AU783707B2 - Corynebacterium glutamicum genes encoding proteins involved in membrane synthesis and membrane transport - Google Patents

Description

WO 01/00805 PCT/IBOO/00926 -1- CORYNEBACTERIUM GLUTAMICUM GENES ENCODING PROTEINS INVOLVED IN MEMBRANE SYNTHESIS AND MEMBRANE TRANSPORT Related Applications This application claims priority to prior filed U.S. Provisional Patent Application Serial No. 60/141031, filed June 25, 1999. This application also claims priority to German Patent Application No. 19931454.3, filed July 8, 1999, German Patent Application No. 19931478.0, filed July 8, 1999, German Patent Application No.

19931563.9, filed July 8, 1999, German Patent Application No. 19932122.1, filed July 9, 1999, German Patent Application No. 19932124.8, filed 99709, German Patent Application No. 19932125.6, filed July 9, 1999, German Patent Application No.

19932128.0, filed July 9, 1999, German Patent Application No. 19932180.9, filed July 9, 1999, German Patent Application No. 19932182.5, filed July 9, 1999, German Patent Application No. 19932190.6, filed July 9, 1999, German Patent Application No.

19932191.4, filed July 9, 1999, German Patent Application No. 19932209.0, filed July 9, 1999, German Patent Application No. 19932212.0, filed July 9, 1999, German Patent Application No. 19932227.9, filed July 9, 1999, German Patent Application No.

19932228.7, filed July 9, 1999, German Patent Application No. 19932229.5, filed 99070, German Patent Application No. 19932230.9, filed July 9, 1999, German Patent Application No. 19932927.3, filed July 14, 1999, German Patent Application No.

19933005.0, filed July 14, 1999, German Patent Application No. 19933006.9, filed July 14, 1999, German Patent Application No. 19940764.9, filed August 27, 1999, German Patent Application No. 19940765.7, filed August 27, 1999, German Patent Application No. 19940766.5, filed August 27, 1999, German Patent Application No. 19940830.0, filed August 27, 1999, German Patent Application No. 19940831.9, filed August 27, 1999, German Patent Application No. 19940832.7, filed August 27, 1999, German Patent Application No. 19940833.5, filed August 27, 1999, German Patent Application No. 19941378.9 filed August 31, 1999, German Patent Application No. 19941379.7, filed August 31, 1999, German Patent Application No. 19941395.9, filed August 31, 1999, German Patent Application No. 19942077.7, filed September 3, 1999, German Patent Application No. 19942078.5, filed September 3, 1999, German Patent WO 01/00805 PCT/IB00/00926 -2- Application No. 19942079.3, filed September 3, 1999, and German Patent Application No. 19942088.2, filed September 3, 1999. The entire contents of all of the above referenced applications are hereby expressly incorporated herein by this reference.

Background of the Invention Certain products and by-products of naturally-occurring metabolic processes in cells have utility in a wide array of industries, including the food, feed, cosmetics, and pharmaceutical industries. These molecules, collectively termed 'fine chemicals', include organic acids, both proteinogenic and non-proteinogenic amino acids, nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, and enzymes. Their production is most conveniently performed through the large-scale culture of bacteria developed to produce and secrete large quantities of one or more desired molecules. One particularly useful organism for this purpose is Corynebacterium glutamicum, a gram positive, nonpathogenic bacterium. Through strain selection, a number of mutant strains have been developed which produce an array of desirable compounds. However, selection of strains improved for the production of a particular molecule is a time-consuming and difficult process.

Summary of the Invention The invention provides novel bacterial nucleic acid molecules which have a variety of uses. These uses include the identification of microorganisms which can be used to produce fine chemicals, the modulation of fine chemical production in C.

glutamicum or related bacteria, the typing or identification of C glutamicum or related bacteria, as reference points for mapping the C. glutamicum genome, and as markers for transformation. These novel nucleic acid molecules encode proteins, referred to herein as membrane construction and membrane transport (MCT) proteins.

C. glutamicum is a gram positive, aerobic bacterium which is commonly used in industry for the large-scale production of a variety of fine chemicals, and also for the degradation of hydrocarbons (such as in petroleum spills) and for the oxidation of terpenoids. The MCT nucleic acid molecules of the invention, therefore, can be used to identify microorganisms which can be used to produce fine chemicals, by WO 01/00805 PCT/IB00/00926 -3fermentation processes. Modulation of the expression of the MCT nucleic acids of the invention, or modification of the sequence of the MCT nucleic acid molecules of the invention, can be used to modulate the production of one or more fine chemicals from a microorganism to improve the yield or production of one or more fine chemicals from a Corynebacterium or Brevibacterium species).

The MCT nucleic acids of the invention may also be used to identify an organism as being Corynebacterium glutamicum or a close relative thereof, or to identify the presence of C. glutamicum or a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of C.

glutamicum genes; by probing the extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a C. glutamicum gene which is unique to this organism, one can ascertain whether this organism is present. Although Corynebacterium glutamicum itself is nonpathogenic, it is related to species pathogenic in humans, such as Corynebacterium diphtheriae (the causative agent of diphtheria); the detection of such organisms is of significant clinical relevance.

The MCT nucleic acid molecules of the invention may also serve as reference points for mapping of the C. glutamicum genome, or of genomes of related organisms.

Similarly, these molecules, or variants or portions thereof, may serve as markers for genetically engineered Corynebacterium or Brevibacterium species.

The MCT proteins encoded by the novel nucleic acid molecules of the invention are capable of, for example, performing a function involved in the metabolism the biosynthesis or degradation) of compounds necessary for membrane biosynthesis, or of assisting in the transmembrane transport of one or more compounds either into or out of the cell. Given the availability of cloning vectors for use in Corynebacterium glutamicum, such as those disclosed in Sinskey et al., U.S. Patent No. 4,649,119, and techniques for genetic manipulation of C. glutamicum and the related Brevibacterium species lactofermentum) (Yoshihama et al, J. Bacteriol. 162: 591-597 (1985); Katsumata et al., J. Bacteriol. 159: 306-311 (1984); and Santamaria et al., J. Gen.

Microbiol. 130: 2237-2246 (1984)), the nucleic acid molecules of the invention may be utilized in the genetic engineering of this organism to make it a better or more efficient producer of one or more fine chemicals. This improved production or efficiency of WO 01/00805 PCTIB00/00926 -4production of a fine chemical may be due to a direct effect of manipulation of a gene of the invention, or it may be due to an indirect effect of such manipulation.

There are a number of mechanisms by which the alteration of an MCT protein of the invention may directly affect the yield, production, and/or efficiency of production of a fine chemical from a C. glutamicum strain incorporating such an altered protein.

Those MCT proteins involved in the export of fine chemical molecules from the cell may be increased in number or activity such that greater quantities of these compounds are secreted to the extracellular medium, from which they are more readily recovered.

Similarly, those MCT proteins involved in the import of nutrients necessary for the biosynthesis of one or more fine chemicals phosphate, sulfate, nitrogen compounds, etc.) may be increased in number or activity such that these precursors, cofactors, or intermediate compounds are increased in concentration within the cell.

Further, fatty acids and lipids themselves are desirable fine chemicals; by optimizing the activity or increasing the number of one or more MCT proteins of the invention which participate in the biosynthesis of these compounds, or by impairing the activity of one or more MCT proteins which are involved in the degradation of these compounds, it may be possible to increase the yield, production, and/or efficiency of production of fatty acid and lipid molecules from C. glutamicum.

The mutagenesis of one or more MCT genes of the invention may also result in MCT proteins having altered activities which indirectly impact the production of one or more desired fine chemicals from C .glutamicum. For example, MCT proteins of the invention involved in the export of waste products may be increased in number or activity such that the normal metabolic wastes of the cell (possibly increased in quantity due to the overproduction of the desired fine chemical) are efficiently exported before they are able to damage nucleotides and proteins within the cell (which would decrease the viability of the cell) or to interfere with fine chemical biosynthetic pathways (which would decrease the yield, production, or efficiency of production of the desired fine chemical). Further, the relatively large intracellular quantities of the desired fine chemical may in itself be toxic to the cell, so by increasing the activity or number of transporters able to export this compound from the cell, one may increase the viability of the cell in culture, in turn leading to a greater number of cells in the culture producing the desired fine chemical. The MCT proteins of the invention may also be manipulated 06/10 '05 THU 10:54 FAX 61 2 9888 7600 WATERMARK 1&1007 such that the relative amounts of different lipid and fatty acid molecules are produced. This may have a profound effect on the lipid composition of the membrane of the cell. Since each type of lipid has different physical properties, an alteration in the lipid composition of a membrane may significantly alter membrane fluidity. Changes In membrane fluidity can impact the transport of molecules across the membrane, as well as the integrity of the cell, both of which have a profound effect on the production of fine chemicals from C. glutamicum in large-scale fermentative culture.

The invention provides novel nucleic acid molecules which encode proteins, referred to herein as MCT proteins, which are capable of, for example, participating in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes. Nucleic acid molecules encoding an MCT protein are referred S 15 to herein as MCT nucleic acid molecules. In a preferred embodiment, the MCT protein participates in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes. Examples of such proteins include those encoded by the genes set forth in Table 1.

20 The following embodiments, the subject of the invention of this application are specifically disclosed herein: An isolated Corynebacterium glutamicum nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:5, or a complement thereof.

25 An isolated nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:6, or a complement thereof.

An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:6, or a complement thereof.

COMS ID No: SBMI-01674575 Received by IP Australia: Time 11:03 Date 2005-10-06 11/10 '05 TUE 14:56 FAX 61 2 9888 7600 WATERMARK 11004 SAn isolated nucleic acid molecule comprising a nucleotide sequence which is functionally equivalent, and at least 50% identical, to the entire nucleotide sequence set forth in SEQ ID NO:5, or a complement thereof.

An isolated nucleic acid molecule comprising a fragment of at least contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO:5, or a complement thereof.

An isolated nucleic acid molecule comprising the nucleic acid molecule of any one of claims and a nucleotide sequence encoding a heterologous polypeptide.

An isolated polypeptide comprising an amino acid sequence set forth in SEQ ID NO:6.

An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence set 15 forth in SEQ ID NO:6.

An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is functionally equivalent, and at least 50% identical, to the entire nucleic acid sequence set forth in SEQ ID 20 An isolated polypeptide comprising an amino acid sequence which is functionally equivalent, and at least 50% identical, to the entire amino acid sequence set forth in SEQ ID NO:6.

An isolated polypeptide comprising a fragment of the amino acid sequence set .forth in SEQ ID NO:6, wherein said fragment 25 maintains an ammonium transport system activity.

A host cell comprising a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:5, wherein the nucleic acid molecule is disrupted.

A host cell comprising a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:5, wherein the nucleic acid molecule comprises one or more nucleic acid modifications as compared to the sequence set forth.

COMS ID No: SBMI-01710428 Received by IP Australia: Time 15:03 Date 2005-10-11 11/10 '05 TUE 14:56 FAX 61 2 9888 7600 WATERMARK to 005 A host cell comprising a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:5, wherein the regulatory region of the nucleic acid molecule is modified relative to the wild-type regulatory region of the molecule.

Accordingly, one aspect of the invention pertains to isolated nucleic acid molecules cDNAs, DNAs, or RNAs) comprising a nucleotide sequence encoding an MCT protein or biologically active portions thereof, as well as nucleic acid fragments suitable as primers or hybridization probes for the detection or amplification of MCT encoding nucleic acid DNA or mRNA). In particularly preferred embodiments, the isolated nucleic acid molecule comprises one of the nucleotide sequences set forth as the odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO:I, SEQ ID NO:3, SEQ ID NO:5, SEQ ID forth as the odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID or the coding 15 region or a complement thereof of one of these nucleotide sequences. In other S. particularly preferred embodiments, the Isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes to or is at least about 50%, preferably at least about 60%, more preferably at least about or 90%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% 20 or more homologous to a nucleotide sequence set forth as an odd-numbered SEQ ID NO in the Sequence Listing SEQ ID NO:1, SEQ ID NO:3, SEQ ID SEQ ID or a portion g** S *.o COMS ID No: SBMI-01710428 Received by IP Australia: Time 15:03 Date 2005-10-11 WO 01/00805 PCT/IB00/00926 -6thereof. In other preferred embodiments, the isolated nucleic acid molecule encodes one of the amino acid sequences set forth as an even-numbered SEQ ID NO in the Sequence Listing SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID The preferred MCT proteins of the present invention also preferably possess at least one of the MCT activities described herein.

In another embodiment, the isolated nucleic acid molecule encodes a protein or portion thereof wherein the protein or portion thereof includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence having an even-numbered SEQ ID NO: in the Sequence Listing), e.g., sufficiently homologous to an amino acid sequence of the invention such that the protein or portion thereof maintains an MCT activity. Preferably; the protein or portion thereof encoded by the nucleic acid molecule maintains the ability to participate in the metabolism of compounds necessary for the construction of cellular membranes in C.

glutamicum, or in the transport of molecules across these membranes. In one embodiment, the protein encoded by the nucleic acid molecule is at least about preferably at least.about 60%, and more preferably at least about 70%, 80%, or 90% and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an amino acid sequence of the invention an entire amino acid sequence selected from those having an even-numbered SEQ ID NO in the Sequence Listing). In another preferred embodiment, the protein is a full length C. glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention (encoded by an open reading frame shown in the corresponding odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO:1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID In another preferred embodiment, the isolated nucleic acid molecule is derived from C. glutamicum and encodes a protein an MCT fusion protein) which includes a biologically active domain which is at least about 50% or more homologous to one of the amino acid sequences of the invention a sequence of one of the even-numbered SEQ ID NOs in the Sequence Listing) and is able to participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes, or has one or more of the activities set forth in Table 1, and which also includes heterologous nucleic acid sequences encoding a heterologous polypeptide or regulatory regions.

WO 01/00805 PCT/IBOO/00926 -7- In another embodiment, the isolated nucleic acid molecule is at least nucleotides in length and hybridizes under stringent conditions to a nucleic acid molecule comprising a nucleotide sequence of the invention a sequence of an oddnumbered SEQ ID NO in the Sequence Listing). Preferably, the isolated nucleic acid molecule corresponds to a naturally-occurring nucleic acid molecule. More preferably, the isolated nucleic acid encodes a naturally-occurring C. glutamicum MCT protein, or a biologically active portion thereof.

Another aspect of the invention pertains to vectors, recombinant expression vectors, containing the nucleic acid molecules of the invention, and host cells into which such vectors have been introduced. In one embodiment, such a host cell is used to produce an MCT protein by culturing the host cell in a suitable medium. The MCT protein can be then isolated from the medium or the host cell.

Yet another aspect of the invention pertains to a genetically altered microorganism in which an MCT gene has been introduced or altered. In one embodiment, the genome of the microorganism has been altered by introduction of a nucleic acid molecule of the invention encoding wild-type or mutated MCT sequence as a transgene. In another embodiment, an endogenous MCT gene within the genome of the microorganism has been altered, functionally disrupted, by homologous recombination with an altered MCT gene. In another embodiment, an endogenous or introduced MCT gene in a microorganism has been altered by one or more point mutations, deletions, or inversions, but still encodes a functional MCT protein. In still another embodiment, one or more of the regulatory regions a promoter, repressor, or inducer) of an MCT gene in a microorganism has been altered by deletion, truncation, inversion, or point mutation) such that the expression of the MCT gene is modulated. In a preferred embodiment, the microorganism belongs to the genus Corynebacterium or Brevibacterium, with Corynebacterium glutamicum being particularly preferred. In a preferred embodiment, the microorganism is also utilized for the production of a desired compound, such as an amino acid, with lysine being particularly preferred.

In another aspect, the invention provides a method of identifying the presence or activity of Cornyebacterium diphtheriae in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention the WO 01/00805 PCT/IB00/00926 -8sequences set forth in the Sequence Listing as SEQ ID NOs 1 through 676) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject.

Still another aspect of the invention pertains to an isolated MCT protein or a portion, a biologically active portion, thereof. In a preferred embodiment, the isolated MCT protein or portion thereof can participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes. In another preferred embodiment, the isolated MCT protein or portion thereof is sufficiently homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: in the Sequence Listing) such that the protein or portion thereof maintains the ability to participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes.

The invention also provides an isolated preparation of an MCT protein. In preferred embodiments, the MCT protein comprises an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing).

In another preferred embodiment, the invention pertains to an isolated full length protein which is substantially homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) (encoded by an open reading frame set forth in a corresponding odd-numbered SEQ ID NO: of the Sequence Listing In yet another embodiment, the protein is at least about preferably at least about 60%, and more preferably at least about 70%, 80%, or and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing). In other embodiments, the isolated MCT protein comprises an amino acid sequence which is at least about 50% or more homologous to one of the amino acid sequences of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) and is able to participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes, or has one or more of the activities set forth in Table 1.

Alternatively, the isolated MCT protein can comprise an amino acid sequence which is encoded by a nucleotide sequence which hybridizes, hybridizes under WO 01/00805 PCT/IBOO/00926 -9stringent conditions, or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80%, or 90%, and even more preferably at least about 96%, 97%, or 99% or more homologous, to a nucleotide sequence of one of the even-numbered SEQ ID NOs set forth in the Sequence Listing. It is also preferred that the preferred forms of MCT proteins also have one or more of the MCT bioactivities described herein.

The MCT polypeptide, or a biologically active portion thereof, can be operatively linked to a non-MCT polypeptide to form a fusion protein. In preferred embodiments, this fusion protein has an activity which differs from that of the MCT protein alone. In other preferred embodiments, this fusion protein participate in the metabolism of compounds necessary for the construction of cellular membranes in C.

glutamicum, or in the transport of molecules across these membranes. In particularly preferred embodiments, integration of this fusion protein into a host cell modulates production of a desired compound from the cell.

In another aspect, the invention provides methods for screening molecules which modulate the activity of an MCT protein, either by interacting with the protein itself or a substrate or binding partner of the MCT protein, or by modulating the transcription or translation of an MCT nucleic acid molecule of the invention.

Another aspect of the invention pertains to a method for producing a fine chemical. This method involves the culturing of a cell containing a vector directing the expression of an MCT nucleic acid molecule of the invention, such that a fine chemical is produced. In a preferred embodiment, this method further includes the step of obtaining a cell containing such a vector, in which a cell is transfected with a vector directing the expression of an MCT nucleic acid. In another preferred embodiment, this method further includes the step of recovering the fine chemical from the culture. In a particularly preferred embodiment, the cell is from the genus Corynebacterium or Brevibacterium, or is selected from those strains set forth in Table 3.

Another aspect of the invention pertains to methods for modulating production of a molecule from a microorganism. Such methods include contacting the cell with an agent which modulates MCT protein activity or MCT nucleic acid expression such that a cell associated activity is altered relative to this same activity in the absence of the agent. In a preferred embodiment, the cell is modulated for one or more C. glutamicum WO 01/00805 PCT/IB00/00926 metabolic pathways for cell membrane components or is modulated for the transport of compounds across such membranes, such that the yields or rate of production of a desired fine chemical by this microorganism is improved. The agent which modulates MCT protein activity can be an agent which stimulates MCT protein activity or MCT nucleic acid expression. Examples of agents which stimulate MCT protein activity or MCT nucleic acid expression include small molecules, active MCT proteins, and nucleic acids encoding MCT proteins that have been introduced into the cell. Examples of agents which inhibit MCT activity or expression include small molecules and antisense MCT nucleic acid molecules.

Another aspect of the invention pertains to methods for modulating yields of a desired compound from a cell, involving the introduction of a wild-type or mutant MCT gene into a cell, either maintained on a separate plasmid or integrated into the genome of the host cell. If integrated into the genome, such integration can be random, or it can take place by homologous recombination such that the native gene is replaced by the introduced copy, causing the production of the desired compound from the cell to be modulated. In a preferred embodiment, said yields are increased. In another preferred embodiment, said chemical is a fine chemical. In a particularly preferred embodiment, said fine chemical is an amino acid. In especially preferred embodiments, said amino acid is L-lysine.

Detailed Description of the Invention The present invention provides MCT nucleic acid and protein molecules which are involved in the metabolism of cellular membrane components in C. glutamicumn or in the transport of compounds across such membranes. The molecules of the invention may be utilized in the modulation of production of fine chemicals from microorganisms, such as C. glutamicum, either directly where overexpression or optimization of a fatty acid biosynthesis protein has a direct impact on the yield, production, and/or efficiency of production of the fatty acid from modified C. glufamicum), or may have an indirect impact which nonetheless results in an increase of yield, production, and/or efficiency of production of the desired compound where modulation of the metabolism of cell membrane components results in alterations in the yield, production, WO 01/00805 PCT/IB00/00926 -11 and/or efficiency of production or the composition of the cell membrane, which in turn may impact the production of one or more fine chemicals). Aspects of the invention are further explicated below.

I. Fine Chemicals The term 'fine chemical' is art-recognized and includes molecules produced by an organism which have applications in various industries, such as, but not limited to, the pharmaceutical, agriculture, and cosmetics industries. Such compounds include organic acids, such as tartaric acid, itaconic acid, and diaminopimelic acid, both proteinogenic and non-proteinogenic amino acids, purine and pyrimidine bases, nucleosides, and nucleotides (as described e.g. in Kuninaka, A. (1996) Nucleotides and related compounds, p. 561-612, in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, and references contained therein), lipids, both saturated and unsaturated fatty acids arachidonic acid), diols propane diol, and butane diol), carbohydrates hyaluronic acid and trehalose), aromatic compounds aromatic amines, vanillin, and indigo), vitamins and cofactors (as described in Ullmann's Encyclopedia of Industrial Chemistry, vol. A27, "Vitamins", p. 443-613 (1996) VCH: Weinheim and references therein; and Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press, (1995)), enzymes, polyketides (Cane et al. (1998) Science 282: 63-68), and all other chemicals described in Gutcho (1983) Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086 and references therein. The metabolism and uses of certain of these fine chemicals are further explicated below.

A. Amino Acid Metabolism and Uses Amino acids comprise the basic structural units of all proteins, and as such are essential for normal cellular functioning in all organisms. The term "amino acid" is artrecognized. The proteinogenic amino acids, of which there are 20 species, serve as structural units for proteins, in which they are linked by peptide bonds, while the nonproteinogenic amino acids (hundreds of which are known) are not normally found in WO 01/00805 PCT/IB00/00926 -12proteins (see Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97 VCH: Weinheim (1985)). Amino acids may be in the D- or L- optical configuration, though Lamino acids are generally the only type found in naturally-occurring proteins.

Biosynthetic and degradative pathways of each of the 20 proteinogenic amino acids have been well characterized in both prokaryotic and eukaryotic cells (see, for example, Stryer, L. Biochemistry, 3 r d edition, pages 578-590 (1988)). The 'essential' amino acids (histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine), so named because they are generally a nutritional requirement due to the complexity of their biosyntheses, are readily converted by simple biosynthetic pathways to the remaining 11 'nonessential' amino acids (alanine, arginine, asparagine, aspartate, cysteine, glutamate, glutamine, glycine, proline, serine, and tyrosine). Higher animals do retain the ability to synthesize some of these amino acids, but the essential amino acids must be supplied from the diet in order for normal protein synthesis to occur.

Aside from their function in protein biosynthesis, these amino acids are interesting chemicals in their own right, and many have been found to have various applications in the food, feed, chemical, cosmetics, agriculture, and pharmaceutical industries. Lysine is an important amino acid in the nutrition not only of humans, but also ofmonogastric animals such as poultry and swine. Glutamate is most commonly used as a flavor additive (mono-sodium glutamate, MSG) and is widely used throughout the food industry, as are aspartate, phenylalanine, glycine, and cysteine. Glycine, Lmethionine and tryptophan are all utilized in the pharmaceutical industry. Glutamine, valine, leucine, isoleucine, histidine, arginine, proline, serine and alanine are of use in both the pharmaceutical and cosmetics industries. Threonine, tryptophan, and D/ Lmethionine are common feed additives. (Leuchtenberger, W. (1996) Amino aids technical production and use, p. 466-502 in Rehm et al. (eds.) Biotechnology vol. 6, chapter 14a, VCH: Weinheim). Additionally, these amino acids have been found to be useful as precursors for the synthesis of synthetic amino acids and proteins, such as Nacetylcysteine, S-carboxymethyl-L-cysteine, (S)-5-hydroxytryptophan, and others described in Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97, VCH: Weinheim, 1985.

The biosynthesis of these natural amino acids in organisms capable of producing them, such as bacteria, has been well characterized (for review of bacterial WO 01/00805 PCT/IB00/00926 13amino acid biosynthesis and regulation thereof, see Umbarger, H.E.(1978) Ann. Rev.

Biochem. 47: 533-606). Glutamate is synthesized by the reductive amination of aketoglutarate, an intermediate in the citric acid cycle. Glutamine, proline, and arginine are each subsequently produced from glutamate. The biosynthesis of serine is a threestep process beginning with 3-phosphoglycerate (an intermediate in glycolysis), and resulting in this amino acid after oxidation, transamination, and hydrolysis steps. Both cysteine and glycine are produced from serine; the former by the condensation of homocysteine with serine, and the latter by the transferal of the side-chain P-carbon atom to tetrahydrofolate, in a reaction catalyzed by serine transhydroxymethylase.

Phenylalanine, and tyrosine are synthesized from the glycolytic and pentose phosphate pathway precursors erythrose 4-phosphate and phosphoenolpyruvate in a 9-step biosynthetic pathway that differ only at the final two steps after synthesis ofprephenate.

Tryptophan is also produced from these two initial molecules, but its synthesis is an 11step pathway. Tyrosine may also be synthesized from phenylalanine, in a reaction catalyzed by phenylalanine hydroxylase. Alanine, valine, and leucine are all biosynthetic products of pyruvate, the final product of glycolysis. Aspartate is formed from oxaloacetate, an intermediate of the citric acid cycle. Asparagine, methionine, threonine, and lysine are each produced by the conversion of aspartate. Isoleucine is formed from threonine. A complex 9-step pathway results in the production of histidine from 5-phosphoribosyl-1 -pyrophosphate, an activated sugar.

Amino acids in excess of the protein synthesis needs of the cell cannot be stored, and are instead degraded to provide intermediates for the major metabolic pathways of the cell (for review see Stryer, L. Biochemistry 3 rd ed. Ch. 21 "Amino Acid Degradation and the Urea Cycle" p. 495-516 (1988)). Although the cell is able to convert unwanted amino acids into useful metabolic intermediates, amino acid production is costly in terms of energy, precursor molecules, and the enzymes necessary to synthesize them.

Thus it is not surprising that amino acid biosynthesis is regulated by feedback inhibition, in which the presence of a particular amino acid serves to slow or entirely stop its own production (for overview of feedback mechanisms in amino acid biosynthetic pathways, see Stryer, L. Biochemistry, 3 rd ed. Ch. 24: "Biosynthesis of Amino Acids and Heme" p.

575-600 (1988)). Thus, the output of any particular amino acid is limited by the amount of that amino acid present in the cell.

WO 01/00805 PCT/IB00/00926 -14- B. Vitamin, Cofactor, and Nutraceutical Metabolism and Uses Vitamins, cofactors, and nutraceuticals comprise another group of molecules which the higher animals have lost the ability to synthesize and so must ingest, although they are readily synthesized by other organisms such as bacteria. These molecules are either bioactive substances themselves, or are precursors of biologically active substances which may serve as electron carriers or intermediates in a variety of metabolic pathways. Aside from their nutritive value, these compounds also have significant industrial value as coloring agents, antioxidants, and catalysts or other processing aids. (For an overview of the structure, activity, and industrial applications of these compounds, see, for example, Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996.) The term "vitamin" is artrecognized, and includes nutrients which are required by an organism for normal functioning, but which that organism cannot synthesize by itself. The group of vitamins may encompass cofactors and nutraceutical compounds. The language "cofactor" includes nonproteinaceous compounds required for a normal enzymatic activity to occur. Such compounds may be organic or inorganic; the cofactor molecules of the invention are preferably organic. The term "nutraceutical" includes dietary supplements having health benefits in plants and animals, particularly humans. Examples of such molecules are vitamins, antioxidants, and also certain lipids polyunsaturated fatty acids).

The biosynthesis of these molecules in organisms capable of producing them, such as bacteria, has been largely characterized (Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley Sons; Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept.

1-3, 1994 at Penang, Malaysia, AOCS Press: Champaign, IL X, 374 S).

Thiamin (vitamin Bi) is produced by the chemical coupling of pyrimidine and thiazole moieties. Riboflavin (vitamin B 2 is synthesized from (GTP) and ribose-5'-phosphate. Riboflavin, in turn, is utilized for the synthesis of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). The family of WO 01/00805 PCT/IB00/00926 compounds collectively termed 'vitamin B 6 pyridoxine, pyridoxamine, pyridoxaand the commercially used pyridoxin hydrochloride) are all derivatives of the common structural unit, 5-hydroxy-6-methylpyridine. Pantothenate (pantothenic acid, (R)-(+)-N-(2,4-dihydroxy-3,3-dimethyl- -oxobutyl)-p-alanine) can be produced either by chemical synthesis or by fermentation. The final steps in pantothenate biosynthesis consist of the ATP-driven condensation of p-alanine and pantoic acid. The enzymes responsible for the biosynthesis steps for the conversion to pantoic acid, to 3alanine and for the condensation to panthotenic acid are known. The metabolically active form of pantothenate is Coenzyme A, for which the biosynthesis proceeds in enzymatic steps. Pantothenate, pyridoxal-5'-phosphate, cysteine and ATP are the precursors of Coenzyme A. These enzymes not only catalyze the formation of panthothante, but also the production of (R)-pantoic acid, (R)-pantolacton, panthenol (provitamin Bs), pantetheine (and its derivatives) and coenzyme A.

Biotin biosynthesis from the precursor molecule pimeloyl-CoA in microorganisms has been studied in detail and several of the genes involved have been identified. Many of the corresponding proteins have been found to also be involved in Fe-cluster synthesis and are members of the nifS class of proteins. Lipoic acid is derived from octanoic acid, and serves as a coenzyme in energy metabolism, where it becomes part of the pyruvate dehydrogenase complex and the a-ketoglutarate dehydrogenase complex. The folates are a group of substances which are all derivatives of folic acid, which is turn is derived from L-glutamic acid, p-amino-benzoic acid and 6methylpterin. The biosynthesis of folic acid and its derivatives, starting from the metabolism intermediates guanosine-5'-triphosphate (GTP), L-glutamic acid and pamino-benzoic acid has been studied in detail in certain microorganisms.

Corrinoids (such as the cobalamines and particularly vitamin B 1 2 and porphyrines belong to a group of chemicals characterized by a tetrapyrole ring system.

The biosynthesis of vitamin B 12 is sufficiently complex that it has not yet been completely characterized, but many of the enzymes and substrates involved are now known. Nicotinic acid (nicotinate), and nicotinamide are pyridine derivatives which are also termed 'niacin'. Niacin is the precursor of the important, coenzymes NAD (nicotinamide adenine dinucleotide) and NADP (nicotinamide adenine dinucleotide phosphate) and their reduced forms.

WO 01/00805 PCT/IB00/00926 -16- The large-scale production of these compounds has largely relied on cell-free chemical syntheses, though some of these chemicals have also been produced by largescale culture of microorganisms, such as riboflavin, Vitamin B 6 pantothenate, and biotin. Only Vitamin B 1 2 is produced solely by fermentation, due to the complexity of its synthesis. In vitro methodologies require significant inputs of materials and time, often at great cost.

C. Purine, Pyrimidine, Nucleoside and Nucleotide Metabolism and Uses Purine and pyrimidine metabolism genes and their corresponding proteins are important targets for the therapy of tumor diseases and viral infections. The language "purine" or "pyrimidine" includes the nitrogenous bases which are constituents of nucleic acids, co-enzymes, and nucleotides. The term "nucleotide" includes the basic structural units of nucleic acid molecules, which are comprised of a nitrogenous base, a pentose sugar (in the case of RNA, the sugar is ribose; in the case of DNA, the sugar is D-deoxyribose), and phosphoric acid. The language "nucleoside" includes molecules which serve as precursors to nucleotides, but which are lacking the phosphoric acid moiety that nucleotides possess. By inhibiting the biosynthesis of these molecules, or their mobilization to form nucleic acid molecules, it is possible to inhibit RNA and DNA synthesis; by inhibiting this activity in a fashion targeted to cancerous cells, the ability of tumor cells to divide and replicate may be inhibited. Additionally, there are nucleotides which do not form nucleic acid molecules, but rather serve as energy stores AMP) or as coenzymes FADand NAD).

Several publications have described the use of these chemicals for these medical indications, by influencing purine and/or pyrimidine metabolism Christopherson, R.I. and Lyons, S.D. (1990) "Potent inhibitors of de novo pyrimidine and purine biosynthesis as chemotherapeutic agents." Med Res. Reviews 10: 505-548). Studies of enzymes involved in purine and pyrimidine metabolism have been focused on the development of new drugs which can be used, for example, as immunosuppressants or anti-proliferants (Smith, (1995) "Enzymes in nucleotide synthesis." Curr. Opin.

Struct. Biol. 5: 752-757; (1995) Biochem Soc. Transact. 23: 877-902). However, purine and pyrimidine bases, nucleosides and nucleotides have other utilities: as intermediates in the biosynthesis of several fine chemicals thiamine, S-adenosyl-methionine, WO 01/00805 PCT/IB0O/00926 -17folates, or riboflavin), as energy carriers for the cell ATP or GTP), and for chemicals themselves, commonly used as flavor enhancers IMP or GMP) or for several medicinal applications (see, for example, Kuninaka, A. (1996) Nucleotides and Related Compounds in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, p. 561- 612). Also, enzymes involved in purine, pyrimidine, nucleoside, or nucleotide metabolism are increasingly serving as targets against which chemicals for crop protection, including fungicides, herbicides and insecticides, are developed.

The metabolism of these compounds in bacteria has been characterized (for reviews see, for example, Zalkin, H. and Dixon, J.E. (1992) "de novo purine nucleotide biosynthesis", in: Progress in Nucleic Acid Research and Molecular Biology, vol. 42, Academic Press:, p. 259-287; and Michal, G. (1999) "Nucleotides and Nucleosides", Chapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York). Purine metabolism has been the subject of intensive research, and is essential to the normal functioning of the cell. Impaired purine metabolism in higher animals can cause severe disease, such as gout. Purine nucleotides are synthesized from in a series of steps through the intermediate compound phosphate (IMP), resulting in the production of guanosine-5'-monophosphate (GMP) or (AMP), from which the triphosphate forms utilized as nucleotides are readily formed. These compounds are also utilized as energy stores, so their degradation provides energy for many different biochemical processes in the cell.

Pyrimidine biosynthesis proceeds by the formation of uridine-5'-monophosphate (UMP) from ribose-5-phosphate. UMP, in turn, is converted to cytidine-5'-triphosphate (CTP).

The deoxy- forms of all of these nucleotides are produced in a one step reduction reaction from the diphosphate ribose form of the nucleotide to the diphosphate deoxyribose form of the nucleotide. Upon phosphorylation, these molecules are able to participate in DNA synthesis.

D. Trehalose Metabolism and Uses Trehalose consists of two glucose molecules, bound in a, a-l,l linkage. It is commonly used in the food industry as a sweetener, an additive for dried or frozen foods, and in beverages. However, it also has applications in the pharmaceutical, cosmetics and biotechnology industries (see, for example, Nishimoto et al., (1998) U.S.

WO 01/00805 PCT/IB00/00926 -18- Patent No. 5,759,610; Singer, M.A. and Lindquist, S. (1998) Trends Biotech. 16: 460- 467; Paiva, C.L.A. and Panek, A.D. (1996) Biotech. Ann. Rev. 2: 293-314; and Shiosaka, M. (1997) J. Japan 172: 97-102). Trehalose is produced by enzymes from many microorganisms and is naturally released into the surrounding medium, from which it can be collected using methods known in the art.

II. Membrane Biosynthesis and Transmembrane Transport Cellular membranes serve a variety of functions in a cell. First and foremost, a membrane differentiates the contents of a cell from the surrounding environment, thus giving integrity to the cell. Membranes may also serve as barriers to the influx of hazardous or unwanted compounds, and also to the efflux of desired compounds.

Cellular membranes are by nature impervious to the unfacilitated diffusion of hydrophilic compounds such as proteins, water molecules and ions due to their structure: a bilayer of lipid molecules in which the polar head groups face outwards (towards the exterior and interior of the cell, respectively) and the nonpolar tails face inwards at the center of the bilayer, forming a hydrophobic core (for a general review of membrane structure and function, see Gennis, R.B. (1989) Biomembranes, Molecular Structure and Function, Springer: Heidelberg). This barrier enables cells to maintain a relatively higher concentration of desired compounds and a relatively lower concentration of undesired compounds than are contained within the surrounding medium, since the diffusion of these compounds is effectively blocked by the membrane.

However, the membrane also presents an effective barrier to the import of desired compounds and the export of waste molecules. To overcome this difficulty, cellular membranes incorporate many kinds of transporter proteins which are able to facilitate the transmembrane transport of different kinds of compounds. There are two general classes of these transport proteins: pores or channels and transporters. The former are integral membrane proteins, sometimes complexes of proteins, which form a regulated hole through the membrane. This regulation, or 'gating' is generally specific to the molecules to be transported by the pore or channel, rendering these transmembrane constructs selectively permeable to a specific class of substrates; for example, a potassium channel is constructed such that only ions having a like charge and size to that of potassium may pass through. Channel and pore proteins tend to have discrete WO 01/00805 PCT/IBOO/00926 -19hydrophobic and hydrophilic domains, such that the hydrophobic face of the protein may associate with the interior of the membrane while the hydrophilic face lines the interior of the channel, thus providing a sheltered hydrophilic environment through which the selected hydrophilic molecule may pass. Many such pores/channels are known in the art, including those for potassium, calcium, sodium, and chloride ions.

This pore and channel-mediated system of facilitated diffusion is limited to very small molecules, such as ions, because pores or channels large enough to permit the passage of whole proteins by facilitated diffusion would be unable to prevent the passage of smaller hydrophilic molecules as well. Transport of molecules by this process is sometimes termed 'facilitated diffusion' since the driving force of a concentration gradient is required for the transport to occur. Permeases also permit facilitated diffusion of larger molecules, such as glucose or other sugars, into the cell when the concentration of these molecules on one side of the membrane is greater than that on the other (also called 'uniport'). In contrast to pores or channels, these integral membrane proteins (often having between 6-14 membrane-spanning a-helices) do not form open channels through the membrane, but rather bind to the target molecule at the surface of the membrane and then undergo a conformational shift such that the target molecule is released on the opposite side of the membrane.

However, cells frequently require the import or export of molecules against the existing concentration gradient ('active transport'), a situation in which facilitated diffusion cannot occur. There are two general mechanisms used by cells for such membrane transport: symport or antiport, and energy-coupled transport such as that mediated by the ABC transporters. Symport and antiport systems couple the movement of two different molecules across the membrane (via permeases having two separate binding sites for the two different molecules); in symport, both molecules are transported in the same direction, while in antiport, one molecule is imported while the other is exported. This is possible energetically because one of the two molecules moves in accordance with a concentration gradient, and this energetically favorable event is permitted only upon concomitant movement of a desired compound against the prevailing concentration gradient. Single molecules may be transported across the membrane against the concentration gradient in an energy-driven process, such as that utilized by the ABC transporters. In this system, the transport protein located in the WO 01/00805 PCT/IB00/00926 membrane has an ATP-binding cassette; upon binding of the target molecule, the ATP is converted to ADP Pi, and the resulting release of energy is used to drive the movement of the target molecule to the opposite face of the membrane, facilitated by the transporter. For more detailed descriptions of all of these transport systems, see: Bamberg, E. et al., (1993) "Charge transport of ion pumps on lipid bilayer membranes", Q. Rev. Biophys. 26: 1-25; Findlay, J.B.C. (1991) "Structure and function in membrane transport systems", Curr. Opin. Struct. Biol. 1:804-810; Higgins, C.F. (1992) "ABC transporters from microorganisms to man", Ann. Rev. Cell Biol. 8: 67-113; Gennis, R.B.

(1989) "Pores, Channels and Transporters", in: Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p. 270-322; and Nikaido, H. and Saier, H. (1992) "Transport proteins in bacteria: common themes in their design", Science 258: 936-942, and references contained within each of these references.

The synthesis of membranes is a well-characterized process involving a number of components, the most important of which are lipid molecules. Lipid synthesis may be divided into two parts: the synthesis of fatty acids and their attachment to snglycerol-3-phosphate, and the addition or modification of a polar head group. Typical lipids utilized in bacterial membranes include phospholipids, glycolipids, sphingolipids, and phosphoglycerides. Fatty acid synthesis begins with the conversion of acetyl CoA either to malonyl CoA by acetyl CoA carboxylase, or to acetyl-ACP by acetyltransacylase. Following a condensation reaction, these two product molecules together form acetoacetyl-ACP, which is converted by a series of condensation, reduction and dehydration reactions to yield a saturated fatty acid molecule having a desired chain length. The production of unsaturated fatty acids from such molecules is catalyzed by specific desaturases either aerobically, with the help of molecular oxygen, or anaerobically (for reference on fatty acid synthesis, see F.C. Neidhardt el al. (1996) E. coli and Salmonella. ASM Press: Washington, p. 612-636 and references contained therein; Lengeler et al. (eds) (1999) Biology of Procaryotes. Thieme: Stuttgart, New York, and references contained therein; and Magnuson, K. et al., (1993) Microbiological Reviews 57: 522-542, and references contained therein). The cyclopropane fatty acids (CFA) are synthesized by a specific CFA-synthase using SAM as a cosubstrate. Branched chain fatty acids are synthesized from branched chain amino acids that are deaminated to yield branched chain 2-oxo-acids (see Lengeler et al., eds.

WO 01/00805 PCT/IB00/00926 -21 (1999) Biology of Procaryotes. Thieme: Stuttgart, New York, and references contained therein). Another essential step in lipid synthesis is the transfer of fatty acids onto the polar head groups by, for example, glycerol-phosphate-acyltransferases. The combination of various precursor molecules and biosynthetic enzymes results in the production of different fatty acid molecules, which has a profound effect on the composition of the membrane.

III. Elements and Methods of the Invention The present invention is based, at least in part, on the discovery of novel molecules, referred to herein as MCT nucleic acid and protein molecules, which control the production of cellular membranes in C. glutamicum and govern the movement of molecules across such membranes. In one embodiment, the MCT molecules participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes. In a preferred embodiment, the activity of the MCT molecules of the present invention to regulate membrane component production and membrane transport has an impact on the production of a desired fine chemical by this organism. In a particularly preferred embodiment, the MCT molecules of the invention are modulated in activity, such that the C. glutamicum metabolic pathways which the MCT proteins of the invention regulate are modulated in yield, production, and/or efficiency of production and the transport of compounds through the membranes is altered in efficiency, which either directly or indirectly modulates the yield, production, and/or efficiency of production of a desired fine chemical by C. glutamicum.

The language, "MCT protein" or "MCT polypeptide" includes proteins which participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes.

Examples of MCT proteins include those encoded by the MCT genes set forth in Table 1 and by the odd-numbered SEQ ID NOs. The terms "MCT gene" or "MCT nucleic acid sequence" include nucleic acid sequences encoding an MCT protein, which consist of a coding region and also corresponding untranslated 5' and 3' sequence regions.

Examples of MCT genes include those set forth in Table 1. The terms "production" or "productivity" are art-recognized and include the concentration of the fermentation WO 01/00805 PCT/IBOO/00926 -22product (for example, the desired fine chemical) formed within a given time and a given fermentation volume kg product per hour per liter). The term "efficiency of production" includes the time required for a particular level of production to be achieved (for example, how long it takes for the cell to attain a particular rate of output of a fine chemical). The term "yield" or "product/carbon yield" is art-recognized and includes the efficiency of the conversion of the carbon source into the product fine chemical). This is generally written as, for example, kg product per kg carbon source.

By increasing the yield or production of the compound, the quantity of recovered molecules, or of useful recovered molecules of that compound in a given amount of culture over a given amount of time is increased. The terms "biosynthesis" or a "biosynthetic pathway" are art-recognized and include.the synthesis of a compound, preferably an organic compound, by a cell from intermediate compounds in what may be a multistep and highly regulated process. The terms "degradation" or a "degradation pathway" are art-recognized and include the breakdown of a compound, preferably an organic compound, by a cell to degradation products (generally speaking, smaller or less complex molecules) in what may be a multistep and highly regulated process. The language "metabolism" is art-recognized and includes the totality of the biochemical reactions that take place in an organism. The metabolism of a particular compound, then, the metabolism of an amino acid such as glycine) comprises the overall biosynthetic, modification, and degradation pathways in the cell related to this compound.

In another embodiment, the MCT molecules of the invention are capable of modulating the production of a desired molecule, such as a fine chemical, in a microorganism such as C. glutamicum. There are a number of mechanisms by which the alteration of an MCT protein of the invention may directly affect the yield, production, and/or efficiency of production of a fine chemical from a C. glutamicum strain incorporating such an altered protein. Those MCT proteins involved in the export of fine chemical molecules from the cell may be increased in number or activity such that greater quantities of these compounds are secreted to the extracellular medium, from which they are more readily recovered. Similarly, those MCT proteins involved in the import of nutrients necessary for the biosynthesis of one or more fine chemicals phosphate, sulfate, nitrogen compounds, etc.) may be increased in number or WO 01/00805 PCT/IB00/00926 -23activity such that these precursor, cofactor, or intermediate compounds are increased in concentration within the cell. Further, fatty acids and lipids themselves are desirable fine chemicals; by optimizing the activity or increasing the number of one or more MCT proteins of the invention which participate in the biosynthesis of these compounds, or by impairing the activity of one or more MCT proteins which are involved in the degradation of these compounds, it may be possible to increase the yield, production, and/or efficiency of production of fatty acid and lipid molecules from C glutamicum.

The mutagenesis of one or more MCT genes of the invention may also result in MCT proteins having altered activities which indirectly impact the production of one or more desired fine chemicals from C .glutamicum. For example, MCT proteins of the invention involved in the export of waste products may be increased in number or activity such that the normal metabolic wastes of the cell (possibly increased in quantity due to the overproduction of the desired fine chemical) are efficiently exported before they are able to damage nucleotides and proteins within the cell (which would decrease the viability of the cell) or to interfere with fine chemical biosynthetic pathways (which would decrease the yield, production, or efficiency of production of the desired fine chemical). Further, the relatively large intracellular quantities of the desired fine chemical may in itself be toxic to the cell, so by increasing the activity or number of transporters able to export this compound from the cell, one may increase the viability of the cell in culture, in turn leading to a greater number of cells in the culture producing the desired fine chemical. The MCT proteins of the invention may also be manipulated such that the relative amounts of different lipid and fatty acid molecules are produced.

This may have a profound effect on the lipid composition of the membrane of the cell.

Since each type of lipid has different physical properties, an alteration in the lipid composition of a membrane may significantly alter membrane fluidity. Changes in membrane fluidity can impact the transport of molecules across the membrane, as well as the integrity of the cell, both of which have a profound effect on the production of fine chemicals from C. glutamicum in large-scale fermentative culture.

The isolated nucleic acid sequences of the invention are contained within the genome of a Corynebacterium glutamicum strain available through the American Type Culture Collection, given designation ATCC 13032. The nucleotide sequence of the isolated C glutamicum MCT DNAs and the predicted amino acid sequences of the C.

WO 01/00805 PCT/IB00/00926 -24glutamicum MCT proteins are shown in the Sequence Listing as odd-numbered SEQ ID NOs and even-numbered SEQ ID NOs, respectively. Computational analyses were performed which classified and/or identified these nucleotide sequences as sequences which encode proteins involved in the metabolism of cellular membrane components or proteins involved in the transport of compounds across such membranes.

The present invention also pertains to proteins which have an amino acid sequence which is substantially homologous to an amino acid sequence of the invention the sequence of an even-numbered SEQ ID NO of the Sequence Listing).. As used herein, a protein which has an amino acid sequence which is substantially homologous to a selected amino acid sequence is least about 50% homologous to the selected amino acid sequence, the entire selected amino acid sequence. A protein which has an amino acid sequence which is substantially homologous to a selected amino acid sequence can also be least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, or 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to the selected amino acid sequence.

The MCT protein or a biologically active portion or fragment thereof of the invention can participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes, or have one or more of the activities set forth in Table 1.

Various aspects of the invention are described in further detail in the following subsections: A. Isolated Nucleic Acid Molecules One aspect of the invention pertains to isolated nucleic acid molecules that encode MCT polypeptides or biologically active portions thereof, as well as nucleic acid fragments sufficient for use as hybridization probes or primers for the identification or amplification of MCT-encoding nucleic acid MCT DNA). As used herein, the term "nucleic acid molecule" is intended to include DNA molecules cDNA or genomic DNA) and RNA molecules mRNA) and analogs of the DNA or RNA generated using nucleotide analogs. This term also encompasses untranslated sequence located at both the 3' and 5' ends of the coding region of the gene: at least about 100 nucleotides of sequence upstream from the 5' end of the coding region and at least about WO 01/00805 PCT/IB00/00926 nucleotides of sequence downstream from the 3'end of the coding region of the gene.

The nucleic acid molecule can be single-stranded or double-stranded, but preferably is double-stranded DNA. An "isolated" nucleic acid molecule is one which is separated from other nucleic acid molecules which are present in the natural source of the nucleic acid. Preferably, an "isolated" nucleic acid is free of sequences which naturally flank the nucleic acid sequences located at the 5' and 3' ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. For example, in various embodiments, the isolated MCT nucleic acid molecule can contain less than about 5 kb, 4kb, 3kb, 2kb, 1 kb, 0.5 kb or 0.1 kb of nucleotide sequences which naturally flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived a C. glutamicum cell). Moreover, an "isolated" nucleic acid molecule, such as a DNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized.

A nucleic acid molecule of the present invention, a nucleic acid molecule having a nucleotide sequence of an odd-numbered SEQ ID NO of the Sequence Listing, or a portion thereof, can be isolated using standard molecular biology techniques and the sequence information provided herein. For example, a C glutamicum MCT DNA can be isolated from a C. glutamicum library using all or portion of one of the odd-numbered SEQ ID NO sequences of the Sequence Listing as a hybridization probe and standard hybridization techniques as described in Sambrook, Fritsh, E. and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989).

Moreover, a nucleic acid molecule encompassing all or a portion of one of the nucleic acid sequences of the invention an odd-numbered SEQ ID NO:) can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this sequence a nucleic acid molecule encompassing all or a portion of one of the nucleic acid sequences of the invention an odd-numbered SEQ ID NO of the Sequence Listing) can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this same sequence). For example, mRNA can be isolated from normal endothelial cells by the guanidinium-thiocyanate extraction procedure of Chirgwin et al. (1979) Biochemistry 18: 5294-5299) and DNA WO 01/00805 PCT/IB00/00926 -26can be prepared using reverse transcriptase Moloney MLV reverse transcriptase, available from Gibco/BRL, Bethesda, MD; or AMV reverse transcriptase, available from Seikagaku America, Inc., St. Petersburg, FL). Synthetic oligonucleotide primers for polymerase chain reaction amplification can be designed based upon one of the nucleotide sequences shown in the Sequence Listing. A nucleic acid of the invention can be amplified using cDNA or, alternatively, genomic DNA, as a template and appropriate oligonucleotide primers according to standard PCR amplification techniques. The nucleic acid so amplified can be cloned into an appropriate vector and characterized by DNA sequence analysis. Furthermore, oligonucleotides corresponding to an MCT nucleotide sequence can be prepared by standard synthetic techniques, e.g., using an automated DNA synthesizer.

In a preferred embodiment, an isolated nucleic acid molecule of the invention comprises one of the nucleotide sequences shown in the Sequence Listing. The nucleic acid sequences of the invention, as set forth in the Sequence Listing correspond to the Corynebacterium glutamicum MCT DNAs of the invention. This DNA comprises sequences encoding MCT proteins the "coding region", indicated in each oddnumbered SEQ ID NO: in the Sequence Listing), as well as 5' untranslated sequences and 3' untranslated sequences, also indicated in each odd-numbered SEQ ID NO: in the Sequence Listing. Alternatively, the nucleic acid molecule can comprise only the coding region of any of the nucleic acid sequences of the Sequence Listing.

For the purposes of this application, it will be understood that each of the nucleic acid and amino acid sequences set forth in the Sequence Listing has an identifying RXA, RXN, RXS, or RXC number having the designation "RXA", "RXN", "RXS" or "RXC" followed by 5 digits RXA02099, RXN03097, RXS00148, or RXC01748). Each of the nucleic acid sequences comprises up to three parts: a 5' upstream region, a coding region, and a downstream region. Each of these three regions is identified by the same RXA, RXN, RXS, or RXC designation to eliminate confusion. The recitation "one of the odd-numbered sequences in of the Sequence Listing", then, refers to any of the nucleic acid sequences in the Sequence Listing, which may also be distinguished by their differing RXA, RXN, RXS, or RXC designations. The coding region of each of these sequences is translated into a corresponding amino acid sequence, which is also set forth in the Sequence Listing, as an even-numbered SEQ ID NO: immediately following WO 01/00805 PCT/IB00/00926 -27the corresponding nucleic acid sequence. For example, the coding region for RXA03097 is set forth in SEQ ID NO:1, while the amino acid sequence which it encodes is set forth as SEQ ID NO:2. The sequences of the nucleic acid molecules of the invention are identified by the same RXA, RXN, RXS, or RXC designations as the amino acid molecules which they encode, such that they can be readily correlated. For example, the amino acid sequences designated RXA02099, RXN03097, RXS00148, and RXC01748 are translations of the coding region of the nucleotide sequences of nucleic acid molecules RXA02099, RXN03097, RXS00148, and RXC01748, respectively. The correspondence between the RXA, RXN, RXS, and RXC nucleotide and amino acid sequences of the invention and their assigned SEQ ID NOs is set forth in Table 1. For example, as set forth in Table 1, the nucleotide sequence of RXA00104 is SEQ ID and the amino acid sequence of RXA00104 is SEQ ID NO:6.

Several of the genes of the invention are "F-designated genes". An F-designated gene includes those genes set forth in Table 1 which have an in front of the RXA, RXN, RXS, or RXC designation. For example, SEQ ID NO:11, designated, as indicated on Table 1, as "F RXA02581", is an F-designated gene, as are SEQ ID NOs: 31, 33, and 43 (designated on Table 1 as "F RXA02487", "F RXA02490", and "F RXA02809", respectively).

In one embodiment, the nucleic acid molecules of the present invention are not intended to include those compiled in Table 2. In the case of the dapD gene, a sequence for this gene was published in Wehrmann, et al. (1998) J. Bacteriol. 180(12): 3159- 3165. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.

In another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleic acid molecule which is a complement of one of the nucleotide sequences of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing), or a portion thereof. A nucleic acid molecule which is complementary to one of the nucleotide sequences shown in of the invention is one which is sufficiently complementary to one of the nucleotide sequences shown in the Sequence Listing the sequence of an odd-numbered SEQ ID NO:)such that it can WO 01/00805 PCT/IB00/00926 -28hybridize to one of the nucleotide sequences of the invention, thereby forming a stable duplex.

In still another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing), or a portion thereof. Ranges and identity values intermediate to the above-recited ranges, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. In an additional preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes, e.g., hybridizes under stringent conditions, to one of the nucleotide sequences of the invention, or a portion thereof.

Moreover, the nucleic acid molecule of the invention can comprise only a portion of the coding region of the sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, for example a fragment which can be used as a probe or primer or a fragment encoding a biologically active portion of an MCT protein. The nucleotide sequences determined from the cloning of the MCT genes from C. glutamicum allows for the generation of probes and primers designed for use in identifying and/or cloning MCT homologues in other cell types and organisms, as well as MCT homologues from other Corynebacteria or related species. The probe/primer typically comprises substantially purified oligonucleotide. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, preferably about 25, more preferably about 40, 50 or 75 consecutive nucleotides of a sense strand of one of the nucleotide sequences of the invention a sequence of one of the odd-numbered SEQ 1D NOs of the Sequence Listing), an anti-sense sequence of one of these sequences, or naturally occurring mutants thereof. Primers based on a WO 01/00805 PCT/IB00/00926 -29nucleotide sequence of the invention can be used in PCR reactions to clone MCT homologues. Probes based on the MCT nucleotide sequences can be used to detect transcripts or genomic sequences encoding the same or homologous proteins. In preferred embodiments, the probe further comprises a label group attached thereto, e.g.

the label group can be a radioisotope, a fluorescent compound, an enzyme, or an enzyme co-factor. Such probes can be used as a part of a diagnostic test kit for identifying cells which misexpress an MCT protein, such as by measuring a level of an MCT-encoding nucleic acid in a sample of cells, detecting MCT mRNA levels or determining whether a genomic MCT gene has been mutated or deleted.

In one embodiment, the nucleic acid molecule of the invention encodes a protein or portion thereof which includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence of an evennumbered SEQ ID NO of the Sequence Listing) such that the protein or portion thereof maintains the ability to participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes. As used herein, the language "sufficiently homologous" refers to proteins or portions thereof which have amino acid sequences which include a minimum number of identical or equivalent an amino acid residue which has a similar side chain as an amino acid residue in a sequence of one of the even-numbered SEQ ID NOs of the Sequence Listing) amino acid residues to an amino acid sequence of the invention such that the protein or portion thereof is able to participate in the metabolism of compounds necessary for the construction of cellular membranes in C.

glutamicum, or in the transport of molecules across these membranes. Protein members of such membrane component metabolic pathways or membrane transport systems, as described herein, may play a role in the production and secretion of one or more fine chemicals. Examples of such activities are also described herein. Thus, "the function of an MCT protein" contributes either directly or indirectly to the yield, production, and/or efficiency of production of one or more fine chemicals. Examples of MCT protein activities are set forth in Table 1.

In another embodiment, the protein is at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to an entire amino WO 01/00805 PCT/IB00/00926 acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing).

Portions of proteins encoded by the MCT nucleic acid molecules of the invention are preferably biologically active portions of one of the MCT proteins. As used herein, the term "biologically active portion of an MCT protein" is intended to include a portion, a domain/motif, of an MCT protein that participates in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes, or has an activity as set forth in Table 1. To determine whether an MCT protein or a biologically active portion thereof can participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes, an assay of enzymatic activity may be performed. Such assay methods are well known to those of ordinary skill in the art, as detailed in Example 8 of the Exemplification.

Additional nucleic acid fragments encoding biologically active portions of an MCT protein can be prepared by isolating a portion of one of the amino acid sequences of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing), expressing the encoded portion of the MCT protein or peptide by recombinant expression in vitro) and assessing the activity of the encoded portion of the MCT protein or peptide.

The invention further encompasses nucleic acid molecules that differ from one of the nucleotide sequences shown of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing) (and portions thereof) due to degeneracy of the genetic code and thus encode the same MCT protein as that encoded by the nucleotide sequences of the invention. In another embodiment, an isolated nucleic acid molecule of the invention has a nucleotide sequence encoding a protein having an amino acid sequence shown in the Sequence Listing an even-numbered SEQ ID In a still further embodiment, the nucleic acid molecule of the invention encodes a full length C. glutamicum protein which is substantially homologous to an amino acid sequence of the invention (encoded by an open reading frame shown in an odd-numbered SEQ ID NO: of the Sequence Listing).

WO 01/00805 PCT/IB00/00926 -31 It will be understood by one of ordinary skill in the art that in one embodiment the sequences of the invention are not meant to include the sequences of the prior art, such as those Genbank sequences set forth in Tables 2 or 4 which were available prior to the present invention. In one embodiment, the invention includes nucleotide and amino acid sequences having a percent identity to a nucleotide or amino acid sequence of the invention which is greater than that of a sequence of the prior art a Genbank sequence (or the protein encoded by such a sequence) set forth in Tables 2 or For example, the invention includes a nucleotide sequence which is greater than and/or at least 38% identical to the nucleotide sequence designated RXA01420 (SEQ ID NO:7), a nucleotide sequence which is greater than and/or at least 43% identical to the nucleotide sequence designated RXA00104 (SEQ ID NO:5), and a nucleotide sequence which is greater than and/or at least 45% identical to the nucleotide sequence designated RXA02173 (SEQ ID NO:25). One of ordinary skill in the art would be able to calculate the lower threshold of percent identity for any given sequence of the invention by examining the GAP-calculated percent identity scores set forth in Table 4 for each of the three top hits for the given sequence, and by subtracting the highest GAP-calculated percent identity from 100 percent. One of ordinary skill in the art will also appreciate that nucleic acid and amino acid sequences having percent identities greater than the lower threshold so calculated at least 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more identical) are also encompassed by the invention.

In addition to the C. glutamicum MCT nucleotide sequences set forth in the Sequence Listing as odd-numbered SEQ ID NOs, it will be appreciated by one of ordinary skill in the art that DNA sequence polymorphisms that lead to changes in the amino acid sequences of MCT proteins may exist within a population the C.

glutamicum population). Such genetic polymorphism in the MCT gene may exist among individuals within a population due to natural variation. As used herein, the terms "gene" and "recombinant gene" refer to nucleic acid molecules comprising an open reading frame encoding an MCT protein, preferably a C. glutamicum MCT protein.

WO 01/00805 PCT/IBOO/00926 -32- Such natural variations can typically result in 1-5% variance in the nucleotide sequence of the MCT gene. Any and all such nucleotide variations and resulting amino acid polymorphisms in MCT that are the result of natural variation and that do not alter the functional activity of MCT proteins are intended to be within the scope of the invention.

Nucleic acid molecules corresponding to natural variants and non-C glutamicum homologues of the C. glutamicum MCT DNA of the invention can be isolated based on their homology to the C. glutamicum MCT nucleic acid disclosed herein using the C.

glutamicum DNA, or a portion thereof, as a hybridization probe according to standard hybridization techniques under stringent hybridization conditions. Accordingly, in another embodiment, an isolated nucleic acid molecule of the invention is at least nucleotides in length and hybridizes under stringent conditions to the nucleic acid molecule comprising a nucleotide sequence of an odd-numbered SEQ ID NO: of the Sequence Listing. In other embodiments, the nucleic acid is at least 30, 50, 100, 250 or more nucleotides in length. As used herein, the term "hybridizes under stringent conditions" is intended to describe conditions for hybridization and washing under which nucleotide sequences at least 60% homologous to each other typically remain hybridized to each other. Preferably, the conditions are such that sequences at least about 65%, more preferably at least about 70%, and even more preferably at least about or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to those of ordinary skill in the art and can be found in Current Protocols in Molecular Biology, John Wiley Sons, N.Y. (1989), 6.3.1-6.3.6.

A preferred, non-limiting example of stringent hybridization conditions are hybridization in 6X sodium chloride/sodium citrate (SSC) at about 451C, followed by one or more washes in 0.2 X SSC, 0. 1% SDS at 50-651C. Preferably, an isolated nucleic acid molecule of the invention that hybridizes under stringent conditions to a nucleotide sequence of the invention corresponds to a naturally-occurring nucleic acid molecule. As used herein, a "naturally-occurring" nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature encodes a natural protein). In one embodiment, the nucleic acid encodes a natural C.

glutamicum MCT protein.

In addition to naturally-occurring variants of the MCT sequence that may exist in the population, one of ordinary skill in the art will further appreciate that changes can be WO 01/00805 PCT/IB00/00926 -33 introduced by mutation into a nucleotide sequence of the invention, thereby leading to changes in the amino acid sequence of the encoded MCT protein, without altering the functional ability of the MCT protein. For example, nucleotide substitutions leading to amino acid substitutions at "non-essential" amino acid residues can be made in a nucleotide sequence of the invention. A "non-essential" amino acid residue is a residue that can be altered from the wild-type sequence of one of the MCT proteins an even-numbered SEQ ID NO: of the Sequence Listing) without altering the activity of said MCT protein, whereas an "essential" amino acid residue is required for MCT protein activity. Other amino acid residues, however, those that are not conserved or only semi-conserved in the domain having MCT activity) may not be essential for activity and thus are likely to be amenable to alteration without altering MCT activity.

Accordingly, another aspect of the invention pertains to nucleic acid molecules encoding MCT proteins that contain changes in amino acid residues that are not essential for MCT activity. Such MCT proteins differ in amino acid sequence from a sequence of an even-numbered SEQ ID NO: of the Sequence Listing yet retain at least one of the MCT activities described herein. In one embodiment, the isolated nucleic acid molecule comprises a nucleotide sequence encoding a protein, wherein the protein comprises an amino acid sequence at least about 50% homologous to an amino acid sequence of the invention and is capable of participate in the metabolism of compounds necessary for the construction of cellular membranes iifi C. glutamicum, or in the transport of molecules across these membranes, or has one or more activities set forth in Table 1. Preferably, the protein encoded by the nucleic acid molecule is at least about 50-60% homologous to the amino acid sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, more preferably at least about 60-70% homologous to one of these sequences, even more preferably at least about 70-80%, 80-90%, 90-95% homologous to one of these sequences, and most preferably at least about 96%, 97%, 98%, or 99% homologous to one of the amino acid sequences of the invention..

To determine the percent homology of two amino acid sequences one of the amino acid sequences of the invention and a mutant form thereof) or of two nucleic acids, the sequences are aligned for optimal comparison purposes gaps can be introduced in the sequence of one protein or nucleic acid for optimal alignment with the other protein or nucleic acid). The amino acid residues or nucleotides at corresponding WO 01/00805 PCT/IB00/00926 -34amino acid positions or nucleotide positions are then compared. When a position in one sequence one of the amino acid sequences of the invention) is occupied by the same amino acid residue or nucleotide as the corresponding position in the other sequence a mutant form of the amino acid sequence), then the molecules are homologous at that position as used herein amino acid or nucleic acid "homology" is equivalent to amino acid or nucleic acid "identity"). The percent homology between the two sequences is a function of the number of identical positions shared by the sequences homology of identical positions/total of positions x 100).

An isolated nucleic acid molecule encoding an MCT protein homologous to a protein sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) can be created by introducing one or more nucleotide substitutions, additions or deletions into a nucleotide sequence of the invention such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein. Mutations can be introduced into one of the nucleotide sequences of the invention by standard techniques, such as site-directed mutagenesis and PCRmediated mutagenesis. Preferably, conservative amino acid substitutions are made at one or more predicted non-essential amino acid residues. A "conservative amino acid substitution" is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains lysine, arginine, histidine), acidic side chains aspartic acid, glutamic acid), uncharged polar side chains glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains threonine, valine, isoleucine) and aromatic side chains tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue in an MCT protein is preferably replaced with another amino acid residue from the same side chain family. Alternatively, in another embodiment, mutations can be introduced randomly along all or part of an MCT coding sequence, such as by saturation mutagenesis, and the resultant mutants can be screened for an MCT activity described herein to identify mutants that retain MCT activity. Following mutagenesis of the nucleotide sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, WO 01/00805 PCTIB00/00926 the encoded protein can be expressed recombinantly and the activity of the protein can be determined using, for example, assays described herein (see Example 8 of the Exemplification).

In addition to the nucleic acid molecules encoding MCT proteins described above, another aspect of the invention pertains to isolated nucleic acid molecules which are antisense thereto. An "antisense" nucleic acid comprises a nucleotide sequence which is complementary to a "sense" nucleic acid encoding a protein, e.g., complementary to the coding strand of a double-stranded cDNA molecule or complementary to an mRNA sequence. Accordingly, an antisense nucleic acid can hydrogen bond to a sense nucleic acid. The antisense nucleic acid can be complementary to an entire MCT coding strand, or to only a portion thereof. In one embodiment, an antisense nucleic acid molecule is antisense to a "coding region" of the coding strand of a nucleotide sequence encoding an MCT protein. The term "coding region" refers to the region of the nucleotide sequence comprising codons which are translated into amino acid residues the entire coding region of SEQ ID (RXA00104) comprises nucleotides 1 to 756). In another embodiment, the antisense nucleic acid molecule is antisense to a "noncoding region" of the coding strand of a nucleotide sequence encoding MCT. The term "noncoding region" refers to 5' and 3' sequences which flank the coding region that are not translated into amino acids also referred to as 5' and 3' untranslated regions).

Given the coding strand sequences encoding MCT disclosed herein the sequences set forth as odd-numbered SEQ ID NOs in the Sequence Listing), antisense nucleic acids of the invention can be designed according to the rules of Watson and Crick base pairing. The antisense nucleic acid molecule can be complementary to the entire coding region of MCT mRNA, but more preferably is an oligonucleotide which is antisense to only a portion of the coding or noncoding region of MCT mRNA. For example, the antisense oligonucleotide can be complementary to the region surrounding the translation start site of MCT mRNA. An antisense oligonucleotide can be, for example, about 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50 nucleotides in length. An antisense nucleic acid of the invention can be constructed using chemical synthesis and enzymatic ligation reactions using procedures known in the art. For example, an antisense nucleic acid an antisense oligonucleotide) can be chemically synthesized WO 01/00805 PCT/IB00/00926 -36using naturally occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids, phosphorothioate derivatives and acridine substituted nucleotides can be used. Examples of modified nucleotides which can be used to generate the antisense nucleic acid include fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4acetylcytosine, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethyl-2thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-Dgalactosylqueosine, inosine, N6-isopentenyladenine, I-methylguanine, 1-methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, acid wybutoxosine, pseudouracil, queosine, 2-thiocytosine, methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5- oxyacetic acid methylester, uracil-5-oxyacetic acid 5-methyl-2-thiouracil, 3-(3-amino-3-N-2carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine. Alternatively, the antisense nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).

The antisense nucleic acid molecules of the invention are typically administered to a cell or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encoding an MCT protein to thereby inhibit expression of the protein, by inhibiting transcription and/or translation. The hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule which binds to DNA duplexes, through specific interactions in the major groove of the double helix. The antisense molecule can be modified such that it specifically binds to a receptor or an antigen expressed on a selected cell surface, by linking the antisense nucleic acid molecule to a peptide or an antibody which binds to a cell surface receptor or antigen. The antisense nucleic acid molecule can also be delivered to cells using the vectors described herein. To achieve WO 01/00805 PCT/IB00/00926 -37sufficient intracellular concentrations of the antisense molecules, vector constructs in which the antisense nucleic acid molecule is placed under the control of a strong prokaryotic, viral, or eukaryotic promoter are preferred.

In yet another embodiment, the antisense nucleic acid molecule of the invention is an a-anomeric nucleic acid molecule. An a-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual p-units, the strands run parallel to each other (Gaultier et al. (1987) Nucleic Acids.

Res. 15:6625-6641). The antisense nucleic acid molecule can also comprise a 2'-omethylribonucleotide (Inoue et al. (1987) Nucleic Acids Res. 15:6131-6148) or a chimeric RNA-DNA analogue (Inoue et al. (1987) FEBS Lett. 215:327-330).

In still another embodiment, an antisense nucleic acid of the invention is a ribozyme. Ribozymes are catalytic RNA molecules with ribonuclease activity which are capable of cleaving a single-stranded nucleic acid, such as an mRNA, to which they have a complementary region. Thus, ribozymes hammerhead ribozymes (described in Haselhoff and Gerlach (1988) Nature 334:585-591)) can be used to catalytically cleave MCT mRNA transcripts to thereby inhibit translation of MCT mRNA. A ribozyme having specificity for an MCT-encoding nucleic acid can be designed based upon the nucleotide sequence of an MCT DNA disclosed herein SEQ ID NO. 5 (RXA00104). For example, a derivative of a Tetrahymena L-19 IVS RNA can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in an MCT-encoding mRNA.

See, e.g, Cech et al. U.S. Patent No. 4,987,071 and Cech et al. U.S. Patent No.

5,116,742. Alternatively, MCT mRNA can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules. See, Bartel, D. and Szostak, J.W. (1993) Science 261:1411-1418.

Alternatively, MCT gene expression can be inhibited by targeting nucleotide sequences complementary to the regulatory region of an MCT nucleotide sequence an MCT promoter and/or enhancers) to form triple helical structures that prevent transcription of an MCT gene in target cells. See generally, Helene, C. (1991) Anticancer Drug Des. 6(6):569-84; Helene, C. et al. (1992) Ann. N. Y. Acad. Sci. 660:27- 36; and Maher, L.J. (1992) Bioassays 14(12):807-15.

WO 01/00805 PCTIBO00/00926 -38- B. Recombinant Expression Vectors and Host Cells Another aspect of the invention pertains to vectors, preferably expression vectors, containing a nucleic acid encoding an MCT protein (or a portion thereof). As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a "plasmid", which refers to a circular double stranded DNA loop into which additional DNA segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as "expression vectors". In general, expression vectors of utility in recombinant DNA techniques are often in the form of plasmids. In the present specification, "plasmid" and "vector" can be used interchangeably as the plasmid is the most commonly used form of vector.

However, the invention is intended to include such other forms of expression vectors, such as viral vectors replication defective retroviruses, adenoviruses and adenoassociated viruses), which serve equivalent functions.

The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for.expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, which is operatively linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, "operably linked" is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner which allows for expression of the nucleotide sequence in an in vitro transcription/translation system or in a host cell when the vector is introduced into the host cell). The term "regulatory sequence" is intended to include promoters, enhancers and other expression control elements polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel; Gene Expression Technology: Methods in Enzymology 185, WO 01/00805 PCT/IB00/00926 -39- Academic Press, San Diego, CA (1990). Regulatory sequences include those which direct constitutive expression of a nucleotide sequence in many types of host cell and those which direct expression of the nucleotide sequence only in certain host cells.

Preferred regulatory sequences are, for example, promoters such as cos-, tac-, trp-, tet-, trp-tet-, Ipp-, lac-, Ipp-lac-, lacI q T7-, T5-, T3-, gal-, trc-, ara-, SP6-, amy, SPO2, X-PRor X PL, which are used preferably in bacteria. Additional regulatory sequences are, for example, promoters from yeasts and fungi, such as ADC1, MFa, AC, P-60, CYC 1, GAPDH, TEF, rp28, ADH, promoters from plants such as CaMV/35S, SSU, OCS, lib4, usp, STLSI, B33, nos or ubiquitin- or phaseolin-promoters. It is also possible to use artificial promoters. It will be appreciated by one of ordinary skill in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein MCT proteins, mutant forms of MCT proteins, fusion proteins, etc.).

The recombinant expression vectors of the invention can be designed for expression of MCT proteins in prokaryotic or eukaryotic cells. For example, MCT genes can be expressed in bacterial cells such as C. glutamicum, insect cells (using baculovirus expression vectors), yeast and other fungal cells (see Romanos, M.A. et al.

(1992) "Foreign gene expression in yeast: a review", Yeast 8: 423-488; van den Hondel, C.A.M.J.J. et al. (1991) "Heterologous gene expression in filamentous fungi" in: More Gene Manipulations in Fungi, J.W. Bennet L.L. Lasure, eds., p. 396-428: Academic Press: San Diego; and van den Hondel, C.A.M.J.J. Punt, P.J. (1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, Peberdy, J.F. et al., eds., p. 1-28, Cambridge University Press: Cambridge), algae and multicellular plant cells (see Schmidt, R. and Willmitzer, L. (1988) High efficiency Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana leaf and cotyledon explants" Plant Cell Rep.: 583-586), or mammalian cells.

Suitable host cells are discussed further in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.

WO 01/00805 PCT/IB00/00926 Expression of proteins in prokaryotes is most often carried out with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion proteins. Fusion vectors add a number of amino acids to a protein encoded therein, usually to the amino terminus of the recombinant protein but also to the C-terminus or fused within suitable regions in the proteins. Such fusion vectors typically serve three purposes: 1) to increase expression of recombinant protein; 2) to increase the solubility of the recombinant protein; and 3) to aid in the purification of the recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant protein to enable separation of the recombinant protein from the fusion moiety subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase.

Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith, D.B. and Johnson, K.S. (1988) Gene 67:31-40), pMAL (New England Biolabs, Beverly, MA) and pRIT5 (Pharmacia, Piscataway, NJ) which fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. In one embodiment, the coding sequence of the MCT protein is cloned into a pGEX expression vector to create a vector encoding a fusion protein comprising, from the N-terminus to the C-terminus, GST-thrombin cleavage site-X protein. The fusion protein can be purified by affinity chromatography using glutathione-agarose resin.

Recombinant MCT protein unfused to GST can be recovered by cleavage of the fusion protein with thrombin.

Examples of suitable inducible non-fusion E. coli expression vectors include pTrc (Amann et al., (1988) Gene 69:301-315) pLG338, pACYC184, pBR322, pUC 18, pUC19, pKC30, pRep4, pHS1, pHS2, pPLc236, pMBL24, pLG200, pUR290, pIN- II11113-B1, Xgtl 1, pBdCl, and pET 1 Id (Studier et al., Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 60-89 and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

Target gene expression from the pTrc vector relies on host RNA polymerase transcription from a hybrid trp-lac fusion promoter. Target gene expression from the pET 1 Id vector relies on transcription from a T7 gnl0-lac fusion promoter mediated by a coexpressed viral RNA polymerase (T7 gnl). This viral polymerase is supplied by WO 01/00805 PCT/IB00/00926 -41host strains BL21(DE3) or HMS174(DE3) from a resident X prophage harboring a T7 gnl gene under the transcriptional control of the lacUV 5 promoter. For transformation of other varieties of bacteria, appropriate vectors may be selected. For example, the plasmids pIJl01, pIJ364, pIJ702 and pIJ361 are known to be useful in transforming Streptomyces, while plasmids pUB 110, pC 194, or pBD214 are suited for transformation of Bacillus species. Several plasmids of use in the transfer of genetic information into Corynebacterium include pHM1519, pBL1, pSA77, or pAJ667 (Pouwels et al., eds.

(1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

One strategy to maximize recombinant protein expression is to express the protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein (Gottesman, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 119-128). Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in the bacterium chosen for expression, such as C. glutamicum (Wada et al. (1992) Nucleic Acids Res. 20:2111-2118). Such alteration of nucleic acid sequences of the invention can be carried out by standard DNA synthesis techniques.

In another embodiment, the MCT protein expression vector is a yeast expression vector. Examples of vectors for expression in yeast S. cerevisiae include pYepSecl (Baldari, et al., (1987) Embo J 6:229-234), 2 p, pAG-1, Yep6, Yepl3, pEMBLYe23, pMFa (Kurjan and Herskowitz, (1982) Cell 30:933-943), pJRY88 (Schultz et al., (1987) Gene 54:113-123), and pYES2 (Invitrogen Corporation, San Diego, CA). Vectors and methods for the construction of vectors appropriate for use in other fungi, such as the filamentous fungi, include those detailed in: van den Hondel, C.A.M.J.J. Punt, P.J.

(1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, J.F. Peberdy, et al., eds., p. 1-28, Cambridge University Press: Cambridge, and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York (IBSN 0 444 904018).

Alternatively, the MCT proteins of the invention can be expressed in insect cells using baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells Sf 9 cells) include the pAc series (Smith et al.

WO 01/00805 PCT/IB00/00926 -42- (1983) Mol. Cell Biol. 3:2156-2165) and the pVL series (Lucklow and Summers (1989) Virology 170:31-39).

In another embodiment, the MCT proteins of the invention may be expressed in unicellular plant cells (such as algae) or in plant cells from higher plants the spermatophytes, such as crop plants). Examples of plant expression vectors include those detailed in: Becker, Kemper, Schell, J. and Masterson, R. (1992) "New plant binary vectors with selectable markers located proximal to the left border", Plant Mol. Biol. 20: 1195-1197; and Bevan, M.W. (1984) "Binary Agrobacterium vectors for plant transformation", Nucl. Acid. Res. 12: 8711-8721, and include pLGV23, pGHlac+, pBIN19, pAK2004, and pDH51 (Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

In yet another embodiment, a nucleic acid of the invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include pCDM8 (Seed, B. (1987) Nature 329:840) and pMT2PC (Kaufman et al. (1987) EMBOJ. 6:187-195). When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements.

For example, commonly used promoters are derived from polyoma, Adenovirus 2, cytomegalovirus and Simian Virus 40. For other suitable expression systems for both prokaryotic and eukaryotic cells see chapters 16 and 17 of Sambrook, Fritsh, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.

In another embodiment, the recombinant mammalian expression vector is capable of directing expression of the nucleic acid preferentially in a particular cell type tissue-specific regulatory elements are used to express the nucleic acid). Tissuespecific regulatory elements are known in the art. Non-limiting examples of suitable tissue-specific promoters include the albumin promoter (liver-specific; Pinkert et al.

(1987) Genes Dev. 1:268-277), lymphoid-specific promoters (Calame and Eaton (1988) Adv. Immunol. 43:235-275), in particular promoters ofT cell receptors (Winoto and Baltimore (1989) EMBOJ. 8:729-733) and immunoglobulins (Banerji et al. (1983) Cell 33:729-740; Queen and Baltimore (1983) Cell 33:741-748), neuron-specific promoters the neurofilament promoter; Byrne and Ruddle (1989) PNAS 86:5473-5477), WO 01/00805 PCTfIB00/00926 -43pancreas-specific promoters (Edlund et al. (1985) Science 230:912-916), and mammary gland-specific promoters milk whey promoter; U.S. Patent No. 4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are also encompassed, for example the murine hox promoters (Kessel and Gruss (1990) Science 249:374-379) and the a-fetoprotein promoter (Campes and Tilghman (1989) Genes Dev. 3:537-546).

The invention further provides a recombinant expression vector comprising a DNA molecule of the invention cloned into the expression vector in an antisense orientation. That is, the DNA molecule is operatively linked to a regulatory sequence in a manner which allows for expression (by transcription of the DNA molecule) of an RNA molecule which is antisense to MCT mRNA. Regulatory sequences operatively linked to a nucleic acid cloned in the antisense orientation can be chosen which direct the continuous expression of the antisense RNA molecule in a variety of cell types, for instance viral promoters and/or enhancers, or regulatory sequences can be chosen which direct constitutive, tissue specific or cell type specific expression of antisense RNA.

The antisense expression vector can be in the form of a recombinant plasmid, phagemid or attenuated virus in which antisense nucleic acids are produced under the control of a high efficiency regulatory region, the activity ofwhich can be determined by the cell type into which the vector is introduced. For a discussion of the regulation of gene expression using antisense genes see Weintraub, H. et al., Antisense RNA as a molecular tool for genetic analysis, Reviews Trends in Genetics, Vol. 1(1) 1986.

Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms "host cell" and "recombinant host cell" are used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.

A host cell can be any prokaryotic or eukaryotic cell. For example, an MCT protein can be expressed in bacterial cells such as C. glutamicum, insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells). Other WO 01/00805 PCT/IB00/00926 -44suitable host cells are known to one of ordinary skill in the art. Microorganisms related to Corynebacterium glutamicum which may be conveniently used as host cells for the nucleic acid and protein molecules of the invention are set forth in Table 3.

Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms "transformation" and "transfection", "conjugation" and "transduction" are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid linear DNA or RNA a linearized vector or a gene construct alone without a vector) or nucleic acid in the form of a vector a plasmid, phage, phasmid, phagemid, transposon or other DNA) into a host cell, including calcium phosphate or calcium chloride co-precipitation, DEAE-dextran-mediated transfection, lipofection, natural competence, chemical-mediated transfer, or electroporation. Suitable methods for transforming or transfecting host cells can be found in Sambrook, et al. (Molecular Cloning: A Laboratory Manual. 2nd, ed, Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989), and other laboratory manuals.

For stable transfection of mammalian cells, it is known that, depending upon the expression vector and transfection technique used, only a small fraction of cells may integrate the foreign DNA into their genome. In order to identify and select these integrants, a gene that encodes a selectable marker resistance to antibiotics) is generally introduced into the host cells along with the gene of interest. Preferred selectable markers include those which confer resistance to drugs, such as G418, hygromycin and methotrexate. Nucleic acid encoding a selectable marker can be introduced into a host cell on the same vector as that encoding an MCT protein or can be introduced on a separate vector. Cells stably transfected with the introduced nucleic acid can be identified by, for example, drug selection cells that have incorporated the selectable marker gene will survive, while the other cells die).

To create a homologous recombinant microorganism, a vector is prepared which contains at least a portion of an MCT gene into which a deletion, addition or substitution has been introduced to thereby alter, functionally disrupt, the MCT gene.

Preferably, this MCT gene is a Corynebacterium glutamicum MCT gene, but it can be a homologue from a related bacterium or even from a mammalian, yeast, or insect source.

In a preferred embodiment, the vector is designed such that, upon homologous WO 01/00805 PCT/IB00/00926 recombination, the endogenous MCT gene is functionally disrupted no longer encodes a functional protein; also referred to as a "knock out" vector). Alternatively, the vector can be designed such that, upon homologous recombination, the endogenous MCT gene is mutated or otherwise altered but still encodes functional protein the upstream regulatory region can be altered to thereby alter the expression of the endogenous MCT protein). In the homologous recombination vector, the altered portion of the MCT gene is flanked at its 5' and 3' ends by additional nucleic acid of the MCT gene to allow for homologous recombination to occur between the exogenous MCT gene carried by the vector and an endogenous MCT gene in a microorganism. The additional flanking MCT nucleic acid is of sufficient length for successful homologous recombination with the endogenous gene. Typically, several kilobases of flanking DNA (both at the 5' and 3' ends) are included in the vector (see Thomas, and Capecchi, M.R. (1987) Cell 51: 503 for a description of homologous recombination vectors). The vector is introduced into a microorganism by electroporation) and cells in which the introduced MCT gene has homologously recombined with the endogenous MCT gene are selected, using art-known techniques.

In another embodiment, recombinant microorganisms can be produced which contain selected systems which allow for regulated expression of the introduced gene.

For example, inclusion of an MCT gene on a vector placing it under control of the lac operon permits expression of the MCT gene only in the presence of IPTG. Such regulatory systems are well known in the art.

In another embodiment, an endogenous MCT gene in a host cell is disrupted by homologous recombination or other genetic means known in the art) such that expression of its protein product does not occur. In another embodiment, an endogenous or introduced MCT gene in a host cell has been altered by one or more point mutations, deletions, or inversions, but still encodes a functional MCT protein. In still another embodiment, one or more of the regulatory regions a promoter, repressor, or inducer) of an MCT gene in a microorganism has been altered by deletion, truncation, inversion, or point mutation) such that the expression of the MCT gene is modulated. One of ordinary skill in the art will appreciate that host cells containing more than one of the described MCT gene and protein modifications may be readily WO 01/00805 PCTIBOO/00926 -46produced using the methods of the invention, and are meant to be included in the present invention.

A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce express) an MCT protein. Accordingly, the invention further provides methods for producing MCT proteins using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding an MCT protein has been introduced, or into which genome has been introduced a gene encoding a wild-type or altered MCT protein) in a suitable medium until MCT protein is produced. In another embodiment, the method further comprises isolating MCT proteins from the medium or the host cell.

C. Isolated MCT Proteins Another aspect of the invention pertains to isolated MCT proteins, and biologically active portions thereof. An "isolated" or "purified" protein or biologically active portion thereof is substantially free of cellular material when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized. The language "substantially free of cellular material" includes preparations of MCT protein in which the protein is separated from cellular components of the cells in which it is naturally or recombinantly produced. In one embodiment, the language "substantially free of cellular material" includes preparations of MCT protein having less than about 30% (by dry weight) of non-MCT protein (also referred to herein as a "contaminating protein"), more preferably less than about 20% of non-MCT protein, still more preferably less than about 10% of non-MCT protein, and most preferably less than about 5% non-MCT protein. When the MCT protein or biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, culture medium represents less than about 20%, more preferably less than about 10%, and most preferably less than about 5% of the volume of the protein preparation. The language "substantially free of chemical precursors or other chemicals" includes preparations of MCT protein in which the protein is separated from chemical precursors or other chemicals which are involved in the synthesis of the protein. In one embodiment, the language "substantially free of chemical precursors or WO 01/00805 PCT/IB00/00926 -47other chemicals" includes preparations of MCT protein having less than about 30% (by dry weight) of chemical precursors or non-MCT chemicals, more preferably less than about 20% chemical precursors or non-MCT chemicals, still more preferably less than about 10% chemical precursors or non-MCT chemicals, and most preferably less than about 5% chemical precursors or non-MCT chemicals. In preferred embodiments, isolated proteins or biologically active portions thereof lack contaminating proteins from the same organism from which the MCT protein is derived. Typically, such proteins are produced by recombinant expression of, for example, a C. glutamicum MCT protein in a microorganism such as C. glulamicum.

An isolated MCT protein or a portion thereof of the invention can participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes, or has one or more of the activities set forth in Table 1. In preferred embodiments, the protein or portion thereof comprises an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) such that the protein or portion thereof maintains the ability participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes. The portion of the protein is preferably a biologically active portion as described herein. In another preferred embodiment, an MCT protein of the invention has an amino acid sequence set forth as an even-numbered SEQ ID NO: of the Sequence Listing.. In yet another preferred embodiment, the MCT protein has an amino acid sequence which is encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, to a nucleotide sequence of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing). In still another preferred embodiment, the MCT protein has an amino acid sequence which is encoded by a nucleotide sequence that is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%/o, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to one of the nucleic acid sequences of the invention, or a WO 01/00805 PCT/IBOO/00926 -48portion thereof. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. The preferred MCT proteins of the present invention also preferably possess at least one of the MCT activities described herein. For example, a preferred MCT protein of the present invention includes an amino acid sequence encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, to a nucleotide sequence of the invention, and which can participate in the metabolism of compounds necessary for the construction of cellular membranes in C. glutamicum, or in the transport of molecules across these membranes, or which has one or more of the activities set forth in Table 1.

In other embodiments, the MCT protein is substantially homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) and retains the functional activity of the protein of one of the amino acid sequences of the invention yet differs in amino acid sequence due to natural variation or mutagenesis, as described in detail in subsection I above. Accordingly, in another embodiment, the MCT protein is a protein which comprises an amino acid sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of the invention and which has at least one of the MCT activities described herein. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. In another embodiment, the invention pertains to a full length C. glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention.

WO 01/00805 PCT/IB00/00926 -49- Biologically active portions of an MCT protein include peptides comprising amino acid sequences derived from the amino acid sequence of an MCT protein, e.g., the an amino acid sequence of an even-numbered SEQ ID NO: of the Sequence Listing or the amino acid sequence of a protein homologous to an MCT protein, which include fewer amino acids than a full length MCT protein or the full length protein which is homologous to an MCT protein, and exhibit at least one activity of an MCT protein.

Typically, biologically active portions (peptides, peptides which are, for example, 10, 15, 20, 30, 35, 36, 37, 38, 39, 40, 50, 100 or more amino acids in length) comprise a domain or motif with at least one activity of an MCT protein. Moreover, other biologically active portions, in which other regions of the protein are deleted, can be prepared by recombinant techniques and evaluated for one or more of the activities described herein. Preferably, the biologically active portions of an MCT protein include one or more selected domains/motifs or portions thereof having biological activity.

MCT proteins are preferably produced by recombinant DNA techniques. For example, a nucleic acid molecule encoding the protein is cloned into an expression vector (as described above), the expression vector is introduced into a host cell (as described above) and the MCT protein is expressed in the host cell. The MCT protein can then be isolated from the cells by an appropriate purification scheme using standard protein purification techniques. Alternative to recombinant expression, an MCT protein, polypeptide,.or peptide can be synthesized chemically using standard peptide synthesis techniques. Moreover, native MCT protein can be isolated from cells endothelial cells), for example using an anti-MCT antibody, which can be produced by standard techniques utilizing an MCT protein or fragment thereof of this invention.

The invention also provides MCT chimeric or fusion proteins. As used herein, an MCT "chimeric protein" or "fusion protein" comprises an MCT polypeptide operatively linked to a non-MCT polypeptide. An "MCT polypeptide" refers to a polypeptide having an amino acid sequence corresponding to an MCT protein, whereas a "non-MCT polypeptide" refers to a polypeptide having an amino acid sequence corresponding to a protein which is not substantially homologous to the MCT protein, a protein which is different from the MCT protein and which is derived from the same or a different organism. Within the fusion protein, the term "operatively linked" is intended to indicate that the MCT polypeptide and the non-MCT polypeptide are fused WO 01/00805 PCT/IB00/00926 in-frame to each other. The non-MCT polypeptide can be fused to the N-terminus or Cterminus of the MCT polypeptide. For example, in one embodiment the fusion protein is a GST-MCT fusion protein in which the MCT sequences are fused to the C-terminus of the GST sequences. Such fusion proteins can facilitate the purification of recombinant MCT proteins. In another embodiment, the fusion protein is an MCT protein containing a heterologous signal sequence at its N-terminus. In certain host cells mammalian host cells), expression and/or secretion of an MCT protein can be increased through use of a heterologous signal sequence.

Preferably, an MCT chimeric or fusion protein of the invention is produced by standard recombinant DNA techniques. For example, DNA fragments coding for the different polypeptide sequences are ligated together in-frame in accordance with conventional techniques, for example by employing blunt-ended or stagger-ended termini for ligation, restriction enzyme digestion to provide for appropriate termini, filling-in of cohesive ends as appropriate, alkaline phosphatase treatment to avoid undesirable joining, and enzymatic ligation. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers.

Alternatively, PCR amplification of gene fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and reamplified to generate a chimeric gene sequence (see, for example, Current Protocols in Molecular Biology, eds. Ausubel et al. John Wiley Sons: 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety a GST polypeptide). An MCTencoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linked in-frame to the MCT protein.

Homologues of the MCT protein can be generated by mutagenesis, discrete point mutation or truncation of the MCT protein. As used herein, the term "homologue" refers to a variant form of the MCT protein which acts as an agonist or antagonist of the activity of the MCT protein. An agonist of the MCT protein can retain substantially the same, or a subset, of the biological activities of the MCT protein. An antagonist of the MCT protein can inhibit one or more of the activities of the naturally occurring form of the MCT protein, by, for example, competitively binding to a downstream or upstream member of the cell membrane component metabolic cascade which includes the MCT WO 01/00805 PCT/IB00/00926 -51 protein, or by binding to an MCT protein which mediates transport of compounds across such membranes, thereby preventing translocation from taking place.

In an alternative embodiment, homologues of the MCT protein can be identified by screening combinatorial libraries of mutants, truncation mutants, of the MCT protein for MCT protein agonist or antagonist activity. In one embodiment, a variegated library of MCT variants is generated by combinatorial mutagenesis at the nucleic acid level and is encoded by a variegated gene library. A variegated library of MCT variants can be produced by, for example, enzymatically ligating a mixture of synthetic oligonucleotides into gene sequences such that a degenerate set of potential MCT sequences is expressible as individual polypeptides, or alternatively, as a set of larger fusion proteins for phage display) containing the set of MCT sequences therein.

There are a variety of methods which can be used to produce libraries of potential MCT homologues from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA synthesizer, and the synthetic gene then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, in one mixture, of all of the sequences encoding the desired set of potential MCT sequences. Methods for synthesizing degenerate oligonucleotides are known in the art (see, Narang, S.A. (1983) Tetrahedron 39:3; Itakura et al. (1984) Annu. Rev. Biochem. 53:323; Itakura et al. (1984) Science 198:1056; Ike et al. (1983) Nucleic Acid Res. 11:477.

In addition, libraries of fragments of the MCT protein coding can be used to generate a variegated population of MCT fragments for screening and subsequent selection of homologues of an MCT protein. In one embodiment, a library of coding sequence fragments can be generated by treating a double stranded PCR fragment of an MCT coding sequence with a nuclease under conditions wherein nicking occurs only about once per molecule, denaturing the double stranded DNA, renaturing the DNA to form double stranded DNA which can include sense/antisense pairs from different nicked products, removing single stranded portions from reformed duplexes by treatment with SI nuclease, and ligating the resulting fragment library into an expression vector. By this method, an expression library can be derived which encodes N-terminal, C-terminal and internal fragments of various sizes of the MCT protein.

WO 01/00805 PCT/IB00/00926 -52- Several techniques are known in the art for screening gene products of combinatorial libraries made by point mutations or truncation, and for screening cDNA libraries for gene products having a selected property. Such techniques are adaptable for rapid screening of the gene libraries generated by the combinatorial mutagenesis of MCT homologues. The most widely used techniques, which are amenable to high through-put analysis, for screening large gene libraries typically include cloning the gene library into replicable expression vectors, transforming appropriate cells with the resulting library of vectors, and expressing the combinatorial genes under conditions in which detection of a desired activity facilitates isolation of the vector encoding the gene whose product was detected. Recursive ensemble mutagenesis (REM), a new technique which enhances the frequency of functional mutants in the libraries, can be used in combination with the screening assays to identify MCT homologues (Arkin and Yourvan (1992) PNAS 89:7811-7815; Delgrave et al. (1993) Protein Engineering 6(3):327-331).

In another embodiment, cell based assays can be exploited to analyze a variegated MCT library, using methods well known in the art.

D. Uses and Methods of the Invention The nucleic acid molecules, proteins, protein homologues, fusion proteins, primers, vectors, and host cells described herein can be used in one or more of the following methods: identification of C. glutamicum and related organisms; mapping of genomes of organisms related to C. glutamicum; identification and localization of C.

glutamicum sequences of interest; evolutionary studies; determination of MCT protein regions required for function; modulation of an MCT protein activity; modulation of the metabolism of one or more cell membrane components; modulation of the transmembrane transport of one or more compounds; and modulation of cellular production of a desired compound, such as a fine chemical.

The MCT nucleic acid molecules of the invention have a variety of uses. First, they may be used to identify an organism as being Corynebacterium glutamicum or a close relative thereof. Also, they may be used to identify the presence of C. glutamicum or a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of C glutamicum genes; by probing the WO 01/00805 PCT/IB00/00926 -53extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a C. glutamicum gene which is unique to this organism, one can ascertain whether this organism is present.

Although Corynebacterium glutamicum itself is nonpathogenic, it is related to pathogenic species, such as Corynebacterium diphtheriae. Corynebacterium diphtheriae is the causative agent of diphtheria, a rapidly developing, acute, febrile infection which involves both local and systemic pathology. In this disease, a local lesion develops in the upper respiratory tract and involves necrotic injury to epithelial cells; the bacilli secrete toxin which is disseminated through this lesion to distal susceptible tissues of the body. Degenerative changes brought about by the inhibition of protein synthesis in these tissues, which include heart, muscle, peripheral nerves, adrenals, kidneys, liver and spleen, result in the systemic pathology of the disease. Diphtheria continues to have high incidence in many parts of the world, including Africa, Asia, Eastern Europe and the independent states of the former Soviet Union. An ongoing epidemic of diphtheria in the latter two regions has resulted in at least 5,000 deaths since 1990.

In one embodiment, the invention provides a method of identifying the presence or activity of Cornyebacterium diphtheriae in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention the sequences set forth as odd-numbered or even-numbered SEQ ID NOs, respectively, in the Sequence Listing) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject. C. glutamicum and C. diphtheriae are related bacteria, and many of the nucleic acid and protein molecules in C. glulamicum are homologous to C diphtheriae nucleic acid and protein molecules, and can therefore be used to detect C. diphtheriae in a subject.

The nucleic acid and protein molecules of the invention may also serve as markers for specific regions of the genome. This has utility not only in the mapping of the genome, but also for functional studies of C. glutamicum proteins. For example, to identify the region of the genome to which a particular C. glutamicum DNA-binding protein binds, the C. glutamicum genome could be digested, and the fragments incubated with the DNA-binding protein. Those which bind the protein may be additionally probed with the nucleic acid molecules of the invention, preferably with readily detectable labels; binding of such a nucleic acid molecule to the genome fragment enables the WO 01/00805 PCTIBO00/00926 -54localization of the fragment to the genome map of C. glutamicum, and, when performed multiple times with different enzymes, facilitates a rapid determination of the nucleic acid sequence to which the protein binds. Further, the nucleic acid molecules of the invention may be sufficiently homologous to the sequences of related species such that these nucleic acid molecules may serve as markers for the construction of a genomic map in related bacteria, such as Brevibacterium laclofermentum.

The MCT nucleic acid molecules of the invention are also useful for evolutionary and protein structural studies. The metabolic and transport processes in which the molecules of the invention participate are utilized by a wide variety of prokaryotic and eukaryotic cells; by comparing the sequences of the nucleic acid molecules of the present invention to those encoding similar enzymes from other organisms, the evolutionary relatedness of the organisms can be assessed. Similarly, such a comparison permits an assessment of which regions of the sequence are conserved and which are not, which may aid in determining those regions of the protein which are essential for the functioning of the enzyme. This type of determination is of value for protein engineering studies and may give an indication of what the protein can tolerate in terms of mutagenesis without losing function.

Manipulation of the MCT nucleic acid molecules of the invention may result in the production of MCT proteins having functional differences from the wild-type MCT proteins. These proteins may be improved in efficiency or activity, may be present in greater numbers in the cell than is usual, or may be decreased in efficiency or activity.

The invention provides methods for screening molecules which modulate the activity of an MCT protein, either by interacting with the protein itself or a substrate or binding partner of the MCT protein, or by modulating the transcription or translation of an MCT nucleic acid molecule of the invention. In such methods, a microorganism expressing one or more MCT proteins of the invention is contacted with one or more test compounds, and the effect of each test compound on the activity or level of expression of the MCT protein is assessed.

There are a number of mechanisms by which the alteration of an MCT protein of the invention may directly affect the yield, production, and/or efficiency of production of a fine chemical from a C. glulamicum strain incorporating such an altered protein.

Recovery of fine chemical compounds from large-scale cultures of C. glutamicum is WO 01/00805 PCT/IBOO/00926 significantly improved if C. glutamicum secretes the desired compounds, since such compounds may be readily purified from the culture medium (as opposed to extracted from the mass of C. glutamicum cells). By either increasing the number or the activity of transporter molecules which export fine chemicals from the cell, it may be possible to increase the amount of the produced fine chemical which is present in the extracellular medium, thus permitting greater ease of harvesting and purification. Conversely, in order to efficiently overproduce one or more fine chemicals, increased amounts of the cofactors, precursor molecules, and intermediate compounds for the appropriate biosynthetic pathways are required. Therefore, by increasing the number and/or activity of transporter proteins involved in the import of nutrients, such as carbon sources sugars), nitrogen sources amino acids, ammonium salts), phosphate, and sulfur, it may be possible to improve the production of a fine chemical, due to the removal of any nutrient supply limitations on the biosynthetic process. Further, fatty acids and lipids are themselves desirable fine chemicals, so by optimizing the activity or increasing the number of one or more MCT proteins of the invention which participate in the biosynthesis of these compounds, or by impairing the activity of one or more MCT proteins which are involved in the degradation of these compounds, it may be possible to increase the yield, production, and/or efficiency of production of fatty acid and lipid molecules from C. glutamicum.

The engineering of one or more MCT genes of the invention may also result in MCT proteins having altered activities which indirectly impact the production of one or more desired fine chemicals from C .glutamicum. For example, the normal biochemical processes of metabolism result in the production of a variety of waste products hydrogen peroxide and other reactive oxygen species) which may actively interfere with these same metabolic processes (for example, peroxynitrite is known to nitrate tyrosine side chains, thereby inactivating some enzymes having tyrosine in the active site (Groves, J.T. (1999) Curr. Opin. Chem. Biol. 226-235). While these waste products are typically excreted, the C. glutamicum strains utilized for large-scale fermentative production are optimized for the overproduction of one or more fine chemicals, and thus may produce more waste products than is typical for a wild-type C.

glulamicum. By optimizing the activity of one or more MCT proteins of the invention which are involved in the export of waste molecules, it may be possible to improve the WO 01/00805 PCT/IBOO/00926 -56viability of the cell and to maintain efficient metabolic activity. Also, the presence of high intracellular levels of the desired fine chemical may actually be toxic to the cell, so by increasing the ability of the cell to secrete these compounds, one may improve the viability of the cell.

Further, the MCT proteins of the invention may be manipulated such that the relative amounts of various lipid and fatty acid molecules produced are altered. This may have a profound effect on the lipid composition of the membrane of the cell. Since each type of lipid has different physical properties, an alteration in the lipid composition of a membrane may significantly alter membrane fluidity. Changes in membrane fluidity can impact the transport of molecules across the membrane, which, as previously explicated, may modify the export of waste products or the produced fine chemical or the import of necessary nutrients. Such membrane fluidity changes may also profoundly affect the integrity of the cell; cells with relatively weaker membranes are more vulnerable in the large-scale fermentor environment to mechanical stresses which may damage or kill the cell. By manipulating MCT proteins involved in the production of fatty acids and lipids for membrane construction such that the resulting membrane has a membrane composition more amenable to the environmental conditions extant in the cultures utilized to produce fine chemicals, a greater proportion of the C. glutamicum cells should survive and multiply. Greater numbers of C. glutamicum cells in a culture should translate into greater yields, production, or efficiency of production of the fine chemical from the culture.

The aforementioned mutagenesis strategies for MCT proteins to result in increased yields of a fine chemical from C. glutamicum are not meant to be limiting; variations on these strategies will be readily apparent to one of ordinary skill in the art.

Using such strategies, and incorporating the mechanisms disclosed herein, the nucleic acid and protein molecules of the invention may be utilized to generate C. glutamicum or related strains of bacteria expressing mutated MCT nucleic acid and protein molecules such that the yield, production, and/or efficiency of production of a desired compound is improved. This desired compound may be any natural product of C.

glutamicum, which includes the final products of biosynthesis pathways and intermediates of naturally-occurring metabolic pathways, as well as molecules which do WO 01/00805 PCT/IB00/00926 -57not naturally occur in the metabolism of C. glutamicum, but which are produced by a C.

glutamicum strain of the invention.

This invention is further illustrated by the following examples which should not be construed as limiting. The contents of all references, patent applications, patents, published patent applications, Tables, and the sequence listing cited throughout this application are hereby incorporated by reference.

TABLE 1: GENES IN THE APPLICATION Nucleic Acid SEQ ID NO Amino Acid Identification Code SEQ ID NO 2 RXN03097 4 RXA02099 6 RXA00104 Contig W0062 GROO630 GROO014 NT Start NT Stop Function 3 6198 15895 557 6470 16650 AMMONIUM TRANSPORT SYSTEM AMMONIUM TRANSPORT SYSTEM CYSO PROTEIN, ammonium transport protein Polyketide Synthesis Nucleic Acid SEQ ID NO 7 9 11 13 17 19 21 Amino Acid Identification Code SEQ ID NO 8 RXA01420 10 RXN02581 12 F RXA02581 14 RXA02582 16 RXA01 138 18 RXA01980 20 RXN01007 22 RXN00784 Conttg GR00416 WV0098 GR00741 GR00741 GR00318 GR00573 WV0021 WV0103 NT Start NT Stop Function 775 30482 1890 1656 1470 2572 27531 17 28623 1527 6719 2072 838 866 28265 4*-MYCAROSYL ISOVALERYL-COA TRANSFERASE (EC POLYKETIDE SYNTHASE POLYKETIDE SYNTHASE PROBABLE POLYKETIDE SYNTHASE CY338.20 ACTINORHODIN POLYKETIDE DIMERASE (EC POLYKETIDE CYCLASE

FRNA

FRNE

Fatty acid and lipid synthesis Nucleic Acid Amino Acid Identification Code SEQ ID NO SEQ IDNO 23 24 RXA02335 26 RXA02173 Contigl NT Start NT Stop Function RXA01764 RXN02487 F RXA02487 F RXA02490 RXA01 467 RXA00796 RXA01 897 RXN02809 F RXA02809 RXN001 13 F RXA00l 13 GR00672 550 2322 BIOTIN CARBOXYLASE (EC 6.3.4.14) GR00641 7473 8924 ACETYL-COENZYME A CARBOXYLASE CARBOXYL TRANSFERASE SUBUNIT BETA (EC 6.4.1.2) GROO500 2178 3110 3-OXOACYL-[ACYL-CARRIER PROTEIN] REDUCTASE (EC 1.1.1 .100) W0007 6367 4664 LONG-CHAIN-FATTY-ACID-COA LIGASE (EC 6.2.1.3) GR00718 4937 4650 LONG-CH(AIN-FATTY-ACID-COA LIGASE (EC 6.2.1.3) GR00720 817 5 LONG-CHAIN-FATTY-ACID-COA LIGASE (EC 6.2.1.3) GR00422 920 1210 ACYL CARRIER PROTEIN GROO21 2 202 5 Acyl carrier protein phosphodiesterase GR00544 617 1159 Acyl carrier protein phosphodiesterase W0342 380 6 Acyl carrier protein phosphodiesterase GR00790 277 5 Acyl carrier protein phosphodiesterase W0129 103 5724 FATTY ACID SYNTHASE (EC 2.3.1.85) [INCLUDES: EC 2.3.1.38; EC 2.3.1.39; EC 2.3.1.41; GROOO1 7 2 3295 FATTY-ACID SYNTHASE (EC 2.3.1.85) Nucleic Acid SEQ ID NO 49 Am~ino Acid SEQ ID NO 50 Identification Code RXN03 111 F RXA001 58 F RXA00572 RXA02582 RXA02691 RXA00880 RXA0 1060 RXN01 722 F RXA01 722 RXA01644 RXA02029 RXA01 801 RXN0251 2 F RXA02512 RXA00899 RXN0O819 F RXA00819 F RXA01766 RXN01 762 F RXA01 762 RXA00681 RXA00802 RXA021 33 RXN01114 F RXAO1 11 4 RXAO1 894 RXA02599 RXN02638 F RXA02638 RXA00856 Table 1 (continued) Contig. NT Start NT Stog, Function W0084 6040 5 FATTY ACID SYNTHASE (EC 2.3.1.85) [INCLUDES: EC 2.3.1.38, EC 2.3.1.39; EC 2.3.1.41; EC 1.1.1.100; EC 4.2.1.61; EC 1.3.1.10; EC 3.1.2.14] GR00024 2088 4 FATTY ACID SYNTHASE (EC 2.3.1.85) GR00155 2 3832 FATTY ACID SYNTHASE (EC 2.3.1.85) GR00741 1890 6719 PROBABLE POLYKETIDE SYNTHASE CY338.20 GR00754 15347 14541 FATTY ACYL RESPONSIVE REGULATOR GR00242 6213 8057 LONG-CHAIN-FATTY-ACID-COA LIGASE (EC 6.2.1.3) GR00296 9566 10489 OMEGA-3 FATTY ACID DESATURASE (EC 1. 14.99.-) W0036 2938 1214 MEDIUM-CH(AIN-FATTY-ACID-COA LIGASE (EC GR00488 5746 4022 MEDIUM-CHIAIN-FATTY-ACID-COA LIGASE (EC GR00456 9854 8577 CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHASE (EC 2.1.1.79) GR00618 356 1669 CYCLOPROPANE-FATTY-ACYL-PHOSPHOLIPID SYNTHIASE (EC 2.1 .1.79) GR00509 3396 2380 ENOYL-COA HYDRATASE (EC 4.2.1.17) W0171 16147 15185 LIPID A BIOSYNTHESIS LAUROYL ACYLTRANSFERASE (EC GR00721 3303 4259 LIPID A BIOSYNTHESIS LAUROYL ACYLTRANSFERASE (EC GR00245 1599 2864 CARDIOLIPIN SYNTHETASE (EC W0054 18127 19455 ACYL-COA DEHYDROGENASE (EC 1.3.99.-) GROO221 18 1007 ACYL-COA DEHYDROGENASE (EC 1.3.99.-) GROO500 4081 4371 ACYL-COA DEHYDROGENASE (EC 1.3.99.-) W0054 15318 13783 LONG-CHAIN-FATTY-ACID-COA LIGASE (EC 6.2.1.3) GR00500 1272 10 LONG-CHIAIN-FATTY-ACID-COA LIGASE (EC 6.2.1.3) GR00179 3405 2662 3-OXOACYL-fACYL-CARRIER PROTEIN) REDUCTASE (EC 1.1.1.100) GR00214 3803 4516 3-OXOACYL-[ACYL-CARRIER PROTEIN] REDUCTASE (EC 1.1.1.100) GR00639 3 308 3-OXOACYL-[ACYL-CARRIER PROTEIN] REDUCTASE (EC 1.1.1 .100) W0182 9118 10341 3-KETOACYL-COA THIOLASE (EC 2.3.1.16) GR00308 2 793 3-KETOACYL-COA THIOLASE (EC 2.3.1.16) GR00542 1622 2476 PHOSPHATIDATE CYTIDYLYLTRANSFERASE (EC 2.7.7.41) GR00742 3179 3655 PHOSPHiATIDYLGLYCEROPHOSPHiATASE B (EC 3.1.3.27) W0098 54531 53656 1 -ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (EC 2.3.1.51) GR00749 8 511 1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (EC 2.3.1.51) GR00232 720 1256 CDP-DIACYLGLYCEROL--GLYCEROL-3-PHOSPHATE 3- PHOSPHATIDYLTRANSFERASE (EC 2.7.8.5) GR00721 2621 3277 CDP-DIACYLGLYCEROL-GLYCEROL-3-PHOSPHATE 3- PHOSPHATIDYLTRANSFERASE (EC 2.7.8.5) W0102 32818 33372 KETOACYL REDUCTASE HETN (EC GR00827 106 411 KETOACYL REDUCTASE HETN (EC GR00740 2438 3541 PUTATIVE ACYLTRANSFERASE GR00639 18858 19658 1 -ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE (EC 2.3.1.51) GROO160 1869 2249 POLY(3-HYDROXYALKANOATE) POLYMERASE (EC GR00698 1688 2683 POLY-BETA-HYDROXYBUTYRATE POLYMERAS E (EC 2.3. W0083 16568 17929 HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6) GRO01 71 1027 5 HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6) GR00214 3138 3770 HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6) GR00221 1469 2311 HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6) W0143 12056 13462 HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6) GR00517 1666 260 HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6) GR00525 5561 5010 HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6) 109 110 RXA02511 ill 112 RXN02836 113 114 F RXA02836 115 116 RXA02578 117 118 RXA02150 119 120 RXA00607 121 122 RXA02397 123 124 RXN031 10 125 126 F RXA00660 127 128 RXA00801 129 130 RXA00821 131 132 RXN02966 133 134 F RXA01833 135136 RXA01853 Table I (continued) NT Start NT Stop Function Nucleic Acid Amino Acid SEQ ID NO SEQID0NO 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 identification Code Contig.

RXN02424 F RXA02424 RXN00419 F RXA00419 F RXA00421 RXN02923 RXN02922 RXN03065 RXN03132 RXN031 57 RXN00934 RXN00792 RXN00931 F RXA00931 RXN01421 RXN02342 RXN00563 RXN02168 RXN01 090 RXN02062 RXN02148 RXN02595 RXS00148 RXS00149 RXS021 06 RXS01 746 RXS01 747 RXCO1748 RXCO0354 RXCO1 749 W01 16 10570 11169 HYDROXYACYLGILUTATHIONE HYDROLASE (EC 3.1.2.6) GR00706 808 428 HYDROXYACYLGLUTATHIONE HYDROLASE (EC 3.1.2.6) W0112 1024 266 ACETOACETYL-COA REDUCTASE (EC 1.1.1.36) GR00095 3 464 ACETOACETYL-COA REDUCTASE (EC 1.1.1.36) GR00096 565 723 ACETOACETYL-COA REDUCTASE (EC 1.1.1.36) W0088 3301 2564 ACETOACETYL-COA REDUCTASE (EC 1.1.1.36) W03211 11407 10328 ACYL-COA DEHYDROGENASE, SHORT-CHAIN SPECIFIC (EC 1.3.99.2) W0038 6237 6629 HOLO-fACYL-CARRIER PROTEIN] SYNTHASE (EC 2.7.8.7) W0127 39053 39472 POLY-BETA-HYDROXYBUTYRATE POLYMERASE (EC W0188 1607 1170 LIPOPOLYSACCHARIDE CORE BIOSYNTHESIS PROTEIN KDTB W0171 15181 14099 (AE000805) LPS biosynthesis RfbU related protein (Methanobacterium thermoautotrophicum] W0321 10328 9132 ACYL-COA DEHYDROGENASE, SHORT-CHAIN SPECIFIC (EC 1.3.99.2) W0171 13011 12166 ACYL-COA THIOESTERASE 11 (EC GR00253 4959 4114 thioesterase 11 W0122 16024 15638 ACYLTRANSFERASE (EC W0078 3460 4266 BIOTIN-[ACETYL-COA-CARBOXYLASE] SYNTHETASE (EC 6.3.4.15) W0038 1 2739 FATTY ACID SYNTHASE (EC 2.3.1.85) [INCLUDES: EC 2.3.1.38: EC 2.3.1.39 EC 2.3.1.41; EC 1.1.1.100; EC 4.2.1.61; EC 1.3.1.10; EC 3.1.2.141 W0100 2894 81 FATTY ACID SYNTHASE (EC 2. 3.1.85) [INCLUDES: EC 2.3.1.38; EC 2.3.1.39: EC 2.3.1.4 1: EC 1. 1.1. 100: EC 4.2.1.61; EC 1.3. 1. 10; EC 3.1.2.141 W0155 6483 5686 KETOACYL REDUCTASE HETN (EC W0222 3159 1990 Lipopotysaccha ride N-acetylgluoosaminyltransfe-rase W0300 16561 17703 Lipopotysaccha ride N-acetylglucosaminyltransferase W0098 11098 9935 Lipopolysacchadde N-acetylglucosaminyltransferase W0167 9849 12059 METHYLMALONYL-OA MUTASE ALPHA-SUBUNIT (EC 5.4.99.2) W0167 7995 9842 METHYLMALONYL-COA, MUTASE BETA-SUBUNIT (EC 5.4.99.2) W0123 22649 21594 LIPOATE-PROTEIN LIGASE A (EC W0185 934 1686 LIPOATE-PROTEIN LIGASE B (EC W01 85 1826 2869 LIPOIC ACID SYNTHETASE W0185 30301 3780 protein involved in lipid metabolism W01 35 33604 32792 Cytosolic Protein involved in lipid metabolism W0185 3953 5569 Membrane Spanning Protein involved in lipid metabolism Fatty acid degradation Nucleic Acid Amino Acid Identification Code Contig NT Start NT Stop Function SEQ ID NO SEQ ID NO 197 198 RXA02268 GR00655 2182 3081 LIPASE (EC 3.1.1.3) 199 200 RXA02269 GR00655 3094 4065 LIPASE (EC 3.1.1.3) 201 202 RXA01614 GR00449 8219 7197 LYSOPHOSPHOLIPASE L2 (EC 3.1.1.5) 203 204 RXA01983 GR00573 3559 3053 LIPASE (EC 3.1.1.3) 205 206 RXN02947 W0078 1319 6 PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3) 207 208 F RXA02320 GR00667 593 6 PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3) 209 210 F RXA02851 GR00851 524 6 PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3) 211 212 RXN02321 W0078 3291 1663 PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3) Nucleic Acid SEQ ID NO 213 215 217 219 221 Amino Acid SEQ ID NO 214 .216 218 220 222 223 224 225 226 Identification Code F RXA02321 F RXA02343 F RXA02850 RXA02583 RXA00870 RXA01260 RXA01261 RXA01 136 RXN00559 F RXA00559 RXA01580 RXA02677 RXS01 166 Table I (continued) Contig. NT Start NT Stor Function GR00667 1380 937 PROPIONYL-OA CARBOXYLASE BETA CHAIN (EC 6.4.1.3) GR00675 1403 1816 PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3) GROO850 2 493 PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3) GR00741 6743 8290 PROPIONYL-COA CARBOXYLASE BETA CHAIN (EC 6.4.1.3) GR00239 809 2320 METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE (ACYLATING) (EC 1.2.1.27) 2-Methyl-3-oxopropanoate:NAD+ oxidoreductase (CoA-propanoylating) GR00367 2381 1200 LIPOAMIDE DEHYDROGENASE COMPONENT (E3) OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX (EC 1.8.1.4) GR00367 2607 2437 LIPOAMIDE DEHYDROGENASE COMPONENT (E3) OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX (EC 1.8.1.4) GR00318 685 1116 ISOVALERYL-COA DEHYDROGENASE (EC 1.3.99.10) W0103 7568 6552 PROTEIN VDLD GR00149 218 6 PROTEIN VOLO GR00440 707 6 Glycerophosphoryl diester phosphodiesterase GR00754 3119 3877 GLYCEROPHOSPHORYL DIESTER PHOSPHODIESTERASE (EC 3.1.4.46) W0117 18142 16838 EXTRACELLULAR LIPASE PRECURSOR (EC 3.1.1.3) Terpenoid biosynthesis Nucleic Acid SEQ ID NO 239 241 243 245 247 249 251 Amino Acid SEQ ID NO 240 242 244 246 248 250 252 Identification Code RXA00875 RXA01 292 RXAO1 293 RXA02310 RXA0271 8 RXA01067 RXA01269 Contiq.

NT Start NT Stop Function 253 254 RXA01 205 RXA01 576 RXN02309 F RXA02309 RXN00477 F RXA00477 RXA00478 RXA01291 RXAOD480 RXS01 879 RXS02023 RXS00948 RXS02228 RXCO1971 RXC02697 GR00241 2423 1857 ISOPENTENYL-DI PHOSPHATE DELTA-ISOMERASE (EC 5.3.3.2) GR00373 1204 2388 PHYTOENE DEHYDROGENASE (EC GR00373 2370 2696 PHYTOENE DEHYDROGENASE (EC GR00665 1132 2394 GERANYLGERANYL HYDROGENASE GR00758 18539 19585 GERANYLGERANYL PYROPHOSPHATE SYNTHASE (EC 2.5.1.1) GR00298 1453 2181 undecaprenyl-diphosphate synthase (EC 2.5.1.31) GR00367 20334 19894 UNDECAPRENYL-PHOSPHATE GALACTOSEPHOSPHOTRANSFERASE (EC 2.7.8.6) GR00346 3 533 PUTATIVE UNDECAPRENYL-PHOSPHATE ALPHA-N- ACETYLGLUCOSAMINYLTRANSFERASE (EC GR00438 8053 8811 DOLICHYL-PHOSPHATE BETA-GLUCOSYLTRANSFERASE (EC 2.4.1.117) W0025 28493 29542 OCTAPRENYL-DIPHOSPHATE SYNTHASE (EC 2.5. GR00665 978 4 OCTAPRENYL-DIPHOSPHATE SYNTHASE (EC W0086 38905 37262 PHYTOENE DEHYDROGENASE (EC GRO01 19 13187 11544 PHYTOENE DEHYDROGENASE (EC GROWl19 14020 13190 PHYTOENE SYNTHASE (EC GR00373 345 1277 PHYTOENE SYNTHASE (EC GROW119 17444 16329 FARNESYL DIPHOSPHATE SYNTHASE (EC 2.5.1.11) (EC 2.5.1.10) W01 05 1505 573 isopentenyl-phosphate kinase (EC W0160 3234 4001 P450 cytochrome,isopentenyltransf, ferridox W0107 4266 5384 12-oxophytodienoate reductase (EC 1.3.1.42) W0068 1876 2778 TRNA DELTA(2)-ISOPENTENYLPYROPHOSPHATE TRANSFERASE (EC 2.5.1.8) W0105 4545 3715 Metal-Dependent Hydrolase involved in metabolism of terpenoids W0017 31257 32783 membrane protein involved in metabolism of terpenoids Table I (continued) ABC-Transporter 0 Nucleic Add Amino Acid Identification Code Contig. NT Start NT Stop Function SEQ ID NO SEQ ID NO 283 284 RXN01946 W0228 2 1276 Hypothetical ABC Transporter ATP-Binding Protein 285 286 F RXA01946 GR00559 1849 575 (AL021184) ABC transporter ATP binding protein [Mycobacterium tuberculosis] 287 288 RXN00164 W0232 1782 94 Hypothetical ABC Transporter ATP-Binding Protein 289 290 F RXA00164 GR00025 1782 94 P, G, R ATPase subunits of ABC transporters 291 292 RXN00243 W0057 28915 27899 P, G. R ATPase subunits of ABC transporters 293 294 F RXA00243 GR00037 930 4 P, G, R ATPase subunits of ABC transporters 295 296 RXA00259 GR00039 8469 6268 P, G, R ATPase subunits of ABC transporters 297 298 RXN00410 W0086 51988 51323 GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ 299 300 F RXA00410 GR00092 829 164 P, G, R ATPase subunits of ABC transporters 301 302 RXN00456 W0076 6780 8156 P, G. R ATPase subunits of ABC transporters 303 304 F RXA00456 GR00114 316 5 G, R ATPase subunits of ABC transporters 305 306 F RXA00459 GR00115 1231 245 P. G. R ATPase subunits of ABC transporters 307 308 RXN01604 W0137 8117 7470 P, G, R ATPase subunits of ABC transporters 309 310 F RXA01604 GR00448 2 607 P, G, R ATPase subunits of ABC transporters 311 312 RXN02547 W0057 27726 25588 P, G, R ATPase subunits of ABC transporters 313 314 F RXA02547 GR00726 22055 19932 P, G, R ATPase subunits of ABC transporters 315 316 RXN02571 W0101 12331 13359 MALTOSE/MALTODEXTRIN TRANSPORT ATP-BINDING PROTEIN MALK 317 318 F RXA02571 GR00736 1469 2497 P. G, R ATPase subunits of ABC transporters 319 320 RXN02074 W0318 12775 11153 TRANSPORT ATP-BINDING PROTEIN CYDD 321 322 F RXA02074 GR00628 5798 4176 P, G. R ATPase subunits of ABC transporters 323 324 RXA02095 GR00629 14071 15474 P, G, R ATPase subunits of ABC transporters 325 326 RXA02225 GR00652 3156 2275 P, G. R ATPase subunits of ABC transporters 327 328 RXA02253 GR00654 20480 21406 P, G, R ATPase subunits of ABC transporters 329 330 RXN01881 W0105 529 95 Hypothetical ABC Transporter ATP-Binding Protein 331 332 F RXA01881 GR00537 3092 3532 ATPase components of ABC transporters with duplicated ATPase domains 333 334 RXA00526 GR00136 1353 664 Hypothetical ABC Transporter ATP-Binding Protein 335 336 RXN00733 W0132 1647 2531 Hypothetical ABC Transporter ATP-Binding Protein 337 338 F RXA00733 GR00197 411 4 Hypothetical ABC Transporter ATP-Binding Protein 339 340 RXA00735 GR00198 849 181 Hypothetical ABC Transporter ATP-Binding Protein 341 342 RXA00878 GR00242 3733 1871 Hypothetical ABC Transporter ATP-Binding Protein 343 344 RXN01191 W0169 10478 12067 Hypothetical ABC Transporter ATP-Binding Protein 345 346 F RXA01191 GR00341 1571 165 Hypothetical ABC Transporter ATP-Binding Protein 347 348 RXN01212 W0169 3284 4207 Hypothetical ABC Transporter ATP-Binding Protein 349 350 F RXA01212 GR00350 1 813 Hypothetical ABC Transporter ATP-Binding Protein 351 352 RXA02749 GR00764 4153 5028 Hypothetical ABC Transporter ATP-Binding Protein 353 354 RXA02224 GR00652 2271 475 Hypothetical ABC Transporter ATP-Binding Protein 355 356 RXN01602 W0229 1109 2638 Hypothetical ABC Transporter ATP-Binding Protein 357 358 RXN02515 W0087 962 1717 Hypothetical ABC Transporter ATP-Binding Protein 359 360 RXN00525 W0079 26304 27566 Hypothetical ABC Transporter Permease Protein 361 362 RXN02096 W0126 20444 22135 Hypothetical ABC Transporter Permease Protein 363 364 RXN00412 W0086 53923 52844 Hypothetical Amino Acid ABC Transporter ATP-Binding Protein 365 366 RXN00411 W0086 52844 52170 Hypothetical Amino Acid ABC Transporter Permease Protein 367 368 RXN02614 W0313 5964 5236 TAURINE TRANSPORT ATP-BINDING PROTEIN TAUB 369 370 RXN02613 W0313 5223 4267 TAURINE-BINDING PERIPLASMIC PROTEIN PRECURSOR Nucleic Acid SEQ ID NO 371 373 375 377 379 381 383 385 387 389 391 393 395 Amino Acid SEQ ID NO 372 374 376 378 380 382 384 386 388 390 392 394 396 Identification Code RXN00368 F RXA00368 F RXA00370 RXN01 285 RXN 00523 RXN01 142 RXN01 141 RXN01 002 RXNO1 000 RXN01 732 RXN03080 RXN03081 RXN03082 922!t.

W0226 GR00076 GROO077 W0215 W01 94 W0077 W0077 W0V1 06 W01 06 W0106 WV0045 W0045 VV0045 Table I (continued) NT Start NT Stop Function 2300 726 SPERMIDINEIPUTRESCINE TRANSPORT ATP-BINDING PROTEIN POTA 1 579 SPERMIDINEIPLJTRESCINE TRANSPORT ATP-BINDING PROTEIN POTA 6 803 SPERMIDINE/PUTRESCINE TRANSPORT ATP-BINDING PROTEIN POTA 1780 1055 FERRIC ENTEROBACTIN TRANSPORT ATP-BINDING PROTEIN FEPC 1363 338 FERRIC ENTEROBACTIN TRANSPORT PROTEIN FEPG 5805 6302 NITRATE TRANSPORT ATP-BINDING PROTEIN NRTD 4644 5468 NITRATE TRANSPORT PROTEIN NRTA 8858 8055 PHOSPHONATES TRANSPORT ATP-BINDING PROTEIN PHNC 7252 6407 PHOSPHONATES TRANSPORT SYSTEM PERMEASE PROTEIN PHNE 9944 8895 PHOSPHONATES-BINDING PERIPLASMIC PROTEIN PRECURSOR '1670 2449 FERRIC ENTEROBACTIN TRANSPORT ATP-BINDING PROTEIN FEPC 2476 2934 FERRIENTEROBACTIN-BINDING PERIPLASMIC PROTEIN PRECURSOR 3131 3451 FERRIENTEROBACTIN-BINDING PERIPLASMIC PROTEIN PRECURSOR Other transporters Nucleic Acid SEQ ID NO 397 399 401 403 405 407 409 411 413 415 417 419 421 423 425 427.

429 431 433 435 437 439 441 "43 445 447 "49 An o Acid SEQ ID NO 398 400 402 404 406 408 410 412 414 416 418 420 422 424 426 428 430 432 434 436 438 440 442 444 446 448 450 Identification Code RXA02261 RXA02020 RXA00281 RXN00570 F RXA00570 RXN00571 F RXA00571 RXA00962 RXA028I11 RXA021 15 RXN00590 F RXA00590 F RXA01538 RXA01 727 RXA00623 RXA01584 RXA00852 RXA00690 RXA00827 RXA0O851 RXS03220 F RXA02762 RXN00092 F RXA00092 RXN03060 F RXA02618 F RXA02900 GR00654 GR00613 GR00043 W0147 GR00153 W01 73 GROO1 54 GR00268 GR00792 GR00635 W01 78 GR00157 GR00427 GR00489 GR00163 GROD441 GR00231 GROG 181 GR00223 GR00231 GR00768 W01 29 GROO014 W0030 GR00745 GRIO040 NT Start NT Stop Function 30936 1015 4721 855 1 1298 2 2 177 2 5043 178 5040 1471 6525 55 3137 1213 1319 2448 346 27509 1 6227 1914 2979 32291 5 5404 4 498 42 1186 667 560 1198 6230 564 5429 194 7862 597 2448 68 567 1840 630 26844 204 5376 2351 2128 AMMONIUM TRANSPORT SYSTEM AROMA11C AMINO ACID TRANSPORT PROTEIN AROP BACrrRACIN TRANSPORT ATP-BINDING PROTEIN BCRA BENZOATE MEMBRANE TRANSPORT PROTEIN BENZOATE MEMBRANE TRANSPORT PROTEIN BENZOATE MEMBRANE TRANSPORT PROTEIN BENZOATE MEMBRANE TRANSPORT PROTEIN BENZOATE MEMBRANE TRANSPORT PROTEIN BENZOATE MEMBRANE TRANSPORT PROTEIN BENZOATE MEMBRANE TRANSPORT PROTEIN BRANCHED CHAIN AMINO ACID TRANSPORT SYSTEM 11 CARRIER PROTEIN BRANCHED CHAIN AMINO ACID TRANSPORT SYSTEM 11 CARRIER PROTEIN BRANCHED CHAIN AMINO ACID TRANSPORT SYSTEM 11 CARRIER PROTEIN BRANCHED-CHAIN AMINO ACID TRANSPORT SYSTEM CARRIER PROTEIN C4-DICARBOXYLATE TRANSPORT PROTEIN CHROMATE TRANSPORT PROTEIN COBALT TRANSPORT ATP-BINDING PROTEIN CBIO COBALT TRANSPORT PROTEIN CBIO COBALT TRANSPORT PROTEIN CBIQ COBALT TRANSPORT PROTEIN CBIQ D-XYLOSE-PROTON SYMPORT D-XYLOSE PROTON-SYMPORTER GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ GLUTAMINE TRANSPORT ATP-BINDING PROTEIN GLNQ Table 1 (continued) Nucleic Acid Amino Acid Identification Code Cti. NT Start NT Stop Function SEQI ID NO SEQ ID NO 0 451 452 RXS03212 GLYCINE BETAINE TRANSPORTER BETIP 453 454 F RXAO1 591 GR00446 3 947 GLYCINE BETAINE TRANSPORTER BETP 455 456 RXN00201 W0096 197 6 HIGH AFFINITY RIBOSE TRANSPORT PROTEIN RBSD 457 458 F RXA00201 GR00032 191 6 HIGH AFFINITY RIBOSE TRANSPORT PROTEIN RBSD0 459 460 RXA01 221 GR00354 2108 2833 HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN BRAG 461 462 RXA01222 GR00354 2844 3542 HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT ATP-BINDING PROTEIN LIVF 463 464 RXA01219 GR00354 151 1032 HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT PERMEASE PROTEIN LIVH 465 466 RXA01220 GR00354 1032 2108 HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT PERMEASE PROTEIN LIVM 467 468 RXA00091 GROO013 7762 8514 IRON(III) DIC~TRATE TRANSPORT ATP-BINDING PROTEIN FECE 469 470 RXA00228 GRO0032 29232 28642 IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE 471 472 RXA00346 GR00064 1054 1743 IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE 473 474 RXA00524 GROW135 779 11111 IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE 475 476 RXAO1 823 GR00516 591 1367 IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE 477 478 RXA02767 GR00770 1032 1814 IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE 479 480 RXA02792 GR00777 8581 7829 IRON(III) DICITRATE TRANSPORT ATP-BINDING PROTEIN FECE 481 482 RXN02929 WO090 36837 37874 IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD 483 484 F RXAO1 235 GR00358 1165 194 IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD 485 486 RXN02794 W0134 10625 9552 IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD 487 488 F RXAO1 419 GR00415 888 1151 IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD 489 490 F RXA02794 GR00777 10172 9552 IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD 491 492 RXN03079 W0045 644 1660 IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECO 493 494 F RXA02865 GRI 0007 3832 2816 IRON(III) DICITRATE TRANSPORT SYSTEM PERMEASE PROTEIN FECD 495 496 RXAOO181 GR00028 3954 2363 PROLINE TRANSPORT SYSTEM 497 498 RXA00591 GR00158 229 1581 PROLINEfBETAINE TRANSPORTER 499 500 RXA01629 GR00453 3476 1965 PROLINE/BETAINE TRANSPORTER 501 502 RXA02030 GR00618 3072 1687 PROLINEIBETAINE TRANSPORTER 503 504 RXA00186 GR00028 12242 12988 SHORT-CHAIN FATTY ACIDS TRANSPORTER 505 506 RXA00187 GR00028 13097 13447 SHORT-CHAIN FATTY ACIDS TRANSPORTER 507 508 RXA01 667 GR00464 703 1908 SODIUM/GLUTAMATE SYMPORT CARRIER PROTEIN 509 510 RXA02171 GROO641 6571 4919 SODIUMIPROLINE SYMPORTER 511 512 RXA00902 GR00245 4643 5875 SODIUM-DEPENDENT PHOSPHATE TRANSPORT PROTEIN 513 514 RXA00941 GR00257 1999 683. sodium-dependent phosphate transport protein 0 515 516 RXNO0449 W01 12 30992 32572 Sodium-Dicartboxylate Symport Proteinn 517 518 F RXA00449 GROO109 2040 1036 Sodium-Dicarbaxylate Symport Protein 519 520 F RXA01755 GR00498 352 5 Sodium-Dicarboxylate Symport Protein 5211 522 RXA00269 GROOO41 1826 1038 SPERMIDINE/PUTRESCINE TRANSPORT ATP-BINDING PROTEIN POTA 523 524 RXA00369 GR00076 583 1299 SPERMIDINE/PUTRESCINE TRANSPORT ATP-BINDING PROTEIN POTA 525 526 RXA02073 GR00628 4176 2647 TRANSPORT ATP-BINDING PROTEIN CYDC 527 528 RXA01399 GR00409 1 1119 TRANSPORT ATP-BINDING PROTEIN CYDD 529 530 RXA01 339 GR00389 8408 7164 TYROSINE-SPECIFIC TRANSPORT PROTEIN 531 532 RXA02527 GR00725 5519 6847 2-OXOGLUTARATEMALATE TRANSILOCATOR PRECURSOR 533 534 RXN00298 W0176 40228 42072 HIGH-AFFINITY CHOLINE TRANSPORT PROTEIN 535 536 F RXA00298 GR00048 4459 6303 Ectoine/Proline/Glycine betaine carrier ectP Table 1 (continued) NT Start NT Stop Function Nucleic Acid Anmino Acid Identification Code Contig.

SEQ ID NO SEQ ID NO 537 538 RXA00596 GRO01lt 539 540 RXA02364 (5R0061 ~9 335.

36 841 787 potassium efflux system protein phaE 215 C4-DICARBOXYLATE-BINDING PERIPLASMIC PROTEIN PRECURSOR, transport RXN0141 1 RXN00960 RXN02447 RXN02395 RXN02348 RXN00297 RXN03103 RXN02993 RXN00349 RXN03095 RXN03160 RXN02955 RXN031 09 RXN02979 RXN02987 RXN03084 RXN03183 RXNO1 139 RXN00378 RXN01 338 RXN00980 RXN00099 RXN02662 RXN02442 RXN02443 RXN00842 F RXA00842 RXND0832 RXNDD466 RXN01936 RXNO1 995 RXN00661 W0050 26015 W0075 1139 W0107 14297 W0176 16747 W0078 6027 W0176 38630 W0070 845 W0071 736 W0135 35187 WOS? 4056 W0189 5150 W0176 8666 W0082 659 W0149 2150 W0234 527 W0048 900 W0372 1 W0077 2776 W0223 8027 W0032 2 W0149 2635 W0129 18876 W0315 1461 W0217 5970 W0217 6818 W0138 8686 GR00228 3208 W0180 3133 W0086 63271 W0127 40116 W01 82 2139 W0142 9718 26779 105 13203 14858 7910 39541 1087 65 36653 4424 5617 9187 6 2383 294 1817 417 1823 5418 1903 4428 17704 1724 6818 7771 7487 2009 4182 64266 41387 3476 9029 protein SHIKIMATE TRANSPORTER PROTON/SODIUM-GLUTAMATE SYM PORT PROTEIN GALACTOSE-PROTON SYMPORT GLYCINE BETAINE TRANSPORTER BETP KUP SYSTEM POTASSIUM UPTAKE PROTEIN Hypothetical Malonate Transporter GLUTAMATE-BINDING PROTEIN PRECURSOR GLUTAMINE-BINDING PROTEIN Hypothetical Trehalose Transport Protein CADMIUM EFFLUX SYSTEM ACCESSORY PROTEIN HOMOLOG CHROMATE TRANSPORT PROTEIN DICARBOXYLATE TRANSPORTER HEMIN TRANSPORT SYSTEM PERMEASE PROTEIN HMUU MERCURIC TRANSPORT PROTEIN PERIPLASMIC COMPONENT PRECURSOR MERCURIC TRANSPORT PROTEIN PERIPLASMIC COMPONENT PRECURSOR IRON(III) DICITRATE-BINDING PERIPLASMIC PROTEIN PRECURSOR TREHALOSE/MALTOSE BINDING PROTEIN CATION EFFLUX SYSTEM PROTEIN CZCD Cation transport ATPases CATION-TRANSPORTING ATPASE PACS (EC 3.6. CATION-TRANSPORTING P-TYPE ATPASE B (EC CYANATE TRANSPORT PROTEIN CYNX DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPC zinc transport system membrane protein zinc-binding periplasmic protein precursor BRANCHED CHAIN AMINO ACID TRANSPORT SYSTEM 11 CARRIER PROTEIN Permeases CALCIUM/PROTON ANTIPORTER Ferrichrome transport proteins MACROLIDE-EFFLUX PROTEIN PUTATIVE 3-(3-HYDROXYPHENYL) PROPIONATE TRANSPORT PROTEIN PNUC PROTEIN Permeases Nucleic Acid Amino Acid Identification SEQ ID NO SEQ ID NO 605 606 RXN02566 607 608 F RXA02561 609 610 F RXA02566 611 612 RXA00051 613 614 RXAO1 172 615 616 RXA02128 Code Contig. NT Start NT Stop Function W0154 11823 13031 NUCLEOSIDE PERMEASE NUPG GR00732 664 5 NUCLEOSIDE PERMEASE NUPG GR00733 782 345 NUCLEOSIDE PERMEASE NUPG GROO008 5770 7173 PROLINE-SPECIFIC PERMEASE PROY GR00334 2687 4141 SULFATE PERMEASE GR00637 2906 4600 SULFATE PERMEASE Nudeic Acid SEQ ID NO 617 619 621 623 625 627 629 631 633 635 637 639 641 643 645 647 649 651 Amino Acid SEQ ID NO 618 620 622 624 626 628 630 632 634 636 638 640 642 646 648 650 652 Identification Code RXA02634 RXN02233 F RXA02233 RXN02372 F RXA02372 F RXA02377 RXA02676 RXN00432 F RXA00432 F RXA00436 RXA00847 RXN01382 F RXA01 382 RXA02659 RXN02933 RXN02991 RXN02992 RXN02996 Table 1 (continued) Con~t NT Start NT Stop Function GR00748 6045 7655 SULFATE PERMEASE W0068 6856 8142 URACIL PERMEASE GR00653 6856 8067 URACIL PERMEASE W0213 9311 11197 XANTHINE PERMEASE GR00688 6 560 XANTHINE PERMEASE GR00689 3336 4526 XANTHINE PERMEASE GR00754 2697 1309 GLUCONATE PERMEASE W0112 14751 13267 NA(+)-LINKED D-ALANINE GLYCINE PERMEASE GRO0lOD 1 891 NA(+)-LINKED D-ALANINE GLYCINE PERMEASE GROO101 45 569 NA(+)-LINKED D-ALANINE GLYCINE PERMEASE GR00230 1829 381 OLIGOPEPTIDE-BINDING PROTEIN APPA PRECURSOR (permnease) W01 19 8670 9761 OLIGOPEPTiDE-BINDING PROTEIN OPPA PRECURSOR GR00405 1067 6 OLIGOPEPTIDE-BINDING PROTEIN OPPA PRECURSOR (permease) GR00753 2 313 OLIGOPEPTIDE-BINDING PROTEIN OPPA PRECURSOR (permease) W01 76 30042 29233 DIPEPTIDE TRANSPORT SYSTEM PERMEASE PROTEIN DPPC W0072 618 4 GLUTAMINE TRANSPORT SYSTEM PERMEASE PROTEIN GLNP W0072 842 621 GLUTAMINE TRANSPORT SYSTEM PERMEASE PROTEIN GLNP W0069 1980 2648 HIGH-AFFINITY BRANCHED-CHAIN AMINO ACID TRANSPORT PERMEASE PROTEIN LIVH W01 12 9894 9001 TEICHOIC ACID TRANSLOCATION PERMEASE PROTEIN TAGG W01 12 21572 20769 MOLYBDATE-BINDING PERIPLASMIC PROTEIN PRECURSOR W01 12 20785 19949 MOLYBDENUM TRANSPORT SYSTEM PERMEASE PROTEIN MODB W0371 1 594 POTENTIAL STARCH DEGRADATION PRODUCTS TRANSPORT SYSTEM PERMEASE PROTEIN AMYD W01 16 2071 1142 POTENTIAL STARCH DEGRADATION PRODUCTS TRANSPORT SYSTEM PERMEASE PROTEIN AMYD RXN031 26 RXN00443 RXN00444 RXN00193 RXN01 298 661 662 Channel Proteins Nudeic Acid Amino Acid SEQ ID NO SEQ IDNO 663 664 665 666 667 668 669 670 671 672 Identification Code Contig. NT Start NT Stop Function RXA0 1737 RXN02348 RXA02426 RXN03164 RXN00024 GR00493 W0078 GR00707 W0277 W01 27 2913 6027 2165 1586 64219 3971 7910 633 2455 63275 POTASSIUM CHANNEL PROTEIN KUP SYSTEM POTASSIUM UPTAKE PROTEIN PROBABLE ANTI PORTER POTASSIUM CHANNEL BETA SUBUNIT POTASSIUM CHANNEL BETA SUBUNIT Lipoprotein and Li po polysaccha ride synthesis Nucleic Acid SEQ ID NO 673 675 Amino Acid Identification Code Contig.

SEQ ID NO 674 RXN01164 W0117 676 NT Start NT Stop Function 15894 14260 DOLICHOL-PHOSPHATE MANNOSYLTRANSFERASE (EC 2.4.1.83)1 APOLIPOPROTEIN N-ACYLTRANSFERASE (EC 14224 13415 DOLICHOL-PtiOSPHATE MANNOSYLTRANSFERASE (EC 2.4.1.83)1 APOLIPOPROTEIN N-ACYLTRANSFERASE (EC RXN01 168 W01 17 2 Excluded Genes GenBankTIu Gene Name Gene Function Reference Accession No.

A09073 ppg Phosphoenol pyruvate carboxylase Bachmann, B. et al. "DNA fragment coding for phosphoenolpyruvat corboxylase, recombinant DNA carrying said fragment, strains carrying the recombinant DNA and method for producing L-aminino acids using said Patent: EP 0358940-A 3 03/21/90 A45579, Threonine dehydratase Moeckel, B. et al. "Production of L-isoleucine by means of recombinant A45581, micro-organisms with deregulated threonine dehydratase," Patent: WO A45583, 9519442-A 5 07/20/95 A45585 AB0O3 132 murC; ftsQ; ftsZ Kobayashi, M. et al. "Cloning, sequencing, and characterization of the flsZ gene from coryneform bacteria," Biochen. Biophys. Res. Commun., (1997) ABO 15023 murC; ftsQ Wachi, M. et al. "A murC gene from Coryneform bacteria," App. Microbiol.

51(2):223-228 (1999) AB1853 0 dtsR Kimura, E. et al. "Molecular cloning of a novel gene, dtsR, which rescues the detergent sensitivity of a mutant derived from Brevibacterium Biosci. BiotechnoL Biochem.. 60(10):1565-1570 (1996) AB0 18531 dtsRlI; dtsR2 AB020624 murl D-glutamate racemase AB023377 tkt transketolase AB024708 gltB; gltD Glutamine 2-oxoglutarate am inotransfe rase and small subunits AB025424 acn aconitase AB0277 14 rep Replication protein AB02771 5 rep; aad Replication protein; aminoglycoside _________________adenyltransferase AF005242 argC A F005 63 5 ginA G lutam ine synthetase AF030405 hisF cyclase AF030520 argG Argininosuccinate synthetase AF03 1518 argF Ornithine carbamolytransferase AF036932 aroD 3-dehydroquinate dehydratase AF038548 pyc Pyruvate carboxylase 2 (continued) AF038651 dciAE; apt; rel Dipeptide-binding protein; adenine Wehmeier, L. et al. "The roie of the Corynebacterium glutamicum rel gene in phosphoribosyltransferase; GTP (p)ppGpp metabolism," Microbiology, 144:1853-1862 (1998) _________________pyrophosphokinase AF04 1436 argR Arginine repressor AF045998 impA Inositol monophosphate AF048764 argH Argininosuccinate AF049897 argC; argJ; argB; N-acetylIglutaxnylIphosphate reductase; argD; argF; argR; ornithine acetyltransferase; NargG; argH acetylgiutamnate kinase; acetylornithine transminase; ornithine carbamoyltransferase; arginine repressor; argininosuccinate synthase; argininosuccinate AF050109 inhA Enoyl-acyl carrier protein reductase_______ AF050166 hisG ATP phosphoribosyltransferase A FO51846 h isA Phosphoribo syl form im ino-5 -am ino- I phosphoribosyl-4-imidazolecarboxamide isomerase AF052652 metA Homoserine 0-acetyltransferase Park, S. et al. "Isolation and analysis of metA, a methionine biosynthetic gene encoding homoserine acetyltransferase in Corynebacterium glutamicum," Mol.

8(3):286-294 (1998) AF053071 aroB Dehydroquinate A F060558 h isH G lutamine AF086704 hisE Phosphoribosyl-ATP- AF 114233 aroA 5-enolpyruvylshikimate 3-phosphate AFI 16184 pan D L-aspartate-alpha-decarboxy lase precursor Dusch, N. et al. "Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli," AppI. Environ. MicrobioL, 65(4)1530- 1539(1999) A17124518 aroD; aroE 3-dehydroquinase; shikimate AF124600 aroC; aroK; aroB; Chorismate synthase; shikimate kinase; 3pepQ dehydroquinate synthase; putative cytoplasmic AF]45897 A17145898 2 (continued) AJO0 1436 ectP Transport of ectoine, glycine betaine, Peter, H. et "Corynebacterium glutamicum is equipped with four secondary proline carriers for compatible solutes: Identification, sequencing, and characterization of the proline/ectoine uptake system, ProP, and the ectoine/proline/glycine carrier, EctP," .1 Bacleriol, 180(22):6005-6012 (1998) AJ004934 dapD Tetrahydrodipicolinate succinylase Wehrmann, A. et "Different modes of diaminopimelate synthesis and their (incomplete') role in cell wall integrity: A study with Corynebacterium glutamicum," J Bacleriol, 180(1 2):3 159-3 165 (1998) AJ007732 ppc; secG; amt; ocd; Phosphoenol pyruvate-carboxy lase; high soxA affinity ammonium uptake protein; putative omnithine-cyclodecarboxylase; sarcosine oxidase AJO10319 ftsY, glnB, gInD; sip; Involved in cell division; PH1 protein; Jakoby, M. et al. "Nitrogen regulation in Corynebacterium glutamicum; amtP uridylyltransferase (uridylyl-removing Isolation of genes involved in biochemical characterization of corresponding enzmye); signal recognition particle; low proteins," FEMSMicrobiol, 173(2):303-310 (1999) ammonium uptake protein AJ1132968 cat Chioramphenicol aceteyl transferase AJ224946 mqo L-malate: quinone oxidoreductase Molenaar, D. et al. "Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium Eur. J. Biochem., 254(2):395-403 (1998) AJ238250 ndh NADH dehydrogenase AJ238703 porA Porin Lichtinger, T. et al. "Biochemical and biophysical characterization of the cell wall porin of Corynebacterium glutamicum: 'Mhe channel is formed by a low mass polypeptide," Biochemistry, 37(43):15024-15032 (1998) D1 7429 Transposable element IS3 1831 Vertes et al."lsolation and characterization of IS3 183 1, a transposable element Corynebacterium glutamicum," Mol. Microbiol., 1]l(4):739-746 (1994) D84 102 odhA 2-oxoglutarate dehydrogenase Usuda, Y. et al. "Molecular cloning of the Corynebacterium glutamicum (Brevibacterium lactofermentumn AJ 12036) odhA gene encoding a novel type 2-oxoglutarate dehydrogenase," Microbiology, 142:3347-3354 (1996) E01358 hdh; hk Homoserine dehydrogenase; homoserine Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent: TOP 1987232392-A 1 10/12/87 E01359 Upstream of the start codon of homoserine Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent: JP gene 1987232392-A 2 10/12/87 [E01375 operon E01376 trpL; trpE Leader peptide; anthranilate synthase Matsui, K. et al. "Tryptophan operon, peptide and protein coded thereby, utilization of tryptophan operon gene expression and production of Patent: JP 1987244382-A 1 10/24/87 2 (continued) E01377 Promoter and operator regions of Matsui, K. et at. "Tryptophan operon, peptide and protein coded thereby, tiyptophan operon utilization of tryptophan operon gene expression and production of Patent: JP 1987244382-A 1 10/24/87 E03937 Biotin-synthase Hatakeyama, K. et al. "DNA fragment containing gene capable of ceding synthetase and its utilization," Patent: JP 1992278088-A 1 10/02/92 E04040 Diamino pelargonic acid aminotransferase Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A 1 E04041 Desthiobiotinsynthetase Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A 1 E04307 Flavum aspartase Kurusu, Y. et al. "Gene DNA coding aspartase and utilization thereof," Patent: JP 1993030977-A 1 02/09/93 E04376 Isocitric acid lyase Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 3 03/09/93 E04377 Isocitric acid lyase N-terminal fragment Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 1993056782-A 3 03/09/93 E04484 Prephenate dehydratase Sotouchi, N. et al. "Production of L-phenylalanine by fermentation," Patent: JP 2 03/30/93 108 Aspartokinase Fugono, N. et al. "Gene DNA coding Aspartokinase and its use," Patent: JP 1993184366-A 1 07/27/93 E051 12 Dihydro-dipichorinate synthetase Hatakeyama, K. et al. "Gene DNA coding dihydrodipicolinic acid synthetase its use," Patent: JP 1993 184371 -A 1 07/27/93 E05776 Diaminopimelic acid dehydrogenase Kobayashi, M. et al. "Gene DNA coding Diaminopimelic acid dehydrogenase and its use," Patent: JP 1993284970-A 1 11/02/93 E05779 Threonine synthase Kohama, K. et al. "Gene DNA ceding threonine synthase and its use," Patent: JP 1993284972-A 1 11/02/93 E061 110 Prephenate dehydratase Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," J P 1993344881]-A 1 12/27/93 E061 I11 Mutated Prephenate dehydratase Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," JP 199334488 1-A 1 12/27/93 E06 146 Acetohydroxy acid synthetase Ilnui, M. et al. "Gene capable of ceding Acetohydroxy acid synthetase and its Patent: JP 1993344893-A 1 12/27/93 E06825 Aspartokinase Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A 1 E06826 Mutated aspartokinase alpha subunit Sugimoto, M. et "Mutant aspartokinase gene," patent: JP 1994062866-A I Table 2 (continued) E06827 Mutated aspartokinase alpha subunit Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A 1 E07701 secY Honno, N. et al. "Gene DNA participating in integration of membraneous to membrane," Patent: JP 1994 169780-A 1 06/21/94 E08177 Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase releasedfrominhibition and its utilization," Patent: JP 1994261766-A 1 09/20/94 E08 178, Feedback inhibition-released Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase released from E08179, feedback inhibition and its utilization," Patent: JP 1994261766-A 1 09/20/94 E08180, E0818 1, E08 182 E08232 Acetohydroxy-acid isomeroreductase Inui, M. et al. "Gene DNA coding acetohydroxy acid isomeroreductase," JP 1994277067-A 1 10/04/94 E08234 secE Asai, Y. et al. "Gene DNA coding for translocation machinery of protein," JP 1994277073-A 1 10/04/94 E08643 FT aminotransferase and desthiobiotin Hatakeyama, K. et al. "DNA fragment having promoter function in promoter region coryneform bacterium," Patent: JP 1995031476-A 1 02/03/95 E08646 Biotin synthetase Hatakeyama, K. et al. "DNA fragment having promoter function in bacterium," Patent: JP 1995031476-A 1 02/03/95 E08649 Aspartase Kohama, K. et al "DNA fragment having promoter function in coryneform Patent: JP 1995031478-A 1 02/03/95 £08900 Dihydrodipicolinate reductase Madori, M. et al. "DNA fragment containing gene coding Dihydrodipicolinate reductase and utilization thereof," Patent: JP 1995075578-A I 03/20/95 E08901 Diaminopimelic acid decarboxylase Madori, M. et al. "DNA fragment containing gene coding Diaminopimelic acid and utilization thereof," Patent: JP 1995075579-A 1 03/20/95 £12594 Serine hydroxymethyltransferase Hatakeyama, K. et al. "Production of L-trypophan," Patent: JP 199702839 I-A 1 02/04/97 E£12760, transposase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: E£12759, J P 1997070291 -A 03/18/97 E12764 Arginyl-tRNA synthetase; diaminopimelic Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: decarboxylase JP 1997070291 -A 03/18/97 E12767 Dihydrodipicolinic acid synthetase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: 1997070291-A 03/18/97 E12770 aspartokinase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291 -A 03/18/97 E12773 Dihydrodipicolinic acid reductase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: 1997070291 -A 03/18/97 2 (continued) E13655 Glucose-6-phosphate dehydrogenase Hatakeyama, K. et al. "Glucose-6-phosphate dehydrogenase and DNA capable coding the same," Patent: JP 199722466 1I-A 1 09/02/97 L01508 INvA Threonine dehydratase Moeckel, B. et al. "Functional and structural analysis of the threonine dehydratase of Corynebacterium glutamicum," J Bacterial, 174:8065-8072 L07603 EC 4.2.1.15 3-deoxy-D-arabinoheptulosonate-7- Chen, C. et al. "The cloning and nucleotide sequence of Corynebacterium phosphate synthase glutamicum 3-deoxy-D-arabinoheptulosonate-7-phosphate synthase gene," Micrablol Leta, 107:223-230 (1993) L09232 llvB; ilvN; ilvC Acetohydroxy acid synthase large subunit; Keilhauer, C. et al. "Isoleucine synthesis in Corynebacterium glutamicum: Acetohydroxy acid synthase small subunit; molecular analysis of the ilvB-ilvN-ilvC operon," J Bacteriol, 175(17):5595- Acetohydroxy acid isomeroreductase 5603 (1993) L 18874 PtsM Phosphoenolpyruvate sugar Fouet, A et al. "Bacillus subtilis sucrose-specific enzyme 11 of the phosphotransferase phosphotransferase system: expression in Escherichia coli and homology to enzymes 11 from enteric bacteria," PNAS USA, 84(24):8773-8777 (1987); Lee, 1.K. et al. "Nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme It and analyses of the deduced protein sequence," FEMS Microbial. Lea., 1 19(1-2):137-145 (1994) L27 123 ace13 Malate synthase Lee, H-S. et al. "Molecular characterization of aceB, a gene encoding malate synthase in Corynebacterium glutamicum," J Microbial Biotechnol., (1994) L27 126 Pyruvate kinase Jetten, M. S. et al. "Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum," App!. Environ. Microbial, 60(7):2501-2507 (1994) L28760 aceA Isocitrate lyase L35906 dtxr Diphtheria toxin repressor Oguiza, J.A. et al. "Molecular cloning, DNA sequence analysis, and characterization of the Corynebacterium diphtheriae dtxR from Brevibacterium _____________lactoferrnentum," J Bacterial., 177(2):465-467 (1995) M 13774 Prephenate dehydratase Follettie, M.T. et al. "Molecular cloning and nucleotide sequence of the glutamicum pheA gene," J Bacterial., 167:695-702 (1986) M 16175 5S rRNA Park, Y-H. et al. "Phylogenetic analysis of the coryneform bacteria by 56 sequences," J Bacterial, 169:1801-1806 (1987) M 16663 trpE Anthranilate synthase, 5' end Sano, K. et al. "Structure and function of the trp operon control regions of Brevibacterium lactofermentum, a glutamic-acid-producing bacterium," Gene, (1987) M 16664 trpA Tryptophan synthase, 3'end Sano, K. et al. "Structure and function of the trp operon control regions of Brevibacterium lactofermentum, a glutamic-acid-producing bacterium," Gene, (1987) 2 (continued) M25819 Phosphoenolpyruvate carboxylase O'Regan, M. et al. "Cloning and nucleotide sequence of the Phosphoenolpyruvate carboxylase-coding gene of Corynebacteriumn ____________glutarnicumn ATCC 13032," Gene, 77(2):237-251 (1989) 106 23S rRNA gene insertion sequence Roller, C. et al. "Gram-positive bacteria with a high DNA G+C content are characterized by a common insertion within their 23S rRNA genes," J Gen.

138:1167-1175 (1992) 107, 23S rRNA gene insertion sequence Roller, C. et al. "Gram-positive bacteria with a high DNA G+C content are 108 characterized by a common insertion within their 23S rRNA genes," J Gen.

Microbil, 138:1167-1175 (1992) M89931I aecD; brnQ; yhbw Beta C-S lyase; branched-chain amino acid Rossol, 1. et al. "The Corynebacterium glutamicum aecD gene encodes a C-S uptake carrier; hypothetical protein yhbw lyase with alpha, beta-elimination activity that degrades aminoethylcysteine," J Bacterial., 174(9):2968-2977 (1992.); Tauch, A. et al. "Isoleucine uptake in Corynebacterium glutamicum ATCC 13032 is directed by the bmnQ gene Arch Microbial, 169(4):303-312 (1998) S59299 trp Leader gene (promoter) Herry, D.M. et al. "Cloning of the trp gene cluster from a tryptophanhyperproducing strain of Corynebacterium glutamicum: identification of a mutation in the trp leader sequence," App. Enrviron. Microbial, 59(3):791-799 UI 11545 trpD Anthranilate phosphoribosyltransferase O'Gara, J.P. and Dun ican, L.K. (1994) Complete nucleotide sequence of the Corynebacterium glutamicum ATCC 21850 tpD gene." Thesis, Microbiology Department, University College Galway, Ireland.

U 13922 cgllM; cgllR; cIglIR Putative type 11 5-cytosoine Schafer, A. et al. "Cloning and characterization of a DNA region encoding a methyltransferase; putative type 11 stress-sensitive restriction system from Corynebacterium glutamicumn ATCC restriction endonuclease; putative type I or 13032 and analysis of its role in intergeneric conjugation with Escherichia type Ill restriction endonuclease coli," J Bacierial., 176(23):7309-7319 (1994); Schafer, A. et al. "The Corynebacterium glutamicum cgllM gene encoding a 5-cytosine in an McrBCdeficient Escherichia coli strain," Gene, 203(2):95-101 (1997) U 14965 recA U31224 px Ankri, S. et al. "Mutations in the Corynebacteriumn glutarnicumproline biosynthetic pathway: A natural bypass of the proA step," J Bacterial., (1996) U31225 proC L-proline: NADP+ 5-oxidoreductase Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline biosynthetic pathway: A natural bypass of the proA step," J Bacterial., 178(15):4412-4419 (1996) U3 1230 obg; proB; unkdh ?;gamma glutamyl kinase;similar to D- Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline isomer specific 2-hydroxyacid biosynthetic pathway: A natural bypass of the proA step," J. Bacterial, 178(1 5):4412-4419 (1996) (continued) U31281 bioB Biotin synthase Serebriiskii, "Two new members of the bio B superfamily: Cloning, sequencing and expression of bio B genes of Methylobacillus flagellatumn and glutamicum," Gene, 175:15-22 (1996) U35023 thtR; accBC Thiosulfate sulfurtransferase; acyl CoA Jager, W. et al. "A Corynebacterium glutamicum gene encoding a two-domain carboxylase protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins," Microbil., 166(2);76-82 (1996) U43535 cmr Multidrug resistance protein Jager, W. et al. "A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli," J Bacteril., 179(7):2449-2451I (1997) U43536 clpB Heat shock ATP-binding U53587 aphA-3 3'5' -aminoglycoside phosphotransferase U89648 Corynebacterium glutamicum unidentified sequence involved in histidine biosynthesis, sequence X04960 trpA; trpB; trpC; trpD; Tryptophan operan Matsui, K. et al. "Complete nucleotide and deduced amino acid sequences of trpE; trpG; trpL the Brevibacterium lactofermentum tryptophan operon," Nucleic Acids Res., 13.10 114 (1986) X07563 lys A DAP decarboxylase (meso..diaminopimelate Yeh, P. et al. "Nucleic sequence of the lysA gene of Corynebacterium9 decarboxylase, EC 4.1.1.20) glutamicum and possible mechanisms for modulation of its expression," Ml.

Genet., 212(1):112-119 (1988) X 14234 EC 4.1.1.31 Phosphoenolpyruvate carboxylase Eikmanns, B.J. et al. "The Phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: Molecular cloning, nucleotide sequence, and expression," Ml. Gen. Genet., 218(2):330-339 (1989); Lepiniec, L. et al.

"Sorghum Phosphoenolpyruvate carboxylase gene family: structure, function molecular evolution," Plant. Mol. Biol., 21 (3):487-502 (1993) Xl 17313 fda Fructose-b isphosp hate aldolase Von der Osten, C.H. et al. "Molecular cloning, nucleotide sequence and finestructural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutam icum fructose-I1, 6-biphosphate aldolase to class I and class 11 aldolases," Mol. Microbial., X53993 dapA L-2, 3-dihydrodipicolinate synthetase (EC Bonnassie, S. et al. "Nucleic sequence of the dapA gene from Corynebacterium glutam icum," Nucleic Acids Res., 18(2 1):6421I (1990) X54223 AttB-related site Cianciotto, N. et al. "DNA sequence homology between att B3-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum and the attP site of lambdacorynephage," FEMS. Microbil 66:299-302 (1990) X54740 argS; lysA Arginyl-tRNA synthetase; Diaminopimelate Marcel, T. et al. "Nucleotide sequence and organization of the upstream region decarboxylase of the Corynebacterium glutamicum lysA gene," Mol. Microbial., 4(11): 18 19- (1990) Table 2 (continued) X55994 trpL; trpE Putative leader peptide; anthranilate I-eery, D.M. et al. "Nucleotide sequence of the Corynebacterium glutamnicumn component I trpE gene," Nucleic Acids Res., 18(23):7 138 (1990) X56037 thrC Threonine synthase IHan, K.S. et al. "The molecular structure of the Corynebacterium glutamicum synthase gene," Mat. Microbial., 4(10):1693-1702 (1990) X56075 attB-related site Attachment site Cianciotto, N. et al. "DNA sequence homology between att B3-related sites of Corynebacterium diphtheriae, Corynebacteriumn ulcerans, Corynebacterium glutamicum and the attP site of lambdacorynephage," FEMS. Microbial, 66:299-302 (1990) X57226 lysC-alpha; lysC-beta; Aspartokinase-alpha subunit; Kalinowski, J. et al. "Genetic and biochemical analysis of the Aspartokinase asd Aspartokinase-beta subunit; aspartate beta from Corynebacterium glutamicum," Mat. Microbial., 5(5):1197-1204 (1991); semnialdehyde dehydrogenase Kalinowski, J. et al. "Aspartokinase genes lysC alpha and lysC beta overlap and are adjacent to the aspertate beta-semialdehyde dehydrogenase gene asd in ____________Corynebacterium glutamicum," Mat. Gen. Gene., 224(3):3 17-324 (1990) X59403 gap;pgk; tpi G lyceraId ehyde-3 -phosphate; Eikmanns, B.J. "Identification, sequence analysis, and expression of a phosphoglycerate kinase; triosephosphate Corynebacterium glutamicum gene cluster encoding the three glycolytic isomerase enzymes glyceralde hyde-3 -phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomeras," J Bacterial.. 174(1 9):6076-6086 (1992) X59404 gdh Glutamate dehydrogenase Bormann, E.R. et al. "Molecular analysis of the Corynebacteriumn glutamicum gdh gene encoding glutamate dehydrogenase," Mat. Microbial., 6(3):3 17-326 X603 12 lysI L-lysine permease Seep-Feldhaus, A.H. et al. "Molecular analysis of the Corynebacterium glutamicum lysI gene involved in lysine uptake," Mo!. Micrabiol., 5(12):2995- X66078 cop I Ps I protein Joliff, G. et al. "Cloning and nucleotide sequence of the csp I gene encoding PSI1, one of the two major secreted proteins of Corynebacterium glutamnicum: The deduced N-terminal region of PSI is similar to the Mycobacteriumn antigen complex," A6. Mlicrabial., 6(16):2349-2362 (1992) X661 12 glt Citrate synthase Eikmanns, 131. et at. "Cloning sequence, expression and transcriptional analysis of the Corynebacteriumn glutamicumn gItA gene encoding citrate synthase," Micrabiol., 140:1817-1828 (1994) X67737 dapB Dihydrodipicolinate reductase X69 103 csp2 Surface layer protein PS2 Peyret, J.L. et al. "Characterization of the cspB gene encoding PS2, an ordered surface-layer protein in Corynebacteriumn glutamicum," Mat. Microbial..

(1993) X69 104 153 related insertion element Bonamy, C. et al. "Identification of IS 1206, a Corynebacterium glutamicum 1S3-related insertion sequence and phylogenetic analysis," Mal. Microbial, (1994) 2 (continued) X70959 leuA Isopropylmalate synthase Patek, M. et al. "Leucine synthesis in Corynebacterium glutamicum: enzyme activities, structure of leuA, and effect of leuA inactivation on lysine Appi. Environ. Microbial, 60(l): 133-140 (1994) X71489 icd Isocitrate dehydrogenase (NADP+) Eikmanns, BT. et al. "Cloning sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme," J Bacterial, (1995) X72855 GDHA Glutamate dehydrogenase (NADP+) X75083, mtrA 5-methyltryptophan resistance Heery, D.M. et al. "A sequence from a tryptophan-hyperproducing strain of X70584 Corynebacterium glutamicum encoding resistance to Biophys. Res. Commun., 201(3):1255-1262 (1994) X75085 recA Fitzpatrick, R. et al. "Construction and characterization of recA mutant strains of Corynebacterium glutamicum and Brevibacterium lactofermentum," Appi.

BiolechnaL, 42(4):575-580 (1994) X75504 aceA; thiX Partial Isocitrate lyase; Reinscheid, D.J. et al. "Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme," J 176(1 2):3474-3483 (1994) X76875 ATPase beta-subunit Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit AnIonie Van Leeuwenhoek 64:285-305 (1993) X77034 tuf Elongation factor Tu Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit A nionie Van Leeuwenhoek, 64:2 85-305 (1993) X77384 recA Billman-Jacobe, H. "Nucleotid sequence of a recA gene from Corynebacterium glutamicum," DNA Seq., 4(6):403-404 (1994) X78491 aceB Malate synthase Reinscheid, D.i. et al. "Malate synthase from Corynebacterium glutamicum pta-ack operon encoding phosphotransacetylase: sequence analysis," 140:3099-3 108 (1994) X80629 16S rDNA 16S ribosomal RNA Rainey, F.A. et al. "Phylogenetic analysis of the genera Rhodococcus and Norcardia and evidence for the evolutionary origin of the genus Norcardia from within the radiation of Rhodococcus species," Microbia!., 14 1:523-528 X81 191 gluA; gluB; gluC; Glutamate uptake system Kronemeyer, W. et al. "Structure of the gluABCD cluster encoding the gluD glutamate uptake system of Corynebacterium glutam icum," J Baclerial, 177(5):1 152-1 158 (1995) X81379 dapE Succinyldiamninopimelate desuccinylase Wehrrnann, A. et al. "Analysis of different DNA fragments of Corynebacterium glutamnicum complementing dapE of Escherichia coli," 40:3349-56 (1994) 2 (continued) ddcdfo X82061 16S rDNA 16S ribosomal RNA Ruimy, R. et al. "Phylogeny of the genus Corynebacterium ddcdfo analyses of small-subunit ribosomal DNA sequences," Int. J Syst. Bacteriol., (1995) X82928 asd; lysC Aspartate-semialdehyde dehydrogenase; Serebrijski, 1. et al. "Multicopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," J.

1 77(24):7255-7260 (1995) X82929 proA Gamma-glutamyl phosphate reductase Serebrijski, 1. et al. "Multicopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," J Bacterial., 1 77(24):7255-7260 (1995) X84257 16S rDNA 16S ribosomal RNA Pascual, C. et al. "Phylogenetic analysis of the genus Corynebacterium based 16S rRNA gene sequences," Int. J Sys. Bacterial., 45(4):724-728 (1995) X85965 aroP; dapE Aromatic amino acid permease; Wehrmann et al. "Functional analysis of sequences adjacent to dapE of C.

glutamicum proline reveals the presence of aroP, which encodes the aromatic acid transporter," J Bacterial., 177(20):5991-5993 (1995) X86 157. argB; argC; argD; Acetylgiutamnate kinase; N-acetyl-gamma- Sakanyan, V. et al. "Genes and enzymes of the acetyl cycle of arginine argF; argi glutamyl-phosphate reductase; biosynthesis in Corynebacterium glutamicum: enzyme evolution in the early acetylornithine aminotransferase; ornithine steps of the arginine pathway," Microbiology, 142:99-108 (1996) carbamoyltransferase; glutamate N- X89084 pta; ackA Phosphate acetyltransferase; acetate kinase Reinscheid, D.i. et al. "Cloning, sequence analysis, expression and inactivation of the Corynebacteriumn glutamicum pta-ack operon encoding phosphotransacetylase and acetate kinase," Microbilogy, 145:503-513 (1999) X89850 attB Attachment site Le Marrec, C. et al. "Genetic characterization of site-specific integration functions of phi AAU2 infecting "Arthrobacter aureus C70," J Bacterial., 1996-2004 (1996) X90356 Promoter fragment Fl Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90357 Promoter fragment F2 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90358 Promoter fragment FIO Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90359 Promoter fragment F 13 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) 0 0 0 0 0

U'

-J

0 0 0 0 '.0 t..j 0'.

2 (continued) X90360 Promoter fragment F22 Patek, MI. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90361 Promoter fragment F34 Patek, MI. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90362 Promoter fragment F37 Patek, M. et al. "Promoters from C. glutamicum: cloning, molecular analysis search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90363 Promoter fragment F45 Patek, M. et al. "Promoters from Corynebacterium glutamnicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90364 Promoter fragment F64 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90365 Promoter fragment F75 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90366 Promoter fragment PFI10I Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90367 Promoter fragment PFI104 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90368 Promoter fragment PF109 Patek, M. et al. "Promoters from Corynebacterium glutamnicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X93513 amt Ammonium transport system Siewe, R.M. et al. "Functional and genetic characterization of the (methyl) ammonium uptake carrier of Corynebacterium glutamicum," J. Biol. Chem., (1996) X93514 betP Glycine betaine transport system Peter, H. et al. "Isolation, characterization, and expression of the Corynebacterium glutamicum betP gene, encoding the transport system for the solute glycine betaine," J Bacteriol., 178(17):5229-5234 (1996) X95649 orf4 Patek, M. et al. "Identification and transcriptional analysis of the dapB-ORF2dapA-ORF4 operon of Corynebacterium glutamicum, encoding two enzymes in L-lysine synthesis," Biolechnol. Lett., 19:1113-1117 (1997) X96471 lysE; lysG Lysine exporter protein; Lysine export Vrljic, M. et al. "A new type of transporter with a new type of cellular regulator protein function: L-lysine export from Corynebacterium glutamicum," Mol.

I Microbiol.. 22(5):815-826 (1996) Table 2 (continued) X96580 panB; panC; xyIB 3-methyr-2-oxobutanoate Sahm, H. et al. "D-pantothenate synthesis in Corynebacteriumn glutamicum and hydroxymethyltransferase; pantoate-beta- use of panBC and genes encoding L-valine synthesis for D-pantothenate alanine ligase; xylulokinase overproduction," Appi. Environ. Microbial., 65(5):1973-1979 (1999) X96962 Insertion sequence IS 1207 and transposase X99289 Elongation factor P Ramos, A. et al. "Cloning, sequencing and expression of the gene encoding elongation factor P in the amino-acid producer Brevibacterium lactofermentumn ____________(Corynebacterium glutamnicumn ATCC 13869)," Gene, 198:217-222 (1997) YOO 140 thrB Homoserine kinase Mateos, L.M. et al. "Nucleotide sequence of the homoserine kinase (thrB) gene the Brevibacteriumn lactofermentum," Nucleic Acids Res., 15(9):3922 (1987) Y00151 ddh Meso-diaminopimelate D-dehydrogenase Ishino, S. et al. "Nucleotide sequence of the meso-diamninopimelate D- (EC 1 .4.1.16) dehydrogenase gene from Corynebacterium glutamicum," Nucleic Acids Res., Y00476 thrA Homoserine dehydrogenase Mateos, L.M. et al. "Nucleotide sequence of the homoserine dehydrogenase (thrA) gene of the Brevibacteriumn lactofermnentum," Nucleic Acids Res., 15(24):10598_(1987) Y00546 hom; thrB Homoserine dehydrogenase; homoserine Peoples, O.P. et al. "Nuc leotide sequence and fine structural analysis of the kinase Corynebacterium glutamnicumn hom-thrB operon," Mol. Microbiol., 2(1):63-72 Y08964 murC; flsQ/divD; ftsZ UPD-N-acetylmuramate-alanine ligase; Honrubia, M.P. et al. "Identification, characterization, and chromosomal division initiation protein or cell division organization of the ftsZ gene from Brevibacteriumn lactofermentum," Mol. Gen.

protein; cell division protein Gene., 259(1):97-104 (1998) Y09 163 putP High affinity proline transport system Peter, H. et al. "Isolation of the putP gene of Corynebacteriumn glutamnicumproline and characterization of a low-affinity uptake system for solutes," Arch. Microbial., 168(2): 143-151 (1997) Y09548 pyc Pyruvate carboxylase Peters-Wendisch, P.G. et al. "Pyruvate carboxylase from Corynebacteriumn glutamicum: characterization, expression and inactivation of the pyc gene," 144:915-927 (1998) Y09578 leuB 3-isopropylmalate dehydrogenase Patek, M. et al. "Analysis of the leuB gene from Corynebacteriumn App!. Microbial. Biotechnol., 50(l):42-47 (1998) Y 12472 Attachment site bacteriophage Phi-16 Moreau, S. et al. "Site-specific integration of corynephage Phi- 16: The construction of an integration vector," Microbiol., 145:539-548 (1999) Y12537 proP Proline/ectoine uptake system protein Peter, H. et al. "Corynebacterium glutamicum is equipped with four secondary carriers for compatible solutes: Identification, sequencing, and characterization of the proline/ectoine uptake system, ProP, and the ectoine/proline/glycine carrier, EctP," J Bacteriol., 180(22):6005-6012 (1998) j 2 contined) Y 13221 gInA Glutamine synthetase I Jakoby, M. et at. "Isolation of Corynebacterium glutamicum gInA gene glutamine synthetase FEMS Microbial Let., 154(1):81-88 (1997) Y 16642 lpd Dihydrolipoamide dehydrogenase Y 18059 Attachment site Corynephage 304L Moreau, S. et al. "Analysis of the integration functions of φ304L: An module among corynephages," Virology, 255(1): 150-159 (1999) Z21501 argS; lysA Arginyl-tRNA synthetase; diaminopimelate Oguiza, J.A. et al. "A gene encoding arginyl-tRNA synthetase is located in the_ decarboxylase (partial) upstream region of the lysA gene in Brevibacterium lactofermentum: Regulation of argS-lysA cluster expression by arginine," J 1 75(22):7356-7362 (1993) Z21502 dapA; dapB Dihydrodipicolinate synthase; Pisabarro, A. et al. "A cluster of three genes (dapA, orf2, and dapB) of dihydrodipicolinate reductase Brevibacterium lactofermentumn encodes dihydrodipicolinate reductase, and a third polypeptide of unknown function," J Bacterial., 1 75(9):2743-2749 Z29563 thrC Threonine synthase Malumbres, M. et al. "Analysis and expression of the thrC gene of the encoded synthase," AppI. Environ. Microbial, 60(7)2209-2219 (1994) Z46753 16S rDNA Gene for 16S ribosomal RNA Z49822 sigA SigA sigma factor Oguiza, J.A. et al "Multiple sigma factor genes in Brevibacterium lactofermentum: Characterization of sigA and sigB," J Bacterial, 178(2):550- 553 (1996) Z49823 galE; dtxR Catalytic activity UDP-galactose 4- Oguiza, J.A. et al "The galE gene encoding the UDP-galactose 4-epimerase of epimerase; diphtheria toxin regulatory Brevibacterium lactofermentum is coupled transcriptionally to the dmdR gene," Gene, 177:103-107 (1996) Z49824 orfl1; sigB SigB sigma factor Oguiza, J.A. et al "Multiple sigma factor genes in Brevibacterium lactofermentum: Characterization of sigA and sigB," J Bacterial, 178(2):550- (1996) Z66534 Transposase Correia, A. et al. "Cloning and characterization of an IS-like element present in the genome of Brevibacterium lactofermentumn ATCC 13869," Gene, (1996) A sequence for tnis gene was published in the indicated reference. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.

WO 01/00805 WO 0100805PCT/IBOO/00926 81 TABLE 3: Corynebacterium and Brevibacterium Strains Which May be Used in the Practice of the Invention speie 4 ATC ER CDC, NCIM XCTC. DSJz Brevibacterium ammoniagenes 21054 Brevibacterium ammoniagenes 19350 Brevibacterium ammoniagenes 1 9351 Brevibacterium ammoniagenes 19352 Brevibacterium ammoniagenes 19353 Brevibacterium animoniagenes 19354 Brevibacterium ammoniagenes 19355 Brevibacterium ammoniagenes 19356 Brevibacterium ammoniagenes 21055 Brevibacterium ammoniagenes 21077 Brevibacterium ammoniagenes 21553 Brevibacterium ammoniagenes 21580 Brevibacterium ammoniagenes 39101 Brevibacterium butanicum 21196 Brevibacterium divaricatum 21792 P928 Brevibacterium flavum 21474 Brevibacterium flavum 21129 Brevibacterium flavum 21518 Brevibacterium flavum B 11474 Brevibacterium flavum B 11472.

Brevibacterium flavum 21127 Brevibacterium flavum 21128 Brevibacterium flavum 21427 Brevibacterium flavum 21475 Brevibacterium flavum 21517 Brevibacterium flavum 21528 Brevibacterium fiavum 21529 Brevibacterium flavum B 11477 Brevibacterium flavum BI 11478 Brevibacterium flavum 21127 Brevibacterium flavum BI 11474 Brevibacterium healli 15527 Brevibacterium ketoglutamicum 21004 Brevibacterium ketoglutamicum 21089 Brevibacterium ketosoreductum 21914 Brevibacterium lactofermentum Brevibacterium lactofermentum 74 Brevibacterium lactofermentum 77 Brevibacterium lactofernientum 21798 Brevibacterium lactofermentum 21799 Brevibacterium lactofermentum 21800 Brevibacterium lactofermentum 21801 Brevibacterium lactofermentum BI 14701 Brevibacterium lactofermentum BI 14711 WO 01/00805 WO 0100805PCT/IBOO/00926 82 YFRM IRRL CEC NC1f3:S4 C DSI .J .AIIAa P s w 3s -J YXRU_ Brevibacterium lactofermentumn 21086 Brevibacterium lactofermentumn 21420 Brevibacterium lactofermnentumn 21086 Brevibacteriumn lactofermentumn 31269 Brevibacterium linens 9174 Brevibacterium linens 19391 Brevibacterium linens 8377 Brevibacterium paraffinolyticumn 11160 Brevibacterium spec. Brevibacterium spec. Brevibacterium spec. 14604 Brevibacterium spec. 21860 Brevibacterium spec. 21864 Brevibacterium spec. 21865 Brevibacterium spec. 21 866 Brevibacterium spec. 19240 Corynebacterium acetoacidophilumn 21476 Corynebacterium acetoacidophilumn 13870 Carynebacterium acetoglutamnicumn B 11473 Corynebacterium acetoglutamnicumn B 11475 Corynebacterium acetoglutamnicumn 15806 Corynebacterium acetoglutamnicumn 21491 Coryneba cterium acetoglutamnicumn 31270 Corynebacterium acetophilum Corynebacterium ammoniagenes 6872 2399 Corynebacterium ammoniagenes 15511 Corynebacterium fujiokense 21496 Corynebacterium glutamnicumn 14067 Corynebacterium glutamnicumn 39137 Corynebacterium glutaniicum 21254 Corynebacterium glutamnicumn 21255 Corynebacterium glutamnicumn 31830 Corynebacterium glutamnicumn 13032 Corynebacterium glutainicum 14305 Corynebacterium glutamnicumn 15455 Corynebacterium glutamnicumn 13058 Corynebacterium glutamnicumn 13059 Corynebacterium glutamnicumn 13060 Corynebacterium glutamnicumn 21492 Corynebacterium glutamnicumn 21513 Corynebacterium glutamnicumn 21526 Corynebacterium glutamnicumn 21543 Corynebacteriumn glutamnicumn 13287 Corynebacterium glutamnicumn 21851 Corynebacterium glutamnicumn 21253 Corynebacterium glutarnicumn 21514 Corynebacterium Iglutamnicumn 21516 Corynebacterium Iglutamnicumn 21299 WO 01/00805 WO 0100805PCTIIBOO/00926 83 C~e nl-; ci~FT T -NIRL J.C IDIC[MB ICBS.R&. NGCjCDSMZ Corynebacterium glutamicum 21300 Corynebacterium glutamicum 39684 Corynebacterium glutamicum 21488 Corynebacterium glutamicum 21649 Corynebacterium glutamicum 21650 Corynebacterium glutamicum 19223 Corynebacterium glutamicum 13869 Corynebacterium glutamicum 21157 Corynebacterium glutaniicum 21158 Corynebacterium glutamicum 21159 Corynebacterium glutamicum 21355 Corynebacterium glutamicum 31808 Corynebacterium glutamicum 21674 Corynebacterium glutamicum 21562 Corynebacterium glutamicum 21563 Corynebacterium glutamicum 21564 Corynebacterium glutamicum 21565 Corynebacterium glutamicum 21566 Corynebacterium glutamicum 21567 Corynebacterium glutamicum 21568 Corynebacterium glutamicum 21569 Corynebacterium glutamicum 21570 Corynebacterium glutamicum 21571 Corynebacterium glutamicum 21572 Corynebacterium glutamicum 21573 Corynebacterium glutamicum 21579 Corynebacterium glutamicum 19049 Corynebacterium glutamicum 19050 Corynebacterium glutamicum 19051 Corynebacterium glutamicum 19052 Corynebacterium glutamicum 19053 Corynebacterium glutamicum 19054 Corynebacterium glutamicum 19055 Corynebacterium glutamicum 19056 Corynebacterium glutamicum 19057 Corynebacterium glutamicum 19058 Corynebacterium glutamicum 19059 Corynebacterium glutamicum 19060 Corynebacterium glutamicum 19185 Coynateriumi glutamicum 13286 Corynebacterium glutamicum 21515 Corynebacterium glutamicum 21527 Corynebacterium glutamicum 21544 Corynebacterium glutanicum 21492 Corynebacterium glutamicum B8 183 Corynebacterium glutamicum Corynebacterium glutamicum B12416____ Corynebacterium glutamicum B12417 WO 01/00805 WO 0100805PCTIBOOIOO926 84 C~iu ~ies ~e -ATCC FERM .~RLCEGJ -CM CBJNCCD M Corynebacterium glutamicum B 12418 Corynebacterium glutamicum BI 11476 Corynebacterium glutamicum 21608 Corynebacterium lilium P973 Corynebacterium nitnilophilus 21419 11594 Corynebacterium spec. P4445 Corynebacterium spec. P4446 Corynebacterium spec. 31088 Corynebacterium spec. 3 1089 Corynebacterium spec. 31090 Corynebacterium spec. 3 1090 Corynebacterium spec. 3 1090 Corynebacterium spec. 15954 20145 Corynebacterium spec. 21 857 Coryne bacterium spec. 21862 Corynebacterium spec. 21863 ATCC: American Type Culture Collection, Rockville, MD, USA FERM: Fermentation Research Institute, Chiba, Japan NRRL: ARS Culture Collection, Northern Regional Research Laboratory, Peoria, IL, USA CECT: Coleccion Espanola de Cultivos Tipo, Valencia, Spain NCIMB: National Collection of Industrial and Marine Bacteria Ltd., Aberdeen, UK CBS: Centraalbureau voor Schimmelcultures, Baarn, NL NCTC: National Collection of Type Cultures, London, UK DSMZ: Deutsche Sammlung von Mikroorganismen und Zellkulturen, Braunschweig, Germany For reference see Sugawara, H. et al. (1993) World directory of collections of cultures of microorganisms: Bacteria, fungi and yeasts 4 1h edn), World federation for culture collections world data center on microorganisms, Saimata, Japen.

Table 4: Alignment Results Lenath Accession Name of Genbank Hit ID length Genbank Hit

(NT)

1527 GB_-ITG3:AC009685 GBHTG3:AC009685 GB-HTG7:AC00951 1 rxa00091 876 GBBA1:D50453 GB.BA1 :SCI51 GB BA1 :ECOUW93 rxaD0092 789 GBBAI:5CH35 GBHTG3:ACO1 1498 0 GBHTG3:AC01 1498_ 0 rxaOOiO4 879 GBBA1:MTCY27O GSBPL2:T24M8 GBBA1:MTCY27O rxaOOl 13 5745 GB-BA1 :MAFASGEN GBBA1:BAFASAA GBBAi:MTCV159 rxa00164 1812 GBHTG2:H5J1153D9 GBHTG2:H5J1153D9 GBHTG2:H5J1153D9 rxaOOl8l 1695 GBBA1:CGPUTP GBBA2:U32814 GBBAl CGPLJTP rxa00186 870 GB-PR3AC004843 Source of Genbank Hit homology 210031 210031 271896 146191 40745 338534 45396 312343 312343 37586 68251 37586 10520 10549 33818 118360 118360 118360 3791 10393 3791 136655 AC009685 AC009685 AC00951 1 D50453 AL109848 U14003 AL07861 0 AC01 1498 AC0l 1498 Z95388 AF077409 Z95388 X87822 X64795 Z83863 ALl09806 AL109806 AL1 09806 Y09163 U32814 Y09163 ACOD4843 Homo sapiens chromosome 15 clone 91_E_13 map 15, -SEQUENCING IN PROGRESS 27 unordered pieces.

Homo sapiens chromosome 15 clone 91_-E_-13 map 15, -SEQUENCING IN PROGRESS 27 unordered pieces.

Homo sapiens clone RP1 1-8601313, SEQUENCING IN PROGRESS 59 unordered pieces.

Bacillus subtilis DNA for 25-36 degree region containing the amyE-srfA region, complete cds.

Streptomyces, coelicolor cosmid 151.

Escherichia coi K-12 chromosomal region from 92.8 to 00.1 minutes.

Streptomyces coelicolor cosmid H35.

Homo sapiens chromosome 19 clone CIT978SKB_50L1 7, SEQUENCING IN PROGRESS 190 unordered pieces.

Homo sapiens chromosome 19 clone CIT978SKB 50L17, SEQUENCING IN PROGRESS 190 unordered pieces.

Mycabacterlum tuberculosis H37Rv complete genome; segment 961162.

Arabidopsis thaliana BAC T24M8.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 96/162.

B.ammoniagenes FAS gene.

Bammoniagenes FAS gene.

Mycobacterium tuberculosis H37Rv complete genome; segment 111/162.

Homo sapiens chromosome 20 clone RP5-1 15309, -SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome 20 clone RP5-1 15309, -SEQUENCING IN PROGRESS In unordered pieces.

Homo sapiens chromosome 20 clone RP5-i 15309, -SEQUENCING IN PRO-GRESS in unordered pieces.

C.glutamicumn pulP gene.

Haemnophilus influenzae Rd section 129 of 163 of the complete genome.

C.glutamicum putP gene.

Homo sapiens PAC clone DJ0612F12 from 7p12-p14, complete sequence.

Homo sapiens Homo sapiens Homo sapiens Bacillus subtilis Streptomyces coelicolor A3(2) Escherchia coli Streptomyces coelicolor Homo sapiens Homo sapiens Mycobacterium tuberculosis Arabidopsis, thaliana Mycobacterium tuberculosis Corynebacteriurn ammoniagenes Corynebactenum ammonagenes Mycobacteriumn tuberculosis Homo sapiens Homo sapiens Homo sapiens Corynebacteriumn glutamicum Haemophilus influenzae Rd Corynebacteriumn glutamicum Homo sapiens

MGAP)

34,247 34,247 35,033 54,452 36,806 38,642 49.934 37,117 37,117 36,732 37,150 42,874 68,381 57.259 39,870 35,714 35.714 35.334 100,000 36,347 37,454 37.315 Date of Deposit 29-Sep-99 29-Sep-99 09-DEC-i1999 1 0-Feb-99 1 6-Aug-99 17-Apr-96 4-Jun-99 13-Dec-99 13-Dec-99 10-Feb-99 3-Aug-98 10-Feb-99 03-OCT-i1996 14-OCT-1 997 17-Jun-98 03-DEC-i1999 03-DEC-i1999 03-DEC- 1999 8-Sep-97 29-MAY-1998 8-Sep-97 5-Nov-98 GBHTG2:HS745114 GB-HTG2:HS74511 4 rxaO0l87 474 GBGSS1O:AQ184082 GB.GSS1 :CNS008ZZ GBGSSIO:AQ184082 rxa00201 292 GB_PR3:HSJ824lF16 GB BA1:RCSECA GBEST34:AV122904 rxa00228 714 GB-EST5:AA486042 GBEST15:AA486042 rxa00243 1140 GB_PR2:CNS01DS5 GB-HTG3:ACO1 1408 GBHTG3:ACO1 1408 rxa00259 2325 GB-HTG1:CEY62EiO GB-HTG1:CEY62EI0 GBPL2:YSCCHROMI rxa00269 912 GB-HTG4:AC009974 GB_-ITG4:AC009974 7508 rxa0O281 766 GB_BA1:SCE8 GB-BA1 :SCU51 332 133309 133309 506 1101 506 139330 2724 242 515 515 101584 79332 79332 254217 254217 41988 219565 219565 32050 24700 3216 AL033532 AL033532 AQ184082 AL052951 A01 84082 AL050325 X89411 AV1 22904 AA486042 AA486042 AL 121655 AC01 1408 AC01 1408 AL031 580 AL031 580 122015 AC009974 AC009974 ABO1 7508 AL035654 U51 332 Table 4 (continued) Homo sapiens chromosome 1 clone RP4-745114 map q23.1-24.3, Homo sapiens SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome 1 clone RP4-745114 map q23.1-24.3, Homo sapiens SEQUENCING IN PROGRESS'-. in unordered pieces.

HS-3216_Al _G08_17 CIT Approved Human Genomic Sperm Library 0 Homo Homo sapiens sapiens genomic clone Plate=3216 Col=1 5 Row=M. genomic survey sequence.

Drosophila melanogaster genome survey sequence T7 end of BAC Drosophila melanogaster BACR18L011 of RPCI-98 library from Drosophila melanogaster (fruit fly), genomic survey sequence.

HS_3216A1lG08,,17 CIT Approved Human Genomic Sperm Library 0 Homo Homo sapiens sapiens genomic clone Plate=3216 Col=1 5 Row--M, genomic survey sequence.

Human DNA sequence from clone 824F16 on chromosome 20, complete Homo sapiens sequence.

R.capsulatus DNA for secA gene. Rhodobacter capsulatus AV1 22904 Mus musculus C57BL/6J 10-day embryo Mus musculus cDNA clone Mus musculus 26105291-07, mRNA sequence.

ab40c08.rl Stratagene HeLa cell s3 937216 Homo sapiens cONA clone Homo sapiens IMAGE:843278 mRNA sequence.

ab40c08.rl Stratagene HeLa cell s3 937216 Homo sapiens cDNA clone Homo sapiens IMAGE:843278 mRNA sequence.

BAC sequence from the SPG4 candidate region at 2 p21-2p22. complete Homo sapiens Homo sapiens clone CIT978SKB 65D22, SEQUENCING IN. PROGRESS ~.Homo sapiens unordered pieces.

Homo sapiens clone CIT978SKB65D22. SEQUENCING IN PROGRESS ~,Homo sapiens unordered pieces.

Caenorhabditis elegans chromosome IV clone Y62E10, SEQUENCING IN Caenoriabdltls elegans PROGRESS In unordered pieces.

Caenorhabditis elegans chromosome IV clone Y62E10. SEQUENCING IN Caenorhabditis elegans PROGRESS in unordered pieces.

Saccharomyces cerevisiae chromosome I centromere and right arm sequence. Saccharomyces cerevisiae Homo sapiens chromosome unknown clone NH0459119. WORKING DRAFT Homo sapiens SEQUENCE, in unordered pieces.

Homo sapiens chromosome unknown clone NH0459119, WORKING DRAFT Homo sapiens SEQUENCE, in unordered pieces.

Bacillus halodurans C-125 genomic DNA, 32 kb fragment, complete cds. Bacillus halodurans Streptomyces coelicolor cosmid E8. Streptomyces coelicolor Streptomyces coelicolor histidine kinase homolog (absAll) and response Streptomyces coelicolor regulator homolog (absA2) genes, complete cds.

38,129 38,129 37.297 34.120 39,655 34,520 38,163 38.889 37,500 38,816 37.001 38,040 38.040 36,776 36,776 39,260 37,358 37,358 4,622 36,328 39,089 03-DEC-1999 03-DEC-i1999 1 -Nov-98 3-Jun-99 1 -Nov-98 23-Nov-99 6-Jan-96 1 -Jul-99 06-MAR-1 998 06-MAR-1998 29-Sep-99 06-OCT.1999 06-OCT-i1999 6-Sep-99 6-Sep-99 05-MAR-I1998 29-OCT-1999 29-OCT-i1999 14-Apr-99 1 1-MAR-1999 14-Sep-96 GBHTG4:ACO1 1122 187123 AC0l 1122 Homo sapiens chromosome 8 clone 23_-D -i9 map 8, SEQUENCING IN PROGRESS 27 ordered pieces.

Homo sapiens Hoo aies 38,658 14-OCT-1999 Table 4 (continued) Corynebadtenum glutamicum ectP gene. rxa00298 1968 GBBA1:CGECTP 2719 AJ001436 Corynebactedrn glutamicum Corynebacterium glutamicum rxaOO3A6 813 rxa00368 1698 rxa00369 817 rxaOG410 789 GBBAl :CGECTP GBEST24:A1234006 GBBA1:SC2E9 GB BA1 :SC9B1 GBBA1:ECU70214 GBBA2:AF065159 GBBA1 AEOCHIT1 GBEST4:D62996 GBBA1:YP1O2KB GBGSS8:A0012142 GBHTG2:ACOOSO81

GBBAIIATPLOCC

GBBA2:U67591 GBBA1:TIPOCCQMPJ 2719 AJ001436 Corynebacterium glutamicum ectP gene.

432 A1234006 EST230694 Normalized rat lung. Bento Soares Rattus sp. cDNA clone Rattus sp.

RLUCUO1 3Tend. mRNA sequence.

20850 AL021530 Streptomyces coelicolor cosmid 2E9. Streptomyces co 24800 AL049727 Streptomyces coellcolor cosmid 9B1. Streptomyces co 1231 71 U70214 Escherlchia coli chromosome minutes 4-6. Escherichia coli 35209 AF065159 Bradyrhizobium japonicum putative arylsulfatase (arsA), putative soluble lytic Bradyrhizobium transglycosylase precursor (sltA), dihydrodipicolinate synthase (dapA), MscL- japonicum (mscL), SmpB (smpB). BcpB (bcpB), RnpO (mpO), ReLAfSpoT homolog (re[A), PdxJ (pdxJ), and acyl carrder protein synthase AcpS (acpS) genes, complete ods; prokaryotic type I signal peptidase SipF (slpF) gene. sipF-sipS allele, complete cds; RNase III (mc) gene, complete cds; GTP-binding protein Era (era) gene, partial cds; and unknown genes.

6861 063139 Aeromonas sp. gene for chitinase. complete and partial cds. Aeromonas sp. 1 314 062996 HUM347GO1 B Clontech human aorta polyA+ mRNA (#6572) Homo sapiens Homo sapiens cDNA clone GEN-347G01 mRNA sequence.

119443 AL031866 Yersinia pestis 102 kbases unstable region: from 1ito 119443. Yersinia pestis 501 A0012142 8750HIA037010398 Cosmid library of chromosome 11 Rhodobacter sphaeroides Rhodobacter genomic clone 8750HlA037010398. genomic survey sequence. sphaeroides 180096 AC005081 Homo sapiens clone RG270D313, SEQUENCING IN PROGRESS 18 Homo sapiens unordered pieces.

8870 Z30328 A.tumefacdens Ti plasmid pTiAch5 genes for OccR, OccO, OccM, OccP. OccT. Agrobacterium OoxB, OoxA and ornithine cyclodeaminase. tumnefaciens 9829 U67591 Methanococcus jannaschii section 133 of 150 of the complete genome. Methanococcus jannaschii 4350 M80607 Plasmid pTiA6 (from Agribacterium tumefaciens) peniplasmic-type octopine Plasmid pTiA6 100.000 20-Nov-98 100,000 20-Nov-98 00 46,552 31-Jan-99 Q elicolor OS-24 43.267 4,613 39,490 40,409 28-Jan-98 27-Apr-99 21 -Sep-96 27-OCT-1999 38.577 1 3-Feb-99 41,.613 29-Aug-95 35,396 4-Jan-99 54,800 4-Jun-98 45.786 12-Jun-98 46,490 10O-OCT- 1994 45,677 28-Jan-98 46.490 24-Apr-96 rxaOO4l9 882 GB-BA2:MSU46844 16951 GBEST28:AI5 13245 471 GBHTG4:ACO1 0066 187240 permease (occR, occQ, occM, occP, and occJ) and lysR-type regulatory protein (occR) genes, complete cds.

U46844 Mycobactdr smegmatis catalase-peroxidase (katG), putative arabinosyl Mycobacterium transferase (embC, embA, embB). genes complete cds and putative propionyl- smegmatis coA carboxylase beta chain (pccB) genes, partial ods.

A1513245 GH1 3311.3prime GH Drosophila melanogaster head pOT2 Drosophila Drosophila melan melanogaster cONA clone GH1 331 1 3prime, mRNA sequence.

AC010066 Drosophila melanogaster chromosome 3L/72A4 clone RPCI98-2501, Drosophila melan SEQUENCING IN PROGRESS 70 unordered pieces.

Z99 118 Bacillus subtilis complete genome (section 15 of 21): from 2795131 to 3013540. Bacillus subtilis AL033396 C.albicans cosmid Ca35A5. Candida albicans AA336266 EST40981 Endometrial tumor Homo sapiens cONA 5Fend, mRNA sequence. Homo sapiens 57.029 gaster 37,696 gaster 39,607 49,810 35,041 39,733 1 2-MAY-i1997 16-MAR-1999 16-OCT-1999 26-Nov-97 5-Nov-98 O 21 -Apr-97 rxa00432 1608 GBBAI:BSUBOO1 5 GB-PL1 :CAC35A5 GBE5T1 3AA336266 218410 42565 378 rxa00449 1704 GB)ITG2:AC008199 124050 GBHTG2:ACOO81 99 124050 rxa00456 1500 rxa00477 1767 GB_RO:RATLNKP2 177 GB_GSS1:FR0030597 476 GBGSS5:AQ786587 556 GBGSS14:AQ526586 434 GB-EST17:AA610489 407 GB-PR1:HSH33G4 1015 GBEST3O:A1637667 579 GBHTG3:AC008708 83932 GBHTG3:AC008708 83932 GB _HTG3AC008708 83932 GBHTG1:I-SJ575L21 94715 GBHTG1:HSJ575L21 94715 Table 4 (continued) Doohl e AC008199 DrosophUa melanogaster chromosome 3 clone BACR01 K08 (D756) RPCI-98 Doohl e 01.K.8 map 94D-94D strain y; cii bw sp. -SEQUENCING IN PROGRESS 83 unordered pieces.

AC008199 Drosophila melanogaster chromosome 3 clone BACRO1 K08 (D756) RPCI1-98 Drosophila mel 01 .K.8 map 94D-940 strain y, cii bw sp, -SEQUENCING IN PROGRESS 83 unordered pieces.

M22337 Rat link protein gene, exon 2. Rattus sp.

AL026966 Fugu rubripes GSS sequence, cdone 091C22aF9, genomic: survey sequence. Fugu rubnipes AQ786587 HS_73086_Bi _HOS_MR CIT Approved Human Genomic Sperm Library 0 Homo Homo sapiens sapiens genomic clone Plate=3086 Col=-9 Row--P. genomic survey sequence.

A0526586 HS_5198_BiB03_SP6E RPCI-11 Human Male BAC Library Homo sapiens Homo sapiens genomic clone Piate=774 Col=5 Row--D, genomic survey sequence.

AA610489 np93e05.sl NCI_-CGAP -Thyl Homo sapiens cDNA clone IMAGE:1133888 Homo sapiens similar to gb:M1 1353 HISTONE H3.3 (HUMAN);, mRNA sequence.

X05857 Human H3.3 gene exon 4. Homo sapiens A1637667 ttl0gll1.x1 NCI CGAPGC6 Homo sapiens cDNA clone IMAGE:2240420 3. H-omo sapiens mRNA sequence.

AC008708 Homo sapiens chromosome 5 clone CIT978SKB_78F1. SEQUENCING IN Homo sapiens PROGRESS 12 unordered pieces.

AC008708 Homo sapiens chromosome 5 clone CIT978SKB_78F1, -SEQUENCING IN Homo sapiens PROGRESS 12 unordered pieces.

AC008708 Homo sapiens chromosome 5 clone CIT978SKB_78F1, -SEQUENCING IN Homo sapiens PROGRESS 12 unordered pieces.

AL096841 Homo sapiens chromosome 1 clone RP4-575L21, SEQUENCING IN Homo sapiens PROGRESS in unordered pieces.

AL096841 Homo sapiens chromosome 1 dlone RP4-575L21, SEQUENCING IN Homo sapiens PROGRESS in unordered pieces.

AC005960 Mus musculus chromosome 17 BAC citb20h22 from the MHC region, complete Mus musculus sequence.

AL 109848 Streptomyces coelicolor cosmid 151. Streptomyces anogaster 38.392 anogaster 38,392 40,678 47,407 38,406 36.951 41 .791 2-Aug-99 2-Aug-0 00 tA 27-Apr-93 25-Jun-98 3-Aug-99 1 1-MAY-1999 09-DEC-1 997 rxa00478 954 rxaOO48O 1239 38,182 24-Jan-96 35.417 27-Apr-99 38,769 3-Aug-99 38,769 3-Aug-99 00 36,797 3-Aug-99 38.138 23-Nov-99 38,138 23-Nov-99 38.712 01-DEC-1998 40,284 16-Aug-99 55.634 20-OCT-i1999 40,793 13-Jul-99 54,534 26-Nov-97 54,534 1-Jul-98 51.481 7-Feb-99 39,007 1-Jan-98 39,267 2-Apr-918 CN rxa00524 433 rxa00526 813 rxa00559 1140 GBRO.AC005960 GB_BA1:SC151 GBBA2:AF082879 GBBA1:B5P132617 GBBA1:BSUBOOO8 GB-BA2:AFO12285 GB BA1 :D90725 GBBA2:CAU77910 GB_EST4:H34952 GBBA2:AE000963 158414 40745 3434 5192 208230 46864 13796 AF082879 AJ132617 Z991 11 AF012285 D90725 Yerslnia enterocolitica ABC transporter enterochelin/enterobactin gene cluster complete sequence.

Burkholderia sp. P-transporter operon and flanking genes.

Bacillus subtilis complete genome (section 8 of 21): from 1394791 to 1603020.

Bacillus subtilis mobA-nprE gene region.

Eschenichia ooli genomic DNA. (19.7 20.0 min).

A3(2) Yersinia enterocolitica Burkholderia sp.

Bacillus subtIlis Bacillus subtilis Escherichia coli coelicolor 3385 U7791 0 Corynebacterium ammoniagenes sequence upstream of the 5-phosphoribosyl-1- Corynebacterium pyrophosphate amidotransferase (purF) gene. ammoniagenes 382 H34952 EST108261 Rat PC-12 cells, untreated Rattus sp. cDNA clone RPCCK07 similar Rattus sp.

to NADH-ubiquinone oxidoreductase complex I123 kDa precursor (iron-sulfur protein), mRNA sequence.

22014 AE000963 Archaeoglobus fulgidus section 144 of 172 of the complete genome. Archaeoglobus fu Igidus 38,338 15-DEC-1997 r-xa00570 852 rxa00571 1280 rxaOO59O 1288 rxaOO591 1476 rxa00596 576 rxaOO6O7 504 GBGSS12:AQ422451 563 GB_EST28:A1504741 568 GB,_EST18:AA712043 68 GBBA1:MTCY78 3381 GB PR3AC005788 3622 GBPR3:AC005338 3454 GB-HTG6:AC010932 2032 GBHTG6:AC010932 2032 GB.,BA1 .MSGB26CS 3704 GBIN1:CEK09E9 3w0 GBPR4:AF135802 4965 GBPR4:AF104256 4365 GBPR3:AC004659 1295 GBPR3:AC004659 1295 GBPRI:HUMCBP2 2047 GBBAl :MTVO1 0 340( GBBA1:MTVO10 340( Table 4 (continued) A0422451 RPCI-1 1 -185C3.TV RPCI-1 1 Homo sapiens genomic clone RPCI-1 1 -185C3, Homo sapiens genomic survey sequence.

A1504741 vIl 6c01 xl Stratagene mouse Tcell 937311 Mus musculus cDNA clone Mus musculus IMAGE:972384 3'similar to gb:Z14044 M.musculus mRNA for valosin-contalning protein (MOUSE):, mRNA sequence.

AA712043 vu29flO.rl Barstead mouse myotubes MPLRB5 Mus musculus cONA clone Mus musculus IMAGE:1 182091 5' similar to gb:L05093 60S RIBOSOMAL PROTEIN L18A (HUMAN);, mRNA sequence.

Z77165 Mycobacterium tuberculosis H37Rv complete genome; segment 145/162. Mycobacterium 38,767 23-MAR-1999 37,900 11 -MAR.1 999 42,647 24-DEC-1997 38.468 17-Jun-98 8 !4 AC005788 1i AC005338 ~73 AC01 0932 ~73 AC01 0932 tuberculosis Homo sapiens chromosome 19. cosmid R26652, complete sequence. Homo sapiens Homo sapiens chromosome 19, cosmid R31646, complete sequence. Homo sapiens Homo sapiens chromosome 15 clone RP11-296E22 map 15, SEQUENCING Homo sapiens IN PROGRESS 36 unordered pieces.

Homo sapiens chromosome 15 clone RP1 1-296E22 map 15, SEQUENCING Homo sapiens IN PROGRESS 36 unordered pieces.

Mycobacterium leprae cosmid B26 DNA sequence. Mycobacterium leprae Caenorhabditis elegans cosmid K09E9, complete sequence. Caenorhabditis elegans Homo sapiens thyroid hormone receptor-associated protein complex component Homo sapiens 36,911 36,911 37.242 06-OCT-i1998 30-Jul-98 30-Nov-99 36,485 30-Nov-99 I0 18 L78816 Z79602 AF1 35802 39.272 34,092 36,310 1 5-Jun-96 2-Sep-99 9-Apr-99 5 AF104256 577 577 AC004659 AC004659 D83174 AL02 1186 TRAP1 70 mRNA, complete cds.

H-omo sapiens transcriptional co-activator CRSP150 (CRSP15O) mRNA.

complete cds.

Homo sapiens chromosome 19, CIT-HSP-87m17 BAC clone, complete Homo sapiens chromosome 19, CIT-HSP-87m1 7 BAC clone, complete Human mRNA for collagen binding protein 2. complete cds.

Mycobacterium tuberculosis H37Rv complete genome; segment 119/162.

Homo sapiens Homo sapiens Homo sapiens Homo sapiens Mycobacterium tuberculosis Mycobacterium tuberculosis 36,617 4-Feb-99 34. 32 1 35,739 40,404 40,862 02-MAY- 1998 02-MAY-1998 6-Feb-99 23-Jun-99 AL021186 Mycobacterium tuberculosis H37Rv complete genome; segment 1191162.

38.833 23-Jun-99 rxa00623 1461 GBBAl :MTCY428 26914 Z81451 Mycobacterium tuberculosis H37Rv complete genome, segment 107/162.

GB_-BA1:RSPNGR234 34010 Z68203 GBBA2:AEO01O1 10057 AE000101 Rhizobiumn sp. plasmid NGR234a DNA.

Rhizobium sp. NGR234 plasmid pNGR234a, section 38 of 46 of the complete plasmid sequence.

Mycobacterium 60,552 tuberculosis Rhizobium sp. 51,992 Rhizobium sp. NGR234 51,992 17-Jun-98 8-Aug-96 12-DEC-1997 15-Nov-99 rxa00681 rxaOO69O 1269 GB-HTG5:AC008338 136685 AC008338 GB_HTG4AC009766 170502 AC009766 Drosophila melanogaster chromosome X clone BACR3OJO4 (D908) RPCI-98 Drosophila mel 30.J.4 map 19C-19E strain y; cii bw sp, -SEQUENCING IN PROGRESS 93 unordered pieces.

Homo sapiens chromosome 11 clone 404_-A_-03 map 11. ~SEQUENCING IN Homo sapiens PROGRESS 27 unordered pieces.

anogaster 35,341 37,984 19-OCT-1999 rxa00733 1008 GB_HTG4:AC009766 GB-EST3:AU054038 GBEST3O:AU054038 rxa00735 692 GBBA1:MTCY5O GBBA1:090904 GBBA1 090904 rxa00796 298 GBGSS14:AQ579838 GBPR4:AC007625 GBEST14:AA427576 756 GB-BA1:MTV022 GBRO:AC002109 GB-BA1 :MTV022 rxaOO8O2 837 GB _GSS14:A0563349 GB BA1 :DIHCLPBA GB_GSS3:B61538 rxa00819 1452 GBHTG3:AC008691_ GBHG1 A089 GBHTG3:AC008917 rxaOO82l 966 GB_HTG1:HS32B1 GBHTGI:-S32B1 GBPR3:AC004919 rxa00827 876 GB_EST6:W06539 GBPR4:AC008179 170502 245 245 36030 150894 150894 651 174701 580 13025 160048 13025 642 2441 698 110000 110000 186591 271488 271488 75547 300 181745 Table 4 (continued) AC009766 Homo sapiens chromosome 11 clone 404_-A_-03 map 11, -SEQUENCING IN Homo sapiens PROGRESS 27 unordered pieces.

AU054038 AU054038 Dictyostelium discoideum SL (H.Urushihara) Dictyostelium Dictyostelium d discoideum cONA clone SLK472, mRNA sequence.

AU054038 AU054038 Dictyostelium discoideum SL (H.Urushihara) Dictyostelium Dictyostelium d discoideum cONA clone SLK472, mRNA sequence.

liscoideum 43.265 liscoideumn 43,265 37.984 19-OCT.199 00 28-Apr-99 Z77137 D90904 090904 AQ579838 AC007625 AA427576 AL021 925 AC0021 09 AL021 925 A0563349 M32229 B61 538 AC008691 AC008691 AC009127 AL023693 AL023693 ACOD491 9 W06539 AC0081 79 Mycobacterium tuberculosis H37Rv complete genome; segment 55/162.

Synec*hocystis sp. PCC6803 complete genome, 6127, 630555-781448.

Synechocystis sp. PCC6803 complete genome, 6/27, 630555-781448.

T1 35342b shotgun sub-library of SAC clone 31 P06 Medicago truncatula genomic clone 31 -P-06-C-054, genomic survey sequence.

Genomic sequence of Homo sapiens clone 2314F2 from chromosome 18, complete sequence.

zw54b04.sl Soares -total -fetus_-Nb2HF8 -9w Homo sapiens cDNA done IMAGE:773839 3 similar to gb:M86852 PEROXISOME ASSEMBLY FACTOR-i (HUMAN);. mRNA sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 100/1 62.

Genomic sequence from Mouse 9. complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 100/162.

HS55335_2_A09_T7A RPCI-1 1 Human Male BAC Library Homo sapiens genomic clone Platesgi 1 Col=1 8 Row--B, genomic survey sequence.

BSnodosus cIpB gene encoding a regulatory subunit of ATP-dependent protease.

T1 7M1 7TR TAMVU Arabidopsis thaliana genomic clone T17M1 7, genomic survey sequence.

Homo sapiens chromosome 5 clone CIT978SKB_63A22, SEQUENCING IN PROGRESS 253 unordered pieces.

Homo sapiens chromosome 5 clone CIT978SKB_63A22, SEQUENCING IN PROGRESS 253 unordered pieces.

Homo sapiens chromosome 16 clone RPCI-1 1_498010, SEQUENCING IN PROGRESS 49 unordered pieces.

Homo sapiens chromosome 6 clone RP1 -321. -SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome 6 clone RP1-3211. SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens PAC clone D.108951323 from UL, complete sequence.

T2367 MVAT4 bloodstream form of serodemne WRATati .1 Trypanosoma brucei rtiodesiense cONA mRNA sequence.

Homo sapiens clone NH0576F01. complete sequence.

Mycobacterium tuberculosis Synechocystis sp.

Synechocystis sp.

Medicago truncatula Homo sapiens Homo sapiens Mycobacterium tuberculosis Mus musculus Mycobacterium tuberculosis Homo sapiens Dichelobacter nodosus Arabidopsis thaliana Homo sapiens Homo sapiens Homo sapiens Homo sapiens Homo sapiens Homo sapiens Trypanosoma brucei rhodesiense Homo sapiens 36,819 52,585 39,699 37,153 38,014 42.731 59,350 39.398 36,842 37,649 41,140 36,946 38,270 38,270 38,947 36.565 36.565 34.346 40.000 35,903 17-Jun-98 7-Feb-99 7-Feb-99 27-Sep-99 30-Jun-99 16-OCT-i1997 17-Jun-98 9-Sep-97 17-Jun-98 29-MAY-1999 26-Apr-93 21-Nov-97 3-Aug-99 3-Aug-99 3-Aug-99 23-Nov-99 23-Nov-99 19-Sep-98 12-Aug-96 28-Sep-99 GBEST18:AA710415 533 rxa00842 1323 rxa00847 1572 GBPR2:AC002379 GB R2:AC002379 GB_1N1:CEF02D8 GBQV:XELRDS38A GBHTG4:AC007920 118595 118595 31624 1209 234529 GBHTG4:AC007920 234529 rxaOO851 732 GBHTG2:AC004064 GBHTG2:AC004064 GBPR3:HSJ824F16 GB-HTG3:ACO1 0120 GBHTG3:AC01O 120 185000 185000 139330 12 1582 121582 Table 4 (continued) AA710415 vt53f08.rl Barstead mouse irradiated colon MPLRB7 Mus musculus cDNA clone Mus musculus IMAGE:1 166823 mRNA sequence.

AC002379 Human BAC clone GS165104 from 7q21, complete sequence. Homo sapiens AC002379 Human BAC clone GS165104 from 7q21, complete sequence. Homo sapiens Z7841 1 Caenorhabditis elegans cosmid F02D8, complete sequence. Caenortiabditis L79915 Xenopus laevis rdslperipherin (rds38) mRNA, complete cds. Xenopus laevis AC007920 Homo sapiens chromosome 3q27 coeRPCl1 1-208N14,~ SEQUENCING IN Homo sapiens PROGRESS 51 unordered pieces.

AC007920 Homo sapiens chromosome 3q27 clone RPCI1 1-208N14, *SEQUENCING IN Homo sapiens PROGRESS *,51 unordered pieces.

AC004064 Homo sapiens chromosome 4, **SEQUENCING IN PROGRESS ~,10 Homo sapiens unordered pieces.

AC004064 Homo sapiens chromosome SEQUENCING IN PROGRESS ~,10 Homo sapiens unordered pieces.

AL050325 Human DNA sequence from clone 8241716 on chromosome 20, complete Homo sapiens sequence.

AC010120 Drosophila melanogaster chromosome 3 clone BACR22N13 (D1 061) RPCI-98 Drosophila mel 22.N.13 map 96F-96F strain y; cn bw sp, "*SEQUENCING IN PROGRESS 83 unordered pieces.

ACO1O012O Drosophila melanogaster chromosome 3 clone BACR22N13 (D1061) RPCI-98 Drosophila mell 22.N.13 map 96F-96F strain y; cn bw sp, SEQUENCING IN PROGRESS 83 unordered pieces.

AC006898 Caenorhabditis elegans clone Y73B16x, -SEQUENCING IN PROGRESS 9 Caenorhabditis unordered pieces.

elegans 33,742 21-OCT-1999 39,833 9-Jul-98 39,833 9-Jul-98 39.833 23-Nov-99 41,562 24--DEC-1997 0 36,321 23-Jul-97 37,284 23-Jul-97 38,163 23-Nov-98 00 3gv'v4" 33,742 JU--juI-vt 21-OCT-1 999 rxa00852 813 anogaster 35,855 anogaster 36,855 elegans 36,768 24-Sep-99 24-Sep-99 24-Feb-99 GBHTG2:AC006898 299308 rxa00856 rxaOO87O 1635 rxaOO875 690 rxa00878 1986 GBBA1:STMMSDA GBPAT: 192043 GIBPAT:178754 GB -BA2:AF1 19715 GB_3A2:AE000372 GBBA1:ECU28375 GBHTG2:AC007472 GBHTG2:AC007472 GBHTG2:AC006798 3986 L48550 713 713 549 1 2144 55175 114003 192043 178754 AF1 19715 AE000372 U28375 AC007472 Streptomyces coelicolor methylmalonic acid semnialdehyde dehydrogenase Streptomyces coelicolor 63,743 (msdA) gene, complete cds.

Sequence 10 from patent US 5726299. Unknown. 38,850 Sequence 10 from patent US 5693781. Unknown. 38,850 Escherichia coi isopentyl diphosphate isomerase (idi) gene, complete cds. Escherichia coli 54,827 Escherichia coli K-12 MG1655 section 262 of 400 of the complete genome. Escherichia coli 51,416 Escherichia coli K-1 2 genome; approximately 64 to 65 minutes. Eschenichia coli 51,416 Drosophila melanogaster chromosome 2 clone BACR3OD19 (D587) RPCI-98 Drosophila melanogaster 36,592 30.D.19 map 49E-49F strain y; cii bw sp, SEQUENCING IN PROGRESS 79 unordered pieces.

Drosophila melanogaster chromosome 2 clone BACR3OD19 (0587) RPCI-98 Drosophila melanogaster 36,592 30.D.19 map 49E-49F strain y; cii bw sp, SEQUENCING IN PROGRESS 79 unordered pieces.

Caenorhabditis elegans clone Y51 F8, **SEQUENCING IN PROGRESS 30 Caenorhabditis elegans 36,699 unordered pieces.

09-MAY-i1996 01-DEC-1998 3-Apr-98 22-Apr-99 12-Nov-98 08-DEC-1995 2-Aug-99 2-Aug-99 25-Feb-99 114003 AC007472 207370 AC006798 rxa00880 1968 rxa00899 1389 rxa00902 1333 rxa00931 969 rxaOO94l 1440 nca00962 689 rxa0lO6O 1047 rxa01067 852 GBEST4:H22888 GBGSS13:A0426858 GB_PRi :A8002335 GBBA1.NGU58849 GBBA1:PLPDHOS GBBA1:PDGINTORF GBGSS15:A0606873 GBGSS9:AQ163442 GBPL1:P55T70 GB_GSS1:FR0025208 GB_GSS1:FR0021844 GBGSS12:A0403344 GBBA1:MTCY18O GBBA1 :MTCY18O GBBA2:MSGKATG GB-ITG6:ACO1 0998 GBGSS1:GGA340111 GBHTG6:AC010998 GBBA1:ECTTN7 GB_1N2:AF176377 GBGSS1O:A0196728 GB_BA1:U00016 GB_BA1:SYCGROESL GBBA1:090905 468 516 6289 2401 3119 6747 581 658 4974 612 252 593 44201 44201 1745 144338 990 144338 2280 8220 429 42931 3256 H22888 A0426858 AB002335 U58849 L06822 L06418 A0606873 A0163442 X69213 AL01 8047 AL01 4715 A0403344 Z97193 Z97193 L14268 ACO 10998 AJ232089 AC01 0998 A.JO01 816 AF1 76377 A01 96728 U00016 D1 2677 Table 4 (continued) ym54e12.rl Soares infant brain 1iNIB Homo sapiens cDNA clone IMAGE:52158 Homo sapiens mRNA sequence.

CITBI-El -2578F1 .TF CITBI-Ell Homo sapiens genomic clone 2578F1, genomic Homo sapiens survey sequence.

Human mRNA for KLAA0337 gene, complete cds. H-omo sapiens Neisseria gonorrhoeae piIS6 silent pilus locus. Neissenia gonorrhoeae Plasmid pSa (from Esctierichia coli) dilhydropteroate synthase gene, 3' end. Plasmid pSa Integron 1n7 (from Plasmid pOGO1 00 from Eschericiia col) integrase (int), Plasmid pDGO100 aminoglycoside adenylyltransferase (aad), quaternary ammonium compoundresistance protein. dihydrofolate reductase (dhfrX). and dilhydropteroate synthase (suit) genes.

HS_5404_B2_HOS_T7A RPCI-1 1 Human Male BAC Library Homo sapiens H-omo sapiens genomic clone Plate=980 Col=1 0 Row=P, genomic survey sequence.

nbxbOOO7AO7f CUGI Rice BAC Library Oryza sativa genomic clone Oryza sativa nbxbOO07AO7f, genomic survey sequence.

P.sativum Psst7O gene for heat-shock protein. Pisumn sativumn Frubripes GSS sequence, clone 14501 OaA8, genomic survey sequence. Fugu rubripes F.rubripes GSS sequence, clone 069K22aG5, genomic survey sequence. Fugu rubripes HS.2257_B_B03_T7C CIT Approved Human Genomic Sperm Library D Homo Homo sapiens sapiens genomic clone Plate=2257 CoI=5 Row--D, genomic survey sequence.

Mycobactenumr tuberculosis H37Rv complete genome; segment 85/162.* Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genome; segment 85/1 62. Mycobacteriumn tuberculosis Mycobacterium tuberculosis ethyl methane sulphonate resistance protein (katG) Mycobacterium gene, 3'end. tuberculosis Homo sapiens clone RP1 1-95116. **SEQUENCING IN PROGRESS ,17 Homo sapiens unordered pieces.

Gallus gallus anonymous sequence from Cosmid mapping to chromosome 2 Gallus gallus (Cosmid 34 Contig 15), genomic survey sequence.

Homo sapiens clone RPl 1-95116., SEQUENCING IN PROGRESS 17 H-omo sapiens unordered pieces.

Escherichia coli left end of transposon Tn7 including type 2 integron. Escherichia coli Caenortiabditis briggsae CES-1 (ces-1) gene, complete cds; and CPN-1 (cpn-1) Caenorhabditis briggsae gene, partial cds.

CIT-HSP-2381 F4.TR CIT-HSP Homo sapiens genomic clone 2381 F4, genomic Homo sapiens survey sequence.

Mycobactenumr leprae cosmid B1937. Mycobacteriumn leprae Synechocystis sp. groES and groEL genes. Synechocystis sp.

37,179 38,447 35,799 40.623 38,966 38,966 6-Jul-95 24-MAR-i1999 1 3-Feb-99 20-Jun-96 20-MAR-1996 20-MAR-1996 37,900 10-Jun-99 41.885 12-Sep-98 36.866 3-Jul-96 37,815 10-DEC-1997 37,698 10-DEC-i1997 31,552 13-MAR-1999 37,902 17-Jun-98 39,140 17-Jun-98 42,517 26-Aug-99 .39,497 08-DEC-1999 37,970 25-Aug-98 38,226 08-DEC-1999 38,822 4-Nov-97 39,921 09-DEC-1999 39,019 16-Sep-98 58.303 01-MAR-1994 34,593 3-Feb-99 34.593 7-Feb-99 139467 D90905 Synechocystis sp. PCC6803 complete genome, 7/27, 781449-920915. Synechocystis sp.

rxa01114 1347 GBBA1:PSEFAOAB 3480 14 GBB1PEAA 380 0390 P. fragi faoA and faoB genes, complete cds. Pedmnsfai 5 99 2Fb9 Pseudomonas fragi 51.919 2-Feb-99 GBBA1:AB014757 GBBAl :SC8D9 rxa01 136 555 GB-EST1 1:AA244557 GBEST14:AA407673 GB-EST26:A1390328 6057 Table 4 (continued) ABO14757 Pseudomonas sp. 61-3 genes for PhbR, acetoacetyl-CoA reductase, betaketothiolase and PHB synthase, complete cds.

Pseudomonas sp. 61-3 50,573 rxa01l138 540 GBOV:XLXINT1 GBPR4:AC006054 GBPR4AC006054 GBBAl SC E39 GBBA1:MSU50335 38681 AL035569 Streptomyces coelicolor cosmid 8D9. Streptomyces coelicolor 379 AA244557 mx07aOl .rl Scares mouse NML Mus musculus cONA clone IMAGE:679464 Mus musculus mRNA sequence.

306 AA407673 EST01834 Mouse 7.5 dpc embryo ectoplacental cone cDNA library Mus Mus musculus musculus cONA clone C0014F702 mRNA sequence.

604 A1390328 mx07aOl .yl Scares mouse NML Mus musculus cDNA clone IMAGE:679464 Mus musculus mRNA sequence.

1278 X13138 Xenopus laevis int-i mRNA for int-i protein. Xenopus laevis 143738 AC006054 Homo sapiens Xq28 BAC RPCI111-382P7 (Roswell Park Cancer Institute Human Homo sapiens BAC Library) complete sequence.

143738 AC006054 H-omo sapiens Xq28 BAC RPCI 11-382P7 (Roswell Park Cancer Institute Human Homo sapiens BAC Library) complete sequence.

23550 AL049573 Streptomyces coelicolor cosmid E39. Streptomyces coelicolor 5193 U50335 Mycobacterium smegmatis phage resistance (mpr) gene, complete cds. Mycobacterium 38,562 2-Aug-98 33.136 2-Feb-99 42,200 39,050 26-DEC-i 998 0

Q

26-Feb-99 10-MAR-1997 00 40,038 37,996 36,053 31 -MAR-i 995 1 -Apr-99 1 -Apr-99 rxaOii72 1578 62,357 31-MR-1999 37,853 1-Feb-97 GBBA1:BACTHRTRN 15467 D84213 Bacillus subtilis genome, trnl-feuABC region.

rxa01 191 1713 GBPR2:HS1191B2 60828 AL022237 Human DNA sequence from clone 1191832 on chromosome 22q13.2-13.3.

Contains part of the 81K (NBK, 8P4, BIPI) gene for BCL2-interacting killer (apoptosis-inducing). a 40S Ribososmal Protein S25 pseudogene and part of an alternatively spliced novel Acyl Transferase gene similar to C. elegans C5002.7. Contains ESTs, STSs, GSSs, two putative CpG islands and genomic marker D22Si 151, complete sequence.

60828 AL022237 Human DNA sequence from clone 119182 on chromosome 22q1 3.2-1 3.3.

Contains part of the 81K (NBK. BP4, BIPi) gene for BCL-2-interacting killer (apoptosis-inducing), a 40S Ribososmal Protein S25 pseudogene and part of an alternatively spliced novel Acyl Transferase gene similar to C. elegans C50D2.7. Contains ESTs, STSs, GSSs, two putative CpG islands and genomic marker 02251 151, complete sequence.

smegmatis Bacillus subtilis Homo sapiens Homo sapiens 53,807 6-Feb-99

CO

38,366 23-Nov-99 W~ GBPR2:HSi 19182 39,595 23-Nov-99 rxaO12O5 554 rxa0l212 1047 rxa01219 1005 GBBA1:MTCY373 GBPL1:ATY12776 GBPL2:AUT6K21 GB-BA2:SCD25 GBBA :SLGLYUB GB_-BA1:SCH1O GB PATA68024 GB_-PATA68025 GBPAT:A68027 35516 Z734 19 Mycobacterlum tuberculosis H37Rv complete genome; segment 57/162.

38483 99643 4 1622 2576 39524 520 193 193 Y1 2776 AL-021889 AL1 18514 X65556 AL049754 A68024 A68025 A68027 Arabidopsis thaliana DNA, 40 kb surrounding ACS1 locus.

Arabidopsis thaliana DNA chromosome 4, BAC clone T61<21 (ESSA project).

Streptomyces coelicolor cosmid 025.

Slividans tRNA-GlyU beta gene.

Streptomyces; coelicolor cosmid Hi10.

Sequence 19 from Patent W09743409.

Sequence 20 from Patent W09743409.

sequence 22 from Patent W09743409.

Mycobacterium tuberculosis Arabidopsis thaliana Arabidopsis thaliana Streptomyces coelicolor A3(2) Streptomyces lividans Streptomyces coelicolor unidentified unidentified unidentified 57,762 32,971 35,273 39,654 54,493 44.638 42,553 43,229 38.342 17-Jun-98 7-Sep-98 16-Aug-99 2 1-Sep-99 20-DEC-i1993 04-MAY-i1999 05-MAY-i1999 05-MY-1999 05-MAY-i1999 rxa01220 1200 GB_PR3:HS512Bi1 GBEST6:N99239 GB_ESTI 6.MA554268 rxa01l221 849 GBPR4:AF179633 GBVI:EHVU20824 GBBA2AEOOO4O7 rxa01222 822 GBPAT:AR068625 GBBA2:SSU51197 Table 4 (continued) 64356 AL031058 Human DNA sequence from clone 512B1 1 on chromosome 6p2 4 -25. Contains Homo sapiens the Desmoplakin I (DPI) gene, ESTs, STSs and GSSs, complete sequence.

424 N99239 zb76hl 1.s1 Soares-senescent-fibroblastsNbHSF Homo sapiens cDNA clone Homo sapiens IMAGE:309573 mRNA sequence.

400 AA554268 nk36c09.sl NCICGAPGC2 Homo sapiens cONA clone IMAGE: 101 5600 3' Homo sapiens similar to gb:X01 677 GLYCERALDEHYDE 3-PHOSPHATE DEHYDROGENASE, LIVER (HUMAN);, mRNA sequence.

96371 AF179633 Homo sapiens chromosome 16 map 16q23.3-q24.1 sequence. Homo sapiens 184427 U20824 Equine herpesvirus 2, complete genome. Equine herpesv 10601 AE000407 Escherichia coli K-12 MG1655 section 297 of 400 of the complete genome. Escherlchia col 28804 AR068625 Sequence 1 from patent US 5854034. Unknown.

28804 U51197 Sphingomonas S88 sphingan polysaccharide synthesis (spsG). (spsS), (spsR). Sphingomonas glycosyl transferase (spsQ), (spsl), glycosyl transferase (spsK). glycosyl transferase (spsL). (spsJ), (spsF), (spsD), (spsC), (spsE), Urf 32, Urf 26.

ATP-binding cassette transporter (atrO), ATP-binding cassette transporter (atrB), gluccsyl-isoprenylphosphate transferase (spsB), glucose-i -phosphate thymidylyltra nsfe rase (rhsA), d-TDP-"-eoxy-D-glucose -3,5-epimerase (rtisC) dTDP-D-glucose-4.6-dehydratase (rhsB), dTD P-6-eoxy-L-ma nnosedehydrogenase (rhsD). Urf 31, and Unf 34 genes, complete cds.

2791 U44918 Babesia bovis ATP-binding protein (babc) mRNA, complete cds. Babesia bovis 1800 Y16642 Corynebacteriumn glutamicum lpd gene, complete CDS. Corynebacteriu 35,478 23-Nov-99 0 39,623 20-Aug-96 00 36,111 8-Sep,-97

(A

virus 2 sp. S88 40,199 37.001 39,471 40,574 40,574 5-Sep-99 2-Feb-96 1 2-Nov-98 29-Sep-99 1&6MAY-I 996 rxa01260 1305 rxa0l26l 294 GBIN1:BBU44918 GBBA1:CGLPD GBBAl :MTVO38 GBPR3:AC005618 GBBA1:CGLPD GBI_HTG4:AC010045 GBHTG4:AC010045 GBBA2:AF125164 GBBA1 :AB002668 GBBA1:AB010415 GBSTS:AU027820 GBSTS:AU027820 16094 AL021933 Mycobacterium tuberculosis H37Rv complete genome; segment 24/162.

176714 AC005618 Homo sapiens chromosome 5. BAC clone 249h5 (LBNL H149), complete glutamicum Mycobacterium tuberculosis Homo sapiens Corynebacterium glutamicum m sequence.

Corynebacterium glutamicum lpd gene, complete COS.

39,228 9-Aug-97 99,923 1-Feb-99 59,056 17-Jun-98 36.270 5-Sep-98 100,000 1-Feb-99 1800 Y16642 164829 AC010045 164829 AC010D45 Drosophila melanogaster chromosome 3L75A1 clone RPCI98-17C1 7.

SEQUENCING IN PROGRESS 50 unordered pieces.

Drosophila melanogaster chromosome.3L[75Al clone RPCI98-17C1 7, SEQUENCING IN PROGRESS 50 unordered pieces.

Drosophila melanogaster 50,512 Drosophila melanogaster 50,512 rxa01 269 564 26443 24907 23112 238 238 AF125164 Bacteroides fragilis 638R polysaccharide B (PS B2) biosynthesis locus, complete Bacteroides fragilis 56,071 sequence; and unknown genes.

AB002668 Actinobacillus actinomycetemcomitans DNA for glycosyltra nsfe rase, lytic Actinobacillus 46,679 transglycosyiase, dTDP-4-rhamnose reductase, complete cds. actinomycetemcomitans ABO10415 Actinobacillus actinomycetemcomitans gene cluster for 6-deoxy-L-talan Actinobacillus 46,679 synthesis, complete cds. actinomycetemcomitans AU027820 Rattus norvegicus, OTSUKA clone, 0T78.02/918b07, microsatellite sequence, Rattus norvegicus 34,874 sequence tagged site.

AU027820 Rattus norvegicus. OTSUKA clone, 0T78.021918b07 microsatellite sequence. Rattus norvegicus 34,874 sequence tagged site.

16-OCT-i1999 16-OCT-1999 01-DEC-i1999 21-Feb-98 -3 13-Feb-99 02-AR-9 0 02-MvAR-1999 rxa01291 1056 rxa01292 1308 GBHTG3:AC006445 GBBA1:BSUBOO17 GBHTG3:AC010580 GBHTG3AC01 0580 GBGSS8:AQ001809 GBGSSBAQ001809 Table 4 (continued) 174547 AC006445 Homo sapiens chromosome 4, SEQUENCING IN PROGRESS 7 Homo sapiens 34,812 unordered pieces.

217420 Z99120 Bacillus subtilis complete genome (section 17 of 21). from 3197001 to 3414420. Bacillus subtilis 37,802 121119 AC010580 Drosophila melanogaster chromosome 3 clone BACR48J106 (01 102) RPCI-98 Drosophila melanogaster 35.637 48.J.6 map 96F-96F strain y; cn bw sp. SEQUENCING IN PROGRESS 71 unordered pieces.

121119 AC010580 Drosophila melanogaster chromosome 3 clone BACR48.1O6 (01 102) RPCI-98 Drosophila melanogaster 35.637 48.J.6 map 96F-96F strain y: cn bw sp, **SEQUENCING IN PROGRESS 71 unordered pieces.

705 AQ001809 CIT-HSP-229001 7.TF CIT-HSP Homo sapiens genomic clone 229001 7, Homo sapiens 42,021 genomic survey sequence.

705 A0001809 CIT-HSP-2290017.TF CIT-HSP Homo sapiens genomic clone 2290017, Homo sapiens 40,323 genomic survey sequence.

rxa01293 450 15-Sep-99 0 26-Nov-97 0 01-OCT-1999e 26-Jun-98 26-Jun-98 3-Jul-96 06-OCT-1 999 06-OCT-i1999 31 -0 T- 31-OCT-1999 29-Jan-98 1 5-Feb-97 15-Sep-99 rxa01339 1111 GBPL1:MGU60290 4614 GBHTG3:AC01 1371 .189187 GBHTG3:AC01 1371 189187 GBHTG4:AC009892 138122 GBHTG4:AC009892 138122 rxa01382 1192 GBPR3:AC00241 6 GBEST9:AA096601 128915 524 rxa01399 1142 GBE5T37:A1982114 626 U60290 Magnaporthe grisea nitrogen regulatory protein (NUTi) gene, complete cds. Magnaporthe g AC0l 1371 H-omo sapiens chromosome 5 clone CIT978SKB_107C20. SEQUENCING IN Homo sapiens PROGRESS 31 unordered pieces.

AC01 1371 Homo sapiens chromosome 5 clone CIT978SKB_107C20, SEQUENCING IN Homo sapiens PROGRESS 31 unordered pieces.

AC009892 Homo sapiens chromosome 19 clone CIT978SKB_83J4, SEQUENCING IN Homo sapiens PROGRESS 6 ordered pieces.

AC009892 Homo sapiens chromosome 19 clone CIT978SKB_83J4, SEQUENCING IN Homo sapiens PROGRESS 6 ordered pieces.

AC002416 Human Chromosome X, complete sequence. Homo sapiens AA096601 moO3bO9.rl Stratagene mouse lung 937302 Mus musculus clDNA clone Mus musculus IMAGE:552473 5' similar to gb:L06505 60S RIBOSOMAL PROTEIN L12 (HUMAN); gb:L04280 Mus musculus ribosomal protein (MOUSE);, mRNA A19821 14 pat.pk0074.e9.f chicken activated T cell cDNA Gallus gallus cDNA clone Gallus gallus pat.pkG074.e9.f 5 similar to H-ATPase B subunit, mRNA sequence.

U20766 Gallus gallus vacuolar H-ATPase B subunit gene, complete cds. Gallus gallus AC005690 Homo sapiens chromosome 4, SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

AC005690 Homo sapiens chromosome SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

AC006637 Caenorhabditis elegans clone F41 B4. SEQUENCING IN PROGRESS 1 Caenorhabditis unordered pieces.

AL020985 Caenorhabditis elegans chromosome V clone Y102G3, SEQUENCING IN Caenorhabditis AL020985 Caenorhabditis elegans chromosome V clone Y102G3, SEQUENCING IN Caenorhabditis AL031 113 Caenorhabditis elegans chromosome V clone Y1 1 3G7, SEQUENCING IN Caenorhabditis AF030975 Aeromonas salmonicida chaperonin GroES and chaperonin GroEL genes. Aeromonas sal complete cds.

AF030975 Aeromonas salmonicida chaperonin GroES and chaperonin GroEL genes, Aeromonas sal complete cds.

risea 38.707 39,741 39,741 40.154 40,154 37,521 40,525 37,785 rxa0l42O 1065 GB-IT:G2C0569 GBHTG2:AC005690 GB_-ITG2AC006637 1645 193424 193424 22092 38,244 07-DEC-I 995 37,44 1.1 -Apr-99 37,464 elegans 37,488 1 1-Apr-99 23-Feb-99 3-Dec-98 3-Dec-98 0 12-Jan-99 2-Apr-98 o rxa01467 414 rxa01576 882 GBHTG1;CEV1O2G3_ 110000 GB_-HTG1:CEY1O2G3_ 110000 GBHTG1:CEY113G7_ 110000 GBBA2:AF030975 2511 elegans elegans elegans monicida 35,437 35,437 35.437 41,516 GB-BA2:AF030975 2511 monicida 38,171 2-Apr-98 rxa01580 840 GBEST22.A1068560 GBGSSi4:AQ554460 GB_1N2:AC005449 GB_1N2:AC005449 965 681 85518 85518 A 1068560 AQ554460 AC005449 AC005449 rxa0l 584 rxa01604 771 rxa01614 1146 rxa01629 1635 rxa01644 1401 rxa01667 1329 rxa01722 1848 GBHTG3:AC01 1352 160167 GB HTG3ACO1 1352 160167 GB -HTG3:ACO 1402 168868 GBBA1:CGA224946 2408 GB-EST1 7AA608825 439 GB.YR4:AC0053T7 102311 GBBA1:CGPROPGEN 2936 GBBAl :CGPROPGEN 2936 GBPR4:AF191071 88481 GBBAl :MSGB577CO 37770

S

GBBA1:MLCB24O7 35615 121125 GBBA1:CGU43536 3464 GB-HTG4:AC009841 164434 GBHTG4.AC009841 164434 GBGSS1:FR0022586 522 GB-GSS1 :FR0022584 485 GBIN1:CET26H2 37569 AC01 1352 AC01 1352 AC01 1402 AJ224946 A608825 AC005377 Y1 2537 Y1 2537 AF191071 L01263 AL023596 AL0221 21 U43536 AC009841 AC009841 AL015452 AL01 5450 Z82055 Table 4 (continued) mgaeOOO3aCi 1If Magnaporthe grisea Appressorium Stage cDNA Library Pynicularia grisea cDNA clone mgaeOOO3aCl i f mRNA sequence.

RPCI-1 1-419F2.TV RPCI-1 1 Homo sapiens genomic clone RPCI-1 1-419F2, genomic survey sequence.

Drosophila melanogaster. chromosome 2R, region 4.4C4-44C5, P1 cdone DS06765, complete sequence.

Drosophila melanogaster, chromosome 2R, region 44C4-44C5. P1 clone DS06765, complete sequence.

Homo sapiens chromosome 5 clone CIT-HSPC_327F10, SEQUENCING IN PROGRESS 15 unordered pieces.

Homo sapiens chromosome 5 clone CIT-l-SPC_327F10, SEQUENCING IN PROGRESS 15 unordered pieces.

Homo sapiens chromosome 5 clone CIT978SKB_38B5, SEQUENCING IN PROGRESS unordered pieces.

Corynebactenium glutamicum DNA for L-Ma late:q ui none oxidoreductase.

af03gO7.sl Soares-testisNHT H-omo sapiens cDNA clone IMAGE: 1030620 3' similar to TR:G976083 G976083 HISTONE 1-2A RELATED. mRNA sequence.

Homo sapiens PAC clone DJ1 1 36G02 from 7q32-q34. complete sequence.

Cglutamicum proP gene.

Cglutamicum prop gene.

Homo sapiens chromosome 8 clone BAC 388006, complete sequence.

M. leprae genomic dna sequence. cosmid b577.

Mycobacterium Ieprae cosmid B2407.

Mycobacterium tuberculosis H37Rv complete genome; segment 1551162.

Corynebacterium glutamicum heat shock, ATIP-binding protein (clpB) gene, complete cds.

Drosophila melanogaster chromosome 31-177E1 clone RPC198-13F1 1, SEQUENCING IN PROGRESS 70 unordered pieces.

Drosophila melanogaster chromosome 3L177E1 clone RPCI98-i 3F1 1, SEQUENCING IN PROGRESS 70 unordered pieces.

F.rubripes GSS sequence, clone 077P23aB10, genomic survey sequence.

Frubripes GSS sequence, clone 077P23aB1 1, genomic survey sequence.

Caenorhabditis elegans cosmid T261-12, complete sequence.

Pyricularia grisea 40,073 Homo sapiens 36,522 Drosophila melanogaster 36,609 Drosophila melanogaster 33,612 09-DEC-1999 28-MAY-i1999 23-DEC-i1998 23-DEC-1998 Homo sapiens 33,688 Homo sapiens 33,688 Homo sapiens 33.688 Corynebacterium 42,284 glutamicumn Homo sapiens 40,092 Homo sapiens 37,811 Corynebacteriumn 100,000 glutamicumn Corynebacteriurn 100.000 glutamicumn Homo sapiens 35,612 Mycobacteriumn leprae 55,604 Mycobacterium leprae 36,416 Mycobactenumr 55,844 tuberculosis Corynebacterium 100,000 glutamicum Drosophila melanogaster 33.205 Drosophila melanogaster 33,205 Fugu rubripes 40,192 Fugu rubripes 35,876 Caenorhabditis elegans 34,759 06-OCT-1999 06-OCT-1999 06-OCT-i1999 11 -Aug -98 02-MAR-1998 28-Apr-99 17-Nov-98 17-Nov-98 11i-OCT-1999 1 4-Jun-96 27-Aug-99 24-Jun-99 1 3-MAR-i1997 16-0CT-1999 16-OCT-1 999 10-DEC-i1997 10-DEC-1997 c 19-Nov-99 rxa01727 1401 GBBA2:CORCSLYS 2821 M89931 rxa01737 1182 rxa01762 1659 rxa01764 1056 GBHTG6:AC011037 1037 GBBA1:SCGD3 GBHTG1:CNSO1 DSB GBHTG1:CNSO1DSB GB-BA1 :MTC128 GB-BA1 :SC6G10 GBBAi:5CE29 GBPL2:SPAC343 GB-PL2:SPAC343 rxa0l8Ol 1140 GBEST38:AW066306 GBGSS13:AQ484750 GBGSS1 3:AQ489971 rxa01823 900 GB-BA1:SC151 GBBA1:ECU82598 GBBA1:BSUBOO18 rxa01853 675 GBBA1:MTCY227 GBHTG3ACO1O189 GB_-ITG3:AC010189 rxa01l8 558 GBHTG4:AC01 1117 GBHTG4:AC01 1117 GBBA1:MTCY2B12 167849 167849 33779 222193 222193 36300 36734 26477 42947 42947 334 375 252 40745 136742 209510 35946 265962 265962 148447 148447 20431 AC011037 AC011037 AL096822 AL121 768 AL1 21 768 Z97050 AL049497 AL035707 ALl109739 AL109739 Table 4 (continued) Corynebacterium glutamicum beta C-S lyase (aecO) and branched-chain amino acid uptake carrier (brnQ) genes, complete cds. and hypothetical protein Yhbw (yhbw) gene, partial cds.

Homo sapiens clone RP1 1-7F18, WORKING DRAFT SEQUENCE, 19 unordered pieces.

Homo sapiens clone RP1 1-7F18, WORKING DRAFT SEQUENCE, 19 unordered pieces.

Streptomyces coelicolor cosmid GD3.

Homo sapiens chromosome 14 clone R-9761316, SEQUENCING IN PROGRESS in ordered pieces.

Homo sapiens chromosome 14 clone R-976B16, SEQUENCING IN PROGRESS in ordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 101162.

Streptomyces coelicolor cosmid 6G10.

Streptomyces coelicolor cosmid E29.

Spombe chromosome I cosmid c343.

Spombe chromosome I cosmid c343.

Corynebacterium glutamicum Homo sapiens Homo sapiens Streptomyces coelicolor Homo sapiens Homo sapiens Mycobacterium tuberculosis Streptomyces coelicolor Streptomyces coelicolor Schizosaccharomyces pombe Schizosaccharomyces pombe AW066306 AQ484750 AQ489971 ALl109848 U82598 Z99121 V'7724 AC0l10189 AC010189 ACOI 117 AC01 1117 Z81 011 36,903 687009003.yl 687 Early embryo from Delaware Zea mays cDNA, mRNA Zea mays sequence.

RPCI-1 1-248N4.TV RPCI-1 1 Homo sapiens genomic clone RPCI-1 1-248N4, Homo sapiens genomic survey sequence.

RPCI-1 1 -247N23.TV RPCI-1 1 Homo sapiens genomic clone RPCI- 1 -247N23, Homo sapiens genomic survey sequence.

Streptomyces coelicolor cosmid 151. Streptomyces coelicolor A3(2) Escherichia coli genomic sequence of minutes 9 to 12. Escherichia coli Bacillus subtilis complete genome (section 18 of 21): from 3399551 to 3609060. Bacillus subtilis Mycobacterlum tuberculosis H37Rv complete genome; segment 114/162. Mycobacterium tuberculosis Homo sapiens clone RPCI11-296K13, SEQUENCING IN PROGRESS ~.80 Homo sapiens unordered pieces.

Homo sapiens clone RPCII 1-296K13, SEQUENCING IN PROGRESS ~,80 Homo sapiens unordered pieces.

Homo sapiens chromosome 4 clone 173_C_09 map 4, SEQUENCING IN Homo sapiens PROGRESS 10 ordered pieces.

Homo sapiens chromosome 4 clone 173C09p4,~ SEQUENCING IN Homo sapiens PROGRESS 10 ordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 61/162. Mycobacterium tuberculosis 99,929 4-Jun-98 38,054 35,147 35,147 49,574 44,049 40.246 37,084 34.890 46,108 32.000 36.111 35,779 39,211 36.999 37.612 39,006 39,006 39,130 39,130 37.893 30-Nov-99 30-Nov-99 8-Jul-99 05-OCT-i1999 05-OCT-i1999 23-Jun-98 24-MAR-i1999 1 2-MAR-i1999 6-Sep-99 6-Sep-99 1 2-OCT-1999 24-Apr-99, 24-Apr-99 1 6-Aug-99 1 5-Jan-97 26-Nov-97 17-Jun-98 16-Sep-99 16-Sep-99 14-OCT-i1999 14-OCT-i1999 18-Jun-98 0 00 rxa01894 978 GB _BA1:MITCY274 39991 Z74024 GBjN1:CELF46H5 38886 U41543 GB-HTG3:AC009204 115633 AC009204 rxa01897 666 rxa01946 1298 =01~980 756 rxa01983 630 rxa02020 1111 rxa02O29 1437 GBHTG1:CEY48B6 GB-HTG1 :CEY486 GBHTG1 :CEY53F4_2 GB _BA1:MTV007 GB _BA1I:SC51F2A GB-BA1 :SCARD1GN GB-PL2:AC008262 GBPL1:ABOl 3388 GBJ'L1 :AB013388 GBHTG4:AC006467 GBJ-ITG4:AC006467 GB.,HTG4:AC006467 GBBAl :CGDNAAROF GBPAT:A58887 GBBAl :STYCARABA GBHTG2:AC003023 GBHTG2ACOO3O23 GBHTG2:HS118B18 293827 AL021151 293827 AL021151 110000 Z92860 32806 AL021 184 40105 AL049587 2321 X84374 99698 AC008262 73428 ABO1 3388 73428 ABO1 3388 175695 AC006467 Table 4 (continued) Mycobacterium tuberculosis H37Rv complete genome; segment 126/1 62.

Caenorhabditis elegans cosmid F46H5.

Drosophila melanogaster chromosome 2 clone BACRO3EI9 (031033) RPCI-98 03.E.19 map 36E-37C strain y; cni bw sp, **SEQUENCING IN PROGRESS 94 unordered pieces.

Caenorhabditis elegans chromosome 11 clone Y48136, -SEQUENCING IN PROGRESS in unordered pieces.

Caenorhabditis elegans chromosome 11 clone Y48B6, -SEQUENCING IN PROGRESS in unordered pieces.

Caenorhabditis elegans chromosome 11 clone Y53F4 -SEQUENCING IN PROGRESS in unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 64/162.

Streptomyces coelicolor cosmid 5F2A.

Scapreolus ardi gene.

Genomic sequence for Arabidopsis thaliana BAC F4N2 from chromosome 1.

complete sequence.

Arabidopsis thaliana genomic DNA, chromosome 5. TAC clone: K1 9E1, complete sequence.

Arabidopsis thaliana genomic DNA, chromosome 5, TAC clone: K19E1, complete sequence.

Drosophila melanogaster chromosome 2 clone BACRO3LO8 (D532) RPCI-98 Caenorhabditis elegans Caenor'abditis elegans Caenorhabditis elegans Mycobacterium tuberculosis Streptomyces coelicolor Streptomyces capreolus Arabidopsis thaliana Arabidopsis thaliana Arabidopsis thaliana 34,703 34,703 33.333 65,560 50.648 44,973 35,310 35,505 39,973 Mycobacterium 37.229 tuberculosis Caenorhabditis elegans 38,525 Drosophila melanogaster 31,579 19-Jun-98 29-Nov-96 !t 00 1-Au-99 1-Apr-99 15-Oct-99 17-Jun-98 24-MAY-1999 23-Aug-95 21-Aug-99 20-Nov-99 to 20-Nov-99 Drosophila melanogaster 36.672 175695 175695 )2612 1612 4378 104768 104768 104729 031L.8 map 40A-40C strain y: cn bw sp, SEQUENCING IN PROGRESS 9 unordered pieces.

AC006467 Drosophila melanogaster chromosome 2 clone BACRO3LO08 (0532) RPCI-98 Drosophila melanogaster 36,672 031L.8 map 40A-40C strain y; cn bw sp, SEQUENCING IN PROGRESS 9 unordered pieces.

AC006467 Drosophila melanvogaster chromosome 2 clone BACRO3LO8 (0532) RPCI-98 Drosophila melanogaster 32,367 031L.8 map 40A-40C strain y; cn bw sp, SEQUENCING IN PROGRESSo 9 unordered pieces.

X85965 Cglutamicum ORF3 and aroP gene. Corynebacterium 100,000 glutamicum A58887 Sequence 1 from Patent WO9701637. unidentified 100,000 M95047 Salmonella typhimurium transport protein, complete cds, and transfer RNA-Arg. Salmonella typhimurium 50.547 AC003023 Homo sapiens chromosome 11 clone pDJ363p2, SEQUENCING IN Homo sapiens 35,820 PROGRESS 22 unordered pieces.

AC003023 Homo sapiens chromosome 11 clone pDJ363p2, SEQUENCING IN Homo sapiens 35,820 PROGRESS 22 unordered pieces.

AL034344 Homo sapiens chromosome 6 clone RP1-1 18B1 8 map p24.1-25.3, Homo sapiens 34.355 SEQUENCING IN PROGRESS in unordered pieces.

27-OCT-i1999 27-OCT-1999 27-OCT-i1999 30-Nov-97 06-MAR-1998 1 3-MAR-1996 21-OCT-1997 21-OCT-i1997 03-DEC-1 999 Table 4 (continued) 63247 AC007695 Homo sapiens 12q24 BAC RPCI1i-124N23 (Roswell Park Cancer Institute rxa02030 1509 rxa02O73 1653 GBPR4:AC007695 GBPR4AC00r64 GBPR4:AC006464 GBBkA1:CGGOHA GB_BAi:CGGDH- GB _8A2:SCI-4 99908 99908 2037 2037 25970 AC006464 AC006464 X72855 X59404 AL031 514 Human BAC Library) complete sequence.

Homo sapiens BAC clone NH0436C1 2 from 2, complete sequence.

H-omo sapiens BAC clone NH0436C12 from 2. complete sequence.

C.glutamicum GDHA gene.

Corynebactenumr glutamicum, gdh gen for glutamate dehydrogenase.

Streptomyces coelicolor cosmid 2H4.

Homo sapiens Homo sapiens Homo sapiens Corynebacterium glutamicum Corynebacteriumn glutamicumn Streptomyces coelicolor A3(2) 38,681 35,445 35,968 39,655 44,444 38,452 1--Sep-99 22-OCT-1999 22-OCT.1999 24-MAY-1 993 30-Jul-99 1 9-OCT-i1999 rxa02O74 rxa02O95 1527 GBEST18:MA703380 471 rxaO2O99 373 rxa02115 1197 rxa02128 1818 rxa02133 329 GBHTG6AC009769 GBEST7:W70175 GBBA1:CAJ1O3i9 GB-HTG3:ACO1 1509 GBHTG3ACO1 1509 GBHTG5:AC010126 GBHTG5:AC010126 GBPR1:HUMHM145 GBBA1:MTCY19O GBBA1:MTCY19O GB GSS10:AQ 161109 GBBA2:MPAEOOOO58 122911 436 5368 111353 111353 175986 175986 2214 34150 34150 738 28530 AA703380 zjl2bO6.sl Soares-fetal liver s$pleen_1NFLS_-Si Homo sapiens cDNA clone Homo sapiens IMAGE:450035 3 similar to contains LTR5.t3 LTR5 repetitive element;, mRNA sequence.

AC0D9769 Homo sapiens chromosome 8 clone RP1 1-202112 map 8, LOW-PASS Homo sapiens SEQUENCE SAMPLING.

W70175 zd52cO2.rl Soares-fetal-heartNb-H19W Homo sapiens cDNA clone Homo sapiens IMAGE:344258 5, similar to contains LTR5.b2 LTR5 repetitive element;, mRNA sequence.

AJO10319 Corynebacterium glutamicum amtP, glnB, gInD genes and partial ftsY and srp CorynebacteriL genes. glutamicumn AC01 1509 Homo sapiens chromosome 19 clone CITB-H1_2189E23, SEQUENCING IN Homo sapiens PROGRESS 35 unordered pieces.

AC011509 Homo sapiens chromosome 19 clone CITB-H1_2189E23, SEQUENCING IN Homo sapiens PROGRESS 35 unordered pieces.

AC010126 Homo sapiens clone GS5021302, SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

AC010126 Homo sapiens clone GS502B02, SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

010925 Human mRNA for HM1 45. Homo sapiens Z70283 Mycobacteriumn tuberculosis H37Rv complete genome; segment 98/162. Mycobacterium tuberculosis Z70283 Mycobacterium tuberculosis H37Rv complete genome; segment 98/162. Mycobacterium' im 35,473 34,174 100,000 33,423 33,423 36.717 36,092 39,171 38,682 35,746 38,482 32,317 07-DEC-1999 16-OCT-1996 14-MAY-i1999 07-OCT-i1999 07-OCT-1 999 1 3-Nov-99 13-Nov-99 3-Feb-99 17-Jun-98 17-Jn9 1 2-Sep-98 18-Nov-96 4, 20-OCT-1999 36,518 24-DEC-1997 AQ161 109 nbxbOOO6003r CUGI Rice BAC Library Oryza sativa genomic clone nbxbOO06003r, genomic survey sequence.

AE000058 Mycoplasma pneumoniae section 58 of 63 of the complete genome.

AC008308 Drosophila melanogaster chromosome 3 clone BACRlOM16 (0743) RPCI-98 10.M.16 map 93C-93D strain y: cn bw ip, SEQUENCING IN PROGRESS 186 unordered pieces.

tuberculosis Oryza sativa Mycoplasma pneumoniae Drosophila melanogaster 34,579 GBHTG4:AC008308 151373 rxaO2lSO 924 rxaO2l7l 1776 rxa02173 1575 rxa02224 1920 rxa02225 905 rxa02233 1410 rxa02253 1050 GBHTG4:AC008308 151373 GBEST37:AWO1 2260 358 GBGSS3:B87734 389 GBPR4:AC005042 192218 GBBA2:AF010496 189370 GBE5T24:A1170522 367 GBPL1:PHVDLECA 1441 GBBA1:CGGLTG 3013 GBBA1:CGGLTG 3013 GBBA2:AEOO0iO4 10146 GBBA2:CXU21300 8990 GB-HTG3:AC009185 87184 GBHTG3:AC009185 87184 GB-BA2:MPAEOOO58 28530 GBEST26:A1337275 618 GBEST26:A1337275 618 GBBA1:ERWPNLB 1291 GBEST3O:AV021947 313 GBEST33:AV0871 17 251 GBEST11:AA25O21O 532 GBEST11:AA25O21O 532 Table 4 (continued) AC008308 Drosophila melanogaster chromosome 3 clone BACR1O0M16 (0743) RPCI-98 Drosophila melanogaster 34.579 10.M.16 map 93C-93D strain y; cn bw sp, **SEQUENCING IN PROGRESS 186 unordered pieces.

AW01 2260 umO~eO9.yl Sugano mouse kidney mkia Mus musculus cONA clone IMAGE:2182312 5 similar to SWAMPL_BOVIN P00727 CYTOSOL AMINOPEPTIDASE mRNA sequence.

B87734 RPCI 1 1-30D24.TP RPCI-1 1 Homo sapiens genomic clone RPCI-1 1-30024, genomic survey sequence.

AC005042 Homo sapiens clone NH0552E01, complete sequence.

AF010496 Rhodobacter capsulatus strain SB1 003, partial genome.

A1170522 EST216450 Normalized rat lung, Bento Soares Rattus sp. cONA clone RLUC075 3'end. mRNA sequence.

K03288 P.vulgaris phytohemagglutinin gene encoding erythroagglutinating phytohemnagglutinin complete cds.

X661 12 C.glutamicum glt gene for citrate synthase and ORF.

X661 12 C.glutamicum glt gene for citrate synthase and ORF.

AE000104 Rhizobium sp. NGR234 plasmid pNGR234a, section 41 of 46 of the complete plasmid sequence.

U21300 Corynebacterium striatumn hypothetical protein YbhB gene, partial cds; ABC transporter TetB (tetB). ABC transporter TetA (tetA), transposase, 23S rRNA methyltransferase, and transposase genes, complete cds; and unknown genes.

AC009185 Homo sapiens chromosome 5 clone CIT-I-SPC_248019, SEQUENCING IN PROGRESS 2 ordered pieces.

AC009185 Homo sapiens chromosome 5 clone CIT-HSPC_248019, SEQUENCING IN PROGRESS 2 ordered pieces.

AE000058 Mycoplasma pneumoniae section 58 of 63 of the complete genome.

A1337275 tb96h1 1.x1 NCICGAPCol6 Homo sapiens cONA clone IMAGE:2062245 3' similar to TR:015392 015392 ORF, COMPLETE COS. mRNA sequence.

A1337275 tb96hl lxi NCI_-CGAP_-Col16 Homo sapiens cDNA clone IMAGE: 2062245 3' similar to TR:Q15392 015392 ORF, COMPLETE CDS. mRNA sequence.

M65057 Erwlnia carotovora pectin lyase (pnl) gene, complete cds.

AV021947 AV021947 Mus musculus 18-day embryo C57BL/6J Mus musculus cDNA clone 11 90024M23, mRNA sequence.

AV087117 AV087117 Mus musculus tongue C57BLJ6J adult Mus musctilus cDNA clone 231 0028C1 5, mRNA sequence.

AA250210 mx79gl0.r0 Soares mouse NML Mus musculus cONA clone IMAGE:692610 5' similar to TR:E236517 E236517 F44G4.1 mRNA sequence.

AA250210 mx79gl0.rl Soares mouse NML Mus musculus cDNA clone IMAGE:692610 5' similar to TR:E236517 E236517 F44G4.1 mRNA sequence.

Mus musculus Homo sapiens Homo sapiens Rhodobacter caps ulatus Rattus sp.

Phaseolus vulgaris Corynebacterium glutamicum Corynebacterium glutamicum Rhizobium sp. NGR234 Corynebacterium striatumn Homo sapiens Homo sapiens Mycoplasma pneumoniae Homo sapiens Homo sapiens Erwinia carotovora Mus musculus Mus musculus Mus musculus Mus musculus 39,385 37,629 36,901 53,714 44,186 39,103 44,118 36,189 38,487 37,264 36,459 36,459 35,498 35,589 42,786 37,780 39,423 47,410 36,136 36,202 20-OCT-i1999 I OSe-9 0C 00 9-Apr-99 14-Jan-99 12-MAY-1998 20-Jan-99 27-Apr-93 1 7-Feb-95 17-Feb-95 1 2-DEC-1997 9-Apr-99 07-OCT-1 999 07-OCT-1999 1 8-Nov-96 18-MAR-1999 1 8-MAR-i1999 26-Apr-93 28-Aug-99 25-Jun-99 12-MAR-1997 12-MAR-i1997 rxa02261 1479 rxa02268 1023 rxa02269 1095 rxa02309 1173 rxa0231 0 1386 rxa02321 1752 rxa02335 1896 rxa02364 750 GB_BAl CGL007732 4460 GB_BAl :CGAMTGENE 2028 GB_BAl :CORPEPC 4885 GBPL2:AF087130 3478 GBEST3O:A1663709 408 GBRO:AF074714 3120 GBGSS4:A0742825 847 GBHTG3:AC009293 1629 GBHTG3:AC009293 1629 GBBA1.MTV25D1O 40831 GBBAl :MSGY224 40051 GBHTG2:AC007163 18661 GB_BA1:MTY25D1O 40831 GBBA1:MSGY224 40051 GB_PR3:HS279N11 1699~ GBBAI:AB018531 4961 G BPAT: E17019 4961 GBBAI.AB018530 2855 GB-BA1:CGU35023 3195 GB_BA1:U00012 33312 GB_BAl :MTCY71 42729 GBBAI:AP000006 3190( GBBA1:APOOOO6 3190( AJO07732 X93513 M25819 AF087130 A1663709 18 98 AF07471 4 AQ742825 AC009293 AC009293 Z95558 AD000004 AC0071 63 Z95558 AD000004 Z98255 AB0l 8531 El 17019 ABO1 8530 U35023 U00012 Z92771 Table 4 (continued) Corynebacterium glutamicum 3' ppc gene. secG gene, amt gene, ocd gene and Corynebacteriumn soxA gene. glutamicum C.glutamicumn amt gene. Corynebacteriumn glutamnicumn C.glutamicum phosphoenolpyruvate carboxylase gene, complete cds. Corynebacteriumn glutamnicumn Neurospora crassa siderophore regulation protein (sre) gene, complete cds. Neurospora crassa ud47a06.yl Soares mouse mammary gland NbMMG Mus musculus cONA clone Mus musculus IMAGE:1449010 5' similar to TR:075585 075585 MITOGEN- AND STRESS- ACTIVATED PROTEIN KINASE-2;, mRNA sequence.

Mus musculus mitogen- and stress-activated protein kinase-2 (mMSK2) mRNA, Mus muscu.lus complete cds.

HS_-5482_-B2_-A04_-T7A RPCI-1 1 Human Male BAC Library Homo sapiens Homo sapiens genomic clone Plate= 1058 CoI=-8 Row=B, genomic survey sequence.

Homo sapiens chromosome 18 clone 53 -I -06 map 18, SEQUENCING IN Homo sapiens PROGRESS -,15 unordered pieces.

Homo sapiens chromosome 18 clone 53 -I -06 map 18, SEQUENCING IN Homo sapiens PROGRESS 15 unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome: segment 28/162. Mycobacterium tuberculosis Mycobacteriumn tuberculosis sequence from clone y224. Mycobacterium tuberculosis Homo sapiens clone NHOO91MO5, **SEQUENCING IN PROGRESS 1 Homo sapiens unordered pieces.

Mycobactenumr tuberculosis H-37Rv complete genome: segment 28/162. Mycobacterium tuberculosis Mycobacteriumn tuberculosis sequence from clone y224. Mycobacteriumn tuberculosis Human DNA sequence from PAC 279N1 1 on chromosome Xqil1.2-13.3. Homo sapiens Corynebacterium glutamicum dtsRl and dtsR2 genes, complete cds. Corynebacteriumn glutamicumn Brevibacterium lactofermentumn dtsR and dtsR2 genes. Corynebacteriumn glutamicum Corynebacterium glutamicum dtsR gene, complete cds. Corynebacteriumn glutamicumn Corynebacterium glutamicumn thiosulfate sulfurtransferase (thtR) gene, partial Corynebactenumr cds, acyl CoA carboxylase (accBC) gene, complete cds. glutamicumn Mycobacterium leprae cosmid B1308. Mycobacterium Iepr Mycobacterium tuberculosis H37Rv complete genome; segment 1411162. Mycobactenum tuberculosis Pyrococcus horikoshii 0T3 genomic DNA: 1166001-1485000 nt. position (617). Pyrococcus horikos hii hii 100,000 100.000 100,000 39,268 41.523 38,347 37,703 37,006 37,006 52,344 52,344 37,263 36,861 36,861 34,516 99,030 98,973 99,030 99,947 40,247 67,568 36,130 34,543 7-Jan-99 29-MAY-1996 1 5-DEC-1995 22-OCT-i1998 10-MAY-1999 24-OCT-1998 1 6-Jul-99 13-Aug-99 1 3-Aug-99 17-Jun-98 03-DEC-i1996 23-Apr-99 1 7-Jun-98 03-DEC-i1996 23-Nov-99 19-OCT-1998 28-Jul-99 1 9-OCT-i1998 16-Jan-97 30-Jan-96 1 0-Feb-99 8-Feb-99 8-Feb-99 )0 AP000006 )0 AP000006 Pyrococcus horikoshii 0T3 genomic DNA, 1166001-1485000 nt. position (6W).

Pyrococcus horikos rxa02372 2010 GBHTG3:AC01 1461 100974 GB_HTG3AC01 1461 100974 GBEST2i:AA992021 279 GBTG4:AC009273 76175 GBHTG4:AC009273 76175 Table 4 (continued) AC0 11461 Homo sapiens chromosome 19 clone CIT-HSPC_429L19, -SEQUENCING IN Homo sapiens PROGRESS 4 ordered pieces.

AC01 1461 Homo sapiens chromosome 19 clone CIT-HSIPC _429L19, -SEQUENCING IN Homo sapiens PROGRESS 4 ordered pieces.

AA992021 ot36c0l.s1 Soares-testisNHT Homo sapiens cONA clone IMAGE: 1618848 Homo sapiens mRNA sequence.

AC009273 Arabidopsis thaliana chromosome 1 clone T1 N6. -SEQUENCING IN Arabidopsis th~ PROGRESS 2 ordered pieces.

AC009273 Arabidopsis thaliana chromosome 1 clone Ti SEQUENCING IN Arabidopsis th~ 36,138 07-OCT-1999 36,138 07-OCT-1999 41,219 3-Jun-98 0 rxa02397 1119 aliana aliana GB..BA1 :090826 rxa02424 723 GBEST13:AA334108 GBPR3:AC005224 GBPR3AC005224 rxa02426 1656 GBPAT:A06664 GBPAT:A04115 GB-BA1 :BACLDHL 19493 275 166687 166687 1350 090826 AA3341 08 AC005224 AC005224 A06664 PROGRESS 2 ordered pieces.

E.coli genomic DNA, Kohara clone #335(40.9-41.3 min).

EST38262 Embryo, 9 week H-omo sapiens cONA 5' end, mRNA sequence.

Homo sapiens chromosome 17, clone hRPK.214_0_1, complete sequence.

Homo sapiens chromosome 17, clone hRPK.21401, complete sequence.

B.stearothermophilus Ict gene.

rxa02487 1827 GBBA2AFOO71O1 GBBA1:MTC1364 GB_BA2AF1 19621 1361 A041 15 B.stearothermophilus recombinant Ict gene.

1361 M14788 B.stearothermophilus Ict gene encoding L-lactate dehydrogenase. complete cds.

32870 AF0071 01 Streptomyces hygroscopicus putative pteridine-dependent dioxygenase, PIKS modules 1,2,3 and 4, and putative regulatory protein genes, complete cds and putative hydroxylase gene, partial cds.

29540 Z93777 Mycobacteriumn tuberculosis H37Rv complete genome; segment 52/162.

Escherichia coli Homo sapiens Homo sapiens Homo sapiens Bacillus stearothermophilus synthetic construct Bacillus stearothermophilus Streptomyces hygroscopicus Mycobacterium tuberculosis Pseudomonas abietaniphila Homo sapiens 40,042 17-Feb-97 40,338 26-Apr-93 38.566 38,566 39.600 38,603 36,111 33,427 39,936 12-OCT-i1999 12-OCT-i1999 21-MAR-i1997 21-Apr-97 14-Aug-98 14-Aug-98 29-Jul-93 15986 AF119621 235395 AC002470 Pseudomonas abietaniphila BKME-9 DOt (ditl), dioxygenase DitA oxygenase component small subunit (ditA2), dioxygenase DitA oxygenase component large subunit (ditAl), DitH (ditH), 0itG (ditG), Oitf (ditF). DitR (ditR), DitE (ditE), DitD (ditO), aromatic diterpenoid extradiol ring-cleavage dioygenase (ditC), OitB (ditB), and dioxygenase DitA ferredoxin component (ditA3) genes, complete cds: and unknown genes.

Homo sapiens Chromosome 22ql 1.2 BAC Clone b135116 In BCRL2-GGT 43,298 44,352 43.611 37,971 13-Jan-98 17-Jun-98 28-Apr-99 30-Nov-99 rxaO2511 780 GB-PR4AC002470 GBPR4:AC002472 GB-EST34:A806938 GBBA1:MTCY1AiO GB BA1 :MLCL581 GBOV:GGU43396 GBBA2:AF008220 147100 118 25949 Region, complete sequence.

AC002472 Homo sapiens Chromosome 22q1 1.2 PAC Clone pn5 In BCRL2-GGT Region, Homo sapiens complete sequence.

A1806938 wf24b07.xi Soares_-NFL_-T_-GBC_-Si H-omo sapiens cDNA clone Homo sapiens IMAGE:2356501 3'similar to SW:PLZF-HUMAN 005516 ZINC FINGER PROTEIN PLZF mRNA sequence.

Z95387 Mycobacterium tuberculosis H37Rv complete genome; segment 117/162. Mycobacterium 38,239 13-Sep-99 38.983 7-Jul-99 rxa02512 1086 rxa02527 1452 36225 Z96801 2738 U43396 220060 AF008220 Mycobacterium leprae cosmid L581.

Gallus gallus tropomnyosin receptor kinase A (ctrkA) mRNA, complete cds.

Bacillus subtilis rrnB-dnaB genomic region.

tuberculosis Mycobacteriumn leprae Gallus gallus Bacillus subtilis 37,407 43,193 38,789 37,395 17-Jun-98 24-Jun-97 18-Jan-96 4-Feb-98 Table 4 (continued) 220060 AF008220 Bacillus subtilis rmB-dnaB genomic region.

rxa02547 2262 rxa02566 1332 rxa02571 1152 rxa02578 1227 rxa02581 1983 GBBA2:AF008220 GBHTG2:AC005861 GBPL1:AB006530 GBPL1:CNASA GB..PL1 :AB006530 GBEST32:Al727189 GBBA1:CGPUTP GBPL2:SPAC13G6 GBBAl :CGU43535 GBE5T35:A1857385 GB. Ml :CGU43535 GBPL1:AB016871 GBPL1 :AB025602 GBIN1:CELF36H9 GBBA1:MTVOO5 GBMl :MTV0O5 GBBA1:MTVO26 GBBA1:MTCY338 GBBA1:SEERYABS GBBA2:AF1 13605 GB BAI:SC1 C2 GBBA1:AB018531

GBBAI:AEMML

Bacillus subtilis 112369 7344 5729 7344 619 3791 33481 2531 488 2531 79109 55790 35985 37840 37840 23740 29372 20444 1593 42210 4961 2585 AC005861 ABOO6530 D85624 AB006530 A17271 89 Y09163 Z54308 U43535 A1857385 U43535 AB016871 AB025602 AF016668 AL01 01 86 AL01186 AL022076 Z74697 X62569 AF113605 AL031 124 ABO 18531 X99639 Arabidopsis thaliana clone F23B324,. SEQUENCING IN PROGRESS Arabidopsis thaliana unordered pieces.

Citrullus lanatus Sat gene for serine acetyltransferase. complete eds and Citrullus lanatus flanking region.

Citrullus vulgaris serine acetyltransferase (Sat) DNA, complete cds. Citrullus lanatus Citrullus lanatus Sat gene for serine acetyltransferase, complete cds and Citrullus lanatus flanking region.

BNLGHi7498 Six-day Cotton fiber Gossypium hirsutum cDNA 5' similar to Gossypium hirsutum (AB020715) KIAA0908 protein [Homo sapiens), mRNA sequence.

C.glutamicum putP gene. Corynebacterium glutamlcum S.pombe chromosome I cosmid ci 3G6. Scliizosaccharomyces pombe Corynebacterium glutamicum muftidrug resistance protein (cmr) gene, complete Corynebactenium cds. glutamicum wl55e03.xl NCI_-CGAPBrn2S Homo sapiens cONA clone IMAGE:2428828 Homo sapiens mRNA sequence.

Corynebacterium glutamicum multidrug resistance protein (cmr) gene, complete Corynebacterium cds. glutamicum Arabidopsis thaliana genomic DNA, chromosome 5, TAC clone: K16L22, Arabidopsis thaliana complete sequence.

Arabidopsis thaliana genomic DNA, chromosomeS5, BAC clone:F14A1, complete Arabidopsis thaliana sequence.

Caenorhabditis elegans cosmid F36H9. Caenorhabditis elegans Mycobacterium tuberculosis H37Rv complete genome; segment 51/1 62. Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genome; segment 51/162. Mycobacterium tuberculosis 38,407 35,449 35,449 34,646 35.099 38,562 35,774 41,872 39,139 38,552 34,213 36,461 35.977 38.517 39,173 38,548 46,263 45,053 58,397 52,916 58,809 35,264 29-Apr-99 20-Aug-97 6-Feb-99 20-Aug-97 11-Jun-99 8-Sep-97 18-OCT-1999 9-Apr-97 26-Aug-99 9-Apr-97 20-Nov-99 20-Nov-99 8-Aug-97 17-Jun-98 17-Jun-98 24-Jun-99 17-Jun-98 28-Feb-92 08-DEC-1999 1 5-Jan-99 19-OCT-1998 22-Jan-98 36.218 4-Feb-98 rxa02582 4953 rxa02583 1671 rxa02599 600 Mycobacterium tuberculosis H37Rv complete genome; segment 157/1162.

Mycobacterium tuberculosis H37Rv complete genome; segment 127/1162.

S.erythraea eryA gene for 6-deoxyerythronolyde B synthase 11 Ill.

Streptomyces coelicolor propionyl-CoA carboxylase complex B subunit (pccB) gene, complete cds.

Streptomyces; coelicolor cosmid 1 C2.

Corynebacterium glutamicum dtsRl and dtsR2 genes, complete cds.

Raistonia eutropha mmlH, mmll mmlJ genes.

Mycobacterium tuberculosis Mycobacterium tuberculosis Saccharopolyspora erythraea Streptomyces coelicolor Streptomyces coelicolor Corynebacterium glutamicum Ralstonia eutropha Table 4 (continued) GBEST15:AA508926 422 AA508926 MBAFCW1CO8T3 Brugia malayi adult female cDNA (SAW96MLW-BmnAF) Brugia malayi cDNA clone AFCW1CO8 mRNA sequence.

GBBA1:AEMML 2585 X99639 Ralstonia eutropha mmlH, mmli mmlJ genes.

GB _BA1:SYNPOO 1964 X17439 Synecliocystis ndhC, psbG genes for NDH-C, PSII-G and ORFi 57.

Brugia malayl 43.377 8-Jul-97 rxa02634 1734 Raistonia eutropha .41,148 Synechocystis PCC6803 38,145 GBGSS9:A0101527 184 GBIN1:MNE133341 rxa02638 999 GB -BA2:AE001756 GB-GSS1 2:AQ423878 399 10938 689 A0101527 HS_2265_AlEliMF CIT Approved Human Genomic Sperm Library D Homo H-omo sapiens sapiens genomic clone Plate=2265 CoI-21 Row=I, genomic survey sequence.

AJ13334i Melarthaphe neritoides partial caM gene, exons 1-2. Melartiaphe neritoides AE001756 Thermotoga maritima section 68 of 136 of the complete genome. Thermotoga maritima AQ423878 CITBI-E1-2575E20.TF CITBI-El Homo sapiens genomic clone 2575E20, Homo sapiens 38,798 39,098 40,104 36,451 22-Jan-98 1 0-Feb-99 27-Aug-98 2-Jun-99 2-Jun-99 23-MAR-1999 23-Feb-99 rxa02659 335 GBHTG2:AC006765 274498 GBEST36:A1900317 436 GB.GSS12:AQ342831 683 GBE5T36:A1900856 779 GB N2:CELB0213 39134 GBGSS1:CNSOOPZB 364 rxa02676 1512 GBRO:RNITPR2R rxa02677 882 GBRO:D89728 GBGSS8.AQ062004 10708 5002 362 genomic survey sequence.

AC006765 Caenorhabditis elegans clone Y43H1 1, **SEQUENCING IN PROGRESS-. 7 Caenortiabditis ele unordered pieces.

A1900317 scO4aO2.yl Gm-ci0l2 Glycane max cONA clone GENOME SYSTEMS CLONE Glycine max ID:.Gm-c1 012-1155 5' similar to SW:PRS6 SOLTU P54778 26S PROTEASE REGULATORY SUBUNIT 6B HOMOLOG. mRNA sequence.

AQ342831 RPCI1 1-i 22K1 7.TJ RPCI-1 1 Homo sapiens genomic clone RPCI-1 1-1 22K1 7, Homo sapiens genomic survey sequence.

A1900856 sbg~cllI.yl Gmn-c1 012 Glycine max cONA clone GENOME SYSTEMS CLONE Glycine max ID: Gm-c1012-429 5' similar to SW:PRS6_SOLTU P54778 26S PROTEASE REGULATORY SUBUNIT 613 HOMOLOG. mRNA sequence.

AF039050 Caenortiabditis elegans cosmid B021 3. Caenortiabditis ele AL0851 57 Arabidopsis thaliana genome survey sequence SP6 end of BAC Fl 001 1 of IGF Arabidopsis thaliar library from strain Columbia of Arabidopsis thaliana, genomic survey sequence.

X61677 Rat ITPR2 gene for type 2 inositol triphosphate receptor. Rattus norvegicus 089728 Mus musculus mRNA for LOK, complete cds. Mus musculus A0062004 CIT-HSP-2346014.TR CIT-HSP Homo sapiens genomic clone 2346014, Homo sapiens genomic survey sequence.

A0555818 HS_-5230_-Bi_-G06_-SP6E RPCI-11I Human Male BAC Library Homo sapiens Homo sapiens genomic clone Plate=806 Col=1 1 Row-N, genomic survey sequence.

AJ009736 Drosophila melanogaster Idefix retroelement: gag, pol and env genes, partial. Drosophila melano AC004801 Homo sapiens 12q1 3.1 PAC RPC11-228P16 (Roswell Park Cancer Institute Homo sapiens Human PAC Library) complete sequence.

AC004801 Homo sapiens 12q1 3.1 PAC RPCI1-228P16 (Roswell Park Cancer Institute Homo sapiens Human PAC Library) complete sequence.

AV1 32028 AV1I32028 Mus musculus C57BLI6J 11 -day embryo Mus musculus cDNA clone Mus musculus 2700087F01, mRNA sequence.

A0240654 CIT-HSP-2385D24.TFB.i CIT-HSP Homo sapiens genomic clone 2385024, Homo sapiens genomic s urvey sequence.

A0309500 CIT-HSP-2384D24.TFD CIT-HSP Homo sapiens genomic clone 2384D24, Homo sapiens genomic survey sequence.

34,762 gans gans 39,072 39,063 06-DEC-i1999 35,814 2-Jun-99 0 38,462 28-Jun-9g 37,543 21-OCT-1991 38,829 7-Feb-99 36,565 31-Jul-98 36,534 29-MAY-i1999 36,522 19-Jan-99 39,341 2-Feb-99 37,037 2-Feb-99 41,566 06-DEC-1999 07-MAY-i1999 GBGSS14:A0555818 462 rxa02691 930 GBIN1:DME9736 GBPR4:AC004801 7411 193561 gaster rxa02718 1170 GBPR4:AC004801 193561 GBE5T34:AV132028 258 GBGSSIO:AQ240654 452 GBGSS1i:A0309500 576 43,529 1-Jul-99 40.044 30-Sep-983 38,869 22-DEC-i1998 rxa02749 999 GB-BA2:AF086791 GBBA1:SYCSLRB GB_BA2:AE001 306 rxa02767 906 GB_6A2:AF1 26953 GB..BA1 :SCI5 GBPR3:HS90L6 37867 AF086791 Table 4 (continued) Zymomonas mobilis strain ZM4 clone 67E10 carbamnoylphosphate synthetase Zymomonas mobilis small subunit (carA), carbamaoyiphosphate synthetase large subunit (carB), transcription elongation factor (greA), enolase (eno), pyruvate dehydrogenase alpha subunit (pdhA), pyruvate dehydrogenase beta subunit (pdhB), ribonuclease H homoserine kinase homolog, alcohol dehydrogenase 11 (adhB), and excinuclease ABC subunit A (uvrA) genes, 146271 13316 1638 064000 AE001 306 AF126953 rxa02792 876 GBBA2:AF099015 GB_BA1:ECOUW93 GBHTG3:AC01 1361 GBPR4:AC005998 GB-PR4:AC006008 GBPR3:HS0J73H14 190837 Z97353 5000 AF099015 338534 U14003 188148 AC01 1361 complete cds; and unknown genes.

Synechocystis sp. PCC6803 complete genome, 19/27, 2392729-2538999.

Chlamydia trachomatis section 33 of 87 of the complete genome.

Corynebactenium glutamicum cystathionine gamma-synthase (metB) gene, complete cds.

Streptomyces coelicolor cosmid I5.

Human DNA sequence from clone 901-6 on chromosome 22q1 1.21-11.23.

Contains an RPL1 5 (60S Ribosomal Protein Ll 5) pseudogene, ESTs, STSs and GSSs, complete sequence.

Streptomyces coelicolor strain A3(2) integrase (int), Fe-containing superoxide dismutase 11 (sodF2), Fe uptake system permease (ftrE), and Fe uptake system integral membrane protein (ftrD) genes, complete cds.

Escherichia col K-1 2 chromosomal region from 92.8 to 00. 1 minutes.

Homo sapiens chromosome 5 clone CIT-HSPC_482N19, SEQUENCING IN PROGRESS 69 unordered pieces.

Homo sapiens clone DJ0622E21, complete sequence.

Homo sapiens clone DJ0820A21, complete sequence.

Human DNA sequence from clone 73H14 on chromosome Xq26.3-28, complete Synechocystis sp.

Chlamydia trachomatis Corynebacterium glutamicum Streptomyces coelicolor Homo sapiens 39,024 34,573 38.940 100,000 37,486 34,149 4-Nov-98 1 3-Feb-99 2-Sep-98 10-Sep-99 16-Jun-99 23-Nov-99 1-Jun-99 17-Apr-96 06-OCT-i1999 29-Jul-99 17-Jun-99 23-Nov-99 1 9-Jan-96 1 2-Jun-93 31-Jul-96 27-Aug-99 Streptomyces coelicolor 36,721 rxa02794 1197 96556 57554 95556 3172 894 880 476 AC005998 AC006008 AL080272 Escherichia coli Homo sapiens Homo sapiens Homo sapiens Homo sapiens 38,787 43,577 37,298 36,638 39,726 47,518 44,939 41,554 35,610 rxa02809 375 GBRO:MUSSPCTLT

GB_RO:MUSGRC

GBRO:RNU57062 rxaO281 1 484 GB-GSS6:AQ832862 GBG555A0784593 515 GB_G5513:AQ473140 397 GBEST18:MA696785 316 GBEST18:MA696785 316 sequence.

M22527 Mouse cytotoxic T lymphocyte-specific serine protease CCPII gene, complete Mus musculus M18459 Mouse granzyme C serine esterase mRNA, complete cds. Mus musculus U57062 Rattus norvegicus natural killer cell protease 4 (RNKP-4) mRNA, complete cds. Rattus norvegic A0832862 HS_5261_-A2_-E10OSP6E RPCI-11 Human Male BAC Library Homo sapiens Homo sapiens genomic clone Plate=837 Col=20 Row--l, genomic survey sequence.

A0784593 HS_3248_A2_F02_T7C CIT Approved Human Genomic Sperm Library D Homo Homo sapiens sapiens genomic clone Plate=3248 Col=4 Row=K, genomic survey sequence.

AQ473140 CITBI-E1-2589G6.TF CITBI-El Homo sapiens genomic clone 2589G6, genomic Homo sapiens survey sequence.

AA696785 GM08392.Sprime GM Drosophila mnelanogaster ovary BlueScript Drosophila Drosophila me.

melanogaster cDNA clone GM08392 5prime, mRNA sequence.

AA696785 GM08392.5prime GM Drosophila melanogaster ovary BlueScript Drosophila Drosophila mel melanogaster cDNA clone GM08392 Sprime, mRNA sequence.

:us 38,956 3-Aug-99 34 .76 1 rxa02836 678 anogaster 40,604 anogaster 38,281 23-Apr-99 28-Nov-98 28-Nov-98 8-Sep-97 rxs03212 1452 GB_BA1:CGBETPGEN 2339 X93514 C.glutamicum betP gene.

Corynebacterium 99,931 GBBA1:SC5F2A 40105 AL049587 Streptomyces coelicolor cosmid 5F2A.

glutamicum Streptomyces coelicolor 57,557 A3(2) 24-MAY-1 999 rxs03220 725 GBBA2:AF008220 GBPL1:CKHUP2 GBEST38:AW0481 53 GBPLi :CKHUP2 Table 4 (continued) 220060 AF008220 Bacillus subtilis rrrB-dnaB genomic region. Bacillus subtilis 2353 X66855 C.kessleri HUP2 mRNA. Chiorella kessleri 383 AW048153 UI-M-BH1-alq-h-05-0-UI.sl NIH_-BMAP_-M_-S2 Mus musculus cDNA clone UI-M- Mus musculus BH1-alq-h-05-O-UI mRNA sequence.

2353 X66855 C.kessleri HUP2 mRNA. Chlorella kessleri 40,000 45.328 41,758 38.106 4-Feb-98 17-Feb-97 18-Sep-99 00 17-Feb-97 WO 01/00805 PCT/IB00/00926 -107- Exemplification Example 1: Preparation of total genomic DNA of Corynebacterium glutamicum ATCC 13032 A culture of Corynebacterium glutamicum (ATCC 13032) was grown overnight at 30 0 C with vigorous shaking in BHI medium (Difco). The cells were harvested by centrifugation, the supernatant was discarded and the cells were resuspended in 5 ml buffer-I of the original volume of the culture all indicated volumes have been calculated for 100 ml of culture volume). Composition of buffer-I: 140.34 g/l sucrose, 2.46 g/l MgSO, x 7H,0, 10 ml/I KHPO, solution (100 g/l, adjusted to pH 6.7 with KOH), 50 ml/l M12 concentrate (10 g/l 1 g/1 NaCI, 2 g/l MgSO, x 7H 2 0, 0.2 g/l CaCI,, 0.5 g/l yeast extract (Difco), 10 ml/l trace-elements-mix (200 mg/l FeSO, x H 2 0, 10 mg/l ZnSO, x 7 H 2 0, 3 mg/l MnCI, x 4 H,0, 30 mg/I H,BO, 20 mg/1 CoCl 2 x 6 H 2 0, 1 mg/l NiCI, x 6 H 2 0, 3 mg/l NaMoO x 2 H 2 0, 500 mg/l complexing agent (EDTA or critic acid), 100 ml/l vitamins-mix (0.2 mg/l biotin, 0.2 mg/l folic acid, mg/l p-amino benzoic acid, 20 mg/1 riboflavin, 40 mg/l ca-panthothenate, 140 mg/l nicotinic acid, 40 mg/l pyridoxole hydrochloride, 200 mg/l myo-inositol). Lysozyme was added to the suspension to a final concentration of 2.5 mg/ml. After an approximately 4 h incubation at 37'C, the cell wall was degraded and the resulting protoplasts are harvested by centrifugation. The pellet was washed once with 5 ml buffer-I and once with 5 ml TE-buffer (10 mM Tris-HCI, I mM EDTA, pH The pellet was resuspended in 4 ml TE-buffer and 0.5 ml SDS solution and 0.5 ml NaCI solution (5 M) are added. After adding ofproteinase K to a final concentration of 200 plg/ml, the suspension is incubated for ca.18 h at 37 0 C. The DNA was purified by extraction with phenol, phenol-chloroform-isoamylalcohol and chloroformisoamylalcohol using standard procedures. Then, the DNA was precipitated by adding 1/50 volume of 3 M sodium acetate and 2 volumes of ethanol, followed by a 30 min incubation at -20 0 C and a 30 min centrifugation at 12,000 rpm in a high speed centrifuge using a SS34 rotor (Sorvall). The DNA was dissolved in 1 ml TE-buffer containing pg/ml RNaseA and dialysed at 4°C against 1000 ml TE-buffer for at least 3 hours.

During this time, the buffer was exchanged 3 times. To aliquots of 0.4 ml of the dialysed DNA solution, 0.4 ml of 2 M LiCl and 0.8 ml of ethanol are added. After a -108min incubation at -20 0 C, the DNA was collected by centrifugation (13,000 rpm, Biofuge Fresco, Heraeus, Hanau, Germany). The DNA pellet was dissolved in TE-buffer. DNA prepared by this procedure could be used for all purposes, including southern blotting or construction of genomic libraries.

Example 2: Construction of genomic libraries in Escherichia coli of Corynebacterium glutamicum ATCC13032.

Using DNA prepared as described in Example 1, cosmid and plasmid libraries were constructed according to known and well established methods (see Sambrook, J. et al.

(1989) "Molecular Cloning A Laboratory Manual", Cold Spring Harbor Laboratory Press, or Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley Sons.) .Any plasmid or cosmid could be used. Of particular use were the plasmids pBR322 (Sutcliffe, J.G. (1979) Proc. Natl. Acad Sci. USA, 75:3737-3741); pACYC177 (Change 15 Cohen (1978) J. Bacteriol 134:1141-1156), plasmids of the pBS series (pBSSK+, pBSSK- and others; Stratagene, LaJolla, USA), or cosmids as SuperCosl (Stratagene, LaJolla, USA) or Lorist6 (Gibson, Rosenthal A. and Waterson, R.H. (1987) Gene 53:283-286. Gene libraries specifically for use in C. glutamicum may be constructed using plasmid pSL 109 (Lee, and A. J. Sinskey (1994) J. Microbiol. Biotechnol. 4: 256-263).

S* Example 3: DNA Sequencing and Computational Functional Analysis 9 Genomic libraries as described in Example 2 were used for DNA sequencing according to standard methods, in particular by the chain termination method using ABI377 sequencing machines (see Fleischman, R.D. et al. (1995) "Whole-genome 25 Random Sequencing and Assembly of Haemophilus Influenzae Rd., Science, 269:496- 512). Sequencing primers with the following nucleotide sequences were used: GGAAACAGTATGACCATG-3' (SEQ ID No: 677) or 5'-GTAAAACGACGGCCAGT-3' (SEQ ID No: 678).

Example 4: In vivo Mutagenesis In vivo mutagenesis of Corynebacterium glutamicum can be performed by passage of plasmid (or other vector) DNA through E. coli or other microorganisms Bacillus spp. or yeasts such as Saccharomyces cerevisiae) which are impaired in their capabilities to maintain WO 01/00805 PCT/IB00/00926 -109the integrity of their genetic information. Typical mutator strains have mutations in the genes for the DNA repair system mutHLS, mutD, mutT, etc.; for reference, see Rupp, W.D.

(1996) DNA repair mechanisms, in: Escherichia coli and Salmonella, p. 2277-2294, ASM: Washington.) Such strains are well known to those of ordinary skill in the art. The use of such strains is illustrated, for example, in Greener, A. and Callahan, M. (1994) Strategies 7: 32-34.

Example 5: DNA Transfer Between Escherichia coli and Corynebacterium glutamicum Several Corynebacterium and Brevibacterium species contain endogenous plasmids (as pHM1519 or pBLI) which replicate autonomously (for review see, e.g., Martin, J.F. et al. (1987) Biotechnology, 5:137-146). Shuttle vectors for Escherichia coli and Corynebacterium glutamicum can be readily constructed by using standard vectors for E. coli (Sambrook, J. et al. (1989), "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press or Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley Sons) to which a origin or replication for and a suitable marker from Corynebacterium glutamicum is added. Such origins of replication are preferably taken from endogenous plasmids isolated from Corynebacterium and Brevibacterium species. Of particular use as transformation markers for these species are genes for kanamycin resistance (such as those derived from the Tn5 or Tn903 transposons) or chloramphenicol (Winnacker, E.L. (1987) "From Genes to Clones Introduction to Gene Technology, VCH, Weinheim). There are numerous examples in the literature of the construction of a wide variety of shuttle vectors which replicate in both E.

coli and C. glutamicum, and which can be used for several purposes, including gene overexpression (for reference, see Yoshihama, M. et al. (1985) J. Bacteriol. 162:591-597, Martin J.F. et al. (1987) Biotechnology, 5:137-146 and Eikmanns, B.J. et al. (1991) Gene, 102:93-98).

Using standard methods, it is possible to clone a gene of interest into one of the shuttle vectors described above and to introduce such a hybrid vectors into strains of Corynebacterium glutamicum. Transformation of C. glutamicum can be achieved by protoplast transformation (Kastsumata, R. et al. (1984) J. Bacteriol. 159306-311), electroporation (Liebl, E. et al. (1989) FEMS Microbiol. Letters, 53:399-303) and in cases where special vectors are used, also by conjugation (as described e.g. in Schtifer, WO 01/00805 PCT/IB00/00926 -110- A et al. (1990) J Bacteriol. 172:1663-1666). It is also possible to transfer the shuttle vectors for C. glutamicum to E. coli by preparing plasmid DNA from C. glutamicum (using standard methods well-known in the art) and transforming it into E. coli. This transformation step can be performed using standard methods, but it is advantageous to use an Mcr-deficient E. coli strain, such as NM522 (Gough Murray (1983) J. Mol.

Biol. 166:1-19).

Genes may be overexpressed in C. glutamicum strains using plasmids which comprise pCGI Patent No. 4,617,267) or fragments thereof, and optionally the gene for kanamycin resistance from TN903 (Grindley, N.D. and Joyce, C.M. (1980) Proc. Natl. Acad. Sci. USA 77(12): 7176-7180). In addition, genes may be overexpressed in C. glutamicum strains using plasmid pSL109 (Lee, and A. J.

Sinskey (1994) J. Microbiol. Biotechnol. 4: 256-263).

Aside from the use of replicative plasmids, gene overexpression can also be achieved by integration into the genome. Genomic integration in C. glutamicum or other Corynebacterium or Brevibacterium species may be accomplished by well-known methods, such as homologous recombination with genomic region(s), restriction endonuclease mediated integration (REMI) (see, DE Patent 19823834), or through the use of transposons. It is also possible to modulate the activity of a gene of interest by modifying the regulatory regions a promoter, a repressor, and/or an enhancer) by sequence modification, insertion, or deletion using site-directed methods (such as homologous recombination) or methods based on random events (such as transposon mutagenesis or REMI). Nucleic acid sequences which function as transcriptional terminators may also be inserted 3' to the coding region of one or more genes of the invention; such terminators are well-known in the art and are described, for example, in Winnacker, E.L. (1987) From Genes to Clones Introduction to Gene Technology. VCH: Weinheim.

Example 6: Assessment of the Expression of the Mutant Protein Observations of the activity of a mutated protein in a transformed host cell rely on the fact that the mutant protein is expressed in a similar fashion and in a similar quantity to that of the wild-type protein. A useful method to ascertain the level of transcription of the mutant gene (an indicator of the amount of mRNA available for translation to the gene WO 01/00805 PCT/IB00/00926 -111 product) is to perform a Northern blot (for reference see, for example, Ausubel et al.

(1988) Current Protocols in Molecular Biology, Wiley: New York), in which a primer designed to bind to the gene of interest is labeled with a detectable tag (usually radioactive or chemiluminescent), such that when the total RNA of a culture of the organism is extracted, run on gel, transferred to a stable matrix and incubated with this probe, the binding and quantity of binding of the probe indicates the presence and also the quantity of mRNA for this gene. This information is evidence of the degree of transcription of the mutant gene. Total cellular RNA can be prepared from Corynebacterium glutamicum by several methods, all well-known in the art, such as that described in Bormann, E.R. et al.

(1992) Mol. Microbiol. 6: 317-326.

To assess the presence or relative quantity of protein translated from this mRNA, standard techniques, such as a Western blot, may be employed (see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: New York). In this process, total cellular proteins are extracted, separated by gel electrophoresis, transferred to a matrix such as nitrocellulose, and incubated with a probe, such as an antibody, which specifically binds to the desired protein. This probe is generally tagged with a chemiluminescent or colorimetric label which may be readily detected. The presence and quantity of label observed indicates the presence and quantity of the desired mutant protein present in the cell.

Example 7: Growth of Genetically Modified Corynebacterium glutamicum Media and Culture Conditions Genetically modified Corynebacteria are cultured in synthetic or natural growth media. A number of different growth media for Corynebacteria are both well-known and readily available (Lieb et al. (1989) Appl. Microbiol. Biotechnol., 32:205-210; von der Osten et al. (1998) Biotechnology Letters, 11:11-16; Patent DE 4,120,867; Liebl (1992) "The Genus Corynebacterium, in: The Procaryotes, Volume II, Balows, A. et al., eds.

Springer-Verlag). These media consist of one or more carbon sources, nitrogen sources, inorganic salts, vitamins and trace elements. Preferred carbon sources are sugars, such as mono-, di-, or polysaccharides. For example, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch or cellulose serve as very good carbon sources. It is also possible to supply sugar to the media via complex WO 01/00805 PCT/IB00/00926 -112compounds such as molasses or other by-products from sugar refinement. It can also be advantageous to supply mixtures of different carbon sources. Other possible carbon sources are alcohols and organic acids, such as methanol, ethanol, acetic acid or lactic acid. Nitrogen sources are usually organic or inorganic nitrogen compounds, or materials which contain these compounds. Exemplary nitrogen sources include ammonia gas or ammonia salts, such as NH,CI or NI-IOH, nitrates, urea, amino acids or complex nitrogen sources like corn steep liquor, soy bean flour, soy bean protein, yeast extract, meat extract and others.

Inorganic salt compounds which may be included in the media include the chloride-, phosphorous- or sulfate- salts of calcium, magnesium, sodium, cobalt, molybdenum, potassium, manganese, zinc, copper and iron. Chelating compounds can be added to the medium to keep the metal ions in solution. Particularly useful chelating compounds include dihydroxyphenols, like catechol or protocatechuate, or organic acids, such as citric acid. It is typical for the media to also contain other growth factors, such as vitamins or growth promoters, examples of which include biotin, riboflavin, thiamin, folic acid, nicotinic acid, pantothenate and pyridoxin. Growth factors and salts frequently originate from complex media components such as yeast extract, molasses, corn steep liquor and others. The exact composition of the media compounds depends strongly on the immediate experiment and is individually decided for each specific case. Information about media optimization is available in the textbook "Applied Microbiol. Physiology, A Practical Approach (eds. P.M. Rhodes, P.F. Stanbury, IRL Press (1997) pp. 53-73, ISBN 0 19 963577 It is also possible to select growth media from commercial suppliers, like standard 1 (Merck) or BHI (grain heart infusion, DIFCO) or others.

All medium components are sterilized, either by heat (20 minutes at 1.5 bar and 121 C) or by sterile filtration. The components can either be sterilized together or, if necessary, separately. All media components can be present at the beginning of growth, or they can optionally be added continuously or batchwise.

Culture conditions are defined separately for each experiment. The temperature should be in a range between 15'C and 45C. The temperature can be kept constant or can be altered during the experiment. The pH of the medium should be in the range of 5 to preferably around 7.0, and can be maintained by the addition of buffers to the media.

An exemplary buffer for this purpose is a potassium phosphate buffer. Synthetic buffers WO 01/00805 PCT/IB00/00926 113such as MOPS, HEPES, ACES and others can alternatively or simultaneously be used. It is also possible to maintain a constant culture pH through the addition of NaOH or NH,OH during growth. If complex medium components such as yeast extract are utilized, the necessity for additional buffers may be reduced, due to the fact that many complex compounds have high buffer capacities. If a fermentor is utilized for culturing the microorganisms, the pH can also be controlled using gaseous ammonia.

The incubation time is usually in a range from several hours to several days. This time is selected in order to permit the maximal amount of product to accumulate in the broth. The disclosed growth experiments can be carried out in a variety of vessels, such as microtiter plates, glass tubes, glass flasks or glass or metal fermentors of different sizes.

For screening a large number of clones, the microorganisms should be cultured in microtiter plates, glass tubes or shake flasks, either with or without baffles. Preferably 100 ml shake flasks are used, filled with 10% (by volume) of the required growth medium. The flasks should be shaken on a rotary shaker (amplitude 25 mm) using a speed-range of 100 300 rpm. Evaporation losses can be diminished by the maintenance of a humid atmosphere; alternatively, a mathematical correction for evaporation losses should be performed.

If genetically modified clones are tested, an unmodified control clone or a control clone containing the basic plasmid without any insert should also be tested. The medium is inoculated to an OD 6 0 o of 0.5 1.5 using cells grown on agar plates, such as CM plates g/1 glucose, 2,5 g/l NaCI, 2 g/1 urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l NaCI, 2 g/1 urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l agar, pH 6.8 with 2M NaOH) that had been incubated at 30'C. Inoculation of the media is accomplished by either introduction of a saline suspension of C. glutamicum cells from CM plates or addition of a liquid preculture of this bacterium.

Example 8 -In vitro Analysis of the Function of Mutant Proteins The determination of activities and kinetic parameters of enzymes is well established in the art. Experiments to determine the activity of any given altered enzyme must be tailored to the specific activity of the wild-type enzyme, which is well within the ability of one of ordinary skill in the art. Overviews about enzymes in general, as well as specific details concerning structure, kinetics, principles, methods, WO 01/00805 PCT/IB00/00926 -114applications and examples for the determination of many enzyme activities may be found, for example, in the following references: Dixon, and Webb, (1979) Enzymes. Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism.

Freeman: New York; Walsh, (1979) Enzymatic Reaction Mechanisms. Freeman: San Francisco; Price, Stevens, L. (1982) Fundamentals of Enzymology. Oxford Univ.

Press: Oxford; Boyer, ed. (1983) The Enzymes, 3 r d ed. Academic Press: New York; Bisswanger, (1994) Enzymkinetik, 2 nd ed. VCH: Weinheim (ISBN 3527300325); Bergmeyer, Bergmeyer, GraBl, eds. (1983-1986) Methods of Enzymatic Analysis, 3 r d ed., vol. I-XII, Verlag Chemie: Weinheim; and Ullmann's Encyclopedia of Industrial Chemistry (1987) vol. A9, "Enzymes". VCH: Weinheim, p.

352-363.

The activity of proteins which bind to DNA can be measured by several wellestablished methods, such as DNA band-shift assays (also called gel retardation assays).

The effect of such proteins on the expression of other molecules can be measured using reporter gene assays (such as that described in Kolmar, H. et al. (1995) EMBO J. 14: 3895-3904 and references cited therein). Reporter gene test systems are well known and established for applications in both pro- and eukaryotic cells, using enzymes such as beta-galactosidase, green fluorescent protein, and several others.

The determination of activity of membrane-transport proteins can be performed according to techniques such as those described in Gennis, R.B. (1989) "Pores, Channels and Transporters", in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p. 85-137; 199-234; and 270-322.

Example 9: Analysis of Impact of Mutant Protein on the Production of the Desired Product The effect of the genetic modification in C. glutamicum on production of a desired compound (such as an amino acid) can be assessed by growing the modified microorganism under suitable conditions (such as those described above) and analyzing the medium and/or the cellular component for increased production of the desired product an amino acid). Such analysis techniques are well known to one of ordinary skill in the art, and include spectroscopy, thin layer chromatography, staining methods of various kinds, enzymatic and microbiological methods, and analytical WO 01/00805 PCT/IB00/00926 -115chromatography such as high performance liquid chromatography (see, for example, Ullman, Encyclopedia of Industrial Chemistry, vol. A2, p. 89-90 and p. 443-613, VCH: Weinheim (1985); Fallon, A. et al., (1987) "Applications of HPLC in Biochemistry" in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17; Rehm et al.

(1993) Biotechnology, vol. 3, Chapter III: "Product recovery and purification", page 469-714, VCH: Weinheim; Belter, P.A. et al. (1988) Bioseparations: downstream processing for biotechnology, John Wiley and Sons; Kennedy, J.F. and Cabral, J.M.S.

(1992) Recovery processes for biological materials, John Wiley and Sons; Shaeiwitz, J.A. and Henry, J.D. (1988) Biochemical separations, in: Ulmann's Encyclopedia of Industrial Chemistry, vol. B3, Chapter 11, page 1-27, VCH: Weinheim; and Dechow, F.J. (1989) Separation and purification techniques in biotechnology, Noyes Publications.) In addition to the measurement of the final product of fermentation, it is also possible to analyze other components of the metabolic pathways utilized for the production of the desired compound, such as intermediates and side-products, to determine the overall efficiency of production of the compound. Analysis methods include measurements of nutrient levels in the medium sugars, hydrocarbons, nitrogen sources, phosphate, and other ions), measurements of biomass composition and growth, analysis of the production of common metabolites of biosynthetic pathways, and measurement of gasses produced during fermentation. Standard methods for these measurements are outlined in Applied Microbial Physiology, A Practical Approach, P.M. Rhodes and P.F. Stanbury, eds., IRL Press, p. 103-129; 131-163; and 165-192 (ISBN: 0199635773) and references cited therein.

Example 10: Purification of the Desired Product from C. glutamicum Culture Recovery of the desired product from the C. glutamicum cells or supernatant of the above-described culture can be performed by various methods well known in the art.

If the desired product is not secreted from the cells, the cells can be harvested from the culture by low-speed centrifugation, the cells can be lysed by standard techniques, such as mechanical force or sonication. The cellular debris is removed by centrifugation, and the supernatant fraction containing the soluble proteins is retained for further purification of the desired compound. If the product is secreted from the C. glutamicum WO 01/00805 PCT/IB00/00926 -116cells, then the cells are removed from the culture by low-speed centrifugation, and the supemate fraction is retained for further purification.

The supernatant fraction from either purification method is subjected to chromatography with a suitable resin, in which the desired molecule is either retained on a chromatography resin while many of the impurities in the sample are not, or where the impurities are retained by the resin while the sample is not. Such chromatography steps may be repeated as necessary, using the same or different chromatography resins. One of ordinary skill in the art would be well-versed in the selection of appropriate chromatography resins and in their most efficacious application for a particular molecule to be purified. The purified product may be concentrated by filtration or ultrafiltration, and stored at a temperature at which the stability of the product is maximized.

There are a wide array of purification methods known to the art and the preceding method of purification is not meant to be limiting. Such purification techniques are described, for example, in Bailey, J.E. Ollis, D.F. Biochemical Engineering Fundamentals, McGraw-Hill: New York (1986).

The identity and purity of the isolated compounds may be assessed by techniques standard in the art. These include high-performance liquid chromatography (HPLC), spectroscopic methods, staining methods, thin layer chromatography, NIRS, enzymatic assay, or microbiologically. Such analysis methods are reviewed in: Patek et al. (1994) Appl. Environ. Microbiol. 60: 133-140; Malakhova et al. (1996) Biotekhnologiya 11: 27- 32; and Schmidt et al. (1998) Bioprocess Engineer. 19: 67-70. Ulmann's Encyclopedia of Industrial Chemistry, (1996) vol. A27, VCH: Weinheim, p. 89-90, p. 521-540, p. 540- 547, p. 559-566, 575-581 and p. 581-587; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons; Fallon, A. et al.

(1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17.

Example 11: Analysis of the Gene Sequences of the Invention The comparison of sequences and determination of percent homology between two sequences are art-known techniques, and can be accomplished using a mathematical algorithm, such as the algorithm of Karlin and Altschul (1990) Proc. Nail. Acad. Sci.

USA 87:2264-68, modified as in Karlin and Altschul (1993) Proc. Natl. Acad. Sci. USA WO 01/00805 PCTfIB00/00926 -117- 90:5873-77. Such an algorithm is incorporated into the NBLAST and XBLAST programs (version 2.0) of Altschul, et al. (1990) J. Mol. Biol. 215:403-10. BLAST nucleotide searches can be performed with the NBLAST program, score 100, wordlength 12 to obtain nucleotide sequences homologous to MCT nucleic acid molecules of the invention. BLAST protein searches can be performed with the XBLAST program, score 50, wordlength 3 to obtain amino acid sequences homologous to MCT protein molecules of the invention. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., (1997) Nucleic Acids Res. 25(17):3389-3402. When utilizing BLAST and Gapped BLAST programs, one of ordinary skill in the art will know how to optimize the parameters of the program XBLAST and NBLAST) for the specific sequence being analyzed.

Another example of a mathematical algorithm utilized for the comparison of sequences is the algorithm of Meyers and Miller ((1988) Comput. Appl. Biosci. 4: 11- 17). Such an algorithm is incorporated into the ALIGN program (version 2.0) which is part of the GCG sequence alignment software package. When utilizing the ALIGN program for comparing amino acid sequences, a PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used. Additional algorithms for sequence analysis are known in the art, and include ADVANCE and ADAM. described in Torelli and Robotti (1994) Comput. Appl. Biosci. 10:3-5; and FASTA, described in Pearson and Lipman (1988) P.N.A.S. 85:2444-8.

The percent homology between two amino acid sequences can also be accomplished using the GAP program in the GCG software package (available at http://www.gcg.com), using either a Blosum 62 matrix or a PAM250 matrix, and a gap weight of 12, 10, 8, 6, or 4 and a length weight of 2, 3, or 4. The percent homology between two nucleic acid sequences can be accomplished using the GAP program in the GCG software package, using standard parameters, such as a gap weight of 50 and a length weight of 3.

A comparative analysis of the gene sequences of the invention with those present in Genbank has been performed using techniques known in the art (see, Bexevanis and Ouellette, eds. (1998) Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins. John Wiley and Sons: New York). The gene sequences of the invention WO 01100805 PCT/IBOO/00926 -118were compared to genes present in Genbank in a three-step process. In a first step, a BLASTN analysis a local alignment analysis) was performed for each of the sequences of the invention against the nucleotide sequences present in Genbank, and the top 500 hits were retained for further analysis. A subsequent FASTA search a combined local and global alignment analysis, in which limited regions of the sequences are aligned) was performed on these 500 hits. Each gene sequence of the invention was subsequently globally aligned to each of the top three FASTA hits, using the GAP program in the GCG software package (using standard parameters). In order to obtain correct results, the length of the sequences extracted from Genbank were adjusted to the length of the query sequences by methods well-known in the art. The results of this analysis are set forth in Table 4. The resulting data is identical to that which would have been obtained had a GAP (global) analysis alone been performed on each of the genes of the invention in comparison with each of the references in Genbank, but required significantly reduced computational time as compared to such a database-wide GAP (global) analysis. Sequences of the invention for which no alignments above the cutoff values were obtained are indicated on Table 4 by the absence of alignment information.

It will further be understood by one of ordinary skill in the art that the GAP alignment homology percentages set forth in Table 4 under the heading homology (GAP)" are listed in the European numerical format, wherein a represents a decimal point. For example, a value of "40,345" in this column represents "40.345%".

Example 12: Construction and Operation of DNA Microarrays The sequences of the invention may additionally be used in the construction and application of DNA microarrays (the design, methodology, and uses of DNA arrays are well known in the art, and are described, for example, in Schena, M. et al. (1995) Science 270: 467-470; Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359-1367; DeSaizieu, A. et al. (1998) Nature Biotechnology 16: 45-48; and DeRisi, J.L. et al.

(1997) Science 278: 680-686).

DNA microarrays are solid or flexible supports consisting of nitrocellulose, nylon, glass, silicone, or other materials. Nucleic acid molecules may be attached to the surface in an ordered manner. After appropriate labeling, other nucleic acids or nucleic acid mixtures can be hybridized to the immobilized nucleic acid molecules, and the label WO 01/00805 PCT/IB00/00926 -119may be used to monitor and measure the individual signal intensities of the hybridized molecules at defined regions. This methodology allows the simultaneous quantification of the relative or absolute amount of all or selected nucleic acids in the applied nucleic acid sample or mixture. DNA microarrays, therefore, permit an analysis of the expression of multiple (as many as 6800 or more) nucleic acids in parallel (see, e.g., Schena, M. (1996) BioEssays 18(5): 427-431).

The sequences of the invention may be used to design oligonucleotide primers which are able to amplify defined regions of one or more C glutamicum genes by a nucleic acid amplification reaction such as the polymerase chain reaction. The choice and design of the 5' or 3' oligonucleotide primers or of appropriate linkers allows the covalent attachment of the resulting PCR products to the surface of a support medium described above (and also described, for example, Schena, M. et al. (1995) Science 270: 467-470).

Nucleic acid microarrays may also be constructed by in situ oligonucleotide synthesis as described by Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359- 1367. By photolithographic methods, precisely defined regions of the matrix are exposed to light. Protective groups which are photolabile are thereby activated and undergo nucleotide addition, whereas regions that are masked from light do not undergo any modification. Subsequent cycles of protection and light activation permit the synthesis of different oligonucleotides at defined positions. Small, defined regions of the genes of the invention may be synthesized on microarrays by solid phase oligonucleotide synthesis.

The nucleic acid molecules of the invention present in a sample or mixture of nucleotides may be hybridized to the microarrays. These nucleic acid molecules can be labeled according to standard methods. In brief, nucleic acid molecules mRNA molecules or DNA molecules) are labeled by the incorporation of isotopically or fluorescently labeled nucleotides, during reverse transcription or DNA synthesis.

Hybridization of labeled nucleic acids to microarrays is described in Schena, M. et al. (1995) supra; Wodicka, L. et al. (1997), supra; and DeSaizieu A. et al. (1998), supra). The detection and quantification of the hybridized molecule are tailored to the specific incorporated label. Radioactive labels can be detected, for example, as WO 01/00805 PCT/IB00/00926 -120described in Schena, M. et al. (1995) supra) and fluorescent labels may be detected, for example, by the method of Shalon et al. (1996) Genome Research 6: 639-645).

The application of the sequences of the invention to DNA microarray technology, as described above, permits comparative analyses of different strains of C.

glutamicum or other Corynebacteria. For example, studies of inter-strain variations based on individual transcript profiles and the identification of genes that are important for specific and/or desired strain properties such as pathogenicity, productivity and stress tolerance are facilitated by nucleic acid array methodologies. Also, comparisons of the profile of expression of genes of the invention during the course of a fermentation reaction are possible using nucleic acid array technology.

Example 13: Analysis of the Dynamics of Cellular Protein Populations (Proteomics) The genes, compositions, and methods of the invention may be applied to study the interactions and dynamics of populations of proteins, termed 'proteomics'. Protein populations of interest include, but are not limited to, the total protein population of C.

glutamicum in comparison with the protein populations of other organisms), those proteins which are active under specific environmental or metabolic conditions during fermentation, at high or low temperature, or at high or low pH), or those proteins which are active during specific phases of growth and development.

Protein populations can be analyzed by various well-known techniques, such as gel electrophoresis. Cellular proteins may be obtained, for example, by lysis or extraction, and may be separated from one another using a variety of electrophoretic techniques. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins largely on the basis of their molecular weight. Isoelectric focusing polyacrylamide gel electrophoresis (IEF-PAGE) separates proteins by their isoelectric point (which reflects not only the amino acid sequence but also posttranslational modifications of the protein). Another, more preferred method of protein analysis is the consecutive combination of both IEF-PAGE and SDS-PAGE, known as 2-D-gel electrophoresis (described, for example, in Hermann et al. (1998) Electrophoresis 19: 3217-3221; Fountoulakis et al. (1998) Electrophoresis 19: 1193-1202; Langen et al.

(1997) Electrophoresis 18: 1184-1192; Antelmann et al. (1997) Electrophoresis 18: WO 01/00805 PCT/IB00/00926 121 1451-1463). Other separation techniques may also be utilized for protein separation, such as capillary gel electrophoresis; such techniques are well known in the art.

Proteins separated by these methodologies can be visualized by standard techniques, such as by staining or labeling. Suitable stains are known in the art, and include Coomassie Brilliant Blue, silver stain, or fluorescent dyes such as Sypro Ruby (Molecular Probes). The inclusion of radioactively labeled amino acids or other protein precursors "S-methionine, "S-cysteine, 1 4 C-labelled amino acids, 1N-amino acids, 5

NO

3 or "'NH4 or 13 C-labelled amino acids) in the medium of C. glutamicum permits the labeling of proteins from these cells prior to their separation. Similarly, fluorescent labels may be employed. These labeled proteins can be extracted, isolated and separated according to the previously described techniques.

Proteins visualized by these techniques can be further analyzed by measuring the amount of dye or label used. The amount of a given protein can be determined quantitatively using, for example, optical methods and can be compared to the amount of other proteins in the same gel or in other gels. Comparisons of proteins on gels can be made, for example, by optical comparison, by spectroscopy, by image scanning and analysis of gels, or through the use of photographic films and screens. Such techniques are well-known in the art.

To determine the identity of any given protein, direct sequencing or other standard techniques may be employed. For example, N- and/or C-terminal amino acid sequencing (such as Edman degradation) may be used, as may mass spectrometry (in particular MALDI or ESI techniques (see, Langen et al. (1997) Electrophoresis 18: 1184-1192)). The protein sequences provided herein can be used for the identification of C. glutamicum proteins by these techniques.

The information obtained by these methods can be used to compare patterns of protein presence, activity, or modification between different samples from various biological conditions different organisms, time points of fermentation, media conditions, or different biotopes, among others). Data obtained from such experiments alone, or in combination with other techniques, can be used for various applications, such as to compare the behavior of various organisms in a given metabolic) situation, to increase the productivity of strains which produce fine chemicals or to increase the efficiency of the production of fine chemicals.

