acg Thr Met 200 cgt Ar g aag Lys tgc Cys cat Hi s gag G0u 280 Al a ttg Leu gct Al a cgt Ar g 250 at t SIle ttt Phe Gene Sequence 24-2-06.txt Al a Asp Thr Lys Arg Ala Leu Ile y 205 210 gtg gtg gat tct agt aaa ttt ggt tcg Val Val Asp Ser Ser Lys Phe G y Ser 220 225 tcg att gag gag ttt gac cac atc atc Ser Ile u u Phe Asp His Ile Ile 235 240 gag cag cgg gat ctt ttg cgt aat tcg G u G n Arg Asp Leu Leu Arg Asn Ser 255 gac cac aat ggt gat gaa att ttg gat Asp Hi s Asn G y Asp G u IIe Leu Asp 270 275 taagatggct ttggttcttg gaa SIe gct Al a att SIe cgc Ar g 260 acc Thr <210> 196 <211> 282 <212> PRT <213> Corynebacter i um gl ut ai cum Net 1 I e Al a Al a Ser Leu SIle Ser Hi s Asp 145 Ser Asp Pr o 0> 196 Ser G n Val Val Ser Tyr 2C G u Leu Phe Leu Al a Al E Val Ser Pr c His Arg Thr Pro G u G Leu Val G L 115 Ser Leu Lys 130 SI e Ar g LeL Phe Leu G Ile Ser Phe 18C Al a Leu Phe 195 Ile Pro 5 Val Thr G u Val Asp Asn Ser Mbet 70 Val Lys Al a Val Lys Leu Thr Mbet I e Al a 150 Lys Al a 165 Val Ser Hi s Pr o Al a Ar g Ser Leu 55 Ser G u Val Pr o Al a 135 Cys Thr Thr Asp Ser Hi s Al a 40 Val G u Al a Al a G n 120 Asp Al a Ser Thr \et 200 Ser Sy bet G u Leu Leu l Ie 105 Ar g Hi s Sy Al a Al a 185 G u G u Al a SIle SIle Val Thr Asp Pr o Val Tyr 155 Leu Ar g Lys Ar g Hi s Ar g G u Al a Ser Ser Ar g 140 Phe Asn Al a Ar g Val Ar g Sy G n Al a Thr Al a 125 Al a Al a G u Thr G u Al a Leu Al a Ar g Ar g Leu SIe Net Thr Ser 175 G u Ala Ala Asp Thr Lys Arg Ala 205 Page 255 Leu lI e G y l e G y 210 Phe G y Ser Al a G y 225 His Ile Ile Ile Asp 245 Arg Asn Ser Arg Ala 260 Ile Leu Asp Thr Pro 275 Ser Val 215 Val Phe 230 Gn i n Thr n I I e Thr 3 u Gene Sequence 24-2-06.txt Arg Val Leu Val Val Asp Ser Ser Lys 220 Lys Val Ala Ser Ile G u G u Phe Asp 235 240 Cys Thr Arg G u G n Arg Asp Leu Leu 250 255 His Val lie Asp His Asn G y Asp G u 265 270 G u Asp Phe 280 <210> <211> <212> <213> <220> 197 887 DNA Cor ynebact eri um gl ut ari cum <221> CDS <222> <223> RXN01891 <400> 197 ggt ggc cac G y G y Hi s 1 tac aac aag Tyr Asn Lys gag tca t gg Su Ser Trp atg gac agt IVet Asp Ser tct tgg act Ser Trp Thr gaa ggt tgg G u G y Trp gag tgg ctc G u Trp Leu gac gtc acc Asp Val Thr 115 t cc acc ggt Ser Thr G y 130 ggc gta gca G y Val Ala 145 ggt Sy 5 ct g Leu gag G u ttc Phe gaa G u t cc Ser t cc Ser gag G u ct g Leu ctt Leu tt g Leu tgg Tr p tt c Phe gca Al a ggc Sy 70 cgt Ar g acc Thr tt c Phe tct Ser cct Pr o 150 cct Pr o gct Al a t cc Ser cac Hi s 55 cca Pr o ct g Leu gtt Val gca Al a tcg Ser 135 aac Asn ttc Phe aag Lys gag G u 40 ggt Sy at g bet act Thr gat Asp acc Thr 120 gtt Val cca Pr o gct Al a gct Al a tgg Tr p tgg Tr p tgg Tr p acc Thr gaa G u 105 ggc G y gcc Al a acc Thr t cc Ser ct g Leu cca Pr o gat Asp ct c Leu gag G u ttc Phe at c SIle gct Al a gag 15u 155 act Thr gaa G u aag Lys gca Al a ggc Sy acc Thr gca Al a ggt G y gca Al a 140 ggc G y gt c Val gat Asp ct g Leu acc Thr ggc Sy at c SIle acc Thr t ca Ser 125 agc Ser gct Al a ttc Phe ggc Sy gaa G u t ac Tyr t ac Tyr gca Al a t cc Ser at c SIe gac Asp cca Pr o Page 256 Gene Sequence 24-2-06.txt ggt ggc gca ggc ctg gga atc cca tct Oy Oy Ala y Leu Oy Ile Pro Ser ggc atc tct gag cag cgt cag Gy lle Ser u n Arg n 170 175 gac Asp ggc Sy gca Al a aac Asn 225 cgc Ar g aag Lys gag G u aac gcc Asn Ala tac tgg Tyr Trp 195 tct gat Ser Asp 210 gtt gca Val Ala gtg ct g Val Leu atc tgc I e Cys acc aag Thr Lys 275 165 ctg aag ttc atc Leu Lys Phe Il e 180 tcc cgc gag acc Ser Arg G u Thr cca gat cac gca Pro Asp His Ala 215 gtg gag cag ctt Val G u G n Leu 230 ctg cca aac ggt Leu Pro Asn y 245 ctg act ggt gca Leu Thr G y Al a 260 ctg aac acc atc Leu Asn Thr Ile gac Asp ggt Gy 200 gca Al a cct Pr o gac Asp gac Asp t ac Tyr 280 ttc Phe 185 t at Tyr ttc Phe gat Asp cgc Ar g at c SIle 265 acc Thr acc Thr cca Pr o gag G u cgt Ar g 235 at c SIle gt c Val gac Asp taatccgagc acttcagcta ca <210> 198 <211> 288 <212> PRT <213> Corynebacter i um gl <400> 198 G y G y Hi s Tyr G y Leu 1 5 Tyr Asn Lys Asp Leu Trp Su Ser Trp u u Phe IVet Asp Ser G y Phe Al a Ser Trp Thr Phe G u G y 70 G u G y Trp u Ser Ar g G u Trp Leu Lys Ser Thr 100 Asp Val Thr Asn G u Phe 115 Ser Thr G y Asp Leu Ser 130 .c gcc Al a cgt Ar g 205 aac Asn cag G n gac Asp ct g Leu gaa G u 285 Val Asp Leu Thr Sy SIle Thr Ser 125 Ser gcg Al a 190 aag Lys cct Pr o gac Asp gca Al a gct Al a 270 cca Pr o Leu Ar g G n Asn Asn Ar g Val 110 Cys Phe aac Asn gat Asp gca Al a aac Asn ct g Leu 255 gag Gu ctt Leu Phe Sy G u Tyr Tyr Al a Ser SIle Asp act Thr gct Al a t ac Tyr ttc Phe 240 gag G u gtt Val att SIe Tyr Pr o Al a Leu Ser Val Thr G n Tr p ut ari cum Pro Phe Al a Al a Lys Al a 25 Ser G u Trp 40 His G y Trp 55 Pro IVet Trp Leu Thr Thr Val Asp G u 105 Al a Thr G y 120 Ser Val Al a 135 Arg Ser Thr 10 G y Leu G u G y Pro Lys G y Asp Al a Ser Leu G y 75 Pro G u Thr 90 G y Phe Al a Leu I le G y G y Al a Al a 140 Page 257 Gene Sequence 24-2-06.txt Sy Val Ala Ala Leu Pro Asn Pro Thr Gy G u y Ala Cys Pro Thr 145 150 155 160 G y G y Al a G y Leu G y Ile Pro Ser G y Ile Ser G u n Arg n 165 170 175 Asp Asn Ala Leu Lys Phe Ile Asp Phe Leu Thr Asn Ala Ala Asn Thr 180 185 190 Sy Tyr Trp Ser Arg G u Thr y Tyr Val Pro Val Arg Lys Asp Ala 195 200 205 Ala Ser Asp Pro Asp His Ala Ala Phe Leu G u G u Asn Pro Ala Tyr 210 215 220 Asn Val Ala Val G u G n Leu Pro Asp Thr Arg Ser G n Asp Asn Phe 225 230 235 240 Arg Val Leu Leu Pro Asn y Asp Arg Thr Ile y Asp Ala Leu G u 245 250 255 Lys Ile Cys Leu Thr G y Ala Asp Ile Asp Val Thr Leu Ala G u Val 260 265 270 G u Thr Lys Leu Asn Thr Ile Tyr Thr Arg Asp lie G u Pro Leu Ile 275 280 285 <210> 199 <211> 842 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> FRXA01891 <400> 199 tac tac aac aag gat ctg tgg gct aag gct ggc ctg gaa gat cgt ggc 48 Tyr Tyr Asn Lys Asp Leu Trp Ala Lys Ala y Leu G u Asp Arg y 1 5 10 cca gag tca tgg gaa gag ttc tcc gag tgg ggt cca aag ctg cag gaa 96 Pro u Ser Trp G u G u Phe Ser G u Trp y Pro Lys Leu n G u 25 gcg atg gac agt ggt ttc gca cac ggt tgg gga gat gca acc aac tac 144 Al a IVet Asp Ser G y Phe Al a Hi s G y Trp G y Asp Al a Thr Asn Tyr 40 ctt tct tgg act ttc gaa ggc cca atg tgg tcc ctc ggc ggc aac tac 192 Leu Ser Trp Thr Phe G u y Pro IVet Trp Ser Leu y y Asn Tyr 55 t ct gaa ggt t gg gag t cc cgt ct g act acc cca gag acc at c cgt gca 240 Ser G u y Trp u Ser Arg Leu Thr Thr Pro G u Thr Ile Arg Ala 70 75 gtt gag tgg ctc aag tcc acc gtt gat gaa ggt ttc gca acc gtc tcc 288 Val G u Trp Leu Lys Ser Thr Val Asp G u y Phe Ala Thr Val Ser 90 acc gac gtc acc aac gag ttc gca acc ggc ctg atc ggt tca tgc atc 336 Thr Asp Val Thr Asn G u Phe Ala Thr y Leu Ile y Ser Cys Ile Page 258 Gene Sequence 24-2-06.txt cag G n tgg Tr p acc Thr 145 cag G n act Thr gct Al a t ac Tyr ttc Phe 225 gag G u gtt Val at t SIe t cc acc Ser Thr 115 ggc gta G y Val 130 ggt ggc G y G y gac aac Asp Asn ggc tac G y Tyr gca t ct Al a Ser 195 aac gtt Asn Val 210 cgc gtg Arg Val aag at c Lys II e gag acc G u Thr gat Asp gca Al a ggc Gy ct g Leu 165 t cc Ser cca Pr o gt g Val ct g Leu ct g Leu 245 ct g ct g Leu ctt Leu ct g Leu 150 aag Lys cgc Ar g gat Asp gag G u cca Pr o 230 act Thr aac tct Ser cct Pr o 135 gga Gy ttc Phe gag G u cac Hi s cag G n 215 aac Asn ggt Gey acc tcg Ser 120 aac Asn at c SIle at c SIle acc Thr gca Al a 200 ctt Leu ggt Gy gca Al a at c 105 gtt Val cca Pr o cca Pr o gac Asp ggt Gy 185 gca Al a cct Pr o gac Asp gac Asp t ac Tyr 265 gcc Al a acc Thr tct Ser ttc Phe 170 t at Tyr ttc Phe gat Asp cgc Ar g at c SIle 250 ggc Gy ggc Gy ggc Gy 155 ct c Leu gtt Val ct c Leu acc Thr acc Thr 235 gat Asp gct Al a gag G u 140 at c SIle acc Thr cca Pr o gag G u cgt Ar g 220 at c SIle gt c Val gca Al a 125 ggc Gy tct Ser aac Asn gtt Val gag G u 205 t cc Ser ggt Gy acc Thr ttc Phe tgc Cys cag G n gcg Al a 175 aag Lys cct Pr o gac Asp gca Al a gct Al a 255 acc cgc gac atc gaa cca ctt Thr Arg Asp lie u Pro Leu Leu Asn Thr Ile taatccgagc acttcagcta cac <210> 200 <211> 273 <212> PRT <213> Corynebacter i um gl ut am cum <400> 200 Tyr Tyr Asn Lys Asp Leu Trp Ala Lys 1 5 Pro u Ser Trp G u G u Phe Ser G u 25 Al a Vet Asp Ser G y Phe Ala His G y 40 Leu Ser Trp Thr Phe u y Pro IVet 55 Ser G u y Trp u Ser Arg Leu Thr 70 Ala G y Leu G u Asp Arg G y 10 Trp y Pro Lys Leu n G u Trp y Asp Ala Thr Asn Tyr Trp Ser Leu Gy y Asn Tyr Thr Pro G u Thr lie Arg Ala 75 Page 259 Val G u Trp Leu Lys Ser Thr Gene Sequence 24-2-06.txt Val Asp G u y Phe Ala Thr Val Ser 90 Thr G n Tr p Thr 145 G n Thr Al a Tyr Phe 225 G u Val SIe Val Thr 115 Val Gy Asn Tyr Ser 195 Val Val SIle Thr Asn Asp Al a Gy Leu 165 Ser Pr o Val Leu Leu 245 Leu Phe Ser Pr o 135 Gy Phe G u Hi s G n 215 Asn Gy Thr Al a Ser 120 Asn SIle SIle Thr Al a 200 Leu Gy Al a I le Thr 105 Val Pr o Pr o Asp Gy 185 Al a Pr o Asp Asp Tyr 265 Gy Al a Thr Ser Phe 170 Tyr Phe Asp Ar g SIle 250 Thr Leu Gy Gy Gy 155 Leu Val Leu Thr Thr 235 Asp Ar g SIle Al a G u 140 I le Thr Pr o G u Ar g 220 I le Val Asp Gy Al a 125 Gy Ser Asn Val G u 205 Ser Gy Thr SIle Ser 110 Ser Al a G u Al a Ar g 190 Asn G n Asp Leu G u 270 Cys Phe Cys G n Al a 175 Lys Pr o Asp Al a Al a 255 Pr o <210> <211> <212> <213> <220> <221> <222> <223> <400> tttacc 201 776 DNA Cor ynebact eri um gl ut am cum CDS (753) RXA02414 201 Satg agt tac aac agc ccg tat aac aac acg aat ttc agc acc act 51 IVet Ser Tyr Asn Ser Pro Tyr Asn Asn Thr Asn Phe Ser Thr Thr 1 5 10 :g ttc caa cct gct ggt ggg ccg gtg aag ccg tgg aat aag ccc 99 a Phe G n Pro Al a y y Pro Val Lys Pro Trp Asn Lys Pro 25 :c agc ctg aat cag cag ctg aaa aac aaa tcc cgt gtg cgc aca 147 a Ser Leu Asn n G n Leu Lys Asn Lys Ser Arg Val Arg Thr 40 t acc atc gcc atc ggt tat gta gtg gtg att tgg gcg gtg cat 195 ,u Thr IIe Ala II e G y Tyr Val Val Val IIe Trp Ala Val His 55 :a tcc atc gtc att gcg ctg ctc act ggc ttc aac ctg acc aac 243 Page 260 ggc G y gat Asp ggt tgy ttg Leu Ala Ser Ile Val I le Al a Gene Sequence 24-2-06.txt Leu Leu Thr G y Phe Asn Leu Thr Asn ttt Phe t ca Ser cca Pr o ttc Phe tgg Tr p at t SIle 160 gat Asp ggt Sy ggc G y gcc Al a at t SIle ct g Leu ttt Phe gaa G u 130 ttc Phe ggc Gy aaa Lys ttc Phe ctt Leu 210 gat Asp ccg Pr o cat Hi s 100 ttc Phe acg Thr gga Gy ctt Leu ttc Phe 180 ggt Sy ggt Sy ccg Pr o ct g Leu 85 gga Gy agt Ser at t SIle at c SIle ggc Gy 165 ct g Leu ct g Leu gca Al a gca Al a gat Asp agc Ser ttc Phe at c SIle ggc Gy 150 t ac Tyr ctt Leu ct a Leu ctt Leu gcg Al a 230 agt Ser agc Ser at c SIle 120 ggt Gy aac Asn at c SIle at a SIle act Thr 200 gga G y aaa Lys gca Al a cac Hi s 105 ggt Gy ct c Leu cac Hi s gt g Val gtt Val 185 caa G n at c SIle gcg Al a ct g Leu ct c Leu at g bet at c SIle at c SIle cgt Ar g 170 ttg Leu at c SIle ggt G y aag tgg Tr p at t SIle agt Ser ggc Gy ggt Gy 155 gga Gy gcg Al a ggt G y gcg Al a aag ggt Gy gga Gy a ggc Gy ggt GI y 140 gcg Al a at t lie ttc Phe gt g Val ggt G y 220 caa at t SIle aat Asn aag Lys 125 ctt Leu t cc Ser ttc Phe at t SIle t cc Ser 205 gct Al a cag ttc Phe acc Thr 110 cgc Ar g ggt Gy ggc Gy aac Asn t ac Tyr 190 tgg Tr p ttt Phe aag Lys Lys 235 G n G n Lys Lys tta gaa aag caa caa cgc caa aga ggc tta tagttttcac ctagcgacta Leu Gu Lys On On Arg On Arg Gy Leu 240 245 cac <210> 202 <211> 249 <212> PRT <213> Corynebacter i um gl utam cum <400> 202 IVet Ser Tyr Asn Ser Pro Tyr Asn Asn Thr Asn Phe Ser Thr Thr Gy 1 5 10 Ala Phe G n Pro Ala G y G y Pro Val Lys Pro Trp Asn Lys Pro Asp 25 Ala Ser Leu Asn n G n Leu Lys Asn Lys Ser Arg Val Arg Thr y 40 Leu Thr Ile Al a lie y Tyr Val Val Val I e Trp Ala Val His Leu 55 Page 261 Ala Ser Ile Val I e Al a Leu Gene Sequence 24-2-06.txt Leu Thr G y Phe Asn Leu Thr Asn Phe Gy Pr o Gy Tr p SIle 145 Tyr SIle Leu Hi s Ser 225 G u Hi s Leu SIle 115 Val Gy Tr p G n Tr p 195 Phe Asp G n Leu Gy Ser I e I e Gy 165 Leu Leu Al a Al a Ar g 245 Asp Ser Phe SIle Gy 150 Ty r Leu Leu Leu Al a 230 G n Thr Phe Leu Al a 135 Thr Leu Gy Pr o Gy 215 Leu Ar g Al a Hi s 105 Gy Leu Hi s Val Val 185 G n SIle Al a Leu Tr p SIle Ser Gy Gy 155 Gy Al a Gy Al a Lys 235 SIle Asn Lys 125 Leu Ser Phe l Ie Ser 205 Al a G n Thr Val Val Thr Leu Lys 175 Ser G n SIe Lys <210> 203 <211> 840 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(817) <223> RXN01580 <400> 203 cggtaaacgc ctcattaaag tccaatgcca tgctcataac tcaactttgc tccctatcct taaaaagccc acagaaaagg cac Hi s at g Met at c SIle gca Al a gtt Val gct Al a gcc Al a cgt Ar g cat Hi s cag G n gct Al a gcc Al a cgc Ar g gct Al a gac Asp gcg Al a ggt Sy gcc Al a aat Asn cca Pr o gcg Al a gcg Al a cag G n 45 gat Asp gaa G u ccc Pr o gt c Val t cc Ser gat ctg Asp Leu 15 gct gac Al a Asp gtc gtt Val Val ctg cac Leu Hi s Page 262 actaacagtt aaccgtgcgg atg tat aag aat atg IVet Tyr Lys Asn IVet 1 cac ctc gaa aac acc His Leu G u Asn Thr gct ttt gag ctg gat Ala Phe G u Leu Asp atc cac gac cgc acc lie His Asp Arg Thr cgc gac acc ccg gtt Arg Asp Thr Pro Val Gene Sequence 24-2-06.txt gcg Al a t cc Ser at c l Ie tta Leu ttc Phe cgc Ar g 150 gat Asp gca Al a gtt Val caa G n tta Leu gt a Val gt g Val cag G n 120 gat Asp gga Gy at c SIle aac Asn tgc Cys 200 ggc Gy gcc Al a acc Thr at c SIle t ac Tyr gca Al a ttg Leu ct a Leu 170 gcg Al a aca Thr gat Asp gcg Al a 75 ct g Leu aaa Lys cca Pr o ct g Leu cgc Ar g 155 aaa Lys t ca Ser at t SIle tac Ty r caa G n gag G u tct Ser gag G u gt g Val 140 gat Asp aat Asn at t SIle cgg Ar g gcc Al a 220 at c SIle gaa G u gcc Al a cac Hi s 125 gaa G u gcg Al a gt g Val gct Al a gat Asp 205 act Thr aag Lys gtt Val ggt Gy 110 ct g Leu at c SIle t cc Ser ggc Gy gat Asp 190 gaa G u gtt Val gag G u ct a Leu 95 gca Al a gag G u gt g Val at g bet gcg Al a 175 ct a Leu aat Asn agc Ser ata acg Ile Thr 80 ctc cag Leu G n gtt cca Val Pr o cgc ctg Arg Leu gat cga Asp Arg 145 gat gat Asp Asp 160 atc ttg II e Leu cat acc Hi s Thr gcg ttt Al a Phe gat ccc Asp Pro 225 ctt Leu acg Thr gca Al a ct g Leu 130 ct g Leu ct g Leu ccc Pr o aag Lys ggg Gy 210 tct Ser at c SIle agc Ser gcc Al a 115 ttc Phe cca Pr o cgc Ar g tcg Ser gga Gy 195 at c SIle cgt Ar g gac Asp ct g Leu 100 gca Al a at c SIle gaa G u at t SIle tgg Tr p 180 at c SIle gca Al a ttc gga Gy ccg Pr o gca Al a agt Ser gct Al a ctt Leu 165 aaa Lys aag Lys caa G n ct c Phe Leu gcg ccc tcc cct gct ggg gag ttg cac Ala Pro Ser Pro Ala G y u Leu His 230 235 ctg <210> 204 <211> 239 <212> PRT <213> Corynebacter i um gl utam cum <400> 204 IVet Tyr Lys Asn IVet His Ile Val Ala 1 5 His Leu G u Asn Thr IVet Thr Ala Phe 25 Ala Phe G u Leu Asp I e His Ala Thr 40 lie His Asp Arg Thr Ala Ala Arg Val 55 Arg Asp Thr Pro Val Al a Arg Leu Ser tgg taaataatct agtgaccaga Tr p His Arg Gy Ala u Asp Leu 10 G n Ala Ala Ala Pro Ala Asp Ala Asp Asn n Val Val Val Ala Ala Pro Asp Ser Leu His Ala Ala n Ile Lys G u I I e Page 263 Gene Sequence 24-2-06.txt Thr Leu lie Asp G y Ser Pro Val Pro Thr Leu 90 G n Thr Ser Leu Pro Ile G n Val G u I e Lys 100 105 Pro Ala Ala Ala Ala Leu Leu G n Lys Tyr Pro 115 120 Leu Leu Phe Ile Ser Phe Ile Asp Ala Ala Leu 130 135 Arg Leu Pro G u Ala Arg Val Gy Ile Leu Arg 145 150 155 Asp Leu Arg lie Leu Asp Tyr Ile Pro Leu Lys 165 170 Leu Pro Ser Trp Lys Ala Leu Asn Val Ala Ser 180 185 Thr Lys Gy Ile Lys Val y Cys Trp Thr Ile 195 200 Phe Gy Ile Al a Gn n Al a y Val Asp Tyr 210 215 Pro Ser Arg Phe Leu Al a Pro Ser Pro Ala y 225 230 235 <210> 205 <211> 1314 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1291) <223> RXA01436 <400> 205 gcctaaacaa accagtcaac gacctttccc gtggcgcaac cagtagccat cacagcaatt caggcaggag gacgcagcta G u G u Val Ser G u Val 140 Asp Asn SIle Ar g Al a 220 G u Al a Hi s 125 G u Al a Val Al a Asp 205 Thr Leu Leu Leu Al a Val G u Arg Val Asp IVet Asp 160 Ala I e 175 Leu Hi s Asn Ala Ser Asp Tr p agtccctgac atcgtcaaca atg gca ttg gca ctt gtt Val gaa G u at t l Ie t at Tyr gcg Al a aac Asn tct Ser gag G u ct a Leu gat Asp ggt Gy at c SIle aac Asn aca Thr at g bet t ca Ser gac Asp ggc Gy ccc Pr o gac Asp tct Ser gag G u cgc Ar g at c SIle cag G n t cc Ser cca Pr o at c SIle 45 gca Al a cac Hi s at c SIle t at Tyr gt a Val gat Asp aac Asn IVet Ala Leu Ala Leu 1 aaa ttc cag ctg gtc aac ccc Lys Phe G n Leu Val Asn Pro 15 gtt tct ggt ctt gtg gag cag Val Ser y Leu Val G u G n ctc aaa ata gag ggt gaa aaa Leu Lys Ile G u G y G u Lys cac tcc gaa ggc cta aac ctg Hi s Ser G u G y Leu Asn Leu tgt ggt cct tcc caa ctg gaa Cys y Pro Ser G n Leu G u Page 264 Gene Sequence 24-2-06.txt at c SIle gca Al a at t SIle gtt Val acc Thr 150 aac Asn ggc Gy aag Lys gca Al a ggt Gy 230 at t SIle at c SIle ctt Leu aat Asn cgc Ar g 310 at c SIle gat Asp gca Al a gaa G u ct c Leu 120 cga Ar g ttc Phe gat Asp t cc Ser acc Thr 200 at g bet aca Thr cca Pr o gag G u ggt Gy 280 caa G n t ac Tyr ttc Phe ttg Leu gga Gy at c SIle cca Pr o at t SIle cac Hi s gca Al a 170 gaa G u gac Asp gct Al a ct c Leu at c SIle 250 gat Asp aat Asn gcc Al a ggt Gy gcc Al a 330 ggt Gy cac Hi s act Thr ct g Leu ct c Leu t ca Ser 155 gct Al a ttt Phe at c SIle gtt Val gcg Al a 235 gt c Val aat Asn gat Asp tgg Tr p t cc Ser 315 ggt Gy tt g Leu cgc Ar g gat Asp cac Hi s ccc Pr o 140 ctt Leu gaa G u gt g Val aac Asn caa G n 220 ggc Gy ttc Phe ct g Leu ttc Phe t cc Ser 300 t ac Tyr gt c Val gaa G u gt g Val gaa G u aac Asn 125 gat Asp cca Pr o cac Hi s t cc Ser acc Thr 205 ggt Gy ctt Leu cac Hi s ct g Leu cgt Ar g 285 gcg Al a at g Met ggt Gy at g bet gtc cac ggc Val His G y 95 atc gtg gaa Ile Val u 110 cct gca aac Pro Al a Asn gt c cca cac Val Pro Hi s cca gca gct Pr o Al a Al a 160 gga atc agg Gy lle Arg 175 aag cgc gtg Lys Arg Val 190 atc acc ttc Ile Thr Phe ggc cgt gcg G y Ar g Al a gtc atg ggt Val eVt G y 240 ctt tcc cgc Leu Ser Arg 255 aac aaa aag Asn Lys Lys 270 gaa ctg cgg G u Leu Ar g tac aac at t Tyr Asn Ile gt g gca ct g Val Ala Leu 320 gaa aat gcc G u Asn Al a 335 tac gga at t Tyr G y Ile gga Gy at g bet gtt Val gt a Val 145 gcg Al a cgc Ar g gt g Val cac Hi s gt a Val 225 acc Thr acc Thr tcg Ser gaa G u t ac Tyr 305 gga Gy cag G n at c SIle at c SI e gac Asp 130 gct Al a ct g Leu t at Tyr gaa G u ct g Leu 210 gat Asp cga Ar g gct Al a ggt Gy at g et 290 at a SIle cgg Ar g ttt Phe ttg Leu cgc Ar g 115 ggc Gy gt c Val t at Tyr ggt Gy at t SIle 195 ggc Gy act Thr agc Ser ggc Gy gt a Val 275 at c SI e cac Hi s gt a Val gt c Val ttc Phe 100 gat Asp at t SIe ttt Phe gcc Al a ttc Phe 180 ct g Leu aac Asn t cc Ser ggt Gy at g bet 260 aag Lys gac Asp caa G n gac Asp cgt Ar g 340 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 gag atc gat cca gag Ou Ile Asp Pro u Page 265 345 3 cgt aac gca t tg cca aac gat ggt c Ar g As n Al a Leu Pro Asn As p 03 y F 360 365 t cc aag gt g aag gt g t tt gt t at t c Ser Lys Val Lys Val Phe Val I I e F 375 380 gct agg t ac gcg gt g aag t tc gct t Al a Ar g Ty r Al a Val Lys Phe Al a 390 395 <210O> 206 <21 1> 397 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn Sequence 24-2- 06. txt 50 355 ct cga t tg at t t cc acc gat gcc ~ro Arg Leu Ilie Ser Thr As p Al a 370 ca act aat gaa gag t ta gct at c ~ro Thr Asn O31u O31u Leu Al a I Ie 385 agct ct cct ggt t aggat c cac 1219 1267 1314 <400> 206 I'bt Al a Leu Al a Leu Val O31n Gy le Ou Pr o G31y vb t ValI ValI 145 Al a Ar g ValI Hi s ValI 225 Thr ValI ValI G31y Le u O31n Le u Ar g 115 G31y ValI Tyr G31y I Ie 1 95 G31y Thr Ser Pr o O31n Ly s Le u (31u Ser Le u As p As p I Ie 1 65 Hi s (31u G31y vb t As p 245 Ou le Tyr Al a 70 li1e Al a I Ie ValI Thr 150 As n G31y Ly s Al a G31y 230 li1e Le u As n G31y Thr Ph e Thr Pr o Pr o Al a 135 G31y Ly s Thr Pr o Ser 215 vb t As p As n Ser (31u 40 Le u As p Al a (31u Le u 120 Ar g Ph e As p Ser Thr 200 vb t Thr Pr o Se r 03 y Se r 10 Al a I I e Asp Pr o As n G31y O31u Thr Pr o Leu I'b t As p Val G31y Hi s 90 Leu I I e Thr 1 Al a Pr o Leu Lys Il e Leu Phe Hi s Ser 1 Val Al a Al a 1 70 Hi s 03 u Phe 1 03 u As p I I e Al a Al a Val Pr o Leu Al a 235 03 y I I e Val 250 Ser Pr o I Ie Al a Hi s ValI (31u As n 125 As p Pr o Hi s Ser Thr 205 G31y Le u ValI Le u Hi s cys Hi s ValI Al a Pr o Al a I Ie 1 Ar g Th r Ar g vb t Phe Hi s Leu Ser Ar g 255 Page 266 Gene Sequence 24 Thr Al a Gy IVet Ser Ile Asp u Ile Asp Asn 260 265 Ser y Val Lys y Leu Ser y Val Asn Asp 275 280 Su IVet Ile Asp Asn Asn Asp n Asp Ala Trp 290 295 Tyr lie His G n Leu Arg Arg Tyr Leu G y Ser 305 310 315 Sy Arg Val Asp Thr Ile Val Phe Thr Ala y 325 330 G n Phe Val Arg G u Asp Ala Leu Ala y Leu 340 345 Su Ile Asp Pro u Arg Asn Ala Leu Pro Asn 355 360 Ile Ser Thr Asp Ala Ser Lys Val Lys Val Phe 370 375 G u G u Leu Ala lle Al a Arg Tyr Ala Val Lys 385 390 395 <210> 207 <211> 927 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(904) <223> RXA00686 <400> 207 ataggcttga acaatacgtc gttacactgg ccgatttgat ttcatcggag tgccagggga acttagagga gcattaaata -2-06. txt Leu Leu Asn 270 Phe Arg G u 285 Ser Al a Tyr 300 Tyr eVt Val Val y u G u et Tyr 350 Asp Gy Pro 365 Val Ile Pro 380 Phe Al a Lys Leu Asn Al a Asn 335 Gy Ar g Thr Lys Ar g SIe Leu 320 Al a SIe Leu Asn acctttcaaa acttttaccc atg gcg gga gga aat cgc Ar g gct Al a ct g Leu acc Thr ggc Gy gac Asp cct Pr o gaa G u gac Asp ggc Gy cgt Ar g gca Al a cgt Ar g acc Thr cca Pr o ct a Leu tgg Tr p ccg Pr o aca Thr at g bet cca Pr o ct c Leu gaa G u 75 tt c Phe gt c Val cgt Ar g agt Ser agc Ser ct c Leu at c SIle acc Thr cca Pr o acc Thr 45 aag Lys gcc Al a caa G n t cc Ser ctt Leu acc Thr aaa Lys caa G n gag G u [et Al a G y Gy Asn 1 aag gtg atc gcc gta ctg gga Lys Val I e Ala Val Leu y 15 ggt gtc act gaa atc gct gag Sy Val Thr u Ile Ala u cac cgt ctc gtt tct gaa tta His Arg Leu Val Ser G u Leu tct gat ggg cgc tac caa ttg Ser Asp Gy Arg Tyr G n Leu aat aca gga cgg cag tta cgc Asn Thr y Arg G n Leu Arg 80 ctc tac tca ctt act tcc gag Leu Tyr Ser Leu Thr Ser G u Page 267 Gene Sequence 24-2-06.txt act Thr cga Ar g cga Ar g gat Asp 150 t cc Ser aaa Lys at c l Ie gga Gy gag G u 230 gca Al a gca Al a cag G n t ac Tyr 120 cct Pr o cca Pr o cca Pr o at t SIle ggc Gy 200 gca Al a t at Tyr gcg Al a cga Ar g gt g Val acg Thr aac Asn gt a Val aca Thr 170 tcg Ser tcg Ser ggg Gy ccg Pr o at t l Ie 250 gt c Val aag Lys t cc Ser aaa Lys 155 at t SIle caa G n at c SIle tt g Leu at c SIle 235 cct Pr o cgc Ar g aaa Lys act Thr 140 cag G n gt g Val ggc Gy gcc Al a gt g Val 220 ctt Leu ttg Leu aaa Lys 110 cca Pr o gtt Val t at Tyr ccc Pr o gcc Al a 190 ccg Pr o ccc Pr o gcg Al a aca gaa G u tcg Ser aag Lys aag Lys 160 gt c Val aca Thr tgg Tr p gca Al a agt Ser 240 ttg gca Al a gct Al a at t SIle 145 ct g Leu ttg Leu cat Hi s cat Hi s cag G n 225 cag G n gct Al a ctt Leu cga Ar g 130 ct c Leu cca Pr o gct Al a gac Asp aca Thr 210 gct Al a aga Ar g t ca Ser ttg att Leu II e 115 gtc ggt Val G y ctt gcg Leu Al a ctc aac Leu Asn gcg cag Al a n 180 gag caa G u G n 195 gga aaa G y Lys gca aat Al a Asn att aca Ile Thr cac aaa Hi s Lys 260 Thr Leu Leu ggc gat acc G y Asp Thr taaacccgcc ctccatctgc ata <210> 208 <211> 268 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 208 IVet Ala G y G y Asn Arg G u Pro y 1 5 I Ie Ala Val Leu y Ala Phe u His 25 Thr G u Ile Ala G u Leu Ala Asp Leu 40 Leu Val Ser G u Leu Thr G u Gy y 55 Sy Arg Tyr n Leu Gy Leu Arg lie 70 Arg Thr Val Thr Ser Lys Val 10 Thr IVet Arg Pro Leu y Val Pro Pro Ser Thr Thr His Arg Leu Leu Ser Lys Lys Ser Asp Trp G u Leu Ala G n Asn Thr 75 Page 268 Sy Arg n Leu Arg Asp Thr Gene Sequence 24- 2- 06. txt Ala Arg Pro Phe lie n u Leu Tyr 90 Ser Al a Al a SIle 145 Leu Leu Hi s Hi s G n 225 G n Al a Thr Leu 115 Val Leu Leu Al a G u 195 Sy Al a SIle Hi s G u Asp Sy Phe Al a 165 Leu Ar g Leu Leu Lys 245 Asn Thr Ar g Ar g Asp 150 Ser Lys SIle Sy G u 230 Al a Al a Al a Al a Leu 135 G u Thr G n Gy Al a 215 Ar g Thr G u G n Tyr 120 Pr o Pr o Pr o SIle Sy 200 Al a Tyr Al a Ar g Leu 105 Sy Leu Tr p Lys Hi s 185 Al a Leu Leu Leu Lys 265 Val Lys Ser Lys 155 I le G n l Ie Leu SIle 235 Pr o Asp Asp SIle 125 Al a Ser Asn Phe Val 205 Val G n Asp Lys 110 Pr o Val Tyr Pr o Al a 190 Pr o Pr o Al a Thr Asp Ar g Sy Leu Asp 175 l le Val Thr Thr Leu 255 G u Ser Lys Lys 160 Val Thr Tr p Al a Ser 240 Leu <210> 209 <211> 1158 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1135) <223> RXA00246 <400> 209 ttgcaaggat tgtaatttaa ggcacatcta tgtcggtgtg gcaatttgcc aagtaatcca agcgagaagg agtgagtttt ccc Pr o acc Thr gt g Val gag G u gaa G u aag Lys gt a Val gat Asp acc Thr at t SIle acc Thr cca Pr o gct Al a gac Asp t cc Ser gt a Val gct Al a ctt Leu ggc Sy aag Lys gtt Val cca Pr o at c SIle 45 ccg Pr o gtt Val aag Lys tgc Cys gaa G u gaa aaa G u Lys 15 cca ggg Pro G y cac acc Hi s Thr cca cca Pro Pro Page 269 aaattacatg tgccagaaga atg acc act gct gca let Thr Thr Ala Ala 1 ttc ggt cat gac gtg Phe G y Hi s Asp Val cca cac cag gca ttg Pro His G n Ala Leu gac ctc cac gcc ttg Asp Leu His Ala Leu ttc gta cca gga cac Phe Val Pro G y His Gene Sequence 24-2-06.txt gaa G u aag Lys acc Thr gag G u gt g Val gaa G u 150 aaa Lys ggc Gy cgt Ar g aag Lys gaa G u 230 act Thr cgt Ar g gca Al a ct c Leu cgc Ar g 310 aat Asn gt a Val ggc Gy gaa G u ggt Gy 120 acc Thr gca Al a tct Ser ctt Leu at t SIle 200 ggt Gy gt a Val gtt Val gga Gy gt g Val 280 gga Gy ct a Leu gt g Val gaa G u at t I e tgc Cys t ac Tyr t ac Tyr at t l Ie acc Thr 170 cac Hi s gt a Val gaa G u aag Lys gag G u 250 at t SIle aac Asn cgc Ar g aag Lys gac Asp gtt Val 75 gt c Val at c SIle acc Thr gcc Al a ct g Leu 155 cgc Ar g at c SIle gat Asp ttt Phe tac Ty r 235 gca Al a gt g Val at c SIle caa G n cca Pr o 315 cgc Ar g gtt Val ggc Gy acc Thr caa G n gct Al a 140 tgt Cys ccg Pr o gca Al a at t SIle acc Thr 220 acc Thr gca Al a ttc Phe gt a Val gac Asp 300 acc Thr at g let gag G u aat Asn ggc Gy aat Asn 125 cgc Ar g gca Al a ggc Gy gt c Val gcc Al a 205 gt g Val aac Asn ttc Phe aac Asn ttc Phe 285 ttg Leu gt g Val cga Ar g ct c Leu gcg Al a agg Ar g 110 gga Gy at c SIle ggc Gy caa G n caa G n 190 gat Asp aat Asn ggt Gy ggc Gy ggt Gy 270 aag Lys gcc Al a agt Ser aac Asn gga Gy tgg Tr p 95 gaa G u t cc Ser cca Pr o gt g Val ttc Phe 175 t ac Tyr gac Asp gcg Al a ggc Gy cag G n 255 ct g Leu ggc Gy gaa G u gag G u cca Pr o 80 ct c Leu act Thr ttc Phe gac Asp act Thr 160 at g Net gca Al a aag Lys cgt Ar g gca Al a 240 gca Al a cca Pr o ct g Leu gcg Al a tgc Cys 320 gaa G u t ca Ser tgc Cys cag G n 130 gt g Val t ac Tyr at c SI e gcg Al a gaa G u 210 gaa G u ggc G y gat Asp gga Sy at c SIle 290 gat Asp ct c Leu cac gat Hi s Asp gcg tgt Al a Cys 100 aac gaa Asn u 115 tac at g Tyr lbet gac t ac Asp Tyr aag gca Lys Al a t cc ggt Ser G y 180 atg ggc lbet y 195 ctt gcc Leu Al a gat t ca Asp Ser gtg ctt Val Leu atg gct let Ala 260 gag ttc G u Phe 275 cgt gga Arg G y ttc ttt Phe Phe gat gag Asp u gt g Val ggc Sy gct Al a ct g Leu ct c Leu ct c Leu 165 gt c Val at g Net cgt Ar g ggc G y gt g Val 245 cga Ar g cca Pr o t cc Ser gca Al a gt c Val 325 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 ggc aag G y Lys atc gat ggt cgt gtg I e Asp y Arg Val Page 270 ene Sequence 24- 2- 06. t xt 330 335 gcg at t cgt t tc t aacggat tg t gt tgaaact gct Ala IlieArg Phe 345 <210O> 210 <21 1> 345 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn 340 1158 <400> 210 I'bt Thr Thr Phe G3 y Hi s Pr o Hi s O31n Asp Leu His5 Phe Val Pr o G3 y G3 u Hi s Tr p Ser Al a 31n Cys Asn 115 03 y 03 n Tyr 130 31y Val Asp 145 Val Tyr Lys Val Ilie Ser Al a Al a I'b t 1 95 Leu 03 u Leu 210 Asn (1u Asp 225 Hi s 03 y Val Leu Asp I'bt Pr o G31y 31u 275 Al a Al a Asp Val Al a Leu Al a Leu G y His As p Val cysa0y 1 00 0 u Al a I'b t Leu Tyr Leu Al a Leu 1 65 0 y Val 1 80 0 y b t Al a Ar g Ser 0 y Leu Val 245 Al a Ar g 260 Phe Pr o Pro O31n O31u Ph e Al a Al a Val Val 03 u Lys ValI Lys5 G31y 55 G y ValI cys Tyr As p 135 Al a ValI G31y ValI Hi s 215 Al a Al a Al a Ser Lys Asp Val Leu 40 As p Tr p Val G y Oy As p 0 u Ty r 1 0Gy 0y 120 Thr Ar g Al a Pr o Ser 0 u Leu 0 y 1 1I e Al a 200 0 y Al a Val O n Val Hi s 0 y Thr 265 Val Phe 280 As p Ser ValI ValI 75 ValI I Ie Thr Al a Le u 1 55 Ar g I Ie As p Ph e Tyr 235 Al a ValI I Ie Le u G31y Lys ValI G31y Thr O31n Al a 140 cys Pr o Al a I Ie Thr 220 Thr Al a Ph e ValI Pr o I Ie Pr o G u As n 0Oy As n 125 Ar g Al a 0Oy ValI Al a 205 ValI As n Ph e As n Ph e 285 Pr o Hi s Pr o G y Tr p 0Ou Ser Pr o ValI Ph e 1 Tyr As p Al a 0Oy 0On 255 Le u 0Oy O31y Thr Pr o Pr o Le u Thr Ph e As p Thr 1 vb t Al a Ar g Al a 240 Al a Pr o Le u Th r I Ie Ar g 1y Se r L e uVa1(3 OyTh r Ar go1n As p L e uAl a 1u Al a Page 271 ene Sequence 24- 2- 06. txt 290 295 300 Leu Asp Ph e Ph e Ala Ar g G31y Leu I Ie Lys Pro Thr Val Ser (31u Cys 305 310 315 320 Ser Leu As p (3u ValI Asn 3 y Val Leu Asp Ar g N/bt Ar g As n 3 y Lys 325 330 335 Ilie Asp 31y Ar g VaI Ala Ilie Ar g Phe 340 345 <210O> 211 <21 1> 723 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 CDS <222> (700) <223> RXA01571 <400> 211 aaact acct g ct gagagct t t gt aat t t ac ggt gt ggt t g cgct cgt agg cgctt t tgat tt t tcggt ag gct aact ggg t ggaggggt g cgt cgagaag aaa Ly s at t I Ie gct A a ggc G31y gt c ValI gt g ValI gt c ValI aac As n aag Ly s aac As n 1 50 ct a Le u cgc Ar g ggc G31y gcg A a gcg A a gt c ValI gga G31y aac As n 120 gt g ValI g at As p aag Ly s g ac As p t gc Cys tt c Ph e gga G31y t gc Cys g aa (31u gt c ValI aac As n gca A a t cc Ser cca Pr o cac Hi s ccg Pr o t cc Ser 75 ct c Le u aac As n gac As p gaa (31u gca A a 155 ggc G31y cgc Ar g ag c Ser ct c Le u g at As p gt t ValI aac As n ggc G31y cgt Ar g 140 cca Pr o cca Pr a gca A a g at As p 45 ac c Th r gt a ValI aac As n t gc Cys ac c Th r 125 tt c Ph e ct g Le u g aa (31u g at As p at c I Ie gt c ValI ac c Th r t cc Ser ct t Le u 110 at c I Ie ct g Le u ct g Le u gca A a 15 g at As p c ac Hi s ggc G31y aaa Ly s t gc Cys 95 cgc Ar g ac c Th r t gc Cys t gc Cys cct Pr a gt g ValI ac c Th r c ac Hi s t gg Tr p 80 ggc G31y gga G31y c aa O31n ag c Ser gca A a 1 60 gt g ValI 1 tt c Ph e gt t ValI at c I Ie g aa G31u aaa Lys5 g aa G31u aac As n ggc G31y at c I Ie 145 ggc (31y gag G31u at t I Ie cgc Ar g at c I Ie gt c ValI t gc Cys gt c ValI G31y 130 cca Pr a at c I Ie gt c ValI g at As p aac As n gca A a ggC G31y g aa G31u gga G31y 115 t ac Ty r gag G31u ac c Th r aag Lys5 at c I Ie g aa G31u G31y g ac As p c ag O31n 1 00 ac c Th r gct A a g aa O31u ac c Th r agt at c t ca Ser I Ie Ser gt a ValI at c I Ie aaa t gg Tr p gt t ValI cgc Ar g t gc Cys t ac Ty r g aa O31u ct t Le u t ac Ty r 1 Page 272 ene Sequence 24- 2- 06. t xt t cc cca at c gct cgc t gg aac gt t aaa gaa ggc gac aaa gta gca gtc Ser Pro I Ie Ala Arg Trp Asn Val Lys G31u G31y Asp Lys Val Ala Val 170 175 180 at g ggc ct C ggc ggg act cgg aca cat ggg t gt cca gat cgc t gc agc [Vb t (31y Leu (31y (31y Thr Arg Thr Hi s (31y Cys Pro Asp Arg Cys Ser 185 190 195 caa ggg t gc t gaggt tacc gt tct gtccc gt t (31n 1y Cys 200 <210> 212 <211> 200 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 212 Val Ser I Ie Ser Phe G3 u Val Lys Val I Ie Asp I Ie li1e Ar g As n G31u G3 u I I e A a G3 y Lys Val G31y As p 31u Cys (1u (1n 1 00 As n Val G31y Thr 115 G3 y G3 y Ty r A a 130 li1e Pr o a31u a31u 145 a3 y I I e Thr Thr As p Lys Val A a 1 80 Pr o As p Ar g Cys 1 95 <210O> 213 <211> 615 <212> DNA ValI li1e Ly s Tr p ValI Ar g Cys Tyr a31u Le u Tyr 1 65 ValI Ser Lys A a Leu a3 n a31u A a a31u Ser a31y A a Ser ValI 135 Ph e Pr o a31y a31y Ar g a31y 40 A a A a ValI a31y As n 120 ValI As p I Ie Le u Cys 200 Ar g li1e Hi s ValI a31y Ph e 1 As p ValI ValI A a a31y 1 Ly s 10 As p Cys Ph e a31y Cys 90 a31u ValI As n A a Ar g 1 70 a31y Ser Pr o Hi s Pr o Ser 75 Le u As n As p a31u A a 1 55 Tr p Thr Pr a A a As p Thr ValI As n Cys Thr 125 Ph e Le u ValI Thr A a As p Hi s a31y Ly s Cys Ar g Thr Cys Cys a31u 1 a31y Pr a ValI Thr Hi s Tr p a31y a31y a31n Ser A a 1 a31y Cys <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 CDS <222> (592) Page 273 Gene Sequence 24-2-06.txt <223> RXA01572 <400> 213 ctgctgtgcg caggcatcac cacctactcc ccaatcgctc gacaaagtag cagtcatggg cctcggcggg actcggacac gctggaacgt taaagaaggc atg ggt gtc cag atc 111 Met Gy Val On Ile gct Al a aag Lys tct Ser ct c Leu ct c Leu cag G n gga Gy tgt Cys gat Asp cgc Ar g 150 gcc Al a gaa G u gag G u acc Thr cca Pr o ct g Leu aac Asn aaa Lys 120 gat Asp gt c Val aag ggt Lys G y ctt gcc Leu Al a gat ttc Asp Phe att agc Ile Ser cac ggt Hi s G y agc ttc Ser Phe att ggc I e G y 105 cac ggc Hi s G y gca gcc Al a Al a att gat Ile Asp gct gag Al a G u aag gaa Lys G u ttc acc Phe Thr gca tcc Al a Ser gt c at g Val Vbet 75 ggt gcg G y Al a ggc atc G y lie ct c ggt Leu G y tac gac Tyr Asp 140 act gct Thr Al a 155 acc Thr ggc G y 30 cac Hi s cca Pr o gtt Val at c l Ie gaa G u 110 at g Net gtt Val gtt Val 15 gca Al a gcc Al a gt c Val gt c Val ggc Gy 95 acc Thr at c SIle gtt Val ct g Leu gct Al a ggt G y gac Asp ggt Gy ggc Gy cag G n gaa G u gcc Al a 1 t cc Ser cgc Ar g gaa G u aag Lys ct g Leu gga Gy gaa G u act Thr ggc G1y 145 cgt Ar g acg Thr ttc Phe t ac Tyr cca Pr o aaa Lys at g bet gt c Val 130 gac Asp t cc Ser ctt Leu gac Asp ct g Leu cca Pr o gt c Val ct c Leu 115 ggc Gy gtt Val ctg Leu gcg Al a ttc Phe agc Ser gag G u ct c Leu 100 gac Asp gtc Val cag G n tcg ttt gct gag gtt gag gcg gtt Ser Phe Ala G u Val 3 u Ala Val 160 taggtttact gaagttcaga ctt <210> 214 <211> 164 <212> PRT <213> Corynebacter i um gl ut am cum <400> 214 et y Val On Ile Ala Ala Ala Lys y Ala u Val Thr Val Leu 1 5 10 Ser Arg Ser Leu Arg Lys Ala u Leu Ala Lys u Leu y Ala Ala 25 Arg Thr Leu Ala Thr Ser Asp 3 u Asp Phe Phe Thr O u His Ala y 40 G u Phe Asp Phe Ile Leu Asn Thr Ile Ser Ala Ser lie Pro Val Asp Page 274 Ly s Le u G31y (31u Thr G31y 145 ValI Le u Pr o ValI Le u 115 G31y ValI A a Le u Ly s Thr Ph e As n Ph e Ly s Pr o Ser A a ValI 135 ValI ene Sequence 24- 2- 06. t xt Pr o Hi s O31y Val l'bt Ala Val 75 Leu Ser Phe G31y Ala Leu I Ie 90 As n I Ie O31y O31y IIe Pr o O31u 105 110 Lys Hi s G3 y Leu G3 y A a l'b t 120 125 As p Ala Ala Ty r As p Ar g Val 140 Val I Ie Asp Thr Ala Ser Phe 1 55 ValI (31y Thr I Ie ValI A a G31y (31y O31n (31u A a (31u 1 <210O> 215 <21 1> 1140 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1117) <223> RXA01758 <400> 215 ccccct t at t cagagt gat g gt ct accgga gaagt accca gat agcgcgc t cagaagt tc t t tagt gaaa gcagaaccaa gaccaatagc atcgaccaac gcc A a c aa O31n agt Ser c ac Hi s gcg A a gca A a gca A a c ag O31n gct A a gt g ValI gt t ValI at t I Ie ggt G31y g at As p t cc Ser tt g Le u cat Hi s cct Pr o g aa (31u tt t Ph e gt g ValI gaa (31u aaa Lys5 gca A a gt t ValI aac As n 75 ggt G31y cat Hi s t cc Ser t cg Ser g at As p ac c Thr gt a ValI ggc G31y t gc Cys ggt G31y ag g Ar g at t I Ie 45 cct Pr o t ct Ser aat As n ag a Ar g gca A a g aa (31u ggc G31y ggt G31y cgg Ar g t gc cys gag (31u 110 tt a Le u cgc Ar g gca A a gag (31u ac g Thr g ac As p aag Lys5 at g vb t 1 gcc A a gt g ValI gca A a at t I Ie gt t ValI t gc cys at t I Ie ccc Pr o gcg A a g aa (31u gca A a gca A a ggt G31y gct A a cct Pr o aaa Lys5 aat As n at c I Ie t cg Ser gga G31y g at As p tt t Ph e ggc (31y 115 t ac Tyr ct c Le u gct A a ggg G31y ac c Thr cgc Ar g t gt Cys 1 00 gt t ValI Page 275 Gene Sequence 24-2-06.txt t at Tyr gct Al a tgc Cys 150 ccc Pr o gct Al a cgc Ar g t ac Tyr ggc Gy 230 tcg Ser gga Gy aac Asn gaa G u gaa G u 310 gct Al a ggt Gy 120 at t l Ie ggt Sy gcg Al a cag G n tta Leu 200 gat Asp gct Al a ttg Leu gat Asp cag G n 280 act Thr at g et aaa Lys tgg Tr p gat Asp aca Thr gt c Val 170 gct Al a cgt Ar g aat Asn ttg Leu acc Thr 250 gga Gy at c SIle aaa Lys ct c Leu cgt Ar g 330 gca Al a ggc G y aca Thr 155 gcg Al a gcg Al a gag G u gat Asp at c SIle 235 ggt Gy gat Asp aca Thr ttt Phe gat Asp 315 tt c Phe cag aat at t G n Asn Il e 125 atg gac ttt Met Asp Phe 140 ttc aat gcg Phe Asn Al a gtc ttt gga Val Phe G y aaa at ggt Lys Vet G y 190 gag ct a gct G u Leu Al a 205 ct g cac cct Leu His Pro 220 ttg tct act Leu Ser Thr ctt tct att Leu Ser Il e atc acc gt t Ile Thr Val 270 ggt cac ct c G y Hi s Leu 285 gct cat ct c Al a Hi s Leu 300 caa gcc aac G n Al a Asn cgt att gtc Arg Ile Val gtt gtt Val Val cca gcg gag gct ctt G u Al a Leu Pr 0 t ac Tyr ttg Leu at c SIle 175 t at Tyr agg Ar g ggc Gy gcg Al a ggc Gy 255 tcg Ser act Thr cat Hi s gag G u gag G u cga Ar g 160 ggc G y cga Ar g caa G n cag G n t cc Ser 240 ggg G y gca Al a gga G y ggc G y gct Al a 320 AI a 130 gcc Al a ct g Leu tta Leu at c SIle ggc G y 210 tta Leu acg Thr ct a Leu caa G n gcg Al a 290 aaa Lys gca Al a at g et ct g Leu cgc Ar g 180 at c SIle aac Asn gaa G u cct Pr o at t SIle 260 at g Net gac Asp ctt Leu at t l Ie ggc G y gat Asp 165 ct a Leu gcc Al a cac Hi s ctt Leu ctt Leu 245 at c SIe at g bet acg Thr at t SIe t ca Ser 325 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1117 1140 ttg gag ccg aat tca Leu G u Pro Asn Ser 335 taatgccaac agcaagccca att <210> 216 <211> 339 <212> PRT <213> Corynebact eri um gl ut arri cum <400> 216 Iet Pro Lys Tyr Ile Al a Iet G n Val Ser u Ser Gy Ala Pro Leu 1 5 10 Page 276 ene Sequence 24- 2- 06. txt Ala Ala Asn Leu Val O31n Pro Ala Pro Leu Lys Ser Ar g O31u ValI Ar g ValI A a I Ie ValI Cys I Ie Pr o Pr o 145 As n G31y Thr Le u A a 225 Thr O31n A a Ser ValI 305 li1e Pr o 1I e A a A a Ser 03 y Lys G31y Thr I Ie As p Ar g Val Phe Cys Ar g 1 00 G31y Val Ser 115 O3 u A a Leu Pr o [Vbt G y Lys Leu Asp 1 65 Val Ar g Leu 1 80 Thr Ilie Ala 1 95 A a Asn Hi s Phe 03 u Leu O31u Pr o Leu 245 Thr Ilie Ilie 260 275 Asn Asp Thr Pr o Leu Ilie Ar g Ilie Ser 325 Ser Ser Hi s A a 70 A a A a Tyr A a Cy s 150 Pr o A a Ar g Tyr G31y 230 Ser G31y As n O31u O31u 310 G31y Thr O31u I Ie G31y A a A a 135 A a G31y I Ie G31y I Ie 215 G31y O31u ValI Ar g O31n 295 Ar g Cys Ph e G31y Tr p Pr o 1 05 G31y Pr o ValI A a Ph e 1 85 (31u Ser As p Ser G31y 265 li1e vb t Pr o A a ValI As n G31y Hi s A a G31y Thr 1 A a A a (31u As p I Ie 235 G31y As p Thr Ph e As p 315 As p Thr ValI G31y Cys O31n vb t 140 Ph e ValI Ly s O31u Le u 220 Le u Le u I Ie O31y A a 300 O31n lIe Pr o Ser As n Ar g As n 125 As p As n Ph e vb t Le u 205 Hi s Ser Ser Thr Hi s 285 Hi s A a O31y O31y Ar g Cys O31u 110 I Ie Ph e A a O31y O31y 1 A a Pr o Thr I Ie ValI 270 Le u Le u As n Thr Hi s Tr p O31y Ar g ValI Tyr Le u I Ie 1 Tyr Ar g O31y A a O31y 255 Ser Thr Hi s O31u Ala O1y Lys Pro Phe Ar g I Ie Val Leu 03 u 335 <210O> 217 <21 1> 1641 <212> DNA Page 277 Gene Sequence 24-2-06.txt <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1618) <223> RXA02539 <400> 217 ggctgctaag cgtgcgaatg tgcgcgttgt cacaatcgtt gaccaagtgt cacctgacgc acaggtagtg ctcaggtgga ggtggcccaa aggagaccca atg act gtc tac gca let Thr Val Tyr Ala aat Asn aac Asn gag G u ggc Gy gct Al a cac Hi s gca Al a gat Asp cgt Ar g t ac Tyr 150 aac Asn gca Al a at t SIle gga Gy at t SIle at t I e gca Al a gcg Al a at t SIle acc Thr cca Pr o 120 cag G n ttc Phe cca Pr o aac Asn t at Tyr 200 gaa G u ggc Gy cct Pr o gac Asp ggc Gy gac Asp gag G u gca Al a gat Asp gag G u ct c Leu 170 at c SIle at t SIle tcg Ser tgg Tr p act Thr gag G u act Thr at g Net ggc Gy gac Asp t cc Ser 140 at c SIle gct Al a at g et at c SIle gtt Val cca Pr o gaa G u gca Al a gt c Val gag G u gca Al a 110 ttc Phe cag G n gtt Val tgg Tr p cca Pr o 190 ggc Gy aac Asn 15 ccg Pr o gtt Val ct g Leu gct Al a cac Hi s 95 gt c Val cgc Ar g at c SIle gtt Val aag Lys 175 gct Al a gat Asp t at Tyr gt a Val ttc Phe gat Asp gaa G u 80 ct g Leu cgt Ar g t ac Tyr gac Asp ggt Gy 160 ct c Leu gag G u ct c Leu 1 gaa G u gag G u tgt Cys gct Al a cgt Ar g gaa G u gag G u ttt Phe cac Hi s 145 cag G n gca Al a cag G n at c SIle aag Lys ggc Sy gag G u gca Al a gct Al a gaa G u act Thr gct Al a 130 aac Asn at c SIle ccg Pr o acc Thr cca Pr o 210 cgc Ar g cag G n gt c Val cat Hi s ct g Leu at c SIle ctt Leu 115 ggc G y act Thr at t SIle gca Al a cca Pr o 195 gag G u t ac Tyr t ac Tyr gca Al a gca Al a at c SIle gca Al a 100 gct Al a gcg Al a gtt Val cct Pr o ctt Leu 180 gca Al a ggc Sy gag G u ctt Leu cgt Ar g gcc Al a ctg Leu gtt Val gca Al a at c SIe gct Al a tgg Tr p 165 gct Al a t cc Ser gtc Val ctc aac atc gtc aac gga Leu Asn Ile Val Asn G y ctc ggc ggt gaa gca ggc gct gca ctg tcc Leu y y G u Ala y Ala Ala Leu Ser Page 278 Gene Sequence 24-2-06.txt 220 ggc Gy 230 tct aat cgg att Ser Asn Arg Ile aag att gct ttc Lys Ile Ala Phe 225 acc ggt tcc acc gag gtc Thr G y Ser Thr G u Val 240 245 ggc aag ctg atc G y Lys Leu Ile aac Asn 250 cgc gct gca tcc Arg Ala Ala Ser aag atc att cct Lys Ile Ile Pro gt c acc Val Thr 260 ctg gag ctc Leu G u Leu t ca cag gat Ser G n Asp 280 ggc ggt aag tcc cca tcc atc ttc ttc tcc gat gtt Sy y Lys Ser Pro Ser lie Phe Phe Ser Asp Val 265 270 275 gac gcc ttc gca Asp Ala Phe Al a aag gca gtt gaa Lys Ala Val G u ggc Sy 290 ttc gcg atg Phe Al a bet ttc gcc Phe Al a 295 ctc aat cag ggt Leu Asn G n G y gaa G u 300 gtt tgt acc tgt Val Cys Thr Cys tcc cgt gca ctt Ser Arg Ala Leu gtt Val 310 cat gag tcc at c His Gu Ser Ile gat gaa ttc ctc Asp G u Phe Leu gag a u 320 ctt ggc gtg aag Leu G y Val Lys gtt cag aac atc Val n Asn Ile aag Lys 330 ctg ggt aac cca Leu y Asn Pro ctt gat act gaa acc atg atg Leu Asp Thr G u Thr IVet IVet 335 340 ggt gct cag gcg tcc cag gag cag atg gac aag atc tcc tcc tac ctg Sy Ala G n Ala Ser n G u G n Iet Asp Lys lie Ser Ser Tyr Leu 345 350 355 aag atc ggc Lys I e G y 360 cca gaa gaa ggc Pro G u G u G y gct Al a 365 caa acc ctc act G n Thr Leu Thr ggt 30y 370 ggc aag gtc G y Lys Val aac aag Asn Lys 375 gtt gat ggc atg Val Asp G y IVet gag 30u 380 aac ggt tac tac Asn G y Tyr Tyr gag cca acc gtt G u Pro Thr Val ttc Phe 390 cgc ggc acc aac Arg y Thr Asn atg agg atc ttc IVet Arg Ile Phe cgc Ar g 400 gag gaa atc ttc Su G u Ile Phe 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 cca gtc ctt tct Pro Val Leu Ser gtt Val 410 gct acc ttc agc Al a Thr Phe Ser ttc gat gag gcc Phe Asp G u Al a at c cgt lie Arg 420 att gca aac i e Al a Asn gac caa aac Asp G n Asn 440 acc aac tac ggc Thr Asn Tyr G y ct c Leu 430 ggc gct ggt gtc G y Al a G y Val tgg agc cgt Trp Ser Arg 435 gct ggt cga Ala G y Arg acc att tat cgt Thr Ile Tyr Arg gca Al a 445 ggt cgc gca atc Sy Arg Ala lie cag G n 450 gtt tgg Val Trp 455 gtc aac cag t ac Val Asn G n Tyr cac Hi s 460 aac tac cca gcg Asn Tyr Pro Ala tcc gct ttc ggt Ser Al a Phe G y gga Sy 470 tac aag gag tcc Tyr Lys G u Ser atc ggc cgt gag lie y Arg u aac Asn 480 cac ctc atg atg His Leu et bet aac cac tac cag cag acc aag aac ctg Asn His Tyr n G n Thr Lys Asn Leu ttg gtc tcc tac gat cca aac Leu Val Ser Tyr Asp Pro Asn Page 279 Gene Sequence 24- 2- 06. txt 490 495 cca acc gga ctg ttc tgatctaagc gttaagtcct aga Pro Thr Gy Leu Phe 505 <210> 218 <211> 506 <212> PRT <213> Corynebacter i um gl ut ai cum 500 1641 <400> 218 let Thr Val 1 G u Lys Arg G u G y G n Cys G u Val Al a Al a Hi s Arg Ala Leu G u u lie G u Thr Leu 115 Phe Al a G y 130 Hi s Asn Thr 145 Gn lie lle Al a Pro Al a G n Thr Pro 195 lie Pro u 210 G y Al a Al a 225 G y Ser Thr Ile Ile Pro Phe Ser Asp 275 Tyr Al a 5 Tyr G u Tyr Leu Al a Ar g Al a Al a I e Leu Al a Val 100 Al a Al a Ala I e Val Ala Pro Trp 165 Leu Al a 180 Al a Ser G y Val Leu Ser G u Val 245 Val Thr 260 Val Leu Asn Pro y Thr Asn G u Sy Al a 70 Hi s Al a Asp Ar g Ty r 150 Asn Al a SIle Leu Sy 230 Sy Leu Ser Tyr Asn Thr 55 Asp Ar g G u SIle Al a 135 Hi s Phe Sy Leu Asn 215 Ser Lys G u G n I e I e 40 Al a Al a SIle Thr Pr o 120 G n Phe Pr o Asn Tyr 200 I le Asn Leu Leu Asp 280 Sy Ser Al a Tr p Al a Tr p 105 Leu G u Asn SIle Al a 185 Leu Val Ar g SIle Sy 265 Asp G u 10 Sy Pr o Asp Gy Asp 90 G u Al a Asp G u Leu 170 l e I e Asn SIle Asn 250 a y Al a Sy Lys Val Val Lys 75 Ar g Asn l Ie Ar g Pr o 155 let Val Asn Sy Sy 235 Ar g Lys Phe Ser Tr p Thr G u Thr et Sy Asp Ser 140 I le Al a let SIle Leu 220 Lys Al a Ser Al a I le Val Sy Leu Ser G u Lys Hi s 125 Ser Sy Thr Lys SIle 205 Gy I e Al a Pr o G u 285 Asn Pr o Val Leu Al a Hi s Val Ar g SIle Val Lys 175 Al a Asp G u Phe Asp 255 I le Al a Tyr Val Phe Asp G u Leu Ar g Tyr Asp Sy 160 Leu G u Leu Al a Thr 240 Lys Phe Val G u y Phe Al a Iet Phe Al a Leu Asn Gn G y u Val Cys Thr Cys Page 280 Gene Sequence 24-2-06.txt Pr o 305 Leu Thr SIle Thr SIle 385 G u Asp Gy SIle Hi s 465 Hi s Ser Ar g Val Thr Ser 355 Gy Pr o SIle Al a Tr p 435 Al a Al a bet Asp Leu Ar g 325 bet Leu Val Val Gy 405 Ar g Ar g Ar g Gy Leu 485 Asn 295 Hi s G u Ser G n Asn II e Ala G n Al a 345 lie y Pro 360 Lys Val Asp 375 Ar g G y Thr Val Leu Ser Al a Asn Asp 425 G n Asn Thr 440 Trp Val Asn 455 Tyr Lys G u Hi s Tyr G n Thr G y Leu 505 300 Asp G u Phe Leu I e Al a 315 Lys Leu 330 Ser G n G u G u G y let Asn Asp 395 Val Ala 410 Thr Asn Ile Tyr G n Tyr Ser G y 475 G n Thr 490 Phe Asn G n Al a 365 Asn Ar g Phe Sy Al a 445 Asn Sy Asn Leu 335 Asp Thr Tyr Phe Asp 415 Sy Ar g Pr o G u Leu 495 <210> 219 <211> 430 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(430) <223> RXN03061 <400> 219 ctgccaccac tggtcattgc agaggacact ctccgtgatg gccctagagc gcgaaaccgc gcaccagaag gtgggctaaa cca gt a Pro Val cac gat His Asp ggt cct atc aac ccc agc gat ggc Ile Asn Pro Ser Asp y tcc acc cag tgg atg tcc Ser Thr G n Trp IVet Ser tca tgg gct gcg aaa act t cc acc at c Ser Thr II e 15 gcg ct c t ct Ala Leu Ser 30 ccc cgc gaa Page 281 gtcttcaggt gttagtcgca gtg tct ttg acc ttc Val Ser Leu Thr Phe 1 acc gag cta gaa aac Thr G u Leu G u Asn gat gca gtt gca gct Asp Ala Val Ala Ala aga tcc gtg gta ct c Gy acc Thr at c l Ie gt c Val cgc Ar g Ser at c SIle cac Hi s t at Tyr ccc Pr o Al a gaa G u gaa G u gca Al a cgc Ar g Al a gca Al a gct Al a 75 gaa G u tac Ty r Gene Sequence 24-2-06.txt Thr Pro Arg G u Arg Ser Val Val Leu 45 acc gaa cgc gcc caa gaa ctt gca gag Thr u Arg Ala Gn u Leu Ala u aaa tcc gat gca gaa gct ctt ggt gaa Lys Ser Asp Al a G u Al a Leu G y G u 80 ttc cgt tgg ttt gcg gaa gaa gca gtg Phe Arg Trp Phe Ala G u G u Ala Val 95 100 cag tca G n Ser 110 <210> 220 <211> 110 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 220 Val Ser Leu Thr Phe Pro Val lie Asn 1 5 Thr G u Leu G u Asn His Asp Ser Thr 25 Asp Ala Val Ala Ala G y Pro Ser Trp 40 Arg Ser Val Val Leu Thr Ala lie Phe Sn G u Leu Ala G u i e lie His Leu 70 Su Ala Leu Gy u Val Ala Tyr y Ala G u G u Ala Val Arg Leu Pro y 100 105 Pr o 10 G n Al a G u G u Al a 90 Ar g Ser Tr p Al a Al a Al a 75 G u Tyr Gy Ser Thr Thr Lys Phe G n Ser Al a Pr o G u Ser Ar g Ser 110 Thr Leu Ar g Ar g Asp Tr p SIe Ser G u Al a Al a Phe <210> 221 <211> 747 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(724) <223> RXN03150 <400> 221 tttaacagag tgcgtttcaa tgcctgtagt gttccggcaa ttttgaatgt cgttacggtt acccaaggct gaattcctga gctcaccttg tacaagatca gtg gaa gcc cag ttc 115 Val G u Ala G n Phe 1 acc tct ccc ctg ctc aac aat ggg caa acc tgt ttc ctt ggt acc cga 163 Thr Ser Pro Leu Leu Asn Asn Gy n Thr Cys Phe Leu Gy Thr Arg 15 Page 282 Gene Sequence 24-2-06.txt atc ctt gct cca aaa tca cgt tac gcg gaa gta gtc gat gca ttc acc 211 Ile Leu Ala Pro Lys Ser Arg Tyr Ala G u Val Val Asp Ala Phe Thr 30 gct ttc gct ggc agc ctg cag gtt gga gtc acg tcc tcc cct gac act 259 Ala Phe Ala G y Ser Leu G n Val G y Val Thr Ser Ser Pro Asp Thr 45 cag atc gga ccg atg gcg act gcc cgg cag cgt gag cgc gtg gaa tcc 307 Gn Ile Gy Pro Iet Ala Thr Ala Arg Gn Arg u Arg Val Gu Ser 60 tac att tcc caa ggc aaa aat gct gga gcc cgc atc act gtc ggt ggc 355 Tyr le Ser Gn G y Lys Asn Ala y Ala Arg lie Thr Val G y G y 75 80 agc cgt cca cga gat ctt gac gcc gga ttc ttc gtt gag cca aca gtg 403 Ser Arg Pro Arg Asp Leu Asp Ala G y Phe Phe Val G u Pro Thr Val 95 100 ttc gcc gat gta gac aat cgc gca gcc att gcc caa gat gaa atc ttc 451 Phe Ala Asp Val Asp Asn Arg Ala Ala lle Ala n Asp G u Ile Phe 105 110 115 gga ccg gtg ccc tct gtt gtt tcc tac caa gac gat gaa cac gcc atc 499 Sy Pro Val Pro Ser Val Val Ser Tyr G n Asp Asp G u His Ala lie 120 125 130 caa cta gcc aac gat tcc gaa ttc ggt ctc ggc gga act gtc tgg acg 547 G n Leu Ala Asn Asp Ser G u Phe y Leu Gy y Thr Val Trp Thr 135 140 145 agc gat ccc gag cgc ggc gct gca ttg gcc cgc cga gtt cac aca gga 595 Ser Asp Pro G u Arg G y Ala Ala Leu Ala Arg Arg Val His Thr G y 150 155 160 165 acc att ggc atc aac cgc tat atc cct gat ccc gcc gca cca ttt gga 643 Thr lie Gy lie Asn Arg Tyr lie Pro Asp Pro Ala Ala Pro Phe y 170 175 180 ggt gtg aaa aac agt ggc ctt ggc aga gaa ctc ggc ccc gaa ggt ctt 691 Sy Val Lys Asn Ser G y Leu y Arg G u Leu y Pro G u y Leu 185 190 195 gct tcc tac caa gaa acc caa acc att tat ctc taatccaaac tgcacctata 744 Ala Ser Tyr G n u Thr G n Thr Ile Tyr Leu 200 205 tat 747 <210> 222 <211> 208 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 222 Val G u Ala G n Phe Thr Ser Pro Leu Leu Asn Asn Oy n Thr Cys 1 5 10 Phe Leu y Thr Arg Ile Leu Ala Pro Lys Ser Arg Tyr Ala G u Val 25 Val Asp Ala Phe Thr Al a Phe Ala y Ser Leu G n Val y Val Thr 40 Ser Ser Pro Asp Thr G n lie y Pro IVet Ala Thr Ala Arg G n Arg Page 283 Gene Sequence 24-2-06.txt 55 G u Arg Val u Ser Tyr I e Ser n y Lys Asn Ala y Ala Arg 70 75 I e Thr Val G y y Ser Arg Pro Arg Asp Leu Asp Al a y Phe Phe 90 Val G u Pro Thr Val Phe Ala Asp Val Asp Asn Arg Ala Ala lie Ala 100 105 110 G n Asp G u Ile Phe G y Pro Val Pro Ser Val Val Ser Tyr G n Asp 115 120 125 Asp G u His Ala lie G n Leu Al a Asn Asp Ser G u Phe G y Leu G y 130 135 140 Sy Thr Val Trp Thr Ser Asp Pro G u Arg Gy Ala Ala Leu Ala Arg 145 150 155 160 Arg Val His Thr G y Thr lie G y lie Asn Arg Tyr lie Pro Asp Pro 165 170 175 Ala Ala Pro Phe G y G y Val Lys Asn Ser G y Leu G y Arg G u Leu 180 185 190 Sy Pro Gu y Leu Ala Ser Tyr n u Thr n Thr Ile Tyr Leu 195 200 205 <210> 223 <211> 881 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> <223> RXN01340 <400> 223 aaa gtg ggg gag atc atc gcc tcc gtc ttt gat acc ttt aat atc ccg 48 Lys Val y G u Ile I e Ala Ser Val Phe Asp Thr Phe Asn lie Pro 1 5 10 cag ggc ttg gtc tca atc atc acc acc act cga gat gca gag cta tcg 96 G n y Leu Val Ser Ile lie Thr Thr Thr Arg Asp Ala G u Leu Ser 25 gca gaa ctc atg gct gat cct cgc ttg gct aaa gtc acc ttc act gga 144 Al a G u Leu IVet Al a Asp Pro Arg Leu Al a Lys Val Thr Phe Thr G y 40 tca acc aac gtg gga cgc atc ctg gtc cgc caa tcc gcg gac cga ctg 192 Ser Thr Asn Val Gy Arg Ile Leu Val Arg G n Ser Ala Asp Arg Leu 55 ctg cgc acc tcc atg gaa ctc ggc gga aat gca gct ttt gtt atc gac 240 Leu Arg Thr Ser IVet G u Leu y y Asn Ala Ala Phe Val lie Asp 70 75 gaa gcc gca gac ctc gac gaa gcc gta tcc ggt gcc atc gcc gca aaa 288 Su Ala Ala Asp Leu Asp u Ala Val Ser y Ala Ile Ala Ala Lys 90 Page 284 Gene Sequence 24-2-06.txt ct c Leu cat Hi s cag G n at c SIle 145 at c SIle at c l Ie ttc Phe gtt Val agc Ser 225 gga Gy ttt Phe gga Gy aac gcc ggc caa gta tgc atc gca gct Asn Ala Gy On Val Cys lie Ala Ala aac cgt ttc Asn Arg Phe ttg gtt Leu Val t cc Ser 115 act Thr gca Al a ggg Gy gcc Al a cct Pr o 195 caa G n aat Asn gt c Val ggc Gy gaa G u gct gcc gaa ttc Al a Al a G u Phe 120 att ggg ccg gtg lie y Pro Val 135 gtg gat gaa gcc Val Asp u Ala 150 gtc ccc gac ggc Val Pro Asp G y 165 gtc cct gca cag Val Pro Ala G n gcc acc att gcc Ala Thr Ile Ala 200 aat tcc acc gaa Asn Ser Thr G u 215 aaa gca aca cag Lys Al a Thr G n 230 atc aac aga ggc lie Asn Arg y 245 gga caa tcc ggc G y G n Ser G y tat ctc tcc gtg Tyr Leu Ser Val 280 105 acc Thr at t SIle at c l Ie t cc Ser t ca Ser 185 act Thr ttc Phe t ac Tyr gcc Al a ttc Phe 265 cgt Ar g 110 t ca Ser tct Ser acc Thr ggc Gy 170 cgg Ar g ttc Phe ggc Gy at g et at c l Ie 250 ggc Gy t ac Tyr aag Lys gcc Al a gac Asp 155 ttc Phe at t SIle acc Thr ct c Leu gcg Al a 235 tct Ser aga Ar g ct c Leu ct g Leu cgc Ar g 140 ggc Gy ttc Phe gt g Val gac Asp gca Al a 220 gaa G u gac Asp gaa G u gct Al a gcg Al a 125 caa G n gcc Al a t at Tyr cat Hi s ttg Leu 205 gcc Al a cac Hi s cca Pr o ggc Gy ttg Leu 285 aca Thr cgc Ar g cgc Ar g cca Pr o gag G u 190 gcc Al a t ac Tyr ttg Leu gca Al a gga Gy 270 ccg Pr o gcc Al a gac Asp ct c Leu gcc Al a 175 gaa G u gaa Gu gga Gy gaa G u gca Al a 255 acc Thr 275 t gacacat ga gct gt ccggt gaa <210> 224 <211> 286 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 224 Lys Val Gy u Ile IIe Ala Ser Val Phe Asp Thr Phe Asn lie Pro 1 5 10 G n y Leu Val Ser Ile lie Thr Thr Thr Arg Asp Ala G u Leu Ser 25 Ala G u Leu IVet Ala Asp Pro Arg Leu Ala Lys Val Thr Phe Thr y 40 Ser Thr Asn Val G y Arg Ile Leu Val Arg G n Ser Ala Asp Arg Leu 55 Page 285 Gene Sequence 24-2-06.txt Leu G u Leu Hi s G n I le 145 Ile Ile Phe Val Ser 225 Sy Phe Sy Thr Ser Met u Leu Gy y Asn Ala Ala Phe Val lie Al a Asn Ser 115 Thr Al a Gy Al a Pr o 195 G n Asn Val Gy G u 275 Leu Gy Al a SIle Val Val 165 Val Al a Asn Lys SIle 245 Gy G u Val G u Pr o 135 G u Asp Al a l Ie Thr 215 Thr Ar g Ser Al a Cys Phe 120 Val Al a Gy G n Al a 200 G u G n Sy Sy Val 280 Val SIle 105 Thr SIle l Ie Ser Ser 185 Thr Phe Tyr Al a Phe 265 Sy Al a Lys Al a Asp 155 Phe SIle Thr Leu Al a 235 Ser Ar g Al a Asn Leu Ar g 140 Sy Phe Val Asp Al a 220 G u Asp G u l Ie Ar g Al a 125 G n Al a Tyr Hi s Leu 205 Al a Hi s Pr o Sy Al a Phe 110 Thr Ar g Ar g Pr o G u 190 Al a Tyr Leu Al a Sy 270 Al a Leu Al a Asp Leu Al a 175 G u G u Sy G u Al a 255 Thr G u Tyr Leu Ser Arg Tyr Leu Ala Leu Pro 285 <210> 225 <211> 1686 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1663) <223> RXN01498 <400> 225 cagtggacaa ctacttggcg ggtcttaaat cagctgtgaa ggattctgca taagctgggc accacacgag catcagaacg cgaaacgaag gtaaaagccc atg atc aaa cgt ctt 115 IVet Ile Lys Arg Leu 1 cct tta ggt ccg ctg cct aaa gaa ctt cat cag act ctg ctt gat ctg 163 Pro Leu G y Pro Leu Pro Lys G u Leu His G n Thr Leu Leu Asp Leu 15 acc gca aat gcc caa gat gcg gcg aaa gtg gag gtt ata gcg cca ttt 211 Thr Ala Asn Ala G n Asp Ala Ala Lys Val G u Val Ile Ala Pro Phe 30 Page 286 Gene Sequence 24-2-06.txt act Thr gag G u acc Thr gt a Val ggc G y acc Thr aaa Lys gt g Val 150 act Thr gt g Val gt g Val gt c Val gat Asp 230 cgg Ar g aag Lys gct Al a t cc Ser gag Gu gct Al a gca Al a aaa Lys aat Asn cgc Ar g 120 ccc Pr o aag Lys gga Gy gct Al a ct g Leu 200 ggc G y ct c Leu at g et cct Pr o ctt Leu 280 gaa Gu ct c Leu gca Al a gaa G u cgt Ar g gca Al a t ac Tyr gcg Al a gt g Val tct Ser 170 cct Pr o at t SIle G y ttc Phe gag G u 250 at t SIle cag G n at t SIle gga Gy ctt Leu cgg Ar g 75 gag G u tcg Ser gca Al a ctt Leu gt c Val 155 gat Asp gac Asp gaa G u gat Asp act Thr 235 cgt Ar g gt g Val gcg Al a t at Ty r ttt ggt Phe y 45 tca agg Ser Arg aag aag Lys Lys ct g ct c Leu Leu gct gcc Al a Al a aac aat Asn Asn 125 ccg at c Pro Ile 140 ggg cag G y G n gct gtt Al a Val ctc gcg Leu Al a gcc ggt Al a G y 205 att gtt Ile Val 220 gga t cc G y Ser ttg gtg Leu Val gcc aag Al a Lys tgt ttt Cys Phe 285 gtc gag Val G u 300 ttt gat ggt gat gag caa gac gtc Phe Asp y Asp gca Al a at c SIle at g Net gat Asp 110 gca Al a at c SIle at c SIle ccg Pr o aca Thr 190 ct g Leu ggc G y acg Thr ggt G y gat Asp 270 t cc Ser gaa G u gcc Al a ttc Phe gac Asp 95 gag G u gga G y acg Thr acg Thr gcg Al a 175 cct Pr o ccg Pr o ggt G y gcc Al a ttc Phe 255 gca Al a aac Asn cag G n ct g Leu 80 at c SIle gt g Val aag Lys aaa Lys ccg Pr o 160 ct g Leu ttc Phe cgt Ar g gcg Al a acg Thr 240 tct Ser gat Asp tcg Ser aaa Lys aag Lys gt g Val ttg Leu ttt Phe aac Asn 145 tgg Tr p ct g Leu t cc Ser gat Asp at t l Ie 225 ggc G y gcg Al a ct g Leu ggg G y Uu aag Lys gtt Val cag G n gac Asp tta Leu 130 aca Thr aat Asn gca Al a tgc Cys ttg Leu 210 gca Al a cgg Ar g gaa G u gac Asp caa G n 290 u n Asp vai tgg Tr p cat Hi s ttg Leu gtt Val 115 aat Asn caa G n t ac Tyr gga G y ttg Leu 195 at g Net gct Al a at c SIle tta Leu aag Lys 275 ttg Leu gt g Val gat Asp gaa G u 100 gcg Al a gac Asp cag G n cct Pr o aac Asn 180 at c SIle cag G n cag G n ttg Leu ggc G y 260 gt g Val tgt Cys cac Hi s ct g Leu aca Thr at c SIe aag Lys t at Tyr tta Leu 165 gca Al a at g bet gtt Val tgt Cys ggt G y 245 gga G y gaa G u gt c Val 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 gac gtg t ac Asp Val Tyr 305 Page 287 gag gag gtg att G u G u Val lie Gene Sequence 24-2-06.txt gca Al a 310 gag G u cgg Ar g ct g Leu ccc Pr o gaa Gu 390 gaa Gu gt c Val gct Al a gt g Val cat Hi s 470 gag Gu gt g Val ttt agc Phe Ser aaa t at Lys Tyr agc acc Ser Thr 345 ggt ggc G y G y 360 gta ttg Val Leu ttc ggg Phe G y gtc gat Val Asp ggg tcg G y Ser 425 ggt atc G y lle 440 acg cct Thr Pro gcg gag Al a G u cgc tgg Arg Trp t ca gac Ser Asp 505 aag Lys gag G u 330 ttt Phe aag Lys gcg Al a ccg Pr o aag Lys 410 tcg Ser ggt Gy ct g Leu gga Gy at g et 490 acc Thr gcg Al a 315 at g bet gtt Val t ca Ser gat Asp gt g Val 395 gca Al a gaa Gu at t SIle ggt Gy at t SIle 475 tct Ser gt g Val gcg aaa gcc at g Ala Lys Ala IVet ggt tcg ttg atc Gy Ser Leu Ile 335 gat cag gct aaa Asp G n Ala Lys 350 cgc cct gat att Arg Pro Asp Ile 365 gtc cca gag ggc Val Pro G u y 380 gtg ttc atc gaa Val Phe Ile u aat ggc acg ccc Asn y Thr Pro 415 acc ggc aat ctt Thr G y Asn Leu 430 aat gat ggc tac Asn Asp y Tyr 445 ggc atg aag cag Sy et Lys G n 460 aca aaa tat gcg Thr Lys Tyr Al a atg cgt ggg ccg IVet Arg G y Pr o 495 gcc aca gcg ct a Ala Thr Ala Leu 510 tcc att ggt gcc gga ttt Ser lie y Ala y Phe 320 325 aat cac gcg cag ctg gat Asn His Ala G n Leu Asp 340 gct gcg ggc gcc acg gtg Al a Al a y Ala Thr Val 355 ggt ccc ttc ttc tat gag Sy Pro Phe Phe Tyr G u 370 acc cca ctg ctc acg gag Thr Pro Leu Leu Thr G u 385 aag gta gcc aca ctg gaa Lys Val Ala Thr Leu G u 400 405 tac ggc ctg aat gcg tcc Tyr G y Leu Asn Al a Ser 420 gtt gca ggc cag ctg gaa Val Ala y n Leu u 435 gcc gcg acg tgg gcg agc Ala Ala Thr Trp Ala Ser 450 tcg ggg ctg ggg cac cgc Ser G y Leu G y His Arg 465 gag atc cga aac atc gcg G u Ile Arg Asn Ile Ala 480 485 gcc aaa atg ccg cga aag Ala Lys IVet Pro Arg Lys 500 aag ctg ggc aaa atc ttt Lys Leu G y Lys Ile Phe 515 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1686 aaa gtt ttg ccg tagcaaaaag ccggaccctt gct Lys Val Leu Pro 520 <210> 226 <211> 521 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 226 IVet lie Lys Arg Leu Pro Leu G y Pro Leu Pro Lys G u Leu His G n 1 5 10 Thr Leu Leu Asp Leu Thr Ala Asn Ala n Asp Ala Ala Lys Val G u Page 288 Gene Sequence 24-2-06.txt Thr Leu G y Phe Val Asp Lys Lys Val Leu Phe Asn 145 Tr p Leu Ser Asp SIle 225 Gy Al a Leu Gy Tyr 305 I le Hi s Al a Pr o Al a G n Tr p Hi s Leu Val 115 Asn G n Tyr Gy Leu 195 bet Al a SIle Leu Lys 275 Leu G u Al a G n Al a 355 Phe Phe Val Hi s Leu Thr SIle Lys Tyr Leu 165 Al a let Val Cys Gy 245 Gy G u Val SIle Phe 325 Asp Val G u Thr G u Thr 70 Val Gy Thr Lys Val 150 Thr Val Val Val Asp 230 Ar g Lys Al a Ser Al a 310 G u Ar g Leu Pr o Gy Hi s Thr Leu Lys Thr Ar g 135 Pr o Leu Val Hi s Thr 215 Phe Thr Asn G u Thr 295 Ar g Tr p Val Cys Thr 375 G u 40 Al a Al a Lys Asn Ar g 120 Pr o Lys Gy Al a Leu 200 Gy Leu vet Pr o Leu 280 G u Phe Lys Ser Gy 360 Val Phe Val Asn Ar g 105 Phe Gy Gy Val Lys 185 Leu Pr o et Gy Leu 265 Pr o Ar g Ser Tyr Thr 345 Gy Leu Al a G u Ar g 90 Al a Tyr Al a Val Ser 170 Pr o SIle Gy Phe G u 250 I le G n SIle Lys G u 330 Phe Lys Leu Ar g 75 G u Ser Al a Leu Val 155 Asp Asp G u Asp Thr 235 Ar g Val Al a Tyr Al a 315 let Val Ser Ser Lys Leu Al a Asn Pr o 140 Gy Al a Leu Al a SIle 220 Gy Leu Al a Cys Val 300 Al a Gy Asp Ar g Gy Ar g Lys Leu Al a Asn 125 I le G n Val Al a Gy 205 Val Ser Val Lys Phe 285 G u Lys Ser G n Pr o 365 Al a SIle let Asp 110 Al a SIle SIle Pr o Thr 190 Leu Gy Thr Gy Asp 270 Ser G u Al a Leu Al a 350 Asp Phe Asp G y Al a Phe Asp G u Gy Thr Thr Al a 175 Pr o Pr o Gy Al a Phe 255 Al a Asn Asp let SIle 335 Lys SIle n Leu I e Val Lys Lys Pr o 160 Leu Phe Ar g Al a Thr 240 Ser Asp Ser Val Ser 320 Asn Al a Gy Ala Asp Val Pro G u Gy Thr 380 Page 289 Pr o 385 Val Gy Al a Al a Gy 465 I le Lys Leu Leu Thr Asn G n 435 Tr p Gy Asn Pr o Lys 515 G u G u 405 Ser G u Ser Ar g Al a 485 Lys Phe Val Al a Phe Gy Ser 455 Gy G n Tyr Val Gene Sequence 24-2-06.txt Phe Gy Pro Val Val Phe lie 395 Val Asp Lys Ala Asn Gy Thr 410 G y Ser Ser G u Thr G y Asn 425 430 G y Ile G y Ile Asn Asp G y 440 445 Thr Pro Leu Gy Gy IVet Lys 460 Ala u Gy lie Thr Lys Tyr 475 Arg Trp IVet Ser IVet Arg y 490 Ser Asp Thr Val Ala Thr Ala 505 510 Leu Pro 520 G u Pr o 415 Leu Tyr G n Al a Pr o 495 Leu <210> 227 <211> 1575 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1552) <223> RXN02674 <400> 227 atcgacctct agggcagcag tgattgattt cataaaaatc acagttggtg aagtatccac aatcaacttt aggagacctt gct Al a gga G y gt a Val t at Tyr gcg Al a cac Hi s at c l Ie tcg Ser gcc Al a gcc Al a cga Ar g gag G u gcc Al a cga Ar g agc Ser aag Lys gcc Al a at c I e aaa Lys t cc Ser caa G n gcc Al a at c l Ie gaa G u ct c Leu aat Asn t cc Ser gct Al a cgt Ar g ct g Leu at c gga lie G y 15 at c aat I e Asn att gct I e Al a gag tgg G u Tr p gcg gct Al a Al a 80 atc aag I e Lys 95 Page 29C acaagtttgt aactaaaggt gtg act gca aca ttt Val Thr Ala Thr Phe 1 ggt cag tgg gtg gag Gy n Trp Val u cct tac gac gat tcc Pro Tyr Asp Asp Ser gat gtt gat gcc gcg Asp Val Asp Ala Ala gca gct acg ccc gct Ala Ala Thr Pro Ala gaa ctt ctt gaa gag G u Leu Leu G u G u gaa tcc ggc tcg acg Su Ser G y Ser Thr 100 Gene Sequence 24-2-06.txt cgt Ar g gaa G u aat Asn gt c Val 150 tcg Ser gcg Al a gag G u gga Gy at t SIle 230 gca Al a aac Asn cag G n t ca Ser gag Su 310 gca Al a ggt Sy cag G n aag Lys gct Al a 120 ccg Pr o gt g Val gct Al a gat Asp gcc Al a 200 gaa G u ttc Phe aat Asn ccg Pr o gcc Al a 280 aac Asn ttc Phe gga G y aag Lys gaa G u 360 aat Asn ttc Phe aaa Lys agt Ser gca Al a 170 cca Pr o gtt Val ggt Gy ggt Gy gga Gy 250 gtt Val gtt Val gt c Val gaa G u ctt Leu 330 aag Lys cca Pr o tt g Leu cct Pr o gaa G u cca Pr o 155 ct a Leu gtt Val cct Pr o gat Asp t ca Ser 235 cca Pr o gt g Val ggc G y at t SIle gca Al a 315 gtt Val at c SIle at t SIle gaa atc act G u Ile Thr 110 ggt cgt gt g Gy Arg Val 125 aac cgt gtg Asn Arg Val 140 tgg aat ttc Trp Asn Phe gcc gtg ggc Ala Val G y act ggt ggt Thr G y G y 190 gca ggc gt g Al a y Val 205 cac ttt gtc Hi s Phe Val 220 acc cca gt c Thr Pro Val at g aaa act IVet Lys Thr ctt gcc gac Leu Al a Asp 270 gct ttc cta Ala Phe Leu 285 gtt gat gct Val Asp Al a 300 gtg aag aac Val Lys Asn gga cct gt c G y Pro Val gag ttg gcc G u Leu Al a 350 gaa ggc cga Su G y Arg 365 ttg gca gga aac Leu Al a y Asn at c act aaa cat Hi s t ac Tyr cca Pr o aac Asn 175 gt a Val at c SIle acc Thr ggt Gy gtt Val 255 gcc Al a cac Hi s gca Al a at t SIle at t SIle 335 aaa Lys ct g Leu ggt G y cgc Ar g ct g Leu 160 gcc Al a at t SIle agc Ser cac Hi s cgt Ar g 240 gca Al a gac Asp cag G n gtt Val cca Pr o 320 aat Asn aag Lys gtt Val cga Ar g gt a Val 145 aac Asn gt a Val cct Pr o acg Thr gcc Al a 225 cgt Ar g ct a Leu at c SIle gga G y cat Hi s 305 acc Thr gac Asp gaa G u cat Hi s at t SIle 130 gcc Al a ct c Leu gt g Val gca Al a gtt Val 210 gt g Val gt c Val gag G u gac Asp cag G n 290 gat Asp ggc G y agt Ser ggc G y ccg Pr o 370 SIle 115 t ct Ser aag Lys tcg Ser at t SIle cga Ar g 195 gcg Al a cca Pr o ggt Gy ct c Leu gcc Al a 275 at t SIle gaa G u gat Asp cag G n gca Al a 355 cat Hi s Thr Lys cct t cg Pro Ser ggc gtt G y Val atc cgc li e Arg 165 aag cct Lys Pro 180 atc ttt Ile Phe ggc gca G y Al a aag ct g Lys Leu gag ctg G u Leu 245 ggt ggc G y G y 260 gct gcc Al a Al a tgt atg Cys IVet ttc cta Phe Leu cca agc Pro Ser 325 ct c agt Leu Ser 340 acc gt c Thr Val gtg ttc Val Phe 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 Page 291 tct gat gtc acc tct gac atg Ser Asp Val Thr Ser Asp Vbet 375 380 cct ctc atc agc gtg ctg aag Pro Leu lie Ser Val Leu Lys 390 395 ctg gcc aat gct tcc gac ttt Leu Ala Asn Ala Ser Asp Phe 410 gat att gat cgt gca gcc cag Asp Ile Asp Arg Ala Ala On 425 gtt cac atc aat gac ctc acc Val His Ile Asn Asp Leu Thr 440 ggt ggt tca aag aac tct ggc Gy y Ser Lys Asn Ser y 455 460 atc gag gag ttc acc aca gat lie G u G u Phe Thr Thr Asp 470 475 taattgtttt tcgacgtaac ccc <210> 228 <211> 484 <212> PRT <213> Corynebacter i um gl utar Gene Sequence 24-2-06.txt gaa atc gct cgt gag gaa atc G u Ile Ala Arg G u G u lie 385 gcc gat gat gag gca cac gca Al a Asp Asp G u Ala His Ala 400 ggt ttg agc gcg gca gtg tgg Gy Leu Ser Ala Ala Val Trp 415 ttt gct ctg cag att gat tcc Phe Ala Leu G n Ile Asp Ser 430 435 gtc aac gat gaa cca cac gt g Val Asn Asp G u Pro His Val 445 450 ctc ggc cgc ttc aac ggc gat Leu G y Arg Phe Asn G y Asp 465 cga tgg atc ggc atc aag cgc Arg Trp lie Gy lle Lys Arg 480 ttc Phe gca Al a tcg Ser 420 ggc G y at g bet tgg Tr p agc Ser 1267 1315 1363 1411 1459 1507 1552 1575 <400> 228 Val Thr Ala Thr Phe Ala Gy lie Asp Ala Thr Lys His Leu IIe y 1 Gy Pr o Asp Al a G u G u Gy Ar g Val 145 Tr p Asp Asp Thr Leu Gy SIle 115 Ser Lys 5 G u Ser Al a Al a G u Thr Lys Ser Val Asn SIle G u G u Ar g Ser Ser Thr 135 Gy Ser Al a 40 Al a Ar g G u Lys Al a 120 Pr o Val Asp G u Al a Ser G u Al a 105 Ser Gy lie 10 Arg Ile Ser Ser Lys G n Lys Lys Al a Al a ll e I e Ile Val u 90 Asn Leu G u Phe Pro G y Lys G u Asn 140 Ser Pro Tr p 155 Page 292 Thr Al a G n Tyr Tr p SIle Ar g 125 Ar g Asn Asn Ser Al a Ar g Leu Thr 110 Val Val Phe I e I e G u Al a SIe Leu Hi s Tyr Pr o Asn Leu Ser I Ie Ar g Ser Val 1 65 ene Sequence 24- 2- 06. t xt Ala Pro Ala Leu Ala Val Gy Asn Ala 170 175 ValI Pr o Thr Al a 225 Ar g Le u I Ie G31y Hi s 305 Th r As p O31u Hi s O31u 385 Al a Al a I Ie Pr o As n 465 li1e I Ie Ar g 1 95 Al a Pr o G31y Le u Al a 275 li1e O31u As p O31n Al a 355 Hi s I Ie Al a Tr p Ser 435 ValI As p Ar g Pr a Ph e Al a Le u Le u 245 G31y Al a vb t Le u Ser 325 Ser ValI Ph e G31y (31u 405 Ly s vb t Ph e Al a Al a (31u G31y 230 Al a As n O31n Ser O31u 310 Al a G31y O31n Ser Pr a 390 Le u As p ValI G31y I Ie 470 Ser (31u Ser 215 Ser I Ie Al a Al a I Ie 295 Ly s (31u Le u ValI As p 375 Le u Al a I Ie Hi s G31y 455 (31u As p Al a 200 (31u Ph e As n Pr a Al a 280 As n Ph e G31y Ly s (31u 360 ValI I Ie As n As p I Ie 440 Ser (31u Thr 1 85 Gy le Thr G31y Ph e 265 Al a Ar g ValI Thr (31u 345 G31y Thr Ser Al a Ar g 425 As n Ly s Ph e ValI Pr a As p Ser 235 Pr a ValI G31y Al a 315 ValI I Ie I Ie As p Le u 395 As p Al a Le u Ser Thr 475 Thr Al a Hi s 220 Thr vb t Le u Al a ValI 300 ValI G31y (31u (31u vb t 380 Ly s Ph e O31n Thr O31y 460 As p O31y O31y 205 Ph e Pr a Ly s Al a Ph e 285 As p Ly s Pr a Le u O31y 365 O31u Al a O31y Ph e ValI 445 Le u Ar g O31y 1 ValI ValI ValI Thr As p 270 Le u Al a As n ValI Al a 350 Ar g I Ie As p Le u Al a 430 As n O31y Tr p ValI I Ie Thr O31y ValI 255 Al a Hi s Al a I Ie I Ie 335 Ly s Le u Al a As p Ser 415 Le u As p Ar g I Ie <210O> 229 <21 1> 2034 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn Page 293 Gene Sequence 24-2-06.txt <220> <221> CDS <222> (101)..(2011) <223> RXN00868 <400> 229 cgatgaccca gcgcacgcct gggttcgaga tacatggacc cttgccatat gagaacgcaa acaaggaggg ataaaatttc gggcaagcat ttgatgatcg atg gct gaa acg aag IVet Al a G u Thr Lys aga Ar g act Thr at t SIle tac Tyr gag G u cgt Ar g aac Asn ttg Leu ttg Leu gct Al a 150 cag G n gca Al a gaa G u at t l Ie aca Thr gac Asp gga G y gtt Val gcg Al a ggc G y tta Leu ct g Leu 120 cag G n cgc Ar g ttc Phe acc Thr ct c Leu 200 cgc Ar g agc Ser gat Asp gga G y caa G n gt g Val t ac Tyr ggt G y agt Ser gag G u gt g Val at t SIle 170 gcg Al a gt g Val cgt Ar g cag G n at c SIle aac Asn cct Pr o cac Hi s 75 gca Al a gcg Al a gat Asp cag G n tct Ser 155 gcg Al a gt c Val ccg Pr o cca Pr o gca Al a cgc Ar g gtt Val gag G u cag G n tct Ser gct Al a act Thr cca Pr o 140 aag Lys ttg Leu acc Thr gt g Val gag G u 220 gtt Val cgc Ar g ggc G y at g bet gtt Val gca Al a gct Al a 110 gcc Al a gat Asp ttt Phe gcg Al a gcg Al a 190 ttc Phe gct Al a gaa G u 15 acc Thr at t SIle ccg Pr o gga G y tct Ser 95 acc Thr acc Thr at c SIle gat Asp at g bet 175 ctt Leu ttg Leu ttc Phe at t SIle ggc G y t ac Tyr t at Tyr 80 gtt Val acc Thr cgc Ar g ggg G y cgg Ar g 160 cgt Ar g cca Pr o cag G n 1 ctt Leu cca Pr o cag G n t ac Tyr gca Al a gga G y aac Asn gt g Val ct g Leu 145 gt g Val gt g Val gaa G u gat Asp ggt G y ggc G y gca Al a cag G n cgc Ar g ccc Pr o cgt Ar g gcg Al a 130 acg Thr cag G n ttg Leu gat Asp cgt Ar g 210 cag G n ttc Phe aag Lys cgt Ar g cac Hi s gcg Al a 100 cca Pr o cca Pr o aat Asn ccg Pr o gat Asp 180 cag G n tgg Tr p tgg Tr p gga G y cag G n aat Asn cgc Ar g acc Thr gcg Al a gtg Val gat Asp gag G u 165 ccc Pr o gct Al a cac Hi s gcg ttg gct cgt gcg att gaa Ala Leu Ala Arg Ala lie u 225 Page 294 Gene Sequence 24-2-06.txt gt c Val 230 ttg Leu ggc G y gat Asp gcc Al a cgt Ar g 310 gat Asp cat Hi s gag G u cag G n tct Ser 390 ggc G y gct Al a gcg Al a at c SIle gac Asp 470 aac Asn aaa aac gct aag aat Lys Asn Ala Lys Asn 235 tcc gat gcg gaa acg Ser Asp Al a G u Thr 250 cca gtg ggt acc tcc Pro Val Gy Thr Ser 265 gca caa aac tta ggt Ala G n Asn Leu G y 280 cgc att gcg ggt gat Arg lie Ala y Asp 300 agc gat ttc acc acc Ser Asp Phe Thr Thr 315 acc ttc atc aac atc Thr Phe Ile Asn Ile 330 act cag ttg cct gtg Thr G n Leu Pro Val 345 gct gaa gcc ctg cag Ala G u Ala Leu G n 360 atc gcg aag gaa aag Ile Ala Lys G u Lys 380 gcc ccc tcc ggt ctt Ala Pro Ser G y Leu 395 gtg cag gcg tcg aca Val G n Ala Ser Thr 410 tcc ttg cct ggt gac Ser Leu Pro G y Asp 425 ggc tac cac gtg gaa Sy Tyr His Val u 440 ggc ggt atc ggc gcg G y G y ile G y Al a 460 gac gtg gtg atc at g Asp Val Val Ile bet 475 gag ctg gtc acg gcc G u Leu Val Thr Ala 490 ccg atg atc att gct ggt ggc gga gtg Pro IVet lie lie Ala y y y Val 240 245 cag ctg cag gca ctt gtg gag cag act G n Leu G n Ala Leu Val G u G n Thr 255 260 caa gct ggt ggt ggc gtg ttg gcg tgg Sn Ala y y y Val Leu Ala Trp 270 275 ggt gtg ggt gcc acc gga acg ttg gct Sy Val y Ala Thr y Thr Leu Ala 285 290 gct gat gtg atc atc ggt atc ggt act Ala Asp Val Ile I le y I le y Thr 305 gca tct cgc act gcg ttc caa aac cct Ala Ser Arg Thr Ala Phe G n Asn Pro 320 325 aat gtt gct tcc ttc gat gct tac aag Asn Val Ala Ser Phe Asp Ala Tyr Lys 335 340 att gca gat gca cgc gag gca att gtg lie Ala Asp Ala Arg G u Ala lie Val 350 355 gga ttc acc gtg gca gag gat tac gcg Sy Phe Thr Val Ala G u Asp Tyr Ala 365 370 gct gcg tgg gac gca gaa gta gat aag Ala Ala Trp Asp Ala G u Val Asp Lys 385 gcg ctg cct gga cag ccg gag atc atc Al a Leu Pro y n Pro G u lie lie 400 405 agc gaa aaa gac gtc att gtg cag gcc Ser G u Lys Asp Val lie Val G n Ala 415 420 ctg cac aag ctg tgg cgt gtg cgc gat Leu His Lys Leu Trp Arg Val Arg Asp 430 435 tat gcg ttc tcg tgc atg ggc tat gaa Tyr Ala Phe Ser Cys IVet y Tyr G u 445 450 aag cgt gga ctt gat gcc gca ggc gat Lys Arg G y Leu Asp Al a Al a G y Asp 465 gtt ggt gat ggg tcc tac ctc atg ctc Val y Asp y Ser Tyr Leu IVet Leu 480 485 gtg gca gaa ggt atc aag gtg att gtg Val Ala G u G y Ile Lys Val lie Val 495 500 Page 295 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 Gene Sequence 24-2-06.txt gtg ctc atc caa aac cac Val Leu lie n Asn His 505 act gtc ggt tcg cag cgt Thr Val Gy Ser G n Arg 520 gag gcg aaa aac ttc cag G u Ala Lys Asn Phe G n 535 atg aat gca cgc agc tac IVet Asn Ala Arg Ser Tyr 550 555 gcg aat gcg atc gag gat Ala Asn Ala lie G u Asp 570 tcg gag aaa tcc acc ttc Ser G u Lys Ser Thr Phe 585 gca cca gac ggt gct ggt Ala Pro Asp G y Ala G y 600 act ctg gat agc acc aac Thr Leu Asp Ser Thr Asn 615 gcc ctc cag cgt ccg ctg Ala Leu G n Arg Pro Leu 630 635 <210> 230 <211> 637 <212> PRT <213> Corynebacter i um gl <400> 230 IVet Ala G u Thr Lys Arg 1 5 Leu G y His G n Trp Thr Pro Gy IVet Phe Gy Ile G n Ala Leu Lys G n Tyr Tyr G n Ala Arg Asn G u 70 Ala Arg IVet His Arg Ar g Sy Pro y Ala Thr Asn 100 Asn Arg Leu Pro Ala Leu 115 ggt G y ttt Phe ggc G y 540 ggc G y ct c Leu at c SIle tgg Tr p gcg Al a 620 ct c Leu tat gcc tcc atc ggc cac Tyr Ala Ser I I e y Hi s 510 ggt act tgg tac cgc gaa Sy Thr Trp Tyr Arg G u 525 530 gag cag att ctg cct gtt Su Gn lie Leu Pro Val 545 atg gat gtc att gaa gt g et Asp Val Ile u Val 560 aaa gca gcg atg gca acc Lys Ala Ala IVet Ala Thr 575 cac atc aac agc gat ccg His lie Asn Ser Asp Pro 590 tgg gac gtg ccg gtg tcg Trp Asp Val Pro Val Ser 605 610 gct cgt gaa gat tac ctg Ala Arg G u Asp Tyr Leu ct g Leu 515 t at Tyr gac Asp gaa G u gcg Al a ttg Leu 595 gag Gau aaa tct gaa Ser G u gac gct Asp Al a ctg gcg Leu Al a cca agc Pro Ser 565 aag gct Lys Al a 580 atc tac Ile Tyr acg t cc Thr Ser aac caa 1651 1699 1747 1795 1843 1891 1939 1987 2034 Lys Asn G n ggc G y taaaccagtt ggctaaacca aaa ut ari cum et Thr Val Val Asp G y 25 Phe G y Hi s 40 Asn Val u 55 G n Ala IVet Arg G y Thr Leu Leu Thr 105 Leu Leu Pro 120 Ser G n Al a 10 Asp lie Ar g Sy Asn Val G n Pro G u Val His G n Tyr Al a Ser 90 G y Al a Al a Ser Asp Thr Page 296 Leu G u Al a Leu Ser Al a Leu Phe 125 Val Ar g Sy et Val Al a Al a 110 Al a G u Thr SIe Pr o Sy Ser Thr Thr Val Ala Asp Pro Val Leu G n 130 135 Gene Sequence 24- 2- 06. t xt Sn Leu Ou On Pro Trp Asp Ile y Leu 145 Val Val G u Asp Al a 225 Al a Leu Sy Thr I le 305 Al a Phe Ar g Al a Al a 385 G n Val Tr p Cys Asp 465 Ser Val Ar g Thr Val 195 G u Al a Sy G u Leu 275 Thr SIle G n Al a Al a 355 Asp Val G u Val Val 435 Gy Al a Leu Asp G u 165 Pr o Al a Hi s G u Val 245 Thr Tr p Al a Thr Pr o 325 Lys Val Al a Lys SIle 405 Al a Asp G u Asp Leu 485 Al a 150 G n Al a Su I e Val 230 Leu Gy Asp Al a Ar g 310 Asp Hi s G u G n Ser 390 Sy Al a Al a SIle Asp 470 Asn Phe Leu G u bet Ar g 215 I le Tyr SIle Hi s Asn 295 Tyr Val Sy Leu Ar g 375 Phe Al a Sy Leu Al a 455 Ar g Thr Ar g Phe Thr Leu 200 Ar g Lys Ser Pr o Al a 280 Ar g Ser Thr Thr Al a 360 I le Al a Val Ser Sy 440 Sy Asp G u Pr o Ser Sy 185 Asp Pr o Asn Asp Val 265 G n SIle Asp Phe G n 345 G u Al a Pr o G n Leu 425 Tyr Sy Val Leu Val SIle 170 Al a Val Ar g Al a Al a 250 Sy Asn Al a Phe SIle 330 Leu Al a Lys Ser Al a 410 Pr o Hi s SIle Val Ser 155 Al a Val Pr o Pr o Lys 235 G u Thr Leu Sy Thr 315 Asn Pr o Leu G u Sy 395 Ser Sy Val Sy SIle 475 140 Lys Leu Thr Val G u 220 Asn Thr Ser Sy Asp 300 Thr SIle Val G n Lys 380 Leu Thr Asp G u Al a 460 bet Phe Al a SIle G u 205 Ar g Pr o G n G n Sy 285 Al a Al a Asn SIle Sy 365 Al a Al a Ser Leu Tyr 445 Lys Val Phe Al a Al a 190 Phe Al a Met Leu Al a 270 Val Asp Ser Val Al a 350 Phe Al a Leu G u Hi s 430 Al a Ar g Sy Asp vet 175 Leu Leu Al a SIe G n 255 Sy Sy Val Ar g Al a 335 Asp Thr Tr p Pr o Lys 415 Lys Phe Sy Asp Val Thr Ala Val Ala G u y Page 297 Ile Lys Val Ile Val Val Leu 500 G y Hi s Leu Ser G u Thr Val 515 Arg G u Tyr Asp Ala G u Ala 530 535 Pro Val Asp Leu Ala IVet Asn 545 550 G u Val G u Pro Ser Al a Asn 565 Ala Thr Ala Lys Ala Ser G u 580 Asp Pro Leu Ile Tyr Ala Pro 595 Val Ser G u Thr Ser Thr Leu 610 615 Tyr Leu Lys Asn G n Al a Leu 625 630 <210> 231 <211> 1142 <212> DNA <213> Cor ynebact er i um gl ut ar <220> <221> CDS <222> (1)..(1119) <223> RXN01143 <400> 231 atc cca gtt gtc acc acc ttg lie Pro Val Val Thr Thr Leu 1 5 cac gag ctg cac atg ggt atg His u Leu His e t y 'bet gtt ggt gca ctg cag cgc agc Val y Ala Leu G n Arg Ser ttt gat gac cgc gtc acc ggt Phe Asp Asp Arg Val Thr y 55 aag atc att cac gcc gac at t Lys Ile Ile His Ala Asp Ile 70 cag gtt gag gtt cca atc gtg G n Val G u Val Pro IIe Val ctg ctg gaa acc acc aag gca Leu Leu G u Thr Thr Lys Ala 100 Gene Sequence 24-2-06.txt IIe G n Asn His G y Tyr Al a 505 510 Sy Ser G n Arg Phe y Thr 520 525 Lys Asn Phe G n Gy u G n 540 Ala Arg Ser Tyr G y IVet Asp 555 Ala lie G u Asp Leu Lys Ala 570 Lys Ser Thr Phe Ile His Ile 585 590 Asp G y Al a y Trp Trp Asp 600 605 Asp Ser Thr Asn Ala Ala Arg 620 G n Arg Pro Leu Leu G y 635 Ser Tr p SIe Val Al a 575 Asn Val G u gag G u t cc Ser t cc Ser gac Asp at c SIe gct Al a at c SIe gct Al a ggc Sy 25 ct g Leu gtt Val cct Pr o gat Asp aag Lys 105 ttg ggt act Leu G y Thr 10 atg cat ggc et His G y ctg att gct Leu Il e Al a gac acc ttc Asp Thr Phe gcc gaa at c Ala G u Il e gcc cgc gaa Al a Ar g G u 90 gca gag acc Al a G u Thr Page 298 ttc Phe act Thr at c l Ie gcg Al a ggc G y gtt Val gag G u cca Pr o gt g Val ggc G y cct Pr o aag Lys ctt Leu gac Asp 110 Gene Sequence 24-2-06.txt gag tgg gtt gac tac ctc aag G u Trp Val Asp Tyr Leu Lys ggc ctc aag gca cgt ttc ccg cgt ggc Arg Phe Pro Arg y t ac Tyr acc Thr 145 ggc Gy cgc Ar g cct Pr o gct Al a acc Thr 225 gga Gy gga Gy gac Asp acc Thr aac Asn 305 gt a Val gca Al a gat Asp 115 gag G u t cc Ser cac Hi s tgg Tr p gcc Al a 195 gac Asp gca Al a cct Pr o t ac Tyr gtt Val 275 gcg Al a cgc Ar g cca Pr o gga Gy gcc Al a 355 cca Pr o gaa G u at g bet 165 aac Asn gga Gy gac Asp gaa G u gca Al a 245 aat Asn ctt Leu gaa G u gt a Val gt g Val 325 cgc Ar g at t l Ie gat Asp 135 ggc Gy gca Al a ggt Gy aag Lys tgt Cys 215 ttc Phe gtt Val aaa Lys gag G u ct g Leu 295 at c SIle gct Al a ttc Phe gaa G u Sy 120 ct g Leu ccc Pr o gct Al a gga Gy gct Al a 200 ttc Phe ccc Pr o cgc Ar g ctt Leu gga Gy 280 cca Pr o gac Asp gga Gy ttt Phe gcc Al a 360 ct g Leu gac Asp cag G n ct g Leu 185 ggc G y cag G n at t SIle caa G n cgt Ar g 265 ctt Leu gcc Al a ttc Phe t ca Ser gat Asp 345 gt c Val Leu Lys Ala gca Al a gca Al a ttc Phe 170 ggc G y gca Al a at g Met aag Lys tgg Tr p 250 aac Asn ggc Sy at c l Ie at c SIle t cc Ser 330 ggt G y agc Ser cca Pr o at t l Ie 155 gtt Val acc Thr cct Pr o acc Thr at c SIle 235 cag G n cag G n tgt Cys caa G n gt c Val 315 aac Asn gat Asp gac Asp cag G n 140 t ac Tyr gac Asp at g bet gac Asp aac Asn 220 gca Al a acc Thr ggc Sy gtt Val aag Lys 300 ggt G y t cc Ser gaa G u at t SIle 125 ttt Phe tgc Cys ttt Phe ggc G y aag Lys 205 cag G n ct a Leu ct a Leu gag G u gcc Al a 285 gct Al a gaa G u gat Asp tct Ser gat Asp 365 gt c Val gcc Al a gaa G u t ac Tyr 190 gaa G u gaa Gu at c SIle ttc Phe t ac Tyr 270 at c l Ie cga Ar g gac Asp at c SIle gca Al a 350 gcc Al a at t SIe ggc G y aag Lys 175 gca Al a gt c Val ct c Leu aac Asn t at Tyr 255 at g et cgc Ar g gag G u gca Al a cag G n 335 gca Al a gcc Al a 384 432 480 528 576 624 672 720 768 816 864 912 960 1008 1056 1104 1142 gaa t cg G u Ser 370 acc gag gca Thr G u Ala taaggagaga cccaagatgg ct a Page 299 Gene Sequence 24-2-06.txt <210> 232 <211> 373 <212> PRT <213> Corynebacteri um gl ut am cum <400> 232 li e Pro Val 1 Hi s Val Phe Lys G n Leu G u Tyr Thr 145 Sy Ar g Pr o Al a Thr 225 Sy Sy Asp Thr Asn 305 Leu Al a Asp SIle G u G u Val 115 G u Ser Hi s Tr p Al a 195 Asp Al a Pr o Tyr Val 275 Al a Ar g Val Thr Thr Leu 5 His [et Gy lbet Leu G n Arg Ser Arg Val Thr y His Ala Asp Il e 70 Val Pro II e Val Thr Thr Lys Al a 100 Asp Tyr Leu Lys G n Pro G y Asp 135 Lys G u Val y 150 G n Net Trp Ala 165 Leu Asn Ser G y 180 Leu G y Al a Lys Sy Asp y Cys 215 Val G u G y Phe 230 Sy Ala Trp Val 245 Ser Asn Thr Lys 260 Thr Leu Ser G u G u G u Val Leu 295 Pro Val Val li e 310 let Pr o Asp 40 Asp Asp Sy Ser Sy 120 Leu Pr o Al a Sy Al a 200 Phe Pr o Ar g Leu Sy 280 Pr o Asp Al a Sy Leu Val Pr o Asp Lys 105 Leu Leu Asp G n Leu 185 Sy G n SIle G n Ar g 265 Leu Al a Phe Leu 10 let Leu Asp Al a Al a 90 Al a Lys Al a Al a Phe 170 Sy Al a let Lys Tr p 250 Asn Gy l e I e Sy Hi s SIle Thr G u Ar g G u Al a Pr o l Ie 155 Val Thr Pr o Thr SIle 235 G n G n Cys G n Val 315 Thr Sy Al a Phe I le G u Thr Ar g G n 140 Tyr Asp Net Asp Asn 220 Al a Thr Sy Val Lys 300 Sy Phe Thr l Ie Al a Sy Val G u Phe 125 Phe Cys Phe Sy Lys 205 G n Leu Leu G u Al a 285 Al a G u G u Ser Ser Asp SIe Al a I e Ar g SIe Sy Lys 175 Al a Val Leu Asn Tyr 255 let Ar g G u Al a Val Trp Pro let Val Ser Ala Oy Ser Ser Asn Ser Asp Ile G n Tyr Page 300 Gene Sequence 24-2-06.txt 325 330 335 Ala Leu Gy Leu Arg Pro Phe Phe Asp Gy Asp u Ser Ala Ala G u 340 345 350 Asp Pro Ala Asp Ile His G u Ala Val Ser Asp I e Asp Ala Ala Val 355 360 365 G u Ser Thr G u Ala 370 <210> 233 <211> 793 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(793) <223> RXN01146 <400> 233 tatttagtaa aggagccaga aagtcgtgaa tgtggcagct tctcaacagc ccactcccgc cacggttgca agccgtggtc gatccgccgc ccctgagcgg aca ggt gca Thr G y Al a gca Al a at t SIle aca Thr gca Al a gca Al a gca Al a agt Ser at c SIle gac Asp cgc Ar g 150 gtt Val ggt Gy gt g Val acc Thr tct Ser ttg Leu ct g Leu at g bet 120 cca Pr o ggc G y tcg Ser gcg Al a cac Hi s t ac Tyr cca Pr o t cc Ser ggt Sy gt g Val gca Al a gtt Val ct c Leu gt g Val gt c Val gcg Al a gga G y 75 gtt Val acc Thr acc Thr tt g Leu ct g Leu 155 gag G u ct a Leu ttg Leu cag G n gca Al a ccc Pr o gac Asp aag Lys gct Al a 140 gt g Val ctt Leu gt g Val cgc Ar g act Thr aac Asn gtt Val ttc Phe 110 aac Asn gca Al a at t SI e aac Asn 15 t at Tyr cac Hi s gga Gy ttg Leu gcc Al a 95 cag G n ttc Phe ttc Phe cct Pr o gcc Al a gac Asp gag G u cgc Ar g gtt Val at c l Ie gaa G u at g bet cac Hi s aag Lys 160 at c l Ie ct c Leu ggc Gy ggc Gy cca Pr o ggc G y gat Asp acc Thr 130 gcg Al a gt c Val ttc Phe t cc Ser ggc Gy tgc Cys gct Al a gtc Val 100 cgc Ar g cct Pr o act Thr aac Asn Page 301 gaa ttg gat ttc gtc tgg cca cca a G u Leu Asp Phe Val Trp Pro Pro L 170 cca gtt tca aca cca cat gct cgc c Pro Val Ser Thr Pro His Ala Arg C 185 1 atc ggt gag gcc aag aag ccc gtc c lie Gy u Ala Lys Lys Pro Val L 200 205 aag gct gac gca cac gaa gag ctt c Lys Ala Asp Ala His G u G u Leu P 215 220 atc cca lie Pro 230 <210> 234 <211> 231 <212> PRT <213> Corynebacter i um gl ut ari cum Sequence 24-2-06.txt .ag atc gac ctg cca ggc tac cgc ys lie Asp Leu Pro y Tyr Arg 175 180 ag atc gag cag gca gtc aag ctg 3n Ile u n Ala Val Lys Leu 90 195 tt tac gtt ggt ggt ggc gta atc .eu Tyr Val Gy Gy Gy Val lie 210 gt gcg ttc gct gag tac acc ggc ,rg Ala Phe Ala G u Tyr Thr Gy 225 <400> 234 Met Thr G y Ala Lys Al a 1 Asp Pr o G n Val Thr Thr Al a Val Leu 145 Asp Leu G n Val Tyr Al a Val SIle G n l Ie 115 Asn SIle G n Gy Val 195 5 Gy Ser Hi s SIle Asp Gy Gy Asn Gy Al a 165 Ar g Leu SIle Thr Al a Al a 70 Al a Ser SIle Asp Ar g 150 G u Pr o SIle l Ie Pr o Lys Al a Thr Asn Gy Thr SIle 135 Pr o Leu Val Gy Arg Ser 10 G y Al a Arg Hi s G y Tyr G y Pro Asp Ser 90 Leu G y 105 Pro Val G n Al a Pro Val Phe Val 170 Thr Pro 185 Al a Lys Leu G u G u Leu Asn Ala Val Val Al a Sy Val Thr Thr Leu Leu 155 Tr p Hi s Lys Leu Leu G n Al a Pr o Asp Lys Al a 140 Val Pr o Al a Pr o Pr o Val Val Thr Met Al a Hi s 125 G u Asp Pr o Ar g Val 205 Val Ar g Thr Asn Val Phe 110 Asn Al a SIle Lys G n 190 Leu Tyr Hi s Sy Leu Al a G n Phe Phe Pr o SIe 175 I e Tyr Oy Oy Oy Val lie Lys Ala Asp Ala His Ou u Leu Arg Ala Phe Page 302 ene Sequence 24- 2- 06. txt 210 215 Ala Ou Tyr Thr Gy Ilie Pro 225 230 <210O> 235 <21 1> 639 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 ODS <222> 101). (616) <223> RXN01144 <400> 235 ggt gat gaat ct gcagcaga agat cct gcc gacat tcacg gccgccgttg aatcgaccga ggcataagga gagacccaag 220 aagccgt cag cgacat tgat gt c ValI at t I Ie tt c Ph e gt t ValI ct c Le u ac c Th r ac c Th r gt c ValI cca Pr o cgc Ar g 1 50 cgc Ar g cgc Ar g gt g ValI gt c ValI aag Ly s at c I Ie cgt Ar g g ac As p 120 aag Ly s ct g Le u at c lIe t ca Ser gca A a gcc A a at c I Ie cgc Ar g c ag O31n gct A a cgc Ar g c aa O31n ct g Le u ggt G31y aag Ly s gac As p CCC Pr o 75 gca A a at c I Ie cca Pr o gca A a t cc Ser 155 t cc Ser at g vb t ac c Th r gag O31u gt g ValI at c I Ie gt c ValI g ac As p ct g Le u 140 gga G31y ct c Le u ac c Th r ac a Th r aac As n aaa Ly s ct g Le u gcc A a 110 gt g ValI g ac As p at t I Ie gt t ValI 15 cga Ar g c ac Hi s at t I Ie gt c ValI gt t ValI 95 gcg A a gt t ValI gt g ValI gca A a c ag O31n cgc Ar g ggc G31y gag (31u gt g ValI 80 aag Ly s aac As n at t I Ie at g vb t ct c Le u 1 60 at g vb t 1 g ac As p gca A a at c I Ie c ag O31n cga Ar g gt c ValI at c I Ie g aa O31u g aa O31u 145 aac As n gct aat t ct A a As n Se r gt a ValI tt c Ph e aac As n at c I Ie ct t Le u t ct Ser tt c Ph e t cc Ser 130 cca Pr a cgc Ar g g ac As p aac As n cgc Ar g ac c Th r g at As p gcg A a cgc Ar g 115 ac a Th r tt c Ph e ggt (31y gga O31y ct c Le u at c I Ie aag Ly s g aa O31u g ac As p 1 00 gcc A a ggc O31y gga O31y ccg Pr o g ac As p at c I Ie gt g ValI ac g Th r c ag O31n gag O31u ag c Ser cga Ar g ac c Th r at c I Ie aag Ly s 1 acc at g gct ccg Th r I'bt Ala Pro gcc aag at c A a Lys I I e 1 t aaacagcaa t taat ct gat t gc Page 303 21> 26 ene Sequence 24- 2- 06. txt <211> 172 <21 2> PRTF <21 3> Cor ynebact er i urn gi ut ani curn <400> 236 I'bt A a Asn Ser As p A a O31n Ar g ValI I Ie O31u O31u 145 As n As p (1y Asn Leu Ar g I Ie Thr Lys As p (1u A a As p 1 00 Ar g Ala 115 Thr 03 y Phe 03 y G31y Pr o As p li1e ValI Th r O31n O31u Ser Ar g Th r I Ie Ly s 1 65 Val Thr Ar Ser Le u ValI As n Th r As n ValI G31y 135 O31u ValI 25 Ser As p Le u A a Pr o 1 05 ValI Le u I Ie 10 Ser A a A a I Ie Ar g 90 O31n A a Ar g O31n 31y Ib t Lys Thr As p (1u Pr o Val A a I I e I e Val Pr o As p A a Leu 140 Ser 03 y 1 Ph e O31u Le u Le u vb t As p Ser 125 Le u O31n Th r Th r As n Ly s Le u A a 110 ValI As p I Ie Hi s I Ie Leu Ser Val Leu Val O31n Ar g Hi s I Ie ValI ValI A a ValI ValI A a Thr I'bt Ala Pr o A a Lys I I e 1 <210O> 237 <21 1> 897 <212> DNA <213> Cor ynebact er i urn gi ut ani curn <220> <221 CDS <222> 101). (874) <223> RXA02474 <400> 237 t gct ggt ct a t t gt ggcgac cgagggcct t t gaaggt t cg acaaact gt a t aaggcct t g aat ct tgaga at t tatt t tg aggaagcaag aggaagt gt c at g agc aaa gtt gca I'b t Ser Lys Val A a 1 at g gt t acc ggt ggt gca caa ggc atc ggt cgt gga at t tca gag aag I'b t Val Thr O31y (1y Aia(3nOy Ile 1y Ar g 1y Ile Ser O31u Lys 15 ct g gca gca gat ggt t tc gat at t gcc gt a gcc gac ct g cca caa cag Leu Aia AiaAsp (1y Phe Asp IleA a Val Aia Asp Leu Pr o (1n (1n 30 gaa gaa caa gct gca gag acc atc aag ttg att gaa gct gca ggt caa (3u (3u (31n Aia Aia (1u Thr Ilie Lys Leu Ilie (31u Aia Aia 3y O31n 45 Page 304 ene Sequence 24- 2- 06. txt aag Ly s agt Ser gt a ValI g aa O31u tt t Ph e ggC G31y at c I Ie 1 50 c ag O31n t ac Ty r gag O31u t ac Ty r gcc Al a 230 gga G31y gt a ValI at t I Ie aac As n g ac As p at t I Ie 120 at c I Ie ag c Ser gct Al a cct Pr o t cc Ser 200 t cc Ser ct g Le u gt c ValI gt t ValI gag O31u G31y aag Ly s gca Al a aac As n t ac Ty r c aa O31n 1 70 at c I Ie at c I Ie at c I Ie t cg Ser gga t ta gat gt c 03 y Leu Asp Val gca gca gag aaa Al a Al a 03 u Lys 75 at c gca caa at t Ile Al a 03 n IlIe cag at c t ac t cc O3 n Il e Tyr Ser 110 gca tcc cga aag Al a Ser Ar g Lys 125 gct gca t ca at c Al a Al a Ser Ilie 140 tcc acc acc aaa Ser Thr Thr Lys 155 gaa ct C gca ccc O31u Leu Al a Pr o gt g ggc acc gga Val G31y Thr G31y 1 aac ggc aag cca Asn G31y Lys Pr o 205 gca ttg ggc cga Al a Leu G31y Ar g 220 t tc ct g gct t ct Phe Leu Al a Ser 235 acc gat Thr Asp aag gct ct t Le u aag Ly s 95 gt g ValI tt c Ph e gct Al a tt c Ph e aag Ly s 1 75 at g vb t at c I Ie cca Pr o g aa O31u ggc G31y 80 cca Pr o aac As n g at As p gct Al a gcg Al a 1 60 ggt G31y t gg Tr p ggt G31y t ca Ser aac As n 240 Ly s ggc G31y ct t Le u gt t ValI gag O31u at c I Ie 145 gt t ValI c ac Hi s gag O31u gag O31u gt a ValI 225 t cc Ser Al a t tc Ph e ct g Le u tt t Ph e ct t Le u 130 c aa O31n cgt Ar g ac c Thr c aa O31n aac As n 210 cct Pr a aac As n aat t tc gac Asn Phe Asp gt g ValI gt c ValI 1 00 gt a ValI gt a ValI tt c Ph e ct c Le u aat As n 1 g at As p aag Ly s g at As p at c I Ie ct a Le u ac c Thr tt t Ph e aaa Ly s cca Pr a ac c Thr 1 gcc Al a gcc Al a gag O31u gt a ValI ac c Thr 245 ct t gt c gac ggc ggc at g ct c t ac aac Leu Val Asp O31y O31y I'bt Leu Tyr Asn 250 255 t aggggt t gc t t tcccgcac t ca <210O> 238 <21 1> 258 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 238 I'bt Ser Lys Val Ala I'bt Val Thr Oy Oy Ala O1n Oy Ilie OyArg 1 5 10 Oy ileSer Ou Lys L eu Aia AaAs p (1y Phe Asp Ile AlaVal Ala 25 Page 305 Asp Leu Pro Gn On Ou Ou Gene Sequence 24- 2- 06. txt Sn Ala Ala u Thr Ile Lys Leu Ile 40 G u Lys Sy Leu Val G u SIle 145 Val Hi s G u G u Val 225 Ser Tyr Al a Asn Asp G u Ser 115 Sy Sy Sy Val SIle 195 Phe G u Ty r G n Asp Leu Thr Phe Lys Pr o Thr 165 Al a Al a G u Val Thr 245 Lys Ser 70 Val G u Phe Gy SIle 150 G n Ty r G u Ty r Al a 230 Sy Al a Al a Asn G u Sy Lys 135 Leu Al a Al a Leu Ser 215 Sy G n Val l Ie Asn Asp SIle 120 I le Ser Al a Pr o Ser 200 Ser Leu Val Phe Asp Al a Leu 105 G n SIle Al a Al a Sy 185 Lys Ser Val bet Sy Al a l le G n Al a Al a Ser 155 G u Val Asn Al a Phe 235 Val Leu Al a Al a SIle Ser Al a 140 Thr Leu Sy Sy Leu 220 Leu Asp Asp G u G n Tyr Ar g 125 Ser Thr Al a Thr Lys 205 G y Al a Sy Thr Leu Lys Val Phe Al a Phe Lys 175 Met SIe Pr o G u 2et 255 <210> 239 <211> 876 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(853) <223> RXA02453 <400> 239 aaccaacaaa ggtcatctca accggcttaa gaaaattctg ccagctttct gctgattgaa t cgt gccagc t cagggcat a t ct cacct aa agt aaacacc at g aaa tca at c tt c IVet Lys Ser Ile Phe 1 att tcc ggt gcg gcg aac gga att ggc aaa gct gtg gcg ttg aaa ttt I e Ser Gy Ala Ala Asn Gy ile y Lys Ala Val Al a Leu Lys Phe 15 ctt cac gaa ggt tgg ctc gtt gga gcc tac gac ctc gcg gaa atc acc Page 306 Leu Hi s G u Trp Leu Val Gene Sequence 24-2-06.txt Sy Ala Tyr Asp Leu Al a Gu I e Thr t ac Tyr gag G u acc Thr ct g Leu gt c Val cgc Ar g t ac Tyr gca Al a 150 cgc Ar g ggc G y gaa G u tgg Tr p ct g Leu 230 gcg Al a cac Hi s tgg Tr p gat Asp gac Asp ggc Gy ccg Pr o 120 cag G n ctt Leu gt c Val aag Lys gt g Val 200 aag Lys ct c Leu ct a Leu aat Asn aaa Lys gt g Val gag G u act Thr gcc Al a cag G n gag G u gtt Val 170 aag Lys cag G n aag Lys aaa Lys ctt Leu gcc Al a gac Asp 75 gag G u ctt Leu cag G n at c SIle gcg Al a 155 tgg Tr p t ca Ser gcg Al a gt g Val tct Ser 235 cgc Ar g ct a Leu aat Asn ggg Gy ggt Gy ttg Leu gcg Al a 140 ct g Leu cct Pr o ct g Leu gt a Val cat Hi s 220 ct g Leu tgg Tr p 45 gaa G u aat Asn agc Ser gcc Al a tta Leu 125 gt g Val aat Asn ttg Leu aag Lys tgg Tr p 205 cac Hi s tcg Ser ggc tac G y Tyr gac ttt Asp Phe gcc ggc Al a G y gtc gac Val Asp 95 cgc gcc Arg Ala 110 aac at g Asn IVet tat tcg Tyr Ser ttg gag Leu G u tgg gcg Trp Al a 175 cgt ttg Arg Leu 190 gat gcg Asp Al a ggg gtg G y Val cct gat Pro Asp ct c Leu gcg Al a gt a Val aag Lys gct Al a t cc Ser gct Al a tgg Tr p 160 aaa Lys ggt G y gt g Val t ca Ser cgg Ar g 240 gtt Val cac Hi s at t SIe ctt Leu cct Pr o gcg Al a 130 aag Lys aaa Lys gat Asp cgg Ar g ccg Pr o 210 ttg Leu gcg Al a cga Ar g acc Thr gag G u gca Al a t at Tyr 115 tcg Ser ttt Phe gac Asp ttg Leu at c SIle 195 aaa Lys gat Asp at g bet cag G n gga Gy gga Gy at c SIe 100 ttg Leu gcg Al a t ac Tyr gat Asp gtg Val 180 act Thr tct Ser aag Lys tgt Cys atc gcc gga i e Al a G y 250 taaatgaatt gat t atttta ggc <210> 240 <211> 251 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 240 Iet Lys Ser lie Phe lie Ser y Ala Ala Asn Gy lie y Lys Ala 1 5 10 Page 307 Gene Sequence 24-2-06.txt Val Al a Leu Phe Leu His G u G Trp Leu Val y Ala Tyr Asp Leu Asn Thr I le Leu Hi s Ser Ser 145 Ar g Thr Val Hi s Lys 225 Val G u Ar g Thr G u Al a Tyr 115 Ser Phe Asp Leu SIle 195 Lys Asp bet Thr Ser Gy Pr o Asn Al a Val Val SIle 165 Asn Pr o Ar g Al a Ty r G u Thr 70 Leu Val Ar g Ty r Al a 150 Ar g Gy G u Tr p Leu 230 Ser Ser I le G n Asn Thr Gy 135 Gy Val Val G n Al a 215 Tyr Pr o Asp Val Al a Val 105 Gy Pr o Thr Asp Al a 185 Al a Gy vet Asn Lys Val G u 90 Thr Al a G n G u Val 170 Lys G n Lys Lys Leu Al a Asp G u Leu G n SIle Al a 155 Tr p Ser Al a Val Ser 235 Tr p G u Asn Ser Al a Leu 125 Val Asn Leu Lys Tr p 205 Hi s Ser Tyr Phe Gy Asp Al a vet Ser G u Al a 175 Leu Al a Val Asp Cys Phe Al a Arg Leu lie Ala y 245 250 <210> 241 <211> 1140 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1117) <223> RXS01758 <400> 241 cccccttatt cagagtgatg gtctaccgga gaagtaccca gaccaatagc atcgaccaac gatagcgcgc tcagaagttc tttagtgaaa gcagaaccaa atg ccc aaa tac att 115 IVet Pro Lys Tyr Ile 1 gcc atg cag gta tcc gaa tcc ggt gca ccg tta gcc gcg aat ctc gtg 163 Al a Iet n Val Ser u Ser G y Ala Pro Leu Ala Ala Asn Leu Val 15 Page 308 Gene Sequence 24- 2- 06. txt caa cct gct ccg ttg aaa tcg agg gaa gtc cgc gtg gaa atc gct gct 211 G n Pro Ala Pro Leu Lys Ser Arg G u Val Arg Val G u Ile Ala Ala 30 agt ggt gtg tgc cat gca gat att ggc acg gca gca gca tcg ggg aag 259 Ser G y Val Cys His Ala Asp I e G y Thr Al a Al a Al a Ser G y Lys 45 cac act gtt ttt cct gtt acc cct ggt cat gag att gca gga acc atc 307 His Thr Val Phe Pro Val Thr Pro G y His G u IIe Ala G y Thr lie 60 gcg gaa att ggt gaa aac gta tct cgg tgg acg gtt ggt gat cgc gtt 355 Ala G u Ile y G u Asn Val Ser Arg Trp Thr Val y Asp Arg Val 75 80 gca atc ggt tgg ttt ggt ggc aat tgc ggt gac tgc gct ttt tgt cgt 403 Al a lie G y Trp Phe G y G y Asn Cys G y Asp Cys Al a Phe Cys Arg 95 100 gca ggt gat cct gtg cat tgc aga gag cgg aag att cct ggc gtt tct 451 Ala G y Asp Pro Val His Cys Arg G u Arg Lys lie Pro G y Val Ser 105 110 115 tat gcg ggt ggt tgg gca cag aat att gtt gtt cca gcg gag gct ctt 499 Tyr Al a G y G y Trp Al a G n Asn Ile Val Val Pro Ala G u Ala Leu 120 125 130 gct gcg att cca gat ggc atg gac ttt tac gag ccc gcc ccg atg ggc 547 Ala Ala lle Pro Asp Gy Met Asp Phe Tyr u Pro Ala Pro Met y 135 140 145 tgc gca ggt gtg aca aca ttc aat gcg ttg cga aac ctg aag ctg gat 595 Cys Ala y Val Thr Thr Phe Asn Ala Leu Arg Asn Leu Lys Leu Asp 150 155 160 165 ccc ggt gcg gct gtc gcg gtc ttt gga atc ggc ggt tta gtg cgc cta 643 Pro y Ala Ala Val Al a Val Phe Gy Ile Gy y Leu Val Arg Leu 170 175 180 gct att cag ttt gct gcg aaa atg ggt tat cga acc atc acc atc gcc 691 Ala lie n Phe Ala Ala Lys IVet y Tyr Arg Thr Ile Thr Ile Ala 185 190 195 cgc ggt tta gag cgt gag gag cta gct agg caa ctt ggc gcc aac cac 739 Arg G y Leu u Arg u u Leu Ala Arg G n Leu G y Ala Asn His 200 205 210 tac atc gat agc aat gat ctg cac cct ggc cag gcg tta ttt gaa ctt 787 Tyr IIe Asp Ser Asn Asp Leu His Pro G y G n Ala Leu Phe G u Leu 215 220 225 ggc ggg gct gac ttg atc ttg tct act gcg tcc acc acg gag cct ctt 835 Sy y Ala Asp Leu Ile Leu Ser Thr Ala Ser Thr Thr G u Pro Leu 230 235 240 245 tcg gag ttg tct acc ggt ctt tct att ggc ggg cag cta acc att atc 883 Ser u Leu Ser Thr y Leu Ser lie y y n Leu Thr lie lie 250 255 260 gga gtt gat ggg gga gat atc acc gtt tcg gca gcc caa ttg atg atg 931 Sy Val Asp y y Asp Ile Thr Val Ser Ala Ala n Leu IVet IVet 265 270 275 aac cgt cag atc atc aca ggt cac ctc act gga agt gcg aat gac acg 979 Asn Arg n lie lie Thr G y His Leu Thr G y Ser Ala Asn Asp Thr 280 285 290 Page 309 gaa cag act atg aaa ttt g G u G n Thr Mbt Lys Phe A 295 3 gaa cgg atg cct ctc gat c G u Arg et Pro Leu Asp C 310 315 gct ggt aaa cca cgt ttc c Ala y Lys Pro Arg Phe A 330 taatgccaac agcaagccca att <210> 242 <211> 339 <212> PRT <213> Corynebacteri um gl u Gene Sequence 24-2-06.txt ct cat ctc cat ggc gtg aaa ccg ctt att I a His Leu His G y Val Lys Pro Leu Ile 00 305 aa gcc aac gag gct att gca cgt att tca Sn Ala Asn G u Ala lie Ala Arg lie Ser 320 325 gt att gtc ttg gag ccg aat tca rg Ile Val Leu G u Pro Asn Ser 335 1027 1075 1117 1140 it am cum <400> 242 IVet Pro Lys 1 Al a Val Al a SIle Val Cys SIle Pr o Pr o 145 Asn Gy Thr Leu Al a 225 Thr Asn SIle Ser Gy Asp Phe Gy 115 G u Pr o Lys Val Thr 195 Al a Phe G u Tyr Ile Ala IVet 5 Leu Val G n Pro Al a Al a Ser G y G y Lys Hi s Thr Thr lie Ala u 70 Arg Val Ala Ile Cys Arg Al a y 100 Val Ser Tyr Ala Ala Leu Al a Ala 135 [et y Cys Ala 150 Leu Asp Pro G y 165 Arg Leu Ala lle 180 lie Ala Arg y Asn His Tyr Ile 215 G u Leu G y G y 230 Pro Leu Ser G u 245 G n Al a Val 40 Val SIle Sy Asp Sy 120 l le Sy Al a G n Leu 200 Asp Al a Leu Val Pr o 25 Cys Phe Sy Tr p Pr o 105 Sy Pr o Val Al a Phe 185 G u Ser Asp Ser G u Lys Al a Val Asn 75 Sy Hi s Al a Sy Thr 155 Al a Al a G u Asp SIle 235 Sy Ar g l Ie Pr o Ser Asn Ar g Asn 125 Asp Asn Phe bet Leu 205 Hi s Ser Al a G u Sy Sy Ar g Cys G u 110 SIle Phe Al a Sy Sy 190 Al a Pr o Thr Pro Leu Val Arg Thr Al a Hi s G u Trp Thr Sy Asp Arg Lys Val Val Tyr G u Leu Arg 160 ie G y 175 Tyr Arg Arg G n G y G n Al a Ser 240 Sy G y 255 Thr y Leu Ser lie 250 Page 310 O31n A a Ser ValI 305 li1e Pr o Thr I Ie I Ie G31y Val1 260 Leu I'b t I'b t As n Ar g 275 As n As p Thr 03 u 03 n 295 Pr o Leu I Ie 03 u Ar g 310 Ar g I Ie Ser Ala G31y 325 Ser ene Sequence 24- 2- 06. txt Asp Gy Gy Asp lieThr ValI Se r Ala 265 270 (3 n Il e I I e Thr 03 y Hi s Leu Thr 03 y 280 285 Thr I'b t Lys Phe Ala Hi s Leu Hi s G31y 300 I'b t Pr o Leu Asp (1n AlaAs n 1u Ala 315 320 Lys Pr o Ar g Phe Ar g Ilie Val Leu 031u 330 335 <210O> 243 <21 1> 1665 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 ODS <222> (1642) <223> RXA02737 <400> 243 agcacgct gc at cagt aacg gcgacat gaa at cgaat tag acacat ct tt cat taaagaa aggat cgt ga cact accat c t tcgat ct ta t gt ggccgt t gtg agc aca aac acg 11! Val Ser Thr Asn Thr ac c Thr ct c Le u ggC G31y aac As n cgc Ar g agt Ser gcc A a tt c Ph e t cc Ser cgc Ar g tt g Le u gga G31y t gg Tr p ggt G31y ggt G31y aac As n 120 t gg Tr p gct A a cga Ar g ct g Le u aaa Lys5 cgt Ar g g aa (31u gct A a aca Thr ggC G31y aag Lys5 ccc Pr o gaa (31u 75 ac g Thr tt t Ph e gca A a aac As n cct Pr o aag Lys5 cca Pr a g ac As p g aa (31u gt t ValI ac a Thr ct g Le u ggc G31y ct c Le u t tc Ph e g aa (31u cgt Ar g ggc G31y 110 aag Lys5 cgc Ar g 15 at g vb t ccc Pr a t cg Ser aaa Lys5 g aa (31u 95 aac As n cgc Ar g g ac As p gt g ValI gcc A a tt g Le u t ac Tyr aat As n tt t Ph e at c I Ie 1 ccg Pr a at c att gt a ValI gt a ValI gt t ValI g at As p g ac As p c ag O31n t tc Ph e t at Tyr ggt G31y cgc Ar g t gg Tr p g at As p aaa Ly s 130 aaa Ly s gt c ValI ct a Le u ggc G31y gcc A a cgc Ar g 1 00 gca A a cgc Ar g Page 311 Gene Sequence 24-2-06.txt acc Thr aca Thr 150 gaa G u ct c Leu gaa G u caa G n tgg Tr p 230 at t SIle cgc Ar g at g Met at c SIle t cc Ser 310 aag Lys act Thr gt g Val gag G u gac Asp 390 ggc Gy gt c Val gca Al a t cc Ser tct Ser 200 at c SIle t cc Ser ttg Leu gt c Val gaa G u 280 gt g Val cgt Ar g ctt Leu gcg Al a ttc Phe 360 gcc Al a act Thr tgg Tr p cac Hi s cgc Ar g 170 cac Hi s ttc Phe gct Al a t ac Tyr gga G y 250 cag G n at t SIle tct Ser cag G n gaa G u 330 tgt Cys ct g Leu gt g Val t cc Ser gct Al a cag G n 155 cgc Ar g gag G u cgc Ar g ct g Leu gtt Val 235 cgt Ar g aac Asn tct Ser gcg Al a tac Ty r 315 gaa G u acc Thr cgc Ar g ttt Phe ctt Leu 395 tac tac ctg tcc att cca Tyr 140 ct g Leu gt g Val ct c Leu at c SIle cgt Ar g 220 gac Asp gct Al a cac Hi s ttc Phe aca Thr 300 gct Al a gat Asp tta Leu acc Thr aaa Lys 380 ggc Gy Tyr Leu Ser lie Pro 145 gag cgt tcc ggc at g G u Arg Ser y IVet 160 at c at c gag aag cct Ile Ile u Lys Pro 175 aac cag ctg gtc aac Asn G n Leu Val Asn 190 gac cac tat ttg ggc Asp His Tyr Leu G y 205 ttt gct aac cag ctg Phe Ala Asn G n Leu 225 cac gtc cag atc acc His Val n Ile Thr 240 ggt tac tac gac ggc G y Tyr Tyr Asp G y 255 ctg atc cag ctc ttg Leu Ile G n Leu Leu 270 gtg cca gcg cag ctg Val Pro Ala G n Leu 285 aag ccg tgc tac cca Lys Pro Cys Tyr Pro 305 gcc ggt tgg cag ggc Ala y Trp On y 320 ggc ttc aac cct gag Sy Phe Asn Pro G u 335 gag atc acg tct cgt G u Ile Thr Ser Arg 350 ggt aag cgt ctt ggt Sy Lys Arg Leu y 365 gac gca cca cac cag Asp Ala Pro His G n 385 caa aac gcc atc gtg G n Asn Ala lie Val 400 Page 312 cca gat tcc ttc Pro Asp Ser Phe gct Al a ttc Phe gca Al a aag Lys 210 ttt Phe at g bet at c SIle gct Al a cag G n 290 ttg Leu tct Ser t cc Ser cgc Ar g cgc Ar g 370 cct Pr o at t SI e gaa G u ggc G y gt c Val 195 gaa G u gag G u gct Al a ggc G y ct g Leu 275 gca Al a gat Asp gag G u acc Thr tgg Tr p 355 cgt Ar g ttc Phe cgc Ar g t cc Ser cac Hi s 180 ttc Phe aca Thr cca Pr o gaa G u gca Al a 260 gtt Val gaa G u aaa Lys tta Leu act Thr 340 gct Al a gtt Val gac Asp gt g Val acc Thr 165 aac Asn cca Pr o gtt Val ct g Leu gat Asp 245 gcc Al a gcc Al a aag Lys acc Thr gt c Val 325 gag G u ggt G y act Thr ggc G y cag G n 405 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 cct gat gaa ggt gtg ctc atc cgc t Pro Asp G u G y Val Leu lie Arg F 410 gcc atg gaa gtc cgt gac gtc aac a Ala Ibt G u Val Arg Asp Val Asn l 425 4 ttc act gaa gaa tca cct gaa gca t Phe Thr G u u Ser Pro G u Ala T 440 445 ctg tta gat gaa tcc agc ctc ttc c Leu Leu Asp G u Ser Ser Leu Phe F 455 460 agc tgg aag att ctg gat cca att c Ser Trp Lys lie Leu Asp Pro lie L 470 475 gaa cca gag gat tac cca gcg ggt a Gu Pro Gu Asp Tyr Pro Ala G y T 490 gaa atg ctt tcc cgc aac ggt cac a G u IVet Leu Ser Arg Asn G y Hi s T 505 5 gcaaaaaatg at c <210> 244 <211> 514 <212> PRT <213> Cor ynebact eri um gl ut ari cum Sequence 24-2-06. t xt tc ggt tcc aag gtt cca ggt tct 'he G y Ser Lys Val Pro G y Ser 415 420 t g gac ttc tcc tac tca gaa tcc /et Asp Phe Ser Tyr Ser G u Ser .30 435 ac gag cgc ctc att ttg gat gcg yr G u Arg Leu Ile Leu Asp Ala 450 ct acc aac gag gaa gtg gaa ct g 'ro Thr Asn G u G u Val G u Leu 465 tt gaa gca tgg gat gcc gat gga .eu G u Al a Trp Asp Al a Asp G y 480 485 Icg tgg ggt cca aag agc gct gat hr Trp y Pro Lys Ser Ala Asp 495 500 cc tgg cgc agg cca taatttaggg hr Trp Arg Arg Pro 1363 1411 1459 1507 1555 1603 1652 1665 <400> 244 Val Ser Thr Asn Thr Thr Pro Ser Ser 1 Pr o SIle SIle Val Val Val Asp Asp Pr o 145 Asp Sy Asp Tyr Asp G u Asp 115 Thr Asp 5 Ar g Thr Al a Ar g Al a Leu Al a Sy Phe Pr o Asp Ar g G u Al a G u Asp Al a 135 Al a I e Al a Leu Ser Al a Met 105 Leu Asn Cys Trp Thr 10 Al a G y Arg Lys Leu Pro Lys G u Arg Thr 90 G u Phe Al a Al a Trp Al a Hi s G n 155 Page 313 Asn Pro Leu Pro Ser G y Lys Leu Leu Pro G y Phe Asp Phe G u G u Phe Arg Val Arg y 110 Thr Leu Lys 125 Tyr Tyr Leu 140 Leu G u Ara Ar g bet Pr o Ser Lys G u Asn Ar g Ser Ser Met Ala u Ser Thr G u G u 165 Gene Sequence 24-2-06.txt Ala Trp Arg Arg Val Ile Ile u Lys 170 175 Pr o Asn Gy Leu 225 Thr Gy Leu Leu Pr o 305 Gy G u Ar g Gy G n 385 Val Lys Ser Leu G u 465 Tr p Pr o Ar g Gy Val 195 G u G u Al a Gy Leu 275 Al a Asp G u Thr Tr p 355 Ar g Phe Ar g Pr o Ser 435 Leu Val Al a Ser Asn Pr o Val Leu Asp 245 Al a Al a Lys Thr Val 325 G u Gy Thr Gy G n 405 Ser Ser Al a Leu Gy 485 Asp G u Ser Asn 215 Asn Gy Asp G u Lys 295 Al a Gy Phe Pr o SIle 375 bet Asp bet Thr Leu 455 Tr p Pr o vet Ser Ser 200 I le Ser Leu Val G u 280 Val Ar g Leu Al a Phe 360 Al a Thr G u G u G u 440 Asp Lys G u Leu Al a 185 Val Leu Asn Gy I e 265 Pr o Leu Gy Ar g Al a 345 Tyr Val Val Sy Val 425 G u G u SIle Asp Ser 505 G u Ar g Leu Val 235 Ar g Asn Ser Al a Tyr 315 G u Thr Ar g Phe Leu 395 Leu Asp Pr o Ser Asp 475 Pr o Asn Leu SIle Ar g 220 Asp Al a Hi s Phe Thr 300 Al a Asp Leu Thr Lys 380 Gy SIle Val G u Leu 460 Pr o Al a Gy Asn Asp 205 Phe Hi s Gy Leu Val 285 Lys Al a Gy G u Gy 365 Asp G n Ar g Asn Al a 445 Phe SIle Gy Hi s Leu Tyr Asn G n Tyr 255 G n Al a Cys Tr p Asn 335 Thr Ar g Pr o Al a Gy 415 Asp G u Thr G u Tr p 495 Tr p Val Leu G n SIe 240 Asp Leu G n Tyr G n 320 Pr o Ser Leu Hi s SIe 400 Ser Phe Ar g Asn Al a 480 Sy Ar g Page 314 ene Sequence 24- 2- 06. txt <210O> 245 <21 1> 1203 <212> DNA <213> COr ynebact er i urn gi ut arr cumn <220> <221 ODS <222> (1180) <223> RXA02738 <400> 245 t tgt tgt taa t cggt acaaa gggt ct taag cacat ccct t cacagt tcaa gaacaat tct t t taaggaaa at t tagt tt c act tgcct gc t ct cct tgag at g t ct cac at l'bt Ser Hi s I I g at As p gag (31u gt a ValI aag Ly s gca A a aat As n g ac As p g ac As p cca Pr o at c I Ie 1 50 t tc Ph e at c I Ie gca A a at t I Ie ggt G31y g at As p gt t ValI t gt cys cgc Ar g ac c Thr 120 gt c ValI g ac As p gt t ValI c ag O31n ct c Le u t cc Ser ac c Thr t ac Tyr c ag O31n ct g Le u t cc Ser gct A a at c I Ie t tg Le u cgc Ar g 1 70 gct A a ggc G31y ggc G31y acc Thr gac As p gct A a 75 t t c Ph e at c I Ie cag O31n aag Lys5 gct A a 155 t ac Tyr gca A a act Thr aat As n aac As n gct A a gt t ValI ac c Thr gag (31u gcc A a at c I Ie 140 gag (31u cgc Ar g aac As n act Thr ag c Ser gct A a at c I Ie gcc A a at c I Ie g ac As p 110 gag (31u gca A a at c I Ie gt c ValI c ac Hi s 1 90 ct c Le u gt t ValI tt c Ph e gag (31u ag c Ser 80 gag (31u cgt Ar g t gg Tr p cca Pr o gt t ValI 1 60 gct A a gt c ValI 1 g ac As p at t I Ie gca A a ct c Le u at c I Ie t cc Ser at c I Ie gca A a ggt G31y 145 aac As n gcg A a t cc Ser g ac As p gag (31u gca A a aag Lys5 g ac As p t cc Ser t ct Ser aag Lys5 130 t ct Ser gt c ValI tt c Ph e aag Lys5 ct c Le u g aa (31u gca A a gcc A a g ac As p aac As n gct A a 115 gt t ValI tt g Le u ac c Thr at c I Ie at c I Ie 1 t c Se 2 aa Ly at gc A gt Va gg a3 10 ga As ga As cc Pr t t Le ga a3 18 ca Hi t gat e As p c cgc r Ar g 0 g t ct s Ser g t cc t Ser t ggc a (1y t cgc I Ar g c t ac y Ty r 0 c cgc p Ar g t cgt p Ar g a gca o A a g at c u I Ie 1 0 c t ct s Ser gt g gct t cc t tc t tc gt c t cc cgc gt c gac gt t gag at c gac aag cgc Page 315 Gene Sequence 24-2-06.txt Val Ala Ser Phe Phe Val Ser Arg Val Asp Val G u Ile Asp Lys Arg 200 205 210 ctc gag gca atc gga tcc gat gag gct ttg gct ctg cgc ggc aag gca 787 Leu G u Al a lie G y Ser Asp u Ala Leu Ala Leu Arg G y Lys Al a 215 220 225 ggc gtt gcc aac gct cag cgc gct tac gct gtg tac aag gag ctt ttc 835 Sy Val Ala Asn Ala G n Arg Ala Tyr Ala Val Tyr Lys G u Leu Phe 230 235 240 245 gac gcc gcc gag ctg cct gaa ggt gcc aac act cag cgc cca ctg tgg 883 Asp Ala Ala G u Leu Pro G u y Ala Asn Thr G n Arg Pro Leu Trp 250 255 260 gca tcc acc ggc gtg aag aac cct gcg tac gct gca act ctt tac gtt 931 Ala Ser Thr y Val Lys Asn Pro Ala Tyr Ala Ala Thr Leu Tyr Val 265 270 275 tcc gag ctg gct ggt cca aac acc gtc aac acc atg cca gaa ggc acc 979 Ser G u Leu Ala y Pro Asn Thr Val Asn Thr IVet Pro G u y Thr 280 285 290 atc gac gcg gtt ctg gag cag ggc aac ctg cac ggt gac acc ctg tcc 1027 IIe Asp Ala Val Leu G u G n y Asn Leu His G y Asp Thr Leu Ser 295 300 305 aac tcc gcg gca gaa gct gac gct gtg ttc tcc cag ctt gag gct ctg 1075 Asn Ser Ala Ala u Al a Asp Ala Val Phe Ser G n Leu G u Ala Leu 310 315 320 325 ggc gtt gac ttg gca gat gtc ttc cag gtc ctg gag acc gag ggt gtg 1123 Sy Val Asp Leu Ala Asp Val Phe G n Val Leu G u Thr G u y Val 330 335 340 gac aag ttc gtt gct tct tgg agc gaa ctg ctt gag tcc atg gaa gct 1171 Asp Lys Phe Val Ala Ser Trp Ser G u Leu Leu G u Ser Iet G u Ala 345 350 355 cgc ctg aag tagaatcagc acgctgcatc agt 1203 Arg Leu Lys 360 <210> 246 <211> 360 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 246 IVet Ser Hi s I e Asp Asp Leu Al a G n Leu G y Thr Ser Thr Trp Leu 1 5 10 Asp Asp Leu Ser Arg G u Arg lie Thr Ser y Asn Leu Ser G n Val 25 Ile G u G u Lys Ser Val Val y Val Thr Thr Asn Pro Ala lle Phe 40 Ala Ala Ala IVet Ser Lys y Asp Ser Tyr Asp Ala Gn lie Ala u 55 Leu Lys Ala Ala y Al a Ser Val Asp G n Ala Val Tyr Ala IVet Ser 70 75 Ile Asp Asp Val Arg Asn Ala Cys Asp Leu Phe Thr y Ile Phe G u 90 Page 316 Ser Ser Asn 1 Tyr Asp G y Gene Sequence 24-2-06.txt Arg Val Ser Ile G u Val \sp Pro Arg SIle Al a Gy 145 Asn Al a Ser G u Leu 225 Tyr G n Al a vet Gy 305 G n G u G u Al a 115 Val Leu Thr l Ie SIle 195 Asp Gy G u Pr o Leu 275 G u Thr G u G u bet 355 Ar g Ar g Al a SIle 165 Gy Ser Ar g Al a Phe 245 Tr p Val Thr Ser Leu 325 Val Al a Asp Pr o l Ie 150 Phe SIle Val Leu Gy 230 Asp Al a Ser SIle Asn 310 Gy Asp Ar g Al a Asn 135 Thr Ser Lys Al a G u 215 Val Al a Ser G u Asp 295 Ser Val Lys Leu Thr 120 Val Asp Val G n Ser 200 Al a Al a Al a Thr Leu 280 Al a Al a Asp Phe Lys 360 105 Leu let Al a Al a Al a 185 Phe l Ie Asn G u Gy 265 Al a Val Al a Leu Val 345 1 Al a SIle Leu Ar g 170 Al a Phe Gy Al a Leu 250 Val Gy Leu G u Al a 330 Al a G n Lys Al a 155 Tyr Al a Val Ser G n 235 Pr o Lys Pr o G u Al a 315 Asp Ser Al a SIle 140 G u Ar g Asn Ser Asp 220 Ar g G u Asn Asn G n 300 Asp Val Tr p Lys 125 Pr o Sy G u Sy Ar g 205 G u Al a Sy Pr o Thr 285 Sy Al a Phe Ser G u Al a SIle Val Hi s 190 Val Al a Tyr Al a Al a 270 Val Asn Val G n G u 350 Leu Thr Ser SIle 175 Asp Asp Leu Al a Asn 255 Tyr Asn Leu Phe Val 335 Leu Tr p Pr o Val 160 Al a Val Val Al a Val 240 Thr Al a Thr Hi s Ser 320 Leu Leu <210> 247 <211> 2223 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(2200) <223> RXA02739 <400> 247 cctttgccaa atttgaacca attaacctaa gtcgtagatc tgatcatcgg atctaacgaa aacgaaccaa aactttggtc ccggtttaac ccaggaagga ttg acc acc ttg acg 115 Leu Thr Thr Leu Thr 1 Page 317 Gene Sequence 24-2-06.txt ct g Leu tgg Tr p gca Al a agc Ser gat Asp tgt Cys ttc Phe ttg Leu acc Thr at g Met 150 ggc Gy gac Asp cag G n at c SIle t ac Tyr 230 gtt Val cga Ar g cct Pr o gat Asp gct Al a gct Al a cag G n cac Hi s ctt Leu cca Pr o 120 ggc Gy gct Al a t cc Ser cag G n ct g Leu 200 gac Asp gct Al a gca Al a acc Thr ctt Leu gac Asp gaa G u ctt Leu acc Thr tct Ser at g Met cac Hi s ctt Leu cgt Ar g ttc Phe 170 ggt Sy aac Asn act Thr ggc G y gaa G u 250 at c SIle cag G n acc Thr aac Asn gca Al a aac Asn 75 tt g Leu gat Asp cct Pr o ggc Gy gag G u 155 gac Asp gt c Val ct c Leu gag G u tgg Tr p 235 gct Al a cgc Ar g gcg ct c act Ala Leu Thr aag gct gt a Lys Al a Val tgt ggc tecc Cys G y Ser 45 tac acc ttg Tyr Thr Leu tgg gca ggc Tr p Al a G y acc cag tac Thr G n Tyr gac ctg aag Asp Leu Lys 110 gag tac cgc G u Tyr Arg 125 cag ggt ctt Sn G y Leu 140 cgt ggc ct a Arg G y Leu cac cac at c His His Ile acc t ct gag Thr Ser G u 190 atc gtg ttc Ile Val Phe 205 atc gct ttc I le Al a Phe 220 cag acc att Sn Thr lle gca gt g gct Al a Val Al a gtt cgc acc Val Arg Thr 270 gta cgc aat tac Val Arg Asn Tyr 15 gac act gtt cgt Asp Thr Val Arc ggc cac cca ggc G y His Pro y tac cag cgg gtt Tyr G n Arg Val cgt gac cgc ttc Arg Asp Arg Phe 80 atc cag ctt tac Ile G n Leu Tyr 95 gct ctg cgc acc Ala Leu Arg Thr cac acc aag ggc His Thr Lys G y 13C gca tct gca gtt Ala Ser Ala Val 145 ttc gac cca acc Phe Asp Pro Thr 160 tac gtc att gct Tyr Val Ile Al a 175 gca tcc tcc at c Ala Ser Ser Ile tgg gat gac aac Trp Asp Asp Asr 21C aac gag gac gtt Asn G u Asp Val 225 gag gtt gag gct Su Val u Al 240 gag gct aag aag G u Ala Lys Lys 255 atc atc ggc ttc Ile i e G y Phe Page 318 Sccc t ct Pro Ser gt c ct c Val Leu acc gca Thr Al a atg aac IVet Asn gtt ctt Val Leu ttg ggt Leu G y 100 tgg gat Trp Asp 115 gtt gag Val G u ggt atg G y let gct gct Al a Al a tct gat Ser Asp 180 gct ggc Al a G y 195 cgc at c Arg Ile gtt gct Val Ala ggc gag G y G u gac acc Asp Thr 260 gat Asp gct Al a at g bet gta Val tct Ser gga G y t cc Ser at c SIe gcc Al a gag G u 165 ggt G y acc Thr t cc Ser cgt Ar g gac Asp 245 aag Lys cca gct cca Pro Al a Pr o 275 Gene Sequence 24-2-06.txt act Thr gag G u ttc Phe 310 cgc Ar g gca Al a cgt Ar g gat Asp gca Al a 390 gca Al a gag G u ct g Leu at t SIle ttc Phe 470 acc Thr gat Asp at c SIle at g Net 280 gca Al a at c SIle gca Al a gcc Al a ctt Leu 360 aag Lys ggc Gy t cc Ser at c SIle ttc Phe 440 ct c Leu gac Asp gct Al a cca Pr o ggt Sy 520 acc Thr acc Thr gat Asp aag Lys 330 cct Pr o gcg Al a gt c Val acc Thr aac Asn 410 acc Thr at c SIle ggt Sy at g let t ac Tyr 490 cac Hi s t cc Ser ggt Gy aag Lys gag G u 315 aag Lys gag G u ggc G y gca Al a ctt Leu 395 acc Thr gag G u cgt Ar g ggc G y cgt Ar g 475 gt c Val cag G n gt c Val gct Al a act Thr 300 gtt Val gct Al a aac Asn t ac Tyr act Thr 380 cct Pr o gt g Val acc Thr gag Gu acc Thr 460 cct Pr o tgg Tr p cct Pr o ct g Leu gt g Val 285 gag G u at c SIle gca Al a aag Lys gct Al a 365 cgt Ar g gag Gu at c SIle tgg Tr p cac Hi s 445 cgc Ar g gca Al a acc Thr gtt Val cgt Ar g 525 cac ggt gct Hi s G y Al a ctt Leu gct Al a tgg Tr p gct Al a 350 gac Asp aag Lys ct g Leu aag Lys tct Ser 430 gct Al a cca Pr o gtt Val cac Hi s gaa Gu 510 cct Pr o gga G y cac Hi s cag G n 335 ct g Leu gag G u gct Al a tgg Tr p ggc Gy 415 gct Al a at g Net t ac Tyr cgt Ar g gac Asp 495 acc Thr gca Al a ttc Phe acc Thr 320 gt c Val ttc Phe ct c Leu t cc Ser ggc Gy 400 t cc Ser gag G u gga Gy ggc G y ctt Leu 480 t cc Ser ttg Leu gat Asp ga As 30 cg Ar aa Ly ga As cc Pr ga G 38 gg G cc Pr cc Pr tc Se gg G 46 gc Al at I I gc Al gc Al t ctt a Leu 290 t cct p Pro c t cc g Ser g ttc s Phe t cgc p Arg a aca o Thr 370 g gct u Al a t tcc y Ser t tcc o Ser t tac o Tyr c at c r Ile 450 a acc y Thr a gct a Al a c ggt e y t gca a Al a g aac a Asn 530 ggc gca gct G y Al a Al a gag G u ct c Leu gat Asp ct g Leu 355 tgg Tr p gca Al a gct Al a ttc Phe ggc Gy 435 ct c Leu ttc Phe ct c Leu ct g Leu ct g Leu 515 gag G u gct Al a gca Al a gag G u 340 aac Asn gat Asp ctt Leu gac Asp ggc Gy 420 cgt Ar g aac Asn ct c Leu at g Net ggc G y 500 cgc Ar g acc Thr cac Hi s gag G u 325 tgg Tr p t cc Ser gca Al a cag G n ct c Leu 405 cct Pr o aac Asn ggc Gy at c SIe gag G u 485 gaa G u gcc Al a gcc Al a 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 cag gct tgg gct gca gca ctt gag tac aag gaa On Ala Trp Ala Ala Ala Leu u Tyr Lys u 535 540 ggc Gy 545 cct aag ggt ctt Pro Lys Gy Leu Page 319 Gene Sequence 24-2-06.txt gca ctg acc cgc cag aac gtt cct gtt Ala Leu Thr Arg G n Asn Val Pro Val 550 555 gct gct gaa ggc gtt cgc cgc ggt ggc Ala Ala G u G y Val Arg Arg Gy y 570 aag gaa acc cca gat gtg atc ctc atg Lys G u Thr Pro Asp Val lie Leu IVet 585 590 ctt gca gtt aac gct gcg aag gct ctg Leu Ala Val Asn Ala Ala Lys Ala Leu 600 605 cgc gtt gtt tcc gtt cct tgc atg gat Arg Val Val Ser Val Pro Cys IVet Asp 615 620 gag tac atc gag tcc gtt ctg cct gca G u Tyr Ile u Ser Val Leu Pro Al a 630 635 gtt gaa gct ggc atc gca atg cct tgg Val u Ala Gy Ile Ala et Pro Trp 650 ggc cgt gct gtc tcc ctt gag cac ttc G y Arg Ala Val Ser Leu G u His Phe 665 670 acc ctg ttt gag aag ttc ggc atc acc Thr Leu Phe G u Lys Phe Gy Ile Thr 680 685 gcc aag gac tcc att aac ggt taattgc( Al a Lys Asp Ser Ile Asn G y 695 700 <210> 248 <211> 700 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 248 Leu Thr Thr Leu Thr Leu Ser Pro G u 1 5 Asn Tyr Pro Ser Asp Trp Ser Asp Val Val Arg Val Leu Ala Al a Asp Ala Val 40 Pro y Thr Ala IVet Ser Leu Ala Pro 55 Arg Val IVet Asn Val Asp Pro G n Asp 70 Arg Phe Val Leu Ser Cys G y His Ser Leu Tyr Leu G y G y Phe G y Leu G u 100 105 ctg gaa ggc acc aag gag aag Leu G u y Thr Lys G u Lys 560 565 tac gtc ctg gtt gag ggt tcc Tyr Val Leu Val G u Gy Ser 575 580 ggc tcc ggc tcc gag gtt cag Sy(31 Gy Ser u Val n 595 gaa gct gag ggc gtt gca gct G u Ala G u G y Val Ala Ala 610 tgg ttc cag gag cag gac gca Trp Phe n G u n Asp Ala 625 gct gtg acc gct cgt gtg tct Ala Val Thr Ala Arg Val Ser 640 645 tac cgc ttc ttg ggc acc cag Tyr Arg Phe Leu y Thr G n 655 660 ggt gct t ct gcg gat t ac cag G y Al a Ser Al a Asp Tyr G n 675 acc gat gca gtc gtg gca gcg Thr Asp Ala Val Val Ala Ala 690 1795 1843 1891 1939 1987 2035 2083 2131 2179 2223 cct gct gt t t t t a gct Leu G n Al a 10 Asp Thr Lys Su Asn Cys Leu Al a Tyr Thr Asn Trp Ser Leu Thr 90 Iet Asp Asp Page 320 Leu Al a Gy Thr Al a G n Leu Val Asp Sy Tyr Ar g SIe Al a Gene Sequence 24-2-06.txt Ar g Lys Al a 145 Pr o SIle Ser Asp Asp 225 G u Lys Gy Al a Asp 305 Ar g Lys Asp Pr o G u 385 Gy Pr o Pr o Ser Thr Trp 115 G y Val 130 Val G y Thr Al a Al a Ser I e Al a 195 Asn Arg 210 Val Val Al a G y Lys Asp Phe Pro 275 Leu G y 290 Pro G u Ser Leu Phe Asp Arg Leu 355 Thr Trp 370 Al a Al a Ser Al a Ser Phe Tyr G y 435 II e Leu 450 Asp Ser Leu Thr Pro G y His Pro G u Tyr Arg His Thr 125 SIle Al a G u 165 Gy Thr Ser Ar g Asp 245 Lys Pr o Al a Hi s G u 325 Tr p Ser Al a G n Leu 405 Pr o Asn Gy Thr bet 150 Gy Asp G n SIle Ty r 230 Val Ar g Thr G u Phe 310 Ar g Al a Ar g Asp Al a 390 Al a G u Leu SIle Thr 135 Al a G u Leu G n G u 215 Lys Al a Pr o let Val 295 Al a Al a Al a G u G u 375 Leu Gy Ser Hi s Ser 455 120 Gy Al a Ser G n Leu 200 Asp Al a Al a Thr let 280 Al a l Ie Al a Al a Leu 360 Lys Gy Ser SIle Phe 440 Leu Pr o Ar g Pr o G u 185 Gy Asn Tyr l Ie Phe 265 Asn Al a Asp G n Asn 345 Pr o Gy Lys Asn Ser 425 Gy Hi s Gy G u 155 Asp Val Leu G u Tr p 235 Al a Ar g Gy Lys G u 315 Lys G u Gy Al a Leu 395 Thr G u Ar g Gy G n 140 Ar g Hi s Thr SIle SIle 220 G n Al a Val Al a Thr 300 Val Al a Asn Tyr Thr 380 Pr o Val Thr G u Thr 460 Sy Sy Hi s Ser Val 205 Al a Thr Val Ar g Val 285 G u SIle Al a Lys Al a 365 Ar g G u SIle Tr p Hi s 445 Ar g Leu Leu SIle G u 190 Phe Phe SIle Al a Thr 270 Hi s Leu Al a Tr p Al a 350 Asp Lys Leu Lys Ser 430 Al a Pr o Al a Phe Tyr 175 Al a Tr p Asn G u G u 255 I le Sy Sy Hi s G n 335 Leu G u Al a Tr p Sy 415 Al a let Tyr Ser Asp 160 Val Ser Asp G u Val 240 Al a SIe Al a Phe Thr 320 Val Phe Leu Ser Sy 400 Ser G u Sy Sy Sy Thr Phe Leu Ile Phe Ser Asp Tyr IVet Arg Pro Ala Val Arg Leu Page 321 Gene Sequence 24-2-06.txt 465 Al a SIle Al a Al a Gy 545 Gy Leu Gy G u G n 625 Thr Phe Ser Al a 475 Val Trp Thr His Leu Leu Leu 515 G u Lys Lys G u G u 595 Val G n Ar g Gy Asp 675 Val G u 485 G u Al a Al a Leu Lys 565 Ser G n Al a Al a Ser 645 G n G n Al a Thr Asp l Ie G n Al a 550 Al a Lys Leu Ar g G u 630 Val Sy Thr Al a Asp Al a Tyr G y Pro Thr 505 Pro G y Leu 520 Al a Trp Al a 535 Leu Thr Arg Al a G u G y G u Thr Pro 585 Al a Val Asn 600 Val Val Ser 615 Tyr Ile G u G u Al a G y Ar g Al a Val 665 Leu Phe G u 680 Lys Asp Ser 695 n Val Al a Asn 555 Ar g Val Al a Pr o Val 635 Al a Leu Phe Asn Val Ar g 525 G u Pr o Sy Leu Al a 605 let Pr o Pr o Hi s SIle 685 480 Asp Ser 495 Thr Leu Al a Asp Lys G u Leu G u 560 Tyr Val 575 G y Ser G u Al a Trp Phe Al a Val 640 Tyr Arg 655 G y Al a Thr Asp <210> 249 <211> 793 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (48)..