06/10 '05 THU 10:55 FAX 61 2 9888 7600 WATERMARK J010 122

EQUIVALENTS

Those of ordinary skill in the art will recognize, or will be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

Comprises/comprising and grammatical variations thereof when used in this specification are to be taken to specify the presence of stated features, integers, steps or components or groups thereof, but do not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof.

e* **eg *e COMS ID No: SBMI-01674575 Received by IP Australia: Time 11:03 Date 2005-10-06 EDITORIAL NOTE APPLICATION NUMBER 54216-00 The following Sequence Listing pages 1 to 990 are part of the description. The claims pages follow on pages "123" to "127".

wo 01/00805 WO 0100805PCT/IBOU/00926 SEQUENCE LISTING <110> BASF Aktiengesellschaft <120> CORYNEBACTERIUM GLUTAMICUM GENES ENCODING PROTEINS INVOLVED IN MEMBRANE SYNTHESIS AND MEMBRANE TRANS PORT <130> BGI-125CPPC <140> <141> <160> 676 <210> <211> <212> <213> <220> <221> <222> <223> 578

DNA

Corynebacterium giutamicum

CDS

RXNO03097 <400> 1 att tca Ile Ser 1 gcc agt gca Ala Ser Ala 5 gqt gct ata ggt Gly Ala Ile Gly tta att tta gaa Leu Ile Leu Giu tat att Tyr Ile ttt aaa aag Phe Lys Lys gga tta gtt Gly Leu Val aca agt tta ctt Thr Ser Leu Leu gga Gly ctt tta ctc qgt Leu Leu Leu Gly gca tta gca Ala Leu Ala tat ctt agt Tyr Leu Ser gtt att act cct Val Ilie Thr Pro gca gga tat gta Ala Gly Tyr Val gca aca Ala Thr ata atg gct tta Ile Met Ala Leu gga qqt atc tgt tgt Gly Gly Ile Cys Cys tat att gtc att Tyr Ile Val Ile aat Asn tac atc aag gta Tyr Ile Lys Val aaa Lys 70 cta aaa tat cat Leu Lys Tyr His ga t Asp gca tta gat gca Ala Leu Asp Ala ggt att cat ggt Gly Ilie His Gly ggt ggt att att Gly Gly Ile Ilie qct gtt tta aca Ala Val Leu Thr gca gtt Ala Val ttc caa agt Phe Gin Ser aaa gcc aat cct Lys Ala Asn Pro att gag aat ggc Ile Giu Asn Gly ttt att tat Phe Ile Tyr 110 gta aca gca Val Thr Ala act ggt gac ata cat att ata Thr Gly Asp Ilie His Ilie Ile gta caa ata tta Val Gin Ilie Leu gtt gta Val Val 130 att ttt agt atc Ile Phe Ser Ile gtc Val 135 atg acg ttt att Met Thr Phe Ile att Ile 140 gcg aaa gta att Ala Lys Val Ile 384 432 480 aaa Lys 145 tta att aca cca Leu Ile Thr Pro tct gtt acg gaa Ser Val Thr Giu gaa acg aat ata Giu Thr Asn Ile gga Gi y 160 WO 01/00805 WO 0100805PCTIBOOIOO926 tta gac aag att gtt cac ggt gaa cat gct tac ttt gaa ggt gag cta Leu Asp Lys Ile Val His Gly Glu His Ala Tyr Phe Giu Gly Glu Leu 165 170 175 aat aga ttc aat aaa cat att cga tat tagaatatat ttacatagaa Asn Arg Phe Asn Lys His Ile Arg Tyr 180 185 tat <210> 2 <211> 185 <212> PRT <213> Corynebacterium giutarnicum <400> 2 Ile Ser Ala Ser Ala Gly Ala Ile Gly Lys Leu Th r T yr Ile Gin Gly Val1 130 Le u Asp Arg Lys Val1 Ilie Ile His Ser Asp 115 Ile Ile Lys Phe Th r Ile Ala ValI Val1 Lys His Ser Pro Val 165 Lys Se r Th r Le u Lys Gly Ala Ilie Ile Le u 150 His His Le u Pro Ile 55 Le u Gly As n Ilie Val1 135 Se r Gly Ile Leu Ala Gly Lys Ile Pro Le u 120 Met Val1 Glu Arg Gly Ala Gi y T yr Ile Asp 105 Val1 Thr Th r His T yr 185 Trp 10 Leu Gly Ile H is Gly 90 Ile Gin Phe Giu Al a 170 Le u Leu Tyr Cys Asp 75 Ala Giu Ile Ile Gin 155 Ty r Ile Le u Val C ys Al a Val Asn Leu Ile 140 Glu Phe Glu Al a Tyr Ile Asp Thr Phe 110 Val1 Lys As n Gi y Tyr Leu Leu Val1 Ala Ala Ile Th r Val1 Ile Giu 175 Ile Ala Se r Ile Phe Val1 T yr Ala Ile Gly 160 Le u <210> 3 <211> 373 <212> DNA <213> Corynebacterium glutamicum <2 WO 01/00805 <221> CDS <222> (373) <223> RXA02099 <400> 3 tagttagagc tggttcaagg gqtqtcaatc ccaaaaggca gcttgacaaa acttcaacgt caaaggaggt catccacgct PCT/1B00100926 att Ile ctc Leu t ct Se r t tq Le u tat Tyr ttc Phe gt c ValI cta Le u a ag Lys gtt Vai caa Gin aaa Lys tc Ser atg Met tcc Se r act Thr t ca Ser gat Asp gqC Gly aca Thr qtq Val1 gtt Val1 atc Ile 75 tcc Ser ctccttgaac tcatgaaaaa atg gqt gca gat caa Met Gly Ala Asp Gin 1 ctq atg tcc gcq tcq Leu Met Ser Ala Ser ttc tat ggc qgc atq Phe Tyr Gly Gly Met.

atg tcc ttt gga gca Met Ser Phe Gly Ala qga tgq tcg atq tct Gly Trp Ser Met Ser aac cct ttt gag ttc Asn Pro Phe Giu Phe <210> 4 <211> 91 <212> PRT <213> Corynebacterium glutamicum <400> 4 Met Gly Ala Asp Gin Ile Ala Ala 1 5 Leu Met Ser Ala Ser Leu Val Leu Phe Tyr Gly Gly Met Ser Arq Gin 40 Met Ser Phe Gly Ala Leu Gly Val 55 Gly Trp Ser Met Ser Tyr Gly Thr 70 Asn Pro Phe Glu Phe Phe Gly Leu Gly Asn Ser Ala Trp Thr Pro Ala Leu Ala Val Leu Asn Met Met Val Ile Tyr Leu Leu Ile Ala Gly Ile Phe Se r WO 01/00805 <210> <211> 879 <212> DNA <213> Corynebacterium giutamioum <220> <221> CDS <222> (101)..(856) <223> RXA00104 <400> tgoaaagaca toooggaaco aotccqotac ttgaggtcgg PCT/IBOO/00926 aatcgggaaa qaatgctttt agocatgoot taatgtaacc aaaoatctag aattgagaao atq act got caq att Met Thr Ala Gin Ile gat Asp ctc Leu gat Asp cag Gin cca Pro 9gC Gly goa Ala g ac Asp cot Pro got Al a 150 gat Asp aaa Lys qco Ala oac His gao Asp acc Th r ct g Le u ctt Le u tac Ty r 135 ota Le u tog Ser ggt Gly ggo Gly og 0 Arg ogc Arg aaa Lys qta Val 9gc Gly 120 too Se r tot Se r cat His gtt ValI qca Aia gga Gly 60 gao Asp aco Thr gtt Val1 cac His gcc Ala 140 cag Gin cgt Arg ggq Giy caa Gin 45 ttc Phe cgc Arg qg Giy ccc Pro tcc Se r 125 atc Ile ctc Le u cto Leu gtg Val1 agt Se r ctg Le u gtq ValI cg c Arg acc Th r 110 qot Al a too Se r ggc Gly gcc Ala tta Le u t q Trp tot Se r tgg Trp cag Gin cac His ga t Asp cac His ttt Phe ca a Gin aqa Arg att Ile gaa Glu atc Ile 80 gac Asp qcc Ala gc Ala aac As n gaa Glu 160 cto Le u 1 qgc Gly qgt Gly got Ala gaa Glu ato Ile t gg Trp got Ala ogo Arg og 0 Arq 145 aoo Th r ggt Gly aoo Thr cgg Arg oga Arg gcc Al a qa t Asp g ca Ala gt t Val goo Ala 130 ca Pro aag Lys gao Asp gga Gly aat As n g tg Val1 goo Ala 0CC Pro gta Val1 Gly 115 gtg Val ooa Pro gc Ala tao Tyr ga a Gi u ot 0 Le u ttg Leu gao Asp oto Leu cac His 100 oto Leu aot Th r aag Lys ott Le u gao Asp 180 ato Ile ggt Gly gag Giu aao Asn gao Asp atc Ile 000 Pro gq 0 Gly gtt Val1 gga Gly 165 goo Ala 115 163 211 259 307 355 403 451 499 547 595 643 too goa gqo Ser Ala Gly got aaa goa atg oac gtt oto Ala Lys 170 Ala Met His Val Leu 175 WO 01/00805 atc cac qc Ile His Al 18 gtc tgc aa Val Cys Ly 200 ctg acc ta Leu Thr Ty 215 cgc cct ga Arg Pro G1 gaq gag aa Giu Giu As tac gag tgg qat tcc gca gca Tyr Glu Trp Asp Ser Ala Ala 190 195 ttg cac tgc tcc agg ctc gac Leu His Cys Ser Arg Leu Asp 205 210 gac acc tac atg cca gac atc Asp Thr Tyr Met Pro Asp Ile 225 gaa ctt ctc. gag atg tgc gcg Glu Leu Leu Giu Met Cys Ala 240 taacqctgtt atgatgacgg cat cca Pro gg t Gly ttg Le u aag Lys PCTIBOO/00926 gtc 691 ValI tcc 739 Se r atc 787 Ile ttc 835 Phe 245 (210> 6 (211> 252 <212> PRT (213> Corynebacteriun giutamicum (400> 6 Met Thr Ala Gin Ile Asp Asp Ser 1 Gly Gly Ala Giu Ile Trp Ala Arg Arg 145 Th r Th r Arg Arg Al a Asp Ala Vali Al a 130 Pro Lys 5 Ile Gly Glu As n Asp Ile Pro Gl y ValI Gly Leu Asp Gin Pro 70 Gly Al a Asp Pro Ala 150 Se r Lys Aila His 55 Asp Th r Le u Le u Tyr 135 Le u Ala Gly Gly Arg Arg L ys Val Gly 120 Se r Ser Gly Ile Leu 10 Val Arg Asp Giu Pro Asn Leu Ser Glu Phe 90 Giu Asn 105 Val Vai Lys Vai Cys Ala Ala Lys 170 Thr As n Le u Asp Lys 75 Al a Gly Phe Ile Glu 155 Ala Gi y His Val Ala Gly Asp Thr Vali His Ala 140 Gin Met Gin Arg Gly Gln Phe Arg Giy Pro Ser 125 Ile Le u His Tyr Leu ValI Se r Le u Val Arg Thr 110 Ala Se r Gl y Val G iu Ala Le u T rp Ser Trp Gin His Asp His Phe Le u 175 Trp Gin Arg Ile Giu Ile Asp Ala Ala Asn Giu 160 Le u Asp 165 Giy Asp Tyr Asp Ala Tyr Ile His Ala Gly WO 01/00805 WO 0100805PCT/1IB0OI00926 180 185 190 Ser Ala Ala Pro Val Gly Val Cys Lys Ala Ala Gly Leu His Cys Ser 195 200 205 Arq Leu Asp Gly Ser Glu Leu Thr Tyr Asn Asn Lys Asp Thr Tyr Met 210 215 220 Pro Asp Ile Leu Ile Cys Arg Pro Glu Leu Ala Asp Glu Leu Leu Glu 225 230 235 240 Met Cys Ala Lys Phe Tyr Glu Glu Asn Gly Thr Tyr 245 250 <210> 7 <211> 875 <212> DNA <213> Corynebacterium glutamioum <220> <221> CDS <222> (101)..(859) <223> RXA01420 <400> 7 cgaagttcac gaotacaggc attacctggg tgctgatttt gcggaatgcg ctgctcatco acacqtgqaa tcctgattgg gggaqtaa agctgtggct ttq Le u acg Th r aac Asn aca Thr ttt Phe cac His tct Ser tac aat As n ttc Phe tgg Trp qtg Val gtt Val qc c Ala tac Tyr ctc tgg Trp att Ile ggO Gly tat Tyr t gg Trp 75 tqq T rp tac Tyr 9gC tog Ser ocqg Pro atc Ile gcg Ala 60 gac Asp ttt Phe tao Tyr gog gaa Glu aaa Lys gtq Val1 aag Lys toa Se r gaa Glu aoo Thr got qtg Val1 atg Met qtg Val1 ago Se r qqg Gly cog Pro att Ile aga Arg gaa otg Le u aag Lys otg Le u att Ile gto Val1 000 Pro oto Leu ggo aog otg ggt gga Thr Leu Gly Gly tto Phe ggo Gly 9gg Ala gag Glu qg Ala gtg Val ato Ile 115 atg 115 163 211 259 307 355 403 451 499 Tyr aac As n qct Al a 150 ctg Leu qgc Gly att Ile caq Gin ttc Phe 230 WO 01/00805 Leu Gly Va 120 acc aac at Thr Asn Ii 135 act tgg tt Thr Trp Ph cca atg gc Pro Met Al aag agt gq Lys Ser Gi 18 tcc ttt qc Ser Phe Al 200 cgc tat tt Arg Tyr Ph 215 tat gca qt Tyr Ala Va PCT/IBOO/00926 Met Phe Lys Phe Gly Ala Lys Leu Val Ala Glu 125 Giy 130 acc Th r gtg Val1 ttt Phe 170 gaa Gi u ttc Phe att Ile qtq Val atc Ile cca Pro 155.

gt t Val1 atc Ile tac Tyr qct Aila tgt Cys 235 gct gtt tct Ala Val Ser 145 tct gtc atc Ser Vai Ile qtg aga gac Val Arg Asp qtt ttq ttq Vai Leu Leu 195 qtc atg gtg Val Met Val 210 ttc ctt gc Phe Leu Gly 225 att ctc qcg Ile Leu Ala Vai Ser Vai 240 ctq tta cct tcq Leu Leu Pro Ser atg atc ctt Met Ile Leu taataaggcc acgaa <210> 8 (211> 253 <212> PRT <213> Corynebacterium giutamicun <400> 8 Vai Thr Leu Gly Giy Leu Asri Vai Pro 1 Met Leu Phe Tyr Leu Thr Phe Pro Leu 25 Val Lys Gly Val Gly Asn Trp Trp Ala 40 Ser Leu Ala Leu Ile Thr Val Ile His Gly Ile Glu Asn Phe Phe Val Pro Arg Pro Val Ala Giu Vai His Ala Asp Pro Ile Pro Vai Leu Giu Ser Tyr Trp Leu Le u Le u Thr Asp Thr Met Phe Gly Val1 Phe Gly As n Gin Pro WO 01/00805 WO 0100805PCT/1B00/00926 Arq Glu Ala 145 Se r Val1 Val Val1 Phe 225 Ile Le u Gly 130 Val1 Val1 Arg Leu Met 210 Leu Leu Ile 115 Met Se r Ile Asp Leu 195 Val1 Gly Ala 100 Giu Phe Phe Met Ile 180 Gly Phe T rp T rp Phe Lys Val1 Se r 165 Giu As n Val Ala Val 245 Tyr Asn Al a 150 Le u Gly le Gin Phe 230 Le u Le u Th r 135 Th r Pro Lys Ser Arq 215 Tyr Leu Gi y 120 Asn Trp Met Se r Phe 200 Tyr Al a Pro 105 Val1 Ile Phe Ala Gly 185 Al a Phe Val1 Ser Phe Th r Val1 Phe 170 Giu Phe Ile Val Le u 250 Gi y Ile Pro 155 Val Ile Tyr Al a Cys 235 Met Al a Pro 140 Le u Val1 Ala Met Gly 220 Phe Ile 110 Leu Ile Ph e Thr Pro 190 Gin Giu Val Val1 Ala Lys Leu 175 Arq Phe Tyr Ser Ala Le u Met 160 Ala Al a Pro Gi y ValI 240 '<210> 9 <211> 1983 <212> DNA <213> Corynebacterium qiutamicum <220> <221> CDS <222> (101)..(1960) <223> RXN02581 <400> 9 acggtcactc gctcaagtag tgttaqtttq caaaagtaat tggtcacaat aqtcattaaa gaattgtaag agqggttcac gcg Ala ttc Phe gct Ala gaa Glu ttc Phe gcc Aia gat Asp a ag Lys aaaatqttca tctttqtcga atq gat tta gat aaa Met Asp Leu Asp Lys 1 atc aac ctt cct cca Ile Asn Leu Pro Pro tac cag qct gac atc Tyr Gin Ala Asp Ile ttc tgg gac ttc tc Phe Trp Asp Phe Ser cqa aac gag atc gat Arq Asn Giu Ile Asp act Th r gg t Gly agc Ser ga t Asp gac Asp gtg Val1 150 etc Le u cca Pro g cg Ala cqa Arg gte Val 230 gtt Val1 ttt Phe ttc Phe agc Se r WO 01/00805 cgt ate aag Arg Ile Lys qat cgt gca Asp Arg Ala ttc: cte gqc Phe Leu Gly 105 cca aac gag Pro Asn Glu 120 agc gag cea Ser Giu Pro 135 ege aag eac Arg Lys His tct gta gat Ser Val Asp atq ctg ace Met Leu Thr 185 ccc att gat Pro Ile Asp 200 ace eca get Thr Pro Ala 215 ttg cag ate Leu Gin Ile tea tgg etg Ser Trp Leu gte act atg Val Thr Met 265 gtg cag eag Val Gin Gin 280 gac gtg gac Asp Val Asp 295 PCT/iBOOIOO926 get Ala geg Ala geg Ala eca Pro gtt ValI tte Phe tee Se r 170 gaa Gi u ctg Le u ggc Gi y tte Phe eca Pro 250 ctt Le u cc t Pro gt t Val1 gta Val1 75 ate Ile ate Ile gga Gly qt C Val1 tee Se r 155 t tg Leu gee Al a a cc: Thr gtt ValI age Ser 235 ctg Leu ggC Gi y tee Ser aac Asn gca Ala etg Leu tac Tyr ca e His gtt Val1 140 age Se r ect Pro gge Giy gca Ala gtt Val1 220 gee Ala cac His ctg Leu ege Arg gte Val1 300 ggc Gly get Ala get Ala gca Al a 125 ctg Le u ctt Leu ga t Asp ege Arg tte Phe 205 ctg Le u gea Ala ca e His gac Asp tgg Trp 285 tac Tyr egt Arg aae Asn ggC Gl y 110 gac Asp ace Th r eca Pro te t Se r ege Arg 190 et g Leu ace Th r cag Gin ga e Asp aae As n 270 att Ile ace Thr ttg Leu aac As n 95 atq Met cac His aae As n get Ala etc Le u 175 ctg Leu cag Gin aae As n ctg Le u atg Met 255 gag Giu aag Lys gtg Val1 cag eag gte gee ace etg Gin age Ser gte ValI ctc Le u tee Ser gea Al a 160 g eg Ala get Aila tac Tyr ege Arg aaa Lys 240 gge Giy ttc Phe cag Gin qtt Val1 Val Ala Thr Leu att Ile 100 ctt Leu tte Phe gg t Giy ege Arg gag Giu 180 eag Gln tee Se r ace Th r ege Arg geg Aia 260 eg c Arg ege Arg gee Ala ttC Phe tat Tyr gca Al a gee Aia ate: Ile 165 aac: As n tee Se r ace Th r aac As n etg Leu 245 9g Ala gat Asp gaa Glu etc Le u 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 WO 01/00805 PTIO/02 PCT/IBOO/00926 gag Giu 310 ctt Leu aac Asn gtt Val1 qtc ValI cg c A rg 390 aac As n cag Gin ca g Gin ctc Leu acc Thr 470 tac Tyr tac Tyr cac His atc Ile gca Ala qca Ala ctg Le u dcc Thr 360 acc Thr gcc Aia gct Ala gtc Val ggc Giy 440 cq c Arg ctg Le u atg Met acc Thr cca Pro 520 gca Ala gca cgc tac Ala Arg Tyr 315 ttq gat gcc Leu Asp Ala 330 dcc acc ttc Thr Thr Phe 345 ctg Cgt. cct Leu Arg Pro cca caq dcc Pro Gin Thr ttg gcc gaa Leu Ala Glu 395 gtt gct ttc Val Ala Phe 410 atc gtt gat Ile Val Asp 425 gaa atc tqq Giu Ile Trp gag gaa gac Giu Giu Asp gaa gaa aac Giu Giu Asn 475 qcc acc qgt Ala Thr Gly 490 ggt cgt ctg Giy Arq Leu 505 cag gac atc Gin Asp Ile gat tcc gtc Asp Ser Val gca aaq cca gca gag gga Aia Lys Pro Ala Giu Gly 320 atc Ile cg t Arg t cc Se r gaa Giu 380 aac As n gtc Val tcc Ser acc Th r acc Th r 460 tcc Se r ga c Asp aag Lys gag Giu g ca Ala 540 gg t Gly 335 ttc Phe ctt.

Le u cca Pro gag Giu Gly 415 gga Giy gaa Giu acc Thr gaa Gi u gtc Val1 495 atc Ile qtc ValI g ca Al a tcc Se r gag Giu gca Ala ctg Leu ctt Leu 400 cag Gin acc Th r dac As n ttc Ph e gg t Gly 480 atc Ile gtt Val1 cag Gin gt c Val1 gag Giu cct Pro gaa Giu atc Ile 385 gt a ValI gtt Val1 gag Giu act Th r cgc Ar q 465 qct Ala gta Val qt c Val1 gct Ala cca Pro 545 gag acc ctg gat Giu Thr Leu Asp 325 cca gtc aca gaa Pro Val Thr Giu 340 tac ggc ctg cct Tyr Gly Leu Pro 355 qca tcc ctg ctg Ala Ser Leu Leu 370 tcc tac ttc gac Ser Tyr Phe Asp gaa aag gqc aat Giu Lys Gly Asn 405 gca gcc cca cag Ala Ala Pro Gin 420 ctg gca gac ggc Leu Ala Asp Gly 435 gct gca ggt tac Ala Ala Gly Tyr 450 aac cgt ctg acc Asn Arg Leu Thr gcc gac gac aac Ala Asp Asp Asn 485 gac aac gag ctc Asp Asn Giu Leu 500 gca ggc cga aac Ala Gly Arq Asn 515 gct tcc gca cac Ala Ser Ala His 530 gqc gat gac att Gly Asp Asp Ile 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 WO 01/00805 gaa aaq ctc atc atc ctq qca Giu Lys Leu Ile Ile Leu Ala 550 555 gac gat gca gct qct gaa qaa Asp Asp Ala Ala Ala Glu Giu 570 cac ggt gtt gtt cca qaa gag His Gly Val Val Pro Glu Giu 585 gcg cgt tcc tcc tcc gqa aag Ala Arg Ser Ser Ser Gly Lys 600 tac att caq qaa caa gct aac Tyr Ile Gin Giu Gin Ala Asn 615 620 <210> <211> 620 <212> PRT <213> Corynebacteriun giutan gaa cgc gac acc act qca aac Giu Arg Asp Thr Thr Ala Asn 560 gca atc cgc tcc gcc gtt ggc Ala Ile Arg Ser Ala Val Gly 575 atc cgt atc ctc gca cct gac Ile Arg Ile Leu Ala Pro Asp 590 595 atc gca cgc cgc qtc aac cag Ile Ala Arg Arg Val Asn Gin 605 610 tagttctttg cagacaccgc agg PCT/iBOOIOO926 qcc 1795 Ala 565 gca 1843 Ala atc 1891 Ile aac 1939 Asn 1983 <400> Met Asp Leu Asp Lys Ala Ile Gly Ser 1 Ile Tyr Phe Arg Gin Pro Pro Asn Lys 145 Giu Asp Leu Ala Asp Giu Ala T yr Pro 115 Val1 Ala Pro Tyr Phe Ala Giu Thr Gly Ser Asp Asp Val1 150 Le u Pro Le u Giu Asp Arq Asp Phe Pro Se r 135 Arq Se r Met Leu Gly Asp Lys Ala Gi y 105 Glu Pro His Asp Th r G iu Gly Pro Ile Gly Ala Ala Ala 125 Leu Le u Asp Arq Asn Giu Arq Gin Arq Asn Gly 110 Asp Thr Pro Se r Arq Ala Th r Se r 225 Thr Ile Met Le u Pro 305 Gly Glu Pro Glu Ile 385 Val Val1 Glu Th r Arq 465 Ala Val WO 01/00805 180 Leu Arg Gin 195 Ser Gly Ser 210 Ile Leu Thr Pro Leu Arq Ile Leu Ala 260 Asn Pro Arq 275 Asn Arg Arg 290 Asn Phe Ala Glu Thr Leu Pro Vai Thr 340 Tyr Gly Leu 355 Ala Ser Leu 370 Ser Tyr Phe Giu Lys Gly Ala Ala Pro 420 Leu Ala Asp 435 Ala Ala Gly 450 Asn Arg Leu Ala Asp Asp Asp Asn Glu 500 Pro Thr 215 Leu Se r Val1 Val Asp 295 Leu Se r Ala Gin Thr 375 Giu Lys Le u Ile Asp 455 Arg Trp Ile PCTIBOOIOO926 Ile 200 Pro Gin Trp Thr Gin 280 Val Ala Ala Le u Th r 360 Th r Ala Ala Val1 Gly 440 Arg Le u Met Thr Ala Val1 220 Ala His Leu Arq Val 300 Ala Ile Arg Se r Giu 380 As n ValI Se r Thr Thr 460 Se r Asp Lys Phe 205 Le u Ala His Asp Trp 285 Tyr Lys Ile Gi u Tyr 365 As n Arq Ser Giu His 445 Ala Arg Leu Asp 190 Leu Th r Gin Asp As n 270 Ile Th r Pro Ile Ala 350 Gly Arq Vali Asn Th r 430 Gly Glu Ala Gly Le u 510 Gin Asn Leu Met 255 Glu Lys Val Ala GI y 335 Phe Le u Pro Giu Gly 415 Gly Gi u Th r Giu Val 495 Ile Tyr Arq Ly s 240 Gly Phe Gin Val Giu 320 Ser Gi u Ala Le u Le u 400 Gin Thr Asn Phe Gly 480 Ile Val1 WO 01/00805 PCTIIBOO/00926 Val Ala Gly Arg Asn His Tyr Pro Gin Asp Ile Giu Tyr Thr Val Gin 515 520 525 Ala Ala Ser Ala His Ile Arq Ala Asp Ser Val Ala Ala Phe Ala Val 530 535 540 Pro Gly Asp Asp Ile Glu Lys Leu Ile Ile Leu Ala Giu Arq Asp Thr 545 550 555 560 Thr Ala Asn Giu Ala Asp Asp Ala Ala Ala Glu Glu Ala Ile Arg Ser 565 570 575 Ala Val Gly Thr Ala His Gly Val Val Pro Glu Giu Ile Arg Ile Leu 580 585 590 Ala Pro Asp Giu Ile Ala Arg Ser Ser Ser Gly Lys Ile Ala Arq Arg 595 600 605 Val Asn Gin Arq Asn Tyr Ile Gln Giu Gin Ala Asn 610 615 620 <210> 11 <211> 1550 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(1527) <223> FRXA02581 <400> 11 gtc cct gtg ccg ctt tat qat cca aac gag cca gga cac gca gac cac 48 Val Pro Val Pro Leu Tyr Asp Pro Asn Giu Pro Gly His Ala Asp His 1 5 10 ctc aac gct gtt ttc gca gac aqc gag cca gtt gtc qtt ctq acc aac 96 Leu Asn Ala Val Phe Ala Asp Ser Giu Pro Val Val Val Leu Thr Asn 25 tcc aaq tcc qca ggt gcc gtq cgc aag cac ttc tcc agc ctt cca qct 144 Ser Lys Ser Ala Gly Ala Val Arq Lys His Phe Ser Ser Leu Pro Ala 40- gca gaa cgc cca cgc atc ctc tct gta gat tcc ttg cct gat tct ctc 192 Ala Giu Arg Pro Arg Ile Leu Ser Val Asp Ser Leu Pro Asp Ser Leu 55 gcg gat tct tac gag aac cca atg ctq acc qaa gcc ggc cgc cgc ctq 240 Ala Asp Ser Tyr Giu Asn Pro Met Leu Thr Giu Ala Gly Arg Arg Leu 70 75 gct gct ctg cgc cag tcc gcg ccc att gat ctq acc qca ttc ctg cag 288 Ala Ala Leu Arg Gin Ser Ala Pro Ile Asp Leu Thr Ala Phe Leu Gin 90 tac acc tcc ggc tcc acc cqa acc cca gct ggc gtt gtt ctg acc aac 336 Tyr Thr Ser Gly Ser Thr-Arq Thr Pro Ala Gly Val Val Leu Thr Asn 100 105 110 WO 01/00805 WO 0100805PCTIBOOIOO926 cqc Arg a aa Lys ggC Gi y 145 ttc Phe cag Gin gtt Val1 gag G iu tcC Se r 225 gag Giu gca Ala ctg Leu ctt Le u c ag Gin 305 acc Th r aac Asn atc Ile 115 cca Pro atc Ile aac As n aac Asn aac Asn 195 gag Giu cca Pro tac T yr qca Ala tcc Ser 275 gaa Giu gca Ala c tg Le u gct Ala ctg acc Leu Thr ctg cgc Leu Arg ctc gcg Leu Ala cca cgc Pro Arg 165 cgt cgc Arg Arg 180 ttc gcc Phe Ala acc ctg Thr Leu qtc aca Vai Thr ggc ctg Gly Leu 245 tcc ctg Ser Leu 260 tac ttc Tyr Phe aaq ggc Lys Gly gcc cca Ala Pro gca gac Ala Asp 325 gca ggt Ala Gly 340 aac gtc ttg cag atc ttc agc gcc gca cag ctg Asn Val Leu Gin Ile Phe Ser Ala Ala Gin Leu 120 gtt Vali 135 ttt Phe ttc Phe agc Se r gag Giu ctt Le u 215 aac As n gtt Val1 gtc Val1 cgc Arg aac Asn 295 cag Gin cag Gin ctc Le u tca Se r gtc Val1 gtg Val1 ga c Asp ctt Leu 200 t cc Se r gct Aia cag Gin acc Thr gag Giu 280 a ag Lys ctg Leu atc Ile gac Asp tgg Trp act Th r ca g Gin gtg Val1 185 gca Aia gca Al a ctg Le u acc Th r acc Th r 265 gcc Ala gct Aia gtc Val1 ggc Gly cgc Arg 345 ctg Le u atg Met cag Gin 170 gac Asp gca Aia ttq Le u acc Th r ct g Le u 250 cca Pro ttg Le u gtt Val1 atc Ile gaa Giu 330 gag Glu cca Pro ctt Le u 155 cct Pro gt t Vali cgc Arg ga t Asp acc Thr 235 cg t Arq cag Gin gcc Aia gct Ala gtt Val1 315 atc Ile gaa Glu 125 cac His ctg Le u cgc Arg qt C Val qca Ala 205 atc Ile Cgt Arq tcc Ser gaa Giu aac Asn 285 gt C Val tcC Se r acc Thr acc Th r cac His gaC Asp tgg Trp tac Tyr 190 aag Lys att Ile ga a Giu tac Tyr aac Asn 270 cgc Arg tcC Se r gaa Giu cac His gca Ala 350 gaC Asp aac Asn att Ile 175 acc Th r cca Pro atc Ile gct Ala ggt Gly 255 cgc Arg gt t Vali aac Asn acc Thr ggc Giy 335 gaa Glu atg Met gag Giu 160 aa g Lys gtg Vali gca Ala gg t Gly ttc Phe 240 ctt Le u cca Pro gag Giu ggc Gly gg a Gly 320 gaa Giu acc Thr 384 432 480 528 576 624 672 720 768 816 864 912 960 1008 1056 WO 01/00805 PCT/EBOO/00926 ttc cqc aac cgt ctg acc acc cgc ctq gaa qaa aac tcc cqc gca gaa 1104 Phe Arg Asn Arq Leu Thr Thr Arq Leu Glu Giu Asn Ser Arg Ala Glu 355 360 365 ggt gct qcc gac gac aac tac tgg atg gcc acc gqt gac ctc ggc gtc 1152 Gly Ala Ala Asp Asp Asn Tyr Trp Met Ala Thr Gly Asp Leu Gly Val 370 375 380 atc gta gac aac gag ctc tac atc acc ggt cgt ctg aaq gac ctc atc 1200 Ile Val Asp Asn Glu Leu Tyr Ile Thr Gly Arg Leu Lys Asp Leu Ile 385 390 395 400 gtt gtc gca ggc cqa aac cac tac cca cag gac atc qaq tac acc gtc 1248 Val Val Ala Gly Arg Asn His Tyr Pro Gin Asp Ile Glu Tyr Thr Val 405 410 415 cag gct qct tcc gca cac atc cgt gca gat tcc gtc gca gca ttc gca 1296 Gin Ala Ala Ser Ala His Ile Arg Ala Asp Ser Val Ala Ala Phe Ala 420 425 430 gtc cca ggc gat gac att gaa aag ctc atc atc ctg gca gaa cgc gac 1344 Val Pro Gly Asp Asp Ile Glu Lys Leu Ile Ile Leu Ala Giu Arg Asp 435 440 445 acc act gca aac gaa gcc gac gat gca gct qct qaa gaa gca atc cgc 1392 Thr Thr Ala Asn Glu Ala Asp Asp Ala Ala Ala Glu Giu Ala Ile Arq 450 455 460 tcc gcc gtt gqc act qca cac ggt gtt gtt cca gaa gag atc cgt atc 1440 Ser Ala Val Gly Thr Ala His Gly Val Val Pro Giu Glu Ile Arg Ile 465 470 475 480 ctc gca cct qac gag atc gcg cgt tcc tcc tcc gga aag atc gca cgc 1488 Leu Ala Pro Asp Giu Ile Ala Arg Ser Ser Ser Gly Lys Ile Ala Arg 485 490 495 cgc gtc aac cag cgc aac tac att cag gaa caa gct aac tagttctttg 1537 Arg Val Asn Gin Arg Asn Tyr Ile Gin Glu Gin Ala Asn 500 505 cagacaccgc agg 1550 <210> 12 <211> 509 <212> PRT <213> Corynebacterium glutanicun <400>' 12 Val Pro Val Pro Leu Tyr Asp Pro Asn Giu Pro Gly His Ala Asp His 1 5 10 Leu Asn Ala Val Phe Ala Asp Ser Giu Pro Val Vai Val Leu Thr Asn 25 Ser Lys Ser Ala Gly Ala Val Arg Lys His Phe Ser Ser Leu Pro Ala 40 Ala Giu Arg Pro Arg Ile Leu Ser Val Asp Ser Leu Pro Asp Ser Leu 55 Ala Al a T yr Arg Lys Gly 145 Phe Gln Val G lu Se r 225 Glu Ala Leu Le u Gin 305 Thr As n Phe Gly Ile WO 01/00805 Asp Ser Tyr Ala Leu Arg Thr Ser Gly 100 Ser Ile Leu 115 Thr Pro Leu 130 Ile Ile Leu Met Asn Pro Leu Asn Arg 180 Pro Asn Phe 195 Gly Giu Thr 210 Glu Pro Val Pro Tyr Gly Glu Ala Ser 260 Ile Ser Tyr 275 Val Giu Lys 290 Val Ala Ala Giu Leu Ala Thr Ala Ala 340 Arg Asn Arg 355 Ala Ala Asp 370 Val Asp Asn Glu Gin Ser Thr Arg Ala Arg 165 Arg Ala Leu Th r Le u 245 Le u Phe Gly Pro Asp 325 Gly Leu Asp Glu Asn 70 Ser Thr As n Le u Al a 150 Asp Glu Le u Asp Glu 230 Pro Le u Asp As n Gin 310 Gly Tyr Thr Asn Le u Pro Ala Arg Val Val1 135 Phe Phe Ser Glu Leu 215 Asn Val1 Val1 Arq As n 295 Gin Gin Le u Th r Tyr 375 Tyr Th r Asp Ala Ile Le u Met Gin 170 Asp Al a Le u Th r Le u 250 Pro Le u Val1 Ile Glu 330 Glu Glu Ala Gly Glu 75 Leu Gly Phe Pro Leu 155 Pro Val1 Arq Asp Thr 235 Arq Gin Ala Al a Val1 315 Ile Glu Giu Th r Arg PCTIBOO/00926 Le u Gln As n Le u Met Giu 160 Ly s Val Ala Gly Phe 240 Leu Pro Glu Gly Gi y 320 Glu Th r G lu Val1 Ile WO 01/00805 385 Val2 Gin Val1 Th r Se r 465 Le u Arq Gly Se r 420 Asp As n Gi y Asp Gin 500 His Ile Glu Asp 455 His Ala Tyr Tyr Arg Lys 440 Asp Giy Arg Ile Pro Al a 425 Le u Aila Val1 Ser Gin 505 Gin 410 Asp Ile Ala Vali Ser 490 Glu 395 Asp Se r Ile Ala Pro 475 Se r Gin Tyr Aia 430 Giu Al a Ile Ile Th r 415 Phe Arg Ile Arg Ala 495 PCT/IBOO/00926 400 Val1 Aia Asp Arg Ile 480 Arq <210> 13 <211> 4953 <212> DNA (213> Corynebacterium qiutamicum <220> <221> CDS <222> (4930) <223> RXA02582 <400> 13 tqqatqtcta ttctcctcag cctcgttttg gttqaqgcgg aqtttaaqtt gtaatcqagc tgaaaqqctq aqgcctcaat *tcg Ser ga t Asp ga c Asp t tg Le u atc Ile ga t Asp qtt Vali gca Ala gt Gi y tat Tyr cag Gin gtc Val1 atg Met gag Giu gag Giu acc Thr a cg Thr ttt Phe aac As n a cq Thr atagttttaa tattttttta atg gaa cag agc caa Met Giu Gin Ser Gin i cgc acc tgg ctc cgt Arq Thr Trp Leu Arq gag gaa gtt acq gat Giu Giu Val Thr Asp cgc qat gtt gtt gtg Arg Asp Val Val Val tcc ttg gat gcc acc Ser Leu Asp Ala Thr gcg cag cqc ctt gtt Ala Gin Arg Leu Val WO 01/00805 WO 0100805PCT/iBOO/00926 gag Gi u gcg Ala gct Ala ct C Le u tct Ser 150 tct Se r ttc Phe cg t Arq gca Ala tcc Se r 230 cat His att Ile gqa Ala cg t Arq gc a Ala 310 cca ggc Gly gtg ValI gcg Ala gtc ValI 135 gag Glu acc Thr ttt Phe att Ile cca Pro 215 aac As n cc t Pro aac As n tgt Cys aat As n 295 tct Se r acc gca Ala gg t Gly gct Ala ggt Gly 140 gag Glu ctg Le u ctc Le u acg Thr qgC Gly 220 a tg Met ggt Gly ttc Phe qta Val ca c His 300 act Th r gca cat His tcc Ser gag Glu 125 atc Ile gtt Val t ca Ser gag Glu tqg Trp 205 gaa Glu atg Met act Th r cgc Arg qcg Ala 285 gct Ala gca Ala ttc ttg Le u qtt Val1 atg Met 130 att Ile atg Met ttt Phe ga t Asp tac Tyr 210 ttc Phe cca Pro qtc Val aac As n gt c Val1 290 gtg Val1 gg t Gly ga t aa t Asn gtc Val 115 t gg Trp qqc Gly gaa Glu gat Asp cc t Pro 195 gct Al a ata Ile qca Ala gct Al a gtg Val 275 cg t Arg aac Asn gtg Val1 gg t 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 Pro cgt Arg a tc Ile aac Asn caq Gin 390 a cc Thr gac Asp qac Asp cac His gct Ala 470 aac Asn cca Pro gcg Ala aac Asn gac Asp 550 cg a Arg WO 01/00805 Thr Giy Lys tcc gac ggc Ser Asp Giy 345 cqc gac ggc Arg Asp Gly 360 tcc gat ggt Ser Asp Giy 375 gtt gat gtg Val Asp Val acc gtg gat Thr Val Asp ccc att gaa Pro Ile Glu 425 gca tcc acc Ala Ser Thr 440 act gag tca Thr Giu Ser 455 ttg cag aac Leu Gin Asn cgc tac atc Arg Tyr Ile cgt gaa tgg Arq Giu Trp 505 ttc ggg ttc: Phe Gly Phe 520 gca gaa gac Ala Giu Asp 535 cag cag gtt Gin Gin Val caa gca gct Gin Ala Ala Ile 330 gcc Al a gac Asp cac His ct g Le u tac Tyr 410 gca Ala cca Pro gc t Ala aag Lys qa t Asp 490 ccg Pro qgt Gly tac Tyr gcc Ala gc t Ala His gga Gi y aag Lys tcC Se r ca g Gin 395 qt t Val1 act Th r act Th r g cg Ala act Th r 475 ttc Phe gaa Giu qqa Gly gaa Glu ctg Le u 555 qac Asp Ala gtc Val atc Ile aac Asn 380 cg t Arq gag Glu gcc Ala ctt Leu gg t Gly 460 ctg Leu gat Asp t ac T yr acc Thr a cc Th r 540 ccg Pro ttg Leu Phe gtt Val1 atc Ile 365 gga Gly gca Al a gct Ala ct g Leu ctg Leu 445 at t Ile cca Pro gct Ala aac .Asn aac Asn 525 cgt Arg gtg Val gcg Ala Se r gtg Val1 350 gt Gly ctg Le u tat Tyr cac His ggt Gly 430 gg C Gly gca Ala cca Pro gag Glu gg t Gly 510 g cg Ala gcc Ala t ct Ser gac Asp Asp 335 c tg Le u gtg Val1 acc Thr gt t Val1 ggc Gly 41i5 gct Ala tct Ser gg t Gly acc Thr c gt Arg 495 cac His cac His ccg Pro gg t Giy ttc Phe Asp a ag Lys atc Ile gct Ala qac Asp 400 acc Th r gtt Val gca Al a gt c Vai gt g Val1 480 ttg Le u gca Ala gt g Val aag Lys cac His 560 ttg Le u Ala cg c Arg aag Lys cca Pro 385 gct Aia Gly ttg Le u aag Lys atc Ile 465 aac Asn ga a G lu gta ValI gtg Val1 gag Giu 545 ct g Le u gag Glu Asp gtg Val1 ggt Gi y 370 aac As n cag Gin acc Th r gg c Gly tdc Se r 450 aag Lys ttt Phe gt t Val1 gc t Ala atc Ile 530 g cg Ala cca Pro ggc Gly Gly ga t Asp 355 tct Ser cct Pro gtt Val1 atc Ile tat T yr 435 aac Asn gtg Val1 gc a Al a gtg Val1 gg t Gly 515 tct Ser t tg Leu tcc Se r cgc Arg Phe 340 ga c Asp gca Ala ga c Asp ga t Asp ctg Le u 420 ggc Gi y ttc Phe ctg Leu gg t Gly gaa Glu 500 g tg Val1 gag Giu ctt Leu a gg Arg aaa Lys PCTIBOO/00926 Val gca 1171 Ala gtg 1219 Val1 gca 1267 Ala ccc 1315 Pro 405 ggt 1363 Gly cgt 141i Arg ggc i459 Gi y ctt 1507 Le u cca 1555 Pro 485 gat 1603 Asp tct 1651 Se r tac 1699 Tyr cct 1747 Pro cgt 1795 Arg 565 gat 1843 Asp WO 01/00805 WO 0100805PCT/1B00/00926 tg c Cys cg c Arg cg c Arg gct Al a 630 gq a Gi y ca g Gin tcc Se r gac Asp act Thr 710 atg Met gac Asp gag Glu gtg Val1 gag Glu 790 gac Asp tcc Se r c tg Le u 615 gat Asp tcc Se r ttc Phe ggc Gly acc Thr 695 gac Asp tcg Se r cgc Arg ggc Gly ga a Glu 775 tac Tyr cta Le u cgc Arg 600 cgc Arg t cc Se r cag Gin cgc Arg t gg T rp 680 gag Giu ctg Le u atg Met gat Asp gag G iu 760 ttc Ph e aag Lys acc Thr 585 gca Al a ca g Gin cca Pro ca c His gag Giu 665 tcc Se r acc Th r ttg Le u gg t Gly a cc Thr 745 aag Lys gcc Al a ggc Gly 570 575 cca gta gcc cgc gcg ctg Pro Val Ala Arg Ala Leu 590 gtc gtg ctt gca agc act Val Val Leu Ala Ser Thr 605 gtc gca gaa ggc aag gtc Val Ala Glu Gly Lys Val 620 gcg gcc aac ggt cct gtg Ala Ala Asn Gly Pro Val 635 cgc ctc atg atc aag gaa Arg Leu Met Ile Lys Giu 650 655 cgc atc gaa gag ctc gat Arg Ile Giu Glu Leu Asp 670 atc atg aaa ctt gtt ttg Ile Met Lys Leu Val Leu 685 gcg cag gtt gtg atc acc Ala Gin Val Val Ile Thr 700 gca agc ttc ggt gta aag Ala Ser Phe Gly Val Lys 715 gag atc gct gcc gct tac Giu Ile Ala Ala Ala Tyr 730 735 atg ctc att gcc agc cac Met Leu Ilie Ala Ser His 750 tcc ctg gct gag gat cag Ser Leu Ala Giu Asp Gin 765 gct gcg gac ctg gat aag Ala Ala Asp Leu Asp Lys 780 att gag cct gct gtt tat Ile Glu Pro Ala Val Tyr 795 cct cgc gac gcc gtg gtt Pro Arg Asp Ala Val Val 810 815 gca Ala atc Ile agc Se r ttt Phe 640 ttg Le u gaa Giu gat Asp gca Ala cca Pro 720 gct Ala cg t Arg ctg Le u ttc Phe gca Ala 800 ca g Gin gg c Gly gag Giu gtc Val1 625 gtg Val1 tgc Cys atg Met gat Asp atc Ile 705 gct Ala gcc Ala tcc Se r ggt Gly atc Ile 785 ggc Gly ttc Ph e cgc Arg gaa Glu 610 gg t Gly tac Tyr tca Se r gt t Val gag Giu 690 cag Gin gcc Ala ggt Gi y cgc Arg gcc Ala 770 ga a Glu cca Pro gta Val aat Asn 595 gcc Ala atc Ile t cc Se r att Ile aag Lys 675 caa Gin atc Ile gtc Val gg a Gly cta Le u 755 atg Met gaa Giu Gly gag Glu 580 cat His gta Val tcc Ser ggt Gly t cg Se r 660 ttc Phe act Th r gca Ala a tg Met ct c Leu 740 atg Met gct Al a aac Asn atg Met aag Lys 820 ggc Gi y aag Lys gca Ala ttc Phe 645 cca Pro gag Gl u tac Tyr ctg Le u ggc Gly 725 agc Se r gg c Gly gtg Val1 cc t Pro act Th r 805 ctg Le u 1891 1939 1987 2035 2083 2131 2179 2227 2275 2323 2371 2419 2467 2515 2563 acc gtg ggt gga Thr Val Gly Gly WO 01/00805 WO 0100805PCTIBOOIOO926 gaa tct gaa gac aag ttt Glu Ser Giu Asp Lys Phe 825 cac acc tct qct gtg gaa His Thr Ser Ala Val Glu 840 gct ggc atc gag cca ctt Ala Gly Ile Glu Pro Leu 855 gat cag qqt gtt acc tac Asp Gin Gly Val Thr Tyr 870 875 tac atg ctc cgc tgt acc Tyr Met Leu Arq Cys Thr 890 gaa gct gca ttc qct gca Glu Ala Ala Phe Ala Ala 905 aac cca qtt qca ctc atg Asn Pro Val Ala Leu met 920 aaq cct gat gca cag ctg Lys Pro Asp Ala Gin Leu 935 gca gaa tcc ctc cgc gac Ala Giu Ser Leu Arg Asp 950 955 aac gtt qat ttc tcc gcg Asn Val Asp Phe Ser Ala 970 cca cac atc acc tgq aag Pro His Ile Thr Trp Lys 985 tcc tct ggc gca tcc ctg Ser Ser Gly Ala Ser Leu 1000 aac aac act gtq gcg ttt Asn Asn Thr Val Ala Phe 1015 gtg gca atc atg gaa gca Val Ala Ile Met Giu Ala 1030 10 g cc Ala cqc ctg ctc aac gtc aaq Arg Leu Leu Asn Val Lys 830 ggc qct ggc Gly Ala Gly 835 2611 cca ctt ctt Pro Leu Leu 845 ccg ctg cag Pro Leu Gin 860 cca gtt gga Pro Val Gly cgc cag tcc Arg Gin Ser gqc cac aac Gly His Asn 910 ggc atg atg Gly Met Met 925 ctg ttc agc Leu Phe Ser 940 ctt ctg gcg Leu Leu Ala ctg tac ggc Leu Tyr Gly cac caq cgc His Gin Arg 990 gat ctg ccg Asp Leu Pro 1005 tcc acc gca Ser Thr Ala 1020 gcc gcc atg Ala Ala Met 35 ggt gag ctc gct ggc Gly Giu Leu Ala Gly 850 atc ccg ttg ttc aqc Ile Pro Leu Phe Ser 865 gca gtg gtc cac gac Ala Val Val His Asp 880 gtg tac ttc cag qac Val Tyr Phe Gin Asp 895 acc ttq gtg gaa att Thr Leu Val Glu Ile 915 aac acc gcg ttc acc Asn Thr Ala Phe Thr 930 ctg aag cga aaa gtc Leu Lys Arq Lys Val 945 aag ctg tac gtc aac Lys Leu Tyr Val Asn 960 qaa ggt gaa acc atc Glu Gly Glu Thr Ile 975 ttc tgg act tcc gca Phe Trp Thr Ser Ala 995 gqc ttc cgc gtq aat Gly Phe Arg Val Asn 1010 gcc gaa ctq gca cca Ala*Glu Leu Ala Pro 1025 gct gtc acc cca ggt Ala Val Thr Pro Gly 1040 ctg cca cca agc ggc Leu Pro Pro Ser Gly 1055 gaa atc 2659 Giu Ile tct gtc 2707 Ser Val qcc gat 2755 Ala Asp 885 tcc acc 2803 Ser Thr 900 tcc ccg 2851 Ser Pro gtg ggc 2899 Val Gly cca gag 2947 Pro Glu ggc gca 2995 Gly Ala 965 gat ccg 3043 Asp Pro 980 cgc cca 3091 Arg Pro ctg cca 3139 Leu Pro tcc gca 3187 Ser Ala tcc tcc 3235 Ser Ser 1045 gaa atc 3283 Glu Ile 1060 gtc gac gca gtg gac gag cgc gac atg Val Asp Ala Val Asp Giu Arg Asp Met 1050 WO 01/00805 PCTABOO0/00926 acc acc atc gtg act cgc tcc ttg gqc ggt ttg aqc ctg tcc gta tac 3331 Thr Thr Ile Val Thr Arg Ser Leu Gly Gly Leu Ser Leu Ser Val Tyr 1065 1070 1075 aag att gag gga acc acc agc act ctt gtc gcc gaa qgt ttc gca gcc 3379 Lys Ile Glu Gly Thr Thr Ser Thr Leu Val Ala Giu Gly Phe Ala Ala 1080 1085 1090 aac cca gga ttc gcc gca gca tct tcc ttc gac ggt cca ggc tac gac 3427 Asn Pro Gly Phe Ala Ala Ala Ser Ser Phe Asp Gly Pro Gly Tyr Asp 1095 1100 1105 gga ttc aac acc gat tac agc gac cag cca gac cct cqc tcc gac ctg 3475 Gly Phe Asn Thr Asp Tyr Ser Asp Gin Pro Asp Pro Arg Ser Asp Leu 1110 1115 1120 1125 cct ttg gac atc gaa gca gtc cgc tgg gac cca gcg acc gaa acc gtc 3523 Pro Leu Asp Ile Glu Ala Val Arg Trp Asp Pro Ala Thr Giu Thr Val 1130 1135 1140 gaa gag cgc atg cqc gcc atc gtc tcc gaa gca atg ggc tac gac gtg 3571 Glu Glu Arg Met Arg Ala Ile Val Ser Glu Ala Met Gly Tyr Asp Val 1145 1150 1155 gat gac ctc cca cqc gaa ctc cca ctg att gac ctc ggc ctc gac tcc 3619 Asp Asp Leu Pro Arg Giu Leu Pro Leu Ile Asp Leu Gly Leu Asp Ser 1160 1165 1170 ctc atg ggc atg cgc atc aaa aac cgc atc gaa aat gac ttc cag atc 3667 Leu Met Gly Met Arg Ile Lys Asn Arg Ile Giu Asn Asp Phe Gin Ile 1175 1180 1185 cca cca ctc cag gtc caa gcg ctc cgc gat gca tcc gtc gct gac gtg 3715 Pro Pro Leu Gin Val Gin Ala Leu Arg Asp Ala Ser Val Ala Asp Val 1190 1195 1200 1205 gta atc atg gtg gaa aac atg gtc gct ggc cgc tcc tct gag acg ctt 3763 Val Ile Met Val Giu Asn Met Val Ala Gly Arg Ser Ser Giu Thr Leu 1210 1215 1220 gtc gac gcc acc ccg cag gtg cca gct gag gca gca ggg gag gct caa 3811 Val Asp Ala Thr Pro Gin Val Pro Ala Giu Ala Ala Gly Giu Ala Gin 1225 1230 1235 gct gct gag tct tct gct tcg gqc gag gac gtg cag ggc gtt ggc gtt 3859 Ala Ala Giu Ser Ser Ala Ser Gly Glu Asp Val Gin Gly Val Gly Val 1240 1245 1250 gca ccg cga gat gcg tcg gaa cgc atg gtc ttt ggt act tgg gca ggc 3907 Ala Pro Arg Asp Ala Ser Glu Arg Met Val Phe Gly Thr Trp Ala Gly 1255 1260 1265 ctg act ggc gct gcg gca gct ggc gtg acc agc aag ttg cca cag atc 3955 Leu Thr Gly Ala Ala Ala Ala Gly Val Thr Ser Lys Leu Pro Gin Ile 1270 1275 1280 1285 gat gtc gat act gca acc gca att gcc gag cgc ctc acc gag cgt tcc 4003 Asp Val Asp Thr Ala Thr Ala Ile Ala Giu Arg Leu Thr Glu Arg Ser 1290 1295 1300 gga att gaa atc agc act gaa cag gtg ttg gct gca gaa acc ctc gaa 4051 WO 01/00805 PCT/IBOO/00926 Gly Ile Glu Ile Ser Thr Glu Gin Val Leu Ala Ala Giu Thr Leu Glu 1305 1310 1315 cca ctg tct gac etg qtg cgt qaa gqe ctc gaa act qaa gtt cag gqc 4099 Pro Leu Ser Asp Leu Val Arg Giu Gly Leu Giu Thr Giu Val Gin Gly 1320 1325 1330 aac att cqt qtg ctg cgt gga cgt gca qaa gqc tcc acc aag cct qca 4147 Asn Ile Arg Val Leu Arg Gly Arg Ala Giu Gly Ser Thr Lys Pro Ala 1335 1340 1345 gtq ttc atg ttc cac cca get gqc ggt tct tee gtg gtc tac caa cca 4195 Val Phe Met Phe His Pro Ala Gly Gly Ser Ser Val Val Tyr Gin Pro 1350 1355 1360 1365 cta atg cgt egt ctq cct gaa gat gtc cct gte tat ggc gtt gag cgt 4243 Leu Met Arg Arg Leu Pro Giu Asp Val Pro Val Tyr Giy Vai Giu Arg 1370 1375 1380 ctg gaa ggc gat etc qet gac cqc geg gca gca tat qtt gat gac atc 4291 Leu Glu Gly Asp Leu Ala Asp Arg Ala Ala Ala Tyr Val Asp Asp Ile 1385 1390 1395 aag aag tac tec gat gqc ttc eca gta gtt ttg ggt ggc tgg agc ttc 4339 Lys Lys Tyr Ser Asp Gly Phe Pro Val Val Leu Gly Gly Trp Ser Phe 1400 1405 1410 gge ggt gcc gtt gcc ttc gag qtt gcc cac caa ctg gtt ggc tcc gat 4387 Gly Gly Ala Val Ala Phe Giu Val Ala His Gin Leu Val Gly Ser Asp 1415 1420 1425 qtt gag gta get acc gtq gcg ttg ctq gat act gtq cag cet tca aac 4435 Val Glu Val Ala Thr Val Ala Leu Leu Asp Thr Val Gin Pro Ser Asn 1430 1435 1440 1445 cca gca cca gat acc get gag gaa act cgt gca cqc tgg act cgc tac 4483 Pro Ala Pro Asp Thr Ala Giu Glu Thr Arg Ala Arg Trp Thr Arq Tyr 1450 1455 1460 gcg gac ttc gcc aaq aag acc tac ggc ctt gat ttc gag gta cct ttt 4531 Ala Asp Phe Ala Lys Lys Thr Tyr Gly Leu Asp Phe Giu Val Pro Phe 1465 1470 1475 gaa atc ttg gac acc atc ggt gaa gac gqa atg ctg tec atg atg acc 4579 Glu Ile Leu Asp Thr Ile Gly Giu Asp Gly Met Leu Ser Met Met Thr 1480 1485 1490 gac ttc etg gcc aac acc gac gcc tcc gag eac gga ttg tee gea ggt 4627 Asp Phe Leu Ala Asn Thr Asp Ala Ser Giu His Gly Leu Ser Ala Gly i495 1500 1505 gtg ett gaa cac cag cgc get tet ttt gtg gac aac cgc ate etg get 4675 Val Leu Glu His Gin Arq Ala Ser Phe Val Asp Asn Arg Ile Leu Ala 1510 1515 1520 1525 aaa ett aat ttt gea gac tgg gee aac gtt gaa gee cct gte ate ctg 4723 Lys Leu Asn Phe Ala Asp Trp Ala Asn Val Glu Ala Pro Val Ile Leu 1530 1535 1540 ttc cgc geg qaa cgc atq cat gat gga get ate qaa ett gaa cca aac 4771 Phe Arq Ala Glu Arg Met His Asp Gly Ala Ile Giu Leu Glu Pro Asn WO 01/00805 WO 0100805PCT/IBOO/00926 1545 tat qcc aag att Tyr Ala Lys Ile 1560 ttg gaa att gtt c Leu Giu Ile Val C 1575 cca gaa atc gqc a Pro Giu Ile Giy T 1590 att tct cgg aaq a Ile Ser Arq Lys P~ 1 la t ksp :ag lin Lca h r iat ~sn .610 1550 1555 caa qat gga qga tgg tcc gga att gtc aaC Gin Asp Gly Gly Trp Ser Gly Ile Val Asri 1565 1570 ctq aat gqt qac cac ctgqgca gtt qtc gat Leu Asn Gly Asp His Leu Ala Vai Vai Asp 1580 1585 gtc gga gct cat ttg aqt cgc cgc att gat Val Giy Ala His Leu Ser Arg Arg Ile Asp 1595 1600 taqtaacqqa qaqctqacqq aag 4819 4867 I 4915 1605 4953 <210> 14 <211> 1610 <212> PRT <213> Corynebacterium giutamicum <400> 14 Met Giu Gin Ser Gin Ser Ser Asp Gin 1 Arg Glu Arg Se r Ala G lu Ala Asp Pro 145 Lys Th r Gi u Asp Le u Gin Le u Val Met 130 Ile Met T rp Val1 Val Asp Arg Asn Val 115 T rp Giy Glu Leu Th r Vali Ala Leu Phe 100 Gly Lys Arg Gi u 5 Arg Asp Vali Thr Val1 Ser Met Leu T rp Phe 165 Asp Asp Leu Ile 70 Giu Ala Ala Leu Se r 150 Ser Trp Ly s Se r 55 Ala Gly Val1 Ala Val1 135 Giu Th r Val1 Ala 40 Gly T yr Giu Ser Arg 120 Giu Tyr Ile Val1 25 Met Giu Glu Pro Asp 105 Tyr Gly Ala Gly Lys Arg Giu Leu Tyr Arg Ser Pro Arg Gi y Gi y 170 Met Thr Thr Giu Pro Arq Pro Gi y Asp Asp 155 Ty r Th r Thr Phe Asn Thr Ala Gly Ala Gly 140 Giu Leu Val1 Gly Gly Le u Ile His Ser Glu 125 Ile Val1 Se r Giu Ile Le u Leu Arg Th r His 110 Se r Se r Met Asp Ser Phe Asp Ala Glu Phe Phe Gly Leu Ser Pro Leu Glu Ala Ala Asn WO 01/00805 Asp Pro Gin 195 Tyr Ala Arg 210 Phe Ile Gly Pro Ala Glu Val Ala Asn 260 Asn Val Asp 275 Val Arg Ala 290 Val Asn Ile Gly Val Ile Asp Gly Phe 340 Val Asp Asp 355 Gly Ser Ala 370 Asn Pro Asp Gin Val Asp Thr Ile Leu 420 Gly Tyr Gly 435 Ser Asn Phe 450 Lys Val Leu Phe Ala Gly Val Val Glu 500 Ala Gly Val Gin Ile Ser Ala 245 Arg Thr Leu Leu Ser 325 Val Ala Val Ala Pro 405 Gly Arg Gly Leu Pro 485 Asp Ser Arg Ala Ser 230 His Ile Ala Arg Ala 310 Pro Arg Ile Asn Gin 390 Thr Asp Asp His Ala 470 Asn Pro Ala Ile Pro 215 Asn Pro Asn Cys Asn 295 Ser Thr Ser Arg Ser 375 Val Thr Pro Ala Thr 455 Leu Arg Arg Phe Leu 200 Asn Asn Tyr Tyr Ser 280 Gly Pro Gly Asp Asp 360 Asp Asp Val Ile Ser 440 Glu Gin Tyr Glu Gly Thr Gly 220 Met Gly Phe Val His 300 Thr Ala Val Ile Asn 380 Arg Glu Ala Leu Gly 460 Leu Asp Tyr Thr Trp 205 Glu Met Thr Arg Ala 285 Ala Ala Phe Val Ile 365 Gly Ala Ala Leu Leu 445 Ile Pro Ala Asn Asn Glu Ala Ile Ser Gly 270 Val Ile Phe Ser Val 350 Gly Leu Tyr His Gly 430 Gly Ala Pro Glu Gly 510 Ala Ala Val Ala Ser 255 Pro His Ala Ala Asp 335 Leu Val Thr Val Gly 415 Ala Ser Gly Thr Arg 495 His His PCT/IB00/00926 Leu Gly Ala 240 Ala Ser Gin Gly Glu 320 Asp Lys Ile Ala Asp 400 Thr Val Ala Val Val 480 Leu Ala Val WO 01/00805 WO 0100805PCT[JiB00I00926 Val Glu 545 Leu Glu Gly Glu Val1 625 ValI Cy s Met Asp Ile 705 Ala Al a Se r Gi y Ile 785 Gly Phe ValI Se r Le u Se r Arg Asn 595 Ala Ile Ser Ile Lys 675 Gin Ile Val Gly Leu 755 Met Glu Gly Giu Giy 835 Tyr Pro Arg 565 Asp Gly Lys Ala Phe 645 Pro Glu Tyr Leu Gi y 725 Ser Gly Vai Pro Thr 805 Leo Giy As n Asp 550 Arg Cys Arq Arg Ala 630 Gly C in Se r Asp Thr 710 Met Asp Glu Val1 Glu 790 Thr Glu His Al a 535 Gln Gin Asp Ser Leo 615 Asp Ser Phe Gly Th r 695 Asp Se r Arg Gly Giu 775 Tyr Val Ser Th r 520 Glu Gin Ala Leo, Arq 600 Arg Se r Gin Arq Trp 680 G lu Leo Met Asp Glu 760 Phe Lys Gly Glu Ser 840 Asp Val1 Ala Thr 585 Ala Gin Pro His Glu 665 Ser Thr Leu Gly Thr 745 Lys Ala Gly Gly Asp 825 Ala Tyr Ala Ala 570 Pro Val1 ValI Ala Arg 650 Arq Ile Ala Ala Giu 730, Met Se r Ala Ile Pro 810 Lys Val Glu Leo 555 Asp Val Val1 Al a Ala 635 Leu Ile Met Gin Se r 715 Ile Leu Leu Ala Glu 795 Arg Phe Glu Thr 540 Pro Leo Ala Leo Glu 620 As n Met Giu Lys Val1 700 Phe Ala Ile Ala Asp 780 Pro Asp Ala Pro 525 Arq Val Ala Arq Ala 605 Giy Gly Ile Glu Leo 685 Val1 Gb y Al a Ala Glu 765 Leu Ala Ala Arg Leu 845 Ala Se r Asp Ala 590 Ser Lys Pro Lys Leu 670 Val Ile Val Ala Ser 750 Asp Asp Val1 Val1 Leo 830 Leo Pro Gly Phe 575 Leu Th r Val1 ValI Giu 655 Asp Leu Th r Lys Tyr 735 His Gin Lys T yr Val1 815 Leo Gly Lys His 560 Leo Ala Ile Se r Phe 640 Leo Giu Asp Ala Pro 720 Ala Arq Leo Phe Ala 800 Gin Asn Glu WO 01/00805 PCT/IB00/00926 Leu Ala Gly Glu Ile Ala Gly Ile Glu Pro Leu Pro Leu Gin Ile Pro 850 855 860 Leu Phe Ser Ser Val Asp Gin Gly Val Thr Tyr Pro Val Gly Ala Val 865 870 875 880 Val His Asp Ala Asp Tyr Met Leu Arg Cys Thr Arg Gin Ser Val Tyr 885 890 895 Phe Gin Asp Ser Thr Glu Ala Ala Phe Ala Ala Gly His Asn Thr Leu 900 905 910 Val Glu Ile Ser Pro Asn Pro Val Ala Leu Met Gly Met Met Asn Thr 915 920 925 Ala Phe Thr Val Gly Lys Pro Asp Ala Gin Leu Leu Phe Ser Leu Lys 930 935 940 Arg Lys Val Pro Glu Ala Glu Ser Leu Arg Asp Leu Leu Ala Lys Leu 945 950 955 960 Tyr Val Asn Gly Ala Asn Val Asp Phe Ser Ala Leu Tyr Gly Glu Gly 965 970 975 Glu Thr Ile Asp Pro Pro His Ile Thr Trp Lys His Gin Arg Phe Trp 980 985 990 Thr Ser Ala Arg Pro Ser Ser Gly Ala Ser Leu Asp Leu Pro Gly Phe 995 1000 1005 Arg Val Asn Leu Pro Asn Asn Thr Val Ala Phe Ser Thr Ala Ala Glu 1010 1015 1020 Leu Ala Pro Ser Ala Val Ala Ile Met Glu Ala Ala Ala Met Ala Val 1025 1030 1035 1040 Thr Pro Gly Ser Ser Val Asp Ala Val Asp Glu Arg Asp Met Leu Pro 1045 1050 1055 Pro Ser Gly Glu Ile Thr Thr Ile Val Thr Arg Ser Leu Gly Gly Leu 1060 1065 1070 Ser Leu Ser Val Tyr Lys Ile Glu Gly Thr Thr Ser Thr Leu Val Ala 1075 1080 1085 Glu Gly Phe Ala Ala Asn Pro Gly Phe Ala Ala Ala Ser Ser Phe Asp 1090 1095 1100 Gly Pro Gly Tyr Asp Gly Phe Asn Thr Asp Tyr Ser Asp Gin Pro Asp 1105 1110 1115 1120 Pro Arg Ser Asp Leu Pro Leu Asp Ile Glu Ala Val Arg Trp Asp Pro 1125 1130 1135 Ala Thr Glu Thr Val Glu Glu Arg Met Arg Ala Ile Val Ser Glu Ala 1140 1145 1150 Met Gly Tyr Asp Val Asp Asp Leu Pro Arg Glu Leu Pro Leu Ile Asp 1155 1160 1165 WO 01/00805 PCT/IB00/00926 Leu Gly Leu Asp Ser Leu Met Gly Met Arg Ile Lys Asn Arg Ile Glu 1170 1175 1180 Asn Asp Phe Gin Ile Pro Pro Leu Gin Val Gin Ala Leu Arg Asp Ala 1185 1190 1195 1200 Ser Val Ala Asp Val Val Ile Met Val Glu Asn Met Val Ala Gly Arg 1205 1210 1215 Ser Ser Glu Thr Leu Val Asp Ala Thr Pro Gin Val Pro Ala Glu Ala 1220 1225 1230 Ala Gly Glu Ala Gin Ala Ala Glu Ser Ser Ala Ser Gly Glu Asp Val 1235 1240 1245 Gin Gly Val Gly Val Ala Pro Arg Asp Ala Ser Glu Arg Met Val Phe 1250 1255 1260 Gly Thr Trp Ala Gly Leu Thr Gly Ala Ala Ala Ala Gly Val Thr Ser 1265 1270 1275 1280 Lys Leu Pro Gin Ile Asp Val Asp Thr Ala Thr Ala Ile Ala Glu Arg 1285 1290 1295 Leu Thr Glu Arg Ser Gly Ile Glu Ile Ser Thr Glu Gin Val Leu Ala 1300 1305 1310 Ala Glu Thr Leu Glu Pro Leu Ser Asp Leu Val Arg Glu Gly Leu Glu 1315 1320 1325 Thr Glu Val Gin Gly Asn Ile Arg Val Leu Arg Gly Arg Ala Glu Gly 1330 1335 1340 Ser Thr Lys Pro Ala Val Phe Met Phe His Pro Ala Gly Gly Ser Ser 1345 1350 1355 1360 Val Val Tyr Gin Pro Leu Met Arg Arg Leu Pro Glu Asp Val Pro Val 1365 1370 1375 Tyr Gly Val Glu Arg Leu Glu Gly Asp Leu Ala Asp Arg Ala Ala Ala 1380 1385 1390 Tyr Val Asp Asp Ile Lys Lys Tyr Ser Asp Gly Phe Pro Val Val Leu 1395 1400 1405 Gly Gly Trp Ser Phe Gly Gly Ala Val Ala Phe Glu Val Ala His Gin 1410 1415 1420 Leu Val Gly Ser Asp Val Glu Val Ala Thr Val Ala Leu Leu Asp Thr 1425 1430 1435 1440 Val Gin Pro Ser Asn Pro Ala Pro Asp Thr Ala Glu Glu Thr Arg Ala 1445 1450 1455 Arg Trp Thr Arg Tyr Ala Asp Phe Ala Lys Lys Thr Tyr Gly Leu Asp 1460 1465 1470 Phe Glu Val Pro Phe Glu Ile Leu Asp Thr Ile Gly Glu Asp Gly Met 1475 1480 1485 Leu Ser Met Met Thr Asp Phe Leu Ala Asn Thr Asp Ala Ser Glu His WO 01/00805 PCTIBOO/00926 1490 1495 1500 Gly Leu Ser Ala Gly Val Leu Glu His Gin Arg Ala Ser Phe Val Asp 1505 1510 1515 1520 Asn Ar Ile Leu Ala Lys Leu Asn Phe Ala Asp Trp Ala Asn Val Giu 1525 1530 1535 Ala Pro Val-Ile Leu Phe Arg Ala Giu Arg Met His Asp Gly Ala Ile 1540 1545 1550 Giu Leu Giu Pro Asn Tyr Ala Lys Ile Asp Gin Asp Gly Gly Trp Ser 1555 1560 1565 Gly Ile Val Asn Asp Leu Glu Ile Val Gin Leu Asn Gly Asp His Leu 1570 1575 1580 Ala Val Val Asp Giu Pro Giu Ile Gly Thr Val Gly Ala His Leu Ser 1585 1590 1595 1600 Arg Arg Ile Asp Giu Ile Ser Arg Lys Asn 1605 1610 <210> <211> 540 <212> DNA <213> Corynebacterium giutamicun <2 <221> CDS <222> (101)..(517) <223> RXA01138 <400> cttattcaag tttttgqaaa qataattaac tatqact gctgcgagac actqtcqqat cgttcccttc aqqtgtc ga t Asp t cq Ser gtt Val t cg Se r aac As n cac His atg Met gag Glu ga t Asp qt t Val1 75 qgq Gly gtq Val1 cag Gin t tg Le u tcc Ser ~tta aaaacaqtaa acggaacgca :acg gtq qtc acc act acc Val Val Thr Thr Thr 1 agt qcg ttc qtq tcc ttq Ser Ala Phe Val Ser Leu atc gat aag aaa tca agc Ile Asp Lys Lys Ser Ser qtt qct gtg tct gtg tta Val Ala Val Ser Val Leu ttt ggc cgt cat ctg gaa Phe Gly Arg His Leu Glu tca aca aac agq gca qcq Ser.Thr Asn Arg Ala Ala 80 WO 01/00805 gtc ttg qaa ggt qcg tca gca tgq t Val Leu Giu Gly Ala Ser Ala Trp L.

tac cca gqt gga gat cac ttt att a Tyr Pro Gly Gly Asp His Phe Ile I 105 1 qct cac qac qag gag caa aag cca c Ala His Asp Giu Glu Gin Lys Pro L.

120 125 ttt cag tqq caa qaa gat taattctcc Phe Gin Trp Gin Giu Asp 135 <210> 16 <211> 139 <212> PRT <213> Corynebacterium glutainicum <400> 16 Vai Val Thr Thr Thr Asp Gly Glu\ 1 5 Ala Phe Vai Ser Leu Ser Leu GiuE Asp Lys Lys Ser Ser Val Val Pro 1 40 Ala Vai Ser Val Leu Ser Giu GiuC Gly Arg His Leu Giu Asn Lys Phe 70 Thr Asn Arg Ala Ala Vai Leu Giu Ala Val Vai Asp Lys Tyr Pro Gly 100 Ala Val Giu Giu Cys Ala His Asp 115 120 His Arg Gly Arq Leu Phe Gin Trp 130 135 <210> 17 <211> 756 <212> DNA <213> Corynebacterium giutarnicum <220> <221> CDS (101)..(733) <223> RXA01980 P4 tg agt ggc gct gtg gtq gat aaa ~eu Ser Gly Ala Val Vai Asp Lys 95 100 .tc acc att gcc gtg gaa gag tqt ie Thr Ile Ala Val Giu Giu Cys .10 1.15 :tt ctt tac cac cgt ggc agg ctt ~eu Leu Tyr His Arg Giy Arq Leu 130 ~a ccccttcatt ttc -T/1B00100926 403 451 499 540 ~al 'ro 25 'he ;n %s p 105 Glu Gln Le u Le u Gi y Ala Ile Trp Ile Pro 125 Th r Val1 Se r Ile Lys Leu Ile 110 Le u Val1 Ser Pro Th r Arg Se r Thr Le u Ser Ile Val Phe Ser Giy Ile Tyr WO 01/00805 <400> 17 aggatcgtac caaataaggc aataaactct ttttactttt PCTIBOOI0O926 cctcaacttc ctgaaaagtc qccgccctag aattcactaa gtttccqata tctttaaccc atg cag atc att gat Met Gin Ile Ile Asp ctc Leu ga t Asp atg Met cc t Pro gag Giu cta Le u atc Ile aaa Lys cat His cct Pro 150 att Ile att Ile cgt t ct Se r caa Gin cat His gca Ala gaa Giu t gt Cys qaa Giu caa Gin qcc Aia 135 tca Se r gaa Giu gct Ala cca cat His gaa Giu caa Gin cat His acg Thr act Th r cca Pro gqq Gi y 120 cg t Arg ctg Leu aac As n tqt Cys at t ttc gcg Phe Aia aag acg Lys Thr aga ctt Arg Leu qat cca Asp Pro tta ccc Leu Pro gct. gat Ala Asp tcc ttc Ser Phe gca ccc Ala Pro gtc att Vai Ile gca cag Ala Gin 155 acc aat Thr Asn 170 tgg cca Trp Pro ctc gtc Leu Val ccc qga Pro Giy gtc tcc Val Ser gtt ggt Vai Giy 45 caa ggt Gin Gly ctt tat Leu Tyr ata gaa Ile Giu gtc gca Vai Aia ggc tgq Gly Trp 125 gcc att Ala Ile 140 cgc tgg Arg Trp ttg gac Leu Asp gtt cca Val Pro cca gag Pro Giu 205 cct Pro ga t Asp cat His cag Gin tct Se r aca Thr t gg Trp tta Leu 130 ccc Pro ga c Asp acg Thr agt Se r act Th r 210 ggg Giy Gly gta Val1 atc Ile cg c Arg cac His gaa Giu 115 ga a Giu ga t Asp cat His gg t Gly ttc Ph e 195 cgc Arg Arg Pro Ile 200 taaagcgaac ggcagcacct tct WO 01/00805 PCT/IB00/00926 <210> 18 <211> 211 <212> PRT <213> Corynebacterium glutamicum <400> 18 Met Gin Ile Ile Asp Leu Ser His Ala Phe Ala Pro Gly Gin Pro His 1 5 10 Tyr Pro Gly Asp Pro Asp Gin Glu Ile Lys Thr Val Ser Thr Ile Glu 25 Asn Asp Gly Phe Leu Met His Gin Tyr Arg Leu Val Gly Pro Trp Gly 40 Thr His Val Asp Ala Pro Ala His Phe Asp Pro Gin Gly Arg Thr Leu 55 Asp Gin Ile Pro Val Glu Glu Thr His Leu Pro Leu Tyr Cys Leu Arg 70 75 Phe Ser Arg Pro Asp Leu Cys Thr Ala Ala Asp Ile Glu Ala Phe Glu 90 His Thr His Gly Lys Ile Glu Pro Gly Ser Phe Val Ala Leu His Thr 100 105 110 Gly Trp Glu Trp Gly Lys Gin Gly Ile Ala Pro Gly Trp Ser Ile Glu 115 120 125 Ala Leu Glu Ile Leu His Ala Arg Gly Val Ile Ala Ile Gly His Asp 130 135 140 Leu Pro Asp Thr Asp Pro Ser Leu Glu Ala Gin Arg Trp Trp Leu Cys 145 150 155 160 Arg Asp His Trp Gin Ile Glu Asn Leu Thr Asn Leu Asp Lys Val Pro 165 170 175 Ala Thr Gly Ala Met Ile Ala Cys Pro Trp Pro Val Pro Lys Asp Gly 180 185 190 Ala Ser Phe Pro Val Arg Pro Ile Ala Leu Val Pro Glu His Leu Ser 195 200 205 Pro Thr Arg 210 <210> 19 <211> 1830 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1807) <223> RXN01007 <400> 19 WO 01/00805 tctgaaccaa iaiaccgatc aqictaaaaq tgtgitaaqi PCTIIBOO/00926 tctggaacai aaaitagciq acacgtaaag taaciiaaaq aiicaciqga gqiaagccta gtg itt aaa aag cac Val Phe Lys Lys His aga Arg cqq Arg tcc Se r iac Tyr tt Phe tac Tyr cic Le u acc Th r gig Val acc Th r 150 gag Glu cag Gin gag Glu gg i Gly tc Phe tqc Cys gag Gi u ccc Pro gat Asp ga a Glu gic Val1 120 a ac Asn tct Ser acc Th r acc Th r gcc Ala 200 cic Le u acc Thr aca Thr cca Pro aac Asn cct Pro gi a Val1 105 acc Tb r tc Pbe gag Giu gtc Val agc Ser 185 aai Asn ggc Gi y g cg Al a g ca Ala cag Gin Gly gaa Giu aac Asn iac Tyr gati Asp cag Gin cga Arg 170 icc Se r gag Glu tcc Se r gcc Ala caa Gin tic Phe agi Se r 75 acc Thr gaa Glu icc Se r cic Le u aic Ile 155 iic Pbe tic Phe gati Asp acc Tb r a cg Thr 30 caa Gin acc Th r aac As n ci c Le u a cg Tb r 110 acc Tb r cic Le u i ac Ty r ici Ser ggc Gly 190 cca Pro aaa cca Lys Pro 15 ctg gca Leu Ala gca gaa Ala Glu ctg iac Leu Tyr aac ati Asn Ile 80 aag ccg Lys Pro 95 gaa iii Giu Phe ccg ctg Pro Leu qgc gat Gly Asp gac cac Asp His 160 gag cct Giu Pro 175 cii tat Leu Tyr ggc aac Gly Asn gaa acc Glu Tbr 1 cg c Arg gga Gly gac Asp cca Pro gca Al a igg Trp acc Tb r a cg Tbr caa Gin 145 ggc Gly qca Ala gc c Ala gcc Ala i ca Se r ttg Le u aac As n cca Pro gac Asp aic Ile tic Pb e gci Aia 130 gat Asp ga a Glu cct Pro gat Asp caa Gin 210 ata Ile qca Al a a cg Tb r i ca Se r cgc Arg qcc Al a aac Asn 115 ga a Glu cga Arg gi a Val ggi Gly icc Se r 195 aac Asn a cc Tb r gic Val cic Le u g cg Ala tg Le u acc Tbr 100.

aic Ile aac Asn cgc Ar g gi a Val1 iii Pbe 180 acc Tb r gic Val cgc Arg ci g Le u aci Tb r Gly cic Le u gaa Giu cg c Arg gig Val ci c Le u gat Asp 165 gct Ala tig Le u aic Ile 115 163 211 259 307 355 403 451 499 547 595 643 691 739 787 ggc icc ggi Gly Ser Gly 215 cci tic gig aic acc gat Pro Phe Val Ile Tbr Asp 220 cia ggc acc aac cic Leu Gly Thr Asn Leu 225 WO 01/00805 WO 0100805PCT/IBOO/00926 acc Thr 230 cat His qa a Glu gcc Al a atc le ttc Ph e 310 ctg Le u gat Asp tac Tyr qct Al a cg c Arg 390 cag Gin ga t Asp ga c Asp ttg Le u caa Gin gaa Giu cgc Arg c cg Pro 295 cgc Arg atc Ilie t cc Ser aaa Lys ctq Leu 375 a ag Lys cct Pro ctg Le u gct Ala act Thr gg t Gly tcc Ser ca g Gin 280 atc Ile ttc Phe cac His tat Tyr gaa Giu 360 ctt Le u gac Asp cg t Arg ggc Gi y ga c Asp 440 qcg Ala cg c Arg gtc Val 265 atc Ile atc Ile aaa Lys gcg Ala ccg Pro 345 caa Gin gac Asp gg t Gly t ca Ser atc Ile 425 tcc Ser cg t Arg g cg Ala 250 cgc Arg gaa Giu t cc Se r aac Asn at c Ile 330 ctg Leu qta Val1 qaa Giu gaa G lu cgc Arg 410 a ag Lys a ag Lys gag Giu 235 a ag Lys atc Ile gcg Al a gca Ala gag Glu 315 ga c Asp gca Al a qa t Asp gcc Ala ctt Le u 395 gaa Gi u gtc Val1 ga t Asp ga t Asp ctt Leu gga Gly cca Pro gcc Al a 300 ctg Le u cgc Arg act Thr gcc Al a ggc Gly 380 cta Le u gt t Val1 aac Asn ctc Le u tac Ty r gac Asp gcc Al a gtg Val1 285 acc Th r ctc Le u ga a Glu tct Ser tat Ty r 365 t g Trp gag Glu gt c Val1 ctc Leu aac Asn 445 ga t Asp gcc Ala atc Ile 270 gag Glu aac As n tca Ser aag Ly s gtg Val1 350 gt c Val1 acc Thr ctc Leu acc Thr aac Asn 430 a ag Lys t gg T rp gtc Val1 255 gtt Val gca Ala ggt Gly ga c Asp atc Ile 335 ctt Le u tac Tyr ctt Leu acc Th r atg Met 415 cca Pro atc Ile gca Al a 240 aat As n gct Ala cac His gtg Val1 atc Ile 320 atg Met gcg Ala ga c Asp ga t Asp ttc Ph e 400 gtc Val1 gg t Gly ca q Gin cca Pro tat Tyr ggc Gi y ctg Leu aac Asn 305 cgt Arg cgt Arg caa Gin cta Le u agc Ser 385 aac Asn caa Gin ga c Asp gt t ValI cca tca Pro Ser gtc ctc Val Leu caa ggt Gin Gly 275 aag gat Lys Asp 290 aac agc Asn Ser gtt cgc Val Arg gtg ctg Val Leu aat gca Asn Ala 355 gac aaa Asp Lys 370 gac ggc Asp Gly gaa gcc Glu Ala gaa cag Giu Gin caa gca Gin Ala 435 cgc cac Arg His 450 cgc Arg g cg Al a 260 ga t Asp gca Al a ttc Phe caa Gin ttt Phe 340 ctg Leu g ca Al a atg Met ctc Leu ctc Leu 420 gcc Ala acc Th r ga a Gi u 245 gg t Gly atc Ile Gly aac As n gc t Al a 325 agt Se r qgc Gi y aca Thr cg t Arg cca Pro 405 gg t Gly ca g Gin atg Met 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 gtg ggt cgc gca gac tat Val Gly Arg Ala Asp Tyr 455 gac gtg ctg aaa tcc cag ctg tac tcc acc Asp Val Leu Lys Ser Gin Leu Tyr Ser Thr 460 465 WO 01/00805 cqc aac gag Arg Asn Glu ggc gat cct Gly Asp Pro cgc gaa gag Arg Glu Glu 505 acc gag cag Thr Glu Gin 520 ggc gtg cag Gly Val Gin 535 ccc agc ttc Pro Ser Phe gag gag gac Glu Giu Asp ctg ttg aac atg acc gtg gaa Leu Leu Asn Met Thr Val Giu 475 480 cat ttg gag gaa ctc ctc atg His Leu Glu Glu Leu Leu Met 490 495 gac cgt gca gca gca tct gcc Asp Arg Ala Ala Ala Ser Ala 510 gca tat gtt ctt cca ctg ttt Ala Tyr Val Leu Pro Leu Phe 525 cct tac gtg aag ggc ttt agc Pro Tyr Val Lys Gly Phe Ser 540 tat gag acc tac att gac cat Tyr Giu Thr Tyr Ile Asp His 555 560 taaatgacta cctcgcagat tct ggg Gly gct Ala g ca Al a ga a Giu ccc Pro 545 tcc Se r gag Glu att Ile gca Ala gag Gi u 530 gaa Giu agc Ser PCTIBOOIOO926 gat 1555 Asp 485 agc 1603 Se r tac 1651 Tyr gtc 1699 Val ggc 1747 Gi y tcc 1795 Se r 565 1830 <210> <211> 569 <212> PRT <213> Corynebacterium glutamicui <400> Val Phe Lys Lys His Arg His Gly Leu 1 Arg Ser Ile Thr Arg Arg Phe Phe Thr Gly Leu Ala Val Leu Ser Gly Cys Thr 40 Asp Asn Thr Leu Thr Tyr Leu Glu Pro 55 Pro Pro Ser Ala Giy Phe Tyr Pro Asn 70 Ala Asp Arg Leu Leu Tyr Gin Asp Pro Trp Ile Ala Thr Giu Leu Pro Glu Val 100 105 Thr Phe Asn Ilie Arg Thr Asp Val Thr 115 120 Thr Ala Glu Asn Val Val Lys Asn Phe 130 135 Pro Ala Pro Phe Val1 Le u Asp Asp Tyr 140 Giu Ala Ser Arg Val Giu Ala Gly 125 Gly Pro Ala Giu Tyr Ile Pro Phe Le u Asp WO 01/00805 WO 0100805PCTIIBOO/00926 Gin 145 Gl y Al a Al a Al a Le u 225 Pro Tyr Gi y Le u As n 305 Arg Arg Gin Leu Ser 385 Asn Gin Asp Val Asp Giu Pro Asp Gin 210 Gly Pro Val Gin Lys 290 Asn Val1 Val1 As n Asp 370 Asp Giu Giu Gin Arg 450 Arg Val1 Gi y Se r 195 As n Thr Se r Leu Gly 275 Asp Ser Arg Leu Al a 355 Lys Gly Ala Gin Ala 435 His Arq Val1 Phe 180 Thr ValI As n Arg Ala 260 Asp Ala Phe Gin Phe 340 Le u Al a Met Le u Le u 420 Ala Th r Le u Asp 165 Ala Leu Ile Le u Gl u 245 Gi y Ile Gi y As n Al a 325 Se r Gi y Th r Arg Pro 405 Gly Gin Met Thr 150 Glu Gin Glu Gi y Thr 230 His Giu Al a Ile Phe 310 Le u Asp Tyr Aila Arq 390 Gin Asp Asp Val Ile Asp Ala Phe Se r 215 Le u Gin Glu Arq Pro 295 Arg Ile Se r Lys Le u 375 Lys Pro Le u Ala Gi y 455 Se r Thr Thr Al a 200 Giy Thr Gly Ser Gin 280 Ile Phe His Tyr Giu 360 Leu Asp Arg Gly Asp 440 Arg Glu Vali Se r 185 As n Pro Al a Arq Val 265 Ile Ile Lys Ala Pro 345 Gin Asp Gl y Se r Ile 425 Se r Ala Gin Arg 170 Se r Glu Phe Arq Al a 250 Arg Giu Se r As n Ile 330 Le u Val1 Giu Giu Arg 410 Lys Lys Asp Ile 155 Phe Phe Asp Val Glu 235 Lys Ile Aila Ala Giu 315 Asp Al a Asp Ala Leu 395 Glu Val1 Asp T yr Th r His As n Phe Ile 220 Asp Le u Gl y Pro Al a 300 Leu Arg Thr Ala Gly 380 Le u Val1 Asn Le u Asp 4 As n Phe Ala Ala 205 Th r T yr Asp Ala Val1 285 Thr Le u Giu Se r Tyr 365 Trp Giu Val Le u Asn 445 Val1 Asp Giu 175 Le u Gly Gi u Trp Val1 255 Val1 Ala Gly Asp Ile 335 Le u Tyr Le u Thr Met 415 Pro Ile Lys His 160 Pro Tyr As n Thr Ala 240 As n Ala His ValI Ile 320 Met Aia Asp Asp Phe 400 Val1 Gi y Gin Ser WO 01/00805 Leu Tyr Ser Giu Thr Ala Ile Ala Ser 500 Ala Gin Asp 515 Giu Pro Val 530 Giu Val Ile Ser Asp His Th r Asp 485 Se r Tyr Val1 Gi y Ser 565 Leui 475 Leu Arg Tyr Tyr Glu 555 PCT/iBOOIOO926 Glu 480 Met Al a Phe Se r His 560 <210> 21 <211> 858 <212> DNA <213> Corynebacterium qiutamicum <220> <221> CDS <222> (101) (8 <223> RXN00784 (400> 21 gtcacatcaq ttatcgcgcg gaatagttag cggqcgcatq atctattcca actacgcgta aaacgaggac gtttaaagct tcc Se r ccc Pro ttt Phe a tg Met gcc Ala caa Gin aaa Lys atc Ile gga Gi y qaa Giu Gly atg Met t qq Trp ga c Asp tac Tyr tcc Se r gcc Al a 80 Ct t Le u tggttgqat atatqaataa atg tct att gaa ttc Met Ser Ile Glu Phe 1 agc qac atc atg tgc Ser Asp Ile Met Cys qac qcc cta agt acc Asp Ala Leu Ser Thr aaq agc ttc gaa ctc Lys Ser Phe Glu Leu gac gtt qaa tac ctc Asp Val Glu Tyr Leu cgc caa atq aac ggc Arg Gin Met Asn Gly qaa atq aat cct gac Glu Met Asn Pro Asp WO 01/00805 WO 0100805PCTIBOO/00926 ga a Glu gog Ala qc t Ala too Se r 150 cto Le u gc Ala aaa L ys qta Val1 ttt ace Thr aaa Ly s cac His 135 att Ile qaa Gi u cgo Arg tao Tyr gaa Gi u 215 gag atc Ile gc Ala 120 ttc Phe gct Al a too Se r caa Gin gcc Ala 200 aa a Lys gtc gcg Ala 105 ca o His qt a Val1 qca Al a gao Asp ctc Le u 185 ato Ile gec Ala att gco Al a ggc Gi y gac Asp gag Glu gtg Val1 170 qg C Gly aac As n ttc Phe gac aac As n aag Lys ggc Gly gt t Val 155 tac Tyr gtc ValI Gly gaa Glu ggc ace Thr ca a Gin aag Lys 140 gg t Gly ace Th r caa Gin gec Al a gag Glu 220 caa ate Ile ca a Gin 125 aac As n oto Le u aac Asn ggt Gly caa Gin 205 t gg Trp ago Se r aao Asn 110 ga a Glu gtt ValI gat Asp gaa Glu gtg Val 190 caa Gin gca Ala t gc C ys cac His gog Ala gao Asp agt Ser 160 caa Gin ttc Phe ga a Glu gaa Glu gtc Vali ogo Arg oag Gin ctc Le u 145 gca Ala caa Gin ttt Phe gta Val aao Asn 225 gao Asp ott Leu gaa Glu 130 gat Asp gcc Ala gao Asp gta Val ttc Phe 210 ooa Pro ggo Gly Phe Glu Val 230 Ile Asp Giy Gin 235 taaottttgg gaootatgtg ogt <210> 22 <211> 245 <212> PRT <213> Corynebacterium giutamioum <400> 22 Met Ser Ile Giu Phe Ser Ala Pro Ala 1 5 Ser Asp Ile Met Cys Pro Phe Cys Tyr 25 Asp Ala Leu Ser Thr Phe Asp Gln Ala 40 Lys Ser Phe Glu Leu Met Pro Giy Leu 55 Asp Val Giu Tyr Leu Ala Asp Ala Lys 70 Met Lys le Giu Val Gly Lys Lys Arg Leu Arg Ile Giu Val Giu Thr His Pro Leu Arg Met Ser Leu Giu Gin Arg Giu Arg Gin Le u 145 Ala Gin Phe Val Asn 225 Asp WO 01/00805 Gin Met Asn Met Asn Pro 100 Leu Thr His 115 Glu Leu Phe 130 Asp Val Leu Ala Arg Glu Asp Val His 180 Vai Phe Asp 195 Phe Thr Gly 210 Pro Val Ser Giy Thr Cys PCTIBOOIOO926 Gly Asp Phe Lys Val1 Ala 165 Glu Arg Th r Pro Asn 245 Gin Glu Ala Ala Se r 150 Le u Ala Lys Val Phe 230 Val1 Th r Lys His 135 Ile Glu Arg Tyr Glu 215 Glu Gin Ala Ile Ala 105 Ala His 120 Phe Val Ala Ala Ser Asp Gin Leu 185 Ala Ile 200 Lys Ala Val Ile Met 90 Ala Gly Asp Glu Val1 170 Gly As n Phe Asp Ala As n Lys Gi y Val1 155 Tyr Val1 Gly Glu Gly 235 Gin Thr Gin Lys 140 Gly Th r Gin Al a Glu 220 Gin Aia Ile Gin 125 Asn Le u Asn Gly Gin 205 Trp Se r Thr Asn 110 Giu Val1 Asp Glu Val 190 Gin Ala C ys Gly Ala Val1 Asp Ala Val1 175 Pro Glu Ala Se r Leu His Ala Asp Se r 160 Gin Phe Giu Giu Val1 240 <210> 23 <211> 1896 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1873) <223> RXA02335 <400> 23 tqgttqagtt cttcggggtt gagtgtgcaa gaatattcac gtctacccac tgagtcatca atttaaatca qgagttatta aqq aag atc acc a-ag gtt ctt gtc Arg Lys Ile Thr Lys Val Leu Val cqc gtg ttc cgt gca gct cga gat Arq Val Phe Arq Ala Ala Arg Asp tac gca gag cca gat gca gat gca qct aac cqt Ala Asn Arg 15 qaa ggc atg Glu Gly Met 30 cca ttc gtg tattggtcag gcaactatgt gtg tca gtc gag act Val Ser Val Glu Thr 1 ggt gag att gca atc Gly Giu Ile Ala Ile gga tct gtc gcc gtc Gly Ser Val Ala Val tca tat gca gac gag 115 163 211 259 WO 01/00805 PCT1IiB0000926 Tyr Ala Glu Pro Asp Ala Asp Ala Pro Phe Val Ser Tyr Ala Asp Glu 45 gct ttt goc otc ggt ggc caa aca toc got gag too tao ctt gto att 307 Ala Phe Ala Leu Gly Gly Gln Thr Ser Ala Glu Ser Tyr Leu Val Ile 60 gac aag ato atc gat gog goo cgc aag tcc ggc gcc gao gc atc cac 355 Asp Lys Ile Ile Asp Ala Ala Arg Lys Ser Gly Ala Asp Ala Ile His 75 80 ccc ggc tao ggc tto ctc gca gaa aac got gac ttc gca gaa gca gto 403 Pro Gly Tyr Gly Phe Leu Ala Glu Asn Ala Asp Phe Ala Glu Ala Val 95 100 ato aac gaa ggc ctg atc tqg att gga oct tca cot gag too atc cgo 451 Ile Asn Glu Gly Leu Ile Trp Ile Gly Pro Ser Pro Glu Ser Ile Arg 105 110 115 too otc ggo gao aag gto aoo got cgc cao ato goa gat aoo goo aag 499 Ser Leu Gly Asp Lys Val Thr Ala Arg His Ile Ala Asp Thr Ala Lys 120 125 130 got cca atg got cot ggc aoo aag gaa cca gta aaa gao goa goa gaa 547 Ala Pro Met Ala Pro Gly Thr Lys Glu Pro Val Lys Asp Ala Ala Glu 135 140 145 qtt gtg got tto got gaa gaa tto ggt otc cca ato goo ato aag gca 595 Val Val Ala Phe Ala Glu Glu Phe Gly Leu Pro Ile Ala Ile Lys Ala 150 155 160 165 got tto ggt ggc ggo gga ogt ggc atg aag gtt gco tao aag atg gaa 643 Ala Phe Gly Gly Gly Gly Arg Gly Met Lys Val Ala Tyr Lys Met Glu 170 175 180 gaa gto got gac ctc ttc gag too gca aco cgt gaa gca aco gca gog 691 Glu Val Ala Asp Leu Phe Glu Ser Ala Thr Arg Glu Ala Thr Ala Ala 185 190 195 ttc ggc cgo ggo gag tgo tto gtg gag cgo tao otg gao aag gca cgo 739 Phe Gly Arg Gly Glu Cys Phe Val Glu Arg Tyr Leu Asp Lys Ala Arg 200 205 210 cac gtt gag got oag gtc ato gc gat aag cac ggc aac gtt gtt gtc 787 His Val Glu Ala Gln Val Ile Ala Asp Lys His Gly Asn Val Val Val 215 220 225 gc gga aco cgt gao tgo too otg cag cgo cgt tto cag aag otc gtc 835 Ala Gly Thr Arg Asp Cys Ser Leu Gin Arg Arg Phe Gln Lys Leu Val 230 235 240 245 gaa gaa gca oca goa oca ttc otc aco gat gao cag cgo gag ogt otc 883 Glu Glu Ala Pro Ala Pro Phe Leu Thr Asp Asp Gln Arg Glu Arg Leu 250 255 260 cac too too gog aag got ato tgt aag gaa got ggc tao tao ggt gca 931 His Ser Ser Ala Lys Ala Ile Cys Lys Glu Ala Gly Tyr Tyr Gly Ala 265 270 275 ggo aco gtt gag tao otc gtt ggc too gao ggc ctg ato too tto otc 979 Gly Thr Val Glu Tyr Leu Val Gly Ser Asp Gly Leu Ile Ser Phe Leu WO 01/00805 280 qtc aac ac Val Asn V1 295 ggc atc ga Gly Ile As ctc tcc at Leu Ser Ii cgc atc aa Arg Ile As 34 aag atc ac Lys Ile Th 360 tcc qgt gt: Ser Gly Va 375 ctq gca aa Leu Ala L cgc tcc cq Arg Ser Ar gtt atc cc Vai Ilie Pi 42 aac gac qa Asn Asp Gi 440 qat aac cc Asp Asn Pr 455 gag gac aa Giu Asp L cgc gtt ga Arg Vai G1 qgt cct aa Gly Pro L PCT/IBOO/00926 cg c Arg ctg Le u aag Lys 330 gg c Gly ag c Se r gtt ValI ct g Leu cg t Arg 410 ttc Phe qgc Gly atc Ile acc Th r gt t Val 490 aag Ly s ctc Leu gtc Val1 315 gaa Glu gaa G iu tac T yr qaa Giu atc Ile 395 g ca Ala cac His i tc Phe qca Ala c ca Pro 475 gca Ala aag Lys cag Gin 300 cqc Arg ga t Asp gac Asp cg c Arg qgt Gi y 380 gtt ValI ct t Leu cag Gin gag Giu cc t Pro 460 qca Al a ctc Le u gcc Ala ca c His ttc Phe cca Pro 335 t cc Ser ca g Gin atc Ile ga c Asp ta C Tyr 415 gtg Val1 acc Th r gac Asp gtt Val1 ga t Asp 495 cg t Arq cca Pro cq C Arg 320 cgc Arg aac Asn gg C Giy tcC Se r acc Th r 400 gtt Val1 ga a Giu aag Lys gct Al a gtt Val 480 ct g Le u cg C Arg gtC Val1 305 atc Ile Gly ttc Phe cca Pro gga Gly 385 cgc Arg gtc Val aac Asn tgg Trp t cc Se r 465 gtg Val1 qca Ala gca Ala 290 acc Thr g ca Ala cac His a tg Met ggc Gly 370 ca g Gin gag Giu gag Giu cca Pro atc Ile 450 gag Giu gag Giu ctc Leu ggt Gly 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 gct ggc gta Ala Gly Val 520 tcc ggc gat gca gtg gca gct Ser Gl.y Asp Ala Val Ala Ala 525 cca atg cag ggc act gtC Pro Met Gin Gly Thr Vai 530 WO 01/00805 atc aag gtc aac gtc gaa gaa gqc Ile Lys Val Asn Val Glu Glu Gly 535 540 gtt gtt qtc ctc gag gct atg aag Val Val Val Leu Glu Ala Met Lys1 550 555 aaq tcc gga acc gta acc ggc ctt Lys Ser Gly Thr Val Thr Gly Leu 570 aac aag ggc gtt gtt ctc ctc gag Asn Lys Gly Val Val Leu Leu Glu 585 agt (210> 24 <211> 591 <212> PRT <213> Corynebacterium glutamicum PCTIBOOIOO926 gaa gtc aac gaa ggc gac acc 1747 Glu Val Asn Glu Gly Asp Thr 545 gaa aac cct gtg aag gct cat 1795 Glu Asn Pro Val Lys Ala His 560 565 gtc gct gca ggc gag ggt gtc 1843 Val Ala Ala Gly Giu Gly Val 575 580 aag taagttcttg gattcctgtt 1893 Lys 1896 <400> 24 Val Ser Val Glu Thr Arg 1 GI y Gly Se r Ser Ala Phe Pro Ala Lys 145 Ile Ala Giu Ser Tyr Tyr Asp Ala Glu Asp 130 Asp Ala Tyr Al a Ala Asp Val1 I le Al a 100 Ile Ala Ala Lys Met 180 Ile ValI Glu Ile His Val1 Arg Lys Glu Ala 165 Glu Arg T yr Ala Asp 70 Pro Ile Se r Ala Val1 150 Al a Glu Lys Val1 Ala Phe 55 Lys Gly As n Le u Pro 135 Val1 Phe Val Val1 Ala Ala Gly Ala Le u Ile Val1 Gi y Glu 155 Gi y Phe Le u Arg Asp Gin Al a Al a Trp Th r Thr 140 Glu Arg Glu Val Asp Ala Thr Arg Glu Ile Ala 125 Lys Phe Gly Se r WO 01/00805 WO 0100805PCT/BOOIOO926 G lu Leu Gly 225 Phe Gin Giy Le u Val1 305 Ile Gly Phe Pro Giy 385 Arg Val As n Trp Se r 465 Val1 Ala Ala Asp 210 As n Gin Arg Ty r Ile 290 Th r Al a His Met Giy 370 Gin Giu Giu Pro Ile 450 Giu Glu Le u Thr 195 Lys Val1 Lys Glu Tyr 275 Se r Glu Giu Ala Pro 355 Vali Phe Gin GI y Ala 435 Giu Le u Ile Gi y Ala Aia Val1 Leu Arg 260 Gly Phe Giu Gly Phe 340 Ala Arg Asp Ala Met 420 Ph e Gi u Asp As n Giy 500 Al a Arg Val Val1 245 Le u Al a Le u Thr His 325 Glu Pro Met Se r Le u 405 Pro Val1 Val1 Giu Giy 485 Th r Phe His Al a 230 Giu His Gi y G lu Th r 310 Giu Phe Gly Asp Met 390 Gin Thr Gly T rp Asp 470 Arg Ala Gly Val1 215 Gly Giu Se r Th r Val1 295 Giy Leu Arq Lys Se r 375 Le u Arg Val1 As n Asp 455 Giu Ar g Gly Arg 200 Gi u Thr Al a Se r Vali 280 Asn Ile Se r Ile Ile 360 Gi y Al a Ser Ile Asp 440 As n Asp Val1 Pro Gly Al a Arg Pro Ala 265 Glu Thr Asp Ile Asn 345 Thr Val Lys Arg Pro 425 Glu Pro Lys Giu Lys 505 Ph e Ile 220 Se r Phe Ile ValI Gin 300 Arg Asp Asp Arg Giy 380 Val1 Le u Gin Giu Pro 460 Aia Le u Ala Val 205 Ala Le u Le u Cys Gly 285 Val Giu Pro Aia Glu 365 Ser Trp Al a His Ile 445 Tyr Gin Pro Lys Giu Asp Gin Thr Lys 270 Se r Glu Met Ala Gly 350 Pro Giu Giy Giu Ile 430 Ty r ValI Lys Giy Lys 510 Arq Lys Arg Asp 255 Giu Asp His Phe Pro 335 Se r Gin Ile Asp T yr 415 ValI Th r Asp Vali Asp 495 Arq Tyr His Arg 240 Asp Ala Gly Pro Arg 320 Arg As n Gly Ser Th r Val Giu Lys Aia Val 480 Leu Arg Al a Met Asn 545 Pro Ala WO 01/00805 Gly Gly Ala 515 Gin Gly Thr 530 Glu Gly Asp Val Lys Ala Gly Glu Gly 580 PCT[IBOO/00926 Lys Ala Gly Val 520 Val Ile Lys Val 535 Thr Val Val Val 550 His Lys Ser Gly 565 Val Asn Lys Gly Ser Gly Asp Ala Vai 525 Asn Vai Giu Giu Giy 540 Leu Glu Ala Met Lys 555 Thr Val Thr Giy Leu 570 Val Vai Leu Leu Giu 585 Ala Ala Pro Ala Giu Val Met Giu Asn 560 Thr Vai Ala 575 Ile Lys 590 (210> <211> 1575 '212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1552) <223> RXA02173 <400> ctaaattgqg cttagatctt ccqcctctaa atagqtatgc aga acaaagccat ttttcggccg tggaqaagcg ttttccgact atq Met gacattc gaattaatta gtq tgg ggc atg Val Trp Gly Met gaa Glu agc Se r gg C GI y gaa Gi u qtt Val1 gcg Aila gca Al a tqg Trp acc Th r act Thr ga t Asp atc Ile t tq Leu aat As n t tg Leu gga Gly ttt Phe 75 atg Met a cg Thr ga a Glu gag Gi u gaa G iu tcc Ser aaq Lys gac Asp caa Gin cga Arg gtq Val1 Gly cac His 1 gtt ValI qac Asp aag Lys ggc Gly t Ct Ser gcc Ala t tg Leu aac As n gcc Ala att Ile ttg Leu aca Thr ga C Asp ctc Le u aaa Lys ccg Pro ggc Gi y gag Gi u gac Asp caa Gin ga t Asp gcc Ala gtC ValI aaa Lys ctc cca ctg ctg gtc tcc cct gct tcc ggt ggt gcg cgc atq cag gaa Leu Pro Leu Leu Val Ser Pro Ala Ser Gly Gly Ala Arg Met Gin Giu WO 01/00805 gac aat cga gct Asp Asn Arq Ala 120 cgt cac cgc gag Arg His Arg Giu 135 acg atg ggt qgc Thr Met Gly Gly 150 ttt qcq gaa ccc Phe Ala Glu Pro gag tta acc act Glu Leu Thr Thr 185 aat ttg gtg aaa Asn Leu Val Lys 200 ttg cgt qca gcg Leu Arg Ala Ala 215 qca acg gat cgt Ala Thr Asp Arg 230 atq gag gcg att Met Glu Ala Ile gag att atg gaa Glu Ile Met Glu 265 cgt gct ggc gca Arg Ala Gly Ala 280 ggg ggc cgg ccc Gly Gly Arg Pro 295 ggg ccg cag gag Gly Pro Gin Glu 310 gag cta aac ctg Glu Leu Asn Leu ttg tcg cag gcg Leu Ser Gin Ala 345 acc ttg tcc aag gtC Val1 cat His atg Met 155 gcg Ala cat His gg t Gly g ca Al a tct Se r 235 cgt Arg ttg Leu agc Se r gtg Val1 cg t Arg 315 atc Ile gag Giu atc atg Met t tg Leu 140 gcc Ala cag Gin gcg Al a g tg Val1 aaa Lys 220 cca Pro tct Se r ggg Gly ccg Pro ctg Le u 300 ttt Phe gtg Val gag Giu ga c a cc Thr tat Tyr 145 tct Se r ggt Gly gtg Val1 gtg Val1 att Ile 225 gtg Val1 agg Arg aag Ly s gcc Ala cgc Arg 305 att Ile acc Thr agc Se r acc gct Al a cgc Arg cat His cg c Arg cag Gin 195 cca Pro c cg Pro ctt Leu gga Gly tct Se r 275 gcg Ala ttc Phe ctg Leu ggc Gi y att Ile 355 tcg g tg Val1 aat As n ctc Leu gtg ValI 180 g cg Ala ctc Le u gta Vai ccg Pro atc Ile 260 ggt Gly cgc Arg acg Th r gcg Al a gcc Ala 340 g cg Ala gtc PCT/IBOO/00926 cag 499 Gin ccc 547 Pro act 595 Thr 165 gtg 643 Val1 gag 691 Gi u caa 739 Gin gag 787 Giu gtg 835 Vai 245 ggg 883 Gly gcg 931 Ala atc 979 Ile ctt 1027 Le u cgc 1075 Arg 325 gaa 1123 Glu cgc 1171 Arg att 1219 wo 01/00805 WO 0100805PCTJIBOO/00926 Thr Leu Ser Lys Leu Ile Asp Ala Pro Leu Pro ggt Gly 375 gtc Val1 gcc Al a gag Gi u gac Asp aca Th r 455 gg a 360 ca g Gin tac Tyr t cg Ser cg a Arg ggg Gly 440 atc Ile cgc gg C Gi y gcg Al a g cc Al a caa Gin 425 atc Ile agc Ser ga c gtt Val1 gcc Ala atc Ile 410 99c Gly gtc ValI aa c As n agt gg C Gly gaa Glu 395 ctc Leu gtg Val gcc Al a g cc Ala cgc Arg 475 ggt Gly 380 aa c As n ttc Ph e ca g Gin gaa Giu ctc Leu 460 ttc 365 ggc Gly g cg Ala cgc Arg gcg Al a a cc Th r 445 tcc Ser g cg Al a tgg Trp gac Asp ca c His 430 gag Giu gaa Glu Ile gat Asp gag Glu 405 atc Ile ctt Le u ctc Leu agg Arg 1267 1315 1363 1411 1459 1507 1552 1575 aca cga ttt gag cgt tta gcg cag Ala Giy.Arg Asp Ser 470 Phe Thr Arg Phe Giu Arg. Leu Ala Gin 480 taaagaaaat tatgcgctga tca <210> 26 <211> 484 <212> PRT <213> Corynebacterium glutamicum <400> 26 Met Val Trp Gly Met Glu His Thr Ser 1 5 Val Leu Asp Pro Asp Ser Phe Ile Ser Asp Asn Leu Asn Gin Gly Tyr Ala Glu Lys Ala Lys Cys Asp Giu Ser Val Ilie Gly Ile Pro Val Ala Val Ile Leu Ser 70 Ser Leu Gly Thr Val Ala Ser Val Arg Ala Thr Glu Leu Lys Leu Pro Leu Leu 100 105 Asp Gin Arg Val Gly His Gly Ser Tyr Ser Giu Gly Arg Gly WO 01/00805 PCT/IB00/00926 Ala Arg Met Gin Glu Asp Asn Arg Ala Phe Val Met Met Val Ser Ile 115 120 125 Thr Ala Ala Val Gin Arg His Arg Glu Ala His Leu Pro Phe Leu Val 130 135 140 Tyr Leu Arg Asn Pro Thr Met Gly Gly Ala Met Ala Ser Trp Gly Ser 145 150 155 160 Ser Gly His Leu Thr Phe Ala Glu Pro Gly Ala Gin Ile Gly Phe Leu 165 170 175 Gly Pro Arg Val Val Glu Leu Thr Thr Gly His Ala Leu Pro Asp Gly 180 185 190 Val Gin Gin Ala Glu Asn Leu Val Lys Thr Gly Val Ile Asp Gly Ile 195 200 205 Val Ser Pro Leu Gin Leu Arg Ala Ala Val Ala Lys Thr Leu Lys Val 210 215 220 Ile Gin Pro Val Glu Ala Thr Asp Arg Phe Ser Pro Thr Thr Pro Gly 225 230 235 240 Val Ala Leu Pro Val Met Glu Ala Ile Ala Arg Ser Arg Asp Pro Gin 245 250 255 Arg Pro Gly Ile Gly Glu Ile Met Glu Thr Leu Gly Ala Asp Val Val 260 265 270 Lys Leu Ser Gly Ala Arg Ala Gly Ala Leu Ser Pro Ala Val Arg Val 275 280 285 Ala Leu Ala Arg Ile Gly Gly Arg Pro Val Val Leu Ile Gly Gin Asp 290 295 300 Arg Arg Phe Thr Leu Gly Pro Gin Glu Leu Arg Phe Ala Arg Arg Gly 305 310 315 320 Ile Ser Leu Ala Arg Glu Leu Asn Leu Pro Ile Val Ser Ile Ile Asp 325 330 335 Thr Ser Gly Ala Glu Leu Ser Gin Ala Ala Glu Glu Leu Gly Ile Ala 340 345 350 Ser Ser Ile Ala Arg Thr Leu Ser Lys Leu Ile Asp Ala Pro Leu Pro 355 360 365 Thr Val Ser Val Ile Ile Gly Gin Gly Val Gly Gly Gly Ala Leu Ala 370 375 380 Met Leu Pro Ala Asp Leu Val Tyr Ala Ala Glu Asn Ala Trp Leu Ser 385 390 395 400 Ala Leu Pro Pro Glu Gly Ala Ser Ala Ile Leu Phe Arg Asp Thr Asn 405 410 415 His Ala Ala Glu Ile Ile Glu Arg Gin Gly Val Gin Ala His Ala Leu 420 425 430 Leu Ser Gin Gly Leu Ile Asp Gly Ile Val Ala Glu Thr Glu His Phe WO 01/00805 PCT/EB00100926 435 440 445 Giu Glu Ile Leu Gly Thr Ile Ser Asn Ala Leu Ser Glu Leu Asp 450 455 460 Asn Pro Giu Arg Ala Gly Arg Asp Ser Arg Phe Thr Arg Phe Giu 470 475 480 Leu Ala Gin <210> 27 <211> 1056 <212> DNA <213> Corynebacterlwn glutamicum <220> <221> COS <222> (1033) <223> RXA01764 <400> 27 ttttcaaaat taatagttga cattttcaac gttatgagtt acgaagtgtc tgggatcaca aaccttcaaa ggagtttgaa ttcattggta tcacgccccg tct ctc aat Ser Leu Asn aaa Lys gc t Al a aac Asn ggc Gl y gca Ala ctt Le u a cg Thr gt c Val ca g Gin cag Gin aa a Lys ttg Leu gtc Val1 gac Asp qcc Ala gag Glu g ca Ala gcc Al a ctg Leu aca Thr gat Asp gg a Gly cag Gin gag Glu atc Ile gcc Ala atc Ile gca Al a t ct Ser ggt Gly act Thr gcc Al a gtc Val1 ctt Leu gtc Val1 cgt Arg aa t Asn ga a Giu gaa Glu ttg Leu ct g Leu aag Lys 115 tcc Ser gac Asp gcc Al a agc Ser gac Asp aag Lys 100 gg C Gly gga Gly ttc Phe gt a Val ggC Gly g cc Al a att Ile atg Met act Thr ttc acc tgt gtt cgc gag gca ttt gga tac ttc aag gag aat gga atc Phe Thr Cys 120 Val Arg Giu Ala Phe Gly Tyr Phe Lys Giu Asn Gly Ile WO 01/00805 gcg ggg cgc atc gtc: acg att ggt PCT/IB00100926 tct ccc acc ggg cag cgc ggc: aac 547 Giy 135 gga Giy cg c Arg atc Ile cag Gin ttc Ph e 215 gtg Vali atc Ile g ca Aila gaa Giu gtt Arg ca g Gin a cg Th r att Ile aag Ly s 200 cg c Arg gcc Aia gq t Giy gtt Vali cgt Arg 280 ttC Ilie Vai agc aat Ser Asn tgg gcg Trp Aia 170 ccg gaa Pro Giu 185 gct gta Ala Vai gag acc: Giu Thr ttc ctt Phe Leu gca ggc Aia Gly 250 act gaa Thr Giu 265 ggc aag Giy Lys cct gaa Thr Ilie Gly Ser Pro Thr 140 Gin Arg Giy Asn gct Aia aag Ly s ga t Asp 190 gag Giu Gly gag Giu atg Met ggt Giy 270 gag Giu gag aag Lys cgc Arg 175 a tg Met cg t Arg act Th r gca Aia cag Gin 255 ggc Giy Gi y ctt Le u a tg Met 165 aac Asri tat Tyr gca Al a gga Gly ca g Gin 245 gag Giu ctg Leu ggt Gly gt C Val1 595 643 691 739 787 835 883 931 979 1027 1056 Val Vai Phe Pro Giu Leu Pro Ala Giu 295 300 aag qca taacaccgca Caccaagaat ttt Lys Ala 310 <210> 28 <211> 311 <212> PRT <213> Corynebacteriun giutamicun <400> 28 Met Ser Leu Asn Gly Lys Vai Ala Ile Vai Thr Giy Ser Giy Ala Giy 1 5 10 Leu Giy Arg Ser Phe Ala Gin Giu Leu Ala Arg Gin Gly Ala Ser Val 25 WO 01/00805 WO 0100805PCTIBOO/00926 Ile Val Asn Asp Val Asn Gin Ala Ala Ala 3 Ile Thr G1i Pro Ser Gi Gly Ser Lei Ser Leu Lei His Leu Ly: 11 Lys Glu Asi 130 Giy Gin Ar 145 Gly Ile Va.

Gly Val Th: Lys Thr Va.

19 Glu Ala Mel 210 Gin Asp Va.

225 Asn Ilie Se: Trp Lys Hi: Thr Tyr Gli 27! Leu Gin Se: 290 Pro Gin Il 305 <210> 29 <211> 1827 <212> DNA Ala Se r Asp Ly s 100 Gly Gl y Gly Gly Ile 180 Pro Pro Al a Gi y Pro 260 Ala Val1 Pro Gly Ala Ile Met Thr le Asn Met 165 Asn Tyr Al a Gly Gin 245 Gi u Le u Gly ValI Ala Val1 Asn Asp 105 Val1 Ile Ser Trp Pro 185 Ala Giu Ph e Al a Th r 265 Giy Pro Ala Arg Al a 90 Ph e Arg Val1 As n Ala 170 Glu Val1 Th r Leu Gi y 250 Glu Lys Glu Asp Val Glu 75 Gly Asp Gi u Th r Tyr 155 Leu Al a Gi u Le u Ser 235 GI y Ph e Asn Le u Gi u Ile Al a Ile Ala Ala Ile 140 Al a Giu Ala Ala Gly 220 Ser Asp Asn Ile Pro 300 Al a Val1 Lys Asp Asn Tyr Pro Ly s Arg 175 Met Arg Thr Ala Gin 255 Gly Gly Leu Ala Gly Phe Arg Val1 Phe Thr Ala 160 Al a Th r Gly Pro Al a 240 Val1 Trp Asn Gin <213> Corynebacterium glutamicum <220> WO 01/00805 <221> CDS <222> (1804) <223> RXN02487 <400> 29 tcatagctac gcgcatgccc acattctaga tcgccgaaga PCTIBOO/00926 aagcagcggg acgtctctat atactaaagg gcactaaagc aacgcagttg aagggacacc atg tca gca tac gaa Met Ser Ala Tyr Giu acc Th r gaa Glu att Ile t ac Tyr q ca Ala t gc Cys gta Val ccc Pro tcc Ser g tg Val1 150 ctt Leu ggc Gly aaa Lys tat Tyr aac Asn ggt Gly cta Le u cca Pro gt c ValI ttc Phe ccc Pro 135 tcg Ser cg g Arg gc a Al a gaa Glu 9gC Gly gca Al a gaa Glu gg t Gly cag Gin att Ile aa a Lys 120 acc Th r gtc Val ctc Leu gCC Ala tgg Trp gac Asp gac Asp ct g Leu gtC Val1 cac His gag Giu 105 gac Asp gtc Val1 aac Asn cca Pro ccc Pro 185 ctc Leu a cc Thr aaq Lys ga c Asp cgc Arg atc Ile cac His cac His ga a Giu atg Met atc Ile 170 aac Asn cag Gin acc Thr cca Pro aaa L ys ccc Pro 75 g ca Ala aac Asn ggt Gi y cag Gin atc Ile 155.

cct Pro aCC Th r cac His ctg Leu g cc Al a gaa Giu 60 ggC Gly gct Al a ccg Pro gcc Al a cta Leu 140 aac As n gca Ala gt t Val tac Tyr ctg Leu acc Thr 45 gt C Val1 gat Asp ttc Ph e ctc Leu cgc Arg 125 cg t Arg gcg Al a ctg Le u c t Pro 1 cca gag tgg acg cca cac tcg ctg Pro Giu Trp Thr Pro His Ser Leu 15 gac gtt tac gac aac aac ctg gcc Asp Val Tyr Asp Asn Asn Leu Ala 30 tac ttt ttc ggt cgt tca caa acc Tyr Phe Phe Gly Arg Ser Gin Thr cgc aaa act gcc gct ggc ctg cgc Arg Lys Thr Ala Ala Gly Leu Arg cac gta gcg att atc ctc ccc aac His Val Ala Ile Ile Leu Pro Asn 80 tac gca gtg ctg aaa ctc ggc gca Tyr Ala Val Leu Lys Leu Gly Ala 95 100 tac acc gcc cac gaa ctg ctc gaa Tyr Thr Ala His Giu Leu Leu Giu 110 115 gtt gcc atc gtc tgg gac aaa gcc Val Ala Ile Val Trp Asp Lys Ala 130 gga cag acc cag ttg gaa acc atc Gly Gin Thr Gin Leu Giu Thr Ile 145 atg cca cca ctc cag cgc cta gca Met Pro Pro Leu Gin Arg Leu Ala 160 165 cgc aag agc cgc gaa tcc ctc tcc Arg Lys Ser Arg Giu Ser Leu Ser 175 180 ttt gaa acc ctg acc agc gca gca Phe Glu Thr Leu Thr Ser Ala Ala 190 195 gtt tca gaa ccc acc gtg acc aaa Val Ser Giu Pro Thr Val Thr Lys atg ggc ggc gac qgc gac gac gta Met Gly Gly Asp Gly Asp Asp Val WO 01/00805 WO 0100805PCTIBOOIOO926 gaa Glu aeg Lys 230 gga Gly g ca Ala ctg Le u etc Ilie cca Pro 310 gaa Giu ace Thr cqc Arg ggt Gly ttc Phe 390 a cc Thr gtg Val1 cec His tcc Ser 215 ggt Gly ag Ly s gcc Al a tcc Se r gec Asp 295 gg c Gly gge Gly ctc Le u ctc Le u eec Asn 375 ccc Pro atg Met ttc Ph e ggc Gly gtc Val1 g cc Ala ca c His cta Leu gtg Val1 280 ctg Leu gtg Vel etc Ile tcc S er gtc Vea1 360 ccc Pro gec Asp ccc Pro aag Lys gag Glu 440 gcg Ala ca g Gin tgg Trp cce Pro 265 ttc Phe atc Ile ccc Pro ccc Pro ceg Gin 345 gee Glu atg Met acc Thr gec Asp gg t Gly 425 t gg Trp ct g Le u ctc Leu gt t Val1 250 atg Met etc Ile atg Met ecc Th r etc Ile 330 cgc Arg ggc Gly egc Se r etc Ile Gly 410 tec T yr tec T yr etc Ile ecc Thr 235 cca Pro ttc Ph e ggt Gly eec Asn ctt Leu 315 a eq Lys a cc Thr tac Tyr get Asp gtg Val1 395 egc Ser ct c Leu cg c Arg ctc Leu 220 cec His gg t Gly cec His gg c Gly gte Val1 300 tec Ty r gg a Gly gtt ValI Gly cec His 380 cg c Arg gee Glu eec Asn ecc Th r tac Tyr gge Gly ctc Leu g ce Ale gee Glu 285 atg Met gee Giu gtc Val1 gaa Glu ctc Le u 365 cge Arg etc Ile Gly ceg Gin Gly 445 acc Th r eec Asn gga Gi y tec Tyr 270 etg Met eee Lys eee Lys cgc Ar g cg c Arg 350 acc Th r cgc Arg gca Ale gee Giu gee Gi u 430 ga c Asp tcc Ser ctg Le u gac Asp 255 gg t Gi y gt g Vel eag Lys etc Ile eec Asn 335 tgg Trp gee Glu cee Gin eec Asn gtc Val1 415 gee Glu gtc Val g gc Gly ttc Phe 240 aee Lys ctg Le u cta Le u cac His gt c Vel 320 gcc Ale gee Gi u ecc Thr gg c Gi y cce Pro 400 cte Leu gcc Ale gg a Gly acc ecc Thr Thr 225 ttc eat Phe Asn cce gee Pro Glu ecc atg Thr Met ctt ccc Leu Pro 290 acc cce Thr Pro 305 gec gcc Asp Ala ttc tcc Phe Ser aeg cac Lys His tcc ccc Ser Pro 370 tec gte Tyr Val 385 gee aec Glu Asn gtc aeg Val Lys ecc eag Thr Lys gtg atg Val Met 450 gge Gly ctt Leu cg c Arg gtc Val1 275 acc Thr ecc Thr tcc Ser ggt Gly ecc Thr 355 etc Ile gge Gly ct c Leu ggc Gly eec Asn 435 gee Glu cgc Arg ctt Leu etg Met 260 ggc Gi y cce Pro t gg Trp gee Glu gce Ale 340 Gi y etc Ile etc Ile gec Asp cce Pro 420 eq c Se r gee Glu ccc Pro ceaa Gin 245 ctt Le u ace Thr cg c Ar g cte Leu eee Lys 325 tcc Se r gge Gly gtg Val1 ccc Pro gee Glu 405 ceg Gin ttc Phe ge c Asp 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 wo 01/00805 WO 0100805PCT/IBOO/00926 ggg Gly gg t Gly 470 cca Pro tcc Ser gat Asp a ag Lys atg Met 550 ct C Le u atc cgc cta gtt Ile Arg Leu Val aac gtg tac cca Asn Val Tyr Pro 475 att gaa gat tcc Ile Glu Asp Ser 490 aac gtc gtt gct Asn Val Val Ala 505 gat ggc ctg aag Asp Gly Leu Lys 520 ccg cgc act ttc Pro Arg Thr Phe aag att aqg cgt Lys Ile Arg Arg 555 aag tagacgccga Lys gct Al a 460 gct Al a go a Al a gcc Al a gaa Glu tac Tyr 540 cg t cgc Arg gag Glu gtc Val atc Ile ttC Phe 525 cac His g aa atc Ile ctc Le u cgt Arg gg t Gly ctc Le u 530 cg Pro ttg act Th r gag Giu gac Asp 500 gcg Ala cg c Arg ga t Asp a ag ggC Gly cac His 485 gg C Gi y ctg Leu tac Tyr ca g Gin aag 1507 1555 1603 1651 1699 1747 1795 1827 Arg Glu Val Gin Ala Glu Leu Leu Lys tttaagaggt cga <210> <211> 568 <212> PRT <213> Corynebacterium glutamicum <400> Met Ser Ala Tyr Glu Thr Lys Giu Trp 1 5 Thr Pro His Ser Leu Glu Tyr Gly Asp Asp Asn Asn Leu Ala Ile Asn Ala Asp 40 Gly Arg Ser Gin Thr Tyr Gly Giu Leu 55 Ala Ala Gly Leu Arg Ala Leu Gly Val 70 Ile Ile Leu Pro Asn Cys Pro Gin His Leu Lys Leu Gly Ala Val Val Ile Glu 100 105 Gin Th r Pro Lys Pro 75 Al a Asn His Le u Al a Giu Gly Ala Pro Tyr Leu Thr Val1 Asp Ph e Le u Glu Val Ph e Ly s Val Ala Thr Trp Tyr Phe Thr Al a Val1 Ala WO 01/00805 WO 0100805PCT/IBOO/00926 His Giu Leu Leu Glu Pro Phe Lys Asp His Gly Ala Val1 Gin 145 Leu Arg L eu Pro Thr 225 Phe Pro Th r Leu Thr 305 Asp Ph e Lys Ser Tyr 385 Glu ValI Thr Trp 130 Leu Gin Glu Thr Thr 210 Thr As n Glu Met Pro 290 Pro Al a Ser His Pro 370 Val1 Asn Lys Lys 115 Asp Lys Glu Thr Arg Leu Ser Leu 180 Ser Ala 195 Val Thr Gly Arg Leu Leu Arg Met 260 Val Gly 275 Thr Pro Thr Trp Ser Glu Giy Ala 340 Thr Gly 355 Ile Ilie Gly Ilie Leu Asp Gly Pro 420 Asn Ser 120 Al a Ilie Al a 165 Ser Al a Lys Pro Gin 245 Leu Th r Arq Le u Lys 325 Ser Giy Val1 Pro Glu 405 Gin Ph e Ser Val1 150 Le u Gly Met Glu Lys 230 Gly Al a Le u Ilie Pro 310 Glu Th r Ar g Giy Ph e 390 Th r Val1 His Pro 135 Se r Arg Ala Gly Ser 215 Gly Lys Ala Ser Asp 295 Gly Gly Leu Leu As n 375 Pro Met Ph e Gly Thr Val Leu Al a Gly 200 Val Ala His Le u Val1 280 Leu Val1 Ile Ser Val1 360 Pro Asp Pro Lys Glu Le u 140 Asn Al a Val1 Asp Leu 220 His Gly His Gly Val 300 Tyr Gi y Val Gly His 380 Arg Glu As n Thr Arg 125 Arg Ala Le u Pro Vai 205 Tyr Gl y Leu Al a Giu 285 Met Giu Val1 Giu Leu 365 Arg Ile Gly Gin Gly Val Ala Ile Gly Met Arg Phe 190 Val Thr Asn Gly Tyr 270 Met Lys Lys Arg Arg 350 Thr Arg Al a Giu Giu 430 Asp Gin Pro Lys 175 Giu Ser Ser Leu Asp 255 Gi y Val L ys Ile Asn 335 Trp Giu Gin Asn Val1 415 Glu Val WO 01/00805 435 Val Met Glu GI 450 Val Ile Ile Ti' 4 65 Val Leu Ala Gi Pro Arg Glu As Glu Gly Ala Al 515 Asn Leu Thr Ar 530 Met Pro Arg As 545 Glu Leu Leu LV PCT~iBOOIOO926 Asp Gly His 485 Gly Leu Tyr Gln Ly s 565 Gly Gly 470 Pro Ser Asp Lys Met 550 L eu Phe 455 Ph e Asp Glu Pro Val 535 Gly Gly 445 Arg Glu Val1 Ile Phe 525 His Glu Ile Val Val Thr 510 Ala Ph e ValI <210> 31 <211> 411 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (388) <223> FRXA02487 <400> 31 ggcgagtggt accgcaccgc gacgtcggag tgatgaagaa tgctcgcatc aagaaagtca tcatcactgg cggtttcaac gtt Val gtt Val act Thr g cc Ala ttt ga a Glu ggt Gly ttg Leu cg c Arg gag gtc Val1 ccg Pro ga a Glu aa c Asn atg gag Glu ga c Asp g cg Ala cgc Arg 60 ga t gccgggttca tccgcctagt gtg tac: cca gct gag Val Tyr Pro Ala Glu 1 gaa gat tcc gca gtC Glu Asp Ser Ala Val gtc gtt gct gcc atc Val Val Ala Ala Ile ggc ctg aag gaa ttc Gly Leu Lys Glu Phe cgc act ttc tac cac Arg Thr Phe Tyr His att agg cgt cgt gaa WO 01/00805 PCTJIEBOO/00926 Phe Glu Glu Met Pro Arg Asp Gin Met Gly Lys Ile Arg Arg Arg Glu 75 80 gtg cag gcg gag ttg ttg aag aag ctc gqc aag tagacgccga tttaagaggt 408 Val Gin Ala Giu Leu Leu Lys Lys Leu Gly Lys cga 411 <210> 32 <211> 96 <212> PRT <213> Corynebacterium glutamicum <400> 32 Val Tyr Pro Ala Giu Vai Glu Glu Val Leu Ala Giu His Pro Asp Ile 1 5 10 Glu Asp Ser Ala Val Val Gly Ile Pro Arg Giu Asp Gly Ser Giu Asn 25 Val Vai Ala Ala Ile Thr Leu Val Giu Gly Ala Ala Leu Asp Pro Asp 40 Gly Leu Lys Giu Phe Ala Arg Lys Asn Leu Thr Arg Tyr Lys Val Pro 55 Arg Thr Phe Tyr His Phe Glu Glu Met Pro Arg Asp Gin Met Gly Lys 70 75 Ile Arg Arg Arg Glu Vai Gin Ala Glu Leu Leu Lys Lys Leu Gly Lys 90 <210> 33 <211> 913 <212> DNA <213> Corynebacterium glutanicum <220> <221> CDS <222> .(101)..(913) <223> FRXA02490 <400> 33 tcatagctac gcgcatgccc acattctaga tcgccgaaga aagcagcggg acgtctctat atactaaagg gcactaaagc aacgcagttg aagggacacc atg tca gca tac gaa 115 Met Ser Ala Tyr Giu 1 acc aaa gaa tgg ctc cag cac tac cca gag tgg acg cca c *ac tcg ctg 163 Thr Lys Glu Trp Leu Gin His Tyr Pro Glu Trp Thr Pro His Ser Leu 15 gaa tat ggc gac acc acc ctg ctg gac gtt tac gac aac aac ctg gcc 211 Giu Tyr Gly Asp Thr Thr Leu Leu Asp Val Tyr Asp Asn Asn Leu Ala WO 01/00805 WO 0100805PCTJIBOOIOO926 att Ile tac Tyr g ca Ala tgc C ys gt a Val1 ccc Pro tcc Ser gtg Val 150 ctt Leu gg C Gly atg Met gaa Glu a ag Lys 230 qqa Gly gca Ala ga c Asp ctg Leu gtc Val1 cac His gag Glu 105 ga c Asp gtc Val1 aac Asn cca Pro ccc Pro 185 ga c Asp g cg Al a cag Gin t gg Trp cca Pro 265 aag Lys ga c Asp cg c Arg atc Ile cac His cac His gaa Gi u atg Met atc Ile 170 aac Asn ggC Gly Ct 9 Leu ct C Le u gtt Val1 250 a tg Met tac ttt ttc ggt cgt tca Tyr Phe Phe Gly Arg Ser cgc aaa act gcc gct ggc Arg Lys Thr Ala Ala Gly cac gta gcg att atc ctc His Val Ala Ile Ile Leu tac gca gtg ctg aaa ctc Tyr Ala Val Leu Lys Leu tac acc gcc cac gaa ctg Tyr Thr Ala His Glu Leu 110 115 gtt gCC atc gtc tgg gac Val Ala Ile Val Trp Asp 130 gga cag acc cag ttg gaa Gly Gin Thr Gin Leu Glu 145 atg cca cca ctc cag cgc Met Pro Pro Leu Gln Arg 160 cgc aag agc cgc gaa tcc Arg Lys Ser Arg Glu Ser 175 ttt gaa acc ctg acc agc Phe Giu Thr Leu Thr Ser 190 195 gtt tca gaa ccc acc gtg Val Ser Glu Pro Thr Val 210 acc tcc gqc acc acc gga Thr Ser Gly Thr Thr Gly 225 aac ctg ttc tcc aat ctC Asn Leu Phe Ser Asn Leu 240 gga gac aaa cca gaa c9c Gly Asp Lys Pro Glu Arg 255 tac ggt Tyr Gly 270 WO 01/00805 PCT/IB00/00926 <210> 34 <211> 271 <212> PRT <213> Corynebacterium glutamicum <400> 34 Met Ser Ala Tyr Glu Thr Lys Glu Trp Leu Gin His Tyr Pro Glu Trp 1 5 10 Thr Pro His Ser Leu Glu Tyr Gly Asp Thr Thr Leu Leu Asp Val Tyr 25 Asp Asn Asn Leu Ala Ile Asn Ala Asp Lys Pro Ala Thr Tyr Phe Phe 40 Gly Arg Ser Gin Thr Tyr Gly Glu Leu Asp Lys Glu Val Arg Lys Thr 55 Ala Ala Gly Leu Arg Ala Leu Gly Val Arg Pro Gly Asp His Val Ala 70 75 Ile Ile Leu Pro Asn Cys Pro Gin His Ile Ala Ala Phe Tyr Ala Val 90 Leu Lys Leu Gly Ala Val Val Ile Glu His Asn Pro Leu Tyr Thr Ala 100 105 110 His Glu Leu Leu Glu Pro Phe Lys Asp His Gly Ala Arg Val Ala lie 115 120 125 Val Trp Asp Lys Ala Ser Pro Thr Val Glu Gin Leu Arg Gly Gin Thr 130 135 140 Gin Leu Glu Thr Ile Val Ser Val Asn Met Ile Asn Ala Met Pro Pro 145 150 155 160 Leu Gin Arg Leu Ala Leu Arg Leu Pro Ile Pro Ala Leu Arg Lys Ser 165 170 175 Arg Glu Ser Leu Ser Gly Ala Ala Pro Asn Thr Val Pro Phe Glu Thr 180 185 190 Leu Thr Ser Ala Ala Met Gly Gly Asp Gly Asp Asp Val Val Ser Glu 195 200 205 Pro Thr Val Thr Lys Glu Ser Val Ala Leu Ile Leu Tyr Thr Ser Gly 210 215 220 Thr Thr Gly Arg Pro Lys Gly Ala Gin Leu Thr His Gly Asn Leu Phe 225 230 235 240 Ser Asn Leu Leu Gin Gly Lys His Trp Val Pro Gly Leu Gly Asp Lys 245 250 255 Pro Glu Arg Met Leu Ala Ala Leu Pro Met Phe His Ala Tyr Gly 260 265 270 <210> WO 01/00805 <211> 414 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (391) <223> RXA01467 <400> gcgtcatctt tagccttgct ttatgagctt ttccccgcaa taagtcttag cctacaagcg ctttgaggta gtttgggatc PCTJIBOO/00926 tgagccgcgt tgtgtatcca atg gat ttc aac gac Met Asp Phe Asn Asp 1 aaa gcc gct tca gaa aac gct gta aag act ggc gca gaa ggc ccc aac Lys Ala Ala Ser Glu Asn Ala Val Lys Thr Gly Ala Glu Gly Pro Asn 15 gtt ttc gcg agc gtg goc aag att ttg cag gat gtt ggc gga att tca Val Phe Ala Ser Val Ala Lys Ile Leu Gln Asp Val Gly Gly Ile Ser 30 gcc gaa gac gtc act ccg gaa tct cgt ttt act gag gat ttg gca gtg Ala Glu Asp Val Thr Pro Glu Ser Arg Phe Thr Glu Asp Leu Ala Val 45 agc tca ctc aat tac atc gag ttg att gtc aat gcg gag gac gca ttt Ser Ser Leu Asn Tyr Ile Glu Leu Ile Val Asn Ala Glu Asp Ala Phe 60 ggt gtt cgc att gag gac gcc gat gcc aag gat ttg acc acc gtg cag Gly Val Arg Ile Glu Asp Ala Asp Ala Lys Asp Leu Thr Thr Val Gln 75 80 gat ttg att gac ttt att aac acc aat aag gct gat tagcgggaaa Asp Leu Ile Asp Phe Ile Asn Thr Asn Lys Ala Asp atttcgccca aaa <210> 36 <211> 97 <212> PRT <213> Corynebacterium glutamicum <400> 36 Met Asp Phe Asn Asp Lys Ala Ala Ser Glu Asn Ala Val Lys Thr Gly 1 5 10 Ala Glu Gly Pro Asn Val Phe Ala Ser Val Ala Lys Ile Leu Gln Asp 25 Val Gly Gly Ile Ser Ala Glu Asp Val Thr Pro Glu Ser Arg Phe Thr 40 Glu Asp Leu Ala Val Ser Ser Leu Asn Tyr Ile Glu Leu Ile Val Asn 55 WO 01/00805 PCTJIBOO/00926 Ala Glu Asp Ala Phe Gly Val Arg Ile Glu Asp Ala Asp Ala Lys Asp 70 75 Leu Thr Thr Val*Gln Asp Leu Ile Asp Phe Ile Asn Thr Asn Lys Ala 90 Asp <210> 37 <211> 298 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (298) <223> RXA00796 <400> 37 ctggaatccc aaagcgcttg catqacgcgg aaagttaaca gttgatacat caacttaatt aacttttaga qqagtacacc atc atc acc ggt tcc acc cgt cca ggc Ile Ile Thr Gly Ser Thr Arg Pro Giy aac tgg gtt ctc gag cgc gca caa gag Asn Trp Val Leu Glu Arg Ala Gin Giu 30 ctc gtt gat atc gcc gat ttc aac ttc Leu Vai Asp Ile Ala Asp Phe Asn Phe 45 cca gcc gqc tac ggc cag tat gca aac Pro Ala Giy Tyr Giy Gin Tyr Ala Asn 60 <210> 38 <211> 66 <212> PRT <213> Corynebacterium qiutamicui <400> 38 Met Ser Lys Ile Ala Ile Ile Thr Gly 1 5 Asn Ile Asp Val Ala Asn Trp Vai Leu 25 Asp Ala Gin Tyr Giu Leu Val Asp Ile 40 Leu Asp Giu Ala Met Pro Ala Gly Tyr 55 taggctaggt acgtcaagtt atg agc aag atc gcc Met Ser Lys Ile Ala 1 aac att gac gta gcc Asn Ile Asp Vai Ala gat qca cag tac gag Asp Ala Gin Tyr Giu ctc gac gaa gca atg Leu Asp Giu Ala Met acc aag Thr Lys Ser Thr Arg Pro Gly Arg Val 10 Giu Arg Ala Gin Giu Arg Asn Ala Asp Phe Asn Phe Pro Val Giy Gin Tyr Ala Asn Giu His WO 01/00805 WO 0100805PCT/IB00100926 Thr Lys <210> 39 <211> 666 <212> DNA <213> Corynebacterium <220> <221> CDS <222> (101)..(643) <223> RXA01897 <400> 39 etatttagat cgttccggaa giutamicum ecgatceacg cgcegatatc cactccetgg atgaactctt tcactaggct ggcctttatt gtttccggaa agqggttgct atg aaa att ggt Met Lys Ile Gly 1 ate etg ggc agt Ile Leu Gly Ser atc Ile cgc gaa ggc ege Arg Giu Gly Arg ggc caa ggc gtt Gly Gin Gly Val gee gat Ala Asp 163 211 tgg gte atg Trp Vai Met gaa etc ate Giu Leu Ile caa ate ggg get Gin Ile Gly Aia gac gca ccc gat gtg gaa ttt Asp Aia Pro Asp Val Giu Phe gac etc aaa get Asp Leu Lys Aia aac gtg ccc etg Asn Val Pro Leu gaa tee geg Glu Ser Ala aca gtt Thr Val cca ggt tee geg Pro Giy Ser Ala aaa eag tac gac Lys Gin Tyr Asp ceg cgc qta act Pro Arg Val Thr tgg tea eag gee Trp Ser Gin Ala gat gee tge gat Asp Ala Cys Asp ttCcett tte ate Phe Leu Phe Ile ace Th r ceg gaa tac aac Pro Giu Tyr Asn eac His ggt gtg ccc ggc Gly Val Pro Gly tte aaa aat geg Phe Lys Asn Ala tat gac Tyr Asp 100 ate etg gge Ile Leu Gly ggt gea gte Gly Ala Val 120 gaa tgg etg aae Giu Trp Leu Asn ace gte ggt tte Thr Val Gly Phe att tee tac Ile Ser Tyr 115 cag ate gte Gin le*Val gaa ggg ate egt Giu Gly Ile Arg gte gaa eag tgg Val Giu Gin Trp egt Arg 130 gee ace Ala Thr 135 tte aac atg tac Phe Asn Met Tyr ate ege age eag Ilie Arg Ser Gin eta Le u 145 tee ttt tee ace Ser Phe Ser Thr ace gag aae aae Thr Giu Asn Asn aac As n 155 gge aet ttt geg Gly Thr Phe Ala aat gat ega ege Asn Asp Arg Arg WO 01/00805 PCT/IBOO/00926 ggt gaa cta atc cgc ctc ctt gat agc ctc cta acg gct gtc cgc gat 643 Gly Giu Leu Ile Arg Leu Leu Asp Ser Leu Leu Thr Ala Val Arg Asp 170 175 180 taaqgctctg aaatactaat gag 666 <210> <211> 181 <212> PRT <213> Corynebacterium giutamicun <400> Met Lys Ile Gly Val Ile Leu Gly Ser 1 Gin Pro Leu Asp Phe Gly Trp Leu 145 Asn Thr Gly Asp Le u Pro Le u As n Phe Arg 130 Ser Asp Al a 5 Asp Phe Ala Thr Thr Asp Tyr Val1 Th r Pro 165 Asp Trp Giu Thr Ala Pro Ile Gly Ala Phe 150 Gly Ile 10 Gin Leu Ser Ala His 90 Giu Gi y Met Asn Arg 170 Giu Gly Ala Asp Asp Val Leu Arg Asp 140 Gly Leu Gly Al a Phe Lys Ala Pro Asn Val1 125 Ile Th r Asp Arg Tyr As n Gin Cys Gly .Lys 110 Val1 Arg Phe Ser Ph e Asp Val1 Tyr Asp Ala Thr Glu Ser Ala Leu 175 <210> 41 <211> 375 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (375) <223> RXN02809 WO 01/00805 <400> 41 PCTIBOO/00926 aac Asn 1 gca Al a at g Met cag Gin ttc Ph e ga c Asp att Ile Gly gtc Val1 aac Asn cg C Ar g cac His CC t Pro ctt Leu tct Se r 100

CCC

Pro cca Pro 5 aac As n g Ca Ala gt t Val1 aac As n gag Glu g cg Ala gac Asp gca Al a aag Lys gct Al a caa Gin ttc Ph e 70 ca g Gin aac Asn tg Trp gca Al a 9 ct Ala gag Glu aag Lys aag Lys Ctt Leu gtc Val gac Asp acc Thr 99c Gly 25 ctc Le u Gly tcc Se r gt C ValI Ca C His 105 cct Pro aac Asn 10 atc Ile C9 C Arg Ctg Leu gag Gi u t gg Trp 90 tta Leu Ctt Leu Asn 10 Ile Arg Leu Giu Trp 90 Le u gCC Ala gtc Val1 99 C Gly t g Leu cag Gin 75 acc Thr a ga Arg ttc Phe Ala Val Gly Leu Gin Th r Arg ctt Leu tcc Ser atc Ile ag c Ser 99 C Gly aag Lys acc Th r gta Val1 Leu Ser Ile S er Gly Lys Th r tcc Ser tac Tyr ctt Leu atc Ile atc Ile gca Al a CCt Pro ttc Phe 125 Ser Tyr Le u Ile Ile Al a Pro taC Tyr 9 9C Gly tcc Ser ttc Phe t cc Se r atg Met cac His 110 ctc Le u t CC Se r ga g Giu acc Thr tct Ser tcc Ser aaa Lys tcc Se r gca Ala ctt Le u gac Asp gtg Val acc Thr agt Ser <210> 42 (211> 125 <212> PRT <213> Corynebacterium glutamticum <400> 42 Asn Leu Ser Val Pro Ala Ala Leu Thr 1 5 Ala Giu Trp Asn Asn Lys Ala Ala Gly 25 Met Gly Val Arg Ala Ala Giu His Leu 40 Gin Ile Ala His Val Gin Lys Thr Gly 55 Phe Giu Tyr Pro Asn Phe Lys Pro Ser 70 Asp Ala Met Leu Giu Gin Leu Val Val Ile Arg Glu Ser Ala Asn Val Tyr His 100 105 Tyr Gly Ser Ph e Ser Met His 110 Leu Ser Giu Thr Ser Ser Lys WO 01/00805 PCTIBOO/00926 Gly Glu Leu Pro Asp Trp Asp Ser Pro Leu Phe Val Phe 115 120 125 <210> 43 <211> 273 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (273) <223> FRXA02809 <400> 43 gca gca cta acc aac gcc ctt tcc tac ctc tcc gca gag tgg aac aac 48 Ala Ala Leu Thr Asn Ala Leu Ser Tyr Leu Ser Ala Giu Trp Asn Asn 1 5 10 aag gct gca ggc atc gtc tcc tac ggc tcc gca atg ggc gtt cgc gca 96 Lys Ala Ala Gly Ile Val Ser Tyr Gly Ser Ala Met Gly Val Arg Ala 25 gct gag cac ctc cgc ggc atc ctt tcc gag ctt cag atc qca cac gtt 144 Ala Glu His Leu Arg Gly Ile Leu Ser Giu Leu Gin Ile Ala His Val 40 caa aag acc ggc ctg ctg agc atc ttc acc gac ttc gaa tac cct aac 192 Gin Lys Thr Gly Leu Leu Ser Ile Phe Thr Asp Phe Giu Tyr Pro Asn 55 ttc aag cct tcc gag cag ggc atc tcc tct gtg gac gct atg ctt gag 240 Phe Lys Pro Ser Giu Gin Gly Ile Ser Ser Val Asp Ala Met Leu Giu 70 75 cag ctt gtt gtc tgg acc aag gca atg tcc acc 273 Gin Leu Val Val Trp Thr Lys Ala Met Ser Thr <210> 44 <211> 91 <212> PRT <213> Corynebacterium giutamicum <400> 44 Ala Ala Leu Thr Asn Ala Leu Ser Tyr Leu Ser Ala Glu Trp Asn Asn 1 5 10 Lys Ala Ala Gly Ilie Val Ser Tyr Gly Ser Ala Met Gly Val Arg Ala 25 Aia Giu His Leu Arg Gly Ile Leu Ser Glu Leu Gin Ile Ala His Vai 40 Gin Lys Thr Giy Leu Leu Ser Ile Phe Thr Asp Phe Giu Tyr Pro Asn 55 Phe Lys Pro Ser Giu Gin Gly Ile Ser Ser Val Asp Ala Met Leu Giu 70 75 WO 01/00805 Gin Leu Val Val Trp Thr Lys Ala Met Ser Thr <210> <211> 5745 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (5722) <223> RXN00113 <400> tctgcgcatc ccactcgagg agctggcaaa cggccgcacc PCTJIBOO/00926 atcgatgttg aggcacgcac cgactccatc gaagagtccg catccggccg tatcgttacc gtg cgc atc gtg ctc Val Arg Ile Val Leu acc Thr cgt Arq Gi y cag Gin tct Ser gtt ValI gca Ala tcc Ser gag Glu gca Al a aa c As n gtc Val1 cct Pro att Ile ctt Leu Ct t Leu caa Gin gg t Gly 140 ct g Leu gct Ala acc Thr a tg Met tct Ser gg c Gly gag Glu ga c Asp cc t Pro 1 acc Thr cca Pro cg t Arg cca Pro aac Asn tgg Trp att Ile gt t Val1 tac T yr 145 cgc Arg gca Al a tcc Ser ttc Ph e qct Ala ctg Le u ggt Gly gac Asp 130 gag Glu ttc Ph e ga t Asp ttc Ph e gcc Ala gca Ala tcc Ser tgg Trp 115 atc Ile gt c Val1 gca Ala t cc Ser atc Ile atg Met aag Lys gct Ala 100 acc Thr a cc Thr acc Thr att le tac Tyr cgc Arg gt t Val1 ctt Leu acc Thr tac Tyr gtc Val tgc Cys 115 163 211 259 307 355 403 451 499 547 cgc att gat ggc aac. gtt gtc tcc cgc gga cag gca Arg Ilie Asp Gly Asn Val Val Ser Arg Gly Gin Ala 150 155 160 ctg ctc aag gct Leu Leu Lys Ala 165 WO 01/00805 cct tcc acc gct Pro Ser Thr Ala atg ggc caa ggc Met Gly Gin Gly 185 gag cgt gca gat Giu Arg Ala Asp 200 cag qtc att gat Gin Val Ile Asp 215 ttc gtg cac cca Phe Val His Pro 230 gcg ctc qcc gtg Ala Leu Aia Val aac gca att gtc Asn Ala Ilie Val 265 tac acc gca ctg Tyr Thr Ala Leu 280 atc gac gtt gtg Ile Asp Val Val 295 cgt gat gag aag Arg Asp Giu Lys 310 aac atg atc aac Asn Met Ilie Asn gtc gct gaa gaa Val Ala Glu Giu 345 gat ggc cag cag Asp Gly Gin Gin 360 ctc aag gct tct Leu Lys Ala Ser 375 ggc att gac gtg Giy Ile Asp Val 390 gct ttc gca gag tat Tyr 170 ga t Asp gca Ala gaa Glu gct Ala gt t Val1 250 ga C Asp gca Ala ttc Ph e ggc Gi y gtt Val1 330 tcc Ser tac Tyr g ca Al a cca Pro aag gtt Val1 cgc Arg cac His aa c As n ggg Gly 235 gcc Ala ggc Gly tcc Ser tcc Ser cgt Arg 315 gca Ala ggC Gly gca Ala tct Ser ttc Phe 395 ctg agc Se r agc S er 190 g ca Ala gag Giu aac Asn ca g Gin tac T yr 270 aac As n tcc Ser tac T yr gag Glu ctg Le u 350 ggt Gly cca Pro agc Ser ctg ggc Gly gag Giu Ctg Le u aa g Ly s acc Thr 240 gag Giu gg C Gly ttc Phe atg Met ctt Le u 320 gag Glu atc Ile ttg Leu gCt Al a ct g Le u 400 cca atc Ile gct Ala g gc Gly gtc Val 225 cag Gin cgc Arg cac His gaa Glu cac His 305 gcc Ala aac Asn gtt Val g ct Ala tac Tyr 385 cg C Arg gag ca g Gin cg c Arg ttc Ph e 210 ggg Gi y ttc Phe ctc Le u tcc Ser ctc Leu 290 tcc Ser gca Ala tgg T rp aac Asn ggc Gly 370 gtg Val cca Pro a cc gct Al a gct Al a 195 tcc Ser ga C Asp a cg Thr aag Lys ctc Le u 275 ga a Giu Ct g Leu ttc Phe gtt Val1 tac Tyr 355 ct g Le u aac Asn ggc Gly atc PCTIBOOIOO926 aag ggc 643 Lys Gly 180 gtg tgg 691 Val Trp atc cag 739 Ile Gin acc acc 787 Thr Thr cag gtc 835 Gin Val 245 gct gca 883 Ala Ala 260 ggc gag 931 Gly Giu ggc gtc 979 Gly Val gtt cca 1027 Val Pro cgt ccg 1075 Arg Pro 325 gac cgt 1123 Asp Arg 340 aac gtt 1171 Asn.Val aag gcc 1219 Lys Ala att cca 1267 Ile Pro gtt cct 1315 Val Pro 405 gac att 1363 WO 01/00805 PCTIBOO/00926 Asp Ile 420 Ala Phe Ala Glu gat Asp gag Glu gag Glu gca Ala 470 gtg Va1 atc Ile ctg Leu gag Glu gtt Va1 550 gca Ala gee Ala gee Ala gat Asp tee Ser 630 gte Val cgc Arg 425 cag Gin aag Lys ctc Leu atc Ile gte Va1 505 atg Met cag Gin gtt Va1 gca Ala gca Ala 585 atc Ile acc Thr cgt Arg ate Ile Lys 410 gg C Gly age Ser ggc Gly ctc Leu gaa Glu 490 ggc Gly gat Asp gac Asp gaa Glu get Ala 570 cct Pro atg Met gca Ala aac Asn gac Asp Leu Asp Giu Leu Leu Pro Glu Thr Ile 415 cgc Arg ttc Phe ate Ile atc Ile 475 acc Thr ttg Leu gte Va1 ace Thr gaa Glu 555 gag Glu gtt Val gtt Val gcg Ala cag Gin 635 ggc Gly tac Tyr gtg Val aag Lys 460 gag Glu cag Gin gca Ala att Ile gtt Va1 540 gaa Glu gca Ala gga Gly etc Leu gat Asp 620 atg Met gca Ala aac Asn ate Ile gac Asp tct Ser ate Ile 495 eca Pro tee Ser aae Asn gag Glu get Ala 575 ect Pro gte Va1 gag Glu gae Asp get Ala etg Leu ctt Leu ace Thr tgg Trp 480 ate Ile ace Thr ege Arg gat Asp gaa Glu 560 gca Ala gaa Glu eag Gin ace Thr atg Met 640 gae Asp gtt Val get Ala gac Asp 465 eag Gin gat Asp ctg Leu eca Pro gtt VaI 545 gca Ala get Ala ctg Leu aac Asn ctg Leu 625 tee Ser gta Val get Ala gtt Val 450 gag Glu tte Phe ace Thr ace Thr gta Val 530 ege Arg cct Pro eca Pro aag Lys aag Lys 610 ace Thr ace Thr get Ala cgt Arg 435 gtt Va1 aac Asn gca Ala gte VaI aae Asn 515 tte Phe eag Gin gca Ala gta Val tte Phe 595 ate Ile aac Asn gag Glu ace Thr 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 WO 01/00805 PCT[IBOO/00926 650 655 660 gga cgt gtt gtc acc gca gct cct ggc tac aag cct ttc gga cca gtt 2131 Gly Arg Val Val Thr Ala Ala Pro Gly Tyr Lys Pro Phe Gly Pro Val 665 670 675 ctt tcc gag acc gtt cgt gca cqt ctt cgc gca ctg acc ggt gca gca 2179 Leu Ser Glu Thr Val Arg Ala Arg Leu Arg Ala Leu Thr Gly Ala Ala 680 685 690 ggt ctg aag acc tcc tac atc ggc gat cgc gtg acc ggc acc tgg gga 2227 Gly Leu Lys Thr Ser Tyr Ile Gly Asp Arg Val Thr Gly Thr Trp Gly 695 700 705 ctt cca gaa agc tgg acc gca cac gtt gag gtt gaa ttg ctg ctg ggc 2275 Leu Pro Glu Ser Trp Thr Ala His Val Glu Val Glu Leu Leu Leu Gly 710 715 720 725 acc cgc gaa ggc gag tcc gtc cgc ggc ggc aac ctg ggt agc ctg cct 2323 Thr Arg Glu Gly Glu Ser Val Arg Gly Gly Asn Leu Gly Ser Leu Pro 730 735 740 qct aac gca tcc aqc aag ggc gac gtc gat gcg ctt atc gac gca gcc 2371 Ala Asn Ala Ser Ser Lys Gly Asp Val Asp Ala Leu Ile Asp Ala Ala 745 750 755 gtg cag aat gtt gct gca gcc aac ggc ac c agc gtc tcc atg tcc tcc 2419 Val Gln Asn Val Ala Ala Ala Asn Gly Thr Ser Val Ser Met Ser Ser 760 765 770 ggc ggt gca gct tca ggt ggc gga gtt gtt gat tcc gca gca ctt gat 2467 Gly Gly Ala Ala Ser Gly Gly Gly Val Val Asp Ser Ala Ala Leu Asp 775 780 785 gcc tac gca tcc acc gtc act ggt gaa gaa ggc gtc ctg qca aac gtt 2515 Ala Tyr Ala Ser Thr Val Thr Gly Glu Glu Gly Val Leu Ala Asn Val 790 795 800 805 gct cgc ggc atc ctg tct cag ctt ggt ctc gac acc aag gac gag gtt 2563 Ala Arg Gly Ile Leu Ser Gln Leu Gly Leu Asp Thr Lys Asp Glu Val 810 815 820 gaa ggc gca gag atc qac acc gaa ctc tac gac gct gtc gaa gca gaa 2611 Glu Gly Ala Glu Ile Asp Thr Glu Leu Tyr Asp Ala Val Glu Ala Glu 825 830 835 ctg ggc acc ggc tgg ctg aag ctt gtc acc cca gtg ttc tcc gct gat 2659 Leu Gly Thr Gly Trp Leu Lys Leu Val Thr Pro Val Phe Ser Ala Asp 840 845 850 cgt gcg atc ttg ttc gac qac cgt tgg gca tct gca cgt gaa gat ctg 2707 Arg Ala Ile Leu Phe Asp Asp Arg Trp Ala Ser Ala Arg Glu Asp Leu 855 860 865 gca cgc ctt gcc aac ggc gag gat att gcc gtc gag cgc ttt gct gga 2755 Ala Arq Leu Ala Asn Gly Glu Asp Ile Ala Val Glu Arg Phe Ala Gly 870 875 880 885 acg ggg gag acc gtc gtc aag caa gct gca tgg tgg gct gag cac gtt 2803 Thr Gly Glu Thr Val Val Lys Gln Ala Ala Trp Trp Ala Glu His Val 890 895 900 WO 01/00805 PCT/IBOO/00926 gaa gac acc gct ctc gct gca acc ctg aag cag gtt tcc gag gtg gct .2851 Glu Asp Thr Ala Leu Ala Ala Thr Leu Lys Gin Val Ser Glu Val Ala 905 910 915 gcg aag cca gcc aac gag cca cac atc gac gat gtt gcg ctg gtt acc 2899 Ala Lys Pro Ala Asn Glu Pro His Ile Asp Asp Val Ala Leu Val Thr 920 925 930 ggt gcg qct cct gag tcg atc gcc ggt gca gtt gcg gct cgc ctg ctg 2947 Gly Ala Ala Pro Glu Ser Ile Ala Gly Ala Val Ala Ala Arg Leu Leu 935 940 945 tcc cag ggc gcg acc gtc att ctc acc gca tcg aac gtc tcc cag gcg 2995 Ser Gln Gly Ala Thr Val Ile Leu Thr Ala Ser Asn Val Ser Gln Ala 950 955 960 965 cgt aaq gaa tac gca cgc aag ctc tac gct gcg aac gca acc cct aac 3043 Arg Lys Glu Tyr Ala Arg Lys Leu Tyr Ala Ala Asn Ala Thr Pro Asn 970 975 980 gca aag ctg tggj att gtt cct gcg aac atg tcc tcc tac cgc gat gtt 3091 Ala Lys Leu Trp Ile Val Pro Ala Asn Met Ser Ser Tyr Arg Asp Val 985 990 995 gat gca gtc atc gat tqg atc ggc aac gag cag cgc gtc acc gtc ggc 3139 Asp Ala Val Ile Asp Trp Ile Gly Asn Giu Gln Arg Val Thr Val Gly 1000 1005 1010 agc acc gtc acc gtg acc aag cca gct ctg acc cca acc ctt gcg tac 3187 Ser Thr Val Thr Val Thr Lys Pro Ala Leu Thr Pro Thr Leu Ala Tyr 1015 1020 1025 cca ttc gca gct cca tcc gta tcc ggt acc ttg gcg gat gca ggc cca 3235 Pro Phe Ala Ala Pro Ser Val Ser Gly Thr Leu Ala Asp Ala Gly Pro 1030 1035 1040 1045 cag gct gaa aac cag gca cgc ctg ctc ctc tgg tcc gtg gag cgc acc 3283 Gln Ala Glu Asn Gin Ala Arg Leu Leu Leu Trp Ser Val Glu Arg Thr 1050 1055 1060 atc gca gga ctt gca gat ctt gca tcc cgc ggt gtc gat gga cgc gtc 3331 Ile Ala Gly Leu Ala Asp Leu Ala Ser Arg Gly Val Asp Gly Arg Val 1065 1070 1075 cac gtt gta ctc cca ggt tcc ccg aac cgc gga atg ttc ggt ggc gac 3379 His Val Val Leu Pro Gly Ser Pro Asn Arg Gly Met Phe Gly Gly Asp 1080 1085 1090 ggc gct tac ggc gaa gtc aag gct gct ttc gac gcc atc ctt gcc aag 3427 Gly Ala Tyr Gly Giu Val Lys Ala Ala Phe Asp Ala Ile Leu Ala Lys 1095 1100 1105 tgg ggc tcc gag acc ggc tgg cca cag ttt gtc tcc ctc gca cag gca 3475 Trp Gly Ser Glu Thr Gly Trp Pro Gln Phe Val Ser Leu Ala Gln Ala 1110 1115 1120 1125 cgc atc ggc tgg gtc gca ggc acc ggc ctc atg ggt cgc aac gac gtg 3523 Arg Ile Gly Trp Val Ala Gly Thr Gly Leu Met Gly Arg Asn Asp Val 1130 1135 1140 WO 01/00805 PCT/IBOO/00926 ctc atc cct gcc gct gaa aag ctg ggc atc cac gtc tac acc cct gaa 3571 Leu Ile Pro Ala Ala Giu Lys Leu Gly Ile His Val Tyr Thr Pro Giu 1145 1150 1155 gag atc tct tcc gaa ctg ctg ggt ctt gca tcc gca gaa tcc cgc gaa 3619 Giu Ile Ser Ser Giu Leu Leu Gly Leu Ala Ser Ala Giu Ser Arg Giu 1160 1165 1170 aag gct ctg gaa gca ccg atc gat tac gac ctg acc ggt gga ctt tcc 3667 Lys Ala Leu Giu Ala Pro Ile Asp Tyr Asp Leu Thr Gly Gly Leu Ser 1175 1180 1185 ggt ggc gta tcc atc gca gca ctg gca gca tcc ctc gag tcc gac gca 3715 Gly Gly Val Ser Ile Ala Ala Leu Ala Ala Ser Leu Giu Ser Asp Ala 1190 1195 1200 1205 gta gag acc acc tct gca gca gaa gac acc atc aag gcg ctt cca tca 3763 Val Giu Thr Thr Ser Ala Ala Giu Asp Thr Ile Lys Ala Leu Pro Ser 1210 1215 1220 cct aag cac cca gag cag cca gtg ggc acg cca gtt gga gag gtc aag 3811 Pro Lys His Pro Giu Gin Pro Val Gly Thr Pro Val Gly Giu Val Lys 1225 1230 1235 acc gat ctc gaa gac atg gtt gtc atg gtt ggc gtt ggc gaa gtc tcc 3859 Thr Asp Leu Giu Asp Met Val Val Met Val Gly Val Gly Glu Val Ser 1240 1245 1250 tca tgg ggc tcc gga cgt acc cgc ttc gaa gct gag tac ggc atc cag 3907 Ser Trp Gly Ser Gly Arg Thr Arg Phe Giu Ala Giu Tyr Gly Ile Gin 1255 1260 1265 cgc gac ggc tcc gtt gac ctc acc gca gca ggc gtc ctt gag ctt gca 3955 Arg Asp Gly Ser Val Asp Leu Thr Ala Ala Gly Val Leu Giu Leu Ala 1270 1275 1280 1285 tgg atg atg ggt ctg atc tcc tgg agc gaa gat cca aag cca gcc tgg 4003 Trp Met Met Gly Leu Ile Ser Trp Ser Glu Asp Pro Lys Pro Ala Trp 1290 1295 1300 tac gac gct gac ggc acc gaa gtg cct gaa gaa gag atc tac gag cgc 4051 Tyr Asp Ala Asp Gly Thr Glu Val Pro Glu Giu Giu Ile Tyr Glu Arg 1305 1310 1315 ttc cgc gac gaa gtc atc gca cga tgc ggt gtt cgt gag ctt gtc gac 4099 Phe Arg Asp Glu Val Ile Ala Arg Cys Gly Val Arg Giu Leu Val Asp 1320 1325 1330 gac gca ttc ctc gtc gac ggc ggc tcc ctc gac gca gct gaa gtc ttc 4147 Asp Ala Phe Leu Vai Asp Gly Gly Ser Leu Asp Ala Ala Glu Val Phe 1335 1340 1345 ctc gac cgc gac atc tcc ttc tcc gta acc tct gct gaa gaa gca cag 4195 Leu Asp Arg Asp Ile Ser Phe Ser Val Thr Ser Ala Glu Giu Ala Gin 1350 1355 1360 1365 gcc tac gtc gat gca gat gct tcc gtg acc gtt gaa gaa gca gac ggc 4243 Ala Tyr Val Asp Ala Asp Ala Ser Val Thr Val Giu Glu Ala Asp Giy 1370 1375 1380 gaa tgg atc gtg acc aag aag aag ggc tcc acc tcc ttc gtg cca cgc 4291 WO 01/00805 PCT/IBOO/00926 Giu Trp Ile Val Thr Lys Lys Lys Gly Ser Thr Ser Phe Val Pro Arg 1385 1390 1395 aag gca acc ctq acc cgc tcc gta gca ggc cag ctg cca acc gac ttc 4339 Lys Ala Thr Leu Thr Arg Ser Val Ala Gly Gin Leu Pro Thr Asp Phe 1400 1405 1410 gac cct gcc aag tgg ggt atc cca gcc tcc atg atc gat gca ctc gac 4387 Asp Pro Ala Lys Trp Gly Ile Pro Ala Ser Met Ile Asp Ala Leu Asp 1415 1420 1425 aac atc gca gcg tgg aac ctg gtc act gca gtc gac qcc ttc ctg tcc 4435 Asn Ile Ala Ala Trp Asn.Leu Val Thr Ala Val Asp Ala Phe Leu Ser 1430 1435 1440 1445 tcc ggc ttc agc cca gca gaa ctc ctg cag tcc atc cac cca. gct gac 4483 Ser Gly Phe Ser Pro Ala Giu Leu Leu Gin Ser Ile His Pro Ala Asp 1450 1455 1460 gtg tcc tcc acc cag ggc acc ggt atc ggt ggc atg cag tcc cta cgc 4531 Val Ser Ser Thr Gin Gly Thr Gly Ile Gly Gly Met Gln Ser Leu Arg 1465 1470 1475 aag ctg ttc gtc aac cgc ttc ctc ggc cag gat cgt cca tcc gac atc 4579 Lys Leu Phe Val Asn Arg Phe Leu Gly Gin Asp Arg Pro Ser Asp Ile 1480 1485 1490 ctc cag gag acc ctg cca aac gtt gtg gct gca cac acc atg caq tcc 4627 Leu Gin Giu Thr Leu Pro Asn Vai Vai Ala Ala His Thr Met Gin Ser 1495 1500 1505 tac gtc ggt ggc tac ggc cag atg atc cac cca gtg gca qca tgt gca 4675 Tyr Vai Gly Gly Tyr Gly Gin Met Ilie His Pro Val Ala Ala Cys Ala 1510 1515 1520 1525 acc gca gct gtc tcc gtg gaa qaa ggc gtg gac aag atc cqc ctc aac 4723 Thr Ala Ala Vai Ser Val Glu Glu Gly Val Asp Lys Ile Arg Leu Asn 1530 1535 1540 aag gca gat ttc gtt gtc gcc ggt ggt atc gat gac atc cag gtt gaa 4771 Lys Ala Asp Phe Vai Val Ala Gly Gly Ile Asp Asp Ile Gin Val Giu 1545 1550 1555 tcc ctg acc ggc ttc ggt gac atg aac gcc acc gca gac acc cag gca 4819 Ser Leu Thr Gly Phe Gly Asp Met Asn Ala Thr Ala Asp Thr Gin Ala 1560 1565 1570 atg ctg gac aag gqc atc gac cca cgc ttc atc tcc cqc gca aac gat 4867 Met Leu Asp Lys Gly Ile Asp Pro Arg Phe Ile Ser Arg Ala Asn Asp 1575 1580 1585 cga cgt cgc gca ggc ttc ctc gaa gca gca ggt ggc ggt acc gtc ctc 4915 Arg Arg Arg Ala Gly Phe Leu Glu Ala Ala Gly*Giy Giy Thr Val Leu 1590 1595 1600 1605 ctg gca cgt gca tcc gtt gct gct gaa ctg gga ctg cca. gtt ctc gca 4963 Leu Ala Arg Ala Ser Val Ala Ala Glu Leu Gly Leu Pro Val Leu Ala 1610 1615 1620 gtt gtt gca cac gca cag tcc tac gcc gat ggt gct cac acc tcc atc 5011 Val Vai Ala His Ala Gin Ser Tyr Ala Asp Gly Ala His Thr Ser Ile WO 01/00805 PCT[IBOO/00926 1625 1630 1635 cca gca ccg gga ctt ggc gca ctg ggt gca gca cgt ggt ggc aag aag 5059 Pro Ala Pro Gly Leu Gly Ala Leu Gly Ala Ala Arg Gly Gly Lys Lys 1640 1645 1650 tcc gta ctt gct cgc gaa ctg aac aaa ttg ggt ctg acc cca gat gac 5107 Ser Val Leu Ala Arg Glu Leu Asn Lys Leu Gly Leu Thr Pro Asp Asp 1655 1660 1665 gtt cgc gtg gtc tcg aag cac gac acc tcc acc aac gcc aac gat cca 5155 Val Arg Val Val Ser Lys His Asp Thr Ser Thr Asn Ala Asn Asp Pro 1670 1675 1680 1685 aac gag tcc gag ctg cac aac ctg ctg tgg aag acc att gga cgc gaa 5203 Asn Glu Ser Glu Leu His Asn Leu Leu Trp Lys Thr Ile Gly Arg Glu 1690 1695 1700 gcc gac aac ccg atg ttc gtc gtc tcc cag aag tcc ctt acc gga cac 5251 Ala Asp Asn Pro Met Phe Val Val Ser Gln Lys Ser Leu Thr Gly His 1705 1710 1715 tca aag ggc ggt gca gca ctc ttc cag atc ggt gga ctt gtc tcc atc 5299 Ser Lys Gly Gly Ala Ala Leu Phe Gln Ile Gly Gly Leu Val Ser Ile 1720 1725 1730 ctg gaa acc ggc aag ctg cca cag aac gca tcc ctt gac tgc gtt gac 5347 Leu Glu Thr Gly Lys Leu Pro Gin Asn Ala Ser Leu Asp Cys Val Asp 1735 1740 1745 cca gag atg gaa gca aag ggc gag aac tic gtc tgg ctg cgc aag cca 5395 Pro Glu Met Glu Ala Lys Gly Glu Asn Phe Val Trp Leu Arg Lys Pro 1750 1755 1760 1765 ctg gat ctc ggc gca ggc tcc att aag gcc ggc gta ctt acc tca ctg 5443 Leu Asp Leu Gly Ala Gly Ser Ile Lys Ala Gly Val Leu Thr Ser Leu 1770 1775 1780 ggc ttc ggc cac gtg gct gca gtc gtc gig ctg gca acc agc ggc atc 5491 Gly Phe Gly His Val Ala Ala Val Val Val Leu Ala Thr Ser Gly Ile 1785 1790 1795 ttc gag cag gca atg cgc aac gca ggc ctc gac gtc gaa gca tgg cgt 5539 Phe Glu Gin Ala Met Arg Asn Ala Gly Leu Asp Val Glu Ala Trp Arg 1800 1805 1810 gca cgc gca acc cag cgc ctg cgc acc ggt gca aac cgc cta gaa gct 5587 Ala Arg Ala Thr Gin Arg Leu Arg Thr Gly Ala Asn Arg Leu Glu Ala 1815 1820 1825 ggc atg gtt ggc cga gca cca ttg ttc gag cag gtc gac gga cgt cgc 5635 Gly Met Val Gly Arg Ala Pro Leu Phe Glu Gin Val Asp Gly Arg Arg 1830 1835 1840 1845 ctg cca gag cat ggc gct cac caa gca gag atc aac ttg ctt atc gac 5683 Leu Pro Glu His Gly Ala His Gln Ala Glu Ile Asn Leu Leu Ile Asp 1850 1855 1860 gct gac gct cgc ctc ggt gct gac ggc atc tac cag ggc taaacgttag 5732 Ala Asp Ala Arg Leu Gly Ala Asp Gly Ile Tyr Gin Gly 1865 1870 WO 01/00805 atagctaaga aag <210> 46 <211> 1874 <212> PRT <213> Corynebacterium glutamicum <400> 46 Val Arg Ile Val Leu Thr Thr Glu Gly PCTIBOO/00926 5745 1 Thr Pro Arg Pro Asn Trp Ile ValI Tyr 145 Al a Ile Ala Gly Val1 225 Gin Arg Ala Ser Ph e Al a Leu GI y Asp 130 Glu Leu Gin Arg Ph e 210 Gly Phe 5 Ile Tyr Arg Val1 Leu Thr T yr Vai C ys Ala 165 Giy Trp Gln Th r Val 245 Arg Gly Gin Se r 70 Val Ala Se r Giu Arg 150 Pro Met Gi u Gin Ph e 230 Al a Gi y Ala Al a 55 Gly Giy Gin Met Arg 135 Ile S er Giy Arg Val 215 ValI Leu Ala As n Vai Pro Ile Le u Le u Gin Gly 140 Val1 Tyr Th r Thi Pro 220 Val1 Tyr Ala Glu Giu Ala His Val Gly Leu 125 Le u S er Pro Ala Arg 205 Thr Leu Ala Lys Met Al a Asp Thr His Ser 110 Asn Lys Arg Se r Se r 190 Ala Glu Asn Gin Leu Al a Thr Met Ser Giy Glu Asp Pro Gly Gin 175 Al a Asn Leu Leu Thr 255 ValI Al a Pro Th r Asp Met Val1 ValI Al a Gin 160 Gly Gi u Leu Lys Th r 240 Glu Arg Leu Lys Ala Ala Asn Ala Ile Vai Asp Gly Ser Leu Tyr Ala Gly 270 WO 01/00805 PCT/IB00/00926 His Ser Leu Gly Glu Tyr Thr Ala Leu Ala Ser Leu Gly Asn Ile Phe 275 280 285 Glu Leu Glu Gly Val Ile Asp Val Val Phe Ser Arg Gly Ser Ala Met 290 295 300 His Ser Leu Val Pro Arg Asp Glu Lys Gly Arg Ser Asn Tyr Gly Leu 305 310 315 320 Ala Ala Phe Arg Pro Asn Met Ile Asn Val Ala Ala Thr Glu Val Glu 325 330 335 Asn Trp Val Asp Arg Val Ala Glu Glu Ser Gly Glu Phe Leu Gin Ile 340 345 350 Val Asn Tyr Asn Val Asp Gly Gin Gin Tyr Ala Val Ala Gly Thr Leu 355 360 365 Ala Gly Leu Lys Ala Leu Lys Ala Ser Ala Ser Ala Asn Pro Arg Ala 370 375 380 Tyr Val Asn Ile Pro Gly Ile Asp Val Pro Phe His Ser Ser Val Leu 385 390 395 400 Arg Pro Gly Val Pro Ala Phe Ala Glu Lys Leu Asp Glu Leu Leu Pro 405 410 415 Glu Thr Ile Asp Ile Asp Ala Leu Arg Gly Arg Tyr Ile Pro Asn Leu 420 425 430 Val Ala Arg Pro Phe Glu Leu Thr Gin Ser Phe Val Asp Ala Ile Leu 435 440 445 Ala Val Val Pro Ser Glu Arg Leu Lys Gly Ile Lys Val Glu Asp Thr 450 455 460 Asp Glu Asn Thr Leu Ala Arg Leu Leu Leu Ile Glu Leu Leu Ser Trp 465 470 475 480 Gin Phe Ala Ser Pro Val Arg Trp Ile Glu Thr Gin Ala Leu Ile Ile 485 490 495 Asp Thr Val Asp Gin Ile Ile Glu Val Gly Leu Ala Ala Ser Pro Thr 500 505 510 Leu Thr Asn Leu Ala Leu Arg Thr Met Asp Val Ile Gly Lys Ser Arg 515 520 525 Pro Val Phe Asn Val Glu Arg Asp Gin Asp Thr Val Met Leu Asn Asp 530 535 540 Val Arg Gin Ala Pro Val Ala Glu Val Glu Glu Glu Ala Val Glu Glu 545 550 555 560 Ala Pro Ala Ala Ala Ala Ala Pro Ala Ala Glu Ala Pro Val Ala Ala 565 570 575 Ala Pro Val Ala Ala Ala Ala Pro Ala Pro Val Gly Asn Ala Pro Glu 580 585 590 Leu Lys Phe Asn Ala Ala Asn Ala Ile Met Val Leu Phe Ala Val Gin WO 01/00805 PCT/IB00/00926 595 600 605 Asn Lys Ile Asn Ile Asp Gin Ile Thr Ala Ala Asp Thr Ser Glu Thr 610 615 620 Leu Thr Asn Gly Val Ser Ser Arg Arg Asn Gin Met Leu Met Asp Met 625 630 635 640 Ser Thr Glu Leu Ser Val Pro Thr Ile Asp Gly Ala Ala Asp Ala Asp 645 650 655 Val Ala Thr Leu Gin Gly Arg Val Val Thr Ala Ala Pro Gly Tyr Lys 660 665 670 Pro Phe Gly Pro Val Leu Ser Glu Thr Val Arg Ala Arg Leu Arg Ala 675 680 685 Leu Thr Gly Ala Ala Gly Leu Lys Thr Ser Tyr Ile Gly Asp Arg Val 690 695 700 Thr Gly Thr Trp Gly Leu Pro Glu Ser Trp Thr Ala His Val Glu Val 705 710 715 720 Glu Leu Leu Leu Gly Thr Arg Glu Gly Glu Ser Val Arg Gly Gly Asn 725 730 735 Leu Gly Ser Leu Pro Ala Asn Ala Ser Ser Lys Gly Asp Val Asp Ala 740 745 750 Leu Ile Asp Ala Ala Val Gin Asn Val Ala Ala Ala Asn Gly Thr Ser 755 .760 765 Val Ser Met Ser Ser Gly Gly Ala Ala Ser Gly Gly Gly Val Val Asp 770 775 780 Ser Ala Ala Leu Asp Ala Tyr Ala Ser Thr Val Thr Gly Glu Glu Gly 785 790 795 800 Val Leu Ala Asn Val Ala Arg Gly Ile Leu Ser Gln Leu Gly Leu Asp 805 810 815 Thr Lys Asp Glu Val Glu Gly Ala Glu Ile Asp Thr Glu Leu Tyr Asp 820 825 830 Ala Val Glu Ala Glu Leu Gly Thr Gly Trp Leu Lys Leu Val Thr Pro 835 840 845 Val Phe Ser Ala Asp Arg Ala Ile Leu Phe Asp Asp Arg Trp Ala Ser 850 855 860 Ala Arg Glu Asp Leu Ala Arg Leu Ala Asn.Gly Glu Asp Ile Ala Val 865 870 875 880 Glu Arg Phe Ala Gly Thr Gly Glu Thr Val Val Lys Gin Ala Ala Trp 885 890 895 Trp Ala Glu His Val Glu Asp Thr Ala Leu Ala Ala Thr Leu Lys Gin 900 905 910 Val Ser Glu Val Ala Ala Lys Pro Ala Asn Glu Pro His Ile Asp Asp 915 920 925 WO 01/00805 PCT/IB00/00926 Val Ala Leu Val Thr Gly Ala Ala Pro Glu Ser Ile Ala Gly Ala Val 930 935 940 Ala Ala Arg Leu Leu Ser Gln Gly Ala Thr Val Ile Leu Thr Ala Ser 945 950 955 960 Asn Val Ser Gln Ala Arg Lys Glu Tyr Ala Arg Lys Leu Tyr Ala Ala 965 970 975 Asn Ala Thr Pro Asn Ala Lys Leu Trp Ile Val Pro Ala Asn Met Ser 980 985 990 Ser Tyr Arg Asp Val Asp Ala Val Ile Asp Trp Ile Gly Asn Glu Gin 995 1000 1005 Arg Val Thr Val Gly Ser Thr Val Thr Val Thr Lys Pro Ala Leu Thr 1010 1015 1020 Pro Thr Leu Ala Tyr Pro Phe Ala Ala Pro Ser Val Ser Gly Thr Leu 1025 1030 1035 1040 Ala Asp Ala Gly Pro Gin Ala Glu Asn Gin Ala Arg Leu Leu Leu Trp 1045 1050 1055 Ser Val Glu Arg Thr Ile Ala Gly Leu Ala Asp Leu Ala Ser Arg Gly 1060 1065 1070 Val Asp Gly Arg Val His Val Val Leu Pro Gly Ser Pro Asn Arg Gly 1075 1080 1085 Met Phe Gly Gly Asp Gly Ala Tyr Gly Glu Val Lys Ala Ala Phe Asp 1090 1095 1100 Ala Ile Leu Ala Lys Trp Gly Ser Glu Thr Gly Trp Pro Gin Phe Val 1105 1110 1115 1120 Ser Leu Ala Gin Ala Arg Ile Gly Trp Val Ala Gly Thr Gly Leu Met 1125 1130 1135 Gly Arg Asn Asp Val Leu Ile Pro Ala Ala Glu Lys Leu Gly Ile His 1140 1145 1150 Val Tyr Thr Pro Glu Glu Ile Ser Ser Glu Leu Leu Gly Leu Ala Ser 1155 1160 1165 Ala Glu Ser Arg Glu Lys Ala Leu Glu Ala Pro Ile Asp Tyr Asp Leu 1170 1175 1180 Thr Gly Gly Leu Ser Gly Gly Val Ser Ile Ala Ala Leu Ala Ala Ser 1185 1190 1195 1200 Leu Glu Ser Asp Ala Val Glu Thr Thr Ser Ala Ala Glu Asp Thr Ile 1205 1210 1215 Lys Ala Leu Pro Ser Pro Lys His Pro Glu Gin Pro Val Gly Thr Pro 1220 1225 1230 Val Gly Glu Val Lys Thr Asp Leu Glu Asp Met Val Val Met Val Gly 1235 1240 1245 WO 01/00805 PCT/IB00/00926 Val Gly Glu Val Ser Ser Trp Gly Ser Gly Arg Thr Arg Phe Glu Ala 1250 1255 1260 Glu Tyr Gly Ile Gin Arg Asp Gly Ser Val Asp Leu Thr Ala Ala Gly 1265 1270 1275 1280 Val Leu Glu Leu Ala Trp Met Met Gly Leu Ile Ser Trp Ser Glu Asp 1285 1290 1295 Pro Lys Pro Ala Trp Tyr Asp Ala Asp Gly Thr Glu Val Pro Glu Glu 1300 1305 1310 Glu Ile Tyr Glu Arg Phe Arg Asp Glu Val Ile Ala Arg Cys Gly Val 1315 1320 1325 Arg Glu Leu Val Asp Asp Ala Phe Leu Val Asp Gly Gly Ser Leu Asp 1330 1335 1340 Ala Ala Glu Val Phe Leu Asp Arg Asp Ile Ser Phe Ser Val Thr Ser 1345 1350 1355 1360 Ala Glu Glu Ala Gin Ala Tyr Val Asp Ala Asp Ala Ser Val Thr Val 1365 1370 1375 Glu Glu Ala Asp Gly Glu Trp Ile Val Thr Lys Lys Lys Gly Ser Thr 1380 1385 1390 Ser Phe Val Pro Arg Lys Ala Thr Leu Thr Arg Ser Val Ala Gly Gin 1395 1400 1405 Leu Pro Thr Asp Phe Asp Pro Ala Lys Trp Gly Ile Pro Ala Ser Met 1410 1415 1420 Ile Asp Ala Leu Asp Asn Ile Ala Ala Trp Asn Leu Val Thr Ala Val 1425 1430 1435 1440 Asp Ala Phe Leu Ser Ser Gly Phe Ser Pro Ala Glu Leu Leu Gin Ser 1445 1450 1455 Ile His Pro Ala Asp Val Ser Ser Thr Gin Gly Thr Gly Ile Gly Gly 1460 1465 1470 Met Gin Ser Leu Arg Lys Leu Phe Val Asn Arg Phe Leu Gly Gin Asp 1475 1480 1485 Arg Pro Ser Asp Ile Leu Gin Glu Thr Leu Pro Asn Val Val Ala Ala 1490 1495 1500 His Thr Met Gin Ser Tyr Val Gly Gly Tyr Gly Gin Met Ile His Pro 1505 1510 1515 1520 Val Ala Ala Cys Ala Thr Ala Ala Val Ser Val Glu Glu Gly Val Asp 1525 1530 1535 Lys Ile Arg Leu Asn Lys Ala Asp Phe Val Val Ala Gly Gly Ile Asp 1540 1545 1550 Asp Ile Gin Val Glu Ser Leu Thr Gly Phe Gly Asp Met Asn Ala Thr 1555 1560 1565 Ala Asp Thr Gin Ala Met Leu Asp Lys Gly Ile Asp Pro Arg Phe Ile WO 01/00805 PCT/IB00/00926 1570 1575 1580 Ser Arg Ala Asn Asp Arg Arg Arg Ala Gly Phe Leu Glu Ala Ala Gly 1585 1590 1595 1600 Gly Gly Thr Val Leu Leu Ala Arg Ala Ser Val Ala Ala Glu Leu Gly 1605 1610 1615 Leu Pro Val Leu Ala Val Val Ala His Ala Gin Ser Tyr Ala Asp Gl'y 1620 1625 1630 Ala His Thr Ser Ile Pro Ala Pro Gly Leu Gly Ala Leu Gly Ala Ala 1635 1640 1645 Arg Gly Gly Lys Lys Ser Val Leu Ala Arg Glu Leu Asn Lys Leu Gly 1650 1655 1660 Leu Thr Pro Asp Asp Val Arg Val Val Ser Lys His Asp Thr Ser Thr 1665 1670 1675 1680 Asn Ala Asn Asp Pro Asn Glu Ser Glu Leu His Asn Leu Leu Trp Lys 1685 1690 1695 Thr Ile Gly Arg Glu Ala Asp Asn Pro Met Phe Val Val Ser Gin Lys 1700 1705 1710 Ser Leu Thr Gly His Ser Lys Gly Gly Ala Ala Leu Phe Gin Ile Gly 1715 1720 1725 Gly Leu Val Ser Ile Leu Glu Thr Gly Lys Leu Pro Gin Asn Ala Ser 1730 1735 1740 Leu Asp Cys Val Asp Pro Glu Met Glu Ala Lys Gly Glu Asn Phe Val 1745 1750 1755 1760 Trp Leu Arg Lys Pro Leu Asp Leu Gly Ala Gly Ser Ile Lys Ala Gly 1765 1770 1775 Val Leu Thr Ser Leu Gly Phe Gly His Val Ala Ala Val Val Val Leu 1780 1785 1790 Ala Thr Ser Gly Ile Phe Glu Gin Ala Met Arg Asn Ala Gly Leu Asp 1795 1800 1805 Val Glu Ala Trp Arg Ala Arg Ala Thr Gin Arg Leu Arg Thr Gly Ala 1810 1815 1820 Asn Arg Leu Glu Ala Gly Met Val Gly Arg Ala Pro Leu Phe Glu Gin 1825 1830 1835 1840 Val Asp Gly Arg Arg Leu Pro Glu His Gly Ala His Gin Ala Glu Ile 1845 1850 1855 Asn Leu Leu Ile Asp Ala Asp Ala Arg Leu Gly Ala Asp Gly Ile Tyr 1860 1865 1870 Gin Gly <210> 47 WO 01/00805 <211> 3317 <212> DNA <213> Corynebacterium glutamicum PCT/1B00100926 <220> <221> CDS <222> (3294) <223> FRXA00113 <400> 47 gct Al a 1 tcc Ser att Ile gag Glu ggc Gly t tg Leu gcc Ala a cc Thr gct Ala g cc Ala 145 c ct Pro gcg Ala tac Tyr tca* Ser acc Thr ci g Le u atc Ile t gg Trp tc Ph e aac Asn gtc ValI ctc Leu 130 aac Asn gag Glu acc Th r g ca Al a gat Asp ggc Gly gac Asp ga C Asp cca Pro tct S er gic Val1 t gg Trp 120 ca g Gin ga t Asp gt i Val t cg Ser gcg Al a 200 tac Tyr cg c Arg gg C Gly 9gc Gly g cg Al a cgc Arg ggg Gly ga c Asp aag Lys g cg Ala ca g Gin 175 aag Lys aag Lys gca Ala ggC Gi y gca Ala acc Th r atc Ile ct Le u gag Glu acc Thr cca Pro gct Al a 160 gg C Gly gaa Glu ctg Leu 48 96 144 192 240 288 336 384 432 480 528 576 624 WO 01/00805 tgg att gtt cct Trp Ilie Val Pro 210 atc gat tgg atc Ile Asp Trp Ilie 225 acc gtg acc aag Thr Val Thr Lys gct cca tcc gta Ala Pro Ser Val 260 aac cag gca cgc Asn Gin Aia Arg 275 ctt gca gat ctt Leu Aia Asp Leu 290 ctc cca ggt tc Leu Pro G.1y Ser 305 ggc gaa gtc aag Gly Giu Val Lys gag acc ggc tgg Glu Thr Gly Trp 340 tgg gtc gca ggc Trp Val Ala Gly 355 gcc gct gaa aag Ala Aia Giu Lys 370 tcc gaa ctg ctg Ser Glu Leu Leu 385 gaa gca ccg atc Giu Aia Pro Ilie tco atc gca gca Ser Ilie Ala Ala 420 g Cg Ala gg C Giy cca Pro 245 tcc Ser ctg Leu gca Ala o og Pro got Ala 325 cca Pro a cc Th r ct g Le u ggt GI y ga t Asp 405 ctg Le u aac Asn aac Asn 230 gct Ala ggt Giy ctc Leu tcc Ser aac Asn 310 gct Ala ca g Gin ggc Gi y ggc Gly ctt Leu 390 tac T yr gca Al a atg Met 215 gag Giu ctg Leu a cc Thr ctc Leu cgc Arg 295 cgo Arg ttc Phe ttt Phe ctc Leu at C Ile 375 gca Ala gac Asp gca Ala tcc Ser ca g Gin acc Thr ttg Leu t gg Trp 280 gg t Giy gg a Giy ga C Asp gtc Val1 atg Met 360 cac His too Ser ct g Le u too Ser tcc Ser cgc Arg ca Pro g cg Ala 265 too Ser gtc Val1 atg Met gcc Ala tcc Ser 345 ggt Gly gt c Val gca Al a acc Thr ctc Leu 425 tac Tyr gtc Val acc Thr 250 gat Asp gtg Vali ga t Asp ttc Ph e ato Ile 330 ct 0 Le u cgc Arg tac Tyr ga a Glu gg t Gly 410 gag Gi u cgc Arg acc Thr 235 ctt Leu g ca Ala gag Giu gga Gly gg t Gly 315 ott Le u g ca Ala aac As n acc Th r tcc Se r 395 gga Gly tcc Ser gat Asp 220 gtc Val gog Al a Giy cgc Arg cgc Arg 300 ggc Gly g cc Ala cag Gin gao Asp cct Pro 380 cgc Arg ctt Leu gac Asp gtt ValI ggc Gly tao Tyr cca Pro acc Th r 285 gt c Vai gao Asp aag Lys gca Al a gt g Val1 365 gaa Glu ga a Giu tcc Se r goa Al a gat Asp agc Ser oCa Pro o ag Gin 270 ato Ile cac His ggc Giy tgg T rp cg 0 Arg 350 ctc Leu gag Glu aag Lys ggt Gly gta Vali 430 PCT/IBOO/00926 gca gtc 672 Ala Val aco gtc 720 Thr Val 240 ttc gca 768 Phe Ala 255 got gaa 816 Ala Glu gca gga 864 Ala Giy git gta 912 Val Vai got tao 960 Ala Tyr 320 ggc too 1008 Gly Ser 335 atc ggo 1056 Ile Gly atc cct 1104 Ile Pro atc tot 1152 Ile Ser gct ctg 1200 Ala Leu 400 ggc gta 1248 Gly Val 415 gag acc 1296 Giu Thr aoc tot gca gca gaa gac acc atc aag gcg ctt cca tca Oct aag cac Thr Ser Ala Aia Giu Asp Thr Ilie Lys Aia Leu Pro Ser Pro Lys His 1344 WO 01/00805 WO 0100805PCT/IBOO/00926 cca Pro gaa Giu 465 tcc Ser t cc Ser ggt Gly ga c Asp gaa Glu 545 ctc Leu gac Asp gat Asp gtg Val ctg Le u 625 aag L ys gcg Ala a gc Ser acc gag Glu 450 ga c Asp gga Gi y gt t Val, ct g Leu ggC Gi y 530 gtc Val1 gt c Val1 atc Ile gca Al a acc Th r 610 acc Th r tgg Trp tgg Trp cca Pro cag cag Gin atg Met cg t Arg ga c Asp atc Ile 515 acc Thr atc Ile ga c Asp tcc Ser ga t Asp 595 aag L ys cg c Arg ggt Gly aac Asn gca Ala 675 gg C cca gtg Pro Val gtt gtc Val Val acc cgc Thr Arg 485 ctc acc Leu Thr 500 tcc tgg Ser Trp gaa gtg Giu Val gca cga Ala Arg ggc ggc Gly Gly 565 ttc tcc Phe Ser 580 gct tcc Ala Ser aag aag Lys Lys tcc gta Ser Val atc cca Ilie Pro 645 ctg gtc Leu Vai 660 gaa ctc Glu Leu acc ggt gg C Gly atg Met 470 ttc Phe gjca Ala agc S er c ct Pro t gc Cys 550 tcC Ser gta ValI Val1 ggC Gly gca Ala 630 gcc Ala act Th r ct g Leu atc acg Thr 455 gt t Val ga a Glu g ca Ala ga a Glu ga a Glu 535 ggt Gi y ct C Leu acc Th r acc Thr tcc Ser 615 ggc Gly t cc Ser gca Ala cag Gin gg t cca Pro 9gC Gly gct Ala ggc Gly gat Asp 520 ga a Glu gtt Vali gac Asp tct Se r gt t Val1 600 acc Thr cag Gin atg Met gtc Val1 t CC Se r 680 ggc gt t ValI gtt Val1 gag Giu gtc Val 505 cca Pro gag Glu cg t Arg gca Al a gct Ala 585 gaa Giu tcC Ser ctg Le u atc Ilie gac Asp 665 atc Ile atg gga Gi y GI y tac Tyr 490 ctt Leu aag Lys atc Ile gag Glu gct Al a 570 gaa Glu gaa Glu ttc Phe cca Pro ga t Asp 650 gcc Ala ca C His cag gag Glu gaa Giu 475 ggc Gly gag Gi u cca Pro tac Tyr ctt Le u 555 gaa Gl u gaa Glu gca Al a gtg Val acc Thr 635 g ca Ala ttc Phe cca Pro t cc gt C Val1 460 gtc Val1 atc Ile ct t Le u gcc Ala gag Glu 540 gt C Val1 gt c ValI gca Al a gac Asp cca Pro 620 g ac Asp ctc Leu ct g Leu gct Al a ct a a ag Lys tcC Se r cag Gin gca Ala t gg Trp 525 cg C Arg gac Asp ttc Ph e cag Gln ggc Gly 605 cgc Arg ttc Phe gac Asp tcc Ser gac Asp 685 cg C acc Th r t ca Ser cgc Arg tgg Trp 510 t ac T yr ttc Phe gac Asp ctc Leu gcc Al a 590 gaa Glu aag Lys gac Asp aac As n tcC Ser 670 gtg Val1 aag ga t Asp t gg Trp gac Asp 495 a tg Met ga c Asp cg C Arg gca Ala ga C Asp 575 tac Tyr t gg Trp gca Ala cct Pro atc Ilie 655 gg C Gly tcc Ser ctg ctc Le u ggc Gly 480 ggc Gly atg Met gct Ala gac Asp ttc Ph e 560 cg c Arg gt c Val atc Ile acc Thr gcc Al a 640 gca Ala ttC Phe tcc Se r ttc 1392 1440 1488 1536 1584 1632 1680 1728 1776 1824 1872 1920 1968 2016 2064 2112 wo 01/00805 WO 0100805PCT/IBOO/00926 Thr Gin Gly Thr Gly Ile gtC Val1 705 acc Th r 9gC Giy gtc Val1 ttc Ph e ggc Gi y 785 aag Lys g ca Ala gca Ala cac His gg a Gly 865 gct Ala gtc Val1 gag Giu ccg Pro ctc Leu gtt ValI 725 a tg Met gaa Glu ggt Giy atg Met cca Pro 805 gaa Giu gct Al a tac T yr ctg Leu aac As n 885 ga C Asp ct g Le u gtc Val1 ggc Gly 710 gtg Val atc Ile Gly ggt Gly aac As n 790 cgc Arg gca Ala gaa GI u g cc Al a g gt Gly 870 aaa Lys acc Thr ctg Leu tCC Ser Gi y 695 cag Gin gCt Al a cac His gt g Val atc Ile 775 gcc Ala ttc Phe gca Al a ctg Leu gat Asp 855 gca Ala ttg Leu tcc Ser t gg Trp cag Gin cgt Arg cac His gt g Val1 745 aag Ly s gac Asp gca Ala tcc Ser gg c Gly 825 ct g Leu gct Ala cg t Arg ctg Leu aac Asn 905 acc Thr tcc Se r Gly Met Gin Ser Leu Arg Lys Leu Phe C ca Pro a cc Thr 730 gca Ala atc Ile a tc Ile gac Asp cgc Arg 810 ggt Gly cca Pro cac His ggt Gly acc Thr 890 gCC Ala att Ile ctt Leu tcc Se r 715 atg Met gca Al a cg C Arg Ca g Gin acc Th r 795 gca Al a ac Thr gtt Val1 acc Thr ggC Gly 875 cca Pro aac Asn gga Gly acc Thr 700 gac Asp ca g Gin tgt Cys ctc Leu gtt Val 780 cag Gin aac As n gt c Val1 ctc Leu tcc Se r 860 aag Lays ga t Asp gat Asp cgc Arg' gga Gi y a tc Ile t CC Se r gca Al a aac As n 765 gaa Glu gca Ala gat Asp ctc Leu gca Ala 845 atc Ile aag Lys gac Asp cca Pro gaa .GlIu 925 cac His ctc Leu tac T yr acc Thr 750 a ag Lys t CC Se r a tg Met cga Arg ctg Leu 830 gtt Val1 cca Pro tc Ser gt t Val1 aac Asn 910 gcc Al a t ca Ser cag Gin gtc Val1 735 g ca Ala g ca Al a ctg Leu ct q Le u cg t Arg 815 gca Ala gtt Val gca Ala gta Val cgc Arg 895 gag Giu gac Asp aag Lys gag Glu 720 gg t Gly gct Ala ga t Asp a cc Thr gac Asp 800 cgc Arg cgt Arg gca Al a ccg Pro ctt Leu 880 gt g Val t CC Ser aac As n ggC Gly 2160 2208 2256 2304 2352 2400 2448 2496 2544 2592 2640 2688 2736 2784 2832 WO 01/00805 WO 0100805PCTIBOOIOO926 935 ggt gca gca ctc ttc cag atc ggt gga ctt gtc tcc atc ctg gaa acc 2880 Gly Ala Ala Leu Phe Gin Ile Gly Gly Leu Val Ser Ile Leu Glu Thr 945 950 955 960 ggc aag ctg cca cag aac gca tcc ctt gac tgc gtt gac cca gag atg 2928 Gly Lys Leu Pro Gin Asn Ala Ser Leu Asp Cys Val Asp Pro Glu Met 965 970 975 gaa gca aag ggc gag aac ttc gtc tgg ctg cgc aag cca ctg gat ctc 2976 Giu Ala Lys Gly Giu Asn Phe Val Trp Leu Arg Lys Pro Leu Asp Leu 980 985 990 ggc gca ggc tcc att aag gcc ggc gta ctt acc tca ctg ggc ttc ggc 3024 Giy Ala Giy Ser Ile Lys Ala Giy Vai Leu Thr Ser Leu Gly Phe Gly 995 1000 1005 cac gtg gct gca gtc gtc gtq ctg gca acc agc ggc atc ttc gag cag 3072 His Val Aia Ala Val Val Val Leu Ala Thr Ser Gly Ile Phe Glu Gin 1010 1015 1020 gca atg cgc aac gca ggc ctc gac gtc gaa gca tgg cgt gca cgc gca 3120 Aia Met Arg Asn Ala Giy Leu Asp Val Glu Ala Trp Arg Ala Arg Ala 1025 1030 1035 1040 acc cag cgc ctg cgc acc ggt gca aac cgc cta gaa gct ggc atq gtt 3168 Thr Gin Arg Leu Arg Thr Giy Ala Asn Arg Leu Glu Ala Giy Met Val 1045 1050 1055 ggc cga gca cca ttg ttc gag cag gtc gac gga cgt cgc ctg cca gag 3216 Gly Arg Ala Pro Leu Phe Giu Gin Val Asp Gly Arg Arg Leu Pro Glu 1060 1065 1070 cat ggC gct cac caa gca gag atc aac ttg ctt. atc gac gct gac gct 3264 His Gly Ala His Gin Ala Giu Ile Asn Leu Leu Ile Asp Aia Asp Ala 1075 1080 1085 cgc ctc ggt gct gac ggc Arg Leu Gly Ala Asp Gly atc tac cag Ile Tyr Gin taaacgttag atagctaaga 1090 1095 aag <210> 48 <211> 1098 <212> PRT <213> Corynebacterium giutamicum <400> 48 Ala Ser Gly Gly Gly Val Val Asp Ser 1 5 Ser Thr Val Thr Gly Glu Giu Giy Val 25 Ile Leu Ser Gin Leu Gly Leu Asp Thr 40 Glu Ile Asp Thr Glu Leu Tyr Asp Ala 3314 3317 Ala Ala Leu Asp Ala Tyr Ala 10. Leu Ala Asn Val Ala Arg Gly Lys Asp Glu Val Giu Gly Ala Val Giu Ala Giu Leu Gly Thr Gly Leu Ala Thr Ala Ala 145 Pro Ala Tyr T rp Ile 225 Th r Al a As n Le u Leu 305 Gly Glu Trp Ala WO 01/00805 Trp Leu L Phe Asp As Asn Gly GI Val Val Ly 115 Leu Ala A] 130 Asn Glu Pr Glu Ser Il Thr Val I] 18 Ala Arg L) 195 Ilie Val Pr 210 Asp Trp Il Val Thr L) Pro Ser Va 2( Gln Ala Ai 275 Ala Asp LE 290 Pro Gly SE Glu Val L~ Thr Gly Ti Val Ala GJ 355 Ala Glu L~ 370 '0.

rs ao I0 eS eo '0 eS Leu Arg Asp Gin Thr His Ala 165 Leu Leu Al a Gly Pro 245 Ser Leu Ala Pro Al a 325 Pro Thr Le u PCTIEBOO/00926 Val 70 Trp Ile Ala Le u Ile 150 Gi y Thr Tyr Asn As n 230 Ala Gly Leu Se r Asn 310 Ala Gin Gi y Gly 55 Thr Al a Ala Ala Lys 135 Asp Al a Al a Al a Met 215 Giu Le u Thr Leu Arg 295 Arg Phe Phe L eu Ile 375 Pro Ser Val1 Trp 120 Gin Asp Val1 Ser Al a 200 Ser Gin Thr Leu Trp 280 Gi y Gly Asp Val1 Met 360 His Val1 Al a Glu 105 Trp Val1 Val1 Al a As n 185 Asn Ser Ar g Pro Ala 265 S er Val1 Met Ala Ser 345 Gly Val Phe Arg 90 Arg Ala Ser Ala Al a 170 Val1 Ala Tyr ValI Thr 250 Asp Val Asp Ph e Ile 330 Leu Arg Tyr Ser 75 Giu Ph e Glu Glu Leu 155 Arg Ser Th r Arg Th r 235 Leu Ala Glu Gly GI y 315 Leu Ala Asn Thr Ala Asp Al a His Val 140 Val Leu Gin Pro Asp 220 Val Ala Gly Arg Arg 300 Gly Al a Gln Asp Pro 380 Asp Leu Gly ValI 125 Al a Thr Leu Ala Asn 205 Val1 Gly Tyr Pro Th r 285 Val1 Asp Lys Al a Val 365 Glu Arg Ala Thr 110 Giu Ala Gi y Ser Arg 190 Al a Asp Se r Pro* Gin 270 Ile His Gi y Trp Arg 350 L eu Glu Al a Arg Gly Asp Lys Ala Gin 175 Lys Lys Ala Thr Phe 255 Ala Al a Val Ala Gly 335 Ile Ile Ile Ile Leu Glu Thr Pro Al a 160 Gly Gi u Leu Val1 Val1 240 Ala Giu Gly Val Tyr 320 Se r Gly Pro S er wo 01/00805 WO 0100805PCT/IBOO/00926 Ser 385 Glu Se r Th r Pro Glu 465 Ser Ser Gly Asp Glu 545 Leu Asp Asp Val1 Leu 625 Lys Al a Ser Thr Glu Al a Ile Se r Gi u 450 Asp Gly ValI Le u Gly 530 Val1 Val1 Ile Ala Thr 610 Th r T rp T rp Pro Gin 690 Leu Pro Al a Ala 435 Gin Met Arg Asp Ile 515 Thr Ile Asp Ser Asp 595 Lys Arg Gly Asn Ala 675 Gly Leu Ile Al a 420 Al a Pro Val1 Thr Leu 500 S er Glu Al a Gly Phe 580 Ala Lys Ser Ile Le u 660 Glu Thr Gly Asp 405 Leu Glu Val Val Arg 485 Th r Trp Val Arg Gi y 565 Ser Ser Lys Val1 Pro 645 Val Leu Gly Le u 390 Tyr Al a Asp Gly Met 470 Phe Ala Ser Pro C ys 550 Ser Val Val1 Gly Ala 630 Al a Th r Leu Ile Ala Asp Ala Thr Th r 455 ValI Glu Ala Glu Glu 535 Gly Leu Thr Thr Ser 615 Gi y Ser Ala Gin Gly 695 Se r Leu Ser Ile 440 Pro Gly Al a Gly Asp 520 Giu Val1 Asp Ser Val1 600 Thr Gin Met ValI Ser 680 Gly Al a Thr Leu 425 Lys Val1 Val Glu Val1 505 Pro Giu Arg Ala Ala 585 Giu Ser Le u Ile Asp 665 Ile Met Glu Gly 410 Glu Ala Gly Gly Tyr 490 Leu Lys Ile Gi u Ala 570 Gi u Glu Ph e Pro Asp 650 Ala His Gin Se r 395 Gi y Ser Leu Giu Gi u 475 Gi y Giu Pro Tyr Leu 555 Glu Gi u Al a Val1 Th r 635 Ala Phe Pro Ser Arg Le u Asp Pro Val 460 Val1 Ile Leu Ala Giu 540 Vai Val Ala Asp Pro 620 Asp Leu Leu Ala Leu 700 Glu Ser Al a Ser 445 Lys Ser Gin Al a Trp 525 Arq Asp Ph e Gin Gly 605 Arg Ph e Asp Ser Asp 685 Arq Lys Gi y Val 430 Pro Th r S er Arg Trp 510 T yr Phe Asp Leu Al a 590 Giu Lys Asp Asn Ser 670 Val1 Lys Ala Gly 415 Giu Lys Asp Trp Asp 495 Met Asp Arg Ala Asp 575 Tyr Trp Al a Pro Ile 655 Gly Ser Le u Leu 400 Val1 Thr His Le u Gly 480 Gl y Met Al a Asp Ph e 560 Arg Val1 Ile Thr Ala 640 Ala Phe Ser Ph e WO 01/00805 PCT/IB00/00926 Val Asn Arg 705 Thr Leu Pro Gly Tyr Gly Val Ser Val 755 Phe Val Val 770 Gly Phe Gly 785 Lys Gly Ile Ala Gly Phe Ala Ser Val 835 His Ala Gin 850 Gly Leu Gly 865 Ala Arg Glu Val Ser Lys Glu Leu His 915 Pro Met Phe 930 Gly Ala Ala 945 Gly Lys Leu Glu Ala Lys Gly Ala Gly 995 His Val Ala 1010 Phe Leu Gly Gin Asp Arg Pro Ser Asp Ile Leu Gin Glu 710 Asn Val Val 725 Gin Met Ile 740 Glu Glu Gly Ala Gly Gly Asp Met Asn 790 Asp Pro Arg 805 Leu Glu Ala 820 Ala Ala Glu Ser Tyr Ala Ala Leu Gly 870 Leu Asn Lys 885 His Asp Thr 900 Asn Leu Leu Val Val Ser Leu Phe Gin 950 Pro Gin Asn 965 Gly Glu Asn 980 Ser Ile Lys Ala Val Val Ala Ala His His Pro Val 745 Val Asp Lys 760 Ile Asp Asp 775 Ala Thr Ala Phe Ile Ser Ala Gly Gly 825 Leu Gly Leu 840 Asp Gly Ala 855 Ala Ala Arg Leu Gly Leu Ser Thr Asn 905 Trp Lys Thr 920 Gin Lys Ser 935 Ile Gly Gly Ala Ser Leu Phe Val Trp 985 Ala Gly Val 1000 Val Leu Ala 1015 Thr 730 Ala Ile Ile Asp Arg 810 Gly Pro His Gly Thr 890 Ala Ile Leu Leu Asp 970 Leu Leu Thr 715 Met Ala Arg Gin Thr 795 Ala Thr Val Thr Gly 875 Pro Asn Gly Thr Val 955 Cys Arg Thr Ser Gin Cys Leu Val 780 Gin Asn Val Leu Ser 860 Lys Asp Asp Arg Gly 940 Ser Val Lys Ser Gly Ser Tyr Ala Thr 750 Asn Lys 765 Glu Ser Ala Met Asp Arg Leu Leu 830 Ala Val 845 Ile Pro Lys Ser Asp Val Pro Asn 910 Glu Ala 925 His Ser Ile Leu Asp Pro Pro Leu 990 Leu Gly 1005 Ile Phe Val 735 Ala Ala Leu Leu Arg 815 Ala Val Ala Val Arg 895 Glu Asp Lys Glu Glu 975 Asp Phe Glu 720 Gly Ala Asp Thr Asp 800 Arg Arg Ala Pro Leu 880 Val Ser Asn Gly Thr 960 Met Leu Gly Gin 1020 Ala Trp Arg Ala Arg Ala Ala Met Arg Asn Ala Gly Leu Asp Val Glu WO 01/00805 PC 1025 1030 1035 Thr Gin Arg Leu Arg Thr Gly Ala Asn Arg Leu Giu Ala Giy Met Val 1045 1050 1055 Gly Arg Ala Pro Leu Phe Giu Gin Val Asp Gly Arg Arg Leu Pro Glu 1060 1065 1070 His Gly Ala His Gin Ala Giu Ile Asn Leu Leu Ile Asp Ala Asp Ala 1075 1080 1085 Arg Leu Gly Ala Asp Gly Ile Tyr Gin Gly 1090 10'95 <210> 49 <211> 6136 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <222> (101)..(6136) <223> RXN03111 <400> 49 cgtgaccgaa ttqagcagga acttcggggc cagccgactg attaaccgct ttggccag qccttttgcc ttcgctttcg ccggccaagg atatgactgg ttg aag acc ctt cgt T/IBOO/00926 1040 ga gcc Al a cg c Arg ctt Leu cca Pro gt c ValI gtg Val qtg ValI g cg gtt Val1 aat As n ttt Ph e ttc Phe cag Gin cag Gin ct g Leu ttg ggt Gly ctt Leu gat Asp g ca Ala acc Thr tcc Ser gat Asp gca gca Ala gca Ala cca Pro caa Gin ctg Leu agc Ser gcg Ala gcg Ly s gac Asp ctc Leu aac Asn C cg Pro ca g Gin gg C Gly CtC Le u cg c Thr atc Ile att Ile tcc Ser ggt Gly cgc Arg gta Val gtt Val1 115 atg Le u gtc Val 9gC Gly ga a Giu atc Ile ctt Leu ttg Le u 100 gCC Ala a cg WO 01/00805 WO 0100805PCTIBOO/00926 Ala ct g Leu tcc Ser 150 cct Pro tat Tyr att Ile cgc Arg gct Ala 230 gcg Ala gcc Ala gaa Gi u gg t Gly ga t Asp 310 gct Al a cgc Arg Gin atc Ile 135 cq C Arg g cg Ala gt t Va 1 gag Glu ggc GI y 215 g ct Al a tgg Trp gct Ala g cg Al a gg c Gly 295 tcc Se r gg C GI y gt t Val1 Le u 120 g cg Ala g aa Glu gag Glu t tg Leu g ca Ala 200 ggt Gi y ttc Phe gca Ala ga t Asp tat Tyr 280 atc Ile ttc Phe atg Met gag Glu Ile cag Gin ca g Gin atc Ile gtt Val 185 atg Met tcc Ser cat His acc Th r gt t Val1 265 gag Gi u gt t Val tat Tyr gca Ala ca c His 345 GI y gg C Gly ctt Leu cg c Arg 170 ggc Gly gct Ala g cg Ala cac His act Th r 250 ttg Le u gct Al a aag Lys gtt Val cc t Pro 330 gt t Val1 Ala ga c Asp ca g Gin 155 ccg Pro cgc Arg gcc Al a ttc Phe cca Pro 235 gct Al a gtt Val1 GI y ct g Le u ggt Gly 315 ga a Giu gat Asp Ala aac Asn 140 ca a Gin gt t Val1 cca Pro aag Lys agc Ser 220 gct Aia ggt Gi y aac As n gct Al a act Thr 300 gac: Asp ctt Leu gga Gly Ile 125 a tg Met gct Al a atc Ile ga c Asp gat Asp 205 ccc Pro a tg Met t tg Le u cct Pro cgc Arg 285 gcc Aila g cc Ala c ca Pro acc Thr Thr ccg Pro atc Ile gg t Gly ga c Asp 190 aag Lys cgt Arg aac Asn ga t Asp gtc Val1 270 tgg Trp aac Asn gca Al a gt g Val1 cca Pro 350 Arg a tg Met gac Asp ctg Leu 175 aac Asn aag Lys att Ile atg Met gtg Val1 255 ga t Asp ttc Phe atc Ile Gly ga t Asp 335 cg c Arg Thr ctg Leu g cg Ala 160 cgc Arg gct Al a gcc Al a act Thr gct Ala 240 ga a Gi u tgg Trp ct c Le u ctt Leu ca g Gin 320 tac Tyr ct g Leu gct Al a Ala t cg Ser 145 gcc Al a aac As n cg c Arg att Ile ccg Pro 225 gtg Val1 ctc Leu gta Val ga c Asp gag Glu 305 gcc Ala cag Gin gtt Val1 gg c Gly Arg 130 atc Ile tgc Cys tca Ser gtt Val1 gaa Giu 210 ctg Leu gag Glu a cc Th r gca Ala gtt Val 290 ggc Gly a ag Lys gag Glu acc Thr atg Met Met g cc Al a gcc Al a cgc Arg gtt Val1 195 ga t Asp aag Lys ca g Gin cg c .Arg cg c Arg 275 gga Gi y cgc Arg ata Ile ttc Phe a ag Lys 355 a cc Thr Thr g ga Gly gaa Glu ga t Asp 180 aag Lys aag Lys gtg Val1 acc Thr gag Glu 260 gtc Val1 cca Pro ggc Gly ttt Ph e g ca Ala 340 ttc Ph e cca Pro Gi y att Ile gt c Val 165 tct Ser gt c Val ct g Le u ca g Gin gt g Val1 245 atc Ile aac Asn ga t Asp gcg Ala ga t Asp 325 cca Pro act Thr a cc Thr 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 gag ctg acc ggc Glu Leu Thr Gly cgc acc cca atg atg ctg Arg Thr Pro Met Met Leu.

WO 01/00805 WO 0100805PCT/IBOO/00926 acc Thr gc t Ala 390 cac His ttc Phe ggc Gly ggc Gly ttg Leu 470 cca Pro gtt Val gga Gi y gg C Gly att Ile 550 act Thr acc Th r ga a Glu gtt Val 375 gag Glu atc Ile aac As n aag Lys atc Ile 455 gt c Val1 ggt Gly cca Pro cac His aag Lys 535 ggC Gly tcc Ser gct Ala ctt Leu gac Asp ctc Leu gca Ala tcC Ser cgc Arg 440 gtc Val aag Lys g cc Ala gaa Glu Ca c His 520 gt C Val tc a Ser tac Tyr gcg Al a ctt Leu 600 cct Pro gct Ala ca g Gln atg Met 425 ctt Le u atc Ilie gaa Glu atC Ile ctc Leu 505 t ct Ser cgc Arg cc t Pro ggc Gly atq Met 585 gtt Val1 gcc att gtt gca gcc qct gca aac ggt gga cac tgg 1267 Ala gg t G ly C tc Leu 410 ttc Phe gtt ValI acc Thr ctg Leu aag Lys 490

CCC

Pro tgg Trp g ca Ala gag Gi u ctg Leu 570 gca Ala tcc Ser Ile Gly 395 a cc Th r ttg Leu c ct Pro gcc Ala atg Met 475 cag Gin atc Ilie gaa Giu ct g Leu cg c Arg 555 cc a Pro acc Thr acc Th r Val 380 gga Gly gac *As p ga t Asp aag Lys ggc Gl y 460 cg t Arq gt t Val1 atc Ile gac Asp gat Asp 540 gct Ala gct Ala a ag Lys cag Gin Ala cag Gin atg Met cca Pro gcc Al a 445 att Ilie qat Asp aac As n att Ilie ctc Leu 525 aac As n gct Ala atg Met ga a Glu ggC Gi y 605 Ala gtt Val ctt Leu tac Tyr 430 cgc Arg cc t Pro ggt Gly tct Ser cag Gin 510 gat Asp gtg Val1 gat Asp Cct Pro g ca Al a 590 tct Ser Al a acc Thr gag Glu 415 ctg Leu gct Ala gaa Giu ttc Ph e gt g Val1 495 att Ile gag Glu gtg Val1 tac Tyr gt t Val1 575 a cc Th r ga t Asp Gly Gly His Trp gaa Glu gcc Ala 420 att Ile atc Ile gtt Val1 ttc Ph e aag Ly s 500 gca Ala acc Th r ggt Gly t gg Trp gtg Val 580 gt c Val1 gct Al a acc Th r 405 cag Gin ggt Gly gac Asp gca Ala a ag Lys 485 gaa Giu g gt Gly tac T yr ggc GI y tcc Ser 565 gg9t Gly aag L ys gg t Gly 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 WO 01/00805 WO 0100805PCT/tBOO/00926 ggc Gi y atc Ile 630 gag Gi u att Ile tcc Ser cct Pro cag Gin 710 caa Gin gct Aia gcc Aia gtg Vai ctg Le u 790 atc Ile gtc ValI gat Asp g ca Aia 615 ca c His gt t Vali gaa Giu atg Met gt t Vai 695 atg Met ttt Phe g ct Aia tgg Trp coo Pro 775 tgg Trp oct Pro gc t Aila gag Giu aag Ly s gag Giu gca Aila g cg Aila act Thr 680 ga t Asp ctg Le u gag Giu tac Tyr ttc Phe 760 gtc Val ca g Gin ggt Gi y gac Asp cac His 840 aac Asn atc Ile ggc Gi y att Ile 665 tac Tyr ggt Gi y gag Gi u cca Pro ccg Pro 745 ttg Le u att Ile tcc Ser gtc Vali ct g Leu 825 gat Asp gga atg gca tct ggc cgt tcc cag ctt Giy Met Aia Ser Giy Arg Ser Gin Leu ggc gca gac Giy Aia Asp 620 625 gao Asp ga t Asp 650 ggc Giy gag Giu cag Gin cgt Arg a gc S er 730 cat His ggc Giy gat Asp cac His gtc Vali 810 ctt Le u tc Se r aac Asn 635 gag Giu a ag Lys cag Gin t gg Trp g cc Aila 715 ct g Le u gcc Aila ctg Leu aag Lys gat Asp 795 gcc Aila gat Asp cgc Arg tcc Ser a cg Th r acc Thr tgg Trp att Ile 700 gag Giu a cg Thr gca Aila tgc Cys ga c Asp 780 gat Asp gt t Val cg c Arg tcc Ser ttt Phe gct Aila gcc Al a ct c Le u 685 gat Asp gcg Aia gt g Vali acc Thr cgc Arg 765 gt g Vali cg c Arg gct Aia ttt Ph e cgc Arg 845 gct Aia gtg Vai a ag Lys 670 aac As n g ct Ala cgt Arg gag Gi u gac Asp 750 a cg Th r cg t Arg t ac Tyr ggc Gi y gtc Vali 830 gac Asp aag Lys cag Gin 655 gtg Vali cgc Arg tcc Se r ttg Le u gat Asp 735 ctg Leu ccg Pro cgc Arg acc Thr atc Ile 815 ga c Asp atc Ile gct Aia 640 g cg Aila tac Tyr tac T yr tgg Trp atc Ile 720 gg c Gly ct c Le u ggC Gi y tgg Trp got Aila 800 acc Thr gcc Aila a tg Met gga Gi y cg c Arg ttc Phe ct c Le u gct Al a 705 gag Giu gt c Vai a cc Thr a ag Lys t gg T rp 785 gat Asp aa g Lys acc Thr ggc Giy cgc Arg cgg Arg gg t Giy gag Giu 690 gca Aia cag Gin gao Asp ccg Pro ct Pro 770 cgc Arg cag Gin gcc Aila ato Ile aaa Lys 850 ct t Leu ga t Asp gac Asp 675 ctg Leu cg t Arg ga t Asp aag Lys gct Aila 755 gt g Val1 tog Se r gtg Vali aac Asn gag Giu 835 gtg Vali ctt Leu gag Gi u 660 atc Ile tot Ser ttt Phe cat His ott L eu 740 gat Asp aac As n gao Asp got Aila ga a Giu 820 ogo Arg ott Le u g at Asp 645 ato Ile gg a Giy Giy gc Ala Gi y 725 gtt Val1 gt 0 Val1 ttt Phe too Ser att Ile 805 ct Pro ato Ile too Se r 1987 2035 2083 2131 2179 2227 2275 2323 2371 2419 2467 2515 2563 2611 2659 WO 01/00805 PTIOIO2 PCT[UBOO/00926 tca cct ggc aca Ser Pro Gly 855 agc ctt ggg Ser Leu Gly 870 cat agc cca His Ser Pro atg ctg act Met Leu Thr gag ctg aaa Glu Leu Lys 920 gtc cca ctg Val Pro Leu 935 acc cgc atc Thr Arg Ile 950 gct acc tgg Ala Thr Trp aac gtc acc Asn Val Thr gca cct gac Ala Pro Asp 1000 gta aag tcc Val Lys Ser 1015 ggc atg ctg Gly Met Leu 1030 gat gtc cca Asp Val Pro gta gtc gat Val Val Asp 9cc gac gca Ala Asp Ala 1080 atc cgc gga Th r ttc tgg gct ggc Phe Trp Ala Gly 860 cgc aac atc cca tcg gtg atc cac Arg Asn Ile Pro Ser Val Ile His 865 2707 cat gct gac aag tgg tcc cgc tcc gaa ttc gaa gca ttc His Ala Asp Lys Trp Ser Arg Ser Glu Phe Glu Ala Phe 875 880 885 acc ggc gcc aac ttg gtg tac gaa qac gcc gag cac gcg Thr Gly Ala Asn Leu Val Tyr Glu Asp Ala Glu His Ala 890 895 900 gtg cct ttg gcg ggt tcc acc gca ttc ggc acc acc gct Val Pro Leu Ala Gly Ser Thr Ala Phe Gly Thr Thr Ala 905 910 915 atc cgt ttc acc agc ccc atc gac gct ctg cca agc gct Ile Arg Phe Thr Ser Pro Ile Asp Ala Leu Pro Ser Ala 925 930 gtc acc cag gaa gac gct gaa gcc gcg atg ggt gaa ctg Val Thr Gin Glu Asp Ala Giu Ala Ala Met Gly Giu Leu 940 945 gca gct ggc ggc acc ctg gca act gtg aac aat ggc acc Ala Ala Gly Gly Thr Leu Ala Thr Val Asn Asn Gly Thr 955 960 965 gaa acc tcc gtc gat gcc ggc gtc atc gct gac tac aac Glu Thr Ser Val Asp Ala Gly Val Ile Ala Asp Tyr Asn 970 975 980 gca ggc tac ctg cca gca tcc gtt gtt cct gca cac acc Ala Gly Tyr Leu Pro Ala Ser Val Val Pro Ala His Thr 985 990 995 gtg ctg gtt ggc cgc gca tgg cca gca gtt ttc gct gcc Val Leu Val Gly Arg Ala Trp Pro Ala Val Phe Ala Ala 1005 1010 gca gtc atc cca ggc acc gat tcc gca tcc gtt gtg gaa Ala Val Ile Pro Gly Thr Asp Ser Ala Ser Val Val Giu 1020 1025 tcc ctg gtt cac ctg gag cac cac att gtg ctc aaq tcc Ser Leu Val His Leu Glu His His Ile Val Leu Lys Ser 1035 1040 acc gac ggc gcg ctg aag gtt tcc gcg act gcc gat gag Thr Asp Gly Ala Leu Lys Val Ser Ala Thr Ala Asp Glu 1050 1055 1060 acc gac ctg ggt cgc ctc gtg atc gtg cgc gca gaa atc Thr Asp Leu Gly Arg Leu Val Ile Val Arg Ala Giu Ile 1065 1070 1075 gaa ggc aac ctg att gct acg ttg gct gag cgt ttc gcg Giu Gly Asn Leu Ile Ala Thr Leu Ala Giu Arg Phe Ala 2755 2803 2851 2899 2947 2995 3043 3091 3139 3187 3235 1045 3283 3331 3379 3427 cgc aag 1085 ggc aac gct gtc gca 1090 cgc acc aac acc tcc gca WO 01/00805 PCT/IBO/00926 Ile Arg Gly Arg Lys Gly Asn Ala Val Ala Arg Thr Asn Thr Ser Ala 1095 1100 1105 ctg cca acc acc gtg gac acc cca cgc tca gct cgc gca gtg gca acc 3475 Leu Pro Thr Thr Val Asp Thr Pro Arg Ser Ala Arg Ala Val Ala Thr 1110 1115 1120 1125 gtt gtt gca cct gaa tcc atg cgc cca ttc gct gtg atc tcc ggt gac 3523 Val Val Ala Pro Giu Ser Met Arg Pro Phe Ala Val Ile Ser Gly Asp 1130 1135 1140 cgc aac cca att cac gtc tct gat gtt gcg gct tcc ctg gct ggt ctg 3571 Arg Asn Pro Ile His Val Ser Asp Val Ala Ala Ser Leu Ala Gly Leu 1145 1150 1155 cca ggt gtg atc gtg cac ggc atg tgg acc tct gcc atc ggt gaa ctg 3619 Pro Gly Val Ile Val His Gly Met Trp Thr Ser Ala Ile Gly Glu Leu 1160 1165 1170 atc gcc ggt gca gca ttc aac gat gag cag atc caa act ccc gca gcc 3667 Ile Ala Gly Ala Ala Phe Asn Asp Glu Gin Ile Gin Thr Pro Ala Ala 1175 1180 1185 aag gtc gtg gaa tac acc gca acc atg ctg gca cca gtt ctt cca ggt 3715 Lys Val Val Giu Tyr Thr Ala Thr Met Leu Ala Pro Val Leu Pro Gly 1190 1195 1200 1205 gaa gaa att gag ttc agc gtt gag cgc tcc gca gtg gac aac cgc cca 3763 Glu Giu Ile Giu Phe Ser Val Glu Arg Ser Ala Val Asp Asn Arg Pro 1.210 1215 1220 gga atg gga gag gtc cgc acc gtt acc gca acc gtc aac ggc aac tta 3811 Gly Met Gly Giu Val Arg Thr Val Thr Ala Thr Val Asn Gly Asn Leu 1225 1230 1235 gtg ctt acc gcc acc gct gtt gtg gca gct cca tct act ttc tac gca 3859 Val Leu Thr Ala Thr Ala Val Val Ala Ala Pro Ser Thr Phe Tyr Ala 1240 1245 1250.

ttc cca ggc cag ggc att cag tcc cag ggc atg ggt atg gaa gca cgc 3907 Phe Pro Gly Gin Gly Ile Gin Ser Gin Gly Met Gly Met Giu Ala Arg 1255 1260 1265 cgt aac tct cag gca gct cgc gct atc tgg gac cgc gcc gat gca cac 3955 Arg Asn Ser Gin Ala Ala Arg Ala Ile Trp Asp Arg Ala Asp Ala His 1270 1275 1280 1285 acc cgc aat aag ctg ggc ttc tcc atc gtg gaa atc gtg gaa aac aac 4003 Thr Arg Asn Lys Leu Gly Phe Ser Ile yal Giu Ile Val Giu Asn Asn 1290 1295 1300 cca cgc gaa gta acc gtg gca ggg gag aag ttc ttc cac cca gac ggc 4051 Pro Arg Giu Val Thr Val Ala Gly Giu Lys Phe Phe His Pro Asp Gly 1305 1310 1315 gtt ttq tac ctc acc cag ttc acc cag gtg ggc atg gca act ctg ggc 4099 Val Leu Tyr Leu Thr Gin Phe Thr Gin Val Gly Met Ala Thr Leu Gly 1320 1325 1330 gtt gct cag atc gct gaa atg cgt gaa gca cat gcc ttg aac cag cgt 4147 Val Ala Gin Ile Ala Giu Met Arg Giu Ala His Ala Leu Asn Gin Arg WO 01/00805 PCT/IB00/00926 1335 1340 1345 gca tac ttt get gga cac tcc gtt ggt gag tac aac gcg ctt get gca 4195 Ala Tyr Phe Ala Gly His Ser Val Gly Glu Tyr Asn Ala Leu Ala Ala 1350 1355 1360 tat gct ggt gtg ctg tcc ctg gaa tcc gtt ctg gag atc gtt tac cgt Tyr Ala Gly Val Leu Ser Leu Glu Ser Val Leu Glu Ile Val Tyr Arg 1370 1375 1380 cgt ggc ttg ace atg cac cgc ttg gtg gat cgc gat gaa aac ggt ctg Arg Gly Leu Thr Met His Arg Leu Val Asp Arg Asp Glu Asn Gly Leu 1385 1390 1395 tec aac tac gcg ctc gca get ctt cgc ccc aac aag atg ggt ctg ace Ser Asn Tyr Ala Leu Ala Ala Leu Arg Pro Asn Lys Met Gly Leu Thr 1400 1405 1410 gca gac aac gtt ttc gat tac gtt gcg tct gtt tcc gaa get tcc ggt Ala Asp Asn Val Phe Asp Tyr Val Ala Ser Val Ser Glu Ala Ser Gly 1415 1420 1425 gaa ttc ctg gag atc gtt aac tac aac ttg gct ggc ctg cag tac gca Glu Phe Leu Glu Ile Val Asn Tyr Asn Leu Ala Gly Leu Gin Tyr Ala 1430 1435 1440 gtt get gga ace cag get ggt ctt gcc gcc ctt cgt gcc gat gtt gag Val Ala Gly Thr Gin Ala Gly Leu Ala Ala Leu Arg Ala Asp Val Glu 1450 1455 1460 aac cgt gca cca ggt cag cgt gcc ttc att ttg ate cct ggc att gac Asn Arg Ala Pro Gly Gln Arg Ala Phe Ile Leu Ile Pro Gly Ile Asp 1465 1470 1475 gtg cca ttc cac tcc tec aag ctg cgc gac ggt gtg ggc gcg ttc cgt Val Pro Phe His Ser Ser Lys Leu Arg Asp Gly Val Gly Ala Phe Arg 1480 1485 1490 gag cac ctt gat tcc ctg ate cca get gag ctg gat ctg gat gtg ctg Glu His Leu Asp Ser Leu Ile Pro Ala Glu Leu Asp Leu Asp Val Leu 1495 1500 1505 gtt ggc cgc tac att cca aac ttg gtg get cgc cca ttc gaa ctc act Val Gly Arg Tyr Ile Pro Asn Leu Val Ala Arg Pro Phe Glu Leu Thr 1510 1515 1520 gaa gag ttc gtg gca tcc atg gca gaa gtg gtg gag tcc acc tat gtc Glu Glu Phe Val Ala Ser Met Ala Glu Val Val Glu Ser Thr Tyr Val 1530 1535 1540 aat gag ate ttg get gat ttc aag get get tec gcc gat aag cag aag Asn Glu Ile Leu Ala Asp Phe Lys Ala Ala Ser Ala Asp Lys Gin Lys 1545 1550 1555 ctt gee cgc acg ttg ctt att gag ctg ctt gca tgg cag ttc gca tea Leu Ala Arg Thr Leu Leu Ile Glu Leu Leu Ala Trp Gin Phe Ala Ser 1560 1565 1570 1365 4243 4291 4339 4387 4435 L445 4483 4531 4579 4627 4675 L525 4723 4771 4819 cct gtg cgc tgg Pro Val Arg Trp 1575 ate gag act cag gat ctg ttg ate aag ggc ctt caa Ile Glu Thr Gin Asp Leu Leu Ile Lys Gly Leu Gin 1580 1585 4867 WO 01/00805 PCT/IB00/00926 get gag cgt ttc gtg gag gtc ggt gtt ggc tct get cca acg ctt gcc 4915 Ala Glu Arg Phe Val Glu Val Gly Val Gly Ser Ala Pro Thr Leu Ala 1590 1595 1600 1605 aac atg atg ggc cag acc ctg cgc ctt cct cag tac gcg gac gcc acc 4963 Asn Met Met Gly Gin Thr Leu Arg Leu Pro Gin Tyr Ala Asp Ala Thr 1610 1615 1620 att gag gtg tta aac att gag cgc gat cgc cca gtt gtg ttc get acc 5011 Ile Glu Val Leu Asn Ile Glu Arg Asp Arg Pro Val Val Phe Ala Thr 1625 1630 1635 gat gag gtt gtg cgt gaa gtg gcg gtt gaa gag acc cca gca get cct 5059 Asp Glu Val Val Arg Glu Val Ala Val Glu Glu Thr Pro Ala Ala Pro 1640 1645 1650 gca gaa acc act gaa acc cca gca acc cca gca acc cca gcc cct gtt 5107 Ala Glu Thr Thr Glu Thr Pro Ala Thr Pro Ala Thr Pro Ala Pro Val 1655 1660 1665 gca get gca gcc cct gcc acc ggc ggc cct cgc cca gat gac atc age 5155 Ala Ala Ala Ala Pro Ala Thr Gly Gly Pro Arg Pro Asp Asp Ile Ser 1670 1675 1680 1685 ttc act cct tct gat gcc act gaa atg ctc ate gct atc tgg acc aag 5203 Phe Thr Pro Ser Asp Ala Thr Glu Met Leu Ile Ala Ile Trp Thr Lys 1690 1695 1700 gtt cgc cca gat cag atg ggt gcc act gat tcc ate gag acc ctg gtt 5251 Val Arg Pro Asp Gin Met Gly Ala Thr Asp Ser Ile Glu Thr Leu Val 1705 1710 1715 gag ggc gtg tcc tct cgc cgt aac cag ctc ctg ctg gat ctt ggt gtg 5299 Glu Gly Val Ser Ser Arg Arg Asn Gin Leu Leu Leu Asp Leu Gly Val 1720 1725 1730 gag ttc ggc ctc ggc gca att gac gga gca gcc gat get gag ctc ggt 5347 Glu Phe Gly Leu Gly Ala Ile Asp Gly Ala Ala Asp Ala Glu Leu Gly 1735 1740 1745 gat cta aag gtc acc gtg tcc aag atg gct aag ggc tac aag gcg ttt 5395 Asp Leu Lys Val Thr Val Ser Lys Met Ala Lys Gly Tyr Lys Ala Phe 1750 1755 1760 1765 ggc cct gtg ctc tcc gat get gca get gat gcc ctg cgt cgc ctc act 5443 Gly Pro Val Leu Ser Asp Ala Ala Ala Asp Ala Leu Arg Arg Leu Thr 1770 1775 1780 ggt cct acc ggt aag cgc ccg gga tac atc gca gag cgc gtc acc ggc 5491 Gly Pro Thr Gly Lys Arg Pro Gly Tyr Ile Ala Glu Arg Val Thr Gly 1785 1790 1795 acg tgg gaa ttg ggc cag ggc tgg gct gac cac gtg gtc get gaa gtt 5539 Thr Trp Glu Leu Gly Gin Gly Trp Ala Asp His Val Val Ala Glu Val 1800 1805 1810 gtg ate ggc gcc Val Ile Gly Ala 1815 cgc gaa ggc gca tcc Arg Glu Gly Ala Ser 1820 ctg cgc ggc ggc gac ctg gcg Leu Arg Gly Gly Asp Leu Ala 1825 5587 WO 01/00805 PCTABOO/00926 tca ctg tct cct gca agc cca gcg tct gca tca gat ctt gat tcg ctt 5635 Ser Leu Ser Pro Ala Ser Pro Ala Ser Ala Ser Asp Leu Asp Ser Leu 1830 1835 1840 1845 atc gac gca gcc gtc cag gcc gta gcc tcc cgc cgc ggc gtt gcg gtc 5683 Ile Asp Ala Ala Val Gin Ala Val Ala Ser Arg Arg Gly Val Ala Val 1850 1855 1860 tcc ctg cct tca gca ggc ggc gct gcc ggt ggc gtg gtt gat tcc gca 5731 Ser Leu Pro Ser Ala Gly Gly Ala Ala Gly Gly Val Val Asp Ser Ala 1865 1870 1875 gct ctt ggc gag ttt gca gag cag gtc acc gga cac gat ggt gtg ctt 5779 Ala Leu Gly Giu Phe Ala Giu Gin Val Thr Gly His Asp Gly Val Leu 1880 1885 1890 gct cag gca gcc cgc acc atc ttg acc cag ttg ggt ctt gat aag cca 5827 Ala Gin Ala Ala Arg Thr Ile Leu Thr Gin Leu Gly Leu Asp Lys Pro 1895 1900 1905 gca acc gtt tcc gtg gaa gac acc gca gag gaa gac ctc tac gag ttg 5875 Ala Thr Vai Ser Val Glu Asp Thr Ala Giu Giu Asp Leu Tyr Giu Leu 1910 1915 1920 1925 gtc tcc aag gaa ctc ggt tct gat tgg cca cgt cag gtt gca cca agc 5923 Val Ser Lys Glu Leu Gly Ser Asp Trp Pro Arg Gin Vai Ala Pro Ser 1930 1935 1940 ttc gat gaa gaa aag gtt gtt ctg ctt gat gac cgt tgg gct tct gcg 5971 Phe Asp Giu Giu Lys Val Val Leu Leu Asp Asp Arg Trp Ala Ser Ala 1945 1950 1955 cgt gag gat ctc tcc gcg ttg ctc ttg gcg aac tcg cag caa ctg ata 6019 Arg Giu Asp Leu Ser Ala Leu Leu Leu Ala Asn Ser Gin Gin Leu Ile 1960 1965 1970 tcg atg tca cag gcg cag gcg aag ctg ttg cag cac aag ctg aat tct 6067 Ser Met Ser Gin Ala Gin Ala Lys Leu Leu Gin His Lys Leu Asn Ser 1975 1980 1985 ttg gac ttg atg atc tcg cag cta ggc tcg cga aca aag ctt ctt gga 6115 Leu Asp Leu Met Ile Ser Gin Leu Gly Ser Arg Thr Lys Leu Leu Gly 1990 1995 2000 2005 cta cgc cga caa tgt tgc ggc 6136 Leu Arg Arg Gin Cys Cys Gly 2010 <210> <211> 2012 <212> PRT <213> Corynebacterium giutamicum <400> Leu Lys Thr Leu Arg Ala Ala Val Ala Ala Gly Ala Gly Thr Asn Val 1 5 10 Ser Asp Ilie Val Giu Arg Aia Asn Ala Leu Leu Ala Leu Val Ala Asp 25 WO 01/00805 PCT/IB00/00926 Asp Asn Val Ala Gin Glu Ala Ser 145 Ala Asn Arg Ile Pro 225 Val Leu Val Asp Glu 305 Ala Gin Val Leu Asn Pro Gin Gly Leu Arg 130 Ile Cys Ser Val Glu 210 Leu Glu Thr Ala Val 290 Gly Lys Glu Thr Ile Ser Gly Arg Val Val 115 Met Ala Ala Arg Val 195 Asp Lys Gin Arg Arg 275 Gly Arg Ile Phe Lys Gly Glu Ile Leu Leu 100 Ala Thr Gly Glu Asp 180 Lys Lys Val Thr Glu 260 Val Pro Gly Phe Ala 340 Phe Thr Asp Phe Asp Gly Ile Gly Ile Val 165 Ser Val Leu Gin Val 245 Ile Asn Asp Ala Asp 325 Pro Thr Leu Pro Val 70 Val Val Ala Leu Ser 150 Pro Tyr Ile Arg Ala 230 Ala Ala Glu Gly Asp 310 Ala Arg Glu Pro Ala 55 Ser Asp His Gin Ile 135 Arg Ala Val Glu Gly 215 Ala Trp Ala Ala Gly 295 Ser Gly Val Leu Phe 40 Phe Gin Gin Leu Leu 120 Ala Glu Glu Leu Ala 200 Gly Phe Ala Asp Tyr 280 Ile Phe Met Glu Thr Gly Phe Asp Thr Ile Ala Ala Val 90 Leu Asn 105 Ile Gly Gin Gly Gin Leu Ile Arg 170 Val Gly 185 Met Ala Ser Ala His His Thr Thr 250 Val Leu 265 Glu Ala Val Lys Tyr Val Ala Pro 330 His Val 345 Gly Arg Asp Ala Thr 75 Ser Asp Ala Asp Gin 155 Pro Arg Ala Phe Pro 235 Ala Val Gly Leu Gly 315 Glu Asp Thr Pro Gin Leu Ser Ala Ala Asn 140 Gin Val Pro Lys Ser 220 Ala Gly Asn Ala Thr 300 Asp Leu Gly Pro Val Ser Asp Ile Thr Ile 125 Met Ala Ile Asp Asp 205 Pro Met Leu Pro Arg 285 Ala Ala Pro Thr Met Ala Ala Ser Gly Arg 110 Thr Pro Ile Gly Asp 190 Lys Arg Asn Asp Val 270 Trp Asn Ala Val Pro 350 Met Trp Ala Val Ser Leu Glu His Ser Ala Asp Arg Thr Met Leu Asp Ala 160 Leu Arg 175 Asn Ala Lys Ala Ile Thr Met Ala 240 Val Glu 255 Asp Trp Phe Leu Ile Leu Gly Gin 320 Asp Tyr 335 Arg Leu Leu Ala WO 01/00805 WO 0100805PCTIIBOO/00926 Gly Asn 385 Giu Gi y Lys Gly Asp 465 Trp Al a Gly Leu Cys 545.

Thr Giy Se r T rp Gin 625 Gly Arg Phe Met 370 Gly Leu Ile Met Al a 450 Glu Ile Ile Gly Ile 530 Val1 Gly Ile Gin Val1 610 Leu Arg Arg Gly 355 Thr Gly Leu As n Gin 435 Ser Aila Ala Al a Val 515 Ala Gly Ser Leu Aila 595 Pro Gly Leu Asp Asp 675 Thr Trp Thr 405 Gin Giy Asp Aia Lys 485 Giu Gly Tyr Gi y Se r 565 Giy Lys Gly Asp Asp 645 Ile Gly Thr Aila 390 His Phe Giy Gi y Leu 470 Pro Val Giy Gly Ile 550 Thr Thr Giu Gly Ile 630 Giu Ile Ser ValI 375 Giu Ile As n Lys Ile 455 ValI Giy Pro His Lys 535 Giy Ser Aila Le u Aila 615 His Val G iu Met Asp Leu Al a Ser Arg 440 ValI Lys Aila Giu His 520 Val Ser Tyr Al a Le u 600 Lys Glu Al a Al a Th r 680 Pro Aila Gin Met 425 Leu Ile Gi u Ile Le u 505 Ser Arg Pro Gi y Met 585 Vali Asn Ile Giy Ile 665 Tyr Aila Giy Le u 410 Ph e Val1 Thr Leu Ly s 490 Pro Trp Aia Giu Le u 570 Ala Ser Giy Asp Asp 650 Giy Gi u Ile Gly 395 Thr Leu Pro Ala Met 475 Gin Ile Giu Leu Arg 555 Pro Thr Th r Met Asn 635 Gi u Lys Gin Val1 380 Gly Asp Asp Lys Gly 460 Ar q ValI Ile Asp Asp 540 Al a Ala Lys Gin Ala 620 Se r Th r Thr Trp Ala Gin Met Pro Ala 445 Ile Asp As n Ile Le u 525 Asn Ala Met Giu Giy 605 Ser Phe Ala Ala Leu 685 Al a Val1 Le u Tyr 430 Arg Pro Gly Ser Gin 510 Asp Vai Asp Pro Al a 590 Ser Gly Ala Val1 Lys 670 As n Ala Thr Giu 415 Leu Ala Giu Phe Val1 495 Ile Giu Vali T yr Val1 575 Thr Asp Arg Lys Gin 655 Val Arg Ala Pro 400 Pro Trp Asn Lys Pro 480 Leu Giu Leu Leu Val1 560 Asp Thr Giu Ser Al a 640 Ala Tyr Tyr WO 01/00805 Glu Leu Ser 690 Ala Arg Phe Gin Asp His Asp Lys Leu 740 Pro Ala Asp 755 Pro Val Asn 770 Arg Ser Asp Gin Val Ala Ala Asn Glu 820 Ile Glu Arg 835 Lys Val Leu 850 Ser Val Ile Phe Glu Ala Ala Glu His 900 Gly Thr Thr 915 Leu Pro Ser 930 Met Gly Glu Asn Asn Gly Ala Asp Tyr 980 PCT/IB00/00926 Gly Ala Gly 725 Val Val Phe Ser Ile 805 Pro Ile Ser His Phe 885 Ala Ala Ala Leu Thr 965 Asn Pro Gin 710 Gin Ala Ala Val Leu 790 Ile Val Asp Ser Ser 870 His Met Glu Val Thr 950 Ala Asn Val 695 Met Phe Ala Trp Pro 775 Trp Pro Ala Glu Pro 855 Leu Ser Leu Leu Pro 935 Arg Thr Val Asp Leu Glu Tyr Phe 760 Val Gin Gly Asp His 840 Gly Gly Pro Thr Lys 920 Leu Ile Trp Thr Gly Glu Pro Pro 745 Leu Ile Ser Val Leu 825 Asp Thr His Thr Val 905 Ile Val Ala Glu Ala 985 Ile 700 Glu Thr Ala Cys Asp 780 Asp Val Arg Ser Ala 860 Lys Asn Ala Thr Glu 940 Gly Val Leu Asp Ala Val Thr Arg 765 Val Arg Ala Phe Arg 845 Gly Trp Leu Gly Ser 925 Asp Thr Asp Pro Ala Arg Glu Asp 750 Thr Arg Tyr Gly Val 830 Asp Arg Ser Val Ser 910 Pro Ala Leu Ala Ala 990 Ser Leu Asp 735 Leu Pro Arg Thr Ile 815 Asp Ile Asn Arg Tyr 895 Thr Ile Glu Ala Gly 975 Ser Val Pro Ala His Thr Ala Pro Asp Val Leu Val Gly 995 1000 Arg Ala Trp Pro 1005 WO 01/00805 PCT/IBOO/00926 Ala Val Phe Ala Ala Val Lys Ser Ala Val Ile Pro Gly Thr Asp Ser 1010 1015 1020 Ala Ser Val Val Glu Gly Met Leu Ser Leu Val His Leu Glu His His 1025 1030 1035 1040 Ile Val Leu Lys Ser Asp Val Pro Thr Asp Gly Ala Leu Lys Val Ser 1045 1050 1055 Ala Thr Ala Asp Glu Val Val Asp Thr Asp Leu Gly Arg Leu Val Ile 1060 1065 1070 Val Arg Ala Glu Ile Ala Asp Ala Glu Gly Asn Leu Ile Ala Thr Leu 1075 1080 1085 Ala Glu Arg Phe Ala Ile Arg Gly Arg Lys Gly Asn Ala Val Ala Arg 1090 1095 1100 Thr Asn Thr Ser Ala Leu Pro Thr Thr Val Asp Thr Pro Arg Ser Ala 1105 1110 1115 1120 Arg Ala Val Ala Thr Val Val Ala Pro Glu Ser Met Arg Pro Phe Ala 1125 1130 1135 Val Ile Ser Gly Asp Arg Asn Pro Ile His Val Ser Asp Val Ala Ala 1140 1145 1150 Ser Leu Ala Gly Leu Pro Gly Val Ile Val His Gly Met Trp Thr Ser 1155 1160 1165 Ala Ile Gly Glu Leu Ile Ala Gly Ala Ala Phe Asn Asp Glu Gin Ile 1170 1175 1180 Gin Thr Pro Ala Ala Lys Val Val Glu Tyr Thr Ala Thr Met Leu Ala 1185 1190 1195 1200 Pro Val Leu Pro Gly Glu Glu Ile Glu Phe Ser Val Glu Arg Ser Ala 1205 1210 1215 Val Asp Asn Arg Pro Gly Met Gly Glu Val Arg Thr Val Thr Ala Thr 1220 1225 1230 Val Asn Gly Asn Leu Val Leu Thr Ala Thr Ala Val Val Ala Ala Pro 1235 1240 1245 Ser Thr Phe Tyr Ala Phe Pro Gly Gin Gly Ile Gin Ser Gln Gly Met 1250 1255 1260 Gly Met Glu Ala Arg Arg Asn Ser Gin Ala Ala Arg Ala Ile Trp Asp 1265 1270 1275 1280 Arg Ala Asp Ala His Thr Arg Asn Lys Leu Gly Phe Ser Ile Val Glu 1285 1290 1295 Ile Val Glu Asn Asn Pro Arg Glu Val Thr Val Ala Gly Glu Lys Phe 1300 1305 1310 Phe His Pro Asp Gly Val Leu Tyr Leu Thr Gin Phe Thr Gln Val Gly 1315 1320 1325 Met Ala Thr Leu Gly Val Ala Gin Ile Ala Glu Met Arg Glu Ala His WO 01/00805 PCT/IB00/00926 1330 1335 1340 Ala Leu Asn Gin Arg Ala Tyr Phe Ala Gly His Ser Val Gly Glu Tyr 1345 1350 1355 1360 Asn Ala Leu Ala Ala Tyr Ala Gly Val Leu Ser Leu Glu Ser Val Leu 1365 1370 1375 Glu Ile Val Tyr Arg Arg Gly Leu Thr Met His Arg Leu Val Asp Arg 1380 1385 1390 Asp Glu Asn Gly Leu Ser Asn Tyr Ala Leu Ala Ala Leu Arg Pro Asn 1395 1400 1405 Lys Met Gly Leu Thr Ala Asp Asn Val Phe Asp Tyr Val Ala Ser Val 1410 1415 1420 Ser Glu Ala Ser Gly Glu Phe Leu Glu Ile Val Asn Tyr Asn Leu Ala 1425 1430 1435 1440 Gly Leu Gin Tyr Ala Val Ala Gly Thr Gin Ala Gly Leu Ala Ala Leu 1445 1450 1455 Arg Ala Asp Val Glu Asn Arg Ala Pro Gly Gin Arg Ala Phe Ile Leu 1460 1465 1470 Ile Pro Gly Ile Asp Val Pro Phe His Ser Ser Lys Leu Arg Asp Gly 1475 1480 1485 Val Gly Ala Phe Arg Glu His Leu Asp Ser Leu Ile Pro Ala Glu Leu 1490 1495 1500 Asp Leu Asp Val Leu Val Gly Arg Tyr Ile Pro Asn Leu Val Ala Arg 1505 1510 1515 1520 Pro Phe Glu Leu Thr Glu Glu Phe Val Ala Ser Met Ala Glu Val Val 1525 1530 1535 Glu Ser Thr Tyr Val Asn Glu Ile Leu Ala Asp Phe Lys Ala Ala Ser 1540 1545 1550 Ala Asp Lys Gin Lys Leu Ala Arg Thr Leu Leu Ile Glu Leu Leu Ala 1555 1560 1565 Trp Gin Phe Ala Ser Pro Val Arg Trp Ile Glu Thr Gin Asp Leu Leu 1570 1575 1580 Ile Lys Gly Leu Gin Ala Glu Arg Phe Val Glu Val Gly Val Gly Ser 1585 1590 1595 1600 Ala Pro Thr Leu Ala Asn Met Met Gly Gin Thr Leu Arg Leu Pro Gin 1605 1610 1615 Tyr Ala Asp Ala Thr Ile Glu Val Leu Asn Ile Glu Arg Asp Arg Pro 1620 1625 1630 Val Val Phe Ala Thr Asp Glu Val Val Arg Glu Val Ala Val Glu Glu 1635 1640 1645 Thr Pro Ala Ala Pro Ala Glu Thr Thr Glu Thr Pro Ala Thr Pro Ala 1650 1655 1660 WO 01/00805 PCT/IB00/00926 Thr Pro Ala Pro Val Ala Ala Ala Ala Pro Ala Thr Gly Gly Pro Arg 1665 1670 1675 1680 Pro Asp Asp Ile Ser Phe Thr Pro Ser Asp Ala Thr Glu Met Leu Ile 1685 1690 1695 Ala Ile Trp Thr Lys Val Arg Pro Asp Gin Met Gly Ala Thr Asp Ser 1700 1705 1710 Ile Glu Thr Leu Val Glu Gly Val Ser Ser Arg Arg Asn Gin Leu Leu 1715 1720 1725 Leu Asp Leu Gly Val Glu Phe Gly Leu Gly Ala Ile Asp Gly Ala Ala 1730 1735 1740 Asp Ala Glu Leu Gly Asp Leu Lys Val Thr Val Ser Lys Met Ala Lys 1745 1750 1755 1760 Gly Tyr Lys Ala Phe Gly Pro Val Leu Ser Asp Ala Ala Ala Asp Ala 1765 1770 1775 Leu Arg Arg Leu Thr Gly Pro Thr Gly Lys Arg Pro Gly Tyr Ile Ala 1780 1785 1790 Glu Arg Val Thr Gly Thr Trp Glu Leu Gly Gin Gly Trp Ala Asp His 1795 1800 1805 Val Val Ala Glu Val Val Ile Gly Ala Arg Glu Gly Ala Ser Leu Arg 1810 1815 1820 Gly Gly Asp Leu Ala Ser Leu Ser Pro Ala Ser Pro Ala Ser Ala Ser 1825 1830 1835 1840 Asp Leu Asp Ser Leu Ile Asp Ala Ala Val Gin Ala Val Ala Ser Arg 1845 1850 1855 Arg Gly Val Ala Val Ser Leu Pro Ser Ala Gly Gly Ala Ala Gly Gly 1860 1865 1870 Val Val Asp Ser Ala Ala Leu Gly Glu Phe Ala Glu Gin Val Thr Gly 1875 1880 1885 His Asp Gly Val Leu Ala Gin Ala Ala Arg Thr Ile Leu Thr Gin Leu 1890 1895 1900 Gly Leu Asp Lys Pro Ala Thr Val Ser Val Glu Asp Thr Ala Glu Glu 1905 1910 1915 1920 Asp Leu Tyr Glu Leu Val Ser Lys Glu Leu Gly Ser Asp Trp Pro Arg 1925 1930 1935 Gin Val Ala Pro Ser Phe Asp Glu Glu Lys Val Val Leu Leu Asp Asp 1940 1945 1950 Arg Trp Ala Ser Ala Arg Glu Asp Leu Ser Ala Leu Leu Leu Ala Asn 1955 1960 1965 Ser Gin Gin Leu Ile Ser Met Ser Gin Ala Gin Ala Lys Leu Leu Gin 1970 1975 1980 WO 01/00805 PCTIBOOIOO926 His Lys LeU Asn Ser Leu Asp Leu Met Ile Ser Gin Leu Gly Ser Arg 1985 1990 1995 2000 Thr Lys Leu Leu Gly Leu Arg Arg Gin Cys Cys Gly 2005 2010 <210> 51 <211> 2185 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (2185) <223> FRXA00158 <400> 51 cgtgccattt tctttacaaa agagtattaa ccgaaacttc aggtcaggat ccacgccctg cgttcatccc ggctaagtta tacaaggagc gagttctcac gtg acc gaa ttg agc Val Thr Glu Leu Ser agg Arg ttt Phe cgt Arg gag Glu acc Th r ga t Asp ttt Phe gat Asp ggc Gly att ttc Ph e ttc Phe 9 cg Ala gca Ala cca Pro gc t Ala t cc Ser gat Asp 120 cac His cag ggg Gly gct Ala gtt Val aat Asn ttt Phe ttc Phe cag Gin 105 c agc Gin ctg Le u ttg gcc Ala ttc Phe gcc Ala gcg Ala gt Gly gat Asp atc Ilie gct Ala ctc Le u atc agc Se r gcc Ala gca Al a ct g Leu ttc Phe 75 act Th r gca Ala gtg Vai aat Asn ggt cg a Arg gg C Giy gqt Gi y ctt Leu gat Asp gca Ala acc Th r tCC Ser gat Asp 140 gca 1 ttt Phe t gg Trp gt t ValI ga t Asp gc t Ala agc Ser gag Giu tc Se r ga t Asp 145 acc ggC Gly ttg Le u agt Ser ga t Asp aac As n gtg Val1 g cg Ala cag Gin 130 gaa Gi u gca cag Gin a ag Lys gac Asp ctc Leu aac Asn ccg Pro cag Gin 115 ggc Gly ctc Le u cgc gag Giu acc Thr atc Ile att Ile tcc Se r ggt Gi y 100 cg c Arg gta Val gt t Val at g cct Pro ctt Leu gtc Val ggc Gi y ga a Giu atc Ile ctt Le u ttg Leu gcc Al a acg 163 211 259 307 355 403 451 499 547 595 WO 01/00805 Ile Ala Gin Leu 150 ggc ctg atc gcg Gly Leu Ilie Ala att tcc cgc gaa Ile Ser Arg Glu 185 gtc cct gcg gag Val Pro Ala Glu 200 tct tat gtt ttg Ser Tyr Val Leu 215 gtc att gag gca Val Ile Glu Ala 230 ctg cgc ggc ggt Leu Arg Gly Gly cag gct gct ttc Gin Ala Ala Phe 265 gtg gcg tgg gca Val Ala Trp Ala 280 atc gcc gct gat Ile Ala Ala Asp 295 aac gaa gcg tat Asn Glu Ala Tyr 310 gat ggt ggc atc Asp Gly Gly Ile gcg gat tcc ttc Ala Asp Ser Phe 345 gat gct ggc atg Asp Ala Gly Met 360 cca cgc gtt gag Pro Arg Val Glu 375 act gag ctg acc Thr Glu Leu Thr Gly 155 ggc Gly ctt Le u cgc Arg ggc Gly gct Ala 235 gcg Ala cac His act Th r ttg Leu gct Al a 315 aa g Lys gtt ValI cct Pro gtt ValI Thr ct g Le u g cg Al a cg C Arg g ct Ala 225 gcc Al a act Thr gct Al a gaa Glu t gg T rp 305 ctc Leu ct t Le u ca g Gin tac Tyr ctg Leu 385 gct Ala Al a tcg Ser g cc Ala aac As n 210 cgc Arg att Ile ccg Pro gtg Val1 ctc Leu 290 gta Val1 gac Asp gag Giu gcc Ala cag Gin 370 gtt Val ggc Gly Arg atc Ile tgc Cys 195 t ca Se r gtt ValI gaa Glu ctg Leu gag Glu 275 acc Th r gca Ala gtt ValI Gly a ag Lys 355 gag Giu acc Thr atg Met Met gcc Ala 180 gcc Ala cgc Arg gt t Val1 ga t Asp aag Lys 260 cag Gin cg C Arg cgc Arg gga Gly cg C Arg 340 ata Ile ttC Phe aag Lys acc Thr PCTIBOO/00926 Thr 165 gga 643 Gly gaa 691 Glu gat 739 Asp aag 787 Lys aag 835 Lys 245 gtg 883 Val1 acc 931 Thr gag 979 Glu gtc 1027 Val1 cca 1075 Pro 325 ggc 1123 Gly ttt 1171 Phe gca 1219 Al a ttc 1267 Phe cca 1315 Pro cgc acc cca atg atg Arg Thr Pro Met Met WO 01/00805 WO 0100805PCTIiBOO/00926 acc Thr t gg T rp acc Thr cag Gin gg t Gly 470 gac Asp gc a Ala aag Lys gaa Giu gg t Gly 550 tac T yr ggc Gly tcc Ser gg t Gly a ag Lys 630 cct Pro 410 g ct Ala cag Gin atg Met ctt Leu atc Ile 490 gaa Gi u atc Ilie ctc Le u tct Ser cg c Arg 570 cct Pro ggc Gi y atg Met gt t ValI gCC Ala gq t Gi y ctC Leu ttc Phe gtt Vai 475 acc Thr ctg Leu aag Lys

CCC

Pro t gg Trp 555 g ca Ala gag Giu ctg Le u gca Al a tcc Se r 635 att Ile 99 C Gly acc Thr ttg Leu 460 cct Pro gcc Al a atg Met cag Gin atc Ilie 540 gaa Glu ctg Leu cgc Arg cca Pro acc Thr 620 acc Thr gtt Val gga Gly ga c Asp 445 gat Asp aag Lys gg C Gly cgt Arg gt t Val 525 atc Ile gac Asp ga t Asp gct Ala gct Al a 605 aag Lys cag Gin gcc gct Ala Aia 415 gtt acc Val Thr ctt gag Leu Giu tac ctg Tyr Leu cqC gct Arg Ala 480 cct gaa Pro Giu 495 ggt ttc Giy Phe tct gtg Ser Val cag att Gin Ilie gat gag Asp Giu 560 gtg gtg Val Val 575 gat tac Asp Tyr cct gtt Pro Val gca acc Ala Thr tct gat Ser Asp 640 gca aac ggt gga cac Ala Asn Giy Gly His 420 cca Pro cca Pro t gg Trp 465 aat As n aag Lys cct Pro ttg Leu gag Giu 545 ctg Leu* ctg Leu gt C Val1 gat Asp acc Thr 625 gaa Glu gag Giu gg t Giy 450 aag Lys gg t Gly ga t Asp t gg Trp gct Al a 530 gg9t Gly ctg Le u tgt Cys ac Thr gg C Gly 610 tcc Se r tgg Trp ctg Leu 435 atc Ile atg Met g ca Ala gaa Glu atc Ile 515 atc Ile Gly atc Ile gtc Val1 ggt Gly 595 at c Ile cag Gin gtt Val1 ctg Leu aac Asn ca g Gin tcc Ser gct Ala 500 gca Al a gct Ala gt t Val1 gCC Al a ggc Gly 580 tcc Ser ttg Leu g cc Ala cct Pro ga a Glu g cc Ala att Ile atc Ile 485 gtt ValI ttc Ph e aag Lys gca Al a acc Thr 565 gg t Gly t gg Trp gt g Val gt C ValI gct Ala 645 1363 14 11 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 WO 01/00805 PCT/IB00/00926 ggt ggc gca aag aac gga atg gca tat ggc cgt tcc cag ctt ggc gca 2083 Gly Gly Ala Lys Asn Gly Met Ala Tyr Gly Arg Ser Gin Leu Gly Ala 650 655 660 gac atc cac gag atc gac aac tcc ttt gct aag gct gga cgc ctt ctt 2131 Asp Ile His Glu Ile Asp Asn Ser Phe Ala Lys Ala Gly Arg Leu Leu 665 670 675 gat gag gtt gca ggc cat gaa acg gat ttg caa gcg cgc ccg gat gag 2179 Asp Glu Val Ala Gly His Glu Thr Asp Leu Gin Ala Arg Pro Asp Glu 680 685 690 atc att 2185 Ile Ile 695 <210> 52 <211> 695 <212> PRT <213> Corynebacterium glutamicum <400> 52 Val Thr Glu Leu Ser Arg Asn Phe Gly Ala Ser Arg Leu Ile Asn Arg 1 5 10 Phe Gly Gin Glu Pro Phe Ala Phe Ala Phe Ala Gly Gin Gly Tyr Asp 25 Trp Leu Lys Thr Leu Arg Ala Ala Val Ala Ala Gly Ala Gly Thr Asn 40 Val Ser Asp Ile Val Glu Arg Ala Asn Ala Leu Leu Ala Leu Val Ala 55 Asp Asp Leu Ile Gly Thr Leu Pro Phe Gly Phe Asp Pro Val Ala Trp 70 75 Ala Asn Asn Ser Glu Asp Pro Ala Phe Asp Thr Ala Gin Ser Ala Val 90 Ser Val Pro Gly Ile Phe Val Ser Gin Ile Ala Thr Leu Asp Ser Leu 100 105 110 Glu Ala Gin Arg Leu Asp Val Asp Gin Ala Val Ser Ser Ile Gly His 115 120 125 Ser Gin Gly Val Leu Gly Val His Leu Leu Asn Asp Ala Thr Arg Ala 130 135 140 Asp Glu Leu Val Ala Ile Ala Gin Leu Ile Gly Ala Ala Ile Thr Arg 145 150 155 160 Thr Ala Arg Met Thr Gly Leu Ile Ala Gin Gly Asp Asn Met Pro Met 165 170 175 Leu Ser Ile Ala Gly Ile Ser Arg Glu Gin Leu Gin Gin Ala Ile Asp 180 185 190 Ala Ala Cys Ala Glu Val Pro Ala Glu Ile Arg Pro Val Ile Gly Leu WO 01/00805 PCT/IB00/00926 195 200 205 Arg Asn Ser Arg Asp Ser Tyr Val Leu Val Gly Arg Pro Asp Asp Asn 210 215 220 Ala Arg Val Val Lys Val Ile Glu Ala Met Ala Ala Lys Asp Lys Lys 225 230 235 240 Ala Ile Glu Asp Lys Leu Arg Gly Gly Ser Ala Phe Ser Pro Arg Ile 245 250 255 Thr Pro Leu Lys Val Gin Ala Ala Phe His His Pro Ala Met Asn Met 260 265 270 Ala Val Glu Gin Thr Val Ala Trp Ala Thr Thr Ala Gly Leu Asp Val 275 280 285 Glu Leu Thr Arg Glu Ile Ala Ala Asp Val Leu Val Asn Pro Val Asp 290 295 300 Trp Val Ala Arg Val Asn Glu Ala Tyr Glu Ala Gly Ala Arg Trp Phe 305 310 315 320 Leu Asp Val Gly Pro Asp Gly Gly Ile Val Lys Leu Thr Ala Asn Ile 325 330 335 Leu Glu Gly Arg Gly Ala Asp Ser Phe Tyr Val Gly Asp Ala Ala Gly 340 345 350 Gin Ala Lys Ile Phe Asp Ala Gly Met Ala Pro Glu Leu Pro Val Asp 355 360 365 Tyr Gin Glu Phe Ala Pro Arg Val Glu His Val Asp Gly Thr Pro Arg 370 375 380 Leu Val Thr Lys Phe Thr Glu Leu Thr Gly Arg Thr Pro Met Met Leu 385 390 395 400 Ala Gly Met Thr Pro Thr Thr Val Asp Pro Ala Ile Val Ala Ala Ala 405 410 415 Ala Asn Gly Gly His Trp Ala Glu Leu Ala Gly Gly Gly Gin Val Thr 420 425 430 Pro Glu Leu Leu Glu Thr His Ile Ala Gin Leu Thr Asp Met Leu Glu 435 440 445 Pro Gly Ile Asn Ala Gin Phe Asn Ser Met Phe Leu Asp Pro Tyr Leu 450 455 460 Trp Lys Met Gin Ile Gly Gly Lys Arg Leu Val Pro Lys Ala Arg Ala 465 470 475 480 Asn Gly Ala Ser Ile Asp Gly Ile Val Ile Thr Ala Gly Ile Pro Glu 485 490 495 Lys Asp Glu Ala Val Ala Leu Val Lys Glu Leu Met Arg Asp Gly Phe 500 505 510 Pro Trp Ile Ala Phe Lys Pro Gly Ala Ile Lys Gin Val Asn Ser Val 515 520 525 WO 01/00805 WO 0100805PCTIIBOOIOO926 Leu Ala Ile Ala Lys Glu Val Pro Glu Leu Pro Ile Ile Ile Gin Ile 530 535 540 Giu Gly Gly Val Ala Gly Gly His His Ser Trp Glu Asp Leu Asp Giu 545 550 555 560 Leu Leu Ile Ala Thr Tyr Gly Lys Val Arg Ala Leu Asp Asn Val Val 565 570 575 Leu Cys Val Gly Gly Gly Ile Gly Ser Pro Glu Arg Ala Ala Asp Tyr 580 585 590 Val Thr Gly Ser Trp Ser Thr Ser Tyr Gly Leu Pro Ala Met Pro Val 595 600 605 Asp Gly Ile Leu Val Gly Thr Ala Ala Met Ala Thr Lys Giu Ala Thr 610 615 620 Thr Ser Gin Ala Val Lys Giu Leu Leu Val Ser Thr Gin Gly Ser Asp 625 630 635 640 Glu Trp Val Pro Ala Gly Gly Ala Lys Asn Gly Met Ala Tyr Gly Arg 645 650 655 Ser Gin Leu Gly Ala Asp Ile His Glu Ile Asp Asn Ser Phe Ala Lys 660 665 670 Ala Gly Arq Leu Leu Asp Glu Val Ala Gly His Giu Thr Asp Leu Gin 675 680 685 Ala Arg Pro.-Asp Gu Ile Ile 690 695 <210> 53 <211> 3831 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(3831) <223> FRXA00572 <400> 53 cag tqq ctc aac cgc tac ctc gag ctg tct ggc cct gtt gat ggt cag 48 Gin Trp Leu Asn Arg Tyr Leu Glu Leu Ser Gly Pro Val Asp Gly Gln 1 5 10 tgg att gat gct tcc tgg gct gca cgt ttt gcc caq atg ctg gag cgt 96 Trp Ilie Asp Ala Ser Trp Ala Ala Arg Phe Ala Gin Met Led Glu Arg 25 gcc gag gcg cgt ttg atc gag cag gat cat ggc caa ttt gag cca agc 144 Ala Giu Ala Arg Leu Ile Giu Gin Asp His Gly Gin Phe Giu Pro Ser 40 ctg acg gtg gag gat ggc gtc gac aag ctt gtt gct gct tac ccg cat 192 Leu Thr Val Glu Asp Gly Val Asp Lys Leu Val Ala Ala Tyr Pro His 55 wo 01/00805 WO 0100805PCTIBOO/00926 gCC Al a ctg Le u aag Lys gat Asp gcc Ala gat Asp 145 cg C Arg tgg Trp gac Asp gcc Al a ttg Leu 225 ttc Phe cag Gln Gly tcc Se r gca Ala tgc Cys gac Asp gat Asp gtt Val1 130 cgc Ar g tcc Se r gc t Al a a ag Ly s aac As n 210 gcg Ala acc Thr gaa Glu gg C Gly gtc Val1 290 acc Th r cg c Arg gtg ValI cgc Arg 115 9 ct Ala ttt Phe cg c Arg 9gc Gly tgg Trp 195 t tg Le u ggt Gly agc Ser ga C Asp acc Thr 275 gat Asp gac Ctg ctc Asp Leu Leu 70 acg ccg ggc Thr Pro Gly cgt cgc tgg Arg Arg Trp 100 tac acc gct Tyr Thr Ala ggc atc acc Gly Ile Thr gtc gac gCC Vai Asp Ala 150 gac atc atg Asp Ile Met 165 cgc aac atc Arg Asn Ile 180 tcc cgc tcc Ser Arg Ser gtg tac gaa Val Tyr Glu tcc acc qca Ser Thr Ala 230 ccc atc gac Pro Ile Asp 245 gct gaa gcc Ala Glu Ala 260 Ctg gca act Leu Ala Thr gcc ggc gtc Ala Gly Val acc ccg gct Thr Pro Ala gat qtc gcc tgg ttc ttq Asp Val Ala Trp Phe Leu 75 a ag L ys t gg T rp ga t Asp aag Lys 135 acc Th r gqc Gly cca Pro gaa Glu gac Asp 215 ttc Phe gCt Al a gcg Ala gtg Val1 atc I le 295 cct Pro cg C Arg cag Gin 120 gcc Ala atc Ile a aa Lys t cg Se r ttc Phe 200 gCC Ala g gc Gly ctg Le u atg Met aac As n 280 gC t Ala att Ile tcc Ser gtc Val ct g Leu gat Asp aca Thr 175 cat His acc Thr gt g Val atc Ile qt c Val1 255 gca Ala ga a Glu gca Al a ggC Gi y ga t Asp cac His gtc Val1 ctt Leu tcc Ser 160 ttc Phe gct Al a ggc Gly cc t Pro cg t Arg 240 acc Thr gct Al a acc Thr ggC Gly tac Tyr 305 gtt Val atc Ile gt t ValI Gly ctq Le u 385 aac As n gg c Gi y gac Asp tcc Ser gt c Val1 465 cac H is ttc Phe acc Thr agc Ser cgc WO 01/00805 ctg cca gc Leu Pro Al ggc cgc gc Gly Arg Al cca ggc ac Pro Gly Th 34 cac ctg ga His Leu GI 355 gcg ctg aa Ala Leu Ly 370 ggt cgc ct Gly Arg Le ctg att gc Leu Ile Al aac qct gt Asn Ala Va 42 acc cca cg Thr Pro Ar 435 atg cgc cc Met Arg Pr 450 tct gat gt Ser Asp Va gqc atg tg Gly Met Tr aac gat ga Asn Asp GI gca acc at Ala Thr Me 515 gtt gag cg Val Glu Ar 530 acc gtt ac a 0

U

g t a

C

0

C

a 0' t p g

U

0 g t

C

g

C

PCTI1BOO/00926 tcc gtt gtt cct gca cac acc gca cct gac gtg ctg 960 Ser Val Val Pro Ala His Thr Ala Pro Asp Val Leu 310 315 320 t gg Trp 325 ga t Asp cac His gtt Val1 gtg Val a cg Thr 405 gca Ala tca Ser ttc Phe gcg Ala a cc Th r 485 ca g Gin ct g Le u tcc Ser gca cca Pro tcc Ser cac His t cc Se r atc Ile 390 ttg Le u cg C Arq gct Al a gct Al a gct Aila 470 tct Ser atc Ile g ca Ala g ca Ala a cc gca Al a g ca Al a att.

Ile g cg Aila 375 gtq Val gct Ala acc Thr cg C Arg gtg ValI 455 tcc Ser g cc Al a caa Gin cca Pro gt g ValI 535 gt C gtt Va 1 t cc Ser gtg Val 360 act Thr cgc Arg gag Glu aac Asn gca Al a 440 atc Ile ctg Leu atc Ile act Thr gtt ValI 520 gac Asp aac ttc Phe gtt Val 345 ctc Leu gcc Aila q ca Al a cgt Arg acc Th r 425 gtg Val1 tcc Ser gct Ala ggt.

Gi y ccc Pro 505 ctt Le u aac Asn 99C gct Ala 330 gtg Val1 aag Lys ga t Asp gaa Giu ttc Phe 410 tcc Se r gca Ala gg t Gly ggt Gly gaa Glu 490 g ca Al a cca Pro cgc Arg aac gcc Ala gaa Glu tcc Ser gag Glu atc Ile 395 gcg Al a gca Al a acc Th r gac Asp ctg Le u 475 ctg Leu g cc Al a ggt Gly cca Pro tta gta Val1 Gly gat Asp gta Val 380 g cc Ala atc Ile ctg Leu gtt Val1 cgc Arg 460 cca Pro atc Ile a ag Lys gaa Glu gga Gi y 540 gtg aag Lys atq Met gtc Val1 365 gtc Val ga c Asp cg C Arg cca Pro gtt Val1 445 aac Asn gg t Gly gcc Ala gt c Val1 ga a Glu 525 at g Met ct t gtc Val1 ctg Le u gac Asp ga C Asp Gi y 400 aag Lys gtg Val1 ga a Glu cac His gt g Val1 480 gca Ala tac Tyr ttc Phe gt c Val acc 1008 1056 1104 1152 1200 1248 1296 1344 1392 1440 1488 1536 1584 1632 1680 Arg 545 9 ct Al a att Ile gct Ala ggC Gly gtg Val 625 cag Gin ga a Giu cac His tcc Se r cac His 705 gca Al a ga t Asp gt t Val gct Al a cag Gin WO 01/00805 Thr Vai Thr gtt gtg gca Vai Val Ala cag tcc cag Gin Ser Gin 580 cgc gct atc Arg Ala Ile 595 ttc tcc atc Phe Ser Ile 610 gca ggg gag Ala Gly Giu ttc acc cag Phe Thr Gin atg cgt gaa Met Arg Giu 660 tcc gtt ggt Ser Val Gly 675 ctg gaa tcc Leu Glu Ser 690 cgc ttg gtg Arg Leu Val gct ctt cgc Ala Leu Arg tac gtt gcg Tyr Val Ala 740 aac tac aac Asn Tyr Asn 755 gqt ctt gcc Gly Leu Ala 770 cgt gcC ttc Arg Ala Phe Th r 550 cca Pro atg Met gac Asp ga a Gi u ttC Ph e 630 gg C Gly cat His tac Tyr ctq Le u cg c Arg 710 aac As n gtt Val1 gct Al a ctt Leu ttg Le u Val t ct Se r gg t Gly cgc Arg atc Ile 615 ttC Phe atg Met gcc Al a aac Asn gag Giu 695 gat Asp aag Lys tcc Ser qgC Gly cgt Arq 775 atc Ile Le u 555 gca Ala cg c Arg cac His aac As n ggc Gly 635 ggc Gly cgt Arg gca Al a cg t Arg ct g Leu 715 acc Thr ggt Gi y gc a Al a gag Giu gac Asp Val ttc Phe cgt Arg acc Thr cca Pro 620 gtt Val1 gtt Val1 qca Ala tat Tyr cgt Arq 700 t cc Ser gca Al a gaa Giu gt t Val1 aac Asn 780 gtg Val Leu cca Pro aac Asn cgc Arg 605 cgc Arg t tg Leu gct Al a tac T yr gct Ala 685 g gc Gly aac Asn gac Asp ttC Phe gct Al a 765 cg t Arg cca Pro Thr ggC Gly t ct Se r 590 aat As n gaa Gi u tac Tyr cag Gin ttt Phe 670 ggt Gly ttg Leu tac T yr aac Asn ctg Le u 750 gga Gly g ca Ala ttc Phe Ala cag Gin 575 cag Gin a ag Lys gta ValI ctc Le u atc Ile 655 gct Al a gtg Val1 acc Thr g cg Ala gt t Val1 735 gag Giu acc Th r cca Pro cac His PCTIBOO/00926 Thr 560 qgc 1728 Gly gca 1776 Ala ctg 1824 Leu acc 1872 Thr acc 1920 Thr 640 gct 1968 Al a gga 2016 Gly ctg 2064 Leu atg 2112 Met ctc 2160 Le u 720 ttc 2208 Phe atc 2256 Ile cag 2304 Gin ggt 2352 Gly tcc 2400 Ser WO 01/00805 t cC Ser ctg Le u cca Pro tcc Se r ga t Asp 865 ctt Le u gag Giu gag Giu acc Thr att Ilie 945 ga a Glu acc Th r gcc Al a gcc Al a atg Met 102~ aag ctg Lys Leu atc Cca Ile Pro aac ttg Asn Leu 835 atg gca Met Ala 850 ttc aag Phe Lys att gag Ile Glu act cag Thr Gin gtc ggt Val Gly 915 ctg cgc Leu Arg 930 gag cgc Glu Arg gtg gcg Vai Ala cca gca Pro Ala acc ggc Thr Giy 995 act gaa Thr Glu 1010 cgc Arg gct Ala 820 gtg Val1 gaa Glu gct Al a ctg Le u gat Asp 900 gt t Val1 ctt Le u ga t Asp gt t Val acc Th r 980 gg C Gly atg Met gac Asp 805 gag Glu gct Al a gtg Val gct Ala ctt Le u 885 ctg Le u ggc Gly cct Pro cgc Arg gaa Glu 965 cca Pro cct Pro ctc Le u gg t Gly ctg Le u cgc Arg gt g Val1 tcc Ser 870 gca Ala ttg Leu tct Ser ca g Gin cca Pro 950 gag Glu gca Ala cgc Arg atc Ile gtg ggc gcg Val Gly Ala gat ctg gat Asp Leu Asp 825 cca ttc gaa Pro Phe Glu 840 gag tcc acc Giu Ser Thr 855 gcc gat aag Ala Asp Lys tgg cag ttc Trp Gin Phe atc aagggc Ile Lys Gly 905 gct cca acg Ala Pro Thr 920 tac qcg gac Tyr Ala Asp 935 gtt gtg ttc Val Val Phe acc cca gca Thr Pro Ala acc cca gcc Thr Pro Ala 985 cca gat gac Pro Asp Asp 1000 gct atc tgg Ala Ile Trp 1015 cgt gag cac ctt Arg Glu His Leu ctg gtt ggc cqc Leu Val Gly Arg 830 act gaa gag ttc Thr Giu Glu Phe 845 gtc aat gag atc Val Asn Giu Ile 860 aag ctt gcc cgc Lys Leu Ala Arg 875 tca cct gtg cgc Ser Pro Val Arg caa gct gag cgt Gin Ala Glu Arg 910 gcc aac atg atg Ala Asn Met Met 925 acc att gag gtg Thr Ilie Glu Val 940 acc gat gag gtt Thr Asp Glu Val 955 cct gca gaa acc Pro Ala Giu Thr gtt gca gct gca Val Ala Ala Ala 990 agc ttc act cct Ser Phe Thr Pro 1005 aag gtt cgc cca Lys Val Arg Pro 1020 gtt gag ggc gtg Val Glu Gly Val 1035 gat Asp 815 tac Tyr gtg Val1 t tg Le u a cg Th r tgg Trp 895 ttc Phe ggc Gly tta Leu gtg Val1 act Thr 975 gcc Ala tct Ser ga t Asp t cc Se r PCTJIBOO/00926 800 tcc 2448 Ser att 2496 Ile gca 2544 Al a gct 2592 Al a ttg 2640 Leu 880 atc 2688 Ile gtg 2736 ValI caq 2784 Gln aac 2832 Asn cgt 2880 Arg 960 gaa 2928 Glu cct 2976 Pro gat 3024 Asp cag 3072 Gin tct 3120 Ser 1040 ggt qcc act gat tcc atc gag acc Gly Ala Thr Asp Ser Ile Glu Thr 1030 WO 01/00805 WO 0100805PCT/IBOO/00926 .cgc cgt aac cag ctc ctg ctg gat ctt ggt gtg gag ttc ggc ctc ggc 3168 Arg Arg Asn Gin Leu Leu Leu Asp Leu Gly Val Glu Phe Gly Leu Gly 1045 1050 1055 gca att gac gga gca gcc gat gct gag ctc ggt gat cta aag gtc acc 3216 Ala Ile Asp Gly Ala Ala Asp Ala Glu Leu Gly Asp Leu Lys Val Thr 1060 1065 1070 gtg tcc aag atg gct aag ggc tac aag gcg ttt ggc cct gtg ctc tcC 3264 Val Ser Lys Met Ala Lys Gly Tyr Lys Ala Phe Gly Pro Val Leu Ser 1075 1080 1085 gat gct gca gct gat gcc ctg cgt cgc ctc act ggt cct acc ggt aag 3312 Asp Ala Ala Ala Asp Ala Leu Arg Arg Leu Thr Gly Pro Thr Gly Lys 1090 1095 1100 cgc ccg gga tac atc gca gag cgc gtc acc ggc acg tgg gaa ttg ggc 3360 Arg Pro Gly Tyr Ile Ala Glu Arg Val Thr Gly Thr Trp Giu Leu Gly 1105 1110 1115 1120 cag ggc tgg gct gac cac gtg gtc gct gaa gtt gtg atc ggc gcc cgc 3408 Gin Gly Trp Ala Asp His Val Val Ala Giu Val Val Ile Gly Ala Arg 1125 1130 1135 gaa ggc gca tcc ctg cgc ggc ggc gac ctg gcg tca ctg tct cct gca 3456 Giu Gly Ala Ser Leu Arg Gly Gly Asp Leu Ala Ser Leu Ser Pro Ala 1140 1145 1150 agc cca gcg tct gca tca gat ctt gat tcg ctt atc gac gca gcc gtc 3504 Ser Pro Ala Ser Ala Ser Asp Leu Asp Ser Leu Ile Asp Ala Ala Val 1155 1160 1165 cag gcc gta gcc tcc cgc cgc ggc gtt gcg gtc tcc ctg cct tca gca 3552 Gin Ala Val Ala Ser Arg Arg Gly Val Ala Val Ser Leu Pro Ser Ala 1170 1175 1180 ggc ggc gct gcc ggt ggc gtg gtt gat tcc gca gct ctt ggc gag ttt 3600 Gly Gly Ala Ala Gly Gly Val Val Asp Ser Ala Ala Leu Gly Glu Phe 1185 1190 1195 1200 gca gag.cag gtc acc gga cac gat ggt gtg ctt gct cag gca gcc cgc 3648 Ala Giu Gin Val Thr Gly His Asp Giy Val Leu Ala Gin Ala Ala Arg 1205 1210 1215 acc atc ttg acc cag ttg ggt ctt gat aag cca gca acc gtt tcc gtg 3696 Thr Ile Leu Thr Gin Leu Gly Leu Asp Lys Pro Ala Thr Val Ser Val 1220 1225 1230 gaa gac acc gca gag gaa gac ctc tac gag ttg gtc tcc aag gaa ctc 3744 Giu Asp Thr Ala Giu Giu Asp Leu Tyr Glu Leu Val Ser Lys Giu Leu 1235 1240 1245 ggt tct gat tgg cca cgt cag gtt gca cca agc ttc gat gaa gaa aag 3792 Gly Ser Asp Trp Pro Arg Gin Val Ala Pro Ser Phe Asp Glu Glu Lys 1250 1255 1260 gtt gtt ctg ctt gat gac cgt tgg gct tct gcg cgt gag 3831 Val Val Leu Leu Asp Asp Arg Trp Ala Ser Ala Arg Glu 1265 1270 1275 WO 01/00805 <210> 54 <211> 1277 <212> PRT <213> Corynebacterium glutamicum PCTIBOO/00926 <400> 54 Gin Trp Leu Asn 1 Trp Ala Le u Ala Leu Lys Asp Al a Asp 145 Arg Trp Asp Ala Le u 225 Phe Gin Ile Glu Thr Ala Cys Asp Asp Val 130 Arg Ser Ala L ys As n 210 Ala Thr Glu Ala Arg Giu Asp Thr Arg 100 Tyr Gi y ValI Asp Arg 180 Ser Val1 Ser Pro Ala 260 Arg 5 Ser Leu Asp Leu Pro Arg Thr Ile Asp Ile 165 As n Arg Tyr Th r Ile 245 Giu Tyr Leu Glu Leu Trp Ile Gi y Leu Gly Trp Al a Thr Al a 150 Met Ile Ser Glu Al a 230 Asp Al a Ala Giu Val1 55 Thr Lys Trp Asp Lys 135 Thr Gly Pro Giu Asp 215 Phe Ala Ala Ala Gin Asp Pro Pro Arg Gin 120 Ala Ilie Lys Ser Ph e 200 Al a Gly Le u Met Pro Gin Gin Ala Ala Val Le u Ile ValI 140 Asp Ser Ser His Met 220 Glu Val1 Th r ValI Met Phe Ala T rp Pro Trp Pro 125 Ala Gi u Pro Le u Se r 205 Le u Le u Pro Arq Asp Leu Giu Tyr Phe Val1 Gin 110 Gly Asp His Gly Gi y 190 Pro Th r Lys Leu Ile 270 Gly Giu Pro Pro Le u Ile Se r Val1 Leu Asp Thr 175 His Thr Val Ile Val 255 Ala Gly Gly Thr Leu Ala Thr Val Asn Asn Gly Thr Ala Thr Trp Giu Thr WO 01/00805 PCT/IB00/00926 Ser Val Asp Ala Gly Val Ile Ala Asp Tyr Asn Asn Val Thr Ala Gly 290 295 300 Tyr Leu Pro Ala Ser Val Val Pro Ala His Thr Ala Pro Asp Val Leu 305 310 315 320 Val Gly Arg Ala Trp Pro Ala Val Phe Ala Ala Val Lys Ser Ala Val 325 330 335 Ile Pro Gly Thr Asp Ser Ala Ser Val Val Glu Gly Met Leu Ser Leu 340 345 350 Val His Leu Glu His His Ile Val Leu Lys Ser Asp Val Pro Thr Asp 355 360 365 Gly Ala Leu Lys Val Ser Ala Thr Ala Asp Glu Val Val Asp Thr Asp 370 375 380 Leu Gly Arg Leu Val Ile Val Arg Ala Glu Ile Ala Asp Ala Glu Gly 385 390 395 400 Asn Leu Ile Ala Thr Leu Ala Glu Arg Phe Ala Ile Arg Gly Arg Lys 405 410 415 Gly Asn Ala Val Ala Arg Thr Asn Thr Ser Ala Leu Pro Thr Thr Val 420 425 430 Asp Thr Pro Arg Ser Ala Arg Ala Val Ala Thr Val Val Ala Pro Glu 435 440 445 Ser Met Arg Pro Phe Ala Val Ile Ser Gly Asp Arg Asn Pro Ile His 450 455 460 Val Ser Asp Val Ala Ala Ser Leu Ala Gly Leu Pro Gly Val Ile Val 465 470 475 480 His Gly Met Trp Thr Ser Ala Ile Gly Glu Leu Ile Ala Gly Ala Ala 485 490 495 Phe Asn Asp Glu Gin Ile Gin Thr Pro Ala Ala Lys Val Val Glu Tyr 500 505 510 Thr Ala Thr Met Leu Ala Pro Val Leu Pro Gly Glu Glu Ile Glu Phe 515 520 525 Ser Val Glu Arg Ser Ala Val Asp Asn Arg Pro Gly Met Gly Glu Val 530 535 540 Arg Thr Val Thr Ala Thr Val Asn Gly Asn Leu Val Leu Thr Ala Thr 545 550 555 560 Ala Val Val Ala Ala Pro Ser Thr Phe Tyr Ala Phe Pro Gly Gin Gly 565 570 575 Ile Gin Ser Gin Gly Met Gly Met Glu Ala Arg Arg Asn Ser Gin Ala 580 585 590 Ala Arg Ala Ile Trp Asp Arg Ala Asp Ala His Thr Arg Asn Lys Leu 595 600 605 Gly Phe Ser Ile Val Glu Ile Val Glu Asn Asn Pro Arg Glu Val Thr WO 01/00805 610 Val Ala Gly G: 625 Gin Phe Thr G' Giu Met Arg G.

61 His Ser Val G' 675 Ser Leu Glu Sf 690 His Arg Leu Vi 705 Ala Ala Leu Ai Asp Tyr Val

A:'

7' Val Asn Tyr A., 755 Ala Gly Leu A: 770 Gin Arg Ala P) 785 Ser Lys Leu A: Leu Ilie Pro A.

8: Pro Asn Leu V.

835 Ser Met Ala G.

850 Asp Phe Lys A.

865 Leu Ilie Glu Lb Glu Thr Gin A: 91 Glu Vai Gly V 915 Thr Leu Arg D 930 PCTfIB00100926 615 Lys Phe Phe His Pro Asp Val 645 Ala Giu Val1 Asp Pro 725 Se r Le u Al a Ile Asp 805 Gi u Ala Val1 Al a Le u 885 Leu Gi y Pro 630 Gly His Tyr Leu Arg 710 Asn ValI Al a Leu Le u 790 Gi y Le u Arg Val1 Ser 870 Ala Le u Se r Gin Met Al a Asn Giu 695 Asp Lys Ser Gly Arg 775 Ile Val Asp Pro Giu 855 Al a T rp Ile Ala Tyr 935 Al a Leu Al a 680 Ile Gi u Met Glu Leu 760 Al a Pro Gly Leu Phe 840 Ser Asp Gin Lys Pro 920 Ala Thr As n 665 Leu Val Asn Gly Ala 745 Gin Asp Gi y Al a Asp 825 Glu Thr Lys Phe Gly 905 Th r Asp Leu 650 Gin Ala T yr Gly Leu 730 Ser Tyr Val1 Ile Phe 810 Val1 Le u Tyr Gin Al a 890 Leu Le u Ala 620 'Val1 Val Al a Tyr Arg 700 Se r Ala Giu Val1 Asn 780 Val1 Gi u Val1 Giu Asn 860 Leu Pro Al a As n Ile 940 Al a Tyr Al a 685 Gly Asn Asp Phe Al a 765 Arg Pro His Gly Giu 845 Glu Ala Val Giu Met 925 Glu Leu Tyr Leu Thr Gin Phe 670 Gly Le u Tyr As n Le u 750 Gi y Al a The Le u Arg 830 Phe Ile Arg Arg Arg 910 Met ValI 640 Ile Ala 655 Ala Gly Val Leu Thr Met Ala Leu 720 Val Phe 735 Glu Ile Thr Gin Pro Gly His Ser 800 Asp Ser 815 Tyr Ilie Val Ala Leu Ala Thr Leu 880 Trp Ile 895 Phe Val Gly Gin Leu Asn WO 01/00805 PCT/IB00/00926 Ile Glu Arg Asp Arg Pro Val Val Phe Ala Thr Asp Glu Val Val Arg 945 950 955 960 Glu Val Ala Val Glu Glu Thr Pro Ala Ala Pro Ala Glu Thr Thr Glu 965 970 975 Thr Pro Ala Thr Pro Ala Thr Pro Ala Pro Val Ala Ala Ala Ala Pro 980 985 990 Ala Thr Gly Gly Pro Arg Pro Asp Asp Ile Ser Phe Thr Pro Ser Asp 995 1000 1005 Ala Thr Glu Met Leu Ile Ala Ile Trp Thr Lys Val Arg Pro Asp Gin 1010 1015 1020 Met Gly Ala Thr Asp Ser Ile Glu Thr Leu Val Glu Gly Val Ser Ser 1025 1030 1035 1040 Arg Arg Asn Gin Leu Leu Leu Asp Leu Gly Val Glu Phe Gly Leu Gly 1045 1050 1055 Ala Ile Asp Gly Ala Ala Asp Ala Glu Leu Gly Asp Leu Lys Val Thr 1060 1065 1070 Val Ser Lys Met Ala Lys Gly Tyr Lys Ala Phe Gly Pro Val Leu Ser 1075 1080 1085 Asp Ala Ala Ala Asp Ala Leu Arg Arg Leu Thr Gly Pro Thr Gly Lys 1090 1095 1100 Arg Pro Gly Tyr Ile Ala Glu Arg Val Thr Gly Thr Trp Glu Leu Gly 1105 1110 1115 1120 Gin Gly Trp Ala Asp His Val Val Ala Glu Val Val Ile Gly Ala Arg 1125 1130 1135 Glu Gly Ala Ser Leu Arg Gly Gly Asp Leu Ala Ser Leu Ser Pro Ala 1140 1145 1150 Ser Pro Ala Ser Ala Ser Asp Leu Asp Ser Leu Ile Asp Ala Ala Val 1155 1160 1165 Gin Ala Val Ala Ser Arg Arg Gly Val Ala Val Ser Leu Pro Ser Ala 1170 1175 1180 Gly Gly Ala Ala Gly Gly Val Val Asp Ser Ala Ala Leu Gly Glu Phe 1185 1190 1195 1200 Ala Glu Gin Val Thr Gly His Asp Gly Val Leu Ala Gin Ala Ala Arg 1205 1210 1215 Thr Ile Leu Thr Gin Leu Gly Leu Asp Lys Pro Ala Thr Val Ser Val 1220 1225 1230 Glu Asp Thr Ala Glu Glu Asp Leu Tyr Glu Leu Val Ser Lys Glu Leu 1235 1240 1245 Gly Ser Asp Trp Pro Arg Gin Val Ala Pro Ser Phe Asp Glu Glu Lys 1250 1255 1260 WO 01/00805 PCTIIBOO/00926 Val Val Leu Leu Asp Asp Arg Trp Ala Ser Ala Arg Glu 1265 1270 1275 <210> <211> 4953 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(4930) <223> RXA02582 <400> tggatgtcta ttctcctc.ag cctcgttttg, gttgaggcgg atagttttaa tattttttta agtttaagtt gtaatcgagc tgaaaggctg aggcctcaat atg gaa Met Glu t cg Se r tgg Trp aag Lys tct Se r get Al a ggc Gi y gtg Val1 geg Ala gte ValI 135 ga t Asp gtt Val1 g ca Ala ggt Gi y tat Tyr gag Glu a gc Ser eg t Arg 120 gag Glu ca g Gln gte Val1 atg Met gag Giu gag Glu cc t Pro ga t Asp 105 tac Tyr gg C Gly ace Thr a eg Th r ttt Phe aa c As n 60 a cg Th r ge a Al a gg t Gly get Al a gg t Gly 140 gtt Val ggt Gly gge Gly 45 ct g Le u atc Ile cat His tee Se r gag Giu 125 ate Ile g aa Giu att Ile 30 ett Leu etg Leu egt Arg ace Thr cac His 110 age Se r t eg Ser ca g Gin ceg Pro tee Se r gac Asp agt Ser eag Gin ga t Asp ctg Le u gat Asp gtt Val1 Val1 tct Se r ace Thr ttg Leu eg t Arg att Ile gag Giu eta Leu 1 eg C Arg gag Glu eg C Arg tee Se r geg Aia gaa Gi u g eg Ala gat Asp eeg Pro 145 ace Th r ga a Giu ga t Asp ttg Le u ca g Gin ttg Leu gtt Val1 atg Met 130 att Ile eag age eaa Gin Ser Gin tgg etc egt Trp Leu Arg gtt aeg gat Val Thr Asp gtt gtt gtg Val Val Vai gat gee ace Asp Ala Thr ege ctt gtt Arg Leu Val aat tte tee Asn Phe Ser 100 gte ggt atg Val Gly Met 115 tgg aag eta Trp Lys Leu ggc cgt tgg Gly Arg Trp tet gag tat gca Ser Glu Tyr Ala 150 ggc gat gag Gly Asp Giu 155 gtt atg tet egg Vai Met Ser Arg 160 aag atg gaa gag ttt Lys Met Glu Glu Phe 165 tct Se z ttc Phe cg t Arg 9 ca Al a t cc Se r 230 cat His att Ile g ca Ala cg t Ar 9 gca Ala 310 cca Pro cg t Arg atc Ile aac As n cag Gin 390 acc WO 01/00805 acc atc g Thr Ile G.

ttt ggt ci Phe Gly L 11 att ttg ci Ile Leu L 200 cca aac ac Pro Asn TI 215 *aac aac g~ *Asn Asn A~ cct tat gC Pro Tyr A aac tac g( Asn Tyr A tgt tct tc Cys Ser Sf 280 aat ggc g~ Asn Gly G: 295 tct cca t Ser Pro P1 acc ggc ac Thr Gly L~ tcc gac gc Ser Asp G: 3' cgc gac gc Arg Asp G 360 tcc gat gc Ser Asp G 375 gtt gat g1 Val Asp V acc gtg g~ PCT/IBOO/00926 atc tct agc ttt gat gcg gag 643 Ile Ser Ser Phe Asp Ala Giu 175 180 gt ggc Gly 170 tg tct eu Ser 85 :g gag eu Giu :t ttg hr Leu at tac sp Tyr :g ctg la Leu 250 :t ttc la Phe ct tct ?r Ser aa gcg Lu Ala :t gtc he Val ag atc y's Ile 330 )C 9CC ly Ala 45 Ic gac Ly Asp )t cac Ly His tg ctg al Leu at tac tac ctg tca Tyr Leu Ser ctc Le u acg Thr ggc Gly 220 atg Met ggt Gi y ttc Phe gta Val1 cac His 300 act Thr gca Al a gtc Val1 atc Ile aac Asn 380 cg t Arg gag gag Giu tgg T rp 205 gaa Giu atg Met act Thr cgc Arg g cg Ala 285 gc t Ala 9 ca Ala ttc Ph e gt t Val atc Ile 365 gga Gly gca Ala gct gat Asp gcc Ala 190 gag Giu gcc Al a atc Ile tct Ser gg t Gi y 270 gtt Val1 atc Ile ttc Phe tct Ser gtg Val1 350 ggt Gly ctg Leu tat Tyr cac gat Asp tac T yr 210 ttc Phe cca Pro gtc Val aac As n gtc Val1 290 gtg Val ggt Gly gat Asp gtg ValI gg t Giy 370 aac As n cag Gin acc cag Gin cgc Arg ggt Gly gaa Glu aac Asn 260 gat Asp g cg Ala att Ile atc Ile ttc Phe 340 gac Asp gca Al a ga c Asp ga t Asp ctg ca 9 Gin atc Ile tcc Se r gca Al a 245 cg c Arg a cc Thr ctg Le u ttg Le u agc Ser 325 gtg Val1 9 ca Ala g tg Val 9 ca Ala ccc Pro 405 ggt 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 WO 01/00805 PCT/IBOO/00926 Thr Thr Val Asp Tyr Val Glu Ala His Gly Thr Gly Thr Ile Leu Gly 410 415 420 qac ccc att gaa gca act gcc ctg ggt gct gtt ttg ggc tat ggc cgt 1411 Asp Pro Ile Giu Ala Thr Ala Leu Gly Ala Val Leu Gly Tyr Gly Arg 425 430 435 gac gca tcc acc cca act ctt ctg ggc tct gca aag tcc aac ttc ggc 1459 Asp Ala Ser Thr Pro Thr Leu Leu Gly Ser Ala Lys Ser Asn Phe Gly 440 445 450 cac act gag tca gct gcg ggt att gca ggt gtc atc aag gtg ctg ctt 1507 His Thr Giu Ser Ala Ala Gly Ile Ala Gly Val Ile Lys Vai Leu Leu 455 460 465 gct ttg cag aac aag act ctg cca cca acc gtg aac ttt gca ggt cca 1555 Ala Leu Gin Asn Lys Thr Leu Pro Pro Thr Val Asn Phe Ala Gly Pro 470 475 480 485 aac cgc tac atc gat ttc gat gct gag cgt ttg gaa gtt gtg gaa gat 1603 Asn Arg Tyr Ile Asp Phe Asp Ala Giu Arg Leu Giu Vai Val Giu Asp 490 495 500 cca cgt gaa tgg ccg gaa tac aac ggt cac gca gta gct ggt gtg tct 1651 Pro Arg Glu Trp Pro Giu Tyr Asn Gly His Ala Val Ala Gly Val Ser 505 510 515 gcg ttc ggg ttc ggt gga acc aac gcg cac gtg gtg atc tct gag tac 1699 Ala Phe Gly Phe Gly Gly Thr Asn Aia His Val Val Ile Ser Giu Tyr 520 525 530 aac gca gaa gac tac gaa acc cgt gcc ccg aag gag gcq ttg ctt cct 1747 Asn Ala Giu Asp Tyr Giu Thr Arg Ala Pro Lys Giu Ala Leu Leu Pro 535 540 545 gac cag cag gtt gcc ctg ccg gtg tct ggt cac ctg cca tcc agg cgt 1795 Asp Gin Gin Val Ala Leu Pro Val Ser Gly His Leu Pro Ser Arg Arg 550 555 560 565 cga caa gca gct gct gac ttg gcg gac ttc ttg gag ggc cgc aaa gat 1843 Arg Gin Ala Ala Ala Asp Leu Ala Asp Phe Leu Giu Gly Arg Lys Asp 570 575 580 tgc gac cta acc cca gta gcc cgc gcg ctg gca ggc cgc aat cat ggc 1891 Cys Asp Leu Thr Pro Val Ala Arg Ala Leu Ala Gly Arg Asn His Gly 585 590 595 cgc tcc cgc gca gtc gtg ctt gca agc act atc gag gaa gcc gta aag 1939 Arg Ser Arg Ala Val Val Leu Ala Ser Thr Ile Giu Glu Ala Val Lys 600 605 610 cgc ctg cgc cag gtc gca gaa ggc aag gtc agc gtc ggt atc tcc gca 1987 Arg Leu Arg Gin Val Ala Giu Gly Lys Val Ser Val Gly lie Ser Ala 615 620 625 gct gat tcc cca gcg gcc aac ggt cct gtg ttt gtg tac tcc ggt ttc 2035 Ala Asp Ser Pro Ala Ala Asn Gly Pro Val Phe Val Tyr Ser Gly Phe 630 635 640 645 gga tcc cag cac cgc ctc atg atc aag gaa ttg tgc tca att tcg cca 2083 Gly Ser Gin His Arg Leu Met Ile Lys Giu Leu Cys Ser Ile Ser Pro WO 01/00805 WO 0100805PCTJIBOO/00926 cag Gin t cc Se r gac Asp act Th r 710 atg Met ga c Asp.

gag Giu gtg Val gag G lu 790 acc Thr gaa Giu cac His gct Ala gat Asp 870 ttc cgc Phe Arg ggc tgg Gly Trp 680 acc gag Thr Glu 695 gac ctg Asp Leu tcg atg Ser Met cgc gat Arg Asp ggc gag Gly Giu 760 gaa ttc Giu Phe 775 tac aag Tyr Lys gtg ggt Val Gly tct gaa Ser Glu acc tct Thr Ser 840 ggc atc Gly Ilie 855 cag ggt Gin Gly gag cgc Giu Arg 665 tcc atc Ser Ile acc gcg Thr Ala ttg gca Leu Ala ggt gag Gly Glu 730 acc atg Thr Met 745 aag tcc Lys Ser gcc gct Ala Ala ggc att.

Gly Ile gga cct Gly Pro 810 gac aag Asp Lys 825 gct gtg Ala Val gag cca Glu Pro gtt acc Val Thr atc Ile atg Met cag Gin agc Ser 715 atc Ile ctc Le u ctg Le u gcg Ala gag Giu 795 cgc Arg ttt Phe gaa Giu ctt Leu tac Tyr 875 gaa Giu aaa Lys gtt Val1 700 ttc Ph e gct Al a att Ile gct Ala gac Asp 780 cct Pro gac Asp gcc Ala cca Pro ccg Pro 860 cca Pro gag Giu ct t Leu 685 g tg Val1 ggt Gly 9C Ala gcc Ala gag Giu 765 ctg Leu gct.

Ala gcc Al a cg c Arg ctt Leu 845 ctg Le u gtt Val1 ctc Leu 670 gtt Val1 atc Ile gta Val1 gct.

Al a agc Se r 750 ga t Asp gat Asp gtt Val1 gtg Val1 ctg Leu 830 ctt Leu ca g Gin gga Gly gat gaa Asp Giu ttg gat Leu Asp acc gca Thr Ala aag cca Lys Pro 720 tac gct Tyr Ala 735 cac cgt His Arg cag ctg Gin Leu aag ttc Lys Phe tat gca Tyr Ala 800 gtt cag Val Gin 815 ctC aac Leu Asn ggt gag Gly Giu atc ccg Ile Pro gca gtg Ala Val 880 gtg tac Val Tyr 895 atg Met ga t Asp atc Ile 705 gct.

Al a gcc Ala tcc Ser gg t Gly atc Ile 785 ggc Gly ttc Phe gtc Val1 ctc Le u ttg Le u 865 gtc Val1 ttc Phe gt t Val gag Gi u 690 ca g Gin gcc Al a gg t Gly cg c Arg cc Al a 770 gaa Giu cca Pro gta Val1 a ag Lys gct.

Ala 850 ttC Phe cac His cag Gin aag Lys 675 caa Gin atc Ile gtc Val1 gga Gly cta Le u 755 atg Met ga a Glu gg C Gly gag Giu Gly 835 ggc Gly agc Ser gac Asp ga C Asp ttC Ph e act Thr gca Ala atg Met ctc Leu 740 atg Met gct Ala aac Asn atg Met aag Lys 820 gct Ala gaa Glu tct Se r g cc Ala tcc Se r 900 gag Glu tac T yr ctg Leu ggc Gly 725 agc Ser ggC Gi y gtg Val cct Pro act Th r 805 ctg Le u ggc Gly atc Ile gt c Val gat Asp 885 ac Thr 2131 2179 2227 2275 2323 2371 2419 2467 2515 2563 2611 2659 2707 2755 2803 tac atg Tyr Met ctc cgc tgt acc cgc cag tcc Leu Arg Cys 890 Thr Arg Gin Ser WO 01/00805 WO 0100805PCTIIBOO/00926 gaa gct gca ttc gct gca ggc cac aac acc ttg qtg gaa att tcc ccg 2851 Glu Ala Ala Phe Ala Ala Gly His Asn Thr Leu Val Giu Ile Ser Pro 905 910 915 aac cca gtt gca ctc atg ggc atg atg aac acc gcg ttc acc gtg ggc 2899 Asn Pro Val Ala Leu Met Gly Met Met Asn Thr Ala Phe Thr Val Gly 920 925 930 aag cct gat gca cag ctg ctg ttc agc ctg aag cga aaa gtc cca gag 2947 Lys Pro Asp Ala Gin Leu Leu Phe Ser Leu Lys Arg Lys Val Pro Giu 935 940 945 gca gaa tcc ctc cgc gac ctt ctg gcg aag ctg tac gtc aac ggc gca 2995 Ala Giu Ser Leu Arg Asp Leu Leu Ala Lys Leu Tyr Val Asn Giy Ala 950 955 960 965 aac gtt gat ttc tcc gcg ctg tac ggc gaa ggt gaa acc atc gat ccg 3043 Asn Val Asp Phe Ser Ala Leu Tyr Gly Giu Gly Glu Thr Ile Asp Pro 970 975 980 cca cac atc acc tgg aaq cac cag cgc ttc tgg act tcc gca cgc cca 3091 Pro His Ile Thr Trp Lys His Gin Arg Phe Trp Thr Ser Ala Arg Pro 985 990 995 tcc tct ggc gca tcc ctg gat ctg ccg ggc ttc cgc gtq aat ctg cca 3139 Ser Ser Gly Ala Ser Leu Asp Leu Pro Gly Phe Arg Val Asn Leu Pro 1000 1005 1010 aac aac act gtq gcg ttt tcc acc gca gcc gaa ctg gca cca tcc gca 3187 Asn Asn Thr Val Ala Phe Ser Thr Ala Ala Glu Leu Ala Pro Ser Ala 1015 1020 1025 gtg gca atc atg gaa gca gcc gcc atg gct gtc acc cca ggt tcc tcc 3235 Val Ala Ile Met Glu Ala Ala Ala Met Ala Val Thr Pro Gly Ser Ser 1030 1035 1040 1045 gtc gac gca gtg gac gag cgc gac atg ctg cca cca aqc gqc gaa atc 3283 Val Asp Ala Val Asp Glu Arg Asp Met Leu Pro Pro Ser Gly Glu Ile 1050 1055 1060 acc acc atc gtg act cgc tcc ttg ggc ggt ttg agc ctg tcc gta tac 3331 Thr Thr Ile Val Thr Arg Ser Leu Gly Gly Leu Ser Leu Ser Val Tyr 1065 1070 1075 aag att gag gga acc acc agc act ctt gtc gcc gaa ggt ttc gca gcc 3379 Lys Ile Glu Giy Thr Thr Ser Thr Leu Val Ala Giu Gly Phe Ala Ala 1080 1085 1090 aac cca gga ttc gcc gca gca tct tcc ttc gac ggt cca ggc tac gac 3427 Asn Pro Gly Phe Ala Ala Ala Ser Ser Phe Asp Gly Pro Gly Tyr Asp 1095 1100 1105 gga ttc aac acc gat tac agc gac cag cca gac cct cgc tcc gac ctg 3475 Gly Phe Asn Thr Asp Tyr Ser Asp Gin Pro Asp Pro Arg Ser Asp Leu 1110 1115 1120 1125 cct ttg gac atc gaa gca gtc cgc tgg gac cca gcg acc gaa acc gtc 3523 Pro Leu Asp Ile Giu Ala Val Arg Trp Asp Pro Ala Thr Glu Thr Val 1130 1135 1140 WO 01/00805 PCTIBOO/00926 gaa gag cgc atg cgc gcc atc gtc tcc gaa gca atg ggc tac gac gtg 3571 Glu Giu Arg Met Arg Ala Ile Vai Ser Giu Ala Met Gly Tyr Asp Val 1145 1150 1155 gat gac ctc cca cgc gaa ctc cca ctg att gac ctc ggc ctc gac tcc 3619 Asp Asp Leu Pro Arg Giu Leu Pro Leu Ile Asp Leu Gly Leu Asp Ser 1160 1165 1170 ctc atg ggc atg cgc atc aaa aac cgc atc gaa aat gac ttc cag atc 3667 Leu Met Gly Met Arg Ile Lys Asn Arg Ile Giu Asn Asp Phe Gin Ile 1175 1180 1185 cca cca ctc cag gtc caa gcg ctc cgc gat gca tcc gtc gct gac gtg 3715 Pro Pro Leu Gin Vai Gin Aia Leu Arg Asp Aia Ser Val Aia Asp Val 1190 1195 1200 1205 gta atc atg gtg gaa aac atg gtc gct ggc cgc tcc tct gag acg ctt 3763 Val Ile Met Vai Giu Asn Met Vai Ala Giy Arg Ser Ser Giu Thr Leu 1210 1215 1220 gtc gac gcc acc ccg cag gtg cca gct gag gca gca ggg gag gct caa 3811 Val Asp Aia Thr Pro Gin Val Pro Aia Giu Ala Ala Gly Giu Ala Gin 1225 1230 1235 gct gct gag tct tct gct tcg ggc gag gac gtg cag ggc gtt ggc gtt 3859 Ala Ala Giu Ser Ser Ala Ser Giy Glu Asp Vai Gin Gly Vai Gly Vai 1240 1245 1250 gca ccg cga gat gcg tcg gaa cgc atg gtc ttt ggt act tgg gca ggc 3907 Ala Pro Arg Asp Ala Ser Giu Arg Met Val Phe Giy Thr Trp Ala Gly 1255 1260 1265 ctg act ggc gct gcg gca gct ggc gtg acc agc aag ttg cca cag atc 3955 Leu Thr Gly Ala Ala Ala Ala Gly Val Thr Ser Lys Leu Pro Gin Ile 1270 1275 1280 1285 gat gtc gat act gca acc gca att gcc gag cgc ctc acc gag cgt tcc 4003 Asp Vai Asp Thr Ala Thr Ala Ile Ala Glu Arg Leu Thr Giu Arg Ser 1290 1295 1300 gga att gaa atc agc act gaa cag gtg ttg gct gca gaa acc ctc gaa 4051 Gly Ile Giu Ile Ser Thr Giu Gin Val Leu Ala Ala Giu Thr Leu Glu 1305 1310 1315 cca ctg tct gac ctg gtg cgt gaa ggc ctc gaa act gaa gtt cag ggc 4099 Pro Leu Ser Asp Leu Vai Arg Giu Gly Leu Giu Thr Giu Val Gin Gly 1320 1325 1330 aac att cgt gtg ctg cgt gga cgt gca gaa ggc tcc acc aag cct gca 4147 Asn Ile Arg Val Leu Arg Gly Arg Ala Giu Gly Ser Thr Lys Pro Ala 1335 1340 1345 gtg ttc atg ttc cac cca gct ggc ggt tct tcc gtg gtc tac caa cca 4195 Val Phe Met Phe His Pro Ala Gly Gly Ser Ser Val Vai Tyr Gin Pro 1350 1355 1360 1365 cta atg cgt cgt ctg cct gaa gat gtc cct gtc tat ggc gtt gag cgt 4243 Leu Met Arg Arg Leu Pro Giu Asp Val Pro Val Tyr Gly Val Glu Arg 1370 1375 1380 ctg gaa ggc gat ctc gct gac cgc gcg gca gca tat gtt gat gac atc 4291 WO 01/00805 PCTLBOOIOO926 Leu Glu Gly Asp Leu Ala Asp Arg Ala Ala Ala Tyr Val Asp Asp Ile 1385 1390 1395 aag aag tac tcc gat ggc ttc cca gta gtt ttg ggt ggc tgg agc ttc 4339 Lys Lys Tyr Ser Asp Gly Phe Pro Val Val Leu Gly Gly Trp Ser Phe 1400 1405 1410 ggc ggt gcc gtt gcc ttc gag gtt gcc cac caa ctg gtt ggc tcc gat 4387 Gly Gly Ala Val Ala Phe Giu Val Ala His Gin Leu Val Gly Ser Asp 1415 1420 1425 gtt gag gta gct acc gtg gcg ttg ctg gat act gtg cag cct tca aac 4435 Val Giu Val Ala Thr Val Ala Leu Leu Asp Thr Vai Gin Pro Ser Asn 1430 1435 1440 1445 cca gca cca gat acc gct gag gaa act cgt gca cgc tgg act cgc tac 4483 Pro Ala Pro Asp Thr Ala Giu Giu Thr Arg Ala Arg Trp Thr Arg Tyr 1450 1455 1460 gcg gac ttc gcc aag aag acc tac ggc ctt gat ttc gag gta cct ttt 4531 Ala Asp Phe Ala Lys Lys Thr Tyr Gly Leu Asp Phe Giu Val Pro Phe 1465 1470 1475 gaa atc ttg gac acc atc ggt gaa gac gga atg ctg tcc atg atg acc 4579 Glu Ile Leu Asp Thr Ile Gly Glu Asp Gly Met Leu Ser Met Met Thr 1480 1485 1490 gac ttc ctg gcc aac acc gac gcc tcc gag cac gga ttg tcc gca ggt 4627 Asp Phe Leu Ala Asn Thr Asp Ala Ser Glu His Gly Leu Ser Ala Gly 1495 1500 1505 gtg ctt gaa cac cag cgc gct tct ttt gtg gac aac cgc atc ctg gct 4675 Val Leu Giu His Gin Arg Ala Ser Phe Val Asp Asn Arg Ile Leu Ala 1510 1515 1520 1525 aaa ctt aat ttt gca gac tgg gcc aac gtt gaa gcc cct gtc atc ctg 4723 Lys Leu Asn Phe Ala Asp Trp Ala Asn Val Glu Ala Pro Val Ile Leu 1530 1535 1540 ttc cgc gcg gaa cgc atg cat gat gga gct atc gaa ctt gaa cca aac 4771 Phe Arg Ala Giu Arg Met His Asp Gly Ala Ile Giu Leu Glu Pro Asn 1545 1550 1555 tat gcc aag att gat caa gat gga gga tgg tcc gga att gtc aac gat 4819 Tyr Ala Lys Ile Asp Gin Asp Gly Gly Trp Ser Gly Ile Val Asn Asp 1560 1565 1570 ttg gaa att gtt cag ctg aat ggt gac cac ctg gca gtt gtc gat gaa 4867 Leu Glu Ile Vai Gin Leu Asn Gly Asp His Leu Ala Val Val Asp Giu 1575 1580 1585 cca gaa atc ggc aca gtc gga gct cat ttg agt cgc cgc att gat gag 4915 Pro Giu Ile Gly Thr Val Gly Ala His Leu Ser Arg Arg Ile Asp Giu 1590 1595 1600 1605 att tct cgg aag aat tagtaacgga gagctgacgg aag 4953 Ile Ser Arg Lys Asn 1610 <210> 56 WO 01/00805 <211> 1610 <212> PRT <213> Corynebacterium glutamicum <400> 56 Met Giu Gin Ser Gin Ser Ser Asp Gin PCT/IBOO/00926 1 Arg Glu Ar g Se r Al a Glu Al a Asp Pro 145 Lys Ser Met Glu Val 225 Asp Ilie Val1 Thr Giu Asp Leu Gin Le u Vali Met 130 Ile Met Phe Asp Tyr 210 Phe Pro Vali As n Trp ValI Val1 Asp Arg As n Vai 115 T rp Gly G lu Asp Pro 195 Ala Ile Aia Al a Val 275 Le u Thr Val1 Al a Leu Phe 100 Gly Ly s Arg Glu Ala 180 Gin Arg Gly G lu Asn 260 Asp Trp Lys Ser Ala Gly Val Al a ValI 135 Giu Thr Ph e Ile Pro 215 As n Pro As n Cys Val1 Met Gi u Gi u Pro Asp 105 Tyr Gly Ala Gly Leu 185 Le u Thr Asp Al a Al a 265 Ser Lys Arq Glu Le u Tyr Arg Ser Pro Ar g Gi y Gly 170 Se r Glu Leu Tyr Leu 250 Phe Se r Met Thr Thr Glu Pro Arg Pro Gly Asp Asp 155 Tyr Pro Le u Arg Gly 235 Thr Asp Leu Asp Ala Val Arg Ala Leu Arg Asn Gly Glu Ala Gly 305 Le u Ala Arg Lys Pro 385 Ala Gly Le u Ly s Ilie 465 As n Glu Val ValI Glu 545 Leu Glu Gly WO 01/00805 Val Asn Il Gly Val Il Asp Gly Ph Val Asp As 355 Gly Ser Al 370 Asn Pro As Gin Val As Thr Ilie LE Gly Tyr Gl 435 Ser Asn P1 450 Lys Val LE Phe Ala G~ Val Val G Ala Gly V 515 Ile Ser GL 530 Ala Leu L Pro Ser A: Gly Arg L' 51 Arg Asn H 595 0 Lu is Leu Se r 325 Val1 Al a Val1 Al a Pro 405 Gly Arg Gly Le u Pro 485 Asp Ser Tyr Pro Arg 565 Asp Gly Al a 310 Pro Arg Ile As n Gin 390 Th r Asp Asp His Al a 470 Asn Pro Al a As n Asp 550 Arg Cys Arg Ser Th r Se r Arg Ser 375 Val Thr Pro Al a Thr 455 Leu Arg Arg Phe Al a 535 Gin Gin Asp Ser Pro Gly Asp Asp 360 Asp Asp Val Ile Se r 440 Giu Gin Tyr Giu Gly 520 Glu Gin Ala Le u Arq 600 Ph e Lys Gly 345 Gi y Gi y ValI Asp Glu 425 Thr Se r As n Ile Trp 505 Phe Asp Val Al a Thr 585 Al a Val Thr 315 Ile His 330 Ala Gly Asp Lys His Ser Leu Gin 395 Tyr Val 410 Ala Thr Pro Thr Ala Ala Lys Thr 475 Asp Phe 490 Pro Glu Gly Gly Tyr Giu Ala Leu 555 Ala Asp 570 Pro Val Val Val Thr Ala Val1 Ile Asn 380 Arg Giu Al a Le u Gi y 460 Le u Asp Tyr Thr Thr 540 Pro Leu Al a Leu Ala Phe Val1 Ile 365 Gly Ala Ala Le u Le u 445 Ile Pro Al a As n As n 525 Arg Val1 Al a Argi Ala 605 Phe Ser Vali 350 Gi y Le u Tyr His Gly 430 Gly Ala Pro Giu Gly 510 Ala Ala S er Asp Ala 590 S er Al a Asp 335 Le u Vali Th r Vali Gly 415 Ala Ser Gly Thr Arg 495 His His Pro GI y Ph e 575 Le u Th r PCT/IBOO/00926 Giu 320 Asp L ys Ile Al a Asp 400 Th r Vali Ala ValI Vai 480 Le u Ala Val1 Lys His 560 Le u Ala Ile IGlu Glu Ala Vai Lys Arq Leu Arg Gin Val Ala Glu Gly Lys Val Ser WO 01/00805 PCT/IB00/00926 Val Gly Ile Ser Ala Ala Asp Ser Pro Ala Ala Asn Gly Pro Val Phe 625 630 635 640 Val Tyr Ser Gly Phe Gly Ser Gln His Arg Leu Met Ile Lys Glu Leu 645 650 655 Cys Ser Ile Ser Pro Gin Phe Arg Glu Arg Ile Glu Glu Leu Asp Glu 660 665- 670 Met Val Lys Phe Glu Ser Gly Trp Ser Ile Met Lys Leu Val Leu Asp 675 680 685 Asp Glu Gln Thr Tyr Asp Thr Glu Thr Ala Gin Val Val Ile Thr Ala 690 695 700 Ile Gin Ile Ala Leu Thr Asp Leu Leu Ala Ser Phe Gly Val Lys Pro 705 710 715 720 Ala Ala Val Met Gly Met Ser Met Gly Glu Ile Ala Ala Ala Tyr Ala 725 730 735 Ala Gly Gly Leu Ser Asp Arg Asp Thr Met Leu Ile Ala Ser His Arg 740 745 750 Ser Arg Leu Met Gly Glu Gly Glu Lys Ser Leu Ala Glu Asp Gin Leu 755 760 765 Gly Ala Met Ala Val Val Glu Phe Ala Ala Ala Asp Leu Asp Lys Phe 770 775 780 Ile Glu Glu Asn Pro Glu Tyr Lys Gly Ile Glu Pro Ala Val Tyr Ala 785 790 795 800 Gly Pro Gly Met Thr Thr Val Gly Gly Pro Arg Asp Ala Val Val Gin 805 810 815 Phe Val Glu Lys Leu Glu Ser Glu Asp Lys Phe Ala Arg Leu Leu Asn 820 825 830 Val Lys Gly Ala Gly His Thr Ser Ala Val Glu Pro Leu Leu Gly Glu 835 840 845 Leu Ala Gly Glu Ile Ala Gly Ile Glu Pro Leu Pro Leu Gin Ile Pro 850 855 860 Leu Phe Ser Ser Val Asp Gin Gly Val Thr Tyr Pro Val Gly Ala Val 865 870 875 880 Val His Asp Ala Asp Tyr Met Leu Arg Cys Thr Arg Gin Ser Val Tyr 885 890 895 Phe Gin Asp Ser Thr Glu Ala Ala Phe Ala Ala Gly His Asn Thr Leu 900 905 910 Val Glu Ile Ser Pro Asn Pro Val Ala Leu Met Gly Met Met Asn Thr 915 920 925 Ala Phe Thr Val Gly Lys Pro Asp Ala Gin Leu Leu Phe Ser Leu Lys 930 935 940 Arg Lys Val Pro Glu Ala Glu Ser Leu Arg Asp Leu Leu Ala Lys Leu WO 01/00805 PCT/IB00/00926 945 950 955 960 Tyr Val Asn Gly Ala Asn Val Asp Phe Ser Ala Leu Tyr Gly Glu Gly 965 970 975 Glu Thr Ile Asp Pro Pro His Ile Thr Trp Lys His Gin Arg Phe Trp 980 985 990 Thr Ser Ala Arg Pro Ser Ser Gly Ala Ser Leu Asp Leu Pro Gly Phe 995 1000 1005 Arg Val Asn Leu Pro Asn Asn Thr Val Ala Phe Ser Thr Ala Ala Glu 1010 1015 1020 Leu Ala Pro Ser Ala Val Ala Ile Met Glu Ala Ala Ala Met Ala Val 1025 1030 1035 1040 Thr Pro Gly Ser Ser Val Asp Ala Val Asp Glu Arg Asp Met Leu Pro 1045 1050 1055 Pro Ser Gly Glu Ile Thr Thr Ile Val Thr Arg Ser Leu Gly Gly Leu 1060 1065 1070 Ser Leu Ser Val Tyr Lys Ile Glu Gly Thr Thr Ser Thr Leu Val Ala 1075 1080 1085 Glu Gly Phe Ala Ala Asn Pro Gly Phe Ala Ala Ala Ser Ser Phe Asp 1090 1095 1100 Gly Pro Gly Tyr Asp Gly Phe Asn Thr Asp Tyr Ser Asp Gin Pro Asp 1105 1110 1115 1120 Pro Arg Ser Asp Leu Pro Leu Asp Ile Glu Ala Val Arg Trp Asp Pro 1125 1130 1135 Ala Thr Glu Thr Val Glu Glu Arg Met Arg Ala Ile Val Ser Glu Ala 1140 1145 1150 Met Gly Tyr Asp Val Asp Asp Leu Pro Arg Glu Leu Pro Leu Ile Asp 1155 1160 1165 Leu Gly Leu Asp Ser Leu Met Gly Met Arg Ile Lys Asn Arg Ile Glu 1170 1175 1180 Asn Asp Phe Gin Ile Pro Pro Leu Gin Val Gin Ala Leu Arg Asp Ala 1185 1190 1195 1200 Ser Val Ala Asp Val Val Ile Met Val Glu Asn Met Val Ala Gly Arg 1205 1210 1215 Ser Ser Glu Thr Leu Val Asp Ala Thr Pro Gin Val Pro Ala Glu Ala 1220 1225 1230 Ala Gly Glu Ala Gin Ala Ala Glu Ser Ser Ala Ser Gly Glu Asp Val 1235 1240 1245 Gin Gly Val Gly Val Ala Pro Arg Asp Ala Ser Glu Arg Met Val Phe 1250 1255 1260 Gly Thr Trp Ala Gly Leu Thr Gly Ala Ala Ala Ala Gly Val Thr Ser 1265 1270 1275 1280 WO 01/00805 PCT/IB00/00926 Lys Leu Pro Gin Ile Asp Val Asp Thr Ala Thr Ala Ile Ala Glu Arg 1285 1290 1295 Leu Thr Glu Arg Ser Gly Ile Glu Ile Ser Thr Glu Gin Val Leu Ala 1300 1305 1310 Ala Glu Thr Leu Glu Pro Leu Ser Asp Leu Val Arg Glu Gly Leu Glu 1315 1320 1325 Thr Glu Val Gin Gly Asn Ile Arg Val Leu Arg Gly Arg Ala Glu Gly 1330 1335 1340 Ser Thr Lys Pro Ala Val Phe Met Phe His Pro Ala Gly Gly Ser Ser 1345 1350 1355 1360 Val Val Tyr Gin Pro Leu Met Arg Arg Leu Pro Glu Asp Val Pro Val 1365 1370 1375 Tyr Gly Val Glu Arg Leu Glu Gly Asp Leu Ala Asp Arg Ala Ala Ala 1380 1385 1390 Tyr Val Asp Asp Ile Lys Lys Tyr Ser Asp Gly Phe Pro Val Val Leu 1395 1400 1405 Gly Gly Trp Ser Phe Gly Gly Ala Val Ala Phe Glu Val Ala His Gin 1410 1415 1420 Leu Val Gly Ser Asp Val Glu Val Ala Thr Val Ala Leu Leu Asp Thr 1425 1430 1435 1440 Val Gin Pro Ser Asn Pro Ala Pro Asp Thr Ala Glu Glu Thr Arg Ala 1445 1450 1455 Arg Trp Thr Arg Tyr Ala Asp Phe Ala Lys Lys Thr Tyr Gly Leu Asp 1460 1465 1470 Phe Glu Val Pro Phe Glu Ile Leu Asp Thr Ile Gly Glu Asp Gly Met 1475 1480 1485 Leu Ser Met Met Thr Asp Phe Leu Ala Asn Thr Asp Ala Ser Glu His 1490 1495 1500 Gly Leu Ser Ala Gly Val Leu Glu His Gin Arg Ala Ser Phe Val Asp 1505 1510 1515 1520 Asn Arg Ile Leu Ala Lys Leu Asn Phe Ala Asp Trp Ala Asn Val Glu 1525 1530 1535 Ala Pro Val Ile Leu Phe Arg Ala Glu Arg Met His Asp Gly Ala Ile 1540 1545 1550 Glu Leu Glu Pro Asn Tyr Ala Lys Ile Asp Gin Asp Gly Gly Trp Ser 1555 1560 1565 Gly Ile Val Asn Asp Leu Glu Ile Val Gin Leu Asn Gly Asp His Leu 1570 1575 1580 Ala Val Val Asp Glu Pro Glu Ile Gly Thr Val Gly Ala His Leu Ser 1585 1590 1595 1600 WO 01/00805 Arg Arg Ilie Asp Glu Ile Ser Arg Lys Asn 1605 1610 <210> 57 <211> 930 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (907) <223> RXA02691 <400> 57 cttagggctt atctgttttc cagccttgct ttttactagg PCTIEBOO/00926 cgctcctgtc ccgcttcaqt caccaaaacc acacccctgg ttatgaccag atcggctaaa atg aac acc atg cct Met Asn Thr Met Pro ga c Asp gaa Gi u aag Lys ctt Leu tat Tyr gcq Al a cac His a tg Met cg C Arg caa Gin ccc Pro cat His cag Gin 9gc Giy tcc Se r t cg Se r gcc Aia gq t Giy 135 Ccqg Pro ga c Asp cag Gin cct Pro gtc Vali Gly ccg Pro gcc Aila 120 ccc Pro ca g Gin ccc Pro cgt Arg atq Met at t Ilie 75 a ag Lys t cc Ser ct a Leu ga t Asp qga Giy cgc Arq att Ilie 45 cct Pro gt t Vali gct Ala ctg Leu qct Al a 125 att Ile cc t Pro ctc Le u gag Glu gag Glu cgg Arq Gi y ttg Leu a gc Se r gat Asp acc Thr gtg ValI gaa Gi u cqc Arg gcg Aila 80 aaa Lys gcc Al a a gg Arg ttc Phe 1 qca Ala gac Asp atc Ile atc Ile att Ile gg t Gi y t cg Se r att Ile ttt Ph e 145 tcc Ser gqc Gly t qc Cys ctg Leu ggt Gly acc Th r ttt Phe ttg Le u 130 gg t Gly aaa Lys ctt Leu acc Thr gaa Glu gat Asp ttc Phe t cc Ser 115 ag t Ser acc Thr ggg Gly aaa Lys acc Thr ga a Glu ctg Leu gtq Val1 100 g ca Ala tct Ser ga t Asp qg Val1 cca Pro cag Gin a ag Lys gtc ValI qc c Al a gag Giu tcc Se r cgc Arg 115 163 211 259 307 355 403 451 499 547 595 gcg gcc Ala Ala 150 cag cac atc acg ttg cgc cgc ctg cgc ttt gga aat ggt cga Gin His Ile Thr Leu Arg Arg Leu 155 Phe Gly Asn Gly Arg 165 WO 01/00805 WO 0100805PCTIIBOO/00926 ccc tat gcc att gac aac gqt tgg tac aac tcc gaa ttc gca cct gac 643 Pro Tyr Ala Ile Asp Asn Gly Trp Tyr Asn Ser Giu Phe Ala Pro Asp 170 175 180 ctg ctq gaa aat gat gtg tac aac tcc gtg tac tcc atc ctg gac cgc 691 Leu Leu Giu Asn Asp Val Tyr Asn Ser Vai Tyr Ser Ile Leu Asp Arg 185 190 195 gtc tat ggc gtc ccc gtc acc cag gcc gag caa acg gtc acc gcc gta 739 Val Tyr Gly Val Pro Val Thr Gin Ala Giu Gin Thr Vai Thr Ala Val 200 205 210 gca gcc gac gaa gac acc gca cgg ctt ctg gac gtc acc ccc ggc gcc 787 Ala Ala Asp Giu Asp Thr Ala Arg Leu Leu Asp Val Thr Pro Gly Ala 215 220 225 cca ctc ctt cgt atc ctt cga cag tca ctt tct ggc gat aag ccc gtg 835 Pro Leu Leu Arg Ile Leu Arg Gin Ser Leu Ser Gly Asp Lys Pro Vai 230 235 240 245 qaa tgg tgc gtt tcc ttg tac cga acc gac cga tat tct tta aaa aca 883 Giu Trp Cys Val Ser Leu Tyr Arg Thr Asp Arg Tyr Ser Leu Lys Thr 250 255 260 ttg gtt aca cgc tcc gaa gat ctc tgacgtqaac ccattttgqt ggc 930 Leu Val Thr Arg Ser Giu Asp Leu 265 <210> 58 <211> 269 <212> PRT <213> Corynebacterium giutamicum <400> 58 Met Asn Thr Met Pro Asp Gin Pro Leu Asn Gin Asp Gly Phe Pro Thr 1 5 10 Ala Ser Lys Gly Vai Giu Pro Asp Asn Leu Pro Asp Arg Val Leu Val 25 Asp Gly Leu Lys Pro Lys His Gin Gin Leu Arg Giu Ile Leu Giu Glu 40 Ile Cys Thr Thr Gin Leu Gin Pro Gly Asp Met Leu Pro Gly Giu Arg 55 Ile Leu Giu Giu Lys Tyr Gly Val Ser Arg Ile Thr Val Arg Arg Ala 70 75 Ile Gly Asp Leu Val Ala Ser Gly Arg Leu Lys Arg Ala Arg Gly Lys 90 Gly Thr Phe Vai Ala His Ser Pro Leu Ile Ser Arg Leu His Leu Ala 100 105 110 Ser Phe Ser Ala Giu Met Ala Ala Gin Lys Leu Ser Ala Thr Ser Arg 115 120 125 Ile Leu Ser Ser Ser Arg Gly Pro Ala Pro Asp Asp Ile Ala Asp Phe 130 135 140 WO 01/00805 Gly Thr Asp Gly Asn Giy Phe Ala Pro 180 Ile Leu Asp 195 Val Thr Ala 210 Thr Pro Gly Asp Lys Pro Ser Leu Lys 260 PCT/IBOO/00926 Arg Arg 165 Asp Arg Val1 Ala Val 245 Th r Al a 150 Pro Le u Val Ala Pro 230 Glu Leu Ala Tyr Leu Tyr Ala 215 Le u Trp Val Leu Gly Tyr Thr Al a 220 Arg Tyr Asp Arg Trp As n Gin 205 Arg Gln Arg Leu Ar g Tyr Ser 190 Ala Le u Ser Th r Leu Asn 175 ValI Glu Le u Leu Asp 255 <210> 59 (211> 1968 (212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1945) <223> RXA00880 <400> 59 tccatgtggt taaagatatg cctaaagatc tgaccaaaaa cacaagtaca gccaaattaa aggaaaggtt qaatttgacc ac Thr gaa Glu gga Gly gcc Ala tca Se r act Th r gcc Al a a ag Lys gac Asp cag Gin gaa Glu ctt Le u cct Pro gag Glu gqa Gi y cgtgactaaa gacgtgacga atg act tca cct aat Met Thr Ser Pro Asn 1 acc atc gga gaa tct Thr Ile Giy Glu Ser aag act cga cct tac Lys Thr Arg Pro Tyr tgg gtg aat gta act Trp Val Asn Val Thr gca aaa gga att att Ala Lys Gly Ile Ile ctg ctg tcc aat act Leu Leu Ser Asn Thr WO 01/00805 WO 0100805PCTIiBO00926 tgg Trp cct Pro 105 ga t Asp ttg Leu gg t Gly t tg Le u tgg Trp 185 tac Tyr tac Tyr gcg Al a cac His acc Th r 265 cgt Arg gtc Val cg c Arg gct Ala atc Ile tcc Ser atg Met tca Ser gag Glu 170 ga a Glu acc Th r cac His atc Ile gtt Val1 250 cag Gin tcc Se r cg c Arg atc I le gtg Val1 tac Tyr ggc Gly aag Lys c ct Pro 155 a cc Thr cgc Arg tct Ser tgg T rp gcg Ala 235 ctt Le u tcc Ser cgc Arg aac Asn atg Met 315 gct Ala 95 t ca Ser gCC Ala atc Ile cgc Arg gag Giu 175 acc Thr gg t Gly gtc Val1 tca Se r gtg Val1 255 gat Asp att Ile gct Al a gcc Al a atc Ile ctg Le u att Ile ggt Gly cg c Arg 160 gg c Gly aag Ly s agg Arg cga Arg agg Arg 240 cac His ttc Phe ttg *Leu aat As n ga a Glu 320 t gg Trp t CC Se r acc Th r gaa Gi u 145 att Ile cg c Arg gcc Ala ccg Pro g cg Ala 225 ttg Le u ttg Leu agc Ser ggt Gly gct Al a 305 aag Ly s g cc Ala ca a Gin ga a Giu 130 ga C Asp cta Le u gag Glu gct Ala aa g Lys 210 ctg Le u Ct C Le u gCC Ala acc Thr gtt Val 290 gct Ala gcg Ala g ct Ala att Ile 115 acc Thr gga Gly gag Glu ctt Le u ga c Asp 195 ggc Gly atc Ile acc Thr ttc Phe ctt Le u 275 cca Pro gac Asp g cc Ala gg C Gly 100 gag Giu cc t Pro act Thr atc Ile tct Ser 180 ctg Le u tg c Cys acc Thr ttc Phe gct Al a 260 act Thr cqc Arg ggt Gly att Ile g ca Ala tgg Trp ga t Asp cca Pro aac As n 165 gat Asp gcg Al a gag Glu aat Asn ctt Le u 245 gtc Val t tg Le u gtg Val1 gg C Gly gaa Glu 325 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 WO 01/00805 WO 0100805PCT111BOO/00926 tac Tyr atg Met gct Ala gg g Gly ga a Giu 390 act Thr atc Ile ttc Phe ga c Asp cac His 470 ggc Gly cc a Pro ggC Gly aac As n atg Met cat His gg t Gly 360 gag Giu tat Tyr caa Gin atc Ile gga Gly 440 t gg Trp gtg ValI aac As n gtc Vali ctg Leu 520 aac Asn gct Ala aaa Lys 345 gg c Gly ctg Le u ggt Gly aag Lys aa t As n 425 tat Tyr ttc Phe atc Ile gt t Val1 agc Ser 505 gtg Val1 att Ile ctt Leu 330 gcg Ala ga t Asp ctg Le u ct g Le u atc Ile 410 gaa Gi u tgg T rp aac Asn acc Th r tcc S er 490 cag Gin a cc Thr 9cg Ala ga a Giu c tg Leu 350 gcc Al a cg c Arg gcg Ala ggt Gly atc Ile 430 gaa Gi u Ct 9 Le u aaa Lys atg Met g tg ValI 510 ga t Asp acg Thr ggc cca Gly Pro 335 gtg tac Val Tyr atc acc Ile Thr ggt gtg Gly Val gct gct Ala Ala 400 aag ccg Lys Pro 415 atg cta Met Leu gcc aca Ala Thr ggt gag Gly Giu gat ctg Asp Leu 480 gaa gac Glu Asp 495 ggc gat Gly Asp atg ttg Met Leu gtt tct Val Ser gca gtc Ala Val 560 agc Ser tcc Ser ggt Gi y Gly 385 g ca Al a atg Met aaa Lys gca Al a ct g Leui 465 atc Ile atc Ile gg t Gly aa g Ly s gag Glu 545 aac Asn aag Lys aag Lys ggt Gly 370 atg Met gcg Al a gg t Gly ggc Gly gaa Glu 450 ttg Leu gtg Val1 atc Ile aaa L ys c gg Arg 530 att Ile aac Asn t cc Se r atc Ile 355 tca Se r acc Thr gtg Val1 Gly gag Giu 435 gc c Al a gag Giu acc Thr cgc Arg cca Pro 515 t gg Trp gc t Al a g ct Ala cag Gin 340 cg t Arg gcg Al a atc Ile ga c Asp atg Met 420 a tg Met ctc Leu tct Ser g cg Ala gca Ala 500 ttc Phe aa g Lys act Th r aat Asn gt t Val1 g ca Al a at g Met tac Tyr ttc Phe 405 acc Thr ttg Le u cac His gg a Gly gg c Gly 485 cac His gtt Val1 ctg Leu gat Asp gcc Ala 565 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 cct gca ctg cgt gcg Pro Ala Leu Arg Ala 550 gaa atc cag gat Glu Ile Gin Asp 555 WO 01/00805 PCTIIBO0/00926 acg gtg tct cat tca gag gcg atc aag cgg ttc tac atc ctt gat cgc 1843 Thr Val Ser His Ser Giu Ala Ile Lys Arg Phe Tyr Ile Leu Asp Arg 570 575 580 gac ctg acc gag gaa gcc gac gag ctg acc cca acg ctg aag gtc aag 1891 Asp Leu Thr Glu Glu Ala Asp Giu Leu Thr Pro Thr Leu Lys Val Lys 585 590 595 cgc aac gtt gtt gtt cgc cgt tac gca gac gcc atc gac cac atc tac 1939 Arg Asn Val Val Val Arg Arg Tyr Ala Asp Ala Ile Asp His Ile Tyr 600 605 610 aac cga tgagtaacac agagacccaa ttt 1968 Asn Arg 615 <210> <211> 615 <212> PRT <213> Corynebacterium giutamicum <400> Met Thr Ser Pro Asn Thr Leu Gin Glu Tyr Thr Glu Pro Ala Lys Tyr 1 5 10 Thr Ile Gly Glu Ser Giu Thr Cys Leu Thr Ala Leu Leu Asp Gin Ile 25 Lys Thr Arg Pro Tyr Gly Val Leu Phe Ser Lys Pro Ala Asn Tyr Glu 40 Trp Val Asn Val Thr Ala Lys Glu Phe Gin Asp Glu Vai Phe Ala Val 55 Ala Lys Gly Ile Ile Ser Val Gly Val Glu Gin Gly Asp Arg Val Ala 70 75 Leu Leu Ser Asn Thr Arg Tyr Giu Trp Ala Val Leu Asp Phe Ala Ile 90 Trp Ala Ala Gly Aia Val Ser Val Pro Ile Tyr Ser Ser Ser Ser Leu 100 105 110 Ser Gin Ile Giu Trp Ile Ile Glu Asp Ser Gly Ala Val Leu Ala Ile 115 120 125 Thr Giu Thr Pro Asp His Thr Asp Leu Met Lys Asn Leu Val Ile Gly 130 135 140 Glu Asp Gly Thr Pro Ala Ile Lys Gly Ser Pro Ser Lys Leu Arg Arg 145 150 155 160 Ile Leu Glu Ilie Asn Ser Ser Ala Leu Giu Thr Leu Lys Phe Giu Gly 165 170 175 Arg Glu Leu Ser Asp Glu Leu Vai Trp Glu Arg Ile His Ala Thr Lys 180 185 190 Ala Ala Asp Leu Ala Ser Le'u Val Tyr Thr Ser Gly Thr Thr Gly Arg 195 200 205 WO 01/00805 PCT/IB00/00926 Pro Lys Gly Cys Glu Leu Ser His Tyr His Trp Leu Ala Glu Val Arg 210 215 220 Ala Leu Ile Thr Asn Asp Ile Gly Ala Ile Ala Met Pro Gly Ser Arg 225 230 235 240 Leu Leu Thr Phe Leu Pro Leu Ala His Val Leu Ala Arg Ala Val His 245 250 255 Leu Ala Phe Ala Val Thr Gly Ala Thr Gin Ser His Trp Ser Asp Phe 260 265 270 Ser Thr Leu Thr Leu Glu Leu Gln Arg Ser Arg -Pro Asn Leu Ile Leu 275 280 285 Gly Val Pro Arg Val Phe Glu Lys Val Arg Asn Ala Ala Ala Ala Asn 290 295 300 Ala Ala Asp Gly Gly Ala Ile Lys Arg Ile Met Phe Glu Arg Ala Glu 305 310 315 320 Lys Ala Ala Ile Glu Tyr Ser Met Ala Leu Asp Thr Ala Glu Gly Pro 325 330 335 Ser Lys Ser Gin Val Met Ala His Lys Ala Phe Asp Lys Leu Val Tyr 340 345 350 Ser Lys Ile Arg Ala Ala Val Gly Gly Asp Val Gin Tyr Ala Ile Thr 355 360 365 Gly Gly Ser Ala Met Gly Gin Glu Leu Leu His Phe Phe Arg Gly Val 370 375 380 Gly Met Thr Ile Tyr Glu Gly Tyr Gly Leu Thr Glu Ser Ala Ala Ala 385 390 395 400 Ala Ala Val Asp Phe Thr Asp Gin Lys Ile Gly Thr Val Gly Lys Pro 405 410 415 Met Gly Gly Met Thr Ile Lys Ile Asn Glu Asp Gly Glu Ile Met Leu 420 425 430 Lys Gly Glu Met Leu Phe Gin Gly Tyr Trp Asn Asn Pro Glu Ala Thr 435 440 445 Ala Glu Ala Leu His Asp Gly Trp Phe Asn Thr Gly Asp Leu Gly Glu 450 455 460 Leu Leu Glu Ser Gly His Leu Val Ile Thr Gly Arg Lys Lys Asp Leu 465 470 475 480 Ile Val Thr Ala Gly Gly Lys Asn Val Ser Pro Gly Pro Met Glu Asp 485 490 495 Ile Ile Arg Ala His Pro Leu Val Ser Gin Ala Met Val Val Gly Asp 500 505 510 Gly Lys Pro Phe Val Gly Leu Leu Val Thr Leu Asp Pro Asp Met Leu 515 520 525 WO 01100805 Lys Arg Trp Lys Leu Asn His Asi Ile Ala Glu 530 535 Glu Ile Ala Thr Asp Pro Ala Leu Arg Ala Glu 545 550 555 Asn Asn Ala Asn Ala Thr Val Ser His Ser Glu 565 570 Tyr Ile Leu Asp Arq Asp Leu Thr Giu Giu Ala 580 585 Thr Leu Lys Val Lys Arg Asn Val Val Val Arg 595 600 Ile Asp His Ile Tyr Asn Arg 610 615 <210> 61 <211> 1047 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1024) <223> RXA01060 <400> 61 ttccatttac acatctgttc gactcgcgcc cqaatgatgt Se r 540 Ile Ala Asp Arg Arg Thr Val Gin Asp Ala Ile Lys Arg 575 Glu Leu Thr 590 Tyr Ala Asp 605 PCTJIBOO/00926 Ser Val1 560 Ph e Pro Al a cgcatcaaca tgagaagcta cttccatact tttactgatt gataagaatg gacaaagaca atg aac cgc aca ctc cga Arg t gg Trp ttc Phe aa a Lys ga t Asp agg Arg tct gg C Gly act Thr gaa Glu ctt Leu gaa Glu tgg Trp aga t gg Trp ga t Asp t cc Se r cac His gca Ala gag Gi u att ctt Le u ccc Pro ctt Le u ct t Le u ctg Le u ct g Leu att gct Ala gac Asp tcc Ser g cg Al a agg Arg gaa Glu gag Arg ccg Pro gta Val atg Met cca Pro ttc Phe cgg Arg gcc Th r gag Glu aat As n ctg Le u gt g Val cac His acc Th r 100 ga t WO 01/00805 PCT/IBOO/00926 Ser Ala Val Arg Ile Ile Giu Ser Thr Lys Glu Ser Leu Ala Asp Gin 105 110 115 tac aga gat tac aaa tac gca ttc ttg cct gag cta att ttc cag gaa 499 Tyr Arg Asp Tyr Lys Tyr Ala Phe Leu Pro Giu Leu Ile Phe Gin Glu 120 125 130 tca cgc ggg atc ttt gac ttt gag ctg gag gga tac acc ctc aaa gta 547 Ser Arg Gly Ile Phe Asp Phe Giu Leu Glu Gly Tyr Thr Leu Lys Val 135 140 145 gga caa agc acg ctt tcc att cct tgg gac atg atc gcc aat ggt tat 595 Gly Gin Ser Thr Leu Ser Ile Pro Trp Asp Met Ile Ala Asn Gly Tyr 150 155 160 165 gtc cct gca agt ctt cgg aat ttt gga gaa tta atg gac cgt gac acc 643 Val Pro Ala Ser Leu Arg Asn Phe Gly Glu Leu Met Asp Arg Asp Thr 170 175 180 gga gat ctt gat gca gat ccc att ctg aga cct cga gaa ctc aaa ttt 691 Gly Asp Leu Asp Ala Asp Pro Ile Leu Arg Pro Arg Giu Leu Lys Phe 185 190 195 gag atc cat aat tgt cca gat ctt aat cct tgg ata atg cgg gaa act 739 Glu Ile His Asn Cys Pro Asp Leu Asn Pro Trp Ile Met Arg Giu Thr 200 205 210 ttt gat ttc atg atg gaa atc gca act gaa aca gga tgg ttc cat gct 787 Phe Asp Phe Met Met Glu Ile Ala Thr Glu Thr Gly Trp Phe His Ala 215 220 225 ctc aac ccc gca tat aat tcc gtc tac acc tac gat ctt att tct cgc 835 Leu Asn Pro Ala Tyr Asn Ser Val Tyr Thr Tyr Asp Leu Ile Ser Arg 230 235 240 245 atg cca gat ttc ctt gtt gaa ggg agc ttt cgt ccg cat tca gtg aaa 883 Met Pro Asp Phe Leu Val Giu Gly Ser Phe Arg Pro His Ser Val Lys 250 255 260 cqa tct tgg gaa aaa att caa aag ata gct aaa gct gtt gag tct tat 931 Arg Ser Trp Glu Lys Ile Gin Lys Ile Ala Lys Ala Val Glu Ser Tyr 265 270 275 gca tct cac gat tac tgc atg tca acg ctg aca cat gac tac agg gca 979 Ala Ser His Asp Tyr Cys Met Ser Thr Leu Thr His Asp Tyr Arg Ala 280 285 290 atc gag tta tcc ctg acg cct aca aaa act gag gaa cca agc aca 1024 Ile Giu Leu Ser Leu Thr Pro Thr Lys Thr Glu Glu Pro Ser Thr 295 300 305 taagaaaacc tcgcctgccc act 1047 <210> 62 <211> 308 <212> PRT <213> Corynebacterium giutamicum <400> 62 Met Asn Arg Thr Leu Arg Thr Leu Gly Trp Leu Ala Ala Val Ile Gin WO 01/00805 WO 0100805PCTIBOO/00926 Glu Asp Pro Tyr Ala Leu Asp Leu Phe Lys Ser Giu Ser Leu Leu Ile 130 Tyr Thr 145 Ilie Ala Met Asp Arg Glu Ile Met 210 Gly Trp 225 Asp Leu Pro His Ala Val His Asp 290 Glu Pro 305 <210> 63 Pro Val1 Met Pro Phe Arg Ala 115 Phe Leu As n Arg Leu 195 Arg Phe Ile Ser Giu 275 Tyr Glu As n Leu Val His Thr 100 Asp Gin L ys Gly Asp 180 Lys Glu His Ser Val1 260 Ser Arg Pro Trp Phe Thr Thr Asp Pro Asp Thr Asp Tyr Val Se r Le u Lys P he L ys Gin Giu Val1 Tyr 165 Th r Phe Thr Ala Arg 245 Lys Tyr Al a Phe Lys Asp 70 Arg Se r Tyr Se r Gi y 150 ValI Gly Glu Ph e Le u 230 Met Arg Ala Ile Ser Arg 55 Leu Giu Ala Arg Arq 135 Gln Pro Asp Ile Asp 215 As n Pro Ser Se r Glu 295 25 Giu Ser Leu His Glu Ala Trp Glu 90 Arq Ile.

105 Tyr Lys Ile Phe Thr Leu Ser Leu 170 Asp Ala 185 Asn Cys Met Met Ala Tyr Phe Leu 250 Glu Lys 265 Asp Tyr Ser Leu Le u Leu Le u Leu Ile T yr Asp Ser 155 Arg Asp Pro Glu As n 235 Val1 Ile Cys Thr Ser Ala Arg Glu Giu Ala Phe 140 Ile Asn Pro Asp Ile 220 Ser Glu Gin Met Pro Leu Met His Le u Ser Phe 125 Glu Pro Ph e Ile Le u 205 Al a Val Gly Lys Se r 285 Thr Val1 Giu Vai Ala Thr 110 Leu Le u T rp Gly Leu 190 Asn Th r Tyr Se r Ile 270 Thr Lys Pro Gin Le u Trp Lys Pro Glu Asp Glu 175 Arg Pro Glu Thr Phe 255 Al a Leu Thr Ser Thr WO 01/00805 <211> 1848 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1825) <223> RXN01722 <400> 63 ctcattcccc tcqctaaaag ctgcataaag tttcgacgtt PCTIBOO/00926 ttcaaagttg attgcttgca ctgtcgttgc gtqtcqcatg ccccqgctat tgttgattgc atg ctc agc acg atg Met Leu Ser Thr Met gt c Val1 gg t Gly gca Al a cat His t cg Ser gca Al a ga t Asp gct Al a 120 cca Pro gc9 Al a ctc Le u cgc cca Pro gat Asp cag Gln g cg Ala a tg Met t gc Cys cag Gln 105 gat Asp aaa L ys gcg Ala ctt Le u act atc Ile t gg T rp gtg Val ggt Gly gaa Glu aat Asn aat Asn caa Gin ttt Ph e ga Gly gtt Val1 175 tat ctc Le u ggc Gly gg g Gly att Ile cac His ccg Pro cac His ttg Le u att Ile atg Met 160 tac Tyr tcc 1 gag Glu ggt Gly gct Ala act Thr atg Met ct g Leu t ct Ser ggt Gly gga Gly 145 aag Lys aac Asn acc 163 21-1 259 307 355 403 451 499 547 595 643 691 WO 01/00805 WO 0100805PCTIBOOIOO926 Asp Glu Arg Thr Ala Ala Ala Ile C cg Pro ttg Le u ttC Ph e 230 atC Ile Ct t Le u gtg Val1 ct g Le u gg t Gi y 310 gg C Gly g9t Gly aat As n att Ile gag Glu 390 ga t Asp 9t t Val1 CCg Pro agc Ser 215 Ct 9 Leu 9 ca Al a t ct Ser gcg Ala aaa Lys 295 tct Se r gtg Val1 act Thr tat Tyr gtc Val1 375 att Ile gtg Val gaa Giu aag L ys 200 ttg Le u tg c Cys 9cg Ala 9 C9 Ala cat His 280 aat Asn 9 Cg Ala ga t Asp gtg Val1 cg a Ar g 360 aac As n cag Gin gaa Giu gag Glu 185 99 t Gly Cg C Arq tgt Cys ttt Phe C Cg Pro 265 g9C Gly

CCC

Pro gtg Val1 gtt Val1 tct Se r 345 gtC Val1 ga C Asp gtC Val1 aaa Lys gaa Glu gtg Val1 aCC Th r gtg Vai atq Met 250 aca Thr gtg Val cca Pro cct Pro gt C Val1 330 c gC Arg tCC Ser 9 C Gly C9gC Arg gaa Glu 410 aac Asn Cys 190 cac His ctc Le u cac His

CCC

Pro atc Ile 270 t9g Trp tct Ser atc Ile Gly ggt Gly 350 ttc Ph e gCg9 Ala g9tg Val g9CC Al a ttc Phe Tyr Ser Thr Gly Thr Ser Gly CgC Arg gca Ala gtc Val ctg Le u 255 att Ile att Ile ctg Leu acc Thr a tg Met 335 gtt Val1

CCC

Pro tcc Ser act Thr tca Ser 415 acc Thr tcg Ser gtg Val ctc Le u 240 9it 9 Val tcc Se r ca 9 Gin cg t Arq atg Met 320 a cg Thr tct Se r 9CC Ala acc Thr gca Al a 400 aca Th r 9CC Ala t cg Ser a cg Th r ccg Pro 245 ga t Asp Cg C Arg tac Tyr 99 C Gly tat Tyr 325 gig ValI tgg Trp

C

Arg 99 C Giy

CCC

Pro 405 gac Asp Cgc Arg 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 WO 01/00805 WO 0100805PCTI~IB00/00926 ggg Giy Cg C Arg 455 ca g Gin gtc Val1 acc Thr ctc Le u tat T yr 535 ga c Asp ga c Asp 440 gcc Ala C tg Leu att Ile atq Met cgc Arg 520 t gg T rp aag Lys gg a Gly ga t Asp aac As n ttc Phe 490 tac Tyr caa Gin ttt Phe gac Asp tct Se r gtc Val1 ctc Le u 475

CCC

Pro

CCC

Pro ctt Leu gtt ValI ct C Le u 555 gtc Vali atc Ile 460 atc Ile gat Asp ggc Gly Cg C Ar g gat Asp 540 Cg C Arq gat gga Asp Giy Giy Giy acg gaa.

Thr Giu 480 tgg gtg Trp Vai 495 cgc acc Arg Thr cta ccc Leu Pro gat aaa Asp Lys ctg cgc Leu Arg 560 acc Th r att Ile gtc Val1 ccc Pro acc Thr 515 atg Met gtc ValI gat atc Ile tac Tyr qaa Giu ctc Le u 500 qCC Aia ctg Leu ggt Giy ttc 1459 1507 1555 1603 1651 1699 1747 1795 1845 1848 Asn Giy Asp Phe Giu 565 gta att aag ctt aaa ggg cca ggt gaa Val Ile Lys Leu Lys Gly Pro Giy Giu 570 gct <210> 64 <211> 575 <212> PRT <213> Corynebacterium giutamicum <400> 64 Met Leu Ser Thr Met Gin Asp Vai Pro 1 5 Glu Tyr Giy Ser Thr Val His Gly Asp 25 Giy Aia Asp Gly Ile Glu Gin Aia Gin 40 Aia Arg Aia Ala Aia Leu Aia His Ala 55 Thr Gly Asp Gin Arg Val Aia Ser Met 70 taacttccct atttattccg Ser Leu Thr Arg Ile Leu Ile Thr Thr Trp Thr Phe Ser Ala Val His Asp Ser Leu Gly Tyr Asn Cys Ala Giu 75 Le u Gly Giy Ile His WO 01/00805 WO 0100805PCTf1B00100926 Met Leu Ser Gly Gly 145 Lys As n Thr Le u Glu 225 Se r Le u Th r Leu Glu 305 Trp Glu Gly Ser Phe Ala Val Ala Cys Met Gly Ala Val Phe Asn Pro 90 Le u Val Lys Phe Tyr 165 Gin Gi y Ser Th r Pro, 245 Asp Arg Tyr Gly Tyr 325 Val1 T rp Arg Met Val Glu Se r 150 Glu Asp Pro Leu Ph e 230 Ile Le u Val1 Leu Gly 310 Gly Giy Asn Ile Asn Ile Th r 135 Se r Al a Giu Pro Ser 215 Le u Al a Se r Ala Lys 295 S er ValI Thr Tyr Val 375 Gin 105 Asp Lys Al a Leu Thr 185 Gly Arg C ys Phe Pro 265 Gly Pro Val Val1 Se r 345 Val Asp Ile Pro Val Al a Asp 170 Ala Val Th r ValI Met 250 Thr ValI Pro Pro Val1 330 Arg Se r G ly Val1 A r c Arg His 155 Gly Al a Val Th r Pro 235 Se r Le u Pro Gi i Pro 315 His Pro Gin Gin Gi y 395 Phe Met Ala 140 Met Arg Ala T yr Asp 220 Ile Gly Ala Thr Arg 300 Ilie Val Pro Gly Val 380 Pro Ile Ala 125 Val1 Pro Ser Ile Ser 205* Se r Tyr Thr Lys Le u 285 Met ValI T rp Se r Arg 365 Met Trp Le u 110 Glu Vai Glu Th r Cys 190 His Leu His Pro Ile 270 Trp Se r Ile Gly Gi y 350 Phe Al a Val As n Gin Phe Gi y Val1 175 Tyr Arg Ala Val1 Le u 255 Ile Ile Le u Thr Met 335 Val1 Pro Ser Th r Asp Arg Asn Glu Gly Giu Ile Gin Val Arg 385 390 WO 01/00805 WO 0100805PCT1JIB00I00926 Pro Asp Val Glu LYS Glu Gly Gly Thr Ala Ser Thr 405 410 415 Asp Arg Gin Ser Cys 485 Ala GI u Pro Lys Val Thr Asp Ala 470 Ala Val1 Arg Giu T yr 550 Le u Thr 445 Arg Val1 Asp Ile Asp 525 Giu As n Thr Asp Gi y Thr Trp 495 Arg Leu Asp Leu Ala Gi y Gly Giu 480 Val Thr Pro Lys Ar g 560 Phe Giu Val Ile Lys Leu 565 Gly Pro Gly Giu Lys 575 <210> <211> 1848 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1825) <223> FRXA01722 <400> ctcattcccc tcgctaaaag ctgcataaag tttcgacgtt ctgtcqttgc gtgtcgcatg ccccggctat tgttgattgc cag gac gtc cca ctg tca tta acc Gin Asp Val Pro Leu Ser Leu Thr gtt cac ggt gat act ttg atc acc Val His Gly Asp Thr Leu Ile Thr gaa caa gca cag caa act ttt agt Giu Gin Ala Gin Gin Thr Phe Ser aga atc ctc Arg Ile Leu 15 acg tgg ggc Thr Trp Giy 30 gct gtg ggg Ala Val Giy ttcaaagttg attgcttgca atg ctc agc acg atg Met Leu Ser Thr Met 1 gag tac ggg tcc act Giu Tyr Gly Ser Thr ggt gcc gat ggc att Giy Ala Asp Giy Ile gct aga gct gcg gct Ala Arg Ala Ala Ala 115 163 211 259 ttg Leu g tg ValI 9 ca Ala atg Met gtt Val1 gaa Glu tct Ser 150 gaa Glu ga t Asp ccg Pro ttg Le u ttc Phe 230 atc Ile ctt Le u g tg ValI WO 01/00805 gct cat gcg Ala His Ala gcg tcg atg Ala Ser Met gtc gca tgc Val Ala Cys aat gat cag Asn Asp Gin 105 atc gct gat Ile Ala Asp 120 aca cca aaa Thr Pro Lys 135 agc gcg gcg Ser Ala Ala gcg ctc ctt Ala Leu Leu gaa cgc act Glu Arg Thr 185 ccg aag ggt Pro Lys Gly 200 agc ttg cgc Ser Leu Arg 215 ctg tgc tgt Leu Cys Cys gca gcg ttt Ala Ala Phe tct gcg ccg Ser Ala Pro 265 gcg cat ggc Ala His Gly 280 ctg Leu ctc Le u atg Met att Ile ccg Pro gtt Val1 gcc Al a gac Asp 170 gct Al a gtg Val acc Thr gtg Val1 atg Met 250 aca Th r gtg Val cat His tat Tyr 75 ggt Gly gtg Val cqc Arg cg t Arg cac His 155 ggc Gly gct Ala gtg Val1 a cg Th r ccg Pro 235 tcc Ser ttg Le u cct Pro 45 tct Se r tgc Cys gtg Val1 att Ile gct Ala 125 gtg Val1 c cg Pro t cc Ser at t Ile tcc Se r 205 tcc Se r tac Tyr act Thr aag Lys ctc Le u 285 PCTILBOO/00926 tta Leu gcg Ala ttt Phe ctc Le u 110 ga a Glu gtg Val gag Glu act Th r tgc Cy s 190 cac His ctc Le u cac His ccc Pro atc Ile 270 tgg Trp 99 t Gly gaa Gl u aat As n 95 aat Asn caa Gin ttt Phe gga Gly gtt Val 175 tat Tyr c gc Arg 9 ca Al a gtc Val1 ct g Le u 255 att Ile att Ile att Ile ca c His 80 ccg Pro cac His ttq Le u att Ile atg Met 160 tac Tyr tcc Ser t cg Ser gtg Val1 ctc Leu 240 gtg Val tcc Ser cag Gin act Thr atg Met ctg Le u tct Ser ggt Giy gqa Gly 145 aag Lys aac Asn a cc Th r ctt Leu gaa Glu 225 agc Se r ctt Le u acc Th r ctg Le u ggg Giy ga a Giu aac Asn ga a Giu gag Giu 130 ccg Pro ct g Leu t 99 Trp ggt Gly tat Tyr 210 cac His t gg Trp cct Pro a cg Thr atg Met 290 ga t Asp act Thr aag Lys gct Ala 115 atc Ile aat As n tat Tyr ccc Pro aca Th r 195 tta Le u ggc Gly 99 c Gly gga Gly ctg Le u 275 gt t Val ca 9 Gin a tg Met cag Gin 100 gaa Giu tta Le u ga t Asp tcc Ser gag Giu 180 t cg Ser cag Gln gaa G lu gt g Vali cccg Pro 260 cct Pro cac His cg a Arg ttt Phe t tg Le u gtg Val aaa Lys ttc Phe tat Tyr 165 ca g Gin gga Gi y t cg Ser acg Thr ccg Pro 245 gat Asp cgc Arg tac Tyr 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 wo 01/00805 WO 0100805PCTJJB00100926 ctg Leu gg t Gly 310 ggc Gly gg t Gly aat As n att Ile gag G iu 390 gat Asp gtt Val acc Thr gac Asp g ct Ala 470 gcc Ala gtc Val1 cgc Arg aaa Lys 295 tct Ser gtg Val1 act Thr tat Tyr gtc Val1 375 att Ilie gtg Val gaa Glu gg g Gly cgc Arq 455 ca g Gin gtc Val acc Th r ctc Le u ccc cca Pro Pro gtg cct Vai Pro gtt gtc Vai Vai 330 tct cgc Ser Arg 345 gtc tcc Val Ser gac qgc Asp Giy gtc cgc Vai Arg aaa gaa Lys Giu 410 gaa aac Giu Asn 425 gtt gga Vai Giy cgc .gat Arg Asp gaa aac Giu Asn ggc ttc Giy Phe 490 ctc tac Leu Tyr 505 gat caa Asp Gin gag Glu cca Pro 315 cac His cca Pro cag Gin cag Gin ggt Giy 395 ggt Giy gat Asp tct Ser gtc Val ctc Leu 475 ccc Pro ccc Pro ctt Leu cg t Arg 300 atc Ile g tg Vai cca Pro qgc Gly gtc Vali 380 ccg Pro gag Giu gtc Vai atc Ile 460 atc Ile ga t Asp Giy cg c Arg cg t Arg a tg Met 320 a cg Thr tct Ser g cc Ala acc Th r g ca Ala 400 a ca Thr gcc Aia gga Giy Gly ga a G iu 480 g tg Vali acc Thr ccc Pro gag Glu 305 tgg Trp ga a Giu gg t Giy tcc Ser ga c Asp 385 agt Ser t tc Phe ga c Asp ttc Phe gag Giu 465 gag Giu gaa Giu cgg Arg aac Asn cta Leu gag Glu acc Thr gaa Giu ctg Le u 370 cgc Arg tac T yr cg c Arg ggc Gly ctc Leu 450 t gg Trp gtt Val1 cg t Arg gaa Giu t gg Trp 530 tac Tyr ca g Gin t cc Se r agc Ser 355 cag Gin aac As n ttc Phe ga c Asp tgg T rp 435 acc Thr att Ile gtc Val ccc Pro acc Thr 515 a tg Met gtg ggc Vai Giy cgc tat Arg Tyr 325 acc gtg Thr Vai 340 cgg tgg Arg Trp tac cgc Tyr Arg gag ggc Giu Gly cac ccc His Pro 405 cat gac His Asp 420 ctg cgc Leu Arg atc caa le Gin tac tcc Tyr Ser gaa tgc Giu Cys 485 ctc gca Leu Ala 500 gcc gag Ala Glu ctg cca Leu Pro 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 WO 01/00805 PCT/IBOO/00926 gaa tat t99 aca ttt gtt gat gag gtg gat aaa acc tcc gtc ggt aaa 1747 Giu Tyr Trp Thr Phe Val Asp Glu Val Asp Lys Thr Ser Val Gly Lys 535 540 545 tat gac aag aag gac ctc cgc aac cac ctg cgc aac ggc gat ttc gaa 1795 Tyr Asp Lys Lys Asp Leu Arg Asn His Leu Arg Asn Gly Asp Phe Giu 550 555 560 565 gta att aag ctt aaa ggg cca ggt gar aaa taacttccct atttattccg 1845 Val Ile Lys Leu Lys Gly Pro Giy Xaa Lys 570 575 gct 1848 <210> 66 <211> 575 <212> PRT <213> Corynebacterium giutamicum <400> 66 Met Leu Ser Thr Met Gin Asp Val Pro 1 Giu Gly Ala Th r Met Le u Se r Giy Gly 145 Lys As n Th r Tyr Aia Arg Gly Glu Asn Glu Giu 130 Pro Le u Trp Gly 5 Ser Thr Vai His Gly Gly Ile Giu Gin Ala Ala Ala Leu Ala His Gin Arg Val Ala Ser Met Phe Ala Val Ala Gin Leu Met Asn Asp 100 Giu Val Val Ile Ala 120 Leu Lys Giu Thr Pro 135 Asp Phe Ser Ser Ala 150 Ser Tyr Giu Ala Leu 165 Giu Gin Asp Glu Arg 180 Ser Gly Pro Pro Lys 200 Ile Trp Val Gly Glu As n Asn Gin Ph e Gly Val 175 T yr Arg Ala 195 Leu Tyr Leu Gln Ser Leu Ser Leu Arg Thr WO 01/00805 WO 0100805PCT/IBOO/00926 Giu 225 Ser Le u Thr Leu Glu 305 Trp Gi u Gly Ser Asp 385 Se r Phe Asp Phe Giu 465 Giu G iu Arg Asn His Trp Pro Thr Met 290 Leu Giu Thr Gi u Le u 370 Arg T yr Arg Gly Leu 450 Trp Val1 Arg Glu Trp 530 Gly Gly Gly Leu 275 Val Tyr Gin Se r Ser 355 Gin Asn Phe Asp Trp 435 Thr Ile Val1 Pro Thr 515 Met Giu Val1 Pro 260 Pro His Val1 Arg Thr 340 Arg Tyr Giu His His 420 Le u Ile Tyr Glu Le u 500 Ala Le u Phe 230 Ile Le u Val1 Le u Gly 310 Gly Gly Asn Ile Giu 390 Asp Val1 Thr Asp Aia 470 Ala Val Arg Giu 215 Le u Ala Ser Ala Lys 295 Ser ValI T hr Tyr Val1 375 Ile ValI Giu Gi y Arg 455 Gin Val1 Thr Leu T yr 535 Cys Ala Ala His 280 As n Ala Asp Val1 Arg 360 As n Gin Giu Giu Asp 440 Ala Leu Ile Met Arg 520 T rp Cys Phe Pro 265 Gly Pro Val Val1 Se r 345 Val Asp Val Lys Giu 425 Val Arq Giu Gi y Le u 505 Asp Th r Val Met 250 Thr Val1 Pro Pro Val1 330 Arg Ser Gly Arg Giu 410 Asn Gi y Asp As n Phe 490 Tyr Gin Ph e Pro 235 Se r Le u Pro Giu Pro 315 His Pro Gin Gin Gly 395 Gly Asp Ser Val Le u 475 Pro Pro Le u ValI Tyr Thr Lys Leu 285 Met Val Trp Ser Arg 365 Met Trp Th r Le u Th r 445 Arg Val1 Asp Ile Asp 525 Glu His Pro Ile 270 Trp Ser Ile Gly Gly 350 Phe Ala Vai Ala Phe 430 Se r Ser Ala Lys Giu 510 Arg ValI Val1 Leu 255 Ile Ile Leu Thr Met 335 Val1 Pro Ser Thr Ser 415 Thr Asp Gly Xaa T rp 495 Arg Le u Asp Le u 240 Val Se r Gin Arg Met 320 Thr Se r Ala Th r Ala 400 Th r Ala Gly Gly Giu 480 Val1 Th r Pro Ly s WO 01/00805 PCTdTBOO/00926 Thr Ser Val Gly Lys Tyr Asp Lys Lys Asp Leu Arg Asn His Leu Arg 545 550 555 560 Asn Gly Asp Phe Giu Val Ile Lys Leu Lys Gly Pro Gly Xaa Lys 565 570 575 <210> 67 <211> 1401 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1378) <223> RXA01644 <400> 67 gtaggtagag gcttgtggtc actacttgtg gccacatttt aaaaaaatgc acaagaagag aaagcaaagc attatgagta acgccgtagc gcaggacctc atg acc atc gcc gac atc Ile ttc Phe gt t Val ttg Leu gag Glu tac Tyr tgg Trp atq Met tcc Se r gt c Va 1 ga t Asp gcc Ala ggt Gly cat His cgc Arg act Th r ga a Giu agg Arg 135 gag Giu gga Gly aac As n ttg Le u ccc Pro tgc Cys ctg Le u caa Gin 120 cac His ccc Pro gaa Giu tac Tyr atg Met atc Ile gca Ala tta Le u 110 aaa Lys aag Ly s atc Ile ga t Asp atc Ile gga Gi y ttt Phe tcc Ser aaa Lys gca Ala aaa L ys Met Thr Ile Ala 1 ttc cac atc act Phe His Ile Thr ccc tac cag ctg Pro Tyr Gin Leu aca gcg cca gga Thr Ala Pro Gly ctc atc gtg gaa Leu Ile Val Giu gcg ttg aag gag Ala Leu Lys Giu acc ttg cag atc Thr Leu Gin Ile 100 cag gaa att cca Gin Giu Ile Pro 115 atc aac ggg cta Ile Asn Gly Leu 130 att agc tac cac Ile Ser Tyr His 145 Asp g cc Ala ttt Phe.

ga t Asp ggt Giy ttc Phe atg Met cca Pro gca Ala tac Tyr 115 163 211 259 307 355 403 451 499 547 wo 01/0090s PCT/IBOO/00926 gac gtg ggc aat Asp 150 tat Tyr caa Gin Gly tac Tyr cag Gin 230 ga c Asp gg a Giy aac Asn ggc Giy cgc Arg 310 ctc Leu ga a Giu cac His ctc Le u tcg Val Giy Asn acc Thr ga a Giu ga t Asp gcc Aila 215 caa Gin ctc Leu ttc Phe aac Asn gga Giy 295 cac His act Thr gtg ValI gcg Ala gcc Aia 375 gaa tgc Cys aac Asn

C

Arg 200 gcc Ala tat Tyr gcg Ala ga t Asp tat Tyr 280 ctg Leu gca Aia ggc Giy ctg Leu t gg T rp 360 ggt Gly tg gcg Ala aaa Ly s 185 ctc Leu aaa Lys gag Giu gaa Giu g cg Ala 265 ccc Pro atg Met gg t Giy tct Ser cac His 345 tgc Cys gaa Giu gga gag Giu tat T yr 170 tac T yr cta Le u Ca c His tg T rp att Ile 250 atc Ile gac Asp Ct C Le u gca Ala ggc Gly 330 gaa Giu gaa Giu ccc Pro ttt 155 tat Tyr cgc Arg gac Asp ggt Gly gg t Gi y 235 cgc Ar g tca Ser tac Tyr aac Asn ttt Phe 315 acc Th r gaa Glu aac Asn act Thr gcc ttc tac tcc ctg ttt tta Phe Tyr Ser Leu Phe Leu cc a Pro ctc Le u gtg Val1 gtg Vai 220 caa Gin ttc Phe g ca Ala ttt Phe cac His 300 att Ilie ct g Leu aac As n ctc Le u gca Al a 380 cac a cg Thr atc Ile gga Giy 205 aaa Ly s gca Ala atg Met atc Ile ga a Glu 285 ag C Ser gat Asp atc Ile ctc Leu aaa Lys 365 cga Arg aac cca Pro ttt Phe 190 t gc C ys gcc Ala gag Glu ga t Asp ggc Giy 270 t tg Le u atc Ile cgc Ar g aag Lys cg c Arg 350 gaa Giu ctc Le u atc ga a Giu 175 gaa Giu gg t Giy atc Ile atc Ile tac Tyr 255 atc Ile ctc Leu acc Thr tac Tyr cac His 335 ttt Ph e aat Asn ttt Phe gtc t ca Ser aaa Lys tgg Trp gg a Giy aaa Lys 240 cgc Arg att Ile agc Se r tac Tyr att Ile 320 a tg Met ga t Asp t gg Trp gg C Giy cag ga t Asp ag t Se r ctg Le u gga Gly gt t ValI 225 cgc Arg ga t Asp ga a Giu a gc Se r cca Pro 305 ttc Phe cag Gin tac Tyr gag Giu ctg Le u 385 ctg gat tcc atg acc Asp Ser Met Thr ttg Le u cg t Arg gg c Giy 210 a cg Thr caa Gin gt t Vali cac His aaa Lys 290 gac Asp ccc Pro gac Asp caa Gin gaa Glu 370 tac Tyr cac gaa Giu ctg Le u 195 atg Met ct g Leu gg t Gly cca Pro at c Ile 275 ctc Le u aac As n ga c Asp aac As n cg C Arg 355 gca Ala a tg Met ca a gaa Giu 180 aaa Lys gtc Vai tct Ser ttg Leu ga a Giu 260 gg t Gly aaa Lys cgc Arg ggt Giy gg t Giy 340 acc Thr gtt Val gca Aila gt a gcc Ala ga a Glu cgc Arg ga a Giu ga a Giu 245 act Thr gtg Val1 aca Thr ccc Pro gaa Giu 325 ttc Phe ctg Le u gaa Giu gg t Giy ctg 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 WO 01/00805 Ser Giu Trp Gly 390 gqt gtg aaa ctc Gly Val Lys Leu atg tgg tgg act Met Trp Trp Thr 425 PCTJIBOO/00926 Phe Ala His Asn Ile Val Gin Leu His Gin Val Leu 395 400 405 gat gag cag gga agt cgc gga gaa gtt cct gaa aga Asp Glu Gin Giy Ser Arg Gly Giu Val Pro Glu Arg 410 415 420 atc taaagaaaca atgtttcttt tta Ile 1363 1401 <210> 68 <211> 426 <212> PRT <213> Corynebacterium giutamicum <400> 68 Met Thr Ilie Ala 1 Phe His Pro Tyr Thr Aia Leu Ile Aia Leu Thr Leu Gin Giu Ile Asn 130 Ile Ser 145 Asp Asp Ser Leu Leu Arg Gly Gly 210 Ile Gin Pro Vali Ly s Gin Ile 115 Gly Tyr Ser Giu Le u 195 Met Th r Le u Giy Giu Giu Ile 100 Pro Le u His Met Giu 180 Lys ValI Asp 5 Aia Phe Asp Giy Ph e Met Pro Al a Tyr Thr 165 Ala Glu Arg Ile Val Glu Ala Phe Val1 Leu Giu 70 Tyr Trp Met Ser Asp 150 Tyr Gin Gly Tyr Asp Gly Ala Asn 40 Gly Leu 55 His Pro Arg Gys Thr Leu Glu Gin 120 Arg His 135 Val Gly Thr Cys Glu Asn Asp Arg 200 Ala Ala 215 Ser 25 Thr Ala Gi y Phe Arg 105 Ala Ser Asn Ala Lys 185 Leu Lys Th r 10 Phe Asp Arq His Lys 90 Lys Pro Lys Giu Ty r 170 Tyr Leu His Thr Thr Al a Al a Pro 75 Arg Met Ala Ser The 155 Tyr Arg Asp Gly Gly Thr Ala Gly Pro Val Ser Tyr Leu Tyr Gly Pro Asp Asn Ala Trp Arg 125 Arg Asp 140 Tyr Ser Pro Thr Leu Ile Val Gly 205 Val Lys 220 Gin Ala Pro Glu Tyr Met Ile Ala Le u 110 Lys Lys Le u Pro Phe 190 Cys Ala Glu Ile Asp Ile Gi y Phe Se r Lys Ala Lys Phe Glu 175 Giu Gly Ile Ile Pro Ala Ala Asp Asp Thr Phe Le u Ala Le u 160 Se r Lys Trp Giy Lys Val Thr Leu Ser Giu Gin Gin Tyr Giu Trp WO 01/00805 225 Arg Asp Giu Ser Pro 305 Ph e Gin Tyr Gi u Leu 385 Leu PCT/IBOO/00926 240 Tyr Arg 255 Gin Val1 His Lys 290 Asp Pro Asp Gin Giu 370 Tyr His Gly Pro Ile 275 Le u Asn Asp As n Arg 355 Ala Met Gin Le u Glu 260 Gi y Lys Arg Gly Gly 340 Thr Val Ala Val1 Glu Asp Leu Ala Glu Ile Arg Phe Met Asp 245 Th r Val1 Th r Pro Glu 325 Phe Le u Glu G ly Le u 405 Gly Asn Gly Arg 310 Leu Glu His Leu Ser 390 Gly Ph e Asn Gly 295 His Thr Val1 Ala Ala 375 Glu Val Asp Tyr 280 Le u Ala Gly Le u Trp 360 Gi y Trp Lys 250 Ile Asp Leu Ala Gly 330 Glu Glu Pro Phe Asp 410 Se r Tyr As n Phe 315 Th r Glu Asn Thr Al a 395 Glu Ile Glu 285 Ser Asp Ile Le u Lys 365 Arg Asn Gly Gly 270 Le u Ile Arg Ly s Arg 350 Gi u Le u Ile Ser Ile Le u Thr T yr His 335 Ph e As n Phe Val1 Arg 415 Ile Ser Tyr Ile 320 Met Asp Trp Gi y Gln 400 Gi y Glu Vai Pro Glu Arg Met Trp Trp Thr Ile <210> 69 <211> 1437 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1414) <223> RXA02029 <400> 69 cgcacgtaca ctacttttag cagttaaccc gccgaacttt cttgattaag tattcattga gcgttcttgg cataaatccc actggaggtg catcaaacgt atg gca gag gca cgt Met Ala Glu Ala Arg 1 ttg cgc cat ctt gaa ccc att gat gtg gaa gag tgg cct. ggg gtg gcg Leu Arg His Leu Giu Pro Ile Asp Val Giu Giu Trp Pro Gly Val Ala 15 tca qtg ccc aac cta gcg ttt gct ggt gca cgc gca cgg caa gca gaa 115 163 211 Ser tat Tyr aat Asn g cc Ala tgg Trp ggg Gi y caa Gin ag t Se r gtt Val1 150 gg t Gly t cg Ser cga Arg cat His 9 Ct Ala 230 gtt ValI WO 01/00805 Val Pro As 2 cga ttt gc Arg Phe Al gac ccg ga Asp Pro As gca tcg gq Ala Ser G1 cgc agc ga Arg Ser GI ttt aaa cc Phe Lys Pr gCt gtg qa Ala Val As 120 tca ggt ga Ser Gly As 135 cca acc ac Pro Thr Th cgc aat ag Arg Asn Ar gag cct gt Glu Pro Va 18 gca gcg tc Ala Ala Se 200 gtg ttg ga Val Leu Gi 215 cga cgc ca Arg Arg G1 tca agc ct Ser Ser Le n a 'p y 9

U

C

0 5 t p t p t r 9 9 g 1 5

C

r g

U

g n 9

U

Leu aag Lys ctc Le u tgg T rp a gg Arg cg c Arg g cc Al a ggc Gly tta Le u gag Giu 170 9CC Al a ttt Phe ttt Phe gg a Gly gag Giu 250 Ala gcc Al a att Ile ct g Leu 75 ctt Leu 9g C Gly g ga Gly atg Met c gt Arg 155

CCC

Pro 9gtg Val ttg Leu

CCC

Pro act Th r 235 gag Glu Phe t gc Cys att Ile 60 gg g Gly gcc Ala aaa Lys ggC Gly agc Ser 140 acc Thr g ca Ala gag Giu ctt Leu agc Se r 220 gt t Val1 a cg Thr Ala agc Se r 45 gat Asp ctc Le u gac Asp cta Le u g ca Al a 125 gca Ala gca Ala t ct Se r cg t Arg gac Asp 205 agt Ser gac Asp ttt Phe Gly 30 aac As n cat His g ct Ala jt c Val1 tct Se r 110 cta Le u ttt Phe gtg Val1 cac His ga a Giu 190 ggc Gi y gg t Gly gc t Ala gtg Val1 Al a gca Ala gag Giu gag Giu ttg Le u 95 gga Gly

CCC

Pro ggt Gly aaa Lys ttt Phe 175 ga c Asp 9CC Ala gga Gly tta Leu Ctc Le u 255 att Ile Arg 99 t Gly gaa Glu agc Ser 80 acc Thr t cg Se r aat As n gga Gly.

agc Ser 160 gtg Val1 ctc Le u aaa Lys gct Ala a C9 Th r 240 gcc Ala

CCC

Pro Ala tta Le u tta Leu tac Tyr gct Ala ttc Phe ga a Giu gtt Val1 145 cat His ga t Asp ggc Gly gtt Val1 tta Le u 225 gct Ala gg t Gly t cg Se r Arg gtt Val ttC Ph e atg Met ttg Le u acc Thr ctC Le u 130 ttt Phe gtg Val atc Ile gag Giu aaa Lys 210 gct Ala gat Asp gtg Val1 C Ca Pro Gin ctg Le u t ca Ser gca Al a ttg Le u ctg Leu 115 att Ile gcc Ala ccg Pro acc Thr gct Al a 195 gcc Aia att Ile

CCC

Pro gag Giu C9 C Arg Ala cta Leu cg t Arg ggc Gly gg a Gly 100 cca Pro cgt Arg tCC Se r gga Gly aag Ly s 180 cag Gin gga Gly ttg Le u gcg Ala gag Giu 260 gaa Glu PCTIIBOO/00926 Giu gga 259 Gly ttg 307 Leu gag 355 Glu act 403 Thr ggg 451 Giy tta 499 Le u ggt 547 Gly gct 595 Ala 165 atc 643 Ile cgC 691 Arg agc 739 Ser gct 787 Ala caa 835 Gin 245 gat 883 Asp tgq 931 Trp att cac atC gag gtc att CCC cag gcg Ile His Ile Glu Val Ile Pro Gin Ala WO 01/00805 PTIO/02 PCT/IBOO/00926 ggt Gly 9gc Gly acc Th r 310 tgg Trp tgg Trp ga t Asp cat His ata Ile 390 t 99 Trp ctc 99C Gly aag Lys 295 acc Thr agt Ser cct Pro a 99 Arg tac Tyr 375 CgC Arg gt9 Val1 aat 9CC Ala 280 ca c His ggC Gly cc t Pro 9c9 Ala ga t Asp 360 cta Le u gaa Glu tat Tyr 9 ca 265 tac Tyr 99 t Gly 99C Gly gtg Val1 cC9 Leo 345 tct Ser aaa Lys 9 C 9 Al a cac His gtg gat tcg ata gtc gCC atg Asp Ser Ile t C Se r aac As n t Cjt Cys 330 cat His t ct Ser agc Ser 9C 9 Ala tac Tyr 410 caa aa g Lys g9ta Val1 315 t Ct Ser tac Tyr ctg Leu ValI 9j c a Ala 395 g C 9 Ala ttt CCJg A rg 300 9CC Ala g aa Glu cca Pro C9C Arg cag Gin 380 gat Asp ctt Leo 9gg Val Ala 285 ttt att Phe Ile atg caa Met Gin gcg att Ala Ile act 9tt Thr Val 350 gtq 9tg Val Val 365 ttg caa Leo Gln 99C ttt Gly Phe att gaa Ile Glu ttg acg Leu Thr 430 Met aaa Lys tcC Se r t Ct Se r 335 ga t Asp aag Lys C9 t Ar9 ga t Asp 9CC Ala 415 aca Thr gag Glu gCt Ala ttg Leu 320 ttg Leo gaa Giu gaa Giu gaa Glu 9CC Ala 400 ctg Leo aga Arg Ly s atc Ile 305 gta Val1 ctc Leo gtt Val1 aca Thr gtg ValI 385 9tc Val1 tta Leo aac As n aag cta gaa gtg gtg Leo 290 ga C Asp gct Al a aag Lys cat His cac Hi S 370 ttt Phe tac Tyr cgc Arg aga Arg Gio a ga Arg act Th r

C

Ala cag Gln 355 ttt Phe ga a Giu cgc Arg ctg Leo a 99 Arg 435 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1437 Leo Asn Aia Val Gin Phe Ala 425 cga Arg taagcaaaaa tcttttagac ctc <210> <211> 438 <212> PRT <213> Corynebacteriom glotamicom <400> Met Ala Glu Aia Arg Leo Ar9 His Leo Glu Pro Ile Asp Val Glu Glu 1 5 10 Trp Pro Gly Val Ala Ser Val Pro Asn Leo Ala Phe Aia Gly Ala Arg 25 Ala Arg Gln Ala Gbu Tyr Arg Phe Ala Lys Ala Cys Ser Asn Ala Gly 40 WO 01/00805 WO 0100805PCTA~BOO/00926 Leu Ser Ala Le u Leu 115 Ile Ala Pro Thr Ala 195 Ala Ilie Pro Glu Arg 275 Glu Arg Thr Ala Gin 355 Leu Gly Asn Asp Arg Leu Ala Ala Gly Glu Trp Arg Gly Thr Gly Phe 100 Pro Giy Gin Ala Arg Leu Ser Ser 135 Ser Gly Val Pro 150 Giy Ala Giy Arg 165 Lys Ilie Ser Giu 180 Gin Arg Arg Ala Gly Ser His Val 215 Leu Ala Ala Arg 230 Ala Gin Val Ser 245 Giu Asp Ile His 260 Giu Trp Giy Gly Val Vai Gly Lys 295.

Met Leu Thr Thr 310 Asp Gin Trp Ser 325 Tyr Ilie Trp Pro 340 Leu Val Asp Arg Pro Asp Leu Ile Ile Asp His Glu Glu Ser Ser Lys Val1 120 Gly Th r Asn Pro Ala 200 Le u Arq Ser Ile Ala 280 His G ly Pro Al a Asp 360 Leu Le u Gly Gi y Met A r c 155 Pro Val1 Le u Pro Tb r 235 Glu Ile Ser Lys ValI 315 Ser Tyr Le u Gly Ala Lys Giy Se r 140 Tb r Ala Glu Le u Ser 220 Val1 Thr Pro Ile Arg 300 Ala Glu Pro Arg Le u Asp Leu Ala 125 Aila Aia Ser Arg Asp 205 Ser Asp Ph e Gin Val1 285 Phe Met Ala Thr ValI 365 WO 01/00805 PCTIIBOO/00926 His Phe Ala Gly His Tyr Leu Lys Ser Val Gin Leu Gin Arg Giu 370 375 380 Phe Glu Gly Gin Ile Arg Glu Ala Ala Ala Asp Gly Phe Asp Ala 390 395 400 Tyr Arg Arg Met Trp Val Tyr His Tyr Ala Leu Ile Giu Ala Leu 405 410 415 Arg Leu Gly Cys Leu Asn Ala Val Gin Phe Ala Leu Thr Thr Arg .420 425 430 Arg Arg Gly Arq Arg 435 <210> 71 <211> 1140 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(1117) <223> RXA01801 <400> 71 tttggattcc tttccgatta tgtcttgatc gcccattctg tcacatccgg gttgtctaaa cacccgcgaa gatttcctgt. gatgtgccac actggttctc atg tct. aac Met Ser Asn gta gtg Val Val aac As n aaa Ly s gct Ala tac Tyr gtC Val ttc Phe ccg Pro gtc Val1 aat As n act Thr t cg Ser agc Se r gag Glu tct Ser 105 cag Gin tct Ser tgg T rp gag Giu gtg Val ttc Phe 9 tg Val1 gtg Va. 1 atc Ile gtg Val1 gg c Gly gcg Al a ttg Leu atg Met 1 gag Glu gat Asp ca g Gin gg t Gly gcc Al a gg a Gly gg t Gly WO 01/00805 WO 010080PCT[IBOO/00926 att tcc atg cat Ile t cg Ser tat Tyr 150 gtg Val1 g ca Ala aac As n ggC Gly ggg Gly 230 gat Asp ccg Pro gcg Al a tat Tyr gtt Val 310 gt t His gag Glu ca g Gin tgg Trp ttt Phe 185 ctt Le u gag Giu tct Se r ttt Phe gtg Val 265 ctg Leu cg c Arg ga c Asp tca 99a Gly a tg Met cg t Arg cg c Arg 170 gtt Val1 ga t Asp ctg Leu tgg T rp gt t ValI 250 gt g Val1 cg t Arg ca g Gln cg c Arg cat t cg Se r gcg Ala g ca Ala 155 atg Met gag Glu ggc Gly gcc Al a gcg Ala 235 gcc Ala gct Ala gaa Glu cct Pro aat As n 315 ttt Cgg Arg 125 ggc Gly t cg Se r cc t Pro g cg Ala ctg Le u 205 gtc Val1 atc Ile g tg ValI gtg ValI t tg Leu 285 ttt Phe gcc Ala a cc Th r a cg Thr ccc Pro atc Ile 160 atg Met ttc Ph e ttc Phe ca g Gln tcc Ser 240 ct g Le u a tg Met ga a Glu gg t Gly cca Pro 320 ca g Gin g cg Ala gg C Gly ttt Phe gct Al a gcg Ala 195 ca a Gin gag Glu tct Ser t cg Se r aac As n 275 at g Met gca Al a tac Tyr tca Se r ttc Phe ttt Phe t tg Le u ggc Gly 180 gt C Val cct Pro acc Th r cac His g ca Ala 260 agt Se r tct Ser gtg Val gaa G lu 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1117 1140 Val Asp Glu Ser His Phe Val 330 taaaaccgcc aaacttttga tag <210> 72 (211> 339 WO 01/00805 <212> PRT <213> Corynebacterium glutamicum PCT/IBOO/00926 <400> 72 Met Ser Asn Vai Val Asn Thr 1 Glu Asp Gin Gly Al a Gly Giy Giu Asp 145 Al a Le u Ile Ser Ile 225 Leu Met His Leu Le u Le u Gin Asp Giy Thr Giy Lys 130 Val1 Thr Trp Asp Th r 210 Gi u Arq Ala Gin Ala 290 As n Val1 ValI Val1 Asp Tyr Giy 115 Ala Giy Ph e Al a Ala 195 Gin Giu Ser Se r Asn 275 Met Phe Val Gin Leu Asn Ser Thr Thr Trp Ser Ser Giu Giu Ser Val Asp Glu Phe Ile Ser Val 105 Met His Gly 120 Pro Giu Met Gly Gin Arg Gly Trp Arg 170 His Phe Val -185 Ser Leu Asp 200 Ser Giu Leu Ser Ser Trp Glu Phe Val 250 Ser Val Val 265 Thr Leu Arg 280 Ile Arg Gln ValI Ile Val1 Gly Ala Le u Met Thr Pro* Ile 160 Met Phe Phe Gin Ser 240 Leu Met Glu Gly WO 01/00805 PCTJIBOO/00926 Val Arg Ala Val Leu Val Asp Lys Asp Arg Asn Ala Ala Phe Ser Pro 305 310 315 320.

Ala Asn Tyr Glu Asp Val Asp Glu Ser His Phe Val Thr Leu Phe Gin 325 330 335 Arg Ser Ser <210> 73 <211> 1086 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (101)..(1063) <223> RXN02512 <400> 73 gctggaaagt ccccattqgc taaggaattt accaaggcac cagcaggtgc gaaggcagat tacagcaaca ccaaataaaa attagccgag ggagcatcgc atg aag ccg aag gat Met Lys Pro Lys ttc Phe cta Leu c99 Arg atg Met gtt Val1 t g T rp ctt Leu tct Ser aac Asn ga a Glu cqt Arg ctt Le u cq c Arg ctg Le u ttc Pn e cg t Arg gg C Gly gc t Ala aat Asn ttt Phe 9qg Gly gct Ala gtg Val1 75 cgq Arg aag Lys aag Lys 9gc Gly 1 agc Se r ttg Le u aaa Lys gtc Val agc Se r cga Arg gat Asp CtC Le u cat His gca Ala cct Pro agc Se r ggt Gly tat Tyr gat Asp ttg Le u cca Pro 130 cat His ctg Leu att Ile gga Gly g cg Ala 9 Cg Ala cct Pro t tg Leu 115 cac His ggg Gly ggc Gly gct Ala aaa Lys ga a Giu C9g Arg gag Giu 100 ga t Asp agc Ser caa Gin Asp tat Tyr cgc Arg gqc Gly aac Asn tat T yr ctg Leu gaa Giu 9gc Gly ttC Phe 115 163 211 259 307 355 403 451 499 547 WO 01/00805 PTIOIO2 PCT[IBOO/00926 acc Th r 150 g tg Val1 Gly gg9t Gly gag Gi u ca g Gin 230 ttc Phe gat Asp aat Asn ttt Phe aat Asn 310 a gg Arg 155 agc Ser ttt Phe ctt Le u gg t Gly aca Th r 235 tgg Trp a cg Thr ccc Pro cc g Pro gtc Val ctt Le u gaa Glu ggg Gly gag Glu 220 ggt Gly ggt Gly gt g Val1 gct Ala gag Glu 300 gcg Ala acc Thr 180 tct Se r gg C Gly cct Pro cca Pro acc Th r 260 atg Met ctg Leu cag ttc Phe 165 gg t Gly gga Gly gtg Val g cg Ala tgg T rp 245 gtg Val1 gca Ala tgg T rp aca 595 643 691 '739 '787 835 883 931 979 1027 1073 1086 Arg Glu Gin Thr taggtgcgaa ccg gtg gca ita cag gag Pro Val Ala Leu Gin Glu gac aat Asp Asn 320 ttggaatggt ctg <210> 74 <211> 321 <212> PRT <213> Corynebacterium glutamicum <400> 74 Met Lys Pro Lys Asp Phe Cys Thr Ala Glu Asn Trp Ala Glu Asn Leu 1 5 10 Ser Ala Leu Gly Tyr Leu Ala Gly Trp Arg Phe Val Arg Met Leu Pro 25 WO 01/00805 PCT/IB00/00926 Leu Pro Ile Ala Arg Arg Val Phe Asp Leu Gly Ala Asp Leu Ala Ser 40 Lys Ser Gly Lys Gly Met Gly Gin Leu Arg Ala Asn Leu Ala Arg Val 55 Val Gly Ala Glu Asn Val Thr Gin Ala Leu Val Lys Gin Ala Thr Arg 70 75 Ser Tyr Ala Arg Tyr Trp Leu Glu Ala Phe Arg Leu Pro Ala Ile Ala 90 Arg Asp Pro Glu Leu Leu Ala Arg Leu Arg Lys Gly Thr Val Gly Leu 100 105 110 Asp Leu Leu Asp Glu Ser Leu Ala Ala Gly Lys Gly Val Val Leu Thr 115 120 125 Leu Pro His Ser Gly Asn Trp Asp Met Ala Gly Ala Phe Leu Ile Ser 130 135 140 His His Gly Gin Phe Thr Thr Val Ala Glu Arg Val Lys Pro Glu Arg 145 150 155 160 Leu Phe Glu Ala Phe Val Glu Phe Arg Glu Ser Leu Gly Phe Glu Val 165 170 175 Leu Pro Leu Thr Gly Gly Glu Arg Pro Pro Phe Glu Lys Leu Lys Glu 180 185 190 Arg Leu Thr Ser Gly Gly Ile Val Cys Leu Leu Gly Glu Arg Asp Leu 195 200 205 Arg His Ser Gly Val Glu Thr Thr Phe Phe Gly Glu Lys Thr Ser Met 210 215 220 Pro Ala Gly Pro Ala Gin Leu Ala Ile Glu Thr Gly Ala Ala Leu His 225 230 235 240 Val Val His Pro Trp Phe Asp Asp Asp Gly Trp Gly Leu Ser Val Ser 245 250 255 Asp Ala Val Thr Val Asp Asn Leu Ser Asp Thr Val Gin Arg Ile Ala 260 265 270 His Leu Phe Met Ala Asn Ile Thr Ala His Pro Ala Asp Trp His Met 275 280 285 Leu Gin Pro Leu Trp Phe Gly Asp Leu Asp Pro Glu Arg Leu Lys Arg 290 295 300 Ser Arg Glu Gin Thr Asn Val His Lys Pro Val Ala Leu Gin Glu Asp 305 310 315 320 Asn <210> <211> 1057 <212> DNA WO 01/00805 <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1057) <223> FRXA02512 <400> gctggaaagt ccccattggc taaggaattt accaaggcac PCTIBOOIOO926 cagcaggtgc gaaggcagat tacagcaaca ccaaataaaa attagccgag ggagcatcgc atq aag ccg aag gat Met Lys Pro Lys ttc Phe ct a Le u C9g Arg atg Met gt t Val tgg T rp ctt Leu tct Ser aac Asn acc Thr 150 gtg Val 99C Gly 9 cg Ala tg T rp gac Asp cta Le u g c9 Ala g cg Ala ttg Leu 105 9CC Ala atg Met g ca Al a cg a Arg ccg Pro 185 gaa Gl u cg t Arg ctt Le u c9 C Arg ctg Le u ttc Phe cgt Arq 99 C Gly gct Ala qa a Glu gaa Glu 170 ccg Pro 1 agc Se r ttg Le u aaa Lys gtc ValI aqc Se r cga Ar g gat Asp ctc Le u cat His 145 ttg Leu Ct 9 Le u CgC Arg gca Ala CCt Pro agc Se r 99t Gly tat T yr gat Asp ttg Le u cca Pro 130 cat His ttt Phe CCt Pro ctg Le u ctg Leu att Ile gga Gly gcg Ala 9C9 Ala cct Pro ttg Le u 115 ca c His 999 Gly ga a Glu ctc Le u aca T hr 195 Asp tat Tyr c9 C Arg 99 C G ly.

aac Asn tat Tyr ctg Le u ga a Gl u ggC Gly ttc Phe ttc Ph e 165 99 t Gly 99a Gly 115 163 211 259 307 355 403 451 499 547 595 643 691 WO 01/00805 WO 0100805PCT/IBOO/00926 ggt atc gtg tgc ctt ctt qqg gag cqt gac Gly Ile Val Cys Leu Leu diy Glu Arg 200 205 gag acc act ttt ttt ggt gag aag acc Giu Thr Thr Phe Phe Gly Glu Lys Thr 215 220 cag ctg gcc att gaa aca ggt qcg gcg Gin Leu Ala Ile Giu Thr Giy Ala Ala 230 235 ttc gat gac gac ggc tgg ggt ctc agc Phe Asp Asp Asp Gly Trp Gly Leu Ser 250 gat aat tta tcc gac acg gtg cag cgg Asp Asn Leu Ser Asp Thr Val Gin Arg 265 270 aat att acg gcg cac ccc gct gat tgg Asn Ilie Thr Ala His Pro Aia Asp Trp 280 285 ttt ggt gat ttg gat ccg gag cgt ctc Phe Giy Asp Leu Asp Pro Giu Arg Leu 295 300 aat gtt cac aaa ccg gtg gca tta cag Asn Val His Lys Pro Val Ala Leu Gin 310 315 <210> 76 <211> 319 <212> PRT <213> Corynebacterium giutamicum (400> 76 Met Lys Pro Lys Asp Phe Cys Thr Aia 1 5 Ser Ala Leu Giy Tyr Leu Ala Gly Trp 25 Leu Pro Ile Ala Arg Arg Vai Phe Asp 40 Lys Ser Gly Lys Giy Met Gly Gin Leu 55 Vai Gly Ala Giu Asn Vai Thr Gin Ala 70 Ser Tyr Ala Arg Tyr Trp Leu Giu Ala Arg Asp Pro Giu Leu Leu Ala Arg Leu 100 105 Asp ctg cgg cat tcc Leu Arg His Ser ggc gtg Giy Val 210 tcc Ser ctg Le u gta Val1 255 atc Ile cat His a ag Ly s gag Giu Giu Arg Le u Afg Le u Phe Ar g atg Met cac His 240 tcc Se r gca Ala atg Met cgc Arg Asn Phe Gly Ala Val Arg Lys cca Pro 225 gtg Val1 ga t Asp cat His ctc Leu tct Ser 305 Trp Val1 Ala Asn Lys Leu Gly gca Ala gtg Val g cc Ala ctt Le u caa Gin 290 a gg Arg Ala Arg Asp Leu Gin Pro Thr gga Giy cat His gtg Vai ttt Phe 275 ccc Pro gag Giu Glu Met Leu Ala Al a Al a Val 110 c ct Pro cca Pro a cc Thr 260 a tg Met ctg Leu cag Gin gcg Ala tgg Trp 245 gtg Val gca Ala tgg Trp aca Thr 787 835 883 931 979 1027 1057 WO 01/00805 PCTABOO/00926 Asp Leu Leu Asp Glu Ser Leu Ala Ala Gly Lys Gly Val Val Leu Thr 115 125 Leu His 145 Le u Le u Arg Arg Pro 225 Val1 Asp His Le u Se r 305 Pro 130 His Ph e Pro Le u His 210 Al a Val Al a Le u Gln 290 Arg His Gly Glu Le u Thr 195 Se r Gly His Val1 Phe 275 Pro Glu Gly Ph e Phe 165 Gly Gi y Val1 Al a Trp 245 Val1 Ala Trp Th r Asn Th r 150 Val Gly Gly Glu Gln 230 Phe Asp A~ n Phe Asn 310 Trp 135 Thr Glu G lu Ile Th r 215 Leu Asp Asn Ile GI y 295 ValI Asp ValI Phe Arg Val1 200 Thr Ala Asp Le u Thr 280 Asp His Ala Glu Glu 170 Pro Le u Ph e Glu Gly 250 Asp His Asp Pro <210> 77 <211> 1389 <212> DNA <213> Corynebacterium glutarnicum <220> <221> CDS <222> (101)..(1366) <223> RXA00899 <400> 77 tttgctgctc ccctacgtcq gactcccact gttcctgctt atgggatcgc catacatcaa ccggtgacgc caccgcatcc aacaagaaat caacgacctc atg gaa gac gtc cac 115 Met Glu Asp Val His 1 gac gac gtc ccc gac atc ccc acc gga atg gat gtc tcc gcg gaa gtt 163 Asp Asp Val Pro Asp Ile Pro Thr Gly Met Asp Val Ser Ala Glu Val 15 WO 01/00805 WO 0100805PCTIBOO/00926 gaa tet Glu Ser acc ggc Thr Gly cgg atg Arg Met ate tac Ile Tyr etc gaa Leu Giu cac gte His Val etc aac Leu Asn tgg cga Trp Arg 135 etc ate Leu Ile 150 gcc ccg Ala Pro aaa gac Lys Asp atg ate Met Ilie ate cge Ile Arg 215 gac tee Asp Ser 230 acc aca Thr Thr aaa gaa gte Val1 gga Giy ac Thr atc Ile cga Arg ggc Gly ege Arg 120 ege Arg ate Ile ag t Se r ete Le u tte Phe 200 ga c Asp gee Al a gaa G iu cg a ate aaa etc Ile Lys Leu aae aae qgc Asn Asn Gly gee gea ate Ala Ala Ile atg gee tgg Met Ala Trp 75 gee eac aae Ala His Asn age tgg aaa Ser Trp Lys 105 atg gge tte Met Gly Phe ege tte ege Arg Phe Arg gae gge eae Asp Gly His 155 tae eta eaa Tyr Leu Gin 170 atg gte gaa Met Val Giu 185 gee gge gac Ala Giy Asp eac gca gaa His Ala Giu ace aae etc Thr Asn Leu 235 eec aae etg Pro Asn Leu 250 etc ate ttg aae ege ege ete ace ege atg eca gca gtg Asn Arg Arg 30 Leu Thr Arg Met Pro Ala Val ttc Ph e ga c Asp ga t Asp ege Arg tae Tyr gee Al a ega Arg 140 aee Thr aag Lys etc Leu t gg Trp gee Ala 220 gtg Val1 eg e Arg tgc tee Ser gee Al a tac Tyr gte Val gg c Gly 110 tae Tyr ga e Asp tte Phe aae Asn ggC Gly 190 gte Vali gg e Gly etc Leu ttc Ph e ccc gac Asp gaa G iu ace Thr aaa L ys 95 tae T yr etc Le u etg Leu atg Met ate Ile 175 ec Pro ga a Giu tac Tyr ate Ile aae As n 255 t ac tac Ty r ga a Giu eaa Gin gte Val eac His a tg Met ege Arg gge Gi y 160 aaa Lys ate Ilie tee Ser ate Ile ccc Pro 240 tee Ser ttc ga a Giu ate Ile tte Ph e etc Le u etc Le u ccc Pro 130 eac His eaa Gin Gly tee Ser gaa Glu 210 aac Asn gge Gly gtt Val1 ec tee Ser tac Tyr tte Phe ett Le u aaa Lys 115 etc Le u eg e Arg aa t As n eg e Ar g tee Se r 195 gee Ala act Thr cet Pro eac His gac etc Le u gte Val gea Ala ttc Phe 100 aaa Lys caa Gin aaa Lys etc Leu ga a Giu 180 atg Met etc Leu ca a Gin gg t Gly ea c His 260 gaa 259 307 355 403 451 499 547 595 643 691 739 78-7 835 883 931 WO 01/00805 WO 0100805PCT/IBOO/00926 Lys Giu Arg Leu Ile Leu Cys Ser Pro Tyr Phe Ile Pro Asp Glu Ser 275 ctc Le u cta Le u tee Ser 310 ttc Ph e gac Asp etc Le u gc Ala cgc Arg 390 t qq 265 gee Ala tct Se r tac Tyr ccc Pro ac Th r 345 cgc Arg aac As n agt Se r eg c 270 gtc Val gaa Giu ca g Gin gac Asp 330 ggc Giy agc Se r ctc Le u ttc Ph e tac ace Th r caa Gin gca Aila 315 gte ValI aac Asn ttt Phe atc Ile a ag Lys 395 gtg t ca Ser gee Ala 300 etc Le u etc Le u gaa, Giu gg C Giy cac His 380 etc Le u gac Asp gcc tge Ala Cys 285 gac caa Asp Gin ctt gaa Leu Glu cac acc His Thr 9CC etC Ala Leu 350 etc aac Leu Asn 365 gaa etc Giu Leu ace ttg Thr Leu aat gtc Asn Val tac T yr ttc Phe gee Ala aag Lys 335 gga Gly tac Tyr aae As n ga t Asp atg Met cg c Arg gee Ala 9gc GI y 320 tac Tyr gte Val1 ga a Giu gee Ala aag Lys 400 eg t Arg gte Val gee Ala tac Tyr ga e Asp 340 tee Ser atg Met egt Arg ege Arg 9g Ala gaa Giu caa Gin caa Gin 325 ccc Pro aae As n ate Ile tac Tyr agt Ser 405 etg Le u 979 1027 1075 1123 1171 1219 1267 1315 1363 1389 Trp Arg Arg Arg Tyr Val 410 eag Gin tagtttggcg cgtttggagt geg <210> 78 <211> 422 <212> PRT <213> Corynebaeterium giutamicum <400> 78 Met Glu Asp Val His Asp Asp ValI Pro 1 5 Val Ser Ala Giu Vai Glu Ser Val Ile 25 Arg Met Pro Ala Val Thr Gly Gly Asn 40 Arg Giu Ser Leu Lys Arg Met Thr Ala 55 Ile Pro Thr Gly Met Asp Leu Asn Arg Arg Leu Thr Gly Phe Tyr Ser Asp Tyr Ile Asp Giu Ala Giu Giu wo 01/00805 WO 0100805PCTJIBOO/00926 T yr Pro Arg Arg Leu Asn 145 Se r Leu Val Asn Gly 225 Se r Ile Ile Gly Ile 305 Val Met Le u Ile Ph e Leu Leu Pro 130 His Gin Giy Ser Gi u 210 As n Giy Vali Pro Val1 290 Asp L ys Ile Giy Tyr Phe Le u Lys 115 Leu Arg Asn Arg Se r 195 Aia Thr Pro His Asp 275 Thr His Ile Aia Ser Vai Glu Ile 70 Aia Aia Leu Phe Asp His 100 Lys Giu Leu Gin Pro Trp Lys Met Leu 150 Leu Ile Ala 165 Giu Trp Lys 180 Met Giu Met Leu Asp Ile Gin Lys Asp 230 Gly Tyr Thr 245 His Aia Lys 260 Giu Ser Leu Val Glu Leu Aia Gin Ser 310 Tyr Gin Phe 325 Asp Pro Asp 340 Ser Asn Leu Tyr Ile Met Aia Glu Val1 Asn Arg 135 Ile Pro Asp Ile Arq 215 Ser Thr Giu Leu P he 295 Ser Pro Asp Asp Arg Gly Arg 120 Arg Ile Ser Le u Phe 200 Asp Al a Giu Arg Giu 280 Val1 Tyr Lys Thr Ile 360 Ala Ser 105 Met Arg Asp Tyr Met 185 Ala His Thr Pro Leu 265 Ala Ser Tyr Pro Thr 345 Arg Trp Asn Lys Phe Arg His 155 Gin Giu Asp Glu Le u 235 Le u Le u Thr Gin Ala 315 Vai Asn Phe Asp Ser Tyr Thr Gin Arg Tyr Ala Arq 140 Th r Lys Leu Trp Ala 220 Val1 Arg Cys Se r Ala 300 Le u Le u Giu Gly Gly Pro T rp 125 Pro Ala Lys Thr Tyr 205 His Gin Met Ser Ala 285 Asp Leu His Ala Le u 365 Val1 Gly 110 Tyr Asp Phe Asn Gly 190 Val Gly Le u Phe Pro 270 Cys Gin Giu Th r Leu 350 Asn Lys Tyr Le u Le u Met Ile 175 Pro Giu T yr Ile Asn 255 Tyr Tyr Ph e Ala Lys 335 Gly Tyr Val1 His Met Arg Giy 160 Lys Ile Ser Ile Pro 240 Ser Phe Arcj Ala Gly 320 Tyr Val1 Giu 355 Ile Ser 370 Leu Met Ile Ala Giy Asn Leu Ile Giu Leu Asn Ala WO 01/00805 PCT/iBOO0926 Leu Thr Asp Arg Tyr Arg Thr Val Ser Phe Lys Leu Thr Leu Asp Lys 385 390 395 400 Trp Asn Gin Arg Ser Trp Arg Arg Arg Tyr Val Asp Asn Vai Met Arg 405 410 415 Leu Thr Ser Ala Leu Gin 420 <210> 79 <211> 1452 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1429) <223> RXN00819 <400> 79 gatgaqgttc gtccgggaat cctcaaagac aatgcggtga aggtacttgg cctagccgct agcactgagc gcggatctca agcagaaaag gtcgtgcaac atg cgt gat ccc att Met Arg Asp Pro Ile caa Gin acc Thr gaa Giu ccc Pro cct Pro ttc Phe tac Tyr t cc Ser gtc Val cct Pro gca Ala tat Tyr cag Gin qca Ala 75 ctc Leu gg t Gi y cag Gin gca Ala gat Asp ctt Leu aat Asn etc Le u gag Gi u cg c Arg ttc Phe ttg Leu 125 acg Th r ttt ggt Phe Giy gaa acc Giu Thr gcc tgg Ala Trp gat ctg Asp Leu gtt cga Val Arg 80 gac atc Asp Ile acg gcc Thr Ala gcg cag Ala Gin cct gat Pro Asp ga a Giu gt g Val1 gca Ala ctt Leu ttc Phe.

gg t Gi y gtt Val 115 tct Se r ctc Le u gag Giu ttc Ph e ccg Pro gg t Gly tat Tyr gg c Gly gag Gi u cat ggc tct His Gly Ser gat ate Asp Ile WO 01/00805 WO 0100805PCT/IBOO/00926 gca Ala 150 tgg Trp ga t Asp tg0 Cys gat Asp aao Asn 230 ttc Phe t gg Trp tat Tyr t tg Leu ctg Le u 310 aaa Lys Cgg Arg att Ile tat Tyr 135 99 t Gly att Ile t tg Le u ttc Phe ogc Arg 215 aa t Asn aag Lys atg Met gtg ValI att Ile 295 gg c Gi y gag Glu gag Glu ttg Le u t ca Ser 375 99 t Gly atc Ile atc Ile ctc Le u 200 aaa Lys gto Val ga t Asp g cg Ala ogc Arg 280 cag Gin a tg Met gaa Glu acc Th r gac Aso 360 tat Tyr ct g Le u aat As n got Ala 185 gtg Val gcc Ala cgg Arg gtt Val1 gtc Val1 265 ago Se r ga a Glu atg Met aac Asn got Al a 345 aac As n ga a G lu 140 goa acc acg gcc act aag Ala ggt Gly 170 acc Thr gca Ala tca Ser gtg Val1 t cg Ser 250 g9t Gly a gg Arg aag Lys gtc Val too Ser 330 agt Ser ga t Asp ggc Gly Thr 155 gaa Glu t to Phe oct Pro ctg Leu tot Se r 235 ga a Gi u gog Al a ga a Glu oto Le u aaa Ly s 315 gog Al a t gg Trp gtt Val1 aco Thr Thr Ala Thr Lys aaa Lys gcc Al a ca 9 Gin 090 Arg 220 999 Gly t go Cys cag Gin oag Gin Ala 300 oto Le u otg Leu 909 Ala 900 Ala cac His 380 tgg T rp ga t Asp 190 gag Glu atg Met gog Ala 090 Arg ggt Gly 270 990 Gly a tg Met gat Asp aaa Lys gaa Giu 350 ttc Phe ato Ile gao gca Asp Ala 160 ggt 99t Gly Giy 900 gat Ala Asp gtg too Val Ser aat goa Asn Ala 225 otg cac Leu His 240 atg cgt Met Arg tat gaa Tyr Giu ccg ato Pro Ile 990 aat Gly Asn 305 oag oag Gin Gin 320 ttt aoo Phe Thr tgo gga Cys Gly 900 gat Ala Asp goa oto Ala Leu 385 gao Asp got Ala aac As n atg Met 210 cac His aao Asn too Ser gca Ala 909 Ala 290 oto Leu 909 Al a tog Ser 990 Gly 9 oa Ala 370 atc Ile aoo Th r too Ser cag Gin 195 gag Glu att Ile ato Ile ga t Asp 900 Al a 275 999 Gly aog Th r gga Gly otc Leu aac As n 355 gaa Giu gt t Val1 990 Gly act Thr 180 gtg Val1 att Ile aco Th r aat Asn gtg Val1 260 gtg Val ttc Phe 909 Al a att Ile aaa Lys 340 990 Gi y 900 Al a 99 n Gly gag Glu 165 got Ala aaa Ly s att Ile t at1 Tyr tot S er 245 909 Ala a ag Lys oag Gin tog Ser ttc Phe 325 ctt Leu ato Ile gto Val 09 f Arg 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 WO 01/00805 ncc att ctg q Xaa Ile Leu G1 390 gat ctt cat ga Asp Leu His As acg cac caa cc Thr His Gin Pr 42 ctg tca ccc ag Leu Ser Pro Ar 440 ctn Leu 395 ttc Ph e ccg Pro tgg T rp PCTIBOOIOO926 ttc ttt tta tat tat nac nct ttt gag gag 1315 Phe Phe Leu Tyr Tyr Xaa Xaa Phe Giu Glu.

400 405 cac cac ccc aaa cca tcg ttt ctt tcg aag 1363 His His Pro Lys Pro Ser Phe Leu Ser Lys 415 420 gcc agg acc tgg gct ttt cgc agt ggc gca 1411 Aia Arg Thr Trp Ala Phe Arg Ser Gly Aia 430 435 tgaacacctt ggcggacgca act 1452 <210> <211> 443 <212> PRT <213> Corynebacterium giutamicum <400> Met Arg Asp Pro Ile Gin Giy Aia Val 1 Ph e Arg Asp Gin Asp As n Cys Ile His 145 Aila Giy Asp Vali Le u Val1 Asp Th r Th r 100 Gly Giy Asp Gly Thr 180 Val Se r Val Ile Pro Gly Aia Le u Arg Le u 140 Thr Ly s Aia Gin Asp Le u Asn Leu Glu Arg Phe Le u 125 Thr Ala Arg Arg Ala WO 01/00805 195 Met Glu Ile 210 His Ile Thr Asn Ilie Asn Ser Asp Val 260 Ala Ala Val 275 Ala Gly Phe 290 Leu Thr Ala Ala Gly Ilie Ser Leu Lys 340 Gly Asn Gly 355 Ala Giu Ala 370 Ile Val Gly Xaa Phe Glu Phe Leu Ser 420 Arg Ser Gly 435 PCTIBOOIOO926 Se r Val Ser 250 Gly Arg Lys Val1 Se r 330 Ser Asp Gl y Xa a Tyr 410 Ser Arg Leu Se r 235 Glu Ala Glu Le u Ly s 315 Al a Trp ValI Th r Le u 395 Ph e Pro Trp Arg 220 Gly Cys Gin Gin Al a 300 Leu Le u Al a Ala His 380 Phe His Ala <210> 81 <211> 1030 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1007) <223> FRXA00819 <400> 81 tcttccgaac ggcctgcatg gtt ggt ggc tcc ccg gag cag gcq cag cqa ttg 53 Met Val Gly Gly Ser Pro Glu Gin Ala Gin Arg Leu WO 01/00805 WO 0100805PCT[IBOO/00926 ga t Asp gaa Glu act Thr tgg Trp ga t Asp gag Glu atg Met g cg Ala 125 cg c Arg gg t Gly gg C Gly a tg Met gat Asp 205 aaa L ys atc Ile cat His g ca Al a ggt Gly gat Asp tcc Se r gca Ala cac His cg t Arg ga a Giu 160 atc Ile aat Asn cag Gin a cc Thr aaa tct ggt gag gtc aag Lys Ser Gly Giu Val Lys ggc tct gat atc gca ggt Gly Ser Asp Ile Ala Gly gac acc ggc gag tgg att Asp Thr Gly Glu Trp Ile 50 gct tcc act gct gat ttg Ala Ser Thr Ala Asp Leu 70 aac cag gtg aaa tgc ttc Asn Gin Val Lys Cys Phe 85 atg gag att att gat cgc Met Gu Ilie Ile Asp Arg 100 cac att acc tat aac aat His Ile Thr Tyr Asn Asn 115 aac atc aat tct ttc aag Asn Ile Asn Ser Phe Lys 130 tcc gat gtg gcg tgg atg Ser Asp Val Ala Trp Met 145 150 gca gcc gtg aag tat gtg Ala Ala Val Lys Tyr Val 165 gcg ggg ttc cag ttg att Ala Gly Phe Gln Leu Ile 180 ctc acg gcg tcg ctg ggc Leu Thr Ala Ser Leu Gly 195 gcg gga att ttc aaa gag Ala Gly Ile Phe Lys Glu 210 tcg ctc aaa ctt cgg gag Ser Leu Lys Leu Arg Giu 225 230 ggc Gly gg t Gly atc Ile 55 atc Ile ctc Leu aaa Lys gt c Val ga t Asp 135 gcg Ala cgc Ar g cag Gin atg Met gaa Giu 215 acc Thr ga c Asp gtt ttt gca ctg acg Val1 ctg Le u aat As n gct Ala Val1 gcc Ala cg g Arg 120 gtt Val1 gtc Val a gc Ser gaa Giu atg Met 200 aac Asn gct Ala aac Asn Phe g ca Ala ggt Gly a cc Thr gca Ala tca Ser 105 gtg Val1 t cg Ser gg t Gly a gg Arg aag Lys 185 gt c Val1 tcc Ser agt Ser ga t Asp Al a acc Thr ga a Gi u ttc Phe cct Pro c tg Leu tct Ser gaa Giu gcg Al a gaa Glu 170 ctc Le u aaa Lys g cg Al a tgg T rp gtt Val1 250 Leu a cg Thr aaa Lys gcc Ala ca g Gin cg c Arg ggg Gly tgc Gys cag Gin 155 c ag Gin g cg Ala ctc Leu ctg Le u gcg Al a 235 gcc Al a Thr gcc Ala Cgg Arg a gg Arg gca Ala atc Ile gat Asp ctg Le u 140 gca Ala ttt Ph e ctc Leu acc Thr g cg Ala 220 Cgg Arg Cgg Arg 101 149 197 245 293 341 389 437 485 533 581 629 677 725 773 gaa atc tgc gga ggc Glu Ilie Cys Gly Gly 240 aac ggc atc att Asn Gly Ile Ile 245 WO 01/00805 ttc cat gcc gat gca gaa gcc gtc tat Phe His Ala Asp Ala Giu Ala Val Tyr 255 260 atc aat gca ctc atc gtt ggn cgn ncc Ile Asn Ala Leu Ile Val Gly Arg Xaa 270 275 tta tat tat nac nct ttt gag gag gat Leu Tyr Tyr Xaa Xaa Phe Giu Giu Asp 285 290 ccc aaa cca tcg ttt ctt tcg aag acg Pro Lys Pro Ser Phe Leu Ser Lys Thr 305 acc tgq gct ttt cgc agt gqc gca ctg Thr Trp Ala Phe Arg Ser Gly Ala Leu 320 325 tgaacacctt cgcggacgca act (210> 82 <211> 330 <212> PRT <213> Corynebacterium giutamicum <400> 82 Met Val Gly Gly Ser Pro Glu Gin Ala PCTIiBOO/00926 gaa 821 Glu ttt 869 Phe cac 917 His 300 agg 965 Arg 1007 1030 1 Ly s Gly Asp Al a Asn Met His As n Ser 145 Ala Gly Asp Gi y Thr ValI Ile Thr 115 Asn Val1 ValI Giu Ile Giu Ala Lys Ile 100 Tyr Ser Ala Lys 5 Val1 Ala Trp Asp Cys Asp As n Phe Trp Tyr Lys Gly Ile Leu 70 Phe Arg As n Lys Met 150 Val Phe 25 Ala Gi y Thr Ala Ser 105 Val Ser Gly Arg Gin 10 Ala Thr Glu Phe Pro 90 Le u Ser Giu Al a Glu Arg Le u Thr Lys Al a Gin Arq Gi y Cys Gin 155 Gin Leu Thr Ala Arq Arg Ala Ile Asp Le u 140 Ala Ph e Asp Giu Thr T rp Asp Giu Met Ala 125 Arg Gly Gly Ala Pro Lys Ile Thr Gly Gin 110 Arg Arg Ala Arg Gin Asp Asp Gly Ala Val Asn Le u Met Tyr Pro WO 01/00805 PTIO/02 PCT/IBOO/00926 Ala Gly Phe Leu Thr Ala 195 Ala Gly Ile 210 Ser Leu Lys 225 Gly Asn Gly Ala Giu Ala Ile Val Gly 275 Xaa Phe Glu 290 Phe Leu Ser 305 Arg Ser Gly <210> 83 <211> 391 <212> DNA Gin 180 Ser Phe Le u Ile Val1 260 Arg Glu Lys Al a 165 Leu Leu Lys Arg Ile 245 Tyr Xaa Asp Th r Le u 325 Ile Gly Giu Giu 230 Le u Ser Ile Leu His 310 Se r Gin Giu Met Met 200 Giu Asn 215 Thr Ala Asp Asn Tyr Glu Leu Gly 280 His Asp 295 Gin Pro Pro Arg Lys 185 Val1 Ser Se r Asp Gly 265 Xaa Tyr Ser Arg 170 Le u Lys Al a Trp Val1 250 Thr Le u Ph e Pro Trp 330 Ala Leu Leu Ala 235 Al a His Phe His Ala 315 Le u Thr Ala 220 Arg Arg Glu Phe His 300 Arg Met Asp 205 Ly s GI u Phe le Leu 285 Pro Thr Leu 190 Gin Met Ile His Asn 270 Tyr Lys Trp 175 Gly Asn Gln Gin Phe Thr Cys Gly 240 Ala Asp 255 Ala Leu Tyr Xaa Pro Ser Ala Phe 320 <213> Corynebacteriun glutarnicum <220> <221> CDS <222> (101)..(391) <223> FRXA01766 <400> 83 gatgaggttc gtccgggaat cctcaaagac aatqcqgtqa agcactgagc gcggatctca agcagaaaag gtcgtgcaac caa ggt Gin Gly acc gaa Thr Giu gaa gag Glu Glu qtt att cct tct gat Val Ile Pro Ser Asp gaa cqc gca qtt ctt Glu Arg Ala Val Leu aag cct tat att aat Lys Pro Tyr Ile Asn ctt Le u ctg Leu 30 gag Glu aggtacttgg cctagccgct atg cgt gat ccc att Met Arg Asp Pro Ile 1 ttc gca gaa gtt ctc Phe Ala Giu Val Leu cgc agg gtg ctt gag Arg Arg Val Leu Giu gat aag gca gtc ttc Asp Lys Ala Val Phe WO 01/00805 WO 0100805PCTIiBOOIO0926 ccc gat gag atc gtg cag ccc ctc caa gat ctg caa ttg ctt gat ccg 307 Pro Asp Giu Ile Val Gin Pro Leu Gin Asp Leu Gin Leu Leu Asp Pro 60 cct gca ctt cgg gaa gca ggg gag tcg gtt cga gac att ttc act ggt 355 Pro Ala Leu Arg Giu Ala Gly Glu Ser Val Arg Asp Ile Phe Thr Gly 75 80 ttc cgc aat ttt gaa ctc gcg cgc tgt gac atc aat 391 Phe Arg Asn Phe Glu Leu Ala Arg Cys Asp Ile Asn <210> 84 <211> 97 <212> PRT <213> Corynebacterium giutamicum <400> 84 Met Arg Asp Pro Ile Gin Gly Ala Val Ile Pro Ser Asp Leu Phe Gly 1 5 10 Phe Ala Glu Val Leu Thr Glu Ala Giu Arg Ala Val Leu Leu Giu Thr 25 Arg Arg Vai Leu Glu Giu Glu Val Lys Pro Tyr Ile Asn Glu Ala Trp 40 Asp Lys Ala Val Phe Pro Asp Glu Ile Val Gin Pro Leu Gin Asp Leu 55 Gin Leu Leu Asp Pro Pro Ala Leu Arg Glu Ala Gly Giu Ser Val Arg 70 75 Asp Ile Phe Thr Gly Phe Arg Asn Phe Glu Leu Ala Arg Cys Asp Ile 90 Asn <210> <211> 1659 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (101)..(1636) <223> RXN01762 <400> tggagtgatg aattttccta tagaacgttt tttaaacgat tgacttttta aacgtttacg cttttaatga cttcaaacgt gatctaaagc acaaaggaga atg aaa gtg aac ctc 115 Met Lys Val Asn Leu 1 gga ata gga agc tac cca cga cgc agg gca act gtt cga cca gag tcc 163 Gly Ilie Gly Ser Tyr Pro Arg Arg Arg Ala Thr Val Arg Pro Glu Ser WO 01/00805 PCTL1BOO/00926 15 act gca atc gaa ttc gaa qgc acc agc atc acc tac gga gaa ttc agc 211 Thr Ala Ile Giu Phe Giu Gly Thr Ser Ile Thr Tyr Giy Giu Phe Ser 30 aaa cga gtc aat cgg ctt gga cat gct ctt cta gac ctc ggc gtt gcg 259 Lys Arg Vai Asn Arg Leu Gly His Ala Leu Leu Asp Leu Gly Val Ala 45 cac caa gat cga gta gct tat gtc gga ttc aac cac cct gcc ctg cta 307 His Gin Asp Arg Val Ala Tyr Val Gly Phe Asn His Pro Ala Leu Leu 60 gaa gtg ttc ttt tca acg aac ctc att ggg gca aca ccc gtg ctt gtt 355 Glu Val Phe Phe Ser Thr Asn Leu Ile Giy Ala Thr Pro Val Leu Val 75 80 aac cct cgc cta tcg gca aac gaa atc gat tac atc atc caa gac agc 403 Asn Pro Arg Leu Ser Ala Asn Giu Ile Asp Tyr Ile Ile Gin Asp Ser 95 100 ggt gcg agc atc gtg ttt tac gga atc gac ctc atc gag cac gcc act 451 Giy Ala Ser Ile Val Phe Tyr Gly Ile Asp Leu Ile Giu His Ala Thr 105 110 115 tac ctc caa gaa ctc cat cca gag atc atc atg gtg gcc gtt gaa ggc 499 Tyr Leu Gin Glu Leu His Pro Glu Ile Ile Met Val Ala Val Giu Gly 120 125 130 gat gag ggt cca ggt ttg cgt cga aaa gcg ctt att gaa gcg gcg agc 547 Asp Glu Gly Pro Giy Leu Arg Arg Lys Ala Leu Ile Glu Ala Ala Ser 135 140 145 gac gcc gac atc gac cta gaa gtc agc gat gat gac ctg gtg ctg ctc 595 Asp Ala Asp Ile Asp Leu Giu Val Ser Asp Asp Asp Leu Val Leu Leu 150 155 160 165 atg tac acc tcc gga acc act ggc cgc cca aag ggc gcc atg tta tcc 643 Met Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys Gly Ala Met Leu Ser 170 175 180 cac cga aac ctc ttc ttt aac tac ttc aat gcc ctg ctc agc cag gaa 691 His Arg Asn Leu Phe Phe Asn Tyr Phe Asn Ala Leu Leu Ser G* n Giu 185 190 195 att gaa caa ggc gcg gta ctt tta tcc act gcg ccg tta ttc cac atc 739 Ile Glu Gin Gly Ala Val Leu Leu Ser Thr Ala Pro Leu Phe His Ile 200 205 210 gcg ggc ctc aac atg acc acc atc ccg gtg atg atg aag ggc gga aag '787 Ala Gly Leu Asn Met Thr Thr Ile Pro Val Met Met Lys Gly Gly Lys 215 220 225 gtg atc atc cac cgc gaa ttC cgg gca gag cac gtc ctc gac gaa atc 835 Val Ile Ile His Arg Glu Phe Arg Ala Glu His Val Leu Asp Giu Ile 230 235 240 245 gaa cgc tcc aag gta tcc gaa tcc ttc atg gtg cca gcg atg atc gac 883 Giu Arg Ser Lys Val Ser Giu Ser Phe Met Val Pro Ala Met Ile Asp 250 255 .260 WO 01/00805 PTIO/02 PCT/IBOO/00926 atg Met cgc Arg atc Ile ga a Giu 310 cac His gt g ValI gt t Val1 gaa Glu atc Ile 390 aaa Lys gaa Glu gga Gly atc Ile gaa Glu 470 cac His ttg Le u gcc Ala t gg Trp 295 acc Thr ctt Le u ga c Asp ctc Le u ga c Asp 375 gcc Ala ga c Asp caa Gin gtc Val1 cga Arg 455 cta Leu atc Ile tcc Se r atc Ile 280 caa Gin gca Ala gga Gly ccg Pro atc Ile 360 acc Th r atc Ile a tg Met gca Al a ccc Pro 440 ga a Gi u cta Le u atc Ile aac Asn 265 atg Met gga Gly ccg Pro acc Thr aaa Lys 345 cg c Arg gcc Al a aaa Lys t ac Tyr Ct C Le u 425 ga t Asp agc Se r 9gc Gly ga a Glu cca Pro gg C Gly gac Asp gcc Al a 315 gg t Gly ggc Gi y cca Pro gca Ala gaa Glu 395 t cc Ser gaa Glu cga Ar g ct g Le u gtc Val1 475 ctc Leu tca Ser ggc Gly gta Val1 300 tgt Cys cg a Arg gaa Glu cat His ct a Leu 380 gac Asp 9gc Gly ct g Leu tgg Trp acc Thr 460 cta Leu ccc Pro ttt Phe tct Ser 285 aaa Lys atc Ile g cc Ala gaa Glu gtg Val1 365 caa Gin ggc Gly ggC Gly gaa Glu ggc Gly 445 aac Asn g cc Ala cgc Arg gcc Ala 270 ccc Pro att Ile ctc Leu cac His gtc ValI 350 atg Met aat As n tac Tyr qaa Glu gca Ala 430 gaa Glu cca Pro aga Arg aac Asn gaa Giu ctt Le u gtc ValI gag Glu ttc Phe 335 ccc Pro acc Th r ggc Gl y tac Tyr aac As n 415 gtc Val act Thr cca Pro tac Tyr gcc Al a 495 cgc Arg agc Se r caa Gin g ca Ala 320 ttc Phe a cc Th r gga GI y t g T rp acc Th r 400 att Ile ctt Le u gg c Gly acc Thr aaa Lys 480 aca Thr ga c Asp ga a G lu ggc Gly 305 aca Thr acc Thr gga Gly tac Tyr tac Tyr 385 atc Ile tac Tyr ga t Asp atc Ile gga Gly 465 ctt Leu ggC Gly ctt Le u cg t Arg 290 ttc Ph e ga c Asp ga c Asp gag Glu t gg T rp 370 cac His aaa Lys ccc Pro gcc Ala gcc Ala 450 ccg Pro cca Pro aaa Lys t ct Ser 275 gcg Ala ggc Gly aca Thr atc Ile gcc Ala 355 aac As n t cc Ser gac Asp gca Ala gca Ala 435 ttt Phe ga a Glu cg a Arg atc Ile t cc Se r ct g Leu atg Met agc Ser aaa Lys 340 ggc Gly cga Arg 9ga Gly cgc Arg gaa Glu 420 gt c Val1 gtc Val cta Le u gaa Glu caa Gin 500 ctt Leu cga Arg aca Thr a ca Thr 325 cta Leu gaa Gi u cca Pro gat Asp atc Ile 405 gtc Val1 atc Ile tcc Se r cga Arg atc Ile 485 aag Lys 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 WO 01/00805 PCTIBOOIOO926 aac atc ctg cga gac ttc acc atc ccc gtt tca taaacccccc aacgtcactt 1656 Asn Ile Leu Arg Asp Phe Thr Ile Pro Val Ser 505 510 tga 1659 <210> 86 <211> 512 <212> PRT <213> Corynebacteriun glutamicum <400> 86 Met Lys Val Asn Leu Gly Ile Gly Ser Tyr Pro Arg Arg Arg Ala Thr 1 5 10 Vai Arg Pro Glu Ser Thr Ala Ile Giu Phe Giu Gly Thr Ser Ile Thr 25 Tyr Gly Giu Phe Ser Lys Arg Val Asn Arcj Leu Gly His Ala Leu Leu 40 Asp Leu Gly Val Ala His Gin Asp Arg Val Ala Tyr Val Gly Phe Asn 55 His Pro Ala Leu Leu Giu Val Phe Phe Ser Thr Asn Leu Ile Gly Ala 70 75 Thr Pro Val Leu Val Asn Pro Arg Leu Ser Ala Asn Giu Ile Asp Tyr 90 Ile Ile Gin Asp Ser Gly Ala Ser Ile Val Phe Tyr Gly Ile Asp Leu 100 105 110 Ile Glu His Ala Thr Tyr Leu Gin Giu Leu His Pro Gu Ile Ile Met 115 120 125 Val Ala Val Glu Gly Asp Giu Gly Pro Gly Leu Arg Arg Lys Ala Leu 130 135 140 Ile Giu Ala Ala Ser Asp Ala Asp Ile Asp Leu Glu Val Ser Asp Asp 145 150 155 160 Asp Leu Val Leu Leu Met Tyr Thr Ser Gly Thr Thr Gly Arg Pro Lys 165 170 175 Gly Ala Met Leu Ser His Arg Asn Leu Phe Phe Asn Tyr Phe Asn Ala 180 185 190 Leu Leu Ser Gin Giu Ile Giu Gin Giy Ala Val Leu Leu*Ser Thr Ala 195 200 205 Pro Leu Phe His Ile Ala Gly Leu Asn Met Thr Thr Ile Pro Val Met 210 215 220 Met Lys Gly Gly Lys Val Ile Ile His Arg Giu Phe Arg Ala Giu His 225 230 235 240 Val Leu Asp Giu Ile Giu Arg Ser Lys Vai Ser Giu Ser Phe Met Vai 245 250 255 WO 01/00805 PCT/IB00/00926 Pro Ala Met Ile Asp Met Leu Ser Asn His Pro Ser Phe Ala Glu Arg 260 265 270 Asp Leu Ser Ser Leu Arg Ala Ile Met Val Gly Gly Ser Pro Leu Ser 275 280 285 Glu Arg Ala Leu Arg Ile Trp Gin Gly Arg Asp Val Lys Ile Val Gin 290 295 300 Gly Phe Gly Met Thr Glu Thr Ala Pro Gly Ala Cys Ile Leu Glu Ala 305 310 315 320 Thr Asp Thr Ser Thr His Leu Gly Thr Ala Gly Arg Ala His Phe Phe 325 330 335 Thr Asp Ile Lys Leu Val Asp Pro Lys Thr Gly Glu Glu Val Pro Thr 340 345 350 Gly Glu Ala Gly Glu Val Leu Ile Arg Gly Pro His Val Met Thr Gly 355 360 365 Tyr Trp Asn Arg Pro Glu Asp Thr Ala Ser Ala Leu Gin Asn Gly Trp 370 375 380 Tyr His Ser Gly Asp Ile Ala Ile Lys Asp Glu Asp Gly Tyr Tyr Thr 385 390 395 400 Ile Lys Asp Arg Ile Lys Asp Met Tyr Ile Ser Gly Gly Glu Asn Ile 405 410 415 Tyr Pro Ala Glu Val Glu Gin Ala Leu Gin Glu Leu Glu Ala Val Leu 420 425 430 Asp Ala Ala Val Ile Gly Val Pro Asp Glu Arg Trp Gly Glu Thr Gly 435 440 445 Ile Ala Phe Val Ser Ile Arg Glu Ser Tyr Leu Thr Asn Pro Pro Thr 450 455 460 Gly Pro Glu Leu Arg Glu Leu Leu Gly Ser Val Leu Ala Arg Tyr Lys 465 470 475 480 Leu Pro Arg Glu Ile His Ile Ile Glu Glu Leu Pro Arg Asn Ala Thr 485 490 495 Gly Lys Ile Gin Lys Asn Ile Leu Arg Asp Phe Thr Ile Pro Val Ser 500 505 .510 <210> 87 <211> 1372 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1363) <223> FRXA01762 WO 01/00805 <400> 87 tggagtgatg aattttccta tagaacgttt tttaaacgat cttttaatga cttcaaacgt gatctaaagc acaaaggaga PCTIIBO0/00926 tgacttttta aacgtttacg gga Gly act Thr aaa Lys cac His gaa Glu aac Asn gg t Gly tac Tyr ga t Asp ga c Asp 150 a tg Met cac His att Ile g cg ata Ile gca Al a cga Arg caa Gin gtg ValI cc t Pro gcg Ala ctc Le u gag Gi u 135 gcc Al a tac Tyr cg a Arg gaa Giu ggC gga Gi y atc Ile gtc Val1 ga t Asp ttc Phe cg c Arg aqc Se r ca a Gin 120 gg t Gi y ga c Asp acc Thr aac Asn caa Gin 200 ctc agc Se r ga a Gi u aat As n cga Arg ttt Phe cta Leu atc Ile 105 ga a Glu cca Pro atc Ile tcc Se r ct C Le u 185 9gc Gly aac cca Pro gaa Glu ctt Le u gct Ala a cg Thr 75 gca Al a ttt Phe cat His ttg Le u cta Leu 155 acc Thr ttt Phe gta Val1 acc cga Arg gg c Gly gg a Gly tat Tyr 60 aac Asn aac Asn tac Tyr cca Pro cg t Arg 140 ga a Giu act Th r aac As n ctt Le u acc cgc Arg acc Thr cat His 45 gt c Val ctc Le u ga a Glu gga Gly gag Glu 125 cga Arg gtc Val1 Gly tac Tyr tta Leu 205 atc agg Arg agc Ser gct Ala gga Gly att Ile atc Ile atc Ile 110 atc Ile aaa Lys agc Se r cg C Arg ttc Phe 190 t cc Se r ccg g ca Ala atc Ile ctt Le u ttc Phe ggg Gly ga t Asp ga c Asp atc Ile g cg Ala ga t Asp cca Pro 175 aat Asn act Thr gt g act Thr acc T hr cta Le u aac As n gca Ala 80 tac Tyr ctc Le u atg Met ctt Le u ga t Asp 160 aag Lys gcc Al a gcg Ala a tg Met 1 gtt Val1 tac Tyr ga c Asp ca c His aca Thr atc Ile atc Ile gtg Val1 att Ile 145 gac Asp ggc Gly ctg Le u ccg Pro Lys cga Arg gga Gly ctc Le u cc t Pro ccc Pro atc Ile gag Glu gcc Al a 130 gaa Glu ctg Leu gcc Al a ctc Le u tta Leu 210 Asn gag Giu ttc Phe gt t ValI ctg Le u ctt Leu gac Asp 100 gcc Ala gaa Glu g cg Ala ctg Leu tta Le u 180 cag Gin cac His aaa gtg aac ctc Leu tcc Ser agc Se r gcg Al a ct a Le u gtt Val1 agc Se r act Thr ggc Gly ag c Ser ctc Le u 165 t cc Ser ga a Giu atc Ile 115 163 211 259 307 355 403 451 499 547 595 643 691 739 787 atg atg aag ggc gga aag wo 01/00805 WO 0100805PCTIIBOO/00926 Ala Giy Leu 215 gtg atc atc Vai Ile Ile 230 gaa cgc tcc Giu Arg Ser atg ttg tcc Met Leu Ser cgc gcc atc Arg Ala Ile 280 atc tgg caa Ilie Trp Gin 295 gaa acc gca Giu Thr Ala 310 cac ctt gga His Leu Gly gtg gac ccg Val Asp Pro gtt ctc atc Vai Leu Ile 360 gaa gac acc Glu Asp Thr 375 atc gcc atc Ile Ala Ile 390 aaa gac atg Lys Asp Met taagtaccc Asn Met Thr Thr Ile Pro Vai Met Met Lys Giy Giy Lys 225 220 Cac cgc His Arg aag gta Lys Val 250 aac cac Asn His 265 atg gtg Met Val gga cgc Gly Arg ccg ggc Pro Giy acc gca Thr Ala 330 aaa acc Lys Thr 345 cgc gga Arg Gly gcc agc Ala Ser aaa gat Lys Asp tac atc Tyr Ile 410 gaa Giu 235 tcc Ser c ca Pro ggc Gly ga c Asp 9CC Ala 315 ggt Gi y gg C Giy cca Pro gca Al a ga a Gi u 395 tc Se r ttc Phe gaa Glu tca Ser gg C Gly gta ValI 300 tgt Cys cg a Arg ga a Giu cat His cta Le u 380 gac Asp ggc Gly Cgg Arg t cc Ser ttt Phe tct Se r 285 aaa Lys atc Ile gcc Ala gaa Gi u gtg ValI 365 caa Gin Gly ggc Giy gica Ala ttc Phe gcc Al a 270 ccc Pro att Ile ctC Leu cac His gtc Val1 350 atg Met aat Asn tac Tyr gaa Glu gag Glu atg Met 255 ga a Giu ctt Leu gtc Val1 gag Giu ttc Phe 335 ccc Pro acc Thr ggc Gly tac Tyr aac Asn 415 cac His 240 gtg Val cg C Arg agc Se r caa Gin gca Al a 320 ttc Phe acc Thr gga Giy t 99 Trp acc Thr 400 att Ile ctc Le u 9 cg Al a ctt Leu cg t Arg 290 ttc Phe gac Asp gac Asp gag Glu tgg Trp 370 cac His aaa Lys ccc Pro gac Asp atg Met tct Se r 275 gcg Ala 99C Gly aca Thr atc le gCC Ala 355 aac As n t cc Ser ga c Asp 9 ca Ala 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1372 <210> 88 <211> 421 <212> PRT <213> Corynebacterium glutamicum <400> 88 Met Lys Val Asn Leu Gly Ile Gly Ser Tyr Pro Arg Arg Arg Ala Thr wo 01/00805 WO 0100805PCTJIBOO/00926 1 Val1 Tyr Asp His Th r Ile Ile Val1 Ile 145 Asp GI y Le u Pro Met 225 Val1 Pro Asp Giu Gi y 305 Thr Al a Arg Gin Val Pro Al a Le u Giu 135 Al a Tyr Arg Giu Giy 215 Ile Arg Le u Ala Trp 295 Thr Le u Ile Glu Val Asn Asp Arg Phe Phe Arg Leu Ser Ile 105 Gin Glu 120 Gly Pro Asp Ile Thr Ser Asn Leu 185 Gin Giy 200 Leu Asn Ile His Ser Lys Ser Asn 265 Ile Met 280 Gin Gly Ala Pro Giy* Thr Glu Leu Ala Thr 75 Ala Phe His Leu Le u 155 Thr Phe Val Th r Giu 235 Ser Pro Gly Asp Al a 315 Gly Giy Giy Tyr Asn As n Ty r Pro Arg 140 Glu Thr As n Leu Thr 220 Phe Glu Ser Gly ValI 300 Cys Arg Ile Leu Phe Giy Asp Asp Ile Ala Asp Pro 175 Asn Thr Val1 Gi u Met 255 Glu Leu Val1 Giu Phe 335 Thr Leu As n Al a T yr Le u Met Leu Asp 160 Lys Ala Aia Met His 240 Val Arg Ser Gin Al a 320 Phe Thr Gly Tyr Tyr 385 Ilie Tyr WO 01/00805 Asp Ile L~ Glu Ala GI 355 Trp Asn Ai 370 His Ser G] Lys Asp Ar Pro Ala GI 42 PCT/IBOO/00926 es -y

-U

Le u Giu Pro Asp Ile 405 ValI Val Asp Pro Lys 345 Val Leu Ile Arg 360 Giu Asp Thr Ala 375 Ile Ala Ile Lys 390 Lys Asp Met Tyr Giy Glu Glu Val 350 Pro His Val Met 365 Ala Leu Gin Asn 380 Giu Asp Gly Tyr 395 Ser Giy Gly Glu Pro Thr Thr Giy Gly Trp Tyr Thr .400 Asn Ile 415 <210> 89 <211> 867 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (844) <223> RXA00681 (400> 89 aagggcaagg ttgtcattgt tgatccagat gcgccacgca aqccatgcgc actcagtagc agcacatggg gtggatacac act Thr gaa Giu ctg Leu gat Asp gcc Aia ctt Le u gCC Al a gga Gly acc Th r gtt Val gct Ala ata Ile tcgaaggacc cggcgcgcac atg cct agt cca cgc Met Pro Ser Pro Arg 1 ggt cgg gca ttc gct Gly Arg Ala Phe Ala gtg gcg gat atc aat Val Ala Asp Ile Asn aaa gca ttg ggc gca Lys Ala Leu Gly Ala ttg gag tcc acc gag Leu Giu Ser Thr Glu ggc cga att gat gtc Gly Arg Ile Asp Val act cgt tca ccg ttt Thr Arg Ser Pro Phe 100 115 163 211 259 307 355 403 WO 01/008 gag gat att PCTIBOOIOO926 oto 451 Le u ga c Glu aaa Lys aa t Asn gca Ala 150 acc Thr g og Ala gat Asp agc Ser gaa Giu 230 Asp gyc Gly gc C Ala 135 cac His agg Arg gt t Val1 agc Ser caa Gin 215 gc Ala Ile Asp 105 ccg tgg Pro Trp 120 cgt gtg Arg Val tgg gcg Trp Ala gtg ctt Val Leu gog cot Ala Pro 185 qcq gaa Ala Giu 200 cog aaa Pro Lys qaa tac Glu Tyr oct gog gag tgg gat Pro Ala Giu Trp Asp 110 ttg gtc atg gga gtc aat Leu Val Met Gly Val Asn ttg gtg acg Leu Val Thr gtc aat ott Val Asn Leu 140 cac tao gtg His Tyr Val 155 got aaa gag Ala Lys Glu 170 ggg ttt acg Gly Phe Thr acg tac ggt Thr Tyr Gly gao ato gtc Asp Ile Val 220 atc act ggg Ile Thr Gly 235 og t Arg 125 too Ser gca Ala o tg Leu ctg Leu gt 0 Val1 205 ggc Gly caa gtg ValI gog Al a aaa Lys gg t Giy 175 gaa Glu ogo Arg acc Thr ctc agt Ser act Thr gg t Gly 160 ogt Arg gc Al a gg a Giy atg Met cog Pro gtg Val1 145 ggg Gly ggg GI y ago Ser too Ser ttt Phe 225 ttt Phe 130 ttt Phe gt o ValI ato Ile t tg Leu ato Ile 210 ott Le u ttg Leu t oa Se r att Ile acg Thr gga Gi y 195 aag Ly s g ca Ala ga t Asp tot Se r tta Le u 165 aat Asn atg Met gca Al a oca Pro ato gtt gat ggt ggc cga Gin Thr Leu Ile Val Asp Gly Giy 240 cag tto ato taagtaotaa aagttotaag gag Gin Phe Ile <210> <211> 248 <212> PRT <213> Corynebacterium giutamicun <400> Met Pro Ser Pro Arg Thr Vai Leu Ile Thr 1 5 10 Gly Arg Ala Phe Ala Giu Gly Phe Ala Ala 25 Val Ala Asp Ile Asn Leu Asp Giy Ala Gln 40 Lys Ala Leu Giy Ala Asp Aia Ala Ala Phe 55 Leu Giu Ser Thr Glu Ala Leu Aia Ala Giy 70 Ala Ala Gly Gly Leu Gly Asp Arg Ile Aia Thr Val Asp Lys Leu Val Asp Val Thr Ser Ala Giu Phe Giy Giy WO 01/00805 WO 0100805PCTIBOOIOO926 Gly Thr Met Pro Val1 145 Gly Gly Ser Se r Phe 225 Val1 Arg Arg Giy Phe 130 Phe ValI I le Leu Ile 210 Leu Asp Ile Ser Val1 115 Le u Se r Ile Thr Gly 195 Lys Al a Gly Asp Pro 100 Asn Ser Gly Ala Val1 180 Le u Arq Ser Gly Val Phe Leu Asp Se r Leu 165 Asn Met Ala Pro Arg 245 Leu Glu Lys Asn Al a 150 Thr Al a Asp Se r Giu 230 Gin Ala Pro Leu ValI His Ala 170 Gly Thr Asp Ile Al a Ala Val Asn Tyr 155 Lys Phe Tyr Ile Thr 235 Ile Giu Thr Le u 140 Val Glu Thr Gly Val 220 Gly T yr Trp Arg 125 Ser Ala Le u Le u Val 205 Gly Gin Ala Asp 110 Ser Se r Se r Gly Th r 190 Asp Th r Th r <210> 91 <211> 837 <212> DNA <213> Corynebacterium giutamicum (220> <221> CDS <222> (814) <223> RXA00802 (400> 91 gccatggtga tcacaccagt gtgggggctg aggctccaca gcgggcacta agccccgaac gattagtagg cttgggcacc ggt ggc caa Gly Gly Gin gaa gtt gta Giu Val Val tcg aga agc gtc ata ctt gtt gtt ggc ggt gca Val Ile Leu Val Val Gly Gly Ala 15 aaa ctc tta act gaa gaa ggc gca Lys Leu Leu Thr Giu Giu Gly Ala 30 acg ccc tta tct att gac gct tcg cttggaggaa tggattaaac atg gat ctt aaa ctt Met Asp Leu Lys Leu 1 gga act att ggt tct Gly Thr Ile Gly Ser acc gcg gta gcg gcg Thr Ala Val Ala Ala gat gaa gcg tcc gtc WO 01/00805 PCTIIBOO/00926 Ser Arg Ser Thr Pro Leu Ser Ile Asp Ala Ser Asp Giu Ala Ser Val cgt Arg ctg Le u g ca Ala acg Thr gga Gly ttg Le u aaa Lys 150 att Ile gca Ala tgt Cys gac Asp aag Lys 230 att ga t Asp tct Se r gac Asp gct Ala atc Ile gcg Ala ga t Asp ttc Phe 170 acc Thr gca Al a agg Arg cag Gin gca Al a 75 act Thr g ca Ala gtt Val1 t cg Se r cg t Arg 155 gtg Val1 a cg Thr ccg Pro gca Ala gtg ValI ccc Pro qtg Val1 acc Thr gca Al a g cg Ala 140 cac His gta Val t gg T rp cat His t ca Se r 220 atc Ile gct Al a t tg Le u gtg Vali ctt Leu 125 cga Arg g cg Ala gct Ala a gg Arg caa Gin 205 t cc Ser g ca Ala g cg Ala gct Ala gcg Ala 110 tcc Se r aat As n gg C Gly gag Giu agg Arg 190 ttt Phe ttc Ph e gaa cat ggt cgc Glu His Gly Arg caa acg ctc agc Gin Thr Leu Ser 80 gct att gaa ggc Ala Ile Glu Gly 95 ctc gag aag atg Leu Giu Lys Met ggc atg aac tca Gly Met Asn Ser 130 gct gcg ctg aat Ala Ala Leu Asn 145 acc gga att aca Thr Gly Ile Thr 160 cca gac gct gag Pro Asp Ala Giu 175 tcg cgg agg cga Ser Arg Arg Arg ctg gag aga tta Leu Giu Arg Leu 210 ctt agc tcg cgt Leu Ser Ser Arg 225 ctg gat ggg Leu Asp Gly gcg gag aca Ala Giu Thr aaa gcc atc Lys Ala Ile .100 cgt gag gct Arg Glu Ala 115 tac aaa aca Tyr Lys Thr gtc gtg gtg Val Val Val gta aat gcg Val Asn Ala 165 gta aac cgc Val Asn Arg 180 tcg cgt ttt Ser Arg Phe 195 ttt cgg tgg Phe Arg Trp gag ctt ccc Giu Leu Pro 307 355 403 451 499 547 595 643 691 739 787 cgt aag cac ccc cgt gtg agg gca Arg Lys His Pro Arg Val Arg Ala 235 taacggccgt tctgttaaag (210> 92 <211> 238 <212> PRT <213> Corynebacteriun giutamicum <400>.92 Met Asp Leu Lys Leu Gly Gly Gin Val Ile Leu Val Val Gly Gly Ala wo 01/00805 PCT/IBOO/00926 1 5 10 Gly Thr Ile Gly Ser Giu Val Val Lys Leu Leu Thr Glu Giu Gly Ala 25 Thr Ala Vai Ala Ala Ser Arg Ser Thr Pro Leu Ser Ile Asp Ala Ser 40 Asp Giu Ala Ser Vai Arg Ala Gly Ile Asp Gin Val Ile Ala Glu His 55 Giy Arg Leu Asp Gly Leu Val Vai Ser Ser Ala Pro Ala Ala Gin Thr 70 75 Leu Ser Ala Giu Thr Ala Asp Asp Pro Asp Thr Val Leu Ala Ala Ile 90 Glu Gly Lys Ala Ile Thr Phe Met Lys Ala Ala Thr Val Ala Leu Glu 100 105 110 Lys Met Arg Glu Ala Gly His Gly Arq Ile Val Ala Leu Ser Gly Met 115 120 125 Asn Ser Tyr Lys Thr Leu Ser Thr Thr Ala Ser Ala Arg Asn Ala Ala 130 135 140 Leu Asn Val Val Vai Lys Asn Leu Ala Asp Arg His Ala Gly Thr Gly 145 150 155 160 Ile Thr Vai Asn Ala Ile Ser Pro Gly Phe Val Val Ala Glu Pro Asp 165 170 175 Ala Giu Val Asn Arg Aia Asn Giy Asp Thr Thr Trp Arg Arg Ser Arq 180 185 190 Arq Arg Ser Arg Phe Cys Cys Arg Arg Ala Pro His Gin Phe Leu Giu 195 200 205 Arg Leu Phe Arg Trp Asp Ile Arg Arg Arg Ala Ser Ser Phe Leu Ser 210 215 220 Ser Arg Giu Leu Pro Lys Arg Lys His Pro Arg Val Arg Ala 225 230 235 <210> 93 <211> 329 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (306) <223> RXA02133 <400> 93 gaa aac ccc tac att ggc gga gct gga tac aac gca gca aaa ttc ggt 48 Giu Asn Pro Tyr Ile Gly Gly Ala Gly Tyr Asn Ala Ala Lys Phe Gly 1 5 10 gta gca gca ttc aac cgt gtg ctt cgc ttg gaa acc cac cag cag acc 96 WO 01/00805 Ala Ala Phe cgc gta tct Arg Val Ser ctc gtt cgt Leu Val Arg gac gtc ctc Asp Val Leu gtc gcg agc Val Ala Ser cct cgc gat Pro Arg Asp 100 As n gag Glu ttc Phe aac As n ctt Le u cag Gin Arg atc Ile Gly ctc Leu cca Pro gtc Val1 Val Leu Arg Leu Giu Thr His 25 gat cca ggt cga gtt gcc acg Asp Pro Gly Arg Val Ala Thr 40 gga gat aaa gaa cgc gca gaa Gly Asp Lys Glu Arg Ala Glu 55 acc gct gaa gac atc gca gag Thr Ala Giu Asp Ile Ala Glu 75 aag cac atg aac att gac cgc Lys His Met Asn Ile Asp Arg 90 taaaacccgc actcttttga aat Gin gaa Glu g ca Al a t ct Ser.

a tg Met Gin gaa Glu qtc Val1 gtg Val1 cg t Arg PCTIIBOO/00926 Thr ttc 144 Phe tat 192 Tyr cgt 240 Arg att 288 Ile <210> 94 <211> 102 <212> PRT <213> Corynebacterium glutamicui <400> 94 Glu Asn Pro Tyr Ile Gly Gly Ala Gly 1 Val Ala Ala Phe Asn Arg Val Leu Arg 25 Leu Arg Val Ser Glu Ile Asp Pro Gly 40 Ser Leu Val Arg Phe Gly Gly Asp Lys 55 Asp Asp Val Leu Asn Leu Thr Ala Glu 70 Trp Val Ala Ser Leu Pro Lys His Met Thr Pro Arg Asp Gin Val 100 <210> <211> 1347 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1324) <223> RXN01114 Al a Thr Ala Ala Al a Asp Al a His Thr Glu G iu Arg Lys Gin Glu Ala Ser Met Gly Th r Phe Tyr Arg Ile WO 01/00805 <400> tteggtggaa gatatecgca agctggtgct geegcagett PCT/IBO0100926 ttagaaaegg ctcaageaat ttegacagat etctctgcae tctaaattaa ggateaaaaa atg aac ect eaa Met Asn Pro Gin gtc Val1 tte Phe atc Ile etc Leu gt t Val gat Asp cac His tcc Se r gtc Val 135 ga a Giu ac Thr gac Asp g eq Ala a ag ate Ile acc Thr gtt Val1 gge Gly get Ala eq c Arg gtt Val1 atg Met 120 aag Ly s acc Thr get Al a aag Lys ggg Gly 200 ege tgt Cys qgc Gly gag Glu cag Gin eta Leu eg C Arg gca Ala 105 a gc Ser ggc Gly gct Ala gag Giu atc Ile 185 ctt Leu gge tee Se r gtc Val1 gca Ala gca Ala ga t Asp tgt Cys tee Se r cge Arg gg C Gi y Gly aac Asn 170 tee Ser ttc Phe cag eca Pro cct Pro ac Thr tee Se r tcc Se r gg t Gly ggc Gly gt t ValI ga c Asp 9ga Gly 155 ctg Le u gca Ala gac Asp gag t tg Le u gt t Val1 Gly ec Pro 60 aag Lys tcc Ser get Ala gag Giu atg Met 140 ege Arg cgt Arg 9g Ala gac Asp cca cge Arg ga a Giu atc Ile 45 aac Asn ctt Le u ggc Gly get Ala tae T yr 125 cag Gin aa c Asn eg C Arg tcc Se r gag Giu 205 ace ace Thr ga a Glu 30 acc Thr gg t Gly ggc Gly ctg Leu gat Asp 110 ace T hr ctt Leu cac His gaa Glu cag Gin 190 atc Ile atc eca Pro 15 t tg Leu gg C Gly gcg Ala ca a Gin cag Gin 95 ctg Leu gtg ValI cg t Arg ceg Pro tac Tyr 175 cag Gin gtg Val gtt gtt Val gcc Ala gac Asp get Ala aac Asn 80 g ca Ala atc Ile tee Ser gac Asp ate Ile 160 gge Gly cg t Arg eca Pro tct 1 gg t Gly ace Thr gat Asp eca Pro gt t Val1 ate Ile atc Ile ggc Gly cge Arg 145 ce t Pro ate Ile tgg Trp gtc Val cga get Al a ace Thr gtg Val1 gca Ala e ca Pro gte ValI gca Ala gat Asp 130 ctt Le u ggt Gly tee Ser ggc Gly ace Th r 210 gac tac Tyr g tg Val ga c Asp ctg Leu ggc Gly ace Th r gg t Gly 115 ate Ile gca Ala ggc Gly eq e Arg aag Lys 195 gte Val1 gag ggc Gly ate Ile gat Asp gge Gly at g Met get Ala 100 gge GI y eg t Arg gaa Glu atg Met gag Giu 180 get Al a ect Pro eat gat Asp gg a Gly aac As n etg Le u egt Arg eag Gin get Al a gea Al a t gg Trp gea Ala ate Ile 165 gag Giu get Al a gee Ala gg t 115 163 211 259 307 355 403 451 499 547 595 643 691 739 787 WO 01/00805 PCT/IBOO/00926 Lys Lys Arg Gly Gin Glu Pro Thr Ile Val Ser Arg Asp Giu His Gly 215 220 225 cga cca gga aca acc gtc gaa aag ctt gct gct ttg cgc ccc ate atg 835 Arg Pro Gly Thr Thr Val Giu Lys Leu Ala Ala Leu Arg Pro Ile Met 230 235 240 245 qgc cgc cag gat gcg gaa gca acc gtc acc gct ggc aac gcg tcc ggc 883 Gly Arg Gin Asp Ala Giu Ala Thr Val Thr Ala Gly Asn Ala Ser Gly 250 255 260 caa aat gat ggc gct gct gcc gtc atc gtg acc act cgc gcc aag gcc 931 Gin Asn Asp Gly Ala Ala Ala Val Ile Val Thr Thr Arg Ala Lys Ala 265 270 275 gag gag aag ggc etg cgc cca gtc atg cgt ttg gct ggc tgg tct gtg 979 Giu Giu Lys Gly Leu Arg Pro Val Met Arg Leu Ala Gly Trp Ser Val 280 285 290 get gct git ccc cca gag acc atg ggt att gga cct gtt cet gcc acc 1027 Ala Ala Val Pro Pro Giu Thr Met Giy Ile Gly Pro Val Pro Ala Thr 295 300 305 aag aag gte ctg gat cgt ttg ggc ctt acc ctg gag gac atc ggc gcg 1075 Lys Lys Val Leu Asp Arg Leu Gly Leu Thr Leu Giu Asp Ile Gly Ala 310 315 320 325 ate gaa etc aac gaa gct ttc gca gct cag gca ctg tct gtg ctg aag 1123 Ile Glu Leu Asn Giu Ala Phe Ala Ala Gin Ala Leu Ser Val Leu Lys 330 335 340 gaa tgg aac at t tct tgg gaa gat gag cgc gtc aac cca ctg ggt tee 1171 Glu Trp Asn Ile Ser Trp Glu Asp Glu Arg Val Asn Pro Leu Gly Ser 345 350 355 ggt att tcc atg gga eac cca gte ggt gcc ace ggt gct ege atg gca 1219 Gly Ilie Ser Met Gly His Pro Val Gly Ala Thr Gly Ala Arg Met Ala 360 365 370 gta ace ttg get cac ege atg cag egt gaa aac act cag tac gga ctg 1267 Val Thr Leu Ala His Arg Met Gin Arg Giu Asn Thr Gin Tyr Gly Leu 375 380 gee ace atg tgc ate ggt ggc ggc cag ggt ctt gca get gte ttt gaa 1315 Ala Thr Met Cys Ilie Gly Gly Gly Gin Giy Leu Ala Ala Val Phe Giu 390 395 400 405 aag gag aac taaaaatggc tattttgcac age 1347 Lys Glu Asn <210> 96 <211> 408 <212> PRT (213> Corynebacterium glutamicum <400> 96 Met Asn Pro Gin Asp Ile Val Ilie Cys Ser Pro Leu Arg Thr Pro Val 1 5 10 wo 01/00805 WO 0100805PCT/IBOO/00926 Gly Thr Asp Pro Val1 Ile Ile Gly Arg 145 Pro Ile Trp Val1 Arg 225 Leu Gly Thr Ala Pro 305 Glu Leu Ala Th r Val Ala Pro Val Al a Asp 130 Le u Gly Ser Gly Thr 210 Asp Arg Asn Arg Gly 290 Val1 Asp Se r Tyr Val Asp Le u Gly Th r Gly 115 Ile Ala Gly Arg Lys 195 Val Glu Pro Al a Ala 275 Trp Pro Ile Val1 Gly Ile Asp Gly Met Al a 100 Gi y Arg Glu Met Gi u 180 Al a Pro His Ile Se r 260 Lys Ser Al a Gly Le u Gi y As n Le u Arg Gin Ala Ala Trp Ala Ile 165 Glu Ala Al a Gly Met 245 Gly Al a Val Thr Al a 325 Lys Ser Ala Ile Val1 70 Leu Ala Glu Gly Arg.

150 Glu Gin Asp Lys Arg 230 Gly Gin Glu Ala Lys 310 Ile Glu Phe Ile Leu Val1 As p His Ser Val1 135 Glu Thr Asp Al a Lys 215 Pro Arg Asn Glu Ala 295 Lys Glu T rp Thr ValI 40 Gly Ala Arg Val1 Met 120 Lys Thr Ala Lys Gly 200 Arg Gly Gin Asp Lys 280 Val Val1 Le u As n ValI Ala Ala Asp Cys 90 Se r Arg Gly Gly As n 170 Ser Phe Gin Thr Ala 250 Ala Leu Pro Asp Glu 330 Ser Pro Th r Se r Se r 75 Gly Gly ValI Asp Gly 155 Le u Ala Asp Glu Val1 235 Giu Ala Arg Glu Arg 315 Al a Trp Val Gly Pro Lys Se r Ala Giu Met 140 Arg Arg Al a Asp Pro 220 Glu Al a Ala Pro Th r 300 Le u Phe Glu Glu Ile Asn Leu Gly Al a T yr 125 Gln As n Arg Ser Giu 205 Thr Lys Thr.

Val ValI 285 Met Gly Ala Asp Glu Thr Gly Gly Leu Asp 110 Thr Leu His Glu Gin 190 Ile Ile Le u Val Ile 270 Met Gly Le u Ala Glu Leu Gly Al a Gin Gin Le u Val1 Arq Pro Tyr 175 Gin Val Val1 Ala Thr 255 Val1 Arg Ile Thr Gin 335 Arg Ala Asp Al a Asn Al a Ile Se r Asp Ile 160 Gly Arg Pro Se r Ala 240 Al a Thr Leu Gly Leu 320 Ala Val WO 01/00805 WO 0100805PCTI[BOO/00926 340 Asn Pro Leu Gly Ser 355 Gly Ala Arg Met Ala 370 Thr Gin Tyr Giy Leu 385 Ala Ala Val Phe Glu 405 345 350 Gly Ile Ser Met Gly His Pro Val Gly Ala Thr 360 365 Vai Thr Leu Ala His Arg Met Gin Arg Glu Asn 375 380 Ala Thr Met Cys Ile Gly Giy Gly Gin Gly Leu 390 395 400 Lys Giu Asn <210> 97 <211> 815 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> <223> FRXA01114 <400> 97 cgc ctt gca Arg Leu Ala 1 cct ggt ggc Pro Gly Gly atc tcc cgc Ile Ser Arg tgg ggc aag Trp Giy Lys gtc acc gtc Val Thr Vai cga gac gag Arg Asp Giu ttg cgc ccc Leu Arg Pro ggc aac gcg Gly Asn Aia 115 act cgc gcc Thr Arg Ala 130 gaa Glu atg Met gag Giu gc t Aila cct Pro cat His atc Ile 100 tcc Ser aag Lys gca cgc gaa acc Aia Arg Giu Thr atc gag acc gct Ile Giu Thr Ala gag cag gac aag Giu Gin Asp Lys gct gat gcg ggg Aia Asp Ala Gly gcc aag aag cgc Ala Lys Lys Arg 70 ggt cga cca gga Gly Arg Pro Gly atg ggc cgc cag Met Giy Arg Gin ggc caa aat gat Gly Gin Asn Asp 120 gcc gag gag aag Ala Giu Giu Lys 135 gct ggc gga Aia Gly Giy cgc aac cac ccg atc Arg Asn His Pro Ile gag Giu 25 atc Ile ctt Le u ggc Gi y aca Thr ga t Asp 105 ggc Giy gg C Gly aac Asn tcc Se r ttc Phe cag Gin acc Thr 90 gcg Aia gct Ala ctg Leu gaa Giu cag Gin atc Ile atc Ile ctt Leu gtc Val1 110 atc Ile atg Met tac Tyr cag Gin gtg Vali gtt ValI gct Ala acc Thr gtg Val cg t Arg ggc Gly cg t Arg cca Pro tct Ser gct Al a gc t Ala acc Thr ttg Le u WO 01/00805 WO 0100805PCT/IBOO/00926 gct ggc tgg tct gtg gct gct gtt ccc cca gag acc atg ggt att gga 480 Ala Giy Trp Ser Vai Ala Ala Val Pro Pro Gbu Thr Met Gly Ile Giy 145 150 155 160 cct gtt cct gcc acc aag aag gtc ctg gat cgt ttg ggc ctt acc ctg 528 Pro Val Pro Ala Thr Lys Lys Val Leu Asp Arg Leo Giy Leo Thr Leo 165 170 175 gag gac atc ggc gcg atc gaa ctc aac gaa gct ttc gca gct cag gca 576 Glu Asp Ile Gly Ala Ile Glu Leu Asn Glu Ala Phe Ala Ala Gin Ala 180 185 190 ctg tct gtg ctg aag gaa tgg aac att tct tgg gaa gat gag cgc gtc 624 Leu Ser Val Leo Lys Glu Trp Asn Ile Ser Trp Giu Asp Glu Arg Val 195 200 205 aac cca ctg ggt tcc ggt att tcc atg gga cac cca gtc ggt gcc acc 672 Asn Pro Leu Gly Ser Giy Ile Ser Met Gly His Pro Val Gly Ala Thr 210 215 220 ggt gct cgc atg gca gta acc ttg gct cac cgc atg cag cgt gaa aac 720 Gly Ala Arg Met Ala Val Thr Leo Ala His Arq Met Gin Arg Gbu Asn.

225 230 235 240 act cag tac gga ctg gcc acc atg tgc atc ggt ggc ggc cag ggt ctt 768 Thr Gin Tyr Gly Leo Ala Thr Met Cys Ile Gly Gly Gly Gin Giy Leo 245 250 255 gca gct gtc ttt gaa aag gag aac taaaaatggc tattttgcac agc 815 Ala Ala Val Phe Glu Lys Glu Asn 260 <210> 98 <211> 264 <212> PRT <213> Corynebacterium giotamicom <400> 98 Arg Leo Ala Gbu Ala Arg Glu Thr Ala Gly Gly Arg Asn His Pro Ile 1 5 10 Pro Gly Gly Met Ile Gbu Thr Ala Glu Asn Leo Arg Arg Gbu Tyr Gly 25 Ile Ser Arg Gbu Gb Gin Asp Lys Ile Ser Ala Ala Ser Gin Gin Arg 40 Trp Gly Lys Ala Ala Asp Ala Gly Leo Phe Asp Asp Gu Ile Val Pro 55 Val Thr Val Pro Ala Lys Lys Arg Gly Gin Gbu Pro Thr Ile Val Ser 70 75 Arg Asp Glu His Gly Arg Pro Gly Thr Thr Val Gbu Lys Leo Ala Ala 90 Leo Arg Pro Ile*Met Gly Arg Gin Asp Ala Gio Ala Thr Val Thr Ala 100 105 110 wo 01/00805 WO 0100805PCTIBOO/00926 Gly Thr Al a 145 Pro Glu Le u As n Gly 225 Th r Al a Asn Arg 130 Gly Val1 Asp Se r Pro 210 Ala Gln Ala Ala 115 Ala Trp Pro Ile Val1 195 Le u Arg Tyr Val Ser Lys Se r Ala Gly 180 Le u Gl y Met Gl y Phe 260 Al a Pro 140 Thr Le u Phe Glu Pro 220 Met Gly Val1 125 Val1 Met Gly Ala Asp 205 Val Gln Gly Ile Met Gly Leu Ala 190 Glu Gly Arg Gln Val Arg Ile Thr 175 Gln Arg Ala Glu Gly 255 <210> 99 <211> 978 <212> DNA <213> Corynebacterium glutamicun <2 <221> CDS <222> (101) (955) <223> RXA01894 (400> 99 agaatttttt cgaaaatgct ggcaccatca acagtgacat acccatgaat gaaccggagc aacatcaccg gtccatgagg aat Asn ctg Le u tac Tyr agc gcg Al a gtc Val ctc Le u ctt att Ile cta Le u gca Ala cca tgttagaaac ttcaaggaga atq ccc aaa ccc aaa Met Pro Lys Pro Lys 1 gct gtg ggg atc gga Ala Val Gly Ile Gly agc cca tgg ggt tgg Ser Pro Trp Gly Trp aca tgg gaa gtt ggt Thr Trp Glu Val Gly ctg ccg att atg atc 115 163 211 259 307 WO 01/00805 WO 0100805PCTIBOO/00926 Ser Arg Leu atc ggc ggt Ile Gly Gly ggC att ttg Gly Ile Leu cga att ttc Arg Ile Phe gac acc tct Asp Thr Ser 120 agc ttc qct Ser Phe Ala 135 aca tat ttc Thr Tyr Phe 150 ggc ggg tat Gly Gly Tyr ttg qtg agt Leu Val Ser tta qga tcg Leu Gly Ser 200 cac tgg tgg His Trp Trp 215 acg ttg ggt Thr Leu Gly 230 aag gat atg Lys Asp Met ttg gat ggc Leu Asp Gly gtg atc agc Val Ile Ser 280 <210> 100 Lys Giu Gly cag Gin 9 cg Ala tac Tyr 105 gtq Val1 gcg Aia att Ile atc Ile ccg Pro 185 gtc Vali atg Met qac Asp tcg Se r atg Met 265 gca Ala tct Ser aat Asn Giy atg Met ttg Leu g cg Ala 170 aag Lys act Thr ggt Gly t tq Leu aac Asn 250 ctc Le u atc Ile 75 ttt Phe 99C Gly atc Ile Ctg Le u acg Thr 155 99 t Gly aag Lys gg t Gly gtg ValI gtt Val1 235 ctt Leu ccg Pro Gly 60 atc Ile gtg Val1 acg Thr ttc Phe t cg Se r 140 ttc Phe gtg Vali tct Ser gca Ala atc Ile 220 gag Giu ctt Le u gcc Ala Tyr His Leu Pro Leu Pro Ile Met Ile tgg ctg Trp Leu gcc act Ala Thr gaa aaa Giu Lys 110 gtg ctc Val Leu 125 ctg atq Leu Met atg ctg Met Leu ttc ttt Phe Phe tgg gaa Trp Giu 190 ctc agt Leu Ser 205 ttg ggt Leu Giy tcg cag Ser Gin cca ggc Pro Giy gcg atg Ala Met 270 tca tgg Ser Trp 80 gtg ttg Vai Leu 95 gaa gcc Glu Ala acc tgg Thr Trp caa aac Gin Asn tgt gtg Cys Val 160 gga tcg Gly Ser 175 ggc ttt Gly Phe qtt cac Val His tgt gcc Cys Aia ttc aaa Phe Lys 240 cac ggc His Gly 255 gtg acg Val Thr cca Pro gtg ValI cgC Arg att Ile aat Asn 145 atc Ile cac His gcc Ala ttc Phe cta Le u 225 cg c Arg gga Gly tgg Trp ttt Phe ctg Le u aac Asn cca Pro 130 tcc Se r gca Ala cca Pro 99c Gly ctg Leu 210 gtt Vai gat Asp ttg Le u ttg Leu ggc Gly a tg Met tat Tyr 115 ttg Leu atc Ile t cq Ser a tg Met tcC Ser 195 ctc Leu gtg Vali ttg Leu a tg Met atc Ile 275 acg atg Thr Met tat ttc Tyr Phe 100 ttg agg Leu Arg ttc gga Phe Giy ccg ggt Pro Gly gat gtg Asp Vai 165 gcg ccg Ala Pro 180 att gtc Ile Val qat cac Asp His tgc gcc Cys Ala ggc atc Gly Ile 245 gac cgt Asp Arg 260 ctg agt Leu Ser agc tcg tat ccg Ser Ser Tyr Pro taaagcttgg gccagcttta agt WO 01/00805 PCT/IB00/00926 <211> 285 <212> PRT <213> Corynebacterium glutamicum <400> 100 Met Pro Lys Pro Lys Asn Asn Ala Gly Arg Asp Leu Lys Ala Ala Ile 1 5 10 Ala Val Gly Ile Gly Leu Gly Val Leu Val Leu Leu Gly Ile Val Leu 25 Ser Pro Trp Gly Trp Tyr Ile Leu Val Ala Gly Phe Met Ala Ala Ala 40 Thr Trp Glu Val Gly Ser Arg Leu Lys Glu Gly Gly Tyr His Leu Pro 55 Leu Pro Ile Met Ile Ile Gly Gly Gin Ala Ile Ile Trp Leu Ser Trp 70 75 Pro Phe Gly Thr Met Gly Ile Leu Ala Ser Phe Val Ala Thr Val Leu 90 Val Leu Met Tyr Phe Arg Ile Phe Tyr Asn Gly Thr Glu Lys Glu Ala 100 105 110 Arg Asn Tyr Leu Arg Asp Thr Ser Val Gly Ile Phe Val Leu Thr Trp 115 120 125 Ile Pro Leu Phe Gly Ser Phe Ala Ala Met Leu Ser Leu Met Gin Asn 130 135 140 Asn Ser Ile Pro Gly Thr Tyr Phe Ile Leu Thr Phe Met Leu Cys Val 145 150 155 160 Ile Ala Ser Asp Val Gly Gly Tyr Ile Ala Gly Val Phe Phe Gly Ser 165 170 175 His Pro Met Ala Pro Leu Val Ser Pro Lys Lys Ser Trp Glu Gly Phe 180 185 190 Ala Gly Ser Ile Val Leu Gly Ser Val Thr Gly Ala Leu Ser Val His 195 200 205 Phe Leu Leu Asp His His Trp Trp Met Gly Val Ile Leu Gly Cys Ala 210 215 .220 Leu Val Val Cys Ala Thr Leu Gly Asp Leu Val Glu Ser Gin Phe Lys 225 230 235 240 Arg Asp Leu Gly Ile Lys Asp Met Ser Asn Leu Leu Pro Gly His Gly 245 250 255 Gly Leu Met Asp Arg Leu Asp Gly Met Leu Pro Ala Ala Met Val Thr 260 265 270 Trp Leu Ile Leu Ser Val Ile Ser Ser Ser Tyr Pro Ser 275 280 285 <210> 101 WO 01/00805 <211> 600 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (577) <223> RXA02599 <400> 101 gatcaagcat catacgatgt cggagattgt ggcgggcgtt PCTJIBOO/00926 gttacaggag cagttgcaac cggcatttgt tatgcactcc tacttgcgta aaggagaatt atg gat cag cta atc Met Asp Gin Leu Ile ctc Le u att Ile ctt Le u ggg Gly cc t Pro ttc Phe ggg Gly ct 9 IA u ga t Asp gg C Gly ctc Leu ttg Le u atc Ile gca Ala gCt Ala cgg Arg tct Ser t gg T rp ttg Le u acc Thr cgc Arg agt Ser gag Giu gtg Val tgg Trp cgq Arg gcc Al a 140 ccg Pro gtg Val1 atc Ile ttt Phe aac As n gt g ValI gt a Val 115 t gg T rp gtg gtg Val tat Tyr gcg Al a ga a Glu ttt Phe gca Al a 100 atc Ile

CCC

Pro gtg Leu Thr Ser Val Val Val 145 ttt acc agc tgg aac ctc ctc cag cgc cgc tgaaaccact gctgaaagtg Phe Thr Ser Trp Asn Leu Leu Gin Arg Arg 150 155 gta <210> 102 <211> 159 WO 01/00805 (212> PRT <213> Corynebacterium giutamicum <400> 102 Met Asp Gin Leu Ilie Leu Asp Ala Phe Ile Gly 1 5 10 Leu Ser Pro Val Ile Ile Leu Phe Thr Gin Leu 25 Met Phe Val Tyr Ala Leu Val Trp Gly Leu Leu 40 Ala Pro Ile Ala Val Gly Leu Ala Asn Leu Ile Arg Ala Phe Giu Arg Pro Arg Pro Asn Thr Ala 70 Glu Thr Asn Phe Ser Phe Pro Ser Gly His Ala 90 Cys Ala Val Ala Val Gly Tyr Ser Val Asn Arg 100 105 Leu Trp Val Ilie Ala Leu Leu Val Gly Leu Ser 115 120 Val His Trp Pro Ser Asp Val Leu Ala Gly Trp 130 135 Thr Ser Val Val Val Phe Thr Ser Trp Asn Leu 145 150 155 <210> 103 <211> 999 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(976) <223> RXN0263B <400> 103 ctctgttgtt gttcctgagg cgcttcgtcc attcgtgggc gaaacaagcc ggttaattaa gcggaaagct cgtggtcgac PCT/IBOO/00926 Arg Gly Lys His His Gly Trp Leu 125 Ile Gin ValI Pro Ser Phe Leu Ala Lys 110 Tyr Gly Arg Th r Thr Ala Leu ValI Ala Leu Val Ala Arg ggc ttt ttc Gly Phe Phe gag tca cgg Glu Ser Arg acg gtg atg gta gag gat tcc ctg cct aag gtt Val Giu Asp Ser Leu Pro Lys Val 15 gag acg ttt tat ggg cgc atc atc Glu Thr Phe Tyr Gly Arg Ile Ile 30 aaa gcc cag gat gtg cag att tcc aaagacgtgc tcaagccagt atg gtc aaa agg ttt 115 Met Val Lys Arg Phe 1 ccg ctg cat ccc gaa 163 Pro Leu His Pro Glu att agt gct gtg cgg 211 Ile Ser Ala Val Arg atc ttc ggt gcg gag 259 WO 01/00805 WO 0100805PCTJIBOO/00926 Thr aac As n att Ili cg c Arg gtc Val1 gca Ala agc Ser at~c Ile 150 gtt Val1 aaa Lys aqc Se r ga a Glu 9C Al a 230 C cg Pro t tg Le u gtc ValI Lys ac C Thr tca Se r cga Arg ctc Leu 105 ggt Gly gg t Gly cta Le u Ctg Le u gag Glu 185 gaa Giu tac Tyr ca a Gin ctc Le u 9C Ala 265 aaa Lys Al a acc Thr ttC Phe ttC Ph e a tg Met tcc Ser atc Ile aaa Lys cca Pro 170 c gc Arg

CCC

Pro gag Giu aag Lys qgC Gly 250 gaa Glu cgc Arg Gin Gly t gg T rp a tg Met cgc Arg atg Met ttc Ph e act Th r 155 ctt Le u ga c Asp gtC Val1 gc t Al a tat Tyr 235 gg C Gly cgg Arg acc Thr Phe acc Thr gt g Val ga c Asp g tg Val CtC Le u 130 cgg Arg gac Asp cgc Arg

CCC

Pro gac Asp 210 gaa Glu ga a Glu cag Gin cgc Arg ttt Phe Gly aca Thr c gg Arg acc Thr gac Asp 115 ga c Asp t CC Ser agc Se r atc Ile gta Val 195 gaa Glu acc Thr t tg Le u gcg Ala gcg Al a 275 aaa Lys Ala ctg Leu ggt Gly cca Pro 10*0 cgc Arg gcc Ala ttt Phe gct Ala atc Ile 180 ttC Phe gca Ala cgc Arg tgg Trp aaa Lys 260 gca Al a aaa Lys WO 01/00805 PCTJIBOO/00926 280 285 290 tgattgcact gggttcagcg ccc 999 <210> 104 <211> 292 <212> PRT <213> Corynebacterium glutamicum <400> 104 Met Val Lys Arg Phe Gly Phe Phe Val Giu Asp Ser Leu Pro Lys Val 1 5 10 Pro Leo His Pro Giu Giu Ser Arg Glu Thr Phe Tyr Gly Arg Ile Ile 25 Ilie Ser Ala Val Arg Thr Val Met Lys Ala Gin Asp Val Gin Ile Ser 40 Ile Phe Gly Ala Gbu Asn Ile Pro Thr Thr Giy Gly Ala Leo Phe Ala 55 Ser Thr Thr Leu Val Ile Met Thr Ser Phe Trp Val Val Ser Pro Ala 70 75 Phe Val Arg Gly Lys Arg Leu Val Arg Phe Met Ala Lys Lys Giu Ile 90 Phe Asp Thr Pro Val Val Gly Thr Leu Met Arg Trp Met Lys His Val 100 105 110 Ser Val Asp Arg Ser Ala Giy Ala Gly Ser Met Glu Asp Ala Arg Lys 115 120 125 Arg Leu Asp Ala Gly Ser Leu Val Gly Ile Phe Pro Glu Ala Thr Val 130 135 140 Ser Arg Ser Phe Glu Ilie Lys Glu Leu Lys Thr Gly Ala Val Arg Ile 145 150 155 160 Ala Asp Ser Ala Asn Val Pro Leu Leu Pro Leu 11 Ile Trp Gly Gly 165 170 175 Gin Arg Ilie Ilie Thr Lys Asp Ile Giu Arg Asp Phe Gly Arg Ser His 180 185 190 Ile Pro Val Phe Ile Ser Val Gly Glu Pro Val Asp Ala Ser Gly Asp 195 200 205 Pro Asp Glu Ala Thr Glu Arg Leo Tyr Giu Ala Met Lys Lys Leu Leu 210 215 220 Asp Giu Thr Arg Thr Ala Tyr Giu Gin Lys Tyr Gly Pro Phe Giu Giy 225 230 235 240 Gly Glu Leu Trp Arg Pro Lys Ser Leu Gly Gly Gly Ala Pro Thr Leu 245 250 255 Glu Gin Ala Lys Met Leu Giu Ile Ala Glu Arg Giu Arg Arg Gin Ala 260 265 270 WO 01/00805 WO 0100805PCTIBOO/00926 Lys Arg Ala Ala Lys Val Ala Lys Lys Arg Thr Thr Phe Ile Arg Lys 275 280 285 Ile Phe Lys Lys 290 <210> 105 <211> 534 <212> DNA <213> Corynebacterium glutamicum <220> <221> <222> <223>

CDS

(511) FRXA02 638 <400> 105 atgaagaatg gcg cgg aag cgt ctt aac Met Ala Arg Lys Arg Leu Asn gcc ggc agc ctc gtc ggt atc Ala Gly Ser Leu Val Gly Ile ttc cct gag gcg acg Phe Pro Giu Ala Thr tca cgg tcc ttt Ser Arg Ser Phe atc aag gaa cta Ile Lys Giu Leu act ggc gcc gtc Thr Gly Ala Val cgc Arg atc gcc gac aqc Ile Ala Asp Ser aac gtt ccg ctg ctg cca Asn Val Pro Leu Leu Pro ctt att att Leu Ile Ile gac ttc ggc Asp Phe Gly ggc ggc cag cgc atc atc acc aaa gac Gly Gly Gin Arg Ile Ile Thr Lys Asp atc gag cgc Ile Giu Arg ggt gaa ccc Gly Giu Pro cgc tcc cac atc Arg Ser His Ilie gta ttc atc agc Val Phe Ile Ser gtg Val1 gtc gac Val Asp gcc agc ggc gat Ala Ser Gly Asp gac gaa gca acg Asp Giu Ala Thr cgc ctc tac gag Arg Leu Tyr Giu gct Ala atg aaa aag ctt Met Lys Lys Leu gac gaa acc cgc Asp Glu Thr Arg gcc tac gaa caa Ala Tyr Giu Gin tat ggc cca ttc Tyr Giy Pro Phe ga a Giu 115 ggt gga gaa ttg Giy Giy Giu Leu cgc ccg aaa tcc Arg Pro Lys Ser ctc ggc Leu Gly 125 qgc ggc gcc Gly Gly Ala cgg gaa cgt Arg Giu Arg 145 acg ttg gag cag Thr Leu Giu Gin aaa atg ttg gaa Lys Met Leu Giu atc gcc gaa Ile Ala Giu 140 aag aaa cgc Lys Lys Arg cga caa gca aaa Arg Gin Ala Lys gcg gca aag gtc Ala Aia Lys Val gcc Ala 155 acc acc ttt ata agg aaa atc ttt aaa aaa Thr Thr Phe Ile Arg Lys Ile Phe Lys Lys tgattgcact gggttcagcg WO 01/00805 160 PCT11B00100926 ccc <210> 106 <211> 168 <212> PRT <213> Corynebacterium giutamicum <400> 106 Met Ala Arg Lys Al a Val Trp Arg Ser Lys Phe Pro 130 Arg I le Val Ile Gly His Asp Le u 100 Gly Le u Ala Ly s Arg Ser Al a Gin Ile Pro Asp Gly Glu Lys Ile 165 Leu Asn Ala Gly Arg Ser Phe Giu Asp Ser Ala Asn Arg Ile Ile Thr 55 Pro Val Phe Ile Asp Glu Ala Thr Giu Thr Arg Thr 105 Glu Leu Trp Arg 120 Gin Ala Lys Met 135 Arg Ala Ala Lys 150 Phe Lvs Lvs Le u Lys Pro Asp Val1 Arg Tyr Lys Giu Ala 155 Val Glu Le u Ile Giy Le u GI u Se r Ile 140 Lys <210> 107 <211> 637 <212> DNA (213> Corynebacterium giutamicum <220> <221> CDS <222> (637) <223> RXA00856 <400> 107 gtttttagga atagagtggg ctcaagcttt gtgacaagtt tcttggagaa atcattacta gtcgtagtct tcaatttggg tgcaggtagg qtggaacacc gtg agt gat gta tca 115 Val Ser Asp Val Ser WO 01/00805 WO 0100805PCT1-B00100926 gca Al a tcc Se r atc Ile gct Al a gac Asp ttt Phe gca Al a gcg Ala caa Gin ad 9 Lys 150 cca Pro ggc Gly aac Asn cct Pro ttt Ph e aag Lys ggc Gly ttt Phe t tg Leo ctg Leu 135 act Thr ag t Se r gta Val1 tgg Trp ttg Leo

C

Al a ctt Leo a ag Lys gtc Val att Ile 120 cgc Arg qc t Aia t gg Trp aat ggc Asn Gly aac ctt Asn Leo ttt gcg Phe Ala tgg t99 Trp Trp gac ggc Asp Gly atc *gcq Ilie Ala tgt ttc Cys Phe 105 gtg ctt Val Leo gct gga Ala Gly ctg cag Leo Gin gca caa gat cca agc dat caa Ala Gin Asp Pro Ser Asn Gin 15 gcg gtc aag cct Ala Val Lys Pro aaC Asn ctt Leo t tg Leo 60 att I le CC9 Pro atc Ile gag Glu gtt Val 140 gtt Val1 t tg Leo 30 ctt Leu gtt ValI cga Arg gcc Ala ggc Gl y 110 99 C Gly cc t Pro gtt Val1 t t9 Leu gag Gio ttg Leo 9 gc Gly Aia ccc Pro atc Ile a ag Lys 145 tat T yr c9 t Arg acg Thr ctc Leo Ct 9 Leu t tg Leo tgg Trp t gg Trp 130 999 Gly ct g Leo atc Ile gad Gio at 9 Met 9t C Val atg Met tgg Trp 115 cgt Arg 99 C Gly tgc Cys att Ile aac Asn atc Ile act Thr dcc Thr 100 9 tc Val ttc Phe aag Lys cct Pro gt C Val dat As n dcc Thr gac Asp aca Th r act Thr ttc Phe ctt Leo ttc Phe 165 163 211 259 307 355 403 .451 499 547 595 atg gat att cca agc cag atc gtc atg tat gca Met Asp Ile Pro Ser Gln Ile Val Met Tyr Ala <210> 108 <211> 179 <212> PRT <213> Corynebacteriom giotamicom <400> 108 Val Ser Asp Vai Ser Ala Giy Val Asn Gly Ala Gln Asp Pro Ser Asn 1 5 10 Gin Ala Val Lys Pro Ser Asn Trp Asn Leu Pro Asn Phe Leo Thr Ser 25 Leo Arg Ile Ile Val Ile Pro Leo Phe Ala Trp Leo Thr Leo Lys Gly 40 Giu Le u Gi y Al a Pro Ile Ly s 145 Tyr Met WO 01/00805 Thr Glu A Leu Met I: Leu Val TI Leu Met TI

IC

Trp Trp V 115 Trp Arg Pt 130 Gly Gly L) Leu Cys Pi Tyr Ala le )0 1 :0 Asn Thr Asp Thr Thr Phe Le u Phe 165 Ala Asp 70 Phe Al a Ala Gin Lys 150 Pro Trp Asp Ile Cy s 105 Val1 Ala Le u Met Trp Gly Ala Phe Leu Gly Gin Asp 170 Ala Asp 75 Asp As n Arq Asn Thr 155 Ile Leu Ile Pro Ile Giu Val 140 ValI Pro Val1 Ala Ile Ile Phe 125 Val1 Ala Se r Val Arg Ala Giy 110 Gly Pro Val Gin PCTIiBOO/00926 Ile Arg Lys Le u Thr Ser Leu 160 Val <210> 109 <211> 780 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <222> (101)..(757) <223> RXA02511 <400> 109 ccatgcttgc gcaagcatgq ggcaccattg atggggcacc tgacttcagc gatccgtacc gcagcaccga aggagcactg g ga Giy atc Ile ctc Le u tgg Trp cag Gin a cg Th r gct Ala acg Th r gtt Val1 cct Pro ttg Le u ggC Gly aggcactgtc gacccaacqc atg ctg gga ctt cat 115 Met Leu Gly Leu His 1 gtt gcc aaa ttg atg 163 Val Ala Lys Leu Met tta gtc agc gct ggc 211 Leu Val Ser Ala Gly acg ggg cat ttg att 259 Thr Gly His Leu Ile ttc gac atq at t gat 307 Phe Asp Met Ile Asp WO 01/00805 WO 0100805PCT/IBOO/00926 ggc acg gtt qct cgc atg caa ggt ggt ggc Gly Thr Val Ala Arg Met Gin Gly Gly Gly ttg gat gcc acg tgt gac cgc atc act gat Leu Asp Ala Thr Cys Asp Arg Ile Thr Asp 95 att acc tgg tgg ctg gtg tat tec tac gat Ile Thr Trp Trp Leu Val Tyr Ser Tyr Asp 105 110 gct gcc tcC ttg gtt tgt ttq qtt gcc tcc Ala Ala Ser Leu Val Cys Leu Val Ala Ser 120 125 aaa gcc agg gga gag gcc tcc gga ttc acc Lys Ala Arg Gly Giu Ala Ser Gly Phe Thr 135 140 qaa cgc cct gag cgt ctg att gtc agc ctt Giu Arg Pro Giu Arg Leu Ile Val Ser Leu 150 155 gga atg ggc gtt cca tat gcc atc gat gtg Gly Met Gly Val Pro Tyr Ala Ile Asp Val 170 175 gct ggc agt att tac act gtt gtg cag cgc Ala Gly Ser Ile Tyr Thr Val Val Gin Arg 185 190 tcc cca ttg gct aag gaa ttt acc aag gca Ser Pro Leu Ala Lys Giu Phe Thr Lys Ala 200 205 gat tac agc aac acc aaa taaaaattag ccgac Asp Tyr Ser Asn Thr Lys 215 <210> 110 <211> 219 <212> PRT <213> Corynebacterium giutamicum <400> 110 Met Leu Gly Leu His Gly Arg Lys Pro Ala 1 5 10 Val Ala Lys Leu Met Ile Lys Leu Lys Val 25 Leu Val Ser Ala Gly Leu Thr Val Gly Val 40 Thr Gly His Leu Ile Trp Ala Ala Val Leu 55 Phe Asp Met Ile Asp Gly Thr Val Ala Arg 70 acc aaa ttt Thr Lys Phe ggt gcc acc Gly Ala Thr gg t Gi y 100 ttg Leu tac Tyr ctc Le u ct g Leu ctt Leu 180 gga Gi y aag Lys gcg Ala gtC Val gtg Vai gtg Val1 acc Th r 165 gc a Ala aaq Lys gca Ala 403 451 499 547 595 643 691 739 Pro Ala Iggagc atc Gin Thr Ala Th r Met 75 Val Ile Val Giu Pro Pro Asn Gin Leu Thr Leu Leu Leu Ile Pro Gly Leu Phe Ala Ala Gin Gly Gly Gly Thr WO 01/00805 Lys Phe Gly Ala Thr Leu Asp Ala Thr Cys Asp 90 Ala Leu Phe Gly Ala Ile Thr Trp Trp Leu Val 100 105 Pro Gin Ala Leu Val Ala Ala Ser Leu Val Cys 115 120 Val Ile Ser Tyr Val Lys Ala Arg Gly Glu Ala 130 135 Asp Gly Gly Leu Val Glu Arg Pro Glu Arg Leu 145 150 155 Gly Leu Gly Leu Thr Gly Met Gly Val Pro Tyr 165 170 Leu Trp Ala Leu Ala Ala Gly Ser Ile Tyr Thr 180 185 Val Met Ala Gly Lys Ser Pro Leu Ala Lys Glu 195 200 Ala Gly Ala Lys Ala Asp Tyr Ser Asn Thr Lys 210 215 <210> 111 <211> 678 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(655) <223> RXN02836 <400> 111 ccatcactcg gcttcaccgt ctacggtgct gcccgcagga gcttcagacg ggatccaccc cctcgagatq gacgtcaccg Thr Tyr 110 Ala Phe Ser Asp Gin 190 Lys Asp Asp Se r Thr Leu Val 175 Arg Ala PCTIIBOO/00926 Gly Ala Gin Met Val 160 Al a Leu Pro g gc Gly cg a Arg aac Asn tat Tyr gag G iu cac His ggc Gly t tc Phe gtc ValI atg Met gqg Gly gcc Ala aat Asn cag Gin aag Lys ctc Le u cagagcgccc ccaaaagctc atg aca atc gat qaa 115 Met Thr Ilie Asp Glu 1 gcg atg gcc ctc acc 163 Ala Met Ala Leu Thr tgg ggg acg atc gtg 211 Trp Gly Thr Ile Val cct ctc ggc ggc tgg 259 Pro Leu Gly Gly Trp tcg gac gcc ctc cgc 307 Ser Asp Ala Leu Arg WO 01/00805 WO 0100805PCTIBOO/00926 ctg gag gtc gcc cca ttc ggc atc gac gtt gtt gtc atc gaa ccq ggc 355 Leu Giu Val Ala Pro Phe Gly Ile Asp Val Val Val Ile Giu Pro Gly 75 80 ggc atc gcc acc gag tgg gga gga atc gct gcc gac aat ctc gac gca 403 Gly Ile Ala Thr Giu Trp Gly Gly Ile Ala Ala Asp Asn Leu Asp Ala 95 100 gtg tcg aaa gac agc gca tac aag cgc cag gct gac gca gta tcg aag 451 Val Ser Lys Asp Ser Ala Tyr Lys Arg Gin Ala Asp Ala Val Ser Lys 105 110 115 tcg ttg cga tct gag gcg aac agc aac cgc aac tca cca ccg tcg gtt 499 Ser Leu Arg Ser Giu Ala Asn Ser Asn Arg Asn Ser Pro Pro Ser Val 120 125 130 gtc gcc gat gcg att gga aag gcc gtg acg gca cgt cac ccc aag acc 547 Vai Ala Asp Ala Ilie Gly Lys Ala Val Thr Ala Arg His Pro Lys Thr 135 140 145 cgc tat gcc atc gqc ttc ggt gcc aaa ccg ctg att gcc tcg cgc aac 595 Arg Tyr Ala Ile Gly Phe Gly Ala Lys Pro Leu Ile Ala Ser Arg Asn 150 155 160 165 atc ctc acc gat cgc cag ttc gac cca gtq atc act cga gcq act ggc 643 Ile Leu Thr Asp Arg Gin Phe Asp Pro Val Ile Thr Arg Ala Thr Gly 170 175 180 gtc ccc cgc gac tgaccactct tctgcgcccg tca 678 Val Pro Arg Asp 185 <210> 112 <211> 185 <212> PRT <213> Corynebacterium giutarnicum <400> 112 Met Thr Ilie Asp Glu Gly Arg Arg Gin Phe Glu Val Asn Val Phe Gly 1 5 10 Ala Met Ala Leu Thr Arg Leu Val Leu Pro His Met Gin Lys Gin Lys 25 Trp Gly Thr Ile Val Asn Ile Thr Ser Met Gly Gly Lys Ile Tyr Thr 40 Pro Leu Gly Gly Trp Tyr His Gly Thr Lys Phe Ala Leu Giu Ala Leu 55 Ser Asp Ala Leu Arg Leu Glu Val Ala Pro Phe Gly Ile Asp Val Val 70 75 Val Ile Giu Pro Gly Giy Ile Ala Thr Giu Trp Gly Gly Ile Aia Ala 90 Asp Asn Leu Asp Ala Val Ser Lys Asp Ser Ala Tyr Lys Arg Gin Ala 100 105 110 Asp Se r Arg 145 Ile Thr WO 01/00805 Ala Val SE 115 Pro Pro Se 130 His Pro L5, Ala Ser Ax Arg Ala Tt 18 Is -9 Lys Ser Leu Arg 120 Val Val Ala Asp 135 Thr Arg Tyr Ala 150 Asn Ile Leu Thr 165 Gly Val Pro Arg PCTIBOOOO926 Ser Glu Ala Asn Ser Asn Arq Asn 125 Ala Ile Gly Lys Ala Val Thr Ala 140 Ile Gly Phe Gly Ala Lys Pro Leu 155 160 Asp Arg Gin Phe Asp Pro Val Ile 170 175 Asp 185 <210> 113 <211> 406 <212> DNA <213> Corynebacteriun giutanicum <220> <221> CDS <222> (101)..(406) <223> FRXA02836 <400> 113 ccatcactcq gcttCaCcgt ctaCggt9Ct gcccgcaqqa gCttCagacg ggatccaccc cctcgagatg gacgtcaccg 99C Gly cga Arg aaC Asn tat Tyr ctg Leu 99 C Gly gtg ValI cag Gln Ctg Leu tcg Ser acc Thr 9CC Ala acc Thr ttc Phe

CCC

Pro atg Met aag Lys CCa Pro gag Giu gtc Val a tg Met 999 Gly gCC Ala ggC Gly gga Gly aat Asn Cag Gin aag Lys ctc Le u atc Ile gga Gly ttC ggC Phe Gly 15.

Caa aag Gin Lys tac acg Tyr Thr gCC CtC Ala Leu gtt gtt Val Val 80 gCt gCC Ala Ala 95 CagagCgCCC CCaaaagCtC atg aca atC gat gaa Met Thr Ilie Asp Giu 1 gCg atg 9CC CtC aCC Ala Met Ala Leu Thr tgg g99 acg atC gtg Trp Gly Thr Ile Vai CCt CtC 99C 99C tg Pro Leu Gly Gly Trp tC9 gac 9CC CtC C9C Ser Asp Ala Leu Arg gtc atC gaa CC9 99C Val Ile Giu Pro Gly gaC aat CtC gac gca Asp Asn Leu Asp Aia 100 115 163 211 259 307 355 403 WO 01/00805 <210> 114 <211> 102 <212> PRT <213> Corynebacterium giutamicum <400> 114 Met Thr Ile Asp Giu Gly Arg Arg 1 5 Ala Met Ala Leu Thr Arg Leu Val 1 Trp Gly Thr Ile Val Asn Ile Thr! Pro Leu Gly Gly Trp Tyr His Gly Ser Asp Ala Leu Arg Leu Giu Val 1 70 Val Ile Giu Pro Gly Gly Ile Ala Asp Asn Leu Asp Ala Val 100 PCTIBOO/00926 Glu His Gi y Ph e Phe 75 Trp Val Lys Ile Gi u Asp Ile Phe Gly Gin .Lys Tyr Thr Ala Leu Val Val Ala Ala <210> 115 <211> 1227 (212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (1204) <223> RXA02578 <400> 115 ggcaaaaatg aggaacagca cgcccgcaat aatgaggacc aacagcccac acctttccgc taaactcgca tgttgaaata gca Al a gca Ala tcc Ser ttt Phe cgc Arg tcc Se r ccc Pro gCC Al a gttgcagatc gcttcataaa atg tct acc caa tca Met Ser Thr Gin Ser 1 tca ctc gag gga cta Ser Leu Giu Gly Leu cac gtt gca ttc caa His Val Ala Phe Gin gta ctc gcg cgt ttc Val Leu Ala Arq Phe gcc ttc gtt ctt tgg Ala Phe Val Leu Trp WO 01/00805 WO 0100805PCTIBOOIOO926 cga cgc Arg Arg gCC cqc Ala Arg ttt att Phe Ilie tac tgg Tyr Trp tgc gtg Cys Val 135 ttg gat Leu Asp 150 gca gtg Ala Val ttg gac Leu Asp tgc t 9 9 Cys Trp ttc cca Phe Pro 215 gct tgg Ala Trp 230 CCC agc Pro Ser gct gta Ala Val ctt gat Leu Asp ttc ctc Phe Leu 295 cgC Arg atc Ile

CCC

Pro cca Pro 120 gaa Gi u agg Ar9 t tg Leu gag Glu ttt Phe 200 cgg Arg atc Ile

CCC

Pro ttt Phe t cc Se r 280 tgg Trp 9cc ggg Ala Gly atg ccc Met Pro acc ggc Thr Gly 105 gac ggg Asp Gly gtg gcg Val Ala ttt ggt Phe Gly agt ct9 Ser Leu 170 ggg tgg Gly Trp 185 gca gtc Ala Val gtt ccg Val Pro gcg ggC Ala Gly tgg gaa Trp Giu 250 ctg gtg Leu Vai 265 agt tgg Ser Trp cac ctt His Leu caa cca gtg gga Gin Pro Val Gly Ctg tac tac ctc aaa cgc cta Leo Tyr Tyr Leu Lys Arg 75 gca Al a ccc Pro ctc Leu ttt Phe cg g Arg 155 gcq Ala gcg Ala 99C Gly agc Se r caa Gin 235 ttc Phe gtt Val atg Met ccc Pro tac Tyr tgg Trp atg Met tac Tyr 140 C C9 Pro t 99 T rp aac Asn a tq Met ttt Phe 220 gaa Glu aac As n ccc Pro aaa Lys gtg Val1 300 t 99 Trp tta Leo a ca Thr 125 ctg Leu qtg Val c9 Pro atg Met atc Ile 205 gtg Val1 t 99 Trp tta Leu tac T yr a cg Th r 285 C tg Leu att Ile a cg Thr cac His ctt Leo 145 ttg Leu ctt Leo cca Pro att Ile tta Leo 225 cta Le u 9C9 Al a a cg Thr tgg Trp ttc Phe 305 gca Al a atg Met ctt Leo 130 ctc Leo att Ile gtg ValI

CCC

Pro gaa Glu 210 gCt Ala gt Gly 99c Gly ccc Pro t c9 Se r 290 cca Pro gtc ValI a cc Thr 115 tgg Trp gcg Ala gtt Val gag Glu gct Ala 195 gga Gly tta Leu t ta Leu aca Thr tct Se r 275 tat Tyr ctg Leu ctg Leu 100 caa Gin tCC Ser t 99 Trp gg t Gly cat His 180 tac Tyr 9t t Val1 gtg Val1 gtg Val1 ctt Leo 260 cg99 Arg tcc Se r Ct C Leo 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 WO 01/00805 ttg c ct tta ttt agt gga aat ttc Leu Pro Leu Phe Ser Gly Asn Phe 310 315 ttg ttg acg atc cca gtt gcc gcc Leu Leu Thr Ile Pro Val Ala Ala 330 ccc atc agc ggt gga ccc ggc gcg Pro Ile Ser Gly Gly Pro Gly Ala I 345 att cac cat ttt tct ggg ggt agg I Ile His His Phe Ser Gly Gly Arg 360 365 aag <210> 116 <211> 368 <212> PRT <213> Corynebacterium giutamicum PCTIiBOO/00926 gtg ttc atc gtg acg gtc 1075 Val Phe Ile Val Thr Val 320 325 tac acc ttc atc gaa gag 1123 Tyr Thr Phe Ile Glu Glu 340 agg ctg ggg gtc gtt agg 1171 Arg Leu Gly Val Val Arg 355 aaa tgatgaattg gcaccacgtc 1224 Lys 1227 <400> 116 Met Ser Thr Gin Ser Tyr Ala Pro Ile Arg His Arg Gly Phe Ile Ser Leu Val Le u Phe Leu Aila Met Leu 130 Le u Ile Vali Glu Aila Aia Val1 Lys Vali Thr 115 Trp Ala Vali G lu Gi y Ph e Arg Le u Arg be u 100 Gin Ser Trp Gly His 180 Ile Val Phe Arg Ile Pro Pro 120 Glu Arg Leu Glu Al a Asp Val1 Ala Met Th r 105 Asp Val1 Phe Se r Gly 185 Leu Ala Val Gin Ala Pro Leo Phe Arg 155 Ala Al a Gl y Ser Phe Pro Tyr T rp Met T yr 140 Pro T rp Asn Al a Gi y Leo Le u Thr Asn Leu Met Ile Ile 175 Ile WO 01/00805 PTIO/02 PCT/IIBOO/00926 Pro Ile Leu 225 Le u Ala Thr Trp Phe 305 Phe T hr Le u Pro Glu 210 Al a Gi y Gly Pro Se r 290 Pro Ile Phe Gly Ala 195 Gly Le u Leu Thr Se r 275 Tyr Le u Va 1 Ile Val 355 Tyr Val1 Val Val1 Leu 260 Arg Se r Leu Thr Glu 340 ValI Ala Cys Trp Phe Ala Val Gly Met Ile Ala Ala Glu 200 205 Arg Val His 245 Phe Leu Ile Gly Val1 325 Glu Arg Phe Ala 230 Pro Ala Leu Phe Leu 310 Leu Pro Ile Val Ala Trp Le u 265 Ser His Phe Ile Gly 345 Phe Pro Gl y Glu 250 Val Trp Le u Se r Pro 330 Gly Se r Se r Gin 235 Phe Val Met Pro Gly 315 Val1 Pro Gly Phe 220 Glu Asn Pro Lys Val1 300 As n Ala Gly Gly Gly Pro 240 Le u Gly Thr Val1 Val1 320 Tyr Arg Lys <210> 117 <211> 924 <212> DNA <213> Corynebacterium qlutamicum <220> <221> CDS <222> (101)..(901) <223> RXA02150 <400> 117 gtqtttttcg gtgggctgcg atgacgcatg tccaccaaaa gaqccacccc ttaaagaaat taaaaaqtgg ttttqgtagc ttcgcagcaa aatacacatc gtg ggt aac gta ttc Val Gly Asn Val Phe 1 tta gaa gtt cct aca gca gta aag cgc gaa gaa ggg gta aac cca aac Leu Glu Val Pro Thr Ala Val Lys Arg Glu Glu Gly Val Asn Pro Asn 15 atc atg aaa aac aac tgg tat cgg ctt ttc aag tat qtg cta att qgc Ile Met Lys Asn Asn Trp Tyr Arg Leu Phe Lys Tyr Val Leu Ile Gly 30 WO 01/00805 TJBO/02 PCT[IBOO/00926 ccg Pro atc Ile atg Met cca Pro ca g Gin gca Ala gat Asp gat Asp 150 gaa Glu g cg Ala aa g Lys ttg Leu atg Met 230 tct Ser ttg Leu gca Al a t cc Se r aag Lys t gq Trp aat As n 120 gga Gly cgc Arg ggt Gly cct Pro gga Gly 200 cct Pro att Ile ga t Asp Cqt gtg tac Arg Val Tyr gaa ggt gcc Glu Gly Ala ttt tat ttt Phe Tyr Phe 75 gcc gaa tac Ala Glu Tyr ttt ttt act Phe Phe Thr 105 gcc atq gat Ala Met Asp gac ctc ttc Asp Leu Phe atc tac aaq Ile Tyr Lys 155 acg aca gtt Thr Thr Val 170 atc gga agt Ile Gly Ser 185 agc cca att Ser Pro Ile gga acc tac Gly Thr Tyr ctt gct gat Leu Ala Asp 235 gtg aaa aac Val Lys Asn 250 cct tca cag Pro Ser Gin 265 aac cgc ccg gag atc gaa ggc aaa Asn Arg Pro Giu Ile Giu Gly Lys gaa aac Glu Asn 45 gcg atc atg Ala Ile Met Ccc ctg ctg Pro Leu Leu ttc aca tca Phe Thr Ser tct gtg gg Ser Val Gly 110 tct ttg atg Ser Leu Met 125 ggt att tac Gly Ile Tyr 140 ggc aaa acc Gly Lys Thr atc ccc gtt Ile Pro Val tgg ttt ccg Trp Phe Pro 190 gat ccc ctc Asp Pro Leu 205 gaa gca qcg Giu Ala Ala 220 Ctc act ggt Leu Thr Gly gct ctg gag Ala Leu Giu gcg tcc aac cac Ala Ser Asn His tqc cca cgg cag Cys Pro Arg Gin 80 cca ggt att aaa Pro Gly Ile Lys 95 caa gta ccc ctg Gin Val Pro Leu aat acc gCC aaa Asn Thr Ala Lys 130 cct gaa gga tct Pro Giu Gly Ser 145 gga atg gcc tat Gly Met Aia Tyr 160 gcc-atg att ggc Ala Met Ile Giy 175 aaa ccc gca aaa Lys Pro Ala Lys gca ttc gtc aaa Ala Phe Val Lys 210 cgc aag ctg aca Arg Lys Leu Thr 225 cag ccg tat gtt Gin Pro Tyr Val 240 gaa gga aaa gga Glu Gly Lys Gly 255 ga a Glu ctg Leu 9gc Gly ga c Asp 115 atg Met cg t Arg qt t Val1 agc Se r gtc Val 195 ga a Giu gat Asp ga t Asp tac T yr gca Al a acc Thr a ag Lys 100 cgc Arg gtg Val1 tcg Se r gcg Al a cg g Arg 180 agg Arg cat His cac His 9 C9 Ala C cg Pro 260 gtg Val1 ttc Phe atg Met acc Thr ctg Leu ccc Pro atg Met 165 gac Asp atc Ile ggg Gly gtt Val1 tac Tyr 245 gag Glu ggc aca gct Gly Thr Ala taatcgggtc ttttctgtta aaa WO 01/00805 'z210> 118 <211> 267 <212> PRT <213> Corynebacterium glutamicum <400> 118 Val Gly Asn Val Phe Leu Giu Val Pro PCTJ[BOO/00926 1 Gly T yr Glu Asn Arg Ile Pro Ala Gly 145 Al a Ile Ala Val Leu 225 Tyr Lys Ile Pro Ile Met 70 Pro Gin Ala Asp Asp 150 Glu Ala Lys Le u Met 230 Se r GI y Met Phe Pro Asp Ala Lys Asp Arg 135 Gly Thr Asn Val1 Lys 215 Phe Lys Th r Lys Le u Al a Ser Lys Trp Asn 120 Gly Arg Gly Pro Gly 200 Pro Ile Asp Ala Asn Arg Glu Phe Ala Phe 105 Ala Asp Ile Thr Ile 185 Se r Gly Le u Val Pro 265 Ala Trp Tyr Ala Phe 75 Tyr Thr Asp Phe Lys 155 Val1 Ser Ile Ty r Asp 235 As n Gin Val Tyr Asn Ala Pro Phe Ser Ser Gly 140 Gly Ile Trp Asp Glu 220 Leu Ala Lys Arg Arg Ile Le u Thr Val1 Le u 125 Ile Lys Pro Phe Pro 205 Ala Thr Leu Arg Le u Pro Met Le u Ser Gly 110 Met Tyr Thr Vali Pro 190 Le u Ala Gly Glu Glu Phe Glu Ala Cys Pro Gln As n Pro Gly Ala 175 Lys Ala Arg Gin Glu 255 Glu Ly s Ile Ser Pro Gly Val Thr Glu Met 160 Met Pro Phe Lys Pro 240 Gly <210> 119 <211> 504 WO 01/00805 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(481) <223> RXA00607 <400> 119 cactgccagc aacgccaacg gttgaacccg aaccagaaqq PCT/IBOO/00926 cgatgaagac tggcccgaac ccatcaaccc cgcaggcgat aacaaagagg aggcaaaccg atg att ctc gca ctg Met Ile Leu Ala Leu ata Ile cgc Arg atc Ile ag Ar g ctc Le u t tg Le u gac Asp 105 ctt Leu atc Ile ctg Leu acc T hr a cc Thr qca Ala ga t Asp ttc Ph e gtc Val1 tac Tyr Ccg Pro gcc Ala gca Al a ca a Gin 1 gtc tac ctc Val Tyr Leu 15 tca ctg atc Ser Leu Ile ccc acg tgg Pro Thr Trp qcc gcc gat Ala Ala Asp gcg atg gcc Ala Met Ala agc gac gac Ser Asp Asp 95 act act aqc Thr Thr Ser cag caa Gin Gin cac gca His Ala ggc gaa Gly Glu ctc ccc Leu Pro aca acc Thr Thr cgq tcc Arg Ser 100 cgt tca Arg Ser 115 acc aac cca aca gat cag gag gat aaa gct taaatggcca tggatgttct Thr Asn Pro Thr Asp Gin Giu Asp Lys Ala 120 125 cc t <210> 120 <211> 127 <212> PRT <213> Corynebacterium giutarnicum <400> 120 Met Ile Leu Ala Leu Thr Val Ala Ile Leu Phe Gly Gly Gly Val Tyr 1 5 -10 Leu Ile Gin Gin Arg Gly Met Val Arg Ile Val Phe Gly Met Ser Leu 25 Ile Trp Asp Ala Asp Se r WO 01/00805 Gly His Al Arg Giy Gl Pro Leu Pr Thr Thr Th Thr Arg Se Ala Arq Se 115 a u 0 r r 0 r Ala Ala Gin Ile Ile Val1 As n Phe Al a 70 Met Glu Thr Le u Pro 55 Phe Leu Pro As n T hr 40 Asp Val1 Ala Asp Pro 120 Ala Leu Ile Le u Ser G lu Gi y Thr Val1 Gly Pro Asp 125 PCTIIBOO/00926 Pro Thr Ala Ala Ala Met Ser Asp Thr Thr Ala <210> 121 <211> 1119 <212> DNA <213> Corynebacterium qiutamicum <220> <221> CDS <222> (101)..(1096) <223> RXA02397 <400> 121 qtqtgaataa aacttgtttc ctggtcattt cccctactga tagaaaccag agacaagctt aagaaggaca ggggccggcc a ca Th r cgc Arg acc Thr cag Gin gc t Al a aag Ly s ga t Asp cag Gln caa Gin gat Asp ctg Le u gtg ValI atc Ile att Ile g ca Al a gcg Ala gac Asp 75 agc Ser actgcgctta tqcctatgct atg aat gat ttc gaa Met Asn Asp Phe Glu 1 ccc gca gcc cga agc Pro Ala Ala Arg Ser gca cta ccc aac ttg Ala Leu Pro Asn Leu.

ctt ctt gat gcg gta Leu Leu Asp Ala Val gcg tcg aca agc aat Ala Ser Thr Ser Asn acc agc ctg att qgc Thr Ser Leu Ile Gly tcg ttt ggg ttc caq Ser Phe Gly Phe Gin 100 WO 01/00805 WO 0100805PCT/IBOO/00926 cct tct gaa act Pro att Ile gcg Ala tCt Se r 150 aaa Lys gtg Val gaa Glu aag Lys ca g Gin 230 ctt Le u atc Ile ctt Le u acg Thr tcc Ser 310 Ser Giu Thr 105 ttt tat Cag Phe Tyr Gin 120 ccg cjac ttt Pro Asp Phe 135 gtc acc gaa Vai Thr Giu gcg cag cgt Ala Gin Arg gtg gtq agt Val Val Ser 185 tac acc ccc Tyr Thr Pro 200 aac tgt tgg Asn Cys Trp 215 gtt Ccg gaa Vai Pro Giu ccc cag atg Pro Gin Met caa gaa tcg Gin Giu Ser 265 tcc cca cqc Ser Pro Arg 280 cgt cct ctc Arg Pro Leu 295 cac caa ttg His Gin Leu aca caa tcg Thr Gin Ser gcg Al a cac His cag Gin cca Pro 9CC Ala 170 agg Arg a cc Thr tac Tyr att Ile tgg Trp 250 gaa Giu acc Thr tcc Se r gag Giu cac ccg cta atg atc gct atc cct Pro ga t Asp aat Asn 140 ctt Leu gaa Giu a cq Thr 9gc Gly att Ile 220 cqc Arg aaa Lys gca Ala aac Asn gc t Aia 300 ctg Leu Ile ccg Pro atg Met aaa Lys ggc Giy 175 cg c Arg ccc Pro tct Ser ggc Gly gt a Val1 255 atc Ile tgq Trp t gq Trp gga Ile agc Ser cg c Ar g 145 ttt Phe ctg Le u 99 g Giy ccg Pro acc Thr 225 att Ile gcc Al a atg Met aac Asn gt g Val1 305 aca Pro ga t Asp 130 a gg Arg tcc Se r t 99 Trp t ta Le u ctg Le u 210 att Ile ttc Phe ggc Gly* g cg Aia agc Se r 290 gtg Vali ggg gag Giu 115 ga c Asp cg t Ar g gga Giy aag Lys act Thr 195 atg Met 9t Giy tgt Cy s aaq Lys cg a Arg 275 tat Tyr ct 9 Le u ccg 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1119 Leu Arg Gly Asp Thr Gly Pro 320 taaccaaaag ttcacgtgga gat gca gct qgt Ala Ala Giy 330 WO 01/00805 PCTJIBOO/00926 (210> 122 (211> 332 'z212> PRT <213> Corynebacterium qiutarnicum <400> 122 Met Asn Asp Phe Giu Thr Thr Ile Asp Arg Ile Ser Lys Giu Gin Asp 1 5 10 Pro Ala Ala Arg Ser Arg Val Giu Gin Phe Ile Val Giu Thr Val Arg 25 Ala Leu Pro Asn Leu Thr Thr Lys Gin Giy Ala Ser Leu Ala Ile Gin 40 Leu Leu Asp Ala Val Gin Leu Ala Asp Ala Aia Giy Thr Lys Gly Gly 55 Ala Ser Thr Ser Asn Ala Ser Ser Leu Pro Asp Thr Phe Asp Ala Leu 70 75 Thr Ser Leu Ilie Gly Lys Leu Asp Val Arg Ser Asp Ser Giu Trp Arg 90 Ser Phe Gly Phe Gin Pro Ser Giu Thr Ala His Pro Leu Met Ile Ala 100 105 110 Ilie Pro Giu Ile Giu Ile Phe Tyr Gin His Thr Asp Vai Giu Pro Gly 115 120 125 Ser Asp Asp Ala Val Ala Pro Asp Phe Gin Giu Asn Gin Asp Met Trp 130 135 140 Arg Arg Arq Leu Gly Ser Val Thr Giu Pro Asn Leu Ilie Tyr Lys Giu 145 150 155 160 Phe Ser Gly Pro Giy Lys Ala Gin Arg Ala Val Giu Met Leu Gly Asn 165 170 175 Leu Trp Lys Ilie Gly Val Val Val Ser Arg Asn Thr Giu Ser Arg Leu 180 185 190 Giy Leu Thr Arg Val Giu Tyr Thr Pro Thr Pro Gly Giu Val Pro Val 195 200 205 Pro Leu Met Ser Giu Lys Asn Cys Trp Tyr Ser Ile Arg Val Ser Giu 210 215 220 Thr Ile Gly Giu Asn Gin Val Pro Giu Ile Val Arg Cys Leu Gly Giu 225 230 235 240 Ilie Phe Cys Gly Tyr Leu Pro Gin Met Trp Leu Lys Giu Pro Val Lys 245 250 255 Ala Gly Lys Leu Arg Ilie Gin Glu Ser Giu Ala Ala Ala Tyr Ile Ala 260 265 270 Met Ala Arg Leu Asp Leu Ser Pro Arg Thr Gly Asn Thr Thr Trp Thr 275 280 285 Asn Ser Tyr Ilie Ser Thr Arg Pro Leu Ser Pro Ala Phe Arg Trp.Asp WO 01/00805 PCT/IBOO/00926 290 295 300 Val Leu Giu Ala Ser His Gin Leu Glu Asn Leu Leu Arg Gly Asp 310 315 320 Gly Pro Val Thr Ala Thr Gin Ser Ala Ala Gly 325 330 <210> 123 <211> 1462 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <z222> (1462) <223> RXN03110 <400> 123 aatgagtgaa cctgcgcttg aactataccc cctggggtat ccgaggggta tctaqttttt acgttgagaa ggagagctca tactgtagat tctcaatacc gac Asp aac Asn ga c Asp cat, His act Thr tat Tyr gga Gly cac His 120 ttc Phe ga c Asp gc a Ala gcc Al a cat His gcc Ala ttc Phe ctg Le u tcg Ser ctc Leu gcg Ala gtg ValI aaa Lys gac Asp atg Met gcc Ala ctc LeU ctg Le u gg C Gly 140 cag Gln gtt Val1 aac As n 45 tat T yr tac T yr gag Glu aca Th r ctg Le u 125 ga t Asp gcc Ala gat Asp ga Gly ttg Le u gtc Val1 cga Arg gct Ala 110 aag Lys ttc Phe ag c Ser 15 ccg pro atg Met tca Ser t ca Ser ata Ile 95 gt t Val gac Asp gtt ValI tat Tyr cgt Arg gag Giu gga Gly ggg Gly tgc Cys ca c His ggc Gly ttc Ph e gtg ctt att gaa cgc Val Leu Ile Glu Arg 1 ttc att ggc tgc caa Phe Ile Gly Cys Gln cgc gat att gcc gtc Arg Asp Ile Ala Val att gtt gga gtt acc Ile Val Gly Val Thr acc cgt gag tta gct Thr Arg Giu Leu Ala gag ggc ggc gcc gat Giu Gly Gly Ala Asp gac ggc agc gag att Asp Gly Ser Glu Ile 100 acc cca ggc cat acc Thr Pro Gly His Thr 115 gcg ttc gca gat gag Ala Phe Ala Asp Glu 130 gcg ggt gat ctt ggc Ala Gly Asp Leu Gly 145 WO 01/00805 PT10102 PCT[IBOO/00926 cga Arg 150 gag Giu tig Leu igi Cys cg i Arg gg a Gly 230 ttc: Phe gct Al a att Ilie gc t Al a t cg Se r 310 gcg Al a qtg Val1 gac Asp gaa Giu cca Pro ggg Gly cci Pro ggc Gi y caa Gin 215 tic Phe 9gc Gi y cgc Arg gt Val ggt Gly 295 igg Trp gca Ala gga Gly cta Le u tac Tyr 375 ga t Asp gc t Ala ga t Asp aaa Lys 200 iii Phe at Ile agg Arg gag GI u gt Vai 280 gca Al a a cc Thr a gc Ser atc Ile gt Val 360 gati Asp ttg ctc gat Leu Leu Asp 155 cgc caa atg Arg Gin Met 170 cac aic cag His Ilie Gin 185 gcc tig ggt Ala Leu Giy gcg igg tgg Ala Trp Trp gat gag cti Asp Glu Leu 235 atg aag agg Met Lys Arg 250 ctg tig cca Leu Leu Pro 265 gat acc cgc Asp Thr Arg gtg aai att Val Asn Ile git gat ccc Val Asp Pro 315 caa ait ggt Gin Ile Giy 330 gat aat gtt Asp Asn Val 345 gca ccg caa Ala Pro Gin cta cii ct Leu Leu Leu gaa gca gct ggg gga gtg gac act cgt ttt Glu Ala Ala Giy Gly Val Asp Thr Arg Phe 160 165 tic Phe atc Ile tcg Ser gg a Giy 220 cig Leu caa Gin ca g Gin tca Ser cct Pro 300 gag Giu gec Ala gct Al a act Thr gati Asp 380 aag Lys cat His aca Thr gag Giu cci Pro 240 ggg Gly ici Ser gii ValI i cg Se r cga Arg 320 tgg Trp acc Thr ga t Asp cqc Arg ga a Glu gg t Giy aca Thr g ca Ala 225 gati Asp ccc Pro gati Asp cac His aig Met 305 gC t Ala gac Asp aac Asn ca g Gin ag i Se r 385 aaa Lys icc Se r cii Le u 210 gat Asp gca Ala gca Al a ctg Leu cag Gin 290 g cg Ala i ig Leu cac His ttt Phe cig Le u 370 gaa Giu tic Phe gg i Gly 195 gga Gi y gati Asp cci Pro ati Ile cac His 275 ggc Gly aaa Lys gt t Val1 a ig Met gati Asp 355 ga t Asp gic Val1 cig Leu 180 icc Ser tat Tyr gaa Giu gca Al a a ig Met 260 gac Asp aci Thr iii Phe cig Le u git Val 340 ggg Gly ga a Giu gaa Giu aca Thr g cg Ala ga a Giu ca a Gin iac Tyr 245 ggc Giy gic Val1 gia Val ggc Gly cic Leu 325 cg c Arg gig Val tg Leu gaa Giu 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 WO 01/00805 ggc tac atc cc Giy Tyr Ile Pi 390 aat ctg gaq ae Asn Leu Giu L agt gga aca cc Ser Gly Thr Al 42 ttt gat gtg gt Phe Asp Val Va 440 aac Asn ctc Leu cg t Arg atc Ile caa Gin ct g Leu tgC Cys 420 gct Aia Arg PCTIBOO/00926 tgg 1315 Trp 405 aag i363 Lys 9gt 1411 Giy cac 1459 His 1462 <210> i24 <211> 454 <2i2> PRT <213> Corynebacterium giutamicum <400> 124 Val Leu Ile Giu 1 Phe Arg Ile Thr Giu Asp Thr Aia Aia 145 Vai Giu Giy Ile Gly Giu Gly Ser Giy 115 Ala Asp Thr Ph e Cys Ala ValI Le u Ala Giu 100 His Asp Leu Arg Leu Arg 5 Gin Val Thr Ala Asp Ile Thr Giu Gi y Phe 165 Th r Ile Tyr Asp Glu Ala Tyr Giu Ala 70 Trp Arg Pro Pro Ar g 150 Giu Leu His Le u Th r Al a Gin Le u Giu Gly 135 Pro Gly Pro Asp 10 Ala Ala His Ala Phe 90 Leu Ser Leu Leu Gin 170 Ile Leu Ala Gin Ala Ser Tyr Val1 Lys Ala Thr Asp Vai Phe Thr Asp 155 Met Gin ValI Ly s Asp Met Ala Leu Leu Gly 140 Glu Phe Ile Val1 As n Tyr Tyr G iu Th r Le u 125 Asp Ala Lys Phe Asp Gly Leu Val Arg Al a 110 Lys Phe Al a Se r Pro Pro Met Se r Ser Ile Val1 Asp Val Gly Le u 175 Gly WO 01/00805 PCT/IB00/00926 180 185 190 Gly Ser Gly Ser Ala Cys Gly Lys Ala Leu Gly Ser Val Pro Ser Thr 195 200 205 Thr Leu Gly Tyr Glu Arg Gin Phe Ala Trp Trp Gly Lys Tyr Leu Glu 210 215 220 Ala Asp Asp Glu Gin Gly Phe Ile Asp Glu Leu Leu Glu Gly Gin Pro 225 230 235 240 Asp Ala Pro Ala Tyr Phe Gly Arg Met Lys Arg Gin Asn Arg Gin Gly 245 250 255 Pro Ala Ile Met Gly Ala Arg Glu Leu Leu Pro Gin Leu Glu Ala Ser 260 265 270 Asp Leu His Asp Val Ile Val Val Asp Thr Arg Ser Ala Asp Glu Val 275 280 285 His Gin Gly Thr Val Ala Gly Ala Val Asn Ile Pro Ala Gly Asn Ser 290 295 300 Met Ala Lys Phe Gly Ser Trp Thr Val Asp Pro Glu Lys Asp Ser Arg 305 310 315 320 Ala Leu Val Leu Leu Ala Ala Ser Gin Ile Gly Ala Met Glu Met Trp 325 330 335 Asp His Met Val Arg Val Gly Ile Asp Asn Val Ala Gly Phe Ile Thr 340 345 350 Asn Phe Asp Gly Val Asp Leu Val Ala Pro Gin Thr Val Ser Pro Asp 355 360 365 Gin Leu Asp Glu Leu Glu Tyr Asp Leu Leu Leu Asp Val Arg Asn Arg 370 375 380 Ser Glu Val Glu Glu Gly Tyr Ile Pro Gly Ala Leu His Ile Asn Gly 385 390 395 400 Ala Ser Val Leu Trp Asn Leu Glu Lys Leu Pro Arg Asp Gly Lys Ile 405 410 415 Val Ser Tyr Cys Lys Ser Gly Thr Arg Ser Ser Ile Ala Ala Ser Thr 420 425 430 Leu Arg Asn Ala Gly Phe Asp Val Val Glu Leu Gin Gly Ser Tyr Asp 435 440 445 Asn Trp Val Arg His Asn 450 <210> 125 <211> 1023 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS WO 01/00805 <222> (1)..(1023) <223> FRXA00660 PCTJIBOO/00926 <400> 125 cca ggc cat Pro Gly His 1 ttc gca cat Phe Ala His ggt gat ctt Gly Asp Leu gac act cqt Asp Thr Arg aaa ttc ctg Lys Phe Leu tcc ggt tcc Ser Gly Ser ctt gga tat Leu Gly Tyr gat qat gaa Asp Asp Glu 115 gca cct gca Ala Pro Ala 130 qca att atg Ala Ile Met 145 ctg cac gac Leu His Asp cag ggc act Gin Gly Thr gcg aaa ttt Ala Lys Phe 195 ttg gtt ctg Leu Val Leu 210 cac atg gtt ccg gaa Pro Glu 5 cca gga Pro Gly cga cca Arg Pro gag ggg Glu Gly ttg cct Leu Pro 70 tgt ggc Cys Gly cgt caa Arg Gin qga ttc Gly Phe ttc ggc Phe Gly gct cqc Ala Arg 150 att gtt Ile Val 165 gct ggt Ala Gly tcg tgg Ser Trp gcg gca Ala Ala gtq gga cac tta tcg ttc Ctg ctg aag His Leu Ser Phe Leu Leu Lys gac ggc gcg Asp Gly Ala ttc Phe gat Asp gc t Ala gat Asp aaa Lys ttt Phe att Ile agg Arg 135 gag Glu gtt Val1 gca Al a a cc Thr agc Ser 215 atc a tg Met ttg Leu 40 cgc Arg cac His gcc Ala gcg Ala qat Asp 120 atg Met ctq Le u gat Asp gtg Val1 gtt Val1 200 caa Gin gat ctc Le u ctc Le u caa Gin atc Ile t tg Le u t gg T rp 105 gag Glu a ag Lys5 t tg Le u acc Thr aat As n 185 ga t Asp att Ile aat act Thr gat Asp a tg Met ca g Gin ggt Gly 90 tqq Trp ctt Le u agg Arg cca Pro cg c Arg 170 att Ile ccc Pro ggt Gly gt t ttc Phe gct Ala agc Ser cct Pro cct Pro tat Tyr ggc Gly 125 agg Arg gaa Glu gat Asp ggc Gly gat Asp 205 gag Giu ttt gt t Val1 ggg Gi y t tg Leu ggt Gly t ca Ser ctg Leu 110 caa Gin caa Gin gct Al a gaa Glu aat Asn 190 t cc Ser atg Met atc ttc Phe gga Gi y aag Lys cat His aca Thr gag Giu cc t Pro ggg Gly tct Ser gtt Val1 175 t cg Ser cg a Arg tgg Trp acc gcg Ala gt g Val gaa Glu ggt Gly a ca Th r g ca Al a ga t Asp ccc Pro qa t Asp 160 cac His atg Met gct Ala ga c Asp aac 48 96 144 192 240 288 336 384 432 480 528 576 624 672 720 WO 01/00805 His Met Val Arg Val Gly Ile Asp Asn 225 230 ttt gat ggg gtg gac cta gtt gca ccg Phe Asp Gly Val Asp Leu Val Ala Pro 245 ctg qat gaa ttg gaa tac gat cta ctt Leu Asp Glu Leu Giu Tyr Asp Leu Leu 260 265 gaa gtc qaa gaa ggc tac atc cca gga Giu Val Glu Glu Gly Tyr Ile Pro Gly 275 280 tcc gtg ctg tgg aat ctg gag aaa ctg Ser Val Leu Trp Asn Leu Giu Lys Leu 290 295 agc tac tgc aag agt gga aca cqc agc Ser Tyr Cys Lys Ser Giy Thr Arg Ser 305 310 cqt aat gct ggt ttt gat gtg gtg gaa Arg Asn Ala Gly Phe Asp Val Val Giu 325 tgg gtc cgg cac aac Trp Vai Arg His Asn 340 <210> 126 <211> 341 <212> PRT <213> Corynebacterium giutamicum <400> 126 Pro Gly His Thr Pro Giu His Leu Ser 1 5 Phe Ala His Giu Pro Gly Phe Met Leu Gly Asp Leu Gly Arg Pro Asp Leu Leu Asp Thr Arg Phe Glu Giy Ala Arg Gin Lys Phe Leu Thr Leu Pro Asp His Ile 70 Ser Gly Ser Ala Cys Gly Lys Ala Leu Leu Gly Tyr Glu Arg Gin Phe Ala Trp 100 105 Asp Asp Giu Gin Gly Phe Ilie Asp Giu 115 120 Thr ga t Asp 255 cgc Arg ggt Gi y atc Ile acc Th r ga c Asp 335 PCTIBOOIOO926 Asn 240 cag 768 Gin agt 816 Ser gca 864 Ala gtg 912 Val ctq 960 Leu 320 aac 1008 As n 1023 Phe 10 Thr Asp Met Gin Gly 90 T rp Leu Gly Phe Gly Lys His Th r Giu Pro Ala Ala Val1 G lu Gly Thr Ala Asp WO 01/00805 PCTJIBOO/00926 Ala Pro Ala Tyr Phe Gly Arg Met Lys Arg Gin Asn Arg Gin Gly Pro 130 135 140 Ala Ilie Met Gly Ala Arg Giu Leu Leu Pro Gin Leu Giu Ala Ser Asp 145 150 155 160 Leu His Asp Val Ile Val Val Asp Thr Arg Ser Ala Asp Giu Val His 165 170 175 Gin Gly Thr Val Ala Gly Ala Val Asn Ile Pro Ala Gly Asn Ser Met 180 185 190 Ala Lys Phe Gly Ser Trp Thr Val Asp Pro Giu Lys Asp Ser Arg Ala 195 200 205 Leu Val Leu Leu Ala Ala Ser Gin Ile Gly Ala Met Giu Met Trp Asp 210 215 220 His Met Val Arg Val Gly Ilie Asp Asn Val Ala Gly Phe Ile Thr Asn 225 230 235 240 Phe Asp Gly Val Asp Leu Val Aia Pro Gin Thr Val Ser Pro Asp Gin 245 250 255 Leu Asp Giu Leu Giu Tyr Asp Leu Leu Leu Asp Val Arg Asn Arg Ser 260 265 270 Giu Val Giu Giu Gly Tyr Ile Pro Gly Ala Leu His Ile Asn Giy Ala 275 280 285 Ser Val Leu Trp Asn Leu Giu Lys Leu Pro Arg Asp Gly Lys Ile Val 290 295 300 Ser Tyr Cys Lys Ser Gly Thr Arg Ser Ser Ile Ala Ala Ser Thr Leu 305 310 315 320 Arg Asn Ala Gly Phe Asp Val Val Giu Leu Gin Gly Ser Tyr Asp Asn 325 330 335 Trp Val Arg His Asn 340 <210> 127 <211> 756 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (733) <223> RXA00801 <400> 127 gqataagttt qtttctgagc gtattggtct tgatqatgtt gaagaggctt tcaacaccat gaagqctgqc gacgtgctgc gttctgtggt ggagatctaa atg gct cac gac gga 115 Met Ala His Asp Gly 1 WO 01/00805 WO 0100805PCT/IBOO/00926 ttg cgc gta gaa aac att gte ace tca Le u Gly gag Giu get Ala aat Asn eca Pro gga Gly get Ala tee Se r aet Thr 150 ttt Phe geg Ala get Arg gaa G lu gtt Val1 gte Val1 gac Asp ate Ile aae As n gga Gly age Ser 135 ttg Le u gac Asp gaa Giu gag Val1 tgg Trp ttc Phe gg t Gly eae His ttc Phe ctg Leu act Thr 120 tge Cys ttc Phe ace Thr ace Thr get Glu gaa Giu ate Ile gga Gly ate Ile gtg Val ace Th r 105 gaa Giu tta Phe eag Gin ate Ile ace Thr 185 cca As n gte Val ate Ile eg t Arg act Th r eac His ea e His etg Leu tae Tyr gg t Giy att Ile 170 gtg Vai cac Ile gac Asp ga t Asp get Ala gte Val1 75 eca Pro gag Glu ate Ile ct e Le u ggg Giy 155 gag Giu eg e Arg ttg Val1 aac As n g eg Ala gtg Val1 60 gea Aia ggt Giy ga t Asp gtg Val1 ect Pro 140 e eg Pro tee Ser act Thr gag Glu Thr aac As n gca Ala 45 aag Lys eca Pro ga e Asp ttg Leu ett Leu 125 gaa Gi u gga Gi y etc Leu ggC Gi y gaa Glu Ser ate Ile cac His gge Gly gag Giu eaa Gin gea Ala 110 aat As n gea Aia g ca Al a aag Lys cat His 190 tgg Trp ggc Gi y 15 t g T rp act Thr att Ile eta Leu a tg Met 95 ga t Asp ace Thr aac Asn act Thr ace Thr 175 ggt G ly att Ile ate Ile gt t Val1 gea Al a ttg Leu tee Se r 80 et g Le u ea g Gin cet Pro gag Giu Gi y 160 tea Se r ga t Asp aaa Lys ttt Phe gtg Val1 g ca Ala tge Cys aag Lys t gg Trp ca g Gin gga Gly etc Leu 145 eg t Arg att Ile eae His ege Arg gee Ala gga Gly ccc Pro ace Thr ga a Giu gag Giu aag Lys eae His 130 tte Phe aag Lys ttg Le u ace Th r ggg Gly 210 gat Asp ga t Asp ate Ile gca Ala ga t Asp ace Thr 100 eaa Gin cet Pro gga Gly age Ser eta Leu 180 gtg Val gg t Gly gat Asp gag Glu cac His gea Ala eac His ate Ile gga Gly ga e Asp tee Se r 165 eca Pro g gg Gly 163 211 259 307 355 403 451 499 547 595 643 691 Ala Glu Ala Pro His Leu 200 taageeeega aegattagta ggc <210> 128 <211> 211 <212> PRT <213> Corynebacterium glutamicun <400> 128 756 WO 01/00805 WO 0100805PCTJIBOO/00926 Met Ala His Asp Gly Leu Arg Val Glu Asn Ile Val Thr Ser Gly Ile 1 5 10 Phe Ala Leu Asp Gly Gly Giu Trp Giu Val Asp Asn Asn Ile Trp Val 25 Val Gly Asn Asp Asp Glu Val Phe Ile Ile Asp Ala Ala His Thr Ala 40 Ala Pro Ile Ile Glu Ala Val Gly Gly Arg Ala Val Lys Gly Ile Leu 55 Cys Thr His Ala His Asn Asp His Ile Thr Val Ala Pro Glu Leu Ser 70 75 Lys Giu Phe Asp Ala Pro Ile Phe Val His Pro Giy Asp Gin Met Leu 90 Trp Glu Giu Thr His Gly Asn Leu Thr His Giu Asp Leu Ala Asp Gin 100 105 110 Gin Lys Phe Gin Ile Ala Gly Thr Giu Leu Ile Vai Leu Asn Thr Pro 115 120 125 Gly His Ser Pro Gly Ser Ser Cys Phe Tyr Leu Pro Giu*Ala Asn Giu 130 135 140 Leu Phe Ser Gly Asp Thr Leu Phe Gin Gly Gly Pro Gly Ala Thr Gly 145 150 155 160 Arg Lys Tyr Ser Ser Phe Asp Thr Ile Ile Giu Ser Leu Lys Thr Ser 165 170 175 Ile Leu Asp Leu Pro Ala Giu Thr Thr Val Arg Thr Gly His Gly Asp 180 185 190 His Thr Ser Val Gly Ala Giu Ala Pro His Leu Giu Giu Trp Ile Lys 195 200 205 Arg Gly His 210 <210> 129 <211> 966 <212> DNA <213>' Corynebacterium giutamicum <220> <221> CDS <223> RXA00821 <400> 129 ttaaaagctt gcttctcgac gcaaaaaccc atccggcgca tcccttcaat gttaggggtg cgctgctatt tttctcccca gttctacgaa atgacttatt gtg act aag ctt gag 115 Val Thr Lys Leu Giu 1 cgc atg gag cat cct gct tac agc caa ttg cgg ccg gtt acc ccg tcc 163 WO 01/00805 WO 0100805PCTIBOO/00926 Arg Met Giu His Pro Ala Tyr Ser Gin Leu Arg Pro Vai Thr Pro Ser g ca Ala act Th r atc Ile aag Lys cat His cgt Arg gg t Gly gc c Ala gga Gly 150 att Ilie gg t Gly att Ile ttc Phe cgt Arg 230 atc Ile gtt Val1 t ct S er c ca Pro gag Giu gac Asp a tg Met atc Ile 120 cct Pro cc t Pro gg t Giy tac Tyr ctg Le u 200 cg t Arg gta Val1 gc c Ala gtt Val1 tgg Trp gg t Giy gag Giu Gly ga c Asp 105 atc Ilie ggt Gly cat His cg t Arg c tg Leu 185 ctt Leu a ag Lys tgg Trp atc Ile ttg Leu gtt Val1 cct Pro gtg Val gca Ala cct Pro a cg Th r cat His gag Giu cac His 170 aat As n cca Pro tac Tyr gag Giu tac Tyr tgc Cys atc Ile ga a Giu ggt Gly cag Gin t cg Ser atc Ilie acc Th r tcc Ser 155 acc Th r tct Se r gga Gly att Ilie acc Th r 235 ga t Asp agc Ser ccc Pro aac Asn cac His a cc Th r gag Glu cag Gin tat Tyr 145 gtt Val1 acc Th r gag Glu ca g Gin cg t Arg 225 tcc Se r ctg Leu t cg Ser cgg Arg gtC ValI cg t Arg aat As n gag G iu att Ile 130 ttc Phe tct Se r gac Asp cgc Arg gac Asp 210 c tg Leu atg Met cg t Arg ctg Leu agc Ser t tg Le u cac His g ca Ala att Ile 115 gag Giu atc Ile ggc Gly gg c Gly ggc Gly 195 g tg Val1 aac Asn a ag Lys ggt Gly gaa Giu att Ile cat His tat Tyr cct Pro 100 cat His g tg9 Val1 tgg Trp ga c Asp gat Asp 180 aag Lys tcc Ser ca g Gin ga t Asp gca Ala 211 259 307 355 403 451 499 547 595 643 691 739 787 835 883 WO 01/00805 WO 0100805PCTIBOOIOO926 gag cag tcc Giu Gin Ser tcc gcc tca Ser Ala Ser 280 250 255 260 act cat gtg gct att cgt tac ctg cag gct cag gaa gct Thr His Val Ala Ile Arg Tyr Leu Gin Ala Gin Giu Ala 265 270 275 aac taaacacttt taactaaaca aca As n <210> 130 <211> 281 <212> PRT <213> Corynebacteriun giutarnicum <400> 130 Val Thr Lys Leu Giu Arg Met Glu His 1 Pro Se r Pro As n His Th r Giu Gin Ty r 145 Val Thr Giu Gin Arg 225 5 Ser Gly Val1 Ser Asp Val1 Asp Val1 Ser Thr 165 Le u Asp Ser Ile Ala Thr Ile Lys 70 His Arg Gly Ala Giy 150 Ile Gly Ile Phe Arg 230 Pro 10 Leu Val1 Pro Val1 Ala 90 Pro Thr His Giu His 170 As n Pro Tyr Glu Tyr Pro Arg Asp Leu Arg Tyr Asp A rg 140 Th r Th r Leu His Glu 220 Arg Se r As n Ala Giu Ile Ph e Cys Gly 125 Asp Leu Met Ala Gly 205 Arg Gly Gin Pro Pro Gly Leu Arg Ala 110 Val Ser Glu Ile Ile 190 Pro Arg Arg Leu Giy Giu His Leu Gin Gly Th r Val1 Gly Ser 175 Leu Asp Giu Asp Arg Tyr Asp Leu Th r Le u Ala Pro Se r Ile 160 Giu Gi u Giy Leu Vali 240 WO 01/00805 PCTIiBOO/00926 Ser Met Lys Asp Leu Ile Asp Ala Ile Tyr Asp Asp Val Asp Pro Val 245. 250 255 Leu Arg Gly Ala Ala Glu Gln Ser Thr His Val Ala Ile Arg Tyr Leu 260 265 270 Gin Ala Gin Giu Ala Ser Ala Ser Asn 275 280 <210> 131 <211> 1530 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1507) <223> RXN02966 <400> 131 aaatataccc cccagggtat cttqacagat taaagctcga tgttttaggc tctacatata cccccacggq tatcccctca actttgatct aaggtgtcac atg ctt ttt gaa cgc Met Leu Phe Giu atc Ile cgc Arg tat Tyr gaa Giu gc t Ala t gg Trp aag Lys cca Pro ccc gg C Gly gcg Ala g ca Ala cat His g cc Ala ttt Phe atc Ile t ca Ser ctc ctc Leu att Ilie gca Ala gcc Ala gag Glu 75 aca Thr acc Th r ttt Phe agc att Ile gat Asp a gc Ser cgc Arg ggc Gly aat Asn ccc Pro qt c Val1 130 ggt ggc Giy atc Ile qcc Ala gaa Giu gqa Gly tcc Se r gga Gly 115 tca Ser gac tgc Cys ca g Gin gta Val ctt Le u gc t Ala acc Th r 100 cac His aag Lys gtg Arg caa Gin acc Thr acc Th r gca Ala ga t Asp atc Ile act Thr ga t Asp gga 115 163 211 259 307 355 403 451 499 547 wo 01/00805 WO 0100805PCT11BOO/00926 Ph e ga t Asp g ca Ala gac Asp aag Lys 200 aat Asn gtg Val1 cg c Arg t cc Se r tct Ser 280 gga Gly aat As n att Ile t tg Leu ga t Asp 360 gc t Ala Met tta Leu ca g Gin cac His 185 g ca Ala gcg Ala gaa Giu atg Met ccg Pro 265 g aa Glu acc Th r ttt Phe gtt Val1 ctg Leu 345 gga Gly gag Giu Leu ctt Leu ca a Gin 170 att Ile ttg Le u tgg Trp gag Glu aaa Lys 250 ctt Leu gca Al a qtt Va 1 gcg Ala ttg Le u 330 cgg Arg ttg Le u acc Thr Asp Phe gca gct Ala Ala cat agc His Ser tat cca Tyr Pro 190 atc cct Ile Pro 205 cca tat Pro Tyr gac ggc Asp Gly aac aag Asn Lys cta gaa Leu Glu 270 gtg gat Val Asp 285 qca ttg Ala Leu gtt att Val Ile gac gcc Asp Ala atc gac Ile Asp 350 ttt gtg Phe Val 365 gac gca Asp Ala Val1 ggc Gly cta Leu 175 gg t Gly agc Se r ctg Leu cag Gin ca g Gin 255 gcc Ala acc Thr aat Asn gat Asp aat As n 335 act Thr c ca Pro ctg Leu Phe ggc Gly 160 aaa L ys cat His acc Th r cgc Arg cca Pro 240 gga Giy gag Giu cgc Arg at t Ile cct Pro 320 acc Th r gtg Vai ga a Glu att Ile Val1 145 gtg Val1 gag Glu ggt Giy a cc Thr agt Ser 225 gat Asp cct Pro gaa Glu gag Giu ccg Pro 305 caa Gin gct Ala cgt Arg ctc Leu ga t Asp Gly gac Asp ca g Gin gc t Ala gt g Val1 210 gat Asp gcc Ala gca Ala gtc ValI caa Gin 290 cgc Arg aag Lys qcg Ala tat Tyr atc Ile 370 atc Ile Val1 cg c Arg ctt Leu 180 agc Ser tat Tyr gaa G iu gct Ala ctt Leu 260 gaa Giu caa Gin gcc Ala gct Ala ttc Ph e 340 acc Thr ccc Pro g ca Ala Gly ttc Ph e 165 gca Ala cc t Pro ga a Giu gcc Ala tac Tyr 245 agt Ser aag Lys gtc Val1 aag Lys ca g Gin 325 cgc Arg aac As n gct Ala aag L ys 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 WO 01/00805 375 380 tcc gaa ttt gcc gct ggc agc att ccc Ser Giu Phe Ala Ala Gly Ser Ile Pro 390 395 ggt tcg gcc atg tgg cgc ctc aat gag Gly Ser Ala Met Trp Arg Leu Asn Gilu 410 gta acc ttc tgc caa tca gga gcg cga Val Thr Phe Cys Gln Ser Gly Ala Arg 425 430 ttg cga cgc gcc qga ttc acc gtt atc Leu Arg Arg Ala Gly Phe Thr Val Ile 440 445 gcg tgg gaa aaa tca gct gcc aat cct Ala Trp Glu Lys Ser Ala Ala Asn Pro 455 460 tagttttaga tccggcgctg tat <210> 132 <211> 469 <212> PRT <213> Corynebacterium glutamicum <400> 132 PCT/IBOO/00926 9gc Gly ctg Leu 415 aat As n gag G16~ aaa Lys gcg Ala 400 cc t Pro acc Th r ctc Leu aac Asn 385 ca g Gin gcg Ala gtg Val1 gag Glu ttg Leu 465 cag Gin gg t Gly gta Val ggc Gly 450 cag Gin ctt Le u ggc Giy 9C Ala 435 agc S er act Th r 1315 1363 1411 1459 1507 1530 Met Leu Phe Giu 1 Phe Arg Ile Thr Giu His Thr Ala Val 145 Ile Asp Se r Arg Gly Asn Pro Val1 130 Gly Cys Gin Val1 Leu Ala Thr 100 His Lys ValI Arg Gin Th r Thr Ala Asp Ile Th r Asp Gly Ile Tyr Glu Giu Arg Glu Gly Lys 25 Tyr Leu Asp Leu Giu Thr His Ile Ala Ala Thr Gly Trp Gin Tyr Gly Lys Leu Gly Asn 105 Pro Giu His Leu 120 Pro Gly Phe Met 135 Arg Pro Asp Leu 150 Gin Val1 As n Tyr Phe Th r Th r Ile 125 Asp Ala Ala Asp Asn Leu Leu Th r Ala 110 Th r Phe Ala WO 01/00805 Val Asp Thr Arg PCTIBOO/00926 Lys Phe Ala Gly Ala Gin Gin Leu Phe His Ser Leu Giu Gly Thr Ser 225 Asp Pro Giu Giu Pro 305 Gin Ala Arg Le u Asp 385 Gin Ala Val1 Giu Le u 465 165 Leu Ala 180 Ser Pro Tyr Glu Giu Ala Ala Tyr 245 Leu Ser 260 Giu Lys Gin Val Ala Lys Ala Gin 325 Phe Arg 340 Thr Asn Pro Ala Ala Lys Ser Gly 405 Thr Leu 420 Asn Ala Tyr Ala Ala Val 170 175 Pro Gly Ala 215 Phe Ala Leu Gly Le u 295 Ser Leu Ala Ile Leu 375 Glu S er Th r Arg T rp 455 Asp Ly s 200 Asn Val1 Arg Ser Ser 280 Gly As n Ile Leu Asp 360 Ala Ph e Ala Ph e Arg 440 Glu His 185 Ala Ala Giu Met Pro 265 Giu Th r Phe Val Leu 345 Gly Giu Ala Met Cys 425 Ala Lys Ile Le u Trp Giu Lys 250 Le u Al a Val Ala Leu 330 Arg Leu Th r Ala Trp 410 Gin Gly Ser Pro 190 Pro Tyr Gly Lys Giu 270 Asp Leu Ile Ala Asp 350 Val1 Al a Pro Giu Arg 430 Ile Pro Gly Ser Leu Gin Gin 255 Ala Th r Asn Asp Asn 335 Th r Pro Leu Gly Leu 415 As n Giu Lys His Th r Arg Pro 240 Gly Giu Arg Ile Pro 320 Th r Val Giu Ile Ala 400 Pro Thr Leu Asn WO 01/00805 <210> 133 <211> 1530 <212> DNA <213> Corynebacterium giutanicun <2 <221> CDS <222> (101)..(1507) <223> FRXA01833 <400> 133 aaatataccc cccagggtat cttgacagat taaagctcga PCT/IBOO/00926 tgttttaggc tctacatata cccccacggg tatcccctca actttgatct aaggtgtcac atg ctt ttt gaa cgc atc Ile cgc Arg tat Tyr gaa Glu gct Ala tg Trp aag Lys cca Pro ccc Pro cg t Arg 150 gcc Ala tac Tyr gaa Glu ctg Leu a cc Thr gcc Ala caa Gin ctg Leu gag Giu gg t Gly 135 c ca Pro gga Gly gaa Giu Gly qa c Asp cat His acc Th r tat Tyr ga a Giu ca c His 120 ttt Ph e ga t Asp gca Ala gaa Giu aaa Lys ctt Le u att Ile Giy Gly aat As n 105 ctg Le u atg Met tta Le u ca g Gin 9gc Gi y g cg Ala gca Ala cat His gcc Ala ttt Phe atc Ile tca Se r ctc Leu ctt Leu caa Gin 170 ctc Le u att Ile gca Ala gcc Ala gag Giu 75 aca Th r acc Th r ttt Ph e ag c Ser ga t Asp 155 ctc Leu agc Ser 15 gc t Al a a tq Met tcc Ser tct Se r ttg Leu 95 a ag Lys ga t Asp gtc Val ggc Gly cta Leu 175 tat Tyr cgc Arg gtc Val gg t Gi y Gly 80 atg Met ca c His gg t Gly ttc Phe gg c Gly 160 aaa Lys Met 1 ttc Phe cga Arg att Ile act Th r gaa Giu cac His act Th r gcg Ala gta Val1 145 g tg Val1 gag Glu Leu att Ile ga t Asp agc Ser cgc Arg ggc Gly aat Asn ccc Pro gtc Val1 130 ggt Gly ga c Asp ca g Gin Phe ggc Gly atc- Ile q cc Ala gaa Giu gga Gly t CC Ser gga Gly 115 t ca Ser g ac Asp acc Th r ttc Phe Glu tg c Cys cag Gin g ta ValI ctt Le u gct Ala acc Thr 100 cac His aag Lys g tg Val cgc Arg ct t Leu 180 Arg caa Gin acc Thr acc Thr gca Ala ga t Asp atc Ile act Th r gat Asp gga Gly ttc Phe 165 gca Ala 115 163 211 259 307 355 403 451 499 547 595 64 3 WO 01/00805 ctc ccc gac cac att cag gtt tat cca PCT/IBO0100926 Leu tg t Cys aag Lys ggc Gly 230 ttt Phe aca Thr ctt Leu cat His gcg Ala 310 gac Asp gac Asp agt Ser gag Glu tcc Ser 390 gq t Gly gta Asp a ag Lys 200 aat As n gtg Val cgc Arg t cc Ser tct Ser 280 gga Gly aat Asn att Ile ttg Leu gat Asp 360 gct Ala ttt Ph e gcc Al a ttC His 185 gca Ala gcg Al a gaa G lu a tg Met ccg Pro 265 gaa Glu acc Thr ttt Phe gtt Val Ct g Leu 345 gga Gly gag Glu gcc Ala a tg Met tgC Ile Gin Val Tyr Pro 190 cat ggt gct ggc agc cct His Gly Ala Gly Ser Pro 195 t tg Leu t gg T rp gag Glu aaa L ys 250 ctt Le u gca Ala gtt Val gcg Al a ttg Le u 330 cgg Arg ttg Leu acc Th r gc t Ala tgg Trp 410 caa ggc Gly tgg T rp ctt Le u 235 aag Lys gtq Val1 g ta Val1 gtt Val g cg Ala 315 gct Ala gtt Val1 CC t Pro aac Asn ggc Gly 395 cgc Arg t ca acc Thr cg C Arg cca Pro 240 gga Giy gag Glu cgc Arg att Ile CC t Pro 320 acc Th r gtg Val1 ga a Glu att Ile gcg Ala 400 cct Pro acc acc Th r agt Ser 225 ga t Asp ct Pro gaa Giu gag Glu C cg Pro 305 caa Gln gCt Ala cg t Arg ctc Leu ga t Asp 385 cag Gln gcg Ala gtg gtg Val1 210 ga t Asp gcc Ala gca Ala gtc Val caa Gln 290 cg C Arg aag Lys gcg Ala tat Tyr atc Ile 370 atc Ile ca g Gln gg t Gly gta gga Gly ga t Asp cac His gta Val gtC Val1 275 aa c As n ggc Gly ga t Asp ga t Asp ttc Ph e 355 tcc Ser cg t Arg ctt Le u ggC Gly gcc tat Tyr gaa Glu gc t Ala ctt Leu 260 gaa Glu caa Gin g cc Ala gct Ala t tc Phe 340 acc Thr ccc Pro gca Ala tct Ser act Thr 420 aat gaa Glu gcC Ala tac Ty r 245 ag t Ser aag Lys gtc Va 1 aag Lys cag Gln 325 cgc Arg aac As n gct Ala aag Lys gga Gly 405 ttg Le u gc t 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 WO 01/00805 Val Thr Phe Cys Gin Ser Gly Ala Arg 425 430 ttg cga cgc gcc gga ttc acc gtt atc Leu Arg Arg Ala Gly Phe Thr Val Ile 440 445 gcg tgg gaa aaa tca gct gcc aat cct Ala Trp Glu Lys Ser Ala Ala Asn Pro 455 460 tagttttaga tccggcgctg tat <210> 134 <211> 469 <212> PRT <213> Corynebacterium giutamicum <400> 134 Met Leu Phe Giu Arg Ile Tyr Glu Glu PCTLBOO/00926 Asn Thr Val Val Ala Asn Ala 435 gag ctc gag ggc agc tac gcc 1459 Giu Leu Giu Gly Ser Tyr Ala 450 aaa aac ttg cag act gcc gtc 1507 Lys Asn Leu Gin Thr Ala Vai 465 1530 1 Phe Arg Ile Th r Glu His Th r Ala Val 145 Val1 Giu Gly Th r Ile Asp Ser Arg Gly As n Pro Val 130 Gly Asp Gin Ala Val1 210 Gly Ile Ala Giu Gly Ser Gly 115 Ser Asp Th r Phe Gly 195 Gly Cys Gin Val1 Leu Ala Th r 100 His Lys Val1 Arg Leu 180 Ser Tyr 5 Gin Thr Thr Ala Asp Ile Th r Asp Gly Phe 165 Ala Pro Giu Arg Tyr Glu Al a 70 Trp Lys Pro Pro Arg 150 Ala Leu Cys Lys Giu Le u Th r 55 Ala Gin Leu Glu Gly 135 Pro Gly* Pro Giy Ala 215 Gly Asp 40 His Th r Tyr Glu His 120 Ph e Asp Ala Asp Lys 200 As n Ly s 25 Leu Ile Gly Gly As n 105 Le u Met Leu Gin His 185 Al a Ala Gly Ala Ala His Ala Phe .le Ser Leu Leu Gin 170 Ile Leu Trp Leu Ile Ala Ala Glu 75 Th r Thr Phe Se r Asp 155 Le u Gin Gly Trp Ala Val1 Lys Asp Ile Gly Ile Leu Giy 140 Giu Phe Val Ala Ala 220 Gin Vai Asn Tyr Ph e Thr Thr Ile 125 Asp Ala His Tyr Ile 205 Pro Ala Asp Asn Leu Leu Thr Ala 110 Thr Phe Ala Se r Pro 190 Pro Ty r Tyr Arg Va 1 Gly Gly Met His Gly Phe Gly 160 Lys His Thr Arg WO 01/00805 PCT/IB00/00926 Ser Asp Asp Glu Ala Gly Phe Val Glu Glu Leu Leu Asp Gly Gin Pro 225 230 235 240 Asp Ala His Ala Tyr Phe Ala Arg Met Lys Lys Gin Asn Lys Gin Gly 245 250 255 Pro Ala Val Leu Ser Thr Leu Ser Pro Leu Val Lys Leu Glu Ala Glu 260 265 270 Glu Val Val Glu Lys Leu Gly Ser Glu Ala Val Phe Val Asp Thr Arg 275 280 285 Glu Gin Asn Gin Val His Leu Gly Thr Val Val Gly Ala Leu Asn Ile 290 295 300 Pro Arg Gly Ala Lys Ala Ser Asn Phe Ala Ala Trp Val Ile Asp Pro 305 310 315 320 Gin Lys Asp Ala Gin Asp Leu Ile Val Leu Ala Pro Asp Ala Asn Thr 325 330 335 Ala Ala Asp Phe Arg Asp Ala Leu Leu Arg Val Gly Ile Asp Thr Val 340 345 350 Arg Tyr Phe Thr Asn Ser Ile Asp Gly Leu Pro Thr Phe Val Pro Glu 355 360 365 Leu Ile Ser Pro Ala Glu Leu Ala Glu Thr Asn Tyr Asp Ala Leu Ile 370 375 380 Asp Ile Arg Ala Lys Ser Glu Phe Ala Ala Gly Ser Ile Pro Gly Ala 385 390 395 400 Gin Gin Leu Ser Gly Gly Ser Ala Met Trp Arg Leu Asn Glu Leu Pro 405 410 415 Ala Gly Gly Thr Leu Val Thr Phe Cys Gin Ser Gly Ala Arg Asn Thr 420 425 430 Val Val Ala Asn Ala Leu Arg Arg Ala Gly Phe Thr Val Ile Glu Leu 435 440 445 Glu Gly Ser Tyr Ala Ala Trp Glu Lys Ser Ala Ala Asn Pro Lys Asn 450 -455 460 Leu Gln Thr Ala Val 465 <210> 135 <211> 675 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(652) <223> RXA01853 <400> 135 WO 01/00805 PCTEBOO/00926 gaatgtccaa cgtcaaaaaa attcttttct atcctaaccg tatcttcaca ccttgaggqa atgatggggq agcgccgctt acgcactaca ctgtttattc atg gag Met Glu ttc Phe aat As n gtg ValI acc Th r cg t Ar g gag Glu ga c Asp gg t Gly cca Pro ttt Ph e 150 gag Glu ct C Leu g cg Ala gag Glu ga a G lu cac His ttc Phe gtc Val1 aac Asn gag Glu ggg Gly 135 agc Ser t gg Trp cca Pro gct ggt ccg Ala Gly Pro gtc gcg atc Val Ala Ile tac atc acc Tyr Ile Thr 4d gga cac att Gly His Ile aat gcg ccg Asn Ala Pro tac aag gga Tyr Lys Gly 90 atg gtg tcc Met Val Ser 105 aca atc act Thr Ile Thr 120 cat tca cct His Ser Pro ggt gat gtg Gly Asp Val tct gat gca Ser Asp Ala 170 ctt tgatgat Leu tat aaa aca aat Tyr Lys Thr Asn att gat cct ggc Ile Asp Pro Gly 30 acg aat aat ttg Thr Asn Asn Leu 45 gat cac acc cgt Asp His Thr Arg 60 gtc tat atc cat Val Tyr Ile His 75 tct gga aca aag Ser Gly Thr Lys cct gat cct gag Pro Asp Pro Glu 110 ttg gct ggc gaa Leu Ala Gly Glu 125 ggt tgt acg ctg Gly Cys Thr Leu 140 ttg ttc aag ggc Leu Phe Lys Gly 155 gat gcc atg aat Asp Ala Met Asn t gc Cys 15 atg Met agc Ser ga t Asp cct Pro acg Th r 95 t ca Ser gaa Glu atc Ile tct Ser gag .Gl1u 175 tat Tyr cat His gtg Val1 gc t Al a gat Asp 80 gcc Al a ctg Le u ttc Phe gtc Val1 att Ile 160 1 g tg Val1 gcc Ala gac Asp gg t Gly ga t Asp atg Met cg t Arg acg Thr ggc Gly 145 ggt Gi y gtg Val ca C His aag Lys gtt Val1 gcg Ala ctt Leu ga t Asp ttg Leu 130 aag Lys cq c Arg atc ctc gga Ile Leu Gly cgc ggg gag Arg Gly Glu gat gat ttg Asp Asp Leu att gtg ctc Ile Val Leu gtg gca aag Val Ala Lys ttt ttc ctt Phe Phe Leu ttc gac gcc Phe Asp Ala 100 ctg gtt gat Leu Val Asp 115 aag cat gca Lys His Ala gaa tac tgc Glu Tyr Cys act gat ttt Thr Asp Phe 165 115 163 211 259 307 355 403 451 499 547 595 643 tca cta cgc acg gca gtg Ser Leu Arg Thr Ala Val gca ttgcagattc ttc <210> 136 <211> 184 <212> PRT <213> Corynebacterium glutamicum <400> 136 WO 01/00805 PCT/IBOO/00926 Met Glu Ile Leu Gly Phe Ala Ala Gly Pro Tyr Lys Thr Asn Cys Tyr 1 ValI Ala Asp Gly Asp Met Arg Thr Gly 145 Gly Le u Gly Asp Val1 Ala Phe Asp 100 Val His Tyr Asp Al a 180 5 Glu Le u Leu Lys Leu Ala Asp Ala C ys Phe 165 Val1 Giu Giu His Phe Val Asn Glu Gly 135 Ser Trp Pro Val Tyr Gly Asn Tyr Met Thr 120 His Gly Ser Leu 10 Ile Thr Ile Pro Gly 90 Ser Th r Pro Val1 Ala 170 Ile Th r Asp Val1 75 Ser Pro Leu Gly Leu 155 Asp Asp As n His Tyr Gly Asp Ala Cys 140 Ph e Ala Gly Leu Arg His Lys Glu 110 Giu Leu Gly As n Met Ser Asp Pro Th r Ser Glu Ile Ser Glu 175 His Val1 Ala Asp Ala Le u Phe Val1 Ile 160 Ser <210> 137 <211> 723 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(700) <223> RXN02424 <400> 137 agccaatgct caactgccct tcaaaatcga tttttgtcgc ttcaaaaaat acaccttcga acatagcatc gaacaaggtg tttccgctag tctgaaccac atg act aac gag ctc Met Thr Asn Glu Leu 1 act ctt cac cat att tcc gtg tcc caa atg gac aac aat tgc tac ctt Thr Leu His His Ile Ser Val Ser Gin Met Asp Asn Asn Cys Tyr Leu 15 ttg gcc gca aat ggc aac ggt tta ctc att gat gct gca gat gac gca Leu Ala Ala Asn Gly Asn Gly Leu Leu Ile Asp Ala Ala Asp Asp Ala 30 wo 01/00805 WO 0100805PCT/IBOO/00926 gct Ala gtg Val gtt Val cca Pro att Ile acc Th r ctt Leu agc Ser 150 ttt Ph e gaa Giu cg t Arg gca Ala ttg Leu ctc Leu gc t Ala ga a Glu cca Pro ttc Phe 135 gaa Glu gac Asp acc Th r cg c Arg Cta ctt Leu Leu acc acc Thr Thr cag aag Gin Lys ttg ccc Leu Pro ttt gag Phe Glu 105 ggc ggt Gly Gly 120 gtg ggt Val Gly ggc gac Gly Asp acc tac Thr Tyr acc ctt Thr Leu 185 tgg taa T rp 200 aaa tta gcc gaa gat gcc ggt gtg Lys Leu Ala Glu Asp Ala Gly Val 45 acc atc acc aaa Thr Ile Thr Lys cac His acc Thr t cc Se r ggt Gly gca Ala gac Asp ttc Phe ga c Asp 170 gga Gly cg c Arg gga Gly 75 gct Al a cat His gta Val agc Se r gtc Val 155 ga t Asp gcc Ala cac His gca Ala gt t Val1 gta ValI ctc Leu ctc Leu 140 cga Arg ga c Asp gag Glu g ca Ala act Thr ga t Asp ttc Phe acc Thr 125 ttc Phe c tg Leu agc Ser cg t gac Asp cac His gtg Val cct Pro 110 gc t Ala ccc Pro t tc Phe atc Ile cca ccg Pro gag Glu ga t Asp 100 gg c Gi y act Th r acc Thr cgc Arg 9gc Gly 180 t gg T rp gag Glu g tg Val1 tca Ser ca c His cac His agc Ser atc Ile 165 aag Ly s gag Gl u 259 307 355 403 451 499 547 595 643 691 Arg Pro Gin qcgcttt tctcaaccag gca <210> 138 <211> 200 <212> PRT <213> Corynebacterium glutamicum <400> 138 Met Thr Asn Giu Leu Thr Leu His His Ile Ser Val Ser Gln Met Asp 1 5 10 Asn Asn Cys Tyr Leu Leu Ala Ala Asn Gly Asn Gly Leu Leu Ile Asp 25 Ala Ala Asp Asp Ala Ala Ala Leu Leu Lys Leu Ala Glu Asp Ala Gly 40 WO 01/00805 PCT/IBOO/00926 Val Thr Ile Thr Lys Val LeU Thr Thr His Arg His Ala Asp His Val* Arg Pro His Ile Asp Leu 145 Val Pro Glu Pro Giu Asp 100 Gly Thr Thr Arg Gi y 180 Trp Giu Val Ser His His Ser Ile 165 Lys Glu ValI 70 Pro Ile Th r Le u Ser 150 Ph e Glu Arg Leu Ala Giu Pro Phe 135 Giu Asp Th r Arg Gly 75 Ala His Val1 Ser Val1 155 Asp Ala Tyr Giu Ile Glu Gly As n Val 175 Gin Ala Le u Ser Ile Gly Asp 160 Trp Leu <210> 139 <211> 404 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> <223> FRXA02424 <400> 139 acc gga gca act cac tac qcg cct I Thr Gly Ala Thr His Tyr Ala Pro I 1 5 tcc gct gtt gat gtg gaa ctg catc Ser Ala Val Asp Vai Giu Leu His ggt cat gta ttc cct atc agc att Gly His Vai Phe Pro Ilie Ser Ile 1 gca gta ctc acc gct gag atc gac c Ala Val Leu Thr Ala Glu Ile Asp gac agc ctc ttc ccc ggc ggt ttg Asp Ser Leu Phe Pro Gly Gly Leu 70 gct Ala ga a Glu cca Pro ttc Ph e ga a Glu ttg Le u ttt Ph e ggc Gly gtq Val ggc Gly ccc Pro gag Giu ggt Gly gg t Gly qac Asp WO 01/00805 ttc gtc cga ctg ttc aac gat gtc Phe Val Arg Leu Phe Asn Asp Val gac gat gac agc atc gtg tgg cca Asp Asp Asp Ser Ile Val Trp Pro 100 gga gcc gag cgt cca cag ctg gaa Gly Ala Glu Arg Pro Gin Leu Giu 115 120 taagcgcttt tctcaaccag gca <210> 140 <211> 127 <212> PRT <213> Corynebacterium glutamicum <400> 140 Thr Gly Ala Thr His Tyr Aia Pro I 1 Ser Ala Val Asp Vai Glu Leu His 1 Gly His Val Phe Pro Ile Ser Ilie 1 40 Ala Val Leu Thr Ala Giu Ile Asp( Asp Ser Leu Phe Pro Gly Giy Leu( Phe Val Arg Leu Phe Asn Asp Val 1 Asp Asp Asp Ser Ile Val Trp Pro 100 Gly Ala Glu Arg Pro Gin Leu Giu 115 120 aaa gag cgc atc ttt gac acc Lys Glu Arg Ile Phe Asp Thr 90 ggt cac ggc aag gaa acc acc Gly His Giy Lys Giu Thr Thr 105 110 atc tgg tgg gag cgt cgc tgg Ile Trp Trp Glu Arg Arg Trp 125 PCTIBOO/00926 tac 288 Tyr ctt 336 Le u 381 Pro Ile Th r Le u Ser Phe Glu Arg 125 <210> 141 <211> 882 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101) (8 59) <223> RXN00419 <400> 141 gctggttgaa gactcgaaat gagatcgacc caaccggagt ctttgcatct gacatgtccc gccgacttga gctttcttaa gaaagggctt gaactaaaca atg ctt aac gca gtg 115 WO 01/00805 ggc Gly at Ilie t 9 Leu atc Ilie ctc Leu ggc Gly ga a Glu aac Asn gag Glu cag Gin 150 tc Phe gcc Ala gca Ala gati Asp cac His aaa Lys icc Ser gca Ala aaa Lys gac Asp gac Asp g ca Ala tac Tyr cag Gin 135 cga A rg gac Asp aac As n ga t Asp gct Ala 215 cca Pro gcc Ala at Ile g cg Ala g ca Ala acc Th r gtt Val1 cag Gin a cc Th r 120 cag Gin gic ValI ggt Gly gta Val1 ggt Gly 200 gt ValI ctg Leu caa Gin gtc Val1 cg i Arg gc t Al a gaa Giu qac Asp tgg Trp 105 gcc Ala gg C Gly cg c Arg tc Phe i tg Leu 185 ggc Gly tat Tyr ttc Phe ctc Leu ttc Phe icc Ser gct Ala 60 cci Pro aic Ile caa Gin icc Se r acc Th r 140 aac Asn cag Gin cgc Arg cca Pro Val1 220 gic Val1 cii Le u ctc Le u cca Pro 45 tcc Ser gca Al a gig Val1 gca Ala gia Val 125 aic Ile iii P he ctc Leu cca Pro ct 9 Leu 205 gig Val1 ici Ser ct Leu a ag Lys cgc Arg gt Val1 gcc Ala gct Ala cia Leu 110 gg t Gi y gig Val gic Val ggc Gly ggc Gly 190 acc Th r aac Asn iii Phe gg i Gly cag Gin gig ValI gci Ala aic Ile itc Phe gca Ala g ia Val1 g ca Ala iac T yr gaa G iu 175 cag Gin gtc Val1 a ag Lys g cg Ala gga Gly gg i Gly ga c Asp gt Val ga c Asp 80 ggc Gly gig ValI cig Leu tig Leu gg c Gly 160 gcc Ala gia ValI aac Asn aag Lys iic Phe Met Leu 1 acc ict Thr Ser cca icc Pro Ser gca gca Ala Ala git gat Val Asp gca gcc Ala Ala aic cic Ile Leu gaa gca Glu Ala cig ggc Leu Gly 130 icc ici Ser Ser 145 icc gcc Ser Ala cig cgi Leu Arg cgc acc Arg Thr cgc gaa Arg Giu 210 gac aic Asp. Ile 225 caa tic Gln Phe PCTIIBOOIOO926 Val ggt 163 Gly acc 211 Th r gag 259 Giu gcg 307 Ala aac 355 As n aac 403 As n gig 451 Val1 iii 499 Ph e ggc 547 Gly ggi 595 Gly 165 ggi 643 Gly icc 691 Ser gca 739 Ala gic 787 Val cga 835 Arg WO 01/00805 230 235 240 gca atc ttc cgc aag ctg ccg ttc taacggaagt tacggaagtt acq Ala Ile Phe Arg Lys Leu Pro Phe 250 <210> 142 <211> 253 <212> PRT <213> Corynebacterium giutamicum PCT[IBOO/00926 245 882 <400> 142 Met Leu Asn Ala Val Giy Lys Ala Gin Gly Th r Giu Aia Asn Asn Val1 Phe Gly Gly 165 Giy Ser Ala Val Arg 245 Ser Ala Lys Asp Asp Ala Tyr Gin 135 Arg Asp As n Asp Aia 215 Pro Ile Ile Ala Ala Thr ValI Gin Thr 120 Gin Val1 Gly Val1 Gly 200 Val1 Leu Phe ValI Arg Al a Giu Asp Trp 105 Ala Giy Arg Ph e Leu 185 Gi y Tyr Phe Arg Asn Ile 10 Ser Arg Lys Asp Gly Ala Ser His Val Ala 90 Arg Asp Giy Val His Gly Arg Ser 155 Tyr Thr 170 Val Val Giu Ala Asp Ala Gin Tyr 235 Lys Leu 250 Leu Ph e Ser Ala Pro Ile Gin Ser Thr 140 As n Gin Arg Pro Val1 220 Val1 Le u Leu Pro Ser Ala Val Ala Val 125 Ile Phe Leu Pro Le u 205 Val1 Ser Leu Lys Arg Val1 Ala Ala Leu 110 Gly Val1 Val Gly G ly 190 Th r Asn Phe Gly Gin Val1 Ala Ile Phe Ala Val1 Ala Tyr Glu 175 Gin Val1 Lys Ala Pro Phe WO 01/00805 PTIO/02 PCT/IBOO/00926 <210> 143 <211> 485 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (462) <223> FRXA00419 <400> 143 gac aac gaa gca cag Asp Asn Giu Ala Gin 1 5 tgg cgc gac caa Trp Arg Asp Gin cta gca gtg gaa Leu Ala Val Giu gca acc Ala Thr acc gtg aac Thr Val Asn aaa ttt gag Lys Phe Giu acc gcc ggc gtt Thr Ala Giy Val gta ggt gta ctg Val Gly Vai Leu ctg ggc cag Leu Gly Gin tcc tct gtg Ser Ser Val cag cag ggc cac Gin Gin Gly His acc atc gtg gca Thr Ile Val Ala gca ggc Ala Gly cag cga gtc cgc Gin Arg Vai Arg tcc aac ttt gtc Ser Asn Phe Val ggc tcc gcc aag Gly Ser Ala Lys gqt ttc gac ggt Gly Phe Asp Gly tac acc cag ctc Tyr Thr Gin Leu gaa gcc ctg cgt Giu Ala Leu Arg gga Gly tcc ggt gcc aac Ser Gly Ala Asn ttg gtg gtt cgc Leu Val Val Arg ggc cag qta cgc Giy Gin Val Arg acc aag Thr Lys atg tcc gca Met Ser Ala gtg gca gat Val Ala Asp 115 gat ggt ggc gaa gcc cca ctg acc gtc aac cgc gaa gac Asp Gly Gly Glu Ala Pro Leu Thr Val Asn Arg Giu Asp 100 105 110 gct gtt tat gat Ala Val Tyr Asp gtg gtg aac aag Val Val Asn Lys gac atc atc Asp Ile Ile ttt gtc Phe Val 130 cac cca ctg ttc His Pro Leu Phe tac gtc tct ttt Tyr Val Ser Phe ttc caa ttc att Phe Gin Phe Ile cqa gca atc .ttc Arg Ala Ile Phe c gc Arg 150 aag ctg ccg ttc Lys Leu Pro Phe taacggaagt tacggaagtt <210> 144 <211> 154 <212> PRT <213> Corynebacterium glutamicum <400> 144 WO 01/00805 WO 0100805PCTIBOOIOO926 Asp Th r Lys Ala Al a Se r Met Val Phe Pro 145 Asn Giu Ala Gln Trp Arg Asp Gln Ala Leu-Aia Val Giu Ala Thr Val1 Phe Gly Gly Gly Ser Ala Val 130 Arg As n Giu Gin Phe Ala Ala Asp 115 His Ala Tyr Gln Arg Asp Asn Asp 100 Al a Pro Ile Thr Gin Val Gly Val1 Gly Vai Le u Phe Ala Gly Argq Phe Leu Gly Tyr Phe Arq 150 10 Ser Val 25 Thr Ile Asn Phe Gin Leu Arg Pro 90 Pro Leu 105 Val *Val Val Ser Pro Phe Leu Se r Ser Leu Arg Arq 110 Asp Gin Gly Ser Ala Arg Thr Glu Ile Ph e <210> 145 <211> 259 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (259) <223> FRXA00421 <400> 145 gctggttgaa gactcgaaat qagatcgacc caaccggagt gccgacttga gctttcttaa gaaagggctt gaactaaaca ggc aaa gcc caa aac att ctc ctt Gly Lys Ala Gin Asn Ile Leu Leu att tcc att gtc tcc cgc ttc ctc Ile Ser Ile Val Ser Arg Phe Leu ttg gca gcg cgt aaa gat tcc cca Leu Ala Ala Arg Lys Asp Ser Pro 45 ctt ggt gga Leu Gly Gly.

15 aag cag ggt Lys Gin Gly 30 cgc gtg gac Arg Val Asp ctttgcatct gacatgtccc atg ctt aac gca gtg Met Leu Asn Ala Val 1 acc tct gag atc ggt Thr Ser Giu Ile Gly cca tcc cat gtg acc Pro Ser His Val Thr gca gca gtc gca gag Ala Ala Val Ala Glu 115 163 211 259 WO 01/00805 PCTfiBOOIOO926 <210> 146 <211> 53 <212> PRT <213> Corynebacterium glutarnicum <400> 146 Met Leu Asn Ala Val Gly Lys Ala Gin Asn Ile Leu Leu Leu Gly Gly 1 5 10 Thr Ser Giu Ile Gly Ile Ser Ile Val Ser Arg Phe Leu Lys Gin Gly 25 Pro Ser His Val Thr Leu Ala Ala Arg Lys Asp Ser Pro Arg Val Asp 40 Ala Ala Val Ala Glu <210> 147 <211> 861 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(838) <223> RXN02923 <400> 147 gcatctacat tgaaggaatc cacggagcqc gcatcgaaga catcgttgtg gtqaatqaag acggttgtga aaccctcaac aaccagccca aggaactgcg ttg agc att ctt ctc 115 Leu Ser Ile Leu Leu rt a Leu cac His 99C Gly ttg Leu aaa Lys 9gc Gly g ta tct Ser qtt Val1 ga t Asp gcc Ala ct a Leu gac Asp acc gac Asp gtt ValI ctt Le u ca a Gin gct Ala caa Gin gtg 1 qcc Ala cca Pro aca Thr cga Arg gcc Ala gat Asp gtc t tq Leu gct Ala gt t ValI ctt Leu gtt Val1 acc Thr a tg acg Th r g ca Ala cat His gtg Val gc t Ala cat His 100 ctc WO 01/00805 PCT/IB00/00926 Val Glu Ile Ala Thr Val Asp Tyr Thr Ala Gin Val Ser Met Leu Thr 105 110 115 gtg ctt get gat gag ctc cgc gca caa act act cca gca gcg atc gtg 499 Val Leu Ala Asp Glu Leu Arg Ala Gin Thr Thr Pro Ala Ala Ile Val 120 125 130 gcg ttt tcc tcg att gct ggg tgg cgg gcg cgc cgg cct aac tat gtc 547 Ala Phe Ser Ser Ile Ala Gly Trp Arg Ala Arg Arg Pro Asn Tyr Val 135 140 145 tat gga tcc acc aag get ggt ctc gat gca ttt tgc caa ggg ctt gca 595 Tyr Gly Ser Thr Lys Ala Gly Leu Asp Ala Phe Cys Gin Gly Leu Ala 150 155 160 165 gat age ctg cat ggg aca cac gtg cga ttg att att gcg cgt cct ggc 643 Asp Ser Leu His Gly Thr His Val Arg Leu Ile Ile Ala Arg Pro Gly 170 175 180 ttt gtt att ggt tct atg acc acg ggg atg aag cct gct ccg atg tcg 691 Phe Val Ile Gly Ser Met Thr Thr Gly Met Lys Pro Ala Pro Met Ser 185 190 195 gtg tat cca cga gat gtt gcc gca get gtt gtt agt get tat acc tct 739 Val Tyr Pro Arg Asp Val Ala Ala Ala Val Val Ser Ala Tyr Thr Ser 200 205 210 aag aag agg age acg acc ttg tgg att ccg gga cgt ctg cgg gtt ctc 787 Lys Lys Arg Ser Thr Thr Leu Trp Ile Pro Gly Arg Leu Arg Val Leu 215 220 225 gcc tgg att atg cgg atg gtt cct cgt ccg gtg tgg cgg aag atg cca 835 Ala Trp Ile Met Arg Met Val Pro Arg Pro Val Trp Arg Lys Met Pro 230 235 240 245 cgc tagatacccg ttcagccctc aca 861 Arg <210> 148 <211> 246 <212> PRT <213> Corynebacterium glutamicum <400> 148 Leu Ser Ile Leu Leu Leu Gly Gly Thr Ser Asp Ile Ala Gly Glu Ile 1 5 10 Ala Thr Leu Thr Cys His Gly Glu Asp Val Val Leu Ala Ala Arg Arg 25 Pro Glu Ala Ala Gin Gly Leu Ala Glu Asp Leu Arg Gin Arg Gly Ala 40 Thr Ser Val His Val Leu Ser Phe Asp Ala Gin Val Leu Asp Thr His 55 Arg Glu Leu Val Lys Lys Thr Gin Glu Leu Ala Gly Glu Ile Ser Leu 70 75 WO 01/00805 WO 0100805PCTIBOOIOO926 Ala Vai Val Ala Phe Gly Ile Leu Gly Asp Val Pro Arg 145 Cy s Ile Pro Se r Arg 225 Trp Glii Ser Al a 130 Pro Gln Ala Ala Ala 210 Leu Arg Ile Ala 120 Ser S er Leu Ile Pro 200 Arg Ile Ala 105 Asp Ser Thr His Gly 185 Arg Ser Met Asp Th r Giu Ile Lys Gly 170 Se r Asp Thr Arg Gin Giu Arg Ala Giu Thr Val1 Leu Ala Ala 155 Thr Met Val Thr Met 235 Asp Arg Gly 140 Gly His Th r Ala Leu 220 Val Tyr Ala 125 T rp Leu Val1 Th r Al a 205 Trp Pro Thr 110 Gln Arg Asp Arg Gl y 190 Ala Ile Arg <210> 149 <211> 1203 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1180) <223> RXN02922 <400> 149 cccaccgcgg cgcaaqctac cgcctaggcg ctcggaactc gttccatatc cgcccaacta gtttcccagg gagcccactc aca atc atc gac aat ctt gct cca Thr Ile Ile Asp Asn Leu Ala Pro gct gcg cag cgc gaa cag gat cga Ala Ala Gin Arg Giu Gin Asp Arg cag ctt agt gcc ggt ggg ttc act Gin Leu Ser Ala Gly Gly Phe Thr gtc ctc gcg Val Leu Ala 15 gaa ttc agc Giu Phe Ser 30 aaa ctc cgc Lys Leu Arg caccgccact attgatctca atg atc tca ccg caa Met Ile Ser Pro Gin 1 gag atc gca gca acg Glu Ile Ala Ala Thr cgt gat ttg gca aag Arg Asp Leu Ala Lys att cca gtt gaa ttt Ile Pro Val Giu Phe WO 01/00805 WO 0100805PCT[LBOO/00926 ggc Giy gcg Ala t tg Leu aag Lys ttg Leu a ag Lys qgc Giy 150 gat Asp t tg Leu acc Thr cg t Arg cat His 230 gtt Val1 ga c Asp aaa Lys ttt Phe gat Asp agc Ser aaa Lys gga Giy tct Ser gcc Ala ttc Phe 170 tgg Trp ga a Giu gca Ala ttg Leu caa Gin 250 ttt Ph e tat Tyr agc Ser tca Ser 75 tta Leu atc Ile aac Asn tac T yr ga c Asp 155 gcc Aia gac Asp aaa L ys ccc Pro act Th r 235 aac Asn gat Asp gca Ala g tg Val1 Arg gag Giu att Ile acc Thr 130 ttc Phe aaa Lys t ca Se r ttt Phe ga c Asp 210 gcg Ala acc Thr cac His g tg Vali ctg Leu cc t Pro ca c His Giy 115 aag Lys tat Tyr ga t Asp qgc Gly gcc Ala 195 att Ile ca g Gin cg t Arg gga Giy ggt Gly 275 gtg Vali cac His cgc Arg 100 aat Asn atc Ile tcc S er gag Glu gtt Val1 180 t cc Ser gtc Val1 tca Ser gac Asp agt Ser 260 gag Giu g ca Ala ttc Phe acc Th r gcg Ala cga Arg a cg Thr gaa Glu 165 gtt ValI gga Gly acc Th r cat His att Ile 245 gg t Gly gtg Val1 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 ttt cag ggt ttc gca caa Phe Gin Gly Phe Ala Gin 290 WO 01/00805 WO 0100805PCT/IBOO/00926 gaa ctg gat ctt gtc gtc gat aag gca aaa gcc ggc act gct acg gag 1027 Glu Leu Asp Leu Val Val Asp Lys Ala Lys Ala Gly Thr Ala Thr Glu 295 300 305 gtc gat ctg gcc gct gtc gac ctg agc gcc tac caa gcc cag tta gcg 1075 Val Asp Leu Ala Ala Val Asp Leu Ser Ala Tyr Gin Ala Gln Leu Ala 310 315 320 325 gtg gca cct ttg gtg ctg agc cag gcc acc cag gcc ttt gag gtt ggc 1123 Val Ala Pro Leu Val Leu Ser Gin Ala Thr Gin Ala Phe Glu Val Gly 330 335 340 gca gat gcc tta aac ggc ggc cac gtg gct gcc caa tac acg atc gga 1171 Ala Asp Ala Leu Asn Gly Gly His Val Ala Ala Gin Tyr Thr Ile Gly 345 350 355 tcc ttg tca tgagccaaga aattttgagc cat 1203 Ser Leu Ser 360 <210> 150 <211> 360 <212> PRT <213> Corynebacterium glutamicum <400> 150 Met Ilie Ser Pro Gin Thr Ile Ile Asp Asn Leu Ala Pro Val Leu Ala 1 5 10 Glu Ile Ala Ala Thr Ala Ala Gin Arg Giu Gin Asp Arg Giu Phe Ser 25 Arg Asp Leu Ala Lys Gin Leu Ser Ala Gly Gly Phe Thr Lys Leu Arg 40 Ile Pro Val Giu Phe Giy Gly Leu Giy Phe Ser Leu Pro Giu Ala Phe 55 Giu Vai Leu Val Ala Ala Ala Ala Ala Asp Ser Asn Ile Ala Gin Gly 70 75 Leu Arg Pro His Phe Leu Ala Val Glu Ser Leu Leu Ile Ala Pro Tyr 90 Ser Giu His Arg Thr Lys Trp Leu Arg Lys Ile Ala Giu Lys Gly Vai 100 105 110 Val Ilie Gly Asn Ala Leu Thr Glu Val Gly Asn Lys Pro*Gly Glu Leu 115 120 125 Lys Thr Lys Ile Arg Lys Giu Giy Giu Ser Tyr Vai Leu Ser Asp Thr 130 135 140 Lys Phe Tyr Ser Thr Gly Ser Leu Tyr Ala Asp Trp Ile Gin Vai His 145 150 155 160 Ala Lys Asp Glu Giu Asp Gin Asp Val Phe Ala Phe Val Asp Arg Asp 165 170 175 wo 01/00805 WO 0100805PCT/IBOO/00926 Ala Ser Giy Val Vai Leu Val Asp Le u Le u Le u 225 Ile Ser Val1 Gin Giy 305 Gin Ala Gin Phe Asp 210 Ala Th r His Val1 Gly 290 Th r Ala Phe T yr Ala 195 Ile Gin Arg GI y Gly 275 Ph e Ala Gin Giu Thr 355 Thr Arg His 230 Val1 Asp Lys Giu Val1 310 ValI Aia Ser Ser Asp 215 Leu Thr Leu Ala Leu 295 Asp Ala Asp Leu Ph e 200 Tyr Ser Tyr Pro Lys 280 Asp Leu Pro Ala Ser 360 Tyr 185 Phe Thr Thr Val1 Arg 265 Se r Le u Ala Le u Le u 345 Trp Asp Glu Phe Gly Gin Gin Giu Ala Leu Gin 250 Ph e Tyr Val1 Aia Val1 330 Asn Lys Pro Thr 235 Asn Asp Aia Val1 Val1 315 Leu Gly Val1 Ser 220 Gly Arg Pro Val Asp 300 Asp Se r Gly ValI 205 Ala Ile Th r Gin Glu 285 Lys Le u Gin His <210> 151 <211> 516 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (493) <223> RXN03065 <400> 151 gacggcgcca actctgcatt cttcgcctca gcgtgcgtgg cagtgtttgc actgatcgtg qgcttctttg taaagaggcc agcccgctaa gctaggtcgc atg atc agc att gga 115 Met Ile Ser Ile Gly 1 acc gac ctc gtt cac att tcg gcg ttc gca gag cag ctt gca cag ccg 163 Thr Asp Leu Val His Ile Ser Ala Phe Ala Giu Gin Leu Ala Gin Pro 15 gga agt tcc ttc atg gag gtg ttt tcc gca ggg gag cgt cgt aaa gca 211 Gly Ser Ser Phe Met Glu Val Phe Ser Ala Gly Giu Arg Arg Lys Ala 30 WO 01/00805 aat gaa c9c cag gca agc cgc tac gcc Asn Glu Arg Gin Ala Ser Arg Tyr Ala 45 gcg gca aaa gaa tcc ttt att aag gcc Ala Ala Lys Giu Ser Phe Ile Lys Ala cag cca ccc gtg atc 9CC gaa gaa 9CC Gin Pro Pro Val Ile Ala Giu Glu Ala 75 gtt cgc gca qac gcg tgg ggg cgc gtc Val Arg Ala Asp Ala Trp Gly Arg Val ttg gcc gca gtg gtc cgg gaa tcc atc Leu Ala Ala Val Val Arg Glu Ser Ile 105 110 agc atc agc cac gac ggc gac tac gcg Ser Ile Ser His Asp Gly Asp Tyr Ala 120 125 tgactatcca gtagccacgg aga <210> 152 <211> 131 <212> PRT <213> Corynebacterium giutamicum (400> 152 Met Ilie Ser Ile Gly Thr Asp Leu Val 1 5 Gin Leu Ala Gin Pro Gly Ser Ser Phe Giu Arg Arg Lys Ala Asn Giu Arg Gin Leu Ala Gly Arg Trp Ala Ala Lys Glu GIn Ala Ile Tyr Gly Gin Pro Pro Val 70 Trp Arg Asp Ile Glu Val Arg Ala Asp Glu Leu Ala Pro Glu Leu Ala Ala Val 100 105 Phe Ser Ser Ser Leu Ser Ile Ser His 115 120 Arg Ala Cys 130 gag Giu tgg T rp gtg Val1 9c Ala 999 Gi y gtg Val1 gcg Al a 9CC Ala cgg Arg ttg Le u tcc Ser qcg Al a 130 PCT[IBOO/00926 tgg 259 Trp ggc 307 Gly gaa 355 Glu gaa 403 Giu ttg 451 Leu Ala Ala Glu T rp Val1 Ala Gly Val1 Glu Gly His Ser Val1 Ile Gi u Glu WO 01/00805 <210> 153 <211> 543 <212> DNA <213> Corynebacterium glutaniicum <220> <221> COS <22> (520) <223> RXN03132 <400> 153 taacttattt atacataaaa acacaataaa aaccgcacac gcggcga ccctagactg caaaaaatgg ttaggctaac cttatttcca atg aat Met Asn PCT/IBOO/00926 aac ttcaataaat gtg aat caa Val Asn Gin ca g i In ctt Leu aaa Lys cac His cca Pro ctc Leu ttt Phe gac Asp ttg Le u cg c Arg agc Se r tac Tyr atg Met cac His ccc Pro ccc Pro gga Gi y t cc Ser cgc Arg ccc.

Pro cgg Arg cac His ga a Giu cct Pro 120 gcc Ala atc Ile ctc Leu att Ilie gt t Val atc Ile t cc Ser 105 tac Tyr cgc Arg tct Se r gag Giu gcc Ala ttt Phe ga t Asp ccc Pro gcc Ala acc Th r gac Asp ca g Gin cac His gag Giu 75 ctt Le u ga c Asp acc Th r gcc atq aac Ala Met Asn atg gtt agc met Val Ser 30 ctc att tac Leu Ilie Tyr 45 gca gcc cag Ala Ala Gin 60 ctc cag agc Leu Gin Ser cgc ccc gcc Arg Pro Ala acg ctg ccc Thr Leu Pro 110 act gct gtg Thr Ala Val 125 1 gag Giu acc Thr g cc Ala gtg Val qgc Gly t gg T rp ctc Leu caa acc Th r aat As n agc Ser caa Gin gtc Val gac Asp ag t Ser tcc gca Ala ccg Pro gcc Ala tta Leu aag Lys atc Ile gac Asp 115 a cg acc Thr cgc Arg t gg Trp g cc Ala cac His atg Met 100 ctg Leu cca Pro gtc Val1 cgt Ar g ccc Pro a gg Arg g ca Ala gga Gly cg C Arg cg C Arg 115 163 211 259 307 355 403 451 499 Pro Pro Gin Ser Thr tcc gct cac cga tcc aca cat tgacgcttcc aggcgccacc gcg Ser Ala His Arg Ser Thr His <210> 154 <211> 140 <212> PRT <213> Corynebacterium glutamicum <400> 154 WO 01/00805 Met Asn Val Ak 1 Glu Thr Ala T) Thr Asn Pro Aj Ala Ser Ala Ti Val Gin Leu A.