(770) <223> RXA00965 <400> 249 agattgcggg cctcggcttc attgaaaaca agacggtgtt tgaataa atg aca act let Thr Thr 1 ttc cac gat ctt ccg ctg gag gag cgg ctg aca ctg gcc agg ttg ggc Phe His Asp Leu Pro Leu G u G u Arg Leu Thr Leu Ala Arg Leu G y 10 aca tcc cac tac tcc cgt cag ctc tcc ctc gtg gac aac gct gag ttc Thr Ser His Tyr Ser Arg G n Leu Ser Leu Val Asp Asn Ala G u Phe 25 30 Page 322 Gene Sequence 24-2-06.txt ggc gag cat tcc ctg cta gaa ggg G y u His Ser Leu Leu u G y tgg act cgt tcc cac ctc att gcc Trp Thr Arg Ser His Leu lie Ala 45 cac Hi s aat Asn gag G u ct g Leu 100 tct Ser gt c Val cac Hi s gt c Val caa G n 180 cgc Ar g ctt Leu act Thr gca Al a ggt Gy at t SIle gaa Gu gat Asp gct Al a gtt Val 150 ct g Leu gcc Al a ccc Pr o ggc Gy t cc Ser aac Asn gaa Gu t ac Tyr t cc Ser tgg Tr p 120 gaa Gu ct c Leu acc Thr gag G u gcc Al a 200 gtt Val gga Gy gcc Al a acc Thr ggt Gy gt c Val 105 t ca Ser aca Thr ggt Gy tta Leu gga Gy 185 cca Pr o cgc Ar g gag Gu at c l Ie cca Pr o t cc Ser gca Al a cac Hi s ttg Leu gca Al a gct Al a 170 ctt Leu ggg Gy t ac Tyr gtt Val gca Al a at g Net 75 acg Thr cgc Ar g gag Gu tgg Tr p gt g Val 155 gca Al a gt g Val cag G n gcc Al a ct g Leu t ac Tyr ct c Leu ct g Leu gtt Val at g Net 140 gca Al a gaa G u ctt Leu gac Asp gct Al a 220 aac Asn tcg Ser ccc Pr o gt g Val 110 aca Thr t cc Ser ttt Phe aca Thr gac Asp 190 gt a Val cgc Ar g ct c Leu cca Pr o gat Asp gct Al a gct Al a cgc Ar g ggc Gy caa G n 175 gag G u gt a Val ggt Gy atg cac Mbt Hi s gaa gcg G u Al a gcg ttg Al a Leu tgg cgt Trp Arg cag gga G n y gaa gt c G u Val 145 gac at c Asp II e 160 aag tgg Lys Tr p ccc t cc Pro Ser gtg tcc Val Ser t cc gat Ser Asp 225 tgg ctg Trp Leu 240 tgg Tr p cgc Ar g cgt Ar g gaa G u cgc Ar g 130 tgg Tr p cca Pr o aca Thr agc Ser ggt Gy 210 gga Gy gca Al a aac Asn aac Asn acg Thr 115 act Thr at c SIe gag G u agc Ser act Thr 195 agc Ser gtc Val cca gag cca ccg cgc Pro u Pro Pro Arg 235 230 tagtttccac acattcttaa atg <210> 250 <211> 241 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 250 lIet Thr Thr Phe His Asp Leu Pro Leu u u Arg Leu Thr Leu Ala 1 5 10 Arg Leu G y Thr Ser His Tyr Ser Arg G n Leu Ser Leu Val Asp Asn 25 Ala G u Phe G y G u His Ser Leu Leu G u Gy Trp Thr Arg Ser His Page 323 Gene Sequence 24-2-06.txt Asn Ala lie Ala Cys Asn Leu lIet Leu Hi s Al a Leu Ar g Gy Val 145 I le Tr p Ser Ser Asp 225 Leu Al a Al a Asn Asn Thr 115 Thr SIle G u Ser Thr 195 Ser Val Val Thr G u Hi s G u Pr o Al a SIle 165 Gy Tyr Al a Ser Al a Gy 70 I le G u Asp Al a Val 150 Leu Al a Pr o Gy Ser 230 Tyr G u Al a Hi s Al a Ser 135 Asp Ar g Gy Al a SIle 215 Thr u Tyr Ser Tr p 120 G u Leu Thr G u Al a 200 Val Gy Thr Gy Val 105 Ser Thr Gy Leu Gy 185 Pr o Ar g G u Pr o Ser 90 Al a Hi s Leu Al a Al a 170 Leu Gy Tyr Val Nbet 75 Thr Ar g G u Tr p Val 155 Al a Val G n Al a Pr o 235 Leu Ty r Leu Leu Val let 140 Al a G u Leu Asp Al a 220 G u Val Asn Asp Leu 125 Ar g Thr SIle Leu G u 205 Gy Pr o Pr o Asp Al a Al a Ar g Gy G n 175 G u Val Gy Ar g u Al a Tr p G n G u Asp 160 Lys Pr o Val Ser Tr p 240 <210> 251 <211> 1575 <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(1552) <223> RXN00999 <400> 251 cctcctgtga cctgg ttgctgacac cgggc aat ctc gca cag Asn Leu Ala G n ctc gcc cgc aac Leu Ala Arg Asn cgc agc act gac jtaaaa tcgccactac ccccaaatgg tcacaccttt taggccgatt t atgc cgtcaagtac gatcaataac atg act aat gga gat IVet Thr Asn G y Asp 1 atc ggc gtt gta ggc cta gca gta atg ggc tca aac I le G y Val Val G y Leu Al a Val Iet G y Ser Asn 15 ttc gcc cgc aac ggc aac act gtc gct gtc tac aac Phe Al a Arg Asn y Asn Thr Val Ala Val Tyr Asn 30 aaa acc gac aag ctc atc gcc gat cac ggc tcc gaa Page 324 Arg Ser Thr Asp Lys Thr Asp ene Sequence 24- 2- 06. txt Lys Leu i e Al aAsp Hi s G y Ser G u ggC G31y g aa O31u g ac As p at c I Ie aag Ly s ggC G31y cca Pr o 1 50 gcc A a gcc A a at g vb t at g vb t g ac As p 230 gt g ValI gca A a ct g Le u ct c Le u tt c Ph e cca Pr o gt c ValI g ac As p at c I Ie 120 g aa O31u aag Ly s gt t ValI c ac Hi s gt c ValI 200 cca Pr o g at As p gct A a c ag O31n at t I Ie 280 ggc G31y cct Pr a cgc Ar g aac As n ggc G31y gca A a ggc G31y t ac Tyr G31y 1 70 gt c ValI ggc G31y g aa O31u t ac Tyr ac c Thr 250 ggc G31y ac c Thr ac c Thr t ct Ser gcc A a 75 cag O31n aac As n cgc Ar g gca A a gag O31u 155 acc Thr aag Ly s gag O31u at c I Ie ct c Le u 235 ggc G31y acc Thr acc Thr ag c Ser gca A a at c I Ie ct g Le u gcc A a ggt G31y ct c Le u 140 t cc Ser cca Pr a at g vb t gca A a gct A a 220 at c I Ie aag Ly s gga G31y ggc G31y c ag O31n 300 ac c Thr at c I Ie gca A a ct c Le u ct c Le u 125 aac As n ct c Le u t gt cys gt c ValI t ac Tyr 205 gag (31u g aa (31u cca Pr a cgt Ar g at c I Ie 285 cgc Ar g gt c ValI at g vb t g at As p t ac Tyr 110 c ac Hi s ggc G31y gga G31y gt c ValI c ac Hi s 1 90 c ac Hi s gt t ValI at c I Ie ct a Le u t gg Tr p 270 ggc G31y gct A a g aa (31u gt t ValI gcc A a 95 ac c Thr t tc Ph e cca Pr a cca Pr a ac c Thr 1 75 aac As n ct t Le u t tc Ph e ac c Thr at c I Ie 255 ac c Thr g aa (31u gca A a gag (31u c ag O31n 80 at g vb t g ac As p gt c ValI t cc Ser ct g Le u 1 60 c ac Hi s ggc O31y ct c Le u aag Ly s gca A a 240 g ac As p gt c ValI gct A a gca A a t tc Ph e gct A a g ac As p ac c Thr ggt O31y at c I Ie 145 ctt Le u at c I Ie at c I Ie cgc Ar g g aa O31u 225 gag O31u gt c ValI aag Ly s gt t ValI c ag O31n 305 gt a ValI ggt O31y g aa O31u at t I Ie gct A a 130 at g vb t gag O31u O31y gag O31u t ac Tyr 210 t gg Tr p gt t ValI at c I Ie gct A a t tc Ph e 290 ggc O31y gca A a aac As n ggc O31y cgt Ar g 115 ggt O31y cct Pr a t cc Ser cca Pr a t ac Tyr 1 gca A a aac As n ct c Le u gt t ValI gct A a 275 gca A a aac As n t cc Ser gcc A a g ac As p 1 00 cgc Ar g at c I Ie ggt O31y at c I Ie g ac As p 1 gcc A a gca A a gca A a t cc Ser g ac As p 260 ctt Le u cgt Ar g ct a Le u ct g Le u ac c Thr at c I Ie gag O31u t cc Ser ggc O31y gct A a 1 ggc O31y g ac As p ggc O31y ggc O31y c ag O31n 245 gct A a g at As p gca A a cct Pr a 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 gca ggt gtc ctc acc gat ctg gaa gca ctt ggc gtg gac aag gca cag Page 325 Ala y Val Leu Thr 310 Gene Sequence 24- 2- 06. txt Leu G u Ala Leu y Val Asp L 320 ys Ala G n 325 ttc Phe t ac Tyr tgg Tr p at c SIle aac Asn 390 ct c Leu cag G n gac Asp cgc Ar g t cc gaa G u cag G n gtt Val 360 cgc Ar g gaa G u gac Asp ggc G y ct g Leu 440 ttc Phe cac gtt Val 330 ttc Phe cct Pr o aag Lys gag G u at c SIle 410 cca Pr o gca Al a ggt Sy gag Su cgc Ar g gac Asp cgc Ar g tt c Phe t cc Ser 395 gat Asp at c SIle gag G u gcg Al a tgg Tr p 475 cgt Ar g gag G u gac Asp ct c Leu 380 ct g Leu t ca Ser cca Pr o cgt Ar g cac Hi s 460 t cc Ser gca Al a at c SIle ct c Leu 365 aac Asn ct g Leu tgg Tr p gt g Val ct g Leu 445 acc Thr ct g Leu aag Lys 350 gct Al a cgc Ar g ct c Leu cgt Ar g ttc Phe 430 cca Pr o t ac Tyr t ac Tyr 335 gct Al a acc Thr at c SIle gat Asp cgc Ar g 415 gct Al a gca Al a aag Lys gca Al a ggc Gy at c SIle gt c Val cct Pr o 400 gt g Val t cc Ser gcc Al a cgc Ar g aag Lys gac Asp cgc Ar g 370 gca Al a ttc Phe gt c Val ct g Leu at c l Ie 450 gac Asp gtt Val 340 aac Asn ggc Gy gat Asp agc Ser gcc Al a 420 t ac Tyr gga G y gat Asp 1123 1171 1219 1267 1315 1363 1411 1459 1507 1552 1575 Ser Phe His Thr 470 ggc gac cgc tcc gag gtt gaa gct Gy Asp Arg Ser G u Val G u Ala 480 taaaggctct ccttttaaca caa <210> 252 <211> 484 <212> PRT <213> Corynebacter i um gl utam cum <400> 252 IVet Thr Asn G y Asp Asn Leu Al a G n 1 5 Val IVet Gy Ser Asn Leu Ala Arg Asn Val Ala Val Tyr Asn Arg Ser Thr Asp 40 Asp Hi s G y Ser G u G y Asn Phe I I e 55 Phe Val Ala Ser Leu G u Lys Pro Arg 70 Ala y Asn Ala Thr Asp Ala Val lie I e G y Val 10 Phe Al a Ar g Lys Thr Asp Pro Ser Ala Arg Ala lle 75 Asn G n Leu Page 326 Val Asn Lys Thr SIle Al a Leu Asn SIle G u Val Al a Al a Thr Al a G u G n bet Gene Sequence 24-2-06.txt G y Asn Al a Leu Asp Thr Gy I e 145 Leu SIle SIle Ar g G u 225 G u Val Lys Val G n 305 Val Ser Ser Tr p G u 385 Tyr SIle Ser Sy Ar g 115 Sy Pr o Ser Pr o Tyr 195 Al a Asn Leu Val Al a 275 Al a Asn Lys Leu G u 355 Sy Tyr Lys Thr Ser 435 SIle G u Ser Sy Al a 165 Sy Asp Sy Sy G n 245 Al a Asp Al a Pr o G n 325 Al a Asn Cys Al a G u 405 Thr Tyr SIle Lys Sy Pr o 150 Al a Al a bet vet Asp 230 Val Al a Leu Leu Al a 310 Phe Ty r Tr p SIle Asn 390 Leu G n Asp SIle G u Gy 135 Al a Asn Gy G n G n 215 Leu Asp Sy Sy Ser 295 Sy Val Al a Asp SIle 375 Al a Sy Leu Ser Sy 105 Ser G u Ser Asp Phe 185 I le Al a Ser G u Lys 265 Al a Al a Leu Asp Sy 345 Asp Al a Leu Leu Leu 425 Ar g Al a Sy Tyr Sy 170 Val Sy G u Tyr Thr 250 Sy Thr Thr Thr Val 330 Phe Pr o Lys G u SIle 410 Pr o Ar g Al a G u 155 Thr Lys Su I e Leu 235 Sy Thr Thr Ser Asp 315 Ar g Asp Ar g Phe Ser 395 Asp SIle Gy Leu 140 Ser Pr o bet Al a Al a 220 I le Lys Sy Sy G n 300 Leu Ar g G u Asp Leu 380 Leu Ser Pr o Leu 125 Asn Leu Cys Val Tyr 205 G u G u Pr o Ar g SIle 285 Ar g G u Al a SIle Leu 365 Asn Leu Tr p Val Leu 445 Tyr 110 Hi s Sy Sy Val Hi s 190 Hi s Val SIle Leu Tr p 270 Sy Al a Al a Leu Lys 350 Al a Ar g Leu Ar g Phe 430 Thr Asp Phe Val Pro Ser Pro Leu 160 Thr His 175 Asn y Leu Leu Phe Lys Thr Al a 240 Ile Asp 255 Thr Val G u Al a Al a Al a Leu G y 320 Tyr Al a 335 Al a y Thr Ile Ile Val Asp Pro 400 Arg Val 415 Al a Ser Al a G u Ar g Pr o Al a Al a Page 327 Gene Sequence 24- 2- 06. txt Leu lie On Gy n Arg Asp Phe Phe y Ala His Thr Tyr Lys Arg 450 455 460 IIe Asp Lys Asp G y Ser Phe His Thr G u Trp Ser G y Asp Arg Ser 465 470 475 480 Su Val u Ala <210> 253 <211> 1537 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1537) <223> FRXA00999 <400> 253 cctcctgtga cctggtaaaa tcgccactac ccccaaatgg ttgctgacac cgggctatgc cgtcaagtac gatcaataac aat Asn ct c Leu cgc Ar g ggc G y gaa G u gac Asp at c SIle aag Lys ggc Gy cca Pr o 150 gca Al a cgc Ar g act Thr ttc Phe cca Pr o gt c Val gac Asp at c SIle 120 gaa G u aag Lys at c I e ttc Phe aaa Lys cct Pr o cgc Ar g aac Asn ggc Gy gca Al a ggc Gy t ac Tyr ggc G y gcc Al a acc Thr tct Ser gcc Al a 75 cag G n aac Asn cgc Ar g gca Al a gag 15u 155 gtt Val cgc Ar g gac Asp gca Al a at c l Ie ct g Leu gcc Al a ggt Gy ct c Leu 140 t cc Ser gt a Val aac Asn aag Lys 45 acc Thr at c SIle gca Al a ct c Leu ct c Leu 125 aac Asn ct c Leu ggc G y ggc G y ct c Leu gt c Val at g et gat Asp t ac Tyr 110 cac Hi s ggc gay gga Gy ct a Leu 15 aac Asn at c SIle gaa G u gtt Val gcc Al a 95 acc Thr ttc Phe cca Pr o cca Pr o gca Al a act Thr gcc Al a gag G u cag G n 80 at g bet gac Asp gt c Val t cc Ser ct g Leu 160 tcacaccttt taggccgatt atg act aat gga gat 11! IVet Thr Asn G y Asp 1 gta atg ggc tca aac 16 Val IVet G y Ser Asn gtc gct gtc tac aac 21 Val Ala Val Tyr Asn gat cac ggc tcc gaa Asp His G y Ser G u ttc gta gca tcc ctg Phe Val Ala Ser Leu gct ggt aac gcc acc Al a G y Asn Al a Thr gac gaa ggc gac atc Asp G u G y Asp Ile 100 acc att cgt cgc gag Thr lie Arg Arg G u 115 ggt gct ggt atc tcc 49! G y Al a G y Ile Ser 130 atc atg cct ggt ggc 54 Ile IVet Pro y y 145 ctt gag tcc atc gct 59! Leu u Ser Ile Ala 165 gcc aac gtt gac ggc acc cca tgt gtc acc cac atc ggc cca gac ggc Page 328 Ala Asn Val Asp gcc A a at g vb t at g vb t g ac As p 230 gt g ValI gca A a ct g Le u ct c Le u gca A a 310 tt c Ph e t ac Tyr t gg Tr p at c I Ie aac As n 390 ct c Le u c ag O31n c ac Hi s gt c ValI 200 cca Pr o g at As p gct A a c ag O31n at t I Ie 280 ggC G31y gt c ValI g aa O31u c ag O31n gt t ValI 360 cgc Ar g g aa O31u g ac As p ggc G31y G31y Th r 1 70 gt c aag Val Lys ggc gag O31y (1u gaa at c O31u I Ie t ac ct c Ty r Leu 235 acc ggc Thr 03 y 250 ggc acc G31y Thr acc acc Thr Thr acc agc Thr Ser acc gat Thr As p 315 gt t cgc Val Ar g 330 ttc gac Phe As p cct cgc Pr o Ar g aag tt c Lys Phe gag t cc O3 u Ser 395 at c gat I I e As p 410 cca at c Pr o I Ie at g vb t gca A a gct A a 220 at c I Ie aag Ly s gga G31y ggC G31y c ag O31n 300 ct g Le u cgt Ar g gag O31u g ac As p ct c Le u 380 ct g Le u t ca Ser cca Pr o ene Sequence 24- 2- 06. txt Pro Cys Val Thr His lie Gly Pro As p (1y 1 gt c ValI t ac Tyr 205 gag O31u g aa O31u cca Pr o cgt Ar g at c I Ie 285 cgc Ar g g aa O31u gca A a at c I Ie ct c Le u 365 aac As n ct g Le u t gg Tr p gt g ValI c ac Hi s 1 90 c ac Hi s gt t ValI at c I Ie ct a Le u t gg Tr p 270 ggc G31y gct A a gca A a ct g Le u aag Ly s 350 gct A a cgc Ar g ct c Le u cgt Ar g tt c Ph e 430 1 75 aac As n ct t Le u tt c Ph e ac c Thr at c I Ie 255 ac c Thr g aa O31u gca A a ct t Le u t ac Tyr 335 gct A a ac c Thr at c I Ie g at As p cgc Ar g 415 gct A a ggc G31y ct c Le u aag Ly s gca A a 240 g ac As p gt c ValI gct A a gca A a ggc G31y 320 gca A a ggc G31y at c I Ie gt c ValI cct Pr o 400 gt g ValI t cc Ser gag O31u t ac Tyr 210 t gg Tr p gt t ValI at c I Ie gct A a tt c Ph e 290 ggc G31y g ac As p aag Ly s g ac As p cgc Ar g 370 gca A a tt c Ph e gt c ValI ct g Le u t ac Tyr 1 gca A a aac As n ct c Le u gt t ValI gct A a 275 gca A a aac As n aag Ly s ct t Le u gag O31u 355 ggc G31y t ac Tyr aag Ly s ac c Thr t cc Ser 435 gcc A a gca A a gca A a t cc Ser g ac As p 260 ct t Le u cgt Ar g ct a Le u gca A a gt t ValI 340 aac As n ggc G31y g at As p ag c Ser gcc A a 420 t ac Tyr g ac As p ggc G31y ggc G31y c ag O31n 245 gct A a g at As p gca A a cct Pr o c ag O31n 325 gct A a aac As n t gc Cys gca A a gag O31u 405 ac c Thr t ac Tyr 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 1315 1363 1411 1459 gac agc ct g cgt gca gag cgt ct g cca gca gcc ct g at c caa gga cag Page 329 Asp Ser Leu Ar g Ala O31u Ar g Leu F 440 445 cgc gac t tc t tc ggt gcg cac acc t Ar g As p Phe Phe G31y Al a Hi s Thr T 455 460 tcc ttc cac acc gag tgg tcc ggc g Ser Phe Hi s Thr O31u Tr p Ser Gy1 y 470 475 <210> 254 <211> 479 <21 2> PRTF <21 3> Cor ynebact er i urn gi ut ani cumn Sequence 24-2- 06. txt ~roAl aAl a Leu Ilie On 0 y On 450 ac aag cgc at c gac aag gat ggc yr Lys Arg lieAsp Lys Asp O1y 465 ac cgc osp Ar g 1 507 1 537 <400> 254 I'bt Thr Asn G31y ValI ValI As p Ph e Al a As p Thr G31y I Ie 145 Le u I Ie I Ie Ar g O31u 225 O31u ValI G31y ValI G31y Al a As n G31y Ar g 115 G31y Pr o Ser Pr o Tyr 1 95 Al a As n Le u ValI As p As n As n O31u Le u Thr li1e O31u Ser O31y Al a 1 65 G31y As p G31y G31y O31n 245 Al a Le u Ar g G31y (31u 70 As p I Ie Lys5 G31y Pr o 150 Al a Al a vb t vb t As p 230 ValI Al a Al a Ser As n Lys5 Al a I Ie (31u G31y 135 Al a As n G31y O31n O31n 215 Le u As p (31y Asn Leu Al a 0 n Ar g Thr 40 Ph e Pr a ValI As p I Ie 120 O31u Lys5 ValI Hi s ValI 200 Pr a As p Al a (31n As n As p I Ie Ar g I Ie O31y 1 Ser O31u Ser As p Ph e 1 li1e Al a Ser O31u Lys5 265 O31y Al a Thr Ser Al a 75 O31n As n Ar g Al a O31u 1 55 Thr Lys5 Ou le Le u 235 (31y ValI Ar g As p Al a li1e Le u Al a O31y Le u 140 Ser Pr a vb t Al a Al a 220 li1e Ly s ValI As n Ly s Thr I Ie Al a Le u Le u 125 As n Le u cys ValI Tyr 205 O31u O31u Pr a Le u As n I Ie O31u ValI Al a Thr Ph e Pr a Pr a Thr 1 As n Le u Ph e Thr I Ie 255 Al a Thr Al a O31u O31n vb t As p ValI Ser Le u 1 Hi s O31y Le u Ly s Al a 240 As p (31y Thr (31y Ar g Tr p Thr Val 270 Page 330 Gene Sequence 24-2-06.txt Lys Ala Ala Leu Asp Leu Gy I e Ala Thr Thr G y Ile G y G u Al a 285 Val G n 305 Val Ser Ser Tr p G u 385 Tyr SIle Ser Leu SIle 465 275 Al a Asn Lys Leu G u 355 Sy Tyr Lys Thr Ser 435 G n Lys Al a Pr o G n 325 Al a Asn Cys Al a G u 405 Thr Tyr G n Sy Leu Al a 310 Phe Ty r Tr p SIle Asn 390 Leu G n Asp Ar g Ser 470 Ser 295 Sy Val Al a Asp SIle 375 Al a Gy Leu Ser Asp 455 Phe Al a Leu Asp Sy 345 Asp Al a Leu Leu Leu 425 Ar g Phe Thr Thr Thr Val 330 Phe Pr o Lys G u SIle 410 Pr o Al a Sy G u Ser Asp 315 Ar g Asp Ar g Phe Ser 395 Asp SIle G u Al a Tr p 475 Ar g G u Al a SIle Leu 365 Asn Leu Tr p Val Leu 445 Thr Sy Al a Al a Leu Lys 350 Al a Ar g Leu Ar g Phe 430 Pr o Tyr Asp Al a Leu Tyr 335 Al a Thr SIe Asp Ar g 415 Al a Al a Lys Ar g <210> 255 <211> 1326 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1303) <223> RXN02596 <400> 255 aaggtatctg ggtgtggata tgccctgcta actggagaaa cttggcccga tcgggtgtct gaaatttcgg caacgccgaa tgtaagttag tgtcgaatgc atg acg gaa tcg aaa IVet Thr G u Ser Lys 1 aat tac gac tta atc gtt gta ggc tcc ggc ctc ttc ggg ctc acc gtg Asn Tyr Asp Leu lie Val Val y Ser y Leu Phe y Leu Thr Val 15 gct gag cgt gca gct agc cag ctg ggt aag aaa gtc ctc atc gtt gaa Ala G u Arg Ala Ala Ser G n Leu y Lys Lys Val Leu Ile Val G u 30 cgc cgc tcg cac ctc ggt ggc aat gct tac tct gaa gca gaa cca gag Arg Arg Ser His Leu G y G y Asn Ala Tyr Ser G u Ala G u Pro G u Page 331 Gene Sequence 24- 2- 06. txt 45 acc ggc att gaa atc cac aaa tac ggc gcg cac ctc ttc cac acc tcc 307 Thr G y lie G u I e His Lys Tyr G y Al a Hi s Leu Phe Hi s Thr Ser 60 aac aca cgc gtg tgg gaa tac gtc aac cag ttc acc agt ttc acc ggc 355 Asn Thr Arg Val Trp G u Tyr Val Asn G n Phe Thr Ser Phe Thr y 75 80 tac cag cac cgc gtc ttc gca atg cac aac ggc acc gcc tac caa ttc 403 Tyr G n His Arg Val Phe Ala Met His Asn G y Thr Ala Tyr G n Phe 95 100 ccc atg gga ctg ggc ctg att aac cag ttc ttc ggc aag tac tac agc 451 Pro IVet y Leu y Leu Ile Asn G n Phe Phe y Lys Tyr Tyr Ser 105 110 115 cca gat gaa gcc cgt gag ctc atc aag gaa cag tct gca gaa atc gat 499 Pro Asp G u Ala Arg G u Leu Ile Lys G u n Ser Ala G u Ile Asp 120 125 130 tcc tcc gac gcc acc aac ctc gaa gaa aag gcc att tcc ctc att ggt 547 Ser Ser Asp Al a Thr Asn Leu G u G u Lys Al a lle Ser Leu I e G y 135 140 145 cgc cca ctt tac gag gca ttc atc cgc gac tac acc gca aag cag tgg 595 Arg Pro Leu Tyr G u Al a Phe lie Arg Asp Tyr Thr Ala Lys G n Trp 150 155 160 165 cag act gat cca aag aac ctc cca gcc ggc aac atc acc cgc ctg cca 643 G n Thr Asp Pro Lys Asn Leu Pro Ala y Asn Ile Thr Arg Leu Pro 170 175 180 gtt cgc tac aac ttc aac aac cgc tat ttc aac gac acc tac gaa ggc 691 Val Arg Tyr Asn Phe Asn Asn Arg Tyr Phe Asn Asp Thr Tyr G u y 185 190 195 ctt ccc aca gac ggc tac gcg gca tgg ttg gaa aag atg gca gag cat 739 Leu Pro Thr Asp G y Tyr Ala Ala Trp Leu G u Lys IVet Ala G u His 200 205 210 gag ctt atc gac gtc cgc ctc gac acc gac tgg ttc gac gtt cgc gat 787 G u Leu Ile Asp Val Arg Leu Asp Thr Asp Trp Phe Asp Val Arg Asp 215 220 225 gac ctc cgc gca agc aac ccc gac gca cct gtg gtc tac acc ggc cca 835 Asp Leu Arg Ala Ser Asn Pro Asp Ala Pro Val Val Tyr Thr y Pro 230 235 240 245 ctc gac ctc tac ttc aac tac gca gag ggc aag ctg gga tgg cgc acc 883 Leu Asp Leu Tyr Phe Asn Tyr Ala G u y Lys Leu y Trp Arg Thr 250 255 260 ctc gac ttt gaa acc gaa gta gta gaa acc ggt gac ttc caa gga acc 931 Leu Asp Phe G u Thr G u Val Val G u Thr y Asp Phe G n y Thr 265 270 275 cca gtg atg aac tac aac gat gcg gac gta cct ttc acc cgc atc cac 979 Pro Val IVet Asn Tyr Asn Asp Ala Asp Val Pro Phe Thr Arg lie His 280 285 290 gag ttc cgt cac ttc cac cca gag cgt gat gac agt tac ccc aag gat 1027 G u Phe Arg His Phe His Pro G u Arg Asp Asp Ser Tyr Pro Lys Asp 295 300 305 aag acc gtc atc atg cgc gag ttc tcc cgt ttc gca gat aac gag gat 1075 Lys Thr Val lie IVet Arg G u Phe Ser Arg Phe Ala Asp Asn G u Asp Page 332 310 315 gag cct tat tac cca atc aac G u Pro Tyr Tyr Pro Ile Asn 330 cag tac cgc ctt ctg gct gct G n Tyr Arg Leu Leu Al a Ala 345 ttc ggc ggt cga ctg ggc acg Phe y y Arg Leu G y Thr 360 atc ggt tct gcg ctg agc atg I e G y Ser Ala Leu Ser IVet 375 380 gaa gaa ggc aca ccg cta gag Su u y Thr Pro Leu u 390 395 ggcatctccc aca <210> 256 <211> 401 <212> PRT <213> Cor ynebact er i um gl ut ar Gene Sequence 24-2-06.txt 320 act cca gac gac cga gac atg ctg Thr Pro Asp Asp Arg Asp IVet Leu 335 340 gaa gag gct gct aat aat aag gt g G u G u Ala Ala Asn Asn Lys Val 350 355 tac cag tac ctc gac atg cac at g Tyr G n Tyr Leu Asp IVet His IVet 365 370 ttt gac aac aag ctg gtg ccg ttc Phe Asp Asn Lys Leu Val Pro Phe 385 cag gaa cgc gga cac taaaaggaag G n u Arg G y His 400 1123 1171 1219 1267 1313 1326 <400> 256 IVet Thr G u Ser 1 Phe G y Leu Thr Val Leu Ile Val G u Ala G u Pr o Leu Phe His Thr Thr Ser Phe Thr Thr Ala Tyr G n 100 G y Lys Tyr Tyr 115 Ser Ala G u li e 130 Ile Ser Leu Ile 145 Thr Ala Lys G n Ile Thr Arg Leu 180 Lys Asn Tyr 5 Val Ala G u G u Arg Ar g G u Thr G y Ser Asn Thr 70 G y Tyr G n Phe Pro IVet Ser Pro Asp Asp Ser Ser 135 G y Arg Pr o 150 Trp G n Thr 165 Pro Val Ar g Asp Leu Ile 10 Ar g Al a Al a 25 Ser Hi s Leu 40 lie G u lle Arg Val Trp His Arg Val 90 G y Leu G y 105 G u Al a Ar g 120 Asp Al a Thr Leu Tyr G u Asp Pro Lys 170 Tyr Asn Phe 185 Val Ser Sy Hi s G u Phe Leu G u Asn Al a 155 Asn Asn Sy Leu Asn Tyr Val vet Asn SIle 125 Su l e Pr o Ar g Ser Sy Al a Sy Asn Hi s G n 110 Lys G u Ar g Al a Tyr 190 Sy Lys Tyr Al a G n Asn Phe G u Lys Asp Gy 175 Phe Asp Thr Tyr u Gy Leu Pro Thr Asp Gy Tyr Ala Ala Trp Leu u Page 333 Gene Sequence 24-2-06.txt Lys Phe 225 Val Leu Asp Phe Ser 305 Al a Ar g Asn Asp Leu 385 Hi s Al a Val Thr Tr p G n 275 Ar g Pr o Asn Met Lys 355 Hi s Pr o Hi s Asp Pr o 245 Thr Thr Hi s Asp Asp 325 Lys Leu Al a Phe Leu 215 Leu Asp Asp Val Phe 295 Thr Pr o Tyr Gy Gy 375 G u 200 i e Asp Val Ar g Al a Ser Leu Tyr Phe 250 Phe G u Thr 265 IVet Asn Tyr 280 Arg His Phe Val Il e bet Tyr Tyr Pro 330 Arg Leu Leu 345 G y Arg Leu 360 Ser Ala Leu G v Thr Pro 205 Asp Thr Asp Trp Arg Leu 220 Asn Pro 235 Asn Tyr G u Val Asn Asp His Pr o 300 Arg G u 315 i e Asn Al a Al a G y Thr Ser lbet 380 Leu G u 395 Asp Al a Val Al a 285 G u Phe Thr G u Tyr 365 Phe G n Al a G u G u 270 Asp Ar g Ser Pr o G u 350 G n Asp G u Pr o Gy 255 Thr Val Asp Ar g Asp 335 Al a Tyr Asn Ar g <210> 257 <211> 512 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> <223> FRXA02596 <400> 257 cct gtg gtc tac acc ggc cca ctc gac Pro Val Val Tyr Thr G y Pro Leu Asp 1 5 ggc aag ctg gga tgg cgc acc ctc gac Gy Lys Leu Gy Trp Arg Thr Leu Asp 25 acc ggt gac ttc caa gga acc cca gtg Thr Gy Asp Phe n G y Thr Pro Val 40 gta cct ttc acc cgc atc cac gag ttc Val Pro Phe Thr Arg lie His G u Phe 55 ctc tac ttc aac tac gca gag Leu Tyr Phe Asn Tyr Al a u 10 ttt gaa acc gaa gta gta gaa Phe G u Thr G u Val Val G u atg aac tac aac gat gcg gac IVet Asn Tyr Asn Asp Ala Asp cgt cac ttc cac cca gag cgt Arg His Phe His Pro G u Arg Page 334 gat Asp cgt Ar g gac Asp gct Al a t ac Tyr aac Asn 145 cgc Ar g agt Ser gca Al a cga Ar g aat Asn 115 gac Asp ct g Leu cac Hi s Gene Sequence 24-2-06.txt tac ccc aag gat aag acc gtc atc atg cgc gag Tyr Pro Lys Asp Lys Thr Val lie IVet Arg G u 70 gat aac gag gat gag cct t at tac cca at c aac Asp Asn G u Asp G u Pro Tyr Tyr Pro lie Asn 90 gac atg ctg aag cag tac cgc ctt ctg gct gct Asp IVet Leu Lys G n Tyr Arg Leu Leu Ala Ala 100 105 110 aat aag gtg ctg ttc ggc ggt cga ctg ggc acg Asn Lys Val Leu Phe Gy Gy Arg Leu Gy Thr 120 125 atg cac atg gct atc ggt tct gcg ctg agc atg Iet His Iet Ala lle G y Ser Ala Leu Ser iet 135 140 gtg ccg ttc ttt gaa gaa ggc aca ccg cta gag Val Pro Phe Phe G u G u Gy Thr Pro Leu G u 150 155 taaaaggaag ggcatctccc aca ttc Phe act Thr gaa G u t ac Tyr ttt Phe cag G n <210> 258 <211> 163 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 258 Pro Val Val Tyr Thr G y Pro Leu Asp 1 5 Gy Lys Leu Gy Trp Arg Thr Leu Asp Thr y Asp Phe n G y Thr Pro Val 40 Val Pro Phe Thr Arg Il e His G u Phe Asp Asp Ser Tyr Pro Lys Asp Lys Thr 70 Arg Phe Ala Asp Asn G u Asp G u Pro Asp Asp Arg Asp IVet Leu Lys G n Tyr 100 105 Ala Ala Asn Asn Lys Val Leu Phe y 115 120 Tyr Leu Asp IVet His IVet Ala IIe G y 130 135 Asn Lys Leu Val Pro Phe Phe G u G u 145 150 Arg G y His Tyr Phe Asn Tyr G u Asn Hi s SIle Tyr Leu Ar g Al a Thr 155 G u Asn Hi s Ar g SIle Al a Sy 125 Ser Leu Al a Val Al a G u Phe Thr G u Tyr Phe G n Page 335 Gene Sequence 24-2-06.txt <210> 259 <211> 598 <212> DNA <213> Corynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(598) <223> FRXA02642 <400> 259 aaggtatctg ggtgtggata tgccctgcta actggagaaa gaaatttcgg caacgccgaa tgtaagttag tgtcgaatgc ct t ggcccga t cgggt gt ct atg acg gaa tcg aaa 11! lMt Thr G u Ser Lys aat Asn gct Al a cgc Ar g acc Thr aac Asn t ac Tyr ccc Pr o cca Pr o t cc Ser cgc Ar g 150 cag G n gac Asp cgt Ar g tcg Ser at t SIle cgc Ar g cac Hi s gga G y gaa G u 120 gac Asp at c I e gct Al a ct c Leu at c SIle tgg Tr p gt c Val ggc G y cgt Ar g acc Thr gtt Val agc Ser ggt G y cac Hi s gaa G u 75 tt c Phe ct g Leu gag G u aac Asn gt a Val cag G n ggc G y aaa Lys t ac Tyr gca Al a at t SIle ct c Leu ct c Leu 140 ggc Gy ct g Leu aat Asn 45 t ac Tyr gt c Val at g et aac Asn at c SIle 125 gaa G u t cc Ser ggt Gy gct Al a ggc G y aac Asn cac Hi s cag G n 110 aag Lys gaa G u ggc Gy 15 aag Lys t ac Tyr gcg Al a cag G n aac Asn 95 ttc Phe gaa G u aag Lys ct c Leu aaa Lys tct Ser cac Hi s ttc Phe G y ttc Phe cag G n gcc Al a 1 ttc Phe gt c Val gaa G u ct c Leu acc Thr acc Thr ggc G y tct Ser at t l Ie 145 ggg G y ct c Leu gca Al a ttc Phe agt Ser gcc Al a aag Lys gca Al a 130 t cc Ser ct c Leu at c SIle gaa G u cac Hi s ttc Phe t ac Tyr t ac Tyr 115 gaa G u ct c Leu acc Thr gtt Val cca Pr o acc Thr acc Thr caa G n 100 t ac Tyr at c SIe att SIe cca ctt tac gag gca Pro Leu Tyr u Al a 155 ttc atc cgc gac tac Phe Ile Arg Asp Tyr 160 acc gca aag cag Thr Ala Lys G n <210> 260 <211> 166 <212> PRT <213> Corynebacter i um gl utami cum Page 336 Gene Sequence 24-2-06.txt <400> 260 Net 1 Phe Val G u Leu Thr Thr Gy Ser SIle 145 Thr G u Leu I e G u Hi s Phe Tyr Tyr 115 G u Leu Lys Lys 5 Val G u G u Ser Gy Phe Ser Asp Gy Tr p 165 Asn Al a Ar g Thr Asn 70 Ty r Pr o Pr o Ser Ar g 150 G n Tyr G u Ar g Gy Thr G n et Asp Ser 135 Pr o Leu II e 10 Al a Al a Hi s Leu G u Ile Val Trp Arg Val 90 Leu G y 105 Al a Ar g Al a Thr Tyr G u Val Val y Ser Ser Gy Hi s G u Phe Leu G u Asn Al a 155 Leu Asn Tyr Val bet Asn SIle 125 Su l e gcg Al a aag Lys gac Asp gca Al a gac Asp ggc Gy Al a Gy Asn Hi s G n 110 Lys G u Ar g gtt Val ttc Phe at c SIle tta Leu cgt Ar g aag Gy Lys Tyr Al a G n Asn Phe G u Lys Asp gcg Al a tta Leu cgc Ar g acg Thr gtg Val cgg <210> 261 <211> 668 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> RXA02572 <400> 261 gcg gtc gct gag att tgc gag ccg acc Ala Val Ala u Ile Cys u Pro Thr 1 5 gtg gat gcc atc ggt cac gac gat cgt Val Asp Ala lie G y His Asp Asp Arg 25 gcg ggc ctg gga ttc ggt ggc ggt tgt Al a y Leu G y Phe G y G y G y Cys 40 ttc atg gca cgc gcg ggc gaa ttg ggt Phe IVet Ala Arg Ala Gy u Leu y 55 ttg cgt gag gtc gat tcc atc aat atg Leu Arg G u Val Asp Ser lie Asn IVet 70 cag ctg gcc aaa gag atg tgt ggc ggt ggc gcc gat G y Ala Asp 10 atc ggc cga lie y Arg ttg cct aaa Leu Pro Lys gct gac cag Al a Asp G n cgt cgt cgc Arg Arg Arg tcg ctg ctg Page 337 G n Leu Ala Lys G u IVet Cys Gene Sequence 24-2-06. t xt Gy y Ser Leu Leu y Lys Arg Val aca gtg ctc ggc gcc gca ttc aaa ccc Thr Val Leu Gy Ala Ala Phe Lys Pro 100 105 tct ccg gcg ctg tcg gtc gcg ggt tcg Ser Pro Ala Leu Ser Val Ala y Ser 115 120 gtc tcg gtc tac gac ccg gaa gct atg Val Ser Val Tyr Asp Pro G u Ala Mbet 130 135 ccg acg ctc agc tat gcg tcc agc act Pro Thr Leu Ser Tyr Al a Ser Ser Thr 145 150 cac ctc gtc gtt ctt gcc act gaa tgg His Leu Val Val Leu Al a Thr G u Trp 165 ccc gaa gtg gcg gga ggg gtc gtc gag Pro u Val Ala Gy y Val Val u 180 185 cga aac gtc ctc gat gtt gcc aaa tgg Arg Asn Val Leu Asp Val Ala Lys Trp 195 200 gaa gcg ctc ggc cgc aac ctt tagtgcg! G u Ala Leu Gy Arg Asn Leu 210 215 <210> 262 <211> 215 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 262 Ala Val Ala Gu Ile Cys Gu Pro Thr 1 5 Val Asp Ala lie G y His Asp Asp Arg Al a y Leu G y Phe G y G y G y Cys 40 Phe IVet Ala Arg Ala y u Leu y 55 Leu Arg G u Val Asp Ser Ile Asn IVet 70 Sn Leu Ala Lys u et Cys y y Thr Val Leu y Ala Al a Phe Lys Pro 100 105 Ser Pro Ala Leu Ser Val Ala y Ser 115 120 Val Ser Val Tyr Asp Pro G u Ala I\et 130 135 aac Asn ct g Leu gac Asp aaa Lys caa G n 170 aag Lys aag Lys tcg Ser tcg Ser aac Asn gag G u 155 gaa G u cgc Ar g gcc Al a gac Asp ct c Leu gct Al a 140 gcg Al a ttc Phe gct Al a gcc Al a gat gl Asp V, 1 cag g! G n G 125 cga c! Arg Ai ctt al Leu I I cgc gi Arg A att al lie II ggt t G y Ti 205 cgc Ar g gcg Al a gt c Val gac Asp ctt Leu 175 gat Asp gaa G u gat Asp gcg Al a ttc Phe gcc Al a 160 gac Asp ggc G y at g bet gtg gatcaggcgg ggc G y Al a Asp Al a V, 10 lie Gy Arg Lys PI Leu Pro Lys Asp II Ala Asp n Ala Li Arg Arg Arg Asp Ai 75 Ser Leu Leu G y L. 90 Asn Ser Asp Asp V, 1 Leu Ser Leu G n G 125 Asp Asn Ala Arg Al 140 Page 338 Al a Leu Ar g Thr Val Ar g Ar g Al a Val Leu Sy Al a Phe Val Val Asp Al a Phe Gene Sequence 24 Pro Thr Leu Ser Tyr Al a Ser Ser Thr Lys G u 145 150 155 His Leu Val Val Leu Al a Thr Gu Trp G n u 165 170 Pro G u Val Ala Gy G y Val Val G u Lys Arg 180 185 Arg Asn Val Leu Asp Val Ala Lys Trp Lys Ala 195 200 G u Ala Leu y Arg Asn Leu 210 215 <210> 263 <211> 1224 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1201) <223> RXA02485 <400> 263 cggtggtcag tgcttggtgc accttgccga cgggctgatt -2-06. txt Ala Leu Ile Asp Ala 160 Phe Arg Asp Leu Asp 175 Al a lIe Ile Asp G y 190 Ala y Trp Gu bet 205 gatcgtaatg gtgttttctg tacgcgttgc catgaggata agactaccgt t agt ggggt g gcc Al a act Thr gcc Al a ctt Leu ct c Leu acc Thr gca Al a ct c Leu acc Thr gaa Gu gcc Al a cgt Ar g gac Asp gt g Val gac Asp gct Al a gga Gy 120 gt c Val gcc Al a ct g Leu cag G n t cc Ser gac Asp t cc Ser tgg Tr p gaa G u aac Asn gcg Al a acg Thr acc Thr ct c Leu ggc Gy 75 at c SIle gac Asp tgc Cys gt g Val at c l Ie acc Thr acg Thr ccc Pr o gat Asp aac Asn gat Asp ct c Leu ggc Gy ggc Gy cgc Ar g gcg Al a ct c Leu gat Asp acc Thr gt c Val 110 gga Gy t ac Tyr gaa G u ggc Gy gt c Val gt c Val at t SIle 80 ggt Gy ctt Leu ccc Pr o gat tca tcg cta Asp Ser Ser Leu gat tcg gaa gtc Asp Ser G u Val aaa ccc cgc agc Lys Pro Arg Ser gcc ata aaa ttg Ala lle Lys Leu ggc ggg tcc aat Gy y Ser Asn gtc atc atc gaa Val Ile lle u ctc acc gcc gat Leu Thr Ala Asp 100 gtg gat gcc ggc Val Asp Ala y 115 tcc gcc ggc gcc Ser Al a y Al a 130 ggc acg gaa gtt tcc gat gta Sy Thr G u Val Ser Asp Val Page 339 ene Sequence 24- 2- 06. t xt 145 cac caa gt c t cc His O31n ValI Ser ct C Le u 1 50 acc cgc gt c cag Thr Ar g Val O31n ct C gac cgc acc Leu Asp Arg Thr ac c Th r 1 60 t gg Tr p gt c gac gcc t cc Val Asp Al a Ser g aa O31u 1 70 ct c gac ct c t ct Leu Asp Leu Ser cga t ac t cc aat Arg Tyr Ser Asn 165 ct c aaa Leu Lys 1 t tc acc aac Phe Thr Asn gac gga tt g Asp 03 y Leu 200 gca gt c gt c tt g Al a Val Val Leu gcg Al a 1 90 at c gaa ct c cag Il e 03 u Leu 03 n ct c ct c acc Leu Leu Thr 1 cgt cga t ta Ar g Ar g Leu t cc gcg ccg ct a Ser Al a Pr o Leu t tt ggt gaa tt g Phe 03 y 03 u Leu gga G31y 210 gcg at c Al a IlIe 215 t cc gag gcc gaa Ser 03 u Al a 03 u ccc Pr o 220 cac cca cgt cgc Hi s Pr o Ar g Ar g gt c cgc at g gt c Val Ar g I'b t Val cgc Ar g 230 gac gcc gt c ct a Asp Al a Val Leu gaa O31u 235 ct c cgc cgc gcc Leu Ar g Ar g Al a aaa Lys5 240 ggc at g gt c gt g G31y I'b t Val Val cac acc gac cac Hi s Thr Asp Hi s g ac Asp 250 acc tgg tcc gcc Thr Tr p Ser Al a gga G31y 255 tcc ttc ttc acc Ser Phe Phe Thr aac cca Asn Pr o 260 at c gt c gac Ilie Val Asp ccc acc at g Pr o Thr I'b t 280 gcc ct t gcc gac Al a Leu Al a Asp gca Al a 270 gt c t tt gaa aaa Val Phe 03 u Lys gt c ggc gaa Val O31y (1u 275 aaa ct c t cc Lys Leu Ser ccc cgc ttc cca Pr o Ar g Phe Pr o gcc Al a 285 ggc gat ggc aaa 03 y Asp 03 y Lys g aa 031u 290 gca gcc Al a Al a 295 t gg ct c at c gaa Tr p Leu Ilie 031u cgc Ar g 300 gcc ggc ttc aaa Al a 03 y Phe Lys gga cac ccc ggc G31y Hi s Pr o G31y gca Al a 310 ggc gca aaa gcc 03 y Al a Lys Al a ct g agc acc aaa Leu Ser Thr Lys c ac Hi s 320 acc ct c gca ct c Thr Leu Al a Leu aac cgt ggc gac Asn Ar g G31y Asp gcc Al a 330 cgc gcc tcc gac Ar g Al a Ser Asp gtc gca tta gcc Val Al a Leu Al a aaa gaa Lys 03 u 340 1 027 1 075 1123 1171 1221 1224 at c cgc gac Ilie Ar g Asp ccc gt c t gg Pr o Val Tr p 360 gt c ct c gaa acc Val Leu 0 u Thr ttc Ph e 350 ggc gt c acc ct c 03 y Val Thr Leu gt c cca gaa Val Pr o O31u 355 at t gga at c agc Il e 03 y Il e Ser at c gat gac Il e Asp Asp 365 t gaatt t tcc gacgt ccct g gca <210> 264 <211> 367 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 264 Leu Asp Ser Ser Leu Ala(3n Ou lleAlaAlalleAspOy Val Ou Page 340 Gene Sequence 24-2-06.txt Leu Gy Ser Gy Al a Leu Ser Gy G u 145 Hi s Tyr Leu Leu Pr o 225 Gy Phe G u Lys Lys 305 Thr Al a Thr Ser Pr o I le Gy l Ie Thr Asp 115 Al a Ser Val Asn Leu 195 Ar g Ar g Val Thr Val 275 Lys Hi s Al a Al a Val 355 Val Ser Leu Asn G u Asp Gy Al a Val Tr p 165 Lys Thr Leu Val G u 245 Pr o G u Ser Gy Thr 325 G u G u Thr Al a Leu Leu 70 Thr Al a Leu Thr Leu 150 Val Phe Asp Al a Ar g 230 Hi s SIle Pr o Al a Al a 310 Asn SIle Pr o Phe Val Asp Val Asp Gy Gy Pr o 135 Thr Asp Thr Gy l Ie 215 Asp Thr Val Thr Al a 295 Gy Ar g Ar g Val Al a Ar g 40 Asp Val Asp Al a Gy 120 Val Ar g Al a Asn Leu 200 Ser Al a Asp Asp vet 280 Tr p Al a Gy Asp Tr p 360 Asp Leu Thr Cys G n Thr Al a Ser Leu Al a Asp G y Val Ser Ile 90 Val Trp Asp 105 Ile G u Cys G n Asn Val Val G n Leu 155 Ser G u Leu 170 Ar g Al a Val 185 Ser Ala Pro G u Al a u Val Leu G u 235 Hi s Asp Thr 250 Pro Al a Leu 265 Pro Arg Phe Leu Ile G u Lys Al a Ser 315 Asp Al a Ar g 330 G y Val Leu 345 lie G y lle Thr Leu Arg Thr G u Al a Pro Leu Leu Asp Leu Asp Asn Leu Thr Asp Val Val 110 Leu Ser G y 125 G y Al a Tyr 140 Leu Asp Arg Asp Leu Ser Val Leu Al a 190 Leu Arg Phe 205 Pro His Pro 220 Leu Arg Arg Trp Ser Ala Al a Asp Al a 270 Pr o Al a G y 285 Ar g Al a G y 300 Leu Ser Thr Al a Ser Asp G u Thr Phe 350 SIe Leu SIe Val Asp Hi s SIe Sy Thr Tyr 175 l e Sy Ar g Al a Sy 255 Val Asp Phe Lys Leu 335 Sy Ser Ile Asp Asp 365 Page 341 ene Sequence 24- 2- 06. txt <210O> 265 <211> 1124 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1101) <223> RXA01216 <400> 265 ac c Th r ac c Th r G31y c aa (31n ggt G31y ct g Le u ggc G31y g at As p gcc A a ggc G31y 145 t ct Ser g aa O31u ac c Th r tt c Ph e C ac Hi s gt c ValI at c I Ie g at As p tt t Ph e t cc Ser gct A a cgt Ar g 115 gcc A a ac c Th r gga G31y gt c ValI at g vb t 1 95 t cc Ser ct g Le u gt g ValI cgc Ar g t ca Ser tt c Ph e aac As n ggc G31y ct c Le u ct a Le u gt t ValI g ac As p 1 65 gga G31y at c I Ie at c I Ie t ct gca ct g ct g Ser A a Leu Leu t t g Le u aac As n gca A a gac As p 70 gct A a gag (31u gcc A a aac As n g at As p 150 gt g ValI gac As p ggc G31y ggt G31y ac c Th r g aa (31u g aa (31u gcc A a c ag O31n G31y G31y cgt Ar g 135 cca Pr o at c I Ie cgc Ar g g ac As p g aa (31u 215 cgt Ar g ggc G31y c aa (31n at c I Ie gag (31u cca Pr o 1 aac As n ac c Th r ac c Th r c ac Hi s g aa (31u 1 gca A a gcc A a g at As p 10 ct g Le u g aa (31u gcc A a tt g Le u ct g Le u 90 gt g ValI g at As p gt c ValI ac c Th r cct Pr o 1 70 gt t ValI t cc Ser ac c Th r gca cac gt g gaa Ala Hi s Va1(3 Ou g at As p gt c ValI at c I Ie cgt Ar g 75 t ac Ty r cgc Ar g cgt Ar g at c I Ie t gg Tr p 1 55 ggc G31y ggt G31y gt a ValI gt t ValI CCC Pr a gcc A a gag G u gca A a ac c Th r c ac Hi s c ag O3n 125 gct A a g at As p c ag O3n g ac As p cgc Ar g 205 CCC Pr a gt t cca Val Pr o ggc t ac O3 y Ty r gt t gag Val O31u aac t CC Asn Ser gct gaa A a 03 u gt t tt g Val Leu gcc gcc A a A a gca gaa A a 03 u cgt ggt Ar g O31y gag gtt O3 u Val 1 aag ggc Lys 03 y 1 acc tt g Thr Leu cac ggt Hi s 03 y acc t ac Thr Tvr Page 342 Gene Sequence 24-2-06.txt at c SIle 225 gt a Val ct c Leu gcc Al a acc Thr cca Pr o 305 aaa Lys cga Ar g gca Al a cca gga acc aca ctg gga cca gaa Pro y Thr Thr Leu y Pro u acc Thr t at Tyr tct Ser 275 ggc Gy acc Thr gat Asp at c SIle gcc Al a aag Lys 245 ggc Gy ttc Phe cac Hi s ggt Gy ccg Pr o 325 ggc Gy gaa G u 230 gcc Al a gac Asp gt g Val gtt Val gac Asp 310 cca Pr o tgg Tr p gcc Al a aca Thr gcc Al a aac Asn cgc Ar g 295 gga Gy gga Gy gt g Val gcc Al a at c SIle acc Thr t ac Tyr 280 act Thr gcg Al a gcc Al a caa G n caa G n 360 ggc Gy at c SIle 265 gac Asp ggt Gy t at Tyr ctt Leu aag Lys 345 aac Asn cgt Ar g 250 ggc Gy ggt Gy tct Ser t cc Ser gcc Al a 330 aag Lys gt c Val ggc Gy 235 ggc Gy gag G u gaa G u gac Asp gga Gy 315 gt g Val cgc Ar g cac Hi s t cc Ser gaa G u aac Asn acc Thr 300 gcc Al a t cc Ser cct Pr o aac Asn aag Lys t cc Ser aag Lys 285 at g bet ggt Gy ggc Gy gga Gy cag G n 365 gtt Val aac Asn 270 cac Hi s ttt Phe aca Thr gga Gy acc Thr 350 gaa G u cca Pr o 255 at c SIle cac Hi s at c SIle gt a Val cgc Ar g 335 gct Al a ggc Gy aag ctc ggt ggc ttc Lys Leu G y G y Phe 240 cac Hi s gga Gy acc Thr gct Al a at t SIe 320 caa G n gca Al a 768 816 864 912 960 1008 1056 1101 1124 355 taagcaggat cctcatgact gct <210> 266 <211> 367 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 266 Thr Asp His Thr Leu Ser Ala Leu Leu 1 5 Thr Ala Val Thr Val Leu Thr et Arg Gy Arg lie Val Arg Asn Gu Gu Gy 40 G n Lys Asp Ala Ser Al a G u Val G n 55 Sy Val Phe Ala Phe Asp Ala Ala lie 70 Leu Lys Ser Asp Asn Al a G n y G u Sy lie Ala Arg y u y His Pro 100 105 Asp Al a Arg G u Leu Al a G y Val Asn 115 120 Asp Al a Hi s 10 Leu Asp Asp G u Val Thr Al a lie Asp Leu Arg Ser 75 Leu Tyr Leu 90 Val Arg Ala Asp Arg Val Page 343 Val Pr o Al a G u Al a Thr Hi s G n 125 Val Sy Val Asn Al a Val Al a Al a Pr o Tyr G u Ser G u Leu Al a G u Gene Sequence 24-2-06.txt Al a Gy 145 Ser G u Thr Phe SIle 225 Val Leu Al a Thr Pr o 305 Lys Ar g Al a Al a Thr Gy Val et 195 Ser Pr o Thr Tyr Ser 275 Gy Thr Asp SIle Al a 355 Leu Val Asp 165 Gy I e I e Thr Lys 245 Gy Phe Hi s Gy Pr o 325 Gy Arg Arg Thr 135 Pro Al a Thr Ile Il e Hi s Arg Val G u 185 Asp G y Al a 200 G u Asn Ala 215 Leu G y Pro Thr I e G y Ala Thr Ile 265 Asn Tyr Asp 280 Arg Thr G y 295 G y Al a Tyr G y Al a Leu Val G n Lys 345 Val Ile Ala Ala Met Arg y Thr Pr o 170 Val Ser Thr G u Ar g 250 Gy Sy Ser Ser Al a 330 Lys Tr p 155 Sy Sy Val Val Sy 235 Sy G u G u Asp Sy 315 Val Ar g Asp G n Asp Ar g 205 Pr o Leu Lys Ser Lys 285 bet Sy Sy Sy G u Lys 175 Thr Hi s Thr Sy Pr o 255 I e Hi s SIe Val Ar g 335 Al a Ala G u Ala Ala n Asn Val His Asn Gn u y 360 365 <210> 267 <211> 981 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(958) <223> RXA01259 <400> 267 aagagaatta tttctaaaat tcggtatcgt ctaagaaatg agtttgccaa tagctcagca tcaaaatgct gtaaaaactg tcgtggtacc agctgcagga atg gga aca cgg ttc 115 IVet G y Thr Arg Phe 1 ctt cct gca acg aag aca att cca aag gag ctt ctt cct gta gtt gat 163 Leu Pro Ala Thr Lys Thr lie Pro Lys u Leu Leu Pro Val Val Asp 15 Page 344 Gene Sequence 24- 2- 06. txt acc ccg ggt att gaa ctt gtt gcc aaa gag gct gct gat ctt ggt gca 211 Thr Pro Gy I le u Leu Val Ala Lys G u Ala Ala Asp Leu Gy Ala 30 act cgg tta gca att atc act gct ccg aac aaa gac gga att ctt aaa 259 Thr Arg Leu Ala lie lie Thr Ala Pro Asn Lys Asp Gy lie Leu Lys 45 cac ttc gag gag ttc cct gag ctt gag gca act ctt gag gct cgc ggt 307 His Phe G u G u Phe Pro G u Leu G u Ala Thr Leu G u Ala Arg G y 60 aag act gat caa ctg aat aaa gtt cga gca gct cga gaa ttg att gca 355 Lys Thr Asp G n Leu Asn Lys Val Arg Ala Ala Arg G u Leu lie Ala 75 80 aca gtt cca gtg gtt caa gaa aag cca ttg ggg ctt ggt cac gct gtt 403 Thr Val Pro Val Val G n G u Lys Pro Leu G y Leu G y His Ala Val 95 100 ggc ctt gct gag tct gtg ctc gat gat gat gaa gat gtt gtg gct gtc 451 Sy Leu Ala G u Ser Val Leu Asp Asp Asp G u Asp Val Val Ala Val 105 110 115 atg ctg cca gac gat ttg gtg ctg cca ttt ggt gtg acc gag aga atg 499 IVet Leu Pro Asp Asp Leu Val Leu Pro Phe G y Val Thr G u Arg IVet 120 125 130 gca gaa gtt cgc gct aag ttt ggc gga tct gtt ctt gca gca att gag 547 Ala G u Val Arg Ala Lys Phe Gy y Ser Val Leu Ala Ala lie G u 135 140 145 gtg gct gaa gat gaa gtc tca aat tac gga gta ttt aag ctc ggt gaa 595 Val Ala G u Asp G u Val Ser Asn Tyr Gy Val Phe Lys Leu G y u 150 155 160 165 ctc gat gca gag tcc gaa agt gaa ggc att agg cgt gtt gta gga atg 643 Leu Asp Ala u Ser u Ser G u Gy Ile Arg Arg Val Val Gy IVet 170 175 180 gtt gaa aag cct gcg cct gaa gat gca cca tca agg ttt gcc gca acg 691 Val G u Lys Pro Ala Pro G u Asp Ala Pro Ser Arg Phe Ala Ala Thr 185 190 195 ggc cgt tat cta ctt gat cga gct att ttt gat gca ctg cgt cga att 739 Sy Arg Tyr Leu Leu Asp Arg Ala lie Phe Asp Ala Leu Arg Arg lie 200 205 210 gag cct ggt gct ggt gga gaa ctg caa tta aca gat gcc atc gca tta 787 G u Pro G y Ala G y G y G u Leu n Leu Thr Asp Al a lie Al a Leu 215 220 225 ttg atc gaa gaa ggc cat ccg gta cac att gtg gtt cat gaa gga aag 835 Leu I I e u G u G y His Pro Val His Ile Val Val His G u G y Lys 230 235 240 245 cgc cat gac ctt ggt aat cca gct ggg tac att cct gct gtt gtg tac 883 Arg His Asp Leu G y Asn Pro Ala G y Tyr lie Pro Ala Val Val Tyr 250 255 260 ttc gga ctt cgt cat gca gag tac ggt tcc aag att cac cgt gcg gtg 931 Phe G y Leu Arg His Al a G u Tyr G y Ser Lys IIe His Arg Ala Val 265 270 275 aag gaa ata ctc gct gag ttt gaa tct taaaaaggaa accgccttcc 978 Lys G u Ile Leu Ala G u Phe G u Ser 280 285 Page 345 Gene Sequence 24- 2- 06. txt aca <210> 268 <211> 286 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 268 Met G y Thr Ar g 1 Leu Pro Val Val Ala Asp Leu G y Asp G y Ile Leu Leu G u Ala Arg Arg u Leu Ile Leu G y Hi s Al a 100 Asp Val Val Al a 115 Val Thr G u Arg 130 Leu Ala Ala lle 145 Phe Lys Leu G y Arg Val Val y 180 Arg Phe Ala Ala 195 Ala Leu Arg Arg 210 Asp Ala lie Al a 225 Val His G u G y Pro Ala Val Val 260 Ile His Arg Ala 275 Phe 5 Asp Al a Lys Sy Al a Val Val Met G u G u 165 Met Thr SIle Leu Lys 245 Tyr Pr o Pr o Ar g Phe Thr Val Leu Leu G u 135 Al a Asp G u Ar g Pr o 215 I le Hi s Sy Al a Sy Leu 40 G u Asp Pr o Al a Pr o 120 Val G u Al a Lys Tyr 200 Sy G u Asp Leu Thr SIle Al a G u G n Val G u 105 Asp Ar g Asp G u Pr o 185 Leu Al a G u Leu Ar g 265 Thr Leu l Ie Pr o Asn G n Val Leu Lys Val 155 G u Pr o Asp Sy Hi s 235 Asn Al a Pr o Al a Al a Leu Val Lys Asp Leu 125 Sy Asn G u Asp Al a 205 Leu Val Al a Tyr G u G u Asn Al a Al a Leu Asp Phe Ser Gy I e 175 Pr o Phe Leu SIe Tyr 255 Ser Val Lys u lie Leu Ala u Phe u Ser 280 285 <210> 269 <211> 526 <212> DNA <213> Corynebacteri um gl utam cum Page 346 Gene Sequence 24-2-06.txt <220> <221> CDS <222> (101)..(526) <223> RXA02028 <400> 269 tgcgcagttc ctgcttagtt tggctcataa atctaaggat aaccgttatt ttcggagggg tacgacgatt ggggttgcgg gggcaggtac tcttggttcc atg agt ttg cct atc 115 IVet Ser Leu Pro Ile 1 gat gag cac gtg aac gcg gtt aaa acc gtc gta gtg cct gct gca gga 163 Asp G u His Val Asn Al a Val Lys Thr Val Val Val Pro Ala Ala G y 15 ctg gga acc cga ttc ctt ccg gcc acc aaa acc gta ccc aag gag ttg 211 Leu Gy Thr Arg Phe Leu Pro Ala Thr Lys Thr Val Pro Lys G u Leu 30 ctg ccg gtt gtc gat acc cca ggt att gag ctg att gct gct gag gct 259 Leu Pro Val Val Asp Thr Pro Gy lie Gu Leu lie Ala Ala Gu Ala 45 gcc gaa ctt ggt gcg acc agg ctg gcg atc atc act gcg cca aac aaa 307 Ala u Leu y Ala Thr Arg Leu Ala lie lie Thr Ala Pro Asn Lys 60 gct ggg gta ctt gca cac ttt gag cgt tct tct gaa ttg gaa gaa acg 355 Ala y Val Leu Ala His Phe G u Arg Ser Ser G u Leu G u G u Thr 75 80 ctg atg gag cgt ggc aag act gac cag gtg gag ata atc cgc cgc gcc 403 Leu IVet u Arg y Lys Thr Asp n Val Gu Ile Ile Arg Arg Ala 95 100 gcc gat tta atc aag gca gtt cca gta acc cag gac aag ccg ctg ggg 451 Ala Asp Leu Ile Lys Ala Val Pro Val Thr G n Asp Lys Pro Leu y 105 110 115 cta ggt cat gct gtt ggt ttg gct gag tct gtg ttg gat gat gat gaa 499 Leu G y His Ala Val G y Leu Ala G u Ser Val Leu Asp Asp Asp G u 120 125 130 gat gtc gta gcg gtg atg ttg ccg cac 526 Asp Val Val Ala Val Net Leu Pro His 135 140 <210> 270 <211> 142 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 270 IVet Ser Leu Pro Ile Asp G u His Val Asn Ala Val Lys Thr Val Val 1 5 10 Val Pro Ala Ala Gy Leu y Thr Arg Phe Leu Pro Ala Thr Lys Thr 25 Val Pro Lys G u Leu Leu Pro Val Val Asp Thr Pro Gy Ile G u Leu 40 Ile Ala Ala G u Al a Al a u Leu G y Al a Thr Arg Leu Al a lie lie 55 Page 347 Gene Sequence 24 Thr Ala Pro Asn Lys Al a Gy Val Leu Ala His 70 75 Su Leu u u Thr Leu IVet u Arg y Lys 90 Ile Ile Arg Arg Ala Ala Asp Leu Ile Lys Al a 100 105 Asp Lys Pro Leu G y Leu G y His Ala Val G y 115 120 Leu Asp Asp Asp G u Asp Val Val Ala Val Ibet 130 135 <210> 271 <211> 1284 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1261) <223> RXA01262 <400> 271 tatactcgtc aagggccttc gataaaacaa agacaatttt aaacttgctg tatcaataaa acacgaaaag gaatactttt -2-06. txt Phe G u Arg Ser Ser Thr Asp G n Val G u Val Pro Val Thr G n 110 Leu Ala G u Ser Val 125 Leu Pro His 140 cccccgacgg gacaatctga gca Al a aat Asn gtt Val ct g Leu gct Al a gac Asp gag G u act Thr gct Al a ct c Leu aaa Lys gaa G u act Thr aaa Lys gag G u gt c Val cct Pr o 120 at t l Ie t at Tyr gtt Val cgt Ar g cct Pr o gca Al a aac Asn aag Lys ggt Sy ttt Phe gtt Val gca Al a tcg Ser caa G n gat Asp 75 ttt Phe gtt Val ttt Phe t ca Ser ggg Gy gtt Val cca Pr o aac Asn ttt Phe ttt Phe tct Ser aca Thr cct Pr o 140 t ca Ser at t I e gt c Val act Thr gtt Val act Thr gga G y 110 gaa G u ttc Phe aat Asn 15 gat Asp gat Asp gcc Al a at t SIle t cc Ser 95 t cc Ser ct a Leu gca Al a gaa G u agc Ser aca Thr gca Al a 80 agc Ser aca Thr cgc Ar g atg aaa att gcc gtc et Lys Ile Ala Val 1 gct ctc ctc tct aaa Ala Leu Leu Ser Lys gaa cga gtg aaa cta G u Arg Val Lys Leu gat ctc gaa gaa tat Asp Leu G u G u Tyr acg gac gcc gaa gcc Thr Asp Al a u Al a acg cca act aat tac Thr Pro Thr Asn Tyr gtt gag tcc gta att Val u Ser Val lie 100 atc gta att aaa tcg Ile Val Ile Lys Ser 115 att aag cat cca gaa Ile Lys His Pro u 130 ctg cgt gaa ggc cga gca ttc Leu Arg G u G y Arg Ala Phe 145 Page 348 Gene Sequence 24-2-06.txt t ac Tyr 150 ct g Leu cct Pr o ttt Phe gat Asp ggg G y 230 ttt Phe gcc Al a gca Al a t ca Ser gat Asp 310 gcc Al a act Thr t ac Tyr gt a Val aat ctc tac cca tcc Asn gaa Gu at t SIle aat Asn 200 t at Tyr ggg G y t at Tyr t at Tyr aag Lys 280 act Thr ttt Phe gga G y t ac Tyr gac Asp 360 gaa Gu Tyr ttt Phe 170 ctt Leu t at Tyr tct Ser gat Asp gga Gy 250 gat Asp cgt Ar g gtt Val tct Ser gaa Gu 330 tcg Ser at t SIle gtt Val Pr o 155 gcg Al a acg Thr ctt Leu gtt Val cca Pr o 235 t at Ty r gt c Val aag Lys gga Gy tct Ser 315 at t SIle aag Lys at t SIle t at Ty r Ser act Thr gac Asp gca Al a cga Ar g 220 cgt Ar g tgt Cys ccg Pr o gac Asp at t SIle 300 tct Ser gt a Val at c SIle gca Al a aca Thr 380 aga gtt gtc gtt ggt gat Arg Val Val Val y Asp 160 ctg tta gct gag ggg gca Leu Leu Al a u G y Al a 175 tca act gag gca gag gcg Ser Thr G u Ala G u Al a 190 ctg cga gtt gct ttt ttc Leu Arg Val Ala Phe Phe 205 210 agc ttg gat act aag cag Ser Leu Asp Thr Lys G n 225 att gga tct cat tac aat I le y Ser His Tyr Asn 240 ctt ccg aaa gat acg aaa Leu Pro Lys Asp Thr Lys 255 cag aat cta atc tct gca G n Asn Leu Ile Ser Al a 270 ttt att gca gag gat atc Phe Ile Ala G u Asp Ile 285 290 tac cgc ctt gta atg aag Tyr Arg Leu Val IVet Lys 305 att caa gga gtc atg aaa Ile n y Val et Lys 320 gta ttt gaa ccg aat ctc Val Phe G u Pro Asn Leu 335 ctt aat gac atc gaa gag Leu Asn Asp Ile G u G u 350 aat cgt cca acc gat gag Asn Arg Pro Thr Asp G u 365 370 cgt gat att ttc cag cgt Arg Asp Ile Phe G n Arg 385 cgc agt cct Arg Ser Pro 165 aaa gaa aag Lys G u Lys 180 att aaa tta I e Lys Leu 195 aac gaa ct g Asn G u Leu at t at t gaa lie lie G u aat cct t ca Asn Pro Ser 245 cag ctt ct c G n Leu Leu 260 gt a gt c caa Val Val G n 275 ct c agt aaa Leu Ser Lys t ct gga tca Ser G y Ser cga at t aag Arg Ile Lys 325 gga gaa gaa G y G u G u 340 ttt aag gat Phe Lys Asp 355 ctt tct gat Leu Ser Asp gac Asp 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1261 1284 taagtggaaa gaatcttttg ttg <210> 272 <211> 387 <212> PRT <213> Corynebacter i um gl ut ai cum Page 349 Gene Sequence 24-2-06.txt <400> 272 Net 1 Al a G u Asp Thr Thr Val SIle SIle G u 145 Gy Gy G u Phe Lys 225 Tyr Thr Ser Asp bet 305 bet Asn SIle Leu Val G u Al a Thr Ser SIle 115 Hi s Ar g Ar g Lys SIle 195 Asn SIle Asn G n Val 275 Leu Ser Ar g Gy Val 5 Lys Leu Tyr Al a Tyr I le Ser G u Phe Pr o 165 Lys Leu Leu G u Ser 245 Leu G n Lys Ser Lys 325 G u Al a Asn Val Leu Al a 70 Asp G u Thr Al a Ty r 150 Leu Pr o Phe Asp Gy 230 Phe Al a Al a Ser Asp 310 Al a Thr G y Leu G y Hi s Lys Val Sn G u Phe 40 Ser Thr Lys Tyr Lys G y Pro u Ser Ile Val Leu 105 li e Pro Val 120 Ser Ile Ile 135 Asp Asn Leu Sy u u Pro Ile Leu 185 Ser Asn Thr 200 Thr Tyr Al a 215 Val G y Leu G y Tyr G y Asn Tyr Lys 265 Asn Lys Thr 280 Pro Thr Val 295 Asn Phe Arg Lys G y Ile Phe Tyr Asn 345 Tyr 10 Val Ar g Pr o Al a Asn 90 Lys Gy Phe Tyr Phe 170 Leu Tyr Ser Asp Gy 250 Asp Ar g Val Ser G u 330 Val G y Leu Ser Al a Ser G n Asp 75 Phe Val Phe Ser Pr o 155 Al a Thr Leu Val Pr o 235 Tyr Val Lys Gy Ser 315 I le Asp I e Leu SIle Asp Pr o Ser 125 G u Ar g Leu Ser Leu 205 Ser SIle Leu G n Phe 285 Tyr SIle Val Asn Ala Asp u Asp Ser Al a Thr I e Al a Ser Ser Ser Thr Leu Arg Leu Arg Val Val 160 Al a u 175 G u Al a Val Ala Asp Thr Ser His 240 Lys Asp 255 Leu II e Al a u Leu Val G y Val 320 G u Pro 335 Ser Lys Ile Leu Asn Asp Ile 350 Page 350 Gene Sequence 24- 2- 06. txt Ou u Phe Lys Asp Tyr Cys Asp lie lie lie Ala Asn Arg Pro Thr 355 360 365 Asp G u Leu Ser Asp Val Pro G u Lys Val Tyr Thr Arg Asp Ile Phe 370 375 380 Sn Arg Asp 385 <210> 273 <211> 1209 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1186) <223> RXA01377 <400> 273 tgaaatggat ttgctgcggc cccggaatta cccttttcgc ggccgtcatc aaatttgtac ccccttaaag acaccctaaa cacgagtgaa ataggaacac aac Asn cga Ar g gct Al a ggc G y gag G u gt c Val t ac Tyr ct c Leu gac Asp ttt Phe 150 aaa Lys cgc Ar g cac Hi s aca Thr t ac Tyr gag Gu aag Lys ggt Gy 120 gat Asp tgc Cys gtt Val ct g Leu ttc Phe gt c Val gga G y cag G n cgt Ar g gat Asp acc Thr ccc Pr o gct Al a gt c Val acc Thr ct g Leu ggc G y ttg Leu gat Asp aac Asn cat Hi s 140 gat Asp at c l Ie act Thr ctt Leu acg Thr gaa G u act Thr gcg Al a 110 at t SIle gt g Val gat Asp ttg Leu 15 cca Pr o ttg Leu t ca Ser at g Met ggt Gy 95 at t l Ie ct g Leu cgc Ar g ggt Gy gt c Val aag Lys gcc Al a ttc Phe ggc G y 80 ggt Gy gt g Val gac Asp gt a Val cgc Ar g 160 atg act tta act gac IVet Thr Leu Thr Asp 1 ggt ggc aaa ggt acc G y G y Lys G y Thr cca atg ctg cca act Pro IVet Leu Pro Thr cgc atc aag gcc gca Arg lie Lys Ala Ala aaa gct gaa gtc ttc Lys Ala G u Val Phe ttg gaa att gaa tat Leu G u lie G u Tyr ggc atc cga aac gtc Gy lie Arg Asn Val 100 ttc aac ggc gat gtg Phe Asn G y Asp Val 115 acc cac cgc gaa aag Thr His Arg G u Lys 130 gct aac cct cgt gcg Ala Asn Pro Arg Ala 145 gtc agc gaa ttc ctt Val Ser G u Phe Leu 165 gaa aag acc gaa gat cca cca acc gat cag atc aac gcc ggc tgc tac Page 351 Gene Sequence 24-2-06. t xt Su Lys Thr u Asp Pro Pro Thr Asp n Ile Asn Ala y Cys Tyr 170 175 180 gtg ttc aag aag gaa ctc atc gag cag atc ccg gca ggc cga gca gtt 691 Val Phe Lys Lys G u Leu lie G u n Ile Pro Ala Gy Arg Ala Val 185 190 195 tcc gtc gag cgc gaa acc ttc cct cag ctg ttg gaa gaa ggc aag cga 739 Ser Val G u Arg G u Thr Phe Pro G n Leu Leu G u G u y Lys Arg 200 205 210 gtc ttc ggc cac gtc gac gct tcc tac tgg cgc gac atg ggc acc cca 787 Val Phe G y Hi s Val Asp Al a Ser Tyr Trp Arg Asp IVbt G y Thr Pro 215 220 225 agc gac ttc gtc cgc ggc tcg gct gac ctg gtc cgc ggc att gcg tac 835 Ser Asp Phe Val Arg G y Ser Ala Asp Leu Val Arg y Ile Ala Tyr 230 235 240 245 tcc cca ttg ctc gaa ggc aaa aca gga gag tcg ctt gtc gac gcc tcc 883 Ser Pro Leu Leu G u G y Lys Thr Gy u Ser Leu Val Asp Ala Ser 250 255 260 gcc ggc gtt cgc gac ggc gtc ctg ctg ctc ggc gga acc gta gtc ggc 931 Ala y Val Arg Asp G y Val Leu Leu Leu Gy y Thr Val Val y 265 270 275 cgc ggc act gag atc ggt gcc ggc tgc cgc gtt gac aac act gtt att 979 Arg y Thr u lie y Ala G y Cys Arg Val Asp Asn Thr Val lie 280 285 290 ttc gac ggc gtc acc att gaa cca ggt gcg gtc att gaa aat tcc atc 1027 Phe Asp y Val Thr Ile u Pro G y Ala Val lie u Asn Ser lie 295 300 305 att t cc tcg gga gca cgc atc ggt gct aat gcg cac atc tcc ggt tgc 1075 Ile Ser Ser G y Ala Arg lie y Ala Asn Ala His lie Ser G y Cys 310 315 320 325 atc att ggc gag ggc gca cag gtgtggt gct cgg tgt gaa ctc aac gca 1123 lle le Gy Gu G y Ala Gn Val Gy Ala Arg Cys u Leu Asn Ala 330 335 340 ggg atg cgc gtc ttc cca ggc gtt gtg atc cca gac agc gga att cgt 1171 Gy IVbt Arg Val Phe Pro Gy Val Val Ile Pro Asp Ser Gy IIe Arg 345 350 355 ttt tcg tct gat cag taggcatttt tagccctttt gga 1209 Phe Ser Ser Asp G n 360 <210> 274 <211> 362 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 274 IVbt Thr Leu Thr Asp Asn Ser Lys Asn Val Asp Al a Val Ile Leu Val 1 5 10 Gy Gy Lys Gy Thr Arg Leu Arg Pro Leu Thr Val Asn Thr Pro Lys 25 Pro IVbt Leu Pro Thr Al a Gy His Pro Phe Leu Thr His Leu Leu Ala 40 Arg lie Lys Ala Ala G y Ile Thr His Val Val Leu y Thr Ser Phe Page 352 Gene Sequence 24-2-06.txt G y Ser G u Vbet Lys Leu Gy Phe Thr Al a 145 Val Asn Al a G u Asp 225 Ar g Leu Gy Asp SIle 305 Hi s Cys Asp G u I e Ar g Gy 115 Ar g Pr o G u Gy Ar g 195 Gy Gy I e Asp Val 275 Thr Asn Ser Leu Gy 355 Phe Ty r Val Val Lys Al a Leu 165 Tyr Val Ar g Pr o Tyr 245 Ser Gy I e I e Cys 325 Al a Ar g G u Val Asp Ser Al a 135 Gy Lys Phe Val Phe 215 Asp Pr o Gy Gy Asp 295 Ser SIle bet Ser Tyr Phe Gy Asp 75 Su Asp n Pro 90 Lys Leu Arg His 105 G y Ala Asp Leu 120 Asp Leu Thr IVet Cys Val Pro Thr 155 Thr G u Asp Pro 170 Lys Lys G u Leu 185 G u Arg G u Thr 200 G y His Val Asp Phe Val Arg y 235 Leu Leu G u G y 250 Val Arg Asp y 265 Thr G u Ile G y 280 Sy Val Thr lle Ser y Ala Arg 315 Sy u y Al a 330 Arg Val Phe Pro 345 Ser Asp G n 360 Leu Asp Asn Hi s 140 Asp Pr o SIle Phe Al a 220 Ser Lys Val Al a G u 300 I le G n Sy Gy Thr Ser 125 Leu G u Thr G u Pr o 205 Ser Al a Thr Leu Sy 285 Pr o Sy Val Val Thr Al a 110 SIle Val Asp Asp G n 190 G n Tyr Asp Sy Leu 270 Cys Sy Al a Sy Val 350 Gy l le Leu Ar g Sy G n 175 I le Leu Tr p Leu G u 255 Leu Ar g Al a Asn Al a 335 I le Sy Gy Val Asp Val Ar g 160 I e Pr o Leu Ar g Val 240 Ser Sy Val Val Al a 320 Ar g Pr o <210> 275 <211> 1350 <212> DNA <213> <220> <221> <222> Cor ynebact eri um gl ut arri cum CDS (1327) Page 353 Gene Sequence 24-2-06.txt <223> RXA02063 <400> 275 accgaaatgg gggcattaaa aggggctatc attcggaccc ttgttaccca gctttcatgc gggatagtta ttttgccttt caaaacgatg tttagacaat atg gtt aag ggt gtg 111 let Val Lys G y Val aag Lys aaa Lys ttc Phe aac Asn t ca Ser ggg Gy tgg Tr p tct Ser t at Tyr cgc Ar g 150 gcg Al a ttg Leu act Thr cag G n ggc G y 230 aga Ar g ctt Leu gga G y gga G y gat Asp t ac Tyr act Thr gag G u 120 at g Net gt g Val ggt Sy aag Lys gcg Al a 200 ct g Leu gac Asp aat Asn ccg Pr o t ac Tyr ct c Leu cat Hi s gct Al a t cc Ser ccg Pr o cca Pr o gt g Val at c SIle 170 gct Al a at g et gat Asp at t SIle ct a Leu acg Thr ttg Leu at c SIle t ca Ser gtt Val gat Asp t at Tyr cag G n 140 ggt G y t cc Ser cct Pr o aac Asn gaa G u 220 t ac Tyr gca Al a gag G u at c I e 45 gcg Al a ttg Leu cct Pr o gca Al a gt c Val 125 at g Net at t SIle gat Asp ccg Pr o t ac Tyr 205 aat Asn ttc Phe at c l Ie gac Asp gac Asp gt a Val tcg Ser gcg Al a at t l Ie 110 at c SIle ct a Leu cgc Ar g gt c Val gga G y 190 at t SIle aac Asn gt c Val gtt Val 15 cga Ar g ttt Phe ct g Leu tgg Tr p cag G n 95 ttg Leu gtt Val gat Asp gtt Val gac Asp 175 acc Thr ttc Phe gag G u tct Ser ct c Leu gct Al a gtt Val act Thr aac Asn 80 cag G n cag G n ttc Phe gag G u cca Pr o 160 ggc G y cct Pr o acc Thr aac Asn cgc Ar g 240 1 gca Al a aag Lys ttg Leu cag G n gt g Val cgc Ar g tct Ser ggc G y cac Hi s 145 cgt Ar g aac Asn gat Asp act Thr agt Ser 225 aat Asn ggt G y cct Pr o tcg Ser t ac Tyr tct Ser ct g Leu ct g Leu gcg Al a 130 at t SIle gag G u at a SIle gat Asp gaa G u 210 gat Asp gat Asp ggc G y gcg Al a aac Asn aag Lys ggt G y ggc G y aac Asn 115 gac Asp gca Al a gaa G u acc Thr cct Pr o 195 gcc Al a cat Hi s gcg Al a gag G u gt c Val ct g Leu tcg Ser cca Pr o aag Lys 100 ttg Leu cac Hi s tct Ser gca Al a gag G u 180 gac Asp ct g Leu gac Asp cat Hi s ggc G y cca Pr o gtg Val cat Hi s acg Thr cga Ar g at c SIe gtg Val ggt G y act Thr 165 ttc Phe at g let at c SIe at g bet gtt Val 245 Page 354 Gene Sequence 24-2-06.txt t at Tyr ggc G y at g et tgg Tr p gtt Val 310 at t SIle gt c Val cgc Ar g gt g Val gcg Al a 390 aac Asn ttc Phe tgg Tr p ct g Leu 280 at c SIle ggc G y gct Al a gag Gu ggt Gy 360 gt c Val cgc Ar g gt a Val t cc gga aac Ser Gy Asn 250 cgc gac gt c Arg Asp Val 265 att tcc gtg Ile Ser Val cac acg acc Hi s Thr Thr ggt atc gcg G y I le Al a 315 ggg act gt t G y Thr Val 330 ggc gca acg G y Al a Thr 345 aag ggt gct Lys G y Al a cgc gac gga Arg Asp G y ttc aag gtg Phe Lys Val 395 at t SIle ggt G y cac Hi s tct Ser 300 cag G n cgc Ar g gt g Val gtt Val gag G u 380 agc Ser gtt Val acc Thr cca Pr o 285 gaa G u tcg Ser aac Asn gaa G u gt c Val 365 ct c Leu gcc Al a cct ggc gca act Pro G y Ala Thr 255 att gat gcg ttc I e Asp Al a Phe 270 atc ttc aat ctg Ile Phe Asn Leu ggt aac ttg cct Sy Asn Leu Pro 305 tcg atg gtg tct Ser Met Val Ser 320 t cc gtg ctg t cc Ser Val Leu Ser 335 ggt gca gtg ctg Sy Ala Val Leu 350 cgc cat gcg at t Arg His Ala lle atc ggt gtc gac lie y Val Asp 385 ggc ggc gtc gtg Sy y Val Val gag G u t ac Tyr t at Tyr 290 ccg Pr o t ca Ser aac Asn at g Net ct g Leu 370 caa G n gtt Val cgt gac aag Arg Asp Lys 260 gag t gc cac G u Cys His 275 aac t ct gag Asn Ser G u gct aag ttc Ala Lys Phe ggt t cc at c G y Ser lie 325 aac gtt gtc Asn Val Val 340 cca ggc gt g Pro G y Val 355 gac aag aac Asp Lys Asn gtg cgc gat Val Arg Asp gtc ggt aag Val G y Lys 405 883 931 979 1027 1075 1123 1171 1219 1267 1315 1350 gt c Val taaacgggaa agggacctta aaa <210> 276 <211> 409 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 276 Iet Val Lys y Val Lys y Arg Pro 1 5 Ala G y G y G u G y Lys Arg Leu Phe Lys Pro Ala Val Pro Phe Gy Gy Thr 40 Leu Ser Asn Leu Val Asn Ser G y Phe G n Tyr Lys Ser His Ser Leu Asp Arg 70 Val Ser y Pro Thr y n Tyr lie Asn Val I 10 Pro Leu Tyr Arg I Leu Lys His Il e Al a Ser Page 355 Al a G u SIle Al a Leu Pr o Val Ar g Phe Leu Tr p G n ene Sequence 24- 2- 06. txt Ser Al aAsp Al a Ar g Ser G31y Hi s 145 Ar g As n As p Th r Ser 225 As n Th r Ph e Le u Pr o 305 Ser Ser Le u I Ie As p 385 ValI G31y As n 115 As p Al a (31u Th r Pr o 1 95 Al a Hi s Al a Ar g (31u 275 As n Al a G31y As n Pr o 355 As p ValI ValI Ar g I Ie ValI G31y Th r 1 65 Ph e vb t I Ie vb t ValI 245 Ly s Hi s (31u Ph e I Ie 325 ValI ValI As n As p Ly s 405 Tr p Ser Ty r Ar g 150 Al a Le u Th r O31n G31y 230 Ty r G31y vb t Tr p ValI 310 li1e ValI Ar g ValI Al a 390 As n Ph e As p Ar g 135 Al a Ph e (31u Ty r Al a 215 G31y As p Ty r As p Pr a 295 Ar g Ser O31u I Ie ValI 375 O31n O31n Thr 03 y 1 (3 u Lys 120 l'b t As p Val Ser 31y cys Lys Pr o 1 Al a Ser 200 Leu Lys As p II e Phe Ser Tr p Ar g 265 Leu I I e 280 1I e Hi s G31YG(1Y Al a 03 y (31u (1y 345 O3 y Lys 360 Val Ar g Ar g Phe Val Val Pr o Pr a ValI I Ie 1 70 Al a vb t As p I Ie G31y 250 As p Ser Th r I Ie Th r 330 Al a G31y As p Ly s As p Ser Al a 1 O31n As p G31y As p Pr a 235 As n ValI ValI Th r Al a 315 ValI Th r Al a G31y ValI 395 ValI 125 vb t I Ie As p Pr a Ty r 205 As n Ph e ValI Th r Pr a 285 O31u Ser As n O31u ValI 365 Le u Al a Le u ValI As p ValI As p 1 Th r Ph e O31u Ser G31y 255 As p Ph e As n vb t ValI 335 Al a Hi s G31y G31y <210O> 277 <21 1> 903 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn Page 356 Gene Sequence 24-2-06.txt <220> <221> CDS <222> (101)..(880) <223> RXN00014 <400> 277 catcaaagtg accgccggcg gcgtcgaatg gtccgttgca tgagatctcc gaaactttaa gcgcactaga ctaacaacac ggaaacgcgg aagcagttag gac Asp acc Thr gac Asp cgt Ar g gaa G u ccc Pr o acc Thr t cc Ser gca Al a cag G n 150 ggc Gy acc Thr gt c Val tgg Tr p tta Leu gac Asp act Thr aaa Lys ggt Gy gac Asp at c SIle ccc Pr o 120 cgc Ar g t cc Ser gcc Al a tgg Tr p gaa Gu 200 ctt Leu tta Leu ttc Phe gt c Val gcc Al a gac Asp acc Thr ct g Leu ct g Leu ttc Phe ctt Leu cga Ar g 170 ct c Leu gcc Al a ccc Pr o gcc Al a gaa Gu agc Ser acc Thr gt a Val 75 aaa Lys ct c Leu gct Al a aac Asn gac Asp 155 gat Asp cgc Ar g gt c Val ct g Leu ct c Leu gcc Al a gat Asp gcc Al a gaa G u aac Asn gac Asp agg Ar g ggc Gy 140 gac Asp ttg Leu ggc Gy gat Asp t cc Ser 220 ctt Leu gac Asp gac Asp ccc Pr o agc Ser gt c Val ggc Gy 110 tgg Tr p t cc Ser t cc Ser gat Asp ggc Gy 190 gcc Al a ct g Leu gcc Al a 15 ct g Leu ct g Leu gcc Al a ggc Gy cgc Ar g 95 aaa Lys tgg Tr p cca Pr o ct c Leu cag G n 175 gac Asp gct Al a gt c Val gaa G u gaa G u gcg Al a gac Asp cgc Ar g 80 ggc Gy ccc Pr o gca Al a cgc Ar g t cc Ser 160 ttc Phe ttc Phe gaa G u acc Thr at g Met 1 ctt Leu gt a Val acc Thr t cc Ser cag G n gt c Val gt c Val t cc Ser aaa Lys 145 ttc Phe gt c Val ttc Phe cca Pr o agc aaa tat Ser Lys Tyr gcc Al a t cc Ser gaa G u at c SIle tgg Tr p ccc Pr o gca Al a gaa G u 130 ct g Leu t cc Ser t cc Ser t cc Ser gaa 10u 210 gat Asp t cc Ser gaa G u ct c Leu at c SIle gt a Val ggt Gy 115 ggg Gy t cc Ser t cc Ser ct a Leu t ac Tyr 195 gt c Val t cc Ser aag Lys gca Al a ggt Gy at c SIle tgg Tr p 100 gt c Val gcc Al a gt g Val ct c Leu act Thr 180 tgc Cys agc Ser gca Al a at c SIe cca Pr o ct c Leu gaa Gu gac Asp gca Al a at c SIe ggc Gy t cc Ser t cc Ser 165 gat Asp ct c Leu ct c Leu gaa gcc gga gga aag Su Ala Gy y Lys 225 Page 357 ene Sequence 24- 2- 06. t xt t tc acc t ca ct g gct ggc gt c gat gga cca cac ggt ggc gat gca gt a Phe Thr Ser Leu Ala G31y Val As p G31y Pro His Gy Gy Asp Ala Val 230 235 240 245 gcc acc aac ggc at c ct g cac gat gag acg ct g gat cgt t t a aaa Ala Th r As n 3 y Ilie Leu Hi s Asp (3u Th r Leu Asp Arg Leu Lys 250 255 260 t agact cccg ggtt t tgct t ggt <210> 278 <211> 260 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 278 I'bt Ser Lys Tyr A a Asp Le u ValI Thr Ser O31n ValI ValI Ser Ly s 145 Ph e ValI Ph e Pr o (31u 225 (31y As p Ser (31u Le u I Ie ValI G31y 115 G31y Ser Ser Le u Tyr 1 95 ValI G31y As p Ser I Ie Lys Pr o A a Leu (31y (1u I I e As p Tr p Ala 1 00 Val I Ie A a 03 y Val Ser Leu Ser 1 65 Thr As p 1 80 Cys Leu Ser Leu (3 y Lys A a Val 245 Thr As p Ar g (31u 70 Pr o Thr Ser A a O31n 150 G31y Thr ValI Tr p Ph e 230 As p Le u vb t O31u Ph e I Ie Le u A a Tr p 135 ValI Tr p Thr A a As p 215 Thr Le u As p Thr 40 Ly s G31y As p I Ie Pr o 120 Ar g Ser A a Tr p O31u 200 Le u Ser A a Ar g Pr a I Ie G31y G31y A a 1 A a Thr Ly s O31u Ar g 1 G31y A a Le u Le u 10 Ph e ValI A a As p Thr 90 Le u Le u Ph e Le u Ar g 1 70 Le u A a Pr a A a I Ie 250 A a O31u Ser Thr ValI Ly s Le u A a As n As p 1 As p Ar g ValI Le u G31y 235 Le u Le u A a As p A a O31u As n As p Ar g G31y 140 As p Le u G31y As p Ser 220 ValI Hi s O31u Ser A a Ar g Ph e Tyr As n Ar g 125 Ser A a Ar g Tyr I Ie 205 li1e As p As p A a Le u Le u A a G31y Ar g Ly s Tr p Pr a Le u O31n 1 As p A a ValI Pr a Th r 255 A a Th r As n (3 y Asp Arg Leu Page 358 Gene Sequence 24-2-06.txt <210> 279 <211> 903 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(880) <223> FRXA00014 <400> 279 catcaaagtg accgccggcg gcgtcgaatg gtccgttgca tgagatctcc gaaactttaa gcgcactaga ctagcaacac ggaaacgcgg aagcagttag agc aaa tat Ser Lys Tyr gac Asp acc Thr gac Asp cgc Ar g gaa Gu ccc Pr o acc Thr t cc Ser gca Al a cag G n 150 ggc Gy acc Thr gt c Val tta Leu gac Asp act Thr aaa Lys ggt Gy gac Asp at c SIle ccc Pr o 120 cgc Ar g t cc Ser gcc Al a tgg Tr p gaa Gu tta Leu ttc Phe gt c Val gcc Al a gac Asp acc Thr ct g Leu ct g Leu ttc Phe ctt Leu cga Ar g 170 ct c Leu gcc Al a gcc Al a gaa G u agc Ser acc Thr gt a Val 75 aaa Lys ct c Leu gct Al a aac Asn gac Asp 155 gat Asp cgc Ar g gt c Val ct c Leu gcc Al a gat Asp gcc Al a gaa Gu aac Asn gac Asp agg Ar g ggc Gy 140 gac Asp ttg Leu ggc Gy gat Asp ctc gcc gaa Leu Al a G u 15 gac ctg gaa Asp Leu G u gac ctg gcg Asp Leu Al a ccc gcc gac Pro Al a Asp agc ggc cgc Ser G y Arg 80 gtc cgc ggc Val Arg y 95 ggc aaa ccc G y Lys Pro 110 tgg tgg gca Trp Trp Al a t cc cca cgc Ser Pro Ar g t cc ctc t cc Ser Leu Ser 160 gat cag ttc Asp G n Phe 175 ggc gac ttc G y Asp Phe 190 gcc gct gaa Al a Al a G u gcc Al a t cc Ser gaa G u at c SIle tgg Tr p ccc Pr o gca Al a gaa G u 130 ct g Leu t cc Ser t cc Ser t cc Ser gat Asp t cc Ser gaa G u ct c Leu at c SIle gt a Val ggt Gy 115 ggg Gy t cc Ser t cc Ser ct a Leu t ac Tyr 195 t cc Ser aag Lys gca Al a ggt Gy at c SIle tgg Tr p 100 gt c Val gcc Al a gt g Val ct c Leu act Thr 180 tgc Cys gca Al a at c SIe cca Pr o ct c Leu gaa G u gac Asp gca Al a at c SIe ggc Gy t cc Ser t cc Ser 165 gat Asp ct c Leu cca gaa gtc agc ctc Pro G u Val Ser Leu Page 359 Gene Sequence 24-2-06.txt 200 205 210 tgg gat ctt gct ccc ctg tcc atc ctg gtc acc gaa gcc gga gga Trp Asp Leu Ala Pro Leu Ser Ile Leu Val Thr G u Ala Gy y 215 220 225 ttc acc tca ctg gct ggc gtc gat gga cca cac ggt ggc gat gca Phe Thr Ser Leu Ala G y Val Asp Gy Pro His Gy Gy Asp Ala 230 235 240 gcc acc aac ggc at ct g cac gat gag acg ctg gat cgt tta aaa Ala Thr Asn G y IIe Leu His Asp G u Thr Leu Asp Arg Leu Lys 250 255 260 tagactcccg ggttttgctt ggt <210> 280 <211> 260 <212> PRT <213> Corynebacter i um gl utam cum <400> 280 IVet Ser Lys Tyr Ala Asp Asp 1 5 Leu Ala Asp Ser lie Thr Leu Val Ser Ser Lys Pro Asp IVet Thr G u G u Ala Leu Arg G u 55 Ser Ile Leu Gy Gu Gu Phe 70 G n Trp Ile Ile Asp Pro Ile Val Pro Val Trp Ala Thr Leu 100 Val Ala y Val lie Ser Ala 115 Ser Gu Gy Ala Gy Ala Trp 130 135 Lys Leu Ser Val Ser G n Val 145 150 Phe Ser Ser Leu Ser G y Trp 165 Val Ser Leu Thr Asp Thr Thr 180 Phe Ser Tyr Cys Leu Val Al a 195 Pro G u Val Ser Leu Trp Asp 210 215 G u Al a G y G y Lys Phe Thr 225 230 Al a Ar g 25 Pr o SIle Sy Sy Al a 105 Al a Thr Lys G u Ar g 185 Sy Al a Leu Leu 10 Phe Val Al a Asp Thr 90 Leu Leu Phe Leu Ar g 170 Leu Al a Pr o Al a G u Ser Thr Val Lys Leu Al a Asn Asp 155 Asp Ar g Val Leu Leu Al a Asp Al a G u Asn Asp Ar g Sy 140 Asp Leu Sy Asp Ser 220 G u Ser Al a Ar g Phe Tyr Asn Ar g 125 Ser Al a Ar g Tyr SIle 205 I le Leu Asp Asp Pr o Ser Val Sy 110 Tr p Ser Ser Asp Sy 190 Al a Leu Al a Leu Leu Al a Sy Ar g Lys Tr p Pr o Leu G n 175 Asp Al a Val Al a G y 235 Val Asp G y Pro His 240 Page 360 Gene Sequence 24-2-06.txt G y y Asp Ala Val Ala Thr Asn Gy IIe Leu His Asp Gu Thr Leu 245 250 255 Asp Arg Leu Lys 260 <210> <211> <212> <213> <220> <221> <222> <223> 281 978 DNA Cor ynebact eri um gl ut am cum CDS (955) RXA01570 <400> 281 cactgaactc cgttttggat ctcggcaaaa tcgaagccac cggatttagc gcaccgacct ggcagacccg cctcaacgac tacctcaagg aactctcaaa aaa ggc atc Lys G y I I e etc gca ggt ggc Leu Al a G y G y t cc Ser ggc acc cgg ctc G y Thr Arg Leu ccc atc acc aag Pro Ile Thr Lys ggc atc G y le tcc aag caa Ser Lys G n ctg acc acg Leu Thr Thr atg ccg att tac IVet Pro Ile Tyr gac Asp aaa ccc atg gtc Lys Pro Met Val t ac t ac cca Tyr Tyr Pro att atc acc Ile Ile Thr ctc att cag gcc Leu i e G n Al a atc aaa gac at c Ile Lys Asp Ile ct g Leu acc cct Thr Pro gaa gac agc gcc G u Asp Ser Al a ttt gaa cgc ttg Phe G u Arg Leu ggc gac ggc tcc G y Asp G y Ser tcc tgg ggc atc aac ctc acc tac gcc gtc caa Ser Trp y lie Asn Leu Thr Tyr Ala Val G n 75 ccc tcc ccc gac Pro Ser Pro Asp cta gcc caa gca Leu Ala G n Ala ttc Phe atc atc ggc gag lie lie Gy u gaa Su 95 ttc atc ggt gac Phe Ile G y Asp gac gac Asp Asp 100 gtc gcg ttg Val Ala Leu cac gca ct a His Ala Leu 120 ctt ggc gat aac Leu G y Asp Asn at c SIle 110 ttc gac ggc gca Phe Asp G y Al a caa ctt ggc G n Leu G y 115 gtc ttt gct Val Phe Al a aag cag tgc tcc Lys G n Cys Ser ccc gac ggt ggc Pro Asp G y G y at t SIle 130 tat gag Tyr G u 135 gtc tcc gat cct Val Ser Asp Pro gag 10u 140 cgt tat ggc gtg Arg Tyr y Val gaa ttt gat gct G u Phe Asp Ala gct Al a 150 aat aag gcg gtg Asn Lys Ala Val att gaa gaa aag Ile u u Lys ccc Pr o 160 acc gcg cca aaa Thr Ala Pro Lys aac ttt gcc gtg Asn Phe Ala Val gt a Val 170 gga cta tat ttc G y Leu Tyr Phe gac aat cgc gtg Asp Asn Arg Val gtg gac Val Asp 180 Page 361 at c SIle t cc Ser ct g Leu t cc Ser 230 at c SIle gct Al a gga Gy aag Lys aac Asn 200 cgt Ar g gcg Al a gga Gy gaa G u t ac Tyr 280 at c SIle gcc Al a gat Asp t cc Ser ccc Pr o 250 aca Thr ct g Leu aag Lys tac Tyr gt c Val t at Tyr 235 gaa G u gt g Val aga Ar g cct Pr o ct c Leu tgg Tr p 220 gtt Val gt c Val ctt Leu gct Al a Gene Sequence 24- 2- 06. txt tcc tcg cgt ggc gaa ctg gaa atc Ser Ser Arg Gy u Leu u lie 190 195 cag caa ggt gct tta act gtg cag Sn G n G y Al a Leu Thr Val G n 205 210 tta gat acc ggc aca atc gat tcc Leu Asp Thr y Thr Ile Asp Ser 225 gag gtc ctg caa aaa cgt acc ggc G u Val Leu G n Lys Arg Thr y 240 gct gcg tac cgc gaa ggt ttc atc Ala Ala Tyr Arg u y Phe Ile 255 260 ggt gag gaa ctg aag aaa tca ggc G y G u G u Leu Lys Lys Ser G y 270 275 ttg taatttacgg tgtggttgtg gag Leu 285 <210> 282 <211> 285 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 282 Val Lys Oy lie lie Leu Ala Gy y 1 5 I e Thr Lys y Ile Ser Lys n Leu 25 IVet Val Tyr Tyr Pro Leu Thr Thr Leu 40 Ile Leu Ile Ile Thr Thr Pro u Asp 55 Leu y Asp y Ser Ser Trp y lie 70 Pro Ser Pro Asp y Leu Ala G n Ala I e y Asp Asp Asp Val Ala Leu Val 100 105 G y Al a G n Leu G y Hi s Al a Leu Lys 115 120 Sy I e Val Phe Ala Tyr G u Val Ser 130 135 Val G u Phe Asp Ala Al a Asn Lys Ala 145 150 Thr Ala Pro Lys Ser Asn Phe Ala Val 165 Sy Pr o G n Al a Leu 75 l le Sy Cys Pr o Ser 155 Thr SIle Al a Ser Thr SIle Asp Ser G u 140 I le Ar g Tyr Sy Phe Tyr Sy Asn Asn 125 Ar g G u Leu Asp I le G u Al a G u SIle 110 Pr o Tyr G u Tyr Pro Lys Pro Lys Asp Arg Leu Val G n G u Phe Phe Asp Asp y G y Val Lys Pro 160 Tyr Asp 175 Val G y Leu Tyr Phe 170 Page 362 As n (31u Le u Thr 225 Ly s (31u Ly s ValI (31u 1 95 ValI As p Thr Ph e Ser 275 As p Thr Ar g vb t As n 245 Thr Tyr I Ie Ser Le u Ser 230 li1e Al a (31y Al a ValI As p 215 Ou le Gu As n ene Sequence 24- 2- 06. txt Lys Ser Ilie Lys Pro Ser Ser 185 190 Asn Asp Al a Tyr Leu G3 n G3 n 200 205 Ar g (31y Asp Val Tr p Leu Asp 220 Al a Ser Ser Tyr Val (31u Val 235 (31y Ser Pr o (31u Val Al a Al a 250 (31u Leu Thr Val Leu G31y (31u 265 270 Tyr Leu Leu Arg Ala Leu 280 285 Ar g G31y Thr Le u Tyr 255 (31u <210O> 283 <21 1> 891 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ari cumn <220> <221 ODS <222> (868) <223> RXA02666 <400> 283 gctcggcgac gaggaagaga agaaggacgc attcgacgac t ct tgacgat ct gagct tcg acgacgaaga t tagacgccc t tcgacgat t ccgacgt gga at c G31y t ta Le u at c I Ie ct g Le u ct c Le u c ag (31n gt c ValI CCC Pr o cgc Ar g at c I Ie aaa Ly s c ac Hi s at t I Ie gca Al a CCC Pr o ct c Le u gt c ValI ggt G31y ggC G31y ct t Le u ct c Le u aaa Ly s caa O31n ag c Ser ct t Le u 75 ggg G31y gac As p ct c Le u gca Al a gcc Al a CCC Pr o tt g Le u gag O31u g ac As p gcg Al a tt c Ph e at g vb t 45 g ac As p aac As n cgc Ar g gcc Al a gcg Al a gt c ValI ct c Le u at g vb t g ac As p gcg Al a ac c Thr 110 gcg Al a 15 ac g Thr ac c Thr g aa O31u ccc Pr a g ac As p 95 ccc Pr o ggg G31y tt g Le u t cc Ser ac c Thr g aa O31u 80 t cg Ser g at As p at g vb t 1 cgc Ar g cgt Ar g g aa O31u t ac Tyr ggg G31y gt c ValI gca Al a gga G31y g aa O31u ag c Ser gcc Al a gt a ValI t gg Tr p at t I Ie ac c Thr cgc Ar g gt c ValI cgc Ar g cgc Ar g gca Al a gt c ValI 115 cgc Ar g ac a Thr g ac As p g at As p gt a ValI ggc G31y 1 00 tt a Le u t cg t ct aca Ser Ser Thr cga Ar g ct c Le u ct t Le u g aa O31u tt g Le u cgg Ar g ct t Le u at c I Ie cac gac agc gcc cga gcg ctc aca cca ccc ggc atg att gcg cgc gtg Page 363 Hi s Asp Ser 120 Ala Arg Ala Leu Gene Sequence 24- 2- 06. txt Thr Pro Pro Gy Met Ile Ala Arg Val 125 130 gtg cgc aaa gtc cac gaa ggc gcc acc gca gtc Val Arg Lys Val His G u Gy Ala Thr Ala Val 135 140 gta tcg gac acc atc aaa cga gtg tcc cct gat Val Ser Asp Thr lie Lys Arg Val Ser Pro Asp 150 155 160 gac aca ccc aac cgt gca gaa ctt cgc gcc gtc Asp Thr Pro Asn Arg Al a G u Leu Arg Ala Val 170 175 ttc ctg ctg tcc gaa ctt gtt gca gcg aat gag Phe Leu Leu Ser G u Leu Val Ala Ala Asn G u 185 190 ccc aac cca ggc ttc atc cca acc gat gac gcc Pro Asn Pro y Phe Ile Pro Thr Asp Asp Al a 200 205 tac ggc gca gat gta gtc tgc gta caa ggc gac Tyr y Al a Asp Val Val Cys Val G n Gy Asp 215 220 gta aca acc ccc att gat atg atg ctg gca caa Val Thr Thr Pro lie Asp IVet IVet Leu Ala G n 230 235 240 gcc gaa ccc aca ata ttt gag gta cca ggt gac Ala G u Pro Thr lie Phe G u Val Pro G y Asp 250 255 tag <210> 284 <211> 256 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 284 IVet Ser Ser Thr Arg Ile Pro Val I e Ala Leu 1 5 10 Arg G y Thr Arg Leu G y G y Pro lie Pro Lys 25 Arg G u Arg Thr Leu Leu G u Arg Ser Leu G n 40 Su Ser Val Asp u Ile Ile Ile Leu Val Ser 55 Tyr Ala Arg Asp Leu Leu Arg Lys Arg Gy Leu 70 G y Val Arg Val Arg Leu Val His G y G y G y 90 Val Trp Ala y Leu G n Ala lie Ser Leu Asp 100 105 Ala lie Val Leu Ile His Asp Ser Ala Arg Ala 115 120 Page 364 at c SIle 145 ggc Sy caa G n aaa Lys agc Ser cca Pr o 225 cgc Ar g cca Pr o gga G y acc Thr ttc Phe ttg Leu 210 at g bet at c SIle gt a Val gt a Val cca Pr o ttc Phe 195 at g Net gcg Al a acc Thr ctg Leu gtt Val caa G n 180 gcc Al a gaa G u ttt Phe gac Asp taacccaatc atcccccgcg 888 891 Leu Al a Al a Pr o Leu G u Asp Leu Al a Phe bet Asp Asn Ar g Al a Thr 125 Al a Val Leu bet Asp Al a Thr 110 Pr o Al a Thr Thr G u Pr o Asp Pr o Pr o Met SIle 145 Gy G n Lys Ser Pr o 225 Ar g Al a Val Val Pr o Phe 195 bet Al a Thr Val Pr o Val 165 Gy Asp Tr p Lys G u 245 Ar g 135 Ser Thr Leu Asn Gy 215 Thr G u Gene Sequence 24-2-06.txt Lys Val His G u Gy Ala Thr 140 Asp Thr lie Lys Arg Val Ser 155 Pro Asn Arg Ala G u Leu Arg 170 Leu Ser G u Leu Val Ala Ala 185 190 Pro y Phe lie Pro Thr Asp 200 205 Ala Asp Val Val Cys Val G n 220 Thr Pro Ile Asp IVet IVet Leu 235 Pro Thr lie Phe G u Val Pro 250 Al a Pr o Al a 175 Asn Asp Gy Al a Gy 255 <210> 285 <211> 1056 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1033) <223> RXA00825 <400> 285 cccgttcatg ctgggctttg gtgcggtgat ggcaactatt tagtgcacgc cgat t ctgag aaacaactaa agtgagccac acc Thr aag Lys ct g Leu gaa G u aag Lys tct Ser ggt Sy ggc G y aat Asn gat Asp gag Su gt a Val ggc G y gag G u t cc Ser ct c Leu at t SIle cct Pr o at c SIle gtt Val gcg Al a gtt Val cac Hi s cac Hi s gga G y gt g Val gaa G u 45 gat Asp ct g Leu gac Asp t cc Ser at c I e gcc Al a ttc Phe gca Al a gcc Al a cat ct c Hi s Leu 15 gat aac Asp Asn acc gga Thr G y aac gag Asn u gcg caa Al a n 80 gaa acc G u Thr 95 Page 365 t gt ct gat ca t t gt gagtt t atg cgc aca gta gtt Net Arg Thr Val Val 1 gtt gac ctt ttg atc Val Asp Leu Leu Ile ctc tcc cgc gga cgc Leu Ser Arg Gy Arg aaa ctc acc ttt gtg Lys Leu Thr Phe Val ttt cta gga acc cac Phe Leu G y Thr His atc gat gtg cgc cac Ile Asp Val Arg His aac att ttg tcc acc Asn Ile Leu Ser Thr 100 Gene Sequence 24-2-06.txt atc cgc li e Ar g ttt acc Phe Thr gtt gat Val Asp 135 aag gt g Lys Val 150 tta gac Leu Asp gat cca Asp Pro ctg gga Leu G y gac t ac Asp Tyr 215 ggg gaa G y G u 230 acc tct Thr Ser aaa gat Lys Asp agt gca Ser Al a gag gt g G u Val 295 at c SIle t cc Ser 120 gaa G u t cc Ser tgt Cys cac Hi s ggc Gy 200 gt c Val at c SIle gac Asp gat Asp ct c Leu 280 aac Asn gac Asp ggc G y gt g Val gaa G u cac Hi s 170 gaa G u gac Asp gt c Val ggg Gy cag G n 250 gaa G u ttc Phe gaa G u gct Al a ggt G y cca Pr o at t SIle 155 at c SIle gca Al a acc Thr ggt Gy gga Gy 235 ct g Leu t at Tyr ggc G y caa G n gcc Al a t cc Ser gt g Val 140 t ac Tyr gca Al a gga G y aag Lys gac Asp 220 gag G u cac Hi s gca Al a aag Lys ggt G y 300 cgc cag Ar g at t SIle 125 gat Asp ct g Leu ccg Pr o gtt Val gt a Val 205 gt a Val cgc Ar g acc Thr cct Pr o gcc Al a 285 gt g Val G n 110 t ac Tyr cca Pr o aac Asn gca Al a gt g Val 190 ttc Phe gtt Val ttc Phe ct c Leu gca Al a 270 aaa Lys gcc Al a cac ggt gtt Hi s G y Val ggt gag cct G y u Pr o cat t cc cct His Ser Pro 145 acc ttc cgc Thr Phe Ar g 160 aat gtt tac Asn Val Tyr 175 gcc att ttc Al a i e Phe ggc gac ggc Sy Asp y cgt gct ttc Arg Ala Phe 225 aac att ggc Asn I e G y 240 gtg gcc act Val Al a Thr 255 cgt ct c ggc Arg Leu G y gag gtg ct t G u Val Leu aag act gt g Lvs Thr Val cgc aag gtt Arg Lys Val 115 tcg gaa ttc Ser G u Phe 130 tat gcg gca Tyr Al a Al a cac ct g t ac Hi s Leu Tyr ggc cca cgc G y Pro Arg 180 gcg ctg cga Ala Leu Arg 195 gga aac acc G y Asn Thr 210 tac ct g gct Tyr Leu Al a acc tct gt g Thr Ser Val gcg gca ggt Al a Al a G y 260 gat gtg cca Asp Val Pro 275 ggt t gg gag G y Trp G u 290 gag tac ttc G u Tyr Phe 451 499 547 595 643 691 739 787 835 883 931 979 1027 1056 act cac taggggaaaa tccaccacaa atc Thr His 310 <210> 286 <211> 311 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 286 Net Arg Thr Val Val Thr Gy y Ala G y Phe IIe Gy Ser His Leu 1 5 10 Val Asp Leu Leu I I e Lys G u G y His G u Val Val Val lie Asp Asn Page 366 Gene Sequence 24-2-06.txt Leu Ser Asp Ala Leu Si Lys L Phe L Ile A- Asn II Val Ai Pro Si 1 Pro T, 145 Arg Hi Tyr G Phe Al 2 Phe T, 225 G y TI Thr Al 3 y A Leu G 2! Val G 305 <210> <211> <212> <213> <220> <221> <222> <223> <400> Ar g Thr Gy Val Leu Lys 115 G u Al a Leu Pr o Leu 195 Asn Leu Ser Al a Val 275 Tr p Tyr Ar g Val Hi s Hi s Thr Val Pr o Ser Gy 165 G n Leu Ar g Ser G u 245 Ser Ar g Pr o Ar g G u Al a Pr o Val Ar g Thr Asp 135 Val Asp Pr o Gy Tyr 215 G u Ser Asp Al a Val 295 Hi s Leu Val Asp Al a 105 Ser Thr Gy Ser Gy 185 Leu Tyr Gy Ar g Pr o 265 Ser SIle Asp Phe 75 Leu Al a Gy Pr o SIle 155 I le Al a Thr Gy Gy 235 Leu Tyr Gy G n Val Hi s Hi s Al a Ser Val 140 Tyr Al a Gy Lys Asp 220 G u Hi s Al a Lys 0y 300 G u Asp Leu Asp Ar g SIle 125 Asp Leu Pr o Val Val 205 Val Ar g Thr Pr o Al a 285 Val Al a Thr G y Phe Al a Al a G n 110 Tyr Pr o Asn Al a Val 190 Phe Val Phe Leu Al a 270 Lys Al a Asn Al a G u Hi s Gy Hi s Thr Asn 175 Al a Gy Ar g Asn Val 255 Ar g G u Lys u G n Thr Gy G u Ser Phe 160 Val SIe Asp Al a SIe 240 Al a Leu Val Thr 287 1134 DNA Corynebacter i um gl utam cum CDS (1111) RXA01887 287 Page 367 Gene Sequence 24- 2- 06. txt catctttaca ggaaacccct tgacggcatc aatgggtggt atctagtatc tactagaacg ttatagtaga acgttctagt aaaacttgga aggatgaaaa t ca gt c aaa Ser Val Lys gcc ctc atc ggt Al a Leu I e G y gct Al a gga cgc atc gga Sy Arg lie y t ca Ser 15 aat cac gca cgc Asn His Ala Arg ct g at c Leu II e aca aac cac Thr Asn Hi s ccc aac gca Pro Asn Al a atc ggc tct gaa Ile G y Ser G u gtc gcc gtc gtt Val Al a Val Val gac cca act Asp Pro Thr gcg ttc tct Al a Phe Ser gaa acc ctc gct G u Thr Leu Ala gaa ttg ggc gcc G u Leu G y Al a aac cca Asn Pro gat gac gtc ctg Asp Asp Val Leu acc Thr cgc gat gac at t Arg Asp Asp Ile gcg gtt ttg att Ala Val Leu Ile gct Al a aca cca gca cga Thr Pro Ala Arg cac gcg gat ctc Hi s Al a Asp Leu gtg Val 80 gtc aaa gca gcg Val Lys Ala Al a gca Al a gcg ggc aag cac Al a G y Lys Hi s gt g Val ttt gtg gaa aag Phe Val G u Lys atg gcc gtc acc Net Al a Val Thr ct c gag Leu G u 100 gac gca gat Asp Al a Asp cag gtg ggc G n Val G y 120 gcc atc aac gca Ala lle Asn Al a cgc gaa gca aac Arg G u Ala Asn act gtc ctg Thr Val Leu 115 gca gca cgc Al a Al a Ar g ttc aat cgt cgt Phe Asn Arg Arg gcg gca ggt ttc Al a Al a y Phe gct Al a 130 gca cgc Al a Ar g 135 att gac gca ggc Ile Asp Al a G y gat Asp 140 atc ggc acc cca Ile y Thr Pro ctg ctt cgt tcc Leu Leu Arg Ser gtg Val 150 acc cgc gat cca Thr Arg Asp Pro gga 15y 155 cca ttc acc gct Pro Phe Thr Ala ccc aac aag atc Pro Asn Lys Ile cag tgg acc at c Sn Trp Thr Ile ttc Phe 170 ctg gaa acc ct c Leu G u Thr Leu cac gat ttc gat His Asp Phe Asp gcg ctg Al a Leu 180 tgc tac ctc Cys Tyr Leu cca ggc gca acc Pro G y Ala Thr gtt cca gaa gct Val Pro G u Al a 205 cca Pr o 190 gtg gaa gta acc Val G u Val Thr gct cac gct Al a Hi s Al a 195 ct c gac acc Leu Asp Thr gat tgc ctc gtc Asp Cys Leu Val 200 gct ggc act ggc Al a y Thr G y ttc Phe 210 gca gt g Al a Val 215 gtg act gtc Val Thr Val cgt ttt gat aac gga gca att ggt act gca gaa Arg Phe Asp Asn y Ala Ile y Thr Ala u 220 225 gcc tat ggt tat gac gtt cgc ggt gaa gtc ttc Al a Tyr y Tyr Asp Val Arg y G u Val Phe 235 240 245 gca Al a 230 agc ttc agc gca Ser Phe Ser Al a gga tcc aag ggc atg atg acc gca ggc Sy Ser Lys y Met Mbt Thr Ala y gac gcg cgc gca acc aac atg Asp Ala Arg Ala Thr Asn IVet Page 368 250 act ttc tac ggc gct gag ggc Thr Phe Tyr Gy Ala u y 265 acc gat ctg ctc tcc gat gct Thr Asp Leu Leu Ser Asp Al a 280 gac tcc atc cgt acc aac acc Asp Ser lie Arg Thr Asn Thr 295 300 cgc acc gca cta ctc atc gca Arg Thr Ala Leu Leu Ile Ala 310 315 ggc gca acc atc aac ctt gct Sy Ala Thr Ile Asn Leu Al a 330 ttttaaactc gca <210> 288 <211> 337 <212> PRT <213> Corynebacter i um gl ut ar Gene Sequence 24-2-06.txt 255 260 atc gcg gct gcc acc tca cgc gcg lie Ala Ala Ala Thr Ser Arg Ala 270 275 tac cga gct gaa ttc caa gct ttc Tyr Arg Ala G u Phe G n Ala Phe 285 290 cct tcc aag gtt cca ggc gaa gct Pro Ser Lys Val Pro G y G u Ala 305 ctc ggc gcc atc cga agc gta gaa Leu y Ala Ile Arg Ser Val u 320 gaa agc atc gag gtt taaccatgac Su Ser Ile u Val 335 931 979 1027 1075 1121 1134 <400> 288 Net Ser Val Lys 1 His Ala Arg Leu Val Val Asp Pro Ala Val Ala Phe Asp Al a Val Leu Val Lys Ala Al a Ala Val Thr Leu 100 Ala Asn Thr Val 115 Phe Al a Al a Al a 130 G n Leu Leu Arg 145 Pro Asn Lys Ile Asp Phe Asp Al a 180 Leu 5 I le Thr Ser SIle Al a G u Leu Ar g Ser Pr o 165 Leu Al a Thr Pr o Asn Al a 70 Al a Asp G n Al a Val 150 G n Cys Leu Asn Asn Pr o 55 Thr Sy Al a Val Ar g 135 Thr Tr p Tyr SIle Hi s Al a 40 Asp Pr o Lys Asp Sy 120 I le Ar g Thr Leu Sy Val 25 G u Asp Al a Hi s Ar g 105 Phe Asp Asp SIle Asn 185 Al a 10 SIle Thr Val Ar g Val 90 Al a Asn Al a Pr o Phe 170 Pr o Sy Sy Leu Leu Thr Phe l Ie Ar g Sy Sy 155 Leu Sy Ar g Ser Al a Thr Hi s Val Asn Ar g Asp 140 Pr o G u Al a SIle G u Asp Ar g Al a G u Al a Phe 125 I le Phe Thr Thr Sy Leu G u Asp Asp Lys Al a 110 Al a Sy Thr Leu Pr o 190 Ser Val Leu Asp Leu Pr o Ar g Al a Thr Al a SIe 175 Val Val Thr Ala His Ala Asp Cys Leu Val Val Pro G u Ala Ala y Thr Page 369 Gy I e 225 Ar g Ar g Thr Phe Pr o 305 Ar g Val 195 Leu Thr G u Thr Ar g 275 Al a G u Val Thr G u Phe 245 Mbt Asp Val Al a Thr 325 Al a Al a 230 Gy Thr Thr Asp Ar g 310 Gy Val 215 Ser Ser Phe Asp Ser 295 Thr Al a Gene Sequence 24-2-06.txt 200 205 Val Thr Val Arg Phe Asp Asn 220 Phe Ser Al a Al a Tyr G y Tyr 235 Lys Gy IVet IVet Thr Ala y 250 Tyr G y Al a G u G y Ile Al a 265 270 Leu Leu Ser Asp Ala Tyr Arg 280 285 lie Arg Thr Asn Thr Pro Ser 300 Al a Leu Leu Ile Al a Leu G y 315 Thr Ile Asn Leu Ala u Ser 330 ay Asp Asp 255 Al a Al a Lys Al a SIle 335 Al a Val 240 Al a Al a G u Val SIe 320 a u <210> 289 <211> 996 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(973) <223> RXN00013 <400> 289 ctgcagaaaa ttcgggacgc atgattgcac atattacccg acgccacctt attcagcaca cttggccgac ggcatgcaca aat Asn acc Thr cat Hi s at g Met gt g Val ctt gag G u aca Thr gcg Al a gaa G u acg Thr gcg aca Thr acc Thr gca Al a ggt Gy gcc Al a cgg ccc Pr o gt g Val gt g Val gat Asp cgt Ar g gag gct Al a at t SIle t ac Tyr 45 acg Thr gcg Al a gac gca Al a gct Al a aac Asn gat Asp gcc Al a ggc agc ct c Ser Leu 15 cac gat His Asp gct gga Al a G y tac aag Tyr Lys gag gcc G u Al a 80 gtg ctt Page 37C caccgattgt gattcttaga at g aa ggc atg act et G u Gy et Thr 1 gaa gac atg atc aaa Su Asp IVet lie Lys cag gat t ct gat gag G n Asp Ser Asp G u cgt ttg gca tgg cgc Arg Leu Ala Trp Arg act tct gtg tct gat Thr Ser Val Ser Asp ttc gtc gca ggc gtt Phe Val Ala y Val ggc gag gaa ggc gcg Leu O31u Al a Leu Ar g ene Sequence 24- 2- 06. txt Pr o 1u As p 1y ValI L e u (1y (1u 1u 1y Al a 95 100 gac cgg gcg Asp Ar g Al a ggc acc t ac G3 y Thr Tyr 120 gcg ct g gt c Al a Leu Val 135 gcc gt a cac Al a Val Hi s 1 50 at c cgc acc Ilie Ar g Thr ccc ct c aat Pro Leu Asn gcg gaa cct Ala O31u Pr o 200 gca acg ct g Al a Thr Leu 215 gcc gac ggc Al a Asp G3 y 230 gac t gg ct a Asp Trp Leu at c aaa gt g Ile Lys Val gaa gca gtt G3 u Al a Val 280 aaa Lys5 t tc Ph e ggc G31y cca Pr o ct c Le u 1 70 at c I Ie at t I Ie at g vb t at g G31y 250 gcc Al a gag (31u gga G31y c ag O31n cca Pr o 140 at g G31y ct g Le u aag Lys5 G31y 220 gca Al a gca Al a ggc G31y t cc Ser aaa Lys5 G31y 125 t cc Ser ggt G31y aag Lys5 gcc Al a gcg Al a 205 gcc Al a t gg Tr p ct c Le u gt c ValI g aa (31u 285 ac c Thr 110 t ca Ser gcg Al a t at Tyr gag (31u ac c Thr 1 90 t gg Tr p ggc G31y gt g ValI at c I Ie g aa (31u 270 t gg Tr p g at As p cca Pr o ac g Thr ct a Le u 1 75 t ac Tyr at g vb t t cc Ser c ag O31n g aa O31u 255 t gg Tr p gt c ValI t at Tyr t cg Ser t gg Tr p 1 g at As p at c I Ie ag c Ser at c I Ie c ac Hi s 240 ggc G31y t cc Ser g ac As p t gc cys 130 gt g ValI ggt G31y ct t Le u ccg Pr a gcc Al a 210 tt g Le u gt c ValI ggc G31y gca Al a ccg Pr a 115 t cg Ser ct t Le u ggc G31y gt c ValI t ca Ser 1 ac c Thr gcc Al a gca Al a gga G31y gga G31y 275 gt t ValI gcg Al a t tc Ph e ccg Pr a g ac As p 1 cgc Ar g c ac Hi s aac As n g at As p gca Al a 260 aac As n act t ta agc gca Thr Leu Ser Al a ct a gac Leu Asp 290 t aacaacaca t gagcaaat a t gc <210O> 290 <21 1> 291 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 290 'b t (31u G31y I'bt Thr Asn Pro Ou On Thr His Pro Ala Ala Ser Leu 1 5 10 Ou Asp I'bt Ilie Lys Thr I Ie Thr Lys Thr Phe Val IlieAla His Asp 25 (31n As p Se r As p (3u His Leu Ala O1n Al a Leu Val Tyr Asn Al a (31y 40 Page 371 Gene Sequence 24-2-06.txt Ar g Thr Phe Gy SIle Tr p Ar g 145 Phe Leu Hi s Val Asp 225 Ser Val Val Al a Al a Val Al a G u Pr o 115 Ser Leu Gy Val Ser 195 Thr Al a Al a Gy Gy 275 Asp Ar g Asp Val Al a Asp Leu Gy Gy 165 Al a SIle Pr o l Ie Tr p 245 Cys Al a bet Val 70 Leu Asp Gy Al a Al a 150 I le Pr o Al a Al a Al a 230 Asp SIle G u Ar g Val G u Ar g Thr Leu 135 Val Ar g Leu G u Thr 215 Asp Tr p Lys Al a G u Asn G y Val Thr Al a Al a Tyr 120 Val Hi s Thr Asn Pr o 200 Leu Gy Leu Val Val 280 Asp Leu Ser 105 Asn G u Ar g Thr G n 185 Asp Ar g Ser Pr o Thr 265 Ser Al a Ar g 90 Lys Phe Gy Pr o Leu 170 I e I e et et Gy 250 Al a G u Asp Pr o Ser Thr Asp Al a 155 Asp Ser G n Phe Gy 235 Ar g Gy lie Asp Ar g G u Gy G n Pr o 140 Net Gy Leu Lys Gy 220 Al a Al a Gy Ser Thr Asp Tyr Lys Al a Asp Lys Gy 125 Ser Gy Lys Al a Al a 205 Al a Tr p Leu Val G u 285 Al a Gy Thr 110 Ser Al a Tyr G u Thr 190 Tr p Gy Val SIle G u 270 Thr G u Val Tr p Asp Pr o Thr Leu 175 Tyr Net Ser G n G u 255 Tr p Leu <210> 291 <211> 996 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(973) <223> FRXA00013 <400> 291 ctgcagaaaa ttcgggacgc atgattgcac atattacccg caccgattgt gattcttaga acgccacctt attcagcaca cttggccgac ggcatgcaca atg gaa ggc atg act 115 Met G u G y lt Thr 1 aat cca gag cag aca cat ccc gct gca agc ctc gaa gac atg atc aaa 163 Page 372 Asn Pro G u n Thr His Pro Gene Sequence 24-2-06.txt Ala Ala Ser Leu G u Asp NIet IIe Lys 15 acc Thr cat Hi s at g Net gt g Val ctt Leu gac Asp ggc G y gcg Al a gcc Al a 150 at c SIle ccc Pr o gcg Al a gca Al a gcc Al a 230 gac Asp at c SIle aca Thr gcg Al a gaa Gu acg Thr gcg Al a gcg Al a t ac Tyr 120 gt c Val cac Hi s acc Thr aat Asn cct Pr o 200 ct g Leu ggc Gy ct a Leu gt g Val acc Thr gca Al a ggt Gy gcc Al a cgg Ar g aaa Lys ttc Phe ggc G y cca Pr o ct c Leu 170 at c SIle at t SIle at g Net at g let ggc Gy 250 gcc Al a tt c Phe ct g Leu gt g Val gat Asp 75 cct Pr o agc Ser acc Thr gat Asp gcc Al a 155 gac Asp t cc Ser caa G n tt c Phe ggc Gy 235 cgc Ar g ggc Gy gt g Val gt g Val gat Asp cgt Ar g gag G u gga Gy cag G n cca Pr o 140 at g let ggc G y ct g Leu aag Lys ggc Gy 220 gca Al a gca Al a ggc Gy at t SIle t ac Tyr 45 acg Thr gcg Al a gac Asp aaa Lys ggc G y 125 t cc Ser ggt G y aag Lys gcc Al a gcg Al a 205 gcc Al a tgg Tr p ct c Leu gt c Val gct Al a aac Asn gat Asp gcc Al a ggc Gy acc Thr 110 t ca Ser gcg Al a t at Tyr gag G u acc Thr 190 tgg Tr p ggc Gy gt g Val at c SIle gaa G0u 270 cac Hi s gct Al a t ac Tyr gag G u gt g Val 95 tgg Tr p gat Asp cca Pr o acg Thr ct a Leu 175 t ac Tyr at g let t cc Ser cag G n gaa G u 255 tgg Tr p gat Asp gga Gy aag Lys gcc Al a 80 ctt Leu gt c Val t at Tyr tcg Ser tgg Tr p 160 gat Asp at c SIle agc Ser at c SIle cac Hi s 240 ggc Gy t cc Ser gat Asp ttg Leu tct Ser gt c Val gag G u gac Asp tgc Cys 130 gt g Val ggt G y ctt Leu ccg Pr o gcc Al a 210 ttg Leu gt c Val ggc Gy gca Al a tct Ser gca Al a gt g Val gca Al a gaa G u ccg Pr o 115 tcg Ser ctt Leu ggc G y gt c Val t ca Ser 195 acc Thr gcc Al a gca Al a gga Gy gga G5y 275 gat Asp tgg Tr p tct Ser ggc Gy ggc Gy 100 gtt Val gcg Al a ttc Phe ccg Pr o gac Asp 180 cgc Ar g cac Hi s aac Asn gat Asp gcg Al a 260 aac Asn gag G u cgc Ar g gat Asp gtt Val gcg Al a gat Asp ct c Leu ggc G y gga G y 165 gcc Al a at c SIe cct Pr o at c SIe tgg Tr p 245 tgc Cys gcg Al a gaa gca gtt agt gag atc tcc gaa act tta agc gca cta gac Page 373 ene Sequence 24- 2- 06. txt (31u Al a Val Ser O31u I Ie Ser (31u Thr Leu Ser Al a Leu Asp 280 285 290 t agcaacaca t gagcaaat a t gc <210> 292 <211> 291 <21 2> PRTF <21 3> Cor ynebact er i urn gi ut ani cumn <400> 292 [vb t O31u (1y O31u O31n Ar g Th r Ph e G31y I Ie Tr p Ar g 145 Ph e Le u Hi s ValI As p 225 Ser ValI ValI Al a vb t Ser Al a ValI Al a O31u Pr o 115 Ser Le u G31y ValI Ser 1 95 Th r Al a Al a G31y G31y 275 As p I'bt Thr Asn Pro Ilie Lys Thr Ilie As p 03 u Hi s Leu Tr p Ar g I'b t Ar g Ser As p Val Val 70 G31y Val Leu O31u G31y Al a As p Ar g 1 00 Val As p G31y Thr Al a Leu Al a Leu 135 Phe 03 y Al a Val 150 Pro 31y Ilie Ar g 1 65 Asp Al a Pr o Leu 1 80 Ar g Ilie Al a O31u Hi s Pr o Al a Thr 215 Asn Il e Al a Asp 230 Asp Trp Asp Trp 245 Al a Cys IlIe Lys 260 Asn Al a 03 u Al a O31u Thr Al a 40 O31u Thr Al a Al a Tyr 120 ValI Hi s Thr As n Pr o 200 Le u G31y Le u ValI ValI 280 O31n Lys5 25 O31n As n As p Le u Ser 1 05 As n (31u Ar g Thr O31n 1 85 As p Ar g Ser Pr o Thr 265 Ser Thr 10 Thr Al a O31y Al a Ar g 90 Lys5 Ph e O31y Pr o Le u 1 70 le le vb t vb t O31y 250 Al a (31u Hi s Ph e Le u ValI As p 75 Pr o Ser Thr As p Al a 1 55 As p Ser O31n Ph e O31y 235 Ar g O31y I Ie Pr o ValI ValI As p Ar g O31u O31y O31n Pr o 140 vb t O31y Le u Lys5 O31y 220 Al a Al a O31y Ser Al a I Ie Tyr Thr Al a As p Lys5 O31y 125 Ser O31y Lys5 Al a Al a 205 Al a Tr p Le u ValI O31u 285 Al a Al a As n As p Al a O31y Thr 110 Ser Al a Tyr O31u Thr 1 Tr p O31y ValI I Ie O31u 270 Thr Ser Hi s Al a Tyr O31u ValI Tr p As p Pr o Thr Le u 1 Tyr vb t Ser O31n O31u 255 Tr p Le u Le u As p O31y Ly s Al a Le u ValI Tyr Ser Tr p 1 As p I Ie Ser I Ie Hi s 240 O31y Se r Se r Page 374 ene Sequence 24- 2- 06. txt <210O> 293 <21 1> 948 <212> DNA <213> COr ynebact er i urn gi ut arr cumn <220> <221 CDS <222> (925) <223> RXA01099 <400> 293 ggat gagggc at tgat tccg t cat cat tgg caaggcact t t at gagcaca agt tcaccct cgaagaggct t tggct gcag t agaaaagct cggt t aat ac at g gat gct cgt l'bt Asp Ala Arg at g vb t at g vb t tt t Ph e ct g Le u gcg A a aac As n gt c ValI aaa Ly s cct Pr o tt t Ph e 1 50 cgt Ar g at t I Ie gcc A a ggc G31y ac g Th r at g vb t t cc Ser gcg A a g ac As p ct c Le u 120 g aa (31u t cc Ser g at As p ggt G31y gcg A a gga G31y gt g ValI ac a Th r ac g Th r t cc Ser ccc Pr o gct A a tt g Le u gcc A a ct g Le u 1 70 gt g ValI gag (31u gct A a g at As p ggc G31y cgc Ar g 75 aac As n gt c ValI tt t Ph e caa O31n t cc Ser 155 acc Th r ggt (31y gcc A a gca A a at g vb t at t lIe t gg Tr p ccg Pr o ct g Le u g aa (31u g aa (31u 140 ccg Pr a gag (31u gt t ValI gt t ValI cct Pr a gcc A a 45 gct A a gt g ValI at g vb t ggt G31y ggt G31y 125 c aa O31n cgc Ar g ct c Le u g at As p gt a ValI gcc A a at c I Ie gt c ValI at t I Ie ag c Ser at t I Ie 110 t ca Ser t cc Ser aat As n ac g Th r ct c Le u 1 90 g at As p 15 cat Hi s g aa O31u at c I Ie g at As p gcg A a 95 ac c Th r ccg Pr a agt Ser ac a Th r g aa O31u 1 75 gcg A a g at As p at g vb t t cc Ser ggt G31y ccc Pr a 80 at c I Ie t cc Ser ct g Le u tt g Le u gcg A a 1 60 t cg Ser tt c Ph e 1 gcc A a aaa Ly s cat Hi s g aa O31u at c I Ie ct g Le u at g vb t at g vb t gt a ValI 145 tt t Ph e t at Tyr act Th r g aa O31u t cc Ser at g vb t g aa O31u g ac As p gt g ValI ccc Pr a at c I Ie 130 t cc Ser cct Pr a ct t Le u gcg A a gcc A a ccg Pr a cgt Ar g ggt G31y ggc G31y t ct Ser at g vb t 115 aac As n c ac Hi s gt g ValI cgt Ar g c ag O31n 1 ct c Le u ggg G31y t cg Ser ggc G31y ac c Th r tt g Le u 1 00 ct g Le u ggt G31y at t I Ie gag (31u ccc Pr a 1 ggc G31y ggg G31y tt c Ph e g at As p at g vb t ggt G31y gcc A a ct t Le u ggt G31y g aa (31u ggt G31y tt g Le u 1 cgc Ar g at t I Ie ttt gga gca tgc gta tcg ttt agt cct cat gtt t gg gac aat t cc gca Page 375 Phe G31y Ala Cys Val Ser Phe 200 ggc gt g at g t tg at g cgc gct G31y Val [Vb t Leu [Vb t Ar g Al a 215 220 ggc cat ccg tgg gca cca ggt G31y Hi s Pr o Tr p Al a Pr o G31y 230 235 gct cac gat gt g ct g t t a agt Al a Hi s Asp Val Leu Leu Ser 250 gca gat gca ggt aat gac cag Al a Asp Al a G3 y Asn Asp G3 n 265 t aaaat gggc gt ggcaat tc gag <210> 294 <211> 275 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ai ene Sequence 24- 2- 06. txt Ser Pro Hi s Val Tr p Asp Asn 205 210 gct ggt gca caa gt t act gac Ala (31y Al a O31n Val Thr Asp 225 agg gga gt c gt g gcc gga aca Ar g G31y Val Val Al a G31y Thr 240 aag at t gaa aaa gt t cgg tt g Lys Ilie O31u Lys Val Ar g Leu 255 t cg t ta aat gag gag t ac aag Ser Leu Asn 03 u 03 u Tyr Lys 270 275 Ser Al a acc gaa Thr 03 u aaa agg Lys Ar g 245 at g cat I'b t Hi s 260 <400> 294 [vb t As p Al a Al a Hi s O31u li1e Le u vb t vb t ValI 145 Ph e Tyr Thr Al a Pr o Ar g G31y G31y Ser vb t 115 As n Hi s ValI Ar g O31n 1 95 Ar g G31y [Vbt Leu Phe I'bt (3 y Asp Phe Ser I'b t Leu O3 y 03 y Al a 70 Thr Al a As n Leu Leu Val 1 00 Leu 03 y Lys G31y O31u Pr o Il e 03 y Phe 150 (31u Leu Ar g 1 65 Pr o Ar g Ilie 1 80 03 y Il e Phe O31n Al a As n Thr Ph e As p Ar g O31n 135 Ser Ar g Thr O31y O31y Thr 40 vb t Ser Al a As p Le u 120 O31u Ser As p O31y Al a 200 Leu Al a I I e Ph e 25 O31u vb t O31y Al a O31n 1 05 Thr Pr o vb t Le u Ser 1 85 Cys Al a 10 O31y ValI Thr Thr Ser 90 Pr o Al a Le u Al a Le u 1 70 ValI ValI O31u Al a As p O31y Ar g As n ValI Ph e O31n Ser 1 Thr O31y Ser Al a Al a vb t I Ie Tr p Pr o Le u O31u O31u 140 Pr o O31u ValI Ph e ValI Pr o Al a Al a ValI vb t O31y O31y 125 O31n Ar g Le u As p Ser 205 ValI Al a li1e ValI I Ie Ser I Ie 110 Ser Ser As n Thr Le u 1 Pr o As p Hi s Ou le As p Al a Thr Pr o Ser Thr O31u 1 Al a Hi s Trp Asp Asn Ser Al a 0 y Val I'bt Leu I'bt Arg Ala Ala Oy Ala On Page 376 Gene Sequence 24-2-06.txt 210 215 220 Val Thr Asp Thr G u G y His Pro Trp Ala Pro Gy Arg Gy Val Val 225 230 235 240 Al a G y Thr Lys Arg Al a His Asp Val Leu Leu Ser Lys Ile G u Lys 245 250 255 Val Arg Leu Iet Hi s Al a Asp Al a G y Asn Asp G n Ser Leu Asn G u 260 265 270 G u Tyr Lys 275 <210> 295 <211> 576 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> <223> RXN01332 <400> 295 cac at c t ct His Ile Ser 1 gca gaa gac Al a G u Asp at c gca gcc I e Al a Al a ttc cat gag Phe Hi s G u ctg tgt gaa Leu Cys G u gtc gag ct g Val G u Leu atg cgc cgc IVet Arg Arg tcc ggc gaa Ser G y G u 115 cca agt gt t Pro Ser Val 130 gtc gtc cac Val Val Hi s 145 gtc tcc gtt Val Ser Val att I e 5 ccg Pr o gct Al a gt a Val cca Pr o cag G n gac Asp ggc Gy gag G u ttc Phe gt g Val att gag ccc gac gca gca cgt gcc gct Ile u Pro Asp Ala Ala Arg Ala Ala 10 gca Al a gac Asp gt c Val 55 acc Thr ct g Leu gag G u ct g Leu t ac Tyr 135 gt c Val t ac Tyr cag G n gca Al a 40 cca Pr o cca Pr o gac Asp t ac Tyr ct c Leu 120 acc Thr at c SIle cca Pr o gcc ttc Al a Phe gtg ct g Val Leu gca ct a Al a Leu gat tct Asp Ser aag cca Lys Pro 90 aac aac Asn Asn 105 at g ct c IVet Leu cag t cc G n Ser cca tgg Pro Tr p aag acc Lys Thr act Thr at c SIle gaa G u gaa G u 75 cac Hi s ttg Leu cgc Ar g at g bet ct c Leu 155 cgc Ar g gcc Al a gca Al a t cc Ser at c SIle cgc Ar g ggc Gy ct g Leu 140 gca Al a att SIle gt a Val ggc Gy t ca Ser cag G n aaa Lys ct g Leu 125 at c SIle ggc Gy gaa G u cca Pr o ctt Leu ct g Leu gtt Val ttg Leu 110 cac Hi s acc Thr t cc Ser gca gct Al a Al a gat gct Asp Al a ggt cag G y G n ccc atc Pro Ile cgc atc Arg Ile ggt ttc G y Phe gtg gaa Val G u cgc aac Arg Asn gac tcc Asp Ser cga gtt Arg Val r\ t cc tca ctg gcg cac tcc Ser Ser Leu Ala His Ser Page 377 Gene Sequence 24-2-06.txt 165 170 175 ggc ctc aag gaa cca atc ctg gtg atc atg gag ctc gaa aac ggc gtg Gy Leu Lys u Pro Ile Leu Val lie Vet G u Leu G u Asn y Val 180 185 190 <210> <211> <212> <213> 296 192 PRT Cor ynebact eri um gl ut ari cum <400> 296 His Ile Ser 1 Al a u Asp I e Al a Al a Phe Hi s G u Leu Cys G u Val G u Leu Iet Arg Arg Ser G y G u 115 Pro Ser Val 130 Val Val Hi s 145 Val Ser Val G y Leu Lys Ala I e 5 Ala Pro Asp Al a Pro Val Lys Pro G u G n Phe Asp 100 Al a G y G y G u G u Phe G u Val 165 G u Pro 180 lie u Pro Asp Al a Asp Val 55 Thr Leu G u Leu Tyr 135 Val Tyr Leu G n Al a 40 Pr o Pr o Asp Tyr Leu 120 Thr SIle Pr o Val Al a 25 Val Al a Asp Lys Asn 105 bet G n Pr o Lys SIle 185 Al a 10 Phe Leu Leu Ser Pr o 90 Asn Leu Ser Tr p Thr 170 vet Al a Thr SIle G u G u 75 Hi s Leu Ar g et Leu 155 Ser a u Ar g Ar g Al a Al a Ser SIle Ar g Sy Leu 140 Al a Ser Leu Al a SIle Val Sy Ser G n Lys Leu 125 I le Sy Leu a u Al a G u Pr o Leu Leu Val Leu 110 Hi s Thr Ser Al a Asn 190 Al a Asp Sy Pr o Ar g Sy Val Ar g Asp Ar g Hi s 175 Sy Al a Al a G n SIe I e Phe G u Asn Ser Val 160 Ser Val <210> 297 <211> 549 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> FRXA01332 <400> 297 gca gca cgt gcc gct gca gct gca gaa gac gcg ccg ggt gca cag gcc Ala Ala Arg Ala Ala Ala Ala Ala u Asp Ala Pro y Ala n Ala 1 5 10 Page 378 Gene Sequence 24-2-06.txt ttc Phe ct g Leu ct a Leu tct Ser cca Pr o aac Asn ct c Leu t cc Ser tgg Tr p 145 acc Thr at g bet cgc Ar g gcc Al a gca Al a t cc Ser at c SIle cgc Ar g ggc Gy 115 ct g Leu gca Al a t ca Ser ct c Leu att gaa gat gct Ile u Asp Ala atc gca gcc gat gct gtc lie Ala Ala Asp Ala Val cca Pr o ctt Leu ct g Leu gtt Val ttg Leu cac Hi s acc Thr t cc Ser gcg Al a 165 aac Asn cag G n at c I e at c SIle ttc Phe gaa G u aac Asn t cc Ser 135 gtt Val t cc Ser gtg Val ttc Phe 40 ct g Leu gt c Val at g Net t cc Ser cca Pr o 120 gt c Val gt c Val ggc Gy cat Hi s tgt Cys gag G u cgc Ar g ggc Gy 105 agt Ser gt c Val t cc Ser ct c Leu gag G u gaa G u ct g Leu cgc Ar g 90 gaa G u gtt Val cac Hi s gtt Val aag Lys 170 cca Pr o aag Lys gag G u 75 ttc Phe gct Al a ggt Gy gaa G u gaa G u 155 gaa G u gt a Val cca Pr o cag G n gac Asp ggc Gy gag Gu ttc Phe 140 gt g Val cca Pr o ctt Leu ct g Leu aag Lys cct Pr o gaa G u agc Ser 125 gat Asp aag Lys at c SIle gac gca gtg Asp Al a Val gt c cca gca Val Pro Al a acc cca gat Thr Pro Asp ctg gac aag Leu Asp Lys gag tac aac G u Tyr Asn ct g ct c at g Leu Leu Ibet 110 tac acc cag Tyr Thr G n gt c at c cca Val Ile Pro tac cca aag Tyr Pro Lys 160 ct g gt g at c Leu Val Ile 175 <210> 298 <211> 183 <212> PRT <213> Corynebact <400> 298 Ala Ala Arg Ala 1 Phe Thr Arg lie Leu Ile Ala Val Leu G u Al a G y Ser G u Ser Ser Pro His Ile n er i um gl ut am cum Ala Al a Ala Ala G u 5 G u Asp Ala lie Ala 25 Pro G y G n Phe His Leu Pro lie Leu Cys 55 Leu Arg Ile Val G u 70 Val G y Phe Iet Arg Asp Al a Pro 10 Al a Asp Al a Su Pro Val G u Lys Pro Leu G u G n 75 Arg Phe Asp 90 Page 379 Al a Asp Val Thr Leu G u G n Al a Pr o Pr o Asp Tyr Al a Val Al a Asp Lys Asn Asn Leu Ser Tr p 145 Thr Met Ar g Gy 115 Leu Al a Ser Leu Leu Hi s Thr Ser Al a 165 Asn G u Asn Ser 135 Val Ser Val Gene Sequence 24-2-06.txt Ser G y G u Al a y G u Leu Leu Met 105 110 Pro Ser Val y u Ser Tyr Thr G n 120 125 Val Val His G u Phe Asp Val Ile Pro 140 Val Ser Val G u Val Lys Tyr Pro Lys 155 160 Sy Leu Lys G u Pro lie Leu Val lie 170 175 <210> 299 <211> 1128 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1105) <223> RXA01632 <400> 299 aagggctgca acgtgctttc gacaccacca tcgcagcgtt ccccctccac taactgatct ttgaaaggct gaaaaaact c gcc Al a gct Al a gaa G u t ca Ser ggt Sy cgt Ar g at g et at g M\et ttc Phe aac Asn gca Al a gat Asp cca Pr o at t SIle cgt Ar g ggg G y 120 gct Al a gat Asp cgt Ar g gt g Val agc Ser gca Al a tgc Cys aac Asn ggc G y ctt Leu tt g Leu tt c Phe acc Thr 75 ct g Leu aaa Lys cga Ar g cgc Ar g gaa G u gca Al a gcc Al a cac Hi s tgc Cys gag G u cgc Ar g at c SIle ct c Leu gaa G u 45 cgc Ar g gtt Val gaa G u aag Lys ttc Phe 125 ggt G y gtt Val gcc Al a gat Asp gat Asp aaa Lys at c SIle 110 gat Asp gt c Val at c SIle ggg G y at c SIle at c I e 80 at t SIle gac Asp tct Ser tgaacaagct gctcgtctcg atg act ctt cgt atc 11! IVet Thr Leu Arg Ile 1 cac gct gcc aac att 16 His Ala Ala Asn l e gcc gat cct ttc att 21 Ala Asp Pro Phe Ile gca gaa gcg gtt gca Ala G u Ala Val Ala gat ggc atc gtg atc Asp y Ile Val lle acc cgc gcc gtg gaa Thr Arg Ala Val G u gat tta gac att gaa Asp Leu Asp Ile G u 100 ggc gct tcc aag gtg Sy Ala Ser Lys Val 115 ttc gct gcc atc aat 49! Phe Ala Ala lie Asn 130 Page 380 Gene Sequence 24-2-06.txt gcg Al a at c SIle 150 ggt Sy ttc Phe ttc Phe acg Thr cac Hi s 230 ggc Gy aat Asn gag G u gga Gy at c SIle 310 gt g Val cgc Ar g at c SIle gt g Val cag G n 200 cgt Ar g t cc Ser ct c Leu gaa G u t ac Tyr 280 cgc Ar g ctt Leu aac Asn cca Pr o cgc Ar g 170 aat Asn at c SIle t ca Ser ggc Gy gcc Al a 250 acc Thr gcc Al a ggc Gy ct a Leu cag G n gcg Al a 155 gat Asp at c SIle gcg Al a aag Lys tac Ty r 235 gac Asp gag G u gct Al a caa G n gcg Al a 315 gag atc gg Gu lie G 140 ccc gca cc Pr o Al a Pr at g acc at Met Thr II gt g gaa gt Val u Va 19 gag tt c aa G u Phe As 205 ggc gag tt G y G u Le 220 gac cag cc Asp G n Ar aac atc ac Asn Ile Ar cag gca ga G n Al a As 27 tac aag gc Tyr Lys Al 285 ggc ttc tc G y Phe Se 300 aat gca tg Asn Al a Cy y aac ctg gag Asn Leu G u 145 aag gac tac Lys Asp Tyr 160 cac gat ct g His Asp Leu 175 act gca acc Thr Al a Thr gac tac gac Asp Tyr Asp at c aac at c Ile Asn Ile 225 ctt gag gct Leu G u Al a 240 ccc acc acg Pro Thr Thr 255 ccg att ttc Pro Ile Phe gag ctc gca G u Leu Al a cca aac ttc Pro Asn Phe 305 ct t aaa t ca cag ttg gtg atc Sn Leu Val lle at c SIle gat Asp ggc G y cag G n 210 gt g Val ttc Phe gt g Val aac Asn act Thr 290 gag G u gct Al a gca ggt t cc Al a G y Ser 165 atg gcg cgt IVet Al a Arg 180 gcc aat gtt Al a Asn Val 195 gtt atc gtc Val Ile Val aac tcc cgc Asn Ser Arg ggc t ct aag G y Ser Lys 245 cgc aag cac Arg Lys His 260 ttc ttc ctc Phe Phe Leu 275 ttt gct caa Phe Al a G n gac ggc gtc Asp G y Val caa acc ggc G n Thr G y 325 547 595 643 691 739 787 835 883 931 979 1027 1075 1125 1128 Leu G u 320 Ser cgc acc gtc acc ctc aac cct gcc aac gtt tagtcaacgt ctagttaatg Arg Thr Val Thr Leu Asn Pro Ala Asn Val 330 335 cct <210> 300 <211> 335 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 300 IVet Thr Leu Arg IIe Ala Leu Phe Gy Ala G y Arg IIe Gy His Val 1 5 10 His Ala Ala Asn lie Ala Ala Asn Pro Asp Leu G u Leu Val Val lie 25 Page 381 Ala Asp Pro Phe lie Ou Gy Gene Sequence 24- 2- 06. txt Ala n Arg Leu Ala u Ala Asn y 40 Al a Asp Thr Asp Gy Phe G u 145 Tyr Leu Thr Asp SIle 225 Al a Thr Phe Al a Phe 305 Ser Al a SIle Al a Asp Ser 115 Al a Leu Al a bet Al a 195 Val Asn Gy Ar g Phe 275 Phe Asp G n Al a SIle G u G u Val Asn SIle Ser 165 Ar g Val Val Ar g Lys 245 Hi s Leu G n Val Gy 325 Pr o Ser Gy Val Leu Ar g 135 Ser Gy Phe Ser Leu 215 Cys bet Al a Ar g SIle 295 Al a Thr G u Thr Pr o Al a 105 Phe Al a Asp Phe Pr o 185 G u Gy Tyr Al a Ser 265 Asp Asp G u Thr Val Ser Al a 90 Cys Asn Asn Pr o Ar g 170 Asn SIle Ser Gy Al a 250 Thr Al a Gy Leu Leu 330 Phe Thr Leu Lys Ar g G n Al a 155 Asp SIle Al a Lys Tyr 235 Asp G u Al a G n Al a 315 Asn Al a Hi s Cys G u Ar g G u 140 Pr o bet Val G u Gy 220 Asp Asn G n Tyr Sy 300 Asn Pr o Ar g Val G u Lys Phe 125 I le Al a Thr G u Phe 205 G u G n SIle Al a Lys 285 Phe Al a Al a Asp Leu Pr o Sy Pr o Asn Lys Hi s 175 Thr Asp SIe Leu Pr o 255 Pr o G u Pr o Leu Val 335 <210> 301 <211> 1206 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1183) <223> RXA01633 Page 382 Gene Sequence 24-2-06.txt <400> 301 gcgaatgcat gccttgaatc agctcaaacc ggccgcaccg tcaccctcaa ccctgccaac gtttagtcaa cgtctagtta atgcctaagg agaaaacct c atg aaa aac atc acc IVet Lys Asn Ile Thr at c SIle at g let at c SIle gac Asp ggg Gy gtt Val ccg Pr o gat Asp ct c Leu tgg Tr p 150 gt g Val cac Hi s gt g Val ggt Gy ct c Leu 230 at g Net gcc Al a ct c Leu ggc Gy gat Asp cgc Ar g gcg Al a gct Al a 120 t ac Tyr cat Hi s t ac Tyr ttc Phe gcc Al a 200 at c SIle acc Thr ggc Gy gct Al a gac Asp aag Lys ctt Leu ggc G y gat Asp gcg Al a gaa G u ct c Leu gct Al a 170 at c SIle ggc Gy acc Thr at c SIle gt c Val gaa G u gca Al a gcg Al a tt c Phe 75 at c SIle gt g Val acg Thr gct Al a aag Lys 155 acc Thr at t SIle agc Ser gga Gy gcc Al a 235 ggc Gy act Thr at g Net gcg Al a at t SIle gcg Al a cct Pr o gtt Val aaa Lys 140 gcc Al a tct Ser cgc Ar g aac Asn gcg Al a 220 act Thr cgc Ar g ttg Leu ccc Pr o 45 gca Al a gcc Al a gca Al a gag G u cag G n 125 cga Ar g gt a Val ggt Gy tgg Tr p aac Asn 205 gcc Al a cgt Ar g at t SIle aag Lys ggt Gy agc Ser acc Thr aag Lys tcg Ser 110 gt c Val cgc Ar g tcg Ser gga Gy ct g Leu 190 ggc Gy ct a Leu t ac Tyr ggc Gy 15 gaa Gu ttt Phe gt c Val agc Ser ct g Leu 95 ct g Leu ggc G y ct a Leu agc Ser ctt Leu 175 acc Thr gac Asp ct c Leu aac Asn cgc Ar g cgc Ar g gcg Al a gat Asp acc Thr ccg Pr o aac Asn cac Hi s gat Asp gat Asp 160 ttc Phe ggc Gy cca Pr o acg Thr ggt G0y 240 1 at g let gac Asp gag Gu aag Lys gct Al a at g let at c SIle cag G n gca Al a 145 gcg Al a cgc Ar g cag G n gaa G u ctt Leu 225 gca Al a cac Hi s ct c Leu cag G n ctt Leu ggc Gy ttc Phe at c SIle cgc Ar g 130 ggc G y ttt Phe gat Asp gat Asp at c SIle 210 gcc Al a ggc Gy gcc Al a att SIe ggc Gy gag G u gtc Val gag G u 100 gaa G u ttt Phe ct c Leu cca Pr o tcg Ser 180 gtc Val gca Al a ggc Gy gac Asp cgc ctc gat gtt atg ggc tct aaa gat tcc acg atc gtt ggc ctg gat Page 383 Ar g gaa G u ggc Gy aat Asn cct Pr o 310 gct Al a cgg Ar g gtt Val Asp tct Ser tcg Ser 280 tgc Cys acc Thr ct g Leu at t SIle tct Ser 360 Gene Sequence 24-2-06.txt Met G y Ser Lys Asp Ser Thr lie Val y 250 255 ttc gct tct gcg gag gag ggc atc gat ttc Phe Ala Ser Al a G u G u G y Ile Asp Phe 270 275 cca acg ttt gcc gag cgc ttc gcc gac gca Pro Thr Phe Ala G u Arg Phe Ala Asp Ala 285 290 gcg ttc gtg gag ttg atc ctg gga gag cgg Ala Phe Val G u Leu Ile Leu Gy G u Arg 300 305 gca gac gct gtg gct gcg gcg att gtt gcc Ala Asp Ala Val Ala Ala Ala lle Val Ala 315 320 ctg gtc act ggc gag cca gtg aag att cct Leu Val Thr Gy G u Pro Val Lys lie Pro 330 335 gaa ggt tct gcg cag cca gtt gag gtg cgt Su y Ser Ala n Pro Val u Val Arg 350 355 taaaacct t a ctgcttatct aaa Leu 260 cca Pr o t ac Tyr gaa G u gat Asp act Thr 340 gcg Al a Asp acc Thr aag Lys aac Asn gca Al a 325 gt a Val ct g Leu 931 979 1027 1075 1123 1171 1206 <210> 302 <211> 361 <212> PRT <213> Corynebacter i um gl utam cum <400> 302 IVet Lys Asn Ile Thr Ile Gy IVet Val 1 5 Net His Val Ala Asn Net Leu Ala Val Asp Leu Asn lie G u ie Val Leu Ala 40 G u n Val y Ala Asp et y Val 55 Lys Leu Ile G u Asp G y Val Asp Al a 70 Ala G y His Val Asp Val Leu Arg Lys Iet Phe Cys G u Lys Pro Ile Ala Ser 100 105 lie lie Arg G u lie Asp Al a Al a G y 115 120 G n Arg Arg Phe Asp Leu y Tyr G n 130 135 Ala G y Asp Leu G y Trp Leu His Ser 145 150 Sy Val y 10 Al a G u Thr Asp Al a IVet Lys Al a Al a Leu Phe Il e G y I I e Al a 90 Asp Val Pr o Al a Thr Val G u Al a Lys 140 Leu Lys Al a 155 Page 384 Ar g Leu Pr o Al a Al a Al a G u G n 125 Ar g Val Sy G u Phe Val Ser Leu Leu Sy Leu Ser Ala Phe Pro Pro Pro Val Ser 165 Gene Sequence 24-2-06.txt Tyr Cys Al a Thr Ser G y G y Leu Phe Ar g G n G u Leu 225 Al a SIle SIle Al a Gy 305 I le Lys G u Val SIle 195 Gy Asp Hi s Gy Phe 275 Al a Ar g Al a Pr o Ar g 355 Leu G u Val Thr Val 245 Asp Thr Lys Asn Al a 325 Val Leu Hi s Val Gy Leu 230 Ar g G u Gy Asn Pr o 310 Al a Ar g Val Asp Tyr Asp 215 Al a Leu Lys G u G u 295 Cys G n G u Pr o Phe Al a 200 I le Thr Asp Ser Ser 280 Cys Thr Leu SIle Ser 360 Asp 185 Lys Asp Al a Val Al a 265 Hi s SIle Pr o Ser Leu 345 Al a 170 I le Gy Thr l Ie Met 250 Phe Pr o Al a Al a Leu 330 G u 175 SIle Ser Gy Al a 235 Gy Al a Thr Phe Asp 315 Val Gy Ar g Asn Al a 220 Thr Ser Ser Phe Val 300 Al a Thr Ser Tr p Asn 205 Al a Ar g Lys Al a Al a 285 G u Val Gy Al a Leu 190 Gy Leu Tyr Asp G u 270 G u Leu Al a G u G n 350 Thr Asp Leu Asn Ser 255 G u Ar g SIe Al a Pr o 335 Pr o <210> 303 <211> 1146 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1123) <223> RXN01406 <400> 303 gttcctcatt cctctaatcg gcgcactatc tttgcctcgc ttcctcctct t agaaaccca cttctgaaag gt at aaaaac gga ctc gt t G y Leu Val at c caa gct Ile G n Al a gaa ggc acc G u G y Thr ggc tac ggt gtc ggc ggc Sy Tyr Gy Val Gy y tct acg cac tgc gaa tta Ser Thr His Cys G u Leu 30 aaa gca gcc gtt gca gaa Lys Ala Ala Val Ala G u 45 agg ctc Arg Leu 15 gta ggc Val G y gat ctt Asp Leu Page 385 gacggcggtg cccgagcctt atg act att cga atc IVet Thr Ile Arg Ile 1 ttt cac acc cct tac Phe His Thr Pro Tyr gta gtt gct cgt tcc Val Val Ala Arg Ser cca gat gtt gcc atc Pro Asp Val Ala lle Gene Sequence 24-2-06.txt gt g Val at c SIle aac Asn gca Al a ct c Leu ct g Leu ct a Leu 150 ttg Leu at g bet cca Pr o tcg Ser t cc Ser 230 t ac Tyr aat Asn ttg Leu t ac Tyr tcg Ser acc Thr ggt Sy gcc Al a gt c Val 120 at c SIle ct g Leu cgc Ar g ccg Pr o ggc Gy 200 gcc Al a gaa G u gac Asp cgc Ar g acc Thr 280 cgc Ar g aca Thr cca Pr o gca Al a gaa G u cac Hi s gaa G u gaa G u ct g Leu 170 acc Thr acc Thr tcg Ser cgc Ar g cag G n 250 cac Hi s gaa G u t ac Tyr gaa G u gcc Al a 75 gt g Val ctt Leu aac Asn gaa G u cct Pr o 155 ggc Gy tct Ser aac Asn cac Hi s tt g Leu 235 acc Thr tgg Tr p ggc G y gat Asp ctc ctc gaa ctg ggc gt c Leu Leu G u Leu G y Val acg cgc cgg gaa ctg gcc Thr Arg Arg G u Leu Ala 80 gtt gcc gat aaa ccg ttt Val Ala Asp Lys Pro Phe 95 gtc gaa gcc gcc gaa aag Val G u Ala Ala G u Lys 110 agg cgc aac gac acc cac Arg Arg Asn Asp Thr His 125 ctt ggt gcg atg cgt gga Leu G y Ala IVet Arg y 140 145 gat tcc ttg gag gca ggt Asp Ser Leu G u Ala y 160 tca cac gta gtc gat cag Ser His Val Val Asp G n 175 gtc acc gcc caa ctt gga Val Thr Ala G n Leu y 190 gca agg ttc cgc atc gtg Ala Arg Phe Arg lie Val 205 att tct gcc agc aag att Ile Ser Ala Ser Lys Ile 220 225 gtg ggc gag cgc ggc tcc Val Gy u Arg Gy Ser 240 gtg gcg atc aaa cag gga Val Ala lle Lys Gn y 255 ggc tac gaa tcg gag gag G y Tyr u Ser G u G u 270 tca aag gtg att cct tca Ser Lys Val Ile Pro Ser 285 gcc ttt gcc ttg gct gtg Ala Phe Ala Leu Ala Val 300 305 cgt gaa ggt gtt gca gtg Ar G 3u yv Val Ala Val gat gca gtg gtg Asp Al a Val Val ttg Leu gca Al a gct Al a at t SIle 130 ct g Leu cct Pr o acc Thr t cc Ser ttg Leu 210 gac Asp t ac Tyr ctt Leu cgg Ar g gca Al a 290 gaa G u ct c Leu gaa G u cca Pr o gga G y 115 gt c Val gac Asp gaa G u ct g Leu at c SIle 195 gaa G u cgc Ar g gt a Val cga Ar g tgg Tr p 275 caa G n aac Asn aag Lys gca Al a t ca Ser 100 gt g Val acg Thr ct g Leu ggt G y gtt Val 180 gac Asp cat Hi s ttg Leu t cc Ser cca Pr o 260 ggc G y ggc G y ggt G y gt g Val at c I e gcc Al a ct g Leu gca Al a cga Ar g ggt G y 165 ct c Leu ctt Leu gaa G u gag G u aac Asn 245 acc Thr acc Thr gat Asp ggc G y ttg Leu 325 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 gca ggg ccg gtg cct gca Ala G y Pro Val Pro Al a 310 315 V J 320 Page 386 Gene Sequence 24- 2- 06. txt gat gct gta gcc cag agc gct gcg gaa aaa cgc acc att gag ttg agc Asp Ala Val Ala G n Ser Ala Ala u Lys Arg Thr Ile u Leu Ser 330 335 340 taaggagaag tgctgctggc tgc <210> 304 <211> 341 <212> PRT <213> Cor ynebact eri um gl ut ai cum 1123 1146 <400> 304 Met Thr II 1 Phe Hi s T Val Val Al Pro Asp V, Val Asp Al Al a Leu G Phe Al a Pr Lys Al a G 11 Hi s I I e V, 130 G y Leu A, 145 Sy Pro G G n Thr LE Sy Ser II 1 Val Leu G 210 Ile Asp Ar 225 Ser Tyr V, G y Leu Ar G u Arg Tr 2; e Arg hr Pro a Arg al Al a a Val u Al a ro Ser 100 y Val 15 al Thr sp Leu u y eu Val 180 e Asp 95 u Hi s rg Leu al Ser rg Pro 260 p G y 75 I e 5 Tyr Ser l Ie Val l Ie Al a Leu Al a Ar g Gy 165 Leu Leu G u G u Asn 245 Thr Thr G y Leu Val y G n Gy Gy Ser Al a Asp Asn Gy 135 Asp Leu Gy G u Gy 215 Tr p Thr Asp Val Al a Thr 40 Ser Thr Gy Al a Val 120 I le Leu Ar g Pr o Gy 200 Al a G u Asp Ar g Thr 280 Ser 25 Lys Leu Pr o Val et 105 Phe G n SIle Asp Al a 185 Pr o Val SIle Val G u 265 Asp Tyr 10 Thr Al a Thr Pr o Al a 90 G u Hi s G u G u Leu 170 Thr Thr Ser Ar g G n 250 Hi s Gy Hi s Al a G u Al a 75 Val Leu Asn G u Pr o 155 Gy Ser Asn Hi s Leu 235 Thr Tr p Gy G u Al a Leu Ar g Al a G u Ar g 125 Gy Ser Hi s Thr Ar g 205 Ser Gy Al a Tyr Lys 285 Gy Leu G u G u Ar g Asp Al a 110 Asn Al a Leu Val Al a 190 Phe Al a G u l Ie G u 270 Ar g Val Asp Leu G u Lys Al a Asp bet G u Val 175 G n Ar g Ser Ar g Lys 255 Ser Leu Gy Leu Gy Leu Pr o G u Thr Ar g Al a 160 Asp Leu SIe Lys Gy 240 G n G u G u G y Ser Val Ile Pro Page 387 ene Sequence 24- 2- 06. txt Ser Al a O31n 31y Asp Tyr Thr Ar g Phe Tyr Asp Ala Phe Ala Leu Ala 290 295 300 Val 31u As n (1y 1y Al a (1y Pr oVal Pr oAl aAr g (1u 1y Val Al a 305 310 315 320 Val Leu Lys Val Leu Asp Al a ValI Al a O31n Ser Ala Ala Ou Lys Ar g 325 330 335 Thr Ilie O31u Leu Ser 340 <210O> 305 <21 1> 1200 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 ODS <222> (1177) <223> RXN01630 <400> 305 gt aggt gagt ct t cgt gaga t acccccggc cagt cat aca ccagat aaaa acct gcgggt t gcgtt t tag gagaat tccc gttcaaccaa gctccaccac at g agt gat caa aaa 11! I'bt Ser Asp 03 n Lys at t I Ie gt g ValI g at As p ccc Pr o at g vb t ct c Le u gt g ValI c ag O31n c ag O31n ag c Ser gt t ValI gcc Al a g at As p gag O31u c ac Hi s gcc Al a g at As p at c I Ie 120 tt a Le u c aa O31n ct g Le u cgt Ar g cgc Ar g g at As p aag Ly s aaa Ly s gag O31u c ag O31n gag O31u cgc Ar g ct a Le u gaa O31u ct c Le u gac As p ag c Ser 75 t cc Ser gag O31u gt c ValI ct t Le u ggc (31y ggc O31y at t I Ie c ag O31n gt c ValI aag Ly s gt c ValI ct c Le u ggg O31y ct c Le u 140 gcc Al a ac c Thr ggg O31y tt c Ph e aac As n at g vb t gt g ValI gcc Al a 110 aac As n gcc Al a aaa Ly s c ac Hi s 15 gt a ValI ac c Thr g ac As p gt c ValI gag O31u 95 ct c Le u gt c ValI (31y ccg Pr o gag O31u g ac As p g at As p cct Pr o 80 aag Ly s aaa Ly s cgc Ar g ct c Le u gcg Al a gt c ValI t at Tyr 50 at c I Ie gcc Al a ggc O31y gct Al a t ct Ser 130 ggc (31y t cg Ser gcc Al a 35 g at As p aac As n aag Ly s ggg O31y gcc Al a 115 g aa O31u g aa (31u gcg Al a 20 gcc Al a gt t ValI gcc Al a cgc Ar g gga O31y 1 00 g at As p t cg Ser gt c ValI cgg Ar g gcg Al a g aa O31u at c I Ie gcg Al a ac a Thr cct Pr a gt t ValI gt c ValI gt a ggt gag cat at c acc gag Val Oy Ou His IlieThr Ou Page 388 Gene Sequence 24-2-06.txt 150 155 160 165 cac ctc aac caa ccc gca gac atg ggc ggt gtg ttg tgg att ctt ggc 643 His Leu Asn n Pro Ala Asp IV t G y G y Val Leu Trp lie Leu G y 170 175 180 tgc cac atg ctc gat gca ttg gtg gaa gtc ttc gga gct cca gaa tcc 691 Cys His MVt Leu Asp Al a Leu Val G u Val Phe G y Al a Pro G u Ser 185 190 195 gtg aac gcc cga gtg cat aag acc gca aaa ctc tct gac gac acc agc 739 Val Asn Ala Arg Val His Lys Thr Ala Lys Leu Ser Asp Asp Thr Ser 200 205 210 cgc gaa gac tca gcc tcc gca ctg ctg tac tac cca gat ttc tcc gtc 787 Arg G u Asp Ser Ala Ser Ala Leu Leu Tyr Tyr Pro Asp Phe Ser Val 215 220 225 agc ttc agc ttc gac ggc cac gat gat ctg gaa tgg ttc gaa agc tcc 835 Ser Phe Ser Phe Asp G y His Asp Asp Leu G u Trp Phe u Ser Ser 230 235 240 245 cga ctc acg gtc tat gga acc aag ggc atg atc gaa gcc gga atc ctc 883 Arg Leu Thr Val Tyr y Thr Lys y IV t lie u Ala y lie Leu 250 255 260 cct cag aca ctg cgc gta tac ctc aat gag tca cgc cag ggc tgg cca 931 Pro G n Thr Leu Arg Val Tyr Leu Asn u Ser Arg G n y Trp Pro 265 270 275 cag ggt tgg acc gag tgg acc cag agc tac ttc acc cca ccg ttt gct 979 G n y Trp Thr G u Trp Thr G n Ser Tyr Phe Thr Pro Pro Phe Ala 280 285 290 cgc aca gaa tcc aac aaa ttc tca gag ctt cca gag cta gaa aac atc 1027 Arg Thr u Ser Asn Lys Phe Ser G u Leu Pro G u Leu G u Asn Ile 295 300 305 agc aac ttc cgc aca gaa atg cag ggg tgg gtg aat tcc att cgc act 1075 Ser Asn Phe Arg Thr G u Iet G n Gy Trp Val Asn Ser lie Arg Thr 310 315 320 325 gga tcc cgc aat gtg gcg cca gtt gag gat gct ctc aca gtc gct cgc 1123 Sy Ser Arg Asn Val Al a Pro Val G u Asp Ala Leu Thr Val Ala Arg 330 335 340 att gtc agt gca tgc tac gaa tcc gac aac aac cag ggc att tcc gta 1171 lie Val Ser Ala Cys Tyr u Ser Asp Asn Asn n y Ile Ser Val 345 350 355 aac atc taagaggagc actccatgaa acc 1200 Asn I e <210> 306 <211> 359 <212> PRT <213> Cor ynebact eri um gl ut arr cum <400> 306 IVet Ser Asp G n Lys I e Val Val G y Leu Leu G y I e Thr His Pro 1 5 10 His Ala Ser Ala Arg Val Arg Ala Leu Arg G u ile Asp y Val O u 25 Val Val Ala Ala Ala Asp Thr Asp Ser Arg Leu G n Tyr Phe Thr Asp Page 389 ene Sequence 24- 2- 06. txt Ly s Ar g Hi s Pr o (31u Le u I Ie 145 (31u Le u G31y Ser Pr o 225 Tr p (31u Ar g Th r (31u 305 As n Le u O31n As p As n Ly s G31y A a 115 O31u Ou le le Pr o 1 95 As p Ph e O31u G31y G31y 275 Pr a Ou le ValI I Ie 355 Ou le A a Th r Pr a ValI ValI O31u 1 65 G31y Ser Ser ValI Ser 245 Le u Pr a A a I Ie Th r 325 Ar g ValI Ar g ValI A a A a Ar g Ar g 135 ValI Le u Hi s As n O31u 215 Ph e Le u O31n G31y Th r 295 As n Ser ValI I Ie O31u Hi s A a As p I Ie 120 Le u O31n As n vb t A a 200 As p Ser Th r Th r Tr p 280 O31u Ph e Ar g Ser Leu Asn Asp Asp I I e Asp Asp Ser G31y Le u 1 05 ValI Ly s A a O31n Le u 1 85 Ar g Ser Ph e ValI Le u 265 Th r Ser Ar g As n A a 345 Ser Ser O31u ValI Le u G31y 1 A a A a Hi s Ser G31y 235 G31y ValI Tr p Ly s O31u 315 A a Ty r As p ValI Le u Ty r 125 As p A a vb t ValI Th r 205 Le u As p Ly s Le u O31n 285 Ser O31n ValI Ser ValI A a 110 As n A a Ly s O31y O31u 1 A a Le u As p O31y As n 270 Ser O31u O31y O31u As p 350 ValI O31u Le u ValI O31y ValI O31y 1 ValI Ly s Ty r Le u vb t 255 O31u Ty r Le u Tr p As p 335 As n <210O> 307 <21 1> 1212 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn Page 390 Gene Sequence 24-2-06.txt <220> <221> CDS <222> (101)..(1189) <223> RXN00528 <400> 307 ttctgctggg aatccccaca ttttggaacg tagcgtcgat tcggtcgcgg ccgttatctt tttaagagga gaaattttag aagcgtgcgg cgaagctttt at c SIle cag G n ggt Gy ttc Phe gcc Al a gt c Val ct g Leu gt c Val t cc Ser gcc Al a 150 at c SIle cca Pr o cac Hi s cgc Ar g gtt Val gt g Val at g Net gcc Al a gcc Al a cag G n gat Asp gtt Val 120 ct c Leu at c l Ie t cc Ser gtt Val gt c Val 200 at g bet at t I e t at Tyr gt c Val ttc Phe gag G u ggc Gy t ac Tyr cag G n gt g Val gca Al a cct Pr o 170 gat Asp gca Al a ct c Leu gcc Al a tac Tyr aaa Lys gac Asp gct Al a 75 at c SIle cgc Ar g gcg Al a ggc Gy ggc Gy 155 gag G u gac Asp cgc Ar g aac Asn gga Gy cga Ar g ttc Phe gt c Val t ca Ser aac Asn gcg Al a ctt Leu t cc Ser 140 tgc Cys tgg Tr p at c SIle ctt Leu gt c Val 220 gt c Val aat Asn ggt Gy 45 gac Asp caa G n gt g Val acc Thr at c SIle 125 gaa G u gcc Al a gct Al a aaa Lys ttt Phe 205 ggc G y gga G y gcg Al a gat Asp gcc Al a aac Asn ct g Leu at c SIle 110 gac Asp gaa G u ttt Phe aag Lys t cc Ser 190 gaa gcu ggc Gy aac Asn 15 gat Asp t ac Tyr gaa Gu tgc Cys cgt Ar g 95 gac Asp gca Al a gcc Al a gt c Val aag Lys 175 cag G n gaa G u aac Asn tgc Cys cct Pr o cac Hi s aaa Lys act Thr 80 ggc Sy gag G u aaa Lys gac Asp aac Asn 160 ttc Phe at c SIle cgt Ar g at g bet at g Net 1 gcg Al a t cc Ser gtt Val gt a Val at c SIle ccg Pr o t cc Ser gcc Al a aaa Lys 145 gct Al a act Thr ggt G y ggc G y gac Asp 225 acc Thr gaa G u ggc G y ggc Gy aaa Lys act Thr acc Thr gat Asp 130 ttc Phe ct c Leu gac Asp gca Al a gtt Val 210 ttc Phe t cc Ser act Thr gac Asp at c SIle at c SIle ct c Leu gcc Al a 115 gtt Val t ac Tyr cca Pr o gct Al a acc Thr 195 cgc Ar g aaa Lys ct c Leu gt c Val at t SIle gat Asp gcc Al a gac Asp 100 gag G u ttg Leu gca Al a gt a Val ggc G y 180 at c SIle gt a Val aac Asn agc acg t cc Ser Thr Ser acc Thr att SIe ccg Pr o gaa G u ctt Leu gat Asp ggc Sy cca Pr o gtg Val caa G n ttc Phe 165 at c SIe acc Thr gat Asp at g bet Page 391 Gene Sequence 24-2-06.txt ctt Leu 230 gt g Val cgc Ar g cgc Ar g ccc Pr o gcc Al a 310 cgc Ar g t cc Ser gca Al a cgc Ar g t cc Ser gt c Val tgg Tr p 280 aac Asn at c l Ie at c SIle cct Pr o at c l Ie 360 aat cgc ttg gaa tcc Asn Arg Leu u Ser 235 aac att cca gat ggt Asn lie Pro Asp y 250 cac atc gga cca tcc His lie y Pro Ser 265 gct tat gtc cgc ctc Ala Tyr Val Arg Leu 285 ctt gag tac aaa ctc Leu G u Tyr Lys Leu 300 atc atc gac gct gtt Ile Ile Asp Ala Val 315 ggc gga ccg atc atg Gy G y Pro lie IVet 330 gag cag ctt cca gac Su n Leu Pro Asp 345 atc gag gcg taaaatt Ile G u Al a aag aag gt c Lys Lys Val tcc aaa acc caa Ser Lys Thr G n cca Pr o gac Asp 270 gaa G u gag G u cgc Ar g cca Pr o gat Asp 350 ct g Leu 255 cac Hi s ggc Gy gt g Val gcc Al a gca Al a 335 gtt Val 240 tct Ser gt c Val acc Thr tgg Tr p gcc Al a 320 agc Ser gct Al a gga Gy caa G n gca Al a gat Asp 305 aag Lys t cc Ser tgt Cys aag Lys tgg Tr p ttc Phe 290 t ca Ser at c SIle t ac Tyr gaa G u gaa G u 260 gat Asp gga Gy aac Asn ct c Leu at g Met 340 ct a Leu gca Al a 245 gac Asp gac Asp gtt Val t ct Ser gat Asp 325 aag Lys gag G u 835 883 931 979 1027 1075 1123 1171 1212 agg ctaaaaattt ggg <210> 308 <211> 363 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 308 Met Ser Thr Ser Thr IIe Arg Val Ala 1 5 Ala Thr Ser Leu I e n y Val u 25 Ser G u Thr Val Pro G y Leu et His 40 Val y Asp I e u Phe Val Ala Al a 55 Val Gy I e Asp Leu Al a Asp Ala Thr 70 Ile Lys lie Ala Asp Val Pro n Thr Pro Thr Leu Asp G y Leu G y Asp His 100 105 Ser Thr Ala G u Pro Val Asp Val Val 115 120 Ala Asp Val Leu Val Ser Tyr Leu Pro I e Al a G y 10 Tyr Tyr Arg Val Lys Phe Phe Asp Val G u Al a Ser G y Ile Asn 90 Tyr Arg Ala G n Al a Leu Val G y Ser Page 392 Val Asn Sy Asp G n Val Thr SIle 125 G u Sy Al a Asp Al a Asn Leu SIle 110 Asp G u Asn Asp Tyr G u Cys Ar g Asp Al a Al a Gene Sequence 24-2-06.txt 140 Cys Ala Phe Val Lys 145 Al a Thr Gy Gy Asp 225 Ser Gy G n Al a Asp 305 Lys Ser Cys Tyr Pr o Al a Thr 195 Ar g Lys Thr Val Leu 275 Gy Pr o Al a Leu Ar g 355 G n Phe 165 l le Thr Asp bet Al a 245 Asp Asp Val Ser Asp 325 Lys G u Al a 150 I le Pr o Hi s Ar g Leu 230 Val Ar g Ar g Pr o Al a 310 Ar g Ser Al a Al a Al a SIle Ar g Thr 215 Asp Thr Asn Lys Leu 295 Gy Gy Pr o Phe I le Asp Al a Ser Asp Pro 170 Val G y Asp 185 Val Leu Al a 200 IVet G n Leu Arg Asn Arg Ser Asn Il e 250 Val His Ile 265 Trp Al a Tyr 280 Asn Leu G u Ile lie lle lie y G y 330 Pro G u G n 345 Ile lie G u Gy 155 G u Asp Ar g Asn Leu 235 Pr o Gy Val Tyr Asp 315 Pr o Leu Al a Tr p SIle Leu Val 220 G u Asp Pr o Ar g Lys 300 Al a SIle Pr o Al a Lys Phe 205 Gy Ser Gy Ser Leu 285 Leu Val bet Asp Lys 175 G n G u Asn Lys Leu 255 Hi s Gy Val Al a Al a 335 Val <210> 309 <211> 795 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(772) <223> RXN03057 <400> 309 catcaacgcc gagtacaact aaggacaact gataatgaca aatgctgcaa ttgtcggatg aggagacgtc gcaaccgttc atacagaagc gctggaagct ttg gct tcc gat ctt 115 Leu Ala Ser Asp Leu 1 ggt att aag ttc gtc gca gtg gtg gat aaa gat cta gag act gct gag 163 Gy Ile Lys Phe Val Ala Val Val Asp Lys Asp Leu G u Thr Ala G u 15 aaa ttt gcg acg gga ctt gga gct gct ggc gat tct tca gaa agc agc 211 Lys Phe Al a Thr G y Leu G y Al a Al a G y Asp Ser Ser G u Ser Ser Page 393 Gene Sequence 24-2-06.txt gt c Val gat Asp ct c Leu gct Al a aac Asn gtt Val gcc Al a t ac Tyr 150 ggc Gy ttc Phe t at Tyr gcc Al a ct a Leu gcg Al a gag G u gat Asp t cc Ser 120 aat Asn acc Thr ct g Leu gga Gy gat Asp 200 ggc Gy at c SIle cac Hi s gac Asp cca Pr o gaa G u gca Al a aaa Lys aac Asn 170 gca Al a at c SIle agc Ser acc Thr cac Hi s 75 cag G n aag Lys ct g Leu t at Ty r cct Pr o 155 caa G n aca Thr gat Asp ct g Leu acc Thr ggt Gy gcg Al a at t SIle cgt Ar g t cc Ser 140 tgg Tr p gca Al a gaa G u gtt Val ccg Pr o 45 ccc Pr o gt a Val cag G n gca Al a cgt Ar g 125 tct Ser cgt Ar g at t l Ie gt c Val gaa G5u 205 gct Al a cac Hi s aat Asn cgt Ar g gt g Val 110 ct g Leu gt g Val ggc Gy cac Hi s aag Lys 190 gac Asp ttc Phe caa G n at c SIle 80 at c SIle t at Tyr gat Asp tgg Tr p caa G n 160 ct g Leu act Thr gca Al a t cc Ser cac Hi s ct g Leu gac Asp cag G n t ca Ser acc Thr 145 gca Al a gat Asp gt c Val cac Hi s aaa Lys at t SIle gaa G u t ac Tyr aac Asn ggt Sy 130 cgc Ar g cac Hi s ct g Leu t cc Ser gca Al a 210 aag Lys ttg Leu ccg Pr o gat Asp 100 t ac Tyr ct c Leu cca Pr o ggt G y cag G n 180 gat Asp at c l Ie at c SIe gct Al a ttg Leu gaa G u aac Asn ggt Sy ggc G y ggt G y 165 tgg Tr p aag Lys ggt G y cac gag tcc gga gtc cac His u Ser y Val His 215 aca <210> 310 <211> 224 <212> PRT <213> Corynebacter i um gl <400> 310 Leu Ala Ser Asp Leu G y 1 5 Leu G u Thr Ala G u Lys Ser Ser u Ser Ser Val acc Thr 220 agt gaa gtg agt Ser G u Val Ser tgaaccatgc tattggtgat 792 ut am cum Ile Lys Phe Val Ala Val Val Asp Lys Asp 10 Phe Al a Thr G y Leu y Ala Ala y Asp 25 Lys Ala His G y Ser Leu Pro Ala Leu Phe 40 Page 394 Gene Sequence 24- 2- 06. txt Leu His Ile Thr Thr Pro His Ser Lys His Il e Leu G u Asp Tyr Sn Asn Ser G y 130 Thr Arg 145 Al a Hi s Asp Leu Val Ser Hi s Al a Lys Lys lie Asp Val Asp n Gy Lys Leu Ar g 115 Asp Thr Ser Leu Thr 195 Tyr Al a Leu G u Asn Gy Gy Gy 165 Tr p Lys Gy Leu 70 Al a Asn Val Al a Tyr 150 Gy Phe Tyr Hi s G u Asn Pr o Ser l Ie 135 Tyr Leu Leu Al a G u 215 Al a G u Asp Ser 120 Asn Thr Leu Gy Asp 200 Ser Leu Leu Gy 105 G n Gy G n Met Lys 185 Val Gy Hi s Asp 90 Pr o G u Al a Lys Asn 170 Al a SIle Val Hi s G n Lys Leu Tyr Pr o 155 G n Thr Asp Hi s Sy Al a SIle Ar g Ser 140 Tr p Al a G u Val Thr 220 Val G n Al a Ar g 125 Ser Ar g l Ie Val G u 205 Ser Val Leu Cys Leu Val G n Thr 175 Sy Thr Val <210> 311 <211> 795 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(772) <223> FRXA02902 <400> 311 catcaacgcc gagtacaact aaggacaact gataatgaca aggagacgtc gcaaccgttc atacagaagc gctggaagct ggt Sy aaa Lys gt c Val gat Asp aag Lys gcg Al a gcc Al a ct a Leu gt c Val gga Sy ggc Sy at c SIle gca Al a ctt Leu agc Ser acc Thr gt g Val gga Sy ct g Leu acc Thr gt g Val gct Al a ccg Pr o 45 ccc Pr o gat Asp gct Al a gct Al a cac Hi s aaa gat Lys Asp 15 ggc gat Sy Asp ttg ttc Leu Phe gac caa Asp n Page 395 aat gct gcaa t t gt cggat g ttg gct tcc gat ctt 115 Leu Ala Ser Asp Leu 1 cta gag act gct gag 163 Leu G u Thr Ala G u tct tca gaa agc agc 211 Ser Ser G u Ser Ser tcc aaa aag aag atc 259 Ser Lys Lys Lys Ile cac att ggt ttg gct 307 Hi s i e G y Leu Al a Gene Sequence 24-2-06.txt ct c Leu gct Al a aac Asn gtt Val gcc Al a t ac Tyr 150 ggc Gy ttc Phe t at Tyr gcg Al a gag G u gat Asp t cc Ser 120 aat Asn acc Thr ct g Leu gga Gy gat Asp 200 cac Hi s gac Asp cca Pr o gaa G u gca Al a aaa Lys aac Asn 170 gca Al a at c SIle cac Hi s 75 cag G n aag Lys ct g Leu t at Ty r cct Pr o 155 caa G n aca Thr gat Asp ggt Gy gcg Al a at t SIle cgt Ar g t cc Ser 140 tgg Tr p gca Al a gaa G u gtt Val gt a Val cag G n gca Al a cgt Ar g 125 tct Ser cgt Ar g at t l Ie gt c Val gaa G5u 205 aat Asn cgt Ar g gt g Val 110 ct g Leu gt g Val ggc Gy cac Hi s aag Lys 190 gac Asp at c I e 80 at c SIle t at Tyr gat Asp tgg Tr p caa G n 160 ct g Leu act Thr gca Al a ctg gaa Leu G u gac t ac Asp Tyr cag aac Sn Asn t ca ggt Ser G y 130 acc cgc Thr Arg 145 gca cac Al a Hi s gat ctg Asp Leu gtc t cc Val Ser cac gca Hi s Al a 210 aag ccg ttg Lys Pro Leu ttg gat gaa Leu Asp G u 100 cgt tac aac Arg Tyr Asn 115 gac ctc ggt Asp Leu G y acc cca ggc Thr Pro y t cc ggt ggt Ser G y G y 165 ctg cag tgg Leu G n Trp 180 acc gat aag Thr Asp Lys 195 t ac at c ggt Tyr Ile y cac gag tcc gga gtc cac His u Ser y Val His 215 aca <210> 312 <211> 224 <212> PRT <213> Corynebacter i um gl <400> 312 Leu Ala Ser Asp Leu G y 1 5 Leu G u Thr Ala G u Lys Ser Ser G u Ser Ser Val Ser Lys Lys Lys Ile Asp Hi s lie G y Leu Ala Leu 70 Leu G u Lys Pro Leu Al a acc Thr 220 agt gaa gtg agt Ser G u Val Ser tgaaccatgc tattggtgat 792 ut am cum I e Lys Phe Phe Al a Thr 25 Lys Al a Hi s 40 Val Leu Hi s G u Al a Leu Asn G u Leu Val Ala 10 G y Leu Sy Ser I I e Thr Hi s His Asp Gn 90 Page 396 Val Al a Pr o Pr o Val G n Asp Al a Al a Hi s Asn Ar g Lys Gy Leu Asp Val Leu Asp G n Ser Thr 145 Al a Asp Val Hi s Leu Ar g 115 Asp Thr Ser Leu Thr 195 Tyr G u Asn Gy Gy Gy 165 Tr p Lys Gy Asn Val Al a Tyr 150 Gy Phe Tyr Hi s Pr o Ser l Ie 135 Tyr Leu Leu Al a G u 215 Gene Sequence 24-2-06.txt Asp Gy Pro Lys lie Ala Val 105 110 Ser n G u Leu Arg Arg Leu 120 125 Asn G y Al a Tyr Ser Ser Val 140 Thr n Lys Pro Trp Arg Gy 155 Leu IVet Asn G n Ala lie His 170 Sy Lys Ala Thr G u Val Lys 185 190 Asp Val lie Asp Val G u Asp 200 205 Ser y Val His Thr Ser G u 220 Cys Leu Val G n Thr 175 Sy Thr Val Tyr Asp Tr p G n 160 Leu Thr Al a Ser <210> 313 <211> 831 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(808) <223> RXA00251 <400> 313 aaccagcgtt ttcagcgaga tactggacat atc acatgggttt tatacagaaa attcatacga aag gcg Al a gac Asp cat Hi s gat Asp aac Asn gac Asp gtt Val t cc Ser gca Al a gt g Val gac Asp acc Thr gga Sy gac Asp ct c Leu gaa G u gt g Val gaa G u gcg Al a cac Hi s gca Al a gt g Val gat Asp 75 gcc Al a acc Thr at t SIle gag G u ttg Leu acg Thr ggc G y gga Sy gt c Val at c SIle 45 gaa G u ct g Leu agt Ser ggc Sy t ac Tyr gag G u gag G u gt g Val gt g Val aactaaa atccctgaat aaaacatcta igttgatc atg aag aag aag att 115 IVet Lys Lys Lys Ile 1 atg gga att gag atc gtc aaa 163 IVet y Ile u Ile Val Lys 15 gcc ttg ggc cga aat cca gag 211 Al a Leu G y Arg Asn Pro G u gga gta gag cct atc gag tcc 259 Sy Val u Pro Ile u Ser gga ggc gtc gac aag cta aaa 307 Sy y Val Asp Lys Leu Lys cac gcc gcg gcg gtg gcg cgt 355 His Ala Ala Ala Val Ala Arg 80 gcc gaa tgg cac gca cac ctt 403 Ala G u Trp His Ala His Leu Page 397 Gene Sequence 24-2-06.txt gat ctc aac gtc att gtc ccg Asp Leu Asn Val lie Val Pro 105 gcc ctc cgc gcg gca tcc ggc Ala Leu Arg Ala Ala Ser y 120 ggc aac gga cca cac ccc ggc G y Asn G y Pro His Pro G y 135 140 gcc ctc cgc gga ctc gcc gac Ala Leu Arg y Leu Al a Asp 150 155 ggc atc cgc gtc agc act gtc Gy lie Arg Val Ser Thr Val 170 ctg caa ggc ctc atg gac t ca Leu G n y Leu IVet Asp Ser 185 tac atc gaa cca aaa gaa atc Tyr lie u Pro Lys Gu Ile 200 gct ggc gaa acc acc cag atc Al a y G u Thr Thr G n Ile 215 220 gaa ctg gcg gac cgg aaa gat G u Leu Ala Asp Arg Lys Asp 230 235 <210> 314 <211> 236 <212> PRT <213> Cor ynebact er i um gl ut a <400> 314 IVet Lys Lys Lys Ile Ala Val 1 5 lie Gu lie Val Lys Asp Leu G y Arg Asn Pro G u His Leu Su Pro lie Gu Ser Asp lie Val Asp Lys Leu Lys Asn Leu 70 Ala Ala Val Ala Arg Asp Thr Trp His Ala His Leu Asp Leu 100 Arg G n Leu Leu Pro Ala Leu 115 gcc Al a tgc Cys 125 aac Asn gcc Al a agc Ser caa G n gcc Al a 205 acc Thr gag Su 110 gt c Val acc Thr ttc Phe ccc Pr o ggc Sy 190 aac Asn aac Asn ttg Leu at c SIle at c SIle cgc Ar g ggc Sy 175 acc Thr gca Al a gt g Val agt Ser t ac Tyr t ac Tyr aaa Lys 160 ccc Pr o aac Asn at c l Ie gac Asp cgc Ar g at c SIle gcc Al a 145 gaa G u acc Thr ttc Phe aga Ar g gt a Val 225 caa G n aac Asn 130 gcc Al a gaa G u aac Asn cgc Ar g ttc Phe 210 cga Ar g ctc ttg Leu Leu 115 tcc ggc Ser G y agc aaa Ser Lys gcc aac Al a Asn acc ccc Thr Pro 180 cca gag Pro G u 195 gtg att Val lie cca cgt Pro Arg tagttctggg gggcttcctg ggc ri cum Val Thr Ser Arg Al a Al a 40 Val Lys Asp Hi s Thr Ile Asn Val 105 Arg Ala 120 G y Al a 10 Asp His I Leu Al a Su Val I Val Asp 75 G u Al a 90 Ile Val I Al a Ser Page 398 Sy Val SIle G u Leu Ser Al a Cys 125 et Al a Sy Sy Hi s Al a Leu SI e Sy Leu Val Sy Al a G u Ser Tyr Gene Sequence 24 IIe Asn Ser Gy Ala G y Asn Gy Pro His Pro 130 135 Ala Ala Ser Lys His Al a Leu Arg G y Leu Ala 145 150 155 Su u Ala Asn Asn Gy lie Arg Val Ser Thr 165 170 Thr Asn Thr Pro IVet Leu G n y Leu IVet Asp 180 185 Phe Arg Pro u lie Tyr lie u Pro Lys u 195 200 Arg Phe Val lie Asp Al a G y G u Thr Thr G n 210 215 Val Arg Pro Arg Ile G u Leu Ala Asp Arg Lys 225 230 235 <210> 315 <211> 1008 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(985) <223> RXN02654 <400> 315 tattttcgga aatttataca gcaatcctcg aaatcctaat gaggtacggt agttcgttcg aggacaacgt cgagaaaggc -2-06. txt G y Asn Thr 140 Asp Al a Phe Val Ser Pro Ser G n G y 190 li e Al a Asn 205 Ile Thr Asn 220 Asp SIe Ar g Gy 175 Thr Al a Val aaagatccct tatcgtggga aat Asn cag G n ggt Gy ctt Leu t at Tyr caa G n aaa Lys cca Pr o gaa G u t cc Ser act Thr cgc Ar g gat Asp ttt Phe acg Thr ggc Sy t at Tyr ggc Sy ggg Sy gac Asp ttc Phe ct a Leu ct a Leu caa G n gat Asp gca Al a 75 aga Ar g cct Pr o ttc Phe gat Asp gga G y tct Ser gat Asp gt g Val ggt G y ccg Pr o at a SIle agt Ser 45 ggg G y gtt Val ct c Leu gat Asp aaa Lys aaa Lys gga G y at t SIle gcg Al a caa G n ct c Leu 110 gt c Val 15 ctt Leu agg Ar g gga G y at c l Ie gca Al a 95 cgt Ar g gat Asp t cc Ser ctt Leu gct Al a gct Al a 80 at c l Ie gat Asp at g et 1 ccc Pr o ccc Pr o aag Lys gcc Al a t ac Tyr gag G u cca Pr o att tca ttg cta Ile Ser Leu Leu cca aag caa agc Pro Lys G n Ser caa gcc gat att G n Ala Asp Ile ggc cgc aag gct Sy Arg Lys Ala gt a gca at c gct Val Ala lle Ala ttg ccc gaa gaa Leu Pro G u G u gaa aca ggt caa G u Thr G y G n 100 gaa tac tgt cgc G u Tyr Cys Arg 115 Page 399 Gene Sequence 24-2-06.txt tcg ctg gtc caa gag acg gtg aac gct tta ggt ggc Ser Leu Val n u Thr Val Asn Ala Leu 3 y y cta gac atc ttg Leu Asp Ile Leu gt c Val acc Thr 150 ttt Phe at a SIle ttg Leu ttg Leu cca Pr o 230 gag u cac Hi s gcc Al a 120 aac aac Asn Asn 135 gac gaa Asp u cgg gtt Arg Val atc ttt Ile Phe gat tac Asp Tyr 200 gca agt Al a Ser 215 ggt cct G y Pro aaa at a Lys II e cct gt t Pro Val agc t at Ser Tyr 280 t ca Ser ttc Phe aaa Lys 170 tcg Ser at g Net ct g Leu tgg Tr p gga Gy 250 ttg Leu gt a Val cgt Ar g gac Asp 155 gca Al a t cc Ser act Thr at a SIle acg Thr 235 ttt Phe gca Al a gga Sy cag n 140 cag n gct Al a at t SIle aag Lys ggc (3G y 220 ccg Pr o ggc 3 y ggt G y gaa u 125 gt g Val act Thr at a l Ie cag n gcg Al a 205 gat Asp ttg Leu cag n gcg Al a acc Thr 285 tgg gca cct ggt Trp Al a Pro y 145 ttg cag gtt aac Leu n Val Asn 160 cct cat ctg aag Pro His Leu Lys 175 gcg tac caa cct Ala Tyr n Pro 190 gca ttg aac aat Ala Leu Asn Asn ggc att cgg gta 3 y lie Arg Val 225 caa ccc agc cat n Pro Ser His 240 cac gct ccg at t His Ala Pro Ile 255 tac gtt ttt ctc Tyr Val Phe Leu 270 ctg gga gtc aca Leu y Val Thr 130 ttg Leu ct c Leu ccc Pr o tcg Ser ttg Leu 210 aat Asn ggt Sy gga Sy gct Al a ggt Sy 290 acc Thr t at Tyr gga G y gaa (3 u 195 t ca Ser tct Ser cag G n aga Ar g tct Ser 275 gaa u ggt G y t ca Ser 180 acc Thr aag Lys gt a Val cca Pr o gcg Al a 260 gac Asp att SIe agt Ser 165 tcg Ser ct c Leu ggc G y gcc Al a caa G n 245 ggt Sy gaa u ggg acg ccc y Thr Pro acc cca tagtcggtac aagcg Thr Pro 295 <210> 316 <211> 295 <212> PRT <213> Corynebacter i um gl <400> 316 IVet Ile Ser Leu Leu Asn 1 5 Pro Pro Lys G n Ser G n Pro n Ala Asp Ile y Lys y Arg Lys Ala Leu Ala Val Ala lle Ala Tyr gaat c act 1008 ut ari cum Asp Pro Arg Pro 3 u Pro 25 Leu Ser Ser 40 I le Thr y 55 Al a Ar g u Thr Leu Phe Pro Lys Val Asp 10 y Leu Asp I e Lys Leu Ser Tyr G n y Ser y Arg Leu G y Asp Ser G y lie G y Al a y Ala Asp Val Ala I e Ala Page 400 Gene Sequence 24-2-06.txt 70 75 Tyr Leu Pro G u G u n Ala Asp Ala Asp Arg Val Leu n Ala lle 90 G u G u Thr G y G n Lys Al a Phe Ser Phe Pro G y Asp Leu Arg Asp 100 105 110 Pro G u Tyr Cys Arg Ser Leu Val n G u Thr Val Asn Ala Leu y 115 120 125 Sy Leu Asp Ile Leu Val Asn Asn Ala Ser Arg G n Val Trp Ala Pro 130 135 140 Sy Leu Thr G u lie Thr Asp G u Asn Phe Asp G n Thr Leu G n Val 145 150 155 160 Asn Leu Tyr G y Ser Phe Arg Val Thr Lys Ala Ala lie Pro His Leu 165 170 175 Lys Pro y Ser Ser Ile lie Phe Thr Ser Ser lie G n Ala Tyr G n 180 185 190 Pro Ser G u Thr Leu Leu Asp Tyr Ala Iet Thr Lys Ala Ala Leu Asn 195 200 205 Asn Leu Ser Lys y Leu Ala Ser Ser Leu Ile y Asp y lie Arg 210 215 220 Val Asn Ser Val Ala Pro y Pro Phe Trp Thr Pro Leu G n Pro Ser 225 230 235 240 His G y G n Pro n G u Lys IIe G u G y Phe G y G n His Ala Pro 245 250 255 IIe G y Arg Ala G y His Pro Val G u Leu Ala G y Ala Tyr Val Phe 260 265 270 Leu Ala Ser Asp G u Al a Ser Tyr Val Val y G u Thr Leu y Val 275 280 285 Thr y y Thr Pro Thr Pro 290 295 <210> 317 <211> 1008 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(985) <223> FRXA02654 <400> 317 tattttcgga aatttataca gcaatcctcg aaatcctaat aaagatccct tatcgtggga gaggtacggt agttcgttcg aggacaacgt cgagaaaggc atg att tca ttg cta 115 IVet I e Ser Leu Leu 1 aat gat cca cgt acg cta ttc ccg aaa gtc gat ccc cca aag caa agc 163 Asn Asp Pro Arg Thr Leu Phe Pro Lys Val Asp Pro Pro Lys G n Ser 15 cag ccg gaa cca ggc cta gat ata aaa ctt tcc ccc caa gcc gat att 211 On Pro u Pro y Leu Asp Ile Lys Leu Ser Pro n Ala Asp Ile Page 401 Gene Sequence 24-2-06.txt 30 ggt ctc tcc agc tat caa gga agt gga agg ctt aag ggc cgc aag gct 259 Sy Leu Ser Ser Tyr G n y Ser y Arg Leu Lys y Arg Lys Ala 45 ctt att act ggt ggc gat tct ggg att gga gct gcc gta gca atc gct 307 Leu Ile Thr G y y Asp Ser y Ile G y Ala Ala Val Ala Ile Ala 60 tat gct cgc gag ggg gca gat gtt gcg atc gct tac ttg ccc gaa gaa 355 Tyr Ala Arg G u G y A a Asp Val Ala Ile Ala Tyr Leu Pro G u G u 75 80 caa gcc gat gct gac aga gtg ctc caa gca atc gag gaa aca ggt caa 403 Sn Ala Asp Ala Asp Arg Val Leu Gn Ala lle G u u Thr G y n 95 100 aaa gct ttt tct ttc cct ggt gat ctc cgt gat cca gaa tac tgt cgc 451 Lys Al a Phe Ser Phe Pro G y Asp Leu Arg Asp Pro G u Tyr Cys Arg 105 110 115 tcg ctg gtc caa gag acg gtg aac gct tta ggt ggc cta gac atc ttg 499 Ser Leu Val n G u Thr Val Asn Ala Leu y y Leu Asp Ile Leu 120 125 130 gtc aac aac gcg tca cgt cag gtg tgg gca cct ggt ttg acc gaa att 547 Val Asn Asn Ala Ser Arg G n Val Trp Ala Pro y Leu Thr G u Ile 135 140 145 acc gac gaa aac ttc gac cag act ttg cag gtt aac ctc tat ggt agt 595 Thr Asp G u Asn Phe Asp G n Thr Leu G n Val Asn Leu Tyr y Ser 150 155 160 165 ttt cgg gtt acc aaa gca gct ata cct cat ctg aag ccc gga tca tcg 643 Phe Arg Val Thr Lys Al a Ala lie Pro His Leu Lys Pro G y Ser Ser 170 175 180 ata atc ttt aca tcg tcc att cag gcg tac caa cct tcg gaa acc ctc 691 lie lie Phe Thr Ser Ser lie G n Ala Tyr G n Pro Ser G u Thr Leu 185 190 195 ttg gat tac gcc atg act aag gcg gca ttg aac aat ttg tca aag ggc 739 Leu Asp Tyr Ala IVet Thr Lys Ala Ala Leu Asn Asn Leu Ser Lys y 200 205 210 ttg gca agt agt ctg ata ggc gat ggc att cgg gta aat tct gta gcc 787 Leu Ala Ser Ser Leu Ile y Asp y lie Arg Val Asn Ser Val Ala 215 220 225 cca ggt cct ttc tgg acg ccg ttg caa ccc agc cat ggt cag cca caa 835 Pro G y Pro Phe Trp Thr Pro Leu G n Pro Ser His G y G n Pro G n 230 235 240 245 gag aaa ata gaa gga ttt ggc cag cac gct ccg att gga aga gcg ggt 883 G u Lys IIe G u G y Phe G y G n His Ala Pro lle G y Arg Ala G y 250 255 260 cac cct gtt gag ttg gca ggt gcg tac gtt ttt ctc gct tct gac gaa 931 His Pro Val G u Leu Al a G y Ala Tyr Val Phe Leu Ala Ser Asp G u 265 270 275 gcc agc tat gtg gta gga gaa acc ctg gga gtc aca ggt ggg acg ccc 979 Ala Ser Tyr Val Val G y G u Thr Leu y Val Thr y y Thr Pro 280 285 290 acc cca tagtcggtac aagcggaatc act 1008 Thr Pro Page 402 Gene Sequence 24-2-06.txt 295 <210> 318 <211> 295 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 318 lIet Ile Ser Leu 1 Pro Pro Lys G n Pro On Ala Asp Lys y Arg Lys Ala Val Ala Ile Tyr Leu Pro G u G u G u Thr G y 100 Pro G u Tyr Cys 115 G y Leu Asp Il e 130 G y Leu Thr G u 145 Asn Leu Tyr G y Lys Pro y Ser 180 Pro Ser G u Thr 195 Asn Leu Ser Lys 210 Val Asn Ser Val 225 His G y G n Pr o lie y Arg Ala 260 Leu Al a Ser Asp 275 Thr G y G y Thr 290 <210> 319 Leu 5 Ser SIle Al a Al a G u G n Ar g Leu SIle Ser 165 Ser Leu Sy Al a G n 245 G y G u Pr o Asn Asp Pro Arg G n Sy Leu Ty r 70 G n Lys Ser Val Thr 150 Phe SIle Leu Leu Pr o 230 G u Hi s Al a Thr Pr o Leu SIle Al a Al a Al a Leu Asn 135 Asp Ar g SIle Asp Al a 215 Sy Lys Pr o Ser Pr o 295 Pr o 25 Ser Sy G u Al a Ser 105 G n Al a Asn Thr Thr 185 Al a Ser Phe G u G u 265 Val Leu Leu G n Asp Al a Ar g Pr o Thr Ar g Asp 155 Al a Ser Thr SIle Thr 235 Phe Al a Sy Pr o SIle Ser Sy Val Leu Asp Asn 125 Val Thr l Ie G n Al a 205 Asp Leu G n Al a Thr 285 Lys Lys Sy SIle Al a G n Leu 110 Al a Tr p Leu Pr o Al a 190 Al a Sy G n Hi s Tyr 270 Leu Val Leu Ar g Gy l e Al a Ar g Leu Al a G n Hi s 175 Tyr Leu SIe Pr o Al a 255 Val Sy Page 403 Gene Sequence 24-2-06.txt <211> 1605 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1582) <223> RXN01049 <400> 319 aagcacagca at t gagcaat actcccatgc atgttttcgc gtgatcacgc tatatcct t a aagaatattc tttattagtc agacctttaa aggaaacctt aca Thr at g Met ggc Gy acc Thr at c SIle at c l Ie tta Leu t ac Tyr at c SIle t cc Ser 150 gag G u ttc Phe t cc Ser at t SIle cca Pr o gag G u t ca Ser gat Asp ttg Leu gat Asp 120 gag G u cac Hi s aac Asn ctt Leu cct Pr o t cc Ser aaa Lys gtt Val at t SIle at c SIle gat Asp cgt Ar g gcc Al a tcg Ser gcc Al a 170 ggc Gy gat Asp gca Al a acc Thr acc Thr ggc Gy gct Al a gaa G u gca Al a cac Hi s 140 ttg Leu t at Tyr acc Thr gac Asp t ca Ser aag Lys 45 at t SIle at t SIle gt c Val ggc Gy cag G n 125 ggc Gy ct g Leu gt g Val gga Gy acc Thr 205 t ca cgt Ser Arg 15 cga ggt Arg G y cag cgc G n Ar g gat gct Asp Al a ttg aac Leu Asn gcg cta Al a Leu 95 aat gcg Asn Ala 110 tat gt g Tyr Val cgc acc Arg Thr tgg ctg Trp Leu atg acc Net Thr 175 atg gct Net Ala 190 ggc gaa G y G u gga Gy gga Gy gaa Gu gac Asp gcg Al a 80 gat Asp ct c Leu gag G u ggc Gy aaa Lys 160 at c SIle act Thr ctt Leu atg gga tca att cca IVet Gy Ser Ile Pro 1 cct tat gtc ctt gct Pro Tyr Val Leu Ala ctt tat gat gct tcc Leu Tyr Asp Ala Ser tcc cat gaa ttc acc Ser His G u Phe Thr cag gtg gtt tcg gag G n Val Val Ser G u gct gat cat cac aac Ala Asp His His Asn tct ttt gca tcc tca Ser Phe Ala Ser Ser 100 acc ccg tgc att acc Thr Pro Cys lie Thr 115 cag ctg cgc gcg gaa G n Leu Arg Ala G u 130 gtc tgc ttg cac acc Val Cys Leu His Thr 145 act gag ttc gag aaa Thr G u Phe G u Lys 165 ggt gag tac gtc tac Gy G u Tyr Val Tyr 180 tcg att gcc gcg tgg Ser Ile Ala Ala Trp 195 gat ctg act atc ttg Asp Leu Thr lie Leu 210 agt ggc att Ser G y li e 200 ttg gac gcc cat Leu Asp Al a Hi s Page 404 Gene Sequence 24-2-06.txt gag cac atc ggt gtt gat ccg gct Su His IIe y Val Asp Pro Ala ctg ttc ggt gag Leu Phe G y G u at c aga aac cct lie Arg Asn Pro gat Asp 230 ct g Leu aac Asn gct Al a at c SIle gtt Val 310 cgc Ar g ccc Pr o t cc Ser gaa G u gca Al a 390 gcc Al a gaa G u ct g Leu gag G u acg Thr 470 cca Pr o gaa G u ggc Gy t cc Ser 280 tct Ser ggc Gy at t SIle gaa G u ggc Gy 360 acc Thr t cc Ser gaa G u ct c Leu at g let 440 ct c Leu cag G n acc Thr cct Pr o 250 ggc Gy gcc Al a ct g Leu ct c Leu aag Lys 330 acc Thr gca Al a cct Pr o cag G n gt c Val 410 at g Net cgc Ar g cca Pr o cgc Ar g gat Asp 235 tgg Tr p gcc Al a at g bet tgg Tr p aac Asn 315 cct Pr o cca Pr o gcc Al a gaa G u cgc Ar g 395 at c SIle ctt Leu gcc Al a ggc Gy ttt Phe 475 220 gcc Al a ttc Phe gt g Val cgc Ar g tgt Cys 300 gac Asp gaa G u gct Al a t ca Ser cac Hi s 380 gtt Val gca Al a t cc Ser acc Thr ggc Gy 460 gcg Al a aaa Lys cat Hi s gat Asp gt g Val 285 t ac Tyr gt c Val aac Asn gt c Val gcg Al a 365 ttg Leu tgg Tr p t ca Ser gac Asp ct c Leu 445 acg Thr cac Hi s gtt Val gcc Al a tct Ser 270 at c SIle cgc Ar g gga Gy ct c Leu ct g Leu 350 cag G n tgg Tr p gaa G u gga Gy gcc Al a 430 cgc Ar g cgc Ar g cat Hi s gt c Val at t l Ie 255 aaa Lys ctt Leu gtt Val cgc Ar g gac Asp 335 ccg Pr o gcc Al a cgc Ar g cac Hi s cga Ar g 415 ct c Leu ggc Gy gcg Al a t ac Tyr gac Asp 240 cca Pr o acc Thr ccg Pr o t cc Ser gcc Al a 320 gaa G u ttc Phe acg Thr ggc Gy at g et 400 gt c Val gac Asp acc Thr acg Thr t cc Ser 480 225 aaa Lys gac Asp gt c Val agc Ser cgc Ar g 305 gt c Val gt g Val ttc Phe at c SIle gtt Val 385 ggg Sy t cc Ser acc Thr gca Al a cca Pr o 465 aag Lys aag Lys ggc Gy gca Al a gtt Val 290 gac Asp acc Thr ct g Leu t cc Ser acc Thr 370 ttc Phe aaa Lys acc Thr cca Pr o ctt Leu 450 cca Pr o gca Al a tgg Tr p tgg Tr p gt c Val 275 ccc Pr o cag G n tgg Tr p at c SIle ggg Gy 355 aac Asn gaa G u gcc Al a gac Asp gt c Val 435 at c SIle ttc Phe aga Ar g aag Lys cct Pr o 260 gcc Al a gaa G u tgc Cys ct g Leu cgc Ar g 340 gaa G u at t SIle gcc Al a ggc Sy cac Hi s 420 at c SIle gt c Val ggc ggy gag G u cac Hi s 245 t cc Ser gcc Al a cag G n at c SIe gaa Gu 325 gaa G u cgc Ar g cag G n ct c Leu gca Al a 405 cca Pr o cct Pr o ctt Leu acg Thr ctt Leu 485 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 Page 405 Gene Sequence 24- 2- 06. txt ttc gac gcc ctc tac ctc aag ttg gtc tagcttttcg cagtggaacg Phe Asp Ala Leu Tyr Leu Lys Leu Val 490 cgc <210> 320 <211> 494 <212> PRT <213> Corynebacter i um gl ut ai cum 1602 1605 <400> 320 eVt G y Ser 1 Pro Tyr Val Leu Tyr Asp Ser His G u G n Val Val Al a Asp Hi s Ser Phe Al a Thr Pro Cys 115 G n Leu Ar g 130 Val Cys Leu 145 Thr G u Phe G y G u Tyr Ser Ile Ala 195 Asp Leu Thr 210 Su lie Arg 225 Lys Lys Trp Asp Gy Trp Val Al a Val 275 Ile Pro 5 Leu Al a Al a Ser Phe Thr Ser G u His Asn Ser Ser 100 Ile Thr Al a u Hi s Thr 0 u Lys 165 Val Tyr 180 Al a Tr p I e Leu Asn Pro Lys Hi s 245 Pro Ser 260 Al a Al a Thr IVet Ser Ile Pro 10 IVet Asp Ile y Ser 25 Gy Cys Pro lie Lys 40 Thr G y G u G y Val 55 Ile Thr Ser Val I e 70 l e Lys Asp n Il e 90 Leu Ile Leu Val Asp 105 Tyr Ala Asp Ser Arg 120 l e Asp G u Lys Ala 135 Ser Tyr His Pro Ser 150 G u Phe Asn Lys Ala 170 Phe Lys Leu Al a G y 185 Ser G y lie Leu Asp 200 G u Hi s I I e G y Val 215 Asp G u Pro Ala Thr 230 Leu u u lie Pro 250 Asn lie y Pro y 265 Al a Thr Ser G y Al a 280 Phe Thr Gy Ser Asn 75 Al a Gy Ser Tyr Ar g 155 Lys SIle Al a Asp Asp 235 Tr p Al a bet Asp Al a Thr Thr Gy Al a G u Al a Hi s 140 Leu Tyr Thr Hi s Pr o 220 Al a Phe Val Ar g Asp Ser Lys I le SIle Val Gy G n 125 Gy Leu Val Gy Thr 205 Al a Lys Hi s Asp Val 285 Ser Ar g G n Asp Leu Al a Asn 110 Tyr Ar g Tr p et et 190 Gy Leu Val Al a Ser 270 I le Ar g Gy Ar g Al a Asn Leu Al a Val Thr Leu Thr 175 Al a G u Phe Val l Ie 255 Lys Leu Gy Gy G u Asp Al a Asp Leu G u Gy Lys 160 I e Thr Leu Gy Asp 240 Pr o Thr Pr o Ser Val Pro u n Ile Pro Ser Gy Leu Trp Cys Tyr Arg Val Ser Page 406 Gene Sequence 24-2-06.txt Ar g 305 Val Val Phe SIle Val 385 Gy Ser Thr Al a Pr o 465 Lys G n Tr p SIle Sy 355 Asn G u Al a Asp Val 435 l le Phe Ar g SIle G u 325 G u Ar g G n Leu Al a 405 Pr o Pr o Leu Thr Val 310 Ar g Pr o Ser G u Al a 390 Al a G u Leu G u Thr 470 295 Gy Thr Leu SIle G n 375 Leu Pr o Phe G u G n 455 Hi s 300 Asp Val Gy Arg Ala Leu Asn 315 Ile Lys Pr o 330 Sy Thr Pro 345 Trp Al a Al a G y Pro G u Tyr G n Arg 395 Arg Val lle 410 Ala IVet Leu 425 Lys Arg Ala G u Pro G y Pro Arg Phe 475 u Al a Ser Hi s 380 Val Al a Ser Thr Gy 460 Al a Asn Val Al a 365 Leu Tr p Ser Asp Leu 445 Thr Hi s Asp 335 Pr o Al a Ar g Hi s Ar g 415 Leu Gy Al a Tyr Al a 320 G u Phe Thr Gy bet 400 Val Asp Thr Thr Ser 480 G u Leu 485 Phe Asp Ala Leu Leu Lys Leu Val <210> 321 <211> 1134 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1111) <223> FRXA01049 <400> 321 cacagtatgt ggagcagctg cgcgcggaaa tcgatgagaa gcgtctgctt gcacacctcc taccacccat cgcgcttgct ggcctaccac ggccgcaccg gtg gtg aaa act gag ttc gag aaa gag ttc aac Phe G u Lys G u Phe Asn tac gtc tac ttc aaa ctt Tyr Val Tyr Phe Lys Leu gcc gcg tgg agt ggc att Ala Ala Trp Ser Gy Ile aaa gcc aag Lys Ala Lys gca ggc at c Al a G y lle 30 ttg gac gcc Leu Asp Al a 45 Val Val Lys Thr u 1 tat gtg atg acc atc ggt gag Tyr Val IVet Thr Ile Gy u 15 acc gga atg gct act tcg att Thr Gy IVet Ala Thr Ser lie cat acc ggc gaa ctt gat ctg Hi s Thr G y G u Leu Asp Leu Page 407 Gene Sequence 24-2-06.txt act atc ttg gag cac atc ggt gtt gat ccg gct ctg ttc ggt gag atc Leu Phe G y Gu Ile Inr lie Leu uu HMIs lie aga Ar g tgg Tr p tgg Tr p gt c Val ccc Pr o cag G n 150 tgg Tr p at c SIle ggg Gy aac Asn gaa G u 230 gcc Al a gac Asp gt c Val at c SIle ttc Phe 310 cct Pr o cac Hi s t cc Ser gcc Al a 120 cag G n at c SIle gaa G u gaa G u cgc Ar g 200 cag G n ct c Leu gca Al a cca Pr o cct Pr o 280 ctt Leu acg Thr gaa G u gaa G u at t SIle aca Thr ccc Pr o ggt Gy acc Thr 170 ct c Leu at c SIle caa G n ct c Leu cct Pr o 250 ttc Phe gaa G u cag G n cat Hi s cca Pr o 75 gaa G u ggc Gy t cc Ser tct Ser ggc Gy 155 at t SIle gaa G u ggc Gy acc Thr t cc Ser 235 gaa G u ct c Leu at g bet ct c Leu cag G n 315 Sy gcc Al a at c SIle cca Pr o ggc Gy ggc Gy 140 gca Al a at c SIle ggc Gy tgg Tr p ggc Gy 220 t ac Tyr cgg Ar g gcg Al a aag Lys gaa G u 300 ccg Pr o vai Asp r o Ala acc Thr cct Pr o ggc Gy gcc Al a 125 ct g Leu ct c Leu aag Lys acc Thr gca Al a 205 cct Pr o cag G n gt c Val at g bet cgc Ar g 285 cca Pr o cgc Ar g gat Asp tgg Tr p gcc Al a 110 at g Met tgg Tr p aac Asn cct Pr o cca Pr o 190 gcc Al a gaa G u cgc Ar g at c SIle ctt Leu 270 gcc Al a ggc G y ttt Phe gcc Al a ttc Phe 95 gt g Val cgc Ar g tgt Cys gac Asp gaa G u 175 gct Al a t ca Ser cac Hi s gtt Val gca Al a 255 t cc Ser acc Thr ggc G y gcg Al a aaa Lys 80 cat Hi s gat Asp gt g Val t ac Tyr gt c Val 160 aac Asn gt c Val gcg Al a ttg Leu tgg Tr p 240 t ca Ser gac Asp ct c Leu acg Thr cac Hi s 320 gtt Val gcc Al a tct Ser at c SIle cgc Ar g 145 gga Gy ct c Leu ct g Leu cag G n tgg Tr p 225 gaa G u gga G y gcc Al a cgc Ar g cgc Ar g 305 cat Hi s gt c Val at t l Ie aaa Lys ctt Leu 130 gtt Val cgc Ar g gac Asp ccg Pr o gcc Al a 210 cgc Ar g cac Hi s cga Ar g ct c Leu ggc G y 290 gcg Al a t ac Tyr gac Asp cca Pr o acc Thr 115 ccg Pr o t cc Ser gcc Al a gaa G u ttc Phe 195 acg Thr ggc Sy at g Met gt c Val gac Asp 275 acc Thr acg Thr t cc Ser aaa Lys gac Asp 100 gt c Val agc Ser cgc Ar g gt c Val gt g Val 180 ttc Phe at c SIle gtt Val ggg Sy t cc Ser 260 acc Thr gca Al a cca Pr o aag Lys aag Lys ggc G y gca Al a gtt Val gac Asp acc Thr 165 ct g Leu t cc Ser acc Thr ttc Phe aaa Lys 245 acc Thr cca Pr o ctt Leu cca Pr o gca Al a 325 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 Page 408 ene Sequence 24- 2- 06. t xt aga gag ctt ttc gac gcc ctc tac ctc aag ttg gtc tagcttttcg Ar g O31u Leu Phe Asp Al a Leu Tyr Leu Lys Leu Val 330 335 cagt ggaacg cgc <210> 322 <21 1> 337 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1121 1134 <400> 322 ValI vb t vb t G31y Le u ValI Al a Ser I Ie Ar g 145 G31y Le u Le u O31n Tr p 225 O31u G31y Al a Val Lys Thr Thr I Ie (31 2C Al a Thr Ser 03 u Leu Asr Phe G3 y (3 L Val As p LyE I Ie Pr o As r 1 OC Lys Thr Val 115 Leu Pr o Ser 130 Val Ser Ar c Ar g Al a Val As p 03 u Val 1 8C Pr o Phe PhE 1 95 Al a Thr I E 210 Ar g G31y Val Hi s [Vb t (31 Ar g Val Ser 26C Leu Asp Thr 275 O31u Ou le Le u I Ie Lys5 G31y Al a ValI As p Thr 1 65 Le u Ser Thr Ph e Lys5 245 Thr Pr o Phe 03 u Lys 03 u Tyr Al a Thr Ar g 70 Tr p Tr p ValI Pr o O31n 150 Tr p I Ie G31y As n O31u 230 Al a As p ValI ValI Al a I Ie 55 As n Lys5 Pr o Al a O31u 135 cys Le u Ar g O31u I Ie 215 Al a G31y Hi s I Ie Tyr Tr p 40 Le u Pr a Hi s Ser Al a 120 O31n I Ie (31u (31u Ar g 200 O31n Le u Al a Pr a Pr a 280 Ph e 25 Ser O31u As p Le u As n 1 05 Al a I Ie ValI Ar g Pr a 1 85 Ser O31u Al a Al a O31u 265 Le u As n Le u I Ie I Ie Pr a O31u O31y Ser Ser O31y 1 li1e O31u O31y Thr Ser 235 O31u Le u vb t Lys5 Al a Le u O31y Al a I Ie Pr a O31y O31y 140 Al a I Ie O31y Tr p O31y 220 Tyr Ar g Al a Lys5 Al a O31y As p ValI Thr Pr a O31y Al a 125 Le u Le u Ly s Thr Al a 205 Pr a O31n ValI vb t Ar g 285 Ly s I Ie Al a As p As p Tr p Al a 110 vb t Tr p As n Pr a Pr a 1 Al a O31u Ar g I Ie Le u 270 Al a Tyr Thr Hi s Pr a Al a Ph e ValI Ar g cys As p O31u 1 Al a Ser Hi s ValI Al a 255 Se r Th r Arg OyThr Aa Leu ileVal Leu Ou On Leu Ou Prao(y OyThr Page 409 ene Sequence 24- 2- 06. txt 290 295 300 Ar g Ala Thr Pro Pro Phe G31y Thr Thr His On ProArg Phe Ala His 305 310 315 320 Hi s Tyr Ser Lys Ala Ar g G31u Leu Phe Asp Ala Leu Tyr Leu Lys Leu 325 330 335 ValI <210O> 323 <21 1> 597 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 ODS <222> 101). (574) <223> FRXA01050 <400> 323 aagcacagca at tgagcaat act cccat gc at gtt t tcgc aagaat at tc t t tat tagt c agacct t taa aggaaacct t gt gat cacgc t at atcct ta gga t ca at G31y Se r I I ac a Thr at g ac c Thr at c I Ie at c I Ie t ta Le u t ac Tyr at c I Ie t cc Ser 1 t cc Ser at t I Ie cca Pr o gag (31u t ca Ser g at As p t tg Le u g at As p 120 gag (31u c ac Hi s cct Pr o t cc Ser aaa Lys5 gt t ValI at t I Ie at c I Ie g at As p cgt Ar g gcc A a t cg Ser g at As p gca A a ac c Thr ac c Thr ggc G31y gct A a g aa (31u gca A a c ac Hi s 140 tt g Le u g ac As p t ca Ser aag Lys5 45 at t I Ie at t I Ie gt c ValI c ag O31n 125 ggc ct g Le u t ca Ser cga Ar g c ag O31n g at As p t tg Le u gcg A a aat As n 110 t at Tyr cgc Ar g t gg Tr p cgt Ar g 15 ggt G31y cgc Ar g gct A a aac As n ct a Le u 95 gcg A a gt g ValI ac c Thr gga G31y gga G31y g aa (31u g ac As p gcg A a 80 g at As p ct c Le u gag (31u ggc G31y t at Tyr t at Tyr cat Hi s gt g ValI g at As p tt t Ph e ccg Pr o ct g Le u 130 t gc ct Le 2 gc A t t Ph t c Se ca Hi t c Se 10 at I I gc A ca t cca e Pr o t gct u A a 0 t t cc a Ser c acc e Thr g gag r (1u c aac s As n c t ca r Ser 0 t acc e Thr g gaa a (1u c acc Cys Leu Hi s Thr t gaaaact ga gt t cgagaaa Page 410 Gene Sequence 24-2-06.txt gag 597 <210> 324 <211> 158 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 324 eVt G y Ser 1 Pr o Leu Ser G n Al a Ser Thr G n Val 145 Val Asp G u Val Hi s Al a Cys 115 Ar g Leu II e Pro Thr 5 Leu Al a IVet Al a Ser G y Phe Thr Thr Ser G u Il e 70 His Asn Il e Ser Ser Leu 100 I e Thr Tyr Ala G u Il e Hi s Thr Ser Asp Cys Gy Thr Lys SIle Al a Asp 135 Tyr Gy 25 I le Gy Val G n Val 105 Ser Lys Pr o 10 Ser Lys Val SIle I e 90 Asp Ar g Al a Ser Thr Gy Ser Asn Al a Gy Ser Tyr Ar g 155 IVet Ser Ile Pro Phe Asp Asp Ser Al a Thr Thr Gy Al a G u Al a Hi s 140 Leu Ar g G n Asp Leu Al a Asn 110 Tyr Ar g Tr p Ar g Gy Ar g Al a Asn Leu Al a Val Thr <210> 325 <211> 1065 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1042) <223> RXA00202 <400> 325 ctggcagcag attgtcatcg gttgtgtcat cgcgcttgcg gtgggcttcg atgtcatccg aaacaaaacc tctaagtaat tcctgaaagg aaattttcac atg tac gct cgt aaa IVet Tyr Ala Arg Lys 1 ctt att gct ctg tcc gct tct gtc gtt ttg gct ttc agc ttg tct gct Leu lie Ala Leu Ser Ala Ser Val Val Leu Ala Phe Ser Leu Ser Ala 15 tgc aac cgt gaa tct tct ggc acc agc gca gac ggc ggt tct gcg gat Cys Asn Arg u Ser Ser y Thr Ser Ala Asp Gy y Ser Ala Asp 30 ggg tcg atc acc ttg gct ctg tct acc cag acc aac ccg ttc ttt gtg Sy Ser lie Thr Leu Al a Leu Ser Thr G n Thr Asn Pro Phe Phe Val Page 411 Gene Sequence 24-2-06.txt cag G n ct c Leu ct c Leu act Thr gac Asp gcg Al a gca Al a 150 caa G n gaa G u acc Thr ct g Leu at g et 230 gt c Val gaa G u gga Gy gct Al a cgt Ar g gtt Val aac Asn tct Ser cct Pr o 120 ttc Phe ct g Leu at t SIle gag G u aac Asn 200 gca Al a ttg Leu gtt Val gga Gy aag Lys 280 aca Thr ggt Gy gat Asp gt c Val gct Al a gt g Val gca Al a gag G u gga Gy 170 gct Al a gac Asp ccc Pr o gca Al a ggt Gy 250 at g let gt g Val gt a Val gcc Al a gct Al a 75 acc Thr gt g Val gct Al a tct Ser gcg Al a 155 t cc Ser aag Lys cgc Ar g aat Asn at c l Ie 235 tt c Phe tt g Leu gaa G u cca Pr o cag G n t cc Ser acc Thr gt g Val gt c Val gac Asp 140 at c l Ie tct Ser cat Hi s ggt Gy gt g Val 220 gaa G u gat Asp gcc Al a gaa G u gtt Val 300 gaa G u gat Asp ggt Gy ccg Pr o gac Asp 125 aac Asn ggt Gy gca Al a gag G u gag G u 205 aag Lys gcc Al a ggc Gy acc Thr gca Al a 285 gag G u aag Lys gac Asp gct Al a tcg Ser 110 cgt Ar g gtt Val ggc Gy t ca Ser ggc Gy 190 ggc Gy gcg Al a ct g Leu acc Thr gtt Val 270 gct Al a gtt Val gct Al a gct Al a ggc Gy 95 gt g Val t cc Ser gct Al a gaa G u cgt Ar g 175 at t SIle ct g Leu at c l Ie ggt Gy aat Asn 255 gct Al a aag Lys gt c Val gat gaa Asp u gca acg Al a Thr gtg gt g Val Val gaa gct G u Al a t cc aat Ser Asn ggc ggc G y G y 145 ggt gaa G y G u 160 gat cgt Asp Arg tcc att Ser Ile gac gtg Asp Val ttc gcg Phe Al a 225 gct cgt Al a Ar g 240 gat ggt Asp y cag cag G n n ct c ct g Leu Leu act gtg Thr Val 305 ttg Leu cag G n at t SIle ct c Leu ggt Sy 130 gcg Al a at c SIle gga G y gt g Val gca Al a 210 gaa G u gct Al a ct g Leu cca Pr o cgc Ar g 290 aag Lys ggc G y gcc Al a gt c Val aac Asn 115 ggc Gy cag G n ct c Leu cag G n gct Al a 195 act Thr aac Asn ggt Sy gca Al a gaa G u 275 ggt Sy ct c Leu gt g Val aac Asn aac Asn 100 cag G n gag G u gct Al a at g let gga G y 180 aag Lys aac Asn gat Asp gaa G u gcg Al a 260 gag G u gag G u gac Asp 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1065 gtc gcg gac ttc aag Val Ala Asp Phe Lys t agt cggcga t gaaaaagt c cgt Page 412 Gene Sequence 24-2-06.txt 310 <210> 326 <211> 314 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 326 IVet Tyr Ala Arg Lys Leu Ile Ala Leu Ser Ala Ser Val Val Leu Ala 1 5 10 Phe Ser Leu Ser Ala Cys Asn Arg u Ser Ser y Thr Ser Ala Asp 25 Gy y Ser Ala Asp G y Ser lie Thr Leu Ala Leu Ser Thr G n Thr 40 Asn Pro Phe Phe Val G n Leu Arg Asp y Ala n G u Lys Ala Asp 55 G u Leu y Val Thr Leu Asn Val G n Asp Ala Ser Asp Asp Ala Ala 70 75 Thr G n Ala Asn G n Leu Asn Asn Ala Val Thr Thr y Al a y Val 90 Val lie Val Asn Pro Thr Asp Ser Asp Ala Val Val Pro Ser Val G u 100 105 110 Ala Leu Asn G n Ala Asp lie Pro Val Val Ala Val Asp Arg Ser Ser 115 120 125 Asn Gy Gy G u Val Al a Ser Phe Val Ala Ser Asp Asn Val Ala y 130 135 140 Sy Ala n Ala Ala Ala Ala Leu Ala u Ala lle y y u y 145 150 155 160 Su Ile Leu IVet Leu n y Ile Ala y Ser Ser Ala Ser Arg Asp 165 170 175 Arg G y n G y Phe G u G u G u IIe Al a Lys His G u G y IIe Ser 180 185 190 Ile Val Ala Lys n Thr Ala Asn Phe Asp Arg Gy Gu y Leu Asp 195 200 205 Val Ala Thr Asn Leu Leu G n Ala His Pro Asn Val Lys Ala lie Phe 210 215 220 Al a u Asn Asp G u IVet Al a Leu y Ala lie u Ala Leu G y Al a 225 230 235 240 Ar g Al a G y u Asp Val ile Val Val G y Phe Asp G y Thr Asn Asp 245 250 255 G y Leu Al a Al a Val G u Asp G y Arg et Leu Al a Thr Val Al a G n 260 265 270 Sn Pro u u Leu G y Ala Lys Ala Val G u G u Ala Ala Lys Leu 275 280 285 Leu Arg G y G u Asp Al a G u Thr G u Val Pro Val G u Val Val Thr 290 295 300 Val Lys Leu Asp Asn Val Ala Asp Phe Lys 305 310 Page 413 ene Sequence 24- 2- 06. txt <210O> 327 <21 1> 1077 <212> DNA <213> COr ynebact er i urn gi ut arr cumn <220> <221 ODS <222> (1054) <223> RXN00872 <400> 327 gaaat gtat t gct t tgt cag gacaat gt gt t at tgt cat g ccacat tcca t caaaaat ga gt gaagggt t gcat cgccac acat gcgat c gt gagggt cg atg act aac ttg acg 11! l'bt Thr Asn Leu Thr ag c Ser cca Pr o t ac Tyr gga G31y gag O31u gt t ValI tt c Ph e ccg Pr o g aa O31u ag c Ser 1 50 c ac Hi s g aa O31u c ac Hi s c aa O31n G31y aat As n ct g Le u ac c Thr ccg Pr o ct c Le u 120 g at As p ggc G31y at c I Ie gcc A a gt c ValI gga G31y ag c Ser gca A a gct A a c ag O31n g at As p at t I Ie tt g Le u c ac Hi s g at As p 1 70 aag Ly s ct a Le u gt a ValI gca A a ct g Le u gag O31u 75 act Thr t t c Ph e gaa O31u t gg Tr p cgc Ar g 155 ct g Le u cag O31n gct A a gga G31y gca A a ct g Le u ct g Le u tt t Ph e cca Pr a t cc Ser tt c Ph e 140 gag (31u g ac As p gcg A a at c I Ie ct g Le u aac As n 45 t cc Ser gag (31u aag Ly s ct g Le u gca A a 125 ac a Thr at c I Ie t ac Tyr g aa (31u ggc G31y gcc A a gt t ValI cgt Ar g cgt Ar g ac c Thr t ac Tyr 110 g ac As p ct c Le u tt g Le u cga Ar g t gg Tr p 1 cgc Ar g 15 g at As p t ct Ser gt g ValI tt g Le u cca Pr a 95 tt c Ph e gt c ValI act Thr act Thr cca Pr a 1 75 gcg A a tt g Le u gt t ValI gt t ValI gga G31y ggc G31y gt g ValI t ac Tyr ag c Ser ggt G31y act Thr 1 at g vb t tt g Le u 1 ggc G31y c aa O31n gca A a aat As n gt g ValI ac c Thr cgc Ar g ct g Le u tt c Ph e 145 cgt Ar g tt c Ph e c ag O31n gt a ValI t ct Ser gcc A a g at As p g ac As p tt c Ph e g aa O31u g ac As p 130 agt Ser gcg A a t gg Tr p cat Hi s at t lIe ggc O31y cgc Ar g tt c Ph e c ag O31n g aa O31u 1 00 aag Ly s gt g ValI gag O31u cgt Ar g t cc Se r 1 ac g Th r gcg gtt ggc aac aag gaa gaa tgc gaa atc gca gtg ggc gag acc gag Page 414 Ala Val Gy Asn Lys Gu Gu 200 cca gag cgc gcg ggc cga gca Pro G u Arg Ala Gy Arg Al a 215 220 atc gtc aag cag gga cct aag lie Val Lys G n y Pro Lys 230 235 acc gta gaa gtt cct ccg ttc Thr Val G u Val Pro Pro Phe 250 gcc ggc gat gca ttc ggc ggc Al a y Asp Al a Phe G y G y 265 tgg ccg ttg gaa aag gtt ctc Trp Pro Leu G u Lys Val Leu 280 gtg gcg tcc cgt ctt gaa tgc Val Ala Ser Arg Leu G u Cys 295 300 gtg gaa gcc tcc ctc aac cag Val G u Ala Ser Leu Asn G n 310 315 t ct <210> 328 <211> 318 <212> PRT <213> Cor ynebact er i um gl ut ar Gene Sequence 24- 2- 06. txt Cys Gu Ile Ala Val Gy Gu Thr 205 210 ctg ttg gaa cgc ggt gtg gag ttg Leu Leu G u Arg Gy Val G u Leu 225 ggt gtc atg gcg atg acc aag gac G y Val IVet Al a IVet Thr Lys Asp 240 ttc gtc gat gtc atc aac ggt ctt Phe Val Asp Val lie Asn Gy Leu 255 260 gcg ctg tgc cac ggt ctg ctc tct Al a Leu Cys Hi s G y Leu Leu Ser 270 275 cgt ttt gcc aac acc gcg ggt gcg Arg Phe Ala Asn Thr Ala Gy Ala 285 290 t cc acc gca atg cct act acc gat Ser Thr Ala IVet Pro Thr Thr Asp 305 aaa gtc tgatatgact cctccgatta Lys Val 787 835 883 931 979 1027 1074 1077 <400> 328 IVet Thr Asn Leu Thr Ser Thr His Ou Val Leu Ala Ile Gy 1 Gy G n Al a Asn Val Thr Ar g Leu Phe Asp Phe Al a Pr o Asn Cys Pr o 115 Asp G u 5 Tyr Lys Hi s Gy Tyr I le Al a Ar g Pr o Leu Leu Hi s Tyr Al a Pr o Asp Al a 135 Ar g Ser Gy Ser Leu Asp Asp 105 Asn l Ie Thr 10 Sy Val y Ser Al a Al a Ala Leu Leu Al a G u Leu G n Thr Phe 90 Asp Phe Pro Ile u Ser Leu Trp Phe 140 His Arg G u Page 415 Leu Asn Ser G u Lys Leu Al a 125 Thr SIle Al a Val Ar g Ar g Thr Tyr 110 Asp Leu Leu Ar g Asp Ser Val Leu Pr o Phe Val Thr Thr Gene Sequence 24-2-06.txt 155 Arg Ala Asn Arg Arg His Thr Ile Phe Asp Leu 165 170 Phe Trp G u Ser Pro G u G u Al a Thr Lys G n 180 185 G n His Ser Thr Val Al a Val G y Asn Lys G u 195 200 Val G y G u Thr G u Pro G u Arg Ala G y Arg 210 215 Sy Val u Leu Ala I e Val Lys n y Pro 225 230 235 IVet Thr Lys Asp G u Thr Val G u Val Pro Pro 245 250 I e Asn G y Leu G y Al a y Asp Al a Phe G y 260 265 Sy Leu Leu Ser G u Trp Pro Leu G u Lys Val 275 280 Thr Ala y Ala Leu Val Ala Ser Arg Leu G u 290 295 Pro Thr Thr Asp G u Val G u Ala Ser Leu Asn 305 310 315 <210> 329 <211> 622 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(622) <223> FRXA00872 <400> 329 gaaatgtatt gctttgtcag gacaatgtgt tattgtcatg ccacattcca tcaaaaatga gtgaagggtt gcatcgccac Asp Tyr Ar g Al a G u Al a 220 Lys Phe Gy Leu Cys 300 G n u Cys 205 Leu Gy Phe Al a Ar g 285 Ser Lys Pr o 175 Al a SIle G u et Asp 255 Cys Al a Al a bet Leu Al a Ar g Al a 240 Val Hi s Asn bet agc Ser cca Pr o t ac Tyr gga Gy gag G u cac Hi s caa G n ggc Gy aat Asn ct g Leu gt c Val gga Gy agc Ser gca Al a gct Al a ct a Leu gt a Val gca Al a ct g Leu gag G u gct Al a gga Gy gca Al a ct g Leu ct g Leu ggc Gy gcc Al a gtt Val cgt Ar g cgt Ar g cgc ttg Arg Leu 15 gat gtt Asp Val tct gtt Ser Val gtg gga Val G y ttg ggc Leu G y Page 41E acatgcgatc gtgagggtcg atg act aac ttg acg IVet Thr Asn Leu Thr 1 ggc gta gat att tac Sy Val Asp Ile Tyr caa tct ttc ggc aag G n Ser Phe y Lys gca gcc gcc cgc cat Ala Ala Ala Arg His aat gat cct ttc ggc Asn Asp Pro Phe y gtg gac aac cag tac Val Asp Asn G n Tyr gtt Val ttc Phe ccg Pr o gaa G u agc Ser 150 cac Hi s acc Thr ccg Pr o ct c Leu 120 gat Asp ggc Gy at c SIle cag G n gat Asp at t SIle ttg Leu cac Hi s gat Asp 170 ttt Phe cca Pr o t cc Ser ttc Phe 140 gag G u gac Asp Gene Sequence 24-2-06.txt 80 aag acc cca gtg acc ttc tgt Lys Thr Pro Val Thr Phe Cys 95 ctg tac ttc tac cgc gaa cca Leu Tyr Phe Tyr Arg G u Pro 110 115 gca gac gtc agc ctg gac gat Ala Asp Val Ser Leu Asp Asp 125 130 aca ctc act ggt ttc agt gaa Thr Leu Thr y Phe Ser G u 145 atc ttg act act cgt gcg aac Ile Leu Thr Thr Arg Ala Asn 160 tac cga Tyr Arg gaa G u 100 aag Lys gt g Val gag G u cgt Ar g att SIe gct Al a cgc Ar g cca Pr o cgc Ar g 165 <210> 330 <211> 174 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 330 IVet Thr Asn Leu Thr Ser Thr His G u 1 Gy G n Al a Asn Val Thr Ar g Leu Phe 145 Ar g Asp Phe Al a Pr o Asn Cys Pr o 115 Asp G u Asn 5 Tyr Lys Hi s Gy Tyr I le Al a Ar g Pr o Ar g 165 Leu Leu Hi s Tyr Al a Pr o Asp Al a 135 Ar g Thr Ser Gy Ser Leu Asp Asp 105 Asn l Ie Thr Phe Val 10 Gy Ser Al a Al a G n 90 Asp SIle Leu Hi s Asp 170 Leu Val Al a Leu G u Thr Phe G u Tr p Ar g 155 Leu Al a Gy Al a Leu Leu Phe Pr o Ser Phe 140 G u Asp l Ie Leu Asn Ser G u Lys Leu Al a 125 Thr SIle Tyr Gy Al a Val Ar g Ar g Thr Tyr 110 Asp Leu Leu Ar g Ar g Asp Ser Val Leu Pr o Phe Val Thr Thr <210> 331 Page 417 Gene Sequence 24-2-06.txt <211> 1767 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1744) <223> RXN00799 <400> 331 ttgtctaacc ccgtataggt gagaaatgtt ggacaagtgt ctgttttttg tgggggaaat ctgactacga tggtaagaaa taaggaaaga gattaccatt cct Pr o caa G n tgg Tr p aat Asn gga Sy tgc Cys at c SIle gaa G u aac Asn gt c Val 150 cct Pr o gcg Al a cgc Ar g at c SIle cgc Ar g ct a Leu gaa G u tcg Ser cct Pr o ggt Sy gcc Al a 120 cca Pr o gag G u aac Asn gt g Val gct Al a 200 t cc Ser ct c Leu cca Pr o aaa Lys ttg Leu ct g Leu cct Pr o aac Asn cgt Ar g t cc Ser gt c Val 170 tct Ser ct g Leu cgc Ar g gac Asp gca Al a gcg Al a gcg Al a 75 aac Asn tt c Phe ct g Leu ggg G y cgt Ar g 155 gcg Al a gag G u at g bet ct c Leu ggc G y gag Gu ggc Sy ccg Pr o cct Pr o acc Thr cgc Ar g ctt Leu 140 ttg Leu ct c Leu tgg Tr p gag G u cgt Ar g ggc G y gcc Al a at g et agt Ser gat Asp cct Pr o 110 cag G n act Thr at t SIle gcg Al a ggc Gy 190 ttc Phe gt c Val 15 gt a Val gct Al a at c SIle gaa G u at g bet 95 gt g Val cgt Ar g tgg Tr p cct Pr o cag G n 175 gag gtu ggt Gy at t SIle ctt Leu gac Asp at c SIle ggc Gy 80 tgg Tr p ct g Leu t ac Tyr acc Thr gtt Val 160 ttc Phe ct g Leu acc Thr at g bet 1 gaa G u gca Al a ct g Leu ggt Gy aaa Lys cgt Ar g gca Al a tta Leu aat Asn 145 gcg Al a ggt Gy ggt ggy gag G u tct caa gag cgg Ser G n G u Arg caa gac ggc cta G n Asp G y Leu cct tat gaa gat Pro Tyr G u Asp gtg aaa cgt atg Val Lys Arg IVet tcg cac tac ccc Ser His Tyr Pro gac gcg gaa aag Asp Ala G u Lys gag gat aac ccg G u Asp Asn Pro 100 act tct tcc act Thr Ser Ser Thr 115 att gtt cgt gac Ile Val Arg Asp 130 gct gtt cag gaa Ala Val n u ttt gcg tcg aat Phe Ala Ser Asn 165 gtg aac gag tcc Val Asn u Ser 180 ctt gct gcg ctt Leu Ala Ala Leu 195 gct gct aaa gaa Ala Ala Lys G u 210 Page 418 Gene Sequence 24-2-06.txt tgg Tr p gct Al a 230 ccg Pr o ggc Gy cca Pr o ggt Gy ct g Leu 310 cct Pr o cag G n t ac Tyr cgt Ar g ggc Sy 390 gca Al a gaa G u gt c Val cca Pr o gtt Val 470 gcc ggt ggt gt g Al a y y Val cac aag ctg tac His Lys Leu Tyr gag G u ttg Leu gag G u ggc Sy 280 acc Thr cct Pr o t ac Tyr tgg Tr p cgc Ar g 360 t ac Tyr gag G u acc Thr gtt Val cga Ar g 440 gt c Val gca Al a cgt Ar g tct Ser 250 t cc Ser gt g Val gag G u cag G n cgg Ar g 330 aac Asn ttc Phe aac Asn ct c Leu at t SIle 410 gag G u ct g Leu gaa G u ggc Gy tgg Tr p 235 gat Asp aag Lys cgc Ar g tac Tyr gca Al a 315 cca Pr o ccg Pr o at t SIle t cc Ser agc Ser 395 tt c Phe gga G y gag G u gat Asp aac Asn 475 220 t cc Ser t ac Tyr aat Asn ct c Leu cca Pr o 300 gct Al a at g let acc Thr cag G n gac Asp 380 gag G u t cc Ser cac Hi s gaa G u gaa G u 460 aaa Lys cgc Ar g at c SIle t cc Ser gac Asp 285 acc Thr at c l Ie aac Asn acg Thr gat Asp 365 t cc Ser cca Pr o gac Asp ctt Leu at c SIle 445 gca Al a gca Al a ttc Phe gct Al a gt g Val 270 ggc G y gaa G u gac Asp aac Asn cag G n 350 ttg Leu ggc Gy gaa G u at g let gat Asp 430 ttc Phe gaa G u cag G n aac Asn gct Al a 255 tcg Ser cac Hi s gat Asp gct Al a t cc Ser 335 ttc Phe ctt Leu gt c Val cgc Ar g gct Al a 415 gag G u cag G n aag Lys ctt Leu ggt Sy ggt Gy 240 gtt Val acc Thr gat Asp gcg Al a ggc Gy 320 gcc Al a gaa G u cgc Ar g at c SIle ttc Phe 400 aac Asn t ca Ser ctt Leu at c SIle gat Asp 480 t ac Tyr 225 act Thr gt g Val acc Thr cct Pr o ct g Leu 305 tgc Cys aac Asn gag G u gac Asp gac Asp 385 gcc Al a cca Pr o gag G u ggt G y at t SIle 465 t cc Ser ttt Phe cgt Ar g at t SIle cac Hi s 290 ggc G y gcc Al a cag G n gtt Val gcc Al a 370 gcc Al a gca Al a cgt Ar g ct g Leu ct g Leu 450 ggt G y gt c Val ggt Gy ggt G y aag Lys 275 ttc Phe aag Lys tcg Ser ct c Leu gcg Al a 355 at g et at g et gca Al a cga Ar g aat Asn 435 ttt Phe gcg Al a acc Thr atg gct gac ctc IVet Al a Asp Leu gag G u ttg Leu 260 cac Hi s cac Hi s t ac Tyr at c SIle gat Asp 340 ttt Phe ggc Sy at g Net gt g Val ct g Leu 420 cag G n gag G u cca Pr o ttg Leu gat Asp 245 cag G n ttc Phe tgg Tr p cat Hi s at g bet 325 cag G n gcc Al a cac Hi s tgg Tr p cgt Ar g 405 ct c Leu cca Pr o aac Asn gag G u ct g Leu 485 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 Page 419 cgt aac aac ccc atc cgt gct Arg Asn Asn Pro lie Arg Ala 490 gat cta ccc att ggt tac tgg Asp Leu Pro lie y Tyr Trp 505 aca gct gga agc agc cat tcg Thr Ala G y Ser Ser His Ser 520 gtc ttc cga gtc aga agc aga Val Phe Arg Val Arg Ser Arg 535 540 tgaaattgca ctgtttgaag at g <210> 332 <211> 548 <212> PRT <213> Cor ynebact er i um gl ut ar Gene Sequence 24-2-06.txt gcc act gga tcc tgc agc aag cct Ala Thr y Ser Cys Ser Lys Pro 495 500 ccg tac caa gat cga cga ggt tca Pro Tyr G n Asp Arg Arg y Ser 510 515 cgc aga act ccc agg ggt aac ctt Arg Arg Thr Pro Arg y Asn Leu 525 530 tct tgc aat cgt gtg ggc tcg ccc Ser Cys Asn Arg Val y Ser Pro 545 1603 1651 1699 1744 1767 <400> 332 Mvt Ser G 1 Gu GnA Al a Pro T Leu Val L Sy Ser H Lys Asp A Arg Gu A Al a Thr S 1 Leu I e V 130 Asn Al a V 145 Al a Phe A Sy Val A G y Leu A 1 G u Al a A 210 In Gu sp Gy yr G u ys Arg is Tyr I a u sp Asn 100 er Ser 15 al Ar g al n I a Ser sn G u 180 I a Al a 95 I a Lys Ar g 5 Leu Asp bet Pr o Lys Pr o Thr Asp G u Asn 165 Ser Leu G u Pro Gn lie G y G n Tr p Asn Gy 70 Cys SIle G u Asn Val 150 Pr o Al a Ar g Tr p Phe Ar g Val Tyr G u Thr Asn Leu 135 Al a Ar g Gy Asp Ar g 215 Ar g Leu 40 G u Ser Pr o Gy Al a 120 Pr o G u Asn Val Al a 200 Al a Asp 25 Thr G u Pr o Leu Val 105 l le Al a Ar g Hi s Phe 185 G u Gy Ar g Asp Al a Al a Al a Asn Phe Leu Gy Ar g 155 Al a G u Met Val Ser Asp Ar g Leu G u Val G u Asn 125 Al a Gy Val Pr o Thr 205 Lys Ar g Gy Al a et Ser Asp Pr o 110 G n Thr SIle Al a Gy 190 Phe Leu Val Val Al a SIe G u vet Val Ar g Tr p Pr o G n 175 G u Gy Tyr Tyr IVet Ala Asp Leu Ala Ser u Pro Arg Trp Ser Arg Phe Asn y Page 420 Gene Sequence 24-2-06. txt 225 Thr Val Thr Pr o Leu 305 Cys Asn G u Asp Asp 385 Al a Pr o G u Gy SIle 465 Ser Cys Ar g Ar g Val 545 Gy Gy Lys 275 Phe Lys Ser Leu Al a 355 bet et Al a Ar g Asn 435 Phe Al a Thr Lys Gy 515 Asn Ser Asp 245 G n Phe Tr p Hi s bet 325 G n Al a Hi s Tr p Ar g 405 Leu Pr o Asn G u Leu 485 G u Thr Gy G u Pr o Gy G n 295 Pr o Tyr Leu Asn Gy 375 Val Gy Al a G n Tyr 455 Ser Asn Leu Al a Phe 535 Leu II e Ser 250 G u Leu Ser 265 G y G y Val 280 Thr Asn G u Pro Phe G n Tyr Al a Arg 330 Trp n Asn 345 Arg Thr Phe 360 Tyr Val Asn G u G u Leu Thr Asp II e 410 Val Ala u 425 Arg Leu Leu 440 Val Ser G u Al a Leu G y Asn Pro Ile 490 Pro lie G y 505 G y Ser Ser 520 Arg Val Arg 235 Asp Tyr Ile Ala Ala 255 Lys Asn Ser Val Ser 270 Arg Leu Asp G y His 285 Tyr Pro Thr G u Asp 300 Ala Ala l e Asp Ala 315 Pro IVet Asn Asn Ser 335 Pro Thr Thr G n Phe 350 Ile G n Asp Leu Leu 365 Ser Asp Ser G y Val 380 Ser G u Pro G u Arg 395 Phe Ser Asp IVet Ala 415 G y Hi s Leu Asp G u 430 G u G u lie Phe G n 445 Asp G u Al a u Lys 460 Asn Lys Ala G n Leu 475 Arg Ala Ala Thr y 495 Tyr Trp Pro Tyr G n 510 His Ser Arg Arg Thr 525 Ser Arg Ser Cys Asn 540 <210> 333 <211> 1607 <212> DNA <213> Corynebacter i um gl utam cum Page 421 Gene Sequence 24-2-06.txt <220> <221> CDS <222> (1)..(1584) <223> FRXA00799 <400> 333 ct a Leu 1 gat Asp at g bet ccc Pr o aag Lys ccg Pr o act Thr gac Asp gaa G u aat Asn 145 t cc Ser ctt Leu gaa G u ct c Leu gat Asp 225 cag G n ttc Phe cgt Ar g gt g Val t ac Tyr gag G u acg Thr aat Asn ct g Leu 115 gcg Al a cgt Ar g ggt Gy gat Asp cgt Ar g 195 tct Ser gag G u cct Pr o gat Asp 5 acc Thr gaa G u cct Pr o ttg Leu gtt Val at t SIle gct Al a cga Ar g cac Hi s ttc Phe 165 gaa G u ggt Gy cct Pr o at c SIle ct g Leu ct c Leu cca Pr o aaa Lys tt g Leu ct g Leu 70 cct Pr o aac Asn cgt Ar g t cc Ser gt c Val 150 tct Ser ct g Leu ggt Gy cgt Ar g tct Ser 230 t cc Ser gac Asp gca Al a gcg Al a gcg Al a aac Asn ttc Phe ct g Leu ggg Gy cgt Ar g 135 gcg Al a gag G u at g et gt g Val tgg Tr p 215 gat Asp aag Lys gac ggc gta Asp G y Val 10 cgt gcc gct Ar g Al a Al a ct g at g at c Leu IVet I e gag agt gaa Su Ser G u gtc gat at g Val Asp Vbet 75 gag cct gtg Su Pro Val 90 aat cag cgt Asn n Arg 105 gct act tgg Al a Thr Trp ggt att cct Gy lle Pro gtt gcg cag Val Ala n 155 cct ggc gag Pro G y G u 170 act ttc ggt Thr Phe G y 185 aag ct g t ac Lys Leu Tyr cgc ttc aac Arg Phe Asn at c gct gct I e Al a Al a 235 tcc gtg tcg Ser Val Ser gca Al a ct g Leu ggt Gy aaa Lys cgt Ar g gca Al a tta Leu aat Asn 125 gcg Al a ggt Gy ggt Gy gag G u t ac Tyr 205 act Thr gt g Val cct Pr o gt g Val tcg Ser gac Asp gag G u act Thr at t SIle 110 gct Al a ttt Phe gt g Val ctt Leu gct Al a 190 at g et tt t ttt Phe cgt Ar g t at Tyr aaa Lys cac Hi s gcg Al a gat Asp tct Ser gtt Val gtt Val gcg Al a aac Asn gct Al a 175 gct Al a gct Al a ggt Gy ggt Gy gaa G u cgt Ar g t ac Tyr gaa G u aac Asn t cc Ser cgt Ar g cag G n tcg Ser gag G u 160 gcg Al a aaa Lys gac Asp gag G u ttg Leu 240 acc acc att Thr Thr Il e aag cac Lys Hi s Page 422 Gene Sequence 24-2-06. t xt 245 250 255 ttc cca ggt ggc ggc gtg cgc ctc gac ggc cac gat cct cac ttc cac 816 Phe Pro 3 y 3 y 3 y Val Arg Leu Asp 3 y His Asp Pro His Phe His 260 265 270 tgg ggt cag acc aat gag tac cca acc gaa gat gcg ctg ggc aag tac 864 Trp y n Thr Asn u Tyr Pro Thr u Asp Ala Leu y Lys Tyr 275 280 285 cat ctg cct cct ttc cag gca gct atc gac gct ggc tgc gcc tcg atc 912 His Leu Pro Pro Phe O n Ala Ala lie Asp Ala O y Cys Ala Ser Ile 290 295 300 atg cct tac tac gca cgg cca atg aac aac tcc gcc aac cag ctc gat 960 'b t Pro Tyr Tyr Ala Arg Pro 'b t Asn Asn Ser Ala Asn n Leu Asp 305 310 315 320 cag cag ctg tgg caa aac ccg acc acg cag ttc gaa gag gtt gcg ttt 1008 n O n Leu Trp n Asn Pro Thr Thr n Phe u u Val Ala Phe 325 330 335 gcc tac aac cgc acc ttc att cag gat ttg ctt cgc gac gcc atg ggc 1056 Ala Tyr Asn Arg Thr Phe Ile n Asp Leu Leu Arg Asp Ala 'b t y 340 345 350 cac cgt ggg tac gtc aac tcc gac tcc ggc gtc atc gac gcc atg atg 1104 His Arg O y Tyr Val Asn Ser Asp Ser O y Val Ile Asp Ala 'b t 'b t 355 360 365 tgg ggc gtg gag gaa ctc agc gag cca gaa cgc ttc gcc gca gca gtg 1152 Trp y Val u u Leu Ser u Pro u Arg Phe Ala Ala Ala Val 370 375 380 cgt gca ggc acc gac att ttc tcc gac atg gct aac cca cgt cga ctg 1200 Arg Ala y Thr Asp Ile Phe Ser Asp 'b t Ala Asn Pro Arg Arg Leu 385 390 395 400 ctc gaa gca gtt gct gag gga cac ctt gat gag t ca gag ctg aat cag 1248 Leu O u Ala Val Ala O u O y His Leu Asp u Ser O u Leu Asn O n 405 410 415 cca gtc cag cga ctc ctg gag gaa atc ttc cag ctt ggt ctg ttt gag 1296 Pro Val n Arg Leu Leu u u Ile Phe n Leu y Leu Phe u 420 425 430 aac cca tat gtc tct gaa gat gaa gca gaa aag atc att ggt gcg cca 1344 Asn Pro Tyr Val Ser u Asp u Ala u Lys Ile Ile y Ala Pro 435 440 445 gag gtt tct gca ttg ggc aac aaa gca cag ctt gat tcc gtc acc ttg 1392 Su Val Ser Ala Leu y Asn Lys Ala n Leu Asp Ser Val Thr Leu 450 455 460 ctg cgt aac aac ccc atc cgt gct gcc act gga tcc tgc agc aag cct 1440 Leu Arg Asn Asn Pro Ile Arg Ala Ala Thr y Ser Cys Ser Lys Pro 465 470 475 480 gaa gat cta ccc att ggt tac tgg ccg tac caa gat cga cga ggt tca 1488 Su Asp Leu Pro Ile y Tyr Trp Pro Tyr n Asp Arg Arg y Ser 485 490 495 act aca gct gga agc agc cat tcg cgc aga act ccc agg ggt aac ctt 1536 Thr Thr Ala O y Ser Ser His Ser Arg Arg Thr Pro Arg O y Asn Leu 500 505 510 ggt gtc ttc cga gtc aga agc aga tct tgc aat cgt gtg ggc tcg ccc 1584 Sy Val Phe Arg Val Arg Ser Arg Ser Cys Asn Arg Val y Ser Pro Page 423 Gene Sequence 24-2-06.txt 515 520 525 tgaaattgca ctgtttgaag at g <210> 334 <211> 528 <212> PRT <213> Corynebacter i um gl ut ai cum 1607 <400> 334 Leu G n Phe 1 Asp bet Pr o Lys Pr o Thr Asp G u Asn 145 Ser Leu G u Leu Asp 225 G n Phe Tr p Hi s Ar g Val Tyr G u Thr Asn Leu 115 Al a Ar g Gy Asp Ar g 195 Ser G u Pr o Gy G n 275 Pr o Arg Asp Leu 5 Leu Thr Pro G u G u Lys Ser Pro Leu Pro Leu Leu 70 G y Val Pr o Al a I e Asn 100 Pro Al a Ar g G u Arg Ser Asn Hi s Val 150 Val Phe Ser 165 Ala G u Leu 180 Al a G y G y G u Pro Ar g Leu Ile Ser 230 G u Leu Ser 245 G y G y Val 260 Thr Asn G u Pro Phe G n Asp Al a Al a Al a Asn Phe Leu Gy Ar g 135 Al a G u et Val Tr p 215 Asp Lys Ar g Tyr Al a 295 Asp Ar g Leu G u Val G u Asn 105 Al a Gy Val Pr o Thr 185 Lys Ar g SIle Ser Asp 265 Thr l Ie G y Val Leu 10 Al a Al a Asp IVet iIe Ile Ser G u G y Asp IVet Trp 75 Pro Val Leu 90 G n Arg Tyr Thr Trp Thr li e Pro Val 140 Al a G n Phe 155 G y G u Leu 170 Phe G y Thr Leu Tyr G y Phe Asn G y 220 Al a Al a Val 235 Val Ser Thr 250 G y Hi s Asp G u Asp Al a Asp Al a G y 300 Al a Leu Gy Lys Ar g Al a Leu Asn 125 Al a Gy Gy G u Tyr 205 Thr Val Thr Pr o Leu 285 Cys Tyr Lys Hi s Al a Asp Ser Val Val Al a Asn Al a 175 Al a Al a Gy Gy Lys 255 Phe Lys Ser G u Ar g Tyr G u Asn Ser Ar g G n Ser G u 160 Al a Lys Asp G u Leu 240 Hi s Hi s Tyr SIe Page 424 Gene Sequence 24- 2- 06. txt Pro IVet Asn Asn Ser Ala Asn n Leu lIet Pro Tyr Tyr Ala Arg 305 310 G n Al a Hi s Tr p Ar g 385 Leu Pr o Asn G u Leu 465 G u Thr Gy Leu Asn Gy 355 Val Gy Al a G n Tyr 435 Ser Asn Leu Al a Phe 515 G n 325 Thr Val G u Asp Al a 405 Leu Ser Leu Pr o SIle 485 Ser Val Pr o SIle Ser Ser 375 Phe Gy G u Asp Asn 455 Ar g Tyr Hi s Ser Thr G n Asp 360 G u Ser Hi s G u G u 440 Lys Al a Tr p Ser Ar g 520 Thr Asp 345 Ser Pr o Asp Leu SIle 425 Al a Al a Al a Pr o Ar g 505 Ser 315 Phe Leu Val Ar g Al a 395 G u G n Lys Leu Sy 475 G n Thr Asn G u Asp Asp 365 Al a Pr o G u Sy SIle 445 Ser Cys Ar g Ar g Val 525 Val Al a 350 Al a Al a Ar g Leu Leu 430 Sy Val Ser Ar g Sy 510 Sy Al a 335 let et Al a Ar g Asn 415 Phe Al a Thr Lys Sy 495 Asn Ser <210> 335 <211> 1632 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1609) <223> RXA00032 <400> 335 cacttgctga agacgcccac atcgaagacc ttgcagatgt aaacgcaaac gcctaactgt ttttcgagct aaacccatcc ttgaaaggat cttttccacc atg aac acc cca ctc IVet Asn Thr Pro Leu 1 cag ctc aac act gaa aac ctg cag gaa atc gct tcg act tcc gga gtg G n Leu Asn Thr G u Asn Leu n G u lie Al a Ser Thr Ser G y Val 15 cag atc cca gcg ttc aac cgc gct gac gtc gcc ccg ggc att gtc cac Page 425 On lie Pro Ala Phe Asn Ar g ttc Phe gaa G u ggt G y gat Asp caa G n cca Pr o t cc Ser gat Asp 150 cag G n gcc Al a at g Met ttc Phe gaa G u 230 gaa G u gat Asp gat Asp gtt Val at g Met at g Met ctt Leu at c SIle cgg Ar g 120 acg Thr gac Asp ggc G y ttg Leu tgc Cys 200 gcc Al a aac Asn acc Thr tgg Tr p ttc Phe 280 gga G y gag G u t cc Ser acc Thr gga Gy gtt Val act Thr acc Thr act Thr 170 t cc Ser aac Asn gca Al a gcc Al a ggc Gy 250 gt g Val cag G n tt c Phe ggc G y gat Asp 75 ct g Leu t cc Ser gca Al a gaa G u aac Asn 155 t cc Ser cga Ar g at c SIle cat Hi s tt c Phe 235 gac Asp gtt Val ggc G y cat Hi s aag Lys gt g Val acc Thr at c SIle acc Thr ggc G y 140 cct Pr o act Thr aaa Lys caa G n t cc Ser 220 ccc Pr o cgc Ar g tct Ser cgc Ar g Gene Al a cgc Ar g 45 gcc Al a cgc Ar g act Thr at t SIle ct c Leu 125 gga G y cga Ar g ttg Leu gaa G u ggc G y 205 gt g Val aac Asn gat Asp gaa G u ccc Pr o 285 Seq Asp 30 gct Al a ttg Leu at g Met aaa Lys gac Asp 110 gcg Al a t ac Tyr at c SIle cag G n t ca Ser 190 aac Asn tct Ser t cc Ser gac Asp gat Asp 270 gcg Al a luence 24-2-06. txt Val Ala Pro Gy I le Val Hi s caa G n tgg Tr p gat Asp cct Pr o gt g Val gac Asp at c SIle gct Al a 160 ttc Phe tct Ser gat Asp gag G u gt g Val 240 aag Lys acc Thr gag G u gcg Al a ggc G y gcc Al a gat Asp ttc Phe t cc Ser gat Asp 145 gac Asp ttt Phe acg Thr ct g Leu ct c Leu 225 gac Asp gaa G u caa G n gag G u at g bet at c SIle ct g Leu gga G y gct Al a at c SIle 130 ccg Pr o cgc Ar g ggg G y cca Pr o gct Al a 210 ggc G y cgc Ar g at c SIle tgg Tr p gtt Val 290 t ac Tyr at c SIle gcc Al a act Thr ccc Pr o 115 cgc Ar g gcg Al a gaa G u ttg Leu ttt Phe 195 aag Lys gaa G u at c SIle ggc G y gt c Val 275 ggc G y ct c Leu ggc G y agc Ser ctt Leu 100 gag G u att lle aca Thr gcc Al a at c SIe 180 acc Thr cgt Ar g tgg Tr p acc Thr t ac Tyr 260 ct c Leu gtg Val gtc gtc tcc gac gtg gag cct tat gaa tta atg aag ctg cgc ctg ctc Page 426 1027 Val Val Ser Asp Val G u Pro 295 300 Gene Sequence 24 Tyr G u Leu IVet -2-06. txt aac Asn 310 cac Hi s ct g Leu ggt Gy aac Asn gac Asp 390 gca Al a cgc Ar g gac Asp gat Asp gat Asp 470 cat Hi s t cc Ser gt c Val t ac Tyr gat Asp 360 gca Al a at t SIle cgc Ar g gca Al a ttg Leu 440 ttg Leu gaa G u cgt Ar g cag G n gac Asp 330 gag G u gat Asp aaa Lys aag Lys gt c Val 410 ggc gag G u ttc Phe ttc Phe gcg Al a 490 gga G y 315 gt c Val cgc Ar g gct Al a gac Asp tgg Tr p 395 aca Thr acc Thr cgc Ar g at c SIle acc Thr 475 gaa G u ctt Leu at g Net gaa G u t at Tyr acc Thr 380 ct g Leu ctt Leu gac Asp gt c Val cgc Ar g 460 aag Lys gca Al a tgc Cys gcg Al a gcc Al a cga Ar g 365 gt a Val ttg Leu tct Ser gag G u caa G n 445 gac Asp gca Al a acc Thr t ac Tyr gat Asp acc Thr 350 cgc Ar g ccg Pr o cca Pr o gca Al a cag G n 430 gaa G u cgt Ar g t ac Tyr at c SIle ggc G y 320 cgc Ar g acc Thr ct c Leu ct g Leu gt a Val 400 at c l Ie aac Asn gca Al a at c SIle gag G u 480 gca Al a Lys 305 cac Hi s ttc Phe ct c Leu at c SIle tgt Cys 385 cgc Ar g gt c Val cca Pr o t ca Ser ttc Phe 465 aca Thr ctt Leu Leu ttg Leu cag G n aag Lys gcg Al a 370 gcg Al a gaa G u gca Al a at a SIle gga G y 450 gga G y ct g Leu ctt Leu gct Al a gat Asp gaa G u 355 cga Ar g gaa G u aac Asn t cc Ser aag Lys 435 aat Asn gac Asp t cc Ser acg Thr G y ttc Phe 340 ctt Leu ttc Phe t cc Ser ct c Leu tgg Tr p 420 at t SIle cgc Ar g ttg Leu t cc Ser cag G n 500 \rg Leu Leu cac Hi s 325 ct c Leu cca Pr o ggc G y t cc Ser gca Al a 405 gcg Al a gtt Val acc Thr gt c Val ctt Leu 485 gt a Val 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1632 act gt c Thr Val taaatccgtt gcgcgctagg gtt <210> 336 <211> 503 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 336 IVet Asn Thr Pro Leu n Leu Asn Thr u Asn Leu On u lie Ala 1 5 10 Ser Thr Ser y Val n Ile Pro Ala Phe Asn Arg Ala Asp Val Ala 25 Page 427 Pro Gy IIe Val His Phe y Gene Sequence 24-2-06.txt Val G y Gy Phe His Arg Ala His n 40 Al a Gy Al a Asp Phe Ser Asp 145 Asp Phe Thr Leu Leu 225 Asp G u G n G u Lys 305 Hi s Phe Leu SIle Cys 385 Tyr SIle Al a Thr Pr o 115 Ar g Al a G u Leu Phe 195 Lys Su I e Gy Val 275 Gy Ar g Al a Asp G u 355 Ar g G u Asn vet G n Asp Asp Val G u Leu 165 Thr SIle Phe Val Pr o 245 I le G u G n Leu Hi s 325 Leu Pr o Sy Ser Leu Val Hi s Lys Al a Leu 135 Phe Al a Al a Ser Leu 215 Asn Thr Al a Al a Val 295 Al a vet Al a Val Al a 375 Ar g let et Leu SIle Ar g 120 Thr Asp Sy Leu Cys 200 Al a Asn Thr Tr p Phe 280 Ser Ser Val Tyr Asp 360 Al a SIle Asn Pr o Tyr SIle 105 Al a Val Hi s Asp SIle 185 Asp Phe Val Asp Pr o 265 Thr Asp Hi s Hi s bet 345 Leu Val Pr o G u Ser Thr 90 Sy Val Thr Thr Thr 170 Ser Asn Al a Al a Gy 250 Val G n Val G n Asp 330 G u Asp Lys Sy Asp Leu Ser Al a G u Asn 155 Ser Ar g SIle Hi s Phe 235 Asp Val Sy G u Sy 315 Val Ar g Al a Asp Al a Ar g Thr SIle Leu 125 Sy Ar g Leu G u Sy 205 Val Asn Asp G u Pr o 285 Tyr Cys Al a Al a Ar g 365 Val Asp Ar g Al a Tyr G n Asn Val Thr 175 Sy Sy Ser let SIe 255 Phe Tyr Leu Phe Thr 335 Pr o G n Ar g Lys Trp 395 Leu Leu Pro Val Val 400 Page 428 Ar g Val Pr o Ser Phe 465 Thr Leu Asn Ser Lys 435 Asn Asp Ser Thr Al a 405 Al a Val Thr Val Leu 485 Val Al a Ar g Asp Asp Asp 470 Hi s Thr Gy Tyr Ar g SIle 455 Al a Asp Val Gene Sequence 24-2-06.txt Arg Asp Val Thr Leu Ser Ala 410 Al a G u G y Thr Asp G u G n 425 430 Leu Ser G u Arg Val G n G u 440 445 Leu Ser Phe lie Arg Asp Arg 460 G u Pro Phe Thr Lys Ala Tyr 475 Arg y Ala u Ala Thr lie 490 Al a 415 Sy Asn Sy Ser Asp 495 <210> 337 <211> 1098 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1075) <223> RXA02528 <400> 337 ctggcgcggt atcgattaca atctggttgg ttatcggtgg gactctggta atcgaaaatt aaaggtaagg gggtgtggag tttgtggatg aatgtcatcg agc Ser tcg Ser aaa Lys gcc Al a ccg Pr o cag G n agt Ser aag Lys gca Al a cag G n gcg Al a ttg Leu ct g Leu cag G n gtt Val tct Ser gcg Al a cgg Ar g aat Asn gct Al a cag G n gct Al a ggc Sy gct Al a gaa G u gag G u 75 gct Al a gac Asp gag G u aag Lys aaa Lys cat Hi s t at Tyr ggc G y gt g Val tta Leu ggg Sy gag G u 45 cgc Ar g t at Tyr t at Tyr gt g Val gct Al a t at Tyr ct g Leu agt Ser tcg Ser caa G n at g 1et 110 gga Sy 15 gt g Val aac Asn gcc Al a gaa G u at g Net 95 gaa G u gt c Val t cc Ser t at Tyr ttg Leu tcg Ser 80 ct g Leu tcg Ser t ca gca aaa t cg Ser Ala Lys Ser tat gcc aca gcc Tyr Ala Thr Ala cag acg cgg gag Sn Thr Arg u cca aat cag ctg Pro Asn G n Leu ggg gtc att gtt Sy Val lie Val cag act att cag Sn Thr Ile n gcg gag gcc aac Ala G u Ala Asn 100 atc tcg att caa Ile Ser Ile n 115 Page 429 Gene Sequence 24-2-06.txt gct gca gga att atc cac gtt Al a Al a G y I I e I I e Hi s Val 120 gga atc ccc atg gtg cag ttg G y I e Pro ebt Val G n Leu 135 140 cct cgg gtg ttg tgt gat gat Pro Arg Val Leu Cys Asp Asp 150 155 tcg gtg ctg ggc ggc agc gga Ser Val Leu Gy y Ser y 170 gaa tca ctt tcc acc acg cag G u Ser Leu Ser Thr Thr G n 185 gcg tcg ata tat ggg gct gag Ala Ser Ile Tyr Gy Ala u 200 gtc gaa tct ggc gaa gag atg Val Gu Ser Gy Gu Gu IVet 215 220 ccc gat gca ttg att gtg gcg Pro Asp Ala Leu Ile Val Ala 230 235 cgt gct ttt act cgc ctg aat Arg Ala Phe Thr Arg Leu Asn 250 ggt ggt tat gac gat cct gag G y G y Tyr Asp Asp Pro G u 265 acc acg ttt gtt cca ccg cat Thr Thr Phe Val Pro Pro His 280 ttg ttg gta gat ctg att gaa Leu Leu Val Asp Leu Ile G u 295 300 gtt ttg cag ggg cag gtg atc Val Leu n Gy n Val lie cca gtg gtc ggc tca att Pro Val Val Gy Ser lie gct cct Ala Pro gaa G u 125 act Thr gag Gu at g bet gaa Gu gt g Val 205 gct Al a tct Ser gt c Val tgg Tr p gag G u 285 aat Asn ctt Leu cgt Ar g gct Al a aac Asn cga Ar g 190 acg Thr cag G n cct Pr o cgc Ar g t ac Tyr 270 gag G u ccc Pr o cgg Ar g ggt Gy ggg Gy at t SIle 175 at g bet ttc Phe gt g Val cgg Ar g gtt Val 255 agc Ser at g et gaa G u ggg Gy gaa G u ttt Phe 160 gct Al a cgc Ar g cat Hi s gt g Val ct g Leu 240 ccc Pr o ttt Phe ggg Gy ctt Leu tcg Ser 320 ttg Leu 145 ttt Phe gct Al a ggt Gy ttt Phe ttt Phe 225 at g Net cac Hi s gt c Val aaa Lys ccc Pr o 305 agc Ser 130 ggt Gy cag G n ttg Leu at t SIle ggc Gy 210 aac Asn gct Al a gat Asp ggc Gy gag G u 290 acc Thr aca Thr cct Pr o ct g Leu gtt Val agt Ser 195 cac Hi s aac Asn ggg Gy gt g Val gcg Al a 275 gcc Al a ggc Gy cat Hi s ggt Gy acc Thr ggt Gy 180 cat Hi s t at Tyr ggc Gy gt g Val gt g Val 260 ggg Gy gt g Val gat Asp t cc Ser 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1098 310 315 tagaattgcc caaatgtcat caa <210> 338 <211> 325 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 338 Net Ser Al a Lys Ser Ser Leu Lys G u Val Al a G u Leu Al a G y Val 1 5 10 Gy Tyr Ala Thr Ala Ser Arg Ala Leu Ser y Lys y Tyr Val Ser 25 Pro G n Thr Arg G u Lys Val G n Ala Ala Ala Lys G u Leu Asn Tyr Page 430 ene Sequence 24- 2- 06. txt ValI ValI Le u ValI Le u Ser Le u 145 Ph e A a G31y Ph e Ph e 225 vb t Hi s ValI Ly s Pr o 305 Ser As n ValI Th r O31u Ser 115 A a Pr o Le u ValI Ser 1 95 Hi s As n G31y ValI A a 275 A a G31y Hi s Le u ValI O31n As n O31n O31u Ph e O31u 1 65 O31u A a Ser Le u vb t 245 le le Ar g ValI O31y 325 A a Pr o 70 O31n Ser A a O31y Pr o 150 Ser O31u A a ValI Pr a 230 Ar g O31y Th r Le u ValI 310 Ly s As p As p ValI A a I Ie 135 Ar g ValI Ser Ser O31u 215 As p A a O31y Th r Le u 295 Le u A a Le u Le u O31n O31y 120 Pr a ValI Le u Le u I Ie 200 Ser A a Ph e Ty r Ph e 280 ValI O31n Ar g Ser Ala Leu Leu Ar g O31u Ser Ly s A a 1 li1e vb t Le u G31y Ser 1 Ty r G31y Le u Th r As p 265 ValI As p G31y As n A a 90 O31n I Ie ValI cys O31y 1 70 Th r O31y Ou le Ar g 250 As p Pr a Le u O31n (31u A a As p Hi s O31n As p 1 Ser Th r A a O31u ValI 235 Le u Pr a Pr a I Ie ValI 315 Hi s Ty r O31y ValI ValI Le u 140 As p O31y O31n O31u vb t 220 A a As n O31u Hi s O31u 300 li1e Ty r Ty r ValI Pr a 125 Th r O31u vb t O31u ValI 205 A a Ser ValI Tr p O31u 285 As n Le u (31u vb t O31u ValI O31y O31y I Ie 1 vb t Ph e ValI Ar g ValI 255 Ser vb t O31u O31y <210O> 339 <21 1> 1246 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1246) <223> RXN00316 <400> 339 Page 431 Gene Sequence 24- 2- 06. txt agcgcatgat cgatgcctgc gagtccgcag gggtccaact tggcgtgctc ttccagcgcc gcttctggcc cgcggctcaa aaaatgaaaa aggagccgtc atg ggc caa tgc acg Met Gy G n Cys Thr gt a Val gga Gy cac Hi s ttc Phe gac Asp at t SIle gt g Val ggt Gy aac Asn cat Hi s 150 ctt Leu cga Ar g agc Ser t ca Ser gat Asp 230 ctt Leu tgg Tr p at c SIle t ac Tyr gcc Al a gcc Al a gga Gy gac Asp 120 ccc Pr o caa G n cga Ar g cac Hi s cca Pr o 200 caa G n ct a Leu cga Ar g gcc Al a ctt Leu aac Asn gcc Al a acc Thr aag Lys agg Ar g cca Pr o tgc Cys gct Al a 170 agc Ser gcc Al a ggt Gy cgc Ar g cat Hi s ggc Gy t ac Tyr ttc Phe gt g Val gca Al a gcc Al a at t SIle cgc Ar g 140 gat Asp cga Ar g gaa G u ct c Leu gca Al a 220 gca Al a t cc Ser ggt Gy tgg Tr p 45 aaa Lys cgt Ar g gag G u acc Thr gtt Val 125 gga Gy ccc Pr o agg Ar g agt Ser ttt Phe 205 cct Pr o gct Al a t at Tyr gga Gy ct g Leu cac Hi s ttt Phe cca Pr o at g Net 110 cgc Ar g tct Ser gt a Val ccg Pr o tct Ser 190 gat Asp tta Leu gcc Al a t ac Tyr 15 gt g Val ttg Leu ggc Gy gaa G u gca Al a 95 acg Thr agt Ser tta Leu t ca Ser ccc Pr o 175 cct Pr o ct a Leu t cc Ser act Thr aca Thr ct c Leu ggc Gy gac Asp tcg Ser 80 ct c Leu at c SIle gaa G u t cc Ser cac Hi s 160 acc Thr tgg Tr p acg Thr t cc Ser ggt Gy 240 1 gca Al a at g bet gaa G u aac Asn ggc Gy ggc Gy ct g Leu aac Asn caa G n 145 cgc Ar g act Thr tgt Cys gaa G u ct g Leu 225 ttc Phe acc Thr act Thr ccc Pr o at c SIle gcg Al a gca Al a gaa G u gac Asp 130 tgc Cys cca Pr o gat Asp ct a Leu gct Al a 210 t cc Ser t cc Ser cca Pr o caa G n gt g Val gaa G u ttg Leu caa G n ttc Phe 115 acc Thr cga Ar g gat Asp cac Hi s cga Ar g 195 ttt Phe aca Thr ttt Phe tgg Tr p gcc Al a gaa G u gt c Val gcc Al a gt g Val 100 cct Pr o cgt Ar g tct Ser cgc Ar g cgg Ar g 180 at c SIle aaa Lys cca Pr o gt c Val cga Ar g at c SIe gtt Val gaa G u aca Thr cag G n gaa G u cga Ar g ttc Phe aga Ar g 165 ccg Pr o agc Ser acg Thr cct Pr o ggt Sy 245 ctg cgc gtc atc gca gt a Leu Arg Val Ile Ala Val acc ccc aac Thr Pro Asn gaa cgt gt a G u Arg Val Page 432 tat gac ctt tcc Tyr Asp Leu Ser 250 cca gga tcc cca ctg ctg Pro y Ser Pro Leu Leu 265 gcc ctg tat gtg ctc gac Ala Leu Tyr Val Leu Asp 280 acc cgc gag gcc tgg ctt Thr Arg G u Ala Trp Leu 295 gct aaa acc ttc acc atc Ala Lys Thr Phe Thr Ile 310 315 acc gac acg atc ggt gcc Thr Asp Thr lie Gy Al a 330 acc cca gcc cta gag cca Thr Pro Ala Leu G u Pro 345 cag gca gca gca atc gcc G n Ala Ala Ala IIe Al a 360 acc ttg cac atg gcc agg Thr Leu His IVet Ala Arg 375 <210> 340 <211> 382 <212> PRT <213> Corynebacter i um gl <400> 340 Met Gy G n Cys Thr Val 1 5 Ala Thr Pro Trp Arg G y Met Thr n Ala Ile His G u Pro Val G u Val Phe Asn Ile u Val u Asp 70 Sy Ala Leu Ala Thr Ile Sy Al a n Val n Val 100 Leu u Phe Pro Gu y 115 Asn Asp Thr Arg Arg Asn 130 gct Al a act Thr ccc Pr o 300 gcc Al a at g Met at c SIle aga Ar g ttc Phe 380 Gene Sequence 24-2-06.txt 255 gca acc caa caa gcg ttg aaa Ala Thr G n G n Ala Leu Lys 270 275 gaa ttc cta cag gta aac gca G u Phe Leu G n Val Asn Ala 285 290 gca cta gaa gcc gcc gga gca Al a Leu G u Al a Al a G y Al a 305 gcc ggt gat gac aac att gcg Al a y Asp Asp Asn Ile Al a 320 gtt gac gat gcc cgt gat ttc Val Asp Asp Ala Arg Asp Phe 335 tct tac cgc agc gtg cat tcc Ser Tyr Arg Ser Val His Ser 350 355 gac tcc ggc gga aaa gtc gtg Asp Ser Gy y Lys Val Val 365 370 gga gcc G y Al a 260 gaa G u gac Asp ct g Leu ccc Pr o gga Gy 340 at t SIe gcg Al a 931 979 1027 1075 1123 1171 1219 1246 ut am cum Ala Leu Tyr Thr Trp Al a Tyr Ile Asp 40 G y Tyr Thr 55 Ser Al a Val Ser Al a Thr Vet G y Thr 105 Thr Asp G y 120 His Pro Ile 135 Arg G u Hi s 10 Al a Asp G y Leu Leu Tyr Asn Ser Phe Al a Thr Val Thr Al a Al a 90 Lys G y Al a Arg Leu Ile Pro Pro Ar g 140 Page 433 Ser Gy Tr p Lys Ar g G u Thr Val 125 Gy Tyr Val Leu Gy G u Al a Thr Ser Leu Sn Cys Arg 145 Ser Phe His His 150 Gene Sequence 24- 2- 06. t xt Sn Arg Cys Phe Asp Pro Val Ser His Ar g Thr Cys G u Leu 225 Phe Val Al a Val Al a 305 Asn Ar g Val Lys Asp Hi s Ar g 195 Phe Thr Phe Asp Lys 275 Al a Al a Al a Phe Ser 355 Val Ar g 165 Pr o Ser Thr Pr o Sy 245 Ser Thr Thr Sy Leu 325 Val Pr o Leu Ar g Ser Ser Asp 230 Leu Pr o Al a Thr Al a 310 Thr Thr G n Tyr Cys Hi s Ar g 215 G n Ar g Sy Leu Ar g 295 Lys Asp Pr o Al a Leu 375 Ar g Hi s Pr o 200 G n Leu Val Ser Tyr 280 G u Thr Thr Al a Al a 360 Cys G n 185 Pr o SIle Val SIle Pr o 265 Val Al a Phe SIle Leu 345 Al a Al a 170 Ser Al a Sy Ar g Al a 250 Leu Leu Tr p Thr Sy 330 G u lie G n Ser Sy Leu Leu 235 Val Leu Asp Leu SIle 315 Al a Pr o Al a Ar g G u Leu Al a 220 Al a Thr Al a Thr Pr o 300 Al a let SIle Ar g Ar g Ser Phe 205 Pr o Al a Pr o Al a G u 285 Al a Al a Val Ser Asp 365 Pr o 175 Pr o Leu Ser Thr G u 255 G n Leu G u Asp Asp 335 Ar g Sy Al a Asp Thr His Met Ala Arg Phe G y Al a 380 <210> 341 <211> 412 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(412) <223> FRXA00309 <400> 341 agcgcatgat cgatgcctgc gagtccgcag gggtccaact tggcgtgctc ttccagcgcc gcttctggcc cgcggctcaa aaaatgaaaa aggagccgtc atg ggc caa tgc acg IVet Gy n Cys Thr 1 gta gcg ctt tac cga gag cat tcc tat tac aca gca acc cca tgg cga Val Ala Leu Tyr Arg G u His Ser Tyr Tyr Thr Ala Thr Pro Trp Arg 15 gga acc tgg gca gcc gat ggc ggt gga gtg ctc atg act caa gcc atc Page 434 Gene Sequence 24-2-06.txt Sy Thr Trp Ala Ala Asp G y y y Val Leu Iet Thr n Ala lle 30 cac tac atc gat ctt ttg tac tgg ctg ttg ggc gaa ccc gtg gaa gtt 259 His Tyr Ile Asp Leu Leu Tyr Trp Leu Leu Gy G u Pro Val G u Val 45 ttc ggc tac acc aac tcc ttc aaa cac ggc gac aac atc gaa gtc gaa 307 Phe G y Tyr Thr Asn Ser Phe Lys His G y Asp Asn IIe G u Val G u 60 gac agc gcc gtt gcc act gtg cgt ttt gaa tcg ggc gcg ttg gcc aca 355 Asp Ser Ala Val Ala Thr Val Arg Phe u Ser Gy Ala Leu Ala Thr 75 80 att tca gcc acc acc gcc gca gag cca gca ctc ggc gca caa gtg cag 403 Ile Ser Ala Thr Thr Al a Al a u Pro Ala Leu y Al a n Val G n 95 100 gtg atg gga 412 Val eVt G y <210> 342 <211> 104 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 342 IVet G y G n Cys Thr Val Ala Leu Tyr Arg G u His Ser Tyr Tyr Thr 1 5 10 Ala Thr Pro Trp Arg G y Thr Trp Ala Ala Asp G y G y G y Val Leu 25 IVet Thr G n Al a lIe Hi s Tyr I e Asp Leu Leu Tyr Trp Leu Leu G y 40 G u Pro Val G u Val Phe G y Tyr Thr Asn Ser Phe Lys His G y Asp 55 Asn Ile G u Val G u Asp Ser Ala Val Ala Thr Val Arg Phe u Ser 70 75 G y Al a Leu Al a Thr I e Ser Al a Thr Thr Al a Al a G u Pro Al a Leu 90 Sy Al a n Val n Val [et y 100 <210> 343 <211> 558 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(535) <223> RXN00310 <400> 343 ttgcgggatt catcatcggc gcaatcgcac tgtctgccgc agttattttg accaccaagg aaaccgcctt caccaagctt gaagatctag ggaagaaata atg tct gac aag atc 115 Iet Ser Asp Lys Ile 1 Page 435 Gene Sequence 24-2-06.txt tgg aaa Trp Lys gaa gca u Al a gtg gat Val Asp ccc agt Pro Ser gtc gca Val Ala gct gct Al a Al a gaa ct c G u Leu gtc caa Val G n aaa at g Lys IVet 135 gt c Val gtt Val ggt Sy ttc Phe gt c Val gcc Al a gat Asp ctt Leu 120 aaa Lys at c I e gca Al a aaa Lys tct Ser acc Thr gga G y gca Al a gt g Val at c SIle at c l Ie gca Al a gt c Val cca Pr o 75 gt g Val cag G n ct c Leu ggt tgc ggt Sy Cys y ccc ggc gca Pro G y Al a t cg gaa acc Ser G u Thr 45 gat gag at c Asp G u I le 60 cat cca acc Hi s Pro Thr cac gtg ctt Hi s Val Leu cgc atg at c Arg IVet lie 110 ttc cag cgc Phe G n Ar g 125 gca Al a 15 gaa G u gca Al a ct c Leu cac Hi s tgt Cys 95 gat Asp cgc Ar g atc agc lie Ser gtc agc Val Ser gcg aaa Al a Lys gcc t cc Al a Ser gaa aca G u Thr 80 gag aag G u Lys gcc tgc Al a Cys ttc tgg Phe Trp cga aac cat atc Arg Asn His Ile gca gtc tgt gat Ala Val Cys Asp tat gga att tct Tyr G y Ile Ser ggg gtg gac att Gy Val Asp Ile gtg gtc ctc gcc Val Val Leu Ala cca atc gcc atc Pro Ile Ala lle 100 gag tcc gca ggg Su Ser Ala G y 115 ccc gcg gct caa Pro Ala Ala G n 130 t agcgct t t a gag ccg tca tgg gcc aat gca Su Pro Ser Trp Ala Asn Ala 140 ccgagagcat tcc <210> 344 <211> 145 <212> PRT <213> Corynebacter i um gl utam cum <400> 344 IVet Ser Asp Lys lie Trp Lys Val y 1 5 Ser Arg Asn His Ile u Ala Val n 25 Ser Ala Val Cys Asp Val Asp Gy Al a 40 Lys Tyr Gy lie Ser Pro Ser Phe Thr Ser y Val Asp Ile Val Ala Val Cys 70 Thr Val Val Leu Ala Al a Ala Ala Al a Lys Pro lie Ala lie u Leu Asp Ser 100 105 Cys u Ser Ala y Val n Leu y lie le 10 Ala I e I Lys Al a Ser Val Thr Pro I 75 Sy Val I 90 Al a n Val Leu I Page 436 Cys Sy G u G u Pr o Val Met G n Sy Al a Thr SIle Thr Leu SIle 110 Ar g Al a G u Al a Leu Hi s Cys Asp Ar g SIe Val Al a Al a G u G u Al a Phe Gene Sequence 24-2-06.txt 115 120 125 Trp Pro Ala Ala On Lys IVet Lys Lys u Pro Ser Trp Ala Asn Ala 130 135 140 Ar g 145 <210> 345 <211> 558 <212> DNA <213> Cor ynebact er i um gl ut ari cum <220> <221> CDS <222> (101)..(535) <223> FRXA00310 <400> 345 ttgcgggatt catcatcggc gcaatcgcac tgtctgccgc agttattttg accaccaagg aaaccgcctt caccaagctt gaagatctag ggaagaaata tgg Tr p gaa Gu gt g Val ccc Pr o gt c Val gct Al a gaa G u gt c Val aaa Lys gt c Val gtt Val ggt Gy ttc Phe gt c Val gcc Al a gat Asp ctt Leu 120 aaa Lys ggc atc at c G y l e le cag gca at c G n Ala lle gcg aaa gca Al a Lys Al a acg tct gt c Thr Ser Val tgc acc cca Cys Thr Pro 75 gcc gga gt g Al a y Val t cc gca cag Ser Al a G n 105 ggc gtg ct c G y Val Leu aag gag ccg Lys G u Pr o ggt Gy ccc Pr o tcg Ser gat Asp cat Hi s cac Hi s cgc Ar g ttc Phe ggt Gy gca Al a 30 acc Thr at c SIle acc Thr ctt Leu at c SIle 110 cgc Ar g gca Al a 15 gaa Gu gca Al a ct c Leu cac Hi s tgt Cys 95 gat Asp cgc Ar g at c l Ie gt c Val gcg Al a gcc Al a gaa Gu 80 gag G u gcc Al a ttc Phe at g t ct et Ser 1 agc cga Ser Arg agc gca Ser Al a aaa t at Lys Tyr tcc ggg Ser G y aca gt g Thr Val aag cca Lys Pro tgc gag Cys u tgg ccc Trp Pro 130 gac aag atc Asp Lys Ile aac cat atc Asn His Ile gtc tgt gat Val Cys Asp gga at t t ct G y Ile Ser gtg gac att Val Asp Ile gt c ct c gcc Val Leu Al a atc gcc atc I e Al a le 100 tcc gca ggg Ser Al a G y 115 gcg gct caa Al a Al a G n tca tgg gcc aat gca cgg Ser Trp Ala Asn Ala Arg 140 145 t agcgct t t a ccgagagcat tcc <210> 346 <211> 145 <212> PRT Page 437 Gene Sequence 24 <213> Corynebacter i um gl ut am cum <400> 346 IVet Ser Asp Lys Ile Trp Lys Val Gy Ile Ile 1 5 10 Ser Arg Asn His Ile u Ala Val n Ala lie 25 Ser Ala Val Cys Asp Val Asp Gy Ala Lys Ala 40 Lys Tyr Gy lie Ser Pro Ser Phe Thr Ser Val 55 Ser y Val Asp Ile Val Ala Val Cys Thr Pro 70 Thr Val Val Leu Ala Al a Ala Ala Ala y Val 90 Lys Pro lie Ala lie u Leu Asp Ser Ala n 100 105 Cys u Ser Ala y Val G n Leu Gy Val Leu 115 120 Trp Pro Ala Ala G n Lys IVet Lys Lys G u Pro 130 135 Ar g 145 <210> 347 <211> 1342 <212> DNA <213> Cor ynebact er i um gl ut ai cum <220> <221> CDS <222> (101)..(1342) <223> RXA00041 <400> 347 atgaagcagc agcatccaag ctggaaaacg ctgatcacta agctgcgcta gaaacaaaaa ggaaagtagt gtgtggggct -2-06. txt Sy Cys Pro G y Ser Gu 1 Asp u I His Pro 1 His Val L Arg Iet I 1 Phe G n 125 Ser Trp 140 3y l a Thr le Thr eu le r g a Al a G u Al a Leu Hi s Cys Asp Ar g Asn t cc Ser aat Asn t ac Tyr gct Al a cac ttg Leu ccc Pr o cac Hi s acc Thr ccc cct Pr o gga Gy cca Pr o acg Thr gct gcg Al a at g bet ggt Gy ccg Pr o ctt t ac Tyr t ac Tyr ttc Phe ttg Leu t ac gt g Val gat Asp ttc Phe gga gty gat act cct Thr Pro 15 ggc gat Sy Asp gca cca Ala Pro ccg cag Pro G n gca t cc Page 438 ccgtctcatg gagcaat t aa atg cac aca gaa ctt IVet His Thr G u Leu 1 ccg cag ggc agg ctc Pro G n y Arg Leu acc ctc cac gtc tac Thr Leu His Val Tyr aag cgc acc ggc tgg Lys Arg Thr y Trp cga ttg cag tgg acg Arg Leu G n Trp Thr tat gac ctg gat gga His Leu Pro Asp Ala Leu Tyr Gene Sequence 24-2-06.txt Pro Asp Ala Ser Tyr Asp Leu Asp tgc Cys t ac Tyr ct c Leu cgt Ar g ccc Pr o 150 at g let t ac Tyr ttt Phe ggt Sy gaa G u 230 gac Asp gga Gy gga Gy gca Al a gcg Al a 310 ct g Leu t cc Ser ggc Gy gaa G u 120 cct Pr o t ac Tyr ctt Leu tcg Ser ct c Leu 200 t ac Tyr ggc G y at c SIle gt c Val agc Ser 280 aac Asn gat Asp gt g Val gga G y ct a Leu gag G u aat Asn gat Asp gcc Al a 170 gat Asp gat Asp tgg Tr p gat Asp gat Asp 250 ggc Gy ct g Leu tct Ser cac Hi s cgc Ar g 330 gcc Al a aaa Lys gac Asp ccg Pr o ccc Pr o 155 caa G n ctt Leu gca Al a gaa G u ct c Leu 235 gag G u aag Lys gat Asp gat Asp cca Pr o 315 aag Lys gt a Val at t SIle cca Pr o ctt Leu 140 at g let cgc Ar g gaa G u caa G n tgc Cys 220 gac Asp gtt Val ctt Leu ttc Phe gcc Al a 300 aca Thr ctt Leu ttt Phe gac Asp act Thr 125 at c SIle at c SIle gaa G u aac Asn cct Pr o 205 ccc Pr o gt g Val act Thr gaa G u ggc Gy 285 tgg Tr p gtt Val cat Hi s act Thr ggc Gy 110 ggg Gy gac Asp agc Ser aac Asn tgg Tr p 190 ggt G y aac Asn ct g Leu cac Hi s gga Gy 270 cat Hi s ct c Leu gca Al a ttg Leu ggc G y cgc Ar g at g Net ccc Pr o gat Asp 160 acc Thr ttc Phe gct Al a ttt Phe ttc Phe 240 gca Al a acc Thr ttc Phe ggc Gy gaa G u 320 aac Asn aca Thr cgc Ar g ggc Gy gcc Al a 145 ggt Gy ggt Gy t cc Ser cct Pr o acg Thr 225 tgt Cys agc Ser ttc Phe t ac Tyr tgg Tr p 305 gga Gy cac Hi s ctt Leu gcc Al a ggc Gy 130 agc Ser gat Asp gca Al a ggt G y gat Asp 210 ctt Leu cca Pr o tct Ser cgc Ar g gca Al a 290 at g Net tgg Tr p gcg Al a aaa Lys acc Thr 115 at t SIle ggt Gy ggt Gy gcg Al a gaa G u 195 ct c Leu cgc Ar g caa G n gac Asp gt c Val 275 ccg Pr o ggg Gy gt g Val at c l Ie ctt Leu 100 caa G n cat Hi s ttc Phe tgg Tr p gtt Val 180 at c SIle gtt Val gat Asp gga G y cag G n 260 ttg Leu cag G n ct g Leu cac Hi s t at Tyr 340 ttc Phe aac Asn cgc Ar g aca Thr aaa Lys 165 ct a Leu acc Thr ccc Pr o gaa G u ttg Leu 245 tgc Cys cga Ar g gtt Val ccc Pr o tgc Cys 325 caa G n 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 gag ctt ctt ctc cca gag ggg gag tca ggg gta atc aga tct gta tta Page 439 G u ggt Sy tgg Tr p gct Al a 390 at t SIle Leu gaa G u 360 ggt Gy gt a Val at a I le Pr o gt c Val cgt Ar g cct Pr o gca Al a 410 G u cga Ar g tt g Leu ggc Gy 395 ggt Gy Gy gt a Val tct Ser 380 gaa G u gat Asp Gene Sequence 24-2-06.txt Su Ser Gy Val Ile Arg Ser Val Leu 350 355 gac atc cga ggc aat att tcc ctc gag Asp lie Arg y Asn Ile Ser Leu u 365 370 gtg gat cgt ggt ggt gat cgt cgc gta Val Asp Arg Gy y Asp Arg Arg Val 385 tta gtg atc gcg gac gat aat aca gcc Leu Val Ile Ala Asp Asp Asn Thr Ala 400 405 gga cag G y G n 1219 1267 1315 1342 <210> 348 <211> 414 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 348 Net His Thr u 1 Pro G n y Ar g Thr Leu His Val Lys Arg Thr y Arg Leu G n Trp Tyr Asp Leu Asp Thr Leu Lys Leu 100 Arg Ala Thr G n 115 G y y I I e Hi s 130 Al a Ser G y Phe 145 Sy Asp y Trp G y Al a Al a Val 180 Ser G y G u II e 195 Pro Asp Leu Val 210 Leu Ser 5 Leu Asn Tyr Tyr Trp Al a Thr Hi s 70 Sy Cys Phe Tyr Asn Leu Arg Arg Thr Pro 150 Lys IVet 165 Leu Tyr Thr Phe Pro G y Ser Leu Arg Pro 10 Asp Pro Asn y Sn His Asp Pro 40 His Thr Thr Thr Leu Pro Asp Ala Tyr Ser G y G y 90 Thr G y Asn Leu 105 Val G u Val G u 120 Ser Pro Lys Asn 135 His Tyr Arg Asp Val Leu y Al a 170 Arg Ser Thr Asp 185 Asp Leu Ser Asp 200 G y Tyr IVet Trp 215 Al a et Sy Pr o Leu Al a Lys Asp Pr o Pr o 155 G n Leu Al a Hi s Val Pr o Thr Pr o Phe Asp Thr 125 I e I e Su Asn Pr o 205 Thr Sy Al a Pr o Al a Asp Lys Leu Sy Pr o Leu 175 G u Ser G u Cys Pro Asn Leu Phe 220 Page 440 Thr Leu Ar g 225 Asp G u G u Thr 230 Gene Sequence 24-2-06.txt Sy G u Asp Leu Asp Val Leu Ile Phe Cys Ser Phe Tyr Tr p 305 Gy Hi s SIle Asn Gy 385 Asp G n Asp Val 275 Pr o Gy Val SIle Ser 355 Ser Ar g Asn Leu 245 Cys Ar g Val Pr o Cys 325 G n Leu G u Val Asp Gy Gy Al a Al a 310 Leu G u Gy Tr p Al a 390 Ar g Tyr Phe Val 295 G n Thr Leu Ser Asp 375 G u Hi s Val 265 G u Gy Asp Pr o Leu 345 Pr o Val Lys G u Lys Asp Asp Pr o 315 Lys G u Ar g Leu Gy 395 Thr G u Gy 285 Tr p Val Hi s G u Asp 365 Val Leu Tyr 255 Thr G u Val Ar g Ar g 335 Gy Ar g Ar g lie Al a Thr Phe Gy G u 320 Asn Val Gy Gy Al a 400 Thr Ala I e u Ile Thr Al a y Asp y n 405 410 <210> 349 <211> 720 <212> DNA <213> Corynebacter i um gl ut arri cum <220> <221> CDS <222> <223> RXA02026 <400> 349 cca ttt cat ata caa cca gaa aca ggt Pro Phe His lie G n Pro G u Thr G y 1 5 ctt att ttt tat aaa ggg aag tat tat Leu Ile Phe Tyr Lys G y Lys Tyr Tyr 25 tta ggc gca gta cat ggc tta aag tat Leu y Ala Val His G y Leu Lys Tyr 40 gac tta ata aac ttt aaa cct gaa ggg Asp Leu Ile Asn Phe Lys Pro G u y 55 aaa tat gac agc cat ggt gtt tat agc Lys Tyr Asp Ser His G y Val Tyr Ser 70 tta tta aat Leu Leu Asn 10 gtt tca cat Val Ser Hi s tgg tat aac Trp Tyr Asn cca ata tta Pro Ile Leu ggt agc gct G y Ser Al a 75 Page 441 gat Asp caa G n t ac Tyr aat Asn ttt Phe ccc Pr o tgg Tr p acg Thr cca Pr o gaa G u aac Asn ttc Phe agt Ser gat Asp t at Tyr gga Gy cca Pr o gat Asp act Thr aac Asn Gene Sequence 24-2-06.txt ggg cat tta tat tat atg tac aca gga aat cat cga G y His Leu Tyr Tyr IVet Tyr Thr G y Asn His Arg 90 caa cga cat gcg agt cag atg atc gca cga ttg aaa G n Arg His Ala Ser G n et Ile Ala Arg Leu Lys 100 105 gtt gaa aag ttt cca aag cca gta att agc cag caa Val G u Lys Phe Pro Lys Pro Val Ile Ser G n G n 115 120 aca agt cat ttt aga gat cct aaa gtt ttt aaa tat Thr Ser His Phe Arg Asp Pro Lys Val Phe Lys Tyr 130 135 140 tat gca atc att ggc gca caa aat aat gat cag caa Tyr Ala lie Ile G y Ala n Asn Asn Asp n G n 145 150 155 ctt tat aat act gaa gat ata att aat tgg cat tat Leu Tyr Asn Thr G u Asp lie lie Asn Trp His Tyr 165 170 aat aca gag tta gat gat ttt gga tat atg tgg gaa Asn Thr G u Leu Asp Asp Phe G y Tyr IVet Trp G u 180 185 ttt aat tta gat aat caa gat gtc ata ctt att tgt Phe Asn Leu Asp Asn G n Asp Val lie Leu Ile Cys 195 200 gaa cca aaa ggc aat cag ttc aaa aat att tat caa Su Pro Lys y Asn n Phe Lys Asn Ile Tyr n 210 215 220 ctt gga aag ttt gat att gaa aag tta aca tat gaa Leu G y Lys Phe Asp Ile G u Lys Leu Thr Tyr G u 225 230 235 <210> 350 <211> 240 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 350 Pro Phe His lie G n Pro G u Thr y Leu Leu Asn 1 5 10 Leu Ile Phe Tyr Lys G y Lys Tyr Tyr Val Ser His Leu y Ala Val His G y Leu Lys Tyr Trp Tyr Asn 40 Asp Leu Ile Asn Phe Lys Pro G u Gy Pro lie Leu 55 Lys Tyr Asp Ser His G y Val Tyr Ser G y Ser Ala 70 G y Hi s Leu Tyr Tyr IVet Tyr Thr G y Asn His Arg 90 G n Arg His Ala Ser G n IVet lie Ala Arg Leu Lys 100 105 Page 442 gat Asp gaa G u cca Pr o 125 ggt Gy ggt Gy tta Leu tgc Cys cca Pr o 205 agt Ser gac Asp 110 gaa G u gag G u cga Ar g ggt Gy cca Pr o 190 caa G n ggt Gy aat cat t gg Asn His Trp ggt Sy gga Gy aaa Lys tta Leu gaa G u 175 gat Asp ggt Gy t at Tyr cat gaa aat His G u Asn Asp G n Tyr Asn Phe Asp G u Asn Phe Ser Asp Tyr Hi s Gy Val Thr Tyr 145 Leu Asn Phe G u Leu 225 Lys 115 Hi s SIle Asn G u Leu 195 Lys Lys Pr o Ar g Gy G u 165 Asp Asn Asn Asp Lys Asp Al a 150 Asp Asp G n G n SIle 230 Pr o Pr o 135 G n SIle Phe Asp Phe 215 G u Gene Sequence 24-2-06.txt Val Ile Ser n n Pro u 120 125 Lys Val Phe Lys Tyr Gy G u 140 Asn Asn Asp On On y Arg 155 IIe Asn Trp His Tyr Leu G y 170 G y Tyr Met Trp G u Cys Pro 185 190 Val lie Leu Ile Cys Pro n 200 205 Lys Asn Ile Tyr G n Ser G y 220 Lys Leu Thr Tyr G u His G u 235 Sy Lys Leu G u 175 Asp Sy Tyr Asn <210> 351 <211> 1617 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1594) <223> RXA02061 <400> 351 taacctgcct tcacctggga ttatccaccc atgtgtgatg agacactggc gtaattgagt gaaggcagga caataaagag ggg G y ttt Phe cac Hi s ttc Phe gcc Al a aac Asn cac Hi s cct Pr o t ac Tyr gag Su gaa G u ggt Sy cca Pr o ggc G y ccc Pr o act Thr gat Asp gtt Val gag G u gtt Val t ca Ser cca Pr o gaa G u 75 gat Asp ct g Leu ct g Leu cca Pr o ttt Phe acc Thr ct g Leu at c SIle gat Asp cac Hi s tgg Tr p gtt Val ttc Phe acc gct Thr Al a 15 gac gac Asp Asp ggc ccc G y Pro gat at c Asp II e cgc gct Arg Ala 80 acc tcg Thr Ser 95 Page 443 aaatcgctac cctgaatcaa atg acg aac gtt tcc IVet Thr Asn Val Ser 1 gaa agt ggt gtt ttg Su Ser G y Val Leu acg tgg cat ttc ttc Thr Trp His Phe Phe agg tgg gcg cac caa Arg Trp Ala His G n tgc gat gac gtg cta Cys Asp Asp Val Leu ggc tca gtg gtg tcc G y Ser Val Val Ser gtt gtt ggc ccc act Val Val G y Pro Thr 100 Gene Sequence 24-2-06.txt t cc Ser ct g Leu ggc G y tgc Cys 150 t ca Ser gt a Val ccc Pr o gtt Val t ac Tyr 230 gga G y cca Pr o acc Thr ggt Sy agt Ser 310 gt c Val ctt Leu tgc Cys at c SIle aat Asn 120 gt g Val at c SIle tgg Tr p gt c Val tct Ser 200 cct Pr o gt c Val ct g Leu acc Thr t ac Tyr 280 at g et aag Lys ct c Leu gcc Al a at c SIle 360 ttg Leu gac Asp ggc G y ggt Gy at g let 170 gac Asp aac Asn at g let at t SIle ggc Gy 250 at c SIle gaa G u ggc G y gaa G u gac Asp 330 cat Hi s t ca Ser gca Al a gag G u aac Asn tgg Tr p 155 ct c Leu tcg Ser ggc Gy at t SIle gt c Val 235 cag G n gat Asp acc Thr gt a Val ggc G y 315 ggt G y gaa G u aac Asn cac atc aac His Il e ctg ggg Leu G y 125 act gat Thr Asp 140 gaa gac Gu Asp gca gtt Al a Val cca gat Pro Asp ct c t ct Leu Ser 205 cgt ctg Arg Leu 220 acc att Thr Ile ctc aac Leu Asn ttt ggc Phe G y acc at c Thr Ile 285 ggt cgt Sy Arg 300 tgg gca Trp Al a acg gtc Thr Val t cc gag Ser G u agc gca Ser Al a 365 Asn 110 ct c Leu ggt G y caa Gn act Thr gga G y 190 gga Gy cgc Ar g gaa G u ggc Gy cat Hi s 270 ggc G y ttg Leu aac Asn ttc Phe gca Al a 350 gtt Val aac at c cgt Asn lie Ar g ggc acc acc gaa G y Thr Thr G u gat Asp t at Tyr gga Gy ggc G y 175 aga Ar g tta Leu gat Asp caa G n t ca Ser 255 gat Asp t ac Tyr gac Asp gct Al a cag G n 335 gcg Al a gaa G u cca Pr o gt a Val aac Asn 160 cca Pr o gaa G u gag G u gaa G u gac Asp 240 gaa G u ttc Phe gac Asp t cc Ser at t lie 320 acc Thr gca Al a gt g Val gat Asp aag Lys 145 cgc Ar g gtt Val tgg Tr p t ca Ser gt g Val 225 ggg G y ttc Phe tct Ser ttc Phe ccc Pr o 305 tct Ser cct Pr o ggt G y gcg Al a gt c Val 130 ttc Phe gat Asp gaa G u t cc Ser gac Asp 210 gat Asp at t SIle gat Asp cgc Ar g gcc Al a 290 act Thr ttc Phe cca Pr o tgg Tr p ttg Leu 370 115 t cc Ser cgc Ar g gaa Gu gcc Al a at t SIle 195 gaa G u cat Hi s gac Asp gt g Val ccc Pr o 275 cac Hi s gag G u cca Pr o gaa G u acc Thr 355 aag Lys cga Ar g t ca Ser ggc Gy cca Pr o 180 aca Thr gtt Val gaa G u at t SIle aag Lys 260 cgc Ar g at c SIle cat Hi s cgt Ar g gga G y 340 gga G y gac Asp at c SIe ccg Pr o cac Hi s 165 aca Thr ggt G y ct a Leu at c SIe tcg Ser 245 act Thr aac Asn ttt Phe ct c Leu at t SIe 325 ttg Leu ct g Leu caa G n 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 Page 444 Gene Sequence 24-2-06.txt gaa G u gtt Val 390 gca Al a ggc G y agc Ser acc Thr gat Asp 470 cag G n gaa G u cgg Ar g ttg Leu aca Thr gt g Val 440 ggt Sy agt Ser gat Asp gct Al a at g bet gat Asp 410 gaa G u ttc Phe gca Al a gt g Val aac Asn 490 gca Al a aac Asn 395 gat Asp gtt Val aac Asn agc Ser t cc Ser 475 gaa G u aca at c act Thr Ile Thr 380 ccc aac cac Pro Asn Hi s gaa aca gat G u Thr Asp ttt gct gat Phe Al a Asp 430 aac gga cca Asn G y Pr o 445 att att cgc lie lie Ar g 460 ct a gat at a Leu Asp Ile ccg cat gaa Pro His G u cac cgc cac aat His Arg Hi s 385 gcg ggt gat Al a y Asp 400 tca ctg ttc Ser Leu Phe 415 ggc ggt at G y G y Tyr ttc agc gaa Phe Ser G u cag gaa agt G n 0 u Ser 465 gat gat ct c Asp Asp Leu 480 ggc cca gt g Sy Pro Val 495 Asn cca Pr o at t SIle gt a Val ttt Phe 450 cac Hi s act Thr aga Ar g cag ct a gt c G n Leu Val cac gcg att His Ala lle 405 gtc gtt gac Val Val Asp 420 t ca at g gca Ser IVet Al a 435 gag gt c acc G u Val Thr ttc cct gtt Phe Pro Val gcg ctc at g Al a Leu IVet 485 t aagagt tag 1267 1315 1363 1411 1459 1507 1555 1604 1617 atgcgt t cca gcc <210> 352 <211> 498 <212> PRT <213> Corynebacter i um gl utam cum <400> 352 IVet Thr Asn Val Ser y Tyr His Arg 1 5 Su Ser y Val Leu Phe Ala Pro Ala Thr Trp His Phe Phe His G n Tyr Arg 40 Arg Trp Ala His G n Phe Ala G u Arg Cys Asp Asp Val Leu Al a Pro G u G y 70 Sy Ser Val Val Ser Asn Asn Gy y Val Val y Pro Thr Ser Thr Ile G n 100 105 Arg y Thr Thr u Leu Ile Asn u 115 120 Asp Val Ser Arg Ile G y G u Val Val 130 135 Pro u I 10 Sy Val I Pro Ser I Thr Pro I Asp u Val Asp I 90 Leu Al a I Asp Gu I Sy Asn Page 445 Hi s Leu Asp Val G n Tyr SIle Gy 125 Asp Thr Asp Gy Asp Ar g Thr Asn Asp Tyr ene Sequence 24- 2- 06. txt Ly s 145 Ar g ValI Tr p Ser ValI 225 G31y Ph e Ser Ph e Pr o 305 Ser Pr o G31y A a Hi s 385 As p Ph e Ty r O31u Ser 465 Le u Ar g O31u A a I Ie 1 95 O31u Hi s As p ValI Pr o 275 Hi s O31u Pr a O31u Th r 355 Ly s O31n Hi s ValI Ser 435 O31u Ph e A a Ser Pr o Cys 150 03 y Hi s Ser 1 65 Pr o Thr Val 1 80 Thr G31y Pr o Val Leu Val 03 u I I e Ty r 230 1 1 e Ser 03 y 245 Lys Thr Pr o 260 Ar g As n Thr I I e Phe 03 y Hi s Leu Ser 310 Ar g Ilie Val 325 03 y Leu Leu 340 03 y Leu Cys Asp O31n O31u Leu Val Val 390 A a IlIe A a 405 Val Asp (1y 420 I'b t A a Ser Val Thr Thr Pr o Val Asp 470 Leu I'b t 03 n 485 Val Ilie Pro G1y G31y ValI Le u A a 215 As p Tyr Ph e As n Le u 295 Tr p Thr As p O31u G31y 375 As p Pr o Ser Ar g Thr 455 Ph e Ph e Le u Le u 1 Le u Ar g Le u ValI Ar g 265 A a As n O31u O31n I Ie 345 Pr o I Ie Ser Thr ValI 425 Tyr As p Ser O31u vb t 1 70 As p As n vb t I Ie O31y 250 li1e G u O31y O31u As p 330 Hi s Ser A a vb t As p 410 O31u Ph e A a ValI As n 490 Tr p 1 Le u Ser Gy le ValI 235 O31n As p Thr ValI G31y 315 G31y (31u As n A a As n 395 As p ValI As n Ser Ser 475 (31u (31uAsp (1n (1y As n A a Pr o Le u Ar g 220 Thr Le u Ph e Thr G31y 300 Tr p Thr Ser Ser Thr 380 Pr o (31u Ph e As n I Ie 460 Le u Pr o ValI As p Ser 205 Le u I Ie As n G31y I Ie 285 Ar g A a ValI (31u A a 365 li1e As n Thr A a G31y 445 li1e As p Hi s G31y 1 Ar g Le u As p O31n Ser 255 As p Tyr As p A a O31n 335 A a O31u Hi s A a Ser 415 O31y Ph e O31n As p O31y 495 Val Ar g Page 446 ene Sequence 24- 2- 06. txt <210O> 353 <21 1> 1305 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1282) <223> RXN01369 <400> 353 ct gcgacgga cct agcaaag gggcgct gac acaagcact g gt cagccacg acct at tcca t tgaagaaaa ggact tgt aa cgtttgctgg tgcgcggaca atg gag cta ttg gaa 11! I'bt G3 u Leu Leu G3 u ggc G31y ct c Le u tt c Ph e aac As n gcc A a gcg A a cgt Ar g ccg Pr o gct A a ac c Th r 1 50 cgc Ar g tt t Ph e ct g Le u ggc G y gcc A a gt c ValI gcg A a gca A a gga 0Gy cgc Ar g 120 ac g Th r ac c Th r at g vb t ac c Th r ac c Th r g aa G u cca Pr o gca A a g aa 0Gu ct a Le u gcc A a t ac Ty r tt c Ph e tt c Ph e ac c Th r 1 70 at c I Ie t ac Tyr t ca Ser gga G31y gcg A a aat As n 75 t ca Ser cgc Ar g cgc Ar g at c I Ie gac As p 155 gt c ValI acc Thr cca Pr o cca Pr a t ca Ser aac As n gag G31u ct g Le u g aa G31u g ac As p gcg A a 140 gcc A a g ac As p at c I Ie ggt G31y t ct Ser t ca Ser g aa G31u cca Pr a gcc A a t ca Ser 110 aac As n gca A a gcc A a gag G31u at c I Ie 1 90 t ca Ser 15 cgc Ar g ac c Thr g aa G31u tt c Ph e c ac Hi s 95 gca A a c ac Hi s ggc G31y t gc cys g aa G31u 1 75 ggt (31y ag a Ar g cca Pr a at c I Ie gca A a ct c Le u 80 cca Pr a gga G31y aag Ly s t tc Ph e g aa G31u 1 60 g aa G31u aaa Ly s 1 ac a Thr gag G u ggt 0Gy Le u 65 ct c Le u t cg Ser at t I Ie cca Pr a cgc Ar g 145 ccc Pr a t cc Ser ct g Le u gcc A a gga G31y 50 ggc G31y aaa Ly s ct g Le u g ac As p gag G31u 130 cca Pr a ct c Le u ct c Le u at c I Ie g aa G u 35 aac As n ac g Thr at c I Ie g aa 0Gu ct c Le u 115 ct g Le u ct g Le u g ac As p cgc Ar g gct A a 20 gt c ValI gca A a cgt Ar g ct c Le u c ag (31n 1 00 ggc G31y at c Ar g cgc Ar g gca A a 1 g at As p t gg Tr p ct c Le u gt t ValI gca A a gcc A a gca A a gt t ValI aac As n t ac Tyr 1 ct g Le u cga cac gaa ct c at c Arg His G1u Leu Ilie 1 Page 447 Gene Sequence 24- 2- 06. txt gat gcc ctc atc agc aac gcc cac acc tac ctt gag gca agc gat cgt 739 Asp Ala Leu Ile Ser Asn Ala His Thr Tyr Leu G u Ala Ser Asp Arg 200 205 210 gac gag gac atc gca ttc gtg ctc tca cac atc atc gag ctc aac gaa 787 Asp u Asp lie Ala Phe Val Leu Ser His lie lie u Leu Asn u 215 220 225 cag tac ccc ggc gat gtc ggc gtt ctg ggt gct ctg ctg ttg aac ttc 835 G n Tyr Pro y Asp Val y Val Leu Gy Ala Leu Leu Leu Asn Phe 230 235 240 245 tac aaa ctt gcc cca ggc gaa gcc ctc tac ctc gac gcc gca aac ctt 883 Tyr Lys Leu Ala Pro G y G u Ala Leu Tyr Leu Asp Ala Ala Asn Leu 250 255 260 cac gca tac atc agc ggc ctc ggc gta gag atc atg gcg aac tcc gac 931 His Ala Tyr IIe Ser G y Leu G y Val G u IIe IVet Ala Asn Ser Asp 265 270 275 aac gtg ctc cgc ggt gga ctg aca tcc aaa tac gtc gac gtc ccg gag 979 Asn Val Leu Arg G y G y Leu Thr Ser Lys Tyr Val Asp Val Pro G u 280 285 290 ctt gtg cgc gtg ttg gat ttc aac tct ttg gaa aac gct cgc gtg gac 1027 Leu Val Arg Val Leu Asp Phe Asn Ser Leu G u Asn Ala Arg Val Asp 295 300 305 gtt gaa gaa gac ggt gca acg acc cac tac cca gtt cca atc aac gaa 1075 Val G u G u Asp G y Al a Thr Thr His Tyr Pro Val Pro Ile Asn G u 310 315 320 325 ttc caa ctc gat cgc gtt gca gtt cag ggc gaa gca gaa gcc aac cac 1123 Phe G n Leu Asp Arg Val Ala Val G n y G u Ala G u Ala Asn His 330 335 340 gat ggt ccc atg att gtt ctg tgc acc tcc gga act gtt tcc ttg gaa 1171 Asp y Pro IVet Ile Val Leu Cys Thr Ser y Thr Val Ser Leu G u 345 350 355 gca ggg gag aag acc ctc gaa gta gca gca ggt cac gcc gca tgg gtt 1219 Ala G y G u Lys Thr Leu G u Val Ala Ala G y His Ala Ala Trp Val 360 365 370 cca gca aac gac cca acc att gcg atg cgt tct gag gac gca gaa gta 1267 Pro Ala Asn Asp Pro Thr Ile Ala IVet Arg Ser G u Asp Ala G u Val 375 380 385 ttc ctc gct agg gtt tagatctttt tagattaaaa tca 1305 Phe Leu Ala Arg Val 390 <210> 354 <211> 394 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 354 IVet G u Leu Leu u y Ser Leu Arg Thr Tyr Pro Trp Gy Ser Arg 1 5 10 Thr Leu lie Ala Asp Leu Lys Gy uG u Ser Pro Ser Ser Arg Pro 25 G u Ala G u Val Trp Phe G y Ala His Pro Gy Ser Pro Ser Thr lie 40 Page 448 Gene Sequence 24- 2- 06. txt Val Ile Ala Ala Asn Pro Gu Gu Ala G y G y Leu G y Leu Lys Ser Leu Ile Asp Pro G u 130 Arg Pro 145 Pro Leu Ser Leu Arg Hi s G u Al a 210 ie G u 225 Leu Leu Asp Al a [et Al a Val Asp 290 Asn Al a 305 Val Pr o Al a G u Thr Val Hi s Al a 370 Su Asp 385 Asn Ala Leu Asn G u Thr SIle G u Leu 115 Leu Leu Asp Ar g G u 195 Ser Leu Leu Al a Asn 275 Val Ar g SIle Al a Ser 355 Al a Al a Val Al a Al a Al a Val Asn Tyr 165 Leu SIle Ar g G u Phe 245 Leu Asp G u Asp G u 325 Hi s G u Val Val Al a 70 Al a Ar g Pr o Al a Thr 150 Ar g Phe Asp Asp G n 230 Ty r Hi s Asn Leu Val 310 Phe Asp Al a Pr o Phe 390 G u Sy G u Asn Leu 135 Leu Ser Thr Al a G u 215 Tyr Lys Al a Val Val 295 G u G n Sy Sy Al a 375 Leu Al a Al a Sy Ar g 120 Thr Thr bet Thr Leu 200 Asp Pr o Leu Tyr Leu 280 Ar g G u Leu Pr o G u 360 Asn Al a Phe Pr o Phe 105 Asn Su I e Leu Tr p 185 I e I e Gy Al a SIle 265 Ar g Val Asp Asp bet 345 Lys Asp Ar g G u Leu 90 Al a Tyr Phe Phe Thr 170 I le Ser Al a Asp Pr o 250 Ser Sy Leu Sy Ar g 330 I le Thr Pr o Val Asn Ser Ar g Ar g SIle Asp 155 Val Thr Asn Phe Val 235 Sy Sy Sy Asp Al a 315 Val Val Leu Thr G u Leu G u Asp Al a 140 Al a Asp SIle Al a Val 220 Sy G u Leu Leu Phe 300 Thr Al a Leu G u SIle 380 Leu G n Asn Pr o 125 et Leu Asn Pr o Hi s 205 Leu Val Al a Sy Thr 285 Asn Thr Val Cys Val 365 Al a Pr o Al a Ser 110 Asn Al a Al a Su I e 190 Thr Ser Leu Leu Val 270 Ser Ser Hi s G n Thr 350 Al a bet Phe Hi s Al a Hi s Sy Cys G u 175 Sy Tyr Hi s Sy Tyr 255 G u Lys Leu Tyr Sy 335 Ser Al a Ar g <210> 355 Page 449 Gene Sequence 24-2-06.txt <211> 524 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> <223> FRXA01369 <400> 355 aac Asn 1 aac Asn aac Asn t cc Ser ccg Pr o gt g Val aac Asn aac Asn ttg Leu tgg Tr p 145 gaa G u cag G n t ac Tyr cac Hi s aac Asn ctt Leu gtt Val ttc Phe gat Asp 115 gca Al a cca Pr o ttc Phe ctc ggc gat Leu G y Asp 5 ctt gcc cca Leu Ala Pro t ac at c agc Tyr Ile Ser ctc cgc ggt Leu Ar g G y cgc gtg ttg Arg Val Leu 70 gaa gac ggt Su Asp y ctc gat cgc Leu Asp Arg ccc atg att Pro IVet Ile gag aag acc G u Lys Thr 135 aac gac cca Asn Asp Pro 150 gct agg gtt Al a Ar g Val 165 gtc ggc gtt Val G y Val 10 ggc gaa gcc G y G u Al a ggc ctc ggc G y Leu G y 40 gga ctg aca G y Leu Thr gat ttc aac Asp Phe Asn gca acg acc Al a Thr Thr 90 gtt gca gt t Val Al a Val 105 gtt ctg tgc Val Leu Cys 120 ct c gaa gt a Leu G u Val acc att gcg Thr Ile Ala ctg ggt gct Leu G y Al a ctc tac ct c Leu Tyr Leu gt a gag at c Val Gu Ile t cc aaa t ac Ser Lys Tyr tct ttg gaa Ser Leu G u 75 cac tac cca His Tyr Pro cag ggc gaa Gn y u acc tcc gga Thr Ser G y 125 gca gca ggt Al a Al a G y 140 atg cgt tct IVet Arg Ser 155 ct g Leu gac Asp at g et gt c Val aac Asn gtt Val gca Al a 110 act Thr cac Hi s gag G u ct g Leu gcc Al a gcg Al a gac Asp gct Al a cca Pr o gaa G u gtt Val gcc Al a gac Asp ttg Leu gca Al a aac Asn gtc Val cgc Ar g at c SIe gcc Al a t cc Ser gca Al a gca Al a 160 tagatctttt tagat t aaaa tca <210> 356 <211> 167 <212> PRT <213> Corynebacter i um gl <400> 356 Asn G u G n Cys Leu G y 1 5 Asn Phe Tyr Lys Leu Al a Asn Leu His Ala Tyr Ile ut am cum Asp Val y Val Leu Gy Ala Leu Leu Leu 10 Pro Gy G u Ala Leu Tyr Leu Asp Ala Ala 25 Ser y Leu y Val Gu Ile et Ala Asn Page 450 Ser Pr o ValI As n As n Le u Tr p 145 a u As n Le u ValI Ph e As p 115 Al a Pr o Ph e Le u Ar g G31u Le u Pr o G31u As n Al a 1 G31y Le u G31y Ar g I Ie Thr 135 Pr o ValI ene Sequence 24- 2- 06. txt 40 (3 y Leu Thr Ser Lys Tyr Val Asp Phe Asn Ser Leu (31 u Asn Al a Thr Thr Hi s Ty r Pr o Val 90 Val Al a Val O31n 31y G31u Al a 105 110 Val Leu Cys Thr Ser G3 y Thr 120 125 Leu (31u Val Al a Al a G31y Hi s 140 Thr Ilie Al a I'b t Ar g Ser G31u 1 As p Al a Pr o G31u ValI Al a As p <210O> 357 <21 1> 808 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 ODS <222> (808) <223> FRXA01373 <400> 357 ct gcgacgga cct agcaaag gggcgct gac acaagcact g gt cagccacg acct at tcca t tgaagaaaa ggact tgt aa ggC G31y ct c Le u tt c Ph e aac As n gcc Al a gcg Al a ct g Le u ggC G31y gcc Al a gt c ValI gcg Al a gca Al a ac c Thr g aa G31u cca Pr o gca Al a g aa G31u ct a Le u t ac Tyr t ca Ser gga G31y gcg Al a aat As n 75 t ca Ser t gg Tr p t cg Ser cca Pr o 45 ccc Pr o ct t Le u c ag O31n ggt G31y t ct Ser t ca Ser g aa O31u cca Pr o gcc Al a tca aga Ser Ar g 15 cgc cca Ar g Pr o acc at c Thr I Ie gaa gca 03 u Al a t tc ct c Phe Leu 80 cac cca Hi s Pr o 95 Page 451 cgt t tgct gg t gcgcggaca at g gag ct a t tg gaa I'b t 03 u Leu Leu 03 u 1 aca ct g at c gct gat Thr Leu Ilie Al a Asp gag gcc gaa gt c t gg O31u Al a O31u Val Tr p ggt gga aac gca ct C 03 y 03 y Asn Al a Leu t tg ggc acg cgt gt t Leu G31y Thr Ar g Val ct c aaa at c ct c gca Leu Lys Il e Leu Al a t cg ct g gaa cag gcc Ser Leu 03 u 03 n Al a 1 00 Gene Sequence 24-2-06.txt cgt gaa gga ttc gcc cgc gaa aac tca Arg G u G y Phe Ala Arg G u Asn Ser 105 110 ccg aac cgc aac tac cgc gac cca aac Pro Asn Arg Asn Tyr Arg Asp Pro Asn 120 125 gct ctc acg gaa ttc atc gcg atg gca Ala Leu Thr G u Phe Il e Ala IVet Al a 135 140 acc ctc acc att ttc gac gcc ctc gcc Thr Leu Thr Ile Phe Asp Ala Leu Ala 150 155 cgc agc atg ctc acc gtc gac aac gag Arg Ser IVet Leu Thr Val Asp Asn G u 170 ttt acc acc tgg atc acc atc ccc atc Phe Thr Thr Trp Ile Thr Ile Pro Ile 185 190 gat gcc ctc atc agc aac gcc cac acc Asp Al a Leu Ile Ser Asn Al a His Thr 200 205 gac gag gac atc gca ttc gtg ctc tca Asp G u Asp lie Ala Phe Val Leu Ser 215 220 cag tac ccc ggc gat gtc ggc Sn Tyr Pro y Asp Val y 230 235 <210> 358 <211> 236 <212> PRT <213> Corynebacter i um gl ut am cum <400> 358 IVet G u Leu Leu G u G y Ser Leu Arg 1 5 Thr Leu lie Ala Asp Leu Lys Gy u 25 G u Ala G u Val Trp Phe Gy Ala His 40 Gy y Asn Ala Leu Asn u Val lie Leu y Thr Arg Val Al a G u Ala Phe 70 Leu Lys Ile Leu Ala Al a Gy Ala Pro Ser Leu G u G n Ala Arg G u G y Phe 100 105 lie Asp Leu G y Ala Pro Asn Arg Asn 115 120 Pro G u Leu Ile Val Ala Leu Thr G u gca gga att gac ctc ggc gca Al a y Ile Asp Leu y Ala aag Lys ttc Phe gaa G u 160 gaa G u aaa Lys ctt Leu at c SIle cca Pr o cgc Ar g 145 ccc Pr o t cc Ser cga Ar g gag Gu at c SIle 225 gag G u 130 cca Pr o ct c Leu ct c Leu cac Hi s gca Al a 210 gag G u 115 ctg atc gtt Leu Il e Val ctg cgg aac Leu Arg Asn gac cgc tac Asp Arg Tyr 165 cgc gca ctg Arg Ala Leu 180 gaa ct c at c G u Leu Ile 195 agc gat cgt Ser Asp Arg ctc aac gaa Leu Asn G u Thr Tyr I 10 Su Ser I Pro G y Al a Al a Su Asn 75 Leu Ser I 90 Al a Ar g Tyr Arg Phe Ile Page 452 Tr p Ser Pr o Pr o Leu G n Asn Pr o 125 bet Gy Ser Ser G u Pr o Al a Ser 110 Asn Al a Ser Ar g Thr G u Phe Hi s Al a Hi s Gy Ar g Pr o SIe Al a Leu Pr o Gy Lys Phe Gene 130 135 Arg Pro Leu Arg Asn Thr Leu Thr 145 150 Pro Leu Asp Arg Tyr Arg Ser M/et 165 Ser Leu Arg Ala Leu Phe Thr Thr 180 Arg His u Leu Ile Asp Ala Leu 195 200 G u Ala Ser Asp Arg Asp G u Asp 210 215 Ile Gu Leu Asn Gu Gn Tyr Pro 225 230 <210> 359 <211> 1775 <212> DNA <213> Cor ynebact eri um gl ut an cur <220> <221> CDS <222> (1)..(1752) <223> RXA02611 Sequence 24-2-06. t xt 140 Ile Phe Asp Ala Leu Ala Cys u 155 160 Leu Thr Val Asp Asn G u G u u 170 175 Trp Ile Thr Ile Pro Ile Gy Lys 185 190 Ile Ser Asn Ala His Thr Tyr Leu 205 Ile Ala Phe Val Leu Ser His Ile 220 Gy Asp Val Gy 235 <400> 359 gat gcg tgg Asp Al a Tr p 1 aag att gag Lys I I e u cac ggg gct Hi s G y Al a gat agg act Asp Arg Thr gat gt a cgc Asp Val Ar g ctt tta gaa Leu Leu G u ctg cag gtt Leu G n Val gag ctt ttc G u Leu Phe 115 gat Asp 5 ggc Gy ct g Leu ctt Leu cgt Ar g aac Asn gt c Val cgt Ar g cct at g gct acg Pro Met Ala Thr tgg cgt cat gcg att acc act Trp Arg His Ala lie Thr Thr 10 ggt Gy gag G u gac Asp gcc Al a ttg Leu cgt Ar g aca Thr gat Asp gct Al a gcc Al a gcg Al a gag Gu gcc Al a 105 ggt Gy gag G u gcg Al a t cc Ser ct c Leu ttg Leu 90 gct Al a tgg Tr p cag G n ttg Leu gag G u tct Ser acc Thr gt c Val ttg Leu gat Asp gcg Al a aac Asn ttg Leu cgg Ar g agt Ser at g Net aac Asn t cc Ser 125 gag G u ttt Phe aaa Lys ggc Gy act Thr gaa G u tgg Tr p acc Thr gtc Val gtt cat ggc act ttc gct acc act gct Val His G y Thr Phe Al a Thr Thr Al a Page 453 Gene Sequence 24-2-06.txt aag atg ggc ttc gat act gtt Lys Met y Phe Asp Thr Val tac ttc ccg ccg atc cat Tyr Phe Pro Pro Ile His ccg att ggc Pro lie y 160 145 gag G u gat Asp gca Al a gct Al a cag G n 225 acg Thr aag Lys at c SIle gt g Val tgg Tr p 305 ttc Phe aag Lys aac Asn at t SIle tct Ser 385 gcc Al a aac Asn ggt Gy cat Hi s 195 gca Al a gcc Al a ttg Leu cag G n gaa G u 275 aca Thr tgg Tr p gcg Al a ggt Sy gag G u 355 cgt Ar g cag G n acg Thr aag Lys 165 ccg Pr o cgg Ar g gaa G u gat Asp gat Asp 245 at t SIle gt c Val cgc Ar g at t SIle gcg Al a 325 t cc Ser acc Thr aac Asn ggt Gy tct Ser 405 150 ggc Gy tgg Tr p tt g Leu ct c Leu cat Hi s 230 ggc Gy t at Tyr t at Tyr gt g Val tct Ser 310 tct Ser cag G n gag G u ct g Leu gga G y 390 cct Pr o cgc Ar g gct Al a ggc G y aat Asn 215 cca Pr o acc Thr ccc Pr o cgt Ar g gat Asp 295 gcc Al a act Thr tct Ser ttc Phe ttt Phe 375 cgc Ar g gt g Val aat Asn at t l Ie acc Thr 200 ttg Leu tgg Tr p at t SIle at c SIle gt g Val 280 aac Asn at c l Ie cgc Ar g t ac Tyr gct Al a 360 gt g Val gcc Al a tgg Tr p aat Asn ggt G y 185 at t SIle gaa G u gcg Al a gcg Al a aac Asn 265 gt g Val ccg Pr o cat Hi s ccg Pr o acc Thr 345 act Thr aac Asn at g Net ggc G y acg Thr 170 tct Ser gaa G u gtt Val cag G n t at Tyr 250 ttt Phe aag Lys cac Hi s aaa Lys gca Al a 330 t ac Tyr gag G u act Thr ttc Phe gt a Val 410 155 ct g Leu aaa Lys gat Asp gca Al a gaa G u 235 gca Al a gat Asp ttc Phe act Thr t ca Ser 315 cgt Ar g ttc Phe at c SIle ccc Pr o gct Al a 395 t at Tyr acc Thr gat Asp ttc Phe ct c Leu 220 cac Hi s gaa Gu aat Asn tgg Tr p aag Lys 300 aac Asn ct g Leu acc Thr gcc Al a gac Asp 380 at c l Ie t cc Ser ccg Pr o ggt G y cag G n 205 gat Asp cgc Ar g aac Asn gat Asp gt g Val 285 ccc Pr o cct Pr o t at Tyr tgg Tr p ccc Pr o 365 at t SIle cgc Ar g gga G y gaa G u ggg G y 190 gcg Al a ct a Leu gag G u cca Pr o gct Al a 270 gat Asp gct Al a gag G u ttc Phe aag Lys 350 at g tet ttg Leu gcc Al a t at Tyr cct Pr o 175 cat Hi s ttg Leu gct Al a ttt Phe ccg Pr o 255 ccg Pr o ttg Leu aat Asn gt c Val ttg Leu 335 gt c Val gcg Al a cat Hi s gca Al a gag 15u 415 cat Hi s gat Asp ttg Leu ct g Leu ttc Phe 240 aag Lys aag Lys ggt Sy ttc Phe at t SIe 320 t cc Ser acc Thr gat Asp gcg Al a ttg Leu 400 ct c Leu 480 528 576 624 672 720 768 816 864 912 960 1008 1056 1104 1152 1200 1248 Page 454 Gene Sequence 24-2-06.txt ttt gag cac gag gcc gtc aag cct Phe G u His G u Ala Val Lys Pro ggt gag G u ggc Gy gcg Al a 465 gac Asp aat Asn gct Al a cag G n gag G u 545 ttt Phe cgc Ar g t ac Ty r 435 tct Ser cct Pr o gat Asp gt g Val gtt Val 515 gag G u aac Asn ctt Leu at c SIle ct g Leu gag Gu ttg Leu at c SIle 485 at t SIle ctt Leu cgc Ar g gt c Val gaa G u 565 ccc Pr o t ac Tyr 455 caa G n gcc Al a gt c Val ctt Leu gcg Al a 535 ct c Leu cca Pr o cgc Ar g 440 at c SIle ct a Leu t ac Tyr aac Asn gga Gy 520 at c l Ie gag G u gcg Al a Sy 425 gat Asp gct Al a cgc Ar g t cc Ser ttg Leu 505 gcg Al a acc Thr ccc Pr o tct Ser ttc Phe ct g Leu aac Asn aag Lys 490 gat Asp ctt Leu ggc Gy ct a Leu cgc Ar g 570 gag G u ct c Leu at c SIle 475 gtt Val cca Pr o ggc Gy t cc Ser cgc Ar g 555 cgt Ar g ggt Gy aac Asn 460 cac Hi s gat Asp cgt Ar g tta Leu cgt Ar g 540 gat Asp gag G u gct Al a 445 cag G n ttc Phe gct Al a agt Ser gaa Gu 525 t ac Tyr gt c Val cgt Ar g ct g Leu at c SIle cac Hi s ttg Leu gct Al a 510 gcg Al a ct g Leu gcc Al a ct c Leu tcg gaa gag tac ttg gat tct Ser G u G u Tyr Leu Asp Ser 430 gaa G u cgt Ar g gaa G u acc Thr 495 cgt Ar g ggt Gy tgg Tr p cac Hi s gcg Al a 575 cgt Ar g cgc Ar g gcg Al a 480 gga Gy gag G u gca Al a t ca Ser at c SIe 560 tgg Tr p 1296 1344 1392 1440 1488 1536 1584 1632 1680 1728 1775 acc tac cgc gcg Thr Tyr Arg Ala taatttccca tctctgtacc ttc <210> 360 <211> 584 <212> PRT <213> Corynebact <400> 360 Asp Ala Trp Ser 1 Lys i e G u Ala Hi s G y Al a G n Asp Arg Thr Ala Asp Val Arg Ala Leu Leu G u Leu er i um gl ut am cum Asp Pro IVet Ala Thr 5 G y n G y Ser Asp Leu Phe G u Arg Ala 40 Leu Phe Asp Val Al a 55 Arg Leu Ala Pro Ala 70 Asn Pro Leu Arg G u Tr p Ar g Hi s 10 G u Leu Tyr Al a G u Asn Ser Ser Leu Leu Thr Al a Leu Val Thr 90 Page 455 Al a Asn Leu Ar g Ser Net Thr Phe Lys Gy Thr Su Gene Sequence 24-2-06.txt Leu G u Val Lys 145 G u Asp Al a Al a G n 225 Thr Lys I le Val Tr p 305 Phe Lys Asn I le Ser 385 Al a Phe G u Val Phe 115 Gy Gy Asn Gy Hi s 195 Al a Al a Leu G n G u 275 Thr Tr p Al a Gy G u 355 Ar g G n Thr Hi s Tyr 435 Val u Arg Arg Ala Ala Leu Val Asn Ser Trp Tyr 110 Ar g Phe Asp Lys 165 Pr o Ar g G u Asp Asp 245 I le Val Ar g SIle Al a 325 Ser Thr Asn Gy Ser 405 Al a Leu Ser Al a Thr 150 Gy Tr p Leu Leu Hi s 230 Gy Ty r Ty r Val Ser 310 Ser G n G u Leu Gy 390 Pr o Val Ar g Thr Thr 135 Val Ar g Al a Gy Asn 215 Pr o Thr Pr o Ar g Asp 295 Al a Thr Ser Phe Phe 375 Ar g Val Lys Pr o Gy 120 Thr Tyr Asn l Ie Thr 200 Leu Tr p I e I e Val 280 Asn l Ie Ar g Tyr Al a 360 Val Al a Tr p Pr o Ar g 440 105 Gy Al a Phe Asn Gy 185 I le G u Al a Al a Asn 265 Val Pr o Hi s Pr o Thr 345 Thr Asn let Gy Gy 425 Asp Asp Al a Pr o 155 Leu Lys Asp Al a G u 235 Al a Asp Phe Thr Ser 315 Ar g Phe SIle Pr o Al a 395 Tyr Su Su Ser 125 G u Hi s Pr o Gy G n 205 Asp Ar g Asn Asp Val 285 Pr o Pr o Tyr Tr p Pr o 365 I le Ar g Gy Tyr Al a 445 Gy Ar g Pr o G u Gy 190 Al a Leu G u Pr o Al a 270 Asp Al a G u Phe Lys 350 let Leu Al a Tyr Leu 430 Leu Thr Val SIle Pr o 175 Hi s Leu Al a Phe Pr o 255 Pr o Leu Asn Val Leu 335 Val Al a Hi s Al a G u 415 Asp G u Pr o Al a Gy 160 Hi s Asp Leu Leu Phe 240 Lys Lys Gy Phe SIe 320 Ser Thr Asp Al a Leu 400 Leu Ser Ar g Gy Asp Ser Leu u Asp Tyr Ile Ala Leu Leu Asn n lie Arg Arg Page 456 Al a 465 Asp Asn Al a G n G u 545 Phe Ar g Pr o Asp Val Val 515 G u Asn Leu SIle Leu I le 485 I le Leu Ar g Val G u 565 Thr 455 G n Al a Val Leu Al a 535 Leu Pr o Ar g Gene Sequence 24- 2- 06. t xt 460 Leu Arg Asn lie His Phe His 475 Tyr Ser Lys Val Asp Al a Leu 490 Asn Leu Asp Pro Arg Ser Ala 505 510 G y Al a Leu G y Leu G u Al a 520 525 l e Thr y Ser Arg Tyr Leu 540 G u Pro Leu Arg Asp Val Ala 555 Ala Ser Arg Arg G u Arg Leu 570 Al a G u Thr 495 Ar g Gy Tr p Hi s Al a 575 Al a 480 Gy G u Al a Ser SIe 560 Tr p <210> 361 <211> 2316 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(2293) <223> RXA02612 <400> 361 gaacttccag cgtctcgccg tgagcgtctc gcg taatttccca tctctgtacc ttctatcaag gat gcg Al a tgc Cys gct Al a ttg Leu at t SIle gaa G u cac Hi s cat Hi s tcg Ser at c SIle gca Al a acc Thr acc Thr gat Asp at c SIle gac Asp gac Asp cct Pr o at c SIle cct Pr o cgc Ar g acc Thr tta Leu 75 gat Asp cct Pr o cat Hi s acg Thr t cc Ser ggt Gy caa G n gaa G u gga Gy cgc Ar g 45 cac Hi s cac Hi s gaa G u gca Al a ttt Phe cag G n gt c Val cgc Ar g ccg Pr o Itggcgcg aaatcaagac ctaccgcgcg tatcatc atg acc gtt gac ccc IVet Thr Val Asp Pro 1 gat ctg gcc cgc ctg cgc cac Asp Leu Ala Arg Leu Arg His 15 tat ggt tgg cat gag acc gaa Tyr G y Trp His G u Thr G u gtc ggc gcg acg cag gtt aat Val y Ala Thr G n Val Asn atg acc cct atc ggc gac gac IVet Thr Pro lie y Asp Asp gag cgc gct gac tat cgc ttg G u Arg Ala Asp Tyr Arg Leu 80 cag gtc aag gct gat cca tac G n Val Lys Ala Asp Pro Tyr 95 100 Page 457 Gene Sequence 24-2-06.txt tac ttc ctc ccc acc gta ggc gag atg gat Tyr Phe Leu Pro Thr Val Gy u lMt Asp att tac ctc ttc tct gag Ile Tyr Leu Phe Ser G u 115 gga Gy t ac Tyr gct Al a 150 aat Asn gag G u gt c Val cgt Ar g gag G u 230 gat Asp tgg Tr p t ac Tyr gag G u gca Al a 310 gac Asp gcc Al a gcc Al a cat Hi s 120 act Thr aac Asn t cc Ser ttc Phe acc Thr 200 gca Al a cag G n gca Al a tgg Tr p gca Al a 280 ccc Pr o acc Thr ttc Phe ttc Phe t at Tyr 360 cgt Ar g ct c Leu at t SIle cat Hi s 170 cca Pr o gaa Gu ct g Leu cag G n aag Lys 250 aag Lys ctt Leu ggt Sy cgt Ar g gcc Al a 330 aag Lys cac Hi s tt g Leu gga G y ggc G y 155 ccg Pr o ggc G y ggc Gy gcg Al a gat Asp 235 cca Pr o aac Asn ggc Sy ggt G y tgg Tr p 315 cgc Ar g gat Asp cct Pr o tgg Tr p aca Thr 140 tgc Cys at g let at a SIle caa G n ccg Pr o 220 t cc Ser at g et t at Tyr t ac Tyr t cc Ser 300 ggt G y ggt G y gat Asp gac Asp gag G u 125 gtt Val gca Al a cgt Ar g gag G u cgt Ar g 205 gca Al a gag G u agt Ser gag G u acc Thr 285 tgg Tr p act Thr at t SIle tgg Tr p tgg Tr p 365 110 at t SIle cgt Ar g gt g Val tct Ser gaa G u 190 cgt Ar g acc Thr tgg Tr p gt c Val gat Asp 270 cac Hi s ggt G y cca Pr o ggc G y gct Al a 350 agg Ar g ggt Gy acc Thr ggt G y 160 ggt G y gaa G u aag Lys tct Ser cgc Ar g 240 gag G u gct Al a gaa G u cag G n cag G n 320 at c SIle gcc Al a ggc Gy gcc Al a gca Al a 145 ggc G y ggt G y gt g Val gcc Al a at t SIle 225 gag G u gt c Val act Thr ttc Phe gt c Val 305 ttc Phe at g et cgc Ar g gaa G u aac Asn 130 ttt Phe ttc Phe tcg Ser t ac Tyr gat Asp 210 gt c Val cgt Ar g cac Hi s gag G u ct c Leu 290 acc Thr cgt Ar g gac Asp ttt Phe caa G n 370 aag Lys gt g Val ggt G y ct g Leu 180 ttc Phe at g bet t cc Ser caa G n ggt Sy 260 gtt Val gt c Val t ac Tyr ct a Leu gtt Val 340 ggc G y gat Asp acc Thr tgg Tr p tgg Tr p 165 tgg Tr p gcc Al a gct Al a tct Ser act Thr 245 tct Ser gat Asp gca Al a t ac Tyr gt c Val 325 cct Pr o gaa G u tgg Tr p 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 Page 458 Gene Sequence 24-2-06.txt ggc acc ctg gtc ttt gac ttc ggt Sy Thr Leu Val Phe Asp Phe y cgc aac gaa gtc Arg Asn G u Val cgc aac ttc ctc Arg Asn Phe Leu gt c Val 390 cgc Ar g cac Hi s gca Al a cct Pr o acc Thr 470 at g Met cac Hi s ttc Phe ggc Gy gcc Al a 550 aag Lys gct Al a gag G u t ac Tyr ttc Phe 630 aat Asn gac Asp gaa G u cag G n 440 gcg Al a cca Pr o tgg Tr p gca Al a gag G u 520 ggt Gy ggt Gy ct g Leu ggc Gy cac Hi s 600 gac Asp tgg Tr p ttg Leu gt g Val 410 gaa Gu ct g Leu acc Thr tgg Tr p cac Hi s 490 cac Hi s ttt Phe ct g Leu cgc Ar g ttc Phe 570 gga Gy gcc Al a cct Pr o aag Lys tac Ty r 395 gcc Al a cca Pr o cag G n at c SIle gac Asp 475 gac Asp cac Hi s gt a Val tgg Tr p acc Thr 555 at g bet ct g Leu at c l Ie gcg Al a ggc G3y 635 380 tgg Tr p t cc Ser aac Asn gaa G u gct Al a 460 ggc Gy acc Thr agt Ser ctt Leu gac Asp 540 ttc Phe ggt Gy cca Pr o cgc Ar g ct g Leu 620 gac Asp gaa G u ct g Leu t ac Tyr 430 aac Asn gaa G u ttg Leu gag G u ct c Leu 510 at c SIle at g et gcg Al a gag G u gat Asp 590 ct g Leu act Thr gcc Al a gaa G u t ac Tyr 415 ggt Gy gcc Al a t ca Ser gga Gy t ac Tyr 495 act Thr tct Ser cct Pr o t ac Tyr ttt Phe 575 at t SIle acc Thr cag G n acc Thr ttc Phe 400 ct c Leu ggt Gy acg Thr act Thr ttc Phe 480 ttc Phe ttc Phe cac Hi s ggc Gy at g bet 560 ggt Gy gt c Val cgc Ar g gat Asp aac Asn 640 at c SIle t ac Tyr gag G u ct g Leu 450 tgg Tr p ct c Leu aaa Lys ttg Leu gaa G u 530 acg Thr t ca Ser cgt Ar g ggc Gy ct c Leu 610 acc Thr at t SIle gat Asp t cc Ser aac Asn 435 cga Ar g cct Pr o aag Lys aac Asn gt g Val 515 gt c Val tgg Tr p cac Hi s gaa G u tgg Tr p 595 aac Asn gga Gy ttg Leu ggt Gy cgt Ar g 420 ct c Leu ct g Leu ggc Gy tgg Tr p cct Pr o 500 t ac Tyr gt c Val aac Asn cca Pr o gag G u 580 caa G n ggt Gy gaa G u gcg Al a 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 Page 459 act Thr tct Ser gaa G u gaa G u aat Asn 710 ttc Phe ttc Phe acc Thr aag Lys 680 gat Asp gcc Al a t ca Ser agc Ser 650 cag G n gtt Val gct Al a t ca Ser cag G n 730 Gene Sequence 24-2-06.txt ggc tcc cag atg ctg tgt gta ttc Sy Ser G n Met Leu Cys Val Phe 655 gag tac cag ctc ggt gtt gca gcg G u Tyr G n Leu G y Val Al a Al a 670 675 aac act gat gat gct gaa ttc ctc Asn Thr Asp Asp Ala G u Phe Leu 685 690 tcc gtt caa gca gca gcg aca cca Ser Val G n Ala Ala Ala Thr Pro 700 705 tca ctg cat gtc cca gcg atg agt Ser Leu His Val Pro Ala et Ser 720 taggacacag gaaaatgcat cct ct g Leu ggc Sy gca Al a gat Asp cag G n 725 2083 2131 2179 2227 2275 2316 <210> 362 <211> 731 <212> PRT <213> Corynebact <400> 362 IVet Thr Val Asp 1 Ala Arg Leu Arg Trp His u Thr Ala Thr G n Val Pro lie y Asp Ala Asp Tyr Arg Lys Ala Asp Pro 100 Tyr Leu Phe Ser 115 Ala Asn i e Lys 130 Ala Phe Thr Val 145 G y Phe Asn G y G y Ser G y Leu 180 er i um gl ut ai cum Pro Ala Ser His Ile 5 His Cys Asn His His 25 Su Al a y Ser Val 40 Asn Leu Leu Ile Asp Asp Ile Phe Ala lle 70 Leu G u Val Thr Trp Tyr Tyr Phe Leu Pro 105 G u G y Arg His G u 120 Thr Tyr G n Thr Al a 135 Trp Ala Pro Asn Ala 150 Trp Asn Ala Ser G n 165 Trp G u Leu Phe Ile 185 SIle Pr o Ar g Thr Leu Asp Val Leu Gy Gy 155 Pr o Gy G u Gy Ar g Hi s Hi s G u G u G u 125 Val Al a Ar g G u Al a Phe G n Val Ar g Pr o et 110 SIle Ar g Val Ser G u 190 Asp Tyr Val bet G u G n Asp Leu Sy Val bet 175 Sy Val Tyr Lys Phe Ala Val G n Thr Arg u Gy n Arg Arg Asp Lys Page 460 Gene Sequence 24-2-06.txt 200 Ala G u Leu Ala Al a SIle 225 G u Val Thr Phe Val 305 Phe let Ar g G u Val 385 Hi s Asp Ar g Val Ser 465 Ser Ser Ser Asp Asp 545 Pr o Al a Ser Leu Leu 275 Pr o Gy Al a Tr p Asp 355 Lys Asn Asp Ser Asn 435 Ar g Pr o Lys Asn Val 515 Val Al a Ser Thr 245 Ser Asp Al a Tyr Val 325 Pr o G u Tr p Leu Leu 405 G u G u Hi s Val Asn 485 Val Al a Hi s Ar g G u 230 Asp Tr p Ty r G u Al a 310 Asp Al a Al a Gy Val 390 Ar g Hi s Al a Pr o Thr 470 bet Hi s Phe Gy Ar g 215 Ty r Leu Ar g Val Hi s 295 Pr o Al a Hi s Leu Thr 375 Al a Val Gy Val Gy 455 Al a Gy Ar g Ser Lys 535 n Al a Tr p Al a 280 Pr o Thr Phe Phe Tyr 360 Leu Asn Asp G u G n 440 Al a Pr o Tr p Al a G u 520 Gy Tr p Ser Gy 265 Asp Phe Ser Hi s Pr o 345 G u Val Al a Al a Tr p 425 Phe Leu Thr let Phe 505 Ar g Ser Asp 235 Pr o Asn Gy Gy Tr p 315 Ar g Asp Pr o Asp Tyr 395 Al a Pr o G n SIle Asp 475 Asp Hi s Val Tr p Pr o 220 Ser let Tyr Tyr Ser 300 Gy Gy Asp Asp Phe 380 Tr p Ser Asn G u Al a 460 Gy Thr Ser Leu Asp 540 205 Al a Thr G y Ser u Ser G u Thr 285 Tr p Thr SIle Tr p Tr p 365 Gy SIle let SIle let 445 G u Gy Leu G u Pr o 525 Ar g Leu Tyr 255 Leu Val Tyr Asp Val 335 Leu Ar g Asn G u Tyr 415 Gy Al a Ser Gy Tyr 495 Thr Ser Pr o Thr Trp Asn Lys Al a Ala y Leu Arg Thr Phe Leu Ala Tyr IVet 550 555 560 Page 461 Trp Ser His Pro G n Asp Ser Phe 625 Asn Cys Val G u Al a 705 Al a G u Tr p 595 Asn Gy Leu Phe Al a 675 Leu Pr o Ser Gy 565 Tr p Gy Val Gy Phe 645 Leu Gy Al a Asp G n 725 Gene Sequence 24-2-06.txt Lys Lys Leu Leu Phe Met G y n G u Phe G y 570 575 Al a G u Ty r Phe 630 Thr Ser G u G u Asn 710 Phe G u Tyr Ser 615 Thr Ar g Sy Tr p Asn 695 Phe Tyr Sy Hi s 600 Asp Tr p Phe Thr Lys 680 Asp Al a Ser G n 585 G u Ser Asn Sy Ser 665 Leu SIle Tyr Leu Sy Al a Pr o Lys Ser 650 G n Val Al a Ser G n 730 Leu l Ie Al a Sy 635 Asp Pr o Leu Thr Leu 715 Lys <210> 363 <211> 1913 <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (1)..(1890) <223> RXN01884 <400> 363 gtg act gcc att gaa ttg atg ccg gtc Val Thr Ala lie G u Leu Met Pro Val 1 5 cgt ctc cgc gac cta gga atg cgc aac Arg Leu Arg Asp Leu G y Iet Arg Asn 25 ggc ttc ttt gcg ccc tac aac gat tac Sy Phe Phe Ala Pro Tyr Asn Asp Tyr 40 ggc gcc gtg gcc gag ttc aaa ggc ttg Sy Ala Val Ala G u Phe Lys y Leu ggc ctc gaa gta att ttg gat gtg gtc Sy Leu G u Val lie Leu Asp Val Val 70 aac cac atg ggc ccc acc atc gcg ttc Asn His \et G y Pro Thr lie Ala Phe Tr p Thr 605 Hi s Asp Ser Tyr Thr 685 Val Leu ct g Leu t ac Tyr aag Lys t ac Tyr acc Thr gac Asp Asp 590 Leu Thr Al a G n G n 670 Asp G n Hi s cag G n aac Asn aac Asn cac Hi s gcc Al a aac Asn SIle Thr G n Thr vet 655 Leu Asp Al a Val gat Asp tct Ser ccc Pr o gaa G u gag G u gag G u Val Ar g Asp Asn 640 Leu Sy Al a Al a Pr o 720 cac cag 1 Hi s n I 10 tac tgg Tyr Trp gct gcc Al a Al a gtg cgc Val Arg t ac aac Tyr Asn I 75 cga ggc Arg Gy I 90 Page 462 gat Asp ttc Phe ggt G y gcg Al a ggc G y gcg Al a Gene Sequence 24-2-06.txt t ac Tyr ggt Sy at t SIle ttc Phe 145 gac Asp t cc Ser t ac Tyr t ac Tyr ggt Gy 225 aac Asn ggc Gy gcc Al a aac Asn cgt Ar g 305 ggc Sy ggc G y cga Ar g ggt Sy 115 gat Asp ttc Phe ct g Leu gt c Val gt g Val 195 gac Asp ttc Phe cgt Ar g acc Thr ggt Sy 275 ggc G y cag G n cct Pr o aac Asn gtt Val t cc Ser ct g Leu ctt Leu acc Thr 165 ct c Leu aac Asn gt c Val t cc Ser ccc Pr o 245 aat Asn gac Asp gaa G u cga Ar g ttg Leu 325 gt c Val gag G u ct c Leu cgc Ar g gcc Al a 150 tt c Phe at t SIle tt c Phe cgt Ar g cga Ar g 230 act Thr gac Asp ggt G y gga G y cgc Ar g 310 t cc Ser tac Ty r ggc Gy aat Asn t ac Tyr 135 tct Ser ttc Phe gct Al a cca Pr o gat Asp 215 ct a Leu gca Al a ttg Leu cgg Ar g cca Pr o 295 aac Asn cac Hi s tgc Cys gat Asp gt c Val 120 tgg Tr p acc Thr gac Asp gag G u cca Pr o 200 ttc Phe act Thr tcg Ser gt c Val gac Asp 280 act Thr ttc Phe ggt G y caa G n cgc cgt cat Ar g 105 cgc Ar g gt c Val ctt Leu ct g Leu ccg Pr o 185 ct g Leu tgg Tr p ggt Gy at c SIle agt Ser 265 ggt G y gac Asp ct c Leu gat Asp gac Asp 345 Arg Hi s gac Asp acc Thr gct Al a gt c Val 170 tgg Tr p tgg Tr p cgt Ar g t cc Ser aac Asn 250 t ac Tyr gaa G u gat Asp acc Thr gaa G u 330 aat Asn ttg Leu ccg Pr o gag G u cgt Ar g 155 caa G n gat Asp act Thr ggt Gy tct Ser 235 ttt Phe aac Asn t ca Ser cct Pr o acc Thr 315 at g et gaa G u gt g Val t ac at g gac Tyr IVet Asp 110 cac t ca ct g Hi s Ser Leu 125 atg cat gt c et His Val 140 gaa ttt gat G u Phe Asp caa gac ccg Sn Asp Pro gtt ggc gaa Val y u 190 gag tgg aac Su Trp Asn 205 gag cca gca G u Pro Ala 220 gat ttg tat Asp Leu Tyr gtg act gct Val Thr Al a gag aag cac G u Lys Hi s 270 cac aac cgt His Asn Ar g 285 gag at t at g G u I le IVet 300 ttg ttg ctg Leu Leu Leu gcc cgt acc Al a Arg Thr ctg gcg tgg Leu Al a Trp 350 agc ttc acc Ser Phe Thr 365 t ac Tyr cag G n gac Asp gat Asp gt g Val 175 ggc G y ggt Gy acc Thr gca Al a cac Hi s 255 aac Asn t cc Ser cag G n t cc Ser caa G n 335 gt g Val agg Ar g acc Thr ct g Leu ggc G y gtt Val 160 gt c Val gga G y aaa Lys ttg Leu aac Asn 240 gac Asp at g Net tgg Tr p ct g Leu cag G n 320 aac Asn aat Asn cgt Ar g 336 384 432 480 528 576 624 672 720 768 816 864 912 960 1008 1056 1104 tgg gat cag gct gaa gaa aac gct gac Trp Asp n Ala G u G u Asn Ala Asp 355 360 Page 463 Gene Sequence 24-2-06.txt ttg Leu ctt Leu 385 tgg Tr p gct Al a gag G u at g let cat Hi s 465 ggc Gy gtt Val t ac Tyr ctt Leu gca Al a 545 gaa Gu gat Asp gat Asp act Thr gcg Al a 625 cgt Ar g ggt Sy gt a Val ggt Sy gat Asp 435 aac Asn ggt Gy ccg Pr o gcc Al a aag Lys 515 gca Al a aag Lys gct Al a at t SIle gt a Val 595 tct Ser cga Ar g cct Pr o aat Asn 405 t ca Ser cga Ar g cac Hi s aag Lys gag Gu 485 t cc Ser gag Gu aag Lys gct Al a act Thr 565 gat Asp gca Al a t cc Ser gca aac Al a Asn 375 ttg ggc Leu G y 390 gga acc G y Thr ctg cag Leu G n gga cag G y G n ttc gaa Phe G u 455 tgg aag Trp Lys 470 gat ct c Asp Leu acg atg Thr lbet gaa aag G u Lys gaa gct G u Al a 535 gaa gat Su Asp 550 cag gaa G n u gag gt a G u Val gag gt c G u Val gag cag G u G n 615 cac cca gt a Hi s Pro Val gcc Al a ttg Leu gt g Val aaa Lys 440 cct Pr o ctt Leu acc Thr ct g Leu at c SIle 520 gct Al a gct Al a gct Al a gcg Al a gag G u 600 gct Al a gat Asp at g let ttc Phe 425 at c SIle at c SIle ttg Leu at c SIle ct g Leu 505 gct Al a gag G u gct Al a gaa G u gaa G u 585 act Thr gag G u ttt agg cgc agg Phe Arg Arg Arg 380 cgt gac cgc gat Arg Asp Arg Asp 395 caa gat gac tgg G n Asp Asp Trp aac ggc gat gcc Asn G y Asp Al a 430 gat gac tcc ttc Asp Asp Ser Phe 445 ttc aat ctc cct Phe Asn Leu Pro 460 gat acc acc gaa Asp Thr Thr G u 475 gct ggc gga acc Al a G y y Thr cag gtg gag gct Sn Val u Ala 510 gaa aag cgt gag G u Lys Arg G u 525 cgc gaa ttg gaa Arg G u Leu G u 540 gct ctc cac ctt Ala Leu His Leu 555 gcc cat caa cac Ala His G n Hi s cca caa gag ct g Pro G nG u Leu 590 ccc gac acc gag Pro Asp Thr G u 605 gct tca gag gag Al a Ser G u G u 620 cag ttc G n Phe at c SIle gac Asp 415 at c l Ie at c SIle cct Pr o gcg Al a at c SIle 495 ccg Pr o caa G n ct g Leu gcg Al a ggt G y 575 cca Pr o cct Pr o cct Pr o gca Al a 400 ttc Phe gaa G u ttg Leu gag G u gt g Val 480 act Thr gac Asp gaa G u gcg Al a gca Al a 560 gct Al a caa G n gac Asp gaa G u 1152 1200 1248 1296 1344 1392 1440 1488 1536 1584 1632 1680 1728 1776 1824 1872 gac gaa gaa gag Asp G u G u Gu tagtacaccg aaagtggcgt cgc 1913 Page 464 Gene Sequence 24-2-06.txt <210> 364 <211> 630 <212> PRT <213> Corynebacteri um gl ut arr cum <400> 364 Val Thr Ala lle G 1 Arg Leu Arg Asp Le G y Phe Phe Al a Pr Sy Ala Val Ala G Sy Leu u Val II Asn His Net G y Pr 8 Tyr Tyr Arg Leu Va 100 Sy Thr y Asn Se 115 Ile IVet Asp Ser Le 130 Phe Arg Phe Asp Le 145 Asp Arg Leu Ala Th 16 Ser G n Val Lys Le 180 Tyr n Val y As 195 Tyr Arg Asp Thr Va 210 G y G u Phe Al a Se 225 Asn y Arg Arg Pr 24 Sy Phe Thr Leu As 260 Ala Asn G y u As 275 Asn Cys Gy Val G 290 Ar a Ala G n G n Ar u Leu 5 u G y o Tyr u Phe e Leu 70 o Thr 5 l G u r Leu u Arg u Al a 150 ir Phe ;5 u I e n Phe I Arg r Arg 230 o Thr .5 n Asp p Gy uGy g Arg 310 Net et Asn Lys Asp SIle Sy Asn Tyr 135 Ser Phe Al a Pr o Asp 215 Leu Al a Leu Ar g Pr o 295 Asn Val Asn 25 Tyr Leu Val Phe Ar g 105 Ar g Val Leu Leu Pr o 185 Leu Tr p Gy l e Ser 265 Sy Asp Leu Hi s 10 Tyr Al a Val Tyr Ar g 90 Ar g Asp Thr Al a Val 170 Tr p Tr p Ar g Ser Asn 250 Tyr a u Asp G n Tr p Al a Ar g Asn Sy Hi s Pr o G u Ar g 155 G n Asp Thr Sy Ser 235 Phe Asn Ser Pr o Leu Tyr Lys Tyr Thr Asp bet Ser 125 Hi s Phe Asp Sy Tr p 205 Pr o Leu Thr Lys Asn 285 I e G n Asn Asn Hi s Al a Asn Asp 110 Leu Val Asp Pr o G u 190 Asn Al a Tyr Al a Hi s 270 Ar g bet Asp Ser Pr o G u G u G u Tyr a n Asp Asp Val 175 Sy Sy Thr Al a Hi s 255 Asn Ser Gn 305 Thr Thr Leu Leu Leu Ser G n 315 320 Page 465 Sy Thr Pro lMt Leu Ser 325 Gene Sequence 24-2-06.txt His y Asp G u let Ala Arg Thr Sn Asn 335 Sy Tr p Leu Leu 385 Tr p Al a G u let Hi s 465 Sy Val Tyr Leu Al a 545 G u Asp Asp Thr Al a 625 Asn G n 355 Ar g Sy Val Sy Asp 435 Asn Sy Pr o Al a Lys 515 Al a Lys Al a SIle Val 595 Ser G u Val G u Ar g Pr o Asn 405 Ser Ar g Hi s Lys G u 485 Ser G u Lys Al a Thr 565 Asp Al a Ser G u Cys Asn Asn 375 Sy Thr G n G n G u 455 Lys Leu let Lys Al a 535 Asp G u Val Val G n 615 G n Al a 360 Hi s Al a Leu Val Lys 440 Pr o Leu Thr Leu SIle 520 Al a Al a Al a Al a G u 600 Al a Asp 345 Asp Pr o Asp let Phe 425 I le SIle Leu SIle Leu 505 Al a G u Al a G u G u 585 Thr G u 330 Asn Leu Val Val Thr 410 Phe Hi s Asp Val G u 490 Ar g Al a Lys G u Leu 570 G u G u Val G u Val Phe Ar g 395 G n Asn Asp Phe Asp 475 Al a G n G u Ar g Al a 555 Al a Pr o Pr o Al a Al a Phe 365 Ar g Ar g Asp Asp Ser 445 Leu Thr Sy G u Ar g 525 Leu Hi s G n G u Thr 605 G u Val Ar g G n SIe Asp 415 I e I e Pr o Al a SIe 495 Pr o G n Leu Al a Sy 575 Pr o Pr o Pr o <210> 365 <211> 1496 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS Page 466 Gene Sequence 24-2-06. t xt <222> (1)..(1473) <223> FRXA01884 <400> 365 atg cat gtc gac ggc ttc cgc ttc gac ctt gcc tct acc ctt gct cgt 48 Nbt His Val Asp 3 y Phe Arg Phe Asp Leu Ala Ser Thr Leu Ala Arg 1 5 10 gaa ttt gat gat gtt gac cgc ctg gca acc ttc ttc gac ctg gtc caa 96 Su Phe Asp Asp Val Asp Arg Leu Ala Thr Phe Phe Asp Leu Val n 25 caa gac ccg gtg gtc tcc cag gtc aag ctc att gct gag ccg tgg gat 144 Sn Asp Pro Val Val Ser n Val Lys Leu Ile Ala u Pro Trp Asp 40 gtt ggc gaa ggc gga tac caa gtg ggt aac ttc cca cca ctg tgg act 192 Val y u y y Tyr n Val y Asn Phe Pro Pro Leu Trp Thr 55 gag tgg aac ggt aaa tac cgc gac act gtc cgt gat ttc tgg cgt ggt 240 Su Trp Asn G y Lys Tyr Arg Asp Thr Val Arg Asp Phe Trp Arg G y 70 75 gag cca gca acc ttg ggt gaa ttc gct tcc cga cta act ggt tcc tct 288 G u Pro Ala Thr Leu G y G u Phe Ala Ser Arg Leu Thr G y Ser Ser 90 gat ttg tat gca aac aac ggc cgt cgc ccc act gca tcg atc aac ttt 336 Asp Leu Tyr Ala Asn Asn y Arg Arg Pro Thr Ala Ser Ile Asn Phe 100 105 110 gtg act gct cac gac ggc ttc acc ctc aat gac ttg gtc agt tac aac 384 Val Thr Al a His Asp 0 y Phe Thr Leu Asn Asp Leu Val Ser Tyr Asn 115 120 125 gag aag cac aac atg gcc aac ggt gaa gac ggt cgg gac ggt gaa tca 432 O u Lys Hi s Asn Wbt Al a Asn 0 y 0 u Asp 0 y Arg Asp 0 y 0 u Ser 130 135 140 cac aac cgt t cc tgg aac tgt ggc gtc gaa gga cca act gac gat cct 480 His Asn Arg Ser Trp Asn Cys O y Val O u O y Pro Thr Asp Asp Pro 145 150 155 160 gag att atg cag ctg cgt gct cag caa cga cgc aac ttc ctc acc acc 528 Su Ile 'b t n Leu Arg Ala n O n Arg Arg Asn Phe Leu Thr Thr 165 170 175 ttg ttg ctg tcc cag ggc acc cct atg ttg tcc cac ggt gat gaa atg 576 Leu Leu Leu Ser O n Oy Thr Pro 'b t Leu Ser His O y Asp O u 'b t 180 185 190 gcc cgt acc caa aac ggc aac aac aac gtc tac tgc caa gac aat gaa 624 Ala Arg Thr 3n Asn 3y Asn Asn Asn Val Tyr Cys 3n Asp Asn 3u 195 200 205 ctg gcg tgg gtg aat tgg gat cag gct gaa gaa aac gct gac ttg gtg 672 Leu Ala Trp Val Asn Trp Asp n Ala u u Asn Ala Asp Leu Val 210 215 220 agc ttc acc agg cgt ttg ctg cgt atc cga gca aac cac cca gta ttt 720 Ser Phe Thr Arg Arg Leu Leu Arg Ile Arg Ala Asn His Pro Val Phe 225 230 235 240 agg cgc agg cag ttc ctt gcc ggt ggc cct ttg ggc gcc gat gtt cgt 768 Arg Arg Arg n Phe Leu Ala y y Pro Leu y Ala Asp Val Arg 245 250 255 Page 467 Gene Sequence 24-2-06.txt gac Asp gat Asp ggc Gy gac Asp 305 aat Asn acc Thr ggc Gy gt g Val aag Lys 385 gaa G u ct c Leu cat Hi s caa G n gac Asp 465 gat atc gca tgg ctg gta cca aat gga Asp lie Ala Trp Leu Val Pro Asn Gy acc ttg atg act caa Thr Leu let Thr G n 270 tgg Tr p 275 gcc Al a ttc Phe cct Pr o gaa G u acc Thr 355 gct Al a gag G u gaa G u ctt Leu cac Hi s 435 ct g Leu gag G u ttc Phe gaa G u ttg Leu gag G u 325 gt g Val act Thr gac Asp gaa G u gcg Al a 405 gca Al a gct Al a caa G n gac Asp gct Al a gag G u at g bet 310 cat Hi s ggc Gy gtt Val tac Ty r ctt Leu 390 gca Al a gaa G u gat Asp gat Asp act Thr 470 ttc Phe cct Pr o 295 ttc Phe ttc Phe cac Hi s cct Pr o acc Thr 375 gcg Al a gca Al a cgt Ar g gcg Al a gaa G u 455 gaa G u ggt Gy 280 gat Asp aac Asn ggt Gy ccg Pr o gcc Al a 360 aag Lys gca Al a aag Lys gct Al a at t l Ie 440 gt a Val tct Ser 265 aaa Lys t at Tyr gct Al a at g Net ct g Leu 345 cgt Ar g ctt Leu gag G u gaa G u tcg Ser 425 gcc Al a gcg Al a gac Asp t ca Ser cga Ar g cac Hi s aag Lys 330 gag G u t cc Ser gag G u aag Lys gct Al a 410 act Thr gat Asp gca Al a t cc Ser ct g Leu gga Gy ttc Phe 315 tgg Tr p gat Asp acg Thr gaa G u gaa G u 395 gaa G u cag G n gag G u gag G u gag G u 475 cag G n cag G n 300 gaa G u aag Lys ct c Leu at g bet aag Lys 380 gct Al a gat Asp gaa G u gt a Val gt c Val 460 cag G n gt g Val 285 aaa Lys cct Pr o ctt Leu acc Thr ct g Leu 365 at c SIle gct Al a gct Al a gct Al a gcg Al a 445 gag G u gct Al a ttc Phe at c SIle at c SIle ttg Leu at c SIle 350 ct g Leu gct Al a gag G u gct Al a gaa G u 430 gaa G u act Thr gag G u ttc Phe cac Hi s gat Asp gt c Val 335 gaa G u cgc Ar g gct Al a aag Lys gag G u 415 ttg Leu gaa G u gag G u gt a Val aac Asn gat Asp ttc Phe 320 gat Asp gct Al a cag G n gaa G u cgc Ar g 400 gct Al a gcc Al a cca Pr o ccc Pr o gct Al a 480 864 912 960 1008 1056 1104 1152 1200 1248 1296 1344 1392 1440 tca gag gag cct gaa gcg gac gaa gaa Ser G u G u Pro G u Ala Asp G u G u 485 cgc <210> 366 <211> 491 <212> PRT <213> Cor ynebact eri um gl ut am cum gag aag G u Lys 490 tagtacaccg aaagtggcgt 1493 1496 Page 468 ene Sequence 24- 2- 06. txt <400> 366 vb t O31u O31n ValI O31u O31u As p ValI G u Hi s 145 O31u Le u A a Le u Ser 225 Ar g As p As p O31y As p 305 As n Th r Hi s Val As p Phe Asp As p Asp Pr o Val O31y (1u (1y Tr p As n O31y Pr o Ala Thr Leu Ty r A a 1 00 Thr A a Hi s 115 Lys Hi s As n 130 As n Ar g Ser I Ie 'b t O31n Leu Leu Ser 1 80 Ar g Thr O31n 1 95 Ala Tr p Val 210 Phe Thr Ar g Ar g Ar g O31n Ar g Asp Ilie 260 Asp Trp Asp 275 Asp A a Il e 290 Ser Phe Ilie Leu Pr o Pr o Thr 03 u A a 340 O31y ValI ValI O31y Lys5 Le u As n As p vb t Tr p Le u 1 65 O31n As n As n Ar g Ph e 245 A a Ph e O31u Le u O31u 325 ValI Phe Arg Phe Asp Leu Ala Ser Thr Leu A a As p Ser Tyr Tyr 70 O31y As n O31y A a As n 150 Ar g O31y O31y Tr p Le u 230 Le u Tr p A a O31u vb t 310 Hi s (31y Ar g O31n O31n 55 Ar g O31u O31y Ph e As n 135 cys A a Thr As n As p 215 Le u A a Le u Ph e Pr o 295 Ph e Ph e Hi s A a Lys5 O31y Thr A a Ar g 1 Le u G u ValI O31n vb t 1 As n A a I Ie O31y Pr o 265 Lys5 Tyr A a vb t Le u 345 Ph e I Ie Ph e Ar g Ar g Thr As p G y 0Oy 1 Ar g Ser Tyr 0Ou A a 235 Le u 0Oy Le u 0Oy Ph e 315 Tr p As p Ph e A a Pr o As p Le u A a Le u Ar g 140 Pr o As n Hi s cys As n 220 As n O31y Thr O31n O31n 300 O31u Lys5 Le u As p O31u Pr o Ph e Thr Ser ValI 125 As p Thr Ph e O31y O31n 205 A a Hi s A a Le u ValI 285 Lys5 Pr o Le u Thr ValI Tr p Tr p Ar g Ser As n Tyr G u As p Thr 1 0Ou As n Le u ValI ValI 255 Thr Ph e Hi s As p ValI 335 0Ou Oy Oy Thr lieThr Val Pro Ala Arg Ser Thr I'bt Leu Leu Ar g O31n Page 469 Val Lys 385 G u Leu Hi s G n Asp 465 Ser 355 Al a G u G u Leu Hi s 435 Leu G u G u Asp G u Al a 405 Al a Al a G n Asp G u 485 Tyr Leu 390 Al a G u Asp Asp Thr 470 Al a Thr 375 Al a Al a Ar g Al a G u 455 G u Asp Gene Sequence 24-2-06. t xt 360 365 Lys Leu G u G u Lys lie Ala 380 Ala G u Lys G u Ala Ala G u 395 Lys G u Ala G u Asp Ala Ala 410 Ala Ser Thr n G u Ala G u 425 430 lie Ala Asp u Val Ala u 440 445 Val Ala Ala G u Val G u Thr 460 Ser Asp Ser G u G n Ala G u 475 G u G u G u Lys 490 Al a Lys G u 415 Leu G u G u Val G u Ar g 400 Al a Al a Pr o Pr o Al a 480 <210> 367 <211> 547 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(547) <223> RXA01111 <400> 367 agaaaaccct gccgatgcaa actttgagga gagattcaat ggtgggtcta gacgcgcttg ggcatataag atgacgttcc gag G u acc Thr gag G u at t SIle ggc Gy aac Asn tcg Ser gac Asp gtt Val ct c Leu caa G n gat Asp caa G n gct Al a ct g Leu gag G u gga Gy ggt Gy gtt Val gga Gy tgt Cys cgc Ar g cag G n 75 aag Lys tgg Tr p acg Thr ct a Leu gat Asp cgc Ar g cgg Ar g cct Pr o aac Asn tta Leu 45 gcc Al a t ac Tyr tgc Cys gga Gy ttc Phe gat Asp cac Hi s gga Gy gac Asp cat gct Hi s Al a 15 gca ct c Al a Leu gca gat Al a Asp att tgg lie Trp ttc cga Phe Ar g 80 gcg aac Al a Asn 95 Page 47C caaggaatag aaatcattct atg aca tca acg att IVet Thr Ser Thr Ile 1 tat cct ctg ggt tca Tyr Pro Leu y Ser ttc tcc gac gtt gca Phe Ser Asp Val Ala aac aac gag act cga Asn Asn G u Thr Arg cat tgc tac ctt cct His Cys Tyr Leu Pro gtt cat ggc ccg tgg Val His G y Pro Trp aaa ctt cta gtt gat Lys Leu Leu Val Asp 100 Gene Sequence 24- 2- 06. txt ccc tat gct cgt gct ttc gat gga gat ttt gat gga cat ccg tca cta 451 Pro Tyr Ala Arg Ala Phe Asp G y Asp Phe Asp G y His Pro Ser Leu 105 110 115 ttt tct tac gac atc acc aat cca aat gac ccc aac ggt cgc aat acc 499 Phe Ser Tyr Asp I e Thr Asn Pro Asn Asp Pro Asn y Arg Asn Thr 120 125 130 gaa gac agc att gat cac aca atg aag tct gtc gtg gtg aac cca ttc 547 G u Asp Ser Ile Asp His Thr IVet Lys Ser Val Val Val Asn Pro Phe 135 140 145 <210> 368 <211> 149 <212> PRT <213> Corynebacter i um gl utam cum <400> 368 et Thr Ser Thr IIe G u His Ser Tyr G n Val Trp Pro G y His Ala 1 5 10 Tyr Pro Leu y Ser Thr Tyr Asp y Ala y Thr Asn Phe Ala Leu 25 Phe Ser Asp Val Ala G u Arg Val G u Leu Cys Leu Leu Asp Ala Asp 40 Asn Asn G u Thr Arg lie Pro Leu G u G u Arg Asp Ala His Ile Trp 55 His Cys Tyr Leu Pro G y Val G n Pro G y G n Arg Tyr G y Phe Arg 70 75 Val His G y Pro Trp Asn Pro Asp G u G y Lys Arg Cys Asp Ala Asn 90 Lys Leu Leu Val Asp Pro Tyr Ala Arg Ala Phe Asp y Asp Phe Asp 100 105 110 G y His Pro Ser Leu Phe Ser Tyr Asp lie Thr Asn Pro Asn Asp Pro 115 120 125 Asn G y Arg Asn Thr G u Asp Ser IIe Asp His Thr IVet Lys Ser Val 130 135 140 Val Val Asn Pro Phe 145 <210> 369 <211> 1635 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1612) <223> RXN01550 <400> 369 ttcgccagca gtacttcttc acctctgctt ccctgcaggc catgattcag ggccacctgg cgcaccacaa ggacctcagc aactttgccg agttcactcc gtg cag ctc aat gac 115 Val G n Leu Asn Asp 1 act cac cca gtg ttg gct atc cct gag ctt atg cgt ctg ctc atg gac 163 Page 471 Thr His Pro Val Leu Ala lle Gene Sequence 24- 2- 06. txt Pro G u Leu IVet Arg Leu Leu IVet Asp 15 gag G u ttc Phe gat Asp aca Thr gat Asp cat Hi s gca Al a t ac Tyr cca Pr o 150 act Thr aag Lys cgc Ar g gag G u at t SIle 230 gt a Val cca Pr o gac Asp t ac Tyr cag G n at c SIle gag G u gca Al a ct g Leu 120 ct g Leu cgt Ar g ctt Leu ct g Leu at c SIle 200 cag G n cgc Ar g gac Asp cgc Ar g ggc G y aac Asn ttc Phe cgc Ar g at c SIle at t l Ie acc Thr cca Pr o ct g Leu ggt Sy 170 t cc Ser gct Al a at t SIle cac Hi s t ac Tyr 250 gt c Val tgg Tr p cac Hi s caa G n cgc Ar g 75 gac Asp gcc Al a gag G u gag G u cgc Ar g 155 t cc Ser t at Ty r gct Al a gag G u gag G u 235 tt c Phe at c SIle gaa G u acc Thr cag G n ttc Phe cgc Ar g tgt Cys at c SIle aag Lys 140 at g et gat Asp gcc Al a aat Asn at t SIle 220 t ac Tyr cgt Ar g ttt Phe gaa G u gt g Val 45 ct g Leu cgt Ar g at g Net t ac Tyr at c SIle 125 ttc Phe at c SI e gat Asp gac Asp aag Lys 205 gat Asp aag Lys at t SIle ggc G y t cc Ser ct c Leu ttc Phe ttg Leu gct Al a gcg Al a 110 aag Lys aac Asn aac Asn tgg Tr p gat Asp 190 caa G n cca Pr o cgc Ar g aag Lys gcc Al a 270 tgg Tr p acc Thr tgg Tr p gag G u cca Pr o 95 gca Al a gcc Al a aac Asn cca Pr o gt a Val 175 aag Lys gac Asp gaa G u cag G n gaa G u 255 aag Lys gca Al a gaa G u cgc Ar g cgc Ar g at c SIle t at Tyr gag G u aag Lys ggt G y 160 acc Thr t cc Ser ttc Phe t cc Ser ct c Leu 240 gat Asp gcc Al a at c l Ie gct Al a gt g Val gca Al a cag G n t cc Ser acc Thr act Thr 145 ct g Leu gat Asp gt g Val gcc Al a at c SIle 225 at g Net ggc G y gcg Al a gt g Val ctt Leu tgg Tr p gcc Al a cac Hi s at c SIle ttg Leu 130 aac Asn tct Ser ct g Leu ctt Leu gag G u 210 ttt Phe aac Asn ct c Leu ccg Pr o aag Lys cag G n at c SIe gga G y act Thr 100 ggc G y gac Asp gtt Val ctg Leu gag G u 180 gaa G u at c SIe gtg Val ct c Leu gac Asp 260 t at Tyr cgc gcc aag gcg att atc aag ctc atc aac tct att gct gac ttg gta Page 472 Gene Sequence 24-2-06.txt Arg Ala Lys Ala lie lie Lys Leu Ile Asn Ser lie Ala Asp Leu Val 280 285 290 aac aac gat cct gag gtc tcc ccg ctg ctc aag gtg gtc ttt gta gag 1027 Asn Asn Asp Pro G u Val Ser Pro Leu Leu Lys Val Val Phe Val G u 295 300 305 aac tac aac gtc tcc cct gct gag cac atc ttg cct gcg tct gat gtc 1075 Asn Tyr Asn Val Ser Pro Ala G u His lie Leu Pro Ala Ser Asp Val 310 315 320 325 tcc gaa cag att tcc acc gcc ggc aag gaa gcc agc ggt acc tcc aac 1123 Ser G u n Ile Ser Thr Al a G y Lys G u Al a Ser G y Thr Ser Asn 330 335 340 atg aag ttc atg atg aac ggc gcc ctc acc ctg ggc acc atg gac ggc 1171 IVet Lys Phe IVet IVet Asn G y Al a Leu Thr Leu G y Thr IVet Asp G y 345 350 355 gcc aac gta gag atc gtg gat tct gtg ggc gag gaa aac gcc tat atc 1219 Ala Asn Val u Ile Val Asp Ser Val y u u Asn Ala Tyr Ile 360 365 370 ttc ggt gct cgc gtg gaa gaa ttg cca gcc ctg cgc gaa agc tac gag 1267 Phe y Ala Arg Val G u G u Leu Pro Ala Leu Arg u Ser Tyr G u 375 380 385 cca tat gag ctc tat gag acc gtc cct ggc ctc aag cgc gca ttg gac 1315 Pro Tyr G u Leu Tyr G u Thr Val Pro y Leu Lys Arg Ala Leu Asp 390 395 400 405 gcc ctg gat aac ggc acc ctc aac gac aac aac agt ggt ttg ttc tac 1363 Ala Leu Asp Asn G y Thr Leu Asn Asp Asn Asn Ser G y Leu Phe Tyr 410 415 420 gac ctc aag cat tcc ttg atc cac ggt tat gga aaa gac gcc agc gac 1411 Asp Leu Lys His Ser Leu lie His y Tyr y Lys Asp Ala Ser Asp 425 430 435 acc tac tac gtg ctt ggc gat ttc gca gat tac cgc gag acc cgc gac 1459 Thr Tyr Tyr Val Leu G y Asp Phe Ala Asp Tyr Arg G u Thr Arg Asp 440 445 450 cgt atg gcc gcc gac tac gcc tcc gat ccc ctg ggt tgg gca cgc atg 1507 Arg IVet Ala Ala Asp Tyr Ala Ser Asp Pro Leu G y Trp Ala Arg IVet 455 460 465 gcc tgg atc aac att tgc gag tcc ggc cgt ttc tcc tcc gac cgc acc 1555 Ala Trp lie Asn Ile Cys u Ser y Arg Phe Ser Ser Asp Arg Thr 470 475 480 485 atc cgc gat tat gcc acc gag atc tgg aag ctc gag cca act cct gct 1603 lie Arg Asp Tyr Ala Thr G u lie Trp Lys Leu G u Pro Thr Pro Ala 490 495 500 gtt aag aag taggttttaa cctccgcttc taa 1635 Val Lys Lys <210> 370 <211> 504 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 370 Val G n Leu Asn Asp Thr His Pro Val Leu Ala lie Pro G u Leu Iet 1 5 10 Page 473 ene Sequence 24- 2- 06. txt Ar g I Ie A a ValI A a O31n Ser Th r Th r 145 Le u As p ValI A a I Ie 225 vb t G31y A a I Ie ValI 305 Pr o Ser G31y Leu Leu l'bt Asp 03 u Hi s Asp l'bt G31y Tr p O31u O31u Se r Tr p Ala Ph e O31u O31u As p G31y As n 115 A a (31y As p As p O31u 1 95 Tr p As p A a Th r G31y 275 As p Ph e Ser Th r vb t 355 Th r Tr p I Ie Le u ValI ValI Tr p Th r Le u 1 65 Le u Le u Le u O31n Ty r 245 li1e ValI ValI O31u ValI 325 As n O31y Ph e As p Th r 70 As p Hi s A a Ty r Pr o 150 Th r Ly s Ar g Ou le 230 ValI Pr o Ar g As n As n 310 Ser vb t A a A a O31n O31u O31u vb t A a A a 135 Ar g Ar g Ly s A a Ar g 215 Ly s Le u A a A a As n 295 Ty r O31u Ly s As n Tyr Thr 40 O31n le I I e As p O3 u Thr Ala Tr p 1 05 Leu Hi s 120 Leu Trp Ar g Tr p Leu Ser Leu Arg 1 85 1I e Lys 200 O31n (1y Arg Leu Asp Leu Ar g Thr 265 Lys A a 280 As p Pr o As n Val O31n I Ie Phe l'b t 345 Val O31u 360 Hi s O31n Ar g 75 As p A a O31u O31u Ar g 1 55 Ser Tyr A a O31u O31u 235 Ph e I Ie I Ie ValI Pr a 315 Thr As n ValI Thr O31n Ph e Ar g cys Ly s 140 vb t As p A a As n I Ie 220 Tyr Ar g Ph e Ly s Ser 300 A a A a O31y As p ValI Le u Ar g vb t Tyr I Ie 125 Ph e I Ie As p As p Ly s 205 As p Ly s I Ie O31y Le u 285 Pr a O31u O31y A a Ser 365 Le u Ph e Le u A a A a 110 Ly s As n As n Tr p As p 1 O31n Pr a Ar g Ly s A a 270 li1e Le u Hi s Ly s Le u 350 ValI Thr Tr p O31u Pr a A a A a As n Pr a ValI 1 Ly s As p O31u O31n O31u 255 Ly s As n Le u I Ie O31u 335 Thr O31y O31u Ar g Ar g li1e Tyr O31u Ly s G31y 1 Thr Ser Ph e Ser Le u 240 As p A a Ser Ly s Le u 320 A a Le u O31u O31u As n Ala Ty r I Ie Ph e O31y Ala Ar g Va 1O31u O31u L eu Pr o Ala Le u Page 474 Gene Sequence 24-2-06.txt 370 375 380 Arg u Ser Tyr G u Pro Tyr G u Leu Tyr u Thr Val Pro Gy Leu 385 390 395 400 Lys Arg Ala Leu Asp Ala Leu Asp Asn y Thr Leu Asn Asp Asn Asn 405 410 415 Ser G y Leu Phe Tyr Asp Leu Lys His Ser Leu lie His G y Tyr G y 420 425 430 Lys Asp Ala Ser Asp Thr Tyr Tyr Val Leu G y Asp Phe Al a Asp Tyr 435 440 445 Arg G u Thr Arg Asp Arg IVet Ala Ala Asp Tyr Ala Ser Asp Pro Leu 450 455 460 Sy Trp Ala Arg IVet Ala Trp Ile Asn Ile Cys Gu Ser y Arg Phe 465 470 475 480 Ser Ser Asp Arg Thr Ile Arg Asp Tyr Ala Thr G u Ile Trp Lys Leu 485 490 495 G u Pro Thr Pro Ala Val Lys Lys 500 <210> 371 <211> 1367 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (1)..(1344) <223> FRXA01550 <400> 371 atc ttc caa cag ctg ttc tgg cgc gtg tgg gaa atc atc aca gag atc 48 Ile Phe n n Leu Phe Trp Arg Val Trp Gu Ile Ile Thr Gu Ile 1 5 10 gat cgc cgc ttc cgt ttg gag cgc gca gcc gat gga ctg gat gaa gag 96 Asp Arg Arg Phe Arg Leu G u Arg Ala Ala Asp y Leu Asp G u G u 25 acc atc gac cgc atg gct cca atc cag cgc ggc act gtt cat atg gca 144 Thr Ile Asp Arg IVet Ala Pro Ile Gn Arg Gy Thr Val His IVet Ala 40 tgg att gcc tgt tac gcg gca tat tcc atc aat ggc gtg gca gcg ctg 192 Trp lie Ala Cys Tyr Al a Ala Tyr Ser Ile Asn y Val Ala Ala Leu 55 cac acc gag atc atc aag gcc gag acc ttg gct gac tgg tac gca ctg 240 His Thr Gu lie lie Lys Ala u Thr Leu Ala Asp Trp Tyr Ala Leu 70 75 tgg cca gag aag ttc aac aac aag act aac ggt gtt acc cca cgc cgt 288 Trp Pro G u Lys Phe Asn Asn Lys Thr Asn y Val Thr Pro Arg Arg 90 tgg ctg cgc atg atc aac cca ggt ctg tct gac ctg ctc act cga ctt 336 Trp Leu Arg IVet lie Asn Pro y Leu Ser Asp Leu Leu Thr Arg Leu 100 105 110 tcc ggt tcc gat gat tgg gta acc gat ctg gat gag ctg aag aag ctg 384 Ser y Ser Asp Asp Trp Val Thr Asp Leu Asp G u Leu Lys Lys Leu Page 475 Gene Sequence 24-2-06.txt cgc Ar g aag Lys 145 ggc Gy ct c Leu ctt Leu act Thr gcg Al a 225 cct Pr o gt c Val at t SIle at g Net gag G u 305 cgc Ar g ct c Leu aac Asn cat Hi s t at Tyr gct Al a gag G u gag G u ttc Phe 195 at c SIle at c l Ie gt c Val cct Pr o acc Thr 275 aac Asn gt g Val gaa G u gag G u acc Thr 355 ttg Leu gac Asp aag Lys gat Asp 165 aag Lys at t SIle ggc Gy ct c Leu ccg Pr o 245 gag G u ggc Gy gcc Al a tct Ser ttg Leu 325 gt c Val aac Asn cac Hi s gat Asp caa G n 150 cca Pr o cgc Ar g aag Lys gcc Al a at c SIle 230 ct g Leu cac Hi s aag Lys ct c Leu gt g Val 310 cca Pr o cct Pr o gac Asp ggt Gy aag Lys 135 gac Asp gaa G u cag G n gaa G u aag Lys 215 aac Asn ct c Leu at c SIle gaa G u acc Thr 295 ggc Gy gcc Al a ggc Gy aac Asn t at Tyr 375 120 t cc Ser ttc Phe t cc Ser ct c Leu gat Asp 200 gcc Al a tct Ser aag Lys ttg Leu gcc Al a 280 ct g Leu gag G u ct g Leu ct c Leu aac Asn 360 gga Gy gtg ctt Val Leu gcc gag Al a G u atc ttt Ile Phe 170 atg aac IVet Asn 185 ggc ctc G y Leu gcg ccg Ala Pro att gct I e Al a gtg gt c Val Val 250 cct gcg Pro Al a 265 agc ggt Ser G y ggc acc G y Thr gaa aac Su Asn cgc gaa Arg G u 330 aag cgc Lys Ar g 345 agt ggt Ser G y aaa gac Lys Asp gaa G u tgg Tr p 155 gac Asp gcg Al a acc Thr ggt Gy gac Asp 235 ttt Phe tct Ser acc Thr at g Net gcc Al a 315 agc Ser gca Al a ttg Leu gcc Al a gaa G u 140 at c SIle gt g Val ct c Leu gac Asp t at Tyr 220 ttg Leu gt a Val gat Asp t cc Ser gac Asp 300 t at Tyr t ac Tyr ttg Leu ttc Phe agc Ser 380 125 ct c Leu ct c Leu cag G n t ac Tyr at c SIle 205 gt c Val gt a Val gag G u gt c Val aac Asn 285 ggc Gy at c SIle gag G u gac Asp t ac Tyr 365 gac Asp cgc Ar g gag G u at t SIle gt a Val 190 cca Pr o cgc Ar g aac Asn aac Asn t cc Ser 270 at g Net gcc Al a ttc Phe cca Pr o gcc Al a 350 gac Asp acc Thr gct Al a cgc Ar g aag Lys 175 ct a Leu gca Al a gcc Al a aac Asn t ac Tyr 255 gaa G u aag Lys aac Asn ggt Gy t at Tyr 335 ct g Leu ct c Leu t ac Tyr at c SIe cag G n 160 cgc Ar g gac Asp cgc Ar g aag Lys gat Asp 240 aac Asn cag G n ttc Phe gt a Val gct Al a 320 gag G u gat Asp aag Lys t ac Tyr 432 480 528 576 624 672 720 768 816 864 912 960 1008 1056 1104 1152 1200 gtg ctt ggc gat ttc gca gat tac cgc gag Val Leu y Asp Phe Ala Asp Tyr Arg u acc cgc gac cgt atg gcc Thr Arg Asp Arg IVet Ala Page 476 385 390 gcc gac tac gcc tcc gat ccc ctg g Ala Asp Tyr Ala Ser Asp Pro Leu C 405 aac att tgc gag tcc ggc cgt ttc t Asn Ile Cys u Ser y Arg Phe S 420 4 tat gcc acc gag atc tgg aag ctc g Tyr Ala Thr G u lie Trp Lys Leu C 435 440 taggttttaa cctccgct t c taa <210> 372 <211> 448 <212> PRT <213> Corynebacter i um gl ut ai cum Sequence 24-2-06.txt 395 400 Igt tgg gca cgc atg gcc tgg atc 3y Trp Ala Arg IVet Ala Trp Ile 410 415 cc tcc gac cgc acc atc cgc gat ier Ser Asp Arg Thr lie Arg Asp .25 430 lag cca act cct gct gtt aag aag Su Pro Thr Pro Ala Val Lys Lys 445 1248 1296 1344 1367 <400> 372 Ile Phe n n Leu Phe Trp 1 Asp Thr Tr p Hi s Tr p Tr p Ser Ar g Lys 145 Sy Leu Leu Thr Al a 225 Ar g Asp Al a G u G u Ar g Ser 115 Tyr Al a G u G u Phe 195 I e l e 5 Ar g et Tyr SIle Phe I le Asp Asp Lys Asp 165 Lys SIle Sy Leu Leu Al a Al a Lys 70 Asn Asn Tr p Asp G n 150 Pr o Ar g Lys Al a SIle 230 G u Pr o Al a Al a Asn Pr o Val Lys 135 Asp G u G n G u Lys 215 Asn Ar g Ar g SIle 40 Tyr G u Lys Sy Thr 120 Ser Phe Ser Leu Asp 200 Al a Ser Val Al a G n Ser Thr Thr Leu 105 Asp Val Al a SIle Met 185 Sy Al a SIle Tr p 10 Al a Ar g SIle Leu Asn 90 Ser Leu Leu G u Phe 170 Asn Leu Pr o G u Asp Sy Asn Al a Sy Asp Asp G u Tr p 155 Asp Al a Thr Sy SIle Leu Val Val Tr p Thr Leu Leu 125 Leu Leu G n Tyr SIle 205 Val G u G u et Al a Al a Ar g Ar g Lys Al a Ar g Lys 175 Leu Al a Al a Al a Asp 235 Leu Val Asn Asn Page 477 Pro u Val Ser Pro Leu Leu 245 Gene Sequence 24-2-06.txt Lys Val Val Phe Val G u Asn Tyr Asn 255 Val SIle Met G u 305 Ar g Leu Asn Hi s Val 385 Al a Asn Tyr Pr o Thr 275 Asn Val G u G u Thr 355 Leu Gy Tyr Cys Thr 435 G u Gy Al a Ser Leu 325 Val Asn Hi s Phe Ser 405 Ser SIle SIle G u Thr 295 Gy Al a Gy Asn Tyr 375 Asp Pr o Ar g Lys Leu Al a 280 Leu G u Leu Leu Asn 360 Gy Tyr Leu Phe Leu 440 Pr o 265 Ser Gy G u Ar g Lys 345 Ser Lys Ar g Gy Ser 425 G u 250 Al a Gy Thr Asn G u 330 Ar g Gy Asp G u Tr p 410 Ser Pr o Ser Thr bet Al a 315 Ser Al a Leu Al a Thr 395 Al a Asp Thr Val Asn 285 Gy l Ie G u Asp Tyr 365 Asp Asp et Thr Al a 445 Ser 270 et Al a Phe Pr o Al a 350 Asp Thr Ar g Al a SIle 430 Val G u Lys Asn Gy Tyr 335 Leu Leu Tyr bet Tr p 415 Ar g Lys <210> 373 <211> 2348 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (1)..