Gly Val Lys HJ Trp Asp Ile M( ic Leu Ser Asp L( 115 Gin Ser Thr Pi 130 Gly Ser Arg 40 Pro Arg His Giu Pro 120 His <210> 155 <211> 461 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> <223> RXN03157 <400> 155 ggc cac ctc gac atc gtg acc cgc Gly His Leu Asp Ile Val Thr Arg 1 5 acc atc ttg gtc acc gcc aac ccc Thr Ile Leu Val Thr Ala Asn Pro gtc gca gaa cgc atg gat ctc atc Val Ala Glu Arg Met Asp Leu Ile 40 aac gta aaa gta gac act tgg gcc Asn Val Lys Val Asp Thr Trp Ala 55 gaa cat ggc atc ggc gcc cta gtc Glu His Gly Ile Gly Ala Leu Val 70 tac gaa tac gag ctc ccc atggca Tyr Glu Tyr Glu Leu Pro Met Ala Ala Ile Leu Ile Va1 Ile Ser 105 Tyr Ar g cc Ala aac %s n 25 zgc A.rg tca Ser ~aaa Lays cag 31in Arg Ser Giu Ala Phe Asp Pro Ala Ser gcc Ala aaa Lys ga a Giu ctg Leu 9gc Gly atg Met Th r Asp Gin His Giu 75 Leu Asp Thr Th r gcg Ala aac Asn t CC Ser ctc Leu ctg Le u aac Asn Ala Met Leu Al a Leu Arg Thr Th r His 140 ca a Gin t ca Se r acc Thr gtg Val cga Arg a gg Arg Met ValI Ile Al a Gin Pro Leu Al a 125 ttt Ph e gga gca Ala ga t Asp agc Ser cga Arg As n Se r Tyr Gin Se r Ala Pro 110 Val1 agc Ser ctg Leu cac His tac Tyr tC Ser ct C Le u PCTTBOO/00926 Pro Giu Thr Ala Giu Giu Leu Pro WO 01/00805 WO 0100805PCTIBOOIOO926 gta gac acc tic tic ctc ctc acc gac Val Asp Thr Phe Phe Leu Leu Thr Asp 100 105 tcc aca ctg tgc aaa gaa qit gcc cgc Ser Thr Leu Cys Lys Giu Val Ala Arg 115 120 ctc cta cct gag gta gtg gca aaa gcc Leu Leu Pro Giu Val Val Ala.Lys Ala 130 135 cag cac tagticttt tgcatcaagc qic Gin His 145 <210> 156 <211> 146 <212> PRT <213> Corynebacterium glutamicum <400> 156 Gly His Leu Asp Ile Val Thr Arg Ala 1 5 Thr Ile Leu Val Thr Ala Asn Pro Asn Vai Ala Giu Arg Met Asp Leu Ile Arg Asn Val Lys Val Asp Thr Trp Ala Ser Glu His Gly Ile Gly Ala Leu Val Lys 70 Tyr Giu Tyr Giu Leu Pro Met Ala Gin Val Asp Thr Phe Phe Leu Leu Thr Asp 100 105 Ser Thr Leu Cys Lys Giu Val Ala Arg 115 120 Leu Leu Pro Giu Val Vai Ala Lys Ala 130 135 Gin His 145 <210> 157 <211> 1206 <212> DNA <213> Corynebacterium glutamicum 90 gag aaa tac ggc tac gtc Giu Lys Tyr Giy Tyr Val 110 tic ggt ggc gat gic tcc Phe Gly Gly Asp Val Ser 125 gtg aca gaa aaa tac agc Val Thr Giu Lys Tyr Ser 140 agc Se r ggc Gly aac Asn Al a L ys Giu Leu Gly Met Giu Phe Val Val Thr Asp Thr Asp Gly Se r Gi y Asn WO 01/00805 WO 0100805PCTIIBOO/00926 <220> <221> CDS <222> (101)..(1183) <223> RXN00934 <400> 157 ccaacccctg tggtttggtg atttggatcc ggagcgtctc aagcgctcta gggagcagac aaatgttcac aaaccggtgg cattacagga ggacaattag gtg cga att gga atg Vai Arg Ile Gly Met gtc Val1 ctt Le u ctt Leu gg t Gly a gc Ser ggc Gly t cc Se r cac His ctt Le u atg Met 150 atc Ile gaa Glu tcc ga t Asp ttc Phe ga t Asp att Ile ttc Phe cat His tt Phe tcg Ser aa a -Lys 140 gtg Val acc Th r gag Glu atc 1 gtt Val cat His ga t Asp t cg Ser acg Th r aat Asn atc Ile qct Ala atc Ile 145 99c Gly gcc Ala gg t Gly ctg cat His ca g Gin gtg Val1 cgc Arg cg c Arg agt Ser 100 act Th r cca Pro tct Ser gt q Val1 ca a Gin 180 ga t Asp ctg atc le gtg Val1 cgc Arg t tg Le u gaa Glu ttt Phe tac Tyr gq Val gaa G iu ctq Le u 165 atc Ile gag G lu ga t 163 211 259 307 355 403 451 499 547 595 643 691 739 WO 01/00805 Ser Arg Lys Gly 200 cqc ccg ttt acc Arg Pro Phe Thr 215 gcc ggc atc aac Ala Gly Ile Asn 230 atc tta ggt cgc Ile Leu Gly Arg agc ttc ggc aic Ser Phe Gly Ile 265 gtc gcc agc gac Val Ala Ser Asp 280 qca cag cca gcg Ala Gin Pro Ala 295 gcc aaa aaa ctt Ala Lys Lys Leu 310 ctt atc gcc gcg Leu Ile Ala Ala gta tcc acc cag Val Ser Thr Gin 345 gtg agg ctt gga Vai Arg Leu Giy 360 Le u t gc Cys ttt Phe g ca Ala 250 gtcj Val cta Leu qgC Gly caa Gin ggg Gi y 330 gtc Asp acc Th r gtg Val1 235 ga c Asp ct a Le u ga a Glu gtg Val1 gcg Ala 315 cta Le u atg Ile gtc Val1 220 ggc Gly atc Ile gtt ValI gc9 Ala cta Le u 300 ctt Leu aag Lys gca Le u 205 att Ile cgc Arg tat Tyr gaa G iu ttc Phe 285 ttt Phe atc Ile cg C Arg gtc Le u ggc Gly gtc Val1 gtc Val qg Ala 270 t CC Se r aaa Lys gac Asp g ca Ala tat Tyr 350 Arg Ala Leu ggc ggc acc Gly Gly Thr 225 agc qat gag Ser Asp Glu 240 gca ccc aac Ala Pro Asn 255 atg qcc gcg Met Ala Ala ctg gtc acc Leu Val Thr acc ggc tca Thr Gly Ser 305 gac Ccc tcc Asp Pro Ser 320 aac gcc tac Asn Ala Tyr 335 gaa acc att Glu Thr Ile Leu gaa Giu gca Ala Gi y 260 gct Ala ga a Giu gac Asp tcc Ser t cg Ser 340 gac Asp PCT/TBOO/00926 Asp gtc 787 ValI gca 835 Ala 245 gaa 883 Glu gtc 931 ValI gcc 979 Ala cta 1027 Leu acg 1075 Thr 325 aca 1123 Thr aaa 1171 Lys Val Met Ala Val tgacccttgt ttacctcctc atc 1206 <210> 158 <211> 361 <212> PRT <213> Corynebacterium giutamicum <400> 158 Val Arg Ile Gly Met Val Cys Pro Tyr 1 5 Val Gin Ala His Ile Leu Asp Leu Ala 25 His Giu Val Gln Val Leu Gly Pro Cys 40 Asp Phe Val Val Arg Gly Gly Gly Ser 55 Se r 10 Arg Ser Ile Phe Asp Giu Pro Gly Gly Thr Phe Ile Ala Gln Gly Ala Asp Thr Gin Val Pro Pro Ile Pro Tyr Asn Gly WO 01/00805 PTIOIO2 PCT/IBOO/00926 Ser Val Ala Thr Phe Leu Asn Ser Pro Ile Val Ala 115 Ala Phe Leu 130 Ile Ala Val 145 Gly Asp Pro Ala Ala Arg Gly Arq Leu 195 Leu Thr Arq 210 Thr Pro Arg 225 Glu Glu Lys Asn Thr Gly Ala Gly Cys 275 Thr Asp Ser 290 Ser Asp Ala 305 Ser Ser Arg Tyr Asp Trp Ilie Ala Ile 355 <210>' 159 <211> 1320 Arg Leu Ser 70 Arg Glu Gly Ser Phe Ser 100 Thr Tyr His Pro Val Leu Ser Glu Met 150 Val Leu Ile 165 Gin Ile Glu 180 Asp Giu Ser Leu Asp Arq Glu Val Ala 230 Ala Ala Ile 245 Gly Giu Ser 260 Ala Val Val Glu Ala Ala Asp Leu Ala 310 Ser Thr Leu 325 Ser Thr Vai 340 Asp Lys Val Phe Giy Pro Lys Met Phe Lys Aia Vai Arg 75 Asp Ala 105 Se r Met Arg Gi y Asp 165 Gly Thr Asn Arg Ile 265 Asp Al a Leu Ala Gin 345 Gly Val1 90 Leu Ser Leu Trp Val 170 Pro Le u Cys Ph e Aia 250 ValI Leu Gly Gin Gly 330 Val Leu Arg Gly Giu Gin 155 Glu Val1 Asp Th r Val1 235 Asp Leu Giu Val1 Al a 315 Le u Met His Phe Ser Lys 140 Val Th r Giu Ile Val1 220 Gi y Ile Val1 Ala Le u 300 Leu Lys Ala Ile Ala L ys 125 Val Glu Ser Ile Leu 205 Ile Arg Tyr Glu Phe 285 Phe Ile Arg Val1 His Glu 110 Leu Arg Gin Met Val1 190 Leu Gly Val1 Vali Ala 270 Ser Lys Asp Ala T yr 350 Giu Gly Leu Ala ValI Phe 175 Phe Arg Giy Se r Ala 255 Met Le u Th r Asp Asn 335 Glu Pro Pro L ys Gly Gly 160 Lys Le u Ala Gly Asp 240 Pro Al a Vai Gly Pro 320 Ala Th r WO 01/00805 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1297) <223> RXN00792 <400> 159 cacctttggt gctgagccag gccacccagg cctttgaggt PCTIBOOIOO926 tggcgcagat gccttaaacg gcggccacgt ggctgcccaa tacacgatcg gatccttqtc atg agc caa gaa att Met Ser Gin Glu Ile t tg Leu gag Glu gta Val1 g cg Ala ggc Gly gtg Val1 ctg Leu aaa L ys act Th r a cc Th r 150 aac As n gcg Ala cat His gaa Giu cta Le u gc t Ala ga t Asp cta Le u gaa Glu 99c Gly 120 aa c As n acc Thr aac Asn ggc Gly ttt Phe ca g Gin gg t Gly t cc Ser tcc Ser ctc Leu g tc Val1 105 gtt Val1 gga Giy tat Tyr ggc Gly gtg Val gca Ala cag Gin ttt Ph e ctg Le u aat Asn ct g Leu gcc Ala tac Tyr ccg Pro ttt Ph e caa Gin 170 gat Asp ccc Pro cgc Arg act Thr gaa Giu atc Ile 75 ga a Giu a cc Thr ggc Gly att Ile g cg Ala 155 ga a Giu gt t ValI gag Giu cca Pro 30 cga Arg gtt Val1 gcc Ala gag Gi u ctt Leu 110 cag Gin ctc Leu acc Thr gtc ValI gat Asp cgc Arg gtg ValI g tg Val1 gag Glu ctg Leu a gc Ser gtg Val1 acc Thr aac As n t gg Trp ag t Ser 175 tgg Trp att Ile ga a Glu ccc Pro tta Le u cg c Arg 80 gaa Giu Gly ttc Ph e ggc Gly acc Thr 160 t tg Le u agc Ser 1 cga Arg gag Glu gaa Glu ctg Leu gga Gly ttc Phe aac Asn atc Ile act Thr 145 acc Thr c cg Pro ggg Gly agc Ser att Ile ga a Giu atc Ile cat His cg c Arg gcc Al a gat Asp 130 aa g Lys gcg Ala gtc Val ttt Ph e g9 C Gly aaa Lys cta Leu g agc Giu ttt Phe acc Thr gaa Glu 115 gag Giu ttt Phe ctg Leu ga t Asp ggg Gly gt c Vali ctg Leu ggt Gly gcg Ala ttc Phe tgg Trp gag Glu gag Glu acc Thr cgc Arg 165 cac His aag Lys 115 163 211 259 307 355 403 451 499 547 595 643 691 WO 01/00805 WO 0100805PCT/IBOO/00926 ctc Leu egg Arg etg Leu 230 gtc ValI aac As n gg c Gly gce Ala ttc Phe 310 gga Gly tct Ser cg t Arg ggg Gly acc Thr tgg Trp 215 cag Gin gat Asp cct Pro ga c Asp ace Thr 295 aa c As n caa Gin g ca Ala ace Th r gag Glu 375 gc Ala 200 ate Ile ctg Leu gag G iu G ly gea Ala 280 ege Arg gag Glu get Ala gtg Val1 gtg Val1 360 tat Tyr gga aeg ace acg ttt aaa Thr 205 ga t Asp gtg Va 1 gee Aia age Ser ace Thr 285 gee Aia gt t Val tee Ser tee Ser ccg Pro 365 aet Thr Phe geg Aia ctg Le u caa Gin ga t Asp 270 gtt Val1 gee Al a gca Ala aae As n att Ile 350 gt g Vai eet Pro etg gag Leu Glu 210 etg gtg Leu Val 225 agt gee Ser Ala acc aga Thr Arg gca ac Aia Thr gga gcg Giy Aia 290 gta ac Vai Thr 305 tgg ccc Trp Pro gat gcg Asp Ala eac tgg His Trp gee aag Ala Lys 370 ace aac gtg gat eeg 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 Gly Thr Asn Ile Gly Lys 385 tagacgtgtg atttegctgg gea ctg age cgt eeg gtg age eta age age Ala Leu Ser Arg Pro Val Ser Leu Ser Ser 390 395 ttt <210> 160 <211> 399 <212> PRT <213> Corynebacterium glutamieum 1317 1320 WO 01/00805 WO 0100805PCT/EBO0100926 <400> 160 Met Ser Gin Giu Ile Leu Ser His Phe Ala Pro Ala Leu Giu Arg Ile 1 5 10 Arq Ser Gly Ala Val Giu Arg Giu Gin Gin Arg Ala Leu Pro Val Giu 25 Giu Ile Lys Giu Leu Val Giu Leu Gly Phe Thr Giy Leu Arg Val Pro 40 Giu Giu Leu Gly Gly Ala Gly Ala Ser Leu Giu Ser Val Val Giu Leu 55 Leu Ile Glu Ile Ala Gly Ala Asp Ser Asn Ile Ala Gin Ala Leu Arg 70 75 Giy His Phe Ala Phe Val Giu Leu Leu Leu Giu Ala Pro Giu Ser Giu 90 Phe Arg Thr His Trp Leu Arq Giu Val Ala Thr Gly Arg Leu Val Gly 100 105 110 Asn Ala Giu Ser Giu Lys Arg Gly Val Tyr Gly Asp Pro Gin Thr Phe 115 120 125 Ile Asp Giu Val Giu Thr Giu Asn Gly Pro Ile Phe Val Leu Asn Gly 130 135 140 Thr Lys Phe Tyr Thr Thr Gly Thr Tyr Phe Ala Asp Tyr Thr Trp Thr 145 150 155 160 Thr Ala Leu Leu Arg Asn Leu Asn Gly Gin Giu Thr Leu Val Ser Leu 165 170 175 Pro Val Asp Leu His Ala Pro Gly Val Asp Val Ala Asp Asp Trp Ser 180 185 190 Giy Phe Gly Gin Lys Leu Thr Ala Ser Gly Thr Thr Thr Phe Lys Asp 195 200 205 Leu Glu Val Asp Pro Arg Trp Ile Ile Pro Arg Thr Asp Ala Pro Thr 210 215 220 Leu Val Trp Thr Tyr Leu Gin Leu Ser Leu Leu Thr Val Leu Vai Gly 225 230 235 240 Ser Ala Ala Ala Ala Val Asp Giu Val Val Aia Arg Ala Gin Ser Ser 245 250 255 Thr Arg Asn Ala Trp Asn Pro Gly Val Giu Arg Arg Ser Asp Pro Ala 260 265 270 Ala Thr Ile Ala Ile Gly Asp Ala Arg Ser Arg Val Thr Val Ile Arg 275 280 285 Giy Ala Leu Leu Asp Ala Thr Arg His Val Ser Asn Ala Ala Thr Ile 290 295 300 Vai Thr Pro Giu Ala Phe-Asn Giu Ala Asp Ala Ile Val Ala Ala Leu 305 310 315 320 WO 01/00805 WO 0100805PCT/IBOO/00926 Trp Pro Ile Val Ser Gly Gin Ala Leu Val Val Thr Ser Asn Val Phe 325 330 .335 Asp Ala Val Gly Ala Ser Ala Val Leu Gly Giu His Ser Ile Asp Arg 340 345 350 His Trp Arg Asn Val Arg Thr Val Ser Ser Asn Asn Pro Val Phe Leu 355 360 365 Ala Lys Asn Ala Val Gly Glu Tyr Ala Leu Asn Gly Thr Pro Val Gly 370 375 380 Thr Asn Ile Gly Lys Ala Leu Ser Arg Pro Val Ser Leu Ser Ser 385 390 395 <210> 161 <211> 969 <212> DNA <213> Corynebacteriun glutamicun <220> <221> CDS <222> (946) <223> RXN00931 <400> 161 ccgtaaccta atcgttgaaa catcacctta ttgctgggct ttgcacgcta ctctttgtga gtaacctcac cgaagtgcat aaattaattg ggagtgatca ttg Leu tat T yr g ca Ala 9gc Gly gig Val1 Gly cg t Arg gaa Giu ita Leu g ca Ala tac Tyr ga t Asp atc Ile 999 Gly ga a Giu gcc Ala a cg Th r ttt Ph e cga Arg caa Gin ga t Asp aaa act att Lys Thr Ile tac cgt ggt Tyr Arg Gly cag gtc gct Gin Val Ala gcc ttt act Ala Phe Thr cca aca gca Pro Thr Ala agc tac gtc Ser Tyr Val tic agc atg Phe Ser Met 100 atg gac aag Met Asp Lys 115 WO 01/00805 PCT/IB00/00926 cgt aaa gtt cca gct cct gat gag ate aag gga aca gta gaa cgt atg 499 Arg Lys Val Pro Ala Pro Asp Glu Ile Lys Gly Thr Val Glu Arg Met 120 125 130 ccg ate tea agt agg cga gtg ctt gat gaa tgg gcg gaa tgg gat atc 547 Pro Ile Ser Ser Arg Arg Val Leu Asp Glu Trp Ala Glu Trp Asp Ile 135 140 145 cgc gtt att ccg cag gat caa tta gaa ctc age gat ttc acc get act 595 Arg Val Ile Pro Gin Asp Gin Leu Glu Leu Ser Asp Phe Thr Ala Thr 150 155 160 165 gag caa get gtg tgg att cgg tgc acc get gat ctt ccg gat aat ccc 643 Glu Gin Ala Val Trp Ile Arg Cys Thr Ala Asp Leu Pro Asp Asn Pro 170 175 180 acc ttc cac cag tgc tca ctg act tat ctg tcc gat atg act ttg ctg 691 Thr Phe His Gin Cys Ser Leu Thr Tyr Leu Ser Asp Met Thr Leu Leu 185 190 195 cat agt gcc ctg gtg cca cac cca ggt gag aaa atg cag atg gcc tca 739 His Ser Ala Leu Val Pro His Pro Gly Glu Lys Met Gin Met Ala Ser 200 205 210 ctt gat cac gct gtg tgg ttc ctg cgt ccc ttc cgc gtc gat gaa tgg 787 Leu Asp His Ala Val Trp Phe Leu Arg Pro Phe Arg Val Asp Glu Trp 215 220 225 ttg ctt tat gat cag cgc tct cca tcg gcc tea agt ggg cga gcc ttg 835 Leu Leu Tyr Asp Gin Arg Ser Pro Ser Ala Ser Ser Gly Arg Ala Leu 230 235 240 245 act cac ggg egg ctt ttc aac cag cag gga gat ttg gtc gct att gtc 883 Thr His Gly Arg Leu Phe Asn Gin Gin Gly Asp Leu Val Ala Ile Val 250 255 260 aat caa gag gga atg acc cgc aca etc cac gag ggt gcg caa tea att 931 Asn Gin Glu Gly Met Thr Arg Thr Leu His Glu Gly Ala Gin Ser Ile 265 270 275 ccg atg cgc aaa gac taaaatgcag cgaacttgaa gat 969 Pro Met Arg Lys Asp 280 <210> 162 <211> 282 <212> PRT <213> Corynebacterium glutamicum <400> 162 Val Lys Thr Ile Glu Asp Ile Leu Thr Leu Glu Glu Ile Asp Arg Asp 1 5 10 Ile Tyr Arg Gly Pro Val Ile Glu Ser Tyr Leu Ala Arg Thr Phe Gly 25 Gly Gin Val Ala Ala Gin Ala Leu Val Ala Ala Thr His Thr Val Asp 40 WO 01/00805 WO 0100805PCTIBOO/00926 Lys Ala Phe Thr Val His Ser Leu His Gly Tyr Phe Ile Ala Pro Gly Ala Val Met 100 Lys Arg Asp Ala As n 180 Le u Al a Glu Al a Ile 260 Pro Thr Gin Met Met Ile Thr 165 Pro L eu S er T rp Leu 245 Vali Asp Ile Gly Pro Arg Asp 155 Ile Ser Pro Trp Arg 235 Phe Th r Ar g Gin Asp Asp Vali 140 Gln Arg Le u His Ph e 220 Se r Asn Arg Ser Ile Pro Met Arg Lys Asp 280 <210> 163 <211> 969 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (946) <223> FRXA00931 <400> 163 ccgtaaccta atcgttgaaa catcacctta ttgctgggct ttgcacgcta ctctttgtga gtaacctcac cqaagtgcat aaattaattg ggagtgatca gtg aaa act att gaa 115 Val Lys Thr Ile Glu WO 01/00805 PCTIBOO/00926 1 gat att ttg acc ttg gaa gaa atc gac cgc gat att tac cgt ggt ccc 163 Asp Ile Leu Thr Leu Glu Glu Ile Asp Arg Asp Ile Tyr Arg Gly Pro 15 gtt ate gaa tct tat tta gcc agg act ttc ggt ggc cag gtc get gcc 211 Val Ile Glu Ser Tyr Leu Ala Arg Thr Phe Gly Gly Gin Val Ala Ala 30 caa gct tta gta gca gca acg cat act gtt gat aaa gcc ttt act gtg 259 Gin Ala Leu Val Ala Ala Thr His Thr Val Asp Lys Ala Phe Thr Val 45 cat tct ttg cat ggc tac ttt ata gct cct ggt gat cca aca gca ccc 307 His Ser Leu His Gly Tyr Phe Ile Ala Pro Gly Asp Pro Thr Ala Pro 60 gca att tat tta gtg gat cga gtt cgc gac gga aaa age tac gtc acc 355 Ala Ile Tyr Leu Val Asp Arg Val Arg Asp Gly Lys Ser Tyr Val Thr 75 80 cgc tcg gtg cgt ggc atc caa gac ggc gaa gta atc ttc agc atg cag 403 Arg Ser Val Arg Gly Ile Gin Asp Gly Glu Val Ile Phe Ser Met Gin 95 100 gcc age ttt cat cgt ggg gat gaa ggc att gag cac atg gac aag atg 451 Ala Ser Phe His Arg Gly Asp Glu Gly Ile Glu His Met Asp Lys Met 105 110 115 cgt aaa gtt cca gct cct gat gag atc aag gga aca gta gaa cgt atg 499 Arg Lys Val Pro Ala Pro Asp Glu Ile Lys Gly Thr Val Glu Arg Met 120 125 130 ccg atc tca agt agg cga gtg ctt gat gaa tgg gcg gaa tgg gat atc 547 Pro Ile Ser Ser Arg Arg Val Leu Asp Glu Trp Ala Glu Trp Asp Ile 135 140 145 cgc gtt att ccg cag gat caa tta gaa ctc age gat ttc acc get act 595 Arg Val Ile Pro Gin Asp Gin Leu Glu Leu Ser Asp Phe Thr Ala Thr 150 155 160 165 gag caa get gtg tgg att cgg tgc acc gct gat ctt ccg gat aat ccc 643 Glu Gin Ala Val Trp Ile Arg Cys Thr Ala Asp Leu Pro Asp Asn Pro 170 175 180 acc ttc cac cag tgc tca ctg act tat ctg tcc gat atg act ttg ctg 691 Thr Phe His Gin Cys Ser Leu Thr Tyr Leu Ser Asp Met Thr Leu Leu 185 190 195 cat agt gcc ctg gtg cca cac cca ggt gag aaa atg cag atg gcc tca 739 His Ser Ala Leu Val Pro His Pro Gly Glu Lys Met Gin Met Ala Ser 200 205 210 ctt gat cac get gtg tgg ttc ctg cgt ccc ttc cgc gtc gat gaa tgg 787 Leu Asp His Ala Val Trp Phe Leu Arg Pro Phe Arg Val Asp Glu Trp 215 220 225 ttg ctt tat gat cag cgc tct cca tcg gcc tca agt ggg cga gcc ttg 835 Leu Leu Tyr Asp Gin Arg Ser Pro Ser Ala Ser Ser Gly Arg Ala Leu 230 235 240 245 WO 01/00805 act cac ggg cgg Thr His Gly Arg aat caa gag gga Asn Gin Glu Gly 265 ccg atg cgc aaa Pro Met Arg Lys 280 PCTIBOOIOO926 ctt ttc aac cag cag gga gat ttg gtc gct att gtc 883 Leu Phe Asn Gin Gin Gly Asp Leu Val Ala Ile Val 250 255 260 atg acc cgc aca ctc cac gag ggt gcg caa tca att 931 Met Thr Arg Thr Leu His Giu Gly Ala Gin Ser Ile 270 275 gac taaaatgcag cgaacttgaa gat 969 Asp <210> 164 (211> 282 <212> PRT <213> Corynebacterium glutamicum <400> 164 Vai Lys Thr Ile Glu Asp Ile Leu Thr 1 Ile Gi y Lys Asp Lys Ile His Th r Aia 145 Asp Leu Asp Glu Le u Le u Tyr ValI Phe Vai 120 Se r Ile Ala His Ala 200 Leu 10 Tyr Al a Giy ValI Gly Arg Ala Arg Gin Trp 170 Cys Val1 Ile Arg His Ile Val1 Asp Glu Glu 125 Leu Leu Cys Thr Pro 205 Asp Thr Thr Aia Arg Gly Gly 110 Ile Asp G16 Thr Tyr 190 Giy Arg Phe ValI Pro Asp Glu Ile Lys Giu Leu Ala 175 Leu Giu Asp Gly Asp Gly Gly Val Giu Gly Trp Ser 160 Asp Ser Lys Met Gin Met Ala Ser Leu 210 Asp 215 His Ala Val Trp Phe Leu Arg Pro Phe 220 WO 01/00805 PCT[IEBOO/00926 Arg Val Asp Giu Trp Leu Leu Tyr Asp Gin Arg Ser Pro Ser Ala Ser 225 230 235 240 Ser Gly Arg Ala Leu Thr His Gly Arg Leu Phe Asn Gin Gin Gly Asp 245 250 255 Leu Val Ala Ile Val Asn Gin Giu Gly Met Thr Arg Thr Leu His Giu 260 265 270 Gly Ala Gin Ser Ile Pro Met Arg Lys Asp 275 280 <210> 165 <211> 510 <212> DNA <213> Corynebacterium giutarnicum <220> <221> CDS <222> (101) (4 87) <223> RXN01421 <400> 165 ttgaiqcacg tgcagaaatc gtcggcgqtc cgtggcaccc atctgttaag ggagactcgg ttactgcagg gatcctgcga qatcgagtaa acgcctaaag aig acg tcg aaa agc Met Thr Ser Lys Ser att Ile ci c Leu g tg Val1 gt c Val icc Ser aat Asn cac His act Thr a gc Ser gcg Ala tat Tyr ccc Pro gga Gly gtg Val1 tig Leu aca Thr gqc aag Gly Lys gcg cic Ala Leu ccg ttc Pro Phe aig cag Met Gin ttt itg Phe Leu ctg tat Leu Tyr att gcg Ilie* Ala 105 ggc att Gly Ile ctg Leu ttt Phe ga a Giu gct Ala tca Ser cgc Arg tt Ph e c tg Leu 1 icg ctc act Ser Leu Thr cat gcg itg His Ala Leu itt gcc aca Phe Ala Thr gia acc tic Val Thr Phe agc cag cic Ser Gin Leu 80 aic acc aag Ile Thr Lys gag gcg tcg Glu Ala Ser tig cgc gag Leu Arg Giu cgg Arg ttq Leu aaa Lys aic Ile atg Met ccc Pro 100 tic i gg Trp t cq Ser ttt Phe tig Leu aag L ys aig Met a cg Lys Phe Thr taaagctgtg WO 01/00805 120 125 gctgcggaat gcg <210> 166 <211> 129 <212> PRT <213> Corynebacterium glu~amicum <400> 166 Met Thr Ser Lys Ser Ile Ser Gly Lys 1 Thr Gly Ala Arg Trp, Leu Ala Ala Leu Leu Val Phe Leu Ser Val Tyr Pro Phe 40 Thr Ile His Lys Phe Val Pro Met Gin 55 Phe Phe Phe Ile Leu Ser Gly Phe Leu 70 Leu Lys Gly Met Lys Asn Val Leu Tyr Lys Ile Tyr Pro Met His Leu Ile Ala 100 105 Ser Ala Lys Phe Thr Thr Thr Gly Ile 115 120 Glu PCT/IBOO/00926 510 Pro Val Gin Gly Tyr Cys Pro Trp As n Tyr Ser Ser T rp Lys Met ValI Le u Phe Giu Ala Ser Arg Ph e Le u 125 <210> 167 <211> 930 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(907) <223> RXN02342 <400> 167 cagqttcgtg ctgcgtgcgg gtagttttta aaggtaaaac tttttgggcg tgtcgccctt aaagcgcgct tttcgacgcg accccactac attggcttcc atq aac gtt qac att 115 Met Asn Val Asp Ile 1 tca cga tcc aga gag ccg cta aac gtt gag ctc ctg aaq gaa aaa ttg 163 Ser Arq Ser Arg Giu Pro Leu Asn Val Giu Leu Leu Lys Glu Lys Leu 15 ctc caa aac ggt gac ttt ggc cag gtc att tac gaa aaa gtg aca ggc 211 wo 01/00805 WO 0100805PCT/IBOO/00926 Leu t cc Ser t 99 T rp ggC Gly ctc Leu ctc Leu gaa Giu aaa Lys aca Th r 150 cLtt Leu gac Asp ctg Leu g cg Ala gat Asp 230 att Ilie Gin act Th r a cg Th r cgc Ar9 gt t Val1 g9 Al a ggt Gly ctc Le u 135 gtt ValI cct Pro a ga Ar9 gac Asp gta Val1 215 aaa Lys cgt Arg As n aat Asn gtg Val1 ccg Pro caa Gin Ala 9CC Ala 120 t 9C Cys gtc Val1 gtt Val1 a cc Thr cag Gin 200 tgt Cys gaa Giu gt t Val1 Gi y gct Ala aaa Lys tgg Trp cta Leu gg a Gly 105 gga Gi y 99C Gly atc Ilie cct Pro aca Th r 185 t99 Trp tcc Se r ctc Leu cgc Arg Asp ga C Asp act Thr tct Ser tct Ser ctc Leu ct9 Leu atc Ile g9t Gly cat His 170 ttc Ph e cag Gin agt Ser tta Leu ga t Asp 250 Phe t tg Leu gtc Val 9CC Ala 75 at Ile gC t Ala aaa L ys ctg Leu t 99 T rp 155 gca Ala ctt Leu ggt Gly at Ile 99t Gly 235 gct Ala Gi y ct 9 Le u gag Giu 60 cct Pro ga t Asp gtc Val1 t 99 T rp gtg Val 140 99C Gly act Th r at Ile cca Pro 99c Gly 220 gaa Glu t c9 Ser Gin gca Ala 45 ttt Ph e gag Glu caa Gin atg Met ccc Pro 125 gaa G iu act Thr Lcc Ser aat Asn agt Ser 205 ca a Gin Ala 99C Gly Ile gca Ala gat Asp t cc Ser gac Asp gcg Ala gat Asp acc Th r atc Ile gca Ala 175 ctc Le u gat Asp gtt Val1 qgt Gly 9t t Tyr 99 t Gi y cat His ca a Gin Cgg Arg ttg Leu gtt Val 99c Gly agc Ser 160 ttg Le u aca Thr tgg T rp cga Arg gt C Val1 240 cac Glu tct Ser 9c9 Ala aca Th r at Ile aat Asn caa Gin ttt Phe 145 ctg Le u gaa Giu cat His ctc Leu gtg Val 225 9C 9 Ala acc Lys 99C Gly cg t Arg atc Ile 99C Gly gac Asp atc Ile 130 ga t Asp act Th r ggt Gly ctg Le u ga t Asp 210 ctt Le u act Th r ctc Thr Val His Thr Leu Asn Ala Gly 255 260 taaggtgacg gtatggggaa ttc gaa att acg cac ctt cgc ctq cag Giu Ilie Thr His Leu Ar9 Leu Gin WO 01/00805 TJLO/92 PCT[IBOO/00926 265 (210> 168 <211> 269 <212> PRT <213> Corynebacterium glutamicum (400> 168 Met Asn Val Asp Ile Ser Arg Ser Arg Giu Pro Leu Asn Val Giu Leu 1 5 10 Leu Lys Giu Lys Leu Leu Gin Asn Gly Asp Phe Gly Gin Val Ile Tyr 25 Giu Lys Val Thr Giy Ser* Thr Asn Ala Asp Leu Leu Ala Leu Ala Gly 40 Ser Gly Ala Pro Asn Trp Thr Val Lys Thr Val Giu Phe Gin Asp His 55 Ala Arg Gly Arg Leu Gly Arg Pro Trp Ser Ala Pro Giu Gly Ser Gin 70 75 Thr Ile Val Ser Val Leu Val Gin Leu Ser Ile Asp Gin Val Asp Arg 90 Ile Giy Thr Ilie Pro Leu Ala Ala Gly Leu Ala Val Met Asp Ala Leu 100 105 110 Asn Asp Leu Gly Vai Giu Gly Ala Gly Leu Lys Trp Pro Asn Asp Val 115 120 125 Gin Ile His Gly Lys Lys Leu Cys Gly Ile Leu Val Giu Ala Thr Gly 130 135 140 Phe Asp Ser Thr Pro Thr Vai Val Ile Gly Trp Gly Thr Asn le-Ser 145 150 155 160 Leu Thr Lys Giu Glu Leu Pro Val Pro His Ala Thr Ser Leu Ala Leu 165 170 175 Giu Gly Vai Glu Val Asp Arg Thr Thr Phe Leu Ile Asn Met Leu Thr 180 185 190 His Leu His Thr Arg Leu Asp Gin Trp Gin Gly Pro Ser Val Asp Trp 195 200 205 Leu Asp Asp Tyr Arg Ala Val Cys Ser Ser Ile Gly Gin Asp Val Arg 210 215 220 Val Leu Leu Pro Gly Asp Lys Glu Leu Leu Gly Glu Ala Ile Gly Val 225 230 235 240 Ala Thr Gly Gly Giu Ile Arg Val Arg Asp Ala Ser Gly Thr Val His 245 250 255 Thr Leu Asn Ala Gly Giu Ile Thr His Leu Arg Leu Gin 260 265 WO 01/00805 <210> 169 (211> 2762 (212> DNA (213> Corynebacterium glutamicum <220> <221> CDS (222> (1)..(2739) <223> RXN00563 <400> 169 PCTIIBOO/00926 ttc Phe 1 tq Trp atc Ile aag Lys gca Ala tcc Ser ct t Leu gtc Val tac Tyr gca Ala 145 atc Ile gt C Val1 att Ile gat Asp g cg Ala gtc Val aag Lys gtg Val1 cga Arg ttg Leu 100 gg t Gi y tcc Ser gct Ala gtt Val gc t Ala 180 aaa Lys ctc Le u gc t Ala g ga Gly cca Pro tcc Ser ctt Leu ggc Gly tca Ser a ag Lys gca Ala cgc Arg 165 gaa Glu ctg Leu ta c Tyr aac As n tcc Se r gct Ala gy a Gly 70 Ctg Leu tcc Ser cca Pro gca Ala t 99 Trp 150 ggC Gly gaa Glu ctg Leu gca Ala ttg Leu gag Glu ttg Leu t CC Ser ctc Leu tc Ser aac Asn gct Ala 135 gga Gly acc Thr gca Al a tcc Se r cgc Arg t ca Se r acc Th r ttg Leu gca Ala gag Glu gg t Gly ttc Phe gtg Val1 tcc Ser 155 9gC Gly acc Th r tc Se r 99C Gly ga c Asp gtC Val ctg Le u ggc Gly CgC Arg cac His ggt Gly gtt Val1 140 CtC Le u ggC Gly tac T yr act Th r 48 96 144 192 240 288 336 384 432 480 528 576 624 WO 01/00805 WO 0100805PCTJIBOO/00926 gca ged Ala Ala 210 tcc gcg cca atc Ser Ala Pro Ile gtc gac ttc act Val Asp Phe Thr gga ctt ggt gaa Gly Leu Gly Glu gat etg aac ctg Asp Leu Asn Leu gcg Ala 230 gaa atg gca cgt Glu Met Ala Arg gaa gca gct aag Glu Ala Ala Lys get aac gca eca Ala Asn Ala Pro gtt gag gct cca Val Giu Ala Pro aca gtg gca gca Thr Val Ala Ala etg cca Leu Pro 255 act cct tac Thr Pro Tyr gte ace eaa Val Thr Gin 275 eca gtg gtt caa Pro Val Val Gln ace cct gat ttc Thr Pro Asp Phe gca ggt caa Ala Gly Gin 270 gee gge gag Ala Gly Glu aae ctt gac gag Asn Leu Asp Giu gte gte ate gtt Val Val Ile Val etc gge Leu Gly 290 eca ctg ggt tet Pro Leu Gly Ser gea Al a 295 egt aeg egt tte Arg Thr Arg Phe gee gaa etc aae Aia Glu Leu Asn gg t Gi y 305 tee etc tee gee Ser Leu Ser Ala ggt gte ate gaa Gly Val Ile Glu gea tgg aeg atg Ala Trp Thr Met ett ate eac tgg Leu Ilie His Trp gaa gat eca aag Glu Asp Pro Lys gge tgg tac gae Gly Trp Tyr Asp gac tee Asp Ser 335 gac gac gea Asp Asp Ala gte atg gca Val Met Ala 355 gee gaa gaa gae Ala Glu Glu Asp tte gac ege tac Phe Asp Arg Tyr cac gac gaa His Asp Glu 350 cet gag tac Pro Glu Tyr ege gtt ggt gte Arg Val Gly Val aag tae aat gac Lys Tyr Asn Asp 912 960 1008 1056 1104 1152 1200 1248 1296 1344 ggc atg Gly Met 370 ate gac aae ttt Ile Asp Asn Phe g ca Ala 375 eca gag etg ace Pro Glu Leu Thr gte tac etc gac Vai Tyr Leu Asp cag Gin 385 gac etc ace tte Asp Leu Thr Phe gtg gga tee ege Val Gly Ser Arg gag gea etg ace Glu Ala Leu Thr gte gac tee gag Val Asp Ser Glu gaa etc ace ttt Giu Leu Thr Phe tet tte gac gaa Ser Phe Asp Glu gca gea Ala Ala 415 ggg gag tgg Gly Glu Trp ege ege atg Arg Arg Met 435 gte act ege aag Val Thr Arg Lys gge tee gca ate ege gta ect Gly Ser Ala Ile Arg Vai Pro 430 geg atg ace ege Ala Met Thr Arg gtt ggt gga eag Val Gly Gly Gin cet aag gac Pro Lys Asp WO 01/00805 PCTJIBOO/00926 ttc gac cca gct gtg tgg ggc att cca gct gac atg gtg gac aac ctg 1392 Phe Asp Pro Ala Val Trp Gly Ile Pro Ala Asp Met Val Asp Asn Leu 450 455 460 gac acc gtc gcg ctg tgg aac att gtc tgt act gtc gac gcc ttc ctg 1440 Asp Thr Val Ala Leu Trp Asn Ile Val Cys Thr Val Asp Ala Phe Leu 465 470 475 480 tcc gct gga ttc acc cca gca gag ctg ctt gct tcc gtt cac cca gca 1488 Ser Ala Gly Phe Thr Pro Ala Giu Leu Leu Ala Ser Val His Pro Ala 485 490 495 cgc gtg tcc tct acc caa ggc acc gqc atg ggc ggc atqgqaa tcc ctc 1536 Arg Val Ser Ser Thr Gin Gly Thr Gly Met Gly Gly Met Giu Ser Leu 500 505 510 cgt ggc atc tac gtc gac cgc att ctg gca gag cca cgc gcc aac gac 1584 Arg Gly Ile Tyr Val Asp Arg Ile Leu Ala Giu Pro Arg Ala Asn Asp 515 520 525 gtt ctg cag gaa gca ctg ccc aac gtt gtt gca gct cac gtc atq cag 1632 Val Leu Gin Glu Ala Leu Pro Asn Val Val Ala Ala His Val Met Gin 530 535 540 tcc tac gtc ggt ggc tac-gga cag atg atc cac cca gtc gca gct tgt 1680 Ser Tyr Val Gly Gly Tyr Gly Gin Met Ile His Pro Val Ala Ala Cys 545 550 555 560 gca acc gca gct gtt tct gtg gaa gaa gca ctg gac aag atc cgc atc 1728 Ala Thr Ala Ala Val Ser Val Giu Giu Ala Leu Asp Lys Ile Arg Ile 565 570 575 ggc aag tcc gac ttc gtt gtc gca ggt ggc ttc gat gcc ctg tcc gtt 1776 Gly Lys Ser Asp Phe Val Val Ala Gly Gly Phe Asp Ala Leu Ser Val 580 585 590 gaa ggc atc acc ggc ttc ggc gac atg gca gca acc gcc gac tcc gca 1824 Glu Gly Ile Thr Gly Phe Gly Asp Met Ala Ala Thr Ala Asp Ser Ala 595 600 605 gag atg gaa ggc aag gga att gag cac cgc ttc ttc tcc cgc gcc aac 1872 Glu Met Giu Gly Lys Gly Ile Giu His Arg Phe Phe Ser Arg Ala Asn 610 615 620 gac cgg cgc cgc ggt gga ttc atc gaa tcc gaa ggt ggc gga acc gtc 1920 Asp Arg Arg Arg Gly Gly Phe Ile Glu Ser Giu Gly Gly Gly Thr Val 625 630 635 640 ctt ctg gca cgc gga tca ctc gca gct gac ctg ggc ctt cca gta ctc 1968 Leu Leu Ala Arg Gly Ser Leu Ala Ala Asp Leu Gly Leu Pro Val Leu 645 650 655 ggt gtc atc gga ttc gca gag tcc ttt gca gat ggt gcc cac acc tcc 2016 Gly Val Ile Gly Phe Ala Glu Ser Phe Ala Asp Gly Ala His Thr Ser 660 665 670 atc cca gcc cca ggc ctc ggt gcc ctt ggt gct gct cgc gat ggt gtg 2064 Ile Pro Ala Pro Gly Leu Gly Ala Leu Gly Ala Ala Arg Asp Gly Val 675 680 685 WO 01/00805 WO 0100805PCTIBOO/00926 gaa Glu gag Giu 705 cca Pro gca Ala cac His gtc Val gac Asp 785 c ta Leu 99C Gly ttc P he t gg T rp 9 tc Val1 865 aac As n ctc Leu cgc Arg tcc Ser gag Glu 99c Gly aag Lys 755 cga Arg gta Val1 ctt Leu g ga Gly gag Giu 835 gca Ala 9gt Gly 99t Gly ga t Asp ctt gca gta Leu att Ile tcc Ser aa c Asn 740 gg t Gly tcc Ser ctg Leu cgt Ar g cac His 820 gca Ala tcc Ser atg Met gtc ValI t cc Ser 900 Ala atc Ile ga c Asp 725 ccg Pro gg t Gi y gga Gly tcc Ser gcg Ala 805 gtc Val1 gtt Val1 g cg Ala cac His cac H is 885 cgc gca Ala 695 aag Lys ca c His tac Tyr gca Ala gtg ValI 775 cat His gca Ala gct Al a gtg Val1 gct Ala 855 gg C Gi y ga c Asp cgc gtC Val1 t cc Ser 715 g ca Ala cag Gin atg Met cg C Arg gt C Val1 795 99 t Gly att Ile gc t Al a g ca Ala ga a Glu 875 gaa Gi u gg t Gly 700 acc Thr tcc Se r aag Ly s atc Ile gca Ala 780 tgg Trp ctt Leu gt t Val1 gag Glu ggc Gi y 860 cgc Arg gtt ValI gtc Val1 aac Asn gct Ala tca Ser ggt Gi y 765 ctc Le u ctg Leu gtt Val1 cac His gca Ala 845 ctt Le u cca Pro gaa Glu t ct Ser gcg Ala atc Ile ctc Leu 750 ctc Leu gac Asp cgc Arg acc Thr cca Pro 830 gct Ala cgt Arg gtc Val1 gct Ala gCt Ala aat Asn gg t Gly 735 acc Th r acc Th r tgc Cys aag Lys t CC Se r 815 gac Asp gac Asp acc Thr gag Glu gca Ala 895 ga t Asp gat Asp 720 cgt Arg gga Gly ca g Gin gt t Val cca Pro 800 ctt Leu gcc Ala gta Val1 atc Ile cg c Arg 880 gt c Val1 ga a Glu 2112 2160 2208 2256 2304 2352 2400 2448 2496 2544 2592 2640 2688 2736 2762 gtt gac ggt gtc Arg Ala Arg Leu Val Asp 905 Gly Val ctc cgc gcc Leu Arg Ala 910 tagttggtta ttgcgttgag ccc WO 01/00805 <210> 170 <211> 913 <212> PRT <213> Corynebacterium giutamicum (400> 170 Phe Tyr Lys Asp Leu Tyr Ala Arg Ser PCT[IBOO/00926 1 Trp Ile Lys Ala Ser Leu Val1 Tyr ,Ala 145 Ile Val1 Ile Ala Ser 225 Ala Th r Val Ile Asn Leu Pro Gin Aila Ile Giy 130 Giu Giy Lys Al a Ala 210 Asp As n Pro Thr Val1 Trp Val Arg Met Pro Pro 115 Glu Gin T rp Al a Glu 195 Se r Leu Al a Tyr Gin 275 Ala Val1 Lys Val1 Arg Leu 100 Giy S er Aia Val1 Ala 180 Lys Ala Asn Pro Arg 260 As n Asn S er Ala Gly 70 Leu Ser Pro Ala T rp 150 Gly Giu Le u Ile Ala 230 Val1 Val1 Asp Leu Giu Le u 55 Ser Leu Ser As n Ala 135 Gly Thr Ala Ser Thr 215 Glu Glu Val1 Glu Ser Gin 40 Val1 Met T rp Ile Arg 120 Leu Al a Gly Giy Gin 200 Val1 Met Ala Gin Met 280 Ser Thr Pro Ala Se r As n 105 Gly Asp His Leu Val1 185 Al a Asp Ala Pro Thr 265 Val1 Ala 10 Tyr T hr Th r Asp Val Val1 Arg Ala Thr Met 170 Giu Thr Phe Arg Arg 250 Thr Val Arg Arg Se r Th r Le u Ala 75 Gi u Gi y Ph e Val1 Se r 155 Gi y Thr Se r Th r Ala 235 Thr Pro Ile Ph e Gly Thr Asp Ile Val Asn Leu Phe Gly Pro Arg Leu His Arg Gly Gly 125 Val Thr 140 Leu Val Gly Asn Tyr Ser Thr Val 205 Gly Gly 220 Glu Ala Val Ala Asp Phe Val Gly 285 Asp Ala Ala Ala Ala Phe Ala Al a His Gly Trp Ala Pro 175 Gin Glu Gly Lys Leu 255 Gly Gi y Le u Le u Ile Ser Al a Glu Gly Val1 Ala As n His 160 Leu Glu Gin Glu Ala 240 Pro Gin Glu Asn Leu Gly Pro Leu Gly Ser Ala Arg Thr WO 01/00805 WO 0100805PCTIBOOIOO926 Gly 305 Le u Asp Va 1 Gly Gin 385 Val Gi y Arg Ph e Asp 465 S er Arg Arg Val Ser 545 Ala Gly Giu Giu Ser T rp Val 340 Arg Asp Th r Giu Ly s 420 Ala Al a Al a Ph e Ser 500 Tyr Glu Gi y Al a Asp 580 Th r Gly Ala Asp 325 Ala Val Asn Phe Pro 405 Val Met Val Leu Th r 485 Thr Va i Ala Gi y Val 565 Phe Gly Lys Ala 310 Glu Glu Gly Phe As n 390 Glu Thr Thr Trp Trp 470 Pro Gin Asp Le u Tyr 550 Ser Val1 Ph e Gly Ile Glu Lys Pro 330 Ile Phe 345 Lys Tyr Glu Leu Ser Arg Phe Ala 410 Ala Gly 425 Val Gly Pro Ala Val Cys Leu Leu 490 Gly Met 505 Leu Ala Vai Val Met Ile Giu Ala 570 Gly Gly 585 Met Ala His Arg Leu 315 Giy Asp Asn Thr Giu 395 Ser Ser Gly Asp Thr 475 Ala Gl y Glu Ala His 555 Leu Ph e Ala Phe Aia Trp Arg Asp Thr 380 Glu Ph e Ala Gin Met 460 Val1 Ser Giy Pro Aila 540 Pro Asp Asp Th r Phe 620 T rp Tyr Tyr Met 365 Val Ala Asp Ile Val 445 Val1 Asp Val1 Met Ar g 525 His ValI Lys Ala Ala 605 Ser Thr Asp His 350 Pro Tyr Le u Glu Arg 430 Pro Asp Ala His Giu 510 Al a Val1 Ala Ile Le u 590 Asp Arg Met Asp 335 Asp Giu Leu Thr Ala 415 Val1 Lys Asn Phe Pro 495 Ser Asn Met Ala Arg 575 Ser Se r Ala Gly 320 Ser Glu Tyr Asp Tyr 400 Ala Pro Asp Leu Leu 480 Ala Leu Asp Gin Cys 560 Ile Val1 Al a As n WO 01/00805 WO 0100805PCT[IBOO/00926 Asp 625 Leu Gly Ile Gi u Glu 705 Pro Ala His Val Asp 785 Le u Gly Ph e Trp Val1 865 Asn Arg Leu Val Pro Ser 690 Ile Asn Asp Ala Le u 770 Pro Asp Ph e T yr Arg 850 Ala Le u Arg Arg Ala Arg Ile Gly 660 Ala Pro 675 Arg Leu Ser Ile Glu Ser Gly Asn 740 Lys Gly 755 Arg Ser Val Leu Leu Arg Gly His 820 Glu Ala 835 Ala Ser Gly Met Gly Val Gly Gi y 645 Phe Gly Ala Ile Asp 725 Pro Gly Gly Ser Ala 805 Val1 Val1 Ala His His 885 Gly 630 Ser Ala Leu Val1 Ser 710 Leu Met Ala Leu Lys 790 L ys Ser Arg Ile Gly 870 Gly Ph e Leu Glu Gly Al a 695 Lys His Tyr Al a Val1 775 His Ala Ala Val1 Ala 855 Gi y Asp Ilie Ala Se r Ala 680 Leu His Glu Val1 Ala 760 Pro Se r Pro Le u Ala 840 Arg Val1 Ala Glu Ala Phe 665 Leu Arg Asp Arg Ile '74 5 Phe Ala His Lys Val 825 Arg Glu Le u Ala Ser Asp 650 Ala Gly Ser Thr Ile 730 Ser Gin Asn Le u Al a 810 Ala Gly Glu T yr Lys 890 Glu Gly 635 Leu Gly Asp Gly Ala Ala Val Gly 700 Ser Thr 715 Ala Ser Gin Lys Met Ile Arg Ala 780 Val Trp 795 Gly Leu Ile Val Ala Glu Ala Gly 860 Glu Arg 875 Glu Val Gly Leu Al a Arg 685 Val1 As n Ala Ser Gly 765 Leu Leu Val His Ala 845 Leu Pro Glu Gly Pro His 670 Asp Ser Ala Ile Leu 750 Leu Asp Arg Thr Pro 830 Ala Arg Val1 Ala Th r Val1 655 Thr Gly Ala As n Gly 735 Thr Thr Cys Lys Ser 815 Asp Asp Thr Glu Ala 895 ValI 640 Le u Ser ValI Asp Asp 720 Arg Gly Gln Val1 Pro 800 Le u Ala Val1 Ile Arg 880 Val1 Leu Leu Asp Ser Arg Ala Arg Leu Val Asp Gly Val Leu 900 905 GI y <210> 171 (211> 2937 Arg 910 Ala Glu WO 01/00805 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (2914) <223> RXN02168 <400> 171 agggtttagt catctatttc ggctacgctc acgtaaccta PCTLBOOIOO926 cgcattcgta agtggtcatg ggtccaagaa gcccattcca agacttagga gctttatttc gtg agt att tct tca Val Ser Ile Ser Ser ctg Leu cag Gin atc Ile act Thr gct Aia atc Ilie atc Ile gct Aia gac Asp gct Ala 150 ctg Leu gag Giu aca Thr gct Ala acc Thr gca Al a cg t Arg gac Asp gc t Ala t cc Ser aat As n 135 t cc Se r tcc Se r gt c Val ccg Pro tct Ser gca Ala cc t Pro ctt Leu gca Aia gca Ala cgc Arg 120 gaa Giu cag Gln cg t Arg t cc Ser ctg Leu gcc Ala acg Thr ttc Ph e gag Glu ca g Gin acc Thr 105 ctt Le u ga a Giu ttc Phe gag Glu ctg Le u tct Ser cag Gin ctg Le u cgc Arg ctg Leu 75 gc c Ala cag Gin ca c His gt t Val1 Gly 155 atc Ile aac As n ttc Phe cag Gin cgc Arg caa Gin 60 acc Thr gtt Val1 ctg Leu tcc S er t ta Leu 140 aag Lys cag Gin .gc t Al a aaa Lys gtg Val1 gag Giu 45 atc Ile cgc Arg tcc Se r cg t Arg cag Gin 125 gct Ala ggc Gly gac Asp cgt Arg gag Glu atc Ile 30 ctc Leu acc Th r gaa Glu att Ile ga c Asp 110 ggc Gi y ttc Phe gca Al a acc Th r gca Al a gca Al a gat Asp tct Se r atc Ile g ca Al a 80 ggc Gi y gga Gly ttg Le u atc Ile atg Met 160 gct Ala ttt Phe 1 att Ile tcc Ser cgc Arg gtg Val1 caa Gin att Ile ctc Leu ggC Gly ctg Leu 145 ctc Leu gg t Gly gtt Val1 ctg Leu agc Ser tcc Ser CC t Pro atc Ile ctg Leu ga t Asp gt t Val1 130 Ctg Leu tct Ser gtc Val gt C Val tac Tyr gaa Glu cot Arg ggc Gly gg t Gly ctg Leu gtc Val 115 gaa Glu gg C Gly gtt Vali ga t Asp tct Se r gcc Ala gac Asp g ca Ala tca Ser gca Ala gga Gly 100 gca Ala gca Ala gca Ala cgc Arg ggg Gly 180 ggt Gly gg t Gi y cac His aag Lys ct t Leu ga c Asp cag Gin gca Al a gta Val gca Ala gg c Gly 165 gta Val aag Ly s 115 163 211 259 307 355 403 451 499 547 595 643 691 WO 01/00805 WO 0100805PCT/IBOO/00926 cca Pro gitt Val1 gag Glu 230 t cc Ser ggc Gi y cc a Pro ctt Leu gg i Gly 310 aac Asn gag Glu cic Leu 9gc Gi y aac As n 390 gaa Glu aag Lys gac Asp ga c Asp gca Al a 250 aac As n gt Val1 gca Ala tci Ser cct Pro 330 aag Lys icc Se r acc Thr t gg Trp aag Lys 410 aag Lys aic Ile tac Tyr 235 gcc Ala gcg Ala gag Glu ggc Gly t ig Leu 315 ggc Gly ctg Leu cgc Arg acc Th r gcc Al a 395 aac Asn gci Ala aac As n 220 tig Leu ga c Asp cg c Arg cag Gln gtc Val 300 gt Val ttc Ph e gitt Val cig Leu gt ValI 380 ga a Glu a ag Lys gca Ala tcc Ser cac His acc Thr cta L eu 275 gat Asp i ig Leu gag G lu gati Asp cac His 355 gtc Val gct Al a tac Tyr ctc Le u gct Ala cig Leu cac His acc Thr 260 gt ValI tac Tyr cta Leu cgc Arg t gg T rp 340 aag Lys cig Leu gca Ala tci Se r aag Lys 420 gati Asp gca Ala icc Ser 245 tgt Cys aac As n gt Val gac Asp gac Asp 325 icc Se r gtt Val1 gct Ala gcg Al a ga a Glu 405 gt t Val1 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 gga cgc icc gca Gly Arg Ser Ala 425 cag tic aac icc Gln Phe Asn Ser atg tic tic gac Met Phe Phe Asp 430 cgc tac aig igg Arg Tyr Met Trp 435 wo 01/00805 WO 0100805PCTIBOOIOO926 aac As n gg t Gly gag Giu 470 tac Tyr aaq Lys ga c Asp ctc Le u atg Met 550 aCC Thr ggc Gly tct Ser cac His a cc T hr 630 ga t Asp ctg Le u acc Thr 455 gaa Gi u gtt Val1 att Ile gga Gly ctg Leu 535 atc Ile ggt Gly atc Ile cc t Pro gac Asp 615 tct Ser tct Se r cag Gin 440 tcc Ser q ca Ala g ca Aia gc t Aia cac His 520 acc Th r ggt Gly ga a Giu ct C Leu ca g Gin 600 qct Aia ggt Giy gct Ala ttc ggt Phe Giy atc aac Ile Asn act gag Thr Giu ttc aag Phe Lys 490 gat gca Asp Ala 505 gct ggt Ala Gly acc tac Thr Tyr ggc ggc Gly Gly tgg tcc Trp Ser 570 gtg ggt Val Gly 585 gtc aag Val Lys Gly Giy ctg tca Leu Ser gca gct Ala Ala 650 gca cag cgc atc gtt tcc aag gca cgt Ala Gin Arg Ile Val Ser Lys Ala Arg gca acc Ala Thr 445 ggt gtt gtt Giy Val Val 460 ctg atc aac Leu Ile Asn 475 cca ggc acc Pro Gly Thr aac cca gag Asn Pro Giu ggc cac cac Gly His His 525 gca gag ctg Ala Giu Leu 540 atc gga acc Ile Gly Thr 555 acc gat ttg Thr Asp Leu acc gct gcc Thr Ala Ala cag gca ctg Gin Ala Leu 605 tgg gtt ggc Trp Val Gly 620 cac ctg cac His Leu His 635 tcc cgc ctg Ser Arg Leu 450 gtc Val gat Asp gtg Val1 acc Thr 510 tcc Ser cg t Arg cc t Pro ggc Giy atg Met 590 gt C Val cg t Arg gct Al a atc Ile tcc gct Ser Ala ctg aac Leu Asn 480 gat cag Asp Gin 495 aag atc Lys Ile tgq gtc Trp Val tcc cgc Ser Arg gca aag Ala Lys 560 ttc cca Phe Pro 575 gca acc Ala Thr gac acc Asp Thr ggc gat Gly Asp gac atg Asp Met 640 tct. tcc Ser Ser 655 gag gct Glu Ala gag Giu ttc Phe aCC Thr 500 atc Ile gat Asp gtc Val1 ta C Tyr gtg Val1 580 acc Th r ga t Asp ggC Gly gac Asp ga t Asp 660 gtt Val cca Pro 485 ctg Leu gag Giu ctg Leu gtc Val1 ctg Leu 565 ga c Asp act Thr cca Pro gtg Val1 aac Asn 645 ga t Asp gct Ala 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 tac gca Tyr Ala gat cac cgc gaa. gag ctc atc Asp His Arg Glu Giu Leu Ile 665 670 atc aac aag acc Ile Asn Lys Thr 675 WO 01/00805 aag cct ttc ttc ggc gag gtc gaa gag PCTIBOO/00926 L ys cag Gin gat Asp 710 g ca Ala gaa Glu gcc Ala Ctg Leu gc t Ala 790 aac Asn t cc Ser ggc Gly gag Gi u ctg Le u 870 gct Al a ta c Tyr gtt Pro cg t Arg 695 cg t Arg gag GI u aat As n cgc Arg t gc Cys 775 ga c Asp gag Glu gtc ValI cgc Arg atc Ile 855 gct Ala cac His t gg T rp gag Ph e gtt Val ctc Le u ggc Gly gaa Glu 745 atc Ile aag Lys qca Ala tac Tyr ggc Gly 825 gaa Glu gc t Ala cca Pro ctc Leu atc Ile 905 cgc Gly gag Glu ga t Asp gcc Al a 730 gag Glu cag Gin cac His cgc Ar g ggc Gi y 810 atc Ile gct Al a cg c Arg cac His aac As n 890 cg t Arg cca Glu ct t Leu t tg Leu 715 atc Ile gcc Ala gtc Val1 cac His tgq T rp 795 gcg Al a ga c Asp gcc Ala ctc Leu atc Ile 875 gag Glu atc Ile ta c Val1 gct Ala 700 gtt Val1 acc Thr gtc Val1 tct Ser aag Lys 780 tgg Trp aac Asn cg t Arg tgc Cys aat As n 860 gtg Val1 gg t Gly ctg Le u ct g Glu 685 tac Tyr cac His aca Thr gja a Glu gcg Ala 765 cct Pro gg c Gl y t ca Ser gtt Val1 gtt Val1 845 gag Giu tgg T rp gct Al a gct Ala gt t G li c ca Pro cgc A rg ctg Leu aag Lys 750 cgc Arg atg Met ctt Le u gtc Val1 gac Asp 830 ga c Asp tcc Ser cac His ttc Phe ga t Asp 910 cag atg Met act Th r att Ile ttc Phe 735 ctt Le u ga c Asp cc t Pro gac Asp cgc Arg 815 gag Glu gc t Ala a ag Lys ggc Gly gag Glu 895 tcc Se r cat act tac gca Thr Tyr Ala 690 cag gac cca Gin Asp Pro 705 gaa gct cgt Glu Ala Arg 720 cca gac cat Pro Asp His ctt gct gct Leu Ala Ala gcc gcg tgg Ala Ala Trp 770 tgg gtt cca Trp Val Pro 785 acc ctg tgg Thr Leu Trp 800 gtt atc cca Val Ile Pro cca gtt gca Pro Val Ala ctc gac ggc Leu Asp Gly 850 aac gag cgc Asn Glu Arg 865 aac ctg atc Asn Leu Ile 880 ctc atc gag Leu Ile Glu tac ttc gac Tyr Phe Asp gtt gac atc gag tgg atc Glu Trp Ile acc tgg gat Thr Trp Asp ctc aac gag Leu Asn Glu 725 gcg tct gtg Ala Ser Val 740 tac ccg cag Tyr Pro Gin 755 ttt att ggt Phe Ile Gly gca atc gat Ala Ile Asp cag tcc cag Gin Ser Gin 805 gga cca gtc Gly Pro Val 820 gag ctg ctc Glu Leu Leu 835 gag cca gaa Glu Pro Glu gaa ttc ctg Glu Phe Leu gac aac cca Asp Asn Pro 885 gag gat ggc Glu Asp Gly 900 gat ctg cca Asp Leu Pro 915 cca gtt gag 2179 2227 2275 2323 2371 2419 2467 2515 2563 2611 2659 2707 2755 2803 2851 2899 WO 01/00805 PCT/IB00/00926 Val Glu Gin Arg Pro Tyr Leu Val Gin His Val Asp Ile Pro Val Glu 920 925 930 ctg ggt gac gct ggt tgaaccggtg gtttcccaat tgg 2937 Leu Gly Asp Ala Gly 935 <210> 172 <211> 938 <212> PRT <213> Corynebacterium glutamicum <400> 172 Val Ser Ile Ser Ser Leu Thr Pro Leu His Ser Phe Lys Glu Pro Ala 1 5 10 Ile Leu Tyr Ala Gly Gin Ala Ser Ala Trp Gin Gin Val Ile Ala Asp 25 Ser Ser Glu Asp His Ile Thr Ala Thr His Leu Arg Glu Leu Leu Ser 40 Arg Ser Arg Ala Lys Thr Ala Pro Phe Ala Arg Gin Ile Thr Ala Ile 55 Val Pro Gly Ser Leu Ala Arg Leu Glu Glu Leu Thr Arg Glu Asp Ala 70 75 Gin Ile Gly Ala Asp Ile Asp Ala Gin Pro Ala Val Ser Ile Pro Gly 90 Ile Leu Leu Gly Gln Ile Ala Ala Thr Arg Gin Leu Arg Asp Leu Gly 100 105 110 Leu Asp Val Ala Ala Ala Ser Arg Leu Gly His Ser Gin Gly Ile Leu 115 120 125 Gly Val Glu Ala Val Asp Asn Glu Glu Asp Val Leu Ala Phe Ala Ile 130 135 140 Leu Leu Gly Ala Ala Ala Ser Gin Phe Ala Gly Lys Gly Ala His Met 145 150 155 160 Leu Ser Val Arg Gly Leu Ser Arg Glu Ile Ile Gin Asp Thr Ile Ala 165 170 175 Gly Val Asp Gly Val Glu Val Ser Leu Arg Asn Ala Arg Ala His Phe 180 185 190 Val Val Ser Gly Lys Pro Glu Ala Leu Lys Lys Ala Ala Ala Ala Leu 195 200 205 Gin Arg Ala Ala Asp Val Tyr Asn Glu Asp Ile Asn Glu Lys Arg Lys 210 215 220 Gly Gly Ser Leu Ala Glu Pro Lys Phe Asp Tyr Leu Asp Val Ala Ile 225 230 235 240 Pro Phe His His Ser Ser Met Gin Asp Ala Ala Asp Leu Ala Val Glu 245 250 255 WO 01/00805 WO 0100805PCTAIBOO/00926 Trp Ala Lys Ala 305 Ala Ala Gly Pro Val 385 Gly Ser Asp Lys Gly 465 Al a Ile Ile As n Lys 545 Al a Thr 260 Val1 Tyr Leu Arg Trp 340 Lys Le u Ala Ser Lys 420 Met Ala Glu Phe Th r 500 Ile Asp Val Tyr C ys Asn Val1 Asp Asp 325 Ser Val1 Ala Ala Glu 405 Val1 Trp Thr Val Pro 485 Le u Glu Le u Val Le u 565 Arg Gin Val1 300 Val Phe Val1 Le u Val1 380 Glu Ly s As n Gln Val1 460 Ile Gly Pro His Glu 540 Gly Asp Ala Ile 285 Ser Pro His Lys Thr 365 Asp Met Giu Se r Arg 445 Val Asn Th r Glu His 525 Le u Thr Leu WO 01/00805 PCT/IB00/00926 Ala Met Pro Val Asp Gly Ile Leu Val Gly Thr Ala Ala Met Ala Thr 580 585 590 Lys Glu Ala Thr Thr Ser Pro Gin Val Lys Gin Ala Leu Val Asp Thr 595 600 605 Pro Gly Val Asp Pro His Asp Ala Gly Gly Trp Val Gly Arg Gly Asp 610 615 620 Ala Arg Gly Gly Val Thr Ser Gly Leu Ser His Leu His Ala Asp Met 625 630 635 640 Tyr Glu Leu Asp Asn Asp Ser Ala Ala Ala Ser Arg Leu Ile Ser Ser 645 650 655 Ile Asp Ser Asp Asp Tyr Ala Asp His Arg Glu Glu Leu Ile Glu Ala 660 665 670 Ile Asn Lys Thr Ala Lys Pro Phe Phe Gly Glu Val Glu Glu Met Thr 675 680 685 Tyr Ala Glu Trp Ile Gin Arg Trp Val Glu Leu Ala Tyr Pro Thr Gin 690 695 700 Asp Pro Thr Trp Asp Asp Arg Phe Leu Asp Leu Val His Arg Ile Glu 705 710 715 720 Ala Arg Leu Asn Glu Ala Glu His Gly Ala Ile Thr Thr Leu Phe Pro 725 730 735 Asp His Ala Ser Val Glu Asn Glu Glu Glu Ala Val Glu Lys Leu Leu 740 745 750 Ala Ala Tyr Pro Gin Ala Arg Glu Ile Gin Val Ser Ala Arg Asp Ala 755 760 765 Ala Trp Phe Ile Gly Leu Cys Arg Lys His His Lys Pro Met Pro Trp 770 775 780 Val Pro Ala Ile Asp Ala Asp Leu Ala Arg Trp Trp Gly Leu Asp Thr 785 790 795 800 Leu Trp Gin Ser Gin Asn Glu Arg Tyr Gly Ala Asn Ser Val Arg Val 805 810 815 Ile Pro Gly Pro Val Ser Val Ala Gly Ile Asp Arg Val Asp Glu Pro 820 825 830 Val Ala Glu Leu Leu Gly Arg Phe Glu Ala Ala Cys Val Asp Ala Leu 835 840 845 Asp Gly Glu Pro Glu Glu Ile Phe Ala Arg Leu Asn Glu Ser Lys Asn 850 855 860 Glu Arg Glu Phe Leu Leu Ala Thr Pro His Ile Val Trp His Gly Asn 865 870 875 880 Leu Ile Asp Asn Pro Ala His Val Leu Asn Glu Gly Ala Phe Glu Leu 885 890 895 Ile Glu Glu Asp Gly Tyr Trp Val Ile Arg Ile Leu Ala Asp Ser Tyr WO 01/00805 PCT[IBOO/00926 900 905 910 Phe Asp Asp Leu Pro Vai Giu Gin Arg Pro Tyr Leu Val Gin His Vai 915 920 925 Asp Ile Pro Val Giu Leu Giy Asp Ala Giy 930 935 <210> 173 <211> 921 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <222> (898) <223> RXN01090 <400> 173 gccggiiigg gciggitgga gctctagatc giaagiggtg tcgiacccat gacgiaccat taaccacgaa cgiittaaag aagccacgaa ggagcctgac atg gcg tta cca cta Met Ala Leu Pro Leu ccc Pro gg c Gly tt g Leu gag Giu gati Asp atc Ilie aag Lys a ca Th r agc Se r ctc Le u tt Val1 aag Lys gag Giu aac As n ga c Asp gcc Ala aag Lys gcc Al a gct Ala aag Lys cca Pro aic Ilie cag Gin gt Vai 120 gci Al a gcc Ala cgc Arg ggc Gi y cgc Arg at i Ile tgg Trp gag Glu cga Arg aaa Lys gag Giu gtg Val1 a ag Lys 75 aac Asn tct Ser cic Le u act Thr 15 cgg Arg aaa Lys gtc Val1 gati Asp atc Ile 95 gc C Al a gig Val g gg Giy tat T yr gag Giu cgc Arg gag Giu 80 gca Ala cag Gin tig Le u 1 gca Al a ggg Gly aic Ile ccg Pro atc Ile agc Se r ttc Phe gg i Gi y agc Ser cac His gcc Ala gt g Val1 aag Ly s ttt Phe t ca Ser g gc Gly 130 caa Gin aac Asn gca Al a gat Asp aca Thr ggg Gly ct Leu 115 aig Met ggc Gly ctc Leu gat Asp tg Leu agg Arg ccg Pro 100 aat Asn att Ile at Ile ati Ile cia Le u agt S er gaa Giu tic Phe gcc Ala gac Asp 115 163 211 259 307 355 403 451 499 547 cgi ggc acg Arg Gly Thr 135 gct ati tgc aai gig gga ici gcg gct ggc aai gtg Ala Ilie Cys Asn Val Gly Ser Ala Ala Gly Asn Val 140 145 WO 01/00805 PTIO/02 PCT/IBOO/00926 cca atc ccc aac aac gcc acg tat gtg ctc acc aag gct ggc gtg aac 595 Pro Ile Pro Asn Asn Ala Thr Tyr Val Leu Thr Lys Ala Gly Val Asn 150 155 160 165 gcg ttc acc gag gca atg cat tat gag ctg cgc gga act ggt gtg gcg 643 Ala Phe Thr Giu Ala Met His Tyr Glu Leu Arg Gly Thr Gly Val Ala 170 175 180 tgt act ttg ctc gca ccg gga cct gtc cgt gag gcg gag atc cct gag 691 Cys Thr Leu Leu Ala Pro Gly Pro Val Arg Glu Ala Glu Ile Pro Glu 185 190 195 tct gag aag tcg atc gtg gac aag gtt gtc cct gat ttc ttg tgg acc 739 Ser Glu Lys Ser Ile Val Asp Lys Val Vai Pro Asp Phe Leu Trp Thr 200 205 210 acc tat gag tcc tgc tcc gca gag acc ttg cgt gcg ctg tct aag aat 787 Thr Tyr Glu Ser Cys Ser Ala Giu Thr Leu Arg Ala Leu Ser Lys Asn 215 220 225 cag cgt cgc gtt gtt cca ggt ccg ctg tcc aag gcc atg aat ttt gtg 835 Gin Arg Arg Val Vai Pro Gly Pro Leu Ser Lys Ala Met Asn Phe Val 230 235 240 245 tcc tct gtt gct cca acc gct gtg ctc tcc cct gtt atg ggc tgg gtt 883 Ser Ser Val Ala Pro Thr Ala Val Leu Ser Pro Val Met Gly Trp Val 250 255 260 tat aag aag atg ggt tagtttttaa aagtgtctga atc 921 Tyr Lys Lys Met Gly 265 <210> 174 <211> 266 <212> PRT <213> Corynebacterium glutamicum <400> 174 Met Ala Leu Pro Leu Pro Ser Lys Ser Ala Arg Ala Leu Val Thr Gly 1 5 10 Ala Ser Gin Gly Ile Giy Leu Ala Ile Ala Lys Asp Leu Ala Arg Tyr 25 Gly His Asn Leu Ile Leu Val Ala Arg Arg Glu Asp Val Leu Lys Glu 40 Ile Ala Ala Asp Leu Glu Lys Lys His Gly Val Ilie Val Glu Val Arg 55 Pro Val Asp Leu Ser Asp Giu Pro Ala Arg Lys Val Leu Ile Asp Giu 70 75 Ile Lys Thr Arg Glu Ile Asn Ile Ile Ile Asn Ser Ala Gly Ile Ala 90 Ser Phe Gly Pro Phe Lys Asp Gin Asp Trp Ser Tyr Glu Thr Ala Gin 100 105 110 WO 01/00805 Phe Ser Leu Asn 115 Gly Gly Met Ile 130 Ala Ala Gly Asn 145 Lys Ala Gly Val Gly Thr Gly Val 180 Ala Glu Ile Pro 195 Asp Phe Leu Trp 210 Ala Leu Ser Lys 225 Ala Met Asn Phe Val Met Gly Trp 260 Ala Gi y 135 Ile Ph e Thr Glu T yr 215 Arg Ser Lys T hr Cys 140 Thr His Gly Asp Ala 220 Gly Al a PCT11B00100926 Leu Se r Thr 160 Arg Glu Pro Arg Lys 240 Pro <210> 175 <211> 1293 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(1270) <223> RXN02062 (400> 175 ttgtctaaac atcgttttgg ggtccgaatg atagcccctt tcgctgcgca actgttttta gatggctaat ctttgaaatt atg Met gtc ValI cac His gtc cca Pro cga Arg cct Pro gaa ttaatgcccc catttcqgta atg aga qtc gga atg Met Arg Val Gly Met 1 ggc gct ggc gtg cac Gly Ala Gly Val His gct gaa gtt gat gtt Ala Glu Val Asp Val gtt ttc gtc cac ggc Val Phe Val His Gly att aag aca ctg tcc WO 01/00805 WO 0100805PCT/IBOO/00926 Val Asp Pro acc Th r tca Ser cac His cca Pro tct Ser gc t Ala 150 gac Asp cct Pro ggC Gi y cgc Arg gag Giu 230 atc Ile ga a Glu gag Glu cca Pro ggt Gly cac His ggc Gly tgg Trp gaa Glu 135 cgc Arg aac As n cgc Arg gtt Val1 caa Gin 215 tc Se r gca Ala ggC Gly att Ile ctg Leu tta Leu act Th r att Ile aag Lys 120 aaa Lys a tg Met g tg Val1 CCg Pro gac Asp 200 aaa L ys gtg Vali gc t Al a att Ile ctc Le u 280 ggc Gly Al a cgc Arg tgg Trp cct Pro 105 cg t Arg aat As n aaa Ly s cg t Arg act Th r 185 cca Pro ggc Gly ca g Gin cg c Arg ttc Phe 265 acc Thr atc Ile Leu Glu Ser Ala Asn Pro Ala Ile Lys Thr Leu Ser 60 a tg Met tat T yr cac His gag Glu g cc Ala ga t Asp gtt Val1 170 ttc Ph e cag Gin gtc Val ctt Leu acc Thr 250 tgg T rp gct Ala gtg Val1 aac As n cac His tct Ser tac Tyr g cg Al a tac Tyr 160 gac Asp tcc Ser ttt Phe gca Al a gcg Ala 240 gaa Giu Gly t gc Cys gcc Ala gtg Val ctt Leu ttg Leu ga c Asp gtc Val1 145 cct Pro act Thr gta Val gtc Val1 gca Ala 225 cca Pro ctc Le u aag Lys cca Pro tgc Cys ga t Asp gca Ala gag Glu gtg Val1 130 atc Ile cgc Arg gag Glu ctc Leu ggc Gly 210 gcg Al a gac Asp ca g Gin gac Asp tcc Ser 290 aac As n gtg Val1 cgt Arg 100 ga t Asp tcc Se r g tg Val1 gag Glu tgg Trp 180 tcc Ser atc Ile t tc Ph e ccc Pro a ag Lys 260 atc Ile tac Tyr gca Al a 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 WO 01/00805 295 300 gtc gca tcc gac gtt gga ggc atc cct Val Ala Ser Asp Val Gly Gly Ile Pro 310 315 acc ggc gcc ctc gtt cac tac gac gaa Thr Gly Ala Leu Val His Tyr Asp Glu 330 cgc gat atc gcc gaa gcg gtg aat aaa Arg Asp Ile Ala Glu Ala Val Asn Lys 345 350 gca gcc aaa ttt ggt ctc gca qgg cgc Ala Ala Lys Phe Gly Leu Ala Gly Arg 360 365 tcc tgg gca acg att gct cag cag acc Ser Trp Ala Thr Ile Ala Gin Gin Thr 375 380 atg taaaaccgaa agccggggaa cct Met 390 <210> 176 <211> 390 (212> PRT <213> Corynebacterium glutamicum <400> 176 Met Arq Val Gly Met Met Thr Arg Giu PCT/IBOO/00926 gag Glu aat Asn 335 a tg Met gaa Glu att Ile gt t Val1 320 gat Asp gtc Val1 cg t Arg ga t Asp 305 gtt Val1 gtc Val1 gct Ala gct Ala gtg ValI 385 gtc Val1 gaa Giu ga t Asp atc Ile 370 tac Tyr ga c Asp acc Thr cga Arg 355 aat As n aaa Lys ggc Gi y ttc Phe 340 gag Giu gat Asp tcc Ser 1075 1123 1171 1219 1267 1293

I

Gi y Ala Val Ile Val1 Leu Leu Asp Val1 Ala Glu Ph e Lys Asp Ala Glu ValI 130 Ile Thr Cys Asp Gly His Gly T rp Giu 135 Arg Val1 Arg Met As n Al a Gly Ala 110 Gly Ala Ala Tyr Giu G lu Pro Asn Giy His Gly Asp Ala WO 01/00805 PTIO/02 PCT/tBOO/00926 145 150 155 160 Pro Arg Ile Giu Pro Asp Asn Val Arg Val Val Leu Asn Gly Ile Asp 165 170 175 Thr Giu Leu Trp Gin Pro Arq Pro Thr Phe Asp Asp Ala Giu Asp Ser 180 185 190 Val Leu Arg Ser Leu Gly Vai Asp Pro Gin Arg Pro Ile Vai Ala Phe 195 200 205 Val Gly Arg Ile Thr Arg Gin Lys Gly Val Giu His Leu Ile Lys Ala 210 215 220 Ala Aia Leu Phe Asp Giu Ser Vai Gin Leu Val Leu Cys Ala Gly Ala 225 230 235 240 Pro Asp Thr Pro Giu Ile Ala Ala Arg Thr Thr Ala Leu Val Giu Giu 245 250 255 Leu Gin Ala Lys Arq Giu Gly Ile Phe Trp Val Gin Asp Met Leu Gly 260 265 270 Lys Asp Lys Ile Gin Giu Ile Leu Thr -Ala Ala Asp Thr Phe Val Cys 275 280 285 Pro Ser Ile Tyr Giu Pro Leu Gly Ile Val Asn Leu Giu Ala Met Ala 290 295 300 Cys Asn Thr Ala Val Val Ala Ser Asp Val Gly Gly Ile Pro Giu Val 305 310 315 320 Val Val Asp Gly Thr Thr Gly Ala Leu Val His Tyr Asp Giu Asn Asp 325 330 335 Val Giu Thr Phe Giu Arg Asp Ilie Ala Giu Ala Val Asn Lys Met Val 340 345 350 Ala Asp Arg Giu Thr Ala Ala Lys Phe Gly Leu Ala Gly Arg Giu Arq 355 360 365 Ala Ile Asn Asp Phe Ser Trp Ala Thr Ile Ala Gin Gin Thr Ile Asp 370 375 380 Val Tyr Lys Ser Leu Met 385 390 <210> 177 <211> 1266 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1243) <223> RXN02148 <400> 177 gcctcagact acggaatccc tgtgcaggtg gtatctgttg attcagcacc gttttatggt WO 01/00805 gcgCgtCgCt actaagaaat agttcgtcag gagaatcttt PCTIIBOO/00926 gtg tct gca tcc cga 115 Vai Ser Ala Ser Arg aaa Lys caa Gin gtg Val1 aag Lys ccc Pro gag Giu gca Ala cat His t tg Leu tac Tyr 150 ga a Giu cca Pro acc Thr gac Asp gcg gt t Val ttg Le u gcg Ala ga c Asp aca Thr aat As n gcg Al a 105 gag Glu atc Ile cg c Arg cct Pro aaa Ly s 185 att Ile atc Ile ctc gtg ValI agg Arg t cg Ser tat Tyr acg Thr gtg Val1 a ag Lys gtg Val1 Gly aga Arg t cc Ser 170 a gc Ser gcc Ala aag Lys att acc Thr gat Asp act Thr gag Gi u gca Ala 75 t gg Trp cag Gin ggg Gly act Thr ttC Phe 155 ggc Gly gca Al a tgt Cys gcg Al a gtg cca Pro caa Gin gaa Glu cct Pro gag Glu 80 gct Ala aag Lys cct Pro ctg Leu gga Gly 160 aga Arg cta Leu gtg Val1 att Ile tac cg g Arg ga t Asp g cg Ala cg t Arg atc Ile ccg Pro gtt Val gga Gly act Th r 145 t cg Ser ttc Ph e gga Gly c cg Pro g cg Ala 225 gag atc Ile cct Pro cat His t cg Ser att Ile ttg Leu att Ile tcg Se r 130 tat Tyr ca c His act Thr ttc Ph e agg Arg 210 gc g Al a t cg ggc Gly gag Giu gcc Ala gtg ValI cgt Arg g cg Al a gcc Ala 115 aga Arg atc Ile ccg Pro ccg Pro a cg Thr 195 aaa Ly s cgc Arg act gga Gi y tcg Ser tac Tyr atg Met gag Glu t tg Leu 100 t cc Ser caa Gin tcg Ser act Thr gc Ala 180 gac Asp ggc Gly cca Pro t tg atc Ile atc Ile gac Asp ctg Leu cga Arg atg Met act Thr t cg Ser cag Gin ttt Phe 165 a cg Thr acc Thr cag Gin ga t Asp C99 wo 01/00805 WO 0100805PCT/IBOO/00926 Ala Gin Leu Leu Ile Val Gly Ser Gly Arg 230 235 cgc ctt 9CC act gac gtg agc cag aac gtg Arg Leu Ala Thr Asp Val Ser Gin Asn Val 250 255 gaa tat caa gat atg atc aac acg ctt 9cc Glu Tyr Gin Asp Met Ile Asn Thr Leu Ala 265 270 atg cca gcg cgc acc cgc ggt ggc gga ctt Met Pro Ala Arg Thr Arg Gly Gly Gly Leu 280 285 att gtc tat ctc gag gca caa gcc tgc gga Ile Val Tyr Leu Glu Ala Gin Ala Cys Gly 295 300 acc tct ggc ggc gcg cca gag acg gtg act Thr Ser Gly Gly Ala Pro Glu Thr Val Thr 310 315 gtg gag ggg tcg gac gtc gat aag ctg tct Val Giu Gly Ser Asp Val Asp Lys Leu Ser 330 335 ctc gac gat ccg atc cgc cgc gcc gcg atg Leu Asp Asp Pro Ile Arg Arg Ala Ala Met 345 350 cat gtg gag gcc gaa tgg tcg tgg gaa atc His Val Giu Ala Giu Trp Ser Trp Giu Ile 360 365 aat att ttg cag agt gaa cca cga tgatggtt Asn Ile Leu Gin Ser Glu Pro Arg 375 380 <210> 178 <211> 381 <212> PRT <213> Corynebacterium giutamicum <400> 178 Val Ser Ala Ser Arg Lys Thr Leu Val Val 1 5 10 Arg Ile Gly Gly Ile Gin Ser Tyr Leu Arg 25 Asp Pro Glu Ser Ile Val Val Phe Ala Ser 40 Aia His Ala Tyr Asp Lys Thr Leu Asp Tyr 55 Arg Ser Val Met Leu Pro Thr Pro Thr Thr 70 Tyr 240 aaa Lys gcc Ala ga t Asp gtg ValI ccg Pro 320 gag Glu 9 gc Gi y atg Met Glu Ser Thr Leu ttc Phe 9 ca Ala gtt Val ccg Pro 305 gca Al a ctt Le u gct Ala 999 Gly ctt Leu gat Asp gaa Giu 290 gtg Val1 act Thr tta Leu gca Al a gag Gi u 999 cgc Gly Arg 260 att ttc Ile Phe 275 ggc ttg Gly Leu ata gcc Ile Ala ggc ctg Gly Leu att gag Ile Glu 340 ggt agg Gly Arg 355 cgg ttg Arg Leu 883 931 979 1027 1075 1123 1171 1219 1266 :gg acagctgttg ata Thr Asn Asp Phe Pro Asp Phe Ile Aia Thr Thr Gin Asn Ala Glu Glu Val Ile Arg Trp Ala His Ala Met Ala WO 01/00805 WO 0100805PCTIBOOIOO926 Ile Ilie Arg Glu Arg Glu Ile Asp Asn Val Trp Phe Gly Ala Ala Ala 90 Pro Leu Ala Leu Met Ala Gly Thr Ala Lys Gin Ala Gly Ala Ser Lys 100 105 110 Val Ile Ala Ser Thr His Gly His Giu Val Gly Trp Ser Met Leu Pro 115 120 125 Gly Ser Arg Gin Ser Leu Arg Lys Ile Gly Thr Giu Val Asp Val Leu 130 135 140 Thr Tyr Ilie Ser Gin Tyr Thr Leu Arg Arg Phe Lys Ser Ala Phe Gly 145 150 155 160 Ser His Pro Thr Phe Giu His Leu Pro Ser Gly Vai Asp Val Lys Arg 165 170 175 Phe Thr Pro Ala Thr Pro Giu Asp Lys Ser Ala Thr Arg Lys Lys Leu 180 185 190 Gly Phe Thr Asp Thr Thr Pro Val Ile Ala Cys Asn Ser Arg Leu Val 195 200 205 Pro Arq Lys Gly Gin Asp Ser Leu Ile Lys Ala Met Pro Gin Val Ile 210 215 220 Ala Ala Arg Pro Asp Ala Gin Leu Leu Ile Val Gly Ser Gly Arq Tyr 225 230 235 240 Giu Ser Thr Leu Arg Arg Leu Ala Thr Asp Val Ser Gin Asn Val Lys 245 250 255 Phe Leu Gly Arcj Leu Giu Tyr Gin Asp Met Ile Asn Thr Leu Ala Ala 260 265 270 Ala Asp Ile Phe Ala Met Pro Ala Arg Thr Arg Gly'Gly Gly Leu Asp 275 280 285 Val Giu Gly Leu Gly Ile Val Tyr Leu Glu Ala Gin Ala Gys Gly Val 290 295 300 Pro Val Ile Ala Gly Thr Ser Gly Gly Ala Pro Giu Thr Val Thr Pro 305 310 315 320 Ala Thr Gly Leu Val Val Giu Gly Ser Asp Val Asp Lys Leu Ser Glu 325 330 335 Leu Leu Ile Giu Leu Leu Asp Asp Pro Ile Arg Arg Ala Ala Met Gly 340 345 350 Ala Ala Gly Arg Ala His Val Giu Ala Giu Trp Ser Trp Giu Ile Met 355 360 365 Gly Giu Arg Leu Thr Asn Ile Leu Gin Ser Giu Pro Arg 370 375 380 <210> 179 (211> 1287 <212> DNA WO 01/00805 <213> Corynebacterium giutamicum <220> <221> CDS <222> (1264) <223> RXN02595 <400> 179 gtgggtaaag gggactccga ggaagtccac gtcgtcttct PCT/iBOOIOO926 ttcgcggcgc tgaggatggt ttcgcggatt tgtgcggggg agtgggtggg agagaaaacg gtg atc gtt gtg gcc Val Ile Val Val Ala atg Met atg Met ggC Gly ca g Gin Arg ttt Phe ggg Gly ggc Gi y ctc Leu ct g Leu 150 tac Tyr tcc Ser atc Ile agt Ser cat H-Iis tct Ser g tg Val qqC Gly ccq Pro 120 cat His a ag Lys act Thr att Ile ctt Leu gaa Giu gag Glu ttc Ph e tat Tyr acg Th r 105 ttt Ph e cat His gtt Val gcg Al a tta Leu tgg Trp gag Glu cg t Arg gt g Val1 t gg T rp qt t Val ttt Phe 125 gag Glu att Ile act Thr aaa Lys cg t Arg cgg Arg act Thr agg Arg gt g Val1 ga t Asp 110 ttc Ph e ca g Gin gag Gi u gtt Val1 g ca Ala ga t Asp gtg Va 1 gct Ala tat T yr gcc Ala gtg Val1 t cc Se r t gg Trp agc Ser tca Se r 175 cgg Arg gct Ala acc Thr qg t Gly ggg Gly t cc Ser 80 a tg Met gtg ValI ggt Gly ccg Pro cag Gin 160 gag Giu att Ile cct Pro gg t Gly ga t Asp cca Pro aag Lys gtg Val1 aat Asn ttg Leu gtg Val1 gct Al a 165 gag Glu aat As n 115 163 211 259 307 355 403 451 499 547 595 643 691 ctc att gcg ttq ggt gtg gat cag cag Leu Ile Ala Leu Gly Val Asp Gin Gin WO 01/00805 WO 0100805PCT/IBOOIOO926 ggc Gi y ca g Gin cat His 230 gic Val ci 9 Le u cac His cgc Arg tt Val1 310 gi C Val t ca Ser ggc Giy qga qtg Val cat His 215 9 C9 Ala gaa Glu gq i Gi y aag Lys aag Lys 295 ccg Pro ga c Asp gcc Ala 9CC Al a gcg gati Asp 200 gcg Ala a tg Met agc Se r gtg Val1 cac His 280 gaa Glu a cg Thr ggC Gly acc Thr agc Ser 360 cag ccc Pro gig Val1 gat Asp ggt Gi y ag C Ser 265 gcc Ala 99 c Gly atc Ile ga a Gi u aaa Ly s 345 gcg Al a tic ca c His 205 cgc Arg g cg Ala aag L ys gt i Val cgc Arg 285 qcq Ala a gc Ser ct Leu aic Ile gcc Ala 365 cti Leu acg Thr ctg Le u ctc Leu ga a Glu tc Phe 270 gcc Ala qic Val1 tca Ser gi c ValI ga c Asp 350 gaa Giu cii Leu aag Ly s ccg Pro ggc Gly 240 gic Val1 Gly ati Ile gag Glu ggc Gly 320 tcc Ser icc Ser i ac T yr ct Leu ga t Asp ait Ile ga t Asp cg c Arg 260 gag Giu cci Pro cac His icc Ser cii Leu 340 aag Lys acc Th r aag Lys ggc Gly gag Giu gta Val 245 acg Th r gati Asp i cg Se r ggc Gly gtc Val 325 ati Ile ctc Le u qcg Ala 739 787 835 883 931 979 1027 1075 11.23 1171 1219 1264 1287 Gly Ala Gin Phe Glu Glu Leu 375 380 tagtcccagc ggcaacgcca tcc <210> 180 <211> 388 <212> PRT <213> Corynebacterium glutamicum (400> 180 Val Ile Val Val Ala Met Ala Ser Ile Met Ala Cys Leu Lys Ala Ala 1 5 10 WO 01/00805 WO 0100805PCT/IBOO/00926 Arg Thr Giu His Arg Met Val Lys Val1 145 Ile Pro His Leu His 225 Val Asp Gin His Ala 305 Leu Lys As n Gin Ala Ala GI y Arg 100 Gin Val Arg Arg Gi u 180 Arg Asp Ile Asp Arg 260 Giu Pro His Ser Leu Pro Gly Asp Pro Lys Val2 Asn Leu Val1 Ala 165 Glu Asn G ly Glu Vai 245 Thr Asp Ser Gly ValI 325 Ile Met Gi y Gin Arg 70 Phe Gi y Gly Leu Leu 150 T yr Le u Gly Gin His 230 ValI L eu His Arg Val1 310 ValI Ser Lys Gly Gly Arg Ser Ile Ile Th r 135 Al a Lys Ile Val His 215 Ala Glu Gly Lys Lys 295 Pro Asp Al a Ile Ser His Ser Val1 Gi y Pro 120 His Lys Thr Ala Asp 200 Ala Met Ser Val His 280 Giu Thr Gi y Thr Leu 25 Glu Glu Ph e Tyr Thr 105 Phe His Val1 Ala Leu 185 Pro ValI Asp Gi y Ser 265 Ala Gly Ile Glu Lys Leu Arg Val Arg Pro 90 Ph e Phe Cys Gly Pro 170 Gly Val1 Th r Val Trp 250 Asp Leu Trp Gly Thr 330 Thr Leu Tyr ValI Asp 75 Lys S er Gly His Trp 155 Tyr Val1 Pro Leu ValI 235 T rp Arg Leu Gly Tyr 315 Gly Leu Cys Leu Phe Gly Al a Lys Lys Lys 140 Le u Val Asp Le u Ser 220 Al a Gin Val1 Glu Le u 300 Arg Leu Leu T rp Glu Arg Val Trp Val1 Ph e 125 Glu Ile Thr Gin His 205 Arg Al a Lys Val1 Arg 285 Al a Se r Leu Ile Arg Arg Thr Arg Val1 Asp 110 Phe Gin Glu Val1 Gin 190 Thr Leu Leu G iu Ph e 270 Ala Val1 Se r Val1 Asp Asp Val1 Ala Tyr Ala Val Se r Trp Ser 8er 175 Arg Pro Val1 Asp Leu 255 His Thr Thr Gly Asp 335 Ala Thr Gly Gly Ser Met Val1 Gi y Pro Gin 160 Glu Ile Lys Pro Gly 240 ValI Gi y Ile Glu Gly 320 Ser Se r WO 01/00805 WO 0100805PCTJIBOO/00926 340 345 350 Leu Arg Ser Lys Leu Gly Ala Ser Ala Lys Gin Arg Ala Glu Asn Tyr 355 360 365 Lys Trp Asp Thr Ala Gly Ala Gin Phe Giu Glu Len Leu Leu Gly Leu 370 375 380 Ala Ser Lys Lys 385 <210> 181 (211> 2334 ':212> DNA <213> Corynebacterium gintamicum <220> <221> CDS <222> (101)..(2311) (223> RXS00148 <400> 181 ctttgagggc agcgcgcatg cgcccgatgg ttatttqaac atgacaattg atgccgcggc gacgctggct gacctgctag atgctttggg agcttaaatc atg acg tcg atc cct 11 Met Thr Ser Ile Pro aat Asn cac His gtc ValI gg a Giy ccg Pro gca Ala ctt Leu tca Ser gtt Val cgc Arg ccg Pro cca Pro ttt Phe gcq Ala ga c Asp ga c Asp gta Val gtg Val1 act Thr tca Se r gt Gly 105 atc Ile qcc Al a ttc Ph e gat Asp atg Met acc Thr caa Gin ttg Len aag Lys cag Gin ttg Len acc Thr gcg Ala gg t Gi y gct Ala tgg Trp ga c Asp ggt Gi y cag Gin tcc Ser tcg Ser 110 gag Glu aac Asn cgc Arg caa Gin ccg Pro aat As n gt t Val1 aca Thr act Thr ga c Asp a ag Lys t gg Trp 80 gcg Ala qcq Ala 1 cg t Arg ccc Pro gag Giu cca Pro acg Th r ttt Ph e ttc Phe g ca Ala gaa Gin gcq Ala ttt Phe att Ile tat T yr ga t Asp tca Ser ga t Asp gcq Ala ggg Gly ta c Tyr aac As n acc Th r cac cgc qgt His Arg Gly 120 tat qac tcg gat aat Tyr Asp Ser Asp Asn cgc qtg gtc g~c Arg Vai Val Gly 130 gat gtg ggt Asp Val Gly WO 01/00805 WO 0100805PCTIiBOO/00926 ggc Gly att Ile ctg Leu ggt Gly ttt Ph e 200 atc Ile aac As n gcc Ala cgt Arg ctg Leu 280 aag Lys ga t Asp tct Ser a cc Thr cac His 360 gct gtg Val1 ga t Asp Ccg Pro ccg Pro 185 atg Met att Ile t cc Ser ga t Asp gca Ala 265 tcc Ser ctg Le u ccg Pro ggt Gly gcg Ala 345 acc Th r cg t gcg Ala ttg Le u att Ile 170 gag Giu gtg Val tcC Ser att Ile ttg Le u 250 gg t Gly ttc Phe cgt Arg aaa Lys t gg T rp 330 att Ile aat Asn atc att Ile t cc Se r 155 ctt Leu cag Gin c gc Arg aac Asn t cg Ser 235 gag Glu aaa Lys ttc P he gcg Aia aac Asn 315 t cg Ser gag Giu gca Ala gcc ga t Asp 140 agc Se r g cg Ala ctt Leu aac Asn atc Ile 220 att Ile ctg Leu gag Giu tgg Trp gga Gly 300 gc c Ala t tg Leu gcg Ala ctt Leu cga tcg Ser gtg Val1 ttc Phe gcg Al a acc Thr 205 ttt Ph e tct Se r gcc Ala gta Val1 gg t Gly 285 cga Arg aag Lys acc Th r atg Met ga t Asp 365 aac att Ile t cg Ser tat Tyr ggc Gly 190 tat T yr gag Glu ggc Gly tac Tyr ggc Gly 270 att Ile ctg Le u tcc Ser gcg Al a gct Ala 350 gag Giu acc ttg Le u gtg Val1 atc Ile 175 acg Thr att Ile tac Tyr tat Tyr act Thr 255 ctt Leu tct Ser ctg Le u cag Gin cag Gin 335 g ca Ala gcg Ala ca g gat Asp t cg Ser 160 gtg Val atc Ile tat Tyr a cc Thr cac His 240 ct g Le u ga c Asp a tg Met t gg T rp t cg Se r 320 gat Asp acc Thr ttg Leu Ctg a tg Met 145 atg Met gcg Ala cag Gin ccg Pro t cc Se r 225 atc Ile gcg Ala gtg Val1 tac Tyr ag c Se r 305 ctg Leu gtg Val1 ca g Gin gcg Ala ttg cgt Arg a cc Thr gct Ala aat Asn ccg Pro 210 ttg Leu cag Gin ga t Asp ga t Asp acc Thr 290 gag Giu cgc Arg tac Tyr ggc Gi y ctg Leu 370 c tg cag Gin a tg Met gag Giu gac Asp 195 aa g Lys aag Lys gaa Glu gg t Gly aag Lys 275 ttc Phe t tg Leu a cg Thr aac As n ca c His 355 ccc Pro cag ctg Leu aat As n ga a Glu 180 atc Ile ccg Pro atg Met g cg Ala att Ile 260 ttc Phe atq Met gtq Val1 cac His aac As n 340 acc Thr a cc Thr cag ttt Phe ggc Gly 165 caa Gin t tg Leu t cg Se r cca Pro gga Gly 245 gaa Glu g cg Al a gag Giu g ca Ala t cg Ser 325 gtc Val cag Gin ga t Asp g aa 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 wo 01/00805 WO 0100805PCTIIBOO/00926 Phe Ser Ala tct Ser 390 gag Glu gag Glu cct Pro gati Asp gaa Glu 470 gca Al a g cg Ala gag Glu gt c Val1 tig Leu 550 ggc Gly ga a Glu cg c Arg gga Gly 375 ggc Gly tgg T rp gig Val a ag Lys icc Ser 455 gat Asp ga a Gl u gaa Glu cat His gat Asp 535 gaa Glu gig Val1 cgc Arg cca Pro c ag Gln a cg Th r tig Leu gag Glu cig Leu 440 ggc Gly gag G li cag Gln gtc Val aaa Lys 520 gct Ala gt Val tac Tyr gcg Ala cg i Arg 600 aag Lys Arg gtg Val acc Thr gaa Glu 425 cg c Arg cgc Arg gaa Glu tig Leu aag Lys 505 gag Glu gcg Al a gtc ValI aag Lys aic Ile 585 atc Ile gi i Val Ile cg i Arg aat Asn 410 gcc Ala att Ile ca g Gln att Ile gct Al a 490 gct Ala cca Pro cg c Arg ttt Ph e ga t Asp 570 gcc Ala iii Phe gic ValI Ala cc a Pro 395 gag Glu ggc Gly gag Glu gcg Ala gaa Glu 475 aaa Lys gcg Ala g gg Gly gca Ala ggc G ly 555 gag Glu ctg Leu ati Ile g cg Ala Arg 380 gtt Val1 ctg Leu gga Gly gaa Glu ctg Le u 460 gtc Val cic Leu ctg Le u gati Asp aaa Ly s 540 cgc Arg gt t ValI gc t Al a gcc Ala tct Se r Asn gati Asp g ct Ala a tg Met tca Ser 445 atc Ile ctc Leu g cg Al a gati Asp t tg Leu 525 gct Ala cac His gga Gly gac Asp aa g Lys 605 gcc Ala Gln i gg Trp cgc Arg 415 cag Gln gca Ala gtg Val git Val cig Le u 495 ttg Leu ca g Gln ati Ile gca Ala gaa Glu 575 ttt Ph e ggc Gb y gci Ala Leu gcg Ala 400 gcg Ala gcc Ala cgc Arg aat As n gac Asp 480' aaa Lys aca Thr aac Asn gga Gly ga a Glu 560 ggc Gly gag Glu ca g Gln ga c Asp Leu 385 ggc Gly cgc Arg acc Thr acc Th r cg c Arg 465 aa c As n gca Al a gct Ala cig Leu gag Glu 545 atc Ile aca Thr gct Ala ga t Asp ctg Leu Le u tcc Ser aag Lys gcg Ala cag Gln 450 tac Tyr acc Thr gag Glu gci Ala ctc Leu 530 aic Ile agg Arg gtg Val1 gag Glui gga Gly 610 ggc Gly Gln ta c Tyr aic Ile 420 gga Gly cgc Arg gcg Ala gitt ValI aac As n 500 cg c Arg cti Leu ga t Asp ctg Leu aac Asn 580 ggc Gly gac Asp ga c Asp Glu gig ValI 405 gati Asp att Ile att Ile gaa Glu cg c Arg 485 ga t Asp aac As n gcc Ala gc i Ala ici Se r 565 gic Val cgc Arg cg i Arg gig Val 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 WO 01/00805 615 620 gat gtt gga ccg ctg ttt caa act cca Asp Val Gly Pro Leu Phe Gin Thr Pro 630 635 gtg gac gcc gat gtt cac gtg gtg ggt Val Asp Ala Asp Val His Val Val Gly 650 cac ctc acc ttg ctg ccc gag ctg aag His Leu Thr Leu Leu Pro Giu Leu Lys 665 670 cgc gat gac att ctg gtc acc gtg ggc Arg Asp Asp Ile Leu Val Thr Val Gly 680 685 ttc cag gat ctc tac gat atg ggt gcc Phe Gin Asp Leu Tyr Asp Met Gly Ala 695 700 acc gtc atc gcg gag tcg gcg atc gat Thr Val Ile Ala Giu Ser Ala Ile Asp 710 715 cac ctg ggc ttt gac ctg gat gtg gat His Leu Gly Phe Asp Leu Asp Val Asp 730 ttcctagaag aca <210> 182 <211> 737 <212> PRT <213> Corynebacterium giutamicum <400> 182 Met Thr Ser Ile Pro Asn Phe Ser Asp 1 5 Arg Ala Ser Glu Ser His Asn Vai Asp 25 Pro Glu Gly Ile Asp Val Lys Arg Vai Giu Ala Gin Ala Ala Giy His Pro Vai Pro Phe Met Arg Gly Pro Tyr Pro Thr Thr Ile Arg Gin Tyr Ala Giy Phe Ser Phe Tyr Arg Arg Asn Leu Ala Ala Gly 100 105 Phe Asp Leu Ala Thr His Arg Gly Tyr PCTIBOO/00926 gcc cgc gcc Ala Arg Ala ctg gca gca Leu Ala Ala 660 gca gct ctt Ala Ala Leu 675 ccg ccg ggc Pro Pro Gly 690 tac cct tca Tyr Pro Ser cga ctc gcc Arg Leu Ala tgatcacggt gcc Ala 645 ggc Gly ggc Gly ga t Asp gga Gly gca Ala 725 2035 2083 2131 2179 2227 2275 2321 2334 Ala Trp Asp Gly Gin Se r Se r 110 Giu Giu Asn Arg Gin Pro As n Val1 Arg WO 01/00805 PCT/IB00/00926 Val Met 145 Met Ala Gin Pro Ser 225 Ile Ala Val Tyr Ser 305 Leu Val Gin Ala Leu 385 Gly Arg Thr Gly 130 Arg Thr Ala Asn Pro 210 Leu Gin Asp Asp Thr 290 Glu Arg Tyr Gly Leu 370 Leu Ser Lys Ala Asp Gin Met Glu Asp 195 Lys Lys Glu Gly Lys 275 Phe Leu Thr Asn His 355 Pro Gin Tyr His Gin 435 Val Leu Asn Glu 180 Ile Pro Met Ala Ile 260 Phe Met Val His Asn 340 Thr Thr Gin Tyr Ile 420 Gly Gly Phe Gly 165 Gin Leu Ser Pro Gly 245 Glu Ala Glu Ala Ser 325 Val Gin Asp Glu Val 405 Asp Ile Met Asp 150 Ala Gly Lys Met Arg 230 Ala Tyr Pro Ile Lys 310 Gin Ala Ser Phe Ser 390 Glu Glu Pro Ala Gly 135 Gly Ile Val Leu Val Gly Glu Phe 200 Arg Ile 215 Phe Asn Thr Ala Ile Arg Arg Leu 280 Ala Lys 295 Phe Asp Thr Ser Arg Thr Leu His 360 Ser Ala 375 Gly Thr Trp Leu Val Glu Lys Leu 440 Val Asp Pro Pro 185 Met Ile Ser Asp Ala 265 Ser Leu Pro Gly Ala 345 Thr Arg Val Thr Glu 425 Arg Ala Leu Ile 170 Glu Val Ser Ile Leu 250 Gly Phe Arg Lys Trp 330 Ile Asn Ile Arg Asn 410 Ala Ile Ile Ser 155 Leu Gin Arg Asn Ser 235 Glu Lys Phe Ala Asn 315 Ser Glu Ala Ala Pro 395 Glu Gly Glu Asp 140 Ser Ala Leu Asn Ile 220 Ile Leu Glu Trp Gly 300 Ala Leu Ala Leu Arg 380 Val Leu Gly Glu Ser Val Phe Ala Thr 205 Phe Ser Ala Val Gly 285 Arg Lys Thr Met Asp 365 Asn Asp Ala Met Ser 445 Ile Ser Tyr Gly 190 Tyr Glu Gly Tyr Gly 270 Ile Leu Ser Ala Ala 350 Glu Thr Pro Asn Ala 430 Ala Leu Val Ile 175 Thr Ile Tyr Tyr Thr 255 Leu Ser Leu Gin Gin 335 Ala Ala Gin Trp Arg 415 Gin Ala Asp Ser 160 Val Ile Tyr Thr His 240 Leu Asp Met Trp Ser 320 Asp Thr Leu Leu Ala 400 Ala Ala Arg WO 01/00805 WO 0100805PCT/IB00100926 Thr Arg 465 As n Ala Ala Leu Glu 545 Ile Thr Al a Asp Leu 625 Al a Ser Leu Ile Ile 705 Ile Glu Arg 485 Asp As n Al a Ala Ser 565 Val1 Arg Arg Val Ala 645 Gly Gly Asp GI y Asp Glu 470 Ala Ala Glu Val Leu 550 Gly Glu Arg Gly Asp 630 Val His Arg Ph e Thr 710 Gln Ile Ala 490 Ala Pro Arg Phe Asp 570 Ala Phe Val1 Leo Val1 650 Leu Leu Ty r Glu Asp 730 Ile Leo Ala Asp Leo 525 Ala His Gly Asp Lys 605 Ala Thr Val Leu Val1 685 Gly Ile Gi y Lys Gln Ala 510 Asp Thr Glu Lys Ala 590 Met Tyr Pro Gly Lys 670 Gly Ala Asp Val Val1 Leo 495 Leo Gln Ile Ala Glu 575 Phe Gl y Ala Ala Met 655 Ly s Gly Ala Leo Thr Arg Leo Ala Ala His Leo Gly Phe 725 Glu Leo Asp Val Asp Val Asn <210> 183 <211> 1971 WO 01/00805 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (1948) <223> RXS00149 <400> 183 ttttcgtagg taaacacagg tgaaggcttt acaagcttgt PCTIBOOIOO926 gaactcccta cacaaaagca atccaatagc tatccataag caagagaaag taagtctacg ttg act gat ctc aca Leu Thr Asp Leu Thr aag Lys aa q Lys gac Asp 99 t Gly aaa Ly s gt t ValI gac Asp ggc Gi y gat Asp ctg Leu 150 a cg Thr tct Ser gcg Ala gtg Val1 gCC Ala ga t Asp act Thr ggt Gly C Cg Pro acc Thr 120 aaa L ys cat His gcg Ala cct Pro gag Giu gt t ValI gtg Val1 ccg Pro cc t Pro 75 gtt Val atc Ile ggt Gly ctc Leu gga Gly 155 gcc Ala g cg Ala qaa ctt Glu Leu ttc gcg Phe Ala tgg aag Trp Lys 45 ctg tac Leu Tyr ggt gag Gly Glu ggt tgg Gly Trp aac gct Asn Ala ttt gag Phe Glu 125 gaa ggc Giu Gly 140 tcc acg Ser Thr gga acg Giy Thr cag gtt Gin Val gag Glu aca Th r ctc Leu ag a Arg ccc Pro gt t Val gt g Val1 tct Se r tat Tyr gag Giu ttt Phe 175 gg t Gly gaa Giu aaa Lys act Th r gag Glu agg Arg act Thr gct Ala ttc Ph e 130 atg Met g cg Ala ttg Leu act Thr gac Asp .a ca Th r tcc Ser gga Giy t tc Phe ctg Leu 115 acg Thr gct Ala gcg Ala acc Thr tgg T rp atc Ile ccg Pro cag Gin acc Thr gga Gly 100 aat As n gca Ala ccg Pro ttg Leu ttg Le u 180 tac Tyr ggC Gly ga t Asp agg Arg act Th r cat His tct Ser gct Ala ttg Le u tat Tyr 165 ggt Gly 115 163 211 259 307 355 403 451 499 547 595 643 691 tcg cac agt gac acc att Ser His Ser Asp Thr Ile WO 01/00805 WO 0100805PCTLBOO/00926 ga a Glu cgc Arg ga t Asp 230 cgt Arg gtg Val1 aag Lys gg t Gly gcg Ala 310 gtt Val1 cg t Arg aat As n gag Glu gtg Val1 390 agt Se r ga a Glu gct Ala 215 gc t Ala cgc Arg gcg Ala ctg Le u ttt Ph e 295 atg Met gca Al a act Th r ttc Ph e t ca Ser 375 gag Gl u ggc Gl y gca Ala 200 atc Ile caa Gln ttg Leu ttc Phe cgt Arg 280 cca Pro ttt Phe gct Ala ttt Phe gct Ala 360 cat His agc Ser atc Ile gtt Val1 ttg Leu ga a Glu gtc Val1 cg t Arg 265 gtg Val1 gag Glu agc Ser ttc Ph e ga t Asp 345 cac His ct t Leu ttc Phe ga a Glu cag Gln gtg Val att Ile gat Asp 250 ttt.

Phe gct Ala ctg Leu cag Gln gc t Al a 330 ga t Asp cgc Arg ggt Gly acc Thr gct Ala 410 ttg Le u ga t Asp ggt Gly 235 gca Ala gcg Ala cga Arg gc c Al a cg t Arg 315 gca Ala g cg Ala atc Ile cac His gat Asp 395 gag Glu gca Ala gg t Gly 220 ct a Le u 9gc Gly gt c Val1 cg t Arg gta Val1 300 ga t Asp ggc Gly atc Ile gcg Ala gtg Val 380 ga t Asp ggc Gly gtg aat gct Val Asn Ala 205 tcc agt ttt Ser Ser Phe agt atc gcc Ser Ile Ala ctt tcc acg Leu Ser Thr 255 acc gat gag Thr Asp Glu 270 ctg tgg gcc Leu Trp Ala 285 gca cca cag Ala Pro Gln ccg tgg gtg Pro Trp Val gtc ggt gga Val Gly Gly 335 cca gat gga Pro Asp Gly 350 cgc aat act Arg Asn Thr 365 gtt gat cct Val Asp Pro cta gcg gag Leu Ala Glu gga tac agt Gly Tyr Ser 415 cag acg tgg Gln Thr Trp 430 t cc Ser t cc Ser gcc Ala 240 gaa Glu cag Gln a gg Arg cat His aat As n 320 gca Ala gtt Val1 aat Asn gc t Al a aag Lys 400 g ca Ala gag Glu aag Lys aac Asn 225 gg t Gl y gc t Ala ttc Ph e gtg Val1 gcg Ala 305 atg Met acc Thr cct Pro ttg Le u ggt Gly 385 g cg Ala gct Ala ca g Gln cg t Arg 210 cag Gln gtg Val1 g ca Al a gcg Ala tg t Cys 290 gtg Val1 ctg Leu ga t Asp gga Gly ttg Le u 370 gga Gly t gg T rp tgt Cys act Thr gcg Ala ggc Gly ga t Asp ctt Leu cag Gln 275 gag Glu act Thr cgc Arg gt g Val g tg Val1 355 ttg Le u tca Ser gct Ala gca Ala cgc Arg 435 aat gtg Asn Val gcg tcg Ala Ser tat gtc Tyr Val 245 aag cag Lys Gln 260 att tct Ile Ser gtg ctt Val Leu gca cga Ala Arg agt act Ser Thr 325 gag gtt Glu Val 340 tcg agg Ser Arg cta gaa Leu Glu tat ttc Tyr Phe gtg ttc Val Phe 405 tcc ggc Ser Gly 420 gct gat Ala Asp 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 acg gtg act gcc atg ctt gat Thr Val Thr Ala Met Leu Asp 425 WO 01/00805 WO 0100805PCTf[BOO/00926 gtg Val1 ttg Le u gtg ValI 470 gct Ala ctg Le u ct 9 Leu ccc Pro gtg Val 550 gt C Val1 g cg Ala aac Asn ttg Le u g cg Ala 9c9 Ala 455 cgt Arg ttc P he gga Gly t tg Leu ggc Gly 535 gtg ValI gaa Glu ccg Pro atg Met gga Gly 615 t cg Ser 440 gag Glu cga Arg t tg Leu ccg Pro gct Ala 520 act Thr tgt Cys aag Lys aag Ly s aca Th r 600 gct Ala aga aag aag Arg Lys Lys tct ccg ctg Ser Pro Leu tgg gca gcg Trp Ala Ala 475 gaa aag aac Giu Lys Asn 490 ttg tcc aag Leu Ser Lys 505 tcc ggt ggc Ser Gly Gly gac gct ttt Asp Ala Phe gga acg gac Gly Thr Asp 555 ctc cgc gaa Leu Arg Glu 570 agc ttt gag Ser Phe Glu 585 att gat gcc Ile Asp Ala aag ctc act Lys Leu Thr 445 cca Pro 460 gat Asp ggC Gly cac His att Ile gca Ala 540 ca a Gin gcg Ala ggc Gly gcg gct gat Ala Asp ttt gaa Phe Glu gcg agg Ala Arg aat att Asn Ile 510 gaa gca Glu Ala 525 gaa gct Giu Ala gag tat Glu Tyr ggc gtt Gly Val agc gcg Ser Ala 590 gcg acg gga Gly cgt Arg g cg Ala cca Pro 495 cg C Arg atc Ile gca Ala qcc Ala gag Glu 575 cat His ctg att aat gag ttc ccg aac Ile Asn Glu Phe Pro Asn 450 cgg Ar~g ctg Leu 480 cag Gin act Thr aac As n cag Gin ga a Gl u 560 cg C Arg gcg Al a gct Ala gta Val1 465 cgc Arg atc Ile gt Gly ccg Pro gcc Ala 545 a cg Thr atc Ile ccc Pro gac Asp gaa Glu aat As n a cg Th r ttt Phe gg t Gl y 530 gca Ala ggg Gi y ctg Le u gat Asp ctg Le u 610 cct Pro cg t Arg a tg Met act Thr 515 caa Gln ggc Gl y gag Glu ctt Leu gg t Gly 595 ct a Leu gca Ala tcg Ser att Ile 500 tcc Se r ctt Leu att Ile gga Gly gct Ala 580 tat Tyr ga t Asp gg t Gly ga t Asp 485 cct Pro aac Asn gtt Val gta Val1 gcc Al a 565 gg C Gly ttg Le u gct Al a 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1971 Ala Ala Thr Leu taaatcatga cgtcgatccc taa e210> 184 <211> 616 <212> PRT <213> Corynebacterium glutamicum (400> 184 Leu Thr Asp Leu Thr Lys Thr Ala Val Pro Glu Glu Leu Ser Glu Asn 1 5 10 Leu Glu Thr Trp Tyr Lys Ala Vai Ala Gly Val Phe Ala Arg Thr Gln 25 wo 01/00805 WO 0100805PCTJIBOO/00926 Lys Lys Val Thr Asp Glu Thr Arg Glu Thr Asn Ala Glu Phe 130 Asn Met 145 Ala Ala Ala Leu His Ser Lys Arg 210 Asn Gln 225 Gly Val Ala Ala Phe Ala Vai Cys 290 Ala Val 305 Met Leu Asp Th r Ser Gly Phe Leu 115 Thr Ala Al a Thr Asp 195 Ala Gly Asp Leu Gin 275 Glu Th r Arg Ile Pro Gln Thr Gi y 100 As n Al a Pro Le u Le u 180 Th r As n Al a Tyr Ly s 260 Ile ValI Ala Ser Giy Asp Arg Thr His Se r Ala Leu Tyr 165 Gly Ile Val1 Se r Val1 245 Gin Se r Leu Arg Thr 325 Asp Gly Lys 70 Val Asp Gly Asp Le u 150 Th r Ser Giu Arg Asp 230 Arg Val Lys Gly Ala 310 Val1 Ile Val1 55 Ph e Asp Se r Thr Leu .135 Ile Leu Arg Giu Ala 215 Ala Arg Ala Le u Phe 295 Met Ala Ala 40 Asp Th r Gly Pro Th r 120 Ly s His Al a Pro Ala 200 Ile Gin Leu Phe Arg 280 Pro Ph e Ala Val1 Ile Glu Glu Lys 105 Thr Val1 Ala Glu Leu 185 Val1 Leu Glu ValI Arg 265 Val1 Giu Ser Phe Asp 345 Asp As n Val1 Arg 90 Asn Leu Ala Gly Glu 170 Th r Gin Val1 Ile Asp 250 Ph e Al a Le u Gin Ala 330 Val Pro Pro 75 Val Ile Gi y Leu Gly 155 Ala Ala Leu Asp Gly 235 Ala Al a Arg Ala Arg 315 Ala Trp Leu Gly Gly As n Phe Giu 140 Se r Gly Gin Ala Gi y 220 Leu Giy Val Arg Val 300 Asp Gly Lys Tyr Giu Trp Ala Glu 125 Gly Thr Th r Val1 Val1 205 Se r Se r Leu Thr Le u 285 Ala Pro Val Lys Thr Phe Gly Ala 110 Phe Val Ser Phe Asp 190 As n Ser Ile Se r Asp 270 Trp Pro Trp Gly Le u Arg Pro Val1 Val1 Ser Tyr Glu Phe 175 Gly Ala Phe Ala Th r 255 Giu Ala Gin Val1 Gly 335 Ile Al a Phe Th r Leu Glu Leu Val1 160 Al a Ser Ser Ser Al a 240 Giu Gin Arg His As n 320 Ala Thr Asp Val Glu Val Arg Thr Phe 340 Asp Ala Ile Pro Asp Gly Val 350 WO 01/00805 PCT/IB00/00926 Pro Gly Val Ser Arg Asn Phe Ala His Arg Ile Ala Arg Asn Thr Asn 355 360 365 Leu Leu Leu Leu Glu Glu Ser His Leu Gly His Val Val Asp Pro Ala 370 375 380 Gly Gly Ser Tyr Phe Val Glu Ser Phe Thr Asp Asp Leu Ala Glu Lys 385 390 395 400 Ala Trp Ala Val Phe Ser Gly Ile Glu Ala Glu Gly Gly Tyr Ser Ala 405 410 415 Ala Cys Ala Ser Gly Thr Val Thr Ala Met Leu Asp Gin Thr Trp Glu 420 425 430 Gin Thr Arg Ala Asp Val Ala Ser Arg Lys Lys Lys Leu Thr Gly Ile 435 440 445 Asn Glu Phe Pro Asn Leu Ala Glu Ser Pro Leu Pro Ala Asp Arg Arg 450 455 460 Val Glu Pro Ala Gly Val Arg Arg Trp Ala Ala Asp Phe Glu Ala Leu 465 470 475 480 Arg Asn Arg Ser Asp Ala Phe Leu Glu Lys Asn Gly Ala Arg Pro Gin 485 490 495 Ile Thr Met Ile Pro Leu Gly Pro Leu Ser Lys His Asn Ile Arg Thr 500 505 510 Gly Phe Thr Ser Asn Leu Leu Ala Ser Gly Gly Ile Glu Ala Ile Asn 515 520 525 Pro Gly Gin Leu Val Pro Gly Thr Asp Ala Phe Ala Glu Ala Ala Gin 530 535 540 Ala Ala Gly Ile Val Val Val Cys Gly Thr Asp Gin Glu Tyr Ala Glu 545 550 555 560 Thr Gly Glu Gly Ala Val Glu Lys Leu Arg Glu Ala Gly Val Glu Arg 565 570 575 Ile Leu Leu Ala Gly Ala Pro Lys Ser Phe Glu Gly Ser Ala His Ala 580 585 590 Pro Asp Gly Tyr Leu Asn Met Thr Ile Asp Ala Ala Ala Thr Leu Ala 595 600 605 Asp Leu Leu Asp Ala Leu Gly Ala 610 615 <210> 185 <211> 1179 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1156) <223> RXS02106 WO 01/00805 <400> 185 ggtcgccgct tttcgacgcc cgcctgcggc gggatgactg PCTIBOOIOO926 tgatggaggg gcgcgtcgac aagcaaaatc tctttagcaa attcggttac tgtggggcgc atg aat aac cat ttt Met Asn Asn His Phe 115 gag Glu ga t Asp ga a Glu t cg Ser ttg Le u att Ile ga t Asp aac Asn cg t Arg atc Ile 150 aat Asn a tg Met gaa Giu ctc Leu ctg Leu ccc Pro gtg Val1 gcg Ala gct Ala tat Tyr gtt Val ggc Gi y 135 ggt Gly gaa Giu ttt Phe t ct Ser aaa Lys gat Asp ga t Asp gg t Gly ga a Glu tta Leu gaa Giu gcg Ala 120 c cg Pro agt Ser cat His atg Met ctc Le u gta ValI tcc Ser gag Glu ga t Asp tat Tyr gct Ala tgg Trp 105 ttg Le u acg Th r ttc Ph e qgt Gly gag Giu 185 gt t Val cc t Pro att Ile gca Ala aac Asn gat Asp gtg Val1 gaa Giu ga t Asp atg Met cag Gin gtg Val1 170 ggc Gly gc t Ala qgt gga Gly Gly gct gac Ala Asp ttc ttc Phe Phe act gat Thr Asp 60 gac qtt Asp Val 75 cgt cgg Arg Arg atc ctg Ile Leu gag ctc Giu Leu cgc att Arg Ile 140 tca tat Ser Tyr 155 acc qtg Thr Val ggc aac Gly Asn ggt ttg Giy Leu aag Lys gtg Val1 gcc Ala 45 cg t Arg gag Glu qca Ala cac His ctt Leu 125 t gg T rp gt c Val1 gta Val1 tgc C ys a gc Ser 205 ctt Leu a ag Lys ctt Le u ttg Le u cta Le u gtc Val1 cca Pro 110 t tg Le u ga t Asp aa t As n cg a Arg atc Ile 190 tat Tyr gt c ValI att Ile Gi y cag Gin cac His acc Thr ggg Gly gac Asp t gg Trp ga a Glu cgc Arg 175 acc Th r gag Giu aca gtc Val1 t cc Ser cgg Arg gca Ala 9gc Gly qgc Gly gtg Val1 caa Gin ga t Asp atc Ile 160 a tg Met tat Tyr cag Gin cac 1 gtt Vali Gly g cg Ala aag Lys ttt Ph e g cg Ala ctt Le u gtt Vali ga t Asp 145 aac As n tct Se r tcc Ser tcc Ser ga t ga t Asp gat Asp ct g Leu ttg Leu a gc Ser caa Gin cct Pro gcc Ala 130 cgc Arq caa Gin gg t Gly ctg Leu tat Tyr 210 gtt 200 gat cgt tgg gtg att gct gog ctq aag WO 01/00805 WO 0100805PCTIBOOIOO926 Asp Arg 215 tac gtg Tyr Val 230 gct gca Ala Ala tcc tat Ser Tyr aag gtg Lys Val gat cct Asp Pro 295 acc cta Thr Leu 310 ctc agc Leu Ser Trp Val Ile Ala Ala Leu Lys Thr His cct Pro ca g Gin gac Asp aag Lys 280 ctg Leu aag L ys ga t Asp atc Ile aaa Lys atc Ilie 265 att Ile cy c Arg t cc Ser ga a Glu aat Asn cy t Arg 250 gat Asp tcc Ser cgc Arg aca Thr ga t Asp 330 gac Asp 235 cgc Arg g cg Aia gac Asp caa Gin ttc Phe 315 ttc Phe 220 atc Ile agt Ser gac Asp aag Ly s aca Th r 300 ag t Ser gcg Ala t cC Ser gca Ala a tg Met 270 ctt Leu g ca Ala agg Arg ggc Gly a cc Thr gtc Val1 255 a cc Th r cgc Arg tca Ser tac Tyr cac His 335 ggc Gly 240 ctc Le u cag Gin agc Ser cy t Arg gyc Gi y 320 gac Asp Asp 225 gga Gly cac His g tg Val1 gca Ala gag Glu 305 gcg Ala cta Val Asp Ala Trp aaa Ly s cac His ttq Leu aag Lys 290 ca a Gin caa Gln gt a atc Ile g tg Val cyc Arg 275 aag Lys atc I le gaa Giu aaa ggt Gly acc Thr 260 att Ile cg c Arg atc Ile gta Val1 a cc 835 883 931 979 1027 1075 1123 Leu Val Lys Thr Lys 340 tac qcc acc gag gag tgq act aag Tyr Ala Thr Glu Glu Trp Thr Lys 345 aag <210> 186 <211> 352 <212> PRT <213> Corynebacterium giutamicum <400> 186 Met Asn Asn His Phe Glu Leu Lys 1 5 Val Asp Val Thr Thr Asp Leu Asp Gly Asp Phe Phe Leu Giu Pro Asp Ala Leu Gln Gly Ala Ser Val Gly Lys Leu Asp Ala Ala Leu Ala Giu 70 Phe Ser Thr Ala Asp Ilie Ala Leu :ga qtt caa krg Val Gln 350 tagtttctat ggatctgcac 1176 1179 Val1 Ser Glu A~sp T yr Alia Pro 10 Ile Ala Asn Asp Val1 90 Gly Ala Phe Th r Asp 75 Arg ValI Ile Gly Gin His Thr Val1 Se r Arg Ala Gly Gly WO 01/00805 WO 0100805PCTIiBOO/00926 Ala Gin Asp Phe Thr Asp Tyr Giu Giu Ile Leu His Pro Giy Val 110 Leu Val Asp 145 As n Ser Ser Ser Asp 225 Gly His Vali Aila Giu 305 Aia Leu 100 Pro Gly Thr Gly Gly 180 Aia Tyr Al a Gly Thr 260 Ile Arg Ile Val1 Th r 340 Leu Gin Val1 Val 165 Ala Pro Leu Trp Gi y 245 Met Giy Val1 Asp Giu 325 Lys As n Arq Ile 150 As n Met Giu Asp Tyr 230 Al a Ser Lys Asp Th r 310 Le u Tyr ValI Gi y 135 Giy Glu Ph e Ser Arg 215 Val Ala Tyr Val1 Pro 295 Le u S er Ala Ala 120 Pro Ser His Met Leu 200 T rp Pro Gin Asp Lys 280 Leu Lys Asp Th r Leu 125 Trp Val1 Val Cys S er 205 Leu Th r Gly Met Gi y 285 Gi y Ala Al a Lys Leu Asp As n Arg Ile 190 Tyr Lys Ser Ala Met 270 Leu Ala Arg Gly Arg 350 Asp Trp Giu Arg 175 Thr Glu Thr Th r Val 255 Thr Arg Se r Tyr His 335 Val1 <210> 187 <211> 876 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (853) WO 01/00805 <223> RXS01746 <400> 187 aaaccgccac tcccctttca ctggggagtg gcggttttgt PCTJIBOO/00926 cgtttcatgc atqcagtgtg tgacttatca accttgttag ggctagggtg gatatctatc atg act gca cca aga ga t Asp att Ile tat Tyr ca g Gin acc Th r ga t Asp tat Tyr aga Arq gc c Aila cat His 150 act Thr t tg Leu ccc Pro cgg Arg ctt Leu t tg L eu ga t Asp cgc Arg aaa Lys ga a Giu cgc Arg gq Val1 q ca Aia 170 gag Giu gca Ala ttg Leu gct Ala gag Glu ctt Leu 75 atc Ile tta Leu gcg Al a att Ile qa c Asp 155 atg Met cac His gat Asp ggt Giy acc Th r cac His ccc Pro a cg Thr gcc Ala ctc Leu ga t Asp 140 a gc Ser cac His atc Ile tct Ser atc Ile agg Arg t cg Ser aa c Asn cat His ccg Pro 110 caa Gin cgt Arg qtt Val1 gtg Val ccg Pro 190 cg c Arg tat T yr aat As n tat Tyr ct g Leu 80 cc t Pro ga t Asp gtc Val1 gqt Gly qcc Al a 160 atc Ile Gly Met 1 g cg Ala caa Gin ga t Asp acc Thr ccg Pro ggc Gly gtq Val g9C Gly gtg Val1 145 atc Ile aac As n att Ile Th r tct Ser gag Glu ga a Glu gca Ala g tg Val1 caa Gin gtt Val1 ga t Asp 130 tgg Trp ggc Gly tgc Cys gct Ala Al a gca Ala gcc Ala atc Ile ggt Gly atc Ile ttg Leu ga t Asp 115 atq Met gtg Val1 att Ile aac As n ga t Asp 195 Pro Arg gag ccc Giu Pro tgg gat Trp Asp cct gat Pro Asp aag cgc Lys Arg aat gct Asn Ala gtg atc Val Ile 100 tac gta Tyr Val ggt gtt Gly Val cca gct Pro Ala cga ata Arq Ile 165 aac acg Asn Thr 180 gca ggc Ala Gly 115 163 211 259 307 355 403 451 499 547 595 643 691 739 ttg agc aca ctc tcg agg gaa ctg aaa agg gac gtt tca gtt gag gaa Leu Ser Thr Leu Ser Arg Glu Leu Lys Arg Asp Val Ser Val Giu Glu WO 01/00805 tta gte gag cc Leu Val Glu Pi 215 etg gtt gtt tc Leu Val Val SE 230 aat etc cct a2 Asn Leu Pro L,, t cg Ser ga t Asp cgg Arg 250 PCTABOO/00926 ate cgc gca ttg gat gat get ttg get ggt egg 787 Ile Arg Ala Leu Asp Asp Ala Leu Ala Gly Arg 220 225 eat tet tte gge age geg eec gae eea act aag .835 His Ser Phe Gly Ser Ala Pro Asp Pro Thr Lys 235 240 245 ggg tagtaegagg aattttgteg gtg 876 Gly <210> 188 <211> 251 <212> PRT <213> Corynebaeterium giutamieum <400> 186 Met Thr Ala Pro Arg Asp Pro Phe Phe 1 Ala Gin Asp Th r Pro Gly ValI Gly Val1 145 Ile Asn Ile Val1 Ser Glu Glu Al a Val1 Gin Val1 Asp 130 Trp Gly Cys Ala Ser 210 Al a Al a Ile Gly Ile Leu Asp 115 Met Val1 Ile Asn Asp 195 ValI Giu Trp Pro Lys Asn Val1 100 T yr Gi y Pro Arg Asn 180 Ala Glu 5 Pro Asp Asp Arg Al a Ile Val Val1 Ala Ile 165 Thr Gly Glu Ile Tyr Gin Thr 70 Asp Tyr Arg Ala His 150 Thr Leu Leu Leu Glu Gin Le u 55 Gin Arg Pro Arg Gi y 135 Asp Arg Asp Ser Val1 215 Ile Al a 40 Le u Pro Gly Ile Leu 120 Ala Gi y Gly Phe Th r 200 Glu Gin 25 Giu Ile Giu Gly Ile 105 Glu GI y Trp Val Tyr 185 Le u Pro Pro 10 Arg Le u Le u Asp Arg 90 Ly s Glu Ar g Val1 Ala 170 Gi u Se r Se r Ala Leu Ala Glu Le u 75 Ile Leu Al a Ile Asp 155 Met His Arg Ile Asp Gi y Th r His Pro Th r Al a Leu Asp 140 Se r His Ile Giu Arg 220 Le u Le u Arg Pro Th r Trp Asp Ile 125 Gly Lys Gly Ile Le u 205 Ala Ser Ile Arg Ser Asn His Pro 110 Gin Arg Val1 Val1 Pro .190 Ly s Leu Ile Arg Asp Tyr Ala Asn Val Tyr Gly Leu Gly Pro Ile Asp Val Val Ser Gly Ala Ala 160 Ala Ile 175 Cys Gly Arq Asp Asp Asp WO 01/00805 PCTIBOO/00926 Ala Leu Ala Gly Arg Leu Val Val Ser Asp His Ser Phe Gly Ser Ala 225 230 235 240 Pro Asp Pro Thr Lys Asn Leu Pro Lys Arg Gly 245 250 <210> 189 <211> 1167 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1144) <223> RXS01747 <400> 189 cgaagtagag ccgattqcag aatcggcgga atgagacgtc ctaaaaatat cactaactcg aaagatgtaa ggttgcattt gaaaaqcgtt taagctttcc gaa Gi u att Ile gag Glu gtg Val1 cgt Arg ga t Asp gag Glu t cc Ser tgg Trp acc gga Gly gag Glu tat Tyr tg t Cys gag Giu ttc Phe cca Pro acc Th r ctg Leu 135 ggt cga Arg a cg Thr cag Gin cag Gin gca Ala t gc Cys ctg Leu atc Ile 120 t ac Tyr gtg cga Arg aag Lys ga t Asp gag Glu acc Th r atg Met cgt Arg 105 acc Th r t ca Ser gaa ctg Leu cct Pro atg Met gct Ala ttc Phe atc Ile gt c Val1 ggt Gly gaa Glu aac ct a Le u cga Arg aag Lys ggc Gly ct C Leu 75 aac As n gct Ala gtt Val gtg Val1 ctg cgc Ar9 tgg Trp gaa Glu t gt Cys 60 att Ile t cg S er gag Glu acc Thr gtt Val1 140 gtg gtC Val1 att Ile cgt Arg 45 cct Pro ggt Gly gct Ala t Ct Ser cg t Arg 125 cg t Arg Cct gaa Glu a ga Arg 30 gtc Val aa t Asn ggc Gi y cgc Arg gtt Val1 110 gat Asp aag Lys ga t gct Ala 15 aac Asn gct Al a atc Ile gcc Ala cct Pro 95 cgt Arg ga t Asp atc Ile ttC cga Arg cag Gin Gly cat His aac Asn 80 gag Gl u gag Glu ct g Le u ca C His tCC gtg Val1 1 aac Asn gtc Val1 g ca Ala gag Glu t gc Cys cca Pro atg Met gat Asp gag Glu 145 ggc tca Ser aaa L ys tca Ser tgt Cys t ct Se r ctc Leu cag Gin ga t Asp 130 ctg Leu aag gaa Glu aac As n cta Le u t gg Trp cgc Arg gac Asp ctg Leu 115 gaa Glu aac Asn aag acc Thr gga Gly cac His gaa Glu cgc Arg cgc Arg 100 aat Asn ggc Gly cca Pro gat ccg Pro cct Pro act Th r t cc Ser tgt Cys gg t Gi y ta c Tyr gca Al a cac His ct g act atc gca cct Thr Ile Ala Pro 115 163 211 259 307 355 403 451 499 547 595 WO 01/00805 WO 0100805PCTA~BOO/00926 Thr Giy Vai Giu Asn Leu Val Pro Asp Phe Ser Gly Lys Lys Asp Leu 150 ctg Leu ga a Giu gag Giu acc Thr acc Th r 230 atc Ile tgg Trp atg Met cgt Arg atc Ile 310 gcc Ala acc Thr cag Gin act Th r cg t Arg aag Ly s 215 gag Giu acc Thr gtg Val1 Gi y gca Ala 295 cca Pro cag Gin cca Pro gaa Gi u gt g Val1 tca Se r 200 tcc Se r g cg Ala ca g Gin aag Lys ttc Phe 280 ggc Gi y gca Ala ga a Giu gtg Val 155 gtt ttt gaa Vai Phe Giu 170 cca cgt att Pro Arg Ile 185 ctt gat gtg Leu Asp Vai aac ctg att Asn Leu Ile ctg cag gat Leu Gin Asp 235 tac ctg cgt Tyr Leu Arg 250 cct gag gag Pro Giu Glu 265 gct gct gtt Ala Aia Vai cgt ctq tac Arg Leu Tyr cac ctc gcg His Leu Ala 315 gca tct aca Ala Ser Thr 330 tcc cgc cca gag Ser Arg Pro Giu 175 ttc aag cgc att Phe Lys Arg Ile 190 atc cgt cag gct Ile Arg Gin Ala 205 ttg ggc atg ggt Leu Giy Met Gly 220 ctg cac gac gct Leu His Asp Ala cct ggt cct ttg Pro Gly Pro Leu 255 ttc ctc gag cac Phe Leu Glu His 270 atg tcc ggc cca Met Ser Gly Pro 285 gcg cag gcc atg Ala Gin Aia Met 300 cac ctg aag gat His Leu Lys Asp ctt ctg gag cgt Leu Leu Giu Arg 335 160 gtt Val1 cgc Arg cg c Arg ga a Giu gg C Gi y 240 ttc Phe gct Aia ttg Leu gag Giu act Th r 320 tac ttc Ph e cc a Pro ga t Asp acc Thr 225 tgt Cys cac His gat Asp gtt Val ttc Phe 305 t cc Se r gg t gct Ala g ca Ala ttc Phe 210 aag Lys ga c Asp ccc Pro gct Ala cgt Arg 290 cgt Arg gg a Gly gct ca c His ttc Phe 195 ggt Gi y ga a Glu atc Ile atc Ile gca Aila 275 tcc Se r ggc Giy gga Gly tcC aac Asn 180 cgc Arg ct g Leu gaa Gi u atc Ile gag Glu 260 a ag Lys t ct Ser gag Giu tcc Ser ga a Giu 340 gtg Val tac Tyr gt g Val atc Ile acc Thr 245 cgt Arg gaa Gi u ta c Tyr gaa Glu acc Thr 325 gac Asp 643 691 739 787 835 883 931 979 1027 1075 1123 1167 Tyr Giy Aia Ser gtg Val 345 tcc ttc aac Ser Phe Asn taagcccgaa gttttttaac cgc (210> 190 <211> 348 <212> PRT '<213> Corynebacterium giutamicum <400> 190 Val Thr Ile Ala Pro Glu Giy Arg Arg Leu Leu Arg Val Giu Ala Arg 1 5 10 .WO 01/00805 Asn Ser Giu Thr Pro Ile Giu Thr PCTJIBOO/00926 Asn Gin Val1 Al a Giu Cys Pro Met Asp Glu 145 Gly Phe Pro Asp Thr 225 Cys His Asp Val Phe 305 Ser Gly Lys Ser Cys Ser Le u Gin Asp 130 Leu Lys Ala Ala Phe 210 Lys Asp Pro Al a Arg 290 Arg Gly Ala Asn Leu Trp Arg Asp Le u 115 Glu Asn Lys His Phe 195 Gly Glu Ile Ile Ala 275 Ser Gly Gly Se r Pro Thr Ser Cys Giy T yr Ala His Leu 165 Val1 Tyr Val1 Ile Thr 245 Arg Giu Tyr Glu Th r 325 Asp Glu Val1 Arg 70 Asp Giu Se r Trp Th r 150 Leu Glu Giu Th r Thr 230 Ile Trp Met Arg Ile 310 Al a Th r Lys Asp Giu Th r Met Arg 105 Thr Se r Giu Val1 Pro 185 Le u Asn Le u Tyr Pro 265 Al a Arg His Ala ValI Pro Arg Trp Ile Lys Giy Le u 75 As n Ala Val1 Val1 Leu 155 Gi u Ilie ValI Ilie Asp 235 Arg Gi u Val1 Tyr Al a 315 Th r Phe Glu Cys Ile Ser Glu Thr ValI 140 Vai Ser Phe Ile Le u 220 Leu Pro Phe Met Ala 300 His Leu Asn Arg Pro Gly Ala S er Arg 125 Arg Pro Arg Lys Arg 205 Gl y His Gi y Le u Ser 285 Gin Leu Leu Gi y His Asn Glu Giu Leu His Se r 160 Vai Arq Arg GI u Gi y 240 Phe Ala Leu Giu Th r 320 Tyr WO 01/00805 WO 0100805PCT/IBOO/00926 <210> 191 <211> 903 <212> DNA <213> Corynebacteriun glutamioum <220> <221> CDS <222> (101)..(880) <223> RXC01748 <400> 191 atcaocaaat gtggoggttt tgogtcgaaa agcgtgctct ttctacaoot ctttgaggtt cattttcgcg gtttoctcac aatcgoctat tgttaagtac atg gca gao gcg aaa Met Ala Asp Ala Lys cag Gin cag Gin Cgo Arg gg t Giy t gg Trp ttt Ph e gg t Gly gtg Val atg Met 135 gg o Gly aag aaa Lys gag Glu gat Asp ctg Le u ctt Leu acc Thr gg t Gly aco Thr gt g Val1 too Se r aac 900 Ala cgo Arg gc t Al a ott Leu ato 'le ogo Arg got Ala cg 0 Ar g cgo Arg 140 cac His gt g aag L ys t ca Ser ott Leu tto Phe ggc Gi y gtt Val1 ggt Gly aco Thr 125 gtc Val1 cgc Arg gca aag Ly s oaa Gin att Ile ct 0 Le u att Ile gag Gi u tgg Trp 110 a ag Ly s att Ile ctg Le u cot cag Gin 15 atg Met cog Pro atc Ile got Al a cg t Arg 95 gct Aia aco Thr ggt Giy aag Lys gg t aag Ly s aac As n got Al a ggt Gly got Ala cg c Arg 100 ogc Arg aco Th r gt g Val1 ca g Gin gaa aag Lys atg Met att Ile ggt Gly gca Ala gco Ala tot Ser act Th r o tg Leu caa Gin 165 ato WO 01/00805 Lys Lys Arg Leu Asn Arg Val Ala Pro 170 atc acg ggc aac ggc gaa ggc cag acc Ile Thr Gly Asn Gly Giu Gly Gin Thr 185 190 gaa ctg gtc aag ctg cct cgc aac tac Giu Leu Val Lys Leu Pro Arg Asn Tyr 200 205 ctg gcc gct cgc att gag gct atg gac Leu Ala Ala Arg Ile Giu Ala Met Asp 215 220 gga tct ttg cct aag ggt cca ttg cca Gly Ser Leu Pro Lys Giy Pro Leu Pro 230 235 atg aac cgc cgc gct cgc cga cag gct Met Asn Arg Arg Ala Arg Arg Gin Ala 250 taaagccttt tcgctttcgc gtc <210> 192 <211> 260 <212> PRT <213> Corynebacterium giutamicum <400> 192 Met Ala Asp Ala Lys Lys Gin Ala Asp Gi y 175 cct Pro aag Lys aat Asn aa g Lys ga a Glu 255 Pro gcg Ala aac Asn gga Gi y 225 g ca Ala aag Lys Val1 aag Lys gac Asp 210 aac Asn a gc Ser ggc Gly T yr ctgq Le u 195 gtC Val gct Al a atg Met gag Glu Glu 180 cag Gin gct Al a cct Pro tcc Se r gct Ala 260 PCTLBOO/00926 Ile cgt 691 Arg gcc 739 Ala ggc 787 Gi y ggt 835 Gly 245 880 1 Arg Gin Leu Leu Gly Val1 Giu Val Cys 145 Al a Val1 Leu Ile Val1 Tyr As n Ala 130 Gly Ala Phe Leu Trp Val L ys Leu 115 Val1 Val Gin Arg Gly T rp Ph e Gly ValI Met 135 Gly Arg Lys Ile Trp Ile Gin Gi y 120 Asp Glu Lys Gin Pro Met Phe Gin 105 Met Al a Gi y Lys 10 Giu Asp Leu Le u Thr Giy Th r Val1 Ser Lys Gin Ile Leu Ile Gi u Trp 110 Ly s Ile Le u Gin Met Pro Ile Ala Arg Ala Th r Gly Lys ValI Trp Leu Gi y Ala Asp Val1 Ala Leu Pro 160 Met Pro Ala Asn Gi y 225 Ala Lys WO 01/00805 Leu Ala G.

Val Tyr G: 1 Lys Leu G: 195 Asp Val A: 210 Asn Ala Pi Ser Met S( Gly Glu A] ln lu 80 In La La Ly s Ile Glu Leu Gly 230 Met Asn 170 Gi y Leu Ile Lys Ala 250 Arg Glu Pro Gi u Gly 235 Arg Pro Thr 190 Tyr Asp Pro Ala Gly 175 Pro Lys Asn Lys Glu 255 PCT/tBOO/00926 Val1 Ile Ly s Val1 Gly 240 Arg <210> 193 <211> 936 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(913) <223> RXC00354 <400> 193 ggcttgtcqq taaggctgca gggtagcggg agtttccttc ttctcgatta tgtacctcat ttacgcgtaa agtttqgggc tt c Phe cg t Arg gt g Val1 tgg Trp ga a Glu acc atc Ile gt t Val agc Ser gg a Gly ca g Gin att gg t Gly gac Asp tct Ser gtg Val gag Glu gtg aca Th r atc Ile cga Arg ggc Gi y gtg Val1 t gg agattggaag ttctttaatt atg gga aag tta ctt Met Gly Lys Leu Leu 1 tca aat gaa gtt ccg Ser Asn Glu Val Pro gcc aaa gga cac cgc Ala Lys Gly His Arg gtg act tct cag ttg Val Thr Ser Gin Leu aat ggt gga tat gtg Asn Gly Gly Tyr Val ttg tcg ggt gag gag Leu Ser Gly Giu Glu ggt ttg gag ttt tat WO 01/00805 WO 0100805PCTIBOO/00926 Thr Arg His Ile Vai Giu Trp Leu Tyr Asn Arg Gly Leu Glu Phe Tyr 95 100 etc gag tcc aac aae ggt ttg tat gca age cgt ggt tte cgt gag gct -451 Leu Giu Ser Asn Asn Gly Leu Tyr Ala-Ser Arg Gly Phe Arg Giu Ala 105 110 115 tet aag cca gtg etg tct cge ctt teg gag aag aec gac gtg aca gtc 499 Ser Lys Pro Val Leu Ser Arg Leu Ser Glu Lys Thr Asp Vai Thr.Vai 120 125 130 gat age atg tac ccg gat atg ttc tgg ggc gcg age ctt gat cgt gac 547 Asp Ser Met Tyr Pro Asp Met Phe Trp Gly Ala Ser Leu Asp Arg Asp 135 140 145 gat gtg aac aag atc agt tac atc ttc aat tet eag gaa gat ttg gac 595 Asp Val Asn Lys Ile Ser Tyr Ile Phe Asn Ser Gin Glu Asp Leu Asp 150 155 160 165 gca gcg cgt gag geg tte cet aac ctg gag cac acc acg tgg ggt ggt 643 Ala Ala Arg Giu Ala Phe Pro Asn Leu Giu His Thr Thr Trp Gly Gly 170 175 180 eag aeg ggt gcg ttg tte ggc acg ate ggt gtg tct gte aac aag aag 691 Gin Thr Gly Ala Leu Phe Gly Thr Ile Gly Val Ser Val Asn Lys Lys 185 190 195 atc ggc gtg gat cgc etg ctg aag tac etg aae gca gat cgc gca aac 739 Ile Giy Val Asp Arg Leu Leu Lys Tyr Leu Asn Ala Asp Arg Ala Asn 200 205 210 ace att geg tte ggc gac age gat gag gat etc tee cta ttt gag gcg 787 Thr Ilie Ala Phe Gly Asp Ser Asp Giu Asp Leu Ser Leu Phe Glu Ala 215 220 225 age get tac ggc gte gcg atg gge gag gcc acc gaa tcg ctc aag get 835 Ser Ala Tyr Gly Val Ala Met Gly Giu Ala Thr Giu Ser Leu Lys Ala 230 235 240 245 get gct gac ctg gte acg gat get gtt ggg cag gac gge ttg cgc aat 883 Ala Ala Asp Leu Val Thr Asp Ala Val Gly Gin Asp Gly Leu Arg Asn 250 255 260 gcg ttt tta aag ett gag ctt atc gac gee tgaecccate aaagaaettc 933 Ala Phe Leu Lys Leu Giu Leu Ile Asp Ala 265 270 cca 936 <210> 194 <211> 271 <212> PRT <213> Corynebaeterium giutamieum <400> 194 Met Gly Lys Leu Leu Phe Val Asp Ile Gly Gly Thr Leu Leu Asp Tyr 1 5 10 Ser Asn Giu Val Pro Arg Ser Ala Val Asp Ala Ile Arg Lys Ala Arg 25 WO 01/00805 PCTJLBOOIOO926 Ser Ser Gly Arg Ser Ser Ala Glu Al a Val1 As n Leu Gly Gly Thr Ser 145 Gln Thr Se r Ala Ser 225 Glu Asp Lys Th r Gi y Ser Leu Ph e Asp 130 Leu Glu Thr Val1 Asp 210 Leu Se r Gly Gly Ser Gly Gly Glu Arg 115 Val1 Asp Asp T rp As n 19.5 Arg Phe Leu Le u His Gin Tyr Giu Phe 100 Giu Thr Arg Leu Gly 180 Lys Ala Glu Lys Arg 260 Arg Val Tyr Leu 40 Leu Trp Val Glu 70 Giu Thr Tyr Leu Ala Ser Val Asp Asp Asp 150 Asp Ala 165 Gly Gln Lys Ile Asn Thr Ala Ser 230 Ala Ala 245 Asn Ala Asp 55 Ser Arg Glu Lys Ser 135 Val1 Ala Thr Gly Ile 215 Ala Ala Phe Ile Ala His Ser Pro 120 Met Asn Arg Gly Val1 200 Ala Tyr Asp Leu Gly Gin Ile Asn 105 Val T yr Lys Glu Al a 185 Asp Phe Gly Le u Ly s 265 Val1 Glu Val1 90 Asn Leu Pro Ile Ala 170 Leu Arg Gly Val1 Val 250 Leu Asp Ser Glu Gly Ser Asp Ser 155 Phe Phe Leu Asp Ala 235 Th r Glu Gly Val Trp Leu Arg Met 140 Tyr Pro Gly Le u Ser 220 Met Asp Leu Leu Ph e Leu Tyr Le u 125 Phe Ile As n Thr Lys 205 Asp Gi y Al a Ile Ile His T yr Al a 110 Ser Trp Phe Leu Ile 190 Tyr Gi u Glu Val1 Asp 270 Gly Arg Asnr S er Glu Gly Asn Giu 175 Gly Leu Asp Al a Gly 255 Al a <210> 195 <211> 1740 <212> DNA <213> Corynebacterium glutamicum <22 0> <221> CDS <222> (1717) <223> RXC01749 <400> 195 ggtttttgcg tgctctggtt tagggactgg ttttgggaac qtgcccagtt ccacatcaaa taacgctgjag gtcgtactta atccatgaga tcatqaatgg gtg aqc ttc ctt gta 115 WO 01/00805 WO 0100805PCTIBOO/00926 ga a Giu ctc Leu ctg Le u cca Pro gg t Gly ctg Le u ct C Leu gcc Ala gtt Val1 ctc Leu 150 gg t Gly a aa Lys ga a Glu ctg Leu gtg Val aat As n ggC Gly ttt Phe tcc Se r Ct 9 Le u cgc Arg gca Ala ggC Gly gt t Val1 135 atc Ile gtg Val gtc ValI Ct C Le u gag Glu 215 gaa Gi u caa Gin cg c Arg gta Val1 ctc Leu gaa Glu aac Asn tgg Trp atg Met 120 gac Asp gcc Al a ctc Leu gac Asp aaa Lys 200 aac Asn cga Arg tta Leu atc Ile ggt Gly att Ile gcc Ala aac Asn gca Ala 105 ctc Le u gca Ala gag Gi u atc Ile cac His 185 ggc Gly atc Ile qac Asp ctc Leu aaa Lys cta Leu tac T yr gqC Gi y gca Al a ct C Leu acc Thr ctt Leu ctg L eu gtt Val1 170 aac Asn cgt Arg cca Pro 99 t Gly g cg Al a att Ile g cg Ala gtg Vali cct Pro 75 ctg Le u atc Ile Gi y cct Pro

CCC

Pro 155 att Ile aaa Lys cgc Arg gag Glu gag Glu gtt Vai ggg Gly tcc Ser 45 gg a Gly ttc Phe t tg Leu gtt Val ctC Leu 125 ctt Leu gtc Val1 tcc Ser gcc Aia cgc Arg 205 ctc Le u ttc Phe gtc Val1 ttc Ph e 30 acc Th r ct g Leu ttc Phe ggc Gly ttg Le u 110 acc Thr atc Ile gca Al a atc Ile ga a Glu 190 gt C Val1 aac Asn atc Ile atc Ile 15 cgt Arg att Ile t cg S er a cc Thr gcc Ala 95 aac Asn aac Asn ga c Asp tat T yr gcc Ala 175 gaa Giu acc Thr CtC Leu ccg Pro atg Met Ctc Le u gag Giu Ctt Leu tcc Se r 80 atc Ile atc Ile a cc Thr gac Asp t CC Se r 160 atc Ile g cg Al a gtc Val cac His ctt Le u Vai 1 acg Th r ggc Gly ccg Pro ttt Ph e atg Met atc Ile ga t Asp cca Pro a cc Thr 145 t tg Leu ttc Ph e ggc Gly gCt Ala gtc Val1 225 tat Tyr Ser gtC Val1 gt c Val1 gat Asp gtc Val aaa Lys gcc Ala gc Ala gcc Ala 130 ggC Gly gca Ala agc Se r gtt Val ga t Asp 210 att Ile 99 C Gly Ph e gga Gi y gcc Al a att Ile tac Tyr acc Thr acc Thr gcc Al a 115 a tg Met ca g Gin tac Tyr tct Ser g cg Al a 195 ctt Leu gtg Val1 gaa Glu Le u cta Leu gct Ala tcc Ser acg Th r act Th r acg Th r 100 tcc Ser gc C Ala ctt Le u ccc Pro gtg Val1 180 gt c Val1 cca Pro tcc Ser cac His Val ttg Leu gta Val gt c Val1 atc Ile ggt Gl y gca Ala ggC Gi y g ca Al a cac His CtC Leu 165 ttC Phe c ag Gin gcc Ala cga Arg gca Ala 163 211 259 307 355 403 451 499 547 595 643 691 739 787 835 WO 01/00805 PCTJIBOO/00926 230 235 240 245 cgc atc ggc gat gtc tta aca gtg gtg ggt gcc gat gaa gaa ctc aac 883 Arg Ile Gly Asp Val Leu Thr Val Val Gly Ala Asp Giu Giu Leu Asn 250 255 260 cgc gcg gaa aaa gcc atc ggt gaa ctc att gac qgc gac ccc tac agc 931 Arg Ala Giu Lys AlaIlie Gly Giu Leu Ile Asp Gly Asp Pro Tyr Ser 265 270 275 aat gtg gaa ctt gat tac cga cgc atc ttc gtc tca aac aca gca gtc 979 Asn Val Giu Leu Asp Tyr Arg Arg Ile Phe Val Ser Asn Thr Ala Val 280 285 290 gtg ggc act ccc cta tcc aag ctc cag cca ctg ttt aaa gac atg ctg 1027 Val Gly Thr Pro Leu Ser Lys Leu Gin Pro Leu Phe Lys Asp Met Leu 295 300 305 atc acc cgc atc agg cgc ggc gac aca gat ttg gtg gcc tcc tcc gac 1075 Ile Thr Arg Ile Arg Arg Gly Asp Thr Asp Leu Val Ala Ser Ser Asp 310 315 320 325 atg act ttg cag ctc ggt gac cgt gtc cgc gtt gtc gca cca gca gaa 1123 Met Thr Leu Gin Leu Gly Asp Arg Val .Arg Val Val Ala Pro Ala Giu 330 335 340 aaa ctc cgc gaa gca acc caa ttg ctc ggc gat tcc tac aag aaa ctc 1171 Lys Leu Arg Giu Aia Thr Gin Leu Leu Giy Asp Ser Tyr Lys Lys Leu 345 350 355 tcc gat ttc aac ctg ctc cca ctc gct gcc ggc ctc atg atc ggt gtg 1219 Ser Asp Phe Asn Leu Leu Pro Leu Ala Ala Gly Leu Met Ile Giy Val 360 365 370 ctt gtc ggc atg gtg gag ttc cca cta cca ggt gga agc tcc ctg aaa 1267 Leu Val Gly Met Val Glu Phe Pro Leu Pro Gly Gly Ser Ser Leu Lys 375 380 385 ctg ggt aac gca ggt gga ccg cta gtt gtt gcg ctg ctg ctc ggc atg 1315 Leu Gly Asn Ala Giy Gly Pro Leu Val Vai Ala Leu Leu Leu Gly Met 390 395 400 405 atc aat cgc aca ggc aag ttc gtc tgg caa atc ccc tac gga gca aac 1363 Ile Asn Arg Thr Gly Lys Phe Val Trp Gin Ile Pro Tyr Gly Aia Asn 410 415 420 ctt gcc ctt cgc caa ctg ggc atc aca cta ttt ttg gct gcc atc ggt 1411 Leu Ala Leu Arg Gin Leu Giy Ile Thr Leu Phe Leu Ala Ala Ile Gly 425 430 435 acc tca gcg ggc gca gga ttt cga tca gcg atc agc gac ccc caa tca 1459 Thr Ser Ala Gly Ala Gly Phe Arg Ser Ala Ile Ser Asp Pro Gin Ser 440 445 450 ctc acc atc atc ggc ttc ggt gcg ctg ctc act ttg ttc atc tcc atc 1507 Leu Thr Ile Ile Gly Phe Giy Ala Leu Leu Thr Leu Phe Ile Ser Ile 455 460 465 acg gtg ctg ttc gtt ggc cac aaa ctg atg aaa atc ccc ttc ggt gaa 1555 Thr Val Leu Phe Val Gly Hi-s Lys Leu Met Lys Ile Pro Phe Gly Giu 470 475 480 485 WO 01/00805 PCTIBOOIOO926 acc gct ggc atc ctc gcc ggt acg caa acc cac cct gct gtg ctg agt 1603 Thr Ala Gly Ile Leu Ala Gly Thr Gin Thr His Pro Ala Val Leu Ser 490 495 500 tat gtg tca gat gcc tcc cgc aac gag ctc cct gcc atg ggt tat acc 1651 Tyr Val Ser Asp Ala Ser Arg Asn Giu Leu Pro Ala Met Gly Tyr Thr 505 510 515 tct gtg tat ccg ctg gcg atg atc gca aaq atc ctg gcc gcc caa acg 1699 Ser Val Tyr Pro Leu Ala Met Ile Ala Lys Ile Leu Ala Ala Gin Thr 520 525 530 ttg ttq ttc cta ctt atc tagcattgac cccttaagcg cag 1740 Leu Leu Phe Leu Leu Ile 535 <210> 196 <211> 539 <212> PRT <213> Corynebacterium giutamicum <400> 196 Val Ser Phe Leu Val Giu Asn Gin Leu 1 Th r Gi y Pro Phe Met Ilie Asp Pro Thr 145 Leu Phe Gly Val1 Val Asp Val1 Lys Al a Ala Ala 130 Gi y Ala Se r Val1 Gly Ala Ile Tyr Thr Thr Ala 115 Met Gin Tyr Ser Al a Le u Al a Ser Th r Th r Th r 100 Ser Al a Le u Pro Val1 180 ValI Leu Val1 Val1 Ile Gly Ala Gly Ala His Leu 165 Phe Gin Le u Leu Pro Gly Leu Le u Ala Val1 Leu 150 Gly Lys Glu Le u Phe Leu Val1 Gi y Thr Th r Al a Ser 140 Val1 Leu Giu Ile Val1 Gi y Se r Gly Phe Leu Val Le u 125 Leu ValI Ser Ala Arg WO 01/00805 195 PCTfIIBOO/00926 Asp 210 Ile Gly Giu Asp As n 290 Lys Ala Ala Tyr Met 370 Ser Le u Tyr Ala Asp 450 Phe Pro Ala Met Le u Va 1 Giu Giu Pro 275 Thr Asp Ser Pro Lys 355 Ile Ser Leu Gly Ala 435 Pro Ile Phe Val1 Gly 515 Pro Ser His Le u 260 Tyr Al a Met Se r Al a 340 Ly s Gly Leu Gly Al a 420 Ile Gin Ser Gi y Leu 500 Tyr Al a Arg Al a 245 As n Ser Val1 Le u Asp 325 Giu Leu Val1 Lys Met 405 Asn Gly Ser Ile Glu 465 Se r Thr Glu 215 Giu Ile Ala Val1 Gly 295 Th r Tb r Le u Asp ValI 375 Gly As n Al a Se r Thr 455 Val1 Ala Val1 Val1 As n Arg Gi y Giu Giu 280 Th r Arg Leu Arg Ph e 360 Gly Asn Arg Leu Ala 440 Ile Leu Gly Ser T yr 520 Ile Asp Asp Lys 265 Leu Pro Ile Gin Glu 345 As n Met Al a Tb r Arg 425 Gi y Ile Ph e Ile Asp 505 Pro Pro Gly Val1 250 Ala Asp Leu Arg Le u 330 Ala Leu Val1 GI y GI y 410 Gln Al a GI y Val1 Le u 490 Al a Le u Glu Giu 235 Leu Ile Tyr Ser Arg 315 Gly Thr Leu Glu Gi y 395 L ys Le u Gly Phe Gly 475 Al a Se r Al a Leu 220 Gin Thr Gly Arg Ly s 300 Gi y Asp Gin Pro Ph e 380 Pro Phe Gly Phe Gly 460 His Gly Arg Met Le u Phe Val1 Glu Arg 285 Leu Asp A r c Le u Leu 365 Pro Leu Val1 Ile Arg 445 Ala Lys Th r Asn Ile 525 Leu Pro Gi y 255 Ile Phe Pro Asp Arg 335 Gly Ala Pro Val1 Gin 415 Leu Ala L eu Met Th r 495 Leu Lys His Leu 240 Al a Asp Val1 Leu Leu 320 Val Asp Gly GlIy Ala 400 Ile Phe Ile Thr Lys 480 His Pro Ile WO 01/00805 Leu Ala Ala Gin Thr Leu Leu Phe Leu Leu Ile 530 535 <210> 197 <211> 1023 <212> DNA <213> Corynebacterium qiutamicum <220> <221> COS <222> (101)..(1000) <223> RXA02268 <400> 197 tgaatcgttg gtgcttcgag ttgggattgt tatgtgggga PCTIBOO/00926 gacgtcgata agcaaaacac ttgccgagcg caagccggcc tacggcgcta gtgtgagcac atg tcc cag gaa aat Met Ser Gin Giu Asn tct Ser cg g Arg gat Asp aat Asn c cg Pro t gg Trp gcc Ala gcc Aia g cg Aila tat Tyr 150 ggt Gly aat Asn cac His ggg Gly t gg Trp gag Giu cct Pro caa Gin ca g Gin 135 tgg Trp ttg Leu ccg Pro gca Aia ttt Phe ccg Pro gaa Giu ga c Asp att Ile 120 att Ile atg Met a ca Thr gag Giu ccc Pro C99 Arg gga Giy cgc Arg acc Thr 110 g cc Ala t cg Ser tac Tyr cg t Arg gaa Giu ctg Leu gcg Ala t cc Ser agc Ser gag Giu gt t ValI caa Gin atg Met ggg Gly ttc Phe acg Th r cga Arg g gg Giy 80 aaa Lys cca Pro ttg Le u ggc Gly aag Lys 160 1 gtg ValI acc Th r gcg Aila cca Pro gcc Ala ggt Giy att Ile ttg Leu ggt Gly 145 gt c Val cgc Arg tac Tyr ccg Pro cg a Arg tca Ser ttt Ph e gct Al a gc t Al a cgg Arg att Ile 165 115 163 211 259 307 355 403 451 499 547 595 WO 01/00805 WO 0100805PCT/IBOO/00926 tcc atc tct acg cca aat cac gga acg Ser Ile Ser Thr Pro Asn His Gly Thr 170 ccg atg acg aag gtg aaa tcg gga gag Pro Met Thr Lys Val Lys Ser Gly Glu 185 190 cac aga cta ttc ggg ccc act ggt ttt His Arg Leu Phe Gly Pro Thr Gly Phe 200 205 atc atc gag ttt ttg gcc gac ggt ggg Ile Ile Giu Phe Leu Ala Asp Gly Gly 215 220 tac acc tgc att ggt acc cat ttt gat Tyr Thr Cys Ile Gly Thr His Phe Asp 230 235 gtg gcc ttt ttg gag gtc aac gag gac Val Ala Phe Leu Glu Val Asn Giu Asp 250 aat att tgg gtc gaa gat gaa cac ccg Asn Ile Trp Val Glu Asp Glu His Pro 265 270 gat atg gtg cgc gat ccc agg gtg atc Asp Met Val Arg Asp Pro Arg Vai Ile 280 285 gac cgg gtg gca cgc ctc ggc taagttg Asp Arg Val Aia Arg Leu Gly 295 300 <210> 198 <211> 300 <212> PRT <213> Corynebacterium giutamicum <400> 198 Met Ser Gin Giu Asn Ser Gly Leu Phe 1 Vai Ala Lys Val Arg Arg Asn Pro Arg Thr Gin Giu Leu Tyr Asp His Ala Thr 40 Ala Arg Leu Lys Pro Asn Gly Phe Phe 55 Pro Ser Gly Ala Arg Pro Trp Pro Ile Ala Ser Lys Gly Ser Trp Giu Giu Met ctc atg Leu 175 gg a Gi y gaa Giu ga c Asp cct Pro ga t Asp 255 cg c Ar g ga a Gi u Met a cg Th r cag Gin ctc Leu ttc Ph e 240 gat Asp g ca Aia atc gga Giy atc Ile ctg Leu ga t Asp 225 atc Ile c ca Pro atg Met gta ggc Giy gaa Giu cgc Arg 210 cca Pro caa Gin aat Asn att Ile cg c att Ile aag Lys 195 gga Gly ggc Gly cct Pro cga Arg g cc Ala 275 gca tta Leu 180 ctg Leu cac His gt c Val ccg Pro gt c ValI 260 cac His gag Glu 643 691 739 787 835 883 931 979 1023 Ile Val Arg Ala ggg acatggttga cgc Ala Phe Thr Asp Ile Glu Ile Ala Le u Trp His Le u WO 01/00805 PCTIBOO/00926 Gly Trp Ala Val Phe Ala Pro Asp Phe Gly Thr Arg Ala Thr Giu Pro 100 105 110 Ile Ala Ala Ser Ala Ala Gin Ile Gly Ala Tyr Ile Asp Ala Val Leu 115 120 125 Leu Val Thr Gly Ala Ala Gin Ile Val Leu Val Gly His Ser Gin Gly 130 135 140 Gly Val Val Ala Arg Tyr Trp Met Arq Thr Tyr Gly Gly Tyr Met Lys 145 150 155 160 Vai Arg His Met Ile Ser Ile Ser Thr Pro Asn His Gly Thr Leu Met 165 170 175 Giy Gly Ile Leu Asn Pro Met Thr Lys Val Lys Ser Gly Giu Gly Thr 180 185 190 Ile Glu Lys Leu Met His Arg Leu Phe Gly Pro Thr Gly Phe Giu Gin 195 200 205 Leu Arg Gly His Asp Ile Ile Glu Phe Leu Ala Asp Giy Gly Asp Leu 210 215 220 Asp Pro Gly Val Thr Tyr Thr Cys Ile Gly Thr His Phe Asp Pro Phe 225 230 235 240 Ile Gin Pro Pro Giu Val Ala Phe Leu Giu Val Asn Giu Asp Asp Asp 245 250 255 Pro Asn Arg Val His Asn Ile Trp Val Giu Asp Giu His Pro Arg Ala 260 265 270 Met Ile Ala His Asn Asp Met Val Arg Asp Pro Arg Val Ile Glu Ile 275 280 285 Val Arg Ala Glu Leu Asp Arg Val Ala Arg Leu Gly 290 295 300 <210> 199 <211> 1095 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1072) <223> RXA02269 <400> 199 cgcaatgatt gcccacaacg atatggtgcg cgatcccagg gtgatcgaaa tcgtacgcgc agagctcgac cgggtggcac gcctcgqcta aqttggggac atg gtt qac gcc ctc 115 Met Val Asp Ala Leu 1 aat gat ctc cgc cga gaa ctc aca aac gcg tta agg tcc gtg tgg aaa 163 Asn Asp Leu Arg Arg Glu Leu Thr Asn Ala Leu Arg Ser Val Trp Lys 15 WO 01/00805 WO 0100805PCTIBOOIOO926 aac ctc ccc act gat aac gcc ccg cag gcc gat gcc ttq cca gac ga t Asp As n gta Val1 Gly ata Ile gaa Glu aaa L ys gca Ala gaa Giu a ca Thr 150 ttg Leu cac His atc Ilie tct Ser gga Gly 230 Leu gtg Val1 aaa Lys cg t Arg ga c Asp ggt Gi y gtg Val1 t ca Ser 135 gga Gi y ctg Leu a tg Met gt c Val1 gtc Val1 215 cac His Pro gaa Glu ctc L eu tca Ser cgc Arg gat Asp ttt Phe 120 tcc S er gcc Ala aga Arg gtc Val agc Ser 200 gt t Val ga c Asp Thr gag Glu aac Asn cgt Arg ccc Pro t gg Trp 105 gca Al a g ca Ala t ca Se r tac Tyr tcc Ser 185 c cg Pro ca a Gin acc Thr Asp Asn Ala Pro Gin Ala Asp Ala Leu Pro Asp 30 aat Asn 45 a ca Thr ga a Glu gta Val gg a Gly ggc Gly 125 gcc Ala gt c Val1 gt t Val ccc Pro aac Asn 205 gg c Gly atc Ile ttc Ph e ga t Asp gac Asp tta Leu gcc Al a 110 caa Gin tat T yr gtt Val1 t tg Leu aat Asn 190 aat Asn gaa Glu aat Asn tac Tyr tcc Ser gat Asp gtc Val1 gat Asp cgc Ar g ata Ile Gi y gg t Gly 175 cac His cgt Arg gct Al a ga a Glu cg t Arg ttg Leu tgg Trp 80 cac His cta Leu gcc Ala ga t Asp cac His 160 gg t Gly gg C Gly ggc Gly gga Gly gg c Gly 240 ga t Asp ccg Pro cgc Arg gga Gi y cgc Arg acc Thr a ca Thr 145 t cc Ser g ca Al a acc Th r gaa Giu ttt Ph e 225 ggc Gi y ccc Pro atg Met gcc Ala act Thr cgc Arg ggt Gly 130 gt a Val1 caa Gin t cc Ser acc Th r agt Ser 210 gaa Glu gat Asp aaa Lys ctc Leu cg c Arg gg a GI y ga c Asp 115 tca Se r ttg Leu ggC Gly aaa Lys atg Met 195 gtg Val1 atg Met ttg Leu aac As n gcg Ala ccc Pro tca Ser 100 ggc Gly gt c Val1 ctt Le u ggc Gly gt c Val1 180 ggc Gly gt t Vai atc Ile ga t Asp cg c Arg cg c Arg acc Th r aca Th r tgg Trp qca Ala gct Ala gtg Val 165 aaa Lys gga Gly aat Asn cg c Arg cca Pro 245 211 259 307 355 403 451 499 547 595 643 691 739 787 835 883 gac gtg aca tat ctg tgc atc gcc acc cac ttt qac acc gtg att cag Asp Val Thr Tyr Leu Cys Ile Ala Thr His Phe Asp Thr Val Ile Gin WOO01/00805 ccc cct gaa acc tgc ttc cta gag gcc cgg Pro Pro Giu Thr Cys Phe Leu Giu Ala Arg 265 270 cgg gtc caa aac atc tgg gtg gaa aac ctc Arg Val Gin Asn Ile Trp Val Giu Asn Leu 280 285 ctc cac gaa gca atg cct tac gat ccc cgc Leu His Giu Ala Met Pro Tyr Asp Pro Arg 295 300 gcg gat ttg agc aaa ttg gtg gag att tcc Ala Asp Leu Ser Lys Leu Val Glu Ile Ser 310 315 taggggtttt ggtggttgtc taa 200 <211> 324 <212> PRT <213> Corynebacterium glutamicum ccc gaa Pro Giu ccc aat Pro Asn 290 cgc gca Arg Ala 305 act gcg Thr Ala PCTIBOOIOO926 gaa ctc aag 931 Giu Leu Lys 275 tca gic gtg 979 Ser Val Val ctg gtc agg 1027 Leu Val Arg gag aac 1072 Giu Asn 1095 <400> 200 Met Val Asp Ala Leu Asn Asp Leu Arg 1 Arg Ala Asp Pro Arg Gly Arg Thr Tb r 145 Ser Al a Ser Le u Pro Met Ala Th r Arg Gly 130 Val1 Gin Ser Val1 Pro Lys Leu Arg Gly Asp 115 Ser Leu Gly Ly s Trp Asp Asn Ala Pro Ser 100 Gi y Val1 Le u Gi y Val1 180 5 Lys Asp Arg Arg Thr Th r T rp Ala Ala Val1 165 Lys Asn Val Gly Ile 70 Giu Lys Ala Giu Th r 150 Leu His Pro Glu 40 Le u Ser Arg Asp Ph e 120 Ser Ala Arg Val1 Th r 25 Glu Asn Arg Pro Trp 105 Ala Ala Ser Tyr Ser 185 Arg 10 Asp Ile Giu Gly T rp 90 Gin Pro Gin Lys Trp 170 Leu Giu Asn Ala Asp Leu 75 Pro Asp Giu Ile Val1 155 Met Ala Le u Al a Ile *Lys Ph e Val1 Leu Phe GI y 140 Ile Arg Val1 Th r Pro As n Tb r Giu Val Gly Gi y 125 Ala Val1 Val Pro As n Gin Ph e Asp Asp Leu Al a 110 Gin Tyr Val Leu As n 190 Ala Ala T yr Ser Asp Val Asp Arg Ile Gi y Gly 175 His Le u Asp Arg Leu Trp His Le u Ala Asp His 160 Gly Gly WO 01/00805 Thr Thr Met Gly 195 Glu Ser Val Val 210 Phe Giu Met Ile 225 Giy Asp Leu Asp Asp Thr Val Ile 260 Pro Glu Glu Leu 275 Pro Asn Ser Val 290 Arg Ala Leu Vai 305 Thr Ala Glu Asn Gly As n Arg Pro 245 Gin Lys Val1 Arg Ile Ser Gly 230 Asp Pro Arg Leu Ala 310 Val1 Val1 215 His Val1 Pro Vai His 295 Asp Ile Trp As n 235 Cys Phe Trp Pro Leu 315 Arg Phe 220 Aia Ile Le u Vali Tyr 300 Vali As n 205 Gi y Ile Ala Giu Glu 285 Asp Giu Asn Giu Asn Th r Al a 270 Asn Pro Ile Arg Aila Glu His 255 Arg Leu Arg Ser PCTAIBOO/00926 Gly Gly Giy 240 Phe Asn Asp Vali Giu 320 <210> 201 <211>. 1146 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1123) <223> RXA01614 <400> 201 tgcaattcgc aaacatgagt agtatcgcgg aagtttcacc tcgcgcacac cacaccaaca atcgacctat cgttatacqt aaa L ys ggC Gly tac T yr t tg Leu ttc Phe ccg Pro ga c Asp gtt Val1 acgtgaacat ttcagtcgac atg aat cag atg cag Met Asn Gln Met Gin 1 caa aac ctc acc atc Gin Asn Leu Thr Ile gtt gtg aca acg gt Vai Val Thr Thr Val tct ttt gct gcc cgc Ser Phe Ala Ala Arg tac ttc ttc cac act Tyr Phe Phe His Thr WO 01/00805 gaa Glu ga t Asp tac Tyr gag Glu act Th r aat Asn 150 tgg Trp atg Met gga Gly gaa Gi u agc Ser 230 ca c His aag Lys gcg Ala t cg Ser gat gag ttt ttc cac aat gcc ggt ttt PCTIBOO/00926 gct gtg tac ggc att 355 Ala Val Tyr Gly Ile Glu Phe Phe 75 aaa tgt gga Lys Cys Gly gat ctt gcc Asp Leu Ala 105 tct tcc acc Ser Ser Thr ctc atc gtt Leu Ilie Val gcc att gag Ala Ile Glu 155 atg atg tat Met Met Tyr 170 gtg ttg ggc Val Leu Gly 185 gga gca tac Gly Ala Tyr ttt gac acc Phe Asp Thr tgg ctt cgg Trp Leu Arg 235 gtg aat gtc Val Asn Val 250 ttg aag tct Leu Lys Ser 265 gat gtg aaa Asp Val Lys tcc agg qtt Ser Arg Val ctc: tca agg His Asn Ala Gly Phe cgc Arg cat His cac His c ct Pro 140 aag Lys cca Pro aaa Lys gqa Gly acc Th r 220 gca Al a gga Gly ga a Glu cac His ga t Asp 300 aaa cca Pro ga c Asp gtc Val1 tcc Ser ctg Leu 160 atc Ile aca Thr cat His gta Val ggc Gi y 240 ct c Le u ga c Asp t gg T rp atc Ile gat gga Gly tta Leu ccc Pro c ag Gin 145 gt c Val1 aag Lys acc Thr aag Lys ga a Glu 225 caa Gin acq Thr acc Thr gct Ala ga c Asp 305 ca c cag Gin a ca.

Th r gtc Vali 130 atg Met ctc Leu ctg Leu atc Ile aac As n 210 gga Gly gca Ala ctg Leu aac As n cct Pro 290 aac Asn g cc cag Gin gct Ala 115 gcc Ala cgc Arg aac Asn atc: Ile atc Ile 195 ttt Phe cat His gaa Glu tgt Cys act Th r 275 cat His gc t Ala tct tgg T rp 100 gct Al a cat His aca Thr agt Ser acc Thr 180 cca Pro tac Tyr aaa Lys atc Ile tca Se r 260 tca Ser ttg Leu cgc Arg gaa cac His g cc Ala tcc Ser a gc Ser ccg Pro 165 cct Pro ggc Gly ggc Gly a ag L ys cat His 245 aat Asn gac Asp a gc Ser cac His gt a 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 WO 01/00805 PCTIIBOO/00926 Asp Ile Phe Leu Ser Arg Lys Pro Ala Arg Asp His Ala Ser Giu Val 310 315 320 325 ctc aac aac tqg ctg caa tcg aag ctt tcc agc ctc aaa cca tct caa 1123 Leu Asn Asn Trp Leu Gin Ser Lys Leu Ser Ser Leu Lys Pro Ser Gin 330 335 340 taacaccgcg aattatagac tga 1146 <210> 202 <211> 341 <212> PRT <213>. Corynebacterium giutamicum <400> 202 Met Asn Gin Met Gin Gin Trp Lys Pro Asp Phe Leu Giy Glu Giy Tyr 1 5 10 Gin Asn Leu Thr Ile Giu Leu Giy Asp Asp Pro Asp Asn Giu Thr Asp 25 Vai Vai Thr Thr Val Vai Arg Tyr Asn Pro Asp Asn His Aia Asp Giu 40 Ser Phe Ala Aia Arg Pro Ala Leu Leu Trp Val His Giy Met Thr Asp 55 Tyr Phe Phe His Thr Glu Phe Ala Giu Phe Phe His Asn Aia Giy Phe 70 75 Ala Val Tyr Gly Ile Asp Leu Arg Lys Cys Giy Arg Ser Tyr Arg Pro 90 Gly Gin Gin Trp His Tyr Thr Ser Asp Leu Ala His Tyr Phe Pro Asp 100 105 110 Leu Thr Ala Ala Ala*Giu Val Ilie Ser Ser Thr His Pro Glu Leu Val 115 120 125 Pro Val Ala His Ser Thr Gly Gly Leu Ile Val Pro Leu Trp Met Ser 130 135 140 Gin Met Arg Thr Ser Asn Pro Ala Ala Ile Giu Lys Ile Pro Ala Leu 145 150 155 160 Val Leu Asn Ser Pro Trp Leu Asp Met Met Tyr Pro Pro Leu Phe Ile 165 170 175 Lys Leu Ile Thr Pro Met Val Arg Val Leu Gly Lys Arg Ser Pro Thr 180 185 190 Thr Ilie Ile Pro Gly Gly Gly Leu Gly Ala Tyr Gly Lys Ser Ile His 195 200 205 Lys Asn Phe Tyr Gly Giu Trp Asp Phe Asp Thr Thr Ile Lys Pro Val 210 215 220 Giu Gly His Lys Lys Ser Ile G1ly Trp Leu Arg Ala Val Met Ala Giy 225 230 235 240 WO 01/00805 Ala Giu Ile Leu Cys Ser 260 Asn Thr Ser 275 Pro His Leu 290 Asn Ala Arg Ala Ser Giu Lys Pro Ser 340 Asp Se r Val1 Pro 295 Ile Asn His Trp Leu 280 Se r Ph e As n Val1 Leu 265 Asp Ser Leu Trp Asn 250 Lys Val Arg Ser Le u 330 Gly Giu His Asp 300 Lys Ser Asp Th r 270 Gin Glu Al a Leu PCTIBOOIOO926 Le u Asp Trp Ile Asp 320 Ser <210> 203 <211> 630 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(607) <223> RXA01983 <400> 203 atatcgctgc catggcagcc ttgctggqca acctcaagca ccaccgatta ccaggggtgt cctcccatqt ccgctgcgtt acc Thr gqq Gly g ca Ala gCC Al a atg Met ct g Le u cag Gin aat Asn a cc Thr agt Ser cac His cac His at c Ile aa t Asn tcg Ser ccg Pro 99 C Gly acc Thr ctc Leu acg Thr aac As n ca 9 Gin 60 ga c Asp gtg Val1 cactgaccta gaagagctcc atg gaa ggc taC gga Met Giu Gly Tyr Gly 1 aca cag gtc aac aca Thr Gin Val Asn Thr gat ctc ctc ggc gga Asp Leu Leu Gly Gly ctg cag tac gca gtc Leu Gin Tyr Ala Val gca ccg CCC ttc ctg Ala Pro Pro Phe Leu ccg gag caa tcg gct Pro Giu Gin Ser Ala cag tcc acc ctg gta Gin Ser Thr Leu Val 115 163 211 259' 307 355 403 WO 01/00805 PCT/IB00/00926 95 100 ctc att gag ggc ttt ggc cac ggt ttc ctc aat ccc ggg gaa gtc gcg 451 Leu Ile Glu Gly Phe Gly His Gly Phe Leu Asn Pro Gly Glu Val Ala 105 110 115 gag ctg ggg cca aac gtt cga cta gac aat ggt cgg ctc gag cgg gag 499 Glu Leu Gly Pro Asn Val Arg Leu Asp Asn Gly Arg Leu Glu Arg Glu 120 125 130 cct cag aca aat ttc age gcg cag cag agt ccg gga aac ccc ttt gaa 547 Pro Gin Thr Asn Phe Ser Ala Gin Gin Ser Pro Gly Asn Pro Phe Glu 135 140 145 cta cag gga ctt gcc gcc gac cat gag atg atc aag cgc ttt ttc acc 595 Leu Gin Gly Leu Ala Ala Asp His Glu Met Ile Lys Arg Phe Phe Thr 150 155 160 165 ctg cac ctt cgc taagactcta ccttcaccca act 630 Leu His Leu Arg <210> 204 <211> 169 <212> PRT <213> Corynebacterium glutamicum <400> 204 Met Glu Gly Tyr Gly Pro Thr Gin Ile Glu Lys Leu Leu Pro Ala Tyr 1 5 10 Thr Gin Val Asn Thr Ala Gly Asn Asn Pro Ala Thr Thr Pro Glu Gin 25 Asp Leu Leu Gly Gly Ala Ala Thr Ser Pro Glu Asn Tyr Asp His Gin 40 Leu Gin Tyr Ala Val Asp Ala Ser Pro Val His Gin Asn Ala Ala Gin 55 Ala Pro Pro Phe Leu Ile Met His Gly Thr Gly Asp Arg Met Val Pro 70 75 Pro Glu Gin Ser Ala Ala Leu His Thr His Leu Val Gin Ala Gly Arg 90 Gin Ser Thr Leu Val Leu Ile Glu Gly Phe Gly His Gly Phe Leu Asn 100 105 110 Pro Gly Glu Val Ala Glu Leu Gly Pro Asn Val Arg Leu Asp Asn Gly 115 120 125 Arg Leu Glu Arg Glu Pro Gin Thr Asn Phe Ser Ala Gin Gin Ser Pro 130 135 140 Gly Asn Pro Phe Glu Leu Gin Gly Leu Ala Ala Asp His Glu Met Ile 145 150 155 160 Lys Arg Phe Phe Thr Leu His Leu Arg 165 WO 01/00805 WO 0100805PCT/IBOO/00926 <210> 205 <211> 1414 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1414) <223> RXN02947 <400> 205 gtatgttcac acaagaaccc tgcacaacgc cttcaaagta attattcact ctcacccttc aggatttaga ctaagaaacc cgtcgaccac gaccaagcgc atg act gca gca cag 11! Met Thr Ala Ala Gin acc Thr t cc Ser aaa Ly s ttg Leu cgt Arg gt t Val1 tcc Ser gaa Glu ttg Le u gtg Val 150 t Ct Ser cct Pro ctt Leu ca c His ga t Asp aag Lys act Th r gac Asp atc Ile 120 gga Gly ct g Leu gt t Val acc Thr caa Gin a gg Arg ttc Ph e ctg Leu acc Thr ttc Phe atg Met gg9t Gly 140 tca Ser atc Ile acg Thr gct Ala aag Lys gta Val1 ga t Asp atc Ile ggt Gly ga t Asp 125 gct Ala cag Gin tct Ser gct Aia cca Pro act Th r gag Glu gcc Ala gat Asp ggc Gi y 110 ctt Le u ggt Giy atc Ile t tg Leu ctt Leu qta Val1 gag Glu cgt Arg ga c Asp gt g Val 100 tat Tyr gtg Val gg t Gly Ca g Gin gct Al a cg C Arq ga a Glu tat Tyr cac His ggt Gi y ttc Phe ggt Gly cct Pro gt t Val1 gcg Al a 165 ggt Gi y WO 01/00805 WO 0100805PCTIBOOIOO926 gg t Gly ca g Gin acc Th r atg Met 230 gct Al a cgt Arg ca g Gin gat Asp gt c Val1 310 atc Ile g ct Al a gag Glu atc Ile gaa Giu 390 gct Ala cac His act Thr ggt Gly 215 g ct Ala ttg Leu gcg Al a gaa Glu tcc Ser 295 ga c Asp Ct g Le u aac Asn aag Lys ctt Leu 375 ttc Ph e gaa Giu gtg Val1 tcc Ser 200 ga a Giu a cc Thr gat Asp ga a Glu aac Asn 280 C cg Pro ga c Asp tgt C ys cag Gin gct Ala 360 gag Glu gac Asp gca Al a tac tcc Tyr Ser 185 aag atg Lys Met gat gtc Asp Val tcc ggt Ser Gly tgg gtt Trp Val 250 act cct Thr Pro 265 atc aac Ile Asn aac cag Asn Gin gcc gag Ala Glu ggc ttc Gly Phe 330 cca acc Pro Thr 345 gcc cgt Ala Arg ttc gtg Phe Val ggc atc Gly Ile acc gtc Thr Val 410 cc t Pro ttt Phe acc Th r acc Th r 235 cgc Arg cgc Arg ga t Asp cct Pro ttc Phe 315 gct Ala cag Gin ttc Phe gac Asp atc Ile 395 ggc Gly gct Al a atc Ile ca g Gin 220 tcc Ser gag Giu ca g Gin gtg Val1 tat Tyr 300 ttc Ph e cg c Arg ttc Phe atc Ile gtt Val 380 cgc Arg aag Ly s ctg Leu a cc Thr 205 gag Giu cat His ct g Leu gag Giu ga t Asp 285 ga c Asp gag Glu gtt Val gct Ala cg c Arg 365 cca Pro cg c Arg atc Ile act Th r 190 ggc Gly gag Giu tac Tyr acc Thr gcc Ala 270 ctg Leu atg Met atc Ile gag Gi u ggc Gly 350 acc Th r ggc Gly 99C Gly acc Thr ga c Asp cct Pro ctc Leu tct.

Ser tct Ser 255 gac Asp gaa Giu aa g Lys cag Gin gtc Val 335 tgc Cys tgc Cys ttc Phe gca Ala gtc Val1 415 ttc Phe gat Asp gg t Gly gct Ala 240 tat Tyr atc Ile ttg Leu ga a Giu gaa Glu 320 cgt Arg ttg Leu gat Asp ctg Leu aag Lys 400 atc Ile atc Ile gtc Val g gc Gly 225 t ct.

Ser ctt Le u a tg Met ga c Asp gtt Val1 305 ga c Asp tct Ser gat Asp gcc Ala cct Pro 385 ct g Le u acc Thr gtc Val1 atc Ile 210 gct Al a gat Asp cca Pro atc Ile acc Thr 290 att Ile tac Tyr gtt Val1 att Ile ttc Phe 370 ggc Gly ctt Leu cgc Arg atg Met 195 aag Lys cac His ga t Asp t cc Se r ggt Gly 275 atc Ile tcc Ser g ca Ala ggc Gly aag Lys 355 aac As n acc Th r tac Tyr aag Lys gtg Val1 act Thr acc Thr t ca Ser aac Asn 260 tcc Ser atc Ile cgc Arg gag Gi u atc Ile 340 gca Ala atc Ile aac Asn gct Ala t cc Ser 420 ga t Asp gtc Val1 ca c His gat Asp 245 aac Asn atc Ile ccg Pro atc Ile aac As n 325 gtg Val1 tct Ser cca Pro ca g Gin tac T yr 405 tac Tyr 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 WO 01/00805 PCT/IBOO/00926 ggc gga gcg tac tgc gtg atg qgt tcc aag gat atg ggc gct ggc ctg 1411 Gly Gly Ala Tyr Cys Val Met Gly Ser Lys Asp Met Gly Ala Gly Leu 425 430 435 gta 1414 Val1 <210> 206 <211> 438 <212> PRT <213> Corynebacterium glutamicum <400> 206 Met Thr Ala Ala Gin Thr Lys Pro Asp Leu Thr Thr Thr Ala Gly Lys 1 5 10 Leu Ser Asp Leu Arg Ser Arg Leu Ala Giu Ala Gin Ala Pro Met Gly 25 Glu Ala Thr Val Glu Lys Val His Ala Ala Gly Arg Lys Thr Ala Arg 40 Glu Arg Ile Giu Tyr Leu Leu Asp Glu Gly Ser Phe Val Glu Ile Asp 55 Ala Leu Ala Arg His Arg Ser Lys Asn Phe Gly Leu Asp Ala Lys Arg 70 75 Pro Val Thr Asp Gly Val Val Thr Gly Tyr Gly Thr Ile Asp Gly Arg 90 Lys Val Cys Val Phe Ser Gin Asp Gly Ala Val Phe Gly Gly Ala Leu 100 105 110 Gly Glu Val Tyr Gly Glu Lys Ile Val Lys Val Met Asp Leu Ala Ile 115 120 125 Lys Thr Gly Val Pro Leu Ile Gly Ile Asn Glu Gly Ala Gly Ala Arg 130 135 140 Ile Gin Giu Gly Val Val Ser Leu Gly Leu Tyr Ser Gin Ile Phe Tyr 145 150 155 160 Arg Asn Thr Gin Ala Ser Gly Val Ile Pro Gin Ile Ser Leu Ile Met 165 170 175 Gly Ala Cys Ala Gly Gly His Val Tyr Ser Pro Ala Leu Thr Asp Phe 180 185 190 Ile Val Met Val Asp Gin Thr Ser Lys Met Phe Ile Thr Gly Pro Asp 195 200 205 Val Ile Lys Thr Val Thr Gly Giu Asp Val Thr Gin Glu Giu Leu Gly 210 215 220 Gly Ala His Thr His Met Ala Thr Ser Gly Thr Ser His Tyr Ser Ala 225 230 235 240 Sex: Le u Met Asp Val 305 Asp Se r Asp Ala Pro 385 Leu Thr Met WO 01/00805 Asp Asp S Pro Ser A~ Ile Gly SE 275 Thr Ile I] 290 Ile Ser Ax Tyr Ala Gl Val Gly I] 34 Ile Lys Al 355 Phe Asn Il 370 Gly Thr As Leu Tyr Al Arg Lys Se 42 Gly Ala Gl 435 e0 sU 60 '0 a Asp 245 Asn Ile Pro Ile As n 325 Val1 Ser Pro Gin T yr 405 Tyr Leu Ala Arg Gin Asp Val 310 Ile Ala Giu Ile Glu 390 Ala Gly Val1 Le u Ala Giu Ser 295 Asp Leu Asn L ys Leu 375 Phe Giu Giy Asp Glu Asn 280 Pro Asp Cys Gin Ala 360 Glu Asp Al a Al a T rp Th r 265 Ile As n Al a Gly Pro 345 Al a Phe Gi y Th r Tyr 425 Val1 250 Pro Asn Gin Glu Phe 330 Thr Ar g Val1 Ile Val1 410 Cys Arg Arg Asp Pro Phe 315 Al a Gin Phe Asp Ile 395 Gi y ValI Glu Gin Val1 T yr 300 Phe Arg Phe Ile ValI 380 Arg Lys Met Le u Glu Asp 285 Asp Giu Val1 Ala Arg 365 Pro Arq Ile Gly Th r Ala 270 Leu Met Ile Gi u Gi y 350 Th r Gly Gly Th r Ser 430 Se r 255 Asp Giu Lys Gin Val 335 Cys Cys Phe Al a Val1 415 Lys PCTIIBOO/00926 Tyr Ile Leu Glu Glu 320 Arg Leu Asp Leu Lys 400 Ile Asp (210> 207 <211> 688 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(688) <223> FRXA02320 <400> 207 gtatgttcac acaaqaaccc tgcacaacgc cttcaaagta cgtcgaccac gaccaagcgc attattcact ctcacccttc aggatttaga ctaagaaacc atg act gca gca cag Met Thr Ala Ala Gin 1 acc aaa cct gac ctc acc acc acg gct gga aag ctg tcc gat ctt cgc Thr Lys Pro Asp Leu Thr Thr Thr Ala Giy Lys Leu Ser Asp Leu Arg 15 WO 01/00805 tcc cgt ctt gca Ser Arg Leu Ala aaa gtg cac gct Lys Val His Ala ttg ctc gat gag Leu Leu Asp Giu cgt toc aag aac Arg Ser Lys Asn gtt qtq act ggt Val Val Thr Gly tcc cag gac ggc Ser Gin Asp Gly 105 gaa aaq atc gtt Glu Lys Ile Val 120 ttg atc gga atc Leu Ile Gly Ile 135 gtg tot ctq ggt Vai Ser Leu Gly 150 tot ggc gtt atc Ser Gly Val Ile caa Gin agg Arg ttc Ph e ct g Leu acc Thr ttc Phe atq Met ggt Gly 140 tca Ser atc Ile gct Ala aag L ys gt a Val1 ga t Asp atc Ile ggt Gi y ga t Asp 125 gc t Aia cag Gin tct Se r cca Pro act Thr gag Giu gco Ala gat Asp ggo Gly 110 ctt Leu ggt Giy atc Ile ttg Le u a tg Met gcc Ala atc Ile aag Ly s ggc Gly g ct Ala gcg Ala g cg Al a ttc Phe atc Ile 175 gg c Gly cgc Arg ga t Asp cgt Arg 80 cgt Arg ttg Le u atc Ile cgt Arg tac Tyr 160 atg Met PCTIIBOO/00926 gaa 211 Glu tat 259 T yr cac 307 His ggt 355 Gi y ttc 403 Phe ggt 451 Gly cct 499 Pro gtt 547 Val1 gog 595 Al a 165 ggt 643 Gly 688 ggt cac gtg tao toc Oct gct ctg act gac ttc atc gtc atg gtg Gly His Val Tyr 185 Ser Pro Ala Leu Thr Asp Phe Ile Val Met Val <210> 208 <211> 196 <212> PRT <213> Corynebacterium glutamicun <400> 208 Met Thr Ala Ala Gin Thr Lys Pro 1 5 Leu Ser Asp Leu Arg Ser Arg Leu Giu Ala Thr Val Giu Lys Val His 40 Giu Arg Ile Glu Tyr Leu Leu Asp Asp Leu Thr Thr Thr Ala Gly Lys 10 Ala Giu Ala Gin Ala Pro Met Gly 25 Ala Aia Gly Arg Lys Thr Ala Arg Glu Gly Ser Phe Val Glu Ile Asp WO 01/00805 PCTJIBOO/00926 55 Ala Leu Ala Arg His Arg Ser Lys Asn Phe Gly Leu Asp Ala Lys Arg 70 75 Pro Val Thr Asp Gly Val Val Thr Gly Tyr Gly Thr Ile Asp Gly Arg 90 Lys Val Cys Val Phe Ser Gin Asp Gly Ala Val Phe Gly Gly Ala Leu 100 105 110 Gly Giu Val Tyr Gly Giu Lys Ile Val Lys Val Met Asp Leu Ala le 115 120 125 Lys Thr Gly Val Pro Leu Ile Gly Ile Asn Giu Gly Ala Gly Ala Arg 130 135 140 Ile Gin Giu Gly Val Val Ser Leu Gly Leu Tyr Ser Gin Ile Phe Tyr 145 150 155 160 Arg Asn Thr Gin Ala Ser Gly Val Ile Pro Gin Ile Ser Leu Ile Met 165 170 175 Gly Ala Cys Ala Gly Gly His Val Tyr Ser Pro Ala Leu Thr Asp Phe 180 185 190 Ile Val Met Val 195 <210> 209 <211> 519 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (519) <223> <400> 209 cct cgc cag aag gcc gac atc atg atc ggt tcc atc cag gaa aac atc 48 Pro Arg Gin Lys Aia Asp Ile Met Ile Giy Ser Ile Gin Giu Asn Ile 1 5 10 aac gat gtg gat ctg gaa ttg gac acc atc atc ccg gat tcc ccg aac 96 Asn Asp Val Asp Leu Glu Leu Asp Thr Ile Ile Pro Asp Ser Pro Asn 25 cag cct tat gac atg aag gaa gtt att tcc cgc atc gtn gac gac qcc 144 Gin Pro Tyr Asp Met Lys Giu Val Ilie Ser Arg Ile Val Asp Asp Ala 40 gag ttc ttc gag atc cag gaa gac tac gca gag aac atc ctg tgt ggc 192 Glu Phe Phe Giu Ile Gin Giu Asp Tyr Ala Giu Asn Ile Leu Cys Giy 55 ttc gct cgc gtt gag gnc cgt tnt gtt ggc atc gtg gct aac cag cca 240 Phe Ala Arg Val Giu Xaa Arg Xaa Val Giy Ilie Vai Ala Asn Gin Pro 70 75 WO 01/00805 PCT/EBOO/00926 acc cag ttc gct ggc tgn ttg gat att aag gca tct gag aag gct gcc 288 Thr Gin Phe Ala Gly Xaa Leu Asp Ile Lys Ala Ser Giu Lys Ala Ala 90 cgt ttc atc cgc acc tgc gat gcc ttc aac atc cca atc ctt gag ttc 336 Arg Phe Ile Arg Thr Cys Asp Ala Phe Asn Ile Pro Ile Leu Giu Phe 100 105 110 gtg gac gtt cca ggc ttc ctg cct ggc acc aac cag gaa ttc gac qgc 384 Val Asp Val Pro Gly Phe Leu Pro Gly Thr Asn Gin Giu Phe Asp Gly 115 120 125 atc atc cgc cgc ggc gca aag ctg ctt tac gct tac gct gaa gca acc 432 Ile Ile Arg Arg Gly Ala Lys Leu Leu Tyr Ala Tyr Ala Giu Ala Thr 130 135 140 gtc ggc aag atc acc gtc aic acc cgc aag tcc tac ggc gga gcg tac 480 Val Gly Lys Ile Thr Val Ile Thr Arg Lys Ser Tyr Giy Gly Ala Tyr 145 150 155 160 tgc gtg atg ggt tcc aag gat atg ggc gct ggc ctg gta 519 Cys Val Met Gly Ser Lys Asp Met Gly Ala Gly Leu Vai 165 170 <210> 210 <211> 173 <212> PRT <213> Corynebacterium giutamicum <400> 210 Pro Arg Gin Lys Ala Asp Ile Met Ile Gly Ser Ile Gin Giu Asn Ile 1 5 10 Asn Asp Vai Asp Leu Giu Leu Asp Thr Ile Ile Pro Asp Ser Pro Asn 25 Gin Pro Tyr Asp Met Lys Glu Val Ile Ser Arg Ile Val Asp Asp Ala 40 Giu Phe Phe Giu Ile Gin Giu Asp Tyr Aia Giu Asn Ile Leu Cys Gly 55 Phe Ala Arg Val Giu Xaa Arg Xaa Vai Gly Ile Val Ala Asn Gin Pro 70 75 Thr Gin Phe Ala Giy Xaa Leu Asp Ile Lys Ala Ser Giu Lys Ala Ala 90 Arg Phe Ile Arg Thr Cys Asp Ala Phe Asn Ile Pro Ile Leu Giu Phe 100 105 110 Val Asp Val Pro Giy Phe Leu Pro Gly Thr Asn Gin Giu Phe Asp Gly 115 120 125 Ile Ile Arg Arg Gly Ala Lys Leu Leu Tyr Ala Tyr Ala Glu Ala Thr 130 135 140 Val Gly Lys Ile Thr Val Ile Thr Arg Lys Ser Tyr Gly Gly Ala Tyr 145 150 155 160 WO 01/00805 WO 0100805PCT/IBOO/00926 Cys Val Met Gly Ser Lys Asp Met Gly Ala Gly Leu Val 165 170 <210> <211> <212> <213> <220> <221> <222> <223> 211 1752

DNA

Corynebacteriun

CDS

(101)..(1729) RXN0 2321 glut am icum <400> 211 tttaaaaact acccgcacgc agcacgaacc tgttcagtga tgtaaatcac cgcggaaata ttgtggacgt tacccccgcc taccgctacg atttcaaaac atg acc att tcc tca Met Thr Ile Ser Ser cct ttg att gac Pro Leu Ile Asp gcc aac ctt cca gac atc aac acc act Ala Asn Leu Pro Asp Ile Asn Thr Thr gcc ggc Ala Gly aag atc gcc Lys Ile Ala gqt qaa aag Gly Giu Lys gac Asp ctt aag gct cgc Leu Lys Ala Arg gcg gaa gcc cat Ala Glu Ala His ttc ccc atg Phe Pro Met ctc act gcc Leu Thr Ala gca gta qag aag gtc cac gct gct gga Ala Val Glu Lys Val His Ala Ala Gly cgc Arg.

cgt gag Arg Giu cgc ttg gat tac Arg Leu Asp Tyr tta Leu ctc gat gag ggc Leu Asp Glu Gly ttc atc gag acc Phe Ile Glu Thr cag ctg gct cgc Gin Leu Ala Arg cgc acc acc gct Arg Thr Thr Ala tgc ctg ggc gct Cys Leu Giy Ala cgt cct gca acc Arg Pro Ala Thr ggt atc gtg acc Gly Ilie Val Thr tgg ggc acc att Trp Gly Thr Ile gat gga Asp Giy 100 cgc gaa gtc Arg Glu Val ctt ggt gag Leu Gly Giu 120 atc ttc tcg cag Ile Phe Ser Gin ggc acc gta ttc Gly Thr Val Phe ggt ggc gcg Gly Gly Ala 115 gag ctg gca Glu Leu Ala 451 499 gtg tac ggc gaa Val Tyr Gly Giu atg atc aag atc Met Ile Lys Ile atc gac Ile Asp 135 acc ggc cgc cca Thr Gly Arg Pro atc ggt ctt tac Ile Gly Leu Tyr ggc gct ggc gct Giy Ala Gly Ala cg t Arg 150 att cag gac ggc Ilie Gin Asp Gly gct Al a 155 gtc tcc ctg gac Val Ser Leu Asp att tcc cag acc Ile Ser Gin Thr WO 01/00805 WO 0100805PCTIIBOO/00926 ra c Tyr atg Met ttc Ph e ga c Asp gg C Gly 230 gc t Ala ttc Phe gag Giu ctc Leu ga t Asp 310 gca Ala cag Gin ct g Le u ga c Asp ctc Leu 390 aa g caa Gin ggc Giy gt g Val1 gtg Val 215 gga Gly gcg Ala ctc Le u ga a Gi u ga c Asp 295 gt c Val1 ga c Asp tcc Ser gac Asp gcg Ala 375 cca Pro ctg aac att cag qct Asl Ile Gin Ala 170 gca tgt gca ggt Ala Cys Ala Gly 185 gtc atg gtg gac.

Val Met Val Asp 200 atc aag acc gtc Ile Lys Thr Val gca dcc acc cac Ala Thr Thr His 235 acc gat gag gaa Thr Asp Giu Giu 250 cca tcc .aac aat Pro Ser Asn Asn 265 gaa ggc ggc gtt Glu Gly Gly Vai 280 gag atc atc cca Gu Ile Ile Pro atc gaa tgc ctc Ile Giu Cys Leu 315 cgc gca gaa aac Arg Ala Glu Asn 330 gtt ggc ttt gtt Val Gly Phe Val 345 atc gac tcc tct Ile Asp Ser Ser 360 ttc aac atc cca Phe Asn Ile Pro ggc gca ggc cag Giy Ala Gly Gin 395 ctc tac gca tac tct ggc qtt atc cca cag atc tcc Ser Gly Val Ile Pro Gin Ile Ser 175 ggc Gly aag Lys acc Thr 220 atg Met gca Al a cgc Arg gaa Giu gat Asp 300 acc Th r gtt Val1 gcc Ala gag Glu atc Ile 380 gag Glu 99C gc t Ala 190 tcc Ser gag, Glu acc Th r gat Asp tac Tyr 270 aac As n gcg Ala ga t Asp att Ile ca g Gin 350 g ca Ala atg Met ggt Gly g ca tac Tyr aag Lys gaa Giu gct Ala tgg Trp 255 gca Al a atc Ile acc Th r gg c Gi y gca Ala 335 cca Pro gct Ala ctt Leu ggc Gly ggc Gly atg Met a tc Ile gg t Gly 240 gta Val cc g Pro acc Thr gtt Val gaa Giu 320 ttc Phe acc Thr cgc Arg g tc Val1 att Ile 400 cca gct Pro Ala ttc gtt Phe Val 210 acc cag Thr Gin 225 aac tcc Asn Ser cag gac Gin Asp atg gaa Met Glu gct gac Ala Asp 290 cct tac Pro Tyr 305 tac ctg Tyr Leu ggc cgc Gly Arg cag ttc Gin Phe ttc gtc Phe Val 370 gac gtc Asp Val 385 ctg cgt Leu Arg ctg Leu 195 acc Thr gaa Giu ca c His ctg Le u ga c Asp 275 qa t Asp ga c Asp ga a Giu atc Ile gc t Ala 355 cgc Arg ccc Pro cg t Arg gt c ValI 180 a cc Th r Giy gag Glu tac T yr g tg Vali 260 ttc Phe ctg Le u gt c Val1 atc Ile gaa Giu 340 9gc Gly acc Th r ggc Gly ggc Gi y atc Ile ga c Asp cca Pro ctt Leu acc Thr 245 t cc Se r gac Asp aag Lys cgc Arg cag Gin 325 ggc Gly t gc Cys t gc Cys ttc Phe gca Al a 405 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 acc gtt cca aag atc acc gtc wo 01/00805 WO 0100805PCT/IBOO/00926 Lys Leu Leu Tyr atg Met ttg Leu atg Met 455 gca Ala gag Glu cgt Arg att Ile cgt Arg ggc Gly 440 ggc Gly gct Ala cgc Arg ggc Gly tc Ser 520 aag Lys 425 tct Ser gct Ala ga t Asp gag Glu ct g Leu 505 cgc Arg Ala 410 gct Ala gac Asp gct Ala gcc Al a tat T yr 490 atc Ile aac As n T yr ta c Tyr atc Ile gg C Gi y aag Lys 475 ga a Glu ga c Asp ct t Leu Ala g cg Ala 4 30 g ca Al a gga Gly ga t Asp atg Met atc Ile 510 ct C Leu Thr 415 tac Tyr t gg Trp ttc Phe a cc Thr ctc Leu 495 ctg Leu aag Lys Val Pro Lys tgc gtg atg Cys Val Met cca acc gca Pro Thr Ala 450 atc tac cgc Ile Tyr Arg 465 gta gct ctg Val Ala Leu 480 aac ccg tac Asn Pro Tyr cca agc gaa Pro Ser Glu cac aag aac His Lys Asn I le gg t Gi y 435 ca g Gln aag Lys g ct Ala cac His a cc Thr 515 gtc Val1 Thr 420 t cc Ser atc Ile gag Glu aag Lys gct Al a 500 cgc Arg act Thr ValI aag Lys gcc Ala ct c Leu t cc Ser 485 gca Ala gga Gl y cqc Arg 1411 1459 1507 1555 1603 1651 1699 1749 1752 cct gCt cgc aag cac ggc aac atg cca ctq Pro Ala Arg Lys His Gly Asn Met Pro Leu 535 540 ggt <210> 212 <211> 543 <212> PRT <213> Corynebacterium glutamicum <400> 212 Met Thr Ile Ser Ser Pro Leu Ile Asp Val 1 5 10 Asn Thr Thr Ala Gly Lys Ile Ala Asp Leu Ala His Phe Pro Met Gly Glu Lys Ala Val Gly Arg Leu Thr Ala Arg Glu Arq Leu Asp Ser Phe Ile Glu Thr Asp Gln Leu Ala Arg 70 Cys Leu Gly Ala Lys Arg Pro Ala Thr Asp 90 taaatcggcg aatccataaa Asp Al a Ala Glu Ala Gly Ile Glu Ala Gly Phe T rp wo 01/00805 WO 0100805PCT[[BOO/00926 Gi y Val1 Ile Giu 145 Ile Gin Pro Phe Th r 225 As n Gin Met Al a Pro 305 Tyr Gi y Gin Phe Asp 385 Leu Ile Gly 115 Giu Aia Gin Ser Le u 195 Thr Giu His Le u Asp 275 Asp Asp Giu Ile Ala 355 Arg Pro Arg Asp 100 Giy Leu Gi y Th r Vali 180 Thr Giy Giu Tyr Val1 260 Phe Le u Vali Ile Glu 340 Gly Thr Gly Gly Gi y Al a Ala Ala Phe 165 Ile Asp Pro Leu Thr 245 Ser Asp Lys Arg Gin 325 Gly Cys Cys Phe Al a 405 Ar g Le u Ile Arg 150 Tyr Met Phe Asp Gly 230 Ala Phe Giu Leu Asp 310 Al a Gin Leu Asp Leu 390 Lys Giu Gly Asp 135 Ile Gin Gi y Val1 Val1 215 Giy Al a Leu Glu Asp 295 Val1 Asp Ser Asp Ala 375 Pro Leu Cys 105 Val1 Giy Asp Ile Cys 185 Met Lys Thr Asp Se r 265 Gi y Ile Giu Al a Gly 345 Asp As n Ala Tyr Phe Gly Pro Ala 155 Ala Gly Asp Val1 His 235 Giu Asn Val1 Pro Leu 315 Asn Val1 Ser Pro Gin 395 T yr Se r Glu Leu 140 Val1 Se r Gi y Lys Th r 220 Met Ala Arg Giu Asp 300 Thr Val1 Al a Giu Ile 380 Giu Gly Gin Lys 125 Ile Se r Gly Asn Thr 205 Gly Val Le u Se r Giu 285 Se r Asp Val As n Lys 365 Val1 Tyr Glu WO 01/00805 Pro Lys Ilie Thr Val Thr Met Arg Lys 420 425 Val Met Gly Ser Lys Gly Leu Gly Ser 435 440 Thr Ala Gin Ilie Ala Val Met Gly Ala 450 455 Tyr Arg Lys Giu Leu Met Ala Ala Asp 465 470 Ala Leu Ala Lys Ser Phe Glu Arg Glu 485 Pro Tyr His Ala Ala Glu Arg Gly Leu 500 505 Ser Glu Thr Arg Gly Gin Ile Ser Arg 515 520 Lys Asn Val Thr Arg Pro Ala Arg Lys 530 535 <210> 213 <211> 467 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (444) <223> FRXA02321 <400> 213 gag tac ggt ggc att ctg cgt cgt ggc Glu Tyr Gly Gly Ilie Leu Arg Arg Gly 1 5 gnn gaa gca ccg gtt cca aag atc acc Xaa Glu Ala Pro Val Pro Lys Ile Thr 25 ggc gga gcg tac tgc gtg atg ggt tcc Gly Gly Ala Tyr Cys Val Met Gly Ser 40 aac ctt gca tgg cca acc gca cag atc Asn Leu Ala Trp Pro Thr Ala Gin Ilie 55 gca gtt gga ttc atc tac cgc aag gag Ala Val Gly Phe Ilie Tyr Arg Lys Glu 70 ggc ctc gat acc gta gct ctg gct aag Gly Leu Asp Thr Val Ala Leu Ala Lys gac cac atg ctc aac ccg tac cac gct Ala Asp Ala Ala Tyr 490 Ile Asn His gca Ala 10 gtc Val aag Lys gcc Ala ct C Le u tcc Ser 90 gca Tyr Ile Gly Lys5 475 Glu Asp Leu Gb y aag Lys acc Thr gg C Gly gt c Val atg Met ttC Phe gaa Gly As n Al a 4 Gly Asp Al a Arg As n 540 ctg Le u atg Met ttg Le u atg Met gca Ala gag Glu cg t Gly Leu 445 Val1 Leu His Val1 Leu 525 Met ctc Leu cgt Arg ggC Gly ggc Gly gct Ala cgc Arg 99C Ala 430 Al a Gly Asp Met Ile 510 Leu Pro tac Tyr aag Lys tct Ser gct Ala ga t Asp gag Glu ctg Tyr Trp Phe Thr Le u 495 Le u Lys Leu g ca Ala gct Ala ga c Asp gc t Ala gcc Ala tat Tyr atc PCTIIBOO/00926 Cys Pro Ile Val 480 Asn Pro His 48 96 144 192 240 288 336 WO 01/008(05 Asp His Met Leu Asn Pro Tyr His Ala 100 105 gcc qtg atc ctg cca aqc gaa acc cgc Ala Val Ile Leu Pro Ser Giu Thr Arg 115 120 cgc ctg ctc aag cac aag aac gtc act Arq Leu Leu Lys His Lys Asn Val Thr 130 135 aac atg cca ctq taaatcggcq aatccata Asn Met Pro Leu 145 <210> 214 <211> 148 <212> PRT <213> Corynebacterium giutamicum <400> 214 Glu Tyr Gly Gly Ile Leu Arg Arg Gly 1 5 Xaa Glu Ala Pro Vai Pro Lys Ilie Thr Gly Gly Ala Tyr Cys Val Met Gly Ser 40 Asn Leu Ala Trp Pro Thr Ala Gin Ile 55.

Ala Val Gly Phe Ilie Tyr Arq Lys Giu 70 Gly Leu Asp Thr Val Ala Leu Ala Lys Asp His Met Leu Asn Pro Tyr His Ala 100 105 Ala Val Ile Leu Pro Ser Glu Thr Arq 115 120 Arg Leu Leu Lys His Lys Asn Val Thr 130 135 Asn Met Pro Leu 145 <210> 215 <211> 514 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(514) PCTJIBOO/00926 Ala Glu Arg Gly Leu Ile Asp 110 gga cag att tcc cgc aac ctt 384 Gly Gin Ile Ser Arg Asn Leu 125 cqc cct gct cgc aaq cac ggc 432 Arg Pro Ala Arg Lys His Gly 140 aa ggt 467 Ala Val1 Lys Ala Le u S er Ala Gly Arg Ly s Thr GI y Val1 Met Ph e Gi u Gin Pro Leu Met Leu Met Ala Glu Arg Ile Ala 140 Leu Arg Gly Giy Ala Arg Gly Ser 125 Arg WO 01/00805 PCT/IBOO/00926 <223> FRXA02343 <400> 215 tttaaaaact acccgcacgc aqcacqaacc tgttcagtga tgtaaatcac cgcqgaaata ttgtggacgt tacccccgcc taccgctacg atttcaaaac atg acc att tcc tca 115 Met Thr Ile Ser Ser 1 cct ttg att gac gtc 9cc aac ctt cca gac atc aac acc act qcc ggc 163 Pro Leu Ile Asp Vai Ala Asn Leu Pro Asp Ile Asn Thr Thr Ala Gly 15 aag atc gcc gac ctt aag gct cgc cgc gcg gaa gcc cat ttc ccc atg 211 Lys Ile Ala Asp Leu Lys Ala Arg Arg Ala Glu Ala His Phe Pro Met 30 ggt gaa aag gca gta gag aag gtc cac gct gct gga cgc ctc act qcc 259 Gly Giu Lys Ala Val Giu Lys Val His Ala Ala Gly Arg Leu Thr Ala 45 cgt gag cgc ttg gat tac tta ctc gat gag ggc tcc ttc atc gag acc 307 Arg Glu Arg Leu Asp Tyr Leu Leu Asp Giu Giy Ser Phe Ile Giu Thr 60 gat cag ctq gct cqc cac cgc acc acc gct ttc tgc ctq ggc gct aag 355 Asp Gin Leu Ala Arg His Arg Thr Thr Ala Phe Cys Leu Gly Ala Lys 75 80 cqt cct gca acc gac ggc atc gtg acc ggc tqg ggc acc att gat qga 403 Arg Pro Ala Thr Asp Gly Ile Val Thr Gly Trp Gly Thr Ile Asp Gly 95 100 cgc gaa gtc tgc atc ttc tcg cag gac ggc acc gta ttc ggt ggc gcg 451 Arg Giu Val Cys Ile Phe Ser Gln Asp Gly Thr Val Phe Gly Gly Ala 105 110 115 ctt ggt gag gtg tac ggc gaa aag atg atc aag atc atg gag ctg gca 499 Leu Gly Glu Val Tyr Gly Glu Lys Met Ile Lys Ile Met Glu Leu Ala 120 125 130 atc gac acc ggc cgc 514 Ile Asp Thr Giy Arg 135 <210> 216 <211> 138 <212> PRT <213> Corynebacterium glutamicum <400> 216 Met Thr Ile Ser Ser Pro Leu Ile Asp Vai Ala Asn Leu Pro Asp Ile 1 5 10 Asn Thr Thr Ala Gly Lys Ile Ala Asp Leu Lys Ala Arg Arg Ala Glu 25 Ala His Phe Pro Met Gly Giu Lys Ala Val Giu Lys Val His Ala Ala 40 WO 01/00805 Gly Arg .Leu Thr Ala Arg Giu Arg Leu 55 Ser Phe Ile Giu Thr Asp Gin Leu Ala 70 Cys Leu Gly Ala Lys Arg Pro Ala Thr Gly Thr Ile Asp Gly Arg Giu Vai Cys 100 105 Val Phe Gly Giy Ala Leu Gly Giu Val 115 120 Ile Met Giu Leu Ala Ile Asp Thr Gly 130 135 <210> 217 <211> 492 <212> DNA <213> Corynebacterium glutamicum Asp Tyr Leu Arg His Arg 75 Asp Gly Ile 90 Ile Phe Ser Tyr Gly Glu Arg Leu Asp Glu Thr Thr Ala Vai Thr Gly Gin Asp Gly 110 Lys Met Ile 125 PCTIBOO/00926 Gly Ph e Trp Thr Lys <220> <221> CDS <222> <223> FRXA02850 <400> 217 gaa Glu 1 cac His ctg Leu ga c Asp gat Asp ga c Asp ga a Giu atc Ile gag Giu tac Tyr gtg Val1 ttc Phe ctq Leu gt c Val1 atc Ile gaa Glu ctt Leu acc Thr t cc Ser gac Asp aag Lys cg c Arg cag Gin Gly 115 9gc Gi y gct Al a ttc Phe gag Giu ct C Leu gat Asp gca Ala 100 cag Gin gga Gly gcg Ala ctc Leu gaa Giu ga C Asp qtc Val1 gnc Xaa tcc Se r acc Thr ga t Asp tac Tyr aac As n gcg Ala gat Asp att Ile cag Gin g ct Ala t gg Trp g ca Ala atc Ile acc Thr ggc Gly gca Al a cca Pro 125 ggt Giy gta Val C cg Pro a cc Thr gtt Val g aa Giu ttc Phe 110 acc Thr WO 01/00805 PCTIIBOO/00926 gct ggc tgc ctg gac atc qac tcc tct gag aag gca gct cgc ttc gtc 432 Ala Gly Cys Leu Asp Ile Asp Ser Ser Glu Lys Ala Ala Arg Phe Val 130 135 140 cgc acc tgc qac gcg ttc aac atc cca atc gtc atg ctt gtg qac gtc 480 Arq Thr Cys Asp Ala Phe Asn Ile Pro Ile Val Met Leu Val Asp Val 145 150 155 160 ccc ggc ttc ctc 492 Pro Gly Phe Leu <210> 218 <211> 164 <212> PRT <213> Corynebacterium glutarnicum <400> 218 Glu Glu Leu Gly Gly Ala Thr Thr His

I

His Le u Asp Asp Asp Glu Ile Ala Arq 145 Pro Tyr Val1 Phe Le u Val1 Ile Glu Gly 130 Thr Gly 5 Ala Leu Glu Asp Val1 Xa a Se r Asp Al a Th r Pro Glu Glu 70 Xaa Arg Val1 Ile Phe 150 Asp Se r Gi y 55 Ile Giu Ala Gly Asp 135 As n Glu As n Gly Ile Cys GI u Phe 120 Ser Ile Met Ala Ar q Glu Asp Thr Val1 Al a Glu Ile Th r Asp T yr Asn Ala Asp Ile Gin Ala 140 Met Ala Trp Al a Ile Thr Giy Al a Pro 125 Al a Le u Se r Asp Glu Asp Tyr Leu Arg Phe Val1 Vai 160 <210> 219 <211> 1671 <212> DNA <213> Corynebacteriun qiutarnicum <220> <221> CDS WO 01/00805 <222> (1648) <223> RXA02583 <400> 219 PCTIBOO/00926 cagttgtcga tgaaccagaa atcggcacag tcggagctca tttgagtcgc cgcattgatg agatttctcg gaagaattag taacggagag ctgacggaag ttg agt aac acc act Leu Ser Asn Thr Thr act Thr gac Asp acc Th r gtg Val1 tac Tyr gtg Val1 at g Met att Ile caq Gin cag Gin 150 aaa Lys atc Ile gca Ala c ca Pro acc Thr gag Glu tct Ser gcc Ala act Thr ggt Gly gat Asp 135 ctt Le u tcq Se r gg c Gi y gag aag Glu Lys ggt. agt Gly Ser cct cgt Pro Arg atc gqc Ile Gly gac ggt Asp Gly atc tac Ile Tyr ttc gga Phe Gly 105 tgc cca Cys Pro 120 gcg gtg Ala Vai ccq ctg Pro Leu qca ggt Ala Gly gtt gat Val Asp 185 ct a Le u ga a Giu cag Gin g ca Ala gtg Val1 gcc Ala cgt Arg gt t Val acc Thr tct Se r ggc Gly 170 gg t Gi y gcg Al a cgc Arg cg t Arg eta Le u gtg Val1 75 cat His aaa Lys atc Ile t cc Ser ggc Gly 155 g ca Ala gaa Glu ga t Asp g ca Al a att Ile Giy 60 act Th r gac Asp gtc Val ggt Gly ttg Le u 140 cg c Arg gtg Val1 aca Thr gag Glu ctg Le u cgt Arg ga t Asp 45 cgt Arg ggt Gly aag Lys agc Ser att Ile 125 gcg Al a agc Ser tat Tyr gaa Glu cgc Arg aaa Lys gct Al a acc Thr tat Tyr acc Th r gaa Giu 110 ca g Gin atg Met cct Pro gca Ala atg Met 190 g ca Al a 15 aag Ly s ct g Leu cg Pro ggt Gly gtt Val1 95 gte Val1 ga t Asp tac T yr ca g Gin cct Pro 175 tat Tyr cge A rg cgc Arg ctt Leu ga t Asp cg e Arg 80 tac Tyr atg Met tcc Ser t ca Ser att Ile 160 gtg Val1 gt c Val1 1 ctg Leu gac Asp ga t Asp gaa Giu ate Ile ggt Gi y ga c Asp ggc Gi y gag Gi u 145 tcc Se r acc Th r a cc Th r gag Glu gag Glu gcc Aia ccc Pro ga t Asp ggt Gly atg Met gga Gly 130 atc Ile atc Ile act Thr ggc Gly gcc Al a ggc Gi y tc Se r g cg Ala cgc Arg gtg Val1 100 atc Ile cg c Arg cg t Arg ct g Le u ttt Phe 180 gcc Ala aaa Lys cg a Arg ttt Phe cct Pro cca Pro ggc Gly cgc Arg att Ile cg t Arg ggt Gi y 165 gtt Val gtg Val1 115 163 211 259 307 355 403 451 499 547 595 643 691 739 ate aag gaa gtc acc ggc -le Lys Giu Val Thr Gly 200 eag atc act tcc Gin Ile Thr Ser 205 gea gac ctc ggt ggc Ala Asp Leu Gly Gly 210 WO 01/00805 WO 0100805PCTIBOOIOO926 gg t Gly ga a Giu 230 ttg Le u gag Giu aa c Asn gcc Al a acc Thr 310 cca Pro gcc Ala gt c ValI ggt Gly aco Thr 390 tat T yr t gg Trp atg Met gcg Ala 215 ga a Glu acc Thr atc I le cag Gin aac Asn 295 ttc Phe atg Met cgc Arg gtg Val1 ttg Le u 375 gt c Val1 gcc Ala cca Pro atc Ile atg caa aac Met Gin Asn aac att too Asn Ile Ser tat ttg gcg tcc tot Tyr Leu Ala Ser Ser 220 a tg Met gcc Al a got Al a cat His caa Gin 300 Giy tgc Cys tgc Cys tac Tyr gc a Ala 380 ttg Leu aag Lys gcc Ala Ct c Le u gtc Val1 Oct Pro ctg Leu gac Asp 285 gag G lu aag Lys atc Ile gac Asp ctg Leu 365 aag Ly s atc Ile aac As n gtg Val gaa Gl u 445 aag Lys gtg Val1 aac Asn 270 ct q Leu gag Giu gca Al a gao Asp gcc Ala 350 cot Pro ct~a Le u gt g Val ct C Leu atg Met 430 gc Al a otg Le u ga t Asp 260 gao Asp gao Asp atc Ile aao Asn aag Lys 340 ato Ile aag Lys gaa Glu gga Gly ttc Phe 420 gtc Vali cgt Arg Oct Pro 245 gaa Giu act Thr gao Asp acot Thr caa Gln 325 ggo Gly ttc Phe gto Val tog Ser g ca Ala 405 gca Ala gt g Val1 gaa.

Glu 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 14 11 1459 WO 01/00805 tac atg aag aaa ctg ttc atg gac ttc Tyr Met Lys Lys Leu Phe Met Asp Phe 455 460 cca tat gtg gcc gcc gag cgt ggt tac Pro Tyr Val Ala Ala Glu Arg Gly Tyr 470 475 .gca gjag ace cgt ttg gtg ctt cgc cga Ala Glu Thr Arg Leu Val Leu Arg Arg 490 aag get gtg cga gac etc gac aag aag Lys Ala Val Arg Asp Leu Asp Lys Lys 505 510 taacgtceaa agaattatcc aga, <210> 220 <211> 516 <212> PRT <213> Corynebacterium glutamicum <400>' 220 Leu Ser Asn Thr Thr Thr Ala Glu Lys aac Asn atg Met ca g Gin a tg Met a tg Met atc Ile ctg Leu c cg Pro 515 PCTIBOO/00926 age 1507 Ser cet 1555 Pro 485 acc 1603 Th r 1648 1671 1 Leu Asp Asp Glu Ile Gly Asp Gi y Glu 145 Ser Thr Ile Glu Gly Asp Gly Ser Al a 115 Ala Ala Met Asp Ala Gly Ser Ala Arg Val1 100 Ile Arg Arg Leu Phe 180 5 Lys Arg Phe Pro Pro Gly Arg Ile Arg GI y 165 Val1 Pro Thr Glu Se r Al a Thr Gly Asp 135 Le u Ser Gly Le u 10 Glu Gln Al a Val1 Al a 90 Arg Val1 Thr Se r Gly 170 Gly Ala Lys Leu Pro Gl y Val1 Val Asp Tyr Gln Pro 175 Tyr Arg Arg Leu Asp Arg Tyr Met Ser Ser Ile 160 Val' Val1 WO 01/00805 PCT/IB00/00926 Thr Gly Pro Ala Val Ile Lys Glu Val Thr Gly Glu Gin Ile Thr Ser Ala Tyr 225 Leu Ala Met Lys Pro 305 Val Ala Ile Asp Ala 385 Ala Asp Ala Pro Glu 465 Ala Arg 195 Leu Ala Phe Thr Asp 275 Phe Leu Ala Asp Ile 355 Glu Val Gly Asn Ala 435 Gin Met Ile Leu 200 205 Gly Ser Leu Asp 260 Asp Asp Ile Asn Lys 340 Ile Lys Glu Gly Phe 420 Val Arg Thr Glu Glu Gly Ser Pro 245 Glu Thr Asp Thr Gin 325 Gly Phe Val Ser Ala 405 Ala Val Glu Ser Pro 485 Ala Gin 215 Glu Glu Thr Cys Ile Ala Gin Gly 280 Asn Leu 295 Phe Ala Met Asp Arg Phe Val Asp 360 Leu Ile 375 Val Pro Ala Val Pro Thr Ile Gin 440 Met Lys 455 Tyr Val Glu Thr Gin Asn 235 Pro Glu Met Ile Asp 315 Gly lie Gly Gly Ser 395 Ser Ile Gin Phe Glu 475 Val Asn 220 Met Ala Ala His Gin 300 Gly Cys Cys Tyr Ala 380 Leu Lys Ala Leu Met 460 Arg Leu Gly Val Pro Leu Asp 285 Glu Lys Ile Asp Leu 365 Lys Ile Asn Val Glu 445 Asp Gly Arg Ile Asp Phe 255 Ser Leu Tyr Val Ala 335 Tyr Gly Ala Arg Thr 415 Gly Ala Tyr Ile Ala 495 Ser Leu 240 Ala Phe Asp Ala Gly 320 Asp Asn Val Phe Lys 400 Gly Ala Pro Asp Asp 480 Val Thr Lys Ala Val Asp Leu Asp Lys Lys His Thr 510 500 Ile Met Pro Met WO 01/00805 515 PCTIBOO/00926 <210> 221 <211> 1635 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (1612) <223> RXA00870 <400> 221 caagacggcg atgtcgccgc cqctgttgat accgcagcgc caacaattca cttcgcagag catttaagga atttacacac gacttgttca cacagatatt atg tct gaa cca caa 11 Met Ser Glu Pro Gin acc Thr aag Lys gtt Val1 acc Thr gct Al a ctg Leu atg Met ttc Phe att Ile agc Ser 150 gca atc Ile acc Thr gcg Ala aag Lys gtg Vali gcg Al a ggt Gly c ca.

Pro ga t Asp 135 ccg Pro atc tcg Ser gct Ala ctg Le u gct Al a ctt Leu gag Glu qa a Glu cac His 120 gtg ValI ttc Phe gct att Ile tac T yr cag Gin acg Thr ttc Ph e 75 act Th r cgc Ar g aaa Lys t tg Leu cc t Pro 155 aac ga c Asp aat As n gaa Glu t gg T rp cqt Arg gca Ala ggc Gly ggt Gly a agc Lys 140 gcg Ala gca gg c Gly cct Pro gag Gi u ggc Gi y gag Giu gag Glu cag Gin gcg Al a 125 cag Gin atg Met gt t tcc Ser gcc Aia t ct Ser cgc Arg ggt Giy ga t Asp 100 acc Thr acc Thr atc Ile cca Pro aag gg c Gly aat Asn g cc Aia ca a Gin gag Gi u gcc Al a ggt Gly ggc Gl y atc Ile atc Ile 165 ga t WO 01/00805 WO 0100805PCT1J1BOO/00926 Ala Ile Ala Ala Gly Asn Ala Val Ile Leu Lys Pro Ser Glu Lys Asp CC t Pro cca Pro ggt Gly acc Th r 230 cg C Arg ga t Asp ggc Gly ga a Giu acc Thr 310 ctg Leu gtc Val aac Asn ctt Le u ttc Phe 390 att Ile gca Ala ggC Gly 200 Ctg Leu atc Ile cag Gin ga t Asp gc C Ala 280 gt Val1 cgc Arg cca Pro atc Ilie 9CC Ala 360 gac Asp C Cg Pro ct g Leu gcg Al a 185 g ta Val1 aac Asn 9 ca Al a 9 cg Ala ctg Leu 265 ggt Gly gcc Ala atc Ile cta Leu g ct Ala 345 gta ValI gac Asp gtc Val atc Ile 170 ct g Le u ttc Phe agc Se r aag Lys t tg Leu 250 ga t Asp gag Glu gac Asp ggc Gly aic Ilie 330 gag Glu gac Asp atc Ile ci c Leu a ac As n tgg Trp aac Asn cci Pro tac Tyr 235 99 c Giy ci g Leu cg i Arg gag Giu aac Asn 315 acc Thr gcc Ala ggg Gly cca Pro t Ct Se r 395 tcc Ser a tg Met gtg ValI gat Asp 220 atc Ilie ggc Giy gitt Val t gc Cys ctc Le u 300 ggt Gly g ac Asp gac Asp cac His ctc Leu 380 gtc Val1 gg t Gly gct Ala ctC Leu 205 gtc Val1 tac Tyr g cg Ala gcc Al a atg Met 285 ati Ile gcc Ala gt c Val ggc Gly gag Giu 365 a cg Thr gt t Val1 ga a Glu cag Gin 190 ca g Gin ict Ser gag Glu aag Lys gat Asp 270 gci Ala gag Giu Gly cac His gcc Ala 350 gag Giu tic Phe cg t Arg ttc Phe 175 atc Ile 99 C Gi y gcg Ala act Thr aac As n 255 ca g Gin gt t Vali aag Lys gac Asp cgc Arg 335 aag Lys ggc Gly cgc Arg gt c Val1 Giy tgg T rp ga c Asp att Ile icc Se r 240 ca c His gca Ala tci Se r atc Ile gag Giu 320 gac Asp atc Ilie ttc Phe 9CC Ala g ca Ala 400 aac Asn aag L ys a ag L ys tcc Se r 225 gcg Ala atg Met aic Ile gtg Val1 aag Lys 305 cag Gln aag L ys aic Ile tc Phe tac Tyr 385 tc Ser gga Gi y gaa Giu cig Leu 210 tc Phe aag Lys c ig Leu aac Asn gtc Val1 290 gag Giu Giy gic Val1 gtg ValI tic Phe 370 acc Th r tic Ph e acc Thr gct Ala 195 gC t Ala gig Val aac As n gig Val 9 ca Al a 275 tig Le u Cg C Arg gag Gi u gct Al a ga C Asp 355 gg C Gly gaa Glu ga C Asp gca Ala 180 ggi Gi y gt ValI ggt Gi y Gly Ct 9 Leu 260 99 i Gly gci Ala atc Ile C C9 Pro ici Se r 340 999 Gly cci Pro gaa Glu gag Giu aic Ile cii Leu gat Asp icc Se r aag Lys 245 cca Pro tac Tyr at Ile gac Asp ca C His 325 tat Tyr cg i Arg a cg Thr aic Ile gca Ala 405 ttc Phe 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 WO 01/00805 .PCT/IBOO/00926 410 415 420 acc aac gat ggt gga gcg gca cgc cgc ttc cag cat gag atc gaa gtg 1411 Thr Asn Asp Gly Gly Ala Ala Arg Arg Phe Gin His Giu Ile Giu Val 425 430 435 ggc atg atc ggc atc aac gta cca atc cca gtg cct gtt gcg tac cac 1459 Gly Met Ile Gly Ile Asn Vai Pro Ile Pro Val Pro Val Ala Tyr His 440 445 450 tcc ttc ggt ggt tgg aag aac tcc ctc ttc ggt gac gcc aaq gca tat 1507 Ser Phe Gly Gly Trp Lys Asn Ser Leu Phe Gly Asp Ala Lys Ala Tyr 455 460 465 ggc act caa ggt ttt gat ttc ttc acc agg gaa aag gcg atc acc agc 1555 Gly Thr Gin Gly Phe Asp Phe Phe Thr Arg Giu Lys Ala Ile Thr Ser 470 475 480 485 cgt tgg ctc gac cca qca. acc cac ggt ggc att aac ctc ggt ttc cca 1603 Arg Trp Leu Asp Pro Ala Thr His Gly Gly Ile Asn Leu Gly Phe Pro 490 495 500 cag aac gat taattgaagg agagcacagg act 1635 Gin Asn Asp <210> 222 <211> 504 <212> PRT <213> Corynebacterium glutamicun <400> 222 Met Ser Glu Pro Gin Thr Ile Ser His Trp Ile Asp Gly Ala Ile Ser 1 5 10 Pro Ser Thr Ser Gly Lys Thr Ala Pro Val Tyr Asn Pro Ala Thr Gly 25 Gin Val Thr Ala Asn Val Ala Leu Ala Ser Gin Giu Giu Ile Asp Ala 40 Thr Ile Ala Ser Ala Thr Lys Ala Ala Lys Thr Trp Gly Asn Leu Ser 55 Ilie Ala Lys Arg Gin Ala Val Leu Phe Asn Phe Arg Glu Leu Leu Asn 70 75 Ala Arg Lys Gly Giu Leu Ala Glu Ile Ile Thr Ala Giu His Gly Lys 90 Val Leu Ser Asp Ala Met Gly Giu Ile Leu Arg Gly Gin Giu Val Val 100 105 110 Giu Leu Ala Thr Gly Phe Pro His Leu Leu Lys Gly Ala Phe Asn Giu 115 120 125 Asn Val Ser Thr Gly Ile Asp Val Tyr Ser Leu Lys Gin Pro Leu Gly 130 135 140 Val Val Gly Ile Ile Ser Pro Phe Asn Phe Pro Ala Met Val Pro Met WO 01/00805 PCT/IB00/00926 145 150 155 160 Trp Phe Phe Pro Ile Ala Ile Ala Ala Gly Asn Ala Val Ile Leu Lys 165 170 175 Pro Ser Glu Lys Asp Pro Ser Ala Ala Leu Trp Met Ala Gin Ile Trp 180 185 190 Lys Glu Ala Gly Leu Pro Asp Gly Val Phe Asn Val Leu Gin Gly Asp 195 200 205 Lys Leu Ala Val Asp Gly Leu Leu Asn Ser Pro Asp Val Ser Ala Ile 210 215 220 Ser Phe Val Gly Ser Thr Pro Ile Ala Lys Tyr Ile Tyr Glu Thr Ser 225 230 235 240 Ala Lys Asn Gly Lys Arg Val Gin Ala Leu Gly Gly Ala Lys Asn His 245 250 255 Met Leu Val Leu Pro Asp Ala Asp Leu Asp Leu Val Ala Asp Gin Ala 260 265 270 Ile Asn Ala Gly Tyr Gly Ala Ala Gly Glu Arg Cys Met Ala Val Ser 275 280 285 Val Val Leu Ala Ile Glu Ser Val Ala Asp Glu Leu Ile Glu Lys Ile 290 295 300 Lys Glu Arg Ile Asp Thr Leu Arg Ile Gly Asn Gly Ala Gly Asp Glu 305 310 315 320 Gin Gly Glu Pro His Leu Gly Pro Leu Ile Thr Asp Val His Arg Asp 325 330 335 Lys Val Ala Ser Tyr Val Asp Ile Ala Glu Ala Asp Gly Ala Lys Ile 340 345 350 Ile Val Asp Gly Arg Asn Cys Ala Val Asp Gly His Glu Glu Gly Phe 355 360 365 Phe Phe Gly Pro Thr Leu Ile Asp Asp Ile Pro Leu Thr Phe Arg Ala 370 375 380 Tyr Thr Glu Glu Ile Phe Gly Pro Val Leu Ser Val Val Arg Val Ala 385 390 395 400 Ser Phe Asp Glu Ala Ile Glu Leu Ile Asn Ser Gly Glu Phe Gly Asn 405 410 415 Gly Thr Ala Ile Phe Thr Asn Asp Gly Gly Ala Ala Arg Arg Phe Gin 420 425 430 His Glu Ile Glu Val Gly Met Ile Gly Ile Asn Val Pro Ile Pro Val 435 440 445 Pro Val Ala Tyr His Ser Phe Gly Gly Trp Lys Asn Ser Leu Phe Gly 450 455 460 Asp Ala Lys Ala Tyr Gly Thr Gin Gly Phe Asp Phe Phe Thr Arg Glu 465 470 475 480 WO 01/00805 PCTIIBOO/00926 Lys Ala Ile Thr Ser Arg Trp Leu Asp Pro Ala Thr His Gly Gly Ile 485 490 495 Asn Leu Giy Phe Pro Gin Asn Asp 500 <210> 223 <211> 1305 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1282) <223> RXA01260 <400> 223 ctaaacgtgg gctgcattcc ttccaaagtc tctgatcaaa ctttacccat gagaagaaqa ccttcqgcat caatggcgaa aacgctgaag ttgcccatac gct Al a cat His aac Asn ggC Gly gtc Val1 gaa Giu ggt Gi y ggt Gi y 120 ca C His tac T yr tt C Phe aag Lys aac Asn gag Giu gca Al a 105 gta Vali aag Lys ttg Le u aag Lys acc Thr acc Thr ca g Gin ggc Giy ga t Asp tcc Ser aag Lys gct Aia acc Th r cgt Arg ct t Le u att Ile acc Thr cgt Arg aaq Lys aag Lys 45 ttt Phe ggc Gly aac As n gga Giy gtc Val 125 gt t ValI 15 aag Lys ct t Le u ga c Asp ga c Asp gtt Vali 95 gaa Giu gag Giu tcc Ser atc Ile gag Giu tgc Cys ttc Ph e g cg Ala ttc Phe ttc Phe gtg Vali 1 gac Asp atc Ile gtc Vali atc Ile t ca Se r cca Pro gc c Ala atg Met Thr aag Lys gaa Giu acc Thr atc Ile gag Gi u aag Lys tac Tyr ga t Asp 130 acc ttc aac tat Phe Asn atc gtt Ile Vai att cat Ile His gac ggt Asp Gly gca acc Ala Thr aac gtt Asn Vai aag atg Lys Met 100 gtt ctt Val Leu 115 cgt gtg Arg Val Tyr gga Gly gg t Gly aag Lys gg t Gly gtg Val gt c Val ggt Gly ctt Leu 115 163 211 259 307 355 403 451 499 cca aat gaa gat Pro Asn Giu Asp 135 gaa gtc tcc aag gtt att Giu Val Ser Lys Vai Ile 140 aag gcc tac aag Lys Aia Tyr Lys WO 01/00805 WO 0100805PCT[LBOO/00926 aag atg Lys Met 150 gac aac Asp Asn aag aca Lys Thr cgt cca Arg Pro acc gag Thr Giu 215 gat ggc Asp Gly 230 cac gt.c His Val gca gaa Ala Giu ttc tgc Phe Cys aag gag Lys Giu 295 tct gca Ser Ala 310 aag atc Lys Ile gtt gga Val Gly aac tgg Asn Trp cca acg Pro Thr aag ctt ctt cct Leu 155 gtc Val act Thr gga Gly atc Ile atc Ile 235 ca g Gin ctt Le u gt t Vai gat Asp gca Aila 315 gca Al a tca Se r act Thr gca Leu Pro gag gtt Giu Val gtt gat Val Asp ttt ggt Phe Gly 205 gag atc Giu Ile 220 ggt gac Giy Asp qgc att Gly Ile ggt gat Gly Asp tct tcc Ser Ser 285 cgt gag Arg Glu 300 gtt ggc Val Gly gaa ttc Glu Phe gag ctc Glu Leu gaa gag Giu Giu 365 gtt aag acc act Thr Thr aag aag Lys Lys gtt tcc Val Ser act ggc Thr Gly 210 atg cgt Met Arg 225 aag ctt Lys Leu gag act Giu Thr atg cca Met Pro acc gaa Thr Glu 290 gct tcc Ala Ser 305 act gat Thr Asp ctc ggt Leu Gly ttg gtg Leu Val agc gtc Ser Val 370 cac ggt His Gly gct Al a ggc Gly gtt Val1 195 gtt Val1 acc Th r ca g Gin att Ile cg t Arg 275 gag Giu ttc Phe ggt Gly gca Ala ct t Le u 355 cat His atc Ile gtt Val1 tct Ser 180 ggt Gi y a ag Lys aac Asn Ct t Le u gct Ala 260 gca Ala cag Gin cca Pro ttc Phe cac His 340 g ct Al a att Ile tct Ser 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1305 Ala Val Lys cac atg atc aac ttC tagaatccac ctcgttggcc ctg WO 01/00805 His Met Ile Asn Phe 390 <210> 224 <211> 394 <212> PRT <213> Corynebacterium glutamicum <400> 224 Val Thr Phe Asn Tyr Glu Asp Ala His PCTIBOO/00926 Arg Ser Arg Gly Val Ser 1 Asp Ile Val Ile Se r Pro Al a Met Ala 145 Thr Lys Val1 Th r Met 225 Lys Glu Ly s Glu Thr Ile Glu Lys Tyr Asp 130 Lys Thr Ly s Ser Gly 210 Arg Le u Thr His Asn Gly Val1 Glu Gi y Gi y 120 Gi u GI y Gly Gu Arg 200 Arg Ile Ala Thr Tyr Phe Lys As n Glu Al a 105 Val1 Asp Val1 Asp Thr 185 Val1 Gly Tyr Glu Gin 265 WO 01/00805 Met Pro Arg Ala Thr Phe Cys Asn Pro Gin Val 275 280 Thr Giu Glu Gin Ala Lys Giu Lys Trp Pro Asp 290 295 Ala Ser Phe Pro Phe Ser Ala Asn Gly Lys Ala 305 310 315 Thr Asp Gly Phe Ala Lys Ilie Val Ala Asp Ala 325 330 Leu Gly Ala His Leu Val Gly Ala Asn Ala Ser 340 345 Leu Val Leu Ala Gin Asn Trp Asp Leu Thr Thr 355 360 Ser Val His Ile His Pro Thr Leu Ser Giu Ala 370 375 His Gly Ile Ser Gly His Met Ile Asn Phe 385 390 <210> 225 <211> 294 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <222> (101)..(271) <223> RXA01261 <400> 225 gtgggtgttt ttcattttct tccactctaa aattaaqtat atgcacgaca atgacccact aaacacgtat ccttgaatgc Ph e Ile Leu Gi y Ile 350 Ile Glu GI y Lys Ala Giu 335 Asn Se r Ala PCT/LBOO/00926 Tyr Val1 Giu 320 Leu Glu Arg Ala gac gta g~ Asp Val V, cgt gca g, Arg Ala A tgg ggt g, Trp Gly G.

gat caa a, Asp Gin L, <210> 226 <211> 57 ctc gga gcc ggc ccc ggt ggc Leu Gly Ala Gly Pro Gly Gly 15 ctt ggc aag aag gtt gct gta Leu Gly Lys Lys Vai Aia Val tgc cta aac gtg ggc tgc att Cys Leu Asn Val Gly Cys Ile tgaagttgcc cataccttta ccc ggaaaaccaa ccgcacccgg gtq act gaa cat tat Val Thr Giu His Tyr 1 tat gtc tcc gcc atc Tyr Val Ser Ala Ile att gag aag cag tac Ile Giu Lys Gin Tyr cct tcc aaa gtc tct Pro Ser Lys Val Ser WO 01/00805 PC <212> PRT <213> Corynebacterium glutamicum <400> 226 Val Thr Glu His Tyr Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly 1 5 10 Tyr Val Ser Ala Ile Arg Ala Ala Gln Leu Gly Lys Lys Val Ala Val 25 Ile Glu Lys Gln Tyr Trp Gly Gly Val Cys Leu Asn Val Gly Cys Ile 40 Pro Ser Lys Val Ser Asp Gln Lys Arg <210> 227 <211> 555 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (532) <223> RXA01136 <400> 227 ccctcgcaac accgacggta gcctcttgga aatggcgcgc atcgtgcgtg agctttcc TIiBOO/00926 cg ctaggacctg tctactgcct tcactttgtg cgaacaccgc atg acc ttg Met Thr Leu gat tac Asp Tyr ttc Phe gaa Glu gag Glu ggt Gly gaa Glu ct a Le u t ca Ser ggc Gly cgc Arg tgt Cys gt t Val att Ile ttg Le u Ct C Le u act Thr act Thr ggt Gly agt Ser gtg Val1 gg C Gly ggt Gly 1 ttg Leu gcg Ala g ca Ala gct Ala gca Ala gt c Val1 tgc Cys g ct Ala agt Ser acc Th r tcc Se r gag Glu acc Thr gct Ala g ca Ala gca Ala agg Arg aac Asn g gc Gly ctc Le u tgg Trp 115 WO 01/00805 PCTIBOOIOO926 ttt gag gat gtc ttc att cct ggg gct cag att cta agc cac gat ttc 499 Phe Giu Asp Val Phe Ile Pro Gly Ala Gin Ile Leu Ser His Asp Phe 120 125 130 ctt acc ttg tgg cat cgg tgc gcc caa cct tcg tgatcctacg gatctccgaa 552 Leu Thr Leu Trp His Arg Cys Ala Gin Pro Ser 135 140 tac 555 <210> 226 <211> 144 <212> PRT <213> Corynebacterium glutamicum <400> 228 Met Thr Leu Asp Tyr Phe Lys Ala Ser Gly Thr Asp Tyr Ala Leu Gly 1 5 10 Leu Ala Ala Glu Ser Glu Gly Ala Arg Arg Thr Gly Ile Thr Gly Met 25 Ala Ser Ala Phe Lys Glu Phe Ala Gly Cys Gly Giu Ile Asp Leu Glu 40 Ala Thr Arg Val Glu Gly Gly Leu Lys Vai Ser Giy Lys Leu Arg Trp 55 Ala Ser Asn Leu Cys Giu Asp Pro Vai Ile Val Pro Ala Ala Lys Thr 70 75 Ala Glu Gly Leu Gin Leu Leu Phe Ala Leu Gly Ala Giu Thr Glu Gly 90 Val Thr Leu Gly Ser Ser Leu Ala Leu Leu Gly Leu Asn Ala Thr Ala 100 105 110 Cys Ala Trp Val Ser Phe Glu Asp Val Phe Ile Pro Gly Ala Gin Ile 115 120 125 Leu Ser His Asp Phe Leu Thr Leu Trp His Arg Cys Ala Gin Pro Ser 130 135 140 <210> 229 <211> 1140 <212> DNA <213> Corynebacterium qiutamicum <220> <221> CDS <222> (1117) <223> RXN00559 '400> 229 cccttcaatc cagtctttga cggccaatac ggcttgccgg gtttccagcg gatcaatcct WO 01/00805 catgaagcat cagcctagta cgaaccgtta aagtgtccat PCTJIBOO/00926 atg tct gat aat ccg 115 Met Ser Asp Asn Pro cat His cg t Arg gt t Val1 tg t Cys cac His cgt Arg gag Giu ga c Asp acc Thr ttg Leu 150 gag Gi u att Ile gtc Val1 tgc Cys gag Giu t tc Phe gg c Gly gct Ala att Ile t cc Se r gt g Val1 tgc Cys cca Pro 135 ga a Glu atg Met acc Thr cac His acc Thr 215 aat Asn atg Met ggt Gly acc Thr cac His cgc Arg ctt Leu ttg Le u 120 gtt Val1 gc t Ala ga a Giu cg c Ar g ggt Gly 200 atg Met ccc Pro g ct Ala ggc Gly ca g Gin ttc Phe att Ile tct Ser 105 gtt Val1 cc t Pro gct Ala a ag Lys ttc Ph e 185 ggc Gi y gag Glu cg t Arg gcc Ala cga Arg t gg T rp act Thr gcg Ala g cg Al a att Ile t ca Ser aac Asn ca g Gin 170 t tg Leu act Thr t gg Trp gag Glu cct Pro gtc Val1 t ct Ser cgc Arg 75 atg Met gat Asp ttc Phe ttt Phe t cc Se r 155 a cg Thr gct Al a gcc Ala t ct Se r aat Asn act Thr ctg Leu gg a Gly 60 cct Pro act Thr cc t Pro gt g Val1 acc Thr 140 cgc Arg tac Tyr aag Ly s atg Met gg t Gly 220 cca Pro gac Asp ga a Glu 45 acc Th r att Ile Gi y cgc Arg gcg Ala 125 c ct Pro at c Ile aac Asn cca Pro gaa G lu 205 a ac As n cac cgc His Arg 15 gtt ttg Vai Leu 30 t gg atc Trp Ile tac tgt Tyr Cys ccc tct Pro Ser cgt tcc Arg Ser 95 gat ggc Asp Gly 110 aag gac Lys Asp aag aat Lys Asn ggg ctg Gly Leu gga cct Gly Pro 175 aca gat Thr Asp 190 tgg atc Trp Ile cac acc His Thr tcc Ser atg Met ga t Asp gtc Val1 Gly 80 t cc Ser aac As n acc Thr gaa Giu cgc Arg 160 tct Se r atc Ile ga t Asp gt t Vali cca Pro gct Ala aag Lys act Thr cac His atg Met tac Tyr gca Al a ga a Glu 145 aa g Lys gag Giu aac As n gag Glu gcg Al a 225 ga a Giv ggt Gi y g ct Ala gct Ala atg Met cac His acc Thr act Thr 130 gag Glu gct Ala gcc Ala t gg Trp gcg Al a 210 gt t Val1 gt c Val1 agc Ser gct Ala tat Tyr gtc Val1 atc Ile cgt Arg 115 gqt Gly cag Gln att Ile cc t Pro ggt Gly 195 gg t Gi y tat Tyr gtc Val1 cat His tat Tyr gt t Val1 gag Glu gtg Val 100 g cg Ala cg c Arg cgc Arg ga a Giu cgt Arg 180 g gc Gly gct Ala gct Ala ctt Leu gg c Gly gct Ala gg t Gly gt g Val1 aat As n tgt Cys gct Al a gt g Val1 gcg Al a 165 ttg Leu aag Lys gcg Ala ggt Gly WO 01/00805 PTLOIO2 PCTABOO/00926 gg t Giy 230 gac Asp cac His gc t Al a cct Pro ttg Le u 310 cca atc cgc ttc tac cag ccc att cag atc Ile Arg Phe Tyr Gin Pro Ile Gin Ile gcc Ala gtc Val1 att Ile t tg Leu 295 gct Ala atg cgc Arg cgt Arg cac His 280 cct Pro gag Glu c cg atg Met 250 ggt Gly a cg Thr cca Pro gca Ala ttc 235 cg t Arg ga t Asp gtg Val1 ca g Gin aac Asn 315 cag ggt qac Gly Asp 240 tcc atg Ser Met cgt gct Arg Ala att gat Ile Asp acc cct Thr Pro 305 ctg cgt Leu Arg 320 ctg cag ctc att gag Leu Ile Giu cag atg tcc Gin Met Ser 260 gag cta gaa Glu Leu Giu 275 gtc gac gga Val Asp Giy 290 gag gat atc Giu Asp Ile gct gat tac Ala Asp Tyr atc gac Ile Asp Pro Met Pro Leu Phe Gin Arg Arg Val Pro Leu Gin 330 335 tagttagacc cgaaaaagcc ccc <210> 230 <211> 339 <212> PRT <213> Corynebacterium giutamicum <400> 230 Met Ser Asp Asn Pro His Glu Asn Pro 1 Pro Giu Vai Val Leu Arg Phe Met Ala Ala Gly Ser His Gly Val Gly Gly Gly Lys Ala Ala Tyr Ala Cys Ala Thr Gin Thr Ala Tyr Val Giy His Ile His Phe 70 His Met Val Giu Val Arg Ser Arg Ile Met His Ile Val Asn Giu Val Leu Ser 100 105 Tyr Thr Arg Ala Cys Asp Cys Leu Vai 115 120 gtq Val 245 atc Ile acc Thr gag Giu cag Gin acc Th r 325 Se r Met Asp Val1 Gly Ser Asn Thr 835 883 931 979 1027 1075 1117 1140 WO 01/00805 WO 0100805PCTA~BOOIOO926 Ala Thr Gly Arg Ala Thr Pro Val Pro Ser Gi u 145 Lys Giu Asn Glu Ala 225 Asp Met Al a Asp Pro 305 Arg Gln 130 Glu Ala Ala T rp Ala 210 Val1 Leu Gin Glu Vai 290 Giu Ala Ile Gin Ile Pro Gly 195 Gi y Tyr Ile Met Leu 275 Asp Asp Asp Asp Arg Glu Ar g 180 GI y Al a Ala Giu Ser 260 Glu Gly I le Tyr Le u 150 Glu Ile Val1 Cys Gly 230 Asp His Ala Pro Leu 310 Pro 135 Giu Met Thr His Thr 215 Ile Al a ValI Ile Leu 295 Ala Met Al a Glu Arq Gi y 200 Met Arg Arg Arg His 280 Pro Glu Pro Al a Lys Phe 185 Gly Glu Ph e Met Ala 265 Ala Ala His Le u Pro Ile Asn Pro Glu 205 Asn Ile Asp His Tyr 285 Val1 Le u Arg Lys Gly Gi y Th r 190 Trp His Gin Lys Arg 270 Le u Pro Arg Val As n Le u Pro 175 Asp Ile Th r Ile Arg 255 Gly Gly Arg Asp Pro 335 Glu Arg 160 Ser Ile Asp Val1 Gly 240 Se r Arg Ile Th r Le u 320 Le u <210> 231 <211> 313 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(313) <223> FRXA00559 <400> 231 cccttcaatc cagtctttga cggccaatac ggcttgccgg gtttccagcg gatcaatcct catgaagcat cagcctagta cgaaccgtta aagtgtccat atg tct gat aat ccg 115 Met Ser Asp Asn Pro 1 cat gag aat ccc cgt gag aat cca cac cgc tcc cca gaa gtc gtc ctt 163 WO 01/00805 His Glu Asn P cgt ttc atg g Arg Phe Met A gtt ggc ggt g( Val Gly Gly G.

tgt gct acc c~ Cys Ala Thr H: cac att His Ile ro Arg Glu ct gcC cct la Ala Pro gc cga gtc ly Arg Val ac ggg tct is Gly Ser Pro His Arg Ser 15 gac gtt ttg atg Asp Val Leu Met 30 gaa tgg atc gat Glu Trp Ile Asp 45 acc tac tgc qtc Thr Tyr Cys Val Pro gct Ala aa g Ly s act Thr PCTIBOO/00926 Glu Val Val Leu ggt agc cat ggc 211 Gly Ser His Gly gct gct tat gct 259 Ala Ala Tyr Ala gct tat gtt ggt 307 Ala Tyr Val Gly <210> 232 <211> 71 <212> PRT <213> Corynebacterium glutamicum <400> 232 Met Ser Asp Asn Pro His Glu Asn Pro Arg Glu Asn Pro His Arg Ser 1 5 10 Pro Glu Val Val Leu Arg Phe Met Ala Ala Pro Thr Asp Val Leu Met 25 Ala Gly Ser His Gly Val Gly Gly Gly Arq Val Leu Glu Trp Ile Asp 40 Lys Ala Ala Tyr Ala Cys Ala Thr His Gly Ser Gly Thr Tyr Cys Val 55 Thr Ala Tyr Val Gly His Ile <210> 233 <211> 802 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(802) <223> RXA01580 <400> 233 cggtaaacgc ctcattaaag tccaatgcca tgctcataac actaacagtt aaccgtgcgq tcaactttgc tccctatcct taaaaagccc acagaaaagg atg tat aag aat atg Met Tyr Lys Asn Met 1 cac att gtt gcc cat cgc ggt gqgqaa gat ctq cac ctc gaa aac acc His Ile Val Ala His Arg Gly Ala Glu Asp Leu His Leu Glu Asn Thr WO 01/00805 WO 0100805PCTJIBOOIOO926 atg acc gct Met Thr Ala atc cac gcc Ile His Ala gca gcg cgt Ala Ala Arg gcg cgc tta Ala Arg Leu tcc ccc gta Ser Pro Val atc caa gtg Ile Gin Vai tta ttg cag Leu Leu Gin 120 ttc atc gat Phe Ile Asp 135 cgc gtg gga Arg Vai Giy 150 gat tac atc Asp Tyr Ile gca cta aac Ala Leu Asn gtt ggc tgc Val Gly Cys 200 caa gct ggc Gin Ala Gly 215 cgc cct ccc Arg Pro Pro 230 <210> 234 <211> 234 ttc Phe acc Thr gt t Val agc Se r cc a Pro ga a Glu 105 aaa Lys gca Ala atc Ile ccg Pro gt g Vali 185 tgg T rp gt t ValI ct g Leu cag gct gcc gcg ccc Gin gct Al a gcc Al a gcc Al a a cc Thr atc Ile tac Tyr gca Al a ttg Leu ct a Leu 170 gcg Al a aca Thr gat Asp ctg Le u Al a gac Asp gcg Al a g Ala 75 ctg Le u aaa Ly s cca Pro ctg Le u cgc Arg 155 aaa Lys tca Ser att Ile tac Tyr Ala a at Asn cca Pro 60 caa Gin gag Glu t ct Se r gag Giu gt g Val1 140 ga t Asp aa t Asn att Ile.

cgg Arg ccc Pro 220 Al a ca g Gin 45 gat Asp atc Ile ga a Giu g cc Ala cac His 125 gaa Glu q cq Ala gtg Val1 gct Al a ga t Asp 205 act Thr Pro 30 gtc ValI tcc Ser aag Ly s gtt Val1 gg t Gi y 110 ct g Le u atc Ile t cc Ser ggc Gly gat Asp 190 ga a Giu gt t Val gct Ala gt c Val1 ctg Le u gag Gi u cta Le u 95 gca Al a gag Glu gtg Val1 atg Met gcg Ala 175 cta Le u aat As n agc Se r ga c Asp gtt Va 1 cac His ata Ile 80 ct c Leu gt t Val1 cgc Arg gat Asp gat Asp 160 atc Ilie cat His gcg Al a ga t Asp gct ttt gag Ala Phe Glu atc: cac gac Ile His Asp cgc gac acc Arg Asp Thr acg ctt atc Thr Leu Ile cag acg agc Gin Thr Ser cca gca gcc Pro Ala Ala 115 ctg ctg ttc Leu Leu Phe 130 cga ctg cca Arg Leu Pro 145 gat ctg cgc Asp Leu Arg ttg ccc tcg Leu Pro Ser acc aag gga Thr Lys Gly 195 ttt ggg atc Phe Gly Ile 210 ccc tct cgt Pro Ser Arg 225 ctg gat Leu Asp cgc acc Arg Thr ccg gtt Pro Val gac gga Asp Gly ctg ccg Leu Pro 100 gca gca Ala Ala atc agt Ile Ser gaa gct Giu Ala att ctt Ile Leu 165 tgg aaa Trp Lys 180 atc aag Ile Lys gca caa Ala Gln ttc tcg Phe Ser 211 259 307 355 403 451 499 547 595 643 691 739 787 WO 01/00805 <212> PRT <213> Corynebacterium glutamicum PCTIBOO/00926 <400> 234 Met Tyr Lys Asn Met His Ile Val Ala His Arg Gly Ala Giu Asp Leu 1 His Ala Ile Arg Thr Gin Pro Le u Arg 145 Asp Leu Th r Phe Pro 225 Leu Phe His Asp Leu Thr Al a Le u 130 Leu Le u Pro Lys Gly 210 Ser Asn Leu Arg Pro Asp Leu 100 Al a Ile Giu Ile T rp 180 Ile Ala Phe 5 Th r Asp Thr Val1 Gly Pro Ala Ser Ala Leu 165 Lys Lys Gin Se r Met Ile Ala Al a 70 Se r Ile Le u Phe Arq 150 Asp Al a ValI Gin Arg 230 Thr His Al a Arg Pro Gin Leu Ile 135 Val Tyr Leu Gly Ala 215 Pro Phe 25 Thr Val1 Se r Pro Glu 105 Lys Aia Ile Pro Val1 185 Trp Val Leu Gin Ala Ala Ala Th r 90 Ile T yr Al a Le u Le u 170 Ala Thr Asp Le u Al a Gin Asp Ile Glu Al a His 125 Glu Ala Val1 Ala Asp 205 Thr Pro Val Ser Lys Val1 Gly 110 Leu Ile Ser Gi y Asp 190 Glu Val1 Ala Val Le u Glu Le u Al a Glu Val Met Ala 175 Le u Asn Se r Asp Val1 His Ile Leu Val1 Arg Asp Asp 160 Ile His Ala Asp (210> 235 <211> 882 (212> DNA <213> <220> <221> <222> <223> (400> Corynebacterium giutamicun

CDS

(101)..(859) RXA02 677 235 WO 01/00805 ttagtgcagt gtatttattt ccgttcacgc tgcggggctg agtcgatagc aggtatataa aggccaggag agatgggttc PCT/IBO0100926 gtggtttgga gggatactag atg aaa gtc Met Lys Val atc gcg Ile Ala cac His cg g Arg ctg Le u cgc Arg ttg Le u act Thr ctt Le u gaa Giu cg c Ar g cg c Arg 150 cg t Arg ggg Giy aaa Lys ct 9 LeO( cg a Arg g cg Ala act Thr gtg Val1 ct 9 Leu tta Le u tat Tyr ga a Giu atc Ile 135 ctt Le u tgg Trp ttg Le u ggg Gly tgg Trp 215 ggt Gly gct Ala aaa Lys t cg Se r agc Ser aac Asn ata Ile atc Ile 120 cac His gct Ala ggt Gly t cg Se r tta Le u 200 gcg Ala tta Le u ct a Leu tgt Cys aa t As n ttg Le u aa t Asn gaa Giu 105 aca Thr atc Ile cca Pro aat As n ctg Leu 185 ccc Pro cgc Arg t cg Ser gag Glu gqc Giy ggt G ly aac Asn cta Le u acc Thr aaa Lys att Ile ca g Gin ccg Pro 170 gag Giu acc Thr qaa Gi u t ct Ser ct a Leu gaa Giu cgc Arg ttt Phe 75 tta Le u aag Lys atc Ile tct Se r ctt Leu 155 cqc Arg cgg Arg tat Tyr cag Gin cgc Arg ccg Pro gtg Val ggt Giy 60 gga Giy gat Asp cac His tta Le u ttt Phe 140 ga t Asp gat Asp gcg Ala ctt Leu ggc Gly 220 ttt Ph e att Ile gt t Val1 45 c~g Arg acc Th r att Ile cca Pro aaa L ys 125 g ca Ala cgc Arg g tg Vai aag Lys ttc Phe 205 gtC Val ccc Pro cat His 30 aac Asn gtg Vali aaa Lys ttt Phe atg Met 110 tat Tyr ctt Leu att Ilie cqc Arg atg Met 190 acc Th r gac Asp gaa Giu 15 gga Gly gtc Vali tcg Ser gaa Glu gag Giu 95 cgc Arg cgt Arg ccc Pro cat His tgc Cys 175 gat Asp gtc Val1 a tg Met tta Leu att Ile cat His cg t Arg acc Th r 80 ga t Asp tac Tyr ggg Gi y gcg Ala ct g Leu 160 ggt Gi y cca Pro gat Asp ctc Le u 1 aca Th r gaa Giu gac Asp t tg Le u cca Pro tat Tyr gcg Ala ctg Leu atg Met 145 cgc Arg gt a Val1 agg Arg aag Lys gc C Ala 225 gag Giu act Thr ccc Pro gac Asp gaa Gi u cca Pro gt C Vali acg Th r 130 tat T yr a gg Arg ccc Pro atg Met caa Gin 210 acc Thr tct Ser ga t Asp att Ile t tg Le u aaa Lys gat Asp atg Met 115 qaa Giu cgc Arg t cg Ser acc Thr att Ile 195 aaa Ly s aat As n gcg- Al a gt c Vali gt g Val1 gaa Giu gt g Val aag Lys 100 ct g Leu gac Asp atg Met t gg T rp ggt Giy 180 ggg Gi y gac Asp tat Tyr ttt Phe cgg Arg gat Asp t cc Se r ctt Le u ca c His gaa Giu cca Pro gct Ala gag Giu 165 t tg Leu gcg Ala atg Met c cg Pro 163 211 259 307 355 403 451 499 547 595 643 691 739 787 wo 01/0080s gac cgt gcg gcg Asp Arg Ala Ala 230 gct aat gcg cat Ala Asn Ala His PCTIiBOO/00926 gag ctt ttg aac gca cat ccc aag ccc gcc atg tac 835 Glu Leu Leu Asn Ala His Pro Lys Pro Ala Met Tyr 235 240 245 ggc aaa gaa gac taagaagaat gaacagctgc cgg 882 Gly Lys Glu Asp 250 <210> 236 <211> 253 <212> PRT <213> Corynebacteriom glutamicum <400> 236 Met Lys Val Ile Ala His Arg Gly Leu Ala Val1 Val1 Giu Val Lys 100 Leu Asp Met T rp Gly 180 Gb y Asp T yr Phe Arg Asp Se r Leo His Glu Pro Al a Glu 165 Leo Ala Met Pro Arq Leo Arg Leu Thr Leu Glu Arg A r c 150 Arg Gly Ly s Leo Asp 230 Ala Thr Val1 Leo Leo Tyr Glu Ile 135 Leo Trp Leo Gly Trp 215 Arg Ala L ys Ser Ser Asn Ile Ile 120 His Al a Gly Se r Leo 200 Ala Ala Se r 10 Glu Gly Gly As n Leo 90 Thr Lys Ile Gln Pro 170 Glu Thr Glu Glu Ser Leo Glu Arg Phe 75 Leo Lys Ile Se r Leo 155 Arg Arq T yr Gin Leo 235 Arg Pro Val Gly Gly Asp His Leo Ph e 140 Asp Asp Ala Leo Gly 220 Leu Glu Gly Val1 Ser Glu Glu Arg Arg Pro His Cys 175 Asp Val1 Met His Leo Ile His Arg Thr Asp Tyr Gly Ala Leo 160 Gly Pro Asp Leo Pro 240 WO 01/00805 Lys Pro Ala Met Tyr Ala Asn Ala His Gly Lys Glu Asp 245 250 PCTIIBOO/00926 <210> 237 <211> 1428 <212> DNA <213> Corynebacterium glutamicum <220> <221>' COS <222> (1405) <223> RXS01166 <400> 237 accgtaccca cagacacacc agaattaaca gaaacagact gaaaaacaac atcqctcgac atgcgcgtaa tcctaacccg cgcacactaa tgtggccgat atg ggc tac acc aac Met Giy Tyr Thr Asn ct c Le u cgc Arg cgc Arg gat Asp 99c Gly gag Giu tcc Ser cg c Arg acc Thr gga Gly 150 aac Asn a cg Th r atc Ile qt c Val1 a cg Th r ccc Pro gca Ala ccc Pro caa Gin 135 ctc Leu ga c Asp atg Met a tg Met cca Pro ttg Leu aac Asn ggt Gi y t gg T rp 120 ggc Giy aac Asn aca Thr tct Se r gg t Gly cat His gt C Val1 ccc Pro cga Arg 105 aca Thr gtc ValI gca Ala cgg Arg ccg Pro ctc Le u ttc Ph e aat Asn gcg Ala gcc Ala acc Th r gca Al a ttc Phe ttq Le u gt g Val c9q Arg gca Al a 60 gca Ala tcg Ser act Thr ccg Pro cac His 140 gac Asp cgc Arg cag Gin cgt Arg 45 gct Ala ccc Pro tac T yr g cg Ala cgt Arg 125 t gc Cys aaa Lys gcc Ala cag Gin 30 cct Pro gt t Vali t tg Leu cgt Arg acc Thr 110 ccc Pro ga t Asp ccc Pro ggg Gi y 15 gga Gly gat Asp cga Arg aaa Lys ttt Phe 95 gg c Gly gcg Ala ccc Pro ttc Phe t ca Ser agt Ser cqg Arg gtt Val1 gtt Vali 80 gaa Giu g ct Ala atc Ile tcc Ser gac Asp 160 1 tgt Cys gag Giu aaa Lys ccc Pro ttg Leu tac Tyr gt c Val gc c Aia cac His 145 gca Ala ga t Asp gca Al a cc t Pro ggt Giy Gly atc Ile ctc Le u atg Met 130 acc Thr atc Ile gcc Al a gtc Vai 9gc Gi y cta Le u gca Ala acc Th r ttt Phe 115 gct Ala tgc Cys att Ile tgg Trp ttt Phe ttt Phe aaa Lys cg9g Arg ggt Gly 100 tcc Se r cca Pro gcc Aia gc t Al a tgg Trp cgg Arg ga c Asp c ac.

His ggc Gi y gat Asp a ca Thr tcc Se r atc Ile tac Tyr 165 115 163 211 259 307 355 403 451 499 547 595 WO 01/00805 WO 0100805PCTIBOO/00926 gaa ctc ccc Glu Leu Pro ttc atc gat Phe Ile Asp gat tcc atc Asp Ser Ile 200 aga caa ctc Arg Gin Leu 215 tcc caa gga Ser Gin Gly 230 gca cct gat Aia Pro Asp gat ctc ttc Asp Leu Phe gtg att gCC Vai Ilie Ala 280 ttt gag gaa Phe Giu Giu 295 ctg aaa aat Leu Lys Asn 310 tac tcg tct Tyr Ser Ser att ctq qac Ile Leu Asp ctg ggt cgt Leu Giy Arg 360 aat gat gat Asn Asp Asp 375 tgg gcq gac Trp Aia Asp 390 cgt gtg cca gtc Vali tac Tyr 185 gct Aila Giy Gi y gt c Val1 cgc Arq 265 tac Tyr atc Ile atc Ile t cc Se r ga t Asp 345 gtg Vali gt c Vali aag Lys gga atc ctc tqg ttt cta Ile Leu Trp Phe Leu 170 ccc cgc gac Ccc gca Pro Arg Asp Pro Aia 190 gca gct aaa tcg ctt Ala Ala Lys Ser Leu 205 ctt tca ccg gaa gca Leu Ser Pro Giu Aia 220 ggc gcc act ggc tgg Gly Ala Thr Giy Trp 235 cgc cca aag gca gcg Arg Pro Lys Ala Ala 250 gtc ttg gac act gtc Vai Leu Asp Thr Vai 270 gcc atc gcg gga ctt Aia Ile Aia Giy Leu 285 atg tcg gtg tta aat Met Ser Vai Leu Asn 300 acc agc tgc gcg gga Thr Ser Cys Aia Gly 315 cgc ggg tgg aca cat Arg Gly Trp Thr His 330 ctg cca ctt gtt gtc Leu Pro Leu Vai Val 350 gcg cca gaa atc cca Ala Pro Glu Ile Pro 365 att ccc att gat ccc Ile Pro Ile Asp Pro 380 ggt acg cca ttg acc Giy Thr Pro Leu Thr 395 cgc aca ggt ctc aac gc t Al a 175 acc Thr ct c Le u ccg Pro gct Al a gt c Val 255 gac Asp gca Al a ga a Giu 99 t Gly ca g Gin 335 gct Ala gt g Vali att Ile tgg Trp cat cac His ggc Gly gac Asp ctt Leu qca Ala 240 gtg Val ggc Gly gtg ValI cgc Arg t cc Se r 320 ggc Giy gag Giu ctg Le u a 99 Arg cat His 400 ttC gga Gly gtc Val1 gcc Al a 99 c Gly 225 caa Gin 99 c Gly 99 a Gly aac Asn gga Gly 305 ttg Le u a cg Th r ttt Ph e t tg Leu gaa Giu 385 gaa Glu 999 gt9 Val t gc Cys t cc Ser ttc Phe tac Tyr 245 gt g Val1 gga Gly atg Met gtg Val1 99 C Gly 325 gac Asp aag Lys aaa Lys agc Ser c cg Pro 405 aga 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 SWO 01/00805 PCT/IB00/00926 Arg Val Pro Gly Arg Thr Gly Leu Asn His Phe Gly Pro Tyr Phe Arg 410 415 420 aac ctg gaa aag tac tcg gga tgg ctc ata gat cat ctt gtc 1405 Asn Leu Glu Lys Tyr.Ser Gly Trp Leu Ile Asp His Leu Val 425 430 435 tgagtgccgt tttaaaggct cgg 1428 <210> 238 <211> 435 <212> PRT <213> Corynebacterium glutamicum <400> 238 Met Gly Tyr Thr Asn Leu Asn Asp Thr Arg Val Leu Arg Ala Gly Ser 1 5 10 Cys Asp Ala Trp Trp Arg Thr Met Ser Pro Leu Val Gln Gin Gly Ser 25 Glu Ala Val Phe Arg Arg Ile Met Gly Leu Ser Arg Arg Pro Asp Arg 40 Lys Pro Gly Phe Asp Asp Val Pro His Phe Gly Ala Ala Val Arg Val 55 Pro Gly Leu Lys His Gly Thr Leu Val Asn Ala Ala Pro Leu Lys Val 70 75 Leu Gly Ala Arg Gly Glu Pro Asn Pro Ala Ser Ser Tyr Arg Phe Glu 90 Tyr Ile Thr Gly Asp Ser Ala Gly Arg Ala Ile Thr Ala Thr Gly Ala 100 105 110 Val Leu Phe Ser Thr Arg Pro Trp Thr Thr Gly Pro Arg Pro Ala Ile 115 120 125 Ala Met Ala Pro Ser Thr Gin Gly Val Ala Gin His Cys Asp Pro Ser 130 135 140 His Thr Cys Ala Ile Gly Leu Asn Ala Phe Tyr Asp Lys Pro Phe Asp 145 150 155 160 Ala Ile Ile Ala Tyr Glu Leu Pro Val Ile Leu Trp Phe Leu Ala His 165 170 175 Gly Leu Asp Val Val Phe Ile Asp Tyr Pro Arg Asp Pro Ala Thr Gly 180 185 190 Val Gin Tyr Tyr Cys Asp Ser Ile Ala Ala Ala Lys Ser Leu Leu Asp 195 200 205 Ala Val Leu Ala Ser Arg Gin Leu Gly Leu Ser Pro Glu Ala Pro Leu 210 215 220 Gly Leu Trp Gly Phe Ser Gin Gly Gly Gly Ala Thr Gly Trp Ala Ala 225 230 235 240 WO 01/00805 WO 0100805PCTIBOO/00926 Gin Leu Gin Asp Gly Ala Pro Pro 260 Gly Leu Leu Thr 275 Asn Ser Ser Glu 290 Gly Val Ser Asp 305 Leu Leu Ala Ser Thr Pro Leu Ala 340 Phe Gly Lys Gin 355 Leu Trp Gly Ser 370 Giu Leu Arg Asp 385 Giu Ser Gin Ala Gly Pro Tyr Phe 420 His Leu Val Ala Pro Asp Val Asp Val1 Phe Le u 310 Tyr Ile Leu Asn Trp 390 Arg As n Leu Ile Glu 295 Lys Ser Le u Giy Asp 375 Ala Val1 Le u Phe Al a 280 Giu As n Se r Asp Arg 360 Asp Asp Pro Giu Lys Asp Ala Val1 300 Cys Trp Leu Giu Ile 380 Pro Gly Gly Val 255 Asp Ala Giu Gly Gin 335 Ala Val1 Ile Trp His 415 Ile Val1 Gly Val Arg Se r 320 Gly Giu Le u Arg His 400 Phe Asp <210> 239 <211> 690 .c212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (667) <223> RXA00875 <400> 239 caatttggcg gcgctgctgg gtgatcgggg gccggttgtt ccgggtgtga ttggtgactg ggtgaggggc taacgcaaca atgtgtctaa gcttaggggc atg act act gag gtt 115 Met Thr Thr Glu Val 1 gaa ctg gtt gtt tta gct gat tcc gag ggc aat cct att ggt act gcg 163 Giu Leu Val Val Leu Ala Asp Ser Giu Gly Asn Pro Ile Giy Thr Ala 15 WO 01/00805 WO 0100805PCTIBOOIOO926 Ccg aaa gct acg Pro Lys Ala Thr tcc acc tat att Ser Thr Tyr Ile gca ttg tcg aag Ala Leu Ser Lys ggg cac cct ggt Gly His Pro Gly ggt gtc gat gag Gly Val Asp Giu gag att ctg cct Giu Ile Leu Pro 105 gtg gag tgg gag Val Giu Trp Giu 120 gaa ttt gtg gag Giu Phe Val Giu 135 gaa ccg cag aag Giu Pro Gin Lys 150 tct cca tgg cta Ser Pro Trp Leu atc ctg gaa gcg Ile Leu Giu Ala cac act aag gac acg cct ctg cat ttc gcg ttt Val His Thr Lys Asp Thr Pro Leu His Phe Ala Phe t tg Leu a ag Lys ccg Pro t tg Le u gat Asp ttg Leu cca Pro ctt Leu gtg Val1 170 aac As n aca Th r gat Asp 75 ggg Giy tac Tyr t gc Cys ct g Le u ttc Phe 155 ga t Asp cc g Pro t gg T rp 60 gag Giu ctg Le u cag Gin cc g Pro ga t Asp 140 ga c Asp ca g Gin ggg gag Giy Glu ggt gtg Gly Vai aac gcg Asn Ala gta gat Vai Asp 95 cgt gct Arg Ala 110 cac ctc His Leu gag gtg Giu Vai gtt gat Vai Asp agc gcc Ser Ala ctg ttg Leu Leu tgg acg Trp Thr gat gcg Asp Ala 80 tct ttc Ser Phe gtc gac Vai Asp gtg cgt Val Arg gag gag Giu Giu 145 gcc aca Ala Thr 160 cct gag Pro Giu gtg Val1 aac Asn att Ile ttg Leu gcg Al a tta Le u 130 ttc Phe c ca Pro ctg acg Thr t ct Se r cg t Arg ga t Asp t cc Ser 115 g cg Ala gag Giu ttt Phe cgc cgt Arg atg Met cg c Arg att Ile 100 gg c Giy gtg Val1 t gg Trp gtg Val caa cgt Arg tgt Cys a gg Arg caa Gin att Ile ggg Gly gcg Ala ttt Phe 165 gcc Ala Leu Arg Gin ttt gac gca gag Phe Asp Ala Giu taactaacta gtctagaagc ctt '210> 240 <211> 169 <212> PRT (213> Corynebacterium giutamicum <400> 240 Met Thr Thr Giu Val Giu Leu Val Val 1 5 Pro Ile Gly Thr Ala Pro Lys Aia Thr 25 Leu His Phe Ala Phe Ser Thr Tyr Ile 40 Leu Val Thr Arg Arg Ala Leu Ser Lys Leu Ala Asp Ser Giu Gly Asn 10 Val His Thr Lys Asp Thr Pro Leu Asn Pro Arg Gly Giu Leu Lys Thr Trp Pro Gly Val Trp Th r Ala Phe Asp Arg Giu 145 Thr Glu WO 01/00805 Asn Ser M Ile Arg A Leu Asp I 1 Ala Ser G 115 Leu Ala V 130 Phe Glu T Pro Phe V Leu Arg G.

1: et rg le 00 ly a31 rp a31 in PCT[IBOO/00926 Cys Arg Gin Ile Gly Ala Phe 165 Ala Gi u Le u Gin Pro Asp 140 Asp Gin Al a Th r Giu Tyr Val1 125 Asp Al a Leu Glu <210> 241 <211> 1308 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (101)..(1285) <223> RXA01292 (400> 241 tccgcgtccc tcgaaqactt aaaaaacgjgg cgggattttg qcctqgcaac caaagcaatg tgqaaagaag tgtttcaaaa gtc Val1 cq c Arg ggc Gi y ccc Pro tgc gga Gly gaa Giu acc Thr ct C Le u atc tqccgtcttt aaaaaagcca atg aca aaa gca gtg Met Thr Lys Ala Val 1 acc gca ctg ctc tta Thr Ala Leu Leu Leu aac gag cac ctc ggc Asn Giu His Leu Gly cgc tqg gac acc ggc Arg Trp Asp Thr Gly cac ttC ttt aag ctc His Phe Phe Lys Leu gtg cca ctt gaa ccc wo 01/00805 WO 0100805PCTAIBOO/00926 Cys Gly Thr Ser Ile gcc Ala gac Asp ggc Gly Ctg Le u tac Tyr 150 ct 9 Le u aaa Ly s cct Pro gt c Val1 g ca Al a 230 gtc Val1 Gly cac His gag Glu ttg Le u tac cgc Tyr Arg atc gat Ile Asp gcc aaa Ala Lys 120 gcc atc Ala Ile 135 tta agc Leu Ser tcg acc Ser Thr ctg cgc Leu Arg tcg cac Ser His 200 caa ggc Gln Gly 215 cta att Leu Ile agc gca Ser Ala tca gag Ser Glu cac acc His Thr 280 acg agc Thr Ser 295 gca ggc Ala Gly gtc Val1 gcg Ala 105 ctq Leu gac Asp

CCC

Pro t cg Ser caa Gln 185 acc Th r gtc Val1 t cg Se r atc Ile ttc Phe 265 gaa Glu t g Trp gt g Val1 atc Ile atg Met cgc Arg ggC Gi y gqc Gly ct a Le u 170 atc Ile ccg Pro tC Se r ctt Leu acc Thr 250 atc Ile acc Th r aag Lys aaq Lys Asp 75 80 Asp His Leu Asp Leu Val Pro Leu Glu Pro gac Asp gcc Al a acc Thr ttc Phe atg Met 155 aaa Lys tta Le u q cg Ala tat Tyr atc Ile 235 acc Th r gaa Glu caa Gin aac As n gg 9 Gly gac cac Asp His gag ctt Glu Leu tat atc Tyr Ile 125 ctc tac Leu Tyr 140 ctq cgc Leu Arg gtc aag Val Lys agc tac Ser Tyr ctc tat Leu Tyr 205 ccc cga Pro Arg 220 gac gac Asp Asp caa ggc Gin Gly gcc gat Ala Asp ctc cta Leu Leu 285 aaa caa Lys Gin 300 gag cac Giu His ggc Gi y ttc Phe 110 gac Asp acc Thr ctt Leu gtc Val

CCC

Pro 190 cac His 9gC Gly gcc Al a cga Arg atc Ile 270 cct Pro

CCC

Pro acc Thr gaa Glu 95 ga a Giu tcc Se r aac Asn tta Leui aac Asn 175 q Ca Al a ctc Le u 99 C Gly gt C Val1 aac Asn 255 gtg Val1 gCt Al a ggC Gly Ctg Le u ttC Ph e t CC Ser g CC Al a ttc Phe

CCC

Pro 160 acc Thr gt t Val1 a tg Met ttC Phe ct C Leu 240 9CC Ala atc Ile t cg Se r ctc Le u Ct t Le u atc Ile

C

Arg aca Thr acc Th r 145 aaa Lys ca a Gin ttc Phe a gc Se r a CC Thr 225 Ca C His aca Thr agc Ser ttg Leu agt Ser 305 ttt Phe ga C Asp gaa Giu Ca g Gin 130 aac As n ct t Le u ttc Phe ctC Le u cac His 210 gCC Ala ctc Leu gg C Gly tgc Cys tgc Cys 290 act Thr CCt Pro gtc acc Val .Thr 100 CCC 99C Pro Gly 115 gtC taC Val Tyr ttC atC Phe Ile Cta gca Leu Ala CgC gat Arg Asp 180 tcc tca Ser Ser 195 acc gaC Thr Asp ttC atc Phe Ile ggC acc Gly Thr gtC caa Val Gin 260 gCt gat Ala Asp 275 gCg aag Ala Lys qtg Ctg Val Leu CCg act Pro Thr t CC Ser gCC Ala aac As n CCa Pro agc Ser 165 aca Thr ga C Asp Ct C Le u aag Ly s

CCC

Pro 245 gt C Val1 Ca g Gin CCg Pro gtt Val1 ggg Gly 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 WO 01/00805 310 315 320 acg aag att tcc gca aag ttt tcg acg gct cca ccc cag Thr Lys Ile Ser Ala Lys Phe Ser Thr Ala Pro Pro Gin 330 335 ctt cag aat cca tct aga tct cca aga cct ccg caa cag Leu Gin Asn Pro Ser Arg Ser Pro Arg Pro Pro Gin Gin 345 350 ccg cac ccg aag gcc acg aga acc tct tca tcc tgg tcc Pro His Pro Lys Ala Thr Arg Thr Ser Ser Ser Trp Ser 360 365 370 ccg atg tct cca ttg gtc acg ggt ccg ctt acg gag aag Pro Met Ser Pro Leu Val Thr Giy Pro Leu Thr Giu Lys 375 380 385 tgq tgq gcc gga tcg caa tagcagcagt ggctcaaatt ggg Trp Trp Ala Gly Ser Gin 390 395 <210> 242 <211> 395 <212> PRT <213> Corynebacterium glutamicum aat As n att Ile 355 cag Gin aat Asn t cc Ser 340 ccg Pro tac Tyr ctg Leu PCTIBOOIOO926 325 cgg 1123 Arg atg 1171 Met ccg 1219 Pro aca 1267 Thr 1308 <400> 242 Met Thr Lys Ala Vai Val Ile Giy Giy 1 Th r Asn Arg His Val Ile Arg Th r Th r 145 Lys Le u Le u Th r Lys Giu Th r 100 Giy Tyr Ile Ala Giy Aila T rp Thr Arg Asp Lys 120 Ile Se r Th r Tyr Gly Tyr Se r Val Aia 105 Le u Asp Pro Se r Gly 10 Giu Th r Le u Ile Ile Met Arg Gly Giy Le u 170 Leu Vali Phe Met Asp 75 Asp Aia Thr Phe Met 155 L ys Ala Glu Giy Met Asp Giu Gi u Se r Asn Leu Asn 175 Thr Gin Phe Se r Leu Phe Ser Aia Phe Pro 160 Thr WO 01/00805 WO 0100805PCTIiBOO/00926 Gin Phe Arq Asp Thr Lys Leu Arg Gin Ile Leu Ser Tyr Pro Ala Val 180 185 190 Phe Leu Ser Ser Asp Pro Ser His Thr Pro Ala Leu Tyr His Leu Met 195 200 .205 Ser His Thr Asp Leu Val Gin Gly Val Ser Tyr Pro Arg Gly Gly Phe 210 215 220 Thr Ala Phe Ile Lys Ala Leu Ile Ser Leu Ile Asp Asp Ala Val Leu 225 230 235 240 His Leu Gly Thr Pro Val Ser Ala Ile Thr Thr Gin Gly Arg Asn Ala 245 250 255 Thr Gly Val Gin Val Gly Ser Glu Phe Ile Giu Ala Asp Ile Val Ile 260 265 270 Ser Cys Ala Asp Gin His His Thr Giu Thr Gin Leu Leu Pro Ala Ser 275 280 285 Leu Cys Ala Lys Pro Glu Thr Ser Trp Lys Asn Lys Gin Pro Gly Leu 290 295 300 Ser Thr Val Leu Val Leu Ala Gly Val Lys Gly Giu His Thr Leu Leu 305 310 315 320 Phe Pro Pro Thr Gly Thr Lys Ile Ser Ala Lys Phe Ser Thr Ala Pro 325 330 335 Pro Gin Asn Ser Arg Leu Gin Asn Pro Ser Arg Ser Pro Arg Pro Pro 340 345 350 Gin Gin Ile Pro Met Pro His Pro Lys Ala Thr Arg Thr Ser Ser Ser 355 360 365 Trp Ser Gin Tyr Pro Pro Met Ser Pro Leu Val Thr Gly Pro Leu Thr 370 375 380 Giu Lys Asn Leu Thr Trp Trp Ala Gly Ser Gin 385 390 395 <210> 243 <211> 450 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(427) <223> RXA01293 <400> 243 cqatgccgca cccgaaggcc acgagaacct cttcatcctg gtcccagtac ccgccgatgt ctccattggt cacgqgtccg cttacggaga agaatctgac atg gtg ggc cqg atc 115 Met Val Gly Arg Ile 1 gca Ala ttg Le u gca Al a acc Thr gt a ValI gt a Val1 gg g Gly WO 01/00805 ata gca g.

Ile Ala A.

gaa agc ci Glu Ser A; gac cga t~ Asp Arg T' ctg gca. c~ Leu Ala G gat ggc ct Asp Gly LE tct atg tc Ser Met C1 gac aac ac Asp Asn Se ca la rg In

'S

gtg Val1 att Ile aac Asn t cg Ser tat Tyr ttq Leu gt g ValI gct Al a gtt Val1 t C Ser gcg Ala ga C Asp 75 atc Ile ga t Asp ca a Gin gtg Val1 t gg T rp ttc Phe 60 gca Ala tct Ser c9g Arg att Ile cag Gin a gc Ser 45 tt Phe ggt Gly gca Ala act Thr ggg cga tgg gct ggc att gat Gly Arg Trp Ala Gly Ile Asp 15 cgc acc atc ggc cct gcc gac Arg Thr Ile Gly Pro Ala Asp 30 ggc ggg tcc att ggc ccc tca Gly Gly Ser Ile Gly Pro Ser agg qqt tct aac aaa tcc cgc Arg Gly Ser Asn Lys Ser Arg gac acc aca gtt cca ggg gtg Asp Thr Thr Val Pro Gly Val 80 gaa aac gtg ctc aag cgt ctc Glu Asn Val Leu Lys Arg Leu 95 100 taacqqgact tttttctttt gct PCTIBOOIOO926 ggt 163 Gly ttc 211 Phe cac 259 His aaa 307 Lys gqc 355 Gly cgc 403 Arg 450 <210> 244 <211> 109 <212> PRT <213> Corynebacterium glutamicum <400> 244 Met Val Gly Arg Ile Ala Ile Ala Ala 1 5 Ala Gly Ile Asp Gly Leu Glu Ser Arg Gly Pro Ala Asp Phe Ala Asp Arg Tyr Ile Gly Pro Ser His Thr Leu Ala Gin Asn Lys Ser Arg Lys Val Asp Gly Leu 70 Val Pro Gly Val Gly Val Ser Met Cys Leu Lys Arg Leu Arg Gly Asp Asn Ser 100 105 <210> 245 <211> 1386 <212> DNA <213> Corynebacterium glutamicum Val1 10 Ile Asn Se r Tyr Leu 90 Val Arg TIhr Gly Gly Thr Asn T rp.

Ile Se r Se r Thr Val1 WO 01/00805 <220> <221> CDS <222> (1363) <223> RXA02310 <400> 245 gaaatggaca aaaagctttt tgtttaaccg tagtcgacgg PCTJIBOO/00926 aaggcacttg aagcaccttg ggttagtctt cttgctctcc ggactgagac aatggggttt gtg tct aca act ttt Val Ser Thr Thr Phe gat gtg ttg atc atc Asp Val Leu Ile Ile cat gcg qcc agg act His Ala Ala Arg Thr ttc ccg cgg gat aaa Phe Pro Arg Asp Lys cac cag cta gaa ctt His Gin Leu Giu Leu ttc aac aag ggc ttg Phe Asn Lys Gly Leu ccg tgg ccg gag aca Pro Trp Pro Giu Thr atg gag ttc gat gat Met Giu Phe Asp Asp 105 gta acc acg tgg gag Vai Thr Thr Trp Giu 120 aat ttc ttg gaa qgc Asn Phe Leu Glu Giy 135 gtc aag gcg aag cat Val Lys Ala Lys His 150 ggt aag aaa ctg ggt Giy Lys Lys Leu Giy 170 gcg gct cgt gct tat Ala Ala Arg Ala Tyr 185 g cg Ala ctt Leu t gt Cys ggt Gly ctg Le u ttc Ph e tta Leu g cg Ala gt g Val1 140 att Ile cag Gin ggc Giy ca a Gin Gi y gt t Val1 cat His acg Thr ttc Phe agc Ser 125 att Ile att Ile t gg Trp ccc Pro aca Th r qa t Asp gct Ala 9gt Gly aat Asn cgc Arg 110 gcc Ala aat Asn gcc Ala caa Gin qcc Ala tcc Ser gc9 Ala ga t Asp gag Giu t cg Se r 100 gag Giu a gg Arg aaa Lys cct Pro 99c Gly 180 gtc Val1 tcc Se r att Ile tat Ty r gcg Al a cgg Arg ga a Giu qgg Gly acc Th r ttc Phe 165 att Ile 115 163 211 259 307 355 403 451 499 547 595 643 tgt gaa act ccg ctg tct gat gaa ccg tgg att Cys Glu Thr Pro Leu Ser Asp Giu Pro Trp Ile WO 01/00805 WO 0100805PCTIEiBOOIOO926 cac tcc cat His Ser tat ggg Tyr Gly 215 ggc gcg Gly Ala 230 ttg tig Leu Leu ccc gag Pro Glu tcg aat Ser Asn tgt gtg Cys Val 295 gcq gcg Ala Ala 310 acc ttg Thr Leu atg ttg Met Leu ccg atg Pro Met atg ccg Met Pro 375 aaa gac Lys Asp 390 tgg gcg Trp Ala His 200 tgg Trp ctc Le u agc Se r cac His gtg ValI 280 aac As n atg Met gt a Val1 9 Cg Al a gct Ala 360 gcg Ala ctg Leu Cgg gtg Val att Ile t cg Se r ttc Phe gac Asp 265 gct Al a ccg Pro gcc Al a t gg T rp cgc Arg 345 ggg Gly gct Ala ctc Leu aa g gaa ctg cgc gat gaa gat ggt gtg gtg cag cca gga Leu ccg Pro ga t Asp 235 gcg Al a gcc Al a gcg Ala aac As n ga c Asp 315 cat His qc t Ala ctc Le u cq t Arg a gg Arg 395 tcC Glu aac Asn cca Pro cgt Arg ct g Leu 270 atg Met 9gc Gly gt g Val1 cgc Arg ctg Leu 350 cg c Arg gg c Gly ca g Gln t gg Trp Asp ggc GI y g cg Ala cgt Ar g 255 ctg Leu ct g Leu atc Ile ga a Glu ga c Asp 335 ctg Le u g gg Gl y aac Asn gcc Ala gac Asp Gly acg Th r aag Lys 240 aaa L ys cct Pro atc Ile gac Asp aa c Asn 320 gcg Al a acg Thr ccg Pro ct g Le u gcc Al a 400 tcg Ser Val1 gtg Val1 225 atc I le gca Ala atg Met gg c Gly tat Tyr 305 ccc Pro tac Tyr tac Tyr ct g Le u atc Ile 385 gga Gly act Thr 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1386 Arg Lys Gly Ser Pro 410 taatcgccga gtgcagcgcg acg <210> 246 <211> 421 WO 01/00805 <212> PRT <213> Corynebacterium glutamicum PCT/IBOO/00926 <400> 246 Val Ser Thr Thr Phe Asp Val 1 Al a Ile Thr Val1 Giy Ser Ly s Pro Gly 145 Val1 Glu Asp Val Val1 225 Ilie Ala Met Se r Asp Pro Thr Ser Ala Ser Ile 130 Gin Arg Val Glu Val 210 Asn As n Trp Gly His Phe His Phe 70 Pro Met Val Asn Val 150 Gly Ala His Tyr Gi y 230 Le u Pro Ser Ala Pro Gin 55 Asn Trp Giu Th r Phe 135 Lys Lys Ala Se r Giy 215 Ala Le u Glu As n Leu Ile Ile Gly 10 Ala Arg Thr Gly Arg Asp Lys Thr Leu Giu Leu Leu Lys Giy Leu Lys 75 Pro Giu Thr Tyr Phe Asp Asp Leu 105 Thr Trp Glu Asn 120 Leu Glu Gly Val Ala Lys His Val 155 Lys Leu Giy Arg 170 Arg Ala Tyr Cys 185 His Val Glu Leu 200 Trp Ile Phe Pro Leu Ser Thr Asp 235 Ser Phe Tyr Ala 250 His Asp Val Ala 265 Val Ala Gly Ala 280 Ala Leu Cys Gly Le u Phe Leu Al a Val1 140 Ile Gin Giu A rgc Leu 220 Thr Gly Ser Asn Gly Gin Gly Val His Thr Phe Se r 125 Ile Ile Trp Th r Asp 205 Gi y Arg Gin Al a Trp 285 Pro Thr Asp Ala Gly As n Arg 110 Ala As n Al a Gin Pro 190 Glu Asn Pro Arg Leu 270 Met Ser Leu Gi y Asp Phe Lys Leu Glin His Asp Arg 175 Le u Asp Gly Ala Arg 255 Le u Leu Gly Le u Le u Gin Gly Gly Ala Asp Al a Gly 160 Asp Se r Gly Th r Lys 240 Lys Pro Ile Gly Asp 290 Ser Ala Ala Cys Val Asn Pro Leu Asn Gly Giu Gly Ile Asp 295 300 Tyr 305 P rc Tyr Tyr Le u Ile 385 Gly Thr WO 01/00805 *Gly Leu G ILys Arg A.

*Gly Giu Ti Pro Gin P1 355 Gin Lys AJ 370 Thr Giu GI Ser Ala Il Ser Ser Le iu sp hr la Lu Thr Leu 325 Phe Leu Ile Asp Se r 405 ValI Ala 310 Th r Met Pro Met Lys 390 Trp Aila Leu Leu Met Pro 375 Asp Ala Ala T rp Ar g 345 Gly Ala Leu Lys Vali Pro 330 Thr Pro Ala Ala Gly 410 Asp 315 His Ala Leu Arg Arg 395 Ser Glu Asp 335 Leu Gly Asn Ala Asp 415 PCT/IB00/00926 Asn 320 Ala Thr Pro Le u Ala 400 Ser <210> 247 <211> 1170 <212> DNA <213> Corynebacteriui giutarnicum <220> <221> COS <222> (101)..(1147) <223> RXA02718 <400> 247 tccaccaaaa ttcagcccgc atccccctat tcgattgaag cgatgcccat gaccttgacc tcgacaaatt tccggaggtc ttc Phe aca Th r cga Arg cat His agt Ser ct C Leu ga t Asp atc Ile aag Ly s ctc Le u ca a Gin gcc Ala ctt Leu tct Se r atc Ile gag Glu cat His tat Tyr 45 g aa Giu cag Gin cta Le u ctt Leu 30 g cg Al a aaa Lys gct Ala aca Th r cgc Arg t gg T rp ctt Leu tgt Cys att Ile agt Se r gct Ala gag Giu gcc Aila gacqtctcct tgagcagttt gtq cqa. gat cgt ttg Val Arq Asp Arg Leu 1 gct gat atc ggc gct Ala Asp Ile Gly Ala ttc gtg ctc aat gga Phe Val Leu Asn Gly ggt ttc ctg gcg qcg Giy Phe Leu Ala Ala tcc gtc Ctt gac gcc.

Ser Val Leu Asp Ala ttg att cat gac gat Leu Ile His Asp Asp WO 01/00805 WO 0100805PCT11B00100926 att Ile gct Al a cac His t gg Trp gcc Ala 150 9gc Gly gag Glu gcg Al a ctt Le u ca g Gin 230 gg c Gly ctt Leu a cg Th r gct Ala gag Glu 310 atc Ile gtg Val ttt Phe gca Ala 135 cgc Arg cag Gin ctt Leu cgc Arg atc Ile 215 ttg Leu aaa Ly s g cg Ala gcc Ala gtc Vali 295 cag Gin gat Asp gaa Glu ggc Gly 120 gaa Giu acg Thr ctg Le u g Cg Ala cca Pro 200 ga c Asp agg Arg cc a Pro ctc Le u att Ile 280 att Ile cg a Arg t ct Se r gct Al a 105 gtt Val1 gac Asp agg Arg ct t Le u ga t Asp 185 ttg Le u gcg Ala gat Asp gct Ala gct Al a 265 cgc Arg aca Thr att Ile tct Se r gat Asp t ca Ser atg Met gat Asp gat Asp 170 tct Se r cac His ct C Leu gat Asp gga Gly 250 cta Leu gca Al a gag Glu tcc Se r ga t Asp ca C His gtc Val1 ct g Le u gct Ala 155 att Ile gt C ValI ct g Le u ctc Le u ct g Leu 235 ga c Asp caa Gin ggt Gly cat His ca g Gin 315 a cc Thr cg c Arg t cg Ser cag Gin 140 t gg Trp tat Tyr aac As n ggc Gly cac His 220 ct t Le u gat Asp cgc Arg gtt Val1 att Ile 300 ctg Le u cg g Arg gcc Ala att Ile 125 ga t Asp cgt Arg ct a Leu cg c Arg gcc Ala 205 tac Tyr ggt Gi y atc Ile g ct Ala gga Gly 285 cga Arg act Th r cgc Arg aat As n 110 ttg Leu tcc Se r gg c Gly gag Glu ttc Ph e 190 tcc Se r ggc Gly gt g Val cgt Arg gat Asp 270 aag Lys gct Al a ga a Glu gga Gly 95 aat As n gct Al a g gt Gly atg Met t cg Se r 175 a aa Lys att Ile cac His ttt Phe gaa Glu 255 aag Lys gtq Val1 act Thr tcc Ser gcc Al a ttc Phe ggc Gly ttg Le u cg t Arg 160 cac His acg Th r gct Al a ga c Asp gg g Gly 240 ggc Gly caa Gin act Th r gg t Gi y gg t Gly 320 gtt Val1 ga t Asp 115 gca Al a gag Giu gt t Val1 gag Glu tac Tyr 195 t cg Se r att Ile gc t Al a act Th r ga a Glu 275 gaa Giu ga a Gi u ca c His ca c His 100 ccc Pro ttg Leu gca Al a att Ile t cg Se r 180 a cg Th r ccg Pro gca Al a atc Ile gt t Val1 260 gct Ala gat Asp gaa Glu ct c Leu cgg Arg gag Glu gt g ValI ttg Le u ggc Gly 165 gtg Val att Ile cag Gin ttc Phe acc Th r 245 ctt Le u gca Ala att Ile gtt Val1 gat Asp 325 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 WO 01/00805 gat gta gac atc Asp Val Asp Ile atc cgc tca acc Ile Arg Ser Thr 345 PCTJIBOO/00926 ccc gat gag gtg cgc gca cag ttg cqg gca ctg gct 1123 Pro Asp Giu Val Arg Ala Gin Leu Arg Ala Leu Ala 330 335 340 gaa cgt cgg atg tagtagacgc gtatgacact ttt 1170 Giu Arg Arg Met <210> 248 <211> 349 <212> PRT <213> Corynebacterium giutamicum <400> 248 Vai Arg Asp Arg Leu Thr Gin Phe Leu 1 Ala Phe Gly Se r Leu Pro Giu Asp Se r 145 Th r Ala Ala Giy Ile 225 Asp Asp Val1 Phe ValI Ile Th r Gly Met 130 Ala Glu Asn Al a Gly 210 Gi y Pro Ile Le u Leu Leu His ValI Asp 115 Ala Giu Val Giu T yr 195 Se r Ile Ala Gly Asn Ala Asp Asp His 100 Pro Leu Ala Ile Se r 180 Thr Pro Al a Ile 5 Al a Gly Ala Al a Asp Arg Giu Val1 Le u Gly 165 Val Ile Gin Phe Thr Pro Gly Gin Ala 70 Ile Al a His Trp Ala 150 Gly Giu Ala Le u Gin 230 Giy Val1 Lys Gly 55 Ala Ile ValI Phe Ala 135 Arg Gin Le u Arg Ilie 215 Le u Lys Th r Arq 40 His Se r Asp Giu Gly 120 Glu Thr Le u Ala Pro 200 Asp Ar g Pro Asp 25 Ile Lys Le u Ser Al a 105 Val1 Asp Arg Leu Asp 185 Le u Ala Asp Ala Asp 10 Ala Arg Asn Giu Se r 90 Asp Ser Met Asp Asp 170 Se r His Le u Asp Gly Ala Gin Val Aia Pro Leu Ser Ser Phe Ile 75 Asp Thr His Arg Val Ser Leu Gin 140 Ala Trp 155 Ile Tyr Vai Asn Leu Giy Leu His 220 Leu Leu 235 Asp Asp Giu His Tyr Gi u Gin Arg Ala Ile 125 Asp Arg Le iu Arg Ala 205 Tyr Gly Ile Le u Le u Aila Lys Ala Arg Asn 110 Le u Se r Giy Giu Phe 190 Se r Gly Val Arg Thr Arg T rp Leu Cys Gly Asn Aila Gly Met Ser 175 Lys Ile His Phe Glu Ile Ser Ala Gi u Ala Al a Phe Gi y Le u Arg 160 His Th r Al a Asp Giy 240 Gly WO 01/00805 WO 0100805PCTFBOO/00926 Ly s Se r Se r Al a 305 Le u Le u Arg Pro Pro 290 Glu Ala Arg Thr Gl u 275 Glu Gi u His Ala Ala 265 Arg Thr Ile Ile Thr 345 250 Le u Al a Glu Ser Pro 330 Glu <210> 249 <211> 852 (212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(829) <223> RXA01067 <400> 249 ggctttaagg ccgaggtgcc tggctgaact gcagcccctc aatggcaaac acaaatagtt tgcatggcag aatagcttag gta ValI atg Met gaa Glu t gc Cys gaa Glu aac As n ccc Pro ga t Asp gg C Gly att Ile aa c Asn cg a Arg atc Ile aat Asn aa g Lys ctt Le u cg t Arg gtg Val cc t Pro gga Gly cgt Arg ggt Gly cgt Arg ct g Le u qc C Ala cgc Arg ggc Gly gtt Val1 tcC Ser 75 cga Arg ca a Gln tgg Trp gag Glu ccg Pro 60 acc Th r cga Arg ttc Phe g ct Al a gca Ala tac T yr ga t Asp caa Gin cagaccctga gatcaggcta gtg agt gaa ttc caa Val Ser Glu Phe Gln 1 cat att gcg ctt gtc His Ile Ala Leu Val ggc atg aag cgc acc Gly Met Lys Arg Thr gat gtt gtt gat gca Asp Val Val Asp Ala tat gcc ttc'tct act Tyr Ala Phe Ser Thr ttc ctc atg gga ttc Phe Leu Met Gly Phe cta cat gaa aag ggc Leu His Glu Lys Gly 100 115 163 211 259 307 355 403 WO 01/00805 WO 0100805PCTIBOO/00926 gt Val atc Ile atg Met ga c Asp 150 cc9 Pro gac Asp acg Thr caa Gin gtc tgg git ggc Trp Val Gly cgt cgt ccc cgc ctg tgq cgt tcg Arg Arg Pro Arg Leu Trp Arg Ser 110 115 ga a Gi u 125 aa c Asn cgc Arq acc Thr ttc Phe ca 9 Gin 205 tc Phe a cc Thr atc Ile ctg Le u gaa Glu 180 aag Lys gi c Val ga c Asp acc Th r atc Ile c gc Arg 165 cct Pro cgc Arg tac Tyr gcg Ala gat cgc aga tic gga agc gca Vai Leu Giu Tyr Ala Lys Arg Asp.Arg Arg Phe Giy Ser Ala 230 235 240 taatgcccac caaccagccg acg <210> 250 <211> 243 <212> PRT <213> Corynebacterium giutamicum <400> 250 Val Ser Giu Phe Gin Vai Pro Giu Ile Pro Aia 1 5 10 His Ile Ala Leu Val Met Asp Gly Asn Gly Arg 25 Gly Met Lys Arg Thr Giu Giy His Lys Arg Gly 40 Asp Val Val Asp Ala Cys Ile Giu Leu Gly Val 55 Tyr Ala Phe Ser Thr Glu Asn Trp Arg Arg Ser 70 75 Gin Phe Leu Pro Lys Trp Ala Thr Giu Arg Giu Ala Val Leu Leu Pro Tyr Leu Ser Ala Thr Asp Giu Vai Arg WO 01/00805 WO 0100805PCT/IBOO/00926 Phe Leu Met Gly Phe Asfl Arq Asp Val Leu Arg Arg Gin Leu His Leu Trp Lys Asp 130 Arg Ala 145 Giu Gly Phe Leu Ser Gly Ala Glu 210 Asp Leu 225.

Gly Ser Glu Arg 115 Asn Glu Ly s Asp Glu 195 Met T yr Ala Lys 100 Ser Thr Ile Le u Glu 180 Lys Val1 Asp Gly Val1 Thr Ile Arg 165 Pro Arq Tyr Ala Gly Al a Val1 140 Al a Lys Le u Trp Asp 220 Arg Arg Glu 125 Asn Arg Thr P he Gin 205 Phe Asp <210> 251 <211> 564 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101) .(541) <223> RXA01269 <400> 251 gattatctct gcgccgattc agctggcaat agcagcagtt tccgattcta tttcgtcaac cacgacctgg gaaagacggt att aag ttt cga acc atg ctt gaa cca Ile Lys Phe Arg Thr Met Leu Glu Pro gaa cag cgt cta act aaa gtt gga aag Giu Gin Arg Leu Thr Lys Vai Gly Lys 30 gat gag tta cct aca ctc tgg aat gta Asp Giu Leu Pro Thr Leu Trp Asn Vai gat gaa Asp Glu 15 ctt ctg Leu Leu ttt aaa Phe Lys gtattgagag ctcatggtcg gtg gta ttt qag atg 115 Vai Val Phe Glu Met 1 aaa cat gta act gat 163 Lys His Vai Thr Asp cgg gaa acg agt tia 211 Arg Giu Thr Ser Leu ggt gat atg agc ctt 259 Gly Asp Met Ser Leu WO 01/00805 45 gta ggg cct cga cct ttg ctt gtt agc Val Gly Pro Arg Pro Leu Leo Val Ser gaa caa gct cga cgc cat gaa gtt. cgt Glu Gin Ala Arg Arg His Glu Val Arg cag gtg aat ggc cgt aat caa act act Gin Val Asn Gly Arg Asn Gin Thr Thr gat gtc gaa tat gtg gat cgc tgt agt Asp Val Glu Tyr Val Asp Arg Cys Ser 105 110 tta atc gcc act gta aaa aca gtt ctt Leu Ile Ala Thr Val Lys Thr Val Leu 120 125 gaa ggt cat gtc acg atg cca tcc ttc Glu Gly His Val Thr Met Pro Ser Phe 135 140 tagcaggtaa aaattttact ttc <210> 252 <211> 147 <212> PRT <213> Corynebacterium giutamicum <400> 252 Val Val Phe Glu Met Ile Lys Phe Arg 1 Lys His Val Thr Asp Glu Gin Arg Leu Arg Glu Thr Ser Leu Asp Glu Leu Pro Gly Asp Met Ser Leu Val Gly Pro Arg 55 Glu His Tyr Ser Ser Giu Gin Ala Arg 70 Ile Thr Giy Leo Ala Gin Val Asn Gly Giu Arg Leo Lys Leo Asp Val Glu Tyr 100 105 Leo Asp Phe Lys Ile Le Ile Ala Thr 115 120 Lys Gly Ile Ser Asn Glu Giy His Val 130 135 PCT/IBOO/00926 cat His act Thr cg a Arg gat Asp gg c Giy 130 aga Arg tac Tyr ggt Giy ctt Leu ttc Phe 115 att Ile aaa Lys tct Ser ttg Leo aa g Lys 100 aaa Lys agt Ser Leo Vai Trp Leo Glu Gin Arg Thr Pro 140 Glu Leo Lys Leo Gly Asp Ly s Lys Glu WO 01/00805 WO 0100805PCTIIBO0/00926 Glu Arg Lys 145 <210> 253 <211> 554 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> <223> RXA01205 <400> 253 qta tcg 91 Val Ser A.

1 tgc gcg g( Cys Ala G.

qgc gat ti Gly Asp S4 acc tca 9' Thr Ser A.

ttt atc 9' Phe Ile A.

atc cca c' Ile Pro Li gga gca tv Gly Ala Si ct9 tcc a' Leu Ser I 1 tgg gcg a, Trp Ala S, 130 cca ctg t Pro Leu P1 145 gct gtc a, tat Tyr gt t Val1 gg c Gly t ca Ser t tg Leu ctc Le u ccc Pro 100 gga Gly gc c Al a gcc Ala gcc ccg Pro 5 tt g Leu tcc Se r gga Gly atc Ile ga c Asp ttt Phe cac His gtg Val1 acc Thr g t9 Val ccg gcc atc Pro Ala Ile ccc cat aat Pro His Asn atq ctc atc Met Leu Ile aaa atc aac Lys Ile Asn 55 tca ccc atc Ser Pro Ile 70 ctc gtg atg Leu Val Met tcc ccg gac Ser Pro Asp acc cat agg Thr His Arg 120 gca ttc ggc Ala Phe Gly 135 gga tcg agc Gly Ser Ser 150 cca gtg atg Pro Val Met g ca Ala 10 gaa Glu ctg Le u agc Se r qtt ValI gtg Val1 90 atg Met gtg Val1 9tg Val tgt Cys agc Ser 170 gcg Ala ccc Pro ctg Leu ctg Leu gtt Val1 75 gtt Val1 cat His gtc Val1 agc Ser ggc Gly 155 c9g Arg gct Ala t cg Se r ttg Leu tat Tyr ct c Leu a gg Arg ctg Leu cta Leu ttc Phe 140 atc Ile cga Arg ct c Le u cga Arg gct Ala 99 c Gly gcc Al a cgc Arg cac His gtg Val1 125 t cc Se r ctc Le u gcc Ala gta Val1 ata Ile 9 ca Ala qca Ala 9cc Ala gtg Val1 ca c His 110 ctc Leu gt c Val1 atc Ile 9cc Ala gga Gly ttt Phe gca Ala gct Ala gtg Val1 Gly cqa Arg tac Tyr gt t Val1 qc C Ala aaa Lys 175 att Ile atg Met t cg Se r ga t Asp 9cc Al a agg Arq ct g Le u acc Th r ccg Pro gtc Val1 160 ctt Leu 48 96 144 192 240 288 336 384 432 480 528 Ala Val Thr Ala gat taagtgattq tcactttgga ttg WO 01/00805 WO 0100805PCTIBOOIOO926 Asp <210> 254 <211> 177 <212> PRT <213> Corynebacterium glutamicum <400> 254 Val Ser Ala Tyr Pro Pro Ala Ile Ile 1 Cys Gly Thr Phe Ile Gly Leu Trp Pro 145 Ala Asp Pro Met Lys Ser 70 Leu Ser Th r Ala Gly 150 Pro His Leu Ile Pro Val1 Pro His Phe 135 Se r Val Asn Ile Asn Ile Met Asp Arg 120 GI y Ser Met Al a 10 Glu Leu Se r Val1 ValI Met Val1 Val C ys Se r 170 Gly Phe Ala Ala Val1 Gly Arg T yr Val Ala Lys 175 Ile Met Ser Asp Ala Arg Le u Th r Pro Val 160 Leu <210> 255 <211> 882 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(859) <223> RXA01576 <400> 255 atcggtgatc tttcgcgatt gatacgtgcg cgattgcgtg cccgccaqct gctcaagatc aatggatttc atgacaatca agttatcatc actggggtcg qtg gca cct aac agg 115 WO 01/00805 WO 0100805PCTJIBOO/00926 Val Ala Pro Asn Arg aaa Lys aa a Lys gag Giu cct Pro ga a Giu gga Gly ccc Pro gt g Val ga c Asp cay Gin 150 tca Se r cgc Arg gyc Gly t gg Trp atg Met atc Ile aac Asn gca Ala aa t Asn atc Ile caa Gin ggt Gly aaa Lys att Ilie 135 gtg Val1 ccg Pro gta Val1 atg Met cgc Arg 215 agc Se r a gc Ser agc Ser aca Th r att Ilie cat His gg t Gly gca Ala gac Asp 120 atc Ile cca Pro aaa Lys ttc Phe t cg Se r 200 att Ile a ag Lys cat His ga c Asp qtt Val1 yt t Val gcg Ala gcc Ala aaa Lys 105 gt t Val1 gt c ValI ct a Le u acc Th r aac As n 185 cat His t ca Ser gg g Gly cat His ttt Ph e atc Ile gcg Al a gcc Ala g cg Ala tac Tyr atc Ile qgc GI y atc Ile cy c Arg 170 caa Gin gca Ala ga a Giu caa Gin g ca Ala aaa Lys cgg Arg gtC Val gyc Gly 75 atc Ile ttt Phe cgc Ar g a cg Thr aag Lys 155 cga Arg aaa Lys tcc Ser yaa Giu tcc Se r cta Leu ga c Asp gaa Glu aac As n 60 g ca Ala caa Gin gta Val1 aty Met cgt Ar y 140 cg c Ary ctt Le u gtg Val gaa Giu ccc Pro 220 ct g Le u 99c Gly acc Thr gtt Val 45 gat Asp cac His acc Thr act Th r gty Val1 125 ttt Phe ct t Le u 9yg Gly gcg Ala atc Ile 205 gt a Val1 ctc Le u tct Se r t gg Trp 30 tta Le u yg C Gly ct g Le u gg c Gi y ttc Phe 110 ga Giu gga Gly gtg Val1 ctc Leu cay Gin 190 gtC Val1 gac Asp aac Asn att Ile cta Leu gag Giu tcc Ser gtc Val1 atc Ile ga c Asp cy a Arg cg c Arg ct y Le u acc Thr 175 ga a Giu gac Asp atc Ile gy c Gly cct Pro 9t C Val1 aa c Asn ccg Pro aac Asn 80 gaa Glu gcc Ala ctg Leu cca Pro cgc Arg 160 gac Asp atg Met caa Gin ctc Le u gt a Val1 1 atc Ile gt a Val1 gca Ala ga c Asp cac His tac Tyr ga c Asp cy t Arg cgc Arg 145 acc Thr gcc Ala aac Asn atc Ile tac Tyr 225 aac Asn atg Met cct Pro ct c Leu aac Asn ccc Pro ycc Ala ggC Gly gcc Ala 130 ca a Gin gt g Val cac His atc Ile gat Asp 210 a cc Th r atc Ile gat Asp tgt Cys aaa Lys tcc Se r gtg Val1 cy c Arg caa Gin 115 gag Glu gcc Ala gtc Val1 aac As n cgc Ary 195 ga a Giu ga a Giu ct y Leu tca Ser aac Asn ttc Phe yaa Glu ctc Leu caa Gin ca a Gin yt g Val ga c Asp ct 9 Le u 165 ct g Leu aac As n gyc Gly tcc Se r gac Asp 163 211 259 307 355 403 451 499 547 595 643 691 739 787 835 WO 01/00805 230 235 240 ggc ttc cta gcg agg aga ctc cca tgacgcagac caccacccaa atc Gly Phe Leu Ala Arg Arg Leu Pro 250 <210> 256 <211> 253 <212> PRT <213> Corynebacterium giutarnicum PCTIBOO/00926 245 882 <400> 256 Val Ala Pro Asn Arg Lys Ile Ser His 1 Ile Val1 Ala Asp His T yr Asp Arg Arg 145 Thr Al a Asn Ile Tyr 225 Asn Met Pro Le u Asn Pro Ala Gly Al a 130 Gin Val1 His Ile Asp 210 T hr Ile Asp Cys Lys Se r Val1 Arg Gin 115 Glu Ala Val As n Arg 195 Glu Glu As n Al a Asn Ile Gin Gly Ly s Ile 135 Val1 Pro Val1 Met Ar g 215 Se r Phe Ser Thr 40 Ile His Gly Al a Asp 120 Ile Pro Ly s Phe Se r 200 Ile Lys Leu Asp Val1 Val1 Ala Ala Lys 105 Val1 Val1 Le u Thr Asn 185 His Se r Gi y Ala His 10 Ph e Ile Al a Ala Al a Tyr Ile Gly Ile Arg 170 Gin Ala Glu Gln Arg 250 Gi y Th r Val1 Asp His Th r Th r Val1 125 Phe Leu Gly Ala Ile 205 Val1 Le u Pro Ile Le u Giu Se r Val1 Ile Asp Arq Arg Le u Thr 175 Giu Asp Ile Gly Pro Val1 Asn Pro Asn Glu Ala Le u Pro Arg 160 As p Met Gin Le u Val1 240 Leu Ala Asp 245 WO 01/00805 <210> 257 <211> 1173 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1150) <223> RXN02309 <400> 257 gtgccttccg tcgactacgg ttaaacaaaa agctttttgt aaagaatgaa tccacactcg atcaccaaag gtagcgatga PCT/LBOO/00926 ccaittcact ggattcaccg atg agt agc ggc cga Met Ser Ser Gly Arg acc Thr aca Thr atc Ile t cg Se r gcc Al a ttc Ph e gt a Val1 gag Glu aac As n gg t Gly 150 aca Thr acc Thr tct Ser gcc Ala gaa Glu aaa Lys aaa Lys acc Thr 105 atg Met gc c Ala agt Se r ga a Glu cg i Ar g ca g Gin atg Met ga c Asp cga Arg cca Pro cac His cgc Arg at c Ile ca 9 Gin cta Le u 170 tcc Se r gtc Val1 gig Val ttc Phe ttc Phe 75 ctc Le u ct g Le u cg c Arg ctc Leu ctg Le u 155 gt c Val1 cac His gag Giu cag Gin ci c Leu cqc Arg icc Se r gcc Ala ggc Gly gca Ala 140 ggt Giy acc Th r ggg Gi y ttt Phe gia Val1 gt C Val1 ccc Pro gaa Glu acc Thr gic Val1 125 ggc Gly acc Th r ggc Gly aaa Lys ccc Pro ctc Leu gtC Val1 gca Aia 80 atc Ile cac His gct Ala ctc Leu gtc Val1 160 cg C Arg 1 g aa Giu gag Giu ctg Leu a ig Met ctg Leu aaa Lys gac Asp aa C Asn cta Leu 145 gcc Aia ga a Glu gg t Gly cia Le u cac His cac His ctg Leu gc C Al a gat Asp gcg Ala 130 gca Ala cac His aca Th r gia Val1 a cg Thr act Th r cta Le u gcc Aia gcc Ala gt c Val 115 cg a Ar g cat His tt Phe gtC ValI icc Se r gcc Al a gaa Glu aca Thr icc Se r gt Val 100 atg Met i gg Trp gca Ala gcc Ala ggg Gly 180 acc Thr agg Arg cta Le u cga- Ar g gag Glu gt c Val1 gac Asp gac Asp ica Se r ga a Glu 165 cca Pro WO 01/00805 WO 0100805PCT/IBOO/00926 cg c Arg act Th r gg c Gly gtc Val1 230 gaa Giu gta Val1 Gly a cc Th r gca Ala 310 ct C Le u C cg Pro atc Ile gaa Glu ttc Phe tcc Se r 250 cca Pro cgc Arg gtc Val1 gag Glu cca Pro 330 atc Ile gcc Ala cac His caa Gin 235 gga Gly gtg Val1 gac Asp ctc Le u gt c Val1 315 gac Asp gag Glu tcc Se r atc Ile 220 atc Ile aaa Lys ctc Le u atc Ile gag Glu 300 tac Tyr agc Ser tac acc Tyr Thr 190 ggc tat Gly Tyr gcc ctg Ala Leu gac gac Asp Asp ccc ggc Pro Gly 255 gca ctc Ala Leu 270 acc ggc Thr Gly ctc tcc Leu Ser tac atg Tyr Met gtc aag Val Lys 335 aac gta atc cgt gaa aaa Asn Val Ile Arg Glu Lys 195 ttg gga gcc atg cac gca Leu Gly Ala Met His Ala 210 aag aac ttc ggc gca gcc Lys Asn Phe Gly Ala Aia 225 atc atc gac atc ttc tcg Ile Ile Asp Ile Phe Ser 240 245 acc gac ctc cgc gaa ggt Thr Asp Leu Arg Glu Gly 260 cgt gaa gac acc ccc gtc Arg Giu Asp Thr Pro Val 275 cct cta gaa gac gac gag Pro Leu Giu Asp Asp Glu 290 caa tcc ggc gga cgc caa Gin Ser Gly Giy Arg Gin 305 gac atc gcc aac gca gaa Asp Ile Ala Asn Ala Giu 320 325 gaa gcc ctc cgc aac ctt Giu Aia Leu Arg Asn Leu 691 739 787 835 883 931 979 1027 1075 1123 1170 1173 gca acc ttc aca gtc aag cgc gtc gga taaccccgta atccaccact Ala Thr Phe Thr Val Lys Arg Val Gly 345 350 taa <210> 258 <211> 350 <212> PRT <213> Corynebacterium giutamicum <400> 258 Met Ser Ser Gly Arg Thr Val Pro Thr Arg Ser His Gly Leu Gly Lys 1 5 10 Giu Gly Val Ser Thr Thr Gly Ala Ser Gin Val Giu Phe Gly Asp Pro 25 Glu Leu Thr Ala Arg Ile Asn Asp Aia Met Val Gin Val Giu Glu Leu 398 WO 01/00805 PCT/IB00/00926 40 Leu His Thr Glu Leu Ser Ser Gly Glu Asp Phe Leu Val Asp Ile Val 55 Met His Leu Thr Arg Ala Gly Gly Lys Arg Phe Arg Pro Met Phe Ala 70 75 Leu Leu Ala Ser Glu Phe Gly Glu Lys Pro Leu Ser Glu Asn Val Ile 90 Lys Ala Ala Val Val Val Glu Ile Thr His Leu Ala Thr Leu Tyr His 100 105 110 Asp Asp Val Met Asp Glu Ala Ser Met Arg Arg Gly Val Pro Ser Ala 115 120 125 Asn Ala Arg Trp Asp Asn Ser Val Ala Ile Leu Ala Gly Asp Ile Leu 130 135 140 Leu Ala His Ala Ser Gly Leu Met Ser Gin Leu Gly Thr Asp Thr Val 145 150 155 160 Ala His Phe Ala Glu Thr Phe Gly Glu Leu Val Thr Gly Gin Met Arg 165 170 175 Glu Thr Val Gly Pro Arg Asp Thr Asp Pro Ile Glu His Tyr Thr Asn 180 185 190 Val Ile Arg Glu Lys Thr Gly Val Leu Ile Ala Ser Ala Gly Tyr Leu 195 200 '205 Gly Ala Met His Ala Gly Ala Ala Pro Glu His Ile Asp Ala Leu Lys 210 215 220 Asn Phe Gly Ala Ala Val Gly Met Ile Phe Gin Ile Val Asp Asp Ile 225 230 235 240 Ile Asp lie Phe Ser Glu Thr His Glu Ser Gly Lys Thr Pro Gly Thr 245 250 255 Asp Leu Arg Glu Gly Val Phe Thr Leu Pro Val Leu Tyr Ala Leu Arg 260 265 270 Glu Asp Thr Pro Val Gly Ala Glu Leu Arg Asp lie Leu Thr Gly Pro 275 280 285 Leu Glu Asp Asp Glu Thr Val Asn His Val Leu Glu Leu Leu Ser Gin 290 295 300 Ser Gly Gly Arg Gin Ala Ala Leu Asp Glu Val Tyr Arg Tyr Met Asp 305 310 315 320 Ile Ala Asn Ala Glu Leu Asp Arg Leu Pro Asp Ser Thr Val Lys Glu 325 330 335 Ala Leu Arg Asn Leu Ala Thr Phe Thr Val Lys Arg Val Gly 340 345 350 <210> 259 WO 01/00805 <211> 1075 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(1075) <223> FRXA02309 <400> 259 gtgccttccg tcgaetacgg ttaaacaaaa agctttttgt PCT/IB00100926 ccatttcact ggattcaccg aaagaatgaa tccaeactcg ateaceaaag gtagcgatga atg agt agc ggc cga Met Ser Ser Gly Arg cca Pro gca Ala ga c Asp ggg Gly ggc Gly gaa Glu atc Ile t cc Ser 120 gta Val atg Met ggc Giy acc acc Th r t ct Ser gcc Ala gaa Glu aaa Lys aaa Lys acc Thr 105 atg Met gee Al a agt Ser ga a Glu gac cgt Arg ca g Gin at g Met ga e Asp cg a Arg cca Pro ca c His ege Arg atc Ile cag Gin ct a Le u .170 c cg ca c His gag Giu ca g Gin etc Leu cgc Arq tee Ser gcc Ala ggc GI y gca Al a 140 ggt Gi y acc Thr gag ggg Gly ttt Phe gt a Val 45 gte Val ccc Pro ga a Giu ace Thr gte Val1 125 ggc Giy ace Thr gg C Gly cac etc Leu ggt Gly 30 gaa Glu gat Asp atg Met aac Asn ctg Le u 110 eca Pro ga c Asp ga e Asp caa Gin tac gga Gly 15 ga t Asp gaa Giu ate Ile ttt Phe gte Val1 95 tac Tyr agt Se r ate Ile a Ca Thr atg Met 175 ace aaa Lys ccc Pro etc Le u gte Val g ca Ala ate Ile cac His get Al a etc Le u gte Val1 160 ege Arg aac 1 ga a Glu gag Glu etg Le u atg Met etg Le u aaa Lys gac Asp aac Asn ct a Le u 145 gee Al a ga a Glu gta ggt Giy eta Leu cae His eac His ct g Le u gee Ala gat Asp geg Al a 130 gea Ala cac His aca Thr ate gta ValI a eg Thr act Thr eta Leu gee Al a gee Ala gte Val1 115 ega Arg cat His ttt Phe gte Val cgt tee Se r gee Ala ga a Glu aca Thr tee Ser gt t Val1 100 atg Met t gg Trp gca Ala gee Ala ggg Gly 180 ga a ace Thr agg Arg eta Le u ega Arg gag Giu gt C Val1 ga e Asp gac Asp tea Ser ga a Glu 165 eca Pro aaa 115 163 211 259 307 355 403 451 499 547 595 643 691 WO 01/00805 PCT/EBOO/00926 Arg Asp Thr Asp Pro Ile Glu His Tyr Thr Asn Val Ile Arg Glu Lys 185 190 195 act ggt gtc ctc atc qcc tcc gca ggc tat ttg gga gcc atg cac gca 739 Thr Gly Val Leu Ile Ala Ser Ala Gly Tyr Leu Gly Ala Met His Ala 200 205 210 ggc gcc gca cct gaa cac atc gac gcc ctg aag aac ttc ggc gca gcc 787 Giy Ala Ala Pro Glu His Ile Asp Ala Leu Lys Asn Phe Giy Ala Ala 215 220 225 gtc ggc atg atc ttc caa atc gtc gac gac atc atc gac atc ttc tcg 835 Val Gly Met Ile Phe Gin Ile Vai Asp Asp Ile Ile Asp Ile Phe Ser 230 235 240 245 gaa acc cac gaa tcc gga aaa acg ccc ggc acc gac ctc cgc gaa ggt 883 Glu Thr His Giu Ser Gly Lys Thr Pro Gly Thr Asp Leu Arg Glu Gly 250 255 260 gta ttc acc ctc cca gtg ctc tac gca ctc cgt gaa gac acc ccc gtc 931 Val Phe Thr Leu Pro Val Leu Tyr Ala Leu Arg Giu Asp Thr Pro Val 265 270 275 ggc gca gaa ctc cgc gac atc ctc acc ggc cct cta gaa gac gac gag 979 Gly Ala Giu Leu Arg Asp Ile Leu Thr Gly Pro Leu Giu Asp Asp Giu.

280 285 290 acc gtc aac cac gtc ctc gag ctc ctc tcc caa tcc ggc gga cgc caa 1027 Thr Val Asn His Val Leu Giu Leu Leu Ser Gin Ser Gly Gly Arg Gin 295 300 305 gca gcc ctc gac gag gtc tac cgc tac atg gac atc gcc aac gca gaa 1075 Ala Ala Leu Asp Giu Val Tyr Arg Tyr Met Asp Ile Ala Asn Ala Giu 310 315 320 325 <210> 260 <211> 325 <212> PRT <213> Corynebacterium giutamicum <400> 260 Met Ser Ser Gly Arg Thr Val Pro Thr Arg Ser His Gly Leu Gly Lys 1 5 10 Giu Gly Val Ser Thr Thr Gly Ala Ser Gin Val Giu Phe Gly Asp Pro 25 Giu Leu Thr Ala Arg lie Asn Asp Ala Met Val Gin Val Giu Glu Leu 40 Leu His Thr Giu Leu Ser Ser Gly Giu Asp Phe Leu Val Asp Ile Val 55 Met His Leu Thr Arg Ala Gly Gly Lys Arg Phe Arg Pro Met Phe Ala 70 75 Leu Leu Ala Ser Giu Phe Gly Giu Lys Pro Leu Ser Giu Asn Val Ile 90 Lys Ala Ala Val Val Val Giu Ile Thr His Leu Ala Thr Leu Tyr His WO 01/00805 PCT[IBOO/00926 100 105 110 Asp Asp Val Met Asp Glu Ala Ser Met Arg Arg Gly Val Pro Ser Ala 115 120 125 Asn Ala Arg Trp Asp Asn Ser Val Ala Ile Leu Ala Gly Asp Ile Leu 130 135 140 Leu Ala His Ala Ser Gly Leu Met Ser Gin Leu Gly Thr Asp Thr Val 145 150 155 160 Ala His Phe Ala Glu Thr Phe Gly Giu Leu Val Thr Gly Gln Met Arg 165 170 175 Glu Thr Val Gly Pro Arg Asp Thr Asp Pro Ile Giu His Tyr Thr Asn 180 185 190 Val Ile Arg Glu Lys Thr Gly Val Leu Ile Ala Ser Ala Gly Tyr Leu 195 200 205 Gly Ala Met His Ala Gly Ala Ala Pro Giu His Ile Asp Ala Leu Lys 210 215 220 Asn Phe Gly Ala Ala Val Gly Met Ile Phe Gin Ile Val Asp Asp Ile 225 230 235 240 Ile Asp Ile Phe Ser Glu Thr His Giu Ser Gly Lys Thr Pro Gly Thr 245 250 255 Asp Leu Arg Giu Giy Val Phe Thr Leu Pro Val Leu Tyr Ala Leu Arg 260 265 270 Glu Asp Thr Pro Val Gly Ala Giu Leu Arg Asp Ile Leu Thr Gly Pro 275 280 285 Leu Giu Asp Asp Giu Thr Val Asn His Val Leu Giu Leu Leu Ser Gin 290 295 300 Ser Giy Gly Arg Gin Aia Ala Leu Asp Glu Val Tyr Arg Tyr Met Asp 305 310 315 320 Ile Ala Asn Ala Giu 325 <210> 261 <211> 1767 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(1744) <223> RXN00477 <400> 261 tgcgggagcg aatcagagtt ccacttcata tcaaactctc tacactcgct agagccacga tgaaaggtct atctatgagc atctacagaa agaattcgtg atg aag gtc tcg act 115 Met Lys Val Ser Thr 1 WO 01/00805 WO 0100805PCTA~BOO/00926 act Thr ggt Gly gitt Val1 ata Ile ctc Le u act Thr ttt Phe gca Ala gga Gly 135 aga Arg ga t Asp tta Leu ate Ile eca Pro 215 cca Pro ta Le u ttg Leu t ca Se r atg Met t ct Se r tet Se r att Ile 120 aat As n tie Phe gia ValI caa Gin eta Le u 200 t eg Se r ege Arg gee Ala gaa Glu gge Gly ec Pro ga t Asp gg c Gly 105 geg Ala tat Tyr ct t Le u et g Le u aag Lys 185 ace Th r atg Met tee Ser aet Th r aaa Ly s ttt Phe gag Glu tat Tyr aea Th r eta Leu ct t Leu tat Tyr ace Thr 170 tac T yr tat Tyr tae T yr ggt ace gee gta Gly Thr Ala Val 15 gea ett tta gea Ala Leu Leu Ala act gat gte ggt Thr Asp Val Gly 45 gge itt ega tgg Gly Phe Arg Trp 60 ttt gac eat tie Phe Asp His Phe gat ttg gta gaa Asp Leu Val Glu 95 gac get gte gat Asp Ala Val Asp 110 gaa tee ate gaa Giu Ser Ile Giu 125 age geg gca gae Ser Ala Ala Asp 140 aat tte tee aeg Asn Phe Ser Thr get gge ega etg Ala Gly Arg Leu 175 aat agt eaa tte Asn Ser Gin Phe 190 gea gte te etg Ala Val Phe Leu 205 ttg aig agt eat Leu Met Ser His 220 ggt tit act gea Gly Phe Thr Ala gte Val eg t Arg ggC Gly gati Asp tie Phe tta Leu gte Val1 ec Pro gee Ala ta Leu 160 ttt Phe agi Ser tet Ser aee Thr gig Val 240 ata Ile gat Asp ega Arg ace Thr ge a Al a a eg Th r ec Pro gge Giy tat Tyr 145 gge Giy t et Ser age Se r tee Se r ga t Asp 225 gti Val gg e Gly gg e Gly get Al a gga Gi y et t Le u eet Pro act Thr gcg Ala 130 gac Asp ceg Pro eta Leu cg Pro ega Arg 210 ttg Leu aae Asn ggt Gly eaa Gin t eg Se r i ci Ser ggt Gly tat Tyr 100 eg t Arg gea Al a gee Al a ct Leu ac Th r 180 ttg Leu aet Thr eag Gin etg Leu gt t ValI gta Val1 ett Leu i gg Trp g ea Aia ega Arg ga a Giu aaa Lys att Ile eae His 165 eg t Arg ege Arg aet Thr gg a Gly cat His 245 163 211 259 307 355 403 451 499 547 595 643 691 739 787 835 gtg aaa tac cet ata ggt Val Lys Tyr Pro Ile Gly wo 01/00805 WO 0100805PCTJIBOO/00926 cag tta gcg ctg gaa Gin Leu Ala Leu Glu 250 att tcc atc aac act Ilie Ser Ilie Asn Thr 265 gtg agc ttg ctt cac Val Ser Leu Leu His 280 gtg gtt tca gca ggc Vai Vai Ser Ala Gly 295 cgg gaa ctt cga acc Arg Giu Leu Arg Thr 310 gga att gga gcg gta Gly Ile Giy Ala Val 330 cag ctc gac cat cac Gin Leu Asp His His 345 ttt gct gta gtt ttc Phe Ala Vai Val Phe .360 tca aat tcc att tat Ser Asn Ser Ile Tyr 375 gca cct gct gga tac Ala Pro Ala Giy Tyr 390 tct agc agc atc ggc Ser Ser Ser Ile Giy 410 tcc gtg gaa aca atc Ser Vai Glu Thr Ile 425 gcc ggc atc cct gac Ala Giy Ile Pro Asp 440 ggc cct gcg gat ttt Gly Pro Ala Asp Phe 455 cig ggt cca gca cat Leu Gly Pro Ala His 470 aat agc tcc cgc aag aac ggg gtt gag Asn Giy Val Giu ttt caa ctc gat tct gag gtc Phe Gin Leu Asp Ser Giu Vai 255 260 tca Ser aga Arg cta Leu 300 ccc Pro atc Ile ctt Le u ggg Gly t cc Ser 380 aac Asn ggt Gly t ca Ser act Thr cac His 460 ctc Le u aat t cg Ser aaa Lys 285 cac His gaa Giu ctc Leu ttc Phe cct Pro 365 aa g Lys ct t Le u ga t Asp cat His gac Asp 445 cgc Arg aga Arg aac agg Arg 270 g t g Val1 cat His cga Arg ctg Le u ttc Phe 350 caa Gin cct Pro ttt Phe gcg Ala g ca Ala 430 cga Arg tac Tyr ca g Gin ctc ggc Gly caa Gin aca Th r tat T yr ggc Giy 335 agt Se r ctt Leu tca Ser gtt Vali tat Tyr 415 atc Ile att Ile cat His t cc Ser ttc aa c As n aat As n gaa Giu tgg T rp 320 g ta Vali gaa Glu acc Thr a cg Thr tta Leu 400 atg Met aa t As n gtg Val1 t ca S er gct Aia 480 tat aca Thr cta Leu a at Asn 305 tcc Ser aaa Lys ga t Asp cgc Arg t cc Ser 385 att Ile ca g Gin caa Gin gt c Val tgg Trp 465 ttc Phe t cc agc Ser gat Asp 290 aat Asn aat As n g ga Gly t gg Trp ccc Pro 370 gaa Giu ccg Pro t cg Ser att Ile aaa Lys 450 gta ValI tta Le u ggt ggt Gly ctt Leu ccc Pro cc t Pro 325 ccc Pro ga t Asp gca Ala gt t Val1 gcc Al a 405 gca Ala caa Gln att Ile gcg Ala cgc Arg 485 acc 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 WO 01/00805 Asn Ser Ser Arg Lys Vai Asn Asn Leu Phe Tyr 490 495 gtc ccg ggt gta gga ata ccc atg tgt tta att Val Pro Gly Val Gly Ile Pro Met Cys Leu Ile 505 510 att aag cgt tta cat gcc gat acc agt gca gga Ilie Lys Arg Leu His Ala Asp Thr Ser Ala Giy 520 525 ttg ccg cct aaa acg aca cca tct caa aag acc Leu Pro Pro Lys Thr Thr Pro Ser Gin Lys Thr 535 540 taaattttga tccctatcat cga <210> 262 <211> 548 <212> PRT <2i3> Corynebacterium giutamicum Ser Gly Ala Thr 500 tct gcc gag aat Ser Ala Giu Asn 515 cca ctg ccc gaa Pro Leu Pro Giu 530 tca tac gat cat Ser Tyr Asp His 545 PCTLBOO/00926 Thr att 1651 Ile cca 1699 Pro 1744 1767 <400> 262 Met Lys Vai Ser Thr Lys Thr Pro Arg 1 Ile Asp Arg Thr Ala Thr Pro Gly Tyr 145 Gly Ser Ser Gly Giy Aila Gly Leu Pro Th r Ala 130 Asp Pro Le u Pro Ala Trp Gly Pro Phe Gly Gly 115 Giy Ile Leu Leu Val1 Giy Gin Se r Se r Gly Tyr 100 Arg Al a Ala Leu Thr 180 Leu Ala Thr Glu Tyr 70 Cys Vali Glu Leu Asp 150 Arg Ser Gin Gly Val1 Ile 55 Leu Thr Phe Al a Gly 135 Arg Asp Leu Ile Ala 25 Glu Gly Pro Asp Gly 105 Ala Tyr Leu Le u Lys 185 Thr S er 10 Thr Lys Phe Glu Tyr Thr Leu Leu T yr Thr 170 Tyr Tyr Ser Se r Asn Pro Ala 75 Le u His Ph e Asp Asn 155 Arg Val1 Pro Gly Ala Thr Gly Ph e Asp Asp Glu Se r 140 As n Al a Asn Ala Ala Leu Val1 Arg His Val1 Val1 110 Ile Ala Se r Arg Gin 190 Phe Val1 Al a Gly Trp Phe Glu Asp Glu Asp Thr Le u 175 Ph e Leu Val Arg Gly Asp Phe Leu Val Pro Ala Le u 160 Phe Ser S er WO 01/00805 WO 0100805PCTIBOOIOO926 Se r Asp 225 Val1 Le u Th r Le u As n 305 Ser Ly s Asp Arg Ser 385 Ile Gin Gin Val1 Trp 465 Phe Ser Arg 210 Leu As n Asp Ser Asp 290 Asn Asn Gly T rp Pro 370 Glu Pro Ser Ile Lys 450 Val Le u Giy Pro Val1 Al a Ser Ala 275 Ala Le u Arg Glu Th r 355 His Asp Thr Ala Ala 435 Arg Gly Arg Ala Thr Thr Pro Ser Met Tyr His Leu Met Ser His Thr Val 230 Gin Ile Val1 Val Arg 310 Gly Gin Phe Ser Ala 390 Se r Ser Ala Gly Le u 470 Asn Val 215 Lys Leu Ser Ser Val1 295 Glu Ile Le u Al a As n 375 Pro Ser Val1 Gly Pro 455 Gly Ser Pro Pro Ile Leu Glu 250 Asn Thr 265 Leu His Ala Gly Arg Thr Ala Val 330 His His 345 Val Phe Ile Tyr Gly Tyr Ile Gly 410 Thr Ilie 425 Pro Asp Asp Phe Ala His Arg Lys 490 Val Gly 505 Leu His Thr Giu Arg 270 Val1 His Arg Leu Phe 350 Gin Pro Phe Ala Ala 430 Arg Tyr Gin Leu Cys 510 Ser Ala Phe 255 G iy Gin Thr Tyr Gly 335 Ser Le u Se r Val1 Tyr 415 Ile Ile His Ser Phe 495 Leu Ala Vali 240 Gin Asn Asn Giu Trp 320 Val1 Giu Th r Thr Leu 400 Met As n Val1 Se r Ala 480 Tyr Ile Gly Ser Ala Glu Asn Ile Ile Lys 515 WO 01/00805 PCTBOO/00926 Pro Leu Pro Glu Pro Leu Pro Pro Lys Thr Thr Pro Ser Gin Lys Thr 530 535 540 Ser Tyr Asp His 545 <210> 263 <211> 1767 <212> DNA <213> Corynebacterium glutamioum <220> <221> CDS <222> (101)..(1744) <223> FRXA00477 <400> 263 tgcgggagcg aatcagagtt ccacttcata tcaaactctc tacactcgct agagccacga tgaaaggtct atotatgago atctacagaa agaattogtg atg aag gtc tcg act 115 Met Lys Val Ser Thr 1 aaa act cca cgc tcc tca ggt acc gcc gta gtc ata ggc gca ggt gtt 163 Lys Thr Pro Arg Ser Ser Gly Thr Ala Val Val Ile Giy Ala Gly Val 15 got ggt tta goc act tot gca ctt tta gca cgt gat ggc tgg caa gta 211 Ala Gly Leu Ala Thr Ser Ala Leu Leu Ala Arg Asp Gly Trp Gin Val 30 act gtt ttg gaa aaa aat act gat gto gqt ggc cga got gga tog ott 259 Thr Val Leu Glu Lys Asn Thr Asp Val Gly Gly Arg Ala Gly Ser Leu 45 gaa ata toa ggo ttt Oct ggo ttt oga tgg gat aco gga cot tot tgg 307 Glu Ile Ser Gly Phe Pro Giy Phe Arg Trp Asp Thr Gly Pro Ser Trp 60 tac otc atg ccc gag gcc ttt gac cat ttc tto goa Ott ttt ggt gca 355 Tyr Leu Met Pro Giu Ala Phe Asp His Phe Phe Ala Leu Phe Gly Ala 75 80 tgt act tot gat tat otc gat ttg gta gaa tta acg Oct ggt tat oga 403 Cys Thr Ser Asp Tyr Leu Asp Leu Val Giu Leu Thr Pro Gly Tyr Arg 95 100 gtt ttt tot ggc aca oat gao got gto gat gto ccc act ggg cgt gaa 451 Val Phe Ser Gly Thr His Asp Ala Val Asp Val Pro Thr Giy Arg Glu 105 110 115 gaa gca att gog ota tto gaa too ato gaa ccc ggc gog ggt gca aaa 499 Glu Ala Ile Ala Leu Phe Giu Ser Ile Glu Pro Gly Ala Gly Ala Lys 120 125 130 ota gga aat tat ott gat ago gog gca gao gc tat gao att gc att 547 Leu Gly Asn Tyr Leu Asp Ser Ala Ala Asp Ala Tyr Asp Ile Ala Ile 135 140 145 WO 01/00805 PCT/IB0O/00926 gat aga ttc ctt tat aat aat ttc tcc acg tta ggc ccg ctg ctt cac 595 Asp Arg Phe Leu Tyr Asn Asn Phe Ser Thr Leu Gly Pro Leu Leu His 150 155 160 165 cgg gat gta ctg acc cga gct ggc cga ctg ttt tct cta ctg acc cgt 643 Arg Asp Val Leu Thr Arg Ala Gly Arg Leu Phe Ser Leu Leu Thr Arg 170 175 180 tct tta caa aag tac qta aat agt caa ttc agt agc ccg gtg ttg cgc 691 Ser Leu Gin Lys Tyr Val Asn Ser Gin Phe Ser Ser Pro Vai Leu Arg 185 190 195 cag atc cta acc tat cca gca gtc ttc ctg tct tcc cga ccc act act 739 Gin Ile Leu Thr Tyr Pro Ala Val Phe Leu Ser Ser Arg Pro Thr Thr 200 205 210 acc cca tcg atg tac cac ttg atg agt cat acc gat ttg gtg cag gga 787 Thr Pro Ser Met Tyr His Leu Met Ser His Thr Asp Leu Val Gin Gly 215 220 225 gtg aaa tac cct ata ggt ggt ttt act gca gtg qtt aac gct ctg cat 835 Val Lys Tyr Pro Ile Gly Giy Phe Thr Ala Val Vai Asn Ala Leu His 230 235 240 245 caq tta gcg ctg gaa aac ggg gtt gag ttt caa ctc gat tct gag gtc 883 Gin Leu Ala Leu Giu Asn Gly Val Glu Phe Gin Leu Asp Ser Giu Val 250 255 260 att tcc atc aac act gct tca tcg agg ggc aac aca agc gcc aca ggt 931 Ile Ser Ile Asn Thr Ala Ser Ser Arg Gly Asn Thr Ser Ala Thr Gly 265 270 275 gtg agc ttg ctt cac aac aga aaa gtg caa aat cta gat gcg gat ctt 979 Vai Ser Leu Leu His Asn Arg Lys Val Gin Asn Leu Asp Ala Asp Leu 280 285 290 gtg gtt tca gca ggc gac cta cac cat aca gaa aat aat ctg ctt ccc 1027 Val Val Ser Ala Gly Asp Leu His His Thr Giu Asn Asn Leu Leu Pro 295 300 305 cgg gaa ctt cga acc tat ccc gaa cga tat tgg tcc aat cgc aat cct 1075 Arg Giu Leu Arg Thr Tyr Pro Giu Arg Tyr Trp Ser Asn Arg Asn Pro 310 315 320 325 gga att gga gcg gta tta atc ctc ctg ggc gta aaa gga gag tta ccc 1123 Gly Ile Giy Ala Vai Leu Ile Leu Leu Giy Val Lys Gly Giu Leu Pro 330 335 340 cag ctc gac cat cac aac ctt ttc ttc agt gaa gat tgg aca gat gat 1171 Gin Leu Asp His His Asn Leu Phe Phe Ser Giu Asp Trp Thr Asp Asp 345 350 355 ttt gct gta gtt ttc gac ggg cct caa ctt acc cgc ccc cac aat gca i219 Phe Ala Val Val Phe Asp Gly Pro Gin Leu Thr Arg Pro His Asn Ala 360 365 370 tca aat tcc att tat gtc tcc aag cct tca acg tcc gaa gac ggc gtt 1267 Ser Asn Ser Ile Tyr Vai Ser Lys Pro Ser Thr Ser Giu Asp Gly Val 375 380 385 gca cct gct gga tac gaa aac ctt ttt gtt tta att ccg acc aag gcc 1315 WO 01/00805 WO 0100805PCTIBOO/00926 Ala Pro Ala Gly Tyr Glu Asn Leu Phe 390 t ci Se r tcc Ser gcc Ala ggc Gly ci g Le u 470 aat As n gic Val1 at Ile t ig Le u agc Ser gig Val ggc Gly cci Pro 455 gg t Gly a gc Ser ccg Pro aag Lys ccg Pro agc: Ser ga a Giu aic Ile 440 gcg Ala cca Pro.

icc Ser ggt Gly cgi Arg 520 cci Pro aic Ile a ca Thr 425 cci Pro gai Asp g ca Ala cg c Ar g gia Val 505 ta Le u aaa ggc Gly.