(2325) <223> RXN02100 <400> 373 cta ggt cga atc aac gcc gag gag caa Leu Gy Arg lie Asn Ala Gu Gu n 1 5 gac aag ctg tgg tac cag gac acc gca Asp Lys Leu Trp Tyr G n Asp Thr Ala 25 gat cca ctc gtt gcg tac ttc tcc atg Asp Pro Leu Val Ala Tyr Phe Ser IVet 40 ctg cca atc tac tct ggc gga ctt ggt Leu Pro lie Tyr Ser Gy y Leu y aac ctc agc gaa tac ctc agc Asn Leu Ser G u Tyr Leu Ser 10 gat gca acc gat gct gtc gga Asp Ala Thr Asp Ala Val y gag ttt ggc att cac cca agc G u Phe G y IIe His Pro Ser gtg ctt gcg ggc gag aac atg Val Leu Ala Gy u Asn IVet Page 478 Gene Sequence 24-2-06.txt aag Lys t ac Tyr cag G n gtt Val ggt Gy at c SIle 145 ct c Leu at c SIle gca Al a aac Asn at c SIle 225 t cc Ser ttc Phe gat Asp ggt Gy cgc Ar g 305 agc Ser gca Al a cac Hi s gag G u gat Asp 115 cag G n ttg Leu aac Asn cag G n tgc Cys 195 ggc Sy gag G u at c SIle at g bet cag G n 275 gag G u agc Ser gac Asp gac Asp t ac Tyr aag Lys aac Asn gt a Val ct g Leu act Thr 165 ct c Leu gct Al a gca Al a at g let acc Thr 245 at g let tgc Cys gat Asp cat Hi s ttc Phe tt g Leu 70 tt c Phe tac Ty r ggc Sy aag Lys ctt Leu 150 gac Asp gtt Val cgt Ar g ggt G y gag G u 230 acc Thr gt g Val gtt Val cca Pr o gct Al a 310 gcc Al a ggt G y acc Thr cac Hi s gag G u at t SIle 135 gat Asp cgc Ar g ct c Leu ggt G y ttc Phe 215 t ac Tyr cac Hi s cgt Ar g ggt Sy cac Hi s 295 aat Asn ggc Gy gt g Val cag G n gat Asp cag G n 120 gca Al a acc Thr ct g Leu ggt G y ct g Leu 200 ct g Leu cca Pr o acc Thr cgt Ar g gtt Val 280 cgc Ar g ggc Sy ct g Leu cca Pr o t ca Ser cca Pr o 105 gt c Val ct g Leu aac Asn t ac Tyr gtt Val 185 aag Lys acc Thr gca Al a cca Pr o t at Tyr 265 cca Pr o ttc Phe gt c Val t ac Tyr ct g Leu ct g Leu 90 gca Al a act Thr tgg Tr p at c SIle ggt G y 170 ggt G y cgc Ar g ct g Leu gca Al a gt c Val 250 ct c Leu at t SIle aac Asn gca Al a at c I e 75 t cc Ser gaa G u gtt Val gt a Val gag G u 155 ggc G y ggc G y t ca Ser gag G u ttc Phe 235 cca Pr o ggt Sy gag G u at g Met aag Lys 315 ggt G y ggt Sy ct g Leu tct Ser gca Al a 140 gca Al a gac Asp gt c Val tct Ser cgt Ar g 220 gag G u gca Al a ggc Sy aag Lys gct Al a 300 ctt Leu gtt Val gac Asp ccg Pr o gt c Val 125 aac Asn aac Asn aat Asn cgc Ar g gtt Val 205 at c SIle cag G n ggc Sy ggt Sy gca Al a 285 cat Hi s cat Hi s ggt G y ggt Sy at t SIle 110 acc Thr gtt Val cca Pr o gag G u gct Al a 190 gca Al a cgc Ar g gtt Val at c SIle cag G n 270 ctt Leu at g bet ggt Sy ttg Leu tgg Tr p gag G u t ac Tyr ggc Sy gaa G u cac Hi s 175 gt c Val cac Hi s gag G u cgt Ar g gac Asp 255 cca Pr o gag G u ggc Sy gaa G u ct c Leu cag G n gca Al a cca Pr o cgc Ar g gag G u 160 cgc Ar g aac Asn ct c Leu cgc Ar g gcg Al a 240 cgc Ar g gaa G u ctt Leu ctt Leu gt a Val 320 240 288 336 384 432 480 528 576 624 672 720 768 816 864 912 960 1008 ccc gga Pro G y tat gag cct cgt gaa Tyr u Pro Arg u Page 479 Gene Sequence 24-2-06.txt gt g Val aag Lys ctt Leu aag Lys 385 gct Al a gca Al a at t SIle ct g Leu cca Pr o 465 ggc Gy gt c Val agc Ser cct Pr o ggc Gy 545 gag G u tgc Cys gaa G u at c SIle gag G u 355 gtt Val tgg Tr p gct Al a ctt Leu ttc Phe 435 aac Asn cag G n aag Lys gac Asp ct g Leu 515 gaa G u acc Thr acc Thr gac Asp gca Al a 325 cac Hi s aag Lys gat Asp gt g Val act Thr 405 tgg Tr p cgt Ar g gaa G u gtt Val at g Met 485 ttc Phe tct Ser tcg Ser t cc Ser tgg Tr p 565 ct g Leu ct g Leu gt c Val gaa G u tct Ser cgc Ar g 390 gca Al a acc Thr cgc Ar g cgc Ar g at t SIle 470 cag G n ct c Leu ggt Gy gga Gy at c SIle 550 acc Thr gaa G u ttt Phe acc Thr ct c Leu tgg Tr p 375 aac Asn aag Lys t cc Ser gt a Val ct g Leu 455 gct Al a gaa G u ttc Phe gct Al a acc Thr 535 tct Ser at c SIle t cc Ser t ac Tyr aac Asn at c SIle 360 t ca Ser aag Lys t cc Ser cgc Ar g t cc Ser 440 cgt Ar g ggt Gy at c SIle ct g Leu gac Asp 520 t cc Ser gat Asp cca Pr o cag G n aag Lys 330 ggt gtt cac G y Val Hi s 345 gat cgc gt c Asp Arg Val aac cca cag Asn Pro n ttc cgt gct Phe Ar g Al a 395 tgg tct cac Trp Ser His 410 gtt ctg gat Val Leu Asp 425 acc tac aag Thr Tyr Lys tcc atc ttg Ser Ile Leu aag gca cac Lys Al a Hi s 475 gtc cac ttc Val Hi s Phe 490 cct gat t ac Pro Asp Tyr 505 gtg tgg ctg Val Trp Leu ggt atg aag G y Iet Lys ggt tgg tgg G y Trp Trp 555 acc gtt gag Thr Val G u 570 gcg ctg t ac Ala Leu Tyr 585 cgc gac aag Arg Asp Lys ct g Leu act Thr gct Al a 380 gac Asp cgt Ar g cca Pr o cgc Ar g ctt Leu 460 cca Pr o gct Al a gac Asp aac Asn gcc Al a 540 gat Asp t cc Ser gac Asp ccg Pr o ggc Gy 365 gt c Val ct a Leu gga Gy aac Asn ttg Leu 445 aat Asn cat Hi s gat Asp at c SIle aac Asn 525 gt c Val gaa G u cag G n ct g Leu 335 tgg Tr p gct Al a tct Ser gag G u acc Thr 415 ct g Leu ttg Leu gaa G u at g Net gct Al a 495 ct g Leu gt g Val aat Asn cct Pr o ttg Leu 575 gaa G u gt c Val gat Asp gag G u gtt Val 400 gaa G u acc Thr at g bet cgc Ar g ggt Gy 480 ggt Gy gcc Al a cgc Ar g ggt Gy aag Lys 560 gaa G u aac Asn 1056 1104 1152 1200 1248 1296 1344 1392 1440 1488 1536 1584 1632 1680 1728 1776 1824 aac ggc atc cca cag Asn Gy lie Pro Gn Page 480 Gene Sequence 24-2-06.txt 595 gac tgg ctg gac ctg gtt cgc gaa tcc Asp Trp Leu Asp Leu Val Arg u Ser 610 615 gtc acc tcc acc cgc atg gtg cgc gac Val Thr Ser Thr Arg Mbt Val Arg Asp 625 630 cca acc aaa cac cag gca gag ctc att Pro Thr Lys His G n Al a u Leu Ile 645 gat tac gcg gca tgg ctt gag cac atc Asp Tyr Ala Ala Trp Leu u His lie 660 665 aag gtc tca gac ctg aag atc agc gag Lys Val Ser Asp Leu Lys Ile Ser G u 675 680 ctt gaa gtc agc gtt cgc gtt gat tcc Leu G u Val Ser Val Arg Val Asp Ser 690 695 ttc caa gct cag gca ctc ttt ggt gcg Phe G n Al a n Al a Leu Phe G y Al a 705 710 gaa gat cca gaa atc acc gtt ttg acc G u Asp Pro G u lie Thr Val Leu Thr 725 gcg gca aag gtc agc act gac ctg cca Ala Ala Lys Val Ser Thr Asp Leu Pro 740 745 cgc gtt gtt cca aac aac agg atg ctg Arg Val Val Pro Asn Asn Arg IVet Leu 755 760 ctg atc acc tac ttg gag aac tagggcg Leu Ile Thr Tyr Leu G u Asn 770 775 <210> 374 <211> 775 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 374 Leu Gy Arg lie Asn Ala u u On 1 5 Asp Lys Leu Trp Tyr G n Asp Thr Ala 25 Asp Pro Leu Val Ala Tyr Phe Ser IVet 40 Leu Pro lie Tyr Ser Gy y Leu y 55 Lys Ser Ala Ser Asp Leu G y Val Pro 70 tgg Tr p t ac Tyr gcg Al a 650 aaa Lys agc Ser ggt Gy ct c Leu cca Pr o 730 ggc Gy gt c Val acc Thr acc Thr 635 cag G n gca Al a gcc Al a tcg Ser gga G y 715 cgc Ar g aac Asn agc Ser 605 acc ct g Thr Leu 620 acc cag Thr G n cct gca Pr o Al a gag tgg Su Trp atc acg lie Thr 685 ctt aac Leu Asn 700 cac aac His Asn ggc gat Sy Asp tac ggc Tyr G y cca gcg Pro Al a t ca Ser t ac Tyr gaa G u gct Al a 670 gcg Al a gac Asp ggt G y ggc G y at c SIle 750 gaa G u cca Pr o t ac Tyr gca Al a 655 ggc Sy cag G n gac Asp gac Asp gcc Al a 735 act Thr acc Thr at g Net cgc Ar g 640 gcg Al a gt c Val gag G u gag G u at c SIe 720 t ac Tyr gcc Al a cgc Ar g 1872 1920 1968 2016 2064 2112 2160 2208 2256 2304 2348 aaa ct agct t t ac caa Asn Leu Ser G u Tyr Leu Ser 10 Asp Ala Thr Asp Ala Val y G u Phe G y IIe His Pro Ser Val Leu Ala y u Asn IVet Leu Ile y Val y Leu Leu 75 Page 481 Tyr Thr His G1y Tyr Phe Thr ene Sequence 24- 2- 06. txt O31n Ser Leu Ser G31y Asp G31y 90 Trp O31n O31n ValI G31y I Ie 145 Le u I Ie A a As n I Ie 225 Ser Ph e As p G31y Ar g 305 Ser ValI Ly s Le u Ly s 385 A a A a I Ie O31u As p 115 O31n Le u As n O31n cys 1 95 O31y Ou le vb t O31n 275 O31u Ser As p I Ie O31u 355 ValI Tr p A a Le u Ph e 435 Ly s As n ValI Le u Thr 1 65 Le u A a A a vb t Thr 245 vb t cys As p Hi s Ph e 325 Hi s Ly s As p ValI Thr 405 Tr p Ar g Tyr O31y Ly s Le u 150 As p ValI Ar g O31y O31u 230 Th r ValI ValI Pr o A a 310 A a ValI O31u Ser Ar g 390 A a Thr Ar g Hi s O31u I Ie 135 As p Ar g Le u O31y Ph e 215 Tyr Hi s Ar g O31y Hi s 295 As n O31y Thr Le u Tr p 375 As n Ly s Ser ValI Pro Ala 1 05 Val Thr Leu Trp As n I I e Ty r 03 y 1 70 Val O31y 1 85 Lys Ar g Thr Leu A a A a Pr o Val 250 Tyr Leu 265 Pr o I Ie Phe As n Val Ala Ty r Pr o 330 O3 y Val 345 Asp Arg As n Pr o Phe Ar g Tr p Ser 410 Val Leu 425 Thr Tyr O31u ValI ValI O31u 1 O31y O31y Ser O31u Ph e 235 Pr o O31y O31u Ly s 315 O31y Hi s ValI O31n A a 395 Hi s As p Le u Ser A a 140 A a As p ValI Ser Ar g 220 O31u A a O31y Ly s A a 300 Le u Tyr Le u Thr A a 380 As p Ar g Pr o Pr o ValI 125 As n As n As n Ar g ValI 205 li1e O31n O31y O31y A a 285 Hi s Hi s O31u Pr o O31y 365 ValI Le u O31y As n I Ie 110 Thr ValI Pr o O31u A a 1 A a Ar g ValI I Ie O31n 270 Le u O31y Pr o Thr 350 O31y O31u ValI Hi s ValI 430 O31u Tyr O31y O31u Hi s 1 ValI Hi s O31u Ar g As p 255 Pr o O31u O31y O31u Ar g 335 Tr p A a Ser O31u Th r 415 Le u Lys Arg Leu 445 Thr Leu N/t Page 482 ene Sequence 24- 2- 06. txt Leu Arg Asn 450 Pr o Val O31n 465 (3 y Lys Lys Val Ar g Asp Ser Tyr Leu 515 Pro O31n O31u 530 O3 y Leu Thr 545 O3 u Thr Thr Cys Arg Asp O31u Val Ala 595 Asp Trp Leu 610 Val Thr Ser 625 Pr o Thr Lys Asp Tyr A a Lys Val Ser 675 Leu 03 u Val 690 Phe 03 n A a 705 O31u Asp Pr o A a A a Lys Ar g Val Val 755 Leu Il e Thr 770 <210> 375 <211> 941 <212> DNA O31u ValI vb t 485 Ph e Ser Ser Ser Tr p 565 Le u Le u Le u Ar g O31n 645 Tr p Le u ValI A a I Ie 725 Ser As n Le u Leu Arg Ser Ilie Leu Leu Asn Ou Ou Arg 455 A a O31u Ph e A a Thr 535 Ser I Ie Ser Tyr Ar g 615 ValI O31u O31u I Ie ValI 695 Ph e ValI As p Ar g As n 775 Ly s ValI Pr o 505 ValI G31y G31y Thr A a 585 Ar g Ser As p I Ie I Ie 665 O31u Ser A a Thr Pr o 745 Le u A a Hi s 490 As p Tr p vb t Tr p ValI 570 Le u As p Tr p Tyr A a 650 Ly s Ser G31y Le u Pr o 730 G31y ValI Hi s 475 Ph e Tyr Le u Ly s Tr p 555 O31u Tyr Ly s Thr Thr 635 O31n A a A a Ser O31y 715 Ar g As n Ser Hi s As p I Ie As n 525 ValI O31u O31n Le u O31y 605 Le u O31n A a Tr p Thr 685 As n As n As p O31y A a 765 As p O31n As n 510 Pr o vb t vb t As p Le u 590 li1e Ser Tyr O31u A a 670 A a As p O31y Oy le 750 O31u vb t A a 495 Le u ValI As n Pr o Le u 575 G u Pr o Pr o Tyr A a 655 0Oy O n As p As p A a 735 Th r Th r Page 483 Gene Sequence 24-2-06.txt <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> FRXA02100 <400> 375 att gct ggt aag gca cac cca cat gac atg ggt ggc aag aag ctc atg 48 lie Ala G y Lys Ala His Pro His Asp IVet G y G y Lys Lys Leu IVet 1 5 10 cag gaa atc gtc cac ttc gct gat caa gct ggt gtc cgt gac cgt ttc 96 G n G u Ile Val His Phe Ala Asp G n Ala G y Val Arg Asp Arg Phe 25 ctc ttc ctg cct gat tac gac atc aac ctg gcc agc tac ctg atc tct 144 Leu Phe Leu Pro Asp Tyr Asp Ile Asn Leu Ala Ser Tyr Leu Ile Ser 40 ggt gct gac gtg tgg ctg aac aac cca gtg cgc cct cag gaa gca tcg 192 Sy Ala Asp Val Trp Leu Asn Asn Pro Val Arg Pro n G u Ala Ser 55 gga acc tcc ggt atg aag gcc gtc atg aat ggt ggc ctg acc ctg tcc 240 G y Thr Ser G y IVet Lys Al a Val IVet Asn G y G y Leu Thr Leu Ser 70 75 atc tct gat ggt tgg tgg gat gaa atg cct aag gag acc acc ggc tgg 288 I e Ser Asp G y Trp Trp Asp G u Iet Pro Lys G u Thr Thr G y Trp 90 acc atc cca acc gtt gag tcc cag gac ttg gaa tgc cgc gac cac ctg 336 Thr Ile Pro Thr Val u Ser G n Asp Leu G u Cys Arg Asp His Leu 100 105 110 gaa tcc cag gcg ctg tac gac ctg ctg gaa aac gaa gtt gca ccg ctg 384 Su Ser G n Ala Leu Tyr Asp Leu Leu G u Asn G u Val Ala Pro Leu 115 120 125 ttt tac aag cgc gac aag aac ggc atc cca cag gac tgg ctg gac ctg 432 Phe Tyr Lys Arg Asp Lys Asn y lie Pro G n Asp Trp Leu Asp Leu 130 135 140 gtt cgc gaa tcc tgg acc acc ctg tca cca atg gtc acc tcc acc cgc 480 Val Arg u Ser Trp Thr Thr Leu Ser Pro IVet Val Thr Ser Thr Arg 145 150 155 160 atg gtg cgc gac tac acc acc cag tac tac cgc cca acc aaa cac cag 528 IVet Val Arg Asp Tyr Thr Thr G n Tyr Tyr Arg Pro Thr Lys His G n 165 170 175 gca gag ctc att gcg cag cct gca gaa gca gcg gat tac gcg gca tgg 576 Ala G u Leu Ile Ala G n Pro Ala G u Ala Ala Asp Tyr Ala Ala Trp 180 185 190 ctt gag cac atc aaa gca gag tgg gct ggc gtc aag gtc tca gac ctg 624 Leu G u His lie Lys Al a G u Trp Ala G y Val Lys Val Ser Asp Leu 195 200 205 aag atc agc gag agc gcc atc acg gcg cag gag ctt gaa gtc agc gtt 672 Lys Ile Ser u Ser Ala lie Thr Ala n u Leu u Val Ser Val 210 215 220 cgc gtt gat tcc ggt tcg ctt aac gac gac gag ttc caa gct cag gca 720 Arg Val Asp Ser y Ser Leu Asn Asp Asp G u Phe G n Ala n Al a 225 230 235 240 Page 484 Gene Sequence 24- 2- 06. txt ctc ttt ggt gcg ctc gga cac aac ggt gac atc gaa gat cca gaa atc Leu Phe G y Ala Leu G y His Asn G y Asp I I e u Asp Pro G u lie 245 250 255 acc gtt ttg acc cca cgc ggc gat ggc gcc tac gcg gca aag gtc agc Thr Val Leu Thr Pro Arg G y Asp G y Al a Tyr Al a Al a Lys Val Ser 260 265 270 act gac ctg cca ggc aac tac ggc atc act gcc cgc gtt gtt cca aac Thr Asp Leu Pro G y Asn Tyr G y IIe Thr Ala Arg Val Val Pro Asn 275 280 285 aac agg atg ctg gtc agc cca gcg gaa acc cgc ctg atc acc tac ttg Asn Arg IVet Leu Val Ser Pro Ala G u Thr Arg Leu Ile Thr Tyr Leu 290 295 300 gag aac tagggcgaaa ctagctttac caa Su Asn 305 <210> 376 <211> 306 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 376 I e Al a G y Lys Ala Hi s 1 G n Leu Gy Gy I le Thr G u Phe Val 145 Met Al a Leu Lys SIle Leu Asp Ser Asp Pr o G n 115 Lys G u Ar g Leu Hi s 195 Ser 5 Hi s Asp Tr p et Tr p Val Leu Asp Tr p Tyr 165 Al a Lys Ser Phe Tyr Leu Lys 70 Tr p G u Tyr Lys Thr 150 Thr G n Al a Al a Pr o Al a Asp Asn Al a Asp Ser Asp Asn 135 Thr Thr Pr o G u l Ie 215 Hi s Asp SIle 40 Asn Val G u G n Leu 120 Gy Leu G n Al a Tr p 200 Thr Asp G n Asn Pr o bet et Asp 105 Leu SIle Ser Tyr G u 185 Al a Al a bet 10 Al a Leu Val Asn Pr o 90 Leu G u Pr o Pr o Tyr 170 Al a Gy Gy Gy Al a Ar g Gy Lys G u Asn G n et 155 Ar g Al a Val Lys Ar g Tyr G n Leu Thr Ar g Val 125 Tr p Thr Thr Tyr Val 205 Lys Asp Leu G u Thr Thr Asp 110 Al a Leu Ser Lys Al a 190 Ser Leu Ar g SIe Al a Leu Gy Hi s Pr o Asp Thr Hi s 175 Al a Asp Sn G u Leu G u Val Ser Val 220 Page 485 Arg Val Asp Ser y Ser Leu Asn A 225 230 Leu Phe G y Al a Leu G y Hi s Asn C 245 Thr Val Leu Thr Pro Arg Gy Asp C 260 2 Thr Asp Leu Pro y Asn Tyr y I 275 280 Asn Arg IVet Leu Val Ser Pro Ala C 290 295 Gu Asn 305 <210> 377 <211> 1206 <212> DNA <213> Cor ynebact eri um gl ut ai cum Sequence 24-2-06. t xt ,sp Asp u Phe n Al a n Ala 235 240 Sy Asp Ile G u Asp Pro G u lie 250 255 3y Ala Tyr Ala Ala Lys Val Ser 65 270 le Thr Ala Arg Val Val Pro Asn 285 Su Thr Arg Leu Ile Thr Tyr Leu 300 <220> <221> CDS <222> (1)..(1206) <223> FRXA02113 <400> 377 ct a ggt cga Leu G y Ar g 1 gac aag ctg Asp Lys Leu gat cca ct c Asp Pro Leu ct g cca at c Leu Pro Ile aag t ct gca Lys Ser Al a tac acc cac Tyr Thr Hi s cag gaa gag Gn u u gt t aaa gat Val Lys Asp 115 ggt gcg cag G y Al a n 130 aac Asn 5 t ac Tyr gcg Al a tct Ser gac Asp t ac Tyr aag Lys aac Asn gt a Val gcc Al a cag G n tac Tyr ggc G y tt g Leu 70 tt c Phe tac Tyr ggc G y aag Lys gag G u gac Asp ttc Phe gga G y 55 ggt G y acc Thr cac Hi s gag G u att SIle 135 gag G u acc Thr t cc Ser 40 ctt Leu gt g Val cag G n gat Asp cag G n 120 gca Al a caa G n gca Al a at g Met ggt G y cca Pr o tca Ser cca Pr o 105 gt c Val ct g Leu ct c Leu gca Al a ttt Phe ctt Leu at c SIle 75 t cc Ser gaa G u gtt Val gt a Val agc Ser acc Thr ggc G y gcg Al a ggt G y ggt G y ct g Leu tct Ser gca Al a 140 gaa G u gat Asp att SIle ggc G y gtt Val gac Asp ccg Pr o gt c Val 125 aac Asn t ac Tyr gct Al a cac Hi s gag G u ggt G y ggt G y att SIle 110 acc Thr gtt Val ct c Leu gt c Val cca Pr o aac Asn ttg Leu tgg Tr p gag G u tac Tyr ggc G y agc Ser gga G y agc Ser at g Met ct c Leu cag G n gca Al a cca Pr o cgc Ar g atc cca ttg ctg ctg ctt gat acc aac atc gag gca aac cca gaa gag Page 486 Gene Sequence 24- 2- 06. txt Thr Asn lie u Ala Asn Pro u u lie Pro Leu Leu Leu Leu Asp 145 150 ct c Leu at c SIle gca Al a aac Asn at c SIle 225 t cc Ser ttc Phe gat Asp ggt Sy cgc Ar g 305 agc Ser gt g Val aag Lys ctt Leu aag Lys 385 gct Al a aac Asn cag G n tgc Cys 195 ggc Gy gag G u at c SIle at g bet cag G n 275 gag G u agc Ser gac Asp at c SIle gag G u 355 gtt Val tgg Tr p act Thr 165 ct c Leu gct Al a gca Al a at g bet acc Thr 245 at g et tgc Cys gat Asp cat Hi s ttc Phe 325 cac Hi s aag Lys gat Asp gt g Val gac Asp gtt Val cgt Ar g ggt Gy gag G u 230 acc Thr gt g Val gtt Val cca Pr o gct Al a 310 gcc Al a gt c Val gaa G u tct Ser cgc Ar g 390 cgc Ar g ct c Leu ggt Gy ttc Phe 215 t ac Tyr cac Hi s cgt Ar g ggt G y cac Hi s 295 aat Asn ggc G y acc Thr ct c Leu tgg Tr p 375 aac Asn ct g Leu ggt Gy ct g Leu 200 ct g Leu cca Pr o acc Thr cgt Ar g gtt Val 280 cgc Ar g ggc G y ct g Leu aac Asn at c SIle 360 t ca Ser aag Lys t ac Tyr gtt Val 185 aag Lys acc Thr gca Al a cca Pr o t at Tyr 265 cca Pr o ttc Phe gt c Val t ac Tyr ggt Gy 345 gat Asp aac Asn ttc Phe ggt G y 170 ggt Gy cgc Ar g ct g Leu gca Al a gt c Val 250 ct c Leu at t SIle aac Asn gca Al a ccc Pr o 330 gtt Val cgc Ar g cca Pr o cgt Ar g 155 ggc G y ggc Gy t ca Ser gag G u ttc Phe 235 cca Pr o ggt G y gag G u at g bet aag Lys 315 gga G y cac Hi s gt c Val cag G n gct Al a 395 gac Asp gt c Val tct Ser cgt Ar g 220 gag G u gca Al a ggc G y aag Lys gct Al a 300 ctt Leu t at Tyr ct g Leu act Thr gct Al a 380 gac Asp aat Asn cgc Ar g gtt Val 205 at c SIle cag G n ggc G y ggt G y gca Al a 285 cat Hi s cat Hi s gag G u ccg Pr o ggc G y 365 gt c Val ct a Leu gag G u gct Al a 190 gca Al a cgc Ar g gtt Val at c SIle cag G n 270 ctt Leu at g bet ggt G y cct Pr o acg Thr 350 ggc G y gag G u gt g Val cac Hi s 175 gt c Val cac Hi s gag G u cgt Ar g gac Asp 255 cca Pr o gag G u ggc G y gaa G u cgt Ar g 335 tgg Tr p gct Al a t ct Ser gag G u 528 576 624 672 720 768 816 864 912 960 1008 1056 1104 1152 1200 1206 Page 487 Gene Sequence 24-2-06. t xt <210> 378 <211> 402 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 378 Leu G y Ar g 1 Asp Lys Leu Asp Pro Leu Leu Pro Ile Lys Ser Al a Tyr Thr Hi s Gn Gu u Val Lys Asp 115 G y Al a G n 130 Ile Pro Leu 145 Leu Arg Asn Ile Lys G n Ala Phe Cys 195 Asn G u y 210 Ile Ala G u 225 Ser Asn Ile Phe Asp Mbet Asp n n 275 Sy n u 290 Ar g Al a Ser 305 Ser Arg Asp lie Asn Ala G u G u n 5 Tyr Al a Ser Asp Tyr Lys Asn Val Leu Thr 165 Leu Al a Al a bet Thr 245 et Cys Asp Hi s Phe 325 G n Ty r Sy Leu 70 Phe Ty r Sy Lys Leu 150 Asp Val Ar g Sy G u 230 Thr Val Val Pr o Al a 310 Al a Asp Phe Sy 55 Sy Thr Hi s G u SIle 135 Asp Ar g Leu Sy Phe 215 Tyr Hi s Ar g Sy Hi s 295 Asn Gy Thr Ser 40 Leu Val G n Asp G n 120 Al a Thr Leu Sy Leu 200 Leu Pr o Thr Ar g Val 280 Ar g Sy Leu Al a bet Sy Pr o Ser Pr o 105 Val Leu Asn Tyr Val 185 Lys Thr Al a Pr o Tyr 265 Pr o Phe Val Tyr Asn 10 Asp G u Val Leu Leu 90 Al a Thr Tr p SIle Sy 170 Sy Ar g Leu Al a Val 250 Leu SIle Asn Al a Leu Al a Phe Leu SIle 75 Ser G u Val Val G u 155 Sy Sy Ser G u Phe 235 Pr o Sy G u et Lys 315 Ser Thr Sy Al a Sy Sy Leu Ser Al a 140 Al a Asp Val Ser Ar g 220 G u Al a Sy Lys Al a 300 Leu G u Asp SIle Sy Val Asp Pr o Val 125 Asn Asn Asn Ar g Val 205 I le G n Sy Sy Al a 285 Hi s Hi s Tyr Al a Hi s G u Sy Sy SIle 110 Thr Val Pr o G u Al a 190 Al a Ar g Val SIle G n 270 Leu bet Sy Leu Val Pr o Asn Leu Tr p G u Tyr Sy G u Hi s 175 Val Hi s G u Ar g Asp 255 Pr o G u Sy G u Ser Sy Ser vet Leu G n Al a Pr o Ar g G u 160 Ar g Asn Leu Ar g Al a 240 Ar g G u Leu Leu Val 320 Pro Gy Tyr G u Pro 330 Page 488 Arg G u 335 Gene Sequence 24- 2- 06. txt Val Pro lie Gy His Val Thr Asn G y Val His Leu Pro Thr Trp Val 340 345 350 Lys Pro G u Iet Lys G u Leu I e Asp Arg Val Thr G y G y Al a Asp 355 360 365 Leu Ala Val Ala Asp Ser Trp Ser Asn Pro G n Ala Val u Ser G u 370 375 380 Lys lie Trp Lys Val Arg Asn Lys Phe Arg Ala Asp Leu Val G u Val 385 390 395 400 Al a Ar g <210> 379 <211> 1140 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1117) <223> RXA02147 <400> 379 aaaagttgag agcggcttgc ttcttttcct tggctaggct gtggagttgc ttgaatgagg ttgatagggg atttttgaag tttgtaatcg ggt t agagta ttt ggt cgc Phe G y Ar g tgg Tr p gt g Val at c l Ie cag G n cag G n aat Asn t at Tyr gat Asp aag Lys agc Ser tcg Ser cat Hi s gag G u cag G n gaa G u ggc Sy caa G n 120 act Thr gt g Val t cc Ser tct Ser gat Asp gt a Val t at Tyr gt c Val gt g Val gat Asp gcg Al a ggt G y cag G n at t SIle agc Ser 75 cgt Ar g acg Thr at t SIle gt g Val t ca Ser gcg Al a gaa G u gag G u t ac Tyr tcg Ser gat Asp gat Asp gtt Val tgt gtt Cys Val gag gaa G u G u acg tct Thr Ser 45 gct cgt Al a Ar g cgt gag Arg G u gag at c G u Ile cct ttg Pro Leu 110 cgg atg Arg IVet 125 gaa t cc Su Ser gca Al a gat Asp cag G n gt c Val gct Al a 80 cgg Ar g at t SIle t ac Tyr acg Thr ct g Leu gag G u at c SIle caa G n gct Al a gt g Val t ca Ser 130 at t I e gct Al a gt c Val gaa G u tcg Ser gcg Al a 100 gca Al a ttg Leu cgt Ar g ctg Leu gat Asp aaa Lys gtt Val gag G u aag Lys gaa G u act Thr ctc aac gcg gag act gag aag Leu Asn Ala G u Thr G u Lys Page 489 Gene Sequence 24-2-06.txt t cc Ser 150 ttc Phe aag Lys gcc Al a ttg Leu ggt Gy 230 ggt Gy gcg Al a at g Met gt c Val gac Asp 310 gt a Val gaa G u ttg Leu gca Al a acg Thr 200 at t SIle gt g Val at t SIle cag G n ggc Gy 280 gga Gy aag Lys gtt Val gt g Val cag G n 170 gat Asp cag G n ct g Leu gct Al a cca Pr o 250 at g Net acg Thr t ac Tyr gt g Val t ca Ser 330 t at Ty r 155 ct g Leu ggt Sy gag G u act Thr gcc Al a 235 aat Asn ggt G y ccg Pr o cca Pr o cac Hi s 315 gca Al a caa G n aag Lys cga Ar g cgg Ar g gat Asp 220 ttg Leu gag G u aag Lys gt g Val ggt G y 300 gcc Al a ccg Pr o gca Al a gt a Val aaa Lys gaa G u 205 ttg Leu tct Ser ttt Phe act Thr agc Ser 285 gct Al a t ca Ser ttt Phe aac Asn cgc Ar g tcg Ser 190 at g et ctt Leu aag Lys gat Asp ttg Leu 270 aga Ar g act Thr gac Asp t at Tyr cgt Ar g cag G n 175 gag G u tgg Tr p ggt Gy ttg Leu tgc Cys 255 cca Pr o gat Asp cac Hi s t ac Tyr ggt Gy 335 act Thr 160 gcg Al a at c SIle gtt Val ctt Leu gga Gy 240 t ca Ser cgt Ar g gag G u gt g Val gga Gy 320 gcg Al a 145 aag Lys gag G u cga Ar g gct Al a t cc Ser 225 agc Ser ggt Gy acg Thr ct g Leu gga Gy 305 at c SIle cgt Ar g gcg Al a ctt Leu gac Asp aag Lys 210 gct Al a cct Pr o ttg Leu tct Ser cag G n 290 ct g Leu cct Pr o cgc Ar g gaa G u gaa G u cgg Ar g 195 aat Asn gcg Al a t at Tyr at c SIle caa G n 275 cct Pr o t at Tyr gt g Val t ac Tyr gcg Al a tct Ser 180 gt g Val ggt Sy act Thr ggt Sy t at Tyr 260 gct Al a ggc G y at t SIle cag G n gag G u 165 gaa G u gat Asp cca Pr o tcg Ser tgg Tr p 245 tgg Tr p cag G n gat Asp ggg G y gt g Val 325 595 643 691 739 787 835 883 931 979 1027 1075 1117 1140 taagaaatag ttcgtcagga gaa <210> 380 <211> 339 <212> PRT <213> Corynebacter i um gl utam cum <400> 380 lIet Phe y Arg Arg Trp Val Ser Val Val Ala Ser Cys Val I e Ala 1 5 10 Ser Thr Leu lie Leu Val Pro Ser His Ser y Ala G u G u Val Asp 25 Sn Leu Ile Ala Asp Ile u His Val Ser n G u Thr Ser Ala n 40 Page 490 Asn G u G u Val Lys G n Leu 55 Gene Sequence 24-2-06.txt Gu Ile Asp Ile u Ala Arg u Val Thr Asp SIle Al a Leu Al a 145 Lys G u Ar g Al a Ser 225 Ser Gy Thr Leu Gy 305 I le Ar g Lys Al a G n Ser 115 Thr Thr G u G u Ar g 195 Asn Al a Tyr SIle G n 275 Pr o Tyr Val Tyr Val G u Lys G u Thr Lys G u 165 G u Asp Pr o Ser Tr p 245 Tr p G n Asp Gy G u Val Ar g Pr o Ser 135 Al a G n G u Leu Asp 215 Al a Gy Tyr Al a SIle 295 Gy G n G u Sy G n 120 Thr G u Leu Al a Thr 200 I le Val SIle G n Sy 280 Sy Lys Ser Al a Thr 105 Asn Ser Al a Sy Leu 185 Pr o Asp Asp Sy G n 265 Sy Tyr SIle Ser Ar g Thr SIle Val Tyr 155 Leu Sy G u Thr Al a 235 Asn Sy Pr o Pr o Hi s 315 Ar g G u Pr o Ar g 125 G u Al a Val Lys G u 205 Leu Ser Phe Thr Ser 285 Al a Ser Al a Asn Ser Ser Leu Ar g G n 175 G u Tr p Sy Leu Cys 255 Pr o Asp Hi s Tyr G n Val Val Ser Val Asp Ser Ala Pro Phe Tyr 325 330 G y Al a 335 <210> 381 <211> 1959 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1936) <223> RXA01478 Page 491 ene Sequence 24- 2- 06. txt <400> 381 gcgggttttg ttgtggaggg gcgcgtcgaa aagcaatttt t at tggagt c acct ggcct g aaat cctct a ctct gggcgc t t t t ccaaga t agct cact t at g acc at t cca l'bt Th r I Ie Pro gct A a gcg A a agt Ser ac c Th r cca Pr o gt g ValI t tc Ph e cgc Ar g cgc Ar g act Th r 1 50 gt a ValI c ac Hi s ct c Le u ac c Thr gag O31u 230 ac a Thr ct g Le u g ac As p ct g Le u ggt G31y ac c Thr cca Pr o gt g ValI 120 cgt Ar g g ac As p gt t ValI ggc G31y t gg Tr p 200 t ct Ser ccc Pr o act Thr g ac As p t tg Le u ggt G31y gt g ValI t gg Tr p c ac Hi s t gc cys g at As p at c I Ie gga G31y 1 70 tt c Ph e ccg Pr a gag O31u c ag O31n at c I Ie c ac Hi s ct g Le u cgc Ar g ag c Ser t ca Ser c aa O31n g aa O31u G31y 140 at c I Ie gag O31u ct g Le u tt c Ph e ac c Thr 220 ct c Le u ct g Le u ggc G31y cgt Ar g c ac Hi s aac As n ac c Thr c aa O31n 110 g aa (31u t cc Ser c ag O31n cca Pr a gcc A a 1 ccg Pr a ag c Ser g ac As p g ac As p 15 gct A a tt t Ph e gga O31y t ac Tyr ggt O31y 95 ccc Pr a gt g ValI act Thr gct A a c ac Hi s 1 75 ggc O31y cat Hi s tt c Ph e gct A a ac a Thr ct g Le u g at As p c ac Hi s ct c Le u 80 act Thr g at As p g at As p ccg Pr a gt c ValI 1 60 cgc Ar g g aa O31u ccc Pr a t gg Tr p g aa O31u 240 1 ct t Le u ct g Le u t cc Ser t gg Tr p ggc O31y gcc A a at c I Ie gt g ValI t at Tyr 145 gcc A a cct Pr a t cc Ser ccc Pr a gca A a 225 gt c ValI ct t Le u t cc Ser c aa O31n agt Ser g at As p cgg Ar g ac c Thr g aa O31u 130 tt c Ph e ggc O31y gca A a gt g ValI at g vb t 210 t ca Ser cgc Ar g g at As p 20 at g vb t at g vb t cgt Ar g tt c Ph e gt t ValI 1 00 ct g Le u at c I Ie ac c Thr aat As n g at As p 1 t gg Tr p g at As p gt t ValI t cc Ser gga O31y t ac Tyr O31y tt c Ph e gt c ValI gt g ValI g at As p gt g ValI ct g Le u ag c Ser gcg A a 1 t gt cys gt t ValI t ac Tyr g aa O31u at g vb t 245 ct c gt a ct g cgc gcc t t g acc gat ct a caa acc ggt ggc at c gt g gcc Page 492 Leu Val Leu Arg Ala Leu Thr 250 Gene Sequence 24-2-06.txt Asp Leu Gn Thr G y Gy I le Val Al a 260 gca Al a gac Asp ct c Leu ct g Leu 310 ggc G y cgc Ar g gaa G u acc Thr caa G n 390 caa G n cgc Ar g gaa G u aaa Lys t cc Ser 470 cag G n agc Ser acc Thr cgc Ar g 280 gaa G u cgc Ar g ggc G y t ac Tyr t ac Tyr 360 cgc Ar g gcc Al a at c SIle at g Met aac Asn 440 cgc Ar g acc Thr gcc Al a gt a Val t ca Ser gt g Val gga G y at c SIle gaa G u 330 aac Asn gca Al a gcc Al a ct c Leu gaa G u 410 tgg Tr p gac Asp gaa G u tgc Cys at a l Ie 490 cgc Ar g ct a Leu tgg Tr p tt c Phe gca Al a 315 cga Ar g t cc Ser gat Asp ggg G y gat Asp 395 at g bet gcc Al a ggc G y at c SIle tac Ty r 475 ggc G y at t SIle cca Pr o ct g Leu t cc Ser 300 ggc G y cac Hi s gt g Val gt c Val tgc Cys 380 ttc Phe cgc Ar g ggc G y ccc Pr o at g Net 460 gac Asp ttc Phe gag G u gag G u cgc Ar g 285 caa G n gac Asp ct c Leu cct Pr o gt c Val 365 ct c Leu caa G n t cc Ser ttc Phe at c SIle 445 acc Thr aac Asn at c SIle caa G n gat Asp 270 gac Asp gca Al a ccg Pr o cct Pr o gtt Val 350 ggc G y gag G u gaa gaa G u gac Asp 430 gag G u aac Asn acc Thr ggc G y gag G u 510 255 ttc Phe t cc Ser gcc Al a gaa G u gaa G u 335 cgc Ar g gaa G u gac Asp gcc Al a ccg Pr o 415 cgc Ar g cgc Ar g ggc G y caa G n ttc Phe 495 ctt Leu gga G y gca Al a ct c Leu aac Asn 320 cgc Ar g gtt Val gt a Val gaa G u aac Asn 400 caa G n ggc G y tgg Tr p ttc Phe gt c Val 480 gac Asp ct c Leu ggc G y ct c Leu caa G n 305 ct c Leu gaa G u ggc G y at g bet ttc Phe 385 ttc Phe t at Tyr at c SIle cgt Ar g aac Asn 465 gac Asp gat Asp gac Asp at c SIle acc Thr 290 tgg Tr p cgc Ar g ct c Leu aat Asn gt c Val 370 t cc Ser gac Asp ttc Phe aaa Lys gaa G u 450 gaa G u gcc Al a cca Pr o gcc Al a cgt Ar g 275 at t SIle cgc Ar g at c SIle caa G n gga G y 355 gcg Al a tgg Tr p cgc Ar g acc Thr gcc Al a 435 ct c Leu gag G u tcg Ser aaa Lys cac Hi s 515 aac Asn gaa G u acc Thr at g Net cac Hi s 340 gcc Al a ctt Leu ggc G y aag Lys cac Hi s 420 at c l Ie cgc Ar g at c SIle ct g Leu at g Net 500 ggc G y tgg Tr p gcc Al a tgg Tr p t at Tyr 325 ct g Leu gcc Al a gaa G u at g let gat Asp 405 ggc G y gaa G u gcc Al a caa G n ctt Leu 485 ct c Leu ttt Phe 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 ctt cac agg tac cac acc gac ggg tct gac ggc ctt gcc ggc gac gaa Page 493 Leu His Arg Tyr His Thr Asp 520 tac ccc ttc ctc atc tgt tca Tyr Pro Phe Leu lie Cys Ser 535 540 tcc aac cgc ctc gac gag gcc Ser Asn Arg Leu Asp G u Ala 550 555 gtc caa agc cca ctt ggc cta Val G n Ser Pro Leu G y Leu 570 ggc agg ctc gct gga aac tac G y Arg Leu Ala G y Asn Tyr 585 atc agc gct gcc cgt gcc ata Ile Ser Ala Ala Arg Ala lle 600 tagagtctaa ggtgtcat t c ttg <210> 382 <211> 612 <212> PRT Gene Sequence 24-2-06.txt G y Ser Asp G y Leu Al a G y 525 530 ttc tgg ctg gta gaa caa tac Phe Trp Leu Val G u G n Tyr 545 aaa gaa aag atg aac cgc atc Lys G u Lys IVet Asn Arg Ile 560 ctg gct gag gaa tac tcc acc Leu Ala G u G u Tyr Ser Thr 575 cct cag gcc ttt tcc cac att Pro G n Ala Phe Ser His lie 590 595 aat ttc gaa gaa gcg cga aac Asn Phe G u G u Ala Arg Asn 605 610 Asp gca Al a ctt Leu cac Hi s 580 ggt Gy agg Ar g G u agc Ser gcc Al a 565 cat Hi s ct g Leu 1747 1795 1843 1891 1936 1959 <213> Corynebacter i um gl ut ai cum <400> 382 Iet Thr Ile 1 Leu Leu Ser Tr p Gy Al a SIle Val Tyr 145 Al a Pr o G u Asn Al a Leu Ser Val Asp 115 Ser Ar g Pr o Lys Pro G y Al a 5 Asp Tyr Al a [et y Ser Net Phe Thr Arg Val Pro 70 Phe Val Val Val Asp Phe 100 Leu Val Ar g I e Leu Ar g Thr Ser Thr 150 Asn Al a Val 165 Asp Cys Hi s 180 Ser Leu Leu Ar g Gy G n bet Ser Leu 135 Val Tyr Ser G n Ser Tr p Leu G u Val SI e 105 Hi s Phe Gy Ar g Thr 185 Asp Ile Pro Thr Hi s Thr Cys Leu Pro Asp Arg u Ile Ser G n Arg Ser G u Sy n u Val u y 125 Tyr G y G u 140 Ser Ile Val 155 Pro G u Nbet Hi s Leu Thr Asp Al a Phe Gy Tyr Gy Pr o Val Thr Al a Hi s 175 Gy Page 494 Gene Sequence 24-2-06.txt Ala Pro Ser Phe G u Pro Ser Val G u Trp Val Leu Thr 195 Pr o Al a 225 Val Sy Sy Leu G n 305 Leu G u Sy et Phe 385 Phe Tyr SIle Ar g Asn 465 Asp Asp Asp Leu G u 545 Pr o Tr p Ar g SIle Ar g 275 I le Ar g SIle G n Sy 355 Al a Tr p Ar g Thr Al a 435 Leu G u Ser Lys Hi s 515 Sy Tyr Tyr G u let 245 Al a Tr p Al a Tr p Tyr 325 Leu Al a G u let Asp 405 Sy G u Al a G n Leu 485 Leu Phe G u Ser Ar g 215 Leu Val Pr o Tyr Val 295 Leu Leu Gy G n SIle 375 Lys Sy Al a Phe Leu 455 Phe Leu Thr Hi s Pr o 535 Asn Tr p 200 Ser Pr o Leu Thr Ar g 280 G u Ar g Sy Tyr Tyr 360 Ar g Al a SIle let Asn 440 Ar g Thr Al a Val Ar g 520 Phe Ar g His Pro 205 Leu Hi s Ar g Thr 265 Tyr Tyr Al a Sy G u 345 G n Ar g l Ie Tr p let 425 Leu G u G n G n Al a 505 Tyr Leu Leu G u G n Al a 250 Ser Val Sy l Ie G u 330 Asn Al a Al a Leu G u 410 Tr p Asp G u Cys SIle 490 Ar g Hi s SIle Ser Ar g 235 Leu Leu Tr p Phe Al a 315 Ar g Ser Asp Sy Asp 395 let Al a Sy SIle Tyr 475 Sy SIle Thr Cys Leu Tyr Asp G u Ar g 285 G n Asp Leu Pr o Val 365 Leu G n Ser Phe SIle 445 Thr Asn SIle G n Sy 525 Phe Phe Al a G n 255 Phe Ser Al a G u G u 335 Ar g G u Asp Al a Pr o 415 Ar g Ar g Sy G n Phe 495 Leu Asp Leu Asp u Ala Lys u Lys Iet 555 560 Page 495 Gene Sequence 24- 2- 06. txt Asn Arg lie Leu Ala Val On Ser Pro Leu Gy Leu Leu Ala G u u 565 570 575 Tyr Ser Thr His His G y Arg Leu Ala G y Asn Tyr Pro G n Ala Phe 580 585 590 Ser His IIe G y Leu lie Ser Ala Ala Arg Ala lie Asn Phe G u G u 595 600 605 Ala Arg Asn Arg 610 <210> 383 <211> 658 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(658) <223> RXA01888 <400> 383 agtagatact agataccacc cat t gatgcc gtcaaggggt ttcctgtaaa gatgtaagag attaagaaaa gaggtagata tggcgtcaaa gcgaccgaca atg gct gat gtg gca Met Ala Asp Val Ala aaa Lys gcc Al a gcc Al a aac Asn cac Hi s ttc Phe gcc Al a gga Gy ccc Pr o gct Al a gga G y ct c Leu t cc Ser gat Asp ct g Leu aac Asn cgc Ar g 120 caa G n gt a Val agt Ser t ac Tyr ct c Leu gt c Val ctt Leu ct g Leu t ca Ser at t SIle t cc Ser gaa G u at t SIle at c SIle gaa G u 75 agc Ser at c SIle cct Pr o gcc Al a act Thr t cc Ser cct Pr o ggt G y cac Hi s gcg Al a agg Ar g agt Ser cgc Ar g 140 gcg Al a acc Thr gat Asp 45 gt g Val ct c Leu at c l Ie gaa G u gat Asp 125 ggt Gy ct g Leu cgc Ar g cga Ar g gca Al a t at Tyr aca Thr cga Ar g 110 ttg Leu t cc Ser gt c Val 15 aac Asn cga Ar g ttc Phe ccc Pr o ccg Pr o 95 tgc Cys gaa gau gga Gy t cc Ser cat Hi s gcc Al a gaa G u acc Thr 80 act Thr gaa G u aca Thr acc Thr 1 at c SIle gt g Val caa G n at g bet gcc Al a cgc Ar g gca Al a at c SIle ccc Pr o 145 gt g Val aaa Lys aaa Lys cac Hi s cga Ar g act Thr gt a Val gca Al a 130 aaa Lys ttt Phe gaa G u ctt Leu caa G n aaa Lys gaa G u at t SIle 115 cag G n gt c Val cgc Ar g aaa Lys cgc Ar g gca Al a cat Hi s aaa Lys 100 ctt Leu caa G n agt Ser gat Asp gcc Al a caa G n ttc Phe ggc G y gat Asp ct a Leu ctt Leu t cc Ser gtc cat gtc gac gac gca gtt ggc gcc caa tta gcc ctc aac cac ctc Page 496 Val Hi s Val 150 atc gaa tta I e G u Leu cct ggc acc Pro G y Thr Asp cac Hi s 170 gaa Gu Gene Sequence 24-2-06.txt Ala Val Gy Ala G n Leu Ala Leu Asn His Leu 155 160 165 gaa cac atc atc tac atc gat ggt ggc gac gcc Su His Ile lie Tyr Ile Asp Gy y Asp Ala 175 180 <210> 384 <211> 186 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 384 Met Ala Asp Val 1 I e Val G n et Al a Ar g Al a SIle Pr o 145 Al a Asp Phe G u Leu G n Lys G u l Ie 115 G n Val Asn Sy Al a Lys Al a 5 Asp Al a Pro Al a Al a G u Sn Asn Arg Phe Hi s G y 70 G y Phe Asp Asp Al a Val Leu G y Ser Leu Pro Val 135 Ser Val Hi s 150 Leu Il e G u 165 Al a Pro G y Sy Al a Sy Sy SIle Tyr Al a 105 bet Val Asp Sy G n 185 Ser G u I e I e G u 75 Ser l Ie Pr o Al a Al a 155 G u Al a Thr Asp Val Leu SIle G u Asp 125 G y Gy lie Leu Ar g Ar g Al a Tyr Thr Ar g 110 Leu Ser Al a SIle Val Asn Ar g Phe Pr o Pr o Cys G u Sy G n Tyr 175 Ser Hi s Al a G u Thr Thr G u Thr Thr Leu 160 I e <210> 385 <211> 1503 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1480) <223> RXN01927 <400> 385 gagcagcggg atcttttgcg taattcgcgc gcgcagatcc atgtgattga ccacaatggt Page 497 Gene Sequence 24- 2- 06. txt gatgaaattt tggatacccc aacggaagag gatttttaag atg gct t tg gtt ctt Leu Val Leu lMt Ala L gga Gy gcc Al a tcg Ser gag G u cac Hi s ttg Leu gag G u t at Tyr gaa G u ttc Phe 150 ggt Gy cgc Ar g gaa G u aaa Lys gat Asp 230 gat Asp ggc Gy gt a Val ttg Leu at g let tgg Tr p at c SIle gct Al a 120 gaa G u acc Thr gct Al a gat Asp ct g Leu 200 gct Al a cag G n t cc Ser gtt Val cca Pr o gaa G u gcg Al a gac Asp ggc Gy tta Leu gca Al a cat Hi s gga Gy 170 gct Al a cca Pr o ggc Gy ggt Gy caa G n gac Asp gcg Al a gcg Al a gat Asp cgt Ar g cag G n gcc Al a cgc Ar g 140 at g bet ggc Gy ttg Leu gcg Al a gga Gy 220 gt c Val t cc Ser gaa G u tgg Tr p 45 gac Asp gaa G u tct Ser gct Al a acc Thr 125 acg Thr gga Gy t ac Tyr gcg Al a at t l Ie 205 gat Asp agc Ser tgc Cys ggc Gy at c SIle gct Al a aac Asn gcc Al a gcg Al a 110 aaa Lys gcg Al a cgc Ar g t ac Tyr ct g Leu 190 gcg Al a aat Asn gt g Val aag Lys 15 cgc Ar g gct Al a gt a Val gat Asp cag G n 95 gt a Val at g bet tcg Ser gga Gy agc Ser 175 ggc Gy gga Gy gct Al a tcg Ser cac Hi s 255 gct Al a gcg Al a gcg Al a tct Ser gaa G u gct Al a gat Asp cgg Ar g gt g Val cgc Ar g 160 acg Thr cat Hi s aca Thr gcg Al a at c SIle 240 gat Asp ttg Leu agt Ser ct g Leu at t SIle at c SIle gcg Al a gcc Al a tgg Tr p at g Net 145 aaa Lys cgt Ar g gag G u act Thr gct Al a 225 ggc Gy cca Pr o ctt Leu cac Hi s gat Asp gca Al a gtt Val ttg Leu acg Thr at g Net 130 ttg Leu gt c Val gat Asp gt g Val cca Pr o 210 gcg Al a acc Thr tct Ser gac Asp agc Ser gct Al a cag G n ccg Pr o ct c Leu 100 agt Ser gat Asp cat Hi s gac Asp gcg Al a 180 ctt Leu gga gcy ggc Gy ggc Gy ttg Leu 260 gcc ggc atg acc gtt caa cat agc gtc Ala G y let Thr Val G n His Ser Val 250 Page 498 Gene Sequence 24- 2- 06. txt act ggt ttc gcc gat gcc acg ggt gcg tat ttc ccg ctg gcc tgc acg 931 Thr y Phe Ala Asp Al a Thr y Ala Tyr Phe Pro Leu Ala Cys Thr 265 270 275 ctt aat ggc gca ccg gtg ttg gaa ttc ggc cgc cgc att ctg ggc gtg 979 Leu Asn G y Al a Pro Val Leu G u Phe G y Arg Arg Ile Leu G y Val 280 285 290 gaa tgg gaa gag ttc gat gcg ctt gca ctg gct gct caa ccc ggt tca 1027 G u Trp G u G u Phe Asp Ala Leu Ala Leu Ala Ala G n Pro y Ser 295 300 305 ggt ggc gtg acg ctc cag cct tat ttg gag ggc gag cgt acg ccg aat 1075 Gy y Val Thr Leu G n Pro Tyr Leu u Gy u Arg Thr Pro Asn 310 315 320 325 cgt ccc gca gca cgt ggc gtt ttg gct gga cta aac tgt gca acg acc 1123 Arg Pro Ala Ala Arg G y Val Leu Ala y Leu Asn Cys Ala Thr Thr 330 335 340 cgc gag gac ttt gcc cga gca act gtt gaa ggc ttg ttg ttg gca ttg 1171 Arg G u Asp Phe Ala Arg Ala Thr Val G u y Leu Leu Leu Ala Leu 345 350 355 gat gat gct gta acg gcg ctg gtt gag gcc acg gga gtg ccc gtt cag 1219 Asp Asp Al a Val Thr Al a Leu Val G u Al a Thr G y Val Pro Val G n 360 365 370 cgc atc cag ctc atc ggt ggc ggc gcg cgt tca cag gcg gtt cgt gag 1267 Arg lle n Leu Ile y G y y Ala Arg Ser n Ala Val Arg u 375 380 385 att gcc cct gag att ttc ggc cat gag att gtg gtt cca gaa ccc gct 1315 IIe Ala Pro G u IIe Phe G y His G u Ile Val Val Pro G u Pro Ala 390 395 400 405 gaa tat gtg gcg ttg ggt gca gct cgt cag gcg gca tgg gcg ctg tcg 1363 G u Tyr Val Ala Leu G y Ala Ala Arg G n Ala Ala Trp Ala Leu Ser 410 415 420 ggt gag gcc acg cca ccg cag tgg cca act ccc ggt tcc gat ccg cac 1411 G y G u Ala Thr Pro Pro G n Trp Pro Thr Pro G y Ser Asp Pro His 425 430 435 cgc gca cct aaa aac act gag ctg agc acg cgt tat gcg aag ctg cgt 1459 Arg Ala Pro Lys Asn Thr G u Leu Ser Thr Arg Tyr Ala Lys Leu Arg 440 445 450 gct gca acg cag ggt tgg tac tagagctcga tattgtcgat caa 1503 Ala Ala Thr G n y Trp Tyr 455 460 <210> 386 <211> 460 <212> PRT <213> Corynebacter i um gl utam cum <400> 386 IVet Ala Leu Val Leu G y Ile Asp Ser Ser Thr G n Ser Cys Lys Ala 1 5 10 Leu Leu Val Asp Ala Ala Thr Oy n Val lie Asp u y Arg Ala 25 Ser His Pro Ser G y Ser G u Val Asp Pro Arg Ala Trp lie Ala Ala 40 Page 499 Leu Asp I e Al a Il e Val Al a Leu Al a Thr Trp lMet 130 lIet Leu 145 Lys Val Arg Asp G u Val Thr Pro 210 Al a Al a 225 G y Thr Pro Ser Pro Leu Arg Ile 290 Al a G n 305 G u Ar g Asn Cys Leu Leu G y Val 370 G n Al a 385 Val Pr o G n Ala Thr u Sy Ar g Asp Sy 115 Ar g Pr o Thr Ar g G u 195 Sy Leu Ser Sy Al a 275 Leu Pr o Thr Al a Leu 355 Pr o Val G u G n Al a Asn Val Hi s Asp Hi s 165 Tr p Pr o Val Leu Val 245 Val Thr Val Ser Asn 325 Thr Leu G n G u Al a 405 Hi s 70 Leu G u Tyr G u Phe 150 Sy Ar g G u Lys Asp 230 Al a Thr Leu G u Sy 310 Ar g Ar g Asp Ar g SIle 390 G u Sy 55 Sy Leu G u Val Pr o 135 Leu Asp Thr Leu Val 215 Leu Sy Sy Asn Tr p 295 Sy Pr o G u Asp SIle 375 Al a Tyr Gene Sequence 24-2-06.txt Leu Leu G u Arg Ala Asp Ala Val Ser let Tr p SIle Al a 120 G u Thr Al a Asp Leu 200 Al a G n let Phe Sy 280 G u Val Al a Asp Al a 360 G n Pr o Val Val Asn Sy 105 Ser Asn Tr p Ser Leu 185 Al a Al a Pr o Thr Al a 265 Al a G u Thr Al a Phe 345 Val Leu a u Al a Al a Asp 90 Sy Leu Al a Hi s Sy 170 Al a Pr o Sy Sy Val 250 Asp Pr o Phe Leu Ar g 330 Al a Thr SIle SIle Leu 75 Thr Asp Thr Al a Leu 155 Thr Al a Asn Thr Asp 235 G n Al a Val Asp G n 315 Sy Ar g Al a Sy Phe 395 G u Ser Al a Thr 125 Thr Sy Tyr Al a SIle 205 Asp Ser Ser Sy G u 285 Leu Tyr Leu Thr Val 365 G y Hi s Asn Al a Al a 110 Lys Al a Ar g Tyr Leu 190 Al a Asn Val Val Al a 270 Phe Al a Leu Al a Val 350 a u Al a G u Asp G n Val bet Ser Sy Ser 175 Sy Sy Al a Ser Hi s 255 Tyr Sy Leu G u Sy 335 G u Al a Ar g SIle G u Al a Asp Ar g Val Ar g 160 Thr Hi s Thr Al a SIe 240 Asp Phe Ar g Al a Sy 320 Leu Sy Thr Ser Val 400 Leu G y Ala Ala Arg 410 Page 500 G n Al a 415 Gene Sequence 24-2-06.txt Ala Trp Ala Leu Ser Gy u Ala Thr Pro Pro n Trp Pro Thr Pro 420 425 430 G y Ser Asp Pro His Arg Ala Pro Lys Asn Thr G u Leu Ser Thr Arg 435 440 445 Tyr Ala Lys Leu Arg Al a Ala Thr G n y Trp Tyr 450 455 460 <210> 387 <211> 1139 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (1)..(1116) <223> FRXA01927 <400> 387 aat Asn 1 ggc Gy tct Ser aat Asn tgg Tr p tct Ser ct a Leu gcc Al a gca Al a cct Pr o 145 acc Thr act Thr gat Asp act Thr gcg Al a ttg Leu acg Thr gcc Al a aat Asn 115 acg Thr gat Asp caa G n tct Ser 5 gct Al a acc Thr acg Thr gga Gy t ac Tyr gcg Al a at t SIle gat Asp agc Ser agc Ser 165 gcc Al a gcg Al a aaa Lys gcg Al a cgc Ar g 70 tac Tyr ct g Leu gcg Al a aat Asn gt g Val 150 gt c Val cag G n gt a Val at g bet tcg Ser gga G y agc Ser ggc Sy gga Sy gct Al a 135 tcg Ser cac Hi s gct Al a gat Asp cgg Ar g 40 gt g Val cgc Ar g acg Thr cat Hi s aca Thr 120 gcg Al a at c SIle gat Asp gcg Al a gcc Al a tgg Tr p at g Net aaa Lys cgt Ar g gag G u 105 act Thr gct Al a ggc Sy cca Pr o ttg Leu 10 acg Thr at g tet ttg Leu gt c Val gat Asp 90 gt g Val cca Pr o gcg Al a acc Thr tct Ser 170 gat Asp gga G y cgt Ar g cct Pr o acc Thr 75 cgt Ar g gaa G u ggt Sy ctt Leu tct Ser 155 ggt Sy ct c Leu agt Ser gat Asp cat Hi s gac Asp gcg Al a ctt Leu gga Sy ggc Sy 140 ggc Sy ttg Leu aat Asn gt g Val cat Hi s gat Asp cat Hi s tgg Tr p cct Pr o gt g Val 125 ctt Leu gtt Val gt c Val gag G u t at Tyr gaa G u ttc Phe ggt G y cgc Ar g gaa G u 110 aaa Lys gat Asp gcc Al a act Thr gag G u gtt Val cca Pr o ct c Leu gat Asp acc Thr ct c Leu gtt Val ttg Leu ggc Sy ggt Sy 175 at c SIe gct Al a gaa G u acc Thr gct Al a gat Asp ctg Leu gct Al a cag G n at g et 160 ttc Phe gcc gat gcc acg ggt gcg tat ttc ccg Ala Asp Ala Thr Oy Ala Tyr Phe Pro ctg gcc tgc acg ctt aat ggc Leu Ala Cys Thr Leu Asn y Page 501 Gene Sequence 24-2-06.txt 180 185 190 gca ccg gtg ttg gaa ttc ggc cgc cgc att ctg ggc gtg gaa tgg gaa 624 Ala Pro Val Leu G u Phe G y Arg Arg lie Leu G y Val G u Trp G u 195 200 205 gag ttc gat gcg ctt gca ctg gct gct caa ccc ggt tca ggt ggc gtg 672 G u Phe Asp Ala Leu Al a Leu Ala Ala G n Pro y Ser Gy y Val 210 215 220 acg ctc cag cct tat ttg gag ggc gag cgt acg ccg aat cgt ccc gca 720 Thr Leu G n Pro Tyr Leu G u y G u Arg Thr Pro Asn Arg Pro Ala 225 230 235 240 gca cgt ggc gtt ttg gct gga cta aac tgt gca acg acc cgc gag gac 768 Ala Arg y Val Leu Al a y Leu Asn Cys Ala Thr Thr Arg G u Asp 245 250 255 ttt gcc cga gca act gtt gaa ggc ttg ttg ttg gca ttg gat gat gct 816 Phe Ala Arg Ala Thr Val G u y Leu Leu Leu Ala Leu Asp Asp Ala 260 265 270 gta acg gcg ctg gtt gag gcc acg gga gtg ccc gtt cag cgc atc cag 864 Val Thr Ala Leu Val G u Ala Thr y Val Pro Val G n Arg Ile G n 275 280 285 ctc atc ggt ggc ggc gcg cgt tca cag gcg gtt cgt gag att gcc cct 912 Leu lle y G y G y Ala Arg Ser n Ala Val Arg u Ile Ala Pro 290 295 300 gag att ttc ggc cat gag att gtg gtt cca gaa ccc gct gaa tat gtg 960 G u IIe Phe G y His G u IIe Val Val Pro G u Pro Ala G u Tyr Val 305 310 315 320 gcg ttg ggt gca gct cgt cag gcg gca tgg gcg ctg tcg ggt gag gcc 1008 Ala Leu y Ala Ala Arg G n Ala Ala Trp Ala Leu Ser y G u Ala 325 330 335 acg cca ccg cag tgg cca act ccc ggt tcc gat ccg cac cgc gca cct 1056 Thr Pro Pro G n Trp Pro Thr Pro G y Ser Asp Pro His Arg Ala Pro 340 345 350 aaa aac act gag ctg agc acg cgt tat gcg aag ctg cgt gct gca acg 1104 Lys Asn Thr G u Leu Ser Thr Arg Tyr Ala Lys Leu Arg Ala Ala Thr 355 360 365 cag ggt tgg tac tagagctcga tattgtcgat caa 1139 G n y Trp Tyr 370 <210> 388 <211> 372 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 388 Asn Asp Thr Arg Ser Al a n Ala Ala Leu Asp Leu Asn G u G u Ile 1 5 10 Sy y Asp G n Ala Al a Val Asp Ala Thr y Ser Val Tyr Val Ala 25 Ser Leu Thr Ala Thr Lys IVet Arg Trp IVet Arg Asp His G u Pro G u 40 Asn Ala Ala Arg Thr Ala Ser Val Iet Leu Pro His Asp Phe Leu Thr 55 Page 502 Gene Sequence 24-2-06.txt Tr p Ser Leu Al a Al a Pr o 145 Thr Al a Al a G u Thr 225 Al a Phe Val Leu G u 305 Al a Thr Lys G n Leu Thr Al a Asn 115 Thr Asp G n Al a Val 195 Asp G n Gy Ar g Al a 275 Gy Phe Gy Pr o Thr 355 Tr p Gy Tyr Al a SIle Asp Ser Ser 165 Gy G u Leu Tyr Leu 245 Thr Val Gy Hi s Al a 325 Tr p Ar g Ty r Leu Al a Asn Val 150 Val Al a Phe Al a Leu 230 Al a Val G u Al a G u 310 Ar g Pr o Sy Arg Lys Val Thr Asp His G y Asp Ala Ser Gy Gy Al a 135 Ser Hi s Tyr Gy Leu 215 G u Gy G u Al a Ar g 295 I le G n Thr Thr Hi s Thr 120 Al a SIle Asp Phe Ar g 200 Al a Gy Leu Gy Thr 280 Ser Val Al a Pr o Ar g 360 Ar g G u 105 Thr Al a Gy Pr o Pr o 185 Ar g Al a G u Asn Leu 265 Gy G n Val Al a Gy 345 Asp 90 Val Pr o Al a Thr Ser 170 Leu SIle G n Ar g Cys 250 Leu Val Al a Pr o Tr p 330 Ser Ar g G u Sy Leu Ser 155 Sy Al a Leu Pr o Thr 235 Al a Leu Pr o Val G u 315 Al a Asp Al a Leu Sy Sy 140 Sy Leu Cys Gy Sy 220 Pr o Thr Al a Val Ar g 300 Pr o Leu Pr o Tr p Pr o Val 125 Leu Val Val Thr Val 205 Ser Asn Thr Leu G n 285 G u Al a Ser Hi s Ar g 365 Thr Leu Val Leu Sy Sy 175 Asn Tr p Sy Pr o G u 255 Asp SIe Al a Tyr G u 335 Al a Leu Ser Thr Tyr Ala Lys Leu Al a Al a Thr <210> 389 <211> 844 <212> DNA <213> Corynebacter i um gl utam cum <220> Page 503 Gene Sequence 24-2-06.txt <221> CDS <222> (101)..(844) <223> RXA02729 <400> 389 gaattaattg caggcacggt acctagcgtg acgaaaacta cgatccccac tgcccttatt catcgtgaat caatcatcaa ctccacttta aggaagaagg agt Ser gat Asp ct g Leu gct Al a gt c Val t ca Ser ctt Leu at c SIle t cc Ser cct Pr o 150 gt g Val cgt Ar g gac Asp act Thr t ca Ser acc Thr agc Ser gcg Al a acc Thr gca Al a at c SIle ggc G y 120 ct c Leu gat Asp aat Asn gat Asp ctt Leu 200 ttg Leu ggt Gy aaa Lys ggc Gy gcg Al a caa G n at g bet act Thr aac Asn gag G u ttc Phe gct Al a 170 ttg Leu act Thr gag G u acc Thr gtt Val aca Thr caa G n at g bet 75 t cc Ser gt g Val gct Al a aac Asn gag G u 155 cca Pr o ct g Leu cca Pr o cag G n gac Asp caa G n gt g Val tta Leu gct Al a gcg Al a tct Ser tct Ser gcc Al a 140 cgt Ar g gtt Val gt c Val gcg Al a ggt G0y 220 gt c Val cac Hi s gct Al a gcc Al a gag G u gcc Al a gat Asp 110 acc Thr at t l Ie gtt Val ccc Pr o gaa G u 190 acg Thr act Thr gtt Val 15 cct Pr o ggt Gy aaa Lys gcg Al a act Thr 95 ggg G y gcg Al a gt g Val gat Asp gt g Val 175 cac Hi s tct Ser t cc Ser gt c Val gaa G u ggc Gy gt c Val ct g Leu 80 gt g Val gaa G u gaa G u ttg Leu ctt Leu 160 gga Gy gaa G u gat Asp gt g Val atg gac IVet Asp 1 gga t cc G y Ser cct gga Pro G y aaa ggc Lys G y acc at g Thr Nbet aca cat Thr His gac ggt Asp y aac acc Asn Thr ttt gtt Phe Val 130 ctt cag Leu G n 145 tca caa Ser G n cat gat His Asp ggc gct G y Al a ccc caa Pro G n 210 gt g at g Val Vbet 225 t cc cca at g Ser Pro bet at c aat gcc lI e Asn Al a gaa acc ct c G u Thr Leu gcc aac caa Al a Asn G n atc ggt gcg i e G y Al a ttg cgt caa Leu Arg G n ccc act ggt Pro Thr G y 100 atc atc gtt Ile Ile Val 115 gat aaa cac Asp Lys Hi s ggt gag at c G y G u lie gga cgt gtg G y Arg Val 165 cag ctg cgt G n Leu Arg 180 ctg gtg ctg Leu Val Leu 195 agt ttg gtc Ser Leu Val aca ct t ggt Thr Leu G y gcc gaa ggt gct ctg gtt ggc acg ccg ggc caa ctc acg gca att cct Page 504 Gene Sequence 24-2-06.txt Ala Gu Gy Ala Leu Val Oy Thr Pro Gy n Leu Thr Ala lie Pro 230 235 240 245 acc cca aag 844 Thr Pro Lys <210> 390 <211> 248 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 390 Met Asp Ser Pro Met Ser Asn Ser Thr y Thr Asp Ile Val Val Val 1 5 10 G y Ser I I e Asn Ala Asp Leu Thr Ala Lys Val G n Arg His Pro G u 25 Pro y u Thr Leu Leu y Ser y y Thr Val Ser Ala y y 40 Lys y Ala Asn n Ala Val Ala Ala Ala n Leu y Ala Lys Val 55 Thr [et Ile y Ala Val y Thr Asp G n et Al a y u Ala Leu 70 75 Thr Hi s Leu Arg G n Ser G y Al a Asp IVet Ser Al a I e Al a Thr Val 90 Asp y Pro Thr y Leu Ala lle Ile Thr Val Ser Asp Asp Gy u 100 105 110 Asn Thr Ile Ile Val Ile Pro G y Ala Asn Ala Ser Val Thr Al a u 115 120 125 Phe Val Asp Lys His Ser G n Leu IIe G u Asn Ala G y IIe Val Leu 130 135 140 Leu On y u lie Pro Ala Asp y Phe u Arg Ala Val Asp Leu 145 150 155 160 Ser G n y Arg Val Val lie Asn Leu Ala Pro Val Val Pro Val y 165 170 175 His Asp G n Leu Arg Arg Ala Asp Pro Leu Leu Val Asn G u His G u 180 185 190 G y Al a Leu Val Leu Asp IVet Leu G y Thr Pro Al a Thr Thr Ser Asp 195 200 205 Pro G n Ser Leu Val Thr G u Leu Leu G u G n y Phe Thr Ser Val 210 215 220 Val et Thr Leu G y Al a u y Ala Leu Val y Thr Pro G y n 225 230 235 240 Leu Thr Ala lie Pro Thr Pro Lys 245 <210> 391 <211> 1026 <212> DNA <213> Cor ynebact er i um gl ut am cum Page 505 Gene Sequence 24-2-06.txt <220> <221> CDS <222> (101)..(1003) <223> RXA02797 <400> 391 acagtctcat gaagccataa taaccacctt ctacaaagat ccttatgaaa acgtttgcat aactccgcta aggatgttcc cgacgtagaa tggaataacc gt c Val cac Hi s gca Al a gca Al a cca Pr o t cc Ser gac Asp aat Asn at c SIle at t SIle 150 gag G u gag G u t cc Ser gcc Al a gt c Val gca Al a ggc Gy gt c Val tta Leu gt a Val gag G u gat Asp 120 ttg Leu cac Hi s gag G u gag G u gat Asp 200 gtt Val t cc Ser ggc Gy ggc Gy ttt Phe ttc Phe gac Asp aat Asn gt a Val caa G n at g Net t cc Ser 170 ggc Gy cac Hi s tta Leu at c SIle gaa G u gcc Al a gt c Val ctt Leu 75 acc Thr at c SIle agc Ser ggt Gy ggt Gy 155 gcg Al a ct g Leu gag G u acg Thr aac Asn aca Thr aac Asn ggc Gy cgt Ar g acc Thr gtt Val agc Ser gag G u 140 cgc Ar g tta Leu at t SIle ct c Leu ctt Leu 220 gct Al a ct g Leu cag G n 45 gct Al a tcg Ser ggg Gy gt c Val caa G n 125 at c SIle gt c Val ctt Leu ct g Leu gcg Al a 205 gga Gy gat Asp ttg Leu gca Al a gt g Val t ca Ser ctt Leu at c SIle 110 t cc Ser cct Pr o gt g Val gag G u gat Asp 190 caa G n t cc Ser ctt Leu 15 ggc G y gt a Val ggc Gy ggc Sy gca Al a 95 ccc Pr o gct Al a gcg Al a gt g Val gct Al a 175 caa G n gct Al a gca Al a aat Asn agt Ser gct Al a aag Lys gt c Val 80 gtt Val ggc Sy ctt Leu gat Asp aat Asn 160 gat Asp ttc Phe ct c Leu ggc G y at g let 1 gtt Val ggt G y gcc Al a gat Asp gac Asp at c SIle gcg Al a tta Leu ggc Sy 145 ct a Leu ccg Pr o ggg G y ct c Leu gcg Al a 225 ct c Leu gga G y gct Al a cct Pr o ctt Leu acc Thr aat Asn gct Al a 130 ttc Phe gcg Al a at c SIle gca Al a gac Asp 210 ttg Leu gtt Val cac Hi s ctt Leu t ac Tyr acg Thr gtt Val t cc Ser 115 gaa G u aaa Lys ccc Pr o at c SIle ggc G y 195 gcc Al a gt c Val gac Asp at c SIle caa G n gct Al a gca Al a gcc Al a 100 ct g Leu gct Al a gag G u gt c Val gcc Al a 180 at c SIle ggt G y gcc Al a aat aat cga Asn Asn Arg att I e cgc Ar g act Thr ggt G y gcc Al a gta Val aaa Lys gtc Val gga Sy gcc Al a at c SIe 165 aat Asn gat Asp ttc Phe gat Asp Page 506 gcc acc ggt at c acg gac at c gcc a Al a Thr 03 y I I e Thr Asp I I e Al a T 230 235 acc acg gga gcc ggt gac gct ttt g Thr Thr 03 y Al a 03 y Asp Al a Phe A 250 at t aaa ggc gat t cg ct t at c gac g Ilie Lys G31y Asp Ser Leu Ilie Asp P 265 2 ggc gct tac tcg gtg caa acc gcc g G31y Al a Tyr Ser Val O31n Thr Al a C 280 285 gcg agc gt t t ca ct t ccc t ct gt t t Al a Ser Val Ser Leu Pr o Ser Val 295 300 <210> 392 <211> 301 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn Sequence 24-2- 06. txt ca cca acg gtg cag gca gtt gac h r Pr o Thr Val O31n Al a Val Asp 240 245 cc gga gcc ttc tgc gca cga cta I a G31y Al a Phe Cys Al a Ar g Leu 255 260 cc gcc acc cac gca gca cgc gt c I a Al a Thr Hi s Al a Al a Ar g Val 70 275 ga gcg caa gcg t cc t at ccg gac ~y Al a O31n Al a Ser Tyr Pr o Asp 290 aaaaaaact at t taagaag agg 835 883 931 979 1 026 <400> 392 I'b t As n As n Ar g I Ie Val ValI G31y Al a As p As p I Ie Al a Le u G31y 145 Le u Pr o G31y Le u ValI Hi s Le u Tyr Thr ValI Ser 115 (31u Lys5 Pr o I Ie G31y 1 95 Al a Ar g Thr G31y Al a ValI Lys5 ValI G31y Al a I Ie 1 65 As n As p Ph e Hi s Al a Al a Pr a 70 Ser As p As n I Ie I Ie 150 (31u (31u Ser Al a ValI Pr a G31y As p Al a (31u G31y Cys Le u 135 Hi s ValI Hi s vb t Ser 215 ValI Al a G31y 40 ValI Le u ValI (31u As p 120 Le u Hi s (31u (31u As p 200 ValI G31y Pr a Lys5 Al a (31u As p As n 1 05 Tyr Le u Thr Lys5 Al a 1 85 Pr a ValI I Ie (31u Al a ValI Le u Thr I Ie Ser G31y G31y 1 Al a Le u (31u Al a Le u O31n Al a Ser O31y ValI O31n 125 li1e ValI Le u Le u Al a 205 As p Le u Al a ValI Ser Le u I Ie 110 Ser Pr a ValI O31u As p 1 O31n Le u O31y ValI O31y O31y Al a Pr a Al a Al a ValI Al a 1 O31n Al a Leu Thr Leu (3y Ser Al a (3y 220 Page 507 Gene Sequence 24 Ala Leu Val Ala Asp Ala Thr Oy Ile Thr Asp 225 230 235 Val G n Ala Val Asp Thr Thr y Ala y Asp 245 250 Phe Cys Ala Arg Leu Ile Lys y Asp Ser Leu 260 265 His Ala Ala Arg Val G y Ala Tyr Ser Val G n 275 280 Ala Ser Tyr Pro Asp Ala Ser Val Ser Leu Pro 290 295 <210> 393 <211> 1161 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1138) <223> RXA02730 <400> 393 ccaacatcgc cttgcacgta ataggttaaa acacaagtga cgattacata aaggtagata atgagataaa gcgaggcgct -2-06. txt Ile Ala Thr Pro Thr 240 Al a Phe Al a y Al a 255 I le Asp Al a Al a Thr 270 Thr Al a y Al a n 285 Ser Val 300 atgtaatcgt ttgcagcaat ttc Phe gcc Al a act Thr aat Asn gt g Val gaa G u aac Asn acc Thr gcg Al a ccg Pr o gca Al a gaa G u caa G n gtt Val caa G n aat Asn cat Hi s 120 caa G n ctt Leu cga Ar g at c SIle cgt Ar g agt Ser acc Thr gat Asp gt c Val gag G u aaa Lys gca Al a caa G n gcg Al a tt g Leu 75 gcc Al a gcg Al a gat Asp gac Asp gat Asp ct a Leu caa G n ctt Leu at t SIle agc Ser acc Thr gga Sy ttg Leu gt c Val gcg Al a tta Leu 45 cgc Ar g aac Asn aaa Lys act Thr at c SIle 125 cag G n gct Al a gat Asp gcc Al a agt Ser cat Hi s gct Al a at g Met 110 at c SIle aag Lys caa G n ccg Pr o gat Asp cgc Ar g ttc Phe 80 ctt Leu ggg Sy gt a Val at g Net 1 gca Al a gcg Al a ct g Leu agc Ser gcc Al a gcc Al a tct Ser cct Pr o ggt G y gtt Val ggt G y aac Asn gca Al a acg Thr ttg Leu aat Asn 130 gcg acg gaa Al a Thr G u t cc Ser gca Al a cgg Ar g at t SIle gtt Val at c SIle 100 ttt Phe gaa G u aaa Lys at c SIe tcg Ser gcc Al a ggt Sy act Thr acc Thr ct c Leu tgc Cys caa gga atg cca gtg gtg ttg G n y Net Pro Val Val Leu Page 508 Gene Sequence 24-2-06.txt gtt Val 150 aac Asn aac Asn ggt Gy at t SIle ttt Phe 230 gcc Al a gct Al a cat Hi s aat Asn at t SIle 310 ctt Leu gga Gy cga Ar g caa G n ttg Leu gag G u 200 gaa G u ggc Gy gat Asp ttg Leu ctt Leu 280 gaa G u ggc Gy cat Hi s ccc Pr o gag ctt G u Leu cca gga Pro G y 170 ccg att Pro Ile 185 cga tta Arg Leu cag ct c G n Leu gct acg Al a Thr tct atg Ser Mbet 250 gtt atc Val lile 265 ttt gcc Phe Al a caa ct a G n Leu acg gta Thr Val cgt gaa Arg G u 330 aat gag Asn u 345 cca Pr o 155 at c SIle ggt Gy gag G u gtt Val aaa Lys 235 at g bet ggc Gy ct g Leu gcc Al a cct Pr o 315 t ca gga Gy gcc Al a t ac Tyr gat Asp ttt Phe 220 ttg Leu acg Thr aag Lys caa G n caa G n 300 agc Ser at c gac Asp gca Al a ct c Leu ttc Phe 205 ct g Leu ct c Leu at c SIle gat Asp cct Pr o 285 cga Ar g gt g Val at c t cc Ser gca Al a t ca Ser 190 aaa Lys ggt Gy gat Asp ggt Gy gt c Val 270 cat Hi s gca Al a acg Thr aac acc Thr gt a Val 175 ggt Gy gca Al a ggg Gy caa G n gt c Val 255 agc Ser ccg Pr o gt g Val aaa Lys t cc Ser 335 145 at c cca acg I e Pro Thr 160 gaa ct c ct g G u Leu Leu ccc atg gac Pro IVet Asp gcc tgc gcc Al a Cys Al a 210 tac gaa caa Tyr G u G n 225 gga gct aaa G y Al a Lys 240 att gaa gcc Ile G u Al a gtg att ggt Val Ile G y ttg aca gtg Leu Thr Val 290 tct atc ctc Ser Ile Leu 305 act acg at c Thr Thr Ile 320 act tta agg Thr Leu Arg gcg Al a gct Al a acc Thr 195 aac Asn agc Ser act Thr tgc Cys ttt Phe 275 at t SIle acc Thr ccc Pr o aag Lys acc Thr cac Hi s 180 t ca Ser t cc Ser gtt Val ctt Leu cat Hi s 260 gat Asp gat Asp gaa G u act Thr aag Lys 340 tct Ser 165 aac Asn aca Thr aaa Lys gga Sy ttt Phe 245 aag Lys aca Thr caa G n tta Leu gcc Al a 325 gat Asp 595 643 691 739 787 835 883 931 979 1027 1075 1123 1161 Ser Ile lie Asn taactcaacc ggtaccgaca ttg <210> 394 <211> 346 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 394 IVet Ala Thr G u Lys Phe Arg Pro Thr Leu Lys Asp Val Ala Arg G n 1 5 10 Ala y Val Ser lie Al a Thr Ala Ser Arg Ala Leu Ala Asp Asn Pro 25 Ala Val Ala Ala Ser Thr Arg G u Arg lie n n Leu Ala Ser Asp Page 509 Gene Sequence 24-2-06.txt Arg Ser Ser Arg Leu Ser Al a Al a Ser Pr o Met 145 Pr o Leu let Cys G u 225 Al a Su Ile Thr I le 305 Thr Leu Tyr Thr Met l Ie G u 115 G u Val Al a Al a Thr 195 Asn Ser Thr Cys Phe 275 I le Thr Pr o Lys Al a Gy Thr Thr Leu Cys Leu Ser 165 Asn Thr Lys Gy Phe 245 Lys Thr G n Leu Al a 325 Asp Asn Val 70 G u Asn Thr Al a Val 150 Asn Asn Gy I e Phe 230 Al a Al a Hi s Asn SIle 310 Leu Gy Al a I e I e Ser Ser Asn 135 Asp Pr o Al a Ar g Gy 215 G u Gy Gy Pr o Val 295 Al a SIle Leu Al a Ar g Al a Pr o Ser G u 105 Gy Leu G u Pr o Pr o 185 Ar g G n Al a Ser Val 265 Phe G n Thr Ar g Asn 345 Leu Al a Al a Asp Asp Pr o 155 I le Gy G u Val Lys 235 let Gy Leu Al a Pr o 315 Ser Asn Lys Thr SIle 125 G n Asp Al a Leu Phe 205 Leu Leu SI e Asp Pr o 285 Ar g Val SIle Hi s Al a vet 110 SIle Lys Ser Al a Ser 190 Lys Gy Asp Gy Val 270 Hi s Al a Thr Asn Tyr Gy Ser Cys G n Thr Val 175 Gy Al a Gy G n Val 255 Ser Pr o Val Lys Ser 335 <210> 395 <211> 483 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(460) Page 510 ene Sequence 24- 2- 06. txt <223> RXA02551 <400> 395 gctgcacaaa ttccgcgaaa gggtatcacc atcgacatca ttaaaaaaac accgtcgatt tcatttcctt ctcctattac cagat gaaga ccgt gagat c at g t cc gt a t gt gaa 11! [Vbt Ser Val Cys (31u gcc A a gga G31y g at As p c ag O31n ct t Le u aaa Ly s aag Ly s ct g Le u aac As n gt c ValI ct c Le u cca Pr o g ac As p ct c Le u tt t Ph e t cc Ser 120 g aa G31u CCC Pr o t tg Le u tt c Ph e ccc Pr o g ac As p t gg Tr p t ac Tyr ct c Le u at c I Ie gt c ValI ggc G31y ggC G31y cgc Ar g t ca Ser gcg A a gt c ValI 45 g aa G31u cca Pr a t ca Ser g ac As p ac c Th r gct A a ct g Le u aac As n ac a Th r gga G31y at c I Ie 110 ggt G31y 15 t cc Ser aac As n gga G31y gt a ValI at c I Ie 95 at c I Ie ggc G31y g aa G31u aac As n ct a Le u aac As n tt g Le u gcc A a aac As n ggt G31y t gg Tr p gca A a g ac As p cgc Ar g aat As n at c lIe c aa O31n cgc Ar g g ac As p cgc Ar g aag Ly s 1 00 g aa (31u t aagaaat aa aggt aggt gt cac <210O> 396 <211> 120 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 396 I'bt Ser Val Cys Ou Ala His Asn Pro 1 5 Gy Asn Ilie lie Gy G31y ValI Val Ser 25 Trp G31y Tr p O31n Val Asp Pr o Leu (31y 40 Ty r Tr p O31u Ar g Tr p O31n Lys Pr o Leu G31y Ala Lys As p Val Leu I Ie As p (31y 70 As p As p Ty r Ar g I Ie Lys Ty r Leu As p Lys Ar g Ty r Lys Lys Lys Ser Phe As p 100 105 31u As n 10 Pr o Thr I Leu ArgI Phe I I e Pr o Ser As p (1y 90 Tr p Cys Page 511 Ser A a ValI O31u Pr o Ser As p Th r A a Le u As n Th r G31y I Ie 110 G31y Se r As n G31y ValI I Ie lie Gene Sequence 24-2-06.txt Thr Asn Gy u Ser Leu u Ser 115 120 <210> 397 <211> 795 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(772) <223> RXA01325 <400> 397 gcgcagcgct cggacgatta cgaacttcag gagaactcgg cctggaaatt t tcccacact aagtcaggtc taagtagggt ggtcattcgt tgcattctac t cc Ser gaa G u ct g Leu gca Al a ccg Pr o gcc Al a gt a Val at c l Ie aca Thr at t l Ie 150 gcg Al a cgg Ar g t cc Ser acc Thr gt c Val gcc Al a ggc Gy cag G n aaa Lys gag G u 120 gtt Val gct Al a ggg Gy agg Ar g at g let t cc Ser acg Thr gaa Gu acc Thr gga Gy gga Gy gtt Val agt Ser gct Al a act Thr 170 gga Gy gcc Al a gaa Gu at c SIle gct Al a ccc Pr o 75 gct Al a gat Asp cca Pr o gat Asp gac Asp 155 gtt Val at t SIle caa G n ct g Leu tgt Cys gga Gy gcg Al a t cc Ser gcc Al a tct Ser gaa G u 140 ttc Phe gag G u aag Lys at c SIle gcc Al a gt c Val 45 at t SIle ttg Leu gaa G u aat Asn cct Pr o 125 gca Al a gct Al a gct Al a gct Al a ctt Leu gca Al a ccc Pr o cga Ar g gt g Val gt a Val agg Ar g 110 gt g Val at t l Ie aaa Lys gtt Val gcc Al a t ac Tyr at a l Ie agc Ser gcc Al a gcc Al a 80 gtt Val ct g Leu ct g Leu act Thr t cc Ser 160 gt g Val at g Met 1 acc Thr gat Asp at g Met acc Thr gcc Al a gtt Val cag G n aaa Lys gca Al a 145 acg Thr at g let gat atg acg Asp IVet Thr ct c Leu t cc Ser gt c Val gt c Val gaa G u ttg Leu gaa G u ttc Phe 130 gt g Val gga Gy gct Al a ct c Leu gca Al a aac Asn gca Al a gcg Al a gat Asp at t SIle 115 at c SIle aat Asn ttc Phe tcg Ser gga Gy at t l Ie ct a Leu gga Gy cgc Ar g at t SIle 100 gt g Val ct c Leu gcg Al a cac Hi s gct Al a 180 att I e cca Pr o gag G u act Thr ttc Phe ctt Leu gcg Al a gca Al a gaa G u ttg Leu cca Pr o 165 tct Ser ggt ggg gtg aaa act tgg gaa Gy Gy Val Lys Thr Trp u Page 512 Gene Sequence 24-2-06.txt 185 190 195 gat gcg gtg gcg ttt gtt gaa gca ggg gct act cgc att gga act tct 739 Asp Ala Val Ala Phe Val G u Ala Gy Ala Thr Arg lie y Thr Ser 200 205 210 aat gcg gga gcc att ttg gag ggt gcg ccg gag tagtttggcg ttctaatcgg 792 Asn Ala G y Ala lie Leu G u G y Ala Pro G u 215 220 gac 795 <210> 398 <211> 224 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 398 IVet Asp IVet 1 Thr Leu Leu Asp Ser Al a [et Val Asn Thr Val Al a Ala G u Ala Val Leu Asp Gn G u lie 115 Lys Phe Il e 130 Al a Val Asn 145 Thr G y Phe et Al a Ser Val Lys Thr 195 Arg lie G y 210 Thr I e Ser 5 G y Pro G u l e G u Leu Leu Thr Al a G y Phe Pr o 70 Arg Leu Ala I le Al a Val 100 Val Ala lle Leu G u Thr Al a Leu II e 150 Hi s Pr o Al a 165 Al a Ser Ar g 180 Trp u Asp Thr Ser Asn Ar g Val Gy Lys 55 Hi s Val Val Ar g Al a 135 Al a Sy Sy Al a Al a 215 Thr Asn 25 Gy G n Lys Ser G u 105 Al a Val Sy Al a Val 185 Al a Al a Al a Su I e Al a Pr o Al a Asp Pr o Asp Asp 155 Val SIle Val Leu G n Leu Cys Sy Al a Ser Al a Ser G u 140 Phe G u Lys G u G u 220 SIle Al a Val I le Leu G u Asn Pr o 125 Al a Al a Al a Al a Al a 205 Sy Leu Al a Pr o Ar g Val Val Ar g 110 Val l Ie Lys Val Al a 190 a y Al a Asp Phe Asn Val Lys Asp Leu Val Val Thr Ar g 175 Sy Al a Pr o <210> 399 <211> 684 <212> DNA Page 513 ene Sequence 24- 2- 06. txt <213> Cor ynebact er i urn gi ut ani curn <220> <221> OIDS <222> (661) <223> RXA00195 <400> 399 tcgcagt cat cat gcaggca t aacct gaaa cccat ccgt t t ggat t gccc caaat gggt g t agt gggt gc gt t t acccaa caagt gcaag aat gggagt c gt g act aaa aag at c Val Thr Lys Lys I I e ct t Le u at t I Ie ggt G31y gct A a t cg Ser at t I Ie ggt G31y cat Hi s gt t ValI c ac Hi s 1 50 gcg A a ggg G31y tt g Le u g at As p c ag O31n g ac As p gct A a gt g ValI tt g Le u gcc A a 120 t ca Ser gcc A a at t I Ie ag a Ar g ag c Ser t ca Ser g ac As p gt t ValI G31y ac c Thr ct t Le u gct A a gca A a gcg A a ct g Le u 1 70 act Thr gac As p ct c Le u gcg A a ggc G31y 75 aca Thr gcg A a aac As n aag Ly s cag O31n 155 aca Thr ggt O31y aag Ly s gt t ValI gt t ValI gag O31u aag Ly s gca A a aaa Ly s ct g Le u 140 t gt cys gct A a t cg Ser tt t Ph e at t I Ie 45 gct A a at c I Ie gcc A a ac g Thr g aa O31u 125 ggg O31y tt g Le u t cg Ser at t I Ie gag O31u t cg Ser g at As p at c I Ie g ac As p ct g Le u 110 t cg Ser c ag O31n cgt Ar g ggc G31y gga G31y 15 gt g ValI c ag O31n gca A a t ct Ser act Thr 95 gcc A a ct g Le u at c I Ie t cg Ser gga O31y 1 75 act Thr gt g ValI gcg A a c ag O31n gga O31y gt g ValI act Thr gt t ValI at t I Ie ggt O31y 1 cct Pr o gcg A a at c I Ie c ag O31n gcc A a g at As p aat As n g aa O31u ggt O31y 130 gt c ValI cgt Ar g ag g Ar g ct g Le u gct A a tt g Le u gca A a gct A a gct A a t ct Ser 115 ggt O31y g at As p g at As p gct A a g ac As p gcg A a ggg O31y gt a ValI gcg A a ct g Le u 1 00 ggt O31y gag O31u t cg Ser gag O31u gga O31y 1 tgt ggg agg Cy s O31y Ar g 1 t gact cccga gcaggcagca gcg <210O> 400 <211> 187 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn Page 514 Gene Sequence 24-2-06.txt <400> 400 Val Thr Lys Lys Ile Leu Ile Leu y Ser Thr Gy Ser lie 1 G n Gy G n Al a Thr Leu Leu Al a Pr o 145 Thr Phe Leu Al a Leu Al a Al a Al a Ser 115 Gy Asp Asp Al a 5 Val Gy Leu l Ie Lys Val Al a Phe G u Val 165 Al a Ser Al a Lys Leu Ser Leu Thr 135 Ser Ar g Asp G n 40 Asp Al a Val Leu Al a 120 Ser Al a SIle Asn Pr o Lys Leu G u Gy 105 Leu Lys Vet Val Ser Asp Val Gy Thr 90 Leu Al a Al a Al a Leu 170 Asp Leu Al a Gy 75 Thr Al a Asn Lys G n 155 Thr Lys Val Val G u Lys Al a Lys Leu 140 Cys Al a Phe SIle Al a SIle Al a Thr G u 125 Gy Leu Ser G y Thr Val Val G n Al a Al a n Ser G y Thr Val Al a Thr Leu Val Ile Ile Ser G y 160 G y Pro 175 Pro G y Arg Arg Cys y Arg 185 <210> 401 <211> 738 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(715) <223> RXA00196 <400> 401 ctgccatggc gcagtgtttg cgttcgggta ctcgtgatga cagcttcggg cggacctttc agggctggac cagggagaag ccc Pr o acg Thr gcg Al a cat cag G n aac Asn ct g Leu cag gca Al a gcc Al a ttt Phe at c gcg Al a acc Thr gaa t cu at c cca Pr o at t SIle gat Asp 45 t cc acg Thr aat Asn gcg Al a at g tgg gcg Trp Al a 15 aaa ggc Lys G y gat ct c Asp Leu atc acg Page 51 ggttgcgcgg attgtgctga atg tgg gag gtg act Net Trp G u Val Thr 1 atg ggg cag atg aac IVet y G n Iet Asn ctc gaa ctc atc gag Leu G u Leu Ile G u att gat gtg acg gtg Ile Asp Val Thr Val ttt acg gat ggt gcg His Pro On Ser Ile Ile His 60 Gene Sequence 24-2-06.txt Ser Met lie Thr Phe Thr Asp y Ala acc atc gcg cag gcg tcg cca cca tcg Thr lie Ala O n Ala Ser Pro Pro Ser 75 gcg ctt gat tgg cca cat cgg gtg ccg Ala Leu Asp Trp Pro His Arg Val Pro ttc acc gct gct cat acc tgg gct ttt Phe Thr Ala Ala His Thr Trp Ala Phe 105 110 ttc cct gcg gtg cag ctg gct agg cac Phe Pro Ala Val G n Leu Ala Arg His 120 125 tac ccc gcg gtg tat aac gcc gcc aac Tyr Pro Ala Val Tyr Asn Ala Ala Asn 135 140 ttg cgc ggg cga atc aag ttt ccg cag Leu Arg y Arg lie Lys Phe Pro G n 150 155 gtc ctc caa gga gct tct cag ttt gct Val Leu G n Gy Ala Ser G n Phe Ala 170 gat att ttg gca acc gaa tct gag gca Asp Ile Leu Ala Thr u Ser G u Al a 185 190 atc aac cgg ttg gca acc aac ttg taa! Ile Asn Arg Leu Ala Thr Asn Leu 200 205 <210> 402 <211> 205 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 402 et Trp u Val Thr Pro u n Ala 1 5 IVet G y G n Iet Asn Thr Leu Asn Ser 25 Leu G u Leu Ile G u Al a Thr Leu Leu 40 Ile Asp Val Thr Val His Pro G n Ser Phe Thr Asp y Ala Thr Ile Ala n 70 Leu Pro lie Ala Leu Ala Leu Asp Trp G n Pro Ala Leu Asp Phe Thr Ala Ala 100 105 Val Asp Asp Ala Ala Phe Pro Ala Val aaa Lys gct Al a ccg Pr o gca Al a gag G u gt g Val 160 gt a Val gcg Al a ct g Leu cag G n gt g Val aag Lys gcg Al a 145 gac Asp gca Al a cgt Ar g ccg Pr o ccg Pr o gat Asp caa G n 130 gct Al a gt g Val t ca Ser gcg Al a at c SIle gcg Al a gat Asp 115 aaa Lys gag G u gt g Val cac Hi s aat Asn gcg Al a ctg Leu 100 gcc Al a ggc G y gcg Al a gac Asp gtc Val 180 gct Al a gctaagg agcttccgcc tcg Al a Al a I 10 Al a Thr I Phe G u Il e Ile I Al a Ser I Pro His 90 Hi s Thr G n Leu Page 516 Pr o SIle Asp Ser Pr o Val Al a Ar g Thr Asn Al a vet Ser Pr o Phe 110 Hi s Tr p Lys Asp SIe et Lys G u Val Lys G n 130 Al a Al a 145 Asp Val Al a Ser Arg Ala 115 Lys y Thr Tyr Pro 135 G u Ala Phe Leu Arg 150 Val Asp G u Val Leu 165 His Val Asp Asp Ile 180 Asn Ala Leu I e Asn 195 Gene Sequence 24-2-06.txt 120 125 Ala Val Tyr Asn Ala Ala Asn Gu G u 140 Sy Arg lie Lys Phe Pro n lie Val 155 160 Sn G y Al a Ser G n Phe Al a y Val 170 175 Leu Ala Thr u Ser G u Ala Arg Ala 185 190 Arg Leu Ala Thr Asn Leu 200 205 <210> 403 <211> 2031 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(2008) <223> RXN01562 <400> 403 gcaggtgcac atttgttttg tcacctgcac aaaagtgtcg ccagcccgat acttgtacaa ccgtccgcat ccgagaagca aaggtgtctg actcgcgcca atg gga att ctg aac 11! IVbt Gy Ile Leu Asn agt Ser gac Asp gca Al a at c SIle gat Asp aaa Lys tgc Cys gcg Al a t ca Ser ctt Leu ggt Sy ctt Leu tct Ser ttt Phe gct Al a ttg Leu 120 cct Pr o aaa Lys cac Hi s cga Ar g cag G n tct Ser agt Ser t at Tyr gct Al a gaa G u tta Leu gtt Val t cc Ser 75 tt g Leu gag G u gcg Al a gac Asp at c SIle ggt G y ttc Phe t at Tyr cgt Ar g cac Hi s gat Asp tta Leu cga Ar g cca Pr o 45 gat Asp gt g Val caa G n gat Asp ggt 15y 125 aag Lys act Thr aat Asn tcg Ser cat Hi s aaa Lys tgg Tr p 110 ttg Leu gcc Al a 15 ttc Phe ttg Leu cct Pr o aag Lys gat Asp 95 act Thr tct Ser ctt Leu ct g Leu ggc G y caa G n at c I e 80 ggc G y gag G u aaa Lys 1 aat Asn gt c Val gt a Val gac Asp ct g Leu ctt Leu tct Ser gcc Al a gat Asp gat Asp gt g Val ccg Pr o acg Thr tct Ser tcg Ser aag Lys 130 gag G u aaa Lys gaa G u at c SIle ggt G y ggt G y cat Hi s 115 cag G n gat Asp gt c Val tta Leu at c SIle cgc Ar g t ac Tyr 100 gct Al a ttg Leu ttg Leu gca Al a acc Thr ttt Phe gct Al a acc Thr tcg Ser gat Asp ggc gat acc acg cat agt gtg gtt gct gtc gtt ggt gat ggc gct cta Page 517 Sy Asp 135 act ggc Thr G y 150 gac cgc Asp Arg cca acc Pro Thr cct ttc Pro Phe atg ggg lVet y 215 gaa ggt G u Gy 230 ggc at g G y ltt gac aat Asp Asn cac at g Hi s Met ttg gac Leu Asp 295 gct cct Ala Pro 310 gag ctg G u Leu gcc gcg Al a Al a ccc aac Pro Asn tct gcc Ser Al a 375 tac t cc Tyr Ser 390 Thr Thr His Ser Val Gene Sequence 24-2-06.txt Val Ala Val Val y Asp Gy Ala Leu ggc G y aaa Lys at t SIle t at Tyr 200 tgg Tr p gt g Val aaa Lys gcg Al a gt c Val 280 gaa G u aaa Lys gt c Val at g Net cga Ar g 360 gca Al a acg Thr tgt Cys gtt Val 170 gga Gy cgc Ar g ggg Gy agc Ser gt g Val 250 aaa Lys gaa G u at g Met gca Al a at t SIle 330 ggt Gy ttt Phe ct c Leu ttg Leu tgg Tr p 155 gt c Val ttt Phe tt c Phe gag acc Thr 235 ggt G y tac Tyr aag Lys cac Hi s t ca Ser 315 ggt Gy cct Pr o gat Asp gca Al a aac Asn 395 140 gaa G u gt a Val gcg Al a at g Net cgt Ar g 220 gt c Val ccg Pr o gct Al a ggt G y t cc Ser 300 aag Lys gcg Al a acc Thr gt c Val ttg Leu 380 cgc Ar g gca Al a gt c Val gaa G u gaa G u 205 act Thr at t SIle gtt Val cat Hi s cgt Ar g 285 acg Thr ccc Pr o cag G n ggt Gy ggc Gy 365 ggt Gy gct Al a ct g Leu aat Asn aac Asn 190 aag Lys ttt Phe ccc Pr o gat Asp gat Asp 270 ggt G y ggc G y ggt G y aat Asn ct g Leu 350 at t SIle gga Gy ttt Phe aac Asn gac Asp 175 ctt Leu ggc Gy gaa G u acc Thr gga G y 255 t at Tyr t ac Tyr gt c Val tgg Tr p gaa G u 335 t cc Ser gct Al a aaa Lys gat Asp aat Asn 160 aat Asn gcg Al a aag Lys gcg Al a gaa G u 240 cat Hi s gat Asp gcg Al a at c SIle acc Thr 320 aac Asn aag Lys gag G u cac Hi s cag G n 400 gct Al a cgg Ar g ctt Leu t cc Ser 210 cat Hi s ttc Phe caa G n ccc Pr o gct Al a 290 ccg Pr o gt g Val gtt Val gaa G u cac Hi s 370 gt g Val ct c Leu gct Al a agt Ser cgc Ar g 195 ct g Leu gca Al a cct Pr o aaa Lys at c SIle 275 gag G u ct c Leu ttc Phe gcc Al a gcc Al a 355 gcg Al a gt g Val at g let ggt G y t at Tyr 180 at g Net aaa Lys ttt Phe gaa G u gct Al a 260 at c SIle cag G n aca Thr agc Ser at c l Ie 340 aat Asn gt a Val gct Al a gat Asp aaa Lys 165 tct Ser cag G n t cc Ser aaa Lys ct g Leu 245 gt c Val gt g Val gat Asp gga G y gat Asp 325 acc Thr ttc Phe act Thr at t SIe gt g Val 405 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 ggc atg ctc aac cag cct gtt act ttg gtg ctt gat cgc tca ggt gtc Page 518 Sy Met Leu Asn G n Pro Val 410 Gene Sequence 24- 2- 06. txt Thr Leu Val Leu Asp Arg Ser Gy Val 415 420 acg Thr acc Thr t cc Ser aca Thr 470 at c SIle act Thr gt a Val cag G n at c SIle 550 acc Thr gat Asp gt g Val gat Asp ct g Leu 630 tcg Ser at c SIle 440 cgt Ar g gt g Val acc Thr gtt Val gag G u 520 ggc G y cag G n gaa G u ctt Leu cag G n 600 ggc Gy t cc Ser gga G y cca Pr o ct g Leu ttc Phe gaa G u 490 t ca Ser gca Al a aac Asn ttg Leu ggc G y 570 gcc Al a t ac Tyr gac Asp gcg Al a ggc Gy ct c Leu ccc Pr o 475 gac Asp acc Thr act Thr gt c Val gt c Val 555 gt c Val tct Ser ct g Leu gcc Al a agc Ser gt g Val aat Asn 460 aag Lys ggc Gy gac Asp gtt Val acg Thr 540 gcg Al a at c SIle gag G u gat Asp gac Asp 620 aat ggc Asn G y 430 gtg gcg Val Ala gct at t Ala I e gac ttg Asp Leu gat gtc Asp Val 495 gcg cca Ala Pro 510 ctt gac Leu Asp gtt gac Val Asp tct gat Ser Asp ggc ggc G y G y 575 gat acc Asp Thr 590 gcg tcc Al a Ser att gaa Ile G u gt c Val gca Al a t cc Ser cca Pr o 480 ct c Leu t ca Ser gtt Val ccc Pr o gat Asp 560 gt g Val cct Pr o cgc Ar g acc tgg gat Trp Asp cca cgt Pro Ar g 450 at c gat Ile Asp 465 act cca Thr Pro gca t at Al a Tyr gtt ctc Val Leu gct t cc Al a Ser 530 cgc tgg Arg Trp 545 cat gac His Asp gga t cc G y Ser cgc cga Arg Arg aat gaa Asn u 610 act gt t Thr Val at g et 435 gat Asp gat Asp at t SIle gaa G u at c SIle 515 agg Ar g at t SIle ct c Leu ttg Leu caa G n 595 gt g Val gtt Val gcg Al a gag G u ggc Gy gtt Val gac Asp 500 at t SIle at t SIle gt c Val gt g Val ct c Leu 580 at c SIle ct c Leu gga Gy ct g Leu gat Asp ccc Pr o gct Al a 485 gcc Al a gcg Al a aaa Lys ccc Pr o at c SIe 565 t ct Ser gcc Al a gcc Al a tgg Tr p 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2031 ctg ttc ggg gaa Leu Phe G y G u 635 taaaaccctg cttatcgacg ccg <210> 404 <211> 636 <212> PRT <213> Corynebacteri um gl ut am cum <400> 404 Iet G y Ile Leu Asn Ser I e Ser Thr Pro Ala Asp Leu Lys Ala Leu 1 5 10 Page 519 Gene Sequence 24-2-06.txt Asn Asp G u Leu Asp Ala Leu Ala Lys u lie Arg Val Val Asp Leu Leu Ser Al a Gy 145 I le Gy Gy Thr Leu 225 Met Asn Gy Pr o Asp 305 Ser Val Phe Lys Su I e Gy Gy Hi s 115 G n Gy Al a Ser Ar g 195 Leu Al a Pr o Lys SIle 275 G u Leu Phe Al a Al a 355 Al a Thr Phe Al a Thr Ser Asp Leu Lys 165 Ser G n Ser Lys Leu 245 Val Val Asp Gy Asp 325 Thr Phe Al a SIle Asp 70 Lys Cys Al a Gy Thr 150 Asp Pr o Pr o bet G u 230 Gy Asp Hi s Leu Al a 310 G u Al a Pr o Thr Gy Thr Asp Ar g Al a Asp 135 Gy Ar g Thr Phe Gy 215 Gy Met Asn et Asp 295 Pr o Leu Al a Asn Gy Hi s Hi s Asp G u 105 Ser Thr Met Val Gy 185 Asp Val Lys Tyr Leu 265 Thr Leu Ser Lys Al a 345 Phe Leu Val Ser 75 Leu G u Al a Ser Tr p 155 Val Phe Phe G u Thr 235 Gy Tyr Lys Hi s Ser 315 Gy Pr o Asp Gy Phe Tyr Ar g Hi s Asp Val 140 G u Val Al a et Ar g 220 Val Pr o Al a Gy Ser 300 Lys Al a Thr Val Pr o Asp Val G n Asp Gy 125 Val Al a Val G u G u 205 Thr SIle Val Hi s Ar g 285 Thr Pr o G n Gy Gy 365 Thr Phe Leu Asn Leu G y Ser Pro G n His Lys Ile Lys Asp G y Trp Thr G u 110 Leu Ser Lys Al a Val Val Leu Asn Asn 160 Asn Asp Asn 175 Asn Leu Al a 190 Lys G y Lys Phe G u Al a Pro Thr G u 240 Asp G y Hi s 255 Asp Tyr Asp 270 G y Tyr Al a Sy Val lle G y Trp Thr 320 Asn G u Asn 335 Leu Ser Lys 350 Ile Ala G u G n His Ala Val Thr Ser Ala Ala y Leu Ala Leu y y Lys His Page 520 Gene Sequence 24-2-06.txt Pr o 385 Leu Asp Tr p Pr o SIle 465 Thr Al a Val Al a Ar g 545 Hi s Gy Ar g Asn Thr 625 Val Net Ser let 435 Asp Asp SIle G u SIle 515 Ar g SIle Leu Leu G n 595 Val Val SIle Val 405 Val Leu Asp Pr o Al a 485 Al a Al a Lys Pr o SIle 565 Ser Al a Al a Tr p 375 Ser Net Gy Ser Leu 455 Val Asp Asp Gy Hi s 535 Pr o SIle Al a Pr o Tyr 615 Asp 380 Arg Ala Phe Asp Thr Phe Leu Asn 395 Leu Asn G n Pro 410 Ser Asp G y Al a 425 Ile Val Pro G y 440 Arg G u Leu Leu Val Arg Phe Pro 475 Thr Leu G u Asp 490 Val G u Ser Thr 505 G u Arg Ala Thr 520 Sy Val Asn Val G n Ser Leu Val 555 G u Asp G y Val 570 Leu Asn Ala Ser 585 G n Lys Tyr Leu 600 G y Leu Asp Al a Ser Leu Phe G y 635 Thr Hi s G n 445 G u Gy Val Asp Al a 525 Val Leu Hi s Val Hi s 605 Gy Leu Asn 430 Val Al a Asp Asp Al a 510 Leu Val Ser Gy Asp 590 Al a SIle Val 415 Gy Al a SIe Leu Val 495 Pr o Asp Asp Asp Gy 575 Thr Ser G u <210> 405 <211> 1061 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (1)..(1038) <223> FRXA01562 <400> 405 gag cag gat ttg gac gaa ttg atg cac tcc acg ggc gtc atc gat ccg G u G n Asp Leu Asp G u Leu let His Ser Thr y Val lie Asp Pro 1 5 10 ctc aca gga gct cct aaa tct gca tca aag ccc ggt tgg acc tct gtg Leu Thr y Ala Pro Lys Ser Ala Ser Lys Pro Gy Trp Thr Ser Val Page 521 Gene Sequence 24-2-06.txt 25 ttc agc gat gag ctg gtc aag att ggt gcg cag aat gaa aac gtt gtt 144 Phe Ser Asp G u Leu Val Lys lie Gy Ala n Asn G u Asn Val Val 40 gcc atc acc gcc gcg atg gca ggt cct acc ggt ctg tcc aag ttc gaa 192 Ala Ile Thr Ala Ala Mbt Ala y Pro Thr y Leu Ser Lys Phe G u 55 gcc aat ttc ccc aac cga ttc ttt gat gtc ggc att gct gag cag cac 240 Ala Asn Phe Pro Asn Arg Phe Phe Asp Val G y IIe Ala G u G n His 70 75 gcg gta act tct gcc gca ggc ctc gca ttg ggt gga aaa cac cct gtg 288 Ala Val Thr Ser Ala Al a G y Leu Ala Leu G y G y Lys His Pro Val 90 gtg gct att tac tcc acg ttc ttg aac cgc gct ttt gat cag ctg ctc 336 Val Ala lle Tyr Ser Thr Phe Leu Asn Arg Ala Phe Asp G n Leu Leu 100 105 110 atg gat gtg ggc atg ctc aac cag cct gtt act ttg gtg ctt gat cgc 384 IVet Asp Val G y IVet Leu Asn G n Pro Val Thr Leu Val Leu Asp Arg 115 120 125 tca ggt gtc acg ggt tcg gat gga gcg agc cac aat ggc gtc tgg gat 432 Ser G y Val Thr G y Ser Asp G y Ala Ser His Asn G y Val Trp Asp 130 135 140 atg gcg ctg acc tcg atc gtt cca ggc gtg cag gtg gcg gca cca cgt 480 IVet Ala Leu Thr Ser Ile Val Pro G y Val G n Val Al a Al a Pro Arg 145 150 155 160 gat gag gat tcc ttg cgt gag ctg ctc aat gag gct att tcc atc gat 528 Asp G u Asp Ser Leu Arg G u Leu Leu Asn G u Ala lle Ser Ile Asp 165 170 175 gat ggc ccc aca gtt gtg cgt ttc ccc aag ggc gac ttg cca act cca 576 Asp y Pro Thr Val Val Arg Phe Pro Lys y Asp Leu Pro Thr Pro 180 185 190 att gtt gct atc gac acc ttg gaa gac ggc gtg gat gtc ctc gca tat 624 Ile Val Ala Ile Asp Thr Leu G u Asp y Val Asp Val Leu Ala Tyr 195 200 205 gaa gac gcc act gac gtt gaa tca acc gac gat gcg cca tca gtt ctc 672 G u Asp Ala Thr Asp Val u Ser Thr Asp Asp Ala Pro Ser Val Leu 210 215 220 atc att gcg gta ggc gag cgc gca act gtt gca ctt gac gtt gct tcc 720 Ile Ile Ala Val Gy G u Arg Ala Thr Val Ala Leu Asp Val Ala Ser 225 230 235 240 agg att aaa cag cac ggc gtg aac gtc acg gtt gtt gac ccc cgc tgg 768 Arg lie Lys G n His G y Val Asn Val Thr Val Val Asp Pro Arg Trp 245 250 255 att gtc ccc atc ccg cag tcc ttg gtc gcg ctg tct gat gat cat gac 816 Ile Val Pro Ile Pro G n Ser Leu Val Ala Leu Ser Asp Asp His Asp 260 265 270 ctc gtg atc acc atc gaa gac ggc gtc atc cac ggc ggc gtg gga tcc 864 Leu Val IIe Thr IIe G u Asp G y Val IIe His G y G y Val G y Ser 275 280 285 ttg ctc tct gat gcg ctt aac gcc tct gag gtg gat acc cct cgc cga 912 Leu Leu Ser Asp Ala Leu Asn Ala Ser G u Val Asp Thr Pro Arg Arg Page 522 Gene Sequence 24-2-06.txt 290 295 300 caa atc gcc gtg ccc cag aag tac ctg gat cac gcg tcc cgc aat gaa Sn Ile Ala Val Pro G n Lys Tyr Leu Asp His Ala Ser Arg Asn G u 305 310 315 320 gtg ctc gcc gat tat ggc ctc gac gcc gac ggc att gaa acc act gtt Val Leu Ala Asp Tyr G y Leu Asp Ala Asp Gy lie G u Thr Thr Val 325 330 335 gtt gga tgg ctg gat tcc ctg ttc ggg gaa taaaaccctg cttatcgacg Val y Trp Leu Asp Ser Leu Phe Gy G u 340 345 ccg <210> 406 <211> 346 <212> PRT <213> Corynebacter i um gl ut ai cum 960 1008 1058 1061 <400> 406 G u G n Asp Leu 1 Leu Phe Al a Al a Al a Val Met Ser Met 145 Asp Asp SIle Su I e 225 Gy Asp Thr Phe Thr SIle Val 115 Val Leu Asp Pr o Al a 195 Al a Al a Asp 5 Pr o Leu Al a Asn Al a Ser vet Gy Ser Leu 165 Val Asp Asp Gy Ser Lys Al a Phe Gy Phe Asn Asp 135 Val G u Ar g Leu G u 215 Ar g Al a I e 40 Gy Phe Leu Leu G n 120 Gy Pr o Leu Phe G u 200 Ser Al a G u Leu et His Ser Gy Pr o Asp Al a Asn 105 Pr o Al a Gy Leu Pr o 185 Asp Thr Thr Ser 10 Lys Al a Thr Val Leu 90 Ar g Val Ser Val Asn 170 Lys Gy Asp Thr Pr o G n Gy Gy Gy Al a Thr Hi s G n 155 G u Gy Val Asp Gy Gy Asn Leu I le Gy Phe Leu Asn 140 Val Al a Asp Asp Al a 220 Val Tr p G u Ser Al a Lys Asp Val 125 Gy Al a l Ie Leu Val 205 Pr o Asp Ser Val Phe G n Pr o Leu Asp Tr p Pr o SIe 175 Thr Al a Val Val Ala Leu Asp Val Ala Ser 235 240 Page 523 Ar g SIle Leu Leu G n 305 Val Val Lys Pr o SIle 275 Ser Al a Al a Tr p Hi s 245 Pr o SIle Al a Pr o Tyr 325 Asp Val Ser Asp Asn 295 Lys Leu Leu Gene Sequence 24-2-06.txt Asn Val Thr Val Val Asp Pro 250 Leu Val Ala Leu Ser Asp Asp 265 270 G y Val I I e His G y G y Val 280 285 Ala Ser G u Val Asp Thr Pro 300 Tyr Leu Asp His Ala Ser Arg 315 Asp Al a Asp G y ile G u Thr 330 Phe G y G u 345 Ar g 255 Hi s Gy Ar g Asn Thr 335 <210> 407 <211> 703 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(703) <223> FRXA01705 <400> 407 gcaggtgcac atttgttttg tcacctgcac aaaagtgtcg ccgtccgcat ccgagaagca aaggtgtctg actcgcgcca ccagcccgat act t gtacaa agt Ser gac Asp gca Al a at c SIle gat Asp aaa Lys tgc Cys t ca Ser ctt Leu ggt Gy ctt Leu tct Ser ttt Phe gct Al a cct Pr o aaa Lys cac Hi s cga Ar g cag G n tct Ser agt Ser gct Al a gaa G u tta Leu gtt Val t cc Ser 75 tt g Leu gag G u gac Asp at c SIle ggt Gy ttc Phe t at Tyr cgt Ar g cac Hi s tta Leu cga Ar g cca Pr o 45 gat Asp gt g Val caa G n gat Asp aag Lys act Thr aat Asn tcg Ser cat Hi s aaa Lys tgg Tr p 110 gcc Al a 15 ttc Phe ttg Leu cct Pr o aag Lys gat Asp 95 act Thr ctt Leu ct g Leu ggc Gy caa G n at c I e ggc ggy gag G u at g Met 1 aat Asn gt c Val gt a Val gac Asp ct g Leu ctt Leu tct Ser gat Asp gat Asp gt g Val ccg Pr o acg Thr tct Ser tcg Ser gag G u aaa Lys gaa G u at c SIle ggt Gy ggt Gy cat Hi s 115 gat Asp gtc Val tta Leu at c SIe cgc Ar g t ac Tyr 100 gct Al a gga at t ctg G y II e Leu gcg gcc ttg tct tat gcg gat ggt ttg tct aaa gcc aag cag ttg gat Page 524 Gene Sequence 24- 2- 06. txt Ala Ala Leu Ser Tyr Al a Asp y Leu Ser Lys Ala Lys G n Leu Asp 120 125 130 ggc gat acc acg cat agt gtg gtt gct gtc gtt ggt gat ggc gct cta 547 Gy Asp Thr Thr His Ser Val Val Ala Val Val y Asp Gy Ala Leu 135 140 145 act ggc ggc atg tgt tgg gaa gca ctg aac aat att gct gct ggt aaa 595 Thr Gy Gy IVet Cys Trp u Ala Leu Asn Asn lle Ala Al a y Lys 150 155 160 165 gac cgc aaa gtt gtt gtc gta gtc aat gac aat ggc cgg agt tat tct 643 Asp Arg Lys Val Val Val Val Val Asn Asp Asn y Arg Ser Tyr Ser 170 175 180 cca acc att ggc gga ttt gcg gaa aac ctt gcg ggc ctt cgc atg cag 691 Pro Thr lie Gy y Phe Al a u Asn Leu Al a y Leu Arg IVet n 185 190 195 cct ttc tat gat 703 Pro Phe Tyr Asp 200 <210> 408 <211> 201 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 408 IVet G y Ile Leu Asn Ser I e Ser Thr Pro Ala Asp Leu Lys Ala Leu 1 5 10 Asn Asp G u Asp Leu Asp Ala Leu Ala Lys G u lie Arg Thr Phe Leu 25 Val Asp Lys Val Ala Al a Thr G y G y His Leu G y Pro Asn Leu G y 40 Val Val G u Leu Thr II e G y Leu His Arg Val Phe Asp Ser Pro O n 55 Asp Pro lie lie Phe Asp Thr Ser His n Ser Tyr Val His Lys Ile 70 75 Leu Thr y Arg Ala Lys Asp Phe Asp Ser Leu Arg G n Lys Asp y 90 Leu Ser G y Tyr Thr Cys Arg Ala G u Ser G u His Asp Trp Thr G u 100 105 110 Ser Ser His Ala Ser Al a Ala Leu Ser Tyr Ala Asp G y Leu Ser Lys 115 120 125 Ala Lys G n Leu Asp G y Asp Thr Thr His Ser Val Val Ala Val Val 130 135 140 Sy Asp y Ala Leu Thr y y IVet Cys Trp u Ala Leu Asn Asn 145 150 155 160 lie Al a Al a y Lys Asp Arg Lys Val Val Val Val Val Asn Asp Asn 165 170 175 Sy Arg Ser Tyr Ser Pro Thr Ile y y Phe Ala G u Asn Leu Ala 180 185 190 G y Leu Arg Iet G n Pro Phe Tyr Asp 195 200 Page 525 Gene Sequence 24-2-06.txt <210> 409 <211> 2241 <212> DNA <213> Corynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(2218) <223> RXN00879 <400> 409 caccccaaag cccaatccaa aagatgtatt ttctaacaaa atgctgtgcc cacacgctat tagtggcata atgt t gtgt t ct t accct ca cgct acaaat gtg act gct cgc Val Thr Ala Arg ttt Phe gat Asp at c SIle gat Asp act Thr gtt Val gaa G u aca Thr aag Lys t cc Ser 150 ct g Leu gcg Al a aat Asn aaa Lys cgt Ar g gct Al a cca Pr o ccg Pr o gaa G u cca Pr o 120 cct Pr o gaa G u act Thr at c l Ie ct c Leu gcc Al a ct g Leu caa G n cct Pr o cat Hi s ggc G y gaa G u gat Asp t ca Ser gat Asp 170 tct Ser gcc Al a cat Hi s ggt G y cgc Ar g cca Pr o 75 gt g Val acg Thr at t SIle ct c Leu gct Al a 155 aag Lys gt g Val gat Asp at t SIle gt g Val caa G n tcg Ser cac Hi s cag G n gat Asp ccc Pr o 140 gag G u t at Tyr cgt Ar g t ac Tyr gt c Val tta Leu gcc Al a gtt Val ggt G y gat Asp 110 at t SIle ggt Gy ggt Gy gat Asp acg Thr 190 ggc Gy 15 agc Ser gat Asp ct c Leu gca Al a t cc Ser 95 gtt Val agg Ar g tgg Tr p ttg Leu t cc Ser 175 ttg Leu gt a Val gat Asp aca Thr ttc Phe gtt Val 80 cct Pr o tct Ser tgg Tr p cac Hi s at c SIle 160 cct Pr o tcg Ser 1 gca Al a gac Asp agc Ser cat Hi s gaa G u gca Al a cag G n ggc G y aag Lys 145 at c SIle cgc Ar g tgg Tr p act Thr aca Thr aac Asn gat Asp ggt G y gat Asp gt g Val gag G u 130 ctt Leu acc Thr agt Ser ggc G y t cc Ser tta Leu ct c Leu cga Ar g gat Asp gt c Val gaa G u 115 gca Al a cac Hi s ccg Pr o ggt G y at g 1et 195 t ac Tyr gt g Val ccc Pr o gag G u gaa G u cac Hi s 100 aac Asn tcg Ser ctt Leu gct Al a gt c Val 180 ggt G y aga Ar g act Thr aaa Lys aac Asn ttc Phe ct a Leu at c SIe tgg Tr p ttt Phe aaa Lys cgt Ar g 165 at g let gat Asp ttc aat gat tta gga aac ttg gca agt gtg gtt gcc cag gat gga gca Page 526 Gene Sequence 24-2-06.txt Phe Asn Asp Leu y Asn Leu Ala Ser Val Val Ala G n Asp y Ala 200 205 210 gac ttc ctg ctc atc aac ccc atg cac gct gca gag ccg ctg cct cct 787 Asp Phe Leu Leu Ile Asn Pro IVet His Ala Ala G u Pro Leu Pro Pro 215 220 225 act gag gac tct cct tat ctg ccc aca acc agg cgc ttt atc aac ccg 835 Thr G u Asp Ser Pro Tyr Leu Pro Thr Thr Arg Arg Phe lie Asn Pro 230 235 240 245 atc tac att cgg gta gaa gat att ccg gag ttt aat cag ctt gag att 883 Ile Tyr Ile Arg Val u Asp lie Pro u Phe Asn n Leu u Ile 250 255 260 gat cta cgc gat gat atc gca gag atg gct gcg gaa ttc cgc gaa cgc 931 Asp Leu Arg Asp Asp Ile Ala G u Iet Ala Ala G u Phe Arg G u Arg 265 270 275 aat ctg acc tca gac atc att gag cgc aat gac gtc tac gct gca aag 979 Asn Leu Thr Ser Asp Ile lie G u Arg Asn Asp Val Tyr Ala Ala Lys 280 285 290 ctt caa gtg ctg cgc gcc att ttt gaa atg cct cgt tcc agc gaa cgt 1027 Leu G n Val Leu Arg Al a lie Phe G u IVet Pro Arg Ser Ser G u Arg 295 300 305 gaa gcc aac ttt gtc tcc ttc gtg caa cgg gaa ggc caa ggt ctt att 1075 Su Ala Asn Phe Val Ser Phe Val n Arg Gu Gy Gn Gy Leu Ile 310 315 320 325 gat ttc gcc acc tgg tgc gcg gac cgc gaa act gca cag tct gaa tct 1123 Asp Phe Ala Thr Trp Cys Ala Asp Arg G u Thr Ala G n Ser G u Ser 330 335 340 gtc cac gga act gag cca gac cgc gat gag ctg acc atg ttc tac atg 1171 Val Hi s y Thr G u Pro Asp Arg Asp G u Leu Thr Iet Phe Tyr IVet 345 350 355 tgg ttg cag tgg cta tgt gat gag cag ctg gcg gca gct caa aag cgc 1219 Trp Leu G n Trp Leu Cys Asp G u G n Leu Ala Ala Ala G n Lys Arg 360 365 370 gct gtc gat gcc gga atg tcg atc ggc atc atg gca gac ctg gca gtt 1267 Ala Val Asp Ala G y IVt Ser lie y lie IVt Ala Asp Leu Ala Val 375 380 385 ggt gtg cat cca ggt ggt gct gat gcc cag aac ctc agc cac gta ctt 1315 G y Val His Pro G y G y Ala Asp Ala G n Asn Leu Ser His Val Leu 390 395 400 405 gct ccg gat gcg tca gtg ggc gcc cca cca gat gga tac aac cag cag 1363 Ala Pro Asp Ala Ser Val G y Ala Pro Pro Asp y Tyr Asn G n n 410 415 420 ggc caa gac tgg tcc cag cca cca tgg cat cca gtg cgt ctt gca gag 1411 G y G n Asp Trp Ser G n Pro Pro Trp His Pro Val Arg Leu Ala G u 425 430 435 gaa ggc tac att ccg tgg cgt aat ctg ctg cgc act gtg ctg cgt cac 1459 G u y Tyr lie Pro Trp Arg Asn Leu Leu Arg Thr Val Leu Arg His 440 445 450 tcc ggc gga atc cgc gtg gac cac gtt ctt ggt ttg ttc agg ctc ttt 1507 Ser G y G y lie Arg Val Asp His Val Leu G y Leu Phe Arg Leu Phe 455 460 465 gtc atg cca cgc atg caa tcc cct gct acg ggc acc tat atc cgc ttc 1555 Page 527 Val MVt Pro Arg MVt G n Ser 470 475 gac cat aat gcg ttg gta ggc Asp His Asn Ala Leu Val y 490 ggc gcc gtt gtc att ggt gaa Sy Ala Val Val Ile y u 505 caa gat gca ttg gct cag cgt G n Asp Ala Leu Ala G n Arg 520 ttc gag cat tcc cca agc cag Phe G u His Ser Pro Ser G n 535 540 cgt ccg ctg gcc ttg acc act Arg Pro Leu Ala Leu Thr Thr 550 555 gct ggt tat ttg gag ggc gag Ala y Tyr Leu u y u 570 gtg ctc aac act gat cct gct Val Leu Asn Thr Asp Pro Ala 585 caa gcg gag atc ctt gat gtc Sn Al a G u Ile Leu Asp Val 600 cgg gaa tac gtg gga ctc gaa Arg G u Tyr Val y Leu G u 615 620 ctg ttg gaa ggc ctg cac act Leu Leu G u G y Leu His Thr 630 635 acc tgt gtc tgc ttg gta gac Thr Cys Val Cys Leu Val Asp 650 cag ccg ggc aca acg agg gat G n Pro y Thr Thr Arg Asp 665 tgt gac agc gaa ggc aac tcc Cys Asp Ser G u G y Asn Ser 680 gag ctg tat cac cgt gtg gca G u Leu Tyr His Arg Val Ala 695 700 tcagttgtgg tgg Gene Sequence 24-2-06.txt Pro Ala Thr Gy Thr Tyr I 480 I e Arg Phe 485 at t SIle gat Asp ggc Gy 525 ccg Pr o gt g Val cac Hi s gca Al a gca Al a 605 cgc Ar g ttc Phe at g bet at g Net gt g Val 685 ct a Leu ct g Leu 510 at c SIle ggt Gy acc Thr at t SIle gaa G u 590 gca Al a gat Asp gtt Val gt c Val t at Tyr 670 ct c Leu gcc Al a 495 gga Gy at g bet cct Pr o act Thr gct Al a 575 ct c Leu tct Ser cag G n gcg Al a ggt G y 655 ccc Pr o at t SIle ct a Leu acg Thr ggc Gy cgc Ar g cat Hi s 560 ctt Leu gct Al a gcc Al a cgc Ar g aaa Lys 640 gaa G u aac Asn gaa G u gaa G u ttt Phe acc Thr cgc Ar g 545 gat Asp cgt Ar g gag G u aac Asn ggt G y 625 acc Thr aag Lys tgg Tr p tcg Ser gca Al a gag G u tcg Ser 530 cag G n ct c Leu gag G u gat Asp gca Al a 610 gag G u cct Pr o cgg Ar g tgt Cys ct g Leu 690 gaa G u cct Pr o 515 at c SIle gaa G u cct Pr o cga Ar g ct g Leu 595 ttg Leu ttg Leu t ca Ser gca Al a at c SIle 675 cgt Ar g ct c Leu 500 tgg Tr p ct a Leu gag G u ccg Pr o ttg Leu 580 cag G n cca Pr o gct Al a gca Al a cag G n 660 cca Pr o gaa G u gca Al a gt a Val tgg Tr p t at Tyr act Thr 565 ggg G y tgg Tr p gcc Al a gag G u ct g Leu 645 aat Asn ct g Leu aat Asn 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 2228 2241 aag gca agc aag Lys Ala Ser Lys cga gat Arg Asp 705 t aggt ccgct <210> 410 <211> 706 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 410 Page 528 Val Thr Ala Arg Arg 1 5 Gene Sequence 24- 2- 06. txt Phe Leu Asn G u Leu Ala Asp Leu Tyr G y Val Al a Asp Ser Hi s G u Al a G n Gy Lys 145 I le Ar g Tr p Al a G u 225 Ar g Asn G u Val Ar g 305 Gy Al a Thr Ser Leu Leu Ar g Asp Val G u 115 Al a Hi s Pr o Gy Met 195 Asp Leu SIle Leu Ar g 275 Al a Ser Gy Ser Phe 355 Thr Lys Asn Phe Leu I le Tr p Phe Lys Ar g 165 let Asp Al a Pr o Pr o 245 I le Ar g Lys Ar g SIle 325 Ser \et Tyr Leu Asp Ar g Phe Leu Al a SIle 135 Asn Ser G n Asn Phe 215 G u Tyr Leu Leu G n 295 Al a Phe Hi s Leu Lys Ar g 40 Al a Pr o Pr o G u Pr o 120 Pr o G u Thr SIle Asp 200 Leu Asp SIle Ar g Thr 280 Val Asn Al a Gy G n 360 Gy Al a l Ie Leu Val Asp 105 Ar g Gy Ar g Al a Tyr 185 Leu Leu Ser Ar g Asp 265 Ser Leu Phe Thr Thr 345 Tr p Al a Leu G n Pr o Hi s 90 Gy G u Asp Ser Asp 170 Ser Gy I e Pr o Val 250 Asp Asp Ar g Val Tr p 330 G u Hi s Gy Ar g Pr o Val Thr SIle Leu Al a 155 Lys Val Asn Asn Tyr 235 G u SIle SIle Al a Ser 315 Cys Pr o SIle Val G n Ser Hi s G n Asp Pr o 140 G u Tyr Ar g Leu Pr o 220 Leu Asp Al a SIle l Ie 300 Phe Al a Asp G u Asn I le Val Asp Ar g Sy 125 Leu Cys Leu Ser Al a 205 let Pr o SIle G u G u 285 Phe Val Asp Ar g Ser Asp Leu Al a Ser Val Ar g Tr p Leu Ser 175 Leu Val Al a Thr G u 255 Al a Asn vet Ar g G u 335 G u Leu Cys Asp G u n Leu Ala 365 Page 529 Gene Sequence 24-2-06.txt Al a Al a 385 Leu Gy Val Thr Leu 465 Thr G u Phe Thr Ar g 545 Asp Ar g G u Asn Gy 625 Thr Lys Tr p Ser Ar g 705 G n Leu Hi s Asn Leu 435 Leu Ar g SIle G u Pr o 515 I le G u Pr o Ar g Leu 595 Leu Leu Ser Al a SIle 675 Ar g Al a Val 375 Val G y Val 390 Leu Ala Pro 405 Gn G y G n G u G u G y Hi s Ser G y 455 Phe Val Vbet 470 Phe Asp Hi s 485 Al a G y Al a Val G n Asp Trp Phe G u 535 Tyr Arg Pro 550 Thr Al a G y 565 G y Val Leu Trp G n Ala Al a Ar g G u 615 G u Leu Leu 630 Leu Thr Cys 645 Asn n Pro Leu Cys Asp Asp Al a G y IVet Ser lie y lie IVet Pr o Al a Tr p 425 I le SIle Ar g Al a Val 505 Leu Ser Al a Leu Thr 585 l le Val Sy Cys Thr 665 G u Sy 395 Val G n Tr p Val G n 475 Val Gy G n Ser Thr 555 Sy Pr o Asp Leu Hi s 635 Val Ar g Asn 380 Al a Sy Pr o Ar g Asp 460 Ser Sy G u Ar g G n 540 Thr G u Al a Val G u 620 Thr Asp Asp Ser Asp Al a Pr o Asn 445 Hi s Pr o SIle Asp Sy 525 Pr o Val Hi s Al a Al a 605 Ar g Phe bet vet Val 685 Al a Pr o Tr p 430 Leu Val Al a Leu Leu 510 I le Sy Thr SIle G u 590 Al a Asp Val Val Tyr 670 Leu G n Pr o 415 Hi s Leu Leu Thr Al a 495 Gy vet Pr o Thr Al a 575 Leu Ser G n Al a Sy 655 Pr o SIe Arg u Asn u Leu 695 Tyr His Arg Val Ala Lys Ala Ser Lys 700 Page 530 Gene Sequence 24-2-06.txt <210> 411 <211> 2223 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(2200) <223> FRXA00879 <400> 411 aaaagatgta ttttctaaca aacttaccct cacgctacaa attagtggca taatgttgtg ttgtgactgc tcgcagattt at atgctgtg cccacacgct aat gaa ct c Asn G u Leu gat Asp at t SIle gt g Val caa G n tcg Ser cac Hi s cag G n gat Asp ccc Pr o gag G u 150 t at Tyr cgt Ar g ttg Leu t ac Tyr gt c Val tta Leu gcc Al a gtt Val ggt Gy gat Asp at t SIle 120 ggt Sy ggt Sy gat Asp acg Thr agt Ser 200 gt a Val gat Asp aca Thr ttc Phe gtt Val cct Pr o tct Ser tgg Tr p cac Hi s at c SIle cct Pr o 170 tcg Ser gtt Val gca Al a gac Asp agc Ser cat Hi s gaa G u 75 gca Al a cag G n ggc G y aag Lys at c SIle 155 cgc Ar g tgg Tr p gcc Al a act Thr aca Thr aac Asn gat Asp ggt Gy gat Asp gt g Val gag G u ctt Leu 140 acc Thr agt Ser ggc G y cag G n t cc Ser tta Leu ct c Leu 45 cga Ar g gat Asp gt c Val gaa G u gca Al a 125 cac Hi s ccg Pr o ggt G y at g let gat Asp 205 t ac Tyr gt g Val ccc Pr o gag G u gaa G u cac Hi s aac Asn 110 tcg Ser ctt Leu gct Al a gt c Val ggt G y 190 gga Gy act Thr 15 aaa Lys aac Asn ttc Phe ct a Leu at c SIle 95 tgg Tr p ttt Phe aaa Lys cgt Ar g at g Met 175 gat Asp gca Al a gat Asp at c SIle gat Asp act Thr gtt Val 80 gaa G u aca Thr aag Lys t cc Ser ct g Leu 160 gcg Al a ttc Phe gac Asp aaa Lys cgt Ar g gct Al a cca Pr o ccg Pr o gaa G u cca Pr o cct Pr o 130 gaa G u act Thr at c l Ie gat Asp ct g Leu 210 ggt G y gct Al a at c l Ie ct g Leu gt g Val gac Asp cgg Ar g 115 ggt G y cgc Ar g gct Al a t ac Tyr tta Leu 195 ct c Leu gcc Al a ct g Leu caa G n cct Pr o cat Hi s ggc Gy 100 gaa G u gat Asp t ca Ser gat Asp tct Ser 180 gga G y at c SIle gcc Al a cat Hi s ggt G y cgc Ar g cca Pr o gtg Val acg Thr att SIe ct c Leu gct Al a aag Lys 165 gtg Val aac Asn aac Asn Page 531 Gene Sequence 24-2-06.txt ccc Pr o ct g Leu 230 gat Asp gca Al a at t SIle at t SIle ttc Phe 310 gcg Al a gac Asp gat Asp tcg Ser gct Al a 390 ggc G y cca Pr o cgt Ar g gac Asp t cc Ser 470 cac Hi s aca Thr ccg Pr o at g bet cgc Ar g 280 gaa G u caa G n cgc Ar g gat Asp cag G n 360 ggc Gy gcc Al a cca Pr o tgg Tr p ct g Leu 440 gtt Val gct Al a gca Al a agg Ar g ttt Phe 250 gcg Al a gac Asp cct Pr o gaa G u act Thr 330 ct g Leu gcg Al a at g Net aac Asn gat Asp 410 cca Pr o cgc Ar g ggt Sy ggc G y gag G u cgc Ar g 235 aat Asn gaa G u gt c Val cgt Ar g ggc Gy 315 gca Al a acc Thr gca Al a gca Al a ct c Leu 395 gga G y gt g Val act Thr tt g Leu acc Thr 475 ccg ct g cct Pro Leu Pro 220 ttt atc aac Phe Ile Asn cag ctt gag G n Leu G u ttc cgc gaa Phe Ar g G u 270 t ac gct gca Tyr Al a Al a 285 t cc agc gaa Ser Ser G u 300 caa ggt ct t Sn G y Leu cag t ct gaa G n Ser G u atg ttc tac IVet Phe Tyr 350 gct caa aag Al a G n Lys 365 gac ctg gca Asp Leu Al a 380 agc cac gt a Ser Hi s Val tac aac cag Tyr Asn G n cgt ctt gca Arg Leu Ala 430 gtg ctg cgt Val Leu Arg 445 ttc agg ctc Phe Arg Leu 460 tat atc cgc Tyr I e Ar g cct act gag gac Pro Thr G u Asp 225 ccg atc tac att Pro Ile Tyr Ile 240 att gat cta cgc Ile Asp Leu Arg 255 cgc aat ctg acc Arg Asn Leu Thr aag ctt caa gtg Lys Leu G n Val 290 cgt gaa gcc aac Arg G u Ala Asn 305 att gat ttc gcc Ile Asp Phe Al a 320 tct gtc cac gga Ser Val His G y 335 atg tgg ttg cag IVet Trp Leu G n cgc gct gtc gat Arg Ala Val Asp 370 gtt ggt gtg cat Val G y Val Hi s 385 ctt gct ccg gat Leu Ala Pro Asp 400 cag ggc caa gac Gn G y n Asp 415 gag gaa ggc tac G u G u G y Tyr cac tcc ggc gga Hi s Ser G y G y 450 ttt gtc atg cca Phe Val et Pro 465 ttc gac cat aat Phe Asp His Asn 480 Page 532 tct cct tat Ser Pro Tyr cgg Ar g gat Asp t ca Ser 275 ct g Leu ttt Phe acc Thr act Thr tgg Tr p 355 gcc Al a cca Pr o gcg Al a tgg Tr p at t SIle 435 at c SIle cgc Ar g gcg Al a gt a Val gat Asp 260 gac Asp cgc Ar g gt c Val tgg Tr p gag G u 340 ct a Leu gga Gy ggt Gy t ca Ser t cc Ser 420 ccg Pr o cgc Ar g at g bet ttg Leu 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 Gene Sequence 24-2-06.txt ggc Gy gaa G u cgt Ar g cag G n act Thr 550 gag G u gct Al a gt c Val gaa G u act Thr 630 gac Asp gat Asp t cc Ser gca Al a ct a Leu ct g Leu at c SIle 520 ggt Sy acc Thr at t SIle gaa G u gca Al a 600 gat Asp gtt Val gt c Val t at Tyr ct c Leu 680 gca Al a ct a Leu 490 acg Thr ggc G y cgc Ar g cat Hi s ctt Leu 570 gct Al a gcc Al a cgc Ar g aaa Lys gaa G u 650 aac Asn gaa G u gca Al a gag G u tcg Ser cag G n 540 ct c Leu gag G u gat Asp gca Al a gag G u 620 cct Pr o cgg Ar g tgt Cys ct g Leu gat Asp 700 gaa ctc gca ggc gcc gtt U u Leu cct Pr o at c SIle 525 gaa G u cct Pr o cga Ar g ct g Leu ttg Leu 605 ttg Leu t ca Ser gca Al a at c SIle cgt Ar g 685 tgg Tr p 510 ct a Leu gag G u ccg Pr o ttg Leu cag G n 590 cca Pr o gct Al a gca Al a cag G n cca Pr o 670 gaa G u AI a 495 gt a Val tgg Tr p t at Tyr act Thr ggg G y 575 tgg Tr p gcc Al a gag G u ct g Leu aat Asn 655 ct g Leu aat Asn u y AI a val caa G n ttc Phe cgt Ar g gct Al a 560 gt g Val caa G n cgg Ar g ct g Leu acc Thr 640 cag G n tgt Cys gag G u gat Asp gag G u ccg Pr o 545 ggt G y ct c Leu gcg Al a gaa G u ttg Leu 625 tgt Cys ccg Pr o gac Asp ct g Leu gca Al a cat Hi s 530 ct g Leu t at Tyr aac Asn gag G u t ac Tyr 610 gaa G u gt c Val ggc G y agc Ser t at Tyr 690 gtc att Val lie 500 ttg gct Leu Al a 515 t cc cca Ser Pro gcc ttg Al a Leu ttg gag Leu G u act gat Thr Asp 580 atc ctt I e Leu 595 gtg gga Val G y ggc ctg G y Leu tgc ttg Cys Leu aca acg Thr Thr 660 gaa ggc Su G y 675 cac cgt His Arg 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 2223 agc aag cga Ser Lys Ar g taggtccgct tcagttgtgg tgg <210> 412 <211> 700 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 412 Leu Asn G u Leu Ala Asp Leu Tyr y Val Ala Thr Ser Tyr Thr Asp 1 5 10 Tyr Lys G y Ala His I e G u Val Ser Asp Asp Thr Leu Val Lys Ile Page 533 Gene Sequence 24-2-06.txt Asp Thr Ser Pro Asn Asp Leu Asp Ar g Phe Leu Al a SIle Asn 145 Ser G n Asn Phe G u 225 Tyr Leu Leu G n Al a 305 Phe Hi s Leu Val Al a I e Leu Val Asp Ar g 115 Gy Ar g Al a Tyr Leu 195 Leu Ser Ar g Asp Ser 275 Leu Phe Thr Thr Tr p 355 Al a Gy Ar g Pr o Val Thr SIle Leu Al a Lys 165 Val Asn Asn Tyr G u 245 I e I e Al a Ser Cys 325 Pr o Cys let Asn I e Val Asp Ar g Gy Leu 135 Cys Leu Ser Al a let 215 Pr o SIle G u G u Phe 295 Val Asp Ar g G u SIle 375 Leu 40 Al a Val Gy Asp SIle 120 Gy Gy Asp Thr Ser 200 Hi s Thr Pr o let Ar g 280 G u G n Ar g Asp G n 360 Gy Leu Al a Ser Val 105 Ar g Tr p Leu Ser Leu 185 Val Al a Thr G u Al a 265 Asn let Ar g G u G u 345 Leu SIle Phe Val Pr o 90 Ser Tr p Hi s SIle Pr o 170 Ser Val Al a Ar g Phe 250 Al a Asp Pr o G u Thr 330 Leu Al a Hi s G u Al a G n Gy Lys SIle 155 Ar g Tr p Al a G u Ar g 235 Asn G u Val Ar g Gy 315 Al a Thr Al a Asn Leu Asp Arg Sy Asp Asp Val Val G u G u Al a 125 Leu Hi s 140 Thr Pro Ser G y G y let Sn Asp 205 Pro Leu 220 Phe Il e G n Leu Phe Arg Tyr Al a 285 Ser Ser 300 G n y G n Ser let Phe Al a G n 365 G u Q u Hi s Asn 110 Ser Leu Al a Val Sy 190 Sy Pr o Asn G u G u 270 Al a G u Leu G u Tyr 350 Lys Al a Phe Leu SIe Tr p Phe Lys Ar g let 175 Asp Al a Pr o Pr o SIe 255 Ar g Lys Ar g SIe Ser 335 let Ar g Val IVet Al a Asp 380 Leu Page 534 Gene Sequence 24- 2- 06. txt Ala G n Asn Leu Ser His Val Leu Ala Val His Pro y y Ala Asp 385 390 Pr o G n Sy Sy let 465 Hi s Al a Asp G u Pr o 545 Sy Leu Al a G u Leu 625 Cys Pr o Asp Leu Al a Tr p SIle 435 I le Ar g Al a Val Leu 515 Ser Al a Leu Thr l Ie 595 Val Sy Cys Thr G u 675 Hi s Val 405 G n Tr p Val G n Val 485 Sy G n Ser Thr Gy 565 Pr o Asp Leu Hi s Val 645 Ar g Asn Gy Pr o Ar g Asp Ser 470 Gy G u Ar g G n Thr 550 G u Al a Val G u Thr 630 Asp Asp Ser Al a Pr o Asn Hi s 455 Pr o SIle Asp Sy Pr o 535 Val Hi s Al a Al a Ar g 615 Phe bet let Val Lys 695 Pr o Hi s 425 Leu Leu Thr Al a Sy 505 let Pr o Thr Al a Leu 585 Ser G n Al a Sy Pr o 665 I le Asp 410 Pr o Ar g Sy Sy Leu 490 Thr Sy Ar g Hi s Leu 570 Al a Al a Ar g Lys G u 650 Asn G u 395 Sy Val Thr Leu Thr 475 G u Phe Thr Ar g Asp 555 Ar g G u Asn Sy Thr 635 Lys Tr p Ser Asn Leu Leu 445 Ar g SIle G u Pr o SIle 525 G u Pr o Ar g Leu Leu 605 Leu Ser Al a SIle Ar g 685 G n Al a 430 Ar g Leu Ar g Leu Tr p 510 Leu G u Pr o Leu G n 590 Pr o Al a Al a G n Pr o 670 G u G n 415 G u Hi s Phe Phe Al a 495 Val Tr p Tyr Thr Sy 575 Tr p Al a G u Leu Asn 655 Leu Asn 400 Sy G u Ser Val Asp 480 Sy G n Phe Ar g Al a 560 Val G n Ar g Leu Thr 640 G n Cys G u Arg Val Ala Ala Ser Lys Arg <210> 413 <211> 1287 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS Page 535 Gene Sequence 24-2-06.txt <222> (101)..(1264) <223> RXN00043 <400> 413 aacagcaggc ctcaagtccg aagataatta acctaaatcc gtcctatgct tgctggaagg aagcaaataa cctcagaaag gtagacataa gacatcatac atg gca gaa gtg gtg 11! lMt Ala Gu Val Val cat Hi s at t l Ie ggc Gy ttc Phe aat Asn gcg Al a ttg Leu gaa G u ct c Leu gat Asp 150 gcg Al a aat Asn t cc Ser gcc Al a aat Asn caa G n acc Thr at c SIle ccc Pr o ggt Gy aat Asn agc Ser ctt Leu 120 ggt Gy at t SIle aaa Lys act Thr ggg Gy 200 gct Al a at g let aat Asn cac Hi s gaa G u ct c Leu aac Asn gcg Al a gtt Val ccc Pr o ttc Phe ccc Pr o tgg Tr p 170 ctt Leu act Thr gag G u ccg Pr o gca Al a ggg Gy ct c Leu ccc Pr o ggt Gy 75 cag G n tcg Ser tt g Leu at c SIle ggc Gy 155 at c SIle ct c Leu gat Asp aaa Lys ct g ggt Gy gt g Val tct Ser acg Thr ggc Gy t at Tyr gcg Al a tgt Cys aac Asn 140 aac Asn aaa Lys gat Asp gca Al a aat Asn 220 cat caa G n at t SIle gga Gy 45 at t SIle gcg Al a cac Hi s ccg Pr o gaa G u 125 gca Al a cca Pr o tcg Ser ct c Leu gat Asp 205 gt g Val cat gca gtt Al a Val 15 gat ggc Asp y gaa cca G u Pro gtt ccc Val Pr o ttt cct Phe Pro cgc gaa Arg G u 95 gct gac Al a Asp 110 gag ggc G u G y tgc cgt Cys Arg aca gat Thr Asp atc aca Ile Thr 175 tgc gca Cys Ala 190 ttt gat Phe Asp acg gtc Thr Val agg gat Arg Asp aaa Lys ttt Phe gca Al a agt Ser acg Thr cat Hi s gca Al a ct g Leu tgt Cys ctt Leu 160 gt a Val gcg Al a acc Thr acg Thr 1 aaa Lys ct c Leu cct Pr o ttt Phe gga Gy ggc Gy ct g Leu ct g Leu ggt Gy 145 gcc Al a gcg Al a cac Hi s act Thr gct Al a 225 at t SIle caa G n aaa Lys at t SIle acg Thr acg Thr gca Al a tgc Cys 130 gct Al a cag G n ccg Pr o cac Hi s acc Thr 210 acg Thr gaa G u ct c Leu aac Asn gat Asp cag G n acc Thr gcg Al a 115 ggc G y caa G n gt g Val gaa G u at c SIle 195 agc Ser cat Hi s gga Sy gaa G u gca Al a ctt Leu gac Asp gtg Val 100 cag G n att SIe aac Asn at c SIe act Thr 180 att SIe gca Al a ttg Leu ccc ggc agc gtg ggc gct Pro y Ser Val Oy Ala Leu Hi s Hi s Page 536 ene Sequence 24- 2- 06. txt 230 t tg ct t Leu Leu gac ggc As p (1y aac gcc As n A a ggt gag O31y (1u 295 gcc cgt Ala Ar g 310 gcg agt A a Ser gcg acc A a Thr gat cac As p Hi s tca aac Ser As n 375 gct A a gt g ValI tt t Ph e 280 t ac Tyr ct g Le u c ag O31n ct c Le u g aa O31u 360 ggc G31y gca A a 250 tt g Le u at c I Ie tt g Le u g at As p gt g ValI 330 ac c Thr gt t ValI t ta cgt Ar g gcc A a ac g Thr ggc G31y ggc G31y 315 cac Hi s t ca Ser aaa Ly s caa gcc A a g at As p g ac As p gt t ValI 300 ggc G31y c ac Hi s ac c Thr t cc Ser c ag O31n 380 ggg O31y gga O31y gcc A a 285 tt g Le u gcc A a gt g ValI gt c ValI aac As n 365 g ac As p ac g Thr 270 at g vb t aac As n at c I Ie cgc Ar g gcc A a 350 cct Pr o gca A a 255 gt c ValI g aa O31u gt c ValI gcc A a ag g Ar g 335 gcc A a gt a ValI 240 t at Tyr g at As p gcc A a ac c Thr ggg O31y 320 ggt O31y aaa Ly s aat As n gt t ValI ct a Le u gcc A a gt c ValI 305 ggt O31y at g vb t at t I Ie tt t Ph e gag O31u gct A a gga O31y 290 ac c Thr ac c Thr ac g Thr ct c Le u gt g ValI 370 at c I Ie 260 t cc Ser cca Pr o ggc O31y ac a Thr at c I Ie 340 ct t Le u tt t Ph e 883 931 979 1 027 1 075 1123 1171 1219 1264 1287 03 n Leu 03 n gtc cat tta gac Val Hi s Leu Asp cat caa gt a at t His On Val Ilie 385 t aaat acgag caaaact t tc ct g <210O> 414 <21 1> 388 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 414 I'b t Ala Ou Val Val His Tyr On Ou 1 5 Lys I Ie O31u 31y Ar g I Ie ValI Thr Pr o 25 Leu 03 n Leu 03 u As n 03 y I I e I I e Thr 40 Pr o Lys Asn Ala O31y Ph e Hi s Pr o O31u 55 Phe I I e As p Leu Hi s As n Hi s 03 y 03 y 70 O31y Thr O31n As p O31n Ala Ar g As n Ala O31y Thr Thr Val Ntbt Leu Ala Ser Nib t 100 105 As n A a 03 y 10 Hi s 03 y Val1 O3 u Leu Ser Leu Pro Thr Asn (1y (1y A a 03 n Tyr 90 Val Ser A a Page 537 O31n I Ie O31y li1e A a Hi s Pr o A a As p O31u ValI Ph e Ar g A a 110 ValI O31y Pr o Pr o Pr o O31u As p Leu Ala Ala n Val u Asn 115 Gene Sequence 24-2-06. t xt Leu Ile Pro Leu Cys G u G u G y Leu Leu Gy 145 Al a Al a Hi s Thr Al a 225 Gy Val Leu Al a Val 305 Gy Met I le Phe Hi s 385 Gy G n Val G u SIle 195 Ser Hi s Val Leu Ar g 275 vbt Asp Ser Leu Sy 355 Val Val Hi s Pr o Hi s 165 Asp Al a SIle Phe Al a 245 Al a Asn Asp Val Leu 325 Asp Ser Asp Leu Asp 150 Al a Asn Ser Al a Asn 230 Leu Asp Asn Gy Al a 310 Al a Al a Asp Ser G u 135 Phe Gy Leu Phe Leu 215 Al a Leu Gy Al a G u 295 Ar g Ser Thr Hi s Asn 375 120 Gy I e Lys Thr Gy 200 Al a bet Al a Val Phe 280 Tyr Leu G n Leu G u 360 Gy 125 Pr o Phe Gy G u 185 Hi s Lys Pr o Al a Hi s 265 Phe SIle Ar g Phe Hi s 345 I le G n Phe Pr o Tr p 170 Leu Thr G u Pr o Al a 250 Leu SIle Leu Asp Val 330 Thr Val Leu SIle Gy 155 I le Leu Asp Lys Leu 235 Ar g Al a Thr Gy Gy 315 Hi s Ser Lys G n Asn 140 Asn Lys Asp Al a Asn 220 Hi s Al a Asp Asp Val 300 Gy Hi s Thr Ser G n 380 Al a Pr o Ser Leu Asp 205 Val Hi s Sy Sy Al a 285 Leu Al a Val Val Asn 365 Val Cys Thr SIle Cys 190 Phe Thr Ar g Asp Thr 270 Lbt Asn l Ie Ar g Al a 350 Pr o Hi s Ar g Asp Thr 175 Al a Asp Val Asp Al a 255 Val G u Val Al a Ar g 335 Al a Val Leu Cys Leu 160 Val Al a Thr Thr Pr o 240 Tyr Asp Al a Thr Sy 320 Sy Lys Asn Asp <210> 415 <211> 1287 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1264) <223> FRXA00043 <400> 415 aacagcaggc ctcaagtccg aagataatta acctaaatcc gtagacataa gacatcatac Page 538 Gene Sequence 24- 2- 06. txt gtcctatgct tgctggaagg aagcaaataa cctcagaaag atg gca gaa gtg gtg lMt Ala Gu Val Val cat Hi s at t l Ie ggc Gy ttc Phe aat Asn gcg Al a ttg Leu gaa G u ct c Leu gat Asp 150 gcg Al a aat Asn t cc Ser gcc Al a aat Asn 230 ttg Leu caa G n acc Thr at c SIle ccc Pr o ggt Gy aat Asn agc Ser ctt Leu 120 ggt Gy at t SIle aaa Lys act Thr ggg Gy 200 gct Al a at g Net gct Al a aat Asn cac Hi s gaa G u ct c Leu aac Asn gcg Al a gtt Val ccc Pr o ttc Phe ccc Pr o tgg Tr p 170 ctt Leu act Thr gag G u ccg Pr o gca Al a 250 gca Al a ggg Gy ct c Leu ccc Pr o ggt Gy 75 cag G n tcg Ser tt g Leu at c SIle ggc Gy 155 at c SIle ct c Leu gat Asp aaa Lys ct g Leu 235 cgt Ar g ggt Gy gt g Val tct Ser acg Thr ggc Gy t at Tyr gcg Al a tgt Cys aac Asn 140 aac Asn aaa Lys gat Asp gca Al a aat Asn 220 cat Hi s gcc Al a caa G n at t SIle gga Gy 45 at t SIle gcg Al a cac Hi s ccg Pr o gaa G u 125 gca Al a cca Pr o tcg Ser ct c Leu gat Asp 205 gt g Val cat Hi s ggg Gy gca Al a gat Asp gaa G u gtt Val ttt Phe cgc Ar g gct Al a 110 gag G u tgc Cys aca Thr at c SIle tgc Cys 190 ttt Phe acg Thr agg Ar g gac Asp gtt Val 15 ggc Gy cca Pr o ccc Pr o cct Pr o gaa Gu 95 gac Asp ggc Gy cgt Ar g gat Asp aca Thr 175 gca Al a gat Asp gt c Val gat Asp gca Al a 255 aaa Lys ttt Phe gca Al a agt Ser acg Thr cat Hi s gca Al a ct g Leu tgt Cys ctt Leu 160 gt a Val gcg Al a acc Thr acg Thr ccc Pr o 240 t at Tyr aaa Lys ct c Leu cct Pr o ttt Phe gga Gy ggc Gy ct g Leu ct g Leu ggt Gy 145 gcc Al a gcg Al a cac Hi s act Thr gct Al a 225 ggc Gy gtt Val at t SIle caa G n aaa Lys at t SIle acg Thr acg Thr gca Al a tgc Cys 130 gct Al a cag G n ccg Pr o cac Hi s acc Thr 210 acg Thr agc Ser gag G u gaa G u ct c Leu aac Asn gat Asp cag G n acc Thr gcg Al a 115 ggc Gy caa G n gt g Val gaa G u at c SIle 195 agc Ser cat Hi s gt g Val ttg Leu gga Sy gaa G u gca Al a ctt Leu gac Asp gtg Val 100 cag G n att SIe aac Asn at c SIe act Thr 180 att SIe gca Al a ttg Leu ggc Gy at c SIe 260 Page 539 Gene Sequence 24- 2- 06. txt gac ggc gtg cat ttg gcc gat gga acg gtc gat cta gct cgt tcc aac 931 Asp G y Val His Leu Al a Asp G y Thr Val Asp Leu Ala Arg Ser Asn 265 270 275 aac gcc ttt ttc atc acg gac gcc atg gaa gcc gcc gga atg cca gac 979 Asn Al a Phe Phe I e Thr Asp Al a ebt G u Al a Al a G y Iet Pro Asp 280 285 290 ggt gag tac att ttg ggc gtt ttg aac gtc acc gtc acc gat ggc gtc 1027 Gy G u Tyr Ile Leu G y Val Leu Asn Val Thr Val Thr Asp y Val 295 300 305 gcc cgt ctg cgc gat ggc ggc gcc atc gcc ggg ggt acc agc aca cta 1075 Ala Arg Leu Arg Asp Gy y Ala lie Ala Gy y Thr Ser Thr Leu 310 315 320 325 gcg agt cag ttc gtg cac cac gtg cgc agg ggt atg acg ctt atc gac 1123 Ala Ser G n Phe Val His His Val Arg Arg G y IVet Thr Leu lie Asp 330 335 340 gcg acc ctc cac acc tca acc gtc gcc gcc aaa att ctc gga ctt agc 1171 Ala Thr Leu His Thr Ser Thr Val Ala Ala Lys IIe Leu G y Leu Ser 345 350 355 gat cac gaa atc gtt aaa tcc aac cct gta aat ttt gtg gtc ttt gac 1219 Asp His G u Ile Val Lys Ser Asn Pro Val Asn Phe Val Val Phe Asp 360 365 370 tca aac ggc cag tta caa cag gtc cat tta gac cat caa gta att 1264 Ser Asn G y n Leu Gn n Val His Leu Asp His n Val lie 375 380 385 taaatacgag caaaactttc ctg 1287 <210> 416 <211> 388 <212> PRT <213> Corynebacter i um gl utam cum <400> 416 Met Ala G u Val Val His Tyr n G u Asn Ala G y G n Ala Val Lys 1 5 10 Lys I e G u G y Arg I I e Val Thr Pro His G y Val lie Asp G y Phe 25 Leu n Leu G u Asn G y lie lie Thr G u Leu Ser G y G u Pro Al a 40 Pro Lys Asn Ala G y Phe His Pro G u Leu Pro Thr lie Val Pro Ser 55 Phe IIe Asp Leu His Asn His G y G y Asn G y G y Ala Phe Pro Thr 70 75 G y Thr G n Asp G n Ala Arg Asn Ala Ala G n Tyr His Arg G u His 90 Sy Thr Thr Val Net Leu Ala Ser Net Val Ser Ala Pro Ala Asp Ala 100 105 110 Leu Al a Ala n Val G u Asn Leu Ile Pro Leu Cys G u G u y Leu 115 120 125 Leu Cys G y lie His Leu G u G y Pro Phe IIe Asn Al a Cys Arg Cys 130 135 140 Page 540 Gene Sequence 24-2-06.txt Gy 145 Al a Al a Hi s Thr Al a 225 Gy Val Leu Al a Val 305 Gy et SIle Phe Hi s 385 Ala G n Asn Pro Val G u SIle 195 Ser Hi s Val Leu Ar g 275 et Asp Ser Leu Gy 355 Val Val Hi s 165 Asp Al a SIle Phe Al a 245 Al a Asn Asp Val Leu 325 Asp Ser Asp Asp 150 Al a Asn Ser Al a Asn 230 Leu Asp Asn Gy Al a 310 Al a Al a Asp Ser Phe lie Phe Pro Gy Leu Phe Leu 215 Al a Leu Gy Al a G u 295 Ar g Ser Thr Hi s Asn 375 Lys Thr Gy 200 Al a vet Al a Val Phe 280 Tyr Leu G n Leu G u 360 Gy Gy G u 185 Hi s Lys Pr o Al a Hi s 265 Phe SIle Ar g Phe Hi s 345 I le G n Gy 155 I le Leu Asp Lys Leu 235 Ar g Al a Thr Gy Gy 315 Hi s Ser Lys G n Ser Leu Asp 205 Val Hi s Gy Gy Al a 285 Leu Al a Val Val Asn 365 Val Asn Pro Thr Asp Leu SIle Cys 190 Phe Thr Ar g Asp Thr 270 et Asn l Ie Ar g Al a 350 Pr o Hi s Thr 175 Al a Asp Val Asp Al a 255 Val G u Val Al a Ar g 335 Al a Val Leu 160 Val Al a Thr Thr Pr o 240 Tyr Asp Al a Thr Gy 320 Gy Lys Asn Asp <210> 417 <211> 1584 <212> DNA <213> Cor ynebact er i um gl ut ai cum <220> <221> CDS <222> (101)..(1561) <223> RXN01752 <400> 417 gaaatgacgt gaccatcgat accaataccc aattgaaaga tcttgacctg gtcagccaag ttggccgtca gatcgtggca gaacaacagg tggggaggtc atg atg gaa caa gat 115 eVt eVt G u n Asp 1 ctc agc tac cgt gaa att ctt ccc ctc aac gcg agt gag gag aag aaa 163 Leu Ser Tyr Arg u Ile Leu Pro Leu Asn Ala Ser G u G u Lys Lys Page 541 Gene Sequence 24-2-06.txt aag Lys ct a Leu at t SIle gca Al a aca Thr t cc Ser aag Lys gt g Val cat Hi s 150 at c SIle aag Lys gga Gy gac Asp cgc Ar g 230 aac Asn gt g Val gt c Val gca Al a tct Ser gt g Val ctt Leu ttg Leu at c SIle aaa Lys 120 gcg Al a ct g Leu gcg Al a ccg Pr o cac Hi s 200 cca Pr o gct Al a agg Ar g ttt Phe ttg Leu at t l Ie tcg Ser gt g Val gga Gy aga Ar g gt a Val t ac Tyr ct g Leu gt a Val gag G u 170 gcg Al a gcc Al a ccc Pr o aat Asn aat Asn 250 ttc Phe ggt Gy gcc Al a gca Al a ttt Phe t ca Ser at c SIle gat Asp gt g Val gca Al a 140 ct c Leu gcg Al a ccc Pr o ggg Gy caa G n 220 gt g Val ct a Leu ct g Leu t cc Ser at t l Ie ttt Phe 45 at c SIle gca Al a at g bet gaa G u ttg Leu 125 t cc Ser at g Net gga Gy cgc Ar g gaa G u 205 ct g Leu tgc Cys act Thr cca Pr o aac gaa Gu act Thr ttg Leu acg Thr ttt Phe gat Asp 110 gt g Val at g tet ttg Leu gt g Val acc Thr 190 at t SIle cgt Ar g gt c Val gcg Al a ggc Gy 270 cat ggg Gy aga Ar g at g et tgt Cys t cc Ser 95 gcg Al a ccc Pr o cac Hi s gag Gu gat Asp 175 aag Lys gt c Val cgc Ar g cag G n tgg Tr p 255 gt c Val tta Leu ggg Gy ct c Leu acg Thr aga Ar g ct g Leu gcc Al a gct Al a gaa G u 160 cag G n ccg Pr o acg Thr gt g Val t ca Ser 240 ttc Phe at g let gt g Val aaa Lys ttt Phe at g Net at c SIle ttc Phe ggc Gy 130 gat Asp gat Asp gca Al a gcc Al a ttt Phe 210 gt g Val ttg Leu at t SIle at g let cgc Ar g gt c Val gct Al a t ac Tyr cgc Ar g cct Pr o 115 gaa G u t ac Tyr ct g Leu acg Thr tgt Cys 195 gac Asp gca Al a tcg Ser gaa G u aac Asn 275 gat Asp gcc Al a cgg Ar g ct c Leu gcg Al a 100 gag G u cct Pr o ccc Pr o ccc Pr o at c SIe 180 aac Asn gcg Al a ttt Phe t at Tyr t at Tyr 260 gca Al a Asn Hi s ctg ctc acg ggt gtc ctg aaa Leu Leu Thr G y Val Leu Lys Page 542 Gene Sequence 24-2-06.txt 280 285 290 gat ctc ggc gcg tgg gat cct ttc aat gtc aca gaa aat gcc gac ctc 1027 Asp Leu y Ala Trp Asp Pro Phe Asn Val Thr G u Asn Ala Asp Leu 295 300 305 ggc gta ccc atc gcg gca aaa gga tat tcc acc gcg gtg ttg gat tcg 1075 Sy Val Pro Ile Ala Al a Lys y Tyr Ser Thr Ala Val Leu Asp Ser 310 315 320 325 gtg acg tgg gag gaa gca aac tcc gac acc atc aac tgg ttg cgc cag 1123 Val Thr Trp G u u Al a Asn Ser Asp Thr Ile Asn Trp Leu Arg G n 330 335 340 cgt tct cgc tgg tac aag ggc tat ctg caa aca tgg ctt gtg tat atg 1171 Arg Ser Arg Trp Tyr Lys y Tyr Leu G n Thr Trp Leu Val Tyr IVet 345 350 355 cgc agg cca aag tgg tta gtc caa gag ctt ggc atc att cct gct gtg 1219 Arg Arg Pro Lys Trp Leu Val n u Leu y Ile Ile Pro Ala Val 360 365 370 cgt ttt acc ttc ctc atg gca ggc acc ccg atc att gcg gtg ctc aat 1267 Arg Phe Thr Phe Leu IVet Ala y Thr Pro Ile Ile Ala Val Leu Asn 375 380 385 ctg ctc ttt tgg tac ttg tcg ctc acg tgg att ctg ggc cag ccc ggc 1315 Leu Leu Phe Trp Tyr Leu Ser Leu Thr Trp lie Leu Gy n Pro y 390 395 400 405 acc att gag cag atg ttc cca cct gcg gtg tac tac cca gcg ttg gtg 1363 Thr Ile G u G n Iet Phe Pro Pro Ala Val Tyr Tyr Pro Ala Leu Val 410 415 420 tgt ttg gtg gtg gcc aat gct gcg acc atc ttt atg aat ctc att ggc 1411 Cys Leu Val Val Al a Asn Al a Al a Thr Ile Phe IVet Asn Leu I e G y 425 430 435 tgc cgg gaa ggc cgc gac ccc ttg ctg ctc atc gcg gtt ctc acg ttc 1459 Cys Arg G u y Arg Asp Pro Leu Leu Leu lie Ala Val Leu Thr Phe 440 445 450 ccg ctg tat tgg ctg ctc atg agc att gca gcg ttg aaa ggc acg tgg 1507 Pro Leu Tyr Trp Leu Leu IVet Ser lie Ala Ala Leu Lys y Thr Trp 455 460 465 caa ttg atc acg cga cca tcc tat tgg gag aaa act gcc cac gga ttg 1555 G n Leu IIe Thr Arg Pro Ser Tyr Trp G u Lys Thr Ala His G y Leu 470 475 480 485 gag gcg taagcggtgc ccatcgtcaa acc 1584 G u Al a <210> 418 <211> 487 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 418 IVet IVet u n Asp Leu Ser Tyr Arg u lie Leu Pro Leu Asn Ala 1 5 10 Ser G u G u Lys Lys Lys Ala Ala Leu I e Asp Ala lie u y Leu 25 Arg Val Arg Asp Pro Leu Leu Ser Ala Ser lie Ala Phe Thr Arg y Page 543 Gene Sequence 24-2-06.txt G n I e Phe Gy Al a Tyr Phe 145 Asp Val Lys SIle Val 225 Ar g Thr Ar g Thr G u 305 Al a Asn Tr p SIle SIle 385 Val Al a Tyr Ar g Pr o 115 G u Tyr Leu Thr Cys 195 Asp Al a Ser G u Asn 275 Val Al a Leu Leu Val 355 Pr o Val Phe G n SIle G u Asp G u Lys Thr 165 I le Tyr G u G u Ar g 245 Asp Pr o Lys Leu Ser 325 G n et Val Asn SIle Al a 70 Thr Ser Lys Val Hi s 150 I le Lys Gy Asp Ar g 230 Asn Val Val Asp Gy 310 Val Ar g Ar g Ar g Leu 390 Al a Al a Leu SIle Leu SIle 135 Leu Al a Val Leu vet 215 Ser Al a Tr p Pr o Leu 295 Val Thr Ser Ar g Phe 375 Leu Val Leu Leu SIle Lys 120 Al a Leu Al a Pr o Hi s 200 Pr o Al a Ar g Phe Leu 280 Gy Pr o Tr p Ar g Pr o 360 Thr Phe Ile Leu IVet Leu Val Val G y Phe I le G y Leu Ser 75 Asp Arg Phe Ile 90 G n Val Ser Asp 105 Thr Tyr Thr Val G n Leu Leu Ala 140 G n Val Leu Leu 155 Ala G u Ala Ala 170 Pro Ala G n Pro 185 Phe Al a Thr G y Asp Pro Leu G n 220 Ser Asn Thr Val 235 G n Asn Leu Leu 250 Asn Phe Leu Leu 265 G y G y Thr Ser Ala Trp Asp Pro 300 Ile Ala Ala Lys 315 Su u Ala Asn 330 Trp Tyr Lys y 345 Lys Trp Leu Val Phe Leu IVet Al a 380 Trp Tyr Leu Ser 395 Al a bet G u Leu 125 Ser bet Sy Ar g G u 205 Leu Cys Thr Pr o Asn 285 Phe Sy Ser Tyr G n 365 Sy Leu Cys Ser Al a Pr o Hi s G u Asp 175 Lys Val Ar g G n Tr p 255 Val Leu Val Ser Thr 335 G n Leu Pr o Tr p Thr Ar g Leu Al a Al a G u 160 G n Pr o Thr Val Ser 240 Phe vet Leu Thr Thr 320 I e Thr Sy SIe SIe 400 Page 544 Le u Tyr vb t Al a Le u 465 Thr O31n Al a Le u 435 Le u G31y Hi s G31y 405 ValI G31y Ph e Tr p Le u 485 I Ie Le u Ar g Le u 455 Le u Al a ene Sequence 24- 2- 06. txt O31u On I'bt Phe Pro Pro Ala Val Tyr 410 415 Val Val Al a As n Al a Al a Thr I Ie Phe 425 430 O31u G31y Ar g Asp Pr o Leu Leu Leu Ilie 440 445 Tyr Tr p Leu Leu I'b t Ser Ilie Ala Ala 460 Ilie Thr Ar g Pr o Ser Tyr Tr p O31u Lys 475 480 <210O> 419 <21 1> 689 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1 9) .(666) <223> FRXA01839 <400> 419 t tcct gct gc caggt gt c at g cgc at g aac gca I'b t Ar g I'b t As n Al a 1 5 cct gt c cca tt g Pr o Val Pr o Leu ac c Thr g at As p gca Al a gca Al a aag Ly s t ta Le u at g vb t t tg Le u tt c Ph e aac As n tt c Ph e gga O31y t cc Ser t at Tyr c aa O31n G31y 110 ct c Le u cct Pr o ct g Le u gt c ValI t cc Ser ac c Thr c aa O31n ct t Le u ccg Pr o t gg Tr p gt g ValI ac g Thr g aa O31u gcg Al a aac As n t gg Tr p at c I Ie at t I Ie ct g Le u 130 t ac Tyr ggt O31y g at As p 35 gt g ValI t gg Tr p ct t Le u at t I Ie gcg Al a 115 ggc O31y cca Pr o gt c ValI gcg Al a tt g Le u tt g Le u gt g ValI cct Pr o 1 00 gt g ValI c ag O31n gcg Al a aaa Ly s ct c Le u t cg Ser c ag O31n 70 at g vb t gt g ValI aat As n ggc (31y ct c Le u gt a ValI ac g Thr t ct Ser ag g Ar g tt t Ph e ct c Le u 120 at t I Ie ggc ggt (31y (1y gcg t gg Al a Tr p at c gcg I e Al a gag gaa (31u (1u t gg t ac Tr p Ty r aag t gg Lys Tr p t tc ct c Phe Leu t gg t ac Tr p Ty r cag at g (31n db t t tg gt g t gt t tg gt g gt g gcc Leu Val Cys Leu Val Val Al a Page 545 140 145 aat gct gcg acc atc ttt atg aat c Asn Ala Ala Thr Ile Phe IVet Asn L 160 gac ccc ttg ctg ctc atc gcg gtt c Asp Pro Leu Leu Leu I e Ala Val L 175 1 ctc atg agc att gca gcg ttg aaa g Leu IVet Ser lie Ala Ala Leu Lys C 190 195 cca tcc tat tgg gag aaa act gcc c Pro Ser Tyr Trp G u Lys Thr Ala H 205 210 ccatcgtcaa acc <210> 420 <211> 216 <212> PRT <213> Cor ynebact eri um gl ut am cum Sequence 24-2-06.txt 150 155 tc att ggc tgc cgg gaa ggc cgc eu lie y Cys Arg u Gy Arg 165 170 t c acg ttc ccg ctg tat tgg ctg .eu Thr Phe Pro Leu Tyr Trp Leu 80 185 igc acg tgg caa ttg atc acg cga 3y Thr Trp G n Leu Ile Thr Arg 200 ac gga ttg gag gcg taagcggtgc is G y Leu G u Al a 215 <400> 420 Mvt Arg MVet 1 Leu Thr G y Thr G u Asp Thr Al a Val lie Asn Trp Thr Trp Leu S y lie l ie Ile Il e Al a 115 l e Leu G y 130 Tyr Tyr Pro 145 Phe IVet Asn I e Al a Val Ala Leu Lys 195 Lys Thr Al a 210 Asn Ala Pro 5 Val Leu Lys Al a Asp Leu Leu Asp Ser Leu Arg G n 70 Val Tyr IVet Pro Al a Val 100 Val Leu Asn G n Pro G y Ala Leu Val 150 Leu Ile G y 165 Leu Thr Phe 180 G y Thr Trp Hi s G y Leu Val Asp Gy Val 55 Ar g Ar g Ar g Leu Thr 135 Cys Cys Pr o G n G u 215 Leu Sy Ar g Tr p Ar g Pr o Thr 105 Phe G u Val G u Tyr 185 I le Sy 10 Al a SIle G u Tr p Lys 90 Phe Tr p G n Val Sy 170 Tr p Thr Sy Tr p Al a G u Tyr Tr p Leu Tyr bet Al a 155 Ar g Leu Ar g Thr Asp Al a Al a Lys Leu bet Leu Phe 140 Asn Asp Leu Pr o Ser Pr o Lys Asn Sy Val Al a Ser 125 Pr o Al a Pr o Met Ser 205 Hi s Asn Tyr Asp Leu G u Thr Thr Al a Thr Leu 175 I e Tr p Page 546 ene Sequence 24- 2- 06. txt <210O> 421 <21 1> 1050 <212> DNA <213> COr ynebact er i urn gi ut arr cumn <220> <221 ODS <222> (1027) <223> RXA01859 <400> 421 t acgcccagg gt t tccat at t ggt aaat ct aagccgatt g ct cgagacga accaaaccgc t acct ggggg t aggaagaat at gaat t tat agct act tat atg aaa aag aag agc 11! l'bt Lys Lys Lys Ser tt t Ph e at g vb t t ca Ser tt t Ph e gt t ValI ac c Th r ccg Pr o ac c Th r gga G31y gt t ValI 1 50 gct A a gcc A a at c I Ie at a I Ie ggt G31y g ac As p t ta Le u cgc Ar g at t I Ie aac As n 120 at t I Ie gt t ValI gga G31y gga (31y ag a Ar g t tg Le u aaa Ly s at t I Ie tt c Ph e cga Ar g at g vb t ct c Le u gca A a g at As p gag (31u 1 70 aat As n gt c ValI ct a Le u gaa (31u cca Pr o gt c ValI 75 ag c Ser gca A a gt g ValI t t g Le u aat As n 155 gt c ValI cag (31n at c I Ie t gg Tr p ac c Th r cca Pr o tt t Ph e t cc Ser g aa G31u cca Pr a aaa Ly s 140 t gc cys tt g Le u gct A a ggt G31y ct t Le u g aa G31u 45 gt c ValI at c I Ie gt a ValI ac c Th r gca A a 125 t ca Ser act Th r g aa G31u tt g Le u at c I Ie gca A a gt c ValI ag g Ar g gct A a g ac As p cga Ar g 110 c ac Hi s c aa O31n g at As p ac a Th r ag c Ser 1 90 ggc G31y 15 gt t ValI tt t Ph e gt a ValI gcc A a ggc G31y 95 ggg G31y aat As n cat Hi s g aa O31u gt c ValI 1 75 cgg Ar g gt c ValI ac c Th r g at As p tt g Le u ac a Th r 80 g aa O31u gt a ValI gag O31u gag O31u act Th r 1 60 aat As n at g vb t ggc G31y t ct Ser t gg Tr p gct A a g aa O31u gt g ValI cat Hi s g aa O31u 130 cca Pr a g aa O31u aag Ly s ccc Pr a at c I Ie g at As p aaa Ly s gcg A a cga Ar g cca Pr a gga G31y 115 ag a Ar g g aa O31u tt a Le u tt t Ph e ac a Th r 1 gcc A a 20 cca Pr a gt g ValI at t I Ie ac c Th r tt a Le u 1 00 ccg Pr a at t I Ie cgc Ar g gcc A a aag Ly s 1 tt g Le u ggg G31y gca A a ct c Le u at c I Ie gt a ValI gcg A a at t I Ie act Th r at c I Ie cgt Ar g 1 aag Ly s ggg G31y gag aat gac at t gt g at g at c gt t gac gct gat aca gca ct t gat caa Page 547 Su Asn Asp lie Val IVet lie 200 ggt ttc ctc aag gaa gca cgg Gy Phe Leu Lys G u Al a Arg 215 220 atg gcc gtg ggc gga ttg ttc lMt Al a Val G y G y Leu Phe 230 235 ggc caa tat cag cgc aac gaa Gy On Tyr On Arg Asn u 250 cga cgc cgc gga cgt gtg ttt Arg Arg Arg y Arg Val Phe 265 cgg cca cgc ggc ctg cgg aca Arg Pro Arg y Leu Arg Thr 280 ccc gga cgt aaa gcc gat gtt Pro y Arg Lys Ala Asp Val 295 300 taatgagttg accctggctt tga <210> 422 <211> 309 <212> PRT <213> Cor ynebact er i um gl ut ar Gene Sequence 24-2-06.txt Val Asp Ala Asp Thr Ala Leu 205 210 cgc cgc ttt gag tct gat cgc Arg Arg Phe G u Ser Asp Arg 225 tac ggt gag tca ggc tcc gga Tyr G y u Ser G y Ser G y 240 tac acc cgt tat agc cgt gac Tyr Thr Arg Tyr Ser Arg Asp 255 gtt ttg act gga aca gcg tcg Val Leu Thr y Thr Ala Ser 270 275 gta gcg gaa tca cgc ggg aca Val Ala u Ser Arg y Thr 285 290 tat gac acc gcg ggc gtt gac Tyr Asp Thr Ala y Val Asp 305 Asp gct Al a tgg Tr p at c SIe 260 gct Al a ttg Leu cga Ar g 787 835 883 931 979 1027 1050 <400> 422 let Lys L 1 Leu G y I Leu Ser A Arg Trp L Val Ala A Val u A Arg Val P Phe Hi s G 1 Al a u A 130 Pro Pro G 145 ys Lys le Al a sp Pro ys Val la Ile rg Thr ro Leu 100 ly Pro 15 rg Ile Iu Arg Ser 5 Gy Al a Leu SIle Val Al a SIle Thr SIle Ar g 165 Phe Pro lie Ala Gy Pr o Asp Al a Asn Lys SIle Thr 135 Val Leu 25 Al a Tyr l Ie Tyr Val 105 Val G n Al a Val Leu G u Pr o Val Ser Al a Val Leu Asn 155 Gy Leu G u Val SIle Val Thr Al a 125 Ser Thr SIle Al a Val Ar g Al a Asp Ar g 110 Hi s G n Asp Sy Val Phe Val Al a Sy Sy Asn Hi s G u Thr G u Leu Al a Al a Gu Gy Val u Val 170 Leu G u Thr Val Asn 175 Page 548 Gene Sequence 24 Asn Lys Phe Lys Lys Al a G y G y Leu Asn G n 180 185 Leu Pro Thr Leu Gy G u Asn Asp lie Val IVet 195 200 Thr Al a Leu Asp n G y Phe Leu Lys G u Al a 210 215 Ser Asp Arg Ala Leu IVet Ala Val y y Leu 225 230 235 G y Ser G y Trp Leu G y n Tyr n Arg Asn 245 250 Ser Arg Asp lie Tyr Arg Arg Arg y Arg Val 260 265 Thr Ala Ser Ala Phe Arg Pro Arg y Leu Arg 275 280 Arg y Thr Leu lie Pro y Arg Lys Ala Asp 290 295 G y Val Asp Arg Arg 305 <210> 423 <211> 882 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(859) <223> RXA00042 <400> 423 gtggtctttg actcaaacgg ccagttacaa caggtccatt atacgagcaa aactttcctg ataataaaag gagtccgacc -2-06. txt Ala Leu Ser 190 IIe Val Asp 205 Arg Arg Arg 220 Phe Tyr G y G u Tyr Thr Phe Val Leu 270 Thr Val Al a 285 Val Tyr Asp 300 Ar g Al a Phe G u Ar g 255 Thr G u Thr tgc Cys ccc Pr o cct Pr o gt g Val tta Leu ttc Phe gac Asp gca Al a agc Ser ttc Phe cgc Ar g gac Asp caa G n aag Lys t ac Tyr aac Asn gat Asp at c SIle gaa G u ggc Sy caa G n tgc Cys gaa G u 75 gac Asp gt c Val gga Sy gag G u aag Lys aac Asn at c SIle ggc Sy acc Thr ct c Leu 45 gca Al a agc Ser gtt Val aaa Lys ttg Leu at t SIle ttc Phe t ac Tyr gat Asp gca gcg Al a Al a 15 ggg ctt G y Leu cgc atg Arg IVet ttg ttg Leu Leu ttc aaa Phe Lys 80 gaa gag Page 54u Page 549 t agaccat ca agt aat t t aa atg gac atc atc atc IVet Asp Ile Ile Il e 1 gca gcc ctg atc gca Ala Ala Leu Ile Ala gca act gga tcg tca Al a Thr G y Ser Ser tat gaa gct ggg gaa Tyr G u Al a y G u gat gaa tac gtg gga Asp G u Tyr Val y acc att cgt aaa gag Thr lie Arg Lys u gtc tac agc cca gat Val Tyr Ser Pro Asp Gene Sequence 24-2-06.txt ggt Gy at c l Ie ggc Gy aag Lys 150 ttc Phe ggc Gy gga Gy at g Net gtt Val 230 ct c Leu aac Asn gca Al a 120 at c SIle cag G n acc Thr ttg Leu gcc Al a 200 cca Pr o gaa ggu gag Gu cct gat Pro Asp 105 gaa t cc Su Ser gct ttc Al a Phe gcg ctg Al a Leu atc gaa Ile G u 170 t cc cgc Ser Arg 185 gac gcc Asp Al a ggt tcc G y Ser gca gca Al a Al a caa tta G n Leu 250 cca Pr o gtt Val aat Asn cac Hi s 155 gag G u gcg Al a at c l Ie at c SIle gca Al a 235 t ac Tyr gat Asp gag G u 140 cct Pr o gt c Val caa G n cgc Ar g ct g Leu 220 t cc Ser gaa G u gtt Val 125 cca Pr o aaa Lys cca Pr o aac Asn gga Gy 205 cag G n aag Lys gca Al a 110 caa G n t ca Ser act Thr acc Thr at c SIle 190 act Thr at g Met ct g Leu gct Al a at c SIle tct Ser gt g Val cac Hi s 175 gt g Val gt g Val cac Hi s gaa G u gca Al a ctt Leu tct Ser gag G u 160 gcc Al a ttg Leu gaa G u aac Asn aac Asn 240 g tat gag gca u Tyr G u Ala 115 c atc ggc gga y lie y G y 130 g tca gga ctg u Ser G y Leu c aac gct cga p Asn Ala Arg c acc cag ggt u Thr n G y 180 g gca act ggt I Ala Thr y 195 c cca ctg acc y Pro Leu Thr 210 t gcc acc atc n Ala Thr Ile t gat cac tac a Asp His Tyr aag ctg cgc Lys Leu Arg tagaaacaaa aaggaaagta gtg <210> 424 <211> 253 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 424 IVet Asp Ile Ile lie Cys Lys Asp u 1 5 Ala Ala Leu lie Ala Pro Phe Ala Thr Ala Thr Gy Ser Ser Pro Leu Ser Thr 40 Tyr G u Ala Gy G u Val Ser Phe Lys Asp G u Tyr Val y Leu Thr Arg Asp 70 Thr lie Arg Lys G u Phe Thr Asp His Val Tyr Ser Pro Asp G y Ala Asn Pro G n u 10 Lys G y Tyr G n Asn Cys I Asp Gu Ile Asp I 90 Asp Pro Page 550 Gy Thr Leu Al a Ser Val G u Al a Gy Ar g Leu Phe G u Al a G u Gy Leu 145 Asp Leu Val Sy Asn 225 Al a G u 115 Gy Sy Al a G n Thr 195 Leu Thr Hi s Lys Asn Thr Phe 165 Leu G u Al a SIle Ar g 245 Al a Hi s 135 Val Asn Thr Lys Cys 215 Asp Met Gene Sequence 24-2-06. t xt 105 110 Ala u Ser Val Asp Val G n 120 125 Ile Ala Phe Asn G u Pro Ser 140 G n Ala Leu His Pro Lys Thr 155 Thr Ile G u G u Val Pro Thr 170 Leu Ser Arg Ala n Asn Ile 185 190 Ala Asp Ala lie Arg Gy Thr 200 205 Pro y Ser Ile Leu n IVet 220 G u Ala Ala Ala Ser Lys Leu 235 G u G n Leu Lys Leu Arg 250 SIe Ser Val Hi s 175 Val Val Hi s G u <210> 425 <211> 1998 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1975) <223> RXA01482 <400> 425 ttgcgtgctg caact t aatt atggtcctcc cagctcagt g ctcgtccatt aagtgatcga gaaaaagttg ttgtaaagt c at t SIle ct a Leu gac Asp aag Lys cct Pr o cat gga G y gt a Val gca Al a gcc Al a cca Pr o ggc at t I e gtt Val at t l Ie aag Lys tct Ser acc ggc G y gaa G u gct Al a gtt Val at t SIle 75 gat caa G n gga G y at t l Ie gct Al a ttg Leu gt c gcg Al a ct a Leu cac Hi s 45 gca Al a gga Gcy aac ggc G y cgt Ar g gcc Al a ct a Leu at t SIle gct gac t cc Asp Ser 15 cgc ctg Arg Leu aat ggt Asn y gat gca Asp Al a gga cac G y Hi s 80 cac ccc Page 551 tgctgtgtgg attgtttatt atg cgc atg tgt gga IVet Arg IVet Cys y 1 cgt gat tac ttt gct Arg Asp Tyr Phe Ala gaa tac cgc gga tat G u Tyr Arg y Tyr gag att agc tac cga G u lie Ser Tyr Arg gaa atc gct aaa gca G u Ile Ala Lys Ala acc cgt tgg gca act Thr Arg Trp Ala Thr cac gtt gtt tcc aat His Gy Gy Pro Thr Asp Val Gene Sequence 24- 2- 06. txt Asn Ala His Pro His Val Val Ser Asn 95 100 ggc G y ct g Leu gat Asp gca Al a 150 gag G u at c SIle ggc G y cgc Ar g gat Asp 230 cct Pr o ggt Sy gat Asp gag G u at t SIle 310 gcc Al a gag G u ctt Leu tct Ser 120 gaa G u ggt Gy gct Al a gca Al a aac Asn 200 gct Al a t ac Tyr gac Asp ttc Phe ct g Leu 280 cgc Ar g gct Al a gag G u cgt Ar g gt a Val ctt Leu gct Al a ct c Leu acc Thr 170 cgt Ar g ct c Leu gag G u at c SIle gag G u 250 gag G u ggc Gy gat Asp ggt Gy tgg Tr p 330 cgc Ar g gt a Val t cc Ser gct Al a acc Thr 155 ct g Leu cgt Ar g gga G y ct g Leu acc Thr 235 tgg Tr p aag Lys cgt Ar g gaa G u act Thr 315 tgc Cys gac Asp cac Hi s gct Al a tct Ser 140 ctt Leu ct a Leu aac Asn tct Ser gct Al a 220 aac Asn gac Asp gaa G u ctt Leu gct Al a 300 gca Al a cgc Ar g cca Pr o aac Asn aag Lys 125 ttg Leu gct Al a gct Al a tct Ser gac Asp 205 aat Asn ttt Phe gct Al a at c SIle gat Asp 285 at t l Ie gct Al a at c SIle at c SIle ggc G y 110 ggc Gy ctt Leu at g Net at t l Ie cct Pr o 190 gtt Val gac Asp gat Asp gca Al a cac Hi s 270 gaa G u ct g Leu t at Tyr cca Pr o ct c Leu 350 at c SIle t ac Tyr gct Al a cag G n cat Hi s 175 ttg Leu tct Ser cag G n gga G y gct Al a 255 gat Asp gat Asp cgt Ar g gca Al a acc Thr 335 aac Asn at c SIle aac Asn gaa G u ct g Leu 160 gct Al a gtt Val ggc G y gtt Val t ca Ser 240 gct Al a cag G n ggc Gy agt Ser ggc Gy 320 gag ggu gag G u gaa G u ttt Phe at t SIle 145 acc Thr gat Asp at c SIle ttt Phe gtt Val 225 gaa G u gaa G u cca Pr o aag Lys gt c Val 305 cag G n gt g Val aag Lys aac Asn gt a Val 130 t ac Tyr ggt Gy cac Hi s ggc Gy at t SIle 210 acc Thr gca Al a aag Lys gca Al a ct c Leu 290 gac Asp gtt Val gag G u acc Thr gcg Al a gat Asp act Thr cgc Ar g gac Asp 180 ggc Gy t ac Tyr acc Thr ggc G y ggc G y 260 gtt Val ctt Leu at c SIe cgt Ar g gct Al a 340 gtt Val 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 gca ttg tcc cag tcc ggc gag acc atg gat acc ctc atg gct gtt cgc Page 552 Al a Leu Ser G n Ser G y G u 360 Gene Sequence 24-2-06.txt Thr IVet Asp Thr Leu IVet Ala Val Arg 365 370 cac Hi s gga G y 390 ggc G y act Thr aag Lys cct Pr o ctt Leu 470 cac Hi s at c SIle ggc G y cct Pr o at t SIle 550 gaa G u cct Pr o cag G n cag G n cgt Ar g act Thr gag G u tct Ser gct Al a 440 aag Lys caa G n ggt Sy t ac Tyr at t SIle 520 cct Pr o gag G u gat Asp gcc Al a ttt Phe 600 cgt Ar g cag G n cca Pr o gct Al a 410 ttg Leu gag G u cag G n at g et cca Pr o 490 cac Hi s ttg Leu ggt Sy cgt Ar g gct Al a 570 acc Thr tgc Cys ct g Leu ggt G y cgt Ar g 395 gt g Val ctt Leu gtt Val cag G n gca Al a 475 gtt Val gct Al a gtt Val cgc Ar g gca Al a 555 gt c Val ct g Leu gct Al a gca Al a gcc Al a 380 gaa G u gcg Al a ggc G y tct Ser gt c Val 460 gat Asp gcg Al a gaa G u gag G u gat Asp 540 cgt Ar g aac Asn at g let gt g Val aag Lys 620 aag Lys gca Al a t cc Ser ct g Leu t cc Ser 445 at c SIle gct Al a ctt Leu ggt G y gaa G u 525 t cc Ser ggc G y gat Asp cag G n gca Al a 605 tct Ser gtt Val gat Asp acc Thr t ac Tyr 430 at t SIle gat Asp aag Lys gag G u ttc Phe 510 ggc G y ct g Leu gct Al a t ac Tyr cct Pr o 590 acc Thr gt c Val gtt Val gcg Al a aag Lys 415 ttg Leu ct g Leu gca Al a tct Ser ggt G y 495 gct Al a cag G n cac Hi s gt c Val gcc Al a 575 ct g Leu gca Al a acc Thr gct Al a t cc Ser 400 gcg Al a gct Al a gac Asp gaa G u gt g Val 480 gcg Al a gca Al a ccg Pr o t cc Ser acc Thr 560 aac Asn ct g Leu aag Lys gt c Val at t l Ie 385 ct g Leu ttc Phe cag G n agc Ser gag G u 465 ct g Leu ttg Leu ggc G y at c SIle aag Lys 545 at c SIle ttc Phe t cc Ser ggc G y gaa 625 tgt Cys t ac Tyr ttg Leu ct g Leu ct g Leu 450 cag G n ttc Phe aag Lys gag G u ttc Phe 530 gtt Val gt g Val at c SIle acc Thr t ac Tyr 610 act Thr t ac Tyr cag G n 420 ggc G y gag G u aag Lys ggc G y aag Lys 500 aag Lys at c SIle t cc Ser gca Al a cgc Ar g 580 cct Pr o gt g Val gtt Val gct Al a 405 at c SIe aac Asn at g let aag Lys cgc Ar g 485 gag G u cac Hi s gt g Val aac Asn gag G u 565 at t SIe ct g Leu gat Asp 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1985 1998 t aaaaagat t tcgcttctcg acg Page 553 Gene Sequence 24-2-06.txt <210> 426 <211> 625 <212> PRT <213> Corynebact eri um gl ut arri cum <400> 426 IVet Arg Iet Cys Gy Ile Val Gy Tyr Ile Gy Gn Ala Gy Asp Ser 1 5 10 Arg Asp Tyr Phe Ala Leu Asp Val Val Val G u y Leu Arg Arg Leu 25 G u Tyr Arg G y Tyr Asp Ser Al a y I I e Ala IIe His Ala Asn G y 40 G u lie Ser Tyr Arg Lys Lys Ala y Lys Val Ala Ala Leu Asp Ala 55 G u I I e Ala Lys Ala Pro Leu Pro Asp Ser lie Leu y lie y His 70 75 Thr Arg Trp Ala Thr His G y G y Pro Thr Asp Val Asn Ala His Pro 90 His Val Val Ser Asn G y Lys Leu Ala Val Val His Asn y I I e I I e 100 105 110 G u Asn Phe Ala G u Leu Arg Ser G u Leu Ser Ala Lys y Tyr Asn 115 120 125 Phe Val Ser Asp Thr Asp Thr G u Val Ala Ala Ser Leu Leu Ala G u 130 135 140 I le Tyr Asn Thr G n Al a Asn G y Asp Leu Thr Leu Al a Iet G n Leu 145 150 155 160 Thr G y G n Arg Leu G u G y Ala Phe Thr Leu Leu Ala lie His Ala 165 170 175 Asp His Asp Asp Arg I e Val Ala Ala Arg Arg Asn Ser Pro Leu Val 180 185 190 I e Gy Val Gy Gu Gy Gu Asn Phe Leu Gy Ser Asp Val Ser Gy 195 200 205 Phe Ile Asp Tyr Thr Arg Lys Ala Val G u Leu Ala Asn Asp G n Val 210 215 220 Val Thr lie Thr Ala Asp Asp Tyr Ala lie Thr Asn Phe Asp y Ser 225 230 235 240 G u Ala Val y Lys Pro Phe Asp Val G u Trp Asp Ala Ala Ala Ala 245 250 255 G u Lys G y G y Phe G y Ser Phe et G u Lys G u IIe His Asp G n 260 265 270 Pro Al a Ala Val Arg Asp Thr Leu IVet y Arg Leu Asp G u Asp y 275 280 285 Lys Leu Val Leu Asp G u Leu Arg lie Asp G u Ala lie Leu Arg Ser 290 295 300 Val Asp Lys Ile Val I e Val Ala Cys y Thr Ala Ala Tyr Ala y 305 310 315 320 Page 554 Gene Sequence 24- 2- 06. txt Sn Val Ala Arg Tyr Ala lle u His Trp Cys Arg lie Pro Thr u 325 330 335 Val G u Leu Ala His G u Phe Arg Tyr Arg Asp Pro Ile Leu Asn G u 340 345 350 Lys Thr Leu Val Val Al a Leu Ser G n Ser G y G u Thr IVet Asp Thr 355 360 365 Leu et Ala Val Arg His Ala Arg G u G n y Ala Lys Val Val Ala 370 375 380 Ile Cys Asn Thr Val G y Ser Thr Leu Pro Arg G u Ala Asp Ala Ser 385 390 395 400 Leu Tyr Thr Tyr Ala G y Pro G u Ile Ala Val Ala Ser Thr Lys Ala 405 410 415 Phe Leu Ala n lie Thr Ala Ser Tyr Leu Leu y Leu Tyr Leu Ala 420 425 430 G n Leu Arg y Asn Lys Phe Ala Asp G u Val Ser Ser lie Leu Asp 435 440 445 Ser Leu Arg u IVet Pro u Lys Ile n G n Val Ile Asp Ala u 450 455 460 G u G n Ile Lys Lys Leu G y G n Asp IVet Al a Asp Al a Lys Ser Val 465 470 475 480 Leu Phe Leu G y Arg His Val G y Phe Pro Val Ala Leu G u G y Ala 485 490 495 Leu Lys Leu Lys G u I e Ala Tyr Leu His Ala G u G y Phe Ala Ala 500 505 510 G y G u Leu Lys Hi s y Pro IIe Ala Leu Val G u G u G y G n Pro 515 520 525 Ile Phe Val lie Val Pro Ser Pro Arg G y Arg Asp Ser Leu His Ser 530 535 540 Lys Val Val Ser Asn I e n G u Ile Arg Ala Arg Gy Ala Val Thr 545 550 555 560 Ile Val Ile Al a u G u y Asp G u Ala Val Asn Asp Tyr Al a Asn 565 570 575 Phe Ile Ile Arg Ile Pro G n Ala Pro Thr Leu Iet G n Pro Leu Leu 580 585 590 Ser Thr Val Pro Leu n lie Phe Ala Cys Ala Val Ala Thr Ala Lys 595 600 605 Sy Tyr Asn Val Asp G n Pro Arg Asn Leu Ala Lys Ser Val Thr Val 610 615 620 G u 625 <210> 427 <211> 666 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS Page 555 Gene Sequence 24-2-06.txt <222> <223> RXN03179 <400> 427 gct cgt gag gca tgg cgc att ttc atg tcc cac tgg gat ctc tac gca 48 Ala Arg G u Ala Trp Arg Ile Phe Iet Ser His Trp Asp Leu Tyr Ala 1 5 10 gga acc gca act ggc tac tgg gtg gag cag gaa ttt gag cac gtt ttc 96 G y Thr Ala Thr G y Tyr Trp Val G u G n G u Phe G u His Val Phe 25 ggc atc aac gcg gag cgc ctg aat gtt ggc acc cca gaa cat gct gac 144 G y IIe Asn Ala G u Arg Leu Asn Val G y Thr Pro G u His Ala Asp 40 gcc atc ttt gat gag ctg acc gat att ctt gcc aag cca gat ttc cga 192 Ala lie Phe Asp G u Leu Thr Asp Ile Leu Ala Lys Pro Asp Phe Arg 55 cca cgc gca ctg gct gag cag ttc aac ttg gaa gtt cta gcc acc acc 240 Pro Arg Ala Leu Ala G u G n Phe Asn Leu G u Val Leu Ala Thr Thr 70 75 gac gat ccg ctc gat gac ctg gca gat cac aag gca ctg gca gat gat 288 Asp Asp Pro Leu Asp Asp Leu Ala Asp His Lys Ala Leu Ala Asp Asp 90 cca acc ttc tcc cct cgt gtg ctc cct acc ttc cgc cca gac gca tac 336 Pro Thr Phe Ser Pro Arg Val Leu Pro Thr Phe Arg Pro Asp Ala Tyr 100 105 110 acc aag atg tac aac gct ggt tgg gca gaa aaa acc acc aag ctt atc 384 Thr Lys IVet Tyr Asn Al a G y Trp Al a G u Lys Thr Thr Lys Leu Ile 115 120 125 gat acc gca ggt gac ggc aag gca ggc tgg gag ggt tac ctt cag gca 432 Asp Thr Al a y Asp y Lys Ala y Trp u y Tyr Leu n Ala 130 135 140 atg cgc aac cgc cgc cag tac ttc atc aat cac ggt gca acc tcc gcg 480 IVet Arg Asn Arg Arg G n Tyr Phe I e Asn Hi s G y Al a Thr Ser Al a 145 150 155 160 gac cac ggt ctc cac gac acc gac acc acc cca ctg agc cac aaa gat 528 Asp His G y Leu His Asp Thr Asp Thr Thr Pro Leu Ser His Lys Asp 165 170 175 gcc cag aag atc ttg gac aag ggt ctc gct ggc aca gca acc ttg gct 576 Ala G n Lys Ile Leu Asp Lys y Leu Ala y Thr Ala Thr Leu Ala 180 185 190 gaa atg cat gcc ttc gaa gcc aac acc acc tac cgt tcg cgg aaa tgt 624 G u IVet His Ala Phe G u Ala Asn Thr Thr Tyr Arg Ser Arg Lys Cys 195 200 205 cca aga aga cgg ctg gtc atg acc atc cac cag gtg tgt acc 666 Pro Arg Arg Arg Leu Val IVet Thr Ile His G n Val Cys Thr 210 215 220 <210> 428 <211> 222 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 428 Ala Arg u Ala Trp Arg Ile Phe IVet Ser His Trp Asp Leu Tyr Ala Page 556 Gene Sequence 24-2-06.txt Sn G u Phe G u Gy Sy Al a Pr o Asp Pr o Thr Asp bet 145 Asp Al a G u Pr o Al a Asn Phe Al a Pr o Phe bet 115 Al a Asn Gy Lys Hi s 195 Ar g Gy G u G u Al a Asp Pr o Asn Asp Ar g Hi s 165 Leu Phe Leu Trp Val Leu Asn 40 Thr Asp G n Phe Leu Al a Val Leu Sy Trp 120 Lys Al a 135 Tyr Phe Thr Asp Lys G y Al a Asn 200 et Thr 215 G u Val SIle Asn Asp Pr o 105 Al a Gy I e Thr Leu 185 Thr SIle Gy Leu Leu Hi s 90 Thr G u Tr p Asn Thr 170 Al a Thr Hi s Thr Al a G u Lys Phe Lys G u Hi s 155 Pr o Sy Ty r G n Pr o Lys Val Al a Ar g Thr Sy 140 Sy Leu Thr Ar g Val 220 u Pr o Leu Leu Pr o Thr 125 Tyr Al a Ser Al a Ser 205 Cys Hi s Hi s Asp Al a Al a Asp 110 Lys Leu Thr Hi s Thr 190 Ar g Thr Val Al a Phe Thr Asp Al a Leu G n Ser Lys 175 Leu Lys <210> 429 <211> 672 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> <223> FRXA02872 <400> 429 gct cgt gag gca tgg cgc att ttc atg Al a Ar g G u Al a Tr p Arg lie Phe Iet 1 5 gga acc gca act ggc tac tgg gtg gag Sy Thr Ala Thr y Tyr Trp Val G u 25 ggc atc aac gcg gag cgc ctg aat gtt Gy lie Asn Ala Gu Arg Leu Asn Val 40 gcc atc ttt gat gag ctg acc gat att Ala lle Phe Asp G u Leu Thr Asp I e 55 tcc cac tgg gat ctc tac gca Ser His Trp Asp Leu Tyr Ala 10 cag gaa ttt gag cac gtt ttc G n u Phe G u His Val Phe ggc acc cca gaa cat gct gac Sy Thr Pro G u His Ala Asp ctt gcc aag cca gat ttc cga Leu Ala Lys Pro Asp Phe Arg Page 557 Gene Sequence 24-2-06.txt cca cgc gca ctg gct gag cag ttc aac Pro Arg Ala Leu Ala G u G n Phe Asn 70 gac gat ccg ctc gat gac ctg gca gat Asp Asp Pro Leu Asp Asp Leu Ala Asp cca acc ttc tcc cct cgt gtg ctc cct Pro Thr Phe Ser Pro Arg Val Leu Pro 100 105 acc aag atg tac aac gct ggt tgg gca Thr Lys IVet Tyr Asn Ala Gy Trp Al a 115 120 gat acc gca ggt gac ggc aag gca ggc Asp Thr Al a G y Asp G y Lys Ala G y 130 135 atg cgc aac cgc cgc cag tac ttc atc Iet Arg Asn Arg Arg G n Tyr Phe Ile 145 150 gac cac ggt ctc cac gac acc gac acc Asp Hi s G y Leu Hi s Asp Thr Asp Thr 165 gcc cag aag atc ttg gac aag ggt ctc Al a n Lys Ile Leu Asp Lys G y Leu 180 185 gaa atg cat gcc ttc gaa gcc aac acc G u IVet His Ala Phe G u Ala Asn Thr 195 200 tcc caa gaa gac ggc ctg gtc atg acc Ser n G u Asp y Leu Val IVet Thr 210 215 <210> 430 <211> 224 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 430 Ala Arg u Ala Trp Arg Ile Phe IVet 1 5 Sy Thr Ala Thr Gy Tyr Trp Val G u 25 Gy lie Asn Ala u Arg Leu Asn Val 40 Ala lie Phe Asp G u Leu Thr Asp Ile 55 Pro Arg Ala Leu Ala G u G n Phe Asn 70 Asp Asp Pro Leu Asp Asp Leu Ala Asp Pro Thr Phe Ser Pro Arg Val Leu Pro 100 105 ttg Leu cac Hi s 90 acc Thr gaa G u tgg Tr p aat Asn acc Thr 170 gct Al a acc Thr gaa gtt Su 75 aag Lys ttc Phe aaa Lys gag G u cac Hi s 155 cca Pr o ggc G y t ac Tyr val gca Al a cgc Ar g acc Thr ggt G y 140 ggt G y ct g Leu aca Thr cgt Ar g cta gcc acc acc Leu Ala Thr Thr ctg gca gat gat Leu Ala Asp Asp cca gac gca tac Pro Asp Al a Tyr 110 acc aag ctt atc Thr Lys Leu Ile 125 tac ctt cag gca Tyr Leu G n Ala gca acc tcc gcg Ala Thr Ser Ala 160 agc cac aaa gat Ser His Lys Asp 175 gca acc ttg gct Ala Thr Leu Ala 190 ttc gcg gaa atg Phe Ala G u IVet 205 atc cac cca ggt gtg tac cgc lie His Pro y Val Tyr Arg 220 Ser His Trp 10 Sn G u Phe Sy Thr Pro Leu Ala Lys Leu G u Val Hi s Lys Al a 90 Thr Phe Ar g Page 558 Asp G u G u Pr o Leu Leu Pr o Leu Hi s Hi s Asp Al a Al a Asp 110 Tyr Val Al a Phe Thr Asp Al a Gene Sequence 24-2-06.txt Thr Lys IVet Tyr Asn Al a G y Trp Al a u Lys Thr Thr Lys Leu Ile 115 120 125 Asp Thr Ala y Asp y Lys Ala y Trp u y Tyr Leu n Ala 130 135 140 IVet Arg Asn Arg Arg G n Tyr Phe I e Asn Hi s G y Al a Thr Ser Al a 145 150 155 160 Asp His G y Leu His Asp Thr Asp Thr Thr Pro Leu Ser His Lys Asp 165 170 175 Ala G n Lys Ile Leu Asp Lys y Leu Ala y Thr Ala Thr Leu Ala 180 185 190 G u IVet His Ala Phe G u Ala Asn Thr Thr Tyr Arg Phe Ala G u Iet 195 200 205 Ser G n G u Asp G y Leu Val IVet Thr IIe His Pro G y Val Tyr Arg 210 215 220 <210> 431 <211> 533 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> <223> RXN03180 <400> 431 ttc ggt gag aac aaa gat ctc atc tct gac agc agt ttc aac cgc tgg 48 Phe Gy G u Asn Lys Asp Leu Ile Ser Asp Ser Ser Phe Asn Arg Trp 1 5 10 ctg cgt acg gtt tcc ctc gga tcg acc cag gat gcc gat atg gct gca 96 Leu Arg Thr Val Ser Leu y Ser Thr G n Asp Ala Asp IVet Ala Ala 25 gct tcc aac ttg gca gcc aat tct aaa atg gcc cgc cag aac acc cgc 144 Ala Ser Asn Leu Ala Al a Asn Ser Lys IVet Ala Arg G n Asn Thr Arg 40 gat atc ctc gac gca gtc tct gat ggt ggc gtc atg ctc ggc cga aac 192 Asp Ile Leu Asp Ala Val Ser Asp Gy y Val IVet Leu y Arg Asn 55 ggt gcc cta gtg ttg gga cct gtg gtt gga act ctc cac att aaa ttc 240 Sy Ala Leu Val Leu G y Pro Val Val Gy Thr Leu His lie Lys Phe 70 75 att gcg cct ttg aac aag cgt gtg gaa aga gtc atg tac aaa act gga 288 I e Al a Pro Leu Asn Lys Arg Val G u Arg Val IVet Tyr Lys Thr G y 90 ctc tca gaa gct gct gca gct gag caa tgt gct ttg gag gat cgt ctc 336 Leu Ser G u Ala Ala Al a Ala u G n Cys Ala Leu G u Asp Arg Leu 100 105 110 cgc gaa gag atg gcc cac gct ttg tat caa tgg aat ccg gga cgc gat 384 Arg G u G u Iet Ala His Ala Leu Tyr G n Trp Asn Pro G y Arg Asp 115 120 125 Page 559 ene Sequence 24- 2- 06. t xt gaa aac t at gac ct c gt g atc aac acc ggt t cg atg aca tac gaa caa O31u Asn Tyr Asp Leu Val Ilie Asn Thr G31y Ser N/b t Thr Tyr Ou On 130 135 140 at c gt t gat ct a gt t gt g gaa act t ac gcc agg aag t at ccg ct c cac Ilie Val Asp Leu Val Val O31u Thr Tyr Ala Ar g Lys Tyr Pr o Leu Hi s 145 150 155 160 gt g aga at c at t ccg aac gga aaa gac caa t aaacat aca gt ccccgt ga Val Arg Ilie Ilie Pr o Asn G31y Lys Asp O31n 165 170 t gt <210> 432 <211> 170 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 432 Phe 03 y 03 u Asn Lys Asp Leu I I e Ser Ser Ser Phe Asn Le u A a As p G31y li1e Le u Ar g O31u I Ie 145 ValI Thr As n Le u Le u Pr o O31u O31u 115 Tyr As p I Ie Ser A a A a Le u As n A a A a Le u ValI Pr o 1 G y As n Ser Pr o Ar g A a A a I Ie 135 0Ou 0Gy Thr Ly s G31y ValI O31u O31n 1 Tyr Thr Tyr As p As p A a ValI Thr 75 ValI A a Tr p Ser Ar g 1 55 A a Ar g vb t Le u vb t Le u As n vb t 140 Ly s As p O31n Le u Hi s Tyr (31u Pr a 125 Th r Ty r Ar g A a Thr Ar g Ly s Thr Ar g Ar g (31u Le u Tr p A a Ar g As n Ph e G31y Le u As p O31n Hi s 1 <210O> 433 <21 1> 533 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 ODS <222> (510) <223> FRXA02873 <400> 433 t tc ggt gag aac aaa gat ct c at c t ct gac agc agt t tc aac cgc t gg Page 560 Phe G3 y (3 u Asn Lys Asp Leu ene Sequence 24- 2- 06. txt I Ie Se r As p Se r Se r Ph e As n Ar g Tr p 10 1 ct g Le u gct Al a g at As p ggt G31y at t I Ie ct c Le u cgc Ar g g aa (31u at c I Ie 145 ac g Thr aac As n ct c Le u ct a Le u cct Pr o g aa (31u gag (31u 115 t at Tyr g at As p 5 t cc Ser gca Al a gca Al a t tg Le u aac As n gct Al a gcc Al a ct c Le u gt t ValI ct c Le u gcc Al a gt c ValI gga G31y 70 aag Ly s gca Al a cac Hi s gt g ValI gt g ValI 150 gga G31y aat As n t ct Ser 55 cct Pr o cgt Ar g gct Al a gct Al a at c I Ie 135 g aa (31u ac c Thr 25 aaa Ly s ggt G31y gt t ValI g aa (31u c aa O31n 1 05 t at Tyr ac c Thr t ac Tyr c ag O31n at g G31y gga G31y ag a Ar g 90 t gt cys c aa O31n ggt G31y gcc Al a g at As p gcc Al a gt c ValI act Thr 75 gt c ValI gct Al a t gg Tr p t cg Ser ag g Ar g 1 55 gcc Al a cgc Ar g at g vb t ct c Le u at g vb t tt g Le u aat As n at g vb t 140 aag Ly s g at As p c ag O31n ct c Le u c ac Hi s t ac Tyr gag O31u ccg Pr o 125 ac a Thr t at Tyr at g vb t aac As n ggc O31y at t I Ie aaa Ly s g at As p 110 gga O31y t ac Tyr ccg Pr o gct Al a ac c Thr cga Ar g aaa Ly s act Thr cgt Ar g cgc Ar g g aa O31u ct c Le u gca Al a cgc Ar g aac As n tt c Ph e gga O31y ct c Le u g at As p c aa O31n c ac Hi s 1 gtg aga atc att ccg aac gga aaa gac caa Val Arg Ilie Ilie Pro Asn Oy Lys Asp O1n 165 170 t gt <210> 434 <211> 170 <212> PRT <213> Cor ynebact er i urn gi ut ani cumn <400> 434 Phe 03 y 03 u Asn Lys Asp Leu Il e Ser Asp 1 5 10 Leu Ar g Thr Val Ser Leu O31y Ser Thr O31n Al a Ser Asn Leu Al a Al a Asn Ser Lys I'b t 40 Asp Ilie Leu Asp Al a Val Ser Asp O31y O31y 55 O31y Al a Leu Val Leu O31y Pr o Val Val O31y 70 Ilie Al a Pr o Leu Asn Lys Ar g Val O31u Ar g Pag t aaacat aca gt ccccgt ga Ser As p Al a ValI ThrI ValI e 561 Se r Al a Ar g Ivbt Leu Ivbt Ph e As p O31n Le u Hi s Tyr Ar g Al a Th r Ar g Ly s Th r Leu Ser Ar g G31u 31u As n 130 1 1 e Val 145 Val Ar g G3 u A a A a 1 00 a3 u Nvbt A a 115 Tyr Asp Leu Asp Leu Val Ilie Ilie Pr o 1 65 A a A a Hi s A a Val I Ie 135 Val G31u 150 As n (1y ene Sequence 24- 2- 06. txt 90 a3 u (31n Cys Ala Leu G3 u Asp Ar g Leu 105 110 Leu Tyr (31n Tr p Asn Pr o 03 y Ar g Asp 120 125 Asn Thr a3 y Ser I'b t Thr Ty r a3 u a3 n 140 Thr Tyr Ala Ar g Lys Tyr Pro Leu Hi s 155 160 Lys Asp a3 n 1 <210O> 435 <21 1> 798 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (775) <223> RXA02292 <400> 435 t gcaccacgc cagct gcaac cct gcgcggt ggtct gggaa aaaagcgt ag gcact aaagt t ct cct gcac aat ggaggat gt tggt ggag gggat cgt cg at g gac aat gac t tt 11! I'bt Asp Asn Asp Phe g aa a31u gcg A a t ac Tyr ag a Ar g gcc A a at c I Ie gca A a ccg Pr o at c I Ie cgt Ar g c aa a31n gt a ValI at t I Ie tt t Ph e ag c Ser g at As p 120 aaa Lys5 g aa a31u gga a31y aat As n t ac Tyr ct c Le u ct c Le u t gg Tr p at g vb t gt g ValI cct Pr o cct Pr o a31y 75 acc Thr ggg a31y gaa a31u ag c Ser gca A a ac a Thr gcc A a t tc Ph e at t I Ie cca Pr a at g vb t ag c Ser c aa a31n g ac As p 45 ag c Ser aac As n t ta Le u aac As n cgc Ar g 125 ggc a31y aaa Lys5 ccc Pr a gga a31y ac c Thr g at As p t gt Cys 110 t cc Ser g at As p 15 ac a Thr gca A a ac c Thr ac g Thr at c I Ie 95 c ag a31n ggt a31y t gg Tr p gcg A a cga Ar g t gc Cys at c lIe 80 gca A a ct c Le u ggg a31y gt g ValI ct t Le u gcc A a 50 at c I Ie gag a31u gt t ValI ac c Thr g aa a31u 130 gct A a t tc Ph e 35 g aa a31u aaa Lys5 cat Hi s cgc Ar g gca A a 115 aat As n ac c Thr 20 c ac Hi s at a I Ie gcg A a gt g ValI at c I Ie 1 00 ggt a31y a31y a31y g aa a31u ct a Le u cca Pr a Ph e ggg a31y c ac Hi s gca A a ccc at t t cc at t ggc gag gat acg t gg ct g ggt gga aat gt c acc gt c Page 562 Pr o I Ie Se r I Ie (31y (3 135 gt t ggt ggc gt g agc at Val G31y G31y Val Ser 1 1 150 1 gt g gt g acc aag gat at Val Val Thr Lys As p 1 1 1 70 gcg cga gt a gt g cgg ae Ala Ar g Val Val Ar g Ly 1 85 ct g cca gaa ggt gct t c Leu Pr o 31u G31y Ala Se 200 tca cct agg ct g tca ge Ser Pr o Ar g Leu Ser a3 215 ggcactaaga aga <210> 436 <211> 225 <21 2> PRT <21 3> Cor ynebact er i urn U t e t e a As p 140 ggC G31y ccg Pr o cgt Ar g gt g ValI aat As n 220 ene Sequence 24- 2- 06. txt Thr Trp Leu G31y G31y Asn Val Thr 145 gat agg t gt gt g at t ggc gcg ggg Asp Ar g Cys Val Ilie G31y Ala G31y 1 gat gat t ct at t gct gt g ggc aac Asp Asp Ser Ilie Ala Val G31y Asn 175 180 gat gat agc cgg ct c gaa cgt t cg Asp Asp Ser Ar g Leu G31u Ar g Ser 190 195 gat gcg t tg ggg at t ct t cct aca Asp Ala Leu G31y Ilie Leu Pr o Thr 205 210 at t gcc gaa aaa t at t aaat acgca Il e A a G3 u Lys Tyr 225 gi ut ani cumn <400> 436 I'b t As p As n Asp Phe G3 u Ser Tyr Le u Thr Thr a31y Pr o Ph e Tr p a31y 145 li1e A a A a Ph e a31u Ly s Hi s Ar g A a 115 As n ValI A a (31y a31y a31u Le u Pr o Ph e a31y Hi s A a ValI Pr a 1 65 Pr o A a Tyr Ar g A a 70 li1e A a Pr a Pr a ValI 150 ValI A a a31u As n Thr li1e As n Ar g ValI I Ie 135 a31y ValI Ar g I e a3 u Lys 10 Arg a31u a31u a31n I Ie a31y 40 Val Leu Asn Il e a3 u Tyr Phe a3 y Leu 90 Ser I'b t Leu 1 Asp Asp Trp 120 Ser Ilie a31y a31y Val Ser Thr Lys Asp 1 70 Val Val Ar g 1 85 vb t ValI Pr a Pr a a31y 75 Thr a31y a31u a31u I Ie 1 55 li1e Ser A a Thr A a Ph e I Ie Pr a vb t As p 140 a31y Pr a Ser a31n As p Ser As n Le u As n Ar g 125 Thr As p As p As p Thr A a Thr Thr I Ie a31n a31y Le u Cys Ser 1 Tr p A a Ar g Cys lIe A a Le u a31y a31y ValI 1 li1e Lys Arg Asp Asp Ser Ar g 1 Page 563 ene Sequence 24- 2- 06. t xt Leu Ou Arg Ser On Leu Pro Ou Gy Ala Ser Val Asp Ala Leu Gy 195 200 205 li1e Leu Pro Thr Lys Ser Pro Ar g Leu Ser O31u Asn I Ie Al a O31u Lys 210 215 220 Ty r 225 <210O> 437 <21 1> 891 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> <221 CDS <222> (868) <223> RXA02666 <400> 437 gctcggcgac gaggaagaga agaaggacgc attcgacgac ttcgacgatt ccgacgtgga t ct tgacgat ct gagct tcg acgacgaaga t tagacgccc at c G31y t ta Le u at c I Ie ct g Le u ct c Le u c ag O31n c ac Hi s gt g ValI gt a ValI 1 50 gt c ValI CCC Pr o cgc Ar g at c I Ie aaa Ly s c ac Hi s at t I Ie ag c Ser 120 aaa Ly s g ac As p gca Al a ccc Pr o ct c Le u gt c ValI ggt G31y ggc G31y ct t Le u cga Ar g c ac Hi s at c I Ie ct c Le u aaa Ly s caa O31n ag c Ser ct t Le u 75 ggg G31y gac As p gcg Al a gaa (31u aaa Ly s 155 ct c Le u gca Al a gcc Al a ccc Pr o t tg Le u gag (31u g ac As p ct c Le u ggc G31y 140 cga Ar g gcg Al a t tc Ph e at g vb t 45 g ac As p aac As n cgc Ar g gcc Al a ac a Thr 125 gcc Al a gt g ValI gcg Al a gt c ValI ct c Le u at g vb t g ac As p gcg Al a ac c Thr 110 cca Pr a ac c Thr t cc Ser gcg Al a 15 ac g Thr ac c Thr g aa (31u ccc Pr a g ac As p 95 ccc Pr a ccc Pr a gca Al a cct Pr a ggg G31y t tg Le u t cc Ser ac c Thr g aa (31u 80 t cg Ser g at As p ggc G31y gt c ValI g at As p 1 60 at g t cg t ct aca cga I'b t Ser Ser Thr Ar g 1 cgc gga acc cgc ct C Ar g G31y Thr Ar g Leu cgt gaa cgc aca ct t Ar g O31u Ar g Thr Leu gaa agc gtc gac gaa O31u Ser Val Asp O31u t ac gcc cgc gat tt g Tyr Ala Arg Asp Leu ggg gt a cgc gt a cgg G31y Val Ar g Val Ar g gt c t gg gca ggc ct t Val Tr p Al a G31y Leu 1 00 gca at t gt c t ta at c Ala Ilie Val Leu Ilie 115 at g at t gcg cgc gt g I'b t Ilie Al a Ar g Val 130 at c cca gt a ct g cca Ilie Pr o Val Leu Pr o 145 ggc gga gt a gt t gt c G31y G31y Val Val Val 1 gac aca ccc aac cgt gca gaa ctt cgc gcc gtc caa acc cca caa ggc Page 564 Asp Thr Pro Asn Ar g Al a (31u Leu P 1 70 t tc ct g ct g t cc gaa ct t gt t gca g Phe Leu Leu Ser O31u Leu Val Al a P 185 1 ccc aac cca ggc ttc atc cca acc g Pr o Asn Pr o O31y Phe Ilie Pr o ThrA 200 205 t ac ggc gca gat gt a gt c t gc gt a c Tyr G31y Al a Asp Val Val Cys Val C 215 220 gt a aca acc ccc at t gat at g at g c Val Thr Thr Pr o Ilie Asp I'b t I'b t L 230 235 gcc gaa ccc aca at a t tt gag gt a c Ala O31u Pr o Thr Ilie Phe O31u Val F 250 t ag <210> 438 <211> 256 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn Sequence 24-2- 06. txt ~rg Al a Val O31n Thr Pr o O31n O31y 175 180 cg aat gag aaa t tc t tc gcc gac 691 ~la Asn 03 u Lys Phe Phe Al a Asp 90 195 at gac gcc agc t tg at g gaa t gg 739 osp Asp Al a Ser Leu 'b t O31u Tr p 210 aa ggc gac cca at g gcg t tt aaa 787 Sn 03 y Asp Pr o I'b t Al a Phe Lys 225 I g gca caa cgc at c acc gac gaa 835 .eu Al a O31n Ar g Ilie Thr Asp O31u 240 245 ca ggt gac taacccaatc atcccccgcg 888 ~ro G31y Asp 255 891 <400> 438 I'bt Ser Ser Ar1 Ar g (31u Tyr G31y ValI Al a Nibt I Ie 145 G31y Thr Ar g ValI Ar g Ar g Al a ValI 115 Al a ValI ValI Thr Ar g Arg Leu 20 Thr Leu As p (1u Asp Leu Val Ar g O3 y Leu 1 00 Leu I I e Ar g Val Leu Pro Val Val 1 65 I Ie Pro Val G31y G31y Pr o Leu O31u Ar g 40 li1e I Ie I Ie Leu Arg Lys 70 Leu Val Hi s O3 n Al a IlIe Hi s Asp Ser 120 Val Ar g Lys 135 Val Ser Asp 150 Asp Thr Pr o 10 I Ie Pro 25 Ser Leu Leu Val Ar g G31y G31y (1y 90 Ser Leu 1 05 Al a Ar g Val Hi s Thr I Ie Asn Arg 1 70 Il e Al a Leu Leu Al aAl aAl a 0 y Lys5 O31n Ser Le u O31y As p Al a O31u Lys5 1 Al a Al a Al a Pr o Le u O31u As p Le u O31y 140 Ar g O31u Ph e vb t As p As n Ar g Al a Thr 125 Al a ValI Le u ValI Le u vb t As p Al a Thr 110 Pr o Thr Ser Ar g Thr Thr O31u Pr o As p Pr o Pr o Al a Pr o Al a 1 O31n Th r Pr o O31n 31y Ph e L eu L eu Se r O31u L eu Va I Al a Al a As n O31u Page 565 Gene Sequence 24-2-06.txt Lys Phe Phe Ala Asp Pro Asn Pro Gy Phe lie Pro Thr Asp Asp Ala 195 200 205 Ser Leu Wet G u Trp Tyr y Al a Asp Val Val Cys Val G n y Asp 210 215 220 Pro Met Ala Phe Lys Val Thr Thr Pro lie Asp Met Met Leu Ala G n 225 230 235 240 Arg lie Thr Asp u Ala u Pro Thr Ile Phe u Val Pro y Asp 245 250 255 <210> 439 <211> 1065 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1042) <223> RXA00202 <400> 439 ctggcagcag attgtcatcg gttgtgtcat cgcgcttgcg aaacaaaacc t ctaagtaat tcctgaaagg aaattttcac gtgggcttcg atgtcatccg ctt Leu tgc Cys ggg G y cag G n ct c Leu ct c Leu act Thr gac Asp gcg Al a gct Al a cgt Ar g at c SIle cgt Ar g gtt Val aac Asn tct Ser cct Pr o 120 ttc Phe t cc Ser tct Ser ttg Leu ggt Sy gat Asp gt c Val gct Al a gt g Val gca Al a gct Al a tct Ser gct Al a gcc Al a gct Al a 75 acc Thr gt g Val gct Al a tct Ser tct Ser ggc Gy ct g Leu cag G n t cc Ser acc Thr gt g Val gt c Val gac Asp 140 gtt Val agc Ser acc Thr aag Lys gac Asp gct Al a tcg Ser 110 cgt Ar g gtt Val ttg Leu 15 gca Al a cag G n gct Al a gct Al a ggc G y 95 gt g Val t cc Ser gct Al a gac Asp acc Thr gat Asp gca Al a 80 gt g Val gaa G u t cc Ser at g bet 1 ttc Phe ggc G y aac Asn gaa G u acg Thr gt g Val gct Al a aat Asn t ac gc Tvr Al agc Ser ggt G y ccg Pr o ttg Leu cag G n at t SIle ct c Leu ggt G y 130 t cgt aaa a Arg Lys g tct gct u Ser Al a t gcg gat r Al a Asp c ttt gtg e Phe Val c gtg acc y Val Thr c aac cag a Asn n c aac cca I Asn Pro 100 c cag gct n n Ala c gag gtg y G u Val gct ggc ggc Al a G y G y 145 Page 566 gcg cag gct gct Ala G n Ala Ala Gene Sequence 24-2-06.txt gca gcc ctg gca gag gcg atc Ala Ala Leu Ala G u Al a lle 150 155 caa ggc att gcg gga tcc tct Gn G y lie Ala G y Ser Ser 170 gaa gag gag atc gct aag cat G u G u G u Ile Ala Lys His 185 acc gcc aac ttt gac cgc ggt Thr Ala Asn Phe Asp Arg y 200 ctg cag gca cac ccc aat gtg Leu G n Ala His Pro Asn Val 215 220 atg gcg ttg ggc gca atc gaa IVet Ala Leu G y Ala lle G u 230 235 gtc atc gtt gtc ggt ttc gat Val lie Val Val y Phe Asp 250 gaa gat gga cgc atg ttg gcc Su Asp y Arg IVet Leu Al a 265 gga gca aag gct gtg gaa gaa Sy Ala Lys Ala Val u u 280 gct gaa aca gag gta cca gtt Ala G u Thr G u Val Pro Val 295 300 gtc gcg gac ttc aag tagtcgg Val Ala Asp Phe Lys 310 <210> 440 <211> 314 <212> PRT <213> Cor ynebact er i um gl ut a <400> 440 IVet Tyr Ala Arg Lys Leu Il e 1 5 Phe Ser Leu Ser Ala Cys Asn G y G y Ser Al a Asp G y Ser Asn Pro Phe Phe Val G n Leu 55 G u Leu y Val Thr Leu Asn 70 Thr G n Ala Asn G n Leu Asn ggt ggc gaa Gy Gy u gca Al a gag G u gag G u 205 aag Lys gcc Al a ggc G y acc Thr gca Al a 285 gag G u t ca Ser ggc G y 190 ggc G y gcg Al a ct g Leu ace Thr gtt Val 270 gct Al a gtt Val cgt Ar g 175 at t SIle ct g Leu at c l Ie ggt G y aat Asn 255 gct Al a aag Lys gt c Val ggt G y 160 gat Asp t cc Ser gac Asp ttc Phe gct Al a 240 gat Asp cag G n ct c Leu act Thr gaa at c ctc atg ct g G u Ile Leu IVet Leu gga G y gt g Val gca Al a 210 gaa G u gct Al a ct g Leu cca Pr o cgc Ar g 290 aag Lys cag G n gct Al a 195 act Thr aac Asn ggt G y gca Al a gaa G u 275 ggt G y ct c Leu gga G y 180 aag Lys aac Asn gat Asp gaa G u gcg Al a 260 gag G u gag G u gac Asp 595 643 691 739 787 835 883 931 979 1027 1065 cga t gaaaaagt c cgt ri cum Al a Leu Arg G u Ile Thr 40 Arg Asp Val n Asn Al a Ser Al a Ser 10 Ser Ser G y Leu Ala Leu G y Al a n Asp Al a Ser 75 Val Thr Thr Page 567 Val Thr Ser a u Asp Sy Leu Al a G n Al a Al a Sy Al a Asp Thr Asp Al a Val Gene Sequence 24-2-06.txt Val Al a Asn Gy 145 G u Ar g SIle Val Al a 225 Ar g Gy G n Leu Val 305 Val Asn 115 Sy G n Leu G n Al a 195 Thr Asn Gy Al a G u 275 Gy Leu Pr o Al a Val Al a Leu 165 Phe G n Leu G u Asp 245 Val Leu Asp Asn Thr Asp Al a Al a 150 G n G u Thr Leu bet 230 Val G u Gy Al a Val 310 Asp SIle Ser 135 Al a Gy G u Al a G n 215 Al a SIle Asp Al a G u 295 Al a Asp Al a Val 105 Val Val Al a Val Al a Ser Ala G u Al a 155 Al a G y Ser 170 I le Al a Lys 185 Phe Asp Arg Hi s Pro Asn G y Al a lile 235 Val G y Phe 250 Arg IVet Leu 265 Ala Val u G u Val Pr o Phe Lys Val Pro Ser 110 Val Asp Ar g 125 Asp Asn Val 140 lie y G y Ser Al a Ser Hi s G u G y 190 Sy u y 205 Val Lys Al a 220 G u Al a Leu Asp G y Thr Al a Thr Val 270 G u Ala Al a 285 Val G u Val 300 Val G u Ser Ser Al a y G u G y 160 Arg Asp 175 Ile Ser Leu Asp lIe Phe G y Al a 240 Asn Asp 255 Al a n Lys Leu Val Thr <210> 441 <211> 963 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(940) <223> RXA02440 <400> 441 gctgttaatc acgggttgtt cagctaccgg gggagcacca cgggcaacag atggggcat aggcggagga accgtcgata cgcctcggtt ggttgtcgcg atg gtg agc cac ggc et Val Ser His G y 1 gcg ccg ggc gat act ttt tgg gat ttg gtc cga aaa ggt gct gaa gac Ala Pro Gy Asp Thr Phe Trp Asp Leu Val Arg Lys y Al a u Asp 15 gcc gcc caa aaa gac aac gtt gaa ctc cgc tat tcc tct aat ccg gaa Ala Ala n Lys Asp Asn Val G u Leu Arg Tyr Ser Ser Asn Pro G u Page 568 c 115 163 211 ene Sequence 24- 2- 06. txt at c cct gaa I Ie Pro G31u gac ggc at c Asp a3 y Il e gct caa aag A a G3 n Lys gga atg aac 31y Ib t Asn gat gaa t cc Asp G3 u Ser aac gca caa Asn A a G3 n 120 cag gaa gct a3 n a3 u A a 135 gaa acc ct g a3 u Thr Leu 1 50 ct g cag gca Leu a3 n A a ct c cag gct Leu a3 n A a aac tca gaa Asn Ser a3 u 200 acc gct att Thr A a Il e 215 t at ct g cag Tyr Leu a3 n 230 aac ggc acc Asn a31y Thr at t gt g gat Ilie Val Asp ggt ct g cga a31y Leu Ar g 280 <210> 442 t cc Ser at g vb t gt g ValI t ac Tyr gca A a gt t ValI t gt cys gt c ValI ct t Le u 1 70 gt a ValI aag Ly s g at As p t ac Tyr gt t ValI 250 ac c Thr aac As n acc Thr g at As p 75 caa a31n gga a31y t t g Le u ggt a31y aac As n 155 gct A a t ca Ser at c I Ie ggc a31y at g vb t 235 ggt a31y aac As n ct c Le u at g vb t 60 gcg A a g at As p gca A a t gt cys ggc a31y 140 ggc a31y c aa a31n at g vb t gcc A a aag Ly s 220 gcc A a ggc a31y gt t ValI gt g ValI 45 cct Pr o ggc a31y t at Tyr t cc Ser gt g ValI 125 gt g ValI at g vb t g ac As p gct A a ac c Thr 205 at c I Ie gt g ValI gga a31y g at As p c aa a31n aat As n at t I Ie gga a31y gca A a 110 at c I Ie t ct Ser g at As p cgc Ar g at t I Ie 1 90 tt t Ph e c aa a31n g at As p cga Ar g gt c ValI 270 gcc A a c aa a31n gt g ValI 80 ac a Thr gcg A a g aa a31u ggt a31y ac c Thr 1 60 at t I Ie g at As p ac a Thr gcc A a ct g Le u 240 gt g ValI at c I Ie t ca Ser gt t ValI gga a31y cgc Ar g c ag a31n tt g Le u 145 t ca Ser g at As p gcg A a aac As n at t I Ie 225 t gg Tr p t ac Tyr g at As p ct a Le u ggt a31y tt c Ph e ct t Le u ggc a31y 130 ggc a31y gt g ValI t gg Tr p gca A a gca A a 210 g at As p tt g Le u ac a Thr t ca Ser gga a31y ct c Le u tt t Ph e gcc A a 115 aac As n aaa Ly s aac As n gt t ValI g ac As p 1 c ag a31n c ag a31n gcg A a gga a31y cgc Ar g ccg Pr o aac As n ggt a31y 1 00 gag a31u t cc Ser c aa a31n t cc Ser gt g ValI 1 gct A a ct c Le u c aa a31n c ac Hi s cca Pr o 260 gt c ValI gt c ValI gct A a c aa a31n g aa a31u t cc Ser gt a ValI ac c Thr 1 gga a31y gcg A a at g vb t cca Pr a cga Ar g 245 gcc A a gct gaa gcc A a a3 u A a gtt ggg gag Val a31ya(1u 275 tgacaaaaat caagagtggg gag Page 569 Gene Sequence 24-2-06.txt <211> 280 <212> PRT <213> Corynebacter i um gl utam cum <400> 442 Net Val Ser 1 Lys Ser SIle Ser Val Sy Ar g G n Leu 145 Ser Asp Al a Asn SIle 225 Tr p Tyr G u Al a Asn Ser Sy Leu Phe Al a 115 Asn Lys Asn Val Asp 195 G n G n Al a Sy Val 275 Hi s G y Al a 5 G u Asp Al a Pro u Ile Arg Val Asp Pro Val Al a 70 Asn Al a G y Sy n Asp 100 G u u Asn Ser Ser G n Sn Val u 150 Ser Thr Leu 165 Val G y Leu 180 Al a Al a Asn Leu IVet Thr G n Pro Tyr 230 His Arg As n 245 Pro Ala li e 260 Sy u y Pr o Al a Pr o Sy G n bet G u Al a G u 135 Thr G n G n Ser Al a 215 Leu Sy Val Leu Gy G n G u 40 I le Lys Asn Ser G n 120 Al a Leu Al a Al a G u 200 l le G n Thr Asp Ar g 280 Asp Lys G n Al a Al a G u Val 105 Lys Ar g Tyr Lys Sy 185 Val Ar g Sy Thr Al a 265 Phe Asn Asn Thr Asp G n Sy Leu Sy Asn 155 Al a Ser SIle Sy et 235 Sy Asn Tr p Val Leu let Al a Asp Al a Cys Sy 140 Sy G n et Al a Lys 220 Al a Sy Val Asp G u Val Pr o Sy Tyr Ser Val 125 Val let Asp Al a Thr 205 I le Val Sy Asp Val Ar g Asn Al a Pr o bet Sy Hi s G u Leu Ser 175 Ser Asp Phe Ser Pr o 255 I e <210> 443 <211> 1482 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1459) Page 570 Gene Sequence 24-2-06.txt <223> RXN01569 <400> 443 aaggcctaga gcagaccatc gattggtacc gcgaaaacga agaacaacgt cgaagctacc tacgctaagc agggacaata ggcctggtgg cgccctgcca atg gaa tac ggt aaa 111 IVet G u Tyr Gy Lys caa G n ttc Phe cgc Ar g aac Asn gct Al a gga Gy gt a Val gt g Val ct c Leu ct c Leu 150 gat Asp aag Lys gcg Al a acc Thr at a SIle 230 acc Thr gt c Val aag Lys at g et ccg Pr o tgg Tr p caa G n aac Asn 120 aac Asn at g let aaa Lys ct c Leu ttc Phe 200 gat Asp gcc Al a cac Hi s ggc G y acc Thr ttc Phe gat Asp gat Asp at t SIle ttc Phe cat Hi s gac Asp cca Pr o 170 gt c Val gac Asp acc Thr gcc Al a acc Thr gac Asp aac Asn aac Asn aaa Lys 75 ct g Leu acc Thr cag G n tgg Tr p tgg Tr p 155 gcg Al a ggc G y gcg Al a gag G u tac Ty r 235 acc Thr aac Asn ct g Leu gcc Al a ttt Phe cgc Ar g cct Pr o gcg Al a t cc Ser 140 ccg Pr o ctt Leu gcc Al a gaa G u gct Al a 220 act Thr gac Asp cgc Ar g ggg G y 45 acc Thr gt g Val gcg Al a gac Asp ct c Leu 125 ccc Pr o ct g Leu at c SIle ggc G y ttt Phe 205 cgc Ar g gcc Al a at c SIle ggc G y ct g Leu gcc Al a t cc Ser ggc Gy gt g Val 110 gag G u gac Asp ccc Pr o gac Asp gga G y 190 gt c Val gcg Al a gtt Val gaa G u 15 tgg Tr p ccc Pr o ggc G y gt c Val tcg Ser 95 gga Gy gac Asp gcg Al a at c SIle gcc Al a 175 caa G n acg Thr tgg Tr p gac Asp G y ttc Phe gat Asp acg Thr gcg Al a agc Ser gtt Val ggc Gy cat Hi s acc Thr 160 acc Thr ct g Leu cgc Ar g aaa Lys cag G n 240 1 ct a Leu aag Lys ttt Phe act Thr gt g Val acg Thr t ac Tyr acg Thr t ac Tyr 145 gag G u ccc Pr o gga G y caa G n caa G n 225 gca Al a ct g Leu gaa G u ggc G y cgc Ar g ggt G y t ac Tyr gt c Val ct g Leu 130 gcc Al a at c SIle ct g Leu acc Thr gaa G u 210 t ac Tyr gaa G u ttc Phe tgg Tr p gtc Val at g let gtt Val aac Asn 100 cgt Ar g acc Thr gtc Val gaa G u gcc Al a 180 ct a Leu gat Asp acc Thr gac Asp Page 571 Gene Sequence 24-2-06.txt gca gca gcg Al a Al a Al a atc gcg cgc I e Al a Ar g gtc ttc gac Val Phe Asp 280 cca ct c ggc Pro Leu G y 295 acc acc gca Thr Thr Al a 310 gat ggc aat Asp G y Asn at c gca cca I e Al a Pr o gaa gac at c G u Asp Ile 360 tat ggc acc Tyr G y Thr 375 gat gtt gcc Asp Val Al a 390 agc acc gcc Ser Thr Al a aac tcc gt t Asn Ser Val ccg acc tgg Pro Thr Tr p 440 gac Asp 250 aac Asn gcg Al a t ac Tyr cgc Ar g ttt Phe 330 gt a Val gcc Al a aac Asn gca Al a t ac Tyr 410 gat Asp acc Thr atc aac gca gcg gca gtg gct SIle Asn ct c Leu gaa G u caa G n 300 t ac Tyr cgc Ar g gat Asp at c l Ie acc Thr 380 ttt Phe gcc Al a ggc G y ct c Leu Al a Al a acc Thr t cc Ser 285 t cc Ser at t SIle acc Thr gat Asp gcg Al a 365 aac Asn t cc Ser aac Asn aaa Lys aac Asn 445 ct c Leu 270 t ac Tyr aaa Lys gt g Val at g et caa G n 350 cac Hi s acc Thr gac Asp aaa Lys at c SIle 430 gac Asp Al a 255 gt g Val gat Asp gca Al a cgc Ar g aaa Lys 335 at c SIle ctt Leu ggc Gy ccc Pr o gac Asp 415 gaa G u t ac Tyr Val Ala cac Hi s gaa G u gcc Al a acc Thr 320 t cc Ser ggc Gy ttg Leu gaa G u acc Thr 400 gca Al a gcc Al a ct c Leu gt g Val aac Asn ggc G y 305 agc Ser ct c Leu cgc Ar g gaa G u ccc Pr o 385 aaa Lys gcg Al a acc Thr aag Lys aac ctc gcg Asn Leu Al a 260 t cc tca gat Ser Ser Asp 275 gca ccg ttt Ala Pro Phe 290 gac at c gga Asp Ile G y tgg gtg att Trp Val lle gac gaa cgc Asp G u Ar g 340 cta tcc ttc Leu Ser Phe 355 gtg ggt gca Val Gy Al a 370 gca agc tgg Al a Ser Trp gtt acc ggc Val Thr G y ccc cgc cca Pro Arg Pr o 420 gga t tt agc G y Phe Ser 435 gaa ct c t ca G u Leu Ser 450 acc Thr t at Tyr t cc Ser gac Asp ggc G y 325 ggc G y acc Thr gca Al a gcc Al a gt g Val 405 ct g Leu gca Al a aag Lys 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1482 tgaaaggcat catcctcgca ggt <210> 444 <211> 453 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 444 IVet G u Tyr Gy Lys G n Leu Thr Ser His Thr Thr Asp lie G u y 1 5 10 Leu Leu Val Phe Asp Phe Pro Val His Gy Asp Asn Arg G y Trp Phe 25 Page 572 Lys G u Asn Trp G n Ar g Thr Gene Sequence 24-2-06.txt Lys IVet Thr Asn Leu G y Leu Pro Asp 40 Phe Thr Val Thr Tyr Thr Tyr 145 G u Pr o Sy G n G n 225 Al a Al a Val Asn Sy 305 Ser Leu Ar g G u Pr o 385 Pr o Sy Ser Sy Pr o 115 Tyr Asn Ser Pr o Al a 195 Leu Ser Hi s Leu Ser 275 Pr o SIle Val G u Ser 355 Sy Ser G n Hi s Phe Val Sy Tyr Asn Lys 165 Ar g Ar g SIle SIle Ar g 245 Thr Tyr Ser Asp Sy 325 Sy Thr Al a Al a Asn Al a 70 Sy Val Val Leu Leu 150 Asp Lys Al a Thr SIle 230 Al a SIle Val Pr o Thr 310 Asp SIle G u Ty r Asp 390 Asn G u Al a Thr Al a Val 135 Asn Lys Val G n Ser 215 Asn Al a Al a Phe Leu 295 Thr Gy Al a Asp Gy 375 Val vet Pr o Tr p G n Asn 120 Asn bet Lys Leu Phe 200 Asp Al a Al a Ar g Asp 280 Gy Al a Asn Pr o SIle 360 Thr Al a Ser Phe Asn Trp Asp Lys Val Asp Leu 90 Lys I e Thr 105 G y Phe G n Asp His Tr p Ile Asp Trp 155 Hi s Pr o Al a 170 Val Val G y 185 Pro Asp Al a Leu Thr G u Al a Al a Tyr 235 Trp Asp Ile 250 Asp Asn Asn 265 G y Al a Al a Val Tyr G y Pr o Ar g Hi s 315 Asn Phe Val 330 Ser Val Val 345 Al a Al a G y Tyr Asn Leu Ar g Al a Val 395 Al a Phe Ar g Pr o Al a Ser 140 Pr o Leu Al a G u Al a 220 Thr Asn Leu G u G n 300 Tyr Ar g Asp SIle Thr 380 Thr Val Al a Asp Leu 125 Pr o Leu SIle Sy Phe 205 Ar g Al a Al a Thr Ser 285 Ser SIle Thr Asp Al a 365 Asn Al a Ser Sy Val 110 G u Asp Pr o Asp Sy 190 Val Al a Val Al a Leu 270 Tyr Lys Val et G n 350 Hi s Thr Sy Val Ser Sy Asp Al a SIe Al a 175 G n Thr Tr p Asp Al a 255 Val Asp Al a Ar g Lys 335 I e Leu Sy Phe Ser Asp Pro Thr 400 Page 573 ene Sequence 24- 2- 06. txt Lys Val Thr G31y Val Ser Thr Ala G31u Tyr Phe Ala Asn Lys Asp Ala 405 410 415 Ala Pro Ar g Pr o Leu Asn Ser Val Leu Asp Leu G31y Lys Ilie (31u Ala 420 425 430 Th r G31y Phe Ser Ala Pr o Thr Tr p O31n Thr Arg Leu Asn Asp Tyr Leu 435 440 445 Lys 03 u Leu Ser Lys 450 <210> 445 <211> 449 <212> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 ODS <222> (426) <223> FRXA01569 <400> 445 gca A a aat As n cca Pr o at c I Ie ac c Thr gcc A a gcc A a gt t ValI t gg Tr p cgc Ar g tt t Ph e gt a ValI gcc A a aac As n gca A a t ac Tyr g at As p 115 ac c Thr t ac Tyr cgc Ar g g at As p at c I Ie ac c Thr tt t Ph e gcc A a ggc G31y ct c Le u at t I Ie acc Thr g at As p gcg A a aac As n 70 t cc Ser aac As n aaa Ly s aac As n gt g ValI at g vb t c aa O31n c ac Hi s ac c Thr g ac As p aaa Ly s at c I Ie g ac As p 135 cgc Ar g aaa Ly s at c I Ie 40 ct t Le u ggc G31y ccc Pr o g ac As p g aa O31u 120 t ac Tyr ac c Thr t cc Ser ggc G31y Le u g aa O31u ac c Thr gca A a 1 05 gcc A a ct c Le u ag c Ser 10 ct c Le u cgc Ar g g aa O31u ccc Pr o aaa Ly s 90 gcg A a ac c Thr aag Ly s t gg Tr p g ac As p ct a Le u gt g ValI gca A a 75 gt t ValI ccc Pr a gga G31y g aa (31u gt g ValI g aa O31u t cc Ser ggt G31y ag c Ser ac c Thr cgc Ar g tt t Ph e ct c Le u 140 at t I Ie cgc Ar g t tc Ph e gca A a t gg Tr p ggc G31y cca Pr a ag c Ser 125 t ca Ser ggc G31y ggc G31y ac c Thr gca A a gcc A a gt g ValI ct g Le u 110 gca A a aag Ly s g at As p at c I Ie g aa O31u t at Tyr g at As p ag c Ser aac As n ccg Pr a ggc G31y gca A a g ac As p ggc G31y gt t ValI ac c Thr t cc Ser ac c Thr t gaaaggcat cat cct cgca ggt <210O> 446 <211> 142 <212> PRT <21 3> Cor ynebact er i urn gl ut ani curn Page 574 Gene Sequence 24 <400> 446 Ala Pro Arg His Tyr Ile Val Arg Thr Ser Trp 1 5 10 Asn Asn Phe Val Arg Thr ebt Lys Ser Leu Asp 25 Pro Ser Val Val Asp Asp n lie Gy Arg Leu 40 II e Al a Al a G y I e Al a Hi s Leu Leu G u Val 55 Thr Tyr Asn Leu Thr Asn Thr Gy G u Pro Al a 70 Ala Arg Ala Val Phe Ser Asp Pro Thr Lys Val 90 Ala G u Tyr Phe Ala Asn Lys Asp Ala Ala Pro 100 105 Val Leu Asp Leu y Lys lie G u Ala Thr y 115 120 Trp G n Thr Arg Leu Asn Asp Tyr Leu Lys G u 130 135 <210> 447 <211> 1028 <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(1021) <223> FRXA02055 <400> 447 aaggcctaga gcagaccatc gattggtacc gcgaaaacga agaacaacgt cgaagctacc tacgctaagc agggacaata -2-06. txt Val I e y G u Arg G y Ser Phe Thr G y Al a Al a Ser Trp Al a Thr G y Val Arg Pro Leu 110 Phe Ser Al a 125 Leu Ser Lys 140 Asp I e G u Tyr Asp Ser Asn Pr o caa G n ttc Phe cgc Ar g aac Asn gct Al a gga acc Thr gt c Val aag Lys at g Net ccg Pr o tgg cac Hi s ggc Gy acc Thr ttc Phe gat Asp gat acc Thr gac Asp aac Asn aac Asn aaa Lys 75 ct g acc Thr aac Asn ct g Leu gcc Al a ttt Phe cgc at c SIle ggc Gy ct g Leu gcc Al a t cc Ser ggc gaa ggc G u Gy 15 tgg ttc Trp Phe ccc gat Pro Asp ggc acg G y Thr gtc gcg Val Ala 80 tcg agc Page 575 ggcctggtgg cgccctgcca atg gaa tac ggt aaa Iet G u Tyr Gy Lys 1 cta ctg gtt ttc gat Leu Leu Val Phe Asp aag gaa aat tgg cag Lys G u Asn Trp n ttt ggc ccc gtc caa Phe y Pro Val G n act cgc ggc atg cac Thr Arg G y IVet Hi s gtg ggt tcc gtt ttc Val G y Ser Val Phe acg tac ggt aac gtc Gy Ala Trp Val Asp Gene Sequence 24- 2- 06. txt Leu Arg Ala Gy Ser Ser Thr Tyr Gy Asn Val 95 100 gta acg Val Thr gtg gca Val Ala ct c gt c Leu Val 135 ctc aac Leu Asn 150 gat aaa Asp Lys aag gtt Lys Val gcg cag Al a G n acc t ca Thr Ser 215 at a aac I e Asn 230 gca gca Al a Al a tcg cgc Ser Arg tct tcg Ser Ser cac tcg Hi s Ser 295 taagcac caa G n aac Asn 120 aac Asn at g Net aaa Lys ct c Leu ttc Phe 200 gat Asp gcc Al a gcg Al a gcg Al a acg Thr 280 gcg Al a at t SIle ttc Phe cat Hi s gac Asp cca Pr o 170 gt c Val gac Asp acc Thr gcc Al a gac Asp 250 aca Thr cgg Ar g acg Thr acc Thr cag G n tgg Tr p tgg Tr p 155 gcg Al a ggc Gy gcg Al a gag G u tac Tyr 235 at t SIle acc Thr gcg Al a gcc Al a cct Pr o gcg Al a t cc Ser 140 ccg Pr o ctt Leu gcc Al a gaa G u gct Al a 220 act Thr aac Asn t ca Ser aat Asn aat Asn 300 gac Asp ct c Leu 125 ccc Pr o ct g Leu at c SIle ggc Gy ttt Phe 205 cgc Ar g gcc Al a gca Al a ccc Pr o cct Pr o 285 cca Pr o gt g Val 110 gag G u gac Asp ccc Pr o gac Asp gga Gy 190 gt c Val gcg Al a gtt Val gcg Al a tcg Ser 270 acg Thr aag Lys gga G y gac Asp gcg Al a at c SIle gcc Al a 175 caa G n acg Thr tgg Tr p gac Asp gca Al a 255 tgc Cys at g Net cag G n gtt Val ggc G y cat Hi s acc Thr 160 acc Thr ct g Leu cgc Ar g aaa Lys cag G n 240 gt g Val acg Thr aaa Lys ccg Pr o t ac Tyr acg Thr t ac Tyr 145 gag G u ccc Pr o gga G y caa G n caa G n 225 gca Al a gct Al a tgt Cys acg Thr gcg Al a 305 gt c Val ct g Leu 130 gcc Al a at c SIle ct g Leu acc Thr gaa G u 210 t ac Tyr gaa G u acc Thr cct Pr o cac Hi s 290 aca Thr ccg Pr o 115 t ac Tyr aac Asn t cc Ser ccc Pr o gcg Al a 195 ct c Leu t cc Ser cac Hi s tcg Ser cag G n 275 cgt Ar g tcg Ser cgt Ar g acc Thr gtc Val gaa G u gcc Al a 180 ct a Leu gat Asp acc Thr gac Asp cga Ar g 260 att SIe ttt Phe 1021 1028 <210> 448 <211> 307 <212> PRT <213> Corynebacter i um gl utam cum <400> 448 lIet G u Tyr y Lys G n Leu Thr Ser His Thr Thr Asp lie G u y 1 5 10 Leu Leu Val Phe Asp Phe Pro Val His y Asp Asn Arg G y Trp Phe Page 576 Gene Sequence 24-2-06.txt IVet Thr Asn Leu Lys Phe Thr Val Thr Tyr Thr Tyr 145 G u Pr o Gy G n G n 225 Al a Al a Cys Thr Al a 305 Asn Pr o Sy Ser Sy Pr o 115 Tyr Asn Ser Pr o Al a 195 Leu Ser Hi s Ser O n 275 Ar g Ser G n G n Hi s Phe Val Sy Tyr Asn Lys 165 Ar g Ar g SIle SIle Ar g 245 Pr o vet Pr o Ar g Asn Al a 70 Sy Val Val Leu Leu 150 Asp Lys Al a Thr SIle 230 Al a Ser Ser Hi s Thr Asn G u Al a Thr Al a Val 135 Asn Lys Val G n Ser 215 Asn Al a Ar g Ser Ser 295 Lys 40 Nbet Pr o Tr p G n Asn 120 Asn vet Lys Leu Phe 200 Asp Al a Al a Al a Thr 280 Al a Ser Tr p Val Lys 105 Sy Asp SIle Hi s Val 185 Pr o Leu Al a Tr p Thr 265 Val Cys Asn Lys 75 Leu Thr G n Tr p Tr p 155 Al a Sy Al a G u Tyr 235 I le Thr Al a Al a Al a Phe Ar g Pr o Al a Ser 140 Pr o Leu Al a G u Al a 220 Thr Asn Ser Asn Asn 300 Sy Thr Val Al a Asp Leu 125 Pr o Leu SIle Sy Phe 205 Ar g Al a Al a Pr o Pr o 285 Pr o Al a Ser Sy Val 110 G u Asp Pr o Asp Sy 190 Val Al a Val Al a Ser 270 Thr Lys Gy Val Ser Sy Asp Al a SIle Al a 175 G n Thr Tr p Asp Al a 255 Cys let G n Leu Pro Asp Thr Al a Ser Val Sy Hi s Thr 160 Thr Leu Ar g Lys G n 240 Val Thr Lys Pr o <210> 449 <211> 1056 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1033) <223> RXA00825 <400> 449 Page 577 Gene Sequence 24- 2- 06. txt cccgt t catg ctgggctttg gtgcggtgat ggcaactatt tgtctgatca ttgtgagttt tagtgcacgc cgattctgag aaacaactaa agtgagccac atg cgc aca gta gtt Net Arg Thr Val Val acc Thr aag Lys ct g Leu gaa G u aag Lys tct Ser at c SIle ttt Phe gtt Val aag Lys 150 tta Leu gat Asp ct g Leu gac Asp ggg Gy 230 acc Thr ggt Gy ggc Gy aat Asn gat Asp gag G u gt a Val at c SIle t cc Ser 120 gaa Gu t cc Ser tgt Cys cac Hi s ggc Gy 200 gt c Val at c SIle gac Asp ggc Gy gag G u t cc Ser ct c Leu at t SIle cct Pr o gac Asp ggc Gy gt g Val gaa Gu cac Hi s 170 gaa G u gac Asp gt c Val ggg Gy cag G n tt c Phe gt c Val gat Asp gac Asp tt c Phe 75 ctt Leu gct Al a ggt Gy cca Pr o at t SIle 155 at c SIle gca Al a acc Thr ggt Gy gga Gy 235 ct g Leu at c SIle gtt Val gcg Al a gtt Val cac Hi s cac Hi s gcc Al a t cc Ser gt g Val 140 t ac Tyr gca Al a gga Gy aag Lys gac Asp 220 gag G u cac Hi s gga Gy gt g Val gaa G u 45 gat Asp ct g Leu gac Asp cgc Ar g at t SIle 125 gat Asp ct g Leu ccg Pr o gtt Val gt a Val 205 gt a Val cgc Ar g acc Thr t cc cat ct c Ser Hi s Leu 15 atc gat aac Ile Asp Asn gcc acc gga Al a Thr G y ttc aac gag Phe Asn G u gca gcg caa Al a Al a G n gcc gaa acc Al a G u Thr 95 cag cac ggt G n Hi s G y 110 tac ggt gag Tyr G y G u cca cat t cc Pro His Ser aac acc ttc Asn Thr Phe 160 gca aat gt t Al a Asn Val 175 gtg gcc att Val Ala lle 190 ttc ggc gac Phe G y Asp gtt cgt gct Val Arg Ala ttc aac att Phe Asn Il e 240 ctc gtg gcc Leu Val Al a 1 gtt Val ct c Leu aaa Lys ttt Phe at c SIle aac Asn gtt Val cct Pr o cct Pr o 145 cgc Ar g t ac Tyr ttc Phe ggc Gy ttc Phe 225 ggc Gy gac Asp t cc Ser ct c Leu ct a Leu gat Asp at t SIle cgc Ar g tcg Ser 130 t at Tyr cac Hi s ggc Gy gcg Al a gga Gy 210 t ac Tyr acc Thr ctt Leu cgc Ar g acc Thr gga Gy gt g Val ttg Leu aag Lys 115 gaa G u gcg Al a ct g Leu cca Pr o ct g Leu 195 aac Asn ct g Leu tct Ser ttg Leu gga Gy ttt Phe acc Thr cgc Ar g t cc Ser 100 gtt Val ttc Phe gca Al a t ac Tyr cgc Ar g 180 cga Ar g acc Thr gct Al a gtg Val act gcg gca ggt tcc Thr Al a Al a G y Ser Page 578 Gene Sequence 24-2-06.txt 250 255 260 aaa gat gat cct gaa tat gca cct gca cgt ctc ggc gat gtg cca cgc Lys Asp Asp Pro G u Tyr Ala Pro Ala Arg Leu G y Asp Val Pro Arg 265 270 275 agt gca ctc agc ttc ggc aag gcc aaa gag gtg ctt ggt tgg gag cct Ser Ala Leu Ser Phe G y Lys Ala Lys G u Val Leu y Trp G u Pro 280 285 290 gag gtg aac atc gaa caa ggt gtg gcc aag act gtg gag tac ttc cgc G u Val Asn Ile G u G n y Val Ala Lys Thr Val G u Tyr Phe Arg 295 300 305 act cac taggggaaaa tccaccacaa atc Thr His 310 <210> 450 <211> 311 <212> PRT <213> Corynebacter i um gl ut ai cum 931 979 1027 1056 <400> 450 Mvt Arg Thr Val 1 Val Asp Leu Leu Leu Ser Arg y Lys Leu Thr Phe Phe Leu G y Thr Ile Asp Val Arg Asn Ile Leu Ser 100 Val Arg Lys Val 115 Pro Ser G u Phe 130 Pro Tyr Ala Ala 145 Arg His Leu Tyr Tyr Gy Pro Arg 180 Phe Ala Leu Arg 195 Gy y Asn Thr 210 Val 5 I e Ar g Val Hi s Hi s Thr Val Pr o Ser Gy 165 G n Leu Ar g Thr G y G y Al a G u G u Al a 55 Pr o Val Ar g Thr Asp 135 Val Asp Pr o Gy Tyr 215 Hi s 25 Leu Leu Val Asp Al a 105 Ser Thr Gy Ser Gy 185 Leu Tyr Phe Val Asp Asp Phe Leu Al a Gy Pr o SIle 155 I le Al a Thr Gy SIle Val Al a Val Hi s Hi s Al a Ser Val 140 Tyr Al a Gy Lys Asp 220 Gy Val G u Asp Leu Asp Ar g SIle 125 Asp Leu Pr o Val Val 205 Val Ser I e Al a Phe Al a Al a G n 110 Tyr Pr o Asn Al a Val 190 Phe Val Hi s Asp Thr Asn Al a G u Hi s Gy Hi s Thr Asn 175 Al a Gy Ar g Phe Tyr Leu Ala Ser Gy Ou lie Gy Gy Gy Ou Arg Phe Asn lie Page 579 ene Sequence 24 225 230 235 O31y Thr Ser Val O31u Thr Ser Asp Ar g O31n Leu 245 250 Thr Ala Ala 3 y Ser Lys Asp Asp Pr o (3u Tyr 260 265 G31y Asp Val Pr o Ar g Ser Ala Leu Ser Phe G31y 275 280 Leu G31y Tr p O31u Pr o O31u Val Asn Ilie 31u O31n 290 295 Val O31u Tyr Phe Ar g Thr Hi s 305 310 <210> 451 <211> 1140 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> ODS <222> (1117) <223> RXA02054 <400> 451 cct aat gaac agccggagca ccct ggt cgt t tgcagaat a t aactct gcc agct cgcccg gacgaact aa ggt agacggc 2- 06. t xt 240 Hi s Th r Leu Val A a 255 Ala Pr o Ala Ar g Leu 270 Lys A a Lys 03 u Val 285 G31y Val Ala Lys Thr 300 ggcgcat cga caacagct ac at g act t ct t tg ct t 11! I'bt Thr Ser Leu Leu gt g ValI gt a ValI ac c Thr gt a ValI ct g Le u aac As n at c I Ie tt c Ph e gga G31y c ag O31n gca A a ct c Le u aaa Lys5 aac As n ac c Thr c ac Hi s 120 gcc A a cct Pr o aac As n g aa (31u c ac Hi s ct c Le u gt c ValI t cc Ser gga G31y gaa (31u gcc A a ggc G31y gac As p 75 aac As n ct g Le u acc Thr tt t Ph e t ac Tyr g ac As p g at As p at c I Ie g ac As p ct a Le u g at As p at c I Ie ac c Thr aat As n 45 at c I Ie ac a Thr ccc Pr o g aa (31u g aa (31u 125 ggc G31y c ac Hi s ct c Le u t gc cys gt c ValI t cc Ser gca A a 110 gt c ValI gcc A a 15 at c I Ie aaa Lys5 g at As p c ac Hi s ccg Pr o 95 gt c ValI tt c Ph e aac As n ac g Thr ggc G31y gct A a tt c Ph e tt t Ph e cgc Ar g ggc G31y 1 tt c Ph e gt g ValI ct c Le u g aa (31u gca A a gt t ValI aag Lys5 g at As p gt c ValI ct g Le u ccc Pr a tt a Le u gca A a c ac Hi s c ac Hi s ct a Le u 130 c aa O31n aaa ag c Ser g ac As p t cc Ser aac As n 1 00 aaa ct g Le u gat cca aac cgc t tc act gaa acc acc gcc t ac aag cca t cg t ct cca Page 580 Asp Pro Asn Arg 135 Phe Thr G u 140 Gene Sequence 24-2-06.txt Thr Thr Ala Tyr Lys Pro Ser Ser Pro 145 t at Tyr 150 cgc Ar g ggt Gy at t SIle cgc Ar g ct g Leu 230 gat Asp ggc G y gat Asp tgg Tr p gag G u 310 gcc Al a gca Al a ttc Phe t ac Tyr gcc Al a 200 tgg Tr p aag Lys gt g Val gac Asp cgt Ar g 280 cct Pr o acc Thr aac Asn aag Lys at c SIle 170 cac Hi s ct g Leu cac Hi s aag Lys aac Asn 250 aac Asn gcc Al a t ac Tyr gat Asp gt c Val 330 gca Al a 155 cag G n at t SIle aca Thr gt c Val at c SIle 235 aag Lys gca Al a at g bet acc Thr tgg Tr p 315 gaa G u ggg G y gca Al a gaa G u cca Pr o gat Asp 220 ggc Gy cag G n t ac Tyr gat Asp gac Asp 300 t ac Tyr gct Al a t ct gat cac Ser Asp Hi s acc at g t ct Thr IVet Ser 175 aag ttc atc Lys Phe II e 190 aaa ct t t at Lys Leu Tyr 205 gat cac aat Asp Hi s Asn gaa acc tac G u Thr Tyr gt c at c gag Val lie G u 255 gag cac gt c G u Hi s Val 270 t cc acc aag Ser Thr Lys 285 gtt gat tcc Val Asp Ser cgc gaa aac Arg u Asn acc t ac gct Thr Tyr Al a 335 ttg Leu 160 aac Asn ccc Pr o gga G y gac Asp at c SIle 240 ctt Leu gca Al a ct g Leu ggc G y gag G u 320 gt a Val tgc Cys cgc Ar g acc Thr gcc Al a 225 at c SIle at t SIle gac Asp cgc Ar g at g bet 305 gcc Al a cac Hi s t cc Ser cag G n ggc G y 210 gt c Val ggc G y tgt Cys cgc Ar g acc Thr 290 cgc Ar g tgg Tr p gca Al a aac Asn at c SIle 195 gag G u cac Hi s gcc Al a gaa G u ccc Pr o 275 gag G u aaa Lys tgg Tr p tgg Tr p aat Asn 180 acc Thr cag G n ct g Leu gac Asp ct c Leu 260 ggc G y ct c Leu ggc G y cgc Ar g 595 643 691 739 787 835 883 931 979 1027 1075 1117 1140 aag cag gga caa Lys n G y G n t aat ggaat a cggt aaacaa ct c <210> 452 <211> 339 <212> PRT <213> Corynebacter i um gl utam cum <400> 452 IVet Thr Ser Leu Leu Val Thr y y Ala y Phe lie y Ala Asn 1 5 10 Phe Val Arg G n Thr Val G u n His Pro u Tyr Thr His lie Thr 25 Val Leu Asp Lys Leu Thr Tyr Ala G y Asn Ala Asp Asn Leu Lys G y 40 Page 581 Gene Sequence 24-2-06.txt Leu Pro Asp Ser Lys Val Thr Leu lie G u G y Ile Cys Asp Ala G u Al a Val Lys Asp Lys 145 Val Cys Ar g Thr Al a 225 I e I e Asp Ar g Met 305 Al a G n Val Asp Ser Su Ser Hi s Thr Asn Leu 100 Asn Lys Arg 115 G u Leu Asp Ser Ser Pro Al a Trp I le 165 Asn Asn Tyr 180 Ile Thr Asn 195 G u G n Val His Leu Ile Al a Asp Asn 245 G u Leu IVet 260 Pro G y Hi s 275 G u Leu G y Lys G y Leu Trp Arg Pro 325 G n Val Asp Gy Hi s Pr o 135 Ser Ser Pr o Leu Asp 215 Ser Hi s Leu bet Al a 295 G n Lys Asn Thr Hi s 120 Asn Al a Phe Tyr Al a 200 Tr p Lys Val Asp Ar g 280 Pr o Thr Asp Ser Phe 105 I le Ar g Thr Gy G n 185 Gy I e Gy Asn Lys 265 Tyr Lys SIle Asp Asn Leu Thr Thr Al a 155 G n SIle Thr Val SIle 235 Lys Al a bet Thr Tr p 315 Thr Pr o G u G u 125 Thr Ser Thr Lys Lys 205 Asp G u Val G u Ser 285 Val Ar g Hi s Pr o Val Phe Al a Hi s Ser 175 I e Tyr Asn Tyr G u 255 Val Lys Ser Asn Al a Lys Asn Asn G u Ala Thr Tyr Al a Lys 335 <210> 453 <211> 909 <212> DNA <213> <220> <221> <222> <223> Cor ynebact eri um gl ut arri cum CDS (886) RXN00427 Page 582 Gene Sequence 24-2-06.txt <400> 453 gcggtcattg tgacccacaa tcgtgtggaa ttgctgcggc attccctcga ggttgttgcc aatcaaacct atccggtgaa acacat t gtg gtggtggat a atg ggg cgg atc cta Met Gy Arg lle Leu gt a Val acg Thr ttt Phe gat Asp gcc Al a gct Al a tgg Tr p gat Asp gct Al a cgc Ar g 150 ccg Pr o gat Asp gat Asp at g Net tt t ttt Phe 230 t at Tyr tcg Ser acg Thr ggc Gy tct Ser gat Asp cgg Ar g ct g Leu 120 gca Al a gat Asp ggt Gy ttc Phe gat Asp 200 cag G n tgg Tr p gt c Val acc Thr ttg Leu ccg Pr o cat Hi s gag G u cgc Ar g ggc Gy gag G u gt g Val tgt Cys 170 ccg Pr o aag Lys gga Gy ttc Phe tta Leu ttg Leu ct g Leu ggg Gy ct g Leu ttg Leu gag G u gcc Al a at t SIle 140 t at Tyr acc Thr ct g Leu ttt Phe cgc Ar g 220 gtt Val gt g Val gac Asp gcg Al a ggg Gy gag Gu ttt Phe at t SIle 110 ttg Leu gt g Val cgc Ar g gcg Al a ggg Gy 190 acc Thr ctt Leu aaa Lys gcg Al a 15 ggt Gy aac Asn gt g Val gtt Val ccg Pr o 95 gat Asp ttc Phe ccg Pr o cgt Ar g t at Tyr 175 cgg Ar g t ac Tyr ct c Leu cgg Ar g gga Gy ggc Gy gcg Al a ttg Leu tct Ser ct g Leu cca Pr o aat Asn gac Asp ttg Leu 160 ttg Leu at g Net cgc Ar g cct Pr o gat Asp 240 cgt Ar g ttt Phe tgg Tr p acg Thr gt g Val cgg Ar g aac Asn gcc Al a 130 cga Ar g cgt Ar g ccg Pr o acg Thr cgt Ar g 210 gaa Gu ccc Pr o gct Al a tgc Cys ctt Leu gt a Val ggc Gy ccg Pr o 115 ct g Leu ct g Leu t cc Ser gat Asp cag G n 195 ggc Gy t at Tyr gtt Val ttt Phe aca Thr at c SIe tgc Cys ttg Leu 100 gag G u at c SIe ttt Phe ggt Sy ggt Gy 180 t at Tyr t ac Tyr gcg Al a gtg aag gga ttc Val Lys G y Phe Page 583 Gene Sequence 24- 2- 06. t xt gag tgg ctg cgc ctg cac aaa ctg ggc cgc gac gag aaa ttc aat agg 883 G u Trp Leu Arg Leu His Lys Leu G y Arg Asp G u Lys Phe Asn Arg 250 255 260 ccc tagatcagtt ttagtagttc ctc 909 Pro <210> 454 <211> 262 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 454 Met y Arg Ile Leu Val Phe Tyr Ser Val Thr Leu G u Val Ala y 1 5 10 Asp Arg Pro Val Tyr Thr Pro Ser Arg Thr Asn Leu y Asp Gy y 25 G y Phe Ala Phe G y Phe Leu Thr Al a Leu Ala Leu y Ala Asn Ala 40 Val Trp Cys Thr Asp Asp Asp y Arg Pro G u y Pro y Val Leu 55 Lys Thr Leu Ile Asp Al a Ala Ser Arg His Asn Leu G u G u Val Ser 70 75 Pro Val Val Cys Asn Al a Asp Asp Pro G u Arg Leu Ala Phe Pro Leu 90 Arg Arg y Leu G u Trp Arg Arg IVet Arg Ser G u Leu lie Asp Pro 100 105 110 Ala Asn Pro G u Asp Asp Leu Leu Pro Gy lie Ala Ser Leu Phe Asn 115 120 125 G y Ala Leu IIe Ser Ala Tyr Ala Iet G u Arg IIe G y Val Pro Asp 130 135 140 Tyr Arg Leu Phe lie Arg G y Asp G u Val G u Tyr His Arg Arg Leu 145 150 155 160 Val Arg Ser y Leu Pro Phe y Thr Cys Leu Thr Thr Ala Tyr Leu 165 170 175 His Pro Asp G y Ser Asp G u Phe Lys Pro lie Leu G y G y Arg IVet 180 185 190 His Thr G n Tyr Pro Asp Asn Asp Phe Lys Arg Phe Phe Thr Tyr Arg 195 200 205 Asn Arg y Tyr Leu IVet Ser G n Pro Gy IVet Arg Lys Leu Leu Pro 210 215 220 Sn G u Tyr Ala Arg Phe Ala Trp Phe Phe Leu Val G n Lys Arg Asp 225 230 235 240 Val Lys G y Phe Arg G u Trp Leu Arg Leu His Lys Leu G y Arg Asp 245 250 255 G u Lys Phe Asn Arg Pro 260 <210> 455 Page 584 Gene Sequence 24-2-06.txt <211> 555 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(532) <223> FRXA00427 <400> 455 tgctgatgat ccggagcggt tggcatttcc gctgcgtcgg ggcttggagt ggcgtcggat gcgcagtgag ttgattgatc cagccaaccc ggaggatgat ttg gcc Al a at t SIle t at Tyr acc Thr ct g Leu ttt Phe cgc Ar g gtt Val ttg Leu gt g Val cgc Ar g gcg Al a ggg Gy acc Thr ctt Leu aaa Lys 120 aat Asn gac Asp ttg Leu ttg Leu at g Met cgc Ar g cct Pr o gat Asp gcc Al a cga Ar g cgt Ar g ccg Pr o acg Thr cgt Ar g gaa G u aag Lys ct g Leu ct g Leu t cc Ser 45 gat Asp cag G n ggc Gy t at Tyr gga Gy 125 at c SIle ttt Phe ggt Gy ggt Gy t at Tyr t ac Tyr gcg Al a 110 ttc Phe Leu 1 gct tat Al a Tyr cgc ggc Arg G y ccg ttt Pro Phe gat gag Asp u gat aat Asp Asn 80 atg agc et Ser ttt gcg Phe Al a gag tgg Su Trp ctg ccg ggc atc Leu Pro Gy lie gca atg gag cgc Ala Vet G u Arg gat gag gtg gag Asp u Val u ggt acg tgt ttg G y Thr Cys Leu ttc aag ccg att Phe Lys Pro lle gat ttc aag agg Asp Phe Lys Arg cag ccg gga atg Sn Pro Gy bet 100 tgg ttc ttc ctg Trp Phe Phe Leu 115 ctg cgc ctg cac Leu Arg Leu His 130 aaa ctg ggc cgc gac gag aaa ttc aat agg ccc tagatcagtt ttagtagttc 552 Lys Leu y Arg Asp G u Lys Phe Asn Arg Pro 135 140 ct c 555 <210> 456 <211> 144 <212> PRT <213> Corynebacter i um gl ut am cum <400> 456 Leu Leu Pro Gy lie Ala Ser Leu Phe Asn Gy Ala Leu Ile Ser Ala 1 5 10 Tyr Ala Iet u Arg Ile Gy Val Pro Asp Tyr Arg Leu Phe lie Arg 25 G y Asp G u Val G u Tyr His Arg Arg Leu Val Arg Ser G y Leu Pro Page 585 Ph e G31u As n Ser A a Tr p Thr Ly s Ph e Pr o Ph e 115 Ar g Le u I Ie Ar g vb t Le u Hi s Thr G31y Ph e Ly s O31n Le u 135 ene Sequence 24- 2- 06. t xt 40 Ala Tyr Leu Hi s Pro Asp G31y (31y Ar g N/bt Hi s Th r (31n Ty r Thr Ty r Ar g Asn Arg (31y Ty r 90 Leu Leu Pro O31n O31u Ty r Ala 105 110 Lys Ar g As p Val Lys G31y Phe 120 125 (31y Ar g As p O31u Lys Phe As n 140 Ser Pr o Le u Ar g Ar g Ar g <210O> 457 <21 1> 507 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (484) <223> RXA00327 <400> 457 aacgatt t tc gccgt cgcaa ccgt gat cac cggt cgctt t ctagggggcc aggcgcggga tgattgggtt tgtcacgccg ccggcat cgt gt cgt cgcgg at g at t cag gcg gcg 11! I'bt Ilie On Ala Ala tt g Le u gt t ValI gcg A a at g vb t at g vb t ggC G31y ggC (31y at c I Ie gt c ValI tt c Ph e gcc A a gcc A a gcc A a gt g ValI ct c Le u gcc A a tt c Ph e ac c Thr c aa O31n ac c Thr ct g Le u tt t Ph e at c I Ie g at As p ggc G31y at c lIe 75 t gg Tr p gca A a t ta Le u at g vb t ac g Thr at g vb t at c I Ie t cg Ser ggc (31y t tc Ph e t tc Ph e at c I Ie 45 gcc A a gga G31y aat As n at g gct A a gcg A a t gc cys aac As n tt t Ph e t tc Ph e at c I Ie 110 g at As p 15 at t I Ie g aa O31u at g vb t t ta Le u c ag O31n 95 ggg (31y cgc Ar g t cc Ser aaa Lys5 ag c Ser ct c Le u gt g ValI ct t Le u 1 ct g Le u tt t Ph e ct c Le u gcc A a g ac As p gcg A a gca A a t cc Ser ag c Ser g ac As p t ac Tyr t gg Tr p t gg Tr p gt c ValI ccg Pr o ccc Pr o cgc Ar g t gc cys gcc A a ggt G31y 1 00 ct c Le u Page 586 Gene Sequence 24- 2- 06. txt ctg cag cgt cga aaa aat att gct ttt cga cgc taaaacccga ccgtaaccgc 504 Leu On Arg Arg Lys Asn lie Ala Phe Arg Arg 120 125 tag 507 <210> 458 <211> 128 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 458 IVet I e G n Ala Ala Leu Trp Ile Val Leu Phe Leu Phe Ala Asp Arg 1 5 10 Leu Ser Asn Pro Leu Val Phe Val Ser Ala lie IVet Phe Ala lie Ser 25 Phe Ser Ser Pro Val Ala Asn Phe y Phe Asp Thr lie Cys u Lys 40 Leu Asp Arg Arg Val IVet Val Al a y Thr Gy IVet Ala Asn IVet Ser 55 Ala Tyr Ile Cys Ala IVet Leu Ala Thr n lie lie y Phe Leu Leu 70 75 Asp Trp Asn Ala Asp G y His Ala Tyr Thr Trp Ser Asn Phe G n Val 90 Al a Trp Leu G y Leu G y Al a Val Trp Leu Al a G y IVet I e G y Leu 100 105 110 Ala Val Cys Leu Leu Leu G n Arg Arg Lys Asn lie Ala Phe Arg Arg 115 120 125 <210> 459 <211> 615 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(592) <223> RXA00328 <400> 459 gtcacgggat ttgttgtagc ccgactcaag ggtggaccgg gccgcagatt actcgcccag attaggtcta ccaaggactt ttaaagatcg ggtaaaacct gtg ggc gtt gaa agg 115 Val Gy Val G u Arg 1 ggc aag gtc tct gcc aag gca cta gtt gtc tgg ttg acg gca atg tgc 163 G y Lys Val Ser Ala Lys Ala Leu Val Val Trp Leu Thr Ala et Cys 15 gtg tac atc gtg gcc att gct ggt cga aca tca ttt ggt gtc gcc gga 211 Val Tyr Ile Val Ala I le Ala y Arg Thr Ser Phe y Val Ala y 30 gtg cat gcg atc gat cgc ttt gat atc gac gcc tcc cgg ttg gcc gtg 259 Page 587 ene Sequence 24- 2- 06. t xt Val His Ala lie Asp Arg Phe As p I Ie Asp Al a Ser Ar g Leu Al a Val 45 t t cact t ct gt t cag gt t gga gt ct at gt t ct cgcg cag at t ccc at g 307 Phe Thr Ser Val O31n Val G31y Val Ty r Val Leu Al a O31n I Ie Pr o l'b t 60 ggc at gct ggt ggac aggt t cgac gcc cgg aaa ct ct t gct ggct ggc 355 O31y l'b t Leu Val As p Ar g Phe As p Al a Ar g Lys Leu Leu Leu Al a O31y 75 80 gct t t aat t t t ggca gct ggc cag ct cat t t t gggt t t cact gat t ct 403 Al a Leu I I e Leu Al a Al a G3 y G3 n Leu I I e Leu G3 y Phe Thr As p Ser 95 100 t at at gat cgcc at t t tt gcc cga gt gct catc agt gt t ggc gat t ct 451 Ty r N/b t I Ie Al a I Ie Phe Al a Ar g Val Leu I Ie Ser Val G31y As p Ser 105 110 115 t ct gca t tt t tg t cc gt g at g cga ct g t tg ccc aac t gg t tc ccg at g 499 Ser Al a Phe Leu Ser Val l'b t Ar g Leu Leu Pr o As n Tr p Phe Pr o l'b t 120 125 130 t ct t gg aca cct gt g t tg cag cag ct c acg ggc gct t tt ggc t tt gt g 547 Ser Tr p Thr Pr o Val Leu O31n O31n Leu Thr G31y Al a Phe G31y Phe Val 135 140 145 ggg cag t tt t ct ccg cgg tgc cgt t tt t gc aca tac tca aca cct 592 G31y O31n Phe Ser Pr o Ar g Cys Ar g Phe Cys Thr Ty r Ser Thr Pr o 150 155 160 t agggt ggac aat tcct t tc gca 615 <210O> 460 <21 1> 164 <21 2> PRT <21 3> Cor ynebact er i urn gI ut anm cumn <400> 460 Va 1G31y Val O31u Ar g G31y Lys Val Ser Al a Lys Al a Leu Val Val Tr p 1 5 10 Leu Thr Al a N/b t Cys Val Tyr I Ie Val Al a I Ie Al a G31y Ar g Thr Ser 25 Phe (1y Val Al a 1y Val Hi sAl a IIe As pAr g Phe Asp I Ie Asp Al a 40 Ser Ar g Leu Al a Val Phe Thr Ser Val 03 n Val 03 y ValI Tyr Val Leu 55 Al a O31n IIe Pr o 'b t G31y l'b t Leu Val As p Ar g Phe Asp Al a Ar g Lys 70 75 Leu Leu Leu Al a 03 y Al a Leu I I e Leu Al a Al a 03 y 03 n Leu I I e Leu 90 O31y Phe Thr As p Ser Ty r N/b t I Ie Al a I Ie Phe Al a Ar g Val Leu I Ie 100 105 110 Ser Val 03 y As p Ser Ser Al a Phe Leu Ser Val l'b t Ar g Leu Leu Pr o 115 120 125 As n Tr p Phe Pr o l'b t Ser Tr p Thr Pr o Val Leu O31n O31n Leu Thr O31y 130 135 140 Al a Phe O31y Phe Val O31y O31n Phe Ser Pr o Ar g Cys Ar g Phe Cys Thr Page 588 ene Sequence 24- 2- 06. txt 1 145 Tyr Ser Thr Pro <210O> 461 <21 1> 1347 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1324) <223> RXA00329 <400> 461 tgtgaagaac aatgatcaac ggccttcgcc aagaccgcat ccgcgccct c at agt t t ggg cagcagcct g t ct t gt gt at cccagagaga aagtaactac ag c Ser c ag O31n gt g ValI ggt G31y c ag O31n cgc Ar g cgc Ar g c ag O31n cct Pr o ag c Ser 1 50 gt t ValI ac c Thr g ac As p gcg A a cgg Ar g at t I Ie tt t Ph e ct g Le u act Thr 120 at g vb t ggt G31y g at As p tt t Ph e ac a Thr gct A a ct c Le u ggc G31y ggc G31y tt t Ph e t gc cys tt g Le u gt g ValI cca Pr o 1 70 gt g ValI at t I Ie at t I Ie gcc A a ac g Thr ggc G31y Ph e ggc G31y ggt G31y 140 gca A a cct Pr o gct A a gca A a ccg Pr a 45 at t I Ie g at As p g at As p cca Pr a c ag O31n 125 gcg A a ct c Le u c ag O31n gga O31y gt a ValI at g vb t ggt O31y ag c Ser gca A a ct g Le u 110 tt g Le u c ag O31n at t I Ie cca Pr a gt g ValI 15 tt c Ph e ggc O31y gct A a t at Tyr t cg Ser 95 aag Ly s ggc O31y ggt O31y gcg A a aaa Ly s 1 75 gag O31u act Thr at t I Ie tt g Le u t cc Ser 80 at c I Ie c ac Hi s c ag O31n t gg Tr p at c I Ie 1 60 cct Pr a at g vb t 1 gcc A a t ct Ser tt g Le u gt g ValI at c I Ie tt c Ph e ac c Thr tt c Ph e cct Pr a 145 gca A a gt c ValI gca gcc at A a A a II at t I Ie gt t ValI at c I Ie at g vb t gcg A a ct t Le u cct Pr a tt t Ph e 130 gt g ValI gcg A a g aa O31u g at As p c ag O31n g at As p ggt O31y at t I Ie t cg Ser at t I Ie 115 t ct Ser gcg A a ct g Le u t ca Ser cg Ar 2 gt Va aa Ly at I I at I I gt Va 10 t t Ph gc A t t Ph gt Va gc A 18 t acc e Thr t tt t g Phe 0 t ggt b 31y g tt t s Phe c ggc e (1y t gcc e A a t at g I lb t 0 c acg e Thr g gt g a Val t gt c e Val g gcc I Ala 1 t cag a (1n 0 gaa gca gat aaa cca agc ctg cgc gcg agt t tg aag t tg at t gt c cgc Page 589 G u Al a Asp Lys Pro Ser 185 Gene Sequence 24- 2- 06. txt Leu Arg Ala Ser Leu Lys Leu Ile Val Arg 190 195 aat Asn tgg Tr p ggc Gy 230 acg Thr cgc Ar g caa G n ttg Leu gct Al a 310 at t SIle tcg Ser cat Hi s at t SIle ct c Leu 390 aag Lys at t SIle 200 acc Thr gga Gy tgc Cys ggg Gy gca Al a 280 gct Al a ggt Sy gct Al a gtt Val agc Ser 360 gt c Val ggt Gy ttt Phe tgg Tr p ttc Phe tct Ser gt g Val 250 cgc Ar g tgg Tr p at c l Ie ggc G y ggc G y 330 gcg Al a t at Tyr tgg Tr p ccg Pr o cag G n t cc Ser gca Al a 235 gt a Val cgt Ar g ct g Leu tt g Leu ttt Phe 315 acg Thr cag G n act Thr gcc Al a ggc G3y 395 ggt G y at g Met 220 acg Thr tcg Ser gac Asp gt g Val gt c Val 300 gac Asp gga G y gtt Val tgg Tr p gt c Val 380 cgc ttc Phe 205 at g Net acg Thr gcg Al a gt g Val ttc Phe 285 aac Asn acc Thr ct g Leu at g et gt g Val 365 gga aay aga ttc Phe tgg Tr p gct Al a cca Pr o gt c Val 270 ttg Leu at c SIle at t SIle gca Al a ggg G y 350 gac Asp gt c Val tta at t SIle ggc Gy ggt Gy at c SIle 255 gcc Al a gcc Al a gt c Val cgt Ar g aac Asn 335 ttc Phe ttc Phe acg Thr ct c cac Hi s gt c Val ttg Leu 240 at c SIle at t l Ie t cc Ser at g let gag G u 320 at g Net ctt Leu cgt Ar g gga Gy gcc Al a 400 t ac Tyr ccg Pr o 225 gt g Val gga Gy gcg Al a gat Asp ggt G y 305 cgc Ar g ggt G y ctt Leu ttt Phe ttt Phe 385 cag G n gt a Val 210 ct g Leu ttg Leu at a SIle ct g Leu gca Al a 290 ct g Leu ct a Leu gga G y gat Asp ggt G y 370 gtt Val at t SIle ttg Leu at g Net agc Ser at t SIle tcg Ser 275 cct Pr o act Thr gat Asp ttc Phe cac Hi s 355 ttc Phe gt a Val agg Ar g at g Net act Thr at c SIle t cc Ser 260 ttt Phe cgt Ar g act Thr cgc Ar g ttg Leu 340 agc Ser ctt Leu gcc Al a tct Ser gt g Val ttg Leu aac Asn 245 gca Al a gtt Val ggt Gy gcg Al a aag Lys 325 t ca Ser gcg Al a gcg Al a cga Ar g acc Thr 405 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1347 Arg Arg Leu Leu taaagatcgg gtaaaacctg tgg <210> 462 <211> 408 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 462 Iet Ala Ala l e Thr Ser Arg Thr Ser Phe y Val Ala G y Val u 1 5 10 Page 590 Gene Sequence 24-2-06.txt Al a Ser Leu Val I le Phe Thr Phe Pr o 145 Al a Val Lys Tyr Pr o 225 Val Sy Al a Asp Sy 305 Ar g Sy Leu Asp G n Asp Sy SIle Ser SIle 115 Ser Al a Leu Ser SIle 195 Leu vet Ser SIle Ser 275 Pr o Thr Asp Phe Hi s 355 Phe 0 n Val Asp Al a Thr Arg Ile Ala Val Phe Thr Sy Phe Sy Al a bet Thr Val Val Al a 165 G n Ar g Val Leu Asn 245 Al a Val Sy Al a Lys 325 Ser Al a Tyr Pr o Leu Val SI e Leu Phe 135 Leu Ar g Al a Pr o G n 215 Let Leu Leu Ser bet 295 Ser Leu bet Gy Al a 40 Ar g SIle Phe Leu Thr 120 Met Gy Asp Asp SIle 200 Thr Gy Cys Gy Al a 280 Al a Gy Al a Val Ser 360 Phe Lys Leu SIle Pr o 105 Thr Al a Ser Thr Lys 185 Thr Val Leu bet Tyr 265 Val l Ie Tyr Al a Al a 345 Thr Al a Leu Gy Gy 90 Phe Cys Leu Val Pr o 170 Pr o Tr p Phe Ser Val 250 Ar g Tr p SIle Gy Gy 330 Al a Tyr G n Leu Phe Al a Tr p Leu Leu Val 155 Asp Ser G n Ser Al a 235 Val Ar g Leu Leu Phe 315 Thr G n Thr SIle Al a Thr Sy Phe Sy Sy 140 Al a Pr o Leu Sy et 220 Thr Ser Asp Val Val 300 Asp Sy Val Tr p Pr o l e Asp Asp Pr o G n 125 Al a Leu G n Ar g Phe 205 vet Thr Al a Val Phe 285 Asn Thr Leu bet Val 365 bet Sy Ser Al a Leu 110 Leu G n SIle Pr o Al a 190 Phe Tr p Al a Pr o Val 270 Leu SIle SIle Al a Sy 350 Asp Sy Al a Tyr Ser Lys Sy Sy Al a Lys 175 Ser SIe Sy Gy I e 255 Al a Al a Val Ar g Asn 335 Phe Phe Phe y Phe Leu Ala lie Leu Val Thr Trp Ala Val y Val Thr y Page 591 Gene Sequence 24-2-06.txt 370 375 380 Phe Val Val Ala Arg Leu Lys Gy Gy Pro Gy Arg Arg Leu Leu Ala 385 390 395 400 Sn Ile Arg Ser Thr Lys Asp Phe 405 <210> 463 <211> 2265 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(2242) <223> RXN01554 <400> 463 tggcttaaac cttatttgta gttgtcaata aatatgaaat tcct t agcaa ct t gt t t aaa tagacgtata aacaagtttg aaaaaggaag gttatccatc ttg aaa aag cat Leu Lys Lys His acc Thr ggt Gy gct Al a gag G u agc Ser aag Lys at c l Ie gtt Val at c SIle gca Al a 150 at g bet gcc Al a agt Ser gat Asp gct Al a aaa Lys acc Thr gat Asp cct Pr o 120 act Thr ctt Leu aac Asn acc Thr gcc Al a tct Ser aaa Lys ct c Leu acc Thr gt c Val gct Al a aaa Lys acc Thr gaa G u gcc Al a gtt Val gcg Al a ggg Gy t at Tyr 75 act Thr aac Asn cca Pr o at t SIle ggt Gy 155 cag G n gt g Val tct Ser ccg Pr o tgg Tr p acc Thr cag G n cgc Ar g ggt Gy gct Al a 140 ggc Gy gcg Al a acg gct ttt Thr Al a Phe 15 gct gtt gcc Al a Val Al a aca t gg gt g Thr Trp Val att ttc cgc I e Phe Ar g gat gct tta Asp Al a Leu 80 cac tgc gt t Hi s Cys Val 95 aag ct c aat Lys Leu Asn 110 ttt gaa aag Phe G u Lys gga gag ttc G y G u Phe gaa ggt gt c G u G y Val 160 t cc tca gca Ser Ser Al a 1 t ca Ser caa G n gaa G u cag G n agt Ser gac Asp gac Asp ttc Phe gac Asp 145 cga Ar g acg Thr gt g Val gag G u cca Pr o at c SIle gaa G u agc Ser acc Thr 130 ctt Leu agt Ser gct Al a gct Al a ttt Phe gat Asp gaa G u gac Asp acc Thr 115 agt Ser tct Ser gcc Al a gcg Al a aat Asn gat Asp t at Tyr gat Asp ttc Phe 100 gcg Al a gcg Al a gt c Val at t l Ie gtg Val ctt Leu cca Pr o t cc Ser ggc G y ggc G y gcg Al a caa G n cgc Ar g act Thr tgg Tr p 165 acc aac aat Thr Asn Asn ggc ct c G y Leu Page 592 Gene Sequence 24-2-06.txt 170 175 180 tac gga gaa cta gac ctg gta gag cac ttt tct tac gat ctt cgc tcg 691 Tyr G y G u Leu Asp Leu Val G u His Phe Ser Tyr Asp Leu Arg Ser 185 190 195 cca tgg tct cca tca aac acc cac ttg ggt tgt gat cct gaa agt gtc 739 Pro Trp Ser Pro Ser Asn Thr His Leu G y Cys Asp Pro G u Ser Val 200 205 210 aac ggc acc aac cgt gca cct cgt gaa ctt aaa cta gat gag tca ctc 787 Asn y Thr Asn Arg Al a Pro Arg G u Leu Lys Leu Asp u Ser Leu 215 220 225 gat ggc gtg gag cac acc tgg act gtg agc acc acc cgc gac ggc gtt 835 Asp G y Val G u His Thr Trp Thr Val Ser Thr Thr Arg Asp G y Val 230 235 240 245 gag tac ttc att gat gat gag gcg att aac cgc cag tca tgg cgc aac 883 Su Tyr Phe Ile Asp Asp u Ala lle Asn Arg n Ser Trp Arg Asn 250 255 260 gat gtc act ttg ggg cat gcc gaa att gat gat ttc ggg atc tcc gcg 931 Asp Val Thr Leu G y His Ala u lle Asp Asp Phe G y IIe Ser Ala 265 270 275 cag acg ttt gat gag atc gtc gac cgc gaa tgg act ctc act ctt aat 979 G n Thr Phe Asp G u Ile Val Asp Arg G u Trp Thr Leu Thr Leu Asn 280 285 290 caa aag gta gaa agc gcc gac tgg gca aaa cca cgt tcc tct gag gaa 1027 G n Lys Val u Ser Al a Asp Trp Ala Lys Pro Arg Ser Ser G u G u 295 300 305 gat ttc cca gtc cgg tcc atg gtg att gac cgc atc gag gtc acc gga 1075 Asp Phe Pro Val Arg Ser IVet Val lie Asp Arg lie G u Val Thr y 310 315 320 325 tct ccc gca gta tct gaa gac acc ccc atg cca gat acc acc cag ctt 1123 Ser Pro Ala Val Ser G u Asp Thr Pro IVet Pro Asp Thr Thr G n Leu 330 335 340 ttg acc caa gac act ctg gaa tac ctc ggt cgc atg cca gtg ctg gaa 1171 Leu Thr G n Asp Thr Leu G u Tyr Leu y Arg IVet Pro Val Leu G u 345 350 355 cgc tac gag cca gca agt gct gat ttt gcc gat ggc cgc agg cct tcc 1219 Arg Tyr G u Pro Ala Ser Ala Asp Phe Ala Asp y Arg Arg Pro Ser 360 365 370 tgg aac tac ttc aat ttg aag gaa tcg tgg cag aat cca gaa ctc gag 1267 Trp Asn Tyr Phe Asn Leu Lys u Ser Trp G n Asn Pro G u Leu G u 375 380 385 caa cgc cca gaa gct gtc gaa ttc gtt gat gga cgc atg gat atc gtg 1315 Sn Arg Pro u Ala Val u Phe Val Asp y Arg IVet Asp lie Val 390 395 400 405 acc cgt cgc cac tgt ctg gcc acc act gat gac atc gcc act ccg gaa 1363 Thr Arg Arg His Cys Leu Ala Thr Thr Asp Asp Ile Ala Thr Pro G u 410 415 420 aac gca cag gag gaa ccg tgc gcg ccg ggt gag gtg aca cgc tac agc 1411 Asn Al a n u G u Pro Cys Ala Pro y G u Val Thr Arg Tyr Ser 425 430 435 tca gcg cgt gtc cac ctt cca gag atc ccc gcc ggc aac ttc cgg ctc 1459 Ser Ala Arg Val His Leu Pro G u lie Pro Ala G y Asn Phe Arg Leu Page 593 Gene Sequence 24-2-06.txt acc Thr ccc Pr o 470 gga Gy gt g Val cca Pr o gat Asp acc Thr 550 aac Asn ttg Leu at t SIle gcg Al a cat Hi s 630 gac Asp gat Asp acc Thr aca Thr 440 cgc Ar g at c I le cct Pr o acc Thr at g Met 520 cat Hi s gac Asp gtt Val gaa G u aac Asn 600 gac Asp gca Al a gcg Al a agc Ser agc Ser 680 gct Al a cgg Ar g at g bet ggt Gy 490 t ac Tyr ctt Leu tgg Tr p aag Lys cca Pr o 570 gaa G u at g Met aac Asn gat Asp gaa G u 650 ct g Leu at c SIle aat Asn gcg Al a cag G n 475 gaa G u tcg Ser cgc Ar g ggc Gy gca Al a 555 gcc Al a tac Ty r gt g Val gag G u at c SIle 635 at c SIle gag G u tcg Ser caa G n cag G n 460 aac Asn ct g Leu gca Al a caa G n gt c Val 540 gt a Val gct Al a cgt Ar g gag gcg Al a 620 gag G u cca Pr o gtt Val ttg Leu gaa G u 700 445 t cc Ser aat Asn gat Asp gt a Val at g bet 525 gaa G u act Thr gca Al a gaa G u cag G n 605 ttc Phe tct Ser gac Asp ggt Gy ttc Phe 685 gca Al a gaa G u acc Thr at t SIle cac Hi s 510 gaa G u gt c Val t cc Ser cct Pr o gt c Val 590 tct Ser cca Pr o gac Asp aat Asn tcg Ser 670 acc Thr ttg gag G u aac Asn act Thr 495 ctt Leu at g tet ttc Phe tct Ser ctt Leu 575 at c SIle ggc Gy gaa G u tct Ser gtt Val 655 acc Thr gcg Al a cag ctt Leu ttc Phe 480 gag G u gga Gy gaa G u gac Asp ggc Gy 560 cgc Ar g ggg Gy aaa Lys cac Hi s gt g Val 640 ggt Gy gga Gy ttg Leu aat 450 gac Asp gct Al a t ac Tyr gct Al a t cc Ser 530 cag G n gat Asp gcg Al a cct Pr o agc Ser 610 ttc Phe aat Asn gaa G u tct Ser tcg Ser 690 at t SIle ggc G y gac Asp agc Ser ggc Sy 515 at g Net at c SIle gt c Val cac Hi s tgg Tr p 595 tgg Tr p caa G n gt a Val gac Asp aca Thr 675 ct g Leu aat Asn gt c Val aac Asn tct Ser 500 aac Asn ttt Phe gt a Val ttt Phe ttc Phe 580 cac Hi s at t SIe at t SIe tgg Tr p t ct Ser 660 gct Al a gt c Val cag G n 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 2265 Leu G n Asn atg aga cag ttc aag lIet Arg G n Phe Lys taatccttgt gggcgctttg tct Page 594 Gene Sequence 24-2-06.txt 710 <210> 464 <211> 714 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 464 Leu 1 Ser G n G u G n Ser Asp Asp Phe Asp 145 Ar g Thr Tyr Asp Leu 225 Thr G n Phe Thr Ar g 305 Lys Lys Hi s Thr Al a Al a Val Al a Asn G u Phe Asp Pro Asp Tyr Ile G u Asp G u Asp Phe 100 Ser Thr Al a 115 Thr Ser Al a 130 Leu Ser Val Ser Al a li e Asn Asn G y 180 Asp Leu Arg 195 Pro u Ser 210 Asp G u Ser Arg Asp G y Ser Trp Ar g 260 G y Ile Ser 275 Leu Thr Leu 290 Ser Ser G u Val 5 Leu Pr o Ser Sy Sy Al a G n Ar g Thr Tr p 165 Leu Ser Val Leu Val 245 Asn Al a Asn G u Thr Ser Ala Val Thr 10 Sy Leu Ser Ile Al a 25 Ala Pro Asp Leu Ser 40 G u Asp Ala Leu Lys 55 Ser Asp Lys Val Leu 70 Lys Leu Thr Ile Thr 90 l e Ser Asp Pro Val 105 Val G u Pro Cys Ala 120 Ile Val Thr Pro Lys 135 Ala Thr Leu Asn Thr 150 Ibet n Asn G y u 170 Tyr G y G u Leu Asp 185 Pro Trp Ser Pro Ser 200 Asn y Thr Asn Arg 215 Asp G y Val G u Hi s 230 G u Tyr Phe Ile Asp 250 Asp Val Thr Leu G y 265 G n Thr Phe Asp G u 280 G n Lys Val u Ser 295 Asp Phe Pro Val Arg 310 Al a Val Al a Sy Tyr Thr Asn Pr o SIle Sy 155 G n Leu Asn Al a Thr 235 Asp Hi s SIle Al a Val Ser Pr o Tr p Thr G n Ar g Sy Al a 140 Sy Al a Val Thr Pr o 220 Tr p G u Al a Val Asp 300 Val Pr o Tyr Asn G u Ar g Sy G n 125 Ar g Val Cys G u Hi s 205 Ar g Thr Al a G u Asp 285 Tr p Thr Al a Thr SIle Asp Hi s Lys 110 Phe Sy G u Ser Hi s 190 Leu G u Val l Ie SIle 270 Ar g Al a Al a Val Tr p Phe Al a Cys Leu G u G u Sy Ser 175 Phe Sy Leu Ser Asn 255 Asp G u Lys Ser Iet Val lie Asp 315 Page 595 Gene Sequence 24-2-06.txt lie G u Val Thr Asp let Gy Asn 385 Ar g SIle Val Gy Val 465 Cys Phe Cys G u Gy 545 Lys Pr o G n Asp Ar g 625 Asp SIle Thr Val 355 Ar g G u Asp Thr Ar g 435 Phe Gy Asp Ser Gy 515 let SIle Val Hi s Tr p 595 Tr p G n Val Asp Gy 325 Leu G u Ser G u Val 405 G u Ser Leu Ar g Asp 485 Ar g Ar g Gy Phe Gy 565 Lys Leu Thr Asp Pr o 645 Asp Thr Tyr Asn 375 Ar g Ar g Al a Al a Val 455 Al a Ar g Asn G u Tr p 535 I le Ser Ser Leu Val 615 Val Al a Gy G n G u 360 Tyr Pr o Ar g G n Ar g 440 Ar g l Ie Pr o Thr let 520 Hi s Asp Val G u Asn 600 Asp Al a Al a Ser Ser Pro Ala Val Ser u Asp Thr Pro IVet Pro Asp 345 Pr o Phe G u Hi s G u 425 Val Al a Tr p Tyr G n 505 Lys Asp Gy Thr G u 585 Thr Asn Val Asn Asp 665 330 Thr Al a Asn Al a Cys 410 G u Hi s Ar g let Gy 490 Tyr Leu Tr p Lys Pr o 570 G u et Asn Asp G u 650 Leu Leu Ser Leu Val 395 Leu Pr o Leu Al a G n 475 G u Ser Ar g Sy Al a 555 Al a Tyr Val G u SIle 635 I le G u Tyr Asp 365 G u Phe Thr Al a G u 445 Ser Asn Asp Val let 525 G u Thr Al a G u G n 605 Phe Ser Asp Sy 335 G y Arg Al a Asp Trp n Asp y 400 Asp Asp 415 G y G u Pr o Al a G u Leu Asn Phe 480 Thr G u 495 Leu G y let G u Phe Asp Ser G y 560 Leu Arg 575 I e y G y Lys G u Hi s Ser Val 640 Val G y 655 Thr G y Ser Ser Thr Ala G u Thr Val Ser Trp lie Ser Leu Phe Thr Ala Leu Page 596 Gene Sequence 24-2-06.txt 675 680 685 Ser Ser Leu Val Phe Thr Leu Ala Leu Asn n G u Ala Leu G n Asn 690 695 700 Leu Ile Asn G n Phe ebt Arg G n Phe Lys 705 710 <210> 465 <211> 389 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(382) <223> RXN03015 <400> 465 ccgggtttgc ctgaggtttt ggagcgcaat ctggaaaatg gtcgcctgaa cttcacta gattatgcgg aggctgcggc tttcgcgcag gtgcatttct ttg ggc gtg ggt act ct cct Pr o gtt Val ggc Gy gct Al a ccg Pr o gac Asp cag G n gag G u tct Ser tct Ser ttc Phe at c SIle ggt Gy ttg Leu gtt Val gt c Val cgt Ar g gt g Val t at Tyr ccg Pr o gtt Val cct Pr o ggc Gy gt g Val gcg Al a ct g Leu ggt Gy 45 ggt Gy t ac Tyr cgt Ar g gcg Al a ctt Leu act Thr tcg Ser gcg Al a gaa G u gat ctg Asp Leu 15 gag ggc G u Gy gct gag Al a G u cac gtg His Val gtc aaa Val Lys 80 taagaca Leu Gy Val Gy Thr 1 acg tat gtt cgt cag Thr Tyr Val Arg G n gag cac att att ttc G u His Ile Ile Phe cag ttg cag gag ctt G n Leu G n G u Leu gag atc gcg tgg aat Su lie Ala Trp Asn gac acc atc act ccg Asp Thr Ile Thr Pro <210> 466 <211> 94 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 466 Leu y Val Oy Thr Pro n n Lys Oy Thr Tyr Ala Ala Asp Leu 1 5 10 Thr Tyr Val Arg G n Val Val G u Asp Leu Val Pro Leu Leu G u y 25 Su His Ile Ile Phe y Lys Ser Thr Val Pro Val y Thr Ala u 40 G n Leu G n G u Leu Ala Asp Ser Leu Val Lys Pro G y Ser His Val 55 Page 597 ene Sequence 24- 2- 06. t xt Ou IlieAla Trp Asn Pro Ou Phe Leu Arg Ou Gy Tyr Ala Val Lys 70 75 As p Thr I Ie Thr Pro Asp Arg lieVal Val Gy ValI Ar g O31u <210O> 467 <21 1> 801 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (778) <223> RXN03056 <400> 467 t tact at cac t ggtt t tgca gat gagat cg cacat gat ct ttaataaact gaagaacagc tcgcggaagc taaagccaaa cgacgaacag at t tcct tac ttg gat gcc gca Leu Asp Al a Al a at t I Ie g at As p gct Al a gca Al a ac c Th r c ac Hi s g at As p gca Al a ccg Pr o at t I Ie 1 50 ggC (31y ct t Le u tt t Ph e ct c Le u ggC G31y gca Al a aat As n at c I Ie aac As n 120 gt t ValI g at As p ggC (31y gcc Al a g at As p ggC G31y aac As n gt c ValI aag Ly s ag c Ser gt g ValI g ac As p gag (31u t ac Tyr 1 70 gt t ValI cac Hi s gcg Al a cct Pr o gaa (31u 75 gga G31y at c I Ie caa O31n t ac Tyr cac Hi s 155 cca Pr o gga G31y t tg Le u aaa Ly s g aa (31u ct c Le u at c I Ie g ac As p ac c Th r gt c ValI 140 ccc Pr a gag (31u t cc Ser gag (31u t ac Tyr 45 ag c Ser gca Al a t at Tyr t cc Ser gga G31y 125 g ac As p at c I Ie ct c Le u g ac As p cgc Ar g at c I Ie t tc Ph e g aa (31u G31y cct Pr a 110 t tc Ph e t ac Tyr gga G31y ct t Le u t tc Ph e 15 gca Al a cgc Ar g cgc Ar g gcc Al a g ac As p 95 aac As n aag Ly s gt c ValI at c I Ie gcc Al a 1 75 ggc G31y cgc Ar g at g vb t aaa Ly s ggc G31y 80 t cc Ser t ac Tyr cct Pr a c ac Hi s at c I Ie 1 60 gcg Al a at c I Ie ggt G31y t cc Ser gt a ValI at c I Ie c ag O31n gca Al a g at As p 130 aag Ly s cca Pr a aac As n aac As n gt t ValI t tc Ph e ct c Le u ac c Th r cgc Ar g at c I Ie 115 gag O31u g ac As p gca Al a gcc Al a at c lIe 20 gag O31u tt t Ph e t cc Ser ct c Le u gt g ValI 1 00 t ac Tyr gca Al a gcg Al a gga G31y g ac As p 1 gga G31y ac c Th r gt c ValI at t I Ie cgc Ar g ct c Le u aag Ly s g ac As p t gg Tr p ac c Th r c aa O31n 1 ggt (31y Page 598 Gene Sequence 24- 2- 06. txt tac aac gga ttc gtc tcc atc gag cct cac ctg ggt gac ttc gat gaa 691 Tyr Asn G y Phe Val Ser lie G u Pro His Leu G y Asp Phe Asp G u 185 190 195 ttc ggc gga ctc tgc gga cct gac ctg tgg acc agc gca tgc gac gct 739 Phe y G y Leu Cys G y Pro Asp Leu Trp Thr Ser Ala Cys Asp Ala 200 205 210 ctc gca gga atc ctg aac aac atc aac gcc gag tac aac taaggacaac 788 Leu Ala G y Ile Leu Asn Asn Ile Asn Ala G u Tyr Asn 215 220 225 tgataatgac aaa 801 <210> 468 <211> 226 <212> PRT <213> Corynebact eri um gl ut am cum <400> 468 Leu Asp Ala Ala Gy I le Ser Leu Ser Ala Val y Ser Asp Phe y 1 5 10 Lys I e Asn I e Thr Asp Pro Phe Gu Asp His Leu u Arg Ala Arg 25 Hi s G y Val G u Val Ala Lys Leu Phe G y Ala Lys Tyr II e Arg IVet 40 Phe Ser Phe Phe lie Al a G u y Asp Asn Pro u Ser Phe Arg Lys 55 G u Val Leu Ser Arg Thr Hi s Al a et Val G u Leu Al a G u Al a G y 70 75 G y IIe Thr Leu Leu His G u Asn G u Lys G y I I e Tyr G y Asp Ser 90 Pro G n Arg Val Lys Asp Leu Ile Thr Ser Ile Asp Ser Pro Asn Tyr 100 105 110 Arg Ala lie Tyr Asp Al a Ala Asn Tyr Val G n Thr y Phe Lys Pro 115 120 125 Phe Asp G u Ala Trp Pro Ile Val Lys Asp Tyr Val Asp Tyr Val His 130 135 140 IIe Lys Asp Ala Thr IIe Pro Asp Al a u His Pro lie G y lie lie 145 150 155 160 Lys Pro Ala Gy On Gy Asp Gy n Tyr Pro u Leu Leu Ala Ala 165 170 175 Leu Asn Ala Asp G y Tyr Asn G y Phe Val Ser lie G u Pro His Leu 180 185 190 G y Asp Phe Asp G u Phe G y G y Leu Cys G y Pro Asp Leu Trp Thr 195 200 205 Ser Al a Cys Asp Al a Leu Al a G y Ile Leu Asn Asn Ile Asn Al a G u 210 215 220 Tyr Asn 225 Page 599 Gene Sequence 24-2-06.txt <210> 469 <211> 687 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(664) <223> RXN03030 <400> 469 gaagatgaag cagaaaagat cattggtgcg ccagaggttt cagcttgatt ccgtcacctt gctgcgtaac aaccccatcc ctgcattggg caacaaagca cct Pr o at c l Ie cca Pr o gt g Val gtt Val gtt Val acc Thr gt g Val ggt Sy t cc Ser 150 ct c Leu t ac Tyr gca Al a gag G u gt a Val gct Al a ct c Leu gct Al a cct Pr o act Thr 120 gag G u cag G n gag G u cac Hi s ct g Leu caa G n ttg Leu cct Pr o gtt Val gaa G u gt g Val gag G u gga G y at t SIle t ac Tyr 170 aag Lys ct a Leu gt g Val gaa G u gac Asp 75 gct Al a ct g Leu at c SIle cca Pr o gct Al a 155 acc Thr at c SIle cag G n tct Ser at t I e cct Pr o gca Al a tct Ser aag Lys aag Lys 140 gat Asp t ac Tyr t ac Tyr ct g Leu t cc Ser 45 gca Al a cgt Ar g gt c Val gag G u cca Pr o 125 ggc G y t cc Ser gt g Val cca Pr o gaa G u gag G u ct g Leu gcc Al a cca Pr o at c SIle 110 gag G u aag Lys cct Pr o gac Asp ttg Leu 15 gca Al a t ca Ser ttt Phe aat Asn acc Thr 95 gag G u ttc Phe ct g Leu cgc Ar g t cc Ser 175 gtt Val gcc Al a gaa G u gaa G u ggt Gy at c SIle cct Pr o ctt Leu cca Pr o gat Asp 160 gca Al a gt g Val 1 act Thr at t l Ie gca Al a gat Asp gt c Val ttg Leu ggt Gy ct c Leu ttg Leu 145 at c SIle ggg G y ggc G y cgc Ar g gat Asp gac Asp gat Asp gct Al a gcc Al a aag Lys 130 act Thr cca Pr o at g let cgt Ar g gca Al a ctt Leu ct c Leu gt g Val gt g Val gct Al a 115 gct Al a gtt Val ggc G y gcc Al a acc Thr gaa G u gca Al a gaa G u gaa G u aac Asn 100 gcc Al a ttg Leu cct Pr o aag Lys t ac Tyr 180 ct g cca ct g Leu Pro Leu ctt aat ttc Leu Asn Phe tagattgtag gtagtctcgt ggg <210> 470 <211> 188 Page 600 Gene Sequence 24-2-06.txt <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 470 Val Leu Pro 1 Thr SIle Al a Asp Val Leu Gy Leu Leu 145 I le Gy Ar g Al a Leu Leu Val Val Al a 115 Al a Val Gy Al a Leu Asp 5 Thr Lys G u Leu Ala I e G u G y G u Ar g Asn Phe 100 Al a Val Leu Thr Pro Al a Lys Phe 165 Tyr Lys 180 Asp Gy Tr p Ser G n Asn Gy G n 135 bet Asp Gy G u Val 40 Al a Leu Al a Pr o Thr 120 G u G n G u Hi s Pro Ala Al a Ser Val Thr Ar g Ser Val Tr p 105 Phe Gy Al a Ser Gy 185 Lys Leu Val G u Asp Al a Leu SIle Pr o Al a 155 Thr Asn SIle G n Ser I e Pr o Al a Ser Lys Lys 140 Asp Tyr Phe Tyr Leu Ser Al a Ar g Val G u Pr o 125 Sy Ser Val Leu Al a Ser Phe Asn Thr G u Phe Leu Ar g Ser 175 <210> 471 <211> 1062 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1039) <223> RXN00401 <400> 471 cttttaagca tttcaacatg ccaaactaat cttggacagg tatcactggc tcctcttaga ctccaaagtg tcttagaaaa cactcaccca aggagccctc atg gca cgc ttt tca Met Ala Arg Phe Ser 1 cca caa gat ctc gca gac cac ctc aag gat gga ctg ctc tct ttc ccg Pro G n Asp Leu Ala Asp His Leu Lys Asp G y Leu Leu Ser Phe Pro 15 gcc acc gct ttc caa gat gac ctc gaa gta gat gaa gct gct tat gtc Ala Thr Ala Phe G n Asp Asp Leu G u Val Asp G u Ala Ala Tyr Val 30 gag cac att gaa tgg cag tcc agc tac cca gtc gcc ggc ctc ttc gca G u His lie G u Trp G n Ser Ser Tyr Pro Val Ala G y Leu Phe Ala Page 601 Gene Sequence 24- 2- 06. txt 45 gct ggc ggt act ggc gaa gga ttc agc ctt acc gtt gag gaa aac cac 307 Ala G y G y Thr G y G u G y Phe Ser Leu Thr Val G u G u Asn His 60 cgt gtc act caa ctt gca gtt cag gcg tcc agc ccg gaa gtt ccc gtg 355 Arg Val Thr G n Leu Al a Val G n Ala Ser Ser Pro G u Val Pro Val 75 80 ttg ggg tct gct act ggc tca act aag tct gcc atc gca aac gca cag 403 Leu y Ser Ala Thr G y Ser Thr Lys Ser Ala Ile Ala Asn Ala G n 95 100 ggc gca gag gca gca ggc gct gaa ggt gtc ctc ctg ctt cct ccc tac 451 Gy Ala G u Ala Ala G y Ala G u y Val Leu Leu Leu Pro Pro Tyr 105 110 115 ctc acc gaa tgc gac gca gaa ggc ctg tac aac cat gca gcc gca gtc 499 Leu Thr G u Cys Asp Al a G u G y Leu Tyr Asn Hi s Al a Al a Al a Val 120 125 130 tgt gaa tcc act tct ctt ggt gtc atc gtg tac aac cgt gcc aat gcc 547 Cys u Ser Thr Ser Leu y Val lie Val Tyr Asn Arg Ala Asn Ala 135 140 145 atc tac tcc cca gag gtt atc gct cga ctc tct gag cgc tac ccc aac 595 Ile Tyr Ser Pro G u Val Ile Ala Arg Leu Ser G u Arg Tyr Pro Asn 150 155 160 165 ttc att gga ttt aaa gat ggc acc gga aac atc gag cac cta gca aag 643 Phe IIe G y Phe Lys Asp G y Thr G y Asn lie G u Hi s Leu Al a Lys 170 175 180 atc acc acg cta tgc gga gat cgc ctg ttc tac ctc ggt gga ctt ccc 691 Ile Thr Thr Leu Cys G y Asp Arg Leu Phe Tyr Leu Gy y Leu Pro 185 190 195 acc gct gag acc ttt gca cta cca ctg ctt cag atg ggc atg agc acc 739 Thr Ala G u Thr Phe Al a Leu Pro Leu Leu G n IVet y IVet Ser Thr 200 205 210 tac tcc tct gca atg ttc aac ttc att cca gat ttc gca ctg agc ttc 787 Tyr Ser Ser Ala IVet Phe Asn Phe lie Pro Asp Phe Ala Leu Ser Phe 215 220 225 tac gcc gat gtt cgt gcg cag gac agc gca gca gta aag cag aag ctg 835 Tyr Ala Asp Val Arg Al a G n Asp Ser Ala Ala Val Lys G n Lys Leu 230 235 240 245 agc gat ttt gtg ctc ccc tac ttg gat atc cgc gat cgc gca caa ggc 883 Ser Asp Phe Val Leu Pro Tyr Leu Asp lie Arg Asp Arg Ala G n y 250 255 260 tac ggt gtc tcc att ggt aag ggc gga ctc aag gct gtt ggc cgc aac 931 Tyr y Val Ser Ile y Lys G y y Leu Lys Ala Val y Arg Asn 265 270 275 gct ggc ggc gtt cgc cca cca ctg cgt aac ctt tcc gag caa gat atc 979 Ala y y Val Arg Pro Pro Leu Arg Asn Leu Ser G u G n Asp Ile 280 285 290 gcg gac ctg tcg gat ttg ctt gcc acc tct ggc gca ggt tcc tac cgc 1027 Ala Asp Leu Ser Asp Leu Leu Ala Thr Ser y Ala y Ser Tyr Arg 295 300 305 ctt cag ttg agg tgaaagcatg atcaccgcaa ccg 1062 Leu G n Leu Arg Page 602 Gene Sequence 24-2-06.txt 310 <210> 472 <211> 313 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 472 MVt Ala A 1 Leu Leu S G u Al a A Al a y L Val Gu G Pro Gu V I e Al a A Leu Leu P 1 Hi s Al a A 130 Asn Arg A 145 G u Arg T G u Hi s L Leu G y G 1 Mvt y M 210 Phe Al a L 225 Val Lys G Asp Arg A Ala Val G 2 Ser G u G 290 Al a G y S 305 rg Phe er Phe I a Tyr eu Phe lu Asn al Pro sn Al a 100 ro Pro 15 I a Al a I a Asn yr Pro eu Al a 180 Sy Leu 95 tt Ser eu Ser In Lys I a n 260 ly Arg 75 In Asp er Tvr Ser 5 Pr o Val Al a Hi s Val G n Tyr Val Al a Asn 165 Lys Pr o Thr Phe Leu 245 Sy Asn SIle Ar g Pro G n Asp Leu Al a G u Al a Ar g 70 Leu Sy Leu Cys l Ie 150 Phe SIle Thr Ty r Ty r 230 Ser Ty r Al a Al a Leu 310 Thr Hi s Sy Val Sy Al a Thr G u 135 Tyr SIle Thr Al a Ser 215 Al a Asp Sy Sy Asp 295 G n Al a SIle 40 Sy Thr Ser G u G u 120 Ser Ser Sy Thr G u 200 Ser Asp Phe Val Sy 280 Leu Leu Phe G u Thr G n Al a Al a 105 Cys Thr Pr o Phe Leu 185 Thr Al a Val Val Ser 265 Val Ser Ar g Al a 10 G n Tr p Sy Leu Thr 90 Al a Asp Ser G u Lys 170 Cys Phe bet Ar g Leu 250 I le Ar g Asp Asp Asp G n G u Al a 75 Sy Sy Al a Leu Val 155 Asp Sy Al a Phe Al a 235 Pr o Sy Pr o Leu Leu Leu Ser Phe G n Thr G u Sy 125 Val Al a Thr Ar g Pr o 205 Phe Asp Leu Sy Leu 285 Al a Asp Val Pr o Leu Ser Ser Val Tyr Val Leu Asn 175 Phe Leu Pr o Al a SIle 255 Leu Asn Ser Sy Asp Val Thr Ser Al a Leu Asn Tyr Ser 160 I e Tyr G n Asp Al a 240 Ar g Lys Leu Sy Page 603 Gene Sequence 24-2-06.txt <210> 473 <211> 924 <212> DNA <213> Corynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(901) <223> RXN02125 <400> 473 gacctaaaat tcatcacctc accgttttta aggcttagaa aaatagcagt gttgggatgt atg gtg gca acc tct 11! let Val Ala Thr Ser gaatatccat ttatgctgct gtagtcggct atgtggacgc cag G n gtt Val cgc Ar g ct c Leu gca Al a gt c Val ggt Sy gat Asp ct g Leu cac Hi s 150 at c l Ie at c SIle at c SIle gga Gy gtt Val at t SIle gcc Al a ct g Leu gac Asp t ac Tyr 120 gcc Al a aat Asn ttc Phe cac Hi s gac Asp cga Ar g gaa G u acc Thr at t l Ie ct c Leu t ca Ser gcc Al a ct g Leu ctt Leu gat Asp 170 gag G u agc Ser aac Asn ggc G y aag Lys gat Asp 75 at t SIle gag G u gga Gy gag G u ctt Leu 155 t cc Ser at g bet gag G u at t lile at t SIle ct a Leu gag G u cac Hi s gga G y aag Lys aat Asn 140 gt g Val aag Lys ct g Leu gct Al a gat Asp cgc Ar g 45 gct Al a tct Ser t cc Ser aac Asn at t SIle 125 at c SIle cat Hi s ggc Gy aag Lys gcc Al a act Thr acc Thr gca Al a ttg Leu cca Pr o cgt Ar g 110 cga Ar g tta Leu gtt Val at t SIle aac Asn 190 cag G n 15 gcc Al a gct Al a gaa G u gcg Al a caa G n 95 gaa G u t cc Ser gac Asp gga Gy ct g Leu 175 cag G n gcg Al a ct a Leu gga G y at c SIle aaa Lys cca Pr o gcg Al a at t SIle t cc Ser aac Asn 160 gt c Val cag G n 1 gt a Val gcg Al a gt g Val aaa Lys at t SIle tgg Tr p tgg Tr p ggt G y gcg Al a 145 acc Thr gaa G u gt c Val cgc Ar g t at Tyr ccc Pr o gat Asp ggc G y agt Ser cgc Ar g gt c Val 130 acg Thr cca Pr o gca Al a aag Lys gca Al a gga G y cgc Ar g t ac Tyr ttg Leu gat Asp gcg Al a 115 tcg Ser gtt Val agc Ser t at Tyr gcg Al a 195 gct Al a aac Asn gag G u gat Asp gat Asp ttc Phe 100 ctg Leu aac Asn gct Al a gag G u t ca Ser 180 att SIe gac aag tac aac gtg agc att ccg cag cta tgc att cgg tac aca att Page 604 Asp caa G n agc Ser 230 gca Al a cct Pr o Tyr 200 gga G y aat Asn caa G n t at Tyr Val gt g Val cag G n gt g Val 250 ggc Gy Gene Sequence 24-2-06.txt lie Pro n Leu Cys lie Arg Tyr Thr Ile 205 210 ttg cca aag act gcc aac cca gat cat atg Leu Pro Lys Thr Ala Asn Pro Asp His IVet 220 225 gac ttt gaa att tcc gag gaa gac atg gcg Asp Phe G u Ile Ser G u G u Asp IVet Ala 240 245 gcc cgc gat tat ggc gag cac agc ggt ttt Ala Arg Asp Tyr G y G u His Ser G y Phe 255 260 tagaaagatt tttatcatgg gac <210> 474 <211> 267 <212> PRT <213> Corynebacteri um gl ut arri cum <400> 474 Net Val Ala 1 Val Arg Ala Al a Tyr G y Val Pro Ar g Lys Asp Tyr I e G y Leu Trp Ser Asp Tr p Ar g Al a 115 G y Val Ser 130 Al a Thr Val 145 Thr Pro Ser G u Al a Tyr Val Lys Al a 195 I e Arg Tyr 210 Thr Ser 5 Ala I e Asn u G u G u Asp y Asp Tyr Phe Arg 100 Leu G u Asn Phe Ala Pro G u Leu 165 Ser Pro 180 l e Al a Thr Ile Phe Al a Sy Phe Val Asp Sy Al a G u 135 Val Ser Al a Lys Leu 215 Asp Tyr 25 Sy Ser Al a Net Tyr 105 Lys Asp G n Cys Gy 185 Asn Thr Asp 10 Ar g G u Thr l Ie Leu 90 Ser Al a Leu Leu Asp 170 G u Val Ser Asn Gy Lys Asp I le G u Gy G u Leu 155 Ser bet Ser Al a Asp Ar g Al a Ser Ser Asn SIle 125 I le Hi s Gy Lys Pr o 205 Al a Thr Thr Al a Leu Pr o Ar g 110 Ar g Leu Val SIle Asn 190 G n G n Al a Al a G u Al a G n G u Ser Asp Sy Leu 175 G n Leu Val Ser Leu Pro Lys Thr Ala 220 Page 605 Gene Sequence 24-2-06.txt Asn Pro Asp His IVet Ser Ser Asn Ala Gn lie Asp Phe Ou lie Ser 225 230 235 240 G u u Asp Iet Ala Ala Leu Gn u Val Thr Ala Arg Asp Tyr y 245 250 255 G u His Ser G y Phe Pro Val Tyr Ser G y Lys 260 265 <210> 475 <211> 3561 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(3538) <223> RXN00200 <400> 475 aacccggagt catttgatca gatgtggcag accaagcaag acgcacaatg agtaattcct caccaaacga cccaagccct tgggacct t t cgtggtggta ggt Sy gag Su ccg Pr o ttg Leu tcg Ser ct g Leu gcg Al a cct Pr o at c SIle ctt Leu 150 ggg G y aag Lys tgg Tr p ttt Phe t ca Ser t cc Ser gac Asp gac Asp 120 ggt Gy cca Pr o cat Hi s ct a Leu aag Lys ct g Leu cca Pr o gca Al a ct c Leu acg Thr ctt Leu gag G u caa G n aag Lys aaa Lys tt g Leu caa G n 75 ccg Pr o aat Asn gac Asp gac Asp gt g Val 155 ct a Leu ggt G y ct g Leu cct Pr o aat Asn cag G n gac Asp gcc Al a gt g Val 140 aac Asn gac Asp tct Ser gct Al a 45 ttc Phe ggt G y tcg Ser aat Asn acc Thr 125 at t SIle cgt Ar g tcg Ser gcg Al a at c l Ie ct g Leu gag G u at g bet cag G n 110 aac Asn gtt Val ct g Leu at c I e 15 caa G n t ca Ser cca Pr o ctt Leu gat Asp 95 tcg Ser cgt Ar g cgc Ar g cca Pr o aac Asn gaa G u t ca Ser gt g Val t cc Ser 80 gcg Al a ttg Leu ggt G y ggt G y gat Asp 160 cgc caa gtc ggt Arg n Val y tca gat gta gtt Ser Asp Val Val ccg cag gtt gcc Pro G n Val Ala ctg ctt ggc ctg Leu Leu G y Leu cag gtg cag tct G n Val G n Ser gtt aac gcc ccg Val Asn Ala Pro atc cct gtg tcc Ile Pro Val Ser 100 atg ggc acg ttg IVet y Thr Leu 115 ttt gtg cgc acc Phe Val Arg Thr 130 gtg ctg ttg gat Val Leu Leu Asp gcg atc cta gag Ala lle Leu G u 165 att tcc tcc acc gag gaa acc acc agc gcg gaa atc acc ggc acg gca Page 606 lie Ser Ser Thr G u G u Thr 170 Gene Sequence 24-2-06. t xt Thr Ser Al a u Ile Thr y Thr Ala ttc Phe t ac Tyr gcg Al a ccc Pr o 230 ttg Leu aaa Lys gat Asp aac Asn aac Asn 310 t ca Ser at c SIle ct g Leu t ca Ser ttc Phe 390 at c SIle at c SIle ggc G y gag G u 200 tta Leu ctt Leu t cc Ser cac Hi s gtt Val 280 tct Ser gat Asp ttc Phe acc Thr at g Net 360 gtt Val gcg Al a gcg Al a acc Thr acc Thr gt c Val gtt Val aag Lys tgg Tr p 250 ttc Phe gt a Val gac Asp at g Met gca Al a 330 t ca Ser ttc Phe ggt G y tgg Tr p gga G y 410 agg Ar g gaa G u gac Asp gat Asp tac Ty r 235 aca Thr ct g Leu gcg Al a ggc G y gcc Al a 315 cca Pr o at c SIle gt g Val cgc Ar g ctt Leu 395 gct Al a tt g Leu ggc G y gac Asp at t SIle 220 gca Al a ct g Leu cct Pr o at t l Ie ttc Phe 300 aac Asn t at Tyr tgg Tr p at c SIle cgc Ar g 380 cca Pr o ct a Leu ttg Leu gat Asp ccc Pr o 205 gag G u gcc Al a cac Hi s gcc Al a ttg Leu 285 at c SIle t ac Tyr t ac Tyr ctt Leu acc Thr 365 gtt Val t ac Tyr ctt Leu ccc Pr o gag G u 190 t cc Ser at c SIle at c l Ie cgc Ar g aac Asn 270 gt g Val at g et t ac Tyr gac Asp cgt Ar g 350 aga Ar g gcg Al a aac Asn gcg Al a gct Al a 430 175 cgg Ar g acc Thr aac Asn ttc Phe at g Net 255 tgg Tr p ttc Phe acc Thr cgc Ar g ttg Leu 335 ct a Leu gag G u cac Hi s aac Asn tgg Tr p 415 gcc Al a cct Pr o gca Al a t cc Ser at t SIle 240 gat Asp t ac Tyr tgg Tr p at g Net tgg Tr p 320 ct g Leu ccc Pr o gt c Val tgg Tr p ggc G y 400 gtt Val at t l Ie cag G n tcg Ser cgc Ar g 225 ggc G y at t SIle aag Lys cac Hi s gcc Al a 305 ttc Phe gct Al a gca Al a at g Net t ct Ser 385 act Thr t cc Ser ggt G y gt c Val gcc Al a 210 ttc Phe ctt Leu ttg Leu ct g Leu ttc Phe 290 cgc Ar g ggt G y ct g Leu ttg Leu cca Pr o 370 gca Al a cgc Ar g ttc Phe gt c Val acc Thr 195 ct g Leu act Thr gcg Al a gat Asp aag Lys 275 ctt Leu gt g Val gt c Val at g Net ct c Leu 355 cgg Ar g gcc Al a cca Pr o gag G u at t SIle 435 180 ggc G y gcc Al a t ca Ser tct Ser ggt G y 260 cca Pr o ggc G y t cc Ser cca Pr o gcc Al a 340 gct Al a ttt Phe at g Net gag G u cgc Ar g 420 at c SIle gtt Val t ca Ser t cc Ser gt g Val 245 cga Ar g ctt Leu gcc Al a cag G n gaa G u 325 t ac Tyr gga G y ggc G y gt g Val cca Pr o 405 gct Al a gcc Al a 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 acc att tcc ctg gca tca ggc ccc acc ggt cta atg gca gtt gct gcg Page 607 Thr Ile Ser Leu Ala Ser Gy 440 Gene Sequence 24-2-06.txt Pro Thr y Leu Met Ala Val Ala Ala ttg Leu ccg Pr o 470 gct Al a ct c Leu at c SIle t at Tyr acc Thr 550 gt c Val cca Pr o at g et ctt Leu gcg Al a 630 ttc Phe t cc Ser at c SIle at c SIle gt c Val at t SIle at g Net gcc Al a gt g Val 520 cgc Ar g cgt Ar g gcg Al a ct g Leu acc Thr 600 ggc G y aaa Lys ct g Leu t ac Tyr gca Al a 680 gtt Val ttg Leu gcg Al a at g Net 490 ttt Phe t cc Ser caa G n gct Al a ct g Leu 570 ttg Leu acc Thr ttg Leu cca Pr o ct g Leu 650 gt g Val acc Thr caa G n t cc Ser tcg Ser 475 ctt Leu ggc G y gcg Al a acc Thr gt g Val 555 cgt Ar g at g bet aag Lys gcc Al a cgc Ar g 635 gct Al a cca Pr o gt a Val t cc Ser gcg Al a 460 agg Ar g gcc Al a gat Asp aag Lys gt c Val 540 ct g Leu t ac Tyr at g Net tgg Tr p gga G y 620 at g Net ct c Leu tgg Tr p gt g Val ttt Phe 700 445 ttg Leu ggg Gy cca Pr o cag G n ggc Gy 525 at g Net at g bet ggc G y gt c Val act Thr 605 ctt Leu cgc Ar g gca Al a tgg Tr p ct c Leu 685 gt c Val at t SIle gcg Al a ttc Phe act Thr 510 ccg Pr o gaa G u ct c Leu cgc Ar g at t SI e 590 cac Hi s gct Al a acc Thr ggc Gy gat Asp 670 gt g Val cac Hi s cgc Ar g tcg Ser ctt Leu 495 ct g Leu gca Al a caa G n at g Net at t SIle 575 ttc Phe cac Hi s gcc Al a at t SIle gt g Val 655 aaa Lys at c SIle gat Asp at t SIle aaa Lys 480 gcg Al a t ca Ser ct g Leu acc Thr gcg Al a 560 cca Pr o G y ttc Phe gt g Val t cc Ser 640 aac Asn acc Thr gcc Al a gt g Val ctt Leu 465 agc Ser tct Ser acc Thr acc Thr gtt Val 545 tgt Cys ggc G y acc Thr ggt G y ggg G y 625 at c SIle gga G y at c SIle gt g Val aaa Lys 705 450 t at Tyr aaa Lys ggc G y gt c Val tgg Tr p 530 gat Asp ct g Leu gct Al a at g bet gt c Val 610 ct g Leu ggt G y ttc Phe cag G n at c SIle 690 acc Thr agg Ar g gt c Val acc Thr at g Net 515 t ac Tyr ggt G y gct Al a gcg Al a ttc Phe 595 t ac Tyr t cc Ser gcg Al a tgg Tr p at c SIle 675 gt g Val gcg Al a cgc Ar g ttt Phe gcg Al a 500 gaa G u cac Hi s t cc Ser at t l Ie aag Lys 580 ttc Phe gca Al a t at Tyr ttc Phe t ac Tyr 660 aag Lys ct g Leu caa G n ttg Leu ggc G y 485 at t SIe t cc Ser gaa G u ttc Phe gt g Val 565 gga G y at g bet gga G y gtt Val ct c Leu 645 acc Thr ggc G y at c SIe gcc Al a 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 2275 gaa acc aat cac tcc atg ggc gaa ctc gtg gcg gaa gat gaa gca aag Page 608 Su Thr Asn His Ser MVt y 710 715 Gene Sequence 24-2-06.txt G u Leu Val Ala G u Asp G u Ala cgc Ar g gga Gy ggc Gy cgc Ar g gaa G u 790 ggc Gy at c SIle gcc Al a t ca Ser ggc G y 870 caa G n aac Asn ccc Pr o aac Asn ctt Leu 950 gac Asp cgt Ar g t cc Ser gt g Val 760 ct g Leu aac Asn t cc Ser cca Pr o gcc Al a 840 gga G y aac Asn cca Pr o acc Thr at c SIle 920 gt c Val gat Asp ggc Gy t cc Ser 730 ct c Leu caa G n ggc Gy aac Asn gaa G u 810 at c SIle act Thr t cc Ser t cc Ser gt c Val 890 gac Asp gt a Val caa G n ggc Gy gag G0u 970 agg Ar g gtt Val tac Ty r aac Asn gat Asp 795 t cc Ser agc Ser gac Asp acc Thr aac Asn 875 ggc G y tgg Tr p tct Ser t cc Ser gac Asp 955 cca Pr o ttc Phe gt g Val ccc Pr o aca Thr 780 t cc Ser gaa G u cag G n gac Asp ggc G y 860 gcc Al a t cc Ser t ac Tyr gca Al a gga Gy 940 gtt Val t cc Ser acc Thr ct g Leu gcg Al a 765 tgt Cys ttc Phe gat Asp gac Asp t cc Ser 845 aac Asn cgc Ar g tgg Tr p gaa G u gca Al a 925 caa G n gaa G u tgg Tr p ggc G y at t SIle 750 t ac Tyr ggc Gy ct c Leu at t SIle caa G n 830 acc Thr acc Thr ct g Leu t cc Ser at c SIle 910 ggt G y t cc Ser gac Asp cgc Ar g ctt Leu 735 acc Thr t cc Ser ctt Leu act Thr cgc Ar g 815 gca Al a gaa G u ggc G y cca Pr o gct Al a 895 cca Pr o cgc Ar g gt c Val ct c Leu aac Asn 975 720 gcg Al a tgc Cys gt g Val gcc Al a cca Pr o 800 ggc G y gac Asp acc Thr ggt G y ttc Phe 880 ggc G y gaa G u at c SIle at g let ggc G y 960 ct c Leu gcc Al a gca Al a ggt G y t cc Ser 785 gt g Val ttt Phe ct g Leu ggc G y gt c Val 865 aac Asn acc Thr gcc Al a gaa G u ct c Leu 945 gaa G u cgc Ar g t cc Ser t cc Ser ctt Leu 770 gac Asp aac Asn agt Ser tct Ser gga G y 850 cga Ar g ct g Leu caa G n acc Thr cac Hi s 930 gaa G u gcc Al a t ac Tyr cct Pr o at g let 755 ggc G y gcc Al a t cc Ser gct Al a gct Al a 835 t cc Ser ggc G y gac Asp aac Asn gaa G u 915 t ac Tyr t ac Tyr at g bet cca Pr o at c SIle 740 ggc G y aac Asn at g Net aca Thr gcc Al a 820 gtt Val gac Asp t cc Ser t ac Tyr cca Pr o 900 gaa G u gac Asp ggt G y at g bet ctt Leu 980 gca Al a aaa Lys ct c Leu ct g Leu ctt Leu 805 ggc ggt G y gaa G u gaa G u acc Thr 885 gca Al a gca Al a at c SIe cgc Ar g t ac Tyr 965 gac Asp 2323 2371 2419 2467 2515 2563 2611 2659 2707 2755 2803 2851 2899 2947 2995 3043 3091 caa ctc cca gaa gaa gcg gac gtc gtg cgc atc gtc gcc acc gac gtc Page 609 Gene Sequence 24- 2- 06. txt Sn Leu Pro u u Ala Asp Val Val Arg Ile Val Ala Thr Asp Val 985 990 995 aac ctc gac gaa gac caa tgg gta gca ctg acg cca cca cgc gta cct 3139 Asn Leu Asp G u Asp G n Trp Val Ala Leu Thr Pro Pro Arg Val Pro 1000 1005 1010 aac cta gat tct ctg aac aac gtc atc gga tcc gaa acc cca gga ctc 3187 Asn Leu Asp Ser Leu Asn Asn Val I e y Ser G u Thr Pro y Leu 1015 1020 1025 ctc gac tgg gca gtt ggc ctg caa ttc cca tgc caa cgc acc ttc gac 3235 Leu Asp Trp Ala Val G y Leu G n Phe Pro Cys G n Arg Thr Phe Asp 1030 1035 1040 1045 cac tac gcc gga gtc acc gag att cct gaa tac cga atc tcc cca gac 3283 His Tyr Ala G y Val Thr G u lie Pro G u Tyr Arg lie Ser Pro Asp 1050 1055 1060 cac ggc gga aaa tcc acc ctc tcc cca ttc caa gac tgg gca ggc ggc 3331 His G y G y Lys Ser Thr Leu Ser Pro Phe G n Asp Trp Ala G y G y 1065 1070 1075 gga tcc atg ggc acg gcc gaa gca gta aac aac gcc tac gaa atc ccg 3379 G y Ser IVet G y Thr Al a u Al a Val Asn Asn Ala Tyr G u Ile Pro 1080 1085 1090 tcc tac ctc cgc aac gac tgg ggc cgc gac tgg ggt tcc atc gaa cgc 3427 Ser Tyr Leu Arg Asn Asp Trp y Arg Asp Trp G y Ser lie G u Arg 1095 1100 1105 tac tcg ctg cgc acc aac tcc aac ggc gat gca cct aag gtt gca gac 3475 Tyr Ser Leu Arg Thr Asn Ser Asn y Asp Ala Pro Lys Val Ala Asp 1110 1115 1120 1125 atc aac ctt gaa acc atc caa cgt tcc gga ctc tgg aat cca ggg cat 3523 lie Asn Leu G u Thr lie G n Arg Ser G y Leu Trp Asn Pro Gy His 1130 1135 1140 atg aag gta gat gaa taagaccttc agtactggaa gtt 3561 NIet Lys Val Asp G u 1145 <210> 476 <211> 1146 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 476 IVet Arg G n Val G y G y Asn G y G y His n Leu Asp Ser lie Asn 1 5 10 Val Ser Asp Val Val u Ser Lys Lys Leu Lys y Ser Ala n G u 25 Pro Pro G n Val Ala Pro y Trp Leu Lys Lys Leu Ala lie Ser Ser 40 G y Leu Leu G y Leu Leu IVet Phe Val Leu Leu Pro Phe Leu Pro Val 55 Asn G n Val G n Ser Ser Leu Ser Trp Pro G n Asn y G u Leu Ser 70 75 Ser Val Asn Ala Pro Leu Ile Ser Tyr Ala Pro G n Ser IVet Asp Ala 90 Page 610 Ser lie Pro Val Ser Ala Leu 100 Gene Sequence 24-2-06.txt Asp Ser Leu Asn Asp Asn G n Ser Leu 105 110 Val Leu G u 145 Asp SIle G n Ser Ar g 225 Gy I e Lys Hi s Al a 305 Phe Al a Al a Met Ser 385 Thr Ser Gy Met Gy 115 Val Leu SIle Gy Thr 195 Leu Thr Al a Asp Lys 275 Leu Val Val vet Leu 355 Ar g Al a Pr o G u SIle 435 Val Leu Thr Asp G u 165 Al a Val Ser Ser Val 245 Ar g Leu Al a G n G u 325 Tyr Gy Gy Val Pr o 405 Al a Al a Al a Leu Asp 135 Ser Ser Ser Thr Gy 215 Ser Val Al a Gy Thr 295 Al a Pr o Ser SIle Leu 375 Leu SIle Al a l Ie Leu 455 Asp 120 Gy Pr o Ser Gy G u 200 Leu Leu Ser Hi s Val 280 Ser Asp Phe Thr bet 360 Val Al a Al a Thr Ser 440 Val Ser Asn Thr Thr G u 185 Leu Asn Leu Leu Ar g 265 Val Asp Tyr Gy Ser 345 Tr p Asn Phe et Ser 425 Leu Ser Thr Leu G u G u 170 Thr Val Val Lys Tr p 250 Phe Val Asp bet Al a 330 Ser Phe Gy Tr p Gy 410 Ar g Al a Asp Asp Val 155 G u G u Asp Asp Tyr 235 Thr Leu Al a Gy Al a 315 Pr o SIle Val Ar g Leu 395 Al a Leu Ser Al a Val 140 Asn Thr Gy Asp SIle 220 Al a Leu Pr o l Ie Phe 300 Asn Tyr Tr p SIle Ar g 380 Pr o Leu Leu Gy Thr 125 I le Ar g Thr Asp Pr o 205 G u Al a Hi s Al a Leu 285 I le Tyr Tyr Leu Thr 365 Val Tyr Leu Pr o Pr o 445 Asn Val Leu Ser G u 190 Ser I e l e Ar g Asn 270 Val vet Tyr Asp Ar g 350 Ar g Al a Asn Al a Al a 430 Thr Ar g Ar g Pr o Al a 175 Ar g Thr Asn Phe bet 255 Tr p Phe Thr Ar g Leu 335 Leu G u Hi s Asn Tr p 415 Al a Gy Leu Ser Ala Leu lie Arg Ile 460 Page 611 Gene Sequence 24-2-06.txt Leu 465 Ser Ser Thr Thr Val 545 Cys Gy Thr Gy Gy 625 I le Gy I le Val Lys 705 G u Al a Al a Gy Ser 785 Val Ar g Val Thr vet 515 Tyr Gy Al a Al a Phe 595 Tyr Ser Al a Tr p SIle 675 Val Al a G u Pr o vet 755 Gy Al a Ser Leu Pro Leu lie Gy Ala Ser Arg G y Ala Ser Gy 485 I le Ser G u Phe Val 565 Gy Met Gy Val Leu 645 Thr Gy SIle Al a Lys 725 Al a Lys Leu Leu Leu 805 470 Al a Leu SIle Ty r Thr 550 Val Pr o Met Leu Al a 630 Phe Ser I e I e G u 710 Ar g Gy Gy Ar g G u 790 Gy Ser SIle Ser Val 535 Ar g SIle Ser Phe Al a 615 Val Leu Ser G u Gy 695 Thr G u Val Phe Ser 775 Thr G u bet Al a Val 520 Ar g Ar g Al a Leu Thr 600 Gy Lys Leu Tyr Al a 680 Val Asn Ar g Ser Val 760 Leu Asn Ser Al a Val 505 Ar g Tyr Phe l Ie Ar g 585 Pr o Al a Ser Al a Al a 665 Ser SIle Hi s Al a Al a 745 Asp Thr Ser Leu 475 Leu Gy Al a Thr Val 555 Ar g et Lys Al a Ar g 635 Al a Pr o Val Ser vet 715 Ar g Val Tyr Asn Asp 795 Ser Al a Asp Lys Val 540 Leu Tyr vet Tr p Gy 620 bet Leu Tr p Val Phe 700 Gy Phe Val Pr o Thr 780 Ser G u Pr o G n Gy 525 bet et Gy Val Thr 605 Leu Ar g Al a Tr p Leu 685 Val G u Thr Leu Al a 765 Cys Phe Asp Leu 495 Leu Al a G n et SIle 575 Phe Hi s Al a SIle Val 655 Lys SIle Asp Val Leu 735 Thr Ser Leu Thr Ar g 815 Lys 480 Al a Ser Leu Thr Al a 560 Pr o Gy Phe Val Ser 640 Asn Thr Al a Val Al a 720 Al a Cys Val Al a Pr o 800 Gy Phe Ser Ala Ala Gy Ile Pro Pro Ser Ile Ser n Asp n Ala Asp Page 612 Gene Sequence 24-2-06.txt Leu Gy Val 865 Asn Thr Al a G u Leu 945 G u Ar g Val Pr o G u 1025 G n Ser Al a 835 G y Ser 850 Arg G y Leu Asp Sn Asn Thr G u 915 Hi s Tyr 930 G u Tyr Al a Mbet Tyr Pro Al a Thr 995 Pro Ar g 1010 Thr Pro Arg Thr Val Asp 3er Tyr Pr o 900 Su Asp Sy Vet Leu 980 Asp Val G y Phe 825 Sy Ala lle Ala Asn Thr 840 Su Ser Ser G y G n Ser 855 G u G y lie Asn G y Ser 870 Thr G n Val Pro Val Val 885 890 Ala Asn Ile Thr Thr Asp 905 Ala Pro Ile Ile Val Val 920 I e Asn y Val Arg n 935 Arg Leu Arg Asp Asn y 950 Tyr Asp lie Gy Pro u 965 970 Asp n Leu Pro u u 985 Val Asn Leu Asp G u As 1000 Pro Asn Leu Asp Ser L 1015 SLeu Leu Asp Trp Ala V 1030 Asp His Tyr Ala G y Va 1045 10 Asp His G y Gy Lys S 1065 G y y Ser [et G y T 1080 Pro Ser Tyr Leu Arg A 1095 Asp Asp Ser 845 Thr G y Asn 860 Asn Al a Ar g 875 G y Ser Trp Trp Tyr G u Ser Al a Al a 925 Ser G y G n 940 Asp Val G u 955 Pro Ser Tr p Thr G u Thr Thr G y G y Leu Pro Phe 880 Ser Al a G y 895 Ile Pro u 910 Sy Arg lle Ser Val bet Asp Leu G y 960 Arg Asn Leu 975 Ala Asp Val Val Arg Ile p G n Trp Val Ala Leu Thr eu al 50 er hr sn Asn Gy 1035 Thr Thr Al a Asp 1005 Asn Val 1020 Leu G n G u lie Leu Ser G u Al a 1085 Trp G y 1100 I e Phe Pr o Pr o 1070 Val Ar g .G y Pro G u 1055 Phe Asn Asp Arg Ile Asp Trp Al a Tyr 1090 Ser Pr o Ser Cys 1040 Tyr G n Asn Trp Al a 1120 Leu Al a 1075 G u 106C E y I I e G y Ser 1105 Pro Lys Trp Asn lie u Arg Val Ala Asp 1125 Pro G y Hi s 1140 Tyr Ser Leu Arg Thr 1110 Ile Asn Leu G u Thr 1130 IVbt Lys Val Asp G u 1145 Asn Ser Asn y Asp 1115 I e n Arg Ser y 1135 <210> 477 <211> 1509 <212> DNA <213> Corynebacter i um gl utam cum Page 613 Gene Sequence 24-2-06.txt <220> <221> CDS <222> (101)..(1486) <223> RXN01175 <400> 477 tcgcgtctct tagtcgatct gctcccat t c cataggt t gg ccattcctta aaacaccgat atcgtgctat gaataggggt gagaaaactt aactgttatt gat Asp at g bet cct Pr o tcg Ser aag Lys gac Asp aac Asn t cc Ser cct Pr o gat Asp 150 cct Pr o ttg Leu agc Ser t ac Tyr cct Pr o cag G n tgg Tr p cct Pr o gct Al a gcg Al a aag Lys cct Pr o 120 tct Ser gt c Val cgc Ar g cgc Ar g tgg Tr p 200 gct Al a gaa G u ttc Phe cgt Ar g at c SIle gtt Val act Thr ct g Leu at c SIle gat Asp ggt Gy at c SIle 170 ct c Leu cgc Ar g gct Al a ct c Leu gac Asp cag G n gtt Val aac Asn gag G u cag G n at g bet gat Asp 140 gag G u gct Al a agc Ser ttc Phe gag G0u 220 cct Pr o acc Thr gca Al a 45 gcc Al a ggt Gy t ca Ser at g Met gct Al a 125 gga Gy gca Al a t ac Tyr tct Ser gtt Val 205 at c SIle gat Asp at t SIle gaa G u cct Pr o aag Lys aac Asn gca Al a 110 cgt Ar g aat Asn acc Thr gct Al a ggc Gy 190 gcg Al a gac Asp cca Pr o cgt Ar g gt g Val gt a Val ttc Phe 80 gag G u gtt Val gct Al a ct g Leu gag G u 160 gct Al a gct Al a t cc Ser ggt Gy gt g Val 1 cca Pr o gac Asp cgc Ar g ctt Leu ct c Leu ccg Pr o ttg Leu ggt Gy cca Pr o 145 gct Al a tct Ser gat Asp cca Pr o ct g Leu 225 ttg Leu gct Al a aag Lys gag Gu ttg Leu cac Hi s acc Thr cag G n 130 aac Asn cgt Ar g gct Al a ctt Leu gct Al a 210 cgc Ar g agt tgg aca Ser Trp Thr cca Pr o aag Lys at c SIle ct g Leu cag G n at t SIle t ac Tyr 115 t ac Tyr t ac Tyr cgc Ar g gcg Al a t ac Tyr 195 ggt G y ttc Phe gaa G u cag G n ctt Leu aag Lys ggt Sy cgc Ar g 100 ggt Sy gca Al a cgt Ar g cac Hi s at g Met 180 cgt Ar g gca Al a at g bet gtt Val ggc G y caa G n gag G u cag G n ggt Sy gcc Al a gca Al a aag Lys ggc Sy gat Asp 165 aac Asn ct c Leu cgc Ar g gaa G u Page 614 Gene Sequence 24-2-06.txt gca tgt ggc gtg tcc Ala Cys Gy Val Ser 230 t cc Ser gca Al a cag G n aac Asn ggt Sy 310 aac Asn aag Lys gga Sy acc Thr gag G u 390 ggc Sy ggt Sy t cc Ser gag G u gat Asp tgg Tr p 280 gca Al a acc Thr gag Gu cgc Ar g aag Lys 360 t cc Ser cag G n at c SIle gct Al a tgc Cys 440 ttg Leu 250 gaa G u ggc Gy at g bet gaa G u ggc Gy 330 gt a Val at t SIle ggc Gy ttc Phe at t SIle 410 gac Asp cct Pr o gat gag tcc Asp G u Ser 235 ct g gtg gat Leu Val Asp ggc aac gag Sy Asn u gag cgc acc G u Arg Thr 285 atc tct aac I e Ser Asn 300 gag gct gtt G u Al a Val 315 cgt ttg acc Arg Leu Thr ctt cct ggt Leu Pro G y tgg cag tcc Trp G n Ser 365 tac aag acc Tyr Lys Thr 380 ttc gag gtc Phe G u Val 395 gag ttc act G u Phe Thr at c acc gat Ile Thr Asp cgc ctg aac Arg Leu Asn 445 t ac Tyr gaa G u 270 cgt Ar g cca Pr o gca Al a at c SIle gtt Val 350 gat Asp cgt Ar g cac Hi s ggt Gy gtt Val 430 act Thr ctg cgt gct gca gat Leu Arg Ala Ala Asp atc tac tgc I e Tyr Cys 245 240 cgt Ar g t ac Tyr at g bet ggc Gy gct Al a 320 gct Al a cag G n at g tet ttc Phe gca Al a 400 gat Asp ct g Leu cag G n t cc Ser gat Asp gat Asp at c SIle 305 gac Asp cgc Ar g gct Al a cac Hi s gac Asp 385 ttg Leu gt c Val cca Pr o tct Ser at g NMt ctt Leu gat Asp 290 aag Lys aag Lys at g bet gtt Val ggc Sy 370 aag Lys ggc Sy acc Thr ggc G y ttg Leu 450 ct g Leu t ca Ser 275 ttc Phe at t SIle ct c Leu ggc Gy gag G u 355 aac Asn gtt Val acc Thr gag G u cgc Ar g 435 gag G u cgt Ar g 260 gct Al a cat Hi s ggt G y gat Asp cac Hi s 340 gca Al a act Thr at c SIe cac Hi s tgc Cys 420 t ac Tyr ttg Leu 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1506 1509 ttc ctc gtt gca gaa atg ctg cgt aac Phe Leu Val Ala G u IVet Leu Arg Asn 455 460 aaa <210> 478 <211> 462 <212> PRT <213> Cor ynebact eri um gl ut am cum t aagct t t t a gaccaact ct Page 615 ene Sequence 24- 2- 06. txt <400> 478 Val Ser Trp Thr Val Asp Ilie Pro Lys Ou Val Leu Pro Asp Leu Pro Pr o As p Ar g Le u Le u Pr o Le u G y Pr o 145 A a Ser As p Pr a Le u 225 A a Ser As p As p I Ie 305 As p Ar g Pr a Ly s li1e Le u O31n I Ie Tyr 115 Tyr Tyr Ar g A a Tyr 1 95 O31y Ph e I Ie Le u Ser 275 Ph e I Ie Le u O31y O31y O31n O31u O31n O31y A a A a Ly s G31y As p 1 65 As n Le u Ar g (31u cys 245 Le u Hi s ValI Pr a Pr a 325 As p vb t Pr a Ser Ly s 70 As p As n Ser Pr a As p 150 Pr a Le u Ser Tyr A a 230 Ser A a O31n As n O31y 310 As n Ly s O31n Thr ValI Le u cys ValI Thr Ar g 135 li1e A a ValI O31u O31u 215 cys Hi s Thr Le u Ph e 295 li1e Ph e ValI O31n Tr p 40 Pr a A a A a Ly s Pr a 120 Ser ValI Ar g Ar g Tr p 200 A a O31y O31u As p Tr p 280 A a Thr O31u Ar g O31n As p Pr a As p O31u Thr 1 05 ValI Ser As n Nibt A a 1 85 As n Le u ValI A a O31u 265 li1e Ser Pr a Pr a Ser 345 O31u A a ValI A a 75 Ph e Le u Ly s Le u ValI 1 55 Ar g Thr O31u Ar g As p 235 Le u O31y O31u I Ie O31u 315 Ar g Le u As p O31n ValI As n O31u O31n vb t As p 140 O31u A a Ser Ph e O31u 220 O31u ValI As n Ar g Ser 300 A a Le u Pr a Thr A a A a O31y Ser vb t A a 125 G y A a Tyr Ser ValI 205 li1e Ser As p O31u Thr 285 As n ValI Thr O31y lIe O31u Pr a Ly s As n A a 110 Ar g As n Thr A a O31y 1 A a As p Le u Tyr O31u 270 Ar g Pr a A a I Ie ValI 350 Ser As n O31u A a Thr ValI I Ie G y Pr a As n 1 Thr As n Ser Ar g O31u 255 Le u Oy le Tyr ValI 335 li1e Ar g ValI ValI Ph e O31u ValI A a Le u O31u 1 A a A a Ser O31y A a 240 Ar g Tyr O31y A a 320 A a O31n Ala Val Ou Ala Ser Oy His Lys Val IlieTrp On Ser Asp Pro I'bt Page 616 ene Sequence 24 355 360 His (31y As n Thr Phe Thr Ala Se r As n G31y Tyr 370 375 Asp Lys Val Ilie Asp G31u Val (31n G31y Phe Phe 385 390 395 Leu G31y Thr Hi s Pr o 31y 31y Ilie His Ilie G31u 405 410 Val Thr G31u Cys Leu G31y G31y Ala G31u Asp Ilie 420 425 Pro G31y Ar g Tyr G31u Ser Ala Cys Asp Pr o Ar g 435 440 Ser Leu G31u Leu Ala Phe Leu Val Ala 31u I'b t 450 455 <210> 479 <211> 984 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> ODS <222> (961) <223> RXN01376 <400> 479 t cct cat cgg t tat cgggag t ggcggat ac t tct tgcaaa ct tcgt gagt act t tgaaat cccccat cgc t gt gat caca 2- 06. t xt 365 Lys Thr Ar g 380 (31u Val Hi s Phe Thr 03 y Thr As p Val 430 Leu Asn Thr 445 Leu Arg Asn 460 Hi s Phe Ar g Ala 400 31u As p 415 Asp Leu (31n (1n ct taaaccac t at gct t tcg gt g acc Val Thr t at t ca cca ggt G31y cga Ar g cct Pr o G31y Le u gt c ValI ct t Le u at c I Ie t ac Tyr ac c Thr c ag (31n aac As n gcg A a aac As n t gt Cys gag (31u gcg A a gt t ValI gca A a ggc G31y cgg Ar g g at As p aaa Lys5 g ac As p t cg Ser gt g ValI gcc A a t ac Tyr gag G31u 75 gt t ValI cgt Ar g ggt G31y tt c Ph e at g vb t t ca Ser gga G31y gca A a gt t ValI c ac Hi s t cg Ser ct g Le u gca A a cgc Ar g 45 ac g Thr gga G31y tt t Ph e cct Pr o gcg A a g at As p g ac As p ag c Ser gct A a gag G31u g ac As p g aa G31u 110 t at Tyr t ct Ser 15 aat As n aac As n at t I Ie at c I Ie g aa G31u 95 gca A a tt g Le u ggt G31y gt g ValI aat As n g at As p 80 g ac As p gga G31y 1 cct Pr o t ct Ser gag G31u at t lIe ggg G31y t ct Ser gcg A a ggt G31y gt g ValI tt c Ph e gcc A a gag G31u at t I Ie gt t ValI Tyr Ser Pr o gcg act t ca A a Thr Ser gac ggt gtt Asp 03 y Val ct c t ca act Leu Ser Thr gcc cga t cg Ala Ar g Ser t tc t tc ct c Phe Phe Leu gat caa tt g Asp 03 n Leu 1 00 ggc ccg ttg G31y Pr o Leu 115 ccg t cg gct cgg gcg gt a ccc Pro Ser Ala Arg Ala Val Pro Page 617 Gene Sequence 24-2-06.txt act ttg gcg aat ggc att ggt cac gct ttg Thr Leu Ala Asn G y lie G y His Ala Leu 135 140 tcc aat ccg tgg tcg gcg gct tac cgt gac Ser Asn Pro Trp Ser Ala Ala Tyr Arg Asp 150 155 gag cgc act gct ggc tgg ctg tcg gga tcg G u Arg Thr Ala y Trp Leu Ser y Ser 170 175 gat gcg ttt gat cga gtt ggt ggt ttt gat Asp Ala Phe Asp Arg Val Gy y Phe Asp 185 190 atg gaa gac gtt gac ctg gga gat cgg ctg IVet G u Asp Val Asp Leu y Asp Arg Leu 200 205 aac gtc ttt tgc cca agt gcg cag atc atc Asn Val Phe Cys Pro Ser Ala n lie lie 215 220 gcg ggt aaa aac cca gag aac atg ttg ccc Ala y Lys Asn Pro G u Asn IVet Leu Pro 230 235 tat cgc ttc cag gct gat cgc ctc gcg aag Tyr Arg Phe G n Ala Asp Arg Leu Ala Lys 250 255 cgg ttg gct ctg cga att ggt ttg aaa tta Arg Leu Ala Leu Arg Ile y Leu Lys Leu 265 270 ggt gtc tct aag atg aga acg aaa gcc tct Sy Val Ser Lys IVet Arg Thr Lys Ala Ser 280 285 gac <210> 480 <211> 287 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 480 Val Thr Tyr Ser Pro G y Lys Tyr Leu Al a 1 5 10 Pro G y Ala Thr Ser Arg Asp Thr His Val 25 Ser Val Asp G y Val Pro G u G n Ala Ala 40 G u Phe Leu Ser Thr G y G y Asn Leu G y 55 lie Ala Ala Arg Ser Leu Arg Ala Arg Arg 70 G y G u Phe Phe Leu Val Ser Asn Pro Asp 90 Pag ttg Leu gat Asp 160 tgc Cys gag G u gtt Val cac Hi s gca Al a 240 ccg Pr o cga Ar g ggt Gy 145 gaa G u ct g Leu cgc Ar g cgc Ar g gcg Al a 225 cac Hi s tgg Tr p gcc Al a 130 gct Al a gat Asp tta Leu t ac Tyr gcc Al a 210 aaa Lys cac Hi s caa G n gga Gy gt g Val at g tet tta Leu ttc Phe 195 ggt Gy ggt Gy gag G u gcc Al a gt c Val 275 tgg Tr p gac Asp agg Ar g 180 at g tet ttc Phe cat Hi s agc Ser cca Pr o 260 gcg Al a aaa Lys act Thr 165 tgg Tr p t ac Tyr acc Thr gtt Val gcg Al a 245 att SIe gtt Val tagaccgtcg acgaccaccg Ser Val Al a Tyr G u Val e 618 Phe Vet Ser Sy Al a Val Leu Al a Ar g Thr Gy Phe Asp Asp Ser Al a G u Asp Ser Asn Asn SIe SIe Su Gene Sequence 24-2-06.txt Ser IIe Asp n Leu Leu Gu Cys Ala Lys Arg His Pro Gu Ala y Al a Al a Gy 145 G u Leu Ar g Ar g Al a 225 Hi s Tr p Al a Gy 115 Al a Val bet Leu Phe 195 Gy Gy G u Al a Val 275 Leu Pr o Lys Thr 165 Tr p Tyr Thr Val Al a 245 l le Val SIle Thr Ser 150 G u Asp bet Asn Al a 230 Ty r Ar g Gy Ar g Leu 135 Asn Ar g Al a G u Val 215 Gy Ar g Leu Val G u 120 Al a Pr o Thr Phe Asp 200 Phe Lys Phe Al a Ser 280 105 Al a Asn Tr p Al a Asp 185 Val Cys Asn G n Leu 265 Lys Asp Gy Ser Gy 170 Ar g Asp Pr o Pr o Al a 250 Ar g bet Gy I e Al a 155 Tr p Val Leu Ser G u 235 Asp SIle Ar g Al a 125 Hi s Tyr Ser Gy Asp 205 G n Met Leu Leu Lys 285 Tyr Al a Ar g Gy Phe 190 Ar g SIle Leu Al a Lys 270 Al a 110 Pr o Leu Asp Ser 175 Asp Leu SIle Pr o Lys 255 Leu Ser Ser Leu Asp 160 Cys G u Val Hi s Al a 240 Pr o Ar g <210> 481 <211> 1002 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(979) <223> RXN01631 <400> 481 gccagttgag gatgctctca cagtcgctcg cattgtcagt caaccagggc atttccgtaa acatctaaga ggagcactcc at t SIle gat Asp gaa gau gca tct Ser agc Ser ggt Gy gac tgg Tr p cca Pr o gca Al a at t acc Thr gt a Val ggc gcy ggc agc Ser gca Al a at t SIle t ac gcg Al a at c l Ie ggg Gy 45 gag gga Gy gat Asp ctt Leu gaa gac gcc Asp Al a 15 gag cgc G u Arg gtt cac Val Hi s ttg cgc Page 619 gcatgctacg aatccgacaa atg aaa cca caa ctt IVet Lys Pro G n Leu 1 gca ccc gat cgt gac Ala Pro Asp Arg Asp atc gct cta gtc gct Ile Ala Leu Val Ala gcc gat ctc atc aaa Ala Asp Leu Ile Lys cga cgc atc cac gct Al a Ar g Asp Thr Ile G y gca Al a act Thr gct Al a gag G u act Thr gaa G u 150 gtt Val tgg Tr p at t SIle gac Asp tgc Cys 230 gac Asp cac Hi s acc Thr at t l Ie gcg Al a gcg Al a acc Thr 120 ct c Leu act Thr agc Ser acc Thr ct g Leu 200 cca Pr o gaa G u ggt Gy agc Ser gca Al a atc at t le le 75 cgc caa Arg G n gct ct t Al a Leu ggt gtg G y Val gcg gaa Al a G u ttt tct Phe Ser 155 t cc gat Ser Asp 170 aaa at c Lys II e aag gt c Lys Val gat ct t Asp Leu gaa ttt G u Phe 235 acg ggc Thr G y 250 ccg gt g Pro Val ttt gaa Phe G u Tyr 60 gag G u gag G u ggt Gy gtt Val gct Al a 140 cac Hi s agc Ser gga G y aac Asn ttc Phe 220 gat Asp gca Al a aaa Lys caa G n Gene Sequence 24-2-06.txt G u G u Leu Arg Arg Arg I I e Hi s Al a gt g Val t cc Ser t cc Ser ccc Pr o 125 gaa G u ct g Leu cca Pr o at c SIle gca Al a 205 gat Asp ccc Pr o t at Tyr gaa G u gct Al a 285 gag G u gat Asp cca Pr o 110 at t SIle gct Al a aag Lys tcg Ser ccc Pr o 190 gt g Val gac Asp gca Al a ggt Gy ggg G0y 270 ttc Phe gcc Al a 95 cac Hi s gct Al a cat Hi s acc Thr gct Al a 175 aac Asn gag G u t cc Ser agc Ser gt g Val 255 ct g Leu ct c Leu gtt Val at t SIle cac Hi s ggc Gy at c SIle 160 gga G y gat Asp gtt Val acc Thr ttc Phe 240 gaa G u caa G n ggt G y gcg Al a gt c Val gcc Al a 130 aag Lys gac Asp at g let ct g Leu gat Asp 210 cgt Ar g cgt Ar g at t SIle gct Al a tgg Tr p gat Asp gga Gy 115 agt Ser ct c Leu gcg Al a gtt Val tgg Tr p 195 ggt G y cgc Ar g ggc G y t cc Ser ttc Phe 275 tgg Tr p ctg Leu 100 gca Al a gcg Al a gcg Al a ctg Leu gat Asp 180 cgc Ar g ttc Phe gcg Al a gcc Al a gca Al a 260 gac Asp gct cgt ctc gcc ccc tcc act aac Ala Arg Leu Ala Pro Ser Thr Asn 280 tgatctttga aaggctgaaa aaa <210> 482 <211> 293 <212> PRT <213> Corynebacter i um gl utam cum <400> 482 1002 Page 620 IVet Lys Pro n Leu Ile Ala 1 5 Gene Sequence 24-2-06. t xt Ser Cys Trp Thr Ser Al a G y Asp Al a 10 Al a SIle Al a Ar g Asn Ar g Lys bet Val 145 Tyr Leu G u Asp Asn 225 I e I e Ar g Al a Asp Arg Asp Leu Val Al a Leu Ile Lys Ile His Al a Trp Trp Al a Asp Leu Phe 100 G y Al a G y 115 Ser Al a Phe Leu Ala Leu Al a Leu G u 165 Val Asp Ile 180 Trp Arg Asn 195 G y Phe Ile Arg Al a Tyr G y Al a lile 245 Ser Al a G u 260 Phe Asp Thr 275 Ser Thr Asn Asp G u Al a Al a 70 Thr Al a G u Thr G u 150 Val Tr p SIle Asp Cys 230 Asp Hi s Thr Leu Thr Ar g Gy Gy Al a Gy Asp 135 Al a Val Hi s Pr o Thr 215 Gy Al a Ar g Ser Gy 40 Asp SIle Al a Al a Thr 120 Leu Thr Ser Thr Leu 200 Pr o G u Gy Ser Ser 25 Tr p Thr G u G u G n 105 Asn Al a Pr o Hi s Al a 185 Ser SIle Gy Tr p Leu 265 Pr o Al a SIle SIle Ar g 90 Al a Gy Al a Phe Ser 170 Lys Lys Asp G u Thr 250 Pr o Val Gy Gy SIle G n Leu Val G u Ser 155 Asp SIle Val Leu Phe 235 Gy Val Al a SIle Tyr G u G u Gy Val Al a 140 Hi s Ser Gy Asn Phe 220 Asp Al a Lys l Ie Gy G u Val Ser Ser Pr o 125 G u Leu Pr o SIle Al a 205 Asp Pr o Tyr G u Asp Leu G u G u Asp Pr o 110 SIle Al a Lys Ser Pr o 190 Val Asp Al a Gy Gy 270 G u Val Leu Phe Al a Hi s Al a Hi s Thr Al a 175 Asn G u Ser Ser Val 255 Leu lie Ala Ala Phe Ou n Ala Ala Arg Leu <210> <211> <212> <213> <220> <221> <222> <223> 483 990 DNA Cor ynebact eri um gl ut ari cum CDS (967) RXN01593 Page 621 Gene Sequence 24-2-06.txt <400> 483 atatgacagc cttcttcttg atctagatgg aaccgtctac gagggcggcc gagccattga gcacgtagtt tctgcgctct ctggcgccgg cctacccgtc atg tat gtc acc aat IVet Tyr Val Thr Asn aat Asn ggc Gy gcc Al a gtt Val gt g Val cac Hi s at t SIle ccc Pr o gt g Val ccc Pr o 150 ttg Leu gca Al a gat Asp ct c Leu cca Pr o 230 t cc Ser gcc Al a aag Lys ggt Gy gtt Val cct Pr o gct Al a gaa G u 120 aac Asn at g et gt c Val at g let gt g Val 200 gca Al a cgg Ar g gct Al a acc Thr gcg Al a gaa G u tcg Ser acc Thr gcg Al a ggt Sy act Thr t at Tyr gat Asp 170 aca Thr gcc Al a tct Ser ttc Phe ccg Pr o act Thr gcg Al a t cc Ser gct Al a 75 ggt G y cag G n cgt Ar g ggc G y gcg Al a 155 cgt Ar g tt c Phe at t l Ie ct a Leu agc Ser 235 gag G u gcg Al a gag G u ttc Phe gat Asp tgg Tr p t at Tyr cac Hi s gt a Val 140 ggg G y ct c Leu cag G n ccc Pr o cag G n 220 gct Al a gt g Val gac Asp aag Lys 45 cgc Ar g gat Asp gct Al a ttt Phe at t SIle 125 aag Lys gct Al a gat Asp gt c Val aga Ar g 205 cga Ar g t at Tyr gt g Val aat Asn at t SIle gag G u aaa Lys cag G n gca Al a 110 ggc G y cct Pr o gaa G u acc Thr ct g Leu 190 gca Al a t cc Ser cga Ar g gct Al a 15 gt g Val ccc Pr o ct a Leu cct Pr o ttg Leu 95 t ca Ser aac Asn ctt Leu act Thr gat Asp 175 acc Thr gcg Al a ggg G y cgg Ar g gcg Al a at g Net gct Al a gct Al a gt g Val 80 agc Ser aat Asn ggt G y t cc Ser ctt Leu 160 at c SIle ggc G y ccc Pr o cga Ar g cga Ar g 240 1 caa G n aca Thr gga G y act Thr gct Al a gag G u ttg Leu t cc Ser gca Al a 145 aat Asn gcc Al a gt c Val cac Hi s gct Al a 225 cac Hi s ct c Leu tct Ser t cc Ser gaa G u gt g Val gct Al a gat Asp at g let 130 ggt G y tct Ser ggc G y agc Ser ct a Leu 210 caa G n cct Pr o cgt Ar g gct Al a aag Lys gct Al a ctt Leu gcg Al a t cc Ser 115 gt g Val aag Lys t ca Ser gga G y ggc G y 195 t at Tyr gcc Al a ggt G y gag G u caa G n gt g Val ggt G y cac Hi s ct g Leu 100 acc Thr gct Al a cca Pr o aag Lys aac Asn 180 t ac Tyr cgc Ar g agg Ar g cat Hi s att SIe gct Al a t at Tyr ttt Phe ggc G y t ca Ser ctt Leu gcc Al a ggc G y cct Pr o 165 gct Al a t ac Tyr cac Hi s tgc Cys ttc Phe 245 cgg cgg cga tgc cgg cgc aac tcc ggt tgc agc act ccg cac tgc gtt Page 622 Arg Arg Arg Cys Arg Arg Asn Ser C 250 gga tgt ggc ctg ggc ggc cac aga g Sy Cys G y Leu G y Gy His Arg A 265 2 tca gag gta gct gct act gca ttg c Ser G u Val Ala Ala Thr Ala Leu C 280 285 attcaccaaa gcc <210> 484 <211> 289 <212> PRT <213> Cor ynebact eri um gl ut am cum Sequence 24-2-06. t xt 3y Cys Ser Thr Pro His Cys Val 255 260 ica gtc acc gag gta cgc gct gat I a Val Thr G u Val Arg Ala Asp 70 275 ag agc tgg tgg taaacggtga Sn Ser Trp Trp <400> 484 lMt Tyr Val 1 G n Leu Ar g Thr Ser Al a G y Ser Lys Thr G u Al a Al a Val Leu G u Ala Al a Leu Asp Ser 115 Ser lMt Val 130 Al a G y Lys 145 Asn Ser Ser Al a G y G y Val Ser G y 195 Hi s Leu Tyr 210 Ala G n Al a 225 His Pro G y Thr Asn 5 G u Ile G n Al a Val Tyr G y Phe Hi s G y Leu Ser 100 Thr Leu Al a Al a Pro G y Lys Pr o 165 Asn Al a 180 Tyr Tyr Arg Hi s Arg Cys Hi s Phe 245 Asn Ala Ser Arg Leu Cys Leu 55 Val Asn Asn lbet Val 135 Al a Al a Gy Leu Asp 215 Gy Ar g Al a Lys 40 Gy Val Pr o Al a G u 120 Asn let Val let Val 200 Al a Ar g Ar g Asp Met Ser Asp G u Gy 105 Ar g Al a Phe Gy Asp 185 Ar g Gy Phe Cys Al a 10 Thr Al a G u Ser Thr 90 Al a Gy Thr Tyr Asp 170 Thr Al a Ser Phe Ar g 250 Pr o Thr Al a Ser Al a 75 Gy G n Ar g Gy Al a 155 Ar g Phe l Ie Leu Ser 235 G u Al a G u Phe Asp Tr p Tyr Hi s Val 140 Gy Leu G n Pr o G n 220 Al a Val Asp Lys Ar g Asp Al a Phe SIle 125 Lys Al a Asp Val Ar g 205 Ar g Tyr Al a Al a Val let Pro Al a Leu Al a Pro Val Leu Ser Ser Asn Asn y Leu Ser Thr Leu 160 Asp I e 175 Thr G y Ala Pro G y Arg Arg Arg 240 Cys Ser 255 Arg Asn Ser y Page 623 Gene Sequence 24- 2- 06. t xt Thr Pro His Cys Val y Cys Gy Leu G y Gy His Arg Ala Val Thr 260 265 270 G u Val Arg Ala Asp Ser G u Val Ala Ala Thr Ala Leu G n Ser Trp 275 280 285 Tr p <210> 485 <211> 1173 <212> DNA <213> Cor ynebact er i um gl ut am cum <220> <221> CDS <222> (101)..