410 aic Ile gac Asp iii Phe cat His aag Lys 490 gga Gly cat His a cg 395 cac His gcg Ala ctc Leu gag Glu acc Th r 475 gic Val1 aia Ile gcc Aia aca gg t Gly ica Ser act Thr cac His 460 ctc Leu aat Asn ccc Pro gat Asp cca ga t Asp cat His gac Asp 445 cgc Arg a ga Arg aac Asn aig Mei acc Thr 525 ict Ser Le u 400 aig Met aat Asn gig Val1 t ca Ser gct Ala 480 tat Tyr ati Ile gga Gi y acc Thr Ile ca g Gin caa Gin gic Val1 t g Trp 465 ttc Phe icc Ser ict Ser cca Pro i ca Ser 545 Pro i cg Ser ati Ile aaa Lys 450 gia Val1 tta Leu ggi Gly gcc Ala cig Leu 530 i ac T yr Thr gci Ala gct Ala 435 cgc Arg gg c Gly aga Arg gcc Ala gag Giu 515 ccc Pro gati Asp Lys t ca Ser 420 a cg Thr acc Thr agi Ser ggg Gly acc Thr 500 aat As n ga a Giu cat His Ala 405 gca Ala ca a Gin at Ile gcg Ala cg c Arg 485 acc Th r ati Ile cca Pro 1363 1411 1459 1507 1555 1603 1651 1699 1744 1767 Lys Thr Thr Pro 540 535 taaattttga tccctatcai cga <210> 264 <211> 548 <212> PRT <213> Corynebacterium giutamicum <400> 264 Met Lys Val Ser Thr Lys Thr Pro Arg 1 5 Ile Gly Ala Gly Val Ala Gly Leu Ala 25 Asp Gly Trp Gin Val Thr Val Leu .Giu 40 Arg Ala Gly Ser Leu Giu Ile Ser Gly 55 Thr Gly Pro Ser Trp Tyr Leu Met Pro Se r 10 Thr Lys Phe Giu Ser Gly Thr Ala Val Val Ser Ala Leu Leu Ala Arg Asn Thr Asp Val Giy Gly Pro Gly Phe Arg Trp Asp Ala Phe Asp His Phe Phe WO 01/00805 WO 0100805PCT[IBOO/00926 Al a Th r Pro Gly Ty r 145 Gly Ser Se r Ser Asp 225 Val Leu Thr Leu As n 305 Ser Lys Asp Arg Se r 385 Leu Pro Thr Ala 130 Asp Pro Leu Pro Arg 210 Leu Asn Asp S er Asp 290 Asn As n Gly Trp Pro 370 Glu Ph e Gly Gl y 115 Gly Ile Leu Leu Val1 195 Pro Val1 Ala Se r Ala 275 Al a Leu Arg Giu Thr 355 His Asp Gly Tyr 100 Arg Al a Al a Le u Th r 180 Le u Th r Gin Le u Giu 260 Thr Asp Le u Asn Le u 340 Asp As n Gly Ala Arg Glu Lys Ile His 165 Arg Arg Th r Gly His 245 Val1 Gly Le u Pro Pro 325 Asp Ala Val Cys Val1 Glu Le u Asp 150 Arg Ser Gln Th r Val1 230 Gin Ile Val1 Val1 Arg 310 G ly Gln Phe Se r Ala 390 Thr Phe Ala Gly 135 Arg Asp Leu Ile Pro 215 Lys Leu Ser S er Val1 295 Glu Ile Leu Ala Asn 375 Pro Ser Se r Ile 120 Asn Phe Val1 Gln Le u 200 Se r Tyr Ala Ile Leu 280 Ser Leu Gly Asp Val1 360 Ser Ala Asp Gly 105 Al a Tyr Leu Leu Lys 185 Thr Met Pro Le u As n 265 Leu Al a Arg Al a His 345 Val Ile Gly T yr 90 Thr Le u Le u Tyr Th r 170 Tyr Tyr Tyr Ile Giu 250 Thr His Gly Thr Val1 330 His Phe Tyr Tyr Le u His Phe Asp Asn 155 Arg Val1 Pro His Gly 235 As n Ala Asn Asp Tyr 315 Leu As n Asp Val1 Giu 395 Asp Asp Giu Ser 140 As n Al a Asn Ala Leu 220 Gly Gly Ser Arg Leu 300 Pro Ile Leu Gly Ser 380 Asn Leu Val Ala Val 110 Ser Ile 125 Ala Ala Phe Ser Gly A rg Ser Gln 190 Val Phe 205 Met Ser Phe Thr Val Giu Ser Arg 270 Lys Vai 285 His His Glu Arg Leu Leu Phe Phe 350 Pro Gin 365 Lys Pro Leu Phe Glu Leu Asp Val Giu Pro Asp Ala Thr Leu 160 Leu Phe 175 Phe Ser Leu Ser His Thr Ala Val 240 Phe Gin 255 Gly Asn Gin Asn Thr Giu Tyr Trp 320 Gly Val 335 Ser Giu Leu Thr Ser Thr Val Leu 400 WO 01/00805 Ile Pro Thr Lys Ala Ser Ser Ser Ile Gly His 405 410 Gin Ser Ala Ser Ala Ser Val Giu Thr Ile Ala 420 425 Gin Ilie Ala Thr Gin Ala Gly Ile Pro Asp Leu 435 *440 Val Lys Arg Thr Ile Gly Pro Ala Asp Phe Glu 450 455 Trp Val Gly Ser Ala Leu Gly Pro Ala His Thr 465 470 475 Phe Leu Arg Gly Arg Asn Ser Ser Arg Lys Val 485 490 Ser Gly Ala Thr Thr Val Pro Gly Val Gly Ile 500 505 Ser Ala Glu Asn Ile Ile Lys Arg Leu His Ala 515 520 Pro Leu Pro Glu Pro Leu Pro Pro Lys Thr Thr 530 535 Ser Tyr Asp His 545 <210> 265 <211> 954 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (931) <223> RXA00478 <400> 265 accagaagca gcctcagcta tgacacacca. aaattcgcct gagactttac aatcgggcct cattcaaggc ttcacataaa T yr 415 Ile Ile His Ser Ph e 495 Leu Ala Lys PCTIBOO/00926 Met As n Val1 Se r Ala 480 Tyr Ile Gly Thr tcc acq agc ttc agt ctg tct Ser Thr Ser Phe Ser Leu Ser aat gac ata cga aat ctc tat Asn Asp Ile Arg Asn Leu Tyr gtc gac ggc act gca cat gcc Val Asp Gly Thr Ala His Ala tgg ttg cta Trp Leu Leu 15 gta gtt cgt Val Val Arg 30 ggt tgc tca Gly Cys Ser ctcttcctta aaagtgcact qtg atc gaa gaa tat Val Ile Giu Giu Tyr 1 tcc cca cgc ata cga Ser Pro Arg Ile Arg atc gcc gat gag att Ile Ala Asp Giu Ile act gcc aaa atc gaa Thr Ala Lys Ile Glu 115 163 21.1 259 WO 01/00805 gag att ctc g( Glu Ile Leu A~ cgc ttc aac a~ Arg Phe Asn T~ cgc tgt gat ti Arg Cys Asp P~ cgt aag gac ct Arg Lys Asp LE 1 tat gtc tat g Tyr Val Tyr G1 120 ttc aac caa g Phe Asn Gin G] 135 aac gga gcc cc Asn Gly Aia Ar 150 cgt gac ttg gc Arg Asp Leu Al aaa acc agc ca Lys Thr Ser Gl 18 gct gat atc cc Ala Asp Ile Ar 200 ata cca gtg ca Ile Pro Vai Gl 215 caa aaa ctc ac Gin Lys Leu Th 230 cgg gag cga at Arg Giu Arg Il aga gcc acg at Arg Ala Thr Me at gcc tat sp Ala Tyr ca gat ctt hr Asp Leu 75 tc gaa caa ie Glu Gin *c aaa gct *u Lys Ala '5 ;c tcc gcg -y Ser Ala ;t aga acg -y Arg Thr jc tca ttg ~g Ser Leu 155 "a gaa gat a Giu Asp 170 "a gga act "n Giy Thr ;t caa gac ~g Gin Asp ig gct cgc .n Ala Arg ~t gac cga *r Asp Arg 235 *c aga gtt *e Arg Val 250 :g aaa ggt ~t Lys Gly ga a Glu 60 gt t Val1 gag Giu aat Asn ga a Giu att Ile 140 gga Giy cag Gin ct t Leu cta Leu atc Ile 220 att Ile cca Pro cta att Ile tta Leu cat His a ca Thr gtt Val 125 a gc Ser gcg Al a caa Gin act Th r gca Ala 205 gg a Gly gag Glu ctt Leu g cg Ala caa Gin gta Val1 cac His 110 ata Ile aaa Lys gca Ala aat As n aaa Lys 190 att Ile g tg Val gct Ala cat His gtt Val1 gc t Ala ata Ile 95 gac Asp ggC Gi y aaa Lys ttc Phe t tg Leu 175 gaa Glu gcc Ala a tc Ile act Thr atc Ile 255 ct t Le u tat Tyr 80 gcc Al a cca Pro ctg L eu cgg Arg ca g Gin 160 ggc Gly caa Gin cac His tct Se r cct Pro 240 aaa Lys gca Ala ggt Gly ttc Ph e ga t Asp ct t Le u cta Leu 145 aaa Lys cga Arg aaa Lys ga t Asp gct Ala 225 acc Thr ct c Le u gca cca Ala Pro gaa act Glu. Thr ttt gca Phe Ala agc ttc Ser Phe 115 tgt ctc Cys Leu 130 gag att Glu Ile att aac Ile Asn ttt tat Phe Tyr gaa gat Glu Asp 195 gca ttt Ala Phe 210 tat ttg Tyr Leu gcc gat Ala Asp tct aca Ser Thr caa Gin gcc Ala t ca Ser 100 a ca Thr agc Ser a tg Met ttt Phe ttc Phe 180 ct c Le u cca Pro ctc Leu tta Leu ct c Leu 260 PCT/IBOO/00926 caa 307 Gin cga 355 Arg atg 403 Met acg 451 Th r gtt 499 Val caa 547 Gin ctc 595 Le u 165 ccc 643 Pro atc 691 Ile gaa 739 Giu ttt 787 Phe ttg 835 Le u 245 gct 883 Ala tct atg agc atc tac aga aag aat tcg Leu Ser Met Ser Ile Tyr Arg 270 Lys Asn Ser 275 265 tgatgaaggt ctcgactaaa act <210> 266 <211> 277 WO 01/00805 <212> PRT <213> Corynebacterium giutarnicum <400> 266 Val Ile Giu Giu Tyr Ser Thr Ser Phe PCT/BOOIOO926 1 Ser Pro Arg Ilie Ala Asp Thr Ala Lys Ala Ala Pro Gly Glu Thr Phe Phe Ala Asp Ser Phe 115 Leu Cys Leu 130 Leu Giu Ile 145 Lys Ile Asn Arg Phe Tyr Lys Giu Asp 195 Asp Ala Phe 210 Ala Tyr Leu 225 Thr Ala Asp Leu Ser Thr Tyr Arg Lys 275 <210> 267 <211> 1056 5 Ile Arg Giu Ile Ile Giu Gin Gin Ala Arg Ser Met 100 Thr Thr Ser Val Met Gin Phe Leu 165 Phe Pro 180 Leu Ile Pro Giu Leu Phe Leu Leu 245 Leu Aia 260 Asn Ser Asn Val Giu Arg 70 Arg Ar cg Tyr Ph e Asn 150 A rgc Ly s Al a Ilie Gin 230 Arg Asp Asp Ile 55 Phe Cys Lys Val As n 135 Gly Asp Th r Asp Pro 215 Lys Glu Ile Gly 40 Leu Asn Asp Asp Tyr 120 Gin Ala Leu Ser Ile 200 Val1 Leu Arg Arg Th r Asp Thr Phe Leu 105 Giy Gly Arg Ala Gin 185 Arg Gin Thr Ile Ser As n Al a Ala Asp Giu Lys Ser Arg Ser Giu 170 Gly Gin Aila Asp Arg 250 Le u Leu His T yr Leu Gin Aila Ala Thr Leu 155 Asp Thr Asp Arg Arg 235 Val1 Ser T yr Ala Giu Val Giu Asn Giu Ile 140 Gly Gin Leu Le u Ile 220 Ile Pro Th r Aia Ala Ile Leu His Thr Val1 125 Ser Aia Gin Th r Ala 205 Giy Giu Leu Arg Ala Thr Met Lys Giy Leu Ser Met Ser Ile WO 01/00805 <212> DNA <213> Corynebacterium giutarnicum <220> <221> CDS <222> (101)..(1033) <223> RXA01291 <400> 267 aacccgcagc gggtggagcg gggctgaggg agacgtcgag PCT/IBOO/00926 aagcgtccct ttcggttgtc tggatttctc gcggtaaagi ttcctgtgaa gga ga t Asp gta Val1 t cg Ser gtg Val1 C C9 Pro ctc Le u ggc Gly ttt Ph e act Th r atg Met 150 cgg Arg a ag Lys ttt Phe atc Ile ccg Pro gCC Ala ga c Asp agt Ser aat As n tt Phe ca 9 Gin 135 igt Cys g CC Ala gta Val ttg Leu gcc Ala aaa Lys ga t Asp 9CC Al a tig Leu acc Th r ga t Asp 120 ttg Le u tig Leu acg Thr aat Asn ttt Phe icc Ser cg C Arg gt g Val1 gag Giu 75 CC t Pro aaa Lys cg C Arg tac Tyr ttc Phe 155 cac His cg a Arg.

qati Asp t CC Ser gat Asp gac Asp 60 att Ile ttt Phe tgt Cys cgc Arg att Ile 140 ctc Leu gg C Gly ga t Asp gcg Ala agt Se r att Ile 45 ggc Gi y ttq Leu cat His 99 t Gly gati Asp 125 t ac Tyr caa Gin gcc Ala cta Leu a tg Met ttt Phe gag Giu gct Ala ga c Asp acc Th r tt Phe 110 ctc Le u Gi y gat Asp agg Ar g gc t Ala ~ggcgagt gtg gct gat cag caa Val Ala Asp Gin Gin 1 agc tca aag gcg acg gcc acg Ser Ser Lys Ala Thr Ala Thr act Ctg gca tcg aag ctg tig Thr Leu Ala Ser Lys Leu Leu gca ttg tac gca aig gig cga Ala Leu Tyr Ala Met Val Arg gct gct gcc gcg ggg igt gcg Ala Ala Ala Ala Gly Cys Ala aat tac gaa cgc cag git ctg Asn Tyr Giu Arg Gin Val Leu 80 gai cca gtg atc cat gcg ttt Asp Pro Val Ile His Ala Phe 100 gag cag gct cac atc gig gcg Glu Gin Ala His Ile Val Ala 115 tcc caa acc icc tat gat ccg Ser Gin Thr Ser Tyr Asp Pro 130 icc gct gag gtc atc ggg tig Ser Ala Glu Val Ile Gly Leu 145 icc act gcc agc ccg cag gat Ser Thr Ala Ser Pro Gin Asp 160 165 cgi tig ggi gcg gca iii caa Arg Leu Gly Ala Ala Phe Gin 175 180 gaa gac cgc gaa ggc cia ggc Giu Asp Arg Giu Gly Leu Gly 115 163 211 259 307 355 403 451 499 547 595 643 691 WO 01/00805 PCT/IB00/00926 185 190 195 cgt tct tat ttg ccg gta ttc acc gaa gaa atg cgc gat gag atc gtt 739 Arg Ser Tyr Leu Pro Val Phe Thr Glu Glu Met Arg Asp Glu Ile Val 200 205 210 act gat atc cga gag gat ttg gat gcc gcc cgg ctg age att ccg ctg 787 Thr Asp Ile Arg Glu Asp Leu Asp Ala Ala Arg Leu Ser Ile Pro Leu 215 220 225 ctg cca ttt ggc gcg cga acc ggt gtg cgc gcc gcg acc gat ctc tac 835 Leu Pro Phe Gly Ala Arg Thr Gly Val Arg Ala Ala Thr Asp Leu Tyr 230 235 240 245 ggt tgc ctc gtg gac aac ctg gaa tec gcg tcc ctc gaa gac tta aaa 883 Gly Cys Leu Val Asp Asn Leu Glu Ser Ala Ser Leu Glu Asp Leu Lys 250 255 260 aac ggg cgg gat ttt gtg ccg tct tta aaa aag cca gcc tgg caa cca 931 Asn Gly Arg Asp Phe Val Pro Ser Leu Lys Lys Pro Ala Trp Gin Pro 265 270 275 aag caa tgt gga aag aag tgt ttc aaa aat gac aaa agc agt ggt cat 979 Lys Gin Cys Gly Lys Lys Cys Phe Lys Asn Asp Lys Ser Ser Gly His 280 285 290 cgg cgg ggg act agc agg act agc cac cac cgc act get ctt acg cga 1027 Arg Arg Gly Thr Ser Arg Thr Ser His His Arg Thr Ala Leu Thr Arg 295 300 305 agg ata tgaagtccac ctcgtcgaac aaa 1056 Arg Ile 310 <210> 268 <211> 311 <212> PRT <213> Corynebacterium glutamicum <400> 268 Val Ala Asp Gin Gin Asp Phe Leu Gly Arg Phe Asp Ala Met Ser Ser 1 5 10 Lys Ala Thr Ala Thr Val Ile Ala His Tyr Ser Ser Ser Phe Thr Leu 25 Ala Ser Lys Leu Leu Ser Pro Lys Ile Arg Arg Asp Ile Glu Ala Leu 40 Tyr Ala Met Val Arg Val Ala Asp Glu Val Val Asp Gly Ala Ala Ala 55 Ala Ala Gly Cys Ala Pro Asp Ala Val Ala Glu Ile Leu Asp Asn Tyr 70 75 Glu Arg Gin Val Leu Leu Ser Leu Ser Val Pro Phe His Thr Asp Pro 90 Val Ile His Ala Phe Gly Asn Thr Ala Arg Lys Cys Gly Phe Glu Gin 100 105 110 WO 01/00805 WO 0100805PCTA~BOOIOO926 Ala His Ile Val Ala Phe Phe Asp Ser Met Arg Arg Asp Leu Ser Gin 115 120 125 Thr Ser Tyr Asp Pro Thr Gin Leu Asp Giu Tyr Ile Tyr Gly Ser Ala 130 135 '140 Glu Val Ile Gly Leu Met Cys Leu Lys Ile Phe Leu Gin Asp Ser Thr 145 150 155 160 Ala Ser Pro Gin Asp Arg Ala Thr Met Giu His Giy Ala Arg Arg Leu 165 170 175 Gly Ala Ala Phe Gin Lys Val Asn Phe Leu Arg Asp Leu Ala Glu Asp 180 185 190 Arg Giu Gly Leu Gly Arg Ser Tyr Leu Pro Val Phe Thr Glu Glu Met 195 200 205 Arg Asp Giu Ile Val Thr Asp Ile Arg Glu Asp Leu Asp Ala Ala Arg 210 215 220 Leu Ser Ile Pro Leu Leu Pro Phe Gly Ala Arg Thr Gly Val Arg Ala 225 230 235 240 Ala Thr Asp Leu Tyr Giy Cys Leu Val Asp Asn Leu Glu Ser Ala Ser 245 250 .255 Leu Giu Asp Leu Lys Asn Gly Arg Asp Phe Val Pro Ser Leu Lys Lys 260 265 270 Pro Ala Trp Gin Pro Lys Gin Cys Gly Lys Lys Cys Phe Lys Asn Asp 275 280 285 Lys Ser Ser Gly His Arg Arg Gly Thr Ser Arg Thr Ser His His Arg 290 295 300 Thr Ala Leu Thr Arg Arg Ile 305 310 <210> 269 <211> 1239 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1216) <223> RXA00480 <400> 269 tttatggacc caaattcaca ctttctgtac ttcatcaaaa taaagcgcca tatacaacga ttgggaattt ttgcgagata actggccgtg tgatactcga atg gac aat ggc atg 115 Met Asp Asn Gly Met 1 aca atc acc aca gaa cat tca act cat cct gat ctt gat ttc aat gat 163 Thr Ile Thr Thr Giu His Ser Thr His Pro Asp Leu Asp Phe Asn Asp 15 WO 01/00805 WO 0100805PCT[IBOO/00926 gag Glu tgc Cys caa Gin cta Le u gtt Val1 gt t Val aat Asn caa Gin ct g Le u 150 cgc Arg aac Asn tta Leu agt Ser gcg Ala 230 gag Glu att Ile agc Ser gct Ala tac Tyr tta Leu cat His ttt Phe atc Ile 135 ctt Leu ctt Leu cac His agc Se r c9g Arg 215 gca Ala ata Ile tat Tyr aca Thr ttg Leu aac Asn ca g Gin gac Asp att Ile 120 c ag Gin a tg Met gac Asp a cg Thr agc Ser 200 cta Leu att Ile Gly cgg Arg tat Tyr cga Arg acg Thr gt a Val1 ga t Asp 105 ggg Giy cgc Arg gga Gly ctt Leu ata Ile 185 aaa Lys aaa Lys ctc Leu aca Thr gaa Glu caa Gin ggt Gly ctt Le u gca Ala gtt Val ca g Gin gat Asp aat As n cca Pro 170 aa t As n gc C Ala aca Thr gcg Ala aa C Asn 250 cta Leu C ca Pro ggc Gly gta Val gtg Val1 att Ile att Ile cca Pro ctt Le u 155 cat His gac Asp atc Ile gcc Ala ga a Giu 235 ttg Leu aac As n gag Glu aag Lys gac Asp gCt Al a ga C Asp Ctc Le u gag Glu 140 ttt Ph e cac His act Th r agc Ser aca Thr 220 cta Le u ggc Gly cta Leu gat Asp atg Met aaa Lys ttt Phe c ga Arg ga a Giu 130 caa Gin caa Gin tta Le u ctt Le u gct Ala 210 cca Pro aca Thr cag Gin tct Se r gct Ala cta Le u ctc Leu t cc Ser ggc Gly 115 ag t Ser t ct Ser atc Ile ga t Asp ga t Asp 195 cta Le u atg Met aag Lys ga c Asp WO 01/00805 PCT/IB00/00926 tta tct act ttt ggt gac gca gcc gaa cac ggc aaa gat gcc ttt tct 931 Leu Ser Thr Phe Gly Asp Ala Ala Glu His Gly Lys Asp Ala Phe Ser 265 270 275 gac ctt cga gaa gga aaa gaa act aca att atc gcc ttc gct cga gat 979 Asp Leu Arg Glu Gly Lys Glu Thr Thr Ile Ile Ala Phe Ala Arg Asp 280 285 290 act gct aaa tgg act gat att caa gac aac ttc ggc tcc gca gat ctg 1027 Thr Ala Lys Trp Thr Asp Ile Gin Asp Asn Phe Gly Ser Ala Asp Leu 295 300 305 age acc tct cag gca gag cga att caa cat ctt ctc ata cag tgt gga 1075 Ser Thr Ser Gin Ala Glu Arg Ile Gin His Leu Leu Ile Gin Cys Gly 310 315 320 325 gca aag aat cac tcc ttg aat gcc ate tcc gac cac tta aat atc tgc 1123 Ala Lys Asn His Ser Leu Asn Ala Ile Ser Asp His Leu Asn Ile Cys 330 335 340 cgt tcg atg atc aaa aca cta agc ccc cag gta gat ccc aag get caa 1171 Arg Ser Met Ile Lys Thr Leu Ser Pro Gin Val Asp Pro Lys Ala Gin 345 350 355 aat tta tta ctt aaa caa gtt gag caa cta gcc agc cgc aaa tct 1216 Asn Leu Leu Leu Lys Gin Val Glu Gin Leu Ala Ser Arg Lys Ser 360 365 370 tagaactaac ctttacgcct tta 1239 <210> 270 <211> 372 <212> PRT <213> Corynebacterium glutamicum <400> 270 Met Asp Asn Gly Met Thr Ile Thr Thr Glu His Ser Thr His Pro Asp 1 5 10 Leu Asp Phe Asn Asp Glu Ile Tyr Arg Glu Leu Asn Arg Ile Cys Ala 25 Ser Leu Ser Gin Gin Cys Ser Thr Tyr Gin Pro Glu Phe Arg Thr Cys 40 Leu Asp Ala Ala Phe Gin Ala Leu Arg Gly Gly Lys Leu Ile Arg Pro 55 Arg Met Leu Leu Gly Leu Tyr Asn Thr Leu Val Asp Asp Asp Ile Glu 70 75 Val Lys Leu Asn Thr Val Leu Gin Val Ala Val Ala Leu Glu Leu Leu 90 His Phe Ser Leu Leu Val His Asp Asp Val Ile Asp Gly Asp Leu Tyr 100 105 110 Arg Arg Gly Lys Leu Asn Phe Ile Gly Gin Ile Leu Met His Arg Thr 115 120 125 WO 01/00805 WO 0100805PCT/IBOO/00926 Pro Glu Ser Phe Ala Gin Ile Gin Arq Asp Pro Glu His Leu Asp Trp 130 Gin Gin Le u Le u Ala 210 Pro Thr Gin Asp Phe 290 Ser Ilie Leu Pro Arg 370 Ser Ile Asp Asp 195 Le u Met Lys Asp Ala 275 Al a Ala Gin Asn Lys 355 Lys Asn Phe L eu 180 Val Glu Arg Ile Asp 260 Phe Arg Asp Cys Ile 340 Ala Ser Gly Leu Ile 185 Lys Lys Leu Thr Ph e 265 6lu Trp Gin His Ile 345 Leu Asn Pro 170 Asn Ala Th r Ala Asn 250 Gi y Gi y Thr Ala Ser 330 Lys L ys Leu 155 His Asp Ile Ala Giu 235 Le u Asp Lys Asp Giu 315 Le u Th r Gin 140 Phe His Thr Ser Thr 220 Leu Gly Al a Glu Ile 300 Arg As n Leu Val1 Leu Gin Ile Pro 205 Tyr Pro Ile Ala Thr 285 Gin Ile Ala Ser Giu 365 <210> 271 <211> 1056 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (1033) <223> RXS01879 <400> 271 ctttgcgggc cgctgatatt gatccaacgc ttcgtggcga aaagcttgat gtcactgact WO 01/00805 atgtgcgcct agctggggtg ttgcagcaaa aggatgagaa PCTIBOO/00926 gct 115 Al a aa g Lys gac Asp ttt Phe ggg Gly gag Glu cgg Arg aag Lys gct Ala ga c Asp tgc Cys 150 gta Val1 g cg Ala aat As n acc Thr gcg Ala gat Asp gac Asp agc Se r gac Asp cgc Arg ggg Gly gcg Ala aca Thr 135 ctg Leu ga t Asp cat His ccg Pro ggc Gly 215 t gg Trp gga Gly acc Thr gtC Val1 gcc Ala g cg Ala att Ile aca Thr 120 ttg Leu ct t Leu atg Met ggc Gly gaa Giu 200 aac Asn g cg Ala ttt Ph e gtc Val1 gtc Val1 agc Ser gaa Giu ccg Pro 105 ct g Leu ct g Le u gg t Gly t tg Leu ctg Leu 185 t cg Ser a cc Thr aaa Lys ca c His acc Thr aa g Lys aat As n aag Lys gtg Val1 cg c Arg gag Giu Giy acg Thr 170 tcc Ser cat His gcc Ala acc Th r gag Giu tta Le u caa Gin ct t Le u 75 a cg Thr gct Ala gca Ala gtt Val1 acc Thr 155 cgc Arg a cg Thr atg Met gag Glu aac Asn ctc Le u acc Thr tta Leu 60 gcg Ala ccg Pro gg c Gly gtg ValI gcc Ala 140 atg Met ggc Gly cct Pro ga t Asp gtg Val1 220 ctg Leu atg Met acc Thr tct.

Ser tgg Trp ctg Le u gg C Gly gat Asp 125 gcg Ala cg c Ar g aag Lys gag Glu atc Ile 205 ggg Gly cat His a cg Th r ctc Le u gtg Val cg c Arg tct Ser atg Met 110 gcc Ala gag Glu ggt Gly cta Leu gta Val1 190 agc Ser cag Gin tta Leu gtg Val ga t Asp acc Thr gct Ala g ca Ala gct Ala t gg Trp ctc Leu acc Thr cat His 175 ttc Ph e ga c Asp tgg Trp ggt Gly ttt Phe gag Glu gg t Giy gtg Val1 80 gt t Val1 ggc Gly att Ile gqC Gly gg t Gly 160 tgg Trp aaa Lys ctc Leu ctg Leu gtg Val1 1 g tg Val1 caa Gin gag Glu gcc Ala ga t Asp t cg Ser ggc Gi y ggg Gi y t ca Ser 145 cgc Arg gtg Val1 aa g Lys ag c Ser cac His 225 cac His ctg Leu gag Giu cct Pro aa g Lys tcC Ser gcg Ala gag Glu ttt Phe ctg Le u 165 atg Met ctg Le u ctc Leu acc Thr aaa att acc Lys Ile Thr 163 211 259 307 355 403 451 499 547 595 643 691 739 787 WO 01/00805 agc gcc gca ctc agt ttg cgc cct gaa Ser Ala Ala Leu Ser Leu Arg Pro Glu 230 235 ggc atc cgc tcc ggc gcg cat gca gga Gly Ile Arg Ser Giy Ala His Ala Gly 250 acc acg gta ttc ttg tgc gaa tcg gag Thr Thr Val Phe Leu Cys Giu Ser Glu 265 270 gag gcg -cta atc gac gcc ggc cag gtg Glu Ala Leu Ile Asp Ala Gly Gin Val 280 285 ggc cct gcg gcc tca acc gcc gac cag Gly Pro Ala Ala Ser Thr Ala Asp Gin 295 300 gtt tca taataaagac aaacttaagt atc Val Ser 310 <210> 272 <2i1> 311 <2i2> PRT <213> Corynebacterium giutamicum <400> 272 Val Lys Ilie Thr Ala Lys Ala Trp Ala ct g Leu att Ile 255 cac His tac Tyr cg c Arg cgc Arg 240 gtc Val1 aaa Lys gct Ala 99C Giy ctc Leu tc Ser ga c Asp 275 acc Thr att Ile Ca a Gin Gly 260 gtt Val1 gc Ala t tg Leu PCTIBOOIOO926 gaa 835 Giu 245 ccg 883 Pro aaa 931 Lys acc 979 Th r act 1027 Th r 1056 Val Gin G iu Ala Asp Ser Gly Gly Ser 145 Gly Th r Leu Arg Al a Leu Se r Pro 130 Asp Asp Asp Ser Asp Arg Gly Ala Thr 135 Leu Giy Th r Vai Ala Ala Ile Th r 120 Le u Le u Lys His Thr Lys Asn Ly s ValI Arg Glu Gly Thr Giu Leu Gin Leu 75 Thr Al a Ala Val1 Thr 155 Leu Met Thr Ser Trp Leu Gly Asp 125 Ala Arg His Th r Leu Val Arg Se r Met 110 Ala Giu Gly Leu Val1 Asp Thr Ala Ala Ala T rp Leu Thr Gly Ph e Giu Gly Val Val Gly Ile Gly Gly 160 wo 01/00805 PCT[IBOO/00926 Arg Gly Glu Gin Leu Val Asp Met Leu Thr Arg Gly Lys Leu His Trp Val Lys Se r His 225 Ser Se r Al a Al a Al a 305 Val1 His Ala 210 Asn Val1 Gly Gin Tyr 290 His 165 Met Ala Leu Asn Leu Thr Thr Ser 230 Glu Gly 245 Pro Thr Lys Glu Thr Gly Thr Val 310 170 175 Phe Asp T rp Leu Ile 255 His Tyr Arg Lys Leu Leu Arg 240 Val1 Lys Ala Gly <210> 273 <21 1> 891 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (868) <223> RXS02023 <400> 273 gatgtggcag caattttgaa gcagtacctg agcgagtaac gacttccgaa atgtaactag agactagagg aqgaaaoaog ccc Pro cgg Arg ga t Asp tot ago Ser cag Gin cac His gag aat Asn act Th r cac His gg t ogcattcggg gttatcgtgg atg got cct aaa caa Met Ala Pro Lys Gin 1 gga oca gtt ctg caa Gly Pro Val Leu Gin ttg ota gaa atg ogc Leu Leu Glu Met Arg.

cgt gta ctg ogt att Arg Val Leu Arg Ile cao gag atg cca aag 115 163 211 259 307 WO 01/00805 WO 0100805PCTIIBOOIOO926 Gin Ser Glu Phe Val Ala Gl y Phe Asp Ala Leu His Glu Met Pro Lys gcc Al a tac Tyr gca Al a gg g Gly cca Pro cgg Arg 150 ttt Phe gt c Val ct g Leo cgc Arg t tg Leu 230 acc Thr g cg Ala gtc Val1 agc Se r 120 gaa Glu ttc Phe tgt Cys tgc Cys ggc Gly 200 gta Val act Thr gtc Val ggt Gly acc Th r 105 gag Glu ca g Gin ttc Phe ctg Leu at g Met 185 act Thr gag Glu gat Asp ttt Ph e gta Val1 gg t Gly g cc Ala cat His gca Al a cct Pro 170 gta Val1 gag Glu gaa Glu gac Asp gg t Gly ga a Glu Gly aat Asn ctg Leo cg c Arg ggt Gly caa Gln ttc Ph e gqc Gly ct g Leu 235 gca Ala gg t Gly cca* Pro tta Leu 125 cc t Pro acc Thr t tc Phe ggc Gly g ca Ala 205 atc Ile cag Gln gag Glu gtt Val gaa Glu ctg Leo ttg Leo 145 aaa Lys ga t Asp aac As n ga t Asp ga t Asp 225 ttc gat Asp gca Ala gcc Ala ggc Gly 130 ggt Gly tac Tyr gag Glu ttt Phe t gg T rp 210 gtt Val1 atc Phe Ile Val Asp Ala 245 cac gct gga ttg gac gta gcg cgt ctc cac aat taagcagtgg ctacattagg 888 His Ala Gly Leo Val Ala Arg Leo His Asn t gt <210> 274 <211> 256 <212> PRT <213> Corynebacteriom glutamicum <400> 274 Met Ala Pro Lys Gln Thr Pro Ser Pro Glu Lys Asn Arg Asn Leo Val WO 01/00805 WO 0100805PCTAIBOOIOO926 Gly Le u Arg His Glu Val1 Giu Leu Leu 145 Ly s Asp As n Asp Asp 225 Pro Le u Val1 Glu Asp Al a Ala Gly 130 Gly Tyr Giu Ph e Trp 210 Val1 Val1 Gi u Le u Met His Al a Pro 115 Ile Le u Se r Leu Pro 195 Ile Asp Leu Met Arg Pro Pro Asp 100 Asn Glu As n Gin Phe 180 Ile Arg Arg Gin Arg Ile Ly s Tyr Tyr Ly s Leu Phe Ala 165 Glu Val His Met Arg Arg Gin Thr Glu Gly Thr Phe Ala Gin Ala 70 Tyr Ala Gi y Pro Arg 150 Phe Val1 Le u Arg Leu 230 Asp Ser Val1 Lys Val1 Ala His 135 Tyr Val Leu Ile Leu 215 Val1 His Glu Thr Ala Val Ser 120 Glu Phe Cys C ys Gly 200 Val1 Thr 25 Asn Phe Val1 Gly Th r 105 Glu Gin Phe Leu Met 185 Th r Giu Asp Trp Val1 Ph e ValI 90 Gly Ala His Ala Pro 170 Val1 Glu Glu Asp Lys Ala Gly 75 Glu Gly As n Leu Arg 155 Gly Gin Phe Gi y Le u 235 Asp Gin Arg Asp Pro Trp Asp Ala Leu Arg Ile Lys Giu Lys Leu Gly Leu Met 110 Ser Val Gly Tyr Val Asp Met Phe Leu 160 Gly Thr Leu 175 Lys Val Pro 190 Gly Leu Val Asp Giu Lys Ala Val Lys 240 Phe Ile Val Asp Ala His Ala Gly Leu Asp Val Ala Arg Leu His Asn 245 250 255 <210> 275 <211> 1242 <212> DNA <213> Corynebacteriun giutarnicum <220> <221> COS <222> (101)..(1219) <223> RXS00948 WO 01/00805 <400> 275 acaccctcca aatgatctcq taaaacagta ttgaatttag PCTIIBOO/00926 gtacgactct aatcgtacct tgccctcaag ccaagctagt tgtacgatca aactcgttgt atg gca aac gtc gta cta Le u caa Gin t gc Cys cag Gin gtt Val1 ctt Leu gca Ala gct Al a gtt Val gca Al a 150 caa Gin gct Ala cat His gga Gly gtc Val1 atc Ile acc Thr gct Ala ga a Glu gga Gly gct Al a ggC Gly gga Gly 135 ttc Phe gag Giu gg t Gly aac Asn t Ct Se r ga t Asp cgc Arg tac Tyr cat His gca Ala Ct t Leu att Ile og c Ar g 120 cca Pro cca Pro gt t ValI ttt Ph e ttc Phe 200 tta Leu cga Arg gac Asp tct Se r tac Ty r act Th r tqg Trp cg c Ar g 105 aag Lys gaa Giu gg t Gly gt g Val1 gat Asp 185 ctt Leu ga a Glu atg Met atc Ile gca Ala g ca Ala gga Gly agc Ser gcc Ala qca Ala acc Thr ttg Le u ca g Gin 170 gt c Val1 tct Ser aac Asn gag Glu acc Thr g cc Ala gct Al a gtg Val1 cat His ggt Gly tcc Ser aa t As n cct Pro 155 ca g Gin gtg Val c cg Pro cgc Arg cct Pro atc Ile acc Thr cgc Arg 60 a ac Asn gac Asp ggg Gly acc Thr gga Gly 140 gct Ala ttc Ph e ga a Glu atc Ile gc t Ala t tg Le u ccc Pro gg t Gly 45 gc a Ala ccc Pro caa Gin gca Ala ga t Asp 125 t gg Trp ccg Pro gct Ala att Ile tcc Ser 205 cgc Arg gtg Val aac Asn 30 t Ca Ser gca Ala gt a Val1 att Ile gta Val1 110 gct Ala gag Giu cgc Arg gg9C Gi y ca c His 190 aac Asn atc Ile t cc Se r 15 cg c Arg ggt Gly ggt Gly gct Ala gaa Giu 95 ccg Pro ccg Pro act Th r gag Giu g cc Ala 175 gca Al a aag Lys gtg Val1 Met Ala ctg ttt Leu Phe tgg atg Trp Met ccc acc Pro Thr gtc gga Val Gly atc tcc Ile Ser ttc tcc Phe Ser gtt caa Val Gin aat ggt Asn Gly 130 ggc ccc Giy Pro 145 acg gtt Thr Val gtt cgt Val Arg cac ggc His Gly acc gat Thr Asp 210 gaa gtc Glu Val Asn Val Val acc Thr t ca Se r ga t Asp tta Leu cca Pro cg a Arg tta Leu 115 ggc Gly agc Ser tca Ser gcc Ala tac T yr 195 tca Se r att Ile cca Pro ccg Pro ttt Phe gtc Val gtc Val1 gtg Val 100 gcc Ala gg a Gly cct Pro gaa Glu ga t Asp 180 ctt Le u tac Tyr ga t Asp 163 211 259 307 355 403 451 499 547 595 643 691 739 787 wo 01/00805 WO 0100805PCT/IBOO/00926 atc Ile 230 acc Thr a cg Th r gt t Val1 ccg Pro agt Se r 310 act Th r ggc Gly gca Al a cgc gca Arg Ala acc gac Thr Asp ctg agc Leu Ser gat ttg Asp Leu 280 cat qac His Asp 295 acc gga Thr Gly gcg cac Ala His cgt cca Arg Pro cta ggt Leu Gly gtg Val1 tgg Trp caa Gin 265 atc Ile cqc Arg gtg Val aa t As n ctg Le u 345 gcg tgg T rp gtg Val 250 agc Ser ga t Asp ga t Asp a ca Thr t tg Leu 330 ctc Leu gaa cca Pro 235 gag Glu a gg Arg.

gcc Ala tac Tyr gtc Val1 315 gt t Val1 aag Lys ccc gag Glu qaa Glu ca g Gln t ct Se r caa Gin 300 gct Ala gat Asp ga t Asp agg gaa aag cct gta ttc atg cgc att tc Glu aac Asn ct g Leo tct Ser 285 acc Thr gct Ala tct Ser cc t Pro tat Tyr 365 Lys cca Pro gct Ala 270 ggt Gly gcq Ala gt g Val ggc Gly tcc Ser 350 gtt Val1 Met Arg Ile Ser tcc Ser 260 cac His ccc Pro cac His g cc Al a ttC Ph e 340 gcc Ala gta Val1 tgg Trp gga Gly att Ile gca Ala ca a Gin 325 ct C Leu ctc Leu ctt Leu 883 931 979 1027 1075 1123 1171 1219 1242 Ala Glu Pro Arg 360 taaaggagag ttgacatgaa ggt <210> 276 <211> 373 <212> PRT <213> Corynebacterirn glotamicom <400> 276 Met Ala Asn Val Val Leo Val Asp i 1 5 Leo Phe Thr Pro Ile Gln Ile Arg 1 Trp Met Ser Pro Met Cys Thr Tyr 40 Pro Thr Asp Phe His Gln Ala His 55 Val Gly Leu Val Met Val Glu Ala 70 Met 10 Ile Ala Al a Gb y Glu Pro Leu Val Ser Lys Thr Ile Pro Asn Arg Val Ala Thr Gly Ser Gly Leo Ala Arg Ala Ala Gly Gly Val Asn Pro Val Ala Pro 75 WO 01/00805 PCT/IB00/00926 Ile Ser Pro Val Asp Leu Gly Leu Trp Ser His Asp Gin Ile Glu Pro 90 Phe Ser Arg Val Thr Ala Ala Ile Arg Ala Gly Gly Ala Val Pro Ala 100 105 110 Val Gin Leu Ala His Ala Gly Arg Lys Ala Ser Thr Asp Ala Pro Trp 115 120 125 Asn Gly Gly Gly Tyr Val Gly Pro Glu Thr Asn Gly Trp Glu Thr Val 130 135 140 Gly Pro Ser Pro Leu Ala Phe Pro Gly Leu Pro Ala Pro Arg Glu Leu 145 150 155 160 Thr Val Ser Glu Ile Gin Glu Val Val Gin Gin Phe Ala Gly Ala Ala 165 170 175 Val Arg Ala Asp Gin Ala Gly Phe Asp Val Val Glu Ile His Ala Ala 180 185 190 His Gly Tyr Leu Leu His Asn Phe Leu Ser Pro Ile Ser Asn Lys Arg 195 200 205 Thr Asp Ser Tyr Gly Gly Ser Leu Glu Asn Arg Ala Arg Ile Val Leu 210 215 220 Glu Val Ile Asp Ala Ile Arg Ala Val Trp Pro Glu Glu Lys Pro Val 225 230 235 240 Phe Met Arg Ile Ser Thr Thr Asp Trp Val Glu Glu Asn Pro Gin Asp 245 250 255 Asp Arg Glu Ser Trp Thr Leu Ser Gin Ser Arg Gin Leu Ala Leu Trp 260 265 270 Ala Ser Glu His Gly Val Asp Leu Ile Asp Ala Ser Ser Gly Gly Leu 275 280 285 Asp Ile Val Pro Ile Pro His Asp Arg Asp Tyr Gin Thr Ala Lys Ala 290 295 300 Ala Asp Leu His Ala Ser Thr Gly Val Thr Val Ala Ala Val Gly Arg 305 310 315 320 Ile Asp Asp Ala Gin Thr Ala His Asn Leu Val Asp Ser Gly Asp Val 325 330 335 Asn Ala Val Phe Leu Gly Arg Pro Leu Leu Lys Asp Pro Ser Trp Ala 340 345 350 Asn Gin Ala Ala Leu Ala Leu Gly Ala Glu Pro Arg Tyr Val His Gin 355 360 365 Tyr Asp Tyr Val Leu 370 <210> 277 <211> 1026 WO 01/00805 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1003) <223> RXS02228 <400> 277 PCTJIBOO/00926 aoagaagcgt caactgctca acttotcgao agcgacgaca cacacggcgt gggacgctac gttgctcgct ggacttttta aottaaggga gatctagatc gtg gta aca ccg ato 11 Val Val Thr Pro Ile g ca Ala cta Leu ctg Leu cgc Arg act Th r ga t Asp ttg Leu agc Ser ga a Giu gtc Val1 150 att Ile gcg Ala gga Gly aa g Lys gg c Gi y att Ile gt g Val1 tcc Ser caa Gin 105 cgc Arg cac His agc Ser acc Th r 090 Arg ccc Pro Ott Leu a tg Met g cg Ala 9 cg Ala cgt Arg tot Ser g ca Ala got Ala aat As n ggo Gly 170 tgg Trp gca Ala ggt Gly ato Ile oat His ttt Phe aaa Lys gtc Val1 ttt Phe ott Le u 140 000 Pro 000 Pro act Thr tot Se r ga a Giu gg 0 Gly cag Gin oaa Gin a 00 Thr ga t Asp gag Giu 125 act Thr oga Arg tto Phe oga Arg gga Gly gt a Val1 aog Thr ctc Le u too S er coo Pro ga t Asp 110 goo Al a oa g Gin ogo Arg caa Gin ato Ile aaa Lys gt o ValI goa Ala ga t Asp gac Asp atc Ile tgg Trp ogo Arg ott Le u aoo Thr goa Ala 175 att Ile 1 got Ala gtg Val1 otg Le u tgg Trp gtt Val1 gtt Val1 tto Phe goa Al a oca Pro 145 og a Arg oaa Gin ctg Le u ttg Leu ga t Asp act Thr gao Asp g 00 Al a ggo Gly o oa Pro gao Asp 130 gaa Giu goa Ala cog Pro aaa Lys got Al a ca g Gin gaa Giu ga a Giu gaa Giu a tg Met gac Asp gtc Val1 g 00 Ala gto Vai 165 gao Asp ooa Pro WO 01/00805 185 gaa tgg cta aat coa cgc ato gag Glu Trp Leu Asn Pro Arg Ile Giu 200 205 caa ggc ttt gtc gcc gaa gtg gaa Gin Giy Phe Val Ala Glu Val Glu 215 .220 got gao tcc aco gcg gga cga gca Ala Asp Ser Thr Ala Giy Arg Ala 230 235 gcc atg gca ggg gag atg acc tgg Ala Met Ala Gly Glu Met Thr Trp 250 acc gga acc aga cgc tat gtc agg Thr Gly Thr Arg Arg Tyr Val Arg 265 gao cac cgc gtg too tgg gto gao Asp His Arg Val Ser Trp Val Asp 280 285 goc ttg gag att ttg ggt ota caa Ala Leu Giu Ile Leu Gly Leu Gin 295 300 <210> 278 <211> 301 <212> PRT <213> Corynebacterium giutarnicum <400> 278 Val Val Thr Pro Ile Ala Val Val 1 5 Ala Leu Gly Ile Ala Leu Ala His Val Asp Ser Met Gin Leu Tyr Lys Leu Thr Val Giu Giu Arg Glu Gly Trp Asp Val Thr Glu Thr Ala Ser 70 Val Ala Asp Val Giu Asp Ile Met Val Gly Gly Ser Met Leu Tyr Val 100 Phe Pro Pro Thr Asp Ser Ala Val 115 120 PCTIBOOIOO926 190 195 cag ogo aco goc agg atg ttt Gin Arg Thr Ala Arg Met Phe 210 cac ott gtg cag caa gga oto His Leu Val Gin Gin Gly Leu 225 atc ggc tao toc caa gta otg Ile Gly Tyr Ser Gin Val Leu 240 gaa gao goc ttc gaa cqc aog Glu Asp Ala Phe Glu Arg Thr 255 260 ogo oaa ogo ago tgg tto aao Arg Gin Arg Ser Trp Phe Asn 270 275 goc tot ggo gat 000 aoo goa Ala Ser Gly Asp Pro Thr Ala 290 tagogagggt gaatt.tgaoo ato gaa Giu ato Ile g oa Ala 245 gt 0 Val1 aga Arg oaa Gin Ser As n Lys Val Ala Le u Gin Le u 1026 Gly Lys Gly Ile Val1 Ser Gin 105 Arg Lys Val Ala Asp Asp Ile Trp Arg WO 01/00805 PCTABOO/00926 Ala Asp 130 Pro Giu 145 Arg Ala Gin Pro Leu Lys Ala Arg 210 Gin Gin 225 Ser Gin Phe Giu Ser Trp Asp Pro 290 Ile Gly Val Giu Ala Leu His Ala Giu Leu Thr Gin Leu Asp Ala Le u Pro Th r 195 Met Giy Val Arg Phe 275 Thr Al a Giu Lys 180 Thr Ph e Leu Le u Thr 260 As n Ala Ala Val1 165 Asp Pro Giu Ile Ala 245 Val1 Arg Gin Val1 150 Ile Ala Glu Gin Ala 230 Ala Th r Asp Ala As n Gi y 170 Trp Pro Al a Al a Glu 250 Ar q Ser Le u Asp 155 Gin Gi y Arg Glu Gly 235 Met T yr Trp Gly 140 Pro Pro Thr Ile Val1 220 Arg Th r Val1 Val1 Leu 300 Arg Gin Ile 190 Gin His Ile Giu Arg 270 Ala <210> 279 <211> 954 <212> DNA <213> Corynebacterium giutanicun <220> <221> CDS <222> (931) <223> RXC01971 <400> 279 aggtcttgtt tatttcggct actgattcag tagctgcgct cagttcagta tctttgagcc acggctagaa tgtgaatcct cct cgc ccc att ccg gtt cct gcc Pro Arg Pro Ile Pro Val Pro Ala gcg cat aca cat ttq gca tcg tgt Ala His Thr His Leu Ala Ser Cys gaa agg gcc aag gag qcg ggc gtc Giu Arg Ala Lys Giu Ala Gly Val ttt atc Phe Ile 15 gga gat Gly Asp aag ctt Lys Leu ccgataggat icttagittt atg tct aag aag aag 115 Met Ser Lys Lys Lys 1 cct ggi ctc att gat 163 Pro Gly Leu Ile Asp ctt gca ggg ttg gig 211 Leu Ala Gly Leu Val tgt acc gtc ggt gat 259 Cys Thr Val Gly Asp WO 01/00805 PTTOIO2 PCTfEBOO/00926 ggt Gly aat Asn ga t Asp ig t Cys ga t Asp igg T rp aat Asn 150 cct Pro gog Ala gtg ValI ccg Pro gag Glu 230 g cg Al a gc g ttg Leu gtg Val1 ggg Gly gtg Val1 cca Pro cat His 135 cq t Arg cca Pro aag Lys a cg Thr at Ile 215 ccg Pro cia Le u gc t gci Al a ttt Ph e gct Al a gcc Ala gag Glu 120 ati Ile gag Glu aaa Lys gaa Glu ttt Phe 200 tcc Ser ttt Phe tgC Cys ttg gag Glu gct Ala g cg Ala ati Ile 105 ga c Asp gati Asp gcg Al a gati Asp gcg Al a 185 aag Lys ca g Gln Arg ati Ile aat gcc Ala gcg Ala cg t Arg ggt Gly a cg Thr tig Leu gat Asp a cg Th r 170 tig Le u cgt Arg ati Ile ggg Gly g cg Al a 250 gag Giu ctt Leu 60 g cg Ala cgg Arg act Thr g cg Ala at Ile 140 gat Asp cig Leu cgt Arg gag G iu at Ile 220 a gg Arg gt Val1 iii gcg Ala ati Ile ctg Leu ggt Gly t ig Leu 125 agi Ser tig Leu cat His gga Gly gag Giu 205 gaa Glu aat Asn cgg Arg cig Leu cat His a cg Thr ttg Leu 110 gat Asp gcg Al a a tg Met igi Cys tat Tyr 190 t ig Le u acc Thr gag Glu ggg Gi y gcc Ala a cg Thr 80 atg Met i cg Se r caa Gin aag Lys gig Val1 160 t ci Se r t ig Leu gag Glu gcg Ala i cg Se r 240 gci Ala g cg Ala aag Lys gcg Ala tat Tyr gag Glu c cq Pro 145 tig Le u t cg Ser agi Ser gci Ala ccg Pro 225 tg Leu gt g Val1 caa Gin gct Ala gcg Ala tgg Trp gag Glu 130 tig Leu gcg Ala ccg Pro ttt Ph e gci Ala 210 tat Tyr at Ile gag Glu cag ttt ggc Gin Phe Gly gai cag tig Asp Gin Leu gat ccg aai Asp Pro Asn 100 aic aag cac Ile Lys His 115 gcg cig cgc Ala Leu Arg aig ait cac Met Ile His gat gct cca Asp Ala Pro 165 tig qac gig Leu Asp Val 180 gcg ggc aai Ala Gly Asn 195 cgt ait gcg Arg Ile Ala aig acg ccg Met Thr Pro ggi cat acg Gly His Thr 245 gat git gcg Asp Val Ala 260 aca aat cia Thr Asn Leu aat Ala Ala Leu Asn Asn Phe gat cgc gti tat ggg gtc Asp Arg Val Tyr Gly Val 270 iaacgtgagg iagctcacag ica WO 01/00805 <210> 280 <211> 277 <212> PRT <213> Corynebacterium glutamicum <400> 280 Met Ser Lys Lys Lys Pro Arg Pro Ile PCT/IBOO/00926 1 Pro Le u Cys Al a Lys Al a Tyr Glu Pro 145 Leu Ser Ser Ala' Pro 225 Le u Val Gl y Ala Thr Gin Ala Ala Trp Glu 130 Leu Al a Pro Phe Al a 210 Tyr Ile Glu Leu Ile Giy Leu Val Gly Gin Phe Asp Gln Asp Pro 100 Ile Lys 115 Ala Leu Met Ile Asp Ala Leu Asp 180 Ala Gly 195 Arg Ile Met Thr Gly His Asp Val 260 5 Asp Val Asp Gly Le u Asn His Arg His Pro 165 Val1 Asn Ala Pro Thr 245 Ala Ala Giu Gly As n 70 Asp Cys Asp Trp Asn 150 Pro Ala Val Pro Glu 230 Ala Ala His Arg Le u 55 Val1 Gly Val Pro His 135 Ar q Pro Lys Th r Ile 215 Pro Le u Ala Th r Ala 40 Ala Phe Ala Ala Glu 120 Ile Giu Lys Giu Phe 200 Ser Phe C ys Leu His 25 Lys Glu Ala Ala Ile 105 Asp Asp Ala Asp Ala 185 Lys Gin Arg Ile As n 265 Pro 10 Leu Glu Ala Ala Arg 90 Gly Th r Leu Asp Thr 170 Leu Arg Ile Gly Al a 250 Glu Val1 Al a Ala Glu Cys 75 Ala Glu Ala Ala Al a 155 Ile Asp As n Leu Ser 235 Glu Asn Pro Ser Gly Leu Ala Arg Thr Ala Ile 140 Asp Leu Arg Gin Ile 220 Arg Val1 Ph e Ala Cys Val1 Ala Ile Leu Gly Leu 125 S er Leu His Gly Glu 205 Gin Asn Arg Asp Gin Gly Giu Len His Th r Len 110 Asp Ala Met Cys Tyr 190 Le u Th r Giu Gly Arg 270 Phe Gly Lys Giu Pro Gln Asp Val1 Asp Arg Phe 175 Val Arg Asp Pro Met 255 Val1 Ile Asp Len Ala Thr Met Ser Gin Lys Val1 160 Se r Len Giu Ala Ser 240 Ala Tyr Gly Val Thr Asn Leu 275 WO 01/00805 <210> 281 <211> 1650 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1627) <223> RXC02697 <400> 281 tttggetcac ctcgatgatg tagacatccc egatgaggtg ggctatecge tcaacegaac gtcggatgta gtagacgcgt PCTLBOOIOO926 cgcgcacagt tgcgggcact aca ctt ttt caa Thr Leu Phe Gin cg t Arg ct g Le u etc Le u att Ile tgg Trp acc Thr a tg Met tat Tyr tac Tyr t ct Ser 150 t gg Trp tta Leu etc Leu ga t Asp tcc Ser gtt Val gca Aid gtg Val1 tee Ser act Th r 135 cat His att Ile ace Th r gge Gly geg Ala aat Asn cac His gac Asp gt g ValI tat Tyr 120 gag Glu ga t Asp gga Gly aae Asn tet Ser t tg Le u ace Thr ctt Leu get Ala ec Pro 105 etc Le u gge Gi y t gg Trp ga e Asp ct Pro ttc Phe ggg Gly gtg Val1 gga Gly get Ala etc Leu atg Met get Aila eg e Arg a tg Met 170 gta Val1 ggt Gi y ctt Leu ttg Leu egt Arg 75 gac Asp att Ile cag Gin g eg Ala aac As n 155 ate Ile etc Leu ggt Gly tte Ph e gtt Val1 ttg L eu ggt Gly g eg Ala geg Ala 125 aac Asn a eg Th r ac g Th r gge Gly gee Ala ett Leu ggg Gly ttc Ph e ctt Leu gea Al a 110 atg Met cet Pro a cg Thr gtt Val 1 ggc eta gca Gly Leu Ala 15 att egg tac Ile Arg Tyr get ttt ggc Ala Phe Gly eag ctg etg Gin Leu Leu aag aaa aaa Lys Lys Lys 80 gta aag ege Val Lys Arg 95 eca atg atg Pro Met Met etg egt gat Leu Arg Asp ggc ccc atg Giy Pro Met 145 ceg tat ggt Pro Tyr Gly 160 gtg ggc gat Val Gly' Asp 175 ttg Leu g ga Gly ca g Gin Gly gat Asp tat Tyr 100 ga t Asp ga t Asp gag Glu cac His ctt Leu gtg Val ggt Gly geg Ala ga e Asp gee Ala gt t Val ccc Pro gte Val etg Leu 165 115 163 211 259 307 355 403 451 499 547 595 643 aat gte ace ttg Asn Val Thr Leu 180 WO 01/00805 WO 0100805PCT/I.B00/00926 99 c Gly ggc Gly att Ile atc Ile 230 ggc Gly att Ile ttt Phe aaa Ly s ttt Phe 310 9e9 Ala ag t Ser gcc Ala tac Tyr ctt Leu 390 agg Arg aat gtC Val1 tgg Trp gca Ala 215 9gC Gly ttg Le u gca Ala qtg Val a ag L ys 295 gcc Ala atc Ile Gi y agt Ser aac Asn 375 9gc Gly 9c9 Al a tct ttg Le u gca Al a 205 gcc Al a tcc Ser a ag Lys aaa Lys cat His 285 acc Thr qt t Val1 gct Ala aac As n gct Ala 365 gtg Val1 t gg Trp qc9 Ala tac t cg Ser 190 ca a Gin aat Asn ct C Leu cg t Arg gct Al a 270 cat His ctt Le u gc t Ala tcg Ser ccg Pro 350 gaa Glu cg C Arg tgg Trp gc t Ala tac atc Ile cat His cc t Pro atg Met ttc Phe 255 aca Thr ttc Ph e aag Lys gtc Val1 t g9 T rp 335 Ct 9 Leu gtg Val1 t ca Ser ctg Leu tat T yr 415 gcC att Ile ttt Phe gtg Val1 gtq Val1 240 gtg Val1 g9cg Ala g ca Ala ca a Gin act Thr 320 9gC Gly gc t Al a ttc Ph e atc Ile ttc Phe 400 gc c Ala a gc ggc Gly gga Gly atg Met 225 gga Gly gca Al a 9c9 Ala gga Gly ca q Gin 305 ggt Gly t 99 Trp ttc Phe gt t Val1 tcc Se r 385 cg c Arg gtg Val1 ttq ctt Le u gcc Al a 210 atc Ile ttg Leu ggt Gly att Ile ttc Ph e 290 qt c Val1 gtt Val atc Ile cct Pro gac Asp 370 atg Met cag Gin gc t Al a atc a ca Th r gca Al a atg Met gc Val 245 ct c Leu cct Pro acc Thr ttc Phe agt Se r 325 atc Ile gtg Val1 gac Asp atg Met cgc Arg 405 ttc Phe ctc 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 WO 01/00805 PCT/IBOO/00926 Asn Ser Val Thr Leu Pro Trp Tyr Tyr Ala Ser Leu Ile Ser Leu Leu 425 430 435 ggc aca itt aaa cca ccg atg tgg itg att cgc itc gca gcg ggt gci 1459 Gly Thr Phe Lys Pro Pro Met Trp Leu Ile Arg Phe Ala Ala Giy Ala 440 445 450 icg gig tit atc gcg ctg aig itt acc gga agt gga aac cac cag ctg 1507 Ser Val Phe Ile Ala Leu Met Phe Thr Gly Ser Gly Asn His Gin Leu 455 460 465 tac aac atc gtt acg gtg atc atc gca gca att atc'gcg tgg ctt gcc 1555 Tyr Asn Ilie Val Thr Val Ile Ile Ala Ala Ile Ile Ala Trp Leu Ala 470 475 480 485 acc gtg gig atc itt gat gac act gac cci gca aca acg gcc acg gag 1603 Thr Val Vai Ile Phe Asp Asp Thr Asp Pro Ala Thr Thr Ala Thr Glu 490 495 500 aaa ccc icc ccg cat acc gtt icc tagttgcata aqgtaaaccg cca 1650 Lys Pro Ser Pro His Thr Val Ser 505 <210> 282 <211> 509 <212> PRT <213> Corynebacterium glutamicum <400> 282 Met Thr Leu Phe Gin Arg Leu Thr Asn Pro Val Val Leu Gly Giy Leu 1 5 10 Ala Gly Vai Leu Leu Leu Leu Gly Ser Phe Gly Gly Gly Ala Ile Arg 25 Tyr Arg Gly Giy Vai Leu Asp Ala Leu Gly Leu Asn Phe Leu Ala Phe 40 Gly His Aia Gin Gly Ile Ser Asn Thr Val Leu Trp Val Gly Gin Leu 55 Leu Leu Ile Gly Ala Trp Val His Leu Gly Arg Arg Leu Phe Lys Lys 70 75 Lys Val Ala Asp Asp Thr Ala Asp Ala Ala Asp Leu Gly Leu Val Lys 90 Arg Thr Leu Tyr Ala Met Val Val Pro Leu Ile Phe Ala Ala Pro Met 100 105 110 Met Ser Arg Asp Val Tyr Ser Tyr Leu Met Gin Gly Ala Met Leu Arg 115 120 125 Asp Gly Phe Asp Pro Tyr Thr Glu Gly Ala Ala Val Asn Pro Gly Pro 130 135 140 Met Leu Leu Giu Val Ser His Asp Trp Arg Asn Thr Thr Thr Pro Tyr 145 150 155 160 Gly Pro Leu His Leu Trp Ile Gly Asp Met Ile Thr Thr Val Val Gly WO 01/00805 WO 0100805PCTJIBOO/00926 Asp Gly Gly Met 225 Gly Ala Ala Gly Gin 305 Gly Trp Phe Val1 Ser 385 Arg Val1 Le u Ph e Gly 465 Ile As n Leu Ala 210 Ile Le u Gly Ile Phe 290 Val1 Vali Ile Pro Asp 370 Met Gin Ala Ile Ala 450 Asn Ala Val Ala 195 As n Ile Val1 Val Ala 275 Leu Pro Val1 Se r Ser 355 Asp Leu Asn Ph e Se r 435 Ala His Trp Th r 180 Val1 Pro His Ser Ala 260 Leu Ala Ala Val1 Giu 340 Le u Ph e Ile Glu Val1 420 Le u Gly Gin Leu 165 Leu Thr Ala Met Val1 245 Le u Pro Thr Phe Ser 325 Ile Val1 Asp Met Arg 405 Phe Leu Ala Leu Ala 485 Gly Gly Ile Ile 230 Gly Ile Ph e Lys Ph e 310 Ala Se r Ala Tyr Leu 390 Arg As n Gly Ser Tyr 470 Th r Val1 Trp Al a 215 Gly Leu Ala Val1 Lys 295 Ala Ile Gly Ser Asn 375 Gly Ala Ser Thr Val 455 Asn Val Val Ser 200 Leu Gly Leu Val1 Val1 280 Gly Thr Thr As n Val1 360 Al a Gly Val1 Val1 Phe 440 Phe Ile Val1 Ala 185 Ile Trp Met Leu Al a 265 Trp Lys Gly Trp Ser 345 Ile Val Le u Th r Th r 425 Lys Ile Val Ile 170 Tyr Val1 Ile His Al a 250 Val1 Ile Asp Ala Ala 330 L ys Thr Val1 Val1 Gly 410 Leu Pro Al a Thr Ph e 490 Lys Arg Gly As n 235 Le u Ser Gly Se r Al a 315 Se r Val Met Asn Val 395 Th r Pro Pro Leu Val 475 Asp Ile Ile Val1 220 Giu Lys Leu Met Pro 300 Gly Gly Ile Val1 Val 380 Cys Ala T rp Met Met 460 Ile Asp Leu Ala 205 Ala Ser Lys Lys His 285 Thr Val Ala As n Ala 365 Val Trp Al a Tyr Trp 445 Phe Ile Th r Ser 190 Gin Asn Leu Arg Ala 270 His Leu Ala Ser Pro 350 Glu Arg T rp Ala Tyr 430 Leu Th r Ala Asp 175 Ile His Pro Met Phe 255 Thr Phe Lys Val1 T rp 335 Leu Val1 Ser Le u Tyr 415 Ala Ile Gi y Ala Pro 4 Ile Phe Val1 Val 240 Val Al a Al a Gin Thr 320 Gly Al a Ph e Ile Phe- 400 Ala Ser Arg Ser Ile 480 Ala WO 01/00805 Thr Thr Ala Thr Glu Lys Pro Ser Pro His Thr Val Ser 500 505 <210> 283 <211> 1298 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(1275) <223> RXN01946 PCTIBOOIOO926 <400> 283 atc cgc az Ile Arg L 1 tac gaa gc Tyr Glu A] gag gca cc Giu Ala Ai cgc cgc cc.

Arg Arg Ai tcc ggc a Ser Gly L ttg gac 9g Leu Asp A' gaa ggt gc Glu Gly G1 gta tgt ai Val Cys A., 1: gtc tac ai Val Tyr A.- 130 gaa gca c( Glu Ala Aj 145 gcc ctc aj Ala Leu L, gcc gca gc tac Tyr gac Asp atc Ile gtC Val tca Ser gac Asp ctg Leu 100 aag Lys atg Met cga Arg ga c Asp aa g agg Arg ga a Glu ga c Asp ttg Leu ac Thr 70 atc Ile atg Met tgg Trp ttt Phe cg t Arg 150 gct Ala atg ctc Leu gca Ala ca g Gin gcg Al a aca Thr acc Th r att Ile tac Tyr agc Ser 135 gag Giu gca Ala atc ctc Le u ct C Leu qgc Gly acc Th r acc Thr gcg Ala ctt Leu 110 gaa Giu g ca Ala a ag L ys acc Th r ggc Gi y gg C Gly ggc Gly aac Asn aac As n aag Lys ggc Gly gcc Ala CtC Leu gcc Al a aag Lys ggc Gi y ctc Leu ca g Gin ggc Gly ca C His cac His gcc Al a ga t Asp ga t Asp gga Gly 160 gct Ala 175 gaa cga atg atc gac aac WO 01/00805 WO 0100805PCT/IBOO/00926 Ala Ala Ala Lys Gin Met Ile Ala Arg Ala ga c Asp cc a Pro 210 atg Met aaa Lys acc Th r ggo Gly cac His 290 gc Ala ttc Phe ggc Gly gca Ala too Se r 370 gtc Val1 cgc Arg tac Tyr gaa Glu 195 g ca Ala tac Tyr 99C Gly ctg Leu atc Ile 275 gao Asp tgo Cys atg Met ggt Gly aac Asn 355 090 Arg ctg Leu gtc Val1 atg Met gct Al a aaa Lys 215 ga a Glu gt c Val1 ctc Le u tao Tyr ccc Pro 295 gao Asp ga a Glu cgc Arg otc Le u ct 0 Leu 375 gac Asp Oct Pro ga a Glu gac Asp 200 a cc Th r gtc Val gtc Val1 gcc Ala qgo Gly 280 gac Asp caa Gin caa Gin ctc Le u ga c Asp 360 gac Asp ccg Pro ga t Asp ttg Leu 185 cgc gcc Arg Ala cca ctc Pro Leu ttc gcc Phe Ala ctc gqa Leu Gly 250 ggt gtg Gly Val 265 ctc aaa Leu Lys aaa too Lys Ser caa agc Gin Ser ctc gao Leu Asp 330 gca ctg Ala Leu 345 gag ccc Glu Pro gca ctg Ala Leu ggt goa Gly Ala ggc acc Gly Thr 410 gcg tagc Ala Glu gc Ala aac As n ggc Gly 235 ttc Phe ga a Glu atc Ile gtc Val ctc Leu 315 caa Gin gcc Al a acc Thr cgc Arg gtc Val 395 gag Arg aac Asn gc Al a 220 gtc Val1 aac As n cg c Arg ggc Gly t gg Trp 300 cgo Arg cca Pro acc Thr a ac Asn acc Thr 380 aag Lys gat Met atc Ile 205 aag Lys ga c Asp gg t Gly acc Th r tac Tyr 285 caa Gin ago Ser go a Al a ot 0 Leu aao Asn 365 tao Tyr gc Al a ott Asp tto Phe ct g Le u gc Ala gg t Gi y 255 ggc Gly gc Al a acc Th r ot 0 Leu aca Th r 335 too Ser gao Asp ggc Gi y gag Glu aat Asn o ca Pro aco Thr atc Ile 240 aaa Lys gaa G lu cag Gin ato Ile gga Gly 320 Ct 0 Le u too Se r cg Pro gca Al a cca Pro 400 ga t Asp 624 672 720 768 816 864 912 960 1008 1056 1104 1152 1200 1248 1295 Glu Asp Leu Trp Asn 415 WO 01/00805 PCT/IB00/00926 420 425 gct 1298 <210> 284 <211> 425 <212> PRT <213> Corynebacterium glutamicum <400> 284 Ile Arg Lys Tyr Ser Arg Leu Glu Glu Gin Phe Gin Ser Leu Gly Gly 1 5 10 Tyr Glu Ala Asp Ala Glu Ala Ala Gin Ile Cys Asp Asn Leu Gly Leu 25 Glu Ala Arg Ile Leu Asp Gin Gin Leu Lys Thr Leu Ser Gly Gly Gin 40 Arg Arg Arg Val Glu Leu Ala Gin Ile Leu Phe Ala Ala Thr Asn Gly 55 Ser Gly Lys Ser Lys Thr Thr Leu Leu Leu Asp Glu Pro Thr Asn His 70 75 Leu Asp Ala Asp Ser Ile Thr Trp Leu Arg Asp Phe Leu Ala Lys His 90 Glu Gly Gly Leu Ile Met Ile Ser His Asp Val Glu Leu Leu Gly Ala 100 105 110 Val Cys Asn Lys Ile Trp Tyr Leu Asp Ala Val Arg Ser Glu Ala Asp 115 120 125 Val Tyr Asn Met Gly Phe Ser Lys Tyr Val Asp Ala Arg Ala Leu Asp 130 135 140 Glu Ala Arg Arg Arg Arg Glu Arg Ala Asn Ala Glu Lys Lys Ala Gly 145 150 155 160 Ala Leu Lys Asp Gin Ala Ala Arg Leu Gly Ala Lys Ala Thr Lys Ala 165 170 175 Ala Ala Ala Lys Gin Met Ile Ala Arg Ala Glu Arg Met Ile Asp Asn 180 185 190 Leu Asp Glu Ile Arg Val Ala Asp Arg Ala Ala Asn Ile Val Phe Pro 195 200 205 Glu Pro Ala Pro Cys Gly Lys Thr Pro Leu Asn Ala Lys Gly Leu Thr 210 215 220 Lys Met Tyr Gly Ser Leu Glu Val Phe Ala Gly Val Asp Leu Ala Ile 225 230 235 240 Asp Lys Gly Ser Arg Val Val Val Leu Gly Phe Asn Gly Ala Gly Lys 245 250 255 Thr Thr Leu Leu Lys Leu Leu Ala Gly Val Glu Arg Thr Asp Gly Glu 260 265 270 WO 01/00805 WO 0100805PCTJIBOOIOO926 Gly Giu Giu 305 Ser Se r Arg Ile Val1 385 Giu Gin Giy His 290 Ala Ph e Giy Aia Ser 370 Val1 Arg Tyr Ile Val Thr Gly Tyr 275 Asp Thr Cys Ala Met Phe Gly Giu 340 Asn Vai 355 Arg Giu Leu Vai Vai Ile Met Giu 420 Asp Aia 310 Gly Thr Le u Val His 390 Le u Val1 Pro 295 Asp Giu Arg Le u Leu 375 Asp Pro Glu Gly 280 Asp Gin Gin Leu Asp 360 Asp Pro Asp Leu Leu Lys Gin Leu Ala 345 Giu Ala Gly Gly Aia 425 Lys Ser Ser Asp 330 Leu Pro Le u Ala Thr 410 Al a Thr Leu Th r 335 Ser Asp Gi y Gi u Asn 415 Gin Ile Giy 320 Leu Ser Pro Ala Pro 400 Asp <210> 285 <211> 1298 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(1275) <223> FRXA01946 <400> 285 atc cgo aag tac too agg oto gag Ile Arg Lys Tyr Ser Arg Leu Glu 1 tac gaa gct gac goc gaa gca gcc Tyr Giu Ala Asp Ala Giu Ala Ala gaq gca cgc atc oto gao cag cag Giu Ala Arg Ile Leu Asp Gln Gin 40 cqc cgc cgc gtc gag ttg gcg cag Arq Arq Arg Val Giu Leu Aia Gin 55 tcc ggc aaa tca aaa acc aca ttg Ser Gly Lys Ser Lys Thr Thr Leu I cag Gin gac Asp ct g Le u gco Ala gag Glu tog Ser aao As n too Ser gc Ala 000 Pro WO 01/00805 WOOI/0805PCTIBOOOO926 ttg Le u ga a Glu gta ValI gtc Val1 qa a Glu 145 gcc Al a qcc Al a ctc Leu qa a Glu aaq Lys 225 qa c Asp acc Thr ggc Gly gaa Glu gaa Glu 305 gac Asp ggt Gly tgt Cys tac Tyr 130 qca Ala ctc Leu 9 ca Ala qac Asp cca Pro 210 atg Met aaa Lys acc Th r ggc Gly cac His 290 gcc Al a gca Ala gga Gly aa c Asn *115 aac Asn cgc Arg aag Lys gc t Ala gaa Glu 195 gca Ala tac Tyr qqc Gly ctg Leu atc Ile 275 gac Asp t gc C ys gac Asp ctg Leo 100 aag Lysatg Met cga Arg ga c Asp aag Ly s 180 atc Ile ccc Pro gqc Gly tcc Ser ctc Leo 260 gt c Val a cc Th r gcc Ala t c9 Ser atc Ile att Ile Gly cgc Arg ca g Gin 165 ca g Gin cgc Arq tqt Cys t cc Ser cg c Arg 245 aaa Lys a cc Thr atc Ile gac Asp atc Ile atg Met tgg Trp ttt Phe cg t Arg 150 gct Ala a tg Met gta Val1 gga Gly ctc Leu 230 gta Val1 Ct C Leu qqa Gly gac Asp gcc Ala 310 a cc Thr att Ile tac Tyr agc Ser 135 gaq Glu qca Ala atc Ile gct Ala aaa Lys 215 qa a Glu gtc Val1 ctc Leo tac Tyr ccc Pro 295 gac Asp tgg T rp t cg Ser ctc Leo 120 aaa Lys cgc Arq cqc Arg gcc Al a gac Asp 200 a cc Th r gtc Val1 gt c Val1 9cc Al a ggc Gly 280 gac Asp ca a Gin cic Leu cac His 105 gac Asp tac Tyr gca Ala ctc Leo cg t Arg 185 cqc Arq cca Pro ttc Phe ct c Leo 99 t Gly 265 ct c Le u aaa Ly s caa Gin Cgt Arg 90 ga c Asp gca Al a gt c Val1 aac As n ggc Gi y 170 qcg Ala qcc Ala ctc Leu gcc Ala gga Gly 250 gtg Val aaa Lys tcc Ser a gc Ser ga c Asp gt c Val1 gt a Val1 ga t Asp gcc Ala 155 gcg Al a gaa Gi u gcc Ala aac As n ggc Gly 235 ttc Phe ga a Glu atc Ile gtc Val1 ctc Leu 315 ttc Phe gaa Glu cgc Arg gca Ala 140 gaa Glu aaa L ys cga Arg aac Asn gcc Ala 220 gtc Val1 aac Asn cqc Arq Gi y tgg Trp 300 cgc Arg ctg Leo ctg Leu agc Se r 125 cg t Arq aag Ly s gca Ala atg Met atc Ile 205 aag Lys qac Asp ggt Gly acc Thr tac T yr 285 caa Gin agc Ser gCg Ala ctt Leu 110 gaa Glu qca Ala aag Lys acc Thr atc Ile 190 gt t Val1 Gi y ct a Leu gca Ala gac Asp 270 ttc Phe aac Asn ctc Leo aag Lys 99 C Gly gcc Ala ct c Leo gcc Ala aag Lys 175 gac Asp ttc Phe Ct 9 Leo gCC Ala ggt Gly 255 99C Gly gCC Ala acc Thr ct C Le u Cac His 9CC Ala gat Asp ga t Asp 9 qa Gly 160 gct Ala aa c Asn cca Pro a cc Thr a tc Ile 240 aaa Lys ga a Glu ca g Gin atc Ile gga Gly 320 288 336 384 432 480 528 576 624 672 720 768 816 864 912 960 WO 01/00805 tcc ttc atg ttc tcc ggc gaa caa ctc Ser Phe Met Phe Ser Gly Glu Gin Leu 325 tcc ggc ggt qaa aaa acc cgc ctc gca Ser Gly Gly Giu Lys Thr Arg Leu Ala 340 345 cgc gca aac gtc ctg ctt ctc gac gag Arg Ala Asn Val Leu Leu Leu Asp Giu 355 360 atc tcc cgc gaa cag gtc ctc gac gca Ilie Ser Arg Glu Gin Val Leu Asp Ala 370 375 gtc gtc ctg gtt acc cac gac ccg ggt Val Val Leu Val Thr His Asp Pro Gly 385 390 gaa cgc gtc atc gtg ctt cct gat ggc Giu Arg Val Ile Val Leu Pro Asp Gly 405 cag tac atg gaa atc gtg gaa ttg gcg Gin Tyr Met Giu Ilie Vai Giu Leu Ala 420 425 gct <210> 286 <211> 425 <212> PRT <213> Corynebacterium giutamicun <400> 286 Ile Arg Lys Tyr Ser Arg Leu Giu Giu 1 5 Tyr Giu Ala Asp Ala Giu Ala Ala Gin 25 Giu Ala Arq Ile Leu Asp Gin Gin Leu 40 Arg Arg Arg Val Giu Leu Ala Gin Ile Ser Gly Lys Ser Lys Thr Thr Leu Leu Leu Asp Ala Asp Ser Ile Thr Trp Leu Giu Gly Gly Leu Ile Met Ile Ser His 100 105 Val Cys Asn Lys Ile Trp Tyr Leu Asp 115 120 PCTIBOO/00926 gac caa cca gca gga aca Asp Gin Pro Ala Gly Thr 330 335 ctg gcc acc ctc gtg tcc Leu Ala Thr Leu Val Ser 350 ccc acc aac aac ctt gac Pro Thr Asn Asn Leu Asp 365 ctg cgc acc tac acc ggc Leu Arg Thr Tyr Thr Gly 380 qca gtc aag gcc ctt gag Ala Val Lys Ala Leu Giu 395 acc gag gat ctt tgg aat Thr Giu Asp Leu Trp Asn 410 415 taggttctaa ggctgtttat ctc Leu tcc Ser Ccg Pro gca Ala cca Pro 400 gat Asp 1008 1056 1104 1152 1200 1248 1295 1298 Ser Asn Ser Ala Pro Leu Le u Se r 125 Leu Leu Gly Thr Thr Ala Leu 110 Glu Gly Gly Gly Asn Asn Lys Ci y Ala WO 01/00805 PCT/IB00/00926 Val Tyr Asn Met Gly Phe Ser Lys Tyr Val Asp Ala Arg Ala Leu Asp 130 135 140 Glu Ala Arg Arg Arg Arg Glu Arg Ala Asn Ala Glu Lys Lys Ala Gly 145 150 155 160 Ala Leu Lys Asp Gin Ala Ala Arg Leu Gly Ala Lys Ala Thr Lys Ala 165 170 175 Ala Ala Ala Lys Gin Met Ile Ala Arg Ala Glu Arg Met Ile Asp Asn 180 185 190 Leu Asp Glu Ile Arg Val Ala Asp Arg Ala Ala Asn Ile Val Phe Pro 195 200 205 Glu Pro Ala Pro Cys Gly Lys Thr Pro Leu Asn Ala Lys Gly Leu Thr 210 215 220 Lys Met Tyr Gly Ser Leu Glu Val Phe Ala Gly Val Asp Leu Ala Ile 225 230 235 240 Asp Lys Gly Ser Arg Val Val Val Leu Gly Phe Asn Gly Ala Gly Lys 245 250 255 Thr Thr Leu Leu Lys Leu Leu Ala Gly Val Glu Arg Thr Asp Gly Glu 260 265 270 Gly Gly Ile Val Thr Gly Tyr Gly Leu Lys Ile Gly Tyr Phe Ala Gin 275 280 285 Glu His Asp Thr Ile Asp Pro Asp Lys Ser Val Trp Gin Asn Thr Ile 290 295 300 Glu Ala Cys Ala Asp Ala Asp Gln Gin Ser Leu Arg Ser Leu Leu Gly 305 310 315 320 Ser Phe Met Phe Ser Gly Glu Gin Leu Asp Gin Pro Ala Gly Thr Leu 325 330 335 Ser Gly Gly Glu Lys Thr Arg Leu Ala Leu Ala Thr Leu Val Ser Ser 340 345 350 Arg Ala Asn Val Leu Leu Leu Asp Glu Pro Thr Asn Asn Leu Asp Pro 355 360 365 Ile Ser Arg Glu Gln Val Leu Asp Ala Leu Arg Thr Tyr' Thr Gly Ala 370 375 380 Val Val Leu Val Thr His Asp Pro Gly Ala Val Lys Ala Leu Glu Pro 385 390 395 400 Glu Arg Val Ile Val Leu Pro Asp Gly Thr Glu Asp Leu Trp Asn Asp 405 410 415 Gin Tyr Met Glu Ile Val Glu Leu Ala 420 425 <210> 287 <211> 1812 WO 01/00805 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1789) <223> RXN00164 <400> 287 ctgctttqcg ggaggttatg aaatgagtgg ggagacqtcg PCT/IBOO/00926 aaaagcatgc gctttccgtt ggccagcctg ccgcaaqtgc gqcgcgaggt ggcccggcag gtg ggt cgt att ccg Val Gly Arg Ile Pro Cgg Arg gct Ala ctg Le u att Ile ttc Ph e ga a Glu ga t Asp gag Glu ctg Le u caa Gin 150 t cc Ser gcq Al a gcq Al a tat Tyr gtg Val1 ctc Leu tat T yr ga t Asp g cg Ala cta Leu ttt Phe 135 ttt Ph e aag Lys gcg Ala aag Lys 9g Ala tcc Ser att Ile gtg Val atg Met ggc Gly tcc Ser 120 acc Thr g tg Val1 cac His a tg Met ctg Leu gtg Val1 gat Asp gcg Ala aag Lys ttg Le u cgc Arg gtc Val gcg Al a gcg Al a ga t Asp 175 gta Val1 gtg Val ct g Leu ttt Phe gtg ValI att Ile ccc Pro tcc Se r ccg Pro ctg Leu ccg Pro 160 c9g Arg ct t Leu 1 ttg Leu ggg Giy gt t Val1 ctc Le u atc Ile acg Thr acc Thr att Ile ttt Phe 145 gtg Val1 tat Tyr gag Glu ctg Le u c9g Arg gag Glu agt Se r gcc Al a cac His gat Asp tta Leu 130 tct Ser tac Tyr gcg Ala gct Ala gcg Ala gtg Val1 agt Ser t gc Cys ttg Leu gtg Val 100 gtc Val1 tcc Ser ga c Asp t tc Phe gaa Giu 180 cgc Arg WO 01/00805 WO 0100805PCT/1BOO/00926 cg t Arg att Ile acc Th r 230 a tg Met gcg Ala ctg Leu t cc Ser ccg Pro 310 ga a Giu ga c Asp gca Ala cgg Arg cac His 390 gag Giu aaa Lys gca Ala gat Asp 215 acc Thr ctc Leu ctg Leu cg t Arg ggc Gly 295 att Ile ttg Leu atc Ile tct Ser gtg Val1 375 ctc Leu gtt Val1 cca Pro act Thr 200 ca g Gin a tg Met g cg Ala a cg Thr ggc Giy 280 tat Tyr cct Pro cg c Arg ga c Asp ggc Gly 360 cca Pro tct Ser cat His qat Asp gtg Val1 gcg Aila ttg Leu gtc Val1 atc Ile 265 ccg Pro gcg Aila gtg Val1 aac Asn atc Ile 345 gca Aia ga t Asp gac Asp gt t Va I gcc Ala 425 c gg Arg t cg Ser att Ile g cg Aia 250 ggc Gly atg Met tcg Ser ccc Pro gtc Val1 330 a cg Thr ggt Giy caa Gin cgc Arg ttc Phe 410 tcc Ser gcg Ala tgg Trp ttg Leu 235 ttg Leu gcq Ala aat Asn ctg Le u gac Asp 315 agc Ser atc Ile aag Lys ggg Gly gag Glu 395 tcc Ser gat Asp tat Tyr tct Ser 220 aac Asn gtg Val1 gtt Val1 atg Met g cg Ala 300 agc Ser ttt Phe aat Asn a cg Th r caa Gin 380 cg t Arg 99c Gly gag Glu tcc Ser 205 gtg ValI atg Met atc Ile acc Thr ttc Ph e 285 cg c Arg gg t Gly agc Ser tcc Se r a cg Thr 365 gt g Val1 atc Ile acg Th r gaa Giu a tg Met gtg Val1 t gg Trp ggc Gly gg t Gi y 270 at g Met atc Ile gtg Val1 tat T yr ggc Gi y 350 gtc Val1 ctt Leu gcc Ala ctg Leu tta Le u 430 ga t Asp aag Lys c tg Leu 240 aag Lys gtg Val gtg Val1 gga Giy gca Al a 320 ga t Asp act Th r gcc Al a gac Asp ttg Le u 400 cag Gin gcc Ala ggt Gly 225 ttt Phe ctg Leu ctg Le u ct c Leu gtt Val1 305 cct Pro t cc Ser gtc Val ttg Leu gac Asp 385 gcc Ala ga t Asp atg cat aat cag Met 210 att Ile g cg Ala gt c Val1 atg Met ga c Asp 290 gt t Vai ca g Gin tgg Trp gcq Ala ct g Leu 370 ttc Phe atg Met ctc Le u His cg t Arg gaa Giu att Ile att.

Ile 275 acc Thr gcg Ala ggc Giy gcg Ala ctc L eu 355 gcg Al a ccc Pro gt c Val1 acc Thr Asn g Cg Ala ttc Phe gat Asp 260 att Ile att Ile ga t Asp aaa Lys gtg Val1 340 gt g Val1 ggc Gi y gtc Val1 agc Ser ttg Leu 420 Gin cgc Arg ctc Leu 245 aat As n cgt Arg caa Gin ccg Pro gtg Val1 325 aaa Lys ggc Gly t tg Leu tct Ser ca g Gin 405 gct Ala 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 14.11 cat gct ctt His Ala Leu ggg caa gtt Gly Gin Val 435 aat Asn gtt Val1 gcg Al a 470 ga a Gi u gct Ala cgg Arg ggg Gly qgt Gly 550 WO 01/00805 gcc ctt g~ Ala Leu A~ 440 ggt gcg Cc Gly Ala Ai 455 ttg gcc cc Leu Ala Ar gcc acg gc Ala Thr Al gca gat gc Ala Asp Al ttq gat ca Leu Asp Gl 520 gag gtt gt Glu Val Va 535 att tat ca Ile Tyr Gl Ic :a 7g .1 n tgg ttg gag Trp Leu Glu gga atc cag Gly Ile Gin 460 gtg ttg ttg Val Leu Leu 475 gaa gca gga Glu Ala Gly 490 gtg agc aag Val Ser Lys gca tcg cgg Ala Ser Arg gaa tcc qgt Glu Ser Gly 540 cqt ctg tgq Arg Leu Trp 555 ag t Ser 445 cta Leu ctc Leu t cg Ser aac As n gct Ala 525 act Thr act Th r ctt Le u gaa Glu aa t Asn gcg Al a cgt Arg 510 ga t Asp cac His 9c9 Ala qga Gly gtg Val1 465 atc Ile agc Se r ttg Le u ctg Leu tta Le u 545 qtC Val ctg Leu 450 gc t Ala gtc Val g ca Ala gtg Val g tg Val1 530 ttg Le u ga Gly gac Asp cag Gin atc I le ctg Leu gtg Val1 515 a tg Met gat Asp aga Arq a cg Thr cag Gin atg Met gaa Glu 500 g cg Ala gat Asp cac His PCT/EBOO/00926 gtc 1459 Vali ttg 1507 Leu gat 1555 Asp 485 gag 1603 Gl u ca~c 1651 His aag 1699 Lys ggg 1747 Gl y 1789 1812 taqttqactq ttcaatgcgt tga <210> 288 <211> 563 <212> PRT <213> Corynebacterium glutamicum <400> 288 Val Giy Arg Ile Pro Arg Ala Lys Trp 1 5 Leu Leu Ser Ala Gly Aia Tyr Ala Ser Gly Arg Ile Val Asp Leu Val Ser Asp Val Giu Leu Ser Val Ile Leu Ile Ala 55 Leu Ser Ala Cys Gly Phe Tyr Val Val Ile Ala Asn Leu Arg Giu Asp Met Val Thr His Gln Val Glu Asp Ala Gly Ser Trp Val1 Gi y ValI Ser Gl y Gly Leu Val G ln Ile Ile Ala Leu WO 01/00805 WO 0100805PCTIBOOI00926 Thr Ile Ph e 145 Val Tyr Glu Ala Gly 225 Phe Leu Leu Le u Val 305 Pro Se r Val Leu Asp 385 Ala Val Ser Asp Phe Glu 180 Arg Asn Al a Phe Asp 260 Ile Ile Asp Lys Val1 340 Val1 Gl y Val1 Se r Ser Glu Leu Ser Ala Ala Vai Thr Glu Thr Val Pro Ser Trp Ala 165 Arg Gly Gin Arg Leu 245 Asn Arq Gin Pro Val1 325 Lys Gly Leu Se r Gin 405 Leo Gin 150 Ser Al a Arg Ile Th r 230 Met Al a Leu Ser Pro 310 Giu Asp Ala Arg His 390 Glu Ph e 135 Phe Lys Ala Al a Asp 215 Thr Leo Leu Arq Gly 295 Ilie Leu Ile Se r Val1 375 Le u Val1 Ile Leo T yr Ala 185 Val1 Ala Leo Val Ile 265 Pro Ala Val1 As n Ile 345 Ala Asp Asp Val 125 Aia Ile Leo 170 Glu A rc Ser Ilie Ala 250 Gly Met Ser Pro Val1 330 Thr Gly Gin Arq Phe 410 Ser Ala Pro 155 Ser Arg Ala T rp, Leo 235 Leo Ala As n Leo Asp 315 Ser Ile Lys Gly Giu 395 Se r Asp Thr 140 Val1 Ly s Al a Tyr Ser 220 As n Val1 Val1 Met Al a 300 Ser Phe Asn Thr Gin 380 Arg Gly Glu Ile Val1 Ala Arg Ser 205 Vai Met Ilie Th r Phe 285 Arq, Gi y Ser Ser Thr 365 Vali Ile Th r Glu Ile Val1 Pro Lys 190 Met Val1 T rp Gly Gly 270 Met Ile ValI Tyr Gly 350 Val1 Leo Ala Leo Leo 430 Ala Ala Asp 175 Val1 Glu Val1 Met Tyr 255 Ala Arq Val1 Lys Gly 335 Glu Aia Val Arg Arg 415 Ala Leo Pro 160 Arg Leo Asp Lys Leu 240 Lys Val1 Val1 Gly Ala 320 Asp Th r Ala Asp Leo 400 Gin His Asp Leo Thr Leo Ala Lys Pro Asp Ala WO 01/00805 Ala Leu Gly Gin 435 Gly Leu Asp Thr 450 Val Ala Gin Gin 465 Ile Val Ile Met Ser Ala Leu Glu 500 Leu Val Val Ala 515 Leu Val Met Asp 530 Leu Leu Asp His 545 Val Gly Arg PCT/IBOO/00926 Ala Gi y 455 Leu Ala Ala Leu Glu 535 Ile Le u Ile Leu 475 Al a Ser Ser Ser Leu 555 Leu Giu As n Ala Arg 510 Asp His Al a Pro Pro Pro Gi y 495 Se r Gin Gin Trp Glu Val Ala 480 Ala Ala Ile Glu Ser 560 <210> 289 <211> 1812 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1789) <223> FRXA00164 <400> 289 ctgctttgcg ggaggttatg aaatgagtgg ggagacgtcg ggccagcctg ccgcaagtgc qgcgcqaggt ggcccggcag Cgg Arg gc t Ala ctg Leu att Ile ttt Phe ctg Le u gcg Ala gca Al a aaaagcatgc gctttccgtt gtg ggt cgt att ccg Val Gly Arg Ile Pro 1 ttg ctg agt gcq ggc Leu Leu Ser Ala Gly ggg cqg att gtg gat Gly Arg Ile Val Asp gtt gag ctc agt gtg Val Glu Leu Ser Val ctc agt gcg tgc ggg Leu Ser Ala Cys Gly ttc Phe ga a Glu gat Asp gag Giu ctg Leu caa Gin 150 tcc Ser gcg Ala cg t Arg att Ile a cc Thr 230 atg Met gcg Ala ctg Le u WO 01/00805 tat gtg gtg tcg cgg Tyr Val Val Ser Arg 75 gat atg gtq ggc acc Asp Met Val Gly Thr gcg ggc tct ggc gat Ala Gly Ser Gly Asp 105 cta tcc gca gcg gtg Leu Ser Ala Ala Val 120 ttt acc att gcc gcg Phe Thr Ile Ala Ala 135 ttt gtg ctc att cct Phe Val Leu Ile Pro 155 aag cac tat ttg agc Lys His Tyr Leu Ser 170 gcg atg gcg gag cgt Ala Met Ala Glu Arg 185 gca act gtg cgg gcg Ala Thr Val Arg Ala 200 gat cag qcq tcg tgg Asp Gin Ala Ser Trp 215 acc atg ttg att ttg Thr Met Leu Ile Leu 235 ctc gcg gtc gcg ttg Leu Ala Val Ala Leu 250 ctg acg atc ggc gcg Leu Thr Ile Gly Ala 265 cgt ggc ccg atg aat Arg Gly Pro Met Asn 280 .PCTIBOO/00926 ttg agg 355 Leu Arg att tct gag Ile Ser Giu aag att atc Lys Ile Ile gcc aat Ala Asn g cg Ala t tg Leu aca Thr a cg Th r 140 gtc Val aag Lys gcg Ala tat T yr tct Se r 220 aac Asn gt g Val1 gtt Val1 atg Met ctt Leu gtg Val1 gag Glu 125 atc Ile gtg Vai q cg Ala cga Arg tcc Se r 205 gtg Val1 atg Met atc Ile acc Thr ttc Phe 285 ggg Gly agc Ser 110 acc Th r att Ile gtg Val ccg Pro aag Lys 190 atq Met gtg Val1 t gg Trp ggc Giy ggt Gly 270 atg Met t tg Le u 95 cg c Arg gt c Val1 qc9 Ala g cg Ala ga t Asp 175 gta ValI gaa Glu gt c Val1 atg Met tac Tyr 255 g cc Ala cg c Arg cac His qa t Asp tta Le u 130 tct Ser tac Tyr qcg Ala gct Al a atg Met 210 att Ile g cg Ala gt c Val1 a tg Met gac Asp 290 gtg Val 100 gt c Val1 t CC Se r ga c Asp ttc Phe gaa Glu 180 cgc Arg aat Asn g cg Ala ttc Phe gat Asp 260 att Ile att Ile gaa Glu tc Ser tca Se r tgg Trp gcg Ala 165 cg c Arg qgg Gly ca g Gin cgc Arg ct C Leu 245 aat Asn cg t Ar g caa Gin tcc ggc Ser Gly 295 tat gcg tcg Tyr Ala Ser ctg gcg Leu Ala 300 cgc atc gtg gga Arg Ile Val Gly gtt gcg gat ccg Val Ala Asp Pro 1027 WO 01/00805 WO 0100805PCT[IB00100926 cog att cot Pro 310 ga a Glu gao Asp goa Al a og g Arg cac His 390 gag Glu aaa aat As n gtt ValI gog Al a 470 gaa Glu got Ala cgg Arg ggg Gly g9t Ile ttq Leu atc Ile tot Ser gtg Val 375 ctc Leu gtt Val1 oca Pro g cc Ala ggt GI y 455 ttg Le u goo Ala gca Al a ttg Le u gag Glu 535 att Pro og c Arg gaoc Asp ggc Gly 360 cca Pro tct Se r cat His ga t Asp Ott Leu 440 gcg Ala gcc Ala a cg Thr ga t Asp gat Asp 520 gtt Val1 tat coo gao ago Pro Asp Ser 315 gtc ago ttt Val Ser Phe 330 acg atc aat Thr Ile Asn ggt aag acg Gly Lys Thr oaa ggg caa Gin Gly Gin 380 cgc gag ogt Arg Glu Arg 395 tto toc ggc Phe Ser Gly 410 too gat gag Ser Asp Glu tgg ttg gag Trp Leu Glu gga atc cag Gly Ile Gin 460 gtg ttg ttg Val Leu Leu 475 gaa goa gga Giu Ala Gly 490 gtg ago aag Val Ser Lys goa tog ogg Ala Ser Arg gaa too ggt Glu Ser Gly 540 ogt otg tgg gg t Gly ago Ser too Ser a og Thr 365 gtg Val1 ato Ile a og Thr gaa Glu agt Ser 445 ota Le u oto Leu tog Ser a ao Asn got Ala 525 act Thr act gg 0 Gly gog Ala oto Leu 355 gog Ala 000 Pro gt o Val1 aoo Th r ggg Gi y 435 gao Asp oa g Gln ato Ile otg Le u gtg Val1 515 atg Met gat Asp a ga aaa Lys gtg Val 340 gtg Val1 Gly gto Val1 ago Ser ttg Le u 420 oaa Gin acg Thr oag Gin atg Met gaa Glu 500 gog Al a gat Asp oao His gt g Val1 325 aaa Lys gg o Gly ttg Le u tot Ser cag Gin 405 got Ala gtt Val gt o Val1 ttg Leu gat Asp 485 gag Glu oao His aag Lys g gg Gi y 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1789 WO 01/00805 PCT11B00100926 Gly Ile Tyr Gin Arg Leu Trp Thr Ala Trp Ser Val Gly Arg 550 555 560 tagttgactg ttcaatgcgt tga 1812 <210> 290 <211> 563 <212> PRT <213> Corynebacterium glutamicum <400> 290 Val Gly Arg Ile Pro Arg Ala Lys Trp Trp Phe Leu Gly Ala Leu Val 1 5 10 Leu Leu Ser Ala Gly Ala Tyr Ala Ser Val Leu Val Pro Gin Val Leu 25 Gly Arg Ile Val Asp Leu Val Ser Asp Gly Ala Gin Met Arg Asp Phe 40 Val Glu Leu Ser Val Ile Leu Ile Ala Val Ala Ile Ala Gly Ala Val 55 Leu Ser Ala Cys Gly Phe Tyr Val Val Ser Arg Ile Ser Glu Lys Ile 70 75 Ile Ala Asn Leu Arg Giu Asp Met Val Gly Thr Ala Leu Gly Leu Pro 90 Thr His Gin Vai Giu Asp Ala Gly Ser Gly Asp Leu Val Ser Arg Ser 100 105 110 Thr Asp Asp Vai Ser Glu Leu Ser Ala Ala Vai Thr Glu Thr Val Pro 115 120 125 Ile Leu Ser Ser Ser Leu Phe Thr Ile Ala Ala Thr Ile Ilie Ala Leu 130 135 140 Phe Ser Leu Asp Trp Gin Phe Val Leu Ile Pro Val Val Vai Ala Pro 145 150 155 160 Val Tyr Tyr Phe Ala Ser Lys His Tyr Leu Ser Lys Ala Pro Asp Arg 165 170 175 Tyr Ala Ala Giu Arg Ala Ala Met Ala Glu Arg Ala Arg Lys Val Leu 180 185 190 Glu Ala Ile Arg Gly Arg Ala Thr Val Arg Ala Tyr Ser Met Giu Asp 195 200 205 Ala Met His Asn Gin Ile Asp Gin Ala Ser Trp Ser Vai Val Val Lys 210 215 220 Giy Ile Arg Ala Arg Thr Thr Met Leu Ile Leu Asn Met Trp Met Leu 225 230 -235 240 Phe Ala Glu Phe Leu Met Leu Ala Val Ala Leu Val Ile Gly Tyr Lys 245 250 255 Leu Val Ile Asp Asn Ala Leu Thr Ilie Gly Ala Val Thr Gly Ala Val WO 01/00805 WO 0100805PCT/IBOOIO0926 Leu Le u Val1 305 Pro Se r Val1 Le u Asp 385 Ala Asp Ala Gly Val 465 Ile Ser Le u Leu Leu 545 Val1 Met Asp 290 Val1 Gin Trp Ala Leu 370 Ph e Met Leu Le u Le u 450 Al a Va 1 Al a Val1 Val 530 Leu Gly 260 Ile Ile 275 Thr Ile Ala Asp Gly Lys Ala Val 340 Leu Val 355 Ala Gly Pro Val Val Ser Thr Leu 420 Gly Gin 435 Asp Thr Gin Gin Ile Met Leu Giu 500 Val Ala 515 Met Asp Asp His Arg Arg Leu Arg G1ly Pro Met Asn Met Phe Met Arg Val Gin Pro Val1 325 Lys Gly Leu Se r Gin 405 Ala Val1 Val Leu Asp 485 Glu His Lys Gly Ser Pro 310 Giu Asp Ala Arg His 390 Giu Lys Asn Val Ala 470 Giu Ala Arg Gly Gly 550 Gi y 295 Ile Le u Ile Ser Val1 375 Leu Val1 Pro Al a Gly 455 Leu Ala Ala Le u Glu 535 Ile 280 Tyr Pro Arg Asp Gly 360 Pro Se r His Asp Le u 440 Ala Ala Thr Asp Asp 520 Val Tyr Ala Val1 As n Ile 345 Aila Asp Asp Val1 Ala 425 Asp Arg Arg Ala Al a 505 Gin Val1 Gin Se r Pro Val1 330 Thr Gly Gin Arg Phe 410 S er Trp Giy Val1 Giu 490 ValI Aia Glu Arg Leu Asp 315 Se r Ile Lys Gly Giu 395 Se r Asp Leu Ile Leu 475 Al a Se r Se r Se r Leu 555 Aia 300 Se r Phe As n Thr Gin 380 Arg Gly Glu Giu Gin 460 Le u Gly Lys Arq Gi y 540 Trp 285 Arg Gly Se r Se r Thr 365 Vali Ile Thr Glu Ser 445 Leu Leu Se r Asn Ala 525 Th r Thr Ile Val1 Tyr Gly 350 Val1 Leu Aia Leu Leu 430 Leu Giu Asn Ala Arg 510 Asp His Ala Val Gly Lys Ala 320 Gly Asp 335 Giu Thr Ala Ala Val Asp Arg Leu 400 Arg Gin 415 Ala His Pro Giu Pro Val Pro Ala 480 Gly Ala 495 Ser Ala Gln Ile Gin Giu Trp Ser 560 <210> 291 WO 01/00805 <211> 1140 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1117) <223> RXN00243 <400> 291 cactgegcca gatttttgat gecgaeactg tggcaggtgt PCT[IBOO/00926 gcgcgctgag tacgaaaaat ttaaeaaagc agceatgat ggaaatgaag aggaaeagaa gtg acc agc gaa caa Val Thr Ser Glu Gin get Ala ttg Le u agg Arg ct g Leu ttt Phe ttg Le u ca g Gin etg Le u gtg Val tet Se r 150 etg Leu ttC tta Le u ate Ile gaa Glu att Ile a ac Asn gtt Val1 ga t Asp gtt ValI get Al a 135 t ca Se r gat Asp a ag ga t Asp aa c Asn gg a Gly gat Asp t ct Se r ac Th r ca g Gin gga Gly 120 att Ilie tat T yr t te Phe gaa ect Pro tgg Trp ate Ile geg Ala gee Al a tat Tyr att Ile 105 t tg Le u tte Phe tat Tyr etg Le u gee ate Ile gga Gi y tta Leu ate Ile gea Al a ate Ile gteC Val aet Th r tat Tyr gg t Gly aaa Ly s 170 ate eea Pro tte Phe ace Th r geg Ala 60 get Ala gge Gly a eg Th r tee Ser aaa Lys 140 ttt Phe gge Gly age gg t Gi y cac His gg t Gly 45 gt a ValI aat Asn get Ala tac Tyr aac Asn 125 t eg Ser ec Pro ate Ile gag cag Gin g ga Gly 30 ggt Gly ttg Leu act Thr t g Trp eta Leu 110 gg t Gly gga Gly gt t Val1 ga t Asp ea g ttc Phe 15 aeg Thr t eg Se r ett Le u ceg Pro eg t Arg 95 eg t Arg ga a Giu eac His gat Asp aaa Lys 175 cat cgt Arg gtg ValI gga Gly ceg Pro egg Arg 80 geg Ala ec Pro ggC Gly aat Asn eaa Gin 160 eg e Arg tet 1 ctt Leu ga c Asp tea Ser eaa Gin aat Asn ca g Gin ege Arg gte Val1 tta Leu 145 ga e Asp ca g Gin gt a tet Ser att Ile gga Gi y gga Gly aag Lys gag Giu gca Ala gag Glu 130 ace Thr ate Ile ate Ile tte egg Arg c et Pro aaa Lys aag Ly s gga Gly ga t Asp ace Thr 115 eac His tee Ser aat Asn a ga Arg tee att Ile gtg Val1 tee Se r ct g Leu eg e Arg ceg Pro 100 tat Tyr ace Thr gat Asp geg Al a get Ala 180 ggC ea g Gin ace Thr a eg Th r a gg Arg agt Se r Ct g Le u t eg Se r ttg Le u att Ile etg Le u 165 get Ala a gg 115 163 211 259 307 355 403 451 499 547 595 643 691 Phe ttt Ph e cac His cg g Arg 230 9CC Ala ga t Asp aat Asn aag Lys gaa Glu 310 a gg WO 01/00805 Lys Giu A: 1i aga agc cC Arg Ser Ai 200 cgc gcg cz Arg Ala GI 215 gat tac at Asp Tyr Me gtg gaa ca Val Giu Gl att aaa cg Ile Lys Ar 26 cgg cga ga Arg Arg GI 280 aag gcg ct Lys Ala Le 295 ccg ctg qt Pro Leu Va tgg agt cc la 35 '9 -n '9 '9 '9 t 1 Ile t tg Leu t C9 Se r ctg Le u ttC Phe 250 cag Gin aa a Lys ccg Pro cga Arg cca Ph e 999 Gly 9C9 Ala gt 9 Val 235 caa Gin atc Ile 9C9 Ala act Thr caa Gin 315 aa a Se r atc Ile aaa Lys 220 gaa Gi u qac Asp ca c His caa Gin gtc Val 300 ttt Phe ttg Giu tcc Ser 205 ga t Asp ccg Pro ctt Le u acc Thr ca g Gin 285 Ci99 Gly act Thr a gja Arg Gin 190 agt Ser ctt Leu (gat Asp gaa Glu ct 9 Leu 270 t cc Ser aat Asn gtC Val1 ca 9 Gin His gag Glu caa Gin a c9 Thr 99 t Giy 255 ga t Asp aaa Lys cgc Arg gag Giu atc Ile Ser qa a Giu agc Ser ttc Phe 240 gCt Ala cct Pro ga t Asp att Ile caa Gin 320 gtg Val1 Val1 9Ct Ala ttg Le u 225 agc Se r tat Tyr t tq Leu cat His a ag Lys 305 a cg Th r ccc Pro Ph e t tg Leu 210 ga t Asp att Ile gag Giu gtg Val1 gcc Ala 290 aag Lys ca g Gin gcg Al a Ser 195 ctg Le u gat Asp gcc Ala ca g Gin ca g Gin 275 aat Asn ga a Giu cg a Arg aa a Ly s Gly ttg Le u cta Le u aaa Ly s gt c Val1 260 ctg Le u 9 ca Ala gag Giu agt Ser PCT/IBOO/00926 Arg ttg 739 Le u ttt 787 Phe act 835 Thr 245 gaa 883 Glu aag 931 Lys ctg 979 Le u caa 1027 Gin cga 1075 Arg 325 Arg Trp Ser Pro Pro Lys Leu 1117 1140 tgaaaaccct cgcgcacgac aac <210> 292 <211> 339 <212> PRT <213> Corynebacterium giutamicum <400> 292 Val Thr Ser Glu Gin Ala Leu Asp Pro Ile 1 5 10 Leu Ser Arg Ile Gin Leu Ile Asn Trp Gly 25 Asp Ile Pro Val Thr Arg Giu Gly Ile Leu 40 Ser Giy Lys Ser Thr Leu Ile Asp Ala Ile 55 Gin Gly Lys Leu Arq Phe Asn Ser Ala Ala His Pro Giy Gin Phe Arg Thr Phe His Giy Thr Val Val Thr Gly Giy Ser Gly Thr Ala Val Leu Leu Pro Gin Ala Asn Thr Pro Arg WO 01/00805 PCT/IBOO/00926 70 75 Asn Lys Gly Arg Ser Leu Val Thr Tyr Ile Arg Gly Ala Trp Arg Ala 90 Gin Giu Asp Pro Leu Gin Asp Gin Ile Val Ser Thr Tyr Leu Arg Pro 100 105 110 Arg Ala Thr Tyr Ser Leu Val Gly Leu Thr Tyr Ser Asn Giy Giu Gly 115 120 125 Vai Giu His Thr Leu Val Aia Ile Phe Tyr Leu Lys Ser Giy His Asn 130 135 140 Leu Thr Ser Asp Ile Ser Ser Tyr Tyr Giy Vai Phe Pro Vai Asp Gin 145 150 155 160 Asp Ilie Asn Aia Leu Leu Asp Phe Leu Lys Glu Giy Ile Asp Lys Arg 165 170 175 Gin Ile Arg Ala Ala Phe Lys Giu Ala Ile Phe Ser Giu Gin His Ser 180 185 190 Vai Phe Ser Gly Arg Phe Arg Ser Arg Leu Gly Ile Ser Ser Giu Giu 195 200 205 Ala Leu Leu Leu Leu His Arg Ala Gin Ser Ala Lys Asp Leu Gin Ser 210 215 220 Leu Asp Asp Leu Phe Arg Asp Tyr Met Leu Val Giu Pro Asp Thr Phe 225 230 235 240 Ser Ile Ala Lys Thr Ala Val Glu Gin Phe Gin Asp Leu Giu Giy Ala 245 250 255 Tyr Giu Gin Val Giu Asp Ile Lys Arg Gin Ile His Thr Leu Asp Pro 260 265 270 Leu Val Gin Leu Lys Asn Arg Arg Giu Lys Ala Gin Gin Ser Lys Asp 275 280 285 His Ala Asn Ala Leu Lys Lys Ala Leu Pro Thr Val Gly Asn Arg Ile 290 295 300 Lys Lys Giu Giu Gin Giu Pro Leu Val Arg Gin Phe Thr Val Giu Gin 305 310 315 320 Thr Gin Arg Ser Arg Arg Trp Ser Pro Pro Lys Leu Arg Gin Ile Val 325 330 335 Pro Ala Lys <210> 293 <211> 1027 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS WO 01/00805 <222> (101)..(1027) <223> FRXA00243 (400> 293 cactgcgcca gatttttgat gccgacactg tggcaggtgt ttaacaaagc aqcccatgat ggaaatgaag aggaacagaa PCTJIBOO/00926 gcgcgctgag tacgaaaaat qct Ala ttg Le u agg Arg ct g Le u ttt Phe ttg Leu cag Gin ctg Leu gtg ValI tct Ser 150 ct g Le u ttc Phe ttt Ph e tta gat Leu Asp atc aac Ile Asn gaa gga Giu Giy att gat Ile Asp aac tct Asn Ser gtt acc Val Thr gat cag Asp Gin gtt gga Val Gly 120 gct att Ala Ile 135 tca tat Ser Tyr gat ttc Asp Phe aag gaa Lys Glu aga agc Arg Ser 200 cct Pro tgg Trp atc Ile gcg Ala gcc Ala tat Tyr att Ile 105 ttg Leu ttc Phe tat Tyr ctg Leu gcc Aia 185 cg t Arg atc Ile gga Giy tta Leu atc Ile g ca Ala.

atc Ile gt c Val1 act Thr tat Tyr gg t Gi y aaa Lys 170 atc Ile ttg Leu cac His acc Thr gtt Vai a cg Thr c ag Gin 75 cg t Arg tcc Ser tat T yr Ctg Le u gtg Val1 155 gag Glu ttt Phe ggg Gi y c ca Pro ttc Ph e acc Thr gcg Al a 60 gc t Ala ggc Giy acg Thr t cc Se r a aa Lys 140 ttt Phe gg c Gly agc Se r atc Ile gg t Gi y cac His ggt Gly 45 gta Vali aat Asn gct Aila tac T yr aa c As n 125 t cg Se r

CCC

Pro atc Ile gag Giu t cc Ser 205 cag Gin gga Gly 30 ggt Gly ttg Leu act Thr t gg Trp ct a Le u 110 ggt Gi y g ga Gly gtt ValI gat Asp cag Gin 190 agt Ser ttc Phe 15 a cg Thr t cg Ser ctt Le u C cg Pro cg t Arg 95 cg t Arg ga a Glu ca c His ga t Asp aaa Ly s 175 cat His gag Giu cgt Arg gt g Val1 gga Giy ccg Pro cgg Arg 80 gcg Ala ccc Pro ggc Gly aat Asn caa Gin 160 cgc Arg tct Se r gaa Glu gtg Vali 1 ctt Leu gac Asp t ca Ser ca a Gin aat Asn ca g Gin cg C Arg gt C ValI tta Leu 145 gac Asp ca g Gin gta ValI gc t Al a acc agc gaa caa Thr Ser Giu Gin t ct Se r att Ile gga Gly gga Giy aag Lys gag Giu gca Aia gag Giu 130 a cc Th r atc Ile atc Ile ttc Phe ttg Leu 210 cgg Arg cct Pro aaa Lys aag Lys g ga Gly gat Asp acc Thr 115 cac His t cc Ser aat Asn a ga Ar g tcc Ser 195 ct g Le u att Ile gtg Vali tcc Se r ctg Le u cg c Arg ccg Pro 100 tat Tyr acc Th r ga t Asp gcg Aia gct Ala 180 ggc Gly ttg *Leu ca g Gin acc Thr a cg Thr a gg Arg agt Ser ctg Le u t cg Ser t tg Le u att Ile ctg Le u 165 gct Ala agg Arg ttg Leu 115 163 211 259 307 355 403 451 499 547 595 643 691 739 wo 01/00805 WO 0100805PCTIBOOIOO926 cac cgc gcg cag tcg gcg aaa gat ctt caa agc ttg His Arg Ala Gin Ser Ala Lys Asp Leu 215 220 cgg gat tac atg ctg gtg gaa ccg gat Arq Asp Tyr Met Leu Val Giu Pro Asp 230 235 gcc gtg qaa caa ttc caa gac ctt qaa Ala Val Glu Gin Phe Gin Asp Len Giu 250 gat att aaa cgg cag atc cac acc ctg Asp Ile Lys Arg Gin Ile His Thr Leu 265 270 aat cgq cga gag aaa gcg caa cag tcc Asn Arg Arg Gin Lys Ala Gin Gin Ser 280 285 aag aag gcg ctg ccg act gtc ggg aat Lys Lys Ala Len Pro Thr Val Gly Asn 295 300 <210> 294 <211> 309 <212> PRT <213> Corynebacterium qintamicum <400> 294 Val Thr Ser Glu Gin Ala Leu Asp Pro 1 5 Len Ser Arg Ilie Gin Leu Ile Asn Trp 25 Asp Ile Pro Val Thr Arg Gin Gly Ile 40 Ser Gly Lys Ser Thr Leu Ile Asp Ala 55 Gin Gly Lys Len Arg Phe Asn Ser Ala Asn Lys Gly Arg Ser Len Val Thr Tyr Gin Giu Asp Pro Leu Gin Asp Gin Ile 100 105 Arg Ala Thr Tyr Ser Leu Val Gly Leu 115 120 Val Gin His Thr Leu Val Ala Ile Phe 130 135 Leu Thr Ser Asp Ile Ser Ser Tyr Tyr 145 150 Gin a cg Thr gt Gly 255 gat Asp aaa Lys cgc Arg Ile 10 Gly Len Ile Al a Ile 90 Val Thr Tyr Gly Len 225 agc Se r tat Tyr ttg Len cat His aa g Lys 305 Pro Phe Thr Ala Ala Gly Thr Ser Lys 140 Phe ga t Asp att Ile g ag Giu gtg Val1 g cc Ala 290 aag Lys Gi y His Gly Val1 Asn Al a Tyr As n 125 Se r Pro gat cta ttt Asp Len Phe gcc aaa act Ala Lys Thr 245 cag gtc gaa Gin Val Gin 260 cag ctg aag Gin Len Lys 275 aat gca ctg Asn Ala Leu gaa gag caa Giu Gin Gin 787 835 883 931 979 1027 Gin Gly Gly Leu Thr Trp Len 110 Gly Gi y Val1 Phe Thr Se r Len Pro Arg Arg Gin His Asp .WO1/00805 Asp Ile Asn Ala Leu Leu Asp Phe Leu Lys Glu 165 170 Gin Ile Arg Ala Ala Phe Lys Giu Ala Ile Phe 180 185 Vai Phe Ser Gly Arg Phe Arq Ser Arg Leu Gly 195 200 Ala Leu Leu Leu Leu His Arq Ala Gin Ser Ala 210 215 Leu Asp Asp Leu Phe Arg Asp Tyr Met Leu Vai 225 230 235 Ser Ile Ala Lys Thr Ala Val Giu Gin Phe Gin 245 250 Tyr Giu Gin Val Giu Asp Ile Lys Arg Gin Ile 260 265 Leu Val Gin Leu Lys Asn Arg Arg Giu Lys Ala 275 280 His Ala Asn Aia Leu Lys Lys Ala Leu Pro Thr 290 295 Lys Lys Glu Glu Gin 305 <210> 295 <211> 2325 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (2302) <223> RXA00259 <400> 295 ggccttatta acatacggcg gttctagcac acagcgatgg tgctgcatgt gccaagattt agcacggtac agtgctagaa Asp Gin 190 Se r Le u Asp Glu Le u 270 Se r Asn PCT1IBOO/00926 Arg Ser Giu Ser Phe 240 Al a Pro Asp Ile acc cca ttt Thr Pro Phe gat tca gta Asp Ser Val cgt ttc gag Arg Phe Glu tca gat gcg gca Ser Asp Ala Ala tct ggt cga gac Ser Gly Arg Asp cgt tct gcg tat Arg Ser Ala Tyr aac aac acg Asn Asn Thr 15 cct att act Pro Ile Thr 30 gcc cag ctg Ala Gin Leu atgatgtgtc ccaccgatat atg agc gga ctg ttt Met Ser Gly Leu Phe 1 gta aaa act gat gga Vai Lys Thr Asp Gly aag atc tct gag gat Lys Ile Ser Giu Asp gca aat ata atc tgc Ala Asn Ile Ile Cys WO 01/00805 PCTIBOO/00926 ggt cag 307 Gly Gin gat gtg gca Asp Val Ala tgg ggc agt Trp Gly Ser tct cta tcg Ser Leu Ser gcc tct gac Ala Ser Asp ggg acg gta Gly Thr Vai 120 cag aaa acc Gin Lys Thr 135 ttt ggt gtc Phe Gly Val 150 aac gct gta Asn Ala Val ctg tat aca Leu Tyr Thr ctc att gtc Leu Ile Val 200 ctg tta atg beu beu Met 215 tat ctt ttg Tyr beu Leu 230 tcg aca gct Ser Thr Ala aaa atc gtg Lys Ilie Val ata gag aaa Ile Giu Lys 280 tcg cta'tcg cct tgg Pro Trp ggt aag Gly Lys aac gaa Asn Glu ccg caa Pro Gin 105 cct gat Pro Asp ttt agc Phe Ser cta gaa Leu Giu tat aag Tyr Lys 170 cgt gct Arg Ala 185 gtc qat Val Asp aaa gta Lys Val gtt tat Val Tyr gta ggt Val Gly 250 cag tat Gin Tyr 265 gag ctc Giu Leu ggt gat gga gcg Gly Ala agc act gtt ttc agt ctt act Ser Thr Val Phe Ser Leu Thr aca Thr 75 aaa Lys tct Ser gat Asp a cg Thr g ca Ala 155 a ca Th r g CgC Ala ga t Asp ata Ilie ga a Glu 235 gg t Gly c ct Pro agt Se r cct tct Ser t gg Trp t tg Le u aag Ly s att Ile 140 ctt Leu t gg T rp aac Asn att Ile cgc Arg 220 gaa Giu agc Se r ttc Phe gca Al a ga a ctt Leu a ca Thr att Ile acc Thr 125 ggg Giy gcg Ala aaa Ly s cat His ga t Asp 205 ttg Le u gaa Glu ga a Glu ga t Asp tta be u 285 ga c gtt ValI atc Ile gag Giu 110 ggc Gly tca Se r ctc Le u cag Gin ttt Phe 190 cg c Arg ctt Leu tca Ser ga t Asp gt t Val1 270 ttt Phe ttt aat Asn gtt Val1 95 gag Glu caa Gin att Ile t ca Se r gag Giu 175 aaa Lys ctc Le u gga Gly ctg be u ga t Asp 255 c cg Pro gac Asp g ca ttg Le u 80 ga t Asp ttt Phe aag Lys g ca Ala aaa Lys 160 caa Gin ga t Asp cat His cg a Arg tta Le u 240 gc t Ala cc t Pro aag by s ct a att Ile ttc Phe tac Tyr atc Ile ggt Gly 145 gga Gly ga t Asp ctg Le u act Thr ttc Phe 225 a cg Th r ttg Leu ctg be u ctt beu gtg cgc Arg aac Asn cga Arg aaa bys 130 gg g Gi y gta Val1 t cg Ser aac Asn gat Asp 210 ccg Pro a cg Thr cg t Arg aca Th r ttc Phe 290 aag t cg Ser c cgC Pro gta Val1 115 act Thr gt c Val1 gat Asp tgg Trp aag Lys 195 ga a Glu ca g Gin cta Le u ttc Phe t ca Se r 275 ca g Gin t cg g aa Glu tgg Trp 100 atc Ile gtt Val1 gga Gly gct Ala cca Pro 180 cga Arg ttg beu g tg Val1 gcc Ala atg Met 260 ttt Phe gg t Gly aga ga a Glu gt g Val1 gt t Val1 ct g Le u ggg Gly gca Ala 165 a cg Th r att Ile gcg Ala aa t Asn aga Arg 245 gag Giu caa Gin gt t Val1 a tg 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 WO 01/00805 PC Ser Leu Ser Gly Asp Pro Giu Asp Phe Ala Leu Val Lys Ser Arg Met T111BOO/00926 295 ttc Phe 310 ttt Ph e gtt Val1 ccg Pro gga Gi y tgt Cys 390 ttg Leu cgt Arg ttt Phe cta Le u ga t Asp 470 att Ile tta Le u atg Met ggt Gly gat Asp gc t Ala aac Asn tct Se r ggt Gly 375 gag Gi u ttc Phe gag Giu caa Gin tct Se r 455 gac Asp ga t Asp ttc Phe agc Se r gca Ala gtc Val1 gc t Aila Giy gt g Val1 360 cga Arg ggg Gly ctt Leu agg Arg aga Arg 440 aat Asn aat As n cg a Arg acc Thr ggc Gly 520 t tg Le u tg Trp cta Le u gtt Val1 345 tat Tyr acg Thr atg Met ttc Phe gg g Gly 425 cc t Pro gcg Ala ga c Asp ct t Le u gct Ala 505 ata Ile cct Pro gaa Glu cta Leu 330 gat Asp aaa Lys aca Thr aac Asn cca Pro 410 atc Ile gga Gly gac Asp atg Met ga t Asp 490 qct Al a ttc Phe caa Gin aag Ly s 315 acc Thr ctc Le u cgt Arg ggc Gly aac Asn 395 cgt Arg t cq Se r cat His ga t Asp gtg Val 475 gga Giy aat As n a gt Ser tta Le u 300 act Thr aa c As n aca Th r ctt Le u t cg Ser 380 gag Giu ctt Le u acg Thr gtc Val1 gct Ala 460 g ca Ala gag Glu ggt Gly gac Asp c ca Pro Ctg Le u gga Gly cga Arg gt a Val1 370 ga a Giu aag Lys acg Thr acg Th r ttg Le u 450 tta Le u ctt Le u gca Ala cg t Arg tgc Cys 530 gag Glu 305 ttq Le u tca Ser att Ile 355 ttg Leu aag Lys acc Th r aga Arg tac Tyr 435 gat Asp att Ile cc t Pro gtt Val1 caa Gin 515 t cg Se r aaa Ly s 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 WO 01/00805 WO 0100805PCT[EBOO/00926 tct Ser 550 gag atg acg ctt Giu Met Thr Leu gag gct gct ttc Giu Ala Ala Phe tgg Trp 560 tta aac cag gtg Leu Asn Gin Val gaa agg gct aga, Giu Arg Ala Arg gcc Ala 570 tgt ggt aat gat Cys Gly Asn Asp agt ggc ctt gag Ser Gly Leu Giu ctt ttt Leu Phe 580 cgt aaa gtt Arg Lys Val aat cag gac Asn Gin Asp 600 ata aag acc gaa Ile Lys Thr Giu aga. att tta. agt Arg Ile Leu Ser gta ttg aag Val Leu Lys 595 ctt ggt att Leu Gly Ile ccc tca gat tgg Pro Ser Asp Trp tta ggt cca tat Leu Gly Pro Tyr t cg Ser 610 ttg gcg Leu Ala 615 aaa agc tcg aat Lys Ser Ser Asn tct tca gtg ctg Ser Ser Vai Leu tct ctg caa agt Ser Leu Gln Ser gg t Gly 630 ata gag gaa cat Ile Giu Giu His ca g Gin 635 ttt gat gtg ata Phe Asp Vai Ile att gga gtq ctt Ile Gly Vai Leu tta acg act gtg Leu Thr Thr Val tat Tyr 650 tct tcg cga cag Ser Ser Arg Gin cca agc ggt ggt Pro Ser Gly Giy gca tgg Ala Trp 660 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 2275 2322 2325 ata gat tct Ile Asp Ser cta cgg gct Leu Arg Ala 680 cag cat agt ctg Gin His Ser Leu tca cgg tac gta Ser Arg Tyr Vai cct gat tct Pro Asp Ser 675 ggt aag ata Giy Lys Ile ata acc aag tct Ile Thr Lys Ser gta gat gta gaa Val Asp Val Giu cta Le u 690 cag ttc Gin Phe 695 acg gat ttt agc Thr Asp Phe Ser gaa ggg aag cga Giu Giy Lys Arg gtt gtc gca tat Vai Vai Ala Tyr ctg gag act gga Leu Giu Thr Gly aga Arg 715 agt gat ttc act Ser Asp Phe Thr gaa. cga tta ggg Giu Arg Leu Giy tac agt atc gca.

Tyr Ser Ile Ala ga t Asp 730 tct ata gtc gat Ser Ile Vai Asp tgatgaggct gaggtcatga ctt <210> 296 <211> 734 <212> PRT <213> Corynebacterium giutamicum <400> 296 Met Ser Gly Leu Phe Thr Pro Phe Ser 1 5 Asp Ala Aia Lys Asn Asn*Thr 10 Val1 Lys Al a Se r Ile Phe Tyr Ile Gly 145 Gly Asp Leu Thr Phe 225 Thr Le u Leu Leu Val 305 Arg WO 01/00805 Lys Thr As Ile Ser G1 Asn Ile Il Leu Thr G1 Arg Ser G1 Asn Pro Tr Arg Val Ii 115 Lys Thr Va 130 Gly Val Gi Val Asp Al Ser Trp Pr 18 Asn Lys Ar 195 Asp Glu Le 210 Pro Gin Va Thr Leu Al.

Arg Phe Me 26 Thr Ser Ph 275 Phe Gin G1 290 Lys Ser Ar Leu Leu Hi i0 e y a 0 0 e 1 i a 0 0 e y 1 a Gi y Asp C ys Gin Glu Val Val Leu Giy Ala 165 Th r Ile Ala Asn Arg 245 Glu Gin Val1 Met Arg 325 Asp Ar g Asp Trp 70 Ser Ala Giy Gin Phe 150 Asn Leu Le u Leu Tyr 230 Se r Lys Ile Se r Phe 310 Phe Ser Phe Val1 55 Gly Le u Se r Thr Lys 135 Giy Al a T yr Ile Le u 215 Leu Thr Ile Giu Le u 295 Asp Ala Val Glu 40 Ala Ser Ser Asp Vali 120 Th r Val Val1 T hr Vali 200 Met Le u Ala Vai Lys 280 Ser Val1 Ala Se r Arg Pro Gi y Asn Pro 105 Pro Phe Le u Tyr Arg 185 Val1 Lys Val1 Val1 Gin 265 Glu GI y Trp Le u Gly Ser Trp Lys Giu 90 Gin Asp Ser Glu Lys 170 Ala Asp Val1 Tyr Gi y 250 T yr Le u Asp Glu Le u 330 Asp Tyr Al a Se r Trp Le u L ys Ile 140 Le u T rp As n Ile Arg 220 Giu Se r Phe Al a Glu 300 Thr Asn Leu Ser Ser Leu Thr Ile Thr 125 Gly Ala Lys His Asp 205 Leu Gi u Glu Asp Leu 285 Asp Le u T rp Ile Gl n Val1 Asn Val1 Glu Gin Ile Se r Giu 175 Lys Le u Gly Leu Asp 255 Pro Asp Al a Th r Arg 335 PCTIBOOOO926 Thr Le u Phe Leu Asp Phe Ala Lys 160 Gin Asp His Arg Leu 240 Ala Pro Lys Leu Pro 320 Ile WO 01/00805 WO 0100805PCT/IBOO/00926 Tyr Gly Ser Gly Giu Val Asn Giy Val. Asp Leu Thr Ile Leu Ala Thr Ilie Giu Trp 385 Le u Ser Asp Gin Asp 465 Lys Met Giy Al a Tyr 545 Le u Giy Le u T yr Lys 625 Ile Arq Val1 370 Glu Lys Thr Th r Le u 450 Le u Le u Al a Arg Cys 530 Glu Asn Le u Se r Ser 610 Ser Gly 345 350 Ser Gly 375 Glu Phe Gi u Gin Se r 455 Asp Asp Phe Ser Ala 535 Giu Arg Lys Gin Ala 615 Ilie Thr Val1 360 Ar g Gi y Le u Arg Arg 440 Asn Asn Arg Th r Gly 520 Le u Met Ala ValI Asp 600 Lys Glu Th r Tyr Thr Met Phe Gly 425 Pro Ala Asp Le u Al a 505 Ile Pro Thr Arq Asn 585 Pro Ser Giu Val1 Al a Pro Asp His 415 Tyr Asp Phe Met Arg 495 Asp Se r Gin Phe Val1 575 Arg Gly Val1 Ile Gly 655 Se r Lys Gly Leu 400 Pro Phe Glu Val1 Lys 480 His Lys His Leu Trp 560 Ser Ile Pro Leu Asp 640 Pro Arq 645 Ser Gly Gly Ala Trp Ilie Asp Ser Phe WO 01/00805 WO 0100805PCTIIBOOIOO926 660 665 670 Tyr Val Pro Asp Ser Leu Arg Ala Ile Thr Lys Ser Giu Val Asp Val 675 680 685 Glu Leu Gly Lys-Ile Gin Phe Thr Asp Phe Ser Trp Giu Gly Lys Arg 690 695 700 Lys Val Val Ala Tyr Ala Leu Giu Thr Gly Arq Ser Asp Phe Thr Arg 705 710 715 720 Giu Arg Leu Giy Gly Tyr Ser Ile Ala Asp Ser Ile Val Asp 725 730 <210> 297 <211> 789 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (766) <223> RXN00410 <400> 297 gtgttqatgc qttagtccac ccacgcagct acgccccaaa gqaataatct tgaaccctgc cacagataac gctccqccqg tcctttcagc ccaaqatctc atq atg atc tat qqa Met Met Ile Tyr Gly aaa Lys cag Gin a ca T hr aga Arg aat Asn ga t Asp ccg Pro atc qa a Glu tgg Trp cag Gin aac Asn aaq Lys gt q ValI qc q Al a ttg gtt Val1 acc Th r tgc Cys aaa Lys Ct 9 Leu cct Pro cat His aag cgg Arg t cc Se r ctt Leu aac Asn cg t Arg att Ile cgc Arg att ctc Leu atg Met gga Giy atc Ile a cg Thr t cg Se r gtg Val 110 aat 1 att Ile tca Ser cag Gin agc Ser agc Ser gac Asp cag Gin cg c tct Se r ggc Giy cca* Pro tcc Ser atg Met aat Asn tgg Trp cat gta Val1 t ct Ser acC Thr ctcj Le u qtg Val att Ile ttt P he 115 ttg cag Gin qqC Giy t ca Ser t ca Se r ttt Phe ttg Leu 100 ga a Giu CCt WO 01/00805 Ile Thr Ser Val Leu Lys Ilie Asp Asn 120 125 gag ctg tct ggc qgt caq caa caa cgc Giu Leu Ser Gly Giy Gin Gin Gin Arg 135 140 atg tct agg ccc gat att qtc att gcg Met Ser Arq Pro Asp Ile Val Ile Ala 150 155 gat tcc gtc acc agc gat gca gtq ttg Asp Ser Vai Thr Ser Asp Aia Val Leu 170 gat gat ttt ggg cag tca ctt gtg ttt Asp Asp Phe Giy Gin Ser Leu Vai Phe 185 190 gct cac cqt ggt gac gtg ttg atc aca Aia His Arq Gly Asp Val Leu Ile Thr 200 205 gat acg gca gat ttq cqq gtq ggq cgt Asp Thr Ala Asp Leu Arg Vai Giy Arg 215 220 gc t <210> 298 <211> 222 <212> PRT <213> Corynebacterium qiutamicum <400> 298 Met Met Ile Tyr Giy Lys Gly Ser Thr 1 5 Ile Ser Val Gin Ile Gin Ser Asp Lys Ser Giy Ser Gly Lys Thr Thr Leu Leu 40 Gin Pro Thr Ser Giy Arg Val Thr Leu Ser Ser Leu Ser Giu Asn Lys Arg Ala 70 Ser Met Val Phe Gin Asp Phe Asn Leu Asp Asn Ile Leu Leu Pro Leu Arg Leu 100 105 Gin Trp Phe Giu His Ilie Thr Ser Val 115 120 Arg His Leu Pro Gly Giu Leu Ser Giy Arg Met Arg His Leu Pro 130 gcc gcg att gcc cgg gcg Ala Ala Ile Ala Arg Ala 145 gat gag cca aca qga agt Asp Giu Pro Thr Giy Ser 160 aat ttg ttc cgc agc att Asn Leu Phe Arg Ser Ile 175 180 gtc acc cac gat aaa gat Val Thr His Asp Lys Asp 195 atg cgt gat ggc aaq atc Met Arg Asp Gly Lys Ile 210 taatgttcag gcttqctttc PCTJIBOO/00926 Giy ttg 547 Leu ttg 595 Leu 165 gtt 643 Val1 gct 691 Ala atc 739 Ile 786 Giu 10 Trp Gin Asn Lys Val1 90 Al a Le u Gly Ala Ile Ser As n Arg Leu Arg Asp 125 Gin Asp Giy Leu Th r His Val Asp Arg Al a Gly Gin Ala Le u Ile Lys Lys Met Ala WO 01/00805 130 135 Ilie Ala Arg Ala Leu Met Ser Arg Pro Asp Ilie 145 150 155 Pro Thr Giy Ser Leu Asp Ser Val Thr Ser Asp 165 170 Phe Arg Ser Ilie Val Asp Asp Phe Gly Gin Ser 180 185 His Asp Lys Asp Ala Ala His Arg Gly Asp Val 195 200 Asp Giy Lys Ile Ile Asp Thr Ala Asp Leu Arg 210 215 <210> 299 <211> 789 <212> DNA <213> Corynebacterium qiutamicum <220> <221> CDS <222> (101)..(766) <223> FRXA00410 <400> 299 gqtgatgc gttagtccac ccacgcaqct ac gccccaaa PCTIBOOIOO926 140 Val Ilie Ala Asp Ala Val Leu Asn 175 Leu Val Phe Val 190 Leu Ile Thr Met 205 Vai Giy Arg 220 ggaataatct tgaaccctgc cacaqataac gctccqccgg tcctttcagc ccaagatctc atq atg Met Met aaa Lys caq Gin a ca Thr aqa Arg aat As n ga t Asp ccg Pro gqa Gly tcc Se r act Thr gt g Val1 aag Lys ttc Ph e ttq Le u gaa Giu tgq Trp ca 9 Gin aac As n aaq Lys gtg Val1 qg Ala qct Ala atc Ile tcc Se r aac Asn cgc Arg ttg Leu ag Arg ctc Leu atg Met ga Gly atc Ile acq Thr t cg Se r qt g Val 1 att Ile tca Ser ca g Gin agc Se r agc Se r ga c Asp atc tat gqa Ile Tyr Gly gta cag att Val Gin Ile tct qgc aaa Ser Gly Lys acc tca qgc Thr Ser Gly ctg tca gaa Leu Ser Giu gtg ttt cag Val Phe Gin att ttg ctg Ile Leu Leu 100 115 163 211 259 307 355 403 45:1 cag tgg ttt Gin Trp Phe gaa cac Giu His WO 01/00805 WO 0100805PCT[EBOO/00926 105 atc acc agt gtg ttg aag att gat Ile Thr Ser Val Leu Lys Ile Asp 120 125 qag ctg tct ggc gqt cag caa caa Giu Leu Ser Gly Gly Gin Gin Gin 135 140 atg tct agg ccc gat att gtc att Met Ser Arg Pro Asp Ile Val Ile 150 155 gat tcc gtc acc agc gat gca gtg 1 Asp Ser Vai Thr Ser Asp Ala Val 1 170 gat gat ttt gqg cag tca ctt gq Asp Asp Phe Giy Gin Ser Leu ValI 185 gct cac cgt qgt gac gtg ttg atc Aia His Arq Gly Asp Vai Leu Ile 200 205 gat acg gca gat ttq cqg qtq gg Asp Thr Ala Asp Leu Arg Val Giy 1 215 220 gct <210> 300 <211> 222 <212> PRT <213> Corynebacterium giutarnicum (400> 300 Met Met Ile Tyr Giy Lys Giy Ser 1 Ile Ser Vai Gin Ile Gin Ser Asp 1 Ser Giy Ser Gly Lys Thr Thr Leu 40 Gin Pro Thr Ser Giy Arg Val Thr1 Ser Ser Leu Ser Giu Asn Lys ArgJ Ser Met Vai Phe Gin Asp Phe Asn Asp Asn Ile Leu Leu Pro Leu Arg I 100 Gin Trp Phe Giu His Ile Thr SerI 115 cgt atq cgc cat ttq cct Arg Met Arg His Leu Pro 130 gcc gcg att gcc cgq gcg Ala Aia Ile Ala Arq Ala 145 gat gag cca aca gga agt Asp Glu Pro Thr Giy Ser 160 aat ttg ttc cgc aqc att Asn Leu Phe Arg Ser Ile 175 180 gtc acc cac gat aaa gat Val Thr His Asp Lys Asp 195 atq cqt qat ggc aag atc Met Arg Asp Gly Lys Ile 210 taatgttcag gcttgctttc qgg Gly ttg Leu ttg Leu 165 gtt Val1 gc t Al a atc Ile Ala Ile Ser Asn Arg Le u Arg Asp Le u Met Gly Ile Thr Ser Val1 110 Asn WO 01/00805 115 120 Arg His Leu Pro Gly Giu Leu Ser Giy Giy Gin 130 135 Ile Ala Arq Ala Leu Met Ser Arg Pro Asp Ile 145 150 155 Pro Thr Gly Ser Leu Asp Ser Vai Thr Ser Asp 165 170 Phe Arg Ser Ilie Val Asp Asp Phe Gly Gin Ser 180 185 His Asp Lys Asp Ala Ala His Arg Gly Asp Val 195 200 Asp Giy Lys Ilie Ile Asp Thr Ala Asp Leu Arg 210 215 <210> 301 <211> 1500 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1477) <223> RXN00456 <400> 301 ctcaccaacc cggagatcgt cacaqcggtg ctaacgqatc tcqtcqcaga cttttqctca ttgccctagg tgtacttqgt PCTIIBOO/00926 125 Gin Arg Ile Ala Val *Leu Val, Phe 190 Ile Thr 205 Gly Arg ctq Leu cga Arq Ala gtq Val1 aat Asn acc Thr gcg Al a gca Al a ca g Gin cat His a cq Thr gcg Al a atg Me t gtt Val1 att Ilie gtg Val1 t cg Se r cg C Arg qtg Val1 gga Gly caa Gin cq t Arg ttg Leu aat Asn t cg Ser ttg Le u aaa Lys cg t Arg ggc Gi y 75 t gg Trp ttg Le u ctq Le u gta Val gaa Glu 60 gtg Val1 gtg Val agc Se r ctt Le u gtg Val1 ttg Leu act Thr gct Al a gcc Ala 15 acc Th r qaa Glu gg t Gly acc Thr qgC Gly 95 g cc Ala a cg Thr ga t Asp gct Al a cga Arg 80 att Ile atgcctagct tatgqcgtgc gtg ctg cag gca ctq Val Leu Gin Ala Leu 1 ata ctt gag gga aac Ile Leu Giu Gly Asn ttq gt ttq ggg gtq Leu Gly Leu Gly Val cta ggc cag cat tat Leu Gly Gin His Tyr gcg ctg gtq cct qga Ala Leu Val Pro Gly gcc agc aat gat ctc Ala Ser Asn Asp Leu gtt ccg atg gtc acc Val Pro Met Vai Thr 100 WO 01/00805 PTIO/02 PCTIIBOO/00926 ggg Gly ctc Leu gtg Val1 cg C Arg 150 gct Ala gca Ala t cc Se r ctt Leu gt t Val1 230 gaa Giu cgc Arg caa Gin tat T yr ggc Gly 310 gag Giu ctg Leu cqc Arg ctg Leu 135 aaa Lys gga Gly gtC Val1 tgg Trp ggc Gly 215 g cg Al a Ct t Le u atc Ile Ca a Gin gt C Val1 295 tct Se r ga a Glu ccq Pro a ca Thr 120 ccq Pro aaa Lys qaa Glu acc Thr g cc Ala 200 act Thr gga Gly ggC Giy ctq Le u aaa Lys 280 aaa Lys gca Ala 99c Gi y ttg Leu 105 99C Giy gtq Vali cg t Arg tta Le u cga Ar q 185 a cc Thr gtc Val1 ata Ile cgc Arq att Ile 265 Ct a Leu ggt Gly ga t Asp aca Thr att Ile gtg Val gtg Val1 qqa Giy ctg Le u 170 ga t Asp gqt Gly agc Ser atg Met gtg Val 250 cca Pro ccc Pro at t Ile gcg Al a gat Asp 330 g cg Ala gct Ala qcg Al a cg c Arg 155 cac His tcc Ser ttt Phe att Ile a tg Met 235 gtg Val1 ctt Leu ggg Giy tcc Ser a cq Thr 315 gta Vali gtg ValI act Thr 125 t gg Trp gct Al a aca Thr cga Arg cqc Arg 205 att Ile ctt Le u tat T yr caa Gin caa Gin 285 ctt Le u aaa Lys gtc Val gtg Val cc a Pro ttg Leu cgg Arg gca Ala 175 gtg Vali ttg Leu ggc Gi y qt t Val1 caa Gin 255 ggc Gi y aca Thr gga Gi y gt C Val1 gg t Gly 335 ttt Phe a tq Met 130 qca Ala gat Asp cqt Arg gct Ala atg Met 210 gaa Giu act Thr aaa Lys ttt P he atc Ile 290 atc: Ile ttg Le u ctt Le u atc Ile 115 tgt Cys cg t Arq tct Se r gag Glu gta Val1 195 gca Ala gta Val1 cca Pro g cc Al a aaa Lys 275 ccc Pro tac T yr tct Se r t cg Se r caa.

Gin gta Val1 ga a Giu gtc Val1 ct C Leu 180 aga Arg g cc Al a a gt Ser gt t Vali g cg Ala 260 cac His gg t Gly ctc Leu gca Al a ca g Gin 340 ga t Asp gcc Ala cta Leu atg Met 165 aat As n cg t Ar g tcg Se r gag Giu gca Al a 245 aca Thr tcC Se r gt C Val cac His a tg Met 325 ctt Le u 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 WO 01/00805 PTIO/02 PCT/IBOO/00926 cct ttg aaa caa cqa c~c gcc Ctc atc Pro Leu Lys Gin Arg Arg Ala Leu 345 cac tta agc cgt ggt tca qta tcg His Leu Ser Arg Gly Ser Vai Ser 360 365 gat qcc acc gtg gaa qaa att gaq Asp Ala Thr Val. Giu Giu Ile Giu 375 380 aac aac acc ggg aaa caa cgc ttg i Asn Asn Thr Gly Lys Gin Arg Leu 390 395 act tcg cag atc aac aaa ctg aaa i Thr Ser Gin Ile Asn Lys Leu LysI 41i0 cca ccg ctt ttg qta ctt gaa ggc z Pro Pro Leu Leu Val Leu Giu Giy 1 4254 tat ccc gga gtq atc atc tcc acc Tyr Pro Gly Vai Ile Ile Ser Thr 440 445 tgg cgg caa qtg aac atc taatctaqi Trp Arg Gin Vai Asn Ile 455 <210> 302 <2i1> 459 <212> PRT <213> Corynebacterium glutamicum <400> 302 Val. Leu Gin Ala Leu Leu Aia Ile 1 5 Ile Leu Giu Gly Asn Arg Ala Leu Leu Giy Leu Gly Val. Ala Gin Trp Leu Giy Gin His Tyr Val. His Giu Ala Leu Val Pro Gly Asn Thr Aia 70 Ala Ser Asn Asp Leu Thr Ala Vai Val Pro Met Val. Thr Giy Leu Pro 100 Leu Phe Ile Gin Asp Leu Arg Thr Ile 350 :gc krg ,a a ~aa ,ys ~tt Ele itc lie ~30 itt Ia 1 gga atc Gly Ile ctg qtt Leu Val gca ctg Ala Leu aac qgc Asn Gly 400 gcc aqc Ala Ser 415 acc cct Thr Pro caq gaq Gin Giu qcc tca gca Ala Ser Ala 355 ggt ttg cga Gly Leu Arq 370 gaa caa gtt Glu Gin Vai 385 gga cac ccc Gly His Pro qcc acc ctt Ala Thr Leu gaa aac ctc Giu Asn Leu 435 aac cca tcc Asn Pro Ser cac cac His His gqtg ccg Vai Pro qgt ctg Giy Leu tgg agt Trp Ser 405 cga acc Arq Thr 420 ctc aac Leu Asn gaa aca Giu Thr 1171 1219 1267 1315 1363 1411 1459 1500 ~a acatggcagg acg 2e t Ia 1 [le Ia 1 3er krq Leu 3 1Y Al a Thr Gi u Gi y Thr Giy Val1 Pro Ala Thr Asp Ala Arg Ile Ala Leu WO 01/00805 WO 0100805PCTIBOO/00926 Leu Arq 145 Ala Asp Al a Al a Le u 225 Ala T yr Glu Gly Arq 305 Se r Arq Al a Gly Glu 385 Gly Al a Glu Cys Arg Se r Giu Val1 195 Al a ValI Pro Ala Lys 275 Pro Tyr Ser Se r Ala 355 Arg ValI Pro Le u Le u 435 Val Glu Val1 Leu 180 Arg Ala Se r ValI Ala 260 His Gl y Le u Al a Gln 340 His ValI Gly T rp Arg 420 Leu Ala Leu Met 165 Asn Arg Ser Glu Ala 245 Th r Ser Val1 His Met 325 Leu His Pro Le u Se r 405 Thr Asn Val1 Arg 150 Al a Ala Ser Leu Val 230 Glu Arg Gin T yr Gly 310 Glu Pro His Asp Asn 390 Th r Pro T yr Leu 135 Lys Gly Val1 Trp Gl y 215 Al a Leu I le Gln Val1 295 Ser Glu Le u Le u Al a 375 As n Ser Pro Pro Pro L ys Glu Thr Ala 200 Thr Gly Gly Leu Lys 280 Lys Ala Gly Ly s Ser 360 Thr Thr Gln Le u Gly 440 Val1 Arg Le u Arg 185 Th r ValI Tle Arg Ile 265 Le u Gly Asp Th r Gin 345 Arg Val1 Gly Ile Leu 425 ValI ValI Gly Leu 170 Asp Gly Se r Met Val 250 Pro Pro Ile Al a Asp 330 Arq Gi y Glu Ly s Asn 410 Val1 Ile Ala Arg 155 His Ser Phe Ile Met 235 Val1 Le u Gly Se r Thr 315 Val1 Arg Ser Glu Gin 395 Lys Leu Ile Arq 140 Met Al a Asp Se r Val1 220 Leu Giu Leu Le u Ala 300 Arg Ile Ala Val1 Ile 380 Arg Leu Glu Se r Trp Thr Ala Ala Thr Gly Arg Val 190 Arg Ala 205 Ile Ser Leu Gly Tyr Arg Gln Arg 270 Gln Ala 285 Leu Pro Lys Trp Val Asn Leu Ile 350 Ser Arg 365 Glu Gin Leu Lys Lys Ile Gly Ile 430 Thr Val 445 Le u Arg Al a 175 ValI Le u Gly Val1 Gln 255 Gly Thr Gly Val1 Gly 335 Gly Le u Al a As n Al a 415 Th r Gln Ly s Ile 160 Ile Ile Met His Leu 240 As n Se r Glu Glu Thr 320 Gln Ile Val1 Le u Gly 400 Ser Pro Glu WO 01/00805 Asn Pro Ser Glu Thr Trp Arg Gin Val Asn Ile 450 455 <210> 303 <211> 412 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(412) <223> FRXA00456 <400> 303 ctcaccaacc cggagatcgt cacagcggtg ctaacggatc tcgtcqcaqa cttttgctca ttgccctagg tgtacttggt PCT/IBOO/00926 ctg Le u cga Arg gcg Ala gtg Vali aat Asn acc Thr ggg Gly atg Met gt t Val att Ile gt g Val t cq Se r cgc Arq gtq Vai gga Gly caa Gin cgt Arg ttg Le u aat As n t tg Le u ctg Leu gt a ValI qaa GI u gt g Val1 gt g Vali agc Se r ctt Leu gtg Val1 ttg Leu act Thr gct Al a gta ValI gct Al a 30 gca Al a gtg Val1 gt c ValI ttq Leu gCC Ala 15 acc Thr qa a Glu gg t Gly acc Thr 9gc Gi y 95 9CC Al a a cg Thr qa t Asp gct Ala cga Arg 80 att Ile atgcctagct tatgqcqtgc gtg ctg cag gca ctg Val Leu Gin Ala Leu 1 ata ctt gag gga aac Ile Leu Giu Gly Asn ttg gqt ttq ggg gtg Leu Gly Leu Gly Val cta gqc cag cat tat Leu Giy Gin His Tyr gcq ctq gtg cct gga Ala Leu Val Pro Gly qcc agc aat gat ctc Ala Ser Asn Asp Leu gtt ccq atg gtc acc Val Pro Met Val Thr 100 115 163 211 259 307 355 403 <210> 304 <211> 104 <212> PRT <213> Corynebacterium glutamicum <400> 304 Val Leu Gin Ala Leu Leu Ala Ile Met Val Ser Leu Ser Val Ala Ala 1 5 10 Ile Leu Glu Gly Asn Arg Ala Leu Val Gly Leu Leu Leu Ala Thr Thr WO 01/00805 PCTIIBOO/00926 25 Leu Gly Leu Gly Val Ala Gin Trp Ile Gin Lys Vai Val Ala Giu Asp 40 Leu Giy Gin His Tyr Val His Giu Val Arg Arg Glu Leu Val Gly Ala 55 Ala Leu Val Pro Gly Asn Thr Ala Ser Leu Gly Val Thr Val Thr Arg 70 75 Ala Ser Asn Asp Leu Thr Ala Val Arq Asn Trp Val Ala Leu Gly Ile 90 Val Pro Met Val Thr Gly Leu Pro 100 <210> 305 <211> 1040 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1017) <223> FRXA00459 <400> 305 aggcgtggct gttactgtgc cactqctcat gtg tgt acc cgt gct qcc ggt qqt 54 Val Cys Thr Arg Ala Ala Gly Gly 1 ggc gcq gtg act ttq aaa aga qca cgt gaa cta cqc aaa aaa cgt gga 102 Gly Ala Val Thr Leu Lys Arq Ala Arg Glu Leu Arg Lys Lys Arq Gly 15 cgc atg gct gcg cgg atc gca gat tct gtc atg qct gga gaa tta ctg 150 Arg Met Ala Ala Arg Ile Ala Asp Ser Val Met Ala Gly Glu Leu Leu 30 35 cac gca aca gga gca ata gac cqt gag ctc aat qca gtc acc cga gat 198 His Ala Thr Gly Ala Ile Asp Arg Giu Leu Asn Ala Val Thr Arg Asp 50 tcc gac cga gtg gtq ata qct gct gta aga cgt tcc tgg qcc acc ggt 246 Ser Asp Arg Val Val Ile Ala Ala Val Arg Arg Ser Trp Ala Thr Gly 65 ttt agc cgc gca ttq atg gcc atg gca gcc tcg ctt ggc act qtc agc 294 Phe Ser Arg Ala Leu Met Ala Met Ala Ala Ser Leu Gly Thr Val Ser 80 att gtg att tct ggc cac ctg gaa gta agt gag qtt gcg qqa ata atg 342 Ilie Val Ile Ser Gly His Leu Giu Val Ser Glu Val Ala Gly Ile Met 95 100 atg ctt ctt gqc gtt ctt qcc act cca qtt qca gaa ctt ggc cqc gtg 390 Met Leu Leu Gly Val Leu Ala Thr Pro Val Ala Glu Leu Gly Arg Val 105 110 115 120 WO 01/00805 WO 0100805PCT/IBOO/00926 gtg gaa tat cgc Val Glu Tyr Arg aat tat aaa gcc Asn Tyr Lys Ala aca cgc atc ctg Thr Arg Ile Leu att cca Ile Pro 135 ctt ctg caa Leu Leu Gin ggq ttg caa Gly Leu Gin 155 ggc tca gaa ttt Gly Ser Giu Phe cac tcc caa caa His Ser Gin Gin aaa cta ccc Lys Leu Pro 150 aaa ggt att Lys Gly Ile gca aca qaa qga Ala Thr Giu Gly ccc ggt gtc tat Pro Gly Val Tyr tcC gcc Ser Ala 170 ctt cct gga gaa Leu Pro Gly Giu cgg Arg 175 atc tac ctc cac Ile Tyr Leu His tct gca gat gcq Ser Ala Asp Ala a cg Thr 185 aqa aaa tqq gtc Arq Lys Trp Val tcg ttg tct gca Ser Leu Ser Ala qaq qaa ggc aca Giu Giu Gly Thr ga t Asp 200 gta ata gtc aac Val Ile Val Asn caa agq ctt tcg Gin Arg Leu Ser ctt cct ttg aaa Leu Pro Leu Lys caa cga Gin Arq 215 cgc gcc ctc Arg Ala Leu tca gta tcg Ser Val Ser 235 gga atc gcc tca Gly Ile Ala Ser cac cac cac tta His His His Leu agc cgt ggt Ser Arg Gly 230 acc gtg gaa Thr Val Giu cgc ctg qtt qgt Arg Leu Val Gly ttq Le u 240 cga qtg ccg gat Arg Val Pro Asp gaa att Giu Ile 250 gag caa gca ctg Glu Gin Ala Leu caa gtt ggt ctg Gin Val Gly Leu aac acc 999 aaa Asn Thr Gly Lys caa cgc ttg aaa aac Gin Arg Leu Lys Asn 265 gga cac ccc tgg Gly His Pro Trp act tcg cag atc Thr Ser Gin Ile aac Asn 280 aaa ctg aaa att Lys Leu Lys Ile agc gcc acc ctt Ser Ala Thr Leu acc cca ccg ctt Thr Pro Pro Leu ttg gta Leu Val 295 ctt gaa ggc Leu Giu Gly atc tcc acc Ile Ser Thr 315 acc cct gaa aac Thr Pro Giu Asn ctc aac tat ccc Leu Asn Tyr Pro gga gtg atc Gly Val Ile 310 caa qtq aac Gin Val Asn gtt cag gag aac Val Gin Glu Asn tcc gaa aca tgg Ser Glu Thr Trp 966 1014 1040 atc taatctagaa acatggcagg acg Ile <210> 306 <211> 329 <212> PRT <213> Corynebacterium glutamicum WO 01/00805 PCTIIBOO/00926 <400> 306 Val Cys Thr Arg Ala Ala Gly Gly Gly Ala Val Thr Leu Lys Arg Ala 1 5 10 Arg Giu Leu Arg Lys Lys Arq Gly Arg Met Ala Ala Arg Ile Ala Asp 25 Ser Val Met Ala Gly Glu Leu Leu His Ala Thr Gly Ala Ilie Asp Arg 40 Glu Leu Asn Ala Val Thr Arg Asp Ser Asp Arg Val Val Ile Ala Ala 55 Val Arg Arg Ser Trp Ala Thr Giy Phe Ser Arg Ala Leu Met Ala Met 70 75 Ala Ala Ser Leu Gly Thr Val Ser Ile Val Ile Ser Gly His Leu Giu 90 Val Ser Glu Val Ala Gly Ile Met Met Leu Leu Gly Val Leu Ala Thr 100 105 110 Pro Vai Ala Giu Leu Gly Arg Val Val Giu Tyr Arq Gin Asn Tyr Lys 115 120 125 Ala Ala Thr Arg Ilie Leu Ile Pro Leu Leu Gin Arg Gly Ser Glu Phe 130 135 140 Lys His Ser Gin Gin Lys Leu Pro Gly Leu Gin Ala Thr Giu Gly Ile 145 150 155 160 Pro Gly Val Tyr Val Lys Gly Ilie Ser Ala Leu Pro Gly Giu Arg Ile 165 170 175 Tyr Leu His Gly Ser Ala Asp Ala Thr Arg Lys Trp Val Thr Ser Leu 180 185 190 Ser Ala Met Giu Giu Gly Thr Asp Val Ilie Vai Asn Gly Gin Arg Leu 195 200 205 Ser Gin Leu Pro Leu Lys Gin Arg Arg Ala Leu Ile Gly Ilie Ala Ser 210 215 220 Ala His His His Leu Ser Arg Gly Ser Val Ser Arg Leu Val Gly Leu 225 230 235 240 Arg Val Pro Asp Ala Thr Val Giu Giu Ilie Giu Gin Ala Leu Giu Gin 245 250 255 Vai Gly Leu Asn Asn Thr Gly Lys Gin Arg Leu Lys Asn Gly-Gly His 260 265 270 Pro Trp Ser Thr Ser Gin Ilie Asn Lys Leu Lys Ile Ala Ser Ala Thr 275 280 285 Leu Arq Thr Pro Pro Leu Leu Val Leu Giu Gly Ile Thr Pro Giu Asn 290 295 300 Leu Leu Asn Tyr Pro Gly Val Ilie Ile Ser Thr Val Gin Giu Asn Pro 305 310 315 320 WO 01/00805 WO 0100805PCTIBOO/00926 Ser Glu Thr Trp Arg Gin Val Asn Ilie 325 <210> <211> <212> <213> <220> <221> <222> <223> 307 771

DNA

Corynebacterium giutamicum

CDS

(101)..(748) RXN01604 <400> 307 ctctcatgtt gttgtcctct agttgacagc gggggtggtg gtggtcctaa aatagcctac gataactgat agtgtttcct ccacttacqg aagaagatac atg aat acc cct ctt Met Asn Thr Pro Leu ttg aga agc tct Leu Arg Ser Ser ggg Giy ctc tcc atc cgc Leu Ser Ilie Arq aca ccc ttc gcc Thr Pro Phe Ala gat gtt Asp Vai gag ata gct Giu Ile Ala tcc caa tcc Ser Gin Ser gac agc gga ctc Asp Ser Gly Leu ttg ctg agc acc Leu Leu Ser Thr ggg cgc gaa Gly Arg Giu atg cgc gcc Met Arq Ala 211 259 agt tcc ttt tct Ser Ser Phe Ser gta ctt tcc ggc Val Leu Ser Gly tcc acc Ser Thr gga acc atc gaa Gly Thr Ile Giu aac ggc gaa ccc Asn Gly Giu Pro atc Ile aag gca acc aag Lys Ala Thr Lys gcc aag cat gtg Ala Lys His Val ttg gcg gqc atc Leu Ala Gly Ile cc t Pro gaa atc gat tca Gl Ile Asp Ser gag cga ctt gtc Giu Arg Leu Val gtg cgc acc gtt Val Arg Thr Val gt c Val1 cgt gaa caa ctc Arg Giu Gin Leu gcc tgg Ala Trp 100 tca agc cct Ser Ser Pro tgg tac ctg atg gtg ccc agg gat att agt gat tcg gga Trp Tyr Leu Met Val Pro Arg Asp Ile Ser Asp Ser Gly 105 110 115 cgg tgg gtt gac gtc gaa aag Arg Trp Val Asp Val Giu Lys 120 ctt ggc ctg aac Leu Gly Leu Asn aac cct aaa Asn Pro Lys acc tta Thr Leu 135 atc ggc gac ctc agc gtg ctc gag cgt Ile Gly Asp Leu Ser Val Leu Giu Arg 140 aag ctg cgc atc Lys Leu Arg Ile gcg Al a 150 ctg gcg ctg ctg Leu Ala Leu Leu cgg cca gag gcg Arg Pro Giu Ala ctg ttg gtc gtg Leu Leu Val Val ga t Asp 165 WO 01/00805 PCTIBOOIOO926 gat ccc gat caa gtg cgc agc atg gaa ttg cgt gcg gag gtg ttg cac -643 Asp Pro Asp Gin Val Arg Ser Met Giu Leu Arg Ala Giu Val Leu His 170 175 180 gca ttg aaa qgc gtt gca gag gat ctc cct gtg qtc gtg gta tcc acc 691 Ala Leu Lys Gly Val Ala Glu Asp Leu Pro Val Val Val Val Ser Thr 185 190 195 aac cca gat ttt gat tcc ttg gcc gat acc gct ttg acc att acg ggg 739 Asn Pro Asp Phe Asp Ser Leu Ala Asp Thr Ala Leu Thr Ile Thr Gly 200 205 210 gct gga aac taatqgcatt tttacacttt ggc 771 Ala Gly Asn 215 <210> 308 (211> 216 <212> PRT <213> Corynebacterium glutamicum <400> 308 Met Asn Thr Pro Leu Leu Arg Ser Ser 1 Pro Se r Gly Ile Glu Glu Ile Asn Phe 145 Leu Ala Val1 5 Val Glu Al a Lys Le u Trp Gly Lys Ile Asp 165 His Th r Gi u Se r Se r Le u Giu Se r Arg Thr Al a 150 Asp Ala Asn Al a Ser Gi y Lys Leu Pro Val1 120 Ile Ala Asp Lys Asp Gi y Asp Se r Ile Val Thr Tyr Val1 Asp Le u Val1 170 Val Asp Ile Le u Le u Asn Ala Thr Val His 125 Val1 Pro Met Asp Ala WO 01/00805 PCT/IB00100926 195 200 205 Leu Thr Ile Thr Gly Ala Gly Asn 210 215 <210> 309 <211> 629 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <222> <223> FRXA01604 <400> 309 gac aca ccc ttc qcc gat gtt gag ata gct cca gac agc gga ctc act 48 Asp Thr Pro Phe Ala Asp Val Glu Ile Ala Pro Asp Ser Gly Leu Thr 1 5 10 ttg ctg agc acc 999 cgc gaa tcc caa tcc agt tcc ttt tct ttq gta 96 Leu Leu Ser Thr Gly Arg Giu Ser Gin Ser Ser Ser Phe Ser Leu Val 25 ctt tcc ggc cgc atg cgc gcc tcc acc gga acc atc gaa tta aac ggc 144 Leu Ser Gly Arg Met Arg Ala Ser Thr Giy Thr Ilie Giu Leu Asn Giy 40 gaa ccc atc aag gca acc aag ctg gcc aag cat qtq qct ttg gcg ggc 192 Giu Pro Ilie Lys Ala Thr Lys Leu Ala Lys His Val Ala Leu Ala Giy 55 atc cct gaa atc gat tca ctc gag cga ctt gtc act gtg cgc acc gtt 240 Ile Pro Giu Ile Asp Ser Leu Giu Arg Leu Val Thr Val Arg Thr Val 70 75 gtc cgt gaa caa ctc 9CC tgg tca agc cct tgg tac ctg atg gtg ccc: 288 Val Arg Glu Gin Leu Ala Trp Ser Ser Pro Trp Tyr Leu Met Vai Pro 90 agg gat att agt gat tcg gga cgg tgg gtt gac gtc gaa aag cat ctt 336 Arg Asp Ilie Ser Asp Ser Giy Arg Trp Val Asp Val Giu Lys His Leu 100 105 110 ggc ctg aac ctg aac cct aaa acc tta atc ggc gac ctc agc gtg ctc 384 Giy Leu Asn Leu Asn Pro Lys Thr Leu Ilie Gly Asp Leu Ser Val Leu 115 120 125 gag cgt ttt aag ctg cgc atc gcg ctg gcg ctg ctg gcg cgg cca gag 432 Giu Arg Phe Lys Leu Arg Ilie Ala Leu Ala Leu Leu Ala Arg Pro Giu 130 135 140 gcg caa ctg ttg gtc gtq gat gat ccc gat caa gtg cgc agc atg gaa 480 Ala Gin Leu Leu Val Val Asp Asp Pro Asp Gin Val Arg Ser Met Giu 145 150 155 160 ttg cgt gcg gag qtg ttg cac gca ttg aaa qgc qtt gca gag gat ctc 528 Leu Arg Ala Giu Val Leu His Ala Leu Lys Gly Val Ala Glu Asp Leu 165 170 175 WO 01/00805 PCTIIBOO/00926 cct gtg gtc gtg qta tcc acc aac cca gat ttt gat tcc ttg gcc gat 576 Pro Val Val Val Val Ser Thr Asn Pro Asp Phe Asp Ser Leu Ala Asp 180 185 190 acc gct ttg acc att acg ggg gct gga aac taatggcatt tttacacttt 626 Thr Ala Leu Thr Ile Thr Gly Ala Gly Asn 195 200 ggc 629 <210> 310 <211> 202 <212> PRT <213> Corynebacterium glutamicum <400> 310 Asp Thr Pro Phe Ala Asp Val Glu Ile Ala Pro Asp Ser Gly Leu Thr Leu Ser Pro Pro Arg Asp Leu Arg 130 Gin Arg Val1 Ser Gly Ile Glu Glu Ile Asn 115 Phe Le u Ala Val Ser Ser Leu Glu Se r Ar g Th r 120 Al a Asp Al a Asn Gin Thr Al a Arg Ser Trp 105 Le u Leu Pro Le u Pro 185 Ser Gly Lys Leu Pro Vali Ilie Ala Asp Ly s 170 Asp Se r Thr His Val T rp Asp Giy Leu Gin 155 Gly Leu Asn Ala Thr Val1 His Val Pro Met Asp 175 Val1 Giy Gi y Val Pro Leu Leu Giu Giu 160 Le u Phe Asp Ser Leu Ala Asp 190 Thr Ala Leu Thr Ile Thr Gly Ala Gly Asn 195 200 <210> 311 <211> 2262 <212> DNA <213> Corynebacterium glutamicum WO 01/00805 WO 0100805PCTIBOO/00926 <220> <221> CDS <222> (101)..(2239) <223> RXNOZ.547 <400> 311 ggggccgtcg ataaqcgaag aacaattagc actgcgcgcg ctgqtctgac gcattggtta aggggctgc qgtccggcgc cggaatcggc ggaggagctt ttg gag Leu Glu ctc aac aac Leu Asn Asn gct Ala gaa Glu gag Giu agt Ser ga t Asp gat Asp gg t Gly Cgg Arg gg C Gly tc Se r 150 gta Val1 cgg Arg gca Ala aaa Lys att Ilie Gly aat Asn gct Ala t gg Trp 120 g tg Val1 atc Ile caa Gin Ct 9 Le u gg t Gly gcg Ala gag Gi u gtg Val1 aa t Asn gcq Ala 105 gta Val1 aca Th r cg a Ar g ga a Glu acc Thr cag Gin qc9 Ala tac T yr a gc Ser g cg Ala gaa Glu aat Asn a cg Th r aaa Lys tta Le u 170 Val1 a gg Arg gat Asp cgt Arg C cg Pro 75 ga a Giu atg Met gcc Ala ggg Gly gt t Val1 155 99 C Gly ga t Asp aat As n caa Gin t tg Le u c9g Arg t gg Trp ttg Le u aaa Lys gag Giu 140 ga t Asp aag Lys gag Glu gta Val gag Glu 45 ctg Leu ga t Asp gaa Glu gt t Val1 cat His 125 tac T yr gtt Val1 cg t Arg tat Tyr gag Glu ctg Leu ca g Gin atg Met

CCC

Pro CC t Pro 110 ttg Le u aaa Lys gt g Val1 ttt Phe ccg Pro 15 ga C Asp t ct Ser gtg Val1 ccc Pro gtt Val1 95 cat His gca Ala acc Th r gag Gi u aat Asn 175 gcg Ala cg a Arg tct Se r cgg Arq ttt Phe 80 gtg Val ggg Gly gcg Ala t cg Ser t cg Ser 160 att Ile 1 gcg Al a acc Thr ttg Le u ga a Gi u gcc Al a ca g Gln t tg Le u ct g Leu cg t Arg 145 ccg Pro cgg Arg agg Arg cg t Arg agt Ser aat Asn ggt Gly cg C Arg ttg Leu ctg Leu 130 ttt Ph e t tt Phe t gc Cys g cg Ala gtt Val ggc Gly tgt Cys ctg Le u t tg Leu 100 cgg Arg ttc Phe gcg Al a ga t Asp cgc Arg 180 ctt Le u ga t Asp agc Se r ca g Gin att Ilie ggt Gly gtt Val1 aac As n ga t Asp tgg Trp 165 act Th r 115 163 211 259 307 355 403 451 499 547 595 643 691 cct gag gaa ttg tcg gcg ctg ggg cca cgc gat cag ggc gtg acc att Pro Giu Glu Leu Ser Ala Leu Gly Pro Arq Asp Gin Gly Val Thr Ile WO 01/00805 WO 0100805PCTIBOO/00926 ttg ggt gtg Leu Gly Val 200 tgg gaa aaa Trp Glu Lys 215 ttg ggt tcc Leu Gly Ser 230 aaa gct ggc Lys Ala Gly aac cgc gct Asn Arg Ala gaa att ttg Glu Ile Leu 280 qcg gcg att Ala Ala Ile 295 cca gag gcc Pro Glu Ala 310 ctc gcg agg Leu Ala Arg gtg gcg tcg Val Ala Ser gaa agc ctg Giu Ser Leu 360 gag aaa cta Giu Lys Leu 375 gat gag cag Asp Giu Gin 390 gcc aat gag Ala Asn Glu ttg cgc agc Leu Arg Ser gcc gaa cct cga aaa ttt Arg gat Asp acc Th r aaa Lys gag Gi u 265 aaa Lys gct Al a acc Thr gt t Val1 a tq Met 345 c cg Pro ttt Phe acc Thr gca Ala tat Tyr 425 gag Lys ga t Asp aat Asn gca Ala 250 c tg Le u gtg Val1 gag Giu gag Glu tct Ser 330 aac Asn gtt Val ctt Le u att Ile gaa Giu 410 att Ile ttt Phe cg C Arg ga t Asp 235 gt t Val cgg Arg t cg Se r ctg Leu ctt Leu 315 gac Asp ctg Leu g cg Ala gcc Ala gcg Ala 395 ctt Le u gaa Glu qtt gcg Ala cga Arg 220 gcc Ala gt g Val gaa Glu tgg Trp gac Asp 300 tcc Se r ttg Le u aaa Ly s gag Giu aac Asn 380 ct g Leu cg a Arg acg Thr gg t Ca g Gin 205 aa g Lys aa g Lys ca g Gin ctt Le u gct Ala 285 cga Arg g cg Ala ctt Leu C gc Arg g tt Val1 365 acC Thr cgc Arg cg t Arg t gg Trp gag ca g Gin Ct g Le u g tg Val gca Al a gaa Giu 270 ca g Gin ttg Leu cgg Arg gtc Val gcc Ala 350 tct Se r cg c Arg gag Glu tgt Cys cct Pro 430 gcc aca Thr ggg Gly ga a Giu gct Al a 255 cgg Arg att Ile ctg Le u cat His gca Ala 335 gaa Glu ga a Glu cgg Arg ga t Asp gaa Glu 415 gcg Ala atc ggc Gly ga t Asp a cg Thr 240 qa t Asp cag Gin ga t Asp gaa Glu gag Glu 320 gc t Ala act Th r gaa Glu gtt Val1 ctg Leui 400 aac As n aac As n aac ga t Asp cg t Arg 225 ctt Le u aat Asn tat Tyr gt g ValI gag Giu 305 gcg Ala cag Gin gaa Glu atc Ile cac His 385 ga C Asp ca a Gin cgc Arg cgc ccg Pro 210 tcc Se r cgg Arg cgc Arg caa Gln ga a G lu 290 ctg Leu g cg Ala agt Se r ttg Leu gcg Al a 370 gcc Ala aaa Lys att Ile gct Ala ctc acg acg Thr Thr aca tac Thr Tyr gaa acc Giu Thr att gct Ile Ala 260 gct tCg Aia Ser 275 tca gcc Ser Ala aac aac Asn Asn aag cag Lys Gin gag gaa Glu Glu 340 aaa cgg Lys Arg 355 cgg gaa Arg Glu gcc aac Ala Asn caa atc Gin Ile gtt ggc Vai Gly 420 gac tta Asp Leu 435 ggc gag cg t Arg cg t Arg gtg Val.

245 gca Ala caa Gin ga c Asp act Thr a cg Thr 325 acc T hr ctc Le u gtg ValI gtg Val gat Asp 405 att Ile caa Gin ctt 739 787 835 883 931 979 1027 1075 1123 1171 1219 .1267 1315 1363 1411 1459 WO 01/00805 WO 0100805PCT/IB00100926 Ala Giu Pro Giu Phe Val Gly Giu Ala Ile Asn Arg Leu Gly Giu Leu cgc Arg aac As n 470 ga t Asp ttg Le u cg t Arg gcc Al a gcc Al a 550 gac Asp cac His aa c As n ctg Le u ccc Pro 630 ttc Phe cac His ca a Gin agc Se r 455 gag Glu gcq Al a gcg Ala ga t Asp gc t Al a 535 cg t Arg tcc Se r gt t Val1 acc Thr gtg Val1 615 gqc Gly gac Asp agc Se r a cc Thr 440 ga t Asp atg Met cgc Arg cag Gin caa Gin 520 acc Thr tat T yr g cc Al a cgc Arg tac T yr 600 ttt Phe g cc Ala cgc Arg ttc Phe ctc Leu cg t Arg t cc Se r cg g Ar g tcg Se r 505 agt Ser agc Se r gcg Al a ga c Asp ttc Phe 585 gt c Val1 ttc Phe cat His gcc Al a qqc Gly 665 gg C Gly 445 450 ttg Leu acc Th r gaa Gi u 490 ga a Giu gg t Gly ggt Gi y ctg Leu gcc Al a 570 atc Ile gac Asp t gc Cys tat T yr ga c Asp 650 ttc Phe ga t Asp gca Al a cga Arg 475 atc Ilie ttc Phe ccq Pro ga c Asp atc Ile 555 cgc Arg ggc Gly tcc Ser ctc Leu ccc Pro 635

CCC

Pro cac His tat Tyr ga a Giu 460 aac As n ga g Glu aac As n att Ile ctg Leu 540 gct Ala tgg Trp ctc Leu gca Ala gcc Al a 620 acc Thr gcc Ala atg Met gt c Val1 ttc Phe CtC Le u ga g Giu gaa Giu gtg Val 525 gga Gly ga a Giu cgc Arg gag Glu t ca Se r 605 gct Ala tac Tyr ttC Phe gtg Val1 ggc Gly a cg Th r ggc Gly cg C Arg ggt Gly 510 a gg Arg acc Th r a tc Ile aac Asn cgc Arg 590 ctt Le u gcc Ala g cc Ala acc Thr ctc Le u 670 t CC Se r gcc Ala caa Gin atc Ile 495 cg C Arg ga a Glu agt Se r att Ile acc Thr 575 gat Asp t ca Ser ttg Le u acc Thr cgc Arg 655 g cg Al a acc Thr aaa Lys atc Ile 480 gag Glu ttc Phe ttc Phe acc Thr tCC Ser 560 gtt Val1 tcC Se r ggc Gly cgc Arg gtc Val1 640 caa Gin acc Thr atc Ile ttc Phe 465 t cg Se r ccg Pro ctg Leu ca g Gin gag Glu 545 aaa Lys ct a Leu ga c Asp gg a Gi y tac Tyr 625 att Ilie acc Thr C cg Pro gtg Val1 cta Leu cgg Arg atc Ile caC His cag Gin 530 aaa Lys ctC Le u ga C Asp ggc Gly caa Gin 610 cag Gin ctg Leu atg Met ctg Leu gtc Val ggg ctc Gly Leu cgt cta Arg Leu aac gcc Asn Ala 500 atc gac Ile Asp 515 aaa ctt Lys Leu caa gcc Gin Ala gcc tcc Ala Ser acc cgc Thr Arg 580 gca acc Ala Thr 595 gcc cag Ala Gin cta gcc Leu Ala gac gaa Asp Giu aac gtC Asn Val 660 aaa ctt Lys Leu 675 agc tac Ser Tyr a tg Met Cgt Arg 485 tcC Se r atc Ile ga t Asp ttc Phe cac His 565 cgc Arg gt C Val1 aag Lys ga a Glu gcc Al a 645 ttC Phe atc Ile acc Thr 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 2131 2179 WO 01/00805 PCT11BOO100926 680 685 690 qaa aaa cca aac gcc cag ggc gca, att cag ggc aat tcc agt ttc tct 2227 Giu Lys Pro Asn Ala Gin Gly Ala Ile Gin Giy Asn Ser Ser Phe Ser 695 700 705 agg atc gag aaa taacatgcca ttgtttatcg acg 2262 Arg Ilie Glu Lys 710 <210> 312 <211> 713 <212>' PRT <213> Corynebacterium giutamicum <400> 312 Leu Giu Leu Asn Asn Ala Ala Arg Leu Thr Val Asp Giu Tyr Pro Ala 1 5 10 Ala Arg Giu Ala Leu Giu Ser Ala Gly Gin Arg Asn Val Glu Asp Arg 25 Thr Arg Ala Val Asp Giu Phe Lys Ala Ala Asp Gin Giu Leu Ser Ser 40 Leu Ser Lys Gly Ser Ser Asn Ile Giu Tyr Arg Leu Leu Gln Val Arg 55 Glu Asn Leu Cys Gin Asp Leu Gly Val Ser Pro Arcj Asp Met Pro Phe 70 75 Ala Gly Giu Leu Ile Asp Pro Asn Asn Ala Glu Trp Glu Pro Val Val 90 Gin Arg Ile Leu Gly Gly Phe Ala*Ala Giu Met Leu Val Pro His Gly 100 105 110 Leu Leu Pro Arg Val Arg Asp Trp Val Asn Ala Lys His Leu Ala Ala 115 120 125 Leu Leu Lys Phe Asn Gly Val Val Thr Thr Gly Glu Tyr Lys Thr Ser 130 135 140 Arg Phe Pro Ala Asp Ser Leu Ile Arg Lys Val Asp Val Val Glu Ser 145 150 155 160 Pro Phe Arg Asp Trp Val Asn Gin Glu Leu Gly Lys Arg Phe Asn Ile 165 170 175 Arg Cys Val Arg Thr Pro Glu Giu Leu Ser Ala Leu Gly Pro Arg Asp 180 185 190 Gin Gly Val Thr Ile Leu Gly Val Arg Lys Phe Ala Gin Gin Thr Gly 195 200 205 Asp Pro Thr Thr Arg Trp Giu Lys Asp Asp Arg Arg Lys Leu Gly Asp 210 215 220 Arg Ser Thr Tyr Arq Leu Gly Ser Thr Asn Asp Ala Lys Val Glu Thr 225 230 235 240 WO 01/00805 WO 0100805PCT1BOO/00926 Le u Asn Tyr Val1 Giu 305 Al a Gin Giu Ile His 385 Asp Gin Arg Arg Phe 465 Se r Pro Leu Gin Glu 545 Thr Ala 260 Se r Ala Asn Gin Giu 340 Arg Glu Asn Ilie Gly 420 Le u Giu Le u Le u Ala 500 Asp Le u Al a Val1 245 Ala Gin Asp Thr Thr 325 Thr Leu Val Val Asp 405 Ile Gin Leu Met Arcj 485 Ser Ilie Asp Phe Lys Asn Glu Ala Pro 310 Le u Val1 Glu Glu Asp 390 Al a Le u Al a Arg As n 470 Asp Leu Arg Ala Ala 550 Lys Glu 265 Lys Ala Thr Val1 Met 345 Pro Phe Thr Al a Tyr 425 Giu Arg Ser Arg Ser 505 Ser Se r Ala Ala 250 Leu Val1 Giu Giu Ser 330 As n Val Le u Ile Giu 410 Ilie Phe Le u Thr Glu 490 Gi u Gi y Gly Le u Val Arg Ser Le u Le u 315 Asp Leu Al a Ala Al a 395 Leu Giu Val1 Al a Arg 475 Ile Phe Pro Asp Ile 555 Val1 Giu Trp Asp 300 Se r Le u Lys Giu Asn 380 Le u Arq Th r Gly Giu 460 Asn Giu Asn Ile Leu 540 Ala Gin Aia Leu Giu 270 Ala Gin 285 Arg Leu Ala Arg Leu Val Arg Ala 350 Val Ser 365 Thr Arg Arg Giu Arq Cys Trp Pro 430 Glu Ala 445 Phe Thr Leu Gly Giu Arg Glu Gly 510 Val Arg 525 Gly Thr Giu Ile Ala Asp 255 Arg Gin Ile Asp Leu Glu His Glu 320 Ala Ala 335 Giu Thr Glu Giu Arq Val Asp Leu 400 Glu Asn 415 Ala Asn Ile Asn Ala Lys Gin Ile 480 Ile Glu 495 Arg Phe Glu Phe Ser Thr Ile Ser 560 WO 01/00805 Lys Leu Ala Ser His Asp Ser Ala 565 Leu Asp Thr Arg Arg His Val Arg 1 580 Asp Giy Ala Thr Val Asn Thr Tyr 595 600 Gly Gin Ala Gin Lys Leu Val Phe 1 610 615 Tyr Gin Leu Ala Glu Pro Gly Ala 625 630 Ile Leu Asp Giu Ala Phe Asp Arg 1Z 645 Thr Met Asn Val Phe His Ser PheC 660 Pro Leu Lys Leu Ile Gin Thr LeuC 675 680 Val Val Ser Tyr Thr Giu Lys Pro .1 690 695 Asn Ser Ser Phe Ser Arg Ile Giu 1 705 710 <210> 313 <211> 2147 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (2124) <223> FRXA02547 <400> 313 gct gcg cgg ctg acc gtg gat gag I Ala Ala Arg Leu Thr Val Asp Glu 1 5 gaa tct gca ggt cag agg aat gta Giu Ser Ala Gly Gin Arg Asn Vai gag ttc aaa gcg gcg gat caa gag Giu Phe Lys Ala Ala Asp Gin Giu 40 agt aat att gag tac cgt ttg ctg Ser Asn Ile Giu Tyr Arg Leu Leu 55 gat ttg ggc gtg agc ccg cgg gat Asp Leu Gly Val Ser Pro Arg Asp 1 70 Arg Gly Se r Le u Pro 635 Pro His Tyr Gin gcg Al a cga Arg tct Se r cg g Arg ttt Ph e Trp Leu Al a Ala 620 Thr Ala Met ValI Gly 700 gcg Ala acc Th r t tg Le u gaa Giu gcc Ala Arg Giu Se r 605 Ala Tyr Phe Val Gly 685 Al a a gg Ar g cgt Arg agt Ser aat As n qg t Gly As n Arg 590 Leu Ala Ala Thr Le u 670 Se r Ile ga a Glu g cg Ala aaa Lys ttg Leu gag Glu Thr 575 Asp Ser Leu Thr Arg 655 Ala Thr Gin gcg Ala gt t Val ggC Gly tg t Cys ctq Le u PCTIIBOOIOO926 Val Ser Gly Arg ValI 640 Gin Th r I le Gly WO 01/00805 WO 0100805PCT/IBOO/00926 gat Asp ggt Gly cgg Arg ggc Gi y tcc Se r 145 gta Val1 cct Pro ttg Le u tgg Trp ttg Leu 225 aaa Lys aac Asn ga a Giu gcg Ala cca Pro 305 ccg Pro ttt Phe gat Asp gtg Val 130 ctg Leu aat Asn gag Giu ggt Gly gaa Glu 210 ggt Gi y gct Ala cgc Arg att Ile gcq Al a 290 gag Glu aat Asn gct Ala t gg T rp 115 gtg Val1 atc Ile caa Gin ga a Glu gtg Val1 195 aaa Ly s tcc Ser Gi y gc t Ala t tg Leu 275 att Ile gcc Ala aat As n gc g Ala 100 gta Val a ca Thr cga Ar g gaa Glu ttg Le u 180 cg a Arg ga t Asp acc Th r aaa Lys gag Glu 260 aaa Ly s gct Al a acc Th r gcg Al a gaa Giu aat Asn acq Th r aaa Lys tta Le u 165 tcg Ser aaa Lys ga t Asp aat As n gca Ala 245 ctg Le u gtg Val gag Glu gag Glu gaa Glu atg Met gcc Al a ggg Gly gtt Val 150 ggc Gly gcg Ala ttt Phe cg c Arg ga t Asp 230 gtt Val cgg Arg t cg Se r ct g Le u ctt Le u 310 tgg Trp ttg Leu aaa Lys gag Glu 135 ga t Asp aag Lys ct g Le u g cg Ala cga Arg 215 gcc Al a gtg Val1 gaa Glu tgg Trp ga c Asp 295 tcc Se r gaa Gi u gt t Val1 cat His 120 tac Tyr gt t ValI cg t Arg 9g9 Gly ca g Gin 200 aag Lys aag Lys ca g Gin ctt Leu gc t Ala 280 cga Arg g cg Ala gt t Val1 90 cat His gca Ala acc Th r gag Gi u aat As n 170 cgc Arg aca Thr ggg Gly gaa Glu gct Ala 250 cg g Arg att Ile ctg Leu cat His gtg Val1 ggg Gly g cg Al a tcg Ser t cg Se r 155 att Ile ga t Asp gg C Gly ga t Asp acg Thr 235 ga t Asp ca g Gin gat Asp ga a Gi u gag Glu 315 cgc Arg ttg Le u ctg Leu 125 ttt Phe ttt Phe t gc Cys ggc Gly ccg Pro 205 tcc Ser Cgg Arg cgc Arg caa Gin gaa Glu 285 ctq Le u qcg Ala att Ile C ca Pro 110 aaa Lys ccg Pro cg C Arg gt g Val1 gtg Val1 190 acg Thr aca Thr ga a Glu att Ile gct Ala 270 t ca Ser aac Asn aag Lys t tg Le u cgg Arg ttc Phe gcg Ala gat Asp cg C Arg 175 acc Thr acg Thr tac Tyr acc Thr gct Al a 255 t cg Se r gcc Al a aac Asn ca g Gin ggt Gly gtt Val aac Asn gat Asp t gg Trp 160 act Thr att Ile cgt Arg cgt Arg g tg ValI 240 gca Ala caa Gin ga c Asp act Thr a cg Thr 320 288 336 384 432 480 528 576 624 672 720 768 816 864 912 960 wo 01/00805 WO 0100805PCTJIBOO/00926 ctc Le u qt g Val1 ga a Glu gag Glu ga t Asp 385 gcc Al a ttg Le u gc C Al a cgc Arg aac Asn 465 gat Asp ttg Leu cgt Arq 9CC Ala 9CC Ala 545 ga c tct Se r 325 a ac Asn gtt Val1 ctt Leu att Ile ga a Giu 405 att Ile ttt Phe ttg Le u acc Th r gaa Glu 485 ga a Glu gg t Gly gg t Gly ctg Le u gcc gac Asp ct 9 Le u gcg Ala gcc Ala gcg Ala 390 ctt Leu qaa Glu gtt Val1 9 ca Ala cga Arq 470 atc Ile ttc Phe C C9 Pro gac Asp atc Ile 550 c C)c ttg Leu aaa Lys gag Glu aac Asn 375 ct g Leu cga Arq acg Thr gqt Gly ga a Gi u 455 aa C Asn gag Glu aac Asn at t Ile ct g Le u 535 gc t Al a tgg gtc Val gcc Al a 345 t ct Ser eg C Arg gag Glu tgt Cys c~t Pro 425 gcc Al a acq Thr gg c Gly cg C Arg 99 t Gi y 505 agg Arg acc Thr atc Ile aac gct Al a act Thr gaa Glu gt t Val1 ctg Leu 395 a ac Asn aac Asn aac Asn aaa Lys atc Ile 475 gag Glu ttc Phe ttc Phe a cc Thr tCC Se r 555 gtt a gt Ser ttg Leu g cg Ala 365 gcc Ala aaa Lys att Ile gct Al a ctc Le u 445 cta Leu c gg Arg atc Ile cac His cag Gin 525 aaa Lys ctc Leu ga c gag Glu aaa Lys 350 c9g Arg gcc Al a caa Gin gtt ValI ga c Asp 430 ggc Gly ggg Gly cgt Arg a ac Asn atc Ile 510 aaa Lys caa Gin gcc Ala acc gaa Glu 335 cgg Ar g ga a Glu aac Asn atc Ile Gly 415 tta Le u gag Glu ctc Le u ct a Le u gcc Al a 495 ga c Asp ctt Le u gcc Ala tcc Se r cg c 1008 1056 1104 1152 1200 1248 1296 1344 1392 1440 1488 1536 1584 1632 1680 1728 WO 01/00805 WO 0100805PCTA~BOOI00926 Asp Ser Ala Asp Ala Arg Trp Arg Asn Thr Val Leu Asp Thr Arg Arg 565 570 cac gtt cgc ttc atc ggc ctc gag cgc gat tcc His Val Arg Phe Ile Giy Leu Glu Arg Asp Ser 580 585 aac acc tac gtc gac tcc gca tca ctt tca ggc Asn Thr Tyr Vai Asp Ser Ala Ser Leu Ser Gly 595 600 ctg gtg ttt ttc tgc ctc gcc gct gcc ttg cgc Leu Val Phe Phe Cys Leu Ala Ala Ala Leu Arg 610 615 ccc ggc gcc cat tat ccc acc tac gcc acc gtc Pro Gly Ala His Tyr Pro Thr Tyr Ala Thr Val 625 630 635 ttc gac cgc gcc gac ccc gcc ttc acc cgc caa Phe Asp Arg Ala Asp Pro Ala Phe Thr Arg Gin 645 650 cac agc ttc gqc ttc cac atg gtg ctc gcg acc His Ser Phe Giy Phe His Met Val Leu Ala Thr 660 665 caa acc ctc ggc gat tat gtc ggc tcc acc atc Gin Thr Leu Gly Asp Tyr Val Gly Ser Thr Ile 675 680 qaa aaa cca aac qcc cag gqc gca att cag ggc Giu Lys Pro Asn Ala Gin Gly Ala Ile Gin Gly 690 695 aqq atc gag aaa taacatqcca ttgtttatcg acg Ar Ile Giu Lys 705 <210> 314 <211> 708 <212> PRT <213> Corynebacterium qiutamicum <400> 314 Ala Ala Arg Leu Thr Val Asp Giu Tyr Pro Aia 1 5 10 Giu Ser Ala Gly Gin Arg Asn Val Giu Asp Arg 25 Giu Phe Lys Ala Ala Asp Gin Giu Leu Ser Ser 40 Ser Asn Ile Glu Tyr Arg Leu Leu Gin Val Arg 55 Asp Leu Gly Val Ser Pro Arg Asp Met Pro Phe 70 ga c Asp gga Gly tac Tyr 620 att Ile a cc Thr c cg Pro gt g Vai aat Asn 700 Giy caa Gin 605 ca g Gin ctg Le u at g Met ctg Le u gt c Val1 685 tcc Ser 1776 1824 1872 1920 1968 2016 2064 2112 2147 Aia Arg Glu Ala Thr Arg Aia Vai Leu Ser Lys Gly Giu Asn Leu Cys Ala Gly Giu Leu WO 01/00805 WO 0100805PCTIBOOIOO926 Asp Pro Asn Asn Ala Glu Trp Giu Pro Val Val Gin Ar Ile Leu Gly 90 Gly Phe Ala Ala Giu Met Leu Val Pro His Gly Leu Leu Pro Arg Val 100 105 110 Arg Asp Trp Val Asn Ala Lys His Leu Ala Ala Leu Leu Lys Phe Asn 115 120 125 Gly Val Val Thr Thr Gly Glu Tyr Lys Thr Ser Arg Phe Pro Ala Asp 130 135 140 Ser Leu Ile Arg Lys Val Asp Vai Val Giu Ser Pro Phe Arg Asp Trp 145 150 155 160 Val Asn Gin Giu Leu Gly Lys Arg Phe Asn Ile Arg Cys Val Arq Thr 165 170 175 Pro Giu Giu Leu Ser Ala Leu Gly Pro Arg Asp Gin Gly Val Thr Ile 180 185 190 Leu Gly Val Arq Lys Phe Ala Gin Gin Thr Gly Asp Pro Thr Thr Arg 195 200 205 Trp Glu Lys Asp Asp Arg Arg Lys Leu Gly Asp Arg Ser Thr Tyr Arg 210 215 220 Leu Gly Ser Thr Asn Asp Ala Lys Val Giu Thr Leu Arg Giu Thr Val 225 230 235 240 Lys Al1a Giy Lys Ala Val Val Gin Ala Ala Asp Asn Arg Ile Ala Ala 245 250 255 Asn Arq Ala Giu Leu Arg Giu Leu Giu Arg Gin Tyr Gin Ala Ser Gln 260 265 270 Giu Ile Leu Lys Val Ser Trp Ala Gin Ile Asp Val Giu Ser Ala Asp 275 280 285 Ala Ala Ile Ala Giu Leu Asp Arg Leu Leu Glu Giu Leu Asn Asn Thr 290 295 300 Pro Glu Ala Thr Giu Leu Ser Ala Arg His Giu Ala Ala Lys Gin Thr 305 310 315 320 Leu Ala Arg Val Ser Asp Leu Leu Val Ala Ala Gin Ser Giu Giu Thr 325 330 335 Val Ala Ser Met Asn Leu Lys Arg Ala Glu Thr Glu Leu Lys Arg Leu 340 345 350 Glu Ser Leu Pro Val Ala Giu Val Ser Glu Giu Ile Ala Arq Glu Val 355 360 365 Giu Lys Leu Phe Leu Ala Asn Thr Arq Arg Val His Ala Ala Asn Val 370 375 380 Asp Giu Gin Thr Ile Ala Leu Arg Giu Asp Leu Asp Lys Gin Ile Asp 385 390 395 400 Ala Asn Giu Ala Glu Leu Arq Arg Cys Glu Asn Gin Ile Val Gly Ile WO 01/00805 WO 0100805PCTIIBOO/00926 Leu Arg Ser Tyr 420 Ala Giu Pro Giu 435 Arg Ser Asp Arg 450 Asn Giu Met Ser 465 Asp Ala Arg Arg Leu Ala Gin Ser 500 Arg Asp Gin Ser 515 Ala Ala Thr Ser 530 Ala Arg Tyr Ala 545 Asp Ser Ala Asp His Val Arg Phe 580 Asn Thr Tyr Vai 595 Leu Val Phe Phe 610 Pro Gly Ala His 625 Phe Asp Arq Ala His Ser Phe Gly 660 Gin Thr Leu Gly 675 Giu Lys Pro Asn 690 Ar Ile Glu Lys 705 <210> 315 405 Ile Phe Leu Thr Glu 485 Giu Gly Gly Le u Ala 565 Ile Asp Cys Tyr Asp 645 Phe Asp Giu Val1 Ala Arg 470 Ile Phe Pro Asp Ile 550 Arg Giy Ser Leu Pro 630 Pro His Tyr T rp Giu 440 Phe Leu Giu Giu Val1 520 Gly Giu .Arg Giu Se r 600 Ala Tyr Phe Val1 Gly 680 Arg Arg Phe 460 Se r Pro Leu Gin Giu 540 Lys Le u Asp Gi y Tyr 620 Ile Th r Pro Val Ala Leu 445 Le u Arq Ile His Gin 525 Lys Leu Asp Gi y Gin 605 Gin Leu Met Leu Val1 685 Asp 430 Giy Gly Arg Asn Ile 510 Lys Gin Ala Thr Al a 590 Ala Leu Asp As n Lys 670 Ser Ala Gin Giy 695 Ala Ile Gin Gly Asn Ser Ser Phe Ser 700 WO 01/00805 <211> 1152 <212> DNA <213> Corynebacterium qiutamicum <220> <221> CDS <222> (101)..(1129) <223> RXN02571 <400> 315 tggacaggcc ggggccgcgt acggtgttgq ttgaggtggt PCT[IBOO/00926 qgagggqcgc gtcgaaaagc attgtcgctg gttgttqccq cttttggcag tcgggatqqc gtg gtg gct cta act Val Val Ala Leu Thr caa Gin cg c Arg gcq Ala gg g Gly cqa Arg caa Gin atc Ile ga t Asp ggc Gly gtg Val 150 ttt Phe caa tcc Se r ccc Pro c tg Le u 9gc Gly qgc Gly caa Gin a cg Thr gcc Ala gct Ala ttg Le u 155 cga Arg t gg 9gc Gly qa Gly cgc Arg atc Ile ctc Leu aca Thr atg Met caa Gin gcg Al a 140 gag Giu acg Th r tgg gaa Glu gcc Al a gag Giu tgg Trp cgc Arq gq C Gly ctc Le u ga a Gi u 130 ga c Asp gcg Al a gg a Gi y caa ttg Leu gac Asp ctg Le u gag Glu gca Ala acc Thr tcc Se r 115 tca Ser g cg Ala cgg Arg a cc Thr ca ga a Giu c cg Pro gcc Ala cgc Arg ccc Pro gt c Val1 100 ggt Gly tat Tyr ccq Pro gtg Val1 cg c Arg 180 ca g WO 01/00805 WO 0100805PCTIBOO/00926 Gin Gin Arg Arg Trp Trp Gin Phe Asn Giu Ser Gin Pro Gin Phe ca g Gin ttg Le u gCC Aia 230 gt C Vai ga a Glu ga C Asp gac Asp qcg Ala 310 gaa atc Ile cag Gin 215 aat Asn att Ile cat His gca Al a gtc Val 295 gat Asp acc act Thr 205 ggg Giy ttg Le u aat Asn ca a Gin ccg Pro 285 ggt Gly gga Gly gct Ct 9 Le u qgt Gi y ga t Asp agt Se r 260 caa Gln ga a Glu C9C Arg cat His t 99 Trp ctt Le u caa Gin 245 gaa Giu t9c Cys

CCC

Pro ttt Phe ga t Asp 325 739 787 835 883 931 979 1027 1075 1123 1152 Giu Thr Phe Val Ala Giu Vai Aia Glu 330 tac gcg ata qtg aag gaa atg Tyr Ala Ile Val Lys Giu Met 335 340 999 ctc Gly Leu taggtttctt tggaccaaac cac <210> 316 <211> 343 <212> PRT <213> Corynebacterium giutamicum <400> 316 Val Vai Ala Leu Thr Gin Ile Val Gly 1 5 Arg Giu Leu Giu Lys Arg Tyr Ar9 Glu 25 Thr Ala Asp Pro Arg Ala His Ile Thr 40 Giu Giu Leu Ala Phe Gly Leu Glu Gln 55 Ser Gly Ser Gly Leu Thr Pro Giy Ala Val Met Leu Leu Arg Ala Thr Val Ala Gly Ile Val Pro Ala Gin WO 01/00805 PCT/IB00/00926 Met Trp Glu Arg Val Arg Asn Ile Gly Leu Gly Leu Glu Asn Leu Leu 70 75 Asp Arg Ala Pro Ala Gin Leu Ser Gly Gly Gin Thr Arg Arg Leu Ala 90 Ile Gly Thr Val Ala Ile Leu Glu Ala Pro Thr Met Leu Leu Asp Asp 100 105 110 Pro Leu Ser Gly Leu Asp Thr Ser Ser Arg Ala Gin Leu Ile Thr Met 115 120 125 Leu Glu Ser Tyr Glu Gly Asp Val Ile Val Ala Ala His Lys Arg Trp 130 135 140 Leu Asp Ala Pro Thr Val Tyr Leu Gly Asp Leu Glu Glu Leu Ser Leu 145 150 155 160 Pro Ala Arg Val Glu Phe Ser Gly Pro Ser Arg Thr Phe Ser Ala Ile 165 170 175 Thr Gly Thr Arg Gly Gin Gin Arg Arg Arg Trp Trp Gin Phe Asn Glu 180 185 190 Ser Gin Pro Gin Phe Gin Ile Gly Pro Leu Asp Ile Thr Val Ser Ala 195 200 205 Gly Gin Val Leu Trp Leu Gin Gly Pro Asn Gly Ser Gly Lys Ser Thr 210 215 220 Leu Leu Arg Gly Leu Ala Asn Glu Pro Gly Thr Glu Leu Met Leu Gin 225 230 235 240 Asn Pro Ser Asp Gin Val Ile Asp Ser Thr Val Ala Asn Trp Val Pro 245 250 255 Gly Ser Asn Ser Glu Glu His Pro Leu Asp Leu Ser Gin Arg Glu Leu 260 265 270 Arg Leu Ala Gin Cys Asp Ala Ala Leu Gly Asn Asn Pro Glu Val Leu 275 280 285 Leu Ala Asp Glu Pro Asp Val Gly Leu Asp Val Gly Gly Arg Asn Ala 290 295 300 Ile His Gin Arg Phe Ala Asp Phe Leu Gly Asn Gly Gly Ala Leu Ile 305 310 315 320 Leu Thr Cys His Asp Glu Thr Phe Val Ala Glu Val Ala Glu Tyr Ala 325 330 335 Ile Val Lys Glu Met Gly Leu 340 <210> 317 <211> 1152 <212> DNA <213> Corynebacterium glutamicum WO 01/00805 <220> <221> CDS <222> (1O1)..(ll29) <223> FRXA02571 <400> 317 tggacaggcc ggggccgcgt acggtgiigg ttgaggiggi PCTIIBOO/00926 ggaggggcgc gtcgaaaagc attgtcgcig gttgitgccg ctiiiggcag tcgggatggc gtg gtg gci cia act Val Val Ala Leu Thr ca a Gin cgc Arg gcg Ala 999 Gly cga Arg caa Gin atc Ile ga t Asp ggc Gly gig Val1 150 ttt Phe caa Gin cag aic Ilie tac Tyr cat His ctg Leu aac Asn ctt Leu ita Le u acc Th r gati Asp 135 ta c Ty r icc Se r caa Gin atc gtc Vali Arg aic Ile gaa Giu atc Ilie t cc Ser gag Glu icc Ser 120 gtc Val1 ita Leu ggt Gly cgc Arg 9gc gga Gi y ga a Glu a cc Thr caa Gin ggg Gly ggc Gly gcg Aia 105 i cg S er atc Ile ggg Gly cca Pro cga Arg 185 ccc ccg Pro a cg Thr tac T yr cgc Arg ci c Le u qgg Gly cca Pro cga Arg gi c Vai gati Asp t cg Ser 170 cg c Arg cig icc Ser ccc Pro ci g Leu ggc Gly ggc Gly 75 caa Gin a cg Th r gcc Aia gci Ala i ig Leu 155 cga Arg i gg Trp gat ggc Gly gga Gly cg c Arg aic Ile 60 ci c Leu aca Th r aig Met caa Gin gcg Aia 140 gag Glu a cg Thr igg Trp at icc S er g cg Ala gcg Ala 45 gia Val gag Glu cgg Arg cii Leu cic Leu 125 cac His gag Giu iii Phe caa Gin act ggg Gly gig ValI 30 aca Thr ccc Pro aai As n cgg Arg ci c Leu 110 aic Ile aag Lys ci g Leu i ca Ser tc Phe 190 gt cic Leu 15 aig Met gic Val1 g cg Ala ci g Le u ci g Leu 95 ga c Asp aca Th r cgg Arg icc Ser gcg Al a 175 aac Asn ici a cg Thr cig Leu gcc Al a ca g Gin cia Leu 80 gcg Ala gac Asp aig Met i gg Trp ci g Le u 160 ati Ilie gaa Glu gca 1 cgg Arg a cc Th r gag Giu a tg Met gac Asp aic Ile ccc Pro t 9 Leu ci c Leu 145 cci Pro a ca Th r icc Ser gg i ga a Glu gcc Ala gag Giu igg Trp cgc Arg ggc Gly cic L eu gaa Glu 130 gac Asp 9 cg Ala gga Gly caa Gin caa i ig Leu g ac Asp cig Leu gag Giu 9 ca Ala acc Thr icc Ser 115 i ca S er gcg Ala cgg Arg acc Th r cca Pro 195 gig gaa Giu ccg Pro gcc Ala cg c Arg ccc Pro g ic Vali 100 gg t Gly tai Tyr ccg Pro gig Val cgc Arg 180 cag Gin ci g aaa Lys cg c Arg iii Phe gi c Val1 gcg Alagcc Ala cii Leu gag Giu act Thr gaa Glu 165 gg a Gly iii Ph e i 99 115 163 211 259 307 355 403 451 499 547 595 643 691 739 Gin t tg Leu gco Ala 230 gtc Val ga a Giu 9 ac Asp gao Asp g og Ala 310 gaa Giu WO 01/00805 Ile Gly Pro 200 cag ggt ccc Gin Gly Pro 215 aat gaa ccc Asn Giu Pro att gac tcc Ilie Asp Ser cat ccg ctg His Pro Leu 265 gca gco ctg Aia Ala Leu 280 gtc ggc ott Val Gly Leu 295 gat ttc tta Asp Phe Leu acc ttc gtg Thr Phe Val Leu aat As n ggo Gly act Th r 250 gat Asp ggt Gly gat Asp ggg Gly g ca Ala Ser too Ser ctg Leu gtg Vali 255 gaa Glu gt t ValI aac As n ct g Leu tac Tyr Gin 210 ctg Leu oct Pro agt Ser Ott Leu got Ala 290 ca 0 His acc Th r gtg ValI Val1 og t Arg ago Se r aa o Asn 900 Ala 275 ga t Asp ca g Gin t go Cys aag Lys PCTA1B00100926 Trp ott 787 Leu caa 835 Gin 245 gaa 883 Glu tgc 931 Cys 000 979 Pro ttt 1027 Ph e gat 1075 Asp 325 atg 1123 Met ggg oto Gly Leu taggtttctt tggaccaaac oao 1152 <210> 318 <211> 343 <212> PRT <213> Corynebacterium giutamicum <400> 318 Val Val Ala Leu Thr Gin Ile Val Gly Pro 1 5 10 Arg Giu Leu Glu Lys Arg Tyr Arg Giu Thr 25 Thr Ala Asp Pro Arg Ala His Ilie Thr Tyr 40 Giu Glu Leu Ala Phe Gly Leu Giu Gin Arg 55 Met Trp Giu Arg Val Arg Asn Ile Gly Leu 70 Ser Gly Ser Gly Leu Pro Giy Ala Val Met Leu Arg Ala Thr Val Gly Ile Val Pro Ala Gly Leu Giu Asn Leu 75 Th r L eu Ala Gin Leu Asp Ile Pro Leu Leu 145 Pro Th r Ser Gly Leu 225 Asn Gly Arg Leu Ile 305 Leu Ile WO 01/00805 Arg Ala Pro Gly Thr Val 100 Leu Ser Gly 115 Glu Ser Tyr 130 Asp Ala Pro Ala Arg Val Gly Thr Arg 180 Gin Pro Gin 195 Gin Val Leu 210 Leu Arg Gly Pro Ser Asp Ser Asn Ser 260 Leu Ala Gin 275 Ala Asp Giu 290 His Gin Arg Thr Cys His Val Lys Glu 340 Gin Ile Asp Gly Val1 150 Ph e Gin Gin Leu Ala 230 Val1 Glu Asp Asp Al a 310 Giu Gly Leu Leu Th r Asp 135 Ty r Ser Gin Ile Gin 215 Asn Ile His Ala Val 295 Asp Thr Leu Ser Glu Ser 120 Val Le u Gly Arg Gly 200 Gly Giu Asp Pro Ala 280 Gly Ph e Ph e Gi y Ala 105 Se r Ile Gly Pro Arg 185 Pro Pro Pro Ser Leu 265 Leu Leu Leu Val1 Gi y Pro Arg Val1 Asp Ser 170 Arg Leu Asn Gly Thr 250 Asp Gly Asp Gly Ala 330 PCTIBOO/00926 Al a Asp Met Trp Leu 160 Ile Giu Ala Th r Gin 240 Pro Leu Leu Ala Ile 320 Al a <210> 319 <211> 1746 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (1723) <223> RXN02074 WO 01/00805 <400> 319 cgggggaagg ccgtgtcgca tgctcgggct agccttggat tttaaagtct gggctttaag tgcagaaagg ttgtggattg PCTIBOO/00926 ct caagaaga attcgactgg cg t Ar g cg C Arq ga c Asp gg t Gly caa Gin gct Al a atc Ile a tg Met att Ile tgc Cys 150 atg Met g ca Ala ctg Leu aat ga t Asp acg Th r gt c Val1 gcc Ala cgt Arg aaa Lys aca Th r ttt Ph e gcc Ala 135 ctc Leu t tg Leu 9gc Gly aat Asn atg atc Ile ctc Leu ccc Pro a cg Thr att Ile aat As n ctg Leu ct g Leu 120 gtt ValI ggg Gly aaa Lys gtg ValI gcc Ala 200 cgc g cg Ala gt t Val ccc Pro gca Al a gca Al a gag Giu act Th r cct Pro ggc Gly tgc Cys gc t Ala 170 ttg Le u gg g Gly ca a gtg Val1 gcg Ala gct Ala qct Ala cqa Arg 75 ctq Leu gaa G iu tac T yr gcg Ala gtg Val1 155 qgc Gly gaa Giu ca g Gin gtg ggt Gly ctg Leu cat His gcg Ala 60 gt t ValI aat Asn gcc Ala ttt Ph e gc t Ala 140 ata Ile gcg Ala t cg Ser cg c Arg atg t gg T rp gtc Val1 t ca Ser 45 ctg Leu gaa Glu tcc Ser acc Th r gca Ala 125 att Ile cct Pro gga Gly gtg Val1 agg Arg 205 tca atc Ile 15 gt t Val1 atg Met t gc Cys tcc Se r tcg Ser 95 aaa Lys ttt Ph e tqg Trp gt t Val ggg Gly 175 aca Thr atc Ile ct g acc Th r gg g Gly ttq Le u gcg Al a tgg Trp 80 t ca Ser gca Aia t tg Leu ccg Pro att Ile 160 cga Arg cta Leu atc Ile tac a tg Met 1 gcg Al a atc Ile t gg Trp gaa Glu c9g Arg ga t Asp t cc Ser gcc Al a att Ile 145 t ct Ser gca Ala ggc Gly aca Th r cga a cg Thr ggc Gl y t gg Trp gcg Al a cgg Arg ga t Asp act Thr cca Pro 130 gcg Ala tgg T rp tct Ser acc Thr ca g Gin 210 aat gt t Val1 ctt Leu ct g L eu ctc Leu t tg Leu ca g Gin 100 aca Th r aca Thr ata Ile ca g Gin cag Gin 180 atg Met gca Ala ttg gt g Val1 atc Ile gca Al a ccc Pro gct Ala ttg Le u gt g ValI gtt Val1 ctg Leu cg c Arg 165 t tg Leu atg Met gag Giu at g cgc tcc ctg ctt Arg Ser Leu Leu l's 163 211 259 307 355 403 451 499 547 595 643 691 739 787 WO 01/00805 WO 0100805PCTA~BOO/00926 Asn Met Arg Ser Gin Val Met Ser Leu Leu att Ile 230 gcg Al a caa Gin aac As n ct g Leu act Thr 310 aag Ly s att Ile aaa Lys gcg Ala tca Ser 390 aaa Lys cac His ga a Giu 215 ctg Leu g tg Val qct Aia cgc Arg atc Ile 295 caa Gin ato Ile ccc Pro t cc Ser att Ile 375 gtc Val agc Se r gca Al a acc Thr ga c Asp att Ile 250 ct C Leu cgc Arg ga a Glu ca 9 Gin ga t Asp 330 tcc Se r gc t Ala gg c Gly gtg Val gat Asp 410 tta 'Leu ggt Giy gg C Gi y 235 gga Giy gta Val a cq Thr aaa Lys cgc Arg 315 ca c His cac His cta Leu ggc Giy Pro 395 ctg Leu ggc Gly gtC Val ttt Ph e ttc Phe ctg Leu tac Tyr 285 ctc Leu ga t Asp gac Asp g cg Ala t tg Leu 365 aac As n tcc Ser cgc Arg gaa Glu 99c Giy 445 gga gtt Giy Val ttt tca Phe Ser 255 gcc agg Ala Arg 270 acc ggc Thr Gly gcg aca Ala Thr 999 gat Gly Asp att 9t9 Ile Val 335 gtg gta Val Val 350 tcc gga Ser Gly tgt gag Cys Glu ccc acg Pro Thr acg ggt Thr Gly 415 ctc ccc Leu Pro 430 ggc caa Gly Gin gcc gtg Ala Val Tyr gCC Aia 240 ggc Giy ct g Leu at g Met a cc Thr ct g Leu 320 cac His ggt Gly ctt Leu atg Met ctg Leu 400 gt t Val1 gcg Al a g ca Aia att Ile Arg 225 acc Th r tct Ser ct t Leu gca Ala ttt Phe 305 gt 9 Val1 ggt Gi y ccc Pro tta Leu tta Leu 385 ttt Phe gat Asp ga c Asp gca Al a gt t Val1 Asn aca Th r ctt Leu att Ile Gi y 290 act Th r gt c ValI atc Ile agt Se r gag Glu 370 ga t Asp agc Ser tct Ser ctc Leu cgc Arg 450 ctc Le u Met gct Ala 245 ggc Gly atc Ilie t cg Ser cca Pro ttg Leu 325 agc Se r ggt Gly ggt Gi y gcc Aia atc Ile 405 atc Ile ggt Gly att Ile gcg Ala 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 gcc cga ggt Ala Arg Gly tta gta aag aat gct Leu Val Lys Asn Ala WO 01/00805 455 460 acc gca caa ctc gac tac acc aac Thr Ala Gin Leu Asp Tyr Thr Asn 470 475 aaa tcc ctt gag tgc acg ttg gtt Lys Ser Leu Glu Cys Thr Leu Val 490 gcc ctc gat gca gac ttc atc att Ala Leu Asp Ala Asp Phe Ile Ile 505 atg atg gat aca ccc agc aac gtt Met Met Asp Thr Pro Ser Asn Val 520 525 acc gct gtg atg gag gaa gaa caa Thr Ala Val Met Glu Glu Glu Gin 535 540 <210> 320 <211> 541 <212> PRT <213> Corynebacterium giutamicun PCTIBOO/00926 465 gcc cgc cag gtt cga cat ctt Ala Arg Gin Val Arg His Leu 480 gag atc acc cac cgc cca tca Glu Ilie Thr His Arg Pro Ser 495 500 gtt tta gag gat ggc caa ttg Val Leu Glu Asp Gly Gin Leu 510 515 tcc cag cac aat gcg ttt ttc Ser Gin His Asn Ala Phe Phe 530 tgatttcccg acttctccaa ttg 1555 1603 1651 1699 1746 <400> 320 Met Arg Ser Leu Leu Arg Asp Ile Pro 1 Ala Ile Trp Glu Arg Asp Ser Ala Ile 145 Ser Th r Gly Trp Ala Arg Asp Thr Pro 130 Al a Trp L eu Pro Th r Ile Asn Leu Le u 120 Vai Gly Lys ValI Ser Ala Arg Leu Al a 105 Gly ValI Leu Gly Ala 10 ValI Pro Ala Al a Giu Thr Pro Gly Cys Ala 170 Gly Leu His Al a Val As n Ala Phe Ala 140 Ile Ala Trp ValI Ser Leu Glu Ser Thr Al a 125 Ile Pro Gly L eu Ile Ala Leu Arg Se r Ser 110 Val1 Ser Ph e Tyr Ile Val1 Met Cys Ser Ser Lays Phe T rp ValI Gly 175 Th r Gly Le u Ala T rp Ser Ala Le u Pro Ile 160 Arg WO 01/00805 PTIO/02 PCT/IBOO/00926 Ala Ser Gly Thr Thr Gin 210 Arg Asn 225 Thr Thr Ser Leu Leu Ile Ala Gly 290 Phe Thr 305 Val Val Gly Ile Pro Ser Leu Giu 370 Leu Asp 385 Phe Ser Asp Ser Asp Leu Ala Arg 450 Val Leu 465 Val Arg Gly Thr 195 Arg Gin Met Th r Asp 275 Lys Asp As n Ser Giy 355 Phe Leu Gly Asp Lys 435 Ile Asp His Gin 180 Met Ala Leu Val1 Leu 260 Pro Pro Gin Asn Ph e 340 Ala Asp Arg Ser His 420 ValI S er Giu Leu Leu Ala Gly Val Phe Leu Glu Ser Val Arg Thr Leu 185 190 Met Glu Met Ala 245 Gi y Ile Ser Pro Leu 325 Ser Gly Gly Ala Ile 405 Ile Gly Ile Aia Ala 485 Leu As n Ile 230 Al a Gin As n Leu Thr 310 Lys Ile Lys Ala Ser 390 Lys His Glu Ala Thr 470 Lys Giy Gin Asp Ile 250 Leu Arg Glu Gin Asp 330 Ser Al a Gly Val1 Asp 410 Leu Giy Val1 Asp C ys 490 Gin Val1 Gly 235 Gi y Val Th r Lays Arg 315 His His Leu Giy Pro 395 Leu Gly Val1 Lys Tyr 475 Thr Arg Met 220 Vai Giy Leu Ph e Ala 300 His Arq Ile Ala His 380 Gin Ala Giu Ser As n 460 Th r Le u Ile Tyr Ala 240 Gly Leu Met Thr Leu 320 His Gly Leu met Le u 400 Vali Ala Ala Ile Gin 480 Thr WO 01/00805 PCTIIBOOIOO926 His Arg Pro Ser Glu Ala Leu Asp Ala Asp Phe Ile Ile Val Leu Giu 500 505 510 Asp Gly Gin Leu Thr Met Met Asp Thr Pro Ser Asn Val*Ser Gin His 515 520 525 Asn Ala Phe Phe Arg Thr Ala Val Met Glu Giu Glu Gin 530 535 540 <210> 321 <211> 1746 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1723) <223> FRXA02074 <400> 321 cgggggaajg ccgtgtcqca tgctcgggct agccttggat ctcaagaaga attcgactgg tttaaagtct gggctttaag tgcagaaagg ttgtggattg atg cgc tcc ctg ctt 115 Met Arg Ser Leu Leu 1 cgt gat atc cct gcg gtg ggt tgg cta atc acc gcg acg att gtt gtg 163 Arg Asp Ile Pro Ala Val Gly Trp Leu Ile Thr Ala Thr Ile Val Vai 15 cgc acg ctc gtt gtt gcg ctg gtc atc gtt qqq atc ggc ttg ctt atc 211- Arg Thr Leu Val Val Ala Leu Val Ile Val Gly Ile Gly Leu Leu Ile 30 gac gtc ccc tcg ccc gct cat tca gcc atg ttg tgg tgg gtt ctg gca 259 Asp Val Pro Ser Pro Ala His Ser Ala Met Leu Trp Trp Val Leu Ala 45 ggt gcc acg gca gca gct gcg ctg ctg tgc gcg gaa gcg gtg ctc ccc 307 Gly Ala Thr Ala Ala Ala Ala Leu Leu Cys Ala Giu Ala Val Leu Pro 60 caa cgt att cgt gca cga gtt gaa cga tcc tgg cgg cgg cag ttg gct 355 Gin Arg Ile Arg Ala Arg Val Glu Arg Ser Trp Arg Arg Gin Leu Ala 75 80 gct aaa aat ctg gag ctg aat tcc agt tcg tca gat gat gcc cag ttg 403 Ala Lys Asn Leu Glu Leu Asn Ser Ser Ser Ser Asp Asp Ala Gin Leu 95 100 atc aca ctg gca act gaa gcc acc tca aaa gca tcc act tac aca gtg 451 Ile Thr Leu Ala Thr Giu Ala Thr Ser Lys Ala Ser Thr Tyr Thr Val 105 110 115 atg ttt ctg gqg cct tac ttt gca gta ttt ttg gcc cca ctq aca gtt 499 Met Phe Leu Gly Pro Tyr Phe Ala Val Phe Leu Ala Pro Leu Thr Val 120 125 130 att gcc gtt gtc ggc gcg gct att tcc tgg ccg att gcg ggg ata ctg 547 Ile Ala Val Val Gly Ala *Ala Ile Ser Trp Pro Ile Ala Gly Ile Leu WO 01/00805 PCT/IBOO/00926 135 140 145 tgc ctc ggg ttg tgc gtg ata cct ttc gtt att tct tgg gca cag cgc 595 Cys Leu Gly Leu Cys Val Ile Pro Phe Val Ile Ser Trp Ala Gin Arg 150 155 160 165 atg ttq aaa qgc gct ggc gcg gga tac ggg cga gca tct ggg cag ttg 643 Met Leu Lys Giy Ala Gly Ala Gly Tyr Gly Arg Ala Ser Gly Gin Leu 170 175 180 gca ggc gtg ttt ttg gaa tcg gtg cgc aca cta ggc acc acg atg atg 691 Ala Gly Val Phe Leu Giu Ser Val Arg Thr Leu Gly Thr Thr Met Met 185 190 195 ctg aat gcc gct ggg cag cgc agg cag atc atc aca cag cgc gca gag 739 Leu Asn Ala Ala Gly Gin Arg Arg Gin Ile Ile Thr Gin Arg Ala Giu 200 205 210 aat atg cgc tcc caa gtg atg tca ttg ctg tac cga aat cag ttq atg 787 Asn Met Arg Ser Gin Val Met Ser Leu Leu Tyr Arg Asn Gin Leu Met 215 220 225 att ctg gtg acc gac ggc gtg ttt gga gtt gcc acc aca atg gtt gct 835 Ile Leu Val Thr Asp Gly Val Phe Gly Val Ala Thr Thr Met Val Ala 230 235 240 245 gcg gtg ttt gcc att gga gga ttc ttt tca ggc tct ctt act ctc ggc 883 Ala Val Phe Ala Ile Gly Gly Phe Phe Ser Gly Ser Leu Thr Leu Gly 250 255 260 caa gct gta gca ctc gta ttg ctg gcc agg ctg ctt att gat ccc atc 931 Gin Ala Val Ala Leu Val Leu Leu Ala Arg Leu Leu Ile Asp Pro Ile 265 270 275 aac cgc atg ggt cgc acg ttt tac acc ggc atg gca ggc aaa ccc tcg 979 Asn Arg Met Gly Arq Thr Phe Tyr Thr Gly Met Ala Gly Lys Pro Ser 280 285 290 ctg atc gcc att gaa aaa qcc ctc gcq aca acc ttt act gat cag cca 1027 Leu Ile Ala Ile Giu Lys Ala Leu Ala Thr Thr Phe Thr Asp Gin Pro 295 300 305 act caa cag gga cag cgc cac gat ggg gat ctg gtg gtc aac aac ttg 1075 Thr Gin Gin Giy Gin Arg His Asp Gly Asp Leu Vai Vai Asn Asn Leu 310 315 320 325 aag atc gcc cgc gat cac agg gac att gtg cac ggt atc tct ttc agc 1123 Lys Ilie Ala Arq Asp His Arg Asp Ile Val His Gly Ile Ser Phe Ser 330 335 340 att ccc cgc ggt tcc cac atc gcg gtg gta ggt ccc agt ggc gct ggt 1171 Ile Pro Arg Giy Ser His Ile Ala Val Vai Giy Pro Ser Gly Ala Gly 345 350 355 aaa tcc tct gtg gct cta gcg ttg tcc gga ctt tta gag ttt gat ggt 1219 Lys Ser Ser Val Ala Leu Ala Leu Ser Gly Leu Leu Giu Phe Asp Gly 360 365 370 gcg att tcc ctc ggc ggc cac aac tgt gag atg tta gat ctt cgc gcc 1267 Ala Ile Ser Leu Gly Gly His Asn Cys Giu Met Leu Asp Leu Arg Ala 375 380 385 WO 01/00805 WO 0100805PCTIIBOO/00926 tca gtc agt ttc gtq ccc caa tcc Ser Val Ser Phe Val Pro Gin Ser 390 395 aaa agc aat atc gat ctg gcg cgc Lys Ser Asn Ile Asp Leu Ala Arg 410 cac gca gca ctt tta ggc gaa gaa His Ala Ala Leu Leu Gly Giu Glu 425 gaa acc ggc aaa ggt gtc tcc qgc Giu Thr Gly Lys Gly Vai Ser Gly 440 445 gCC cga ggt tta gta aag aat gct Ala Arg Gly Leu Vai Lys Asn Ala 455 .460 acc gca caa ctc gac tac acc aac Thr Ala Gin Leu Asp Tyr Thr Asn 470 475 aaa tcc ctt gag tgc acg ttg gtt Lys Ser Leu Giu Cys Thr Leu Val 490 gcc ctc gat gca gac ttc atc att Ala Leu Asp Ala Asp Phe Ile Ile 505 atg atg gat aca ccc agc aac gtt Met Met Asp Thr Pro Ser Asn Val 520 525 acc gct gtg atg gag gaa gaa caa Thr Ala Vai Met Glu Glu Giu Gin 535 540 <210> 322 <211> 541 <212> PRT <213> Corynebacteriun glutamicun <400> 322 Met Arg Ser Leu Leu Arg Asp Ile 1 5 Ala Thr Ile Val Val Arg Thr Leu Ile Giy Leu Leu Ile Asp Val Pro 40 Trp Trp Vai Leu Ala Giy Ala Thr 55 Giu Ala Val Leu Pro Gin Arg Ile ccc acg ctg ttt agc Pro Thr Leu Phe Ser agc atc Ser Ile a cg Thr ctc Leu 430 ggc Gly gcc Ala gcc Ala gag Giu gt t Val1 510 tcc gg t Gly 415 ccc Pro caa Gin gtg Val1 cgc Arg atc Ile 495 tta Leu cag 400 gt t Val gcg Ala gca Al a att Ile ca g Gin 480 a cc Thr gag Giu cac ga t Asp gac Asp g ca Ala gtt Val 465 gt Vai cac His ga t Asp aat t ct Ser ctc Leu cgc Arg 450 ct C Le u cga Arg cqc Arg ggC Gly gcq 1315 1363 1411 1459 1507 1555 1603 1651 1699 1746 Ser Gin His Asn Ala Phe Phe Arg 530 tgatttcccg acttctccaa ttg Val Giy Trp Leu Ile Thr Ala Leu Val Ile Val Giy Ala His Ser Ala Met Leu Ala Ala Leu Leu Cys Ala Arg Vai Giu Arg Ser Trp WO 01/00805 WO 0100805PCT/iBOO/00926 Arg Asp Ser Ala Ile 145 Ser Ala Gly Thr Arg 225 Thr Ser Leu Ala Phe 305 Val1 Gly Pro Leu Leu 385 Arg Asp Th r Pro 130 Ala Trp Se r Th r Gin 210 As n Th r Leu Ile Gi y 290 Thr Val Ile Ser Giu 370 Asp Gin Ala Tyr 115 Leu Gly Ala Gly Thr 195 Arg Gin Met Thr Asp 275 Lys Asp Asn Ser Gly 355 Phe Le u Leu Gin 100 Th r Thr Ile Gin Gin 180 Met Ala Leu ValI Leu 260 Pro Pro Gin Asn Phe 340 Ala Asp Arg Al a Leu Val Val Leu Arg 165 Leu Met Giu Met Ala 245 Gly Ile Ser Pro Leu 325 Ser Gly Gly Ala Al a Ile Met Ile Cys 150 Met Ala Leu As n Ile 230 Ala Gin As n Leu Th r 310 Lys Ile Lys Al a Ser 390 Lys Th r Phe Ala 135 Leu Leu Gly As n Met 215' Leu ValI Ala Arg Ile 295 Gin Ile Pro Ser Ile 375 Val1 Asn Leu Leu 120 Val1 Gly Ly s Val1 Al a 200 Arg Val Phe ValI Met 280 Ala Gin Ala Arg S er 360 S er S er Leu Al a 105 Gly ValI Leu Gly Phe 185 Ala Ser Th r Ala Al a 265 Gly Ile Gly Arg Gly 345 Val Leu Ph e Giu 90 Th r Pro Gi y Cys Al a 170 Leu Gi y Gin Asp Ile 250 Le u Ar q Giu Gin Asp 330 Ser Ala Gly Val1 Leu Glu T yr Ala Val1 155 Gly Glu Gin Val1 Gly 235 Gly Val Th r L ys Arg 315 His His Leu Gly Pro 395 As n Al a Phe Al a 140 Ile Al a Ser Arg Met 220 Val1 Gly Leu Phe Ala 300 His Arg Ile Ala His 380 Gin S er Th r Ala 125 Ile Pro Gly Val Arq 205 S er Ph e Ph e Le u Tyr 285 Leu Asp Asp Ala Leu 365 Asn Ser Ser Ser 110 Val1 Ser Ph e Tyr Arg 190 Gin Le u Gly Phe Ala 270 Thr Ala Gly Ile Val 350 Ser Cys Pro S er Lys Phe Trp ValI Gly 175 Thr Ile Leu Val1 Ser 255 Arg Gly Th r Asp Val1 335 Val1 Gly Glu Th r Ser Al a Leu Pro Ile 160 Arq Leu Ile Tyr Ala 240 Gly Leu Met Th r Leu 320 His Gly Leu Met Leu 400 WO 01/00805 Phe Ser Gly Ser Ilie Lys Ser Asn Ile Asp Leu 405 410 Asp Ser Asp His Ilie His Ala Ala Leu Leu Gly 420 425 Asp Leu Lys Val Gly Glu Thr Gly Lys Gly Val 435 440 Ala Arg Ile Ser Ilie Ala Arg Gly Leu Val Lys 450 455 Val Leu Asp Glu Ala Thr Ala Gin Leu Asp Tyr 465 470 475 Val Arg His Leu Ala Lys Ser Leu Glu Cys Thr 485 490 His Arg Pro Ser Giu Ala Leu Asp Ala Asp Phe 500 505 Asp Gly Gin Leu Thr Met Met Asp Thr Pro Ser 515 520 Asn Ala Phe Phe Arg Thr Ala Val Met Glu Giu 530 535 <210> 323 <211> 1527 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1504) <223> RXA02095 <400> 323 ctctcttggt cctctcccca cccattttta agtactcaag tactccccca acaggctcaa aaatactgaa agqctcacgc PCTIIBOO/00926 Thr Leu 430 Gl y Ala Ala Glu Val1 510 Ser Gly 415 Pro Gin Val Arg Ile 495 Le u Gin Val Ala Ala Ile Gin 480 Thr Giu His tcc Ser cgc Arg tca Se r ctt Leu caa Gin atc Ile ttc Phe cct Pro tta Le u att Ile agc Ser ggt Gly gCC Ala tcc Ser gct Ala ggc Gly 60 CCt Pro gtg Val tta Leu gcc Ala aag Lys gcg Ala qct Ala a tc Ile acccttccaa cagaaaggat atg aaa act gag caa 115 Met Lys Thr Giu Gin 1 cct gaa aag cca caa 163 Pro Giu Lys Pro Gin cga cct tgg ctc acc 211 Arg Pro Trp Leu Thr ttg ttt gaa ctt aca 259 Leu Phe Giu Leu Thr gcg ctc gga aat acc 307 Ala Leu Gly Asn Thr WO 01/00805 WO 0100805PCT/iBOOI0O926 gga Gly tta Leu ctt Le u agc Ser cag Gin qt c Val1 150 atg Met gtg Val1 t tg Leu ttt Phe cat His 230 cag Gin ttt Phe a tg Met qgt Gly gat Asp agc S er cgc Arg a tg Met aac As n 135 agt S er ttg Leu atc Ile gtg Val1 gcg Ala 215 gtg Vali ga a Giu gCC Aia gt t Val act Th r gtg Vai tat Tyr ggg Giy 120 ctc Leu c9g Arg ccg Pro atq Met cct Pro 200 t cc Ser gaa Giu gac Asp cag Gin gag Giu 280 tta acc act gac ctg ctg gac cgg ttc Leu Thr Thr Asp Leu Leu Asp Arg Phe 75 80 ttg acc agc gtc att gcc ctt atc gtg Leu Thr Ser Vai Ile Ala Leu Ile Val gcc agt caa ttt gga cgg cga tac acc Ala Ser Gin Phe Giy Arg Arg Tyr Thr 105 110 gta cag cat gat gtc cgg ctt aaa acg Vai Gin His Asp Vai Arg Leu Lys Thr 125 gat ggg cca ggt cag gac tct ait cgc Asp Gly Pro Gly Gin Asp Ser Ile Arg 140 145 tcc att tcg gat atc aac atg gtg caa Ser Ile Ser Asp Ile Asn Met Vai Gin 155 160 atg ttg atc gga aat gtg gtc aag ctt Met Leu Ile Gly Asn Vai Vai Lys Leu 170 175 ctq gct att tcc ccg ccg ctg acc atc Leu Aia Ile Ser Pro Pro Leu Thr Ile 185 190 ttg ctg ttg tgg gcc gtg gcc tat tcg Leu Leu Leu Trp Ala Val Ala Tyr Ser 205 acg tgg tcg gcc cag caa aag gct gcg Thr Trp Ser Ala Gin Gin Lys Ala Ala 220 225 gaa act gtc acg ggt atc cgc gtg gtc Giu Thr Vai Thr Giy Ile Arg Val Val 235 240 cgc gag acc gac aaa ttg gat ctc acc Arg Giu Thr Asp Lys Leu Asp Leu Thr 250 255 cgc atg cgc act gca cgt ctg acg gca Arg Met Arg Thr Aia Arg Leu Thr Aia 265 270 cag ctt ccg cag ctt gct ttg gtg gtc Gin Leu Pro Gin Leu Ala Leu Val Val 285 act ccg agt gga Thr Pro Ser Gly t tg Leu ctc Leu t tg Leu gta Vali gcg Ala 165 ttg Leu gtg Vali ctt L eu act Thr g cg Ala 245 tta Leu ccc Pro ggc Gly 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 ggc tat ttg gcc atg act Gly Tyr Leu Ala Met Thr 295 300 cac atc acg gtg ggc acg ttt gtg His Ile Thr Val Gly Thr Phe Val 305 WO 01/00805 gcg ttt tct tcc tat ctc act agc ttg tcg PCTIBOOIOO926 gcg gtg gct Ala Val Ala agg tcc ctg Arg Ser Leu Ala 310 t cg Ser atc Ile ccc Pro gat Asp g aa Glu 390 gtg Val ttt Phe gat Asp tcc Phe ggc Gly ttt Phe ctg Leu ttc Ph e 375 acc Th r ca q Gin ga t Asp atc Ilie t cc Ser atg Met gaa Giu t ca Ser 360 cg t Arg gt t Vali ct t Leu agc Ser cgc Arg 440 tcc Ser Tyr Leu 315 ctc atg cgc Leu Met Arg 330 *gtc att gat Val Ile Asp 345 ctt ccc gac Leu Pro Asp ggg att ctc Giy Ile Leu gtg ttg gtg Vai Leu Val 395 gct gga aac Ala Giy Asn 410 aac ggc cat Asn Giy His 425 agg aat ctc Arg Asn Leu ata ccg cga Thr Ser Leu Ser 320 325 t tg Leu cct Pro 350 ctg Leu ttt Ph e cca Pro caa Gin cgc Arq 430 qt t Vali t ct Ser acc Th r t cg Ser qg t Giy 385 ggc Gly agc Ser gac Asp qag Glu t ct Ser ga t Asp ttc Phe 370 gt t Val aa 9 Lys ggc Gly ctc Leu ccg Pro 450 Val1 cct Pro 355 aac Asn ca g Gin acc Th r cac His acc Th r 435 ttc Ph e cg C Arg cac His gta Val 99 t Gly gc t Ala 405 gcc Aila agc Ser tac Tyr 1075 1123 1171 1219 1267 1315 1363 1411 1459 1504 1527 Ser Ser Ser Ile Pro Arg Giu 455 460 cat ctc gat ggg ttt gga tgt cag His Leu Asp Giy Phe Giy Cys Gin 465 tgatgagcag atcgaacacg cag <210> 324 <211> 468 <212> PRT <213> Corynebacterium giutarnicum <400> 324 Met Lys Thr Glu Gin Ser Gin Lys Ala Gin Leu Aia Pro Lys Lys Ala 1 5 10 Pro Giu Lys Pro Gin Arg Ilie Arg Gin Le u Ilie Ser Val Ala Trp Gin 25 Arg Pro Trp Leu Thr Ser Phe Thr Vai Ilie Ser Ala Leu Aia Aia Thr 40 Leu Phe Giu Leu Thr Leu Pro Leu Leu Thr Gly Giy Ala Ilie Asp Ile 55 WO 01/00805 Ala Leu Gly Asn Thr Ph e Val1 Th r Th r Arg 145 Gin Leu Ile Ser Ala 225 Val1 Th r Ala Val1 Val1 305 ValI Ser Thr Ser Thr L eu Ala Met 130 Th r Ser Val1 Ile Arg 210 Asp Lys Al a Lys As n 290 Gi y Al a Ser Asp Phe 370 Pro L eu Gly 115 Arg Gly Leu Leu Ala 195 Lys Leu Ala Arg Phe 275 Ile Thr Arg ValI Pro 355 Asn Se r Ala 100 Lys Ser Gin ValI Th r 180 Al a Ala Thr Ph e Glu 260 Ile Val Ph e S er Glu 340 Ala As n Gly Leu Leu Leu Val1 Ala 165 Leu Val1 Leu Th r Ala 245 Leu Pro Gly Val1 Leu 325 Arg His Val1 Gly 70 Leu Leu Se r Gin Val1 150 Met Vali Leu Ph e His 230 Gin Phe Met Gly Ala 310 Ser Ile Pro Asp Ser Arq Met Asn 135 Ser Leu Ile Val1 Al a 215 ValI Glu Ala Val Gly 295 Ph e Gly Phe Leu Phe 375 Asp Thr Leu Thr Thr Asp Leu Leu Asp Val1 Tyr Gly 120 Leu Arg Pro Met Pro 200 Se r Giu Asp Gin Glu 280 Ty r Ser Met Giu Ser 360 Arg Ala Arg 110 Arcj Asp Asn Val Pro 190 Val1 Gin Ile Le u Arg 270 Ala His Leu Leu Pro 350 Leu Phe Pro L eu Arg Leu Ser Met Val1 175 Leu Ala Lys Arg Asp 255 Leu Leu Ile Ser Al a 335 Giu Gly Giu Gly PCTIIBOO/00926 Arg Ile Tyr Lys Ile Val 160 Lys Th r Tyr Ala Val 240 Leu Th r Val Th r Al a 320 Le u Arg Leu Le u Ser Gly Val Gin Ala Gly Giu Thr Val Val WO 01/00805 385 390 395 Gly Lys Thr Met Ala Val Gin Leu Ala Gly Asn Phe Tyr Gin Pro 405 410 415 Ser Giy His Ile Ala Phe Asp Ser Asn Gly His Arg Thr Arg Phe 420 425 430 Asp Leu Thr His Ser Asp Ile Arg Arg Asn Leu Ile Ala Val Phe 435 440 445 Giu Pro Phe Leu Tyr Ser Ser Ser Ile Pro Arq Giu His Leu Asp 450 455 460 Phe Gly Cys Gin 465 <210> 325 <211> 905 <212> DNA <213> Corynebacterium giutamicum PCT/1B00/00926 400 Asp Asp Asp Gi y <220> <221> CDS <222> (882) <223> RXA02225 <400> 325 caa act gag gag Gin Thr Giu Giu 1 atg ttg aag gaa Met Leu Lys Giu ctt gtc cca gcg Leu Vai Pro Ala ttg gct tit gac Leu Ala Phe Asp ttg ctg gtg cca Leu Leu Vai Pro atg aca gag att Met Thr Giu Ile aac tta gtc act Asn Leu Val Thr 100 cgc ttt ggc gca gcg gct gat gaa Arg Phe Giy Ala Ala Aia Asp Giu 5 gct cgt ctg cag tcg ttg ctg act Ala Arg Leu Gin Ser Leu Leu Thr gtg ttt tct gtg ggt ctt ttg gct- Vai Phe Ser Val Gly Leu Leu Ala ggt gac ata act ggt ggt gag atg Gly Asp Ile Thr Gly Giy Glu Met 55 cct tcg ttg act gtg ttg ggt gtg Pro Ser Leu Thr Val Leu Gly Val 70 75 tgg gct agg gga cag gct tcg aca Trp Ala Arg Giy Gin Ala Ser Thr qaa ctg gat aag gcg gcc gct gag Giu Leu Asp Lys Ala Ala Ala Giu 105 gcc ttg gca Ala Leu Ala caa Gin ctg Le u acg Th r atg Met caa Gin cag Gin 96 144 192 240 288 336 cct gcc Pro Ala acc ttt gaa ttt gaa gag ggg atc acg gtg tgg gat cct tcg Thr Phe Giu Phe Glu Giu Gly Ile Thr Val As Pr Se Asp Pro Ser WO 01/00805 WO 0100805PCTIIBOO/00926 aca cct gag gca cgc gat gtg att gat Thr Pro Giu Ala Arg Asp Val Ile Asp 130 135 gtt cgc gaa gat gtc att gtg gct cct Val Arg Glu Asp Val Ilie Val Ala Pro 145 150 ggt gtg ctg aag gat aat tig aat ccg Gly Val Leu Lys Asp Asn Leu Asn Pro 165 atg ctg cgc gct gct ctt cat qct gca Met Leu Arg Ala Ala Leu His Ala Ala 180 185 cga ttg ggt gct gat ctg aac atg ccg Arg Leu Gly Ala Asp Leu Asn Met Pro 195 200 acc ttg atc ggc gag gcc gqa ttg aat Thr Leu Ile Gly Glu Ala Gly Leu Asn 210 215 agg att gct ttg gca cga ttc ttggct Arg Ile Ala Leu Ala Arg Phe Leu Ala 225 230 ttg gat gaa ccg acc acg ggg ttg gat Leu Asp Glu Pro Thr Thr Gly Leu Asp 245 gca cat cgc gtc gaa aag ctt cgt cga Ala His Arg Vai Giu Lys Leu Arg Arq 260 265 acg tcg aac ccg acg tqg cac ggc gtc Thr Ser Asn Pro Thr Trp His Gly Val 275 280 ttt tcg gaa ggg qtg aag tagatggcgc a Phe Ser Giu Gly Val Lys 290 <1>326 <211> 294 <212> PRT <213> Corynebacterium giutamicum <400> 326 Gin Thr Giu Giu Arg Phe Gly Ala Ala 1 5 Met Leu Lys Glu Ala Arg Leu Gin Ser 25 Leu Val Pro Ala Val Phe Ser Val Gly 40 Leu Ala Phe Asp Gly Asp Ile Thr Gly cgg gag Arg Glu cac cgc His Arg 155 atg ggc Met Gly 170 agt tgt Ser Cys ggg gag Gly Giu ctc tcc Leu Ser gtt qat Val Asp 235 gcg gtg Ala Val 250 ggc cgg Gly Arg gca aaq Ala Lys ttg gag Leu Giu 140 gtc agc Val Ser act atc Thr Ile gag gac Glu Asp ttt gag Phe Glu 205 ggt ggc Gly Gly 220 cct gag Pro Giu acc ctg Thr Leu aaa acc Lys Thr cag atg Gin Met gcg ctt cag Ala Leu Gin gtg ttt gaa Val Phe Giu 160 gca ccg gag Ala Pro Giu 175 atc ttg agc Ile Leu Ser 190 ctt cca gat Leu Pro Asp caa cgc cag Gin Arg Gin gtg ctc att Val Leu Ile 240 gat gaa gtg Asp Glu Val 255 gtc gtc att Val Val Ile 270 caa tct qat Gin Ser Asp 432 480 528 576 624 672 720 768 816 864 igcatgagcg cgt Ala Asp Giu Ala Leu Ala Ile 10 Leu Leu Thr Phe Val Arg Gin Leu Leu Ala Tyr Ala Ser Leu Gly Giu Met Ile Ser Val Thr WO 01/00805 WO 0100805PCTIBOO/00926 Le u Met Asn Pro Th r Val1 145 Gly Met Arq Th r Arg 225 Leu Ala Th r Phe Leu Thr Le u Al a Pro 130 Arg Val Leu Leu Leu 210 Ilie Asp His Ser Ser 290 Pro Ile Thr 100 Ph e Ala Asp Lys Al a 180 Al a Gly Leu Pro Val1 260 Pro Pro Trp Giu Glu Arg Val Asp 165 Ala Asp Glu Ala Th r 245 Glu Th r Ser Leu Thr 70 Ala Arg Gly Leu Asp Lys Phe Giu Glu 120 Asp Val Ile 135 Ile Val Ala 150 Asn Leu Asn Leu His Ala Leu Asn Met 200 Ala Gly Leu 215 Arg Phe Leu 230 Thr Gly Leu Lys Leu Arg Trp His Gly 280 Val1 Gl n Ala 105 Gly Asp Pro Pro Ala 185 Pro Asn Al a Asp Arg 265 Leu Ala 90 Ala Ile Ar q His Met 170 Ser Gly Leu Val Al a 250 Gly Gly 75 S er Ala Th r Glu Arg 155 Gly Cys Glu Ser Asp 235 Val1 Arg Se r Lys Pro T rp 125 Glu Ser Ile Asp Giu 205 Gly Giu Leu Thr Leu Arg Arg 110 Asp Ala Val1 Ala Ile 190 Leu Gin Val Asp ValI 270 Gly Val1 Pro Pro Leu Phe Pro 175 Leu Pro Arg Leu Glu 255 Val1 Met Gin Gin Ser Gin Giu 160 Glu Ser Asp Gin Ile 240 Val Ile Val Ala Lys Gin Met Gin Ser Asp Gly Val Lys <210> 327 <211> 1050 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1027) <223> RXA02253 <400> 327 tgaggacatt tcccaagtga aaggcttcgg cccgaaactt gcggaggctg tctatgaagg WO 01/00805 tcttcacgcg tcaaaataag tagatcgcta ggatgtaac atg att caa tcc Met Ile Gin Ser PCTIBOOIOO926 act 115 Thr ggg Gly agg Arg Ct g Leu ca c His cgg Arg tcc Ser ga t Asp gtg Vali ggC Giy t ct Ser 150 t CC Ser gCC Aia Giy a ac Asn cca qtC Val1 ga C Asp a gc Ser aat Asn gaa Giu c9 t Arg aag Lys ctg Leu agc Ser 135 cct Pro gtg Vai acg Thr tca Se r cca Pro 215 gta a cg Thr aat As n aca Th r att Ile gat Asp gaa Giu cag Gin atc Ile 120 caa Gin qtg Vai cat His cgc Arg cca Pro 200 ttc Phe t ct cac His ttc Phe g ca Al a cca Pro tct Ser ttc Phe ctc Leu 105 aag Lys acc Th r aa 9 Lys ga t Asp acc Th r 185 C9 C Arg tgg Trp gac act Thr aca Thr q cc Ala C ca Pro

CCC

Pro cgc Ar g gat Asp cga Arg ctt Leu ga a G iu t t9 Leu 170 cag Gin ga C Asp gtt Vali tat gat Asp cCt Pro cga Arg cag Gin gtc Vai 75 gga Giy

CCC

Pro ttc Phe cag Gin gac Asp 155 cgc Arg cac His 9CC Aia cca Pro 9 t 9 aag Ly s gt c Vai gtc Vali atc Ile 60 ga C Asp agc Ser a cg Thr ga t Asp gt 9 Val1 140 gct Aila CgC Arg gt C Vali ga C Asp gag Glu 220 Ct C tct Ser atc Ile ctc Leu 45 atc Ile aaa Lys ctC Leu gt 9 Vali aa t As n 125 g9t Giy tca Ser 9CC Ala acc Th r ttt Phe 205 ctg Leu t CC gca Ala atc Ile 30 gaa Giu ctg Le u gt t Vali acc Thr tta Leu 110 gtg Vali att Ile gtg Val1 atc Ile att Ile 190 gt t Val1 Cg C Arg caa caa Gin 15 acc Th r ga C Asp gaa Giu gca Aia ca 9 Gin 95 ttt Phe CqC Arg gaa Giu gtc Val ga a Giu 175 ga a Giu gtg Vali cca Pro aaa ga a Glu 9t Giy ttq Leu tta Leu gtc Val1 80 gtt Val1 ct9 Leu Cg C Arg cgt Arg atc Ile 160 t CC Ser tca Ser gac Asp ttt Phe 9 9C aat Asn a tg Met ggt Gly att Ile gtg Val1 gt t Vali gaa Giu acc Thr ga a Giu 145 ga t Asp t Cg Ser ttc Ph e gtg Val1 agg Arg 225 gca cct Pro t ca Ser tgg Trp gat Asp tgc C ys tca Ser 9 ca Ala cat His 130 cga Arg acc Thr ttt Phe 99 t Giy cg t Arg 210 gga Giy gaa gtg Val 99c Gly tat Tyr atg Met ga t Asp gag Giu Cgc Arg 115 cct Pro acc Thr tcg Ser agg Arg ttc Phe 195 ttc Phe gt 9 Vali qaa aa 9 Lys g ca Ala gtg Val1 tgc Cys gtg Val Ct 9 Leu 100 gat Asp ttg Leu gtg Val1 ga t Asp a ca Thr 180 aaa Lys t t9 Leu gac Asp ttt t ac T yr 99t Giy gcg Aila 9ct Aia Cg C Arg C9 t Arq gag Giu cag Gin Ctq Leu Ct 9 Leu 165 atc Ile cac His CCg Pro a ag L ys ttg 163 211 259 307 355 403 451 499 547 595 643 691 739 787 835 WO 01/00805 Pro Val Ser Asp Tyr Vai Leu Ser Gin 230 235 aac aac ttt gtg qac atq ctc aaa gac Asn Asn Phe Val Asp Met Leu Lys Asp 250 gaa gga aaa aac ttc atc aca atc ggt Glu Gly Lys Asn Phe Ile Thr Ile Gly 265 270 cac aga tca gtt gcg gtg tct qaa gaa His Arg Ser Val Ala Val Ser Giu Glu 280 285 cag acc acg ctc gac gtg tct gta qta Gin Thr Thr Leu Asp Val Ser Val Val 295 300 taggaaaggg gccaactaat tga <210> 328 <211> 309 <212> PRT <213> Corynebacterium glutamicum <400> 328 Met Ile Gin Ser Thr Gly Val Thr His Giu gga Giy a cc Thr aga Arg 290 att Ile Glu tac Tyr gg t Giy 275 atc Ile aac As n PCTIBOO/00926 Leu 245 cac 883 His cac 931 His gat 979 Asp cac 1027 His 1050 1 Asn Met G ly Ile Val Val Giu Th r Giu 145 Asp Arg Leu His Arg Ser Asp Val Gly Ser 150 Ser Asp Se r As n Giu Arg Lys Le u Ser 135 Pro Val1 Ala Ile Glu Leu Val Th r L eu 110 Val Ile Val1 Ile Gin Thr Asp Giu Ala Gin Phe Ar g Gi u Val1 Glu 175 WO 01/00805 Ser Phe Arg Thr Ile Ala Thr Arg Thr Gin His 180 185 Phe Giy Phe Lys His Giy Ser Pro Arg Asp Ala 195 200 Val Arg Phe Leu Pro Asn Pro Phe Trp Val Pro 210 215 Arg Gly Val Asp Lys Pro Val Ser Asp Tyr Val 225 230 235 Ala Giu Giu Phe Leu Asn Asn Phe Val Asp Met 245 250 Pro Gly Tyr Arg His Glu Gly Lys Asn Phe Ile 260 265 Cys Thr Gly Gly His His Arg Ser Val Ala Val 275 280 Lys Arg Ile Ala Asp Gln Thr Thr Leu Asp Val 290 295 Asp Ile Asn Arg His 305 <210> 329 '<211> 558 <212> DNA <213> Corynebacterium qiutamicum <220> <221> CDS <222> (101)..(535) <223> RXN01881 <400> 329 accggccctg cggcctcaac cgccgaccag cgcgqcqcac taaagacaaa cttaagtatc ggagtcgaag aaaaaccaca PCTIIBOO/00926 Ser Asp Phe Gly 240 Leu Gly Ala Arg aat Asn g t Gi y ctc Leu gaa Glu gag Giu t cc S er ggt Gly ccg Pro aaa Lys ca a Gin acc Thr cg t Arg tgg Trp ggC Gly ct g Leu tct Se r acattttgac tgtttcataa atg gcc aat ctg att Met Ala Asn Leu Ile 1 aaa acg ctt ctc qac Lys Thr Leu Leu Asp att ggc gtc gtc ggc Ile Gly Val Val Gly gta ctt act ggc atc Val Leu Thr Gly Ile tct gac ctg cgc atg Ser Asp Leu Arg Met 115 163 211 259 307 WO 01/00805 gct gtg gtg aeg cag cgt gct gaa ctc Ala Val Val Thr Gin Arg Ala Glu Leu 75 gac gtg gtq ctt gga cct ttg ggt ttg Asp Val Val Leu Giy Pro Leu Gly Leu aac qcc acg gtq cqc gac gtc ctc ggt Asn Ala Thr Val Arg Asp Vai Leu Gly 105 110 ggc ctt gac acc aag gtg ggc aaa cct Giy Leu Asp Thr Lys Vai Gly Lys Pro 120 125 acg cac caa cct ggc cgc cgc gct gqt Thr His Gin Pro Giy Arg Arg Ala Giy 135 140 gtgctcgacg agc <210> 330 <211> 145 <212> PRT <213> Corynebacterium glutamicum <400> 330 Met Ala Asn Leu Ile Asn Leu Giu Asn 1 5 Lys Thr Leu Leu Asp Gly Val Ser Leu 25 Ile Gly Val Val Gly Leu Asn Gly Gly 40 Val Leu Thr Gly Ile Glu Lys Pro Asp 55 Ser Asp Leu Arg Met Ala Val Val Thr 70 Asp Asp Thr Val Ala Asp Val.Val Leu Phe Glu Trp Aia Ser Asn Ala Thr Vai 100 105 Gly Ile Val Asp Leu Gly Leu Asp Thr 115 120 Val Gly Giu Ala Pro Thr His Gin Pro 130 135 Pro 145 <210> 331 aat As n gaa Glu 95 gqC Gly ttt Ph e t cg Ser ga t Asp gtt Val ttg Le u ccg Pro cga Arg gac Asp ttc P he qgc Gly gtg ValI cct Pro 145 gac acc gtc Asp Thr Val gaa tgggca Giu Trp Ala 100 att gtc gat Ile Val Asp 115 ggc gaa gcg Gly Giu Ala 130 tgacctgatc PCT/IBOO/00926 355 403 451 499 ValI 10 Gly Gly Gin Gin Gly 90 Arg Lys Gly Ser Lys Val Gin Lys Thr Gly Arq Arg Ala 75 Pro Leu Asp Val Val'Gly Arg Arg 140 Th r Thr Thr Val Glu Gly Leu Lys 125 Ala Trp Gly Leu Ser Leu Leu Gly 110 Pro Gi y Gly Asp Leu His As n Glu Gly Phe S er L eu Arg Giu Asn Asp Val1 Leu Pro Arg WO 01/00805 <211> 564 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (541) <223> FRXA01881 <400> 331 acogcctg cggcctcaac cgccgaccag cgcggcgcac PCTAIBOO/00926 acattttgac tgtttcataa taaagaoaaa cttaagtatc ggagtcgaag aaaaaccaoa atg goc aat ctg att Met Ala Asn Leu Ile aat Asn gg t Gly ctc Le u ga a Glu got Al a gao Asp ,aac As n ggo Gly gao gag Glu too Ser ggt Gly ccg Pro gtg Val gtg Val a og Th r gac Asp 120 cca aac Asn tta Leu ggc Gly ga t Asp a cg Thr ott Leu gtg Val1 105 aco Th r ac gt o Val1 ggt Gly gga Gly ca g Gin cag Gin gga Gly cg c Arg aag Lys t gg too Ser gt t Val aaa Lys ggo Gly cgt Arg 75 ct Pro gao Asp gtg Val cg aaa L ys caa Gin acc Thr cgt Arg got Ala ttg Le u gtc Val ggg Gi y cog acc Th r aco Th r aco Thr 45 gtg Val1 gaa Glu ggt Gly ctc Leu caa Gln 125 ogo Arg tgg Trp ggo Gly 30 otg Le u tct Ser ctc Leu t tg Leu ggt Gly 110 aco Th r tgg Trp gga Gi y 15 gao Asp otg Le u ca o His aat Asn gaa Glu 95 ggo Gly ttt Ph e tto Phe tta Leu ogo Arg gaa Glu aao Asn gat Asp 80 gt t Vali ttg Leu too Ser gog Ala 1 aaa Lys att Ile qta Val tot Ser gao Asp tto Phe ggo Gly ggt Gly aco Thr 145 aog- Thr ggc Gly ott Leu gao Asp gao Asp gaa Glu att Ile ggg Gly 130 ttg Leu ott Leu gto Val1 act Thr ctg Leu aco Thr t gg Trp gt o ValI 115 oga Arg aoo Th r oto Leu gto Vali Gly ogo Arg gto Val g oa Ala 100 ga t Asp ago Ser gao Asp ggo Gly ato Ile atg Met got Al a toa Ser ott Le u goo Ala Asp Ala Pro Thr Trp Pro Pro 135 140 tgatogtgot ogaogagooo aoo <210> 332 <211> 147 <212> PRT <213> Corynebacteriun glutamioum <400> 332 WO 01/00805 Met Ala Asn Leu Ile Asn Leu Giu Asn Val Ser 1 5 10 Lys Thr Leu Leu Asp Gly Val Ser Leu Gly Val 25 Ilie Gly Val Val Giy Leu Asn Gly Gly Gly Lys Vai Leu Thr Gly Ile Giu Lys Pro Asp Gin Gly Ser Asp Leu Arg Met Ala Val Vai Thr Gin Arg 70 75 Asp Asp Thr Val Ala Asp Val Val Leu Gly Pro 90 Phe Giu Trp Aia Ser Asn Aia Thr Vai Arg Asp 100 105 Giy Ile Vai Asp Leu Gly Leu Asp Thr Lys Vai 115 120 Giy Giy Arg Ser Aia Asp Ala Pro Thr Trp Pro 130 135 Thr Leu Thr 145.

<210> 333 <211> 813 <212> DNA <213> Corynebacterium giutamicum <220> <22i> CDS <222> (101)..(790) <223> RXA00526 <400> 333 ggtggagcag gcggcggctc cttttagtcc tgcggcccct cgtttctgcc aagcaaaccg tgggccaggt gatttagcct Trp Gly Leu Ser Leu Leu Giy 110 Th r Trp Gly Asp Leu His As n Giu Giy Ph e Ph e PCT/EB00100926 Leu Arg Glu As n Asp Val1 Leu Ser Ala atg Met a cc Thr t cg S er att att Ile cac His qg t Gly ttg gtc Val1 ggt Gly a cq Thr ga t aac As n cat His 30 gg t Gly gat tttgaccctg cagcccctgc atg agc ctc atc gaa Met Ser Leu Ile Giu 1 ict gaa ggt gaa ctc Ser Giu Gly Giu Leu cgt ggc gaa ttc gtg Arg Gly Giu Phe Val acg atg atg aac atc Thr Met Met Asn Ile tac acc ttg gat ggc WO 01/00805 WO 0100805PCTIBOOIOO926 Ile Gly gtg gat Vai Asp aaa tcg Lys Ser gat gcg Asp Ala gct aag Ala Lys atg ggt Met Giy 135 aag cag Lys Gin 150 att ctt Ile Leu atg gtg Met Val atc gtg Ile Val gtg gtc Vai Val 215 Leu Leu Asp Lys Pro Thr Asp Giy Thr Tyr Thr Leu Asp Giy gtg Val1 att Ile ttg Leu cag Gin 120 gag Giu cgc Arg gct Ala atg Met ttt Phe 200 acc Thr ttg Leu ggt Gly aag Lys 105 cg g Arg cgt Arg gtg Val1 ga t Asp gat Asp 185 att Ile atg Met agc S er ttt Phe gaa Giu cg t Arg cat His 140 gct Ala act Th r cac His aac As n ggg Gly 220 gat gat Asp Asp cag aac Gin Asn atg ccc Met Pro 110 gcg qtt Ala Val 125 gag ccc Glu Pro cgc gcg Arg Ala ggt gcg Gly Ala cag ctc Gin Leu 190 cct gag Pro Glu 205 cgc atc Arg Ile gct Ala ttc Ph e atg Met ga a Giu aat Asn t tg' Leu ttg Leu 175 aac As n ctt Leu att Ile ttg Leu aat As n atg Met tta Leu gag Glu gcg Ala 160 ga t Asp aag Ly s gct Ala ggg Gly gcg Ala ct g Le u tat Tyr ttg Leu ct t Leu 145 aac Asn tct Ser gag Giu ga t Asp tct Ser tca tgagccttgc agaatcaatt ctt Ser 230 <210> 334 <211> 230 <212> PRT <213> Corynebacterium glutamicum <400> 334 Met Ser Leu Ile Giu Met Arg Asn Ile Val Lys Thr Tyr Asn Ile Giy 1 5 10 Ser Glu Gly Glu Leu Thr Val Leu His Gly Val Asp Phe His Val Asp 25 Arg Gly Glu Phe Val Ser Val Val Gly Thr Ser Gly Ser Gly Lys Ser WO 01/00805 WO 0100805PCT/IBOO/00926 Thr T yr Ala Leu T yr Le u Le u 145 As n Se r Giu Asp Ser 225 Met Thr Ser Ile Ala Giu 130 Ser Asp Ala Gin Gi u 210 Glu Met Leu His Gly Gi y 115 Met Gly Pro Thr Gly 195 Ser Val1 As n Asp Arg Arg 100 Ile Val1 Gly Gi u Gly 180 Lys Asp Lys Ile Ilie Gly 55 Gly Val Asp 70 Ala Lys Ser Ile Asp Ala Pro Ala Lys Gly Met.Gly 135 Gin Lys Gin 150 Ile Ile Leu 165 Arg Met Val Thr Ile Val Arg Vai Val 215 His Ser 230 Leu Leu Asp Lys Pro Thr Asp Giy Thr Leu Gly Lys 105 Arg Arg Val1 Asp Asp 185 Ile Met Asp Ph e Asn Arg Le u Ala Gi u 170 Ile Th r Val1 Ile Vali Vali Ser Asn Ile 155 Pro Ph e His Asp Ser Phe Giu Arg His 140 Ala Th r His Asn Gly 220 Asp Gin Met Ala 125 Giu Arg Gly Gin Pro 205 Arg <210> 335 <211> 1008 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <222> (101)..(985) <223> RXN00733 <400> 335 acggcgaggt tgtcggtatt ggaacgcaca cgaatttgct gaacacgtgc ggtacctacc gtgaaattgt tgaatcccaa qagactgcgc aggcgcaatc atg agt aat act gca Met Ser Asn Thr Ala 1 ggc ccc cgc ggg cgt tcc cat cag qca gac gcc gcg ccg aat caa aag Giy Pro Arg Gly Arg Ser His Gin Ala Asp Ala Ala Pro Asn Gin Lys 15 gca cag aat ttc gga cca tct gcc aaa agg ctt ttc gga att cta ggc WO 01/00805 WO 0100805PCT/IBOO/00926 Ala Gin Asn Phe Gly Pro Ser Ala Lys Arg Leu Phe Gly Ile Leu Gly cat His gt t Val gtg Val aag Lys gc t Ala ttt Ph e atc Ile qt g Val1 1.50 cac His ctt Le u caa Gin ttg Leu gt a ValI 230 tcc Ser gac Asp gga Gly gtg Val1 gaa Giu tcc Ser gaa Glu gg t Gly 135 caa Gin cgc Arg agc Ser acc Th r gtg Val 215 tcc Ser cag Gin att Ile Gly cta L eu ca g Gin atg Met atc Ile ttg Le u 140 cgg Arg tat Tyr gat Asp atc Ile atc Ile 220 atc Ilie ga a Gi u ttt Ph e cca Pro t ct Ser t tg Le u aac As n ct C Le u 125 ttC P he ctg Leu ttc Phe qtg Val1 act Thr 205 t cc Ser gtg Val1 cag Gin gtt atc Val Ilie tgg ttg Trp Leu aag cgc Lys Arg cag gct Gin Ala ctt gtt Leu Val 110 gga ctg Gly Leu cag gcg Gin Ala cgc atg Arg Met gat tc Asp Ser 175 gat aat Asp Asn 190 tcc cta Ser Leu cca Ctg Pro Leu gtc act Vai Thr tgg aaq Trp Lys 255 ggc cac Giy His gcc gtc Ala Val aaa gcc Lys Ala gct ggt Ala Giy aaa cat Lys His tca ggc Ser Gly 115 ggt gct Gly Ala 130 ctC aac Leu Asn gag gaa Glu Giu cgt ggt Arg Gly caa tcc Gin Ser 195 gtc atc Vai Ile 210 ctc gtg Leu Val gtt gcg Val Ala ggt att Gly Ile gtt aag Vai Lys ctg Leu a cc Thr gcg Al a aat Asn 100 att Ile tat Tyr cg c Arg aaa Lys ga t Asp 180 ctg Leu gg t Giy 9gCg Al a agc Ser ttg Leu 260 gtt Val1 agc S er aac Asn tca Ser ca g Gin ga t Asp ctc Leu atc Ile atc Ile 165 ctg Leu caa Gin gtg Val1 ctg Leu cgt Arg 245 aat Asn ttc Phe 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 gcg cgc ctg gag gaa Ala Arg Leu Glu Giu acc tac tct Thr Tyr Ser WO 01/00805 PCT/IB00/00926 265 270 275 gga cac caa aag gat gtt caa gaa gca ttc gag gaa gaa aat caa gct 979 Gly His Gin Lys Asp Val Gin Glu Ala Phe Glu Glu Glu Asn Gin Ala 280 285 290 tgt gta taaggccagc tttggtgccc agt 1008 Cys Val 295 <210> 336 <211> 295 <212> PRT <213> Corynebacterium glutamicum <400> 336 Met Ser Asn Thr Ala Gly Pro Arg Gly Arg Ser His Gin Ala Asp Ala 1 5 10 Ala Pro Asn Gin Lys Ala Gin Asn Phe Gly Pro Ser Ala Lys Arg Leu 25 Phe Gly Ile Leu Gly His Asp Arg Asn Thr Leu Ile Phe Val Ile Phe 40 Leu Ala Val Leu Ser Val Gly Leu Thr Val Leu Gly Pro Trp Leu Leu 55 Gly Lys Ala Thr Asn Val Val Phe Glu Gly Phe Leu Ser Lys Arg Met 70 75 Pro Ala Gly Ala Ser Lys Glu Asp Ile Ile Ala Gin Leu Gin Ala Ala 90 Gly Lys His Asn Gin Ala Ser Met Met Glu Asp Met Asn Leu Val Pro 100 105 110 Gly Ser Gly Ile Asp Phe Glu Lys Leu Ala Met Ile Leu Gly Leu Val 115 120 125 Ile Gly Ala Tyr Leu Ile Gly Ser Leu Leu Ser Leu Phe Gin Ala Arg 130 135 140 Met Leu Asn Arg Ile Val Gin Ser Ala Met His Arg Leu Arg Met Glu 145 150 155 160 Val Glu Glu Lys Ile His Arg Leu Pro Leu Ser Tyr Phe Asp Ser Ile 165 170 175 Lys Arg Gly Asp Leu Leu Ser Arg Val Thr Asn Asp Val Asp Asn Ile 180 185 190 Gly Gin Ser Leu Gin Gin Thr Leu Ser Gin Ala Ile Thr Ser Leu Leu 195 200 205 Thr Val Ile Gly Val Leu Val Met Met Phe Ile Ile Ser Pro Leu Leu 210 215 220 Ala Leu Val Ala Leu Val Ser Ile Pro Val Thr Ile Val Val Thr Val 225 230 -235 240 WO 01/00805 PCTLBOOIOO926 Val Val Ala Ser Arq Ser Gin Lys Leu Phe Ala Giu Gin Trp Lys Gin 245 250 255 Thr Gly Ile Leu Asn Ala Arg Leu Giu Giu Thr Tyr Ser Giy His Ala 260 265 270 Val Val Lys Val Phe Gly His Gin Lys Asp Val Gin Giu Ala Phe Giu 275 280 285 Giu Glu Asn Gin Ala Cys Val 290 295 <210> 337 <211> 508 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(508) <223> FRXA00733 <400> 337 acggcgaggt tgtcggtatt ggaacgcaca cgaatttgct gaacacgtgc ggtacctacc gtgaaattgt tqaatcccaa qagactgcgc aggcgcaatc atg agt aat act qca 115 Met Ser Asn Thr Ala 1 99C ccc cgc ggg cqt tcc cat cag gca gac gcc gcg ccg aat caa aaq 163 Gly Pro Arg Giy Arg Ser His Gin Ala Asp Ala Ala Pro Asn Gin Lys 15 gca cag aat ttc qqa cca tct gcc aaa agg ctt ttc gga att cta ggc 211 Ala Gin Asn Phe Gly Pro Ser Ala Lys Arg Leu Phe Gly Ile Leu Gly 30 cat qac cgt aac acc tta att ttt gtt atc ttc cta gcc gtc ctg agc 259 His Asp Arg Asn Thr Leu Ile Phe Val Ile Phe Leu Ala Val Leu Ser 45 gtt gga ctt acc gtc ttg ggc cca tgg ttg ctg ggt aaa gcc acc aac 307 Val Gly Leu Thr Val Leu Gly Pro Trp Leu Leu Gly Lys Ala Thr Asn 60 gtg gtg ttt gaa gga ttc cta tct aag cgc atg ccg gct ggt gcg tca 355 Val Val Phe Glu Gly Phe Leu Ser Lys Arg Met Pro Ala Gly Ala Ser 75 80 aag gaa gat atc atc gcg cag ttg cag gct gca qgt aaa cat aat cag 403 Lys Glu Asp Ile Ile Ala Gin Leu Gin Ala Ala Gly Lys His Asn Gin 95 100 gct tcc atg atg gaa gac atg aac ctt gtt cca ggc tca ggc att gat 451 Ala Ser Met Met Giu Asp Met Asn Leu Val Pro Gly Ser Gly Ile Asp 105 110 115 ttt gaa aaa tta gcc atg atc ctc qga ctg gtg atc ggt gct tat ctc 499 Phe Giu Lys Leu Ala Met Ile Leu Gly Leu Val Ile Gly Ala Tyr Leu WO 01/00805 PCTIBOO/00926 120 125 130 att cgt agc 508 Ile Arg Ser 135 <210> 338 <211> 136 <212> PRT <213> Corynebacterium glutamicum <400> 338 Met Ser Asn Thr Ala Gly Pro Arg Gly Arg Ser His Gin Ala Asp Ala 1 5 10 Ala Pro Asn Gin Lys Ala Gin Asn Phe Gly Pro Ser Aia Lys Arg Leu 25 Phe Gly Ile Leu Gly His Asp Arg Asn Thr Leu Ile Phe Val Ile Phe 40 Leu Ala* Val Leu Ser Val Gly Leu Thr Vai Leu Giy Pro Trp Leu Leu 55 Giy Lys Ala Thr Asn Val Val Phe Glu Gly Phe Leu Ser Lys Arg Met 70 75 Pro Ala Gly Ala Ser Lys Giu Asp Ile Ile Ala Gin Leu Gin Ala Ala 90 Gly Lys His Asn Gin Ala Ser Met Met Giu Asp Met Asn Leu Val Pro 100 105 110 Gly Ser Gly Ile Asp Phe Giu Lys Leu Ala Met Ile Leu Giy Leu Val 115 120 125 Ilie Gly Ala Tyr Leu Ile Arg Ser 130 135 <210> 339 <211> 692 <212> DNA <213> Corynebacterium qiutamicum <220> <221> COS <222> (669) <223> RXA00735 <400> 339 ggt ccc acc ggt gcg ggc aag acc aca ttg gtg aat ctg atc atg cqt 48 Gly Pro Thr Gly Ala Gly Lys Thr Thr Leu Val Asn Leu Ile Met Arg 1 5 10 ttc tac gac atc aac agc ggt tcc atc act ctt ggt gaa aca gca caa 96 Phe Tyr Asp Ile Asn Ser Gly Ser Ile Thr Leu Gly Giu Thr Ala Gin 25 gac gcc gtg gat atc cqc acc atq gct aga qaa gat ctg cga tca cga 144 WO 01/00805 WO 0100805PCT/IBOO/00926 Asp Ala Val Asp Ile Arg Thr Met Ala Arg Glu Asp acc Th r ga t Asp gct Al a ga a Giu ggt GI y cg a Arg ga a Giu 145 gCC Al a att Ilie gag Glu ttc atg Met att Ile tcC Ser tac Tyr cgc Arg 115 ctg L eu ttg Leu gtc Val1 atg Met atg Met 195 gcc gtg Val1 ct t Leu aag Ly s gac Asp 100 cag Gin att Ilie att.

Ile atc Ile atq Met 180 gcc Al a ccc Ctg Leu g ca Ala cg t Arg gaa Giu cgt Arg tca Ser aag Lys acg Thr 170 gtg Val1 t gg T rp ttt Phe act Thr ttt Ph e gcc Ala gca Ala t cg Ser Ctg Leu 155 att Ile gagq Glu gag Glu gcc Ala gag Giu gtc Val1 atg Met tt C Ph e gtg Val1 140 cgc Arg cg t Arg cag Gin ttg Leu 192.

240 288 336 384 432 480 528 576 624 gaa tta cag gct gac gga gat cac Phe Asn Ala Pro Ala Lys Giu Giu Leu Gin Ala Gly Asp His tgatgatttc ttcttaggct ttC <210> 340 <211> 223 <212> PRT <213> Corynebacterium giutamicum <400> 340 Gly Pro Thr Gly Ala Gly Lys Thr Thr Leu Val Asn Leu Ile Met Arg 1 5 10 Phe Tyr Asp Ile Asn Ser Gly Ser Ile Thr Leu Gly Glu Thr Ala Gin 25 WO 01/00805 WO 0100805PCT/1B00100926 Asp Th r Asp Al a Glu Gi y Arg Glu 145 Ala Ile Glu Phe Ile Arg Thr Met Ala Arg Glu Asp Leu Arg Ser Arg Le u Tyr Ala Th r Le u Leu Gin Ala 165 Lys Leu Gln Gly Ala Val1 Ile Asp Arq 150 His Asp Glu Gly Glu Arq Asn Leu 125 Asp G In Asp Gly Tyr 205 Thr Glu Se r Leu 110 Ala Th r Asp Al a As n 190 Asn Pro Ala Lys Glu Glu Leu Gin Ala 215 Gly Asp His <210> 341 <211> 1986 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (1963) <223>' RXA00878 <400> 341 cgagattaqg tccgcttcag ttgtggtggc tccgaatctq atgaacaatg atcattccta attcatttac atctttatca aagagagcca ccacctacta atg cga ctt ctt ggt Met Arg Leu Leu Gly 1 cga att tta aaa acc acg tct qcg ctt tgg ccc tac tat ctc gga att Arg Ile Leu Lys Thr Thr Ser Ala Leu Trp Pro Tyr Tyr Leu Gly Ile 15 atc gtc qta tcc att gtg atc gcg gcg ttg tcg ctq ctq tcg ccg ttt Ile Val Val Ser Ile Val Ile Ala Ala Leu Ser Leu Leu Ser Pro Phe WO 01/00805 WO 0100805PCT/IBOO/00926 att Ile aac As n ctg Leu tac Tyr cg C Arg cag Gin atc Ile atc Ile 150 ctg Le u gcg Aia gaa Giu aag Lys ggt Gi y 230 t gg Trp ttc Ph e etc Leu acc Thr ttt Phe atc Ile tat Tyr gjt C Vai a cg Thr 135 acc Th r gca Ala t tg Leu atc Ile gt t Val 215 ggg Gly t qq Trp ctg Leu g ca Ala g ca Ala a gc Ser gtc Val aag Lys acc Thr a tg Met qca Ala ctg Leu 170 aaa L ys gct Ala teca Ser ggC Gly at g Met 250 cac His aca Th r gtt Val1 ttc Ph e 75 atg Met ctg Leu atc Ile Ca g Gin gtg Val 155 gcc Ala cg C Arg aac Asn ttC Ph e aaa Lys 235 ga t Asp Ct 9j Leu ga t Asp act Th r 60 ctc Le u g ca Ala ttq Le u atc Ile agc Se r 140 ctg Le u atg Met tgg Trp ggc Gly gte Val 220 aca Th r act Thr Ctg Le u tcc Ser eg C Arg aat Asn t ct Ser 9 C9 Ala 9CC Ala 125 ttc Ph e att Ile ttg Leu cag Gin C9 C Arg 205 gca Ala gt a Val ctc Le u att Ile att Ile act Th r acg Thr cgt Arg ct g Leu 110 ege Arg tee Ser att Ile ttc Phe aaa Lys 190 ttc Phe gag Glu gcg Al a cg t Arg ttc Phe 270 gtt Val1 att Ile gtg ValI a tg Met 95 c ct Pro ctt Leu aac Asn tee S er ccg Pro 175 tat Tyr get Ala ace Thr att Ile Gly 255 tac T yr tct Se r att Ile atg Met 80 cg C Arg cag Gin gat' Asp aac Asn gcq Al a 160 att Ile gag Glu gag Giu cgc Arg acc Th r 240 gcg Ala cg c Arg gca Ala ttC Ph e acc Thr ca 9 Gin aag Lys cga Arg ttc Ph e 145 att Ile tac Tyr ggc Gly gt t Val gag Glu 225 cgg Arg gca Ala ace Thr gta Val1 tta Leu aac Asn att Ile tat T yr tca Ser 130 ttc Ph e ttc Phe atg Met gag Glu qt C ValI 210 C tg Leu C Cg Pro eta Leu t tg Leu ace Thr get Ala ate Ile et g Leu ttt Phe 115 ate Ile ccc Pro tac Tyr t gg Trp aaa Lys 195 ggC Gly get Ala ca a Gin aat As n cac His 275 g ga Gly tta Leu gg t Gly 9C Ala 100 ga t Asp aac Asn atg Met tgg Trp.

ctg Le u 180 aac As n ca 9 Gln ga t Asp tee Ser ate Ile 260 ggc Gly tet Ser gee Al a gge Gly a eg Th r aat Asn ggc Gly etc Leu c Ct Pro 165 a eg Thr cat His gte ValI ttC Ph e ggt Gly 245 ate Ile cat His 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 WO 01/00805 WO 0100805PCTJIBOO/00926 ttc Ph e ca g Gin gcc Al a 310 gag Gi u act Th r cca Pro ccg Pro g cg Ala 390 ctg Leu gtg Val1 gtg Val1 atc Ile gct Ala 470 tat Tyr a ag Lys ggc gac atg gtc Gly Asp Met Val gtg tac atg atg Val Tyr Met Met 300 ggc tcc cgc gac Gly Ser Arg Asp 315 gcc aat aag gag Ala Asn Lys Giu 330 atc agt gtg ggc Ile Ser Val Gly 345 qaa ttc aaa aac Giu Phe Lys Asn tcc gac gtg tcc Ser Asp Val Ser 380 ggt gaa tcc ggc Gly Giu Ser Gly 395 ctg tac aaa cca Leu Tyr Lys Pro 410 aaa gat ctg act Lys Asp Leu Thr 425 cag gac gcc agc Gin Asp Ala Ser ggt cgc cca ggt Gly Arg Pro Gly 460 gcc aac gca cat Ala Asn Ala His 475 gtc gtc ggt gaa Val Val Gly Glu 490 gtc tct gtg gca Val Ser Val Ala 505 atg ctc atc cag ctt gtc acc atg gcg Met Leu Ile Gin Leu Val Thr Met Ala 285 290 agc Ser tac Tyr ct t Leu acg Th r gt c Val 365 att Ile Gly aac Asn tcc Ser t tg Leu 445 gcc Ala gag Giu cg c Arg cgg Arg tac Tyr ttc Ph e gt c Val1 ccc Pro 350 acc Th r acc Thr gg t Gly agc Ser gag Glu 430 ttc Phe acc Thr ttc Phe gga Gi y gcc Al a 510 ga c Asp 305 atg Met acc Thr ctg Leu tac Tyr cac His 385 a cc Thr ctt Leu cgc Arg tct S er gag Giu 465 gcc Ala ctt Leu aaa Lys tcc Ser gcg Ala ct c Leu ccc Pro ga a Giu 370 ggc Gly ctg Leu g ca Ala gca Ala att Ile 450 atc Ile ttc Phe tct S er ga t Asp gcg Ala cag Gin gcc Ala gct Ala 355 gaa Glu gag Giu gtc Val1 gta Vai tcc Ser 435 gca Al a atc Ile cct Pro ggt Gly gcc Ala 515 ca g Gin cag Gin t ca Ser 340 gga Gly ggc Gly cgc Arg aac Asn tgt C ys 420 gtg Val gaa Giu gaa Giu gaa Glu ggc Gly 500 cca Pro cg c Arg gtc Val 325 ga c Asp gaa Glu aag Lys atc Ile ct t Leu 405 ggC Gly ggt Gly aac As n gt g ValI gga Gly 485 ca g Gin ctt Leu 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 WO 01/00805 ctt gtt ctc gat gaa gcc acc tct Leu Val Leu Asp Giu Ala Thr Ser 520 .525 gca gtc caa 9CC ggt ttg gaa cag Ala Val Gin Ala Gly Leu Giu Gin 535 540 atg atc gcc cac cgc ctg tcc acc Met Ile Ala His Arg Leu Ser Thr 550 555 acc atc caa aac gga cgg gtt gaa Thr Ilie Gin Asn Gly Arq Val.Giu 570 gca gtc tca ggc ggt atc tat tcc Ala Vai Ser Giy Gly Ile Tyr Ser 585 aca gca qaa gcc gac cgg gag cgt Thr Ala Giu Ala Asp Arg Glu Arg 600 605 gat gca cca gct gaa gaa gag gac Asp Ala Pro Ala Giu Giu Giu Asp 615 620 <210> 342 (211> 621 ':212> PRT <213> Corynebacterium giutamicur <400> 342 Met Arg Leu Leu Giy Ar Ile Leu 1 Tyr Tyr Leu Giy Ile Ile Val Vai Leu Leu Ser Pro Phe Ile Leu Arg 40 Ala Val Thr Gly Ser Asn Thr Val Phe Leu Ala Leu Ala Leu Phe Vai Thr Asn Ile Gly Gly Tyr Ile Gly Gin Ile Leu Ala Thr Arg Tyr Tyr 100 Lys Tyr Phe Asp Asn Gin Val Thr 115 120 Arg Ser Ile Asn Gly Ile Thr Gin 130 135 gca ctg gat acc aag tct gag Ala Leu Asp Thr Lys Ser Giu 530 ctg atg gaa aac cgc acc acc Leu Met Giu Asn Arg Thr Thr 545 atc gca ggc gtc gat acc atc Ile Ala Giy Val Asp Thr Ile 560 gag gtc gga tct cct acc gag Giu Val Gly Ser Pro Thr Giu 575 580 gaa ctg ctg cgc ctg acc aac Giu Leu Leu Arg Leu Thr Asn 590 595 ctg cgc gcc ttt ggt ttc act Leu Arg Ala Phe Gly Phe Thr 610 tagccccgcg aaaqaacaat ccc PCTIBOOIOO926 cag 1699 Gin tta 1747 Leu gtg 1795 Val1 565 ctc 1843 Leu tcc 1891 Se r ggc 1939 Gly 1986 Ala Ala Se r Arg As n Se r Ala Ala 125 Ph e WO 01/00805 WO 0100805PCTIIBOO/00926 Phe 145 Ile T yr Gly Val1 Giu 225 Arg Ala Th r Leu Asp 305 Met Thr Leu Tyr His 385 Thr Leu Arg Phe Ph e Met Giu Val1 210 Leu Pro Leu Leu Val 290 Ser Ala Leu Pro Glu 370 Gly Le u Ala Ala Pro T yr Trp Lys 195 Gly Ala Gin Asn His 275 Thr Ala Gin Ala Ala 355 Giu Glu Val1 Val1 Ser 435 Met Leu Ile 150 Trp Pro Leu 165 Leu Thr Ala 180 Asn His Glu Gin Val Lys Asp Phe Gly 230 Ser Gly Trp 245* Ile Ilie Phe 260 Gly His Phe Met Ala Gin Gin Arg Ala 310 Gin Val Glu 325 Ser Asp Thr 340 Gly Glu Pro Gly Lys Pro Arg Ilie Ala 390 Asn Leu Leu 405 Cys Gly Vai 420 Val Gly Val Thr Met Val Ala Val Leu Ile Ile Ser Ala 155 160 Aia Leu Ile Val 215 Gly Trp Leu Thr Gin 295 Ilie Pro Pro Ala Val 375 Leu Leu Asp Val Ile Thr Asp 200 Vai Ar g His Ala Ile 280 Pro Ala Thr Arg Met 360 Ile Val1 Gly Val1 Phe 440 Ile Giu Giu Arg Th r 240 Ala Arg Gin Val Val1 320 Ala Ala Al a Arg S er 400 Ser Leu Gly Ser Ilie Ala Giu Asn Ilie Ala Tyr Gly Arg Pro 450 455 Ala Thr Arg Glu WO 01/00805 WO 0100805PCT/IBOO/00926 Glu Ile Ile Glu Val Ala Ph e Se r Asp Lys 530 Arg Asp Pro Le u Gly 610 Pro Gly Ala 515 Ser Thr Thr Th r Thr 595 Phe G lu Gly 500 Pro Giu Thr Ile Glu 580 As n Thr Ala Val Val1 505 Asp Ala His As n Gly 585 Ala Ala Asn Val1 490 Ser Glu Gly Arg Gly 570 Gly Asp Glu Ile Leu 495 Met Leu Met Ala Val1 575 L eu Arg Ser 480 Lys Leu Asp Glu Gly 560 Gly Le u Ala <210> 343 <211> 1713 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1690) <223> RXN01191 <400> 343 cgctgctttc acgcaactga aaccgcaccg gatcaagtta cgtgttggtg gccgtcgcgg ggttggtagg gccctgggcg ga t Asp ctg Leu ggc Gi y cg c gca Ala gct Ala a tg Met ttg gac Asp agc Ser ccg Pro ttg tttggggttg ttctttgtgg gtg ggt gga ctc gtc Val Gly Gly Leu Val 1 gtt gta qtg ttc geg Val Val Val Phe Ala ctg ggc acg tgg tgg Leu Gly Thr Trp Trp gcg atc gcg ggg ctg Ala Ile Ala Gly Leugat gcg aac acg att WO 01/00805 Arg Glu Asp Val Leu Arg Ala Ala Val Ser Leu PCTJIBOO/00926 Ala Asn Thr Ile gaa acg Glu Thr cgg gag Arg Glu gcg ggc Ala Gly tgg cgc Trp Arg acg ttg Thr Leu 135 cgc gag Arg Glu 150 ggC gcg Gly Ala cgt atc Arg Ilie ttc agg Phe Arg atc acc Ile Thr 215 gat ctg Asp Leu 230 cga ctc Arg Leu caa tcc Gin Ser gca tcg Ala Ser gcg Al a gtg Val1 ttt Phe ctc Leu 120 cgc Arg gcc Ala gaa Glu ga c Asp ttc Phe 200 ctc Leu gtc Val ttc Phe gcc Ala aac Asn 280 ggg Gly tcc Se r acc Th r 105 ggc Gly gtc Val1 ttt Phe acc Th r gca Ala 185 ct C Leu gtg Val1 acc Thr gq t Gly agc Ser 265 ca g Gin cgc Arg act Thr gtg Val1 ctt Leu tat Tyr ggg Gly ttg Leu 170 gcc Ala aca Th r ctc Leu gtC Val cca Pro 250 gca Ala gtc Val 99c Gly 75 gcg Al a qtg Val gt c Val tta L eu gtg Val 155 cg C Arg tcc Ser tgg Trp atc Ile ggc Gly 235 atc Ile t cg Ser agc Se r g ac Asp gcg Ala att Ile ggt Gly

CCC

Pro 140 cgc Arg gct Ala ggc Gly gca Ala ttg Le u 220 gca Ala ggC Gly ct g Leu ggc Gly gtg ValI agc S er tcC S er ttg Leu 125 cgc Arg a cg Thr ttc Phe gaa Glu ttt Phe 205 ggC Gly gt c Val1 a cg Th r atc Ile acc Thr 285 att Ile acc Thr g cg Ala 110 gtc Val1 tca Se r cag Gin cg C Arg gcc Ala 190 tcC Ser acc Thr tca Ser ctc Leu cg C Arg 270 t cg Ser tcg Ser gtg Val1 95 ttt Phe gcg Ala gg t Gly Cgg Arg gca Al a 175 cg C Arg cg C Arg ggC Gly acc Th r gtg Val1 255 atg Met c cg Pro tat Tyr cgt Arg 80 g tg Val1 Gly atc Ile c cg Pro ct t Leu 1 60 gaa Glu gac Asp aac Asn ttt Phe g cc Ala 240 ggc Gly gtg ValI g cg Al a cac His Asp atC Ile ccg Pro atg Met c cg Pro ctt Leu 145 gt c Val gat Asp att Ile aac As n tac Tyr 225 g ca Al a atg Met ggc Gly t ct Ser act Th r g cg Ala ctg Leu gcg Ala C tg Le u 130 tat Tyr qgc Gly a ca Thr tcc Ser cgc Arg 210 ctg Leu ctg Leu ttc Phe qt t Val gcc Ala 290 g ca Ala ga t Asp atg Met gcg Ala 115 tat T yr a cg Th r gca Al a qaa Glu att I I e 195 g cg Al a gtc ValI atc Ile t CC Ser att Ile 275 agc Ser

CCC

Pro ga t Asp gtg Val 100 gt t ValI tgg Trp cg t Arg gtc Val1 tta Leu 180 tct S er gaa Giu aac Asn ttc Phe gac Asp 260 aac As n acC Th r gt c Val t cg S er cag Gin ga t Asp acc Thr gag Glu ga a Glu 165 aag Lys gtt ValI tgc Cys atc Ile cac His 245 atc Ile gcg Ala gct Ala atc Ile 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 tta acg ctt ttc Leu Thr Leu Phe gac qtC Asp Val tcc cac cac Ser His His WO 01/00805 WO 0100805PCT/IBOO/00926 aag aat gca Lys Asn Ala 310 ggt gcg acc Gly Ala Thr ctq ctc agc Leu Leu Ser tct aac gtc Ser Asn Val 360 caa gaa atc Gin Giu Ile 375 gca cqc ccc Ala Arg Pro 390 att ggt gat Ile Gly Asp gtq ggt gat Val Giy Asp qcg ctt gct Ala Leu Ala 440 gaa gca acg Glu Ala Thr 455 gca gcc ctt Ala Ala Leu 470 cgc ctc aac Arg Leu Asn gga gaa atc Gly Glu Ile ggc cga tat Gly Arg Tyr 520 t cc Ser ggc Gly cca Pro 345 gaa Glu cac His ga a Giu tcc Ser ggc Giy 425 cqc Arg gct Ala aaa Lys caa Gin at a Ile 505 gaa Glu gtg Val1 gct Ala 330 act Thr ccq Pro t gc C ys qcc Ala t gg Trp 410 gct Ala gta Val1 gaa Glu gt c Val1 gcg Al a 490 qa a Glu cag Gin 315 gq t Gly t cc Se r gaa Giu ttc Phe acc Thr 395 t tg Leu ttc Ph e cat His t ca Ser act Thr 475 aaa Lys t ct Ser cca Pro a cg Thr gtg Val1 350 cgc Arg t ct Ser gac Asp tta Leu a cc Thr 430 gac Asp ga t Asp aga Arg ga t Asp cat His 510 gqg Gly ctc Le u 335 gc t Al a cag Gln gt t Val1 atc Ile ccg Pro 415 tct Se r cta Leu cat His t ca Ser cgc Arg 495 gaa Glu qa a Giu 320 gcc Ala ctc Leu aaq Lys tta Le u ca c His 400 caa Gin gtg Val1 gca Ala g ca Ala gcc Al a 480 atc Ile gag Glu cac His ctc Leu ggc Gly atc Ile ga t Asp 385 gcc Ala gg c Gly gaa Glu atc Ile aaa Lys 465 atc Ile atc Ile ctt Le u gcc att- gti Ala Ile Va.

32' gcq qca gg, Ala Ala Gl' 340 tcg agt tt, Ser Ser Phf 355 atg gtc agc Met Val Se: ctt cgt at( Leu Arg Ilf ctc gcc gal Leu Ala Asi 4 0! gac acc at( Asp Thr 1il 420 cag atc ati Gin Ile Mel 435 atc ctt gal Ile Leu As 1 ctt gaa gal Leu Glu Asj gtg gct cai Vai Ala HiL 4 8' atg gac tci Met Asp Se: 500 gcg atc gg4 Ala Ile G1, 515 taattagcca 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1700 caa ctg tgg Gin Leu Trp gcg tgg tct gcg Ala Trp Ser Ala cccaagacca cqc 11 1713 WO 01/00805 <210> 344 <211> 530 <212> PRT <213> Corynebacterium giutamicum <400> 344 Val Gly Gly Leu Val Asp Lys Leu Leu PCT/EBOO/00926 1 Val Le u Ala Asp Ile Pro Met Pro Leu 145 Val1 Asp Ile Asn T yr 225 Ala Met Gly Ser Val Gly Ile Ala Ala Leu Ala Leu 130 Tyr Gly Thr Ser Arg 210 Leu Leu Ph e ValI Ala Val1 Th r Ala As n Asp Met Al a 115 Tyr Thr Ala Glu Ile 195 Ala Val1 Ile Se r Ile 275 Ser Phe Trp Gly Thr Asp ValI 100 ValI Trp Arg Val1 Leu 180 Ser Glu Asn Phe Asp 260 Asn Th r 5 Ala Trp Leu Ilie Ser Gin Asp Thr Giu Giu 165 Lys ValI Cys Ile His 245 Ilie Ala Ala Leu Gly Ar g Glu 70 Arq Ala Trp Thr Arg 150 Gly Arg Phe Ile Asp 230 Arg Gin Ala Leu Leu Ser Glu 55 Th r Glu Gly Arg Leu 135 Glu Ala Ile Arg Th r 215 Le u Leu Ser Ser Thr Ilie Ala 40 Asp Ala Val1 Phe Leu 120 Arg Ala Glu Asp Ph e 200 Le u Val1 Ph e Ala As n 280 Leu Val1 25 Leu Val1 Gly Ser Thr 105 Gly ValI Ph e Thr Ala 185 Leu ValI Thr Gly Ser 265 Gin Phe Ala 10 Ala Met Leu Arg Th r 90 Val1 Leu Tyr Gly Leu 170 Ala Thr Leu Val1 Pro 250 Ala Val Asp Th r Gi y Al a Arg GI y 75 Al a Val Val1 Leu Val1 155 Arq Ser Trp Ile Gly 235 Ile Ser Ser Val1 Pro Gly Arg Al a Asp Al a Ile Gly Pro 140 Arg Al a Gly Ala Leu 220 Ala Gi y Leu Gly Ser Ser Val1 Ala Al a Val1 Ser Ser Le u 125 Arg Th r Ph e Glu Phe 205 Gly Val Thr Ile Thr 285 His Met Val1 L eu Val Ile Thr Ala 110 Val S er Gln Arg Al a 190 Ser Thr Ser Leu Arg 270 Ser His Arg Ser Glu Se r Ser Val Ph e Ala Gly Arg Ala 175 Arg Arg Gly Thr Val 255 Met Pro T yr Asp S er Pro Leu Arg Val Gly Ile Pro Leu 160 Giu Asp Asn Phe Ala 240 Gly Val Ala His WO 01/00805 PCTABOO/00926 290 295 300 Thr Ala Pro Val Ile Lys Asn Ala Ser Val Gin Leu Glu Pro Gly Glu 305 310 315 320 His Ile Ala Ile Vai Gly Ala Thr Gly Ala Gly Lys Ser Thr Leu Ala 325 330 335 Leu Ile Ala Ala Gly Leu Leu Ser Pro Thr Ser Gly Gin Val Ala Leu 340 345 350 Gly Gly Ser Ser Phe Ser Asn Val Giu Pro Glu Ala Leu Arg Gin Lys 355 360 365 Ile Ala Met Val Ser Gin Giu Ile His Cys Phe Arg Gly Ser Val Leu 370 375 380 Asp Asn Leu Arg Ile Ala Arg Pro Glu Ala Thr Asp Ala Asp Ile His 385 390 395 400 Ala Val Leu Ala Asp Ile Gly Asp Ser Trp Leu Giu Arg Leu Pro Gin 405 410 415 Gly Ilie Asp Thr Ile Val Gly Asp Gly Ala Phe Arg Leu Thr Ser Val 420 425 430 Glu Asn Gin Ile Met Ala Leu Ala Arg Val His Leu Ala Asp Leu Ala 435 440 445 Ile Val Ile Leu Asp Giu Ala Thr Ala Giu Ser Gly Ser Asp His Ala 450 455 460 Lys Gin Leu Glu Asp Ala Ala Leu Lys Val Thr Glu Asn Arg Ser Ala 465 470 475 480 Ile Ile Val Ala His Arg Leu Asn Gin Ala Lys Thr Ala Asp Arg Ile 485 490 495 Ile Val Met Asp Ser Giy Gu Ile Ile Giu Ser Gly Thr His Glu Giu 500 505 510 Leu Arg Ala Ilie Gly Gly Arg Tyr Glu Gin Leu Trp Thr Ala Trp Ser 515 520 525 Ala Arg 530 <210> 345 <211> 1430 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1407) <223> FRXA01191 <400> 345 gtg agt ttg gat gcg aac acg att gaa acg gcg ggg cgc ggc gac gtg 48 Val Ser Leu Asp Ala Asn Thr Ile Glu Thr Ala Gly Arq Gly Asp Val WO 01/00805 WO 0100805PCT/IBOO/00926 att Ile acc Thr gcg Ala gtC Val t ca Ser ca g Gin c9 C Arg gcc Ala tcc Ser 145 acc Th r t ca Ser ctc Leu cg C Arg t cg Ser 225 cac His gcg Ala ctg Leu g cg Ala ctg Leu tat Tyr 9gc Gly a ca Thr t CC Ser cgc Arg ctg Leu 165 ct g L eu ttc Phe gtt Val1 9C Ala 9 ca Al a 245 ga t Asp atg Met gcg Al a tat Tyr 70 a cg Th r gca Ala gaa Giu att Ile g cg Ala 150 gtC Val atc Ile tcC S er att Ile agc Ser 230

CCC

Pro ga t Asp gtg Val1 gtt Val1 tgg Trp cg t Arg gt c Val tta Leu tct Ser 135 gaa Glu aac Asn ttc Phe ga C Asp aac As n 215 acc Th r gt C Val1 t cg S er cag Gin qa t Asp acc Thr gag Gl u gaa Giu aag Lys 120 gt t ValI tgc Cys atc Ile cac His atc Ile 200 g cg Ala gct Ala atc Ile gag Giu gg C Gly cg C Arg ttg Leu gag Giu 90 gcg Ala atc Ile agg Arg acc Thr ctg Leu 170 Ct C Le u tcc S er t C9 Ser a cg Thr aat As n 250 gtg Val1 ttt Phe ctc Leu CgC Arg 75 gCC Ala gaa Giu gaC Asp ttC Ph e ctc Leu 155 gtC Val ttC Phe 9CC Ala aac Asn Ct t Leu 235 gca Al a tcc Ser acc Thr 99 C Gly gtC ValI ttt Phe acc Thr gca Ala ctc Leu 140 gtg Val1 acc Th r 99 t Gly agc Ser Cag Gin 220 ttc Ph e tcC Ser act Thr gtg Val1 Ctt Leu tat Tyr 999 Gly t tg Leu 9CC Ala 125 aca Thr ctC Leu gtC Val1 cca Pro gCa Ala 205 9t C Val1 gaC Asp gtg Val1 g9 Ala gtg ValI gtc Val1 tta Leu gtg Val1 C9 C Arg 110 tcc S er tgg Trp atc Ile 99C Gly atc Ile 190 t C9 Ser agc Ser gtc ValI Cag Gin gCg Ala a't t Ile ggt Gly

CCC

Pro cqc Arg gct Ala 9gC Gly gca Aia t tg Leu gCa Ala 175 99 C Gi y ct g Leu 99 C Gly tC Ser Ctg Leu 255 a 9C Ser t CC Ser ttg Le u C9C Arg acg Thr ttC Ph e ga a Giu ttt Phe 99 C Gly 160 gtC Val1 a cg Th r atc Ile acc Thr caC His 240 gaa Glu 96 144 192 240 288 336 384 432 480 528 576 624 672 720 768 WO 01/00805 WO 0100805PCT[EBOO/00926 cca ggg gc Pro Gly G] ac9 Ctc 9( Thr Leu A] gtg gct ct Val Ala LE 290 cgc cag a Arg Gin L~ 305 tct gtt Ut Ser Val L gac atc c Asp Ilie H~ tta ccq c i Leu Pro G 3C acc tct g1 Thr Ser V 370 gac cta gj Asp Leu A: 385 qat cat 9g Asp His A aga tca 9g Arg Ser A.

gat cgc al Asp Arg I: 4; cat gaa g~ His Glu G.

450 gcg tgg t Ala Trp Si 465 <210> 346 <211> 469 <212> PRT ia cac atc Lu His Ilie 260 :c ctc att la Leu Ilie :c ggc gga u Giy Gly ~g atc: gcg s5 Ilie Aia :a gat aat u Asp Asn 325 1C gcc gtt is Ala Vai 340 ~a ggc ata Ln Giy Ilie :g gaa aac al Giu Asn ca atc qtc la Ile Vai ca aaa cag la Lys Gin 405 cc atc atc la Ilie Ile 420 tc atc 9tc le Ile Vai 35 39 ctt cga lu Leu Arg ct gcg cgc er Aia Arg 9cc att gtg ggt 9cg acc: ggc gct ggt aaa agc Aia Ile Val gCg Ala t cg S er at 9 Met 310 Ctt Leu CtC Le u ga C Asp ca 9 Gin atc Ile 390 ctt Leu gtg Val1 atg Met 9 C9 Ala qca Aia agt S er 295 gtC Val Cgt Arg

C

Ala a CC Th r atc Ile 375 ctt Leu gaa Giu 9Ct Aia gac Asp atc Gly 265 ctg Leu tct Ser caa Gin gca Ala att Ilie 345 gtg Vali Ala ga a Giu gca Aila CgC Arg 425 gga Giy ggt Aia Thr Giy Ala Giy Lys Ser 270 ct C Le u aac Asn ga a Giu cCC Arg 330 99t Giy 99 t Giy Ctt Leu 9 Ca Aia gCC Ala 410 ct C Leu gaa.

Giu cga act Thr 285 CCg Pro tgC Cys

C

Al a t 99 Trp gct Ala 365 gt a Vai ga a Giu gtC Vali 9 C9 Ala ga a Giu 445 caa Gin t C Ser ga a Giu ttC Phe 8CC Thr t tg Leu 350 ttC Ph e cat His tca Ser act Thr aaa Lys 430 tct Ser ctg Le u 999 Gly gca Ala Cga Arq gat Asp 335 gag Glu cgt Arg ttg Leu 99 C Gly gaa Giu 415 aCC Th r 99 a Gly t9g T rp 816 864 912 960 1008 1056 1104 1152 1200 1248 1296 1344 1392 Ile Giy Gly Arg Tyr Giu 455 460 taattagcca cccaagacca c9c 1430 WO 01/00805 PCT/IB0/00926 <213> Corynebacterium glutamicum <400> 346 Val Ser Leu Asp Ala Asn Thr Ile Glu Thr Ala Gly Arg Gly Asp Val 1 5 10 Ile Ser Arg Ile Ala Asp Asp Ser Arg Glu Val Ser Thr Ala Ala Ser 25 Thr Val Val Pro Leu Met Val Gin Ala Gly Phe Thr Val Val Ile Ser 40 Ala Phe Gly Met Ala Ala Val Asp Trp Arg Leu Gly Leu Val Gly Leu 55 Val Ala Ile Pro Leu Tyr Trp Thr Thr Leu Arg Val Tyr Leu Pro Arg 70 75 Ser Gly Pro Leu Tyr Thr Arg Glu Arg Glu Ala Phe Gly Val Arg Thr 90 Gin Arg Leu Val Gly Ala Val Glu Gly Ala Glu Thr Leu Arg Ala Phe 100 105 110 Arg Ala Glu Asp Thr Glu Leu Lys Arg Ile Asp Ala Ala Ser Gly Glu 115 120 125 Ala Arg Asp Ile Ser Ile Ser Val Phe Arg Phe Leu Thr Trp Ala Phe 130 135 140 Ser Arg Asn Asn Arg Ala Glu Cys Ile Thr Leu Val Leu Ile Leu Gly 145 150 155 160 Thr Gly Phe Tyr Leu Val Asn Ile Asp Leu Val Thr Val Gly Ala Val 165 170 175 Ser Thr Ala Ala Leu Ile Phe His Arg Leu Phe Gly Pro Ile Gly Thr 180 185 190 Leu Val Gly Met Phe Ser Asp Ile Gin Ser Ala Ser Ala Ser Leu Ile 195 200 205 Arg Met Val Gly Val Ile Asn Ala Ala Ser Asn Gin Val Ser Gly Thr 210 215 220 Ser Pro Ala Ser Ala Ser Thr Ala Leu Thr Leu Phe Asp Val Ser His 225 230 235 240 His Tyr His Thr Ala Pro Val Ile Lys Asn Ala Ser Val Gin Leu Glu .245 250 255 Pro Gly Glu His Ile Ala Ile Val Gly Ala Thr Gly Ala Gly Lys Ser 260 265 270 Thr Leu Ala Leu Ile Ala Ala Gly Leu Leu Ser Pro Thr Ser Gly Gin 275 280 285 Val Ala Leu Gly Gly Ser Ser Phe Ser Asn Val Glu Pro Glu Ala Leu 290 295 300 Arg Gin Lys Ile Ala Met Val Ser Gin Glu Ile His Cys Phe Arg Gly WO 01/00805 305 310 315 Ser Val Leu Asp Asn Leu Arg Ile Ala Arg Pro 325 330 Asp Ile His Ala Val Leu Ala Asp Ile Gly Asp 340 345 Leu Pro Gin Gly Ile Asp Thr Ile Val Gly Asp 355 360 Thr Ser Val Glu Asn Gin Ile Met Ala Leu Ala 370 375 Asp Leu Aia Ilie Val Ile Leu Asp Glu Ala Thr 385 390 395 Asp His Ala Lys Gin Leu Glu Asp Ala Ala Leu 405 410 Arg Ser Ala Ile Ile Val Ala His Arg Leu Asn 420 425 Asp Arg Ile Ilie Val Met Asp Ser Gly Glu Ile 435 440 His Glu Glu Leu Arg Ala Ile Gly Gly Arg Tyr 450 455 Ala Trp Ser Ala Arg 465 <210> 347 <211> 1047 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (i01)..(1024) <223> RXN01212 <400> 347 tttagaagcc acatgacata tgtcatgaaa attatgtgca catatggctc taccagcgcc aatgcgaagt aggaagaatt Ala Trp Aia 365 Val Giu Val Al a Giu 445 Gin Thr Leu 350 Phe His Ser Th r Lys 430 Ser Le u Asp 335 Giu Arg Leu Gly Glu 415 Thr Gly Trp PCTJIBOO/00926 320 Ala Arg Leu Aia Se r 400 As n Ala Thr Th r aca cca gca Thr Pro Ala acc gca gtc Thr Ala Val ggt ctg ctc Giy Leu Leu atc gac gta aca gac Ile Asp Val Thr Asp aag ggc ctg aat ttc Lys Giy*Leu Asn Phe ggc acc aac 999 gcc Giy Thr Asn Giy Ala 45 ctc Le u cat His 30 ggc Gly a ga Arg cag Gin acc Th r aagtgcagta atactcctga atg cct atg aca acq Met Pro Met Thr Thr 1 acc tac ggc gac tac Thr Tyr Gly Asp Tyr cgc gqt gaa gta ttt Arg Gly Giu Val Phe tcc acc ttq gaa gtc Ser Thr Leu Giu Val WO 01/00805 WO 0100805PCT/IBO0/00926 atc Ile ct Leu atg Met a ig Met gig ValI g cg Ala ctt Leu 150 gac Asp cag Gin gaa Giu gtg Val1 agt Ser 230 gci Aia ctg Le u ga a Giu ga c Asp ct g Le u gac Asp ct Le u cii Le u 135 ggc Gly cca Pro cq c Arg tc Phe ga a Giu 215 tc Phe gaa Giu cag Gin icc Ser gcc Ala ggC Gly cac His gi c Val ggc Gly i ca Se r a gg Arq 170 acc Th r ga c Asp tig Leu cg i Arg cgc Ar g 250 acc Th r gca Ala ga c Asp 75 gqC Gly gg c Gly gac Asp gaa Glu ati Ile 155 cgc Arg aig Met cgg A rg gati Asp ggc Gly 235 gac Asp tia Leu ccc Pro cqc Arg ctg Leu acc Th r ci c Leu caa Gin 140 t ig Leu cac His aig Met at Ile ga a Giu 220 ggg Gly aac Asn gaa Glu agc Ser gcg Ala cca Pro igc Cys cia Le u 125 cga Arg tic Ph e acc Th r ci g Leu gcc Ala 205 ci g Le u cag Gin aac Asn ata Ile icc Ser aic Ile ica Ser a cgj Thr 110 cac His cgc Arg cic Leu t gg Trp acc Thr 190 aic Ile gig Val1 gig Val1 cac His cii Leu 270 cgc Arg gag Giu gic Val1 gcc Ala gic Val1 130 gcc Ala acc Th r cig Le u cig Leu ggi Gly 210 aag Lys gic Val1 gcc Ala g ca Ala icc Ser ggc Gly gaa Giu 100 aaa Lys gi c Val1 gca Ala gg c Gly cig Leu 180 g aa Giu aic Ile aic Ile a gi Ser a cc Th r 260 acc Th r ggg Gly aic Ile acc Thr gati Asp gg c Gly cig Le u ci c Leu 165 aaa Lys gc c Ala gca Ala aic Ile ggg Gly 245 acc Thr ggg Gly 307 355 403 451 499 547 595 643 691 739 787 835 883 931 aic gcg cig gaa ggc iic gci gca Ile Ala Leu Giu Gly Phe Ala Ala 280 285 aaa ccc gcc acc tig gaa icc gta Lys Pro Ala Thr Leu Giu Ser Val 290 WO 01/00805 ttc atq gac atc gcc tca ctc gag aac acc tcg ctg caa acc gcc Phe Met Asp Ile Ala Ser Leu Giu Asn Thr Ser Leu Gin Thr Ala 295 300 305 tagaatcttt aaggaqacca caa <210> 348 <211> 308 <212> PRT <213> Corynebacterium giutamicum PCTIIBOO/00926 1024 1047 <400> 348 Met Pro Met Thr Thr Thr Pro Ala Ile Gly Giu Leu Ile Leu Ala Ile 115 Lys C ys Thr Asp Giu 195 Giu Ser L eu Asp Val Giu Se r Gly Glu 100 Ly s Val Al a Gly Le u 180 Glu Ile Ile Se r Tyr Phe Val Gly Ile Th r Asp Gly Leu Leu 165 Lys Ala Ala Ile Gly 245 Ala Leu Giu Asp Leu Asp Le u Leu 135 Gly Pro Arg Phe Glu 215 Phe Giu Val1 Leu 40 Gly Pro Gin Met Ala 120 Ser Asp Giu Gly Leu 200 Gly ValI Ile Lys 25 Gly Leu Va 1 Se r Trp 105 Asp Gly Pro Ser ValI 185 Cys Th r Leu Ile Asp 10 Gly Thr Ser Ala Gly 90 His ValI Gly Ser A r c 170 Thr Asp Leu Arg Arg 250 Val1 Leu Asn Ala Asp Gly Gl y Asp Gl u Ile 155 Arg Met Arg Asp Gly 235 Asp Thr As n Gly Pro Arq Leu Thr Leu Gin 140 Leu His Met Ile Glu 220 Giy As n Asp Phe Ala Ser -Ala Pro Cys Leu 125 Arg Phe Th r Le u Ala 205 Leu Gin As n Le u His Giy Ser Ile Se r Thr 110 His Arg Le u Trp Th r 190 Ile Val Val1 His Val1 Val1 Lys Gly Leu Gin Tyr Arg Leu Asp Gin 175 Thr Met Ala Giu Val1 255 Arg Gin Th r Th r Arg Leu Pro Glu Asp Glu 160 Leu His Asn Arq Leu 240 Arg WO 01/00805 PCTIIBOO/00926 Ile Ala Thr Thr Thr Leu Gin Gin His Thr Leu Giu Ile Leu Thr Trp 260 265 270 Ala Ala Glu Thr Gly le Ala Leu Glu Gly Phe Ala Ala Lys Pro Ala 275 280 285 Thr Leu Glu Ser Val Phe Met Asp Ile Ala Ser Leu Glu Asn Thr Ser 290 295 300 Leu Gin Thr Ala 305 <210> 349 <211> 8i3 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> <223> FRXA01212 <400> 349 ggc ctg aat ttc cat gta cag cgc ggt gaa gta ttt gqt ctg ctc ggc 48 Gly Leu Asn Phe His Vai Gin Arg Gly Giu Val Phe Gly Leu Leu Gly 1 5 10 ace aac ggg gcc ggc aaa acc tec acc ttg gaa gte atc gaa gga ctt 96 Thr Asn Gly Ala Gly Lys Thr Ser Thr Leu Giu Val Ile Giu Gly Leu 25 tec gca ccc age tee ggc acc gtg cgc atc tce gqq ctt gae ccc gtt 144 Ser Ala Pro Ser Ser Giy Thr Val Arg Ile Ser Gly Leu Asp Pro Vai 40 gec gac cqe qcg atc etg cgc ccc gag ctc ggc ate atg etg caa tca 192 Ala Asp Arg Ala Ile Leu Arg Pro Glu Leu Gly Ile Met Leu Gln Ser 55 gge ggc ctg cca tca eag ctc acc gte gcc gaa acc atg gac atg tgg 240 Gly Gly Leu Pro Ser Gin Leu.Thr Val Ala Giu Thr Met Asp Met Trp 70 75 cac gge ace tge aeg tat ceg ege gee att aaa gat gtg ett gee gae 288 His Gly Thr Cys Thr Tyr Pro Arg Ala Ile Lys Asp Val Leu Ala Asp 90 gte gac etc eta cae ege gaa aac gte aag gte gge geg ett tee gga 336 Val Asp Leu Leu His Arg Glu Asn Val Lys Vai Gly Ala Leu Ser Gly 100 105 110 gge gaa caa ega ege ctt gat ttg gee tgc gea etg ctt ggc gae eec 384 Gly Giu Gin Arg Arg Leu Asp Leu Ala Cys Ala Leu Leu Gly Asp Pro 115 120 125 tea att ttg ttc cte gac gaa eec aee aec ggc etc gae eca gaa tet 432 Ser Ile Leu Phe Leu Asp Giu Pro Thr Thr Gly Leu Asp Pro Glu Ser 130 135 140 agg ege cac ace tgg eaa etc ctg etg gae ctg aaa eag ege gge gte 480 WO 01/00805 .PCTIIBOO/00926 Arg Arg His Thr Trp Gin Leu Leu Leu Asp Leu Lys Gin Arg Gly Val 145 150 155 160 acc atg atg ctg acc aec cac tac ctg gag gaa gcc gaa ttc ctc tgc 528 Thr Met Met Leu Thr Thr His Tyr Leu Giu Giu Ala Glu Phe Leu Cys 165 170 175 gac cgg att gcc atc atg aac qcc ggt gag atc qea gtg gaa ggc acc 576 Asp Arg Ile Ala Ile Met Asn Ala Gly Giu Ile Ala Val Giu Gly Thr 180 185 190 ttg gat gaa etg gtg gcc ege gag aag tcg atc atc agt tte gtg etg 624 Leu Asp Glu Leu Val Ala Arq Giu Lys Ser Ile Ile Ser Phe Val Leu 195 200 205 egt ggc ggg cag gtg gag ttg ceg gte ttg agt ggg get gaa atc atc 672 Arg Gly Gly Gin Val Glu Leu Pro Val Leu Ser Gly Ala Gu Ile Ile 210 215 220 cge gac aac aac cac gtc ege atc gee ace acc acc etg eag cag cac 720 Arg Asp Asn Asn His Val Arg Ile Ala Thr Thr Thr Leu Gin Gin His 225 230 235 240 acc tta gaa ata ett aec tgg get gca gag ace ggg ate geg ctg gaa 768 Thr Leu Glu Ile Leu Thr Trp Ala Ala Giu Thr Gly Ile Ala Leu Giu 245 250 255 ggc tte get gca aaa ccc gec ace ttg gaa tee gta ttc atg gac 813 Gly Phe Ala Ala Lys Pro Ala Thr Leu Glu Ser Val Phe Met Asp 260 265 270 <210> 350 <211> 27i <212> PRT <213> Corynebacterium giutamicum <400> 350 Gly Leu Asn Phe His Val Gin Arg Gly Glu Val Phe Gly Leu Leu Gly 1 5 10 Thr Asn Gly Ala Gly Lys Thr Ser Thr Leu Giu Val Ile Glu Gly Leu 25 Ser Ala Pro Ser Ser Gly Thr Vai Arg Ile Ser Gly Leu Asp Pro Val 40 Ala Asp Arg Ala Ile Leu Arg Pro Giu Leu Gly Ile Met Leu Gin Ser 55 Gly Gly Leu Pro Ser Gin Leu Thr Vai Ala Giu Thr Met Asp Met Trp 70 75 His Gly Thr Cys Thr Tyr Pro Arg Ala Ile Lys Asp Val Leu Ala Asp 90 Val Asp Leu Leu His Arg Giu Asn Val Lys Val Gly Ala Leu Ser Gly 100 105 110 Gly Giu Gin Arg Arg Leu Asp Leu Ala Cys Ala Leu Leu Gly Asp Pro 115 120 125 WO 01/00805 Ser Ile Leu-Phe 130 Arg Arg His Thr 145 Thr Met Met Leu Asp Arg Ile Ala 180 Leu Asp Giu Leu 195 Arg Gly Gly Gin 210 Arg Asp Asn Asn 225 Thr Leu Glu Ile Gly Phe Ala Ala 260 Leu T rp Thr 165 Ile Val1 Val1 His Leu 245 Lys Asp Gin 150 Thr Met Ala Glu Val1 230 Thr Pro Giu 135 Leu His Asn Arg Leu 215 Arg Trp Ala Thr Asp Giu 170 Glu Ser Le u Thr Glu 250 Giu Gly Leu 155 Giu Ile Ile Ser Thr 235 Thr Ser Asp Gin Giu Vali Ser 205 Ala Leu Ile Phe Pro Arg Ph e Giu 190 Phe Glu Gin Ala Met 270 Giu Gly Leu 175 Gly Val Ile Gin Le u 255 Asp PCTIBOO/00926 Ser Val1 160 Cys Thr Leu Ile His 240 Giu <210> 351 <211> 999 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(976) <223> RXA02749 <400> 351 caacciagac ttcggtaaga agtaacttg ctitaqttgg aqcgatgcgc cgatiacttg titigctac aaatttaact aaa Lys ggt Gly gag Giu gati Asp ga c Asp ttt Phe gca Ala acc Thr ci c Leu ga t Asp g ga Gly t ci Ser ga c Asp at Ile ica Ser ca g Gin icggcgcaic aciiicccia aig ica ccc aic cia Met Ser Pro Ile Leu 1 acc git gcg git gac Thr Val Ala Val Asp tit qcc itt cia ggg Phe Ala Phe Leu Gly igc cig ait ggc ii Cys Leu Ile Gly Ile ggi ggc caa gta gat Gly Giy Gin Val Asp 115 163 211 259 307 WO 01/00805 WO 0100805PCTIBOOIOO926 tct Ser ctg Leu gtg ValI ggg Gly cgc Arg ctt Leu 150 tgg Trp ttg Le u atc Ile cg C Arg act Thr 230 ga c Asp gca Ala atg Met gaa Glu a gt Se r qqq Gly ttt Phe acc Th r 135 gat Asp ga c Asp acc Thr att Ilie gcc Ala 215 cat His cga Arg a ca Thr gac Asp aaa Lys gcc Ala aag Lys gcg Al a 120 ga c Asp gaa Giu acc Th r act Thr ga c Asp 200 cg t Arg t cg Ser ctg Leu qa a Giu ga t Asp ctt Le u aaa Lys ca g Gin 105 ga c Asp atc Ile ccc Pro atc Ilie cac His 185 cgt Ar g tac T yr gg t Gi y Arg ct a Leu 265 gt a Val1 gga gtg gtg Gly Val Val 75 gaa aac ttg Glu Asn Leu cgg gtt gca Arg Vai Ala cgc cga tac Arg Arg Tyr gca cga gct Ala Arg Ala 140 aca gca ggc Thr Ala Gly 155 aat tcc ctg Asn Ser Leu 170 tac atg gaa Tyr Met Glu ggc aaa gag Gly Lys Glu acc aca aca Thr Thr Thr 220 aaa gag ttg Lys Glu Leu 235 atc aag ttg Ile Lys Leu 250 gat ggq gtt Asp Gly Val ttt cta gca Phe Leu Ala ttt P he ga a Glu cag Gln gga Gly 125 tta Le u ctc Leu cgt Arg gaa Glu gtc Val1 205 ga a Glu gcc Al a gaa Glu ctc Le u gt t Val1 285 caa Gln cg c Arg at t Ile ttg Leu cac His ccc Pro ga t Asp 175 gaa Glu tca Ser act Thr ttg Leu ggg Gly 255 gta Val1 gct Ala tcc Ser gga Gly gag Giu tcg Ser agc Se r aga Arg 160 gtg Val ctg Le u gga Gly ctt Le u agc Ser 240 ctc Le u gag Glu ga a Glu gtc Val1 ca g Gin caa Gln ggc Giy c ca Pro 145 tca Ser ggc Gly gct Ala a cc Th r a ga Arg 225 cca Pro gaa Glu atc Ile Arg tta Leu c tg Leu atc Ile ggt Gly 130 gac Asp cga Arg ctc Le u ga t Asp ccg Pro 210 aca Th r ga a Gi u gct Ala cgc Arg aaa Lys 290 ga c Asp tac T yr ggg Gly 115 ga a Glu att Ile cgc Arg act Thr t ca Ser 195 atg Met aac Asn atc Ile g cg Ala ca c His 275 cga Arg 280 tgattacagtI <210> 352 :ctgacacgc aga WO 01/00805 <211> 292 <212> PRT (213> Corynebacterium giutamicum PCTIBOOIOO926 <400> 352 Met 1 Th r Phe Cys Gly Val1 Gin Gin Giy Pro 145 Se r Giy Ala Th r Arg 225 Pro Giu Ile Ser Pro Ile Leu Lys Val Arq Asp Val1 Ala Leu Giy Leu Leu Ile Giy 130 Asp Arg Le u Asp Pro 210 Thr Giu Ala Arg Ala Phe Ile Gin Asp Tyr Gly 115 Giu Ile Arg Thr Ser 195 Met Asn Ile Ala His Val Le u Gly Val1 Pro Pro 100 Met Lys Leu Gin Val1 180 Val1 Gi u Asp Asp Arg 260 Giy 5 Asp Gly Ile Asp Leu Gly Giu Arq Ph e Vai 165 Phe Le u Le u Pro Gly 245 Leu Gly Giu Asp Ser 70 Leu Val Gly Arg Leu 150 Trp Leu Ile Arq Thr 230 Asp Ala Leu Asn Gin 55 Giu Ser Gly Phe Thr 135 Asp Asp Thr Ile Ala 215 His A rgc Th r Asn Giy Ala Lys Aia Lys Ala 120 Asp Giu Thr Th r Asp 200 Arg Ser Leu Glu Asp 280 Phe 25 Ala Th r Le u Lys Gin 105 Asp Ile Pro Ile His 185 Arg Tyr Gly Arg Leu 265 Leu 10 Asp Giy Ser Gly Giu 90 Arg Arg Ala Thr As n 170 Tyr Giy Th r Lys Ile 250 Asp Val1 Val1 Ly s Gly Val1 75 Asn Val1 Arg Arg Ala 155 Ser Met Ly s Thr Giu 235 Lys Gly Lys Ser Thr Glu Val1 Leu Ala Tyr Ala 140 Giy Leu Giu Giu Thr 220 Leu Leu Val T yr Gly Thr Giu Gin Thr Leu 110 Vai Le u Asp As n Thr 190 Ala Le u His Asn Asp 270 Gi y Giu Ile Leu Gin Arg Ile Le u His Pro Asp 175 Giu Ser Thr Leu Gly 255 Val1 Asp Ile S er Gin S er Gly Giu Ser Se r Arg 160 Val1 Leu Gly Leu Ser 240 Leu Glu Ser Met Asp Val Phe Leu Ala Val Thr Ala Glu 275 Arq Lys Arg Ser 290 wo 01/00805 WO 0100805PCT/BOOIOO926 <210> 353 <211> 1920 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <222> (101)..(1897) <223> RXA02224 <400> 353 gcttcgtcga ggccggaaaa ccgtcgtcat tacgtcgaac ccgacgtggc acggcgtcgc aaagcagatg caatctgatt tttcggaagg ggtgaagtag atg gcg cag cat gag Met Ala Gin His Glu cgc Ar g gag Giu ttc Phe aac Asn ctg Leu gg C Gly gt g Val1 gcg Ala ggc Gly gtg Val 150 att Ile gcg Ala aaa Lys gt t Val ttg Le u gg t Gly gga Gly aac As n tct Ser 120 gga Gly atc Ile ccc Pro ga t Asp a cg Thr att Ile Gly tca Ser att Ile acc Th r 105 tcg Ser aac As n gt c Val1 agc Ser cag Gin tcg Ser gtc Val1 gt t Val a cc Thr gaa Giu Cgt Arg cqt Arg a gc Ser 140 gta Val ttc Phe gag Giu tgg T rp gtc ValI cag Gin ctt Leu ctg Leu ctg Leu gt c Val 130 atc Ile atc Ile ca c His t cg Ser tat Tyr ggc Gly t cg Ser gg t Giy ctg Leu cgg Arg 115 atg Met gat Asp acc Thr tgg Trp t gg T rp gtg Vali acg Thr ttg Leu ttg Leu caa Gin 100 aaa Lys gaa Giu aac Asn att Ile acc Thr 115 163 211 259 307 355 403 451 499 547 595 643 WO 01/00805 WO 0100805PCTLBOO/00926 qtg Val1 aaa Lys gag Glu aca Th r 230 ca a Gin ttc Ph e att Ile agc Ser att Ile 310 tct Ser a cc Thr act Thr gag Giu ggt Gly 390 atc Ile gcc Ala gcg Ala 215 ctg Leu g cg Ala act Thr g9C Gly att Ile 295 cac His act Th r ga a Glu gtg ValI ga t Asp 375 act Thr ctg Le u gtg ValI 200 cgt Arg cgt Arg t cg Se r cgt Arg qtg Val1 280 gga Giy gt g Vali tct Ser cag Gin ca g Gin 360 ttc Phe tct Ser ttc Phe 185 cga Arg cg a Ar g gt g Val tgg Trp ct g Le u 265 ttt Ph e gcg Al a ttc Ph e ctt Le u ctg Leu 345 gac Asp aat As n gg t Gly atc gca gtg Ile Ala Vai gct att cct Ala Ile Pro aac aat ctg.

Asn Asn Leu 220 ctc aag ctc Leu Lys Leu 235 act gcg gtg Thr Aia Vai 250 ctc gcc ctt Leu Ala Leu ggg ctc agt Gly Leu Ser gca acg gca Ala Thr Ala 300 aac qtg ctg Asn Val Leu 315 ggt cgc gcg Gly Arg Ala 330 tct gag tct Ser Giu Ser gtq acg ttt Val Thr Phe ctg gtc ttg Leu Val Leu 380 gcg qga aaa Ala Giy Lys 395 att gtg gtg Ile Val Vai 190 tcg gca aca Ser Ala Thr 205 ctc ctc gat Leu Leu Asp ggt qcg tgg Giy Aia Trp caa gca aca Gin Ala Thr 255 qgt tcq att Gly Ser Ile 270 gcg ttt tgg Ala Phe Trp 285 gca gtt ttc Ala Val Phe ttc ttc gca Phe Phe Ala gtg tcc ctt Vai Ser Leu 335 qcc gat tgc Ala Asp Cys 350 aaa tat ccc Lys Tyr Pro 365 gaa qca gga Giu Ala Gly tcc acg ctc Ser Thr Leu gcc gtt ttg Ala Val Leu 415 ctg att ccc agc Leu aat As n a cg Th r ggt Gly 240 g ct Ala gct Ala qt t Val1 gtg Val1 t cg Se r 320 gcc Ala aca Th r ggc Giy aca Thr gcg Ala 400 gt c Val1 Ile att Ile atc Ile 225 gt g Val1 ga t Asp tat Tyr gcc Ala gt t Val1 305 ga a Glu ca g Gin ga a Glu ggc Giy acc Thr 385 ggc Gly ggg Gly Pro Ser 195 gtg tcc Vai Ser 210 cgt ggc Arq Gly cag cgg Gin Arg cgc gcg Arg Ala ggc ctg Gly Leu 275 cag gat Gin Asp 290 gtg cgc Val Arg att cag Ile Gin atq qct Met Ala cca ccc Pro Pro 355 gtg gcc Val Ala 370 aca gcg Thr Ala gtc att Val Ile ggc atc Gly Ile ggt Gi y a gt Ser att Ile atg Met c cg Pro 260 ctg Le u gcq Ala atg Met agt Ser cqt Arg 340 tcc Ser att Ile ctg Leu gcg Ala aac As n 420 cgg Arq acg Th r ga a Glu cgc Arg 245 att Ile ct a Leu atg Met ga a Giu g cg Ala 325 cgc Arg gtg Val1 ttg Le u gt c Val1 ggg Gi y 405 acc Th r 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 ctq cag cgc cct gat tcc ggc Leu Gin Arg Pro Asp Ser Gly 410 WO 01/00805 WO 0100805PCTIBOOIOO926 gcc Ala cag Gin 9CC Ala gtc Val1 470 gga Gly atc Ile gtg Val1 cgc Arg t tg Le u 550 atc Ile tta acc Th r gaa Giu aat As n 455 ggg Gly tta Leu caa Gin ttg Le u atg Met 535 gtt Val1 gtg Val1 ctt gt c ValI gtc Val1 440 gcg Ala ctc Leu gac Asp cag Gin atc Ile 520 ttg Leu gt t Val1 atg Met qct a cc Th r 425 cac His cac His ggg Gi y acc Th r caa Gin 505 atg Met gaa Gi u q cg Al a ga a Glu ttt gac acg tgg act acc cgc cag gtt Asp Thr Trp Thr Thr Arq Gin Val 430 gcg ctg atc agc Ala Leu Ile Ser 435 ctt Leu gcc Ala caa Gin aaa Lys 490 atc Ile ga t Asp aaa Lys cac His caa Gin 570 gag Glu ttc Phe acc Th r atg Met 475 att Ile tct Ser gaa Giu gcc Ala cgc Arg.