(1150) <223> RXN00337 <400> 485 ggacgcttat tggtgagcat tcggattacg ccggtggtgt gg attgccggac tgcggctgcc gtcaataaag aaccgcgacg at tgctggcg gctaatgcga ttg tta acg tat Leu Leu Thr Tyr gcg Al a gcg Al a ttg Leu cgg Ar g cgt Ar g ggg Gy gcg Al a gcg Al a ttg Leu tcg Ser 150 gt g Val at t l Ie cgg Ar g ggc G y aca Thr ggg Gy t at Tyr at t l Ie 120 gcg Al a gtt Val gt g Val gct Al a act Thr at t SIle ggc G y ggt Gy gaa G u tcg Ser cac Hi s t ac Tyr gag G u cgc Ar g acg Thr gcg Al a ct g Leu 75 gaa G u aat Asn cag G n acc Thr gcc Al a 155 gga G y gca Al a ccg Pr o tgg Tr p gat Asp aat Asn at t SIle t cc Ser gt g Val 140 gat Asp ggc G y cac Hi s cct Pr o 45 aca Thr at c SIle t cc Ser gag G u gca Al a 125 gcg Al a ggt Gy gt c Val gcc Al a cag G n at g Met acc Thr gcc Al a gaa G u 110 ttc Phe ttg Leu tcg Ser gaa G u 15 cat Hi s ccg Pr o at c SIle gt g Val at g Met 95 gcc Al a at g Met cgg Ar g gt c Val aag Lys at g Met gag G u cat Hi s ttg Leu 80 gat Asp ccc Pr o ttc Phe ggt Gy act Thr 160 tct Ser t cc Ser ggc G y caa G n acc Thr gcg Al a aag Lys gag G u 130 act Thr gcg Al a tta Leu cat Hi s gtt Val at g Met at t SIle ct c Leu gcg Al a 115 act Thr gga G y cca Pr o agc Ser gat Asp gct Al a ctt Leu cct Pr o gca Al a 100 cgc Ar g t ca Ser cag G n cat Hi s act Thr gtt Val gcc Al a tcg Ser gag G u ttg Leu att SIe gtg Val att SIe ccg Pr o 165 gtg agt cgt tcc gct ggt ttg tcg gca ttt gtt gtt gct gcg caa act Page 624 Gene Sequence 24-2-06.txt Val Ser Arg Ser Ala G y Leu Ser Ala Phe Val Val Ala Ala G n Thr 170 175 180 gaa act gat ccg agc att tac cgc gag atc tat gct cga cat gcg ttt 691 G u Thr Asp Pro Ser Ile Tyr Arg G u Ile Tyr Ala Arg His Ala Phe 185 190 195 atc gat gaa gct gcg cgc gct ttc agt gtg gaa tct ttg cgg ttg ctt 739 Ile Asp G u Ala Ala Arg Ala Phe Ser Val u Ser Leu Arg Leu Leu 200 205 210 ccc gac gct tcc act cgt gtt gtg gat tgg ttg cag gcc gtg att gag 787 Pro Asp Ala Ser Thr Arg Val Val Asp Trp Leu G n Ala Val Ile G u 215 220 225 gtg act ggt cga gag gat ctg ccc tcg att gaa caa gcc cag cgc tgg 835 Val Thr y Arg u Asp Leu Pro Ser Ile G u On Ala On Arg Trp 230 235 240 245 ttg aat ctg tgg gaa aac gaa acc cgg cgc gct cag agg aca gcc aat 883 Leu Asn Leu Trp G u Asn G u Thr Arg Arg Ala G n Arg Thr Ala Asn 250 255 260 gcc ctg cgt tcg aga agg ctg agt gag ttt tct gag ctg ctg atg gaa 931 Ala Leu Arg Ser Arg Arg Leu Ser G u Phe Ser G u Leu Leu IVet G u 265 270 275 tcc caa gat gat ttg agc gac acc ttc gat ttc ccc cct gct gat ttg 979 Ser G n Asp Asp Leu Ser Asp Thr Phe Asp Phe Pro Pro Ala Asp Leu 280 285 290 gcg ctt gct cgt ttg tgc gtc gag cgg ggt gcc aca gct gct cgg tcc 1027 Ala Leu Ala Arg Leu Cys Val G u Arg y Ala Thr Ala Ala Arg Ser 295 300 305 acg tca gcg cgc ggt gtg att gcg ttg gtt gat gcc cat cat gcg cac 1075 Thr Ser Ala Arg y Val Ile Ala Leu Val Asp Ala His His Ala His 310 315 320 325 aat ttt gct gcg gat ctc agc gag gat ggc ttg ttg gtg gtt cct ctc 1123 Asn Phe Ala Ala Asp Leu Ser G u Asp y Leu Leu Val Val Pro Leu 330 335 340 ggg cac ggg gac gtc gcg gaa cag ggc tagcacgcct acttaaccag 1170 G y Hi s y Asp Val Al a u n G y 345 350 cct 1173 <210> 486 <211> 350 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 486 Iet Leu Leu Thr Tyr Al a Phe Val Asp Val G u G y y Val G u Lys 1 5 10 His Ser Leu Ser Thr Ala Asp Ile Ala Ala Arg Ala His Ala His IVet 25 Lys Ser His Asp Val Leu y Arg G n Thr Thr Pro Pro n Pro u 40 G y G y Val Ala Ala Arg Leu G y G y IIe Ala Trp Thr Iet IIe His 55 Page 625 Lys G n IVet Leu Ser Arg Gene Sequence 24-2-06.txt Asp Thr Lys y Leu Asp Ile Thr Val Leu Ser Val Thr Ser G u 145 G n Val Al a Ser G n 225 G n G n G u Pr o Thr 305 Al a Leu SIle Leu Al a 115 Thr Gy Pr o Al a Hi s 195 Ar g Val G n Thr Leu 275 Al a Al a Hi s Val G u Leu l Ie Val SIle Pr o 165 Thr Phe Leu G u Tr p 245 Asn G u Leu Ser Hi s 325 Leu Sy Al a Al a Leu Ser 150 Val Su I e Pr o Val 230 Leu Al a Ser Al a Thr 310 Asn Sy Val Leu G u Ar g 135 Val Ser Thr Asp Asp 215 Thr Asn Leu G n Leu 295 Ser Phe Hi s Sy Tyr SIle 120 Al a Val Ar g Asp G u 200 Al a Sy Leu Ar g Asp 280 Al a Al a Al a Sy Leu Ar g 105 Cys Ar g Asp Ser Pr o 185 Al a Ser Ar g Tr p Ser 265 Asp Ar g Ar g Al a Asp 345 Sy 90 G u Ser Hi s Tyr Al a 170 Ser Al a Thr G u G u 250 Ar g Leu Leu Sy Asp 330 Val G u Asn G n Thr Al a 155 Gy I e Ar g Ar g Asp 235 Asn Ar g Ser Cys Val 315 Leu Al a Asn SIle Ser Val 140 Asp Leu Tyr Al a Val 220 Leu G u Leu Asp Val 300 I le Ser G u Ser G u Al a 125 Al a Sy Ser Ar g Phe 205 Val Pr o Thr Ser Thr 285 G u Al a Su Sn Al a G u 110 Phe Leu Ser Al a G u 190 Ser Asp Ser Ar g G u 270 Phe Ar g Leu Asp Sy 350 vet Al a Met Ar g Val Phe 175 I e Val Tr p SIe Ar g 255 Phe Asp Sy Val Sy 335 <210> 487 <211> 1248 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1225) <223> RXS00584 <400> 487 tagttgtgcc acctaaaacg cgaacagaac cggagtcgag cagcacctcc ccgcaagggt Page 626 Gene Sequence 24 agaggggctg cttttttgtt tcctaaattc accccatccc -2-06. txt agg Ar g acc Thr gat Asp gag G u cgc Ar g gcc Al a cag G n at c SIle t ac Tyr gtt Val 150 agc Ser cgt Ar g at g Net gt c Val t cc Ser 230 t cc Ser gaa G u aac Asn at c SIle gat Asp gtt Val gat Asp ct c Leu gga Gy 120 at c SIle aac Asn cag G n acc Thr at t SIle 200 gcg Al a gac Asp at c SIle at g NMt cgc Ar g gca Al a cag G n gtt Val gca Al a at t SIle aag Lys gag G u gat Asp t ac Tyr 170 tct Ser ttc Phe cag G n gcg Al a ttg Leu 250 agt Ser gt c Val aac Asn gac Asp gt g Val 75 aac Asn gt c Val gga Sy ggc Sy ct c Leu 155 gcc Al a cag G n aag Lys gct Al a ct g Leu 235 cgc Ar g tct Ser gt g Val cca Pr o at c SIle gga Gy cgc Ar g at g tet ttg Leu ttg Leu 140 cca Pr o gac Asp gt g Val aac Asn gcc Al a 220 agc Ser ggc Gy cca Pr o gct Al a ct g Leu 45 gct Al a cct Pr o ct g Leu cgc Ar g at c SIle 125 cgc Ar g gt c Val act Thr cac Hi s gga Gy 205 cag G n gt c Val ggt G y gt c Val ttc Phe aca Thr gat Asp tgc Cys gct Al a gt g Val 110 aat Asn at t SIle ggc Sy gt c Val cgc Ar g 190 act Thr aac Asn gt g Val acc Thr t ca Ser 15 cac Hi s cca Pr o at c SIle t ca Ser ccg Pr o 95 t ac Tyr gat Asp gcg Al a tgc Cys gca Al a 175 cag G n gac Asp cca Pr o gag G u t cc Ser 255 ct c Leu gag G u aag Lys ttc Phe gtt Val 80 ct g Leu ttc Phe cct Pr o cgc Ar g gaa G u 160 tgg Tr p ct g Leu gga G y cac Hi s acc Thr 240 ggc G y at g let 1 gaa G u ct g Leu cag G n gct Al a cac Hi s gca Al a gag G u cac Hi s aaa Lys 145 ttc Phe gga Sy gct Al a aac Asn ttc Phe 225 gca Al a ccg Pr o aac Asn cct Pr o gct Al a ggc G y gat Asp aag Lys aag Lys ct c Leu 130 gt g Val ct c Leu gca Al a tct Ser at c SIle 210 ttc Phe ggc G y aat Asn gcg Al a agc Ser t cc Ser gac Asp cct Pr o cgc Ar g cct Pr o 115 aac Asn ctt Leu gaa G u at c l Ie ggg G y 195 cag G n ttc Phe aac Asn cat Hi s gcg Al a cct Pr o aag Lys gat Asp gaa G u ctt Leu 100 cgc Ar g gaa G u at c SIle cca Pr o ggc Sy 180 at g Net gtt Val gga G y agc Ser gat Asp 260 cat agc cct gaa His Ser Pro Gu t ca Ser aca Thr gtg Val gac Asp gca Al a gat Asp acc Thr acc Thr gac Asp aac Asn 165 gct Al a tct Ser gca Al a acc Thr aac Asn 245 gca Al a Page 627 Gene Sequence 24- 2- 06. txt gct tcg gtg gag gcc gtc gtc gag aag ctt ggt gaa aac gct Ala Ser Val G u Ala Val Val G u Lys Leu Gy G u Asn Al a 265 270 275 atg atc gat gct tcc cat gct aac tcc ggc aag gat cat atc bt I Ie Asp Ala Ser His Ala Asn Ser G y Lys Asp His lie 280 285 290 gtt gag gtt gtt cgt gaa atc gca gag cag att tct ggc ggt Val u Val Val Arg u Ile Ala u n Ile Ser Gy y 295 300 305 gct gtg gct gga atc atg att gag tcc ttc ctc gtt ggt ggc Ala Val Ala Gy Ile IVet I e u Ser Phe Leu Val Gy y 310 315 320 aac ctt gat cct gcg aaa ttg cgc atc aat ggc ggt gaa ggc Asn Leu Asp Pro Al a Lys Leu Arg lie Asn G y G y G u G y 330 335 tac gga cag tct gtg acc gat aag tgc atc gat att gac acc Tyr y G n Ser Val Thr Asp Lys Cys Ile Asp Ile Asp Thr 345 350 355 gat ttg ctc gct gag ctg gcc gca gca gta agg gaa cgc cga Asp Leu Leu Ala G u Leu Ala Ala Ala Val Arg G u Arg Arg 360 365 370 gcc aag taattaaggg cgctagactg tta Ala Lys 375 <210> 488 <211> 375 <212> PRT <213> Corynebacter i um gl ut ai cum cgt Ar g cga Ar g tct Ser gca Al a ct g Leu 340 acc Thr gca Al a ct c Leu cag G n gaa G u cag G n 325 gt g Val at c SIe gca Al a 931 979 1027 1075 1123 1171 1219 1248 <400> 488 IVet His Ser Pro u Arg On u Lys 1 G u Leu G n Al a Hi s Al a G u Hi s Lys 145 Al a Ser Ser Asp Pr o Ar g Pr o 115 Asn Leu 5 Ser Thr Val Asp Al a Asp Thr Thr Asp Ser Leu G n Leu l Ie Asp Val Asp 135 Val Lys Al a Ar g Val Tyr Lys 105 Tr p Pr o Leu Met Ser 10 Arg Val Al a Asn I Sn Asp I Val Val Al a Asn 90 Ile Val I Lys G y I G u G y I Asp Leu I 155 Page 628 Pr o Al a Leu Al a Pr o Leu Ar g SIle 125 Ar g Val Ser Hi s Pr o SIe Ser Pr o Tyr Asp Al a Cys ene Sequence 24- 2- 06. txt Phe Leu O31u Pro Asn Ser Pro O31n Tyr O31y Al a As n Ph e 225 Al a Pr o O31u As p Ser 305 ValI G31y I Ie aOu I Ie G31y 1 95 O31n Ph e As n Hi s Al a 275 li1e G31y G31y G31y Th r 355 Ar g 1 65 Al a Ser Al a Th r As n 245 Al a Le u G n O u 0On 325 ValI I Ie Ar g vb t ValI Ser 230 Ser Al a vb t ValI Al a 310 As n Ty r As p Th r Pr o As p 215 As p Hi s Ser I Ie G u 295 ValI Le u 0Gy Le u O31u 1 G31y ValI Gy le Ou 265 Al a ValI G31y Pr o Ser 345 Ty r 1 70 Ser Ph e O31n Al a Le u 250 Al a Ser Ar g I Ie Al a 330 ValI O31n Ly s Al a Le u 235 Ar g ValI Hi s G u vb t 315 Ly s Th r Hi s G31y 205 O31n ValI O31y O31u As n 285 Al a O31u Ar g Ly s Al a 365 Ala Asp Thr Val Ala Trp 1 O31n As p Pr a O31u Ser 255 Le u O31y G n Ph e As n 335 li1e Al a 0u Leu Al a Al a ValI Ar g Al a Al a Al a <210O> 489 <21 1> 1131 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1108) <223> RXS02574 <400> 489 t gt gct cct t gcgggct gcg cagaagagcc ggaacagcaa aaagcaat aa gccgct t at c gacgtccccc tccacccctc ccgcaccgac cgcggaggat ttg gcg cgc gcg caa 115 Leu Al a Ar g Al a O31n 1 atc cct gaa cag caa cgc gac caa gtc gcg tcg ct gat gat ggt t gga 163 Ilie Pro3u On OnArgAspOnVai Al aSer Leu 'b t I'b t Val O31y 15 gt t gcg aat t at gat cag gca t tg gat gcg ct c aat cag ggg gt g ggt 211 Val Aa Asn Tyr Asp (1n Aa Leu Asp Aa Leu Asn (1n (1y Val (3y 30 Page 629 Gene Sequence 24-2-06.txt ggc Gy ggc G y gt c Val ctt Leu gaa G u cat Hi s ggt Gy gca Al a 150 at t SIle gat Asp act Thr gcc Al a gtt Val 230 gat Asp ct c Leu gt g Val t at Tyr ttt Phe aat Asn at c SIle gat Asp gt a Val gt g Val 120 ccc Pr o act Thr cca Pr o cac Hi s gac Asp 200 at g bet t cc Ser cca Pr o gga G y gcc Al a 280 tcg Ser ggt Gy gag G u ttc Phe ccc Pr o gat Asp gtt Val gtt Val gcc Al a ttc Phe 170 caa G n at c SIle ggc G y at c SIle ggc G y 250 agt Ser ct c Leu ggc G y t cc Ser gcg Al a gaa Gu 75 t ca Ser ct c Leu aac Asn ggc Gy aca Thr 155 aaa Lys gat Asp cct Pr o cac Hi s gac Asp 235 gt g Val gcc Al a aaa Lys t cc Ser tgg Tr p ct c Leu ggc G y ccg Pr o gca Al a ttt Phe gat Asp 140 gct Al a cat Hi s gt g Val t at Tyr at g bet 220 ccc Pr o cct Pr o at t l Ie gca Al a gcg Al a 300 aca gat gaa Thr Asp G u aat Asn 45 cgc Ar g ggc G y cgc Ar g gaa Gu gca Al a 125 cgt Ar g ttt Phe ttc Phe gt g Val ggt Gy 205 at t SIle gcc Al a ttc Phe t cc Ser ggc G y 285 at t l Ie gaa gcc gt c G u Al a Val cgc gtc cag Arg Val G n 80 gtg atg gcg Val Net Ala 95 at c ct a ggc Ile Leu G y 110 cct gtt gt a Pro Val Val tcc ttt tcc Ser Phe Ser gca aag ggc Al a Lys G y 160 cca ggt cac Pro G y Hi s 175 acc ccc gca Thr Pro Al a 190 caa gca ctt G n Al a Leu gtt cca ggt Val Pro y acc tat caa Thr Tyr G n 240 gat ggc gt g Asp G y Val 255 gcc acc cat Al a Thr Hi s 270 gca gac caa Al a Asp G n gat cgc gtt Asp Arg Val ct g ct c Leu Leu ggc agg G y Arg cgt gcc Arg Ala caa acc G n Thr act ggt Thr G y gat gta Asp Val 130 aac gac Asn Asp 145 tta agc Leu Ser ggt cgt G y Arg ctt gat Leu Asp tct gaa Ser G u 210 ctt ggc Leu G y 225 ct g ct c Leu Leu atc tac Ile Tyr t ca ccc Ser Pro gca ct a Al a Leu 290 acg gaa cct Thr G u Pro gat Asp acc Thr at g tet tta Leu 115 gat Asp cca Pr o aaa Lys gca Al a gag G u 195 act Thr acc Thr cgc Ar g acc Thr gca Al a 275 tgg Tr p ttc Phe aat Asn acg Thr 100 gct Al a gct Al a gcc Al a gt a Val agt Ser 180 ctt Leu gac Asp gac Asp agt Ser gac Asp 260 gaa G u at c SIle t cc Ser at t I e ccg Pr o gca Al a tgg Tr p gt a Val gga Gy 165 ggc Gy aaa Lys gga Gy gga G y ggc G y 245 gat Asp gca Al a gac Asp 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 gat Asp 305 gct gcc gtt agc Ala Ala Val Ser Page 630 ene Sequence 24- 2- 06. t xt agc ggt gaa t ac cct caa gaa caa atg ctg gca t ct gcg t ta aga gt c 1075 Ser O31y O31u Ty r Pro O31n O31u O31n I'bt Leu Al a Ser Al a Leu Ar g Val 310 315 320 325 caa t tg ct c t ac at c aca cgt ct c gaa caa aag t gaagt tacc agt ccgt aac 1128 O31n Leu Leu Tyr Ilie Thr Ar g Leu O31u O31n Lys 330 335 ccc 1131 <210> 490 <211> 336 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn Le u Le u As n Le u O31y Ar g O31n Thr As p As n 145 Le u O31y Le u Ser Le u 225 Le u I Ie O> 490 Ala Arg Ala O1n Ilie Pro Ou On vb t O31y Thr As p Thr Nibt Le u 115 As p Pr o Ly s Al a O31u 1 95 Thr Thr Ar g Thr O31y O31y Pr o Ser lIe Pr o Al a Tr p ValI O31y 1 65 O31y Ly s O31y O31y O31y 245 As p ValI O31y O31y ValI 70 Le u O31u Hi s O31y Al a 150 li1e As p Thr Al a ValI 230 As p Le u Al a I Ie Ar g Ser O31y O31n O31y Le u 135 Al a Thr Ser Tyr ValI 215 Pr a Tyr Ser Tyr As p Ph e O31u 1 Thr ValI Tyr ValI Thr 1 Le u ValI Ser O31y 265 Ar g O31n Ser Al a O31u Ser Le u As n O31y Thr 1 Ly s As p Pr a Hi s As p 235 ValI As p Al a Tr p Le u O31y Pr a Al a Ph e As p 140 Al a Hi s ValI Tyr vb t 220 Pr a Pr a O31n Le u Thr Ar g O31y Ar g O31u Al a 125 Ar g Ph e Ph e ValI O31y 205 li1e Al a Ph e Al a Al a O31u Al a ValI vb t Le u ValI Ph e Ly s O31y 1 Pr a Al a Pr a Ty r O31y 255 Ser Ala Ilie Ser Ala Thr His 270 Page 631 Gene Sequence 24-2-06.txt Ser Pro Ala u Ala Val Leu Ala Ser Leu Lys Ala y Ala Asp n 275 280 285 Ala Leu Trp lie Asp Tyr y Ser Leu y Ser Ala lie Asp Arg Val 290 295 300 Asp Ala Ala Val Ser Ser Gy u Tyr Pro n u n IVet Leu Ala 305 310 315 320 Ser Ala Leu Arg Val G n Leu Leu Tyr Ile Thr Arg Leu G u G n Lys 325 330 335 <210> 491 <211> 1038 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (1)..(1008) <223> RXS03215 <400> 491 atc gat gtt gtc agc gtc gtg gtg gct aac ttc ctg cac cgc gaa atc 48 Ile Asp Val Val Ser Val Val Val Ala Asn Phe Leu His Arg G u lie 1 5 10 gtg gaa gca ctt ctg gca tcc ggc aag cat gtg ctg tgc gag aag cca 96 Val G u Ala Leu Leu Al a Ser G y Lys His Val Leu Cys G u Lys Pro 25 ctg tca gac acc atc gaa gat gca gaa gcc atg att gag gca gcc ggc 144 Leu Ser Asp Thr Ile u Asp Al a u Al a Iet Ile u Ala Al a y 40 cgt gca gca aca aat ggc acc atc gcc cgc atc gga ctg acc tac cgc 192 Arg Ala Ala Thr Asn G y Thr lie Ala Arg lie y Leu Thr Tyr Arg 55 cgt tcc cca ggc gtg gca cac atc cgt gat ctc gtg cag tcc ggc gag 240 Arg Ser Pro G y Val Al a His IIe Arg Asp Leu Val G n Ser G y G u 70 75 ctt ggc aag gtt cta cac gtc acc ggc cac tac tgg acc gac tac gga 288 Leu G y Lys Val Leu His Val Thr G y His Tyr Trp Thr Asp Tyr G y 90 tcc aat gca cag gca cca atc agc tgg cgt tac aag ggg cca aac ggc 336 Ser Asn Ala G n Ala Pro lie Ser Trp Arg Tyr Lys y Pro Asn y 100 105 110 tcc ggc gca ctg gca gat gtg gga agc cac ctc acc tac ctg gca gaa 384 Ser G y Ala Leu Ala Asp Val G y Ser His Leu Thr Tyr Leu Ala G u 115 120 125 ttc gtt gca gga tct gac ttc gcc gcc gtc cgt ggt ggc cag ttg tcc 432 Phe Val Ala y Ser Asp Phe Ala Ala Val Arg Gy Gy G n Leu Ser 130 135 140 acc gtg atc acc gag cgc ccc aag cca ctc ggc gcg att gtc ggc cac 480 Thr Val lie Thr G u Arg Pro Lys Pro Leu G y Ala IIe Val G y His 145 150 155 160 Page 632 Gene Sequence 24-2-06.txt gaa ggc ggc gca gtt tcc gat gaa Su y y Al a Val Ser Asp u tac gaa gca gtg gaa Tyr u Ala Val u aat gat gac Asn Asp Asp 175 at t SIle cag G n gt g Val gaa G u 225 gcc Al a ggc Gy ggc Gy t cc Ser cac Hi s 305 ggc Gy gca t ca Al a Ser gtc agc Val Ser 195 ttc tgt Phe Cys 210 ttc aaa Phe Lys ggc tac G y Tyr ggc ctt G y Leu ttc gtt Phe Val 275 gaa gct G u Al a 290 aat at g Asn IVet gaa acc G u Thr 165 t cc Ser at t SIle aag Lys ttc Phe acc Thr 245 at g bet cag G n agc Ser ct c Leu gcg Al a 325 gga Gy t cc Ser ggc Gy acc Thr 230 at c SIle gat Asp gcg Al a ct g Leu at t SIle 310 gt c Val tcc ttc Ser Phe cag gga G n y 200 tcc gtg Ser Val 215 cca gca Pro Al a acc at c Thr Ile gca cca Ala Pro cgc gca Arg Ala 280 cca cgc Pro Arg 295 gat gct Asp Al a cca gcg Pro Al a at c SIle 185 cac Hi s ct c Leu acc Thr gga Gy ggc Gy 265 ttc Phe tgc Cys gt a Val gct Al a ggt Gy cca Pr o ttt Phe t cc Ser cca Pr o 250 gt g Val ct c Leu gca Al a t ca Ser gct Al a 330 ggc Gy aac Asn gat Asp ggt Gy 235 aag Lys gga Gy gaa G u act Thr cag G n 315 ct g Leu gga Gy acc Thr ttc Phe 220 gac Asp cac Hi s at t SIle gaa G u ttg Leu 300 t ca Ser at c SIle acc Thr ct a Leu 205 cgc Ar g at c SIle cca Pr o ggc Gy at c SIle 285 gaa G u gct Al a cct Pr o gca Al a 190 ggt Gy aac Asn agc Ser t ac Tyr caa Gn 270 gca Al a gaa G u gca Al a gca Al a acc Thr ttt Phe t ca Ser caa G n tgg Tr p 255 aac Asn gga Sy ggg Sy gca Al a aac Asn 335 ct c Leu gaa G u ggc G y gaa G u 240 cgc Ar g gaa Gu at c SIe ct a Leu ggt Sy 320 aac Asn 528 576 624 672 720 768 816 864 912 960 1008 1038 tagaaactat tcagaaagca tcaccatgaa <210> 492 <211> 336 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 492 Ile Asp Val Val Ser Val Val Val Ala 1 5 Val G u Ala Leu Leu Al a Ser y Lys 25 Leu Ser Asp Thr Ile G u Asp Ala G u 40 Arg Ala Ala Thr Asn y Thr lie Ala 55 Arg Ser Pro y Val Al a His I I e Arg 70 Asn Phe Leu His Arg Gu lie 10 His Val Leu Cys G u Lys Pro Ala Net Ile u Ala Ala y Arg lie y Leu Thr Tyr Arg Asp Leu Val n Ser Oy u 75 Page 633 Leu y Lys Val Leu His Val Gene Sequence 24-2-06. t xt Thr Gy His Tyr Trp Thr Asp Tyr G y Ser Ser Phe Thr 145 Su I e G n Val G u 225 Al a Gy Gy Ser Hi s 305 Gy Al a Al a 115 Al a SIle Sy Ser Ser 195 Cys Lys Tyr Leu Val 275 Al a bet Thr Al a Al a Ser G u Val 165 Ser SIle Lys Phe Thr 245 bet G n Ser Leu Al a 325 Pr o Asp Asp Ar g 150 Ser Sy Ser Sy Thr 230 I le Asp Al a Leu SIle 310 Val SIle Val Phe 135 Pr o Asp Ser G n Ser 215 Pr o Thr Al a Ar g Pr o 295 Asp Pr o Ser Sy 120 Al a Lys G u Phe Sy 200 Val Al a SIle Pr o Al a 280 Ar g Al a Al a Tr p 105 Ser Al a Pr o Tyr SIle 185 Hi s Leu Thr Sy Sy 265 Phe Cys Val Al a Ar g Hi s Val Leu G u 170 Sy Pr o Phe Ser Pr o 250 Val Leu Al a Ser Al a 330 Tyr Leu Ar g Sy 155 Al a Sy Asn Asp Sy 235 Lys Sy G u Thr G n 315 Leu Sy Tyr 125 Gy I e Su Thr Leu 205 Ar g SIle Pr o Gy I e 285 G u Al a Pr o Pr o 110 Leu G n Val Asn Al a 190 Gy Asn Ser Tyr G n 270 Al a G u Al a Al a Asn Al a Leu Sy Asp 175 Thr Phe Ser G n Tr p 255 Asn Sy Sy Al a Asn 335 <210> 493 <211> 1031 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (1)..(1008) <223> FRXA01915 <400> 493 atc gat gtt gtc agc gtc gtg gtg gct aac ttc ctg cac cgc gaa atc Ile Asp Val Val Ser Val Val Val Ala Asn Phe Leu His Arg G u lie 1 5 10 gtg gaa gca ctt ctg gca tcc ggc aag cat gtg ctg tgc gag aag cca Page 634 Val G u Ala Leu Leu Al a Ser Gene Sequence 24-2-06. t xt G y Lys His Val Leu Cys G u Lys Pro ct g Leu cgt Ar g cgt Ar g ctt Leu t cc Ser t cc Ser ttc Phe acc Thr 145 gaa G u at t SIle cag G n gt g Val gaa G u 225 gcc Al a ggc gcy ggc Gy gac Asp gca Al a cca Pr o aag Lys gca Al a gca Al a 115 gca Al a at c SIle ggc G y t ca Ser agc Ser 195 tgt Cys aaa Lys t ac Tyr ctt Leu gtt Val 275 at c SIle aat Asn gt g Val ct a Leu gca Al a gca Al a tct Ser gag G u gtt Val 165 t cc Ser at t SIle aag Lys ttc Phe acc Thr 245 at g bet cag G n gaa G u ggc Gy gca Al a 70 cac Hi s cca Pr o gat Asp gac Asp cgc Ar g 150 t cc Ser gga G y t cc Ser ggc Gy acc Thr 230 at c SIle gat Asp gcg Al a gat Asp acc Thr cac Hi s gt c Val at c SIle gt g Val ttc Phe 135 ccc Pr o gat Asp t cc Ser cag G n t cc Ser 215 cca Pr o acc Thr gca Al a cgc Ar g gca gaa Al a G u 40 atc gcc I e Al a at c cgt li e Ar g acc ggc Thr G y agc tgg Ser Trp 105 gga agc G y Ser 120 gcc gcc Al a Al a aag cca Lys Pr o gaa t ac G u Tyr ttc atc Phe Il e 185 gga cac G y Hi s 200 gtg ct c Val Leu gca acc Al a Thr atc gga I e G y cca ggc Pro G y 265 gca ttc Al a Phe 280 gcc Al a cgc Ar g gat Asp cac Hi s 90 cgt Ar g cac Hi s gt c Val ct c Leu gaa G u 170 ggt G y cca Pr o ttt Phe t cc Ser cca Pr o 250 gt g Val ct c Leu at g let at c SIle ct c Leu t ac Tyr t ac Tyr ct c Leu cgt Ar g ggc G y 155 gca Al a ggc G y aac Asn gat Asp ggt Gy 235 aag Lys gga gay gaa G u at t SIle gga Gy gt g Val tgg Tr p aag Lys acc Thr ggt G y 140 gcg Al a gt g Val gga G y acc Thr ttc Phe 220 gac Asp cac Hi s at t SIe gaa G u gag G u ct g Leu cag G n acc Thr ggg G y t ac Tyr 125 ggc G y at t l Ie gaa G u acc Thr ct a Leu 205 cgc Ar g at c SIle cca Pr o ggc G y at c SIle 285 gca Al a acc Thr t cc Ser gac Asp cca Pr o 110 ct g Leu cag G n gt c Val aat Asn gca Al a 190 ggt G y aac Asn agc Ser t ac Tyr caa G n 270 gca Al a gcc Al a t ac Tyr ggc G y t ac Tyr aac Asn gca Al a ttg Leu ggc G y gat Asp 175 acc Thr ttt Phe t ca Ser caa G n tgg Tr p 255 aac Asn gga G y tcc gaa gct gaa agc ctg cca cgc tgc gca act ttg gaa gaa ggg cta Page 635 Gene Sequence 24-2-06.txt Ser G u Ala u Ser Leu Pro Arg Cys Ala Thr Leu G u G u y Leu 290 295 300 cac aat atg cag ctc att gat gct gta tca cag tca gct gca gca ggt His Asn IVet n Leu Ile Asp Ala Val Ser n Ser Ala Ala Ala y 305 310 315 320 ggc gaa acc gtt gcg gtc cca gcg gct gct ctg atc cct gca aac aac Gy G u Thr Val Ala Val Pro Ala Ala Ala Leu lie Pro Ala Asn Asn 325 330 335 tagaaactat tcagaaagca tca <210> 494 <211> 336 <212> PRT <213> Cor ynebact eri um gl ut am cum 960 1008 1031 <400> 494 Ile Asp Val 1 Val Gu Ala Leu Ser Asp Ar g Al a Al a Arg Ser Pro Leu G y Lys Ser Asn Al a Ser G y Al a 115 Phe Val Al a 130 Thr Val I le 145 Su Gy y I le Al a Ser G n Val Ser 195 Val Phe Cys 210 G u Phe Lys 225 Al a G y Tyr Val Ser 5 Leu Leu Thr Ile Thr Asn G y Val Val Leu G n Al a 100 Leu Al a G y Ser Thr G u Al a Val 165 Phe Ser 180 Arg Ile G u Lys Ile Phe Arg Thr 245 Val Val Val Ala Al a Ser G y Lys 25 G u Asp Al a G u 40 Sy Thr Ile Al a 55 Ala His Ile Arg 70 His Val Thr G y Pro Ile Ser Trp 105 Asp Val y Ser 120 Asp Phe Ala Al a 135 Arg Pro Lys Pro 150 Ser Asp G u Tyr Sy Ser Phe lie 185 Ser G n G y Hi s 200 G y Ser Val Leu 215 Thr Pro Ala Thr 230 I e Thr Ile y Phe Val bet l Ie Leu 75 Tyr Tyr Leu Ar g Sy 155 Al a Sy Asn Asp Sy 235 Leu Leu SIle Sy Val Tr p Lys Thr Sy 140 Al a Val Sy Thr Phe 220 Asp Hi s Cys G u Leu G n Thr Sy Tyr 125 Gy l e Su Thr Leu 205 Ar g SIle Ar g G u Al a Thr Ser Asp Pr o 110 Leu G n Val Asn Al a 190 Sy Asn Ser Su lle Lys Pro Al a y Tyr Arg G y G u Tyr G y Asn y Al a u Leu Ser G y Hi s 160 Asp Asp 175 Thr Leu Phe G u Ser G y G n G u 240 Trp Arg 255 Pro Lys His Pro Tyr Page 636 Gy Gy Leu Al a 260 G y Phe Val Phe 275 Ser G u Ala G u 290 His Asn IVet n 305 G y G u Thr Val Gene Sequence 24-2-06.txt IVet Asp Ala Pro Gy Val Gy Ile Gy Gn Asn Gu 265 270 Sn Al a Arg Al a Phe Leu G u G u lie Ala G y lie 280 285 Ser Leu Pro Arg Cys Ala Thr Leu G u G u y Leu 295 300 Leu I e Asp Ala Val Ser G n Ser Al a Ala Ala y 310 315 320 Ala Val Pro Ala Ala Ala Leu lie Pro Ala Asn Asn 325 330 335 <210> 495 <211> 1288 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1258) <223> RXS03224 <400> 495 acgattgtgc tgtcgtttgc gttggtgaat agttctggac tggaactcat tgaacgccgc gcccggctaa ggtgggaggc cgggtatttt gcggcgcaca atg agt ttt gct gaa 11! [et Ser Phe Ala G u cat Hi s at c SIle at t SIle ct g Leu ttc Phe ct g Leu gt c Val ggg Sy at c SIle cct Pr o tgg Tr p ccg Pr o cgc Ar g gag G u aat Asn t ca Ser 120 tgg Tr p gcc Al a gat Asp ttc Phe gac Asp gcg Al a gtt Val gaa G u gt c Val gca Al a gtt Val t cc Ser cgc Ar g cag G n agt Ser cac Hi s ccc Pr o gt c Val gaa G u agc Ser ggc G y ggc G y tct Ser 110 at c SIle ct g Leu 15 aca Thr ct a Leu cac Hi s acc Thr at t SIle 95 aaa Lys ct g Leu ggc Sy cat Hi s ggc Sy ggc G y gag G u gga G y t at Tyr gcg Al a 1 gct Al a cgg Ar g tgc Cys t ac Tyr gaa G u gt g Val ct c Leu gaa G u ttg Leu ct c Leu aac Asn gac Asp gac Asp ctt Leu gac Asp ct c Leu 130 gct Al a aat Asn ct c Leu ct c Leu gac Asp gac Asp 100 acc Thr cac Hi s Page 637 aat ccc gcc gta gtg gat Asn Pro Ala Val Val Asp Gene Sequence 24-2-06.txt ctg gtt gtc gat gtc atg aac cac tgg ctc Leu Val Val Asp Val IVet Asn His Trp Leu gac Asp 150 cct Pr o cac Hi s gt g Val gcc Al a act Thr 230 ttc Phe t ca Ser cca Pr o gat Asp ttt Phe 310 gcg Al a ctt Leu ggt Gy at c SIle 135 cgc gga Arg G y gaa ttt G u Phe gca tgg Al a Tr p aaa agc Lys Ser 200 att tgg lie Trp 215 ttg agt Leu Ser att ggt I e G y aat gcg Asn Ala agc att Ser Ile 280 aac gtt Asn Val 295 tct cca Ser Pro ctg cgc Leu Arg gag att G u Ile gaa gag G u G u 360 ctt gat Leu Asp gca Al a gaa G u 170 gt g Val ggc Gy agc Ser cac Hi s cat Hi s 250 ct g Leu t ac Tyr ggt Gy ggc Gy caa G n 330 aat Asn aca Thr gag G u ggc Gy 155 aaa Lys ggg Gy at t SIle at c SIle aat Asn 235 gac Asp gcc Al a ggc Gy gac Asp acc Thr 315 cac Hi s gaa G u gt g Val aag Lys 140 tgg Tr p gt c Val gag G u gat Asp aaa Lys 220 gaa Gu gt c Val gca Al a gat Asp gat Asp 300 tgg Tr p ccg Pr o gcg Al a cat Hi s cga Ar g ct g Leu at g Net t cc Ser 205 gag G u ttc Phe acc Thr gcg Al a gag G u 285 gcc Al a at t SIle tgg Tr p at g Net ctt Leu 365 ct c Leu cca Pr o at c SIle 190 gtt Val cgc Ar g ct c Leu cgc Ar g at c l Ie 270 cag G n at t l Ie gaa G u ttg Leu acc Thr 350 gat Asp gac Asp gaa G u 175 cat Hi s acc Thr aat Asn gat Asp at t SIle 255 ct c Leu ggc Gy agg Ar g aac Asn t at Tyr 335 t at Tyr ttg Leu gct Al a 160 gt g Val gga Gy gaa G u ttc Phe act Thr 240 gcc Al a ttc Phe ttt Phe cca Pr o at t SIle 320 cag G n aag Lys gaa G u gt c Val cga Ar g gat Asp t ac Tyr ttt Phe 225 ttc Phe acc Thr acg Thr acg Thr cct Pr o 305 t at Tyr gcg Al a t cc Ser gag G u t ac Tyr cga Ar g t ac Tyr gaa G u 210 gaa G u gt a Val cga Ar g gt c Val gga Gy 290 ctt Leu aag Lys cac Hi s gt c Val gt g Val 370 gcc Al a aaa Lys t cc Ser 195 ct g Leu ct c Leu ccg Pr o at c SIle gga Gy 275 ttg Leu cct Pr o gct Al a acc Thr ggt Gy 355 t ct Ser at c l Ie cac Hi s 180 gac Asp tgg Tr p gaa G u cag G n ggt Gy 260 gga Gy aaa Lys gcc Al a ct g Leu gaa G u 340 ctt Leu gtt Val gcc Al a 165 cca Pr o t ac Tyr aaa Lys tgg Tr p aca Thr 245 caa G n acc Thr gag G u gag G u at c SIe 325 gt c Val gga G y cgg Ar g 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1268 1288 gtg ctg ttt cag tac agc gct Val Leu Phe G n Tyr Ser Ala 380 385 t agt t gt cgg 375 ttcaagggta ggggaacaaa <210> 496 Page 638 Gene Sequence 24-2-06.txt <211> 386 <212> PRT <213> Corynebacter i um gl utam cum <400> 496 ebt Ser Phe Ala u His Ala lie Ile 1 Al a Ar g Cys Tyr G u Val Leu G u et 145 Val Ar g Asp Tyr Phe 225 Phe Thr Thr Thr Pr o 305 Gy Pr o Al a Thr bet Phe Leu 115 Asp Hi s Al a Lys Ser 195 Leu Leu Pr o SIle Gy 275 Leu Pr o 5 Pr o Leu vet Asp Al a Gy Thr Thr Leu Al a 165 Pr o Tyr Lys Tr p Thr 245 G n Thr G u G u Ar g Gy Gy Tyr Leu Phe Al a Pr o 135 Ar g G u Al a Lys SIle 215 Leu SIle Asn Ser Asn 295 Ser G u Leu Val SIle Al a Hi s 105 Phe Val SIle Tr p l Ie 185 Ser Ser Ar g Asn SIle 265 Tyr Phe Leu Tr p 10 Al a Asp Phe Asp Al a 90 Val G u Val Al a G u 170 Val Gy Ser Hi s Hi s 250 Leu Tyr Gy Gy Hi s Pr o Tyr G u Pr o 75 Asn Ser Gy Asp Gy 155 Lys Gy I e I e Asn 235 Asp Al a Gy Asp Thr 315 Val Al a Val Ser Ar g G n Ser Hi s Leu 140 Tr p Val G u Asp Lys 220 G u Val Al a Asp Asp 300 Tr p Ty r Pr o Val Val Leu Ar g Ser Asp 125 Val Ar g Leu bet Ser 205 G u Phe Thr Al a G u 285 Al a SIle Leu G y Thr His Leu G y Hi s G y Thr G u Se G y Lys Tyr Leu Al a Asp Val Asp Al a 160 G u Val 175 Hi s G y Thr G u Asn Phe Asp Thr 240 I e Al a 255 Leu Phe G y Phe Arg Pro Asn I e 320 Tyr G n 335 Tyr Lys Ala Leu lie Ala Leu Arg Arg n His Pro Trp Leu 325 330 Page 639 Gene Sequence 24- 2- 06. txt Ala His Thr u Val Leu Gu Ile Ala Asn Gu Ala IVet Thr Tyr Lys 340 345 350 Ser Val y Leu Gy G y G u G u Leu Thr Val His Leu Asp Leu G u 355 360 365 G u Val Ser Val Arg I e Leu Asp Gy G u Lys Val Leu Phe G n Tyr 370 375 380 Ser Al a 385 <210> 497 <211> 1281 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1258) <223> FRXA00038 <400> 497 acgattgtgc tgtcgtttgc gttggtgaat agttctggac cgggtatttt gcggcgcaca tggaactcat tgaacgccgc gcccggctaa ggtgggaggc atg agt ttt gct gaa IVet Ser Phe Ala G u cat Hi s at c l Ie at t SIle ct g Leu ttc Phe ct g Leu gt c Val ggg Gy aat Asn gac Asp at c SIle cct Pr o tgg Tr p ccg Pr o cgc Ar g gag G u aat Asn t ca Ser 120 gcc Al a gga Gy tgg Tr p gcc Al a gat Asp ttc Phe gac Asp gcg Al a gtt Val gaa G u gt g Val gca Al a gt c Val gca Al a gtt Val t cc Ser cgc Ar g cag G n agt Ser cac Hi s ct g Leu 140 tgg Tr p ccc Pr o gt c Val gaa Gu agc Ser ggc Gy ggc Gy tct Ser 110 at c SIle gt c Val ct c Leu ct g Leu 15 aca Thr ct a Leu cac Hi s acc Thr at t SIle 95 aaa Lys ct g Leu gat Asp ggc Gy cat Hi s ggc Gy ggc Gy gag G u 80 gga Gy t at Tyr gcg Al a gt c Val 1 gct Al a cgg Ar g tgc Cys t ac Tyr gaa G u gt g Val ct c Leu gaa G u at g 1et 145 ttg Leu ct c Leu aac Asn gac Asp gac Asp ctt Leu gac Asp ct c Leu 130 aac Asn gct Al a aat Asn ct c Leu ct c Leu gac Asp gac Asp 100 acc Thr cac Hi s tgg Tr p ccc Pr o ct a Leu at g bet gat Asp gcg Al a ggc Gy acc Thr acg Thr ct c Leu gac gct gtc tac gcc atc gcc Asp Ala Val Tyr Ala lle Ala Page 640 Gene Sequence 24-2-06.txt cct gaa ttt tgg Pro G u Phe Tr p cac gca tgg at c His Ala Trp Ile 185 gtg aaa agc t cc Val Lys Ser Ser 200 gcc att tgg agc Ala lie Trp Ser 215 act ttg agt cgc Thr Leu Ser Arg 230 ttc att ggt aac Phe Ile G y Asn tca aat gcg atc Ser Asn Ala lle 265 cca agc att tac Pro Ser Ile Tyr 280 gat aac gtt ttc Asp Asn Val Phe 295 ttt tct cca ctg Phe Ser Pro Leu 310 gcg ctg cgc agg Ala Leu Arg Arg ctt gag att gct Leu G u Ile Al a 345 ggt gaa gag ctg G y G u G u Leu 360 atc ctt gat ggc Ile Leu Asp G y 375 ttcaagggta ggg gaa G u 170 gt g Val ggc Sy agc Ser cac Hi s cat Hi s 250 ct g Leu t ac Tyr ggt Sy ggc Sy caa G n 330 aat Asn aca Thr aaa Lys ggg Sy at t SIle at c SIle aat Asn 235 gac Asp gcc Al a ggc G y gac Asp acc Thr 315 cac Hi s gaa G u gt g Val gt c Val gag G u gat Asp aaa Lys 220 gaa G u gt c Val gca Al a gat Asp gat Asp 300 tgg Tr p ccg Pr o gcg Al a cat Hi s ct g Leu at g Met t cc Ser 205 gag G u ttc Phe acc Thr gcg Al a gag G u 285 gcc Al a at t SIle tgg Tr p at g et ctt Leu 365 cca Pr o at c SIle 190 gtt Val cgc Ar g ct c Leu cgc Ar g at c l Ie 270 cag G n at t l Ie gaa G u ttg Leu acc Thr 350 gat Asp gaa G u 175 cat Hi s acc Thr aat Asn gat Asp at t SIle 255 ct c Leu ggc G y agg Ar g aac Asn t at Tyr 335 t at Tyr ttg Leu 160 gt g Val gga Sy gaa G u ttc Phe act Thr 240 gcc Al a ttc Phe ttt Phe cca Pr o at t SIle 320 cag G n aag Lys gaa G u cga Ar g gat Asp t ac Tyr ttt Phe 225 ttc Phe acc Thr acg Thr acg Thr cct Pr o 305 t at Tyr gcg Al a t cc Ser gag G u cga Ar g t ac Tyr gaa G u 210 gaa G u gt a Val cga Ar g gt c Val gga G y 290 ctt Leu aag Lys cac Hi s gt c Val gt g Val 370 aaa Lys t cc Ser 195 ct g Leu ct c Leu ccg Pr o at c SIle gga G y 275 ttg Leu cct Pr o gct Al a acc Thr ggt Sy 355 t ct Ser cac Hi s 180 gac Asp tgg Tr p gaa G u cag G n ggt Sy 260 gga G y aaa Lys gcc Al a ct g Leu gaa G u 340 ctt Leu gtt Val cca Pr o t ac Tyr aaa Lys tgg Tr p aca Thr 245 caa G n acc Thr gag G u gag G u at c lle 325 gt c Val gga Sy cgg Ar g 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1268 1281 gag aag gtg G u Lys Val 380 ctg ttt cag tac Leu Phe G n Tyr agc gct Ser Al a 385 t agt t gt cgg <210> 498 <211> 386 <212> PRT <213> Corynebacter i um gl utam cum <400> 498 IVet Ser Phe Ala u His Ala IIe IIe Trp His Val Tyr Pro Leu y Page 641 Gene Sequence 24-2-06.txt Al a Ar g Cys Tyr G u Val Leu G u Met 145 Val Ar g Asp Tyr Phe 225 Phe Thr Thr Thr Pr o 305 Tyr Al a Ser Gy Pr o Al a Thr vet Phe Leu 115 Asp Hi s Al a Lys Ser 195 Leu Leu Pr o SIle Gy 275 Leu Pr o Al a Thr Gy 355 Pr o Leu et Asp Al a Gy Thr Thr Leu Al a 165 Pr o Tyr Lys Tr p Thr 245 G n Thr G u Su l e 325 Val Gy I e I e Leu Phe 70 Leu Val Gy Asn Asp 150 Pr o Hi s Val Al a Thr 230 Phe Ser Pr o Asp Phe 310 Al a Leu Gy Ar g Gy Gy Tyr Leu Phe Al a Pr o 135 Ar g G u Al a Lys l Ie 215 Leu SIle Asn Ser Asn 295 Ser Leu G u G u Pro G u Ala Pro Trp Leu Asp Tyr 40 Pro Val Phe G u Arg lie Asp Pro 75 G u Ala Ala Asn 90 Asn His Val Ser 105 Ser Phe G u y 120 Ala Val Val Asp G y Ile Al a G y 155 Phe Trp G u Lys 170 Trp Ile Val y 185 Ser Ser G y Ile 200 Trp Ser Ser Ile Ser Arg His Asn 235 G y Asn Hi s Asp 250 Ala lie Leu Ala 265 I e Tyr Tyr G y 280 Val Phe Gy Asp Pro Leu y Thr 315 Arg Arg G n His 330 Ile Ala Asn G u 345 G u Leu Thr Val 360 Ala Pro Val Ser Ar g G n Ser Hi s Leu 140 Tr p Val G u Asp Lys 220 G u Val Al a Asp Asp 300 Tr p Pr o Al a Val Val Leu Ar g Ser Asp 125 Val Ar g Leu vet Ser 205 G u Phe Thr Al a G u 285 Al a SIle Tr p vet Thr His Leu G y Hi s G y Thr G u Se G y Lys Tyr Leu Al a Asp Val Asp Al a 160 G u Val 175 Hi s G y Thr G u Asn Phe Asp Thr 240 I e Al a 255 Leu Phe G y Phe Arg Pro Asn I e 320 Tyr G n 335 Tyr Lys His Leu Asp Leu G u 365 Page 642 ene Sequence 24- 2- 06. txt (31u Val Ser Val Ar g I Ie Leu Asp G31y O31u Lys Val Leu Phe O31n Tyr 370 375 380 Se r Al a 385 <210O> <211 <212> <213> <220> <221 <222> <223> 499 517 DNA Cor ynebact er i urn gi ut ani curn ODS 101). (517) RXCO 0233 <400> 499 cgcct ccagc agt tgaggga gaagttccaa cacttgcacc aactgaggaa gcaactgtgc aatagcgctt tagacacaga ctcatgacag aatagaagac at g vb t 1 agt gt g aat Ser Val Asn gca gat ct g aac Al a Asp Leu Asn gct Al a gt c gaa gag caa Val1 G u Gu Gn gga agg gcc cca G y Ar g Al a Pr o cga ggt Ar g G y gt c ct c gat Val Leu Asp at g acc gca l'bt Thr Al a tct tac cgc agc Ser Tyr Arg Ser cct Pr o gat gga gt a ccc Asp G31y VaI Pr o ggt gt g gt g G31y Val Val acc ct g t ac Thr Leu Tyr cca aaa ct g gat Pro Lys Leu Asp gga acc cca ttc Gy Thr Pro Phe cca Pr o t ac tt g Tyr Leu aca gat cca cgc Thr Asp Pro Arg ct g Le u acc acc gag gca Th r Th r 03 u Al a cgc ct c gag gt c Ar g Leu G31u Val gca Al a t tg gt a at g aag Leu Val l'bt Lys at g act gat cgc l'bt Thr Asp Arg ct t Le u t cc acc gac gaa Ser Thr Asp 03 u ctt cgt gcc gac Leu Arg Ala Asp t ac Tyr cag cgc gcc cac O31n Ar g Al a Hi s cac t tc ct g gca Hi s Phe Leu Al a aag cgc Lys Ar g 1 00 aac gca att As n Al a I I e gac cga gt g Asp Ar g Val 120 gaa gat ct c ggc acg gat t tt t cc ggc ggt ggc at g cct Ou Asp Leu Gy Thr Asp Phe Ser Gy Gy Gy l'bt Pro 105 110 115 aag t gc ct t cac Lys Cys Leu Hi s ct c at t gac t at Leu I I e Asp Tyr gca Al a 130 ct g gca gaa Leu Al a 03 u ggc cca G31y Pro 135 cac cat t tc ct t Hi s Hi s Phe Leu <210O> 500 <211> 139 <21 2> PRT <21 3> Cor ynebact er i urn gI ut anri curn <400> 500 l'bt Ser Val Asn (31u Al a Asp Leu Asn Al a Val (31u 31u O31n Leu G31y Page 643 Gene Sequence 24 1 5 10 Arg Ala Pro Arg Gy Val Leu Asp lie Ser Tyr 25 Val Pro y Val Val ebt Thr Ala Pro Lys Leu 40 Phe Pro Thr Leu Tyr Tyr Leu Thr Asp Pro Arg 55 Ser Arg Leu G u Val Al a Leu Val IVet Lys Trp 70 75 Ser Thr Asp G u G u Leu Arg Ala Asp Tyr G n 90 Phe Leu Ala Lys Arg Asn Ala lie G u Asp Leu 100 105 G y G y G y Iet Pro Asp Arg Val Lys Cys Leu 115 120 Tyr Ala Leu Ala G u G y Pro His His Phe Leu 130 135 <210> 501 <211> 849 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(826) <223> RXC00236 <400> 501 aatgcgagag ttctaaaacg agccggtaac atcgaccccc agcaatggga tttggatgcg gttcggtttt ggccgtcatc -2-06. txt Arg Ser Pro Asp Asp G y Leu Thr Thr IVet Thr Asp Arg Ala Hi s G y Thr Asp 110 Hi s Val Leu 125 Asp Thr G u Ar g G u Phe SIle Gy Pr o Al a Leu Hi s Ser Asp gtt Val ct c Leu ccg Pr o ttt Phe at t SIle ggc Gy tgg Tr p caa G n at t SIle gcg Al a gct Al a aat Asn ct c Leu cct Pr o aca Thr cca Pr o gga Gy ggg Gy agc Ser gaa G u gag G u at t SIle aac Asn 75 tgg Tr p ttc Phe gat Asp gca Al a gct Al a gca Al a aat Asn gat Asp ccg Pr o gga Gy gat Asp ct c Leu ttg Leu gga acg G y Thr 15 ccg gcg Pro Al a cgc aca Arg Thr act ggt Thr G y atc gcg I e Al a 80 gca ggt Al a G y 95 Page 644 atgagttcag gggttagaaa atg gtg atc tca ttt et Val lie Ser Phe 1 gca cct att cgc caa Ala Pro lie Arg n cgt ggt gta gaa gtt Arg y Val G u Val tcc aac cat ttg cgt Ser Asn His Leu Arg gtg tcc gct caa gcg Val Ser Ala G n Ala agt act gcg tgg cct Ser Thr Ala Trp Pro atc ggc cag gtg gaa Ile y n Val u 100 Gene Sequence 24-2-06.txt acc Thr gat Asp ggt Sy gat Asp 150 ccg Pr o gcg Al a ttg Leu gct Al a gat Asp 230 cac ggt Hi s G y 105 aat gga Asn y 120 ccg ggg Pro G y gat act Asp Thr acg tgg Thr Trp ccc aac Pro Asn 185 t cc aac Ser Asn 200 cat tct Hi s Ser gcc gcg Al a Al a acc tac aac Thr Tyr Asn gtt Val ttt Phe gaa G u cga Ar g 170 caa G n gat Asp t ac Tyr gca Al a gcg Al a t at Tyr 155 ctt Leu at t SIle cgt Ar g aac Asn acc Thr gaa G u 140 gat Asp at g Met gat Asp gac Asp at g bet 220 ggc aaa atg G y Lys Ibet 110 cct cca at c Pro Pro Ile 125 att ccc gac lie Pro Asp cgc gcg gta Ar g Al a Val gga ttg gat G y Leu Asp 175 gac gca gca Asp Al a Al a 190 ttg tcc act Leu Ser Thr 205 t ct aat cag Ser Asn G n ttc ggg ggc agt tcc ctg Phe y y Ser Ser Leu 115 at c SIle act Thr ggt G y 160 gca Al a ttg Leu cct Pr o t at Tyr ggc Gy gat Asp 145 ccc Pr o aac Asn agt Ser gaa Gu ttg Leu 225 gtt Val 130 ggt G y at g Net ggt G y gcc Al a gga Gy 210 at g bet cca Pr o ggg G y cag G n gat Asp gca Al a 195 tgg Tr p gac Asp ctt Leu gaa G u ttc Phe ggg G y 180 aac Asn acc Thr gtt Val gat Asp tta Leu att SIe 165 gta Val ctg Leu gca Al a cga Ar g t cc tac Ser Tyr 235 gct tta cga cag ccg gcg atc taaaacttaa Ala Leu Arg n Pro Ala lie 240 caagcgcaac ccc <210> 502 <211> 242 <212> PRT <213> Corynebacter i um gl utam cum <400> 502 Net Val lie Ser Phe Val y Trp Al a 1 5 Ala Pro lie Arg n Leu Gn n lie Arg y Val u Val Pro On Ile Asp 40 Ser Asn His Leu Arg Phe Trp Ala G u 55 Val Ser Ala G n Ala Ile Ala Ala Tyr 70 Ser Thr Ala Trp Pro G y Cys Asn Leu IIe G y G n Val u Thr Arg His G y 100 105 G y G y Ser Ser Leu Asp G u Asn G y 115 120 Leu Ser Phe 10 Pro u Asp Thr G u Al a Pro li e Al a G y Asn Al a 75 Sy Trp Asn 90 Thr Tyr Asn Val Al a Thr Page 645 bet Val Asp G n G u Thr Sy Pr o 125 Sy Pr o Ar g Thr SIle Al a Iet I le Thr Al a Thr Sy Al a Sy Phe SIe Gy Asp 145 Pr o Asn Ser G u Leu 225 Al a Pr o Sy G n Asp Al a 195 Tr p Asp Asp Leu SIle 165 Val Leu Al a Ar g Gy Asp 150 Pr o Al a Leu Al a Asp 230 Ser 135 Gy G u Asp Cys Val 215 Al a Gene Sequence 24-2-06.txt Pro Gy Phe Ala Gu Ile Pro 140 Asp Thr G u Tyr Asp Arg Ala 155 Thr Trp Arg Leu Iet G y Leu 170 Pro Asn n Ile Asp Asp Ala 185 190 Ser Asn Asp Arg Asp Leu Ser 200 205 His Ser Tyr Asn IVet Ser Asn 220 Ala Ala Ser Tyr Ala Leu Arg 235 Asp Val Asp 175 Al a Thr G n G n <210> 503 <211> 1113 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1090) <223> RXC00271 <400> 503 tagtttaaat catgagacat ttcacatatg gttctttatc tctgcccctt tttgaaaata acactttaag gagatgtgcc cgagacatgt gt t gacgctg tcg Ser tcg Ser ggt G y gca Al a ggc Gy act Thr gt a Val t ca Ser gac Asp gat Asp gac Asp ct g Leu gca Al a gat Asp t ac Tyr gcg Al a acc Thr at t SIle at c l Ie agt Ser gtt Val act Thr gat Asp cag G n act Thr agc Ser at c SIle ttc Phe caa Gn ttc Phe gtt Val 15 t cc Ser aac Asn aag Lys aac Asn gct Al a 95 ggc G y acc Thr cag G n gaa G u gct Al a 80 tct Ser ttt tct tcc cgt Phe Ser Ser Arg ttg gcg ttg gct Leu Ala Leu Ala act gat gct gca Thr Asp Ala Ala gtt cag cac cct Val G n His Pro ttt gaa gag gca Phe G u G u Al a ggc gag cag ggc Gy Gu On Gy aat ttg gat ctc Asn Leu Asp Leu 100 gtg ttg gct gtt gca act cca gca gca cag gca act gcg cag aat atc Page 646 Val Leu Ala Val Ala Thr 105 Gene Sequence 24- 2- 06. txt Pro Ala Ala n Ala Thr Ala n Asn Ile 110 115 act Thr gag G u tct Ser 150 gtt Val aac Asn ggg G y cag G n gac Asp 230 cag G n ggc G y act Thr acc Thr gat Asp 310 aag Lys at c SIle 120 gt g Val at c SIle gac Asp cag G n t cc Ser 200 gtt Val at g bet cag G n ct g Leu gag G u 280 cct Pr o gca Al a gaa Gu cca gt c Pro Val gat tct Asp Ser gca ccg Ala Pro gca aag Al a Lys 170 gtg cag Val G n 185 gtt aat Val Asn gaa gct G u Al a gtt gtt Val Val at c cct Ile Pro 250 gca acc Al a Thr 265 atg gct [et Al a gtg gag Val G u gag cgc G u Arg cgc gta Arg Val 330 ttc Phe gaa G u 140 gag G u at c lile gat Asp cag G n ggc G y 220 ggt G y at c SIle ggt G y cgt Ar g gca Al a 300 ggc G y acc Thr 125 gca Al a cag G n ggc G y gag G u act Thr 205 gat Asp at t SIle ggc G y at c SIle at t SIle 285 act Thr gt g Val gca gtt Al a Val cct ggc Pro G y cag ttg G n Leu at c gt c Ile Val 175 gtc acc Val Thr 190 gt c act Val Thr gtt gat Val Asp tct tct Ser Ser gct gag Al a u 255 gat tac Asp Tyr 270 ctg cag Leu G n gag ttc G u Phe gag at c G u Ile acc Thr gga G y gag G u 160 t ac Tyr aag Lys acc Thr gt c Val ct g Leu 240 t cc Ser acc Thr gac Asp acc Thr cct Pr o 320 gat gca Asp Al a 130 aac gt c Asn Val 145 ctt ttg Leu Leu gcg tct Al a Ser gct gct Al a Al a gtg aac Val Asn 210 atc tac Ile Tyr 225 gtt cag Val G n ggc act G y Thr gag ctt G u Leu ggc gaa G y G u 290 tac gtg Tyr Val 305 caa gag G n u tcg Ser ggt G y cag G n gag G u 180 cca Pr o at t SIle cca Pr o gct Al a gag G u 260 cgc Ar g cca Pr o aac Asn ttg Leu gca Al a act Thr ct g Leu 165 gt c Val ct g Leu cag G n act Thr gag G u 245 ggt G y cag G n gca Al a gaa G u gat Asp 325 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1113 tgatcggcgc ttttgagttc gga <210> 504 <211> 330 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 504 Iet Phe Ser Ser Arg Ser Lys Val Leu Ala Ser lie Phe Thr Val G y 1 5 10 Page 647 Gene Sequence 24-2-06.txt Ala Leu Ala Leu Ala Ser Cys Ser Ser Asp Ser Ser Asp Ser Ser Thr Ser Leu Al a Asn Asp Thr Asp Asn 145 Leu Al a Al a Val SIle 225 Val Sy G u Sy Tyr 305 G n Asp G n G u G u Leu G n 115 Val Thr G n Sy G u 195 G u Val Val Val Sy 275 Asp SIle SIle Al a Pr o Al a Sy Leu SIle Al a Thr Leu 165 Val Leu G n Thr G u 245 Gy G n Al a G u Asp 325 Sy Al a Sy 70 Thr Val Thr G u Ser 150 Val Asn Sy G n Asp 230 G n Sy Thr Thr Asp 310 Lys Sy Leu Val Al a Leu Asp Leu 135 Asp Pr o Ser Leu Al a 215 Asn Lys Al a Sy et 295 Al a Al a Asp 40 Asp Asp Leu Al a SIle 120 Val SIle Asp G n Ser 200 Val let G n Leu G u 280 Pr o Al a G u Ser Al a Val Thr Val 105 Pr o Asp Al a Al a Val 185 Val G u Val SIle Al a 265 let Val G u Ar g Ar g Thr Phe Ser Thr Leu Asn l Ie 155 Ser Val Thr Leu Ser 235 Val Leu Leu Thr G n 315 Val Thr Asp G n Pr o Phe G u 140 G u I e Asp G n Sy 220 Gy I e Gy Ar g Al a 300 Sy Sy Sy G u G n Al a Thr 125 Al a G n Sy G u Thr 205 Asp SIle Gy I e I e 285 Thr Val SIle Phe G n Phe Al a 110 Al a Pr o G n SIle Val 190 Val Val Ser Al a Asp 270 Leu G u G u Asn Lys Asn Al a G n Val Sy Leu Val 175 Thr Thr Asp Ser G u 255 Tyr G n Phe SIe <210> 505 <211> 1263 <212> DNA <213> Corynebacteri um gl utam cum <220> Page 648 Gene Sequence 24-2-06.txt <221> CDS <222> (101)..(1240) <223> RXC00338 <400> 505 tcttagaagg cgtagtcaca ccattaacct tgccagaatt tgggaaacga acatgcggta ccaaccaggg gagttaatgc gtt Val gcg Al a tcg Ser gac Asp ttg Leu aag Lys cac Hi s gag G u ggc G y t ac Tyr 150 t ac Tyr gt g Val t cc Ser cgg Ar g gat Asp agc Ser aag Lys gct Al a ct g Leu at t SIle aat Asn gt c Val 120 at g bet aag Lys gag G u ggg Gy gag G u 200 acg Thr cgc Ar g gac Asp gt c Val gac Asp ggg G y acc Thr gat Asp at c SIle aaa Lys at g bet gcc Al a 170 aat Asn cgc Ar g gt g Val cgc Ar g aaa Lys ggt Gy at g bet gt g Val 75 ct a Leu t cc Ser acg Thr tgg Tr p ctt Leu 155 ttt Phe ccg Pr o at c SIle ct g Leu at t SIle gt g Val tta Leu ggt Gy acg Thr at t SIle gcg Al a gcc Al a ggc G y 140 ttc Phe gag G u gca Al a gag G u cgg Ar g 220 ttg Leu ggg Gy gca Al a 45 ttg Leu tct Ser cgc Ar g cgc Ar g ggg G y 125 gt g Val gac Asp at c SIle gag G u ttt Phe 205 gag G u gat Asp ct g Leu ct c Leu gac Asp gtt Val ggc G y cca Pr o 110 cga Ar g cac Hi s gca Al a gaa G u ggg Gy 190 gtt Val gct Al a gct Al a tgc Cys tgc Cys ct g Leu gcg Al a 80 gt g Val gt c Val at c SIle ct g Leu gcc Al a 160 acc Thr gt c Val ccg Pr o ccg Pr o tttcaaggct tggctagact gtg agt gat gta acc 11! Val Ser Asp Val Thr 1 tat ccg ccg gcg ttg 16 Tyr Pro Pro Ala Leu ggt gat cca aca gag 21 Sy Asp Pro Thr u acc cag gca gtg gcc Thr G n Ala Val Ala atc att cac cac cca Ile Ile His His Pro gat gag cca aaa ggc Asp G u Pro Lys Gy gca ctg ttt tcc gcg Ala Leu Phe Ser Ala 100 aac gat aaa ctc gcc Asn Asp Lys Leu Ala 115 gcg aca cgg ctt tta 49! Ala Thr Arg Leu Leu 130 ccc aag gat gca gcg 54 Pro Lys Asp Ala Ala 145 ggt gcg atc ggc gac 59! G y Al a lle G y Asp 165 ggg cag ttt agg ccc 64; Gy G n Phe Arg Pro 180 gat aag ctt ttt aaa 69 Asp Lys Leu Phe Lys 195 cgc aac ctg cgc gcc 73! Arg Asn Leu Arg Ala 210 tat gag gag cct gcc 78 Tyr G u G u Pro Ala 225 tta gaa aat gcg acc 83! ttc gat att gtt gaa atg cac agc gct gag agt Page 649 Phe Asp Ile Val G u Mebt 230 235 Gene Sequence 24-2-06.txt His Ser Ala u Ser Leu G u Asn Ala Thr 240 245 gga ttg ggt cgt gtg ggt gaa ttg ccg Sy Leu G y Arg Val G y G u Leu Pro 250 ttc gtg caa caa gtg gcc aac aac ctg Phe Val n G n Val Ala Asn Asn Leu 265 270 cgc gct acc ggc gat cct gaa caa atg Arg Al a Thr G y Asp Pro G u G n IVet 280 285 tca ggg tcg ggt gac agt ttc tta aac Ser y Ser y Asp Ser Phe Leu Asn 295 300 gac gtt tat gtc act tct gat ctg cgc Asp Val Tyr Val Thr Ser Asp Leu Arg 310 315 ctc cga gaa ggt ggc cct gca gta atc Leu Arg G u G y G y Pro Ala Val lie 330 gaa ttt cca tgg act tcc caa gcc caa G u Phe Pro Trp Thr Ser G n Ala G n 345 350 cca cag gtt gaa gtt gat gtg att tcg Pro G n Val G u Val Asp Val lie Ser 360 365 atg tct gcg cga gca gtg aac taaattcl IVet Ser Ala Arg Ala Val Asn 375 380 <210> 506 <211> 380 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 506 Val Ser Asp Val Thr Val y Asp Ile 1 5 Tyr Pro Pro Ala Leu Al a u Ser Trp Sy Asp Pro Thr u Ser Val Lys Arg 40 Thr G n Ala Val Ala Asp Lys Ala Val 55 Ile Ile His His Pro Leu Leu Leu Arg 70 Asp u Pro Lys y Lys Val Ile His Ala Leu Phe Ser Ala His Thr Asn Al a 100 105 Asn Asp Lys Leu Ala G u Leu Val y gag G u 255 cct Pr o gt g Val gat Asp cac Hi s gat Asp 335 gaa G u at c SIle ccg Pr o gt c Val t cc Ser gt g Val cat Hi s 320 act Thr at t SIle cgc Ar g at g et acc Thr cgt Ar g at t SIle 305 cca Pr o gca Al a ttg Leu aca Thr cgc Ar g gaa G u gt g Val 290 aag Lys gtt Val cac Hi s cag G n gac Asp 370 gcg Al a 260 ggc G y gtt Val gga G y gaa G u gcc Al a 340 aaa Lys tgg Tr p 883 931 979 1027 1075 1123 1171 1219 1263 :tg agaactaaaa aag Arg Arg Il e 10 Asp Lys Val Val G y Leu Asp Iet y G y Val Thr 75 Thr Leu Ile 90 Asp Ser Al a I le Thr Al a Page 650 Leu Sy Al a Leu Ser Ar g Ar g Sy G u I e Asp vet Al a Sy G y Pr o Al a Cys Cys Leu Al a Val Val SIe Gene Sequence 24-2-06.txt 125 Val His Val Leu Al a Pr o 145 Gy Gy Asp Ar g Tyr 225 Leu bet Thr Ar g SIle 305 Pr o Al a Leu Thr Ar g Asp SIle Phe Leu 195 Leu G u Asn Leu Tr p 275 Al a Leu Asp Tr p Asp 355 Pr o Leu Al a Asp 165 Pr o Lys Al a Al a Thr 245 Asp Val Ser Val Tyr 325 Ser Al a Gy 135 Leu Ar g G u Leu Leu 215 Asp Leu Val Al a Gy 295 Val Ar g Phe G n Ser 375 Asp Lys Trp Lys Cys Al a 185 Leu Ser Val Ar g G n 265 Gy Gy Val Gy Tr p 345 G u Net Al a 170 Asn Ar g Val G u Val 250 Val Asp Asp Thr Gy 330 Thr Val Leu 155 Phe Pr o SIle Leu bet 235 Gy Al a Pr o Ser Ser 315 Pr o Ser Asp Gy 140 Phe G u Al a G u Ar g 220 Hi s G u Asn G u Phe 300 Asp Al a G n Val Asp SIle G u Phe 205 G u Ser Leu Asn G n 285 Leu Leu Val Al a SIle 365 Al a G u Gy 190 Val Al a Al a Pr o Leu 270 Met Asn Ar g SIle G n 350 Ser Gy Gy 175 Asp Al a Hi s G u G u 255 Pr o Val Asp Hi s Asp 335 G u lie Trp Thr bet Ala Arg Ala Val <210> 507 <211> 1470 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1447) <223> RXC00362 <400> 507 cactttttgg gtgaaaattc cacgaagtta atgccgcttt aagtcaattc aatcacatgt aacatgctac ggttttttcg gtcacttaaa ggaggcgctt atg gga atc att gct 115 NIet Gy Ile Ile Ala 1 Page 651 Gene Sequence 24-2-06.txt ctg ctc gtt ttt atc gca att gcc gtg Leu Leu Val Phe Ile Ala lle Ala Val ata ttg aat Ile Leu Asn gtg ttt ttg aaa Val Phe Leu Lys cga Ar g gt c Val gct Al a ggc Gy ctt Leu act Thr aac Asn gga Gy ctt Leu 150 ttg Leu gct Al a t ac Tyr gt g Val ttc Phe 230 gaa G u tcg Ser at t SIle ggt Gy cag G n gtt Val tcg Ser gga Gy gcg Al a 120 act Thr gtt Val gct Al a ggt Gy aca Thr 200 ttc Phe gga Gy tta Leu at c SIle gaa G u aat Asn gaa G u caa G n ttt Phe gcg Al a gtt Val gaa G u ttg Leu gca Al a 170 gcg Al a aaa Lys gag G u ttg Leu aca Thr 250 tgg Tr p gca Al a gca Al a gtt Val t ca Ser 75 ggt Gy ct c Leu ggc Gy agg Ar g cct Pr o 155 gct Al a t at Ty r gat Asp gca Al a at c SIle 235 cat Hi s gt g Val ttg Leu ccg Pr o act Thr act Thr cgg Ar g at t SIle t ca Ser t cc Ser 140 ccc Pr o tct Ser gca Al a aaa Lys at g Met 220 ccc Pr o gga Gy cca Pr o ct a Leu aca Thr 45 ttc Phe gga Gy ctt Leu gga Gy gt g Val 125 gca Al a aat Asn tcg Ser gt g Val at t SIle 205 aag Lys gt a Val gtt Val at t SIle gtt Val tta Leu gca Al a ttg Leu cga Ar g ct c Leu 110 acg Thr acg Thr t ca Ser gcc Al a ct c Leu 190 cct Pr o act Thr at c SIle ggg Gy ct g Leu 270 gga Gy ct g Leu ggt Gy at t SIle ggt Gy 95 at c SIle at c SIle tta Leu aca Thr tct Ser 175 t ac Tyr gcc Al a gga Gy ct c Leu gag G u 255 at t SIle tta Leu at t SIle at g bet gat Asp 80 ggc Gy gct Al a cct Pr o gt c Val at g bet 160 cag G n cgc Ar g acc Thr tgg Tr p aca Thr 240 tct Ser acg Thr gt a Val gat Asp gcc Al a cga Ar g gca Al a gga Gy tgg Tr p gcg Al a 145 ttc Phe gt g Val tta Leu cct Pr o cgt Ar g 225 at c SIle ggt Gy gca Al a gga Gy gct Al a ttt Phe tta Leu ggt Gy t ca Ser at g Net 130 ggc Gy at c SIle t ac Tyr gcg Al a gat Asp 210 t ca Ser ggc Gy gtt Val at t l Ie act Thr gt a Val gtt Val at c SIle t at Tyr acg Thr 115 aaa Lys aac Asn at t SIle at t SIle gt c Val 195 gat Asp ccg Pr o cca Pr o aaa Lys gct Al a 275 gcg Al a gt g Val ttc Phe gca Al a gtt Val 100 gct Al a aag Lys tct Ser ttg Leu gct Al a 180 gt c Val caa G n ttg Leu ttg Leu tcg Ser 260 ct g Leu ctt Leu gat Asp at g bet at c SIe t cc Ser gga Gy acg Thr ggc Gy gca Al a 165 ttg Leu ttt Phe cgg Ar g at c SIe tct Ser 245 at g bet att SIe Page 652 Gene Sequence 24-2-06.txt gaa ggg cgt G u G y Ar g 280 at c t cc aag Ile Ser Lys 295 tct gcg ctt Ser Al a Leu 310 cag ttg tct 0 n Leu Ser gtg atc att Val Ile lle tca aca gca Ser Thr Al a 360 gct gcg gt a Al a Al a Val 375 tgc act t cc Cys Thr Ser 390 ctt tct gct Leu Ser Al a ctg att acg Leu Ile Thr gga atg gga Sy Met y 440 aaa cga att gct aac aac atg gca Lys Arg lie Ala Asn Asn IVet Ala cac ttt agg Hi s Phe Ar g gtt cag Val n ttg Leu gca Al a tgg Tr p 330 tgc Cys gcg Al a at t SIle gaa G u at c l Ie 410 ttt Phe cca Pr o gcg Al a 315 ctt Leu at c SIle gct Al a gat Asp ggt Gy 395 gcc Al a gtt Val caa G n 300 aac Asn gat Asp at g bet gcg Al a cca Pr o 380 gca Al a gat Asp gt c Val gcc Al a at g bet at g Net at t SI e 350 ggt Gy gt g Val ccg Pr o aac Asn at g 4et 430 acc Thr gaa Gu gac Asp 335 gt g Val gct Al a gcg Al a ccg Pr o cca Pr o 415 at t SI e gt a Val gaa Gu 320 ct a Leu gt g Val ccc Pr o at c l Ie gt g Val 400 acg Thr ct g Leu gga Gy 305 ct g Leu cct Pr o gca Al a gct Al a gt a Val 385 ggc Gy aaa Lys ctt Leu 290 at t SIle ggt Gy aag Lys acg Thr gt c Val 370 gt g Val gcg Al a at g Net gct Al a tcg Ser gtt Val t ct Ser cca Pr o 355 gct Al a at c SIle ccg Pr o ttc Phe tgg Tr p 435 ttg Leu ggc Gy gt c Val 340 ct g Leu gcg Al a ttg Leu at t SIe gt a Val 420 ct a Leu 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1457 1470 tta cca gt g Leu Pro Val gtt cct acg ggt Val Pro Thr Gy t aaaggggt a aaaat gaact caa <210> 508 <211> 449 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 508 IVet Gy lie lie Ala Leu Leu Val Phe 1 5 Asn Val Phe Leu Lys Arg Asp lie Ser 25 Val y Thr Ala Leu Val Gy y Val 40 Asp Ala Val Val Asp Al a Ala G n Ser 55 Ala Phe Val Phe Net G y Ile Val Val 70 Ile Ala lle Ala Val Ile Leu 10 G u Ala Leu Leu Val y Leu Asn Ala Pro Thr Leu Leu Ile G u Val Thr Phe Ala Gy bet G n Ser Thr 0 y Leu Ile Asp 75 Page 653 Ar g Al a Gy Tr p Al a 145 Phe Val Leu Pr o Ar g 225 I le Gy Al a Hi s Gy 305 Leu Pr o Al a Al a Val 385 Gy Lys Leu Leu lie Ala lie Leu Asn Gene Sequence 24-2-06.txt Ser lie Phe Gy Arg Leu Arg Gy Gy 90 Tyr Thr 115 Lys Asn SIle SIle Val 195 Asp Pr o Pr o Lys Al a 275 Ar g Ser Val Ser Pr o 355 Al a SIle Pr o Phe Tr p 435 Ser Gy Thr Gy Al a 165 Leu Phe Ar g SIle Ser 245 Met l Ie G n Phe Pr o 325 bet Ser Leu Leu Tyr 405 Pr o Val Leu Ser Tr p 135 Gy Pr o Cys Tr p Ser 215 Leu Tr p SIle Gy Ser 295 Al a Leu SI e Thr Al a 375 Thr Ser SIle Net Gy Al a 120 Thr Val Al a Gy Thr 200 Phe Gy Leu SIle Ar g 280 Lys Leu Ser SIle Al a 360 Val Ser Al a Thr Gy 440 Ser 105 Thr Al a Al a Al a Gy 185 Ar g Gy I e Lys Val 265 Lys Asp Al a Asn Val 345 Thr Gy Thr Al a Tyr 425 Phe Al a Val G u Leu Al a 170 Al a Lys G u Leu Thr 250 Tr p Ar g Leu Al a Tr p 330 Cys Al a SIle G u l Ie 410 Phe Leu Gy Ar g Pr o 155 Al a Tyr Asp Al a SIle 235 Hi s Val SIle Pr o Al a 315 Leu SIle Al a Asp Gy 395 Al a Val SIle Ser Ser 140 Pr o Ser Al a Lys bet 220 Pr o Gy Pr o Al a G n 300 Asn Asp bet Al a Pr o 380 Al a Asp Val Gy Val 125 Al a Asn Ser Val SIle 205 Lys Val Val SIle Asn 285 Phe SIle Ser Cys l Ie 365 Thr Ser Al a Pr o I e I e Leu Thr Ser 175 Tyr Al a Gy Leu G u 255 I le et Thr G u Asp 335 Val Al a Al a Pr o Pr o 415 I le Al a Pr o Val et 160 G n Ar g Thr Tr p Thr 240 Ser Thr Al a Val G u 320 Leu Val Pr o SIe Val 400 Thr Leu Leu Pro Val lie Val Pro Thr 445 Page 654 Gene Sequence 24-2-06.txt <210> 509 <211> 1203 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1180) <223> RXC00412 <400> 509 cttttgacga acaccacgtc gcgtacgctt cctcggggcg cttttgtccc ccgacttttg tacgaatcga ggacaccgt c ttaaactatt tgtcttccag t cc Ser ggc Gy aaa Lys ccc Pr o act Thr ttg Leu cgt Ar g ttc Phe gcc Al a gag G u 150 tcg Ser aat Asn ccg Pr o cgc Ar g gct Al a gag G u gt c Val ct c Leu ct g Leu tcg Ser 120 at g et gt c Val ggc Gy acg Thr cca Pr o gag G u acc Thr at c SIle ct c Leu ggc Gy agt Ser act Thr aag Lys ct g Leu aag Lys 170 ttg Leu gag G u tt c Phe acc Thr ggc Gy at c I e 75 acc Thr aat Asn gcg Al a gca Al a ggc Gy 155 cag G n ctt Leu gaa G u cgc Ar g gcg Al a at c SIle aat Asn gac Asp at c SIle gct Al a gct Al a 140 gac Asp cgc Ar g gcc Al a t ac Tyr ggc Gy ctt Leu 45 at c SIle ggc Gy at c SIle ggc Gy gga Gy 125 cgt Ar g aaa Lys gt c Val gac Asp tcc gcg Ser Al a 15 ata acc Ile Thr gat aat Asp Asn ggt tac G y Tyr ctt gac Leu Asp gtc gga Val G y 95 atg att let Ile 110 aat gt g Asn Val aaa gct Lys Al a ggc aaa G y Lys ggc att G y lle 175 gaa gcc G u Al a cag G n aaa Lys gt c Val tct Ser t cc Ser 80 at g tet ttc Phe gag G u cgc Ar g aac Asn 160 gcc Al a gtg tca Val Ser 1 caa ccc G n Pro gtc ttt Val Phe act ct c Thr Leu ggc gcc G y Al a ccc acg Pro Thr ccc gag Pro G u cag cag G n n tac ccg Tyr Pro 130 gtg caa Val G n 145 tac ccc Tyr Pro cgt gca Arg Ala cac acc gcg Hi s Thr Al a agc acc cag Ser Thr G n agc aac aat Ser Asn Asn acc gta gaa Thr Val G u ggc aag tcc G y Lys Ser agc ggt tcg Ser G y Ser tct aag ctg Ser Lys Leu 100 ttc aac ctg Phe Asn Leu 115 ctg gaa gtt Leu G u Val gaa at g ct c G u let Leu gag cag ctg G u G n Leu 165 ctg gcc acc Leu Al a Thr 180 acc tcc gct ttg gac cca Thr Ser Ala Leu Asp Pro Page 655 Gene Sequence 24-2-06.txt gaa acc acc cat gaa gtt ctg gag ctg c G u Thr Thr His G u Val Leu G u Leu L 200 205 ctg ggc atc acc atc gtt gtg atc acc c Leu Gy Ile Thr Ile Val Val Ile Thr H- 215 220 tcc atc gca gac aag gtt gct gtg atg g Ser Ile Ala Asp Lys Val Ala Val IVet C 230 235 tac ggc agc gtc tac gag gtg ttc tcc a Tyr y Ser Val Tyr G u Val Phe Ser A 250 2 caa aag ttc gtg gcc acc gcg ctg cgt a G n Lys Phe Val Ala Thr Ala Leu Arg A 265 270 tcg gaa gat ctg ctt agc cat gag gga c Ser G u Asp Leu Leu Ser His G u G y A 280 285 act gaa acg tcc ggc ttc ttt gca gca a Thr G u Thr Ser G y Phe Phe Al a Al a T 295 300 ggt gct ttt gtc aac atc gtt cac ggt g G y Ala Phe Val Asn IIe Val His G y C 310 315 caa tca ttt ggc aaa atg act gtt cga c G n Ser Phe y Lys IVet Thr Val Arg L 330 3 att gaa gag ttc tat caa acc ttg acc a lie G u G u Phe Tyr G n Thr Leu Thr L 345 350 atc acc cga tgaacgagat gatcctcgca gct I e Thr Ar g 360 <210> 510 <211> 360 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 510 Val Ser His Thr Ala Ser Thr Pro Thr P 1 5 G n Pro Ser Thr n G y Thr Arg Val C 25 Val Phe Ser Asn Asn Lys Ser Ala Lys T 40 Thr Leu Thr Val G u Pro Gy G u Val I 55 Sy Ala y Lys Ser Thr Leu Val Arg L 70 F cgc Ar g gaa G u t cc Ser 240 cca Pr o acc Thr ct g Leu gct Al a gt g Val 320 acc Thr acc Thr aag Lys at g bet 225 ggc Gy caa G n cca Pr o ttc Phe cgt Ar g 305 acc Thr ggc G y acg Thr gt a Val 210 gaa G u aaa Lys aca Thr gac Asp acc Thr 290 gct Al a acc Thr aac Asn acc Thr cgc Ar g gt g Val gt g Val gtt Val 260 gt g Val gat Asp gaa G u caa G n gct Al a 340 aag Lys gaa G u cgt Ar g gaa G u 245 gct Al a gaa G u ct g Leu caa G n cgc Ar g 325 gcg Al a gag G u 739 787 835 883 931 979 1027 1075 1123 1171 1203 'ro G u 10 u Phe hr Thr le y eu Il e 75 'age 65E O u Tyr Ser Ala G n Arg y lie Thr Lys Ala Leu Asp Asn Val lie Ile G y Tyr Ser Asn G y Leu Asp Ser Pro Thr Ser y Ser Leu Leu Gene Sequence 24-2-06.txt Leu Asn G y Thr Asp lie Val G y let Pr o G n Tyr Val 145 Tyr Ar g Ser Lys let 225 Gy G n Pr o Phe Ar g 305 Thr Gy Thr Ser Phe 115 Leu G u G u Leu Leu 195 Asn Val Val G n G n 275 I le Al a Leu Thr SIle 355 Leu Leu Val Leu Leu 165 Thr Pr o G u Ar g G u 245 Al a G u Leu G n Ar g 325 Al a G u Lys G n Lys 135 Phe Sy Pr o Thr Sy 215 I le Sy Lys G u G u 295 Al a Ser G u Thr Ar g 105 Ar g Asp Sy G n Leu 185 Hi s Thr Asp Val Val 265 Leu Ser Val Sy Phe 345 Asn Al a Al a Sy 155 G n Leu Val Val Val 235 G u Thr Ser Phe SIle 315 bet G n SIle Al a Al a 140 Asp Ar g Al a Leu Val 220 Al a Val Al a Hi s Phe 300 Val Thr Thr Sy Sy 125 Ar g Lys Val Asp G u 205 I le Val Phe Leu G u 285 Al a Hi s Val Leu SIe Val Al a Lys SIe 175 Al a Leu Hi s G u Asn 255 Asn Ar g Thr Sy Leu 335 Lys <210> 511 <211> 813 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(790) <223> RXC00526 <400> 511 ggtggagcag gcggcggctc cttttagtcc tgcggcccct tttgaccctg cagcccctgc Page 657 cgtttctgcc aagcaaaccg tgggc( Gene Sequence 24-2-06.txt caggt gatttagcct atg agc ctc atc gaa IVet Ser Leu Ile G u at g WMbt acc Thr tcg Ser at t SIle gt g Val aaa Lys gat Asp gct Al a at g et aag Lys 150 at t SIle at g Net at c SIle gt g Val cga aat Arg Asn gtg ttg Val Leu gtt gtg Val Val ggg ttg G y Leu gat gtg Asp Val tcg att Ser Ile gcg ttg Al a Leu aag cag Lys G n 120 ggt gag G y G u 135 cag cgc G n Ar g ctt gct Leu Al a gtg at g Val lVet gtg ttt Val Phe 200 gtc acc Val Thr 215 gt c Val ggt Gy acg Thr gat Asp gat Asp ttt Phe aat Asn aga Ar g ct c Leu gcc Al a gaa G u 170 at t SIle act Thr gtt Val acc Thr gat Asp ggc Gy cca Pr o agc Ser ttt Phe gaa G u cgt Ar g cat Hi s 140 gct Al a act Thr cac Hi s aac Asn ggg G0y 220 aac Asn cat Hi s ggt Gy gat Asp gat Asp aac Asn ccc Pr o 110 gtt Val ccc Pr o gcg Al a gcg Al a ct c Leu 190 gag Gu at c SIle gga Gy gac Asp t ca Ser acg Thr ttg Leu aat Asn at g tet tta Leu gag G u gcg Al a 160 gat Asp aag Lys gct Al a ggg Gy gaa G u ggc Gy at g let acc Thr agc Ser at t SIle gcg Al a gaa G u 130 tcg Ser gat Asp gca Al a cag G n gaa G u 210 gag G u ggt Gy gaa G u at g tet ttg Leu cac Hi s ggc Gy ggc Gy 115 at g bet ggt Gy cct Pr o acg Thr ggc Gy 195 tct Ser gt g Val gaa G u ttc Phe aac Asn gat Asp cgc Ar g cgg Ar g 100 att SIe gtc Val ggt Gy gag G u ggc Sy 180 aaa Lys gat Asp aaa Lys tca tgagccttgc agaatcaatt ctt Ser 230 <210> 512 <211> 230 <212> PRT Page 658 Gene Sequence 24-2-06.txt <213> Corynebacter i um gl ut am cum <400> 512 IVet Ser Leu Ile Gu IVet Arg Asn Ile 1 Ser Ar g Thr Tyr Al a Leu Tyr Leu Leu 145 Asn Ser G u Asp Ser 225 Gy G u bet Leu Hi s Gy Gy 115 Met Gy Pr o Thr Gy 195 Ser Val 5 Leu Val SIle Gy Al a I le Pr o Gy G n SIle 165 Ar g Thr Ar g Hi s Thr Ser SIle Val 70 Lys Asp Al a bet Lys 150 I le bet SIle Val Ser 230 Val Val Gy Asp Ser Al a Lys Gy 135 G n Leu Val Val Val 215 Hi s Gy Leu Leu Gy Lys 105 Ar g Ar g Val Asp Asp 185 I le bet Val 10 Gy Thr Asp Asp Phe 90 Asn Ar g Leu Al a G u 170 I le Thr Val Lys Val Ser Lys SIle 75 Val Val Ser Asn l Ie 155 Pr o Phe Hi s Asp Thr Asp Gy Pr o Ser Phe G u Ar g Hi s 140 Al a Thr Hi s Asn Gy 220 Tyr Phe Ser Thr Asp G n bet Al a 125 G u Ar g Gy G n Pr o 205 Ar g Asn Hi s Gy Asp Asp Asn Pr o 110 Val Pr o Al a Al a Leu 190 G u lie I e Val Lys Gy Al a Phe bet G u Asn Leu Leu 175 Asn Leu SIle Gy Asp Ser Thr Leu Asn bet Leu G u Al a 160 Asp Lys Al a Gy <210> 513 <211> 1185 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1162) <223> RXC01004 <400> 513 ccggacgctg gatcgcacga gtaacggtca tggaagatcg acgcatcgac aaagccgtt tcacccccat cacccatgaa gaagcaaagg agtacgaaaa gtg agt att tgg gca Val Ser Ile Trp Ala 1 act gtc ctt cta att atc gtc ctt ctt tcc gcc aac gcc ttc ttc gtg Thr Val Leu Leu lie Ile Val Leu Leu Ser Ala Asn Ala Phe Phe Val Page 659 c 115 163 Gene Sequence 24-2-06.txt gcc Al a ct g Leu gag G u tgt Cys at t SIle cca Pr o ct c Leu acc Thr acc Thr 150 cgc Ar g cca Pr o ctt Leu cag G n ct g Leu 230 gt c Val t cc Ser gat Asp gag Gu t cc Ser ct c Leu ct g Leu gt g Val t cc Ser ggt Gy 120 ggc Gy ccg Pr o ttt Phe cag G n gct Al a 200 t cc Ser ttc Phe gag G u ttg Leu gag G u gca Al a ggt Gy at c SIle ct g Leu ttc Phe gt c Val at g Net tgg Tr p at c SIle gt g Val 170 gca Al a gag G u aag Lys aaa Lys ggt Gy 250 t at Tyr gat Asp ct g Leu aaa Lys at g bet ggt Gy 75 acc Thr at c SIle gt g Val ctt Leu gag G u 155 gag G u t ca Ser cac Hi s gaa G u tct Ser 235 tt c Phe at c SIle ccc Pr o at t SIle aag Lys ttg Leu aaa Lys t cc Ser gca Al a cca Pr o gct Al a 140 ttc Phe caa G n at g Net gcc Al a ct g Leu 220 ggc Gy t cc Ser cac Hi s t cc Ser t cc Ser gga Gy 45 gcg Al a gt c Val tgg Tr p ct g Leu aag Lys 125 cca Pr o at g Net aaa Lys at t SI e cgc Ar g 205 gt g Val ccg Pr o cgc Ar g at c SIle gag G u tcg cgc cg Ser Arg Ar gct gaa aa Ala G u Ly ggc gct ca G y Al a G gca gaa cc Al a G u Pr 8 ggt gtt cc Sy Val Pr 95 gcg at c at Al a lle II 110 aac atc gc Asn Ile Al gt g ct c at Val Leu II aac t gg at Asn Trp II 16 aac gag ct Asn G u Le 175 t cc gag t c Ser G u Se 190 ct g t cc aa Leu Ser Ly att aag ga Ile Lys As acc ttg ca Thr Leu Hi 24 ttc cct gt Phe Pro Va 255 aag gat at Lys Asp II 270 acc att cc Thr Ile Pr g g 's ig n t 0 o 10 a o c e ;t a t e c e 0 g !u c *r ig 's it 'P c s -0 -O c l t e gac Asp gtt Val ttc Phe gcg Al a aat Asn acc Thr at t l Ie gcg Al a 145 gcc Al a gat Asp cgt Ar g gcg Al a gag G u 225 cag G n acc Thr ttg Leu cgc Ar g ct c Leu ggt Gy at c l Ie gat Asp tgg Tr p gct Al a 130 ttt Phe cgt Ar g t cc Ser t cc Ser ct g Leu 210 gac Asp ttg Leu ggc Gy cct Pr o gat Asp gca Al a acg Thr cac Hi s at c SIle 100 cac Hi s cct Pr o aag Lys acc Thr gt g Val 180 ggc Gy tct Ser cgc Ar g gaa G u gat Asp 260 ct g Leu t cc Ser acc Thr gtc Val ttc Phe cac Hi s att SIe gaa G u att SIe ctt Leu 165 gac Asp ct c Leu gag G u acg Thr gca Al a 245 gga Gy gct Al a :g cgt tct gca ctg cgc o Arg Ser Ala Leu Arg Page 660 280 cct ttg agc aat gtg Pro Leu Ser Asn Val 295 ttt atg cac tac cgc Phe Met His Tyr Arg 310 gag ctt ctc ggc gtg G u Leu Leu G y Val 330 ggc acc gtc aac gat Sy Thr Val Asn Asp 345 aactgtgttg gac <210> 514 <211> 354 <212> PRT gcc Al a 300 gcg Al a acg Thr act Thr Gene Sequence 24-2-06.txt 285 290 gac ggc ctc atg gat gac gtc ttg gat Asp y Leu IVet Asp Asp Val Leu Asp 305 cac atg gct cag gtt cgc ctc aaa ggt His et Ala G n Val Arg Leu Lys G y 320 325 ctg gag gat ctc atc gaa gaa tac gtg Leu G u Asp Leu Ile G u G u Tyr Val 335 340 cac gaa agc tcc gac gac tagaaatagt His G u Ser Ser Asp Asp 350 1027 1075 1123 1172 1185 <213> Corynebacter i um gl ut ai cum <400> 514 Val Ser Ile Trp Ala Thr 1 Asn Asp Val Phe Al a Asn Thr SIle Al a 145 Al a Asp Ar g Al a Phe Leu Tyr SIle Al a Leu Leu 115 Sy Val Leu Thr G u 195 Ar g 5 Val Ser Thr Val Phe Hi s SIle G u SIle Leu 165 Asp Leu G u Al a Leu G u Cys 70 I le Pr o Leu Thr Thr 150 Ar g Pr o Leu G n Val Al a Val Hi s Ser G u SIle Phe Leu 135 Ar g Al a G u Asp Ar g 215 Leu G u Ser 40 Leu Leu Val Ser Sy 120 Sy Pr o Phe G n Al a 200 Ser Leu Phe G n Ser SIle Pr o Phe 105 G u vet Leu Sy Leu 185 Su lie I e 10 Al a Gy I e Leu Phe 90 Val bet Tr p SIle Val 170 Al a G u SIle Leu Lys vet Sy Thr SIle Val Leu G u 155 G u Ser Hi s Val SIle Lys Leu Lys Ser Al a Pr o Al a 140 Phe G n Aet Al a Leu Ser Sy Al a Val Tr p Leu Lys 125 Pr o vet Lys SI e Ar g 205 Leu Ser Al a Sy Al a Sy Al a 110 Asn Val Asn Asn Ser 190 Leu Ser Ar g G u Al a G u Val l e I e Leu Tr p G u 175 G u Ser Lys G u Leu Val lie Lys Asp 220 Page 661 G u 225 G n Thr Leu Ar g Asp 305 Val SIle Asp Val G u Ar g Ar g 275 Al a Val Leu G u Thr Al a 245 Gy Al a Ar g Asp Gy 325 Val Al a Ar g Tyr Pr o Leu 295 Met Leu Thr Gene Sequence 24-2-06.txt Phe Gy Lys Ser y Pro Thr 235 G u Thr y Phe Ser Arg Phe 250 Leu G y Tyr li e His lie Lys 265 270 G u IVet Asp Pro Ser G u Thr 280 285 Ser Asn Val Asp Ala Asp y 300 His Tyr Arg Ser Ala His IVet 315 Leu y Val lie Thr Leu G u 330 Val Asn Asp Trp Thr His G u 345 350 Leu Pr o 255 Asp SIe Leu Al a Asp 335 Ser <210> 515 <211> 732 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(709) <223> RXC01017 <400> 515 gaaatttgag ggggcgctac ccttagaagg tgcgcaatga agtgtggatt gctagaaaac tttaagaaag aggaaataat acc Thr tgg Tr p cca Pr o t ac Tyr gct Al a at g bet tgg Tr p aag Lys agc Ser gag G u gcc Al a acc Thr gat Asp gt c Val gct Al a at g et gac Asp at c SIle acc Thr gaa G u gt c Val aag Lys cat Hi s 75 cac Hi s acc Thr caa G n ct a Leu gct Al a gct Al a aac Asn tgc Cys gt c Val aac Asn 45 gca Al a gac Asp gac Asp cca Pr o cgc Ar g gaa G u tgg Tr p aag Lys ggt Gy ttc tgc Phe Cys 15 gat at t Asp II e ggc cgt G y Arg gga cca G y Pro ctc ggc Leu G y 80 cgc gga Arg G y Page 662 caccacgata gt t cgcgcct atg gct caa aaa gta Net Ala G n Lys Val 1 tgg gtc acc tcc cgc Trp Val Thr Ser Arg gaa atc cag tgg gtt Su Ile n Trp Val gat ctc cca gag gat Asp Leu Pro G u Asp gca cgc gtt ttc gca Ala Arg Val Phe Ala gac ctg tac acc gca Asp Leu Tyr Thr Ala cca atc gaa ggt tcc Pro lie u y Ser Gene Sequence 24-2-06.txt 95 100 ttc aat gat gtc atc gca gag gca ctt gaa gag gtc ggc cta gac gct 451 Phe Asn Asp Val Ile Al a u Al a Leu G u u Val Gy Leu Asp Ala 105 110 115 gca ctt ggt gaa gtt gca gac acc acc gaa tgg gac gac gca ctt cgc 499 Ala Leu Gy G u Val Al a Asp Thr Thr G u Trp Asp Asp Ala Leu Arg 120 125 130 gca ttc cac cag acc gca atg gac gag gtc ggc aac gat gtc gga acc 547 Ala Phe Hi s n Thr Al a Net Asp G u Val G y Asn Asp Val G y Thr 135 140 145 cca gtg gtc aag ctc ggc gac acc gct ttc ttc ggc cca gtg ctc acc 595 Pro Val Val Lys Leu G y Asp Thr Ala Phe Phe G y Pro Val Leu Thr 150 155 160 165 cgc atc cca cgc ggc gag gaa gca gga gag atc ttc gac gct tcc ttc 643 Arg lie Pro Arg Gy Gu Gu Ala Gy u Ile Phe Asp Ala Ser Phe 170 175 180 aag ctc gca agc tat ccc cac ttc ttt gaa atc aag cgc agc cgc act 691 Lys Leu Ala Ser Tyr Pro His Phe Phe G u Ile Lys Arg Ser Arg Thr 185 190 195 gag aac cca cag ttc gac taattaacgc tgtctctgct tat 732 G u Asn Pro G n Phe Asp 200 <210> 516 <211> 203 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 516 IVet Ala G n Lys Val Thr Phe Trp Phe Asp Thr Thr Cys Pro Phe Cys 1 5 10 Trp Val Thr Ser Arg Trp lie Lys u Val G u G n Val Arg Asp Ile 25 G u Ile G n Trp Val Pro IVet Ser Leu Ala Val Leu Asn G u y Arg 40 Asp Leu Pro G u Asp Tyr Lys G u Arg IVet Lys Ala Ala Trp y Pro 55 Ala Arg Val Phe Ala Al a Val Ala Thr Asp His Ala Asp Lys Leu G y 70 75 Asp Leu Tyr Thr Ala Iet G y Thr Arg lie His Asn Asp G y Arg G y 90 Pro Ile G u Gy Ser Phe Asn Asp Val Ile Ala Gu Ala Leu G u u 100 105 110 Val y Leu Asp Ala Al a Leu Gy G u Val Ala Asp Thr Thr G u Trp 115 120 125 Asp Asp Ala Leu Arg Al a Phe His G n Thr Ala IVet Asp G u Val G y 130 135 140 Asn Asp Val y Thr Pro Val Val Lys Leu y Asp Thr Ala Phe Phe 145 150 155 160 Sy Pro Val Leu Thr Arg Ile Pro Arg Gy G u G u Ala Gy G u lie Page 663 Gene Sequence 24-2-06.txt 165 170 175 Phe Asp Ala Ser Phe Lys Leu Ala Ser Tyr Pro His Phe Phe u Ile 180 185 190 Lys Arg Ser Arg Thr u Asn Pro G n Phe Asp 195 200 <210> 517 <211> 622 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(622) <223> RXC01021 <400> 517 cgagaggctt ttttggctct aagcctttta gtcgtgcgaa gtgccaccga gatcgat t gg tcgctgtaag gtatctgat t cgaaatctta agcagcctcg atg tcc agt tcc gaa 11! IVet Ser Ser Ser G u agc Ser cgc Ar g t cc Ser gt c Val ttt Phe aca Thr gca Al a gga Gy gca Al a t cc Ser 150 ggc Gy cgt Ar g gct Al a caa G n cag G n gtt Val gcg Al a at t l Ie tct Ser 120 gct Al a gct Al a gtt Val gaa G u t ca Ser gac Asp tct Ser gat Asp gat Asp gag G u gt a Val ggg Gy t cc Ser act Thr ggc Gy acg Thr gct Al a gaa G u gca Al a 75 gaa G u gct Al a gt c Val gtt Val ct a Leu 155 gt g Val tcg Ser ggg Gy gcc Al a cgt Ar g gac Asp gcg Al a ttg Leu ctt Leu at t SIle 140 gtt Val ttg Leu cag cca G n Pro gct cct Ala Pro gga gtt G y Val 45 cgt gca Arg Ala act gga Thr G y ctg aaa Leu Lys gct gtt Al a Val 110 agt cag Ser G n 125 gat ggc Asp y gat cgc Asp Arg cgt tct Arg Ser gca Al a 15 gcg Al a ctt Leu gag G u t ca Ser ct g Leu 95 ggt Gy gt c Val cac Hi s gca Al a ccg Pr o gca Al a gag G u at c lile aac Asn 80 ggg Gy tct Ser ctt Leu aca Thr at t l Ie 160 1 tct Ser gct Al a tgg Tr p aat Asn at g Net gag G u gt g Val cgc Ar g at c SIle 145 aca Thr gt a Val t cc Ser gcc Al a gca Al a acc Thr ttg Leu cgt Ar g gct Al a 130 caa G n gat Asp cag G n aag Lys agg Ar g ct g Leu t ac Tyr ggt Sy gga Sy 115 at t l Ie gaa G u cct Pr o cct Pr o gaa G u acc Thr aat Asn acc Thr gat Asp 100 gcc Al a gct Al a gcg Al a gt g Val gaa G u gct Al a gct Al a gtc Val at g bet gtc Val cga Ar g cag G n ct a Leu gag G u 165 Page 664 Gene Sequence 24-2-06.txt 170 <210> 518 <211> 174 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 518 Met Ser Ser Ser u Ser Ser Arg Ser G u Gy Ser G n Pro Ala Pro 1 5 10 Ser Val G n Pro G u Arg Arg Ala Asp Ser Thr Gy Ala Pro Ala Ala 25 Al a Ser Lys G u Al a Ser n G n Met Asp Al a Ala y Val Leu G u 40 Trp Ala Arg Thr Ala Val G u G n Leu Ser G u Arg Arg Ala G u lie 55 Asn Ala Leu Asn Val Phe Pro Val Pro Asp Ala Asp Thr G y Ser Asn 70 75 IVet Thr Tyr Thr IVet Thr Al a Al a Leu Asp G u Al a Leu Lys Leu G y 90 G u Leu y Asp Val Al a Arg Ile Thr G u Ala Leu Ala Val y Ser 100 105 110 Val Arg y Ala Arg G y Asn Ser y Val Val Leu Ser G n Val Leu 115 120 125 Arg Ala IIe Al a n Ala Ala Ala Asp G y Val Ile Asp G y His Thr 130 135 140 lie n u Ala Leu Ser lie Ala Arg Ser Leu Val Asp Arg Ala lie 145 150 155 160 Thr Asp Pro Val G u G y Thr Val Val Thr Val Leu Arg Ser 165 170 <210> 519 <211> 1047 <212> DNA <213> Cor ynebact er i um gl ut ai cum <220> <221> CDS <222> (101)..(1024) <223> RXC01212 <400> 519 tttagaagcc acat gacat a t gt cat gaaa attatgtgca aagt gcagt a atactcctga catatggctc taccagcgcc aatgcgaagt aggaagaatt atg cct atg aca acg 115 IVet Pro IVet Thr Thr 1 aca cca gca atc gac gta aca gac ctc gtg aga acc tac ggc gac tac 163 Thr Pro Ala lie Asp Val Thr Asp Leu Val Arg Thr Tyr y Asp Tyr 15 acc gca gtc aag ggc ctg aat ttc cat gta cag cgc ggt gaa gta ttt 211 Thr Ala Val Lys G y Leu Asn Phe His Val G n Arg G y G u Val Phe 30 Page 665 ene Sequence 24- 2- 06. txt ggt ct g ct C ggc acc aac ggg gcc ggc aaa acc G3 y Leu Leu G3 y Thr Asn G3 y A a G3 y Lys Thr t cc acc t tg gaa gt c Ser Thr Leu a u Val at c I Ie ct t Le u at g vb t at g vb t gt g ValI gcg A a ct t Le u 1 50 g ac As p c ag a31n g aa a31u gt g ValI agt Ser 230 gct A a ct g Le u at c I Ie gga (31y Pr o c aa a31n at g vb t gcc A a 120 t cc Ser g ac As p g aa a31u a31y ct c Le u 200 ggc a31y gt g ValI at c I Ie c ag a31n ct g Le u 280 t cc Ser gcc A a ggc a31y 90 c ac Hi s gt c ValI ggc (31y t ca Ser ag g Ar g 1 70 ac c Th r g ac As p t tg Le u cgt Ar g cgc Ar g 250 ac c Th r ggc a31y gca A a gac As p 75 ggc a31y a31y gac As p gaa (31u at t I Ie 155 cgc Ar g at g Ar g g at As p ggc a31y 235 gac As p t t a Le u t t c Ph e CCC Pr o 60 cgc Ar g ct g Le u ac c Th r ct c Le u c aa G n 140 tt g Le u c ac Hi s at g vb t at t I Ie g aa a3u 220 ggg a3y aac As n g aa a3u gct A a ag c Ser gcg A a cca Pr o t gc cys ct a Le u 125 cga Ar g tt c Ph e ac c Th r ct g Le u gcc A a 205 ct g Le u c ag a31n aac As n at a I Ie gca A a 285 t cc Ser at c I Ie t ca Ser ac g Th r 110 c ac Hi s cgc Ar g ct c Le u t gg Tr p ac c Th r 1 90 at c I Ie gt g ValI gt g ValI c ac Hi s ct t Le u 270 aaa Ly s ac c Th r cgc Ar g 80 ct c Le u ccg Pr o g aa a31u g at As p g aa a31u 1 60 ct c Le u c ac Hi s aac As n cgc Ar g tt g Le u 240 cgc Ar g t gg Tr p gcc A a t cg Ser gt g ValI CCC Pr a ac c Th r cgc Ar g aac As n tt g Le u 145 CCC Pr a ct g Le u t ac Ty r gcc A a gag a31u 225 ccg Pr a at c I Ie gct A a ac c Th r ct g Le u 305 cgc Ar g gag a31u gt c ValI gcc A a gt c ValI 130 gcc A a ac c Th r ct g Le u ct g Le u ggt a31y 210 aag Ly s gt c ValI gcc A a gca A a tt g Le u 290 c aa a31n at c I Ie ct c Le u gcc A a at t I Ie 115 aag Ly s t gc cys ac c Th r g ac As p gag a31u 1 gag a31u t cg Ser tt g Le u ac c Th r gag a31u 275 g aa a31u ac c Th r t cc Ser ggc a31y g aa a31u 1 00 aaa Ly s gt c ValI gca A a ggc a31y ct g Le u 1 g aa a31u at c I Ie at c I Ie agt Ser ac c Th r 260 ac c Th r t cc Ser gcc A a ggg (31y at c lIe ac c Th r g at As p ggc a31y ct g Le u ct c Le u 1 aaa Ly s gcc A a gca A a at c I Ie ggg a31y 245 ac c Th r ggg a31y gt a ValI ttc atg gac atc gcc tca ctc gag aac Phe l'bt Asp I Ie Ala Ser Leu a31u Asn 1 024 Page 666 Gene Sequence 24- 2- 06. txt tagaatcttt aaggagacca caa 1047 <210> 520 <211> 308 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 520 IVet Pro IVet Thr Thr Thr Pro Ala lie Asp Val Thr Asp Leu Val Arg 1 5 10 Thr Tyr G y Asp Tyr Thr Ala Val Lys G y Leu Asn Phe His Val O n 25 Arg Gy G u Val Phe G y Leu Leu y Thr Asn Gy Ala Gy Lys Thr 40 Ser Thr Leu G u Val I le u y Leu Ser Ala Pro Ser Ser y Thr 55 Val Arg Ile Ser y Leu Asp Pro Val Ala Asp Arg Ala lie Leu Arg 70 75 Pro G u Leu y Ile IVet Leu G n Ser G y y Leu Pro Ser G n Leu 90 Thr Val Al a G u Thr IVet Asp IVet Trp Hi s G y Thr Cys Thr Tyr Pro 100 105 110 Arg Ala lie Lys Asp Val Leu Ala Asp Val Asp Leu Leu His Arg G u 115 120 125 Asn Val Lys Val Gy Al a Leu Ser G y y G u n Arg Arg Leu Asp 130 135 140 Leu Ala Cys Ala Leu Leu y Asp Pro Ser Ile Leu Phe Leu Asp G u 145 150 155 160 Pro Thr Thr G y Leu Asp Pro G u Ser Arg Arg His Thr Trp G n Leu 165 170 175 Leu Leu Asp Leu Lys G n Arg G y Val Thr IVet IVet Leu Thr Thr His 180 185 190 Tyr Leu Gu u Ala u Phe Leu Cys Asp Arg lie Ala lie IVet Asn 195 200 205 Al a y u Ile Ala Val G u y Thr Leu Asp u Leu Val Al a Arg 210 215 220 G u Lys Ser Ile Ile Ser Phe Val Leu Arg y y G n Val G u Leu 225 230 235 240 Pro Val Leu Ser G y Ala G u IIe IIe Arg Asp Asn Asn His Val Arg 245 250 255 lie Ala Thr Thr Thr Leu n G n His Thr Leu G u Ile Leu Thr Trp 260 265 270 Al a Al a G u Thr G y Ile Al a Leu G u G y Phe Al a Al a Lys Pro Al a 275 280 285 Thr Leu u Ser Val Phe IVet Asp Ile Ala Ser Leu G u Asn Thr Ser 290 295 300 Leu G n Thr Ala 305 Page 667 Gene Sequence 24-2-06.txt <210> 521 <211> 1131 <212> DNA <213> Corynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(1108) <223> RXC01306 <400> 521 tgttttagag gtagagcgaa acgtgcctgt aaaactggcg cccagacaat caccccgctc cgaaggagaa tcgctaagcc ctcgctgtgc tcgaagacca atg act gaa tgg tat 11! IVet Thr G u Trp Tyr gt c Val gt c Val gag G u aat Asn gt g Val ttg Leu gt c Val gt c Val acg Thr ttt Phe 150 cgc Ar g gac Asp tta Leu at t SIle gt g Val ctt Leu ttc Phe ccg Pr o gcg Al a ggc Gy 120 ctt Leu cca Pr o gtt Val gat Asp gcc Al a ttc Phe acc Thr ct c Leu ttg Leu ttc Phe at t l Ie at g Net act Thr ct g Leu gaa G u 170 ct c Leu act Thr gct Al a tcg Ser at g bet ggt G y 75 gaa G u tt g Leu gct Al a at c SIle cag G n 155 act Thr cat Hi s at t SIle ttg Leu cgg Ar g ct c Leu gct Al a tgg Tr p tcg Ser ccg Pr o gcg Al a 140 tgg Tr p ccc Pr o gcc Al a ct a Leu ctt Leu tct Ser 45 gcg Al a at c l Ie gcg Al a ct g Leu aaa Lys 125 at t l Ie at c SIle gtt Val ct c Leu ct c Leu gca Al a t cc Ser ggc G y acg Thr cgt Ar g ttt Phe 110 t cc Ser ccc Pr o aac Asn gat Asp at t SIle 190 at c I e 15 gct Al a cgc Ar g gcg Al a aag Lys at a SIle 95 at c SIle tgg Tr p gca Al a aaa Lys cga Ar g 175 gag Gu gcg Al a agg Ar g gct Al a cag G n ccg Pr o 80 ccg Pr o gt a Val gca Al a cgg Ar g at g Net 160 gct Al a cat Hi s 1 ct g Leu cgg Ar g gcg Al a ttg Leu tgg Tr p ct g Leu acg Thr at c l Ie ggc Gy 145 gcg Al a gca Al a t cc Ser tct Ser aac Asn ttg Leu gga G y gtt Val gtt Val ttt Phe gcg Al a 130 ttc Phe aac Asn gct Al a cga Ar g gcg Al a cgg Ar g cga Ar g at c SIle cat Hi s at g bet ct g Leu 115 cat Hi s at t SIle gat Asp ggt Gy gaa 15u 195 ttt Phe tta Leu agc Ser acc Thr t at Tyr gca Al a 100 cac Hi s ccg Pr o aac Asn ttg Leu ggc Gy 180 act Thr ttc Phe gag G u ct c Leu at g let gct Al a gat Asp ttg Leu gag G u ctg Leu gtc Val 165 t at Tyr ggc Gy gct ctg gat cag caa tcc gcc gcc caa atc agc gga att atc aag ctg Page 668 Ala Leu Asp 200 Sn G n Ser Al a Gene Sequence 24- 2- 06. txt Ala n Ile Ser G y Ile I le Lys Leu gat aaa atc acg gtc ggt Asp Lys Ile Thr Val G y 215 agc gcc agc gcc acg gtt Ser Ala Ser Ala Thr Val 230 235 ggc agc ttg cgt gtg ctt Sy Ser Leu Arg Val Leu 250 gtc att cat gtg cga gac Val Ile His Val Arg Asp 265 tcg aag tgg tct cgc cca Ser Lys Trp Ser Arg Pro 280 cac caa gcg ctg gaa tac His G n Ala Leu G u Tyr 295 gtg ctt tcc gct gat ggg Val Leu Ser Ala Asp G y 310 315 cac atc ttg aaa tac ctg His lie Leu Lys Tyr Leu 330 gaa <210> 522 <211> 336 <212> PRT <213> Corynebacter i um gl <400> 522 Met Thr G u Trp Tyr Val 1 5 Leu Ser Ala Phe Phe Val Arg Asn Arg Leu G u G u Ala Leu Arg Ser Leu Asn Leu G y i e Thr IVet Val 70 Trp Val His Tyr Ala Leu Leu Val Net Ala Asp Val 100 Thr Phe Leu His Leu Val 115 caa G n 220 gct Al a at c SIle acc Thr at c SIle at g Net 300 aaa Lys ct g Leu gt g Val gcc Al a ggt G y 270 acc Thr gag G u gt g Val gca Al a gcc Al a 240 t cc Ser tcg Ser gct Al a aac Asn ggt G y 320 tct Ser 225 gca Al a cac Hi s cca Pr o gag G u gag G u 305 gt a Val cca Pr o gct Al a ctt Leu gac Asp acc Thr 290 cag G n at a SIle acg Thr cgc Ar g cca Pr o 260 aag Lys acg Thr agt Ser tgg Tr p cac Hi s agt Ser 245 cac Hi s gct Al a tta Leu gcg Al a gat Asp 325 787 835 883 931 979 1027 1075 tgg cct gca Tr p Pro Al a tcg gtg Ser Val 335 tagctaattt gaggtgcgct 1128 1131 ut am cum Val Leu Pro Ilie lie u 25 Thr Val G u 40 G u Leu Thr Thr Phe Al a IVet Pro Leu I le Al a Phe 105 lie y G u 120 Al a Thr I 10 Phe Al a I Thr Ser Leu et I Leu G y Phe G u 90 Ile Leu Net Al a I Page 669 Leu Leu Ser Al a SIle Al a Leu Lys 125 Leu Al a Ser Sy Thr Ar g Phe 110 Ser I e Al a Ar g Al a Lys SIe I e Tr p Ile Ala His Pro u Thr Ala 130 135 Gene Sequence 24- 2- 06. t xt Leu Arg Thr Ile Ala lie Pro Al a Ar g Gy 145 Al a Al a Ser Gy Ser 225 Al a Hi s Pr o G u G u 305 Val SIle Asp Gy G u 195 I le Phe G n Phe G u 275 Asp SIle Thr Leu Val 165 Tyr Gy Leu Hi s Ser 245 Hi s Al a Leu Al a Asp 325 Ar g Lys Thr Leu Lys 215 Al a Ser SIle Lys G n 295 Leu SIle Pr o Val Asp Asp 200 I le Ser Leu Hi s Tr p 280 Al a Ser Leu Leu Gy Thr 185 G n Thr Al a Ar g Val 265 Ser Leu Al a Lys G n 155 Thr Hi s Ser Gy Val 235 Leu Asp Pr o Tyr Gy 315 Leu 140 Tr p Pr o Al a Al a G n 220 Al a SIle Thr SIle bet 300 Lys Tr p I le Val Leu Al a 205 Thr G u Asp Leu Leu 285 Ar g Thr Pr o Asn Asp SIle 190 G n Leu Val Al a Sy 270 Thr G u Val Al a Lys Ar g 175 Su I e Thr G n Pr o 255 Al a Val Hi s Leu Ser 335 <210> 523 <211> 513 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(490) <223> RXC01366 <400> 523 atgcatgaaa acaaat t cta tgtgtgt t ga gctgccaaaa ggggt t ggcg cgccgat gat gactgtccaa acctaaacca aaggtctaaa ctttggcttc gtg agt cag ttt cgt Val Ser G n Phe Arg 1 cgt tgt tcc cgc cct ggt tgt ggc aag cct gcc gtc gca acc ctc acc Arg Cys Ser Arg Pro G y Cys y Lys Pro Ala Val Ala Thr Leu Thr 15 tac gca tat tcg gat tcc act gcg gtg gtt ggt cct ttg gcg cct gca Tyr Ala Tyr Ser Asp Ser Thr Ala Val Val y Pro Leu Ala Pro Ala 30 Page 670 Gene Seq gca gag ccc cat agt tgg gat ctg tgt Ala G u Pro His Ser Trp Asp Leu Cys 45 act gcg ccc ctt ggt tgg gag atg ctg Thr Al a Pro Leu G y Trp G u Met Leu 60 gat gac gat gag gat ctg acg gct ctt Asp Asp Asp G u Asp Leu Thr Ala Leu 75 gga cgc act gtg agt ggt ctg gtt cct G y Arg Thr Val Ser G y Leu Val Pro cat ccg gtg aac cgg agt gcg cgg atc His Pro Val Asn Arg Ser Ala Arg lie 105 110 agg ggt cat ctc tat gtt gtg cct gat Arg G y His Leu Tyr Val Val Pro Asp 120 125 tattcggatt gga <210> 524 <211> 130 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 524 Val Ser n Phe Arg Arg Cys Ser Arg 1 5 Val Ala Thr Leu Thr Tyr Ala Tyr Ser Pro Leu Ala Pro Ala Al a G u Pro His 40 His Ala G u Arg lie Thr Ala Pro Leu 55 Asn Asp lie Lys Val Asp Asp Asp G u 70 Ala Val Arg G u Ala G y Arg Thr Val G u Val G y G y Asn His Pro Val Asn 100 105 G n Lys Val His Arg Arg G y His Leu 115 120 G u Ser 130 <210> 525 <211> 614 <212> DNA <213> Cor ynebact er i um gl ut ari cum <220> <221> CDS luence 24-2-06. t xt gag cat cat gcc gag cgt att G u His His Ala G u Arg lie cgg gtg aac gac atc aaa gtc Arg Val Asn Asp IIe Lys Val gct cag gct gtt cgt gag gct Ala G n Ala Val Arg G u Ala 80 gaa gac gaa gtg ggc ggc aac Su Asp u Val G y y Asn 95 100 gcg gaa cag aag gtt cac cgc Ala G u G n Lys Val His Arg 115 cag gac gaa tca taaggtttgc G n Asp u Ser 130 Gy Ser Tr p Tr p Leu Gy Ser Val Cys Thr Asp G u Thr Leu Al a Val Gy Al a Leu bet Al a Val Ar g Pr o 125 Pr o Val G u Ar g Al a G u Al a G n Page 671 Gene Sequence 24-2-06.txt <222> <223> RXC01372 <400> 525 cag G n 1 cct Pr o gca Al a at t SIle aat Asn cat Hi s cca Pr o gag G u gac Asp ccg Pr o 145 gcc Al a gca Al a gaa Gu acc ttc gtc Thr Phe Val 5 cgc atc gt g Arg li e Val ccc agc att Pro Ser Ile gag gaa gt g G u G u Val ggc cga aaa G y Arg Lys ggc cga act G y Ar g Thr tgg acc cga Tr p Thr Ar g 100 at g gaa t ca Met G u Ser 115 ata ttc cat Ile Phe Hi s agg gct gtt Ar g Al a Val gcg caa agc Al a G n Ser 165 cgt ttg ctg Arg Leu Leu 180 aag gac ttg Lys Asp Leu 195 ctt Leu gcg Al a at t SIle gaa G u ct g Leu 70 gat Asp cga Ar g tt g Leu gac Asp gt c Val 150 tgc Cys gt g Val ccc Pr o tcg Ser t cc Ser tcg Ser cgc Ar g gt g Val ggc Sy ccg Pr o cca Pr o 135 tgg Tr p gac Asp cgc Ar g acc Thr at a SIle gac Asp 40 ct a Leu gca Al a gga Gy ct g Leu gaa G u 120 ttc Phe gcc Al a gga Sy gga G y ttg Leu agc Ser 25 cgc Ar g tcg Ser t ac Tyr ct c Leu gt a Val 105 ct c Leu at a SIle gaa G u cct Pr o cag G n ccc Pr o 10 act Thr ttg Leu ccg Pr o gt a Val gcg Al a 90 t ca Ser cgc Ar g gat Asp gag G u agc Ser 170 t ca Ser acg Thr ctt Leu gaa G u gaa G u gat Asp 75 at t l Ie gcc Al a gga Sy gac Asp gaa G u 155 aaa Lys gcc Al a gcc Al a tta Leu cac Hi s cgt Ar g cac Hi s gcc Al a gt g Val ccc Pr o gaa G u 140 ccc Pr o ggg Sy acg Thr gca Al a gat Asp ct c Leu gac Asp gca Al a aac Asn ct g Leu gag G u 125 ccc Pr o gga Sy gcg Al a acc Thr ggc Gy ctt Leu gcc Al a gaa G u cgg Ar g gtt Val gat Asp 110 cca Pr o ggg Sy at c SIle ct g Leu t at Tyr 190 ttg Leu tta Leu gac Asp ct a Leu at c SIle at c I e ttt Phe at t SIle gt g Val ccc Pr o aca Thr 175 t cc Ser tcg Ser gaa G u tgc Cys gtc Val gtg Val gcc Al a ccc Pr o acc Thr gta Val gat Asp 160 caa G n att SIe taaatggagc tattggaagg ctc <210> 526 <211> 197 <212> PRT <213> Corynebacteri um gl utami cum <400> 526 G n Asp Thr Phe Val Leu Pro Thr Leu Pro Thr Ala Ala y Leu Ser 1 5 10 Pro Ala Arg Ile Val Ala Ser lie Ser Thr Leu Leu Asp Leu Leu G u 25 Page 672 Al a I Ie As n Hi s Pr o G31u As p Pr o 145 Al a Al a (31u Pr o G31u G31y G31y Tr p vb t 115 li1e Ar g Al a Ar g Ly s 1 I Ie ValI Ly s Thr Ar g Ser Hi s ValI Ser 1 65 Le u Le u Ser Ser Ar g ValI G31y Pr o Pr o 135 Tr p As p Ar g ene Sequence 24- 2- 06. txt Asp Arg Leu Gu His Leu Ala 40 Leu Se r Pro G31u Ar g Asp G31u Al a Tyr Val Asp His Ala Arg (3 y Leu Al a I I e Al a As n Val 90 Leu Val Ser Al a Val Leu Asp 105 110 (31u Leu Ar g G31y Pr o G31u Pr o 120 125 Phe Ilie Asp Asp G31u Pro G31y 140 Ala G31u 31u G31u Pr o 31y Ilie 1 G y Pr o Ser Lys G y Al a Leu 1 70 (3 y (3 n Ser Al a Thr Thr Tyr 185 190 As p Le u I Ie I Ie Ph e I Ie ValI Pr o Thr 1 Ser <210O> 527 <21 1> 669 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 ODS <222> (646) <223> RXCO1659 <400> 527 ttgatgcgcg ccgcggtgag cagcctgccg atccagagcg t cgagat t ga t accgt ct ct ggat t ccgcc t gct cagt ac c ag (31n g aa G31u ac c Thr tt g Le u tt t Ph e gt g ValI aag Ly s ct g Le u g ac As p gct Al a t cc Ser ac g Thr cgc Ar g t gg Tr p cct Pr o gcg Al a c ac Hi s g aa G31u g at As p t tc Ph e gt c ggt Val G31y 15 t ct gat Ser As p t gg gaa Tr p G31u acg tt g Thr Leu Page 67,: t gaagaat ct gat gat t t ag gtg gcg ggg gtt gcg Val Al a G31y Val Al a 1 t tg gat t cc at g cca Leu Asp Ser I'b t Pr o t gg gt g t tg gcg gaa Tr p Val Leu Al a G31u gag at t t tg cgg ccg G31u Ilie Leu Ar g Pr o cca cct cgt t cc acg Pr o Pr o Ar g Ser Thr cgt Ar g ccg Pr o gat Asp ccg Pr o ttg Leu ccg Pr o 150 t cc Ser tgg Tr p Gene Sequence 24-2-06.txt gcg caa acc ttg gat ttg aag cat ttg gaa cca agc cgt Ala G n Thr Leu Asp Leu Lys His Leu G u Pro Ser Arg 75 gag cag cca gaa aag cca gcg ttt act ccc aat gct tcg G u G n Pro G u Lys Pro Ala Phe Thr Pro Asn Ala Ser 95 ttg tct cag ccg ttg gtg atc cgc ccc gag gag ccg ttg Leu Ser G n Pro Leu Val Ile Arg Pro G u G u Pro Leu 105 110 115 gtt cgc ggt gtg cag gaa agc cgc gga gtg gtc gag cca Val Arg y Val G n u Ser Arg y Val Val G u Pro 120 125 130 ggt gcg gat gat gtg gag tcg att gcg gag ggc gat cca Sy Ala Asp Asp Val u Ser Ile Ala u y Asp Pro 135 140 145 agc gat ctt tat ggc acg cgt gtg ctg cgt gat ctc aat Ser Asp Leu Tyr G y Thr Arg Val Leu Arg Asp Leu Asn 155 160 agt att ttc caa gat tcc acc gac gcg gat gag cca cca Ser Ile Phe G n Asp Ser Thr Asp Ala Asp G u Pro Pro 170 175 tagaaaactg gtgtttttcg gcc ctg Leu gaa G u 100 cag G n cgg Ar g gag G u ggt G y aaa Lys 180 <210> 528 <211> 182 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 528 Val Ala G y Val Ala G n Arg Phe Val 1 Leu Tr p G u Pr o Pr o Asn G u Val Gy 145 Ser Leu Leu Ar g Ar g Ser Leu 115 Pr o Pr o 5 Pr o G u Pr o Thr Lys G u Met Ser Ar g Al a SIle Al a Al a G u Leu Val Sy 135 Ser Val Lys 40 Leu G n G n Ser Ar g 120 Al a Asp Thr Sy Leu Thr Pr o G n 105 Sy Asp Leu Asp u Arg 10 Al a Val Tr p Ser Thr Pro Thr Asp Al a Leu Asp Leu G u Lys Pro 90 Pro Leu Val Val Gn u Asp Val G u 140 Tyr G y Thr 155 Page 674 SIle et Ser Ser Lys Al a SIle Ser 125 Ser Ar g Val Ser Tr p Thr Leu Thr Pr o Sy Al a Leu Gene Sequence 24-2-06.txt Asp Leu Asn Gy G n Ser Ser Il e Phe G n Asp Ser Thr Asp Ala Asp 165 170 175 Su Pro Pro Lys Lys Trp 180 <210> 529 <211> 981 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(958) <223> RXC01663 <400> 529 tattttgctg gttggtcaca gtggagcgct aaccccgaga at cct at cga ggcct aaaat cgtggcttga gtacgcactg ccagtaaggt gt gt gat gt g atg gaa ata agt gtc lMt Gu le Ser Val ttg Leu gcg Al a t ca Ser ttt Phe ct g Leu ccg Pr o gct Al a ccg Pr o gag G u ttg Leu 150 gcg Al a at c SIle tcg Ser gca Al a cca Pr o gtt Val cag G n gtt Val gag G u 120 gct Al a tct Ser cac Hi s gca Al a aaa Lys gcg Al a ct g Leu cct Pr o gag G u aat Asn gt g Val gac Asp cct Pr o ttc Phe 170 ct g Leu cag G n ccc Pr o gac Asp aga Ar g 75 gac Asp gcc Al a cag G n gag G u ggt G y 155 gat Asp at c SIle gca Al a gt g Val cct Pr o ttt Phe at t SIle ttg Leu tct Ser gag G u 140 tcg Ser ttc Phe ttg Leu gct Al a ct g Leu 45 gaa G u gcg Al a t at Tyr gct Al a gag G u 125 caa G n cag G n at c SI e gt g Val aaa Lys gtt Val cca Pr o cca Pr o ttt Phe gag G u 110 caa G n cgg Ar g cgc Ar g aag Lys gca Al a 15 aag Lys gaa G u gaa G u gaa G u gat Asp 95 ctt Leu cct Pr o ggc G y cgg Ar g gaa G u 175 ggc G y gct Al a gaa G u gcg Al a cca Pr o gat Asp act Thr caa G n gt c Val gag G u 160 gat Asp gt a Val t ca Ser ccg Pr o cca Pr o caa G n cct Pr o gt a Val cct Pr o 130 aag Lys cga Ar g ttt Phe ct g Leu cct Pr o gac Asp gaa gat Asp gaa G u gaa G u 115 gcc Al a cat Hi s aac Asn ttg Leu tgg Tr p gtg Val cct Pr o cca Pr o ctt Leu ct c Leu 100 gac Asp acg Thr tcg Ser tgg Tr p acc Thr 180 Page 675 gaa tgg tca agg ggt gcg gca G u Trp Ser Arg y Al a Al a 185 gtc agt ggc atg gct gaa gga Val Ser y Met Ala u y 200 ggc gtg ccc gtg atg gcg atg Sy Val Pro Val bet Ala 'bet 215 220 att gat gcg cgc cgc ggt gag lle Asp Ala Arg Arg Gy u 230 235 tct gat gat tta gtc gag att Ser Asp Asp Leu Val G u lie 250 agt acg tgg cgg ggg ttg cgc Ser Thr Trp Arg Gy Leu Arg 265 tcg gtt tgg att cca tgc cag Ser Val Trp Ile Pro Cys n 280 t ct <210> 530 <211> 286 <212> PRT <213> Corynebacter i um gl utar <400> 530 IVet Gu Ile Ser Val Leu Ile 1 5 lie Val Leu Trp Arg Ala Asp Su Ser Pro Val y Ser Val Su Pro Asp Pro u Phe u 55 Sn Pro u Pro u Leu u 70 Val G n Asp Leu G u Pro Asp Ser Pro G u Leu Asp Al a Asp 100 Su Val u Asp Tyr Pro u 115 Ala Pro Ala Thr Ala G u Val 130 135 Asp Lys His Ser Phe Leu Ser 145 150 Gene Sequence 24-2-06.txt t cg act ggt gcc gtt gca cgt gat Ser Thr Gy Ala Val Ala Arg Asp 190 195 tat gaa acg cat ctg gtg gat ttg Tyr G u Thr His Leu Val Asp Leu 205 210 cgc cgt gga att acc tct gac gtg Arg Arg Gy lie Thr Ser Asp Val 225 cag cct gcc gat cca gag cgt gaa Sn Pro Ala Asp Pro G u Arg u 240 gat acc gtc tct gga ttc cgc ctg Asp Thr Val Ser y Phe Arg Leu 255 260 agc gtt ttg tcg acg agc gca ttc Ser Val Leu Ser Thr Ser Ala Phe 270 275 aag ctg tgactgctgt gtggatggaa Lys Leu 285 gtg Val gcg Al a gtc Val gaa G u 245 ct c Leu acg Thr lle Ala Ala Leu Ile Leu Val Ala y Ser Pr o G u Al a Al a G u 105 Pr o Al a Leu 10 Lys G n Al a Al a Pro Val Leu Asp Pro Pro Arg Phe 75 G u Asp Ile 90 Asn Ala Leu Val G n Ser Asp G u u 140 Pro G y Ser 155 Page 676 Al a Leu G u Al a Tyr Al a G u 125 G n G n Lys G u G u G u Asp Leu Pr o G y Ar g Al a G u Al a Pr o Asp Thr G n Val G u 160 Ar g Al a Val Leu Thr 225 Pr o Gy Thr Asn Leu Ar g 195 Asp Asp Ar g Ar g Al a 275 Al a 165 Asp Val Al a Val G u 245 Leu Thr Al a G u Val Gy SI e 230 Ser Ser Ser Lys Tr p Ser Val 215 Asp Asp Thr Val Gene Sequence 24-2-06.txt His His Phe Asp Phe Ile Lys 170 Ser Arg G y Al a Al a Ser Thr 185 190 G y Vet Al a G u G y Tyr G u 200 205 Pro Val IVet Ala IVet Arg Arg 220 Ala Arg Arg G y G u G n Pr o 235 Asp Leu Val G u Ile Asp Thr 250 Trp Arg y Leu Arg Ser Val 265 270 Trp lie Pro Cys G n Lys Leu 280 285 G u 175 Sy Thr Sy Al a Val 255 Leu <210> 531 <211> 1545 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1522) <223> RXC01693 <400> 531 aggagactgg ctggattatt ggctggtttt cttgggaaat gtcccaacaa ttgccagaac ccgaacacta ggcttgaacc cgt cat gggc at t aat cct a atg aat act gca cct IVet Asn Thr Ala Pro ttc Phe ccc Pr o gac Asp cct Pr o aaa Lys gca Al a ct c Leu caa G n ct c Leu ggc Sy gtt Val ctt Leu gct Al a gag G u t ac Tyr caa G n cag G n gat Asp gac Asp gaa G u agc Ser ttt Phe gcc Al a 75 ggc Sy ttc Phe gcc Al a ct c Leu ctt Leu t at Tyr cga Ar g gca Al a ccc Pr o ggg Sy 45 ct c Leu t cc Ser gcg Al a t ca Ser aac Asn ctt Leu gga Sy ggc Sy ctt Leu gcc Al a 15 cct Pr o gat Asp ct c Leu cac Hi s ctt Leu 95 ct g Leu gag G u ccg Pr o aac Asn caa G n ct c Leu acc Thr gt g Val tgg Tr p gag G u gga Sy gaa G u at g Net att SIle ctt Leu ccc Pr o cag G n gcc Al a gca Al a tta Leu cgc Ar g tta Leu ttt Phe cgc Ar g 100 gct gac ggc gta ctg cat gat Ala Asp y Val Leu His Asp atc cac ctc aaa Ile His Leu Lys gga tct gga cga acc Sy Ser y Arg Thr Page 677 Gene Sequence 24-2-06.txt caa G n cgc Ar g acc Thr 150 agc Ser cga Ar g tct Ser gt a Val aaa Lys 230 ct g Leu gga Gy ggc Gy gaa G u acc Thr 310 gcc Al a cga Ar g acg Thr t cc Ser 120 t ac Tyr t ca Ser gcc Al a gga Gy cac Hi s 200 gaa G u at t SIle ttc Phe act Thr gcg Al a 280 caa G n ct c Leu gaa G u gaa G u gag G u 360 gga Gy at c SIle gca Al a ggc Gy 170 ttc Phe gcg Al a t cc Ser cag G n cac Hi s 250 gac Asp ttt Phe at g let ct c Leu ct c Leu 330 gcc Al a ttc Phe gcc Al a t cc Ser gt a Val 155 gca Al a caa G n aac Asn gca Al a cgc Ar g 235 gga Gy tac Ty r agc Ser at c SIle ct g Leu 315 gt a Val act Thr cgt Ar g gat Asp gaa G u 140 at t SIle gt c Val t ac Tyr t at Tyr cca Pr o 220 caa G n gcc Al a ggc Gy t cc Ser ct c Leu 300 ggc Gy gaa G u gca Al a gaa G u gga Gy 125 gcg Al a agc Ser ctt Leu t cc Ser acg Thr 205 acc Thr gca Al a ct c Leu cca Pr o at c SIle 285 gga Gy gcc Al a ttc Phe ct g Leu ct g Leu 365 110 cgc Ar g at g et acc Thr gtt Val aac Asn 190 at c SIle ccc Pr o gac Asp aac Asn tgc Cys 270 gac Asp tgg Tr p aca Thr gat Asp ggc Gy 350 ct c Leu gcc Al a cat Hi s ggt Gy cga Ar g 175 at c SIle acc Thr gca Al a acc Thr aca Thr 255 gct Al a act Thr aac Asn cca Pr o gac Asp 335 ctt Leu t ac Tyr gtc ctt Val Leu gca ctt Al a Leu 145 agg aaa Arg Lys 160 gta gca Val Ala tct ggt Ser G y agg cat Arg Hi s act tat Thr Tyr 225 gtt gga Val G y 240 gac aac Asp Asn ttc atg Phe IVet tat ggt Tyr G y atg gcc let Al a 305 gac gaa Asp u 320 ctc tgc Leu Cys gac gag Asp u ct c cat Leu Hi s ggc Gy 130 ggt Gy at c SIle acc Thr ggc Gy ttc Phe 210 gt a Val aaa Lys acg Thr gag G u cgc Ar g 290 cgc Ar g ggc Gy gaa G u t ca Ser aac Asn 370 gt c Val ccc Pr o cga Ar g ct c Leu 180 gaa G u tcg Ser ct g Leu acc Thr at a SIle 260 t ac Tyr aaa Lys gt a Val aca Thr gca Al a 340 acc Thr gac Asp tta Leu acc Thr gga Gy 165 at t SIe ct a Leu ttg Leu ttt Phe agg Ar g 245 t cc Ser cgt Ar g ttt Phe gaa G u gca Al a 325 at c SIe ggc Gy at c SIe 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 acc acg ctg ctg cgc gca ctc acc gac aac acc gca cca ccg agt ggc Thr Thr Leu Leu Arg Ala Leu Thr Asp Asn Thr Ala Pro Pro Ser Gy Page 678 Gene Sequence 24-2-06.txt 375 380 385 ttt gaa gca ttc gtt cac gac tgg aaa acc caa gac cca gat Phe G u Ala Phe Val His Asp Trp Lys Thr G n Asp Pro Asp 390 395 400 gca atg cga gca gta aat cca ctt ttc att cca cgc aat cac Ala IVet Arg Ala Val Asn Pro Leu Phe lie Pro Arg Asn His 410 415 gaa gct gct ctc gca gac gca gtt gaa ggg aat cta gaa aag G u Ala Ala Leu Ala Asp Ala Val G u y Asn Leu G u Lys 425 430 435 gaa ctc ctc gct gct gtc acc aat cct ttt gat cca act gcg G u Leu Leu Ala Ala Val Thr Asn Pro Phe Asp Pro Thr Ala 440 445 450 gat gaa cta cgc ctg cca agc gaa gaa gga ttt gaa gaa gac Asp G u Leu Arg Leu Pro Ser G u G u G y Phe G u G u Asp 455 460 465 acc ttc tgc ggt acc taggacagat ggtggggcag acg Thr Phe Cys G y Thr 470 <210> 532 <211> 474 <212> PRT <213> Corynebacter i um gl ut ai cum at c SIle ct c Leu 420 ttc Phe ggc Gy t ac Tyr gaa G u 405 gt g Val cac Hi s ccc Pr o at g bet 1315 1363 1411 1459 1507 1545 <400> 532 IVet Asn Thr Ala Pro Phe Lys Leu G u 1 Pr o Leu Thr Pr o Phe Gy Gy Leu Leu 145 Lys Al a vet SIle Leu Pr o G n Al a Gy 115 Pr o Val G n Ser 5 Al a Asn Thr Thr Val Ser Thr Leu Thr Gy 165 I e Pr o Asp Pr o Lys 70 Al a Al a G n Ar g Thr 150 Ser Ar g Tr p Asp G u Al a Ser Asp Phe G u 135 Ar g Val Val G n Leu 40 Sy Val Leu Sy Ser 120 Tyr Ser Al a Gy Sy Al a Val Al a Sy Val 105 Ar g SIle Leu Pr o Ser 185 Al a 10 G u Tyr G n G n Asp 90 Leu Gy I e Al a Sy 170 Asp G u Ser Phe Al a 75 Sy Hi s Al a Ser Val 155 Al a Phe Al a Leu Leu Tyr Ar g Asp Asp G u 140 I le Val Al a Pr o Sy Leu Ser Al a SIle Sy 125 Al a Ser Leu Al a Pr o Asp Leu Hi s Leu Leu Al a Hi s Sy Ar g 175 Leu G u Pr o Asn G n Leu Lys Val Al a Ar g 160 Val Phe G n Tyr Ser Asn I e Ser 190 Page 679 Gene Sequence 24-2-06.txt G y G y lie G u Leu Ser G n His Leu Ala Asn Tyr Thr Ile Thr 205 Hi s Tyr 225 Sy Asn let Sy Al a 305 G u Cys G u Hi s Al a 385 Asp Ar g Leu Pr o G u 465 195 Pr o Ser Tr p Leu Ar g 275 Tyr Leu Met G n Asp 355 Pr o Pr o Asp Hi s Lys 435 Al a Asp Leu Phe Ar g 245 Ser Ar g Phe G u Al a 325 I le Sy SIle Sy G u 405 Val Hi s Pr o let Val Lys 230 Leu Sy Sy G u Thr 310 Al a Ar g Thr Thr Phe 390 Al a G u G u Asp Thr 470 Al a 215 Al a Sy G u Asp Asn 295 Leu G n Lys Val Thr 375 G u et Al a Leu G u 455 Phe 200 G u l Ie Phe Thr Al a 280 G n Leu G u G u G u 360 Leu Al a Ar g Al a Leu 440 Leu Cys Leu Leu Val Val 265 Lys Pr o Pr o Al a Phe 345 G n Leu Phe Al a Leu 425 Al a Ar g Sy Ser G n Hi s 250 Asp Phe let Leu Leu 330 Al a Phe Ar g Val Val 410 Al a Al a Leu Thr Al a Ar g 235 Sy Tyr Ser SIle Leu 315 Val Thr Ar g Al a Hi s 395 Asn Asp Val Pr o Pr o 220 G n Al a Sy Ser Leu 300 Sy G u Al a G u Leu 380 Asp Pr o Al a Thr Ser 460 Thr Al a Leu Pr o SIle 285 Sy Al a Phe Leu Leu 365 Thr Tr p Leu Val Asn 445 G u Al a Thr Thr 255 Al a Thr Asn Pr o Asp 335 Leu Tyr Asn Thr SIe 415 Sy Phe Sy <210> 533 <211> 1236 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1213) <223> RXC01703 <400> 533 gttagacaaa tgggtaaaca gagctgacct agcggaatcc gccatcaacg aaaggcattc Page 680 Gene Sequence 24-2-06.txt cgcgagggtt tggggtctgc ctcgaacaaa tcttgggttt gtg gca tgg cca Val Ala Trp Pro aac Asn cat Hi s gcc Al a aat Asn ct g Leu cca Pr o t cc Ser gtt Val ttg Leu gat Asp 150 cct Pr o ggt Sy acc Thr t cc Ser cgg Ar g 230 ggc G y aaa Lys ct a Leu cgc Ar g ggc G y ctt Leu t ca Ser act Thr t cc Ser 120 gag G u gac Asp ctt Leu tgt Cys act Thr 200 aaa Lys at g Met ggc G y aaa Lys tgc Cys cgc Ar g ct g Leu ctt Leu ccc Pr o gt g Val ggt Gy at g Met ctt Leu cgt Ar g 170 gaa G u gt g Val ttt Phe aac Asn ttg Leu 250 ct g Leu ct g Leu at c SIle acc Thr ggg G y 75 tcg Ser ct g Leu cct Pr o aaa Lys gt g Val 155 t cc Ser at t SIle gat Asp gca Al a tt c Phe 235 tt c Phe ttt Phe gt g Val agg Ar g tcg Ser cgt Ar g ttg Leu ccg Pr o gct Al a at c SIle 140 at g Met at c SIle gct Al a cac Hi s cac Hi s 220 cac Hi s aag Lys at c SIle gat Asp gac Asp 45 aat Asn gcc Al a gaa G u tgg Tr p gcg Al a 125 acc Thr gac Asp cac Hi s ct c Leu tgg Tr p 205 at c SIle ggc G y ggc Gy cac Hi s gct Al a acc Thr cgt Ar g gga G y caa G n cgt Ar g 110 at c l Ie gat Asp gga Gy ccg Pr o aaa Lys 190 tcg Ser cac Hi s gtt Val at t SIle tgg Tr p 15 gct Al a at c SIle t at Tyr aaa Lys aac Asn 95 t cc Ser at g Met tgg Tr p ttg Leu t at Tyr 175 ct g Leu gat Asp gct Al a at t SIle ttg Leu 255 cac Hi s cgt Ar g cgc Ar g t ac Tyr gt g Val 80 at c SIle ggc Gy at g Met at t SIle cgc Ar g 160 tgc Cys cgt Ar g gcc Al a gt g Val gat Asp 240 gt c Val 1 t ac Tyr cga Ar g ggc G y gac Asp cga Ar g gt c Val gaa Gu gcc Al a ttt Phe 145 at g let caa G n gag G u gat Asp gtt Val 225 tgg Tr p cgc Ar g tgg Tr p cgc Ar g at t SIle gac Asp aag Lys gat Asp acc Thr cgc Ar g 130 gac Asp cgc Ar g ggt G y cgc Ar g aag Lys 210 cag G n gac Asp t at Tyr tgg Tr p acc Thr tcg Ser aaa Lys gt g Val ggc Gy ttc Phe 115 acc Thr aac Asn at g Met gt c Val gca Al a 195 gca Al a gct Al a acc Thr tta Leu caa G n aca Thr gt g Val gct Al a cgc Ar g at t SIle 100 t ac Tyr gac Asp ct g Leu cac Hi s gcc Al a 180 ggc G y gaa G u gt g Val ggt G y gct Al a 260 t cc Ser gcg Al a aag Lys cgc Ar g tgg Tr p aca Thr gat Asp aac Asn cgt Ar g at c SIe gga Gy 165 att SIe ttg Leu gac Asp tcg Ser gac Asp 245 gat Asp Page 681 gtg gcc atc cgc ctg cct gac Val Ala lle Arg Leu Pro Asp 265 aag att gca gca cgc ctg gta Lys lie Ala Ala Arg Leu Val 280 cac cga ttg gaa gtt gat ggc His Arg Leu G u Val Asp G y 295 300 acg gat gca cgc ctg cca caa Thr Asp Ala Arg Leu Pro G n 310 315 agc gat ctg gtg agt gtt gtg Ser Asp Leu Val Ser Val Val 330 caa ttg tcc ggt tgg atg ctc G n Leu Ser Gy Trp IVet Leu 345 gaa ctg gaa aac aac ggc aat G u Leu G u Asn Asn G y Asn 360 tagccccgat agtgtatgtg ct g <210> 534 <211> 371 <212> PRT <213> Corynebacter i um gl utar Gene Sequence 24-2-06.txt gat tca cca acc aac cgg gaa Asp Ser Pro Thr Asn Arg G u 270 275 ctg gaa tcg gcg gaa agc gta Leu G u Ser Ala G u Ser Val 285 290 ctt ccg gta ttc gcc aca gac Leu Pro Val Phe Ala Thr Asp 305 aac ttt ggt ttg agt tcc tct Asn Phe G y Leu Ser Ser Ser 320 cgc gtg gat gaa cgt gat ctg Arg Val Asp G u Arg Asp Leu 335 atg gaa gca gca gcg aaa gtg et G u Ala Ala Ala Lys Val 350 355 agt tac acc ggt cgc t cc cga Ser Tyr Thr Gy Arg Ser Arg 365 370 acc Thr tgg Tr p tgg Tr p agt Ser t cc Ser 340 gcc Al a 931 979 1027 1075 1123 1171 1213 1236 <400> 534 Val Ala Trp Pro Ser Asn Ala Lys G u 1 Tyr Ar g Gy Asp Ar g Val G u Al a Phe 145 Tr p Thr Ser Lys Val Gy Phe 115 Thr Asn 5 Al a Lys Ar g Tr p Thr Asp Asn Ar g SIle Tyr Ar g Val Al a Lys Leu Pr o Asp 135 Gy Asp Asp Lys Al a Leu Gy 105 Asn Al a Gy Lys Leu 10 Cys Leu Arg lie Leu Thr Leu G y 75 Pro Ser 90 Val Leu Sy Pro IVet Lys Leu Val 155 Page 682 Val Ar g Ser Ar g Leu Pr o Al a SIle 140 vet Phe Ile Hi s Asp Asp Asn Al a G u Tr p Al a 125 Thr Asp Al a Thr Ar g Gy G n Ar g 110 l Ie Asp Gy Trp His Al a Ar g li e Arg Tyr Tyr Lys Val Asn I e Ser G y Net Net Trp Ile Leu Arg 160 Gene Sequence 24- 2- 06. txt Pro u Leu Val Ara Ser lie His Pro Net Arg Net His G y 165 Sn y Val Ala l Ie 180 G u Arg Ala G y Leu 195 Asp Lys Ala G u Asp 210 Val G n Ala Val Ser 225 Trp Asp Thr y Asp 245 Arg Tyr Leu Ala Asp 260 Asn Arg G u Thr Lys 275 Su Ser Val Trp Asn 290 Ala Thr Asp Trp Thr 305 Ser Ser Ser Ser Leu 325 Arg Asp Leu Ser Val 340 Ala Lys Val Ala G u 355 Arg Ser Arg 370 <210> 535 <211> 800 <212> DNA Tyr Cys 175 Al a Thr Leu 215 Lys Asp Al a SIle Ar g 295 Asp Asp Leu Leu Cys Thr 200 Lys bet Gy l Ie Al a 280 Leu Al a Leu Ser G u 360 Leu 185 Val Tyr Thr Gy Ar g 265 Al a G u Ar g Val Gy 345 Asn SIle Asp Al a Phe 235 Phe Pr o Leu Asp Pr o 315 Val bet Gy Al a Hi s Hi s 220 Hi s Lys Asp Val Gy 300 G n Val Leu Asn Leu Tr p 205 I le Gy Gy Asp Leu 285 Leu Asn Ar g Net Ser 365 Leu Asp Al a SIe Leu 255 Pr o Ser Val Gy Asp 335 Al a Thr <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> RXC02254 <400> 535 atc gcc gtt gcc gaa gaa ggc gga ttg Ile Ala Val Al a G u G u Gy y Leu 1 5 cgc ttc ggt gga cat ggt gcg cta gct Arg Phe G y G y His G y Ala Leu Al a 25 gtg atc gcg gcg ttg acc gac att ttg Val Ile Ala Ala Leu Thr Asp Ile Leu 40 gat caa atc gct caa ctc gct gga gcc Asp G n Ile Al a G n Leu Al a G y Al a tgg gaa aac ctc ctg cag cac Trp G u Asn Leu Leu G n His 10 ggt cac gcc ttg gga aac ctc G y Hi s Ala Leu G y Asn Leu ggc acc tcc cag cat gcg ctt G y Thr Ser G n Hi s Al a Leu aaa gga cgc atc atc ccg gta Lys y Arg lie lle Pro Val Page 683 Gene Sequence 24-2-06.txt tgt Cys gat Asp acc Thr ccg Pr o ctt Leu cca Pr o 145 gt g Val gaa G u gac Asp aat Asn cat Hi s 225 gat Asp cga Ar g gaa cct ttg G u Pro Leu cga gt c at g Arg Val Mbet ggg cag gtg G y G n Val 100 ccc gct gcc Pro Al a Al a 115 cca ggt t cc Pr o G y Ser atc gtt gat Ile Val Asp aac ctg acg Asn Leu Thr 165 cac at c cat His Ile Hi s 180 gtc att gtc Val Ile Val 195 gta gaa cga Val G u Arg gtc cag gct Val n Ala gca aag ct g Ala Lys Leu 245 cgt taaggagt Ar g gat Asp 70 cgt Ar g cga Ar g at c l Ie tgg Tr p gcc Al a 150 t cc Ser gt g Val gat Asp gct Al a gaa G u 230 tgt Cys ctt Leu caa G n cgc Ar g gag G u ttc Phe 135 ttg Leu gag G u ct c Leu gcc Al a gct Al a 215 gat Asp gca Al a gaa G u gtt Val gtt Val gcc Al a 120 t cc Ser gcg Al a cca Pr o cgc Ar g aag Lys 200 cgc Ar g ggc Gy gcg Al a gcg Al a cgt Ar g cga Ar g 105 at t l Ie tct Ser cag G n ggg Gy cag G n 185 aca Thr acc Thr cgt Ar g ttg gaa G u ggt Gy 90 at c SIle ct c Leu gt g Val aca Thr gag G u 170 cat Hi s ct g Leu ctt Leu ggt Gty ct g gt a Val 75 caa G n at t SIle gat Asp at t SIle aaa Lys 155 acc Thr gct Al a t cc Ser ggt Gy cga Ar g 235 t ca Ser gt g Val ccg Pr o gca Al a cca Pr o 140 gca Al a gcg Al a cga Ar g t ca Ser gca Al a 220 ttc Phe ggt Gy gcg Al a gac Asp gat Asp 125 cac Hi s acc Thr gga Gy aac Asn caa G n 205 gaa G u acc Thr ct a gac Leu Asp gt a gct Val Ala aat cca Asn Pro 110 ttg gtc Leu Val att ttg I e Leu aaa acc Lys Thr ttc tct Phe Ser 175 ctt cag Leu G n 190 acc gaa Thr G u gtc t cc Val Ser agt at t Ser Ile tct Ser gca Al a gaa G u acc Thr gtc Val gtg Val 160 gca Al a gtt Val cgc Ar g ttc Phe cac Hi s 240 gca agt ttt gct Ala Ser Phe Al a Leu Leu 250 gga gca G y Al a 255 ag gcgtgtcact gac <210> 536 <211> 259 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 536 I Ie Ala Val Al a G u G u G y y Leu Trp G u Asn Leu Leu G n His 1 5 10 Arg Phe G y G y His y Ala Leu Ala G y His Ala Leu G y Asn Leu 25 Val IIe Ala Ala Leu Thr Asp I I e Leu G y Thr Ser G n His Ala Leu Page 684 Gene Sequence 24-2-06.txt Sy Ala Lys y Asp Cys Asp Thr Pr o Leu Pr o 145 Val G u Asp Asn Hi s 225 Asp Ar g SIle G u Ar g Gy Pr o 115 Pr o SIle Asn Hi s Val 195 Val Val Al a Ar g G n Leu Met Val Al a Ser Asp Thr 165 Hi s Val Ar g Al a Leu 245 Leu Asp 70 Ar g Ar g l Ie Tr p Al a 150 Ser Val Asp Al a G u 230 Cys Al a Leu G n Ar g G u Phe 135 Leu G u Leu Al a Al a 215 Asp Al a G u Val Val Al a 120 Ser Al a Pr o Ar g Lys 200 Ar g Gy Al a Al a Ar g Ar g 105 l le Ser G n Gy G n 185 Thr Thr Ar g Leu Val 75 G n SIle Asp SIle Lys 155 Thr Al a Ser Gy Ar g 235 Al a Ar g Ser Val Pr o Al a Pr o 140 Al a Al a Ar g Ser Al a 220 Phe Ser Gy Al a Asp Asp 125 Hi s Thr Gy Asn G n 205 G u Thr Phe Ile Ile Pro Val Asp Al a Pr o Val Leu Thr Ser 175 G n G u Ser SIle Sy 255 Ser Al a G u Thr Val Val 160 Al a Val Ar g Phe Hi s 240 Al a <210> 537 <211> 1059 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1036) <223> RXC02255 <400> 537 gtgtgcagcg ttgctggcaa gttttgctgg agcacgaaag cgttaaggag taggcgtgtc actgacgagt gatatcaaac aagaattggc gcaggtccat gtg gcc aaa aac agt 115 Val Ala Lys Asn Ser 1 gtt cgt gct gcg gaa gtg tct gca att tta agg ttt gct ggt gag atg 163 Val Arg Ala Ala u Val Ser Ala lie Leu Arg Phe Ala y G u IVet 15 caa gct gtc ggc ggc aag ctg gtc atc gaa gca aat ttg gac agc atg 211 G n Ala Val y y Lys Leu Val Ile G u Ala Asn Leu Asp Ser IVet Page 685 Gene Sequence 24- 2- 06. txt 30 caa gtc ggt atg agg ctt cag gag ttt atc caa ggt ttg tac aac tct 259 Sn Val Gy IVet Arg Leu n u Phe lie On y Leu Tyr Asn Ser 45 cga gtc gat gtg cac acc gtg aac ccg act gtg agc agg aaa acg cca 307 Arg Val Asp Val His Thr Val Asn Pro Thr Val Ser Arg Lys Thr Pro 60 cgg tat ttg gtg cgc atc att gac aat gcc gat gaa att gcg cga cgc 355 Arg Tyr Leu Val Arg Ile Ile Asp Asn Ala Asp u Ile Ala Arg Arg 75 80 acc gga ctg gtc acc agg tct gga cat gtg gtt aaa ggt cta gcg cct 403 Thr G y Leu Val Thr Arg Ser G y His Val Val Lys G y Leu Ala Pro 95 100 tct gtg gtc agc gga aca atc agt gac gct gaa gct gca tgg cgc ggt 451 Ser Val Val Ser y Thr lie Ser Asp Ala G u Ala Ala Trp Arg y 105 110 115 gcg ttt cta gcc aat gga tct tta agt gat cca ggt cgt tcc tct tcg 499 Ala Phe Leu Ala Asn G y Ser Leu Ser Asp Pro Gy Arg Ser Ser Ser 120 125 130 ttg gag gtg ttg tgt cct ggt caa gaa tca gca ttg gca ctg gtt gga 547 Leu G u Val Leu Cys Pro G y n G u Ser Ala Leu Ala Leu Val y 135 140 145 tgt gcg cga aga att ggg atc gcg gcg aaa acg aaa gat tct cga gga 595 Cys Ala Arg Arg lie Gy lie Ala Ala Lys Thr Lys Asp Ser Arg y 150 155 160 165 ttt gat cgc gtc aat gtt cgt gat gcg gaa gca att ggg gca ctg ctc 643 Phe Asp Arg Val Asn Val Arg Asp Ala G u Ala lle G y Ala Leu Leu 170 175 180 act cga atg ggt gcc cag aaa act cgc atg ttg tgg gaa gaa aaa cgc 691 Thr Arg Iet G y Al a n Lys Thr Arg IVet Leu Trp G u G u Lys Arg 185 190 195 atc aag cgg gaa agt cga act ccg caa acc ggg ttg gcc aac ttc gac 739 Ile Lys Arg u Ser Arg Thr Pro G n Thr y Leu Ala Asn Phe Asp 200 205 210 gat gcc aat ctg cgc agg tca gcc cga gca gca gtt gcc gct gca gcg 787 Asp Ala Asn Leu Arg Arg Ser Ala Arg Ala Ala Val Ala Ala Ala Ala 215 220 225 agg gta gaa cgc gcc atg aag att ctt ggt gat gat gtt cct gag cat 835 Arg Val G u Arg Al a Iet Lys Ile Leu y Asp Asp Val Pro G u His 230 235 240 245 ttg gct gag gct gga cag ctg cgt gtg cag cac cgt cag gca tcg ttg 883 Leu Ala G u Ala G y G n Leu Arg Val G n His Arg G n Ala Ser Leu 250 255 260 gag gag ttg ggc cgg ttg gct gat cct caa atg acc aag gat gct gtg 931 G u G u Leu y Arg Leu Ala Asp Pro G n Iet Thr Lys Asp Ala Val 265 270 275 gcc ggt cgt att cgt cgt ctt ttg acg atg gca gat aag cgc gcc gaa 979 Al a G y Arg I e Arg Arg Leu Leu Thr IVet Al a Asp Lys Arg Al a G u 280 285 290 gat ctg aag att cct gat aca aat tct gtt gtg acg gaa gat ttg ttg 1027 Asp Leu Lys lie Pro Asp Thr Asn Ser Val Val Thr G u Asp Leu Leu Page 686 Gene Sequence 24-2-06.txt 295 300 305 gaa gaa att tagatgattg aagcctaaaa acg G u Gu lie 310 <210> 538 <211> 312 <212> PRT <213> Corynebacter i um gl ut ai cum 1059 <400> 538 Val 1 Phe Asn Gy Ser G u Lys Al a Gy Leu 145 Lys SIle Tr p Leu Val 225 Asp Ar g Thr Ala Lys Asn Ser Val Arg Ala Ala Al a G y G u Leu Asp Ser Leu Tyr Asn Arg Lys Thr I e Al a Ar g G y Leu Al a 100 Al a Tr p Ar g 115 Arg Ser Ser 130 Ala Leu Val Asp Ser Ar g G y Al a Leu 180 G u G u Lys 195 Al a Asn Phe 210 Al a Al a Al a Val Pro G u G n Al a Ser 260 Lys Asp Al a 275 5 Met Met Ser Pr o Ar g Pr o Sy Ser Sy Sy 165 Leu Ar g Asp Al a Hi s 245 Leu Val Al a Val Val 55 Tyr Sy Val Phe G u 135 Al a Asp Ar g Lys Al a 215 Val Al a G u Sy Sy 25 vet Val Val Val Ser 105 Al a Leu Ar g Val Sy 185 G u Leu Ar g Al a Sy 265 I le Val Lys Leu Thr I e 75 Ar g Thr Sy Pr o Sy 155 Val G n Ar g Ar g et 235 G n Leu Ar g Ser Leu G n Val I e Ser SIle Ser Sy 140 I le Ar g Lys Thr Ser 220 Lys Leu Al a Leu Al a Val G u Asn Asp Sy Ser Leu 125 G n Al a Asp Thr Pr o 205 Al a SIle Ar g Asp Leu 285 I e I e Phe Pr o Asn Hi s Asp 110 Ser G u Al a Al a Ar g 190 G n Ar g Leu Val Pr o 270 Thr Leu Su I e Thr Al a Val Al a Asp Ser Lys G u 175 Met Thr Al a Sy G n 255 G n bet Ar g Al a G n Val Asp Val G u Pr o Al a Thr 160 Al a Leu Sy Al a Asp 240 Hi s bet Al a Asp Lys Arg Ala G u Asp Leu Lys lie Pro Asp Thr Asn Ser Val Val Page 687 Gene Sequence 24-2-06.txt 290 295 300 Thr G u Asp Leu Leu G u u le 305 310 <210> 539 <211> 681 <212> DNA <213> Corynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(658) <223> RXC02435 <400> 539 tcatgaatgt agaacggatt tatcgacaag cgccagcaag tacgttgatc tctttagcca tcatcgctgt gtacgcagtg cggccat t ca atcgagatca ggt Gy gat Asp cac Hi s ct g Leu at g et gat Asp at g Net gca Al a acc Thr tta Leu 150 at t l Ie acc Thr ggt Sy ggc G y aga Ar g ttt Phe t at Tyr at t SIle ttg Leu 120 gtt Val act Thr gt g Val at c I e ttt Phe ggg G y at t SIle ggc G y ccg Pr o gt g Val ctt Leu ct g Leu cca Pr o at t SIle 170 ggt Gy ggt G y cac Hi s gaa Gu ggt G y 75 aca Thr ggg Gy at c SIle tgg Tr p at g bet 155 ggt Gy gac Asp cca Pr o at g Met cga Ar g at t SIle gca Al a ctt Leu gcc Al a gga Gy 140 gtt Val ttt Phe gcg Al a ct g Leu ct g Leu 45 gac Asp ggt G y ggt G y ttt Phe at c l Ie 125 cac Hi s aaa Lys gct Al a tgg Tr p cgt Ar g ttg Leu ttc Phe gcg Al a gct Al a gt g Val 110 aac Asn ctt Leu gcg Al a gt a Val at t I e 15 gcc Al a aac Asn ggt G y tct Ser t cc Ser 95 tta Leu at c SIle gga Gy aaa Lys gtt Val 175 ct g Leu at c l Ie ctt Leu tct Ser gca Al a 80 ggc G y aga Ar g gcc Al a ggt Gy act Thr 160 gt g Val gt g Val 1 t ac Tyr gga G y gt g Val gcg Al a gct Al a gcc Al a agc Ser t at Tyr ttg Leu 145 caa G n gct Al a gca Al a gga G y ttg Leu ctt Leu gt c Val at t l Ie gcg Al a acc Thr 130 at c SIle aga Ar g gct Al a ccg Pr o at g let ttg Leu ttc Phe at c SIle t ac Tyr gat Asp 115 ttg Leu act Thr aca Thr gt c Val ct g Leu ttc Phe tgg Tr p act Thr tgg Tr p gcc Al a 100 at c SIle at g let gga Gy cgg Ar g at t SIle 180 aca gac aac Thr Asp Asn ctg Leu at g let ctg Leu ttg Leu gcg Al a at g let at g let cga Ar g t cc Ser gct Al a tgg Tr p 165 ct a Leu gga att gac cgg gtg tagacacatt ccgcccattg ccc Page 688 Gene Sequence 24-2-06.txt Gy Ile Asp Arg Val 185 <210> 540 <211> 186 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 540 Val Thr Asp 1 Tyr Gy Val Al a Al a Al a Ser Tyr Leu 145 G n Al a Pr o et Leu Phe l Ie Tyr Asp 115 Leu Thr Thr Val Asn Leu G y 5 Leu IVet Asp Phe Leu Hi s Trp Leu Leu Thr Al a Vbet 70 Trp IVbt Asp Al a [et Vbet 100 I e Ar g Al a Iet Ser Thr G y Al a Leu 150 Arg Trp Ile 165 Ile Leu G v Ser Asp SIle Gy Tyr Pr o Al a Pr o Asn 135 I e I e I e Ser Gy 25 Pr o G u Val Ser Leu 105 I le Ser Tr p Leu Ar g 185 Gy Gy Hi s G u Gy Thr Gy I e Tr p Net 155 Gy Asp Pr o Net Ar g I le Al a Leu Al a Gy 140 Val Phe Al a Leu Leu Asp Gy Gy Phe l Ie 125 Hi s Lys Al a Tr p Ar g Leu Phe Al a Al a Val 110 Asn Leu Al a Val I e Al a Asn Gy Ser Ser Leu SIe Gy Lys Val 175 <210> 541 <211> 681 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(658) <223> FRXA02435 <400> 541 tcatgaatgt agaacggatt tatcgacaag cgccagcaag tacgttgatc tctttagcca tcatcgctgt gtacgcagtg cggccattca atcgagatca gtg aca gac aac ctg 115 Val Thr Asp Asn Leu 1 ggt tca acc agt atc ggt gac gcg tgg att ctg tac gca ccg ctg atg 163 Gy Ser Thr Ser Ile Gy Asp Ala Trp lie Leu Tyr Ala Pro Leu IVet 15 Page 689 Gene Sequence 24-2-06.txt gat gat ggt Asp Asp G y cac Hi s ct g Leu at g bet gat Asp at g Met gca Al a acc Thr tta Leu 150 at t l Ie gga Gy ggc Gy aga Ar g ttt Phe t at Tyr at t SIle ttg Leu 120 gtt Val act Thr gt g Val gac Asp ggc ttt ggt G y Phe G y ccc ggg cac Pro G y Hi s gaa att gaa G u lie u gtg ggc ggt Val Gy y 75 tca ccg aca Ser Pro Thr ttg gtg ggg Leu Val G y 105 atc ctt atc Ile Leu Ile tct ctg tgg Ser Leu Trp t gg cca at g Trp Pro Ibet 155 ctc att ggt Leu Ile G y 170 cgg gtg tage Arg Val 185 cca ct g Pro Leu at g Mbt cga Ar g at t SIle gca Al a ctt Leu gcc Al a gga Gy 140 gtt Val ttt Phe ct g Leu 45 gac Asp ggt Gy ggt Gy ttt Phe at c l Ie 125 cac Hi s aaa Lys gct Al a cgt Ar g 30 ttg Leu ttc Phe gcg Al a gct Al a gt g Val 110 aac Asn ctt Leu gcg Al a gt a Val gcc Al a aac Asn ggt Gy tct Ser t cc Ser 95 tta Leu at c SIle gga Gy aaa Lys gtt Val 175 ctt Leu tct Ser gca Al a 80 ggc Gy aga Ar g gcc Al a ggt Gy act Thr 160 gt g Val gt g Val gcg Al a gct Al a gcc Al a agc Ser t at Tyr ttg Leu 145 caa G n gct Al a ttg Leu ctt Leu gt c Val at t l Ie gcg Al a acc Thr 130 at c SIle aga Ar g gct Al a ttg Leu ttc Phe at c SIle t ac Tyr gat Asp 115 ttg Leu act Thr aca Thr gt c Val tgg Tr p act Thr tgg Tr p gcc Al a 100 at c SIle at g bet gga Gy cgg Ar g at t SIle 180 atc gga gga atg ttc ctg I e Gy Gy IVet Phe Leu ttg Leu gcg Al a at g bet at g bet cga Ar g t cc Ser gct Al a tgg Tr p 165 ct a Leu acacatt ccgcccattg ccc <210> 542 <211> 186 <212> PRT <213> Corynebact <400> 542 Val Thr Asp Asn 1 Tyr Ala Pro Leu G y G y et Phe Val Leu Leu Trp Ala Leu Phe Thr Al a Val I le Tr p er i um gl ut ai cum Leu G y Ser Thr Ser 5 IVet Asp Asp G y y 25 Leu His lie G y Pro 40 Leu Leu Gy Arg G u Al a Net Tyr Phe Val 70 Iet Asp Pro Tyr Ser lie G y 10 Phe G y I G y Hi s I Ile Gu G y Gy yI 75 Pro Thr 90 Page 690 Al a Leu Leu Asp Gy Gy Tr p Ar g Leu Phe Al a Al a I e Al a Asn Gy Ser Ser Leu SIe Leu Ser Al a Gy Al a Ser Tyr Leu 145 G n Al a Tyr Asp 115 Leu Thr Thr Val let Ar g Ser Al a Tr p 165 Leu Met Al a Thr Leu 150 I e Gy Gene Sequence 24- 2- 06. txt Ile Leu Val y Leu Phe Val Leu Arg 105 110 Leu Ile Leu Ile Ala lie Asn Ile Ala 120 125 Val Ser Leu Trp G y His Leu G y G y 140 Thr Trp Pro lMt Val Lys Ala Lys Thr 155 160 Val Leu lie y Phe Ala Val Val Val 170 175 Asp Arg Val 185 <210> 543 <211> 1141 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1111) <223> RXC03216 <400> 543 gctgtatcac agtcagctgc agcaggtggc gaaaccgttg atccctgcaa acaactagaa actattcaga aagcatcacc cggtcccagc ggctgctctg atg aaa ctc ggt ctc IVet Lys Leu G y Leu t ac Tyr at c SIle ttg Leu gat Asp at c SIle at c l Ie gag G u ggc G y gcg Al a gct Al a gca Al a cgc Ar g ggc G y gac Asp ct c Leu cca Pr o 120 ttc Phe ggt Gy cac Hi s gaa Gu aac Asn cat Hi s caa G n gcg Al a cac Hi s ct c Leu at c SIle tt c Phe tgc Cys 75 gcc Al a aac Asn aag Lys gac Asp acc Thr ccg Pr o ct c Leu aac Asn gaa G u cgt Ar g t ac Tyr cgc Ar g gga Gy acc Thr 45 ggg G y ggc G y gac Asp gt g Val acc Thr 125 acc Thr at t SIle at c SIle at t SIle aac Asn at t SIle gt c Val 110 aac Asn ct g Leu 15 gaa G u gat Asp ttc Phe ccg Pr o cga Ar g 95 acc Thr tgg Tr p cca Pr o ct c Leu gac Asp gaa G u ctt Leu 80 cgt Ar g at g bet gtt Val 1 gaa G u aac Asn at c SIle ggc G y cac Hi s t cc Ser tct Ser gt c Val gcg Al a acc Thr ct g Leu acc Thr ccc Pr o at c SIle ggt G y gca Al a gga Gy agc Ser gt c Val aag Lys ct c Leu 100 cct Pr o gcc Al a ttt Phe gat Asp gac Asp gcg Al a gca Al a ggt G y aac gcg tgg aac Asn Ala Trp Asn 130 Page 691 Gene Sequence 24-2-06.txt t cc Ser ttc Phe 150 gct Al a cat Hi s gat Asp gat Asp gtt Val 230 cga Ar g tgg Tr p ggc Gy cac Hi s t ca Ser 310 gag G u gcc Al a cgc Ar g gag Su ct c Leu t ca Ser 200 ct c Leu aag Lys ctt Leu aat Asn ggt Gy 280 gt c Val ggt Sy aac Asn ttg gat Leu Asp gag acc G u Thr ctg cac Leu Hi s 170 at c gat I e Asp 185 cac ct g Hi s Leu ggc gag G y G u gaa t gg Su Trp gac cca Asp Pro 250 gaa t gg Su Trp 265 cat gac His Asp gat cca Asp Pro cgc gaa Arg u aag gca Lys Al a 330 gtc ctt Val Leu 140 gac cgc Asp Arg 155 cca cag Pro G n ctc acc Leu Thr ttc tgg Phe Trp ct c at c Leu II e 220 gct cag Al a G n 235 tcc gaa Ser G u cca aag Pro Lys gtt gct Val Ala aac at g Asn IVet 300 gaa ggc OuGy 315 ct c gaa Leu G u t ac caa t gg gat Tyr G n Trp Asp 145 gcc gca gat cac Ala Ala Asp Hi s 160 atc gtg ttc aac lie Val Phe Asn 175 gcc acc cac gtg Ala Thr His Val 190 cag atg gac cca G n IVet Asp Pro cac gcc gcc gcc Hi s Al a Al a Al a 225 aac ggt gtg ctg Asn y Val Leu 240 cgc acc aac ttg Arg Thr Asn Leu 255 tct gct tgg gat Ser Ala Trp Asp 270 tgg acc gaa ttc Trp Thr G u Phe gtc aac atc gaa Val Asn lle u 305 aac gaa gcc gct Asn u Al a Al a 320 atc gca gct gaa Ile Ala Ala u ggc G y t cc Ser ggc G y at c SIle 210 aaa Lys gac Asp ggc Gy ttc Phe ct c Leu 290 cac Hi s aag Lys aaa gt g Lys Val 165 gac gtg Asp Val 180 gaa ct g G u Leu gtg att Val lie gtg cga Val Arg agc ttc Ser Phe 245 gac gag Asp u 260 gct ctg Al a Leu gca ctt Al a Leu gat gtt Asp Val ct g at c Leu II e 547 595 643 691 739 787 835 883 931 979 1027 1075 1121 1141 gag tcc ctg gtt tct Su Ser Leu Val Ser 335 taaaaaaact acgcctgccc cgcaacgctt <210> 544 <211> 337 <212> PRT <213> Corynebacteri um gl ut arri cum <400> 544 IVet Lys Leu G y Leu Tyr Asn Ala lie Phe His Asp Arg Thr Leu Pro 1 5 10 G u Ala Leu Ala Ala lle Lys Ala Ala y Leu Thr G y I e G u Leu 25 Page 692 Gene Sequence 24- 2- 06. txt Ala Thr His Ile Pro Thr Ile Asn Thr G y Sy Phe Leu Pro Asp Asp SIle Gy Hi s Ser Ser Val Asp 145 Hi s Asn Val Pr o Al a 225 Leu Leu Asp Phe G u 305 Val Gy Asn Ar g Leu 115 Al a Al a Val Al a Val 195 Al a Asp Asn Gy Val 275 Ar g G u Asp Asp Al a Al a Gy Asn G u Val 165 Val Leu l Ie Ar g Phe 245 G u Leu Leu Val Asp SIle 70 l le G u Gy Ser Phe 150 Al a Hi s Asp Asp Val 230 Ar g Tr p Gy Hi s Ser 310 Al a Tyr Gy Ar g G u Al a 135 Tr p Leu Lys Al a Hi s 215 Asn Ar g Al a Ar g Ar g 295 Leu Asp Leu Lys Gy 105 Gy Leu G u Leu SIle 185 Hi s Gy G u Asp G u 265 Hi s Asp Ar g Phe Cys Al a Asn Lys Val Asp 155 Pr o Leu Phe Leu Al a 235 Ser Pr o Val Asn G u 315 Gy Gy Asp Val Thr 125 Asp Phe Asn Gy G n 205 Phe Leu Asn Asn Tyr 285 Leu Val SIle Asn SIle Val 110 Asn Tyr Al a SIle Al a 190 G n Hi s Asn Ar g Ser 270 Tr p Val Asn Phe Pr o Ar g Thr Tr p G n Al a Val 175 Thr bet Al a Gy Thr 255 Al a Thr Asn G u Ala Lys Val Leu lie G u Ala Asn Lys Ala Leu u u G uSer Leu Val 325 335 <210> 545 <211> 1416 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS Page 693 Gene Sequence 24-2-06.txt <222> (101)..(1393) <223> RXA02175 <400> 545 tgacatgcgc ttggcgcatc ccagttggta agaataaacg aagagttttt ttccgaacaa atatgtttga aagggatatc ggactacttc cgtaatccgg gtg gct act gat aac Val Ala Thr Asp Asn aac Asn at c SIle tct Ser t cc Ser cgt Ar g aac Asn gag G u gag G u at g Net gac Asp 150 cgc Ar g cgc Ar g gag G u at c SIle gct Al a gct Al a gct Al a act Thr gag G u cgc Ar g gtt Val cac Hi s ttc Phe 120 acc Thr ct g Leu at g let ggt Gy ttc Phe 200 cca Pr o cac Hi s ct g Leu gag G u ct g Leu aag Lys t at Tyr t ac Tyr ttt Phe t cc Ser gct Al a cca Pr o aag Lys 170 cct Pr o cgc Ar g at g et cag G n cac Hi s ggt Gy at c SIle at c SIle gac Asp 75 ct a Leu aac Asn cag G n t cc Ser ct c Leu 155 gtt Val tac Tyr at g bet gt c Val aac Asn t ac Tyr aac Asn act Thr acc Thr at c SIle ctt Leu gac Asp ttc Phe tcg Ser 140 gat Asp cca Pr o at g Net at g bet aag Lys 220 tgc Cys ccc ggt Pro G y aac ggt Asn y ttt gac Phe Asp 45 tac at c Tyr Ile gct gat Al a Asp atc aac Ile Asn gag att G u Ile 110 aac gtg Asn Val 125 gtt aac Val Asn gag gca G u Al a at g ct g IVet Leu tac cca Tyr Pro 190 ttc ggt Phe G y 205 gct ctg Al a Leu t cc acc Ser Thr gag G u gt c Val ggt Gy ggc Gy gct Al a gag G u cac Hi s cca Pr o ttg Leu ctt Leu 160 gcg Al a aac Asn cca Pr o aag Lys 1 ttc Phe ct g Leu t at Tyr gat Asp gag G u ct a Leu cac Hi s cgc Ar g tct Ser 145 gat Asp t ac Tyr t cc Ser acc Thr ct g Leu 225 gaa G u ggc Gy gt g Val gcg Al a aat Asn cca Pr o acc Thr gac Asp 130 acc Thr aag Lys gca Al a ct c Leu gag G u 210 ct c Leu at g Net aag Lys agc Ser gga Gy gcc Al a acc Thr ctt Leu 115 gct Al a t ac Tyr gca Al a cac Hi s aat Asn 195 cca Pr o at c SIle gac Asp at g bet act Thr at c SIe acc Thr cca Pr o 100 ctg Leu cac Hi s t ac Tyr acc Thr cgc Ar g 180 gcg Al a t ac Tyr ctg Leu tcc acc gtt cgt atg atc ggt Ser Thr Val Arg IVet iIe y Page 694 Gene Sequence 24-2-06.txt 230 235 240 245 tcc gca cag gcc aac atg ttt gtc tcc atc gct ggt ggc atc aac gct 883 Ser Ala G n Ala Asn IVet Phe Val Ser Ile Ala G y Gy ile Asn Ala 250 255 260 ctg tcc ggc cca ctg cac ggt ggc gca aac cag gct gtt ctg gag atg 931 Leu Ser G y Pro Leu His G y G y Ala Asn G n Ala Val Leu G u Met 265 270 275 ctc gaa gac atc aag agc aac cac ggt ggc gac gca acc gag ttc atg 979 Leu G u Asp I e Lys Ser Asn Hi s G y G y Asp Al a Thr G u Phe IVet 280 285 290 aac aag gtc aag aac aag gaa gac ggc gtc cgc ctc atg ggc ttc gga 1027 Asn Lys Val Lys Asn Lys G u Asp y Val Arg Leu IVet y Phe y 295 300 305 cac cgc gtt tac aag aac tac gat cca cgt gca gca atc gtc aag gag 1075 His Arg Val Tyr Lys Asn Tyr Asp Pro Arg Ala Ala lie Val Lys G u 310 315 320 325 acc gca cac gag atc ctc gag cac ctc ggt ggc gac gat ctt ctg gat 1123 Thr Al a Hi s G u lie Leu G u Hi s Leu G y G y Asp Asp Leu Leu Asp 330 335 340 ctg gca atc aag ctg gaa gaa att gca ctg gct gat gat tac ttc atc 1171 Leu Ala lie Lys Leu u u Ile Ala Leu Ala Asp Asp Tyr Phe Ile 345 350 355 tcc cgc aag ctc tac ccg aac gta gac ttc tac acc ggc ctg atc tac 1219 Ser Arg Lys Leu Tyr Pro Asn Val Asp Phe Tyr Thr y Leu lie Tyr 360 365 370 cgc gca atg ggc ttc cca act gac ttc ttc acc gta ttg ttc gca atc 1267 Arg Ala IVet y Phe Pro Thr Asp Phe Phe Thr Val Leu Phe Ala lie 375 380 385 ggt cgt ctg cca gga tgg atc gct cac tac cgc gag cag ctc ggt gca 1315 G y Arg Leu Pro G y Trp lie Ala His Tyr Arg G u G n Leu G y Ala 390 395 400 405 gca ggc aac aag atc aac cgc cca cgc cag gtc tac acc ggc aac gaa 1363 Ala y Asn Lys Ile Asn Arg Pro Arg G n Val Tyr Thr y Asn G u 410 415 420 tcc cgc aag ttg gtt cct cgc gag gag cgc taaatttagc ggatgattct 1413 Ser Arg Lys Leu Val Pro Arg G u G u Arg 425 430 cgt 1416 <210> 546 <211> 431 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 546 Val Ala Thr Asp Asn Asn Lys Ala Val Leu His Tyr Pro y y G u 1 5 10 Phe Gu IVbt Asp Ile lle uu Ala Ser u Gy Asn Asn Gy Val Val 25 Leu y Lys IVet Leu Ser G u Thr y Leu Ile Thr Phe Asp Pro y 40 Page 695 Tyr Val Ser Thr 03 y Ser Thr ene Sequence 24- 2- 06. txt Ou Ser Lys lieThr Tyr lieAsp Gy As p O31u Le u Hi s Ar g Ser 145 As p Tyr Ser Thr Le u 225 ValI G31y A a A a Le u 305 A a As p As p Thr ValI 385 G31y A a Thr Le u 115 A a Tyr A a Hi s As n 1 95 Pr o I Ie vb t I Ie Le u 275 O31u G31y ValI Le u Tyr 355 Le u Ph e Le u Ph e As p As p Pr o O31n ValI 1 65 A a Ar g O31u Hi s G31y 245 A a vb t vb t G31y O31u 325 As p I Ie Tyr I Ie Tyr O31u Le u As p A a 135 O31n Le u Ly s As n As p 215 As p A a Ser O31u Ly s 295 Ar g A a A a Ar g A a 375 Ar g G31y Ser Ly s 1 Ly s Le u As n A a A a 1 Le u I Ie O31u A a Pr o 265 li1e Ly s Tyr O31u Ly s 345 Le u G31y Pr o As p 75 Le u As n O31n Ser Le u 1 55 ValI Tyr vb t ValI As n 235 vb t Hi s Ser Ly s As n 315 Le u (31u Pr a Pr a Tr p 395 A a I Ie (31u As n 125 ValI (31u vb t Tyr Ph e 205 A a Ser ValI G31y Hi s 285 As p As p Hi s I Ie ValI 365 As p A a Le u G31y Ar g Ph e I Ie O31n A a 1 As p Tyr As p Ser I Ie 255 As n O31y ValI Ar g O31y 335 Le u Ph e Ph e Tyr A a O31u Hi s Pr a Le u Le u 1 A a As n Pr a Ly s Thr 240 A a O31n As p Ar g A a 320 O31y A a Tyr Thr Ar g 400 cOu On Leu Gy Ala Ala Gly Asn Lys lieAsn Arg Pro Arg 405 410 Page 696 O3 n Val1 415 ene Sequence 24- 2- 06. txt Tyr Thr OyAsnOu Ser Arg Lys LeuVal ProArg Ou uArg 420 425 430 <210O> 547 <21 1> 942 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 ODS <222> 101). (919) <223> RXA02621 <400> 547 at gt acct ga ct ggt gcgcg acact t cgct cact aaagt t gcagaccat a aggt ct gcct t t t cgcgt at t aat gagt ac t t taaagat t t cgct tgaag t gt Cys cca Pr o ggg G31y t cg Ser g at As p ac a Thr g at As p ag c Ser ggc G31y Le u 1 50 ac a Thr at t I Ie cca Pr o gca A a ggg G31y tt g Le u c ac Hi s gt t ValI ct c Le u cct Pr o 120 gag G31u g at As p gcc A a gcc A a at t lIe t cg Ser gt a ValI cct Pr a t tg Le u gt t ValI at t I Ie at t I Ie ct c Le u t cc Ser at g vb t 1 70 cat Hi s ct c Le u aag Ly s gac As p aaa Ly s gct A a 75 cct Pr a at c I Ie gct A a aca Thr aat As n 155 cac Hi s gcg A a tt c Ph e gcc A a cgt Ar g cga Ar g g ac As p aaa Ly s gcc A a gcg A a c ac Hi s 140 g aa G31u ct c Le u g at As p gca A a g at As p gag G31u 45 ac c Thr gt g ValI ct t Le u at g vb t g ac As p 125 ct c Le u ggc G31y c ac Hi s tt c Ph e cca Pr a at g vb t gt c ValI at t I Ie gag G31u ct t Le u at t I Ie 110 cct Pr a tt g Le u t cc Ser gcg A a c ac Hi s gct A a 15 gt c ValI gcc A a gt g ValI at g G31y 95 g aa G31u aaa Ly s gga G31y t ac Tyr gcg A a 1 75 gga G31y at c I Ie t ac Tyr ag a Ar g gt g ValI 80 ag c Ser ac c Thr gt c ValI ggc G31y cga Ar g 1 60 gcg A a at g t ct l'b t Ser 1 cgt gct Ar g Ala at t gat I I e As p agg aac Ar g As n acc gt a Thr Val aag t cc Lys Ser gt g cct Val Pr o cct cag Pro O31n gt t gga Val G31y 130 act cat Thr Hi s 145 gac acc Asp Thr aat ggg As n (1y gaa ctt 03 u Leu gag G31u tt g Le u at t I Ie ggg G31y ac g Thr gag G31u gct A a 115 at g vb t t ct Ser at g vb t aag Lys5 at c lIe g aa G31u ag a Ar g ccg Pr a g at As p g aa G31u 1 00 gca A a tt c Ph e ag g Ar g ag g Ar g tt c Ph e 1 at t le at t I Ie g at As p g aa G31u ag c Ser tt c Ph e tt a Le u ac c Thr t gg Tr p tt c Ph e ct t Le u 1 ac c Th r gat gaa gag ggc ctc tat tta Asp G31u 31u G31y Leu Tyr Leu Page 697 185 gaa gcg gtc gat gct gcg cgg G u Ala Val Asp Ala Al a Arg 200 cac ccc aag cag atc gag att His Pro Lys n lie u lle 215 220 aac cag ttg gag tgg gcg aag Asn G n Leu G u Trp Al a Lys 230 235 cca ggt gcg ttc aaa ctg gat Pro y Ala Phe Lys Leu Asp 250 tcg cag gcg cgg atg gtt att Ser n Ala Arg Met Val lie 265 aagcgttttt tcg <210> 548 <211> 273 <212> PRT <213> Cor ynebact er i um gl ut ar Gene Sequence 24-2-06.txt 190 195 act ggt ttc gct ggc acc gca tgc Thr G y Phe Al a G y Thr Al a Cys 205 210 gtt cgg aga gcc tat cgg cca gag Val Arg Arg Ala Tyr Arg Pro G u 225 aaa gtg gtg gag gaa gca gaa aac Lys Val Val G u G u Ala G u Asn 240 ggt cag atg att gat gct ccg ttg Gy n IVet lie Asp Ala Pro Leu 255 260 tcg cgt cag cct gct tgattagttc Ser Arg G n Pro Ala 270 <400> 548 Net Ser G u Leu 1 Arg Ala Gu lle Ile Asp Leu G u Arg Asn lie Arg Thr Val Gy Pro Lys Ser Thr Asp Val Pro G u G u 100 Pro G n Ala Ala 115 Val G y let Phe 130 Thr His Ser Arg 145 Asp Thr IVet Arg Asn G y Lys Phe 180 I e 5 I e Asp G u Ser Phe Leu Thr Tr p Phe Leu 165 Thr Cys y Pro Al a Lys Al a Gy 55 Pr o Leu Gy SIle Al a 135 Gy Ar g Asp Al a Gy 40 Leu Hi s Val Leu Pr o 120 G u Asp Al a Al a Al a 25 G u Asp Phe Net Asn 105 G n Asp G u Leu l Ie 185 Leu Lys Asp Lys Al a Pr o SIle Al a Thr Asn 155 Hi s Al a Phe Al a Ar g Ar g Asp Lys Al a Al a Hi s 140 G u Leu Asp Al a Asp G u Thr Val Leu Net Asp 125 Leu Sy Hi s Phe Pr o et Val SIle G u Leu SIle 110 Pr o Leu Ser Al a Hi s 190 Al a Val Al a Val bet Sy G u Lys Sy Tyr Al a 175 Asp Su y Leu Tyr Leu u Ala Val Asp Ala Ala Arg Thr y Phe Ala Page 698 ene Sequence 24- 2- 06. txt 195 200 205 (31yTh r Ala Cys I Ie Hi s Pro Lys O31n IlIe (1u IIe ValI Ar gAr gAla 210 215 220 Tyr Ar g Pr o (31u Ala Asn (31n Leu (31u Tr p Ala Lys Lys Val Val (31u 225 230 235 240 O31u Ala 1u Asn Hi s Pr o(1y Ala Phe Lys Leu Asp (1y (1n 'b t Ilie 245 250 255 Asp Ala Pr o Leu Ilie Ser O31n Ala Ar g I'b t Val Ilie Ser Ar g O31n Pr o 260 265 270 A a <210O> 549 <21 1> 2337 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (2314) <223> RXN00519 <400> 549 tttccatgcg gggctgaaac tgccaccata ggcgccagca t aggt agct g aacaaaagag cccat caacc aaggagact c at tagt agaa cact gtat tc at g gct aag at I'bt A a Lys II c at c e I Ie t gg Tr p ccg Pr o Ar g ct c Le u ct t Le u gct A a ggc G31y aaa cgc Ar g gt c ValI at t I Ie g aa O31u aag Lys5 gt t ValI g ac As p at c I Ie 120 g ac As p gca A a ct c Le u c ag O31n cct Pr o c ag O31n cca Pr o gca A a gaa O31u tt t Ph e gct A a aag Lys5 gaa O31u 75 ct c Le u gaa O31u cgc Ar g gca A a gct A a gga O31y gt a ValI gca A a aag Lys5 ct g Le u t ac Tyr ccg Pr a gct A a cgc Ar g 45 ggc O31y aac As n gct A a cct Pr a aac As n 125 ct g Le u ac c Thr at c I Ie aac As n at c I Ie gct A a g at As p 110 gct A a ct c Le u 15 gcg A a ct c Le u gca A a at t I Ie at t I Ie 95 aac As n gt t ValI gcg A a ggc O31y gcc A a ct c Le u aag Lys5 aag Ly s gcc A a aag Ly s 1 ac c Thr at t I Ie c ag O31n gca A a ct t Le u g aa O31u ac c Thr ggt (31y t ac Tyr gag O31u tt c Ph e g aa O31u cca Pr a ct g Le u ac c Thr t cc Ser 130 ct g Le u gag O31u gag O31u O31y at c I Ie g ac As p 1 00 gag O31u gt g ValI cca gtg ctg cgt gaa ggc aac tct gac cgc cgc gca cca atc gct gtc Page 699 Pro Val Leu Arg 135 3u 3y Asn Gene Sequence 24-2-06.txt Ser Asp Arg Arg Ala Pro I I e Al a Val aag Lys 150 gat Asp aac Asn cac Hi s ctt Leu ct g Leu 230 at c SIle cca Pr o gca Al a ggc G y gaa G u 310 gcc Al a gat Asp cac Hi s cca Pr o cgc Ar g 390 ttt Phe aag Lys aag Lys gca Al a 200 gaa G u gca Al a ttc Phe at c SIle t ac Tyr 280 gct Al a aag Lys gtt Val at c SIle tgg Tr p 360 t cc Ser aac Asn aag Lys aac Asn 170 at c SIle gac Asp gaa G u ctt Leu gca Al a 250 ggc G y gag G u at c l Ie gca Al a t cc Ser 330 gac Asp aaa Lys t ac Tyr gca Al a aag Lys 155 gtt Val at c SIle ggc Gy gtt Val ct c Leu 235 cac Hi s cac Hi s cag G n ct c Leu tt c Phe 315 gct Al a gct Al a gac Asp gct Al a tt c Phe 395 140 ttc Phe gca Al a ct c Leu acc Thr ct a Leu 220 gag G u ct g Leu gtt Val ct g Leu t cc Ser 300 gag G u cgc Ar g t cc Ser gac Asp ggc Gy 380 gat Asp cca Pr o acc Thr gac Asp gag G u 205 gac Asp cag G n aag Lys gt g Val ct c Leu 285 ggc G y aag Lys ggc Gy at g Net cag G n 365 gt c Val cca Pr o cac Hi s at g let gct Al a 190 acc Thr gga Gy gt c Val gcc Al a cgc Ar g 270 gca Al a ttg Leu ggc G y at c SIle cca Pr o 350 gag G u t ac Tyr acc Thr cgc Ar g gat Asp 175 gct Al a at c SIle acc Thr gct Al a acc Thr 255 gct Al a gct Al a gag G u ttg Leu acc Thr 335 gca Al a cag G n cag G n acc Thr at g let 160 gca Al a gat Asp ct c Leu gtt Val cgc Ar g 240 at g let t ac Tyr ggc G y t cc Ser gaa G u 320 aac Asn at g let gac Asp acc Thr at g 4et 400 145 ggc G y aac Asn gaa G u aag Lys ct g Leu 225 gca Al a at g tet ttc Phe ct c Leu ct g Leu 305 gac Asp ct g Leu at t SIle acc Thr gtt Val 385 ggt Gy gag G u gac Asp gtt Val gac Asp 210 t cc Ser aag Lys aag Lys gca Al a aac Asn 290 gac Asp ggc G y cat Hi s cgt Ar g ct g Leu 370 at c SIle acc Thr tgg Tr p ttc Phe cag G n 195 agc Ser gca Al a gca Al a gt c Val gac Asp 275 ggc G y aac Asn cca Pr o gt c Val acc Thr 355 gca Al a gaa G u gt c Val tct Ser cgc Ar g 180 at c SIle ct c Leu aag Lys gaa G u t cc Ser 260 gtt Val gaa G u ggc G y gac Asp cct Pr o 340 t cc Ser at c l Ie gac Asp cct Pr o gca Al a 165 cac Hi s aag Lys aag Lys gca Al a ggt G y 245 gac Asp ttc Phe aac Asn gaa G u ct g Leu 325 t cc Ser ggc G y at c SIe tgc Cys aac Asn 405 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 gtt ggt ctg atg gct cag aag gct gaa gag tac ggc tcc cat gac aag Page 700 Val y Leu lMt Ala G n Lys 410 Gene Sequence 24- 2- 06. txt Ala u u Tyr y Ser Hi s Asp Lys 420 acc Thr ggc Gy gca Al a gt c Val 470 cca Pr o ct g Leu gtt Val acc Thr ttc Phe 550 cct Pr o gct Al a gat Asp ct c Leu gac Asp 630 aag Lys ttc Phe cgc Ar g gtt Val 440 cag G n cgc Ar g cgc Ar g gac Asp gca Al a 520 tct Ser at c SIle at g et aag Lys ct c Leu 600 aac Asn gca Al a ggc G y gct Al a gaa G u at c SIle aag Lys cgt Ar g cac Hi s 490 gac Asp cag G n acc Thr gag G u ggc G y 570 gt c Val gag G u ggc G y gag G u at c SIle 650 gag G u gca Al a gag G u gat Asp ct c Leu 475 gac Asp acc Thr ct c Leu ggt G y ct g Leu 555 ggc G y cag G n tt c Phe aac Asn aag Lys 635 gac Asp ct c Leu gac Asp cac Hi s gcc Al a 460 t cc Ser cgc Ar g gag G u t cc Ser aac Asn 540 ggc G y gga G y cag G n ct c Leu acc Thr 620 ct g Leu aac Asn gct Al a ggt G y gac Asp 445 cca Pr o gga Gy aac Asn ggc Gy at c SIle 525 gtt Val acc Thr ct g Leu gtt Val gca Al a 605 aag Lys ct g Leu cgt Ar g gct Al a gt g Val 430 gtt Val at c SIle at g let ct g Leu ct g Leu 510 gac Asp ct g Leu tct Ser ttc Phe cag G n 590 ct g Leu gcc Al a aac Asn ggc G y cag G n 670 415 gtt Val gag G u cag G n cct Pr o gct Al a 495 gac Asp cgc Ar g cgt Ar g gca Al a gag G u 575 gaa G u gct Al a ggc G y gaa G u t cc Ser 655 acc Thr cag G n gca Al a gat Asp gca Al a 480 t cc Ser at c SIle at c SIle gac Asp aag Lys 560 acc Thr gaa G u gag G u gtt Val gag G u 640 cac Hi s gag G u gtt Val gac Asp 450 gt a Val ttc Phe gtt Val at c SIle cgt Ar g 530 aac Asn ct g Leu gct Al a cac Hi s ttc Phe 610 gct Al a t cc Ser tgg Tr p gca Al a t cc Ser tgg Tr p ctt Leu ttg Leu aag Lys 500 t cc Ser gag G u gac Asp gt c Val gga G y 580 cgt Ar g cac Hi s gct Al a t cc Ser acc Thr 660 ct g Leu 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 gct acc ttc gca cca gtc gca gaa gca ctg aac aca ggc gct gca gac Page 701 Gene Sequence 24- 2- 06. txt Ala Thr Phe Ala Pro Val Ala G u Ala Leu Asn Thr y Ala Ala Asp 680 685 690 atc gat gct gca ctg ctc gca gtt cag ggt gga gca act gac ctt ggt Ile Asp Ala Ala Leu Leu Ala Val Gn Gy Gy Ala Thr Asp Leu y 695 700 705 ggc tac tac tcc cct aac gag gag aag ctc acc aac atc atg cgc cca G y Tyr Tyr Ser Pro Asn G u G u Lys Leu Thr Asn Ile IVet Arg Pro 710 715 720 725 gtc gca cag ttc aac gag atc gtt gac gca ctg aag aag taaagtctct Val Ala G n Phe Asn G u Ile Val Asp Ala Leu Lys Lys 730 735 tcacaaaaag cgc <210> 550 <211> 738 <212> PRT <213> Cor ynebact eri um gl ut ai cum 2227 2275 2324 2337 <400> 550 Net 1 Thr SIle G n Al a Leu G u Thr Gy Al a 145 Gy Asn G u Lys Leu 225 Al a Lys II e Tyr Ser Leu Su Val u Phe Pro G u G u Leu G y Pro Asn li e Leu G n Asp 100 Thr Asp G u 115 Ser Al a Val 130 Pro lie Al a G u Trp Ser Asp Phe Arg 180 Val Gn lle 195 Asp Ser Leu 210 Ser Al a Lvs I e 5 Lys Thr Ar g G u Ser G n G u Asn Val Al a 165 Hi s Lys Lys Al a Trp Thr Arg Thr Asp 10 Pro Val Val G u Ala 25 Arg Asp lie Ser Leu 40 Leu Thr G u Asp G n 55 Leu Ala Lys Thr Pro 70 Al a Ser Val Pro G n 90 Sy Tyr Asp lie Pro 105 Lys Asp Ile Leu Ala 120 Pro Val Leu Arg G u 135 Lys Asn Phe Val Lys 150 Asp Ser Lys Thr Asn 170 Asn u Lys Ser lie 185 His Ile Ala Ala Asp 200 Leu Leu G u G y G u 215 Leu Asp Ala Phe Leu 230 G u Phe Al a Lys G u 75 Leu G u Ar g Sy Lys 155 Val SIle Sy Val Al a Al a Sy Val Al a Lys Leu Tyr Asn 140 Phe Al a Leu Thr Leu 220 Pr o Al a Ar g Sy Asn Al a Pr o Asn 125 Ser Pr o Thr Asp G u 205 Asp Leu Thr I le Asn SIle Al a Asp 110 Al a Asp Hi s et Al a 190 Thr Sy Leu Al a Leu Al a SIle l Ie Asn Val Ar g Ar g Asp 175 Al a SIle Thr Al a Sy Al a Leu Lys Lys Al a Lys Ar g bet 160 Al a Asp Leu Val Ar g 240 Leu G u n Val Ala 235 Page 702 ene Sequence 24- 2- 06. txt Al a Lys Al a 0 u vb t Ph e Le u Le u 305 As p Le u I Ie Th r ValI 385 O31y O31y ValI As n Tr p 465 ValI Le u O31n Ar g Ty r 545 vb t O31y ValI As p 275 O31y As n Pr o ValI Th r 355 Al a O31u ValI Hi s Ser 435 li1e Ly s Tr p O31u Le u 515 O31y Th r Ser (31y O31y 245 As p Ph e As n O31u Le u 325 Ser Oy le cys As n 405 Ly s As n Ar g Al a As p 485 Ty r Pr o As p Le u ValI 565 Ser Pr o Al a O31y O31u 310 Al a As p Hi s Pr a Ar g 390 ValI Th r O31y Al a ValI 470 Pr a Le u ValI Th r Ph e 550 Pr a Al a I Ie O31n Le u 295 li1e vb t ValI vb t As p 375 Ly s O31y Ph e As p cys 455 Th r O31u Al a O31u I Ie 535 Pr a Le u Pr a I I e Leu Phe Ser Al a Hi s Leu Lys Al a Thr l'bt I Ie Tyr 280 Al a Ly s ValI I Ie Tr p 360 Ser As n Le u Ar g ValI 440 O31n Ar g Ar g As p Al a 520 Ser I Ie vb t Ly s Ph e 265 O31y Al a Al a As n ValI 345 As n Ser O31y vb t I Ie 425 Le u ValI Ser Al a Hi s 505 Thr ValI Le u Al a Hi s 585 250 O31y O31u Al a Ser 330 As p Ly s Tyr Al a Al a 410 O31u I Ie Ly s Ar g Hi s 490 As p O31n Thr O31u O31y 570 ValI Hi s O31n Le u Ph e 315 Al a Al a As p Al a Ph e 395 O31n Al a O31u As p Le u 475 As p Thr Le u O31y Le u 555 O31y O31n ValI Le u 285 O31y Ly s O31y O31n 365 ValI Pr a Al a O31y As p 445 Pr a O31y As n O31y 525 ValI Thr Le u ValI Ar g 270 Al a Le u O31y Pr a 350 O31u Tyr Thr O31u ValI 430 ValI I Ie vb t Le u Le u 510 As p Le u Ser Ph e O31n 590 255 Al a Ty r Al a 03 y O3 u Ser Leu 03 u 320 Thr As n 335 Al a [Vb t O31n As p G3 n Thr Thr l'b t 400 O3 u Ty r 415 Val O31n O3 u Al a O31n As p Pr o Al a 480 Al a Ser 495 As p I I e Ar g I Ie Ar g As p Al a Lys 560 O3 u Thr 575 (31u (1u Asn His Leu Arg Trp Asp Ser Leu Oy Ou Phe Leu Ala Leu Ala Olu Page 703 Ser Leu 625 Lys Phe Asp Thr Al a 705 Asn Lys 595 Ar g Asp Pr o Leu Asp 675 Al a Asp Vet G u Leu Ar g 645 Lys Al a Asp Gy Pr o 725 Leu Asp 630 Lys Phe Al a SIle Gy 710 Val Asn 615 Lys Val Tr p Thr Asp 695 Tyr Al a Gene Sequence 24-2-06.txt 600 605 Asn Asn y Asn Thr Lys Al a 620 Ala Thr G u Lys Leu Leu Asn 635 Gy u lie Asp Asn Arg y 650 Ala Asp G u Leu Ala Ala G n 665 670 Phe Ala Pro Val Ala G u Ala 680 685 Ala Ala Leu Leu Ala Val G n 700 Tyr Ser Pro Asn G u G u Lys 715 Sn Phe Asn u Ile Val Asp 730 Gy G u Ser 655 Thr Leu Gy Leu Al a 735 <210> 551 <211> 1059 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (1)..