555 ggc Gly gyc Giy gca ggc act Ala Giy Thr 445 gac gcg cag Asp Ala Gin 4 60 aca act gct Thr Thr Ala ggc gcc ggc Gly Ala Gly ctt gcc cgc Leu Ala Arg 510 gcc acc agt Ala Thr Ser 525 gcc aca gaa Ala Thr Glu 540 ctt gac caa Leu Asp Gin aca atc acc Thr Ile Thr cqc tac gcg Arg Tyr Ala 590 ctg Leu ctc Leu ttt Phe g ca Ala 495 atc Ile gaa Glu atc Ile gca Al a gaa Glu 575 gca Ala ca c His cga Arg 480 gaa Giu gtg Va I gcc Al a g ca Ala gt t Val1 560 gac Asp gag Glu gca Ala 465 cg t Arg ga a Glu ctc Le u ggc Gly cg a Arg 545 qtc Val 9gc Gly gat Asp 450 gcg Ala ttc Phe C tc Le u cqc Arg a qc Ser 530 aac As n gca Ala act Thr ctt Leu ctc Leu ttt Ph e acc Th r aat As n 515 gat Asp Cgc Arg ga t Asp cac His cgc Arg gag Glu cca Pro cct Pro 500 cca Pro gat Asp acc Th r cgc Arg ca g Gin 580 atg Met t cg Se r tcc Ser 485 ga a Giu cct Pro gCC Al a acc Th r atc Ile 565 gaa Glu 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1920 Leu Leu Ala Phe 585 cag ctg tat caa cga tgg agt Gin Leu Tyr Gin Arg Trp Ser 595 gct caa Ala Gin tagttcaaat ccaccacaaa ctc <210> 354 <211> 599 <212> PRT <213> Corynebacterium giutamicum <400> 354 Met Ala Gin His Glu.Arg Val Ala Asp Ala Leu Gin Pro Ala Ser Leu 1 5 10 Ala Glu Ser Trp Arg Giu Leu Lys Thr Met Pro Ser Gly Pro Lys Ala 25 WO 01/00805 WO 0100805PCTIBOO/00926 T rp Met Gly Le u Ala Asp Asp Asp 145 ValI Ile Ile Ile Ile 225 Val2 Asp Tyr Ala Val1 305 Glu Gin Tyr Gly Ser Gly Leu Arg 115 Met Asp Thr Trp Ser 195 Ser Gly Arg Ala Le u 275 Asp Arg Gin Aila Val1 Th r Leu Leu Gin 100 Lys Giu As n Ile Th r 180 Gly Se r Ile Met Pro 260 Leu Aia Met Ser Arq 340 S er Ser Pro Val2 Leu Aia Leu Thr Leu 165 Ph e Arg Thr Giu Arg 245 Ile Leu Met Giu Ala 325 Arg Ph e As n Leu 70 Gly Val1 Ala Giy Val 150 Ile Val1 Lys Giu Th r 230 Gin Phe Ile Ser Ile 310 Se r Thr Val Leu Ile Ala Ile Le u Th r 135 Ar g Le u Ile Ala Al a 215 Le u Aia Thr Gly Ile 295 His Thr Glu Val1 Ser Ser 75 Ala Ala Leu Ile Met 155 Le u Ala Ile Asn Lys 235 Ala Aia Le u Th r Vai 315 Arg Giu ValI Leu Met Ala Leu 1 Pro Leu Val Leu Val 190 Al a Le u Aia Aila Ser 270 Ph e Vali Phe Se r Asp 350 Val1 Ile Ile Giy Ser ValI Th r Arg Met 175 ValI Thr Asp Trp Thr 255 Ile Trp Phe Ala Leu 335 Cys Ala Asn Trp Arg Vali Pro Gin Le u 160 Th r Le u Asn Thr Giy 240 Ala Ala Val Vali Ser 320 Aia Th r WO 01/00805 WO 0100805PCT[IBOOIOO926 Glu Pro Pro Ser Val Thr Val Gin Asp Val Thr Phe Lys Tyr Pro Gly Gly Thr 385 Gly Gly Val1 Glu Ala 465 Arg Glu Leu Gly Arg 545 ValI Gly Tyr Ile Leu Ala As n 420 Ile Arg Glu Pro Pro 500 Pro Asp Thr Arq Gin 580 Trp 360 365 Leu Val1 Gly 405 Thr Ser Met Ser Ser 485 Glu Pro Ala Th r Ile 565 Giu Se r Asn Gly Pro Thr 425 His His Gly Thr Gin 505 Met Glu Ala Glu Phe 585 Val Gly 395 Se r Thr Phe Th r Met 475 Ile Ser Glu Ala Arg 555 Gly Gi y Leu 380 Lys Gly Trp Ala Asp 460 Thr Gly Leu Ala Ala 540 Leu Thr Arg Glu Ser Al a Th r Giy 445 Ala Th r Ala Ala Th r 525 Th r Asp Ile Tyr Thr Ala 400 Val Gin Ala His Arg 480 Giu Val1 Al a Ala Val 560 Asp Leu <210> 355 <211> 1653 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1630) <223> RXN01602 <400> 355 tgcaggccac atgcctccca gtgccgtctc tgcacgttga ttttcccctg ccacgactgg WO 01/00805 tcgcagggcg actttctagc acttttaaag gaatttttta PCTIBO/00926 aaa acc cat 115 Lys Thr His att Ile a cg Th r ggt Gly ga a Glu .Att Ile tgt Cys aga Ar g tat Tyr ga a Glu a ga Arg 150 C9g Arg ga a Glu gc c Al a gca Ala tcc Ser gtt Val1 aaa Lys gg t Gly cct Pro ga t Asp gag Glu gcc Al a gct Al a 140 gag Glu gg t Gly gac Asp ggt Gly g cg Ala 220 atg gct Met Ala 1 tca aco Ser Thr tgc gca Cys Ala cga gca Arg Ala cat ggc His Gly tcc gtc Ser Val ctc gaa Leu Glu cac gca His Ala gac gca Asp Ala 130 ggc gcg Gly Ala 145 caa agq Gin Arg att ttg Ile Leu aac tat Asn Tyr gtt tct Val Ser 210 gat att Aso Ile ccg Pro gtg Val1 ctg Leu a cg Thr gga Gly gag Glu ctt Leu 115 tgg T rp atc Ile tat T yr ctt Leu ctc Leu 195 cat His ga t Asp WO 01/00805 WO 0100805PCT/1B00/00926 cca Pro 230 cgCc Arc; g ca Ala cac; Gin act Thr cac; Gin 310 cca Pro ga c Asp cat His gct Ala cca Ala 390 ga a Giu aag Ly s ttc Ph e g9C; Gly at g gac Asp caa Gin ca a Gin Arc; cgc Arc; 295 gat Asp ttg L eu ttt Phe tca Ser 9C~g Gly 375 agt Ser tca Se r tgg Trp tca Ser aat Asn 455 cac ggc Gly Arc; act Thr cgtc Val1 280 gca Ala c9cg Ala ct t Leu t cc Ser gtc; Val 360 aaa Lys ggt Gly ctt Leu cgga Gly att Ile 440 cct Pro ttc; cta Leu agt Ser gtg Val 265 aat As n tct Se r ttg Leu ct g Le u ga c Asp 345 gt a Val1 tca Se r tcc; Se r gt c Vai ctt Le u 425 Gi y cgaa Gi u cgtt cca Pro gcg Ala 250 ca a Gin t ct Ser cgg Arc; cgat Asp cct Pro 330 ctt Leu tca Ser acc; Th r cgtt Val .gcc Gly 410 tta Leu caa Gin ctc; Leu tcC cgg Arc; 235 ctt Leu cac; Gin tcc; Ser cgct Ala ac Se r 315 acc Th r ttt Phe gcgt Gly ctc Le u cgca Ala 395 gag Giu acgt Ser cgcc Arc; ttg Leu g;ca atc Ile ctt Le u caa Gin Trp ccc Pro 300 aaa Lys ttg Leu cgta Val gac Asp atc Ile 380 aac Asn Val1 cgtt Val aga Arc; ctt Le u 460 ctt t ct Ser tat Tyr cac His 275 c9ca Gly aac; Lys cgct Aia ccc Pro cca Pro 355 cgac Asp ttc; Leu ctc Le u cac His cta Leu 435 tcc; Ser cat His acc tat Tyr gcc Ala 260 gca Ala aaa Ly s ccga Arc; ccc; Pro atc; Met 340 9Cgc Gly aac As n act Th r 9Cc Gly cqca Ala 420 cac; Gin tta Leu ctc; Leu cg 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 WO 01/00805 PCT/IBOO/00926 Met His Leu Val Ser Ala Leu Thr Giu Trp Leu Asp Thr Thr Ala Ala 470 475 480 485 gca gtg atc atq gta acg cat gat cga cag cta ctc cgc gat acg gct 1603 Ala Val Ile Met Val Thr His Asp Arg Gin Leu Leu Arq Asp Thr Ala 490 495 500 cat tgg agg cac atc gag ttg aaa tct taagaattcg caagqgcttt 1650 His Trp Arg His Ilie Giu Leu Lys Ser 505 510 cac 1653 <210> 356 <211> 510 <212> PRT <213> Corynebacterium qiutamicum <400> 356 Met Ala Lys Thr His Ilie Arg Leu Gin Asp Leu Ser Leu Ser Tyr Thr 1 5 10 Ser Thr Pro Leu Ilie Thr Lys Leu Asn Ilie Thr Val Ser Ser Gly Gin 25 Cys Ala Val Ilie Vai Gly Glu Asn Gly Arg Gly Lys Thr Thr Leu Leu 40 Arg Ala Leu Ala Arg Glu Phe Pro Pro Ser Ala Gly Glu Ile Leu Thr 55 His Giy Thr Val Ala Ilie Ala His Gin His Met Pro Ala Gly Asp Leu 70 75 Ser Val Gly Giu Ile Cys Asp Giu Ala Ile Arq Asp Ser Lys Asn Ala 90 Leu Glu Giu Leu Glu Arg Ala Gly Ala Leu Leu Glu Thr Asn Thr Ala 100 105 110 His Ala Leu Asp Gly Tyr Gin Gin Ala Leu Asp Ala Ala Giu Val Leu 115 120 125 Asp Ala Trp Asn Ala Glu His Arq Leu Giu Lys Ala Leu Arg Ser Phe 130 135 140 Gly Ala Ilie Thr Asp Arg Ser Arg Ala Leu Ser Glu Leu Ser Ilie Gly 145 150 155 160 Gin Arq Tyr Arg Val Arg Leu Ala Cys Leu Ile Gly Gly Asp Ala Asp 165 170 175 Ilie Leu Leu Leu Asp Giu Pro Thr Asn His Leu Asp Arg Gly Ala Leu 180 185 190 Asn Tyr Leu Thr Giu Ala Ile Thr Ser His Lys Gly Val Val Leu Val 195 200 205 Val Ser His Asp Gin Ala Leu Ile Lys Asp Val Ala Asp Phe Ile Ile 210 215 220 WO 01/00805 Asp Ilie Asp SE 225 Phe Asp Ser TN Gin Asp Tyr Al 2E Leu Giu His Al 275 Giy Thr Gly L 290 Ala Leu Lys Ar 305 Pro Pro Ala Pr Asp Lys Pro Me 34 Arg Leu Pro Gi 355 Thr Giy Asp As 370 Giy Val Leu Th -385 Gly Val Leu Gi Arg Asp His Ai 42 Phe Ala Leu Gi 435 Ala Met Ser Le 450 Ser Asn His Le 465 Asp Thr Thr Al Pro 230 Arg Aila Gin Th r Gin 310 Pro Asp His Ala Ala 390 Giu Lys Phe Gly Met 470 Ala Asp Gin Gin Arg Arg 295 Asp Le u Ph e Ser Giy 375 Ser Ser Trp Ser Asn 455 His Val Giy Arg Th r ValI 280 Ala Ala Le u Ser Vali 360 Lys Gly Leu Gly Ile 440 Pro L eu Ile Leu Ser Val 265 Asn Ser Leu Leu Asp 345 Val Ser Ser Vai Leu 425 Gly Glu Val Met Giu T rp 255 Glu Pro Val1 Asp Arg 335 Arg Val Le u Arq Ile 415 S er Asp Glu Trp Gin 495 PCTIBOOIOO926 Gly 240 Arg Asp Lys Gin Vali 320 Pro Leu Ile Se r Thr 400 Al a Arq Leu Pro Le u 480 Leu Leu Arg Asp .Thr Aia His Trp Arg His Ile Giu Leu Lys Ser <210> 357 <211> 879 <212> DNA <213> Corynebacterium glutamicum WO 01/00805 <220> <221> CDS <222> (101)..(856) <223> RXN02515 <400> 357 gtggctaagc acagttactt ggccaagctg qgcggcagaa PCT/iBOOIOO926 aaaccggccc agctaatact tcagtttaaa attcgcttca accctgaaag attgtgacag atg agc act ctt gaa Met Ser Thr Leu Glu atc Ile cct Pro atc Ile t a c Tyr ctc Leu cg c Arg gt t Val gag Giu ga a Glu gg t Gly 150 ct g Leu ga c Asp ca g cgt Arg a ag L ys cac His a cc Th r ctc Leu gct Al a tcC Ser gct Ala gct Ala 135 ttc Ph e ct g Leu gtg Val gag aac Asn gaa Glu g cc Al a ctt Leu ga c Asp gg t Gly gtt Val1 cct Pro 120 c ig Leu tcc Ser aag L ys ga t Asp acc ctg Le u atc Ile atc Ile 9gt Gly ggc Gly ctc Le u gct Al a 105 aag Lys g ca Ala gg t GI y cca Pro gca Ala 185 gaa cac His ctc Leu a tg Met gga Gly gag Glu ttc Phe aac Asn ctt Leu att I le gg c Gly aag Lys 170 ctg Leu ggt gca Ala aag Lys ggc Gly ca c His aac As n 75 ctg Leu ttc Ph e cgc Arg ga c Asp gag Glu 155 ttc Ph e cg c Arg ggc ccg Pro ct c Leu tcC Ser gag Glu atg Met tat Tyr gca Al a aag Lys tcc Ser cac His ga t Asp 175 gag Glu atc tcc Se r acc Thr gqc Gly gta Val ga a Glu cca Pro gcg Ala ga a Glu aac As n gag Glu 160 gag Glu ggc Gly acc 1 ga t Asp atc Ile aag Lys acc Th r gtt Val1 act Th r acc Thr gtc ValI cgc Arg 145 gt t Val1 a cc Thr atc Ile cac gag Glu aac Asn t cc Ser gca Ala gat Asp gaa Glu gc-a Ala cgc Arg 130 tca Ser ctg Leu ga c Asp aa c Asn tac 115 163 211 259 307 355 403 451 499 547 595 643 691 739 WO 01/00805 Gin G1u Thr G 200 Ctc aac tac g Leu Asn Tyr V 215 att gtg acc a Ile Val Thr T 230 ggc tac gac c Gly Tyr Asp G Gly cc t Pro ggc Gly 235 atc Ile Ile ga c Asp 220 gct Al a aag Lys PCTIBOO/00926 Leu Met Ile Thr His Tyr Lys Arg Ile 205 210 ttc att cac gtt ttc gcg aat ggc cag 787 Phe Ile His Val Phe Ala Asn Gly Gin 225 gag ctt gct gac aag ctc gag gct gac 835 Giu Leu Ala Asp Lys Leu Glu Ala Asp 240 245 taacatgtcc gatttcctca atg 879 <210> 358 <211> 252 <212> PRT <213> Corynebacterium giutamicum <400> 358 Met Ser Thr Leu Glu Ile Arg Asn Leu 1 Asp Ile Lys Thr Val1 Th r Th r Val Arg 145 Vai Th r Giu Asn Ser Ala Asp Giu Ala Arg 130 Ser Leu Asp Al a Gly Le u Glu Arg Pro 100 Ar g Al a As n Leu Giy 180 5 Giu Glu Ala ValI Ala Giy Gly Gin Glu Asp 165 Leu Pro Ile Tyr Leu 70 Arg Val1 Giu Giu Gly 150 Leu Asp His Leu Met Giy Giu Phe As n Leu Ile Gi y Lys 170 Le u Ala Lys Giy His Asn 75 Le u Ph e Arg Asp Glu 155 Phe Arg Gin Gly Pro Pro Ile Ala Leu Glu Pro 140 Lys Ala Ile Vali Val1 As n Arg Le u Met Arg Trp 125 Giu Lys Ile Val Ser Thr Giy Val1 Giu Pro Ala Giu Asn Glu 160 Glu Gly Ile Asn Ser Tyr Lys Gin Giu Thr Giu Giy Gly Ile Leu Met Ile Thr WO 01/00805 PCTIBOO/00926 His Tyr Lys Arg Ile Leu Asn Tyr Val Lys Pro Asp Phe Ile His Val 210 215 220 Phe Ala Asn Gly Gin Ile Val Thr Thr Gly Giy Ala Giu Leu Ala Asp 225 230 235 240 Lys Leu Giu Ala Asp Giy Tyr Asp Gin Phe Ile Lys 245 250 <210> 359 <211> 1386 <212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <222> (101)..(1363) <223> RXN00525 <400> 359 ccatcgtgtt tattactcac aaccctgaqc ttgctgatga atctgatcgq gtggtcacca tggttgacgg gcgcatcatt qggtctgagg tgaaacactc atg aqc ctt gca gaa 115 Met Ser Leu Ala Giu 1 tca att ctt ttg qcq ctc acc agc ctg aga agc aac aaq atg cgt gca 163 Ser Ilie Leu Leu Ala Leu Thr Ser Leu Arg Ser Asn Lys Met Arg Ala 15 ttg ttg acg ctg tta gga gtc atc att ggt atc gca tca gtc atc gga 211 Leu Leu Thr Leu Leu Gly Vai Ile Ile Gly Ile Ala Ser Val Ile Giy .30 att ttg acc att qgt aaa gcc cig cag gat caa act ttg aat agt ttq 259 Ile Leu Thr Ile Gly Lys Ala Leu Gin Asp Gin Thr Leu Asn Ser Leu 45 gaa agc ttg ggc gcg aat gat ctg tcg gcg cag gtg gag gaa cgc ccc 307 Giu Ser Leu Gly Ala Asn Asp Leu Ser Ala Gin Val Giu Giu Arg Pro 60 gac gaa gat tcc ccc gaa ccc gat atg ttc gct ttt tct ggg gct gca 355 Asp Glu Asp Ser Pro Giu Pro Asp Met Phe Ala Phe Ser Gly Ala Ala 75 80 aac tct agt ggc aat ctg att ccg gaa gaa aca gtt gat acg ctg cgc 403 Asn Ser Ser Giy Asn Leu Ile Pro Giu Giu Thr Val Asp Thr Leu Arg 95 100 gat cgt ttc gca ggc agc atc acq qga atc agc gtt ggc gga atg ggt 451 Asp Arg Phe Aia Giy Ser Ile Thr Gly Ile Ser Vai Gly Gly Met Giy 105 110 115 acg caa ggc act ctc atc ggc gac acc gca gat ctt aaa tcc gat ctc 499 Thr Gin Giy Thr Leu Ile Gly Asp Thr Ala Asp Leu Lys Ser Asp Leu 120 125 130 ctc ggc gtc aac gag gat tat atg tgg atg aat ggc gtc gaa atg aac 547 Leu Gly Vai Asn Giu Asp Tyr Met Trp Met Asn Giy Vai Giu Met Asn WO 01/00805 WO 0100805PCTftBOOIOO926 tac Tyr 150 9 cq Ala aac As n aca Th r ct t Le u gcc Ala 230 c9 t Arg acc Th r a tg Met gca Ala ggt Gly 310 a cc T hr att Ile Gly agc Ser ggc Gly gt c Val1 ctc Leu ttt Ph e gtg Val1 215 aat Asn 9 ca Ala tac Tyr ttg Leu atg Met 295 ggc Gi y cgc Arg cg C Arg atc Ile gc t Ala 375 C gc Arg atc Ile gct Ala t tg Leu 200 gga Gly ga c Asp gct Ala ttc Phe gac Asp 280 agt Ser atc Ile gaa Giu c tg Leu ctc Leu 360 att Ile gcc Ala 9CC Al a ct g Leu 185 cgg Arg agc Se r atc Ile cag Gin ga C Asp 265 ttc Phe ttg Le u 9ga Gly atc Ile caa Gin 345 ggc Giy Gi y atc Ile cca Pro 170 ggg Gly gtt Vai aa t Asn a cc Thr qgc Giy 250 gcg Ala cgt Arg qgt Giy gtg Val1 ggt Gly 330 ttc Phe gtg Val1 tac Tyr a cg Thr 155 gac Asp tcc Ser atc Ile cca Pro cac His 235 gta Vai ctg Leu aaa Ly s atc Ile atg Met 315 gt c Val1 gt c ValI ctt Leu att Ile ca a Gin acc Thr ga a Giu gg t Giy acc Th r 220 a cg Th r ga c Asp tac Tyr ca g Gin t ca Ser 300 aac As n cga Arg gtt Val t tg Le u tcc Ser 380 ga c Asp ttt Phe gta Vali gtg ValI 205 gtc Val1 ga a Glu ca 9 Gin gcc Al a atc Ile 285 gcc Al a att Ile aaa Ly s gaa Giu gg C Gly 365 ttg Leu gcc gct cag Ala Ala Gin 160 ctt ttc gac Leu Phe Asp gaa ctc aac Giu Leu Asn qaa gcc gca Giu Ala Ala 210 tac acc cca Tyr Thr Pro 225 ttg aac acq Leu Asn Thr 240 ctt aag ggt Leu Lys Gly gac tcg cac Asp Ser His ttc aac acc Phe Asn Thr 290 gga att tcc Gly Ile Ser 305 gtg tct gtc Val Ser Val 320 ggc gct cgt Gly Ala Arg atc att tgt Ile Ile Cys ttg gga ttg Leu Gly Leu 370 Ctg agt gga Leu Ser Giy 385 cgc Arg g ca Aia ggt Gly 195 gca Ala tat Tyr tta Le u t ca Se r cac His 275 att Ile ttg Leu acc Th r cga Arg ttc Phe 355 atc Ile ccc Pro aac As n 180 caa Gin ggt Gly a cg Thr agt Ser ctg Leu 260 gt t Val1 ctc Leu ctt Leu gag Giu cg t Arg 340 atc Ile a tg Met gtt Val1 165 ccc Pro gag Giu gg a Gly git g ValI atc Ile 245 caa Gin gcc Al a 99 C Gly gt C Val1 cga Arg 325 gac Asp qgt Gly t CC Ser 595 643 691 739 787 835 883 931- 979 1027 1075 1123 1171 1219 1267 atc gtg atc Ile Val Ile WO 01/00805 PCT/IB00/00926 gcc ttg gta ttt tcc atg gct atc ggc ctg ttt ttc ggc tac tac ccc 1315 Ala Leu Val Phe Ser Met Ala Ile Gly Leu Phe Phe Gly Tyr Tyr Pro 390 395 400 405 gcc aac aag gca gca aag ctc gat cca att gac gcc ttg cgt tat gag 1363 Ala Asn Lys Ala Ala Lys Leu Asp Pro Ile Asp Ala Leu Arg Tyr Glu 410 415 420 taaaagcctc gtttttaagg tag 1386 <210> 360 <211> 421 <212> PRT <213> Corynebacterium glutamicum <400> 360 Met Ser Leu Ala Glu Ser Ile Leu Leu Ala Leu Thr Ser Leu Arg Ser 1 5 10 Asn Lys Met Arg Ala Leu Leu Thr Leu Leu Gly Val Ile Ile Gly Ile 25 Ala Ser Val Ile Gly Ile Leu Thr Ile Gly Lys Ala Leu Gin Asp Gin 40 Thr Leu Asn Ser Leu Glu Ser Leu Gly Ala Asn Asp Leu Ser Ala Gin 55 Val Glu Glu Arg Pro Asp Glu Asp Ser Pro Glu Pro Asp Met Phe Ala 70 75 Phe Ser Gly Ala Ala Asn Ser Ser Gly Asn Leu Ile Pro Glu Glu Thr 90 Val Asp Thr Leu Arg Asp Arg Phe Ala Gly Ser Ile Thr Gly Ile Ser 100 105 110 Val Gly Gly Met Gly Thr Gin Gly Thr Leu Ile Gly Asp Thr Ala Asp 115 120 125 Leu Lys Ser Asp Leu Leu Gly Val Asn Glu Asp Tyr Met Trp Met Asn 130 135 140 Gly Val Glu Met Asn Tyr Gly Arg Ala Ile Thr Gin Asp Asp Val Ala 145 150 155 160 Ala Gin Arg Pro Val Ala Val Ile Ala Pro Asp Thr Phe Asn Thr Leu 165 170 175 Phe Asp Ala Asn Pro Asn Leu Ala Leu Gly Ser Glu Val Ala Phe Glu 180 185 190 Leu Asn Gly Gin Glu Thr Phe Leu Arg Val Ile Gly Val Tyr Lys Glu 195 200 205 Ala Ala Ala Gly Gly Leu Val Gly Ser Asn Pro Thr Val His Thr Tyr 210 215 220 Thr Pro Tyr Thr Val Ala Asn Asp Ile Thr His Thr Glu Asp Gly Leu WO 01/00805 WO 0100805PCTIBOOIOO926 225 Asn Lys Se r Asn Ile 305 Ser Ala Ile Giy Ser 385 P he Ala Ser Le u 260 Val1 Leu Le u Giu Arg 340 Ile Met Val1 Tyr Tyr 420 Ile 245 Gin Aia Giy Vai Arg 325 Asp Gly Se r Ile Pro 405 Giu Arg Ala Ala Gin Vai Asp Gin Asp Ser Leu Thr Met Ala Gly 310 Thr Ile Giy Ser Al a 390 Ala Tyr Leu Met 295 Gly Arg Arg Ile Ala 375 Leu As n Ala Ile 285 Ala Ile Lys Giu Gly 365 Leu Ile Asp As n 270 Giu Ile Met Ala Al a 350 Gly Pro Gly Pro 255 Asn Glu Gi y Leu Leu 335 Met Ile Pro Le u Ile 415 Asp Phe Gly Val1 320 Gly Ile Leu Le u Phe 400 Asp <210> 361 <211> 1815 <212> DNA <213> Corynebacterium giut-amicun <220> <221> CDS <222> (1792) <223> RXN02096 <400> 361 cgcttcgacg acctcaccca cagcgatatc cgcaggaatc tcatcgcggt ttttgatgag ccgttcttgt actcctcctc cataccgcga gaacatctcg atg ggt ttg gat gtc 115 Met Gly Leu Asp Val 1 agt gat gag cag atc gaa cac gca gcc agg ctt gcc cag gct cat gat 163 Ser Asp Giu Gin Ile Giu His Ala Ala Arg Leu Ala Gin Ala His Asp 15 ttt atc gat cgc ctt eca aac aaa tac gag gaa gtc att ggc gaa cgc 211 WO 01/00805 WO 0100805PCTI[BOO/00926 Phe Ile Asp gg c Gly gct Ala

C

Ala ga a Giu ttg Leu gca Ala ctg Leu ga C Asp 150 aca Thr ggc Gly ctg Le u a cc Thr tgg T rp 230 ga t Asp caa Gin ct 9 Leu ttc Ph e att Ile ctg Le u gag Glu ct 9 Leu atg Met 135 aac As n ga a Giu atg Met cca Pro agt Se r 215 ctc Leu cta Leu gca Al a acg Thr Ctg Leu gat Asp cac His CtC Le u gga Giy 120 gct Ala ggt Gly aag Lys t cc Ser gca Ala 200 a cc T hr gt c Val1 gca Ala caa Gin Arg Leu ctt tct Leu Ser gcg cat Ala His 9CC tcc Ala Ser gat gtc Asp Val ggc gat Gly Asp 105 CC9 ttg Pro Leu ctt gat Leu Asp tca cta Ser Leu cag tac Gin Tyr 170 atg cca Met Pro 185 gca acg Ala Thr tcC ggt Ser Gly gtc gcg Vai Ala ttt cca Phe Pro 250 agc acc Ser Thr Pro Asn Lys Tyr Giu Giu Val Ile Giy Glu Arg 30 gg t Gi y ccc Pro act Thr 75 acc Th r C99 Arg a qt Ser ttc Phe

CCC

Pro 155 aag Ly s gca Al a ga a Giu ttc Phe gtC Val 235 a ca Thr tcc Ser 99 t Gly aaa Lys 60 gag Giu att Ile gt t Val1 gag Giu cag Gin 140 agc Ser att Ile acc Thr gaa Giu aaa Lys5 220 atc Ile ctg Leu a cg Th r caa Gin 45 gt 9 Vai ga c Asp ttg Leu 99 t Giy atg Met 125 gat Asp caa Gin ct t Leu C Ct Pro aca Thr 205 ttg Leu 9 C9 Ala atg Met ttg Leu cgc Arg t tg Leu cgc Arg atc Ile ctg Le u 110 cgt Ar g tct Se r gag Giu 9 c9 Ala gag Giu 190 cga Arg ctc Leu t tg Le u CgC Arg tgg Trp ca a Gin gt 9 Vali att Ile atc Ile gtc Val1 ga t Asp ca c His caa Gin cct Pro 175 ctg Le u gt t Vali agt Ser t tg Leu gca Aia 255 tgg Trp cgC Arg ctt Le u ttC Phe gcg Ala ga a Glu cac His ga t Asp ttg Leu 160

C

Ala ctc Le u gat Asp tta Leu ct 9 Leu 240 gcc Aia atc Ile atc Ile gat Asp cag Gin cac His ga t Asp gct Ala CCg Pro 145 tgg T rp cct Pro gCC Ala gcc Ala ttC Phe 225 gtg Val atc Ile 9CC Ala 9CC Al a ga t Asp gcc Ala cgc Arg gga Gly cg t Arg 130 gaa Glu ccg Pro gg t Gly cag Gin ggg Gly 210 aag Lys ggC Gly g a c Asp atc Ile 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 WO 01/00805 WO 0100805PCTJIBOO/00926 gta ValI a eg Thr ttt Phe 310 tcC Ser tcC Ser ct C Leu ctc Leu ttc Ph e 390 age Se r geg Ala gaa Giu gee Ala etc Leu 470 ace Th r ec Pro gta ValI gca Ala 295 gtg Val1 Gly ttC Phe atc Ile gtt Val1 375 cga Arg cag Gin.

ca g Gin gca Ala atc Ile 455 gte Val ggc Gly ate Ile 265 gtc ctt Val Leu 280 cge acc Arg Thr cat cta His Leu cgc atc Arg Ile etc caa Leu Gin 345 ggt gtg Gly Val 360 geg ctg Ala Leu ege atc Arg Ile gte aac Val Asn atg eac Met His 425 gag gaa Glu Giu 440 tac ttc Tyr Phe etc ggt Leu Giy gta etc Val Leu caa caa Gin Gin 505 ctg Leu gg t Gly ttg Leu atg Met 330 t ca Ser gte Vai tcc Ser age Ser geg Al a 410 cgc Arg ttC Ph e ccc Pro tte Phe gt g Val1 490 eta Leu tee Ser gaa Giu eg c Arg 315 acg Thr ggt Gly ac Th r gtg Vali tee Ser 395 gt a Val atg Met cga Aig gg e Giy gge Gly 475 gca Al a age Ser t gg Trp egg Arg 300 etg Le u ege Arg et g Le u at g Met gtg Val 380 a gg Arg ttc Ph e gaa Giu ege Arg ett Le u 460 gea Ala tte Ph e eaa Gin gee Ala 285 e tg Le u tee Ser atg Met g eg Ala etc Leu 365 ceg Pro ct g Leu eac His ga e Asp ctg Leu 445 gge Gly ctg Leu gt g Val1 ate Ile 270 g cc Ala ctt Leu atg Met ace Thr caa Gin 350 gee Ala ate Ile tac T yr gag Glu caa Gin 430 eg t Arg 9g Ala caa Gin et g Leu tte Phe 510 gee Ala tac Tyr age Ser ace Th r 335 aca Th r ate Ile ate Ile ace Th r tee Se r 415 gte Val1 gtg Val1 etc Leu gta Val tac Tyr 495 ga c Asp geg Ala gg C Giy tat Tyr 320 ga c Asp gtt Val1 ace Thr ate Ile get Ala 400 ate Ile ttt Ph e aaa Lys tct Ser a eg Thr 480 atq Met tee Ser ate Ile t tg Leu 305 ttC Ph e ate Ile gte ValI gac Asp gt g Val1 385 tea Ser gee Ala gac Asp tee Ser gaa Giu 465 eg c Arg gg e Gly tac Tyr aac Asn 290 egt Arg gaa Giu gac Asp tet Se r gea Al a 370 ctc Le u eg c Arg gg t Gly aat Asn cag Gin 450 ate Ile gge Gly ctg Leu caa Gin 275 aeg att Thr Ile ctg ege Leu Arg ege ace Arg Thr aae etc Asn Leu 340 gtg ggc Val Gly 355 caa eta Gin Leu act etc Thr Leu gag eaa Glu Gin tta ege Leu Arg 420 tat geg Tyr Ala 435 aeg gee Thr Ala gcc eag Ala Gin gae ate Asp Ile atg tte Met Phe 500 caa gee Gin Ala 515 ate Ile tea Ser a tg Met 325 t eg Ser a eg Thr g ca Ala att Ile gee Al a 405 ace Thr ggC Gi y ate Ile gca Ala tee Ser 485 gge Gly gee Ala 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 WO 01/00805 gtc ggc ttc cgt cgc atc acc gaa ctg ctc gca acg cag ccc agc Vai Giy Phe Arg Arg Ile Thr Glu Leu Leu Ala Thr Gin Pro Ser 520 525 530 cag atc tgg gca cca aca ggc acg cta ggc agg ctg cca cgc agc Gin Ile Trp Ala Pro Thr Gly Thr Leu Gly Arg Leu Pro Arg Ser 535 540 545 tat tgc ttg acg acg tca cct tcg gct att cag acg atc cga tcc Tyr Cys Leu Thr Thr Ser Pro Ser Ala Ile Gin Thr Ile Arg Ser 550 555 560 tagacaacgt caccgtccag atc <210> 362 <211> 564 <212> PRT <213> Corynebacteriun giutamicum PCT/IBOO/00926 gtc i699 Vali ctt 1747 Leu 1792 1815 <400> 362 Met Ala Val1 Ile Asp Gin His Asp Al a Pro 145 Trp Pro Ala Gly Leu Asp Vai Ser Asp Giu Gin Gin Ile Ala Asp Al a Arg Gly Arg 130 Giu Pro Gly Gin Phe Giy Ala Ala 70 Glu Leu Ala Leu Asp 150 Th r Gly Leu Ile Le u Phe 55 Ile Leu Glu Leu Met 135 Asn Giu Met Pro Ile Giu Leu Pro Ser Gly His Pro Ser Thr .75 Val Thr Asp Arg Leu Ser Asp Phe Leu Pro 155 Tyr Lys 170 Pro Ala Thr Giu His Asn Gly Ly s Glu Ilie Val1 Giu Gin 140 Ser Ile Thr G iu Ala Lys Gin Val1 Asp Leu Gly Met 125 Asp Gin Leu Pro Thr 205 Ala Tyr Arg Leu Arg Ile Leu 110 Arg Ser Giu Ala Giu 190 Arg Arg Giu Gin Val1 Ile Ile ValI Asp His Gin Pro 175 Le u Val1 Leu Glu Arg Leu Phe Al a Glu His Asp Leu 160 Ala Leu Asp WO 01/00805 WO 0100805PCTIBOO/00926 Ala Gly 210 Phe Lys 225 Val Gly Ile Asp Ala Ile Ile Asn 290 Leu Arg 305 Phe Giu Ile Asp Val Ser Asp Ala 370 Val Leu 385 Ser Arg Ala Gly Asp Asn Ser Gin 450 Giu Ile 465 Arg Gly Gly Leu Tyr Gin Thr Gin Arg Leu Arg Thr Ser Thr Ser Gly Phe Lys Leu Leu Ser Leu Gin ValI As n Ala 275 Thr Leu Arg Asn Val1 355 Gin Thr Giu Leu Tyr 435 Thr Ala Asp Met Gin 515 Pro Val Ala Gly 260 Gly Ile Arg Thr Leo 340 Gly Leo Leo Gin Arg 420 Ala Ala Gin Ile Phe 500 Ala Ser Arg Ala 245 Val1 Ser Ile Ser Met 325 Se r Thr Ala Ile Ala 405 Thr Gly Ile Ala Ser 485 Gly Ala Val1 T rp 230 Asp Gin Val1 Thr Phe 310 Ser Ser Leu Leu Phe 390 Ser Ala Glu Ala Leo 470 Th r Pro Val Gin 215 Leu Leu Ala Val Al a 295 Val Gly Phe Ile Val1 375 Arg Gin Gin Ala Ile 455 Val1 Gly Ile Gly Ile Val1 Ala Gin Val 280 Arg His Arg Leo Gly 360 Ala A r c Val Met Glu 440 Tyr Leu Val Gin Phe 520 Trp ValI Phe Ser 265 Leo Th r Leu Ile Gin 345 Val1 Leu Ile Asn His 425 Giu Phe Gly Leu Gin 505 Arg Ala Ala Pro 250 Thr Leu Gly Leo Met 330 Ser Val1 Se r Se r Ala 410 A r c Ph e Pro Ph e Val1 490 Leo Arg Pro ValI 235 Thr Se r Ser Glu Arg 315 Thr Gly Thr Val Ser 395 ValI Met Arg Gly Gly 475 Ala Ser Ile 220 Ile Leo Thr T rp Arg 300 Leo Arg Leo Met Val 380 Arg Phe Glu Arg Leo 460 Ala Phe Gin Th r Ala Met Leo Ala 285 Leo S er Met Ala Leo 365 Pro Leo His Asp Leo 445 Gly Leo Val1 Ile Glu 525 Leo Arg T rp 270 Al a Leo Met Thr Gin 350 Ala Ile Tyr Glu Gin 430 Arg Ala Gin Leo Phe 510 Leo Leo Leo 240 Ala Ala 255 Trp Ile Ala Ala Tyr Gly Ser Tyr 320 Thr Asp 335 Thr Val Ile Thr Ile Ile Thr Ala 400 Ser Ile 415 Val Phe Val Lys Leu Ser Val Thr 480 Tyr Met 495 Asp Ser Leu Ala Thr Gly Thr Leo Gly Arg WO 01/00805 PCT11BO0100926 530 535 540 *LuPro Arg Ser Leu Tyr Cys Leu Thr Thr Ser Pro Ser Ala Ile Gin 545 550 555 560 Thr Ilie Arg Ser <210> 363 <211> 1203 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (101)..(1180) <223> RXN00412 <400> 363 cttttgacqa acaccacqtc gcgtacgctt cctcggggcq cttttgtccc ccgacttttg tacgaatcga ggacaccgtc ttaaactatt tgtcttccaq tcc Ser ggc Gly aaa Lys ccc Pro act Thr ttg Leu cg t Arg ttc Phe aca Thr act Th r tct Ser ggt Gly ctt Leu ct g Leu aaa Ly s ca g Gin cca Pro gag C iu a cc Thr atc Ile ctc Leu ggc G ly a gt S er act Thr gag Giu ttc Phe acc Thr ggc Giy atc Ile 75 acc Thr aat As n gcg Ala gaa Glu cgc Arg gcg Ala atc Ile 60 aat Asn ga c Asp atc Ile gct Ala tac T yr gg c Gly ctt Le u atc Ile Gly atc Ile Gly gga Gly 125 tcc Ser ata Ile ga t Asp gg t Gly ctt Le u gtC Val1 atg Met 110 aat As n gcg Ala 15 acc Th r aat As n tac Tyr gac Asp gga Gly 95 att Ile gtg Val1 gct Ala ca g Gin aaa Lys gtc ValI tct Ser tcc Ser 80 atg Met ttc Phe gag Giu cg c Arg gtq Val1 1 caa Gin gtc Val act Thr 9gc Gly ccc Pro ccc Pro cag Gin tac T yr g tg Val1 145 ccc Pro ttt Phe ctc Leu gcc Ala a cg Thr gag Giu cag Gin ccg Pro 130 caa Gin tca cac acc gcg Ser His Thr Ala agc Se r a gc Ser acc Thr qgc Gly agc Ser tct Ser ttc Phe 115 ctg Leu gaa Glu acc Thr aac Asn gta Val aa g Ly s gg t Gly aa g Lys 100 aac Asn gaa Glu atg Met ca g Gin aat Asn gaa Glu tcc Ser t cg S er ctg Le u ctg Leu gt t Val ctc Leu 115 163 211 259 307 355 403 451 499 547 gcc aag atg qac aag gca gct cgt aaa Ala Lys 135 Met Asp Lys Ala Ala Arg Lys 140 WO 01/00805 gag ttc gtc ggc Glu Phe Val Gly 150 tcg ggc ggc cag Ser Gly Gly Gin aat cca acg ctt Asn Pro Thr Leu 185 gaa acc acc cat Giu Thr Thr His 200 ctg ggc atc acc Leu Giy Ile Thr 215 tcc atc gca gac Ser Ile Ala Asp 230 tac ggc agc gtc Tyr Giy Ser Val caa aag ttc gtg Gin Lys Phe Val 265 tcg gaa gat ctg Ser Glu Asp Leu 280 act gaa acg tcc Thr Giu Thr Ser 295 ggt gct ttt gtc Giy Aia Phe Vai 310 caa tca ttt ggc Gin Ser Phe Giy att gaa gag ttc Ile Giu Glu Phe 345 ggc Gly 155 cag Gin ctt Leu gt t Vai gtt ValI gtt Val1 235 gag Giu a cc Thr agc S er ttC Phe atc Ile 315 atg Met caa ga c Asp cgc Arg gcc Ala ctg Le u gtg Val1 220 gct Aia gt g Vai gcg Ala cat His ttt Phe 300 gtt Vai act Th r acc aaa Lys att Ile 175 gcc Ala ctg Leu ca c His gaa Giu aa t Asn 255 aac As n cg t Arg acc Tb r Gi y ct c Leu 335 aag aac Asn 160 gcc Ala acc Th r cgc Arg ga a Glu tcc Se r 240 cca Pro acc Tb r ctg Leu gct Al a gt g Val 320 acc Th r acc Th r ccc Pro gca Ala gct Ala gta Val1 210 gaa Giu aaa Lys aca Tb r ga c Asp acc Thr 290 gct Ala acc Tb r aa c As n a cc Th r gag Glu ctg Leu ttg Leu 195 aac Asn gtt Val1 gtt Vai cag Gln caa Gin 275 att Ile gcc Ala ttg Leu acc Thr atc Ile 355 cag Gin gcc Ala 180 gac Asp cgC Arg g tg Val qt g Val1 gt t ValI 260 gtg Vali ga t Asp gaa Giu caa Gin gc t Aia 340 aag Ly s PCTIBOOIOO926 ctg 595 Leu 165 acc 643 Thr cca 691 Pro gaa 739 Giu cgt 787 Arg gaa 835 Glu 245 gct 883 Ala gaa 931 Giu ctg 979 Leu caa 1027 Gin cgc 1075 Arg 325 gcg 1123 Aia gag 1171 Glu Tyr Gin Thr Leu Thr Lys atc acc cga Ile Thr Arg 360 <210> 364 <211> 360 <212> PRT tgaacgagat gatcctcgca gct 1203 WO 01/00805 PCT/EBOO/00926 <213> Corynebacteriun giutanicun <400> 364 Val Ser His Thr Ala Ser Thr Pro Thr Pro Glu Giu Tyr Ser Ala Gin 1 5 10 Gin Pro Ser Thr Gin Gly Thr Arg Val Giu Phe Arg Gly Ile Thr Lys 25 Val Phe Ser Asn Asn Lys Ser Ala Lys Thr Thr Ala Leu Asp Asn Vai 40 Thr Leu Thr Val Glu Pro Gly Glu Vai Ile Gly Ile Ilie Gly Tyr Ser 55 Gly Ala Gly Lys Ser Thr Leu Val Arg Leu Ile Asn Gly Leu Asp Ser 70 75 Pro Thr Ser Giy Ser Leu Leu Leu Asn Gly Thr Asp Ile Vai Gly Met 90 Pro Giu Ser Lys Leu Arg Lys Leu Arg Ser Asn Ile Gly Met Ile Phe 100 105 110 Gin Gin Phe Asn Leu Phe Gin Ser Arg Thr Ala Ala Giy Asn Vai Giu 115 120 125 Tyr Pro Leu Giu Val Aia Lys Met Asp Lys Ala Ala Arg Lys Ala Arg 130 135 140 Vai Gin Giu Met Leu Giu Phe Val Giy Leu Gly Asp Lys Gly Lys Asn 145 150 155 160 Tyr Pro Glu Gin Leu Ser Gly Giy Gin Lys Gin Arg Val Gly Ile Ala 165 170 175 Arg Ala Leu Ala Thr Asn Pro Thr Leu Leu Leu Ala Asp Glu Ala Thr 180 185 190 Ser Ala Leu Asp Pro Giu Thr Thr His Glu Val Leu Giu Leu Leu Arg 195 200 205 Lys Val Asn Arg Giu Leu Giy Ile Thr Ile Val Val Ile Thr His Giu 210 215 220 Met Glu Val Val Arg Ser Ile Ala Asp Lys Vai Ala Vai Met Giu Ser 225 230 235 240 Giy Lys Val Val Glu Tyr Gly Ser Val Tyr Giu Val Phe Ser Asn Pro 245 250 255 Gin Thr Gin Vai Ala Gin Lys Phe Val Ala Thr Ala Leu Arg Asn Thr 260 265 270 Pro Asp Gln Val Giu Ser Giu Asp Leu Leu Ser His Giu Gly Arg Leu 275 280 285 Phe Thr Ilie Asp Leu Thr Giu Thr Ser Gly Phe Phe Ala Ala Thr Ala 290 295 300 Arg Ala Ala Giu Gin Gly Ala Phe Val Asn Ile Val His Gly Gly Val wo 01/00805 WO 0100805PCTIBOO/00926 Thr Thr Leu Gin Arg Gin Ser Phe Gly Lys 325 330 Gly Asn Thr Ala Ala Ile Giu Giu Phe Tyr 340 345 315 Met Thr Val Gin Thr Leu 320 Arg Leu Thr 335 Thr Lys Thr 350 Thr Thr Ile Lys Giu Ile Thr Arg 355 360 <210> <211> <212> <213> 365 798

DNA

Corynebacterium giutamicum <220> <221> CDS <222> (775) <223> RXN00411 <400> 365 catttggcaa aatgactgtt cgactcaccg gcaacaccgc tgcgattgaa gagttctatc aaaccttgac caaqaccacg accatcaagg agatcacccg atg aac gag atg Met Asn Glu Met 1 ctc gca gct gac Leu Ala Ala Asp aac cgg cta gga Asn Arg Leu Gly acc ttc caa aca Thr Phe Gin Thr gcc atc Ala Ile att gac acc Ile Asp Thr ctg ggt ctt Leu Gly Leu ttg atg gtc atc Leu Met Val Ile atc Ile acc atg gtq gtg Thr Met Val Val gct qqc tta Ala Gly Leu gct ggt gga Ala Gly Gly gtc gtc ggc ctg Val Val Gly Leu ctt tac acc acc Leu Tyr Thr Thr atc ttg Ile Leu aag aac aag gtc Lys Asn Lys Val tac acc att ttg Tyr Thr Ile Leu gtg ctg gtg aac Val Leu Val Asn gtt cga ccc atc Val Arg Pro Ile ttc att att ttg Phe Ile Ile Leu gcc gcc atc aag Ala Ala Ile Lys cta acg gtc gcc Leu Thr Val Ala atg ggc acc tcc Met Gly Thr Ser ggc cga gat gcc Gly Arg Asp Ala ggc atc Gly Ile 100 ttc gtc atg Phe Val Met caa aac ttg Gin Asn Leu 120 gtc gca gcg att ttc tct gtg gct cga Val Ala Ala Ile Phe Ser Vai Ala Arg 110 atc gtg gag Ile Val Glu 115 gct cgc tcc Ala Arg Ser gtc tcc att gat Val Ser Ilie Asp ggt gtc atc gag Giy Val Ile Glu gca Ala 130 WO 01/00805 atg ggt gcg tcc ccg atq cgc atc atc Met Gly Ala Ser Pro Met Arg Ile Ile 135 140 gca ctt gga cca ttg qtt ctg ggt tac Ala Leu Gly Pro Leu Val Leu Gly Tyr 150 155 qtc gat atg tcc gca atg gtc ggc tac Val Asp Met Ser Ala Met Val Gly Tyr 170 gac ttc 9CC att qtt tac ggc tac cgc Asp Phe Ala Ile Val Tyr Gly Tyr Arg 185 190 tac gtt gcc gtc ctg gtt atc gtc atc Tyr Val Ala Val Leu Val Ile Val Ilie 200 205 ctg ggc aat tgg ctg tcc aag aag atc Leu Gly Asn Trp Leu Ser Lys Lys Ilie 215 220 gcatagaaaa acc <210> 366 <211> 225 <212> PRT <213> Corynebacterium glutamicum atc att cca Ile Ile Pro ttc atc gcg Phe Ile Ala ggt ggt ctt Giy Gly Leu 180 aac gaa gtt Asn Glu Val 195 gca gcc cag Ala Ala Gin 210 taaacctctt PCTIIBOO/00926 gaa 547 Giu atc 595 Ile 165 ggt 643 Gly atg 691 Met ctt 739 Leu 785 798 <400> 366 Met Asn Giu Met Ile Leu Ala Ala Asp Gin Val1 Arg Val1 Ala Asp Arg Ala 130 Ile Th r Ala Ala Leu Ile Ala Ile 115 Al a Ile Ala Gl1y Gly Val Lys Gly 100 Val1 Arg Pro Asp Gly Leu Val Th r Val As n Gly 135 Leu Thr Leu 40 Lys Arg ValI Met Leu 120 Al a Gly Leu 25 Val Asn Pro Ala Val 105 Val Ser Pro Leu Ile Leu T yr Ile Thr Ile Pro 125 Ile Gly Gly Ile Leu Thr Ile Ser Phe 110 Gly Ile Tyr Pro Thr Tyr Ile Leu Ile Ser Val1 Ala Thr Thr Met Thr Leu Ile Gly ValI Ile Th r Phe WO 01/00805 145 150 155 Leu Phe Ile Ala Ile Val Asp Met Ser Ala Met 165 170 Giy Gly Gly Leu Giy Asp Phe Ala Ile Vai Tyr 180 185 Asp Asn Glu Val Met Tyr Val Ala Val Leu Val 195 200 Gln Ala Ala Gin Leu Leu Gly Asn Trp Leu Ser 210 215 Ar g 225 <210> 367 <211> 852 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <222> (829) <223> RXN02614 <400> 367 tcattgtata cgccaccctc ggtctgctgt ctgaagcgct PCT[IBOOIOO926 160 Val Gly Tyr Ile Gly 175 Gly Tyr Arg Ala Phe 190 Ile Val Ile Ile Val 205 Lys Lys Ile Met Arg 220 gatcagagct tggqaacgtc acaccttccq ctaccgaaac gcataagaaa gttgctcgcc atg act gcc aca ttg Met Thr Ala Thr Leu t ca Ser act Th r gt a Vali cgc Arg tcc Se r aaa L ys tcc Ser gcc Aia caa Gin gga Gly cta Leu gtt Val aat As n caa Gin act Thr gga Giy cg c Arg tct Ser gcc Ala gtg Val gaa Giu 1 cg C Arg atc Ile aaa Lys ggc Gly cg c Arg aac As n ctg Le u aaa Lys aac Asn tcc Ser tct Ser ctq Leu cgc Arg ctt Leu tca Ser tgc Cys acc Th r gt a Vali ttg Leu act Th r g ca Ala tac Tyr qqC Gly atc Ile gaa Giu ccg Pro gat Asp 100 gaa Glu WO 01/00805 PCT11B00100926 105 110 115 aaa ctt ccc gac tcc gac gcc gcc tgg ccc ctc acg ctc tcc ggc ggc 499 Lys Leu Pro Asp Ser Asp Ala Ala Trp Pro Leu Thr Leu Ser Gly Gly 120 125 130 caa gcc cag cgc gtc tcc ctt gcg cga gcg ctc atc tcc gag cca gag 547 Gin Ala Gin Arg Vai Ser Leu Ala Arg Ala Leu Ilie Ser Giu Pro Glu 135 140 145 ctt ttg ctt ctc gac gaa ccc ttc ggc gcc ctc gat gct ctg aca aga 595 Leu Leu Leu Leu Asp Glu Pro Phe Gly Ala Leu Asp Ala Leu Thr Arg 150 155 160 165 ctg aca gcc caa gac ctg ctg ctc aaa acc gtg aac acc cga aac ttg 643 Leu Thr Ala Gin Asp Leu Leu Leu Lys Thr Val Asn Thr Arg Asn Leu 170 175 180 gga gtt ctg ctg gtc acc cat gat gtt tcc gag gcc atc gcc ctg gcc 691 Gly Val Leu Leu Val Thr His Asp Val Ser Giu Ala Ile Ala Leu Ala 185 190 195 gac cac gtc ctt ctt ctt gac gac qqc gcc atc aca cac agt ttg act 739 Asp His Val Leu Leu Leu Asp Asp Gly Ala Ile Thr His Ser Leu Thr 200 205 210 gta gat atc ccc ggc gat cgc cgc acc cac ccc tcc ttt gcc tcc tac 787 Val Asp Ile Pro Gly Asp Arg Arg Thr His Pro Ser Phe Ala Ser Tyr 215 220 225 acc gct caa ctc ctt gag tgg ctc gaa atc acc aca cct gcc 829 Thr Ala Gin Leu Leu Giu Trp Leu Glu Ile Thr Thr Pro Ala 230 235 240 tagaaagaaa tcatgaaatt taa 852 <210> 368 <211> 243 <212> PRT <213> Corynebacterium glutamicum <400> 368 Met Thr Ala Thr Leu Ser Leu Lys Pro Ala Ala Thr Vai Arg Gly Leu 1 5 10 Arg Lys Ser Tyr Gly Thr Lys Glu Val Leu Gin Gly Ile Asp Leu Thr 25 Ile Asn Cys Giy Glu Val Thr Ala Leu Ile Gly Arg Ser Gly Ser Gly 40 Lys Ser Thr Ilie Leu Arg Val Leu Ala Gly Leu Ser Lys Glu His Ser 55 Gly Ser Val Giu Ile Ser Gly Asn Pro Ala Vai Ala Phe Gin Giu Pro 70 75 Arg Leu Leu Pro Trp Lys Thr Val Leu Asp Asn Val Thr Phe Gly Leu 90 WO 01/00805 Arg Thr Asp 100 Leu Ala Glu 115 Leu Ser Gly 130 Ser Glu Pro Ala Leu Thr Thr Arg Asn 180 Ile Ala Leu 195 His Ser Leo 210 Phe Ala Ser Pro Ala Al a Ser Val1 Asp Asp 170 Val1 Leo Gly Leu PCTJIBOO/00926 Al a Leo Leu Leu 160 Val1 Glu Ile Pro Th r 240 <210> 369 <211> 1080 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1057) <223> RXN02613 <400> 369 agatatcccc ggcgatcgcc gcacccaccc ctcctttgcc tgagtggctc gaaatcacca cacctgccta qaaagaaatc atc Ile gc t Ala aaa Lys ctc gtt Val ga t Asp ctt Leo tca ggc Gly ga t Asp atc Ile gtc tcctacaccg ctcaactcct atg aaa ttt aag aaa Met Lys Phe Lys Lys 1 gca tcc tgc tca tca Ala Ser Cys Ser Ser tcc atc gat ctg agc Ser Ile Asp Leo Ser gga aca gaa caa gtg Gly Thr Glu Gin Val tat aaa atc gaa tgg 115 163 211 259 307 WO 01/00805 Leu Gin Ala Ser Gly Giu Leu Asp Asp Val Pro PCT/EBOO/00926 tea Ser caa Gin ccc Pro ga t Asp ctt Leu cac His 150 gaa Giu Gly gaa Giu ggg Giy aag Lys 230 gaa Glu gaa Giu cg c Arq ctt Leu tt t Phe ga t Asp aac As n atc Ile 120 gga Gi y ate Ile aac Asn gta Val1 gaa Giu 200 gga Gly gee Aila gct Ala ggc Gly cag Gin 280 ga c Asp acc Tb r ttc Ph e acc Th r 105 ttg Leu aa g Lys caa Gin ctc Leu ga t Asp 185 gga Giy ttt Phe gc Aia ga a Glu ttt Phe 265 gt 9 Vali get Aila tct Ser gcg Aia a aa Lys gte Vali aaa Lys caa Gin etc Leu 170 geg Aia gct Aia ggt Giy ttg Le u gac Asp 250 gat Asp c cg Pro ttc Ph e gga Giy 75 ate Ile gtg Val1 gce Aia gtg Vali eta Leu 155 caa Gin t gg Trp caa Gin gte Vai gca Ala 235 aac As n cc-g Pro etc Leu gte Vali eCa Pro ace Th r gte Vali C eq Pro get Ala 140 gaa Giu ccc Pro gca Ala gt t Vali gca Ala 220 ga t Asp a cc Tb r gag Giu ga c Asp tee Se r Ca a Gin aac As n gee Aia i10 tet Ser gcc Al a gca Ala gac Asp t gg T rp 190 gt C Val ga t Asp ct C Leu ga a Giu tet Ser 270 tee Se r gg t Gi y ate Ile acc Th r 95 tac Tyr tca Ser ege Ar g ggc Gly gc c Ala 175 ga t Asp agg Arg ga a Giu ga t Asp tgg Trp 255 caa Gin gtc Vali cte Leu ga a Giu 80 cca Pro aac As n ata Ile gga Giy gtg Val1 160 aaa Lys ccc Pro gga Gly g eq Ala eqe Arg 240 geg Ala ctg Leu aae As n gtt Val1 Tyr g ca Ala e eq Pro aac Asn ace Thr tee Ser 145 age Ser gee Ala tac Tyr geg Ala etc Leu 225 gtg Vai a eq Thr aa e As n ace Thr gag Glu Lys Ile Giu Trp tta Leu ate Ile gat Asp tcg Se r 130 age Ser gtt Val1 get Ala age Ser g ga Giy 210 ga t Asp gee Al a att Ile ace Tb r tat Tyr 290 gac Asp aac Asn ate Ile get Ala 115 g tg Val1 gee Ala gac Asp tte Phe tea Ser 195 e tg Le u ga c Asp gac Asp ttc Phe ege Arg 275 cag Gin ttt Phe gea Al a gge Gly 100 tta Le u get Ala cac His ga C Asp caa Gin 180 eag Gin gte Val ccc Pro tet Se r age Ser 260 age Ser aae Asn aat Asn ggt Gly Giy ggt Gly gae Asp g ga Gly gta Val 165 aae Asn geg Ala agt Ser gea Ala tat Tyr 245 eaa Gin etg Leu g eq Ala tte Phe 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 WO 01/00805 295 300 305 gag qac acc gta gac acc cga ttt gag ggc taagtatgtc tgagtatggc Glu Asp Thr Val Asp Thr Arg Phe Giu Gly 310 315 aa a <210> 370 <211> 319 <212> PRT <213> Corynebacterium glutamicum PCTIIBOO/00926 1077 1080 <400> 370 Met 1 Al a Ser Gly Tyr Ala Pro Asn Thr Ser 145 Se r Ala T yr Ala Leu Lys Ser Ile Thr Lys Leu Ile Asp Ser 130 Ser Val Ala Se r Gly 210 Asp Phe Cys Asp Giu Ile Asn Ile Ala 115 Val Ala Asp Phe Ser 195 Le u Asp Lys Ser Leu Gin G 1 uv Ala Gly 100 Le u Ala His Asp Gin 180 Gin Val1 Pro Lys Ile Ala Leu Val 5 Ser Ser Val T rp Gly Gly Gly Asp Giy Val 165 Asn Ala Ser Ala Al a Ly s Le u Se r 70 Gin Pro Asp Leu His 150 Glu Gly Giu Gly Ly s 230 Se r Val1 Gin 55 Ser Ile Th r Val Ala 135 Le u Ilie Gin Leu His 215 Giu Gly Th r 40 Ala Phe Asp Asn Ile 120 Gly Ile Asn ValI Glu 200 Gly Ala Asp 25 Leu Ser Thr Phe Th r 105 Leu Ly s Gin Leu Asp 185 Gi y Phe Ala Le u 10 Pro Asn Gly Ser Ala 90 Lys Val1 Lys Gin Leu 170 Ala Ala Gly Leu Asp Ph e Thr Gi y Le u Pro Th r Val Pro Ala 140 Giu Pro Ala Val1 Ala 220 Asp Th r Gly Asn Asp Asp Pro Gly Ser Asp 125 ValI Lys Ser Val1 Leu 205 Ser Phe Asp Leu Ala Gin Asp Gin As n Al a 110 Ser Ala Ala Asp Trp 190 Val Asp Le u Glu Gly Asp Ilie Val Ile Thr Tyr Ser Arg Gly Ala 175 Asp Arg Glu Asp T rp Leu Gly Ala Pro Glu Pro As n Ile Giy ValI 160 Lys Pro Gly Al a Arg 240 Al a 225 Val Ala Asp Ser Tyr Glu Trp Ala Giu WO 01/00805 PCTfIBOO/00926 245 250 255 Thr Ilie Phe Ser Gin Glu Ser Gly Phe Asp Pro Giu Ala Ser Gin Leu 260 265 270 Asn Thr Arg Ser Leu Arg His Gin Val Pro Leu Asp Giu Ser Val Asn 275 280 285 Thr Tyr Gin Asn Ala Leu Ilie Asp Ala Phe Val Ser Ala Gly Leu Val 290 295 300 Giu Asp Phe Asn Phe Giu Asp Thr Val Asp Thr Arg Phe Giu Gly 305 310 315 <210> 371 <211> 1698 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1675) <223> RXN00368 <400> 371 ttcttccaaa acgcaatgaa ctagttttcc tatqcagtta tctccttcaa atacgtcgac gcagcgcttt cacatctctg cgttgacgtc tttgtctttc atg cgt ctg Met Arg Leu ggt gtq Gly Val t gg Trp ggc Gly ttg Le u gt g Vai act Th r ctg Leu ggg Giy att ctg Leu ttg Leu ggC Gly 9gC Giy ga t Asp ttq Leu aag Lys tac ttg Leu aat Asn aat Asn 99C Gly acc Thr att Ile aac Asn gtg ttt Phe cag Gin tcc Ser a cg Thr gcg Aila ggg Gly Cgg Arg aca atc Ile ttt Phe gcc Aila atc Ilie ttg Le u qct Ala ttt Phe 110 ttt ct g Le u ga t Asp ct t Leu ctg Leu tta Le u ca g Gin gt g Va i 115 cac gtg Val1 t cc Se r tet Ser gac Asp tcc Ser ctg Leu 100 t gg Trp tcc gtg Val gga Gly gcg Ala cga Arg ccg Pro ttc Phe ga t Asp tat wo 01/00805 PCT[IBOO/00926 Ilie Tyr Gly Ala Asp Giy Val Thr Phe Leu Leu Ile Val His Ser Tyr ccc Pro gat Asp 150 ttg Leu ttt Ph e ctg Le u cgc Arg acc Thr 230 ga t Asp a ca Thr atc Ile ctg Leu gac Asp 310 gta ValI cta Leu a cc Th r act Th r 135 t tg Leu cgc Arg act Thr ttg Leu ttc Phe 215 att Ile tat Tyr ccg Pro acg Th r gca Ala 295 aac Asn atc Ile atc Ilie cag Gin 120 gtg Val1 gag Giu acc Th r ctt Leu gga Gly 200 a tg Met gg c Gly ctq Leu cat His t gg T rp 280 tac Tyr atc I le gaa Giu tgt Cys cat His atc Ile gct Ala aca Thr 170 aca Th r cca Pro tcc Ser gtg Val1 tct Ser 250 ttt Phe atc Ile g ca Ala ctc Le u ttc Ph e 330 gtg Val1 gcc Ala att Ile gca Ala 155 ctc Leu gtg Val gcg Ala ggc Gly ttg Le u 235 t tg Leu act Thr gcg Al a tta Le u aac As n 315 agc Ser ctg Le u aac Asn gtt Val 140 c99 Arg cca Pro 9c9 Ala cg t Arg a cc Th r 220 ttg Leu tac Tyr ctc Le u ttg Leu ctg Le u 300 aa c Asn aac As n gg a Gly gta Val1 125 tcg Ser atc Ilie ctg Leu aac As n ttt Ph e 205 gtg ValI ttc Phe g cg Al a aac Asn atc Ilie 285 cct Pro ttt Phe tcc Ser t gg Trp c cg Pro gca Ala 9cg Ala ctc Leu ctc Le u 190 gaa Glu agc Ser ctg Le u gca Ala aaa Lys 270 atc Ile g cc Al a gaa Giu ctc Le u ctg Leu 350 ttg Le u gct Ala ggg Gly aaa Lys 175 gcc Ala acc Th r aat Asn g ga Gly tca Ser 255 tca Ser acc Thr cca Pro g ca Ala atg Met 335 atc Ile aca Th r ctg Le u g cg Ala 160 cct Pro gac Asp tta Leu cca Pro atc Ile 240 aag Lys cg a Arg gcc Ala gg t Gly gca Ala 320 tta Leu gg a Gly 130 caa Gin act Thr t tg Leu ggc Gly acc Thr 210 cag Gin q ca Ala caa Gin cca Pro c cg Pro 290 ccg Pro agc S er ctg Leu ctc Leu ct t Leu ttt Phe ttg Leu att Ile 195 atg Met gtg Val1 gt a Val1 gac Asp gtc Val 275 ct g Leu ttc Ph e aat As n ggt Gly atc Ile 355 cct Pro a cg Thr tcg Se r 180 cca Pro att Ile gt a Val acc Th r gca Ala 260 ag c Ser ctg Leu aac Asn cca Pro gca Al a 340 acc Thr agt S er gtg Val1 165 g cg Ala g ct Ala tat Tyr tcc Ser gcg Ala 245 gga Gly gtg Val ggt Gly c ta Le u cg a Arg 325 gcc Ala cqa Arg 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 ctc act gtg ctg ctt ccc Leu Thr Val Leu Leu Pro WO 01/00805 PCTIIBOO/00926 360 365 370 acc gca ctg ccg ggc atg atc atc ggc gtc ggc tgg ctc att ttg ggc 1267 Thr Ala Leu Pro Gly Met Ile Ile Gly Val Gly Trp Leu Ile Leu Gly 375 380 385 aga tac acc gga att tac aac aca cct tgg gtg att ttg ggt gca tat 1315 Arg Tyr Thr Gly Ile Tyr Asn Thr Pro Trp Val Ile Leu Gly Ala Tyr 390 395 400 405 gtg tgt gct ttt acc gcg ctg gtt gtc caa gct gta cgc gga cca ctc 1363 Val Cys Ala Phe Thr Ala Leu Val Val Gln Ala Val Arg Gly Pro Leu 410 415 420 agt caa gca ccc gaa gca atc gaa gaa gcc gca cga atc agc ggc gca 1411 Ser Gin Ala Pro Glu Ala Ile Glu Glu Ala Ala Arq Ile Ser Gly Ala 425 430 435 ggc aga tta cga tcc atc atg gac acc acc gga gcg atg gca att ccc 1459 Gly Arg Leu Arg Ser Ile Met Asp Thr Thr Gly Ala Met Ala Ile Pro 440 445 450 gca gct ttc gcc ggc gca gtg ctg gtt gcg gta act gcg gtt cga gag 1507 Ala Ala Phe Ala Gly Ala Val Leu Val Ala Val Thr Ala Val Arg Glu 455 460 465 tta acc qtg tcc att ttq ctc atc gcg ccg ggc acc acc acc ctg ggt 1555 Leu Thr Val Ser Ile Leu Leu Ile Ala Pro Gly Thr Thr Thr Leu Gly 470 475 480 485 gtg cag qtg ttc aat ttg cag cag gcg gga aat tac aat cag gca tcg 1603 Val Gin Val Phe Asn Leu Gin Gin Ala Gly Asn Tyr Asn Gin Ala Ser 490 495 500 gcg ttg tcg ttg atg ttt gcg att atc ggt atc gtg gcq ctc gcg ttg 1651 Ala Leu Ser Leu Met Phe Ala Ile Ile Gly Ile Val Ala Leu Ala Leu 505 510 515 acg gtg cgc aqc cag aag gag ttt taggtgtcat cgatcaaatt gcg 1698 Thr Val Arg Ser Gin Lys Glu Phe 520 525 <210> 372 <211> 525 <212> PRT <213> Corynebacterium glutarnicum <400> 372 Met Arg Leu Gly Val Trp Leu Ile Val Ala Gly Leu Phe Ile Thr Pro 1 5 10 Leu Ala Leu Val Val Gly Leu Ala Leu Gly Gly Asn Gin Phe Pro Ala 25 Leu Trp Asp Ser Gly Leu Gly Lys Ala Leu Trp Asn Ser Ala Tyr Thr 40 Thr Val Leu Ser Ala Val Gly Ala Thr Ile Ile Gly Thr Ile Met Ala 55 WO 01/00805 WO 0100805PCT/IBOO/00926 Leu Thr Leu Asp Arg Thr Asp Val Phe Gly Phe Trp Thr Ile Ar g 145 Asp Ala Phe Al a Le u 225 Ala Le u Ile Ala Val 305 Leu Se r Val1 Thr T rp Leu Leu Glu Val1 130 Gin Th r Leu Giy Thr 210 G ln Ala Gin Pro Pro 290 Pro Ser Leu Leu Vali 370 Leu Leu Gin Val1 115 His Leu Phe Leu Ile 195 Met Vali Val1 Asp ValI 275 Leu Ph e As n Gly I le 355 Le u Ile Ser Leu 100 Trp Se r Pro Thr Ser 180 Pro Ile Vali Thr Ala 260 Se r Leu As n Pro Al a 340 Thr Leu Leu Pro Ph e Asp T yr Ser Vai 165 Ala Ala Tyr S er Ala 245 Gly Val1 Giy Leu Arg 325 Ala Arg Pro Giy 70 Le u Gly Ile Pro Asp 150 Leu Phe Le u Arg Th r 230 Asp Th r Ile Le u Asp 310 Val Leu Th r Th r Arg Leu Lys T yr.

Th r 135 Leu Arg Thr Leu Phe 215 Ile Tyr Pro Thr Ala 295 Asn Ile Ile Gin Al a 375 Tyr I le Asn Gly 120 Val1 Giu Thr Leu Gi y 200 Met Gly Le u His Trp 280 Tyr Ile Giu Cys His 360 Leu Th r Pro Gin 105 Ala Tyr Gin Ile Th r 185 Ser Glu Ile Val Arg 265 Ile Arg Thr Gly Gly 345 Phe Pro Gly Pro 90 Gi y Asp Ile Ala Th r 170 Th r Pro Ser Val1 Ser 250 Ph e Ile Ala Leu Phe 330 ValI Ala Gi y Ile Arg 75 Phe Ile Gi y Ile Ala 155 Leu Val1 Al a Gly Leu 235 Leu Thr Al a Le u As n 315 Ser Leu As n Met Tyr Ile Asn Val1 ValI 140 Arg Pro Ala Arg Thr 220 Leu Tyr Le u Le u Leu 300 Asn Asn Gly ValI Ile 380 Asn Thr Ala Leu Arg Leu Giy Arg Thr 125 Ser Ile Leu Asn Phe 205 Val1 Phe Ala As n Ile 285 Pro Phe Se r Trp Pro 365 Ile Th r Ala Phe 110 Ph e Ala Al a Leu Le u 190 Giu Ser Leu Al a Lys 270 Ile Ala Giu Le u Leu 350 Leu Gly Pro Ile Phe Leu Ala Gly L ys 175 Ala Thr Asn Gi y Ser 255 S er Thr Pro Ala Met 335 Ile Thr Val1 T rp Ala Gly Leu Leu Ala 160 Pro Asp Le u Pro Ile 240 Lys Arg Ala Gly Ala 320 Le u Gi y Leu Gly Val WO 01/00805 PCT/IBO0/00926 385 390 395 400 Ile Leu Giy Ala Tyr Vai Cys Ala Phe Thr Ala Leu Val Val Gin Ala 405 410 415 Val Arg Gly Pro Leu Ser Gin Ala Pro Glu Ala Ile Giu Giu Ala Ala 420 425 430 Arg Ilie Ser Gly Ala Gly Arg Leu Arq Ser Ile Met Asp Thr Thr Gly 435 440 445 Ala Met Ala Ile Pro Ala Ala Phe Ala Gly Ala Val Leu Val Ala Val 450 455 460 Thr Ala Val Arq Glu Leu Thr Vai Ser Ile Leu Leu Ile Ala Pro Gly 465 470 475 480 Thr Thr Thr Leu Gly Val Gin Val Phe Asn Leu Gin Gin Aia Gly Asn 485 490 495 Tyr Asn Gin Ala Ser Ala Leu Ser Leu Met Phe Ala Ile Ile Gly Ile 500 505 510 Val Ala Leu Ala Leu Thr Val Arg Ser Gin Lys Giu Phe 515 520 525 <210> 373 <211> 602 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (579) <223> FRXA00368 <400> 373 tcc ctc atg tta tcc ctg ggt gca gcc cta atc tgt gqg gtg ctg gga 48 Ser Leu Met Leu Ser Leu Giy Ala Ala Leu Ile Cys Gly Val Leu Gly 1 5 10 1 tgg ctg atc gga gtg ctc atc acc cqa acc cag cat ttc gcc aac gta 96 Trp Leu Ile Gly Val Leu Ile Thr Arg Thr Gin His Phe Ala Asn Val 25 ccg ttg aca ctc act gtg ctg ctt ccc acc gca ctg ccg ggc atg atc 144 Pro Leu Thr Leu Thr Val Leu Leu Pro Thr Ala Leu Pro Gly Met Ile 40 atc ggc gtc ggc tgg ctc att ttg ggc aga tac acc gga att tac aac 192 Ile Giy Val Gly Trp Leu Ile Leu Gly Arg Tyr Thr Gly Ile Tyr Asn 55 aca cct tgg gtg att ttg ggt gca tat gtg tgt gct ttt acc gcg ctg 240 Thr Pro Trp Val Ile Leu Gly Ala Tyr Val Cys Ala Phe Thr Ala Leu 70 75 gtt gtc caa gct gta cgc gga cca ctc agt caa gca ccc gaa gca atc 288 Val Val Gin Ala Val Arg Gly Pro Leu Ser Gin Ala Pro Giu Ala Ile 90 WO 01/00805 gaa gaa 9CC gca cga atc aqc ggc gca Giu Giu Ala Ala Arg Ile Ser Gly Ala 100 105 gac acc acc gga gcg atg gca att ccc Asp Thr Thr Gly Ala Met Ala Ilie Pro 115 120 ctg gtt gcg gta act gcg gtt cga gag Leu Val Ala Vai Thr Ala Val Arg Giu 130 135 atc gcg ccg ggc acc acc acc ctg ggt Ile Ala Pro Gly Thr Thr Thr Leu Gly 145 150 cag gcg gga aat tac aat cag gca tcg Gin Ala Gly Asn Tyr Asn Gin Ala Ser 165 att atc ggt atc gtg gcg ctc gcg ttg Ile Ilie Gly Ilie Val Ala Leu Ala Leu 180 185 ttt taggtgtcat cgatcaaatt gcg Ph e <210> 374 <211> 193 <212> PRT <213> Corynebacterium giutarnicum <400> 374 Ser Leu Met Leu Ser Leu Gly Ala Ala 1 5 Trp Leu Ile Gly Val Leu Ile Thr Arg 25 Pro Leu Thr Leu Thr Val Leu Leu Pro 40 Ilie Gly Vai Gly Trp Leu Ile Leu Gly 55 Thr Pro Trp Vai Ile Leu Gly Ala Tyr 70 Val Val Gin Ala Val Arg Giy Pro Leu Glu Giu Ala Ala Arg Ile Ser Giy Ala 100 105 Asp Thr Thr Giy Ala Met Ala Ile Pro 115 120 Leu Val Ala Val Thr Ala Val Arg Giu aga Arg gct Ala acc Thr cag Gin 155 ttg Leu gtg Val1 tta Le u ttc Ph e g t g Val1 140 g tg Val1 t cg Se r cgc Arg cga Arg 9CC Ala 125 tcc Ser ttc Phe ttg Leu agc Ser PCT/IBOO/00926 atg 336 Met gtg 384 Val ctc 432 Leu cag 480 Gin 160 gcg 528 Ala gag 576 Glu Leu Th r Thr Arq Val Ser Gly Ala Leu Ile Gin Ala Tyr Cys 75 Gln Arg Aia Thr Cys His Leu Thr Ala Ala Le u Phe ValI Gly Val Phe Ala Pro Gly Gly Ile Phe Thr Pro Giu Arg Ser 110 Ala Gly 125- Ser Ile Gly Val1 Ile Asn Leu Ile Met Val Leu WO 01/00805 PCTfIBOO/00926 130 135 140 Ile Ala Pro Gly Thr Thr Thr Leu Gly Val Gin Val Phe Asn Leu Gin 145 150 155 160 Gin Ala Gly Asn Tyr Asn Gin Ala Ser Ala Leu Ser Leu Met Phe Ala 165 170 175 Ile Ile Gly Ile Val Ala Leu Ala Leu Thr Val Arg Ser Gin Lys Glu 180 185 190 Phe <210> 375 <211> 798 <212> DNA <213> Corynebacteriut giutamicum <220> <221> COS <222> <223> FRXA00370 <400> 375 9gC Gly 1 99 C Gly ga t Asp ttg Le u aag Lys tac Tyr act Tb r ttg Leu C9 C Arg aaa 9CC Lys Ala gcq acc Ala Thr gtt ttc Val Phe atc cct Ile Pro aac cag Asn Gin 99C gct Gly Ala gtg tac Val Tyr gag caa Giu Gin 115 acc atc Thr. Ile 130 aat tcc 9CC tat aca aca gtg ctt tct gcg gtg Asn Ser Ala Tyr Thr Thr Val Leu Ser Ala Val 10 99 C Gly acc Tb r att Ile aac Asn gtg Val1 gtt Val1 C9 Arg cca Pro ga c Asp tcc Se r ct 9 Le u tgg Trp tCC Ser c ct Pro 110 a cg Th r t C9 Ser WO 01/00805 WO 0100805PCTJLBOOIO0926 act Thr 145 t tg Leu t tc Phe att Ile tat Tyr ccg Pro 225 a cg Thr gca Al a 9c9 Ala 150 cgt Arg acc Thr ttq Leu tac T yr ct C Leu 230 ttg Le u ctg Le u ga c Asp t ta Leu 170 c ca Pro atc Ile aag Lys cga Arg gCC Al a 250 ggt Gi y Th r 10 Ala Le u Ala Gly Le u 90 Le u cca Pro att Ile gta Val1 190 acc Thr g ca Ala agc Ser Ctg Leu gCt Ala tat Tyr 175 tCC S er gc9 Ala gga Gly gt g ValI ggt Gly 255 ctg Leu 160 cgC Arg acc Th r gat Asp aca Th r atc Ile 240 ct g Le u 480 528 576 624 672 720 768 <210> 376 <211> 266 <212> PRT <213> Corynebacterium glutamicun <400> 376 Gly Lys Ala Leu Trp Asn Ser Ala Tyr 1 5 Gly Ala Thr Ile Ilie Gly Thr Ilie Met 25 Asp Val Phe Gly Arg Thr Ala Leu Arg Leu Ile Pro Pro Phe Ile Gly Ala Ile Lys Asn Gin Gly Ilie Asn Arg Phe Phe Tyr Gly Ala Asp Gly Val Thr Phe Leu Thr Val Tyr Ilie Ilie Val Ser Ala Ala 100 105 Ser Asp Ser Leu T rp Se r Pro 110 Al a Arg Pro Phe Asp T yr Se r WO 01/00805 Leu GluI Arg Thr 130 Thr Leu 145 Leu Gly Phe Met Ile Gly Tyr Leu 210 Pro His 225 Thr Trp Ala TyrI Arg Pro Ala 150 Arg Th r Le u Tyr Leu 230 Le u Le u Ile Leu 135 Asn Phe Val1 Phe Ala 215 Asn Ile Pro Ala 120 Leu Le u Glu Se r Leu 200 Ala Ly s Ile Al a Gly Lys Ala Thr Asn 185 Gly Se r Se r Thr Pro 265 Ala Pro Asp Le u 170 Pro Ile Lys Arg Ala 250 Gi y Asp Ala Phe 155 Al a Leu Al a Leu Ile 235 Al a PCTJIBOO/00926 Val Leu Ala Phe Ala Leu 160 Tyr Arg 175 Ser Thr Ala Asp Gly Thr Val Ile 240 Gly Leu 255 <210> 377 <211> 749 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> <223> RXN01285 <400> 377 ctc aac gtc acc atc ccc gac aac Leu Asn Val Thr Ile Pro Asp Asn 1 aac ggc tgc ggc aaa tcc acc ctg Asn Gly Cys Gly Lys Ser Thr Leu aat ccg cag cac ggc aaa gtg ctt Asn- Pro Gin His Gly Lys Val Leu ttc aag cct aaa gag atc gcc cga Phe Lys Pro Lys Glu Ilie Ala Arg 55 tcc atc gcc cca gaa ggc atc cgg Ser Ile Ala Pro Giu Gly Ilie Arg 70 acc Th r gg t Gly ggt Gly ggc Gly ga t Asp gcc Ala ttc Phe Cgg Arg ctg Le u ctc Le u WO 01/00805 WO 0100805PCTJIBOO/00926 cgc Arg gac Asp gct Al a gtg Val1 ccc Pro 145 ctg Leu ga t Asp gat Asp gag Glu ccc gct Ala g cc Al a cgc Arg gcc Ala 130 acc Th r cg c Arg ctc Le u ggg Gly atg Met 210 gt c ccc tac caa agc Pro Tyr Gin Ser gtc gcg caa gcg Val Ala Gin Ala 100 ctc gtc gat gaa Leu Val Asp Giu 115 atg ttg ctc gcc Met Leu Leu Ala acc ttc ctc gac Thr Phe Leu Asp 150 gca ttc aac gag Ala Phe Asn Giu 165 aac caa gcc gcc Asn Gin Ala Ala 180 cac gta cat gcc His Val His Ala 195 gtt caa gga gtt Val Gin Gly Val aca gga acc ccc ctc ata caa caa tgg Leu Ile Gin Gin Trp cgc acc tcc Arg Thr Ser ctc Le u ct C Le u cag Gin 135 atc Ile gcc Ala cgc Arg a cg Thr ttt Phe 215 acc acc Thr caa Gin 125 ct t Leu ctc Leu act Thr atc Ile g tc Val 205 atc Ile Arg ga a Giu 110 cga Arg ctc Leu ttg Le u gt g Val1 gtg Va 1 190 tta Le u t cc Ser tct Ser gac Asp ctt Leu gtq Val1 ga c Asp gaa Giu ctt Leu 175 atg Met act Thr cca Pro cgc Arg ga a Giu gca Ala t gg T rp gag Giu ttg Leu 160 cac His aaa Ly s gc C Ala gac Asp gca Aia 240 288 336 384 432 480 528 576 624 672 720 749 Pro Val Thr Giy Thr Pro Thr Val Vai 225 -230 gga gct Gly Ala taagtagcta cccctccaac gga <210> 378 <211> 242 <212> PRT <213> Corynebacterium giutamicum <400> 378 Leu Asn Val Thr Ilie Pro Asp Asn Thr Phe Thr Ala Ile Ile Gly Pro 1 5 10 Asn Gly Cys Giy Lys Ser Thr Leu Leu Arg Giy Phe Ser Arg Val Leu 25 Asn Pro Gin His Gly Lys Val Leu Leu Asp Gly Arg Gin Leu Asp Ser 40 WO 01/00805 Phe Lys 1 Ser Ilie Arg Ala I Asp AlaI Ala Arg I Val Alat 130 Pro Thr 145 Leu Arg )1 Asp Leu Asp Gly I Glu Met 210 Pro Val 225 Gly Ala Ile Gly 70 Se r Al a Gi u Al a Asp 150 Glu Al a Al a Val Pro 230 Ala Ile Le u Le u Le u Gin 135 Ile Ala Ar g Th r Phe 215 Th r Arg Arq Ile Al a Ser 120 Gin Ala Gly T yr Gly 200 Gly Val Pro Ala Se r Glu 110 Arg Leu Leu Val Val 190 Leu Ser Ser PCTJIBOO/00926 Gin Thr Arg Gly Asp Glu Leu Ala Val Trp Asp Glu Glu Leu 160 Leu His 175 Met Lys Thr Ala Pro Asp Arg Ala 240 <210> 379 <211> 1149 <212> DNA <213> Corynebacteriun glutamicum <22 0> <221> CDS <222> (101)..(1126) <223> RXN00523 <400> 379 tggtgactcg tccgagtgaa attgccgtgg gcatcatcat gccgatcatt ggtgcgccac tgtttatttg gattattcgt cgtcagaaag tcaaagagct atg agc ctt agc cat 115 Met Ser Leu Ser His 1 caa ctc aag cgc cag cgc gca tcg cgc aac tcc cgc agg tgg ctg att 163 Gin Leu Lys Arg Gin Arg Ala Ser Arg Asn Ser Arg Arg Trp Leu Ile 15 wo 01/00805 WO 0100805PCT[IBOO/00926 gt t Val1 a tg Met tct Se r t tg Le u gc g Ala ga t Asp att Ile tgt Cys ggt Gly 150 9 C9 Ala t gg Trp gcg Ala att Ile gg c Gly 230 gt c Val1 gca 9 cg Ala tgg Trp gga Gly ccg Pro gyc Gly att Ile gtg Val1 g cg Al a 135 99 t Gly atg Met ga t Asp acg Thr ccg Pro 215 aat Asn att Ile t gc gca ttg ggc Ala Leu Gly ggc gag gtg Gly Glu Val cag cag gtt Gin Gin Val cgc gcg gtg Arg Ala Vai gtg att ttt Val Ile Phe atc gqc att Ile Giy Ile 105 ttt ttc ggg Phe Phe Gly 120 tcc ttg qct Ser Leu Ala ttt tcg gcc Phe Ser Ala ctg aat tca Leu Asn Ser 170 ctg ccg acc Leu Pro Thr 185 tgg gat cgt Trp Asp Arg 200 ctg ctg gtg Leu Leu Vai gat tcc gcg Asp Ser Ala gcg att att Ala Ile Ile 250 ggc ccg atc gtc gtc acg ctt Val Vai Thr Leu 30 ttt Ph e ccc Pro atg Met 75 cag Gin t ct Se r atg Met gtg Val1 a cg Thr 155 tta Leu gcg Ala gcg Ala gct Al a gtg Val1 235 gcc Ala gca tat Tyr ggc Gly 60 gg t Gly acg Th r t ct Ser t cg Se r g cg Al a 140 cg t Arg gtg ValI a cg Thr atg Met aat Asn 220 ggt Gly gct Ala ttc 9gc Gly 45 gcg Ala t tg Leu gtg ValI ggc G ly cag Gin 125 ttg Leu ct g Le u tcg Se r cgc Arg ccg Pro 205 gcg Al a ttg Leu gtt ValI gg cct Pro agt Ser act Thr ttg Leu gcg Al a 110 t ct Se r ttg Leu att Ile tat Tyr t gg T rp 190 ctg Leu cgc Arg 9gc Gly g cg Ala gcg ggt Gi y gct Ala tat Tyr gcg Ala cg t Arg 95 tcg Ser gca Ala att Ile ct t Leu t cg Se r 175 Ct t Le u at t Ile aa t As n gtt Val1 ctc Leu Ile Phe Ala P att ttt gct ttt tct ttg ca g Gin tcc Ser ggt Gly 80 aat As n g cg Ala gtg Val1 tat Tyr acc Th r 160 ctg Leu acc Th r gtc Val1 gtg Val1 gct Al a 240 atc Ile gtg Val1 gt t Val t tg Leu cag Gin gcg Al a tcg Se r ctg Leu 145 g gc Gi y t cc Ser ggc Gly a cc Thr ga t Asp 225 act Thr gcc Al a ctg Le u 9gc Gly g cg Ala ttg Leu gg c Gly gcg Ala 130 gtg Val1 att Ile aag Lys t cg Ser act Th r 210 ct t Leu aat As n gt t ValI a

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he Ser aa gtg ys Val tg ttg al Leu tt ggc he Gly cg tcg la Ser 100 ta att al Ile tt tct le Ser cg tat la Tyr gt att ly Ile ct gat la Asp 180 tc aat eu Asn tg gta al Val tg cgt et Arq gc acg rg Thr ct acc la Thr Leu t tg Leu cgt Arg g cg Ala ccg Pro tgc Cys ttg Leu cgc Arg gct Ala 165 tct Se r gg t Gly ct c Le u ttg Le u cg c Arg 245 gct Al a 211 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 255 260 ttt gtg tct qgc ccc att gcc WO 01/00805 Ala Cys Gly Pro Ile Ala Phe Val Ala 265 270 gcg cgc att tta 99c tcc ggc gga tcg Ala Arg Ile Leu Gly Ser Gly Gly Ser 280 285 atc gqc ggg ttg atc gtg ctc atc gcc Ile Gly Gly Leu Ile Vai.Leu Ile Ala 295 300 ctc ggc acc cgc tac ccc gtc gqa gtt Leu Gly Thr Arg Tyr Pro Vai Giy Val 310 315 cca ttc ctt atc tat tta ctc att cgt Pro Phe Leu Ile Tyr Leu Leu Ile Arg 330 ctg tgaccaccaa ccatcaacta tcc Le u <210> 380 <211> 342 <212> PRT <213> Corynebacteriun giutamicum <400> 380 Met Ser Leu Ser His Gin Leu Lys Arg Val1 atc Ile cta Le u acc Th r 320 aac As n Se r atc Ile att Ile 305 ggc Gly cgc Arg PCTJIBOO/00926 Ile Ala gca ctc 979 Ala Leu tac ttc 102' Tyr Phe ggc gcc 107 Gly Ala 325 gta acc 112 Val Thr 340 7 3 1149 1 Arg Phe Val1 Val1 Leu Gin Al a Se r Le u 145 Gly Arg Ala Leu Gly Al a Leu Gly Ala 130 Val Ile 5 Ile Le u Le u Arg Al a Pro Cys Le u Arg Ala ValI Met Se r Le u 70 Ala Asp Ile C ys9 Gly 150 Ala Ala Trp Gly Pro Gi y Ile Val1 Ala 135 Gly Met Al a Gi y Gin Arg Val1 Ile Ph e 120 Se r Phe Leu Gin 10 Gi y ValI Val1 Val Phe 90 Ile Gi y Al a Ala Se r Arg Val1 Ph e Pro Met 75 Gin Se r Met Val1 Th r 155 Le u Se r Thr Gly Al a Le u Val Gly Gin 125 Leu Leu Se r Arg Leu Pro Ser Thr Leu Al a 110 Ser Leu Ile T yr Asn Gi y Al a Tyr Ala Arg Se r Ala Ile Le u Ser Se r Ile Gin Ser Gly Asn Ala Val Tyr Th r 160 Le u WO 01/00805 Ser Lys Ala Asp Ser Trp Asp Leu Pro Thr Ala Thr Arg Gly Thr Asp 225 Thr Ala Se r Ile Ile 305 Gly Arg Ser Thr 210 Le u Asn Val1 Gly Pro 290 Gly Al a Ala Leu 195 Val1 Met Arg Ala Pro 275 Ser Gin Phe Gi y 180 Asn Val1 Arg Th r Thr 260 Ile Ala Tyr Gly Val1 340 Gly Le u Le u Arg 245 Ala Ala Leu Phe Al a 325 Th r Ala Ile Gly 230 Val Ala Ala Ile Le u 310 Pro Le u Thr Pro 215 Asn Ile Cys Arg Gi y 295 Gly Phe Trp 200 Le u Asp Al a Gi y Ile 280 Gly Th r Leu 185 Asp Leu Ser Ile Pro 265 Le u Le u Ar g Ile Arg Val Al a Ile 250 Ile Gly Ile Tyr T yr 330 Al a Ala Val 235 Al a Ala Se r ValI Pro 315 Leu Met Asn 220 Gly Al a Phe Gly Le u 300 Val Leu PCTIBOO/00926 175 Trp Leu Thr 190 Leu Ile Val Arg Asn Val Gly Vai Ala 240 Ala Leu Ile 255 Ala Phe Val 270 Ser Leu Ile Ala Asp Leu Val Val Thr 320 Arg Ser Asn 335 <210> 381 <211> 621 <212> DNA <213> Corynebacterium glutarnicum <220> <221> CDS <222> (101)..(598) <223> RXN01142 <400> 381 ctccccatcc accggcacag tcagcqcagg caacgaagaa attaaaggac caggacctqa ccgaggcatg gttttccaag accacgccct cctgccctqa ttg acc gca cgc ggc Leu Thr Ala Arg Gly 1 aac atc gac ttc ggg ctc cgc tcc gcg cgc ccc tcc ttg agc aaa acc Asn Ile Asp Phe Gly Leu Arg Ser Ala Arg Pro Ser Leu Ser Lys Thr 15 gaa cgc gcc gac atc acc cgc acc cac ctc gaa caa gta ggc ctc acc Glu Arg Ala Asp Ile Thr Arg Thr His Leu Giu Gln Val Gly Leu Thr 30 WO 01/00805 gac gcc g Asp Ala A~ cga gtc g Arg Val G ctc gac g Leu Asp G cag ctc c Gin Leu G atg gtc a Met Val T ctc gtg a Leu Val M1 1 gtg aat c Val Asn L 135 gaa 9CC g Glu Ala G icc gaa cgg la Glu Arg rgc atc gca ;y Ile Ala aa ccc ttc ;iu Pro Phe :aa cta ctc in Leu Leu cc cac gac 'hr His Asp 105 tg tcc aag let Ser Lys 20 tt ccc cgc *eu Pro Arg ag acc aca .lu Thr Thr CCC 9CC cgc Pro Aia Arg gcc ttc gcc Ala Phe Ala 9cc ctc gac Ala Leu Asp att tgg gaa Ile Trp Giu gac gag gcc Asp Glu Ala 110 ccc qaa 9CC Pro Glu Ala 125 aga cac gag Arg His Glu 140 ctg cgt aag Leu Arg Lys ct C Leu atc Ile 9CC Ala 9CC Ala 95 atc Ile acc Th r Ctg Leu C99 Arg t CC Se r gaC Asp ct C Le u 80 t CC Ser Ctg Le u atc Ile agt Ser atg Met PCTIBOO/00926 caa cag 259 Gin Gin atg Ctt 307 Met Leu gaa CtC 355 Giu Leu gtc gtC 403 Val Val 100 cga gtt 451 Arg Val att CCa 499 Ile Pro tCt gtt 547 Ser Val Ctg gag 595 Leu Glu 165 tagtttCtaa cacgtctttt aaa <210> 382 <211> 166 <212> PRT <213> Corynebacterium giutamiCum <400> 382 Leu Thr Ala Arg Gly Asn Ile Asp Phe 1 5 Ser Leu Ser Lys Thr Glu Arg Ala Asp Gin Val Gly Leu Thr Asp Ala Ala Giu 40 Gly Gly Met Gin Gin Arg Val Gly Ile Pro Pro Ile Met Leu Leu Asp Giu Pro 70 Thr Arg Arg Giu Leu Gin Leu Gin Leu Gly Ile Arg Ala Phe Leu Se r Thr Ala' Phe Le u Trp Pro Gi u Se r As p Le u Se r WO 01/00805 Arg Arg Thr Val 100 Leu Ser Asp Arg 115 Ile Thr Asp Ile 130 Glu Asp Ala Ser 145 Leu His Leu Leu PCTIBOO/00926 Val Met Val Thr His Asp Val Asp Glu Ala Ile Leu 105 110 Val Leu Val Met Ser Lys Ser Pro Glu Ala Thr Ile 120 125 Pro Val Asn Leu Pro Arg Pro Arg His Giu Leu Ser 13*5 140 Val Giu Ala Glu Thr Thr Ala Leu Arg Lys Arg Met 150 155 160 Glu His <210> 383 <211> 948 <212> DNA <213> Corynebacterium glutarnicum <220> <221>'CDS <222> (925) <223> RXN01141 <400> 383 aaagaacact cggtatggca cctgatttaa ggatgctgca atcgtgacac atatcctctt cgacagcagg cgttttctgc aactgggcgc ttttgcgtcc ttg agc acc gca ttg Leu Ser Thr Ala Leu gga Gly aac Asn att Ile gtc Val1 gca Al a gcg Ala ttt P he gcg Al a act Thr gct Ala a cg Thr aca Thr att Ile acc Th r gtg Val1 att Ile Al a tac T yr ga t Asp gtg Val1 aat As n acq Thr 9gC Gly ct a Le u tca Se r gt t ValI acc Th r 99g Gly t cg Ser tac Tyr 9gg Gly ggc Gly g cg Ala aac Asn caa Gin 110 aca Th r qt g Val1 ct g Le u tgg Trp ca C His gcg Ala gca Ala C ct Pro t cg Ser ggc Gly acc Thr atg Met gag Glu 100 tca Se r gcq Al a gcg Al a gtg ValI gcc Ala act Thr ct 9 Le u aag Lys 115 163 211 259 307 355 403 451 WO 01/00805 WO 0100805PCTIBOOIOO926 cac His gga Gly ctc Le u 150 ct c Leu gga Giy tcc Ser tgc Cys gcg Ala 230 agc Se r aaa 99 t Gly 120 ct Pro t ca Ser cct Pro att Ile cg g Arg 200 gCC Ala ca g Gin ccg Pro ctc tc Se r ttt Phe aac Asn gCC Aia 999 Gly 185 att Ile gtg Vali gqt Giy g ca Ala aac gt c ValI gaa Giu gca Al a ga t Asp 170 C ct Pro tgg Trp g cg Ala gtg ValI caa Gin 250 cag aat Asn tat Tyr gt t ValI 155 atg Met 999 Gly ctg Le u atg Met ctt Le u 235 ttt Ph e cct gcg Ala 125 gtc Val1

CCC

Pro gca Ala ttt Phe acc Th r 205 aaa Lys gcg Ala t CC Ser acg Thr ctt aaa ggc Leu Lys Gly 130 ctg ctc ctg Leu Leu Leu 145 gat ctt gag Asp Leu Giu 160 aca gtt gag Thr Val Glu cgc gcc atc Arg Ala Ile ctg t gg gac Leu Trp Asp 210 aaa gct gaa Lys Ala Glu 225 gaa gcc tcc Giu Ala Ser 240 cgc acg ctq Arg Thr Leu gat gga ccg Asp Gly Pro atg gtg ctg Met Val Leu cgc gat tat Arg Asp Tyr ctt cgc Ctg Leu Arg Leu 165 ggc atc gat Gly Ile Asp 180 agc aat ggc Ser Asn Gly 195 aaa cat cca Lys His Pro cac ccc acg His Pro Thr gca att ttg Ala Ile Leu 245 tcg cag gaa Ser Gln Glu 260 t cg Ser 275 Lys Tyr Leu Asn Gin Pro Ala 265 taatcatcgg catcaccggc tta <210> 384 <211> 275 <212> PRT <213> Corynebacterium glutamicum <400> 384 Leu Ser Thr Ala Leu Ala Gly Ala Ala Arg Tyr Vai Thr Ser Thr Ser 1 5 10 Asn Asn Glu Pro Ala Asp Asn Thr Pro Leu Thr Ile Gly Tyr Val Pro 25 Ile Ala Gly Ser Ala Pro Ile Ala Ile Ala Asp Ala Leu Gly Leu Phe 40 Lys Lys His Gly Val Asn Val Thr Leu Lys Lys Tyr Ser Gly Trp Ser 55 WO 01/00805 PCT/IBOO/00926 Ala Tyr Ala Thr Giu Gin Leu Asp Val Ala His Met Asp Leu Arg Thr Lys Le u 145 Le u Val1 Al a Trp Al a 225 Al a Th r Ci y Leu Ser Pro Leu Gl y 130 Leu Giu Giu Ilie Asp 210 Glu Ser Le u Pro Trp Thr Pro Met Thr Giu 100 Ala Ser 115 Met Val Arg Asp Leu Arg Gly Ile 180 Ser Asn 195 Lys His His Pro Ala Ile Ser Gin 260 Se r 275 70 75 Thr Leu Lys Leu Tyr Le u 165 Asp Gly Pro Thr Leu 245 Glu Ala Phe Tyr Ile 135 ValI Arg Phe Gly C ys 215 Ala Asn Tyr Ile Th r Ci y 120 Pro Se r Pro Ile Arg 200 Ala Gin Pro Leu As n Gin 105 Ser Phe As n Ala Gly 185 Ile ValI Gi y Ala As n 265 Ala 90 Asn Val1 Glu Al a Asp 170 Pro Trp Ala Val1 Gin 250 Gin Ala Ala Asp Al a Ala Leu 175 Civ Gin Trp Giu Ala 255 Le u Se r Ile Leu Leu Asp 160 Thr Arg Le u Lys Glu 240 Arg Asp <210> 385 <211> 927 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (904) <223> RXN01002 <400> 385 gactgctgat accgcacagg atgaaatcac tcgttacggc gagatcctga agaagttctc caactaattt ccctgtttcc aatactcaag gtgtgcgcat atg aat tct gat gct 115 Met Asn Ser Asp Ala 1 tcg gct acc acc aac tcc tgg gct atc aac ttc gac cat gtg tcg gtg 163 WO 01/00805 Ser Ala Thr acg tat ccc Thr Tyr Pro aat ccc ggt Asn Pro Gly tcc acg ctg Ser Thr Leu acc gtg acg Thr Val Thr ctg cgt gat Leu Arg Asp ctq tcg gaa Leu Ser Glu gcg cac aca Ala His Thr 120 gac aag cag Asp Lys Gin 135 aaa gtg tgg Lys Val Trp 150 gtt gct att Val Ala Ile gat qag cct Asp Glu Pro cgc gat cta Arg Asp Leu 200 aac ttg cac Asn Leu His 215 ggt ttg cgt Giy Leu Arg 230 acc gat aaa Thr Asp Lys Thr aat Asn gag Giu att Ilie gtg Val1 gCC Al a cgc Arg 105 gcg Al a att Ile acc Thr gcg Ala gt g Val1 185 ga a Glu ttg Le u gCC Ala ga c Asp As n ggq Gly atg Met cgc Arg ggg Gi y cg t Arg agc Se r t gg Trp gct Ala cga Arg cgc Arg 170 qca Ala aac Asn att Ile ggC Gly ttt Phe Asn 15 gat Asp ggt Gly ctt Leu aac As n atg Met 95 aat Asn ctc Leu gag Glu tcg Se r gat Asp 175 cca Pro gaa Giu caa Gin gac Asp ggt Gly Ph e ga t Asp ctg Le u gt C Val1 acc Th r 80 att Ile gtt Val ggg Gly tcc Ser ggt Gly 160 cc g Pro acc Thr 9gC Gi y tac Tyr gg t Giy 240 cgc Arg Asp gtt ValI t ca Ser cg c Arg ttg Leu ttc Phe ttg Le u ttt Phe gtg ValI 145 gga Gly tct Ser g cg Ala ct c Le u acc Th r 225 cct Pro ccc Pro PCTIBOOIOO926 Ser Val acc atc 211 Thr Ile ggt aaa 259 Gly Lys gaa ggc 307 Giu Gly aaa gca 355 Lys Ala ttc aac 403 Phe Asn 100 cgc ttc 451 Arg Phe gag cac 499 Giu His ttg cac 547 Leu His cag cgc 595 Gin Arg 165 ctg gca 643 Leu Ala 180 gtg atg 691 Val Met ttg gtg 739 Leu Val ctt gtg 787 Leu Val gag gcc 835 Giu Ala 245 gct aaa 883 Aia Lys WO 01/00805 PCT/IBOO/00926 250 255 260 gac ctg cta ggt gat cgc gca tgaccacgcc ttcttctaca ctt 927 Asp Leu Leu Gly Asp Arg Ala 265 <210> 386 <211> 268 <212> PRT <213> Corynebacterium glutamicum <400> 386 Met Asn Ser Asp Ala Ser Ala Thr Thr Asn Ser Trp Ala Ile Asn Phe 1 5 10 Asp His Val Ser Val Thr Tyr Pro Asn Gly Thr Lys Ala Leu Asp Asp 25 Val Ser Leu Thr Ile Asn Pro Giy Giu Met Val Ala Ile Val Gly Leu 40 Ser Gly Ser Gly Lys Ser Thr Leu Ile Arg Thr Ile Asn Gly Leu Vai 55 Arg Ala Thr Giu Gly Thr Val Thr Val Gly Pro His Gin Ile Asn Thr 70 75 Leu Lys Gly Lys Ala Leu Arg Asp Ala Arg Giy Gin Ile Gly Met Ile 90 Phe Gin Giy Phe Asn Leu Ser Giu Arg Ser Ser Val Phe Gin Asn Val 100 105 110 Leu Val Gly Arg Phe Aia His Thr Ala Trp Trp Arg Asn Leu Leu Gly 115 120 125 Phe Pro Thr Glu His Asp Lys Gin Ile Ala Phe His Ala Leu Glu Ser 130 135 140 Vai Gly Ilie Leu His Lys Vai Trp Thr Arg Ala Giy Ala Leu Ser Gly 145 150 155 160 Gly Gin Lys Gin Arg Val Ala Ile Ala Arg Ala Leu Ser Gin Asp Pro 165 170 175 Ser Val Met Leu Ala Asp Giu Pro Val Ala Ser Leu Asp Pro Pro Thr 180 185 190 Ala His Ser Val Met Arg Asp Leu Glu Asn Ilie Asn Asn Val Giu Giy 195 200 205 Leu Thr Val Leu Val Asn Leu His Leu Ile Asp Leu Ala Arg Gin Tyr 210 215 220 Thr Thr Arg Leu Vai Gly Leu Arg Ala Gly Lys Leu Val Tyr Asp Gly 225 230 235 240 Pro Ile Ser Giu Ala Thr Asp Lys Asp Phe Glu Ala Ilie Tyr Gly Arg 245 250 255 WO 01/00805 Pro Ile Gin Ala Lys Asp Leu Leu Gly Asp Arg Ala 260 265 <210> 387 <211> 969 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(946) <223> RXN01000 <400> 387 ctttctatgc ctacgcggat gtttccgtga tcattctgga aat PCT/IBOO/00926 :ctcatc qtggtgattg tcattgaagt aatctccaac gcacttcgaa agaggctggt atg agc acc tta acc Met Ser Thr Leu Thr tct cac Ser His aaa ctg Lys Leu ata gct Ile Ala ccc gcg Pro Ala tgg tcg Trp Ser atg gcg Met Ala gga atg Gly Met tta cgg Leu Arg gca att Ala Ile 135 ctc gca Leu Ala 150 gt a Val1 aat Asn a cg Thr tgg Trp ggc Gly ggt Gly gcc Ala ttc Phe cta Le u ccg Pro atc Ile ctc Le u cat His cgc Arg 75 gCC Ala cgc Arg gcg Ala act Thr a gc Ser att Ile gag Giu gag Giu ca g Gin tgc Cys gga Gly 110 cgt Arg ggc Gly ccc Pro gct Ala aat Asn atg Met atg Met 80 gt g Val1 tat T yr ttC Phe act Thr 1 ccg Pro gcg Ala gag Giu ttt Phe t gg T rp gtc Val1 t gg Trp ccc Pro cct Pro 145 gcg Ala ctg Le u ctt Le u agc Se r cgt Arg tct Ser ctg Le u gaa Giu 130 ttt Phe cgc Arg att Ile ccg Pro gat Asp tcc Ser gt C Val cg t Arg 115 gtg Val1 act Thr ccc Pro gt c ValI cag Gin ccc Pro atc Ile cct Pro 100 acc Th r gt t Val ggt Gly aac Asn ct t Le u atg Met ga t Asp gcc Ala ct g Le u gtt Val1 atc Ile g cg Al a 115 163 211 259 307 355 403 451 499 547 595 ttg ggt atc tcc ggt att gga caa cag gca aag tgg acc tat Leu Gly Ile Ser Gly Ile Gly Gin Gin Ala Lys Trp Thr Tyr 165 WO 01/00805 gaa gcc att gag tcc Giu Ala Ile Giu Ser 170 gcg ggt gga act acg Ala Gly Gly Thr Thr 185 gtt gcg cca tcc att Val Ala Pro Ser Ile 200 atc cgt acc tct gcg Ile Arg Thr Ser Ala 215 agt atg ctt gcc aat Ser Met Leu Ala Asn 230 atg ctg ctc atc gtc Met Leu Leu Ile Val 250 tcc ggc acc atc cgc Ser Gly Thr Ile Arg 265 gtg gca cca agc aac Val Ala Pro Ser Asn 280 ccc Pro gag Glu tct Ser t tg Le u 220 acc Th r gtt Val cgc a cc Th r gt t Val1 ttt Phe 205 99C Gly aac As n gtc ValI atc ggc Giy ctg Le u 190 gcc Ala atc Ile tac Tyr gca Ala a tg Met ccg tca Pro Ser 175 cgg tgg Arg Trp ctg tac Leu Tyr gtt ggt Val Giy agg cag Arg Gin 240 acg atg Thr Met 255 aag ggg Lys Gly gag Giu g cg Ala cgc Arg gca Ala 225 tgg Trp atc Ile gct Ala PCTIBOOIOO926 gca gtg cgt gca 643 Ala Val Arg Ala 180 ttg tgg cca cag 691 Leu Trp Pro Gin 195 ttt gag atc aac 739 Phe Giu Ile Asn 210 ggt ggt atc ggt 787 Gly Gly Ile Gly gac acc gtg ggc 835 Asp Thr Val Gly 245 gtc gat ctc atc 883 Val Asp Leu Ile 260 agt gac cgt gtc 931 Ser Asp Arg Val Arg Arg Ile 270 tgacgctcca ccaagcatcc gca <210> 388 <211> 282 <212> PRT <213> Corynebacterium glutamicum <400> 388 Met Ser Thr Leu Thr Ser His Arg Thr 1 5 Pro Ala Arg Pro Asn Lys Leu Ala Arg Ala Leu Ile Val Leu Ile Ala Thr Gly Giu Leu Pro Gin Met Pro Ala Gin Vai 55 Phe Ser Asp Pro Asp Trp Ser Lys Phe 70 Trp Arg Ser Ile Aia Met Aia Trp Leu Val Ser Val Pro Leu Gly Met Leu Aia 100 105 Trp Leu Arg Thr Val Leu Arg Phe Val ValI As n Th r Trp Gly Gi y Al a Phe Pro Ile Le u His Arg 75 Aila Arg Aila Aia Val Ly s T yr Al a Ile Gly Val1 Pro Al a As n Met Met Vali T yr Ph e WO 01/00805 WO 0100805PCTIiBOO/00926 Pro Pro 145 Ala Glu Ala Arg Ala 225 Trp Ile Al a Giu 130 Phe Lys Ala Le u Phe 210 Gly Asp Val1 Se r 115 Val1 Thr Trp Val1 Trp 195 Giu Gly Th r Asp Asp 275 Ile 135 Ala Ala Gly Al a Arg 215 Met Le u Gly Ala 120 Ile Leu Ile Gi y Pro 200 Th r Le u Leu Thr Pro 280 Leu Gly Gi u Thr 185 Se r Se r Al a Ile Ile 265 Ser Th r Ser 155 Thr Pro Al a Val1 Tyr 235 Val Arg Val1 140 Gly Pro Glu Ser Leu 220 Thr Val Arg Th r Gin 160 Se r T rp Tyr Gly Gin 240 Met Gly <210> 389 <211> 1173 <212> DNA <213> Corynebacteriun qiutamicum <220> <221> CDS <222> (1150) <223> RXN01732 <400> 389 gattotatog ctqatctco totocttgoc tqggtttgtt ctttcttgtc tcatgtcgct gaaaggtttt taaagatctc aag cca toc aag tco atg ogt Lys Pro Ser Lys Ser Met Arg aco ctt gct ota gtt gqt tgt Thr Leu Ala Leu Val Gly Cys tca tct gcg tot tot tct tcg Ser Ser Ala Ser Ser Ser Ser gt t Val1 too Ser ga t Asp 45 gtt tot Val Ser 15 too gat Ser Asp gca ago Ala Ser cocgggtctc ctcttcttaa atg ttc aag cto tot Met Phe Lys Leu Ser 1 acg ott gog ato tot Thr Leu Ala Ile Ser gag tot tot too toa Glu Ser Ser Ser Ser oag tgg oot gag too Gin Trp Pro Glu Ser WO 01/00805 ait act ttg Ile Thr Leu gcg tig gci Ala Leu Ala gtc aat ggt Val Asn Gly ggi gct gat Gly Ala Asp aac gcg att Asn Ala Ile 120 ttc ggt gcc Phe Gly Ala 135 aag tac tgc Lys Tyr Cys 150 gta gac atg Val Asp Met acc ggi gag Thr Gly Giu ggi gac aag Gly Asp Lys 200 tac cct atc Tyr Pro Ile 215 ttt qtt cag Phe Val Gin 230 cig aac ggt Leu Asn Gly acc gig cig Thr Val Leu cii Leu i ig Le u gig Val gc t Ala cag Gin i cg Ser gat Asp tac T yr 170 cca Pro gcg Al a gct Ala cca Pro gcg Ala 250 gag Glu gt Val1 act Thr 75 gcg Ala cag Gin tac Tyr tac Tyr gcc Ala 155 t gc Cys gca Al a ci g Le u aig Met gta Val1 235 gaa Glu gct Ala cct Pro 60 gai Asp ici Se r gig Val gat Asp ict Ser 140 cca Pro aac Asn gci Ala ca g Gin cag Gin 220 gag Glu gig Val1 cci Pro tci Ser iac Tyr ga t Asp a ic Ile gcg Ala 125 gci Al a gig Val1 ggi Gly cii Le u gci Ala 205 gati Asp ggC Gi y icc Se r aac Asn act Thr cig Leu tac Tyr atc Ile 110 ca g Gin gig Val1 gc i Al a at Ile gati Asp 190 gca Al a cig Le u aac Asn tic Phe g ca Ala 270 gag Glu ict Se r g ci Ala 95 act Thr cig Leu gcg Ala gcg Al a gaa Glu 175 gcg Al a acc Thr ggc Gi y aac As n gg t Gly 255 gci Al a ggi Gly gag Glu 80 gca Al a ga t Asp at Ile tac Tyr ici Se r 160 act Th r ct g Leu ici Se r aig Mei aac As n 240 tic Phe gag Glu gag Glu aac As n acc Thr gcg Ala ci g Le u acc Th r 145 tat Tyr gag Gi u gaa Glu cci Pro gat Asp 225 gct Ala tgg Trp gat Asp ga t Asp ct Leu gtt Val1 gg t Gly cgi Arg 130 aa c As n gct Ala ggc Gly aag Lys gcg Al a 210 a cc Th r gig Val1 ga t Asp gtc ValI ii g Le u ggi Gly gag Giu icc Ser 115 gati Asp aat As n gcg Ala cag Gin aic Ile 195 ggt Gly gat Asp cig Le u gcg Ala gia Val1 275 PCT/IBOO/00926 gct gaa 307 Ala Giu ati gag 355 Ile Glu gci tig 403 Ala Leu 100 cig tat 451 Leu Tyr gig cgt 499 Val Arg cci gat 547 Pro Asp icc gat 595 Ser Asp 165 gci gct 643 Ala Ala 180 gag icc 691 Glu Ser tac cag 739 Tyr Gin icc gct 787 Ser Ala ici gtc 835 Ser Val 245 cgi icc 883 Arg Ser 260 gcg itt 931 Ala Phe gcc tac acc gag aig aic cci aac Ala Tyr Thr Giu Met Ilie Pro Asn ggt ggc Gly Gly gic gca gcg icc aag icc Val Ala Ala Ser Lys Ser 290 WO 01/00805 ctt cca tcc gac ctg gtg gaa aag ctc Leu Pro Ser Asp Leu Val Glu Lys Leu 295 300 gca gat tcc tcc gag gaa gcc aag gat Ala Asp Ser Ser Giu Giu Ala Lys Asp 310 315 cta tct gac tgg act gct gat acc gca Leu Ser Asp Trp Thr Ala Asp Thr Ala 330 gqc gag atc ctg aag aag ttc tcc aac Gly Glu Ilie Leu Lys Lys Phe Ser Asn 345 350 ctc <210> 390 <211> 350 <212> PRT <213> Corynebacterium giutamicum <400> 390 Met Phe Lys Leu Ser Lys Pro Ser Lys PCT11B00100926 acc gag ttg atg gat gat tac 1027 Thr Glu Leu Met Asp Asp Tyr 305 gtc atg ttc gac atg gtt ggt 1075 Val Met Phe Asp Met Val Giy 320 325 cag gat gaa atc act cqt tac 1123 Gin Asp Giu Ile Thr Arg Tyr 335 340 taatttccct gtttccaata 1170 1173 Le u Ser Trp Asp Le u Val Giy Arg 130 Asn Al a Ser Ser Ser Giu Glu Le u T yr Arg Asp Asp 165 Le u Ser Ser Pro Val1 Gin 105 Giu Thr Asp Le u Ser 10 Val1 Se r Leu Leu Vali 90 Ala Gin Se r Asp T yr 170 Pro Met Gly Ser Val1 Thr Ala Gin Tyr Tyr Ala 155 Cys Ala Val1 Ser Asp Se r Tyr Asp Ile Al a 125 Ala Val1 Gly Leu Ala Se r Ala Thr Leu Tyr Ile 110 Gin Val1 Al a Ile Asp 190 Val Se r Al a Glu Se r Ala Th r Leu Al a Ala Glu 175 Ala Ser Asp Ser Gly Glu Ala Asp Ile T yr Ser 160 Thr Le u Giu Gly Gin Ala Ala Thr Gly Giu Gly WO 01/00805 Glu Lys Ile Glu Ser Gly Asp Lys Val Ala Leu Gin PCTIBOO/00926 Ala Ala Thr Ser 205 195 200 Ala 210 Thr Val Asp Val1 Al a 290 Met Asp Ile Gly Asp Leu.

Ala Val1 275 Ser Asp Met Thr I le Gln Gly Leu Thr 280 Ser Se r Asp Ile Val1 ValI Asp Se r 265 Gl u Asp Se r Trp Le u 345 Ala Pro Ala 250 Glu Met Leu Glu Th r 330 Lys Gin 220 Giu Val Pro Pro Glu 300 Ala Asp Phe Met Asn 240 Phe Giu Val1 Glu Met 320 Asp <210> 391 <211> 903 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(880) <223> RXN03080 <400> 391 cttgcaaaca ggcgiggtgg tqgcqitcat iggctcacca cagcatgcgc aagcgacgcg gattggggct gtaaaaactc gaa Giu aac Asn cca Pro gtt cg t Arg tct Ser ggt Gi y tcC gat Asp ttt Phe gct Ala acc ctc Leu ict Se r ggt Gly 9gC i ct Ser qic Val t cg Se r ggg atittcctii atttactqct atg cct caa tta gtt Met Pro Gin Leu Val 1 cgc cat gca gig aaa Arg His Ala Val Lys acc gca cig ati ggc Thr Ala Leu Ile Gly gcg att gca gga tig Ala Ile Ala Gly Leu agi ggg git gcg icg 115 163 211 259 307 WO 01/00805 PCT[IBOO/00926 Val Giu Ser Thr Gly Giu Val Met Vai Gly Gly Ser Gly Val Ala Ser aaa agc Lys Ser acc gag Thr Glu cgc tac Arg Tyr cgc qcc Arg Ala gag cag Giu Gin 135 atc ggc Ile Gly 150 ccc gtc Pro Val ctg cgc Leu Arg gat ctc Asp Leu gac ggc Asp Gly 215 gcc aca Ala Thr 230 ccc gaa Pro Glu 60 gct aaa gcc Ala Lys Ala ttg cgc att Leu Arg Ile ccg cat cgt Pro His Arg 105 acc gat gac Thr Asp Asp 120 ccc gtc aac Pro Val Asn cga gcg ctc Arg Ala Leu tcc gcc ctt Ser Ala Leu 170 gcc cga gct Ala Arg Ala 185 aac cac gtt Asn His Val 200 gaa qtt gtc Glu Val Val ctg tcc acc Leu Ser Thr acc agc tca Thr Ser Ser cga Arg 75 ggt Gly ggc Gly gcc Ala gaa Giu gcc Ala 155 ga t Asp aat Asn gcc Ala t cc Ser gtg Val 235 ctt Le u gcc cgc Ala Arg ttt agt Phe Ser cgc ttc Arg Phe ctg cgc Leu Arg 125 tta tcg Leu Ser 140 caa gac Gin Asp cta. cgg Leu Arg tcc ggc Ser Gly cgt tgg Arg Trp 205 caa ggt Gin Gly 220 tac gga Tyr Gly cgc gtg Arg Val gtg Val gtc Val1 ga c Asp ctc Leu 130 cag Gin ctt Leu gt c Val gtc Val1 gtg Val 210 gtg Val1 gtg Val c cg Pro gcg Al a g ca Ala 115 gac Asp ctc Leu ctc Le u ctt Leu gtc Val1 195 ttg Le u ctc Le u cg c Arg caa Gin atg Met 100 gat Asp atc Ile atc Ile ga c Asp caa Gin 180 ctt Le u atg Met ga a Glu ga t Asp aac As n ggc GI y cga Arg g cc Ala cac His gag Giu 165 ct c Leu cac His gcc Ala cct Pro ga t Asp 245 atc ccg cat cca aat ccc ttt Ile Pro His Pro Asn Pro Phe 255 260 880 tgattgaaag tttgacttaa aaa <210> 392 <211> 260 '212> PRT <213> Corynebacterium glutamicum <400> 392 Met Pro Gin Leu Val Giu Ile Arg Asp Leu Asn Val Glu Phe Pro Ser WO 01/00805 1 Arg His A Thr Ala L Ala Ilie A Ser Gly V Leu Val P Val Val A Thr Asp A 1 Ala Leu A 130 Gin Gin L 145 Val Leu L Glu Val L Ile Val V 1 Ala Val L 210 Glu Vai L 225 Ala Val A Pro Asn P 5 Val Lys Ile Giy Gly Leu Ala Ser Gin Asn Met Giy 100 Asp Arq Ile Ala Ile His Asp Giu 165 Gin Leu 180 Leu His Met Ala Glu Pro Asp Asp 245 Phe 260 As n Pro Val1 Lys 70 Thr Arcj Arg Glu Ile 150 Pro Le u Asp Asp Al a 230 Val1 Asn Glu Ser Giu Tyr Ala Gin 135 Gly Val1 Arg Le u Gly 215 Th r 10 Ser Ala Gly Lys Gly Giu Ala Arg 75 Ilie Gly 90 Arq Gly Asp Ala Asn Glu Leu Ala 155 Leu Asp 170 Ala Asn Val Ala Val Ser Thr Val 235 Ala Thr Met Arg Ser Phe Arg 125 Ser Asp Arg Gly Trp 205 Gly Gly Gly Ala Val Leu Ala Al a 110 Ala Gly Th r His Th r 190 Cys Asp Leu PCTJIBOO/00926 Lys Val Leu Ser Gly Gly Leu Ser Arg Asp Val Giu Ile Asn Gly Gin Ala Val 160 Gin Val 175 Thr Val Asp His Ile Arg Pro Ile 240 Pro Glu Thr Ser Ser Leu Arg Val Ile Pro His <210> 393 <211> 582 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(559) <223> RXN03081 WO 01/00805 <400> 393 acggactgcc cattgcggtg cgcgatgatc ccgaaaccag PCTIBOO/00926 ctcacttcgc gtgatcccgc atccaaatcc cttttgattg aaagtttgac ttaaaaaccc atg aaa aaa tca ctc Met Lys Lys Ser Leu att Ile gac Asp agc Se r ga t Asp acc Thr gtc Val1 ct g Le u ccc Pro 120 cqt Arg gtt Val tct Ser gtt Val t cc Se r cca Pro 9 tg Val gcc Ala 105 gaa Glu ttc Phe ctc gtg Leu Val tcc gga Ser Gly gac ggc Asp Gly 45 tta gaa Leu Glu tcc ttg Ser Leu qaa atg Glu Met gaa tac Glu Tyr 110 gcc gqc Ala Gly 125 caa ggc Gin Gly 1 tta agc ggc Leu Ser Gly act gtg qaa Thr Val Glu gca ttc cca Ala Phe Pro tcc aaa cca Ser Lys Pro gtt ctc ccc Val Leu Pro gac acc gct Asp Thr Ala caa gtc aaa Gin Val Lys gac cca gat Asp Pro Asp acc atc gac Thr Ile Asp 145 tgc C ys acc Thr cgc Arg gag Glu atc, Ile gac Asp aac Asn ttg Le u 130 att Ile tct Ser t cg Se r gtc Val1 atc Ile Gly ga a Giu 100 ggc Gly atc: Ile gaa Gi u gat Asp att Ile a ca Thr gcc Ala gcc Ala acc Thr agg Arg gtc ValI Gi y 115 163 211 259 307 355 403 451 499 547 Asp Thr Giu 140 ctc aac gtc ccg tagttaactt cgattcaqac gct Leu Asn Val Pro 150 <210> 394 <211> 153 <212> PRT <213> Corynebacterium glutamicum <400> 394 Met Lys Lys Ser Leu Ilie Ala Ile Val Ala Ser Ala Leu Val Leu Ser 1 5 10 Gly Cys Thr Ser Asp Ser Ser Asp Ser Ser Gly Thr Ser Gly Thr Val 25 Glu Thr Thr Ser Ile Thr Thr Ser Val Ala Ala Ala Asp Gly Ala Phe WO 01/00805 40 Pro Arg Thr Val Thr Leu Asp Asp Ser Ser Ile 55 Pro Giu Arg Ile Ala Val Leu Thr Pro Giu Ala 70 Pro Ile Thr Gly Ala Asp Arg Val Val Met Thr Ala Asp Giu Glu Thr Ala Ala Leu Ala Ser Gin 100 105 Lys Asn Gly Gly Arg Leu Asp Pro Giu Gin Val 115 120 Asp Leu Val Ile Val Ser Ala Arg Phe Asp Thr 130 135 Asp Ile Leu Giu Gly Leu Asn Val Pro 145 150 <210> 395 <211> 444 <212> DNA <213> Corynebacterium qiutarnicum <220> <221> CDS <222> (421) <223> RXN03082 <400> 395 cagaagcaat cgcagaaatc gatgcaaacc gcattcgaca ccccactgtg ctcactttga tgcaacgcgg accacgccaa PCT/IBOO/00926 Thr Ala Ala ValI Val Giu 140 Leu Ser Giu Giu Ala 125 Gin tct Se r gta Val1 gt t Val aaa Lys aac As n acc Thr 99C Giy gca Ala aac Asn att Ile ttcgacaagc ctgccacctc atg gtc atg cca gaa Met Val Met Pro Glu 1 ggc ggc act cca gtg Gly Gly Thr Pro Val gca gac cca gaa caa Ala Asp Pro Glu Gin cag gac ttc caa ggt Gin Asp Phe Gin Gly aac cca gcg cta gcc Asn Pro Ala Leu Ala tac gcc gac act gtc Tyr Ala Asp Thr Val WO 01/00805 PCTIIBOO/00926 acc act gga gtt act gca ggt acc gat atc acc act ggt ctg cag caa 403 Thr Thr Gly Val Thr Ala Gly Thr Asp Ile Thr Thr Gly Leu Gin Gin 95 100 gtg gca gaa atg ctg agc tagttttgaq atgttgaaac tag 444 Vai Ala Giu Met Leu Ser 105 <210> 396 <211> 107 <212> PRT <213> Corynebacterium giutarnicum <400> 396 Met Val Met Pro Giu Ser Aia Met Leu Thr Giy Leu Ile Arg Giu Aia 1 5 10 Giy Gly Thr Pro Val Val Asp Ser Leu Giy Ala Val Gly Thr Ile Thr 25 Ala Asp Pro Giu Gin Val Vai Ala Met Aia Pro Giu Ile Ile Ile Ile 40 Gin Asp Phe Gin Giy Lys Giy Arg Giu Asn Phe Aia Asn Phe Leu Ser 55 Asn Pro Ala Leu .Aia Asn Vai Pro Ala Ile Giu Asn Asp Lys Ile Phe 70 75 Tyr Ala Asp Thr Val Thr Thr Gly Vai Thr Aia Giy Thr Asp Ile Thr 90 Thr Giy Leu Gin Gin Val Aia Giu Met Leu Ser 100 105 <210> 397 <211> 1479 <212> DNA <213> Corynebacteriun giutamicun <2 <221> CDS <222> (101)..(1456) <223> RXA02261 <400> 397 gaaatctata gaacgatagg taaaaactgg actaggttta tctatagcgg aatagaaaat actccgctcg acagcatcac ttagctgaaa ggcctttaac atg qac ccc tca gat 115 Met Asp Pro Ser Asp 1 cta gcc tgg att ctc gca gct ttt gcg ttg gta agc ctg atg ttc ccc 163 Leu Ala Trp Ile Leu Ala Ala Phe Ala Leu Val Ser Leu Met Phe Pro 15 gga ttg tcc ctg ctc tac ggc ggc atg ctg ggt ggg caa cac gtt ctt 211 Giy Leu Ser Leu Leu Tyr Gly Gly Met Leu Gly Giy Gin His Val Leu 30 WO 01/00805 aac acg ttc Asn Thr Phe tac atc att Tyr Ile Ile tgg gga att Trp Gly Ile atg gaa gat Met Glu Asp ctg ttc gct Leu Phe Ala cgc atg cgc Arg Met Arg 120 ttt gtg tac Phe Val Tyr 135 gag tcc ggc Glu Ser Gly 150 gac ttt gct Asp Phe Ala ctc gcq ctg Leu Ala Leu cca cac aac Pro His Asn 200 ggc tgg ttc Gly Trp Phe 215 gca agc tac Ala Ser Tyr 230 atg gqc ttc Met Gly Phe a tgc Met gga Gly gg a Gi y gg C Gly atc Ile gga Gi y cca Pro gtg Val1 gga.

Gly 170 ata Ile cca Pro ttC Phe gtc Val1 ctc Leu 250 gtt Val1 cac His aat As n 75 acc Th r t ca Ser gcg Al a ct g Le u gg t Gi y 155 a cg Th r gtg Val ctg Leu aa t As n gtt Val1 235 gtt Val1 a tg Met gga Gly

CCC

Pro g ga Gly ctc Le u tgg Trp gca Al a 140 gg C Gly gca Ala ct g Leu att Ile ggt GI y 220 acc Th r ga a Gi u agc Ser ctt Le u ct t Le u ga c Asp gca Al a ctg Le u 125 cac His t gg Trp gtg ValI gga Gly t tg Le U 205 ggt Gly t ct Se r cgt Arg t ca Ser gtc Val1 gaa Glu ctc Le u ctt Le u 110 gtc Val t gg Trp atq Met cac His cg c Arg 190 att Ile acc Thr ct C Le u gtg Val ctt Leu tta Leu tac Tyr a tg Met 95 gtt Val1 ttC Phe gtt Val aaa Lys a tg Met 175 cgc Arg ggt Gly g ca Ala att Ile ttc Phe 255 agc Ser atc Ile cg C Arg ttC Ph e gca Ala 115 tgg Trp ga t Asp gag Glu g cg Ala gct Al a 195 a tc Ile aac As n ggC Gly

CCC

Pro PCTIIBOO/00926 ctt gtg 259 Leu Val ggt ggg 307 Gly Gly aac att 355 Asn Ile tac att 403 Tyr Ile 100 gcg ggg 451 Ala Gly ttc acc 499 Phe Thr gat cct 547 Asp Pro ttc cac 595 Phe His 165 tct gga 643 Ser Gly 180 gtg cgt 691 Val Arg gtt gcg 739 Val Ala ttc ctc 787 Phe Leu gga. atg 835 Gly Met 245 act ttc 883 Thr Phe 260 ttt ggc tcg gca acc ggc aca atc gca ggc ctt Phe Gly Ser Ala Thr Gly Thr Ile Ala Gly Leu gtg gct Val Ala atc acc ccg Ile Thr Pro WO 01/00805 gcc gcg gat Ala Ala Asp 280 ggc gca gtt Gly Ala Val 295 gtc gat gat Val Asp Asp 310 gca ggt gca Ala Gly Ala gga gtt tcc Gly Val Ser gaa cca ctg Giu Pro Leu 360 tgg ttg att Trp Leu Ile 375 .tcc gaa gcc Ser Giu Ala 390 gcc tac cac Ala Tyr His ttc gga cct Phe Gly Pro ggc gtc gat Gly Val Asp 440 gca Ala gt c Val1 t cc Se r ct g Le u gga Gly 345 gcc Ala gcc Al a ga a Gl u ct C Le u ga a Giu 425 aag a gc Se r ttc Ph e ga t Asp 315 gtc Val1 ttC Phe atc Ile atc Ile g aa Glu 395 tcC Ser ccc Pro aaa ccg Pro tgg T rp 300 gtg Val atg Met ttc Phe gtg Val1 ttg Leu 380 ggc Gly aac As n gag Glu atc gga Gly att Ile gca Ala ttt Phe ggc GI y 350 ct t Le u aa g Lys ga c Asp att Ile acc Thr 430 ttC Ph e tgg Trp cac His 320 ga t Asp ctc Le u tac Tyr atg Met gca Ala 400 atg Met ccc Pro gcc Al a aag Ly s 305 gga Gly cca Pro t cC Se r g ca Ala act Thr 385 ga a Glu gca Al a gac Asp PCTIBOO/00926 gcg ctc 979 Ala Leu cac cga 1027 His Arg ggc att 1075 Giy Ile 325 cca gcg 1123 Pro Ala 340 tgg agg 1171 Trp Arg gtg acc 1219 Val Thr atc acc 1267 Ile Thr gaa tct 1315 Giu Ser 405 acc aat 1363 Thr Asn 420 cag gtg 1411 Gin Val gct gat-act cga aag gcc tca aaa Ala Asp Thr Arg Lys Ala Ser Lys Lys Gin Lys Ile 1456 1479 tgaccgcaac ctacaccact gaa <210> 398 <211> 452 <212> PRT <213> Corynebacterium glutamicum <400> 398 Met Asp Pro Ser Asp Leu Ala Trp Ile Leu Ala Ala Phe Ala Leu Val 1 5 10 Ser Leu Met Phe Pro Gly Leu Ser Leu Leu Tyr Gly Gly Met Leu Gly 25 Gly Gin His Val Leu Asn Thr Phe Met Met Val Met Ser Ser Leu Gly 40 WO 01/00805 PCTIIBOO/00926 Ile Ile Ser Leu Val Tyr Ile Ile Tyr Gly His Gly Leu Val Leu Gly 55 Asn Ser Ile Gly Gly Trp Gly Ile Ile Gly Asn Pro Leu Glu Tyr Phe 70 75 Gly Phe Arg Asn Ile Met Glu Asp Asp Gly Thr Gly Asp Leu Met Trp 90 Ala Gly Phe Tyr Ile Leu Phe Ala Ala Ile Ser Leu Ala Leu Val Ser 100 105 110 Ser Gly Ala Ala Gly Arg Met Arg Phe Gly Ala Trp Leu Val Phe Gly 115 120 125 Val Leu Trp Phe Thr Phe Val Tyr Ala Pro Leu Ala His Trp Val Phe 130 135 140 Ala Ile Asp Asp Pro Glu Ser Gly Tyr Val Gly Gly Trp Met Lys Asn 145 150 155 160 Val Leu Glu Phe His Asp Phe Ala Gly Gly Thr Ala Val His Met Asn 165 170 175 Ala Gly Ala Ser Gly Leu Ala Leu Ala Ile Val Leu Gly Arg Arg His 180 185 190 Ser Met Ala Val Arg Pro His Asn Leu Pro Leu Ile Leu Ile Gly Ala 195 200 205 Gly Leu Ile Val Ala Gly Trp Phe Gly Phe Asn Gly Gly Thr Ala Gly 210 215 220 Gly Ala Asn Phe Leu Ala Ser Tyr Val Val Val Thr Ser Leu Ile Ala 225 230 235 240 Ala Ala Gly Gly Met Met Gly Phe Met Leu Val Glu Arg Val Phe Ser 245 250 255 Gly Lys Pro Thr Phe Phe Gly Ser Ala Thr Gly Thr Ile Ala Gly Leu 260 265 270 Val Ala Ile Thr Pro Ala Ala Asp Ala Val Ser Pro Leu Gly Ala Phe 275 280 285 Ala Val Gly Ala Leu Gly Ala Val Val Ser Phe Trp Ala Ile Ser Trp 290 295 300 Lys Lys Gly His Arg Val Asp Asp Ser Phe Asp Val Phe Ala Val His 305 310 315 320 Gly Met Ala Gly Ile Ala Gly Ala Leu Phe Val Met Leu Phe Gly Asp 325 330 335 Pro Leu Ala Pro Ala Gly Val Ser Gly Val Phe Phe Gly Gly Glu Leu 340 345 350 Ser Leu Leu Trp Arg Glu Pro Leu Ala Ile Ile Val Thr Leu Thr Tyr 355 360 365 WO 01/00805 Ala Phe Gly Val Thr Trp Leu Ile Ala Thr Ile 370 375 Thr Leu Arg Ile Thr Ser Giu Ala Giu Tyr Giu 385 390 395 Giu His Ala Giu Ser Ala Tyr His Leu Asn Ser 405 410 Ala Thr Arg Thr Asn Phe Gly Pro Giu Ile Pro 420 425 Asp Ala Val Gin Val Gly Val Asp Lys Gin Lys 435 440 Lys Aia Ser Lys 450 <210> 399 <211> 1111 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1111) <223> RXA02020 <400> 399 ttggtcaatc aaqcqtgaat ccggcttcca tgagccagtt Leu 380 Gly Asn Giu Ile Asn Lys Ile Asp Gly Ile Giu Thr 430 Ala Asp 445 PCT(1B00100926 Phe Met Arg Ala 400 Gly Met 415 Val Pro Thr Arg gcccqcctca aaqcttgacc cattttcata accagtgcca tgtgggttta cggttgatac atg gct Met Ala aaa tct aat Lys Ser Asn qaa Giu ctc Le u atc Ile gcc Al a cgt Arg cac His atg 99g Gly ggC Gly cgc Arg atc Ile ccc Pro t gg T rp gtg qga Gly g ca Ala gcc Ala gtg Val tcc Ser 99 t Gly 99C acc Th r att Ile Gly ct t Le u gga Gly ttc Phe gcc gga Gly gg t Gly ccc Pro gtt Val1 tcg Se r 75 tcc Se r ga a acc Thr ct g Le u 30 CtC Le u a tg Met cgt Arg t gg T rp ggC cac His ctc Le u gcg Al a ggC Gly 9gC Gly tac Tyr gct 1 ctc Leu ggc Gly tac Tyr gag Giu gag Giu t 99 Trp gcc a ca Thr acc Th r atc Ile atg Met ga t Asp ttc Phe atc atg Met ggc Gi y atc Ile gct Ala gct Aila atg Met atg atg Met gt t Val1 9CC Al a gct Ala ttc Phe ctg Le u 100 ggt 115 163 211 259 307 355 403 451 WO 01/00805 Met Val Met tgg ttc ggC Trp Phe Gly 120 ttc gct gtg Phe Ala Val 135 tac tgg ttc Tyr Trp Phe 150 att ggt att Ile Gly Ilie gga acc tca Gly Thr Ser tct ggt gtt Ser Gly Val 200 att gaa att Ile Glu Ilie 215 gct att tcc Ala Ilie Ser 230 tac ttg ggc Tyr Leu Gly atc aat ggt Ile Asn Gly gcg atg gca Ala Met Ala 280 gtt cta gca Val Leu Ala 295 cgt ttg gta Arq Leu Val 310 agt aag ctc Ser Lys Leu Gly 105 gtc ValI gtg Val1 gca Al a gct Ala aac As n 185 gct Al a gtC ValI c tg Le u t Ct Se r gcc Al a 265 aac Asn ctg Le u ttt Phe Al a gaa Glu aac Asn ttc Phe ctt Leu 170 ttc Phe gct Ala acc Thr g cg Ala gtt Val1 250 gac Asp atc Ile ctt Le u tcC Ser Glu ccg Pro ctc Le u att Ile 155 att Ile atc Ile ggt Gly att Ilie gtg ValI 235 t tg Le u acc Thr cct Pro tcc Se r atg Met 315 Met tgg Trp gtc Val 140 aag Ly s ttc Phe ggt Gly ttg Le u gca Ala 220 cg t Arg gt C ValI gct Ala ggc Gly gct Al a 300 gcg Al a Thr att Ile 125 gcg Ala gtc Val gg a Gly gat Asp ctc Le u 205 gc t Al a gcc Ala atc Ile gcg Ala a cg Thr 285 ttc Phe aat Asn g tc Val1 Ala tcg Se r cg C Arg gtg Val1 ctt Leu 175 qga Gi y gtg Val1 gag Glu att Ile ttc Phe 255 t CC Ser g gt Gly gcc Ala caa Gln Ala ctt Leu gg t Gi y atc Ile 160

CCC

Pro ttc Phe g ct Al a tcc Ser tgg Trp 240 CtC Leu

CCC

Pro ttc Phe caa Gin gac Asp 320 Ala gtc ValI ttc Phe 145 atc Ile gga Gly atg Met ttt Phe gat Asp 225 cgt Arq atg Met ttc Phe atg Met atc Ile 305 gct Al a g cg Al a Ile tgC Cys 130 gg t Gly gct Ala tcC Se r

CCC

Pro gcc Ala 210 aag Lys att Ile cct Pro acc Th r ga a Giu 290 tat T yr C cg Pro Met 115 gtg Val1 gaa Glu ttc Phe acc Thr aat Asn 195 ttt Phe cca Pro t ca Se r tat Tyr caa Gin 275 gcg Ala gcc Ala cga Arg PCTIIBOO/00926 Gly Ala gtC ttc 499 Val Phe ttc gag 547 Phe Glu ctc atc 595 Leu Ile 165 ttt gtt 643 Phe Val 180 ggt att 691 Gly Ile ggt ggc 739 Gly Gly cgt gaa 787 Arg Glu gtc ttt 835 Val Phe 245 gag tcg 883 Glu Ser 260 atc ctg 931 Ile Leu atc atc 979 Ile Ilie act tct 1027 Thr Ser gtt ttC 1075 Val Phe 325 agc acc agc cac Ser Thr Ser His 330 ccc acc aat Pro Thr Asn 1111 <210> 400 WO 01/00805 <211> 337 <212> PRT <213> Corynebacterium glutamicum <400> 400 Met Ala Lys Ser Asn Glu Gly Leu Gly PCT[IBOO/00926 1 Le u Gly T yr Glu Giu Trp Ala Val1 Phe 145 Ile Gly Met Phe Asp 225 Arg Met Phe Met Met Val1 Ala Ala Phe Le u 100 Gly Val1 Phe Le u Phe 180 Gly Gly Arg Val Glu 260 Ile Ile 5 Gly Gly Gly Ala Gly Ile Ala Phe Glu Ilie 165 Val1 Ile Gly Giu Phe 245 Ser Leu Ile Gly Arg Ilie Pro Trp Val1 Phe Ala 135 Trp Gly Thr Gly Glu 215 Ile Leu Asn Met Le u 295 Se r Ala Val1 Al a Ala Met Gly 120 Val1 Phe Ile Se r Val1 200 Ile Ser Gly Gly Al a 280 Ala Th r 10 Ile Gly Leu Gly Phe 90 Ala Glu Asn Phe Leu 170 Phe Ala Thr Ala Val 250 Asp Ile Le u Gly Gly Pro Val Ser 75 Ser Glu Pro Le u Ilie 155 Ile Ile Gly Ile Val1 235 Leu Th r Pro Se r Le u Ala Ala Met Phe Le u Met T rp Val 140 Lys Phe Gly Leu Ala 220 Arg Val Ala Gly Ala 300 Arg Gly Val Gin Ser Gly Thr Ile 125 Ala Val1 Giy Asp Leu 205 Ala Ala Ile Ala Thr 285 Phe Th r Leu Le u Met Arg Trp Gly 110 Pro Val1 Ala Trp His 190 Ala Ala Val1 Thr Glu 270 Val1 Asn Arg Phe Leu Leu T yr Le u Ala Ser Arg Val1 Leu 175 Gly ValI Glu Ile Ph e 255 Se r Gly Ala His Leu Ala Gly Gly Tyr Ala Leu Gly Ile 160 Pro Phe Ala Se r Trp 240 Le u Pro Phe Gin WO 01/00805 PCTIiBOOIO0926 Ile Tyr Ala Thr Ser Arg Leu Val Phe Ser Met Ala Asn Arg Gin Asp 305 310 315 320 Ala Pro Arg Val Phe Ser Lys Leu Ser Thr Ser His Val Pro Thr Asn 325 330 335 Ala <210> 401 <211> 784 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(784) <223> RXA00281 <400> 401 ggttgactct ttggctttga tgccagcatt cgccaaagcg tggccctctt taagaaagct a act gaacaca acaqagtagg tttatgcgac actggtgcgc atg atc aac gtt gaa gqc Gly ttc Phe gcc Al a act Thr aat As n cca Pro 9 9g Gi y act Th r caa ca a Gin ccc Pro acc Thr gcc Al a aa a Lys gca Ala aaa Lys tca Se r ctt gca Ala ttt Ph e ctt Le u ccc Pro ga t Asp 80 tgg Trp ctc Le u ttt Phe 99c Ile Asn gat gat Asp Asp qqc ccc Gly Pro tta gat Leu Asp cga tcg Arg Ser aaa gca Lys Ala gCC cgt Ala Arg ctc gtg Leu Val 115 cta ggc Leu Gly 130 ccg gaa Glu a gc Ser ggc Gly cca Pro aaa Lys cac His aat Asn ct g Le u ggc Gly tta WO 01/00805 Gin Arg Leu Gly Leu Ala Ala Ala Leu 135 140 att ctc gac gaa ccc gtc aac ggc ctt Ile Leu Asp Glu Pro Val Asn Gly Leu 150 155 gtg cgc acc ttg ttg caa aac atc gcc Val Arg Thr Leu Leo Gin Asn Ile Ala 170 gtg agt tcc cac ctg ctg tcc gag atg Val Ser Ser His Leo Leu Ser Glu Met 185 190 atc gtg att ggg cgt ggc aag ctg gtc Ile Val Ile Gly Arg Gly Lys Leu Val 200 205 ttt gtg cgc tcc cat tcc gct tcc aca Phe Val Arg Ser His Ser Ala Ser Thr 215 220 <210> 402 <211> 228 <212> PRT <213> Corynebacterium giutarnicom <400> 402 Met Ilie Asn Val Giu Gly Leu Thr Lys 1 5 Val Asp Asp Leu Ser Phe Giu Val Lys 25 Leu Gly Pro Asn Gly Ala Gly Lys Ser 40 Gly Leo Asp Asn Pro Thr Ala Gly His' 55 Tyr Arg Ser Leo Lys Asn Pro Leu Thr 70 Ala Lys Ala Thr His Pro Asn Arg Thr Ile Ala Arg Ala Asn.Gly Leu Ser Thr 100 105 Thr Leu Val Gly Leu Thr Gly Val Gly 115 120 Ser Leo Gly Met Gly Gin Arg Leu Gly 130 135 Asp Pro Giu Tyr Leu Ile Leu Asp Glu 145 150 Glu Gly Ile His Trp Val Arg Thr Leu Leo ga c Asp aag Ly s 175 g cg Ala g cc Ala gtt Val Gin 10 Pro Thr Ala Lys Ala 90 Lys Se r Leu Pro Leu Gly cca Pro 160 ca g Gin ca a Gin ga t Asp gtg Val Tyr Gly Th r Thr Val 75 Glu Arq Lys Ala ValI 155 Gin Asp 145 gaa Glu gg c Gly act Thr a tg Met gtg Val 225 Gly Ile Met Ile Gly As n ValI Lys Al a 140 Asn As n Pro gg c Giy aga Arg gcg Al a ccc Pro 210 C99 Arg Gin ValI Arg Giu Ala His Asp Thr 125 Ala Gly Ile Giu att Ile acc Thr ga a Glu 195 atg Met gca Al a ValI Thr Leo Gi y Leu Leu Glu 110 Gly Leu Leu Ala

P

Tyr cac His gtg Val1 180 cat His cat His qca Ala Arg Gly Ile Gin Leo L ys Val1 Gly Leo Asp Lys CT11B00100926 Leo tgg 595 Trp 165 ctc 643 Leo ttg 691 Leo gag 739 Glu Ala Phe Leu Pro Asp Trp Leu Phe Gly Pro 160 Gin WO 01/00805 PCT/IBOOIOO926 165 170 175 Gly Arg Thr Val Leu Val Ser Ser His Leu Leu Ser Giu Met Ala Gin 180 185 190 Thr Ala Giu His Leu Ilie Val Ile Gly Arg Gly Lys Leu Vai Ala Asp 195 200 205 Met Pro Met His Giu Phe Val Arg Ser His Ser Ala Ser Thr Val Val 210 215 220 Val Arg Ala Ala 225 <210> 403 <211> 852 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (852) <223> RXN00570 <400> 403 acg aga cct cgg ccc Thr Arg Pro Arg Pro 1 5 ttc tcc gca tcc gga Phe Ser Ala Ser Gly gga aac ctt tcg cct Gly Asn Leu Ser Pro tta ggc aac gga ctg Leu Giy Asn Gly Leu ccg cag gca tac ttc Pro Gin Ala Tyr Phe tca ctt acc cac tta Ser Leu Thr His Leu acc ggc gtt gtg gtg Thr Giy Val Vai Val 100 atc atg gcg gta ctg Ilie Met Ala Val Leu 115 att ttc ctc cgc ttc Ile Phe Leu Arg Phe 130 caa gaa att ggc aac ggg Gin Glu Ile Gly Asn Gly ccc atc gca gtg atc ctg Pro Ilie Ala Vai Ilie Leu 25 gat caa aca tct tca tgg Asp Gin Thr Ser Ser Trp 40 ctc acg ctg tgg ctt acc Leu Thr Leu Trp Leu Thr tgg acg att ccc gga acc Trp Thr Ile Pro Gly Thr 70 75 agt ttc gct gaa gtt atc Ser Phe Ala Giu Val Ile 90 ttt gcg ctc gga tgg acc Phe Ala Leu Gly Trp Thr 105 cca cca acc atc gtg atg Pro Pro Thr Ilie Val Met 120 ctt gtc gca ctg att Leu Val Ala Leu Ilie gct Aia ttc Phe atg Met atc Ile gca Aia ctc Leu atg Met 125 gct Aia gga Gly tac Tyr gtg Val tac Tyr atc Ile 110 gt c Val1 gca Ala gca Ala cgc Arg 99 C Gly ct t Leu gg a Gly gca.

Ala g9 Ala ttt Phe agc Ser gac Asp gtt Val1 C99 Arg 99C Gly 48 96 144 192 240 288 336 384 432 qga ctc gac Gly Leu Asp 135 ctc atc gac Leu Ile Asp 9CC agc gtg acc gac Ala Ser Val Thr Asp 140 WO 01/00805 ccg ctc att Pro Leu Ile 145 agt ccc cgc Ser Pro Arg gtg gga acc Val Gly Thr cta gac aac Leu Asp Asn 195 tcc ttc gcc Ser Phe Ala 210 gtc att gtc Val Ile Val 225 cac cgc ccc His Arg Pro cta ccc atg Leu Pro Met acc aac gcg Thr Asn Ala 275 ccc Pro 150 agc Ser gcc Al a atc Ile atg Met qg C Gly 230 aac As n atc Ile gt c Val at g Met atc Ile atc Ile tcc Ser gaa Glu 215 caa Gin ctt Leu 99C Gly g cc Ala gtc ata gtt ttt gtg Val Ile Val Phe Val 155 gcc cca ccc gtt gca Ala Pro Pro Val Ala 170 gca tcc ggc aaa cta Ala Ser Gly Lys Leu 185 cgc ccc gtc ttt acc Arg Pro Val Phe Thr 200 ctc gtt gtt ccc ttg Leu Val Val Pro Leu 220 ggc gtc gca gtg ctt Gly Val Ala Val Leu 235 gcc gcc gcg gcc tcc Ala Ala Ala Ala Ser 250 aac atc acc acc tgc Asn Ile Thr Thr Cys 265 gga gca aaa tca cac Gly Ala Lys Ser His 280 PCTJIBOO/00926 gca ttg agc atg 480 Ala Leu Ser Met 160 gta gcc gca gta 528 Val Ala Ala Val 175 gcg tcc gga att 576 Ala Ser Gly Ile 190 gcc cca gaa ttt 624 Ala Pro Glu Phe 205 gcg atc acc gta 672 Ala Ile Thr Val aaa gca gca ggt 720 Lys Ala Ala Gly 240 gga ctg tgg tcc 768 Gly Leu Trp Ser 255 ctc acc ggc ccc 816 Leu Thr Gly Pro <210> 404 <211> 284 <212> PRT <213> Corynebacterium glutamicum <400> 404 Thr Arg Pro Arq Pro Gin Giu Ile 1 Phe Ser Ala Ser Gly Pro Ilie Ala Gly Asn Leu Ser Pro Asp Gin Thr 40 Leu Gly Asn Gly Leu Leu Thr Leu 55 Pro Gin Ala Tyr Phe Trp Thr Ilie Ser Leu Thr His Leu Ser Phe Ala Le u Al a Ile Tyr Val1 Gly Val1 Ala Phe Met Ilie Al a WO 01/00805 Thr Gly Val Ile Met Ala .115 Ile Phe Leu 130 Pro Leu Ilie 145 Ser Pro Arg Val Gly Thr Leu Asp Asn 195 Ser Phe Ala 210 Val Ilie Val 225 His Arg Pro Leu Pro Met Thr Asn Ala 275 <210> 405 <211> 498 <212> DNA Val 100 Val1 Arg Ala Leu Ile 180 Gly Ala Gin Gly Ala 260 ValI Le u Phe Leu Ala 165 Val Ilie Ile Asn Val 245 Leu Phe Pro Gly Pro 150 Ser Ala Ile Met Gly 230 Asn Ile Leu Met 125 Se r Al a Val Al a Al a 205 Al a Lys Gly Leu Ile 110 ValI Val Le u Ala Ser 190 Pro Ile Al a Leu Thr 270 PCTIBOO/00926 Gly Arg Ala Gly Thr Asp Ser Met 160 Ala Val 175 Gly Ile Glu Phe Thr Val Ala Gly 240 Trp Ser 255 Gly Pro Leu Ile Val Ala Gly Ala Lys Ser His <213> Corynebacterium glutamicum <220> <221> CDS <222> <223> FRXA00570 (400> 405 cca acc atc gtg atg gcc atg gtc gca ggc att ttc ctc cgc ttc gga Pro Thr Ile Val Met Ala Met Val Ala Gly Ile Phe Leu Arg Phe Gly 1 5 10 ctc gac ctc atc gac gcc agc gtg acc gac ccg ctc att gca ctt ccc Leu Asp Leu Ile Asp Ala Ser Val Thr Asp Pro Leu Ile Ala Leu Pro 25 atg gtc ata gtt ttt gtg gca ttg agc atg agt ccc cgc ttg gca agc Met Val Ile Val Phe Val Ala Leu Ser Met Ser Pro Arg Leu Ala Ser 40 WO 01/00805 atc gcc cca Ile Ala Pro atc gca tcc Ile Ala Ser tcc cgc ccc Ser Arg Pro qaa ctc gtt Giu Leu Val caa ggc gtc Gin Gly Vai 115 ctt gcc gcc Leu Ala Ala 130 ggc aac atc Gly Asn Ile 145 qcc gga gca Ala Gly Ala ccc Pro ggc Gly gtc Val1 gtt Val 100 gca Al a g9cg Ala acc Th r aaa Ly s gtt Val1 aaa Lys ttt Phe ccc Pro gtg Val1 gcc Ala acc Thr tca Ser 165 gga Gly gac Asp ttc Phe att Ile cgc Arg ccc Pro 140 aac As n acc Thr aac Asn gcc Ala gtc ValI ccc Pro 125 atg Met g9c9 Ala PCTIIBOO/00926 gtt gcc 192 Val Ala att atc 240 Ile Ile atc atg 288 Ile Met aac ggc 336 Asn Gly gta aac 384 Val Asn ttg atc 432 Leu Ile atc gtc 480 Ile Val 160 <210> 406 <211> 166 <212> PRT <213> Corynebacterium giutamicum <400> 406 Pro Thr Ilie Val Met Ala Met Val 1 5 Leu Asp Leu Ilie Asp Ala Ser Val Met Val Ilie Vai Phe Val Ala Leu Ile Ala Pro Pro Val Ala Val Ala 55 Ile Ala Ser Gly Lys Leu Ala Ser 70 Ser Arq Pro Val Phe Thr Ala Pro Glu Leu Val Val Pro Leu Ala Ilie 100 Gin Gly Val Ala Val Leu Lys Ala Gly 10 Asp Met Val1 Ile Phe 90 Val Gly Ph e Le u Pro Gly Asp Phe Ile Arg Arg Ala Leu Ile Gly Ala Gln 110 Gly Phe Le u Al a Val Ile Ile As n Val1 Gly Pro Ser Ala Ile Met Gly Asn WO 01/00805 115 Leu Ala Ala Ala Ala 130 Gly Asn Ile Thr Thr 145 Ala Gly Ala Lys Ser 165 PCTIBOO/00926 120 125 Ser Gly Leu Trp Ser Leu Pro Met Ala Leu Ile 135 140 Cys Leu Thr Gly Pro Thr Asn Ala Leu Ile Val 150 155 160 His <210> 407 <211> 1280 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..,1257) <223> RXN00571 <400> 407 aca ctt g, Thr Leu V.

1 tca gct gi Ser Ala V.

gtc gcc g( Val Ala G.

gct cta ti Ala Leu SE atg ctc ai Met Leu TI ttc gCg t4 Phe Ala SE Ctt ggc gi Leu Gly V, ttc gCt CE Phe Ala P:

I:

ggt gtc C( Gly Val P: 130 gaa gat gi Giu Asp Vi C ca Pro cat His acc Thr gca Ala gca Ala tcg Ser 99 C Giy 100 gcg Al a 9CC Al a atc Ile cag gtt tac gaa ata gtg Gin Val Tyr Glu Ile Val 5 10 gaa gat cca acc cag atC Glu Asp Pro Thr Gin Ile 25 ctt ggt tcc tac 9CC atg Leu Gly Ser Tyr Ala Met ggt 9CC gtt 99t gac CgC Gly Ala Val Gly Asp Arg 55 atC gtc tgg ttC tCt gtg Ile Val Trp Phe Ser Val 70 att 9Cg ctg ttc ggt ttC Ile Ala Leu Phe Giy Phe 90 atg atc gtt gca aCC 99C Met Ile Vai Ala Thr Gly 105 aat agg CgC aac ttg ttc Asn Arq Arg Asn Leu Phe 120 qgt ggC gtg Ctg gCt tCt Gly Gly Val Leu Ala Ser 135 ggC tgg CgC 998 ctc ttc Gly Trp Arg Gly Leu Phe atC tac ggC gCt gtg Ctg Ile Tyr Gly Ala Val Leu ggt gCg ctc agc CCa gca Gly Ala Leu Ser Pro Ala atc ggc gt9 atg atc ggt Ile Gly Val Met Ile Gly ctt ggt Cgt CgC aaa gtt Leu Gly Arq Arg Lys Val g9c atg gCg Ct9 aCC 9C9 Gly Met Ala Leu Thr Ala 75 ttg cgC ttC Ctc acc 998 Leu Ar9 Phe Leu Thr Giy ggC gCa atC atC gCg gag Gly Ala Ile Ile Ala Giu 110 aac 9Ca. atc gtg taC tCC Asn Ala Ile Val Tyr Ser 125 atC Ctt gca ct9 CtC ttt Ile Leu Ala Leu Leu Phe 140 ctC atC ggt gga tCC CC8 Leu Ile Gly Gly Ser Pro 48 96 144 192 240 288 336 384 432 480 WO 01/00805 145 cta etg ttc Leu Leu Phe cgc tgg ctc Arg Trp Leu gca cgc tat Ala Arg Tyr 195 gaa aca aag Giu Thr Lys 210 atg ggc acc Met Gly Thr 225 ace tac ggc Thr Tyr Gly gca acc tea Ala Thr Ser gca gtg ttc Ala Val Phe 275 gcg aag ace Ala Lys Thr 290 gga gtc ctg Gly Val Leu 305 gca ttc gca Ala Phe Ala ctg acc tcg Leu Thr Ser tgg tgt gca Trp Cys Ala 355 ggt ggc ctg Gly Gly Leu 370 cte Le u acc Th r 180 ggg Gly ggc Gi y att Ilie ctg Leu cat His 260 gg t Gi y gtg Val cca Pro gg C Gly aac As n 340 gg a Giy atc Ile ct 9 Leu 165 tcc Ser ct g Le u ac Th r ctc Le u aac Asn 245 ga t Asp ggc Gly atc Ilie aac As n qtC Val1 325 ttc: Phe ttC Phe atc Ile 150 cca Pro cgc Arg ccg Pro gg a Gly atc Ile 230 acc Thr tcc Se r ctc Le u acc Th r atc Ile 310 qge Gi y tte Phe gge Gly gg C Gi y ctt Le u gg e Gly acg Th r ttc Phe 215 ggc Gly tgg T rp ctg Le u ate Ilie tee Ser 295 tee Ser gte Val1 gga Gly eg a Arg gca Ala 375 gca tac ttc Ala Tyr Phe 170 cgt get gcg Arg Ala Ala 185 gag gaa ttt Giu Giu Phe 200 gct gga att Ala Gly Ile gca atg aqc Ala Met Ser ttg cca aag Leu Pro Lys 250 tac tcc ctg Tyr Ser Leu 265 gca tcc tg Ala Ser Trp 280 acc ttc get Thr Phe Ala tcc tgg cca Ser Trp Pro ctg ggc acc Leu Gly Thr 330 acc gaa tge Thr Glu Cys 345 etc ggc gga Leu Gly Gly 360 gga ttc gga Gly Phe Gly atc ggc gcg Ile Gly Ala 155 ttc Phe gac Asp gtc Val1 tt C Phe ttc Ph e 235 atc Ilie ctg Leu ttc Phe ct C Leu atg Met 315 ca 9 Gin cgc Arg atc Ile cea Pro gte Val1 395 ctc Leu gcc Ala gtc Val tcc Se r 220 ate Ile atg Met ttc Phe get Al a gcc Ala 300 atg Met gt t ValI gca Ala gte Val a gc Se r 380 t gc Cys cca Pro aaa Lys gaa Giu 205 tcc Ser ggg Gly gaa Giu ctc Leu gac Asp 285 geg Ala t ac Tyr ctc Le u gcg Ala 9ga Gly 365 tee Ser acc Thr gag Glu gcc Ala 190 aag Lys aag Lys ctg Leu t cc Ser aac As n 270 cgc Arg ate Ile ace Thr acc Th r gga Gly 350 eca Pro gca Ala ttg Leu PCT/IBOO/00926 160 tee ceg 528 Ser Pro 175 etc tge 576 Leu Cys eag eag 624 Gin Gin tac etc 672 Tyr Leu ctt teg 720 Leu Ser 240 aac ggc 768 Asn Gly 255 gge gge 816 Gly Gly ate ggc 864 Ile Gly tge etc 912 Cys Leu gca ate 960 Ala Ile 320 tac ggc 1008 Tyr Gly 335 gtt gea 1056 Val Ala gca ate 1104 Ala Ilie ttCcetc 1152 Phe Leu etg ate 1200 Leu Ile 400 ate tte gca gca get gee gca Ile Phe Ala Ala Ala Ala Ala WO 01/00805 PCTIiBOO/00926 ccg cgc tcc cca gca gaa gta gag gtc aag gtc gcg cag gaa cca ctt 1248 Pro Arg Ser Pro Ala Glu Val Glu Val Lys Val Ala Gin Glu Pro Leu 405 410 415 gca cgt gtc taaccccaat taattcgaaa caa 1280 Ala Arg Val <210> 408 <211> 419, <212> PRT <213> Corynebacterium glutamicum <400> 408 Thr Leu Val Pro Gin Val Tyr Giu Ile Val Ile Tyr Gly Ala Val Leu 1 5 10 Ser Ala Val His Glu Asp Pro Thr Gin Ile Gly Ala Leu Ser Pro Ala 25 Val Ala Gly Thr Leu Gly Ser Tyr Ala Met Ile Gly Val Met Ile Gly 40 Ala Leu Ser Ala Gly Ala Val Gly Asp Arg Leu Gly Arg Arg Lys Val 55 Met Leu Thr Ala Ile Val Trp Phe Ser Val Gly Met Ala Leu Thr Ala 70 75 Phe Ala Ser Ser Ile Ala Leu Phe Gly Phe Leu Arg Phe Leu Thr Gly 90 Leu Gly Val Gly Met Ilie Val Ala Thr Gly Gly Ala Ile Ile Ala Giu 100 105 110 Phe Ala Pro Ala Asn Arg Arg Asn Leu Phe Asn Ala Ile Val Tyr Ser 115 120 125 Gly Val Pro Ala Gly Gly Val Leu Ala Ser Ile Leu Ala Leu Leu Phe 130 135 140 Giu Asp Val Ile Gly Trp Arg Gly Leu Phe Leu Ile Gly Gly Ser Pro 145 150 155 160 Leu Leu Phe Leu Leu Pro Leu Ala Tyr Phe Phe Leu Pro Glu Ser Pro 165 170 175 Arg Trp Leu Thr Ser Arg Gly Arg Ala Ala Asp Ala Lys Ala Leu Cys 180 185 190 Ala Arg Tyr Gly Leu Pro Thr Glu Glu Phe Val Val Giu Lys Gin Gin 195 200 205 Glu Thr Lys Gly Thr Gly Phe Ala Gly Ile Phe Ser Ser Lys Tyr Leu 210 215 220 Met Gly Thr Ile Leu Ilie Gly Ala Met Ser Phe Ile Gly Leu Leu Ser 225 .230 235 240 WO 01/00805 Thr Tyr Gly Ala Thr Ser Ala Val Phe 275 Ala Lys Thr 290 Gly Val Leu 305 Ala Phe Ala Leu Thr Ser Trp Cys Ala 355 Gly Gly Leu 370 Ile Phe Ala 385 Pro Arg Ser Ala Arg Val Le u H is 260 Gly Vai Pro Gly Asn 340 Gly Ile Ala Pro Asn Thr 245 Asp Ser Gly Leu Ile Thr Asn Ile 310 Val Gly 325 Phe Phe Phe Gly Ile Gly Ala Ala 390 Ala Giu 405 Trp Leu Ile Ser 295 Ser Val1 Gly Arq Al a 375 Ala Val Le u Tyr Ala 280 Thr Ser Leu Th r Leui 360 Gi y Ile Glu Pro Se r 265 Se r Phe Trp Gly Glu 345 Gly Ph e Gly Val Lys 250 Leu T rp Ala Pro Th r 330 Cys Gly Gly Al a Lys 410 Ile Leu Phe Leu Met 315 Gin Arg Ile Pro Val1 395 Val1 Met Phe Ala Ala 300 Met Val1 Al a Val1 Ser 380 C ys Al a Giu Leu Asp 285 Ala Tyr Le u Ala Gly 365 Se r Th r Gln PCTLBOO/00926 Asn Gly 255 Gly Gly Ile Gly Cys Leu Ala Ile 320 Tyr Gly 335 Val Ala Ala Ile Phe Leu Leu Ile 400 Pro Leu 415 <210> 409 <211> 1208 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(1185) <223> FRXA00571.

<400> 409 ca.g atc ggt gcg ctc agc cca gca gtc Gin Ile Gly Ala Leu Ser Pro Ala Vai 1 5 gcc atg atc ggc gtg atq atc ggt gct Ala Met Ile Gly Val Met Ile Gly Ala 25 gac cgc ctt ggt cgt cgc aaa gtt atg Asp Arg Leu Gly Arg Arg Lys Val Met 40 tct qtg ggc atg gcg ctg acc gcg ttc gcc ggc acc ctt ggt tcc tac Ala Gly Thr Leu Gly Ser Tyr 10 cta tct gca ggt gcc gtt ggt Leu Ser Ala Gly Ala Val Gly ctc acc gca atc gtc tgg ttc Leu Thr Ala Ile Val Trp Phe gcg tcc tcg att gcg ctg ttc WO 01/00805 PCT/IBOO/00926 Ser Val Gly Met Ala Leu Thr Ala Phe Ala Ser Ser Ile Ala Leu Phe ggt Gly acc Thr ttg Le u gct Ala ct C Leu tac T yr 145 gct Ala gaa Giu gga Gi y atg Met cca Pro 225 t CC Se r t cc Se r ttc Phe tgg Trp ttC ttg Phe Leu 99c ggc Gly Gly ttc aac Phe Asn tct atc Ser Ile 115 ttc ctc Phe Leu 130 ttc ttc Phe Phe gcg gac Ala Asp ttt gtc Phe Val att ttc Ilie Phe 195 agc ttc Ser Phe 210 aag atc Lys Ile ctg ctg Leu Leu tgg ttc Trp Phe gct ctc Ala Leu 275 cca atg Pro Met cgc Arg gca Al a gc a Al a 100 ct t Leu atc Ilie ctc Leu gcc Ala gtC Val1 180 t CC Ser atc Ile atg Met ttc Phe qc t Al a 260 gcc Ala a tg Met ttc Phe atc Ile atc Ile gca Ala ggt Gi y cca Pro aaa Lys5 165 gaa Glu tcC Se r ggg Gly gaa Glu ctc Leu 245 gac Asp gc9 Ala tac T yr ctc Leu atc Ile gtg Val ctg Leu gga Gly gag Giu 150 gcc Ala aag Lys a a g Lys ctg Le u tcc Ser 230 aac Asn cgc Arg atc Ile acc Thr 55 acc Thr g cg Ala tac Tyr ct C Le u tcc Ser 135 tcc Se r ct C Le u cag Gin tac T yr ctt Leu 215 aac Asn 9gc Gly atc Ilie tgc Cys g ca Ala ggc Gly gct Ala gt C ValI ga t Asp.

ctg Le u tgg T rp cgc Arg 170 aca Thr ggc Gly tac T yr acc Thr gtg Val 250 aag Lys gtc Val1 ttc Phe ggc Gly gcg Al a gcc Ala atc Ile ctc Leu 140 acc Thr gqq Gly ggc Gly att Ile ctg Leu 220 cat His ggt Gly gt g Val1 cca Pro gg C Gly a tg Met aat As n ggt Gly ggc Gly 125 ct 9 Leu tcc Se r Ctg Le u acc Th r ctc Le u 205 aac As n ga t Asp 9 gC Gl y atc Ile aac Asn 285 gtc ValI atc Ile -a ggj Arg 99C Gi y 110 t 99 Trp CCa Pro cCC Arg CCg Pro gga Gly 190 atc Ile acc Th r t CC Se r ctc Leu acc Thr 270 atc Ile 9gC Gly WO 01/00805 290 295 ggc acc cag gtt ctc acc tac ggc ctg Gly Thr Gin Val Leu Thr Tyr Gly Leu 305 310 gaa tqc cgc gca gcg gga gtt gca tgg Glu Cys Arg Ala Ala Giy Val Ala Trp 325 ggc gga atc gtc gga cca gca atc ggt Gly Gly Ilie Val Gly Pro Ala Ile Gly 340 345 ttc gga cca aqc tcc gca ttc ctc atc Phe Giy Pro Ser Ser Ala Phe Leu Ile 355 360 ggc gcg gtc tgc acc ttg ctg atc ccg Gly Ala Val Cys Thr Leu Leu Ile Pro 370 375 gtc aag gtc gcg cag gaa cca ctt gca Val Lys Val Ala Gin Glu Pro Leu Ala 385 390 caa <210> 410 <211> 395 <212> PRT <213> Corynebacterium giotarnicom <400> 410 Gin Ilie Giy Ala Leu Ser Pro Ala Vai 1 5 Ala Met Ilie Gly Vai Met Ilie Giy Aia 25 Asp Arg Leo Giy Arg Arg Lys Vai Met 40 Ser Val Giy Met Ala Leu Thr Ala Phe Gly Phe Leu Arg Phe Leu Thr Gly Leu 70 Thr Gly Gly Ala Ilie Ilie Ala Giu Phe Leu Phe Asn Ala Ile Val Tyr Ser Gly 100 105 Ala Ser Ilie Leu Ala Leo Leu Phe Giu 115 120 Leu Phe Leo Ile Giy Gly Ser Pro Leo 130 135 PCT/IBOO/00926 300 aac ttc ttc gga acc 960 Asn Phe Phe Gly Thr 320 gga ttc ggc cga ctc 1008 Gly Phe Giy Arg Leu 335 atc atc ggc gca gga 1056 Ile Ile Gly Aia Giy 350 gca gct gcc gca atc 1104 Aia Ala Aia Aia Ile 365 cca gca gaa gta gag 1152 Pro Ala Glu Val Glu 380 taaccccaat taattcgaaa 1205 1208 Ala 10 Leu Leo Aia Gly Al a 90 ValI Asp Leu Gb y Ser Th r Se r Val1 75 Pro Pro Val Phe Leo Giy Ile Ile Met Asn Gly Gly 125 Leo Giy Ala Vali Ala Ile Arg Gi y 110 Trp Pro Se r Val1 Trp Leo Val Arg Val1 Arg Leo Tyr Gly Phe Phe Al a Asn Leo Gly Ala WO 01/00805 Tyr Phe P) 145 Ala Ala A! Glu Phe Vi Gly Ile P1 Met Ser P) 210 Pro Lys I.' 225 Ser Leu L( Ser Trp P1 Phe Ala L( Trp Pro M( 290 Gly Thr G1 305 Glu Cys PA Gly Gly Ii' Phe Gly Pi Gly Ala Vi 370 Val Lys Vi 385 <210> 411 <211> 689 <212> DNA Glu Ser 150 Ala Leu Lys Gin Lys Tyr Leu Leu 215 Ser Asn 230 Asn Gly Arg Ilie Ile Cys Thr Ala 295 Thr Tyr 310 Gly Val Pro Ala Ala Phe Leu Leu 375 Arg Ala Glu 185 Met Thr Ala Ala Al a 265 Gi y Ala Le u Trp Gly 345 Ile Pro Trp Arg 170 Thr Gi y Tyr Thr ValI 250 Ly s Val1 Phe Thr Cys 330 Gly Phe Arg Le u 155 T yr Lys Thr Gly Se r 235 Phe Thr Le u Ala Se r 315 Ala Leu Ala Se r Arg Pro Gly 190 Ile Thr Se r Leu Th r 270 Ile Gly Phe Gly Gly 350 Ala Glu PCTIBOOIOO926 Gly Arg 160 Thr Glu 175 Phe Ala Gly Ala Trp Leu Leu Tyr 240 Ile Ala 255 Ser Thr Ser Ser Val Leu Gly Thr 320 Arg Leu 335 Ala Gly Ala Ile Val Glu Ala Gin Giu Pro Leu Ala Arg Val <213> <220> <221> <222> <223> <400> Corynebacterium glutarnicum C DS (666) RXA0 0962 411 WO 01/00805 gat aag gcc Asp Lys Ala 1 cca gca ggt Pro Ala Gly att tgg ttc Ilie Trp Phe ctg ggc aca Leu Gly Thr ggc cat gca Gly His Ala gga tcc gtg Gly Ser Val tcc ggt gtc Ser Giy Val ctc act tac Leu Thr Tyr 115 ggc gtg ggc Gly Val Gly 130 aac ttc tac Asn Phe Tyr 145 ggt gta ggt Gly Val Gly ctg ctg gca Leu Leu Ala acc gct gcg Thr Ala Ala 195 cag aaa acc gcg Ala ga t Asp qcc Ala cc t Pro ttc Phe 70 gcg Ala gc a Al a gt c Val ggC Gly aac As n 150 ggt Gly ttg Leu t ct Ser gtc ga t Asp ct 9 Le u aca Th r cgc Arg acc Thr tgg Trp gg t Gly a cc Thr act Thr 135 ctg Le u g ct Ala ggc Gi y g cg Ala a cc ggc Gly cg c Arg g cg Ala gg t Gly ggt Gly gg g Gly ct g Leu ctc Le u 125 g ca Ala ggC Gly ctc Leu atc Ile t tg Leu 205 cac His tgg Trp tgg T rp tat Tyr gca Al a cca Pro ctc Leu 110 att Ile gct Ala t9g Trp gct Ala atg Met 190 ctg Le u PCTIBOO/00926 gat gag 48 Asp Giu atc tcc 96 Ile Ser tac gga 144 Tyr Gly gag ttc 192 Glu Phe gtg atc 240 Val Ile atc cgt 288 Ile Arg ctg ctg 336 Leu Leu ttg gct 384 Leu Ala gtc gcc 432 Val Ala gcg cta 480 Ala Leu 160 ggc ctg 528 Gly Leu 175 ttc ggc 576 Phe Gly cgc ctg 624 Arg Leu 666 cac aaa gtc gaa atc caa ggc Gin Lys Thr Tyr Ser Val Thr His Lys Val Giu Ile Gln Gly taaccccctt tttcaactca cag <210> 412 <211) 222 WOi 01/00805 <212> PRT <213> Corynebacterium qiutamicum PCT[IiBOO/00926 <400> 412 Asp Lys Ala Ilie Lys Ala Asp His Asp Ile Arg Glu Gly His Asp Giu 1 Pro Ile Leu Gly Gly Ser Le u Gly Asn 145 Gly Leu Thr Gin Ph e Al a Trp Leu Val1 Ile 100 Pro Thr Pro Arg Trp 180 Leu T yr Asp Ala Pro Phe 70 Ala Ala Val Gly Asn 150 Gly Le u Ser Val1 Le u Thr Arg Th r Trp Gly Thr Thr 135 Leu Ala Gly Ala Thr 215 Le u Ph e Le u Leu Al a Ile Al a 120 Gin Arg Val1 Val Le u 200 His 10 Val Asp 25 Vai Thr Met Glu Ala Leu Gly Asp Ala Gly 105 Val Tyr Ile Leu Gly Thr Val Ala 170 Asn Ser 185 Ala Leu Lys Val Trp Trp T yr Ala Pro Leu 110 Ile Ala Trp Al a Met 190 Leu Gly Ile Tyr Glu Val Ile Le u Le u Val Al a Gly 175 Phe Arg Ser Giy Phe Ile Arg Leu Al a Ala Le u 160 Le u Gly Leu <210> 413 <211> 484 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (484) <223> RXA02811 <400> 413 cataatcact ctttccagat agcggaatcc ctttgattgt aacttggctc acctcgatta tgtqtttatg acatcacacg caccaqaatc gggaggactt gtg aca gag tca act 115 WO 01/00805 ctc Leu atc Ilie ttt Phe caa Gin acc Th r att Ilie t cc Se r gga Gi y ttg Leu ttt Phe t cg Ser atc Ile ggt Gly gtg Val1 gca Ala Giy gtc Vali gCC Ala ggc Gi y cga Arg ctg Leu t cg Se r atc Ilie att Ilie ctg Le u tcc Ser ct g Le u att Ilie 105 gtg Vali aat Asn a gc Se r ttc Phe gct Aila acc Th r tca Se r ctc Leu agc Se r ggC Giy ga c Asp aa 9 Ly s qcg Ala 75 gac Asp t ct Ser t cc Ser cg a Arg 9gc Gly gag Giu 60 t tc Phe cgc Arg gic Val1 caa Gin cg C Arg tac T yr 45 tgg Trp ttt Phe gtg Val1 ttc Phe aca Thr t tg Leu 30 gac Asp aac As n ggc Gi y Gly acc Thr 110 att Ile 15 gct Aia ctC Leu tta Le u atg Met cga Arg 95 atg Met g aa Glu gcg Aila att Ile agc Se r gcg Aia 80 aaa Lys ctg Le u Val 1 aat Asn gtg Val1 gt g Val tct Se r atc Ile g ca Ala tqt Cys Thr aaa Lys ctc Leu tac Tyr gca Aila Gly gcg Ala qca Aila Giu Giy att Ile ggc Giy a cg Thr gct Al a gtg Val1 ttt Phe 115 ci Li 9'

G

a

T

C

L

9

V

a 9

A

PCT[IBOO/00926 ~rThr tc acC 163 eu Thr 9c tgg 211 ly Trp cc gtc 259 hr Val tg ggc 307 eu Gly tg ttc 355 ai Phe tt gga 403 le Gly 00 Ct. cca 451 lia Pro aac cca tgg Asn Pro Trp ttc ggc qct ttc cgt ttC atc Phe Gly Ala Phe Arg Phe Ile 125 <210> 414 <211> 128 <212> PRT <213> Corynebacterium giutamicun (400> 414 Val Thr Giu Ser Thr Leu Gly Ala Ser 1 5 Asn Lys Gly Leu Thr Ile Leu Gly Ile 25 Val Leu Ile Gly Trp Phe Phe Vai Ile 40 Val Tyr Gly Thr Val Gin Ser Ala Leu 55 Ser Ala Thr Leu Gly Thr Ilie Gly Ser 70 Ilie Gly Ala Val Phe Ilie Gly Arg Leu Ala Ala Val Ile Gly Ser Val Leu Ile Asn 10 Se r Phe Ala Thr Se r 90 Le u Se r Gly Asp Lys Ala 75 Asp Ser Ser Arg Gly Giu Phe Arg Val Gin A rg Tyr Trp Phe Val Phe Th r Le u Asp As n Gi y Gly Thr Ile Ala Leu Le u Met Arg Met Glu Ala Ile Se r Ala Lys Leu WO 01/00805 PCTJIBOO/00926 100 105 110 Cys Ala Phe Ala Pro Asn Pro Trp Val Phe GlY Ala Phe Arg Phe Ile 115 120 125 <210> 415 <211> 1197 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1)..(1197) <223> RXA02115 <400> 415 acc cgc gca acc aaa agi gtc gga aca gtt ctc gca ctc ctg tgg ttc 48 Thr Arq Ala Thr Lys Ser Val Gly Thr Val Leu Ala Leu Leu Trp Phe 1 5 10 *gca att gtc ctc gac ggc ttt gac cta gtc gtc ctg ggt gca aca atc 96 Ala Ilie Val Leu Asp Gly Phe Asp Leu Val Val Leu Gly Ala Thr Ile 25 ccg tcc atg ctg gag gat ccc gcg tgg gat ctc act qct gga cag gcc 144 Pro Ser Met Leu Glu Asp Pro Ala Trp Asp Leu Thr Ala Gly Gin Ala 40 aca cag att tcc acc atc qqc ctc gtc ggc atg acc att ggc gca ctg 192 Thr Gin Ile Ser Thr Ile Gly Leu Val Gly Met Thr Ile Gly Ala Leu 55 acc atc ggt ttc tta acg gac cgt ctg ggt cga cgc cgt gtc atg ctg 240 Thr Ile Gly Phe Leu Thr Asp Arg Leu Gly Arg Arg Arg Val Met Leu 70 75 ttc tct gtg gca gta ttt tct gta ttc act ctc ctg ctg gca ttc acc 288 Phe Ser Val Ala Val Phe Ser Val Phe Thr Leu Leu Leu Ala Phe Thr 90 acc aac gtc cag ctc ttc agc ctg tgg cgt ttc ctc gca ggt gtt ggc 336 Thr Asn Val Gin Leu Phe Ser Leu Trp Arg Phe Leu Ala Gly Val Gly 100 105 110 ctt ggt gga gca ctc ccc acc gca att gcc atg gtg acc gag ttc cgc 384 Leu Gly Gly Ala Leu Pro Thr Ala Ile Ala Met Val Thr Glu Phe Arg 115 120 125 ccc ggc acc aaa gcg ggc tct gca tcc acc acc ttg atg acc ggc tac 432 Pro Gly Thr Lys Ala Gly Ser Ala Ser Thr Thr Leu Met Thr Gly Tyr 130 135 140 cac gtc ggc gca gta gca acc gcc ttc ctt ggc ctc ttc ctt atc gac 480 His Val Gly Ala Val Ala Thr Ala Phe Leu Gly Leu Phe Leu Ile Asp 145 150 155 160 ggc ttt ggt tgg cac tcc atg ttc atc gca ggt gcc gta cca gga ctq 528 WO 01/00805 Gly Phe Gly atc ctg cta Ile Leu Leu ctc aaa atc Leu Lys Ile 195 tat gga ctt Tyr Gly Leu 210 ctc ggc gag Leu Gly Giu 225 cgc aac acc Arg Asn Thr gtc tac ggc Val Tyr Gly tac qac atq Tyr Asp Met 275 gqa gtg atc Ala Vai Ile 290 cct cgc aaa Pro Arg Lys 305 gcg ttg ctt Ala Leu Leu ctg ctc acc Leu Leu Thr ttc gtt ggt Phe Val Gly 355 ttc tcc gca Phe Ser Ala 370 ggt ggt ctg Gly Gly Leu 385 <210> 416 Trp c ca Pro 180 tcc Se r t CC Ser tcc Ser ctq Le u ctg Leu 260 ggt Gly 9gc Gly aca Thr gct Al a ggC Gly 340 gag Glu gg a Gly ctt Le u His 165 Ctg Le u gg c Gly ct g Le u t CC Se r gcg Al a 245 aac As n aac Asn ctt Le u gca Al a gt C Val1 325 atc Ile aat Asn att Ile gtc Val Ser ctg Leu aag Lys ga t Asp t ca Ser 230 att Ile aca Th r t cg Se r tat Tyr Ctc Le u 310 cg g Arg ttt Phe cac His gg t Gly agt Ser 390 Met tac Tyr ttg Leu ga t Asp 215 ctt Le u t gg Trp t gg Trp ctt Leu att Ile 295 gta Val1 atg Met gtg Val1 ct Pro cgc Arg 375 gcc Ala Phe ttc Ph e ga t Asp 200 ga t Asp tcc Ser gg C Gly ctg Le u gga Gly 280 gca Al a tgg Trp cca Pro ttc Phe gcc Al a 360 ctc Le u aac Asn Ile ttc Phe 185 gag Glu ctt Leu tCC Ser acc Th r cca Pro 265 ttc Ph e ggg Gly ttc Ph e ct g Le u agc Se r 345 aag Lys ggc Gly ctt Le u Al a 170 ctt Leu gcg Ala ga t Asp ct g Leu t ca Ser 250 caa Gin ctc Leu cga Arg gtg Val1 atc Ile 330 tcc Ser atg Met gcc Ala gc t Al a Giy cca Pro cag Gin cgc Arg ttC Phe 235 ttc Phe atc Ile atg Met att Ile ttc Phe 315 gg t Gly cag Gin cgc Arg atc Ile tac Tyr 395 Al a gaa Glu gca Al a gaa Gi u 220 aag Lys atg Met atg Met gtg Val1 gcc Ala 300 tct Ser ctg Leu gta Val1 gca Ala tCt Se r 380 cca Pro Val1 t CC Se r gt t ValI 205 ca c His

CCC

Pro gga Gi y cg C Arg ctc Leu 285 gat Asp g ca Ala tat Tyr ct C Le u acc Thr 365 9gc Gly t gg Trp Pro ccg Pro 190 gca Ala gaa Giu t cg Ser CtC Le u caa Gin 270 aac Asn aag Lys tt t Phe ggc Gl y atc Ile 350 gcc Al a ccg Pro ggc Gly PCTIBOO/00926 Gly Leu 175 cag tac 576 Gin Tyr gca tct 624 Ala Ser gaa gaa 672 Giu Glu ttc cgc 720 Phe Arg 240 ctc ctg 768 Leu Leu 255 gca gac 816 Ala Asp atc ggc 864 Ile Gly aac tcc 912 Asn Ser tcc ctc 960 Ser Leu 320 atc gtg 1008 Ile Val 335 tac gcc 1056 Tyr Ala atg gga 1104 Met Gly ttg ctt 1152 Leu Leu ttc 1197 Ph e WO 01/00805 <211> 399 <212> PRT <213> Corynebacterium glutamicum PCT/IBOO/00926 <400> 416 Th r 1 Ala Pro Th r Th r Ph e Th r Le u Pro His 145 Gly Ile Le u Tyr Leu 225 Arg Val1 Tyr Al a Arq Ala Thr Lys Ser Val Gly Thr Ile Ser Gin Ile Ser Asn Gly Gly 130 Val Phe Le u Lys Gly 210 Gly As n Ty r Asp Val1 290 Val1 Met Ile Gly ValI ValI Gly 115 Th r Gly Gi y Leu Ile 195 Leu Giu Th r Gi y Met 275 Ile Le u Leu Ser Phe Al a Gin 100 Al a Lys Ala Trp Pro 180 Se r Se r Se r Le u Le u 260 Gly Gly Asp Al a Leu Arg Val Leu Ala 120 Ala Al a Phe Phe Asp 200 Asp Se r Gly Le u Gly 280 Al a Le u Trp Val Leu Ph e Trp 105 Ile Se r Phe Ile Phe 185 Glu Le u Se r Th r Pro 265 Ph e Gly Val Val1 Asp Gly Gly Thr Arg Ala Th r Leu Ala 170 Le u Ala Asp Leu Ser 250 Gin Leu Arg WO 01/00805 Pro Arg Lys Thr Ala Leu Val Trp Phe Val Phe 305 310 315 Ala Leu Leu Ala Val Arg Met Pro Leu Ile Gly 325 330 Leu Leu Thr Gly Ile Phe Val Phe Ser Ser Gin 340 345 Phe Val Gly Glu Asn His Pro Ala Lys Met Arg 355 360 Phe Ser Ala Gly Ile Gly Arg Leu Gly Ala Ile 370 375 Gly Gly Leu Leu Val Ser Ala Asn Leu Ala Tyr 385 390 395 <210> 417 <211> 1288 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1288) <223> RXN00590 <400> 417 tctaaactca ctctcaactc accaagattg ttcaacaatc ccccaatcat tttgaaagcc cccacgaaag gagcgcqaca PCTIBOOIOO926 Al a Tyr Leu Thr 365 Gly Trp, aat As n cct Pro gt a Val1 g ca Ala acc Thr ctc Le u cta Le u

C

Ala agc Ser cca Pro gct Ala cag Gin agc Ser t cc Ser gg t Gl y acc Th r gcg Ala 80 tgq Trp tgcgattggt gtgcaatcta atg gcc gac aac aaa Met Ala Asp Asn Lys 1 act gga acc cct ggg Thr Gly Thr Pro Gly ctc aag caa gct gca Leu Lys Gin Ala Ala gcc atc ttc ctc atg Ala Ile Phe Leu Met caa acc gct gtc ttc Gin Thr Ala Val Phe atc ctg gtg tcg atc Ile Leu Val Ser Ile cgc atc atc ggc gtc Arg Ile Ile Gly Val 100 WO 01/00805 tct gaa atg cgC gcc caa gaa ctc ggc aac acg PCTIBOOIOO926 Se r gg t Gly atc Ile tgg Trp 150 atc Ile gt c Val1 t cc Ser a cc Thr gga Gly 230 acc Th r atc Ile 9t Gly gcg Ala ggc Gly 310 tac Tyr Ctg Glu tgg Trp ggc Gly 135 ga c Asp ttc Phe ctc Leu caa Gin atc Ile 215 tac Tyr ggc Gly ctg Leu qtt Val1 gaa Glu 295 9C Ala ac Thr caa Met gtg Val1 120 aac Asn gtc Val1 ttg Leu 99c Gly CCt Pro 200 gac Asp atc Ile ccc Pro atc Ile gtc ValI 280 gca Al a gtg Val1 tCt Se r aac Arg 105 ctg Leu atc Ile aaa Lys ttC Phe gtc Val 185 cca Pro tyg T rp act Thr aac As n act Thr 265 g ca Ala ttc Phe ct q Le u g Ca Al a tyg Ala qcc Ala gcc Al a gtt Val1 aag Lys 170 gtg Val1 gtt Val1 ctt Le u tac Tyr aa c As n 250 9qc Gly ggt Gi y cag Gin t gg Trp acc Th r 330 gtg Gin gta Val 9gt Gi y gg t Gly 155 c ga Arg at 9 Met ggc Gly 9tc Vak 1 gct Ala 235 gtc Val1 ctc Le u ggc Gly cac His qca Ala 315 ttc Phe acc Giu Ct g Leu gg t Gly 140 ggC Gi y Ctt Le u atc Ile t Cg Ser atc Ile 220 9gc Gi y aaa Lys atg Met gtc Val1 gct Ala 300 gcg Ala Ct 9 Le u atc Leu gt c Val1 125 ggc Gly gtg Val1 99 t Gly Ctg Le u gCg Ala 205 acc Th r gca Ala 9ct Al a cgC Arg acc Thr 285 gca Al a tcc Ser gtg Val1 atc Gi y 110 t gt Cys Ctt Le u atc Ile 9 ct Ala ctc Leu 190 Ctg Leu aca Thr caC His gtt Val1 gtg Val1 270 cta Le u ggC Gly att Ile gaa Gi u ttc As n att Ile ggg GI y acc Th r gct Ala 175 acc Th r aag Lys Ctt Leu Cg c Arg t CC Ser 255 gtg Val tcc Ser gat Asp tcc Ser aac As n 335 atc Thr 9gc Gly ctt Leu gCg Ala 160 Ctg Le u gt C Val1 aat Asn gt q Val1 atg Met 240 aat Asn ctc Le u acc Thr atc Ile tca Ser 320 aag Lys ctg gtt Val1 ggC Gi y aac As n 145 gCC Ala gac Asp taC Tyr g ca Ala ggt Gly 225 Ct 9 Le u tcc Ser ttC Phe a Cg Thr 99 a Gi y 305 gtc Val cca Pro att atC CCa 9gt Ile Pro Gly 115 gta gta ttC Val Val Phe 130 9Cg Ct9 Ctt Ala Leu Leu atC gcg att Ile Ala Ile aaa ttC ctC Lys Phe Leu 180 gtg gCt ttC Val Ala Phe 195 gta Ctt CCt Val Leu Pro 210 qqa acc gtC Gly Thr Val gac tcc gqa Asp Ser Gly tCt atC aCC Ser Ile Thr 260 CtC 9C9 gtt Leu Ala Val 275 ggC aac cca Gly Asn Pro 290 cta CgC atC Leu Arg Ile atc ggC 9CC Ile Gly Ala gag aag aag Giu Lys Lys 340 tct tgC tC ttt Phe aac Asn 9gc Gly 9C9 Ala 165 gtg Val1 gtc Val1 ga C Asp gqt G ly.

cg a Arg 245 9gc Gly CtC Leu gCC Ala ttc Phe agc Se r 325 cgt A rg gtg 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 WO 01/00805 PCTIBOO/00926 Leu Gin Asn Trp Val Thr Ile Ile Phe Ile Leu Ile Ser Cys Ser Val 345 350 355 ttc atc atg ctc ggc acg gca cca gca atc ctc ttg gtc ttc gcc gga 1219 Phe Ile Met Leu Gly Thr Ala Pro Ala Ile Leu Leu Val Phe Ala Gly 360 365 370 gca ttc aac ggt ttg gtc ctc ccc gta ggc ttt acc ctg atg atc tac 1267 Ala Phe Asn Gly Leu Val Leu Pro Val Gly Phe Thr Leu Met Ile Tyr 375 380 385 gta gcg atc ttc cgc caa aaa 1288 Val Ala Ile Phe Arg Gin Lys 390 395 <210> 418 <211> 396 <212> PRT <213> Corynebacteriun giutarnicum <400> 418 Met Ala Asp Asn Lys Asn Ala Asp Asp 1 Th r Leu Ala Gin Ile Arg Val1 Gi y As n 145 Ala Asp Tyr Thr Gin Phe Ala Val1 Ile Pro 115 Val1 Leu Ala Phe Ala 195 Pro Ala Leu Val Se r Gly 100 Gly Phe Le u Ile Leu 180 Phe 5 Gly Ala Met Phe Ile Vali Phe Asn Gly Ala 165 Val1 Val1 Ser Ala Gly Ile Gly Ala Ala Ala Ala Val1 Lys 170 Val1 ValI Gin Lys Arq Gly Al a 75 ValI Gin Val1 Gly Gly 155 Arq Met Gly Se r Se r Gly Thr Ala Trp Th r Gly Le u Al a 160 Le u Val1 Asn WO 01/00805 Ala Val Leu 210 Gly Gly Thr 225 Leu Asp Ser Ser Ser Ile Phe Leu Ala 275 Thr Gly Asn 290 Gly Leu Arg 305 Val Ile Gly Pro Glu Lys Ile Ser Cys 355 Leu Val Phe 370 Thr Leu Met 385 <210> 419 <211> 487 <212> DNA Pro Val1 Gly Th r 260 Val1 Pro Ile Al a Ly s 340 Se r Ala Ile Asp Gi y Arg 245 Gi y Le u Al a Phe Ser 325 Arg Val Gly T yr Thr Gly 230 Thr Ile Gly Ala Gly 310 Tyr Le u Phe Al a Val1 390 Ile 215 Tyr Gly Le u Val Glu 295 Ala Th r Gin Ile Phe 375 Al a Asp Ile Pro Ile Val1 280 Ala ValI Ser As n Met 360 As n Ile Trp Th r As n Th r 265 Al a Phe Leu Al a T rp 345 Le u Gly Phe Le u Tyr As n 250 Gly Gly Gin Trp Thr 330 Val Gi y Le u Arg Val1 Al a 235 Val1 Le u Gly His Al a 315 Phe Thr Thr Val1 Gin 395 Ile 220 Gly Lys Met Val1 Al a 300 Ala Le u Ile Ala Le u 380 Lys Th r Ala Al a Arg Thr 285 Ala Ser Val1 Ile Pro 365 Pro Th r His ValI Val1 270 Leu Giy Ile Glu Phe 350 Ala Val1 PCTIiBOO/00926 Leu Val Arg Met 240 Ser Asn 255 Val1 Leu Ser Thr Asp Ile Ser Ser 320 Asn Lys 335 Ile Leu Ile Leu Gly Phe <213> Corynebacterium giutamicun <220> <221> CDS <222> (487) <223> FRXA00590 <400> 419 tacgctggcg cacaccgcat gctggactcc ggacgaaccg gccccaacac gtcaaagctg tttccaattc tctatcaccg gcatcctgat cactggcctc atg cgc gtg gtg ctc Met Arg Val Val Leu 1 ttc ctc gcg gtt ctc ggt gtt gtc gca ggt ggc gtc acc cta tcc acc Phe Leu Ala Val Leu Gly Val Val Ala Gly Gly Val Thr Leu Ser Thr 15 acg ggc aac cca gcc gcg gaa gca ttc caq cac gct gca ggc gat atc Thr Gly Asn Pro Ala Ala Glu Ala Phe Gin His Ala Ala Gly Asp Ile 30 WO 01/00805 gga Cta cgc Gly Leu Arg gtc atc ggc Val Ile Gly cca gag aag Pro Glu Lys att tct tgc Ile Ser Cys ttg gtc ttc Leu Val Phe acc ctg atg Thr Leu Met 120 atc Ile qCC Al a aag Lys tc Se r gc c Ala 105 atc Ile ttc Phe agc Se r cg t Arg gtg Val gga Gly tac Tyr ggc: Gi y tac Tyr Ct g Le u 75 ttc Phe g ca Ala gta Val1 gcc Al a 8CC Th r 60 caa Gin atc Ile ttc Ph e gcg Ala gtg Val1 45 tct Ser aac Asn at g Met aac As n atc Ile 125 Ctg Leu 9 ca Al a tg T rp ctc Le u ggt Gly 110 ttc Phe <210> 420 <211> 129 <212> PRT <213> Corynebacterium giutamicun <400> 420 Met Arg Val Val Leu Phe Leu Ala Val 1 5 Vai Thr Leu Ser Thr Thr Gly Asn Pro Ala Ala Gly Asp Ile Gly Leu Arg Ile 40 Ala Ser Ile Ser Ser Val Ile Gly Ala 55 Leu Val Giu Asn Lys Pro Glu Lys Lys 70 Ile Ile Phe Ilie Leu Ile Ser Cys Ser Ala Pro Ala Ile Leu Leu Val Phe Ala 100 105 Leu Pro Vai Gly Phe Thr Leu Met Ile 115 120 Lys tgg Trp aCC Th r gtg Val1 99C Gly 95 t tg Leu CgC Arg Le u Ala Phe Ser Arg ValI Gi y Tyr gca Ala ttc Phe aCC Thr 80 a Cg Th r gtC Val1 Caa Gin Gly Ala Gly Tyr Leu 75 Phe Ala Val gg Ala ctg Le u atC Ile gca Ala Ct C Leu aaa Lys ValI Giu Al a Thr Gin Ile Phe Ala tcc Ser qtg Val1 atC Ile cca Pro

CCC

Pro att Ile gaa Giu ttC Phe gca Ala gt a ValI 115 PCTA1B00100926 tCC tCa 259 Ser Ser aaC aag 307 Asn Lys atC Ctg 355 Ile Leu atC CtC 403 Ile Leu 100 ggc ttt 451 Gly Phe Val1 Ala Val1 Ser Asn Met Asn Ile 125 Gly His Ala Phe Thr Thr Val1 Gin <210> 421 WO 01/00805 <211> 490 <212> DNA <213> Corynebacteriur glutamicum <220> <221> CDS <222> (101)..(490) <223> FRXA01538 <400> 421 PCT/IBOO/00926 tctaaactca ctctcaactc accaagattg ttcaacaatc tgcgattggt gtgcaatcta ccccaatcat tttgaaagcc cccacgaaag gagcgcgaca atg gcc gac aac aaa ii.

Met Ala Asp Asn Lys gat Asp gac Asp gcc Al a tct Ser cag Gin ga c Asp atg Met ga c Asp att Ile t ct Ser 9CC Al a Ctc Le u atc Ile cgc Arg 105 agc Ser gca Al a ggc Gi y atc Ile Gly g cg Al a gCC Al a cag Gin aaa Lys cga Arg ggc Gi y gca Ala gt 9 Val1 caa Gin t ca Ser g cg Ala ctg Leu ttC Phe g ca Al a aat Asn gg C Gly 110 agc Ser tcc Ser ggt Gi y acc Thr gcg Al a tgg Trp a cg Th r gga Gly aag Lys atc Ile acc Th r Ctg Le u atc Ile atc Ile ggt tgg gtg ctg gCC gta ctg gtC tgt att ggc ggc gta Gly Trp Val Leu Ala Val Leu Val Cys Ile Gly Gly Val 120 125 130 <210> 422 <211> 130 <212> PRT <213> Corynebacterium glutarnicum (400> 422 Met Ala Asp Asn Lys Asn Ala Asp Asp Ser Gin Leu Val Ser Ala Ser 1 5 10 Thr Gly Thr Pro Gly Pro Gly Asp Ile Ala Lys Ala Asn Ala Pro Ser 25 WO 01/00805 Leu Lys Gin Ala Ala Val Thr Ala Ser Gly Arg 40 Ala Ile Phe Leu Met Ala Thr Ser Ala Ile Gly 55 Gin Thr Ala Val Phe Thr Asn Gin Leu Gly Ala 70 75 Ile Leu Val Ser Ile Leu Ilie Asp Ile Ala Val Arg Ile Ile Giy Val Ser Giu Met Arg Ala Gin 100 105 Vai Ilie Pro Gly Phe Gly Trp Val Leu Ala Val 115 120 Gly Val 130 <210> 423 (211> 1401 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1378) <223> RXA01727 (400> 423 agcttttggt ggtttcacca cctgcgctcg tcttaatttt tgaggagqgg ctgcgccgta tcgccagcgt gttgtaaata Ser Pro Al a Gin Gi u Leu Ala Gly Phe Le u Leu Val1 125 PCTIIBOO/00926 *Gly i Thr *Ala Trp Thr Gly gt c ValI aac Asn cta Le u Ct g Leu gct Al a tat Tyr act Th r ttc Ph e atc Ile atc Ile Gly atc Ile ct g Le u ctt Le u cta Leu ttg Leu ttt Phe tac Tyr ttt Phe ga Gi y 30 gca Ala t cg Se r ggC Gly g9cg Ala gcgtgttcca gagagaccct atg agt aaa aag tct Met Ser Lys Lys Ser 1 ttc ttc gga gct gga Phe Phe Gly Ala Gly gca gga acc aac tat Ala Gly Thr Asn Tyr gtt ctg ctc ccg gtg Val Leu Leu Pro Val aat gtc aag gac atg Asn Val Lys Asp Met ttt cct att gct gcc Phe Pro Ile Ala Ala agg act ggg 9cg gtg Arg Thr Gly Ala Val WO 01/00805 agc Ser ttt Phe aat Asn acg Th r 150 a cg Th r ggt Gly ttt Phe gt c Val1 g cg Al a 230 gag Glu atg Met tgt Cys aat As n gcg Ala 310 gcc Ala tat Tyr aac Asn ggc Gly 135 t tg Leu c ca Pro t tg Le u ggc Gly cgc Arg 215 ct g Leu ttc Phe g gt Gly atg Met tcg Ser 295 ctg Leu gt t Val1 t cg Se r ttt Phe 120 att Ile at t I le ggt Gly ctt Leu atc Ile 200 a cg Th r qt t Val1 gct Al a cag Gl n acc Th r 280 Ct g Leu att Ile g cg Al a acg gcg Thr Ala 105 gtg ttt Val Phe gca gac Ala Asp gtg gtg Val Val gag cca Glu Pro 170 gag ggc Glu Gly 185 gtg gtg Val Val gca act Ala Thr tat ctt Tyr Leu gat ggc Asp Gly 250 gct ggt Ala Gly 265 acc gca Thr Ala ctg cca Leu Pro tcc ttt Ser Phe gct cca Ala Pro 330 gtt ggc gtc Val Gly Val ga t Asp 110 gca Ala aag Lys t tg Leu tat Tyr atg Met 190 ttc Phe gcg Al a t ca Ser ttg Le u ttc Phe 270 atC Ile tac Tyr acc Th r ttc Phe aat gcg Asn Ala ctg gcg Leu Ala tgg ctc Trp Leu tcg gta Ser Val 160 gcg cag Ala Gln 175 gat gcg Asp Ala aag tac Lys Tyr ttc att Phe Ile atc ggt Ile Gly 240 aac tac Asn Tyr 255 gtg gct Val Ala agt gcg Ser Ala cac gtc His Val atg ggt Met Gly 320 att tac Ile Tyr 335 ctt tat tcg Leu Tyr Ser 115 ttg tcg tgg Leu Ser Trp 130 acg cca gcg Thr Pro Ala 145 gcc aag ttg Ala Lys Leu cag cct gcg Gln Pro Ala att gct gcg Ile Ala Ala 195 caa aag gtt Gln Lys Val 210 gcc gga att Ala Gly Ile 225 caa gta gta Gln Val Val gct gca ctg Ala Ala Leu att ttg atc Ile Leu Ile 275 acg tct gag Thr Ser Glu 290 tgg gcc act Trp Ala Thr 305 ttg gat acg Leu Asp Thr cca tcg gcc Pro Ser Ala PCTIIBOO/00926 100 ggc ttg 451 Gly Leu aat ccg 499 Asn Pro ttg ctc 547 Leu Leu gat ggc 595 Asp Gly 165 ggg gcg 643 Gly Ala 180 ttg gcg 691 Leu Ala aac aag 739 Asn Lys ttg ttg 787 Leu Leu aac ggt 835 Asn Gly 245 tcc acg 883 Ser Thr 260 ctt gca 931 Leu Ala ttt ttc 979 Phe Phe gtt ttc 1027 Val Phe gtg ttg 1075 Val Leu 325 atc acc 1123 Ile Thr 340 WO 01/00805 ttg gtg ttc ttg Leu Val Phe Leu 345 acc tac cta ttc Thr Tyr Leu Phe 360 tct atc cct gcg Ser Ile Pro Ala 375 atg tct ttg ggt Met Ser Leu Gly 390 ttg gct att gat Leu Ala Ile Asp gaa tcc att tcc Glu Ser Ile Ser 425 t cg Se r gg C Gi y ctg Leu t gg Trp t gg T rp 410 gtc Val1 ctc atc gag ccc ctg ctg ttc Leu Ile Giu Pro Leu Leu Phe 350 ait tgg act gca gtt gtg tgg Ile Trp Thr Ala Val Val Trp 365 aat cca ttc atc gaa tgg gcg Asn Pro Phe Ile Giu Trp Ala 380 385 qtt gtc cca gtt ctc gtg gcc Val Val Pro Val Leu Val Ala 395 400 aac aag aaa ggt gcc cag tct Asn Lys Lys Gly Ala Gin Ser 415 taatcgctaa ttgcgaggag tct cgt Arg gcg Al a 370 ccg Pro tct Ser gtt Val1 PCT/IBOO/00926 tgg 1171 Trp atg 1219 Met agc 1267 Se r ggt 1315 Gly 405 aag 1363 Lys 1401 <z210> 424 <211> 426 <212> PRT <213> Corynebacterium glutamicum <400> 424 Met Ser Lys Lys Ser Val Leu Ile Thr 1 5 Phe Phe Gly Ala Gly Asn Leu Ilie Phe Ala Gly Thr Asn Tyr Leu Pro Ala Ile 40 Val Leu Leu Pro Val Leu Ala Ile Ile 55 Asn Val Lys Asp Met Ala Ser Arg Gly 70 Phe Pro Ile Ala Ala Tyr Leu Ser Ile Arg Thr Gly Ala Val Ser Tyr Ser Thr 100 105 Leu Tyr Ser Gly Leu Phe Asn Phe Val 115 120 Leu Ser Trp Asn Pro Asn Gly Ile Ala 130 135 Thr Pro Ala Leu Leu Thr Leu Ile Val 145 150 Ser 10 Pro Leu Ala Gly Gly 90 Ala P he Asp Val Met Met Phe Val1 Ile Phe Gly Ala Le u 140 Val1 Leu Le u.

Leu Leu Phe Tyr Val1 Val 125 Gly Val Se r Le u Thr Gly Leu Leu Asn Le u T rp Ser Met Ser Ser Gi u Val1 Pro Ala Ala Leu Val1 160 WO 01/00805 Ala Lys Leu Gin Pro Ala Ilie Ala Ala 195 Gin Lys Val 210 Ala Gly Ilie 225 Gin Val Val Ala Ala Leu Ile Leu Ilie 275 Thr Ser Glu 290 Trp Ala Thr 305 Leu Asp Thr Pro Ser Ala Phe Arg Leu 355 Trp Ala Leu 370 Ala Pro Leu 385 Ala Ser Ala Ser Val Ala (210> 425 <211> 1461 <212> DNA Asp Gly 180 Leu Asn Leu As n Se r 260 Leu Phe Val1 Val Ile 340 Lys Phe His Ile Lys 420 Gly 165 Ala Al a Lys Leu Gly 245 Th r Ala Phe Phe Le u 325 Thr Trp Met Ser Gly 405 Lys Th r Gly Phe Val1 Al a 230 Giu Met Cys Asn Al a 310 Ala Le u Thr Ser Met 390 Le u Glu Pro Leu Gly Arg 215 Le u Phe Gly Met Ser 295 Leu Val1 Val Tyr Ile 375 Ser Ala Ser Giu Giu 185 Val1 Ala T yr Asp Al a 265 Thr Le u Ser Ala Le u 345 Ph e Ala Gly Asp Ser 425 Pro 170 Gly Val1 Thr Le u Gly 250 Gly Al a Pro Phe Pro 330 Se r Gly Le u T rp Trp 410 Val Ser T yr Ile Val1 Gi y 235 Th r Arg Val1 Gly Gly 315 Val Le u Ile As n Val 395 As n Se r Met Ser Val1 220 Leu Ala Ile Gi y Val1 300 Val1 Ile Ile T rp Pro 380 Val1 Lys Al a Th r Ala 205 Se r Gly Ile Met Leu 285 Lys Al a Se r Glu Th r 365 Phe Pro Lys Tyr Met 190 Ph e Ala Ser Le u Phe 270 Ile Tyr Th r Ph e Pro 350 Al a Ile Val1 Gi y PCTIBOO/00926 La Gin 3p Ala (s Tyr ie Ile Le Gly 240 3n Tyr al Ala !r Ala Ls Val t Gly 320 le Tyr au Leu al Val lu Trp au Val 400 l~a Gin <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1438) WO 01/00805 <223> RXA00623 <400> 425 tttaagcttc gtcggcaacg atcacaccac gttaaatttc PCTIBOO/00926 gaccatcaat gacggtggcc gttttttggc gaatccccaa aaagqtcgaa gaggaagttc atg gat tca aac aca Met Asp Ser Asn Thr gaa Giu ccg Pro gc a Al a aaa Lys att Ile gtc ValI tac Ty r ggt Gly ga a Giu ttc Phe 150 ggt Gly gct Ala cat His tct Ser ccg Pro ttg Leu gaa Glu atc Ile a ag Lys ttc Phe aac As n tct Ser 135 atc Ile t cg Ser ca g Gin ctg Le u agt Ser aag Lys ggC Gi y aa g Lys cca Pro gcg Al a a cg Tb r 105 at c Ile ttc Phe t cg Se r ct g Le u atg Met 185 aag Lys gtt ValI gac Asp ggt Gly att Ile gt t Val1 gca Ala atg Met cag Gin g ca Ala atc Ile ca g Gin 170 qgt Gly ctc Le u gt c ValI act Th r acc Thr 9gc Gly 60 ttc Phe gt c Val1 aca Tb r ggt Gly aca Thr 140 cct Pro c tg Le u a ag Lys ttC Phe gaa Glu 15 ctc Leu ctg Leu ttt Phe atc Ile g ct Al a 95 ctc Leu ctg Le u agc Ser ttc Phe gcc Ala 175 ccc Pro tt~g Le u cac His tac Tyr att Ile gtg ValI gt c Val1 80 ggt Gly g ca Al a aac Asn cct Pro ttc Phe 160 atc Ile atc Ile aac As n 1 att Ile att Ile tca Ser tcc Se r atc Ile ggc Gly gtt Val1 atc Ile gaa Gi u 145 tct Ser ctc Leu ct t Leu t gg Trp gtt Val1 gtc Val gag Giu atc Ile atc Ile gcc Al a ct g Le u 115 gtt Val1 ctc Le u ttt Ph e gg c Gi y ttc Phe 195 ctg Leu caa Gin att Ile t tg Leu aag Lys ggt Gly ctt Le u 100 cta Le u gat Asp ttg Leu act Thr ttt Phe 180 gta Val1 t gg T rp aag Lys atc Ile ggc Gly a tg Met t ca Ser g cg Ala qt c Val1 gaa Giu gga Gi y ga t Asp 165 g ca Ala tcc Ser ct c Leu 115 163 211 259 307 355 403 451 499 547 595 643 691 739 WO 01/00805 gcc cca gtc Ala Pro Val 215 ggc ttt gat Gly Phe Asp 230 gtc acc tgc Vai Thr Cys gtg ttc acc Vai Phe Thr gaa ttc ctg Glu Phe Leu 280 aac ctc atg Asn Leu Met 295 gga atc gtg Giy Ile Val 310 att tac ctc Ile Tyr Leu ccg atg aqc Pro Met Ser gca tcc aag Ala Ser Lys 360 qct gCC gga Ala Ala Gly 375 gtg att gtg Val Ilie Val 390 aac ttc gCC Asn Phe Ala ggc acc gtg Gly Thr Val cca ttt gtg Pro Phe Val 440 acg ggt Gi y gcc Ala gtg Val1 99 C Gi y 265 Ct 9 Le u cg c Arg gt c Val1 acc Thr Ct C Leu 345 gqC GI y t tg Leu ggC Gly gga Gly ga c Asp 425 gca Al a gtt Val1 atc Ile 250 gtg Val1 atc Ile aag Lys cca Pro atg Met 330 gg C Gly gct Ala tct Se r atc Ile aac Asn 410 atg Met qgt Gly 220 cag Gin atc Ile atc Ile gct Ala gaa Glu 300 gg C Giy tct Se r cag Gin gg t Gly cac His 380 aaa Ly s gt g Val caa Gin gca atg Ala Met ctc ggt Leu Gly ttt ggc Phe Gly ttc aag Phe Lys 270 acc tca Thr Ser 285 cac atc His Ilie tat tcc Tyr Ser atc ttc Ile Phe gtc ggt Vai Giy 350 gtc tcg Val Ser 365 cgc cca Arg Pro ttc atg Phe Met gca aca Ala Thr gtc cat Vai His 430 gac cac Asp His 445 gCC Al a att Ile gtg Val1 255 ctg Leu tcc Ser ggt Gly ttc Phe att Ile 335 ct g Le u ggt Gly gaa Glu tct Se r ct g Leu 415 gac ggc Gly ttg Leu 240 ct g Le u gtc Val1 tct Ser gt g Val1 aac As n 320 gcc Aila ctt Le u gcc Ala ctt Le u gaa Giu 400 ctg Le u gt t gtc Val1 225 atc Ile Gly aag Lays qaa Glu gct Aia 305 ttg Leu gac Asp gt C Val1 ggt Giy Ct g Leu 385 gcc Al a gtc Val1 ttg gtt Val1 ctc Leu gcc Ala tac Tyr tct Se r 290 aaa Lys ga c Asp gcg Ala ttc Phe att Ile 370 cac His cgc Arg ggc Gly aat PCT1BOO/00926 ggc gaa aca 787 Gly Glu Thr gcc ttt tac 835 Ala Phe Tyr 245 qta ctq aag 883 Val Leu Lys 260 ctt gcc aag 931 Leu Ala Lys 275 gCC ttg cca 979 Ala Leu Pro cca acc gtc 1027 Pro Thr Val ggc acc gca 1075 Gly Thr Ala 325 atg aat atg 1123 Met Asn Met 340 atg atc atc 1171 Met Ile Ile 355 gca acg ttg 1219 Ala Thr Leu ggc gtt gac 1267 Gly Val Asp gca cta acc 1315 Ala Leu Thr 405 aag tgg act 1363 Lys Trp Thr 420 gga aaa tct 1411 Gly Lys Ser 435 Asp Val Leu Asn gag tta gaa gaa Glu Leu Glu Glu tagttttcaa caggacqaca 1458 1461 WO 01/00805 WO 0100805PCT/iBOO/00926 <210> 426 <211> 446 <212> PRT <213> Corynebacterium glutamicum <400> 426 Met Asp Ser Asn Thr Giu Ser Ser Ser Val Giu Val Lys Asn Glu His 1 5 10 Ile Lys Val Gin Lys Pro Pro Lys Lys Asp Arg Thr His Trp Leu Tyr 25 Ile Ala Val Ilie Ile Ala Leu Ile Gly Gly Ile Thr Leu Gly Leu Ile 40 Ser Pro Giu Leu Gly Lys Giu Phe Lys Ile Leu Gly Thr Met Phe Val 55 Ser Leu Ile Lys Met Ile Ile Ala Pro Val Ile Phe Cys Thr Ile Val 70 75 Ile Gly Ile Gly Ser Val Lys Ala Ala Ala Thr Val Gly Arg Ala Gly 90 Gly Ile Ala Leu Ala Tyr Phe Ile Thr Met Ser Thr Phe Ala Leu Ala 100 105 110 Val Gly Leu Leu Val Gly Asn Phe Ile Gin Pro Gly Ser Gly Leu Asn 115 120 125 Ile Ser Val Asp Giu Giu Ser Ser Phe Ala Ser Thr Giu Ser Ser Pro 130 135 140 Giu Gly Leu Leu Gly Phe Ile His Ser Ile Ile Pro Giu Thr Phe Phe 145 150 155 160 Ser Ala Phe Thr Asp Gly Ser Val Leu Gin Val Leu Phe Ile Ala Ile 165 170 175 Leu Val Gly Phe Ala Ala Gin Ser Met Gly Giu Lys Gly Gin Pro Ile 180 185 190 Leu Asp Phe Val Ser His Leu Gin Lys Leu Ile Phe Lys Ile Leu Asn 195 200 205 Trp Ile Leu Trp Leu Ala Pro Val Gly Ala Phe Gly Ala Met Ala Gly 210 215 220 Val Val Gly Glu Thr Gly Phe Asp Ala Val Val Gin Leu Gly Ile Leu 225 230 235 240 Ile Leu Ala Phe Tyr Val Thr Cys Val Ile Phe Ile Phe Gly Val Leu 245 250 255 Gly Ala Val Leu Lys Val Phe Thr Gly Val Asn Ile Phe Lys Leu Val 260 265 270 Lys Tyr Leu Ala Lys Giu Phe Leu Leu Ile Phe Ala Thr Ser Ser Ser 275 280 285 WO 01/00805 WO 0100805PCTIBOO/00926 Se r 290 Lys Asp Ala Phe Ile 370 His Arg Gly As n Al a Pro Gly Met Met 355 Al a Gly Ala Lys Gly 435 Le u Th r Thr As n 340 Ilie Th r Val Le u Trp 420 Lys As n Gi y 310 Ile Pro Ala Ala Val1 390 As n Gly Pro Arg Val1 Th r Le u 345 Gly Leu Gly Gly Asp 425 Glu Lys Pro Met 330 Gly Ala Se r Ile As n 410 Met Le u Met Th r 315 Ala Glu Ala Se r Asp 395 Ser Asn Gl u His Tyr Ile Val1 Val1 365 Arg Phe Ala Val1 Asp 445 <210> 427 <211> 620 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (55)..(597) <223> RXA01584 <400> 427 ccaccccctt ccccgctacg cgccataccg gcttgggcgg gtg ttt ttg ggc gca ctt ctc ggc gcc Val Phe Leu Gly Ala Leu Leu Gly Ala 10 cca gca ttc att caa qcc gga tcc aca Pro Ala Phe Ile Gln Ala Gly Ser Thr 25 qtt ttg cca ctg ctg gaa aag ctc gtt Val Leu Pro Leu Leu Glu Lys Leu Val 40 gaa acc gac ttc cta tcc ggc tac tcc Glu Thr Asp Phe Leu Ser Gly Tyr Ser gt a Val1 gt g Val gtt Val1 gca Al a gcatcggatc actagtg Val1 1 atg ggc ggg ttt tat Met Gly Gly Phe Tyr ggc ggc ggc cac gtg Gly Gly Gly His Val ccc qgt ttt att aaa Pro Gly Phe Ile Lys cag gca gtg cct ggc Gln Ala Val Pro Gly 57 105 153 201 249 WO 01/00805 CCC atg tt Pro Met Ph.

ggt ggt qc.

Gly Gly Al ttg agc at Leu Ser Iii cgc atc ca Arg Ile Gl1 115 ttg ggc gc.

Leu Gly Al 130 gtt tcc gc.

Val Ser Al tgg aaa at' Trp Lys Iii tgg gtc tt Trp Val Le 18' c agc e Ser a gtg a Val c agc e Ser 0 a gca ni Ala a gcg a Ala a tta a Leu t ccg e Pro 165g Ctt u Leu 0 ttc Phe ct g Le u gga Gi y g ca Ala ctc Le u gct PAla 150 ccg 55 gcc Ala 9CC Ala atg Met qtc Val1 tac Tyr 135 atc Ile t gg tac ctc Tyr Leu ctg gcg Leu Ala 90 ttt tgg Phe Trp 105 ggc atc Gly Ile CCC gta Pro Val acg gtg Thr Val atc 9CC Ile Ala 99C Gly 75 atc Ile gga Gly aac Asn ttc Phe tqt Cys 155 9C 9 Ala 60 gca Ala ttC Phe C9 C Arg gCC Ala acc Th r 140 t gg Trp ggt Gly atc Ile tt C Phe t 99 T rp ggt Gly 125 cac His Ctg Le u 9 C9 Al a taC ggt ggC Tyr Gly Gly CCC 9CC gCa Pro Ala Ala Cga aaa gCa Arg Lys Ala 110 gtg 9tg 999 Val Val Gly ggC atC aCC Gly Ile Thr 999 Cta gCC Gly Leu Ala 160 9CC Ctt gca Ala Leu Ala CTJABOOIOO926 atC 297 Ile CtC 345 Le u CCg 393 Pro Ctt 441 Le u agc 489 Se r 145 CaC 537 His 99C 585 Gly Pro Trp 170 tagaaaacgc tcagacccaa aCC <210> 428 <211> 181 <212> PRT <213> CorynebaCteriun glutamiCum <400> 428 Val Val Phe Leu Gly Ala Leu Leu Gly 1 5 Tyr Pro Ala Phe Ile Gin Ala Gly Ser Val Val Leu Pro Leu.Leu Giu Lys Leu Lys Glu Thr Asp Phe Leu Ser Gly Tyr 55 Gly Pro Met Phe Ser Phe Ala Ser Tyr 70 Ile Gly Gly Ala Vai Leu Ala Ser Leu Leu Leu Ser Ile Ser Gly Met Tyr Phe 100 105 Ala Th r Val1 Se r Le u Ala Trp Ile Phe Ala Al a Al a Phe Arg Phe His Ile Pro Gly Ala Ala WO 01/00805 Pro Arg Ile Gin 115 Leu Leu Gly Ala 130 Ser Vai Ser Ala 145 His Trp Lys Ilie Giy Trp Val Leu 180 PCTIBOO/00926 Aia Ala Val Thr Giy Ile Asn Ala Giy Vai Vai Gly 120 125 Ala Leu Tyr Asp Pro Vai Phe Thr His Gly Ile Thr 135 140 Leu Aia Ile Ala Thr Val Cys Trp Leu Giy Leu Ala 150 155 160 Pro Pro Trp Aia Ile Ala Ala Gly Ala Ala Leu Ala 165 170 175 Le u <z210> 429 <211> 813 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (790) <223> RXA00852 <400> 429 agatcaccgt catggtcata atcgccgctg gagttcatgc agcattccct gacatccgta agaaatagtt ccaacccact tttcctcaga attgcagttc atg ccc gag atc att Met Pro Gu Ilie Ile ttt Phe cta Le u Gly cca Pro gga Gly aac As n act Th r aaa Lys aaa Ly s gg g Gi y gtt Val gtg Val1 gaa Glu ctg Le u tcC Ser cg t Arg cgc Arg atg Met cgc Arg gaa Glu ctc Leu cta Le u aag Lys act Thr tac Tyr caa Gin atc Ile gtt Val1 gt t Val1 gt g Val1 gac Asp 15 att Ile atg Met 9gc Gi y ttt Phe gag Glu 95 t cg Ser gg C Gi y atc Ile ctt Leu gt c Val1 80 ga t Asp 1 ctc Le u atc Ile aat Asn gac Asp ttc Ph e att Ile att Ile atc Ile ggt Gly gtC Val1 tct Se r gcc Ala tta Leu ggg Gly ctc Leu t cg Se r ga c Asp ttc Phe gag Glu gct Ala gg C Gly cat His gct Al a t cg Ser 100 ccc Pro aac As n gaa Giu tcc Ser gaa Glu ct t Le u gag Glu 115 163 211 259 307 355 403 451 cgc cgg cac aaa atg cca cgc gct gaa aag gcg caa cgt gtc gac Arg Arg His Lys Met Pro Arg Ala Glu Lys Ala Gin Arg Val Asp 105 110 115 WO 01/00805 atg atg gcg Met Met Ala 120 acc cta tcc Thr Leu Ser 135 att ttg gag Ile Leu Giu 150 gat ctg cgc Asp Leu Arg cag caa tta Gin Gin Leu gag cgg gtc Giu Arg Val 200 ccg caa aag cga Arg ggt Gly cca Pro aa t As n atc Ile 185 att Ile tcc cat His tta Le u ga t Asp ga c Asp 175 tta Leu aaa Lys t cg ga t Asp ctg Leu 145 ccc Pro ttc Phe ttC Phe gct Aila atg PCTJIBOO/00926 gc-ac 499 His a ctg 547 L. Leu 3 ctg 595 .~Leu 165 -gag 643 1 Giu

D

ttt 691 :Phe -cct 739 SPro Sgcg 787 Pro Gin Lys Ser Ile Asp Leu Tyr Vai Ser Leu Met Ala Giu Pro Ala 215 220 225 aaa tgaacagtat tcctttaggt ttt Lys 230 <210> 430 <211> 230 <212> PRT <213> Corynebacterium glutamicum <400> 430 *Met Pro Giu Ile Ile Phe Asp Asn Thr 1 5 Leu Ile Leu Giu Pro Leu Ser Leu Lys 25 Ile Ile Giy Aia Asn Giy Giy Gly Lys Asn Giy Leu Giy Glu Pro Thr Thr Gly Asp Val Ser His Ser Gly Arg Giu Val- 70 Phe Ser Asp Ala Giu Asn Gin Ile Val Ile Ala Phe Ser Leu Arg Arg His Lys 100 105 Glu 10 Leu Ser Arg Arg Met 90 Met Asp Arg Arg Asp Gly Arg Glu 110 Asp Ile Met Gly Phe Giu Lys Ser Gly Ile Le u Val Asp Ala WO 01/00805 Gin Arg Val Asp Giu Met Met Ala Arg Phe Asn 115 120 Asp Gin Ser Pro His Thr Leu Ser Giy Gly Gin 130 135 Leu Ala Ala Val Leu Ile Leu Giu Pro Giu Val 145 150 155 Pro Thr Thr Leu Leu Asp Leu Arq Asn Arg Leu 165 170 Phe Asn Lys Leu Glu Gin Gin Leu Ile Val Val 180 185 Phe Leu Ser Asp Phe Giu Arg Val Ile Cys Ile 195 200 Ala Ala Asp Giy Pro Pro Gin Lys Ser Ile Asp 210 215 Met Ala Giu Pro Ala Lys 225 230 <210> 431 <211> 1269 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1246) <223> RXA00690 <400> 431 atggatcatt ggactcacac tggctgtcat ttccttggtt gtgggcgatt cgtcgttcag aggtccagqq ttaaaqcttc Le u Lys 140 Ile Met Ser Asn Le u 220 PCTLBOO/00926 His Ala Leu Ala Asp Giu 160 Asp Val 175 Leu Asp Lys Ile Ser Leu cga Arg t gg T rp att Ile cgt Arg g cg Ala tat Tyr gcg Ala ttg Leu gg g Gly ggt Gly ctg Le u gg t Gly ctc Le u tcg Ser ct C Le u tcc Se r att Ile act Thr ccg Pro gtg Val1 tcagcgtctq ctgtggcggc gtg aaa tgg att gag Vai Lys Trp Ile Glu 1 tgg gcg tgg tgg gtg Trp Ala Trp Trp Val acc aac aat cct tat Thr Asn Asn Pro Tyr gtg gtg ttt aac cgt Val Val Phe Asn Arg atc tat ttg atg atc Ile Tyr Leu Met Ile cac att qtg gtg gga His Ile Val Val Gly 115 163 211 259 307 355 WO 01/00805 gca aaa att Ala Lys Ile ccg gaa tgg Pro Glu Trp ggt ctg atc Gly Leu Ile 120 gtg gcg gtq Val Ala Val 135 aaa tcg ttg Lys Ser Leu 150 ggt att tcc Gly Ile Ser cgt gca cga Arg Ala Arg gcg cgg att Ala Arg Ile 200 gcc ctg gct Ala Leu Ala 215 gta tcc aaa Val Ser Lys 230 ata ctc ggc Ile Leu Gly ccg atg ttc Pro Met Phe atc att tcg Ile Ile Ser 280 gat cag ttg Asp Gin Leu 295 gtg att ccg Val Ile Pro 310 ggc Gly gct Al a 105 atc I le gg t Gly cct Pro att Ile a cc Thr 185 ttg Le u aat Asn ttc Phe gt g Val gt t Val 265 ttg Le u cct Pro ctg Le u att Ile ggt Gly a cg Thr g cg Ala gca Ala 155 cct Pro cg t Arg C Cg Pro at g Met ca g Gin 235 gt t Val gt t Val1 gt t Val1 ggt Gly atg Met 315 gaa Giu atc Ile a cg Thr aac Asn 140 ttg Leu cag Gin ggt Gi y gtt Val1 gat Asp 220 cg t Ar g ggt Gly c cg Pro g ct Ala gct Al a 300 gct Ala Ctg Le u cac His caa Gin 125 t ct Se r ggc Gly atg Met gat Asp ttc Phe 205 gcc Ala gtg Val ctg Le u gtg Val1 tca Ser 285 gcg Ala gcg Ala ttt Phe gtg Val1 110 ggc Gly itg Leu gaa Glu gct Ala ga t Asp 190 ca g Gln cg t Ar g acc Th r ttt Phe ttt Phe 270 cat His gaa Giu ctq Leu cgg Arg ttt Phe tta Le u g cg Ala ctg Leu gag Giu 175 gcc Ala gac Asp ggt Gi y tct Ser gt g Val1 255 att Ile ag a Arg t gg Trp aca Th r c cg Pro gtg Val gga Gly 130 aag Lys gcg Ala ttc Ph e gtt ValI gat Asp 210 agg Arg ggt Gly gat Asp gtg Val1 tcc Ser 290 t gc Cys ct t Le u gtg Vali tat Tyr 115 a cg Thr aag Lys gtg Val cg c Arg cg t Arg 195 agg Arg cag Gin g ca Ala gca Ala gg c Gly 275 acc Thr atc Ile ga t Asp PCTIBOOOO926 cag ttg 403 Gin Leu 100 ctc gag 451 Leu Glu atg atc 499 Met Ile ctg ctc 547 Leu Leu gtc atc 595 Vai Ile 165 att aat 643 Ile Asn 180 ggt ttc 691 Gly Phe tct ttg 739 Ser Leu gct. cat 787 Ala His ttc gga 835 Phe Gly 245 tca tca 883 Ser Ser 260 ttc ttg 931 Phe Leu act ttt 979 Thr Phe aca ggt 1027 Thr Gly cca ggt 1075 Pro Gly 325 WO 01/00805 tcc atg atc acc acc tgg gtt cct1 Ser Met Ile Thr Thr Trp Val Pro 330 ttg ctc gtt gtg gca gga ctt gtt c Leu Leu Val Val Ala Gly Leu Val 345 acg ccc cgc ttg ccg aag aac aaa Thr Pro Arg Leu Pro Lys Asn Lys 360 365 ata aat agc cca gaa agg gcc gaa Ile Asn Ser Pro Giu Arg Ala Glu 375 380 9cg 'z210> 432 <211> 382 <212> PRT <213> Corynebacterium glutarnicum PCTIIBOO/00926 cat atg cca gac acc gtt ccg 1123 His Met Pro Asp Thr Val Pro 335 340 qcq acg atg cca gga ttc ttg 1171 Ala Thr Met Pro Gly Phe Leu 355 agg 9tc aag cgt cga aaa 9ca 1219 Arg Val Lys Arg Arg Lys Ala 370 taatgaqtgc tccttttagc 1266 1269 <400> 432 Vai Lys Trp Ile Glu Arg Tyr Val Leu Ser Arg Arg Met Val His Pro

I

Trp Th r Val1 Ile His Pro Th r Le u Pro 145 Th r Ala Trp Pro Asn Met ValI Gin 100 Leu Met Le u Val1 Ile 180 Val1 Tyr Arg Ilie Gly Le u Glu Ile Leu Ile 165 As n Al a Le u Gly Gly Lys Glu Le u Al a 135 Ser Ile Ala Ala Phe T rp Val 75 I le Gly Thr Ala Ala 155 Pro Arg Cys Thr Arg Arg Le u His Gin 125 Ser Giy Met Asp Ala Leu Ala Leu Phe ValI 110 Gly Le u Glu Ala Asp 190 Se r Cys Phe Val1 Arg Phe Le u Ala Leu Giu 175 Al a Met Phe Pro Met Ile Gi y Thr Asp Gly 160 Se r Lys WO 01/00805 Gly Val Arg 195 Leu Asp Arg 210 Gly Arq Gin 225 Phe Gly Ala Leu Asp Ala Gly Val Gly 275 Thr Ser Thr 290 Val Cys Ilie 305* Tyr Leu Asp Pro Asp Thr Met Pro Gly 355 Gly Se r Al a Phe Se r 260 Phe Th r Th r Pro Val 340 Phe Phe Le u His Gly 245 Ser Leu Phe Gly Gly 325 Pro Leu Met Se r Gin Thr 250 Ala Val1 T rp Le u Thr 330 Ala Pro Pro Met Gin 235 Val1 Val Val Gly Met 315 T rp Gly Lys Val1 Asp 220 Arg Gly Pro Al a Al a 300 Ala Val1 Leu Asn Phe 205 Ala Val1 Le u Val1 Ser 285 Ala Ala Pro Val1 Lys 365 Gin Arg Th r Phe Phe 270 His Giu Le u Le u Val1 350 Val PCTIIBOO/00926 Thr Tyr Ile 240 Val1 Th r Lys Le u Arg 320 Met Thr Val Lys Arq Arg Lys Aia Ilie Asn Ser Pro Giu Arg Ala Glu Vai 370 375 380 <210> 433 <211> 876 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101). .(853) <223> RXA00827 <400> 433 tgcgtgaatt aacagacaac ggaatcaqca ttgtgtcagt aacccatgat cctgatttca tcgcagcgct gggcgatcac cacattqagg tgagcgcgaa qtg aac ctq ctg atc Val Asn Leu Leu Ile 1 aaa att aat ccc gtc acc cgc atc atc gcg ttg atg gta ctg acc acg Lys Ilie Asn Pro Vai Thr Arg Ilie Ile Ala Leu Met Vai Leu Thr Thr 15 ccg ttg ctg ctg agt ttg gat gtg atq tcg gca gcg atc gcg ctg gtg Pro Leu Leu Leu Ser Leu Asp Val Met Ser Aia Ala Ile Ala Leu Vai 30 WO 01/00805 WO 0100805PCT/IBOO/00926 gca Ala cig Le u t cc Ser ctg Leu 999 Gly cqc Arg ctg Le u acg Thr 150 cgc Arg ttt Phe gcg Al a agg Arg gct Al a 230 acc Th r aaa Lys atg Met ctC Leu ctg Leu att Ile cct Pro 135 ctt Le u gga Gi y ggt Gl y atg Met gaa Giu 215 gcc Al a att Ile cgt Arg g cg Ala att Ile C9 t Arg gat Asp 120 ga a Glu ttt Phe at t Ile t tg Leu ga a Glu 200 tcc Ser att Ile att Ile 99 c Gly ct t Leu cac His.

gtt Val 105 .ccc Pro a gg Arq Arg gct Al a ttg Le u 185 gca Ala acc Thr t cc Se r ctg gca cca Leu Ala Pro ttt 9CC ggc acc tgg aag atg ctg Phe Ala Gly Val Thr Trp Lys Met Leu 45 t 99 Trp tat Tyr gtc Val1 ctg Le u acc Th r ttt Phe gaa Glu ga t Asp 170 gtg ValI cgc Arg gtc Val gcg Ala atg Met 9gc Gly 75 act Th r g cg Ala gac Asp gtc Val ga t Asp 155 cag Gin ctc Leu 99 t Gly 99 C Giy atc Ile 235 ctg Leu 60 a 99 Arg ga t Asp att Ile Ct 9 Le u atc Ile 140 t 99 Trp 199C Gly t Cg Se r ttt P he* Ala 220 gct ttc Phe Ccg Pro aat Asn g9t Gi y 99 C Giy 125 99 t Gly tac Tyr aag Lys ctt Le u 99 t Gly 205 C9 C Arg cta gca Ala aaa Lys gct Al a 9t t Val1 tt g Le u gca Al a tcc Ser 160 cac His 99 a Gly act Th r 9tg ValI tcc Ser 240 CC9 Pro gag Giu ttg Leu 9tg Val1 g9 Ala qga Gly 145 agg Arg ttt Phe tcC Ser 99 C Gly ctc Le u 225 att Ile gCt Ala ttt Phe gct Ala ctg Leu 115 Ct 9 Leu cqa Arg agg Arg a cc Thr ctt Leu 195 acc Thr ttg Leu act Thr 9 ca Al a a gc Ser 9CC Al a 100 att Ile Ct C Le u Ct 9 Le u cgt Arg at9 Met 180 gca Ala tgg Trp gt9 Vai ggt Giy Ala Leu Thr Val ttt aag ttc ttg Phe Lys Phe Leu 99C aca Giy Thr 250 tgatcacagt tttaattqat gqa <210> 434 <211> 251 <212> PRT WO 01/00805 <213> Corynebacterium giutamicun PCT/IBOO/00926 <400> 434 Val Asn Leu Leu Ile Lys Ile Asn 1 Met Al a Th r Pro Glu Leu Val1 Ala Gly 145 Arg Phe Se r Gly Leu 225 Ile Val1 Ile Trp Val Tyr Al a Val1 Gin 130 Se r Ala Phe Lys Arg 210 Ilie Gin Leu Al a Lys Al a Phe Al a Le u 115 Leu Arg Arg Th r Le u 195 Thr Le u Th r Th r Leu Met Al a Ser Ala 100 Ile Leu Leu Arg Met 180 Ala Trp Val1 Gi y 5 Thr Val1 Leu Le u Phe Ile Ala Lys Met Ala 165 Thr Thr Ala Cys Phe 245 Le u Thr Lys Met Le u Le u Ile Pro 135 Leu Gly Gly Met Glu 215 Ala Lys Leu Ile A rg Ala Ile Arg Asp 120 Glu Phe Ile Le u Glu 200 Se r Ile Phe Pro Val 10 Leu Ser Ile Leu Gly Trp Leu Tyr His Val 90 Val Leu 105 Pro Thr Arg Phe Arg Glu Ala Asp 170 Leu Val 185 Ala Arg Thr Val Ser. Ala Leu Gly 250 Ala Se r Gly Met Gly Leu Pro Gly Val1 Met Lys 175 Arg Th r Leu Val Leu Ala Val1 Ala Lys Ala ValI Le u Ala Ser 160 His Gly Thr Val1 Ser 240 <210> 435 <211> 732 <212> DNA <213> Corynebacteriui giutarnicum <2 <221> CDS <222> (101)..(709) <223> RXA00851 WO 01/00805 <400> 435 PCTIBOO/00926 ttgagcgggt catttgcatc aatgatcata aaatcgctgc tgatggccct ccgcaaaagt ccattgacct gtacgtatcg cttatggcgg aacctgcgaa atg aac agt att cct 11 Met Asn Ser Ile Pro tta Leu ttq Leu gcg Ala gtg Val1 ttg Le u t gg Trp t cc Ser g cg Ala ggc Gly ctc Le u 150 aaa Lys atc Ile ggt Gly t gg Trp gct Al a tt Ph e t gg Trp ca g Gin gcg Al a ctc LeU.

ctg Le u 135 att Ile gct Ala atc Ile tac Tyr ttc Phe acc Thr gtg Va 1 gtg Val1 gg C Gly atg Met 105 aat Asn gta Val1 ctg Leu ggt Gly t ca Se r 185 gt c Val cca Pro C C9 Pro ctg Leu ct g Leu ttt Phe gcc Ala gca Ala gag Giu caa Gin gcc Ala 170 ata Ile gat.

Asp ctt Leu gt t Val1 g cg Ala C cg Pro gat Asp gcc Ala gtt Val acg Thr t ta Le u 155 gg t Gly aag Lys 1 cat His atc Ile gtg Val gtc Val1 gg9t Gly gtg Val1 acc Th r cot Pro tct Se r 145 gtC Val ttt Phe gg C tct Ser 9 gC Gly ggg Gly g cg Ala g cg Al a ctc Leu acg Th r ttt Phe 130 ctc Le u ctc Leu gg C Gly gat ttt Phe ggc Gly att Ile t gg T rp ttt Phe a cg Thr cgg Arg 115 gca Al a acg Th r gat Asp acg Th r gct Ala Arg Ala Arg Asn Ile Gly Asp 190 taatttcttt caacacatag caa gca cgt ggt Ala Arq Gly 200 gcc ggc gat Ala Gly Asp WO 01/00805 <210> 436 <211> 203 <212> PRT <213> Corynebacterium glutamicum <400> 436 Met Asn Ser Ile Pro Leu Gly Phe Tyr PCTIIBOO/00926 1 His Ile Val1 Val Gly ValI Thr Pro Ser 145 Val P he Gly Se r Gly Gly Ala Ala Leu Th r Phe 130 Le u Leu Gly Asp 5 Ala Ile Val1 Gin Trp Phe Giu Phe Arg Arg 165 Val1 Leu Leu Ala Val1 Le u 70 Trp Ser Ala Gly Leu 150 Lys Ile Ala Trp Ala Phe 55 Trp Gin Al a Leu Le u 135 Ile Al a Ile Ar g Lys Ser Tyr Pro Arg Val1 Met 120 Pro Pro Arg Arg Gly 200 Val1 10 Pro Pro Le u Leu Phe 90 Al a Ala Giu Gin Ala 170 Ile Gly Asp Leu Val Ala Pro 75 Asp Ala Val Th r Le u 155 Gly Lys Asp Lys Le u His Ly s Ile Ph e Met Giu Ile 140 Ala Ph e Arg Gin Leu Gly Ile Le u Ala Leu Arg 125 Th r Thr Ser Ala Ser Phe Leu Pro Ile Ala Le u 110 Met Leu ValI Ile Arg 190 Val1 Ph e Ile Leu Met Th r Th r Leu Al a Lys Al a 175 As n Val1 Ile Le u Ly s Le u Th r Le u Gin Ile Ci u 160 Al a Ile <210> 437 <211> 725 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> <223> RXS03220 <400> 437 ccatg gqc tta agg gaa att ttg tcc agc aag tgg ctt gtg cgc atc ctc Met Gly Leu Arg Giu Ile Leu Ser Ser Lys Trp Leu Val Arg Ile Leu 1 5 10 WO 01/00805 Ctg gta ggt atc gga ttg ggt Leu Val Gly Ilie Giy Leu Gly tcc atc atg tac tac ggc cag Ser Ile Met Tyr Tyr Giy Gin gag aat gca gct ctg atc gcc Giu Asn Ala Ala Leu Ile Ala 55 gtc ggt gca ttc atc gca ctg Val Gly Ala Phe Ile Ala Leu 70 acc acc ctc att acc ggt tat Thr Thr Leu Ile Thr Gly Tyr atc ggt atc gca tcc gta gca Ile Gly Ilie Ala Ser Val Ala 100 tac gtt atc ttq act ctg gtt Tyr Val Ile Leu Thr Leu Val 115 ttC Ctc aac gta qct acc tgg Phe Leu Asn Val Ala Thr Trp 130 135 gca atg cqc qgt ttc gca atc Ala Met Arg Gly Phe Ala Ile 145 150 gca aac gcg ttc ctc gga ttg Ala Asn Ala Phe Leu Gly Leu 165 gga cta acc gga acc ttc ttc Gly Leu Thr Gly Thr Phe Phe 180 ttg atc ttc atc tac acc cag Leu Ile Phe Ile Tyr Thr Gin 195 gag gag att gat gag gat gtt Glu Giu Ile Asp Giu Asp Val 210 215 atc cga aaa gga aag gtg cac Ile Arg Lys Gly Lys Val His 225 230 gtc Val1 gt t Val1 aac Asn t gg T rp tct Se r ttc Phe gtg Val1 120 gtt Vali ggt Giy ttc Phe atg Met gtt Val1 200 act gca Al a gt t ValI gtg Val1 atg Met ctc Leu cca Pro 105 gt c Val1 atg Met atc Ile ttc Phe ttc Phe 185 cc t Pro tcc ca g Gin ctc Leu gcg Ala atg Met acc Thr 90 gt c ValI ttc Phe ctc Leu t ca Ser cca Pro 170 gcc Ala ga a Giu gg t cag Gin att Ile cca Pro gat Asp 75 acc Th r 9gc Gi y qt g Val1 tct Se r gt g Val1 155 acc Th r gg a Giy act Thr gtc ct g Le u gag Giu gga Gi y gg t Gly att Ile ga t Asp gga Gly gag Gi u 140 ttC Phe atc Ile atc Ile cgt Arg att atc Ile ttc Ph e gca Ala cgc Arg gta Val1 cgc Arg cag Gin C cg Pro t gq T rp gca Al a 175 gtt Val1 acc Thr aag PCTIBOOIOO926 aac 98 Asn tcc 146 Ser gtt 194 Val1 cgt 242 Arg ttg 290 Le u ccc 338 Pro acc 386 Thr ctg 434 Le u atc 482 Ile 160 gta 530 Val1 gcc 578 Ala ttg 626 Leu gac 674 Asp g 725 Thr Ser Gly Val Ile Phe Asn Lys taaaaaccca gacactgcat agataacac <210> 438 <211> 231 <212> PRT <213> Corynebacterium giutamicum WO 01/00805 WO 0100805PCTIIBOO/00926 <400> 438 Met Gly Leu Arg Giu Ilie Leu Ser Ser Lys Trp Leu Val Arg Ile Leu 1 5 10 Leu Val Gly Ile Gly Leu Gly Val Ala Gin Gin Leu Thr Gly Ile Asn 25 Ser Ile Met Tyr Tyr Gly Gin Val Val Leu Ile Giu Ala Giy Phe Ser 40 Glu Asn Ala Ala Leu Ile Ala Asn Val Ala Pro Gly Val Ile Ala Val 55 Val Gly Ala Phe Ile Ala Leu Trp Met Met Asp Gly Ile Asn Arg Arg 70 75 Thr Thr Leu Ile Thr Gly Tyr Ser Leu Thr Thr Ile Ser His Val Leu 90 Ile Gly Ile Ala Ser Val Ala Phe Pro Val Gly Asp Pro Leu Arg Pro 100 105 110 Tyr Val Ile Leu Thr Leu Val Val Val Phe Val Giy Ser Met Gin Thr 115 120 125 Phe Leu Asn Vai Ala Thr Trp Val Met Leu Ser Giu Leu Phe Pro Leu 130 135 140 Ala Met Arg Gly Phe Ala Ile Gly Ile Ser Val Phe Phe Leu Trp Ile 145 150 155 160 Ala Asn Ala Phe Leu Gly Leu Phe Phe Pro Thr Ile Met Glu Ala Val 165 170 175 Gly Leu Thr Gly Thr Phe Phe Met Phe Ala Gly Ile Gly Val Val Ala 180 185 190 Leu Ile Phe Ile Tyr Thr Gin Val Pro Giu Thr Arg Gly Arg Thr Leu 195 200 205 Glu Giu Ile Asp Glu Asp Val Thr Ser Gly Val Ile Phe Asn Lys Asp 210 215 220 Ile Arg Lys Gly Lys Val His 225 230 <210> 439 <211> 408 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(385) <223> FRXA02762 <400> 439 ttcccaqtcg gcgatcctct tcgcccctac gttatcttga ctctggttgt ggtcttcgtq WO 01/00805 ggatccatgc agaccttcct caacggtagc tacctgc ttc ccg ctg gca atg cgc ggt ttc Phe Pro Leu Ala Met Arg Gly Phe ctc tgg atc gca aac gcg ttc ctc Leu Trp Ile Ala Asn Ala Phe Leu gaa gca gta gga cta acc gga acc Glu Ala Val Gly Leu Thr Gly Thr 45 gtg qtt gcc ttq atc ttc atc tac Val Val Ala Leu Ile Phe Ile Tyr 60 cgt acc ttg gag gag att gat gag Arg Thr Leu Giu Glu Ile Asp Giu 75 aac aag gac atc cga aaa gga aag Asn Lys Asp Ile Arg Lys Gly Lys aga <210> 440 <211> <212> PRT <213> Corynebacterium giutamicum <400> 440 Met Leu Ser Giu Leu Phe Pro Leu 1 5 Ile Ser Val Phe Phe Leu Trp Ile Phe Pro Thr Ile Met Giu Ala Val Phe Ala Giy Ile Gly Vai Val Ala Pro Giu Thr Arg Gly Arg Thr Leu 70 Ser Giy Val Ile Phe Asn Lys Asp <210> 441 <211> 789 <212> DNA <213> Corynebacterium giutamicum ica ki a iga fly 30 :tc ?he ~cc C'hr a t ks p t g fa 1 PCTIBOOIOO926 3gtt atg ctc tct gag ctc 115 Met Leu Ser Glu Leu 1 ggt atc tca gtg ttc. ttc 163 Gly Ile Ser Val Phe Phe ttc ttc cca acc atc atg 211 Phe Phe Pro Thr Ile Met atg ttc gcc gga atc ggt 259 Met Phe Ala Gly Ile Gly gtt cct gaa act cgt gga 307 Val Pro Giu Thr Arg Gly act tcc qgt gtc att ttc 355 Thr Ser Gly Val Ile Phe 80 taaaaaccca gacactgcat 405 kla kla 25 fly eu ;i u Ile Ala Gly Phe Th r Asp Val1 Ile Le u Phe Gin Val1 His Gi y Phe Met Val1 Thr WO 01/00805 <s220> <221> CDS <222> (101) (766) <223> RXN00092 <400> 441 cgaaaccaga aacacccagc ggctcctcgt cgaaaagcga PCTIBOO/00926 atctttgaac tagaagccca gqcacgttgg ctcgaccgaa ttgaagcatt ggagcagtaa atg aca aac acg cct Met Thr Asn Thr Pro ttc Phe cgc Arg qaa Giu ctc Le u atc Ile acc Th r ccc Pro 99c Gly ctc Le u 9CC Al a 150 ttg Le u g ca Ala ctc ccc Pro ca C His ctc Leu aac Asn gac Asp a gg Arg a cc Thr aaa Lys g aa Glu 135 cag Gin ctt Leu gtc Val1 Ct t ctt Le u gt c Val1 gtt Val1 gt c Val1 ggt Gly cgt Arg ctc Leu acc Th r 120 ggC Gly cgc Arg gcc Al a ct C Le u gt C gaa Giu tcc Ser gcc Al a gcc Ala 9CC Ala cg C Arg acc Th r 105 ga C Asp ttc Ph e gtC Val1 ga C Asp cg C Arg 185 acc caa Gin Ct C Le u atg Met ct C Leu 9CC Ala 75 atc Ile gga Gly cag Gin ga C Asp atc Ile 155

CCC

Pro ctC Leu gaa aac atc Asn Ile aac aac Asn Asn ggc. ccg Gly Pro 45 cig cag Leu Gin tca gac Ser Asp gga gtt Gly Val gaa aac Glu Asn gca gta Ala Val 125 cgc ttc Arg Phe 140 gcg aga Ala Arg acc ggc Thr Gly cgc caa Arg Gin CCC CgC tgc C ys t cg Ser ggC Gly g ca Ala aac Asn ttc Phe ggt Gly atc Ile gaa Glu ctc Le u Ct C Leu 175 atc Ile gcc gga Gly gt c ValI aaa Lys ggc Gi y cg C Arg tac Tyr cta Le u g9cg Ala 130 tCC Se r

CCC

Pro t CC Se r gg t Gly g ca gaa Glu aat As n tcc Ser cat His gca Ala aac As n gaa Glu 115 gaa Glu ggc Gly cga Arg acc Th r gcc Ala 195 ga c ggC Gi y ccc Pro acc Th r gtg Val1 gct Al a ctg Leu 100 ctc Leu gtc Val1 9gc Gly aaa Lys ggc Gly 180 gca Al a cga cca Pro ggc Gly t tg Le u ctt Le u gaa Giu gtc ValI ga c Asp ggc Gly ca a Gin atc Ile 165 ga c Asp ggc Gly aca 115 163 211 259 307 355 403 451 499 547 595 643 691 739 WO 01/00805 PCTIBOOIOO926 Leu Leu Val Thr His Glu Pro Arg Phe Ala Ala Trp Ala Asp Arg Thr 200 205 210 atc atg ctt agg gat ggt gaa atc cag tgaccacact tctagcagca 786 Ile Met Leu Arg Asp Gly Giu Ile Gin 215 220 acc 789 <210> 442 <211> 222 <212> PRT <213> Corynebacterium glutamicum <400> 442 Met Thr Asn Thr Pro Phe Pro Leu Glu 1 Phe Ala Gly Se r Lys Asn Pro Le u Ile 145 Gly Thr Ser Gly Val1 Lys Gly Arg Tyr Le u Al a 130 Se r Pro Se r Gly Arg Glu Leu Ile Thr Pro Gly -Le u Al a 150 Leu Ala Le u His Leu Asn 55 Asp Arg Thr Lys Glu 135 Gin Le u Val1 Leu Met 215 Val Val1 Val Gly Arg Le u Thr 120 Gly A rg Ala Leu Val1 200 Se r Al a Ala Ala Arg Th r 105 Asp Phe Val Asp Arg 185 Th r Asn Asn Gly Le u Se r Gly Glu Ala Arg 140 Ala Th r Arg Pro Ile Asn Pro Gin Asp Val As n Val1 125 Phe Arg Gly Gin Arg 205 Se r Val1 Se r Arg Le u Ile ValI 110 Ala Pro Ala Ala Arq 190 Phe Trp Ala 210 Asp Arg Thr Ile Leu Arg Asp Gly Giu Ile Gin <210> 443 <211> 227 WO 01/00805 PCTJLBOO/00926 (212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> <223> FRXA00092 <400> 443 atc gcg aga gcc ctc atc ggc ccc cga aaa atc ttg ctt gcc gac gaa 48 Ile Ala Arg Ala Leu Ile Gly Pro Arg Lys Ile Leu Leu Ala Asp Giu 1 5 10 ccc acc ggc gcc ctc gac acc tcc acc ggc gac gca gtc ctc cgc gtc 96 Pro Thr Gly Ala Leu Asp Thr Ser Thr Gly Asp Ala Val Leu Arg Val 25 ctc cgc caa aga atc gat tcc ggt gcc gca ggc ctc ctt gtc acc cac 144 Leu Arg Gin Arg Ile Asp Ser Gly Ala Ala Gly Leu Leu Val Thr His 40 gaa ccc cgc ttc gcc gcg tgg gca gac cga aca atc atg ctt agg gat 192 Giu Pro Arg Phe Ala Ala Trp Ala Asp Arg Thr Ile Met Leu Arg Asp 55 ggt gaa atc cag tgaccacact tctagcagca acc 227 Gly Giu Ile Gin <210> 444 <211> 68 <212> PRT <213> Corynebacterium giutamicum <400> 444 Ile Ala Arg Ala Leu Ile Gly Pro Arg Lys Ile Leu Leu Ala Asp Glu 1 5 10 Pro Thr Gly Ala Leu Asp Thr Ser Thr Gly Asp Ala Val Leu Arg Val 25 Leu Arg Gin Arg Ilie Asp Ser Gly Ala Ala Gly Leu Leu Val Thr His 40 Giu Pro Arg Phe Ala Ala Trp Ala Asp Arg Thr Ile Met Leu Arg Asp 55 Gly Giu Ile Gin <210> 445 <211> 975 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <223> RXN03060 WO 01/00805 <400> 445 tatgtccttt ggctccggta ccctggcagg agtacctggg PCT/IBOO/00926 ctgtttttct aaaatggcct gacgttttca agattgaatt taaggaaagc atcgtagttc atg agt aac cct gcc 9 C9 Ala ga c Asp aat Asn gcg Ala gtg Val tcg Se r act Thr gac Asp cag Gin ctg Leu 150 gag Glu g ca Ala ttg Le u ttg gcc Ala a cg Thr 9gt Gly ggt G ly aaq Lys a cg Thr qca Ala acc Thr ttg Le u atc Ile cgt Arg 170 9gt Gly ccg Pro tct aa c As n ccg Pro ga a Gi u ga t Asp aac As n 75 ttg Le u ttc Ph e tct Se r gt t Val1 gcg Al a 155 ctg Leu ggt Gly gag Glu tct gt t Val1 ga c Asp gca Ala gcg Ala atc Ile 80 gct Ala ga t Asp cg t Arg g cg Ala ga t Asp 160 cg C Arg gtg Val1 ga c Asp ga t Met 1 gcg Ala t ct Ser gtg Val1 ttg Le u atg Met gg t Gi y Ct 9 Le u ttg Leu t cg Se r 145 cag Gin ctt Le u gcg Ala gag Glu atc aag Ly s tCC Se r aac As n ga t Asp gg t Gly ctg Le u tcg Se r gga Gly 130 gag Glu tcg Se r aag Lys tgt Cys cca Pro 210 ctg Ser Asn Pro Ala gag Glu cag Gin ctg Le u ca c H is CC t Pro ga t Asp Cgg Arg 115 ttc Phe aac Asn t gg T rp cac His gc t Ala 195 acc Thr cg C aat As n CCt Pro tac Tyr gtc Val1 t Ct Se r gct Al a 100 ttg Le u att Ile att Ile ttc Phe cg t Arg 180 cg t Arg ggt Gly acc tgg Trp gtc ValI aag Lys aac As n ggc Gly gcg Al a aa 9 Lys ttc Phe acg Th r ga t Asp 165 c~t Pro gcg Al a aac As n gca WO 01/00805 Leu Asp Ser 215 gtt gat cag Val Asp Gin 230 gcg gcg tcc Ala Ala Ser gtg aac cag Val Asn Gin aac gga att Asn Gly Ile 280 Ser cag Gin 235 gat Asp ga t Asp att Ile Giu Val Leu Asp Ile Leu Arg Thr 225 gtt gtg atc gtt acc cac gat gcc Val Val Ile Val Thr His Asp Ala 240 gtc att ttc ttg gcg gac ggt cgt Val Ile Phe Leu Ala Asp Gly Arg 255 260 acc atc gag gaa atc ttg gcc acg Thr Ile Giu Gu Ile Leo Ala Thr 270 275 taatgaattc cggttccaca atg PCTIBOO/00926 Ala aag 835 Lys 245 atc 883 Ile atg 931 Met 975 <210> 446 <211> 284 <212> PRT <213> Corynebacterium giutamicum <400> 446 Met Ser Asn Pro Ala Ala Ser Thr Pro Lys Se r As n Asp Gi y Leo Se r Gly 130 Glu Ser Ly s Trp Vali Lys As n Gly Al a Lys Phe Th r Asp 165 Pro Asp As n Al a ValI Ser Thr Asp Gin Le u 150 Glu Al a Se r Se r Gly Phe Lys Giy Glu 120 Phe Thr Thr Leu Phe Th r Gin Giu Se r Ser 105 Met Asn Asp Se r Se r 185 Al a 10 Th r Gly Giy Lys Th r 90 Ala Th r Leo Ile Arg 170 As n Lys Al a Th r Lys Met Ile Leo Pro 140 Gly Gly Ser Th r Ala Th r Ph e His Gi y Arg 125 Thr Arg Leu Asp Asp Al a Val Th r Cys Asp 110 Arg Leo Lys Th r Asp 1 Ile Arg Th r Ala Met Thr Asp Thr Ile Glu 175 Val1 Asp Al a Ala Ile Al a Asp Arg Ala Asp 160 Arg Gly Giy Gin Gin Gin Arg Val 190 WO 01/00805 WO 0100805PCTIIBOO/00926 Cys Pro 210 Leu His Asp Leu Ala 195 Thr Arg Asp Gly Al a 275 Leu Leu Val1 230 Ala Val1 As n Val1 Asp 215 Asp Ala Asn Gly Ser 200 Se r Gin Se r Gin Ile 280 Phe Gly Val Leu Val Ile Ile Phe 255 Ile Giu 270 <210> 447 <211> 538 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (538) <223> FRXA02618 <400> 447 cgaaaccaga aacacccagc ggctcctcgt cgaaaagcga ggcacgttgg ctcgaccgaa ttgaagcatt ggagcagtaa ttc Phe cgc Arg ga a Glu ctc Le u atc Ile acc Th r ccc Pro cac His ctc Le u aac As n gac Asp agg Arg ctt Le u 9 cg Ala atc Ile Gly agc Se r cac His caa Gin ctc Le u atg Met ctc Le u

C

Al a 75 atc Ile atctttgaac tagaagccca atg aca aac acg cct Met Thr Asn Thr Pro 1 ttc gga gaa ggc cca Phe Giy Glu Gly Pro gca gtc aat ccc ggc Ala Val Asn Pro Giy gga aaa tcc acc ttg Gly Lys Ser Thr Leu tct ggc cat gtg ctt Ser Gly His Val Leu aaa cgc gca gct gaa Lys Arg Ala Ala Glu aac tac aac ctg gtc Asn Tyr Asn Leu Val 100 WO 01/00805 CCC aCC ctc acc gtC gga gaa aac Pro Thr Leu Thr Val Gly Giu Asn 105 ggc aaa acc gac cgc cag gca gta Gly Lys Thr Asp Arg Gin Ala Val 120 125 ctc aag ggc tct acg acc gct ttt Leu Lys Gly Ser Thr Thr Ala Phe 135 140 <210> 448 <211> 146 <212> PRT <213> Corynebacterium giutamicum <400> 448 Met Thr Asn Thr Pro Phe Pro Leu 1 5 Phe Gly Glu Gly Pro Arg His Val Ala Val Asn Pro Gly Glu Leu Val 40 Gly Lys Ser Thr Leu Leu Asn Val Ser Gly His Val Leu Ile Asp Gly 70 Lys Arg Ala Ala Glu Thr Arq Arg Asn Tyr Asn Leu Val Pro Thr Leu 100 Pro Leu Glu Leu Asp Gly Lys Thr 115 120 Leu Ala Giu Val Gly Leu Lys Gly 130 135 Ser Leu 145 <210> 449 <211> 975 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (952) <223> FRXA02900 <400> 449 3tc Ile 110 g Ca Pro Glu Ser 25 Al a Ala Ala Arg Thr 105 Asp Ser PCTJIB0O/00926 ggt ctg ccc cta gaa ctc gac 451 Gly Leu Pro Leu Giu Leu Asp 115 atc gca ctc gcg gaa gtc ggc 499 Ile Ala Leu Ala Glu Val Gly 130 gag aga tct ctg 538 Giu Arg Ser Leu 145 Le u 10 Al a Ile Gi y Ser His 90 Val1 Ar g Th r Gln Leu Met Leu Ala 75 Ile Gly Gin Th r Ile Asn Pro Gin Asp Val1 As n Val1 125 Phe Ser Val1 Ser Arg Leu Ile Ile 110 Ala Pro Cys Ser Ci y Ala As n Phe Gi y Ile Giu Ala Le u Ser Th r Ala Gin Leu Ala Arg WO 01/00805 tatgtccttt ggctccggta ccctggcagg agtacctggg PCTIBOOIO0926 ctgtttttct aaaatggcct gacgttttca agattgaatt taaggaaagc atcgtagttc atg agt aac cct Met Ser Asn Pro gcg agc Ala Ser gac tct Asp Ser aat aac Asn Asn gcg tat Ala Tyr gtg gag Val Glu tcg ggt Ser Gly act ggt Thr Gly gac aaa Asp Lys cag teg Gln Ser 135 ctg cct Leu Pro 150 gag att Giu Ile gca gag Ala Glu ttg gtg Leu Val ttg gat Leu Asp cct Pro ttt Phe act Thr cag Gin gag Giu tcc Se r t cg Ser 105 atg Met aac Asn gat Asp t ct Ser tct Ser 185 cg t Arg gcc Al a acg Thr gg t Gly ggt Giy aag Lys acg Th r gca Ala acc Th r ttg Leu atc Ile cg t Arg 170 ggt Gly ccg Pro aac As n Ccg Pro ga a Glu gat Asp aac As n 75 t t~g Leu ttc Phe t ct Se r gt t Val1 gcg Ala 155 ctg Le u ggt Gly gag Glu aat As n a ag Lys gcc Ala acc Thr 60 a ag Lys atg Met att Ile ttg Le u cct Pro 140 ggc Gly gg t Gly ca g Gin atc Ile tcg gac gat Ser Asp Asp 15 act gac att Thr Asp Ile 30 gct gcg cgc Ala Aia Arg act gtc acc Thr Val Thr ttc act gcc Phe Thr Aia cac tgc atg His Cys Met 95 ggt gat acg Gly Asp Thr 110 cgt cgt gat Arg Arg Asp 125 act ctg acg Thr Leu Thr cgc aag att Arg Lys Ile ctg act gag Leu Thr Glu 175 cag cag cgt Gln Gln Arg 190 att ttc ggc Ile Phe Gly 205 gaa gtg ctg Glu Val Leu 1 gtt gcg Val Ala gac tct Asp Ser gca gtg Ala Val gcg ttg Ala Leu atc atg Ile Met 80 gct ggt Ala Gly gat ctg Asp Leu cgt ttg Arg Leu gcg tcg Ala Ser 145 gat cag Asp Gin 160 cgc ctt Arg Leu gtg gcg Val Ala gac gag Asp Glu gat atc Asp Ile 225 a ag Lys tcc Ser aac Asn gat Asp gg t Gly ct g Le u tcg Ser gga Gly 130 gag Gi u t cg Se r aag Lys tgt Cys cca Pro 210 ctg Le u gag Glu ca g Gin ct g Leu cac His cct Pro ga t Asp Cgg Arg 115 ttc Phe aac Asn t gg T rp cac His gct Ala 195 acc Th r cgc Arg aat Asn cct Pro t ac Tyr gt c Val1 tct Ser gct Ala 100 ttg Leu att Ile att Ile ttc Phe cg t Arg 180 cg t Arg gg t Gly acc Th r gcc Aia t gg Trp gt c ValI aag Lys aac As n gg c Giy gcg Aila aa g Lys ttC Ph e acg Thr ga t Asp 165 cct Pro g cg Ala aac Asn g ca Ala 115 163 211 259 307 355 403 451 499 547 595 643 691 739 787 tcg aac tct tct agg Ser Asn Ser Ser Arcs WO 01/00805 gtt ValI 230 gcg Al a gtg Val aa c As n cag Gin 235 gat Asp gat Asp att Ile acc Thr cg t Arg ccc Pro g cc Ala PCTIBOOIOO926 gtt gtg atc gtt acc cac gat gcc aag 835 Val Val Ile Val Thr His Asp Ala Lys 240 245 gtc att ttc ttg gcg gac ggt cgt atc 883 Val Ile Phe Leu Ala Asp Gly Arg Ile 255 260 acc atc gag gaa atc ttg gcc acg atg 931 Thr Ile Giu Giu Ile Leu Ala Thr Met 270 275 taatgaattc cggttccaca atq 975 <210> 450 <211> 284 <212> PRT <213> Corynebacterium giutamicui <400> 450 Met Ser Asn Pro Ala Ala Ser Thr Pro Ala Asn Asn Ser Asp Asp Val 1 Ala Lys Giu Asn Ser Ser Gin Pro Val Asn Leu Tyr Leu Asp His Val Met Gly Pro Ser Gly Leu Asp Ala 100 Leu Ser Arg Leu 115 Leu Gly Phe Ile 130 Ser Giu Asn Ile 145 Gin Ser Trp Phe Leu Lys His Arg 180 Ser As n T yr Glu Gly Gly Lys Ser 135 Pro Ile Glu Se r Se r Gi y Phe Lys Gly Giu 120 Phe Thr Thr Leu 10 Phe Thr 25 Thr Gly Gin Gly Giu Lys Ser Thr Ser Ala 105 Met Thr Asn Leu Asp Ile Ser Arg 170 Ser Gly 185 Arg Pro Pro Glu Asp As n 75 Le u Phe Se r Val1 Al a 155 Le u Gi y Glu Asp Ala Val1 Thr Cys Asp 110 Arg Leu Lys Thr Gin 190 Phe Ile Arq Th r Ala Met Thr Asp Th r Ile Giu 175 Arg Gly Asp Al a Al a Ile Aila Asp Arg Ala Asp 160 Arq Val Asp Aia Cys Ala Arg Ala Leu Val Ser

GJ

I]

Al 11 WO 01/00805 195 Lu Pro Thr 210 Le Leu Arg *r His Asp Asp Gly *e Leu Ala 275 Gly Asn Thr Ala Ala Lys 245 Arg Ile 260 Thr Met PCT/IBOO/00926 Leu Asp 215 Val Asp 230 Ala Ala Val Asn Asn Gly 200 205 Ser Asn Ser Ser Arg Giu Vai Leu 220 Gin Asp Asp Gin Thr Val Val Ile 235 Ser Tyr Ala Asp Arg Val Ile Phe 250 255 Gin Leu Phe Asp Pro Thr Ile Giu 265 270 Ile Glu Asp Ile Ala 280 <210> 451 <211> 1452 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (1)..(1452) <223> RXS03212 <400> 451 gcc tca ctt aac t Ala Ser Leu Asn T 1 gcg aca gtg gtg t Ala Thr Val Val T gct agt tct gcg t Ala Ser Ser Ala L att ttg ttt ggc a Ile Leu Phe Gly T agt aaa ttc ggc a Ser Lys Phe Gly T ttt cgc acg gtg t Phe Arg Thr Val S att ggt ttg atg t Ile Gly Leu Met P 100 tca gtc atc gtt cca gcc cta gtc att gtc cta Ser Val Ile Val Pro Ala Leu Val Ile Val Leu 10 gg t Gly t ca Se r gtc Val at t Ile t gg Trp tac Tyr ttt Phe ggC Gly gtt Val gaa Giu g ca Ala acc T hr acc Thr tgg T rp atc Ile gca Ala ggt Giy ttc Phe 110 aat ggt gta Asn Gly Val 115 cct gga cat gat gaa cac aat gtt ggc gtt gct atg tcc Pro Gly His Asp Giu His Asn Val Gly Vai Ala Met Ser WO 01/00805 acg aca atg ttc Thr Thr Met Phe 130 gtg ggc cta gcc Val Gly Leu Ala 145 ctt cta agc tct Leu Leu Ser Ser gga tgg ttg ggc Giy Trp Leu Gly 180 ttc ggc acg gca Phe Gly Thr Ala 195 g9c ctg tcc gca Gly Leu Ser Ala 210 gtt ggc att gtt Val Gly Ile Val 225 tct ggt gtg ggc Ser Gly Val Gly ctg gca gct ctg Leu Ala Ala Leu 260 tcg att ttg aac Ser Ilie Leu Asn 275 ttc ttt caa atg Phe Phe Gin Met 290 ggt gag tgg cta Gly Glu Trp Leu 305 tct tgg tca cca Ser Trp Ser Pro cgc tcc atc cgt Arg Ser Ile Arg 340 gtg tcc acc gta Val Ser Thr Val 355 gaa caa aat ggg ca c His att Ile g ca Ala 165 a ag L ys tgt Cys gca Ala tct Ser aag Lys 245 ctc Le u ct g Le u gca Al a gg t GI y ttc Phe 325 gag Glu t gg T rp gaa tgg T rp gcc Al a 150 ttc Ph e Ct C Leu tcc Ser aac Asn gtt ValI 230 gga Giy Ala ct 9 Le u ggc Gly a gc Ser 310 gta Val1 ttc Phe ttc Phe tcC acc Thr 135 tat T yr gtg Val1 atc Ile ctt Leu atc Ile 215 ttg Le u atc Ile att Ile cca Pro Cgc Arg 295 tgg T rp gga Gly atc Ile tcc Ser att ttg Le u t cg Ser cca Pro ga c Asp ggc Gi y 200 att Ile acc Thr cag Gin ttC Phe ggt Gi y 280 act Thr acc Thr atg Met ctg Le u att Ile 360 t gg cca Pro ttc Phe att Ile 170 ctg Leu 99 t Gly gat Asp 9 ca Al a ct C Leu 250 ttC Phe att Ile atg Met ttc Ph e t tg Le u 330 gtg Val1 ggt Gly gat tgg Trp cga Arg 155 gg t Gi y 9 c9 Ala gcc Ala C ca Pro ttt Phe 235 tcc Se r gtt Val1 Gly agt Ser ta c Tyr 315 gca Al a ttg Le u ggc Giy 99 t gct Al a 140 gtg Val1 ga a Glu att Ile ct g Leu agc Ser 220 atc Ile aac Asn gtc Val1 aac As n gcc Ala 300 t 99 T rp cgt Arg ctc Le u act Th r gca atc Ile 9gC Gly aaa Lys atc Ile cag Gin 205 gat Asp ttc Phe

C

Al a gga Gly tac Tyr 285 ga c Asp gca Ala att Ile 9t C Val1 Al a 365 gca PCT/IBOO/00926 gca att 432 Ala Ile aaa cag 480 Lys'Gin 160 gca gaa 528 Ala Glu 175 acc gta 576 Thr Val ggt gca 624 Gly Ala acc atc 672 Thr Ile gct att 720 Ala Ile 240 atg gtt 768 Met Val 255 acc gtg 816 Thr Val tcc aac 864 Ser Asn aca gca 912 Thr Ala tgg atc 960 Trp Ile 320 cgt ggC 1008 Arg Gly 335 gca ggt 1056 Ala Gly gtc ttc 1104 Val Phe gag cag 1152 WO 01/00805 Glu Gin Asn 370 ctt itt gga Leu Phe Gly 385 atc gcc atg Ile Ala Met gct icc acc Ala Ser Thr aac aag igg Asn Lys Trp 435 cta acg cia Leu Thr Leu 450 aac gic acc Asn Val Thr 465 ttg atg tt Leu Mei Phe Gly i ig Le u at Ile gi c Val1 420 gig Val1 t 9 Le u atc Ile gcg Al a Giu ct Leu ii 9 Le u 405 atg Met aca Th r cii Leu gig Val1 Ile 375 gca Al a 9gt Gly acc Th r gcc Al a ggi Gly 455 gca Ala Trp ct Leu act Th r aig Met t gg Trp 440 gg t Gi y aca Th r Gly cca Pro tic Ph e agt Ser 425 gg i Gly gac Asp cca Pro Asp ggt Gly tic.

Phe 410 cag Gin gt Val1 aa i As n tc Phe PCTIBOO/00926 Gin aic 1200 Ile 400 ici 1248 Ser gcc 1296 Ala 99a 1344 Gly caa 1392 Gin gga 1440 Gly 480 1452 <210> 452 <211> 484 <212> PRT <213> Corynebacierium glutamicun <400> 452 Ala Ser Leu Asn Trp Ser Val Ile 1 5 Ala Thr Val Val Trp Gly Ile Gly 1 Ala Ser Ser Ala Leu Ser Ala Val Ile Leu Phe Gly Thr Val Phe Val1 Ser Lys Phe Gly Thr Ile Arg Leu 70 Phe Arg Thr Val Ser Trp Ile Ser 1 Ile Gly Leu Mei Phe Tyr Gly Thr 100 Asn Gly Val Pro Gly His Asp Giu 115 120 Pro Lys Asp Ph e Arg Met Giu Asn Al a Asp Asn Ile Ile Ph e Pro Val WO 01/00805 PCTIIBOO/00926 Phe His Trp Thr Leu His Pro Trp Ala Ile Tyr Ala Ile Th r Val1 145 Leu Gly Phe Gly Val 225 Ser Le u Ser Phe Gly 305 Ser Arg Val1 Giu Le u 385 Ile Ala As n Thr 130 Gi y Leu Trp Gly Leu 210 Gi y Gi y Al a Ile Phe 290 Glu Trp Se r Ser Gin 370 Phe Ala Ser Lys Met Le u Se r Le u Thr 195 Ser Ile Val1 Ala Le u 275 Gin T rp Ser Ile Thr 355 Asn Gly Met Thr Trp 435 135 140 Ala Ser Gly 180 Al a Ala Val1 Gly Le u 260 As n Met Le u Pro Arg 340 Val1 Gly Le u Ile Val1 420 Val1 Ile Ala 165 Lys Cys Ala Se r Lys 245 Leu Leu Al a Gly Phe 325 Glu Trp Giu Leu Le u 405 Met Th r Arg 155 Gly Ala Ala Pro Phe 235 Ser Val1 Giy Ser T yr 315 Ala Leu Gly Gly Gly 395 I i,e His Ala Val1 Giu Ile Le u Se r 220 Ile As n Val1 As n Ala 300 Trp Arg Le u Th r Al a 380 Gin Th r Gly Th r Gly Lys Ile Gln 205 Asp Phe Ala Gly Tyr 285 Asp Ala Ile Val1 Al a 365 Ala Ile Ser Gin Ala 445 Arg Gi y Ala 190 Ile Trp Ser Asn Pro 270 Le u Gly Trp Ser Pro 350 Ile Glu Met Ala Leu 430 Ala WO 01/00805 PCT/IBOO/00926 Leu Thr Leu Leu Leu Ser Gly Gly Asp Asn Ala Leu Ser Asn Leu Gin 450 455 460 Asn Val Thr Ile Val Ala Ala Thr Pro Phe Leu Phe Val Val Ile Gly 465 470 475 480 Leu Met Phe Ala <210> 453 <211> 945 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> <223> FRXA01591 <400> 453 gcc tca ctt aac tgg tca gtc atc gtt cca gcc cta gtc att gtc cta 48 Ala Ser Leu Asn Trp Ser Val Ile Val Pro Ala Leu Val Ile Val Leu 1 5 10 gcg aca gtg gtg tgg ggt atc gga ttc aaa gat agc ttt acc acc ttt 96 Ala Thr Val Val Trp Gly Ile Gly Phe Lys Asp Ser Phe Thr Thr Phe 25 gct agt tct gcg ttg tca gca gta gtt gac aat Ctc ggc tgg gcc ttc 144 Ala Ser Ser Ala Leu Ser Ala Val Val Asp Asn Leu Gly Trp Ala Phe 40 att ttg ttt ggc aca gtc ttt gta ttt ttt atc gtt gtt atc gcc gct 192 Ile Leu Phe Gly Thr Val Phe Val Phe Phe Ile Val Val Ile Ala Ala 55 agt aaa ttc ggc acg att cgc tta ggc cgc att gat gaa gca cca gag 240 Ser Lys Phe Gly Thr Ile Arg Leu Gly Arg Ile Asp Glu Ala Pro Glu 70 75 ttt cgc acg gtg tca tgg att tcc atg atg ttt gct gca ggt atg ggt 288 Phe Arg Thr Val Ser Trp Ile Ser Met Met Phe Ala Ala Gly Met Gly 90 att ggt ttg atg ttc tac gga acc aca gaa cct tta acc ttc tac cgc 336 Ile Gly Leu Met Phe Tyr Gly Thr Thr Giu Pro Leu Thr Phe Tyr Arg 100 105 110 aat ggt gta cct gga cat gat gaa cac aat gtt ggc gtt gct atg tcc 384 Asn Gly Val Pro Gly His Asp Glu His Asn Val Gly Val Ala Met Ser 115 120 125 acg aca atq ttc cac tgg acc ttg cat cca tgg gct atc tac gca att 432 Thr Thr Met Phe His Trp Thr Leu His Pro Trp Ala Ile Tyr Ala Ile 130 135 140 gtg ggc cta gcc att qcc tat tcg acc ttc cga gtg ggc cgt aaa cag 480 Val Gly Leu Ala Ile Ala Tyr Ser Thr Phe Arg Val Gly Arg Lys Gin 145 150 155 160 WO 01/00805 Ctt cta agc tct gca ttc gtg.cca Leu Leu Ser Ser Ala Phe Val Pro 165 gga tgg ttg ggc aag ctc atc gac Giy Trp Leu Gly Lys Leu Ile Asp 180 ttc ggc acg gca tgt tcc ctt ggc Phe Gly Thr Ala Cys Ser Leu GlyI 195 200 ggc ctg tcc gca gca aac atc att Gly Leu Ser Ala Ala Asn Ile IleC 210 215 gtt ggc att gtt tct gtt ttg acc Val Gly Ile Val Ser Val Leu Thr 225 230 tct ggt gtg ggc aag gga atc cagt Ser Gly Val Gly Lys Gly Ile Gin 245 ctg gca gct ctq.ctc gcg att ttc Leu Ala Ala Leu Leu Ala Ile Phe 260 tcg att ttg aac ctg ctg cca ggtt Ser Ile Leu Asn Leu Leu Pro Gly 275 280 ttc ttt caa atg gta ggc cgc act Phe Phe Gin Met Vai Gly Arg Thr 290 295 ggt gag tgg ctt ggt ggc tgc acc Gly Giu Trp Leu Gly Giy Cys Thr 305 310 <210> 454 <211> 315 <212> PRT <213> Corynebacterium giutamicum <400> 454 Ala Ser Leu Asn Trp Ser Val Ile 1 5 Ala Thr Val Val Trp Gly Ile Gly 1 Ala Ser Ser Ala Leu Ser Ala Val 40 Ile Leu Phe Gly Thr Val Phe Val 1 55 Ser Lys Phe Gly Thr Ile Arg Leu 70 ctc att gqt qaa aaa Leu Ile Gly Giu Lys 170 Itc Ile 185 .,eu ;aa :tg .,eu :ac ryr ~tg /a 1 ~65 :ct 3e r

]CC

kl a ctg Leu gg t Gly gat Asp gca Ala ctc Leu 250 ttc Phe att Ile atg Met att Ile ctg Le u agc Ser 220 atc Ile aa c As n gtc Val1 aac As n gcc Ala 300 atc Ile cag Gin 205 ga t Asp ttc Phe gcc Ala gga Gi y tac T yr 285 gac Asp PCTIBOOIOO926 ggt gca gaa 528 Gly Ala Glu 175 gcc acc gta 576 Ala Thr Val 190 att qqt gca 624 Ile Gly Ala tgg acc atc 672 Trp Thr Ile tcc gct att 720 Ser Ala Ile 240 aac atg gtt 768 Asn Met Val 255 cca acc gtg 816 Pro Thr Val 270 ctg tcc aac 864 Leu Ser Asn ggc aca cca 912 Gly Thr Pro atc ttc tac Ile Phe Tyr 945 Leu Val Ile Val Leu Ser Phe Thr Thr Phe Leu Gly Trp Ala Phe Val Val Ile Ala Ala Asp Giu Ala Pro Glu WO 01/00805 WO 0100805PCTIBOOIOO926 Phe Arg Thr Val Ser Trp Ile Ser Met Met Phe Ala Ala Gly Met Gly 90 Ile Gly Leu Met Phe Tyr Gly Thr Thr Glu Pro Leu Thr Phe Tyr Arg 100 105 110 Asn Gly Val Pro Gly His Asp Glu His Asn Val Gly Val Ala Met Ser 115 120 125 Thr Thr Met Phe His Trp Thr Leu His Pro Trp Ala Ile Tyr Ala Ile 130 135 140 Val Gly Leu Ala Ile Ala Tyr Ser Thr Phe Arg Val Gly Arg Lys Gin 145 150 155 160 Leu Leu Ser Ser Ala Phe Val Pro Leu Ile Gly Glu Lys Gly Ala Glu 165 170 175 Gly Trp Leu Gly Lys Leu Ile Asp Ile Leu Ala Ile Ile Ala Thr Val 180 185 190 Phe Gly Thr Ala Cys Ser Leu Gly Leu Gly Ala Leu Gln Ile Gly Ala 195 200 205 Gly Leu Ser Ala Ala Asn Ile Ile Glu Asp Pro Ser Asp Trp Thr Ile 210 215 220 Val Giy Ile Val Ser Val Leu Thr Leu Ala Phe Ile Phe Ser Ala Ile 225 230 235 240 Ser Gly Val Gly Lys Giy Ile GIn Tyr Leu Ser Asn Ala Asn Met Vai 245 250 255 Leu Ala Ala Leu Leu Ala Ile Phe Val Phe Val Val Gly Pro Thr Val 260 265 270 Ser Ile Leu Asn Leu Leu Pro Gly Ser Ile Val Asn Tyr Leu Ser Asn 275 280 285 Phe Phe Gin Met Vai Giy Arg Thr Ala Met Ser Ala Asp Gly Thr Pro 290 295 300 Gly Giu Trp Leu Gly Gly Cys Thr Ile Phe Tyr 305 310 315 (210> 455 <211> 292 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (292) <223> RXN00201 <400> 455 acgtcgcgga cttcaagtag tcggcgatga aaaagtccgt ttactaaacc ccgatctgtg ttacgccatc gcgcggctcg ggcacaccga tacctgggca gtg gca gat tgc gga 115 WO 01/00805 tta ccc atc cca gaa cac gta gag Leu Pro Ile Pro Giu His Val Giu ggg atc ccc acc ttt gaa caa gta Gly Ile Pro Thr Phe Glu Gin Val gtt gtg gaa ggc gcg gtg att gcc Val Vai Glu Gly Ala Val Ile Ala 45 gaa atg gtg gat acg gat gtg gaa Giu Met Val Asp Thr Asp Vai Glu <210> 456 <211> 64 <212> PRT <213> Corynebacterium giutamicun <400> 456 Val Ala Asp Cys Gly Leu Pro Ilie 1 5 Leu Ala Leu Val Phe Gly Ile Pro Leu Lys Pro Giu Val Val Val Glu 40 Pro Gin Arg Ilie Arg Glu Met Val 55 atc Ile ctg Leu 30 qaa Giu gta Val1 ga t Asp gct Ala gca Al a gcg Ala Val1 1 t tg Le u ctc Le u ccc Pro Ala Asp Cys gca ctc gtg Ala Leu Vai aag ccg gaa Lys Pro Glu caa cgt atc Gin Arg Ile PCTAiBOO/00926 Gily ttt 163 Ph e gta 211 ValI cgc 259 Arg Pro Giu His Vai Gu Ile Ile Asp 10 Thr Phe Giu Gin Val Leu Asn Ala 25 Gly Ala Val Ile Ala Glu Gly Ala Asp Thr Asp Val Glu Val Cys Ala <210> 457 <211> 286 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(286) <223> FRXA00201 <400> 457 acgtcgcgga cttcaagtag tcggcgatga aaaagtccgt ttactaaacc ccgatctgtg ttacgccatc gcgcggctcg ggcacaccga tacctgggca gtg gca gat tgc gga 115 Val Ala Asp Cys Gly 1. tta ccc atc cca gaa cac gta gag atc atc gat ttg gca ctc gtg ttt 163 Leu Pro Ile Pro Giu His Val Gu Ile Ile Asp Leu Ala Leu Val Phe WO 01/00805 PCT/IBOO/00926 15 ggg atc ccc acc ttt gaa caa gta ctg aat gct ctc aag ccg gaa gta Gly Ile Pro Thr Phe Giu Gin Val Leu Asn Ala Leu Lys Pro Giu Val 30 gtt gtg gaa ggc gcg gtg att 9CC gaa ggg gca ccc caa cgt atc c9c Val Val Giu Gly Ala Val Ile Ala Giu Gly Ala Pro Gin Arg Ile Arg 45 gaa atg gtg gat acg gat gtg gaa gtt Giu Met Val Asp Thr Asp Val Giu Val <210> 458 <211> 62 <212> PRT <213> Corynebacterium giutamicum <400> 458 Val Ala Asp Cys Giy Leu Pro Ile Pro Giu His Vai Giu Ile Ile Asp 1 5 10 Leu Ala Leu Val Phe Gly Ile Pro Thr Phe Giu Gin Vai Leu Asn Ala 25 Leu Lys Pro Giu Vai Vai Val Giu Gly Aia Val Ile Ala Giu Gly Ala 40 Pro Gin Arg Ile Arg Giu Met Val Asp Thr Asp Val Giu Val 55 <210> 459 <211> 849 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (826) <223> RXA01221 <400> 459 tccagggtgc gatcctggtt CtCgtgatCg tgttgctgcc gggcggaatt gcttcaatl aactttccgc gctcaaaaat aaggccagga aggccacctc atg agc ctt aaa atc ta acc aac ctc aaa gtc gct ttc ggg tcg ttc atc Thr Asn Leu Lys Val Ala Phe Gly Ser Phe Ile 15 agt ttt cag gtg ctg ccc ggt cac gtc cac ttc Ser Phe Gin Val Leu Pro Gly His Val His Phe 30 ggt gca ggt aaa acc acc tgc att gac gcg atc Gly Aia Gly Lys Thr Thr Cys Ile Asp Ala Ile Met Ser Leu Lys Ile 1 gcc gtg aat gag att Ala Val Asn Giu Ile ctc atc ggt 9CC aac Leu Ile Gly Ala Asn agc gga ttg gcg ccg Ser Gly Leu Ala Pro WO 01/00805 ggg cag gga Gly Gin Gly gtg cac cgc Val His Arg agc gtg ttt Ser Val Phe ggg att cat Gly Ilie His gat ccc cga Asp Pro Arg 120 gtg aat gct Val Asn Ala 135 gaa att gca Glu Ile Ala 150 gag ccg gtg Giu Pro Val ctt ttg cag Leu Leu Gin gat atg gag Asp Met Giu 200 cgc ggc acg Arg Gly Thr 215 ccg gat gtg Pro Asp Val 230 PCTJ~iBOO/00926 tea gtg cag ttg gat ggc act Ser Val Gin L~ egg Arg 75 t tg Leu ttg Le u ca c His gga Gly ctg Leu 155 atg Met g cg Ala cgt Arg tgt Cys a t

S

A

g

A

a

T

I

g

V

a

S

e

A

e

A

g

G

2 ~eu Asp 60 tg ggt let Gly ct gtg er Val 'gg gcg .rg Ala cc ctg la Leu 125 cc ttg hr Leu 40 tg cag al. Gin gt gag er Giu gg gga .rg Gly gt ttt .rg Phe 205 ag ggg lu Gly 20 Thr ggg Gly cag Gin ctc Le u gt c Val cat His gcg Al a gag Giu 175 gtg ValI act Th r gtc ValI gag Glu ega Arg aat Asn ggg Gly a cg Thr ggg Giy cag Gin 160 cgt Arg gtg Val1 cgc Arg gat Asp att ctg gga Ile Leu Gly aeg ttt cag Thr Phe Gin etg gat att Leu Asp Ile gtg egt eat Vai Arg His 115 ggt ctt get Gly Leu Ala 130 cag aaa cag Gin Lys Gin 145 gtg etc atg Vai Leu Met gte gca aeg Val Ala Thr ttg gtg gtg Leu Val Val 195 gte act gtg Val Thr Val 210 gag att eag Glu Ile Gin 225 ace Thr ace Thr geg Ala 100 egg Arg gat Asp tgg Trp etg Leu ggt Gly 180 gag Glu atg Met g eg Al a 307 355 403 451 499 547 595 643 691 739 787 836 849 tee att tat tta Ser Ile Tyr Leu 235 aeg gca ggg aag tgagttagtc Thr Ala Gly Lys 240 atgttggaaa tea <210> 460 <211> 242 <212> PRT <213> Corynebacterium glutamicum <400> 460 Met Ser Leu Lys Ile Thr Asn Leu Lys Vai Ala Phe Gly Ser Phe Ile 1 5 10 WO 01/00805 Ala Val Asn Leu Ile Gly Ser Giy Leu Ile Leu Gly Thr Phe Gin Leu Asp Ile Val Arg His 115 Giy Leu Ala 130 Gin Lys Gin 145 Vai Leu Met Vai Ala Thr Leu Val Val 195 Val Thr Val 210 PCTIBOO/00926 Giu Ile Ser Phe Gin Vai Leu Pro Gly His Val His Phe 25 Ala Ala Thr Th r Al a 100 Arg Asp Trp Le u Gi y 180 Gi u Met Asn Pro Pro Ala Cys Ile Le u Leu Asp 165 Gi u His Asn Gly Gi y Val1 Se r Gi y Asp ValI Giu 150 Giu Le u Asp Arg Ala Gin His Val1 Ile Pro Asn 135 Ile Pro Le u Met Gly 215 Gly 40 Gly Arg Phe His, Arg 120 Al a Ala Val1 Gin Giu 200 Thr Ile Asp Asp Gly Gly Val Val Leu Ala Leu 110 Leu Giu 125 Leu Ser Gin Asp Giu Glu Gly Arg 190 Phe Ala 205 Gly. Ser Ala Thr Gly Gin Leu Val1 His Ala Glu 175 Val1 Th r Val1 Giu 225 Gly Gin Ala Asn Asp Val Gin Ser Tyr Leu Gly Thr <210> 461 <21 1> 822 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (799) <223> RXA01222 <400> 461 aatcgcggca cgatcttgtg tgaggggtcg gtcgatgaga ttcaggcgaa tccggatgtg cagtccattt atttaggtac ggcaqggaag tgagttagtc aig ttg gaa atc act 115 WO 01/00805 aat ttg tgt gca ggt tat qqc Asn Leu Cys Ala atc Ile gct Ala act Thr a cg Thr cag Gin ga t Asp cgc Arg t cg Se r g cg Ala 150 t cg Se r ggC Gly gct Ala gga Gly a cg Th r aaa Ly s gg a Gly gag Glu ttt Phe cag Gin ccg Pro 120 ggt Gly aag Lys gtt Val1 agc Ser aca Thr 200 ggC Gly agc Ser tcc Ser gag Giu cg a Arg acg Th r gga Gly 105 gcg Al a cag Gin ctt Leu gc t Al a att Ile 185 agc Se r gc t Aila Gi y agc Ser acc Th r gtc Vai gta Val cag Gin cg t Arg ct g Le u cgt Arg ttg Le u ga a Giu 170 gtc Vai tac Tyr ga a Glu Tyr Giy cgc acg Arg Thr aac Asn ttg Le u aaa Lys aag Lys 75 ct t Le u gt g Vai acc Thr ca g Gin ct t Le u 155 at t Ilie ctg Leu gcc Al a acc Thr ggc atc Gly Ile ctg cga Leu Arg 45 ctt ttc Leu Phe cgc gga Arg Gly agt tgc Ser Cys ggc aag Giy Lys cag gtg Gin Val 125 cag ctt Gin Leu 140 ctc gac Leu Asp cag cag Gin Gin gtg gag Vai Glu att gtg Ile Val 205 acc gca Thr Ala ctg Leu 30 acc Thr gg C Gly a tg Met atg Met gca Ala 110 ct g Le u gcc Al a gaa Giu acc Th r caa Gin 190 gcq Ala gag Glu cag gta Gin Val 15 tcg atc Ser Ile gcg gtg Ala Vai cag gat Gin Asp gct tat Ala Tyr 80 gaa aat Giu Asn 95 cgc atc Arg Ile gac cgc Asp Arg atc gcc Ile Ala ccc acc Pro Thr 160 atc atc Ile Ile 175 aac att Asn Ile cgt ggt Arg Giy aag cag Lys Gin Met Leu Giu 1 ctt cat tct Leu His Ser ctc ggc cac Leu Giy His ggg ttg att Gly Leu Ilie gtc acc tcg Vai Thr Ser gtg ccg cag Val Pro Gin ttg cag gtg Leu Gin Val gcg gag gcg Ala Giu Aia 115 caa gcc ggc Gin Aia Gly 130 cgc gcg ctg Arg Ala Leu 145 gag ggt att Glu Gly Ile gat ttg gct Asp Leu Ala ggt ttt gca Gly Phe Ala 195 cag gtc gtg Gin Vai Val 210 act aaa gtg Thr Lys Val 225 Ile ctt Le u aat Asn aag Lys t tg Le u ggc Gly gta Val1 100 ct t Leu Ctg Le u atc Ile cag Gin aag Lys 180 ttg Leu gaa Giu cgg Arg PCTIBOO/00926 Thr tca 163 Ser ggc 211 Gly ccg 259 Pro tcc 307 Se r cag 355 Gin gcg 403 Ala gat 451 Asp ttg 499 Le u acg 547 Th r cct 595 Pro 165 gac 643 Asp gat 691 Asp tcg 739 Ser gaa 787 Glu tct cta gca atc Ser Leu Ala Ilie tagcggctgt ggatagcgtt ttg WO 01/00805 WO 0100805PCT/tB00100926 230 <210> 462 <211> 233 <212> PRT <213> Corynebacterium giutamicun <400> 462 Met Leu Glu Ile Thr Asn Leu CysJ Leu His S, Leu Gly H.

Gly Leu I.

Vai Thr Si Val Pro G.

Leu Gin V.

Ala Glu A.

1: Gin Ala G.

130 Arg Aia Li 145 Giu Gly I.

Asp Leu A.

Giy Phe A: 1, Gin Val V, 210 Thr Lys V.

225 <210> 463 Leu As n Ly s Le u Giy Vali 100 Le u Le u Ile Gin Ly s 180 Le u G.1u Arg Se r Gly Pro Se r Gin Aia Asp Leu Th r Pro 165 Asp Asp Se r Glu Ile Ala Th r Thr 70 Gin Asp Arg Ser Ala 150 Ser Gly Ala Gly Se r 230 Ser Gly Ser 55 His Ser Leu Phe Gly 135 Pro Val1 Met Ala Gin 215 Le u Th r Lys Giy Giu Ph e Gin Pro 120 Gly Lys Val1 Se r Thr 200 Gly Ala Gly Giy Le u Le u Arg Se r Giy Gin Gin 140 Leu Gin Val1 Ile Th r 220 Arg Ile Arg Phe Gly Cys Lys Val1 125 Le u Asp Gin G iu Val1 205 Ala Th r Le u Th r Gly Met Met Ala 110 Le u Ala Giu Th r Gin 190 Al a Glu Gin Ser Ala Gin Ala Giu Arg Asp Ile Pro Ile 175 Asn Arg Lys Val1 Ile Val1 Asp T yr Asn Ile Arg Ala Thr 160 Ile Ile Gi y Gin <211> 1005 <212> DNA <213> Corynebacterium glutarnicum <220> WO 01/00805 <221> CDS <222> (101)..(982) <223> RXA01219 <400> 463 caccatttgg gaaaccgatt ccccagttga tccggaccca PCT/IBOO/00926 tacttgtctt cctatgactg ggccaagacc accgctgcga cttcctaaga gataaaaatc atg gac att tta ctc Met Asp Ile Leu Leu caq Gin gtg Val1 cac His Ct 9 Le u gcc Ala ct g Le u 99 t Gly

CCC

Pro gtt Val 135 gcc Ala tg Trp acc Th r ctc Leu gga Gly gga Gly gt c ValI ttt Phe aag Lys gtc Val1 gca Ala 120 cta Le u atc Ile 99C Gly gca Ala gcc Ala t ca Se r ttc Phe ggt G ly atc Ile ctt Leu ttg Le u gat Asp gtc Val1 tca Ser cgC Arg 170 att Ile ggg Gly ctq Le u atc Ile tct Ser gg t Gly tat Tyr atc Ile gtc Val1 t tq Leu gtg Val1 155 atc Ile gat Asp ctt Leu acc Thr atg Met gcc Al a 60 ggg Gly cac His ctt Le u agg Arg gtg Val1 140 act Th r cgc Arg acc Thr tca gtt gga Ser Val Gly 15 ttt gga cag Phe Gly Gin gtC ggc gca Val Gly Ala 45 ggt tta tcc Gly Leu Ser ctt ttc gga Leu Phe Gly agg cca cta Arg Pro Leu 95 cag cag ctg Gin Gin Leu 110 gca CCg gaa Ala Pro Giu 125 CCg aCC gCg Pro Thr Ala gca Cta gct Ala Leu Ala gcc gtg gtt Ala Val Val 175 cga gCt act Arg Ala Thr 190 atc 9CC ggg Ile Ala Gly 1 gtc Val1 Gly acc Thr Ctg Le u ctc Le u a eq Thr cga Arg ctc Le u ct a Le u 145 ttc Phe aac As n cg a Arg gct Ala Ctt Leu gt t Val1 gca Ala atc Ile ctc Le u Ctg Leu aac As n cgC Arg 130 t tC Phe cta Leu C9C Arg atg Met atc Ile Cta Leu att Ile tat Tyr a gc Se r ga a Giu ctg Leu att Ile 115 gga Gly atc Ile aat As n gaC Asp a Cg Thr 195 aca Thr t tg Le u aat As n gtg Val1 att Ile caa Gin gCC Ala 100 ttC Phe aac Asn ctg Le u CgC Arg ctc Le u 180 ttc Phe ttg Le u gt C Val1 atg Met gtq Val CCg Pro ttc Phe aca Thr gga Gi y gt c Val gcg Ala act Thr 165 g cg Ala ttt Phe att Ile 115 163 211 259 307 355 403 451 499 547 595 643 691 739 gtg ggc tCC ggt ctt gcc gga Val Gly Ser Gly Leu Ala Gly WO 01/00805 200 205 ggjc gcg acc ggc ccc acc atc ggt cag Gly Ala Thr Gly Pro Thr Ile Giy Gin 215 220 ctt gtt gtt gcc gcc ggt qgc atc ggc Leu Val Val Ala Ala Giy Giy Ile Giy 230 235 atg gct ttc gtg ctg gga att act caa Met Ala Phe Val Leu Gly Ile Thr Gin 250 ggt gcg agc atc gcg aag ttc atc gta Gly Ala Ser Ile Ala Lys Phe Ile Val 265 270 ctg cag ttt agg cct caa gga ctc ttc Leu Gin Phe Arq Pro Gin Gly Leu Phe 280 285 qta tgagcactca actcaagctg aag Val <210> 464 <211> 294 <212> PRT <213> Corynebacterium giutamicum <400> 464 Met Asp Ile Leu Leu Asn Gin Leu Val PCTIBOO/00926 210 gtg Val1 ggC Gly ga a Giu gtt Val act Thr 290 ttc Phe atc Ile 245 aca Thr ttc Ph e ctc Le u 1005 1 Vali Gly Thr Leu Leu Thr Arg Le u Le u Val1 Al a Ile Le u Le u Asn Arg 130 Le u Ile Tyr Ser Gi u Le u Ile 115 Gly Leu Asn Val1 Ile Gin Ala 100 Phe Asn Val1 His Leu Ala Leu Gly Pro Val1 Gly Gly Val1 Ph e Lys Val Al a 120 Le u Ala 10 Se r Phe Gly Ile- Le u Le u Asp Val1 Ser Val1 Gly Gly Le u Ph e Pro Gin 110 Pro Th r Le u Gly Gin Al a Ser Gly Le u Leu Glu Ala Ala Ser Met Tyr Le u Val1 Asp Ala Ph e Arg Val1 Leu Phe Ile Leu Ala Leu Ala Ile Ala WO 01/00805 145 150 155 Phe Leu Asn Arg Thr Ala Trp Gly Arg Arg Ile 165 170 Asn Arg Asp Leu Ala Giu Thr Ala Gly Ile Asp 180 185 Arg Met Thr Phe Phe Val Gly Ser Gly Leu Ala 195 200 Ala Ile Thr Leu Ile Gly Ala Thr Gly Pro Thr 210 215 Ile Val Asp Ala Phe Leu Val Val Ala Ala Gly 225 230 235 Lys Gly Ala Val Ile Met Ala Phe Val Leu Gly 245 250 Val Giu Tyr Thr Thr Gly Ala Ser Ile Ala Lys 260 265 Ala Val Val Ala Phe Leu Gin Phe Arq Pro Gin 275 280 Gin Thr Arg Ser Leu Val 290 <210> 465 <211> 1200 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (1177) <223> RXA01220 <400> 465 caggtgcgag catcgcgaag ttcatcgtac tcatcgctgt ggcctcaagg actcttccaa acccaaacta ggagcctcgt Arg Thr Gly Ile 220 Gly Ile Phe Gly Val1 Al a 190 Ala Gin Gly Gin Val1 270 P he PCTIIBOO/00926 160 Leu Asp Val Tyr Val1 240 Phe Ile Thr aaq Lys gta ValI gc t Al a a cg Thr gca Ala ct g Le u tgc Cys ttg Le u act Thr gc9 Ala ttt Phe 9 c9 Ala a cg Thr tt g Leu tta Le u atc Ile tgttgccttc ctgcagttta atg agc act caa ctc Met Ser Thr Gin Leu 1 ccg aaa ctt agc gtc Pro Lys Leu Ser Val ggc ctg gcc gcg ctc Gly Leu Ala Ala Leu tcc aca ttc cag ctg Ser Thr Phe Gin Leu gtc gca. gtc ggc atc Val Ala Val Gly Ile 115 163 211 259 307 WO 01/00805 PCT[IBOO/00926 60 ggg ctg gcg tgg ggc aga ggc ggc atg ctc acg ctg ggg caa ggc gtg 355 Gly Leu Ala Trp Gly Arg Gly Gly Met Leu Thr Leu Gly Gin Gly Val 75 80 ttc ttt gga atc ggc gcg tac atc atg gcc atg cac atg ctg tac agc 403 Phe Phe Gly Ile Gly Ala Tyr Ile Met Ala Met His Met Leu Tyr Ser 95 100 gat tcg cag att ttt ggg acc aca gtt ccg caa tgg tgg tcc att ttt 451 Asp Ser Gin Ile Phe Gly Thr Thr Val Pro Gin Trp Trp Ser Ile Phe 105 110 115 gcc aac ccg qca qtc gca ctc atc gca gtt gtg gcg ctt ccc ggc atc 499 Ala Asn Pro Ala Val Ala Leu Ile Ala Val Val Ala Leu Pro Gly Ile 120 125 130 gtg gct ttt gtg ctc ggc ttc tcc att ttc aaa cga cgc atc aaa qgc 547 Val Ala Phe Val Leu Gly Phe Ser Ile Phe Lys Arg Arg Ile Lys Giy 135 140 145 gcc tac ttt gcc atc gtg aac caa gcg ctc gcc gca gct gtt gtg gtg 595 Ala Tyr Phe Ala Ile Val Asn Gin Ala Leu Ala Ala Ala Val Val Vai 150 155 160 165 ttg ctg gtc gga caa caa gat tcc ctt gga ggt tcc aat ggt ctt tcc 643 Leu Leu Val Giy Gin Gin Asp Ser Leu Gly Gly Ser Asn Gly Leu Ser 170 175 180 gga ttt cga tcg ttc atg ggt ttt gcc gtc tac gac ccc atc aac cgc 691 Giy Phe Arg Ser Phe Met Giy Phe Ala Vai Tyr Asp Pro Ile Asn Arg 185 190 195 atc atg ttt tac ttc acc gca gtg gga gtt ctc ttg gct ttg gtg gct 739 Ile Met Phe Tyr Phe Thr Ala Vai Gly Val Leu Leu Ala Leu Vai Ala 200 205 210 atc tca tat tgg ctc atg cgc agc cgc tat gga gaa ctg ctc gtg gcc 787 Ile Ser Tyr Trp Leu Met Arg Ser Arg Tyr Gly Giu Leu Leu Val Ala 215 220 225 acc aga gat gca gaa gaa cgc gtc cqa ttc ctc gga tat gat ccc gca 835 Thr Arg Asp Aia Glu Glu Arg Val Arg Phe Leu Giy Tyr Asp Pro Ala 230 235 240 245 ttg atc aaa acc gcc gca tat gtc att gct gcg atg att gcc gga atc 883 Leu Ile Lys Thr Ala Ala Tyr Val Ile Ala Ala Met Ile Ala Giy Ile 250 255 260 gcc gga gcg ctg ttc gtg ccg atc qtg ggc atc att tca ccc gca gaa 931 Ala Gly Ala Leu Phe Val Pro Ile Val Gly Ile Ile Ser Pro Ala Glu 265 270 275 atc ggc gtg gtg cca tca atc gtg ttc gtg atc gcc gtc gcc gct ggt 979 Ile Gly Val Val Pro Ser Ile Val Phe Val Ile Ala Val Ala Ala Gly 280 285 290 ggc agg gca tcc cta ttc ggt ccc gta gtt ggc gcg ctg gtg ctg ggc 1027 Gly Arg Ala Ser Leu Phe Gly Pro Val Val Gly Ala Leu Val Leu Gly 295 300 305 WO 01/00805 WO 0100805PCTIIBOO/00926 t gg Trp 310 ttc Phe att Ile acc Thr gtg Val1 cag Gin gct Al a t ca Se r gaa tcc aca ctt gct caa act ttc ccc agc atg tgg tcc tat Giu Ser Thr Leu Ala Gin Thr Phe Pro Ser Met Trp Ser Tyr 315 320 325 99t gcg atc ctg gtt ctc gtg atc gtg ttg ctg ccg ggc gga Giy Ala Ile Leu Val Leu Val Ile Val Leu Leu Pro Gly Gly 330 335 340 tca att aaa ctt tcc gcg ctc aaa aat aag gcc agg aag gcc Ser Ile Lys Leu Ser Ala Leu Lys Asn Lys Ala Arg Lys Ala 345 350 355 tgagccttaa aatcaccaac ctc 1075 1123 1171 1200 <210> 466 <211> 359 <212> PRT <213> Corynebacterium giutamicum <400> 466 Met Ser Thr Gin Leu Lys Leu Lys Lys Leu Thr Aia Giy Met T rp Leu 130 Arg Ala As n Le u Ala Phe Val1 Gin Le u Ser 115 Pro Ile Val1 Giy Se r Ala Gin Giy Giy Tyr 100 Ile Gi y Ly s Val1 Le u Val1 Al a Thr Gly Ph e Asp Al a Val1 Ala 150 Leu Gly Asn Ala Le u 55 Le u Phe Ser As n Aia 135 Tyr Le u Phe Lys Pro Pro Thr 25 Leu Leu Ser Arq Trp Gly Ile Gly Ile Phe 105 Ala Val Val Leu Ala Ile Gly Gin 170 Ser Phe 185 Tyr Phe Ly s Arg Al a Val1 Gly Tyr Th r Leu Phe 140 Asn Asp Giy Ala L ys Ala Le u Tyr Met Met ValI 110 Ala Ile Al a Le u Ala 190 Gly Thr Ala Phe Ala Le u Ala Pro Val1 Phe Le u Gly 175 Val1 Val1 Thr Le u Le u Ile Th r Met Gin Val1 Lys Al a 160 Gly T yr Leu 180 Asp Pro Ile Asn Arg Ile Met Phe WO 01/00805 195 Leu Ala Leu 210 Giu Leu Leu 225 Gly Tyr Asp Met Ile Ala Ile Ser Pro 275 Ala Val Ala 290 Ala Leu Val 305 Ser Met Trp Leu Leu Pro Lys Ala Arg 355 PCT/IB00100926 Val1 Val1 Pro Gly 260 Ala Ala Le u Se r Gly 340 Lys Al a Al a Al a 245 Ile Gi u Gly Gly Tyr 325 Gly Ala Trp Ala Thr Leu 265 Val1 Ser Se r Ala Ile 345 Le u Glu Al a 250 Phe Pro Le u Thr Ile 330 Lys Met Glu 235 Ala Val1 Se r Phe Le u 315 Le u Le u 205 Se r Val1 Val1 Ile Val1 285 Pro Gin Leu Ala Arg Arg Ile Val1 270 Phe Val1 Thr ValI Le u 350 <210> 467 <211> 876 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(853) <223> RXA00091 <400> 467 tcatgaacca cgtgttccgc gcacaaggcg tcgtgtccat gtaccgtctt cctcatcgtc atcctcagaa agggcagact aat gtc Asn Val ctt gag Leu Glu qca ggc Ala Gly cgc aag gaa tac tcc Arg Lys Glu Tyr Ser atc cca gcc ggc ggc Ile Pro Ala Gly Gly aag tca aca ctg ctc Lys Ser Thr Leu Leu gtt gcc Val Ala 15 gcg tig Ala Leu atc ggt Ile Gly cattatigag atggtcgqcg gtg att acg tta act Val Ile Thr Leu Thr 1 atc qgc ccc gtc aac Ile Gly Pro Val Asn gtc ggc cca aac qqt Val Gly Pro Asn Gly cga ctc ctc ggc atc Arg Leu Leu Gly Ile 115 163 211 259 WO 01/00805 WO 0100805PCTfIBOOIOO926 ga t Asp tcc Se r ttt Ph e cca Pro cg c Arg gac Asp ctg Leu 150 ctt Leu gca Ala ttc Phe atc Ile tca Se r 230 gaa Glu aaa L ys gtt Val1 tat Tyr tac Tyr cag Gin 135 tgc C ys gat Asp g cc Ala gcc Ala gaa Giu 215 ga a Giu gg C Gi y ga t Asp acc Th r agc Ser atc Ilie 120 ctt Le u caa Gin atc Ile caa Gin 9g Ala 200 a aa Lys atc Ile atc Ile g cc Ala ctg Leu ggC Gi y ttc Phe ggc Gly a cg Th r cac His 170 ggC Gly tac Tyr gga Gly aat Asn acc Th r aag Lys 75 acc Thr cgg Arg ttc Ph e gg C Gly ga c Asp 155 tcg Se r cg c Arg gcc Ala aca Th r aca Thr 235 g ta Val1 60 atc Ile gtg Val1 ctg Leu aat As n ca g Gin 140 tac Tyr gtg Val1 acc Thr gat Asp cct Pro 220 gag Giu gcc Ala atc Ile cgc Arg acg Thr ctc Leu 125 cgc Arg gtg Val1 gaa Glu atc Ile tac Tyr 205 gaa Giu atc tcc Ser tcc Se r ca g Gin gaa Giu 110 acc Th r ca g Gin ctt Leu atg Met atc Ile 190 atc Ile cag Gin gaa tac Tyr atc Ile ctc Le u 95 gaa Giu gaa Giu cg c Arg ctc Le u atg Met 175 gt g Val1 gtg ValI atc Ile gtt ga t Asp ct g Le u 80 gta Val1 gac Asp ctc Le u gcc Ala ga c Asp 160 aaa Lys gtt Val1 gcc Ala atg Met att gtc Val1 cg C Arg ggt Gly gag Glu ga a Glu tat Tyr 145 gaa Giu cac His ctt Le u gta Val1 aaa Lys 225 gaa tca Se r gaa Glu gga Giy a tc Ile 115 cgc Arg gcc Ala ct c Leu gag Glu gac Asp 195 cac His gag Giu cca acc Th r aac Asn cq c Arg 100 atc Ile tac Tyr atg Met aac As n aat Asn 180 atc Ile gga Gi y atc Ile cac g ca Ala cac His ttc Phe t cc Ser ctc Le u g tg Vai aat Asn 165 gct Ala aac Asn a tg Met ctt Leu qg C Gly 307 355 403 451 499 547 595 643 691 739 787 835 Ile Giu Val Ilie Glu Giy Pro His 240 aag att gct Lys Ile Ala tgc tac cac taaatgaaga gaaataagcc gac Cys Tyr His <210> 468 <211> 251 <212> PRT <213> Corynebacterium giutamicun <400> 468 Val Ile Thr Leu Thr Asn Val Arg Lys Glu Tyr Ser Ser Asp Val Ala WO 01/00805 1 Ile Giy Pr Val Giy Pr 3 Arg Leu Le Val Thr Se Arg Gin Gi Giy Phe Gi Giu Giy II Giu Asp Ar 130 Tyr Vai Al 145 Giu Pro LE His Leu G1 Leu His AE Vai Lys Hi 210 Lys Asn GI 225 Giu Giy Pi PCT/IBOO/00926 5 10 Vai Asn Leu Giu Ile Pro Ala Gly Giy Ile Thr Ala Leu Gi y Ile Al a His Phe Se Le u Val1 Asn 165 Ala Asn Met Leu Ala Asp Se r 70 Phe Pro Arg Asp Le u 150 Leu Al a Phe Ile Se r 230 Gly Gi u 55 Lys Val1 Tyr Tyr Gin 135 Cys Asp Ala Ala Giu 215 Glu Thr Ile Ala Le u 90 Gi y Phe Gly Th r His 170 Gly Tyr Gly Asn Le u Thr Ly s 75 Th r Arg Phe Gly Asp 155 Se r Arg Ala Thr Thr 235 Leu Val1 Ile Val Le u Asn Gin 140 Tyr Val1 Thr Asp Pro 220 Gi u Thr Ala Ile Arg Th r Le u 125 Arg Val1 Gi u Ile Tyr 205 Giu Ile Met Ser Ser Gin Giu 110 Thr Gin Le u Met Ile 190 Ile Gin Glu Ile Tyr Ile Leu Glu Gi u Arg Le u Met 175 Val1 Val1 Ile Val1 Gly Asp Le u Val1 Asp Le u Ala Asp 160 Lys Val1 Ala Met Ile 240 His Gly Lys Ile Ala Cys 245 Tyr His 250 <210> 469 <211> 692 <212> DNA <213> Corynebacterium glutamicum <22 0> <221> COS <222> (79)..(669) <223> RXA00228 <400> 469 ccqcatggca tccgccgaaa gaattcccct ccggaggaat ggcacgcctc atgggagaac WO 01/00805 PCTIBOO/00926 gcateggcaa aaaetgacatg cgc gac ctc cgc tee atg atc ggc gte agc 11 Met Arg Asp Leu Arg Ser Met Ile Giy Val Ser 1 5 tee tee gca tta ggc aac cga atc ccc tcc qaa gaa aaa qtc tet gac 159 Ser eta Leu tac Tyr gga Gly gaa Giu etc Leu gaa Glu ect Pro tte Phe 140 etg Leu eag Gin Asn gge Giy ttc Phe gcc Ala 65 etg Le u gaa Giu tac Tyr atc Ile etc Le u 145 atg Met gac Asp Arg t ac Tyr gga Gly ga c Asp gte Val1 eca Pro etc Leu ace Th r 130 etc Le u aca Thr ege Arg Giu eg e Arg ate Ile ace Thr a tg Met gac Asp atg Met gaa Giu 135 ate Ile tee Ser tac Tyr Lys Val Ser Asp tgg ege gaa gac Trp Arg Glu Asp etc gaa caa gte Leu Giu Gin Val etc tee gaa gge Leu Ser Giu Gly aec aac ceg gaa Thr Asn Pro Glu etc gge gga ege Leu Gly Gly Arg 105 gae eca gac gea Asp Pro Asp Ala 120 atc ccc gee gga Ile Pro Ala Gly gta gee caa gge Val Ala Gin Gly 155 aaa gca tte eac Lys Ala Phe His 170 ttt gee ege egt Phe Ala Arg Arg 185 gtg aga ace gec agg agt eat agg get cag Val Arg Thr Ala Arg Ser His Arg Ala Gin 190 195 gee <210> 470 <211> 197 <212> PRT <213> Corynebacterium qiutamiewn <400> 470 taggtttttt ggagttgtgg WO 01/00805 Met Arg Asp 1 Asn Arg Ile Gly Tyr Ala Phe Gly Gin Ala Asp Arg Leu Val Ala Giu Pro Thr Tyr Leu Gly 115 Ile Thr His 130 Leu Leu Asp 145 Met Thr Asn Asp Arg Ile Ser His Arg 195 Leu Pro Ile Ala Thr Arg Ala 100 Glu His Giu Giu Gly 180 Ala Arg 5 Ser Leu Thr Trp Ala Gly Leu Val1 Gi y As n 165 Gi u Gin Met Glu Ar g Ilie 55 Thr Met Asp Met Glu 135 Ilie Se r Tyr Ser Asp Asp Val1 Gly 75 Giu Arg Ala Gly Gly 155 His Arg Se r Leu Tyr Gly Giu Le u Giu Pro Phe 140 Leu Gin Val1 PCTIBOOIOO926 !u Gly r Ala !t Asp .s Leu g Val !u Asp .1 Gly .1 Met .a Met ir Val 160 .n Vai .a Arg <210> 471 <211> 813 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (790) <223> RXA00346 <400> 471 atctatgaga cccctcaaaa caccgagaat ttcctcgatg cattcaccaa qgcagttgat gatctcaccg ctgccactaa ccaggtttag aattatttaa atg ctg ttg act ttc 115 Met Leu Leu Thr Phe 1 aat gat gct gcg gtg gat ccc ctc tgg agg ggc ctg aat tta gag ctc 163 Asn Asp Ala Ala Val Asp Pro Leu Trp Arg Gly Leu Asn Leu Giu Leu 15 WO 01/00805 cga cag ggg Arq Gin Gly tcc acg ctc Ser Thr Leu tcg gtt aaa Ser Val Lys ttc gat gtc Phe Asp Val cat ggc qtg His Gly Val ctt ctt gcc Leu Leu Ala gag ctc tcc Glu Leu Ser 120 gcc acg cgc Ala Thr Arq 135 gac ccc ggc Asp Pro Gly 150 gcc gaa ggc Ala Giu Gly atg ggc ctg Met Gly Leu atc ggc acg Ile Gly Thr 200 tac aac gca gaa Giu atc Ile act Th r ccg Pro gt t.

Val1 cg c Arg 105 Gi y ccg Pro gt c Val1 gtc Val gt a Val1 185 gtt Val ccc ttt Phe gg t Gly ga t Asp ttg Leu tcc Ser g tg Val1 ggg Gly caa Gin gcg Al a ggc Gly 170 gat Asp ggc Gi y gt c ctt Le u a cg Thr gcc Al a cg t Arg 75 aaa Lys ggc Gly cag Gin tta Leu cag Gin 155 gtt Val1 cgc Arg gat Asp a cg gcg Al a att Ile cg g Arg 60 gcc Ala agg Arg gct Al a ca g Gin ttg Leu 140 cg c Arg gt t Val1 att Ile gtc Val1 gtg tta Le u ggc Gly ggt Gly ga t Asp ccc Pro gga Gly 110 ctc Leu gcc Ala gtg Val1 gtc Val1 tac Tyr 190 cag Gin cg c Arg ggc Gly acc Thr tat Tyr a tg Met gcg Al a 95 atc Ile gtc Val gac Asp t cc Ser acc Thr 175 ctc Le u tcc Se r atc Ile ccc Pro cga Arg att Ile gtt Val1 80 aag Lys gcc Ala cgc Arg gaa Giu cta Leu 160 cac His gcc Ala ga a Glu aac Asn aac Asn aaa Lys5 ccg Pro tcg Ser gg t Gly ga t Asp ca a Gin ccc Pro 145 ttt Phe ga t Asp ccc Pro aaa Ly s ga c Asp 225 ggc Gly ctc Le u caa Gin ctg Le u gac Asp cqa Arg gcc Ala 130 ctc Leu gg t Gly gt c Val1 aac Asn ctc Leu 210 aga Arg PCT/IBOO/00926 gga aaa 211 Gly Lys cac ggt 259 His Gly cga att 307 Arg Ile gcg gcg 355 Ala Ala gat aag 403 Asp Lys 100 gtc ggc 451 Val Gly gcc ctt 499 Ala Leu agc ctt 547 Ser Leu ttg aag 595 Leu Lys 165 cca cta 643 Pro Leu 180 cac acc 691 His Thr gaa ctc 739 Giu Leu gtg gtg 787 Val Val Tyr Asn Ala Pro Val Thr Val Ala 215 220 gtt taagtggatc tatccacctg gct Val 230 <210> 472 <211> 230 <212> PRT <213> Corynebacterium giutamicum WO 01/00805 WO 0100805PCTIBOOIOO920 <400> 472 Met Leu Li 1 Leu Asn Li Asn Gly V.

Lys Leu T] Pro Gin G.

Ser Leu Si Gly Asp V.

Asp Arg A:

I

Gin Ala G 130 Pro Leu Li 145 Phe Gly G.

Asp Val A Pro Asn G.

1~ Lys Leu Si 210 Asp Arg I.

225 eu Thr Phe 5 eu Giu Leu al Gly Lys h~r His Gly In Arg Ile er Ala Ala 31 Asp Lys 100 rg Val Gly 15 In Ala Leu eu Ser Leu lu Leu Lys 165 sn Pro Leu 180 ly His Thr 95 er Glu Leu le Val Val Asn Asp Ala Ala Val Asp Pro Leu Trp Arg 10 Gin Thr Val1 55 Asp Gly Le u Leu Thr 135 Pro Glu Gly Gly Asn 215 Gi y Le u 40 Lys Val1 Val1 Al a Ser 120 Ar g Gly Gly Leu Th r 200 Ala Glu Ile Thr Pro Val1 Arg 105 Gl y Pro Val1 Val1 Val1 185 Val1.

Pro Phe Gly Asp Leu Ser 90 Val1 Gly Gin Ala Gly 170 Asp Gi y Val Le u Th r Ala Arg 75 Lys Gly Gin Leu Gin 155 Val1 Arg Asp Thr Ala Ile Arg Ala Arg Ala Gin Leu 140 Arg Val1 Ile Val1 Vali 220 Vali Leu Val1 Arg Gly Se r Gin 125 Le u Th r ValI Leu Met 205 Ala Gly Thr T yr Met Ala Ile Val1 Asp Ser Thr 175 Le u Ser Ile Gi y Pro Ar g Ile ValI Ly s Al a Arg Glu Le u 160 His Ala Gi u Asn <210> 473 <211> 433 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(433) <223> RXA00524 <400> 473 tcctcggcac ccgctacccc gtcggagttg tcaccggcgc attcggcgcc ccattcctta WO 01/00805 tctatitact cattcgttcc aaccgcgcgg gagiaaccct PCTIBOOIOO926 gig acc acc aac cat 115 Val Thr Thr Asn His caa Gin atc Ile gi c Val1 cgc Arg cti Le u ccg Pro 9qc Gi y cta Le u ga t Asp ggc Giy cic Le u cct Pro ca a Gin cg c Arg tcc Ser t cg Ser ccc Pro cti Le u ica Ser icc Se r 9gc Gly gcc Aila Cc Le u aac As n aaa Lys cig Le u ccc Pro cgc Arg 105 ati Ile ga c Asp Giy gcc Aila aaa Ly s cci Pro cac His icc Se r aic Ile aaa Lys ggg Gly gaa Giu gaa Glu caa Gin

C

I

:ig Le u ;Ic la I 30 :ca 3er :a a 31n :ta ,e u iy )g a iy 110 kla .,eu 25 ksn ,ys Le u Pro krg 105 gcg Ala ccc Pro acq Th r gcg Al a gc i Aia aic Ile cic Le u Gi u Se r Gly Pro Pro Th r His tac T yr 99c Giy cig Le u cii Leu cg c Arg gi c Val1 Glu Val1 Cys Se r Gly 75 Gly Pro cgc Arg acc Thr gcc Ala g cc Ala ggg Gly ga c Asp Leu Val Se r Gin Le u Gly Gly 110 <210> 474 <211> Ill <212> PRT <213> Corynebacterium glutamicum <400> 474 Vai Thr Thr Asn His Gin Leu SerJ 1 5 Gly Glu Arg Thr Ile Ile Asp SerI Lys Ile Thr Ser Ile Val Gly Pro Leu Arg Ala Phe Ala Arg Leu LeuI Ile Asp Ala His Pro Leu Pro SerI 70 Mei Leu Gly Leu Leu Pro Gin Ser Vai Ala Asp Leu Val Giy Arg Gly 100 Tyr Giy Leu Leu Arg Val WO 01/00805 <210> 475 (211> 900 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(877) <223> RXA01823 (400> 475 gcaacacgtt qatactgagc tgcacacgcc cgttggcctg PCTJIBOO/00926 gtgaccagtt tgctgggcgg ggtgtatttg atqtggcttt tqagccgaaa ggaggcataa atg ctg caa gcg cat Met Leu Gin Ala His ga t Asp ctg Le u tgc Cys ca a Gin acc Th r gcg Ala ccg Pro gtg Val1 tta Le u atg Met 150 a cg Thr ctg Le u ccg Pro t ca Se r aag Lys gtg Va 1 ggg Gly cat His 105 t cg Ser ga a Giu gct Ala ga c Asp ag t Se r ga a Glu acc Thr atc Ile gcg Ala gtg ValI ctg Leu ctg Leu ctt Leu cag Gin gta Val1 170 tac Tyr aga Arg gt t ValI a cg Th r aaa Lys 75 agc Ser ctg Le u gcg Al a t ca Se r ga a Giu 155 gcc Al a ggc Gi y gg C Gly ttg Leu cta Le u ca C His gt C Val t cg Se r gaa Glu ggc Gly 140 aca Thr cac His cga Arg agc Ser 30 gcg Ala ggg Gly ggg Gly gag Gi u t g Trp 110 ggc Gly ca g Gin att Ile ata Ile aat Asn atc Ile ttg Le u cg a Arg gtg Val1 ct g Le u tcg Ser atg Met cqc Arg ttg Leu tcC Ser 175 att Ile atc Ile Giy ga t Asp ctt Le u qtc Val1 aaa Lys cac His caa Gin ctt Le u 160 gtc Val 1 gta Val1 att Ile a ga Arg att Ile ccg Pro a gc Ser ga c Asp a cc Thr C gg Arg 145 ctc Leu Ctc Le u gaa Glu ggc Gly c tg Le u tcC Se r caa Gin cgc Arg ga t Asp cat His 130 gcg Ala gac Asp ga t Asp ggg Gly

CCC

Pro ctg Le u agc Se r

CCC

Pro ggg Gly gaa Gi u 115 gc t Ala tgg Trp gag Giu tta Le u 259 307 355 403 451 499 547 595 643 WO 01/00805 gac ctg cac caa cgt ggt cgc acc Asp Leu His Gin Arg Gly Arg Thr 1 185 aac atg gct gca cgg tat gcc acc Asn Met Ala Ala Arq Tyr Ala Thr 200 205 acc atc atc gac caa gga aaa ccc Thr Ile Ile Asp Gin Giy Lys Pro 215 220 ctc aaa gaa gtt ttt gac ctc gac Leu Lys Giu Val Phe Asp Leu AspJ 230 235 aac ggc cga cca ctc atc gtg ccc Asn Gly Arg Pro Leu Ile Val Pro 250 tgaaagaaac cgacaaccta ctg <210> 476 <211> 259 <212> PRT <213> Corynebacterium glutamicum PCTIIBOO/00926 gat ctg 691 Asp Leu gac ggc 739 Asp Giy gcg ctq 787 Ala Leu ccc aac 835 Pro Asn 245 <400> 476 Met Leu Gin Aia His Asp Leu Thr Leu Ser Tyr Gly Gly Arg Asn Ile 1 Vai Ilie Arg Ile Pro Se r Asp Thr Arg 145 Le u Gi u Giy Le u Ser Gin Arg Asp His 130 Aia Asp Le u Asn Ly s Met Pro Ar g 100 Al a Gi u Ile Pro 5 Se r Gly Pro Gly Tyr Tyr Ile His Ala Thr 165 10 Leu Cys Gin Thr 70 Al a Pro Val Leu Met 150 Th r Asp Gly Le u 55 Lys Pro His Ala Val1 135 Ala Phe Le u Ly s Gi y His Asp Gin Arg 120 Ser Le u Leu Pro 25 Ser Lys Val1 Gi y His 105 Ser Giu Ala Asp Giu Thr Ile Ala Val1 90 Leu Le u Le u Gin Val1 170 Arg Val1 Thr Lys 75 Ser Leu Ala Ser Giu 155 Ala Gly Le u Le u His Val1 Se r Giu Gly 140 Th r His Le u Lys Gly Ile Thr Gin Val1 125 Gly Asp Gin Ser Ala Gly Gly Giu Trp 110 Gly Gin Ile Ile Ile Leu Arg Val1 Le u Ser Met Arg Le u Se r 175 Ile Gly Asp Le u Val1 Lys His Gin Le u 160 Val WO 01/00805 Leu Asp Leu Val Leu His 195 Ala Met Arg 210 Leu Thr Lys 225 Leu Lys Asp Arg Asn Ser Cys 180 Asp Asp Ala Pro Ser Asp Leu His Gin 185 Leu Asn Met Ala Ala 200 Gly Thr Ile Ile Asp 215 Leu Leu Lys Giu Val 230 Asn Asn Gly Arg Pro 245 Gly Arg Thr Leu 190 Tyr Ala Thr His 205 Gly Lys Pro Glu 220 Asp Leu Asp Ala 235 Ile Val Pro Thr PCTIIBOO/00926 Ala Ile Ile Ile Glu Ile Leu Ile 240 Asp Arg 255 <210> 477 <211> 906 <212> DNA <213> Corynebacterium giutamicun <2 <221> CDS <222> (883) <223> RXA02767 <400> 477 gggaaaacgt ttcctcgccg cccacagaat cataaaaatt ccagtctaag ccctggcctt acgccaqtaa ggtqttaccc gcc Ala t cc Ser 9gC Gly cca Pro gat Asp aga Arg gtg Val1 ccg Pro acc Th r acc Th r ctg Le u ct g Le u Cgg Arg caa Gin 30 acc Thr gt g Val1 cgg Arg ccg Pro ttctgaggtt gtcatgggta atg cgc gaa cta gca Met Arg Giu Leu Ala 1 gag aaa ttg ctt ctc Glu Lys Leu Leu Leu cac tgg gcc gta ctt His Trp Ala Val Leu aag atc gca gcc acc Lys Ile Ala Ala Thr ctg ggg cat cgc ttt Leu Gly His Arg Phe atc ggc ctg gtg gac Ile Gly Leu Val Asp gaa att gtg ctg tcg Giu Ile Val Leu Ser 100 115 163 211 259 307 355 403 WO 01/00805 ggg tta acc gcc Gly Leu Thr Ala 105 tcg gag ttg gag Ser Glu Leu Glu 120 gcg cgt gcc gat Ala Arg Ala Asp 135 cgc acc ctg att Arg Thr Leu Ilie 150 ctt gat qaa ccc Leu Asp Glu Pro ctc agt gtg att Leu Ser Val Ile 185 gtg atg atc acc Val Met Ile Thr 200 atc ctc atg atc Ilie Leu Met Ilie 215 gaa gtg atg act Glu Val Met Thr 230 tcg ttg gaa act Ser Leu Glu Thr ggg Gly gc t Ala tgg Trp 140 gcg Ala gg C Giy ttg Leu gtc Val1 gcc Al a 220 aat Asn ttg Leu t tg Leu 125 gcc Al a ctg Leu ctt Le u cga Ar g gaa Glu 205 cgc Arg ttg Le u t cg Ser gtg Val1 130 qgc Gly acc Thr cg t Arg ggt Giy t cc Se r 210 9gg Gly ga c Asp PCTIBOOIOO926 tcg gct 451 Ser Ala atg aca 499 Met Thr aaa gcc 547 Lys Ala ctq ctg 595 Leu Leu 165 act ttg 643 Thr Leu 180 acg aca 691 Thr Thr aca gat 739 Thr Asp gtt tca 787 Val Ser tcq gtg 835 Ser Val 245 ctg cat 883 Leu His cgc agc cgg tgg ttc gcg ttc gat gct Arg Ser Arg Trp Phe Ala Phe Asp Ala 255 taaaaggggc tagttttaca caa <210> 478 <211> 261 <212> PRT <213> Corynebacterium glutarnicum <400> 478 Met Arg Glu Leu Ala Leu Asn Met Ala Gly Val Thr Val Arg Arg Gly 1 5 10 Glu Lys Leu Leu Leu Asp Asp Ile Ser Leu Ser Ilie Pro Gin Gly Ser 25 His Trp Ala Val Leu Gly Pro Asn Gly Ala Giy Lys Thr Thr Met Leu 40 Lys Ile Ala Ala Thr Leu Leu Tyr Pro Ser Glu Gly Thr Val Asp Ile 55 906 WO 01/00805 Leu Gly His Ilie Gly Leu Giu Ilie Val Trp Ser Ala 115 Leu Val Gly 130 Gin Gly Giu 145 Pro Thr Leu Giy Arg Glu Pro Giy Leu 195 Ala Ser Thr 210 Ser Gly Thr 225 Tyr Asp Met Phe Asp Ala Arg Phe Val Asp Leu Ser 100 Ser Ala Met Thr Lys Ala Leu Leu 165 Thr Leu 180 Thr Thr Thr Asp Val Ser Ser Val 245 Leu His 260 Gi y 70 Pro Gly Se r Ala Arg 150 Le u Le u Val Ile Giu 230 Arg Ly s Leu Giu Arg 135 Thr Asp Se r Met Le u 215 Val1 Asp Arg Ala 105 Gi u Asp Ile Pro Ile 185 Thr Ile Thr Thr Phe 90 Ser Arg Arg Ala Th r 170 Asp His Lys Pro Arg 75 Thr Asn Cys Tyr Arg 155 Thr Gly His Asp Giu 235 Le u Leu Leu Leu 125 Ala Leu Leu Arq G iu 205 Arg Leu Arcj Pro Leu 110 Met Asp Ile Asp Ala 190 Giu Ile G ly* PCT/1B00100926 Lys Thr Ala His Pro Arg Leu Glu Met Ser Ile Ser 160 Leu Pro 175 Ala Leu Ile Ala Leu Ala Ala Leu 240 Ser Leu Glu Thr Val Arg Ser Arg Trp Phe Ala 250 255 <210> 479 <211> 876 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <222> (853) <223> RXA02792 <400> 479 agaacaaagt gctgcccata ctcat~aact ttgccgaacc cccaaccccg ctggccgtcg atggcctaga aaaaatcatc gactttgtgg aaacccaccc atg atc gag gcc aca Met Ile Giu Ala Thr 1 cac cta cgc cac agt ttt ggc gac aac atc gtc atc gat gac gtc acc His Leu Arg His Ser Phe Gly Asp Asn Ile Val Ilie Asp Asp Vai Thr 15 WO 01/00805 ctc cat cta cct Leu His Leu Pro agt ggc aaa acc Ser Giy Lys Thr aat gaa ggg cac Asn Giu Giy His cgc aaa gac atc Arg Lys Asp Ilie tcc gac ctc ccc Ser Asp Leu Pro ccc cac caa aag Pro His Gin Lys 105 gtt aag gaa gca Val Lys Gin Aia 120 caa ttc ggc gca Gin Phe Giy Aia 135 cga gca ctc gta Arg Aia Leu Vai 150 aac cac ctc gac Asn His Len Asp gaa ctc aqc tca Gin Len Ser Ser 185 ggt gcc tac agc Giy Ala Tyr Ser 200 act caa gga acg Thr Gin Giy Thr 215 gtg tat gqc gtt Vai Tyr Gly Vai 230 g ca Ala acc Thr atc Ile g ca Aia a tg Met atg Met ctc Len ctt Len caa Gin atc Ile 170 agt Ser gac Asp ccc Pro cgt Arg ca c His ctg Len cac His aa a Ly s 75 acc Thr ttt Phe acc Thr tca Se r aac Asn 155 cg c Arg tcc Ser cac His tca Se r gt t Vali 235 Gi y ctg Len gta Vali 60 acc Thr gtc Vali gcc Ala cga Arg ggc Gly 140 gcc Aia tac Tyr atc Ile atc Ile gag Gin 220 gag Gin acc Th r cg t Arg 45 ga t Asp atg Met gct Ala Giy gt c Vali 125 gg t Gi y aca Th r ca g Gin atc Ilie atc Ile 205 gta Vai cg c Arg gtc Vali 30 gca Aila Gi y gcc Aila ga c Asp aac As n 110 ggc Gi y gaa Gin ca c His ca c His gt c Vali 190 ctc Len ttg Len ttt agc Ser cta Len gt t Val1 gta Vali c tg Len 95 t ct Ser g cc Ala cgc Arg att Ile ga a Gin 175 ctc Len ctt Len acc Thr gac t tg Len tac Tyr cca Pro gt c Vali 80 gtc Val caa Gin att Ile caa Gin ctg Len 160 gtc Va i cac His ga c Asp cca Pro cta gtc Vali gga Giy cta Len atc Ilie tta Len gct Aia ca c His cgc Arg, 145 ctc Len ctt Len gac Asp caa Gin gag Gin 225 ggc Gly gg C Giy g ca Aila ata Ile caa Gin cta Len ga t Asp ct g Len 130 gtc Vai ga c Asp cac His ctc Len gg a Giy 210 cat His gat Asp ccc Pro ct g Len agc Ser gaa Gin Giy ca g Gin 115 gcc Aia ctc Len gaa Gin ctc Len aac Asn 195 cgt Arg t tg Len gaa Gin PCT/IBOO/00926 aac ggc 211 Asn Giy caa cca 259 Gin Pro ttg cac 307 Len His cat gac 355 His Asp aga ctc 403 Arg Len 100 tta ctc 451 Len Len gac cga 499 Asp Arg atc gca 547 Ile Ala ccc acc 595 Pro Thr 165 gtc cgc 643 Val Arg 180 ctc qca 691 Len Aia gtg gtt 739 Vai Val gaa cct 787 Gin Pro gtc cac 835 Val His 245 Phe Asp Len 240 ctt cgg Len Arg ttc aag cgt cac taggagtttt gttgaggagg tgg Phe Lys Arg His 250 WO 01/00805 <210> 480 <211> 251 <212> PRT <213> Corynebacterium giutamicum <400> 480 Met Ile Giu Ala Thr His Leu Arg His PCTJIBOO/00926 1 Ile Val Gi y Le u Ile Le u Ala His Arq 145 Le u Le u Asp Gin Giu 225 Gly Asp Giy Ala Ile Gin Le u Asp Le u 130 Vai Asp His Le u Gly 210 His Asp Leu Ly s Gly Asp Le u Gin Giu 120 Gi y Leu Le u Ser T yr 200 Gly Gi y Phe Pro Thr His Ile Pro Lys 105 Ala Ala Val1 Asp Ser 185 Ser Thr Val1 Lys Ile Ser Leu Val Vai Le u Se r Ala Arg Ile Giu 175 Le u Leu Thr Asp Vali Leu Tyr Pro Val Vali Gin Ile Gin Leu 160 Val His Asp Pro Le u 240 <210> 481 <211> 1161 <212> DNA <213> Corynebacterium giutamicum <22 0> WO 01/00805 <221> CDS <222> (1138) <z223> RXN02929 <400> 481 caggtgggga cctcgcgtat cccagctggt ggaagaaatc PCTIIBOO/00926 gcagcgcagt tgaatcagct tgcttcttct gaagctgtgc cggccgctgc ttaagttttc gtg ctg aag aga att Vai Leu Lys Arg Ile ttc Phe ttt Phe aca Th r ga t Asp gtc Val1 gta Val gg t Gly gga Gly ggt Gly cag Gin 150 gct Al a acg Th r aac Asn ctc Leu gtg Val1 gca Ala cag Gin att Ile ttt Phe tCt Ser ttt Phe gtt Val1 135 gga Giy ttt Phe gtg Val1 t gg T rp aa C Asn gt g Vali 9t 9 Vali

C

Ala gtc Val1 aga Arg gg C Gly tct S er 120 g cc Aia tca Ser gcc Ala 9 cg Ala atg Met ccc Pro ctg Leu att Ile atc Ile gga Gly aac Asn ctt Le u 105 gca Aia gca Ala tca Ser ag t Ser cgc Arg 185 t cg Ser tgg Trp ttt Phe aga Arg ttc Phe g ca Al a c9 Pro gg t Gly C cg Pro g ta Val acc Thr tcc Se r 170 gta Val1 atc Ile gtg Val1 t ca Ser cat His ttt Phe acg Thr 75 ctg Leu gtc Vali agc Ser ctt Leu acc Th r 155 atc Ile tat Tyr ttC Phe gct Al a gg t Gly ttg Le u ga t Asp 60 ctg Le u gct Ala.

acg Th r atc Ile gc c Ala 140 gtg Val1 cag Gin gtg Vali atc Ile acc Thr ttt Ph e agt Ser 45 atc Ile gct Al a ga t Asp gca Ala 9gc Giy 125 a cg Thr gtt Val1 acc Thr t 99 Trp gtg ValI geg Ala a gc Ser 30 999 Gly C 99 Arg att Ile cct Pro gtg Val 110 gtg Val1 ctg Leu att Ile tat Tyr a tg Met 190 9Ct Ala ttg Le u 15 ggt Giy ca 9 Gin Ct 9 Le u tct Se r tat T yr 95 att Ile att Ile a tg Met t t 9 Le u a t't Ile 175 ttg Leu gtg Val1 t cg Ser gtt Val1 ga c Asp cct Pro g~t Gly 80 ttg Leu gt t ValI 99t Gly gtg Val1 9C9 Ala 160 ca g Gin 99C Gly gtg Val1 1 gta Vali att Ile a cg Th r cga Arg gct Ala t tg Le u 99 C Giy gc a Aila ag t Ser 145 99 C Gly caa Gin aac Asn 9 Cg Ala 9tC Val1 ga t Asp ct C Le u att Ile tct Ser ggt Gl y ggt Gly 9 ca Ala 130 C99 Arg gt 9 Val1 Cga Arg ctc Le u qga Giy att Ile tta Le u acc Th r atc Ile t aC T yr 9 tg Vali acc Thr 115 ttt Phe gga Gly 9Cg Al a cac His aat Asn 195 cta Le u t tg Le u agc Se r CCt Pro gct Ala caa Gin tcc Ser 100 gt 9 Val1 gta Vali gta Vali gtt Vali atc Ile 180 gtc Vali t 9C C ys 999 Gly ccc Pro Cga Ar9 gt Giy Aia gca Al a Ct 9 Le u ggt Gly gga Gly gct Ala 165 ga t Asp acc Thr gCg Al a 115 163 211 259 307 355 403 451 499 547 595 643 691 739 WO 01/00805 WO 0100805PCTIBOO/00926 gCC Ala gat Asp 230 ggc Gly t cc Ser ct a Leu t 99 Trp atg Met 310 gca Ala CCa Pro tcC Ser cg C Arg gtg Val1 250 999 Gly ccg Pro ttc Phe ga a Glu tta Le u 330 t gc Cys aca Thr 235 gca Ala ttt Phe 999 Gly ctC Le u ctt Le u 315 rtt gcc Al a 220 ttg Le u acc Thr gtg Val1 cat His gt g Val 300

CCC

Pro att 210 gac gtg atg Asp Val Met 225 cca ggc ctc Pro Gly Leu 240 gct gca gtg Ala Ala Val gtt ccg cac Val Pro His ctg cca ctg Leu Pro Leu 290 acc gca ggg Thr Ala Gly 305 gtq aca gct Val Thr Ala 320 acc agc aga Thr Ser Arg gct Ala gta Val1 gta Val1 gcc Ala 275 tct S er cga Arg gca Ala caa Gin gtt Val1 cgc Arg tCC S er 260 Ctg Leu ttc P he aca Thr ctc Leu cga Arg 340 787 835 883 931 979 1027 1075 1123 1161 Phe Ilie Leu Arg agt gCt Ser Ala 345 taagtggcga tcattgaatg cga <210> 482 <211> 346 <212> PRT <213> Corynebacteriwri giutamicum <400> 482 Val Leu Lys Arg Ile Phe Leu Asn Pro 1 5 Val Val Ile Leu Gly Phe Val Val Leu Ile Asp Leu Ser Pro Thr Ala Val Ile Thr Leu Thr Pro Arq Asp Gln Ala Ile Arg Ilie Ile Ala Gly Val Ile Val Gly 70 Ala Ser Tyr Gin Ala Val Phe Arg Asn Trp 10 P he Arg Phe Ala Pro ValI Ser His Phe Thr 75 Leu Leu Giy Gin Le u Ser Tyr Ser Val1 Asp Pro Giy Le u WO 01/00805 PCTIBOOIOO926 Ile Val Leu Giy Ala Ser 145 Gly Gin As n Ala Vali 225 Gly Ala Pro Pro Ala 305 Thr S er Gly Gly Al a 130 Arg Val1 Arg Leu Giy 210 Met Leu Val1 His Le u 290 Gly Ala Arg Val1 Thr 115 Phe Gly Ala His Asn 195 Leu Ala Val1 Val1 Ala 275 Ser Arg Ala Gin Ser 100 Val1 Vai Vai Vai Ile 180 ValI Cys Val1 Arg Se r 260 Le u Phe Th r Leu Arg 340 Ala Gly Ser Gly Leu Gly Vai Thr Ala Val L eu Giy Gly Ala 165 Asp Thr Ala Gly Ile 245 Ile Arg Val1 Le u Gly 325 Val1 Gly Gly Gin 150 Ala Thr Asn Ala Asp 230 Gly S er Leu T rp Met 310 Al a Pro Phe Val1 135 Giy Phe Val1 Trp Val1 215 Val1 Ile Gly Ile Gly 295 Ala Pro Lys Ser 120 Ala Ser Ala Ala Met 200 Ile Gi u ValI Le u Va 1 280 Aia Pro Phe Arg 105 Ala Ala Ser Ser Arg 185 Ser Met Al a Ile Ile 265 Gly Ile Gin Phe Se r 345 Pro Val1 Thr Ser 170 Val1 Ile Ser Arg Val1 250 Gly Pro Phe Giu Leu 330 Ala Ile Ala 140 Val Gin Val1 Ile Ala 220 Leu Thr Val1 His Val1 300 Pro Ile Gly 125 Thr Val1 Thr Trp Val1 205 Arg Gly Leu Gly Arg 285 Leu Val1 Le u <210> 483 <211> 1095 <212> DNA <213> Corynebacteriun glutamicun <220> <221> CDS <222> (1072) <223> FRXA01235 WO 01/00805 <400> 483 ttctgaaget gtgccggccg ctgcttaagt tttcgtgetg PCTIBOOIOO926 aagagaattt teetcaaeee ctgggtggct aecgcgttgt cggtagteat tttggggttt gtg gtg etg tit tca Val Val Leu Phe Ser ggt Gly ttg Leu ga t Asp Ctg Leu gct Al a aeg Thr atc Ile gce Ala g tg Val1 eag Gin 150 gtg Val1 atc Ile gcc Ala ttg Le u ttt Ph e a gt Ser ate Ile get Ala gat Asp g ca Al a g gc Gly a cg Thr gtt Val1 135 ace Thr t gg Trp gt g Val agg Arg ggC Gly age Ser ggg Gly cgg Arg at t Ile cc t Pro gtg Val1 gt g Val etg Leu 120 att Ile tat Tyr atg Met gct Ala ttg Leu 200 gte Val1 qgt Giy ca g Gin ctg Leu tet Ser tat T yr att Ile att Ilie 105 atg Met ttg Le u att Ile ttg Leu gtg Val1 185 tta Leu ga t Asp gt t Va 1 gac Asp ect Pro ggt Gly t tg Le u gtt Val1 gg t Gly gtg Val geg Ala ca 9 Gin gg C Gly 170 gtg Val gac Asp eca Pro att Ile acg Thr ega Arg get Ala ttg Leu 75 gge Gly gca Ala agt Ser g ge Gly eaa Gin 155 aae As n g eg Ala g tg Val1 Gly ga t Asp etc Leu att Ile get Ala 60 ggt Gly ggt Gly gea Ala egg Arg gt g Val1 140 cga Arg etc Le u gga Gly a tg Met etc Leu tta Leu ace Thr ate Ile 45 tac T yr gtg Val1 ace Thr ttt Phe gga Gly 125 gcg Ala eac His aat Asn eta Leu get Ala 205 gt a ValI age eec aca Ser Pro Thr 15 eet cga gat Pro Arg Asp 30 get ggt gte Ala Gly Val eaa geg gta Gin Ala Vai tee gca ggt Ser Ala Gly 80 gtg etg gga Vai Leu Gly 95 gta ggt ggt Val Giy Gly 110 gta gga eag Vai Gly Gin gtt get get Vai Ala Ala ate gat aeg Ile Asp Thr 160 gte aee aae Val Thr Asn 175 tge geg gee Cys Ala Ala 190 gtt ggt gat Val Gly Asp ege att gge Arg Ile Gly 1 gea Ala eag Gin att Ile ttt Phe tet Ser t tt Phe gtt Val1 gga Gi y ttt Phe 145 gtg Val t gg Trp gt g Vai gtg Val gtg Val1 gee Ala gte Val1 aga Arg gge Gly tet Se r gee Ala tea Se r 130 gee Ala geg Al a atg Met ate Ile gaa Giu 210 att Ile ate Ile gga Gi y aae Asn ett Le u gea Ala g ea Ala 115 tea Se r agt Ser eg e Arg teg Ser a tg Met 195 gee Ala a ga Arg tte Phe gea Ala e eg Pro gg9t Gly ecg Pro 100 gt a Val1 aee Thr tee Ser gta Val ate Ile 180 tee Ser ege Arg eat His ttt Phe a eg Th r etg Leu gte ValI age Se r ett Le u aee Th r ate Ile tat Tyr 165 tte Phe tge Cys aea Th r 115 163 211 259 307 355 403 451 499 547 595 643 691 739 787 att gte ate gtg gea Ile Val Ile Val Ala WO 01/00805 PCT/IBOO/00926 215 220 225 acc ctt ggt aca gct gca gtg gta tcc att tcc ggt ctc atc ggg ttt 835 Thr Leu Gly Thr Ala Ala Val Val Ser Ile Ser Gly Leu Ile Gly Phe 230 235 240 245 gtg ggc atc att gtt ccg cac gcc ctg cgc cta att gtt ggc ccg ggg 883 Val Gly Ile Ile Val Pro His Ala Leu Arg Leu Ile Val Giy Pro Gly 250 255 260 cat cgg att tta ctg cca ctg tct ttc gta tgg ggt gcc att ttc ctc 931 His Arg Ile Leu Leu Pro Leu Ser Phe Val Trp Gly Ala Ile Phe Leu 265 270 275 gtg ttg gca- gat acc gca g99 cga aca ttg atg gct cct cag gaa ctt 979 Val Leu Ala Asp Thr Ala Gly Arg Thr Leu Met Ala Pro Gin Giu Leu 280 285 290 ccc gtg ggt gtq gtg aca gct gca ctc ggc gca ccq ttc ttc tta ttt 1027 Pro Val Gly Val Val Thr Ala Ala Leu Gly Ala Pro Phe Phe Leu Phe 295 300 305 att ttq cgc aga acc agc aga caa cga gtt cca aaa agg agt qct 1072 Ile Leu Arg Arg Thr Ser Arg Gin Arg Vai Pro Lys Arg Ser Ala 310 315 320 taagtggcga tcattgaatg cga 1095 <210> 484 <211> 324 <212> PRT <213> Corynebacterium giutanicun <400> 484 Val Val Leu Phe Ser Giy Phe Ser Giy Vai Ile Asp Leu Ser Pro Thr 1 5 10 Ala Vai Ile Arg His Leu Ser Gly Gin Asp Thr Leu Thr Pro Arg Asp 25 Gin Ala Ile Phe Phe Asp Ile Arg Leu Pro Arg Ile Ile Ala Gly Val 40 Ile Val Gly Ala Thr Leu Ala Ile Ser Gly Ala Ala Tyr Gin Ala Val 55 Phe Arg Asn Pro Leu Ala Asp Pro Tyr Leu Leu Gly Val Ser Ala Gly 70 75 Ser Gly Leu Gly Val Thr Ala Val Ile Val Gly Gly Thr Val Leu Gly 90 Phe Ser Ala Pro Ser Ile Gly Val Ile Gly Ala Ala Phe Val Gly Giy 100 105 110 Val Ala Ala Val Leu Ala Thr Leu Met Val Ser Arg Gly Val Gly Gin 115 120 125 Gly Ser Ser Thr Thr Val Val Ilie Leu Ala Gly Vai Ala Val Ala Ala 130 135 140 WO 01/00805 Phe Ala Ser Ser 145 Val Ala Arg Val Trp Met Ser Ile 180 Val Ile Met Ser 195 Vai Giu Ala Arg 210 Ile Val Ile Val .225 Gly Leu Ile Gly Ile Val Gly Pro 260 Giy Ala Ile Phe 275 Ala Pro Gin Giu 290 Pro Phe Phe Leu 305 Lys Arg Ser Aia Tyr Met Ala Leu 200 Val Gly Ile Ile Al a 280 Gly Ile Leu Val 185 Le u Asp Th r Ile Le u 265 Asp ValI Arg Leu Gly Met Leu 220 Val1 His Leu Gly Ala 300 Asp Th r 175 Ala Gly Ile Ile Arg 255 ValI Le u Gi y PCTfIBOO/00926 Thr 160 Asn Ala Asp Gi y Ser 240 Leu Trp Met Ala Ile Leu Arg Arg Thr Ser Arg Gin Arg Val Pro <210> 485 <211> 1197 <212> DNA <213> Corynebacteriun giutamicum <220> <221> COS <222> (101)..(1174) <223> RXN02794 <400> 485 gcgcccactc atcggcgagc ttcaggagat gaggttgatg ctccattgat aatttctttc gctaatagtc aaatgatcat ttgagtgtta gtgttttctc atg ctt ctt tcc gcc 115 Met Leu Leu Ser Ala 1 cgc aca cac acg agt ttc caa gaa ctt gga ctc aat gct agt cgg cgc 163 Arg Thr His Thr Ser Phe Gin Giu Leu Giy Leu Asn Ala Ser Arg Arg 15 aaa gca atc aac tgg aca ctg gca ctc act gtg gtg cta att gcc tcc 211 Lys Ala Ile Asn Trp Thr Leu Ala Leu Thr Val Val Leu Ile Ala Ser WO 01/00805 WO 0100805PCT/EBOO/00926 atg Met acg Thr tcc Ser t tg Leu atg Met gtg Val1 gtg Val1 g gc Gly 150 ggg Gly tac T yr gcc Ala ctt Leu att Ile 230 aac Asn ctc Le u ttt Phe t gg Trp gac Asp ctc Leu caa Gin aac As n ggC Gly 135 gca Al a cgc Arg atg Met gaa Glu 9C Al a 215 g cc Al a tcc Se r cgc Arg gtt Val1 acc Thr act Thr gct Ala gta Val t ca Se r 120 gc t Ala atg Met atc Ile ctc Le u 99c Gly 200 gca Ala ctg Le u 9gc Gly att Ile 99c Gly gt a Val1 gcc Ala gcc Al a ctg Leu 105 9gt Gly gga Gi y gca Al a t cc Ser tc t Ser 185 agc Ser t gg Trp g cg Ala ga t Asp ctc Leu 265 gtg Val1 att Ile ga c Asp att Ile gi c Val1 gcc Ala ttt Phe gct Ala tcg Se r 170 9 cg Ala cgc Ar 9 aat Asn ttg Le u gaa Glu 250 ctc Le u ctc Leu agc Ser gcc Ala 75 9 tg Val1 cga Arg agt Ser ggc G ly tcC Ser 155 acc Thr gca Ala tcC Ser ggg Gly ctc Leu 235 acc Thr ctg Leu atc Ile cac His 60 atc Ile Gly aac Asn tgc Cys ga t Asp 140 gga Gly cgc Arg aca Th r gtg Val1 ccq Pro 220 atg Met gca Al a gtg Val1 ggt Gly cat His att Ile 9 ca Ala atg Met ggt Gly 125 tac Tyr ttg Le u ttg Le u agc Se r ttg Le u 205 atg Met gtq Val1 ctt Le u atc Ile 30 gca Ala ctt Le u t gg Trp ggc Gi y ct 9 Le u 110 g cg Ala gcc Al a atc Ilie t tg Leu ttt Phe 190 ttc Phe g cg Ala t t9 Leu acc Thr acc Thr 270 tcc Ser ttt Phe ta c Tyr ct t Le u 95 gca Ala gcc Al a ct c Leu ttc Phe atg Met 175 ct c Le u tgg Trp atc Ilie ggt Gi y ttg Le u 255 tgc Cys ggg Gi y Gly atc Ilie 80 gcc Ala gac Asp gct Ala caa Gin ttc Phe 160 t cc Se r atc Ile ct g Le u atc Ile ccg Pro 240 gga Gi y ctg Le u acc Th r act Thr cgc Arg ct 9 Leu ccc Pro gcc Ala 9gc Gly 145 gtq Val1 gyc Gly ttC Phe ctt Le u ttc Phe 225 caa Gin gta Val1 ctg Leu 9 t9 Val1 ct a Le u cca Pro g9t Gly atc Ile 115 ctg Le u 9Cg Al a cgc Arg 9 cg Ala t cc Ser 195 tcc Ser atc Ile ga t Asp cc t Pro gga Gly 275 ttt tcc Phe Ser ggt ggc Gly Gly cgc gtC Arg Val 9CC atc Ala Ile 100 ctc gg9 Leu Gly ttc gga Phe Gly ttt ctc Phe Leu gca gcg Ala Ala 165 atc gga Ile Gly 180 gac tcc Asp Ser tta qqa Leu Gly gtg 99C Val Gly 9cc tta Ala Leu 245 gat. cgc Asp Arg 260 tcc atg Ser Met 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 gtt Val1 ctg Le u tcc Ser 310 cgc Arg ctc Leu tac Tyr WO 01/00805 gcc atg gcc ggc agc atc gga Ala Met Ala Gly Ser Ile Gly 280 285 gcc agg cqt ttt gtt agt gga Ala Arg Arg Phe Val Ser Gly 295 300 gcg ttg atg ggc gca att ttg Ala Leu Met Gly Ala Ile Leu 315 acc ctg ctt qcg ccc caa gag Thr Leu Leu Ala Pro Gin Glu 330 atc gga gca ccc ttc ctc ctg Ile Gly Ala Pro Phe Leu Leu 345 tgatttttaa ggaattatgc gta PCT/IBOOIOO926 ac 979 ctt Le u ctc Leu 305 gct Ala ggc Gly cg c Arg gtc Val1 290 atg Met ga t Asp atc Ile Cg g Arg 1027 1075 1123 1171 1197 <210> 486 <211> 358 <212> PRT <213> Corynebacterium giutamicum <400> 486 Met Leu Leu Ser Ala Arg As n Val1 Thr Th r Arg Leu Pro Al a Giy 145 Arg Al a Phe' Gi y Arg Ala 100 Le u Ph e Phe Arg Se r Ser Gly Vali Ile Gly Giy Le u Lys Met Thr Se r 70 Leu Met Vai Val1 Gly 150 Thr Al a Phe Trp Asp Leu Gin As n Giy 135 Ala Ser Phe Trp Thr Val Leu Ile Ser Asp Ala Ile Val Val Arg Ala Ser Phe Gly Ala Ser 155 Gin Le u Ile His Ile Gly Asn Cys Asp 140 Gly Giu Ala Gly His Ile Al a Met Gly 125 Tyr Leu WO 01/00805 WO 0100805PCT/IBOO/00926 Val Ala Arg Ala Ala Gly Arg Ilie Ser Ser Thr Arg Leu Leu Met Ser 165 170 175 Gly Val Ala Ile Gly Tyr Met Leu Ser Ala Ala Thr Ser Phe Leu Ile 180 185 190 Phe Ser Ser Asp Ser Ala Giu Gly Ser Arq Ser Val Leu Phe Trp Leu 195 200 205 Leu Gly Ser Leu Gly Leu Ala Ala Trp Asn Gly Pro Met Ala Ile Ile 2.10 215 220 Phe Leu Ile Val Gly Ilie Ala Leu Ala Leu Leu Met Val Leu Gly Pro 225 230 235 240 Gin Leu Asp Ala Leu Asn Ser Gly Asp Giu Thr Ala Leu Thr Leu Gly 245 250 255 Val Ser Pro Asp Arg Leu Arg Ilie Leu Leu Leu Val Ile Thr Cys Leu 260 265 270 Leu Val Gly Ser Met Val Ala Met Ala Gly Ser Ilie Gly Phe Ile Gly 275 280 285 Leu Val Ile Pro His Leu Ala Arcj Arg Phe Val Ser Gly Lys His Arq 290 295 300 Leu Met Leu Pro Val Ser Ala Leu Met Gly Ala Ilie Leu Leu Ile Trp 305 310 315 320 Ala Asp Ile Ala Ala Arg Thr Leu Leu Ala Pro Gin Giu Ile Pro Ile 325 330 335 Gly Ilie Ilie Thr Ala Leu Ilie Gly Ala Pro Phe Leu Leu Ile Leu Val 340 345 350 Arg Arg Met His Thr Tyr 355 <210> 487 <211> 364 <212> DNA <213> Corynebacterium glutamicum <220> <221) CDS <222> (364) <223> FRXA01419 <400> 487 gcgcccactc atcggcgagc ttcaggagat gaggttgatg ctccattgat aatttctttc gctaatagtc aaatgatcat ttgagtgtta gtqttttctc atg ctt ctt tcc gcc 115 Met Leu Leu Ser Ala 1 cgc aca cac acg agt ttc caa gaa ctt gga ctc aat gct agt cgg cgc 163 Arg Thr His Thr Ser Phe Gin Glu Leu Gly Leu Asn Ala Ser Arg Arg 15 WO 01/00805 WO 0100805PCT[LBOO/00926 gca Ala ttt Phe tgg Trp gac Asp ctc Leu gca Ala ggt Gly cat His att Ile ctc Le u g ca Ala ctt Leu tgg Trp 211 259 307 355 <210> 488 <211> 88 (212> PRT <213> Corynebacteriun glutarnicum <400> 488 Met Leu Leu Ser Ala Arg Thr His Thr Ser 1 5 10 Asn Ala Ser Arg Arg Lys Ala Ile Asn Trp 25 Val Leu Ile Ala Ser Met Phe Val Giy Val 40 Thr Ser Val Phe Ser Thr Trp Thr Val Ile 55 Thr Glu Leu Gly Gly Ser Asp Thr Ala Asp 70 Arg Thr Pro Arg Val Leu Leu Ala Phe Gin Giu Leu Giy Leu Thr Leu Ala Leu Thr Vai Leu Ile Gly Ala Ser Gly Ser His His Leu Phe Gly Ala Ile Ile Trp Tyr Ile 75 <210> 489 <211> 744 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (721) <223> FRXA02794 <400> 489 tgaactcagg tgccagttgc ggtgcggccg ctgccttact gttcggagtg ggcgctggat ttggcgatta cgccctccaa gcagcgcgtt tctcggcgca atg gca gct tcc gga 115 WO 01/00805 WO 0100805PCT[IBOO/00926 Met Ala Ala Ser Gly ttg atc ttc Leu Ile Phe ttg ttg atg Leu Leu Met agc ttt ctc Ser Phe Leu ttg ttc tgg Leu Phe Trp atg gcg atc Met Ala Ilie gtg ttg ggt Val Leu Gly ctt acc ttg Leu Thr Leu atc acc tgc Ile Thr Cys 120 gga ttc atc Gly Phe Ile 135 gga aaa cac Gly Lys His 150 ttg ctc atc Leu Leu Ile gag att ccc Giu Ile Pro ctg att ctg Leu Ilie Leu 200 gta <210> 490 <211> 207 <212> PRT ttc Phe tdc Ser atc Ile ctg Leu atc Ile ccg Pro q ga Gly 105 ctg Le u gg C Gly cga Arg t gg Trp atc Ile 185 gtg Val1 ggc Gly ttc Phe ctt Le u ttc Phe caa Gin gt a Vali ctg Leu ctt Le u ctc Le u gct Ala 170 9gC Gly gcg Ala gta Val tcc Ser gg a Giy ctc Leu 75 ttg Le u tcc Se r gtg Val1 gtc Val1 atg Met 155 ga t Asp atc Ile cgc Arg g cg Ala tcc Ser tcc Se r 60 atc Ile ga t Asp cc t Pro gga Gly atc Ile 140 ctg Le u atc Ile atc Ile gcg ggg Ala Gly 15 gga tac Gly Tyr 30 tcc gcc Ser Ala gga ctt Gly Leu ggc att Gly Ile tta aac Leu Asn 95 cgc ctc Arg Leu 110 atg gtt Met Val cac .ctg His Leu gta tcc Val Ser gcc cgc Ala Arg 175 gca ctc Ala Leu 190 1 cgc atc Arg Ile atg ctc Met Leu gaa ggc Glu Gly gcc gca Ala Ala gcc ctg Ala Leu 80 tcc ggc Ser Gly cgc att Arg Ile gCC atg Ala Met gcc agg Ala Arg 145 gcg ttg Ala Leu 160 acc ctg Thr Leu atc gga Ile Gly t cc S er t ct Ser agc Ser t gg Trp gcg Ala ga t Asp ctc Leu gcc Ala 130 cg t Arg a tg Met ctt Leu gca t cg Ser gcg Ala cg c Arg aat As n t tg Le u gaa Glu ctc Le u 115 gg c Gly ttt Phe 9gC Gly gcg Al a

CCC

acc Thr g ca Ala tcc Ser ggg Gly ct C Leu acc Thr 100 ctg Le u agc Ser gt t Val1 g ca Ala

CCC

Pro 180 ttc cgc Arg aca Thr gtg Val1 ccg Pro a tg Met gca Ala gtg Val atc Ile ag t Se r att Ile 165 ca a Gin ctc 163 211 259 307 355 403 451 499 547 595 643 691 741 Aia Pro Phe Leu gtt cgc cgg atg Val Arg Arg Met cac acc tac His Thr Tyr 205 tgatttttaa ggaattatgc WO 01/00805 <213> Corynebacteriom glutamicum <400> 490 PCTIBOO/00926 Gly Arg Met Ala Ala Ser 1 Ile Leo Gly Ala Leu Gly Ile Met Arg 145 Leo Leu Gly Ser Ser Ser Trp Ala Asp Leu Ala 130 Arg Met Leo Ala Ser Ala Arg Asn Leu Glu Leu 115 Gly Phe Gly Ala Pro 195 Thr Ala Ser Gly Leo Thr 100 Leo Ser Val Ala Pro 180 Phe Gly 5 Arg Thr Va1 Pro Met Ala Va1 Ile Ser Ile 165 Gin Leo Leu Ile Phe Phe Val Ala Arg Ala Ala Leu Ser Leo Met 70 Val Leo Ile Gly Gly 150 Leo Glu Leo Leo Phe Phe 55 Ala Leo Thr Thr Phe 135 Lys Leo Ile Ile Met Leu 40 Trp Ile Gly Leo Cys 120 Ile His Ile Pro Leo 200 Ser 25 Ile Leo Ile Pro Gly 105 Leu Gly Arg Trp Ile 185 Val Gly Phe Leo Phe Gin 90 Va1 Leo Leo Leo Ala 170 Gly Arg Va1 Ser Gly Leu 75 Leo Ser Va1 Va1 Met 155 Asp Ile Arg Ala Ser Ser Ile Asp Pro Gly Ile 140 Leo Ile Ile Met Ile Asp Leo Val Ala Asp Ser 125 Pro Pro Ala Thr His 205 Gly Ser Gly Gly Leo Arg 110 Met His Val Ala Ala 190 Thr Tyr Ala Leu Ile Asn Leo Va1 Leo Ser Arg 175 Leo Tyr Met Glu Ala Ala Ser Arg Ala Ala Ala 160 Thr Ile <210> 491 <211> 1140 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1117) <223> RXN03079 <400> 491 gagcggggtt gctatcggcc gaaagtttag ggttttgttc aatctgttgg ttagtattgc ttgggtaaac aagtcataac aattttcatt aagggtcgtt ttg tcg cgc aca ggt 115 Leo Ser Arg Thr Gly 1 gtt tcg aaa aaa cca aag ctc acc gct cct gtt gtc atc atc ggc acc 163 WO 01/00805 WO 0100805PCTIIBOO/00926 Val Ser Lys Lys Pro Lys Leu Thr Ala Pro Val Vai Ile Ile Gly Thr ctc Le u g tg Val1 atc Ile gct Ala qa t Asp ttg Leu ttt Phe.

atc Ilie ttg Leu 150 tcc Ser acg Thr ggc Gly ggt Gly aca Thr 230 g cg Ala atc Ile aat Asn att Ile atc Ile gg t Gly ggt Gly ggc Gly gcg Ala atg Met 155 aac Asn ggc Gly ccc Pro aac Asn gtc Val1 235 gcc Ala gcc Ala gag Glu atc Ile atg Met qt g Val1 gcg Ala gca Ala atg Met 140 gct Ala gca Ala gat Asp att Ile ttg Le u 220 aa c Asn aca Thr ctc Le u ccc Pro gt g Val1 cac His qc t Ala caa Gin gc t Al a gca Ala 145 t tg Le u gag Giu acg Thr att Ilie ga t Asp 225 att Ile tcc Se r atq Met gtt Val gg t Gly aac Asn qt t Val1 tgg Trp gtg Val1 130 gat Asp ggt Gly ctt Le u t gg Trp cta Le u 210 tcc Ser ttg Leui ggc Gly ct g Leu gtc Val gcg Ala ccg Pro qca Ala acc Th r 115 gta Val1 ccg Pro gct Ala cga Arg ga a Gi u 195 gc t Ala aca Thr cta Le u acc Thr WO 01/00805 WO 0100805PCT/IBOO/00926 250 255 260 ttt gtt gga ttg gta gta ccc cac ctg gtg cgc att gtt tta ggt gcc 931 Phe Val Gly Leu Val Val Pro His Leu Val Arg Ilie Val Leu Gly Ala 265 270 275 gat cac cga gcg tta ctc ccg gcc gcc gcg att ttg ggc gcc acg ttt 979 Asp His Arg Ala Leu Leu Pro Ala Ala Ala Ile Leu Gly Ala Thr Phe 280 285 290 gtt atc gtt tcc gac act gtt gcc cgc aig atc ttc tcc ccc atc gtc 1027 Val Ilie Val Ser Asp Thr Val Ala Arg Met Ile Phe Ser Pro Ile Val 295 300 305 ttg caa aca ggc gtg gtg gtg gcg ttc att ggc tca cca att ttc ctt 1075 Leu Gin Thr Gly Val Val Val Ala Phe Ile Gly Ser Pro Ile Phe Leu 310 315 320 325 tat tta ctg ctc agc atg cgc aag cga cgc gga ttg ggg ctg 1117 Tyr Leu Leu Leu Ser Met Arg Lys Arg Arg Gly Leu Gly Leu 330 335 taaaaactca tgcctcaatt agt 1140 <210> 492 <211> 339 <212> PRT <213> Corynebacterium giutamicum '400> 492 Leu Ser Arg Thr Gly Val Ser Lys Lys Pro Lys Leu Thr Ala Pro Val 1 5 10 Val Ile Ilie Gly Thr Leu Val Leu Leu Ile Ile Ala Phe Thr Ala Ser 25 Leu Met Leu Gly Pro Val Thr Val Pro Leu Asn Giu Leu Ala Thr Asn 40 Pro Val Val Thr Asp Ilie Arg Ala Pro Arg Ilie Ile Ilie Ala Ala Leu 55 Val Gly Ala Ala Leu Ala Val Ser Gly Ala Ilie Met Gin Thr Val Phe .70 75 s0 His Asn Pro Leu Ala Asp Pro Gly Ile Val Gly Val Ser Ser Gly Ala 90 Ala Val Ala Ala Val Leu Ala Ilie Val Thr Gly Ala Ser Phe Phe Gly 100 105 110 Gin Trp Thr Val Pro Phe Ala Ala Phe Val Gly Ala Leu Val Thr Val 115 120 125 Ala Val Val Tyr Leu Ile Ala Ser Ser Arg Ala Met Asp Giy Arg Gly 130 135 140 Ala Asp Pro Ala Thr Leu Val Leu Val Gly Met Ala Ile Thr Ala Phe 145 150 155 160 WO 01/00805 WO 0100805PCTf[BOOIOO926 Leu Gly AlIa Val Ile Ser Ser Ala Thr Ala Asn Ala Pro Gin Asp Ser 165 170 175 Glu Leu Arg Ser Val Thr Phe Trp Leu Asn Gly Asp Leu Val Ser Arg 180 185 190 Thr Trp Giu His Val Gly Val Ala Ile Ile Pro Ile Ilie Val Gly Leu 195 200 205 Ilie Leu Ala Ile Gly Gly Ser Arg Asp Leu Asn Leu Leu Leu Leu Gly 210 215 220 Asp Ser Thr Ala Gin Thr Ser Gly Leu Asn Val Asn Arg Ala Arg Ile 225 230 235 240 Ilie Leu Leu Ala Leu Ala Ala Leu Leu Thr Ala Thr Ala Vai Ala Val 245 250 255 Ser Gly Thr Ilie Thr Phe Val Gly Leu Val Val Pro His Leu Val Arg 260 265 270 Ile Val Leu Gly Ala Asp His Arg Ala Leu Leu Pro Ala Ala Ala Ile 275 280 285 Leu Gly Ala Thr Phe Vai Ilie Val Ser Asp Thr Vai Ala Arg Met Ile 290 295 300 Phe Ser Pro Ile VaT Leu Gin Thr Gly Val VaT Val Ala Phe Ile Gly 305 310 315 320 Ser Pro Ilie Phe Leu Tyr Leu Leu Leu Ser Met Arg Lys Arg Arg Gly 325 330 335 Leu Gly Leu <210> 493 <211> 1140 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(1117) <223> FRXA02865 <400> 493 gagcggggtt gctatcggcc gaaagtttag ggttttgttc aatctgttgg ttagtattgc ttgggtaaac aagtcataac aattttcatt aagggtcgtt ttg tcg cgc aca ggt 115 Leu Ser Arg Thr Giy 1 gtt tcg aaa aaa cca aag ctc acc gct cct gtt gtc atc atc ggc acc 163 Val Ser Lys Lys Pro Lys Leu Thr Ala Pro Val Val Ile Ile Gly Thr 15 ctc gtc ttg ttg atc atc gcc ttc acc gct tcc ctc atg ctg ggt ccc 211 Leu VaT Leu Leu Ile Ile Ala Phe Thr Ala Ser Leu Met Leu Gly Pro 30 wo 01/00805 WO 0100805PCTJIBOO/00926 gtg Val1 atc Ile gct Ala gat Asp ttg Leu ttt Phe atc Ile ttg Leu 150 t cc Ser a cg Th r 9gc Gly ggt Gly aca Th r 230 gcg Ala a cq Thr cg t Arg gtC Val ccc Pro gcg Ala gcg Al a gct Ala 135 gta Val1 agc Ser ttt Phe gtt Val1 t cC Se r 215 tct Ser gca Ala gtt Val1 gca Ala t cc Ser ggc Gi y att Ile gcc Ala 120 ag t Ser ct g Le u g cc Ala tgg T rp gca Ala 200 cgc Arg gga Gly ct g Leu cca Pro cca Pro gg t Gi y att.

Ile gtc Val1 105 ttC Phe tcc Se r gt C Val1 act Thr ctc Le u 185 ata Ile ga t Asp ctc Leu ci c Le u t tg Leu cgc Arg g cg Ala gig Val1 acc Th r gig Val1 cg c Arg 9qC Gly gcg Al a 170 aac As n atc Ile ctg Leu aac Asn acc Thr 250 aat As n at Ile atc Ile 75 gg t Gly ggi Gi y Gly gcg Ala atg Met 155 aac As n ggc Gly ccc Pro aac Asn gi c Val1 235 gcc Ala gag Glu atc Ile 60 a ig Met gig Val1 g cg Al a gca Ala a tg Met 140 gct Ala gca Ala gat Asp at Ile ttg Leu 220 aac Asn aca Thr ctt Leu 45 atc Ile ca g Gin tcc Ser agt Se r ttg Leu 125 gati Asp atc Ilie cca Pro ctg Leu aic Ile 205 tig Leu cgc Arg gct Al a gca Al a gca Al a acg Thr t cc Ser ttc Ph e 110 gi c Val ggc Gi y act Thr caa Gin gt a Val1 190 gt Val ctg Leu g ca Ala gtt Val1 acc Thr gca Ala gtg Val1 ggi.

Gi y 95 ttt Phe a cg Th r cgt Arg gcc Ala gat Asp 175 tct Ser ggg Gi y ctg Leu cgc Arg gcg Ala 255 aac Asn t tg Leu ttt Phe 80 gca Ala ggc Gi y gtg Val1 gg C Gly ttt Phe 160 tct Se r cgg Arg tig Leu gg t Gi y atc Ile 240 gt c ValI ccc gtt Pro Val gtg ggt Val Gly cac aac His Asn gct gtt Ala Val caa tgg Gin Trp gc t gtg Ala Val 130 gca gat Ala Asp 145 ttg ggt Leu Gly gag ctt Giu Leu acg igg Thr Trp att cta Ile Leu 210 gat tcc Asp Ser 225 att ttg Ile Leu tcc ggc Ser Gly gtc acc Val Thr gcg gcg Ala Ala ccg ttg Pro Leu gca gct Ala Ala 100 acc gtt Thr Val 115 gta tat Val Tyr ccg gcc Pro Ala gct gtt Ala Val cga tcc Arg Ser 180 gaa cat Giu His 195 gct atc Ala Ile aca gcg Thr Ala cta gca Leu Ala acc att Thr Ilie 260 ga t Asp ctg Le u g cg Ala gtc Val cct Pro t tg Leu a cg Thr att Ilie 165 gig Val1 gtg Val1 Gly caa Gin ctt Le u 245 a cg Thr 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 ttt gtt gga ttg gta Phe Val Gly Leu Val 265 gta ccc cac ctg gtg cgc att gti tta ggt gcc Val Pro His Leu Val Arg Ile Val Leu Gly Ala WO 01/00805 gat cac cga gcg tta ctc ccg gcc Asp His Arg Ala Leu Leu Pro Ala 280 285 gtt atc gtt tcc gac act gtt gcc Val Ilie Val Ser Asp Thr Val Ala 295 300 ttg caa aca ggc gtg gtg gtq gcg I Leu Gin Thr Gly Val Val Val Ala 1 310 315 tat tta ctg ctc aqc atg cgc aag Tyr Leu Leu Leu Ser Met Arg Lys 330 taaaaactca tgcctcaatt agt <210> 494 <211> 339 <212> PRT <213> Corynebacterium giutamicum gcc acg ttt Ala Thr Phe ccc atc gtc Pro Ile Val att ttc ctt Ile Phe Leu 325 ctg Leu 'T/EBOO/00926 979 1027 1075 1117 1140 <400> 494 Leu Ser Arg Thr Gly Val Ser Lys Lys Pro Lys Leu Thr Ala Pro Val 1 Val1 Le u Pro Val1 His Ala Gin Al a Al a 145 Leu Ilie Met Val1 Gly As n Val1 Trp Val1 130 Asp Gly Ile Leu Val1 Ala Pro Al a Thr 115 Val1 Pro Ala Gly Thr Gly Pro Thr Asp Ala Leu Leu Ala Ala Val 100 Val Pro Tyr Leu Ala Thr Val Ilie 165 Le u Val1 Ile Ala 70 Asp Le u Phe Ile Leu 150 Se r Val1 Thr Arg 55 Val1 Pro Ala Al a Ala 135 Val Ser Leu Val 40 Ala Ser Gly Ile Ala 120 Se r Leu Ala Le u 25 Pro Pro Gly Ile Val 105 Ph e Ser Val Th r Ile Le u Arg Ala Val1 90 Th r Val Arg G ly Ala 170 Ile Asn Ile Ile 75 Gly Gly Gly Ala Met 155 As n Ala Glu Ile Met Val1 Ala Ala Met 140 Ala Ala Phe Thr Leu Ala Ile Ala Gin Thr Ser Ser Ser Phe 110 Leu Val 125 Asp Gly Ile Thr Pro Gin Al a Thr Ala Va 1 Gly Phe Thr A rg Ala Asp 175 Ser As n Leu Phe Ala Gly Val1 Gly Phe 160 Ser Giu Leu Arg Ser Val Thr Phe Trp Leu Asn Gly Asp Leu Val Ser Arg WO 01/00805 Thr Trp Glu His 195 Ilie Leu Ala Ile 210 Asp Ser Thr Ala 225 Ile Leu Leu Ala Ser Gly Thr Ile 260 Ile Val Leu Gly 275 Leu Gly Ala Thr 290 Phe Ser Pro Ile 305 Ser Pro Ilie Phe Leu Gly Leu Gly Gly Th r 230 Ala Phe Asp Val1 Le u 310 Tyr Val Ser 215 Ser Al a Val1 His Ile 295 Gin Le u Pro Asn ValI 235 Al a Val1 Le u Thr Val 315 Met Ile Leu 220 Asn Th r Pro Pro Val1 300 Val1 Arg Val1 Leu Ala ValI Leu 270 Ala Arq Phe Arg Gly Leu Arg Ala 255 Val1 Ala Met Ile Arg 335 PCTIIBOO/00926 Leu Gly Ile 240 Val1 Arg Ile Ile Gly 320 Gly <210> 495 <211> 1695 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (1672) <223> RXA00181 <400> 495 gtatattcag acacccacgt cgattagtgt tgtagttcac atctttgcac gtttgtcccc tacccaaaag gagaaacctc tgg T rp ggC Gi y Ala tca ttc Phe tat Tyr ggc Gly gat atc I le cg c Arg aac As n tgg gtt Val1 aag Lys cct Pro ctg agtgcttatt ttttatttgt atg agc gat aac acc Met Ser Asp Asn Thr 1 gtg atg gtg ctc atc Val Met Val Leu Ile gac gac tac atg ctc Asp Asp Tyr Met Leu atg tcc gca ggt gcc Met Ser Ala Gly Ala ccc ggc gcg ctg ttt WO 01/00805 PCT/IBO0/00926 Gly Trp Leu Leu Met Gly Leu Pro Gly Ala Leu Phe 60 Ser gtc Val gca Ala gaa Gi u ctt Leu att Ile gtg Val 150 att Ile gct Ala ttg Le u ga t Asp gga Gly 230 gca Ala cca Pro aag Lys ggt Gly Asp acc Thr tgg T rp atc Ile cgc Arg gtg Val 135 tat Tyr gtc Val1 gtg ValI atc Ile att Ile 215 att Ilie gca Ala cac His caa Gin gca Ala Met ggc Gi y gcg Aila t cg Ser ga t Asp 120 ttc Phe t gg T rp gca Ala tct Ser atc Ile 200 tgg Trp gg C Giy atc Ile att Ile gg t Gly 280 acc Th r Se r atg Met aac Asn gcc Ala 105 aaa Ly s ttc Phe gag Giu ggt Giy tat Tyr 185 gtg Val1 agc Se r aat Asn att Ile gtg Val1 265 cg t Arg ttt Phe gag Glu 75 a tg Met tca Ser cgc Arg ttc Phe acg Thr 155 aca Thr ga c Asp gtc Val1 gca Ala acc Thr 235 aac Asn cg t Arg atc Ilie gca Ala t tg Leu tgg Trp atc Ile g cg Ala tac Tyr 140 ttt Phe gtg Val gca Ala atg Met aac Asn 220 tac Tyr tta Leu ttc Phe ggt Giy att Ile tgg Trp gtg Val acc Thr ctt Leu 125 att Ile ggt Giy ct g Leu gtt Val1 gca Al a 205 tct Se r ttc Phe gig t Gi y atg Met att Ilie 285 atc Ile gtc Val1 80 cg t Arg t ca Se r att Ile Gly tat T yr 160 ttc Phe act Thr gca Ala tac Tyr atc Ile 240 ctt Le u cg C Arg a tg Met gt t Val1 gg a Giy ctt Le u ttc Phe gca Al a atg Met 145 ct a Le u att Ile atc Ile ct g Le u 9gc Gly 225 a gt Ser gga Gly aca Thr ata Ilie ttc Phe a cc Thr tct Se r gag Giu 115 cta Le u gct Al a gg t Gly gga Gly ttc Phe 195 aac As n cac His att Ile ttc Phe gct Ala 275 t gc Cys g ca Al a att Ile tat Tyr 100 aac As n att Ile gg t Gly atg Met ttc Phe 180 ttc Phe ccc Pro acc Thr tct Se r ggc Gly 260 gaa Giu ctg Le u caa Gin gg t Gly t cc Ser cga Arg atc Ile gga Gly gcc Ala 165 ctt Leu t cg Se r atg Met gat Asp gca Ala 245 cag Gin gca Al a att Ile aac Asn 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 WO 01/00805 WO 0100805PCTJIBOO/00926 ccc gac 9CC Pro Asp Ala 310 gat ctt 9CC Asp Leu Ala act gct gtt Thr Ala Val gtc acc gca Val Thr Ala 360 gac tcg ctg Asp Ser Leu 375 atc att ctg Ile Ilie Leu 390 tcc atc ctc Ser Ilie Leu ttt ggt ccg Phe Gly Pro gcc ggc gct Ala Gly Ala 440 tgg ggt atg Trp Gly Met 455 ttc gcc ttg Phe Ala Leu 470 gaa ccc tc Glu Pro Ser gct gcc gct Ala Ala Ala cag aag ct Gin Lys Leu 520 <210> 496 <211> 524 aac atc act Asn Ile Thr 315 cgg atg ctg Arg Met Leu 330 etc gcg 9CC Leu Ala Ala 345 tet tcc ctg Ser Ser Leu agc gaa c9c Ser Glu Arg 9c9 ate att Ala Ilie Ilie 395 gga ttg gtg Gly Leu Val 410 ate ata ctt Ilie Ilie Leu 425 atc ice ggc Ilie Ser Gly icc cca cia Ser Pro Leu gea acc ate Ala Thr Ilie 475 gaa gaa ate Giu Giu Ile 490 gtg gaa tea Val Glu Ser 505 g ac Asp ttc Phe aic Ilie att Ile acc Thr 380 gca Ala g ga Gly get Ala atg Met a gc Ser 460 gt 9 Val etc Leu aac Asn aca agg gct Thr Arg Ala cac His atg Met gag Glu 365 C ig Leu 9 ca Aila ttt Ph e agc Ser att Ilie 445 ga t Asp atg Met aac Asn gag cca Pro tct Ser 350 gac Asp aic Ile 9CC Al a ge 9 Ala ctt Le u 430 act Thr acg Th r gte Val1 gag Glu gat ttg Le u 335 a cc Thr ctg Leu a ig Met atg Met igg T rp 415 tat Tyr ggi Gly tg Leu gt Val1 Phe 495 gtt tat gag Tyr Glu 320 atc gct Ile Ala atg teg Met Ser ctg aag Leu Lys cig tet Leu Ser 385 gct att Ala Ilie 400 gca gga Ala Gly tgg aag Trp Lys get att Ala Ilie tat gaa Tyr Glu 465 gtt tee Val Ser 480 gaa acc Glu Thr gat tie Ser gga Gi y t ca Ser gta Val1 370 Cg i Arg aac Asn ttc Phe eg t Arg gtc Val1 450 aic Ile ctc Le u 9CC Ala get Ile ct Le u ca 9 Gin 355 gtt ValI 9CC Al a cc 9 Pro ggc Giy ct C Le u 435 icc Ser atc Ilie ctg Le u aag Lys gac tcc atc ttc cta.

Phe att Ile 340 ctg Le u aaa Lys aca Th r tct Se r tct Se r 420 aac Asn atc Ile cca Pro aca Thr gati Asp 500 gca Al a Leu 325 ctg Leu ctg Le u aag Ly s gte Val1 gat Asp 405 9 ca Al a 9CC Al a geg Al a ggt Gly aag Lys 485 ct Leu get Ala 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1695 Glu Asp 510 Val Asp Phe Ala Asp teg aaa gaa agi Ser Lys Glu Ser taagccttag agggaaceaa acg WO 01/00805 <212> PRT <213> Corynebacterium glutamicum <400> 496 Met Ser Asp Asn Thr Trp Phe Ile Ile PCTIBOOIOO926 1 Val Asp Met Pro Gly Le u Ph e Ala Met 145 Le u Ile Ile Le u GI y 225 Se r Gly Thr Val1 Tyr Al a Ala Thr Ser Glu 115 Le u Ala Gly Gly Phe 195 Asn His Ile Phe Ala 275 Leu Met Gly Leu Ile Tyr 100 Asn Ile Gly Met Phe 180 Phe Pro Thr Ser Gly 260 Giu 5 Ile Leu Ala Ph e Gi y Ser Arg Ile Gi y Ala 165 Le u Ser Met Asp Ala 245 Gin Ala Gly Ala Ser Val1 70 Ala Glu Leu Ile Val1 150 Ile Ala Leu Asp Gi y 230 Ala Pro Lys Tyr Gly Asp Thr Trp Ilie Arg Val 135 Tyr Val1 Val1 Ile Ile 215 Ile Aia His Gin Trp Arg 40 Met Gly Ala Se r Asp 120 Phe Trp Al a Ser I le 200 Trp Gi y Ile Ile Gly 280 Ala 10 T yr Leu Gly Ser T rp 90 Asn Ser thr Ser Val1 170 Thr Pro Phe Pro Gly 250 Vali Arg Ile Arg Asn T rp Glu 75 Met Ser Arg Phe Thr 155 Thr Asp Val1 Ala Th r 235 Asn Arg Ile Val1 Lys Pro Leu Leu T rp Ile Ala T yr 140 Phe Val1 Ala Met Asn 220 Tyr Le u Phe Giy Ile Thr Phe Leu Trp Val Thr Le u 125 Ile Gly Le u Val1 Ala 205 Ser Phe Giy Met Ile 285 Tyr Giu Val1 Met Ile Ala Leu 110 Arg Se r Gi y Tyr Gln 190 Tyr Asn Ser Trp Al a 270 Ser Met Lys Ala Gly Ala Pro Pro Ile Ser Asp Thr 175 Gly Ph e Asp Met Gi y 255 Leu Trp Le u Tyr Al a Leu Val Arg S er Ile Gi y Tyr 160 Ph e Thr Ala Tyr Ile 240 Leu Arg Met Ile Ile 290 Cys Leu Ile Gly Thr Phe Thr Aia Ile Ser Thr Val WO 01/00805 WO 0100805PCT/IBOO/00926 Phe Phe Ala Gin Asn Pro Asp Ala Asn Ile Thr Asp Thr Arg Ala Tyr 305 310 315 320 Giu Ser Ile Phe Leu Asp Leu Ala Arg Met Leu Phe His Pro Leu Ile 325 330 335 Ala Gly Leu Ile Leu Thr Ala Val Leu Ala Ala Ile Met Ser Thr Met 340 345 350 Ser Ser Gin Leu Leu Val Thr Ala Ser Ser Leu Ile Giu Asp Leu Leu 355 360 365 Lys Val Val Lys Lys Asp Ser Leu Ser Giu Arg Thr Leu Ile Met Leu 370 375 380 Ser Arg Ala Thr Val Ile Ilie Leu Ala Ile Ile Ala Ala Ala Met Ala 385 390 395 400 Ile Asn Pro Ser Asp Ser Ile Leu Gly Leu Val Gly Phe Ala Trp Ala 405 410 415 Giy Phe Gly Ser Ala Phe Gly Pro Ile Ile Leu Ala Ser Leu Tyr Trp 420 425 430 Lys Arg Leu Asn Ala Ala Gly Ala Ile Ser Gly Met Ile Thr Gly Ala 435 440 445 Ilie Val Ser Ile Ala Trp Gly Met Ser Pro Leu Ser Asp Thr Leu Tyr 450 455 460 Glu Ile Ile Pro Gly Phe Ala Leu Ala Thr Ile Val Met Val Val Val 465 470 475 480 Ser Leu Leu Thr Lys Glu Pro Ser Glu Glu Ile Leu Asn Glu Phe Giu 485 490 495 Thr Ala Lys Asp Leu Ala Ala Ala Val Glu Ser Asn Glu Asp Val Asp 500 505 510 Phe Ala Asp Ala Ala Gin Lys Leu Ser Lys Glu Ser 515 520 <210> 497 <211> 1476 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (1453) <223> RXA00591 (400> 497 gcaacgccac aggcaaaatc caaaagaaca tcctgcgaga cttcaccatc cccgtttcat aaacccccca acgtcacttt gaaaacactt ggagaacgca atg act aca tct tcc 115 Met Thr Thr Ser Ser 1 aca gct tcg ccg atc gct gaa tta caa aac ctc agc ccg aag caa aga 163 WO 01/00805 WO 0100805PCTIiBOO/00926 Th r a aa Lys atc Ile ttc Phe 9CC Al a gga Gly ct t Leu ctt Le u atc Ile qga Gly 150 ttc Ph e gga Gly ttC Ph e c tc L eu ttc Ph e 230 gtc Val1 Pro tcc Ser tat Tyr gt g ValI gga Gly ttc Phe tcc Ser 105 agc Ser cgc Arg ct C Leu tcc Ser g cg Al a 185 t 99 Trp ggc Gly 9 ca Ala ttg Leu Ile Ala Glu Leu Gin Asn Leu'Ser PrQ Lys Gin Arg 15 Cgg Arg ga c Asp ttc Phe acc Th r gqa Giy a tg Met qct Al a atc Ile a tg Met ttc Ph e 170 ctc Leu 99 t Giy ttg Le u 9C9 Aia ca 9 Gin CgC Arg ttc Phe ttt Phe ttc Ph e 75 cac His ct C Le u ga a Giu tg c Cys gCg Ala 155 acc Thr ggt Giy tgg Trp gtt Vali 9CC Aia 235 gt t Val1 g9 Ala Ctg Leu acc Thr 9CC Ala ttc Phe atc Ile atq Met ca a Gin 140 Ctc Leu aac Asn acc Thr C9C Ar9 atc Ilie 220 9CC Aia Ctt Leu att Ile tta Leu aat Asn 9 Ca Aia gg t Giy at9 Met atc Ile 125 99C Gly gag Glu 9 Ct Aila gca Aila atc Ile 205 CgC Arg gaa Giu CgC Arg ata Ile 30 tat T yr Ct C Leu ggt Giy 9g C Asp 99 t Gi y 110 gga Giy atc Ile cac His ggt Gly t ct Se r 190 cca Pro gCg Aila aaa Lys cga Arg tcc Ser 9CC Al a ga t Asp tat Tyr Cgt Arg 95 gtg ValI agc Ser gct Ala t Ca Ser 9 Ca Ala 175 9ct Ala ttc Phe aaa Lys 9CC Ala cca Pro ta C T yr 9CC Aia Ct 9 Leu 9ct Ala ga Gly tcc Ser qqC Gly ggt Gly 145 t CC Ser acc Th r ctc Leu Ct C Leu agc Se r 225 cca Pro 9 Ca Al a ttg Leu tcc Se r gC t Al a a 99 Ar 9 C9 C A rg aca Thr 9C9 Al a 130 99 C Gly aag Lys 99 C Gi y acc Th r tct S er 210 ga a Giu acc Th r Ctg Leu 99C Gly gC9 Ala 9 ga Gly C Ca Pro aag Lys tgc C ys 115 gta Val.

gaa Giu aag L ys gct Ala cag Gin 195 ttc Phe tca Ser gaa Glu atc Ile tcc S er a Cg Thr acc Thr Ctt Leu aa 9 Lys 100 atc Ile atc Ile tgq Trp C9 C Arg gca Ala 180 gag Giu gtt Val cca Pro C9 t Arg ttg Leu a C9 Thr gta Val1 att Ile ggt Giy atg Met 99C Gly Ct 9 Leu 99C Gly gga Gly 165 Ctq Leu Caa Gin ctg Leu Ctg Leu a aa L ys 245 a CC Thr 211 259 307 355 403 451 499 547 595 643 691 739 787 835 883 WO 01/00805 WO 0100805PCT/IBOO/00926 atg ctt ggc Met Leu Gly ttc tcc atc Phe Ser Ilie 280 ctc tac gcc Leu Tyr Ala 295 ctc ttc ggt Leu Phe Gly 310 atc gcg ctc Ile Ala Leu ctc acc tca Leu Thr Ser ctc gca ctc Leu Ala Leu 360 gaa caa ttc Giu Gin Phe 375 cag ttg gcc Gin Leu Ala 390 agc ctc tac Ser Leu Tyr gtC ttc ctc Val Phe Leu acc aga gaa Thr Arg Glu 440 250 ggc gca tca gga Gly Ala Ser Gly 265 aqc tac gca aca Ser Tyr Ala Thr ttc gca gtc gcc Phe Ala Val Ala 300 cqc gta tcc gac Arg Val Ser Asp 315 gta ctg ttc gtg Val Leu Phe Val 330 gac aac tgg ttc Asp Asn Trp Phe 345 cac gcc atg ctt His Ala Met Leu qga acc tcC gcg Gly Thr Ser Ala 380 acg ctc atc ggt Thr Leu Ile Gly 395 gcg gga-cca ggc Ala Giy Pro Gly 410 qca acq atg tcc Ala Thr Met Ser 425 tca aaa gac cac Ser Lys Asp His ttC Ph e caa Gin 285 t Ca Ser ctc Leu gCC Ala atc Ile tac Tyr 365 cq C Arg gca Al a Gi y cta Leu ga t Asp gga Gly 270 tcc Ser gtg Val1 ttc Phe ta C T yr ctg Leu 350 ggc Gly tac Tyr gga Gly gga Gly gtg Val1 430 ct t Leu 255 ctt Leu 99C Gly ttc Ph e gga Gi y ctg Leu 335 ct C Leu cca Pro a ca Th r ttc P he acc Thr 415 tcc S er tct S er caa Gln atC Ile tct Ser Cg C Arg 320 CCg Pro t Cg Ser Cta Le u gq C Gl y acc Th r 400 t Ct Ser atc Ile act Thr aCC Th r qtC Val1 atc Ile atc Ilie 325 atg Met 9 Cg Al a t CC Ser tac Tyr qCt Al a 405 atc Ile atc Ile 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1453 1476 taagggqacg atagggtcaa ttt <210> 498 <211> 451 <212> PRT c213> CorynebaCterium glutamicum <400> 498 Met Thr Thr Ser Ser Thr Ala Ser Pro Ile Ala Glu Leu Gln Asn Leu 1 5 10 WO 01/00805 WO 0100805PCTIBOOIOO926 Ser Tyr Ala Leu Ala GI y S er Gly Gly 145 Ser Thr Leu Leu Ser 225 Pro Ala Val Giu Va 1 305 Pro Lys Leu Gly Ser Ala Ala Gly Arg Pro Arg Lys Thr Cys 115 Ala Val 130 Gly Glu Lys Lys Gly Ala Thr Gln 195 Ser Phe 210 Glu Ser Thr Glu Leu Ile Leu Leu 275 Arg Ser 290 Phe Phe Gin Ser Thr Th r Leu Lys 100 Ile Ile Trp Arg Ala 180 Giu Val Pro Arg Leu 260 Ser Se r Val1 Arq Lys Ser Glu Ser Arg Arg Ala Ile Ile Ser Ser 25 Thr Val1 Ile Gly Met Gly Leu Gly Gly 165 Leu Gin Leu Leu L ys 245 Thr Thr ValI Ilie Ilie 325 Ile Phe Ala 70 Gly Leu Leu Ile Gly 150 Phe Gly Phe Leu Ph e 230 Val1 Met Ph e Leu Leu 310 Giu Pro 55 Ala Ala Val1 Val1 Met 135 Ala Al a Thr Leu Ilie 215 Al a Pro Leu Ser Tyr 295 Phe Phe 40 Al a Tyr Ile Leu Pro 120 Leu Ala Ala Phe Ser 200 Val Ala Leu Gi y Ilie 280 Ala Gly Tyr Val1 Gi y Phe Ser 105 Ser Arg Leu Ser Ala 185 Trp Gly Ala Leu Gly 265 Ser Phe Arg Asp Ph e Thr Gly 90 Met Al a Ile Met Ph e 170 Leu Gly Le u Al a Gin 250 Al a Tyr Al a Val1 Le u 330 Ph e Ph e Phe 75 His Leu Glu Cys Ala 155 Th r Gly T rp Val Al a 235 Val1 Ser Al a Val1 Ser 315 Ph e Leu -Leu Thr Asn Ala Ala Phe Gly Ile Met Met Ilie 125 Gin Gly 140 Leu Glu Asn Ala Thr Ala Ar Ile 205 Ilie Arg 220 Ala Giu Leu Arg Gly Phe Thr Gin 285 Ala Ser 300 Asp Leu Val Ala Tyr Leu Gly Asp Gly 110 Gly Ile His Gly Ser 190 Pro Ala Lys Arg Gly 270 Ser Val1 Phe T yr Ala Ala Asp Pro Tyr Leu Arg Leu Val Ala Ser Met Ala Val Ser Asp 160 Ala Pro 175 Ala Val Phe Leu Lys Val Ala Lys 240 Pro Lys 255 Leu Gin Gly Ile Phe Ser Gly Arq 320 Leu Pro 335 Arg Pro Val Met Ilie Ala Leu Val WO 01/00805 Ala Phe Phe Arg Met Leu Thr Ser Asp Asn Trp 340 345 Ala Phe Thr Ile Ala Leu Ala Leu His Ala Met 355 360 Ala Ala Phe Ilie Ser Giu Gln Phe Gly Thr Ser 370 375 *Ala Ser Leu Gly Tyr Gin Leu Ala Thr Leu Ilie 385 390 395 Pro Thr Ile Leu Ala Ser Leu Tyr Ala Gly Pro 405 410 Val Thr Pro Val Ilie Val Phe Leu Ala Thr met 420 425 Ilie Ala Ilie Ala Ilie Thr Arg Giu Ser Lys Asp 435 440 Tyr Glu His 450 <210> 499 <211> 1635 <212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <222> (101)..(1612) <223> RXA01629 <400> 499 caggaagaag ttaacaccgc ccaggggtgc gttggatgat tccgttatgc actcataaga tatgacgaga ggttttactc Leu Pro Thr Ph e Th r 415 Ser Ser PCTIBOO/00926 Ser Leu Gly Thr 400 Ser Ile Th r tca Ser aa c As n gt g Val1 a cg Th r cag Gin aaa Ly s gt t ValI gg t Gi y gtc Val1 ttg Le u aag Lys gtt Val aac Asn a cg Thr ttg L eu aaa Lys ccc Pro ttc Phe atg Met gCC Ala aa c As n aag Lys tgg Trp gta Val1 60 ttt Phe ga a Giu atc Ile tac Tyr 45 ttt Phe gg t Gly gatcatctac aaacaaacat gtg agc ccg att cgc Val Ser Pro Ile Arg 1 aca gtc gat gac gtc Thr Val Asp Asp Val att aaa ggc act gtg Ile Lys Gly Thr Val att tat ggt tat ttg Ilie Tyr Gly Tyr Leu ctg ccg caa gaa tgg Leu Pro Gin Glu Trp tct tat tta gtt cgc Ser Tyr Leu Val 'Arg wo 01/00805 WO 0100805PCTIBOO/00926 CCg Pro Ca 9 Gin Ctg Leu gtg Val1 gaa Glu 150 cga Arg ttt Phe cac His ttc Phe cgt Arg 230 9 ca Ala 9 Ct Ala att Ile CCt Pro 9 Ct Ala 310 9gt Gi y gt t ValI 99C Gly 120 ttg L eu 9Ct Ala ggt Gly 9 C9 Ala tat Tyr 200 act Thr CCt Pro gtt Val gtt Val1 g ca Ala 280 tat T yr a CC Th r ggC Gi y ttg Leu 105 ttg Leu tac Ty r 99 t Gi y tt C Phe ggt Gly 185 ggC Gly 9 Cg Al a gag Giu gaa Glu atc Ile 265 9Cg Ala cta Leu gtg Val1 C tg Leu tac Tyr Ct 9 Leu Ct 9 Leu 9c9 Ala ttc Phe 170 9ct Al a 9 ca Al a atc Ile acc Thr aag Lys 250 C9 C Arq acc Thr gag Glu CCg Pro gtt Val1 gta Val1 C C9 Pro ttg Leu acc Th r 155 9 ga Gly t Cg Ser act Thr ccg Pro cct Pro 235 ga c Asp cac His aac As n gag Glu att Ile 315 ctg Leu acc Thr a cg Th r aag Lys 140 acc Thr gct Ala gtc Val1 9CC Al a ctg Leu 220 9g Ala act Th r ca c His acc Thr cag Gin 300 Ctg Leu ggt Gly atg Met 9 C9 Al a 125 atq Met tat Tyr ttc Ph e 9t 9 ValI atg Met 205 99C Gi y ttt Ph e ga a Glu t99 Trp 9CC Al a 285 atc Ile gt t Val ccg Pro 9CC Ala 110 9 Cg Al a gtg ValI gtt Val1 cta Leu 9gg Al a 190 gag Giu atc Ile gag Giu ga t Asp cgt Arg 270 ggt Gly ggt Gly gt t Val1 Ctt Leu 95 atg Met t cg Ser ca g Gin gCt Al a ga t Asp 175 att Ile ga t Asp att Ile aac Asn cct Pro 255 cca Pro tac Tyr Ct 9 Leu atg Met 9gC Gly atg Met ata Ile gga Gly gag Glu 160 atg Met act Th r ttc Phe g Cg Ala aac Asn 240 tat Tyr ctg Leu 9 C9 Ala cac His tcc Ser 320 ga t Asp 9CC Ala 99C Gi y ttt Ph e 145 ttt Phe ggt Gly acc Th r ggt Gly gtg ValI 225 caa Gin gca Al a Ct 9 Leu ct a Leu t CC Se r 305 ctg Leu aag Lys gt g Val1 gct Ala 130 t cg Ser gg Ala tCC Se r tgg Trp tgg Trp 210 tac Tyr gac Asp C9 C Arg att Ile acc Thr 290 g ca Al a Ctg Leu qtt Val1 tct Ser 115 tgg Trp act Thr C C9 Pro tac Tyr gtg Val1 195 cgt Arg ctg Leu gag Giu Ct 9 Leu ggt Gly 275 agt Ser t CC Ser ctg Leu 99C Gi y 100 a cg Th r g Ct Al a gg t Gly gat Asp Ctg Leu 180 acc Thr att Ile cgt Arg cca Pro 99 C Gly 260 att Ile tac Tyr 9 Ct Ala cca Pro cgC Arg g9 Al a t tg Le u ggt Gi y cg t Arg 165 99 C Gi y act Th r cct Pro act Th r aat As n 245 Ct 9 Leu 9 C9 Ala atg Met 9CC Al a ttt Phe 325 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 gt t Val1 gct Al a aac As n att Ilie ctg Leu 390 att Ilie ctg Leu ttc Phe t ca Ser gag Glu 470 ct t WO 01/00805 ggt atg tgg Gly Met Trp gtt gcg gca Val Ala Ala 345 acc gga acc Thr Gly Thr 360 cca acc ggt Pro Thr Gly 375 ttc cca acc Phe Pro Thr tcc gtg tcg Ser Val Ser ctg cag aag Leu Gin Lys 425 ttc tct gca Phe Ser Ala 440 cag aaq cca Gin Lys Pro 455 gcc gtg gag Ala Val Giu tcc cat atg agt Ser 330 acg Th r atc Ile ttg Le u g cg Al a ttg Leu 410 act Th r atc Ile ctg Le u atc Ile ccg ga c Asp ctg Leu.

Gi y tat Tyr tca Ser 395 ttc Phe 9gc Gly g ca Al a ctt Leu gtg Val 475 ttt cg c Arg atc Ile gcc Ala gtg ValI 380 cga Arg ggt Gi y ct g Leu gg t Gly qg c Gly 460 aag Lys cct gtg Val1 t tg Leu gta Val1 365 gca Al a ttc Phe ggt Gly gat Asp gtg Val 445 t ca Ser aac Asn gat cg c Arg 335 gt t ValI att Ile t cc Ser gga Gi y acc Thr 415 gt t Val ctg Le u cca Pro ga c Asp cc t Pro gcc Ala ct g Leu tct Ser 385 ggt Gi y ctg Leu g cg Ala ttc Phe gtg Val1 465 ga t Asp gt c Val1 ttc Phe tcc Ser 370 gcc Ala att Ile atc Ile ctc Leu atg Met 450 gaa Glu cca Pro tac Tyr ttg Leu 355 a tg Met ctg Leu tcc Ser acc Thr tac Tyr 435 acc Thr acc Thr aat As n gcg Aila 340 atc Ile gtt Val1 c ca Pro tac Tyr ca g Gin 420 atc Ilie gag Giu aag Lys att Ile PCTIBOOOO926 act 1123 Thr atg 1171 Met gct 1219 Ala gcg 1267 Ala aac 1315 Asn 405 ttc 1363 Phe atg 1411 Met tct 1459 Se r tct 1507 Ser gat 1555 Asp 485 aag 1603 L ys Leu Ser His Met Pro Phe Pro Asp 490 gaa aac gta Giu Asn Val 495 ggt gct gaa Gly Ala Glu 500 caa aac gca Gln Asn Ala taaccgtaaa gcccgctgca agg 1635 <210> 500 <211> 504 <212> PRT <213> Corynebacteriun giutarnicum <400> 500 Val Ser Pro Ilie Arg Ser Lys Lys Lys Ile Lys Asn Giu Pro Arg Leu 1 5 10 Thr Val Asp Asp Val Asn Vai Val Pro Pro Lys Lys Ile Arg Pro Ala 25 WO 01/00805 Ile Lys Giy Thr Ile Tyr Giy Tyr Leu Pro Gin Giu Ser Tyr Leu Val Asp Lys Vai Giy 100 Aia Vai Ser Thr 115 Giy Aia Trp Aia 130 Phe Ser Thr Giy 145 Phe Ala Pro Asp Gly Ser Tyr Leu 180 Thr Trp Vai Thr 195 Giy Trp Arg Ilie .210 Vai Tyr Leu Arg 225 Gin Asp Giu Pro Aia Arq Leu Giy 260 Leu Ilie Giy Ile 275 Leu Thr Ser Tyr 290 Ser Aia Ser Aia 305 Leu Leu Leu Pro Pro Val Tyr Aia 340 PCTIiBOO/00926 Giy. Vai Val Giy Asn Phe Met Giu Trp Tyr Asp Phe 40 Leu Trp Arq Arq Aia Leu Giy Arg 165 Gi y T hr Pro Thr Asn 245 Leu Aila Met Aia Phe 325 Thr Vai Thr 55 Leu Leu Leu Giy Lys Vai Ile Gly 120 Leu Leu 135 Tyr Ala Arq Gly Ala Aia Phe Tyr 200 Leu Thr 215 Ile Pro Val Val Gly Vai Val Ala 280 Val Tyr 295 Val Thr Giy Met Val Ala Met Thr Aia Aia Vai Met 75 Giy Leu Vai 90 Leu Tyr Val 105 Leu Leu Pro Tyr Leu Leu Giy Ala Thr 155 Phe Phe Giy 170 Gly Ala Ser 185 Gly Aia Thr Aia Ile Pro Giu Thr Pro 235 Giu Lys Asp 250 Ile Arg His 265 Aia Thr Asn Leu Giu Giu Vai Pro Ile 315 Trp Ser Asp 330 Aia Thr Leu 345 Thr Ile Gly Vai Phe Leu Th r Thr Lys 140 Thr Ala Vali Aila Le u 220 Al a Thr His Th r Gin 300 Le u Ar q Ile Ala Phe Gly Giy Met Aila 125 Met Tyr Phe Val Met 205 Giy Phe Giu T rp Aia 285 Ile Val Val Leu Val Thr Ph e Pro Aia 110 Ala Vali Val1 Leu Ala 190 Glu Ilie Giu Asp Arg 270 Gly Gly Vali Gi y Met 350 Leu Gin Aila Le u Met Ser Gin Aila Asp 175 Ile Asp Ile Asn Pro 255 Pro Tyr Leu Met Arg 335 Val1 Ile Gly Val1 Giy Met Ile Gly Glu 160 Met Thr Phe Aila Asn 240 T yr Leu Ala His Ser 320 Lys Pro Ala Ala Phe Leu Ilie Met Asn Thr Gly WO 01/00805 355 Leu Ser Met Vz 370 Ser Ala Leu Pi 385 Gly Ile Ser T5 Leu Ile Thr GI Ala Leu Tyr II 435 Phe Met Thr GI 450 Val Giu Thr L) 465 Asp Pro Asn II Val Gly Ala GI PCTIIBOO/00926 Al a Ala Asn 405 Phe Met Ser Ser Asp 485 Lys Pro 375 Ph e S e-r Leu Phe Gin 455 Ala Ser As n 360 Th r Pro Val1 Gin Se r 440 Lys ValI His Ala Gly Th r Ser Lys 425 Ala Pro Giu Met Ser Giy Thr 415 ValI Leu Pro Asp Glu 495 Ala Met 400 Pro Pro Le u Th r Gi u 480 Asn <210> 501 <211> 1509 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1486) <223> RXA02030 <400> 501 gcgcctaaca tctcattgta tgggattgac tctaataate ggtaattatt ggaatctatt attgtgtgag gcgataactc cac His ct a Leu ate Ile get Ala gag Gi u ga C Asp ace Thr gtt ValI cca Pro cgg Arg ttt Phe ttc Ph e ggt Gly gtt Val tat Tyr ect Pro tcetgtggaa aeategeaag gtg ace aca act gat Vai Thr Thr Thr Asp 1 ggg cag aeg gca ace Gly Gin Thr Ala Thr gca gca gee tee ace Ala Ala Ala Ser Thr geg tac get geg gca Ala Tyr Ala Ala Ala gee aat gac aac eca Ala Asn Asp Asn Pro 115 163 211 259 307 WO 01/00805 PCT[IBOO/00926 a cc Thr gct Al a gga Gly acc Thr gca Al a gg c Gly 150 aa c Asn ttt Ph e ctt Le u tgg Trp tgg Trp 230 ga t Asp tcc Se r acc Thr aa c As n cca Pro aaa Lys t tg Le u 120 gcg Ala gaa Glu cac His ttc Phe cat His 200 ctg L eu cgt Arg ggc Gly 99c Gly ttc Ph e 280 ctg Leu ctc Leu gct Ala 105 atc Ilie ctg Leu tgg Trp cqc Arg ttc Phe 185 ca g Gin cca Pro ttc Phe aag Lys c ct Pro 265 tac Tyr t tg Leu g gt Gly acC Thr gg C Gly ttg Leu tct Ser gct Ala 170 t tg Leu ga c Asp ttc Phe t ca Ser aag Lys 250 gt t Val1 ct g Leu gga Gly gca Ala 75 t ca S er t tg Leu ctg Leu 9 Cg Ala gg C Gly 155 cg t Arg gcc Ala 99C Gi y ct g Le u ctg Le u 235 gtg ValI gtg Val1 gt t Val1 t cg Se r atc Ile atc Ile ctg Le u ctc Leu 140 gct Al a gct Ala aa t Asn ga t Asp tct Se r 220 gag Giu aag Lys ca g Gin ac Th r ttt gcc acc Phe Ala Thr att ttc ggg Ile Phe Gly 95 ggt tca ctg Gly Ser Leu 110 cct act tat Pro Thr Tyr 125 atg cgt ttc Met Arg Phe gcg ttg ctg Ala Leu Leu gca atq tgg Ala Met Trp 175 ggt ttc atg Gly Phe Met 190 ctt cac ggc Leu His Gly 205 tcg gcg atc Ser Ala Ilie gaa act cca Glu Thr Pro tct ccg Ctc Ser Pro Leu 255 gct act ttg Ala Thr Leu 270 acg tgg att Thr Trp Ile 285 .ctg tct att Leu Ser Ile ttt Phe 80 cac His ctg Le u ggt Gly tgc Cys gc t Al a 160 cca Pro ctC Leu gcg Al a atg Met gt g ValI 240 aag Lys atc Ile ct g Le u cc g Pro ggt Gly ttc Phe acc Thr ca g Gin cag Gin 145 gg t Gly cag Gin att Ile ttc Phe atc Ilie 225 ttc Ph e g ag Giu atg Met tcg S er tac T yr 305 ct t Leu ggt Gly atg Met gt C Val1 130 ggc Gly gaa Giu ctg Leu ct g Leu atg Met 210 gca Ala a ag Lys ctg Leu ctg Leu tat Tyr 290 ttt Phe gcg Al a ga c Asp ggc Gly 115 gga Gly ctg Leu aac As n *ggt Gly gt c Val1 195 acc Thr qt t Val1 cag Gin ttc Phe t CC Ser 275 gg c Gi y gag Glu ttc Ph e cg t Arg 100 att Ile atc Ile Gly gcg Al a g ca Ala 180 ggt Gly tgg Trp ggc Gi y gct Ala aag Lys 260 act Thr atc Ile ttc Phe gtt Val gtg ValI g cc Ala att Ile ct t Leu gaa Glu 165 C cg Pro gtg Val 99 t Gly ctg Leu gtt Val1 245 act Th r tat Tyr ggt Gly t tq Leu 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 aat cgc agc acc Asn Arg Ser Thr 295 aac ggc Asn Gly 300 WO 01/00805 WO 0100805PCTJIEBOO/00926 ca g Gin 310 t ca Ser gct Ala ga t Asp ggc Gly cc 9 Pro 390 tac Tyr acg Thr atc Ile t tg L eu 9gC Gly tct S er c ca Pro a tg Met 375 gag Glu aat Asn tgg T rp atc Ile acc Thr 315 ga t Asp ct C Leu acc Thr ggt Gly acc Thr 395 atc Ilie gag Giu att Ilie atc Ilie gt g Val1 gq C Gly aag Lys ctg Leu 380 aac Asn ctc Le u ttt Phe acc Thr gtg Val1 tgg T rp ttt Phe acc Th r 365 atc Ile gtc Vai ggt Gly tca Ser ttt Phe 44.5 ttc qcc Phe Ala 320 Cgt aaa Arg Lys 335 ctg acg Leu Thr gtg ttc Val Phe gga ccc Gly Pro tac acg Tyr Thr 400 gct att Ala Ilie 415 gcg tac Ala Tyr gcg gtq Ala Val atc Ilie aac As n ttt Phe atc Ile atg Met 385 ggc Gi y gca Al a gtc Val1 ct g atg Met acc Thr aat Asn ttc Phe 370 t cg Ser t cc S er ccg Pro ggc Gly a cq atc Ile ctg Leu ctg Leu 355 ttg Leu g ca Ala gga Gly ttc Phe tat T yr 435 a tg cct Pro acc Thr 340 ctt Leu ttc Phe att Ile atc Ile atc Ile 420 tac Tyr aaq gtg Val1 325 ttg Leu ctc Leu gtg ValI t tg Leu gcc Ala 405 g ca Ala ctc Leu gaa 1075 1123 1171 1219 1267 1315 1363 1411 1459 1506 1509 Leu Thr Met Lys Glu 450 aac aaa aac cac gac ctc cga gag gtc Asn Lys Asn His Asp Leu Arg Glu Val 455 460 a cg <210> 502 <211> 462 <212> PRT <213> Corynebacterium glutamicum <400> 502 Val Thr Thr Thr Asp His Ser Thr Glu 1 5 Gly Gin Thr Ala Thr Leu Val Ile Asp 25 Ala Ala Ala Ser Thr Ile Gly Thr Thr 40 Ala Tyr Ala Ala Ala Ala Val Val Val 55 taaaagattt ttgcttatcg Asn Pro Ser Asp Pro Gly Lys Thr Lys Arg Arg Val Glu Phe Tyr Asp Phe Tyr Pro Ser Leu Phe Phe Pro WO 01/00805 PCTIBOOIOO926 Ala Asn Asp Asn Pro Thr Val Asn Leu Leu Ala Ser ?he Ala Thr Phe 70 75 Gly Leu Ala Phe Val Ala Arq Pro Leu Gly Ser Ilie Ile Phe Gly His 90 Phe Gly Asp Arg Val Gly Arg Lys Ala Thr Leu Ile Gly Ser Leu Leu 100 105 110 Thr Met Gly Ilie Ala Thr Ilie Leu Ile Gly Leu Leu Pro Thr Tyr Gly 115 120 125 Gin Vai Gly Ile Ilie Ala Pro Ala Leu Leu Ala Leu Met Arg Phe Cys 130 135 Gin Gly Leu Gly Leu Gly Gly Giu Trp Ser Gly Ala Ala Leu Leu Ala 145 150 i55 160 Gly Giu Asn Ala Glu Asn Thr His Arq Ala Arg Ala Ala Met Trp Pro 165 170 175 Gin Leu Gly Ala Pro Phe Gly Phe Phe Leu Ala Asn Gly Phe Met Leu 180 185 190 Ile Leu Val Gly Val Leu Ala His Gin Asp Gly Asp Leu His Gly Ala 195 200 205 Phe Met Thr Trp Gly Trp Arq Leu Pro Phe Leu Ser Ser Ala Ile Met 210 215 220 Ile Ala Val Gly Leu Trp Val Arg Phe Ser Leu Glu Glu Thr Pro Vai 225 230 235 240 Phe Lys Gin Ala Val Asp Gin Gly Lys Lys Val Lys Ser Pro Leu Lys .245 250 255 Giu Leu Phe Lys Thr Ser Pro Gly Pro Val Val Gln Ala Thr Leu Ile 260 265 270 Met Leu Ser Thr Tyr Thr Leu Phe Tyr Leu Val Thr Thr Trp Ile Leu 275 280 285 Ser Tyr Gly Ilie Gly Asn Arg Ser Thr Gly Asn Gly Leu Ser Ile Pro 290 295 300 Tyr Phe Giu Phe Leu Gin Leu Gin Leu Ala Thr Ile Vai Phe Phe Ala 305 310 315 320 Ilie Met Ilie Pro Val Ser Gly Trp Leu Ala Asp Val Trp Gly Arq Lys 325 330 335 Asn Thr Leu Thr Leu Ala Ser Val Leu Leu Leu Gly Phe Gly Leu Thr 340 345 350 Phe Asn Leu Leu Leu Asp Pro Glu Thr Ala Thr Lys Thr Thr Val Phe 355 360 365 Ile Phe Leu Phe Val Gly Met Ser Ilie Met Gly Leu Ile Phe Gly Pro 370 375 380 WO 01/00805 Met Ser Ala Ilie Leu Pro Glu Leu Phe Pro Thr Asn 385 390 395 Gly Ser Gly Ilie Ala Tyr Asn Val Ser Ser Ile Leu 405 410 Ala Pro Phe Ilie Ala Thr Trp Leu Val Ser Glu Phe 420 425 Val Gly Tyr Tyr Leu Ilie Ilie Val Thr Ala Ile Thr 435 440 Leu Thr Met Lys Glu Asn Lys Asn His Asp Leu Arg 450 455 460 <210> 503 <211> 870 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (847) <223> RXA00186 <400> 503 agaagcccag cattttgttt agctcacctc taaagctcct gat PCTIIBOO/00926 Vai Arg Tyr Thr 400 Gly Ala Ala Ile 415 Ser Val Ala Tyr 430 Phe Val Ala Val 445 Glu Val ctggata cgaggactcc tgcqgaaaac cqtqtggcta qtqaagctac ccqgcatctc atg ggg gag aaq act Met Gly Glu Lys Thr tct S er ata Ile ttc Phe a cc Thr ga t Asp tgc Cys aac As n tat Tyr ccg Pro 9gc Gly ga t Asp cg t Arg gtc Val1 cta Leu 105 gtt Val cac His gqa Gly att Ile qca Ala acg Th r gt g Val cac His ccc Pro t qq Trp gcc Al a ccg Pro 75 gaa Glu gca Al a ga t Asp aac Asn gtt ValI gtt Val1 ga a Glu gct Ala gtc Val1 gt t ValI ga c Asp ttg Leu gct Ala cat His gtc Val 9gc Gly ctg Leu ggq Gi y 30 99g Gly tca Ser cct Pro ct c Leu ggg Gly 110 atc Ile 15 ccg Pro gac Asp ggc Gly ttt Phe aat Asn 95 gac Asp aag Lys ct t Leu ctt Le u gcc Al a ctc Leu 80 ggt Gly a gc Ser 1 ggc Gly cct Pro ga a Giu ctg Leu gct Al a gaa Glu gcc Ala gtg ValI gtc Val t ca Ser tgt Cys gca Ala ct c Leu gg t Gly cca Pro ttt Ph e Val1 atc Ile ttg Leu gcc Al a ggc Gi y 115 g tg Val atc Ilie gat Asp agt Ser ga t Asp agt Ser 100 aat Asn Cg g Ar g tac Tyr t cc Ser gtg Val ga t Asp gct Al a att Ile WO 01/00805 WO 0100895PCTn~BOO/00926 g cg Ala ctt Leu att Ilie 150 a cg Thr gaa Giu agc Ser gtg Val1 ttc Phe 230 gtc atc gct caa cag ctg cgc gat cag atc acc cac caa gtt Vai Ile Ala Gin Gin Leu Arg Asp Gin Ile Thr His Gin Vai 125 130 gt g Vai ttt Ph e t ct Se r 170 att Ile atg Met t gg Trp ttc atg Met g cg Aia 155 ga t Asp ctg Leu tac T yr aca Th r ctg ga c Asp 140 ct g Le u ga a Glu cgt Arg ttc Phe qyt Giy 220 t ca gtg Val 145 a ag Lys atc Ile gaa Giy ctg Leu 225 ctc Leu att Ile agc Ser ca a Gin aaa Ly s ctg Le u 210 a cg Th r acc Thr tct S er ga c Asp gga Gi y 180 aa t As n tac Tyr aac Asn aat As n a tc Ile aag Lys 165 agt Ser g cg Ala aqc Se r t CC Ser tac Tyr 245 Phe Leu Ser Leu Giy Met Val ggg cca gag tat taagcaaaqc ttattcgcga agg Gly Pro Giu Tyr <210> 504 <211> 249 <212> PRT <213> Corynebacterium giutamicun <400> 504 Met Giy Glu Lys Thr Ser Vai Ala Tyr Val H 1 5 10 Gly Val Pro Val Arg Ile Tyr Asn Pro His P Pro Vai Phe Ile Tyr Phe His Giy Gly Gly T 40 Giu Ser Val Asp Ser Thr Val Arg Asp Ile A Leu Cys Ile Ser Val Asp Tyr Arg Arg Ala P Ala Ala Leu Asp Asp Cys Gin Val Vai Thr G 90 870 Val1 Asp Leu Ala His Val1 Ile Pro Asp Gly Phe Asn wo 01/00805 WO 0100805PCTIBOOIOO926 Glu Leu Ala Ser Ala Asn Gin His Leu Val Ala Ile ValI 145 Ly s Ile Gi u Gly Le u 225 Leut Gly T hr 130 Ile S er Gin L ys Leu 210 Th r Th r Gly 115 His Cys Ly s Ala Al a 195 Leu Leu Ala 100 Asn Gln Ser Asp Gi y 180 Asn T yr Asn Asn Ile ValI Ile Ly s 165 Ser Ala Ser Ser Tyr 245 Al a Le u Ile 150 Thr Glu Ser Val1 Phe 230 Gl y 105 Ile Pro Gi y Ile Se r 185 Ile Leu Leu Tyr Ala Val P he Ser 170 Ile Met Trp Phe <210> 505 <211> 468 <212> DNA (213> Corynebacterium giutamicum <220> <221> CDS <222> (101) (451) <223> RXA00187 <400> 505 gcttattcgc gaaggcatcc aaggaacgtg gggattcatt aggcattttc gggttgattt ccttcaccgg tctgggtgtt ttc Phe tac Tyr atc Ile ga c acc tcg Thr Ser tct gga Ser Giy atc gaa Ile Glu atg ggc a cq Thr aac Asn tac Tyr ctg q ca Ala atc Ile atc Ilie cag t gg T rp cct Pro acc Th r caa cttcaattcc cgttctacgc gtg atc tcc gga ttt Val Ile Ser Giy Phe 1 gtc atc gcg ttc ctc Val Ile Ala Phe Leu ggt ggc tcg aaa ttc Giy Gly Ser Lys Phe gtg gat ctt ggc gct Val Asp Leu Giy Ala agt gat ggc gcg acc 115 163 211 259 307 WO 01/00805 Asp Met Gly Leu Val Leu Gin Ala 60 aac ctg ctc att ccq ttc ttc gcg Asn Leu Leu Ile Pro Phe Phe Ala 75 atc aaa ttc agc caa gtg gtq gqc Ile Lys Phe Ser Gin Val Val Gly gtt atc gcc gtg atc tgc att tac Val Ile Ala Val Ile Cys Ile TyrI 105 taaaaagatg cttctcg <210> 506 <211> 117 <212> PRT <213> Corynebacterium giutamicum <400> 506 Vai Ile Ser Giy Phe Phe Thr Ser 1 Vai Ile Ala Phe Leu Tyr Ser Giy Giy Gly Ser Lys Phe Ile Ile Giu Val Asp Leu Gly Ala Asp Met Gly 55 Ser Asp Gly Ala Thr Asn Leu Leu 70 Leu Ala Asn Phe Lys Ile Lys Phe Pro Pro Val Leu Val Val Ilie Ala 100 Arg Ala Ser Ile Ile 115 PCTIBOOIOO926 Gin Met Ser Asp cca tat ttg gcc Pro Tyr Leu Ala 80 aca qtt ccg cct Thr Val Pro Pro 95 ttc ctg cga. gca Phe Leu Arq Aia Gly Ala Thr aat ttc aag Asn Phe Lys gtc ctc gtt Val Leu Val 100 tca atc att Ser Ile Ile 115 Tb r Asn T yr Le u Ph e Val Cys Ala Ile Ile Gin Ph e ValI Ile <210> 507 <211> 1329 <212> DNA <213> Corynebacterium giutaricum <220> <221> CDS <222> (101)..(1306) <223> RXA01667 <400> 507 tctcgqcqtt gatgatcatc cgcaatatca tgccgccgca agattccgct tttccagact 737 WO 01/00805 PCT/tBOO/00926 ttcgctcattc cttcctctat tactgctcgc catcatcggc atq att ttc; cgc; cct 115 Met Ile Leu Gly Pro 1 aa g Lys acc; Thr ttt Phe act Thr ttt Phe ttc; Leu act Thr acc Thr ttc Ph e ca g Gin 150 ccgt Ar g ttg Le u tcc Ser gtc; Val1 cgtg Vali gac Asp c c Ala ttg Leu ccc; Pro cg Ala tct Ser a ca Thr 135 t tg Leu ccc; Pro c;cg Ala att Ile t tg Leu ctc Leu cg t Arc; atc; Met ttc Phe gtt Val1 ttg Leu gcg Al a 120 a cg Thr ccg Pro t cg; Ser ctg Leu aa t Asn 200 Gi y att Ile c99;C Gly ttt Ph e ca a Gin gg t Gly gag Giu 105 acc; TIhr tgc; Trp tgg Trp att Ile cat His 185 ca c Gin ctc; Le u Aia Vali qtc; Val ccc; Pro ttt Phe t ct Ser ValI Gi y ga t Asp cggt Giy 170 act Thr t gc T rp ggt Gly cgtq Vali act Thr cagc Gin 60 tcgc Trp gg t Giy Giy act Thr aagc Lys 140 cc;t Arc; cg Aia att Ile gc9t Gly gtc; Val1 220 tc9c; Trp ttt Phe Giy 45 tcgg Trp Giy Giy Ala ttt Phe 125 aac; Lys tcc; S er acgt Ser att Ile acc Ser 205 tcc; Ser cgct Ala 30 atc; Met c9ct Gly acc Thr ttt Phe gag Glu 110 gct Ala 9ccc Gly cgct Gly acg Thr ttc; Leu 190 atc; Met cgat Asp 15 tcc; Ser aac; Ly s ctc; Leu ga t Asp ggt Gly 95 cgct Ala 9c; c Al a aac; Lys tat T yr aat As n 175 ttc; Leu ttc Phe cat His atc; Met acc; Thr ttc Ph e gag Giu 80 act Th r cgc Ala att Ile act Th r atc Ile 160 ccc; Pro act Th r cca Pro ct t Leu ccc; Pro atc; Met att Ile tcgc Trp gc t Al a atc; Met cgtt Val c c Al a 145 gat Asp tct Ser gt t ValI acc Thr tcc Ser tat Tyr tcgc T rp ttc; Leu ttc Phe cgct Al a tct Ser cgct Gly 130 cgc Ala aac; Lys c c Ala cgc Ala ctc; Val 210 act Thr tcc; Ser c c Al a ctc; Leu 9ccc Gly cgc Ala ttc; Leu 115 cgca Gi y atc; Met ctgc Leu att Ile ct t ValI 195 cagc Gin tac Tyr atc; Met tat Tyr Gi y atc; Met cgtc Val 100 9C~c Gly att Ile cct Pro agct Ser gag Glu 180 c c Ala att Ile act Thr aac; Lys tcc; Ser ctc; Leu atc; Met cgct Gly ttt Ph e att Ile Ala ga t Asp 165 cc t Pro tat Tyr cc; Pro 163 211 259 307 355 403 451 499 547 595 643 691 739 787 ttc; ttt cgc atc; tca ttt Leu Phe Ala Met Ser Phe 215 ctc cgcc att ctc; Val Gly Ilie Val cgcc atc; cgca att atc; Gly Met Gly Ilie Met 225 WO 01/00805 WO 0100805PCTIBOOIOO926 cgt ttg Arg Leu 230 gtg tcg Val Ser att gct Ilie Ala ttt gtt Phe Val.

cct cgt Pro Arg 295 ggt tgg Gly Trp 310 gtg gat Val Asp tat atc Tyr Ilie atc gcg Ilie Ala att gtt Ilie Val 375 ccq gag ttg aag aag cct gag tat ttg Leu ggc Gly cct Pro ttg Leu 280 ttc Ph e g ct Al a ccg Pro ggt Gi y gtg ValI 360 gcg Al a cg c Lys gcg Ala gct Al a 265 gg C Gly ttt Phe act Thr aag Lys ttt Ph e 345 ctc Leu atc Ile Lys gcg Al a 250 g cg Ala ac Th r 99C Gi y g cc Ala t tg Leu 330 gct Ala gca Al a gtg Val Pro 235 a cg Thr att Ile atc Ile gag Glu gcg Al a 315 aag Lys cca Pro ggC Gly att Ile Giu gat Asp g ct Ala aac Asn aag Ly s 300 gt t Val1 t cg Ser ttt Phe ttt Phe ttt Phe 380 Tyr ta c Tyr ga t Asp tgt Cys 285 tgg Trp gct Ala gg a Gly gaa Glu acc Th r 365 ggc Gly Leu ct C Leu tac Tyr 270 tgt Cys ct g Leo acc Th r g cg Ala atc Ile 350 atg Met ct C Leo gat Asp att Ilie 255 tgg Trp ttc Phe gag Glu ggt Gly ctt Leo 335 gg9c Gly 9gg Gly g cg Ala Arg 240 gcg Ala gt t Val1 ttc Phe cg c Arg atc Ile 320 aat Asn atg Met ttg Leo tgg *Trp Asp ttt Phe cct Pro ttt Ph e g cg Ala 305 g CgC Ala gag Glu acc Thr ggt Gly ggt Gly 385 Thr ggt Gly ctt Leo ttc Phe 290 atc Ile t tg Leo tac Tyr atc Ile t gg Trp 370 ctg Leo ValI att Ile gtg ValI 275 t gg Trp ttc Phe ctg Leo ggc Gly atc Ile 355 gcg Ala aag Lys cgc gac acc gtg aat tcc As n gcc Ala 260 gt g Va 1 gtt Val ggt Gly aa g Lys gtg Val1 340 g cg Ala t cg S er t gg Trp Ser 245 tcg S er ctg Leo g cg Ala t gg Trp atc Ile 325 gct Ala c cg Pro t tg Leo ttg Leo 835 883 931 979 1027 1075 1123 1171 1219 1267 1316 1329 Pro Glu Arg gga cat gtc Gly His Val 395 cgc ggc gag ggt aag ccg caa taaaggttgg Arg Gly Giu Gly Lys Pro Gin 400 aagcgccggg tct <210> 508 (211> 402 <212> PRT (213> Corynebacteriom giotarnicom <400> 508 Met Ilie Leo Gly Pro Lys Val Leo Gly Leo Ile Gly Trp Ser Asp His 1 5 10 Leo Ser Thr Tyr Thr Thr Val Leo Ilie Ala Ilie Val Phe Ala Ser Met 25 Pro Tyr Ser Met Lys Phe Asp Arg Gly Val Arg Thr Gly Met Lys Thr 40 WO 01/00805 PCT/IB00/00926 Met Trp Ala Tyr Ser Thr Ala Met Phe Val Gly Gin Trp Gly Leu Phe 55 Ile Leu Leu Gly Leu Phe Leu Phe Gin Pro Val Trp Gly Thr Asp Glu 70 75 Trp Phe Gly Met Met Leu Pro Val Gly Phe Val Gly Gly Phe Gly Thr 90 Ala Ala Ala Val Gly Thr Ala Leu Glu Ser Ser Gly Ala Glu Ala Ala 100 105 110 Met Ser Leu Gly Phe Thr Ser Ala Thr Val Gly Thr Phe Ala Ala Ile 115 120 125 Val Gly Gly Ile Ile Phe Thr Thr Trp Gly Ile Lys Lys Gly Lys Thr 130 135 140 Ala Ala Met Pro Ala Gin Leu Pro Trp Asp Leu Arg Ser Gly Tyr Ile 145 150 155 160 Asp Lys Leu Ser Asp Arg Pro Ser Ile Gly Lys Ala Ser Thr Asn Pro 165 170 175 Ser Ala Ile Glu Pro Leu Ala Leu His Thr Gly Ile Ile Leu Leu Thr 180 185 190 Val Ala Val Ala Tyr Ser Ile Asn Gin Trp Leu Gly Ser Met Phe Pro 195 200 205 Thr Val Gin Ile Pro Leu Phe Ala Met Ser Phe Val Val Gly Ile Val 210 215 220 Gly Met Gly Ile Met Arg Leu Leu Lys Lys Pro Glu Tyr Leu Asp Arg 225 230 235 240 Asp Thr Val Asn Ser Val Ser Gly Ala Ala Thr Asp Tyr Leu Ile Ala 245 250 255 Phe Gly Ile Ala Ser Ile Ala Pro Ala Ala Ile Ala Asp Tyr Trp Val 260 265 270 Pro Leu Val Val Leu Phe Val Leu Gly Thr Ile Asn Cys Cys Phe Phe 275 280 285 Phe Phe Trp Val Ala Pro Arg Phe Phe Gly Glu Lys Trp Leu Glu Arg 290 295 300 Ala Ile Phe Gly Trp Gly Trp Ala Thr Ala Ala Val Ala Thr Gly Ile 305 310 315 320 Ala Leu Leu Lys Ile Val Asp Pro Lys Leu Lys Ser Gly Ala Leu Asn 325 330 335 Glu Tyr Gly Val Ala Tyr Ile Gly Phe Ala Pro Phe Glu Ile Gly Met 340 345 350 Thr Ile Ile Ala Pro Ile Ala Val Leu Ala Gly Phe Thr Met Gly Leu 355 360 365 WO 01/00805 PCTIIBOO/00926 Giy Trp Ala Ser Leu Ilie Val Ala Ile Val Ile Phe Giy Leu Ala Trp 370 375 380 Gly Leu Lys Trp Leu Pro Giu Arg.Gly His Val Arg Gly Giu Gly Lys 385 390 395 400 Pro Gin <210> 509 <211> 1776 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1753) <223> RXA02171 <400> 509 aaccacattc gtcattactt acatctatqt catgtttgcg tcaggctgct attcgccaga agatggaagg ttaatcagat aacaagaact tggagcctcg ctc Leu aac Asn ctt Leu gg t Giy tac Tyr aa C Asn ctt Leu ttc Phe ctt Le u atc Ilie cgc Arg gct Aia ctq Leu ggt Gi y gtt Vai acc Thr gtt Vali gt C Vali acc Thr a cc Th r 60 ttc Phe ctt Leu gtg Val1 Ct 9 Leu tct Ser ttc Phe agc Ser ca 9 Gin ctc Leu tac Tyr gca Aia tcC Ser 125 gag Giu att Ile ga a Giu aa C Asn gga Giy tcC Ser gag Giu 110 ttc Ph e ggC Giy att Ile tcc Ser ggt Giy atc Ile atc Ile cca Pro cga Arg gcg Ala gtc Val1 gtg Vali a cc Th r ctg Leu gtt Val1 80 gg C Giy ctt Leu ctg Leu gtt Vali atg Met 1 ggt Giy a cg Thr gac Asp gct Al a 99 t Giy ttc Phe cgt Arg cg t Arg acc Thr aat Asn atg Met ttc Phe atc Ile 9 ca Aia ttc Phe aac Asn cag Gin 130 ctc Leu ccg Pro acc Th r tac Tyr gca Aia att Ile gtc Val gtg Val 115 aaa Lys ttt Phe att Ile gt g Val1 acc Thr gg t Giy tca Ser gca Aia 100 ggc Giy cca Pro tac Tyr aat tcc act att Asn Ser Thr Ilie ctt Le u gtg Vai ggt Giy ga c Asp ctc Leu tgg Trp cgc Arg gtc Val1 ttg Leu 115 163 211 259 307 355 403 451 499 547 cgc gtc gct gcg gcc Arg Vai Aia Ala Aia tgc ggt acc ctc Cys Gly Thr Leu WO 01/00805 WO 0100805PCTIIBOO/00926 atc Ile 150 atc Ile gtc ValI gtt Val acc Thr aat Asn 230 ggg Gly act Thr ctt Leu cct Pro att Ile 310 g c a Ala gct Ala g cg Al a gqt Gly gct Ala cac His atg Met ca g Gln qtt Val1 215 ga c Asp tac Tyr ct g Leu gg a Gly acc Th r 295 gg t GI y Ctg Leu gct Ala ctg Leu ctt Leu 375 a tg Met ttC Phe gcc Ala 185 att Ile acc Th r gt t ValI cca Pro act Th r 265 gct Ala aag Lys ttc Ph e gg t GI y cc t Pro 345 tcc Ser att Ile gct Ala a aq Lys 170 tac Tyr aag Lys ttC Phe gag Glu acc Th r 250 ca g Gln qqt Gly gaa Glu tac Tyr cca Pro 330 ctg Leu qca Ala acc Thr ggt Gly 155 tgg Trp qt t Val1 g ca Ala gtC Val a ag Lys 235 cag Gin ctg Leu ctg Leu g ca Ala ctg Leu 315 ga c Asp ctg Leu gtt ValI gca Al a g ca Ala ca g Gin ct t Leu g t t ValI aag Ly s 220 ca c His atc Ile ga c Asp cca Pro cgt Arg 300 atg Met cgc Arg qcc Al a gcg Al a t cc Ser 380 ggt Gly gca Ala ctt Leu ctg Leu 205 gt g Val1 gcc Ala ctg Le u ttC Ph e cac His 285 aag Lys a cc Thr gt C Val1 ttC Phe ttC Ph e 365 gct Ala t cg S er gt t ValI Gly 190 ctg Leu tct S er g ct Ala gag Glu att Ile 270 gtt Val tct Ser ctg Leu att Ile gag Giu 350 gct Al a gct Al a ct t Leu gtt ValI 175 gg t Gly gtc Val ggt Gly t ca Se r cc t Pro 255 tcc Ser ct g Le u gtg Val1 gtC Val1 gcc Ala 335 ct t Leu acc Th r gtt Val1 gtg Val1 160 gtC Val1 atg Met ggt G ly ggC Gly ga t Asp 240 ggt Gly ttg Leu atg Met acc Th r ctt Leu 320 g ca Al a gg t Gly gt t ValI gg t Gly t cc Ser gg t GI y aag Lys gg C Gi y ctg Le u 225 ta c Tyr ctg Leu g ct Al a cg c Arg t gg T rp 305 gg t Gi y cca Pro ggt Gly ctc Leu cac His 385 gt t Val1 atc Ile gg c Gly gtt Val1 210 acc Th r gct Ala ca g Gln ctc Le u ttc Phe 290 g ct Al a tac Tyr ggt Gly tcc Ser g cc Ala 370 gac Asp ctg Leu gtt Val acc Thr 195 gcc Ala acc Thr g cc Al a tac Tyr gct Ala 275 tac Tyr atc Ile Gly g ct Ala atc Ile 355 gtg Val1 atc Ile ctg gac Leu Asp 165 ggc att Gly Ile 180 aca tac Thr Tyr att atg Ile Met ctt tta Leu Leu acc aag Thr Lys 245 ggt gca Gly Ala 260 ctg tgt Leu Cys acc gtt Thr Val gtc ctc Val Leu gct gcg Ala Ala 325 gct aat Ala Asn 340 ttc atg Phe Met gtc gca Val Ala tac aac Tyr Asn 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 WO 01/00805 WO 0100805PCTJIBOO/00926 gct Ala 390 t cc Ser att Ile gca Al a tgg Trp Ct C Leu 470 aat Asn aag Lys atc Ile gaa gt t ValI cg C Arg ctt Le u gtt Val1 aag Lys 455 atc Ile ga C Asp aac As n ggC Gi y atg atc Ilie atc Ile g ca Ala gca Ala 440 aag Lys tcc Ser aq C Ser cca Pro act Thr 520 gaa cgc aac ggt Arg Asn Gly 395 acc gtt gtc Thr Val Val 410 atg acc cag Met Thr Gin 425 gca tcc gct Ala Ser Ala ttc aac acc Phe Asn Thr gcg ctq ctg Ala Leu Leu 475 gca atg gtt Ala Met Val 490 qgc ctc gtc Gly Leu Val 505 ttg gtt ggc Leu Vai Gly gtt cgt tcc cag tcc acc Gin Ser Thr gaa qcg Giu Ala 400 gag cag gtc cga gta Giu Gin Val Arg Vai gtC Val1 aac Asn aac Asn acc Th r 460 ctg Leu cca Pro tcc Ser aag Ly s ct C att Ile gtt Val1 ctg Leu 445 99c Gly atc Ile ggt Gly atc Ilie c ca Pro 525 acc ggc ctg att.

Gly Leu Ilie 415 gcg ttc ctc Ala Phe Leu 430 cca acc atc Pro Thr Ile gct gtg gcc Ala Val Ala ttc ctg tcc Phe Leu Ser 480 gca gac tgg Ala Asp Trp 495 cca ctg gca Pro Leu Ala 510 gac aac atg Asp Asn Met ggt gtc ggt Gly Val Gly tcC Ser gtg Val1 ctg Leu gct Ala 465 cca Pro g ca Al a ttc Phe ga t Asp att Ile gCC Ala tac Tyr 450 atc Ile g ca Al a atc Ilie atC Ile gat Asp 530 405 99a Gly ttc Phe tac Tyr gg t Gly gg t Gi y 485 ct g Le u tgg Trp gcc Ala 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1776 Giu Met Giu Val Arg Ser Leu Thr 535 540 gtt gaa aag gct gtt Val Giu Lys Ala Val 545 gat cac taaatctagt ttctgaagtt att.

Asp His 550 <210> 510 <211> 551 <212> PRT <213> Corynebacteriun giutarnicum <400> 510 Met Asn Ser Thr Ilie Leu Leu Ala Gin Asp Ala Val Ser Giu Giy Vai 1 5 10 Gly Asn Pro Ilie Leu Asn Ilie Ser Val Phe Val Val Phe Ilie Ile Val 25 Thr Met Thr Val Val Leu Arg Val Gly Lys Ser Thr Ser Glu Ser Thr 40 WO 01/00805 PCT/IB00/00926 Asp Phe Tyr Thr Gly Gly Ala Ser Phe Ser Gly Thr Gin Asn Gly Leu 55 Ala Ile Ala Gly Asp Tyr Leu Ser Ala Ala Ser Phe Leu Gly Ile Val 70 75 Gly Ala Ile Ser Leu Asn Gly Tyr Asp Gly Phe Leu Tyr Ser Ile Gly 90 Phe Phe Val Ala Trp Leu Val Ala Leu Leu Leu Val Ala Glu Pro Leu 100 105 110 Arg Asn Val Gly Arg Phe Thr Met Ala Asp Val Leu Ser Phe Arg Leu 115 120 125 Arg Gin Lys Pro Val Arg Val Ala Ala Ala Cys Gly Thr Leu Ala Val 130 135 140 Thr Leu Phe Tyr Leu Ile Ala Gin Met Ala Gly Ala Gly Ser Leu Val 145 150 155 160 Ser Val Leu Leu Asp Ile His Glu Phe Lys Trp Gin Ala Val Val Val 165 170 175 Gly Ile Val Gly Ile Val Met Ile Ala Tyr Val Leu Leu Gly Gly Met 180 185 190 Lys Gly Thr Thr Tyr Val Gin Met Ile Lys Ala Val Leu Leu Val Gly 195 200 205 Gly Val Ala Ile Met Thr Val Leu Thr Phe Val Lys Val Ser Gly Gly 210 215 220 Leu Thr Thr Leu Leu Asn Asp Ala Val Glu Lys His Ala Ala Ser Asp 225 230 235 240 Tyr Ala Ala Thr Lys Gly Tyr Asp Pro Thr Gin Ile Leu Glu Pro Gly 245 250 255 Leu Gin Tyr Gly Ala Thr Leu Thr Thr Gin Leu Asp Phe Ile Ser Leu 260 265 270 Ala Leu Ala Leu Cys Leu Gly Thr Ala Gly Leu Pro His Val Leu Met 275 280 285 Arg Phe Tyr Thr Val Pro Thr Ala Lys Glu Ala Arg Lys Ser Val Thr 290 295 300 Trp Ala Ile Val Leu Ile Gly Ala Phe Tyr Leu Met Thr Leu Val Leu 305 310 315 320 Gly Tyr Gly Ala Ala Ala Leu Val Gly Pro Asp Arg Val Ile Ala Ala 325 330 335 Pro Gly Ala Ala Asn Ala Ala Ala Pro Leu Leu Ala Phe Glu Leu Gly 340 345 350 Gly Ser Ile Phe Met Ala Leu Ile Ser Ala Val Ala Phe Ala Thr Val 355 360 365 Leu Ala Val Val Ala Gly Leu Ala Ile Thr Ala Ser Ala Ala Val Gly WO 01/00805 WO 0100805PCTIIBOO/00926 370 375 380 His Asp Ilie Tyr Asn Ala Val Ilie Arg Asn Gly Gin Ser Thr Giu Ala 385 390 395 400 Glu Gin Val Arg Val Ser Arg Ile Thr Val Val Val Ile Gly Leu Ile 405 410 415 Ser Ilie Vai Leu Gly Ilie Leu Ala Met Thr Gin Asn Val Ala Phe Leu 420 425 430 Val Ala Leu Ala Phe Ala Val Ala Ala Ser Ala Asn Leu Pro Thr Ile 435 440 445 Leu Tyr Ser Leu Tyr Trp Lys Lys Phe Asn Thr Thr Giy Ala Val Ala 450 455 460 Ala Ilie Tyr Thr Gly Leu Ilie Ser Ala Leu Leu Leu Ile Phe Leu Ser 465 470 475 480 Pro Ala Val Ser Gly Asn Asp Ser Ala Met Val Pro Gly Ala Asp Trp 485 490 495 Ala Ile Phe Pro Leu Lys Asn Pro Gly Leu Val Ser Ilie Pro Leu Ala 500 505 510 Phe Ilie Ala Gly Trp Ilie Gly Thr Leu Val Gly Lys Pro Asp Asn Met 515 520 525 Asp Asp Leu Ala Ala Giu Met Giu Val Arg Ser Leu Thr Gly Val Gly 530 535 540 Val Giu Lys Ala Val Asp His 545 550 <210> 511 <211> 1333 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(1333) <223> RXA00902 <400> 511 ttgttattgg tttgttcact cccatgcaat gtacgatcgg ttctaacgtc tacggcaatt gacacgtcaa tttaattcaq gaaaggqgtg gggagaattt atg tct agt ggt ttt 115 Met Ser Ser Gly Phe 1 gaa tat gtt caa cat cct cga cga qcc ctc cct ccg cct ata cct gaa 163 Glu Tyr Val Gin His Pro Arg Arg Ala Leu Pro Pro Pro Ile Pro Glu 15 cgg aaa ggt cct gcc gca gca ttc ctg ccg gga act ttc cat ccc att 211 Arg Lys Gly Pro Ala Ala Ala Phe Leu Pro Gly Thr Phe His Pro Ile 30 WO 01/00805 WO 0100805PCTA~BOO/00926 aat cca aag aat att gca gcg agc cac qat cag gtg ctt cta tct 9qt 259 Asn Pro Lys Asn Ile Ala Ala Ser His Asp Gin Val Leu Let' Ser Gly 45 tgg ggc aaa ttt gtg cgt tgg ctc ttg gta ttg ttg tcc att ttg gtc 307 Trp Gly Lys Phe Val Arg Trp Let' Leu Val Leu Leu Ser Ile Let' Val 60 atc atc att gqc atc aac ctc atc ttg gac ggt gtc tac gga ttt ggt 355 Ilie Ile Ilie Gly Ile Asn Leu Ile Leu Asp Gly Val Tyr Gly Phe Gly 75 80 act ttt tca acc acc cag atg tac caa gtt gcg aaa gat cca ctc att 403 Thr Phe. Ser Thr Thr Gin Met Tyr Gin Val Ala Lys Asp Pro Let' Ilie 95 100 ggt gtg ctg atc ggt atc ttg gct acg gcc ttg gtg caa tca tca acc 451 Giy Val Le' Ilie Gly Ile Let' Ala Thr Ala Leu Val Gin Ser Ser Thr 105 110 115 acc acc aca acg ttg acg gtg act .gca gtt ggt acg ggc att gtg tcg 499 Thr Thr Thr Thr Let' Thr Val Thr Ala Val Gly Thr Gly Ilie Val Ser 120 125 130 gtg cct gtg gcg att ccg atc att ctt ggc gca aat atc ggt acg acg 547 Val Pro Val Ala Ilie Pro Ile Ile Let' Gly Ala Asn Ile Gly Thr Thr 135 140 145 atc acc gcg atg ctc gtt gcg ttt tct tat gtg ggt gaa cgc agg gag 595 Ilie Thr Ala Met Let' Val Ala Phe Ser Tyr Val Gly Git' Arg Arg Git' 150 155 160 165 ttt aag cga qct ttt acq gtt gcc gcc atg cat gtg tgg ttt aac gtg 643 Phe Lys Arg Ala Phe Thr Val Ala Ala Met His Val Trp Phe Asn Val 170 175 180 ctc gtc att ctt gtt cta ttt gtt gtg gaa ttg ctc ttt cat cca ttc 691 Let' Val Ilie Let' Val Let' Phe Val Val Git' Let' Let' Phe His Pro Phe 185 190 195 cgc aca att agt ggt gcg atc gca acg gag atc aca ctg aca act ggt 739 Arg Thr Ilie Ser Gly Ala Ilie Ala Thr Gi' Ile Thr Let' Thr Thr Gly 200 205 210 ggc tct ttg cct acc agt gqt gtg atg acc aag att ttt gat ccc cca 787 Gly Ser Let' Pro Thr Ser Gly Val Met Thr Lys Ile Phe Asp Pro Pro 215 220 225 acc caa ctt ctg ggt atg aat ggt ctt atc ggt tcg atc ggc aat cct 835 Thr Gin Let' Let' Gly Met Asn Gly Let' Ile Gly Ser Ile Gly Asn Pro 230 235 240 245 agt att tcg gcg att gta tgt ctt gtg gtg ggc acc att ctt att ctg 883 Ser Ilie Ser Ala Ilie Val Cys Let' Val Val Gly Thr Ile Let' Ile Let' 250 255 260 att tcg qtg cgt gcc atg agt tct cag atc cga acc att acg gca gcg 931 Ilie Ser Val Arg Ala Met Ser Ser Gin Ilie Arg Thr Ile Thr Ala Ala 265 270 275 acc gta acc tca att atg gac aag gtg atc aat cca gag aac agc ccc 979 WO 01/00805 PCTIBOO/00926 Thr Val Thr Ser Ile Met Asp Lys Val Ile Asn Pro Giu Asn Ser Pro 280 285 290 aag gcg acg att ctt tcc aat ttc tgg agc ttc att ctt gga gtt ttg 1027 Lys Ala Thr Ile Leu Ser Asn Phe Trp Ser Phe Ile Leu Gly Val Leu 295 300 305 ttc acg ctc atg gtc act gcc tcg tca gtg acc gtg gct tcc atg cag 1075 Phe Thr Leu Met Val Thr Ala Ser Ser Val Thr Val Ala Ser Met Gin 310 315 320 325 cca gtg gct gcc tct ggt gtc gtt aag caa aag cca ttg ctg ggc qtc 1123 Pro Vai Ala Ala Ser Gly Val Val Lys Gin Lys Pro Leu Leu Giy Val 330 335 340 att ttg ggt gcc aac gtg ggc acc acg gtg acc gca atg ttt gct act 1171 Ile Leu Gly Ala Asn Vai Giy Thr Thr Val Thr Ala Met Phe Ala Thr 345 350 355 ttc gcg att gtc agc gat cag ggt gag ttc qct att cag gct gcg ttg 1219 Phe Ala Ile Val Ser Asp Gin Gly Giu Phe Ala Ile Gin Ala Ala Leu 360 365 370 atc cac ctc att gtg aac ttc acc ggc gca tta cta gtg ctg tgt att 1267 Ile His Leu Ile Val Asn Phe Thr Gly Ala Leu Leu Val Leu Cys Ile 375 380 385 ccg cag ctt gcc aat gtg att att cac ttg gcc gag aaa act gcg aac 1315 Pro Gin Leu Ala Asn Val Ile Ile His Leu Ala Giu Lys Thr Ala Asn 390 395. 400 405 ctc act gcc cgc agt tac 1333 Leu Thr Ala Arg Ser Tyr 410 <210> 512 <211> 411 <212> PRT <213> Corynebacterium giutamicun <400> 512 Met Ser Ser Gly Phe Giu Tyr Val Gin His Pro Arg Arg Ala Leu Pro 1 5 10 Pro Pro Ile Pro Giu Arg Lys Gly Pro Ala Ala Ala Phe Leu Pro Gly 25 Thr Phe His Pro Ile Asn Pro Lys Asn Ile Ala Ala Ser His Asp Gin 40 Val Leu Leu Ser Gly Trp Gly Lys Phe Val Arg Trp Leu Leu Val Leu 55 Leu Ser Ilie Leu Val Ilie Ile Ile Gly Ile Asn Leu Ile Leu Asp Gly 70 75 Val Tyr Gly Phe Gly Thr Phe Ser Thr Thr Gin Met Tyr Gin Val Ala 90 Lys Asp Pro Leu Ile Gly Val Leu Ile Gly Ile Leu Ala Thr Ala Leu WO 01/00805 PCT/IB00/00926 100 105 110 Val Gin Ser Ser Thr Thr Thr Thr Thr Leu Thr Val Thr Ala Val Gly 115 120 125 Thr Gly Ile Val Ser Val Pro Val Ala Ile Pro Ile Ile Leu Gly Ala 130 135 140 Asn Ile Gly Thr Thr Ile Thr Ala Met Leu Val Ala Phe Ser Tyr Val 145 150 155 160 Gly Glu Arg Arg Glu Phe Lys Arg Ala Phe Thr Val Ala Ala Met His 165 170 175 Val Trp Phe Asn Val Leu Val Ile Leu Val Leu Phe Val Val Glu Leu 180 185 190 Leu Phe His Pro Phe Arg Thr Ile Ser Gly Ala Ile Ala Thr Glu Ile 195 200 205 Thr Leu Thr Thr Gly Gly Ser Leu Pro Thr Ser Gly Val Met Thr Lys 210 215 220 Ile Phe Asp Pro Pro Thr Gin Leu Leu Gly Met Asn Gly Leu Ile Gly 225 230 235 240 Ser Ile Gly Asn Pro Ser Ile Ser Ala Ile Val Cys Leu Val Val Gly 245 250 255 Thr Ile Leu Ile Leu Ile Ser Val Arg Ala Met Ser Ser Gin Ile Arg 260 265 270 Thr Ile Thr Ala Ala Thr Val Thr Ser Ile Met Asp Lys Val Ile Asn 275 280 285 Pro Glu Asn Ser Pro Lys Ala Thr Ile Leu Ser Asn Phe Trp Ser Phe 290 295 300 Ile Leu Gly Val Leu Phe Thr Leu Met Val Thr Ala Ser Ser Val Thr 305 310 315 320 Val Ala Ser Met Gin Pro Val Ala Ala Ser Gly Val Val Lys Gin Lys 325 330 335 Pro Leu Leu Gly Val Ile Leu Gly Ala Asn Val Gly Thr Thr Val Thr 340 345 350 Ala Met Phe Ala Thr Phe Ala Ile Val Ser Asp Gin Gly Glu Phe Ala 355 360 365 Ile Gin Ala Ala Leu Ile His Leu Ile Val Asn Phe Thr Gly Ala Leu 370 375 380 Leu Val Leu Cys Ile Pro Gin Leu Ala Asn Val Ile Ile His Leu Ala 385 390 395 400 Glu Lys Thr Ala Asn Leu Thr Ala Arg Ser Tyr 405 410 <210> 513 WO 01/00805 <211> 1440 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1417) <223> RXA00941 <400> 513 agccgqqqtg gccgtttgcg tgtacgggtt ttgcttatcg PCTJIBOO/00926 acgcccactt ctgattttaa aggcaaatat tttgtgtcac agcgagtaat cttttcgqqc atg aac cta acc cgg Met Asn Leu Thr aa t Asn ctt Le u ct 9 Leu cct Pro atc Ile caa Gin t cg Ser gtt Val1 tcC S er gag Glu 150 ttt Phe ggc ga c Asp ggc Gly atc Ile act Th r gg C Gly gt t Val gcq Al a gga GI y gag Glu 135 gcg Ala gtC Val1 tgt agg Arg 9gc Giy tct S er gaa Giu gct Ala ttt Phe ct t Leu ctt Leu 120 ttt Phe ttt Phe gtg ValI gtc ctc Leu tca Se r ttC Ph e act Thr tcg Ser qcg Ala tca S er 105 9gC Gly tct Ser tgg Trp gca Al a cc t ga t Asp gg t Gly gtc Val1 t cc S er ctg Leu t tg Leu gtg Val ctg Leu c Ca Pro g cg Ala gga Gly 170 gca cga Arg atc Ile a tg Met ctq Leu qg C Gly 75 tct Ser t cg Ser GI y cg a Ar g ctg Leu 155 t CC Ser att ctg Leo Gly gct Ala ct C Leu 60 ggt Gly ttg Leu ctg Leu gct Al a aaa Lys 140 gg C Gly ga t Asp tac ccc Pro tgg Trp g cg Al a 45 gga Gly ttg Leu cta Leu g ca Al a gaa Glu 125 gtt ValI t gg Trp aat Asn gc9 gta ValI g ca Al a 30 ttg Leu t cc Ser ct g Le u gtt Val1 atg Met 110 ctc Leu cg t Arg atc I le gg t Gly gtg act Thr 15 ttg Leu gcc Ala atc Ile gc9 Ala tat Tyr 95 ttg Leu ccc Pro ggc Gly a tg Met tgg Trp 175 tat tcc Ser ga t Asp act Thr ggt Gly ga c Asp 80 9gc Gly atg Met gtt ValI cgc Arg g Ct Al a 160 cg t Arg gtc 1 aaa Lys gcc Al a cat His ttc Phe aag Lys gtt Vali gcg Al a gca Ala atg Met 145 gca Ala t gg Trp cg t cat His a tg Met t gg Trp gtt Val1 ttg Leu gcc Ala ctg Leo t cc Ser 130 gtc Val1 atc Ile gcg Ala ct c aaa Ly s ga t Asp gg C Gly ggc Gi y Gly act Thr cg c Arg 115 act Thr gt t Val1 gtC Val1 tta Leo gg a aag Lys gtc Val cta Leo atg Met cgt Arg gqc Gly 100 ttc Phe ctg Leu atc Ile gga Gly g Ct Al a 180 ctt Arg att Ile gga Gly t cc Ser gcc Al a cga Arg gcg Ala gt c Val att Ile ct g Leo acc Thr 165 ctt Leo cca 115 163 211 259 307 355 403 451 499 547 595 643 691 WO 01/00805 PCTIBOOIOO926 Gly Cys Val Pro Ala Ile Tyr Ala Val Tyr Val Arg Leu Gly Leu Pro 185 190 195 gaa tca gta cgt ttc ctg gag aag aaa ggc cgg cac gac gaa gca gaa 739 Glu Ser Val Arg Phe Leu Giu. Lys Lys Gly Arg His Asp Giu Ala Glu 200 205 210 gcc atc gtt gtt tcc ttc gaa gaa gct gcc gct gcc gaa ggt aaa gct 787 Ala Ile Val Val Ser Phe Giu Glu Ala Ala Ala Ala Glu Gly Lys Ala 215 220 225 gcc gat gcc acc acc gct gtg gtt cat gac aac gct gcc gag ggt tcc 835 Ala Asp Ala Thr Thr Ala Val Val His Asp Asn Ala Ala Glu Gly Ser 230 235 240 245 gta tcc atc tgq tca gct gct ttg cgc aag cgc acc gtc gcg ctg tgg 883 Val Ser Ile Trp Ser Ala Ala Leu Arg Lys Arg Thr Val Ala Leu Trp 250 255 260 atc gtg tgg ttc tgc atc aac ttg tcc tac tac gga gcc ttc att tgg 931 Ile Val Trp Phe Cys Ile Asn Leu Ser Tyr Tyr Gly Ala Phe Ile Trp 265 270 275 att cca tcg ctg ctg gtt gcc gac ggt ttc acc ctc gtg aag tct ttc 979 Ile Pro Ser Leu Leu Val Ala Asp Gly Phe Thr Leu Val Lys Ser Phe 280 285 290 caa ttc act ctg atc atc acc ttg gct cag ctt cca ggc tat gcg gtt 1027 Gin Phe Thr Leu Ilie Ilie Thr Leu Ala Gin Leu Pro Gly Tyr Ala Val 295 300 305 gca gcg tqg ttg att gaa aaq tgg ggc cgt cgc agc aca ttg gcc acg 1075 Ala Ala Trp Leu Ilie Giu Lys Trp Giy Arg Arg Ser Thr Leu Ala Thr 310 315 320 325 ttc ctg gtt ggt tct gct atc tct gca gcg ctc tac ggc ttg gca aat 1123 Phe Leu Vai Gly Ser Ala Ile Ser Ala Ala Leu Tyr Gly Leu Ala Asn 330 335 340 gtg gag tgg cag atc ctg gtc gca ggt tgt tta cta tcc ttc ttc aac 1171 Val Giu Trp Gin Ilie Leu Val Ala Gly Cys Leu Leu Ser Phe Phe Asn 345 350 355 ctg ggc gca tgg ggc gca ctq tat gcc att ggg ccg gag ctt tat ccc 1219 Leu Gly Ala Trp Gly Ala Leu Tyr Ala Ilie Gly Pro Glu Leu Tyr Pro 360 365 370 act aat gtc cgt gga act gga acg ggt gct gcc gcg gga ttc ggg cgc 1267 Thr Asn Val Arg Gly Thr Gly Thr Giy Ala Ala Ala Gly Phe Gly Arg 375 380 ,385 att gct tcc atc atc gct ccg ctc att gtt ccg cca gtg att gct ttt 1315 Ilie Ala Ser Ilie Ilie Ala Pro Leu Ilie Val Pro Pro Val Ilie Ala Phe 390 395 400 405 ggt gga cca att gct ttg ttc gct ctc ttt gcc acc gcg ttt gcg atc 1363 Gly Giy Pro Ilie Ala Leu Phe Ala Leu Phe Ala Thr Ala Phe Ala Ilie 410 415 420 gca gcg att gcg gcg ttt acg ctg cct gag cag aag ggt aag tct ctc 1411 Ala Ala Ilie Ala Ala Phe Thr Leu Pro Giu Gin Lys Gly Lys Ser Leu WO 01100805 WO 0100805PCT/EBOOIOO926 425 430 435 gct gat tagtgagatc cttccaccag ttt 1440 Ala Asp <210> 514 <211> 439 <212> PRT <213> Corynebacterium glutamicum <400> 514 Met Asn Leu Thr Arg Asn Asp Arg Leu Asp Arg Leu Pro Val Thr Ser 1 5 10 Lys His Lys Lys Ile Leu Gly Gly Ser Gly Ile Gly Trp Ala Leu Asp 25 Ala Met Asp Val Gly Leu Ilie Ser Phe Val Met Ala Ala Leu Ala Thr 40 His Trp Gly Leu Ser Pro Thr Glu Thr Ser Leu Leu Gly Ser Ile Gly 55 Phe Val Gly Met Ala Ilie Gly Ala Ser Leu Gly Gly Leu Leu Ala Asp 70 75 Lys Leu Gly Arg Arq Gin Val Phe Ala Leu Ser Leu Leu Val Tyr Gly 90 Val Ala Thr Gly Ala Ser Ala Leu Ser Val Ser.Leu Ala Met Leu Met 100 105 110 Ala Leu Arg Phe Val Val.Gly Leu Gly Leu Gly Ala Glu Leu Pro Val 115 120 125 Ala Ser Thr Leu Ilie Ser Glu Phe Ser Pro Arg Lys Val Arg Gly Arg 130 135 140 Met Val Val Ilie Leu Giu Ala Phe Trp Ala Leu Gly Trp Ile Met Ala 145 150 155 160 Ala Ilie Val Gly Thr Phe Val Val Ala Gly Ser Asp Asn Gly Trp Arg 165 170 .175 Trp Ala Leu Ala Leu Gly Cys Val Pro Ala Ilie Tyr Ala Val Tyr Val 180 185 190 Arq Leu Gly Leu Pro Glu Ser Val Arg Phe Leu Glu Lys Lys Gly Arg 195 200 205 His Asp Giu Ala Glu Ala Ilie Val Val Ser Phe Glu Giu Ala Ala Ala 210 215 220 Ala Glu Gly Lys Ala Ala Asp Ala Thr Thr Ala Val Val His Asp Asn 225 230 235 240 Ala Ala Giu Gly Ser Val Ser Ile Trp Ser Ala Ala Leu Arg Lys Arg 245 250 255 WO 01/00805 PCTIBOO/00926 Trp Ile Val Trp Phe Cys Ile Asn Leu Ser Tyr Tyr Thr Gly Leu Pro 305 Ser Tyr Leu Pro Ala 385 Pro Th r Lys Ala Phe 275 Lys Tyr Leu Leu Ph e 355 Leu Ph e Ile Phe Lys 435 Leu 260 Ile Ser Al a Al a Al a 340 Phe Tyr Gly Al a Al a 420 Ser Pro Ph e 295 Ala Leu Glu Gly As n 375 Ala Gly Al a Asp 265 Leu Leu Leu Gly Gin 345 T rp Arg Ile Ile Ala 425 270 Leu Ile Ile Ser 330 Ile Giy Gly Ile Ala 410 Al a ValI Ile Glu' 315 Al a Le u Ala Th r Al a 395 Leu Ph e Giy Ala Gly Ala Gly 350 Ala Gly Ile Le u Pro 430 Thr Leu A r c 320 Le u Leu Gi y Ala Pro 400 Al a Gin <210> 515 <211> 1704 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (101)..(1681) <223> RXN00449 <400> 515 tcgttggctt acatggtcat tgatgaccgg gctgaatatg tgagaaaatc catcccttct ttaaqcaagg qagtgaatta cagaaaagga ttgttcagca atg agc aca cct gac Met Ser Thr Pro Asp 1 att aaa gaa ggc tcg gca gaa tca ccg ggc gaa gta atg gtc gtt gga Ile Lys Glu Gly Ser Ala Giu Ser Pro Gly Giu Val Met Val Vai Gly 15 gac agg cga gag tgq cgt cga caa gca acc ggc atc att gcc ggc ctc Asp Arg Arg Glu Trp Arg Arg Gin Aia Thr Giy Ile Ile Ala Gly Leu 30 WO 01/00805 WO 0100805PCTIIBOO/00926 gt c Val1 acc Thr aac As n tgg Trp gtt Val1 Ccqg Pro g ca Ala gtc Val1 150 atg Met g cc Ala gag Glu atg Met att Ile 230 cat His att Ile qt c Val agt Ser act Th r 75 atc Ile ttc Phe acg Th r aaa Lys gqt Gly 155 ttt Ph e ccg Pro atg Met tat Tyr aat Asn 235 gga Gi y acc Thr tat Tyr gqc Gly gct Ala cca Pro cag Gin a gc S er t gg Trp 140 act Thr t tg Leu atc Ile aaa Lys tct Ser 220 gcc Al a ttt Phe atc Ile t cg S er act Thr ttg Leu g ca Ala ggg Gly 9gc Gly cgt Arg 145 ttg Leu tct S er ctq Leu ttc Phe tca.

Ser 225 tat Tyr att.

Ile gtg Val1 aac Asn gaa Glu atg Met ctt Le u aag Lys gig C Gly 130 atg Met gtc Val1 aa c Asn gca Ala gcc Al a 210 ctc Leu atg Met ctt Le u t tg Leu t cc Se r tac Tyr g ca Ala atc Ile g ca Al a 115 ttt Phe gct Al a ttg Le u act Th r ctg Le u 195 act Th r Gi y t ct Ser 9gt Gly acc Th r 275 gaa Glu aac Asn t gg Trp t tg Le u gct Ala atg Met g cg Ala ttt Ph e 165 acg Th r g ct Al a ctc Le u tta Leu t cg Ser 245 cca Pro gtg Val1 ttc Ph e cg t Arg ggt Gly 310 act Thr ggc GI y acc Thr ga t Asp acc Thr 390 ga t Asp t tg Leu ctg Leu gg C Gly aCe Thr 470 ttc Phe t gg Trp WO 01/00805 aag cca gaa Lys Pro Giu 280 gaa atc gct Glu Ile Ala 295 gtt att ttt Val Ilie Phe cta gat tgg Leu Asp Trp atc gct gcc Ilie Ala Ala 345 ggc gaa cgc Gly Giu Arg 360 gtt ctc ttg Val Leu Leu 375 agc acg gga Ser Thr Gly gcc ctt cca Ala Leu Pro ttc ctg acc Phe Leu Thr 425 eca atc atg Pro Ile Met 440 gag eag aat Giu Gin Asn 455 tgt gcg ttc Cys Ala Phe gge tee ggc Gly Ser Gly etg aac atc Leu Asn Ilie PCTIBOO/00926 a tg Met gaa Glu g eq Al a a ce Th r 330 Giy att Ile Ct t Leu ett Le u ate Ile 410 gag Glu gg t Gly gtt ValI atg Met tac T yr 490 ate Ile aaa Lys atg Met gca Ala 315 qg t Gi y et g Leu ctt Le u ttt Ph e tcc Ser 395 tte Ph e ttc Phe gge Gly ctg Leu ctt Leu 475 att Ile gca Al a ga a Glu ggg Gly 300 get Al a tcc Ser ctg Le u ga t Asp ggt Gly 380 cta L eu att Ile acc Thr gtc Val1 ct g Le u 460 eca Pro aag Lys gtc ValI atc Ile 285 ccg Pro g ca Ala ca g Gin atg Met t gg T rp 365 gge Giy tgg T rp etc Leu tee Ser gCC Ala 445 ctg Leu g tg Val1 ate Ilie atc Ile cet ggc Pro Gly tgg act Trp Thr ctg get Leu Ala ctc tet Leu Ser 335 ttt atc Phe Ilie 350 egt act Arg Thr ggg ctt Gly Leu atc ggt Ile Giy atc ttc Ilie Phe 415 aac acc Asn Thr 430 gta ggt Val Gly acc atc Thr Ilie gca acg Ala Thr g gaa Gly Giu 495 etc att Leu Ilie 510 atg ctt gga Gi y gca Al a tgg Trp 320 ate Ile gtt ValI g ca Al a tca Ser ga a Giu 400 g cc Ala qca Ala atc Ile cca Pro cct Pro 480 atg Met a cg Thr eq t Arg cct Pro 305 gtc Val1 aat Asn ccc Pro gge Gly ctt Le u 385 eta Le u att Ile a ca Thr gga Gly gtc ValI 465 eca Pro gte Val1 att ga a Giu 290 eag Gin tte Phe gac Asp get Al a gaa Giu 370 t et S er get Ala get Ala 9g Al a ctg Le u 450 g ea Al a aae Asn aag Lys ttc ct g Le u gte Val att Ilie tee Ser aae As n 355 ett Leu geg Ala aag Lys gte ValI gea Ala 435 ace Thr etg Leu g eq Ala gg t Gi y ace ate Ile aca Thr cca Pro etc Leu 340 ttt Ph e eca Pro atg Met gga Gly Ct g Leu 420 ace Thr gea Ala tee S er att Ile gg t Gly 500 tac aaa Lys gtg Val1 tta Leu 325 att Ile aaa Lys t gg T rp ttt Phe ct t Le u 405 gt g ValI tte Ph e gy t Gly gea Al a gea Ala 485 Ct g Le u ttc 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1701 Ile Phe Thr Tyr Phe 505 gta gcg ate eca etc ttt ggc ate taaaagttaa eaggceegea WO 01/00805 PCTIBOO/00926 Val Ala Ilie Pro Leu Phe Gly Ile Met Leu 520 525 gtc 1704 <210> 516 <211> 527 <212> PRT <213> Corynebacteriui giutarnicum <400> 516 Met Ser Thr Pro Asp Ilie Lys Glu Gly Ser Ala Giu Ser Pro Gly Giu 1 5 10 Val Met Val Val Gly Asp Arg Arg Giu Trp Arq Arg Gin Ala Thr Gly 25 Ile Ile Ala Gly Leu Vai Leu Aia Ala Leu Val Tyr Leu Leu Phe Pro 40 Ser Asn Ser Val Giu Thr Val Met Gin Ser Ser Gly Val Asp Pro Giu 55 Thr Giu Tyr Thr Asn Asn Ala Met Arg Leu Thr Ala Ala Val Thr Ile 70 75 Leu Met Ala Val Trp Trp Met Thr Giu Ala Ile Pro Leu Ala Ala Thr 90 Ala Leu Ilie Pro Leu Val Ala Phe Pro Ala Phe Gin Val Val Asp Phe 100 105 110 Gly Lys Ala Thr Ala Pro Tyr Aia Asn Pro Thr Ser Phe Leu Phe Leu 115 120 125 Gly Gly Phe Leu Met Ala Leu Gly Leu Gin Lys Trp Asn Leu His Arg 130 135 140 Arg Met Ala Leu Ala Val Val Leu Ala Val Gly Thr Lys Pro Lys Gin 145 150 155 160 Leu Val Leu Gly Phe Met Val Ala Thr Gly Phe Leu Ser Met Trp Val 165 170 175 Ser Asn Thr Ala Thr Ala Val Val Met Leu Pro Ilie Gly Met Ser Val 180 185 190 Leu Ala Leu Thr Ala Giu Thr Val Gly Gly Met Lys Asn Gin Lys Lys 195 200 205 Phe Ala Thr Gly Leu Met Leu Ser Ilie Aia Tyr Ser Ala Ser Ile Gly 210 215 220 Ser Leu Gly Thr Leu Ile Gly Thr Pro Pro Asn Ala Leu Leu Ala Ala 225 230 235 240 Tyr Met Ser Giu Ser His Asp ile His Ile Gly Phe Gly Gin Trp Met 245 250. 255 Ile Leu Gly Val Pro Ile Ala Val Val Phe Thr Ie Ile Ala Trp Leu WO 01/00805 WO 0100805PCTIIBOO/00926 260 265 270 Val Leu Thr Thr Val Phe Lys Pro Glu Met Lys Glu Ile Pro Gly Gly 275 280 285 Arq Giu Leu Ilie Lys Arg Giu Ilie Ala Giu Met Gly Pro Trp Thr Ala 290 295 300 Pro Gin Val Thr Val Gly Val Ilie Phe Ala Ala Ala Ala Leu Ala Trp 305 310 315 320 Val Phe Ile Pro Leu Thr Leu Asp Trp Thr Gly Ser Gin Leu Ser Ile 325 330 335 Asn Asp Ser Leu Ile Gly Ile Ala Ala Gly Leu Leu Met Phe Ile Val 340 345 350 Pro Ala Asn Phe Lys Thr Giy Giu Arg Ilie Leu Asp Trp Arg Thr Ala 355 360 365 Gly Giu Leu Pro Trp Asp Val Leu Leu Leu Phe Gly Gly Gly Leu Ser 370 375 380 Leu Ser Ala Met Phe Thr Ser Thr Gly Leu Ser Leu Trp Ile Gly Glu 385 390 395 400 Leu Ala Lys Gly Leu Asp Ala Leu Pro Ile Phe Ile Leu Ile Phe Ala 405 410 415 Ilie Ala Val Leu Val Leu Phe Leu Thr Giu Phe Thr Ser Asn Thr Ala 420 425 430 Thr Ala Ala Thr Phe Leu Pro Ilie Met Giy Gly Val Ala Val Gly Ile 435 440 445 Gly Leu Thr Ala Gly Gly Giu Gin Asn Val Leu Leu Leu Thr Ile Pro 450 455 460 Val Ala Leu Ser Ala Thr Cys Ala Phe Met Leu Pro Val Ala Thr Pro 465 470 475 480 Pro Asn Ala Ilie Ala Phe Gly Ser Gly Tyr Ilie Lys Ilie Gly Glu Met 485 490 495 Val Lys Gly Gly Leu Trp Leu Asn Ile Ile Ala Val Ilie Leu Ile Thr 500 505 510 Ilie Phe Thr Tyr Phe Val Ala Ilie Pro Leu Phe Gly Ilie Met Leu 515 520 525 <210> 517 '.211> 1028 <z212> DNA <213> Corynebacteriun qiutamicum <220> <221> CDS <222> (1)..(1005) <223> FRXA00449 WO 01/00805 WO 0100805PCT/IBOO/00926 <400> 517 ctg gca cl Leu Ala LD 1 ttc gcc a( Phe Ala T) tca ctc g( Ser Leu G' tat atg t( Tyr Met St att ctt gq Ile Leu G.

gtg ttg a( Val Leu T1 cgt gaa cl Arg Giu LR cct cag g1 Pro Gin Vi 1: gtc ttc al Val Phe I.

130 aat gac t( Asn Asp SE 145 ccc gct ai Pro Ala A! ggc gaa cl Gly Giu Lo ctt tct g( Leu Ser A.

11 cta gct ai Leu Ala L, 210 att gct g1 Ile Ala V 225 gag Giu atg Met att Ile cat His att Ile 70 ttc Phe b9 t Arg ggt Gly act Th r 99 C Gly 150 acc Th r ga t Asp acc Thr gat Asp ttg Le u 230 act Th r ctg Leu ggc Gly gat Asp 9 ct Ala a ag Lys gaa Giu gtt Vali cta Leu 135 atc Ile 9c Gly gtt Val agc Ser gcc Ala 215 ttc Phe aac Asn 9ct Ala ttg Leu 99 t Gly atc Ile atc Ile ccg Pro gca Al a 125 ca 9 Gin atg Met tg9 Trp 99 c Gly t 99 Trp 205 ctc Le u tcc Ser ca a Gin tcc Ser ctt Leu ca 9 Gin gcg Al a cct Pro tgg Trp 110 ct 9 Le u ctc Leu ttt Ph e c9 t Arg 999 Gi y 190 atc Ile atc Ile aac As n aag Lys a tc Ile gct Ala tgg T rp t 99 Trp 99 c Gly act Th r gct Ala t ct Ser atc Ile act Thr 175 ctt Leu 99t Gly ttc Phe acc Thr aaa Lys 99 t Gly 9 cg Ala a tg Met ctt Leu gga Gly gca Ala t99 Trp atc Ile gtt Val1 160 g ca Ala t ca Ser gaa Gi u gcc Ala gca Al a 240 WO 01/00805 aca gcg qca acc ttc ctg cca atc atg Thr Ala Ala Thr Phe Leu Pro Ilie Met 245 gga ctg acc gca ggt gqc gag cag aat Gly Leu Thr Ala Gly Gly Glu Gin Asn 260 265 gtc gca ctg tcc gca acc tgt gcg ttc Val Ala Leu Ser Ala Thr Cys Ala Phe 275 280 cca aac gcg att gca ttc ggc tcc ggc Pro Asn Ala Ile Ala Phe Gly Ser Gly 290 295 gtc aag ggt gqt ctg tgg ctq aac atc Val Lys Gly Gly Leu Trp Leu Asn Ile 305 310 att ttc acc tac ttc gta gcq atc cca Ile Phe Thr Tyr Phe Val Ala Ile Pro 325 taaaagttaa caggcccgca gtc 518 <21i> 335 <2i2> PRT <2i3> Corynebacterium glutamicum <400> 518 Leu Ala Leu Thr Ala Giu Thr Val Gly gta ValI acc Thr 270 g ca Ala gg C Gly ctc Leu atg Met PCTIBOOIOO926 atc 768 Ile cca 816 Pro cct 864 Pro atg 912 Met acg 960 Thr 320 1005 1028 Al a Leu Met Leu Leu Glu Gin Phe 130 Asp Thr Gly Ser Gly Thr Le u Val 115 Ile Ser Gi y Thr Giu Val1 Thr Ile 100 Thr Pro Leu Le u Leu Ser Pro Val1 Lys Val Le u Ile Met Ile His Ile Phe Arg Gly Thr Gly Leu Gly Asp 55 Ala Lys Glu Val1 Leu 135 Ile Ser Thr Ile Val Pro Ile I le 120 Asp Ala Gly 10 Ala Pro Ile Phe Met 90 Glu Al a Th r Gly Met Tyr Asn Ci y Th r 75 Lys Met Ala Giy Le u L ys Ser Ala Phe Ile Giu Gly Ala Ser 140 Le u Gin Ser Leu Gin Al a Pro Trp 110 Leu Leu Phe Lys Ile Al a Trp Trp Gly Thr Al a Ser Ile Lys Gly Al a Met Leu Gly Ala T rp Ile Val1 WO 01/00805 PCT/IBOO/00926 145 150 155 160 Pro Ala Asn Phe Lys Thr Gly Giu Arg Ile Leu Asp Trp Arg Thr Ala 165 170 175 Gly Giu Leu Pro Trp Asp Val Leu Leu Leu Phe Gly Gly Gly Leu Ser 180 185 190 Leu Ser Ala Met Phe Thr Ser Thr Gly Leu Ser Leu Trp Ile Gly Giu 195 200 205 Leu Ala Lys Gly Leu Asp Ala-Leu Pro Ile Phe Ile Leu Ile Phe Ala 210 215 220 Ilie Ala Val Leu Val Leu Phe Leu Thr Glu Phe Thr Ser Asn Thr Ala 225 230 235 240 Thr Ala Ala Thr Phe Leu Pro Ilie Met Gly Gly Val Ala Val Gly Ilie 245 250 255 Gly Leu Thr Ala Gly Gly Glu Gin Asn Val Leu Leu Leu Thr Ilie Pro 260 265 270 Val Ala Leu Ser Ala Thr Cys Ala Phe Met Leu Pro Val Ala Thr Pro 275 280 285 Pro Asn Ala Ile Ala Phe Gly Ser Gly Tyr Ile Lys Ile Giy Glu Met 290 295 300 Val Lys Gly Gly Leu Trp Leu Asn Ile Ile Ala Vai Ile Leu Ile Thr 305 310 315 320 Ilie Phe Thr Tyr Phe Val Ala Ilie Pro Leu Phe Gly Ilie Met Leu 325 330 335 <210> 519 <211> 448 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(448) <223> FRXA01755 <400> 519 tcgttggctt acatggtcat tgatgaccgg gctgaatatg tgagaaaatc catcccttct ttaagcaagg gagtgaatta cagaaaagga ttgttcagca atg agc aca cct gac 115 Met Ser Thr Pro Asp 1 att aaa gaa ggc tcg gca gaa tca ccg gqc gaa gta atg gtc gtt gga 163 Ilie Lys Glu Giy Ser Ala Glu Ser Pro Gly Giu Val Met Val Vai Giy 15 gac agg cga gag tgg cgt cga caa gca acc ggc atc att gcc ggc ctc 211 Asp Arg Arg Glu Trp Arg Arg Gin Ala Thr Gly Ile Ile Ala Gly Leu 30 WO 01/00805 PCTLBOO/00926 qtc tta gcc gcc ctg gtc tat ctt ctc ttc ccc tcg aac tcc gtg gaa 259 Val Leu Ala Ala Leu Val Tyr Leu Leu Phe Pro Ser Asn Ser Val Giu 45 acc gtc atg caa tcc agt ggc gtc gat cca gaa act gaa tac acc aac 307 Thr Val Met Gin Ser Ser Gly Val Asp Pro Giu Thr Glu Tyr Thr Asn 60 aac gcg atq cgt ctt act gct gca gtc aca atc ttg atg gca gtg tgg 355 Asn Ala Met Arg Leu Thr Ala Ala Val Thr Ilie Leu Met Ala Val Trp 75 80 tgg atq aca gaa gca atc cca. cta gca gca acc gca. ctt atc ccg ttg 403 Trp Met Thr Giu Ala Ilie Pro Leu Ala Ala Thr Ala Leu Ile Pro Leu 95 100 gtt gca ttc cct qct ttc cag gtt gtg gac ttt ggg aag gca gca 448 Val Ala Phe Pro Ala Phe Gin Val Val Asp Phe Gly Lys Ala Ala 105 110 115 <210> 520 <211> 116 <212> PRT <213> Corynebacteriun giutamicum <400> 520 Met Ser Thr Pro Asp Ile Lys Glu Gly Ser Ala Glu Ser Pro Gly Giu 1 5 10 Val Met Val Val Gly Asp Arg Arg Giu Trp Arq Arg Gin Ala Thr Gly 25 Ile Ile Ala Gly Leu Val Leu Ala Ala Leu Val Tyr Leu Leu Phe Pro 40 Ser Asn Ser Val Giu Thr Val Met Gin Ser Ser Gly Val Asp Pro Glu 55 Thr Glu Tyr Thr Asn Asn Ala Met Arg Leu Thr Ala Ala Val Thr Ile 70 75 Leu Met Ala Vai Trp Trp Met Thr Giu Ala Ilie Pro Leu Ala Ala Thr 90 Ala Leu Ilie Pro Leu Val Ala Phe Pro Ala Phe Gin Val Val Asp Phe 100 105 110 Gly Lys Ala Ala 115 <210> 521 <211> 912 <212> DNA <213> Corynebacterium giutamicum <220> <221> COS <222> (101)..(889) <223> RXA00269 WO 01/00805 WO 0100805PCTA~BOO/00926 (400> 521 tgcgatcttg gtggttgtcg ccatgctgct gccgaggtgg cgtgcgaagt tctccaaggc accgaagcct aagcaaccag tagcagtgga ggcttaagac atg tta tcc atc aac Met Leu Ser Ile Asn gga Gly ttg Leu atc Ilie Giy gt a Val1 ttc Phe aat Asn qca Ala ctt Leu tct Ser 150 caa Gin cag Gin gga Gly att Ilie ca g Gin gg t Giy cgt Arg aac Asn cag Gin ctg Leu ct g Leu gag Giu 135 ggc Gly cct Pro cgt Arg aat Asn tct S er ca g Gin tct Se r ttg Leu aag Lys gat Asp gcc Aia acc Th r 120 ct g Leu ggt Giy aaa Lys gca Aia ctg Leu aag Lys ctc Leu aac Asn ctt Leu atg Met cc t Pro atc Ile 105 t cg Ser gg t Giy ca g Gin atc Ilie gag Gi u 185 act Thr a cg Thr aaa Lys ggt Gi y gt t Vali tca Ser ctg Leu gcg Aila aa g Lys ctg Leu cgt Arg atg Met 170 ct t Leo a cg Thr ttc Phe ctc Leu gcg Aia gat Asp gag Giu 75 g cc Aila t tg Leu cgc Arg gaa Glu ca g Gin 155 ctg Leu gtg Val1 ctt Leu ttc Phe ga t Asp 9gt Gly t cc Ser 60 cac His ggc Giy ct g Leu cgt Arg aac As n 140 gca Ala ttg Leu acc Thr atg Met ccc ggc act Pro Giy Thr 15 atg gct gag Met Aia Glu 30 aaa tcc acg Lys Ser Thr 45 ggc gag att Gly Giu Ile aag cgt gcc Lys Arg Ala acc gcg ccg Thr Aia Pro 95 cgc ggc aag Arg Gly Lys 110 gag caa ttc Gio Gin Phe 125 agg ctc act Arg Leo Thr ttg tcc ctg Leu Ser Leo gat gag cac Asp Glu His 175 acc ttg acc Thr Leo Thr 190 gtc acg cac Vai Thr His 205 atg atg cat gtg Vali Gly ct g Leo t cc Ser cgc Arg 80 aat As n cg c Arg aag Lys gcc Ala ct g Leo 160 acc Thr ga a Glu aac Asn ga a 1 aat Asn gat Asp ctc Leo atc Ile tac Tyr ctc Leo cg t Arg ca g Gin aaa Lys 145 atg Met gca Ala aag Lys atg Met gag Gio ttt Phe aac As n ga c Asp atc Ile acc Thr gga Giy gaa Glu 130 gt t Vali gct Ala 9cg Aia atc Ile gaa Glu cgc cgc Arg Arg gtc acc Val Thr gct gtt Ala Val ggc aac Giy Asn ggc cgc Gly Arg att gaa Ile Gio 100 ttg ggc Leo Giy 115 ctt gag Leo Giu ggt ttg Gly Leu ggt ttt Gly Phe ctt gat Leo Asp 180 gtg gca Val Ala 195 cag gca Gin Ala gcg Ala gt c Vali t ct Ser aa g Lys gtt Val1 gag Glu ttt Ph e cg c Arg ct c Leo act Th r 165 cca Pro ga t Asp att Ile 115 163 211 259 307 355 403 451 499 547 595 643 691 739 787 200 210 cgc ctg ggc aat cgc ctg atc ggc cag att gtc tac WO 01/00805 PCTIBOO/00926 Arg Leu Gly Asn Arg Leu Ile Met Met His Giu Gly Gin Ile Val Tyr 215 220 225 cag gca gat cag gct acc aag tcg aag ttg act gtg cgc gat ttg ctg 835 Gin Ala Asp Gin Ala Thr Lys Ser Lys Leu Thr Val Arg Asp Leu Leu 230 235 240 245 cag gag ttc gcc aac atc aag ggc gca aca ttg tct gac aag gcg ttc 883 Gin Giu Phe Ala Asn Ilie Lys Gly Ala Thr Leu Ser Asp Lys Ala Phe 250 255 260 ctc ggc taaaaagagc ttgctttacg acg 912 Leu Gly (210> 522 <211> 263 <212> PRT <213> Corynebacterium giutamicum <400> 522 Met Leu Ser Ilie Asn Giy Ilie Ser Lys Thr Phe Phe Pro Gly Th r Val 1 5 10 Asn Giu Arg Arg Ala Leu Gin Gin Leu Lys Leu Asp Met Ala Giu Giy 25 Asp Phe Val Thr Vai Ilie Gly Ser Asn Gly Ala Gly Lys Ser Thr Leu 40 Leu Asn Ala Val Ser Gly Arg Leu Leu Val Asp Ser Giy Giu Ilie Ser 55 Ile Asp Gly Asn Lys Vai Asn Lys Met Ser Giu His Lys Arg Ala Arg 70 75 Tyr Ile Gly Arg Val Phe Gin Asp Pro Leu Ala Giy Thr Ala Pro Asn 90 Leu Thr Ile Giu Giu Asn Leu Ala Ilie Ala Leu Leu Arg Gly Lys Arg 100 105 110 Arg Gly Leu Gly Phe Ala Leu Thr Ser Lys Arg Arg Giu Gin Phe Lys 115 120 125 Gin Giu Leu Giu Arg Leu Giu Leu Gly Leu Giu Asn Arg Leu Thr Ala 130 135 140 Lys Val Giy Leu Leu Ser Giy Giy Gin Arg Gin Ala Leu Ser Leu Leu 145 150 155 160 Met Aia Gly Phe Thr Gin Pro Lys Ile Met Leu Leu Asp Giu His Thr 165 170 175 Ala Ala Leu Asp Pro Gin Arg Ala Giu Leu Val Thr Thr Leu Thr Giu 180 185 190 Lys Ilie Val Ala Asp Gly Asn Leu Thr Thr Leu Met Val Thr His Asn 195 200 205 WO 01/00805 PCT/IBOO/00926 Met Glu Gin Ala Ile Arg Leu Gly Asn Arg Leu Ile Met Met His Giu 210 215 220 Gly Gin Ile Val Tyr Gin Ala Asp Gin Ala Thr Lys Ser Lys Leu Thr 225 230 235 240 Vai Arg Asp Leu Leu Gin Glu Phe Ala Asn Ile Lys Gly Ala Thr Leu 245 250 255 Ser Asp Lys Ala Phe Leu Gly 260 <210> 523 <211> 817 <212> DNA <213> Corynebacterium qiutarnicum <220> <221> COS <222> (101)..(817) <223> RXA00369 <400> 523 ggcgggaaat tacaatcagg catcggcgtt gtcgttgatg tttcjcgatta tcggtatcgt ggcqctcgcg ttgacgqgc qcagccagaa ggagttttag gtg tca tcg atc aaa Val Ser Ser Ile Lys ttg Leu ga t Asp C Cg Pro gtg Val1 cat His 9 C9 Ala g cg Ala ga t Asp aat Asn gqg Gly cca Pro ccg Pro t gg T rp agt Ser tta Leu ttq' Leu tcc Ser agt Ser ccq Pro ccg Pro qgt Gly 105 cg C Arg gaa Glu 30 atq Met ctc Le u atg Met gcc Ala t cg Ser 110 gac Asp gaa G iu ttq Leu atc Ile Gly aag Lys ata Ile gga Gly ttt Phe gg c Gly gcc Al a atg Met 80 aat Asn aqc Ser 99 C Gly 1 acc Th r gtg ValI a cc Thr ggc Gi y gtg ValI gtg Val1 aaa Lys agt S er ttc Phe gt g ValI atc Ile ga a Gi u ttt Phe gga Gly cgc Ar g cgc Arq 130 9gg Gly ctc Leu 9 cg Ala ga a Giu cag Gin tac Tyr gtq Val1 115 aga Arg ctg Leu a tc Ilie qqq Gi y atg Met cag Gin ccg Pro 100 gag Glu ccg Pro caa Gin 999 Gly ttt Phe a cg Thr cat His ctg Leu cgc Arg 9C Ala 115 163 211 259 307 355 403 451 499 acg ctc gcg ctg gtg ggg ctt gag ggg ttc Thr Leu Ala Leu Val Gly Leu Glu Gly Phe WO 01/00805 agc ctg tct ggt ggt caa cgt cag c Ser Leu Ser Gly Gly Gin Arg Gin A 135 140 atc gcc gac ccc acc gtg ctg ctt c Ilie Ala Asp Pro Thr Val Leu Leu L.

150 155 gac gaa ccg ctg cga gac gct tta c Asp Glu Pro Leu Arq Asp Ala Leu A 170 cgg cgc gaa ggc ctc act act gtg c Arg Arg Glu Gly Leu Thr Thr Val H 185 1 gcg atc tcc atc qct qac cgc atc g Ala Ilie Ser Ilie Ala Asp Arg Ilie V 200 205 caa cag gta gcc acc cct act gag c Gin Gin Val Ala Thr Pro Thr Giu 1 215 220 gat gtt gcc cga ttc atc gtc gac Asp Val Ala Arg Phe Ilie Val Asp 7 230 235 <210> 524 <211> 239 (212> PRT <213> Corynebacterium giutamicum <400> 524 Val Ser Ser Ile Lys Leu Arg Asp 1 5 Thr Phe Gly Leu Gin Asp Ile Asn Val Val Leu Ilie Giy Pro Ser Gly 40 Thr Ile Ala Gly Phe Val Giu Pro 55 Gly Glu Giu Met Thr His Val Pro 70 Val Phe Gin Gin His Ala Val Trp Val Gly Tyr Pro Leu Ala Arg Ser 100 Lys Arg Vai Giu Arg Thr Leu Ala 115 120 Ser Arg Arq Pro Ala Ser Leu Ser PCTJIBOO/00926 .1 547 :gg Lrg :tc ,eu :ga r g a c is .90 ;tc al1 :tc ~eu ;cc l a Leu Leu 25 Ser Ser Pro Pro Gliy 105 Leu Gly gtg Val ga C Asp cg C Arg 175 gtg Vai gta ValI ct t L eu acc Thr Ser 10 Lys Giy Ser Glu His 90 Gin Val Gly gcg Al a gag Gi u 160 gaa Giu a cg Thr ctc Leu tcc Ser ctt Leu 145 gcc Ala ctc Leu cat His ggc GI y gcc Ala 225 gcg Ala ct c Leu g ta ValI ga c Asp aac Asn 210 ccc Pro cg c Arg tcc Ser tct Se r cg c Arg 195 ggt Gi y gct Ala gcc Ala gcc Al a ttg Leu 180 qcc Ala cga Arg act Th r Val Ile Ly s Gi y Arq 75 Met L ys Gly Gin Ser Glu Thr Ser Arg Ser Gly Leu Arg Phe Pro Th r Val1 Arq Val1 Ala Giu 125 Gin Arg Giu Met Leu Met Al a Ser 110 Gly Arg Asp Giu Leu Ile Gly Lys Ile Ph e Val Gly Phe Gly Ala Met Asn Se r Gly Al a WO 01/00805 130 135 Leu Ala Arg Ala Ile Ilie Ala Asp Pro Thr Vai 145 150 155 Ala Leu Ser Ala Leu Asp Glu Pro Leu Arg Asp 165 170 Leu Val Ser Leu Thr Arg Arg Glu Gly Leu Thr 180 185 His Asp Arg Ala Giu Ala Ile Ser Ile Ala Asp 195 200 Gly Asn Giy Arg Ile Gin Gin Val Ala Thr Pro 210 215 Ala Pro Ala Thr Aia Asp Vai Ala Arg Phe Ile 225 230 235 <210> 525 <211> 1653 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1630) <223> RXA02073 <400> 525 tcattgtttt agaggatggc caattgacca tgatggatac acaatgcqtt tttccgcacc qctgtgatqq aggaagaaca PCTIBOO/00926 Leu Arg His 190 Val1 Leu Ala Asp Glu 160 Arg Glu 175 Val Thr Vai Leu Leu Ser Th r ctc Leu ctg Leu 9CC Ala.

atc Ile gag Glu cgc Arg aaa L ys Ca 9 Gin cgc Ar g atc Ile cac His 75 ga C Asp Ctt Leu g ca Al a tcc Ser g ct Ala 999 Gly 80 aaa Lys acccagcaac gtttcccagc atg att tcc cga ctt Met Ile Ser Arg Leu 1 gga gcc tcc acg ctc Giy Aia Ser Thr Leu ctc att gtq ttc ccc Leu Ile Vai Phe Pro ctg ctc gcc gtc gcc Leu Leu Ala Val Ala gtc tqt cgc tgq ggt Val Cys Arg Trp Gly ctg ctt gcc cac atg Leu Leu Ala His Met ggt tcc ccc tcg ccg Gly Ser Pro Ser Pro 100 WO 01/00805 WO 0100805PCTLBOO/00926 atc Ile tcc Ser gtg Val1 gtt Val1 150 ccc Pro Ca c His aac Asn gag Gi u atc Ile 230 ttg Leu ctg Le u atg Met gcc Al a ggc Gly 310 acc Th r ca C His atc Ile ctg Le u 135 gct Al a ttg Le u atc Ile t ca Ser caa Gin 215 cgt Arg gtt Val ggc Gly C ct Pro cct Pro 295 ccg Pro g tg Val ggc Gly gaa Glu 120 ctc Le u gg t Gly att Ile gcc Al a cat His 200 ca a Gin cag Gin gct Al a atc Ile acc Thr 280 qt t Val1 ctt Le u tct Ser agc Se r 105 9 ta Val agt Ser cta Leu gga Giy ca g Gin 185 gga Gly ctg Le u ggc Giy ct g Leu tcc Ser 265 g cg Al a gct Al a aag Lys tta Leu ggt Gly ttc Ph e g cg Ala att Ile aaa Lys 170 cac His g cg Ala gaa Glu g ca Ala gtt ValI 250 cct Pro tta Leu atc Ile ctg Leu gag Glu 330 tcg Ser ttc Ph e gga Gi y g9t Gi y 155 caa Gin ct C Leu caa Gin caa Gin gca Al a 235 cct Pro gcg Ala aac Asn gct Ala cg a Arg 315 gtt Val1 atc Ile gc9 Ala 99 c Gly 140 tta Le u tcc Ser acc Thr gcc Al a gt t Val 220 ct t Le u cat His ttg Leu agt Ser gaa Giu 300 att Ile 9 ca Al a a tg Met cac His 125 gtg Val ctc Leu t ca Ser gaa Giu aca Thr 205 gtg Val1 tta Leu gtg ValI gat Asp g cg Al a 285 cct Pro t ct Ser gct Ala tct S er 110 acc Th r atc Ile ggt Gi y agc Ser ga t Asp 190 cgg Arg acc Th r t 99 Trp ggc Gly gca Ala 270 ca g Gin gac Asp aga Arg ggt Gly gtt ValI att Ile a cg Thr gtc Val1 ag t Ser 175 gcc Ala tta Leu cg a Arg C cg Pro att Ile 255 gtt Val1 Arg gag Giu gtt ValI gaa Giu 335 gcc Ala ggg Gi y ctg Leu ctC Leu 160 gaa Giu gcc Al a aat Asn ca g Gin tgg Trp 240 gtt Val gag Glu tat Tyr C cg Pro cc a Pro 320 cac His a cc Thr c~t pro gca Al a 145 atc Ile gc c Ala ggc Gi y gcg Al a ggc Gly 225 ata Ile g ca Al a gga Gly ttc Phe aag Lys 305 gtc ValI atc Ile cg t Arg aca Thr 130 a cg Thr g cg Ala aca Thr agg Arg ctc Leu 210 ttg Leu tca Ser gcc Al a ttt Phe cag Gin 290 cct Pro agc Ser ggc Gly gac Asp 115 gtc ValI ct c Leu tgg Trp tca Ser ctt Le u 195 gag Glu atc Ile gct Al a gcg Ala get Al a 2.75 atc Ile ttg Le u gca Ala atc Ile att Ile acc Thr ga t Asp ttg Leu cgt Arg 180 gaa Glu gtg Vai gtc Val1 gtg Val1 att Ile 260 cg c Arg atc Ile ccc Pro aag Lys atc Ile 340 aac Asn gca Al a ccc Pro atc Ile 165 gga Gi y atc Ile aaa Lys ggt Gly t tg Leu 245 atc Ile acc Thr ga t Asp aaa Lys ggc Gly 325 gga Gly 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 t cc S er gcg Ala gag Giu aaa Lys 390 aat As n t cg Ser gga Gly cag Gin caa Gin 470 atc Ile WO 01/00805 agc ggt agt Ser Gly Ser 345 caa cta cgg Gin Leu Arg 360 gtt tca tcg Val Ser Ser 375 tct gtg ctg Ser Vai Leu cca gag ctg Pro Giu Leu ata agc gaa Ile Ser Glu 425 cag caa caa Gin Gin Gin 440 gta ctg att Vai Leu Ilie 455 gcg gat ctt Aia Asp Leu atc att gcg Ilie Ilie Ala gg a Giy tct Ser g cg Al a ttt Phe tct Ser 410 ctg Leu C9g Arg gt c Val tcc Ser cac His 490 aaa Lys gga Gly gag Glu aga Ar g 395 gaa Giu cc t Pro ctc Leu ga t Asp cag Gin 475 cgc Arg tcc Ser acc Thr ctt Leu 380 gca Al a ga t Asp tta Le u tgC Cys gaa Glu 460 act Thr aca Th r act Thr atc Ile 365 cgc Arg agc Se r gaa Giu ga t Asp ctg Leu 445 gcc Aia ctg Leu gct Aila ttg gcc Leu Ala 350 acc atc Thr Ile aaa tcc Lys Ser gtg ctg Vai Leu qca agg Ala Arg 415 gct gac Ala Asp 430 gcg cgt Aia Arg 2CC agc Thr Ser gcc acg Ala Thr gca tta Aia Leu 495 atc Ile gt t Val 370 ct g Leu tta Leu ttg Le u cgc Arg gca Aia 450 ga t Asp gac Asp gtg ctc Leu 355 ga t Asp gt t Vali Cgg Arg agg Arg cta Le u 435 cgc Arg gcg Aia acc Thr gat aag Lys atc: Ile gag Giu a tg Met ttg Leu 420 tct Ser acc Th r ctc Leu acg Thr C9g PCT/IBOO/00926 ctg 1171 Leu gca 1219 Ala cag 1267 Gin ggc 1315 Gly 405 gcg 1363 Al a ggC 1411 Gly cct 1459 Pro aat 1507 As n gtg 1555 Val1 485 ata 1603 Thr His Val Asp Arg Ile 500 att gac ctg gaa gaa atc aaa aat ccc tgatccttaa cgcggatcag Ile Asp Leu Giu Glu Ile Lys Asn Pro 505 510 ggg <210> 526 <211> 510 <212> PRT <213> Corynebacteriun giutamicum <400> 526 Met Ilie Ser Arg Leu Leu Gin Leu Ala Lys Lys Vai Trp Pro Glu Leu 1 5 10 GSly Ala Ser Thr Leu Leu Arg Leu Leu Asn Gin Leu Leu Thr Ala Ala 25 Leu Ile Val Phe Pro Ala Trp Vai Leu Ser Arg Lys Pro Asp Ile Ser 40 1650 1653 WO 01/00805 WO 0100805PCTJIBOO/00926 Leu ValI Leu GI y Th r Pro Al a 145 Ile Ala Gly Ala Gly 225 Ile Al a Gly Phe Lys 305 Val1 Ile Leu Le u Cys Le u Ser Arg Th r 130 Th r Ala Th r Arg Leu 210 Leu Ser Ala P he Gin 290 Pro Ser Gly Ile Ala Arg Ala Pro Asp 115 Vai Leu T rp Ser Leu 195 Glu Ile Al a Al a Ala 275 Ile Le u Ala Ile Le u 355 Val1 Trp His S er 100 Ile Thr Asp Leu Arg 180 Glu Val1 Val1 Val1 Ile 260 Arg Ile Pro Lys I le 340 Ly s Al a Gi y Met Pro As n Ala Pro Ile 165 Gly Ile Lys Gly Leu 245 Ile Th r Asp Lys Gly 325 Gly Leu Ile Glu 70 Arg Ile Ser ValI Val1 150 Pro His As n Giu Ile 230 Leu Leu Met Ala Gly 310 Thr Ser Ala Ile 55 Gin Val1 His Ile Leu 135 Al a Leu Ile Ser Gin 215 Arg Val Gly Pro Pro 295 Pro Val1 Ser Gin Met Val1 Met Gly Glu 120 Leu Gly Ile Ala His 200 Gin Gin Al a Ile Th r 280 ValI Le u Se r Gly Leu 360 Al a C ys Leu Ser 105 Val1 Ser Leu Gly Gin 185 Gly Leu Gly Leu Ser 265 Ala Ala Lys Leu Ser 345 Arg Leu Gly Tyr 90 Gly Ph e Ala Ile Lys 170 His Al a Giu Al a Val1 250 Pro Leu Ile Leu Glu 330 Gly S er Ile His 75 Asp Ser Phe Gly Gly 155 Gin Le u Gin Gin Ala 235 Pro Al a As n Ala Arg 315 Val Lys Gly Al a Arg Ala Ile Ala Gly 140 Leu Ser Th r Ala Val1 220 Le u His Leu Ser Glu 300 Ile Ala Ser Thr Le u Al a Leu Met His 125 Val1 Le u S er Giu Thr 205 Val Leu Val1 Asp Ala 285 Pro Ser Ala Thr Ile 365 Thr Al a Val1 Ser 110 Thr Ile Gly S er Asp 190 Arg Th r Trp Gly Ala 270 Gin Asp Arg Gly Leu 350 Th r Ala Phe His Val1 Ile Thr Val1 Ser 175 Ala Le u Arg Pro Ile 255 ValI Arg Glu ValI Glu 335 Al a Ile Al a Gly Lys Al a Gi y Leu Le u 160 Glu Ala Asn Gin Trp 240 Val1 Giu Tyr Pro Pro 320 His Lys Gly WO 01/00805 PCTJIBOO/00926 Gly Val ASP Ile Ala Giu Val Ser Ser Ala Glu Leu Arg Lys Ser Val 370 375 380 Thr Leu Val Glu Gin Lys Ser Val Leu Phe Arg Ala Ser Val Leu Giu 385 390 395 400 Asn Leu Arg Met Gly Asn Pro Giu Leu Ser Giu Asp Glu Ala Arg Glu 405 410 415 Ala Leu Arg Leu Ala Ser Ilie Ser Giu Leu Pro Leu Asp Ala Asp Ala 420 425 430 Leu Arg Leu Ser Gly Gly Gin Gin Gin Arg Leu Cys Leu Ala Arg Ala 435 440 445 Leu Ala Arg Thr Pro Gin Val Leu Ilie Val Asp Glu Ala Thr Ser His 450 455 460 Gin Asp Ala Leu Asn Gin Ala Asp Leu Ser Gin Thr Leu Ala Thr Leu 465 470 475 480 Lys Asp Thr Thr Val Ilie Ilie Ile Ala His Arg Thr Ala Ala Leu Thr 485 490 495 His Val Asp Arg Ile Ile Asp Leu Giu Glu Ile Lys Asn Pro 500 505 510 <210> 527 <211> 1142 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(1119) <223> RXA01399 <400> 527 att ctc agc ccc gca acc gca ctt gtc ctg gcg att qga ttg att qct 48 Ilie Leu Ser Pro Ala Thr Ala Leu Val Leu Ala Ile Gly Leu Ile Ala 1 5 10 gct gca att atc cct ccc ctg ctt gct gct cgc gga gtt aaa aca gcc 96 Ala Ala Ilie Ile Pro Pro Leu Leu Ala Ala Arg Gly Val Lys Thr Ala 25 gaa gcc cqg cgc gct gaa tcc agc gaa gcc tac ttg agt tcc ttg gat 144 Glu Ala Arg Arg Ala Glu Ser Ser Glu Ala Tyr Leu Ser Ser Leu Asp 40 cag gtg ctg tcc aac cag gcg gcg ctt cgt qtt cgt ggt gaa atg ccg 192 Gin Val Leu Ser Asn Gin Ala Ala Leu Arg Val Arg Gly Glu Met Pro 55 gcc gct ctg tcc aag gcg gat gtg gct gcg cgt tcc tat tct tct tca 240 Ala Ala Leu Ser Lys Ala Asp Val Ala Ala Arg Ser Tyr Ser Ser Ser 70 75 ctg gag gca ggc gcg aaa gac act gcc att ggc gca gcg agt tcc ctg 288 Leu Glu Ala Gly Ala Lys Asp Thr Ala Ilie Gly Ala Ala Ser Ser Leu WO 01/00805 WO 0100805PCTAIBOO/00926 t gg Trp ctg Leu ctg Leu gcg Ala 145 atc Ile ga c Asp gac Asp atc Ile gcg Ala 225 aat As n cac His gaa Glu tca Ser tca Ser 3 05 g cc Al a att Ile tat Tyr ctt Leu 130 att Ile tcg Ser cag Gin cta Leu atc Ile 210 ggg Gly cc t Pro att Ile gca Al a gag Glu 290 gg t Gly cg C Arg cac His gca Ala 115 tca Ser gct Al a gcg Al a ccc Pro g gc Gly 195 gca Ala ct g Leu t cc Ser ttt Ph e acc Thr 275 tgg Trp gcC Ala gta Val ggt Gi y 100 gat Asp ctc Leu cg t Arg ctg Leu gta Val1 180 a cg Thr ccc Pro ttg Le u gag Glu gcc Ala 260 gac Asp gtt Val gat Asp cta Le u ttc Phe gga Gly gca Ala acc Thr cca Pro 165 tta Leu aqc Ser tct Ser gaa Giu ttg Leu 245 acc Thr gcg Al a ca a Gin ag t Se r cta Leu 325 act Thr agc Ser gct Ala cgc Arg 150 ga a Giu cg C Arg aac Asn gga Gly Cc t Pro 230 aaa L ys act Thr gaa Giu ggt Gly ctc Leu 310 ag t Ser gtc ValI cat His ttC Ph e 135 gcc Al a tct Ser gcc Al a ctg Leu act Th r 215 cg t Arg aac As n gtc ValI a tg Met tta Leu 295 tcg *Ser gat *Asp att tca Se r 120 gag Glu g ca Ala gtc Val gag Glu gat Asp 200 ggc Gly gga Gi y gcc Ala cga Arg aca Th r 280 ccc Pro gga *Gly *gca 4Ala ~g t Gly 105 ccg Pro gct Al a gat Asp tct Ser aat As n 185 ctc Leu aaa Ly s gg C Gly gtg Val ga t Asp 265 t cg S er gat Asp gg t Gl y cca Pro 90 Val1 cag Gin gt C Val gcc Al a ctt Leu 170 ct a Le u acc Thr acg Thr caa Gin ctg Leu 250 aac As n atc Ile gg t Gi y ca g Gin att Sle 330 ctc Le u tgg T rp tct Ser acc Thr 155 gag Giu gtt Val1 ttt Ph e acc Thr gtg Val 235 ttc Ph e tta Leu ctg Leu ctt Leu cg c Arg 315 ctg Leu atg Met ttt Phe gtt Val 140 agg Arg ct t Leu tat Tyr gg t Gly ctg Leu 220 ct t Leu ag t Ser g ca Al a gaa Giu Gly 300 cgc Arg ctt Leu gtt Val ggt Gly 125 ctc Leu agg Arg cgc Arg gga Gly tca Ser 205 ctg Leu atc Ile cca Pro ctc Leu cat His 285 act Thr cqc Ar g ttg Leu tcc Ser 110 gtg Val1 ccc Pro ctt Leu a cg Thr tgg Trp 190 cga Arg ctc Leu gac Asp gaa Glu gga Giy 270 gtt Val1 gtc Val1 ct g Leu gat Asp~ gcg Ala ttg Leu gat Asp gcg Ala gcc Ala 175 gac Asp cat His aca Thr gg c Gly gat Asp 255 g ca Ala gg t Gly ctt Leu ctc ILeu gaa Glu 335 t ca Ser g tg Val1 gc t Al a gaa Glu 160 tct Ser agc Ser ga a Gi u ctt Leu acc Th r 240 g cc Al a ccg Pro t tg Leu gat Asp ctt Leu 320 ccc Pro 336 384 432 480 528 576 624 672 720 768 816 864 912 960 1008 WO 01/00805 acc gag cac ctc gac Thr Glu His Leu Asp 340 gcc tcc gat gaa ctc Ala Ser Asp Giu Leu 355 gtq agq cat gtg agg Val Arg His Val Arg 370 <210> 528 <211> 373 <212> PRT <213> Corynebacteriu

P

act gca ggc tcc tct gaa atc ttg tct atg ctg Thr Ala Gly Ser Ser Giu Ile Leu Ser Met Leu 345 350 cct ggt aaa aga gct agg aga acc gta gtg att Pro Gly Lys Arg Ala Arg Arg Thr Val Val Ile 360 365 taattttgcc ccgctgtctt ctg im glutamicum CT11B00100926 1056 1104 1142 <400> 528 Ile Leu Ser Pro Ala Thr Ala Leu Val Leu Ala Ile Gly Leu Ile Ala 1 Ala Glu Gin Ala Leu Trp Leu Leu Ala 145 Ilie Asp Asp Ile Al-a Ala Val1 Al a Giu Ile Tyr Leu 130 Ilie Ser Gin Leu Ilie 210 Ile Arg Leu Leu Ala His Al a 115 Ser Ala Al a Pro Gly 195 Ala Ile Arg Ser Se r Gly Gly 100 Asp Leu Arg Leu Val1 180 Th r Pro 5 Pro Pro Ala Glu Asn Gin Lys Ala 70 Ala Lys Phe Thr Gly Ser Ala Ala Thr Arg 150 Pro Glu 165 Leu Arg Ser Asn Ser Gly Le u Ser Ala 55 Asp Asp Val His Phe 135 Ala Ser Al a Leu Th r 215 Leu Ala 25 Ser Giu 40 Ala Leu Val Ala Thr Ala Ilie Gly 105 Ser Pro 120 Glu Ala Ala Asp Val Ser Glu Asn 185 Asp Leu 200 Gly Lys 10 Al a Ala Ar g Ala Ile 90 Val1 Gin Val1 Ala Leu 170 Leu Thr Th r Ar g Tyr Val Arg 75 Gly Leu T rp Ser Thr 155 Giu Val1 Ph e Th r Gly Leu Arg Ser Al a Met Ph e Val 140 Arg Leu T yr Gly Leu 220 Val1 Ser Gly Tyr Ala Val Gly 125 Leu Arg Arg Gly Ser 205 Le u Lys Thr Ser Leu Giu Met Ser Ser Ser Ser Ser Ala 110 Val Leu Pro Asp Leu Ala Thr Ala 175 Trp Asp 190 Arg His Leu .Thr Al a Asp Pro Ser Leu Ser Val1 Ala Glu 160 Ser Ser Giu Leu WO 01/00805 Ala Gly Leu Leu Giu Pro Arg Gly Gly Gin Val 225 230 235 Asn Pro Ser Glu Leu Lys Asn Ala Val Leu Phe 245 250 His Ile Phe Ala Thr Thr Val Arg Asp Asn Leu 260 265 Giu Ala Thr Asp Ala Glu Met Thr Ser Ile Leu 275 280 Ser Glu Trp Val Gin Gly Leu Pro Asp Gly Leu 290 295 Ser Gly Ala Asp Ser Leu Ser Gly Gly Gln Arg 305 310 315 Ala Arg Val Leu Leu Ser Asp Ala Pro Ile Leu 325 330 Thr Glu His Leu Asp Thr Ala Gly Ser Ser Glu 340 345 Ala Ser Asp Glu Leu Pro Gly Lys Arg Ala Arg 355 360 Val Arg His Val Arg 370

C

<210> 529 <211> 1368 <212> DNA (213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1345) <223> RXA01339 <400> 529 gagaaggcgg aaccgaagga atcgaagaat atctctccgt gacacatgag ctgtccggtq aataacccga aggaaaaaac PCTJIBOOIOO926 Th r 240 Al a Pro Leu Asp Leu 320 Pro Leu Ile ata gtt gcg cac aat gct gca ggt Ile Val Ala His.Asn Ala Ala Gly gct aag aag aaq tac ctc agc gtt Ala Lys Lys Lys Tyr Leu Ser Val gga acc aac atc ggc gcc ggt gtg Gly Thr Asn Ile Gly Ala Gly Val 45 aca gca cct Thr Aia Pro 15 gcc caa ggc Ala Gin Gly 30 ctc agc ctc Leu Ser Leu gcgttacctc gctttgccgt atg act acc qaa tca Met Thr Thr Glu Ser 1 cag aat gtg tcc tct Gin Asn Val Ser Ser gtt gcc ctt atc tac Val Ala Leu Ile Tyr cca tac gct gcc cgc Pro Tyr Ala Ala Arg 115 163 211 259 WO 01/00805 aac ggt ggt ttc ctc gcc Asn Giy Giy Phe Leu Ala ctg acc acc atc tcc atg Leu Thr Thr Ile Ser Met 75 aag aag cca ctg cag ctt Lys Lys Pro Leu Gin Leu tgg ggc cgc tqg ctg gtg Trp Gly Arg Trp Leu Val 105 gca ctg att gcc tac gca Ala Leu Ile Ala Tyr Ala 120 acc qgc ctg cca cca atc Thr Gly Leu Pro Pro Ile 135 acc ttg atc atg tgg aaa Thr Leu Ile Met Trp Lys 150 155 ttg atc acc act qgc atg Leu Ilie Thr Thr Gly Met 170 aca gtg ctt ggc cct gga Thr Val Leu Giy Pro Gly 185 cca ttc ttc atc gtt ccg Pro Phe Phe Ile Val Pro 200 gcc caa tat gtg gtt cca Ala Gin Tyr Val Vai Pro 215 ccg aag gca gtg cca cgc Pro Lys Ala Vai Pro Arg 230 235 gtc acc ctg gca gct gta Vai Thr Leu Ala Ala Val 250 ggc gtc agt gaa gtt gtc Giy Vai Ser Giu Vai Val 265 gtg gcc tat tac atg gcc Vai Ala Tyr Tyr Met Ala 280 tca ttc atc gcc att gga PCTIBOOIOO926 gcg gga aca 307 Ala Gly Thr ctg gtt gtt gcc ctg ctc att Leu Vai Val Ala Leu Leu Ilie 60 CtC Le u tcc Ser ttc Phe t ca Ser gtg Val1 140 ggc Gly gca Aia att Ile atc Ile gaa.

Glu 220 g cg Al a cc a Pro acc Th r aat Asn ttc tac Tyr gg c Gly att Ile gg a Gly 125 ggc Gly ctg Le u acg Thr tcc Ser atg Met 205 atc Ile atc Ile ttc Phe atc Ile g cc Ala 285 acc atc Ile ctg Leu qcc Ala 110 t cc Ser acc Thr cac His atc Ile gca Al a 190 aac Asn gca Al a atc Ile g cg Ala tcc Ser 270 ttc Phe gcg gcc Ala gca Ala 95 att Ile ggc Gly ctt Le u acg Thr atc Ile 175 gac Asp ctc Leu cga Arg atc Ile gca Ala 255 t gg Trp gca Al a atg gaa Glu 80 gaa Giu gtg Val1 att Ile gga Gly gca Ala 160 atc Ile aac As n gcg Ala gga Gly GI y 240 cta Le u ggc Gly cta Leu cgc gta Val aaa Lys gtc Val1 ttg Leu ttc Phe 145 agc Ser gtg Val ctg Leu gt c Val gtt Val1 225 atg Met ggg Gly ga a Glu ttg Leu aa c tcc Ser t ac Tyr aac Asn ata Ile 130 ttt Ph e ttc Phe ct g Leu atc Ile ttt Ph e 210 aac As n gtc Val1 ctt Le u gca Ala gcc Ala 290 gta ct g Leu ct g Leu agc Ser 115 gg c Gly gtt Val gtg Val tqt Cys gtg Val1 195 acc Thr cct Pro g ca Ala ctg Leu ctc Leu 275 atg Met ct a cg c Arg ggg Gi y 100 gtg Val1 aac Asn tta Leu gag Glu gga Gl y 180 ttc Ph e ttc Phe gcc Al a act Thr gg C Gly 260 gcc Al a ttc Ph e gat acc Thr cag Gin gg t Gly ctc Leu ggc Gly gca Al a 165 t gg Trp cac His ctt Leu acc Thr ggt Gly 245 aca Thr cca Pro act Thr att 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 WO 01/00805 WO 0100805PCTJIBOOIOO926 Ser Phe Ile Ala Ile Giy Phe Thr Ala Met Arg Asn Val Leu Asp Ile 305 295 300 ggc cac tgg cca caa cac gga tgg cag Gly His Trp Pro Gin His Gly Trp Gin 310 315 act gtt ctt cca cca ctg gca att tca Thr Vai Leu Pro Pro Leu Aia Ile Ser 330 gtg gca gca ctg agc tac gcc gga gga Vai Aia Aia Leu Ser Tyr Aia Giy Giy 345 350 atc atc ccc gtg ctg ttg ctg cgc: aac Ile Ilie Pro Val Leu Leu Leu Arg Asn 360 365 gaa cca gtg tgg aaa gcc acc tgg caa Giu Pro Vai Trp Lys Ala Thr Trp Gin 375 380 ctg ttg att gtg gtg tac tcc ctg gog Leu Leu Ile Vai Vai Tyr Ser Leu Aia 390 395 atc gtc gga tta atg cct qcq ggt tg Ile Vai Giy Leu Met Pro Aia Giy Trp 410 tgC '210> 530 <211> 415 <212> PRT <213> Corynebacterium giutamicum <400> 530 Met Thr Thr Glu Ser Ile Val Ala His 1 5 Gin Asn Val Ser Ser Aia Lys Lys Lys 25 Val Ala Leu Ile Tyr Gly Thr Asn Ile Pro Tyr Ala Ala Arg Asn Gly Giy Phe 55 Leu Ile Ala Gly Thr Leu Thr Thr Ile 70 Val Ser Leu Arg Thr Lys Lys Pro Leu Lys Tyr Leu Gly Gin Trp Giy Arg Trp 100 105 cga *tcc gtt gct gtc gga cta Arg Ser Val Ala Vai Giy Leu 320 325 ctt gcg gga tta ggc gga ttc Leu Ala Gly Leu Gly Giy Phe 335 340 ttt gcc gga gca atc atg tcc Phe Ala Gly Ala Ile Met Ser 355 tcc cgc aag agt ggc gac caa Ser Arg Lys Ser Gly Asp Gin 370 gcg cac ccc atc ttc cag atc Ala His Pro Ile Phe Gin Ile 385 ttt gtg tac tcg gtt ctc gcg Phe Vai Tyr Ser Vai Leu Ala 400 405 gca tagtcacttc ctgttgtggc Al a 415 1075 1123 1171 1219 1267 1315 1365 1368 Asn T yr Gly Le u Ser Gin Leu Ala Leu Ala Ala Met Leu Val Ala Ser Gly Leu Leu Ser Ph e Thr Ala Le u Val Ile Leu Ala 110 wo WO 1/00 Val Asn Ser 05s PCT/EBO0100926 Val Gly Ala Leu Ile Ala Tyr Ala Ser Gly Ser Gly Ile Leu Ile 130 Phe Phe 145 Ser Phe Val Leu Leu Ilie Val Phe 210 Val Asn 225 Met Val Gly Leu Glu Ala Leu Ala 290 Asn Val 305 Val Ala Gly Leu Gly Ala Lys Ser 370 Pro Ilie 385 115 Gly Val1 ValI Cys Val1 195 Thr Pro Ala Leu Le u 275 Met Leu Val Gly Ilie 355 Gly Phe As n Leu Giu Gly 180 Phe Phe Al a Thr Gly 260 Ala Phe Asp Gly Gly 340 Met Asp Gin 120 125 Leu Gly Ala 165 Trp His Leu Thr Gly 245 Thr Pro Thr Ile Leu 325 Ph e Ser Gin Ile Thr Thr 150 Le u Th r Pro Ala Pro 230 Val Gly Vai Ser Gly 310 Thr Val Ilie Glu Leu 390 Gly 135 Leu Ile Val1 Phe Gin 215 Lys Thr Val1 Al a Ph e 295 His Val1 Al a Ile Pro 375 Leu Leu Ile Th r Leu Phe 200 Tyr Ala Leu Ser Tyr 280 Ile Trp Leu Ala Pro 360 Val Ile Pro Met Th r Gly 185 Ile Val Val1 Al a G lu 265 Tyr Ala Pro Pro Leu 345 Val1 T rp Val1 Pro T rp Gly 170 Pro Val1 Val Pro Ala 250 Val1 Met Ile Gin Pro 330 Se r Leu Lys Val1 Ile Lys 155 Met Gly Pro Pro Arg 235 Val1 Val1 Al a Gi y His 315 Le u T yr Leu Ala Tyr 395 Val1 140 Gly Ala Ile Ilie Giu 220 Al a Pro Th r As n Phe 300 Gly Al a Ala Leu Th r 380 Ser Gly Leu Thr Ser Met 205 Ile Ile Phe Ile Ala 285 Thr Trp Ile Gly Arg 365 Trp Le u Thr His Ile Ala 190 As n Al a Ile Al a Se r 270 Phe Ala Gin Ser Gly 350 As n Gin Ala Leu Gly Thr Ala 160 Ile Ile 175 Asp Asn Leu Ala Arg Gly Ile Gly 240 Ala Leu 255 Trp Gly Ala Leu Met Arg Arg Ser 320 Leu Ala 335 Phe Ala Ser Arg Ala His Phe Val 400 Tyr Ser Val Leu Ala Ile Val Gly Leu Met Pro Ala Gly Trp Ala 405 410 415 <210> 531 <211> 1452 <212> DNA WO 01/00805 <213> Corynebacterium glutamicum <220> <221> CDS <222> (1429) <223> RXA02527 <400> 531 attggagcaa tcttttaatc aagttgggaa ttccagcgtt PCT/IBOO/00926 gattgtggtt gccatgtggt ttgcccgcac cctgaagcac tcacggatca agcgtggcgg atg ttt ggc ctg ttc Met Phe Gly Leu Phe atc Ile gtg Val ttg Leu ttc Phe cg t Arg gtg Val1 ctc Leu 99 t Gly gat Asp ctg Leu 150 gca Ala gtc Val gcg Ala aca Thr acg Th r ag t S er gga Gly tct Ser gct Ala qgt Gly tcc Ser 135 aat As n atg Met acg Th r att Ile g9 Ala aac Asn ttc Phe aaa Ly s ga t Asp ggc Gly 120 act Thr g tg Val gca Al a at t Ile atc Ile tct Se r 99C Gly atc Ilie gtc Val1 ttg Leu 105 att.

Ile cct Pro gga Gly gga Gly gcg Al a a tq Met ga t Asp att Ile acg Thr 75 gga Gly ttg Le u gc t Ala cca Pro gta Val1 155 ccc Pro att Ile atc Ile gat Asp t gg Trp 60 999 Gly aaa Lys gct Ala ccg Pro act Th r 140 aat As n ttg Leu att Ile gcc Al a ccc Pro 45 ctg Leu ctt Leu atg Met cc t Pro att Ile 125 cg t Arg4 g cg Ala atc Ile ct C Leu gcg Ala 30 ggc Gly att Ile gga Gly ctg Le u gcg Al a 110 atg Met cg C Arq att.

Ile 9C Ala aag Ly s gtg Val1 gcg Ala gt 9 Val1 cy t Arg ggt Gly att Ile aag Lys agg Arq a cg Th r tct S er 175 gtg ValI cca Pro ttg Leu gtg Val a tg Met cga Arg 80 gtg Val1 cca Pro t cg Ser gct Al a tgc Cys 160 ttg Le u gg t GI y 1 atg Met act Thr tat Tyr gcg Ala ata Ile acc Th r tca S er gtg Val ggc Gly 145 9 C9 Ala gct Ala g cg Ala cca Pro 99 t Gly gqc Gly ttc Phe 9 cg Ala tat Tyr gca Al a gc a Ala 130 gC9 Al a a tg Met tcg S er att I le atg Met t tg Le u ctt Leu ctg Leu t tg Le u gg t Gly act T hr 115 t tg Leu ttC Phe ttt Phe c ag Gin gtg Val 195 99 t Gly gt 9 Val att Ile att Ile ttc Phe ttg Leu 100 9C Ala act Thr t tg Leu cta Leu atg Met 180 cct Pro 9C Ala CC9 Pro ggt Gly t cg Ser ttt Phe gCg Ala cga Arg tat Tyr 9 C9 Ala act Th r 165 ga t Asp ggt Gly 115 163 211 259 307 355 403 451 499 547 595 643 691 aat atc acg tg9 aca aac t99 gct Asn Ilie Thr Trp Thr Asn Trp Ala 185 190 WO 01/00805 WO 0100805PCT[IBOO/00926 ctg gtg Leu Val oct gag Pro Glu 215 ctc aag Leu Lys 230 acc ttt Thr Phe gga ato Gly Ile gtg got Val Ala tgg gao Trp Asp 295 tta tcg Leu Ser 310 agt ttg Ser Leu att tao Ile Tyr tct gcg Ser Ala ccc ocg Pro Pro 375 tct toa Ser Ser 390 ttg aac Leu Asn ggc gcg Gly Ala gcg Ala 200 ttg Leu oaa Gin gtt Val tog Se r oa o His 280 acc Th r cag Gin gg t Gly ttc Phe atg Met 360 ttg Leu ctc Leu tac Tyr ctt Leu aag Lys ttg Leu g tg Val1 gca Ala 265 gt g Val a tg Met tao T yr ggo Gly tto Ph e 345 tao T yr atg Met act Thr ato Ile att gtg gtg oog tgg gtg gta Ile Val Val Pro Trp Val Vai 205 tao aag ato tat coa Tyr Lys Ile Tyr Pro 210 gao Asp gg t Gi y ttg Leu 250 aot Thr ot g Leu gtg Vai gga Gly a tg Met 330 agt Ser ttg Leu g og Ala oag Gin a og Thr 410 ac Th r ggg Gly 235 otg Leu aoo Thr a og Thr tgg Trp tto Ph e 315 aao Asn oao His g 00 Ala goo Al a tat Tyr 395 g tg Val1 cct Pro 220 ttt Ph e otg Leu aoo Thr tgg Trp tto Ph e 300 ato Ile t gg Trp tat T yr tto Phe otg Leu 380 tot Ser gg t Gly gag Giu act Thr ctc Leu got Ala gag Glu 285 gog Al a goa Ala gt 0 Val1 tto Phe 0 tg Leu 365 gtg Val gg t Gly gag Giu gt 0 Val1 tat Tyr tg Trp tto Phe 270 gao Asp gtg Val1 t gg T rp gt t ValI ttt Phe 350 ggt Gly ttg Le u gg t Gly t gg Trp aag Lys ggt Gly aca Thr 255 gtt Val ato Ile ota Leu ato Ile got Al a 335 goc Al a got Ala g cg Ala 00 t Pro tgg Trp 415 gt t Val1 aaa Ly s gag Glu 240 gg t Gly gg c Gly att Ile tao T yr tot Ser 320 ttg Leu tog Ser gog Ala tao Tyr tog Ser 400 og g Arg atg Met 225 aag Lys gg 0 Gly gto Val oaa Gin a tg Met 305 gag Glu gtt ValI goa Ala att Ile aoo Th r 385 oca Pro aoo Th r got Al a gtg Val1 gat Asp ato Ile gaa Glu 290 atg Met gt a Val1 gtg Val aoa Thr gog Ala 370 too Ser aoa Thr tog Ser tot Ser otg Leu ttg Leu ato Ile 275 aag Ly s goa Ala att Ile ttg Le u g og Ala 355 att Ilie aat Asn ttg Leu goa Ala ga t Asp g og Al a gto Val 260 ttg Leu aoct Th r aoa Th r got Ala gtg Val1 340 oat His gg t Gly t tg Leu ttt Phe att Ile 420 gag Giu gga Gly 245 ttg Leu ota Leu gog Al a gog Ala too Ser 325 otg Leu att Ile goa Al a tto Ph e gg t Gly 405 got Ala 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 gta tog att aoa ato tgg ttg Val Ser Ile Thr Ile Trp Leu ato ggt ggt ttg tgg atg Ile Gly Gly Leu Trp Met 435 WO 01/00805 aat gtc atc gga ctc tgg taatcgaaaa ttaaaggtaa ggg Asn Val Ile Gly Leu Trp 440 <210> 532 <211> 443 <212> PRT <213> Corynebacterium glutamicum PCTIIBOO/00926 1452 <400> 532 Met Phe Gly Leu Phe Ile Ala Thr Ile 1 Met Th r Tyr Ala Ile Thr Ser Val1 Gly 145 Ala Ala Ala Tyr Met 225 Lys Gly Val Ile Ile Phe Le u 100 Al a Thr Leu Leu Met 180 Pro Tyr Asp Ala 5 Ala Pro Gly Ser Phe Al a Arg T yr Ala Thr 165 Asp Gly Pro Glu Gly 245 Va I.

Leu Phe Arg 70 Val Leu Gly Asp Leu 150 Ala Val1 Le u Pro Leu 230 Thr Thr Thr Ser GI y S er Ala Gly Ser 135 As n Met As n Val1 Giu 215 Lys Ph e Ile 25 Ser Gly Ile Val Leu 105 Ile Pro Gly Gly Thr 185 Leu Lys Leu ValI ValI 10 Gly Ser Th r Ly s Gi y 90 Val1 Met Gly Gin As n 170 Trp Ile Asp Gly Leu 250 Ala Met Asp Ile Thr 75 Gly Leu Al a Pro Val1 155 Pro Thr Val1 Thr Gi y 235 Le u Ile Ile Asp T rp Gly Lys Al a Pro Thr 140 Asn Leu As n Val1 Pro 220 Ph e Leu Ile Ala Pro Leu Leu Met Pro Ile 125 Arg Ala Ile Trp Pro 205 Glu Thr Leu Leu Al a Gly Ile Gly Leu Al a 110 Met Arg Ile Ala Ala 190 Trp ValI Tyr Trp Lys ValI Ala Val1 Arg Gly Ile Lys Arg Th r Ser 175 Val1 Val1 Ly s Gly Thr 255 Pro Leu Val1 Met Ar g Val1 Pro Ser Al a Cys 160 Leu Gly Val Lys Giu 240 Gly Gly Asp Leu Val Leu Gly Ilie Ser Ala Thr Thr Thr Ala Phe Val Gly 260 265 270 WO 01/00805 WO 0100805PCT/IBOO/00926 Val1 Gin Met 305 Giu Val1 Ala Ile Th r 385 Pro Th r Ile Giu 290 Met Val1 Vai Thr Aia 370 Ser Thr Ser Leu Th r Thr Ala Val1 340 His Gly Leu Ph e Ile 420 Leu Val Ala His Val Leu Thr Trp Giu Asp Ile Ilie 280 285 Ala Al a Ser 325 Le u Ile Ala Phe Gly 405 Al a T rp Leu 310 Ser Ile Ser Pro Se r 390 Leu Gly Asp 295 Ser Le u Tyr Al a Pro 375 Ser Asn Al a Thr Gin Gly Ph e Met 360 Leu Leu Tyr ValI Met T yr Gly Ph e 345 Tyr Met Thr Ile Ser 425 Val GI y Met 330 Ser Leu Al a Gin Thr 410 Ile Ph e 300 Ile Trp Tyr Ph e Le u 380 Ser Giy Ile Al a Al a Val1 Phe Leu 365 Val Gly Glu Trp Val1 T rp Val1 Phe 350 Gly Leu Giy Trp Le u 430 Leu Ile Aia 335 Al a Al a Al a Pro T rp 415 Val1 Tyr Ser 320 Le u Ser Al a Tyr Ser 400 Arg Ile Gly Gly Leu Trp Met Asn Val Ile Gly Leu Trp 435 440 z210> 533 <211> 1968 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1945) <223> RXN00298 <400> 533 tttagacaag ttctggttaa aattcttcat gaaggtgaga tctttcagat tcgtagttat ccactgattg gaagaatgag gct atc acg acc gag cct gaa ggg aaa aat aaa Ala Ile Thr Thr Glu Pro Giu Gly Lys Asn Lys 15 gac ccg ttc att ttt tcc att tct gtc ggt ttt Asp Pro Phe Ile Phe Ser lie Ser Val Gly Phe 30 atc gcc aca att gcg cta ggc gag aaa gct cga atctgggaat ttctcggtac atg agc tca aat ata Met Ser Ser Asn Ile 1 aag ggt ctc aaa tca Lys Gly Leu Lys Ser atc gtg gtg ttt gtc Ile Val Val Phe Val aca acc ttt tcc gcg 115 163 211 259 WO 01/00805 WO 0100805PCT/IBOO/00926 Ile Ala Thr Ile Ala Leu Gly Glu Lys Ala Arg Thr att Ile gtC Val1 gg C Gly atc Ile a tg Met atg Met gct Ala 150 ctc Leo

CCC

Pro tcc S er ttt Phe ggt Gly 230 ttg Leu gg t Gly gcc Ala 9CC Ala tcc Ser c99 Arg qtg Val ttt Ph e gct Ala 135 tat T yr cca Pro cg t Arg aca Thr gq t Gly 215 atg Met atc Ile t t9 Leo gt t Val ggC Giy Leu gta Val tgg Trp tgg Trp 120 aat As n act Thr gga Gly cta Leo

CCC

Pro 200 att Ile aac Asn atc Ile ga t Asp gcg Al a tgg Trp gtt Val aaa Lys ttc Ph e 105 gg t Gi y gaa Glu ttc Phe tta Leo tcc Ser 185 99C Gly qct Al a aaa Lys t tg Leu a ag Lys 265 t tg Leu ctc Leu ttc Phe ctt Leu tqt Cys gtt Val gaa Glu tat Tyr tca Ser 170 tct Ser aag Lys gtg Val1 cta Leo atc Ile 250 gg C Gly atg Met tta Leu att Ile 75 gg t Gly atg Met gcc Al a tcc Ser cac His 155 ttg Leu gtg Val ctC Leu tca Ser t gg T rp 235 atc Ile att Ile ttc Ph e g aa Glu 60 ttc Phe ga t Asp ctt Leu gaa Giu atg Met 140 ttc Phe ggqa Gi y ttt Phe atc Ile gta Val1 220 ag c Ser acc Th r aag Lys ttc Phe 45 aat As n ctc Leu gac Asp ttt Phe Ccg Pro 125 agt Ser 99 t Gly t ac Tyr tct Ser gat Asp 205 99 t Gly acc Th r gcg Ala tta Leu atc Ile tta Leu a tg Met ga t Asp gC t Al a 110 att Ile gaa Glu att Ile ttt Phe cg Pro 190 gta Val ctt Leo CCg Pro gtt Val Ctg Leu 270 t tg Leo 999 Gly gg t Gly ga t Asp 95 99C Gly aac As n 9CC Al a cac His att Ile 175 atc Ile ctg Leo 99 t Gly caa Gin gca Al a 255 tcc Ser ttc Ph e t 99 T rp atc Ile 80 ga c Asp 99 t Gly cac His gca Al a atg Met 160 tac Tyr ttg Leo 9CC Ala gtg Val gta Val 240 t gt Cys aac Asn act Th r atg Met ttt Ph e

CCC

Pro gt c Val1 gCg Al a att Ile 145 tgg T rp aaa Lys ggt Gly atc Ile ct 9 Leo 225 tCg Ser att Ile att Ile gt Gi y Thr Phe tat atc Tyr Ile gC9 tcc Ala Ser gag cac Glu His 99t gca Gly Ala 115 ttc aac Phe Asn 130 gtg cag Val Gin gta atc Val Ile Cgt aag Arg Lys aag cac Lys His 195 gta ggc Val Gly 210 caa atc Gin Ile tgg gtt Trp Val tcc gtt Ser Val aat att Asn Ile 275 cca acc Pro Thr Ser 999 Giy Arg acc Thr 100 gtc Val g tg Val gct Al a atg Met cta Leo 180 att Ile acc Thr aat As n cag Gin gc t Ala 260 gca Ala ct C Leo Ala 99 t Gly tat Tyr Cta Leo tta Leo cca Pro ttt Phe gca Al a 165 CC t Pro tat Tyr acg Thr gca Ala ctt Leo 245 tcc S er atg Met aca Th r 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 WO 01/00805 WO 0100805PCT[IB00100926 t tg Leu cct Pro 310 cag Gin cca Pro cgt Arq gtt Val aac As n 390 gca Ala tcC Se r gat Asp caa Gin gtc Val 470 ct 9 Leu tt t Phe gcg Ala ctg Le u 295 aat As n ggC Gly tat Tyr ga a Glu tgg Trp 375 cca Pro gcg Ala gcg Ala t cC Ser aca Thr 455 qca Al a caa Gin gt C ValI gtt Val 280 cgc Arg ctg Leu aaa Lys gtc Val1 ttt Ph e 360 ttc Phe gg t Gly ctt Leu ttt Ph e 9 ca Ala 440 c cg Pro gg t Gly gaa Giu a tg Met cg t Arg 520 ttt Ph e a tg Met t ig Trp ggC Gly 345 atc Ile tct Ser ttc Phe ttt Phe gca Ala 425 gcg Al a act Th r tcc Ser gtg Val atg Met 505 cg t Arg ctc Leu ttt Phe a cg Thr 330 atg Met ggt Gly atc Ile ttg Leu aat As n 410 ctt Leu cta Leu agt S er ttg Leu gtt Val 490 ttt Phe g tg Val gt a Val1 t gg Trp 315 .g tg Val1 ttc Ph e 999 Gi y ttt Ph e acc Thr 395 gtg Val1 gta Val1 gtt Val tac Tyr ctg Leu 475 atc Ile tCt S er cag Gin ga a Glu 300 act Thr ttc Phe gtg Val1 gtt Val1 gg t Gly 380 cag Gin ctg Leu att Ile aac As n cgc Arg 460 atc Ile atc Ile ttg Leu act Thr 285 tcc S er ga c Asp tat Tyr g cg Ala cta Leu 365 cg t Arg cca Pro caa Gin att Ile gat Asp 445 gtg Val1 att Ile gtg Val ctt Le u cgt Arg 525 ttc Phe t ct Ser t gg T rp cg t Arg 350 gct Ala g ca Al a act Thr gag Glu gtg Val1 430 a tg Met atg Met tcc Ser gct Al a aaa Ly s 510 ca 9 Gin gg a Gi y ttc Phe gca Al a 335 att Ile ctg Le u gg C Gly gtt Val1 tat Tyr 415 att Ile ttc Ph e tgg Trp cca Pro ttc Phe 495 ggC Gly tgg Trp atc Ile caa Gin 320 tgg T rp t cg S er c ca Pro atc Ile gtt Val 400 C cg Pro ttC Phe gct Ala gcc Ala t cc S er 480 cca Pro atg Met ga a Glu tat Tyr 305 ga t Asp act Th r cgt Arg gcg Al a gaa Glu 385 gaa Glu ctg Leu ttt Phe acc Thr tgc C ys 465 tct Ser ttc Ph e ag t Ser aag Lys 290 g ca Ala aac Asn att Ile gga Gly atc Ile 370 gtg Val gg t Gly act Th r atc Ile ggt Gly 450 acc Thr ggt Gly ttc Phe ga a Glu act Thr 530 tcc Ser cca Pro tgt Cys cg t Arg 355 ttt Phe gaa Giu ga c Asp gqa Gly acc Thr 435 gca Ala att Ile att Ile ctc Leu ga t Asp 515 gat Asp tgg Trp ggC Gly tgg T rp 340 acc Thr 9gc Gly ctg Leu gtg Val1 att Ile 420 tcc Ser ga a Glu ggg Gly gcc Ala gtg Val 500 g ct Ala a ca Thr atg Met tgg Trp 325 t cg Se r gt c Val1 gta Val1 ag t Ser cca Pro 405 qtc Val at~c Ile aat As n gcg Ala a cg Thr 485 caa Gin gct Ala cca Pro 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 WO 01/00805.

gaa aaa ctt gaa gag cat tcg tcc caa Giu Lys Leu Glu Giu His Ser Ser Gin 535 540 gag ggc aac ccc ttg cca atg cct gcc Glu Gly Asn Pro Leu Pro Met Pro Ala 550 555 aac att gtt atc cca ggc aac gta gtc Asn Ile Val Ile Pro Gly Asn Vai Val 570 gtt ggt gat gtg gtc gac gat cct gag Val Giy Asp Val Vai Asp Asp Pro Glu 585 590 cgt ttt aag atc gtc gag caa act cgg Arg Phe Lys Ile Vai Giu Gin Thr Arg 600 605 gat att taaacgattg cttttcgacg cac Asp Ile 615 <210> 534 <211> 615 <212> PRT <213> Corynebacterium giutamicum <400> 534 Met Ser Ser Asn Ile Ala Ile Thr Thr PCT11B00100926 ic 1747 c ca Pro ctc L eu att Ile 575 gaa Giu ccc Pro tat Tyr gag Giu ctt Leu atg Met 595 gac Asp ga t Asp gac Asp ggg Giy 580 ggg Giy gaa Giu 1795 1843 1891 1939 1968 1 Lys Ile Th r Met Phe Pro Val Ala Ilie 145 Pro Al a Al a Ser Arg Val1 Phe Ala 135 T yr Ph e Thr Gly Leu Val Trp T rp 120 As n Th r GI u Ile Gi y Glu Ph e Asp Leu Giu Met 140 Ph e Gly S er Glu As n Leu Asp Phe Pro 125 Ser Gly Lys Val1 Lys Leu Met Asp Al a 110 Ile Giu Ile As n Gly Ala Gly Gly Asp Gly As n Ala His Lys P he Arg Trp Ile Asp Gly His Ala Met 160 WO 01/00805 WO 0100805PCTAIBOO/00926 Trp Val Lys Arg Gly Lys Ile Vai 210 Leu Gin 225 Ser Trp Ile Ser Ile Asn Gly Pro 290 Tyr Aia 305 Asp Asn Thr Ile Arg Gly Ala Ile 370 Giu Vai 385 Giu Giy Leu Thr Phe Ile Thr Giy 450 Cys Thr 465 Ile Lys His 195 Gi y Ile ValI Vali Ile 275 Thr Se r Pro Cys Arg 355 Phe Giu Asp Gly Thr 435 Ala Ile Met Leu 180 Ile Thr As n Gin Ala 260 Aia Leu Trp Gly Trp 340 Thr Gly Leu Val1 Ile 420 Se r Giu Gly Ala 165 Pro Tyr Thr Al a Leu 245 Ser Met Thr Met Trp 325 Ser Vai Vali Ser Pro 405 Val1 Ile As n Ala Leu Pro Se r Ph e Gly 230 Le u Gi y Al a Leu Pro 310 Gin Pro Arg Val1 Asn 390 Ala Ser Asp Gin Val1 470 Pro Arg Thr Giy 215 Met Ile Le u Val Leu 295 Asn Gly T yr Giu Trp 375 Pro Al a Ala Ser Th r 455 Ala Gly Leu Pro 200 Ile As n I l.e Asp Ala 280 Arg Leu Lys Val1 Ph e 360 Ph e Gly Leu Phe Ala 440 Pro Gly Leu Ser 185 Gly Ala Lys Leu Lys 265 Leu Phe Met Trp Gly 345 Ile Ser Phe Phe Aia 425 Ala Thr Ser Ser 170 Ser Lys Val Leu Ile 250 Giy Met Leu Phe Thr 330 Met Gly Ile Leu Asn 410 Leu Leu Ser Leu Leu Val1 Leu Se r Trp 235 Ile Ile Ph e ValI Trp 315 Val Ph e Giy Ph e Th r 395 Val1 Val1 Val1 Tyr Leu 475 Gly Phe Ile Val 220 Ser Th r Lys Phe Glu 300 Th r Phe Val1 Val Gly 380 Gin Leu Ile Asn Arg 460 Ile Tyr Ser Asp 205 Gly Thr Ala Leu Ile 285 Ser Asp Tyr Ala Leu 365 Arg Pro Gin Ile Asp 445 Val Ile Phe Pro 190 Val Leu Pro ValI Leu 270 Leu Ph e Se r Trp Arg 350 Al a Al a Th r Glu Val 430 Met Met Ser Ile Tyr 175 Ile Leu Leu Ala Gly Vai Gin Val 240 Ala Cys 255 Ser Asn Phe Thr Gly Ile Phe Gin 320 Ala Trp 335 Ile Ser Leu Pro Gly Ile Val Val 400 Tyr Pro 415 Ile Phe Phe Ala Trp Ala Pro Ser 480 WO 01/00805 Ser Gly Ile Ala Thr Leu Gin Giu Val Val Ile 485 490 Phe Phe Leu Vai Gin Phe Val Met Met Phe Ser 500 505 Ser Giu Asp Ala Ala Ala Val Arg Arg Val Gin 515 520 Lys Thr Asp Thr Pro Giu Lys Leu Giu Giu His 530 535 Pro Gly Tyr Asp Asp Giu Gly Asn Pro Leu Pro 545 550 555 His Asp Giu Asp Gly Asn Ile Val Ile Pro Gly 565 570 Gly Asp Leu Gly Val Vai Gly Asp Val Val Asp 580 585 Gin Giu Met Giy Ser Arg Phe Lys Ile Val Giu 595 600 Ser Arg Asp Giu Tyr Asp Ile 610 615 <210> 535 <211> 1968 <212> DNA <213> Corynebacteriun giutamicun <220> <221> CDS <222> (101)..(1945) <223> FRXA00298 <400> 535 tttagacaag ttctggttaa aattcttcat gaaggtgaga tctttcagat tcgtagttat ccactgattg gaaqaatqag PCTAIBOO/00926 Ile Leu Thr S er 540 Met Asn Asp Gin Vai Leu Ar g 525 Ser Pro Val Pro Thr 605 gct Ala gac Asp atc Ile att Ile cc t Pro tcc S er cta Leu tta Leu atctgggaat ttctcgqtac atg agc tca aat ata Met Ser Ser Asn Ilie 1 aag ggt ctc aaa tca Lys Gly Leu Lys Ser atc gtg qtg ttt gtc Ile Val Val Phe Val aca acc ttt tcc gcg Thr Thr Phe Ser Aia atg tat atc ggcj ggt Met Tyr Ile Gly Giy 115 163 211 259 307 gtc ValI 99 C Gly atc Ile a tg Met atg Met gct Ala 150 ctc Le u ccc Pro tcc S er ttt Phe ggt Gly 230 ttg Leu ggt Gly g cc Ala t tq Leu WO 01/00805 tcc ttg gtt Ser Leu Val cgg gta aaa Arg Val Lys gtg tgq ttc Val Trp Phe 105 ttt tqg ggt Phe Trp Gly 120 gct aat gaa Ala Asn Glu 135 tat act ttc Tyr Thr Phe cca gga tta Pro Gly Leu cgt cta tcc Arg Leu Ser 185 aca ccc ggc Thr Pro Gly 200 ggt att gct Gly Ile Ala 215 atg aac aaa Met Asn Lys atc atc ttq Ilie Ile Leu ttq gat aag Leu Asp Lys 265 gtt gcg ttg Val Ala Leu 280 ctg cgc ttt Leu Arg Phe ttc ?he ctt ILeu tg t Cys gt t Val gaa G lu tat T yr t ca Ser 170 tct Ser a ag Lys gtg Val1 cta Leu at c Ile 250 ggc Gly atg Met ctc Leu att Ile 75 ggt Gly a tg Met 9CC Al a tcc Ser cac His 155 ttg Le u gtg Val1 ctc Leu tca Ser tgg Trp 235 atc Ile att Ile ttc Phe gta Val1 ttc Ph e ga t Asp ctt Le u gaa Glu atg Met 140 ttc Ph e gga Gly ttt Phe atc Ile g ta Val1 220 agc S er acc Th r aag Lys ttc Ph e gaa Glu ctc Leu gac Asp ttt Phe C cg Pro 125 agt Ser gg t Gly tac T yr tct S er ga t Asp 205 g9t Gly a cc Thr gcg Ala tta Leu atc Ile 285 tcc Ser atq ggt Met Gly gat gat Asp Asp 95 gct ggc Ala Gly 110 att aac Ile Asn gaa gcc Giu Ala att cac Ile His ttt att Phe Ile 175 ccg atc Pro Ile 190 gta ctg Val Leu ctt ggt Leu Gly ccg caa Pro Gin qtt qca Val Ala 255 ctg tcc Leu Ser 270 ttg ttc Leu Phe ttc gga Phe Gly tct ttc atc Ile 80 gac Asp ggt Gi y cac His gca Ala atg Met 160 tac Tyr ttg Leu gcc Al a gtg Val1 qta Val1 240 tgt Cys aac As n act Thr atc Ile caa ttt Ph e ccc Pro gt c Val gc9 Ala att Ile 145 t gg Trp aaa Lys gg t Gly atc Ile ctg Leu 225 t cg S er att Ile att Ile ggt Gly tat Ty r 305 ga t SCg9 Al a gag Giu Gly ttc Phe 130 g tg Val1 gta ValI cgt Arg a ag Lys gt a Val 210 ca a Gin tgg Trp tcc Ser aat Asn C ca Pro 290 9 ca Ala aac t CC S er cac His gca Al a 115 aac As n cag Gin atc Ile aa g Lys cac His 195 99 C Gly atc Ile gtt Val1 gtt Val att Ile 275 acc Thr t CC Ser cca C99 Arg acc Thr 100 gt c ValI gtg Val1 gct Ala a tg Met cta Leu 180 att Ile a cc Thr aat As n cag Gin gc t Ala 260 9 ca Ala ctc Le u tgg Trp 9gC

PC

tat T yr cta Leu tta Leu C ca Pro ttt Ph e gca Ala 165 cct Pro tat Tyr acg Th r 9 ca Ala ctt Leu 245 t cc Ser atg Met a ca Thr atg Met t 99 TJIBOO/00926 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 295 300 cct aat ctg atg ttt tgg act gac WO 01/00805 WO 0100805PCTIiBOOIOO926 Pro 310 cag Gin cca Pro cg t Arg gtt Val1 aac As n 390 g ca Al a tcC Ser gat Asp caa Gin gtc Val 470 ctg Leu ttt Ph e gcg Al a gaa Glu gag Giu As n ggc Gly tat Tyr gaa Giu t gg T rp 375 cca Pro gcg Al a gcg Al a tcc Ser aca Thr 455 gca Ala caa Gin gt c Val gtt Val1 aaa Lys 535 gg c Gly Leu aaa Lys gtc Val ttt Phe 360 ttc Ph e 99t Gly ctt Leu ttt Ph e gca Ala 440 c cg Pro ggt Giy g aa Giu at g Met cgt Arg 520 ctt Leu aac Asn Met tgg Trp ggc Gly 345 atc Ilie tct Ser ttc Phe ttt Phe gca Ala 425 g cg Al a act Thr tcc Ser gtg Val a tg Met 505 cg t Arg ga a Glu ccc Pro Phe Trp Thr Asp Ser Phe Gin Asp Asn Pro Gly Trp 315 .320 325 acg Th r 330 atg Met ggt Giy atc Ile ttg Leu aat As n 410 ctt Leu ct a Leu ag t Ser t tg Leu gtt Vai 490 ttt Phe gtg Val1 g ag Giu ttg Leu gtg ValI ttc Phe ggg Gly ttt Phe acc Thr 395 gtg Val1 g t a Val1 gt t Val1 ta c Tyr ctg Leu 475 atc Ile tct Ser cag Gin cat His cca Pro ttc Phe gt g Val1 gtt Val1 gg t Gly 380 cag Gin ctg Leu att Ile aac Asn cgc Arg 460 atc.

Ilie atc Ile ttg Le u act Thr tcg Ser 540 atg Met tat Tyr g cg Al a cta Leu 365 cgt Arg cca Pro caa Gin att Ilie gat Asp 445 gtg Val att Ile gtg Val1 ct t L eu cg t Arg 525 tcc Ser cct Pro t gg Trp cgt Arg 350 gct Ala gca Aila act Thr gag Glu gtg Vali 430 atg Met atg Met t cc S er gct Ala aaa Lys 510 c ag Gin caa Gin g cc Ala gca Ala 335 att Ile ctg Leu ggc Giy gtt ValI tat Tyr 415 att Ile ttc Phe tgg Trp cca Pro ttc Phe 495 ggc Gly t gg Trp cca Pro ctc Leu tgg C T rp t cg Ser cca Pro atc Ile gt t Vali 400 ccg Pro ttc Ph e gct Aila gcc Aila t cc Ser 480 cca Pro atg Met gaa Glu gcc Al a gaa Giu act Th r cg t Ar g gcg Aila gaa Glu 385 gaa Giu ctg Leu ttt Phe acc Thr tgc C ys 465 tct Ser ttc Phe agt Ser aag Lys ccg Pro 545 cat His att Ile gga Giy atc Ile 370 gtg Val1 ggt Gly act Thr atc Ile ggt Gly 450 acc Thr gg t Gly ttc Ph e gaa Giu act Thr 530 ggc Gly gat Asp tgt Cys cg t Arg 355 ttt Phe gaa Glu gac Asp gga Gly acc Thr 435 gca Al a att Ilie att Ile ctc Leu gat Asp 515 gat Asp tat Tyr gag Giu tgg Trp 340 acc Thr Giy ctg Leu gtg Vai att Ile 420 tcc S er ga a G lu ggg Gly g cc Ala gt g Vali 500 gct Al a aca Th r gat Asp gac Asp t cg Ser gtc Val gta Vai agt Ser c ca Pro 405 gtc ValI atc Ile aat As n gcg Ala acg Th r 485 caa Gin gct Ala cca Pro gac Asp ggt Giy 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 WO 01/00805 WO 0100805PCT11B00100926 550 555 aac att gtt atc cca ggc aac gta gtc Asn Ile Val Ile Pro Gly Asn Val Vai 570 gtt ggt gat gtg gtc gac gat cct gag Val Gly Asp Vai Val Asp Asp Pro Glu 585 590 cgt ttt aag atc gtc gag caa act cgg Arg Phe Lys Ilie Vai Glu Gin Thr Arg 600 605 gat att taaacgattg cttttcgacg cac Asp Ilie 615 <210> 536 <211> 615 <212> PRT <213> Corynebacterium giutamicum <400> 536 Met Ser Ser Asn Ile Ala Ile Thr Thr 560 att gaa ggt gat ctt ggg Ile Glu Gly Asp Leu Gly 575 580 gaa gcc caa gag atg ggg Giu Ala Gin Giu Met Gly 595 ccc cag tcc agg gac gaa Pro Gin Ser Arg Asp Giu 610 1843 1891 1939 1968 1 Lys Ile Thr Met Phe Pro Vai Al a Ile 145 Trp Lys Gly Val Th r Tyr Aila Gi u Gly Ph e 130 Val1 Val1 Arg Leu Val1 Phe Ile S er His Ala 115 Asn.

Gin Ile Lys Lys Phe Ser Giy Arg Thr 100 Vai Val1 Ala Met Leu 180 5 Ser ValI Aia Gly Tyr Leu Leu Pro Phe Ala 165 Pro Asp Ile Ile Vali 70 Gly Ile Met Met Aia 150 Leu Pro Ph e Th r 40 Gly Leu ValI Trp Trp 120 Asn Th r Gl y Le u Ile 25 I le Trp Val1 Lys Phe .105 Gly Glu Phe Leu Ser 185 Giu 10 Ph e Al a Leu Phe Leu 90 Cys Vali Giu Tyr Se r 170 Ser Pro Ser Le u Le u Ile 75 Giy Met Ala Ser His 155 Leu Val1 Glu Ile Giy Giu Phe Asp Leu Giu Met 140 Ph e Giy Ph e Gly Ser Giu As n Leu Asp Ph e Pro 125 Ser Gly Tyr Ser Lys Val Lys Leu Met Asp Aia 110 Ile Giu Ile Phe Pro 190 Asn Lys Gly Phe Ala Arg Gly Trp Giy Ilie Asp Asp Gly Gly Asn His Ala Ala His Met 160 Ile Tyr 175 Ile Leu WO 01/00805 PCT/IB00/00926 Gly Lys His Ile Tyr Ser Thr Pro Gly Lys Leu Ile Asp Val Leu Ala 195 200 205 Ile Val Gly Thr Thr Phe Gly Ile Ala Val Ser Val Gly Leu Gly Val 210 215 220 Leu Gin Ile Asn Ala Gly Met Asn Lys Leu Trp Ser Thr Pro Gin Val 225 230 235 240 Ser Trp Val Gin Leu Leu Ile Ile Leu Ile Ile Thr Ala Val Ala Cys 245 250 255 Ile Ser Val Ala Ser Gly Leu Asp Lys Gly Ile Lys Leu Leu Ser Asn 260 265 270 Ile Asn Ile Ala Met Ala Val Ala Leu Met Phe Phe Ile Leu Phe Thr 275 280 285 Gly Pro Thr Leu Thr Leu Leu Arg Phe Leu Val Glu Ser Phe Gly Ile 290 295 300 Tyr Ala Ser Trp Met Pro Asn Leu Met Phe Trp Thr Asp Ser Phe Gin 305 310 315 320 Asp Asn Pro Gly Trp Gin Gly Lys Trp Thr Val Phe Tyr Trp Ala Trp 325 330 335 Thr Ile Cys Trp Ser Pro Tyr Val Gly Met Phe Val Ala Arg Ile Ser 340 345 350 Arg Gly Arg Thr Val Arg Glu Phe Ile Gly Gly Val Leu Ala Leu Pro 355 360 365 Ala Ile Phe Gly Val Val Trp Phe Ser Ile Phe Gly Arg Ala Gly Ile 370 375 380 Glu Val Glu Leu Ser Asn Pro Gly Phe Leu Thr Gin Pro Thr Val Val 385 390 395 400 Glu Gly Asp Val Pro Ala Ala Leu Phe Asn Val Leu Gin Glu Tyr Pro 405 410 415 Leu Thr Gly Ile Val Ser Ala Phe Ala Leu Val Ile Ile Val Ile Phe 420 425 430 Phe Ile Thr Ser Ile Asp Ser Ala Ala Leu Val Asn Asp Met Phe Ala 435 440 445 Thr Gly Ala Glu Asn Gin Thr Pro Thr Ser Tyr Arg Val Met Trp Ala 450 455 460 Cys Thr Ile Gly Ala Val Ala Gly Ser Leu Leu Ile Ile Ser Pro Ser 465 470 475 480 Ser Gly Ile Ala Thr Leu Gin Glu Val Val Ile Ile Val Ala Phe Pro 485 490 495 Phe Phe Leu Val Gin Phe Val Met Met Phe Ser Leu Leu Lys Gly Met 500 505 510 Ser Glu Asp Ala Ala Ala Val Arg Arg Val Gin Thr Arg Gin Trp Glu WO 01/00805 515 520 Lys Thr Asp Thr Pro Giu Lys Leu Glu Giu His 530 535 Pro Gly Tyr Asp Asp Giu Giy Asn Pro Leu Pro 545 550 555 His Asp Giu Asp Giy Asn Ile Vai Ile Pro Giy 565 570 Gly Asp Leu Giy Val Vai Gly Asp Val Val Asp 580 585 Gin Giu Met Giy Ser Arq Phe Lys Ilie Vai Giu 595 600 Ser Arg Asp Giu Tyr Asp Ile 610 615 <210> 537 <211> 576 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (553) <223> RXA00596 <400> 537 ccgccaccga cggcctcttg aacaccgatg cataccaaca ccatcggagt gccaagccct agctaccagg gaggaaacta PCT/IBOO/00926 Ser 540 Met Asn Asp Gin Gin Pro Ala Aia Leu Giu 560 Vai Ile Giu 575 Giu Giu Ala 590 Arg Pro Gin aaa L~ys g tg Val1 atc Ile t ca Ser g aa G iu ttt Phe ttc Phe atc Ile cgc Arg a cg Thr ccc Pro gg t Gly cca Pro gc t Ala cca Pro atc Ile aag Lys ga t Asp tgg Trp q cg Aia ctt Leu acc Thr

CCC

Pro cca Pro Ct 9 Leu gta Val cga Arg g cg Aila 99 C Gly 75 gta Val atc Ile aaa Lys gt 9 Vali act Thr 60 gac Asp gaa Glu 9 9C Giy aag Lys acc Thr cc t Pro ccc Pro gtt Val gctgtgCtC ggtgaaaatg atg ctt aac gCC ctg Met Leu Asn Aia Leu 1 ctc tct ggc ttc agc Leu Ser Giy Phe Ser ttc aac CCC gtt gtt Phe Asn Pro Vai Vai cag atc gca 9CC ctg Gin Ile Ala Ala Leu tcc ctt ggc cta cgC Ser Leu Gly Leu Arg ctg atc caa gca gtg Leu Ile Gin Ala Val atc gcc gat atq gaa Ile Ala Asp Met Giu 100 115 163 211 259 307 355 403 WO 01/00805 WO 0100805PCT/IBOO/00926 caa cgc ctc gtc cct tcg qtc gct tca att gac cac ggc gtc cca ggc Gin Arg Leu Val Pro Ser Val Ala Ser Ile Asp His Gly Val Pro Gly 105 110 115 caa ggc cct tac aag gag atc cgc ccc agc gat gct gag tgg cca agt Gin Gly Pro Tyr Lys Giu Ilie Arg Pro Ser Asp Ala Giu Trp Pro Ser 120 125 130 cgc gag atc gct gac acc gcc caa aac acc gtc agc caa gac aag agg Arq Giu Ile Ala Asp Thr Ala Gin Asn Thr Val Ser Gin Asp Lys Arg 135 140 145 gag ttt taaaacaaca tgactgcttt tgg Giu Phe 150 <210> 538 <211> 151 <212> PRT <213> Corynebacteriun giutamicum <z400> 538 Met Leu Asn Ala Leu Lys Phe Ilie Pro Trp Leu le Gly Gin Ile Phe 451 499 547 576 1 Le u Phe Gin Ser Leu Ile His Al a Ser 145 Gly Pro Aia Gly Gin Asp ValI 115 Trp Asp Phe Val1 Ala Leu Ala Met 100 Pro Pro Lys 5 S er Val1 Leu Arg Val1 Giu Gi y Ser Arg Val1 Ile S er Glu 70 Phe Gin Gin Arg Giu 150 Ile Arg Thr Pro Gly Arg Gly Giu 135 Phe Thr T yr 40 Cys Arg Ser Le u Pro 120 Ile Ala 25 Pro Ile Lys Asp Val1 105 Tyr Al a 10 Ala Le u Thr Pro Pro 90 Pro Lys Asp Val Arg Ala Gly 75 Val1 S er Giu Thr Lys Val1 Th r Asp Giu Val1 Ile Al a 140 Lys Asp Thr Thr Pro Ser Pro Thr Val Phe Ala Ser 110 A rg Pro 125 Gin Asn Thr Asp Th r Ile Giu Ilie Se r Thr Gi y Ph e Leu Leu S er Asp Asp Val <210> 539 <211> 750 <212> DNA <213> Corynebacterium giutamicum <220> WO 01/00805 <221> CDS <222> (727) <223> RXA02364 <400> 539 gcgaaagtat ctaaggtcgt ttgattggtt tactaccccc catcgagcac caaatgagcg tgatccggga tcaggatctg PCTIBOO/00926 gaagtggggg ttattggcag caa Gin caa Gin gct Ala etc Ile Giy aat As n t ac Tyr cga Arg ctg Leu aca Th r 150 ttc Ph e aaa Lys gtg Val1 ag t Ser ggc Gi y ga c Asp cg c Ar g gag Glu aac As n aec As n cac His gee Glu 135 gee Glu gt t Vali gac Asp cgc Arg ctt Leu acc Thr ctg Leu etg Met gte Val gag Giu agc S er get Asp 120 gee Giu act Thr gag Giu gee Giu gcc Ala gcg Ale cge Arg gct Ala att Ilie tat T yr etc Ile eac As n 105 c tg Leu t gg T rp get Asp g tg Val1 ttc Phe 185 gct Ala gee Giu eat Asn gga Gi y gee Glu ecc Th r agt Ser acc Thr ctt Leu gac Asp gcc Al a get Asp 170 ttg Le u qat Asp t cg Ser ctg Leu aeg L ys ctc Leu gcg Ale 75 tac Tyr eat Asn gec Asp gcc Ale gtt Val1 155 gcg Ala acc Th r act T hr ga c Asp t ec Tyr aeg L ys a tg Met 60 etg Met gt t Val cac His gcc Ala gcg Ala 140 ett Ile caa Gin tca Se r tca Ser eat As n acc Thr att Ile 45 gg t Gi y ceg Gin acc Thr ctt Leu atg Met 125 etg Met gae Giu gca Ala gee Giu gga Gly att Ile act Thr 30 cg c Arg ggc Gi y tct Ser caa Gin gt c Val1 110 agc Ser act Thr aeg Lys ttt Ph e ttc Phe 190 gg t Gly t ct S er 15 get Asp gtc Val t cg Ser gg t Gly eec Asn 95 ggt Gly gag Glu gag Giu gcg Ale acc Thr 175 cag Gin g cg Ala gt c Val1 gcg Al a ca g Gin gca Ala gtg Val 80 cac His tt g Leu cc a Pro ca c His aaa Lys 160 get Asp cg t Ar g gca Ala etg gag Met Glu 1 ett ggt Ile Gly ccg gtg Pro Val gee tcc Giu Ser acc ccg Thr Pro ctg get Leu Asp ttc gag Phe Glu get tec Asp Tyr gac cgt Asp Arg 130 ecg gat' Thr Asp 145 gct ggg Ai1, Gly gce ctg Ala Leu gee ctc Glu Leu gg a Gly aa Lys gcc Ale ttg Leu gg c Gly gt t Val1 atg Met 115 gee Giu c tgC Leu g ga Gly gct Ala tec Tyr 195 cca ctg ttc Pro Leu Phe ttt Phe agea Arg etg Met act Thr gcg Ala gct Ala 100 gtc Val1 t tg Leu tgg Trp gc9 Ale ccc Pro 180 gee acc Thr ccc Pro cac His tac Tyr ga a Glu cgc Arg atg Met ttc Phe eec As n gag Glu 165 etc Ile gca 115 163 211 259 307 355 403 451 499 547 595 643 691 Glu Ala tca Ser tgatgaattt WO 01/00805 PCTIIBOO/00926 200 205 taagtccatc gtg 750 <210> 540 <211> 209 <212> PRT (213> Corynebacterium giutamicum <400> 540 Met Giu Pro Leu Phe Gin Ser Leu Ala Giu Ser Asp Asn Ile Ser Val 1 Ile Pro Glu Th r Leu Phe Asp Asp Thr 145 Al a Ala Gi y Val Ser Pro Asp Giu Tyr Arg 130 Asp Gly Leu Gly Asp Arg 55 Giu As n As n His Giu 135 Glu ValI Asp Th r Leu Met Val Giu Se r Asp 120 Giu Thr Giu Giu As n Gly Glu Th r Se r Thr Le u Asp Ala Asp 170 Le u Tyr Lys Met Met Val His Ala Ala 140 Ile Gin Se r Thr Ile Gly Gin Th r Leu Met 125 Met Giu Aia Giu Thr Arg Gly Ser Gin Val1 110 Ser Th r Lys Phe Phe 190 Asp Val Ser Gly Asn Gly Giu Giu Aia Thr 175 Gin Ala Gin Aia Val His Leu Pro His Lys 160 Asp Arg Giu Leu Tyr 195 Ser Giu Aia Vai Arq Ala Asp Thr Ser Gly Ala Ala (210> 541 <211> 888 <212> DNA (213> Corynebacterium giutamicum <220> (221> CDS WO 01/00805 <222> (101)..(865) <223> RXN01411 <400> 541 cttatcgacg tccccatccc cctcgccaat gcttcggcga PCTIIBOO/00926 ggggttctat ttattgtgtg tgctagcctt ttcgcaatcg ttcagcccgc cccgacgtca atg ttg gga gtg ggc Met Leu Gly Val Gly tgg Trp tgg T rp ccc Pro t tg Leu ttc Phe ggC Gly att Ile aac Asn gCC Ala cag Gin 150 gt a Val ggC Gly cg c Arg atc Ile qa a Giu Arq acc Thr a cg Th r gt g Val atc Ile tac Tyr 135 gt a Val1 atg Met att Ile att Ile cg c Arg gct Ala gt a Vali a cg T hr tta Leu 9CC Ala atg Met 120 atc Ilie atg Met atg Met acc Th r cc a Pro aCC Th r cct Pro ggt Gly t ac Tyr gtg Val1 cca Pro 105 gct Ala att I le ct t Leu

C

Ala tac Tyr 185 ttc Phe ggt Gly att Ile Gly atg Met ctg Leu ttt Phe ttc Phe gca Ala gga G 1y gag Giu 170 aac As n c tg Leu gcc Ala a ag Lys ttt Phe acc Th r 75 gct Ala gtt Val gct Ala ggt Gly atc Ile 155 gt c Val1 gt c ValI atg Met cag Gin cag Gin atc Ilie 60 act Thr gga Gly g gc Gly acc Th r caa Gin 140 99C Gi y ttc Phe act Thr gcc Al a ga a Giu 9 ca Al a 45 t ct Ser ttc Phe aac Asn cg c Arg tta Leu 125 ggt Gly gcg Ala caa Gin tac Tyr gtg ValI aat As n 30 t tg Leu tgc C ys ctg Leu atc Ile gca Ala 110 agc Se r act Thr gt t ValI gag Giu gca Ala 190 cca Pro 15 gt a Val cg t Arg acc Thr cgc Arg atc Ile 95 att Ile aca Th r ttg Leu acc Thr gtc Val 175 atc Ile acc Thr cta Leu cgc Arg act Thr 9gt Gly agc Ser 80 gct Ala gat Asp gt a Val1 act Thr gct Al a 160 acc Thr ttc Ph e ggc Gi y 1 ggg Gly ccc Pro gag Glu ctg Le u acc Thr ctc Leu aaa Lys att Ile g ct Ala 145 aac As n cgc Arg 99 c Giy agc Se r ctt Leu 9 ca Ala t gg T rp agc Ser gt c Val1 agc Ser ttc Phe atg Met 130 tct Se r tgc Cys 99 t Gly 9gc Gly C C9 Pro 210 atc Ile tcc Ser aa g Ly s ttc Phe 99 a Gly a tg Met ccc Pro 115 gcg Ala ttg Leu gtt Val1 act Thr t cg Ser 195 Ct 9 Leu ggC Gly ga a Glu atq Met tac Tyr Ctg Leu gca.

Ala 100 CgC Arg atc Ile att Ile a cc Thr tcC Ser 180 gct Ala 9C Al a tgg Trp Cgc Ar g atg Met att Ile gag Glu gca Ala Cg99 Arg CC9 Pro gcg Ala tca S er 165 9C Ala cca Pro cct Pro 115 163 211 259 307 355 403 451 499 547 595 643 691 739 ttt atc tcc acc gca ttg gtc tcc tgg Phe Ilie Ser Thr Ala Leu Val Ser Trp WO 01/00805 PCT11B;00/00926 gcg gta tac atg a *tc atc att gcg ctc ttc gcc ttc acc gcg tcc cgc 787 Ala Vai Tyr Met Ile Ile Ile Ala Leu Phe Ala Phe Thr Ala Ser Arg 215 220 225 ttc att cct gaa acc tcc cca gtt ttt gtc acc gca acc ccg gcc att 835 Phe Ilie Pro Glu Thr Ser Pro Val Phe Val Thr Ala Thr Pro Ala Ile 230 235 240 245 aag gca cca aag gtg ctg gtc aac ccg ggt taaaccacgc ttttcgacga 885 Lys Ala Pro Lys Val Leu Val Asn Pro Gly 250 255 aaa 888 <210> 542 <211> 255 (212> PRT <213> Corynebacterium giutamicun <400> 542 Met Leu Gly Val Leu Ala Trp Se r Vai Se r Phe Met 130 Se r C ys Gly Gly Gi u Met Tyr Leu Ala 100 Arg Ile Ile Thr Se r 180 Gly Trp Arg Met Ile Giu Ala Arg Pro Ala Ser 165 Ala Trp Arg Ile Pro T rp Pro Leu Phe Gly Ile Asn Ala Gin 150 Vai Giy Ile Glu Arg 55 Thr Th r Val1 Ilie Tyr 135 Val Met Ile Arg Ala Val1 Thr Leu Ala Met 120 Ile Met Met Th r Thr 25 Pro Gly Tyr Val Pro 105 Ala Ile Le u Ala Tyr 185 Ph e 10 Gly Ile Gly Met Le u 90 Phe Phe Ala Gly Giu 170 Asn Leu Ala Lys Phe Thr 75 Ala ValI Ala Gly Ile 155 Val1 Val1 Met Gin Gin Ile Th r Gly Gly Th r Gin 140 Gly Phe Thr Ala Glu Ala Se r Phe Asn Arg Le u 125 Gly Al a Gin Tyr Val1 Asn Le u Cys Leu Ile Ala 110 Ser Th r Val1 Giu Ala 190 Pro ValI Arg Thr Arg Ile Ile Thr Le u Thr Val1 175 Ile Le u Arg Thr Gly Se r Ala Asp ValI Thr Ala 160 Thr Phe Gly Gly Ser Ala Pro Phe Ilie Ser Thr Ala Leu Val Ser Trp Thr Gly WO 01/00805 Ser Pro Leu Ala Pro 210 Phe Thr Ala Ser Arg 225 Ala Thr Pro Ala Ile 245 PCTABOO/00926 Ala Val Tyr Met Ile Ile Ile Ala Leu Phe Ala 215 220 Phe Ile Pro Glu Thr Ser Pro Val Phe Val Thr 230 235 240 Lys Ala Pro Lys Val Leu Val Asn Pro Giy 250 255 <210> 543 <211> 1058 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(1035) <223> RXN00960 <400> 543 atg Met

I

ggt Gly aaa Ly s cag Gin gca Ala t ca Se r aag Lys gg C Gly t CC Ser gct Al a ctg Leu ga c Asp ccc Pro ctg Leu gac Asp ac c Tb r atc Ilie gcc Al a 130 C9g Arg atc Ile cac His tac Tyr ctq Leo acc Thr atc Ile ttC Phe 115 ttt Phe cat His qcc Ala caa Gin ttC Phe ct g Le u ct g Leu acc Th r 100 ct C Leu gg C Gly tgt tgc Cys Cys 5 tac gqa Tyr Gly agc gtc Ser Val aac atc Asn Ile gca ttc Ala Phe 70 ttc aag Phe Lys gcc ttt Ala Phe ggc atg Gly Met aag gta Lys Val ag c Ser qc a Ala acc Tb r gag Gbu 55 tgc Cys gt a Val gtC Val 9gc Gly ct g Leu 135 aat Asn tcc Se r gaa Glu 40 atg Met ct C Leu acc Thr atc Ile a tg Met 120 att Ile cg C Arg caa Gin 25 atc Ilie cca Pro gg c Gly cgc Arg cca Pro 105 aac Asn ctc Leu tac Tyr 10 g cg Ala qac Asp ca Pro ctg Leu gaa Giu 90 ctg Leu ggt Gly gc C Ala g cg Ala Ct C Leu ctt Leo cca Pro 99C Gly 75 ctc Leu ctg Leu ggc Gi y gt c Val1 tC Ser tac Tyr ga t Asp ttt Ph e atq Met gag Glu cca Pro ctc Leu gtg Val1 140 acc Thr cca Pro gca Ala ga a Glu gct Ala cgc Arg ctC Leu ctg Leu 125 ga Gly gtC Val tgg Trp ggt Gly gtg Val gta Val1 gt a Val1 ttc Phe 110 gag Glu acc Th r ttc Phe ctg Leo gcc Aia atg Met att Ile gtC Val atc Ile atc Ile ctg Leo t CC Ser ctg Leo ctc Leo acc Thr aaa Lys atg Met ttC Phe atg Met ct C Leu 48 96 144 192 240 288 336 384 432 ttC cta gcc Phe Leu Ala 145 atC cag ttc att atc gct ggt gca gta tcC aag aag aac Ilie Gin Phe Ile Ile Ala Giy Ala Val Ser Lys Lys Asn WO 01/00805 WO 0100805PCT[IBOO/00926 cca Pro 99c Gly ctg Leu tgC C ys acc Thr 225 t cc Se r ggC Gly atg Met atc Ile g ca Ala 305 act Thr tgg Trp act Thr aaa Lys gcc Ala 210 ttc Phe atc Ile gt t ValI ctc Le u gcg Ala 290 atc Ilie tcC Ser aaa Lys tcc Ser aac Asn 195 acc Thr gct Ala g ga Gly ccc Pro gga Gly 275 att Ile gca Ala cca Pro ct g Leu tct Ser 180 ga t Asp atc Ile gt t Val1 ttc Phe gg c Gi y 260 ttc Ph e gac Asp gt C Va 1 ga t Asp ttc Phe 165 tca Ser gtt Val1 cac His gtC Val ct C Leu 245 gga Gly aac As n tcC Ser atc Ile gaa Glu 325 aaa Lys qcg Ala ga t Asp ct a Leu ttC Phe 230 ctc Leu gc c Al a acc Th r ttc Ph e gt g Val 310 atc Ile aac As n g ca Ala gtc Val1 q ct Ala 215 a tg Met a tg Met atc Ile gaa Glu ggc Gly 295 aac As n gaa Glu atg Met a cc Th r aac As n 200 gga Gly tac T yr ctg Leu atg Met caa Gin 280 acc Thr aaa Lys ga a Glu Ctc: cct Leu Pro 170 atc cca Ile Pro 185 gtc gca Val Ala tcg atq Ser Met gac: atg Asp Met ggc atc Gly Ile 250 gca gca Ala Ala 265 gtc gcc Val Ala gca gca Ala Ala ttc gC Phe Ala gac gac Asp Asp 330 gca Ala gtg Val1 ggc Gly atg Met ga a Gi u 235 acc Thr acc Th r ctc Le u aac As n aag Ly s 315 cq C tac Tyr acc Thr ttt Ph e aag Lys 220 gta Val1 atg Met ggc Gly atg Met gtc Val 300 9gc Gly gtt ttc Phe tac Tyr gtt Val1 205 atc: Ile Gly atc Ile atg Met atc Ile 285 acc Thr cag Gin 9C act Th r ca g Gin 190 gtC Val1 gg c Gly gtc ValI g cc Ala ctg Leu 270 gcc Ala ggc Gly ctg Leu ttC gca ctg Ala Leu 175 cag acc Gin Thr Cca Ctg Pro Leu ctc ttc Leu Phe ggc ctc Gly Leu 240 gca cca Ala Pro 255 9CC tcc Ala Ser gct tac Ala Tyr gac ggc Asp Gly cac acc His Thr 320 gac atc Asp Ile 335 528 576 624 672 720 '768 816 864 912 960 1008 1055 1058 Arg Val Ala Phe act cca tcg gat gtg gaa cat cac aag tagaaacccg cattttctgt Thr Pro Ser Asp Val Glu His His Lys 340 345 agt <210> 544 <211> 345 <212> PRT <213> Corynebacterium glutamicum <400> 544 Met Ala Arg His Cys Cys Ser Asn Arg Tyr Ala Ser Thr Val Phe Ser 1 5 10 Gly Leu Ilie Ala Tyr Gly Ala Ser Gin Ala Leu Tyr Pro Trp Leu Leu WO 01/00805 WO 0100805PCTIIBOO/00926 Lys Gin Ala Ser Lys Gly Ser Phe 145 Pro Gly Leu Cys Th r 225 Ser Gly Met Ile Ala 305 Thr Thr Asp Pro Le u Asp Th r Ile Ala 130 Leu Trp Thr Lys Ala 210 Phe Ile Val Leu Ala 290 Ile Se r Pro His Tyr Leu Thr Ile Phe 115 Phe Ala Lys Ser As n 195 Th r Ala Gly Pro Giy 275 Ile Ala Pro Ser Gin Phe Leu Leu Th r 100 Le u Giy Ile Leu Ser 180 Asp Ile Val1 Phe Gly 260 Ph e Asp Val1 Asp Asp 340 Ser As n Ala Phe Ala Giy Lys Gin Ph e 165 Ser Val1 His Vali Leu 245 Gly Asn S er Ile Glu 325 Val1 ValI Ile Ph e 70 Lys Phe Met Val1 Phe 150 Lys Ala Asp Leu Phe 230 Leu Al a Th r Ph e Val 310 Ile Glu Th r Giu 55 Cys ValI Val Gly Leu 135 Ile As n Ala Val Ala 215 Met Met Ile Glu Gly 295 Asn Glu His Glu Met Leu Thr Ile Met 120 Ile Ile Met Thr As n 200 Gly Tyr Leu Met Gin 280 Th r Lys Giu His Ile Pro Gly Arg Pro 105 As n Leu Al a Leu Ile 185 Val Se r Asp Gly Ala 265 Val1 Ala Phe Asp L ys 345 Asp Pro Leu Giu 90 Leu Gly Ala Gly Pro 170 Pro Ala Met Met Ile 250 Al a Ala Al a Ala Asp 330 Leu Pro Gly 75 Leu Le u Gly Val1 Al a 155 Ala Val1 Gly Met Giu 235 Thr Thr Leu Asn Lys 315 Arg Asp Ph e Met Glu Pro Leu Val1 140 Val Tyr Thr Ph e Lys 220 Val Met Gly Met Val 300 Gly Val1 Al a Giu Ala Arg Leu Le u 125 Gly S er Phe Tyr Val 205 Ile Gly Ile Met Ile 285 Th r Gin Ala Gly ValI Val1 Val Ph e 110 Giu Thr Lys Thr Gin 190 Val1 Giy Val1 Ala Leu 270 Ala Gi y Leu Ph e Ala Met Ile Val Ile Ile Le u Lys Ala 175 Gin Pro Leu Gly Ala 255 Ala Ala Asp His Asp 335 Leu Thr Lys Met Phe Met Leu As n 160 Leu Thr Leu Phe Leu 240 Pro Ser Tyr Gly Thr 320 Ile WO 01/00805 PCT1B00100926 <210> 545 <211> 1118 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(1095) <223> RXN02447 <400> 545 aca gta gtt ccg gtg tae etc gct gaa etc gca cca eta gaa ate cgc 48 Thr Val Val Pro Val Tyr Leu Ala Glu Leu Ala Pro Leu Glu Ile Arg 1 5 10 ggc tee ctg ace ggc ega aac gag ctt gct ate gte ace ggc cag ctg 96 Gly Ser Leu Thr Gly Arg Asn Giu Leu Ala Ile Val Thr Gly Gin Leu 25 ctt gcc ttc gtg atc aac gcg ctt ate gcc gtc acc cta cac gga gtt 144 Leu Ala Phe Val Ile Asn Ala Leu Ile Ala Val Thr Leu His Gly Val 40 att gat gga ate tgg cge ate atg ttc gec gte tgt gcc ctc cet gee 192 Ile Asp Gly Ile Trp Arg Ile Met Phe Ala Val Cys Ala Leu Pro Ala 55 gtc gcc ctc ttc ctc ggc atg ctg cgg atg ccg gaa tea cea cge tgg 240 Val Ala Leu Phe Leu Gly Met Leu Arg Met Pro Giu Ser Pro Arg Trp 70 75 etg gte aac eag qgg cgt tac gac gac gee cgc cgc gtc atg gag acc 288 Leu Val Asn Gin Gly Arg Tyr Asp Asp Ala Arg Arg Val Met Giu Thr 90 gte egt ace ect gag cgt gcg aaa gee gaa atg gat gaa ate ate geg 336 Val Arg Thr Pro Glu Arg Ala Lys Ala Giu Met Asp Giu Ile Ile Ala 100 105 110 gtg cac tet gaa aae aat gcg gca ctt ect ggt gtt aag eag tet teg 384 Val His Ser Giu Asn Asn Ala Ala Leu Pro Gly Val Lys Gin Ser Ser 115 120 125 ggc cag get tea ggc cag gtt tet age aag cac ace eac atg tee ate 432 Gly Gin Ala Ser Gly Gin Val Ser Ser Lys His Thr His Met Ser Ile 130 135 140 ggc gaa gte etc age aac aaa tgg ctg gtt cgt ctg etc ate gee ggc 480 Gly Giu Val Leu Ser Asn Lys Trp Leu Val Arg Leu Leu Ile Ala Gly 145 150 155 160 ate ggt gtt gca gtt gee cag cag etc ace gge ate aac gee atc atg 528 Ile Gly Val Ala Val Ala Gin Gin Leu Thr Gly Ile Asn Ala Ile Met 165 170 175 tac tac gga ace ege gte etc gag gaa tee gge atg age gea gaa atg 576 Tyr Tyr Gly Thr Arg Vai Leu Glu Giu Ser Gly Met Ser Ala Glu Met 180 185 190 WO 01/00805 PCTIBOOIOO926 git gcc gtc ato ggt gga 624 Val Ala Val Ile Gly Gly 205 got Ala 0tg Leu atc Ile 225 cC Ala a tg Met gtt Val ggt Gly gt C Val1 305 ttC Phe gto Val gat Asp g tg Val1 ato Ilie 210 atc Ile Giy ato Ilie g ca Ala atc Ilie 290 ota Leu too Ser acc Thr cac His gtt Val 195 gca Al a ggo Giy act Thr ott Leu gtg Val 275 ggC Gly go g Ala tto Phe aag Lys gca Al a goo aao att got Ala Asn Ile Ala otg ogo aao atg Leu Arg Asn Met 215 ctg toa otg aoo Leu Ser Leu Thr 230 oto ott oca gaa Leu Leu Pro Glu 245 gtt gtt ggg ttC Val Val Gly Phe 260 tgg gtg tgg otg Trp Val Trp Leu aco ggt att tog Thr Gly Ile Ser 295 ttg tto tto oca Leu Phe Phe Pro 310 Ott ato tto goa Leu Ile Phe Ala 325 ttt gtt oct gaa Phe Val Pro Giu 340 goa tto aco 990 Ala Phe Thr Gly ggt 900 Gly Ala gac Asp a co Thr ggt Gly 9 tg Val1 g9 Al a 280 gta Val gca Ala gto ValI acc Thr 090 Arg aco Thr aao As n ct 0 Leu 265 ga a Giu ttc Phe ctg Leu gto Val1 ogt Arg 345 otg Leu tto Phe too Ser 250 too Ser ato Ile tgC C ys gt 0 Val1 gga Gi y 330 990 Gl y gat Asp cac His 235 att Ile atg Met ttc Phe 99 t Gly too Ser 315 gto Val1 090 Arg 090 Arg 220 Ott Leu oga Arg oag Gin oca Pro tgg Trp 300 990 Gly att Ile tca Ser 090 Arg t tg Leu cca Pro act Th r gtC Val1 285 990 Gly gtg Val1 900 Ala ott Leu got Ala acc Thr goa Ala 900 Ala 255 oto Leu atg Met aat Asn atc Ile g9 Ala 335 gaa Glu tto Phe got Ala 240 atc Ile aao Asn a ag Lys 990 Gly acc Thr 320 tto Phe otc Leu 672 720 768 816 864 912 960 1008 1056 1105 1118 oag atc ttc aag aag Gin Ile Phe Lys Lys taaaccccct 355 360 365 cogatctott tgg (210> 546 <211> 365 <212> PRT <213> Corynebacterium glutamioum (400> 546 Thr Val Val Pro Val Tyr Leu Ala Glu Leu Ala Pro Leu Glu Ile Arg 1 5 10 Gly Ser Leu Thr Gly Arg Asn Glu Leu Ala Ile Val Thr Gly Gin Leu 25 Leu Ala Phe Val Ile Asn Ala Leu Ile Ala Val Thr Leu His Gly Val 40 WO 01/00805PTIi0/96 PCT/IBOO/00926 Ile Val1 Le u Val1 Val1 Gly Gly 145 Ile Tyr Ala Leu Ile 225 Ala Met Val Gi y Val1 305 Phe Val Asp Asp Ala Val1 Arg His Gin 130 Glu Giy Tyr Va i Ile 210 Ile Gi y Ile Ala Ile 290 Leu Ser Thr His Gi y Leu As n Thr Ser 115 Ala ValI Val Gly Val 195 Aia Gly Thr Leu Val 275 Giy Ala Phe Lys Ala 355 Ile Phe Gin Pro 100 G lu Ser Leu Ala Thr 180 Ala Leu Leu Leu Val1 260 Trp Thr Leu Leu Phe 340 Aila Trp Leu Gly Giu As n Gly Ser Val1 165 Arg As n Arg Ser Leu 245 Val1 Val1 Gly Phe Ile 325 Val1 Ph e Arg Gly 70 Arg Arg Asn Gin As n 150 Ala Val1 Ile Asn Leu 230 Pro Gi y T rp Ile Phe 310 Phe Pro Th r Ile 55 Met T yr Ala Ala Val 135 Lys Gin Leu Ala Met 215 Thr Glu Phe Leu Ser 295 Pro Ala Giu Giy Met Le u Asp Lys Aia 120 Ser Trp Gin Glu Ph e 200 Asp Th r Giy Val1 Ala 280 Val1 Al a Val Thr Gin 360 Phe Arg Asp Aia 105 Leu Ser Leu Leu Giu 185 Gly Arg Thr As n Le u 265 Glu Phe Le u Val Arg 345 Ile Ala Val Met Pro 75 Ala Arg 90 Giu Met Pro Gly Lys His Vai Arg 155 Thr Gly 170 Ser Giy Ala Val Leu Asp Phe His 235 Ser Ile 250 Ser Met Ile Phe Cys Gly Vai Ser 315 Gly Val 330 Giy Arg Phe Lys Cys Giu Arg Asp Val Th r 140 Leu Ile Met Ala Arg 220 Leu Arg Gin Pro T rp 300 Giy Ile Ser Lys Al a Ser Val1 Giu Lys 125 His Leu Asn Ser Val 205 Arg Leu Pro Thr Val1 285 Gly Val1 Ala Leu Ala 365 Le u Pro Met Ile 110 Gin Met Ile Al a Ala 190 Ile Th r Ile Ph e Ph e 270 Arg Ile Gly Leu Giu 350 Pro Arg Giu Ile Ser Ser Ala Ile 175 Giu Gly Th r Al a Al a 255 Le u Met As n Ile Al a 335 Glu Ala Trp Th r Ala Se r Ile Gly 160 Met Met Gly Ph e Al a 240 Ile As n Lys Gly Thr 320 Phe Leu WO 01/00805 <210> 547 <211> 2013 <212> DNA <213> Corynebacterium glutainicum <220> <221> COS <222> (1990) <223> RXN02395 <400> 547 aaacaggaat atttaactcg acttcttaaa aaagctctat PCTIIBOO/00926 acgtaaatat cttgcccatc cagcaccacc tcattgtggt gcacaatgga gaggacacac atg tcc acc aac tct Met Ser Thr Asn Ser 9gc Gi y cct Pro caa Gin gca Ala gtt Val1 ggt Gly tac Tyr gac Asp gcc Ala tgg Trp 150 gag Glu aat As n cac His cg c Arg tta Leu tct S er t gg Trp atc Ilie g aa Giu Gly 135 cat His tcc Ser aac As n gac Asp aac Asn ati Ile t ca S er ctc Leu gct Ala cca Pro 120 atc Ile tat Tyr ttg Leu cc t Pro cac His ttc Phe gcg Ala t cg S er aac As n t cg Ser ttc Phe gtc Val1 agt Ser caa Gin 170 ga a Glu cca Pro gg a Gly ttc Phe tcc Ser 75 ttt Phe cqc Arg tct Ser ggt Gly ccc Pro 155 gca Ala tcc Ser ggc Gly ctc Leu atc Ile 60 a cg Thr gtc Val1 tac Tyr cgc Arg atc Ile 140 cca Pro ctt Leu gag Gi u gt t Val aaa Lys tgq Trp ttc Ph e ctg Leu cgc Arg 110 tcc Ser ttc Phe cac His ca g Gin 1 gag Gi u att Ile ttc Ph e tcc Ser gct Ala att Ile c tg Leu gcg Ala cct Pro 145 ga a Glu g aa Giu t cg S er ggc Gly agc Ser at g Met ggc Gly ggc Gly atg Met 130 tcc Se r gga Gly cct Pro gtc ValI gtc Val1 cct Pro a cg Th r acc Thr acg Thr 115 atg Met gaa Glu tct Ser cat His ga t Asp acc Thr ga t Asp aat As n a tg Met 100 gac Asp ttc Phe C cg Pro aca Thr tat Tyr gct Ala gca Al a t cg Ser act Thr ctc Leu ga a Glu ggc Gly ctg Leu cct Pro 165 115 163 211 259 307 355 403 451 499 547 595 643 ttc cac tgq ggc tta Phe His Trp Gly Leu 180 WO 01/00805 WO 0100805PCTJIBOOIOO926 t cc Se r ag t S er ct Leu atg Met 230 t ca Ser gaa Giu Gly ctt Leu tc Ph e 310 gig Val tcg Ser tac Tyr gcg Al a aig Met 390 gga Gly gcc Ala tac Tyr tic Ph e 215 gcg Ala gca Ala aic Ilie tic Ph e icc Ser 295 aic Ile tig Leu tgg Trp tgg Trp cg t Arg 375 gci Ala act Th r tgg ggg Trp Gly .185 cga cgc Arg Arg 200 ggt gag Giy Glu atc atc Ile Ile ati cag Ile Gin acc aac Thr Asn 265 aic aic Ile Ilie 280 aac cic Asn Leu acc ggc Thr Gly gaa tat Giu Tyr ggt gag Giy Giu 345 gca tgg Aia Trp 360 ati ict Ile Ser atc ccc Ile Pro gcc aic Ala Ile cig tat gcc ttg gtc ggc ggc gcg ttg gcg tac Leu Tyr Ala Leu Val Gly Giy 190 Ala Leu Ala Tyr Ser 195 ggc Gly aaa Lys gcc Ala gt Val1 250 aac As n tcc Se r aat Asn Ccc Pro ggc Gi y 330 gaa Giu tgg Trp cg c Arg t cc Ser a cg Thr 410 cgc Arg acc Thr a cg Thr 235 ggt Gly atc I le ica S er at Ilie acc Thr 315 agt Ser a cc Thr aic Ile ggc Gly ttc Ph e 395 aig Met gia acc Val Thr 205 gaa ggt Giu Gly 220 ctg ttt Leu Phe cag ggc Gin Gly tig atc Leu Ile gia ict Val Ser 285 tcc ttg Ser Leu 300 tig tic Leu Phe gag tic Giu Phe ati gaa Ile Giu gca igg Ala Trp 365 cgc acc Arg Thr 380 ati tig Ile Leu aac cgc Asn Arg tig Leu atc Ile gga G ly gig Val1 gcc Ala 270 ggc Gly a cg Thr cig Leu ttg Leu tc Phe 350 act Th r tig Leu aic Ile gag Glu aic Ile gcg Ala acg Thr cag Gin 255 aic Ile gig ValI cii Leu cic Le u t ca Ser 335 caa Gin cca Pro cgi Arg ci g L eu aac As n 415 agc icc Ser Ser ggt cgc Gly Arg 225 gct gca Ala Ala 240 ati at Ile Ile atc gcg Ile Ala icc aag Ser Lys gga tig Gly Leu 305 aac tig Asn Leu 320 aig gci Met Ala gci ggt Ala Gly tic gig Phe Val gaa tic Glu Phe 385 gcg tic Ala Phe 400 acc Th r 210 cic Le u acc Th r ict Ser aic Ile Gly 290 gi c Val aic Ile Gly igg T rp ggt Gly 370 g cg Ala acc Thr tic Phe aic Ile ttg Leu ggc Gly i ig Leu 275 at Ile cig Leu cca Pro aag L ys acg Th r 355 aig Met cic Leu at Ile cgg Arg ga c Asp ggc Gly gct Ala 260 acc Th r cgt Arg iii Phe i cg Ser ici Ser 340 g cg Ala tic Phe aic Ile tic Ph e ica Ser a ig Met ct Leu 245 t cg Ser ati Ile tat T yr gig Val1 agt Ser 325 tig Leu tc Phe aic Ile acc Th r ggt Gly 405 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 gia gat ggt Val Asp Gly iii gac ggc Phe Asp Gly 420 WO 01/00805 WO 0100805PCTIiBOO/00926 agt tca tcc aag Ser Ser Ser Lys 425 ctg tac tcg atc Leu Tyr Ser Ile 440 ttt gtt acc tct Phe Val Thr Ser 455 tcc caa ggt aac Ser Gin Giy Asn 470 ctg tgc atg atg Leu Cys Met Met tcc gcg ctg act Ser Ala Leu Thr 505 ttt gcg ctg gtg Phe Aia Leu Vai 520 tcc aca gac cca Ser Thr Asp Pro 535 tct aac gcg gtg Ser Asn Ala Val 550 ctc tcc atc gaa Leu Ser Ilie Giu gat tcc acc gct Asp Ser Thr Ala 585 gaa ggc aat gat Giu Gly Asn Asp 600 ggt tgq aca ccg Gly Trp Thr Pro 615 gaa cag gtg ctg ttt G iu aca Thr gcc Ala cct Pro qgc Giy 490 gg t Gly ttg Leu g cc Ala gtt Val1 cct Pro 570 ga t Asp gt t Val1 gaa Giu Gin ccg Pro gat Asp qca Al a 475 atc Ile ctg Leu atc Ile gct Al a cg t Arg 555 gca Ala cac His ga t Asp t cg Val ttc Ph e t cc Ser 460 cca Pro gcg Ala ca q Gin gtq Val1 att Ilie 540 ggc Giy gag Glu atc Ile tat Tyr acc Ph e 430 ttg Leu tcc Ser aaa Lys gt c Val1 ctc Leu 510 gct Ala caa Gin gaa Glu ggt Gly ga c Asp 590 ttc Phe gat Asp atc Ile gt g Vali tta Le u atg Met 495 acc Thr att Ile cg c Arg ga a Giu Cgt Arg 575 tgg Trp acc Th r atg Met ttt Phe gtg Vali atc Ile 480 ctg Le u att Ile gcc Al a tat Tyr cac His 560 gga Gly tat Tyr acc Th r ttc Ph e gtg Val1 atg Met 465 gtg Val1 ctt Leu ttg Le u ttt Phe gca Al a 545 gg C Giy g cg Ala c ag Gin ggc Gly agc S er ct g Leu 450 gga Gly gtg Val1 act Thr atc Ile att Ile 530 a ag Lys gac Asp ggt Gly cg C Arg aaa Lys 610 aac As n 435 gca Al a acg Thr ttc Phe gg t Gly qcc Al a 515 aag Lys g ca Al a gac Asp gCt Ala acc Thr 595 tgg T rp ctt Leu gt a Val1 atg Met tgg Trp gg C Gly 500 att Ile gac Asp gcc Ala ttc Phe acc Thr 580 qac Asp gcc Ala C cg Pro ttC Ph e agc Ser gga Gly 485 ga a Glu ccg Pro tta Le u atc Ile gag Giu 565 ttc Phe gaa Giu ga t Asp 14 11 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2013 gaa ggc gaa gtg gac gcg aaa aag Ser Thr Giu Glu Gly Giu Val Asp Ala Lys Lys gat taaaaaataa cgactggctg gga Asp 630 <210> 548 <211> 630 <212> PRT <213> Corynebacteriun glutamicum WO 01/00805 WO 0100805PCTJIBOO/00926 (400> 548 Met Ser Thr Asn Ser Gly Asn Asn Leu Pro Giu Ser Gin Giu Ser Pro 1 5 10 Glu Giu Pro His Tyr Pro His Asp Thr His Pro Gly Leu Val Pro Gly 25 Ile Ser Vai Asp Ala Gin Arg Asn Lys Phe Gly Leu Asp Lys Thr Val 40 Phe Gly Val Thr Ala Ala Leu Ile Leu Ala Phe Ile Ala Trp Gly Ile 55 Ser Ser Pro Asp Ser Val Ser Ser Vai Ser Ser Thr Met Phe Ser Trp 70 75 Ala Met Thr Asn Thr Gly Trp Leu Leu Asn Phe Val Met Leu Ile Gly 90 Ile Gly Thr Met Leu Tyr Ile Ala Phe Ser Arg Tyr Gly Arg Ile Lys 100 105 110 Leu Gly Thr Asp Giu Asp Glu Pro Glu Phe Ser Arg Phe Ser Trp Ile 115 120 125 Ala Met Met Phe Giy Ala Gly Ile Gly Val Giy Ile Phe Phe Phe Gly 130 135 140 Pro Ser Giu Pro Leu Trp His Tyr Leu Ser Pro Pro Pro His Thr Vai 145 150 155 160 Glu Gly Ser Thr Pro Giu Ser Leu His Gin Ala Leu Ala Gin Ser His 165 170 175 Phe His Trp Giy Leu Ser Ala Trp Gly Leu Tyr Ala Leu Val Gly Gly 180 185 190 Ala Leu Ala Tyr Ser Ser Tyr Arg Arg Gly Arg Val Thr Leu Ile Ser 195 200 205 Ser Thr Phe Arg Ser Leu *Phe Gly Giu Lys Thr Glu Gly Ile Ala Gly 210 215 220 Arg Leu Ile Asp Met Met Ala Ile Ile Ala Thr Leu Phe Gly Thr Ala 225 230 235 240 Ala Thr Leu Gly Leu Ser Ala Ile Gin Val Gly Gin Giy Val Gin Ile 245 250 255 Ile Ser Gly Ala Ser Glu Ile Thr Asn Asn Ile Leu Ile Ala Ile Ile 260 265 270 Ala Ile Leu Thr Ile Gly Phe Ilie Ile Ser Ser Val Ser Gly Val Ser 275 280 285 Lys Gly Ile Arg Tyr Leu Ser Asn Leu Asn Ile Ser Leu Thr Leu Giy 290 295 300 Leu Val Leu Phe Val Phe Ile Thr Gly Pro Thr Leu Phe Leu Leu Asn 305 310 315 320 WO 01/00805 PCTIBOO/00926 Leu Ilie Pro Ser Ser Val Leu Glu Tyr Gly Ser Giu Phe Leu Ser Met 325 33033 Ala Gly Lys Ser Leu Ser Trp Gly Giu Glu Thr Ilie Giu Phe Gin Ala 340 345 350 Gly Trp Thr Ala Phe Tyr Trp Ala Trp Trp Ilie Ala Trp Thr Pro Phe 355 360 365 Val Gly Met Phe Ilie Ala Arg Ile Ser Arg Gly Arg Thr Leu Arg Giu 370 375 380 Phe Ala Leu Ilie Thr Met Ala Ile Pro Ser Phe Ile Leu Ile Leu Ala 385 390 395 400 Phe Thr Ilie Phe Gly Gly Thr Ala Ilie Thr Met Asn Arg Glu Asn Val 405 410 415 Asp Gly Phe Asp Gly Ser Ser Ser Lys Giu Gin Val Leu Phe Asp Met 420 425 430 Phe Ser Asn Leu Pro Leu Tyr Ser Ile Thr Pro Phe Ile Leu Ile Phe 435 440 445 Val Leu Ala Val Phe Phe Val Thr Ser Ala Asp Ser Ala Ser Val Val 450 455 460 Met Gly Thr Met Ser Ser Gin Gly Asn Pro Ala Pro Asn Lys Leu Ile 465 470 475 480 Val Val Phe Trp Gly Leu Cys Met Met Gly Ilie Ala Val Val Met Leu 485 490 495 Leu Thr Gly Gly Glu Ser Ala Leu Thr Gly Leu Gin Asn Leu Thr Ile 500 505 510 Leu Ilie Ala Ilie Pro Phe Ala Leu Val Leu Ilie Val Met Ala Ile Ala 515 520 525 Phe Ilie Lys Asp Leu Ser Thr Asp Pro Ala Ala Ile Arg Gin Arg Tyr 530 535 540 Ala Lys Ala Ala Ilie Ser Asn Ala Val Val Arg Gly Leu Giu Giu His 545 550 555 560 Gly Asp Asp Phe Glu Leu Ser Ile Glu Pro Ala Glu Glu Gly Arg Gly 565 570 575 Ala Gly Ala Thr Phe Asp Ser Thr Ala Asp His Ile'Thr Asp Trp Tyr 580 .585 590 Gin Arg Thr Asp Giu Glu Gly Asn Asp Val Asp Tyr Asp Phe Thr Thr 595 600 605 Gly Lys Trp Ala Asp Gly Trp Thr Pro Giu Ser Thr Glu Glu Gly Giu 610 615 620 Val Asp Ala Lys Lys Asp 625 630 WO 01/00805 WO 0100805PCTIIBOO/00926 <210> 549 <211> 2007 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1984) <223> RXN02348 <400> 549 aaagacccga gccgaagccc tggcctgcgc atacttcctt gtcaacgctc gctgggatta ggtcttttct gagcgctagc atttctccac tcaaaggagc atg ctt aac cgc atg Met Leu Asn Arg Met aaa Lys ctc Leu

CCC

Pro ga a Glu acc Thr gct Ala a aa Lys ggc Gly gt t Val1 cgc Arg 150 atc Ile agt Ser act Thr ctg Leu gt C Val at c Ile ga c Asp aac As n gCC Ala ctC Leo 135 ttC Ph e caa Gin g CgC Al a ctc Leo tac Tyr act Th r act Thr aac As n cg t Arg gca 'Al a 120 ag c Ser att Ile ccg Pro C9g Arg gg t Gly t Ca Ser cag Gin ttg Leo caa Gin ggg Gly 105 ttg Leu gca Ala ctg Leo ctc Leo cca Pro gcc Al a Ctt Leo gaa Glu atc Ile gga Gi y cac His ttt Phe aca Thr

CCC

Pro g9t Gly 170 aaa Lys cta Leu cac His aat As n gtc Val 75 ca a Gin t gg Trp tat Tyr gaa Giu gta Val 155 aca Thr tca Ser ggt Gly act Thr gt g Val1 60 acc Thr 9gt Gly gg a Gly ggc Gly ggc Gly 140 t ct Ser gaa Glu 9tC Val1 gt t ValI gca Aia 45 tac Tyr gtc Val1 Gly aaa Lys ga t Asp 125 t tg Leo ctc Leo aaa Lys gct Ala gtg Val1 30 ttc Phe ggc Gly aaa L ys atc Ile ttC Phe 110 gtg Vali a cg Thr gc a Al a gtc Val1 cca Pro 15 ttC Phe agc Ser atc Ilie tac Tyr ct 9 Leo 95 gtg Val qtg ValI gtt Val gt t Val 9gC Gly 175 aaa Lys 9gC Gly atg Met atc I le gt c Val1 80 gc g Al a gca Ala atc Ile atc Ile ct 9 Leo 160 aaa Lys 1 tcc Ser ga c Asp cag Gin tc Se r atg Met ctc Leu gta Val acc Thr t C S er 145 atc Ile gga Gly atc Ile cac His atg Met ctg Leo gtt Val1 gcc Ala ccg Pro 130 cca Pro gct Ala caa Gin Gi y aac As n gtg Val gtC Val gct Ala ggc Gly 115 gcg Ala agc Ser att Ile gCt tta Ala Leo acc agC Thr Ser aaa qtC Lys Val ttg tgg Leo Trp acc cga Thr Arg ttg Ctg Leo Leo 100 atg ttg Met Leo atc tct Ile Ser ttt gag Phe Gio ttt gca Phe Ala 165 115 163 211 259 307 355 403 451 499 547 595 643 gCC ttC ggC CCC atC Ala Phe Gly Pro Ile 180 atg Met ate Ile cg c Arg gt t Val1 230 cat His cct Pro ca g Gin ct g Leu t cg Ser 310 aat As n g aq Giu t cc Ser agqc S er C tg Leu 390 WO 01/00805 ttg ctg tgg Leu Leu Trp 185 ggg cac cca Giy His Pro 200 ttg att gtg Leu Ilie Vai 215 gtc ctg aca Vai Leu Thr ttt ggg gcg Phe Gly Ala gct tta ate Ala Leu Ile 265 cct gaa gcg Pro Giu Aia 280 egg att ccq Arg Ile Pro 295 cag gcc gtg Gin Aia Val ttg aaa ctg Leu Lys Leu gaa qgc cag Glu Gly Gin 345 gtg atg gtt Val Met Vai 360 gcg tac gga Ala Tyr Gly 375 tat ctg atc Tyr Leu Ile ttt Phe ga a Glu get Al a gta Val agg Ar g 250 ttg Leu gt 9 Val1 ttg Le u att Ilie ctg Le u 330 ate Ile gtg Val ctt Leu tat Tyr gt C Val1 ate Ile gag Glu a cg Thr 235 cca Pro acg Thr cg C Arg gtt Val tct Ser 315 cca Pro tat Tyr gtg Val1 gca Al a gt t Val1 395 ace Th r ttg Leu cct Pro 220 99 t Gly ate Ilie tat Tyr aac As n att Ile 300 gg t Gly cg c Arg atq Met ctg Leu gtg Val1 380 ca C His ctt Leu cag Gin 205 ttc Phe gcg Ala aga Arg ttg Leu

CCC

Pro 285 t tg Leu g cg Al a atg Met cca Pro gta Val 365 ace Th r ace Thr gca Ala 190 a c Ser caa Gin gaa Glu gtg Val1 qgg Gly 270 atg Met g cg Al a tat T yr gtg Val1 ctg Leu 350 ttc Phe gga Gly aca Thr qga ttg Gly Leu ttg tct Leu Ser gca ttt Aia Phe gcg ctc Ala Leu 240 gcg tgg Ala Trp 255 cag ggc Gin Gly ttt tat Phe Tyr ace atc Thr Ilie tca ttg Ser Leu 320 ate cgg Ile Arg 335 gtt aat Val Asn cga tcC Arg Ser ace ttg Thr Leu tgg tgg Trp Trp 400 ctt cta Leu Leu 415 gga Gly cca Pro gtg Val1 225 tac Tyr ttt Ph e gcc Ala ctc Leu gct Ala 305 ace Thr cat His gga Giy tct S er gt g Val1 385 aaa Lys ttc Ph e PCTJIBOO/00926 att Ile cat His 210 ctg Leu gct Ala tgc Cys tig Leu gcg Aia 290 a cg Thr aag Lys ace Thr ttg Leu gaa Giu 370 ctg Leu a ca Thr gcc Ala ccg Pro 195 tgg Trp ctt Leu ga t Asp gtc Val gtg Val1 275 ccg Pro gtg Val cag Gin teCe Se r ctg Leu 355 agc Se r gtc Val gcg Ala tcq Se r caa Gin gcc Al a gg t Gly atg Met gtc Val1 260 ate Ile gaa Glu ate Ile gcc Al a cg C Arg 340 ttt Ph e ct C Le u aqc Ser ctg Leu aa C As n 420 ate Ile ctg Leu gcc Al a ggC Gi y 245 atg Met aac As n ggt Gly gca Ala gtq Val1 325 aaa Lys gt a Val gcc Aia gtC Val1 ttC Ph e 405 ace Thr 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 att gtg ctc atc ggt Ile Val Leu Ilie Gly 410 att cca gaa gta Ile Pro Giu Val a cg Th r ctc Leu cag Gin ctc Leu 470 gt a Val1 tgc Cys atc Ile atg Met cac His 550 att Ile ctt Leu tat Tyr t tg Leu WO 01/00805 aaa att cac Lys Ilie His 425 atc gtg gtg Ilie Vai Val 440 gaa cgc gca Giu Arg Ala 455 gat cag cca Asp Gin Pro ttt cca cat Phe Pro His gtg aaa gac Val Lys Asp 505 gtc caa gaa Val Gin Glu 520 gaa gtg ctc Giu Val Leu 535 ctt ggt tat Leu Gly Tyr gac cca aca Asp Pro Thr cgg agc agg Arg Ser Arg 585 ctt tcg atg Leo Ser Met 600 caa cca agc gac Asp a tg Met gag Glu cac His ggc Gly 490 ctc Leu cac His cat His ttt Phe tgg Trp 570 ttg Leo gaa Glu aaa gg t Gly cgg Arg ctg Leu aat Asn 475 acc Thr aag Lys gtt ValI cac His gat Asp 555 ga t Asp cct Pro cgt Arg acc gg C Gly acc Thr ga a Glu 460 att Ilie agc Ser ctt Leu ccg Pro gcc Ala 540 gag Glu aac As n gga Gly aat As n atc tgg Trp tgg Trp 445 ctt Leu gg t Giy ga t Asp tta Leo cac His 525 c cg Pro cag Gin gcc Ala aag Lys cag Gin 605 ctt Leu 430 gag Glu ccc Pro ctg Leo act Thr tac Tyr 510 gtq Vali atc Ile aac As n acc Th r att Ile 590 gca Aila cca Pro tgg Trp a tg Met cgt Arg gtc ValI 495 cga Arg cca Pro aga Arg ct g Leo tac Tyr 575 gct Ala tct Ser cta Leu gga Gly ga t Asp aaa Lys 480 c cg Pro gag Giu cca Pro gtc Val cct Pro 560 ttc Phe ggc Giy cga Arg ctt Leo agt Ser aag Lys 465 gtt Vali t tg Leo atc Ile gag Giu gtg Val 545 gag Gi u ctg Leo t gg Trp act Thr att Ile ga c Asp 450 ttc P he gcc Ala t ca Ser gtg Vai gaa Glu 530 cga Arg cat His tct Ser cg t Arg gag Giu 610 g cg Ala 435 cgc Arg t tg Leo gaa Glu ttg Leo atc Ile 515 cg c Arg gt t Vali ctc Leo gcc Ala ga t Asp 595 tct S er gcc Al a gtc Vali gag Glu gtg Val1 gtt Vali 500 gtt Val g cg Aia gat Asp cat His ctg Leo 580 cg t Arg ttc Phe PCTIBOO/00926 gtg 1411 Vali aat 1459 As n aaa 1507 Lys gca 1555 Ala 485 cgc 1603 Arg cga 1651 Arg gaa 1699 Glu ctg 1747 Leo gcc 1795 Ala 565 act 1843 Thr ttg 1891 Leo aaa 1939 Lys 1984 acq gtt gga aca gag ctg cac ctt Gin Pro Ser Lys Thr Ilie Thr Val Giy Thr Glu Leo His Leo 615 taatcaggca gttgctggcc aac <210> 550 <211> 628 <212> PRT <213> Corynebacteriom giotamicom <400> 550 2007 Met Ser Asp Gin Ser Met Leu ValI Thr Ser 145 Ile Al a G ly Pro ValI 225 Tyr Phe Ala Leu Ala 305 Thr WO 01/00805 Leu Asfl Arg Gly Gin Ala Ile Gly Thr His Asn Lys Met Val Leu Leu Val Thr Val Ala Leu 100 Ala Gly Met 115 Pro Ala Ile 130 Pro Ser Phe Ala Ile Phe Phe Giy Pro 180 Ile Pro Gin 195 His Trp Ala 210 Leu Leu Gly Ala Asp Met Cys Val Val 260 Leu Val Ile 275 Ala Pro Giu 290 Thr Val Ile Lys Gin Ala Arg Gly Ser Gin Leu Gin Gly 105 Le u Al a Leu Leu Trp 185 Pro Val1 Th r Al a Ile 265 Ala Pro Val Leu Pro Ala Leu Glu Ile Gly His Ph e Th r Pro Gly 170 Phe Giu Ala Val1 Arg 250 Leu Val1 Leu Ile Leu Lys Leu His As n Val1 75 Gin Tro Tyr Giu ValI 155 Th r Val1 Ile Giu Thr 235 Pro Thr Arg Val1 Ser 315 Pro Ser Gly Thr Val1 Th r Gly Gi y Gly Gly 140 Ser Glu Thr Leu Pro 220 Gly Ile Tyr Asn Ile 300 Gly Arg Pro Phe Ser Ile Tyr Leu Val1 Val1 Val Val Gly 175 Gly Leu Ala Ala Ala 255 Gin Phe Thr Ser Ile PCTIBOO/00926 Lys Gi y Met Ile Val Al a Al a Ile Ile Le u 160 Lys Le u Se r Phe Leu 240 T rp Gly Tyr Ile Leu 320 Arq WO 01/00805 PCT/IB00/00926 325 330 335 His Thr Ser Arg Lys Glu Glu Gly Gin Ile Tyr Met Pro Leu Val Asn 340 345 350 Gly Leu Leu Phe Val Ser Val Met Val Val Val Leu Val Phe Arg Ser 355 360 365 Ser Glu Ser Leu Ala Ser Ala Tyr Gly Leu Ala Val Thr Gly Thr Leu 370 375 380 Val Leu Val Ser Val Leu Tyr Leu Ile Tyr Val His Thr Thr Trp Trp 385 390 395 400 Lys Thr Ala Leu Phe Ile Val Leu Ile Gly Ile Pro Glu Val Leu Leu 405 410 415 Phe Ala Ser Asn Thr Thr Lys Ile His Asp Gly Gly Trp Leu Pro Leu 420 425 430 Leu Ile Ala Ala Val Leu Ile Val Val Met Arg Thr Trp Glu Trp Gly 435 440 445 Ser Asp Arg Val Asn Gin Glu Arg Ala Glu Leu Glu Leu Pro Met Asp 450 455 460 Lys Phe Leu Glu Lys Leu Asp Gin Pro His Asn Ile Gly Leu Arg Lys 465 470 475 480 Val Ala Glu Val Ala Val Phe Pro His Gly Thr Ser Asp Thr Val Pro 485 490 495 Leu Ser Leu Val Arg Cys Val Lys Asp Leu Lys Leu Leu Tyr Arg Glu 500 505 510 Ile Val Ile Val Arg Ile Val Gin Glu His Val Pro His Val Pro Pro 515 520 525 Glu Glu Arg Ala Glu Met Glu Val Leu His His Ala Pro Ile Arg Val 530 535 540 Val Arg Val Asp Leu His Leu Gly Tyr Phe Asp Glu Gin Asn Leu Pro 545 550 555 560 Glu His Leu His Ala Ile Asp Pro Thr Trp Asp Asn Ala Thr Tyr Phe 565 570 575 Leu Ser Ala Leu Thr Leu Arg Ser Arg Leu Pro Gly Lys Ile Ala Gly 580 585 590 Trp Arg Asp Arg Leu Tyr Leu Ser Met Glu Arg Asn Gin Ala Ser Arg 595 600 605 Thr Glu Ser Phe Lys Leu Gin Pro Ser Lys Thr Ile Thr Val Gly Thr 610 615 620 Glu Leu His Leu 625 <210> 551 WO 01/00805 <211> 1035 <212> DNA <21.3> Corynebacterium glutarnicum <220> <221> CDS <222> (1012) <223> RXN00297 <400> 551 aacaagqcac cggaaaacaa gcaacgcacc ttgcagaggt qgt aaaacaacat ggcacaataa cgatcatgca acaggtgctc atg Met PCT/IBOO/00926 :ttaagc atcttggagc ggt ttc acg gtg Gly Phe Thr Val qtg Val1 t tg Leu gcc Ala a cg Thr gta Val gcc Ala ggt Gly gtg Val1 acc Thr gtg Val1 150 g cg Ala gtq gtc Val1 cat His gcg Ala tct Se r ctg Leu t cg Ser gq t Gly 105 ccc Pro atc Ile ctc Leu gg t Gly cag atc Ile gcc Ala ttg Leu ctg Leu ttt Phe gtg Val atc Ile gtg Val1 atg Met ctg Leu att Ile 170 cca gga Gly cag Gin ttg Leu aac As n ttc Ph e 75 atc Ile cct Pro att Ile gag Glu gtc Val1 155 gct Ala gtg ggt Gly cct Pro ga t Asp gtg Val ctc Le u atg Met gca Al a ttc Ph e 125 ctg Leu cca Pro t ct Ser atg tgg Trp ttg Leu 30 cgg Arg gtc Val1 atg Met cta Leu g cc Ala 110 cag Gin acc Thr cta Leu t tg Leu t tg att Ile 15 agc Ser gtc Val1 att Ilie Arq gct Ala 95 tat T yr gtg ValI aac As n acc Th r act Th r 175 g cg ctc Leu ctg Leu acc Th r gca Al a ttc Phe 80 gcg Ala att Ile gcg Al a aag Lys aac As n 160 tcg Ser aat 1 gt Gi y ttt Phe aag Lys ctc Leu gtg Val tcc Ser ttg Le u ttc Phe aaa Lys 145 acc Thr a tg Met gct a ga Arg gtc Val tca Ser tct Ser atc Ile tac Tyr gat Asp tac Tyr 130 tcc Ser atg Met agc Ser tca aga Ar g tat Tyr ga t Asp gc g Ala aaa Lys gcc Ala ga t Asp 115 gca Ala acc Th r gtg Val1 gtg Val1 gtg ga c Asp tac Tyr acc Thr ttg Leu a ga Arq aac Asn 100 ttc Phe ccg Pro aac Asn ctc Leu ccc Pro 1.80 cca acc Th r qtg ValI t cq Ser atc Ile act Th r gcc Ala acc Thr atc Ile ttg Le u gca Ala 165 gtg Val1 ctg WO 01/00805 PCTIBOO/00926 Val Ile Ala Gin Pro Val Giu Met Leu Ala Asn Ala Ser Val Pro Leu 185 190 195 gct ttg gtg gtg ttc gga ctg tcc ttg tcc aag agc aag atc ctqgqaa 739 Ala Leu Val Val Phe Gly Leu Ser Leu Ser Lys Ser Lys Ile Leu Glu 200 205 210 aag ggg cag gta tcc agg cqc gat gtg tit acg gcg gca ctg ttc aag 787 Lys Gly Gln Val Ser Arg Arg Asp Val Phe Thr Ala Ala Leu Phe Lys 215 220 225 aat gtt ctg cac cca att gtt gcg gga ctt tta gcc tta gcc ttt ggt 835 Asn Val Leu His Pro Ilie Val Aia Giy Leu Leu Ala Leu Ala Phe Gly 230 235 240 245 atg gaa ggt act gcc ttg ttg tcg gcg gtg att ctc ggt qca ctg cca 883 Met Giu Giy Thr Ala Leu Leu Ser Ala Vai Ile Leu Gly Ala Leu Pro 250 255 260 aca gcq cag aat gtc tac acc tac gcg ttg cga ttt aga aca gct gaa 931 Thr Aia Gin Asn Val Tyr Thr Tyr Ala Leu Arg Phe Arg Thr Ala Glu 265 270 275 tcg atg gcg aga gac aca ggg gtg gtc acc aca ctc att tcc ttc ccc 979 Ser Met Ala Arg Asp Thr Giy Vai Val Thr Thr Leu Ile Ser Phe Pro 280 285 290 gta ttg gtg gcg gtc tcc att atc ttt gga tct tagggttagc attagtggtc 1032 Val Leu Val Ala Val Ser Ile Ile Phe Gly Ser 295 300 a tq 1035 <210> 552 <211> 304 <212> PRT <213> Corynebacterium glutamicum <400> 552 Met Gly Phe Thr Val Val Phe Ilie Val Ile Gly Ile Gly Trp Ile Leu 1 5 10 Gly Arg Arg Asp Thr Leu Gly Thr His Ala Gin Lys Pro Leu Ser Leu 25 Phe Val Tyr Tyr Val Ala Thr Pro Ala Leu Leu Phe Asp Arq Val Thr 40 Lys Ser Asp Thr Ser Thr Ile Phe Ser Leu Asn Phe Val Val Ile Ala 55 Leu Ser Ala Leu Ile Val Gly Phe Leu Phe Phe Leu Leu Met Arg Phe 70 75 Val Ilie Lys Arg Thr Ala Ala Val Ser Val Ile Gly Met Leu Ala Ala 90 Ser Tyr Ala Asn Ala Gly Asn Leu Gly Ile Pro Leu Ala Ala Tyr Ile 100 105 110 WO 01/00805 PCTIIBOO/00926 Leu Asp Asp Phe Thr Val Val Ile Pro Val Ilie Leu Phe Gin Val Ala 115 120 125 Phe Tyr Ala Pro Ile Thr Met Thr Ile Met Giu Met Leu Thr Asn Lys 130 135 140 Lys Ser Thr Asn Leu Val Arg Asn Leu Leu Val Thr Pro Leu Thr Asn 145 150 155 160 Thr Met Val Leu Ala Ala Ilie Ala Giy Ilie Ala Val Ser Leu Thr Ser 165 170 175 Met Ser Vai Pro Val Val Ile Ala Gin Pro Val Glu Met Leo Ala Asn 180 185 190 Ala Ser Val Pro Leu Ala Leu Val Val Phe Gly Leo Ser Leu Ser Lys 195 200 205 Ser Lys Ile Leu Giu Lys Gly Gin Val Ser Arg Arg Asp Vai Phe Thr 210 215 220 Ala Ala Leu Phe Lys Asn Val Leo His Pro Ile Val Ala Giy Leo Leu 225 230 235 240 Ala Leu Ala Phe Gly Met Glu Gly Thr Ala Leo Leu Ser Ala Val Ile 245 250 255 Leo Gly Ala Leo Pro Thr Ala Gln Asn Val Tyr Thr Tyr Ala Leu Arg 260 265 270 Phe Arg Thr Ala Giu Ser Met Ala Arg Asp Thr Gly Val Val Thr Thr 275 280 285 Leo Ile Ser Phe Pro Val Leo Val Ala Val Ser Ile Ile Phe Gly Ser 290 295 300 <210> 553 <211> 353 <212> DNA <213> Corynebacterirn glotamicom <220> <221> CDS <222> (101)..(343) <223> RXN03103 <400> 553 atcttcagag tcacttcttt cagtgtcatt tttctcggcc ctaatccccc gctggagttc aatcagcgat tgcaaccttt tagatatata aggagacaac atg tct gca aag cgt 115 Met Ser Ala Lys Arg 1 act ttt acc cgt atc ggt gcg att ctt gga gca act gca ctt gcc gga 163 Thr Phe Thr Arg Ile Gly Ala Ile Leo Gly Ala Thr Ala Leo Ala Gly 15 WO 01/00805 gtt acc ctc acc gcc tgt ggt qat.tca agc Val Thr Leu Thr Ala Cys Gly Asp Ser Ser 30 gca gcc att gaa aat qgt tct gtc aat gtc Ala Ala Ilie Giu Asn Gly Ser Val Asn Val 45 cct ggt ctt ggc ctc cgc aac cca gac aac Pro Gly Leu Gly Leu Arg Asn Pro Asp Asn 60 gtg gat gtt gct gaa tac gta att caa ctc Val Asp Vai Ala Giu Tyr Val Ile Gin Leu <210> 554 <211> 81 <212> PRT <213> Corynebacterium giutaniicum <400> 554 Met Ser Aia Lys Arg Thr Phe Thr Arg Ilie 1 5 10 Thr Ala Leu Ala Gly Vai Thr Leu Thr Ala 25 Gly Asp Giy Phe Leu Ala Ala Ile Giu Asn 40 Thr Lys Tyr Asp Gin Pro Gly Leu Giy Leu 55 Met Ser Gly Leu Asp Val Asp Vai Ala Giu 70 Arg PCTIBOO/00926 ggc gac gga ttc ctc 211 Giy Asp Giy Phe Leu acc aaa tac gat cag 259 Thr Lys Tyr Asp Gin atg agc ggt ctc gac 307 Met Ser Giy Leu Asp cgc tgatgacaaq 353 Arg Gly Ala Ile Leu Gly Cys Gly Asp Ser Ser Giy Ser Vai Asn Val Arg Asn Pro Asp Asn Tyr Val Ilie Gin Leu 75 Ala Giy Giy Se r His <210> 555 <211> 695 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> <223> RXN02993 <400> 555 gtt gct gaa tac gta gtc aac tcc atc gct gat gac aag ggc tgg gat Val Ala Giu Tyr Val Vai Asn Ser Ilie Ala Asp Asp Lys Giy Trp Asp 1 5 10 cac ccc acc atc gaa tgg cgt gaa tcc cct tct gcg cag cgt gaa acc His Pro Thr Ilie Giu Trp Arg Giu Ser Pro Ser Ala Gin Arg Giu Thr 25 WO 01/00805 WO 0100805PCT/IBOO/00926 ctc Leu aac Asn acc Thr ctc Leu act Thr gaa Giu gt t Val cag Gin 145 cca Pro gaa Glu ggc Gly gt t Val1 att Ilie gct Ala cac His gag Giu cca Pro tac Tyr ga c Asp 130 tac Tyr ttc Phe 9ggC Gly acc Thr gtt Val caa Gin gci Gly ca g Gin ga c Asp gct Ala gac Asp gc c Ala gaa Giu acc Th r a cc Thr ttc Phe 195 gaa Giu aac ggt Asn Gly cgt tca Arg Ser gct ctg Ala Leu ttg gat Leu Asp cag aag Gin Lys 100 acc tac Thr Tyr ctg acc Leu Thr ggc gac Gly Asp gac gag Asp Giu 165 gac gct Asp Ala 180 cag cga Gin Arg gaa ggc Giu Gly gag gta gac atg atc q ca Giu Vai Asp Met Ile Ala gca acc tac tcc atc Ala Thr Tyr Ser Ile gag Giu ct t Leu 70 aa c Asn gt c Val1 tct Se r act Th r ttc Ph e 150 tac T yr atc Ile ctg Le u acc Thr tcc gtc Ser Val 55 gtt cgc Val Arg ggt ttg Gly Leu aag gat Lys Asp tcc tgt Ser Cys 120 gac gcc Asp Ala 135 cgc gtt Arg Val tac ggc Tyr Gly aac gcc Asn Ala ctc acc Leu Thr 200 cca ggt Pro Gly 215 aac Asn caa Gin atc Ile gtc Val 105 gtt ValI acc Th r gtg Val1 att Ile gca Ala 185 gag Glu ttc Phe gat Asp ct g Leu 90 ctc Le u gag Giu atc Ile gaa Giu Gly 170 ctt Le u aac Asn ggc Gly ga t Asp t cc Ser ggc Giy ctg Leu ttc Ph e 140 gaa Giu aag Lys cgc Arg ggt Gly cca Pro cg c Ar g gtt Val1 gt t Val1 tcc Se r 125 ggc Gly aag Lys aag Lys atg Met gaa Giu 205 tac Tyr att Ile tcC Ser ca g Gin 110 cag Gin tac Tyr ga c Asp ga t Asp tac Tyr 190 ga c Asp ctg Leu ga a Giu gga Giy ctc Leu ggc Giy t cc Ser ggc Giy ga c Asp 175 gc t Ala t cc Ser ctt Leu acc Th r t cc Ser caa Gin aac Asn cag Gin gag Giu 160 cag Gin gac Asp gtg Val1 192 240 288 336 384 432 480 528 576 624 672 gac ctc tcc ttc ctc gac gca agc Asp Leu Ser Phe Leu Asp Ala Ser 220 210 tagtgtgacg gcttttaaaa gcc <210> 556 <211> 224 <212> PRT <213> Corynebacterium giutamicum <400> 556 Val Ala Giu Tyr Vai Val Asn Ser Ile Ala Asp Asp Lys Giy Trp Asp 1 5 10 His Pro Thr Ile Giu Trp Arg Giu Ser Pro Ser Ala Gin Arg Giu Thr WO 01/00805 Leu Ilie Gin Asn Asn Ala Giy Arg Thr His Gin Ala Leu Giu Asp Leu Thr Pro Aia Gin 100 Giu Tyr Asp Thr 115 Val Asp Ala Leu 130 Gin Tyr Giu Giy 145 Pro Phe Thr Asp Glu Gly Thr Asp 180 Giy Thr Phe Gin 195 Val Vai Giu Glu 210 PCTIBOOIOO926 Giy S er Le u Asp Lys Tyr Thr Asp Giu 165 Al a Arg Gly Giu Giu Leu 70 Asn Val1 Ser Thr Ph e 150 Ty r Ilie Leu Thr Asp 40 Val Arg Leu Asp Cys 120 Al a Val1 Gly Ala Th r 200 Gly 25 Met As n Gin Ile Val1 105 Val Thr Val Ile Ala 185 Giu Asp Ile Phe Asp Le u 90 Le u Glu Ile Glu Gly 170 Le u As n Leu Ala Gi y Asp 75 Cys Pro Ala Leu Met 155 Leu G iu Leu Se r Ala Gi y Asp Ser Gly Leu Phe 140 Giu Ly s Arg Gly Phe 220 Thr Pro Arg Val1 Val1 Se r 125 Gly Lys Lys Met Glu 205 Leu Tyr Tyr Ile S er Gin 110 Gin Tyr Asp Asp Tyr 190 Asp Asp Ser Leu Giu Gly Le u Giy Ser Gly Asp 175 Ala Ser Ala Ile Le u Thr Ser Gin Asn Gin Giu 160 Gin Asp Val1 Ser <210> 557 <211> 1590 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1567) <223> RXN00349 <400> 557 tgtgtacatc acaatggaat tcgqggctag agtatctqgt gaaccgtgca taaacgacct gtgattggac tctttttcct tgcaaaatgt tttccagcgg atg ttg agt ttt gcg 115 Met Leu Ser Phe Ala 1 acc ctt cgt ggc cgc att tca aca gtt gac gct gca aaa gcc gca cct 163 Thr Leu Arg Gly Arg Ilie Ser Thr Val Asp Ala Ala Lys Ala Ala Pro WO 01/00805 WO 0100805PCT[IBO0/00926 ccg Pro gcc Ala t ca Ser tct Ser atc Ile gtg Val1 gag G lu gag Glu tat Tyr 150 gtt Val1 ati Ile ggt Gly ctg Leu aic Ile 230 gg9t Gly c ca Pro gg t Gly gg t Gly gcg Ala acc Thr ttt Phe g tt Val1 gt t Val1 135 gg t Gly gc t Al a acc Th r ttg Leu cc t Pro 215 aaa Lys tcg cca Ser Pro gtg atg Val Met acg tca Thr Ser tac ggt Tyr Gly atc ttc Ilie Phe cat gtt His Val 105 gac cgt Asp Arg 120 gcc gag Ala Glu ttc cct Phe Pro gtg ctg Val Leu gcg ttc Ala Phe 185 cct act Pro Thr 200 gca tcg Ala Ser ccg agc Pro Ser cta Leu aat Asn aat Asn ttg Leu acc Th r gta Val ttg Leu aaa Ly s gt t Val ttg Leu 170 acg Thr ttc Phe att Ile cag Gin g tg Val 250 gcc ccg Ala Pro ttg gct Leu Ala agt gac Ser Asp 60 tac tac Tyr Tyr 75 aac atc Asn Ile ggc aag Gly Lys atc cgt Ilie Arg atc ctg Ile Leu 140 gcg ttg Ala Leu 155 ggt ggt Gly Gly atc att Ile Ilie ttc caa Phe Gin gct tat Ala Tyr 220 atc atc Ile Ile 235 att Ile gcg Al a 45 acc Th r acg Thr ggt GlIy ttg Leu tcc Ser 125 gac Asp ctt Le u gg a Gly gcc Ala aat As n 205 tct Ser gca Ala ga t Asp 30 aga Arg aag Lys cac His gt g Val1 ga c Asp 110 att Ile gag Glu g gc Gly t gg Trp acg Thr 190 gtt ValI ttg Leu tct Ser 15 ctc Leu att Ile gta Val1 gtg Val gag Giu 95 acc Th r cag Gin ttg Leu t gg Trp cag Gin 175 acg Th r gt t ValI gcg Ala gga Gly ga c Asp 255 act Th r ggc Gly ca a Gin ga t Asp 80 agg Arg aac As n gc t Ala gag Glu gca Ala 160 gt t Val1 tca Ser ggt Giy ttg Le u att Ile 240 gac Asp.

gat Asp gt t Val1 atc Ile aa g Lys ttc Ph e ggt Gly caa Gin 145 atg Met t cc Ser ttt Ph e ggt Gly caa Gin 225 gtt Val cat His att Ile cga Arg a cg Th r atg M et1 t cc Ser gcg Ala 130 t cc Ser a tg Met cta Le u ttg Leu ttt Phe 210 ttt Ph e gtg Val1 agcit Ser ttg Leu gca Ala ttg Leu ccg Pro aaa Lys 115 acc Thr cct Pro ggt Gly att Ile gga Gly 195 att Ile gg t Gly ctg Le u caa Gin ct t Le u gtg Val1 aat Asn gt c Val1 100 ctg Leu ccg Pro g cg Ala gg t Gly gct Ala 180 aag Lys gcc Ala ctt Leu ttg Leu gtg ValI tct Ser acc Thr acg Thr aac As n tct Se r cc t Pro tct Ser gct Ala 165 ttt Ph e aag Lys acg Thr gag Giu gca Ala 245 211 259 307 355 403 451 499 547 595 643 691 739 787 835 883 ttg aca ctc Leu Thr Leu caa tct ctg cag Gin Ser Leu Gin ggc atc acg ggc gct ccg Gly Ile Thr Gly Ala Pro 260 WO 01/00805 WO 0100805PCT11BOO/00926 gtg Val1 att Ile g tc Val1 aca Thr 310 gtg ValI g cg Ala ccc Pro ggt Gly gcc Ala 390 tac Tyr gtt Val1 gag Glu cgt Arg cag Gin 470 a ca Thr qtt Val1 atg Met 295 ttc Phe ggt Gly ctg Leu gtc Val1 ttg Leu 375 9gc Gly gcc Ala gc t Ala tgg T rp g ca Al a 455 aat As n g ca Ala gct Ala 280 ttg Leu g cc Ala tgt Cys a tg Met tct Ser 360 ctt Leu atc Ile acc Thr tta Leu att Ile 440 ttt Phe cag Gin agt Ser 265 gg C Gly cct Pro cqc Ar g tac Tyr gg t Gly 345 gcc Ala gc c Ala aca Th r ct g Le u g cc Ala 425 gcc Ala acc Thr cgc Arg gca Ala gtg Val g cc Ala att Ile g cg Ala 330 tct Se r gct Ala cgt Arg cca Pro aat Asn 410 act Thr cgc Arg.

aag Lys Cgg cga Ar g gg t Gly atg Met atc Ile 315 gag Glu gcg Al a gcg Ala cg a Arg atg Met 395 ga t Asp gc t Ala agg Ar g gcg Ala cag ttt Phe t tg Leu gag Glu 300 gct Ala t gg Trp ttt Phe att Ile ttc Ph e 380 ctt Leu caa Gin tca Ser cta Leu aat Asn 460 a ga ttc Phe ggc Gly 285 tcc Ser ggt Gi y tcc Ser tat T yr gct Al a 365 t tg Leu c ca Pro aca Thr tca Ser cgt Arg 445 gag Giu aaa ga a Glu 270 att Ile gc t Ala gg C Gly t cg Se r tac Tyr 350 gca Ala att Ile gg t Gly ctc Le u ctt Leu 430 cg t Arg ttc Ph e cgt aca Thr cag Gin gca Ala gtc Val gtg Val1 335 ctc L eu aca Th r cca Pro cta Leu atg Met 415 g cc Ala cca Pro t cc Ser cca ctc Le u ctt Leu gca Ala acc Thr 320 att Ile ttc Phe gca Ala ccg Pro gc a Ala 400 ggt Gly gct Ala cca Pro t tc Phe aag ct g Le u tct Ser cct Pro 305 gca Al a att Ile gtt ValI gtt Val t tg Leu 385 att Ile ttc Ph e ggC Gly cg c Arg cag Gin 465 ttt Phe gaa G lu 290 aat Asn qcg Ala g c9 Ala gtt Val ggt Gly 370 att Ile tac Tyr acc Thr gtg Val ttc Ph e 450 gag Glu aco Thr 275 atc Ile tat Tyr qcc Ala gggi Gi y tat.