(1059) <223> FRXA00521 <400> 551 ctc gac gct gct gat gaa gtt cag at c Leu Asp Ala Ala Asp u Val n Ile 1 5 acc gag acc atc ctc aag gac agc ctc Thr G u Thr Ile Leu Lys Asp Ser Leu 25 cta gac gga acc gtt ctg tcc gca aag Leu Asp G y Thr Val Leu Ser Ala Lys 40 gag cag gtc gct cgc gca aag gca gaa G u G n Val Ala Arg Al a Lys Ala G u 55 ctg aag gcc acc atg atg aag gtc t cc Leu Lys Ala Thr IVet IVet Lys Val Ser 70 gtt gtg cgc gct tac ttc gca gac gtt Val Val Arg Ala Tyr Phe Ala Asp Val ctg ctc gca gct ggc ctc aac ggc gaa Leu Leu Ala Ala G y Leu Asn G y G u 100 105 aag cac atc Lys His Il e 10 aag ctt ctt Lys Leu Leu gca ctg gac Ala Leu Asp ggt at c ct c G y I e Leu gac cca at c Asp Pro Ile ttc gca cag Phe Al a G n 90 aac ggc ct c Asn G y Leu Page 704 gca Al a gaa G u gca Al a ttc Phe at c SIle t ac Tyr gct Al a gct Al a ggc G y ttc Phe t cc Ser ttc Phe ggt G y gca Al a 110 gac Asp gaa G u ctt Leu gca Al a ggc G y gag G u at c SIe Gene Sequence 24-2-06.txt t cc Ser gag G u cgc Ar g 145 t cc Ser gac Asp ggc G y gat Asp aag Lys 225 gac Asp cac Hi s gcc Al a t cc Ser cgc Ar g 305 gag G u t cc Ser aac Asn ttg Leu 115 ggc G y at c SIle cca Pr o gag G u t ac Tyr 195 ace Thr gaa G u gt g Val gtt Val at c l Ie 275 at g Met ct g Leu ct g Leu gac Asp t cc Ser gaa G u aac Asn at g et 165 gac Asp acc Thr at g Net t ac Tyr cag G n 245 gca Al a gat Asp gca Al a t cc Ser at c SIle 325 at c SIle ctg gac Leu Asp gac ggc Asp y 135 ct g cat Leu Hi s 150 att cgt li e Ar g acc ctg Thr Leu gtt atc Val lile ggt acc G y Thr 215 ggc tcc G y Ser 230 gtt gtt Val Val aat gac Asn Asp tgg gta Trp Val gtg ttc Val Phe 295 ctc gtt Leu Val 310 cag at c Gn lle cgc cgt Arg Arg aac Asn 120 cca Pr o gt c Val acc Thr gca Al a gaa G u 200 gt c Val cat Hi s t cc Ser at c SIle aag Lys 280 tgg Tr p gag G u ct c Leu ggc G y ggc gaa gaa at c G y u u lie aag gct gca ttc Al a Al a Phe gac Asp cct Pr o t cc Ser at c l Ie 185 gac Asp cct Pr o gac Asp t cc Ser tgg Tr p 265 ctt Leu ttg Leu aag Lys t ac Tyr gag G u 345 gcc Al a gat Asp 155 cac Hi s cca Pr o cgc Ar g gtt Val acc Thr 235 ggc G y gca Al a gt c Val cca Pr o ct g Leu 315 gtt Val acc Thr Lys 125 gtt Val at c SIle tgg Tr p t cc Ser aac Asn 205 ct g Leu cgc Ar g gtt Val cag G n cgc Ar g 285 cgc Ar g gac Asp gca Al a tct Ser t cc Ser gac Asp aaa Lys 175 t ac Tyr gca Al a gct Al a gaa G u at c SIle 255 aag Lys cgt Ar g cac Hi s gac Asp cag G n 335 acc Thr gct Al a gct Al a 160 gac Asp gct Al a ttc Phe cag G n gca Al a 240 gag G u gat Asp ct c Leu gac Asp acc Thr 320 ct c Leu ggt G y 384 432 480 528 576 624 672 720 768 816 864 912 960 1008 1056 1059 <210> 552 <211> 353 Page 705 Gene Sequence 24-2-06.txt <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 552 Leu Asp Al a 1 Thr Leu G u Leu Val Leu Ser G u Ar g 145 Ser Asp Gy Asp Lys 225 Asp Hi s Al a Ser Ar g 305 G u Thr Gy Val Al a Ar g Al a Leu 115 Gy SIle Pr o G u Tyr 195 Thr G u Val Val l Ie 275 Let Leu Leu Al a Asp G u 5 Ile Leu Lys Thr Val Leu Al a Ar g Al a Thr IVet IVet 70 Al a Tyr Phe Al a y Leu 100 G u Ser Leu Leu G u Asp Thr Asn Leu 150 Ala IVet Ile 165 G n Asp Thr 180 G n Thr Val Thr et G y G u Tyr G y 230 Val G n Val 245 G u Al a Asn 260 Sn Asp Trp Pro Al a Val Al a Ser Leu 310 Asp Ile G n 325 Val Asp Ser Lys Lys Al a Asn Asp Sy 135 Hi s Ar g Leu SIle Thr 215 Ser Val Asp Val Phe 295 Val SIle G n Ser Al a 40 Al a Val Asp Sy Asn 120 Pr o Val Thr Al a G u 200 Val Hi s Ser SIle Lys 280 Tr p G u Leu SIle Leu 25 Lys G u Ser Val G u 105 Gy Asp Pr o Ser l Ie 185 Asp Pr o Asp Ser Tr p 265 Leu Leu Lys Tyr Hi s Leu Leu SIle Pr o Al a Sy G u Al a Asp 155 Hi s Pr o Ar g Val Thr 235 Sy Al a Val Pr o Leu 315 Val SIle Leu Asp Leu I le G n Leu SIle vet 140 Val bet Asp Lys Sy 220 Phe Asp Cys Thr G u 300 Al a G u Al a G u Al a Phe SIle Tyr Al a Lys 125 Val SIle Tr p Ser Asn 205 Leu Ar g Val G n Ar g 285 Ar g Asp Al a Al a Sy Phe Ser Phe Sy Al a 110 Al a Asn Val Asn Ser 190 Sy et SIle Leu Val 270 Ser Al a Hi s Thr Asp G u Leu Al a Sy G u l e Al a Ser Asp Lys 175 Tyr Al a Al a Su I e 255 Lys Ar g Hi s Asp G n 335 Ser lie Asp Arg lie Arg Arg Gy u Asp Thr lie Ser Val Thr y Page 706 Gene Sequence 24-2-06.txt 340 345 350 Asn <210> 553 <211> 1694 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (1)..(1671) <223> RXN02209 <400> 553 acc Thr 1 gac Asp cca Pr o acc Thr gct Al a act Thr cag G n at c SIle ggc G y tgg Tr p 145 t ac Tyr ct c Leu t cc Ser at c SIle t ac Tyr at g bet aac Asn gca Al a ggc Gy tct Ser 115 at c SIle aag Lys cgc Ar g ggc G y gtt Val 5 ct c Leu gac Asp aac Asn aac Asn gga Gy t ac Tyr acc Thr cgt Ar g at c SIle gac Asp 165 ggc G y gtt Val t cc Ser gac Asp gaa Gu gct Al a 70 cgt Ar g acc Thr aac Asn aag Lys tgt Cys 150 ct c Leu tgc Cys cct Pr o gag G u gct Al a ggt Gy t cc Ser cct Pr o at c SIle acc Thr gca Al a 135 gca Al a tgg Tr p acc Thr t cc Ser gca Al a gtt Val 40 ggc Gy tgg Tr p t cc Ser gac Asp tct Ser 120 gca Al a cca Pr o aag Lys acc Thr at c SIle aag Lys t cc Ser gga Gy gct Al a aag Lys cac Hi s 105 aac Asn gaa G u ggt G y gac Asp tgt Cys 185 gct Al a 10 gag G u gt a Val cag Gn ggc Gy cca Pr o 90 ggc Gy cca Pr o aag Lys t cc Ser ctt Leu 170 at t SIle gag G u ggc G y cag G n gac Asp cct Pr o t cc Ser 75 gt c Val at g bet t cc Ser ggc G y cag G n 155 gag G u ggt G y cac Hi s cct Pr o ttc Phe acc Thr gaa Gu ggc G y acc Thr gtt Val gt g Val ct c Leu 140 gtt Val gcc Al a aac Asn gac Asp aag Lys cgt Ar g t cc Ser ggc Gy gag G u gtt Val gca Al a at g bet 125 aag Lys gt c Val at g bet t cc Ser ct g Leu 205 cca Pr o gat Asp cct Pr o gt c Val ttg Leu t cc Ser gca Al a ggc G y aag Lys G y ttc Phe 175 cca Pr o gca Al a cag G n ctg Leu gca Al a gaa Gu gct Al a cca Pr o t cc Ser gct Al a cct Pr o t ac Tyr 160 t ac Tyr ctg Leu acc Thr cca gag gaa atc tcc gct gcg atc aac Pro u u lie Ser Ala Ala lie Asn Page 707 Gene Sequence 24-2-06.txt gca Al a gtt Val 225 at c SIle gac Asp gag Su gaa G u ct c Leu 305 t ac Tyr gca Al a tct Ser acc Thr t ac Tyr 385 ggc G y ggt Sy at c SIle gt c Val gct Al a 465 ttg Leu at g bet ggc G y gac Asp at c SIle 275 gac Asp gtt Val cgc Ar g acc Thr acc Thr 355 gca Al a t cc Ser ttc Phe t ac Tyr gac Asp 435 ggc G y ggc G y ggt aac cgt G y Asn Ar g 215 t ac ct g gca Tyr Leu Al a 230 atg gac ttc IVet Asp Phe 245 aac gac gt c Asn Asp Val gac acc atc Asp Thr Ile gca gat gt c Al a Asp Val 295 acc ggt gac Thr G y Asp 310 gca cct t ac Al a Pro Tyr 325 atc cag ggc lie Gn y gac cac at c Asp His Ile cag tac ttg G n Tyr Leu 375 ggt tcc agg G y Ser Arg 390 aac atc cgc Asn lie Ar g 405 cgc gac ttc Arg Asp Phe t cc gt c aac Ser Val Asn aag gag tac Lys G u Tyr 455 aac ct g ct c Asn Leu Leu 470 aac ttc gag gga Asn Phe G u G y cgt atc tcc cct Arg Ile Ser Pro t cc Ser gac Asp ttc Phe cag G n 280 ttc Phe acc Thr ttc Phe gca Al a t cc Ser 360 gat Asp cgt Ar g ct c Leu acc Thr t ac Tyr 440 ggc G y gga Gy cca Pr o ttc Phe ct g Leu 265 cag G n aag Lys ttc Phe gac Asp cgc Ar g 345 cct Pr o gag G u ggt G y cag G n cag G n 425 aag Lys acc Thr at t SIle at c SIle gag G u 250 aag Lys gca Al a ggt G y gag G u ggc G y 330 gtt Val gct Al a cac Hi s aac Asn aac Asn 410 gag G u gct Al a ggt G y cgc Ar g at g bet 235 aac Asn gac Asp at c l Ie gac Asp tgg Tr p 315 at g bet ct g Leu t cc Ser ggt G y cac Hi s 395 cag G n ggt G y gct Al a tct Ser gca Al a 475 at t SI e gct Al a tgg Tr p cgt Ar g 285 cag G n gag G u gt c Val aag Lys at t SIle 365 gaa G u gt c Val gtt Val cca Pr o at t l Ie 445 cgt Ar g at c l Ie gct Al a ctt Leu cct Pr o 270 gag G u tgg Tr p aac Asn gag G u ct c Leu 350 aag Lys cgc Ar g at g et gac Asp cag G n 430 ccg Pr o gac Asp acc Thr t ac Tyr gga G y 255 t cc Ser ctt Leu cag G n t cc Ser cca Pr o 335 ggc G y cca Pr o cac Hi s at g bet at c SIe 415 gcg Al a ct g Leu tgg Tr p gag G u 672 720 768 816 864 912 960 1008 1056 1104 1152 1200 1248 1296 1344 1392 1440 Page 708 ttc gag cgt att cac cgc tcc aac c Phe G u Arg lie His Arg Ser Asn L 485 ctg cag ttc cct gca ggc gaa tcc c Leu n Phe Pro Ala y Gu Ser 500 5 acc gag acc ttc gac atc acc gga c Thr G u Thr Phe Asp Ile Thr Gy L 515 520 act cct aag act gtc aag gtc acc g Thr Pro Lys Thr Val Lys Val Thr A 530 535 gtc gag ttc gac gca att tgt ccg c Val G u Phe Asp Ala lle Cys Pro 545 550 tactaccgcc acg <210> 554 <211> 557 <212> PRT <213> Cor ynebact eri um gl ut am cum Sequence 24-2-06. t xt t c atc ggt atg ggc gtt gtc cca eu II e G y Met G y Val Val Pr o 490 495 ac gag tcc ctg ggc ctt gac ggc is u Ser Leu Gy Leu Asp Gy ;05 510 tg acc gca ctt aac gag ggc gag .eu Thr Ala Leu Asn G u G y G u 525 ica acc aag gag aac ggc gac gtc l a Thr Lys G u Asn y Asp Val 540 at cga cac ccc agg tgaggctgac is Arg His Pro Arg 555 1488 1536 1584 1632 1681 1694 <400> 554 Thr G u Ser Thr Val Val Pro Ser lie 1 Asp Pr o Thr Al a Thr G n SIle Sy Tr p 145 Tyr Leu SIle Tyr Met Asn Al a Sy Ser 115 I le Lys Ar g Sy 5 Leu Asp Asn Asn Sy Tyr Thr Ar g SIle Asp 165 Sy G u Al a Gy Ser Pr o SIle Thr Al a 135 Al a Tr p Thr Al a Val 40 Gy Tr p Ser Asp Ser 120 Al a Pr o Lys Thr Lys Ser Gy Al a Lys Hi s 105 Asn G u Sy Asp Cys 185 Al a 10 G u Val Gn Gy Pr o 90 Gy Pr o Lys Ser Leu 170 I le Gy G n Asp Pr o Ser 75 Val Met Ser Sy G n 155 G u Sy Pr o Phe Thr Gu Sy Thr Val Val Leu 140 Val Al a Asn Lys Ar g Ser Gy G u Val Al a bet 125 Lys Val Met Ser Pr o Asp Pr o Val Leu Ser Al a Sy Lys Sy Phe 175 Pr o G n Leu Al a Gu Al a Pr o Ser Al a Pr o Tyr 160 Tyr Leu Pro G u G u lie Ser Ala Ala lie Asn G u His Asp Leu Thr Ala Thr 195 200 205 Page 709 Gene Sequence 24-2-06.txt Al a Val 225 I le Asp G u G u Leu 305 Tyr Al a Ser Thr Tyr 385 Gy Gy SIle Val Al a 465 Phe Leu Thr Thr Val 545 Leu vet Gy Asp I le 275 Asp Val Ar g Thr Thr 355 Al a Ser Phe Tyr Asp 435 Gy Gy Ar g Phe Thr 515 Lys Phe Gy Tyr vet 245 Asn Asp Al a Thr Al a 325 I le Asp G n Gy Asn 405 Ar g Ser Lys Asn Hi s 485 Al a Asp Val Al a Ar g 215 Al a Phe Val SIle Val 295 Asp Tyr Gy SIle Leu 375 Ar g Ar g Phe Asn Tyr 455 Leu Ser G u Thr Val 535 Cys Asn Phe G u G y Ser Asp Phe G n 280 Phe Thr Phe Al a Ser 360 Asp Ar g Leu Thr Tyr 440 Sy Sy Asn Ser Sy 520 Thr Pr o Pr o Phe Leu 265 G n Lys Phe Asp Ar g 345 Pr o G u Gy G n G n 425 Lys Thr SIle Leu Hi s 505 Leu Al a Hi s SIle G u 250 Lys Al a Gy G u Gy 330 Val Al a Hi s Asn Asn 410 G u Al a Gy Ar g SIle 490 G u Thr Thr Ar g bet 235 Asn Asp l Ie Asp Tr p 315 bet Leu Ser Gy Hi s 395 G n Sy Al a Ser Al a 475 Sy Ser Al a Lys Hi s 555 Ar g 220 Val G u SIle Ser Lys 300 Asp Pr o Al a Ser Val 380 G u Leu Al a Sy Ser 460 Val bet Leu Leu G u 540 Pr o Ile Ser Pro Asp I e Al a Tr p Ar g 285 G n G u Val Lys SIle 365 G u Val Val Pr o SIle 445 Ar g SIle Sy Sy Asn 525 Asn Ar g Al a Leu Pr o 270 G u Tr p Asn G u Leu 350 Lys Ar g bet Asp G n 430 Pr o Asp Thr Val Leu 510 G u Sy Tyr Sy 255 Ser Leu G n Ser Pr o 335 Gy Pr o Hi s bet SIe 415 Al a Leu Tr p G u Val 495 Asp Sy Asp Page 710 21> 55 ene Sequence 24- 2- 06. txt <211> 1682 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> ODS <222> (1659) <223> FRXA02209 <400> 555 gt t ValI ct c Le u g ac As p aac As n aac As n gga G31y t ac Ty r ac c Th r cgt Ar g at c I Ie 145 g ac As p ggc G31y t cc Ser ggt G31y cct Pr o gag O31u gct A a ggt G31y t cc Ser cct Pr o at c I Ie ac c Th r 115 gca A a gca A a t gg Tr p ac c Thr gcg A a 1 95 cgt Ar g at c le aag Ly s t cc Ser gga G31y gct A a aag Ly s c ac Hi s aac As n g aa O31u ggt G31y g ac As p 1 65 t gt cys aac As n tt c Ph e gct A a gag O31u gt a ValI cag O31n G31y 70 cca Pr o G31y cca Pr a aag Ly s t cc Ser 150 ct t Le u at t I Ie gag O31u gag O31u ggc G31y c ag O31n g ac As p cct Pr a t cc Ser gt c ValI at g vb t t cc Ser ggc G31y 135 c ag O31n gag (31u ggt G31y c ac Hi s gga G31y 215 cct Pr a tt c Ph e ac c Thr 40 g aa (31u G31y ac c Thr gt t ValI gt g ValI 120 ct c Le u gt t ValI gcc A a aac As n g ac As p 200 cgt Ar g aag Ly s cgt Ar g t cc Ser ggc G31y gag (31u gt t ValI gca A a 1 at g vb t aag Ly s gt c ValI at g vb t t cc Ser 1 ct g Le u at c I Ie cca Pr a g at As p cct Pr a gt c ValI tt g Le u 75 t cc Ser gca A a ggc G31y aag Ly s ggc G31y 1 55 tt c Ph e cca Pr a gca A a cct Pr a c ag O31n ct g Le u gca A a g aa O31u gct A a cca Pr a t cc Ser gct A a cct Pr a 140 t ac Tyr t ac Tyr ct g Le u ac c Thr g ac As p 220 g ac As p cca Pr a ac c Thr gct A a act Thr c ag O31n at c O31y 125 t gg Tr p t ac Tyr ct c Le u cca Pr a gca A a 205 gt t ValI at c lIe t ac Tyr at g vb t aac As n gca A a ggc O31y t ct Ser at c I Ie aag Ly s cgc Ar g ggc O31y 1 g aa O31u tt g Le u at g vb t ct t Le u ac c Thr gt t ValI t ac Tyr g aa O31u gag O31u t gc cys gca A a ac c Thr gca A a 1 tt c Ph e at c I Ie t cc Se r aac As n t ac ct g gca t cc cca at c at g gt c at t gct tac gca atc gct ggc acc Page 711 Tyr 225 at g bet aac Asn gac Asp gca Al a acc Thr 305 gca Al a at c SIle gac Asp cag G n ggt Sy 385 aac Asn cgc Ar g t cc Ser aag Lys aac Asn 465 cac Hi s Leu Ala Ser Pro Ile IVet 230 Gene Sequence 24- 2- 06. txt Val I e Ala Tyr Ala Ile Ala y Thr 235 240 ttc Phe gt c Val at c SIle 275 gt c Val gac Asp t ac Tyr ggc G y at c SIle 355 ttg Leu agg Ar g cgc Ar g ttc Phe aac Asn 435 t ac Tyr ct c Leu t cc Ser ttc Phe 245 ct g Leu cag G n aag Lys ttc Phe gac Asp 325 cgc Ar g cct Pr o gag Su ggt Sy cag G n 405 cag G n aag Lys acc Thr at t SIle ct c Leu 485 gag G u aag Lys gca Al a ggt G y gag G u 310 ggc G y gtt Val gct Al a cac Hi s aac Asn 390 aac Asn gag G u gct Al a ggt G y cgc Ar g 470 at c SIle aac Asn gac Asp at c l Ie gac Asp 295 tgg Tr p at g bet ct g Leu t cc Ser ggt G y 375 cac Hi s cag G n ggt Gy gct Al a tct Ser 455 gca Al a ggt Gy gaa G u at c SIle t cc Ser 280 aag Lys gac Asp cct Pr o gct Al a t cc Ser 360 gt g Val gag G u ct g Leu gct Al a ggc Gy 440 t cc Ser gtt Val at g bet gct Al a tgg Tr p 265 cgt Ar g cag G n gag G u gt c Val aag Lys 345 at t SIle gaa G u gt c Val gtt Val cca Pr o 425 at t SIle cgt Ar g at c SIle ggc Gy gga G y t cc Ser ctt Leu cag G n t cc Ser 315 cca Pr o ggc G y cca Pr o cac Hi s at g bet 395 at c SIle gcg Al a ct g Leu tgg Tr p gag G u 475 gt c Val cag G n acc Thr t ac Tyr gaa G u 300 acc Thr gt g Val gac Asp ggt G y gac Asp 380 cgc Ar g gca Al a ttc Phe gt c Val gca Al a 460 t cc Ser cca Pr o gac Asp gag G u gaa G u 285 ct c Leu t ac Tyr gca Al a tct Ser acc Thr 365 t ac Tyr ggc G y ggt G y at c SIle gt c Val 445 gct Al a ttc Phe ct g Leu cag G n gaa G u 270 gct Al a gat Asp at c SIle gt c Val gt c Val 350 cct Pr o aac Asn acc Thr ggc G y t ac Tyr 430 ttg Leu aag Lys gag G u cag G n gac Asp 255 at c SIe gac Asp gtt Val cgc Ar g acc Thr 335 acc Thr gca Al a t cc Ser ttc Phe t ac Tyr 415 gac Asp ggc G y ggc G y cgt Ar g ttc Phe 495 768 816 864 912 960 1008 1056 1104 1152 1200 1248 1296 1344 1392 1440 1488 1536 gca ggc gaa tcc cac gag tcc ctg ggc ctt gac ggc acc gag acc ttc Page 712 Gene Sequence 24-2-06.txt Ala G y u Ser His G u Ser Leu G y Leu Asp G y Thr G u Thr Phe 500 505 510 gac atc acc gga ctg acc gca ctt aac gag ggc gag act cct aag act Asp Ile Thr y Leu Thr Ala Leu Asn u G y G u Thr Pro Lys Thr 515 520 525 gtc aag gtc acc gca acc aag gag aac ggc gac gtc gtc gag ttc gac Val Lys Val Thr Ala Thr Lys G u Asn y Asp Val Val G u Phe Asp 530 535 540 gca att tgt ccg cat cga cac ccc agg tgaggctgac tactaccgcc Ala lie Cys Pro His Arg His Pro Arg 545 550 acg <210> 556 <211> 553 <212> PRT <213> Cor ynebact eri um gl ut ai cum 1584 1632 1679 1682 <400> 556 Val 1 Leu Asp Asn Asn Gy Tyr Thr Ar g SIle 145 Asp Gy Ser Gy Tyr 225 Val Pro Ser Ser G u Al E 2C Asp Al a Val Gu Gy G Al a Ser Tr r Arg Pro Ser Thr Ile Asp Asn Thr Ser 115 Lys Al a Al E 130 Cys Ala Prc Leu Trp Lys Cys Thr Thr 18C Al a Al a I I E 195 Asn Arg Asr 210 Leu Al a Ser I e 5 Lys Ser Gy Al a Lys Hi s Asn G u Gy Asp 165 Cys Asn Phe Pr o Al a Gy Pro Lys G n Asp Pr o 55 Ser Val bet Ser Gy 135 G n G u Gy Hi s Gy 215 \et Ar g Ser Gy G u Val Al a 105 vet Lys Val et Ser 185 Leu SIle SI e Arg Pro 10 Lys Asp Ile Pro Sy Val Ser Leu Al a Ser 90 l e Al a I e G y Ser Lys Asp y 155 G y Phe 170 G y Pro Thr Al a Ser Pro Al a Tyr 235 G n Leu Al a Gu Al a Pr o Ser Al a Pr o 140 Tyr Tyr Leu Thr Asp 220 Asp Pr o Thr Al a Thr G n SIle Sy 125 Tr p Tyr Leu Pr o Al a 205 Val I e Tyr bet Asn Al a Sy Ser SIe Lys Ar g Sy 175 G u Leu vet Ala l e Al a y Thr Page 713 Gene Sequence 24-2-06.txt IVet Asp Phe Asp Phe G u Asn u Al a Leu Gy G n Asp G n Asn Asp Al a Thr 305 Al a SIle Asp G n Sy 385 Asn Ar g Ser Lys Asn 465 Hi s Al a Asp Val Al a 545 Val SIle 275 Val Asp Tyr Sy SIle 355 Leu Ar g Ar g Phe Asn 435 Tyr Leu Ser G u Thr 515 Val Cys 245 Leu G n Lys Phe Asp 325 Ar g Pr o G u Sy G n 405 G n Lys Thr SIle Leu 485 Hi s Leu Al a Hi s Lys Al a Sy G u 310 Sy Val Al a Hi s Asn 390 Asn G u Al a Sy Ar g 470 I le G u Thr Thr Ar g 550 Asp l Ie Asp 295 Tr p let Leu Ser Gy 375 Hi s G n Sy Al a Ser 455 Al a Sy Ser Al a Lys 535 Hi s SIle Ser 280 Lys Asp Pr o Al a Ser 360 Val G u Leu Al a Sy 440 Ser Val et Leu Leu 520 G u Pr o Tr p 265 Ar g G n G u Val Lys 345 I le G u Val Val Pr o 425 I le Ar g SIle Sy Sy 505 Asn Asn Ar g Ser Leu G n Ser 315 Pr o Sy Pr o Hi s let 395 I le Al a Leu Tr p G u 475 Val Asp Sy Asp G u G u 285 Leu Tyr Al a Ser Thr 365 Tyr Sy Sy l Ie Val 445 Al a Phe Leu Thr Thr 525 Val G u 270 Al a Asp SIle Val Val 350 Pr o Asn Thr Sy Tyr 430 Leu Lys G u G n G u 510 Pr o G u Asp Gy 255 Ile u Asp Tyr Val Pro Arg Lys 320 Thr Asp 335 Thr Thr Al a Al a Ser Leu Phe Al a 400 Tyr Thr 415 Asp Al a G y G y G y Thr Arg Ile 480 Phe Pro 495 Thr Phe Lys Thr Phe AsD <210> 557 <211> 874 <212> DNA <213> Corynebacteri um gl utami cum <220> Page 714 Gene Sequence 24-2-06.txt <221> CDS <222> (101)..(874) <223> RXN02213 <400> 557 ttctgtggaa tgagaatccg atgtttttct cacgccggct gcgaaatctc accctaaaaa agt t agaatt ggagctcact aac Asn gac Asp t cc Ser aac Asn t cc Ser cag G n gag G u aac Asn ggc G y aac Asn 150 aac Asn at t lie cca Pr o ct g Leu ttc Phe ttc Phe aag Lys acc Thr cca Pr o ttc Phe gtt Val gcc Al a 120 cca Pr o gag G u cgc Ar g t ac Tyr acc Thr 200 at c SIle gct Al a ct c Leu ct c Leu gag G u at c lile ggt Sy gca Al a at g et gca Al a t ac Tyr gtt Val 170 gct Al a at c SIle ggc Gy aag Lys tct Ser gga Gy cac Hi s gaa G u 75 gt c Val ct c Leu gt c Val ct g Leu cag G n 155 cct Pr o cgc Ar g ggt Gy tgg Tr p agc Ser gca Al a gag G u at t lle at c SIle cct Pr o ggt G y at t SIle gct Al a 140 ttc Phe cca Pr o gt c Val acc Thr ggc G0y 220 ctt Leu cct Pr o ctt Leu gct Al a ttc Phe gt a Val gac Asp 110 cac Hi s aac Asn cgt Ar g acc Thr ttc Phe 190 t cc Ser ggt Gy gaa G u 15 ggc Gy ctt Leu at c l Ie acc Thr gtt Val 95 cct Pr o t cc Ser gtt Val tgg Tr p ggt Gy 175 gac Asp cac Hi s ggc Gy gtt Val at g bet cgt Ar g gcc Al a cca Pr o 80 gac Asp aac Asn gt c Val gag G u ggt Gy 160 at c SIle aac Asn acc Thr at t SIle cagccgaagc agacgccgtc gtg act gaa agc aag 11! Val Thr G u Ser Lys 1 ggc gac aag tcc tat 16 G y Asp Lys Ser Tyr gag aag ctg ccg tac 21 G u Lys Leu Pro Tyr acc gaa gac ggc gca Thr G u Asp G y Al a aac tgg gat gca tct Asn Trp Asp Ala Ser gcc cgt gtt ctc atg Ala Arg Val Leu IVet ctc gca acc atg cgt Leu Ala Thr IVet Arg 100 gac gtc aac cca ctg Asp Val Asn Pro Leu 115 atc gtg gag gct ttc 49! Ile Val G u Ala Phe 130 atc gag tac gag cgc 54 ie G u Tyr u Arg 145 tcc gag tcc ttc tcc 59! Ser G u Ser Phe Ser 165 gtc cac cag gtc aac 64; Val His G n Val Asn 180 gag ggc ctt gca tac 69 G u G y Leu Al a Tyr 195 acc atg gaa aac ggc 73! Thr IVet u Asn y 210 gag gct gaa gca gca 78 G u Ala G u Ala Ala 995 atg ctc ggc cag cca gtg tcc atg ctg atc cct cgc gtt gtt ggc ttc Page 715 Gene Sequence 24-2-06.txt Met Leu G y G n Pro Val Ser Met Leu Ile Pro Arg Val Val G y Phe 230 235 240 245 aag ttg acc ggc gag atc cca gta ggc gtt acc gca act Lys Leu Thr Gy G u Ile Pro Val Gy Val Thr Ala Thr 250 255 <210> 558 <211> 258 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 558 Val Thr G u Ser 1 G y Asp Lys Ser G u Lys Leu Pro Thr G u Asp G y Asn Trp Asp Ala Ala Arg Val Leu Leu Al a Thr IVet 100 Asp Val Asn Pro 115 Ile Val u Ala 130 Ile G u Tyr G u 145 Ser G u Ser Phe Val His G n Val 180 G u G y Leu Al a 195 Thr IVet G u Asn 210 G u Al a G u Al a 225 Arg Val Val y Al a Thr Lys 5 Tyr Tyr Al a Ser vet Ar g Leu Phe Ar g Ser 165 Asn Tyr Gy Al a Phe 245 Asn Ser Phe Asn Ala 10 Asp Tyr Phe Ala Leu Ser Leu Lys Val Leu 40 Asn Ile Thr Asn G u 55 Ser Asp Pro Ser lie 70 G n Asp Phe Thr G y 90 G u Ala Val Ala Ala 105 Asn Pro Ala G u Ibet 120 G y Arg Pro Asp Al a 135 Asn G u G u Arg Tyr 150 Asn Phe Arg Val Val 170 Ile u Tyr Leu Ala 185 Pro Asp Thr Cys Ile 200 Leu G y Ile Leu G y 215 IVet Leu G y G n Pro 230 Lys Leu Thr G y G u 250 Lys Ser Gy Hi s G u Val Leu Val Leu G n 155 Pr o Ar g Sy Tr p Val 235 I le Ser Al a G u SIle I le Pr o Sy SIle Al a 140 Phe Pr o Val Thr Sy 220 Ser Pr o Thr Val Asn G u G n Cys Sy Asp 125 Lys Leu Sy Val Asp 205 Val bet Val G u Sy Leu SIe Thr Val Pr o Ser Val Tr p Sy 175 Asp Hi s Gy I e Val 255 <210> 559 <211> 817 Page 716 Gene Sequence 24-2-06.txt <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(817) <223> FRXA02213 <400> 559 ttctgtggaa tgagaatccg atgtttttct cacgccggct cagccgaagc agacgccgtc gcgaaatctc accctaaaaa agt t agaatt ggagctcact aac Asn gac Asp t cc Ser aac Asn t cc Ser cag G n gag G u aac Asn ggc Gy aac Asn 150 aac Asn at t SIle cca Pr o ttc Phe ttc Phe aag Lys acc Thr cca Pr o ttc Phe gtt Val gcc Al a 120 cca Pr o gag Gu cgc Ar g t ac Tyr acc Thr 200 gct Al a ct c Leu ct c Leu gag Su at c SIle ggt Sy gca Al a at g Met gca Al a t ac Tyr gtt Val 170 gct Al a at c SIle agc Ser gca Al a gag Gu at t I e at c SIle cct Pr o ggt G y at t SIle gct Al a 140 ttc Phe cca Pr o gt c Val acc Thr ctt Leu cct Pr o ctt Leu gct Al a ttc Phe gt a Val gac Asp 110 cac Hi s aac Asn cgt Ar g acc Thr ttc Phe 190 t cc Ser gaa Gu 15 ggc Gy ctt Leu at c l Ie acc Thr gtt Val 95 cct Pr o t cc Ser gtt Val tgg Tr p ggt Gy 175 gac Asp cac Hi s gtt Val at g Net cgt Ar g gcc Al a cca Pr o 80 gac Asp aac Asn gt c Val gag Gu ggt Gy 160 at c SIle aac Asn acc Thr gtg act Val Thr 1 ggc gac Sy Asp gag aag G u Lys acc gaa Thr G u aac tgg Asn Trp gcc cgt Al a Ar g ctc gca Leu Al a gac gtc Asp Val atc gt g Ile Val 130 atc gag Ile G u 145 t cc gag Ser G u gtc cac Val Hi s gag ggc Gu Gy acc at g Thr lbet 210 aag Lys ct g Leu gac Asp gat Asp gtt Val acc Thr aac Asn 115 gag G u t ac Tyr t cc Ser cag G n ctt Leu 195 gaa G u t cc Ser ccg Pr o ggc Gy gca Al a ct c Leu at g et 100 cca Pr o gct Al a gag G u ttc Phe gt c Val 180 gca Al a aac Asn gaa agc aag G u Ser Lys t at Tyr t ac Tyr gca Al a tct Ser at g let cgt Ar g ctg Leu ttc Phe cgc Ar g t cc Ser 165 aac Asn t ac Tyr ggc Gy ctg ggc atc ctg ggc tgg ggc gtt ggt ggc att gag gct gaa gca gca Page 717 Gene Sequence 24-2-06.txt Leu Gy Ile Leu Gy Trp Gy Val Gy Gy Ile Gu Ala Gu Ala Ala 215 220 225 at g ct c ggc cag cca gtg tcc atg ctg at c Mbt Leu G y G n Pro Val Ser Mbt Leu Ile 230 235 <210> 560 <211> 239 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 560 Val Thr G u Ser 1 G y Asp Lys Ser G u Lys Leu Pro Thr G u Asp G y Asn Trp Asp Ala Ala Arg Val Leu Leu Al a Thr IVet 100 Asp Val Asn Pro 115 Ile Val u Ala 130 Ile G u Tyr G u 145 Ser G u Ser Phe Val His G n Val 180 G u G y Leu Al a 195 Thr IVet G u Asn 210 G u Al a G u Al a 225 <210> 561 <211> 2891 <212> DNA Lys 5 Tyr Tyr Al a Ser vet Ar g Leu Phe Ar g Ser 165 Asn Tyr Gy Al a Asn Ser Phe Asn Ala 10 Asp Tyr Phe Ala Leu Ser Leu Lys Val Leu 40 Asn Ile Thr Asn G u 55 Ser Asp Pro Ser lie 70 G n Asp Phe Thr G y 90 G u Ala Val Ala Ala 105 Asn Pro Ala G u Ibet 120 G y Arg Pro Asp Al a 135 Asn G u G u Arg Tyr 150 Asn Phe Arg Val Val 170 Ile u Tyr Leu Ala 185 Pro Asp Thr Cys Ile 200 Leu G y Ile Leu G y 215 IVet Leu G y G n Pro 230 Lys Ser Gy Hi s G u Val Leu Val Leu G n 155 Pr o Ar g Sy Tr p Val 235 Ser Al a G u SIle I le Pr o Sy SIle Al a 140 Phe Pr o Val Thr Sy 220 Ser Thr Val Asn G u G n Cys Sy Asp 125 Lys Leu Sy Val Asp 205 Val bet G u Sy Leu SIe Thr Val Pr o Ser Val Tr p Sy 175 Asp Hi s Gy lie <213> <220> <221> <222> Cor ynebact eri um gl ut arri cum CDS (1)..(2868) Page 718 Gene Sequence 24-2-06.txt <223> RXA02056 <400> 561 cgc Ar g 1 gt c Val cgc Ar g t ac Tyr acc Thr gac Asp cca Pr o gca Al a tgg Tr p gag Su 145 ggt Sy cgc Ar g at c SIle gga G y ct g Leu 225 ggc G y tct Ser t cc Ser gca Al a cac Hi s tct Ser at g Net acc Thr cgc Ar g 115 cag G n cac Hi s ttc Phe cgc Ar g gac Asp 195 cca Pr o gcc Al a aag Lys cca Pr o 5 gat Asp ct c Leu gt g Val ct g Leu gtt Val gtt Val cgt Ar g ggc G y ct g Leu ggc G y 165 gcg Al a gac Asp cca Pr o gag G u ttc Phe 245 cgt Ar g tac Ty r ggc G y at c SIle ct c Leu 70 cct Pr o gat Asp gga G y at g bet acc Thr 150 aag Lys tac Ty r gag G u acc Thr gct Al a 230 t cc Ser ct a Leu cca Pr o gtt Val cag G n acc Thr t ac Tyr aag Lys cac Hi s cca Pr o 135 at c SIle gag G u acc Thr cgc Ar g cag G n 215 ttc Phe ct c Leu acc Thr gca Al a ggc G y 40 ggt G y gat Asp acc Thr aac Asn ct c Leu 120 gtt Val tgg Tr p acc Thr ct c Leu acc Thr 200 gca Al a gag gau gaa G u aag Lys gag G u aaa Lys gct Al a gat Asp cca Pr o acc Thr 105 at c SIle cca Pr o gat Asp at g let aag Lys 185 tgg Tr p gag G u aac Asn ggt G y ggc G y 10 ttc Phe ctt Leu gt g Val t cc Ser at g Net 90 cgc Ar g gct Al a gac Asp ct g Leu acc Thr 170 gt c Val ct g Leu cag G n ttc Phe gca Al a 250 cag G n cag G n gt c Val t cc Ser ttc Phe 75 cgt Ar g gt c Val gac Asp cac Hi s gac Asp 155 ct g Leu ggc G y cag G n aag Lys ct g Leu 235 gaa G u ggc G y ggc G y acc Thr ggt G y tgg Tr p tgg Tr p at g let acc Thr cgc Ar g 140 cgt Ar g cgc Ar g t cc Ser gac Asp t ac Tyr 220 cag G n gca Al a acc Thr gct Al a at c SIle gaa G u gat Asp gca Al a cag G n aac Asn 125 gac Asp acc Thr gag G u gaa G u cgc Ar g 205 at c SIle acc Thr ctt Leu at c SIle t ca Ser acc Thr ttc Phe gag G u cag G n ct c Leu 110 cca Pr o ct c Leu ttc Phe gt a Val t ac Tyr 190 ct c Leu ct g Leu aag Lys at c SIle at c I e gaa G u t cc Ser ct g Leu at c SIle gac Asp at t SIle ctt Leu gac Asp aac Asn ct g Leu 175 acc Thr gag G u cag G n t ac Tyr cca Pr o 255 ggt G y gac Asp acc Thr cgc Ar g ttc Phe gtt Val gag G u t ca Ser at c SIe gtc Val 160 t cc Ser cac Hi s gcc Al a aag Lys gtc Val 240 ctg Leu atg gac tcc gcc atc gac acc gcc gca ggc caa ggc ctc gac gaa gtt Page 719 lIet Asp Ser Ala lie Asp Thr 260 Gene Sequence 24-2-06.txt Al a Al a G y n G y Leu \sp G u Val 270 gt c Val gt g Val gag G u 305 t cc Ser t cc Ser gaa G u ggc G y ggc G y 385 t ac Tyr ttc Phe t ac Tyr cca Pr o cgc Ar g 465 ggc G y gag G u ct g Leu ggt Sy 275 aag Lys ggc G y ggc G y act Thr at t SIle 355 act Thr ggc G y gt c Val acc Thr aag Lys 435 gca Al a ggc G y aac Asn at c SIle gga 15y 515 cca Pr o ct g Leu at c SIle cac Hi s 325 aac Asn cgc Ar g gt g Val gt g Val ggc G y 405 cca Pr o ttc Phe gt c Val gac Asp gct Al a 485 ggc gay gga G y cac Hi s gca Al a ggt G y 310 ct g Leu ccg Pr o gca Al a cca Pr o cca Pr o 390 acc Thr gac Asp ggc G y tgg Tr p gt c Val 470 gat Asp cgc Ar g gac Asp cgt Ar g t cc Ser 295 ggc G y cag G n t cc Ser aag Lys ct g Leu 375 gaa G u at c SIle t cc Ser tgc Cys gtt Val 455 ttc Phe gat Asp gag G u ct c Leu 265 cgc Ar g ttc Phe ggt G y ttc Phe ct g Leu 345 gac Asp ct c Leu at c SIle at c SIle cgc Ar g 425 gt c Val cag G n gac Asp t cc Ser gtt Val 505 aac Asn 2 ct c Leu aac Asn gac Asp ggc G y 330 gaa G u t ac Tyr cac Hi s aac Asn gt g Val 410 t cc Ser ttc Phe ct g Leu ct c Leu at g Net 490 cgt Ar g gaa G u aac Asn gag G u gt g Val 315 gac Asp gct Al a ct g Leu ggt G y ct g Leu 395 gt g Val at g Met cac Hi s gcc Al a gt c Val 475 acc Thr gct Al a gat Asp gt g Val ttt Phe 300 aag Lys ggc G y gtt Val gac Asp gac Asp 380 gct Al a aac Asn cac Hi s gt c Val acc Thr 460 tgc Cys cag G n cag G n gca Al a ct g Leu 285 gaa G u t ac Tyr gag G u aac Asn aag Lys 365 gct Al a aag Lys aac Asn t ac Tyr aac Asn 445 gag G u t ac Tyr cca Pr o t ac Tyr gaa G5u 525 ttc Phe ggc G y cac Hi s at c SIle cca Pr o 350 ggc G y gca Al a ct g Leu cag G n gca Al a 430 ggc G y t ac Tyr cgc Ar g aag Lys acc Thr 510 gca Al a aac Asn caa G n ct c Leu aag Lys 335 gcg Al a gt a Val ttc Phe cgt Ar g at c SIe 415 acc Thr gac Asp cgt Ar g ct c Leu at g bet 495 gaa G u gt c Val 864 912 960 1008 1056 1104 1152 1200 1248 1296 1344 1392 1440 1488 1536 1584 1632 cgc gac ttc cac gac cag atg gaa tct gtg ttc aac gaa gtc aag gaa Page 720 Gene Sequence 24-2-06.txt Su Ser Val Phe Asn G u Val Arg Asp 530 ggc ggc Gy y 545 aag ctt Lys Leu gaa ct g G u Leu cca cgt Pro Ar g ggt ggc Gy Gy 610 gct aac Al a Asn 625 ggt acc G y Thr gaa gag Gu Gu ggt aag G y Lys ggc ttc G y Phe 690 tgg gaa Trp u 705 gat gag Asp u ct g at c Leu II e tct tcc Ser Ser at g act let Thr 770 cgt cgt Arg Arg 785 Phe His Asp G n Met 535 Lys G u aag Lys cca Pr o gga G y gt g Val 595 at c SIle t cc Ser ttc Phe ttc Phe ttc Phe 675 gag G u gca Al a t ac Tyr ctt Leu gca Al a 755 gtt Val cac Hi s cag G n ggc G y 565 gct Al a ccc Pr o tgg Tr p cgc Ar g cag G n 645 cca Pr o gt c Val G y ttc Phe t cc Ser 725 ct g Leu at c l Ie cag G n ct g Leu gct Al a 550 ctt Leu tt c Phe gtt Val gca Al a tt g Leu 630 cgc Ar g ct c Leu tac Tyr tac Tyr ggc G y 710 t ca Ser cct Pr o gag G u cca Pr o t cc Ser 790 gag G u gag G u gcc Al a gct Al a tgg Tr p 615 gtt Val cac Hi s cac Hi s aac Asn t cc Ser 695 gac Asp ggc G y cac Hi s cgc Ar g t cc Ser 775 gac Asp gca Al a acc Thr aac Asn aag Lys 600 ggc G y cgc Ar g gca Al a gag G u t cc Ser 680 gt a Val ttc Phe gaa G u ggc G y ttc Phe 760 acc Thr ct g Leu cag G n aac Asn acc Thr 585 aag Lys gag G u ctt Leu gtt Val ctt Leu 665 gca Al a gga G y gcc Al a gct Al a t ac Tyr 745 ct g Leu cca Pr o aag Lys acc Thr at c SIle 570 cca Pr o cgc Ar g ct c Leu gca Al a gcc Al a 650 gca Al a ct g Leu aac Asn aac Asn aag Lys 730 gaa G u cag G n gca Al a cgt Ar g ggc G y 555 t cc Ser gaa G u gt c Val ct c Leu ggt G y 635 at c l Ie cag G n acc Thr gaa G u ggc G y 715 tgg Tr p ggc G y ct g Leu aac Asn cca Pr o 795 540 at c SIle cgt Ar g ggc G y t cc Ser gcc Al a 620 gaa G u gac Asp t cc Ser gag G u gac Asp 700 gct Al a ggc G y cag G n tgc Cys cac Hi s 780 ct g Leu acc Thr gaa G u ttc Phe tct Ser 605 ttc Phe gat Asp cca Pr o aag Lys t ac Tyr 685 t cc Ser cag G n cag G n ggc G y gct Al a 765 ttc Phe gtt Val ggc G y gag G u aac Asn 590 gt c Val ggt G y t cc Ser gcg Al a ggc G y 670 gca Al a at c SIle acc Thr acc Thr cca Pr o 750 gag G u cac Hi s at c SIle t cc Ser ct c Leu 575 t ac Tyr acc Thr t cc Ser cgc Ar g acc Thr 655 aac Asn ggc G y gtt Val at c SIe t cc Ser 735 gac Asp ggt G y ct a Leu ttc Phe 1680 1728 1776 1824 1872 1920 1968 2016 2064 2112 2160 2208 2256 2304 2352 2400 2448 ccg aag tcc atg ctg cgt aac aag gct gct gcc tcc gca cca gaa gac Page 721 Pro Lys Ser IVet Leu Arg 805 Gene Sequence 24-2-06.txt Asn Lys Ala Ala Ala Ser Ala Pro u Asp 810 815 ttc Phe gca Al a t ac Tyr gcg Al a 865 t cc Ser cag G n ct c Leu cgc Ar g gag G u 945 gag G u gca Al a 835 gaa G u gtt Val gct Al a gag G u gag G u 915 cag G n aag Lys acc aag Thr Lys aag gt g Lys Val gca aag Al a Lys atc gaa Ile G u 870 gcc ggc Al a G y 885 gca aac Al a Asn atc ccg Ile Pro tcc acc Ser Thr ctt atc Leu II e 950 ttc Phe aag Lys cgc Ar g 855 at g Net t ac Tyr cag G n aac Asn gca Al a 935 t cc Ser 825 gt c Val gag G u cac Hi s aac Asn cca Pr o 905 cca Pr o ggt G y gt g Val at g Net aag Lys cca Pr o gct Al a 890 tgg Tr p aag Lys gtt Val at c SIle ct g Leu gac Asp at t SIle 875 gag G u ccg Pr o at g bet gcc Al a aac Asn gt c Val gga Gy 860 ccg Pr o gaa G u ttc Phe cgc Ar g aag Lys 940 gat Asp t cc Ser 845 cgc Ar g ttc Phe gt c Val t ac Tyr cgc Ar g 925 gt g Val cca Pr o 830 ggc Gy gac Asp aac Asn ct c Leu cag G n 910 gtt Val cac Hi s aac Asn aag Lys gac Asp cgc Ar g ttc Phe 895 gag G u t cc Ser cag G n 2496 2544 2592 2640 2688 2736 2784 2832 2878 2891 gac gag gct ttc Asp G u Al a Phe gag gct G u Al a 955 t aagt ct t t a t agt cct gca ct a <210> 562 <211> 956 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 562 Arg His Ser Val Pro Arg Leu Thr Lys 1 5 Val y Ser IVet Asp Tyr Pro Ala G u Arg Leu Ala G u Leu G y Val y Lys 40 Tyr Asp His Arg Val I e n y Al a Thr IVet Ser Arg Leu Leu Thr Asp Asp 70 Asp Ala IVet Asn Val Pro Tyr Thr Pro Pro Asn Thr G y Val Asp Lys Asn Thr 100 105 G y G n i 10 Phe G n Leu Val Val Ser Ser Phe IVet Arg 90 Arg Val I Page 722 Thr Al a SIle G u Asp Al a G n I e G u Ser Leu SIe Asp I e Gene Sequence 24-2-06.txt Al a Tr p G u 145 Sy Ar g SIle Sy Leu 225 Gy Vet Val Val G u 305 Ser Ser G u Sy Sy 385 Tyr Phe Tyr Pr o Ar g 115 G n Hi s Phe Ar g Asp 195 Pr o Al a Lys Ser Sy 275 Lys Sy Sy Thr SIle 355 Thr Sy Val Thr Lys 435 Al a Ser Arg y His Leu lie Ala Asp Thr Asn Pro Leu Ser 125 Sy Leu Sy 165 Al a Asp Pr o G u Phe 245 I le Pr o Leu SIle Hi s 325 Asn Ar g Val Val Sy 405 Pr o Phe Val Pr o 135 I le G u Thr Ar g G n 215 Phe Leu Thr Ar g Ser 295 Sy G n Ser Lys Leu 375 G u SIle Ser Cys Val 455 120 Val Tr p Thr Leu Thr 200 Al a G u G u Al a Sy 280 I le Ser let Hi s G n 360 Leu Thr Hi s Ser Pr o 440 Sy Pr o Asp let Lys 185 Tr p G u Asn Gy Al a 265 Ar g Phe Sy Phe Leu 345 Asp Leu SIle SIle Ar g 425 Val G n Asp Leu Thr 170 Val Leu G n Phe Al a 250 Sy Leu Asn Asp Sy 330 G u Tyr Hi s Asn Val 410 Ser Phe Leu Hi s Asp 155 Leu Sy G n Lys Leu 235 G u G n Asn G u Val 315 Asp Al a Leu Sy Leu 395 Val let Hi s Al a Ar g 140 Ar g Ar g Ser Asp Tyr 220 G n Al a Sy Val Phe 300 Lys Sy Val Asp Asp 380 Al a Asn Hi s Val Thr 460 Asp Thr G u G u Ar g 205 I le Thr Leu Leu Leu 285 G u Tyr G u Asn Lys 365 Al a Lys Asn Tyr Asn 445 G u Leu Phe Val Tyr 190 Leu Leu Lys SIle Asp 270 Phe Sy Hi s SIle Pr o 350 Sy Al a Leu G n Al a 430 Sy Ty r Asp Asn Leu 175 Thr G u G n Tyr Pr o 255 G u Asn G n Leu Lys 335 Al a Val Phe Ar g SIe 415 Thr Asp Ar g Arg Phe y Lys Asp Val Phe Ile Asp Leu Val Cys Tyr Arg Leu Arg Page 723 Gene Sequence 24-2-06.txt 465 Gy G u Leu Ar g Gy 545 Lys G u Pr o Gy Al a 625 Gy G u Gy Gy Tr p 705 Asp Leu Ser let Ar g 785 Pr o Phe 475 Thr G n Pro Lys Asn SIle Gy 515 Phe Lys Pr o Gy Val 595 I le Ser Phe Phe Phe 675 G u Al a Tyr Leu Al a 755 Val Hi s Ser G u Al a 485 Gy Sy Asp G n Sy 565 Al a Pr o Tr p Ar g G n 645 Pr o Val Sy Phe Ser 725 Leu l Ie G n Leu Leu 805 Thr Asp Ar g Asp G n Al a 550 Leu Phe Val Al a Leu 630 Ar g Leu Ty r Ty r Gy 710 Ser Pr o G u Pr o Ser 790 Ar g Lys Asp Pro Ser G u Thr Val 505 Leu Ser Asn 520 [et G u Ser 535 G u Al a G n G u Thr Asn Al a Asn Thr 585 Ala Lys Lys 600 Trp G y u 615 Val Arg Leu Hi s Al a Val Hi s G u Leu 665 Asn Ser Al a 680 Ser Val G y 695 Asp Phe Al a G y G u Al a Hi s G y Tyr 745 Arg Phe Leu 760 Ser Thr Pro 775 Asp Leu Lys Asn Lys Al a Phe G n Ser 825 Met 490 Ar g G u Val Thr SIle 570 Pr o Ar g Leu Al a Al a 650 Al a Leu Asn Asn Lys 730 G u G n Al a Ar g Al a 810 Val Al a Asp Phe Sy 555 Ser G u Val Leu Sy 635 l le G n Thr G u Sy 715 Tr p Sy Leu Asn Pr o 795 Al a SIle G n Tyr Thr 510 Ala G u Al a 525 Asn G u Val 540 I le Thr G y Arg G u G u G y Phe Asn 590 Ser Ser Val 605 Al a Phe G y 620 G u Asp Ser Asp Pro Al a Ser Lys G y 670 G u Tyr Al a 685 Asp Ser Ile 700 Al a G n Thr G y G n Thr Sn G y Pr o 750 Cys Ala G u 765 Hi s Phe Hi s 780 Leu Val I e Ser Ala Pro Asn Asp Pro 830 Net 495 G u Val Lys Ser Leu 575 Tyr Thr Ser Ar g Thr 655 Asn Sy Val SIle Ser 735 Asp Sy Leu Phe G u 815 Asn Tyr Asp Val G u G n 560 Leu Hi s G u Leu Ar g 640 Al a Asn let Al a SIe 720 Lys Hi s Ser Leu Thr 800 Asp Val Page 724 Ala Asp Ala Ala Lys Val Lys 835 Tyr Tyr G u Leu Ala Lys Arg 850 855 Ala lie Val Arg Ile u IVet 865 870 Ser G u Al a Leu Al a G y Tyr 885 G n Asp G u Pro Ala Asn n 900 Leu Pro G u Leu lie Pro Asn 915 Arg Ala G n Ser Ser Thr Ala 930 935 G u G u Lys G n Leu I e Asp 945 950 <210> 563 <211> 1517 <212> DNA <213> Cor ynebact er i um gl ut ar <220> <221> CDS <222> (1)..(1494) <223> RXA01745 Gene Sequence 24-2-06.txt Lys Val et Leu Val Ser G y 840 845 Lys G u Lys Asp G y Arg Asp 860 Leu His Pro lie Pro Phe Asn 875 Pro Asn Al a G u G u Val Leu 890 Sy Pro Trp Pro Phe Tyr G n 905 910 IVet Pro Lys IVet Arg Arg Val 920 925 Thr G y Val Ala Lys Val Hi s 940 G u Ala Phe G u Ala 955 Lys Asp Ar g Phe 895 G u Ser G n at c I e gaa G u aag Lys gt g Val cag G n ctt Leu gca Al a gt c Val <400> 563 atc ctt gca I e Leu Al a 1 cgc atc ggt Arg lie G y gct cct gcc Al a Pro Al a gca gct cct Al a Al a Pr o atg cca gaa IVet Pro G u ct c aag gct Leu Lys Al a gtc tcc acc Val Ser Thr acc at c gt g Thr Ile Val 115 gaa G u 5 gca Al a gag G u gct Al a ggc Sy ggc Sy aag Lys at c SIle gac Asp aac Asn gaa G u cca Pr o gaa G u 70 gac Asp gt c Val ctt Leu gac Asp gca Al a gaa G u gca Al a 55 t cc Ser acc Thr gac Asp gca Al a acc Thr gct Al a cca Pr o 40 gca Al a gt c Val gt c Val acc Thr gac Asp 120 gt c Val gca Al a gtt Val act Thr acc Thr gaa G u gaa G u 105 gaa G u gac Asp 10 gca Al a aag Lys ggc G y gaa G u gt a Val 90 at c SIle gac Asp gt c Val cct Pr o gaa G u gcc Al a ggc G y 75 gac Asp cca Pr o gac Asp ggc Sy gcc Al a gag G u gca Al a acc Thr gaa G u t cc Ser acc Thr gca Al a gaa G u cca Pr o acc Thr at t SIle cca Pr o cca Pr o gt c Val 125 gt c Val gag G u aag Lys gat Asp acc Thr ctt Leu gt a Val 110 gac Asp gcc Al a gca Al a gag G u gaa G u tgg Tr p gag gcu ggc G y ggc G y Page 725 Gene Sequence 24-2-06.txt gca Al a gaa G u 145 cca Pr o act Thr gtt Val gtt Val aag Lys 225 gct Al a aag Lys at c SIle acc Thr aag Lys 305 ct g Leu aac Asn t cc Ser acc Thr aag Lys 385 at c l Ie gaa G u aag Lys cct Pr o t ac Tyr 195 ttg Leu gat Asp gct Al a aag Lys gcg Al a 275 ct g Leu aag Lys ttc Phe aac Asn gtt Val 355 gt c Val at t SIle cgc Ar g gct Al a gag G u 165 gcg Al a acc Thr acc Thr ttg Leu gtt Val 245 cgt Ar g aag Lys gag G u gcg Al a gt g Val 325 tct Ser ct c Leu cac Hi s gac Asp atc ggt gac gca aac gca gct gca gca cct gcc Asn Ala Ala Ala Ala Pro Ala SI e cct Pr o 150 ccc Pr o gca Al a cca Pr o gt g Val gct Al a 230 t cc Ser ggt Gy acc Thr gt c Val tt c Phe 310 aag Lys tt c Phe t cc Ser gat Asp ct g Leu 390 u y 135 gcc Al a aag Lys t cc Ser ct g Leu acc Thr 215 gct Al a gct Al a acc Thr gt c Val gat Asp 295 at c SIle gca Al a aac Asn at c SIle gct Al a 375 gct Al a Asp Al a gaa G u aag Lys gca Al a gt g Val 200 ggt Gy gcg Al a tgg Tr p act Thr gag G u 280 at g let gag G u gtt Val gcg Al a gct Al a 360 cag G n gat Asp gag G u gaa Gu act Thr 185 cgc Ar g acc Thr aac Asn t cc Ser cag G n 265 gct Al a act Thr aag Lys gt c Val aag Lys 345 gtt Val gat Asp cgt Ar g gag G u gag G u 170 gt g Val aag Lys ggt Gy ggc Gy act Thr 250 aag Lys ct g Leu cgc Ar g cac Hi s gag G u 330 acc Thr gat Asp ct c Leu t ca Ser gaa G u 155 ccc Pr o t cc Ser ctt Leu at c SIle gag Gu 235 aag Lys gt c Val cag G n gtt Val ggt Gy 315 gct Al a aag Lys acc Thr t cc Ser cgc Ar g 395 cca Pr o aag Lys gct Al a gct Al a ggt Gy 220 gct Al a tct Ser aac Asn at t SIle gct Al a 300 gt g Val ttg Leu gag G u cca Pr o at c SIle 380 aac Asn gtt Val aag Lys tct Ser gaa G u 205 ggc Gy gca Al a gtt Val cgc Ar g tct Ser 285 gag G u aac Asn gtt Val at g Net gct Al a 365 cca Pr o aac Asn aag Lys gaa G u ggc Gy 190 aag Lys cgt Ar g cct Pr o gac Asp at c SIle 270 gct Al a ct g Leu ct c Leu t cc Ser acc Thr 350 ggt Gy gag G u aag Lys gaa G u gca Al a 175 gac Asp cac Hi s at c SIe gct Al a cct Pr o 255 cgt Ar g cag G n cgt Ar g act Thr cat Hi s 335 t ac Tyr ct g Leu at c SIe ct g Leu 432 480 528 576 624 672 720 768 816 864 912 960 1008 1056 1104 1152 1200 Page 726 cca Pr o gaa G u at c SIle gat Asp acc Thr 465 acc Thr cag Gn Gene Sequence 24- 2- 06. txt gat ctg tcc ggt ggc acc ttc acc atc acc aac att Asp Leu Ser G y y Thr Phe Thr Ile Thr Asn Ile 405 410 gca ctg tct gat acc cca atc ctg gtt cca cca cag Ala Leu Ser Asp Thr Pro Ile Leu Val Pro Pro G n 420 425 430 ggc acc ggc gcg atc gtg aag cgt cca gtt gtc atc Sy Thr Gy Al a lie Val Lys Arg Pro Val Val lie 435 440 445 att gat tcc atc gcg atc cgt cag atg gtc ttc cta Ile Asp Ser Ile Ala lle Arg n IVet Val Phe Leu 455 460 gac cac cag gtt gta gat ggc gca gat gct ggt cgc Asp His G n Val Val Asp G y Ala Asp Ala G y Arg 470 475 atc aag gac cgc ctt gag acc gct aac ttc gaa ggc Ile Lys Asp Arg Leu G u Thr Ala Asn Phe G u y 485 490 taagatctct gcaagttaaa acc ggt Gy 415 gct Al a acc Thr cca Pr o ttc Phe gat Asp 495 1248 1296 1344 1392 1440 1488 1517 <210> 564 <211> 498 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 564 I e 1 Ar g Al a Al a et Leu Val Thr Al a Gu 145 Pr o Leu Al a Asp I le G y Asp Pro Al a G u Ala Pro G u Pro G u Leu Lys Al a Val Ser Thr Asp 100 Ile Val u 115 Val Ile Al a 130 Su u Al a Lys Lys G u G u 5 Al a G u Al a Gy Gy Lys SIle Ar g Al a G u 165 Asp Asp Thr Al a G u Al a 55 Ser Thr Asp Al a Gy 135 Al a Lys Al a Pr o 40 Al a Val Val Thr Asp 120 Asp Gu Lys Val Asp 10 Al a Al a Val Lys Thr G y Thr G u G u Val 90 G u Ile 105 Su Asp Al a Asn G u Gu G u G u 170 Val Pr o G u Al a Gy 75 Asp Pr o Asp Al a Gu 155 Gy Al a G u Al a Thr G u Ser Thr Al a 140 Pr o Al a G u Pr o Thr SIle Pr o Pr o Val 125 Al a Val I e G u Lys Val G n Leu Al a Val Pr o Gu Al a Al a G u G u Tr p G u Gy Gy Al a Gu 160 Pro Lys Lys u Ala Ala 175 Page 727 Thr Thr Pro Ala Ala Ala Ser 180 Gene Sequence 24- 2- 06. txt Ala Thr Val Ser Ala Ser y 185 190 Asp Asn Val Val Lys 225 Al a Lys SIle Thr Lys 305 Leu Asn Ser Thr Lys 385 Pr o G u SIle Asp Thr 465 Thr G n Tyr 195 Leu Asp Al a Lys Al a 275 Leu Lys Phe Asn Val 355 Val SIle Asp Al a Gy 435 I le Asp Thr Thr Leu Val 245 Ar g Lys G u Al a Val 325 Ser Leu Hi s Asp Ser 405 Ser Gy Ser G n Leu Thr 215 Al a Al a Thr Val Asp 295 l le Al a Asn SIle Al a 375 Al a Gy Thr SIle Al a 455 Val Val 200 Gy Al a Tr p Thr G u 280 let G u Val Al a Al a 360 G n Asp Thr Pr o Val 440 l le Asp Ar g Thr Asn Ser G n 265 Al a Thr Lys Val Lys 345 Val Asp Ar g Phe SIle 425 Lys Ar g Gy Lys Gy Gy Thr 250 Lys Leu Ar g Hi s G u 330 Thr Asp Leu Ser Thr 410 Leu Ar g G n Al a Al a 490 Leu SIle G u 235 Lys Val G n Val Sy 315 Al a Lys Thr Ser Ar g 395 I le Val Pr o let Asp 475 Al a Sy 220 Al a Ser Asn SIle Al a 300 Val Leu G u Pr o SIle 380 Asn Thr Pr o Val Val 460 Al a G u 205 Sy Al a Val Ar g Ser 285 G u Asn Val let Al a 365 Pr o Asn Asn Pr o Val 445 Phe Sy Hi s SIle Al a Pr o 255 Ar g G n Ar g Thr Hi s 335 Tyr Leu SIle Leu Sy 415 Al a Thr Pr o Phe Sy Ar g G u 240 G u G u Leu Lys Tyr 320 Pr o Hi s Leu Al a Lys 400 Ser Sy G u Leu Leu 480 Thr lie Lys Asp 485 Leu Arg Leu G u Thr Asn Phe G u G y Asp Leu 495 <210> 565 <211> 1005 <212> DNA <213> Corynebacter i um gl ut ai cum Page 728 Gene Sequence 24-2-06.txt <220> <221> CDS <222> (101)..(982) <223> RXA00782 <400> 565 gggtatggat gcagcggctg atcacgctgc ccatttggcc gttcgcaacc gctaattagt taaggagcac ctgtttaatc aatcttgccc agcacggcca atg tct att ttt ctc Met Ser lie Phe Leu aat t ca Asn Ser t ca gag Ser G u ggc acc G y Thr gag ttg G u Leu gcg gat Al a Asp atc att Ile Ile gag ggc Gu Gy aag gt g Lys Val act ccc Thr Pro 135 t cc ggc Ser G y 150 atg at g rvet et ggc ggt G y G y ttt gaa Phe G u ggt gga G y G y 215 gat Asp cat Hi s aac Asn cct Pr o gt c Val gaa G u at c SIle gga G y 120 ggc G y ccg Pr o t ac Tyr gac Asp gct Al a 200 cgc Ar g cgt Ar g cgc Ar g ttt Phe gt a Val at c SIle gt g Val acc Thr tct Ser ggg G y ctt Leu 170 at c SIle cct Pr o at c SIle cga Ar g aaa Lys ggc G y at t I e 75 gac Asp cgt Ar g cgc Ar g ctt Leu tt g Leu 155 t ca Ser at c SIle gag G u at c SIle at t SIle gct Al a act Thr 60 ttc Phe gct Al a gac Asp at c SIle gcg Al a 140 at c SIle gat Asp ggt G y acc Thr cgc Ar g 220 at t SIle tta Leu ggg G y 45 gtt Val gtt Val cac Hi s gct Al a at t SIle 125 gga G y t ca Ser at t SIle aca Thr aag Lys 205 cag G n gcc Al a caa G n aag Lys cct Pr o at c SIle tct Ser 110 ggc G y at t SIle aag Lys ggc G y acc Thr 190 gca Al a ggc G y 15 tct Ser acc Thr gaa G u cca Pr o cca Pr o 95 gag G u cct Pr o acg Thr tcg Ser at t SIle 175 cat Hi s at c l Ie at t SIle ggt G y at t SIle gca Al a gcc Al a 80 ct g Leu gcg Al a aac Asn ccg Pr o gga G y 160 tct Ser at c SIle gt c Val 1 acc Thr gcg Al a ttg Leu at g Met ttt Phe tgc Cys tgg Tr p tgc Cys gca Al a 145 aca Thr acg Thr gac Asp at g bet ggt G y aag Lys at c SIle gag G u gcc Al a gt g Val gct Al a cca Pr o 130 aac Asn ct g Leu gcg Al a gct Al a at c SI e 210 tcg Ser ct c Leu aat Asn gaa G u aaa Lys at t SIle t at Tyr 115 ggc G y at t SIle act Thr at t l Ie ct g Leu 195 ggt G y gaa G u gt g Val gac Asp acg Thr gct Al a at t SIle 100 gcc Al a at t SIle gca Al a t at Tyr ggt G y 180 gag G u gag G u ggt G y ggt G y act Thr ggt G y gcg Al a act Thr aag Lys att SIe ggt G y cag G n 165 att SIe gcc Al a at c SIe gat gca gag gaa Asp Al a (3 u u gct gct gac ttc att tct aag cac gtg Ala Ala Asp Phe IIe Ser Lys His Val 225 Page 729 ac a Thr 230 aag Ly s gcg Al a ac a Thr tt g Le u ene Sequence 24- 2- 06. t xt aaa cca gtt gt g ggt tac gt g gca ggc t tt acc gcc cct gaa gga Lys Pr o Val Val G31y Tyr Val Al a G31y Phe Thr Al a Pro O31u G31y 235 240 245 acc at g ggg cat gct ggc gcc at c gt g aca ggt t ca gaa ggc act Thr I'b t G31y Hi s Al a (1y Al a Ilie Val Thr 31y Ser O31u 1y Thr 250 255 260 cga gca aag aag cat gca t tg gag gcc gt g ggt gt t cgc gt g gga Ar g Al a Lys Lys Hi s Al a Leu O31u Al a Val G31y Val Ar g Val G31y 265 270 275 act ccg agt gaa acc gcg aag ct t at g cgt gag gt a gt t gca gct Thr Pr o Ser O31u Thr Al a Lys Leu I'b t Ar g O31u Val Val Al a Al a 280 285 290 t aact aacag gccacagat c tt a 835 883 931 979 1 005 <210O> 566 <21 1> 294 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ari cumn <400> 566 I'bt Ser I Ie Phe Leu Asn Ser Asp Ser Thr Al a Le u vb t Ph e cys Tr p cys Al a 145 Thr Thr As p Ser Le u As n (31u Lys5 I Ie Tyr 115 G31y I Ie Thr I Ie Le u 1 95 G31y G31y Thr G31y Al a Thr Lys5 I Ie G31y O31n 1 65 li1e Al a (31u Thr Le u As p I Ie G31y ValI Pr o 135 G31y vb t G31y (31u Hi s As n 40 Pr o ValI (31u I Ie G31y 120 G31y Pr o Tyr As p Al a 200 Al a Pr a ValI Thr Al a Pr a 1 05 Hi s Ou le Ou Pr a 1 85 As p I Ie Ar g Lys5 G31y I Ie As p Ar g Ar g Le u Le u 1 Ser I Ie (31u le le Al a Thr Ph e Al a As p I Ie Al a 140 li1e As p G31y Thr I Ie Le u G31y ValI ValI Hi s Al a I Ie 125 G31y Ser I Ie Thr Lys5 205 O31n Al a O31n Lys5 Pr a I Ie Ser 110 G31y I Ie Ly s G31y Thr 1 Al a G31y Ser Thr (31u Pr a Pr a (31u Pr a Thr Se r I Ie 1 Hi s I Ie I'bt Ile Oy Ou Ile Oy OyAsp AlaOu OuArg AaAaAsp Phe Page 730 ene Sequence 24-2-C 210 215 220 li1e Ser Lys Hi s Val Thr Lys Pr o Val Val G31y Ty r 225 230 235 Thr Ala Pr o 31u G31y Lys Thr N/t G31y Hi s Ala G31y 245 250 G31y Ser O31u G31y Thr Ala Ar g Ala Lys Lys Hi s Ala 260 265 G31y Val Ar g Val G31y Thr Thr Pr o Ser O31u Thr Ala 275 280 (31u Val Val Ala Ala Leu 290 <210> 567 <211> 1395 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> ODS <222> (1372) <223> RXA00783 <400> 567 aaagt tccca aggggt gggg gct gagcacc acggat ccaa tt t t t tacagt gt t agact tcac aat acgat ca t at tggt gag tt g Le u t xt Val Ala G31y Phe 240 Ala I Ie Val Thr 255 Leu 03 u A a Val 270 Lys Leu l'b t Ar g 285 t gt tgca at t tgcaaag aaa cac t ta ct t 11! Lys Hi s Leu Leu t ta Le u g aa (31u tt t Ph e cca Pr o gt c ValI cgt Ar g ct c Le u c ag (31n g aa G31u g aa G31u ac c Thr gcg A a gct A a gca A a at g vb t gct A a 120 tt t Ph e gca A a ggt G31y ag g Ar g gt c ValI ac g Thr at c I Ie at t I Ie gt t ValI gt g ValI gt g ValI aaa Ly s aag Ly s 75 t cg Ser aaa Ly s gct A a aaa Ly s g at As p cca Pr o gcg A a gt g ValI gct A a gga G31y gag (31u g ac As p ct t Le u gt g ValI 45 gct A a ggc G31y c ag O31n c ac Hi s g aa O31u 125 gca A a tt t Ph e tt g Le u gag O31u gga G31y gct A a act Thr 110 t ac Tyr g aa O31u 15 g aa O31u aag Lys5 g aa O31u cgt Ar g tt t Ph e 95 gt t ValI t at Tyr ggc G31y t ac Tyr gga G31y at c G31y g at As p aat As n tt c Ph e aag Lys5 gca A a gt g ValI gga G31y gcg A a gcg A a gt g ValI at t I Ie 130 ggg G31y g ac As p t ca Ser ac c Thr ggc G31y gcg A a 1 00 gt g ValI tt g Le u cgc gcg aat cgt t cg t at ct g gct at g t gc t ct gt t gaa ggt ggc at g Page 731 Arg Ala Asn Arg 135 Ser Tyr Leu Gene Sequence 24-2-06.txt Ala let Cys Ser Val G u Gy Gy bet gag G u 150 gaa G u gt c Val gt g Val gtt Val ctt Leu 230 aac Asn ct g Leu gt g Val at c SIle ttc Phe 310 ct c Leu gt g Val gga Gy cgc Ar g t at Tyr 390 gag Su gat Asp gct Al a at c SIle 200 gt g Val ggc Gy ggt Sy gcc Al a at c SIle 280 gct Al a gac Asp gt g Val ggt Gy ttg Leu 360 gat Asp cac Hi s ct g Leu ct c Leu 170 ggc Gy at c SIle ccg Pr o at c SIle ttg Leu 250 aag Lys ggc G y gct Al a ggt Sy ctt Leu 330 at c SIle gt g Val aac Asn ttg Leu gcg Al a 155 act Thr ttt Phe tgg Tr p tt g Leu acg Thr 235 gct Al a aat Asn aat Asn ggt G y ggc G y 315 ggg Gy acc Thr ct c Leu aac Asn gt c Val 395 140 aag Lys ggt Gy gaa G u cag G n gt g Val 220 ct g Leu gaa G u gat Asp ggt G y gaa G u 300 gga G y gat Asp gcg Al a ggc Gy gt g Val 380 acc Thr gaa G u at t SIle act Thr gt g Val 205 ct c Leu gat Asp tct Ser ct g Leu gca Al a 285 cgc Ar g gca Al a agc Ser tgt Cys gat Asp 365 gt g Val gtt Val aag Lys gat Asp gag G u 190 t at Tyr acg Thr gat Asp gcc Al a aac Asn 270 ggt G y cat Hi s t ca Ser cag G n gat Asp 350 caa G n gaa G u gt g Val cct Pr o gag G u 175 gt g Val t ac Tyr gat Asp aac Asn ggt G y 255 t ac Tyr ttg Leu ggt G y gct Al a gt a Val 335 gt g Val gca Al a ggc ggy gag G u gaa G u 160 gac Asp gca Al a gaa G u gac Asp gct Al a 240 ggc G y gt g Val gt g Val ggg G y gaa G u 320 cgc Ar g gt g Val acg Thr aga Ar g ggt G0y 400 145 gct Al a aaa Lys gag G u gag G u ggc Gy 225 gat Asp ttg Leu aaa Lys at g et cag G n 305 tcg Ser agt Ser gca Al a aag Lys cga Ar g 385 at g let ttg Leu gcg Al a aaa Lys gaa G u 210 gat Asp ttc Phe gac Asp ctt Leu t cc Ser 290 cgc Ar g at g let gt g Val aag Lys cct Pr o 370 at c SIle gat Asp gca Al a cgg Ar g gt c Val 195 gca Al a gt g Val cgc Ar g at t SIle gat Asp 275 acg Thr ccc Pr o gct Al a ttt Phe gga G y 355 ctt Leu ct c Leu gca Al a aag Lys gag G u 180 at t SIe aca Thr at t SIe cat Hi s ttg Leu 260 ggc G y ttg Leu gcg Al a gct Al a gt g Val 340 at c SIe gt g Val gcg Al a gcg Al a 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 gat cac gct gcc cat ttg gcc aat ctt gcc cag cac ggc cag ttc gca Page 732 Gene Sequence 24-2-06.txt Asp His Ala Ala His Leu Ala Asn Leu Ala G n His G y Gn Phe Ala 410 415 420 acc gct aat tagttaagga gcacctgttt aat Thr Al a Asn <210> 568 <211> 424 <212> PRT <213> Cor ynebact eri um gl ut am cum 1395 Leu 1 Ser G n SIle Gy Lys Al a G n Ser Val 145 Al a Lys G u G u Gy 225 Asp Leu Lys 0> 568 Lys Hi s Lys Hi s Al a Ar g Val Ala G y Leu Al a G y Al a Asp Val vbet 115 II e Leu 130 G u G y Leu Al a Al a Ar g Lys Val 195 G u Al a 210 Asp Val Phe Arg Asp Il e Leu Asp 275 Leu Leu 5 G y Pro Asp Leu Ser Thr Thr Val G y Val Ala I e 100 Val Ala Leu Asp G y let Lys Val 165 G u Ile 180 lie Pro Thr Leu I e Al a His Asp 245 Leu G u 260 G y Ser Ar g let G u G u 55 Lys Val Gy Gy Al a 135 I le Val Thr Leu G u 215 Asp Ar g Lys Gy G u G u Thr 40 Al a Al a Al a vet Al a 120 Asn G u Asp Al a SIle 200 Val Gy Gy Al a SIle 280 Asp Leu 25 Hi s Al a G n Pr o Asp 105 Asp Ar g SIle Pr o Al a 185 Lys Asn Lys Al a Lys 265 I le Phe 10 Al a Gy Ar g Val Thr 90 I e I e Ser Leu Leu 170 Gy I e Pr o SIle Leu 250 Lys Val Val Val Lys Lys 75 Ser Lys Al a Tyr Al a 155 Thr Phe Tr p Leu Thr 235 Al a Asn Lys Asp Pr o Al a Val Al a Gy G u Leu 140 Lys Gy G u G n Val 220 Leu G u Asp Asp Leu Val Al a Gy G n Hi s G u 125 Al a Su I e Thr Val 205 Leu Asp Ser Leu Al a Phe Leu G u Gy Al a Thr 110 Tyr let Lys Asp G u 190 Tyr Thr Asp Al a Asn 270 G u G u Lys G u Ar g Phe Val Tyr Cys Pr o G u 175 Val Tyr Asp Asn Gy 255 Tyr Gy Tyr Gy SIe Gy Asp Asn Phe Ser G u 160 Asp Al a G u Asp Al a 240 Gy Val Sy Asn y Ala y Leu Val 285 Page 733 Met G n 305 Ser Ser Al a Lys Ar g 385 Met Hi s Thr Pr o Al a Phe Sy 355 Leu Leu Al a G n Asp Asn Gy 325 Asn Val Val G u Al a 405 Al a Val 295 Leu Asp Phe Al a Leu 375 Asn Hi s Al a Gene Sequence 24-2-06.txt Ala Ala Ala Gy u Arg His 300 Asp ie G y G y G y Al a Ser 315 Val lie Leu y Asp Ser G n 330 G y G y Ile Thr Al a Cys Asp 345 350 Leu Asp Val Leu y Asp G n 360 365 Asp y Asn Asn Val Val G u 380 His Pro Leu Val Thr Val Val 395 Ala Ala His Leu Ala Asn Leu 410 Asn Gy Al a Val 335 Val Al a Gy G u Al a 415 <210> 569 <211> 1623 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1600) <223> RXN01695 <400> 569 tatggccaac acttgcattc gggtgctggc gat tgttcggcag agaactcgcg gagataaaag gaa aag Lys gcc Al a cca Pr o gag G u tgc Cys aag Lys gca Al a at c lile tgg Tr p t cc Ser ct g Leu gt a Val agg Ar g agc Ser cag G n ccg Pr o t ac Tyr at c I e acc Thr acg Thr gt c Val aac Asn cca Pr o gag G u gag G u ggt G y gag G u gca Al a gtt Val ttc Phe catttat gagatgacgc cttgtgttgg igttgaac atg tca gat tcc ccg IVet Ser Asp Ser Pro 1 gca gat gta gtt ctc att ggt Ala Asp Val Val Leu Ile y 15 gca atg ctg cgt cag ctg gag Ala IVet Leu Arg G n Leu G u cgt ttg gat gga ccg gca caa Arg Leu Asp y Pro Ala G n gga acc ggc cac tct gct cta G y Thr G y His Ser Ala Leu aag ggc aag gtt gaa att gcc Lys y Lys Val G u lie Ala 80 cag gtt tcc cgt cag ttc tgg G n Val Ser Arg G n Phe Trp 95 100 Page 734 Gene Sequence 24-2-06.txt tct Ser aac Asn t ac Tyr ggc G y 150 ttg Leu at c SIle ct g Leu gt c Val aac Asn 230 gt c Val cca Pr o cgt Ar g ggc G y acc Thr 310 ggc G y aag Lys cag G n ct c Leu gtt Val 120 aag Lys acc Thr gca Al a gaa G u gca Al a 200 agc Ser cac Hi s gca Al a gt c Val acc Thr 280 gca Al a gtt Val acc Thr at c SIle ttt Phe 360 gaa G u cac Hi s cgc Ar g gct Al a ggc G y 170 acc Thr gaa G u aag Lys ggc Gy gga Gy 250 ggc Gy gag G u gtt Val gag G u aag Lys 330 cca Pr o ct g Leu gag G u gt a Val tac Ty r gac Asp 155 cgt Ar g gac Asp gtt Val gct Al a gac Asp 235 tac Ty r tt c Phe gaa G u ggc Sy ggt G y 315 tt c Phe gac Asp acc Thr gga gtg ctg tct G y Val Leu Ser 110 tct ttc ggc cag Ser Phe G y n 125 gaa gct ttg aag G u Ala Leu Lys 140 gat gaa gct acc Asp u Al a Thr gac ttc tct gat Asp Phe Ser Asp 175 atc aac tac ggt Ile Asn Tyr G y 190 gaa ggc act gaa G u G y Thr G u 205 gat ggc gca aag Asp G y Al a Lys 220 acc aag acc atc Thr Lys Thr Ile gca ctg gat ctg Ala Leu Asp Leu 255 gct gga ttc cca Ala y Phe Pro 270 ctg atc gag cag Leu lie G u G n 285 gct cct cca at g Ala Pro Pro IVet 300 gaa aag ggt ctg G u Lys G y Leu ttg aag gaa ggc Leu Lys G u G y 335 aac att cct tcc Asn lie Pro Ser 350 aag tac ctt gtc Lys Tyr Leu Val 365 gat Asp ggc G y gat Asp ttc Phe 160 cca Pr o gct Al a at c SIle tgg Tr p aag Lys 240 ctt Leu gt a Val cac Hi s tct Ser ct c Leu 320 t cc Ser t ac Tyr act Thr cct aag gaa Pro Lys G u 115 gca gat cag Al a Asp G n 130 cac cca ct c His Pro Leu 145 acc gag aag Thr G u Lys gt a gca at c Val Ala lle cag acc aag G n Thr Lys 195 cgc t at ggc Arg Tyr G y 210 at c gt g acc Ile Val Thr 225 gca aac ttc Al a Asn Phe cgc agc gca Arg Ser Ala t cc ggc ct g Ser G y Leu 275 gca gcc aag Al a Al a Lys 290 gtt cct cac Val Pro Hi s 305 ttt gga cct Phe G y Pr o t ac ct g gac Tyr Leu Asp ctt ggc gt t Leu G y Val 355 gaa gtt ct c G u Val Leu 370 ttc atc Phe I e gtt Val ttc Phe ct g Leu tct Ser 180 cag G n cac Hi s gt c Val gt g Val ggc Gy 260 tgg Tr p gt a Val ctt Leu t ac Tyr ct g Leu 340 gct Al a aag Lys gca Al a cag G n cct Pr o 165 tgg Tr p t ac Tyr gaa G u aag Lys ttc Phe 245 at c SIe ctt Leu t at Tyr gac Asp ggt G y 325 ttc Phe gct Al a gac Asp 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 Page 735 Gene Sequence 24-2-06.txt cag G n aac Asn 390 aag Lys ttg Leu cct Pr o tgc Cys at c SIle 470 cag G n aag cgt at g Lys Arg IVet gat tgg gag Asp Trp u gca gga ttc Al a G y Phe 410 aac aac tcc Asn Asn Ser 425 gca t cc at c Ala Ser Ile 440 ggt gac cgc G y Asp Ar g t cc tac ggc Ser Tyr G y tgg gca cgc Tr p Al a Ar g 490 gat gct ctt cgc gag Asp Ala Leu Arg G u 380 acc atc gtt gcc gga Thr lie Val Ala y 395 cct aag ttc ggt tcc Pro Lys Phe G y Ser 415 gaa ggc acc atc gcc Su G y Thr Ile Ala 430 gca cct tcc gca atg Ala Pro Ser Ala Mbet 445 atg atc gag tgg ggc IVet lie u Trp y 460 aag aag ctt gct tcc Lys Lys Leu Ala Ser 475 acc cag aag acc ctg Thr G n Lys Thr Leu 495 tac at g Tyr Mbet 385 cag cgt G n Arg 400 ct g aa Leu G u gga ttg G y Leu at c gag lie G u gac aag Asp Lys 465 gag cca G u Pro 480 aag ctt Lys Leu cca gag gca caa Pro u Ala G n gtt Val ttc Phe ct c Leu ct g Leu 450 ct g Leu gca Al a gag G u cag G n ggc G y ggt G y 435 ctt Leu aag Lys ct g Leu gaa G u gtt Val acc Thr 420 gct Al a gag G u gac Asp ttt Phe gcc Al a 500 1267 1315 1363 1411 1459 1507 1555 1600 1623 taaatcttct aactgctttc ttt <210> 570 <211> 500 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 570 IVet Ser Asp Ser Pro Lys Asn Ala Pro 1 5 Val Val Leu Ile Oy Ala Gy Ile bet Leu Arg G n Leu G u Pro Ser Trp Thr 40 Asp y Pro Ala G n u Ser Ser Ser G y Hi s Ser Al a Leu Cys G u Leu Asn 70 Lys Val G u I e Ala Lys Ala Val y Ser Arg G n Phe Trp Ser His Leu Val 100 105 Pro Lys G u Phe lie Asn Pro Val Pro 115 120 Ala Asp G n Val Ala Tyr I e Lys Ala 130 135 Arg Ile 10 Ser Ser G n lie Pro Trp Tyr Thr I Ile Asn 90 G u G u His Val Arg Tyr Page 736 Asp Leu Phe Asn G u Lys Val Phe 125 Al a Al a Al a Ar g Sy Lys G n Ser G n Lys Gene Sequence 24-2-06.txt His Pro Leu Phe G n 145 Thr Val G n Ar g SIle 225 Al a Ar g Ser Al a Val 305 Phe Tyr Leu G u vet 385 Ar g G u Leu G u Lys 465 Pr o Lys SIle Lys 195 Gy Thr Phe Al a Leu 275 Lys Hi s Pr o Asp Val 355 Leu G u G n Gy Gy 435 Leu Lys Leu Pr o 165 Tr p Tyr G u Lys Phe 245 l le Leu Tyr Asp Gy 325 Phe Al a Asp G n SIle 405 Thr Ser Ar g et G u 485 let let Asp Asp Lys 215 Val Gy G n Cys Lys 295 Ar g Tr p Ser G u Asp 375 Gy Pr o SIle Gy Phe 455 Pr o G n Thr Tyr Ala Asp 155 Asp G u Ala Thr Phe Al a G u Al a 200 Ser Hi s Al a Val Thr 280 Al a Val Thr SIle Phe 360 Lys Asp Al a Asn Al a 440 Sy Ser Tr p Lys Sy 185 Al a SIle Thr Sy Lys 265 Asn Ser SIle Pr o Ar g 345 Asp Ar g Tr p Sy Asn 425 Ser Asp Tyr Al a Ar g Asp Val Al a Asp 235 Tyr Phe G u Gy Gy 315 Phe Asp Thr Asp Thr 395 Pr o G u Al a et Lys 475 Thr Asp SIle G u Asp 220 Thr Al a Al a Leu Al a 300 G u Leu Asn Lys Al a 380 I le Lys Sy Pr o SI e 460 Lys G n Phe Asn Sy 205 Sy Lys Leu Sy SIle 285 Pr o Lys Lys SIle Tyr 365 Leu Val Phe Thr Ser 445 G u Leu Lys Ser Tyr 190 Thr Al a Thr Asp Phe 270 G u Pr o Gy Su Pr o 350 Leu Ar g Al a Gy I e 430 Al a Tr p Al a Thr Asp 175 Sy G u Lys SIe Leu 255 Pr o G n let Leu Sy 335 Ser Val G u Sy Ser 415 Al a Net Sy Ser Leu 495 Leu G u G u Al a Page 737 ene Sequence 24- 2- 06. txt 500 <210O> 571 <21 1> 1039 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1039) <223> FRXA01615 <400> 571 t at ggccaac act tgcat tc gggt gct ggc gat cat t tat tgttcggcag agaactcgcg gagataaaag gaagttgaac gagat gacgc ct tgt gt tgg at g t ca gat t cc ccg 11! l'bt Ser Asp Ser Pr o aag Ly s gcc A a cca Pr o gag O31u t gc cys aag Ly s t ct Ser aac As n t ac Ty r ggc G31y 1 50 t tg Le u at c I Ie gca A a at c I Ie t gg Tr p t cc Ser ct g Le u gt a ValI ct c Le u gt t ValI 120 aag Ly s ac c Th r gca A a g aa O31u ag g Ar g ag c Ser c ag O31n ccg Pr o t ac Ty r at c I Ie g aa O31u c ac Hi s cgc Ar g gct A a ggc G31y 1 70 ac c Th r ac c Th r ac g Th r gt c ValI aac As n cca Pr o gag O31u gga G31y t ct Ser g aa O31u 140 g at As p g ac As p at c I Ie g at As p ct g Le u tt c Ph e 45 aat As n gag O31u aag Ly s gt g ValI tt c Ph e 125 gct A a g aa O31u tt c Ph e aac As n gag O31u ggt (31y gag O31u gca A a gt t ValI tt c Ph e ct g Le u 110 ggc G31y t tg Le u gct A a t ct Ser t ac Tyr 1 90 gca A a 15 gca A a cgt Ar g gga O31y aag Ly s c ag O31n 95 t ct Ser c ag O31n aag Ly s ac c Thr g at As p 1 75 ggt G31y g at As p at g vb t tt g Le u ac c Thr ggc G31y gt t ValI g at As p ggc G31y g at As p tt c Ph e 1 cca Pr a gct A a 1 gt a ValI ct g Le u g at As p ggc G31y aag Ly s t cc Ser cct Pr a gca A a c ac Hi s 145 ac c Thr gt a ValI c ag O31n gt t ValI cgt Ar g gga O31y c ac Hi s gt t ValI cgt Ar g aag Ly s g at As p 130 cca Pr a gag O31u gca A a ac c Thr ct c Le u c ag G n ccg Pr a t ct Ser g aa O3u c ag O3n g aa O3u 115 c ag O3n ct c Le u aag Ly s at c I Ie aag Ly s 1 at t lIe ct g Le u gca A a gct A a at t I Ie tt c Ph e 1 00 tt c Ph e gt t ValI tt c Ph e ct g Le u t ct Se r 1 c ag O31n Page 738 Gene Seq ctg gat gca gct gaa gtt gaa ggc act Leu Asp Ala Ala G u Val G u Gy Thr 200 205 gtc aag agc atc aag gct gat ggc gca Val Lys Ser Ile Lys Al a Asp G y Al a 215 220 aac gta cac act ggc gac acc aag acc Asn Val His Thr G y Asp Thr Lys Thr 230 235 gtc ggc gca ggc gga tac gca ctg gat Val y Ala Gy y Tyr Ala Leu Asp 250 cca cag gtc aag ggc ttc gct gga ttc Pro G n Val Lys y Phe Ala y Phe 265 270 cgt tgc acc aac gag gaa ctg atc gag Arg Cys Thr Asn G u G u Leu Ile G u 280 285 ggc aag gca tct gtt ggc gct cct cca G y Lys Al a Ser Val G y Al a Pro Pro 295 300 acc cgc gtt atc Thr Arg Val lle 310 <210> 572 <211> 313 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 572 IVet Ser Asp Ser Pro Lys Asn Ala Pro 1 5 Val Val Leu Ile Gy Ala Gy Ile bet Leu Arg G n Leu G u Pro Ser Trp Thr 40 Asp y Pro Ala G n u Ser Ser Ser G y Hi s Ser Al a Leu Cys G u Leu Asn 70 Lys Val G u Ile Ala Lys Ala Val y Ser Arg G n Phe Trp Ser His Leu Val 100 105 Pro Lys G u Phe Ile Asn Pro Val Pro 115 120 Ala Asp G n Val Ala Tyr Ile Lys Ala 130 135 His Pro Leu Phe n G y Iet Thr Tyr 145 150 luence 24-2-06. t xt gaa atc cgc tat ggc Su lie Arg Tyr y 210 aag tgg atc gtg acc Lys Trp Ile Val Thr 225 atc aag gca aac ttc Ile Lys Ala Asn Phe 240 ctg ctt cgc agc gca Leu Leu Arg Ser Ala 255 cca gta tcc ggc ct g Pro Val Ser y Leu 275 cag cac gca gcc aag G n His Ala Ala Lys 290 atg tct gtt cct cac [et Ser Val Pro His 305 cac Hi s gt c Val gt g Val ggc Gy 260 tgg Tr p gt a Val ctt Leu 739 787 835 883 931 979 1027 1039 Arg Ile 10 Ser Ser G n lie Pro Trp Tyr Thr I Ile Asn 90 G u G u His Val Arg Tyr Al a Asp 155 Page 739 Asp Leu Phe Asn G u Lys Val Phe 125 Al a G u Al a Al a Ar g Gy Lys G n Ser G n Lys Thr Thr G u Lys Leu Pro Leu IVet 165 Val Ala lie Ser Trp Ile Asp 180 G n Thr Lys G n Tyr Leu Asp 195 Arg Tyr G y His G u Val Lys 210 215 lie Val Thr Val Lys Asn Val 225 230 Ala Asn Phe Val Phe Val y 245 Arg Ser Ala Gy lie Pro On 260 Ser y Leu Trp Leu Arg Cys 275 Al a Al a Lys Val Tyr G y Lys 290 295 Val Pro His Leu Asp Thr Arg 305 310 <210> 573 <211> 233 <212> DNA <213> Cor ynebact er i um gl ut a <220> <221> CDS <222> <223> FRXA01695 <400> 573 gcc gga ttg ctc ggt gct tcc Al a G y Leu Leu G y Al a Ser 1 5 atg atc gag ctg ctt gag cgt IVbt I e G u Leu Leu G u Ar g ggc gac aag ctg aag gac atg G y Asp Lys Leu Lys Asp IVet tcc gag cca gca ctg ttt gag Ser G u Pro Ala Leu Phe G u 55 ctg aag ctt gag gaa gcc taa< Leu Lys Leu G u G u Al a <210> 574 <211> <212> PRT <213> Cor ynebact er i um gl ut a Gene Sequence 24-2-06.txt Ala Lys y Arg Asp Phe Ser 170 G u G y Thr Asp I e Asn Tyr 185 190 Ala Ala u Val u Gy Thr 200 205 Ser lie Lys Ala Asp Gy Ala 220 His Thr G y Asp Thr Lys Thr 235 Al a G y G y Tyr Al a Leu Asp 250 Val Lys y Phe Ala y Phe 265 270 Thr Asn G u G u Leu Ile G u 280 285 Al a Ser Val G y Al a Pro Pro 300 Val lle Asp 175 Gy G u Lys SIe Leu 255 Pr o G n Mbt cct gga gca tcc atc gca cct tcc gca Pro Gy Ala Ser Ie Ala Pro Ser Ala 10 tgc ttc ggt gac cgc atg atc gag tgg Cys Phe y Asp Arg IVet lie u Trp 25 atc cct tcc tac ggc aag aag ctt gct lie Pro Ser Tyr y Lys Lys Leu Ala 40 cag cag tgg gca cgc acc cag aag acc Sn G n Trp Ala Arg Thr G n Lys Thr atcttct aactgctttc ttt Page 740 Gene Sequence 24 <400> 574 Ala Gy Leu Leu Gy Ala Ser Pro Gy Ala Ser 1 5 10 ebt Ile G u Leu Leu u Arg Cys Phe Gy Asp 25 Sy Asp Lys Leu Lys Asp IVet lie Pro Ser Tyr 40 Ser G u Pro Ala Leu Phe u G n n Trp Ala 55 Leu Lys Leu G u G u Al a <210> 575 <211> 1063 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1063) <223> RXA00290 <400> 575 agctacagat ttagctagtg tttttgttcc agaaccctaa gagcttcccg caaaaacacc gattaacaag gctaaatgat -2-06. txt lie Ala Pro Ser Ala Arg Iet Ile G u Trp G y Lys Lys Leu Ala Arg Thr G n Lys Thr atgaggttct acccttaaca atg acc atc gac ctg 11! IVet Thr Ile Asp Leu cag G n gag G u cgc Ar g gca Al a aac Asn gat Asp ct g Leu gtt Val t cc Ser gga G y ctt Leu aag Lys gt c Val gga G y agc Ser gat Asp 120 caa G n ct c Leu ctt Leu gat Asp gtt Val cag G n ttc Phe gac Asp aac Asn t cc Ser gct Al a cca Pr o at t SIle 75 gcc Al a gct Al a gct Al a ct c Leu at t SIle t ac Tyr gag G u t cc Ser t cc Ser ggc G y ttg Leu cat Hi s t cc Ser cct Pr o gcg Al a ggc G y ccc Pr o aag Lys 110 gag G u gaa G u cgt Ar g gtt Val acc Thr gct Al a 80 at g Net at c SIle at c SIle 1 at c SIle ccg Pr o gct Al a cac Hi s gtt Val gag G u cct Pr o gca Al a ttc Phe ct c Leu cag G n acg Thr ctt Leu ggc G y at c SIle gcc Al a 130 gca Al a gac Asp tgt Cys at t SIle ctt Leu gct Al a 100 ttg Leu gcg Al a cac Hi s at g bet gaa G u gga G y ggc G y cag G n gac Asp cct Pr o tct ttc ggt gct atc aac ttg gag gac atc tcc gct cct cgt tgc ttc Page 741 Ser Phe y Ala lie Asn Leu 135 140 Gene Sequence 24-2-06.txt Su Asp lie Ser Ala Pro Arg Cys Phe 145 gag gtg gag cgc cgc ctc atc gag cgt G u Val G u Arg Arg Leu lie G u Arg 150 155 gat gac cag cac ggc acc gct gtg gtt Asp Asp G n His G y Thr Ala Val Val 170 tcc ctg aag ctg ctg gat cgc aag atc Ser Leu Lys Leu Leu Asp Arg Lys lie 185 190 tcc ggc gca ggc gca gcg ggc gtt gca Ser y Ala y Ala Ala y Val Al a 200 205 gct gga gca acc gac atc gtg gtt ctt Ala y Ala Thr Asp I e Val Val Leu 215 220 gac agc cgt gag gat ctt tcc cca gtt Asp Ser Arg G u Asp Leu Ser Pro Val 230 235 acc aac cct cgt ggc atc agc ggt ggc Thr Asn Pro Arg y lie Ser y G y 250 gcg gac ctg ttc att ggc gtg tcc ggc Ala Asp Leu Phe lie Gy Val Ser G y 265 270 ctc aaa ctc atg gcc ccg gag cca atc Leu Lys Leu IVet Ala Pro G u Pro lie 280 285 acc cca gag atc gat cct gag ct g t ct Thr Pro G u lie Asp Pro G u Leu Ser 295 300 gcg acc ggg ccg gtc ttg acc tgc cta Ala Thr Gy Pro Val Leu Thr Cys Leu 310 315 <210> 576 <211> 321 <212> PRT <213> Corynebacter i um gl ut am cum <400> 576 IVet Thr Ile Asp Leu G n Arg Ser Thr 1 5 IIe Phe G u Ala His G u G y G y Lys 25 Pro Leu Arg Asp IVet Arg Asp Leu Ser 40 Ala n Val Cys Gu Ala Ile Lys u 55 His Thr G y IIe G y Asn Thr Val Al a 70 ct c Leu at c SIle 175 gaa G u gct Al a gat Asp aag Lys at c SIle 255 ggc G y ct g Leu cag G n acc Thr gat Asp 160 ct c Leu gac Asp gt a Val t cc Ser gct Al a 240 aat Asn aac Asn ttc Phe aag Lys aga Ar g 320 at t SIle gct Al a ct c Leu gat Asp cga Ar g 225 gct Al a gag G u at c SIle acc Thr t ac Tyr 305 t ca Ser cca Pr o gcg Al a aag Lys at g bet 210 ggc Gy ctt Leu gct Al a ggc G y ct g Leu 290 ggc G y gtt Val ct g Leu at t SIle 195 ct g Leu at c SIle gca Al a ttc Phe gag G u 275 gcg Al a gcc Al a at g et cgc Ar g 180 gtt Val acc Thr at c SIle gag G u acc Thr 260 gac Asp aac Asn at c l Ie cac Hi s 165 aac Asn at t SIe aac Asn cac Hi s aag Lys 245 ggc G y gct Al a cca Pr o gt c Val 595 643 691 739 787 835 883 931 979 1027 1063 On Asn Leu Thr His Ou u 10 Leu Ser lie Ser Ser Thr Arg Leu Ala Tyr Thr Pro y Val Asp Pro G u Val Ala Arg Thr Val lie Ser Asp G y Thr Ala 75 Page 742 Gene Sequence 24-2-06.txt Val Leu G y Leu G u Pr o Al a Al a 145 I le Al a Leu Asp Ar g 225 Al a Su I e Thr Tyr 305 Lys Val 115 l le Ar g Val Leu SIle 195 Leu SIle Al a Phe G u 275 Al a Gy G n Asp Pr o Phe Hi s 165 Asn SIle Asn Hi s Lys 245 Gy Al a Pr o Asp lie Gy Pro n Ala Ser Leu Pro Val IVet Leu Val Ser G u 150 Asp Ser Ser Al a Asp 230 Thr Al a Leu Thr Phe Hi s Phe 135 Val Asp Leu Gy Gy 215 Ser Asn Asp Lys Pr o 295 Ser Asp 120 Gy G u G n Lys Al a 200 Al a Ar g Pr o Leu Leu 280 G u Ser 105 Val Al a Ar g Hi s Leu 185 Gy Thr G u Ar g Phe 265 let SIle Phe Asp l Ie Ar g Gy 170 Leu Al a Asp Asp Gy 250 I le Al a Asp Al a Al a Asn Leu 155 Thr Asp Al a SIle Leu 235 I le Gy Pr o Pr o Leu Val 125 G u G u Val Lys Val 205 Val Pr o Gy Ser Pr o 285 Leu Al a Thr SIe Leu SIe 175 G u Al a Asp Lys SIe 255 Gy Leu G n Gy Ala I e Val Al a Thr y Pro Val Leu Thr Cys Leu Thr 310 315 Ser <210> 577 <211> 1347 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1324) <223> RXN01048 <400> 577 agctacagat ttagctagtg tttttgttcc agaaccctaa atgaggttct acccttaaca gagcttcccg caaaaacacc gattaacaag gctaaatgat atg acc atc gac ctg 115 IVet Thr lie Asp Leu 1 cag cgt tcc acc caa aac ctc acc cat gag gaa atc ttc gag gca cac 163 Page 743 G n Ar g Ser Thr G n Asn Leu Gene Sequence 24- 2- 06. t xt Thr His Gu Gu Ile Phe Gu Ala His 15 gag G u cgc Ar g gca Al a aac Asn gat Asp ct g Leu gtt Val tct Ser gag G u 150 gat Asp t cc Ser t cc Ser gct Al a gac Asp 230 acc Thr gcg Al a gga G y ctt Leu aag Lys gt c Val gga Gy agc Ser gat Asp 120 ggt Sy gag Su cag G n aag Lys gca Al a 200 gca Al a cgt Ar g cct Pr o ct g Leu ct c Leu ctt Leu gat Asp gtt Val cag G n ttc Phe gac Asp at c SIle cgc Ar g ggc G y 170 ct g Leu gca Al a gac Asp gat Asp ggc Gy 250 at t SIle t cc Ser gct Al a cca Pr o at t SIle 75 gcc Al a gct Al a gct Al a aac Asn ct c Leu 155 acc Thr gat Asp gcg Al a at c SIle ctt Leu 235 at c SIle ggc Gy at t SIle t ac Tyr gag G u t cc Ser t cc Ser ggc Gy ttg Leu ttg Leu 140 at c SIle gct Al a cgc Ar g ggc Gy gt g Val 220 t cc Ser agc Ser gt g Val t cc Ser cct Pr o gcg Al a ggc Gy ccc Pr o aag Lys 110 gag G u gac Asp cgt Ar g gtt Val at c SIle 190 gca Al a ctt Leu gtt Val ggc Gy ggc G0y 270 act Thr ggt Gy cgc Ar g acc Thr gt c Val 95 gct Al a acc Thr at c SIle ct c Leu at c SIle 175 gaa G u gct Al a gat Asp aag Lys at c SIle 255 ggc Gy cgt Ar g gtt Val acc Thr gct Al a at g Net at c l Ie at c SIle t cc Ser gat Asp 160 ct c Leu gac Asp gt a Val t cc Ser gct Al a 240 aat Asn aac Asn ccg Pr o gct Al a cac Hi s gtt Val gag G u cct Pr o gca Al a gct Al a 145 at t SIle gct Al a ct c Leu gat Asp cga Ar g 225 gct Al a gag G u at c SIle ct c Leu cag G n acg Thr ctt Leu ggc Gy at c SIle gcc Al a 130 cct Pr o cca Pr o gcg Al a aag Lys at g Net 210 ggc Gy ctt Leu gct Al a ggc Gy gac Asp tgt Cys att SIe ctt Leu gct Al a 100 ttg Leu gcg Al a tgc Cys at g bet cgc Ar g 180 gtt Val acc Thr at c SIe gag G u acc Thr 260 gac Asp ctc aaa ctc atg gcc ccg gag cca atc ctg ttc acc ctg gcg aac cca Page 744 Leu Lys Leu IVet Ala Pro G u 280 Gene Sequence 24- 2- 06. t xt Pro lie Leu Phe Thr Leu Ala Asn Pro 285 290 acc Thr gcg Al a 310 ttc Phe acc Thr ct g Leu gcg Al a aaa Lys 390 cca Pr o gag G u ggg Gy gga Gy gag G u acc Thr 360 ccc Pr o ctt Leu ttt Phe gat Asp tct Ser ttc Phe 330 aag Lys agg Ar g cag G n aat Asn gag G u 300 ct g Leu ggc Gy gcc Al a gcc Al a agg Ar g 380 ctt Leu ct g Leu cct Pr o gct Al a gct Al a gca Al a 365 cag G n at c SIle tct Ser aac Asn ct c Leu cag G n 350 tcg Ser ct g Leu gac Asp aag Lys at c SIle 320 gcc Al a caa G n ct a Leu agg Ar g t cc Ser 400 ggc G y aac Asn gct Al a cag G n ccc Pr o 370 tcg Ser ccc Pr o gcc Al a gt g Val aag Lys aca Thr 355 tgg Tr p cca Pr o gt c Val at c l Ie ct g Leu aag Lys 340 tcg Ser at c SIle aag Lys gag G u gt c Val gcg Al a 325 at c SIe cag G n ccc Pr o cgc Ar g gcg Al a 1027 1075 1123 1171 1219 1267 1315 1347 taagagcaaa cttgaggccc aca <210> 578 <211> 408 <212> PRT <213> Corynebacter i um gl utam cum <400> 578 IVet Thr Ile Asp Leu G n Arg Ser Thr 1 5 IIe Phe G u Ala His G u G y G y Lys Pro Leu Arg Asp IVet Arg Asp Leu Ser 40 Ala n Val Cys Gu Ala lle Lys u His Thr G y IIe G y Asn Thr Val Ala 70 Val Leu y Leu y Asp lie y Pro G u G y Lys Al a G n Leu Phe Ser Ser 100 105 Pro lie Val Leu Asp Val His Asp Val 115 120 Al a Al a I e Al a Pro Ser Phe G y Al a 130 135 Sn Asn I 10 Leu Ser Leu Al a Asp Pro Val lie G n Al a 90 Phe Al a Asp Al a I I e Asn I Page 745 Thr Ser Thr Val Asp Leu Leu Val 125 G u G u Thr Sy Ar g Thr Val Al a Thr SIe Gene Sequence 24-2-06.txt Al a 145 I le Al a Leu Asp Ar g 225 Al a Su I e Thr Tyr 305 Asn Lys Ser Pr o Pr o 385 Leu Ar g Val Leu SIle 195 Leu SIle Al a Phe G u 275 Al a Al a Val Lys Thr 355 Tr p Pr o Val Cys Phe G u Val G u Arg Arg Leu lie G u Arg Leu Hi s 165 Asn SIle Asn Hi s Lys 245 Gy Al a Pr o Val Al a 325 I le G n Pr o Ar g Al a 405 150 Asp Ser Ser Al a Asp 230 Thr Al a Leu Thr Al a 310 Phe Thr Leu Al a Lys 390 Pr o Asp Leu Gy Gy 215 Ser Asn Asp Lys Pr o 295 Thr Pr o Pr o Ar g Leu 375 Thr SIle G n Lys Al a 200 Al a Ar g Pr o Leu Leu 280 G u Gy Gy G u Thr 360 Pr o Leu Phe Hi s Leu 185 Gy Thr G u Ar g Phe 265 Met SIle Ar g SIle et 345 Ser G n Lys Gy 170 Leu Al a Asp Asp Gy 250 I le Al a Asp Ser Phe 330 Lys Ar g G n Asn 155 Thr Asp Al a SIle Leu 235 I le Gy Pr o Pr o Asp 315 Al a Leu Ser Ser Leu 395 Al a Ar g Gy Val 220 Ser Ser Val G u G u 300 Leu Gy Al a Al a Ar g 380 Leu Val Lys Val 205 Val Pr o Gy Ser Pr o 285 Leu Pr o Al a Al a Al a 365 G n lie SIe 175 G u Al a Asp Lys SIe 255 Sy Leu G n G n Al a 335 Ar g Cys Ser Al a <210> 579 <211> 311 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> FRXA01048 <400> 579 cgc tct gac ttg cct aac cag atc aac aac gtg ctg gcg ttc cca gga Arg Ser Asp Leu Pro Asn n Ile Asn Asn Val Leu Ala Phe Pro y 1 5 10 Page 746 Gene Seq att ttc gcc ggc gct ctc gca gcc aag I e Phe Al a G y Al a Leu Al a Al a Lys 25 atg aag ctc gcc gct cag agg caa tcg Met Lys Leu Ala Ala G n Arg G n Ser 40 tcg agg tcg gcc gca tcg tgc cta ccg Ser Arg Ser Ala Ala Ser Cys Leu Pro 55 caa cag tca agg cag ctg tcc agg ccg Sn G n Ser Arg G n Leu Ser Arg Pro 70 aaa aat ttg ctt atc gac gcc tcc ctc Lys Asn Leu Leu Ile Asp Ala Ser Leu taagagcaaa cttgaggccc aca <210> 580 <211> 96 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 580 Arg Ser Asp Leu Pro Asn n Ile Asn 1 5 Ile Phe Al a y Ala Leu Ala Ala Lys 25 IVet Lys Leu Ala Ala G n Arg G n Ser 40 Ser Arg Ser Ala Ala Ser Cys Leu Pro Sn G n Ser Arg G n Leu Ser Arg Pro 70 Lys Asn Leu Leu Ile Asp Ala Ser Leu luence 24-2-06. t xt gct aag aag atc acc Ala Lys Lys Ile Thr cag aca tcg cag ctg G n Thr Ser G n Leu ccc tgg atc ccc gcg Pro Trp Ile Pro Ala tcg cca aag cgc aaa Ser Pro Lys Arg Lys ccc gtc gag gcg cca Pro Val G u Ala Pro 90 ccc Pr o agg Ar g ttg Leu acg Thr at a SIe Asn Al a G n Pr o Ser Pr o Val Lys Thr Tr p Pr o Val Al a SIle G n Pr o Ar g Al a Phe Thr Leu Al a Lys Pr o <210> 581 <211> 1063 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1063) <223> FRXA00290 <400> 581 agctacagat ttagctagtg tttttgttcc agaaccctaa atgaggttct acccttaaca gagcttcccg caaaaacacc gattaacaag gctaaatgat atg acc atc gac ctg 115 IVet Thr lie Asp Leu 1 Page 747 Gene Sequence 24-2-06.txt cag G n gag G u cgc Ar g gca Al a aac Asn gat Asp ct g Leu gtt Val tct Ser gag G u 150 gat Asp t cc Ser t cc Ser gct Al a gac Asp 230 acc Thr gcg Al a tcc acc caa aac ctc acc cat gag gaa Ser Thr Gn Asn Leu Thr His G u u atc ttc gag gca cac Ile Phe u Ala His gga Gy ctt Leu aag Lys gt c Val gga Gy agc Ser gat Asp 120 ggt Gy gag G u cag G n aag Lys gca Al a 200 gca Al a cgt Ar g cct Pr o ct g Leu ct c Leu ctt Leu gat Asp gtt Val cag G n ttc Phe gac Asp at c SIle cgc Ar g ggc Gy 170 ct g Leu gca Al a gac Asp gat Asp ggc Gy 250 at t SIle t cc Ser gct Al a cca Pr o at t SIle 75 gcc Al a gct Al a gct Al a aac Asn ct c Leu 155 acc Thr gat Asp gcg Al a at c SIle ctt Leu 235 at c SIle ggc Gy at t SIle t ac Tyr gag G u t cc Ser t cc Ser ggc Gy ttg Leu ttg Leu 140 at c SIle gct Al a cgc Ar g ggc Gy gt g Val 220 t cc Ser agc Ser gt g Val t cc Ser cct Pr o gcg Al a ggc Gy ccc Pr o aag Lys 110 gag G u gac Asp cgt Ar g gtt Val at c SIle 190 gca Al a ctt Leu gtt Val ggc Gy ggc G0y 270 act Thr ggt Gy cgc Ar g acc Thr gt c Val 95 gct Al a acc Thr at c SIle ct c Leu at c SIle 175 gaa G u gct Al a gat Asp aag Lys at c SIle 255 ggc Gy cgt Ar g gtt Val acc Thr gct Al a 80 at g bet at c l Ie at c SIle t cc Ser gat Asp 160 ct c Leu gac Asp gt a Val t cc Ser gct Al a 240 aat Asn aac Asn ccg Pr o gct Al a cac Hi s gtt Val gag G u cct Pr o gca Al a gct Al a 145 at t SIle gct Al a ct c Leu gat Asp cga Ar g 225 gct Al a gag G u at c SIle ct c Leu cag G n acg Thr ctt Leu ggc G y at c SIle gcc Al a 130 cct Pr o cca Pr o gcg Al a aag Lys at g Net 210 ggc G y ctt Leu gct Al a ggc G y cgc Ar g gtt Val ggc G y ggc G y aag Lys gtt Val 115 at c l Ie cgt Ar g gtt Val ct g Leu at t SIle 195 ct g Leu at c SIle gca Al a ttc Phe gag G5u 275 gac Asp tgt Cys at t SIle ctt Leu gct Al a 100 ttg Leu gcg Al a tgc Cys at g bet cgc Ar g 180 gtt Val acc Thr at c SIle gag G u acc Thr 260 gac Asp at g Mt gaa G u gga G y ggc G y cag G n gac Asp cct Pr o ttc Phe cac Hi s 165 aac Asn att SIe aac Asn cac Hi s aag Lys 245 ggc G y gct Al a Page 748 ctc aaa ctc atg gcc ccg gag cca a Leu Lys Leu I'b t Al a Pro G31u Pr o 1 280 285 acc cca gag at c gat cct gag ctg g Thr Pr o 31u Ilie Asp Pr o G31u Leu S 295 300 gcg acc ggg ccg gtc ttg acc tgc c Al a Thr G31y Pr o Val Leu Thr Cys L 310 315 <210> 582 <211> 321 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn Sequence 24-2- 06. txt .tc ctg ttc acc ctg gcg aac cca le Leu Phe Thr Leu Ala Asn Pro 290 ct cag aag tac ggc gcc atc gtc ~er 03 n Lys Tyr 0 y Al a Hie Val 305 ta acc aga tca .eu Thr Ar g Ser 320 979 1 027 1 063 <400> 582 vb t le Pr o Al a Hi s ValI (31u Pr o Al a Al a 145 li1e Al a Le u As p Ar g 225 Al a Thr I Ie Asp Phe G3 u Al a Leu Arg Asp 31n Va I Cys Thr G3 y Il e Leu G3 y Leu a3 y Lys Al a 1 00 Ilie Val Leu 115 Al a IlIe Al a 130 Pr o Ar g Cys Pr o Val I'b t Ala Leu Arg 1 80 Lys IlIe Val 1 95 I'b t Leu Thr 210 31y Ilie Ilie Leu Al a a3 u Le u Hi s vb t a31u a31y a31y a31n As p Pr o Ph e Hi s 1 65 As n I Ie As n Hi s Ly s 245 a31n Ar g Se r a31u Ar g Al a As n 70 As p Le u ValI Ser a31u 150 As p Ser Ser Al a As p 230 Thr a31y As p lIe Thr I Ie Ph e Hi s Ph e 135 ValI As p Le u a31y a31y 215 Ser As n a31y Le u 40 Ly s ValI a31y Ser As p 120 a31y a31u a31n Ly s Al a 200 Al a Ar g Pr o Th r a3 n 10 Lys Leu Ser Leu a31uAs p Al a Val Pro a31n 90 Ser Phe 1 Val As p Al a I I e Ar g Ar g Hi s a3 y 1 70 Leu Leu 1 a3 y Al a Thr As p a31uAs p Ar g a31y 250 As n Ser Al a Pr o I Ie Al a Al a Al a As n Le u 1 Thr As p Al a I Ie Le u 235 li1e Thr Ser Thr ValI As p Le u Le u ValI 125 a31u a31u ValI Ly s ValI 205 ValI Pr o a31y a31u Thr G y Ar g Thr ValI Al a Thr I Ie Le u I Ie 1 a31u Al a As p Ly s I Ie 255 a31u Al a Phe Thr a31y Al a Asp Leu Phe I Ie a31y Val Ser a31y a31y Asn Page 749 Gene Sequence 24-2-06.txt 260 265 270 lie Gy G u Asp Ala Leu Lys Leu IVet Ala Pro G u Pro lie Leu Phe 275 280 285 Thr Leu Ala Asn Pro Thr Pro G u Ile Asp Pro G u Leu Ser G n Lys 290 295 300 Tyr y Ala Ile Val Ala Thr y Pro Val Leu Thr Cys Leu Thr Arg 305 310 315 320 Ser <210> 583 <211> 582 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> RXN03101 <400> 583 atc ctt gca I e Leu Al a 1 cgc at c ggt Arg lie G y gct cct gcc Al a Pro Al a gca gct cct Al a Al a Pr o atg cca gaa MVt Pro G u ct c aag gct Leu Lys Al a gtc tcc acc Val Ser Thr acc at c gt g Thr Ile Val 115 gca gt c at c Ala Val lle 130 gaa gag gaa Gu Gu u 145 cca aag aag Pro Lys Lys gaa Gu 5 gca Al a gag Gu gct Al a ggc Gy ggc Gy aag Lys at c SIle cgc Ar g gct Al a gag Gu gac Asp aac Asn gaa Gu cca Pr o gaa Gu 70 gac Asp gt c Val ctt Leu at c SIle cct Pr o 150 ccc Pr o gac Asp gca Al a gaa Gu gca Al a 55 t cc Ser acc Thr gac Asp gca Al a ggt Gy 135 gcc Al a aag Lys acc Thr gct Al a cca Pr o 40 gca Al a gt c Val gt c Val acc Thr gac Asp 120 gac Asp gaa G u aag Lys gtc gac Val Asp 10 gca gca Al a Al a gtt aag Val Lys act ggc Thr G y acc gaa Thr G u gaa gta G u Val 90 gaa at c G u Ile 105 gaa gac Su Asp gca aac Al a Asn gag gag G u G u gaa gag Gu Gu gt c Val cct Pr o gaa Gu gcc Al a ggc Gy 75 gac Asp cca Pr o gac Asp gca Al a gaa G u 155 ggc Gy gcc Al a gag Gu gca Al a acc Thr gaa G u t cc Ser acc Thr gct Al a 140 cca Pr o gca Al a gaa Gu cca Pr o acc Thr at t SIle cca Pr o cca Pr o gt c Val 125 gca Al a gtt Val gt c Val gag Gu aag Lys gat Asp acc Thr ctt Leu gt a Val 110 gac Asp gca Al a aag Lys at c I e gaa Gu aag Lys gt g Val cag G n ctt Leu gca Al a gt c Val cct Pr o gaa G u gcc Al a gca Al a gag Gu gaa Gu tgg Tr p gag G u ggc Gy ggc Gy gcc Al a gag G u 160 ccc aag aag gaa gca gct Pro Lys Lys u Ala Ala Page 750 Gene Sequence 24-2-06.txt 165 170 175 act aca cct gct gcg gca tcc gca act gtg tcc gct tct ggc gac aac Thr Thr Pro Ala Ala Al a Ser Ala Thr Val Ser Ala Ser Gy Asp Asn 180 185 190 gtt cca Val Pr o <210> 584 <211> 194 <212> PRT <213> Corynebacter <400> 584 i um gl ut ai cum I e Leu Ala Asp G u Asp Asp Thr 1 Ar g Al a Al a vet Leu Val Thr Al a G u 145 Pr o Gy Al a Pr o G u Al a Thr Val 115 l le G u Lys 5 Al a G u Al a Gy Gy Lys SIle Ar g Al a G u 165 Al a G u Al a Ser Thr Asp Al a Gy 135 Al a Lys Al a Pr o 40 Al a Val Val Thr Asp 120 Asp G u Lys Val Asp 10 Al a Al a Val Lys Thr G y Thr G u G u Val 90 G u Ile 105 Su Asp Al a Asn Su G u G u G u 170 Val Pr o G u Al a Gy 75 Asp Pr o Asp Al a G u 155 Pr o Gy Al a G u Al a Thr G u Ser Thr Al a 140 Pr o Lys Al a G u Pr o Thr SIle Pr o Pr o Val 125 Al a Val Lys Val G u Lys Asp Thr Leu Val 110 Asp Al a Lys G u I e G u Lys Val G n Leu Al a Val Pr o G u Al a 175 Al a Al a G u G u Tr p G u Gy Gy Al a G u 160 Al a Thr Thr Pro Ala Ala Ala Ser Ala Thr Val Ser Ala Ser 180 185 Val Pro G y Asp Asn 190 <210> 585 <211> 540 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(517) <223> RXN02046 <400> 585 Page 751 Gene Sequence 24- 2- 06. txt tccgtgccat caccaccggc gagatcactg gcatcgtgga cgcaaaacaa acagcaacag aaattattaa catccgacgc aacgcttcag gagagtcctc atg aaa gag aca ctg IVet Lys G u Thr Leu acc acc ggt tta acc cac caa atg acc Thr Thr G y Leu Thr His G n et Thr aca gtt ccg cat ctg ctt ccc gaa gca Thr Val Pro His Leu Leu Pro G u Ala 30 gat gtc ctg gcc act gga tat atg gtc Asp Val Leu Ala Thr G y Tyr et Val 45 atg gaa ctt ctg cgt ccc cat ttg gac IVet G u Leu Leu Arg Pro His Leu Asp 60 act cat gtg aac ttc tcc cac gca gct Thr His Val Asn Phe Ser His Ala Ala 75 gtc acc atc gat gtt gag gtg aca gag Val Thr lie Asp Val G u Val Thr G u ttc aac atc act gca gct gat gag ttc Phe Asn Ile Thr Ala Al a Asp G u Phe 105 110 cac cag cgc ggt gtg gtt aac cgt gag His G n Arg G y Val Val Asn Arg G u 120 125 gaa gca cct aag gaa aac taaatcatgg G u Ala Pro Lys G u Asn 135 <210> 586 <211> 139 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 586 IVet Lys G u Thr Leu Thr Thr y Leu 1 5 Val Pro Ala Asn Arg Thr Val Pro His 25 Phe G u Thr IVet Pro Asp Val Leu Ala 40 lie G u Trp Ala Cys IVet G u Leu Leu G u IIe Ser Leu G y Thr His Val Asn 70 Val Pro y Ser Thr Val Thr lie Asp Arg Arg Ala Val Thr Phe Asn Ile Thr 1 t ac at a gt g Tyr Ile Val 15 gca gaa ttt Al a G u Phe ggc atc at c G y lie lle gac ggt gaa Asp G y u cca acg gtt Pro Thr Val at c aac cgt lie Asn Ar g 95 gcc acc at c Ala Thr Ile aag ttt gtc Lys Phe Val cca Pr o gaa G u gag G u at c SIle cct Pr o cgt Ar g agc Ser t cc Ser 130 gca Al a acc Thr tgg Tr p tcg Ser gga Gy gca Al a acc Thr 115 cgt Ar g aac Asn at g bet gcc Al a ctg Leu t cc Ser gtt Val 100 ggc Gy ctg Leu ccaagttgtt tga Thr His 10 Leu Leu I Thr G y Arg Pro I Phe Ser I Val Gu 90 Al a Al a Page 752 bet G u Net Leu Al a Thr G u Thr Al a Val Asp Al a G u Phe Tyr Al a Gy Asp Pr o SIe Al a Gene Sequence 24-2-06.txt 100 105 110 I I e Ser Thr G y Thr His G n Arg G y Val Val Asn Arg G u Lys Phe 115 120 125 Val Ser Arg Leu Pro G u Ala Pro Lys G u Asn 130 135 <210> 587 <211> 1683 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1660) <223> RXN00389 <400> 587 ccaccactgc gtaacctttc cgagcaagat atcgcggacc tgtcggattt gcttgccacc tctggcgcag gt t cctaccg cct t cagt t g aggtgaaagc atg atc acc gca acc lMt lie Thr Ala Thr gca Al a aat Asn cct Pr o tcg Ser aga Ar g ggc Gy cga Ar g gca Al a gga Gy gcg Al a 150 cat Hi s gag Gu t ac Tyr aag Lys gat Asp at c SIle aca Thr acc Thr 120 cgg Ar g ttc Phe tgt Cys aca Thr tta Leu gct Al a ct g Leu caa G n gaa G u aga Ar g gat Asp gca Al a t ca Ser gga Gy gca Al a ttt Phe gat Asp 75 cgg Ar g gt a Val agc Ser ctt Leu agc Ser 155 ct g Leu ttc Phe aac Asn gaa G u t cc Ser gcg Al a gcc Al a gga Gy cgc Ar g 140 aac Asn gat Asp ccg Pr o gca Al a t ac Tyr gct Al a ct g Leu acc Thr 110 ttc Phe cgt Ar g ccc Pr o gag G u acc Thr ct g Leu ct c Leu aac Asn aca Thr aac Asn cga Ar g gtt Val gct Al a 160 gt c Val gcg Al a gcc Al a act Thr gat Asp ttg Leu ct c Leu cgc Ar g 130 ttg Leu tct Ser gct Al a agt Ser gcc Al a cca Pr o gcg Al a gga Gy cgc Ar g 115 at t SIle gga Gy act Thr gga Gy ctg Leu aca Thr gag G u ct a Leu act Thr 100 ctg Leu gaa G u cca Pr o gct Al a ggc gat aca gca tca gcg Sy Asp Thr Ala Ser Al a ttg gct gca ggc tgc Leu Al a Al a G y Cys cct gtg gtt ttt aag Pro Val Val Phe Lys Page 753 Gene Sequence 24-2-06.txt gcg Al a cgg Ar g gt c Val aat Asn 230 ct g Leu gaa G u gat Asp acc Thr ccg Pr o 310 ttt Phe gca Al a at c SIle gt g Val cag G n 390 ccg Pr o gcc Al a at c SIle aat Asn gcc Al a 200 ggc Sy acg Thr cag G n agt Ser gat Asp 280 agt Ser ggt Sy gaa G u cag G n gcc Al a 360 ttt Phe gaa G u caa G n at c l Ie ct c Leu cac Hi s gaa G u ggc Sy at c SIle gcg Al a 250 acc Thr tcg Ser ggg Sy gtt Val acg Thr 330 gga Sy ggc Sy agt Ser ttc Phe cac Hi s 410 gca Al a gag G u cct Pr o aag Lys gt g Val ggt Sy 235 ttt Phe aac Asn agt Ser caa G n ggt Gy 315 ggt Gy gt c Val acc Thr gat Asp ggt Gy 395 caa G n t cc Ser gat Asp gga G y cat Hi s gaa G u 220 ttt Phe agc Ser cct Pr o t cc Ser ttg Leu 300 gat Asp caa G n gac Asp ccc Pr o gt g Val 380 gcg Al a gt a Val cag G n ct c Leu aca Thr gag G u 205 at t SIle acc Thr cgc Ar g gt g Val ttg Leu 285 tgc Cys gct Al a acg Thr aac Asn gga Sy 365 cag G n gca Al a gcc Al a gat Asp gcg Al a 175 gct gag ct c Al a G u Leu 190 ttt gat gct Phe Asp Al a ggc cag gag Sy n u ggt tca cgc G y Ser Arg 240 cca gt t ccc Pro Val Pro 255 ttc gtc ttc Phe Val Phe 270 gct gag gcg Ala G u Al a acc aag cct Thr Lys Pro ttt gtg gcg Phe Val Al a 320 at g ct c acg IVet Leu Thr 335 ctt gca gca Leu Al a Al a 350 gat gga gag Asp G y u gcg ttg cta Ala Leu Leu tcg ctg gtg Ser Leu Val 400 aat t ca ct c Asn Ser Leu 415 gat ttc cag Asp Phe G n 430 ggc cgt gtt G y Arg Val gt c Val ggt Sy ct g Leu 225 cag G n gtt Val ccc Pr o ttt Phe ggc G y 305 ct c Leu caa G n cag G n aac Asn aat Asn 385 gt g Val gag G u gaa G u ggg G y gt g Val 210 gct Al a ggt Sy cca Pr o ggc Sy acc Thr 290 ct c Leu gt a Val ggc G y cca Pr o gcg Al a 370 aat Asn cgt Ar g gga Sy gt c Val caa G n 195 ttt Phe gcg Al a ggt Sy gt c Val gcg Al a 275 gct Al a gtt Val gca Al a at c SIle agt Ser 355 ccg Pr o gt g Val t at Tyr caa G n tcg Ser 435 gcg Al a aac Asn gat Asp ttg Leu ttt Phe 260 ct g Leu t cc Ser ttc Phe gcc Al a gct Al a 340 gt a Val ggc Sy gt g Val gat Asp tta Leu 420 aaa Lys gt g Val ctt Leu ccg Pr o gca Al a 245 gca Al a gcg Al a gt c Val at c SIe aaa Lys 325 cag G n aaa Lys ccg Pr o ttg Leu t cc Ser 405 aca Thr ctt Leu 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 ctt tac ggc ggc tgg Leu Tyr y y Trp Page 754 cca Pr o ccg Pr o 470 gag G u ctt Leu at a SIle 440 ggt 3 y acc Thr ttt Phe gat Asp cgt Ar g 520 Gene Sequence 24-2-06.txt 445 450 gtg gaa gtt ggg cac acg gtt atc cat gga ggc Val G u Val G y His Thr Val IIe His G y G y 460 465 tca aat gcg cag tcg aca agt gtt gga acc ctg Ser Asn Al a G n Ser Thr Ser Val y Thr Leu 475 480 atg cgc ccg gtt tct tat caa act ttc ccg gct Met Arg Pro Val Ser Tyr G n Thr Phe Pro Ala 490 495 cca gtt tct gag gcg aat aaa tgg gct gta cct Pro Val Ser G u Ala Asn Lys Trp Ala Val Pro 505 510 515 taatagctgg tctttacatt tgc cct tat Pro Tyr gca at c Ala I e 485 gag ctg G u Leu 500 cgg gaa Arg G u 1507 1555 1603 1651 1683 <210> 588 <211> 520 <212> PRT <213> Corynebacteri um gl utam cum <400> 588 Met Ile Thr Ala Thr Al a Leu His G y 1 Tr p Asn Ar g Thr I le Gy G n Val Pr o 145 Phe Pr o Val Al a Ser Al a Pr o Al a Gy Ar g 115 I le Sy Thr Val G n 195 5 Lys Asn Thr Val Ser Thr Phe Ar g Val Sy 165 Lys Val Asn Pr o Ser Ar g 70 Sy Ar g Al a Sy Al a 150 Sy Al a Ar g Sy Ser Al a Al a G u Leu G u Pr o 135 Val Asp Hi s Sy I e Ser Ar g Phe Val Sy 105 Val SIle Sy Al a Al a 185 Val Ser Sy Al a Phe Asp Ar g Val Ser Leu Ser 155 Al a Pr o Leu Phe Asn G u Ser Al a Al a Sy Ar g 140 Asn Leu Sy SIle Asp Ser Ser SIle Ser Ar g G n 125 G n Phe Al a Thr Asp Pr o Al a Tyr Al a Leu Thr 110 Phe Ar g Pr o Al a Al a 190 Sy Ar g G n Ar g Asp G u Ser Hi s G n Val Sy 175 G u O u Lys His G u Phe Asp Ala 205 Page 755 Gy Val Phe Asn Leu Val 210 Leu 225 G n Val Pr o Phe Sy 305 Leu G n G n Asn Asn 385 Val G u G u Leu Hi s 465 Gy Phe Al a Al a Gy Val Al a 275 Al a Val Al a SIle Ser 355 Pr o Val Tyr G n Ser 435 Sy Sy Leu Al a Pr o 515 Pr o Al a 245 Al a Al a Val SIle Lys 325 G n Lys Pr o Leu Ser 405 Thr Leu Tr p Tyr SIle 485 Leu G u Asn 230 Leu G u Asp Thr Pr o 310 Phe Al a SIle Val G n 390 Pr o Al a SIle Pr o Pr o 470 G u Leu SIle Tyr 215 I le Ser lbt Leu Sy 295 Ar g Lys Tr p Leu Val 375 G u Asp Thr Pr o Thr 455 Al a Ar g Pr o Asp Gene Sequence 24-2-06. t xt Gy Arg Gy Val Gu Ile Gy Gn Gu Al a Thr Al a 265 Al a Ser Val Thr Ar g 345 G n Su I e Leu Hi s 425 Leu Val Ser let Pr o 505 l Ie Al a 250 Thr Ser Sy Val Thr 330 Sy Sy Ser Phe Hi s 410 Al a G u G u Asn Ar g 490 Val Sy 235 Phe Asn Ser G n Sy 315 Sy Val Thr Asp Sy 395 G n Ser Asp Val Al a 475 Pr o Ser Thr Ar g Val Leu 285 Cys Al a Thr Asn Sy 365 G n Al a Al a Asp Al a 445 Hi s Ser Ser Al a Sy Pr o Phe 270 Al a Thr Phe let Leu 350 Asp Al a Ser Asn Asp 430 Sy Thr Thr Tyr Asn 510 Ser Val 255 Val G u Lys Val Leu 335 Al a Sy Leu Leu Ser 415 Phe Ar g Val Ser G n 495 Lys Ar g 240 Pr o Phe Al a Pr o Al a 320 Thr Al a G u Leu Val 400 Leu G n Val SIe Val 480 Thr Tr p <210> 589 <211> 1467 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS Page 756 Gene Sequence 24-2-06.txt <222> (101)..(1444) <223> RXN02399 <400> 589 ttgacgcacc aatgcccgat ggagcaatgt gtgaaccacg catcacgtcg aaacagtgac agtgcattag ctcatacttt ccaccacgca aaccgatgca gtg gtc ggc acc gcc Val Val y Thr Ala cat Hi s aag Lys cgt Ar g ct g Leu gag G u gcg Al a ggc Sy aac Asn t cc Ser gat Asp 150 gt c Val gt c Val cac Hi s ggc Sy gcc Al a gaa G u cgt Ar g aac Asn ggt Sy ct c Leu ggc Sy aag Lys t cc Ser 120 cca Pr o at c SIle at c SIle gaa G u gaa G u 200 gtt Val ctt Leu gca Al a gca Al a at c SIle gt c Val gac Asp ct c Leu gt c Val cac Hi s gt c Val cgc Ar g gcg Al a 170 cag G n cag G n at c SIle gca Al a ctt Leu cag G n acc Thr at c SIle gca Al a 75 acc Thr tac Ty r acc Thr gtt Val acc Thr 155 gac Asp aag Lys ct c Leu cca Pr o gac Asp aag Lys gaa G u cgc Ar g gag G u gt c Val ggt G y ct g Leu t ac Tyr cgt Ar g 140 gaa G u ggc Sy gca Al a gct Al a acc Thr 220 gtt Val gt g Val cag G n t ac Tyr cac Hi s cag G n cag G n ggt G y 110 gac Asp at c SIle gac Asp gct Al a at c l Ie 190 gaa G u cag G n aac Asn act Thr 15 cag G n acc Thr act Thr gaa G u gct Al a 95 tgg Tr p cag G n aac Asn acc Thr ggc Sy 175 gca Al a aag Lys cac Hi s ttg Leu gat Asp gca Al a ctt Leu ggt G y 80 gtt Val cag G n t cc Ser aac Asn t cc Ser 160 ttc Phe gct Al a aag Lys at c SIle t ca Ser gac Asp cag G n cgc Ar g gga G y cag G n gca Al a t ac Tyr 130 ct g Leu gac Asp gga Sy gct Al a ggc Sy 210 acc Thr aac Asn acc Thr gt a Val cgc Ar g t ac Tyr gtt Val ggt G y 115 cca Pr o ct g Leu aac Asn gca Al a gca Al a 195 cac Hi s ct g Leu gtt Val aac Asn gct Al a ggc Sy at c SIle cgt Ar g 100 gac Asp gcg Al a cgt Ar g tgg Tr p ct c Leu 180 ggc Sy ct c Leu aac Asn gga Sy cct Pr o gat Asp t ca Ser aac Asn gca Al a gcc Al a aac Asn t cc Ser gtt Val 165 aac Asn acc Thr ggc Sy tct Ser acc cca act gtt gtt atc gca Thr Pro Thr Val Val I e Ala Page 757 Gene Sequence 24-2-06.txt 230 235 240 245 cgt acc gac gct gag gca gca acc ctg atc acc tct gac gtt gat gag 883 Arg Thr Asp Ala G u Al a Ala Thr Leu Ile Thr Ser Asp Val Asp G u 250 255 260 cgc gac caa cca ttc atc acc ggt gag cgc acc gca gaa ggc tac tac 931 Arg Asp G n Pro Phe Ile Thr Gy G u Arg Thr Ala G u y Tyr Tyr 265 270 275 cac gtc aag aat ggt ctc gag cca tgt atc gca cgt gca aag tcc tac 979 His Val Lys Asn y Leu G u Pro Cys lie Ala Arg Ala Lys Ser Tyr 280 285 290 gca cca tac gca gat atg atc tgg atg gag acc ggc acc cct gac ctg 1027 Ala Pro Tyr Ala Asp IVet lie Trp IVet G u Thr y Thr Pro Asp Leu 295 300 305 gag ctc gct aag aag ttc gct gaa ggc gtt cgc tct gag ttc cca gac 1075 G u Leu Ala Lys Lys Phe Ala G u y Val Arg Ser G u Phe Pro Asp 310 315 320 325 cag ctg ctg tcc tac aac tgc tcc cca tcc ttc aac tgg tct gca cac 1123 G n Leu Leu Ser Tyr Asn Cys Ser Pro Ser Phe Asn Trp Ser Ala His 330 335 340 ctc gag gca gat gag atc gct aag ttc cag aag gaa ctc ggc gca atg 1171 Leu G u Al a Asp G u I e Al a Lys Phe G n Lys G u Leu G y Al a IVet 345 350 355 ggc ttc aag ttc cag ttc atc acc ctc gca ggc ttc cac tcc ctc aac 1219 G y Phe Lys Phe G n Phe IIe Thr Leu Ala G y Phe His Ser Leu Asn 360 365 370 tac ggc atg ttc gac ctg gct tac gga tac gct cgc gaa ggc atg acc 1267 Tyr Gy IVet Phe Asp Leu Ala Tyr y Tyr Ala Arg G u Gy IVet Thr 375 380 385 tcc ttc gtt gac ctg cag aac cgt gag ttc aag gca gct gaa gag cgt 1315 Ser Phe Val Asp Leu G n Asn Arg G u Phe Lys Ala Ala G u G u Arg 390 395 400 405 ggc ttc acc gct gtt aag cac cag cgt gag gtt ggc gca ggc tac ttc 1363 G y Phe Thr Ala Val Lys His G n Arg G u Val G y Ala G y Tyr Phe 410 415 420 gac cag atc gca acc acc gtt gac ccg aac tct tct acc acc gct ttg 1411 Asp n Ile Ala Thr Thr Val Asp Pro Asn Ser Ser Thr Thr Ala Leu 425 430 435 aag ggt tcc act gaa gaa ggc cag ttc cac aac taggacctac aggttctgac 1464 Lys G y Ser Thr G u G u G y G n Phe Hi s Asn 440 445 aat 1467 <210> 590 <211> 448 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 590 Val Val G y Thr Ala His Cys G u Ser Ala Leu Lys G u Val Thr Leu 1 5 10 Iet Ser Asn Val y Lys Pro Arg Thr Ala Gn Gu Ile Gn Gn Asp 25 Page 758 ene Sequence 24- 2- 06. txt Tr p As p A a As p O31n ValI Le u A a 145 ValI O31y O31y cys Ar g 225 Th r Ser A a Ar g O31y 305 Ser As n O31u Ph e Th r ValI Ar g Ty r ValI O31y 115 Pr o Le u As n A a A a 1 95 Hi s Le u ValI ValI O31y 275 Ly s Pr o Ph e Ser O31y 355 Ser As n Pr o Ar g Tr p As n O31y I Ie Th r Ar g Asp Ty r Th r A a As p Ser As n A a A a As n Ser ValI 1 65 As n Thr O31y Ser A a 245 O31u Tyr Tyr Le u As p 325 Hi s vb t As n O31n lie Le u Le u Le u ValI 135 O31u Pr o Tyr Tr p Ly s 215 Ar g Thr As p ValI Pr a 295 Le u Le u O31u Ph e O31y 375 O31y Le u O31y Ly s Ser 120 Pr a I Ie I Ie O31u O31u 200 ValI Le u As p O31n Ly s 280 Tyr A a Le u A a Ly s 360 vb t Ser Tr p A a A a 1 05 G31y Ser A a ValI Le u 1 85 As p Le u A a A a Pr a 265 As n A a Ly s Ser As p 345 Ph e Ph e ValI As p Le u 90 ValI Hi s ValI Ar g A a 1 70 O31n O31n I Ie A a O31u 250 Ph e O31y As p Ly s Tyr 330 O31u O31n As p I Ie A a Thr Tyr Thr ValI Thr 1 As p Ly s Le u Pr a As p 235 A a I Ie Le u vb t Ph e 315 As n I Ie Ph e Le u O31u ValI O31y Le u Tyr Ar g 140 O31u (31y A a A a Thr 220 ValI A a Thr Ou le 300 A a cys A a I Ie A a 380 O31u Thr As n Ser Pr a 125 Ar g O31y (31u vb t Ser 205 O31n A a Thr O31y Pr a 285 Tr p O31u Ser Ly s Thr 365 Tyr Hi s O31n O31n O31y 110 As p I Ie As p A a I Ie 1 O31u O31n As n Le u O31u 270 cys vb t O31y Pr a Ph e 350 Le u O31y Thr O31u A a Tr p O31n As n Thr G31y 1 A a Ly s Hi s Thr I Ie 255 Ar g I Ie O31u ValI Ser 335 O31n A a Ty r Ar g O31u 31y [Vb t Th r Se r Ph e ValI Asp Leu O31n Asn Arg O31u Phe Lys Page 759 Gene Sequence 24-2-06.txt 385 390 395 400 Ala Ala G u G u Arg G y Phe Thr Ala Val Lys His n Arg u Val 405 410 415 G y Al a y Tyr Phe Asp G n I e Al a Thr Thr Val Asp Pro Asn Ser 420 425 430 Ser Thr Thr Al a Leu Lys G y Ser Thr G u G u G y G n Phe Hi s Asn 435 440 445 <210> 591 <211> 1419 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1396) <223> FRXA02399 <400> 591 caaaccgatg cacatcacgt cgaaacagtg acagtgcatt caccgcccat tgcgaatcag cacttaagga agtgactttg agctcatact ttgtggtcgg aag Lys cgt Ar g ct g Leu gag G u gcg Al a ggc Sy aac Asn t cc Ser gat Asp cgt Ar g aac Asn ggt Sy ct c Leu ggc Sy aag Lys t cc Ser cca Pr o 120 at c SIle gca Al a at c SIle gt c Val gac Asp ct c Leu gt c Val cac Hi s gt c Val cgc Ar g gaa G u cgc Ar g gag G u gt c Val ggt G y ct g Leu t ac Tyr cgt Ar g gaa 10u 140 cag G n t ac Tyr cac Hi s cag G n cag G n ggt G y gac Asp 110 at c SIle gac Asp cag G n 15 acc Thr act Thr gaa G u gct Al a tgg Tr p 95 cag G n aac Asn acc Thr gat Asp gca Al a ctt Leu ggt Sy gtt Val 80 cag G n t cc Ser aac Asn t cc Ser at g Met 1 tgg Tr p gac Asp gct Al a gac Asp cag G n gt c Val ct c Leu gca Al a gtt Val 145 tca aac gtt gga Ser Asn Val y gac acc aac cct Asp Thr Asn Pro cag gta gct gat G n Val Ala Asp cgc cgc ggc tca Arg Arg y Ser gga tac atc aac Sy Tyr Ile Asn cag gtt cgt gca G n Val Arg Ala gca ggt gac gcc Al a G y Asp Al a 100 tac cca gcg aac Tyr Pro Ala Asn 115 ctg ctg cgt tcc Leu Leu Arg Ser 130 gac aac tgg gtt Asp Asn Trp Val Page 760 Gene Sequence 24-2-06.txt gtc cca atc gtc gcg Val Pro Ile Val Ala ggc gaa gct ggc G y u Ala y ttc ggt gga gca ctc aac Phe G y G y Al a Leu Asn 150 gt c Val cac Hi s ggc Sy gcc Al a cgt Ar g 230 cgc Ar g cac Hi s gca Al a gag G u cag G n 310 ct c Leu ggc Sy t ac Tyr t cc Ser ggc Sy 390 gac Asp gaa G u gaa G u gtt Val 200 ctt Leu gac Asp caa G n aag Lys t ac Tyr 280 gct Al a ct g Leu gca Al a aag Lys at g Met 360 gtt Val acc Thr at c SIle cag G n 170 cag G n at c SIle gca Al a gag G u ttc Phe 250 ggt Sy gat Asp aag Lys t ac Tyr gag G u 330 cag G n gac Asp ct g Leu gtt Val acc Thr 410 gca Al a gct Al a acc Thr gtt Val 220 gca Al a acc Thr gag G u at c SIle gct Al a 300 tgc Cys gct Al a at c SIle gct Al a aac Asn 380 cac Hi s gtt Val at g Mbt tct Ser cag G n 205 gca Al a acc Thr ggt Gy cca Pr o tgg Tr p 285 gaa G u t cc Ser aag Lys acc Thr t ac Tyr 365 cgt Ar g cag G n gac Asp at c SIle gaa G u 190 cag G n aac Asn ct g Leu gag Gu tgt Cys 270 at g let ggc Sy cca Pr o ttc Phe ct c Leu 350 gga G y gag G u cgt Ar g ccg Pr o gca Al a 175 aag Lys cac Hi s acc Thr at c SIle cgc Ar g 255 at c SIle gag G u gtt Val t cc Ser cag G n 335 gca Al a t ac Tyr ttc Phe gag G u aac Asn 415 160 gct Al a aag Lys at c SIle cca Pr o acc Thr 240 acc Thr gca Al a acc Thr cgc Ar g ttc Phe 320 aag Lys ggc G y gct Al a aag Lys gtt Val 400 tct Ser ggc Gy tgt Cys cgc Ar g act Thr 225 tct Ser gca Al a cgt Ar g ggc Sy tct Ser 305 aac Asn gaa G u ttc Phe cgc Ar g gca Al a 385 ggc G y tct Ser gct Al a ggc G y acc Thr 210 gtt Val gac Asp gaa Gu gca Al a acc Thr 290 gag G u tgg Tr p ct c Leu cac Hi s gaa G u 370 gct Al a gca Al a acc Thr gca Al a cac Hi s 195 ct g Leu gtt Val gtt Val ggc Gy aag Lys 275 cct Pr o ttc Phe tct Ser ggc Sy t cc Ser 355 ggc G y gaa G u ggc G y acc Thr ggc Gy 180 ct c Leu aac Asn at c SIe gat Asp t ac Tyr 260 t cc Ser gac Asp cca Pr o gca Al a gca Al a 340 ct c Leu at g Net gag G u t ac Tyr gct Al a 420 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 Page 761 Gene Sequence 24- 2- 06. txt aag ggt tcc act gaa gaa ggc cag ttc cac aac taggacctac aggttctgac 1416 Lys G y Ser Thr G u G u G y G n Phe His Asn 425 430 aat 1419 <210> 592 <211> 432 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 592 Net 1 Tr p Asp Al a Asp G n Val Leu Al a Val 145 Sy Sy Cys Ar g Thr 225 Ser Al a Ar g Ser Asn Val Asp Thr Asr 2C Sn Val Al Arg Arg G Sy Tyr IIE Sn Val Arc Al a G y Asp Tyr Pro Al E 115 Leu Leu Arc 130 Asp Asn Tr r G y Al a LeL Al a Al a G 18C G y Hi s LeL 195 Thr Leu Asr 210 Val Val I I Asp Val AsF G u G y Tyr 26C Ala Lys Ser 275 Sy 5 Pr o Asp Ser Asn Al a Al a Asn Ser Val Asn 165 Thr Sy Ser Al a G u 245 Tyr Tyr Lys Pro Arg Thr Ar g Leu G u Al a 70 Sy Asn Ser Asp Val 150 Val Hi s Sy Al a Ar g 230 Ar g Hi s Al a Tr p G n SIle 55 Leu Leu Leu Val G u 135 Pr o Tyr Tr p Lys Ar g 215 Thr Asp Val Pr o Sy Ser Tr p Al a Al a Sy 105 Ser Al a Val Leu Asp 185 Leu Al a Al a Pr o Asn 265 Al a Al a 10 SIle Val Asp Leu Val 90 Hi s Val Ar g Al a G n 170 G n SIle Al a G u Phe 250 Sy Asp G n Thr SIle Al a Thr Tyr Thr Val Thr Asp 155 Lys Leu Pr o Asp Al a 235 I le Leu let G u Ar g G u Val Gy Leu Tyr Ar g G u 140 Sy Al a Al a Thr Val 220 Al a Thr Su lie SIle Asp G u Thr Asn Ser Pr o Ar g 125 Sy G u let Ser G n 205 Al a Thr Sy Pr o Tr p 285 G n Thr Thr G u Al a Tr p G n Asn Thr Sy Al a 175 Lys Hi s Thr SIe Ar g 255 I e G u Sy Thr Pro Asp Leu G u Leu Ala Lys Lys Phe Ala G u y Val Arg Page 762 Ser 305 Asn G u Phe Ar g Al a 385 Gy Ser Phe Ser Gy Ser 355 Gy G u Gy Thr Asp Hi s 325 Met Asn Thr Ar g Phe 405 Leu 295 Leu G u Phe Gy Phe 375 Phe G n Gy Gene Sequence 24-2-06. t xt 300 Leu Ser Tyr Asn Cys Ser Pro 315 Ala Asp 3 u I e Ala Lys Phe 330 Lys Phe G n Phe Ile Thr Leu 345 350 IVet Phe Asp Leu Ala Tyr y 360 365 Val Asp Leu G n Asn Arg G u 380 Thr Ala Val Lys His G n Arg 395 lie Ala Thr Thr Val Asp Pro 410 Ser Thr G u G u G y G n Phe 425 430 Ser G n 335 Al a Tyr Phe G u Asn 415 Hi s <210> 593 <211> 2340 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(2317) <223> RXN02404 <400> 593 gttttcacaa ccgttaacgg cgtagccaaa caagaaggat cacaggtcat ctaaaaccca tgctttaaaa ggagccttca ct g Leu cgc Ar g gt a Val tgg Tr p gag G u cac tct Ser gac Asp gaa G u gga Gy ctt Leu aac cag G n ggc Gy gt a Val gcc Al a cgc Ar g t cc act Thr gga Gy ct c Leu gcc Al a cgc Ar g 75 ggc gcc Al a at g Met cct Pr o at c l Ie gat Asp acc gac Asp cag G n cgc Ar g 45 gcc Al a gaa t cu at c aac Asn gtt Val gt g Val cgg Ar g ct g Leu gac gct gga Al a G y 15 gca aaa Al a Lys ggt gtg G y Val gac ct c Asp Leu cag at g G n let 80 caa gag Page 763 tcgcattctt ctggtttagg atg act gaa cag gaa bet Thr G u G n G u 1 act gac agc acc gaa Thr Asp Ser Thr G u gtt ctc tac gac ttt Val Leu Tyr Asp Phe gat gcg gaa aag ttc Asp Ala G u Lys Phe acc cca cgc aac cgc Thr Pro Arg Asn Arg ctt atc gac gac tac Leu IIe Asp Asp Tyr gcg tac gag gat ttc His Ar g Asn Asn Ser G y Thr Gene Sequence 24-2-06.txt Ile Asp Gn u Ala Tyr u Asp Phe 95 100 ct c Leu cgt Ar g ct g Leu gct Al a 150 cca Pr o ggc G y cca Pr o gat Asp aac Asn 230 gca Al a cct Pr o gtt Val gca Al a gga G y 310 at c SIle ggt G y gaa G u caa G n 120 gtt Val tgg Tr p act Thr aag Lys gac Asp 200 aag Lys gaa G u ct g Leu cac Hi s gaa G u 280 gtt Val aac Asn acc Thr gag G u gga G y gt c Val at t SIle t cc Ser ggc G y 170 at c SIle gct Al a gca Al a t ac Tyr gaa G u 250 at c SIle gcg Al a gct Al a ggc G y gag G u 330 gtt Val tac Ty r gat Asp ct g Leu ct c Leu 155 gct Al a gag G u tcg Ser gcc Al a cgt Ar g 235 acc Thr ggc G y at c l Ie gaa G u gaa G u 315 ct c Leu ct g Leu ttg Leu acg Thr aac Asn 140 t ac Tyr gaa G u tgg Tr p cat Hi s cac Hi s 220 ggc G y aac Asn aag Lys acc Thr gac Asp 300 ct g Leu aac Asn cac Hi s gtt Val gaa G u 125 gca Al a gat Asp aag Lys ggt G y gcc Al a 205 at t SIle ttc Phe ggc G y gca Al a acg Thr 285 aag Lys aaa Lys aag Lys ggt G y gag G u 110 at c SIle cgc Ar g gcg Al a ggc G y cgt Ar g 190 gat Asp gga G y acc Thr ct g Leu gac Asp 270 at c SIle acc Thr gaa G u gac Asp cgt Ar g 350 cca Pr o agc Ser gcg Al a t ac Tyr 160 gag G u ttc Phe gag G u agc Ser aac Asn 240 at c SIle act Thr gac Asp ggt G y at g Met 320 gt c Val ct g Leu gaa G u acc Thr ct g Leu 145 ggc G y t ac Tyr ct c Leu aag Lys gt c Val 225 ttc Phe gag G u ggt G y ttc Phe t ac Tyr 305 t cc Ser t ac Tyr ct g Leu gct Al a gca Al a 130 aac Asn acc Thr aac Asn gac Asp t ac Tyr 210 t ac Tyr ctt Leu ct g Leu ct c Leu gaa G u 290 tct Ser aag Lys at t SIle ttc Phe gca Al a 115 gga G y gct Al a aac Asn ccg Pr o agc Ser 195 aac Asn cga Ar g gat Asp cag G n aaa Lys 275 gac Asp aac Asn aac Asn ggc G y gt c Val 355 gaa G u cct Pr o gcc Al a gcc Al a gt c Val 180 gtt Val at c SIle ct g Leu cca Pr o at c SIle 260 gac Asp t cc Ser tgg Tr p gga G y cgc Ar g 340 cgc Ar g at c SIe cag G n aat Asn at c SIe 165 cgc Ar g gt c Val acc Thr aaa Lys gaa G u 245 gat Asp at c SIe gtt Val ttc Phe cgc Ar g 325 aat Asn aac Asn 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 gtt ggt cac ctc atg caa aac cca tcc atc ttg att gat ggc gag gag Page 764 Gene Sequence 24-2-0 Pro Ser Ile Leu Ile 6. txt Val G y His Leu [et G n Asn 360 at c SIle cca Pr o 390 at c SIle acc Thr cac Hi s aac Asn aac Asn 470 gaa G u aag Lys ctt Leu ct c Leu gcc Al a 550 acg Thr gct Al a gca Al a aac Asn gaa G u at t SIle at c SIle gag G u ttg Leu 440 gat Asp ggc G y ggc G y gcc Al a ggc G y 520 gca Al a act Thr t ac Tyr ggc Gy aac Asn 600 tgc Cys at c SIle ccg Pr o aag Lys 410 ttc Phe gtt Val agc Ser ct g Leu at g bet 490 gag G u gct Al a at g bet tgg Tr p ttg Leu 570 cgc Ar g aat Asn t cc Ser gat Asp 380 aac Asn aag Lys cgc Ar g gtt Val at g Net 460 cgc Ar g cgc Ar g aac Asn at c l Ie gag G u 540 cct Pr o gat Asp agc Ser tct Ser ct c Leu 620 365 gct Al a aag Lys cag G n gtt Val at g bet 445 gaa G u acc Thr aag Lys aac Asn ggt G y 525 aag Lys t ca Ser gt g Val ct g Leu gag G u 605 gga G y gt c Val at g bet cac Hi s gag G u 430 gat Asp gtt Val ggc G y gct Al a gtt Val 510 aag Lys aag Lys cca Pr o ttc Phe cgc Ar g 590 gaa G u t ac Tyr ttg Leu cgc Ar g ggc G y 415 gat Asp gag G u gct Al a gat Asp gat Asp 495 gat Asp ggc G y at c SIle act Thr aag Lys 575 aac Asn gag G u gtt Val acc Thr aat Asn 400 cct Pr o ct g Leu gag G u gac Asp gaa G u 480 at g bet gca Al a at g Net ggc G y ggt G y 560 gtt Val at t SIle aag Lys gt g Val act Thr 385 t cc Ser gaa G u ctt Leu cgt Ar g cgc Ar g 465 at c SIle cag G n ggt G y tgg Tr p cag G n 545 gcg Al a caa G n ct c Leu aag Lys cgc Ar g 625 Asp 370 gtt Val cgc Ar g gaa G u gat Asp cgc Ar g 450 ttg Leu cac Hi s acc Thr at t SIle gcg Al a 530 cca Pr o acg Thr gac Asp acc Thr gaa G u 610 tgg Tr p tgt Cys aag Lys gt c Val ct g Leu 435 acg Thr gca Al a acc Thr gca Al a cag G n 515 at g Net cgc Ar g ct g Leu gaa G u at t SIle 595 gag G u gtt Val gcc Al a ggc G y gcg Al a 420 cca Pr o t cc Ser ttc Phe t cc Ser ccg Pr o 500 cgt Ar g act Thr gaa G u cac Hi s ct g Leu 580 t ca Ser at g Net gag G u at c SIe t cc Ser 405 ttc Phe cgc Ar g gt g Val at c SIe at g bet 485 tgg Tr p ggt G y gaa G u ggc G y gca Al a 565 cgt Ar g acc Thr gac Asp cac Hi s Sy u u 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 ggt gtt ggt tgc tcc aag gtt cca gac atc cat gac atc gac ctc at g Page 765 Gene G y Val G y Cys Ser Lys Val Pr o 630 635 gaa gac cgc gca acg ctg cgt att G u Asp Arg Ala Thr Leu Arg lie 650 atc cgc cat gat gtt gtc tcg aag lie Arg His Asp Val Val Ser Lys 665 cga atg gca gtg gtc gtc gac aag Arg Wet Ala Val Val Val Asp Lys 680 685 cgc gat atg gcg ccg aag tac gac Arg Asp IVet Ala Pro Lys Tyr Asp 695 700 aag gac ttg att ttc gaa ggc acc Lys Asp Leu Ile Phe G u G y Thr 710 715 ccc atc ttg cac gca cgc cgc cgc Pro lie Leu His Ala Arg Arg Arg 730 taagcacgct tttcgacgct tac <210> 594 <211> 739 <212> PRT <213> Corynebacter i um gl ut ari cur Sequence 24-2-06.txt Asp i e His Asp Ile Asp 640 t cc tcg cag atg ctg gcc Ser Ser G n IVet Leu Ala 655 gag cag gtc ttg gag tca G u G n Val Leu u Ser 670 675 caa aat gcg ggc gac gag G n Asn Al a y Asp G u 690 gcc tcc ctc gcc ttc cag Ala Ser Leu Ala Phe G n 705 aag tcc cca tcg ggc tac Lys Ser Pro Ser y Tyr 720 gag ttc aaa gca aaa aac G u Phe Lys Ala Lys Asn 735 Leu aac Asn 660 ct g Leu gcc Al a gcg Al a acc Thr 2083 2131 2179 2227 2275 2317 2340 <400> 594 et Thr G 1 Thr Asp SE Val Leu T} Asp Al a G Thr Pro Ar Leu I e A, Al a Tyr G G u Al a Al 11 Thr Al a G 130 Leu Asn Al 145 G y Thr A, u n er Thr yr Asp u Lys g Asn sp Asp u Asp 100 a u 15 y Pro a Al a sn Al a G u 5 G u Phe Phe Ar g Tyr Phe SIle G n Asn l Ie 165 Leu Ar g Val Tr p G u 70 Hi s Leu Ar g Leu Al a 150 Pr o Leu Val Thr Ser Leu Ar g Lys Thr Val 135 Ar g G u Al a G n 10 Al a G y 25 Al a Val Phe Al a Al a Ar g Asn Ser 90 ie G y 105 Asn Val Pro Ile G y Ser Asp y Thr Al a Asp Asn Sy Leu Al a Ar g 75 Sy Tyr Asp Leu Leu 155 G n Ar g Al a G u SIle Val G u 125 Al a Asp Val Val Ar g Leu Asp G u 110 SIle Ar g Al a Al a y Al a Lys G y Val Asp Leu G n bet G n G u G u Pro Ser Ser Phe Al a Leu Tyr 160 Lys G u 175 Al a u Lys y Page 766 Gene Sequence 24-2-06.txt Tyr Leu Lys Val 225 Phe G u Gy Phe Tyr 305 Ser Tyr Leu SIle Thr 385 Ser G u Leu Ar g Ar g 465 I le G n Gy Pr o Ser 195 Asn Ar g Asp G n Lys 275 Asp Asn Asn Sy Val 355 Sy Cys Lys Val Leu 435 Thr Al a Thr Al a G n 515 Arg G y G n Lys Val lie u Trp y Arg G u Phe 190 Val Thr Lys G u 245 Asp SIle Val Phe Ar g 325 Asn Asn G u SIle Ser 405 Phe Ar g Val l Ie Met 485 Tr p Sy Pr o Asp Asn 230 Al a Pr o Val Al a Gy 310 I e Gy Val SIle Pr o 390 I le Thr Hi s Asn Asn 470 G u Lys Leu Leu Sy 215 Ar g l Ie Val Leu Al a 295 Leu Phe Thr Sy Phe 375 Sy Tyr Asn Thr Leu 455 Thr Al a G n Pr o Asp 200 Lys G u Leu Hi s G u 280 Val Asn Thr G u Hi s 360 G u SIle SIle G u Leu 440 Asp Gy Gy Al a Gy 520 185 Gy Leu Ser Leu Pr o 265 Ser Asp Thr Ar g Leu 345 Leu Gy Al a Val Leu 425 Lys Al a Phe Al a Tyr 505 Lys Al a Al a Tyr G u 250 I le Al a Al a Gy G u 330 Val let SIle Pr o Lys 410 Phe Val Ser Leu let 490 G u Al a Ser Al a Ar g 235 Thr Gy l Ie G u G u 315 Leu Leu G n vet G n 395 Pr o Sy Sy SIle Asp 475 Val Asn G n Al a 205 l le Phe Sy Al a Thr 285 Lys Lys Lys Sy Pr o 365 Al a Lys G n Val let 445 G u Thr Lys Asn Sy 525 Asp Sy Thr Leu Asp 270 I le Thr G u Asp Ar g 350 Ser Val et Hi s G u 430 Asp Val Sy Al a Val 510 Lys Val Asp Sy Hi s 255 Lys vet Leu G u Ar g 335 Ser SIe Leu Ar g Sy 415 Asp G u Al a Asp Asp 495 Asp Sy Trp Ala IVet Thr G u Leu Iet Ala G u IVet Leu G u Lys Lys Ile G y Page 767 Gene Sequence 24-2-06.txt G n 545 Al a G n Leu Lys Ar g 625 Asp vet Leu Gy Al a 705 Ser Al a Arg G u G y Leu Hi s Al a 565 G u Leu Ar g 580 I e Ser Thr 595 Su IVet Asp Val u His Asp Leu IVet 645 Al a Asn Tr p 660 Ser Leu G u 675 G u Al a Tyr G n Ala Al a Tyr Thr G u 725 Asn Al a 550 Thr Al a Al a Asn Gy 630 G u SIle Ar g Ar g Lys 710 535 Asn Hi s Al a Pr o Asn 615 Val Asp Ar g et Asp 695 Asp 540 Pro Ser Pro Thr Al a Trp Val 555 Hi s Leu Val 570 Arg Arg Asp 585 Thr Asn Tr p Sn Ser Ile Cys Ser Lys 635 Al a Thr Leu 650 Asp Val Val 665 Val Val Val Ala Pro Lys Ile Phe G u 715 Val Leu G u 605 Gy Pr o SIle Lys Lys 685 Asp Thr Lys Val 575 Asn I e G u Lys Val Val Ile His 640 Ser G n 655 G n Val Asn Ala Ser Leu Ser Pro 720 Phe Lys 735 Pro Ile Leu His Al a 730 Arg Arg Arg G u <210> 595 <211> 2159 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (1)..(2136) <223> FRXA02404 <400> 595 atg cag gtt gca aaa gtt ctc tac gac IVet G n Val Ala Lys Val Leu Tyr Asp 1 5 cct cgc gtg ggt gtg gat gcg gaa aag Pro Arg Val y Val Asp Ala G u Lys 25 atc gcc cgg gac ctc acc cca cgc aac lie Ala Arg Asp Leu Thr Pro Arg Asn 40 gat gaa ctg cag atg ctt atc gac gac Asp G u Leu G n Iet Leu Ile Asp Asp 55 ttt gta acc gaa gcg gta ct c Phe Val Thr u Al a Val Leu 10 ttc tgg tcc gga ttc gcc gcc Phe Trp Ser y Phe Ala Ala cgc gag ctg ctt gct cgc cgc Arg G u Leu Leu Ala Arg Arg tac cac cgc aac aac tcc ggc Tyr His Arg Asn Asn Ser y Page 768 Gene Sequence 24-2-06.txt acc Thr ttg Leu acg Thr aac Asn t ac Tyr gaa Gu 145 tgg Tr p cat Hi s cac Hi s ggc Gy aac Asn 225 aag Lys acc Thr gac Asp ct g Leu aac Asn 305 cac Hi s gac Asp gag G u at c SIle cgc Ar g 115 gcg Al a ggc G y cgt Ar g gat Asp gga Gy 195 acc Thr ct g Leu gac Asp at c SIle acc Thr 275 gaa Gu gac Asp cgt Ar g gag Gu cca Pr o agc Ser gcg Al a t ac Tyr gag Su ttc Phe 165 gag Gu agc Ser aac Asn at c SIle act Thr 245 gac Asp ggt Sy at g bet gt c Val ct g Leu 325 gcg tac gag gat ttc Al a Tyr G u Asp Phe 70 gaa gct gca gaa atc G u Ala Ala G u Ile 90 acc gca gga cct cag Thr Ala Gy Pro G n 105 ctg aac gct gcc aat Leu Asn Ala Ala Asn 120 ggc acc aac gcc atc G y Thr Asn Ala lle 135 tac aac ccg gtc cgc Tyr Asn Pro Val Arg 150 ctc gac agc gtt gtc Leu Asp Ser Val Val 170 aag tac aac atc acc Lys Tyr Asn Ile Thr 185 gtc tac cga ctg aaa Val Tyr Arg Leu Lys 200 ttc ctt gat cca gaa Phe Leu Asp Pro G u 215 gag ctg cag atc gat G u Leu G n Ile Asp 230 ggt ctc aaa gac at c G y Leu Lys Asp Ile 250 ttc gaa gac tcc gt t Phe G u Asp Ser Val 265 tac tct aac tgg ttc Tyr Ser Asn Trp Phe 280 tcc aag aac gga cgc Ser Lys Asn y Arg 295 tac att ggc cgc aat Tyr lie G y Arg Asn 310 ctg ttc gtc cgc aac Leu Phe Val Arg Asn 330 ct c Leu 75 cgt Ar g ct g Leu gct Al a cca Pr o ggc G y 155 cca Pr o gat Asp aac Asn gca Al a cct Pr o 235 gtt Val gca Al a gga G y at c SIle ggt G y 315 gtt Val aaa gaa Lys G u acc caa Thr G n gtt gtt Val Val cgc tgg Arg Trp 125 gaa act G u Thr 140 cag aag G n Lys ctg gac Leu Asp gga aag G y Lys cgt gaa Arg G u 205 at c ct g II e Leu 220 gtc cac Val Hi s ttg gaa Leu G u gct gtt Al a Val ctc aac Leu Asn 285 ttc acc Phe Thr 300 acc gag Thr G u ggt cac G v Hi s at c gga t ac li e G y Tyr aac gt c gat Asn Val Asp cca att ct g Pro Ile Leu 110 ggt t cc ct c G y Ser Leu gat ggc gct Asp G y Al a gt c at c gag Val lie G u 160 ggt gct t cg G y Al a Ser 175 ctt gca gcc Leu Al a Al a 190 t cc t ac cgt Ser Tyr Arg ct g gaa acc Leu G u Thr cca at c ggc Pro lie y 240 tct gcg at c Ser Al a l e 255 gat gct gaa Asp Al a u 270 acc ggc gaa Thr G y G u cgt gag ct c Arg G u Leu ctg gtt ctg Leu Val Leu 320 ct c at g caa Leu Iet G n 335 240 288 336 384 432 480 528 576 624 672 720 768 816 864 912 960 1008 Page 769 Gene Sequence 24-2-06.txt aac Asn gat Asp aac Asn aag Lys 385 cgc Ar g gtt Val at g Met cgc Ar g cgc Ar g 465 aac Asn at c SIle gag G u cct Pr o gat Asp 545 agc Ser tct Ser ct c Leu t cc Ser gt c Val 355 at g bet cac Hi s gag G u gat Asp gtt Val 435 ggc Gy gct Al a gtt Val aag Lys aag Lys 515 cca Pr o ttc Phe cgc Ar g gaa G u t ac Tyr 595 ttg Leu acc Thr aat Asn cct Pr o ct g Leu 405 gag Su gac Asp gaa G u at g Met gca Al a 485 at g Met ggc G y ggt G y gtt Val at t Ile 565 aag Lys gt g Val at t SIle act Thr t cc Ser gaa G u 390 ctt Leu cgt Ar g cgc Ar g at c SIle cag G n 470 ggt Gy tgg Tr p cag G n gcg Al a caa G n 550 ct c Leu aag Lys cgc Ar g gat Asp gtt Val cgc Ar g 375 gaa G u gat Asp cgc Ar g ttg Leu cac Hi s 455 acc Thr at t SIle gcg Al a cca Pr o acg Thr 535 gac Asp acc Thr gaa G u tgg Tr p ggc gag gag Sy u u 345 tgt gcc at c Cys Ala lle 360 aag ggc t cc Lys G y Ser gtc gcg ttc Val Al a Phe ct g cca cgc Leu Pro Ar g 410 acg tcc gt g Thr Ser Val 425 gca ttc at c Al a Phe Il e 440 acc t cc at g Thr Ser IVet gca ccg tgg Al a Pro Tr p cag cgt ggt G n Arg G y 490 atg act gaa IVet Thr G u 505 cgc gaa ggc Arg u y 520 ctg cac gca Leu Hi s Al a gaa ct g cgt G u Leu Ar g att t ca acc Ile Ser Thr 570 gag atg gac Su IVet Asp 585 gtt gag cac Val G u Hi s 600 atc ttc gaa ggc atc atg I e Phe G u G y Ile IVet 350 cca gga att gct ccg cag Pro y lie Ala Pro G n 365 atc tac atc gtg aag cct Ile Tyr Ile Val Lys Pro 380 acc aac gag ctc ttc ggc Thr Asn G u Leu Phe G y 395 400 cac acc ttg aag gtt ggt His Thr Leu Lys Val y 415 aac ctg gat gcc agc atc Asn Leu Asp Ala Ser Ile 430 aac act ggc ttc ctg gac Asn Thr G y Phe Leu Asp 445 gaa gca ggc gcc atg gtg G u Al a y Al a [et Val 460 aag cag gcc tac gag aac Lys G n Ala Tyr G u Asn 475 480 ctt cct ggc aag gct cag Leu Pro y Lys Ala G n 495 ctc atg gca gaa atg ct g Leu IVet Ala G u Iet Leu 510 gcc aac act gca tgg gtt Ala Asn Thr Ala Trp Val 525 acg cac tac cac ttg gtt Thr His Tyr His Leu Val 540 gct gcc ggc cgc cgc gac Ala Ala G y Arg Arg Asp 555 560 gca cca aac acc aat tgg Ala Pro Asn Thr Asn Trp 575 aac aac tgc cag tcc atc Asn Asn Cys n Ser Ile 590 ggt gtt ggt tgc tcc aag G y Val G y Cys Ser Lys 605 1056 1104 1152 1200 1248 1296 1344 1392 1440 1488 1536 1584 1632 1680 1728 1776 1824 Page 770 gtt cca gac atc cat gac atc Val Pro Asp Ile His Asp Ile 610 615 cgt att tcc tcg cag atg ctg Arg lie Ser Ser G n IVbt Leu 625 630 tcg aag gag cag gtc ttg gag Ser Lys G u G n Val Leu G u 645 gac aag caa aat gcg ggc gac Asp Lys G n Asn Ala G y Asp 660 tac gac gcc tcc ctc gcc ttc Tyr Asp Ala Ser Leu Al a Phe 675 ggc acc aag tcc cca tcg ggc Gy Thr Lys Ser Pro Ser G y 690 695 cgc cgc gag ttc aaa gca aaa Arg Arg G u Phe Lys Al a Lys 705 710 <210> 596 <211> 712 <212> PRT <213> Corynebacter i um gl ut a <400> 596 lbt G n Val Ala Lys Val Leu 1 5 Pro Arg Val y Val Asp Al a lie Ala Arg Asp Leu Thr Pro Asp u Leu n IVbt Leu Ile Thr Ile Asp n u Ala Tyr 70 Leu Val G u G u Pro G u Ala Thr G u Ile Ser Ser Thr Al a 100 Asn Ala Arg Phe Ala Leu Asn 115 Tyr Asp Al a Leu Tyr G y Thr 130 135 G u Lys G y Lys G u Tyr Asn 145 150 Trp G y Arg G u Phe Leu Asp Gene Sequence 24-2-06.txt gac ctc atg gaa gac cgc gca acg Asp Leu IVbt G u Asp Arg Ala Thr 620 gcc aac tgg atc cgc cat gat gtt Ala Asn Trp lie Arg His Asp Val 635 t ca ct g gaa cga atg gca gtg gt c Ser Leu G u Arg IVbt Ala Val Val 650 655 gag gcc tac cgc gat atg gcg ccg G u Ala Tyr Arg Asp IVbt Ala Pro 665 670 cag gcg gct aag gac ttg att ttc G n Ala Ala Lys Asp Leu Ile Phe 680 685 tac acc gag ccc atc ttg cac gca Tyr Thr G u Pro lie Leu His Ala 700 aac taagcacgct tttcgacgct tac Asn 1872 1920 1968 2016 2064 2112 2159 Asp Lys 25 Asn Asp Asp G u Pr o 105 Al a Al a Val Val Val Tr p G u Hi s Leu Ar g Leu Al a Pr o Sy 155 G u Sy Leu Asn G u G n Val Tr p 125 Thr Lys Al a Phe Al a Asn SIle Asn Pr o 110 Sy Asp Val Val Al a Ar g Ser Sy Val I e Ser Gy lie Val Pro Leu Asp G y Ala Ser Page 771 Gene Sequence 24-2-06.txt His Ala Asp Val G u Lys Tyr Asn Ile Thr Asp G y Lys Leu Al a Al a 190 Hi s Gy Asn 225 Lys Thr Asp Leu Asn 305 Hi s Asn Asp Asn Lys 385 Ar g Val let Ar g Ar g 465 Asn SIle G u Gy 195 Thr Leu Asp SIle Thr 275 G u Asp Ar g Ser Val 355 let Hi s G u Asp Val 435 Gy Al a Val Lys Lys 515 Ser Asn SIle Thr 245 Asp Gy let Val Leu 325 Leu Thr Asn Pr o Leu 405 G u Asp G u et Al a 485 bet Gy Tyr Leu 215 Leu Leu G u Ser Lys 295 I le Phe Asp Val Ar g 375 G u Asp Ar g Leu Hi s 455 Thr l Ie Al a Pr o Ar g 200 Asp G n Lys Asp Asn 280 Asn Gy Val Gy Cys 360 Lys Val Leu Thr Al a 440 Thr Al a G n et Ar g 520 185 Leu Pr o SIle Asp Ser 265 Tr p Gy Ar g Ar g G u 345 Al a Gy Al a Pr o Ser 425 Phe Ser Pr o Ar g Thr 505 G u Asn Al a Pr o 235 Val Al a Gy SIle Gy 315 Val SIle Pr o SIle Thr 395 Hi s Asn Asn G u Lys 475 Leu Leu Al a Ar g l Ie 220 Val Leu Al a Leu Phe 300 Thr Gy Phe Gy Tyr 380 Asn Thr Leu Thr Al a 460 G n Pr o Aet Asn G u 205 Leu Hi s G u Val Asn 285 Thr G u Hi s G u SIle 365 I le G u Leu Asp Gy 445 Gy Al a Gy Al a Thr 525 Ser Leu Pr o Ser Asp 270 Thr Ar g Leu Leu Gy 350 Al a Val Leu Lys Al a 430 Phe Al a Tyr Lys G u 510 Al a Tyr G u SIe Al a 255 Al a Gy G u Val let 335 I e Pr o Lys Phe Val 415 Ser Leu let G u Al a 495 let Tr p Pro Ser Pro Thr Gy Ala Thr Leu His Ala Thr His Tyr His Leu Val Page 772 Gene Sequence 24-2-06.txt 540 535 Asp 545 Ser Ser Leu Val Ar g 625 Ser Asp Tyr Gy Ar g 705 Phe Ar g G u Tyr 595 Asp Ser G u G n Al a 675 Lys G u Val SIle 565 Lys Val Hi s G n Val 645 Al a Leu Pr o Lys G n 550 Leu Lys Ar g Asp bet 630 Leu Gy Al a Ser Al a 710 Asp G u Leu Arg Thr G u Tr p SIle 615 Leu G u Asp Phe Gy 695 Lys Ser let 585 G u Leu Asn Leu Al a 665 Al a Thr Thr 570 Asp Hi s et Tr p G u 650 Tyr Al a G u Al a 555 Al a Asn Gy G u SIle 635 Ar g Ar g Lys Pr o Asn Cys Gy 605 Ar g Hi s Al a et Leu 685 Leu Ala G y Arg Arg Thr G n 590 Cys Al a Asp Val Al a 670 I le Hi s Asn 575 Ser Ser Thr Val Val 655 Pr o Phe Al a <210> 597 <211> 873 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(850) <223> RXA01089 <400> 597 aaaaattcgg ttcacaaaag gtttatcagt ccagcttaaa attagactca aggcacatca cattgaagga gcttcttatc gcc Al a gat Asp t ac Tyr ct g Leu ct g Leu gct Al a ttc Phe at t SIle ttg Leu aag Lys gtt Val ct g Leu act Thr cat Hi s caa G n tt c Phe ttt Phe gct Al a gag G u aat Asn gag G u agt Ser aaa Lys cca Pr o ct a gat Leu Asp 15 gcc gtg Al a Val cag cgt G n Arg cct ggg Pro G y Page 773 cccatcgcac gggcgggaga ttg tct cga ttt gct Leu Ser Arg Phe Ala 1 ttc ctg gat cgt ttt Phe Leu Asp Arg Phe gag ttt cag tac cct G u Phe G n Tyr Pro gct gat tcc gca ggt Ala Asp Ser Ala G y gat act ttt ggt ctt Asp Thr Phe G y Leu Gene Sequence 24-2-06.txt gcg Al a at c SIle ggc G y at t SIle at t SIle tta Leu 150 aag Lys acc Thr caa G n aat Asn gt c Val 230 ggc Gy ct g Leu t ac Tyr gcg Al a tgg Tr p 120 cca Pr o cag G n tta Leu cgc Ar g gcc Al a 200 gcg Al a gct Al a ttg Leu gct t cc Al a Ser gcc aca Al a Thr gac gta Asp Val 105 gct gcg Al a Al a att aat I e Asn gcg tat Al a Tyr ttc gat Phe Asp 170 ctg cgc Leu Arg 185 tca gt t Ser Val tat at c Tyr Ile ggc gaa G y G u gag ct c G u Leu 250 gaa gac Su Asp ttg aag Leu Lys tca gaa Ser G u caa ct a G n Leu 125 tat tcg Tyr Ser 140 ctt atc Leu II e ttc cac Phe Hi s gtt cat Val Hi s gat cga Asp Arg 205 caa ct c G n Leu 220 cac cct His Pr o ttt caa caa tcc Phe G n G n Ser cca Pr o acc Thr 110 gac Asp gtt Val gac Asp ctt Leu ggc Gy 190 cac Hi s ct a Leu gct Al a aag Lys 95 acg Thr aag Lys ccc Pr o agc Ser cag G n 175 gca Al a gaa G u agc Ser ggt Gy 80 aag Lys gcg Al a ct c Leu ggt G y at t SIle 160 cag G n ggt G y cct Pr o gaa G u gaa G0u 240 atc gag cag gcc lie G u G n Ala cat gtc atg gct His Val Met Ala 100 tat gtg gag aat Tyr Val G u Asn 115 gtt gtc gtt gtt Val Val Val Val 130 ttc ctg cac act Phe Leu His Thr cac ccc aat gtg His Pro Asn Val 165 cat ggc aat ctc Hi s G y Asn Leu 180 ttg gga cac gtg Leu G y Hi s Val 195 act ggc gaa gtc Thr G y G u Val 210 gga tat gac ggt G y Tyr Asp G y aca gcc ggt Thr Al a G y t agat cgt aa gt ggt gt cgt acc <210> 598 <211> 250 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 598 Leu Ser Arg Phe Ala Al a Asn Leu Ser Leu Thr Phe Thr G u Leu Asp 1 5 10 Phe Leu Asp Arg Phe Asp Ala Ala Ser Lys His Ala Phe Ser Ala Val 25 G u Phe G n Tyr Pro Tyr Asp Phe Asp Val On u Ile Lys G n Arg 40 Ala Asp Ser Ala y Leu Pro lie G u Leu Phe Asn Ala Pro Pro y 55 Page 774 Asp Ser Mbet Ar g Asp Tyr 145 Al a SIle Leu Gy Leu 225 Thr Thr Phe y Leu Al a Ala Gene Sequence 24-2-06.txt Leu Ala Ser Pro G u Asp Phe n G n G u Val Val 115 Val Leu Pr o Gy Gy 195 Gy Tyr Al a Al a Al a Asn Val Thr Val 165 Leu Val Val Gy Leu 245 Thr l Ie Ar g G u 135 G u SIle Ar g Val Al a 215 I le Tr p Tyr Al a Tr p 120 Pr o G n Leu Ar g Al a 200 Al a Al a Leu Al a Asp 105 Al a SIle Al a Phe Leu 185 Ser Tyr Sy G u Val Thr G n Hi s Tr p 155 Thr G u Pr o Phe Tyr 235 Lys G u Leu 125 Ser SIle Hi s Hi s Ar g 205 Leu Pr o Pr o Thr 110 Asp Val Asp Leu Sy 190 Hi s Leu Al a Lys Thr Lys Pr o Ser G n 175 Al a G u Ser Gy <210> 599 <211> 897 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(874) <223> RXA01886 <400> 599 gcgaagctgc acgcaccgca ctactcatcg cactcggcgc gcgcaaccat caaccttgct gaaagcatcg aggtttaacc gca Al a gt c Val t cc Ser t cc Ser tgc Cys acg Thr aaa Lys acg Thr gag G u tct Ser at c SIle t ac Tyr at c SIle cag G n gcg Al a cgc Ar g t ac Tyr gga Sy ct c Leu 45 ggg G y cag G n ttc Phe gt g Val gat Asp gac ctg Asp Leu 15 ctc gt g Leu Val gca aca Al a Thr ctg att Leu II e Page 775 catccgaagc gtagaaaccg atg act ttt aaa ctc IVet Thr Phe Lys Leu 1 cct ttc gag gag agg Pro Phe G u G u Arg gaa att tgg gac tgg Su lie Trp Asp Trp ggc gcg gaa ttt tcc G y Al a u Phe Ser act gaa cag ggc cgc Thr Gu Gn Gy Arg Gene Sequence 24- 2- 06. txt 60 gcg gag ctc ttg gca acc gct tcg gag tcc ttg gcg gtg gcg gaa aag 355 Ala G u Leu Leu Ala Thr Ala Ser u Ser Leu Ala Val Ala G u Lys 75 80 ctc aac tgc ccc cgg ctg aat ctg cat gga act ggc ctt gga ccg cag 403 Leu Asn Cys Pro Arg Leu Asn Leu His G y Thr G y Leu G y Pro G n 95 100 gga cta cct gtt act ccc att gaa gtg gtt acc cca gaa atg tgg ctc 451 Sy Leu Pro Val Thr Pro lie G u Val Val Thr Pro G u Met Trp Leu 105 110 115 tac gct gct gaa acg ctc cgc cag atc gct gag ctg ggg gag cgc gca 499 Tyr Ala Al a u Thr Leu Arg On lle Ala u Leu Gy u Arg Ala 120 125 130 ggc aag gtt ttc gtg ctg gaa aac ctc aac ctc gca gtc gat cac ccc 547 Sy Lys Val Phe Val Leu G u Asn Leu Asn Leu Ala Val Asp His Pro 135 140 145 ggc act cct ttt gcc aag gcc act gac act ttg gcg ctg gtc aag gct 595 Sy Thr Pro Phe Ala Lys Ala Thr Asp Thr Leu Ala Leu Val Lys Ala 150 155 160 165 gtc aat cac ccg aat ctg cgc ctc aac ctg gat ttg tac cac gcc cag 643 Val Asn His Pro Asn Leu Arg Leu Asn Leu Asp Leu Tyr His Ala G n 170 175 180 att ggc gaa gga aac ctc att gag ctg ctc cgt gag gcg cag cca ttc 691 lie G y G u G y Asn Leu Ile G u Leu Leu Arg G u Al a n Pro Phe 185 190 195 atc ggc gaa atc cag gtt gcc gat gtc ccc ggc cgc atg gaa ccc ggc 739 Ile G y u Ile G n Val Ala Asp Val Pro y Arg IVet u Pro y 200 205 210 acc ggc gag atc aac tac cag ggc gtc gcg aaa gct ctc gcc gcg atg 787 Thr Gy u lie Asn Tyr n y Val Ala Lys Ala Leu Ala Ala IVet 215 220 225 ggc tac gac ggc gtc atc ggc atg gag gcg tgg gca tcg ggc gac tcc 835 Sy Tyr Asp y Val Ile Gy IVet u Ala Trp Ala Ser y Asp Ser 230 235 240 245 agc gac gcg ctg cag gcg ttg aag tca gcg ttc acg gtc taaattgctt 884 Ser Asp Ala Leu G n Al a Leu Lys Ser Ala Phe Thr Val 250 255 atcgacgcac ccc 897 <210> 600 <211> 258 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 600 IVet Thr Phe Lys Leu Al a Ala Cys Ala G u IVet Ile Tyr G n Asp Leu 1 5 10 Pro Phe u u Arg Val Lys Thr lie Ser Asp G n y Phe Leu Val 25 G u lie Trp Asp Trp Ser Thr Lys Asp Ile Asp Ala Leu Val Ala Thr 40 Page 776 G3 y Al a (3 u Phe Ser Ser I'bt ene Sequence 24- 2- 06. txt Thr Gy Tyr Leu Arg Gy Asp Leu Ilie Thr Al a G31y Pr o Le u Al a 145 Al a Le u O31u Ar g Al a 225 Al a Thr O31n Al a G31y vb t 115 O31u As p ValI Hi s O31n 1 95 O31u Al a Ar g Ly s O31n Le u Al a Pr o Al a 1 65 O31n Ph e O31y vb t Al a 70 Le u O31y Tyr O31y O31y 150 ValI I Ie I Ie Thr O31y 230 O31u As n Le u Al a Ly s 135 Thr As n O31y O31y O31y 215 Tyr Le u Pr o ValI 1 O31u Ph e Ph e Pr a O31y 1 le le Oy Al a Ar g 90 Thr Thr ValI Al a As n 1 70 As n O31n As n ValI Thr Le u Pr a Le u Le u Ly s 1 Le u Le u ValI Tyr I Ie 235 Al a As n I Ie Ar g O31u 140 Al a Ar g I Ie Al a O31n 220 (31y Ser Le u O31u O31n 125 As n Thr Le u O31u As p 205 O31y vb t Ser O31y ValI Al a As n Thr Le u 1 Le u Pr a Al a Al a Se r 03 y As p Se r 245 ValI Ser Asp Al a Leu Al a Leu Lys Ser Al a Phe 255 <210O> 601 <21 1> 1575 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 ODS <222> (1552) <223> RXN03117 <400> 601 t gt gcaacat t agt tcgt ta agaagagt ca cat tccagcc at g att acc cac gaa I'b t I Ie Thr Hi s O31u 1 gtg cgc acc cac cgt tct gcg gaa gag ttc ccg tac aag aag cac ctg Val Ar g Thr Hi s Ar g Ser Al a 31u O31u Phe Pr o Tyr Lys Lys Hi s Leu 15 gct cac aag at g gct cgc gt t gca gcc gac cca gtt gag gt t gct gcg Al a Hi s Lys I'b t Al a Ar g Val Ala Ala Asp Pr o Val O31u Val Al a Al a 30 gac act cag gaa at g at c at c acc cgc at c atc gac aat gca t cg gt g Asp Thr O31n 1u 'b t Ilie Ile Thr Arg Ilie Ile Asp Asn Al aSer Val Page 777 Gene Sequence 24-2-06.txt cag G n gca Al a ct g Leu gcg Al a t cc Ser gct Al a gag G u 150 at t SIle acc Thr gca Al a tct Ser gag G u 230 tgg Tr p cac Hi s at c SIle gca Al a ggc Gy gct Al a gt c Val gga Gy cgt Ar g cca Pr o 120 aaa Lys cag G n cac Hi s ct a Leu cac Hi s 200 tgg Tr p gt a Val ggc Sy t ac Tyr gat Asp 280 at c l Ie gac Asp gt g Val cca Pr o t at Tyr ctt Leu gat Asp ggc Sy aac Asn gct Al a 170 ct a Leu acc Thr gca Al a cgc Ar g gac Asp 250 at t SIle t ac Tyr ttg Leu cgt Ar g tt g Leu gtt Val 75 gcc Al a gat Asp aac Asn aag Lys tt g Leu 155 cat Hi s gat Asp acg Thr ttt Phe gca Al a 235 ggc Sy cct Pr o acc Thr gcg Al a gat Asp cgt Ar g acc Thr gcg Al a ttc Phe at t SIle gat Asp 140 gt g Val ctt Leu gt g Val gcg Al a gct Al a 220 at g Met gt a Val ttg Leu aag Lys cgc Ar g 300 gt g Val cga Ar g gat Asp gaa G u cat Hi s cct Pr o 125 ct g Leu cgt Ar g gga G y gac Asp acg Thr 205 cct Pr o cgt Ar g at c SIle cct Pr o gaa G u 285 agc Ser gac Asp cca gtt agc Pro Val Ser ggt cgg ggt G y Arg G y 80 tgg gct gcg Trp Al a Al a 95 gac acg ttc Asp Thr Phe 110 ccg att ttg Pro Ile Leu atc cgt ggc lie Arg y gga at g t gc G y Met Cys 160 cca t ca gcg Pro Ser Ala 175 acc at c t ac Thr Ile Tyr 190 agg cag tcc Arg G n Ser gcg ttt gcg Al a Phe Al a ggc gag ggc Sy u y 240 gcg tgg ct g Ala Trp Leu 255 gca gaa ggt Al a G u G y 270 cac tcg gcg Hi s Ser Al a atg ggg gag [et y u tcc atc gtt Ser Ile Val tct Ser gca Al a ctt Leu ct c Leu gct Al a at c SIle 145 ct g Leu gct Al a cag G n cgt Ar g ggc G y 225 gca Al a ct g Leu gaa G u gaa G u aag Lys 305 gcc Al a t ct Ser gct Al a gct Al a gca Al a 130 gct Al a cat Hi s gct Al a gca Al a aaa Lys 210 aag Lys ccg Pr o t cc Ser gcc Al a t ac Tyr 290 ct g Leu cgt Ar g gtt Val aac Asn gcg Al a 115 gca Al a act Thr gag G u ggt G y at t l Ie 195 ggt G y at g Net t ca Ser ggt Gy aaa Lys 275 cag G n gca Al a gcg Al a ttc Phe ggc Gy 100 gaa G u cag G n ggg Sy cac Hi s at c SIle 180 ggt G y gcg Al a t cc Ser cca Pr o ctt Leu 260 cga Ar g t ca Ser gca Al a at g bet ggt Sy act Thr t ac Tyr cag G n t at Tyr aag Lys 165 gga G y cag G n at t SIe at c SIe at c SIe 245 gat Asp gca Al a cag G n cag G n 247 295 343 391 439 487 535 583 631 679 727 775 823 871 919 967 1015 ttg cac acc tcc cac Leu His Thr Ser His Page 778 Gene Sequence 24-2-06.txt 310 cac Hi s gat Asp ttc Phe gct Al a at t SIle 390 gat Asp gat Asp ct g Leu acc Thr gat Asp 470 at t SIle gga Gy cac t ac gt g Hi s Tyr Val 330 gat gca t cg Asp Al a Ser 345 gtc gcg ct g Val Al a Leu 360 gag cga gcc G u Arg Ala acg gtg gag Thr Val G u gca gaa aag Al a G u Lys 410 acc gtc gt g Thr Val Val 425 gca cgg cct Al a Ar g Pr o 440 gct gaa ggt Al a G u G y gca cag cgt Al a G n Ar g ttg gat gcc Leu Asp Al a 490 ttc tgatggc! Phe at c SIle cga Ar g aag Lys cac Hi s gat Asp 395 gcc Al a gaa G u tt c Phe gtt Val acg Thr 475 gat Asp ggc Gy gaa G u gat Asp cgc Ar g 380 cct Pr o ttc Phe gat Asp gct Al a gt g Val 460 cac Hi s at t SIle acc Thr acc Thr cgc Ar g 365 cga Ar g gaa G u ggc Gy gaa G u agg Ar g 445 t cc Ser gag G u ttg Leu gga Gy ctt Leu 350 gcg Al a gag G u tgg Tr p gca Al a ct g Leu 430 gag G u gaa G u ctt Leu gcc Al a 320 aat Asn cac Hi s cac Hi s at c SIle agg Ar g 400 gca Al a gt g Val t ac Tyr gaa G u gat Asp 480 gct Al a cca Pr o at c SIle gag G u 370 ct g Leu t ac Tyr at c l Ie aat Asn cag G n 450 gat Asp t ca Ser gt g Val cag G n at g Met 355 cgt Ar g tgg Tr p cac Hi s acc Thr gcg Al a 435 aaa Lys cgc Ar g gaa G u at t SIle aag Lys 340 t ac Tyr t cc Ser aac Asn t cc Ser ttc Phe 420 cat Hi s ttc Phe ttc Phe ct c Leu ccg Pr o 500 1063 1111 1159 1207 1255 1303 1351 1399 1447 1495 1543 1575 gg tttgttttcc tct <210> 602 <211> 504 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 602 lIet Ile Thr His u Val Arg Thr His Arg Ser Ala G u u Phe Pro 1 5 10 Tyr Lys Lys His Leu Ala His Lys IVet Ala Arg Val Ala Ala Asp Pro 25 Val u Val Al A Ala Asp Thr Gn u IVet lie lie Thr Arg lie lie 40 Asp Asn Ala Ser Val G n Ala Ala Ser Val Leu Arg Arg Pro Val Ser 55 Page 779 Gene Sequence 24-2-06.txt Ser Al a Leu Leu Al a SIle 145 Leu Al a G n Ar g Gy 225 Al a Leu Su Su Lys 305 Leu Asp Ser Hi s G u 385 Ar g Ar g Val Asn Al a 115 Al a Thr G u Gy I e 195 Gy bet Ser Gy Lys 275 G n Al a Thr G n Met 355 Ar g Tr p Hi s Ala let Al a n Val Arg Pro Val Thr Asp y Arg Gy Thr Tyr G n Tyr Lys 165 Gy G n SIle SIe SIle 245 Asp Al a G n G n Hi s 325 Phe SI e Tyr Lys Val 405 70 Leu Al a Ser Al a G u 150 I le Thr Al a Ser G u 230 Tr p Hi s l Ie Al a Gy 310 Hi s Asp Phe Al a SIle 390 Asp Pr o Val Hi s Gy 135 I le Asp Leu Leu Ser 215 Al a G u SIle Leu Pr o 295 Leu Thr Pr o Al a Pr o 375 Ser Pr o Gy Ar g Pr o 120 Lys G n Hi s Leu Hi s 200 Tr p Val Sy Tyr Asp 280 I le Asp Hi s Asp Val 360 G u Thr Al a Ar g G u 105 Sy Sy Val Val Asp 185 Thr Lys Asp G u Thr 265 Thr Asp Leu Tyr Al a 345 Al a Ar g Val G u Tyr 90 Leu Asp Sy Asn Al a 170 Leu Thr Al a Ar g Asp 250 I le Tyr Leu Ar g Val 330 Ser Leu Al a G u Lys 410 Al a Asp Asn Lys Leu 155 Hi s Asp Thr Phe Al a 235 Sy Pr o Thr Al a Asp 315 I le Ar g Lys Hi s Asp 395 Al a Al a Phe SIle Asp 140 Val Leu Val Al a Al a 220 Met Val Leu Lys Ar g 300 Val Sy G u Asp Ar g 380 Pr o Phe G u Hi s Pr o 125 Leu Ar g Sy Asp Thr 205 Pr o Ar g SIle Pr o G u 285 Ser Asp Thr Thr Ar g 365 Ar g G u Sy Tr p Asp 110 Pr o SIle Sy Pr o Thr 190 Ar g Al a Sy Al a Al a 270 Hi s let Ser Sy Leu 350 Al a G u Tr p Al a Al a Thr SIle Ar g let Ser 175 I le G n Phe G u Tr p 255 G u Ser Sy SIle Ser 335 Asp Tr p Thr Thr Ar g 415 Sy Al a Phe Leu Sy Cys 160 Al a Tyr Ser Al a Sy 240 Leu Sy Al a G u Val 320 Asn Hi s Hi s SIe Ar g 400 Al a Val lie Thr Phe Lys Asp y Thr Val Val G u Asp G u Leu Ala Val Page 780 Gene Sequence 24-2-06.txt 420 425 430 Ala Asn Ala His Pro Leu G y Ala Arg Pro Phe Ala Arg G u G n Tyr 435 440 445 I le n Lys Phe Arg Thr Leu Al a G u y Val Val Ser G u Lys G u 450 455 460 G n Asp Arg Phe Leu Asp Ala Ala G n Arg Thr His G u Leu G u Asp 465 470 475 480 Leu Ser G u Leu Asn Ile G u Leu Asp Ala Asp Ile Leu Ala Lys Ala 485 490 495 Pro Val IIe Pro G u G y Leu Phe 500 <210> 603 <211> 975 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> <223> FRXA00406 <400> 603 gac cca gtt gag gtt gct gcg gac act cag gaa atg atc atc acc cgc 48 Asp Pro Val u Val Al a Ala Asp Thr n G u IVet lie lie Thr Arg 1 5 10 atc atc gac aat gca tcg gtg cag gca gct tcc gtg ttg cgt cga cca 96 lie lie Asp Asn Ala Ser Val G n Ala Ala Ser Val Leu Arg Arg Pro 25 gtt agc tct gcc cgt gcg atg gca cag gtc agg cca gtt acc gat ggt 144 Val Ser Ser Al a Arg Al a Iet Al a n Val Arg Pro Val Thr Asp G y 40 cgg ggt gca tct gtt ttc ggt ctg cca gga cgt tat gcc gcg gaa tgg 192 Arg y Ala Ser Val Phe y Leu Pro y Arg Tyr Ala Ala G u Trp 55 gct gcg ctt gct aac ggc act gcg gtg cgt gag ctt gat ttc cat gac 240 Ala Ala Leu Ala Asn G y Thr Ala Val Arg G u Leu Asp Phe His Asp 70 75 acg ttc ctc gct gcg gaa tac tcc cac cca gga gat aac att cct ccg 288 Thr Phe Leu Ala Ala G u Tyr Ser His Pro G y Asp Asn lie Pro Pro 90 att ttg gct gca gca cag cag gct gga aaa ggt ggc aag gat ctg atc 336 Ile Leu Al a Al a Al a n G n Al a G y Lys G y G y Lys Asp Leu Ile 100 105 110 cgt ggc atc gct act ggg tat gag att cag gtt aac ttg gtg cgt gga 384 Arg G y lie Ala Thr Gy Tyr u lie n Val Asn Leu Val Arg y 115 120 125 atg tgc ctg cat gag cac aag att gat cac gtt gct cat ctt gga cca 432 IVet Cys Leu His G u His Lys lie Asp His Val Ala His Leu G y Pro 130 135 140 tca gcg gct gct ggt atc gga acc ttg cta gac cta gat gtg gac acc 480 Ser Ala Ala Ala G y I e G y Thr Leu Leu Asp Leu Asp Val Asp Thr 145 150 155 160 Page 781 Gene Sequence 24-2-06.txt at c SIle cag G n ttt Phe gag G u tgg Tr p 225 gaa G u tcg Ser ggg Gy at c l Ie tct Ser 305 gat Asp cag G n cgt Ar g ggc G y 195 gca Al a ct g Leu gaa G u gaa G u aag Lys 275 ttg Leu gat Asp t cc Ser at t SIle 165 ggt Sy at g bet t ca Ser ggt Sy aaa Lys 245 cag G n gca Al a acc Thr cag G n at g 3et 325 ggt Gy gcg Al a t cc Ser cca Pr o ctt Leu 230 cga Ar g t ca Ser gca Al a t cc Ser aag Lys 310 cag gca G n Al a att tct I e Ser at c gag lie G u 200 atc tgg lie Trp 215 gat cac Asp Hi s gca at c Ala I e cag gca G n Al a cag ggc G n y 280 cac cac Hi s Hi s 295 ttc gat Phe Asp ttg cac acc acc acg gcg acg Leu His Thr Thr Thr Ala Thr 170 175 tca tgg aag gca ttt gct cct Ser Trp Lys Ala Phe Ala Pro 185 190 gca gta gat cgc gca atg cgt Ala Val Asp Arg Ala IVet Ar g 205 gaa ggc gaa gac ggc gta at c Su G y u Asp y Val lie 220 atc tac acc att cct ttg cct Ile Tyr Thr Ile Pro Leu Pro 235 ttg gat acc tac acc aag gaa Leu Asp Thr Tyr Thr Lys G u 250 255 ccg atc gac ttg gcg cgc agc Pro lie Asp Leu Ala Arg Ser 265 270 ttg gac ctg cgt gat gtg gac Leu Asp Leu Arg Asp Val Asp 285 act cac tac gtg atc ggc acc Thr His Tyr Val Ile y Thr 300 cca gat gca tcg cga gaa acc Pro Asp Ala Ser Arg G u Thr 315 agg Ar g gcg Al a ggc G y gcg Al a gca Al a 240 cac Hi s at g bet t cc Ser gga G y ctt Leu 320 <210> 604 <211> 325 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 604 Asp Pro Val u Val Ala Ala Asp Thr 1 5 lie lie Asp Asn Ala Ser Val n Ala 25 Val Ser Ser Ala Arg Al a IVt Ala G n 40 Arg y Ala Ser Val Phe y Leu Pro Al a Al a Leu Al a Asn G y Thr Al a Val 70 Thr Phe Leu Ala Ala G u Tyr Ser His Gn Gu I 10 Al a Ser Val Arg I G y Arg Arg u I Pro G y Page 782 SIle Leu Val Al a Asp Asn SIle Ar g Thr Al a Phe SIle Thr Ar g Asp G u Hi s Pr o Gene Sequence 24-2-06.txt Lys G y G y Lys SIle Ar g bet Ser 145 I le G n Phe G u Tr p 225 G u Ser Gy I e Ser 305 Asp Al a SIle 115 Leu Al a G n Ar g Gy 195 Al a Leu G u G u Lys 275 Leu Asp Ser Al a Thr G u Gy I e 165 Gy bet Ser Gy Lys 245 G n Al a Thr G n 3et 325 n Gy Hi s SIle 150 Gy Al a Ser Pr o Leu 230 Ar g Ser Al a Ser Lys 310 G n Ty r Lys 135 Sy G n l Ie SIle SIle 215 Asp Al a G n G n Hi s 295 Phe Al a G u 120 I le Thr Al a Ser G u 200 Tr p Hi s l Ie Al a Sy 280 Hi s Asp Sy 105 I le Asp Leu Leu Ser 185 Al a Su I e Leu Pr o 265 Leu Thr Pr o Val Val Asp 155 Thr Lys Asp G u Thr 235 Thr Asp Leu Tyr Al a 315 Asn Al a 140 Leu Thr Al a Ar g Asp 220 I le Tyr Leu Ar g Val 300 Ser Leu 125 Hi s Asp Thr Phe Al a 205 Sy Pr o Thr Al a Asp 285 I le Ar g Asp 110 Val Leu Val Al a Al a 190 bet Val Leu Lys Ar g 270 Val Sy G u Leu Ar g Sy Asp Thr 175 Pr o Ar g SIe Pr o G u 255 Ser Asp Thr Thr <210> 605 <211> 431 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> <223> FRXA00514 <400> 605 cac gag cgt tcc tat gct cct gag cga gcc ctc cgc cga gag acc atc His G u Arg Ser Tyr Al a Pro G u Arg Ala Leu Arg Arg G u Thr Ile 1 5 10 gag ctg tgg aac aag att tcc acg gtg gag gat cct gaa tgg acc agg G u Leu Trp Asn Lys I e Ser Thr Val G u Asp Pro G u Trp Thr Arg 25 cgt tac cac tcc gtt gat cct gca gaa aag gcc ttc ggc gca cgc gca Page 783 Arg Tyr His Ser Val Asp Pro gtg atc acc ttc aag gat gga Val lie Thr Phe Lys Asp y 55 gcg aat gcg cat cct ctg gga Ala Asn Ala His Pro Leu G y 70 att cag aaa ttc cgc acc ttg Ile G n Lys Phe Arg Thr Leu cag gat cgc ttc ttg gat gcg G n Asp Arg Phe Leu Asp Ala 100 ctt tca gaa ctc aac att gaa Leu Ser G u Leu Asn II e G u 115 cct gtg att ccg gaa gga ctg Pro Val I e Pro G u G y Leu 130 135 <210> 606 <211> 136 <212> PRT <213> Corynebacter i um gl ut a <400> 606 His G u Arg Ser Tyr Ala Pro 1 5 G u Leu Trp Asn Lys I e Ser Arg Tyr His Ser Val Asp Pro Val lie Thr Phe Lys Asp y Ala Asn Ala His Pro Leu G y 70 Ile G n Lys Phe Arg Thr Leu G n Asp Arg Phe Leu Asp Ala 100 Leu Ser G u Leu Asn IIe G u 115 Pro Val IIe Pro G u G y Leu 130 135 <210> 607 <211> 718 <212> DNA <213> Corynebacter i um gl utar <220> Gene Sequence 24-2-06.txt Ala G u Lys Ala Phe y Ala Arg 40 acc gtc gtg gaa gat gaa ctg gct Thr Val Val G u Asp G u Leu Ala gca cgg cct ttc gct agg gag cag Ala Arg Pro Phe Ala Arg G u G n gct gaa ggt gtt gtg tcc gaa aag Ala G u y Val Val Ser G u Lys 90 gca cag cgt acg cac gag ctt gag Ala n Arg Thr His u Leu u 105 110 ttg gat gcc gat att ttg gcc aag Leu Asp Ala Asp Ile Leu Ala Lys 120 125 ttc tgatggcggg tttgttttcc tct Phe ri cum G u Arg Thr Val 25 Al a G u 40 Thr Val Al a Ar g Al a G u Al a G n 105 Leu Asp 120 Phe Al a 10 G u Lys Val Pr o Sy 90 Ar g Al a Leu Asp Al a G u Phe Val Thr Asp Ar g G u Sy G u Ar g Ser G u Leu 125 G u Tr p Al a Leu G u G u Leu 110 Al a Thr Thr Ar g Al a G n Lys G u Lys Page 784 Gene Sequence 24-2-06.txt <221> CDS <222> (101)..(718) <223> RXA00512 <400> 607 tacaacgagt acaacgcttt cgaccagcaa gtattcacct cccatcttct aacccgccta tatataagga gtgaatcacc attccgctga cagctacaag acc Thr gcc Al a t ac Tyr t ac Tyr ttc Phe t cc Ser cgc Ar g acc Thr ctt Leu at c SIle 150 at g Net gt c Val aac Asn gat Asp t cc Ser gt g Val ct g Leu gag G u at c SIle gcg Al a tct Ser 120 at g et gcc Al a ttt Phe gat Asp t cc Ser 200 cgc Ar g gt c Val gat Asp cac Hi s ggc G y tct Ser t cc Ser cac Hi s ct a Leu gac Asp act Thr 170 gag Gu ttc Phe aaa Lys at g bet tt g Leu ggc Gy cgt Ar g 75 tt g Leu tac Tyr at c SIle gcc Al a t cc Ser 155 ggc Gy aaa Lys acc Thr ggg Gy cca Pr o gt g Val gag Gu t cc Ser ccc Pr o at g Net cgc Ar g at g Net 140 agc Ser ccg Pr o t ca Ser gcc Al a ct c Leu gag Gu gaa Gu 45 ct g Leu t ac Tyr aaa Lys ggc G y aaa Lys 125 gat Asp aag Lys gaa Gu ct g Leu cgc Ar g 205 t ac Tyr acc Thr aac Asn ccc Pr o cgc Ar g gaa Gu acc Thr 110 gtt Val at t SIle t ca Ser t ca Ser at c SIle 190 gtg Val gga Gy 15 aat Asn tgc Cys act Thr t cc Ser gcc Al a 95 aag Lys ggc G y cgt Ar g gt c Val cct Pr o 175 gt c Val t ca Ser agc Ser gcg Al a ct g Leu 80 cac Hi s gat Asp cac Hi s cgc Ar g gcc Al a 160 gca Al a at g Net 1 at c SIle ct g Leu ttc Phe caa G n gat Asp ccg Pr o t cc Ser acc Thr cgc Ar g 145 gaa Gu t cc Ser tcc agc gcc aca Ser Ser Ala Thr gcc gat tac acg Ala Asp Tyr Thr acc tac cgt ggc Thr Tyr Arg y gag gag gtg ttt G u G u Val Phe caa ctt gcg gag G n Leu Al a G u gcc ggt ttg atc Al a G y Leu Ile atg gat gtt atg [et Asp Val bet 100 gag tat ttc acc G u Tyr Phe Thr 115 ttg ttg gcg cag Leu Leu Ala G n 130 aag ggc ctc gat Lys G y Leu Asp aac ctg ctg tct Asn Leu Leu Ser 165 aac cca gct gac Asn Pro Ala Asp 180 ctc tac gcc gag cac tcc ttc Leu Tyr Ala G u His Ser Phe <210> 608 <211> 206 <212> PRT <213> Corynebacteri um gl utami cum Page 785 Gene Sequence 24-2-06.txt <400> 608 Net 1 I e Leu Phe G n Asp Pr o Ser Thr Ar g 145 G u Ser Al a Ser Asp Tyr G u Leu Gy Asp Tyr 115 Leu Gy Leu Pr o Hi s 195 Thr 5 Thr Gy Phe G u SIle bet Thr G n Asp Ser 165 Asp Thr Al a Ty r Ty r Phe 70 Ser Ar g Thr Leu SIle 150 bet Val Thr Val Al a Leu Asn Leu Thr Asp Pr o 135 I le Val Ar g Val Lys G u Tr p Ar g Hi s Val 105 G u Val Pr o Gy Phe 185 Lys G y Leu Tyr Nbet Leu Gy Ar g 75 Leu Tyr SIle Al a Ser 155 Gy Lys G u Q u Leu Tyr Lys Gy Lys 125 Asp Lys G u Leu G y Val Asn Ser Cys Ser Thr Al a Ser Leu Al a Hi s Lys Asp G y Hi s Arg Arg Val Ala 160 Pro Al a 175 Leu Tyr Ser Phe Asn Ala Ser Thr Phe Thr Ala 200 Arg Val 205 <210> 609 <211> 320 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> <223> RXA00518 <400> 609 gtc ctt ccg gta tta gct gcc cgc cac Val Leu Pro Val Leu Ala Ala Arg His 1 5 atg tat gaa aac atg cgc gac gcc atg IVet Tyr G u Asn IVet Arg Asp Ala IVet 25 ccg aat ctc gat ttc cct gct ggc cct Pro Asn Leu Asp Phe Pro Ala Gy Pro 40 ccg gtc gat ttc ttc acc ccg ctg ttc Pro Val Asp Phe Phe Thr Pro Leu Phe 55 gac ggc gaa aag tgg gtt gcc Asp G y u Lys Trp Val Ala 10 gac gcc cgc acc ggc atc aag Asp Ala Arg Thr Gy Ile Lys gcc tac cac ctg ctc ggt ttc Ala Tyr His Leu Leu Gy Phe gtc atc gcc cgc gtc gcc ggc Val lie Ala Arg Val Ala G y Page 786 Gene Sequence 24- 2- 06. txt tgg acg gcc cac atc gtg gag cag tac gaa aac aac tcg ctc atc cgc 240 Trp Thr Ala His Ile Val G u G n Tyr G u Asn Asn Ser Leu lie Arg 70 75 cca ctg tcc gag tac aac ggc gag gag cag cgc gag gtc gcg ccc att 288 Pro Leu Ser u Tyr Asn y Gu u Gn Arg u Val Ala Pro lie 90 gaa aag cgc taaaagattt tcgcttttcg acg 320 G u Lys Arg <210> 610 <211> 99 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 610 Val Leu Pro Val Leu Ala Ala Arg His Asp Gy G u Lys Trp Val Ala 1 5 10 IVet Tyr G u Asn IVet Arg Asp Al a IVet Asp Al a Arg Thr G y I e Lys 25 Pro Asn Leu Asp Phe Pro Ala G y Pro Ala Tyr His Leu Leu G y Phe 40 Pro Val Asp Phe Phe Thr Pro Leu Phe Val Ile Ala Arg Val Ala y 55 Trp Thr Ala His Ile Val G u G n Tyr G u Asn Asn Ser Leu lie Arg 70 75 Pro Leu Ser u Tyr Asn Gy Gu Gu Gn Arg Gu Val Ala Pro lie 90 G u Lys Arg <210> 611 <211> 1494 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1471) <223> RXA01077 <400> 611 tctatgactt gatccacaat gtgatgcaaa tcattgaccc tcaccccgga ccaagcgctt aatgaaggca agccaaactt aactagtaga taggattgca atg acc gaa tcg caa 115 IVet Thr G u Ser G n 1 gat ctc gcc gca ttc gtg gaa gct gcc aaa ctc aat gat gca agc ccc 163 Asp Leu Ala Ala Phe Val G u Ala Ala Lys Leu Asn Asp Ala Ser Pro 15 gaa gcc gta gag caa ttg aaa atc aga gtg cta gac acc gta ggc gtt 211 G u Ala Val G u G n Leu Lys lie Arg Val Leu Asp Thr Val y Val 30 gcc att ggc gca ctg gat gcc gaa ccg att gtc gcc att cga gga ctc 259 Page 787 Al a I le G y Ala Leu Asp Ala Gene Sequence 24- 2- 06. txt Su Pro Ile Val Ala I e Arg y Leu ct g Leu aaa Lys t ac Tyr ccc Pr o gcc Al a cac Hi s cac Hi s 150 ct g Leu aca Thr tgg Tr p gca Al a ggc Gy 230 tgg Tr p cac Hi s at a SIle ct g Leu gac Asp agt Ser gac Asp gat Asp gga Gy 120 aga Ar g acc Thr ttg Leu aat Asn ggc G y 200 ct c Leu aag Lys aag Lys gcg Al a gca Al a 280 act Thr ggg G y gaa G u at g let ttc Phe gac Asp t ca Ser gga G y gct Al a 170 gca Al a gcc Al a agc Ser ttc Phe gac Asp 250 at t l Ie gaa G u gac Asp gga G y cgt Ar g 75 gac Asp gga Gy ct g Leu gat Asp gca Al a 155 gat Asp ctt Leu tac Ty r cga Ar g aaa Lys 235 tt g Leu cac Hi s ggc Gy gtt Val acc Thr gca Al a gcc Al a gca Al a ct c Leu gt c Val 140 ttc Phe caa G n cgt Ar g cca Pr o ggt G y 220 gag G u gaa G u tcg Ser ttc Phe gcc Al a 300 gaa G u gca Al a t ac Tyr gt g Val acc Thr 125 gca Al a gca Al a at c SIle gt c Val cac Hi s 205 at t SIle t cc Ser agc Ser cag G n aat Asn 285 t ac Tyr cag G n ttt Phe ct a Leu ct c Leu 110 gca Al a cca Pr o gcg Al a gcc Al a act Thr 190 gt g Val acc Thr gt c Val gtt Val t ca Ser 270 gt g Val t cc Ser t ca Ser ttc Phe gca Al a 95 gct Al a ttc Phe gtt Val ggc G y aac Asn 175 cgc Ar g t cc Ser ggt G y t cc Ser aag Lys 255 gcg Al a gac Asp at c SIle aca Thr aac Asn 80 aag Lys gca Al a gcc Al a cgc Ar g gca Al a 160 gca Al a act Thr aaa Lys ccg Pr o gga Gy 240 cgc Ar g ctt Leu aac Asn at t SIle ctt Leu agc Ser ggc Gy gcc Al a gt g Val gcc Al a 145 tct Ser ct g Leu gga G y gaa G u gaa G u 225 ccg Pr o acc Thr gat Asp at t SIle ggc G3y 305 at t SIle gca Al a gaa G u gaa G u gcc Al a 130 aaa Lys gct Al a gcc Al a aac Asn gga G y 210 gaa G u ttc Phe at c SIle gca Al a gaa G u 290 ggc Gy ggt G y agc Ser aac Asn 100 gtt Val cag G n ttc Phe aag Lys gca Al a 180 agc Ser tgg Tr p ttc Phe at c SIle aag Lys 260 caa G n at t SIle gaa G u ggc G y cgc Ar g cac Hi s ggc Gy gt a Val gat Asp gca Al a 165 gga G y cac Hi s gca Al a gaa G u gat Asp 245 aaa Lys gaa G u cac Hi s gaa G u 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 ggc gac aaa cag ctt att cgc acc aaa gaa gaa gcc gat cac tca ctg Page 788 Gene Sequence 24-2-06.txt G y Asp Lys G n Leu I e Arg Thr Lys G u G u Ala Asp His Ser Leu 310 315 320 325 ccg tgg atg ctc gct gta gtt ctg ctg gat ggt cag ctc aat ccc gaa 1123 Pro Trp IVet Leu Ala Val Val Leu Leu Asp y G n Leu Asn Pro G u 330 335 340 cag tac gaa cca tca cgc atc gtt gct gat gat gta caa acc ttg atg 1171 G n Tyr G u Pro Ser Arg Ile Val Ala Asp Asp Val G n Thr Leu IVet 345 350 355 aag aaa atc gaa atc aca ccg tca gat gaa ttc tct gat cgc ttc cct 1219 Lys Lys lie G u lie Thr Pro Ser Asp G u Phe Ser Asp Arg Phe Pro 360 365 370 gac cac atg cca gct gat cta gaa gtc aca cta aac gat ggc tcg gtg 1267 Asp His IVet Pro Ala Asp Leu G u Val Thr Leu Asn Asp G y Ser Val 375 380 385 ttc aaa gct tca caa gat agc tac tta ggc ttc cac gac aat ccc cta 1315 Phe Lys Ala Ser G n Asp Ser Tyr Leu G y Phe His Asp Asn Pro Leu 390 395 400 405 gat tgg gac aac gcg cgc aag aaa ttc gat gcc ctt gtc aca cca ttc 1363 Asp Trp Asp Asn Ala Arg Lys Lys Phe Asp Ala Leu Val Thr Pro Phe 410 415 420 acc ggt gaa gaa cta cgt gaa gaa atc gcc acg atc att cac gag ctc 1411 Thr G y G u G u Leu Arg u u lie Ala Thr lie lie His u Leu 425 430 435 gat agc cga cag gtt tct gaa ctc aca gaa gcc ctg gcc aaa gtc tcc 1459 Asp Ser Arg G n Val Ser G u Leu Thr G u Ala Leu Ala Lys Val Ser 440 445 450 acc acc cgc agc taaaactttt tgaaaggagc tca 1494 Thr Thr Arg Ser 455 <210> 612 <211> 457 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 612 IVet Thr u Ser G n Asp Leu Ala Ala Phe Val G u Ala Ala Lys Leu 1 5 10 Asn Asp Ala Ser Pro G u Ala Val G u G n Leu Lys lie Arg Val Leu 25 Asp Thr Val y Val Ala I Ie y Ala Leu Asp Ala u Pro Ile Val 40 Al a lie Arg G y Leu Leu G u Asp Leu G y G y Thr G u n Ser Thr 55 Leu lie y y y Lys Thr Ser Pro G u Arg Ala Ala Phe Phe Asn 70 75 Ser Ala Leu Ser Arg Tyr Leu Asp Phe IVet Asp Ala Tyr Leu Ala Lys 90 G y G u Thr Asn His Pro Ser Asp Asn Phe G y Ala Val Leu Ala Ala 100 105 110 Ala u Ser Val y Al a Ser y Lys Asp Leu Leu Thr Ala Phe Ala Page 789 Gene Sequence 24-2-06.txt 120 Arg Leu Ser 125 Ala Pro Val Arg Val Al a Tyr 130 Al a Lys G y 145 Ser Al a Al a Leu Al a li e G y Asn Leu 195 G u G y Thr 210 G u G u Val 225 Pro Phe G u Thr Ile lle Asp Al a Al a 275 Ile G u Lys 290 Sy y y 305 Al a Asp Hi s G n Leu Asn Val G n Thr 355 Ser Asp Ar g 370 Asn Asp G y 385 Hi s Asp Asn Leu Val Thr Ile Ile Hi s 435 Leu Ala Lys 450 <210> 613 <211> 923 Val Asp Al a 165 Gy Hi s Al a G u Asp 245 Lys G u Hi s G u Leu 325 G u vet Pr o Val Leu 405 Phe Leu Ser Thr 135 Thr Gy Al a Lys Leu 215 Asn Ser Asn Ar g Thr 295 Asp Tr p Tyr Lys Hi s 375 Lys Tr p Gy Ser Thr 455 Thr Leu Asn Gy 200 Leu Lys Lys Al a Al a 280 Thr Lys bet G u SIle 360 vet Al a Asp G u Ar g 440 Ar g n Pr o Val 185 Leu Al a Gy G u G u 265 G n Phe G n Leu Pr o 345 G u Pr o Ser Asn G u 425 G n Ser Gy Al a 170 Al a Al a Ser Phe Asp 250 I le G u Asp Leu Al a 330 Ser SIle Al a G n Al a 410 Leu Val Asp Val 140 Al a Phe 155 Asp n Leu Arg Tyr Pro Arg G y 220 Lys G u 235 Leu G u Hi s Ser G y Phe Val Ala 300 li e Ar g 315 Val Val Arg Ile Thr Pro Asp Leu 380 Asp Ser 395 Arg Lys Arg G u Ser G u Al a SIle Val Hi s 205 I le Ser Ser G n Asn 285 Tyr Thr Leu Val Ser 365 G u Tyr Lys G u Leu 445 Al a Al a Thr 190 Val Thr Val Val Ser 270 Val Ser Lys Leu Al a 350 Asp Val Leu Phe SIle 430 Thr Gy Asn 175 Ar g Ser Gy Ser Lys 255 Al a Asp SIle G u Asp 335 Asp G u Thr Gy Asp 415 Al a G u Al a 160 Al a Thr Lys Pr o Gy 240 Ar g Leu Asn SIe G u 320 Gy Asp Phe Leu Phe 400 Al a Thr Al a Page 790 Gene Sequence 24-2-06.txt <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> RXN03144 <400> 613 acc Thr 1 aag Lys gat Asp gaa G u cat Hi s act Thr gat Asp gaa G u gt g Val tcg Ser 145 ctt Leu t cc Ser gag G u aag Lys at t SIle 225 acg Thr ttc Phe gcg Al a ggg G y cca Pr o aca Thr gcg Al a at t SIle 115 ggc Gy gaa G u gat Asp cgc Ar g cct Pr o 195 ttt Phe gat Asp acc agg cag tcg cga aaa Thr Arg G n Ser Arg Lys 5 10 cca gcg ttt gcg gga aag Pro Ala Phe Ala Gy Lys cgt ggg gag ggt tcg ccc Arg y u Gy Ser Pro 40 atc gcg tgg ctg tta tcg lie Ala Trp Leu Leu Ser ccg gaa cac ggc gag ccc Pro u His Gy u Pro 70 gaa cat tca gcg gaa tat G u His Ser Ala G u Tyr 90 agg atg aag cca ctg gtt Arg IVet Lys Pro Leu Val 105 gag att gtg ctg cgc acc G u Ile Val Leu Arg Thr 120 ggg gcg aac gat ccg cag G y Ala Asn Asp Pro G n 135 ctg gat cat tcc atc atg Leu Asp His Ser Ile IVet 150 gtg tgg cac cac gag ttt Val Trp His His G u Phe 165 170 gaa act gtg gag ctg tgg G u Thr Val u Leu Tr p 185 tgg acg cgc cga tac cat Trp Thr Arg Arg Tyr His 200 gcg aaa gca gtg atc aca Ala Lys Ala Val lie Thr 215 ttg gct gtc gcg gat gcc Leu Ala Val Ala Asp Ala 230 ggt gag att tcc agc tgg Gy u lie Ser Ser Trp at g et gca Al a ggc G y aag Lys 75 caa G n gac Asp agt Ser aag Lys t ac Tyr 155 t cc Ser cac Hi s tct Ser at g et cac Hi s 235 gcc Al a ccg Pr o aaa Lys ct g Leu tcg Ser gcg Al a cac Hi s at g Net 140 at t SIle t ac Tyr aag Lys gat Asp gct Al a 220 ccg Pr o at t l Ie at t I e gat Asp ggg Gy cag G n gct Al a cac Hi s 125 gat Asp ttc Phe acc Thr at t SIle gat Asp 205 gat Asp ct g Leu gag G u tgg Tr p cat Hi s at t SIle gca Al a ggc G y 110 acg Thr ccg Pr o gcc Al a cgc Ar g cgc Ar g 190 cct Pr o ggc Sy ggt Gy gcg Al a gag G u gtt Val ct a Leu ccg Pr o gga G y cat Hi s cag G n gt c Val aag Lys 175 acc Thr gca Al a acc Thr gct Al a at g bet ggc G y t at Tyr gag G u att SIe acg Thr t at Tyr gcc Al a gcg Al a 160 cgt Ar g gtg Val aaa Lys gtg Val cgg Ar g 240 Page 791 ccg ttt gcg cgg gag aat tac att g Pro Phe Ala Arg G u Asn Tyr Ile C 245 ggg att gtc att gat tca gaa cag g Sy Ile Val lie Asp Ser u n C 260 2 agc ctg cct gac ctg gat gat ctt g Ser Leu Pro Asp Leu Asp Asp Leu A 275 280 ata agc aac cag gcc gcg acg aaa g Ile Ser Asn G n Ala Al a Thr Lys A 290 295 ttcgaatggt gtt <210> 614 <211> 300 <212> PRT <213> Corynebacter i um gl ut ai cum Sequence 24-2-06.txt aa aaa ttc cgc aca ctc gcg cag Su Lys Phe Arg Thr Leu Ala G n 250 255 aa cgc ttc ttg cat gcc gtg caa Su Arg Phe Leu His Ala Val G n 65 270 iat cag ctc aac atc gaa gtc gac sp n Leu Asn lie u Val Asp 285 cg ggg ctg tta tgaatctctt l a G y Leu Leu 300 <400> 614 Thr Thr Thr Al a 1 Lys Ala Phe Al a Asp Arg Ala IVet G u Asp G y Val His Val Pro Leu Thr Tyr Thr Lys Asp Leu Ala Arg 100 G u His Ile Al a 115 Val Ile y Thr 130 Ser Arg G u Thr 145 Leu G n Asp G y Ser Thr Arg Pro 180 G u Asp Pro G u 195 Lys Al a Phe G y 210 Thr 5 Pr o Ar g SIle Pr o G u Ar g G u Sy Leu Val 165 G u Tr p Al a Arg G n Ser Arg Al a Sy Al a G u 70 Hi s bet SIle Al a Asp 150 Tr p Thr Thr Lys Phe G u Tr p 55 Hi s Ser Lys Val Asn 135 Hi s Hi s Val Ar g Al a 215 Al a Sy 40 Leu Sy Al a Pr o Leu 120 Asp Ser Hi s G u Ar g 200 Val Sy 25 Ser Leu G u G u Leu 105 Ar g Pr o SIle G u Leu 185 Tyr SIle Sy bet Al a Sy Lys G n Asp Ser Lys Tyr 155 Ser Hi s Ser G u Al a Pr o Lys Leu Ser Al a Hi s bet 140 I le Tyr Lys Asp I e l e I e Asp Sy G n Al a Hi s 125 Asp Phe Thr SIle Asp 205 Ser G u Tr p Hi s SIle Al a Sy 110 Thr Pr o Al a Ar g Ar g 190 Pr o Ser Al a G u Val Leu Pr o Sy Hi s G n Val Lys 175 Thr Al a Thr bet Al a Asp G y Thr Val 220 Page 792 ene Sequence 24 Ile OuAsp Ou Leu AlaVal AlaAsp Ala His 225 230 235 Pr o Phe Ala Ar g O31u Asn Tyr Ilie O31u Lys Phe 245 250 G31y Ilie Val Ilie Asp Ser 31u O31n O31u Ar g Phe 260 265 Ser Leu Pro Asp Leu Asp Asp Leu Asp O31n Leu 275 280 Ilie Ser Asn O31n Al a Al a Thr Lys Al a O31y Leu 290 295 <210> 615 <211> 511 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221> ODS <222> (511) <223> FRXA02322 <400> 615 cgcgcagagc t aaact gcgt gaggt t gt gg cct gt cacac 2- 06. t xt Pro Leu Gy Ala Arg 240 Ar g Thr Leu Al a O31n 255 Leu Hi s Al a Val O31n 270 As n IIe O31u Val As p 285 Le u 300 at aat cggcc t agggt ggga ctttaaggaa acagtgcaca aataaatctc aaggagcccc gt t ValI gcg Al a g at As p t ct Ser gcg Al a ggc G31y cgt Ar g ccc Pr o ag c Ser ac c Thr aaa Lys5 t cg Ser gcg Al a t cc Ser t ac Tyr t tg Le u g ac As p 120 ggc (31y ct t Le u t cc Ser at g vb t gt g ValI ccg Pr o cca Pr o t tc Ph e gcc Al a ct t Le u at c I Ie cgc Ar g g aa O31u t gg Tr p g at As p g at As p gcc Al a aac As n 45 cgg Ar g aag Lys5 gct Al a ac a Thr aac As n ac c Thr cgg Ar g cct Pr a G31y gcc Al a tt t Ph e 110 tt t Ph e 15 g ac As p at c I Ie gt g ValI gga G31y tt t Ph e 95 t ta Le u ccc Pr a ccc Pr a at c I Ie act Thr aaa Lys5 gct Al a gca Al a at g vb t 1 aaa Lys5 gt g ValI g ac As p gt g ValI gt t ValI aat As n gct Al a cgc at c cac Ar g I Ie Hi s gca Al a gag O31u aac As n gcc Al a tt t Ph e ggt G31y g aa O31u c ag O31n 130 gag O31u gt g ValI gcg Al a ag g Ar g gga O31y gt g ValI t ac Tyr 115 c ac Hi s ac a Thr gcg Al a c aa O31n at t I Ie gcc Al a 1 00 t cc Ser at t cca cca ct t ct t gca gt a gcg Leu Al a Val Al a gct cag aga Ala O31n Ar g I Ie Pro Pro Le u 125 Page 793 Gene Sequence 24-2-06.txt <210> 616 <211> 137 <212> PRT <213> Corynebact eri um gl ut arri cum <400> 616 IVet Arg lie His Asp Val Tyr Thr His 1 5 Lys Ala u His Leu Ala Trp Lys Phe 25 Val G u Val Thr Pro Asp Val Ser G u 40 Asp Asn Ala Ala Val Ser Ala Ala Ser 55 Val Ala Arg G n n Al a n Ser His 70 Val Phe Gy Ile Ser G y Ser Tyr Ser Asn y Val Ala Val Arg G u Leu Asp 100 105 Ala G u Tyr Ser His Pro G y Asp Asn 115 120 Ala G n Ala G n Arg Ser Ser y Arg 130 135 <210> 617 <211> 603 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> <223> RXA02329 <400> 617 acg gct acc agg cag tcg cga aaa ggt Thr Ala Thr Arg G n Ser Arg Lys y 1 5 ttc gcg cca gcg ttt gcg gga aag atg Phe Al a Pro Al a Phe Al a G y Lys IVet 25 gcg atg cgt ggg gag ggt tcg ccc gca Ala IVet Arg Gy u y Ser Pro Ala 40 ggg gtc atc gcg tgg ctg tta tcg ggc Sy Val Ile Ala Trp Leu Leu Ser y 55 cca ttg ccg gaa cac ggc gag ccc aag Pro Leu Pro G u His G y G u Pro Lys 70 aca aag gaa cat tca gcg gaa tat caa Ser G u SIle Leu Ar g G u Hi s Pr o Al a Leu SIle Ar g G u Tr p Asp Pr o Asp Al a Asn Ar g Lys Al a Thr Leu 125 agc Ser gcg Al a gag G u gtt Val ct a Leu ccg Asn Thr Ar g Pr o Gy Al a Phe 110 Leu tgg Tr p at g Net ggc Gy t at Tyr gag Gtu at t Phe Asp SIe Val Gy Phe Leu Al a aag Lys gat Asp gaa G u cat Hi s act Thr gat gag att tcc G u i e Ser 10 gcc att gag Al a ll e G u ccg att tgg Pro II e Tr p aaa gat cat Lys Asp Hi s ctg ggg att Leu G y II e tcg cag gca Page 794 Gene Sequence 24-2-06.txt Thr Lys G u His Ser Al a G u Tyr G n Ser n Ala Pro Ile Asp Leu 90 gcg cgc agg atg aag cca ctg gtt gac gcg gct ggc gga acg gaa cac 336 Ala Arg Arg IVet Lys Pro Leu Val Asp Ala Ala G y G y Thr u Hi s 100 105 110 att gca gag att gtg ctg cgc acc agt cac cac acg cat tat gtg att 384 Ile Ala u Ile Val Leu Arg Thr Ser His His Thr His Tyr Val lle 115 120 125 ggc act ggg gcg aac gat ccg cag aag atg gat ccg cag gcc tcg cgt 432 G y Thr G y Al a Asn Asp Pro G n Lys IVet Asp Pro G n Al a Ser Arg 130 135 140 gaa acc ctg gat cat tcc atc atg tac att ttc gcc gtc gcg ctt caa 480 G u Thr Leu Asp His Ser Ile IVet Tyr Ile Phe Ala Val Ala Leu G n 145 150 155 160 gat ggc gtg tgg cac cac gag ttt tcc tac acc cgc aag cgt tcc acc 528 Asp G y Val Trp His His G u Phe Ser Tyr Thr Arg Lys Arg Ser Thr 165 170 175 cgc ccg gaa act gtg gag ctg tgg cac aag att cgc acc gtg gag gat 576 Arg Pro G u Thr Val G u Leu Trp His Lys Ile Arg Thr Val G u Asp 180 185 190 cct gaa tgg acg cgc cga tac cat tct 603 Pro G u Trp Thr Arg Arg Tyr His Ser 195 200 <210> 618 <211> 201 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 618 Thr Ala Thr Arg n Ser Arg Lys Oy u lie Ser Ser Trp Lys Ala 1 5 10 Phe Al a Pro Al a Phe Al a G y Lys IVet Al a I e u Al a Iet Asp Arg 25 Ala IVet Arg y u y Ser Pro Ala Pro Ile Trp u y u Asp 40 G y Val IIe Ala Trp Leu Leu Ser G y Lys Asp His Val Tyr His Val 55 Pro Leu Pro G u His G y G u Pro Lys Leu G y IIe Leu G u Thr Tyr 70 75 Thr Lys G u His Ser Al a G u Tyr G n Ser G n Ala Pro lie Asp Leu 90 Al a Arg Arg IVet Lys Pro Leu Val Asp Al a Al a G y G y Thr G u Hi s 100 105 110 Ile Ala u Ile Val Leu Arg Thr Ser His His Thr His Tyr Val lle 115 120 125 G y Thr G y Al a Asn Asp Pro G n Lys IVet Asp Pro G n Al a Ser Arg 130 135 140 G u Thr Leu Asp His Ser Ile IVet Tyr Ile Phe Ala Val Ala Leu G n 145 150 155 160 Page 795 Gene Sequence 24-2-06.txt Asp G y Val Trp His His G u Phe Ser Tyr Thr Arg Lys Arg Ser Thr 165 170 175 Arg Pro G u Thr Val G u Leu Trp His Lys lie Arg Thr Val G u Asp 180 185 190 Pro G u Trp Thr Arg Arg Tyr His Ser 195 200 <210> 619 <211> 1266 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1243) <223> RXA02332 <400> 619 gaccgcatgc aacaccgcag cagattatat gagctcctgc ga tttgaccagc acattttcac ctacagaaaa ggagaaaaca at t acgaaga ctacaacgtc g agt gt c Val aaa Lys gag G u tgg Tr p aca Thr cac Hi s gt g Val gat Asp gt g Val cct Pr o 150 aaa Lys at g Met ttg Leu ggg G y cga Ar g tta Leu t ac Tyr at c SIle 120 gcc Al a cac Hi s ct c Leu gag G u gaa Gu ct g Leu t ac Tyr aac Asn ggt Gy agc Ser gat Asp aaa Lys ggc G y aac Asn tgc Cys acc Thr t ca Ser tgc Cys ttt Phe gga Gy cgc Ar g 140 at c SIle gt c Val tcg Ser agc Ser 45 act Thr ct c Leu cac Hi s gat Asp cac Hi s 125 agg Ar g gct Al a at c SIle ct g Leu ttt Phe gaa Gu gac Asp ccc Pr o ccc Pr o 110 aac Asn cga Ar g tcg Ser tct Ser 15 act Thr gaa Gu caa G n gcc Al a at g Net 95 gat Asp ct g Leu agt Ser aat Asn gac Asp t ac Tyr gaa Gu ct c Leu gga G y 80 gac Asp ccg Pr o ctt Leu ggg Gy ttc Phe 160 bet Ser 1 tac aca Tyr Thr cgt ggc Arg G y gtg at c Val lile cgg acc Arg Thr ctg att Leu II e gtg ct g Val Leu ttt acc Phe Thr gcg cag Al a G n 130 gaa gag Gu Gu 145 tta tcc Leu Ser agc att Ser Ile tac gcc Tyr Al a tac ct c Tyr Leu ttc aac Phe Asn t cc ct c Ser Leu cgc acc Arg Thr 100 cgc gat Arg Asp 115 ctt ccc Leu Pro at c at c Ile Ile at g gt g let Val gac agc caa Asp Ser G n t cc Ser gtg Val ctg Leu acg Thr at c SIe gca Al a gcc Al a at g let gca Al a ttt Phe 165 ggc aat gat gat ggt tct gta gcc aac tcc gca gat gac atc cgc gat Page 796 3y Asn Asp Asp G y Ser Val 170 Gene Sequence 24-2-06.txt Ala Asn Ser Ala Asp Asp I 175 le Arg Asp 180 ttt Phe aca Thr gcg Al a gcc Al a 230 aat Asn at g Net t cc Ser aaa Lys act Thr 310 at g et cga Ar g gcg Al a gt g Val cgc Ar g t ca Ser 200 aca Thr gag G u gcc Al a ttc Phe gag G u 280 gt g Val at t SIle gga G y t ca Ser at c l Ie 360 ccc Pr o t cc ctc at c Ser Leu II e 185 gcc cgc gt g Al a Ar g Val ggt gcg at c G y Al a lle ttt gtc atg Phe Val Nbet 235 gac tgg at g Asp Trp IVet 250 ggg cac cgc G y His Arg 265 aaa t cc at g Lys Ser IVet cac at g t at Hi s Net Tyr aaa ccc aac Lys Pro Asn 315 ttc ccc gtc Phe Pro Val 330 ggg tgg acg G y Tr p Thr 345 cga cca t t g Arg Pro Leu att tcg gag Ile Ser G u ct c Leu at c SIle ggt Gy 220 cac Hi s ggc Gy gt g Val cgc Ar g gag G u 300 ct c Leu gac Asp gca Al a tct Ser t ac Tyr t ca Ser 205 gct Al a acc Thr aag Lys t ac Tyr t cc Ser 285 tcg Ser gac Asp ttc Phe cac Hi s gcc Al a 365 gcc Al a 190 t ca Ser ct c Leu at g Net gcg Al a aaa Lys 270 ctt Leu at g bet ttc Phe ttc Phe at c SIle 350 t ac Tyr gag G u acg Thr aaa Lys ct g Leu ttg Leu 255 aac Asn gct Al a caa G n ccg Pr o aca Thr 335 gt g Val aac Asn cac Hi s cga Ar g ggc Gy gat Asp 240 gat Asp ggc Gy gct Al a gaa Gu gcc Al a 320 cca Pr o gag G u gga Gy t cc Ser t cc Ser cca Pr o 225 at c SIle cgt Ar g gac Asp cgt Ar g gt c Val 305 ggc G y ct g Leu caa G n gt g Val ttc Phe gat Asp 210 ct g Leu gac Asp aaa Lys t cc Ser cac Hi s 290 at g Net cct Pr o ttt Phe ttt Phe gaa 30u 370 aac Asn 195 acg Thr cac Hi s gat Asp gaa G u agg Ar g 275 cgt Ar g gag G u gcc Al a gt g Val gaa G u 355 gaa G u gcc Al a t at Tyr gga Gy ccc Pr o cgc Ar g 260 gt c Val ggt G y gct Al a t at Tyr ct g Leu 340 aac Asn agg Ar g 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1266 aga acc Arg Thr 380 taatcagtga ggctgatttc taa <210> 620 <211> 381 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 620 IVet Ser Asp Ser G n Val Arg Lys y Leu Asn y Val lie Ser Asp 1 5 10 Tyr Thr Ser I e Ser Lys Val IVet Pro G u Ser Asn Ser Leu Thr Tyr 25 Page 797 Ar g G y Tyr Ala Val u Asp Gene Sequence 24- 2- 06. txt Leu Val G u Asn Cys Ser Phe G u G u 40 Val Ar g Leu Val Phe Al a G u 145 Leu Asp Ser Ser Pr o 225 I le Ar g Asp Ar g Val 305 Gy Leu G n Val Tyr Phe Ser Ar g Ar g 115 Leu SIle let SIle Asn 195 Thr Hi s Asp G u Ar g 275 Ar g G u Al a Val G u 355 G u Leu Thr SIle Al a Al a let Al a Phe 165 Asp Ser Ser Gy Asn 245 I le Pr o G n Ar g Tyr 325 Al a Asn G u Tr p Thr 70 Hi s Val Asp Val Pr o 150 Gy Phe Thr Al a Al a 230 Asn bet Ser Lys Thr 310 bet Ar g Al a Val Phe Gy Ser Ser Hi s Val 135 Asp Asn G u Phe l Ie 215 Asn Al a Gy et Tr p 295 Gy Leu Val Leu Val 375 G u Ser Pr o bet 105 Ar g vet Asn Asp Ser 185 Al a Gy Phe Asp Gy 265 Lys Hi s Lys Phe Gy 345 Ar g SIle Pr o Ar g 75 Thr Thr SIle SI e Gy 155 Ser SIle Val SIle et 235 let Ar g et Tyr Asn 315 Val Thr Leu G u Thr Ser Cys Phe Gy Ar g 140 I le Val Leu SIle Gy 220 Hi s Gy Val Ar g G u 300 Leu Asp Al a Ser Ar g 380 Thr Leu Hi s Asp Hi s 125 Ar g Al a Al a Tyr Ser 205 Al a Thr Lys Tyr Ser 285 Ser Asp Phe Hi s Al a 365 Thr G n Al a et Asp Leu Ser Asn Ser 175 G u Thr Lys Leu Leu 255 Asn Al a G n Pr o Thr 335 Val Asn Leu Gy Asp Pr o Leu Gy Phe 160 Al a Hi s Ar g Gy Asp 240 Asp Gy Al a G u Al a 320 Pr o G u Gy <210> 621 Page 798 Gene Sequence 24-2-06.txt <211> 1038 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1015) <223> RXN02333 <400> 621 t ct t gcatgc cgtgcaaagc ctgcctgacc tggatgatct tgatcagctc aacatcgaag tcgacataag caaccaggcc gcgacgaaag cggggctgtt aat Asn gcc Al a gcg Al a gcc Al a ct g Leu ct a Leu gcc Al a cac Hi s aaa Lys gt c Val 150 gcc Al a t ac Tyr gaa G u gtt Val ccc Pr o t cc Ser at a l Ie gaa G u gt g Val cgc Ar g gaa G u 120 gt c Val gcc Al a gga Gy gat Asp gac Asp 200 gt g Val at c SIle gaa Gu gct Al a gcc Al a gt c Val gt c Val caa G n cgc Ar g cgc Ar g gaa G u 170 ggc Gy cga Ar g ggg Gy gcc Al a gaa Gu gac Asp cac Hi s 75 gac Asp gcc Al a gt c Val aca Thr gac Asp 155 gga Gy gcc Al a tac Ty r agg Ar g cgg Ar g gcc Al a ct g Leu cgc Ar g gcc Al a gaa G u aac Asn gac Asp 140 ccg Pr o at c SIle gac Asp ttc Phe cgt Ar g ct g Leu ggc Gy 45 gca Al a gcg Al a gac Asp ttg Leu ccc Pr o 125 gt g Val aac Asn gac Asp at g let cgg Ar g 205 cga Ar g ccc Pr o ttc Phe ct a Leu cgg Ar g acc Thr gag G u 110 aaa Lys at g bet ttt Phe gcc Al a at t SI e 190 cac Hi s caa G n 15 ggc Gy gaa Gu ccc Pr o caa G n ggc Gy 95 gac Asp cgt Ar g gtt Val gt c Val gcc Al a 175 ttc Phe gcc Al a gca Al a gca Al a ggc Gy gat Asp at t SIle 80 ttt Phe gcc Al a tgc Cys cga Ar g at c SIle 160 at t l Ie acc Thr at c l Ie at g aat IVet Asn 1 ttt aaa Phe Lys ttc tcc Phe Ser gtc tac Val Tyr atc ggc I e G y gcg cgc Al a Ar g ggc gaa G y G u ggt gtg G y Val ggg cac G y Hi s 130 cgc at c Arg Ile 145 tgc gcc Cys Ala gag cgc G u Arg gaa gcc G u Al a cct gat Pro Asp 210 gcg Al a cct Pr o gtt Val ttg Leu gt c Val ccc Pr o gcc Al a 115 ttg Leu gca Al a cgc Ar g gcg Al a ct c Leu 195 gcc Al a ctc ttt tcg Leu Phe Ser gca Al a ct g Leu t cc Ser acg Thr aca Thr at g et 100 gga Gy gac Asp gcc Al a acc Thr aaa Lys 180 cac Hi s ttg Leu ct c Leu att SIe ggc Gy acg Thr gac Asp tcg Ser tgc Cys ggc Gy gcc Al a gac Asp 165 gcc Al a agc Ser ttg Leu Page 799 ctg gcg aat atg Leu Al a Asn IVet 215 gtg ttg gaa gag Val Leu G u G u 230 ctg cgt att gcc Leu Arg Ile Ala gaa cac ggt acc G u Hi s G y Thr 265 aga tta tat gag Arg Leu Tyr G u 280 cac att ttc acc His Ile Phe Thr 295 gtccgcaaag gac <210> 622 <211> 305 <212> PRT <213> Corynebact <400> 622 IVet Asn Leu Phe 1 Phe Lys Ala Ala Phe Ser Pro Leu Val Tyr Val Ser I e G y Leu Thr Ala Arg Val Thr Gy u Pro Met 100 Sy Val Ala y 115 G y Hi s Leu Asp 130 Arg Ile Ala Ala 145 Cys Ala Arg Thr G u Arg Ala Lys 180 acc Thr at t SIle at g Met 250 caa G n ct c Leu t ac Tyr gaa G u ggc Gy 235 gga G y gaa G u ct g Leu aga Ar g ttt Phe 220 t ac Ty r caa G n gga G y cga Ar g aaa Lys 300 Gene Sequence 24-2-06.txt ggc aaa acg acg ctg ctg tca gcc G y Lys Thr Thr Leu Leu Ser Ala 225 aac gcc gtg atc tac ccc gtg acc Asn Al a Val Ile Tyr Pro Val Thr 240 gta gaa caa gca cta gcc gaa atc Val u G n Ala Leu Ala u lie 255 260 tgg ctg gac cgc atg caa cac cgc Trp Leu Asp Arg Iet G n His Arg 270 275 tac gaa gac tac aac gtc ttt gac Tyr G u Asp Tyr Asn Val Phe Asp 285 290 gga gaa aac aat gag tgacagccaa Sy G u Asn Asn u 305 gac Asp acg Thr 245 aaa Lys agc Ser cag G n 1025 1038 er i um gl ut ai cum Ser Asn G y Val 5 Leu SIle Sy Thr Asp Ser Cys Sy Al a Asp 165 Al a Al a Al a Al a Leu 70 Leu Al a Hi s Lys Val 150 Al a Tyr Al a Ar g Val 55 Thr Sy Al a Leu G u 135 Ser Al a Leu Pr o Ser 40 I le G u Val Ar g G u 120 Val Al a Sy Asp Asp Val 10 His Il e 25 Ile G u Al a Al a Val Ala Leu Val 90 Thr Val 105 Asp n Val Arg Arg Arg Val G u 170 Al a G y 185 Sy Al a G u Asp Hi s 75 Asp Al a Val Thr Asp 155 Sy Ar g Ar g Al a Leu Ar g Al a G u Asn Asp 140 Pr o SIle Ar g Leu Sy Al a Al a Asp Leu Pr o 125 Val Asn Asp Ar g Pr o Phe Leu Ar g Thr G u 110 Lys bet Phe Al a G n Sy G u Pr o G n Sy Asp Ar g Val Val Al a 175 Al a Al a Sy Asp SIe Phe Al a Cys Ar g SIe 160 l e Ala Asp Iet Ile Phe Thr 190 Page 800 Gene Sequence 24- 2- 06. txt Su Ala Leu His Ser u Ala Asp Phe Arg Tyr Phe Arg His Ala lie 195 200 205 Pro Asp Ala Leu Leu Leu Ala Asn IVet Thr G u Phe y Lys Thr Thr 210 215 220 Leu Leu Ser Ala Asp Val Leu G u G u lie y Tyr Asn Ala Val lie 225 230 235 240 Tyr Pro Val Thr Thr Leu Arg Ile Al a Net G y G n Val G u G n Al a 245 250 255 Leu Al a G u lie Lys G u His G y Thr Gn G u G y Trp Leu Asp Arg 260 265 270 IVet G n His Arg Ser Arg Leu Tyr G u Leu Leu Arg Tyr G u Asp Tyr 275 280 285 Asn Val Phe Asp G n His lie Phe Thr Tyr Arg Lys G y G u Asn Asn 290 295 300 G u 305 <210> 623 <211> 242 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> FRXA02333 <400> 623 gag att ggc tac aac gcc gtg atc tac ccc gtg acc acg ctg cgt att 48 Su lie y Tyr Asn Ala Val lie Tyr Pro Val Thr Thr Leu Arg lie 1 5 10 gcc atg gga caa gta gaa caa gca cta gcc gaa atc aaa gaa cac ggt 96 Al a Iet G y G n Val G u G n Ala Leu Al a u lie Lys G u Hi s y 25 acc caa gaa gga tgg ctg gac cgc atg caa cac cgc agc aga tta tat 144 Thr n G u G y Trp Leu Asp Arg Iet G n His Arg Ser Arg Leu Tyr 40 gag ctc ctg cga tac gaa gac tac aac gtc ttt gac cag cac att ttc 192 G u Leu Leu Arg Tyr G u Asp Tyr Asn Val Phe Asp G n His lie Phe 55 acc tac aga aaa gga gaa aac aat gag tgacagccaa gtccgcaaag 239 Thr Tyr Arg Lys y G u Asn Asn G u gac 242 <210> 624 <211> 73 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 624 G u lie y Tyr Asn Ala Val lie Tyr Pro Val Thr Thr Leu Arg lie 1 5 10 Page 801 ene Sequence 24- 2- 06. txt Aal'bt Gy OnVal Ou OnAla LeuAla(3u Ile Lys Ou His Oy 25 Thr O31n O31u 31y Trp Leu Asp Arg I'bt On His Arg Ser Arg Leu Tyr 40 O31u Leu Leu Ar g Tyr Ou Asp Tyr Asn Val Phe Asp On His Ilie Phe 55 Thr Tyr Ar g Lys O31y O31u As n Asn O31u <210> 625 <211> 513 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221> CDS <222> (490) <223> RXA00030 <400> 625 t aaaaacgcc act t t ggt gt cgat aacacc gaact t ccga acat aaaaga t gcgt t ggt g ct ct ct gcac aat gt t t ct t t cggaact at t ct ggcgacc at g cga at t gaa at c I'bt Arg lie Ou Ilie ac a Th r ac g Th r cgc Ar g tt g Le u at t I Ie c ag O31n g aa O31u gt a ValI gt a ValI ct c Le u tt g Le u g aa O31u cga Ar g g aa O31u ac c Th r aac As n gt t ValI tt t Ph e gt t ValI aac As n ggt O31y g ac As p gt g ValI g ac As p ct c Le u g at As p c ac Hi s CCC Pr o ct c Le u cct Pr o c ag O31n aaa Lys5 cca Pr o 45 cca Pr a gct A a aag Lys5 t ca Ser gcc A a c ac Hi s g aa O31u g at As p ac a Th r g at As p gt g ValI 110 aaa Lys5 15 g at As p aac As n gca A a c ag O31n aaa Lys5 95 at t I Ie gca A a gt a ValI cca Pr a gcg A a tt t Ph e ggc G31y gcc A a g at As p gct A a ggt G31y t ac Ty r gt t ValI g at As p ct c Le u t tc t ac ac, Phe Tyr Thi ggt gac t a, O3 y Asp Tyi gt g cag cti Val O31n Lei caa gct gg O3 n A a 03 gat gat gt Asp Asp Va t tc at c at 1 Phe Il e [Vb i 1 00 gat gac ac, Asp Asp Thi 115 t aaccccgt g at t cag at t gt t caa t tg aag cag aac IlieOn IlieVal On Leu Lys On Asn 120 gaat gacaaa at c <210O> 626 <21 1> 130 Page 802 Gene Sequence 24 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 626 Met Arg Ile u Ile Thr Ser Val Phe Val Asp 1 5 10 Leu Asp Phe Tyr Thr Thr Lys Leu G y Phe G u 25 Thr Ala y Asp Tyr Arg Trp Leu Thr Val Val 40 Asp y Val G n Leu Leu Leu G u Pro Asn G n 55 Thr Tyr n Ala y lie Lys Arg Asp y Ile 70 75 Tyr Val Asp Asp Val n G u G u Tyr Asp Ser 90 Val Asp Phe Ile Vet G u Pro Thr Asp Val y 100 105 Ile Leu Asp Asp Thr Val G y Asn Leu I e G n 115 120 Sn Asn 130 <210> 627 <211> 2334 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(2311) <223> RXN00148 <400> 627 ctttgagggc agcgcgcatg cgcccgatgg ttatttgaac gacgctggct gacctgctag atgctttggg agct t aaat c -2-06. txt Asp Gn Leu Lys I Asp Pro Hi s Pr o Pro Al a Leu Lys Pro Ser 1 Ile Val C 125 a -i s 3 u \sp Thr \sp /al 3 n Lys Asp Asn Al a G n Lys I e Leu Al a Val Pr o Al a Phe Sy Al a Lys aat Asn cac Hi s gt c Val gga G y ccg Pr o t ca Ser gtt Val cgc Ar g ccg Pr o cca Pr o at c I e gcc Al a ttc Phe gat Asp at g Net cca Pr o ggc G y acg Thr tct Ser tac Tyr 75 ttg Leu aag Lys cag G n ttg Leu acc Thr act Thr gt g Val gct Al a 45 cca Pr o aat Asn gct Al a tgg Tr p gac Asp ggt G y cag G n gag aca G u Thr 15 aac act Asn Thr cgc gac Arg Asp caa aag G n Lys ccg tgg Pro Trp 80 Page 803 atgacaattg atgccgcggc atg acg tcg atc cct IVet Thr Ser Ile Pro 1 cgt gca t cg gag tca Arg Ala Ser u Ser ccc gaa ggc att gat Pro G u G y lie Asp gag gcg caa gcg gcg G u Ala G n Ala Ala cca ttt atg cgc ggg Pro Phe IVet Arg y acg att cgc cag tac Thr lie Arg G n Tyr Gene Sequence 24-2-06.txt gca Al a ctt Leu cac Hi s at g Met gat Asp 150 gct Al a ggt Gy aaa Lys at g Met cgt Ar g 230 gcg Al a t ac Tyr cct Pr o at c SIle aaa Lys 310 cag G n gcc Al a ttt Phe gcg Al a ggt Sy 120 ggc G y at t SIle ct g Leu ggt Gy ttt Phe 200 at c SIle aac Asn gcc Al a cgt Ar g ct g Leu 280 aag Lys gat Asp tct Ser acc Thr acc Thr caa G n gac Asp gcg Al a ttg Leu at t SIle 170 gag G u gt g Val t cc Ser at t SIle ttg Leu 250 ggt Sy ttc Phe cgt Ar g aaa Lys tgg Tr p 330 at t SIle gcc Al a aaa Lys tcg Ser at t l Ie t cc Ser 155 ctt Leu cag G n cgc Ar g aac Asn tcg Ser 235 gag G u aaa Lys tt c Phe gcg Al a aac Asn 315 tcg Ser gag G u gcg gaa t cc Al a G u Ser ggt ttg tcg G y Leu Ser 110 gat aat gag Asp Asn G u 125 gat tcg att Asp Ser Ile 140 agc gt g t cg Ser Val Ser gcg ttc tat Al a Phe Tyr ctt gcg ggc Leu Al a G y 190 aac acc tat Asn Thr Tyr 205 atc ttt gag Ile Phe G u 220 att tct ggc I e Ser G y ct g gcc tac Leu Al a Tyr gag gta ggc Su Val y 270 tgg ggt att Trp G y lle 285 gga cga ctg G y Ar g Leu 300 gcc aag t cc Ala Lys Ser ttg acc gcg Leu Thr Al a gcg atg gct Al a Net Al a 350 aat gcg ttt Asn Al a Phe tat cgg agg aac gcg Al a gt g Val gat Asp tcg Ser 160 gt g Val at c SIle t at Tyr acc Thr cac Hi s 240 ct g Leu gac Asp at g et tgg Tr p tcg Ser 320 gat Asp acc Thr gat Asp ggc G y 130 cgt Ar g acc Thr gct Al a aat Asn ccg Pr o 210 ttg Leu cag G n gat Asp gat Asp acc Thr 290 gag G u cgc Ar g t ac Tyr ggc Gy Arg Arg 100 cta gcg Leu Al a 115 gat gtg Asp Val cag ctg G n Leu at g aat IVet Asn gag gaa G u G u 180 gac at c Asp Il e 195 aag ccg Lys Pr o aag at g Lys bet gaa gcg G u Al a ggt att G y lle 260 aag ttc Lys Phe 275 ttc atg Phe bet ttg gtg Leu Val acg cac Thr His aac aac Asn Asn 340 cac acc Hi s Thr 355 Asn acc Thr ggt G y ttt Phe ggc G y 165 caa G n ttg Leu tcg Ser cca Pr o gga Gy 245 gaa G u gcg Al a gag G u gca Al a tcg Ser 325 gt c Val cag G n 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 Page 804 Gene Sequence 24-2-06.txt tcg Ser ttc Phe tct Ser 390 gag G u gag G u cct Pr o gat Asp gaa G u 470 gca Al a gcg Al a gag G u gt c Val ttg Leu 550 ggc G y gaa G u cgc Ar g gga G y cac Hi s 360 gct Al a acg Thr ttg Leu gag G u ct g Leu 440 ggc Gy gag G u cag G n gt c Val aaa Lys 520 gct Al a gtt Val t ac Tyr gcg Al a cgt Ar g 600 aag Lys aat Asn at c l Ie cgt Ar g aat Asn 410 gcc Al a at t SIle cag G n at t SIle gct Al a 490 gct Al a cca Pr o cgc Ar g ttt Phe gat Asp 570 gcc Al a ttt Phe gt c Val gca ctt Al a Leu gcc cga Al a Ar g 380 cca gtt Pro Val 395 gag ctg G u Leu ggc gga G y G y gag gaa G u G u gcg ctg Al a Leu 460 gaa gt c G u Val 475 aaa ct c Lys Leu gcg ctg Al a Leu ggg gat Sy Asp gca aaa Al a Lys 540 ggc cgc G y Arg 555 gag gtt G u Val ctg gct Leu Al a att gcc I e Al a gcg tct Al a Ser 620 gat gag gcg ttg gcg Asp G u Ala Leu Al a 365 aac acc cag ctg ttg Asn Thr G n Leu Leu 385 gat cca tgg gcg ggc Asp Pro Trp Ala y 400 gct aac cgc gcg cgc Ala Asn Arg Ala Arg 415 atg gcg cag gcc acc Met Ala G n Ala Thr 430 tca gcg gca cgc acc Ser Ala Ala Arg Thr 445 atc ggc gtg aat cgc Ile y Val Asn Arg 465 ctc aag gtt gac aac Leu Lys Val Asp Asn 480 gcg caa ctg aaa gca Ala G n Leu Lys Al a 495 gat gcg ttg aca gct Asp Ala Leu Thr Ala 510 ttg gat cag aac ctg Leu Asp G n Asn Leu 525 gct acc att gga gag Ala Thr Ile Gy u 545 cac gaa gca gaa at c His G u Ala G u Ile 560 gga aag gaa ggc aca G y Lys G u G y Thr 575 gac gcc ttt gag gct Asp Al a Phe G u Al a 590 aag atg ggc cag gat Lys Iet G y G n Asp 605 gcc tat gct gac ct g Al a Tyr Al a Asp Leu ctg ccc acc gat Leu Pro Thr Asp 370 ctg cag cag gaa Leu G n 0 n G u tcc tat tac gtg Ser Tyr Tyr Val 405 aag cac atc gat Lys His Ile Asp 420 gcg cag gga att Al a n G y lle 435 cag gct cgc att Sn Ala Arg lle 450 tac gtg gcg gaa Tyr Val Ala G u acc aag gtt cgc Thr Lys Val Arg 485 gag cgc aac gat G u Arg Asn Asp 500 gct gcc cgc aac Ala Ala Arg Asn 515 ctc aaa ctt gcc Leu Lys Leu Ala 530 atc tcc gat gct Ile Ser Asp Ala agg acg ctg tct Arg Thr Leu Ser 565 gtg agc aac gtc Val Ser Asn Val 580 gag gaa ggc cgc G u G u G y Arg 595 gga cat gac cgt Gy His Asp Arg 610 ggc atg gac gtg 3 y IVet Asp Val 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 Page 805 gat gtt gga ccg ctg ttt caa Asp Val y Pro Leu Phe G n 630 635 gtg gac gcc gat gtt cac gtg Val Asp Ala Asp Val His Val 650 cac ctc acc ttg ctg ccc gag His Leu Thr Leu Leu Pro G u 665 cgc gat gac att ctg gtc acc Arg Asp Asp Ile Leu Val Thr 680 ttc cag gat ctc tac gat atg Phe G n Asp Leu Tyr Asp Iet 695 700 acc gtc atc gcg gag tcg gcg Thr Val Ile Ala u Ser Ala 710 715 cac ctg ggc ttt gac ctg gat Hi s Leu G y Phe Asp Leu Asp 730 ttcctagaag aca <210> 628 <211> 737 <212> PRT <213> Corynebacter i um gl utar Gene Sequence 24-2-06.txt act cca gcc gaa gct gcc cgc gcc Thr Pro Ala G u Ala Ala Arg Ala 640 gtg ggt atg tct tcg ctg gca gca Val Gy Vet Ser Ser Leu Ala Ala 655 660 ctg aag aaa gaa ctt gca gct ctt Leu Lys Lys G u Leu Ala Ala Leu 670 675 gtg ggc ggc gtc att ccg ccg ggc Val Gy Gy Val Ile Pro Pro y 685 690 ggt gcc gcc gcg att tac cct tca Gy Ala Ala Ala lle Tyr Pro Ser 705 atc gat ctg atc acc cga ctc gcc lle Asp Leu Ile Thr Arg Leu Ala 720 gtg gat gtg aat gaa tgatcacggt Val Asp Val Asn G u 735 gcc Al a 645 ggc G y ggc G y gat Asp gga G y gca Al a 725 2035 2083 2131 2179 2227 2275 2321 2334 <400> 628 Vet Thr Ser lie Pro Asn Phe Ser Asp lie Pro Leu Thr Ala u Thr 1 Ar g Pr o G u Pr o Thr Phe Phe Val Net 145 Ser Gy G n et Ar g Ar g Leu 115 Asp G n 5 Ser Asp Al a Gy Tyr Asn Thr Gy Phe Asn Lys Hi s Tyr Gy Al a Ar g Al a 135 Gy Asp Val Val Thr Ser Gy 105 Tyr Val Asp 10 Al a y I Phe Thr Asp Ser I Vet Tyr Thr Ala 90 G n Lys Asp Ser Al a I le Leu Ser 155 Page 806 Val Al a Pr o Asn G u Leu Asn 125 Ser Val Asn Ar g G n Pr o Asn Val Ar g Leu Val I'bt Thr I'bt Asn 03 y Al a Val1 1 65 ene Sequence 24- 2- 06. t xt Leu Pro Ilie Leu Ala Phe Tyr lieVal 170 175 Al a O31n Pr o Ser 225 li1e Al a ValI Tyr Ser 305 Le u ValI O31n Al a Le u 385 O31y Ar g Thr Thr Ar g 465 As n Al a Al a O31u As p 1 95 Ly s Ly s O31u O31y Ly s 275 Ph e Le u Thr As n Hi s 355 Pr o O31n Tyr Hi s O31n 435 Al a ValI Ly s Ar g Al a 515 O31n Le u Ser Pr o O31y 245 O31u Al a O31u Al a Ser 325 ValI O31n As p O31u ValI 405 As p I Ie I Ie O31u Ar g 485 As p As n O31y Ly s vb t Ar g 230 Al a Tyr Pr a I Ie Ly s 310 O31n Al a Ser Ph e Ser 390 O31u O31u Pr a As p O31u 470 Al a Al a (31u ValI O31u Ar g 215 Ph e Thr I Ie Ar g Al a 295 Ph e Thr Ar g Le u Ser 375 O31y Tr p ValI Ly s Ser 455 As p O31u O31u Hi s Pr a 1 85 vb t I Ie Ser As p Al a 265 Ser Le u Pr a O31y Al a 345 Thr Ar g ValI Thr O31u 425 Ar g Ar g O31u Le u Ly s 505 (31u O31u ValI Ser I Ie Le u 250 O31y Ph e Ar g Ly s Tr p 330 li1e As n I Ie Ar g As n 410 Al a I Ie O31n I Ie Al a 490 Al a O31n Ar g As n Ser 235 O31u Ly s Ph e Al a As n 315 Ser O31u Al a Al a Pr a 395 O31u O31y O31u Al a O31u 475 Ly s Al a Le u As n I Ie 220 li1e Le u O31u Tr p O31y 300 Al a Le u Al a Le u Ar g 380 ValI Le u O31y O31u Le u 460 ValI Le u Le u Al a Thr 205 Ph e Ser Al a ValI O31y 285 Ar g Ly s Thr vb t As p 365 As n As p Al a vb t Ser 445 li1e Le u Al a As p O31y 1 Tyr O31u O31y Tyr O31y 270 li1e Le u Ser Al a Al a 350 O31u Thr Pr a As n Al a 430 Al a O31y Ly s O31n Al a 510 Thr I Ie Tyr Tyr Thr 255 Le u Ser Le u O31n O31n 335 Al a Al a O31n Tr p Ar g 415 O31n Al a ValI ValI Le u 495 Le u Pr o (3y As p Le u As p (31n As n 525 Page 807 Gene Sequence 24-2-06.txt Leu Leu Lys Leu Ala Val G u 545 I le Thr Al a Asp Leu 625 Al a Ser Leu SIle SIle 705 Thr G u Ser Thr Ser G u 595 Hi s vet Ar g Al a Al a 675 Pr o Pr o Leu Al a Ser 565 Val Ar g Ar g Val Al a 645 Gy Gy Asp Gy Al a 725 Asp 535 G u Val Ar g Pr o G n 615 Val Asp Leu Asp G n 695 Val Leu Ala Ala Arg Ala Val Lys SIle 585 I le Val Pr o Asp Leu 665 I le Leu Al a Phe Gy 555 G u Leu SIle Al a Phe 635 Hi s Pr o Val Asp Ser 715 Leu Lys 540 Ar g Val Al a Al a Ser 620 G n Val G u Thr Met 700 Al a Asp Ala Thr Ile Gy Hi s Gy Asp Lys 605 Al a Thr Val Leu Val 685 Gy l Ie Val G u Lys Al a 590 bet Tyr Pr o Gy Lys 670 Gy Al a Asp Asp Al a G u 575 Phe Gy Al a Al a vet 655 Lys Gy Al a Leu Val 735 <210> 629 <211> 2098 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(2098) <223> FRXA00148 <400> 629 ctttgagggc agcgcgcatg cgcccgatgg ttatttgaac atgacaattg atgccgcggc gacgctggct gacctgctag atgctttggg agcttaaatc atg acg tcg atc cct 115 IVet Thr Ser Ile Pro 1 aat ttt tca gac atc cca ttg act gct gag aca cgt gca tcg gag tca 163 Asn Phe Ser Asp lie Pro Leu Thr Ala G u Thr Arg Ala Ser u Ser 15 cac aac gtt gac gcc ggc aag gtg tgg aac act ccc gaa ggc att gat 211 His Asn Val Asp Ala G y Lys Val Trp Asn Thr Pro G u Gy Ile Asp 30 Page 808 Gene Sequence 24-2-06.txt gtc aag Val Lys gga cat G y Hi s ccg tac Pro Tyr gca ggc Al a G y ctt gct Leu Al a cac cgc Hi s Ar g atg gcc [et Al a 135 gat ggc Asp y 150 gct gtg Al a Val ggt gtg G y Val aaa gaa Lys G u atg cgc Ibet Arg 215 cgt ttt Arg Phe 230 gcg act Al a Thr tac at c Tyr Ile cct cgt Pro Ar g atc gca I e Al a 295 cgc Ar g ccg Pr o cca Pr o ttt Phe gcg Al a ggt Sy 120 ggc G y at t SIle ct g Leu ggt G y ttt Phe 200 at c SIle aac Asn gcc Al a cgt Ar g ct g Leu 280 ttc Phe gat Asp at g et acc Thr caa G n gac Asp gcg Al a ttg Leu at t SIle 170 gag G u gt g Val t cc Ser at t SIle ttg Leu 250 ggt Sy ttc Phe acg Thr t ct Ser tac Ty r 75 gcc Al a aaa Lys tcg Ser at t l Ie t cc Ser 155 ctt Leu cag G n cgc Ar g aac Asn tcg Ser 235 gag G u aaa Lys tt c Phe cag G n ttg Leu 60 acc Thr gcg Al a ggt G y gat Asp gat Asp 140 agc Ser gcg Al a ctt Leu aac Asn at c SIle 220 at t SIle ct g Leu gag G u tgg Tr p gga Gy 300 gct gac cgc Al a Asp Arg 45 cca ggt caa Pro y G n aat cag ccg Asn n Pro gaa t cc aat G u Ser Asn 95 ttg tcg gtt Leu Ser Val 110 aat gag cgc Asn u Arg 125 tcg att ttg Ser Ile Leu gtg tcg gtg Val Ser Val ttc tat atc Phe Tyr Ile 175 gcg ggc acg Al a G y Thr 190 acc tat at t Thr Tyr Ile 205 ttt gag tac Phe G u Tyr tt ggc t at Ser G y Tyr gcc t ac act Al a Tyr Thr 255 gta ggc ctt Val G y Leu 270 ggt att t ct G y Ile Ser 285 gac gag gcg caa gcg gcg G n Ala Ala Asp U U aag Lys tgg Tr p 80 gcg Al a gcg Al a gt g Val gat Asp tcg Ser 160 gt g Val at c SIle t at Tyr acc Thr cac Hi s 240 ct g Leu gac Asp at g bet cca Pr o acg Thr ttt Phe ttc Phe gt c Val at g bet 145 at g Net gcg Al a cag G n ccg Pr o t cc Ser 225 at c SIle gcg Al a gt g Val t ac Tyr AI a ttt Phe at t SIle t at Tyr gat Asp ggc G y 130 cgt Ar g acc Thr gct Al a aat Asn ccg Pr o 210 ttg Leu cag G n gat Asp gat Asp acc Thr 290 cgc Ar g cag G n agg Ar g 100 gcg Al a gt g Val ct g Leu aat Asn gaa G u 180 at c SIle ccg Pr o at g bet gcg Al a at t SIle 260 ttc Phe at g bet ggg a y t ac Tyr aac Asn acc Thr ggt G y ttt Phe ggc G y 165 caa G n ttg Leu tcg Ser cca Pr o gga G y 245 gaa G u gcg Al a gag G u 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 aag ctg cgt gcg Lys Leu Arg Ala cga ctg ctg tgg agc gag ttg gtg gca Arg Leu Leu Trp Ser G u Leu Val Ala 305 Page 809 Gene Sequence 24-2-06.txt aaa Lys 310 cag G n gcc Al a tcg Ser ttc Phe tct Ser 390 gag G u gag G u cct Pr o gat Asp gaa G u 470 gca Al a gcg Al a gag G u gt c Val ttg Leu 550 ggc G y gat Asp t ct Ser acc Thr cac Hi s 360 gct Al a acg Thr ttg Leu gag G u ct g Leu 440 ggc Gy gag G u cag G n gt c Val aaa Lys 520 gct Al a gtt Val t ac Tyr aaa aac gcc aag tcc Lys Asn Ala Lys Ser 315 tgg tcg ttg acc gcg Trp Ser Leu Thr Al a 330 att gag gcg atg gct I e G u Ala Vet Al a 350 aat gca ctt gat gag Asn Al a Leu Asp G u 365 atc gcc cga aac acc lie Ala Arg Asn Thr 380 cgt cca gtt gat cca Arg Pro Val Asp Pro 395 aat gag ctg gct aac Asn G u Leu Ala Asn 410 gcc ggc gga atg gcg Al a G y G y et Al a 430 att gag gaa tca gcg Ile G u u Ser Al a 445 cag gcg ctg atc ggc G n Al a Leu Ile G y 460 att gaa gtc ctc aag lie G u Val Leu Lys 475 gct aaa ctc gcg caa Ala Lys Leu Ala G n 490 gct gcg ctg gat gcg Ala Ala Leu Asp Ala 510 cca ggg gat ttg gat Pro G y Asp Leu Asp 525 cgc gca aaa gct acc Arg Ala Lys Ala Thr 540 ttt ggc cgc cac gaa Phe G y Arg His G u 555 gat gag gtt gga aag Asp G u Val y Lys 570 cag tcg G n Ser 320 cag gat Sn Asp 335 gca acc Al a Thr gcg ttg Al a Leu cag ctg G n Leu tgg gcg Trp Al a 400 cgc gcg Arg Ala 415 cag gcc G n Al a gca cgc Al a Ar g gt g aat Val Asn gtt gac Val Asp 480 ctg aaa Leu Lys 495 ttg aca Leu Thr cag aac Sn Asn att gga I le y gca gaa Al a G u 560 gaa ggc Gu Gy 575 ctg cgc acg cac tcg Leu Arg Thr His Ser 325 gtg tac aac aac gt c Val Tyr Asn Asn Val 340 cag ggc cac acc cag Sn G y His Thr G n 355 gcg ctg ccc acc gat Ala Leu Pro Thr Asp 370 ttg ctg cag cag gaa Leu Leu G n G n G u 385 ggc tcc tat tac gtg G y Ser Tyr Tyr Val 405 cgc aag cac atc gat Arg Lys His lie Asp 420 acc gcg cag gga att Thr Ala n G y Ile 435 acc cag gct cgc att Thr n Ala Arg lie 450 cgc tac gtg gcg gaa Arg Tyr Val Ala G u 465 aac acc aag gtt cgc Asn Thr Lys Val Arg 485 gca gag cgc aac gat Ala G u Arg Asn Asp 500 gct gct gcc cgc aac Ala Ala Ala Arg Asn 515 ctg ctc aaa ctt gcc Leu Leu Lys Leu Ala 530 gag atc tcc gat gct G u Ile Ser Asp Ala 545 atc agg acg ctg tct lie Arg Thr Leu Ser 565 aca gtg agc aac gtc Thr Val Ser Asn Val 580 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 Page 810 g aa O31u cgc Ar g gga O31y g at As p 630 gt g ValI c ac Hi s gcg A a cgt Ar g 600 aag Ly s gga O31y gcc A a ac c Thr gcc A a tt t Ph e gt c ValI ct g Le u gt t ValI 650 ct g Le u gct A a gcc A a t ct Ser 620 c aa O31n gt g ValI ene Sequence 24- 2- 06. txt gac gcc t tt gag gct gag gaa Asp A a Phe G3 u A a G3 u G3 u 590 595 aag at g ggc cag gat gga cat Lys 'b t (31y O31n Asp O31y Hi s 605 610 gcc t at gct gac ct g ggc at g A a Tyr A a Asp Leu 03 y l'b t 625 act cca gcc gaa gct gcc cgc Thr Pr o Ala O31u Ala Ala Ar g 640 gt g ggt at g t ct t cg ct g gca Val O31y l'b t Ser Ser Leu Ala 655 ggc O31y g ac As p g ac As p gcc A a gca A a 660 cgc Ar g cgt Ar g gt g ValI gcc A a 645 ggc (31y 1891 1 939 1 987 2035 2083 2098 <210O> 630 <21 1> 666 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 630 l'bt Thr Ser I Ie Pro As n Ar g Pr o O31u Pr o Thr Ph e Ph e ValI vb t 145 vb t A a Ser O31y O31n vb t Ar g Ar g Le u 115 As p O31n vb t (31u Ser As p A a O31y Tyr As n Thr O31y Ph e O31y 1 65 O31n Hi s ValI O31y Pr o 70 A a Le u Hi s vb t As p 150 A a O31y Ph e As n Ly s Hi s Tyr O31y A a Ar g A a 135 O31y ValI ValI As p As p ValI ValI Thr Ser O31y 1 05 Tyr ValI As p Pr a Pr a 1 85 lIe 10 A a Ph e As p vb t Thr 90 O31n As p A a Le u I Ie 1 70 Pr a O31y Thr Ser Tyr 75 A a Ly s Ser I Ie Ser 1 55 Le u Le u Ly s O31n Le u Thr A a O31y As p As p 140 Ser A a Thr ValI A a Pr a As n O31u Le u As n 125 Ser ValI Ph e A a Tr p As p O31y O31n Ser Ser 110 O31u I Ie Ser Tyr O31u As n Ar g O31n Pr a As n ValI Ar g Le u ValI I Ie 1 Thr Thr As p Ly s Tr p A a A a ValI As p Se r 1 ValI 03 u 03 n L eu A a O31y Th r I Ie 1 Page 811 O31n As n Asp 1 95 I I e Leu Lys 03 u ene Sequence 24- 2- 06. t xt Phe l'bt Val Arg Asn Thr Tyr lie Tyr 200 205 Pr o Ser 225 li1e A a ValI Tyr Ser 305 Le u ValI O31n A a Le u 385 O31y Ar g Thr Thr Ar g 465 As n A a A a Le u (31u 545 Ly s Ly s O31u O31y Ly s 275 Ph e Le u Thr As n Hi s 355 Pr o O31n Tyr Hi s O31n 435 A a ValI Ly s Ar g A a 515 Ly s Ser Ser Pr o O31y 245 (31u A a (31u A a Ser 325 ValI O31n As p O31u ValI 405 As p I Ie I Ie O31u Ar g 485 As p As n A a A a vb t Ar g 230 A a Tyr Pr a I Ie Ly s 310 O31n A a Ser Ph e Ser 390 O31u O31u Pr a As p O31u 470 A a A a O31u ValI Le u 550 Ar g 215 Ph e Thr I Ie Ar g A a 295 Ph e Thr Ar g Le u Ser 375 O31y Tr p ValI Ly s Ser 455 As p O31u O31u Hi s As p 535 (31u I Ie As n A a Ar g Le u 280 Ly s As p Ser Thr Hi s 360 A a Thr Le u O31u Le u 440 O31y O31u O31n ValI Ly s 520 A a ValI I Ie Ser As p A a 265 Ser Le u Pr a O31y A a 345 Thr Ar g ValI Thr O31u 425 Ar g Ar g O31u Le u Ly s 505 O31u A a ValI Ser I Ie Le u 250 O31y Ph e Ar g Ly s Tr p 330 li1e As n I Ie Ar g As n 410 A a I Ie O31n I Ie A a 490 A a Pr a Ar g As n Ser 235 O31u Ly s Ph e A a As n 315 Ser O31u A a A a Pr a 395 O31u O31y O31u A a O31u 475 Ly s A a O31y A a I Ie 220 li1e Le u O31u Tr p O31y 300 A a Le u A a Le u Ar g 380 ValI Le u O31y O31u Le u 460 ValI Le u Le u As p Ly s 540 Ph e Ser A a ValI O31y 285 Ar g Ly s Thr vb t As p 365 As n As p A a vb t Ser 445 li1e Le u A a As p Le u 525 A a O31u O31y Tyr O31y 270 li1e Le u Ser A a A a 350 O31u Thr Pr a As n A a 430 A a O31y Ly s O31n A a 510 As p Thr Tyr Tyr Thr 255 Le u Ser Le u O31n O31n 335 A a A a O31n Tr p Ar g 415 O31n A a ValI ValI Le u 495 Le u O31n I Ie Thr Hi s 240 Le u As p vb t Tr p Ser 320 As p Thr Le u Le u A a 400 A a A a Ar g As n As p 480 Ly s Thr As n O31y (31u 560 Phe 03 y 555 Arg His Ou Ala Page 812 I Ie Thr Al a As p Le u 625 Al a Ser Thr Ser O31u 595 Hi s vb t Ar g Al a Ser 565 ValI Ar g Ar g ValI Al a 645 G31y ValI Ar g Pr o O31n 615 ValI As p Le u ene Sequence 24- 2- 06. txt Tyr Lys Asp O31u Val G31y Lys 570 Al a Il e Al a Leu Al a Asp Al a 585 590 Ar g Ilie Phe I Ie Al a Lys I'b t 600 605 Lys Val Val Al a Ser Al a Tyr 620 G31y Pr o Leu Phe O31n Thr Pr o 635 Al a Asp Val Hi s Val Val G31y 650 Thr Leu Leu 665 O31u 575 Ph e G31y Al a Al a vb t 655 <210O> 631 <21 1> 1971 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 ODS <222> (1948) <223> RXA00149 <400> 631 tt t tcgt agg t aaacacagg t gaaggct tt acaagct tgt at ccaat agc t at ccat aag caagagaaag t aagt ct acg gaactcccta cacaaaagca act gat ct c Thr Asp Leu aag Ly s aag Ly s g ac As p ggt G31y aaa Ly s gt t ValI g ac As p gcg Al a gt g ValI gcc Al a g at As p act Thr ggt G31y ccg Pr o ccc Pr o ggt G31y g at As p aat As n gt t ValI cgc Ar g aat As n g aa O31u tt c Ph e t gg Tr p ct g Le u ggt G31y ggt G31y aac As n ct t Le u gcg Al a aag Ly s 45 t ac Tyr gag O31u t gg Tr p gct Al a t ca Ser cgc Ar g aaa Ly s ac c Thr tt t Ph e ggt G31y gcg Al a 110 gag O31u 15 ac a Thr ct c Le u ag a Ar g ccc Pr a gt t ValI 95 gt g ValI aac As n c ag O31n at c I Ie gca Al a tt c Ph e 80 act Thr ct g Le u g aa (31u aaa Ly s act Thr gag (31u ag g Ar g act Thr gct Al a t gg Tr p at c I Ie ccg Pr a c ag O31n ac c Thr gga O31y 1 00 aat As n ac a Thr t ac Tyr O31y g at As p ag g Ar g act Th r cat Hi s t ct Se r Page 813 Gene Sequence 24-2-06.txt ggc acc acc aca ttg ggt ttt Sy Thr Thr Thr Leu y Phe gag G u ttc tct gag gaa Phe Ser G u G u ttc acg gca gct Phe Thr Al a Al a gat Asp ct g Leu 150 acg Thr tct Ser gaa G u cgc Ar g gat Asp 230 cgt Ar g gt g Val aag Lys ggt Gy gcg Al a 310 gtt Val cgt Ar g aat Asn gag G u 120 aaa Lys cat Hi s gcg Al a cct Pr o gca Al a 200 at c SIle caa G n ttg Leu ttc Phe cgt Ar g 280 cca Pr o ttt Phe gct Al a ttt Phe gct Al a 360 cat Hi s gct Al a ggt Gy gaa G u 170 acg Thr cag G n gt g Val at t SIle gat Asp 250 ttt Phe gct Al a ct g Leu cag G n gct Al a 330 gat Asp cgc Ar g ggt Gy ct c Leu gga Gy 155 gcc Al a gcg Al a tt g Leu gat Asp ggt Gy 235 gca Al a gcg Al a cga Ar g gcc Al a cgt Ar g 315 gca Al a gcg Al a at c SIle cac Hi s gaa G u 140 t cc Ser gga Gy cag G n gca Al a ggt Gy 220 ct a Leu ggc Sy gt c Val cgt Ar g gt a Val 300 gat Asp ggc G y at c l Ie gcg Al a gt g Val 380 gt g Val t ca Ser ttt Phe gat Asp 190 aat Asn agt Ser at c SIle t cc Ser gat Asp 270 tgg Tr p cca Pr o tgg Tr p ggt G y gat Asp 350 aat Asn gat Asp t at Tyr gag G u ttt Phe 175 ggt G y gct Al a ttt Phe gcc Al a acg Thr 255 gag G u gcc Al a cag G n gt g Val gga G y 335 gga G y act Thr cct Pr o ct c Leu gtt Val 160 gct Al a tcg Ser t cc Ser t cc Ser gcc Al a 240 gaa G u cag G n agg Ar g cat Hi s aat Asn 320 gca Al a gtt Val aat Asn gct Al a aac Asn 145 gca Al a gcg Al a cac Hi s aag Lys aac Asn 225 ggt Sy gct Al a ttc Phe gt g Val gcg Al a 305 at g Met acc Thr cct Pr o ttg Leu ggt 35y 385 130 at g Met gcg Al a ttg Leu agt Ser cgt Ar g 210 cag G n gt g Val gca Al a gcg Al a tgt Cys 290 gt g Val ct g Leu gat Asp gga G y ttg Leu 370 gct Al a gcg Al a acc Thr gac Asp 195 gcg Al a ggc Gy gat Asp ctt Leu cag G n 275 gag G u act Thr cgc Ar g gt g Val gt g Val 355 ttg Leu ccg Pr o ttg Leu ttg Leu 180 acc Thr aat Asn gcg Al a t at Tyr aag Lys 260 at t SIe gt g Val gca Al a agt Ser gag G u 340 tcg Ser ct a Leu 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 gga tca tat ttc G y Ser Tyr Phe Page 814 Gene Sequence 24-2-06.txt gtg gag agc ttc acc Val G u Ser Phe Thr 390 agt Ser acg Thr gt g Val ttg Leu gt g Val 470 gct Al a ct g Leu ct g Leu ccc Pr o gt g Val 550 gt c Val gcg Al a aac Asn ttg Leu at c SIle act Thr tcg Ser 440 gag G u cga Ar g ttg Leu ccg Pr o gct Al a 520 act Thr tgt Cys aag Lys aag Lys aca Thr 600 gct Al a gct Al a 410 at g Net aag Lys ccg Pr o gca Al a aag Lys 490 t cc Ser ggt Gy gct Al a acg Thr cgc Ar g 570 ttt Phe gat Asp gat gat cta gcg gag aag gcg Asp Asp Leu Ala G u Lys Al a 395 400 gag ggc gga tac agt gca gct G u G y G y Tyr Ser Al a Al a 415 ctt gat cag acg tgg gag cag Leu Asp n Thr Trp u n 430 aag aag ctc act gga att aat Lys Lys Leu Thr G y Ile Asn 445 ctg cca gct gat cgt cgg gta Leu Pro Ala Asp Arg Arg Val 460 465 gcg gat ttt gaa gcg ctg cgc Al a Asp Phe G u Ala Leu Arg 475 480 aac ggc gcg agg cca cag atc Asn G y Ala Arg Pro Gn lie 495 aag cac aat att cgc act ggt Lys His Asn lie Arg Thr G y 510 ggc att gaa gca atc aac ccg G y lie G u Al a lle Asn Pro 525 ttt gca gaa gct gca cag gcc Phe Ala G u Ala Ala G n Al a 540 545 gac caa gag tat gcc gaa acg Asp n G u Tyr Al a G u Thr 555 560 gaa gcg ggc gtt gag cgc atc Su Ala y Val u Arg Ile 575 gag ggc agc gcg cat gcg ccc G u G y Ser Ala His Ala Pro 590 gcc gcg gcg acg ctg gct gac Al a Ala Ala Thr Leu Ala Asp 605 tgg gct gtg ttc Trp Al a Val Phe tgt Cys act Thr gag G u 450 gaa G u aat Asn acg Thr ttt Phe ggt Gy 530 gca Al a ggg Gy ct g Leu gat Asp gca Al a cgc Ar g 435 ttc Phe cct Pr o cgt Ar g at g bet act Thr 515 caa G n ggc Gy gag G u ctt Leu ggt G5y 595 t cc Ser 420 gct Al a ccg Pr o gca Al a tcg Ser at t SIle 500 t cc Ser ctt Leu at t SIle gga Gy gct Al a 580 t at Tyr ggc Gy gat Asp aac Asn ggt Gy gat Asp 485 cct Pr o aac Asn gtt Val gt a Val gcc Al a 565 ggc Gy ttg Leu 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1971 ctg cta gat gct Leu Leu Asp Ala 610 taaatcatga cgtcgatccc taa <210> 632 <211> 616 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 632 Leu Thr Asp Leu Thr Lys Thr Ala Val Pro G u G u Leu Ser G u Asn Page 815 Gene Sequence 24-2-06.txt Leu Lys Val Asp Thr G u Asn G u Asn 145 Al a Al a Hi s Lys Asn 225 Gy Al a Phe Val Al a 305 vet Thr Pr o Thr Asp Thr Ser Gy Phe Leu 115 Thr Al a Al a Thr Asp 195 Al a Gy Asp Leu G n 275 G u Thr Ar g Val Val 355 Tyr Gy Asp Ar g Thr Hi s Ser Al a Leu Tyr 165 Gy I e Val Ser Val 245 G n Ser Leu Ar g Thr 325 Val Ar g Al a SIle Val Phe Asp Ser Thr Leu 135 I le Leu Ar g G u Al a 215 Al a Ar g Al a Leu Phe 295 bet Al a Thr Phe Val Al a 40 Asp Thr Gy Pr o Thr 120 Lys Hi s Al a Pr o Al a 200 l le G n Leu Phe Ar g 280 Pr o Phe Al a Phe Al a 360 Al a y Val Val Asp Val li e Asn Pr o G u Val Pr o 75 Su Arg Val 90 Lys Asn Il e 105 Thr Leu G y Val Al a Leu Al a G y G y 155 G u G u Al a 170 Leu Thr Al a 185 Val G n Leu Leu Val Asp G u lie y 235 Val Asp Al a 250 Arg Phe Al a 265 Val Al a Ar g G u Leu Al a Ser G n Arg 315 Phe Al a Al a 330 Asp Asp Al a 345 His Arg Ile Phe Al a Ar g Trp Lys Lys Leu Tyr Thr G y G u Phe Sy Trp y Asn Al a Al a 110 Phe G u Phe 125 G u G y Val 140 Ser Thr Ser G y Thr Phe G n Val Asp 190 Al a Val Asn 205 G y Ser Ser 220 Leu Ser II e G y Leu Ser Val Thr Asp 270 Arg Leu Trp 285 Val Ala Pro 300 Asp Pro Trp Sy Val y li e Pro Asp 350 Thr Leu Ar g Pr o Val Val Ser Tyr G u Phe 175 Gy Al a Phe Al a Thr 255 G u Al a G n Val Gy 335 Gy G n SIe Al a Phe Thr Leu G u Leu Val 160 Al a Ser Ser Ser Al a 240 G u G n Ar g Hi s Asn 320 Al a Val Al a Ar g 365 Asn Thr Asn Page 816 Leu Leu Leu Leu G u G u Ser 370 375 Gene Sequence 24-2-06.txt His Leu G y His Val Val Asp Pro Ala 380 Gy 385 Al a Al a G n Asn Val 465 Ar g SIle Gy Pr o Al a 545 Thr SIle Pr o Asp Ser Al a Al a Ar g 435 Phe Pr o Ar g bet Thr 515 G n Gy G u Leu Gy 595 Leu Phe Phe 405 Gy Asp Asn Gy Asp 485 Pr o Asn Val Val Al a 565 Gy Leu Val 390 Ser Thr Val Leu Val 470 Al a Leu Leu Pr o Val 550 Val Al a Asn G u Gy Val Al a Al a 455 Ar g Phe Gy Leu Gy 535 Val G u Pr o let Phe G u Al a 425 Ar g Ser Tr p G u Leu 505 Ser Asp Gy Leu Ser 585 I le Thr Al a 410 Met Lys Pr o Al a Lys 490 Ser Gy Al a Thr Ar g 570 Phe Asp Asp 395 G u Leu Lys Leu Al a 475 Asn Lys Gy Phe Asp 555 G u G u Al a Asp Gy Asp Lys Pr o 460 Asp Gy Hi s SIle Al a 540 G n Al a Gy Al a Leu Gy G n Leu 445 Al a Phe Al a Asn G u 525 G u G u Gy Ser Al a 605 Al a Tyr Thr 430 Thr Asp G u Ar g SIle 510 Al a Al a Tyr Val Al a 590 Thr G u Ser 415 Tr p Gy Ar g Al a Pr o 495 Ar g SIe Al a Al a G u 575 Hi s Leu Asp Al a Leu G y Al a 615 <210> 633 <211> 777 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(754) <223> RXN00317 <400> 633 caacggttac atcactcacg cattaactcc cctggttggt accgcatgtt cgtttcccaa gtctagccaa gccttgaaaa attctggcaa ggttaatggt gtg act acg cct tct 115 Val Thr Thr Pro Ser 1 aag aaa act ctg ctc ttt gat ctc gac gga acc ctc gtc gat tct ttc 163 Lys Lys Thr Leu Leu Phe Asp Leu Asp G y Thr Leu Val Asp Ser Phe Page 817 Gene Sequence 24- 2- 06. txt 15 ccc ggt atc cgc act tca ttc ctt cac acc ctg cac gaa aag aac tgg 211 Pro G y lie Arg Thr Ser Phe Leu His Thr Leu His G u Lys Asn Trp 30 gaa atc ccc tct gag gaa cgc atc tcg caa gtt cca gga cct ccc atg 259 Su Ile Pro Ser u u Arg Ile Ser n Val Pro y Pro Pro Met 45 gaa tgg acg ttc cag gat ttg ggc atg act cca gag cag gca caa gac 307 Su Trp Thr Phe n Asp Leu y Met Thr Pro u n Ala n Asp 60 gct ctg cag acc tac ctt gag cat tac ggc cag gtg ggt tgg gat ctt 355 Ala Leu G n Thr Tyr Leu G u His Tyr G y G n Val G y Trp Asp Leu 75 80 tcc gaa gca ttc ccc ggc atg cga gat ttg ctg atc cgc ttg aaa tac 403 Ser G u Ala Phe Pro G y Iet Arg Asp Leu Leu lie Arg Leu Lys Tyr 95 100 gaa ggt ttc cgt ctg tgc acc gcc acc tcc aag ggc gag ttc ttt gcg 451 G u y Phe Arg Leu Cys Thr Ala Thr Ser Lys y G u Phe Phe Ala 105 110 115 gag aag gta ctt cgc aaa ttc gag atg ttc gat ctc ttc gaa ttc atg 499 G u Lys Val Leu Arg Lys Phe G u Iet Phe Asp Leu Phe G u Phe Iet 120 125 130 ggt gcc gcc acc gac agc ggc aac cga cgc agc aaa tct gcc gtg atc 547 G y Ala Ala Thr Asp Ser y Asn Arg Arg Ser Lys Ser Ala Val lie 135 140 145 aaa cat gtc ctc gac agc gtt ggg ttg gac gaa cca aat gat att ttg 595 Lys His Val Leu Asp Ser Val y Leu Asp G u Pro Asn Asp Ile Leu 150 155 160 165 atg att ggt gat cga tca cac gat att gaa ggt tcg agt gaa ttc ggc 643 IVet IIe G y Asp Arg Ser His Asp IIe G u G y Ser Ser G u Phe G y 170 175 180 atc gat tgt gtt gcc gta acc tgg ggc tac ggc agc aaa act gaa tgg 691 Ile Asp Cys Val Ala Val Thr Trp y Tyr y Ser Lys Thr G u Trp 185 190 195 gac gct gcc cgc tac acc gtg agc acc gca gaa gaa tta gaa agg atc 739 Asp Ala Ala Arg Tyr Thr Val Ser Thr Ala G u G u Leu G u Arg lie 200 205 210 atc cat gac tgg gcc taaaacttcg ctacctgtgg aaa 777 lie His Asp Trp Ala 215 <210> 634 <211> 218 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 634 Val Thr Thr Pro Ser Lys Lys Thr Leu Leu Phe Asp Leu Asp y Thr 1 5 10 Leu Val Asp Ser Phe Pro y lie Arg Thr Ser Phe Leu His Thr Leu 25 His u Lys Asn Trp Ou lie Pro Ser u u Arg lie Ser n Val Page 818 Gene Sequence 24-2-06.txt Thr Phe G n Asp Gy lMt Thr Pro Pro G y G u G n Val G y li e Ar g G y G u Leu Phe 130 Lys Ser 145 Pro Asn Ser Ser Ser Lys G u Leu 210 Pro Pro let u Al a Tr p Leu Phe 115 G u Al a Asp G u Thr 195 G u Asp Leu Tyr Al a et l Ie Leu 165 Gy Tr p SIle Al a 70 Ser G u G u Gy Lys 150 bet SIle Asp SIle Tr p Leu G u Gy Lys Al a 135 Hi s SI e Asp Al a Hi s 215 n Al a Phe Val 120 Al a Val Gy Cys Al a 200 Asp Thr Phe Ar g 105 Leu Thr Leu Asp Val 185 Ar g Tr p Ty r Pr o 90 Leu Ar g Asp Asp Ar g 170 Al a Tyr Al a Leu Gy Cys Lys Ser Ser 155 Ser Val Thr Hi s Ar g Al a G u 125 Asn Gy Asp Tr p Ser 205 Gy Leu Ser Phe Ar g Asp G u 175 Tyr Al a <210> 635 <211> 439 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(439) <223> FRXA00317 <400> 635 caacggt t ac atcactcacg cat t acctcc cctggt t ggt gtcaagccaa gccttgaaaa attctggcaa ggttaatggt aag Lys ccc Pr o gaa Gu gaa Gu act Thr at c SIle ccc Pr o acg Thr ct c Leu act Thr gag G u cag G n ttt Phe t ca Ser gaa Gu gat Asp gat Asp ttc Phe cgc Ar g ttg Leu gac Asp cac Hi s tcg Ser at g Net gga acc G y Thr 15 acc ct g Thr Leu caa gtt G n Val act cca Thr Pro Page 819 accgcatgtt cgtttcccaa gtg act acg cct tct Val Thr Thr Pro Ser 1 ctc gtc gat tct ttc Leu Val Asp Ser Phe cac gaa aag aac tgg His G u Lys Asn Trp cca gga cct ccc atg Pro y Pro Pro IVet gag cag gca caa gac Su G n Ala n Asp Gene Sequence 24- 2- 06. txt gct ctg cag acc tac ctt gag cat tac ggc cag gtg ggt tgg gat ctt 355 Ala Leu G n Thr Tyr Leu G u His Tyr G y G n Val G y Trp Asp Leu 75 80 tcc gaa gca ttc ccc ggc atg cga gat ttg ctg atc ccc ttg ata tac 403 Ser G u Ala Phe Pro G y Iet Arg Asp Leu Leu lie Pro Leu Ile Tyr 95 100 gaa ggt ttt cgt ctg tgc acc gcc acc ttt caa ggg 439 Su G y Phe Arg Leu Cys Thr Ala Thr Phe G n y 105 110 <210> 636 <211> 113 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 636 Val Thr Thr Pro Ser Lys Lys Thr Leu Leu Phe Asp Leu Asp Gy Thr 1 5 10 Leu Val Asp Ser Phe Pro Gy lie Arg Thr Ser Phe Leu His Thr Leu 25 His u Lys Asn Trp Ou lie Pro Ser u u Arg lie Ser n Val 40 Pro y Pro Pro Iet G u Trp Thr Phe G n Asp Leu Gy IVet Thr Pro 55 G u G n Ala n Asp Ala Leu G n Thr Tyr Leu G u His Tyr G y G n 70 75 Val G y Trp Asp Leu Ser G u Al a Phe Pro G y Iet Arg Asp Leu Leu 90 lie Pro Leu Ile Tyr G u y Phe Arg Leu Cys Thr Ala Thr Phe G n 100 105 110 Gy <210> 637 <211> 816 <212> DNA <213> Cor ynebact er i um gl ut ai cum <220> <221> CDS <222> (101)..(793) <223> RXA02196 <400> 637 tctaccaccc agaggcaaag tactttaacg tctaacacct ttgagaggga aaactttccc gcacattgca gatcgtgcca ctttaactaa ggttgacggc atg att aag gcg att 115 IVet Ile Lys Ala lle 1 ttc tgg gac atg gac ggc acg atg gtg gac tct gag cca cag tgg ggc 163 Phe Trp Asp Iet Asp G y Thr IVet Val Asp Ser G u Pro G n Trp G y 15 att gct acc tac gag ctc agc gaa gcc atg ggc cgc cgc ctc acc ccg 211 I e Ala Thr Tyr u Leu Ser G u Al a Iet G y Arg Arg Leu Thr Pro Page 820 Gene Sequence 24- 2- 06. txt 30 gag ctc cgg gaa ctc acc gtc ggc tcg agc ctg ccg cgc acc atg cgc 259 G u Leu Arg G u Leu Thr Val y Ser Ser Leu Pro Arg Thr IVet Arg 45 tta tgc gca gag cac gca ggc att aca ttg agc gac gcg gac tac gag 307 Leu Cys Ala G u His Al a G y IIe Thr Leu Ser Asp Ala Asp Tyr G u 60 cgc tac cgg gct ggc atg ttc gcc cgg gtc cat gag ctt ttc gac gaa 355 Arg Tyr Arg Ala Gy Mbt Phe Ala Arg Val His G u Leu Phe Asp G u 75 80 tcc ctc gtc cca aat cca ggc gtc acc gaa ctc ctg aca gag ttg aag 403 Ser Leu Val Pro Asn Pro y Val Thr G u Leu Leu Thr G u Leu Lys 95 100 gcc ctc gag atc ccc atg ttg gtc acc acc aac aca gag cgc gat ctc 451 Ala Leu G u lie Pro IVet Leu Val Thr Thr Asn Thr G u Arg Asp Leu 105 110 115 gcg acc cgt tca gtc gca gcc gtg gga aat gag ttc ttc atc ggt tct 499 Ala Thr Arg Ser Val Al a Ala Val y Asn G u Phe Phe Ile y Ser 120 125 130 atc gct ggt gat gaa gtc cca aca gca aag cca gcc ccc gac atg tac 547 Ile Al a y Asp u Val Pro Thr Ala Lys Pro Ala Pro Asp Iet Tyr 135 140 145 ctc gaa gca gca cga cgt gtg ggc ttt gac cca tca gag tgc ctc gtg 595 Leu G u Ala Ala Arg Arg Val y Phe Asp Pro Ser G u Cys Leu Val 150 155 160 165 ttc gaa gat tcc tac aac ggc atg ctg ggc gct gtt act gca ggt tgc 643 Phe G u Asp Ser Tyr Asn G y IVet Leu G y Al a Val Thr Al a G y Cys 170 175 180 cgc gtc att ggt ctg cac cca gaa gaa gtc caa gcg cca gaa ggt gta 691 Arg Val IIe G y Leu His Pro G u G u Val G n Ala Pro G u G y Val 185 190 195 gtg cct ttg cgt tcc ctc cac ggt aaa aac tct ttc gaa ggt gtc acc 739 Val Pro Leu Arg Ser Leu His G y Lys Asn Ser Phe G u G y Val Thr 200 205 210 gct gag atg gtc act gcc tgg tac cac cag atc gag ccg gca ggt gtc 787 Al a G u et Val Thr Ala Trp Tyr His n I Ie G u Pro Al a y Val 215 220 225 gca aaa taaaaccagg tgggggagtg aaa 816 Ala Lys 230 <210> 638 <211> 231 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 638 Iet lie Lys Ala lie Phe Trp Asp IVet Asp y Thr IVet Val Asp Ser 1 5 10 Su Pro n Trp y Ile Ala Thr Tyr u Leu Ser u Ala et y 25 Arg Arg Leu Thr Pro G u Leu Arg G u Leu Thr Val y Ser Ser Leu Page 821 Gene Sequence 24-2-06.txt Ala G u His Ala Pr o Asp G u Leu Thr Phe Al a 145 Ser Val Al a Phe G u 225 Thr Asp Phe G u Ar g 115 I le Asp Cys Al a G u 195 Gy Al a Ar g G u G u Lys Leu Ser Tyr Val 165 Cys Val Thr Val Leu Ar g 70 Ser Al a Al a SIle Leu 150 Phe Ar g Val Al a Al a 230 Cys Ty r Leu Leu Thr Al a 135 G u G u Val Pr o G u 215 Lys Al a Pr o SIle 105 Ser Asp Al a Ser Gy 185 Ar g Nbet Pr o vet Al a Val Ar g 155 Asn Hi s Leu Gy Phe Gy Leu Al a Pr o 140 Val Gy Pr o Hi s Tr p 220 Al a Val Val Val 125 Thr Gy vet G u Gy 205 Ile Thr Leu Ser Val G u Thr Asn Lys Asp Gy 175 Val Asn [et Val Thr Al a Tyr His Gn Ile <210> 639 <211> 531 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(508) <223> RXN02461 <400> 639 agaaaatctc aaggcaaaaa acaagccacc ccaatctgtg acgactatta tgtcacgaag aaaccaaaga aagggaaata gtt gac tac gct gga gta cta gac gga Val Asp Tyr Ala y Val Leu Asp y tgg cgc aac ctg ctc gcc gca gca aag Trp Arg Asn Leu Leu Ala Ala Ala Lys 30 atc ctc agc aac gat cca ggt ggg ctc lle Leu Ser Asn Asp Pro Gy Gy Leu 45 acc gat Thr Asp 15 aaa aat Lys Asn ggc gca G y Al a Page 822 cgacaatcaa accacagact atg cgc gga cta att IVet Arg Gy Leu Ile 1 gag gac cag cgt cgc G u Asp G n Arg Arg ggc gtc gga acc gtg Sy Val y Thr Val gcg ccg atc cgg gaa Ala Pro lie Arg u ene Sec ct C gaa aca aac ggg gt a gt C gat aag Leu O31u Thr As n G31y ValI Val Asp Lys 60 ggc gt C gaa aag cca gag gaa gca gct G31y Val (31u Lys Pr o 31u (31u Al a Al a 75 at c gac ct g ccc at g cgt gac t gc gt g Ilie Asp Leu Pr o I'b t Ar g Asp Cys Val aac gt g cgc ggc gcc gt C gaa gcc gga Asn Val Arg G31y Al a Val O31u Al a G31y 105 110 caa t tt gac cgt gca gt c gt c gaa at c O31n Phe Asp Ar g Al a Val Val G31u Ilie 120 125 gga gaa t tc t aat ct tgcg cgt ct acat c c( G3 y G3 u Phe 135 <210> 640 <211> 136 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 640 I'bt Arg Gy Leu lieVal Asp Tyr Ala 1 5 O31u Asp O31n Ar g Ar g Tr p Ar g As n Le u G31y Va 1G31y Th r Val I Ie Leu Ser Asn 40 Al a Pro Ilie Ar g O31u Leu O31u Thr Asn 55 Leu Ser G31y O31u Leu G31y Val O31u Lys 70 Al a Al a Al a Asp Al a I Ie Asp Leu Pr o Asp Asp Ser Ilie Leu Asn Val Ar g G31y 100 105 G31y Val Tyr Tyr O31n O31n Phe Asp Ar g 115 120 Leu Phe 03 y Leu 03 u 03 y 03 u Phe 130 135 <210> 641 <211> 1662 <212> DNA <213> Cor ynebact er i urn gi ut ani curn <220> <221> OIDS <222> (1639) <223> RXN01744 iuence 24-2- 06. txt gt g ct g ct g t cg gga Val Leu Leu Ser 03 y ttc cag gcc gcc gca Phe 03 n Al a Al a Al a ct t gt c gac gac t cg Leu Val Asp Asp Ser 95 ct c gt a ggc gt c t ac Leu Val 03 y Val Tyr 115 gt c gga ct g t tc ggg Val G31y Leu Phe G31y 130 g aa G31u g ac As p at c I Ie 1 00 t ac Tyr ct a Le u G31y 10 Le u As p G31y Pr o vb t 90 Al a Al a ValI Al a Pr o ValI G31u Ar g ValI ValI Le u Al a G31y ValI G31u As p G31u ValI As p Al a G31y As p Al a Cys Al a G31u 125 Thr Ly s G31y ValI Ph e Le u Le u ValI Page 823 Gene Sequence 24-2-06.txt <400> 641 tcttattggt tcttcgtttt gtatcgataa atacaatcgg tttcctggct taataaggct gt t cctgtca acctgcaatg gaagaggaag tgtacctagc gtg gat gtc gtc Val Asp Val Val at c SIle gt c Val ttt Phe ttt Phe at t I e ttc Phe gcg Al a ggg Gy at t SIle at g et 150 gag G u gcg Al a gtt Val aag Lys gcg Al a 230 cgg Ar g ct g Leu caa G n act Thr cag G n ggt Gy ttc Phe at t SIle 120 acg Thr cat Hi s act Thr ccg Pr o gga Gy 200 gct Al a tgg Tr p caa G n at t SIle acc Thr ct g Leu ttc Phe gtt Val gag G u ggt Gy at t SIle gt g Val ttt Phe 170 gct Al a tcg Ser tcg Ser ggt Gy tt c Phe ggc Gy ggc Gy ct c Leu cag G n 75 tt c Phe tct Ser tgg Tr p tct Ser ggt Sy 155 tgg Tr p gtt Val ggt Gy gaa G u tgg Tr p 235 gga Gy tta Leu aaa Lys at c SIle ttc Phe ggc Gy gt a Val ttg Leu gcc Al a 140 gct Al a gct Al a gcc Al a tgg Tr p at c SIle 220 tgg Tr p at t SIle gcg Al a gag G u 45 aac Asn ggt Gy gga Gy ttc Phe cac Hi s 125 t at Tyr gag G u ctt Leu ggt Gy tgg Tr p 205 gag G u acc Thr acc Thr ccg Pr o cac Hi s ttc Phe at g Met ccg Pr o ct g Leu 110 act Thr ttc Phe t at Tyr ct c Leu ggt Gy 190 at t SIle tcg Ser aca Thr acc Thr 15 ct g Leu tgg Tr p gcg Al a aac Asn ct g Leu 95 gga Gy gca Al a at c SIle aac Asn aca Thr 175 ttt Phe at t SIle aag Lys gtt Val gt c Val gt c Val t at Tyr gtt Val tgg Tr p 80 gct Al a ct g Leu t cc Ser at c SIle cct Pr o 160 aac Asn tta Leu cgt Ar g cat Hi s gt c Val 240 1 t at Tyr gca Al a cgg Ar g ggt Gy tcg Ser ttg Leu tgg Tr p at t SIle gt g Val 145 gag G u t cc Ser aca Thr gcg Al a tcg Ser 225 tct Ser cac Hi s at c l Ie gcc Al a gt a Val gaa Gu gag G u at t SIle tgg Tr p 130 gcc Al a act Thr acc Thr gct Al a cac Hi s 210 at g Net t cc Ser ttc Phe at g bet aca Thr gca Al a t at Tyr ggt Gy ttc Phe 115 at c SIle aac Asn ggt Sy gcg Al a gga Gy 195 cgt Ar g cac Hi s gt g Val at t I e caa G n aga Ar g acg Thr tcg Ser ctt Leu 100 gga Gy gtt Val tcg Ser cgt Ar g ct g Leu 180 act Thr cag G n agg Ar g gcg Al a gac Asp ttt Phe acg Thr ttt Phe ggc Gy cgt Ar g at c SIe tgg Tr p gct Al a ttt Phe gcg Al a 165 gct Al a ttc Phe gcc Al a ccc Pr o ctg Leu 245 Page 824 ttc atc act ggc Phe lie Thr G y at g Met aac Asn acc Thr aaa Lys 310 gcg Al a acc Thr gct Al a act Thr ccg Pr o 390 ggc Gy gcc Al a cat Hi s t at Tyr ct g Leu 470 gga Gy at g NMbt t cc Ser 280 ct g Leu acc Thr caa G n tgg Tr p gct Al a 360 aag Lys cca Pr o cag G n aca Thr cca Pr o 440 at t SIle gga Gy gca Al a gat Asp 250 tcg Ser ct g Leu gat Asp gt g Val t ac Tyr 330 ttc Phe at t l Ie gct Al a ttg Leu tgg Tr p 410 at g Met caa G n ttc Phe cca Pr o ccg Pr o 490 gcg Al a aca Thr gtt Val act Thr 315 ggt Gy cgc Ar g gcg Al a cgt Ar g gcc Al a 395 gt g Val at c SIle gt c Val gt g Val gag G u 475 at t SIle Gene Sequence 24-2-06.txt atc cag gcg aag ctc atg ttc gtg caa cag cca Ie G n Ala Lys Leu IVet Phe Val n n Pro gaa G u at t SIle ccg Pr o 300 ttg Leu cct Pr o gca Al a tgg Tr p ct g Leu 380 aac Asn gt g Val cgg Ar g tgg Tr p gt g Val 460 gaa gtu ggt Gy t cc Ser ggt Gy 285 ttt Phe cag G n ggc G y at g Met ct g Leu 365 ttc Phe tct Ser cac Hi s at g et ct g Leu 445 tgg Tr p ggc G y t ca Ser ttg Leu 270 acg Thr gt g Val ggt G y aac Asn at c SIle 350 ttg Leu cag G n gct Al a ccg Pr o act Thr 430 act Thr gt g Val gct Al a gat Asp ggt 10y 510 255 tgt Cys cac Hi s ctt Leu gt a Val t ac Tyr 335 ggc Gy ct g Leu at c SIle ggt Sy aac Asn 415 gtt Val ct c Leu tgg Tr p cca Pr o at g Net 495 gaa G u gaa acc G u Thr aac aac Asn Asn cca ttc Pro Phe 305 aac cag Asn n 320 t cc cct Ser Pro ctg at g Leu IVet cgt aag Arg Lys ggc agc G y Ser 385 tgg atc Trp Ile 400 cct gaa Pro G u gat at g Asp IVet att ggc I e G y ctg att Leu II e 465 tcc gtg Ser Val 480 ccc at g Pro IVet aag gaa Lys G u gcc Al a tgc Cys 290 ttg Leu ct c Leu aac Asn ctt Leu aag Lys 370 ct c Leu ttc Phe tct Ser ggt Sy ttc Phe 450 cgc Ar g gag G u aca Thr aca Thr 275 gat Asp gct Al a caa G n ttg Leu ggt Gy 355 cgc Ar g at t SIle acc Thr gcc Al a gt a Val 435 acg Thr cgc Ar g gca Al a ccg Pr o 260 gat cca Asp Pro acg gta Thr Val gaa gga G u G y gct gca Al a Al a 325 ttt gtc Phe Val 340 tct ttg Ser Leu aca cca Thr Pro gct at c Ala I e gag at g G u let 405 ggc gat Sy Asp 420 tct gat Ser Asp att ctc I e Leu gca gtt Al a Val aag act Lys Thr 485 ctg caa Leu G n 500 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1649 ttt act gcc Phe Thr Al a gcc cca acc aca Ala Pro Thr Thr t aaccat gga Page 825 Gene Sequence 24- 2- 06. txt tctcaatacc ttt <210> 642 <211> 513 <212> PRT <213> Cor ynebact eri um gl ut am cum 1662 <400> 642 Val Asp Val 1 Tyr Hi s Phe Al a ll e et Ar g Al a Thr Sy Val Al a Ser G u Tyr Leu G u G y Trp Ile Phe 115 lie Trp Ile 130 Val Al a Asn 145 G u Thr G y Ser Thr Al a Thr Al a G y 195 Ala His Ar g 210 Ser IVet Hi s 225 Ser Ser Val Phe Val G n Thr Al a Thr 275 Asn Cys Asp 290 Phe Leu Al a 305 Val Asp 5 Ile Phe G n Thr Arg Phe Thr G y Ser Arg Leu II e 100 Sy Trp Val Ala Ser Phe Arg Ala 165 Leu Al a 180 Thr Phe G n Al a Arg Pro Al a Leu 245 G n Pro 260 Asp Pro Thr Val G u G y II e Al a Ar g Val Pro Leu Phe Trp G n 40 Phe G y Thr 55 Ile Val n 70 Phe Val G y Al a Phe Phe G y Lys I I e 120 lI e Al a Thr 135 vet n His 150 G u Leu Thr Ala Phe Pro Val Leu G y 200 Lys Lys Al a 215 Ala Leu Trp 230 Phe Ile Thr et Lys et Asn Phe Ser 280 Thr Hi s Leu 295 Lys Phe Thr 310 Tr p Thr Val Val G u Asp Leu 105 Pr o Asn Pr o Asp Hi s 185 I le G u Val Sy Al a 265 I e I e Gy Phe Sy Sy Leu G n 75 Phe Ser Tr p Ser Sy 155 Tr p Val Sy G u Tr p 235 I le Al a Thr Val Sy Leu Lys SIle Phe Sy Val Leu Al a 140 Al a Al a Al a Tr p SIle 220 Tr p G n Su I e Pr o 300 I le Al a G u Asn Sy Sy Phe Hi s 125 Tyr G u Leu Sy Tr p 205 G u Thr Al a Ser Sy 285 Phe Thr Leu Tr p Al a Asn Leu Sy Al a SIle Asn Thr 175 Phe SIle Lys Val Leu 255 Cys Hi s Leu Val Val Tyr Val Tr p Al a Leu Ser SIe Pr o 160 Asn Leu Ar g Hi s Val 240 et G u Asn Pr o Val Thr Leu G n y Val Asn 315 320 Page 826 Gene Sequence 24-2-06.txt Sn Leu n Ala Ala Al a u Gn Ala Sy Pro y Asn Pr o et Lys Ser 385 I le G u et Gy SIle 465 Val Leu Sy 355 Ar g SIle Thr Al a Val 435 Thr Ar g Al a 325 Val Leu Pr o SIle bet 405 Asp Asp Leu Val Thr 485 Thr Al a Thr Pr o 390 Sy Al a Hi s Ty r Leu 470 Sy Tyr l Ie Sy 375 Phe Ar g Ar g Al a Leu 455 I le Pr o Tr p Al a 360 Lys Pr o G n Thr Pr o 440 I le Sy Al a Ser 345 Al a SIle Phe Pr o G u 425 Tr p Leu Pr o Thr Ar g Al a Ar g Al a 395 Val SIle Val Val G u 475 I le Al a Tr p Leu 380 Asn Val Ar g Tr p Val 460 G u Sy bet Leu 365 Phe Ser Hi s bet Leu 445 Tr p Sy Ser SIle 350 Leu G n Al a Pr o Thr 430 Thr Val Al a Asp Tyr Ser 335 G y Leu Leu Arg I e y Sy Trp 400 Asn Pro 415 Val Asp Leu II e Trp Leu Pro Ser 480 IVet Pro 495 IVet Thr Pro Leu n Phe Thr Ala Ala Ala Pro Thr Thr G y G u Lys 510 G u 505 <210> 643 <211> 238 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(238) <223> FRXA00055 <400> 643 tcttattggt tcttcgtttt gtatcgataa atacaatcgg gt t cctgtca acctgcaatg gaagaggaag tgtacctagc atc gca cgg tgg caa ttc gga att acc acc gtc lie Ala Arg Trp n Phe y I e Thr Thr Val 15 gtc cca ctg acc att ggc tta gca ccg ctg gtc Val Pro Leu Thr Ile y Leu Ala Pro Leu Val 30 ttt tgg caa gtt acc ggc aaa gag cac Phe Trp G n Val Thr y Lys u His tttcctggct taataaggct gtg gat gtc gtc gac Val Asp Val Val Asp 1 tat cac ttc att ttt Tyr His Phe lie Phe gcg atc atg caa acg Ala lle et n Thr Page 827 Gene Sequence 24-2-06. txt <210> 644 <211> 46 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 644 Val Asp Val Val Asp I e Ala Arg Trp On Phe Gy I e Thr Thr Val 1 5 10 Tyr His Phe Ile Phe Val Pro Leu Thr Ile y Leu Ala Pro Leu Val 25 Ala IIe Net n Thr Phe Trp n Val Thr Gy Lys u His 40 <210> <211> <212> <213> <220> <221> <222> <223> 645 1325 DNA Cor ynebact eri um gl ut ai cum CDS (1)..(1302) FRXA01744 <400> 645 tgg Tr p 1 gct Al a ct g Leu t cc Ser at c I e cct Pr o aac Asn tta Leu cgt Ar g cat Hi s gaa G u gag G u at t I e tgg Tr p gcc Al a act Thr acc Thr gct Al a 115 cac Hi s at g bet tcg Ser 5 ctt Leu gga Gy gtt Val tcg Ser cgt Ar g ct g Leu act Thr cag G n agg Ar g cgt ttc gtc ggt Arg Phe Val y gtt ttc ggc gga Val Phe Gy y ccg ctg Pro Leu at c SIle tgg Tr p gct Al a ttt Phe 70 gcg Al a gct Al a tt c Phe gcc Al a ccc Pr o gcg Al a ggg Gy at t lle at g bet gag G u gcg Al a gtt Val aag Lys 135 gcg Al a ttc Phe at t SIle acg Thr cat Hi s act Thr ccg Pr o 105 gga Gy gct Al a tgg Tr p gag G u ggt Gy at t SIle gt g Val 75 ttt Phe gct Al a tcg Ser tcg Ser tct Ser tgg Tr p tct Ser ggt Gy tgg Tr p gtt Val ggt Gy gaa G u 140 gt a Val ttg Leu gcc Al a gct Al a gct Al a gcc Al a tgg Tr p 125 at c SIle ct g Leu act Thr ttc Phe t at Tyr ct c Leu ggt Gy at t SIle tcg Ser gga Gy gca Al a at c SIe aac Asn aca Thr ttt Phe att SIe aag Lys gtt ggt tgg tgg acc aca gtt Val Gy Trp Trp Thr Thr Val Page 828 Gene Sequence 24-2-06.txt gt c Val at g Met gaa G u aac Asn cca Pr o 225 aac Asn t cc Ser ct g Leu cgt Ar g ggc Gy 305 tgg Tr p cct Pr o gat Asp at t SIle ct g Leu 385 t cc Ser t cc Ser gt g Val gcc Al a 195 tgc Cys ttg Leu ct c Leu aac Asn ctt Leu 275 aag Lys ct c Leu ttc Phe tct Ser ggt Gy 355 ttc Phe cgc Ar g gag G u gcg Al a 165 cag G n gat Asp acg Thr gaa G u gct Al a 245 ttt Phe tct Ser aca Thr gct Al a gag G u 325 ggc Gy tct Ser at t SIle gca Al a aag Lys 405 ct g Leu cca Pr o cca Pr o gt a Val gga Gy 230 gca Al a gt c Val tt g Leu cca Pr o at c l Ie 310 at g bet gat Asp gat Asp ct c Leu gtt Val 390 act Thr ttc Phe at g let aac Asn acc Thr 215 aaa Lys gcg Al a acc Thr gct Al a act Thr 295 ccg Pr o ggc Gy gcc Al a cat Hi s t at Tyr 375 ct g Leu gga Gy at c SIle aag Lys ttc Phe 200 cac Hi s ttc Phe gag G u t ac Tyr at c l Ie 280 gga Gy ttc Phe cgc Ar g cga Ar g gcg Al a 360 ct c Leu at c SIle ccg Pr o act Thr at g bet 185 t cc Ser ct g Leu acc Thr caa G n tgg Tr p 265 gct Al a aag Lys cca Pr o cag G n aca Thr 345 cca Pr o at t SIle gga Gy gca Al a ggc Gy 170 gcg Al a at t SIle at c SIle ggt Gy gca Al a 250 t ca Ser gcg Al a at t SIle ttc Phe cct Pr o 330 gag G u tgg Tr p ttg Leu cca Pr o acc Thr 410 155 gat Asp tcg Ser ct g Leu gat Asp gt g Val 235 t ac Tyr ttc Phe at t l Ie gct Al a ttg Leu 315 tgg Tr p at g Net caa G n ttc Phe cca Pr o 395 ccg Pr o at c SIle gcg Al a aca Thr gtt Val 220 act Thr ggt Sy cgc Ar g gcg Al a cgt Ar g 300 gcc Al a gt g Val at c SIle gt c Val gt g Val 380 gag G u at t SIle cag G n gaa G u at t SIle 205 ccg Pr o ttg Leu cct Pr o gca Al a tgg Tr p 285 ct g Leu aac Asn gt g Val cgg Ar g tgg Tr p 365 gt g Val gaa G u ggt Gy gcg Al a t cc Ser 190 ggt Gy ttt Phe cag G n ggc Sy at g let 270 ct g Leu ttc Phe tct Ser cac Hi s at g Net 350 ct g Leu tgg Tr p ggc Sy t ca Ser aag ct c Lys Leu 175 ttg tgt Leu Cys acg cac Thr His gtg ctt Val Leu ggt gta G y Val 240 aac t ac Asn Tyr 255 atc ggc I e y ttg ctg Leu Leu cag at c n Ilie gct ggt Al a y 320 ccg aac Pro Asn 335 act gt t Thr Val act ct c Thr Leu gtg tgg Val Trp gct cca Ala Pro 400 gat at g Asp IVet 415 ggt gaa G y G u 528 576 624 672 720 768 816 864 912 960 1008 1056 1104 1152 1200 1248 1296 ccc atg aca ccg ctg caa Pro IVet Thr Pro Leu G n ttt act gcc gct gcc Phe Thr Ala Ala Ala cca acc aca Pro Thr Thr Page 829 Gene Sequence 24-2-06.txt 420 425 430 aag gaa taaccatgga tctcaatacc ttt Lys G u <210> 646 <211> 434 <212> PRT <213> Corynebacter i um gl ut am cum 1325 <400> 646 Tr p 1 Al a Leu Ser I e Pr o Asn Leu Ar g Hi s 145 Val vet G u Asn Pr o 225 Asn Ser Leu Ser G u Tyr Leu G u G 2C Trp Ile Phe lie Trp IIE Val Al a Asr G u Thr G) Ser Thr Al Thr Al a G 115 Ala His Ar c 130 Ser Net Hi Ser Ser Val Phe Val G r 18C Thr Al a Thr 195 Asn Cys Asp 210 Phe Leu Al G n Leu G r Pro Asn LeL 26C et Leu G 275 Ser Arg Phe Val y 5 Leu Sy Val Ser Ar g Leu Thr G n Ar g Al a 165 G n Asp Thr G u Al a 245 Phe Ser SIle Tr p Al a Phe 70 Al a Al a Phe Al a Pr o 150 Leu Pr o Pr o Val Sy 230 Al a Val Leu Al a Gy l e 55 bet G u Al a Val Lys 135 Al a Phe Met Asn Thr 215 Lys Al a Thr Al a Phe I le Thr Hi s Thr Pr o 105 Sy Al a Tr p Thr bet 185 Ser Leu Thr G n Tr p 265 Al a Asp 10 Leu Pr o Asn Pr o Asp 90 Hi s SIle G u Val Sy 170 Al a SIle SIle Sy Al a 250 Ser Al a Val G u Sy SIle Val 75 Phe Al a Ser Ser Sy 155 Asp Ser Leu Asp Val 235 Tyr Phe l Ie Phe Ser Tr p Ser Gy Tr p Val Sy G u 140 Tr p SIle Al a Thr Val 220 Thr Sy Ar g Al a Sy Val Leu Al a Al a Al a Al a Tr p 125 I le Tr p G n Su I e 205 Pr o Leu Pr o Al a Tr p 285 Pr o Leu Thr Phe Tyr Leu Gy I e Ser Thr Lys 175 Leu Thr Val Sy Asn 255 l le Leu Leu Sy Al a SIe Asn Thr Phe SIe Lys Val 160 Leu Cys Hi s Leu Val 240 Tyr Sy Leu Arg Lys Lys Arg Thr Pro Thr y Lys lie Ala Arg Leu Phe n Ile Page 830 Gy 305 Tr p Pr o Asp SIle Leu 385 Ser Pr o Lys Leu Phe Ser Gy 355 Phe Ar g G u Thr Al a G u 325 Gy Ser SIle Al a Lys 405 Leu 295 Pr o Gy Al a Hi s Tyr 375 Leu Gy Phe Gene Sequence 24-2-06.txt 300 Phe Pro Phe Leu Ala Asn Ser 315 Arg G n Pro Trp Val Val Hi s 330 Arg Thr Gu IVet lie Arg IVet 345 350 Ala Pro Trp G n Val Trp Leu 360 365 Leu Ile Leu Phe Val Val Trp 380 lie Gy Pro Pro Gu Gu Gy 395 Pro Ala Thr Pro Ile y Ser 410 Thr Ala Ala Ala Pro Thr Thr 425 430 Al a Pr o 335 Thr Thr Val Al a Asp 415 Gy <210> 647 <211> 307 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(307) <223> RXA00379 <400> 647 cgtgctggat aaacagcacc gccccgcagc agtgttcttg tgtgttggat gttgcgttgc cattggtaga tgaggcctaa gta gcc caa agc atc ggc cag cag t Val Ala Gn Ser Ile G y Gn n F cca ctg ttc ctt ggc atc ctt gcc g Pro Leu Phe Leu Gy Ile Leu Ala A ttt gcc agc ccg tgt gtt gtg ccg t Phe Ala Ser Pro Cys Val Val Pro L 45 ctc gcc ggc gtg gtc ggt ggg gaa g Leu Ala y Val Val G y G y Gu 60 <210> 648 <211> 69 <212> PRT <213> Corynebacter i um gl ut ai cum gct Al a 15 gcg Al a gt g Val gaa Gu cgcgaagtca cctccaaaga atg tct gag att gtg IVet Ser G u lie Val 1 gtc gca gct tcc ggg Val Ala Ala Ser y gca ggt ctg gtg tct Ala y Leu Val Ser gga tat att tcc tac Sy Tyr lie Ser Tyr agc gct cat gcc acc Ser Ala His Ala Thr Page 831 Gene Sequence 24-2-06.txt <400> 648 Met Ser Ou I e Val Val Ala n Ser Ile Gy On n Phe Ala Asp 1 5 10 Val Al a Al a Ser G y Pro Leu Phe Leu G y Ile Leu Al a Ala Ala Leu 25 Ala G y Leu Val Ser Phe Ala Ser Pro Cys Val Val Pro Leu Val Pro 40 Sy Tyr lie Ser Tyr Leu Ala y Val Val Gy Gy G u Val G u Tyr 55 Ser Ala His Ala Thr <210> 649 <211> 362 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> <223> RXA00385 <400> 649 ctt ggc gga gtg ttc gct ctc ggt tgg acc cct tgt ttg ggc ccc acc 48 Leu Gy y Val Phe Ala Leu Gy Trp Thr Pro Cys Leu y Pro Thr 1 5 10 ctg gct gcg atc atc tcc att tct gca ggt act gaa ggc atg acc gct 96 Leu Ala Ala lie lie Ser Ile Ser Ala y Thr u Gy IVet Thr Ala 25 gcg cgt ggc gtg atc tta att gtg ggt tac tgc ctc gga ctg ggg ctg 144 Ala Arg y Val lie Leu Ile Val y Tyr Cys Leu y Leu y Leu 40 ccg ttc ctg ctg atc gcg ttg ggc tcc agc aag gca ctc acc gga gtc 192 Pro Phe Leu Leu lie Al a Leu y Ser Ser Lys Ala Leu Thr y Val 55 gag tgg ttg cgc aag cat tcc cgc acc ctg caa att atc ggc ggt gtg 240 Su Trp Leu Arg Lys His Ser Arg Thr Leu On lie lie G y G y Val 70 75 ttt ttg atc ttg gtc gga gta gcg ttg ctc tct ggc tca tgg gca att 288 Phe Leu Ile Leu Val G y Val Ala Leu Leu Ser Gy Ser Trp Ala lie 90 ttt atc aac tgg gtc cgt cag tgg acc gtt gaa tac ggc gca aca ctg 336 Phe Ile Asn Trp Val Arg G n Trp Thr Val G u Tyr y Ala Thr Leu 100 105 110 ctc tagaaaaaga cttttagtag gaa 362 Leu <210> 650 <211> 113 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 650 Page 832 Le u Le u Al a Pr o G31u Ph e Ph e Le u G31y Al a G31y Le u Le u I Ie As n Ph e li1e I Ie I Ie Ly s ValI ValI Al a Ser Le u Al a Hi s 70 G31y Ar g Le u I Ie I Ie Le u Ser ValI O n ene Sequence 24- 2- 06. txt Gy Trp Thr Pro Cys Leu Gy 10 Ser Al a G3 y Thr G3 u G3 y I'bt Val G31y Tyr Cys Leu G31y Leu 40 (31y Ser Ser Lys Ala Leu Thr Ar g Thr Leu O31n Ilie IIe G31y Al a Leu Leu Ser G31y Ser Tr p 90 Tr p Thr Val O31u Tyr G31y Al a 105 110 Pr o Thr G31y G31y G31y Al a Thr <210O> 651 <21 1> 901 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (901) <223> RXA01743 <400> 651 aggcaaagac tggaccggca accccgattg gt tcagat at gcccat gaca ccgct gcaat ttactgccgc tgccccaacc acaggtgaaa aggaataacc at g gat I'b t As p tt t Ph e g aa G31u g at As p g ac As p gcc Al a ct g Le u tt t Ph e tt c Ph e gcg Al a aat As n cct Pr o ct c Le u ct c Le u tt c Ph e ag g Ar g gt g ValI t gg Tr p ct t Le u gca Al a gt c ValI ac a Thr ct g Le u gca Al a t cg Ser tt t Ph e gga G31y gt g ValI 45 at c I Ie ac g Thr tt g Le u tt g Le u at t I Ie at c I Ie gt g ValI at g vb t at c I Ie gcg Al a gca Al a ac g Thr ggt G31y t cc Ser 80 cgc Ar g 1 gga G31y ccc Pr o at t G31y gga G31y gt g ValI t ac Tyr at c G31y gct Al a at g vb t gt g ValI ct c aat acc Leu Asn Thr t tt ct c ct c Phe Leu Leu at c ggt aaa Ile G3 y Lys cct gt c t gg Pr o Val Tr p t tg t tt gct Leu Phe Al a t at ct g ccg Tyr Leu Pr o ggc ct t gaa O3 y Leu G3 u 1 00 t gg cgc aag aaa gt c gat gat cct cgt t gg caa aag t gg t ct gac cgg Tr p Ar g Lys Lys Val Asp Asp Pr o Ar g Tr p O31n Lys Tr p Ser Asp Ar g Page 833 Gene Sequence 24-2-06.txt gcc Al a ttc Phe gat Asp 150 ggt Gy at c l Ie gct Al a ttg Leu gt g Val 230 gac Asp gt c Val ttt Phe 120 aat Asn gcg Al a ctt Leu ct g Leu gt a Val 200 gct Al a at c SIle gat Asp gca Al a ggt Gy ttg Leu gcc Al a ttc Phe 170 act Thr gca Al a at c l Ie gca Al a tta Leu 250 ct g Leu tct Ser cgt Ar g ctt Leu 155 acc Thr gct Al a ctt Leu gca Al a gcg Al a 235 agc Ser ttt Phe tgg Tr p ggc Gy 140 cct Pr o gca Al a ggt Gy ctt Leu t ac Tyr 220 gtt Val ttc Phe 110 cca Pr o ccc Pr o at g tet ttc Phe gt g Val 190 gcg Al a cgt Ar g ggt Gy t cc Ser ccg Pr o ct c Leu gt c Val gcc Al a 175 cgc Ar g gt g Val t cc Ser gga Gy act Thr 255 ct g Leu aag Lys aac Asn 160 ctt Leu acc Thr act Thr tgg Tr p gct Al a 240 t cc Ser tgg Tr p 130 gat Asp ttc Phe ggt Gy gcg Al a gga Gy 210 tgg Tr p gca Al a gct Al a Phe Gy Val I e Thr Leu Pr o gga Gy cac Hi s gcc Al a ct c Leu gcg Al a 195 cct Pr o at c SIle ct g Leu gt c Val ttc Phe acc Thr at t l Ie gca Al a 180 aag Lys ttc Phe ct c Leu at c SIle at c SIle 260 at c SIe at c SIe ctg Leu 165 ttc Phe gca Al a gtg Val gca Al a aaa Lys 245 ggt Gy <210> 652 <211> 267 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 652 lIet Asp Leu Asn Thr Phe Trp Phe lie 1 5 Sy Tyr Phe Leu Leu G u y Phe Asp Pro lie lie y Lys Asp Ser Ala Ala 40 lie Gy Pro Val Trp Asp Gy Asn Gu 55 Sy Ala Leu Phe Ala Al a Phe Pro G u 70 Gy IVet Tyr Leu Pro Leu Phe Leu Val Val Val y Leu G u Trp Arg Lys Lys Leu Il e Al a 10 Phe G y Val Arg Asn Thr Val Trp Leu Trp Tyr Al a 75 Leu Val Ser 90 Val Asp Asp Page 834 Phe Leu Ar g Al a Phe let Tr p Al a Al a Thr Gy Ser Ar g G n Lys Mebt Al a 145 Val Hi s Asp Gy Ser 225 Phe Val Ser 115 Gy Hi s Al a Leu Al a 195 Pr o SIle Leu Val Ar g I e I e Leu 165 Phe Al a Val Al a Lys 245 Gy Al a Phe Asp 150 Gy I e Al a Leu Val 230 Asp Val l Ie Al a 135 Al a Al a Ar g Pr o Tr p 215 Leu Ar g Val Gene Sequence 24-2-06.txt 105 110 Phe lie Gy Ser Trp Thr Pro 120 125 Asn Ile Leu Arg G y ebt Pro 140 Ala Ala Ala Leu Pro Gy Mbet 155 Leu Ala Phe Thr Ala Leu Phe 170 Leu Lys Thr Ala Gy Arg Val 185 190 Val Val Ala Leu Leu Ala Ala 200 205 Ala Ala Ile Ala Tyr y Arg 220 Ile Ile Ala Ala Val Leu y 235 Asp G y Leu Ser Phe Leu Ser 250 Ala Leu Leu Phe 265 Pr o Leu Val Al a 175 Ar g Val Ser Gy Thr 255 <210> 653 <211> 1779 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1756) <223> RXN02480 <400> 653 acctatgacc gctgtggcgc ctagggtcga cgggcacgtc gacaggccat gcacgcaagg gcagcaaagc atggttaatg cac Hi s ttc Phe cct Pr o at g et ggt Gy cag G n ggt Gy ct g Leu gga Gy gct Al a ggc Gy ttg Leu ttc Phe gt c Val t ac Tyr at g et gcc Al a tct Ser ct g Leu ct g Leu t ac Tyr ct g Leu aat Asn 45 ct g Leu cca Pr o at c SIle ctt Leu gag G u ct g Leu ctt Leu att atg I e bet 15 at c cga li e Ar g cag ttc G n Phe tac gga Tyr G y cag at c Gn lie Page 835 gcccctcaga ggcccgagcc atg acc acc acc gac et Thr Thr Thr Asp 1 tcc ttc agc ttc ttc Ser Phe Ser Phe Phe gcg gag ctt ttc acc Ala G u Leu Phe Thr aac cag ctg ttc acc Asn G n Leu Phe Thr act cca att gtt tgg Thr Pro Ile Val Trp ggt gcg cct gac gta G y Ala Pro Asp Val Gene Sequence 24-2-06.txt gct Al a ggt Gy ttc Phe ggc Gy ggc Gy 150 cgc Ar g at c SIle gct Al a t ac Tyr ttc Phe 230 at t SIle t ac Tyr gct Al a ttc Phe ttc Phe 310 acc Thr ggt Gy cca Pr o gcg Al a tgg Tr p 120 ggc Gy gtt Val cct Pr o gtt Val gca Al a 200 cca Pr o gga Gy tct Ser ggc Gy tgg Tr p 280 t cc Ser aac Asn at g Met acc Thr ttg Leu ct g Leu at g let gac Asp t cc Ser at g Met 170 t cc Ser ggt Gy aac Asn cct Pr o at c SIle 250 at c SIle cac Hi s at g let gtt Val tgg Tr p 330 at t SIle aat Asn acc Thr tac Ty r at g bet gca Al a 155 acc Thr gtt Val gtt Val ggc Gy gag Gu 235 at t SIle tt c Phe cac Hi s acg Thr gga Gy 315 t ct Ser at g bet gct Al a ggc Gy t cc Ser tgg Tr p 140 at t l Ie at g et ctt Leu ct g Leu ggc Gy 220 gtt Val cct Pr o gca Al a at g tet ttc Phe 300 acc Thr gtt Val ct g Leu ttc ggc Phe G y ttc ctg Phe Leu 110 cca ct g Pro Leu 125 att gtc Ile Val aac at g Asn IVet ttc cgt Phe Arg gct ctg Al a Leu 190 tat gac Tyr Asp 205 t cc ctc Ser Leu tac gtt Tyr Val gtg ttc Val Phe acc ttg Thr Leu 270 ttc gtt Phe Val 285 ctg att Leu II e atg tgg let Trp ggc ttc G y Phe gcg tcc Al a Ser tgg Tr p ccg Pr o gac Asp gt c Val acc Thr 160 cct Pr o at c SIle aag Lys tgg Tr p gcg Al a 240 cgt Ar g at t SIle ggc Gy gtt Val ggt Gy 320 gct Al a acc Thr ggt Gy at t SIle 130 gca Al a at c SIle ttc Phe cca Pr o ggt Gy 210 cac Hi s ccg Pr o cca Pr o gca Al a gtt Val 290 acc Thr at c SIle ttc Phe acc Thr gct Al a 115 cac Hi s act Thr ct c Leu acc Thr ct g Leu 195 gga Gy ct g Leu ttc Phe at g Net ct g Leu 275 ttg Leu ggc Gy act Thr ct c Leu gt c Val 100 gcc Al a t cc Ser ggt Gy tgc Cys tgg Tr p 180 ct g Leu cac Hi s ttc Phe ttc Phe ttc Phe 260 t cc Ser ctt Leu gtt Val tgg Tr p ttc Phe 340 ggt Gy gac Asp cca Pr o at t SIe ct c Leu 165 aat Asn ct c Leu ct g Leu tgg Tr p ggc Gy 245 ggt Gy at g et ccg Pr o aag Lys gaa G u 325 ggt Gy 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 cca cca ct g Pro Pro Leu Page 836 gac ttc cac ttg Asp Phe His Leu Gene Sequence 24-2-06.txt 345 350 355 gct gac tcc tac ttc ctg atc gcg cac ttc cac tac acc ctc ttc ggt 1219 Ala Asp Ser Tyr Phe Leu lie Ala His Phe His Tyr Thr Leu Phe G y 360 365 370 acc gtg gtg ttc gca tcg tgt gca ggc gtt tac ttc tgg ttc ccg aag 1267 Thr Val Val Phe Ala Ser Cys Ala y Val Tyr Phe Trp Phe Pro Lys 375 380 385 atg act ggc cgc atg atg gac gag cgt ctt ggc aag atc cac ttc tgg 1315 IVet Thr G y Arg IVet IVet Asp G u Arg Leu G y Lys lie His Phe Trp 390 395 400 405 ttg acc ttc gtc ggt ttc cac gga acc ttc ctc atc cag cac tgg gtg 1363 Leu Thr Phe Val G y Phe His G y Thr Phe Leu lie G n His Trp Val 410 415 420 ggc aac atg ggt atg cca cgt cgt tac gct gac tac ctg gat tct gat 1411 G y Asn IVet G y Iet Pro Arg Arg Tyr Al a Asp Tyr Leu Asp Ser Asp 425 430 435 ggt ttc acc atc tac aac cag atc tcc acc gtg ttc tac ttc ctg ctt 1459 G y Phe Thr Ile Tyr Asn G n Ile Ser Thr Val Phe Tyr Phe Leu Leu 440 445 450 ggc ctg tct gtc att cca ttc atc tgg aac gtc ttc aag tcc tgg cgc 1507 Sy Leu Ser Val Ile Pro Phe lie Trp Asn Val Phe Lys Ser Trp Arg 455 460 465 tac ggt gag ctc gtt acc gtt gat gat cct tgg ggt tac ggc aac tcc 1555 Tyr Gy G u Leu Val Thr Val Asp Asp Pro Trp y Tyr y Asn Ser 470 475 480 485 ctg gag tgg gca acc tcc tgc cct cct cct cgc cac aac ttc gca tcc 1603 Leu G u Trp Ala Thr Ser Cys Pro Pro Pro Arg His Asn Phe Ala Ser 490 495 500 ttg cct cgt atc cgc tcc gag cgc cct gcg ttc gag ctg cac tac ccg 1651 Leu Pro Arg lie Arg Ser G u Arg Pro Ala Phe G u Leu His Tyr Pro 505 510 515 cac atg att gaa cgc atg cgc gca gag gca cac act gga cat cac gat 1699 His IVet lie G u Arg IVet Arg Ala G u Ala His Thr G y His His Asp 520 525 530 gat att aat gct cca gaa ttg ggt acc gcc cca gcc ctt gca tct gac 1747 Asp Ile Asn Ala Pro G u Leu y Thr Ala Pro Ala Leu Ala Ser Asp 535 540 545 tcc agc cgc taaaagcgtc tgatttaagt cgg 1779 Ser Ser Arg 550 <210> 654 <211> 552 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 654 IVet Thr Thr Thr Asp His Lys n Leu G y IIe Iet Tyr lie lie Iet 1 5 10 Ser Phe Ser Phe Phe Phe Leu Gy y Leu IVet Ala Leu Leu lie Arg 25 Ala G u Leu Phe Thr Pro y Leu G n Phe Leu Ser Asn G u G n Phe Page 837 ene Sequence 24- 2- 06. txt As n Th r G31y I Ie G31y Al a G31y 145 Th r I Ie Ph e Le u O31n 225 Le u Ly s G31y Al a Pr o 305 Hi s Th r Le u Ty r Ph e 385 Le u I Ie Pr o Th r Al a 115 Hi s Th r Le u Th r Le u 1 95 G31y Le u Ph e vb t Le u 275 Le u G31y Th r Le u Ph e 355 Le u Ph e Th r Tr p ValI G31y As p Pr o I Ie Le u 1 65 As n Le u Le u Tr p G31y 245 G31y vb t Pr a Ly s G31u 325 G31y Le u G31y Ly s vb t (31y 70 Al a G31y Ph e G31y G31y 150 Ar g I Ie Al a Ty r Ph e 230 li1e Ty r Al a Ph e Ph e 310 Th r G31y Al a Th r vb t 390 Hi s 03 y Th r Phe Al a As n Phe Pr o Ar g Val Al a I'b t 1 G31y Tr p Thr 120 Leu 03 y Ser 135 Ser Val Al a Al a Pr o G31y Phe Val Val 1 Al a Al a Leu 200 As p Pr o Al a 215 Phe 03 y Hi s Val Ser G31u Val G31y Leu 265 Val Tr p Al a 280 Phe Ser Phe 295 Phe As n Tr p Pr o I'b t IIe Leu Thr 03 y 345 As p Ser Ty r 360 Val Val Phe 375 Thr G31y Ar g Leu Leu Tyr 03 y Val I'bt Leu Ty r Val Leu 75 Leu Asn Al a 90 Leu Thr (3 y I'b t Tyr Ser Asp I'b t Tr p 140 Ser Al a Il e 1 55 1 70 Ser Val Leu G31y Val Leu Asn (1y (1y 220 Pro 031u Val 235 Ilie Ilie Pr o 250 Il e Phe Al a Hi s Hi s I'b t I'b t Thr Phe 300 Val G31y Thr 315 Tr p Ser Val 330 Ilie I'b t Leu Phe Leu Il e Al a Ser Cys 380 Pr o Ph e Ph e Pr o 125 li1e As n Ph e Al a Tyr 205 Ser Tyr ValI Thr Ph e 285 Le u vb t G31y Al a Al a 365 Al a (31n Ph e Thr Ser G31y Le u vb t 1 Le u Ar g Le u Le u Ser 255 Ser Thr Ser Ly s vb t 335 Pr o Ph e ValI lIe Tr p Pr o As p ValI Thr 1 Pr o I Ie Ly s Tr p Al a 240 Ar g I Ie G31y ValI G31y 320 Al a Pr o Hi s Ty r G31y 400 I'b t I'b t As p 031u Ar g Le u 395 Page 838 Gene Sequence 24- Lys lie His Phe Trp Leu Thr Phe Val G y Phe 405 410 I Ie G n His Trp Val G y Asn Met G y Met Pro 420 425 Tyr Leu Asp Ser Asp G y Phe Thr Ile Tyr Asn 435 440 Phe Tyr Phe Leu Leu G y Leu Ser Val Ile Pro 450 455 Phe Lys Ser Trp Arg Tyr Gy G u Leu Val Thr 465 470 475 Sy Tyr y Asn Ser Leu G u Trp Ala Thr Ser 485 490 His Asn Phe Ala Ser Leu Pro Arg lie Arg Ser 500 505 Su Leu His Tyr Pro His IVet Ile u Arg Iet 515 520 Thr G y His His Asp Asp IIe Asn Al a Pro G u 530 535 Ala Leu Ala Ser Asp Ser Ser Arg 545 550 <210> 655 <211> 385 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(385) <223> FRXA01919 <220> <221> i sc feature <222> 345 <223> n a,c,g,or t <220> <221> VARIANT <222> 82 <223> Xaa IVet, Thr, Lys, or Arg <400> 655 acctatgacc gctgtggcgc ctagggtcga cgggcacgtc gacaggccat gcacgcaagg gcagcaaagc atggttaatg cac aag cag ctg ggc att atg tac atc att atg His Lys G n Leu y I I e IVet Tyr lie lie IVet 15 ttc ctc ggt ggc ttg atg gcc ctg ctt atc cga Phe Leu G y G y Leu IVet Al a Leu Leu lie Arg 30 cct ggt ctg cag ttc ctg tct aat gag cag ttc Pro y Leu G n Phe Leu Ser Asn G u G n Phe Page 839 2-06. txt Hi s G y Thr Arg Arg Tyr 430 G n Ile Ser 445 Phe Il e Tr p 460 Val Asp Asp Cys Pro Pro G u Arg Pro 510 Ar g Al a G u 525 Leu G y Thr 540 Phe 415 Al a Thr Asn Pr o Pr o 495 Al a Al a Al a gcccctcaga ggcccgagcc atg acc acc acc gac Vet Thr Thr Thr Asp 1 tcc ttc agc ttc ttt Ser Phe Ser Phe Phe gcg gag ctt ttc acc Ala G u Leu Phe Thr aac cag ctg ttc acc Asn G n Leu Phe Thr Gene Sequence 24- 2- 06. txt 45 atg cac gga act gtc atg ctg ctg ctg tac gga act cca att gtt tgg IVet His G y Thr Val IVet Leu Leu Leu Tyr G y Thr Pro I I e Val Trp 60 ggt ttt gct aac tac gtc ctg cca ctt cca acc acc ang caa gcc ttc Sy Phe Ala Asn Tyr Val Leu Pro Leu Pro Thr Thr Xaa G n Ala Phe 75 80 tct aaa acc cgg gtg aac tct ccc agg gag Ser Lys Thr Arg Val Asn Ser Pro Arg G u <210> 656 <211> <212> PRT <213> Corynebacter i um gl ut am cum <220> <221> VARIANT <222> 82 <223> Xaa IVet, Thr, Lys, or Arg <400> 656 IVet Thr Thr Thr Asp His Lys G n Leu 1 5 Ser Phe Ser Phe Phe Phe Leu G y G y Ala G u Leu Phe Thr Pro y Leu G n 40 Asn G n Leu Phe Thr IVet His G y Thr 55 Thr Pro Ile Val Trp G y Phe Ala Asn 70 Thr Xaa G n Ala Phe Ser Lys Thr Arg SIle et Leu et Val Asn vet Al a Ser Leu Leu Ser Tyr Leu Asn Leu Pr o Pr o I e I e G n Tyr Pr o Su <210> 657 <211> 972 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(949) <223> FRXA02480 <400> 657 tacgttctgg cgctgccgtt cttcggcatt gtttctgaga tcat t cctgt gttctcccgt aagccaatgt tcgggttacg tcggcctgat cttcgcaacc ttg tcc att ggt gca Leu Ser lie Gy Ala 1 ctg tcc atg gct gtg tgg gct cac cac atg ttc gtt act ggc gca gtt Leu Ser et Ala Val Trp Ala His His et Phe Val Thr G y Ala Val 15 ttg ctt ccg ttc ttc tcc ttc atg acg ttc ctg att tcg gtt cct acc Leu Leu Pro Phe Phe Ser Phe IVet Thr Phe Leu Ile Ser Val Pro Thr Page 840 Gene Sequence 24-2-06.txt ggc gtt aag G y Val Lys act tgg gaa Thr Trp G u ctc ttc ggt Leu Phe G y ttc cac ttg Phe His Leu ctc ttc ggt Leu Phe G y ttc ccg aag Phe Pro Lys 120 cac ttc tgg Hi s Phe Trp 135 cac tgg gt g Hi s Trp Val 150 gat t ct gat Asp Ser Asp ttc ctg ctt Phe Leu Leu tcc tgg cgc Ser Trp Ar g 200 ggc aac t cc G y Asn Ser 215 ttc gca tcc Phe Al a Ser 230 cac tac ccg His Tyr Pro cat cac gat His His Asp gca t ct gac Al a Ser Asp 280 <210> 658 ttc Phe cca Pr o ct g Leu gac Asp gt g Val act Thr acc Thr aac Asn ttc Phe 170 ct g Leu ggt Gy gag G u cct Pr o at g Met 250 at t SIle tgg Tr p at c SIle ggc Gy t ac Tyr ttc Phe cgc Ar g gt c Val 140 ggt Gy at c SIle gt c Val ct c Leu gca Al a 220 at c SIle gaa G u gct Al a gtt Val 45 tgg Tr p at t SIle ttc Phe gca Al a at g Met 125 ggt Gy at g Met t ac Tyr at t SIle gtt Val 205 acc Thr cgc Ar g cgc Ar g cca Pr o gga Gy tct Ser at g et ct g Leu tcg Ser 110 at g tet ttc Phe cca Pr o aac Asn cca Pr o 190 acc Thr t cc Ser t cc Ser at g Net gaa G u 270 at g bet ggc Gy gcg Al a gcg Al a gca Al a gag G u gga Gy cgt Ar g 160 at c SIle at c SIle gat Asp cct Pr o cgc Ar g 240 gca Al a ggt Gy aag Lys at g Net cca Pr o ttc Phe gtt Val ctt Leu 130 ttc Phe gct Al a acc Thr aac Asn cct Pr o 210 cct Pr o gcg Al a gca Al a gcc Al a ggt Gy gct Al a cca Pr o cac Hi s t ac Tyr 115 ggc Gy ct c Leu gac Asp gt g Val gt c Val 195 tgg Tr p cgc Ar g ttc Phe cac Hi s cca Pr o 275 cac Hi s acc Thr ctg Leu t ac Tyr 100 ttc Phe aag Lys at c SIe t ac Tyr ttc Phe 180 ttc Phe ggt Gy cac Hi s gag G u act Thr 260 gcc Al a tcc agc cgc Ser Ser Arg taaaagcgtc tgatttaagt cgg Page 841 21> 23 ene Sequence 24- 2- 06. txt <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm curn <400> 658 Leu Ser I Ie ValI Tr p Ph e Ser Hi s G31y Ar g Th r 145 Ty r Ser Tr p As p Pr o 225 Pr o (31u Th r G31y ValI G31y A a Pr o Hi s Ty r 115 G31y Le u As p ValI ValI 1 95 Tr p Ar g Ph e Hi s Pr o 275 O3 y A a Leu A a Val Leu Pr o Thr G31y Hi s I I e Thr Thr Phe Leu 70 Leu Asp Phe Tyr Thr Leu 1 00 Phe Trp Phe Lys IlIe Hi s Ile G3 n Hi s 150 Tyr Leu Asp 1 65 Phe Tyr Phe 1 80 Phe Lys Ser a3 y Tyr G3 y Hi s Asn Phe 230 G3 u Leu Hi s 245 Thr a3 y Hi s Ser Le u ValI Tr p Ph e Hi s Ph e Pr o Ph e 135 Tr p Ser Le u Tr p As n 215 A a Tyr Hi s A a Val 10 Phe Phe Phe Phe Thr Pr o a3 y Leu A a As p 90 Thr Val 1 I'b t Thr Leu Thr 31y As n a3 y Phe 1 70 a3 y Leu 1 Ty r a3 y Leu a3 u Leu Pro Hi s I'b t 250 As p I I e 265 Trp Ala His His N/t Phe Ser As n vb t Thr Ser ValI a31y Ph e vb t 1 Thr Ser a31u Tr p Ar g 235 li1e As n vb t ValI Tr p I Ie Ph e A a vb t 125 a31y vb t Tyr I Ie ValI 205 Thr Ar g Ar g Pr a Ph e Thr ValI Le u I Ie cys As p Hi s Ar g a31n 1 Ph e ValI cys a31u Ar g 255 Le u A a Leu A a Ser Ser Ser Ar g <210O> 659 <21 1> 735 <212> DNA <21 3> <220> <221 <222> Cor ynebact er i urn gi ut anm curn ODS 101). (712) Page 842 Gene Sequence 24-2-06.txt <223> FRXA02481 <400> 659 tgctaactac gtcctgccac ttcagatcgg tgcgcctgac tgctttcggc ttctgaatca ccaccgtcgg tggtgtcgcg gtagctttcc cacgttgaaa atg ctg acc ggc ttc 111 Met Leu Thr G y Phe ct a Leu tgt Cys tcg Ser at g et cgt Ar g ct g Leu gac Asp ct c Leu gtt Val ttc Phe 150 ctt Leu cgt Ar g gat Asp ccg Pr o acg Thr gt c Val acc Thr cct Pr o at c SIle aag Lys tgg Tr p 120 gcg Al a cgt Ar g cat Hi s tgg Tr p ggt Sy 200 ggt Sy ttc Phe gca Al a at c SIle ttc Phe cca Pr o ggt Gy cac Hi s ccg Pr o cca Pr o tgc Cys 170 agt Ser gct Al a act Thr act Thr ct c Leu ace Thr 75 ct g Leu gga Gy ct g Leu tt c Phe at g bet 155 act Thr ttt Phe gcc gac ttg Al a Asp Leu ccc cag gcc Pro G n Ala ggt att ggc G y lie G y 45 t gc ct c cgc Cys Leu Arg tgg aat at c Trp Asn lle ct g ct c gct Leu Leu Al a cac ct g tac Hi s Leu Tyr 110 ttc tgg ttc Phe Trp Phe 125 ttc ggc att Phe G y Ile 140 ttc ggg tta Phe G y Leu gt c cat ggc Val His y gct tcc gtt Al a Ser Val 190 ggt G y 15 ttg Leu t cc Ser gca Al a ttc Phe gct Al a 95 gat Asp ttc Phe gtt Val cgt Ar g tgt Cys 175 ctt Leu gga G y gtt Val gtt Val cct Pr o gtt Val gca Al a cca Pr o gga Gy tct Ser cgg Ar g 160 gt g Val ct c Leu 1 cca Pr o ct a Leu gct Al a ggt G y gtt Val ct g Leu gct Al a cac Hi s gag Gu 145 cct Pr o ggc G y ctt Leu tgt Cys aca Thr t cc Ser at g et t cc Ser ggt Gy aac Asn cct Pr o 130 at c SIle gat Asp t ca Ser cat Hi s act Thr tgt Cys gca Al a acc Thr gtt Val gtt Val ggc Gy 115 gag Gu at t lile ctt Leu cca Pr o gac Asp 195 ccc Pr o gga G y att SIe at g bet ctt Leu ctg Leu 100 ggc Gy gtt Val cct Pr o cgc Ar g cat Hi s 180 gtt Val tcc tac cgg cgt Ser Tyr Arg Arg taagttcttc aactgggttg gaa <210> 660 <211> 204 <212> PRT <213> Corynebacteri um gl utami cum <400> 660 Page 843 I'bt Leu Thr 03 y Phe Leu Thr 1 5 ene Sequence 24- 2- 06. txt Pro Oy Oy Ala Ala Asp Leu G31y (1y Pr o Le u Al a G31y ValI Le u Al a Hi s (31u 145 Pr o G31y Le u Thr cys Al a Thr ValI ValI G31y 115 (31u I Ie Le u Pr o As p 1 95 Hi s Le u As n Ph e Al a Tyr Ser Tyr ValI As n 1 65 ValI Pr o Le u G31y Le u vb t Le u Ar g Le u Le u 135 Ser ValI Tyr Ph e Thr ValI 40 Thr Pr o I Ie Ly s Tr p 120 Al a Ar g Hi s Tr p G31y 200 O31n G31y Thr I Ie Ph e Le u 1 05 O31n Le u Ly s Tr p Ar g 1 85 Ser Ph e Al a I Ie Ph e Pr o 90 G31y Hi s Pr o Pr o cys 1 70 Ser Tyr Thr Thr Le u Thr Le u G31y Le u Ph e vb t 1 Thr Ph e Ar g O31n lIe Le u As n Le u Le u Tr p 125 G31y G31y Hi s Ser Al a G31y Ar g I Ie Al a Ty r 110 Ph e I Ie Le u G31y ValI 1 Le u Ser Al a Ph e Al a As p Ph e ValI Ar g cys 1 Le u <210O> 661 <21 1> 1200 <21 2> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CDS <222> (1177) <223> RXA02140 <400> 661 aacacaagat at ggaat cgg ct ggcaaat a ggc aggt ct ctgg t tt t tgt gtg gacaggaagg cag t at t ct g cgaagat aga aat gaccgt a ;aacacac gtg gaa cag caa aat 115 Val O31u (1n 1n Asn 1 ct t ggc ggt gt c t tg ggc t ta 163 Leu G31y G31y Val Leu G31y Leu 15 gt c gcc cct cct ggc ggt gt g 211 Val Al a Pr o Pr o G31y G31y Val cct gat ggc at t acc cct gaa 259 Pr o Asp (3y Ilie Thr Pr o (3u Page 844 aag cgt ggt Lys Ar g G31y ggt ggc ct c 03 y 03 y Leu ct t gga gat Leu (31 y Asp tta aag cgc aag gcc ctg Leu Lys Arg Lys Ala Leu gcc atg gca ggc tgt gaa Al a I'b t Al a 03 y Cy s 03 u 30 t tc ct a cgt at g ggt t gg Phe Leu Ar g N/t (3y Tr p 45 Gene Sequence 24-2-06.txt gca Al a at c SIle tgg Tr p cag G n at c SIle aag Lys gct Al a 150 ct g Leu gca Al a cac Hi s gaa G u aac Asn 230 ttc Phe gag G u gaa G u gca Al a gag G u 310 gcc Al a ggc G y gca Al a cag G n at t SIle 120 acc Thr cag G n cct Pr o gcc Al a aac Asn 200 ct c Leu cca Pr o gtt Val aac Asn gca Al a 280 at g Met at c SIle ggt aac ttc tgg Sy Asn Phe Trp atc atg tgg ggt Ilie Vet Trp y 75 agg gct gaa aag Arg Ala G u Lys aac gtt cca ctt Asn Val Pro Leu atg gtg ctg ttc et Val Leu Phe 125 ctg gat aag aac Leu Asp Lys Asn 140 aac tgg aag ttc Asn Trp Lys Phe 155 gga cag gat tac G y G n Asp Tyr 170 gct tcc aag aag Ala Ser Lys Lys aag tct gac gtt Lys Ser Asp Val 205 acc act gat gaa Thr Thr Asp G u 220 gag ttc aac ctc G u Phe Asn Leu 235 gag ttc ctc ttc G u Phe Leu Phe 250 tcc cag cgt gtc Ser G n Arg Val gtt ggt cgc tgt Val G y Arg Cys 285 ttc gag ctt cgt Phe G u Leu Ar g 300 ttc cgt gac tcc Phe Arg Asp Ser 315 t ca tgg gt c Ser Trp Val tgg gtt gct gcc tgg Al a Al a Trp ttc Phe ggc G y 95 ct c Leu ttc Phe gag G u t ac Tyr gga G y 175 cct Pr o t ac Tyr cca Pr o tct Ser cga Ar g 255 cag G n gaa G u gt c Val cca Pr o ct c Leu 80 gag G u gtt Val acc Thr gtt Val t cc Ser 160 agc Ser tct Ser ctt Leu gt g Val gct Al a 240 gat Asp at t SIle at g et gat Asp gac Asp 320 Irp acc Thr ggt G y ct g Leu gtt Val acc Thr 145 gaa G u gac Asp gga Gy gag G u at g let 225 gac Asp gct Al a gaa G u tgc Cys cgc Ar g 305 gca Al a val gcc Al a gaa G u acg Thr caa G n 130 gt g Val at t SIle ccg Pr o gat Asp ttc Phe 210 gtt Val gtt Val t ac Tyr gag G u ggt G y 290 gat Asp acc Thr at c l Ie ttc Phe at c SIle 115 act Thr gac Asp gat Asp gag G u aac Asn 195 aac Asn ctt Leu gca Al a gca Al a at c SIle 275 act Thr t cc Ser aac Asn ttt Phe cct Pr o 100 gtt Val cag G n gt c Val ggc G y cgt Ar g 180 cca Pr o cga Ar g cct Pr o cac Hi s cac Hi s 260 act Thr t ac Tyr ttc Phe gct Al a gcc Al a aag Lys ccg Pr o gac Asp acc Thr t ca Ser 165 cag G n at t SIe at t SIe gt g Val t cc Ser 245 cct Pr o gag G u cac Hi s gct Al a cag G n 325 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 Page 845 Gene Sequence 24- 2- 06. txt gca ctt gag cac at t ggt caa gct cct t ac gct act t cc act agc cca Ala Leu G u His lie G y n Ala Pro Tyr Ala Thr Ser Thr Ser Pro 330 335 340 ttc gtt tcc gat cgc acc gca acc cgc gac ggc gaa aac act cag agc Phe Val Ser Asp Arg Thr Ala Thr Arg Asp Gy G u Asn Thr G n Ser 345 350 355 aac gct taagaaggag tggcgaaaaa atg Asn Ala <210> 662 <211> 359 <212> PRT <213> Cor ynebact eri um gl ut ai cum 1123 1171 1200 <400> 662 Val Gu n 1 G y Val Leu Pro Pro G y G y I e Thr Trp Val Al a Thr Ala li e G y G u Phe Leu Thr II e 115 Val G n Thr 130 Thr Val Asp 145 G u Ile Asp Asp Pro u G y Asp Asn 195 G u Phe Asn 210 let Val Leu 225 Asp Val Al a Sn Asn 5 G y Leu G y Val Pro G u Al a Tr p Phe Al a Pro Lys 100 Val Pr o Sn Asp Val Thr G y Ser 165 Arg G n 180 Pro Ile Arg Ile Pro Val Hi s Ser 245 Lys Arg y Leu Sy Leu Al a SIle 70 Tr p G n SIle Lys Al a 150 Leu Al a Hi s G u Asn 230 Phe Sy Sy Val I le Sy Leu SIle Val 135 Tyr Al a Al a Sy Thr 215 Thr Tr p Leu Asp 40 Al a Sy Al a G n SIle 120 Thr G n Pr o Al a Asn 200 Leu Pr o Val Al a 25 Phe let SIle Lys Tyr 105 Val Al a Tr p Sy G u 185 Ser Gy I e Pr o Ar g Al a Ar g Asn bet 75 Al a Val Val Asp Tr p 155 G n Ser Ser Thr Phe 235 Al a Cys Sy Tr p Sy Lys Leu Phe 125 Asn Phe Tyr Lys Val 205 G u Leu Leu G u Tr p Ser Leu Ar g G u 110 Phe Pr o Sy G n Asp 190 Ser SIle Al a Leu G y Val Ala Pro Asp Trp Val Phe Leu G y G u Leu Val Phe Thr G u Val Tyr Ser 160 G y Ser 175 Pro Ser Tyr Leu Pro Val Ser Al a 240 Arg Asp 255 G u Phe Leu Phe Lys 250 Page 846 A a O31u Cys Ar g 305 A a Thr aOu A a I Ie 275 Thr Ser As n Thr Thr 355 Pr o O31u Hi s A a O31n 325 Pr o Ser O31u O31u A a O31u 310 A a Ph e As n ene Sequence 24- 2- 06. txt Asn Lys Ser O31n Ar g Val Phe 265 270 Ala Phe Val G31y Ar g Cys Ala 280 285 I'b t Asn Phe O31u Leu Ar g Val 300 Ilie Ser Phe Ar g Asp Ser Asn 315 (3 u Hi s Ile 03 y O31n A a Pr o 330 Ser Asp Ar g Thr A a Thr Ar g 345 350 O31n O31u ValI Pr o Ty r 335 As p <210O> 663 <21 1> 774 <212> DNA <213> Cor ynebact er i urn gI ut ari cumn <220> <221 ODS <222> (751) <223> RXA02142 <400> 663 acgaaaagt t cccggaaggt cgat tgaaaa gt t tgcgaat aagccgagt t caaact t tca at tgaaacgg ggggct tgaa t gggggaaaa t tcgcat caa c aa O31n at g vb t agt Ser ggg G31y gga G31y t tg Le u gt t ValI gcc A a gca A a ggc G31y t tc Ph e t gg Tr p ac g Thr gcg A a ct a Le u c aa O31n at t I Ie at g vb t gag O31u at t I Ie g aa O31u ag c Ser gt t ValI t tc Ph e t tc Ph e ac a Thr gt g ValI ggt O31y gt g ValI gcg A a ct g Le u 45 gt g ValI g at As p t ct Ser g ac As p ac g Thr gca A a t ct Ser t cc Ser c ac Hi s t cc Ser gt t ValI 110 gca A a aac As n g aa O31u gcg A a aac As n gt g ValI ggc O31y gt g ValI 1 gt t ValI cgt Ar g t ta Le u aac As n gt g ValI act Thr ct c Le u gga O31y ccg Pr a at g vb t gga O31y ccc Pr a t gc Cys cgc Ar g ac a Thr at g vb t tt c Ph e gca A a gca A a tt c Ph e 1 00 t gg Tr p act t tg gcc Thr Leu A a t tg gt c acg at t at c ct c gga t ca at c t tc gt g at c gga cag ggc t ac Page 847 Gene Leu Val Thr Ile Ile Leu Gy Ser I 120 125 gag tac atc act ctc gt a ggt cac g Su Tyr l e Thr Leu Val Gy His C 135 140 tac gga tcg gca ttc ttt att aca a Tyr G y Ser Al a Phe Phe I e Thr T 150 155 atc gcg ggt gtt atg gcc ttc gtt g Ile Ala y Val ebt Ala Phe Val 170 tcg aag ttc act ccg gca cag gca a Ser Lys Phe Thr Pro Al a n Ala T 185 1 tac tgg cac ttc gtt gac gtg gtc t Tyr Trp His Phe Val Asp Val Val T 200 205 tac ttc att cag taggcagtaa ggaatc Tyr Phe Ile n 215 <210> 664 <211> 217 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 664 Val Thr Leu Ala Asn G n Thr Ala I 1 5 Val Gy Asn Thr Gy et Ala Ala F Arg Pro Asn IVet Val Ser Val Gy T 40 Leu IVet Phe Phe Ala G y Leu Phe A Asn y Leu Ala Asn G y Ser Trp C 70 Val Pro Tyr Ala Leu Leu Ile Thr Thr Cys G n Phe y Val Phe Ala A 100 1 Leu Arg Lys Trp Phe Leu Val Thr I 115 120 lie y n y Tyr u Tyr Ile T 130 135 lie n Ser Ser Val Tyr y Ser A 145 150 His Ala Leu His Val Ile Ala Gy 165 Sequence 24-2-06. t xt le Phe Val Ile y n 130 ga ctt aca atc cag agc 3y Leu Thr Ile n Ser 145 cc ggt ttc cac gca ctg hr G y Phe Hi s Al a Leu 160 itg gtt ctt atg aga atc (al Val Leu IVet Arg Ile 175 cc gca gca atg gtt gtg hr Ala Ala Net Val Val 90 195 gg atc ggc ctc ttc atc rp Ile y Leu Phe Ile 210 ct ca acg Sy agt Ser cac Hi s cat Hi s 180 tct Ser act Thr le r o 25 Fhr 1 a ay (al 1 a 05 le "hr 1 a /al Asp Ar g Val Tyr G n Leu Ar g Leu Val Phe 155 Al a Val Al a Leu Val Asp Ser Asp Ser 125 Hi s Thr Val Thr Al a Ser Ser Hi s Ser Val 110 SIle Sy Thr Val Ser Leu G n Ar g Leu Ser Tyr Phe Leu Sy Val 175 IVet Arg Ile His Lys Ser Lys Phe Thr Pro Ala n Ala Thr Ala Ala Page 848 Gene Sequence 24-2-06.txt 180 185 190 Met Val Val Ser Tyr Tyr Trp His Phe Val Asp Val Val Trp IIe y 195 200 205 Leu Phe Ile Thr Ile Tyr Phe Ile G n 210 215 <210> 665 <211> 1347 <212> DNA <213> Corynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1324) <223> RXA02144 <400> 665 actcactcgg tagcttgggc ccagtggctg agggtctgtt catgtgggta ttcggcatct tggtcctcgt ggccgccgct atgtggat t g gatcacgttc gac Asp ctt Leu aag Lys cgt Ar g ttc Phe gat Asp act Thr gt c Val ggt Gy gac Asp 150 ctt Leu cag G n cga Ar g cgt Ar g gtt Val gct Al a ggt Gy ggt Gy aag Lys 120 tct Ser tgg Tr p ggt Gy aca Thr ggt Gy cca Pr o ttc Phe t ac Tyr ct g Leu tgc Cys at t SIle gaa G u acc Thr gga Gy acc Thr aca Thr at c SIle tgg Tr p at t SIle 75 gcg Al a at c SIle cca Pr o gtt Val tct Ser 155 gca Al a caa G n gag G u gct Al a ct a Leu ttc Phe t ac Tyr ct g Leu gag G u gac Asp 140 act Thr gt a Val ctc aac Leu Asn 15 gac gac Asp Asp gac cca Asp Pro ctc ggc Leu G y cct tgg Pro Trp ttg tac Leu Tyr 95 ctg gga Leu G y 110 atc gca I e Al a cgc acc Arg Thr ggt cgt G y Arg gcc ggc Al a G y gcg Al a gtt Val gct Al a at c SIle gag G u 80 acc Thr ttt Phe gt a Val at c SIle cgc Ar g 160 at g et 1 at g Net acc Thr gag G u at t SIle t ac Tyr cca Pr o gca Al a cag G n gtt Val 145 aag Lys agc Ser at t SIle aag Lys ggt Gy aag Lys at g et gtt Val cgt Ar g 130 gca Al a ct g Leu aat Asn gca Al a cgc Ar g gga Gy gca Al a ct g Leu gt c Val 115 cgc Ar g ctt Leu at c SIle gag G u t ac Tyr gct Al a ctt Leu cac Hi s ggt Gy 100 ctt Leu cac Hi s ct c Leu at g bet agt aac aac aac Ser Asn Asn Asn gat Asp cgc Ar g gca Al a ggg Gy gga Gy att SIe t at Tyr gac Asp aat Asn gga Gy 165 ctg acc atc at c Leu Thr Ile Ile gct cca Ala Pro Page 849 Gene Sequence 24-2-06.txt 170 175 180 atg ggc ggt atg atc aag aac cct tgg aat cct aag gaa ggc cca atg 691 IVet y y y et lie Lys Asn Pro Trp Asn Pro Lys G u y Pro IVet 185 190 195 gac gtt cag ggt gac ggc acc ctg tgg act tcc ggt tgg act ctc gtt 739 Asp Val G n y Asp G y Thr Leu Trp Thr Ser y Trp Thr Leu Val 200 205 210 gag aac gac gtc aag gtt tac ctc ggc cgc gac act gca gca att gcg 787 G u Asn Asp Val Lys Val Tyr Leu y Arg Asp Thr Ala Ala Ile Ala 215 220 225 gag tcc cac acc gat gca acc ggt gag cac tgg tca acc act ggt gtt 835 G u Ser His Thr Asp Al a Thr G y G u His Trp Ser Thr Thr G y Val 230 235 240 245 tcc cgc ctg gtt cgt atg cgc cca gaa gat ctg gca gca gca tcc atg 883 Ser Arg Leu Val Arg IVet Arg Pro G u Asp Leu Ala Ala Ala Ser IVet 250 255 260 gaa act gtc ttc cca ctt cca gct gaa atg gtg aac gac ggt gct gaa 931 G u Thr Val Phe Pro Leu Pro Ala G u Iet Val Asn Asp Gy Ala G u 265 270 275 tac gat cct gcg aag gac gtc tac gag cac caa atg cac tcg gtg cac 979 Tyr Asp Pro Ala Lys Asp Val Tyr G u His G n IVet His Ser Val His 280 285 290 ggc cca cgc aac gca gtt atg ttg atc cgt ctc cgt acc gct gac gct 1027 Sy Pro Arg Asn Ala Val IVet Leu lie Arg Leu Arg Thr Ala Asp Ala 295 300 305 gaa aag gtt atc gaa cgc gaa ggc cag gag tcc ttc cac tac ggt gac 1075 G u Lys Val Ile u Arg G u G y Gn Gu Ser Phe His Tyr G y Asp 310 315 320 325 tac tac gct tac tcc aag att tgt aca cac att ggt tgc cca acc tca 1123 Tyr Tyr Ala Tyr Ser Lys IIe Cys Thr His lie G y Cys Pro Thr Ser 330 335 340 ctg tac gag gct cag acc aat cgt att ctg tgc cca tgt cac cag tcg 1171 Leu Tyr G u Ala G n Thr Asn Arg lie Leu Cys Pro Cys His G n Ser 345 350 355 cag ttt gac gca ttg cac tac gga aag cca gtc ttt gga cct gct gcc 1219 G n Phe Asp Ala Leu His Tyr G y Lys Pro Val Phe G y Pro Ala Ala 360 365 370 cgt gca ctg cca cag ctg cca att acc gtt gat gaa gag ggc tac ctc 1267 Arg Ala Leu Pro G n Leu Pro lie Thr Val Asp G u G u y Tyr Leu 375 380 385 atc gcc gct ggt aac ttc att gag cca ctc ggc cct gca ttc tgg gag 1315 lie Ala Ala G y Asn Phe lie G u Pro Leu G y Pro Al a Phe Trp G u 390 395 400 405 cgt aag tca tgagtctagc taccgtggga aac 1347 Arg Lys Ser <210> 666 <211> 408 <212> PRT <213> Cor ynebact eri um gl ut am cum Page 850 Gene Sequence 24-2-06.txt <400> 666 lIet Ser Asn Asn Asn Asp Lys G n Tyr Thr Thr G n G u Leu Asn Ala 10 1 vet Thr G u SIle Tyr Pr o Al a G n Val 145 Lys Thr Lys Gy Thr 225 Ser Al a Asn vet Ar g 305 Phe Gy Asn Al a Ar g Gy Al a Leu Val 115 Ar g Leu SIle SIle Gy 195 Thr Al a Thr Al a Gy 275 Ser Al a Tyr Pr o 5 Asp Ar g Al a Gy Gy I le Tyr Asp Asn Gy 165 Pr o let Val Al a Val 245 et G u Hi s Al a Asp 325 Ser Al a G u Al a Leu G u Ser Lys Pr o 135 Ser Al a Gy Val Asn 215 Ser Ar g Thr Asp Pr o 295 Lys Tyr Tyr Ar g Ar g 40 Val Al a Gy Gy Lys 120 Ser Tr p Gy Gy G n 200 Asp Hi s Leu Val Pr o 280 Ar g Val Al a G u Leu Phe Thr Thr Leu Leu 105 Phe G u G n Gy et 185 Gy Val Thr Val Phe 265 Al a Asn SIle Tyr Al a 345 Thr SIle Tr p SIle Al a SIle Pr o Val Ser 155 Al a Lys Gy Val Al a 235 let Leu Asp Val Ar g 315 Lys Thr Leu Asn Val Tr p Thr Ser G u 125 Ar g Leu Leu Pr o Leu 205 Leu Gy Pr o Al a Tyr 285 Leu Gy Cys Ar g Asp Pr o Gy Tr p Tyr Gy Al a Thr Ar g Gy 175 Asn Thr Ar g Hi s Asp 255 let Hi s Ar g G u Hi s 335 Leu Pro Cys His G n Ser G n Phe Asp Ala Leu His Tyr G y Lys Pro Val Page 851 ene Sequence 24- 2- 06. txt 355 360 365 Phe G31y Pro Ala Ala Arg Ala Leu Pro On Leu Pro lieThr Val Asp 370 375 380 G31u 31u G31y Tyr Leu IlIe Al aAl a (1y As n Phe Ilie Gu Pro Leu G1y 385 390 395 400 Pr o Al a Phe Tr p G31u Ar g Lys Ser 405 <210O> 667 <21 1> 1053 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1030) <223> RXA02740 <400> 667 aacgcaaggt gcacact cac cgcagggat a t t taaat aat t at gagt gat t tgaaaat gc aacgt tct gg aggagaaccc aaggact cac aact taaat a gcc Al a gcc Al a gt g ValI gcc Al a gga G31y g ac As p ct g Le u ac g Thr ac g Thr ct c Le u 1 50 at t I Ie at c I Ie at c lIe ac c Thr act Thr t cc Ser ct g Le u tt c Ph e 120 at g vb t ggc (31y ct a Le u ac a Thr ct g Le u aac As n gct Al a tt t Ph e t gc cys t ac Tyr at c I Ie gca Al a aag Ly s ct t Le u gt g ValI gt g ValI cct Pr o t gg Tr p t cg Ser tt t Ph e t gg Tr p 140 at c I Ie ccc Pr o c ag O31n tt c Ph e 45 gca Al a tt g Le u gt c ValI at g vb t gt c ValI 125 ggc G31y gt t ValI ag g Ar g gct Al a ggt G31y g ac As p gt g ValI ct g Le u ct c Le u 110 t ac Tyr gga G31y g at As p gt t ValI 15 g aa O31u t cc Ser cgc Ar g ac a Thr 95 gcc Al a ac c Thr gcc Al a c ag (31n at t I Ie cgc Ar g t gg Tr p g at As p c ac Hi s 80 gt g ValI ggc aag Ly s gca Al a tt t Ph e 1 tt g Le u 1 g aa O31u ggt G31y at g vb t at t I Ie ac c Thr gcc Al a at c I Ie t gg Tr p ggt G31y 145 gag (31u ct c Le u gag O31u ggt G31y g at As p gt g ValI ag c Ser tt c Ph e ct g Le u 130 t gt cys cca Pr o gac acg at Asp Thr 11 ct Le 2 aa As gc Al cg Ar aa As t t Le 10 t t Le cg Ar cc Pr gt Va c aag e Lys t gt c u Val 0 c at t n I Ie c gcc a Al a c at g g I'b t t cgc n Ar g g t gg u Tr p 0 g at c u I Ie c cgc g Ar g a gt g o Val t cca I Pr o Page 852