Ty r 355 ttc Phe gtg Val1 cg C Arg aac Asn gtt Val1 435 aac Asn gaa Glu ggc Gly ttg Leu t cg Ser ttc Phe ctt Leu 340 tta Leu act Thr g cg Ala gga Gly att Ile 420 ttg Leu cca Pro gct Al a ggC Gly Cat His tct Ser gca Ala 325 act Thr ggC Gi y gg t Gly att Ile a tg Met 405 g cg Ala ggt Gly tac Tyr gag Glu 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1590 act aat cag aga ttc Arg Gin Arg Lys Arg Pro Lys Thr Asn Gin Arg Phe ggt aat aaa agg Gly Asn Lys Arg taaaaatcaa cctgcttagg cgt WO 01/00805 <210> 558 <211> 489 <212> PRT <213> Corynebacterium giutamicum <400> 558 Met Leu Ser Phe Ala Thr Leu Arg Gly PCTIBOO/00926 1 Ala Asp Asp Va 1 Ile Lys Ph e Gly Gin 145 Met Ser Ph e Giy Gin 225 Vai Ile Lys His Ilie Arg Thr Met Ser Ala 130 Ser Met Leu Leu Ph e 210 Phe Val Thr Ala Aia Ser Gin Leu Leu Ala Val Leu Asn Pro Val 100 Lys Leu 115 Thr Pro Pro Ala Gly Gly Ile Ala 180 Giy Lys 195 Ile Aia Gly Leu Leu Leu Giy Aia 260 5 Pro Val1 Ser Thr Thr Asn Ser Pro Se r Al a 165 Phe Lys Thr Glu Ala 245 Pro Pro Pro Aia Giy Ser Gly 55 Ser Ala 70 Ile Thr Val Phe Giu Val Giu Vai 135 Tyr Giy 150 Vai Aia Ile Thr Gly Leu Leu Pro 215 Ile Lys 230 Gly Leu Val Thr Ser Val1 40 Thr Tyr Ile His Asp 120 Ala Ph e Val1 Ala Pro 200 Ala Pro Th r Ala Pro 25 Met Ser Gi y Ph e Val1 105 Arg Giu Pro Leu Phe 185 Th r Ser Ser Leu Ser 265 Arg 10 Leu Asn Asn Leu Thr 90 ValI Leu Lys ValI Leu 170 Thr Phe Ile Gin Val1 250 Ala Ile Ala Leu Ser T yr 75 As n Gly Ile Ile Ala 155 Gly Ile Ph e Al a Ilie 235 Gin Ar g Ser Pro Al a Asp Tyr Ile Lys Arg Le u 140 Leu Gly Ile Gin Tyr 220 Ile Ser Ph e Thr Ile Ala Thr Th r Gly Leu Se r 125 Asp Leu Gly Ala Asn 205 Ser Ala Leu Phe Val Asp Arg Lys His ValI Asp 110 Ile Giu Gly Trp Thr 190 Val Leu Ser Gin Glu 270 Asp Leu Ilie Val Val Glu Thr Gin Le u Trp Gin 175 Thr Val1 Ala Gly Asp 255 Th r Ala Thr Gly Gin Asp Arg As n Ala Giu Ala 160 Val1 Ser Gly Leu Ile 240 Gly Leu Leu Phe Thr Gly Gly Ile Val Ala Gly Val Gly Leu 275 280 Ile Gin Leu WO 01/00805 PCTIIBOO/00926 His Val Met Leu Pro Ala Met Glu Ser Ala Ala Ala Ser Pro 305 Ala Ile Val Vai Leu 385 Ilie Phe Gly Arg Gin 465 Thr Giu 290 Asn Ala Ala Val1 Gly 370 Ile Tyr Thr Vali Phe 450 Glu Asn Ile Tyr Ala Giy T yr 355 Phe Vali Arg As n Vai 435 Asn Giu Gin 295 300 Phe Giy Leu Val1 Leu 375 Gly Al a Al a Trp Ala 455 Asn Asn Ala C ys Met Ser 360 Leu Ile Thr Leu Ile 440 Ph e Gin Lys Arg Tyr Gly 345 Ala Ala Thr Le u Ala 425 Ala Th r Ar g Arg Ala Trp Phe Ile Phe 380 Leu Gin S er Leu Asn 460 Arg Val Val1 335 Le u Thr Pro Leu Met 415 Ala Pro Ser Pro Thr 320 Ile Phe Ala Pro Al a 400 Gi y Al a Pro Ph e Lys.

480 'z210> 559 (211> 492 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (469) <223> RXN03095 <400> 559 aacgcctcaa ttagtgccag accttgccga ccgjcagacca aacttcacca tttcaaacca tccctagcca caacaacggc agttgtgcaa tgatctqcgt atg aat gca gat aag 115 Met Asn Ala Asp Lys 1 aaa atg tgc gga atg aac ccg gat agc caa tac gtc gaa ctt gcc gtc 163 Lys Met Cys Gly Met Asn Pro Asp Ser Gin Tyr Val Glu Leu Ala Val 15 gaa Glu gca Ala gtC Val1 a tg Met aaa Lys cag Gin cg a Arg WO 01/00805 gtt ttc gga Val Phe Gly ctt cga aac Leu Arg Asn gat aaa tcc Asp Lys Ser gcc cga atc Ala Arg Ile ctc: acc aat Leu Thr Asn gcg gaa cac Ala Glu His 105 gaa cga gaa Giu Arq Giu 120 gcg gac gcc act cga gtt cgc Ala Asp Ala Thr Arg Val Arg gaa ctt tcc gta aac cac cic Giu Leu Ser Val Asn His Leu 45 gca gtt tcc caa cac ctc gcc Ala Val Ser Gin His Leu Ala 60 acc cgt caa gaa ggt caa cga Thr Arg Gin Glu Gly Gin Arg 80 gca tca cag cta gtc tcc gac Ala Ser Gin Leu Val Ser Asp gcg gac ggc cag act ccc cca Ala Asp Gly Gin Thr Pro Pro 110 tgaccaccca cagtcaccaa gaa atc Ile gac Asp ctg Leu ttc Ph e att Ile 100 cac His PCTIBOO/00926 tig 211 Leu atc 259 Ile cgc 307 Arg tac 355 Tyr ttt 403 Phe cac 451 His <210> 560 <211> 123 <212> PRT <213> Corynebacterium giutamicum <400> 560 Met Asn Ala Asp Lys Lys Met Cys Gly 1 5 Val Giu Leu Ala Val Giu Val Phe Gly 25 Val Arg Ile Ile Leu Ala Leu Arg Asn His Leu Ala Asp Ile Val Asp Lys Ser 55 Leu Ala Arg Leu Arg Met Ala Arg Ile .70 Gin Arg Val Phe Tyr Lys Leu Thr Asn Ser Asp Ala Ile Phe Gin Ala Giu His 100 105 Pro Pro His His His Arg Giu Arg Glu 115 120 <210> 561 Met 10 Leu Ser Pro ValI Gi u 90 Th r Gin Asn Leu Gly Ala Ser 75 His Ile Se r Pro Ala Glu Al a Thr Ala Ala Se r Al a Ser Ser Gin Gln Gly 110 Gin Thr Val Gin Glu Leu Gin Tyr Arg Asn His Gly Val1 Th r WO 01/00805 <211> 591 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(568) <223> RXN03160 <400> 561 gagcatatgg tgcgcgcggC cgatatgctg atcaattcca PCT/1BO0100926 accccgatcc gcacgcttaa cttctgccaa aaagtcqttt tgaccataag ctaagcqatt ga a Glu

CCC

Pro cgc Ar g cta Leu ca C His tgg Trp acc Th r gtt Val1 CCg Pro ctc Leu 150 atc Ile acc Thr t gg Trp Ct t Leu CgC Arg

CCC

Pro tcc Se r ttc Phe ggg Gly 135 ggc Gly C9 C r g ca c His gat Asp gg a Gly gg t Gly t tg Leu ag c Ser 105 CgC Arg cgg Arg ttc Ph e gga Gly Cg c Arg gga Gly tcc Ser 75 ctc Leu a cg Thr Cac His cca Pro gg C Gly tat Tyr caa Gin tCg Ser 60 9gC Giy atg Met ctc Leu C9 9 Arg tcg Ser 140 gtg Ctg ggc ttC Vai Leu Giy Phe ttc CgC acg gaa Phe Arg Thr Glu 30 tat tCC gag atC Tyr Ser Giu Ile tCg cag gtc ggt Ser Gin Val Giy atg qcg atc gCC Met Ala Ile Aia 80 gcq gCg ttC gcc Ala Ala Phe Ala ggc ctg CtC gcC Gly Leu Leu Ala 110 gca tgg cgc ggt Ala Trp Arg Gly 125 ggg gtC ggc ctc Gly Val Gly Leu gtg aat cga Vai Asn Arg agc gct ttC Ser Ala Phe ttc gtg gag Phe Val Glu gta gcg Ctc Val Ala Leu atg atg ctg Met Met Leu tgg ctc atg Trp Leu Met ctg ctt ttt Leu Leu Phe gca gcg gtc Ala Ala Val 115 tca atg gCt Ser Met Ala 130 ggg gca tcg Gly Ala Ser 145 att gca Ile Ala 9gC Gly Cgg Arg agc Ser ggC Gi y ttt Phe ga t Asp 100 gCC Ala tCC Ser Cgg Arg ggC Gly Cgg Arg caa Gin tac Tyr acc Thr gcg Ala gtc ValI a cg Thr gtg Val1 115 163 211 259 307 355 403 451 499 547 CCC caa cgg ggt taacacaCct tggggttCaa tca Pro Gin Arg Gly <210> 562 <211> 156 <212> PRT <213> Corynebacterium giutamicum WO 01/00805 WO 0100805PCT/1BOO/00926 <400> 562 Val Asn Arg Ile Ala Glu Ilie Ala Arg Ser Phe 1 5 10 Ser Ala Phe Gly Gly Pro Thr Ala His Leu Gly Phe Val Glu Arg Arg Arg Trp Leu Asp Asp Arg Val Ala Leu Ser Gin Leu Leu Pro Gly Pro Gly Met Met Leu Gly Tyr His Arg Ala Gly Phe Ser 70 75 Trp Leu Met Phe Thr Trp Pro Ser Leu Ala Leu 90 Leu Leu Phe Asp Ala Thr Ser Ala Ser Trp Thr 100 105 Ala Ala Val Ala Val Val Phe Lys Arg Ser His 115 120 Ser Met Ala Ser Thr Pro Gly Arg Arg Pro Pro 130 135 Gly Ala Ser Arg Val Leu Gly Pro Pro Gln.Arg 145 150 155 <210> 563 <211> 645 <212> DNA <213> Corynebacterium glutamicum <2 <221> CDS <222> (101)..(622) <223> RXN02955 <400> 563 atgcactggc tcccatcaaa gacgaattct tgacctcaga aagcagtgcg cgccgctgat acticaggag gtgcggcatc Gly Thr Glu Val1 Ile Ph e Leu Arg Gly Phe Glu Ile Gly Ala Ala Ala Gly Leu icc atc gig tgc gtc act gcc Ser Ile Val Cys Val Thr Ala cac agc cca tca acg tgg tct His Ser Pro Ser Thr Trp Ser igg cta tct ttc gca ggt agt Trp Leu Ser Phe Ala Gly Ser cagq Gin ga a Gi u 30 ttc Ph e gig Val 15 cta Leu ctc Leu aitccagcgt gaactctacg aig atg aai itt aag 115 Met Met Asn Phe Lys 1 agc cgc cag gtg cig 163 Ser Arg Gin Val Leu aag ctg ttg ttc gig 211 Lys Leu Leu Phe Val gga gag cgt gga cat 259 Gly Glu Arg Gly His WO 01/00805 WO 0100805PCTAIBOO/00926 att Ile gtc Val1 gg C Gly cag Gin ccg Pro gaa Giu gaa Glu 150 gtt Vai cag Gin atc Ile act Thr gcg Ala 120 gcc Al a cct Pro ga t Asp g tc Vai tgg Trp qcg Aila 105 gg t Gi y aca Th r cg a Arg gcg Ala cag Gin tac Tyr ctt Le u atc Ilie ga a Giu 140 ga c Asp aaa Ly s ttg Le u gct Aia ttg Leu 110 t tg Leu cct Pro cta Le u ctg cct Leu Pro att gtt Ile Vai 80 gca tca Aia Ser gqa tgg Giy Trp ttc gcg Phe Ala tac ccc Tyr Pro gag gcc Giu Ala 160 gtt tct gcg Vai Ser Aia gtt tit gcg Val Phe Ala atc gcg tgg Ile Ala Trp gtg tat gtt Val Tyr Val 115 atc atc gat Ile Ile Asp 130 ctt gtt gat Leu Val Asp 145 caa agc gct Gin Ser Ala cag cgg Gin Arg atc ctc Ile Leu aat cag Asn Gin 100 gtc atc Vai Ile ctc atc Leu Ile gaa tca Giu Ser ata gat Ile Asp 165 307 355 403 451 499 547 595 tct gca agt tca gcg gaa ggt agg aac Ser Aia Ser Ser Ala Giu Giy Arg Asn 170 ctg <210> 564 <211> 174 <212> PRT <213> Corynebacterium glutamicum <400> 564 Met Met Asn Phe Lys Ser Ilie Vai Cys 1 5 Ser Arg Gin Val Leu His Ser Pro Ser Lys Leu Leu Phe Val Trp Leu Ser Phe Gly Giu Arg Gly His Ilie Ala Val Asp Vai Ser Ala Gin Arg Val Leu Gin Vai 70 Vai Phe Ala Ilie Leu Giy Met Ile Trp Ile Aia Trp Asn Gin Gin Leu Thr Ala taatatgttg tcqccagcag Val1 10 Thr Aila Phe Ile Gly 90 Leu Thr Trp Giy Ile Vai 75 Giy Pro Val1 Leu Leu Leu Ile Ala Giy Phe Se r Phe Pro Vali Ser Trp WO 01/00805 PCT/TBOO/00926 100 105 110 Val Tyr Val Val Ile Pro Ile Ala Gly Val Phe Ile Ala Leu Phe Ala 115 120 125 Ile Ile Asp Leu Ile Glu Val Ala Thr Gly Lys Giu Giu Pro Tyr Pro 130 135 140 Leu Val Asp Glu Ser Giu Giu Pro Arg Asp Leu Asp Giu Leu Glu Ala 145 150 155 160 Gin Ser Aia Ile Asp Ser Ala Ser Ser Ala Glu Giy Arg Asn 165 170 565 <211> 754 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(754) <223> RXN03109 (400> 565 actggcgctt cccgatggtc aatcattggc ctttggtgcc caaactqcg agjttgttgct ccgcgcatcc cgcgaactgt atgtgcaggg cggcgagtag atg gtt gtg aag gag Met Val Val Lys Giu gt t Val aag L ys gtg Val c cg Pro ca 9 Gin gtq Val1 ttg Leu gtg gaa Glu a cc Thr qcg Ala gat Asp gcg Ala c tg Leu qtg Val1 105 99C aag Lys gtg Val1 t cg Se r cta Leu ga a Glu ctc Le u ttt Ph e g cg caa Gin Arg ct 9 Le u cct Pro ca a Gin 75 gt g Val tcc Ser ggc aaa Ly s att Ile t gg Trp gcg Ala gtc Val gg t Gly aac Asn gt g qc t Ala gtg Val1 tcg Ser att Ile gtg Val gcc Ala ccg Pro ggt ggC Gi y ctg Leui att Ile tta Leu tgg Trp gct Ala ctg Leu 110 gct Arq 15 ttt Phe ctg Leu gcc Ala caa Gin ttg Leui 95 gcg Ala gct gtg Val1 gtc Val gt C ValI tcc Se r at c Ile 80 gg C Gly gaa Giu gcg 1 cct Pro gcg Al a gqc Gly qg t Gi y cgc Arg gig Val ccg Pro gtg gg t Gly ct g Leu caa Gin ccg Pro atg Met gcc Ala t cg Ser aic ata Ile gcg Ala acc Thr gg t Gly cgc Arg gcg Al a 100 atc Ile ttt gct Ala atc Ile atc Ile gc~g Ala atc Ile ctg Leu gg C Gly aac 115 163 211 259 307 355 403 451 499 WO 01/00805 WO 0100805PCTLBOOIOO926 Val Thr Ser Giy Ala Gly Val Giy Ala Ala Ala Val Ile Val Phe Asn 120 125 130 ctg aca ttt ttq ggc aca tcc acc gtc qca gtc ggc gcg ttt att acc 547 Leu Thr Phe Leu Gly Thr Ser Thr Val Ala Val Gly Ala Phe Ile Thr 135 140 145 gcg gtg atc acc acg att ttg gta tat cag ctg gcc aga agc cgt gga 595 Ala Val Ile Thr Thr Ile Leu Val Tyr Gin Leu Ala Arg Ser Arg Gly 150 155 160 165 cgt gtg caa gtg atc aac ctg atc ctg aca ggc ata gcc att aat gca 643 Arg Val Gin Val Ilie Asn Le Ile Leu Thr Giy Ile Ala Ile Asn Ala 170 175 180 gta tcc ggc gcg ctg acc tca gtt ctt gat cta cat cgc gcc gac gag 691 Val Ser Gly Ala Leu Thr Ser Val Leu Asp Leo His Arg Ala Asp Giu 185 190 195 ctc ccg cga aga aat att ttc tgc aga tgq gtt cet cac ggc tcg cat 739 Leo Pro Arg Arg Asn Ile Phe Cys Arg Trp Val Pro His Gly Ser His 200 205 210 ggc gca cgt caa cgt 754 Gly Ala Arg Gin Arg 215 <210> 566 <211> 218 <212> PRT <213> Corynebacteriun giutamicom <400> 566 Met Val Val Lys Giu Val Asp Val Giu Lys Gin Lys Ala Gly Arg Val 1 5 10 Pro Gly Ala Ile Ala Lys Arq Arg Thr Val Arg Ile Val Leu Phe Val 25 Ala Leu Gly Ala Ile Val Ile Ala Ala Ser Leu Trp Ser Ile Leu Val 40 Gly Gin Tyr Thr Ile Pro Ile Arg Asp Leo Pro Ala Ile Leu Ala Ser 55 Gly Pro Thr Gly Ala Gin Thr Met Ala Glu Gin Val Val Trp Gin Ile 70 75 Arg Met Pro Arg Ile Val Leu Gly Leu Leo Val Gly Ala Ala Leu Gly 90 Val Ala Gly Ala Leu Leu Gin Ala Val Phe Ser Asn Pro Leu Ala Giu 100 105 110 Pro Ser Ile Ile Gly Val Thr Ser Gly Ala Gly Val Gly Ala Ala Ala 115 120 125 Val Ile Val Phe Asn Leo Thr Phe Leu Gly Thr Ser Thr Val Ala Val 130 135 140 WO 01/00805 Gly Ala Phe Ilie Thr Ala Val Ile Thr Thr Ile 145 150 155 Ala Arg Ser Arg Gly Arg Val Gin Val Ile Asn 165 170 Ile Ala Ile Asn Ala Val Ser Gly Ala Leu Thr 180 185 His Arg Ala Asp Giu Leu Pro Arg Arg Asn Ile 195 200 Pro His Gly Ser His Gly Ala Arg Gin Arg 210 215 <210> 567 <211> 357 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(334) <223> RXN02979 <400> 567 ctaggtcaag gaacttcact cggctagtcc ttagactcaa gcaccgtaag gcacgaaagt taccgaaagg actggttccc PCTIBOO/00926 Leu Val Tyr Gin Leu 160 Leu Ile Leu Thr Gly 175 Ser Val Leu Asp Leu 190 Phe Cys Arg Trp Vai 205 atgtgttcag acaaacactg atg acc gcc cca aac act ctc aag caa aca act ctt cgct Thr Leu Lys Gin Thr Thr Leu Arg! tgt gtc tcc aag att gaa aac aaaI Cys Vai Ser Lys Ile Giu Asn Lys aat gca gag qtg aag ttc tcc tcc Asn Ala Giu Vai Lys Phe Ser Ser 45 ccc agc aag gtc tct atc aag gat Pro Ser Lys Vai Ser Ile Lys Asp 60 qgc tac acc gca aag cca tca gca Gly Tyr Thr Ala Lys Pro Ser Ala tta <210> 568 <211> 78 <212> PRT <213> Corynebacterium giutamicum Met Thr Ala Pro Asn 1 gat gag ttc tct tgc cca tcc Asp Giu Phe Ser Cys Pro Ser 15 aat gga ttg gat ggc gtc gac Asn Gly Leu Asp Giy Val Asp aga atc ctt gtt gat cac gac Arg Ile Leu Val Asp His Asp gtc gct gca gtc gca gag gtt Val Ala Ala Val Ala Giu Val taaaactctc agttagacca WO 01/00805 <400> 568 Met Thr Ala Pro Asn Thr Leu Lys Gin Thr Thr 1 5 10 Phe Ser Cys Pro Ser Cys Val Ser Lys Ilie Glu 25 Leu Asp Gly Val Asp Asn Ala Glu Val Lys Phe 40 Leu Val Asp His Asp Pro Ser Lys Val Ser Ilie 55 Ala Val Ala Glu Val Gly Tyr Thr Ala Lys Pro 70 <210> 569 <211> 357 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(334) <223> RXN02987 <400> 569 gttgtttgat ccaggtcaag gaattaaccc ggaaaggacc acaggaacat gacgataaaa gatgaaagga cctggttacg PCT/tBOO/00926 Leu Arg Ser Asp Glu Asn Lys Leu Asn Gly Ser Ser Gly Arg Ile Lys Asp Leu Val Ala Ser Ala Ile gtatctttaa aggtgcaagc atq acc gcc ccc gcc acg ctg aag aac acc acc ttg cgc t Thr Leu Lys Asn Thr Thr Leu Arg S tgt gtc gcc aag atc qaa aac aag c Cys Val Ala Lys Ile Glu Asn Lys 1 aat gcg gag gtg aag ttc tcc tcc c Asn Ala Glu Val Lys Phe Ser Ser C 45 cca cag aag gtc tcc gta cgt gac c Pro Gin Lays Val Ser Val Arg Asp I 60 qgt tac acc gcc aag ccg tcg gcg E Gly Tyr Thr Ala Lys Pro Ser Ala 1 aag <210> 570 <211> 78 <212> PRT <213> Corynebacterium glutamicum Met Thr Ala Pro Ala 1 gat gag ttc acc tgt ccg agc Asp Glu Phe Thr Cys Pro Ser 15 aat ggt ttg gac ggc gtg gag Asn Gly Leu Asp Gly Val Glu cgc atc ctg atc acc cac gac Arg Ilie Leu Ile Thr His Asp gtc acc gcg gta gcc gag gtc Val Thr Ala Val Ala Glu Val tgacgcactc ccgaccccac WO 01/00805 <400> 570 Met Thr Ala Pro Ala Thr Leu Lys Asn Thr Thr 10 Phe Thr Cys Pro Ser Cys Val Ala Lys Ilie Giu 25 Leu Asp Gly Val Giu Asn Ala Giu Val Lys Phe 40 Leu Ilie Thr His Asp Pro Gin Lys Val Ser Val 55 Ala Val Ala Giu Val Gly Tyr Thr Ala Lys Pro 70 <210> 571 <211> 1041 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1018) <223> RXN03084 <400> 571 ccacccccac gtctaagttt tcccctattt acacacacct PCTJIBOO/00926 Leu Arg Ser Asp Giu Asn Lys Leu Asn Gly Ser Ser Gly Arg Ile Arg Asp Leu Val Thr Ser Ala Ile gaccgaagct gtaaggtttg cctaatcttt ttcaatctaa agtcagqata ttcacagccc atg tca tct cgc aga aaa Lys act Th r act Thr cct Pro qCC Ala ggt Gly gtt Val1 t cc Ser tgt Cys gaa Giu cg c Arg gga Gly cct Pro gag Glu tc c Se r tcc Ser ttc Phe gtc Val atc Ilie gca Ala cca Pro atc Ile ica Ser g ct Ala ctC Leu gtg Val1 Gly gaa Glu g tg Val gaa Gi u cac His 45 gag Glu ggt Gly gtc Val gcg Ala ctt Leu gag Glu 30 gga Gly cca Pro qca Ala gat Asp atc Ile Ctc gca Leu Ala gaa gca Glu Ala aca acc Thr Thr ctt gag Leu Giu gct gtc Ala Val 80 gga atc Gly Ile gct gct Ala Ala Met Ser Ser Arg Arg 1 gca gca cta cct ctt Ala Ala Leu Pro Leu tcc acc agc tct gcg Ser Thr Ser Ser Ala gaa gtt ccc gaa aat Glu Val Pro Glu Asn cta gac acc gca atc Leu Asp Thr Ala Ile gcc aac aac gtc act Ala Asn Asn Val Thr gag cct gtc ggc acc Giu Pro Val Gly Thr 100 ctc gag ccc gac ctg Leu Glu Pro Asp Leu wo 01/00805 WO 0100805PCT/EBOO/00926 atc Ile tcc Ser gac Asp 150 gag Gi u gag Giu gag Giu ttg Leu ctc Leu 230 tac Tyr ga a Gi u gat Asp gtc Val1 ctg Leu a tc Ile 135 aat Asn gat Asp cat His caa Gin gag Giu 215 ca g Gin gtc Vali gt a Vali acc Th r t tg Leu gg C Gly 120 gcc Ala gtc Vali ctc Le u gat Asp acc Thr 200 tgc Cys qcc Aia ttt Phe atc Ile agc Ser 280 gaa G iu 105 acc gat tcc Thr Asp Ser cca aco gtg Pro Thr Val gtc ttc atc Val Phe Ilie 155 atc caa ggc Ile Gin Giy 170 gtc gaa ggt Vai Giu Giy 185 atg agc cta Met Ser Leu gca gga ctc Aia Gly Leu gac atc gct Asp Ilie Aia 235 gtc acc gca Val Thr Ala 250 cga gaa aac Arg Giu Asn 265 tac tgg gta Tyr Trp Vai gac atc gat Asp Ilie Asp cgc Arg ttc Ph e 140 ggC Gly ttt Ph e aag Ly s tac Tyr acc Thr 220 cct Pro act Thr cgc Arg tct Ser ca c His 125 a tg Met gat Asp aat Asn acc Thr ggc Giy 205 att Ile gag Giu ga t Asp gaa Giu Giy 285 110 gcc Ala aca Thr gca Ala gac Asp gt C Vali 190 c cg Pro cct Pro aac Asn gtC Val cag Gin 270 g tc Val gaa Giu acc Thr t tg L eu aag Lys 175 aac Asn acc Thr gat Asp ttc Ph e acc Thr 255 ttC Phe gg t Giy atc Ile cat His ggc Gly 160 tgc Cys atg Met tca S er cag Gin atg Met 240 ga t Asp cca Pro cca Pro tac gac Tyr Asp 130 gtt gat Val Asp 145 aag aag Lys Lys gaa gag Giu Giu att cgt Ile Arg ttt gcC Phe Ala 210 gaa tgg Giu Trp 225 ctC gcc Leu Aia gaa aat Giu Asn tca ctc Ser Leu ctt ggc Leu Giy 290 115 cgc Arg c cg Pro cag Gin atc Ile

CCC

Pro 195 ggc Giy aag Lys acc Thr gag Giu acc Thr 275 ggc Gly Ct C Leu tgg Trp ga a Giu aag Lys 180 cgc Arg agc Ser gat Asp gcc Aia ctc Leu 260 ctt Leu agc S er gaa Giu a aa Ly s tcc Ser 165 tcc Ser gac Asp tct Ser ga c Asp ga c Asp 245 ccc Pro gt c Vai aaa Lys 499 547 595 643 691 739 787 835 883 931 979 1028 1041 gcc ttc ctc gac gca cag caa taatgtccac Aia Phe Leu Asp Ala Gin Gin 295 300 305 agctctcccc gat e210> 572 <211> 306 <212> PRT <213> Corynebacterium giutarnicum <400> 572 Met Ser Ser Arg Arg Lys Leu Ser Ser Ala Leu Ilie Val Leu Leu Aia 1 5 10 WO 01/00805 WO 0100805PCT/IBOO/00926 Ala Ser Glu Le u Al a Giu Le u T yr Val1 145 Lys Glu Ile Phe Glu 225 Leu Glu Ser Ala Thr Val1 Asp Asn Pro Glu Asp 130 Asp Lys Glu Arg Ala 210 T rp Ala Asn Leu Le u Ser Pro Thr Asn ValI Pro 115 Arq Pro Gin Ile Pro 195 Giy Lys Thr Glu Thr 275 Pro Ser Giu Ala Val Gly 100 Asp Leu Trp Giu Lys 180 Arg Ser Asp Ala Leu 260 Leu Leu Thr Ala Cys Ser Ser Ser Ser Giu Glu Glu Ala 25 Ala Asn Ilie Thr Th r Le u Giu Ly s Se r 165 Ser Asp Se r Asp Asp 245 Pro Val Thr Pro Ala 70 Gly Val1 Ile Ser Asp 150 Giu Glu Glu Leu Leu 230 Tyr Gi u Asp Arg Gin 55 Leu Ile Ser Leu Ile 135 Asn Asp His Gin Glu 215 Gin ValI ValI Thr Gi u 40 Arg Gly Pro Glu Gly 120 Ala Val1 Le u Asp Th r 200 Cys Ala Ph e Ile Ser 280 Ph e Val1 Ilie Al a Pro 105 Th r Pro Val1 Ile Val 185 Met Ala Asp Val Arg 265 Tyr Thr Val1 Th r Tyr 90 As n Asp Thr Phe Gin 170 Giu Ser Gly Ilie Thr 250 Giu Trp Asp Val Pro 75 Le u Ilie Ser Val Ile 155 Giy Giy Leu Leu Al a 235 Ala Asn Val Ala Leu Val Gi y Glu A r c Phe 140 Giy Phe Lys Tyr Thr 220 Pro Th r Arg Ser His Glu Gly Val1 Ala His 125 Met Asp Asn Th r Gly 205 Ile Giu Asp Giu Gly 285 Giy Pro Ala Asp Ile 110 Ala Thr Ala Asp Val1 190 Pro Pro Asn Val Gin 270 Val1 Thr Thr Leu Giu Ala Val Gly Ile Ala Ala Glu Ile Thr His Leu Gly 160 Lys Cys 175 Asn Met Thr Ser Asp Gin Phe Met 240 Thr Asp 255 Phe Pro Gly Pro Leu Gly Gly Ser Lys Val Leu Giu Asp Ile Asp Ala Phe Leu Asp Ala zvu Gin Gin 305 <210> 573 <211> 440 WO 01/00805 PCTJIBOO/00926 <212> DNA <213> Corynebacterium glutarnicum <2 <221> CDS <222> (417) <223> RXN03183 <400> 573 gaa gcc gaa gca acc gca ggc aaa ttc gaa gta cag ccc ctc gta ggt 48 Glu Ala Glu Ala Thr Ala Gly Lys Phe Giu Val Gin Pro Leu Val Gly 1 5 10 aaa gac ggc gtc ggc gta tec acc ctt gqt ggc tac aac aac ggc atc 96 Lys Asp Gly Val Gly Val Ser Thr Leu Gly Gly Tyr Asn Asn Gly Ile 25 aac gtc aac tcc gaa aac aag gca acc gcc cgc gac ttc atc gaa ttc 144 Asn Val Asn Ser Glu Asn Lys Ala Thr Ala Arg Asp Phe Ile Glu Phe 40 atc atc aac gaa gag aac caa acc tgg ttc gcg gac aac tcc ttc cca 192 Ilie Ile Asn Giu Giu Asn Gin Thr Trp Phe Ala Asp Asn Ser Phe Pro 55 cca qtt ctg gca tcc atc tac qat gat gag tcc ctt gtt gag cag tac 240 Pro Val Leu Ala Ser Ilie Tyr Asp Asp Glu Ser Leu Val Glu Gin Tyr 70 75 cca tac ctg cca gca ctg aag gaa tcc ctg gaa aac gca gca cca cgc 288 Pro Tyr Leu Pro Ala Leu Lys Giu Ser Leu Glu Asn Ala Ala Pro Arg 90 cca gtg tct cct ttc tac cca gcc atc tcc aag gca ato cag gac aac 336 Pro Val Ser Pro Phe Tyr Pro Ala Ile Ser Lys Ala Ilie Gln Asp Asn 100 105 110 gcc tac gca gcg ctt aac ggc aac gtc gac gtt gac cag gca acc acc 384 Ala Tyr Ala Ala Leu Asn Gly Asn Val Asp Val Asp Gin Ala Thr Thr 115 120 125 gat atg aag gca gcg atc gaa aac gct tcc agc tagttcggta atttagttca 437 Asp Met Lys Ala Ala Ile Giu Asn Ala Ser Ser 130 135 ttc 440 <210> 574 <211> 139 <212> PRT <213> Corynebacterium glutamicun <400> 574 Glu Ala Giu Ala Thr Ala Gly Lys Phe Giu Val Gin Pro Leu Val Gly 1 5 10 Lys Asp Gly Val Gly Val Ser Thr Leu Gly Gly Tyr Asn Asn Gly Ile 25 Asn Val Asn Ser Glu Asn Lys Ala Thr Ala Arg Asp Phe Ile Glu Phe PCTJIBOO/00926 WO 01/00805 40 Ile Ile Asn Giu Giu Asn Gin Thr Trp Phe Ala Pro Val Leu Ala Ser Ile Tyr Asp Asp Giu Ser 70 75 Pro Tyr Leu Pro Ala Leu Lys Giu Ser Leu Giu 90 Pro Val Ser Pro Phe Tyr Pro Ala Ile Ser Lys 100 105 Ala Tyr Ala Ala Leu Asn Giy Asn Vai Asp Val 115 120 Asp Met Lys Ala Ala Ilie Giu Asn Ala Ser Ser 130 135 <210> 575 <211> 1077 <212> DNA <213> Corynebacteriun giutarnicum <220> <221> GDS <222> (1054) <223> RXN01139 <400> 575 accaccgtgq caggcttttt cagtggcaag aagattaatt taagctttca ataaggggaq aagtgtgctt aqctggggtc Asn Ser Phe Pro Val Giu Gin Tyr Ala Aia Pro Arg Ile Gin Asp Asn 110 Gin Ala Thr Thr 125 ctt Leu cat His tt Phe gcc Ala a tg Met att Ile cag Gin ga c Asp gcg Ala 9gc Gly ctg Leu 99c Gi y ca g Gin gg a Gly gtc Val1 ctt Leu tcc Ser cg t Arg cgc Arg ctc Le u atc Ile at Ile gac Asp cgg Arg agt Ser gga Gly att Ile tcc Ser tcc Se r gca Ala tat Tyr cac His t tc Phe gga Giy act Thr 75 cgc Arg 9Cg Ala t ca Ser acc Thr tct Ser 60 9gc Giy act Th r aat As n gct Ala 30 atc Ile g ca Al a atc Ile cg t Arg gat Asp ag c Ser ttc Ph e ctg Leu 9cg Al a 80 acc Thr ctccacccct tcattttcaa aig gaa agc cac gat 115 Met Glu Ser His Asp 1 ggc cac gac cac agc 163 Gly His Asp His Ser.

icc ctc aag gct ctt 211 Ser Leu Lys Ala Leu cia gcq gaa cta atc 259 Leu Ala Glu Leu Ile gct gac gcc aig cac 307 Ala Asp Ala Met His gct gtc gcc atg ctc 355 Ala Val Ala Met Leu tac gga tac aag cgt 403 Tyr Gly Tyr Lys Arg 100 WO 01/00805 WO 0100805PCT/EBOO/00926 gcg Ala tct Ser gaa Glu acc Thr 150 atc Ile tcc Ser atg Met cct Pro cgt Arg 230 aaa Lys gac Asp aca Th r cta Leu ga t Asp ga a Gi u gtg ValI atc Ile 135 aac Asn aa t Asn gt t Val1 ccg Pro cgc Arg 215 gt t Val gtc Val1 gg c Gly aat Asn tcc Ser 295 gtc Val1 tqg Trp 120 ca g Gin gga Gly atg Met g cc Ala gcc Ala 200 gca Ala cct Pro cca Pro aag Lys cag Gin 280 aaa Lys ttg Leu 105 atc Ile acc Th r att Ilie cg t Arg gt c ValI 185 ga t Asp ttc Phe aca Thr ggt Gly gaa Giu 265 ct g Leu ctt Leu gca gcg atg gtt Ala Ala Met Val aac 9CC Asn Ala acc gtt gtt aca gca ctg Thr Val Vai Thr Ala Leu gtc Val1 aac Asn t cc Ser gga G ly 170 att Ile acc Thr agc Se r g gt Gly gtc Val 250 atc Ilie cat His 9 gg Gly gt t Val ctg Leu gcc Ala 155 gca Ala att Ile att Ile ctc Leu g cg Ala 235 a gc Ser ctg Le u ag t Ser atc Ile gag Glu atg Met 140 ctg Leu ttc Phe gcg Ala gct Ala ctg Leu 220 gag Giu ga t Asp gcc Ala tqt C ys ttg Leu 300 gcc Ala 125 ctc Leu gtg Val1 ctt Le u qqc Gly tcg Se r 205 aag Lys ct Pro gtg Val acg Thr ggc Gly 285 cac H is atc Ile atc Ilie ttg Leu cac His ctg Leu 190 att Ilie gaa Giu gca Al a cat His gt g Val 270 gtg Val1 t ca Ser a tg Met gtc Val atg Met gt t Vai 175 gtg Val gcg Ala gc t Ala gag Glu gat Asp 255 cat His ttg Leu a ca Thr cg t Arg gcg Ala cgc Arq 160 ctc Leu att Ilie att Ile ctc Le u gtc Val 240 ctt Le u ttg Leu gat Asp att Ile ctg Leu gtc Val1 145 cac His agt Ser cg c Arg gct Ala aat Asn 225 gac Asp cac His gtg Val1 Arg cag Gin 9gC Gly 130 att Ile ca a Gin ga c Asp tac Tyr gcg Ala 210 atc Ile g ca Ala att Ile gtg Val gca Ala 290 ctg aag Lys ggt Gly gat Asp a tg Met acg Thr 195 att Ile t tq Leu gcc Ala tgg Trp ga t Asp 275 ga a Giu gaa ga c Asp ttt Phe gci Gly ctg Leu 180 gga Gly att Ile ttg Leu ct t Leu agc Ser 260 t cg Ser gcg Ala agc ctg Leu gtC Val aa t Asn 165 qg t Gly tggi Trp att Ile gag Giu 09 t Arg 245 att Ile tct Ser gaa Giu gca 451 499 547 595 643 691 739 787 835 883 931 979 1027 1074 1077 Leu Glu Ser Ala cac agt gat cat gaa agt His Ser Asp His Giu Ser gtg tgc tgatatagag tatgtcccat Val Cys 310 999 <210> 576 WO 01/00805 <211> 318 <212> PRT <213> Corynebacterium g <400> 576 Met Glu Ser His Asp Leu PCT/IB00/00926 lutamicum 1 Gly Ser Leu Ala Ala Tyr Val Leu Val 145 His Ser Arg Ala Asn 225 Asp His Val Arg His Leu Ala Asp Val Gly Val Gly 130 Ile Gin Asp Tyr Ala 210 Ile Ala Ile Val Ala 290 Asp Lys Glu Ala Ala Tyr Thr 115 Lys Gly Asp Met Thr 195 Ile Leu Ala Trp Asp 275 Glu His Ala Leu Met Met Lys 100 Ala Asp Phe Gly Leu 180 Gly Ile Leu Leu Ser 260 Ser Ala 5 Ser Leu Ile His Leu Arg Leu Leu Val Asn 165 Gly Trp Ile Glu Arg 245 Ile Ser Glu His Phe Ala Met 70 Ile Ala Ser Glu Thr 150 Ile Ser Met Pro Arg 230 Lys Asp Thr Leu Gin Asp Ala Gly 55 Leu Gly Glu Val Ile 135 Asn Asn Val Pro Arg 215 Val Val Gly Asn Ser 295 Gin Arg Gly Leu 25 Val Ile 40 Leu Ile Ser Asp Arg Arg Val Leu 105 Trp Ile 120 Gin Thr Gly Ile Met Arg Ala Val 185 Ala Asp 200 Ala Phe Pro. Thr Pro Gly Lys Glu 265 Gin Leu 280 Lys Leu Ser Tyr 10 Gly His Ile Phe Ser Gly Ser Thr 75 Ala Arg 90 Ala Ala Val Val Asn Leu Ser Ala 155 Gly Ala 170 Ile Ile Thr Ile Ser Leu Gly Ala 235 Val Ser 250 Ile Leu His Ser Gly Ile Ala Ser Thr Ser Gly Thr Met Glu Met 140 Leu Phe Ala Ala Leu 220 Glu Asp Ala Cys Leu 300 His Asn His Ala Ser Ile Leu Ala Leu Ile Ser Arg Val Asn 110 Ala Ile 125 Leu Ile Val Leu Leu His Gly Leu 190 Ser Ile 205 Lys Glu Pro Ala Val His Thr Val 270 Gly Val 285 His Ser Pro Pro Ile Leu Ile Ala Ala Met Val Met Val 175 Val Ala Ala Glu Asp 255 His Leu Thr Asp Ser Phe Leu Ala Thr Thr Arg Ala Arg 160 Leu Ile Ile Leu Val 240 Leu Leu Asp lie WO 01/00805 Gin Leu Glu Ser Ala Asp His Ser Asp His Glu Ser Val Cys 305 310 315 <210> 577 <211> 2733 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(2710) <223> RXN00378 <400> 577 accgtgagcc ttatactgtg aggacattaa aaqtgacacc tctttttcta PCTIIBOO/00926 tcttttacaa cccaagaagg tttatcgtga gcacaccgga ttcttcctcg aac Asn 9ga Gly a gc Ser cc a Pro t cc Ser ga t Asp atc Ile ctc Leu cg a Arg caa Gin 150 act Th r ttg Leu att Ilie t cg Ser gcg Aia Arg aaa L ys agc Ser atg Met 135 gaa Glu gct Al a aac Asn aag Lys ttg Leu gat Asp gta Val1 gca Aia g cg Ala 120 qat Asp a tg Met atc Ile cga Arg qt t Vali atg Met ctg Le u cat His cac His 105 qa a Giu gcc Ala gta Val1 tct gac Ser Asp ccc acc Ala Thr tct ttg Ser Leu gtg gtg Val Val att tac Ile Tyr 75 ccc gaa Pro Giu ctt tct Leu Ser gta aat Val Asn aag cga Lys Arg cat gcg His Ala 155 gc c Al a gcc Ala gcc Ala ga a Giu 60 cct Pro acc Th r aat As n agg Arg atc Ile 140 gta Val1 aaa Lys tca Se r ttt Ph e 45 aaa Lys tca Ser ctc Leu tca Ser gaa Glu 125 t cg Ser cgc Arg aca Thr gaa Giu 30 gag Giu g cc Al a caa Gin att Ile t cg Ser 110 gca Ala cc t Pro gct Ala gcg Ala 15 gaa Giu ctc Leu cta Leu act Th r gag Glu 95 ct g Leu cgc Arg cg t Arg cgt Arg g cg Ala gag Glu gag Glu gag Giu gca Al a 80 gtg Val1 ctq Leu ctt Le u gtg Val gaa Giu 160 gtg gac Val Asp 1 ctc aag Leu Lys gaa gat Giu Asp ggg tta Gly Leu aag atc Lys Ile tgg att Trp Ile ttt gag Phe Glu cgc agg Arg Arg gat cgt Asp Arg 130 cga agg Arg Arg 145 agt ggt Ser Gly g ca Ala tta Leu agc Ser ccc Pro aca Thr ca g Gin cat His 115 tac T yr cat His tgg Trp ggt Gly agc Ser aat Asn ggt Giy gca Ala ttc Phe 100 caa Gin cgc Arg aac Asn att Ile aag gcc gta Lys Ala Val gtt Val t ca Se r gca Ala gt a Val act Thr ggc Gly cag Gin t cc Ser cga Arg aaa Lys 165 115 163 211 259 307 355 403 451 499 547 595 WO 01/00805 WO 0100805PCTIBOO/00926 CgC Arg gtg Val1 t cg Ser tgg Trp gtg Val 230 att Ile gct Al a gga Gly ggc Gi y acc Th r 310 tcc Ser att Ile ctg Leu cca Pro t cg Ser 390 agg Arq ctg Le u ttg Le u cag Gin 215 gtg Val cgt Arg gct Al a gg t Gly a cg Thr 295 gtg Val1 agt Ser gtc Val a ac Asn cct Pro 375 cgg Arg aat As 0 ttc Phe ccg Pro 200 ttt Phe gtg Val1 cga Arg g ca Al a aaa Lys 280 t tg Leu t tq Leu t tg Leu act Thr att Ile 360 aat Asn atc Ile cac His acc Thr 185 ttt Phe ga t Asp tgg Trp gga Gly tac T yr 265 tcc Ser acc Thr ctt Leu tta Leu gtg ValI 345 cca Pro acc Thr gca Ala acc Thr 170 gCC Ala gcg Ala tat Tyr ggt Gly att Ile 250 g cg Ala tgg Trp caa Gin ctt Leu gcg Aia 330 gtg Val1 gtg Val a cg Thr gc t Ala acc Thr cgc Arg Ct C Leu tgg Trp qcc Al a 235 t cc Ser tgg Trp cgg Arg aac Asn qcc Ala 315 qaa Glu cg t Arg cag G in atc Ile agc Ser 395 t cg Ser gca Al a atc Ile cag Gin 220 tgg Trp gct Ala tct Ser tca Ser gag Glu 300 gga Gly ctt Leu aaa Lys gaa Glu cct Pro 360 atc Ile cag Gin ctg Leu gta Val 205 t gg T rp C cg Pro ctt Leu atc Ile tat Tyr 285 att Ile Arg ggt Gly cac His gtc Val 365 gtg Vali atc Ile cat His att Ile 190 t tg Leu ttg Leu ttt Phe ga t Asp gcc Ala 270 ccg Pro tat Tyr ctg Leu cqc Arg Cga Arg 350 cg t Arg ga t Asp atg Met gaa Giu 175 aca Thr gcg Ala tcc Ser cac His gcg Ala 255 a tq Met tcc Ser ttt Phe ctg Leu ctC Leu 335 t tg Leu gtC Val qgC Gly gga Gly gac Asp CC t Pro tta Leu gc t Ala cgc Arg 240 acc Thr ctg Le u tgg Trp ga t Asp aca Thr 320 caa Gin aag L ys aat Asn act Th r caa Gin 400 C ca Pro aag Lys t cg S er qtg Val 225 gct Ala ag C Ser ttg Leu ttc Ph e qtg Val1 305 agg Arg atc Ile cg C Arg gac Asp gtc Val1 385 qac Asp atg Met cg t Arg t tg Leu 210 ttg Leu gca Ala t ca Ser ttt Phe qct Ala 290 gcc Ala cgt Arg ga t Asp gt a ValI ga t Asp 370 atc Ile ca q Gin t cg Ser t tq Leu 195 aat Asn gct Al a qca Ala atc Ile gaa Giu 275 ttt Ph e tgc Cys cga Arg c ca Pro gtc Val1 355 gtg Val1 gt Gly cg t Arg ggc gat Gly Asp 180 tgg gtg Trp Val cct tcg Pro Ser att Cct Ile Pro ggc ggt Gly Gly 245 gct att Ala Ilie 260 acc cca Thr Pro gac cac Asp His gga atc Gly Ile agc caa Ser Gin 325 cag cgc Gin Arg 340 cag gaa Gin Giu aaa gtt Lys Vai qgc ggt Gly Gly gat gta Asp Val 405 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 WO 01/00805 PCTArBOOIOO926 aaa gta aat qac aaa gtt ttc 9cc ggc agc ctc aac ctc gaa tcc gaa 1363 Lys Val Asn Asp Lys Val Phe Ala Gly Ser Leu Asn Leu Glu Ser Glu 410 415 420 atc aag gtt cgt gtt att cgc act ggt cac cgc acc cgc atc gcc gcg 1411 Ilie Lys Val Arg Val Ilie Arg Thr Gly His Arq Thr Arg Ile Ala Ala 425 430 435 gta cat agg tgg gtt aaa gaa gcg acg ttg aag gaa aac cgc cac aat 1459 Val His Arg Trp Val Lys Glu Ala Thr Leu Lys Glu Asn Arg His Asn 440 445 450 agg gca gcg atc cgt tcg gcc ggt aac ctt gtg ccc atc acg ttc acc 1507 Arg Ala Ala Ile Arg Ser Ala Gly Asn Leu Val Pro Ile Thr Phe Thr 455 460 465 ctt gct gtg gtg gac ttc tgt ctg tgg gca ctg atc tct gga aac atc 1555 Leu Ala Val Val Asp Phe Cys Leu Trp Ala Leu Ilie Ser Gly Asn Ile 470 475 480 485 aac gct gca ttt acc act acc ttg gct gtc ctt gcg tgc gtg gct ccg 1603 Asn Ala Ala Phe Thr Thr Thr Leu Ala Val Leu Ala Cys Val Ala Pro 490 495 500 gtg gcc tta gcg ttg tct gct cca ctt gcc acg agg aat tcc atc gaa 1651 Val Ala Leu Ala Leu Ser Ala Pro Leu Ala Thr Arg Asn Ser Ile Glu 505 510 515 gct gca gca cga cac ggt att ttg gtc cgc tct ggt gaa att ttc cga 1699 Ala Ala Ala Arg His Gly Ilie Leu Val Arg Ser Gly Glu Ile Phe Arg 520 525 530 gtt ctc gat gat gtg gat act gcc gta ttt aat cgt gtg ggc aca cta 1747 Val Leu Asp Asp Val Asp Thr Ala Val Phe Asn Arg Val Gly Thr Leu 535 540 545 acc gat ggc gaa atg aca gtg gaa acc gtc aca gca gac aaa ggc gag 1795 Thr Asp Gly Glu Met Thr Val Glu Thr Val Thr Ala Asp Lys Gly Glu 550 555 560 565 gac cca gaa cta gtg ctq cgt gtc gcc ggg gcg ttg gcc atg gaa tcc 1843 Asp Pro Glu Leu Val Leu Arg Val Ala Gly Ala Leu Ala Met Glu Ser 570 575 580 cac cac gcg att tcc aaa gca ctg gtg aaa gca tcc cgt gaa gct cgt 1891 His His Ala Ile Ser Lys Ala Leu Val Lys Ala Ser Arg Glu Ala Arg 585 590 595 qat acc ggc gcc ggt ggt gaa gat gtc cca cac tgg att qaa gta ggc 1939 Asp Thr Gly Ala Gly Gly Glu Asp Val Pro His Trp Ile Giu Val Gly 600 605 610 aac gtg gaa atc acc gaa gcc ggc tca ttc caa gca acc atc gag ctg 1987 Asn' Val Glu Ilie Thr Glu Ala Gly Ser Phe Gin Ala Thr Ilie Glu Leu 615 620 625 cca ctg atc aaa cca tct ggc gaa aaa atc atg cgc acc aca gaa gca 2035 Pro Leu Ile Lys Pro Ser Gly Glu Lys Ile Met Arg Thr Thr Glu Ala 630 635 640 645 ctc ctg tgg cga cca cga tcc atg aca gaa gtc cgt gag cac tta agc 2083 wo 01/00805 WO 0100805PCTfIBOO/00926 Leu Leu ccc cga Pro Arg cga tgg Arg Trp aga tca Arg Ser 695 gag acc Glu Thr 710 gca gac Ala Asp aag aaa Lys Lys gcg atg Ala Met gtg ggt Val Gly 775 tcc gat Ser Asp 790 agc gtq Ser Val aat ggc Asn Gly ctt gca Leu Ala ctg gcg Leu Ala 855 Trp cta Leu aaa L ys 680 gat Asp atg Met agc Ser gcc Ala atc Ile 760 gta Val gac Asp ccg Pro aat Asn gtg Val1 840 t ct Ar g gtg Val1 665 ggc Gly t cc Ser atg Met tta Leu cag Gin 745 ggc Gly ctg Leu ccg Pro a cg Th r att Ile 825 tct Ser t cq Pro Arg 650 gca gca Ala Ala aaa gac Lys Asp tcc gat Ser Asp ctt tca Leu Ser 715 ggc atc Gly Ile 730 gtc gtc Val Vai gat gaa Asp Giu atg ggc Met Gly gca gct Ala Ala 795 ctg ttt Leu Phe 810 gct ctg Ala Leu ggc ttg Gly Leu ctg ctt Ser gca Ala cgc Arg gcg Ala 700 cg t Arg acc Thr cg t Arg tca Ser gtc Val1 780 gac Asp aaa Lys gcc Al a ctg Leu att Met acc Th r gga Gly 685 att Ile ga t Asp cac His gca Ala gta Val 765 gat Asp gtt Val ctg Le u t gg Trp cat His 845 gaa Thr tca Ser 670 gtt Val1 att Ile act Thr gtc Val1 gtc Val1 750 a tg Met cq t Arg gtg ValI gct Ala atc Ile 830 cca Pro Giu 655 ggt Gly atc Ile gcg Ala tac Tyr ttg Leu 735 cac His ga c Asp ccc Pro gtc Val1 cga Arg 815 tat Tyr atg Met Val1 Gly act Th r att Ile ccg Pro 720 gcc Ala acc Th r tgt Cys t ca Se r atg Met 800 cgc Arg aac Asn gct Aila Arg gca Ala cta Leu gaa Glu 705 gtg Val ggc Gly cg c Arg t tg Le u ga t Asp 785 cgc Arg tac Tyr gg t Gly gcg Ala 9gc G ly 865 Glu His cca ctg Pro .Leu 675 agt gac Ser Asp 690 gaa caa Glu Gin gca cgt Ala Arg atc gcg Ile Ala gga tcc Gly Ser 755 aaa gtc Lys Val 770 ctg cgt Leu Arg gaa gag Glu Glu gcc aag Ala Lys gtt gcc Val Ala 835 acc qtg Thr Val 850 Leu 660 atc Ile cac His ggc Gly cga Arg C cg Pro 740 act Thr gct Ala gat Asp g tC Val1 ttg Leu 820 atg Met gct Al a Se r gtg Val gtg Val1 a tc Ile tac Tyr 725 gg c Gly qtc Val1 gac Asp qa t Asp atg Met 805 gtc Val gtg Val atg Met 2131 2179 2227 2275 2323 2371 2419 2467 2515 2563 2611 2659 2707 2733 tgg cgc tcg Ser Ser Leu Leu Ile Giu Trp Arg Ser 860 agg gcq cgc aag Arg Ala Arg Lys tac taaccagcaa ttcccaagcc caa Tyr 870 <210> 578 WO 01/00805 PCT/IBOO/00926 -211> 870 ,<212> PRT <-213> Corynebacterium glutarnicum <400> 578 Val Asp Lys Ala Val Asn Thr 1 Le u Glu Gly Ly s Trp Phe Arg Asp Arg 145 Ser Pro L ys S er Val1 225 Ala Ser Le u Lys Asp Leu Ile Ile Giu Arg Arg 130 Arg Gly Met Arg Le u 210 Leu Ala Ser Phe Al a Leu Ser P~r o Thr Gln His 115 Tyr His Trp Ser Le u 195 Asn Ala Ala Ile Glu 275 Gly Ser Asn Gly Ala Phe 100 Gin Arg Asn Ile Gly 180 Trp Pro Ile Gly Al a 260 Thr 5 Val Gly Ser Ser Ala Pro Val Ser 70 Thr Asp Giy Ile Gin Leu Ser Arg Arg Gln 150 Lys Arg 165 Asp Val Val Ser Ser Trp Pro Val 230 Gly Ile 245 Ile Ala Pro Gly Leu Ile Ser 55 Ala Arg Lys Ser Met 135 Giu Arg Le u Leu Gin 215 Val1 Arg Ala Gly Thr 295 Ala Ile Ser 10 Asn Arg Ala 25 Lys Val Ser 40 Leu Met Val Asp Leu Ile Val His Pro 90 Ala His Leu 105 Ala Glu Val 120 Asp Ala Lys Met Val His Asn His Thr 170 Phe Thr Ala 185 Pro Phe Ala 200 Phe Asp Tyr Val Trp Gly Arg Gly Ile 250 Ala Tyr Ala 265 Lys Ser Trp 280 Leu Thr Gin Asp Ala Thr Ala Leu Ala Val Glu Tyr Pro 75 Glu Thr Ser Asn Asn Arg Arg Ile 140 Ala Vai 155 Thr'% Se'r Arg Ala Leu Ile Trp Gin 220 Ala Trp 235 Ser Ala Trp Ser Arg Ser Asn Giu 300 Lys Ser Ph e Ly s Ser Leu Se r Giu 125 Ser Arg Gin Leu Val1 205 Trp Pro Leu Ilie Tyr 285 Ile Thr Giu Glu Ala Gin Ile Ser 110 Ala Pro A la His Ile 190 Leu Le u Phe Asp Ala 270 Pro Tyr Ala Glu Leu Leu Thr Glu Leu Arg Arg Arg Giu 175 Th r Al a Ser His Ala 255 Met Se r Ph e Ala Giu Giu Glu Ala Vali Leu Le u Val1 Glu 160 Asp Pro Le u Ala Arg 240 Thr Leu Trp Asp Phe Ala 290 Phe Asp His Gly WO 01/00805 PCT/IB00/00926 Val 305 Arg Ile Arg Asp Val 385 Asp Asn Thr Glu Pro 465 Ile Ala Arg Gly Arg 545 Ala Leu Ser Trp Ala Arg Asp Val Asp 370 Ile Gin Leu Arg Asn 450 Ile Ser Cys Asn Glu 530 Val Asp Ala Arg Ile 610 Cys Arg Pro Val 355 Val Gly Arg Glu Ile 435 Arg Thr Gly Val Ser 515 Ile Gly Lys Met Glu 595 Glu Gly Ser Gin 340 Gin Lys Gly Asp Ser 420 Ala His Phe Asn Ala 500 Ile Phe Thr Gly Glu 580 Ala Val lie Gin 325 Arg Glu Val Gly Val 405 Glu Ala Asn Thr Ile 485 Pro Glu Arg Leu Glu 565 Ser Arg Gly Thr 310 Ser Ile Leu Pro Ser 390 Lys Ile Val Arg Leu 470 Asn Val Ala Val Thr 550 Asp His Asp Asn Val Ser Val Asn Pro 375 Arg Val Lys His Ala 455 Ala Ala Ala Ala Leu 535 Asp Pro His Thr Val 615 Leu Leu Thr Ile 360 Asn Ile Asn Val Arg 440 Ala Val Ala Leu Ala 520 Asp Gly Glu Ala Gly 600 Glu Leu Leu Val 345 Pro Thr Ala Asp Arg 425 Trp Ile Val Phe Ala 505 Arg Asp Glu Leu Ile 585 Ala Ile Leu Ala 330 Val Val Thr Ala Lys 410 Val Val Arg Asp Thr 490 Leu His Val Met Val 570 Ser Gly Thr Ala 315 Glu Arg Gin Ile Ser 395 Val Ile Lys Ser Phe 475 Thr Ser Gly Asp Thr 555 Leu Lys Gly Glu Gly Leu Lys Glu Pro 380 Ile Phe Arg Glu Ala 460 Cys Thr Ala Ile Thr 540 Val Arg Ala Glu Ala 620 Arg Gly His Val 365 Val Ile Ala Thr Ala 445 Gly Leu Leu Pro Leu 525 Ala Glu Val Leu Asp 605 Gly Leu Arg Arg 350 Arg Asp Met Gly Gly 430 Thr Asn Trp Ala Leu 510 Val Val Thr Ala Val 590 Val Ser Leu Thr 320 Leu Gin 335 Leu Lys Val Asn Gly Thr Gly Gin 400 Ser Leu 415 His Arg Leu Lys Leu Val Ala Leu 480 Val Leu 495 Ala Thr Arg Ser Phe Asn Val Thr 560 Gly Ala 575 Lys Ala Pro His Phe Gin WO 01/00805 WO 0100805PCTALBOO/00926 Ala Thr Ile Glu Leu Pro Leu Ile Lys Pro Ser Gly Glu Lys Ile Met 625 630 635 640 Arg Thr Thr Glu Ala Leu Leu Trp Arg Pro Arg Ser Met Thr Glu Val 645 650 655 Arg Glu His Leu Ser Pro Arq Leu Val Ala Ala Ala Thr Ser Gly Gly 660 665 670 Ala Pro Leu Ile Val Arg Trp Lys Gly Lys Asp Arg Gly Val Ilie Thr 675 680 685 Leu Ser Asp His Val Arg Ser Asp Ser Ser Asp Ala Ile Ile Ala Ile 690 695 700 Glu Glu Gin Gly Ile Giu Thr Met Met Leu Ser Arg Asp Thr Tyr Pro 705 710 715 720 Val Ala Arg Arg Tyr Ala Asp Ser Leu Gly Ilie Thr His Val Leu Ala 725 730 735 Gly Ilie Ala Pro Gly Lys Lys Ala Gin Val Val Arg Ala Val His Thr 740 745 750 Arg Gly Ser Thr Val Ala Met Ilie Gly Asp Glu Ser Val Met Asp Cys 755 760 765 Leu Lys Val Ala Asp Val Gly Val Leu Met Gly Val Asp Arg Pro Ser 770 775 780 Asp Leu Arg Asp Asp Ser Asp Asp Pro Ala Ala Asp Val Val Val Met 785 790 795 800 Arg Glu Glu Val Met Ser Val Pro Thr Leu Phe Lys Leu Ala Arg Arg 805 810 815 Tyr Ala Lys Leu Val Asn Giy Asn Ilie Ala Leu Ala Trp Ile Tyr Asn 820 825 830 Gly Val Ala Met Val Leu Ala Val Ser Gly Leu Leu His Pro Met Ala 835 840 845 Ala Thr Val Ala Met Leu Ala Ser Ser Leu Leu Ilie Glu Trp Arg Ser 850 855 860 Gly Arg Ala Arg Lys Tyr 865 870 <210> 579 <211> 1925 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(1902) <223> RXN01338 <400> 579 aaa act tat acc cca aat ccc tgg atg tta ttc atc cgc tca ttt gat 48 Lys 1 ggc Gly tta Leu ata Ile aaa Lys tcc Ser atc Ile Arg aca Thr atc Ilie 145 cat His a tg Met aaa Lys acg Th r gtt ValI 225 a tg Met WO 01/00805 Thr Tyr Thr atc atc act Ile Ile Thr tgg ctg gct Trp Leu Ala gcc atc gtt Ala Ile Val tca gcc att Ser Ala Ile ate att act Ilie Ilie Thr gtg ctc atg Val Lee Met 100 cga gcc agt Arg Ala Ser 115 gct cac cgc Ala His Arg 130 gcc gtg gaa Ala Val Gie gaa ctt tgt Glu Leu Cys gat gag tct Asp Glu Ser 180 ggt tcg caa Gly Ser Gin 195 att gtt gcc Ile Val Ala 210 ggt qta ctc Gly Val Leu gct gac cgt Ala Asp Arg Pro Asfl Pro Trp Met Leu Phe Ilie Arg Ser Phe Asp 5 10 PCTIBOOIOO926 gtc Val cta L eu atc Ile aaa Lys tct Ser ctc Leu ggc Gly ctg Leu gag Glu ccc Pro 165 tat Tyr gca Ala aca Thr cat His ctt Leu gca Ala gat Asp gta Val1 act Thr 70 gtg Val tct Ser acc Thr ttg Lee atc Ilie 150 gtg Val ctc Leu a tg Met aag Lys gaa Glu 230 99C Gly gc c Ala ct a Lee gg t Gly 55 cg c Arg t tg Le u ggt Gly ttg Lee gg t Gly 135 acg Th r gat Asp acg Th r t cg Ser ctt Lee 215 gca Ala gcg Ala ctt Lee ga t Asp 40 ggc Gly ggt Gly tta Leu ggt Gly gac Asp 120 gca Ala gtt ValI ggt Gly ggt Gly gg t Gly 200 gcc Ala gaa Glu t gc Trp gtt Val 25 ggc Gly att Ile Gly ggg Gly gaa Gie 105 gca Ala acc Th r ggt Gly ga a Gle gag Giu 185 g ca Ala cat His aac Asn at Tyr gcc Ala ctt Lee ccg Pro gcg Ala gag Gie 90 g cg Ala ctt Lee att Ile gat Asp atc Ile 170 ccc Pro gt c Va 1 ga t Asp aac Asn a cg Thr atc Ile gct Ala ttg Lee ga t Asp 75 tgg Trp cta Lee gcc Ala ctt Leu tta Lee 155 gtg ValI tat Tyr aat Asn tcc Ser cgc Arg 235 gca Ala aaa Lys atg Met act Th r t tg Lee gaa Glu Arg gat Asp 140 gtg Val1 gca Ala gt g Val ggt Gly aga Arg 220 cca Prc ata Ile aac Asn tg Trp tta Leu gt t Val1 gag Gie cgc Arg 125 gga Gly gcg Ala ggc Gly gtg Val gat Asp 205 tac T yr gaa Gle cat His tgg Trp gat Asp gca Ala gcc Al a gca Ala 110 gca Ala acc Thr g tg ValI cac His agt Ser 190 act Thr gcc Ala a tg Met ctc Lee cc t Pro gtg Val1 g ca Ala gcg Ala gca Ala cca Pro gaa Gle ct c Lee Gi y 175 aaa Lys ccg Pro caa Gin cg c Arg att Ile tta Lee ctg Lee gtc Val atc Ile t ca Ser ag t Ser gag Gie ccg Pro 160 acc Th r tct Se r ctg Lee att Ile a gg Arg 240 96 144 192 240 288 336 384 432 480 528 576 624 672 720 768 gtg att gca ctt gcc ctc Val Ile Ala Lee Ala Lee WO 01/00805 WO 0100805PCT/tBOO/00926 245 250 255 ggt ggt ctt ggc tgg att gtc tcc ggc gac cca gtg agg ttc ttg gct 816 Gly Gly Leu Gly Trp Ilie Val Ser Gly Asp Pro Val Arg Phe Leu Ala 260 265 270 gtt gtc gtt gtc gcc acc cca tgt cca ttg ctc att gca gtq cca gtg 864 Val Val Val Val Ala Thr Pro Cys Pro Leu Leu Ilie Ala Val Pro Val 275 280 285 gcg atc atc ggt gcg att tct ctt gcg gct cgt cgg ggc atc atc gtg 912 Ala Ilie Ilie Gly Ala Ile Ser Leu Ala Ala Arg Arg Gly Ile Ile Val 290 295 300 aag aac cct gga atq ctg gaa aac gct tca gga gta aag aca gtg atg 960 Lys Asn Pro Gly Met Leu Glu Asn Ala Ser Gly Val Lys Thr Val Met 305 310 315 320 ttc gat aag act gga acg ctc acc tat ggc agg cca gtg att act gat 1008 Phe Asp Lys Thr Gly Thr Leu Thr Tyr Gly Arg Pro Val Ile Thr Asp 325 330 335 atc cac act gct ccc gga gtt gag gaa gat aca gtc cta gct ttg gct 1056 Ilie His Thr Ala Pro Gly Val Giu Glu Asp Thr Val Leu Ala Leu Ala 340 345 350 gct tca gta gag cgc tac tcc aga cac ccg ttg qct gac gcg att cgt 1104 Ala Ser Val Glu Arg Tyr Ser Arg His Pro Leu Ala Asp Ala Ile Arg 355 360 365 gag ggc gca aaa gcc agg gaa ctt cat ctg cct gat gta gtg gaa gta 1152 Giu Gly Ala Lys Ala Arg Glu Leu His Leu Pro Asp Val Val Glu Val 370 375 380 tcg gaa cgt cca gga cag gga cta acc ggc acg gtg ggc gag cac ctg 1200 Ser Glu Arg Pro Gly Gin Gly Leu Thr Gly Thr Val Gly Glu His Leu 385 390 395 400 gtt cga ata acc aat agg cgc agc aca cta gaa att gat cca gac agc 1248 Val Arg Ilie Thr Asn Arg Arg Ser Thr Leu Glu Ile Asp Pro Asp Ser 405 410 415 aag aac tac att ccg gtg aca agt tcc ggc atg gaa tct gtg gtg ctt 1296 Lys Asn Tyr Ilie Pro Val Thr Ser Ser Gly Met Giu Ser Val Val Leu 420 425 430 gtt gat gat aaa tat gca gca ctc att cgc ctc cgg gat gaa cct cgt 1344 Val Asp Asp Lys Tyr Ala Ala Leu Ile Arg Leu Arg Asp Glu Pro Arg 435 440 445 gca tct gcc agt gag ttc atc gcg cac ttg ccc aag aag cac aaa gtg 1392 Ala Ser Ala Ser Glu Phe Ile Ala His Leu Pro Lys Lys His Lys Val 450 455 460 gac aag ctc atg att atc tct ggt gat cgc gca tct gag gtt cgt tac 1440 Asp Lys Leu Met Ilie Ile Ser Gly Asp Arg Ala Ser Glu Val Arg Tyr 465 470 475 480 ctt gcg gac aag gtt ggc att gat gag gta cac gca gag gcc tca ccg 1488 Leu Ala Asp Lys Val Giy Ilie Asp Glu Val His Ala Glu Ala Ser Pro 485 490 495 WO 01/00805 WO 0100805PCT/IB00/00926 gaa Glu atg Met acc Thr g ca Ala 545 ctc Le u ggt Gly ttc Phe ct 9 Leu gac Asp ttc Ph e gtt ValI 530 qa t Asp cac His gg C Gly ttg Leu qc t Al a 610 aag Lys tta Leu 515 ggt Gly 9 Ct Ala atc Ile a tg Met acg Thr 595 atc Ile ctg Leu 500 ggt Gly g tC Val1 9 tg Val1 ag t Ser gcq Ala 580 cca Pro ctc Leu aac Asn g at Asp gcg Al a att Ile gca Ala 565 t tg Leu Ctg Leu aat As n att Ile gga Gly atg Met tt g Leu 550 cgg Arc; agt Ser atg Met t cc Ser cgg Arc; 505 gat Asp gac Asp t cc S er cg a Arq gga Gly 585 atc Ile gtc Val1 cat His gcg Al a tcc Ser Ct 9 Le u ata Ile 570 a tg Met ttc Ph e gca Al a aat Asn cca Pro gat Asp gaa Glu 555 gcc; Ala atc Ile caa Gin ctg gag Glu gCC Ala 9 tc Val1 540 cgt Arg ttg Leu ctc Leu gag G lu cca cac His a tg Met 525 acg Th r Cc Leu caa Gln g cg Ala gtc Val1 605 cg C gCC acc Ala Thr gtt gCC Val Ala gaa gca Glu Ala gat ctg Asp Leu 560 gcg ggc Ala Gly 575 ttt gga Phe Gly gac gtg Asp Val gcg att Ala Ile 1536 1584 1632 1680 1728 1776 1824 1872 1922 1925 Leu Pro Arg 620 agt gat ttt gat acg caa gaa aaa gtt tct Ser Asp Phe Asp Thr Gin Glu Lys Val Ser 625 630 cgt <210> 580 <211> 634 <212> PRT <213> Corynebacterium glutamicum <400> 580 Lys Thr Tyr Thr Pro Asn Pro Trp Met Leu 1 5 10 Gly Ile Ile Thr Val Ala Ala Leu Val Ala 25 Leu Trp Leu Ala Leu Asp Leu Asp Gly Leu 40 Ile Ala Ile Val Ile Val Gly Gly Ile Pro 55 Lys Ser Ala Ile Lys Thr Arc; Gly Gly Ala 70 tagcagggta acctaaatgt Phe Ile Arc; Ser Phe Asp Ile Ala Ile His Leu Ile Ala Lys Asn Trp Pro Leu Leu Met Trp Asp Val Leu Asp Thr Leu Ala Ala Val 75 WO 01/00805 WO 0100805PCT[IBOOIOO926 S er Ile Arg Thr Ilie 145 His Met Lys Thr Val 225 Met Gly Val Al a Lys 305 Ph e Ile Al a Glu Ser 385 Val1 Ile Val Arg Ala 130 Ala Glu Asp Gly Ile 210 Gly Ala Gly Val1 Ile 290 Asn Asp His Ser Gly 370 Glu Arg Ile Thr Leu Met 100 Ala Ser 115 His Arg Val Glu Leu Cys Glu Ser 180 Ser Gin 195 Val Ala Val Leu Asp Arg Leu Gly 260 Val Val 275 Ile Gly Pro Gly Lys Thr Thr Ala 340 Val Glu 355 Ala Lys Arg Pro Ile Thr Ser Leu Gly Le u Glu Pro 165 Tyr Ala Thr His Le u 245 Trp Ala Ala Met Gly 325 Pro Arg Ala GI y Asn Val1 Ser Thr Leu Ile 150 Val1 Leu Met Lys Gl u 230 Gly Ile Thr Ile Leu 310 Thr Gly Tyr Arg Gin 390 Arg ILeu Gly Leu Gly 135 Thr Asp Thr Ser Leu 215 Ala Ala Val1 Pro S er 295 Glu Leu Val1 Ser Glu 375 Gly Arg Le u Gly Asp 120 Al a Val Gly Gly Gly 200 Ala Glu Trp Ser Cys 280 Leu Asn Thr Glu Ar g 360 Leu Le u Ser Gly Giu 90 Glu Ala 105 Ala Leu Thr Ile Gly Asp Glu Ile 170 Glu Pro 185 Ala Val His Asp Asn Asn Tyr Thr 250 Gly Asp 265 Pro Leu Ala Ala Ala Ser Tyr Gly 330 Giu Asp 345 His Pro His Leu Thr Gly Thr Leu Trp Leu Ala L eu Le u 155 Val Tyr Asn Ser Arg 235 ValI Pro L eu Arq Gi y 315 Arg Thr Leu Pro Thr 395 Glu Leu Val Glu Glu Arg Arg 125 Asp Gly 140 Val Ala Ala Gly Val Val Gly Asp 205 Arg Tyr 220 Pro Glu Ile Ala Val Arq Ile Ala 285 Arg Gly 300 Val Lys Pro Val Val Leu Ala Asp 365 Asp Val 380 Val Gly Ile Asp Ala Ala 110 Ala Th r Val1 His Ser 190 Thr Ala Met Leu Phe 270 ValI Ile Thr Ile Al a 350 Al a Val1 Glu Pro Ala Ilie Ala Ser Pro Ser Glu Glu Leu Pro 160 Gly Thr 175 Lys Ser Pro Leu Gin Ile Arg Arg 240 Ala Leu 255 Leu Ala Pro Val Ile Val Val Met 320 Thr Asp 335 Leu Ala Ile Arq Glu Val His Leu 400 Asp Ser wo 01/00805 WO 0100805PCT[IBOO/00926 405 410 415 Lys Asn Tyr Ile Pro Val Thr Ser Ser Gly Met Glu Ser Val Val Leu 420 425 430 Val Asp Asp Lys Tyr Ala Ala Leu Ile Arg Leu Arq Asp Glu Pro Arq 435 440 445 Ala Ser Ala Ser Glu Phe Ilie Ala His Leu Pro Lys Lys His Lys Val 450 455 460 Asp Lys Leu Met Ile Ilie Ser Gly Asp Arg Ala Ser Giu Val Argj Tyr 465 470 475 480 Leu Ala Asp Lys Val Gly Ile -Asp Glu Val His Ala Giu Ala Ser Pro 485 490 495 Giu Asp Lys Leu Asn Ile Val Asn Arg His Asn Glu His Gly Ala Thr 500 505 510 Met Phe Leu Gly Asp Gly Ilie Asn Asp Ala Pro Ala Met Ala Val Ala 515 520 525 Thr Val Gly Val Ala Met Gly Ala Asp Ser Asp Val Thr Ser Glu Ala 530 535 540 Ala Asp Ala Val Ile Leu Asp Ser Ser Leu Glu Arg Leu Asp Asp Leu 545 550 555 560 Leu His Ilie Ser Ala Arg Met Arg Arq Ile Ala Leu Gin Ser Ala Gly 565 570 575 Gly Gly Met Ala Leu Ser Val Ilie Gly Met Ilie Leu Ala Val Phe Gly 580 585 590 Phe Leu Thr Pro Leu Met Gly Ala Ile Phe Gin Giu Val Ilie Asp Val 595 600 605 Leu Ala Ile Leu Asn Ser Ala Arg Val Ala Leu Pro Arq Gly Ala Ile 610 615 620 Ser Asp Phe Asp Thr Gin Giu Lys Val Ser 625 630 <210> 581 <211> 1917 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1894) <223> RXN00980 <400> 581 aqagagaaag ggagaaatca tgaaaacgtg gaagacctgg qgggtcgtcq qagcttcagg cctcttgatt attttgtcgt ggttgagttc atcgagcccq atg ctg gca gat gca 115 Met Leu Ala Asp Ala I1 WO 01/00805 WO 0100805PCT1iBOOIOO926 ttc Phe 9 ca Ala gtt Vai geg Ala aca Thr eca Pro gag Glu ga a Giu gat Asp gge Gly 150 gtc Val eac His tta Leu g ca Ala tta Leu 230 a tg Met tat Tyr gtg Val1 geg Ala a tg Met gaa Giu ctg Leu caa Gin ga a Giu 135 tct Ser gag Glu cgc Arg gag Giu gt g Val1 215 gtg ValI ate Ile caa Gin get Ala g tg ValI aae Asn act Thr tgg T rp att Ile 120 tee Ser gat Asp gea Ala gtt Val1 aaa Lys 200 gtg Val ate Ile gcg Ala gca Ala gce Al a acg Th r eg c Arq g ea Ala gag Glu 105 ec Pro aac Asn gtg Val aca Th r gaa Gi u 185 ttC Phe gga Gi y ggc Gly get Ala ctt Leu gtt Val1 t te Ph e aca Thr aca Thr ctt Leu gtc Val1 atc Ile tat T yr gga Gly 170 gac Asp tet Ser ct t Leu t gc Cys gea Ala cgc Arg gg t Gly etc Le u eg a Arg 75 a gg Arg gag Glu gat Asp a cg Th r gea Al a 155 att Ile gee Ala aag Lys ate Ile ec Pro 235 att Ile att Ile gee Al a ttt Phe aaa Ly s ete Leu eee Pro gg a Gly ggt Gly 140 gga Gly ggt Gly eag Gin tqg Trp aee Thr 220 gge Gly gt t Val1 ega Arg a tg Met gee Ala g ea Ala aae As n ggt G ly aae Asn 125 gaa Giu aee Thr tea Ser ga t Asp tae Tyr 205 tgg Trp geg Al a ggt Gly 15 gtg Val1 ate Ile gge Gly t eg Ser gat Asp 95 ate Ile att Ile a tg Met etg Leu tea Ser 175 aaa Ly s eeg Pro gta Val1 gtt Val tgg Trp t eg Ser aae As n aag Lys gat Asp 80 ga e Asp gte Val 9g Ala eeg Pro ega Arg 160 aet Thr geec Ala gge Gly ga a Giu ate Ile 240 e agc Gin etg Leu g agc Giu eg e Arg gea Ala gta Val1 100 aat Asn gg a Gly ggCit Gly ttg Leu ate Ile 180 aca Thr gee Ala a eg Thr tet Ser gte Val tet Ser g eqg Ala get Ala gtt Val1 Gly att Ile eaa Gin a ga Arg 165 att Ile tte Phe gee Ala ete Le u 163 211 259 307 355 403 451 499 547 595 643 691 739 787 835 tee ate eeg gtg tee Ser Ile Pro Val Ser 245 WO 01/00805 WO 0100805PCT/IBOO/00926 atc Ile ggt Gly ga c Asp ga a Glu gcg Ala 310 cg t Arg ga a G lu gt t Val1 a gg Arg qtg Val 390 t ca Se r gtt Val1 tC Ser aaa Lys a tg Met 470 atc gtc Val gga Gly aaa Lys gtC ValI 295 cgc Arg ggt Gly aac Asn gct Ala atc Ile 375 aac Asn ga t Asp gtc Val1 aag Ly s tta Leu 455 gtg Val1 gga gca Ala gaa Glu act Th r 280 atc Ile gcc Al a 9CC Ala atc Ile gt t Val1 360 ctg Leu gga Gly tct Ser atg Met ctg Leu 440 gaa Glu gg t Gl y gta ggc atc Gly Ile 250 tac cta Tyr Leu 265 gga acg Gly Thr gag ccc Glu Pro gag acg Glu Thr cag gat Gin Asp 330 acc ggc Thr Gly 345 gga tct Gly Ser gag cta Glu Leu cac gcc His Ala gcc tca Ala Ser 410 gcg act Ala Thr 425 gga gtg Gly Val ctg gtg Lev Val gac gga Asp Gly gcg atg ggc cgt gct gca cgc Gly Arg Ala Ala gat ggc gtg Asp Gly Val ctg atc aag Leu Ile Lys gaa Glu Ct 9 Leu C cc Al a gct Ala 315 cg99 Arg cqa Arg gqt Al a aat Asn att I le 395 gca Ala 99 C Gl y gat Asp cg t Arg gtC Val1 475 99C acc Thr a cc Thr tac T yr 300 t ca Ser 999 Gly ggC Gly gag Glu 9CC Ala 380 gga Gly atc Ile gac Asp gaa Glu ga t Asp 460 aac As n 9 tg 9CC Al a acc Thr 285 agc Se r gaa G lu ctg Le u att Ile tta Leu 365 ga a Glu atc Ile ga a Glu gct Ala gt C Val1 445 ctg Leu gac Asp gca gcg Ala 270 ggC Gly cag Gin cat His t CC Se r atc Ile 350 ctt Leu gga Gly gtg ValI t Cq Ser cac His 430 tac Tyr ca a G in acc Thr 99 t Arg 255 aaa Lys cgc Arg ggC Gly ccg Pro aca Thr 335 9 ca Ala gat Asp aag Ly s

C

Al a Ct 9 Leu 415 cgc Arg tca Ser gct Al a cca Pro tcc 260 9 tC ValI cca Pro gag Glu ctt Leu 320 a ca Th r aat As n cat His acc Thr gtc Val1 400 cat His gtt Val1 gag Glu 9CC Ala gca Ala 480 cct ga c Asp gaa Glu gtg Val1 305

C

Ala tt 9 Leu gt t Val gaa Glu gcg Ala 385 9CC Ala a ag Lys gca Ala cta Leu 99 C Gly 465 ttg Leu 9 ca gtc Val1 ct C Leu 290 Ctg Leu ga c Asp gtg Val ga t Asp cca Pro 370 atg Met gac Asp g9 Ala caa Gin ctc Leu 450 aaa Lys gca Ala gCC gt t Val1 275 aca Thr gag Glu 9CC Ala gaa Gi u gga Gly 355 gac Asp ttt Phe 9CC Ala ggC Gly aac Asn 435 cct Pro a cg Thr gc t Ala att 9 tC Val1 gac Asp ctc Leu atc Ile gca Ala 340 cag Gin t cg Ser gtc Val1 gtt Va 1 att Ile 420 gt 9 ValI gaa Gi u gtc ValI gct Ala gaa gtg Val1 qta Val1 9CC Ala atc Ile 325 gct Ala gca Al a acc Th r 99 a Gly cg t Arg 405 caa Gin 9CC Ala cag Gin 9 C9 Ala ga t Asp 485 acc 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 WO 01/00805 Gly Val Ala Met Gly Vai Ala 490 gat atc gca ctc atg gcg gat Asp Ile Ala Leu Met Ala Asp 505 acc ttg gca aaa cgc acc gta Thr Leu Ala Lys Arg Thr Val 520 525 gcg ttg gct acc gtg atg gtg Ala Leu Ala Thr Val Met Val 535 540 gtt acc atg tcg gtt ggc atg Val Thr Met Ser Val Gly Met 555 gtt atc agc atc gcc atq ctg Val Ile Ser Ile Ala Met Leu 570 gct gcg caa gca agt gat att Ala Ala Gin Ala Ser Asp Ile 585 taaccaatgg ctgggtactg atg PCT/1BOO/00926 Ala Cgg Arg cgc Arg gg C Gly 545 ga a G iu cca Pro gaa G iu 1651 1699 1747 1795 1843 1891 1917 <210> 582 <211> 598 <212> PRT <213> Corynebacterium giutamicum <400> 582 Met Leu Ala Asp Ala Phe Met Ile Ala 1 Pro Ilie Ala Gin Ser Ala Tyr Gin Ala Ilie Asp Leu Leu Val Val Val Ala Ala 40 Asn Tyr Trp Giu Ser Ala Ala Val Thr 55 Ala Leu Giu Arg Ala Thr Met Asn Arg 70 Leu Val Asp Ala Ala Pro Glu Thr Ala Ser Thr Giu Val Val Giu Leu Trp Giu 100 105 Leu Val Arg Asn Gly Giu Gin Ilie Pro 115 120 Ala Arg Gly Le u Arcj Arg Giu Asp Ile Ile Ala Ph e Lys Leu Pro Gly Val1 Arg Met Ala Ala As n Gly Asn 125 WO 01/00805 PCT/IB00/00926 Gly Val Gly Gly lie Asp Glu Ser Asn Ile Thr Gly Glu Ser Met Pro 130 135 140 Ala Glu Lys Gly Gin Gly Ser Asp Val Tyr Ala Gly Thr Trp Leu Arg 145 150 155 160 Ser Gly Val Leu Arg Val Glu Ala Thr Gly Ile Gly Ser Asp Ser Thr 165 170 175 Leu Ala Lys Ile Ile His Arg Val Glu Asp Ala Gin Asp Asp Lys Ala 180 185 190 Arg Thr Gin Thr Phe Leu Glu Lys Phe Ser Lys Trp Tyr Thr Pro Gly 195 200 205 Val Met Ile Ala Ala Ala Val Val Gly Leu Ile Thr Trp Asp Val Glu 210 215 220 Leu Ala Leu Thr Leu Leu Val Ile Gly Cys Pro Gly Ala Leu Val Ile 225 230 235 240 Ser Ile Pro Val Ser Ile Val Ala Gly Ile Gly Arg Ala Ala Arg Asp 245 250 255 Gly Val Leu Ile Lys Gly Gly Glu Tyr Leu Glu Thr Ala Ala Lys Val 260 265 270 Asp Val Val Val Val Asp Lys Thr Gly Thr Leu Thr Thr Gly Arg Pro 275 280 285 Glu Leu Thr Asp Val Glu Val Ile Glu Pro Ala Tyr Ser Gin Gly Glu 290 295 300 Val Leu Glu Leu Ala Ala Arg Ala Glu Thr Ala Ser Glu His Pro Leu 305 310 315 320 Ala Asp Ala Ile Ile Arg Gly Ala Gin Asp Arg Gly Leu Ser Thr Thr 325 330 335 Leu Val Glu Ala Ala Glu Asn lie Thr Gly Arg Gly Ile Ile Ala Asn 340 345 350 Val Asp Gly Gin Ala Val Ala Val Gly Ser Ala Glu Leu Leu Asp His 355 360 365 Glu Pro Asp Ser Thr Arg Ile Leu Glu Leu Asn Ala Glu Gly Lys Thr 370 375 380 Ala Met Phe Val Gly Val Asn Gly His Ala Ile Gly Ile Val Ala Val 385 390 395 400 Ala Asp Ala Val Arg Ser Asp Ser Ala Ser Ala Ile Glu Ser Leu His 405 410 415 Lys Ala Gly Ile Gin Val Val Met Ala Thr Gly Asp Ala His Arg Val 420 425 430 Ala Gin Asn Val Ala Ser Lys Leu Gly Val Asp Glu Val Tyr Ser Glu 435 440 445 Leu Gly 465 Leu Ala Arg Arg Gly 545 Gi u Pro Glu WO 01/00805 Leu Pro Glu 450 Lys Thr Val Ala Ala Ala Ala Ilie Giu 500 Leu Ala His 515 Ile Asn Ilie 530 Val Leu Phe Ala Ser Val Thr Leu Lys 580 Ilie Gin Gin 595 Leu 455 ValI Gly Asp Th r Ala 535 Val1 Val1 Al a Glu.

Gi y Val1 Ile Leu 520 Leu Th r Ile Ala Gin Thr Gly Arg 510 Arg Le u Le u Le u Lys 590 Ala Pro Ser 495 Leu Thr Leu Val1 Leu 575 Arg PCT/IBOO/00926 Ala Al a 480 Pro Pro Met Ala His 560 Arg Ser <210> 583 <211> 1296 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (1273) <223> RXN00099 <400> 583 ctctggtgaa gaggatgttg actcgggaga ttcttccact ccgcgcaaat aggtagtcgc ttgcttatag ggtcaggggc gc t Ala gct Ala ct t Leu g cg atc Ile ctg Le u gg t Gly gcg tcg Ser gag Glu 9qC Gi y tcg gattcactga ttaagtggta gtg aag aat cct cgc 115 Val Lys Asn Pro Arq 1 ttc aat ctg cga aca 163 Phe Asn Leu Arg Thr att cgg gat gat tta 211 Ilie Arg Asp Asp Leu atg atc ccg act gct 259 Met Ile Pro Thr Ala ttg aag agg aag ttc 307 Met act Thr cag Gin gtg Val1 gct Ala tat Tyr gtq Val1 150 gtg Val1 tgg T rp t cg Ser 9gg Gly ttt Ph e 230 ggC Gly ttg Leu gtc ValI gcg Ala WO 01/00805 Phe Ala Asp act tcc caa Thr Ser Gin att att cgt Ile Ile Arg ttc gcg atg Phe Ala Met 105 gtt agg gag Val Arg Glu 120 ctg gtq tcg Leu Val Ser 135 ccg att tct Pro Ile Ser tcq ctc gqt Ser Leu Gly att ccg ctg Ile Pro Leu 185 aag gtt tct Lys Val Ser 200 ttg atg ttt Leu Met Phe 215 atg ccg cag Met Pro Gin tgg tgg tca Trp Trp Ser gtg acg cgt Val Thr Arg 265 atg ttt ctc Met Phe Leu 280 ccg tgg ttg Pro Trp Leu Ala c tg Leu gtc Val ttt Phe tat T yr ttc Phe cag Gin tcg Ser 170 ttg Leu ctt Leu ggg Gly atg Met att Ile 250 ttc Ph e atc Ile t gg Trp Aia ttg Leu 75 gct Aia gcg Ala ttt Ph e ca g Gin t gq Trp 155 tgg Trp agt Ser cct Pro ttt Phe g ta Val 235 ttg Leu act Thr ggt Giy gcg Ala Ph e 60 a tg Met qqa Gly atc Ile ccg Pro att Ile 140 gct Ala gcg Aia t tq Leu gtg Va 1 act Th r 220 gg t Gly gga Gly aac Asn aat Asn a cg Thr Al a ttt Phe cc t Pro gga Gly cg t Arg 125 qt t ValI aca Th r ct g Leu ca g Gin tgg Trp 205 t cg Se r gat Asp ttg Leu tgc Cys ggt Giy 285 ttg L eu Leu gcc Ala gc t Ala gtt Val1 110 cac His cag Gin cat His ct g Leo ggt Gly 190 a ag Lys ttt Ph e cct Pro ccg Pro ttc Phe 270 ggg Gly tct Se r Pro atg Met t cg Ser 95 acc Thr g tc Val gca Ala qtg Val ggg Gly 175 gcc Ala tct Se r gg Al a ca g Gin ctg Leu 255 c cg Pro ttt Phe qg t Gly Ser ctg Leu 80 ctg Leo aat Asn ggt G ly ctt Leu ggg Gly 160 ttg Leo agg Arg t cg Ser a cg Th r ct c Leo 240 aa c As n atg Met tg t Cys ctt Leo Leo t t g Leo ttg Leu gtg Val1 gga Gly gct Ala 145 ttg Leo gtt Val gt t Vali gtt Val tat Tyr 225 gg9t Gly att Ile gtt Val1 ttg Leo ggt Gly Lys act Thr a tg Met ttg Leo atg Met 130 ccg Pro a cc Thr gcg Ala gtt Val gg t Gly 210 atc Ile gcg Al a ct g Leo gtt Val1 gct Al a 290 ccc Pro Arg gc t Ala gtc Val1 ctt Leo 115 t cg Se r acg Thr ggt Gi y gcg Ala g cg Ala 195 g tg ValI ctc Leo gtg Val1 gga Gly atc Ile 275 ccg Pro ct t Leu Lys gc c Ala ggt Gly 100 ccg Pro aca Thr ctt Leo tg Trp att Ile 180 g cg Ala ggg Gly atg Met ttg Leo ccg Pro 260 gcc Ala ga t Asp g cg Al a PCTIBOOIOO926 Phe ggt 355 Gly act 403 Th r att 451 Ile act 499 Thr qcc 547 Al a aqg 595 Arg 165 tcg 643 Ser ccg 691 Pro ctc 739 Leo qgt 787 Gly tta 835 Leo 245 tgg 883 T rp agt 931 Ser gtt 979 Val1 ttc 1027 Phe WO 01/00805 WO 0100805PCT/UBOO/00926 295 300 ccg atg gcg ttg acg ctc att aat Pro Met Ala Leu Thr Leu Ilie Asn 310 315 gct tct gcg ttg agt tcc ttc ggg Ala Ser Ala Leu Ser Ser Phe Gly 330 tgt ttc ggt ccc ttg ttg act ggtI Cys Phe Gly Pro Leu Leu Thr Gly 345 ttc cga aca atc ttt gtg ctt ttt Phe Arg Thr Ile Phe Val Leu Phe 360 365 aga ggc ggt tac ttt gcg aca agg Arg Gly Gly Tyr Phe Ala Thr Arg 375 380 aat cgc taggatggcg ctatgccgca aa Asn Arg 390 <210> 584 <211> 391 <212> PRT <213> Corynebacterium glutamicum att Ile cag 31n :tc ?he 350 9c9 kia zag Gln gct Ala att Ile 340 ggc Gly gtt Val1 ctt Leu gg t Gi y 325 gcg Ala agc Ser att Ile tta Leu 1075 1123 1171 1219 1267 1296 <400> 584 Val Lys Asn Pro 1 Phe Ilie Met Le u Leu Leu Val Gi y Ala As n Arg Ile Lys Th r Met Leu Met 130 Pro Arg Asp Th r Lys Ala Gi y 100 Pro Th r Leu Arq 5 Th r Leu Ala Phe Gly Thr Ilie Thr Ala Leu Ile Ala Leu Ala Ala Ilie Ilie Leu Thr Ser Ala Gi y Met Th r 70 Gin Val Ala Tyr Val1 Ile Val Phe 55 Th r Ile Phe Val1 Leu 135 Pro Thr Ser Ala Ser Ile Ala Arg 120 ValI Ile Ala Ala Asp Gin Arg Met 105 Giu Se r Ser 10 Le u Ser Ala Leu Val1 90 Phe Tyr Phe Gin Ala Le u Ala Leu 75 Ala Ala Ph e Gin Trp Pro Ile Ph e Met Gly Ile Pro Ile 140 Ala Leu Gly Ala Phe Pro Gly Arg 125 Val1 Thr Ser Le u Pro Met Ser Thr Val1 Ala Val1 Glu Gly Ser Leu Leu Asn Giy Leu Gly WO 01/00805 145 Leu Val Val1 Val1 Tyr 225 Gly Ile Val1 Le u Gly 30.5 Glu Gly Asp Thr Val1 385 155 Ser Leu Gly Ser Trp Ala Leu Leu Thr Ala Val Gly 210 Ile Al a Leu Val1 Ala 290 Pro Thr Tyr Ala Le u 370 Glu Gly Ala Al a 195 Val Le u Val1 Gl y Ile 275 Pro Le u Ser Th r Th r 355 Phe Ly s Trp Ilie 180 Ala Ci y Met Le u Pro 260 Ala Asp Al a Ala Ile 340 Gly ValI Le u Arg 165 Ser Pro Leu Gly Leu 245 T rp Se r ValI Phe Gly 325 Ala Ser Ile Leu Val1 Trp Se r Gly Phe 230 Gly Le u Val1 Ala Pro 310 Ala Cys Phe Arg Asn 390 Ile Lys Leu 215 Met T rp Val1 Met Pro 295 Met Ser Phe Arg Gly 375 Arg Pro ValI 200 Met Pro Trp Thr Phe 280 Trp Al a Ala Cl y Thr 360 Cly Leu 185 Ser Phe Gin Ser Arg 265 Leu Le u Le u Le u Pro 345 Ile T yr Ser Pro Ph e Val 235 Le u Thr Gly Ala Le u 315 Ser Leu Val1 Ala Leu Val1 Thr 220 Cly Gly As n Asn Thr 300 Ile Phe Thr Leu Thr 380 Gin Trp 205 Ser Asp Le u C ys Gly 285 Le u Asn Gly Gly Ph e 365 Arg Gly 190 Lys Ph e Pro Pro Phe 270 GI y Se r Ile Gin Phe 350 Al a Gin PCTLBOO/00926 160 Gly Leu 175 Ala Arg Ser Ser Ala Thr Gln Leu 240 Leu Asn 255 Pro Met Phe Cys Cly Leu Arg Ala 320 Gly Leu 335 Ile Val Val Ala Val Tyr <210> 585 <211> 387 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (364) <223> RXN02662 <400> 585 ccaaggtgtc accctcaccg ttqccatcgc cttcatcatc gtcaatatcg ccgtggacct WO 01/00805 gctctacgtc ctggtcaatc cacgtattag gagcatctag acc Th r gca Ala ttc Phe gCC Ala acc Thr gag Glu acc Th r ctt Leu ct C Leu tcC Se r ga C Asp cct Pro tta gaa aac aag ccc ggt qca Leu Giu Asn Lys Pro Gly Ala cca act tca aaa atc gcg ctg Pro Thr Ser Lys Ile Ala Leu 30 gcg atc ttt gcc cca ctg att Ala Ilie Phe Ala Pro Leu Ile act cca gtc cag cct cca agc Thr Pro Val Gin Pro Pro Ser atc gqc cgc gat att ttc tcc Ile Gly Arg Asp Ile Phe Ser 75 tgatcattgg tcttttcgct acg Cg t Arg ggc Gly Ctc Le u gat Asp tgg Trp acg Thr PCTIBOOIOO926 cta 115 Leu cgc 163 Arg atc 211 Ile ctg 259 Leu ggt 307 Gly cca 355 Pro <210> 586 <211> 88 <212> PRT <213> Corynebacterium qiutamicum <400> 586 Met Arg Arg Lys Leu Thr Thr Thr Leu Glu 1 5 10 Leu Giy Gly Phe Arg Ala Leu Ala Pro Thr 25 Phe Leu Leu Leu Ile Phe Leu Leu Ala Ile 40 Lys Tyr Asp Pro Leu Ala Ser Gly Thr Pro 55 Giu His Trp Phe Gly Thr Asp Ala Ile Gly 70 Val Ala Thr Ala Pro Glu Pro Pro <210> 587 <211> 972 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS Lys Pro Gly Ala Arg Lys Ile Ala Leu Val Ala Pro Leu Ile Ala Gin Pro Pro Ser Gly Asp Ile Phe Ser Arg WO 01/00805 <222> (949) <223> RXN02442 <400> 587 gccgtgatqt tgttgagcgc gatgtgattg ccgtatgtgc agttctgcca ttccttaatg ataacggtta tcattttcaa PCTIBOOOO926 atgtgagatt ccggacgctg gac Asp gtc Val1 gtt Val1 ttg Leu ttc Phe acc Th r ttt Ph e tcc Se r gt g Val gga Gly 150 ga c Asp ct a Le u gtg Val g cc Ala gcc Ala att Ileh ct C Leu ggc Gly aqc Se r att Ile cac His cga Arg 135 t tg Leu gag Glu ctc Le u gtg Val1 ctc Leu gga Gly ttg Leu ccc Pro gca Ala aga Arg acc Thr gcc Al a 120 ccc Pro act Thr cgt Arg atg Met gga Gly 200 cct Pro gcc Ala cta Le u g cc Ala agc Ser 75 aqc Se r tcC Ser ctc Leu ata Ile ctc Leu 155 cac His ct g Leu tta Leu ttt Phe g ca Ala acc Thr acg Thr 60 g ca Ala ctc Le u ctc Le u a cc Thr ttc Phe 140 ttc Phe acc Th r atc Ile gtg Val gac Asp att Ile ttt Phe 45 gca Ala tta Leu tcc Ser ggc Gly agt Ser 125 atc Ile cac His tta Le u g ca Al a ttt Phe 205 gtt Val1 tta Leu ttc Phe t ca Ser atc Ile caa Gin gt g Val1 110 ttc Phe atc Ile cg a Arg gq a Gi y tta Leu 190 gga Gi y ttc Phe t ca Se r gac Asp ttg Le u tca Ser 80 gt c Val1 att Ile ttt Phe att Ile ttc Phe 160 aat Asn a cg Th r ctc Leu atg Met 1 atc Ile ctc Leu gct Ala 99c Gly gcc Ala agc Ser gtg ValI gga Gly g ca Ala 145 act Thr ccc Pro gtg Val1 att Ile t cc Se r att Ile atg Met gga Gly gga Gly att Ile tcc Ser gac Asp 130 aca Thr gca Ala cgc Arg gt q Val1 ggt Gly 210 cgc Arg gga Gly t cq Ser aat Asn gtg Val1 ggc Gly cac His 115 att Ile gtg Val1 ctc IA u ttt Phe t cg Se r 195 ccg Pro aag ttt ttt act Lys Phe Phe Thr gcc Ala a cg Th r cac His ctc Le u gtg ValI ctg Le u 100 tcc Se r ct t Leu ttg Leu gct Ala g ca Ala 180 ttt Phe ccc Pro c tg Le u t gg Trp gci Gly atg Met t gg Trp ca a Gin ga t Asp 9gc Gly ggt Gly ttc Phe 165 cac His ca 9 Gin gcc Ala 115 163 211 259 307 355 403 451 499 547 595 643 691 739 WO 01/00805 PCTABOOIOO926 787 qca Ala gcg Ala t gg Trp 9c9 Ala aac As n 280 ctt Le u t cg Se r cac His ata Ile 265 ttc Phe tta Leu ctt Le u gca Ala 250 ttt Phe acc Thr caa gac aaa gca agt att tca Gin Asp Lys Ala Ser Ile Ser 220 225 gga tgc qcg qaa att tac ctc Gly Cys Ala Giu Ile Tyr Leu 240 act gcc gcq qga gcc act atc Thr Ala Ala Gly Ala Thr Ile 255 gcacc tta ttg aca aag agt Ala Thr Leu Leu Thr Lys Ser 270 tgatactgaa agacattttc aat <210> 588 <211> 283 <212> PRT <213> Corynebacterium qiutamicum <400> 588 Met Lys Phe Phe Thr Asp Ala Leu Ile Val Pro Phe Asp Val Ser Phe 1 Ile Le u Ala Gly Ala Ser ValI Gly Ala 145 Thr Se r Ile Met Gly Gi y Ilie Ser Asp 130 Th r Ala Arq Gi y Se r As n Val1 Gly His 115 Ile Val Leu Ala Thr His Le u ValI Le u 100 Se r Le u Leu Ala 5 Le u Trp Gly Met Trp Gin Asp Gi y Gly Phe 165 Val1 Val1 Le u Phe 70 Thr Phe Ser Val G ly 150 Asp Ala Ile Leu 55 Gly Ser Ile His Arg 135 Le u Glu Gly Le u 40 Pro Ala Arq Th r Ala 120 Pro Th r Arg Cys 25 Arg Gly Ala Ly s Met 105 Val1 Ser Ile Lys 10 Leu Arg ValI Ile Se r 90 Le u Asp Asp Ph e Ala 170 Ala Le u Ala Ser 75 Se r Ser Le u Ile Le u 155 Ala Th r Thr Ala Le u Le u Thr Phe 140 Phe Ile Phe Ala Le u Se r Gly Ser 125 Ile His Le u Phe Ser Ile Gin Val 110 Phe Ile Arg Cys Gly Leu Met Asp ValI Le u Ala Gin Se r Asp Le u Se r Val1 Ile Phe Ile Phe His Thr Leu Gly Leu Asn 175 Pro Val Ile Ile 225 Tyr Th r Ly s WO 01/00805 Arg Phe Al 18 Val Ser Ph 195 Gly Pro Pr 210 Ser Leu Ii Leu Gly Le Ilie Thr Le 26 Ser Ala Ii 275 PCT/IBOO/00926 His Gin Ala Met Le u 245 Leu Ser Ile Val Gin 220 Giy Thr Ala Ala Phe 205 Asp Cys Ala Thr <210> 589 <211> 1077 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1054) <223> RXN02443 <400> 589 caagcactgc cgcgggagcc actatcactt tgttaagtgc tattgacaaa gagtgccatt agtaggttaa acttcaccgc att Ile cg a Arg atc Ile acc Th r ga t Asp gtc Val1 ttc Phe aaa Lys acc Thr aac Asn gtC Val tca Se r aat Asn cta Leu gcc Al a atc Ile caa Gin gc a Ala aa t As n cta Le u tgt Cys ctg Leu gta ValI caa Gin ggg Gi y gc t Ala tcc Ser 99 t Gly ctc Le u ga c Asp ttt Phe gCC Ala ga t Asp 45 gta ValI

CCC

Pro 9CC Al a 999 Gi y gt t Val1 30 ga C Asp a qc Se r aac As n atg Met t CC Se r gcc Ala CC t Pro atc Ile ga t Asp 80 cat His tqcgatattt tttqccacct gtg ata ctg aaa gac Vai Ile Leu Lys Asp 1 tcc gcg aaa aat ttc Ser Ala Lys Asn Phe tca cta gct ttt ggt Ser Leu Ala Phe Gly gac att gtg gtc acc Asp Ile Val Val Thr gtg gga gat tcc gca Val Gly Asp Ser Ala cca cat tcc ttc gga Pro His Ser Phe Gly 9CC gat ctc atc gtg Ala Asp Leu Ile Val 115 163 211 259 307 355 403 WO 01/00805 WO 0100805PCT/IBOO/00926 gcc Ala gcc Ala gtc Val1 c cg Pro 150 aaa Ly s cac His ctc Leu gt t Val aic Ilie 230 aat As n acc Thr gct Ala gat Asp gcc Ala 310 taa aat As n aaa Lys att Ile 135 gcg Ala gaa Giu tac Tyr agc Se r ttt Phe 215 atc Ile ga c Asp gat Asp 9gC Gi y gca Ala 295 gga Gly agc Ser 120 gac Asp cgc Arg ctc Leu cg i Arg ggc Gly 200 gga Gly c ca Pro atc Ile acc Thr att Ile 280 gat Asp cia Le u 105 caa Gin tac Tyr atg Met gat Asp gag Giu 185 gtg ValI tac Tyr gg t Gly icc Ser i ca Se r 265 ga t Asp ggt Gly gga Gly gg g Giy icc Se r aic Ile cci Pro 170 ga a Giu g cc Ala ctq Leu gga Gly acc Thr 250 agc Ser gt Val gaa Glu ct Leu gt Vai ccc Pro gcc Ala 155 icc Ser cii Le u cca Pro qcc Ala agc Se r 235 gcc Ala cca Pro ca a Gin gca Ala gaa Giu ccc Pro ggc Gly 140 gc Ala cig Le u gt Val1 gaa Giu agc Ser 220 aca Th r aic Ile caa Gin gig Val1 ccc Pro 300 gag Glu gi c Val1 125 gt Val acg Thr act Thr gcc Ala aac As n 205 cgg Arg tig Leu gaa Giu Arg gig Val 285 acc Thr ggc Gi y 110 i ig Le u cca Pro ga a Giu gaa Glu cii Leu 190 cg c Arg ttt Phe gcg Ala ga c Asp tia Le u 270 icc Ser tac T yr 95 ct Leu gaa Giu gat Asp gt Val1 i cg Se r 175 gat Asp aa g Lys aac Asn g cg Ala aac Asn 255 gci Ala ati Ile aic caa Gin gic Val cci Pro ata Ile 160 aic Ile gag Giu cig Leu tac Tyr cct Pro 240 aai Asn gaa Giu tc Phe ag c icc Se r gg9c Gly ca c His 145 gaa Giu aca Thr ga a Giu gia ValI acc Thr 225 i ca Se r gt Val gtg Val1 acg Th r aig aai As n gaa Giu 130 ttt Phe gci Ala caa Gin gtc Val1 acc Thr 210 gic Val1 gca Ala ccc Pro tig Leu gaa Giu 290 caa gig Val1 115 cac His t gg T rp gaa Giu ica Ser acc T hr 195 aat Asn ati Ile ict Se r gca Ala gcc Ala 275 tca Se r aaa 100 gac aai Asp Asn aic gat Ile Asp aca gac Thr Asp cig aic Leu Ile 165 gcc cag Ala Gin 180 gaa tig Giu Leu cac aai His Asn gac acc Asp Thr gac cic Asp Leu 245 atc tic Ile Phe 260 agc aac Ser Asn cic acc Leu Thr aic aat 451 499 547 595 643 691 739 787 835 883 931 979 1027 1074 1077 Ile Ser Met Gin Lys Ile Asn 305 gag cgc ait gca Giu Arg Ile Ala act tig icc Thr Leu Ser iaaacagtcc taaacagtci WO 01/00805 <210> 590 <211> 318 <212> PRT <213> Corynebacterium glutamicum PCTIBOO0926 <400> 590 Val Ile Leu Lys 1 Ser Ser Asp Val Pro Ala Ser Gly His 145 Glu Thr Giu Val1 Thr 225 Se r ValI Val1 Ala Le u Ile Gly His Asp Asn Glu 130 Phe Ala Gin Val1 Thr 210 Val1 Ala Pro Le u Lys Ala ValI Asp Se r Le u ValI 115 His T rp Giu Ser Thr 195 Asn Ile Ser Ala Ala 275 As n Phe Val1 Se r Phe Ilie 100 Asp Ile Th r Leu Ala 180 Giu His Asp Asp Ile 260 Se r Asp 5 Phe GI y Thr Ala Gly Val As n Asp Asp Ile 165 Gin Le u Asn Thr Leu 245 Phe Asn Ile Phe Arg Ile Thr Asp 70 Val Ala Ala Val1 Pro 150 Lys His Leu Val1 Ile 230 Asn Thr Ala Ly s Th r As n 55 Val1 Se r As n Lys Ile 135 Ala Giu Tyr Ser Phe 215 Ile Asp Asp Gly Asn Asn Gly 10 Leu Leu Ala 25 Ala Cys Ser 40 Ile Leu Giy Gin Val Leu Ala Gin Asp 90 Gly Leu Gly 105 Ser Gin Gly 120 Asp Tyr Ser Arg Met Ile Leu Asp Pro 170 Arg Giu Glu 185 Gly Val Ala 200 Gly Tyr Leu Pro Gly Gly Ile Ser Thr 250 Thr Ser Ser 265 Ile Asp Val 280 Asp Gly Glu Giu Val1 Ala Asp Met 75 Ala Leu Val1 Pro Ala 155 Ser Leu Pro Ala Ser 235 Ala Pro Gin Ala Leu Pro Val1 Val1 Lys Ala Giu Pro Gly 140 Ala Leu Val1 Giu Ser 220 Thr Ile Gin Val1 Pro Phe Ala Asp ValI Pro Ala Giu Val 125 Val Thr Thr Ala Asn 205 Arq Leu Giu Arg Val1 285 Thr Gly Val1 Asp Se r As n Met Gly 110 Leu Pro Giu Giu Leu 190 Arg Phe Ala Asp Leu 270 Se r Tyr Al a Ala Thr His Ala Giu Le u Giu Asp ValI Ser 175 Asp Lys Asn Ala Asn 255 Al a Ile Ile Ser Al a Pro Ile Asp His Gin Vali Pro Ile 160 Ile Giu Le u T yr Pro 240 Asn Giu Phe Se r Thr Giu Ser Leu Thr Asp Ala WO 01/00805 290 295 300 Met Gin Lys Ile Asn Ala Giu Arg Ile Ala Ser Thr Leu Ser 305 310 315 <210> 591 <211> 1323 <212> DNA <213> Corynebacterium glutarnicum <220> <221> CDS <222> (1300) <223> RXN00842 <400> 591 ccttgtcgcg gaggtaagcg agggtatttc tggatgtgga acaacgcgga PCT11BOO/00926 ttatggaaaa tcqtgacttt cataacgttg aqcctactaa ggtttgttcc att att caa atc Ile Ile Gin Ile cta Leu gca Ala gga Giy ttg Leu gaa Giu atc Ile Gly 99 t Giy gtg Vali att Ile aga gtg Arg Vai tca ggg Ser Giy att tgg Ile Trp atc att Ile Ilie gtg ttc Val Phe ggc gtt Gly Val gcg gga Aia Giy tct gtg Ser Vai 120 gat aaa Asp Lys 135 ttc atc Phe Ile g ca Aila caa Gin gga Giy t tq Le u cgc Arg gtg Vali tca Ser 105 atc Ile gt a Vali act Th r ttt Ph e gag Giu gtc ValI cag Gin cga Arg gtg Vali aat Asn atg Met ac Thr gcc Ala qcc Aia gt c ValI att Ile ctc Leu gta Vali 75 a cg Thr ctg Le u gtt Vali aca Thr gcc Ala ttc Phe atg Met gtt ValI gga Giy 60 agt Ser ttg Le u aat Asn ctt Le u gtc Val 140 tcg Se r gtc Val1 ca a Gin tct Ser 45 agc Ser ca c His ttc Phe ca g Gin ctq Leu 125 att Ile atc Ile ggc Giy tat Tyr 30 g ca Ala tat T yr ccc Pro tcc Ser ca g Gin 110 gtg Val1 gg t Gly tac Tyr atc Ile 15 ttt Phe gtg ValI ttc Ph e gt t Va 1 acc Thr 95 tgg Trp ctg Le u gca Al a a cg Thr 1 att gtt ggc Ile Val Gly gtg gcc ttc Val Ala Phe atc atg tcg Ile met Ser aat gca ggt Asn Ala Gly ttc tcc aaa Phe Ser Lys 80 ggt ttc gtc Gly Phe Val ggq ctt ccg Gly Leu Pro qct gcg ggc Ala Ala Gly 130 att act ccg Ile Thr Pro 145 ctg gta ggt Leu Val Gly gcc Al a 9gc Gly gt g Val gaa Glu att Ile atg Met ctc Le u 115 atg Met ttc Phe 99 t Gly ata Ile atg Met cac His ttg Leu ttt Phe 100 tg Trp ttg Le u atc Ile ttc Phe ga C Asp gcg Ala ggt Gly ga C Asp gca Ala atc Ile ga c Asp atc Ile 115 163 211 259 307 355 403 451 499 547 595 aat ttc agc Asn Phe Ser WO 01/00805 150 t ca Ser cct Pro gt Val Cgg Arg t ig Le u 230 ggc Gly ctg Leu a cg Thr aac Asn iii Phe 310 cca Pro g cg Ala atg Met gga Gly gig Val1 cac His gcg Ala gt c Val1 215 atc Ile caa Gin gct Aila gca Ala cca Pro 295 gtg Val gt c Vai igg Trp cgg Arg gca Ala 375 gag Giu tgg Trp gig Val1 200 gca Ala atc Ile ga t Asp ggc Giy ci g Leu 280 ca g Gin itg Leu iig Leu gig Val1 atc Ile 360 gag Glu cag Gin gci Ala 185 icC Ser ggt Gly atc Ile gati Asp caa Gin 265 ggi Giy cga Arg agc Se r gga Giy agg Arg 345 gca Al a ctg Leu 155 cii gai Leu Asp 170 gii gca Vai Ala aig gci Met Ala cgg ggc Arg Gly agt gcg Ser Ala 235 aig cct Mei Pro 250 gia aig Vai Mei aig itc Met Phe tic cgi Phe Arg itt gig Phe Val 315 iac aii Tyr Ile 330 gga cgc Giy Arg gac iig Asp Leu gcg gig Ala Val ici gct gci tia Ser Ala Ala Leu 175 gcg gig aac tai Ala Val Asn Tyr 190 gig gic ati ggt Val Val Ile Gly 205 ggt tig cig ggc Gly Leu Leu Gly 220 cia aca ctg ttc Leu Thr Leu Phe atg ctg acg atc Met Leu Thr Ile 255 gca gig gtt aic Ala Val ValIle 270 tac gca tig ggc Tyr Ala Leu Giy 285 ccg git tat gtg Pro Val Tyr Val 300 gga tic aag aac Gly Phe Lys Asn ggc ctg ctg ctg Gly Leu Leu Leu 335 gia cgc aic tac Vai Arg Ile Tyr 350 iig cag aic ggc Leu Gin Ilie Giy 365 ctc aac cag gaa Leu Asn Gin Glu 380 gca gcg gii agg Ala Ala Vai Arg 160 gaa Glu gig Val1 gga Gly gg a Gly gcc Al a 240 atc Ile tac Tyr cg t Arg gtc Val tig Leu 320 ati Ile aag Lys cat His aic Ile aag Lys 400 gic ggc acg acg Val Gly Thr Thr PCTIBOO/00926 165 tig 643 Leu gga Gly ica Se r tig Leu 225 a cc Thr aac As n gga Giy cgt Arg aca Thr 305 gig ValI gca Ala gaa Glu ga c Asp caa tic Phe aig Met 210 aic Ile gtg Val aat Asn atg Met cic Le u 290 gig Vai Gly gig Val icc Ser gga Gly 370 ga t aac Asn 195 iii Phe ctg Le u gaa Giu tig Le u atc Ile 275 act Thr ci g Le u tat Tyr aig Met gaa Glu 355 gcg Al a tca 180 cig aig Leu Met aac ccg Asn Pro gga tic Gly Phe gaa gtt Glu Vai 245 aac ccg Asn Pro 260 tic aac Phe Asn gcg aaa Ala Lys ati ggt Ile Gly gig tac Val Tyr 325 aig gig Mei Val 340 cgc cgc Arg Arg tig agi Leu Ser aac tig 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 Gin Asp Ser Asn Leu 385 tagitacict gcagggacga 1320 gat gag gaa caa ait aaa Asp Glu Glu Gin Ilie Lys 390 395 WO 01/00805 gct <210> 592 <211> 400 <212> PRT <213> Corynebacterium giutamicum PCTIBOOIOO926 1323 <400> 592 Met Ile Ile Gin Ile Leu 1 Val1 Ala Met Ala Se r Phe Leo Aia Thr 145 Val1 Val Giy Ser Leu 225 Th r Asn Gly Phe Ser Giy Lys Vai Pro Gly 130 Pro Gly Gly Phe Met 210 Ile Val1 Asn Aia Giy Vali Giu Ile Met Leu 115 Met Phe As n Tb r Asn 195 Phe Leo Glu Leu Gi y Ilie Met His Leo Phe 100 T rp Leo Ile Phe Thr 180 Leo Asn Giy Giu Asn 260 5 Phe Asp Aia Gi y Asp Aila Ile Asp Ile Se r 165 Leo Met Pro Phe Vai 245 Pro Ala Gly Leo Giu 70 Ile Giy Gly Val Ile 150 Se r Pro Vali Arg Leu 230 Giy Leo Arg Ser Ile Ile 55 Val1 Giy Ala Se r Asp 135 Phe Vali His Ala Val1 215 Ile Gin Ala Val Ala Phe 10 Gly Gin Gbu 25 Trp Gly Val 40 Ile Leu Gin Phe Arg Arg Val Val Val 90 Gly Ser Asn 105 Val Ile Met 120 Lys Vai Thr Ile Thr Ala Glu Gin Leo 170 Trp Ala Val 185 Val Ser Met 200 Ala Gly Arq Ile Ile Ser Asp Asp Met 250 Gly Gin Val 265 Ala Val Ile Leo Val1 75 Thr Leo Val Th r Ala 155 Asp Ala Ala Gly Ala 235 Pro Met Phe Met Val1 Gly Ser Leo Asn Leo Val 140 Ser Ser Ala Val Gly 220 Leo Met Ala Val1 Gin Ser Ser His Phe Gin Leo 125 Ile Ile Ala Val Val 205 Leo Thr Leo Val1 Gly Tyr Ala Tyr Pro Ser Gin 110 Val Giy Tyr Ala Asn 190 Ile Leo Leo Th r Val1 270 Ile Phe Val1 P he Val Thr Trp Leo Ala Tb r Leo 175 Tyr Gly Gly Phe Ile 255 Ile Ile ValI Ile Asn Phe Gly Gly Ala Ile Leo 160 Glu Val1 Gly Gly Ala 240 Ile Tyr Gi y Arg Th r 305 Val1 Ala Glu Asp Gin 385 WO 01/00805 Met Ile Phe 275 Leu Thr Ala 290 Val Leu Ilie Gly Tyr Vai Val Met Met 340 Ser Giu Arg 355 Giy Ala Leu 370 Asp Ser Asn Gly Arg Ser Giy Arg 345 Ala Le u Gin Met Phe Ph e T yr 330 Giy Asp Ala Ile Ph e Arg Vali 315 Ile Arg Le u Val Ly s 395 PCTIIBOO/00926 Arg ValI Le u 320 Ile Lys His Ile Lys 400 <210> 593 <211> 1323 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1300) <223> FRXA00842 <400> 593 ccttqtcgcg gaggtaagcg agggtatttc tggatgtgga tcgtgacttt cataacgttg agcctactaa ggtttgttcc cta Leu g ca Ala gga Giy ttg Le u gtg Vai ggg Giy t gg Trp att Ile ttc Phe atg Met gt t Val1 gga Gi y gt c ValI caa Gin tct Ser 45 agc Ser ggc Giy tat Tyr g ca Ala tat T yr atc Ile ttt Ph e gtg Val1 ttc Phe acaacgcgga ttatggaaaa atq att att caa atc Met Ilie Ile Gin Ile 1 gtt ggc gcc gqt ttc Val Gly Ala Giy Phe gcc ttc ggc ata gac Ala Phe Gly Ile Asp atg tcg gtg atq gcg Met Ser Vai Met Ala gca ggt gaa cac ggt Ala Giy Glu His Giy 115 163 211 259 307 WO 01/00805 WO 0100805PCTIIBOO/00926 gaa Giu atc Ile Gly g9t Giy gtg Val att Ilie 150 tca Se r cc t Pro gtt Vai C99 Ar 9 ttg Le u 230 99 C Gly ctg Le u a Cg Thr aac Asn ttt gtg Val1 99 C Gly gCg Al a tCt Ser ga t Asp 135 ttc Ph e gt 9 Val cac His 9 C9 Ala gtC Val 215 atc Ile caa Gin gCt Ala gca Ala cca Pro 295 9tg ttC Phe gt t Val 99a Gly gtg ValI 120 aaa Lys atc Ile gag Glu t 99 T rp gtg Val1 200 gca Al a atc Ile ga t Asp 9 C Giy ctg Leu 280 cag Gin t tg CgC Arg gtg Val tca Ser 105 atc Ile gta Val act Th r cag Gin gct Ala 185 tcc Ser ggt Gly atc Ile gat Asp caa Gin 265 ggt Gly cg a Arg agc cg a Arg gtg Val1 aat As n at 9 Met acc Th r 9CC Ala ctt Le u 170 gtt Val1 atg Met C99 Arg agt Ser atg Met 250 gta Val1 atg Met ttc Phe ttt gia Val1 75 a cg Thr ctg Leu gtt Vai aca Thr 9CC Ala 155 ga t Asp gca Ala 9ct Ala 99C Gly gC9 Al a 235 cct Pro atg Met ttC Phe c9 t Arg gtg agt Ser t tg Leu aat As n Ctt Leu gtc Val 140 t c9 Se r tct Se r 9C9 Ala gtg Val1 99 t Gly 220 cta Le u atg Met gca Ala tac Tyr C C9 Pro 300 gga caC His ttc Phe Cag Gin Ct 9 Leu 125 att Ile atc Ile 9Ct Al a gt 9 Val1 9 tc Val 205 t t9 Le u aca Th r ctg Le u gt9 ValI gca Ala 285 gt t Val1 ttc

CCC

Pro t cc Ser ca 9 Gin 110 9tg ValI ggt Gly taC Tyr 9 Ct Ala aac Asn 190 att Ile ctg Leu ctg Le u a Cg Th r gtt Val 270 ttg Leu tat Tyr aag qt t Val1 Thr 95 tqq Trp Ct 9 Leu gca Ala acg Th r tta Leu 175 tat Tyr 99 t Gly 99C Gly ttc Phe atc Ile 255 atc Ile 99C Gly gtg Val aac ttc Phe 80 ggt G ly 999 Gly 9 Ct Ala att Ile ct9 Le u 160 gaa Glu gt 9 Val1 gga Gly gga Gly 9CC Ala 240 atc Ile tac Tyr cgt Arg gtc Val1 t tg t CC Ser ttc Phe ct t Leu g9 Ala act Thr 145 gta ValI gtC ValI gga Gly tca Se r ttg Le u 225 acc Thr aac As n gga Gly Cgt Arg aca Thr 305 gtg aaa Lys gt c Val1 ccg Pro 99 C Gly 130 CCg Pro 99 t Gly 99 C Gly ttc Phe atg Met 210 atc Ile 9 t9 Val1 aat Asn a tg Met ctc Leu 290 gt 9 Val 99 C att ttg Ile Leu atg ttt Met Phe 100 ctc tgg Leu Trp 115 atg ttg Met Leu ttc atc Phe Ile aat, ttC Asn Phe acg acg Thr Thr 180 aac ctg Asn Leu 195 ttt aac Phe Asn ctg gga Leu Gly gaa gaa Glu Glu ttg aac Leu Asn 260 atc ttc Ile Phe 275 act gcg Thr Ala ctg att Leu Ile tat gtg ga c Asp gca Ala atc Ile 9a C Asp atc Ile agc Ser 165 ttg Le u atg Met CCg Pro ttc Phe gt t Val 245 cc9 Pro aac Asn aaa Lys g9t Gly tac 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 WO 01/00805 Phe Val Leu Ser Phe Val Gly Phe Lys 310 315 cca gtc ttg gga tac att. ggc ctg ctg Pro Val Leu Gly Tyr Ile Gly Leu Leu 330 gcg tgg gtg agg gga cgc gta cgc atc Ala Trp Val Arg Giy Arg Val Arg Ile 345 350 atg cgg atc gca gac ttg ttg cag atc Met Arg Ile Ala Asp Leu Leu Gin Ile 360 365 gga gca gag ctg gcg gtg ctc aac cag Gly Ala Giu Leu Ala Vai Leu Asn Gin 375' 380 gat gag gaa caa att aaa gca gcg gtt Asp Giu Giu Gin Ile Lys Ala Ala Vai 390 395 gct <210> 594 <211> 400 <212> PRT <213> Corynebacterium giutamicum <400> 594 Met Ilie Ilie Gin Ilie Leu Arg Val Ala PCTIBOOIOO926 Vai Gly Tyr Vai Tyr 325 gca gtg atg atg gtg 1123 Ala Val Met Met Val 340 gaa tcc gaa cgc cgc 1171 Giu Ser Giu Arg Arg 355 gac gga gcg ttg agt 1219 Asp Giy Ala Leu Ser 370 caa gat tca aac ttg 1267 Gin Asp Ser Asn Leu 385 tagttactct gcagggacga 1320 1323 1 Val Ala Met Ala Se r Phe Le u Ala Thr 145 Gi y Phe Ser Gly Lys Val1 Pro Gly 130 Gly Ile Met His Leu Phe 100 Trp Leu 5 Phe Asp Al a Giy Asp Ala Ile Asp Ala Gly Leu Giu 70 Ile Gly Gly Val Ser Ile Ile 55 Val Gly Al a Se r Asp 135 Gly T rp Ile Phe Val1 Gly Val 120 Lys Phe 10 Giu Val Gin Arg Val1 90 Asn Met Thr Ala Ala Val1 Ile Leu Val1 75 Th r Leu ValI Thr Ala 155 Ph e Met ValI Gly Ser Leu Asn Le u Val1 140 Se r Val1 Gin Ser Ser His Phe Gin Leu 125 Ile Ile Gly T yr Ala Tyr Pro Se r Gin 110 Val Gly Tyr Ile Phe Vai Phe Val Th r Trp Le u Ala Th r Ile Val1 Ile Asn P he Gly Gly Ala Ile Le u 160 Pro Phe Ile Ile Ile Phe Ile Thr 150 WO 01/00805 WO 0100805PCT/iBOO0926 Val Gly Asn Phe Ser Ser Val Giu Gin Leu Asp Ser Ala Ala Leu Glu 165 170 175 Val Gly Thr Thr Leu Pro His Trp Ala Val Ala Ala Val Asn Tyr Val 180 185 190 Giy Phe Asn Leu Met Vai Ala Val Ser Met Ala Val Val Ile Giy Gly 195 200 205 Ser Met Phe Asn Pro Arq Val Ala Gly Arg Gly Gly Leu Leu Gly Gly 210 215 220 Leu Ilie Leu Gly Phe Leu Ile Ile Ile Ser Ala Leu Thr Leu Phe Ala 225 230 235 240 Thr Val Giu Glu Val Gly Gin Asp Asp Met Pro Met Leu Thr Ile Ile 245 250 255 Asn Asn Leu Asn Pro Leu Ala Gly Gin Val Met Ala Val Val Ile Tyr 260 265 270 Gly Met Ile Phe Asn Thr Ala Leu Gly Met Phe Tyr Ala Leu Gly Arg 275 280 285 Arg Leu Thr Ala Lys Asn Pro Gin Arg Phe Arg Pro Val Tyr Val Val 290 295 300 Thr Val Leu Ilie Gly Phe Val Leu Ser Phe Val Gly Phe Lys Asn Let' 305 310 315 320 Val Gly Tyr Val Tyr Pro Val Let' Gly Tyr Ile Gly Leu Let' Leu Ile 325 330 335 Ala Val Met Met Vai Ala Trp Val Arg Gly Arg Val Ar Ilie Tyr Lys .340 345 350 Giu Ser Glu Arg Arg Met Arg Ile Ala Asp Leu Let' Gin Ile Gly His 355 360 365 Asp Gly Ala Let' Ser Gly Ala Glu Leu Ala Val Let' Asn Gin Glu Ile 370 375 380 Gin Asp Ser Asn Leu Asp Git' Glu Gin Ilie Lys Aia Ala Vai Arg Lys 385 390 395 400 <210> 595 <211> 1173 <212> DNA <213> Corynebacterium glutamictim <220> <221>*COS <222> (101)..(1150) <223> RXN00832 <400> 595 WO 01/00805 gagattgtqc taggttctga tgaggettcg ggacgaceg PCTIIBOO/00926 tgaeagcctg aagaaatcta ggaacggcce agagttctta agaaagtttg actagagaac atg ccg ttt eta Le u ttt Phe ate Ile gag Glu ace Th r ct g Leu gtg Val ggt Gly acc Thr C ca Pro 150 a Ca Thr gce Ala ga C Asp act Thr aaa Ly s atc Ile gtg Val1 cqt Arq ttg Leu ggg Gly tcc Ser gg t Gi y tac Tyr 135 gct Ala ct g Leu caa Gin gc a Al a gtg Val 215 eca Pro ate Ile qc a Al a ttg Le u teg Ser ce t Pro atg Met tta Leu 120 ttg Leu ttc Phe aca Thr get Ala gca Ala 200 etc Leu att Ile ccc Pro gte Val1 get Ala ate Ile get Ala ate Ile 105 agg Arg gt g Val1 agg Arg gea Ala gg t Gi y 185 aaa Ly s ccc Pro ga t Asp etc be u ate Ile cat His gtg Val ga t Asp ate Ile eat His et g Leo gga Gly gtg ValI 170 gaa Glu tgg Trp ate Ile tat Tyr ate Ile eta Leu att Ile ate Ile 75 tea Ser atg Met g ga Gly ate Ile gaa Glu 155 gtg ValI ttt Phe gag Glu gtg ValI gee Al a a ca Thr tte Phe ttg Leu 60 att Ile ace Thr gg t Gly age Se r gea Ala 140 tac T yr tac Tyr caa Gin gte ValI ttg Leo 220 ege Arg etg Leu tge Cys 45 gg t Gly gaa GiW act Thr t tg Leu atg Met 125 act Thr tee Se r ggg Gly gag G lu eca Pro 205 etg Leo ate Ile cca Pro 30 gea Ala ga t Asp gtg Val1 get Ala gte Val1 110 e ca Pro ttt Phe act Th r tte Phe gte Val1 190 ttt Phe tee Se r Met Pro 1 ttt gte ggc tgg Phe Val Gly Trp 15 tea att att gag Ser Ile Ile Glu ttt gge gtg gtg Phe Gly Val Val eet ttt gga teg Pro Phe Gly Ser att ttg ate tgt Ile Leo Ile Cys 80 ggt egg gat tee Gly Arg Asp Ser 95 gtg gga ttg tge Val Gly Leo Cys eae aat ggg gtg His Asn Gly Val 130 tee gta ate gee Ser Val Ile Ala 145 ggg eag gea ett Gly Gin Ala Leo 160 tte etg ttt cge Phe Leo Phe Arg 175 gag gte gca gaa Glu Val Ala Glu aga gge tta ate Arg Gly Leo Ile 210 eat gae atg gee His Asp Met Ala 225 Phe gea Al a ttg Leo aag Lys ttg Leo Ala gtg Val1 eta Leo 115 gga G ly ttt Phe gtt Val caa Gin aag Lys 195 ttg Leu tet tgg Ser Trp teg att Ser Ile geg ctg Ala Leo atg geg Met Ala ate ett Ile Leo gtg atg Val Met atg gea Met Ala 100 ete att Leo Ile aet eeg Thr Pro geg gtt Ala Val att tea Ile Ser 165 atg ggt Met Gly 180 gea gae Ala Asp att ate Ile Ile 115 163 211 259 307 355 403 451 499 547 595 643 691 739 787 aeg gtg atg Thr Val Met WO 01/00805 WO 0100805PCT/EBOO/00926 gaa Giu att Ile gcg Ala caa Gin gtg Val1 295 t tg Le u aag Ly s gtc Val1 gcc Ala tgg Trp gta Val1 280 atc Ile ct g Leu aaa Lys ctq Le u acc Thr aca Thr 265 tca Ser aca Th r ct g Leu gtc ValI gca Ala att Ile 250 gga Gi y acg Thr ggt Giy gga Giy aqt Se r 330 gca Ccc Ala Pro cca gag Pro Giu 255 cga gta Arg Val 270 aca atc Thr Ilie gtt ttg Val Leu gcg gtg Ala Val ggc tcg Gly Ser 335 gta Val 240 acc Th r agc Se r cca Pro ggg Giy aca Th r 320 qtg Val gca atg qct Ala Met Ala atc acc tcc Ile Thr Ser aac ctc gcg Asn Leu Ala .275 gct gtt cta Ala Val Leu 290 gag acc ccg Glu Thr Pro 305 gcc att gcg Ala Ile Ala cig ctc atg Leu Leu Met 835 883 931 979 1027 1075 1123 1170 1173 ggt gtt tac atg atg agc atg ttc gcc Gly Val Tyr Met Met Ser Met Phe Ala 345 350 ga a <210> 596 <211> 350 <212> PRT <213> Corynebacterium glutamicum <400> 596 Met Pro Phe Ser Trp Leu Lys Pro Ilie 1 5 Gly Trp Ala Ser Ilie Phe Ile Ile Pro Ile Glu Leu Ala Leu Ile Val Ala Val Val Val Lys Met Ala Glu Arg Leu Ala 55 Gly Ser Leu Ile Leu Thr Leu Ser Ilie 70 Ile Cys Ala Val Met Leu Gly Pro Ala Asp Ser Val Met Ala Val Ser Met Ile tgatttagigt agcctggtgg Asp Le u Ile His Val1 Asp Ile T yr Ile Le u Ile Ile 75 Se r Met Ala Th r Phe Le u Ile Th r Gi y Val1 Ile Gly P he Leu Arg Gily WO 01/00805 WO 0100805PCTIBOO/00926 100 105 110 Leu Cys Leu Leu Ile Gly Gly Leu Arg His Gly Ser Met Pro His Asn 115 120 125 Gly Val Gly Thr Pro Thr Tyr Leu Val Leu Ile Ala Thr Phe Ser Val 130 135 140 IleAla Phe Ala Val Pro Ala Phe Arg Gly Glu Tyr Ser Thr Gly Gln 145 150 155 160 Ala Leu Val Ile Ser Thr Leu Thr Ala Val Vai Tyr Gly Phe Phe Leu 165 170 175 Phe Arg Gin Met Gly Ala Gin Ala Gly Giu Phe Gin Giu Val Giu Val 180 185 190 Ala Giu Lys Ala Asp Asp Ala Ala Lys Trp Giu Val Pro Phe Arg Gly 195 200 205 Leu Ilie Leu Ile Ilie Thr Val Leu Pro Ile Val Leu Leu Ser His Asp 210 215 220 Met Ala Thr Val Met Asp Giu Val Leu Ala Ser Leu Gly Ala Pro Val 225 230 235 240 Ala Met Ala Gly Leu Ilie Ile Ala Thr Ile Val Phe Leu Pro Giu Thr 245 250 255 Ile Thr Ser Leu Lys Ala Ala Trp Thr Gly Giu Ile Gin Arg Val Ser 260 265 270 Asn Leu Ala His Giy Ala Gin Val Ser Thr Val Gly Leu Thr Ile Pro 275 280 285 Ala Val Leu Val Ile Gly Val Ilie Thr Gly Gin Asp Val Val Leu Gly 290 295 300 Giu Thr Pro Ile Asn Leu Leu Leu Leu Gly Thr Thr Ile Ala Val Thr 305 310 315 320 Ala Ilie Ala Phe Ser Ser Lys Lys Val Ser Ala Val His Gly Ser Val 325 330 335 Leu Leu Met Leu Phe Gly Val Tyr Met Met Ser Met Phe Ala 340 345 350 <210> 597 <211> 1119 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1096) <223> RXN00466 <400> 597 tttaaaagcg cactaagagc tcgtcaattc tttaaaacaa gctgagaatg tgaataatag WO 01/00805 gataggttaa cctgattcga PCTIBOOIOO926 caa tcc cgc ctg 115 Gin Ser Arg Leu ttagaaaacg gagatttgtc tcc Ser tta Le u tcC Se r acc Thr acc Th r 8cc Th r tta Leu ga t Asp tct Se r c cg Pro 150 gag Giu gga Giy ga t Asp tac Tyr ttc aaa Lys gct Ala a cg Th r acc Th r gac Asp gg a Gly gtg Val ctt Leu gcg Al a 135 gtg Val1 ga a Glu caa Gin gaa Glu Ca 9 Gin 215 ctc atc Ile ggg Gly gga Gly aca Thr gcc Al a tac T yr ga a Glu gaa Glu 120 ggg Gly gtc Val1 gct Ala gag Giu aat Asn 200 ggt Gi y ga t Ctg Leu t gt Cys aac Asn atc Ile ga t Asp aaa Lys gg c Gly 105 caa Gin ttt Phe gcc Ala a cc Th r ctc Leu 185 Cct Pro aaa Lys tca cgc Arg tct Se r t cc Ser gat Asp att Ile ttc Ph e aaa Lys gt a Val gac Asp cg t Arg aa c Asn 170 aat Asn tct Ser tac Tyr ctt agt agc Ser Ser aac aat Asn Asn gct. ttt Ala Phe gat gta Asp Val 60 gtC ctc Val Leu 75 ttc gaa Phe Glu gaa tta Glu Leu gca gcc Ala Ala gac gtt Asp Val 140 cca gcg Pro Ala 155 ctt gt t Leu Val gag gaa Glu Glu ttt gac Phe Asp ggt gcc Gly Ala 220 ggc att gtC Val gca Ala cct Pro 45 ccc Pro g ca Ala aac Asn aca T hr ctg Leu 125 gta ValI gga Gly gcc Ala aca Th r ggt Gi y 205 tac Tyr t cg gta Val ga t Asp 30 gtt Val1 gaa G iu t tg Leu gga Gly ctg Leu 110 gag Glu tac Tyr a ca Th r cgt Arg gat Asp 190 888 Lys ctg Le u ctg gg C Gly 15 ga c Asp t cg Ser aga Arg ggg Gly ttg Le u 95 ctt Leu cca Pro gag Glu gc t Ala gcg Ala 175 gct Ala aca Thr cca Pro ccg gtt Val1 8CC Thr att Ile gt t Val1 aCC Th r 80 gg a Gly gac Asp ga C Asp Caa Gin gca Ala 160 a tg Met ctg Leu g ga Gly ggc Gi y gaa gct Ala gaC Asp gaa Giu gt C Val1 gtc Val ccg Pro tct Se r ctg Le u cta Leu 145 tac Tyr ggg Gi y atc Ile 8CC Th.r ga t Asp 225 gca gt C Val1 gct Ala cac His 8CC Th r cca Pro t gg Trp ga t Asp att Ile 130 tct Se r gca Ala caa Gin caa Gin gt C Val1 210 gca Ala gtt cta Leu gat Asp gag Glu ctt Le u gta Val act Thr tcc Ser 115 att Ile ga t Asp gta Val t Ca Se r gct Ala 195 atc Ile Arg ctt gcc Ala t Ca Ser ttc Phe gg C Gly ggc Gly ga t Asp 100 aca Thr gga Gly atc Ile gct Ala gaa Glu 180 g cg Ala ttg Le u 998 Gly tcg ctg Leu aca Th r gga Gly gtt Val aac As n gag Gi u CCa Pro gt C Val1 gca Ala cgc Arg 165 888 Lys cg t Arg cca Pro caa Gin cga 163 211 259 307 355 403 451 499 547 595 643 691 739 787 835 Phe 230 gao Asp gao Asp ga t Asp aaa Lys tao Tyr 310 ogt Arg WO 01/00805 Leu Asp Ser acc ggo gao Thr Gly Asp gta gao ggt Val Asp Gly 265 atc aoa goa Ilie Thr Ala 280 gao goa gta Asp Ala Val 295 aao toa gtg Asn Ser Vai att got gag Ile Ala Giu Le u ago Se r 250 ga t Asp gag Giu att Ile otg Le u got Ala 330 Gly 235 tto P he gtt Val1 aat Asn gt g Val tot Se r 315 ttg Le u Ser Leu Pro Giu Ala Vai Leu 240 gtO gat gto 000 got gaa ago Val Asp Val Pro Ala Giu Ser 255 oto gtg ott too aao gao gaa Leu Val Leu Ser Asn Asp Giu 270 275 otg ttt gaa aoa oto aao gtt Leu Phe Giu Thr Leu Asn Val 285 290 aoa aoo gaa gaa ogo ggg gog Thr Thr Giu Giu Arg Gly Ala 305 oot ttt gog ttg gaa oat oto Pro Phe Ala Leu Giu His Leu 320 taaaaotoaa otaotogago aoa PCT1B00/00926 Arg 245 aaa 883 Lys otg 931 Le u oaa 979 Gin aoo 1027 Th r ooa 1075 Pro 325 1119 <210> 598 <211> 332 <212> PRT <213> Corynebaoterium giutamioum <400> 598 Val Gin Ser Arg Leu Ser Lys Ilie Leu 1 5 Ala Val Leu Ala Leu Leu Ala Gly Cys Asp Ala Asp Ser Thr Ser Thr Gly Asn 40 Giu His Giu Phe Gly Thr Thr Thr Ile 55 Val Thr Leu Gly Val Thr Asp Ala Asp 70 Val Pro Val Gly Asn Thr Gly Tyr Lys Pro Trp Thr Asp Giu Leu Val Giu Gly 100 105 Ser Asp Ser Thr Pro Asp Leu Giu Gin 115 120 Leu Ile Ile Gly Val Ser Ala Gly Phe 130 135 Arg 10 Se r Ser Asp Ile Phe 90 Lys Val1 Asp Ser As n Ala Asp Val 75 Phe Giu Al a Asp Ser Asn Phe ValI Leu Giu Leu Ala Val 140 Vali Ala Pro Pro Ala Asn Th r Le u 125 Val Gly Asp Ser Arg Gly Le u Leu Pro C iu Val1 Th r Ile Val Thr Gly Asp Asp Gin WO 01/00805 PCT/IBOO/00926 Leu Ser Asp Ile Ala Pro Val Val Ala Arg Pro Ala Gly Thr Ala Ala 145 Tyr Gly Ile Thr Asp 225 Ala Ala Asn Le u Arg 305 Glu Ala Gin Gin Val 210 Ala Val Giu Asp Asn 290 Gi y His Arg 165 Lys Arg Pro Gin Arg 245 Lys Leu Gin Thr Pro 325 150 Giu Gly Asp Tyr Phe 230 Asp Asp Asp Lys Tyr 310 Arg Ala Thr Gly 205 Tyr Se r Val1 Le u Leu 285 Thr Pro Arg Asp 190 Lys Le u Le u Asp Val1 270 Phe Th r Phe Ala 175 Ala Th r Pro Pro Val 255 Leu Glu Glu Ala 160 Met Le u Gly Gly Giu 240 Pro Se r Thr Glu Le u 320 <210> 599 <211> 1395 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1372) <223> RXN01936 <400> 599 gcgcggtqac accacagccg ttqtcagcgg cgcttggtct gtggaggatc gccgaggtta ctaacaaata gqcccaacaa agaggtctaa gctctacctg gtg agt ttc cga gat 115 Val Ser Phe Arg Asp 1 att ttc gct gac acc aga ccg ctg aaa gaa ccg gcc ttc aaa cgc ctc 163 Ile Phe Ala Asp Thr Arg Pro Leu Lys Giu Pro Ala Phe Lys Arg Leu 15 tgg ctt ggc aat gtt gcc acc gtc att ggt gcc caa tta act gtt qtt 211 Trp Leu Gly Asn Val Ala Thr Val Ile Gly Ala Gin Leu Thr Val Val 30 WO 01/00805 gcc gtt ccg qtg Ala Val Pro Val ggc ttg acc gg Gly Leu Thr Giy tat ggt gga tcc Tyr Gly Gly Ser tgc acc acg atc Cys Thr Thr Ile acc att tta ggc Thr Ilie Leu Gly 105 tta cog cag gca Leu Gin Gin Ala 120 ctt cga agt ott Leu Arg Ser Ile 135 oat atg ctg ctc Asn Met Leu Leu 150 ggt gcg ttg att Gly Aia Leu Ile gtt gtc tcc atc Vai Val Ser Ile 185 otc aag cca tcc Ile Lys Pro Ser 200 gat ggc ctg aag Asp Gly Leu Lys 215 gtg ctg gat ctt Val Leu Asp Leu 230 ccc gag atc gca Pro Glu Ile Ala atg ctg gcg ttc Met Leu Ala Phe cog Gin ctt Leu att Ile Gly aat Asn ttt Phe t tg Le u atg Met ccg Pro 170 atc Ilie gg C Gi y tat Tyr atc Ile ga Glu 250 atg Met ott Ilie ttt Phe gcg Ala 75 at g Met gag Giu ttc Phe c cg Pro co g Gin 155 ctg Leu ccc Pro 00 g Lys ttg Leu gcc Ala 235 gtg Vol tac Tyr g gc Gly 60 got Asp t gt Cys oat Asn gcg Ala Ott Ile 140 acc Th r at c Ile aca Thr gtg Val gct Ala 220 otg Met oac As n coo Gin 45 Ct t Leu gc t Ala gtc Vol ott Ile gtg Val1 125 got Asp gq c Gly ggt Gly ctg Le u atg Met 205 ggc Gly ott Ilie ttc Phe o tg Met ott Ile ttt Phe occ Th r tgg T rp 110 oat As n coo Gin gc a Ala ttc Phe tgg Trp 190 aag Lys coo Gin ttc Phe gg t Gly oct Th r cct Pro got Asp act Thr 95 ctc Leu coo Gin tto Leu otc Ile ggg Gly 175 gct Ala gct Ala ccc Pro ggc Gly ggg Gly 255 gct Ala ggg Gl y ttg Leu 000 Lys 80 gcc Ala ctg Leu ccc Pro g cg Ala gt t Vol 160 tgg Trp gto Vol ggt Gly qtg Val1 atg Met 240 gg t Gly tcc Ser gtt Vol cg c Arg ggt Gly tta Le u acc Thr tcg Ser 145 ggc Gly ctg Leu tgg Trp ttc Phe ttg Le u 225 cc a Pro goc Asp t cc Se r Ott Ile atc Ile ttt Phe ata Ile cga Arg 130 g ca Ala ccg Pro tot Tyr tco Ser gcc Alo 210 ttg Le u cg t Arg gcc Ala ggc tot Gly Tyr ttt ggc Phe Gly gtg ctg Val Leu tgg gtg Trp Vol 100 oac ttt Asn Phe 115 ocg gcg Thr Ala oca tca Thr Ser ctg atc Leu Ile ttc ctt Phe Leu 180 ctg cct Leu Pro 195 agt gtg Ser Vol otg gtg Met Vol gcg ctt Ala Leu ggt gca Gly Alo 260

PC

gtg Vol ctt Leu atc Ile ctg Le u tct Ser atc Ile ctg Leu gca Ala 165 got Asp t cg Ser gtg Vol atg Met tac T yr 245 o cg Th r T11B00100926 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 toc tco tcc atg Tyr Ser Ser Met 270 gtt ggc gca gtt ctt ggc Vol Gly Ala Vol Leu Gly 275 265 WO 01/00805 WO 0100805PCTIEBOOIOO926 9gC Gly gtt Val gtg Val1 310 ttc Phe gca Ala ggc Gly tta Leo t gg Trp 390 t gt Cys acc Thr 9 tg Val1 tat Tyr 295 gca Ala atc Ile gtt Val1 cga Arg gct Ala 375 a cg Th r a tg Met ggC Gi y ctg tct ggt Leu Ser Gly 280 tgg tgc atc Trp Cys Ile att gtt gtc Ilie Val Val atc atg atg Ilie Met Met 330 cga aac gct Arg Asn Ala 345 atc caa ggt Ilie Gin Gly 360 gac gtc ctt Asp Vai Leu gta tta tgg Val Leo Trp gtg gcg gtg Val Ala Vai 410 atc taaatacl Ile tgg Trp atc Ile agc Se r 315 gtc Val1 at t Ile gtg Vai cac His gqc Giy 395 cc t gtg Val gCC Ala 300 ccc Pro att Ilie ttg Leo t gg Trp gq t Gi y 380 gga Gly aaa 9C Ala 285 t gg Trp 99C Gly gq t Gly ca g Gin at c Ile 365 tgg Trp qta Val ttc Phe cgg Arg 9gc Giy gcg Ala 9gC Gly cag Gin 350 atc Ile 9CC Ala gcg Ala t gg Trp agc Ser g cc Ala act Th r 320 gct Ala gct Ala gt 9 Val gag Glu gtt Val1 400 tac Tyr cgc Arg gtt Val 305 gcg Ala gac Asp gcg Ala gtg Val1 ccc Pro 385 gta Val1 gag Glu g ca Ala gg t Gly atg Met 325 t cg Se r ca g Gin cg t Arg 99 t Gly att Ile 405 att Ile 979 1027 1075 1123 1171 1219 1267 1315 1363 1395 Pro Lys 420 tta tccatgccca ttt <210> 600 <211> 424 <212> PRT <213> Corynebacterium giotamicum <400> 600 Val Ser Phe Arg Asp Ile Phe Ala Asp 1 5 Ala Phe Lys Arg Leo Trp Leo Gly Asn 25 Gin Leo Thr Val Val Ala Val Pro Val 40 Ser Ser Gly Tyr Val Gly Leo Thr Giy 55 Vai Ile Phe Gly Leo Tyr Gly Gly Ser 70 Thr 10 Val1 Gin Leo Ile Arg Pro Leo Lys Glu Pro Ala Thr Val Ile Gly Ala Ile Tyr Gin Met Thr Gly Phe Gly Leo Ile Pro Leo Ala Asp Ala Phe Asp Lys 75 WO 01/00805 PCT/IB00/00926 Arg Ile Val Leu Ile Cys Thr Thr Ile Gly Met Cys Val Thr Thr Ala 90 Gly Phe Trp Val Leu Thr Ile Leu Gly Asn Glu Asn Ile Trp Leu Leu 100 105 110 Leu Ile Asn Phe Ser Leu Gin Gin Ala Phe Phe Ala Val Asn Gin Pro 115 120 125 Thr Arg Thr Ala Ile Leu Arg Ser Ile Leu Pro Ile Asp Gin Leu Ala 130 135 140 Ser Ala Thr Ser Leu Asn Met Leu Leu Met Gin Thr Gly Ala Ile Val 145 150 155 160 Gly Pro Leu Ile Ala Gly Ala Leu Ile Pro Leu Ile Gly Phe Gly Trp 165 170 175 Leu Tyr Phe Leu Asp Val Val Ser Ile Ile Pro Thr Leu Trp Ala Val 180 185 190 Trp Ser Leu Pro Ser Ile Lys Pro Ser Gly Lys Val Met Lys Ala Gly 195 200 205 Phe Ala Ser Val Val Asp Gly Leu Lys Tyr Leu Ala Gly Gin Pro Val 210 215 220 Leu Leu Met Val Met Val Leu Asp Leu Ile Ala Met Ile Phe Gly Met 225 230 235 240 Pro Arg Ala Leu Tyr Pro Glu Ile Ala Glu Val Asn Phe Gly Gly Gly 245 250 255 Asp Ala Gly Ala Thr Met Leu Ala Phe Met Tyr Ser Ser Met Ala Val 260 265 270 Gly Ala Val Leu Gly Gly Val Leu Ser Gly Trp Val Ala Arg Ile Ser 275 280 285 Arg Gin Gly Val Ala Val Tyr Trp Cys Ile Ile Ala Trp Gly Ala Ala 290 295 300 Val Ala Leu Gly Gly Val Ala Ile Val Val Ser Pro Gly Ala Val Thr 305 310 315 320 Ala Trp Ala Trp Met Phe Ile Ile Met Met Val Ile Gly Gly Met Ala 325 330 335 Asp Met Phe Ser Ser Ala Val Arg Asn Ala Ile Leu Gin Gin Ser Ala 340 345 350 Ala Glu His Val Gin Gly Arg Ile Gin Gly Val Trp Ile Ile Val Val 355 360 365 Val Gly Gly Pro Arg Leu Ala Asp Val Leu His Gly Trp Ala Ala Glu 370 375 380 Pro Leu Gly Ala Gly Trp Thr Val Leu Trp Gly Gly Val Ala Val Val 385 390 395 400 WO 01/00805 PCT11EB00100926 Val Leu Thr Ala Ilie Cys Met Val Ala Val Pro Lys Phe Trp Lys Tyr 405 410 415 Giu Lys Pro Lys Ile Thr Gly Ile 420 <210> 601 <211> 1461 <212> DNA <213> Corynebacterium giutanicun <220> <221> CDS <222> (1438) <223> RXN01995 <400> 601 ccqacgcaaa ggcatgcgcc tgcgtgtctc gagtagtctc cctcgaccat tacggtcgcc ctctcctaaa ggagcctggc ctccccttcc tcgtccccaa atg gat Met Asp atc cgc caa Ile Arg Gin aca Thr atc Ilie tct Se r ca g Gin tct Se r ct 9 Le u acc Th r atc Ile ttc Phe att Ilie qca Aia ttt Phe ctt Le u t tg Leu atg Met gcg Aia Giy tcc Se r aac Asn gtg Vali act Thr ggt Giy ctg Le u atc Ile cag Gin at c Ile 120 aac Asn ga c Asp ctg Leu gcg Aia gtg Vai ttc Phe gCg Ala t CC Se r 105 99C Giy aac As n aca Thr ctc Leu aat Asn ctg Leu gg t Giy ct g Le u g ca Ala gq a Gly aaa Ly s gca Al a aac As n gca Al a ctg Leu ccg Pro 75 ctg Leu 99C Gly atc Ile aac Asn atg Me t gca Ala gtg Val agi Ser 60 atc Ile ttc Phe ca g Gin ct C Le u cg C Arg tcg Se r ctg Leu acc Thr 45 tcc Ser ggt Gly aac As n t tg Leu gcc Ala 125 ggC Gly tat Tyr 15 9qc Gly gaa Giu ctg Leu cg t Arg gtg Val1 95 qtg Val1 atc Ile qCC Ala ggt ca 9 Gin ttt P he ttt Phe t tc P he ttc Phe 80 gga Gly tg Trp aca Thr atg Met ttc 1 tqg Trp gat Asp gga Gly gg C Gly ggC Gly t tg Le u cgt Arg gt q Val1 tcc Se r 145 ggC ttc P he gtc Val1 ctg Leu atg Met cgt Arg gta Val1 ttg Le u qtg Val1 130 atc Ile gca att Ile ctc Leu agt Ser acc Th r a ag Lys ttg Leu atc Ile 115 atc Ile tac Tyr gcq gta Val1 gcc Al a ggC Gly gc t Ala aat Asn tcc Ser 100 act Th r agt Se r gct Ala C ag ttt Ph e atg Met agc Ser gga Gly gCC Ala gcc Ala gt Gly gag Glu gct Ala ctc 115 163 211 259 307 355 403 451 499 547 595 135 140 ggt tac ggc atc ggc gcg tcc ttg ggc WO 01/00805 PCTABOO/00926 Gly 150 atc Ile act Th r ga t Asp tac Tyr gaa Giu 230 ctc Leu ttc Phe aag Lys ggt Gly ttc Ph e 310 g tg ValI cca Pro gg t Gly gaa Giu Tyr Gly Ile Gly Ala Ser Leu Gly Gly Phe Gly Ala Ala Gin Leu cca Pro gg t GI y tg Trp att Ile 215 caa Gin gt t Val1 gg C Gly ct g Le u ttg Leu 295 ctc Leu ctg Leu tcc Ser tg t Cys gta ValI 375 aca Thr atc Ile ctg Le u 200 g cg Aia a gc Ser aac As n atc Ile ctc Leu 280 atg Met acc Thr tcc Se r atc Ile gtg Val1 360 cg c Ar g 155 ttt gga tgg Phe Gly Trp 170 gcc acc atc Ala Thr Ile 185 agc act cgc Ser Thr Arg cgc cgc ctg Arg Arg Leu ttg tcg aeg Leu Ser Thr 235 aaa gag aac Lys Giu Asn 250 gtg atq ttc Vai Met Phe 265 gtg gaa acc Val Giu Thr ttg tcc atg Leu Ser Met acc aag ttc Thr Lys Phe 315 ggc ctg acg Gly Leu Thr 330 gcg ttt gcc Ala Phe Ala 345 gct ggt ctg Ala Gly Leu acc act ggt Thr Thr Gly 160 165 cgc Arg gct Ala cgc Arg ggc Gly 220 aaa Lys cgt Arg ggc Gly 9ga Gly gg t Gly 300 a gc Ser ctg Leu a gc Ser tac Tyr gtg Val 380 tcc Ser act Thr cc t Pro 205 aaa Lys aaa Lys gqa Gly ttc Phe at g Met 285 ga Gly t cc Ser atc Ile Gly acc Th r 365 gg C Gly gtg ttc Val Phe 175 ttc ttc Phe Phe 190 gcg ggc Ala Gly gtc ggt Val Gly gcc ggt Ala Gly acc agc Thr Ser 255 tac ttc Tyr Phe 270 tca gaa Ser Giu gca ttc Ala Phe cga aac Arg Asn ctg ttc Leu Phe 335 gtt gtc Val Val 350 ctg tcc Leu Ser gct gcg Ala Ala gtg ggt Val Gly gca Ala ttc Phe gc t Al a acc Thr ctc Leu 240 atc Ile qcc Ala ca g Gin Gi y aca Th r 320 att Ile gtg Val cca Pro att Ile gcc Al a ctg Le u cq c Ar g ttt Phe 225 caa Gin aag Ly s aac Asn ca g Gin tcc Se r 305 ct g Le u tCC Se r ggc Gly ca g Gin ggt Gly 385 ggt Gly cca Pro gac Asp 210 gag Glu tcg Ser ctg Leu act Th r 9gc Gly 290 ctg Le u atg Met tcc Se r a tg Met ct g Le u 370 atg Met gcg Al a gaa Giu 195 aag Lys ctt Leu tat Tyr t gg T rp tgg Trp 275 atc Ile ctc Leu acc Th r acc Thr ct g Leu 355 tac Tyr ggt Gly atc Ile 180 tcc Ser atc Ile cca Pro gca Ala gt t Val1 260 acc Th r atc Ile tac Tyr ttc Phe tct Ser 340 atc Ile t cc Ser cgt Arg g ca Ala gtt Val1 aat Asn ggc Gly gtg ValI 245 gcg Ala ccg Pro ggt Gly gg 9t Gly atg Met 325 g tt Val1 aat Asn gc t Ala gtc Val1 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1315 ggt gcg att tcc gcg Gly Ala Ile Ser Ala cca ctg ctg Pro Leu Leu ggc ctg ctg gat tct ggc Gly Leu Leu Asp Ser Gly WO 01/00805 PCT/IBOO/00926 390 395 400 405 tgg tcc cca acg cag ctg tat gtt ggt gtg gca gtg att gtt att gcC Trp Ser Pro Thr Gin Leu Tyr Val Gly Val Ala Val Ile Val Ile Ala 410 415 420 ggt gca acc gca ttg att ggg atg cgc act cag qcg gta gcc gtc gaa Gly Ala Thr Ala Leu Ile Gly Met Arg Thr Gin Ala Vai Ala Val Glu 425 430 435 aag cag cct gaa gcc cta gcg acc aaa tagggccgcg attcctagca Lys Gin Pro Giu Ala Leu Ala Thr Lys 440 445 tgc <210> 602 <211> 446 <212> PRT <213> Corynebacterium giutanicum 1363 1411 1458 1461 <400> 602 Met Asp Ile Arg Gin Thr Ile Asn Asp 1 Trp Asp Gly Gly Gly Leu Arg Val Ser 145 Gly Ala Phe Val Leu Met Arg Val Leu Val 130 Ile Ala Gly Ile Leu Ser Thr Lys Leu Ile 115 Ile Tyr Ala Ala Val Ala Gly Ala Asn Ser 100 Thr Ser Ala Gin Ile 180 5 Phe Met Ser Gly Ala Ala Gly Glu Ala Leu 165 Ala Ile Ser Gin Ser 70 Leu Thr Ile Phe Gly 150 Ile Thr Ala Phe Leu 55 Leu Met Ala Gly Ser 135 Tyr Pro Gly Va1 Thr 40 Gly Leu Ile Gin Ile 120 Asn Gly Thr Ile Leu 25 Ala Va1 Phe Ala Ser 105 Gly Asn Ile Phe Ala 185 Thr 10 Leu Asn Leu Gly Leu 90 Ala Gly Lys Gly Gly 170 Thr Ala Asn Ala Leu Pro 75 Leu Gly Ile Asn Ala 155 Trp Ile Met Ala Val Ser Ile Phe Gin Leu Arg 140 Ser Arg Ala Ser Leu Thr Ser Gly Asn Leu Ala 125 Gly Leu Ser Thr Arg Asp Glu Ala Asp Va1 Gly 110 Cys Met Gly Va1 Phe 190 Tyr Gly Glu Leu Arg Val Val Ile Ala Gly Phe 175 Phe Gin Phe Phe Phe Phe Gly Trp Thr Met Phe 160 Ala Phe Leu Pro Glu Ser Val Asp Trp Leu Ser Thr Arg Arg Pro Ala Gly Ala WO 01/00805 PTIO/02 PCTABOO/00926 Arg Asp Lys Ile Asn Tyr Ile Ala Arg Arg Leu Gly Lys Val Gly Thr 210 215 220 Phe Giu Leu Pro Gly Giu Gin Ser Leu Ser Thr Lys Lys Ala Gly Leu 225 230 235 240 Gin Ser Tyr Ala Val Leu Val Asn Lys Giu Asn Arg Gly Thr Ser Ile 245 250 255 Lys Leu Trp Val Ala Phe Gly Ile Val Met Phe Gly Phe Tyr Phe Ala 260 265 270 Asn Thr Trp Thr Pro Lys Leu Leu Val Gil. Thr Gly Met Ser Giu Gin 275 280 285 Gin Gly Ile Ile Gly Gly Leu Met Leu Ser Met Gly Gly Ala Phe Gly 290 295 300 Ser Leu Leu Tyr Gly Phe Leu Thr Thr Lys Phe Ser Ser Arg Asn Thr 305 310 315 320 Leu Met Thr Phe Met Val Leu Ser Gly Leu Thr Leu Ile Leu Phe Ile 325 330 335 Ser Ser Thr Ser Val Pro Ser Ile Ala Phe Ala Ser Gly Val Vai Val 340 345 350 Gly Met Leu Ile Asn Gly Cys Val Ala Gly Leu Tyr Thr Leu Ser Pro 355 360 365 Gin Leu Tyr Ser Ala Giu Val Arg Thr Thr Gly Vai Gly Ala Ala Ile 370 375 380 Gly Met Giy Arg Val Giy Ala Ile Ser Ala Pro Leu Leu Val Gly Gly 385 390 395 400 Leu Leu Asp Ser Gly Trp Ser Pro Thr Gin Leu Tyr Vai Gly Val Ala 405 410 415 Val Ile Val Ile Ala Gly Ala Thr Ala Leu Ile Giy Met Arg Thr Gin 420 425 430 Ala Val Ala Val Giu Lys Gin Pro Glu Ala Leu Ala Thr Lys 435 440 445 <210> 603 <211> 813 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(790) <223> RXN00661 <400> 603 cggatgcaag agaaccgtgg tttcgctgat ttttggcgaa cccggaatta aggccccgag gattacatgc ttttaaatcc tttgaaaagg ggacaagatc atq aat cct ata acc 115 WO 01/00805 gaa Giu cgc Arg a tg Met ctg Le u ctc Le u agt Ser acc Th r ga a Giu gcc Ala t gq T rp 150 tgg Trp cct Pro gtg Val1 ca a Gin ta Leu gaa Giu cga Arq ctg Leu ga t Asp ggt Gly cca Pro cgc Arg t gg T rp 135 att Ile aca Thr cta Leu tac T yr aaa Lys tta be u atc Ile cg c Arg ttc Phe ct C be u tat Tyr gaa Giu g cc Aila 120 att Ile ttc Phe gag Giu ctt Leu gq t Giy 200 gca Ala ga c Asp atc Ile atc Ile aca Th r tac Tyr ggc Gi y aat As n 105 gg C Gly ttc Phe gtc Val1 ttc Ph e t tg be u 185 gc g Aila gac Asp gca aca cta tgg atc ggc Ala Thr Leu Trp Ile ggc aac Giy Asn gtg tgg Val Trp gta ttt Val Phe qgc caa Gly Gin 75 tgg tac Trp Tyr gca gta Ala Vai att gtg Ile Val caa gca Gin Ala ggc tca Gly Ser 155 tgg ctg Trp Leu 170 act gct Thr Ala ttt gtc Phe Val aag gct Lys Ala gtt Val1 gca Ala a tg Met 60 gcg Ala caa Gin gca Ala att Ile ctc be u 140 atc Ile atc Ile Gly agc Ser cgt Arg 220 ttc Phe tgg Trp 45 ggC Gly ggt Gly t gg Trp gtg Val gcg Ala 125 ggc Gly ct q be u tgg Trp tac Tyr t gg Trp 205 g cg Al a g9a Gly 30 ccc Pro ggc Gly cg c Arg t cg Ser gt t Val1 110 g cg Ala tcc Ser gct Ala atc Ile tac Tyr 190 gga Gly Gly tta Leu atc Ile ctt Leu ca g Gin gcc Al a 95 cct Pro gtt Val tgg Trp acc Th r gcc Ala 175 cca Pro ttt Phe Met 1 gga gtt Gly Val ttt agc Phe Ser ggc atc Gly Ile ttc cac Phe His atc atg Ile Met 80 gca aaa Ala Lys cgc tgg Arg Trp gtg gga Val Gly ggg cca Gly Pro 145 tac gga Tyr Gly 160 gtc gac Val Asp tcc gcg Ser Ala gtc gtg Val Val Asn C cg Pro gcg Ala ata Ile act Thr ttc Phe cgt Arg gca Ala a ca Thr 130 t gg Trp atg Met ata Ile gt g Val1 t gg Trp 210 Pro att Ile tgg Trp gqC Gi y cca Pro atc Ile cg C Arg ag c Se r 115 ctc Le u gcc Al a gct Ala gt t Val1 ctt Leu 195 ctg be u Ile ctg be u g ca Ala aac Asn caa Gin atc Ile gca Ala 100 acc Th r agc Se r gac Asp cgc Arg ggc Gi y 180 tac T yr c99 Arg PCTIBOOIOO926 Thr tgg 163 Trp gga 211 Gly gcg 259 Ala aac 307 Asn gtc 355 ValI ctc 403 Le u aaa 451 Lys ttt 499 Phe gcg 547 Ala qga 595 Gly 165 gtt 643 Val ctg 691 be u gtg 739 Val ctg gaa gct cag gag beu Giu Ala Gin Glu 225.

tct gtg aca Ser Val Thr 215 gt c Val1 tgaaaagcgt ttactaaata gaa wo 01/00805 WO 0100805PCT/IBOO/00926 230 <210> 604 <211> 230 <212> PRT <213> Corynebacterium giutamicum <400> 604 Met Asn Pro Ilie Thr Giu Leu Leu Asp 1 5 Val Pro Ilie Leu Trp Arg Giu Ile I Ser Ala Trp Ala Giy Met Arg Arg I 40 Ile Ilie Gly Asn Ala Leu Leu Phe 'I 55 His Thr Pro Gin Asn Leu Asp Leu T Met Phe Ilie Ilie Vai Ser Gly Tyr C Lys Arg Arg Aia Leu Thr Pro Giu P 100 1 Trp Ala Ser Thr Lys Giu Arg Ala C 115 -120 Gly Thr Leu Ser Phe Ala Trp Ile F 130 135 Pro Trp Ala Asp Ala Trp Ile Phe 145 150 Gly Met Ala Arg Gly Trp Thr Giu E 165 Asp Ilie Val Giy Val Pro Leu Leu 1 180 2 Ala Val Leu Tyr Leu Vai Tyr Gly .7 195 200 Val Trp Leu Arg Vai Gin Lys Ala 1 210 215 Gin Glu Ser Val Thr Val 225 230 <210> 605 <211> 1332 <212> DNA <213> Corynebacterium glutamicum <220> Ala Gly Vai Vali Gly Trp Ala Ile Gin Gly Trp 170 Thr Ph e Le u ValI Ala Met Ala Gin Ala Ile Leui 140 Ile I le Gly Se r Arg 220 Trp Phe Trp Gly Gly Trp Val1 Al a 125 Gly Le u Trp T yr Trp 205 ~sp Lys Ala Ala Leu Giu Ala WO 01/00805 <221> CDS <222> (1309) <223> RXN02566 <400> 605 gactccggat gatcaagcag cgaaaccttt gtcaagaaca PCTJIBOO/00926 tagctcccca ccctagacaa aagcccaaat aaatctgctc aataacctaa cctaaagtcc atg cac gcc tcg tcg Met His Ala Ser Ser ccc Pro gcc Ala ag t Ser gc C Ala cq C Arg ttg Leu 9gc Gly t cg Se r gaa Glu atc Ile 150 agc Se r tta Leo aaa Lys ca a Gin caa Gin ttg Leo aca Th r atg Met ct t Leo ttg Leo g9 Ala gaa Glu 135 gqc Gly gaa Glu ttt Phe ggc Gly cct Pro atc Ile ctg Leo ttg Leo gtc Val1 gg t Gly ttt Phe gtt Val 120 acc Tb r gca Ala acc Thr ggc Gi y ctc Leu cac His atg Met gca Ala ac c Th r t cc Se r t 9C Cys cca Pro 105 aac Asn cg C Arg atc Ile ctg Leo ca g Gln 185 aaa Lys cct Pro gt t Val1 qca Ala a cg Thr aca Thr gtg Val gta Val ctc Leo Gly gcc Ala ggc Gly 170 ct C Leo ccc Pro c ag Gin ggt Gly cac His atc Ile tac Tyr 75 99C Gly gta Val caa Gin cgc Arg qga Giy 155 ctc Leo atc Ile gaa Glu cgc Arg g ca Ala t tg Leo Gly 60 gat Asp g ca Ala Ct t Leo gca Ala gac Asp 140 cca Pro gaa Glu gcc Ala gc C Ala acc Tb r t cc Ser gcg Ala 45 gca Al a cgc Arg cta Leo t tg Leo cgt Arg 125 Ct C Leo aat As n ca a Gin atc Ile acc Th r cgt Arg aat As n 30 qq a Gly gc t Al a cga Arg ctg Leo gca Ala 110 ttC Phe t cg Se r tta Leo cat His gca Ala 190 cca Pro gtt Val 15 99c Gly gct Ala atc Ile act Tb r g cg Ala 95 ttt Phe gcc Ala atc Ile ttt Phe g cc Ala 175 gtc Val1 aa t Asn ct c Leo gtg Val t cg Ser ttt Phe t ca Ser 80 atc Ile t tg Leo gca Al a gt t Val gaa Glu 160 gg c Gly t gg Trp gc a Al a 1 agc Ser acg Th r tgg T rp tcg Se r ctc Leo ctc Leo ttc Phe acc Thr gtg Val1 145 cca Pro gca Ala cga Arg cca Pro gg c Gly cta Leo gga Gly att Ile agc Ser ggc Gly ctc Leo gac Asp 130 tgg Trp agc Se r tac Tyr ttc Ph~e aca Th r ctg Leo t cg Se r gga Gly ccc Pro a cg Th r gca Al a gga Gl y 115 qt 9 Val1 tcc Se r gcc Ala c tg Leo acc Thr 195 gaa Glu att Ile a tgc Met tca Se r ctt Leo Gly caa Gln 100 t cc Se r gcc Ala a cc Thr cga Arg ctg Leo 180 ct c Leo aag Ly s ttc Ph e gga Gb y gcc Ala gcc Ala atg Met ttc Phe atg Met agt Ser acc Thr ttc Phe 165 tgt Cys ccc Pro a ag Lys 115 163 211 259 307 355 403 451 499 547 595 643 691 739 WO 01/00805 WO 0100805PCT[IBOO/00926 C9c Arg cac His 230 ggc Gly gtc Val1 a aa Lys aca Thr atc Ile 310 99 t Gi y tac Tyr 99c Gi y ttig Le u ttc ctc Leu 215 ttc Phe cac His qcc Al a ctc Le u t cc Ser 295 aca Th r tct Se r gCC Al a qg 9 Gi y gca Al a 375 aga 200 acc Thr t CC Se r ggc Gly gga Gi y 99 C Gi y 280 9 ca Ala tCC Ser t ca Ser cag Gin ttg Le u 360 99C Gly acc ccg Pro atg Met gcc Ala atg Met 265 cgc Ar9 g ca Ala atg Met aca Thr 999 Gly 345 atc Ile gtC Val1 c9 t aaa Lys 9t C Val1 agc Ser 250 tac Tyr aat Asn ttt Phe atc Ile t t9 Leu 330 cg C Arg gcc Ala gt t Val1 t ca gcc Ala 99t Gly 235 ct C Leu qca Ala gtc Val1 ctt Le u ctg Le u 315 ctt Leu agc Se r gga Gly ctt Le u att ctc Leu 220 ctc Leu acc Thr ctc Leu a cc Thr atc Ile 300 ctt Leu gtC ValI gac Asp ccg Pro gca Ala 380 ga a 205 caa Gin atg Met atc Ile t ca Ser atc Ile 285 at t Ile gggj Gly ga c Asp cta Leu tta Le u 365 gtt Val1 9CC Ala tcc Ser atc Ile C ca Pro 270 tat T yr t 99 Tr p ctc Leu 9CC Al a acg Thr 350 att Ile gt 9 Val1 atc Ile atg Met 99C Gly 255 gtg Val tCC Se r

CCC

Pro 99C Gly acc Thr 335 atg Met gCC Ala 9C9 Ala aca Thr 9CC Ala 240 ttc Phe ttc Phe 99g C Gly qaa Glu t9 Trp 320

CCC

Pro aat Asn a tg Met Ctt Le u 9ct Ala 400 tcg Ser 225 9CC Ala acc Th r 99C Gly ttc Phe cca Pro 305 aac Asn atc Ile ctt Leu 99C Gi y caa Gin 385 210 gtt Val1 atc Ile atc Ile Ctg Le u 9CC Ala 290 Ca 9 Gin t ct Se r CaC His 9 C9 Ala ga Gly 370 a Cq Thr 9 ca Ala cac His agt Ser ct C Leu 275 atg Met t9gg T rp 9CC Al a CaC His gga Gly 355 atg Met gtg Val1 acc Thr atg Met ttg Leu 260 aca Thr ctc Leu 9CC Ala Ctc Leu CgC Arg 340 9C t Ala

CCC

Pro ctt Le u gca Ala caa Gin 245 cac His gaC Asp 9CC Al a atg Met gtc Val1 325 acc Thr tcza Ser t tg Le u agt Ser 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1309 1332 aag act CCt tca tgt ttt Ser Cys Phe Phe Ar9 Thr Arg Ser Ile Giu Lys Thr Pro ta9cctagga attcacgcac gac <210> 606 <211> 403 <212> PRT <213> Corynebacterium glutamicum <400> 606 Met His Ala Ser Ser Pro Gin Pro His Pro Gin Ar9 Thr Arg Vai Leu 1 5 10 WO 01/00805 PCT/IB00/00926 Ser Gly Leu Ile Phe Ala Gin Ile Met Val Gly Ala Ser Asn Gly Val 25 Thr Leu Ser Met Gly Ser Leu Leu Ala Ala His Leu Ala Gly Ala Ser 40 Trp Gly Gly Ser Ala Ala Thr Leu Thr Thr Ile Gly Ala Ala Ile Phe 55 Ser Ile Pro Leu Ala Arg Met Val Ser Thr Tyr Asp Arg Arg Thr Ser 70 75 Leu Ser Thr Gly Met Leu Leu Gly Cys Val Gly Ala Leu Leu Ala Ile 90 Leu Gly Ala Gin Phe Gly Leu Phe Pro Val Val Leu Leu Ala Phe Leu 100 105 110 Phe Leu Gly Ser Met Ser Ala Val Asn Leu Gin Ala Arg Phe Ala Ala 115 120 125 Thr Asp Val Ala Ser Glu Glu Thr Arg Gly Arg Asp Leu Ser Ile Val 130 135 140 Val Trp Ser Thr Thr Ile Gly Ala Ile Ala Gly Pro Asn Leu Phe Glu 145 150 155 160 Pro Ser Ala Arg Phe Ser Glu Thr Leu Gly Leu Glu Gin His Ala Gly 165 170 175 Ala Tyr Leu Leu Cys Leu Phe Gly Gin Leu Ile Ala Ile Ala Val Trp 180 185 190 Arg Phe Thr Leu Pro Lys Gly Leu Lys Pro Glu Ala Thr Pro Asn Ala 195 200 205 Pro Thr Glu Lys Lys Arg Leu Thr Pro Lys Ala Leu Gin Ala Ile Thr 210 215 220 Ser Val Ala Thr Ala His Phe Ser Met Val Gly Leu Met Ser Met Ala 225 230 235 240 Ala Ile His Met Gin Gly His Gly Ala Ser Leu Thr Ile Ile Gly Phe 245 250 255 Thr Ile Ser Leu His Val Ala Gly Met Tyr Ala Leu Ser Pro Val Phe 260 265 270 Gly Leu Leu Thr Asp Lys Leu Gly Arg Asn Val Thr Ile Tyr Ser Gly 275 280 285 Phe Ala Met Leu Ala Thr Ser Ala Ala Phe Leu Ile Ile Trp Pro Glu 290 295 300 Pro Gin Trp Ala Met Ile Thr Ser Met Ile Leu Leu Gly Leu Gly Trp 305 310 315 320 Asn Ser Ala Leu Val Gly Ser Ser Thr Leu Leu Val Asp Ala Thr Pro 325 330 335 WO 01/00805 PCTIIBOO/00926 Ile His His Arg Thr Tyr Ala Gin Gly Arg Ser Asp Leu Thr Met Asn 340 345 350 Leu Ala Gly Ala Ser Gly Gly Leu Ile Ala Gly Pro Leu Ile Ala Met 355 360 365 Gly Gly Met Pro Leu Leu Ala Gly Val Val Leu Ala Val Val Ala Leu 370 375 380 Gin Thr Val Leu Ser Phe Arg Thr Arg Ser Ile Glu Lys Thr Pro Ala 385 390 395 400 Ser Cys Phe <210> 607 <211> 760 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (760) <223> FRXA02561 <400> 607 atctgctcaa taacctaacc taaagtccat gcacgcctcg tcgccccaac ctcaccctca gcgcacccgt gttctcagcg qcctgatttt cqcccaaatc atg gtt ggt gca tcc Met Val Gly Ala Ser aat As n gga Gly gct Ala cga Arg ctg Leu 9 ca Ala ttc Phe 99 C Gly gct Al a atc Ile act Thr gcg Al a ttt Phe qcc Ala qtg Val1 t cg Se r ttt Phe tca Ser atc Ile ttg Leu qca Ala atg Met tca Se r ctt Le u 9gC Gi y ca a Gin tcc Ser gCC Ala gga Gly gcc Ala gcc Ala atg Met ttc Phe at g Met ag t Ser agt Ser g cc Al a C 9C Arg ttg Leu qgc Gly t c9 Ser gaa Gi u 110 t tg Le u ggC Gi y ga t Asp gca Ala ct t Leu gca Ala 100 ga c Asp qcg Al a gca Ala cgc Arg cta Le u ttg Leu cg t Arg ctC Le u WO 01/00805 tcg atc gtt gtg tgg tcc acc acc atc Ser Ile Val Val Trp Ser Thr Thr Ile 120 125 tta ttt gaa cca agc gcc cga ttc agc Leu Phe Glu Pro Ser Ala Arg Phe Ser 135 140 cat 9CC 99c gca tac ct9 ctg tgt tta His Ala Gly Ala Tyr Leu Leu Cys Leu 150 155 gca gtc tgg cga ttc acc ctc ccc aaa Ala Val Trp Arg Phe Thr Leu Pro Lys 170 cca aat gca cca aca gaa aag aag cgc Pro Asn Ala Pro Thr Giu Lys Lys Arg 185 190 gcc atc aca tcg gtt gca acc gca cac Ala Ile Thr Ser Val Ala Thr Ala His 200 205 tcc atg 9CC 9CC atc cac atg Ser Met Ala Ala Ile His Met 215 220 <210> 608 <211> 220 <212> PRT <213> Corynebacterium glutarnicum <400> 608 Met Val Gly Ala Ser Asn Gly Val Thr 1 5 Ala Ala His Leu Ala Gly Ala Ser Trp 25 Thr Thr Ile Gly Ala Ala Ile Phe Ser Ser Thr Tyr Asp Arg Ar9 Thr Ser Leu 55 Cys Val Gly Ala Leu Leu Ala Ile Leu 70 Pro Val Val Leu Leu Ala Phe Leu Phe Asn Leu Gin Ala Arg Phe Ala Ala Thr 100 105 Arg Gly Arg Asp Leu Ser Ile Val Val 115 120 Ilie Ala Gly Pro Asn Leu Phe Giu Pro 130 135 9ga Gly Ct C Leu atc Ile ga a Glu gCC Ala 195 99 t Gi y cca Pro gaa Glu 9CC Ala 9CC Al a 180 ctc Le u CtC Le u aat Asn ca a Gln atc Ile 165 acc Thr caa Gin a tg Met T11BO0100926 499 547 595 643 691 739 Leu 10 Gly Ile Ser Gly Leu 90 Asp Trp Ser Se r Gi y Pro Th r Al a 75 Gi y Val1 Ser Ala Met Se r Le u Gly Gin Ser Ala Thr Arg 140 Ser Ala Arg Leu Gly Ser Giu 110 Ile Ser Leu Thr Met Leu Leu Ala Giu Gly Glu Leu Leu Val Gly Ph e Val Thr Al a Th r WO 01/00805 Leu Gly Leu Glu Gin His Ala Gly Ala Tyr Leu 145 150 155 Gin Leu Ile Ala Ile Ala Vai Trp Arg Phe Thr 165 170 Lys Pro Glu Ala Thr Pro Asn Ala Pro Thr Glu 180 185 Pro Lys Ala Leu Gin Ala Ile Thr Ser Vai Ala 195 200 Met Val Gly Leu Met Ser Met Ala Ala Ile His 210 215 <210> 609 <211> 461 <212> DNA <213> Corynebacterium glutarnicum <22 0> <221> COS <222> (438) <223> FRXA02566 PCTJIBOO/00926 Leu Cys Leu Phe Giy 160 Leu Pro Lys Gly Leo 175 Lys Lys Arg Leu Thr 190 Thr Ala His Phe Ser 205 Met 220 <400> 609 atc Ile 1 ctg Leo 9C Al a cag Gin tct Se r cac His gcg Al a qga Gly acq ag t Se r ctc Leu atg Met t gg Trp gcc Ala cac His gga Gly a tq Met gtg cac His gac Asp gcC Al a atg Met gtc ValI acc Thr tca Ser 100 t tg Le u agt gtc gcc gga atg tac gca ctc tca cca Val Ala Gly Met Tyr Ala Leu Ser Pro 5 gtg ttc ggc Val Phe Gly aaa Ly s aca Th r atc Ile 99 t Gly tac T yr ggc Gly ttg Leu ttc ct C Leo tcC Se r aca Thr tct Ser 70 gjc c Al a 9g9 Gly gca Ala ag a 9gC Gly gca Ala tcC Se r 55 t ca Se r C ag Gln t tg Leo Gly a cc acc Th r atc Ile ct t Leo qtc Val1 75 ga C Asp CCg Pro gca Ala gaa atc Ile att Ile 999 Gly ga C Asp cta Leo tta Leu gt t ValI aag tat Tyr t gg Trp ctc Leo

C

Ala a cg Th r att Ile gtg Val 125 act ttc Phe cca Pro aac Asn atc Ile ctt Leu Gly caa Gin t ca 48 96 144 192 240 288 336 384 432 WO 01/00805 PCTIBOOIOO926 Thr Val Leu Ser Phe Arg Thr Arg Ser Ile Giu Lys Thr Pro Ala Ser 130 135 140 tgt tit tagcctagga attcacgcac gac 461 Cys Phe 145 <210> 610 <211> 146 <212> PRT <213> Corynebacteriun giutamicum <400> 610 Ilie Ser Leu His Val Ala Gly Met Tyr Ala Leu Ser Pro Val Phe Gly 1 5 10 Leu Leu Thr Asp Lys Leu Gly Arg Asn Val Thr Ile Tyr Ser Gly Phe 25 Ala Met Leu Ala Thr Ser Ala Ala Phe Leu Ile Ile Trp Pro Glu Pro 40 Gin Trp Ala Met Ilie Thr Ser Met Ile Leu Leu Gly Leu Gly Trp Asn 55 Ser Ala Leu Vai Gly Ser Ser Thr Leu Leu Val Asp Ala Thr Pro Ile 70 75 His His Arg Thr Tyr Ala Gin Gly Arg Ser Asp Leu Thr Met Asn Leu 90 Ala Gly Ala Ser Gly Gly Leu Ile Ala Gly Pro Leu Ile Ala Met Gly 100 105 110 Gly Met Pro Leu Leu Ala Gly Val Val Leu Ala Val Val Ala Leu Gln 115 120 125 Thr Val Leu Ser Phe Arg Thr Arg Ser Ile Giu Lys Thr Pro Ala Ser 130 135 140 Cys Phe 145 <210> 611 <211> 1527 <212> DNA <213> Corynebacteriun glutamicun <220> <221> GUS <222> (1504) <223> RXA00051 <400> 611 cagtaaaagt gcaccgacac taagaatcct tatccttcag agtaagqtga tctqcgtcaa aaaatgtccg ctattcaccc gaaacgaggt cagcatctcg atg aat gcc tcc cct 115 Met Asn Ala Ser Pro 1 9CC Ala atc Ile Gly ggt Gly gtq Val ctt Le u a tg Met aac Asn ctt Le u gag Glu 150 atg Met gct Al a

CCC

Pro gca Ala ga t Asp 230 WO 01/00805 cca acc cg Pro Thr Ar gog ctg gg Ala Leu Gl 2 gca atC ca Ala Ile Gi ggc 9CC gt Gly Ala Va cat cac cc His His Pr ggc gga tg Gly Gly Tr ctc atc gt Leu Ile Va ttc tgg tt Phe Trp Ph~ 120 ctt att gt Leu Ile Va 135 ctc gag tt Leu Giu Ph* atc gtC gg Ile Val Gl gaa gtt gc Glu Val Al 18 aac ggt gt Asn Gly Vi 200 ttc ggt gc Phe Gly G] 215 cct gag ai Pro Glu L~ a .9 t y 5 a n

C

1 a '0 [9 'p

C

1I '5

C

Le -y c' tct Ser tcc Ser gca Ala gt 9 Val1 gtc Val gca Al a tgc Cy s

CCC

Pro 9gt Gly atc Ile Gly 170 99C Gly gaa Giu acc Th r tcc Se r ttt Ph e g9 Ala 9c t Ala tac Tyr cg t Arg 75 99 C Gi y Ct 9 Le u 99C Gly 99C Gly ttc Phe 155 gca Ala gta Val1 qgc Gly gaa Glu atc Ile 235 aaa Lys at c Ile ggt Gly ttc Phe 60 gqt Gly taC Tyr gCt Ala acc Thr gca Al a 140 acc Th r 9CC Ala t~c Ser atg Met at c Ile 220

CCC

Pro gga Gly ggc Gi y cca Pro 45 atg Met tcc Ser atc Ile gac Asp cca Pro 125 aac Asn atc Ile atc Ile aac As n atc Ile 205 atc Ile aag Lys ttg Leu acc Th r 30 tct Ser ctg Leu ttc Phe ac Thr Ctc Leu 110 caa Gin ctc Le u att Ile Ct C Leu ct C Le u 190 9 ca Ala g9t Gi y gct Ala C99 Arg 15 ggc Gly gta Val1 CgC Arg 9 C9 Ala ggC Gly 95 aca Thr t 99 Trp gca Al a aag Lys gca Al a 175 tgg Trp 9CC Ala gtt Val1 gtt Val1 gCt Ala ttg Le u ctc Le u 9 ca Ala 9tc Val 80 tgg Trp gCC Ala act Th r t ca Ser 9tc Val1 160 ttc Phe gag Giu ttc Phe 9 ca Ala aat Asn 240 cga Arg ttc Phe t t9 Le u ctc Le u tac Tyr a tg Met atc Ile t gg Trp gtq Val 145 acc Th r 99 t Giy cac His atc Ile 99C Gi y 225 act Thr cac His tac T yr gtc Val ggC Giy acc T hr ttc Phe 99c Gly gtg Val1 130 cgt Arg g ca Ala ctc Leu 9gC Gly ctt Le u 210 tct Ser gtc Val1 att Ile ggt Gly taC T yr gag Giu Cgc Arg 9c9 Al a atc Ile 115 gta Val1 tgg Trp gtt Val 99C Gly gga Gi y 195 gt t ValI gaa Glu cca Pro cac His t cc Se r ctt Le u atg Met gca Ala ttt Phe 100 tat T yr

C

Ala ttc P he gtc ValI gCC Ala 180 ttc Phe ctc Le u gct Al a gta Val PCTABOOIOO926 ttc 163 Phe gCt 211 Ala ctc 259 Leu gct 307 Ala cac 355 His gag 403 Giu atg 451 Met acc 499 Th r ggt 547 Gi y 9CC 595 Ala 165 aac 643 Asn ttc 691 Phe ttc 739 Phe gaa 787 Glu cqc 835 Arg 245 WO 01/00805 WO 0100805PCTIiBOO/00926 ato Ile ect Pro ga c Asp atc Ile cgc Arg 310 ggc Giy atc Ile cgc Arg gt c Val cct Pro 390 ttc Ph e atg Met ttc Phe geg ct c Leu t gg T rp acc Thr acc Th r 295 qt t Val1 aag Lys atc Ile gtc ValI t gg Trp 375 gcc Ala gga Gly gt c Val ctt Leu atc ctc Le u cct Pro etc Leu 280 gct Ala ctc Leu atc Ile gtc Val ttc Phe 360 ctg Le u ga c Asp caa Gin tgg Trp gtc Val 440 gct ttc tat gtg ggt Phe Tyr Val Gly 250 tcc atc acc ggc Ser Ile Thr Giy 265 ggc gtc aac tgg Giy Vai Asn Trp gca ctg tct gee Ala Leu Ser Ala 300 act ggt ctt gcg Thr Gly Leu Ala 315 gcc aag aac ggc Ala Lys Asn Gly 330 ttg atc gtg gga Leu Ile Val Gly 345 gag atc gte gct Glu Ile Val Ala atg ate etq etc Met Ile Leu Leu 380 gag gtc aag tee Glu Val Lys Ser 395 tac ttc gcg atc Tyr Phe Ala Ilie 410 tac gac aac tac Tyr Asp Asn Tyr 425 ctg atg aca atc Leu Met Thr Ile ccg atc gat tat Pro Ilie Asp Tyr gcc atc ctg Ala Ile Leu gtg ate ctt gcc ctt aat Val Ile Leu Ala Leu Asn 255 260 gaa Glu gct Ala 285 atc Ile aa g Lys gtt Val1 gt a Val1 tee Se r 365 gca Ala ct g Le u eta Le u cac His ctt Leu 445 gaa Glu 270 gct Al a aac As n gaa Gi u c ca Pro ate Ile 350 eta Leu ea g Gin a ag Lys ttt Phe ct g Le u 430 tac Tyr tee Ser gg t Gi y get Ala aae Asn gtc Val1 335 etc Le u gca Al a gtg Val1 ttc Phe ate Ile 415 cca Pro tac Tyr e ca Pro etc Le u ga e Asp etc Le u 320 atg Met aae Asn ace Th r gga Gly ect Pro 400 gee Al a etc Le u gee Al a ttc Phe etc Leu etc Leu 305 gca Ala ace Thr g ca Ala ttc Phe tee Ser 385 gte Val1 tte Phe gee Al a aca Th r gte Val aac As n 290 ttc Phe eca Pro ace Th r gtg Val1 gee Ala 370 ege Arg ccc Pro ace Thr gte Val1 Gly 450 cag Gin 275 gee Ala 9ge Gi y aag Ly s ace Thr ett Leu 355 aca Thr ega Arg ttc Phe tte Phe ggC Gly 435 cga Arg ate ttc Ile Phe gtg gte Vai Val get gge Ala Gly gee atg Ala Met 325 ate atg Ile Met 340 ccc gag Pro Glu gtt tac Val Tyr aac atg Asn Met tac ccc Tyr Pro 405 ggc ate Gly Ile 420 gtt gga Val Gly eca aag Pro Lys 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1504 gaa gag eta gat ceg ega ege gat Glu Glu Leu Asp Pro Arg Arg Asp Ala Ilie Ala 455 4 taatetagae tcgcacgaaa aag 1527 <210> 612 <211> 468 WO 01/00805 <212> PRT <213> Corynebacterium giutarnicum <400> 612 Met Asn Ala Ser Pro Ala Pro Thr Arq PCTIIBOO/00926 1 Arg Phe Leu Leu Tyr Met Ile Trp Val1 145 Th r Gly His Ile Gly 225 Thr Ile Phe His T yr Val1 Gly Thr Phe Gly Val 130 Arq Ala Leu Gly Leu 210 Se r Val1 Le u Val le Gly Tyr Glu Arg Ala Ile 115 Val1 Trp ValI Gly Gly 195 ValI Glu Pro Ala Gin 275 His Se r Le u Met Ala Phe 100 Tyr Ala Phe Val Ala 180 Phe Le u Ala Val Le u 260 Ile 5 Phe Ilie Ala Gly Leu Gly Ala Val 70 His Leu Glu Met Met Asn Thr Leu Gly Giu 150 Ala Met 165 Asn Ala Phe Pro Phe Ala Giu Asp 230 Arg Ile 245 Asn Pro Phe Asp Ala Al a Gly 55 His Gly Leu Phe Leu 135 Leu Ile Giu Asn Phe 215 Pro Le u Trp Thr Le u Ilie 40 Ala His Gly Ile Trp 120 Ile Giu Val1 Val1 Gly 200 Ci y Giu Le u Pro Le u 280 Gly 25 Gin Val Pro Trp Val1 105 Phe Val Phe Gly Al a 185 Val1 Gly Lys Phe Ser 265 Gly Ser 10 Ser Ala Val1 Val1 Ala 90 Cys Pro Gly Ile Gly 170 Gl y Glu Th r Se r Tyr 250 Ile ValI Phe Ala Ala Tyr Arg 75 Gly Leu Gly Gly Phe 155 Ala Val Gly Glu Ile 235 Val1 Th r Asn Lys Ile Gi y Ph e Gly Tyr Ala Th r Ala 140 Th r Ala Se r Met Ile 220 Pro Gly Cly T rp Gly Gly Pro Met Se r Ile Asp Pro 125 Asn Ile Ile Asn Ile 205 Ile Lys Ala Glu Ala 285 Leu Thr Ser Le u Phe Th r Leu 110 Gin Le u Ile Leu Le u 190 Ala Gly Ala Ile Glu 270 Ala Arg Ala Gly Leu Val Leu Arg Ala Ala Val Gly Trp Thr Ala Trp Thr Ala Ser Lys Vai 160 Ala Phe 175 Trp Glu Aia Phe Vai Ala Val Asn 240 Leu Val 255 Ser Pro Gly Leu Leu Asn Ala Val Val Ilie Thr Ala Ala Leu Ser Ala Ile Asn Ala Asp WO 01/00805 Leu Phe Gly Ala Gly Arg Val Leu Thr Gly Leu 305 310 315 Ala Pro Lys Ala Met Gly Lys Ile Ala Lys Asn 325 330 Thr Thr Thr Ile Met Ile Ile Val Leu Ile Val 340 345 Ala Val Leu Pro Giu Arg Val Phe Glu Ile Val 355 360 Phe Ala Thr Val Tyr Val Trp Leu Met Ile Leu 370 375 Ser Arg Arg Asn Met Pro Ala Asp Giu Val Lys 385 390 395 Val Pro Phe Tyr Pro Phe Gly Gin Tyr Phe Ala 405 410 Phe Thr Phe Gly Ile Met Val Trp Tyr Asp Asn 420 425 Ala Val Gly Vai Gly Phe Leu Val Leu Met Thr 435 440 Thr Gly Arg Pro Lys Ala Ile Ala Pro Ilie Asp 450 455 Pro Arg Arg Asp 465 <210> 613 <211> 1578 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1555) <223> RXA01172 <400> 613 taaatttttc tagactaccc agattaagtg agattctttc aacttcggcc acccgacaag agccgttgag cccgacgggt agg ttc gcg ttt agt agc ccg cgg agq ttt cgc Arg Phe Ala Phe Ser Ser Pro Arg Arg Phe Arg 15 ggc ctc gtg gtg gcg ctc gcg ctg att ccg gag Gly Leu Val Val Ala Leu Ala Leu Ile Pro Glu 30 gta tta gct ggc gtg gat cct aag atg ggt ctt Val Leu Ala Gly Val Asp Pro Lys Met Gly Leu Ala Gi y Gly Ala Leu 380 Ser Ile Tyr Ile T yr 460 Lys ValI Val1 Ser 365 Ala Leu Leu His Le u 445 Glu PCT/IBOO/00926 Leu 320 Met Asn Thr Gl y Pro 400 Ala Le u Ala Asp gtgtcttcga caacttcgac gtg ott gcc tog ttt Val Leu Ala Ser Phe 1 acc gag gtt tta qct Thr Glu Val Leu Ala tcg att gcg ttt too Ser Ile Ala Phe Ser ttt gcg tog tgc acc Phe Ala Ser Cys Thr WO 01/00805 WO 0100805PCT/IB00100926 a tq Met 9cg Ala cat His cag Gin C Cq Pro ttt Phe ccg Pro 150 acc Thr atc Ile ga a Glu act Th r ct Le u 230 att Ile atc Ile 9 ca Ala gcg Ala 99c Gly att Ile cg t Arg ttt Phe 135 ci g Le u t cg Ser gtg Val cic Le u ct Le u 215 gtt ValI a cg Th r qca Ala atg Met a ca Thr gtg Val1 g cg Ala tcc Ser 120 g cg Ala ttc Phe q ig Val1 t 99 T rp ccc Pro 200 gag Giu ggg Gly gag Glu aac Asn acc Th r gg c Gly ga a Gi u tg Leu 105 gitt ValI ca 9 Gin Ala att Ile gtt Val 185 tct Se r act Thr t ig Le u gtt Val1 att Ile 265 att Ile gct Al a tat Tyr t cg Ser atg Met ct g Le u ci 9 Leu ccg Pro 170 ttc Phe icc Ser ct a Le u atg Met cac His 250 atc Ile 90cg Ala qgg Val 75 ttc Phe tig Le u cic Leu ccg Pro 9 gt Gi y 155 gci Ala g gja Gly cta Le u aaa Lys gaa Giu 235 t cc Se r acc Th r ct Leu 60 9 cg Ala ctg Leu ct~g Le u gga Gi y cac His 140 att Ile c cg Pro atc Ile cca Pro att Ile 220 t cg Se r aat Asn gcg Ala aca Thr ctg Le u gcc Ala Gi y ttt Phe 125 ct 9 Leu 9gc Gly Ctg Le u aac As n ga a Glu 205 att Ile ctg Leu aaa Lys ttt Phe 99 t Giy Val1 a cg Thr gta Val1 110 gtt Val1 atc Ile atc Ile att Ilie att Ile 190 tc Ph e 999 Gly cig Le u tcC Se r ttg Le u 270 gga Gly att Ile gta Val 95 gcc Al a aat Asn ga t Asp atg Met gitt Val 175 cca Pro ctc Le u ccg Pro acc Th r cgc Arg 255 ggc Gly aaa Lys cg c Arg gca Al a 80 att Ile aag Lys gcg Ala gt i Val1 ci g Le u 160 atc Ile aac As n at Ile tat Tyr cC Al a 240 ga a Gi u Gi y aac As n cct Pro Ccq Pro t tg Leu Ctg Leu ctg Leu ccq Pro 145 tti Phe gig Val1 gt C Val1 ccc Pro gcg Ala 225 a ag Lys qct Ala a ig Met gc g Al a gt g Val1 gcg Al a atg Met gcg Ala 130 tgg Trp tgg Trp

C

Al a tct Ser aac Asn 210 ctt Leu Ci 9 Le u gca Ala 99 t Gi y a tg Met gtt Val ggc Gly C9g Arg 115 tgc Cys atg Met ccg Pro t tg Leu gac Asp 195 gtt ValI gga Gi y gtC Val ggt Gi y 99 C Gly 275 tt Ile C9q Arg at Ile 100 ttc Phe tig Leu gt9 Val aag Lys act Thr 180 cag Gin c9 Pro atg Met gat Asp cag Gin 260 t gc Cys t cc Ser ga t Asp att Ilie ati Ile gig Val1 tat Tyr Ctg Le u 165 gcg Ala ggC Gly ct t Leu t Cg Se r ga t Asp 245 qgc Gly gcg Ala 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 aig aic ggc cag acc aig atc aac gig Met Ile Gly Gin Thr Met Ile Asn Val tca gga gcc cgc act Ser Gly 290 Ala Arg Thr WO 01/00805 WO 0100805PCT/EBOO/00926 cg C Arg ttg Le u 310 gtc Val1 ccg Pro ata Ile atc Ile cac His 390 acc Thr tcc Se r gcc Ala atc Ile ga c Asp 470 tcc Ser 9gc Gly att Ile acc Thr atc Ile 360 gga Gly gtg Val1 aag Lys gac Asp gaa Giu 440 aaa Lys ccc Pro a cc Thr gat Asp atc Ilie ctc Leu 345 atc Ile qtg Val1 tct Ser 9gc Gly tac T yr 425 atc le tac Tyr agc Se r ttc Phe gtc Val1 gtc Val1 330 aaa Lys gcg Ala Ct C Le u gtg Val1 ca g Gin 410 tcc Ser tgg Trp g cc Ala cg C Ar g ttg Le u gtg Val1 315 tcc Se r ttc Phe acc Thr acc Thr gag Glu 395 cta Le u gac Asp qac Asp g cg Ala ga t Asp 475 ttc Phe ccc Pro gcc Ala 335 agt Se r gga Gly atg Met ga c Asp t cc Ser 415 ca g Gln qt a Val agc Ser cgc Arg ctg Le u atg Met 320 ga c Asp gaa Glu aac Asn ttt Phe aac As n 400 t cg Se r atc Ile gcc Ala gtg ValI cta Le u ctg Le u 305 gcg Ala tgg Trp a cg Th r ct g Leu gc t Ala 385 aac Asn aac Asn atc Ile acq Thr gag Glu 465 tca Ser ctc Le u g cg Ala cat His atc Ile g cg Ala 370 cgc Arg atc Ile ga t Asp atc Ile ctg Leu 450 atc Ile Gly ctg Le u ct C Le u t cg Ser gt c Val1 355 atc Ile cgc Arg agc Se r atg Met ga t Asp 435 ga c Asp atc Ile aag Lys gtg Val1 gtg Val1 ctg Le u 340 atg Met gg C Gi y gtg Val1 acc Th r gt g Val1 420 ctc Le u agc Se r ggg Gly ct 9 Le u gt t ValI gca Ala 325 aac As n ttt Phe gt c Val gcc Ala tat Tyr 405 tac Tyr acc Thr atc Ile Ct C Leu g gc Gly 485 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1578 taaaaatttg Cttatcgacg cgc <210> 614 <211> 485 <212> PRT <213> Corynebacterium giutarnicum <400> 614 Val Leu Ala Ser Phe Arg Phe Ala Phe Ser Ser Pro Arg Arg Phe Arg 1 5 10 wo 01/00805 WO 0100805PCTJIBOO/00926 Thr Se r Phe Pro Pro Leu Leu Leu Pro 145 Phe Val1 Val Pro Ala 225 Ly s Ala Met Ser Leu 305 Ala Val1 Ala Se r Met Val1 Gly Arg 115 Cys Met Pro Leu Asp 195 Val1 Gly Val1 Gly Gly 275 Ala Leu Leu Leu Phe Cys Ile Arg I le 100 Phe Le u Val1 Lys Th r 180 Gln Pro Met Asp Gln 260 Cys Arq Val1 Val1 Ala Gly Leu Val Val Ala Le~u Ala Leu Ile Pro Glu 25 Val Met Ala 70 His Gln Pro Phe Pro 150 Thr Ile Glu Th r Le u 230 I le Ile Met Arg Le u 310 Val1 Le u Ala Al a Gly Ile A rgc Phe 135 Le u Ser Val Le u Le u 215 Val1 Thr Ala Ile Le u 295 Leu Met Ala 40 Met Th r Val Ala Se r 120 Ala Ph e Val Trp Pro 200 Glu Gly Glu As n Gly 280 Se r Gly Ile Gi y Thr Gly Glu Le u 105 Val1 Gin Ala Ile Val1 185 Ser Thr Le u ValI Ile 265 Gin Th r Asp Ile Val Ile Al a Tyr Se r Met Leu Leu Pro 170 Phe Ser Leu Met His 250 Ile Thr Phe Val1 Val1 330 Lys Asp Ala ValI 75 Phe Leu Leu Pro Gi y 155 Ala Gly Le u Lys Glu 235 Se r Th r Met Le u Val1 315 Se r Phe Pro Leu Ala Le u Le u Gly His 140 Ile Pro Ile Pro Ile 220 Se r Asn Ala Ile Ala 300 Gly Ile Met Lys Thr Leu Ala Gly P he 125 Leu Gi y Le u Asn Giu 205 Ile Le u Lys Phe Asn 285 Gly Ly s Asp Pro Met Gly Val1 Th r ValI 110 Val Ile Ile Ile Ile 190 Phe Gly Le u Ser Leu 270 Val Gly Ile Thr Le u Gly Leu Gly Arg Ile Ala Val Ile Ala Lys Asn Ala Asp Val Met Leu 160 Val Ile 175 Pro Asn Leu Ile Pro Tyr Thr Ala 240 Arg Glu 255 Gly Gly Lys Asn Phe Leu Pro Met 320 Ala Asp 335 Ser Glu Trp His Ser Leu Asn Pro Arg Thr Leu Thr Le u Al a 385 As n As n Ile Th r Glu 465 Ser WO 01/00805 340 Ile Val Met 355 Ala Ile Gly 370 Arg Arg Val Ile Ser Thr Asp Met Val 420 Ile Asp Leu 435 Leu Asp Ser 450 Ile Ilie Gly Gly Lys Leu Phe Val1 Ala T yr 405 T yr Thr Ile Le u Giy 485 PCT/BOOIOO926 Ile Ile His 390 Th r Ser Ala Ile Asp 470 Thr Leu 375 Le u Val Ph e Aia His 455 Giy Le u Ala 380 Arq Phe Glu Ala Arq 4 Arg Val 365 Met Thr Trp Aila Se r 445 Gly Le u 350 Thr Val1 Th r Ala Giu 430 Thr Lys Gi u <210> 615 <211> 1818 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(1795) <223> RXA02128 <400> 615 tattagcaaa acttcttaaa gagccttttt gtgcctttat gagtattaag tgggcgcgct ttgtaccggg gataaccgcg cga Arg tac Tyr gc t Ala ttc Phe aag L ys caa Gin ct g Leu tct Se r 9gt Gly gtc Val tg Trp cga Arg ggt Giy gt c Vai

CCC

Pro gg t Giy ccgagtatct ttaaaaqcat atg cgg gqc tat caa Met Arg Gly Tyr Gin 1 ata acc gtg gcc gcg Ile Thr Val Ala Ala att gcg ggg ctg cca Ile Ala Gly Leu Pro atg qcg ctg tac ttt Met Ala Leu Tyr Phe cct gaa tca acc acc Pro Giu Ser Thr Thr WO 01/00805 PCT1B00100926 60 gct ctg atg acg gct gca ggt qtg gga gct tta gtc ggg gca gct ggc 355 Ala Leu Met Thr Ala Ala Gly Val Gly Ala Leu Val Gly Ala Ala Gly 75 80 ggg cct gaa cga tac gca gaa gta gcg gca cta ttg gct att gca gtg 403 Gly Pro Glu Arg Tyr Ala Glu Val Ala Ala Leo Leu Ala Ile Ala Val 95 100 ggc att gta tgc gct gtt ggt ttt att ggc cga ttg gga ttt ctt acc 451 Gly Ile Val Cys Ala Val Gly Phe Ile Gly Arg Leu Gly Phe Leu Thr 105 110 115 agg ctg ttg tct cga ccg gtg ctc gtt gga tat ttg atc ggt att gca 499 Arg Leo Leu Ser Arg Pro Vai Leu Val Gly Tyr Leu Ile Giy Ile Ala 120 125 130 gtc ttg atg atc gtc agt cag ctg tcc aaa gtc acc cag gtg aat gtg 547 Val Leo Met Ile Val Ser Gin Leu Ser Lys Val Thr Gin Vai Asn Vai 135 140 145 gag agc ggt cag acg tgg cag gaa ata ata tcg ttt atc aaa gta gct 595 Glu Ser Giy Gin Thr Trp Gin Glu Ile Ile Ser Phe Ile Lys Vai Ala 150 155 160 165 ggc cag gca cat att cct aca gtg att ttg gca gtc gtg gtg ttg agc 643 Gly Gin Aia His Ile Pro Thr Val Ile Leo Ala Val Val Val Leo Ser 170 175 180 ttg ctg tat ctg gca aat tgg ttg acg cct aaa ttt ccc agc aca ctc 691 Leo Leo Tyr Leo Ala Asn Trp Leu Thr Pro Lys Phe Pro Ser Thr Leo 185 190 195 atg gtt ctt ctg ctt tcg gca gcc gcg gtg gg ttt ttt cat ctg gat 739 Met Val Leo Leo Leo Ser Ala Ala Ala Val Gly Phe Phe His Leo Asp 200 205 210 agg ttt ggt ctt gag gtc att ggt gag gtg ccc cgt ggc ctg cct caa 787 Arg Phe Gly Leo Glu Val Ile Gly Glu Vai Pro Arg Gly Leo Pro Gin 215 220 225 cca agt att ccc tcg att ggc gat cta gag atc tgg tcg ttg ttg ccc 835 Pro Ser Ile Pro Ser Ile Gly Asp Leo Glu Ile Trp Ser Leo Leo Pro 230 235 240 245 tat gcc gtg ggt att gcc atc gtt ggt ttt tca gac aat gtg ttg act 883 Tyr Ala Vai Gly Ile Ala Ile Val Giy Phe Ser Asp Asn Val Leo Thr 250 255 260 gct cgt gca ttc gcg tcg gga aaa gat gag gtg att gat tcc aac cag 931 Ala Arg Ala Phe Ala Ser Gly Lys Asp Glu Val Ile Asp Ser Asn.Gln 265 270 275 gag ctg ctc gca ctg gga acc gca aac ctg gcg aat ggg ttc ttc cag 979 Glu Leo Leu Ala Leo Gly Thr Ala Asn Leo Ala Asn Gly Phe Phe Gin 280 285 290 gga ttt cct gtg tca tcg agt ggc tcc cqa act gtt ctt gga gac acg 1027 Gly Phe Pro Val Ser Ser Ser Gly Ser Arg Thr Val Leo Gly Asp Thr 295 300 305 WO 01/00805 WO 0100805PCT/EBOO/00926 gca Al a 310 atc Ile g cg Ala aic Ie aic Ile ggg Gi y 390 at Ilie gga Gly ci C Le u gat Asp gtg Val1 470 a cg Thr ggt Gly Cc Le u gg t Gly atg Met g ca Ala gca Ala aca Thr 375 atc Ilie a cc Thr atg Met gig Val1 tt Phe 455 cat His gcc Ala atc Ile gag Glu cg c Arg ctg Leu ggc Gi y 345 atc Ile gct Ala gtt Val1 cci Pro agc Ser 425 ttt Phe aaa Lys ttt Phe gat Asp ttt Phe 505 gca Ala act cag gig cat ica cii gic Thr Gin Val His Ser Leu Val 315 320 gig gig Val Val gcg cig gig Ala Leu Val tg Le u 330 gcc Ala aaa Lys act Thr gcg Ala tat Tyr 410 iig Le u aga Arg cg t Arg tia Le u g cc Ala 490 gcg Ala ggt Gly ttt Phe ta Leu agg Arg g ci Ala gi i Val1 395 gcc Ala gag G lu tac T yr gcc Ala cig Le u 475 atg Met aig Met tic Phe gci Ala gitt Val1 aic Ile gca Ala 380 a cg Thr gat Asp gat Asp ga t Asp aic Ile 460 aat Asn gaa Glu gcc Ala ati Ile gig ValI 335 gca Ala tic Phe gig Val1 atc Ile gga Gly 415 gag Glu.

cig Leu gitt Val1 gcg Ala cg C Arg 495 aag Lys gig Val1 cic Le u gca Ala cg c Ar g gct Ala tig Le u 400 tat Tyr t cg Se r iii Phe gati Asp aai As n 480 aaa Lys ca a Gin ggg Gi y gag Glu a cg Thr a ag Lys ict Ser 385 ga t Asp a cg Thr a ca Thr tic Phe gaa Glu 465 a cg Thr acc Thr gat Asp gag Glu ict Ser ca g Gin agc Ser 370 ggc Gly cic Leu ccg Pro gca Ala gcc Ala 450 gca Ala gaa Glu cig Le u cia Le u gag Giu 530 tc Phe t ig Le u 355 gag Glu gig ValI aic Ile Gly gi C ValI 435 aac Asn act Th r gt Val1 gag Glu cgc Arg 515 tac Tyr c ca Pro 340 at Ile tig Le u cic Le u a ga Arg aig Met 420 gaa Giu gct Ala caa Gin gat Asp gaa GI u 500 cga Arg at Ile 325 ga t Asp ga t Asp gi c Val1 g cg Ala cgt Arg 405 gct Al a ggg Gly ga t Asp ccc Pro ci c Le u 485 cgg Arg agc Se r tc Phe 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 gcc aca cic ccc act gca gic aag Ala Thr Leu Pro Thr Ala Val Lys ggg tat icc gig gag iii cgc gat Gly Tyr Ser Val Glu Phe Arg Asp 545 WO 01/00805 PCTJIBOO/00926 cgt ttt gga aac tat cca gaa ggc qtt ccg aaa gaa att ttg gaa ctt 1795 Arg Phe Gly Asn Tyr Pro Glu Gly Val Pro Lys Giu Ile Leu Glu Leu 550 555 560 565 taagctqcct ggttggcgga ctt 1818 <210> 616 <211> 565 <212> PRT <213> Corynebacterium giutainicum <400> 616 Met Arg Gly Tyr Gin Arg Ser Trp Leu Lys Gly Asp Val Ile Ala Gly 1 5 10 Ile Thr Val Ala Ala Tyr Leu Val Pro Gin Val Met Ala Tyr Ala Val 25 Ile Ala Gly Leu Pro Ala Val Val Gly Leu Trp Gly Val Leu Ala Pro 40 Met Ala Leu Tyr Phe Phe Leu Gly Thr Ser Arg Asn Leu Ser Val Gly 55 Pro Giu Ser Thr Thr Ala Leu Met Thr Ala Ala Gly Val Gly Ala Leu 70 75 Val Giy Ala Ala Gly Gly Pro Glu Arg Tyr Ala Giu Val Ala Ala Leu 90 Leu Ala Ile Ala Val Gly Ile Val Cys Ala Val Gly Phe Ile Gly Arg 100 105 110 Leu Gly Phe Leu Thr Arg Leu Leu Ser Arg Pro Val Leu Val Gly Tyr 115 120 125 Leu Ile Gly Ile Ala Val Leu Met Ile Val Ser Gin Leu Ser Lys Val 130 135 140 Thr Gin Val Asn Val Giu Ser Gly Gin Thr Trp Gin Glu Ile Ile Ser 145 150 155 160 Phe Ile Lys Val Ala Gly Gin Ala [His Ile Pro Thr Val Ile Leu Ala 165 170 175 Val Val Val Leu Ser Leu Leu Tyr Leu Ala Asn Trp Leu Thr Pro Lys 180 185 190 Phe Pro Ser Thr Leu Met Val Leu Leu Leu Ser Ala Ala Ala Val Gly 195 200 205 Phe Phe His Leu Asp Arg Phe Gly Leu Glu Val Ile Gly Glu Val Pro 210 215 220 Arg Gly Leu Pro Gin Pro Ser Ile Pro Ser Ile Gly Asp Leu Giu Ile 225 230 235 240 Trp Ser Leu Leu Pro Tyr Ala Val Gly Ile Ala Ile Val Gly Phe Ser 245 250 255 WO 01/00805 WO 0100805PCT/EBOO/00926 Asp Asn Val Leu Thr Ala Arg Ala Phe Ala Ser Giy Lys Asp Giu Val Ile As n Val1 305 Val1 Giu Thr Lys Se r 385 Asp Thr Thr Ph e Glu 465 Thr Thr Asp Glu ValI 545 Giu Asp Gly 290 Le u ValI Ser Gin Ser 370 Gly Le u Pro Ala Ala 450 Ala Glu Le u Le u Giu 530 Giu Ile Ser 275 Phe Gly Ala Phe Le u 355 Giu Val1 Ile Gly Val1 435 As n Th r Val1 Giu Arg 515 T yr Phe Le u 260 As n Phe Asp Le u Pro 340 Ile Leu Le u Arg Met 420 Glu Ala Gin Asp Giu 500 Arg Ile Arg Glu Gin Giu Gin Gly Thr Ala 310 Val Ilie 325 Asp Ala Asp Ilie Val Ilie Ala Gly 390 Arg Ile 405 Ala Gly Gly Leu Asp Asp Pro Val 470 Leu Thr 485 Arg Gly Ser Leu Phe Ala Asp Arg 550 Leu 565 Le u Phe 295 Gly Met Ala Ala Thr 375 Ilie Thr Met Val1 Phe 455 His Ala Ile Giu Th r Le u 280 Pro Ala ValI Le u Giu 360 Ala Gly Arg His Val 440 Se r Trp ValI Arg Pro 520 Le u 265 Ala Val Arg Leu Gly 345 Ile Ala Val1 Pro Ser 425 Phe Lys Ph e Asp Phe 505 Al a Pro Le u Se r Thr Le u 330 Ala Lys Thr Ala T yr 410 Leu Ar g Arg Le u Ala 490 Ala Gly Th r Gly Se r Gin 315 Phe Le u Arg Ala Val1 395 Ala Giu T yr Ala Le u 475 Met Met Phe Ala Thr Se r 300 Val1 Ala Val1 Ile Ala 380 Thr Asp Asp Asp Ile 460 As n Giu Ala Ile Val1 540 Ala 285 Gly His Gly Ile Ala 365 Ser Le u Val1 T yr Ser 445 Giu Ala Ala Arg Giu 525 Lys 270 As n Se r Se r Pro Tyr 350 Arg ValI Se r Le u Pro 430 Pro Ala Giu Le u Val1 510 Se r Gly Le u Arg Le u Val1 335 Al a Phe Val Ile G ly 415 Giu Le u Val Ala Arg 495 Lys Val1 Tyr Ala Thr Val1 320 Le u Ala Arg Ala Le u 400 Tyr Ser Phe Asp As n 480 Lys Gin Gly Ser Lys 560 Phe Gly Asn Tyr Pro Giu Gly Val Pro 555 WO 01/00805 <210> 617 <211> 1734 <212> DNA <213> Corynebacterium giutarnicum <220> <221> CDS <222> (1711) <223> RXA02634 <400> 617 caacgcgaag gcttagttgt tiattaccgg ctcacciacc PCTIBOOIOO926 cggaggtcgc agactictt aaggtgagcc gctcactati aaagaggatg gctggcgaag atg cgt gac ctt tta Met Arg Asp Leu Leu ccc Pro ati Ile gcc Ala gcg Ala cagc Gin gtc Val1 gga G iy agt Ser gig Vali cat His 150 t ci Se r gci Ala ttt Phe ati Ile gt i Vali gcc Aia gt i Vali itc Phe att Ile 135 ttig Le u a ga Arg gct Aia gg t Giy ati Ile ica Se r caa Gin aic Ile at Ile 120 ati Ilie ccc Pro gac Asp gg C Gi y gig Vali gci Aia gga Giy tat Tyr gi c Vali 105 ccg Pro tic Phe a cc Thr ga c Asp gtc ValI agt Se r ggc Giy cca Pro ggt Giy ct t Leu i gg Trp ct g Le u aat Asn tat Tyr acc Thr ict Se r ctg Le u aca Thr 75 gic Vali gic Vai ccg Pro caa Gin gcg Ala 155 cag cta Gin Leu gtg ggc Vai Giy gga gtc Gly Val 45 gtt gca Val Ala 60 gga gcq Gly Aia ggi gcg Giy Aia gcc gga Aia Giy gtc atc Val Ilie 125 cag gig Gin Vai 140 cic cic Leu Leu ctc Leu at Ile 30 gga Gly gca Aia aig Met gt t Val1 gig ValI 110 gaa Giu cca Pro gct Ala cgc Arg 15 gt Val gcc Ala aic Ile gig Vali g ca Aia 95 ct Le u gga Giy gc t Aia gcg Ala tic Phe gcc Ala gag Giu tic Ph e gt t Vali 80 tia Le u cg a Arg iii Phe g cg Ala ati Ile 160 1 icc Se r cic Le u gca Ala gg C Gly gt Val cic Le u tia Leu a cg Thr iii Phe 145 cac His t gg Trp ccc Pro gga Gly ggt Gly ctc Le u agc Ser ggc Giy gca Ala 130 gg i Giy act Thr a ag Lys cic Leu ita Le u icc Se r gc t Ala ci g Le u cg a Arg 115 gg c Gly tat Tyr gi i ValI at g Met gci Ala gig Val aai As n cca Pro aig Met 100 a cg Thr aic Ile i cg Ser ica Se r ga c Asp cic Leu act Th r gig Vali aic Ile gct Ala gig Vai ggt Gly ggc Gly cac His 165 115 163 211 259 307 355 403 451 499 547 595 gcc acg aag gac gca ati cia cci cic tia ait ati aic Aia Thr Lys Asp Ala Ile Leu Pro Leu Leu Ile Ile Ile gic acc gct Val Thr Ala 180 WO 01/00805 PCT[IBOO/00926 g9 Ala ttc Phe ccc Pro ctg Le u 230 ttt Phe gtg Val1 ttg Le u a tg Met ggC Gly 310 ggc Gly gca Ala ga a Giu ttt Phe gtc ValI 390 ag C Ser ctt atc atg att gtg ttg gga aag atc gcc cca aag ctc cct gcc agc Ile att Ile gt t Val1 215 cct Pro gct Al a gca Al a gt C Val1 ccg Pro 295 ggg Gi y gt t Va I ctg Le u 9t 9 Val1 gtC Val1 375 ggc Gly atg Met aac Met 9CC Ala 200 gag Giu ga t Asp gt C Val gcc Ala g9t Gly 280 gca Al a c9 t Arg gtg Val1 t Cg Ser atc Ile 360 atc Ile atc Ile aac Asn ga c Ilie 185 att Ilie ttg Le u gtg Val1 Ala tcC Ser 265 cag Gin act Th r acc Thr tat T yr 99 C Gly 345 tcg Se r a cc Th r ggC Gly

C

Al a ga a Val1 ttg Le u at c Ile aac Asn 9 C9 Ala 250 atg Met qgg Gly gg c Gly cgt Arg gt 9 Val 330 gtg Val C9 C Arg 9 C9 Ala att Ile ggC Gly 410 a ag Le u gtg Val ggt Gly Ct 9 Leu 235 ctc Le u

C

Ala ttq Le u gca Ala ata Ile 315 9 C9 Ala ctc Le u gtc ValI atc Ile 9CC Ala 395 9tC ValI atc Gly gtg Val1 gag Glu 220 gag Glu gct Ala ga t Asp gcg Ala atc Ile 300 gcg Ala 9 C9 Ala a tg Met atg Met gtC Val1 380 gt C Val1 t tC Phe 9C Lys t CC Ser 205 ct C Le u atg Met gc t Al a a cg Th r t cg Se r 285 gcg Al a t cg Ser aat As n gtC Val Cc Arg 365 acc T hr g cq Ala C9 Arg ctt Ilie 190 att Ilie

CCC

Pro ttC Phe att Ile ggt Gly 270 ata Ile cgt A rg att Ile atc Ile acc Th r 350 t CC S er atc Ile a cg Th r gaa Giu ttc Al a 99C Gly aat Asn a Cg Thr gag Glu 255 CC t Pro agc Ser acg Th r att Ile 9t t ValI 335 gcg Ala act Th r agc Se r ttC Phe acc Thr 415 Cg t Pro gtg Val1 tcc Se r agt Se r 240 tct Se r tat Tyr gct Ala gcg Ala ca c His 320 gcg Ala agc Ser cgC Arg gtc Val1 ttc Phe 400 t tg Le u atc Lys 9 ct Ala ctt Le u 225 ttg Le u ctc Leu aat Asn 99C Gly gt C Val1 305 gCg Ala gtC Val1 Cg C Ar g tct Se r ga C Asp 385 atg Met

CCC

Pro gag Le u Ct 9 Le u 210 cct Pro ct 9 Le u Ctt Le u gc t Ala ttt Ph e 290 aat As n ctt Le u att Ile atg Met ga t Asp 370 ctc Le u ct g Le u 9 aa Glu 99C Pro Ala 195 ctc aag Leu Lys 9CC cct Ala Pro 999 cca Gly Pro tcC gct Ser Ala 260 gac cgc Asp Arg 275 ttt ggC Phe Gly gtg cgc Val Arg gtg ctg Val Leu ccg ctc Pro Leu 340 gtg tCC Val Ser 355 gcg atC Ala Ile gtc att Val Ile cga CgC Arg Arg CCg 9CC Pro. Ala 420 gca ttg Ser ctt Leu cac His gct Ala 245 C9 t Ar g gaa Gl u Gly tcc Se r ctc Leu 325 9CC Al a att Ile gtt ValI 9 Cg Ala atg Met 405 a C9 Thr ttc 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 WO 01/00805 WO 0100805PCTI1BOO/00926 Leu Asn Asp Glu Lys Ile Gly Leu P 4254 ttt ggc gca gct gaa cgt ctt tcg C Phe Gly Ala Ala Glu Arg Leu Ser G 440 445 ctc gaa gtg gtg atc ctg cgc ctg t Leu Glu Val ValIle Leu Arg Leu S 455 460 acc ggc gcc cac cag ctc acc gag c Thr Gly Ala His Gin Leu Thr Giu I 470 475 aat gtc act gta tta att aag ggc Asn Val Thr Val Leu Ilie Lys Gly\ 490 ctc ggc gtc ctc gga gcg atc agat Leu Gly Val Leu Gly Ala Ile Arg 505 ttc gat gac ctt gcc cca gcg gtt The Asp Asp Leu Ala Pro Ala Val 520 525 atc gac aac agt taaggcttcc ggggcc Ilie Asp Asn Ser 535 <210> 618 <211> 537 <212> PRT <213> Corynebacterium giutamicum <400> 618 Met Arg Asp Leu Leu Pro Ser Arg 1 5 Ser Trp Lys Met Asp Ile Ala Ala Leu Pro Leu Ala Leu Ala Phe Gly 40 Ala Gly Leu Val Thr Ala Ile Ile Gly Gly Ser Asn Val Gin Val Ser 70 Val Leu Ala Pro Ile Val Ala Gin Leu Ser Leu Met Ala Gly Val Ile 100 Leu Gly Arg Thr Val Ser The Ile 115 120 ~he Arg Ile 130 :ag caa atc 1n Gin Ile :ca cac atc ~er His Ile ~tt gtc aat ~eu Val Asn 480 ~tc cgg aaa l Arg Lys 495 :ca ctg cgg 3er Leu Arg 510 jaa cat gcc 3lu His Ala gatct aaa Glu ctt Le u caa Gin 465 gcg Ala gaa Giu cat His cqa Arg Gly ga t Asp 450 a tg Met ct g Le u cac His gag Giu aaa Lys 530 Phe ga c Asp gcc Ala aaa Lys 485 gt t Val1 cic Le u aag Lys 1459 1507 1555 1603 1651 1699 1734 Asp 10 Val1 Ser Gly Pro Gly 90 Le u Trp Tyr Th r Se r Le u Th r 75 Val Val Pro Le u Gly Val1 Ala Al a Ala Gly Ile 125 Le u Ile Gly Al a Met Val Val 110 Glu Arq Phe Val Ala Ala Glu Ile Phe Val Val Ala Leu Leu Arg Gly Phe WO 01/00805 PCT/IB00/00926 Thr Ala Gly Ile Gly Val Ile Ile Phe Leu Gin Gin Val Pro Ala Ala 130 135 140 Phe Gly Tyr Ser Gly His Leu Pro Thr Asn Ala Leu Leu Ala Ala Ile 145 150 155 160 His Thr Val Ser His Ala Thr Lys Asp Ala Ile Leu Pro Leu Leu Ile 165 170 175 Ile Ile Val Thr Ala Ala Ile Met Ile Val Leu Gly Lys Ile Ala Pro 180 185 190 Lys Leu Pro Ala Ser Phe Ile Ala Ile Leu Val Val Ser Ile Gly Val 195 200 205 Ala Leu Leu Lys Leu Pro Val Glu Leu Ile Gly Glu Leu Pro Asn Ser 210 215 220 Leu Pro Ala Pro His Leu Pro Asp Val Asn Leu Glu Met Phe Thr Ser 225 230 235 240 Leu Leu Gly Pro Ala Phe Ala Val Ala Ala Leu Ala Ala Ile Glu Ser 245 250 255 Leu Leu Ser Ala Arg Val Ala Ala Ser Met Ala Asp Thr Gly Pro Tyr 260 265 270 Asn Ala Asp Arg Glu Leu Val Gly Gin Gly Leu Ala Ser Ile Ser Ala 275 280 285 Gly Phe Phe Gly Gly Met Pro Ala Thr Gly Ala Ile Ala Arg Thr Ala 290 295 300 Val Asn Val Arg Ser Gly Gly Arg Thr Arg Ile Ala Ser Ile Ile His 305 310 315 320 Ala Leu Val Leu Leu Gly Val Val Tyr Val Ala Ala Asn Ile Val Ala 325 330 335 Val Ile Pro Leu Ala Ala Leu Ser Gly Val Leu Met Val Thr Ala Ser 340 345 350 Arg Met Val Ser Ile Glu Val Ile Ser Arg Val Met Arg Ser Thr Arg 355 360 365 Ser Asp Ala Ile Val Phe Val Ile Thr Ala Ile Val Thr Ile Ser Val 370 375 380 Asp Leu Val Ile Ala Val Gly Ile Gly Ile Ala Val Ala Thr Phe Phe 385 390 395 400 Met Leu Arg Arg Met Ser Met Asn Ala Gly Val Phe Arg Glu Thr Leu 405 410 415 Pro Glu Pro Ala Thr Leu Asn Asp Glu Lys Ile Gly Leu Phe Arg Ile 420 425 430 Glu Gly Ala Leu Phe Phe Gly Ala Ala Glu Arg Leu Ser Gin Gin Ile 435 440 445 WO 01/00805 Leu Asp Tyr Glu Asp Leu Glu Val Vai Ile Leu 450 455 Gin Met Ile Asp Ala Thr Gly Ala His Gin Leu 465 470 475 Ala Leu Glu Arg Lys Asn Val Thr Val Leu Ile 485 490 Glu His Ilie His Vai Leu Gly Val Leu Gly Ala 500 505 His Giu Asn His Leu Phe Asp Asp Leu Ala Pro 515 520 Arg Lys His Val Lys Ile Asp Asn Ser 530 535 <210> 619 <211> 1410 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1387) <223> RXN02233 <400> 619 atcecaccc aattggeccca agagctgcag agttacgttg ctgtcgattt cccaagagcc eeettgggag tcgataatta PCT/IBOO/00926 Arg Leu Ser His Ile 4 Thr Giu Leu Vai Asn 480 Lys Gly Val Arg Lys 495 Ile Arg Ser Leu Arg 510 Ala Val Giu His Ala 525 tagaaccac ctctgcctaa aca Th r gca Ala ate Ile ccc Pro ct g Le u tac T yr gte t gg Trp gt t Val1 ggt Gly aca Thr g ga Gly ct 9 Le u cat gga Gi y gtc Val1 atg Met ctc Leu aca Thr ggt Gly ggc tgg Trp gct Al a cag Gin acc Thr atc Ile ag t Se r att acc gtc Thr Val cct aaa Pro Lys cac gtg His Val gga ttt Gly Phe ctg ttc Leu Phe 75 tet ttt Ser Phe ggc gtg cac His gag Glu atc Ile ceg Pro 60 ct g Le u get Ala ca g gga Gly cgc Arq gee Ala 45 gtc ValI ttg Le u ttc Phe ate ga c Asp ctg Leu 30 atg Met aac As n ate Ile att Ile gqt gge Gly 15 age Se r ttC Phe acc T hr ace Th r g ca Ala 95 ggc aaa Lys tgg Trp ggc Gly aca Tb r aga Arg 80 cct Pro att gtg Val1 1 aag Lys ggg Gly gee Ala ct t Le u aac Asn tta Le u ctt etc gtg act tea Leu Val Thr Ser ate Ile eg C Arg a eg Thr tta Le u cga Arg ace Tb r gte gaa Giu aca Thr etc Le u tte Phe eta Le u gea Al a gca ec Pro att Ile etg Le u tct Se r ccc Pro ace Thr 100 gg t gge Gly gga Gly gtt ValI gg t Gly tcg Ser caa Gin etc 115 163 211 259 307 355 403 451 WO 01/00805 Val His Gly Ile Gly Val Gin Ile PCT/IBOOIOO926 Gly Ile Leu Vai Ala Gly Leu 115 gtg ctc Val Leu att gat Ilie Asp 135 atc ggc Ile Giy 150 cca ctg Pro Leu gca ggc Ala Gly atc qqc Ile Gly gca gac Ala Asp 215 aag ccg Lys Pro 230 atc gtg Ile Val atg aca Met Thr gac gga Asp Gly acc acc Thr Thr 295 tcc acc Ser Thr 310 ttc atc Phe Ile 105 gtC gcC Vai Ala 120 gct gtc Ala Val ctg aac Leu Asn gtt gcc Val Ala cgc gga Arg Gly 185 tgg gtt Trp Val 200 acc atc Thr Ile gaa ttC Glu Phe ctc atc Leu Ile ggg gag Gly Glu 265 ttc ggc Phe Gly 280 tac gca Tyr Ala gct gcg Ala Ala ccc aaa Pro Lys att Ile atg Met ctg Le u a cg Thr 170 atg Met ttc Phe cg t Arg ca g Gin gct Ala 250 ga c Asp acc Thr gaa Glu tac T yr ttC Phe 330 gga Gly cca Pro gca Ala 155 g cg Ala att Ile gca Ala gaa Glu ctc Le u 235 gaa Giu ct C Le u acc Thr aac Asn tgg Trp 315 gg t Gly ttt Phe ccc Pro 140 cca Pro a cc Thr gct Ala gct Ala gca Ala 220 tct Ser aac Asn ga c Asp ctc Le u atc Ile 300 gtt Val1 g ca Ala gtg gtg aaa gca gcg ggc Val Val Lys Ala Ala Gly 125 130 gct gtc acc ggt gcg atc Ala Val Thr Gly Ala Ile 145 acc gcg gca gga aac ttc Thr Ala Ala Giy Asn Phe 160 ctc ttt gcc att ttg atc Leu Phe Ala Ile Leu Ile 175 cgc cig ggc att ttg atc Arg Leu Gly Ilie Leu Ile 190 atc acc ggc aac ctc tca Ile Thr Gly Asn Leu Ser 205 210 gca tgg ttc gga ctg cca Ala Trp Phe Gly Leu Pro 225 qcc att ttg gtg aca ctg Ala Ile Leu Val Thr Leu 240 gtc ggc cac gtc aaa qca Val Gly His Val Lys Ala 255 gac ctc gcc ggc gac gca Asp Leu Ala Gly Asp Ala 270 gca ggt gcc ttc ggt gga Ala Gly Ala Phe Gly Gly 285 290 ggc gtc atg gcg gcc acc Gly Vai Met Ala Ala Thr 305 gcc gcg tgc act gcc atc Ala Ala Cys Thr Ala Ile 320 ctg atc ttc acc atc ccc Leu Ile Phe Thr Ile Pro 335 ctt tac ggc cta atc ggt Leu Tyr Gly Leu Ile Gly aaa L ys gt g Val1 tcc Se r gct Al a gg t Giy 195 gaa Giu ca g Gin cca Pro gt c Vali ctt Leu 275 tcc Ser cg C Arg g cc Aia gcc Ala cg c Arg gca Ala agc Ser acc Thr 180 gtg ValI Gly ttc Phe gtc Val tca Se r 260 atc Ile Giy gt a Val ctt Le u ggc Gly 340 gtt Val ctc Le u caa Gin 165 gtt Val gtg Val g ca Ala ca c His atc Ile 245 gag G iu g ca Ala acc Thr tat Tyr g cc Ala 325 gtg ValI 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 ctg ggt ggg gca tgt ttg gtt Leu Gly Giy Ala Cys Leu Val atg ctc ggc Met Leu Gly WO 01/00805 WO 0100805PCTIBOOIOO926 345 350 355 att cgt atc tgg caa gac aac aag gtc aac ttc aac aat cca gtg aat 1219 Ile Arg Ile Trp Gin Asp Asn Lys Val Asn Phe Asn Asn Pro Vai Asn 360 365 370 ctq acc atg gct gcc gtt gct ttgq tt gca ggc att ggt aac ctc acc 1267 Leu Thr Met Ala Ala Val Ala Leu Val Ala Gly Ile Gly Asn Leu Thr 375 380 385 ctc acc gtt ttc gga gtc act ctt gaa ggc atc gca tgg qgc tct gta 1315 Leu Thr Val Phe Gly Val Thr Leu Giu Gly Ile Ala Trp Gly Ser Val 390 395 400 405 ggc atc att gtg ctq tac cca atc atq aag cgc ctg tac ctt tcc att 1363 Gly Ile Ile Val Leu Tyr Pro Ile Met Lys Arg Leu Tyr Leu Ser Ile 410 415 420 ggg gaa ggc aag aac gca aag ttc tagtacgctg ctaaagtatg cag 1410 Gly Giu Gly Lys Asn Ala Lys Phe 425 <210> 620 <211> 429 <212> PRT <213> Corynebacterium giutarnicum <400> 620 Val Leu Val Thr Ser Thr Trp Giy Trp Thr Val His Giy Asp Gly Lys 1 5 10 Lys Ilie Glu Pro Gly Ala Val Val Ala Pro Lys Glu Arg Leu Ser Trp 25 Gly Arg Thr Ilie Gly Ilie Gly Met Gin His Val Ile Ala Met Phe Gly 40 Ala Thr Leu Leu Val Pro Thr Leu Thr Gly Phe Pro Vai Asn Thr Thr 55 Leu Leu Phe Ser Giy Leu Gly Thr Ile Leu Phe Leu Leu Ile Thr Arg 70 75 Asn Arg Leu Pro Ser Tyr Leu Gly Ser Ser Phe Ala Phe Ile Ala Pro 90 Leu Thr Ala Thr Gin Val His Gly Ile Gly Val Gin Ile Gly Gly Ile 100 105 110 Leu Val Ala Gly Leu Val Leu Val Ala Ilie Gly Phe Val Val Lys Ala 115 120 125 Ala Gly Lys Arg Val Ilie Asp Ala Val Met Pro Pro Ala Val Thr Gly 130 135 140 Ala Ile Vai Ala Leu Ile Gly Leu Asn Leu Ala Pro Thr Ala Ala Gly 145 150 155 160 Asn Phe Ser Ser Gin Pro Leu Val Ala Thr Ala Thr Leu Phe Ala Ile 165 170 175 WO 01/00805 WO 0100805PCTIBOOIOO926 Leu Ile Ala Thr Val Ala Gly Arg Giy Met Ile Ala Arg Leu Gly Ile 180 185 190 Leu Ile Giy Val Val Ile Gly Trp Val Phe Ala Ala Ile Thr Giy Asn 195 200 205 Leu Ser Giu Giy Ala Ala Asp Thr Ile Arg Giu Ala Ala Trp Phe Gly 210 215 220 Leu Pro Gin Phe His Lys Pro Giu Phe Gin Leu Ser Ala Ile Leu Val 225 230 235 240 Thr Leu Pro Vai Ile Ilie Vai Leu Ile Aia Giu Asn Val Giy His Val 245 250 255 Lys Ala Val Ser Giu Met Thr Gly Glu Asp Leu Asp Asp Leu Ala Gly 260 265 270 Asp Ala Leu Ilie Ala Asp Gly Phe Gly Thr Thr Leu Ala Giy Ala Phe 275 280 285 Gly Gly Ser Gly Thr Thr Thr Tyr Ala Giu Asn Ile Gly Val Met Ala 290 295 300 Ala Thr Arg Val Tyr Ser Thr Ala Ala Tyr Trp Val Ala Ala Cys Thr 305 310 315 320 Ala Ilie Ala Leu Ala Phe Ile Pro Lys Phe Gly Ala Leu Ile Phe Thr 325 330 335 Ilie Pro Ala Gly Val Leu Gly Gly Ala Cys Leu Val Leu Tyr Gly Leu 340 345 350 Ilie Gly Met Leu Gly Ilie Arg Ile Trp Gin Asp Asn Lys Val Asn Phe 355 360 365 Asn Asn Pro Val Asn Leu Thr Met Ala Ala Val Ala Leu Val Ala Gly 370 375 380 Ilie Gly Asn Leu Thr Leu Thr Val Phe Gly Vai Thr Leu Giu Gly Ile 385 390 395 400 Ala Trp Gly Ser Val Gly Ile Ile Val Leu Tyr Pro Ile Met Lys Arg 405 410 415 Leu Tyr Leu Ser Ile Gly Giu Gly Lys Asn Ala Lys Phe 420 425 <210> 621 <211> 1335 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1312) <223> FRXA02233 <400> 621 WO 01/00805 atccccaccc aattggccca agagctgcag agitacgttg ctgtcgattt ccaagagcc cccttgggag tcgataatta PCTIIBOO/00926 tagaacccac ctctgcctaa aca Thr gca Ala atc Ile ccc Pro ctg Leo tac Tyr gic Val1 gig Val att Ile atc Ile 150 cca Pro g ca Ala atc Ile t gg T rp gt ValI ggt Gly a ca Th r g~g a Gly Ct 9 Le u cat His ci c Le u gati Asp 135 ggc Gi y ci g Leu ggc Gly ggc Gly qga Gly gic ValI atg Met cic Leo aca Th r gqt Gly ggc Gly giC Val 120 gct Ala ct 9 Leu gt Val cg c Arg igg Trp 200 t 99 Trp gc t Ala cag Gin a cc Thr atc Ile agt Ser att Ile 105 9CC Al a gtc Val aac As n gcc Al a gga Gly 185 gt ValI acc Th r cci Pro ca c His gga Gly cig Leo tct Se r ggc Gly ati Ile aig Met ctg Leu a cg Thr 170 aig Met ttc Phe gtc cac Val His aaa gag Lys Glu gig atc Val Ile iii ccg Phe Pro 60 tic cig Phe Leo 75 iii gct Phe Ala gig cag Val Gin gga ttt Gly Phe cca ccc Pro Pro 140 gca cca Ala Pro 155 gcg acc Ala Thr ati gct Ile Ala gca gci Ala Ala gga gac Gly Asp cgc cig Arg Leo 30 9CC aig Ala Met 45 gic aac Val Asn tig aic Le Ile tic at Phe Ile aic ggt Ile Gly 110 gig gig Val Val 125 gct gtc Ala Val acc gcg Thr Ala cic iii Leu Phe cgc ctg Arg Leu 190 aic acc Ile Thr 205 ggc Gly 15 a gc Se r tc Phe acc Thr acc Th r gca Al a 95 ggc Gly aaa Lys acc Th r gca Ala gcc Al a 175 ggc Gi y Gly aaa Lys t 99 Trp ggc Gly a ca Thr aga Arg 80 cci Pro ati Ile gca Ala ggi Gly gga Gly 160 at Ile ati Ile aac Asn gig Val1 1 aag Lys 999 Gly gcc Ala ct Leo aa c Asn ita Leo cii Leo 9gg Ala 9 cg Ala 145 aac As n tig Leo tig Leo cic Leo aic Ile cgc Arg a cg Thr ta Leo cga Arg acc Thr gi c Val1 Gly 130 aic Ile tc Phe aic Ile atc Ile ica Ser 210 cic gig act ica Leo Val Thr Ser gaa Glu aca Th r ci c Leo tc Phe cia Leo gca Ala 9 ca Ala 115 aaa Lys gig Val1 tcc Ser gci Ala gg i Gly 195 gaa Glu ccc Pro at Ile ctg Leo ici Se r ccc Pro acc T hr 100 99 t Gly cgc Arg 9 ca Ala agc Se r acc Thr 180 gig Val ggc Gly ggc Gi y gga Gi y gt Val1 gg t Gly i cg Ser caa Gin cic Leo gitt ValI cic Leo caa Gin 165 gt t ValI gig Val gca Ala 115 163 211 259 307 355 403 451 499 547 595 643 691 739 gca gac acc aic cgt Ala Asp Thr Ile Arg 215 gaa gca gca tgg tic gga Glu Ala Ala Trp Phe Gly 220 cig cca cag tic cac Leo Pro Gin Phe His 225 WO 01/00805 WO 0100805PCTJIBOO/00926 aag ccg gaa Lys Pro Giu 230 atc gtg ctc Ile Vai Leu atg aca ggg Met Thr Gly gac gga ttc Asp Giy Phe 280 acc acc tac Thr Thr Tyr 295 tcc acc gct Ser Thr Ala 310 ttc atc ccc Phe Ile Pro ctg ggt ggg Leu Gly Giy att cgt atc Ile Arg Ile 360 ctg acc atg Leu Thr Met 375 ctc acc gtt Leu Thr Val 390 ca g Gin gct Aia 250 ga c Asp acc Thr ga a Giu tac Tyr ttc Phe 330 tgt Cys caa Gin gcc Ala gga ctc Le u 235 gaa Giu ctc Le u acc Th r aa c As n tgg Trp 315 ggt Giy ttg Le u ga c Asp gt t Val1 gtc gcc att ttg gtg Aia Ile Leu Vai 240 gtc ggc cac gtc Vai Gly His Val 255 gac ctc qcc ggc Asp Leu Aia Gly 270 gca ggt gcc ttc Ala Giy Aia Phe 285 ggc gtc atq gcg Giy Val Met Aia gcc gcg tgc act Aia Ala Cys Thr 320 ctq atc ttc acc Leu Ile Phe Thr 335 ctt tac ggc cta Leu Tyr Giy Leu 350 aag gtc aac ttc Lys Vai Asn Phe 365 ttq gtt gca ggc Leu Vai Aia Gly ctt gaa ggc atc ctg Le u gca Aia gca Aia gga Giy 290 a cc Th r atc Ile ccc Pro 9gt Giy aat Asn 370 99 t Giy cca Pro gt c Vai ctt Le u 275 tcc Se r cg c Arg gcc Aila 9cc Ala atg Met 355 cca Pro aac Asn gtc Val1 t ca Se r 260 atc Ile Giy g ta Val1 ctt Le u gyC Gly 340 ctc Le u gtg Vali ctc Leu 835 883 931 979 1027 1075 1123 1171 1219 1267 1312 1335 tgg gct ctg Trp Ala Leu Phe Giy Vai Thr Leu Glu Giy Ile taggcatcat tgtgctgtac cca <210> 622 <211> 404 <212>' PRT <213> Corynebacterium giutamicum <400> 622 Val Leu Val Thr Ser Thr Trp Gly Trp Thr Val His Giy Asp Gly Lys 1 5 10 Lys Ile Giu Pro Giy Ala Val Val Ala Pro Lys Giu Arg Leu Ser Trp 25 Gly Arg Thr Ile Giy Ile Gly Met Gin His Vai Ile Ala Met Phe Gly WO 01/00805 PCT/EBOO/00926 40 Ala Thr Leu Leu Val Pro Thr Leu Thr Giy Phe Pro Vai Asn Thr Thr 55 Leu Leu Phe Ser Gly Leu Gly Thr Ile Leu Phe Leu Leu Ile Thr Arg 70 75 Asn Arg Leu Pro Ser Tyr Leu Gly Ser Ser Phe Ala Phe Ile Ala Pro 90 Leu Thr Ala Thr Gin Val His Gly Ile Gly Val Gln Ile Gly Gly Ile 100 105 110 Leu Val Ala Gly Leu Val Leu Val Ala Ile Gly Phe Vai Val Lys Ala 115 120 125 Ala Giy Lys Arg Val Ile Asp Ala Vai Met Pro Pro Ala Val Thr Gly 130 135 140 Ala Ilie Val Ala Leu Ile Gly Leu Asn Leu Ala Pro Thr Ala Ala Gly 145 150 *155 160 Asn Phe Ser Ser Gin Pro Leu Val Ala Thr Ala Thr Leu Phe Ala Ile 165 170 175 Leu Ile Ala Thr Val Ala Gly Arg Gly Met Ile Ala Arg Leu Gly Ile 180 185 190 Leu Ilie Gly Val Val Ile Gly Trp Val Phe Ala Ala Ile Thr Gly Asn 195 200 205 Leu Ser Glu Gly Ala Aia Asp Thr Ile Arg Giu Ala Ala Trp Phe Gly 210 215 220 Leu Pro Gin Phe His Lys Pro Giu Phe Gin Leu Ser Ala Ile Leu Val 225 230 235 240 Thr Leu Pro Val Ile Ile Vai Leu Ile Ala Giu Asn Val Gly His Val 245 250 255 Lys Ala Val Ser Glu Met Thr Gly Giu Asp Leu Asp Asp Leu Ala Gly 260 265 270 Asp Ala Leu Ile Ala Asp Gly Phe Gly Thr Thr Leu Ala Gly Ala Phe 275 280 285 Gly Gly Ser Gly Thr Thr Thr Tyr Ala Giu Asn Ile Gly Val Met Ala 290 295 300 Ala Thr Arg Val Tyr Ser Thr Ala Ala Tyr Trp Val Ala Ala Cys Thr 305 310 315 320 Ala Ile Ala Leu Ala Phe Ile Pro Lys Phe Gly Ala Leu Ile Phe Thr 325 330 335 Ile Pro Ala Gly Val Leu Gly Gly Ala Cys Leu Val Leu Tyr Gly Leu 340 345 350 Ile Gly Met Leu Gly Ile Arg Ile Trp Gin Asp Asn Lys Val Asn Phe 355 360 365 WO 01/00805 PCTIIBOO/00926 Asn Asn Pro Val Asn Leu Thr Met Ala Ala Val Ala Leu Val Ala Gly 370 375 380 Ile Gly Asn Leu Thr Leu Thr Val Phe Gly Val Thr Leu Glu Gly Ile 385 390 395 400 Ala Trp Ala Leu <210> 623 <211> 2010 <212> DNA '213> Corynebacteriun glutamicun <220> <221> CDS <222> (1987) <223> RXN02372 <400> 623 gacaatactg atggataaat ttcatatcga ggacgaaggg acaaccccga acgccgtgac aacatcaacc acaacccggg tgaaacatcc ggtagaccag gtg cca ccc gca ccc Val Pro Pro Ala Pro aaa Lys gcc Ala gcg Ala gcc Ala c ta Le u gcc Ala cct Pro g ca Ala ac gca Ala att Ile acc Th r ttg Le u atc Ile gg t Gly at Ile C cg Pro 120 act qc C Al a gtt Val att Ile att Ile gt t Val ttg Le u tac Tyr 105 gtg Val1 gtg cta Leu cCg Pro cac His cag Gin caa Gin gg C Gly gg t Gly ttt Phe ct g ggg Gly ctg Le u Ctg Le u tct Ser ggt Gly 75 gtt ValI g ca Ala gcq Ala ttg ctc Le u ctg Le u att Ile gt g ValI gtc Val act Thr gtc ValI cgt Arg gtt caa Gin att Ile aac As n 45 9gc Gly acc Thr gat Asp att Ile ttc Phe 125 atg cac His g ca Ala 30 gc t Ala att Ile act Thr ggt Gly gtc ValI 110 ct C Le u ggt gt t ValI 15 cag Gin qa c Asp gg t Gly act Th r caa Gin 95 tcc Ser aag Lys gct ct t Le u t cg Se r ttg Le u cq C Arg g ct Ala 80 gg t Gly gg C Gly ttc Phe t CC 1 qct Ala ct g Leu ttg Le u cac His gtt Val1 Gly att Ile ttc Phe ctg ttc Phe aac As n aca Thr att Ile gCt Ala gtt Val ttc Phe cca Pro 130 ct g tac gca Tyr Ala ttg gac Leu Asp tgt ggc Cys Gly ggt qtg Gly Val ccc atc Pro Ile gcg tcg Ala Ser 100 acg ttC Thr Phe 115 cca gtt Pro Val tcg gta gg a Gi y act Thr atc Ile cgC Arg atc Ile ctg Le u ttt Phe gtc Val1 tCt 115 163 211 259 307 355 403 451 499 547 WO 01/00805 PCT/LB00100926 Thr Gly Thr Val Leu Leu Val Met Gly Ala Ser Leu Leu Ser Val Ser gca Al a 150 cii Le u tic Phe ggt Gly gig Val gtt Val1 230 atc Ilie atc Ile gct Ala tac Tyr aaa Lys 310 ggt Gly gtc Val1 gg C Gly gic Val1 ga c Asp tac Tyr cg i Arg acc Thr 200 aai As n ga a Glu acc Th r ggc GI y gg C Gly 280 tgc Cys cg C Arg ctg Le u ggt Gly cag Gln 360 gig Val1 ttt Ph e ggi Gl y gga Gly 185 gca Al a gct Ala ttt Phe at g Met aag Lys 265 ct g Le u ttC Phe tgg Trp ccc Pro gg C Gly 345 acc Th r acc Th r 140 145 gtg ValI ttt Phe 170 tic Phe gt t Val1 gaa Giu aac As n gtg Val 250 cg a Arg icc Se r gcg Ala gt Val1 aag Lys 330 g ca Ala a ic Ile icc Se r gcc Ala tg Leu acc Tb r atc Ile 205 ga C Asp gcc Ala acc Th r cg c Arg atg Met 285 gt t Val gct Ala gcg Ala gca Ala tct Se r 365 ggc Gly ga t Asp gcg Ala tig Leu 190 ttg Le u atc Ile ati Ile gg t Giy agt Se r 270 gg t Gly ggc Gly gct Ala atc Ilie cig Le u 350 ga C Asp cia Le u gggi Gly gig Val1 175 gct Ala gg c Gly acc Th r tc Phe ga t Asp 255 ga t Asp ggc Gi y ci g Le u gcc Ala gtc ValI 335 ttC Ph e ctc Leu gcc Ala gig Val1 160 atc Ile gig Val1 gati Asp act Thr icc Se r 240 gig Val1 gi c Val1 gic ValI gig Val1 Gly 320 gct Ala gcc Ala gct Ala atg Met cci Pro ati Ile t ig Le u gcc Ala ccg Pro 225 aig Met tt Phe acc Thr aig Met cg C Arg 305 tic Phe icc Ser aac As n gat Asp ci g Le u gct Ala t ig Le u atc Ile aac As n 210 iii Phe at Ile gca Ala cgc Arg aac As n 290 ate Ile atg Meti aic Ile gt Val1 agc Se r 370 gig Val1 cgc Arg cag Gin 180 cig Leu gat Asp iii Phe gic Val1 ggg Gly 260 cig Le u tic Phe ggC Gly ate Ile icc Se r 340 i gg Trp aac As n tic Phe gati Asp 165 cg c Arg gt Val1 gag Giu gg t Gly aig Met 245 ga a Giu cg c Arg ceg Pro gig Val1 etc Le u 325 cca Pro gig Val1 icc Se r cgc Arg 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 WO 01/00805 PTIO/02 PCTABOO/00926 ccc Pro 390 tcc Se r ttC Phe tcc Ser ga c Asp ttg Le u 470 cgc Arg gaa Glu acc Th r ctt Le u gag Glu 550 acc Th r ga c Asp gag Glu gcc Ala ca g Gin gtc Val 410 gga Gly atc Ile acc Th r t gg Trp ca a Gin 490 cac His gcc Ala ga t Asp ga a Glu gtg Val1 570 gag Glu ga c Asp ttc Phe gcg Ala 395 9gc Gly cgc Arg aat Asn ttc Phe ga a G lu 475 gtc Val1 ga c Asp g cc Ala ga c Asp Cgg Arg 555 ga t Asp cag Gin cgc Ar g gat Asp ttc Phe gcg Al a caa Gin ttg Le u gca Al a 460 tcc Se r gct Ala tac T yr aco Thr gtg Val 540 ttc Phe acc Thr gag Glu ttc Phe cg c Arg 620 gcg Ala 400 atc Ile caa Gin aa c Asn aa c As n gcc Ala 480 gca Ala g ca Al a ga c Asp aag Lys tat Tyr 560 gcc Ala gcg Ala ct t Le u gag Glu cg t Arg ctg Le u gt c Val aag Lys agc Ser 4 aac Asn C cg Pro cag Gln cgt Arg ctc Leu 545 gt t Val1 ggC Gly cta Leu gcc Ala act Thr 625 t cc S er 405 ctg Le u aaa Lys acc Thr cc t Pro ctg Le u 485 cgc Arg gcc Ala ggt Gly acc Th r ga c Asp 565 ctc Le u att Ile cca Pro ggc Gly 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 WO 01/00805 taaaacacca aaaacaaatt aag <210> 624 <211> 629 <212> PRT <213> Corynebacteriun giutanicurn <400> 624 Val Pro Pro Ala Pro Lys Leu Ala Ala PCTIBOO/00926 2010 1 Ala Le u Leu His Val1 Gly Ile Phe Le u 145 Pro Ile Leu Ala Pro 225 Met Tyr Le u Cy s Gi y Pro Ala Thr 115 Pro Ser Ala Ala Gly 195 Le u Tyr Ile Ala Asp Gly Val1 Ile Ser 100 Phe Val1 ValI Ar g Gin 180 Leu Asp Phe Val Gly 260 5 Gly Thr Ile Arg Ile Le u Phe Val1 Ser Asp 165 Arg Val Giu Gly Met 245 Al a Ala Ala Le u 70 Ala Pro Ala Th r Ala 150 Le u Phe Gly Val1 Val 230 Ile Val1 Thr Thr Pro Ile Al a Ala Gly 135 As n Ala Phe Gly Gly 215 Pro Ile Ile Tb r 40 Le u Ile Gly Ile Pro 120 Tb r Asp Tyr Arg Tb r 200 Asn Giu Thr Val1 25 Ile Ile Val1 Le u Tyr 105 Val1 Val Phe Gly Gly 185 Ala Ala Phe Met L ys 265 Leu 10 Pro His Gin Gin Gly 90 Gly Phe Le u ValI Phe 170 Phe Val1 Giu As n Val1 250 Giy Le u Le u Se r Gly 75 Val1 Ala Ala Le u Asn 155 Gly Met Al a Ala Al a 235 Glu Le u Le u Ile Val Val Thr Val Arg Val1 140 T yr Th r Gly Leu Phe 220 Val Thr Gin Ile As n Gly Thr Asp Ile Phe 125 Met Ala Le u Thr Ile 205 Asp Al a Thr His Ala Ala Ile Thr Gly Val1 110 Le u Gly Asp Al a Le u 190 Leu Ile Ile Gly Val Gin Asp Gly Th r Gin Se r L ys Ala Gly ValI 175 Ala Gly Thr Ph e Asp 255 Le u Ser Le u Ar g Ala Gly Gly Ph e Ser Val 160 Ile Val1 Asp Thr Se r 240 Val1 Phe Ala Thr Giu Ile Val Gly Arg Thr Arg Arg Ser Asp Val 270 WO 01/00805 PCT[IBOO/00926 Thr Arg Ala Leu Arg Ala Asp Gly Leu Ser Thr Leu Met Gly Gly Val Met Arg 305 Phe Ser As n Asp Leu 385 Arg Le u ValI Lys Se r 465 As n Pro Gin Arg Le u 545 Vai Giy Le u As n 290 Ile Met Ile Val1 Se r 370 Val1 Ilie Leu Ala Met 450 Lys ValI Le u Le u Gly 530 Ile Ala Le u Ser 275 Ser Thr Ilie Pro Ala 355 Arg Se r Phe As n Th r 435 Asp Thr Thr Se r Ile 515 Ser Thr Tyr Arg Giu 280 285 Phe G ly Ilie Se r 340 T rp Asn Phe Val1 Leu 420 Se r Arg Trp Giu Asp 500 Leu Ile Val1 Phe Arg 580 Ile Pro Val Le u 325 Pro Vai Se r Arg Se r 405 Le u Lys Tb r Pro Le u 485 Arg Aia Gly Th r Asp 565 Le u Ile Tyr Lys 310 Gly Val1 Gly Vali Pro 390 Se r Phe Ser Asp Leu 470 Arg Glu Thr Le u Giu 550 Thr Asp Glu Thr 295 Se r Val Le u Ile Ile 375 Asp Gly Phe Gly Ph e 455 Giu Glu Glu Giu Asp 535 Gin Met Asn Ile Cys Arg Le u Gly Gin 360 Vali Vali Met His Giu 440 Vali Thr Ala Le u Giu 520 Asp Tyr Asp Se r Ala Phe T rp Pro Giy 345 Thr Thr Aia Se r Vali 425 Arg Giu Aia Ilie Ile 505 Gi u Le u Arg Se r Asp 585 Asn Ala Vai Lys 330 Ala Ile Ser Gin Vali 410 Giy Ile Tb r T rp Gin 490 His Aia Asp Giu Val 570 Giu Asp As n 300 Ala Gly Le u Lys Le u 380 Phe Ala Gin Le u Ala 460 Se r Ala Tyr Tb r Vai 540 Phe Thr Glu Phe Val Giy Aia Ala Ala Ile Ala Leu 350 Ser Asp 365 Gly Leu Pro Giu Ilie Thr Ser Giy 430 Asp Ala 445 Pro Leu Gin Pro Vai Leu Pro Asp 510 Ile Ser 525 Asp Gin Asn Met Val Vai His Arg 590 Asp Ile Le u Ala Vai 335 Phe Le u Ala Trp Ala 415 Giy Vali Phe Phe Th r 495 Met Gin Giu Pro Ala 575 Gin Leu Vai Gi y 320 Ala Ala Ala Met Ala 400 Ile Gin As n Asn Ala 480 Ala Ala Asp Lys Tyr 560 Ala Ala Le u WO 01/00805 595 600 605 Asp Ala Asn Pro Ala Arg Ser Ala Phe Asp Arg Lys Phe Thr Glu 610 615 620 Asp Phe Leu Gly TIIBOO/00926 Ala Thr 625 <210> 625 <211> 578 (212> DNA <213> Corynebacterium giutarnicum <220> <221> COS <222> <223> FRXA02372 <400> 625 gat gcg gtc aac aag Asp Ala Val Asn Lys 1 5 ccg ctg ttc aac age Pro Leu Phe Asn Ser cag cca ttc gcc aac Gin Pro Phe Ala Asn gtcg etc acc gca ccg Val Leu Thr Ala Pro ccc gac atg gca cag Pro Asp Met Ala Gin atc tct caa gac egt Ile Ser Gin Asp Arg gat caa gaa aag ctc Asp Gin Giu Lys Leu 100 aac atg ccg tat gtt Asn Met Pro Tyr Val 115 gte gta gee gcc ggc Vai Vai Ala Ala Giy 130 cac cgc caa gcg cta His Arg Gin Ala Leu 145 atg Met aag Lys qt e Val1 ttg Le u ct c Le u 70 ggt Gly atc Ilie g cg Ala ttg Le u t cg Se r 150 gat cgc Asp Arg acc tgg Thr Trp acg gaa Thr Giu 40 tcc gac Ser Asp 55 att ttg Ile Leu tcg atc Ser Ile acc gte Thr Vai tac ttc Tyr Phe 120 ege ege Arg Arg 135 gaa atc Giu Ile gac Asp 10 ttg Leu eq e Arg gaa Giu ace Thr ctt Le u 90 gag Glu ace Thr gac Asp gag Glu ttc Phe ga a Giu gaa Giu gag Giu gaa Giu 75 ga t Asp ca g Gin atq Met aac Asn att Ile 155 cgt gta Val1 ace Th r gee Ala etc Le u gag Giu gat Asp tac Tyr gat Asp tee Ser 140 gee Ala tea gaa Glu gca Ala ate Ile ate Ile gaa Giu cte Leu ege Arg tet Se r 125 ga e Asp aat Asn get aee Thr tgg Trp caa Gin cac His gee Ala ga t Asp gaa Glu 110 gtg Val1 gag Giu gac Asp tte tte Phe ga a Giu gte Val ga c Asp gee Ala gac Asp egg Arg ga t Asp eag Gin eg c Arg ga t gea Ala tee Se r get Ala tac T yr ace Thr gt g Val1 tte Phe ace Thr gag Giu t te Phe 160 ege 48 96 144 192 240 288 336 384 432 480 528 gat ata ttg ett gee gac get aae eca gee WO 01/00805 PCT11B00100926 Asp Ilie Leu Leu Ala Asp Ala Asn Pro Ala Arg Ser Ala Phe Asp Arg 165 170 175 aag ttt acc gag act gac ttc ctc ggc taaaacacca aaaacaaatt 575 Lys Phe Thr Giu Thr Asp Phe Leu Giy 180 185 aag 578 <210> 626 <211> 185 <212> PRT <213> Corynebacteriui giutarnicum <400> 626 Asp Pro Gin Val1 Pro Ile Asp As n Val His 145 Asp Ala Val Asn Lys Met Asp Arg Thr. Asp Phe Val Giu Thr ?he Ala Asn Ser Ala Asn Ala Pro Ala Gin Asp Arg Lys Leu 100 Tyr Val Ala Gly Ala Leu Leu Ala 165 Lys Val1 Le u Le u 70 Gly Ile Ala Le u Ser 150 Asp Thr T hr Ser Ile Ser Thr Tyr Arg 135 Glu Ala T rp Giu Asp Le u Ile Val Phe 120 Arg Ile Asn Pro 25 Le u Arg Al a Gly T hr 105 Asp Le u Ile Pro 10 Le u Arq Giu Thr Le u 90 Giu Th r Asp Gi u Ala 170 Giu Giu Giu Glu 75 Asp Gin Met Asri Ile 155 Arg Th r Ala Le u Glu Asp T yr Asp Se r 140 Ala Ser Ala Ile Ile Giu Leu Arg Se r 125 Asp Asn Ala Trp Gin His Al a Asp Glu 110 Val Giu Asp Phe Glu Vai Asp Ala Asp Arg Asp Gin Arg Asp 175 Se r Ala T yr Thr Val1 Phe Th r Giu Phe 160 Arq Lys Phe Thr Giu Thr Asp Phe Leu 180 <210> 627 <211> 1314 <212> DNA <213> Corynebacterium glutarnicum <220> <221> CDS <222> (1291) WO 01/00805 <223> FRXA02377 <400> 627 gacaatactg aiggaiaaai iicatatcga ggacgaaggg PCTIBOOIOO926 acaaccccga acgccgtgac aacaicaacc acaacccggg tgaaacatcc ggtagaccag gig cca. ccc gca ccc Val Pro Pro Ala Pro aaa Lys gcc Al a gcg Ala 9cc Ala cta Le u gcc Ala cci Pro gca Ala acc Thr g ca Ala 150 ctt Le u ttc Phe cti Le u gic Val1 acc Thr acg Th r ccg Pro at Ile gcc Ala gcg Ala gg i GI y 135 aai As n gci Ala tic Phe gca Ala at Ile acc Th r i iq Le u aic Ileggi Gly at I le ccg Pro 120 aci Thr ga c Asp iac Tyr cgi Arg gcc Ala gt Val1 aii Ile ati Ile Val1 tig Leu iac T yr 105 gig Val1 gig Val1 iii Phe ggi Gly gga Gly 185 cia Leu ccg Pro cac His cag Gin caa Gin Gly gi Gly iii Phe cig Le u gig Val iii Phe 170 tic Phe ggg9 Gly cig Le u ci g Le u ici Se r gg i Gly 75 gii Val1 g ca Ala gcg Ala tig Le u aac Asn 155 gg c Gly aig Meti cic Leu cig Leu aii Ile gig Val1 60 gi c Val aci Thr gi c Val1 cgi Arg gii Val 140 tac Tyr acc Thr ggc Gly caa Gln aii Ile aac As n 45 gg c Gly acc Thr gati Asp aii Ile iic Phe 125 aig Meti gcc Ala iig Le u acc Thr ca c His gca Ala .30 gct Ala at Ile aci Th r gg i Gly gtc ValI 110 cic Le u 99 i Gly gai Asp g cg Ala tig Leu 190 gi i Val1 15 cag Gln gac Asp ggi Gly aci Thr caa Gin 95 icc Ser aa g Lys gct Ala ggg Gly gig Val1 175 gct Ala ggc Gly cii Le u i cg Se r i ig Leu cg c Arg gci Ala 80 ggi Gly ggc Gly iic Phe icc Se r gtg Val1 1 6C aic Ile gtcj Va l 1 gci Ala ctg Le u iig Leu cac His gi i Val ggc Gi y aii Ile iic Phe ci 9 Le u 145 cci Pro aii Ile tig Leu iic Phe aac As n aca Thr ati Ile gci Ala gi i Val1 iic Phe cca Pro 130 ct g Le u gci Ala iig Le u aic Ile iac Tyr i ig Leu i gi Cys ggi Gly ccc Pro gcg Ala acg Thr 115 cc a Pro i cg Ser g cc Al a g cg Ala ggc Gi y 195 g ca Ala ga c As p ggc Gly gig Val aic Ile i cg Ser 100 iic Phe gii ValI gia ValI cg c Arg cag Gin 180 cig Le L gga Gl y aci Thr aic Ile cgc Ar g atc Ile ci g Le u iii Phe gic Val1 ici Se r gati Asp 165 cgc Arg gii Val 115 163 211 259 307 355 403 451 499 547 595 643 691 739 ggi ggc acc gca Gly Gly Thr Ala 200 gii gci cig aic tig Val Ala Leu Ile Leu 205 gat gcc aac iig gat gag Asp Ala Asn Leu Asp Glu 210 gig Val1 gitt Val1 230 atc Ile atc Ile gct Ala tac Tyr aaa Ly s 310 ggi Gly gi c Val1 99c Gly gic Val1 WO 01/00805 gga aat gci Gly Asn Ala 215 cca gaa ttt Pro Giu Phe aic acc atg Ile Thr Met gtc ggc aag Val Gly Lys 265 gac ggc cig Asp Gly Leu 280 acg tgc tic Thr Cys Phe 295 tci cgc igg Ser Arg Trp gig cig ccc Val Leu Pro cic ggi ggc Leu Gly Gly 345 aic cag acc Ile Gin Thr 360 aic gig acc Ilie Val Thr 375 gaa Glu aa c As n gig Val1 250 cqa Arg icc Se r gc g Al a gi i Val1 aag Lys 330 g ca Ala aic Ile icc Se r gcg Al a gct Al a 235 gag Glu act Th r a cc Thr caa Gl n gcg Al a 315 gct Ala icc Se r gcc Ala gca Ala tic Phe 220 gii Val1 acc Th r cg c Arg ctg Le u aac As n 300 gca Ala ggc Gly tig Le u aag Lys cii Le u 380 ga c Asp gcc Ala acc Thr cgc Arg aig Meti 285 gii Val1 g ci Ala g cg Ala g ca Ala ict Se r 365 gg c Gly iii Phe at Ile gca Ala cgc Arg aac Asn 290 aic Ile aig Meti aic Ile gt Val1 a gc Se r 370 gig Val tat Tyr aic Ile a cg Thr gca Ala 275 icc Se r acg Th r aic Ile cci Pro gca Ala 355 cgc Arg icC iii Phe gic Val1 ggg Gly 260 cig Le u tic Phe ggc Gly aic Ile icc Ser 340 i gg Trp aa c As n tic ggi Gly a ig Met 245 ga a Glu cgc Arg c cg Pro gig Val cic Le u 325 cca Pro gig Val1 icc Ser cgc T/EBO0100926 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1314 Leu Ala Met Leu Ser Phe Arg ccc gat git gci caa ggc git ccc igagigggcg cgtaiciicg ici Pro Asp Val Ala Gln Val Pro <210> 628 <211> 397 <212> PRT <213> Corynebacierium giutamicum <400> 628 Val Pro Pro Ala Pro Lys Leu Ala Ala Leu Gly Leu Gln His Val Leu 1 5 10 Ala Phe Tyr Ala Gly Ala Val Ile Val Pro Leu Leu Ile Ala Gin Ser 25 WO 01/00805 PCT/IB00/00926 Leu Asn Leu Asp Thr Ala Thr Thr Ile His Leu Ile Asn Ala Asp Leu 40 Leu Thr Cys Gly Ile Ala Thr Leu Ile Gin Ser Val Gly Ile Gly Arg 55 His Ile Gly Val Arg Leu Pro Ile Val Gin Gly Val Thr Thr Thr Ala 70 75 Val Ala Pro Ile Ile Ala Ile Gly Leu Gly Val Thr Asp Gly Gin Gly 90 Gly Val Ala Ser Leu Pro Ala Ile Tyr Gly Ala Val Ile Val Ser Gly 100 105 110 Ile Phe Thr Phe Phe Ala Ala Pro Val Phe Ala Arg Phe Leu Lys Phe 115 120 125 Phe Pro Pro Val Val Thr Gly Thr Val Leu Leu Val Met Gly Ala Ser 130 135 140 Leu Leu Ser Val Ser Ala Asn Asp Phe Val Asn Tyr Ala Asp Gly Val 145 150 155 160 Pro Ala Ala Arg Asp Leu Ala Tyr Gly Phe Gly Thr Leu Ala Val Ile 165 170 175 Ile Leu Ala Gin Arg Phe Phe Arg Gly Phe Met Gly Thr Leu Ala Val 180 185 190 Leu Ile Gly Leu Val Gly Gly Thr Ala Val Ala Leu Ile Leu Gly Asp 195 200 205 Ala Asn Leu Asp Glu Val Gly Asn Ala Glu Ala Phe Asp Ile Thr Thr 210 215 220 Pro Phe Tyr Phe Gly Val Pro Glu Phe Asn Ala Val Ala Ile Phe Ser 225 230 235 240 Met Ile Ile Val Met Ile Ile Thr Met Val Glu Thr Thr Gly Asp Val 245 250 255 Phe Ala Thr Gly Glu Ile Val Gly Lys Arg Thr Arg Arg Ser Asp Val 260 265 270 Thr Arg Ala Leu Arg Ala Asp Gly Leu Ser Thr Leu Met Gly Gly Val 275 280 285 Met Asn Ser Phe Pro Tyr Thr Cys Phe Ala Gin Asn Val Gly Leu Val 290 295 300 Arg Ile Thr Gly Val Lys Ser Arg Trp Val Ala Ala Ala Ala Ala Gly 305 310 315 320 Phe Met Ile Ile Leu Gly Val Leu Pro Lys Ala Gly Ala Ile Val Ala 325 330 335 Ser Ile Pro Ser Pro Val Leu Gly Gly Ala Ser Leu Ala Leu Phe Ala 340 345 350 Asn Val Ala Trp Val Gly Ile Gin Thr Ile Ala Lys Ser Asp Leu Ala WO 01/00805 PCTJ/iBOO/00926 355 360 365 Asp Ser Arg Asn Ser Val Ile Val Thr Ser Ala Leo Gly Leu Ala Met 370 375 380 Leu Val Ser Phe Arg Pro Asp Val Ala Gin Gly Val Pro 385 390 395 <210> 629 <211> 1512 (212) DNA <213> Corynebacieriun glutamicum <220> <221> CDS <222> (1489) <223> RXA02676 <400> 629 aaccattggg tattaagaaa tttgtggcti agatctcaat ttctgtatag tigatcata ctaattcatc caciiccaaa tticacgaa ggatcacccc atg gac acc tgg gaa Met Asp Thr Trp Glu caa Gin gcc Ala acc Th r gtc Val1 gcc Ala gtt Val1 atc Ile atc I le cca Pro Ct t Leu atc Ile ata Ile 9 ca Ala gtc Val1 aca Th r g9t Gly gga Gly 120 atc* Ile gga Gly t9 Leo Ctg Leo gta Val 9CC Al a tcc Ser ga a Glu 105 ttc Phe ttc Ph e aca Th r gti Val gtt Val gtg Val1 cta Leo gg t Gly aaa Ly s cci Pro gca Ala 999 Gly ctc Leo tca Ser gac Asp i tg Leo 75 99c Gly cga Arg atc Ile gta Val1 cca Pro gtc Val1 att Ile act Thr gta Val1 gca Ala gca Al a tc Phe gct Ala 140 ci 9 Leo aic Ile gt ValI 45 cic Leo 99 C Gly aaa Lys gct Ala itc Phe 125 cga Arg cta Leo gti Val 30 acc Thr cti Leo Cig Leo ict Se r ttc Phe 110 gati Asp Cc Arg ggc Gly 15 ttt Phe gca Al a ga c Asp g99 Gly cia Leo 95 gca Ala gC t Ala ci c Leo 1 ait gca Ile Ala aaa cic Lys Leo ctt gCt Leo Ala ggt tti Gly Phe 9CC aig Ala Met 80 gCC qac Ala Asp ctc ggt Leo Gly 99C ctc Gly Leo aac ggc Asn Gly 145 9CC Al a cat His 9CC Ala ggt Gl y ct Leo act Tb r gtC Val1 9tg Val 130 icc ggC Gly gC t Ala 99C Gly aaa Lys ggi Gly a tg Met 9 Ca Ala 115 gic Val1 gi c 9CC Ala ttt Phe ati Ile aca Thr cga Arg gtg Val1 100 tCg Ser a tg Met ctt att Ile Cc Leo

CCC

Pro ctc Leo ttg Leo cga Arg ctg Leo cic Leo act 115 163 211 259 307 355 403 451 499 547 Ser Val Leo Thr WO 01/00805 WO 0100805PCT/IB00/00926 iii ggt Phe Gly 150 cca cct Pro Pro git gga Val Gly tat tta Tyr Leu ctt ccc Leu Pro 215 cct cag Pro Gin 230 cct atg Pro Met gcc ggc Ala Gly ctc ggc Leu Gly tic tic Phe Phe 295 acc atc Thr Ile 310 ggc gcc Gly Ala gga gcg Gly Ala ggt tgt Gly Cys atc Ile cac His tac Tyr acc Thr 200 gta Val aac Asn cic Le u ci c Le u gaa Giu 280 cta Leu gaa Gi u gg t Giy ctt Le u tic Phe 360 cct Pro Cca Pro gia Val 185 ggt Gly ccc Pro cca Pro ctt Le u cta Le u 265 aca Th r ggc Gly ggc G ly ggc Giy qca Ala 345 atc Ile g ca Ala ggc Gly 170 cta Le u tac Tyr gat Asp gc t Ala att Ile 250 gat Asp c ca Pro aac As n gca Ala atg Met 330 ga c Asp gtg Val gct Ala 155 c Ca Pro atc Ilie cig Le u cta Leu aac Asn 235 ttt Phe gca Ala gtg Val cga Arg ctc Le u 315 ttc Phe tcc Ser gca Ala ggc Gly at Ile gct Ala cta Le u ctc Le u 220 gca Ala g gc Gly gaa Glu g ca Ala cgt Arg 300 qgc Gly gg t Giy atg Met gcc Al a gcc Ala gca Al a ggc Gly ggt Gly 205 agt Ser gig ValI aat Asn tcc Ser cig Le u 285 ggc Gi y cca Pro gga Gly gca Ala gtc Val 365 ttc tct Phe Ser gcc tct Ala Ser 175 atc atc Ilie Ile 190 aag tic Lys Phe ggt gga Gly Gly tcg atc Ser Ile acc gga Thr Gly 255 acc atg Thr Met 270 cic att Leu Ile att aat Ile Asn atc tgt Ile Cys gig cta Val Leu 335 gat cta Asp Leu 350 ctt cgt Leu Arg gi C Val1 160 ga a Glu gti Val ita Le u gca Ala att Ile 240 aca Thr gtg Val acc Th r gg i Gly ica Se r 320 cgc Ar g gga Gly gtc Val aig Met tc Phe gca Ala ggc Gly ca g Gin 225 gi c Val t ca Ser aaa Lys tig Leu tct Ser 305 gic Val a cg Th r ctt Le u g cg Ala cac His ttc Phe cta Le u cga Arg 210 ga a Giu att Ile a tg Met ati Ile aic Ile 290 gct Ala gta Val1 tct Ser cca Pro ca g Gin 370 gig Val1 Gly ccc Pro 195 aag Lys ga t Asp ttg Le u gca Ala cta Le u 275 att Ile cta Leu ta Le u gga Gly gtt Val1 355 gg t Gly tic Phe gca Al a 180 acc Thr itc Phe ga t Asp ctc Leu gt Val 260 gga Gly gcc Ala gag Giu atc Ile ati Ile 340 atc Ile t ci Ser gtc ValI 165 ca a Gin i gg Trp ccc Pro ca g Gin ati Ile 245 t cc Se r tc Phe cig Le u aaa Lys act Thr 325 gga Gly gca Ala gcc Ala 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 acc git gcg Thr Val Ala 375 cia- acc acc gcc gca gca cic atg Leu Thr Thr Ala Ala Ala Leu Met 380 cci gci gii gcc Pro Ala Val Ala WO 01/00805 gct gct gac ttt aac gaa ttc cag ctt Ala Ala Asp Phe Asn Giu Phe Gin Leu 390 395 9cc gct 99t tct gtt att gcc agc cac Ala Ala Gly Ser Val Ile Ala Ser His 410 ctc gtt ggt cga ctc atg aac gcc gac Leu Val Gly Arg Leu Met Asn Ala Asp 425 430 tgg acc qta aac caa acc tgc att gcg Trp Thr Val Asn Gin Thr Cys Ile Ala 440 445 tat gca atg ttc gga ttg gca tcg ct~t Tyr Ala Met Phe Giy Leu Ala Ser Leu 455 460 ttc <210> 630 <211> 463 <212> PRT <213> Corynebacterlum glutamicum <400> 630 Met Asp Thr Trp Glu Gin Thr Leu Gly PCTIIBOO/00926 gcc atc gtt att tcc act 1315 Ala Ile Val Ile Ser Thr 400 405 aac gac tcc gga ttc tgg 1363 Asn Asp Ser Gly Phe Trp 420 ccc acc acg cta aaa act 1411 Pro Thr Thr Leu Lys Thr 435 gtg gga ttt gtg atg gcc 1459 Val Giy Phe Val Met Ala 450 tagtccgctg accccattga 1509 1512 1 Ala Leu Ala Phe Met Asp Gly Le u Gly 145 Met Al a Phe Ile Thr Arg Val1 100 Se r Met Leu Phe 5 Ile Le u Pro Le u Le u Arg Le u Le u Th r Val1 165 Aia Thr Val1 Ala 70 Val1 Ile Ile Pro Phe 150 Pro Le u Le u Thr Se r Glu Phe Met Val 135 Gly Pro Ile Ile Ala Val Th r Gly Gly 120 Ile Ile His Leu 25 Le u Val Ala Ser Giu 105 Phe Phe Pro Pro Th r 10 Val1 Val1 Val1 Le u Gly 90 Lys Pro Ala Ala Gly 170 Gly Le u Se r Asp Leu 75 Gly Arg Ile ValI Al a 155 Pro Pro Val1 Ile Thr Val1 Ala Ala Phe Ala 140 Gi y Ile Leu Ile Val Le u Gly Lys Ala Phe 125 Arg Ala Ala Le u Val T hr Leu Leu Ser Phe 110 Asp Arg Phe Ala Gly Phe Ala Asp Gly Le u Ala Ala Le u Se r Se r 175 Ile Lys Le u Gly Ala Ala Leu Gly Asn Val1 160 Glu WO 01/00805 PCT/IB00/00926 Phe Phe Gly Ala Gin Val Gly Tyr Val Leu Ile Ala Gly Ile Ile Val 180 185 190 Ala Leu Pro Thr Trp Tyr Leu Thr Gly Tyr Leu Leu Gly Lys Phe Leu 195 200 205 Gly Arg Lys Phe Pro Leu Pro Val Pro Asp Leu Leu Ser Gly Gly Ala 210 215 220 Gin Glu Asp Asp Gin Pro Gin Asn Pro Ala Asn Ala Val Ser Ile Ile 225 230 235 240 Val Ile Leu Leu Ile Pro Met Leu Leu Ile Phe Gly Asn Thr Gly Thr 245 250 255 Ser Met Ala Val Ser Ala Gly Leu Leu Asp Ala Glu Ser Thr Met Val 260 265 270 Lys Ile Leu Gly Phe Leu Gly Glu Thr Pro Val Ala Leu Leu Ile Thr 275 280 285 Leu Ile Ile Ala Leu Phe Phe Leu Gly Asn Arg Arg Gly Ile Asn Gly 290 295 300 Ser Ala Leu Glu Lys Thr Ile Glu Gly Ala Leu Gly Pro Ile Cys Ser 305 310 315 320 Val Val Leu Ile Thr Gly Ala Gly Gly Met Phe Gly Gly Val Leu Arg 325 330 335 Thr Ser Gly Ile Gly Gly Ala Leu Ala Asp Ser Met Ala Asp Leu Gly 340 345 350 Leu Pro Val Ile Ala Gly Cys Phe Ile Val Ala Ala Val Leu Arg Val 355 360 365 Ala Gin Gly Ser Ala Thr Val Ala Leu Thr Thr Ala Ala Ala Leu Met 370 375 380 Ala Pro Ala Val Ala Ala Ala Asp Phe Asn Glu Phe Gin Leu Ala Ala 385 390 395 400 Ile Val Ile Ser Thr Ala Ala Gly Ser Val Ile Ala Ser His Val Asn 405 410 415 Asp Ser Gly Phe Trp Leu Val Gly Arg Leu Met Asn Ala Asp Val Pro 420 425 430 Thr Thr Leu Lys Thr Trp Thr Val Asn Gin Thr Cys Ile Ala Ile Val 435 440 445 Gly Phe Val Met Ala Tyr Ala Met Phe Gly Leu Ala Ser Leu Ala 450 455 460 <210> 631 <211> 1608 <212> DNA <213> Corynebacterium glutamicum WO 01/00805 <2 <221> CDS <222> (101). (1585) <223> RXN00432 <400> 631 agctaatctg ccttgcttta cacctccggg ctataacaat PCTIIBOO/00926 ccagtgccag tggctaggga tttactaaaa atcgggtaac acgcgcgtag tatttttcgc atg gaa tta ttg gag Met Glu Leu Leu Glu acc Th r ttc Phe gtt Val1 cct Pro aag Lys gcg Al a tgg Trp t cg Se r gg t Gly gcg Ala 150 aa c As n 99c Gl y ttc Phe ttg Le u ca g Gin gcg Ala gcg Ala gg t Gly atg Met act Thr 9gC Gi y 135 gt t Val1 gct Ala acc Thr atc Ile ctc Leu att Ile aag Lys ttc Phe gtt Val1 t gg Trp ctt Le u 120 cc t Pro gt t Val1 ttg Leu cct Pro act Th r gtt Val1 Cgg Arg ga t Asp acg Th r g cg Ala atc Ile 105 gga Gly g cg Ala ttC Ph e ca g Gln tct Ser gat Asp gct Ala atg Met 999 Gly at t Ile c tg Leu att Ile cag Gin tac Tyr ggt Gly tcc Se r 170 acc Thr gtc ValI gc t Ala at t Ile gag Giu tct Ser 75 gcg Ala gcg Ala ttg Le u tac Tyr gt c Val1 155 aa c Asn act Thr att I le 9gc Gly ccg Pro ttc Ph e 60 gcg Ala atc Ile c tg Le u t gg Trp a tg Met 140 gcc Ala g cg Ala gc a Ala aat Asn ctc Leu gag Glu 45 gcg Ala g cg Ala act Thr gt t Val aag Ly s 125 a cg Thr atc Ile gt t Val1 aag Lys gac aat Asp Asn 15 tat ttc Tyr Phe 30 atg ttc Met Phe gac aag Asp Lys tcg cga Ser Arg ctg ggt Leu Gly 95 ggc ggt Gly Gly 110 gtg aag Val Lys ctt ggt Leu Gly acg ttg Thr Leu gtt gag Val Glu 175 gct ttt Ala Phe 190 ggc ggc ttg Le u 9gt Gly aaa Lys cag Gin gt t Val1 80 gga Gly gcg Ala ga c Asp ttg Leu acc Th r 160 gcg Ala gt t Val gtg 1 tgg Trp 999 Gly gcg Ala gac Asp ggt Gly ccg Pro aca Th r ggc Gly aat As n 145 ttt Phe att Ile Gly cag a tg Met cg t Arg gte Val1 att Ile a cg Thr gg t Gly t cg Se r ga c Asp 130 get Ala ggt Gly a cg Thr ttg Le u eg t ate ttg Ile Leu acg ttg Thr Leu gtc gag Val Glu tcg gct Ser Ala gcg aat Ala Asn gca gtg Ala Val 100 ttc att Phe Ile 115 agc tat Ser Tyr cgg tgg Arg Trp ttt gtg Phe Val gta tca Val Ser 180 ggc atg Gly Met 195 atc gca ccc Pro ctg Leu aag Lys ttt Phe gtt Val1 ttc Phe gag Glu eg c Arg ctt Le u tac T yr 165 ctc Le u gct Ala aac 115 163 211 259 307 355 403 451 499 547 595 643 691 739 185 gga ttg tct gcg ctg gtt att ttt WO 01/00805 WO 0100805PCT[IBOO/00926 Gly Leu Ser Ala Leu Val Ile Phe Gly Gly Val Gin gtg Val1 gg t Gly 230 aac Asn t cg Ser gga Gly gct Ala ctq Leu 310 gtc Val1 tct Ser acc Thr c tg Leu t cg Ser 390 ttc Phe act Thr ctg Leu a cg Thr 215 gtg Val1 gac Asp gtg ValI ci g Leu acc Tb r 295 ggc Gi y atc Ile tct Ser cac His gg t Gi y 375 aag Lys tct Se r ttc Phe gg t Gi y 200 ca g Gin gtg ValI atc Ile tgg Trp ttc Ph e 280 gcg Ala gt a Val ctg Le u ttg Le u ttc Ph e 360 aac Asn act Thr ggc Gly qct Ala ggC Gly 205 tgg Trp gtg Val att Ile gg C Gly 265 tcC Ser act Thr tat Tyr ctg Le u act Th r 345 att Ile tac Tyr gt c Val1 gcg Aila ggC Giy 425 gt t Va I atg Met at Ile gct Ala 250 g cg Ala aat As n gtg Val1 ttc Phe tct Ser 330 cag Gin a cc Thr tac T yr atg Met gt Vali 410 atc Ile gca Ala gitt Vali gt g Vai 235 ggt Gly ttc Phe gag Giu Sc t Se r gac Asp 315 gg a Giy icc Se r gt a Val1 ttg Le u act Th r 395 gci Ala a tg Met gtq ValI ccg Pro 220 att Ile gct Ala t gg Trp gct Ala cac His 300 a cc Thr gtg Val1 g cg Ala gtg Val1 g ca Al a 380 gitt Val1 t cg Se r gtg Val aag Lys ttc Phe aac As n tt Phe ttg Leu gg t Gly 285 cct Pro ct g Leu gag Giu ct g Leu atg Met 365 cag Gin ttc Phe gig Val1 ctc Le u t tg Leu atg Met att Ile ggt Gly g cg Ala 270 gag Giu gig Val ctc Le u tac Tyr gct Ala 350 ttc Phe g cg Ala cga Arg ccg Pro att Ile 430 ct Le u g cg Ala cag Gin ttc Phe 255 ttt Phe ggt Gly aag Lys gt ValI gcg Ala 335 agc Ser ttc Ph e aat Asn ctc Leu ttg Le u 415 aac Asn a ag Lys gg t Gly cag Gin 240 cg t Arg at g Met i ct Se r cag Gin tgt Cys 320 acc Tb r gtt Val1 ci g Le u att Ilie tt g Le u 400 atc Ile ct g Le u aat As n g cg Ala 225 gtt Val ccg Pro aac As n gtc Val1 ggi Gly 305 a gc Ser ggc Gly gt i Val1 g cg Ala ca g Gin 385 gt g Val tgg Trp gcq Ala tac Tyr Arg 210 tac Tyr ccg Pro gi i Val ggt Gly ccg Pro 290 ttg Leu att Ile gati Asp gg t Gly ttt Phe 370 tac Tyr cig Le u gci Ala gc g Al a acc Tb r aic Ile acc Thr gct Ala atg Met 275 aac As n gt i Val1 acc Thr att Ile ggt Gly 355 tct Ser t tc Ph e cic Leu i tg Le u atc Ile 435 att Ile Ile Ala Asn ati Ile a tg Met act Thr 260 cgc Arg gct Ala cag Gin gci Ala cag Gin 340 t gg T rp tcc Ser acc Th r agc Ser gg i Gly 420 ati Ile cag Gin gtg Val1 atc Ile 245 gcg Ala cg t Arg g ct Ala act Thr ttt Phe 325 tct Ser gga Gi y gt g Val1 ga t Asp gtg Val 405 ga t Asp ccg Pro aag Lys 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 WO 01/00805 440 445 aag gct ggt ctqgqat cct gtg ttc cac Lys Ala Gly Leu Asp Pro Val Phe His 455 460 cgt aat att gcg tgc tgg aac ggc aaa Arg Asn Ile Ala Cys Trp Asn Gly Lys 470 475 cac gaa gcg atg gaa gtg atc aag aag His Glu Ala Met Glu Val Ile Lys Lys 490 gta <210> 632 <211> 495 <212> PRT <213> Corynebacterium glutamicum <400> 632 Met Giu Leu Leu Giu Thr Phe Ile Thr PCT/IB00100926 450 cgc gac atg atg cca gag gtt Arg Asp Met Met Pro Glu Val 465 gat gca gct aca tcc aac tat Asp Ala Ala Thr Ser Asn Tyr 480 485 agc tagtcatcga aggaacagtg Se r 495 1507 1555 1605 1608 1 Trp Giy Ala Asp Gly Pro Th r Gly As n 145 Ph e Ile Gly Met Arg Val Ile Th r Gly Ser Asp 130 Ala Gly Thr Le u Ile Thr Val Ser Ala Ala Phe 115 Ser Arg Phe Val1 Gl y 195 Le u Leu Giu Ala As n Val 100 Ile Tyr Trp Val Se r 180 Met 5 Pro Leu Lys Ph e Val Phe Giu Arg Leu Tyr 165 Leu Ala Phe ValI Pro L ys 70 Ala Trp Ser Gly Ala 150 As n Gly Gi y Le u Gin Ala 55 Ala GI y Met Thr Gly 135 Val1 Ala Th r Le u Le u Ile 40 Lys Phe Val1 Trp Le u 120 Pro Val Le u Pro Ser 200 Val 25 Arg Asp Th r Ala Ile 105 Gly Ala Phe Gin Ser 185 Ala Asp 10 Ala Met Gly Ile Le u 90 Ile Gin Tyr GI y Ser 170 Th r Le u ValI Ala Ile Giu Ser 75 Ala Ala Leu Tyr Val 155 Asn Thr Val Ile Gi y Pro Ph e Ala Ile Leu Trp Met 140 Ala Ala Ala Ile Asn Leu Giu Ala Ala Thr Val1 L ys 125 Thr Ile ValI Lys Ph e 205 Asp T yr Met Asp Ser Le u Gly 110 Val Le u Thr Val Ala 190 Gly Asn Phe Phe Lys Arg Gly Gly Lys Gly Leu Giu 175 Phe Gly Le u Gly Lys Gin Val Gly Ala Asp Leu Thr 160 Ala ValI Val1 WO 01/00805 PCT/IB00/00926 Gin Arg Ile Ala Asn Val Thr Gin Trp Met Val Pro Phe Met Ala Gly 210 215 220 Ala Tyr Ile Ile Val Gly Val Val Val Ile Val Ile Asn Ile Gin Gin 225 230 235 240 Val Pro Thr Met Ile Asn Asp Ile Ile Ala Gly Ala Phe Gly Phe Arg 245 250 255 Pro Val Ala Thr Ala Ser Val Trp Gly Ala Phe Trp Leu Ala Phe Met 260 265 270 Asn Gly Met Arg Arg Gly Leu Phe Ser Asn Glu Ala Gly Glu Gly Ser 275 280 285 Val Pro Asn Ala Ala Ala Thr Ala Thr Val Ser His Pro Val Lys Gin 290 295 300 Gly Leu Val Gin Thr Leu Gly Val Tyr Phe Asp Thr Leu Leu Val Cys 305 310 315 320 Ser Ile Thr Ala Phe Val Ile Leu Leu Ser Gly Val Glu Tyr Ala Thr 325 330 335 Gly Asp Ile Gin Ser Ser Ser Leu Thr Gin Ser Ala Leu Ala Ser Val 340 345 350 Val Gly Gly Trp Gly Thr His Phe Ile Thr Val Val Met Phe Phe Leu 355 360 365 Ala Phe Ser Ser Val Leu Gly Asn Tyr Tyr Leu Ala Gin Ala Asn Ile 370 375 380 Gin Tyr Phe Thr Asp Ser Lys Thr Val Met Thr Val Phe Arg Leu Leu 385 390 395 400 Val Leu Leu Ser Val Phe Ser Gly Ala Val Ala Ser Val Pro Leu Ile 405 410 415 Trp Ala Leu Gly Asp Thr Phe Ala Gly Ile Met Val Leu Ile Asn Leu 420 425 430 Ala Ala Ile Ile Pro Leu Gly Gly Val Ala Val Lys Leu Leu Lys Asn 435 440 445 Tyr Thr Ile Gin Lys Lys Ala Gly Leu Asp Pro Val Phe His Arg Asp 450 455 460 Met Met Pro Glu Val Arg Asn Ile Ala Cys Trp Asn Gly Lys Asp Ala 465 470 475 480 Ala Thr Ser Asn Tyr His Glu Ala Met Glu Val Ile Lys Lys Ser 485 490 495 <210> 633 <211> 914 <212> DNA <213> Corynebacterium glutamicum WO 01/00805 <~220> <221> COS <222> (891) <223> FRXA00432 PCT/iBOOIOO926 <400> 633 ttg tct gcg Leu Ser Ala 1 acg cag tgg Thr Gin Trp gtg gtg gtg Val Val Val gac atc att Asp Ile Ile gtg tgg ggc Val Trp Gly ctg ttc tcc Leu. Phe Ser acc gcg act Thr Ala Thr ggc gta tat Gly Val Tyr 115 atc ctg ctg Ile Leu Leu 130 tct ttg act Ser Leu Thr 145 cac ttc att His Phe Ile ggt aac tac Gly Asn Tyr aag act gtc Lys Thr Val 195 ctg Le u atg Met att Ile gct Ala g cg Ala aa t As n gtg ValI 100 ttc Phe tct Ser cag Gin acc Thr tac Tyr 180 atg Met gtt Val1 5 gtt Val1 gtg Val1 ggt Gly ttc Phe gag Giu tct Ser gac Asp g ga Gly tcc Se r gta Val 165 ttg Le u act Th r att ttt ggc Ile Phe Gly ggc gtg cag cgt atc gca aac gtg Gly Val Gin Arg Ile Ala Asn Val 10 c cg Pro att Ile gct Ala t gg Trp 70 gct Ala cac His acc Thr gtg Val gcg Ala 150 g tg Val g ca Al a gtt Val ttc Phe aac As n ttt Phe 55 ttg Le u ggt Gly cct Pro ctg Leu gag Giu 135 ct g Le u atg Met ca g Gin ttc Phe atg Met att Ile 40 ggt Gly gcg Ala gag Giu gtg ValI ctc Le u 120 tac T yr gc t Ala ttc Phe gcg Ala cga Arg 200 gcg Al a 25 ca g Gin ttc Ph e ttt Phe gg t Gly aag Lys 105 gtt Val1 g cg Ala agc Se r ttc Phe aat Asn 185 ct c Le u ggt Gly cag Gin cgt Arg a tg Met tct Se r 90 cag Gin tgt Cys acc Thr gtt Val1 ctg Le u 170 att Ile ttg Le u atc Ile gcg tac Ala Tyr gtt ccg Val Pro ccg gtt Pro Val aac ggt Asn Gly 75 gtc ccg Val Pro ggt ttg Gly Leu agc att Ser Ile ggc gat Gly Asp 140 gtt ggt Val Giy 155 gcg ttt Ala Phe cag tac Gin Tyr gtg ctg Val Leu tgg gct Trp Ala 220 atc att Ile Ile acc atg Thr Met gct act Ala Thr atg cgc Met Arg aac *gct Asn Aia gtt cag Val Gin 110 acc gct Thr Ala 125 att cag Ile Gin ggt tgg Gly Trp tct tcc Ser Ser ttc acc Phe Thr 190 ctc agc Leu Ser 205 ttg ggt Leu Gly gtg Val atc Ile gcg Ala cg t Arg gct Ala act Thr ttt Ph e tct Ser gga Gly gt g Val1 175 ga t Asp gtg Val1 ga t Asp ggt Gly aac As n tcg Ser gga Giy gct Ala ctg Le u gtc Val1 t ct Ser acc Thr 160 ctg Le u t cg Se r ttc Phe act Th r 96 144 192 240 288 336 384 432 480 528 576 624 672 tct ggc gcg gtt gct tcg gtg ccg ttg Ser Gly Ala Val Aia Ser Val Pro Leu 210 215 WO 01/00805 ttc gct ggc atc atg gtg ctc att aac Phe Ala Gly Ile Met Val Leu Ile Asn 225 230 ggt ggc qtt gca gtg aag ttg ctt aag Gly Gly Val Ala Val Lys Leu Leu Lys 245 gct ggt ctg gat cct gtg ttc cac cgc Ala Gly Leu Asp Pro Val Phe His Arg 260 265 aat att gcg tgc tgq aac ggc aaa qat Asn Ile Ala Cys Trp Asn Gly Lys Asp 275 280 gaa gcg atg gaa gtg atc aag aag agc Glu Ala Met Giu Val Ile Lys Lys Ser 290 295 g ta <210> 634 (211> 297 (212> PRT <213> Corynebacterium glutamicum <400> 634 Leu Ser Ala Leu Val Ilie Phe Gly Gly ctg gcg gcg atc att ccg Leu Ala Ala Ile Ile Pro 235 aat tac acc att cag aag Asn Tyr Thr Ile Gin Lys 250 255 gac atg atg cca gag gtt Asp Met Met Pro Glu Val 270 gca gct aca tcc aac tat Ala Ala Thr Ser Asn Tyr 285 tagtcatcga aggaacagtg PCT/1BOO/00926 ctg 720 Le u 240 aag '768 Lys cgt 816 Arg cac 864 His 911 1 Thr Val Asp ValI Le u Thr Gly Ile Se r 145 His Pro Ile Ala Trp 70 Ala His Thr Val1 Ala 150 Val1 Phe As n Phe Le u Gly Pro Le u Giu 135 Le u Met Met Ile Gly Ala Giu Val1 Le u 120 T yr Ala Phe Ala Gln Ph e Phe Gly Lys 105 ValI Al a Se r Phe Val1 10 Gly Gin Ar g Met Ser Gin Cys Thr Val1 Le u Gin Ala Val1 Pro Asn 75 Val1 Gly Se r Gly ValI 155 Ala Arg Tyr Pro Vali Gly Pro Leu Ile Asp 140 Gly Phe Ile Ile Th r Ala Met As n Val Th r 125 Ile Gly Se r Ala Ile Met Th r Arg Al a Gin 110 Ala Gin Trp Se r Asn Val Ile Ala Arg Ala Thr Phe Se r Gly Val1 Val Giy Asn Se r Gly Ala Le u Val1 Ser Thr 160 Le u WO 01/00805 WO 0100805PCTIBOO/00926 165 170 175 Gly Asn Tyr Tyr Leu Ala Gin Ala Asn Ile Gin Tyr Phe Thr Asp Ser 180 185 190 Lys Thr Val Met Thr Val Phe Arg Leu Leu Val Leu Leu Ser Val Phe 195 200 205 Ser Gly Ala Val Ala Ser Val Pro Leu Ile Trp Ala Leu Gly Asp Thr 210 215 220 Phe Ala Gly Ile Met Val Leu Ile Asn Leu Ala Ala Ile Ile Pro Leu 225 230 235 240 Gly Gly Vai Ala Val Lys Leu Leu Lys Asn Tyr Thr Ile Gin Lys Lys 245 250 255 Ala Gly Leu Asp Pro Val Phe His Arg Asp Met Met Pro Giu Val Arg 260 265 270 Asn Ilie Ala Cys Trp Asn Gly Lys Asp Ala Ala Thr Ser Asn Tyr His 275 280 285 Giu Ala Met Giu Val Ilie Lys Lys Ser 290 295 <210> 635 <211> 569 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (45)..(569) <223> FRXA00436 <400> 635 gqgatttact aaaaatcggg taacacgcgc gtagtatttt tcgcatg gaa tta ttg 56 Met Giu Leu Leu 1 gag acc ttc atc act gat gtc att aat qac aat ttg tgg atg atc ttg 104 Giu Thr Phe Ilie Thr Asp Val Ile Asn Asp Asn Leu Trp Met Ile Leu 10 15 ccc ttc ttg ctc gtt gct gct ggc ctc tat ttc ggt ggg cgt acg ttg 152 Pro Phe Leu Leu Val Ala Ala Gly Leu Tyr Phe Gly Gly Arg Thr Leu 30 ctq gtt cag att cgg atg att ccq gag atg ttc aaa gcg gtc gtc gag 200 Leu Val Gin Ile Arg Met Ile Pro Giu Met Phe Lys Ala Val Val Giu 45 aag cct gcg aag gat ggg gag ttc gcg gac aag cag gac att tcg gct 248 Lys Pro Ala Lys Asp Giy Giu Phe Ala Asp Lys Gin Asp Ile Ser Ala 60 ttt aag gcg ttc acg att tct gcg gcg tcg cga gtt gqt acg gcg aat 296 Phe Lys Ala Phe Thr Ilie Ser Ala Ala Ser Arg Val Gly Thr Ala Asn 75 WO 01/00805 WO 0100805PCT/IBOO/00926 gtt gcg ggt gtt gcg ctg gcg atc act ctg ggt gga ccqgqgt gca gtg 344 Val Ala Gly Val Ala. Leu Ala Ile T1hr Leu Gly Gly Pro Gly Ala Val 90 95 100 ttc tgg atg tgg atc att gcg ctg gtt ggc ggt gcg aca tcg ttc att 392 Phe Trp Met Trp Ile Ile Ala Leu Val Gly Gly Ala Thr Ser Phe Ile 105 110 115 gag tcg act ctt gga cag ttg tgg aag gtg aag gac ggc gac agc tat 440 Giu Ser Thr Leu Gly Gin Leu Trp Lys Val Lys Asp Gly Asp Ser Tyr 120 125 130 cgc ggt ggc cct gcg tac tac atg acg ctt ggt ttg aat gct cgg tgg 488 Arg Giy Giy Pro Ala Tyr Tyr Met Thr Leu Gly Leu Asn Ala Arg Trp 135 140 145 ctt gcg gtt gtt ttc ggt gtc gcc atc acg ttg acc ttt ggt ttt gtg 536 Leu Ala Val Val Phe Gly Val Ala Ile Thr Leu Thr Phe Gly Phe Val 150 155 160 tac aac gct ttg cag tcc aac gcg .gtt gtt gag 569 Tyr Asn Ala Leu Gin Ser Asn Ala Val Val Glu 165 .170 175 (210> 636 <211> 175 <212> PRT <213> Corynebacterium giutamicum <400> 636 Met Giu Leu Leu Glu Thr Phe Ile Thr Asp Vai Ile Asn Asp Asn Leu 1 5 10 Trp Met Ile Leu Pro Phe Leu Leu Val Ala Ala Gly Leu. Tyr Phe Giy 25 Gly Arg Thr Leu Leu Val Gin Ile Arg Met Ile Pro Glu Met Phe Lys 40 Ala Val Val Glu Lys Pro Ala Lys Asp Gly Glu Phe Ala Asp Lys Gin 55 Asp Ilie Ser Ala Phe Lys Ala Phe Thr Ile Ser Ala Ala Ser Arg Vai 70 75 Gly Thr Ala Asn Val Ala Gly Vai Ala Leu Ala Ile Thr Leu Gly Giy 90 Pro Gly Ala Val Phe Trp Met Trp Ilie Ilie Ala Leu Val Gly Gly Ala 100 105 110 Thr Ser Phe Ilie Giu Ser Thr Leu Gly Gin Leu Trp Lys Val Lys Asp 115 120 125 Gly Asp Ser Tyr Arg Gly Gly Pro Ala Tyr Tyr Met Thr Leu Gly Leu 130 135 140 Asn Ala Arg Trp Leu Ala Val Val Phe Gly Val Ala Ile Thr Leu Thr 145 150 155 160 WO 01/00805 Phe Gly Phe Val Tyr Asn Ala Leu Gin Ser Asn Ala Val Val Glu 165 170 175 PCTIBOOIOO926 <210> 637 <211> 1572 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (101)..(1549) <223> RXA00847 (400> 637 tctggcccgc tgtgtcctct ggacgcagcg ggccaqtagt attttggggg tgagaataaq gtttggaatt ctaagtgttg tcgcctgcac ggtttcgttg gtg gca tgt cag gct aa t Asn acc Thr ga t Asp aat Asn t cg Se r ttg Leu att Ile ga t Asp aac Asn gga Gly 150 ggt Gi y gca Ala gaa Giu cat His gtg Val1 qa c Asp ca g Gin gag Glu 120 tct Ser act Thr cc t Pro act Thr acc Th r ttg Le u ttg Leu aa t As n gaa Giu 105 tac Tyr gca Ala gtc Val g cg Ala gag Giu atc Ile t tg Le u ccg Pro gt t Val g cg Ala t tg Le u tat T yr gac Asp ccc Pro a cg Th r agt Se r tct Se r agt Ser 75 gag G lu cag Gin cg c Arg t cg Se r gtt Val 155 gag Glu gt a Val1 30 atc Ile t ct Se r gag Giu ga t Asp ga t Asp 110 att Ile tca Se r gaa Glu cca Pro 15 gag Giu ga t Asp cca Pro ggc Gly gaa Giu 95 ggc Gly gtg ValI gag Giu cat His Val 1 aca Thr ga a G iu atc Ile gtg Val cag Gin a cg Thr c cg Pro ggg Gi y agg Arg 145 gt c ValI act Thr ccc Pro aac As n ga t Asp aac As n agg Arg act Th r 115 gga Gly t ca Ser gat Asp Ala Cys Gin Ala g cg Ala aaa Lys ggt Giy act Thr act Thr tac Tyr 100 ggg Gly a cg Thr ggc Gly t gg Trp acg Th r caa Gin ttc Phe gca Ala ga c Asp acg Th r tct Se r ct g Le u ggt Gly cat His 165 115 163 211 259 307 355 403 451 499 547 595 WO 01/00805 WO 0100805PCTIBOO/00926 ttg ctg ttt aac aat ttg ttg ccg agt cat ttq atc act ggc aat tcc 643 Leu Leu Phe Asn Asn Leu Leu Pro Ser His Leu Ile Thr Giy Asn Ser 170 175 180 aca ttc cag aca gcg ttt tat gat tcc atc ccg gcc tct. gcg ggg cgc 691 Thr Phe Gin Thr Ala Phe Tyr Asp Ser Ile Pro Ala Ser Ala Gly Arg 185 190 195 tac atg gtq cgt tcc att gat cgt cag cgc ggc gtg att acg ttq tcg 739 Tyr Met Val Arg Ser Ile Asp Arg Gin Arg Gly Val Ile Thr Leu Ser 200 205 210 cgc aat gat cgt ttc tgg ggt gca aat ccg gca cac gtg gaa gta ctc 787 Arg Asn Asp Arq Phe Trp Giy Aia Asn Pro Ala His Vai Giu Val Leu 215 220 225 caa ttc aac acc gtt gcc tct gca tcc cgg gcg ggg gag tac ctg cgc 835 Gin Phe Asn Thr Val Ala Ser Ala Ser Arg Ala Gly Glu Tyr Leu Arg 230 235 240 245 acc ggg cag agt tcg ttt atg aat ctg tca ccg cag gaa acc ctg gtg 883 Thr Gly Gin Ser Ser Phe Met Asn Leu Ser Pro Gin Glu Thr Leu Val 250 255 260 gac acg ttg aat ttg gtg ccg gac acg gaa gtg cgg gtg tct gac acg 931 Asp Thr Leu Asn Leu Val Pro Asp Thr Giu Val Arg Val Ser Asp Thr 265 270 275 acg cgc acg ctq gag ctg gta ttc aac gcc gaa gcg ctg gca ccg gcg 979 Thr Arg Thr Leu Giu Leu Val Phe Asn Ala Glu Ala Leu Ala Pro Ala 280 285 290 cag cgc gcc tac ctg aca tcg ctt atc gac gtc ccc ctc acc gcc aag 1027 Gin Arg Aia Tyr Leu Thr Ser Leu Ile Asp Val Pro Leu Thr Ala Lys 295 300 305 tta gct ggc ggt cgc agt gca aac ctg ggt gtg ccc caa acg gtg gag 1075 Leu Ala Gly Gly Arg Ser Ala Asn Leu Gly Val Pro Gin Thr Val Giu 310 315 320 325 gcg agc gtc gat aag caa gaa att cct gcg ttg cgc *ctg gcg gcg gac 1123 Ala Ser Val Asp Lys Gin Glu Ile Pro Ala Leu Arg Leu Ala Ala Asp 330 335 340 ccc gcc gac gat gct ggt ttg gca gct gcg cgg ggc atc gtc gat atg 1171 Pro Ala Asp Asp Ala Gly Leu Ala Ala Ala Arg Gly Ile Val Asp Met 345 350 355 ctg gct gcg gat gga atc aag gct cag gtc gtg aca acc gat ttg aat 1219 Leu Aia Ala Asp Gly Ile Lys Ala Gin Val Val Thr Thr Asp Leu Asn 360 365 370 tcg gcg att gcg ggt aat ttt gat gcg atc gtg gcg tgg acc aga act 1267 Ser Ala Ile Ala Gly Asn Phe Asp Aia Ile Val Ala Trp Thr Arg Thr 375 380 385 gcc acg gat tcg att gcg ttg gcg gac cga gtg ggt tgt ggc gtg aac 1315 Ala Thr Asp Ser Ile Ala Leu Ala Asp Arg Val Gly Cys Gly Vai Asn 390 395 400 405 WO 01/00805 ttg gca aag tgg tgc gct gag gga z Leu Ala Lys Trp Cys Ala Giu Gly 410 ttg gcg ggt gag att gat ttc gat c Leu Ala Gly Giu Ile Asp Phe Asp 4251 acc gag aat cac ttg cgg gtt ccg E Thr Giu Asn His Leu Arg Val Pro 440 445 gcg aaa aat aac ggt atc ctc ggg Ala Lys Asn Asn Gly Ile Leu Gly .1 455 460 att tca agt gct gca agt tgg agg Ile Ser Ser Ala Ala Ser Trp Arg 1 470 475 tgatctgtct ggtgctcgag ttg <210> 638 <211> 483 <212> PRT <213> Corynebacteriun glutamicun a cg Th r 415 g cg Ala ctg Leu gcg Ala aac Asn gaa Glu t gg Trp cgg Arg ga t Asp ga t Asp 480 tat Tyr.

gag Giu gag G iu Gly 4 gt t Val PCTIBOOIOO926 gtt 1363 ValI aac 1411 As n gaa 1459 Glu gga 1507 Gly 1549 1572 <400> 638 Val Ala Cys Gin Ala Asn Pro Gly Pro Ala Pro Vai Glu Giu Pro Thr 1 Thr Glu Ile Val Gin Thr Pro Gly Arg 145 ValI Al a Lys Gly Thr Thr Tyr 100 Gi y Thr Gly Trp Th r Asp As n Ser 70 Le u Ile Asp Asn Gly 150 Leu Thr Arg Pro* Le u Le u Ala Ph e Asp 135 Lys Le u Ala Glu His Val1 Asp Gin Glu 120 Ser Thr Phe Glu Ile Le u Pro Val1 Ala Le u T yr Asp Asn 170 Th r Se r Se r Se r 75 Gi u Gin Arg Ser Val1 155 Le u Th r Ile Asp Ala Gin Trp Arg Ala 140 Ile Le u Thr Gly Asp Phe ValI Ser Ser 125 Ile Phe Pro Val1 Ile Se r Glu Asp Asp 110 Ile Ser Gi u Ser Glu Asp Pro Gly Giu Gly Val1 Glu His His 175 Thr Pro Le u As n Thr Thr Al a Ile Pro 160 Le u Ile Ala Val1 His 225 Giy Gin Arg Aia Pro 305 Pro Arg Gly Thr Aia 385 Gly T yr Giu Giu Giy WO 01/00805 Thr Giy Asn 180 Ser Ala Giy 195 Ile Thr Leu 210 Vai Giu Vai Giu Tyr Leu Giu Thr.Leu 260 Vai Ser Asp 275 Leu Aia Pro 290 Leu Thr Aia Gin Thr Val Leu Aia Ala 340 Ile Val Asp 355 Thr Asp Leu 370 Trp Thr Arg Cys Giy Vai Ile Asn Giy 420 Gin Gin Phe 435 Thr Arg Vai 450 Trp Pro Giy PCT/IBOO/00926 Ser Arg Se r Leu Ar g 245 Vali Thr Ala Lys G iu 325 Asp Met As n Thr As n 405 Va 1 Asn Giu Thr Phe Tyr Met Arg Asn 21.5 Gin Phe 230 Thr Gly Asp Thr Thr Arg Gin Arg 295 Leu Aia 310 Ala Ser Pro Ala Leu Aia Ser Aia 375 Aia Thr 390 Leu Aia Leu Ala Thr Giu Ala Lys 455 Gin Vali 200 Asp As n Gin Le u Th r 280 Ala Giy Val Asp Ala 360 Ile Asp Lys Gly Asn 440 As n Th r 185 Arg Arg Th r Ser Asn 265 Le u Tyr Giy Asp Asp 345 Asp Ala Ser T rp Giu 425 His As n Aia Se r Phe Vali Se r 250 Le u Giu Le u Arg Lys 330 Ala Ci y Gly Ile Cys 410 Ile Le u Gi y Phe Ilie Trp Aila 235 Phe Val1 Le u Thr Ser 315 Gin Gly Ile Asn Ala 395 Ala Asp Arg Ile T yr Asp Giy 220 Se r Met Pro Val1 Se r 300 Ala Giu Le u L ys Phe 380 Le u Giu Phe ValI Leu 460 Asp Arg 205 Ala Ala As n Asp Phe 285 Leu As n Tie Ala Aila 365 Asp Aia Gly Asp Pro 445 Giy Se r 190 Gin Asn Se r Le u Th r 270 As n Ile Le u Pro Ala 350 Gin Ala Asp Th r Pro 430 Ile Ala Ile Arg Pro Arg Ser 255 Glu Ala Asp Gly Ala 335 Ala Val Ile Arg Thr 415 Ala Leu Ala Pro Ci y Ala Ala 240 Pro Val1 Giu Val1 Val1 320 Le u Arq Val Val Val1 400 Giu Trp Arq Asp Gly Ile Ser Ser Ala Ala Ser Trp Arg Lys Asn Asp Val Giu Glu WO 01/00805 <210> 639 <211> 1192 (212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (1192) <223> RXN01382 <400> 639 aaaggatgta cctcatgagc accacqatta cicgccqcaa PCT/lBOO/00926 tttcctccga gcaaccggaa tcctcggtgt cgcagctggc atcggcgcaa cacttgctgc aig igc acc aac aac Met Cys Thr Asn Asn acc Thr aat Asn aac Asn aac As n gag Glu t cc Ser g ca Ala cca Pro gic Val1 acc Thr 150 icc Ser ggc Gly gaa Glu ggc Gly t gg Trp gtc Val1 ct a Leu gtc ValI g cc Ala acc Th r 135 ggc Gly gtc Val1 acc Thr ga a Glu tac Tyr cac His tac Tyr ga c Asp acc Thr aac As n 120 aag Lys atc Ile gig Val agc Ser ggc Gly ga c Asp acc Th r gca Ala aic Ile gca Ala 105 aac Asn aag Lys atc Ile gaa Gi u gga Gly acc Th r cca Pro ct Le u gca Ala aag Lys ga c Asp t cc Se r gat Asp agc Se r gca Ala 170 icc Se r atc Ile atg Met gaa Glu ctc Le u 75 ct Le u gac Asp ctt Leu gac Asp gaa Glu 155 qac Asp acc Thr a cc Thr acc Thr ggc Gly 60 gcc Al a cgc Arg gia Val tac Tyr a cc Thr 140 cgc Arg gca Ala icc Se r g ca Al a acc Thr 45 ct c Le u agc Ser gac Asp gt c Val g ca Ala 125 acc Thr ctc Le u icc Se r acc Th r gcc Ala 30 acc Thr a cc Thr gca Ala 9gc Gly ttc Phe 110 icc Se r gt c Val1 gca Ala gga Gly gca Ala 15 atc Ile tcg Se r ga a Glu ct g Le u g cc Ala 95 tct Se r tic Ph e acc Thr gi a ValI tic Phe 175 1 gg C Gly tac Tyr ctc Leu gac Asp t ca Ser tic Phe gag Glu cca Pro gac Asp 145 aag Lys gcc Ala acc Thr gaa Glu act Thr cca Pro gcc Ala cac His cga Arg tc Phe 130 ci c Leu aic Ile aa C Asn gga Gly ctg Leu gig ValI gcc Ala ga t Asp aac As n gic Val1 115 atc Ile ga c Asp gig Val1 cca Pro act Thr gga Gly g ca Ala acc Th r gcg Ala ggc Gly 100 cic Le u aag Lys tac Tyr cca Pro atc Ile 180 gca Ala acc Thr gc C Ala ggc Gly a cc Thr gac Asp gati Asp t ca Se r gca Ala a ag Lys 165 ggc Gly 115 163 211 259 307 355 403 451 499 547 595 643 WO 01/00805 WO 0100805PCT/IBOO/00926 tcc ggc Ser Gly ttc gag Phe Giu aag atg Lys Met 215 ctt cag Leu Gin 230 atc cca Ilie Pro ggc ctc Giy Leu gac gtc Asp Val atc gtt Ilie Val 295 gtg caq Val Gin 310 ctc gac Leu Asp aqc ctc Ser Leu cca ctg Pro Leu cca tac aag atg acc Pro Tyr Lys Met Thr 185 cgc aac gac gac tac Arg Asn Asp Asp Tyr 200 gaa tgg cag atc atc Giu Trp Gin Ile Ile 220 tcc ggc agc acc atg Ser Giy Ser Thr Met 235 cag ctt gaa gcc aCC Gin Leu Giu Ala Thr 250 ctq ttc gcc atg ttc Leu Phe Aia Met Phe 265 cgc aac cgc cag gca Arg Asn Arg Gin Aia 280 aag acc gqc atg tcc Lys Thr Giy Met Ser 300 aag gaa cac ccc aac Lys Giu His Pro Asn 315 gcc gac aaa gcc aag Aia Asp Lys Aia Lys 330 aac ctc ctg tgc acc Asn Leu Leu Cys Thr 345 atc cag gaa tcc ctc Ile Gin Giu Ser Leu 360 gac aac ggt gca Asp Asn Giy Aia tcc aag gtg gtc aag Ser Lys Val Vai Lys 190 195 aac As n 205 ccc Pro gcc Aia a gc Se r agc Ser ttc Phe 285 gac Asp t ac Tyr gcg Aia gac Asp ggc Gi y ga c Asp atc Ile acc Th r tqc Cys 270 ctc Le u cag Gin aac Asn ctg Le u cac His 350 cca Pro g cc Ala ga c Asp gta Vali 255 t cc Se r tac T yr gca Aia cag Gin ttc Phe 335 ga c Asp cg C Arg t cc Se r tcc Se r 240 gaa Giu gaa Giu gca Aila acc Thr gca Aila 320 gct Aia t gg Trp c ca Pro a cc Th r 225 gt t Vali tcc Se r qg C Giy ct g Leu cca Pro 305 tcc Ser gaa Giu gtc Val g ca Aila 210 cg C Arg cca Pro gtc Vali aac As n ga C Asp 290 gcc Aila a cg Thr a CC Th r aag Lys cgt gcc Arg Ala acc aac Thr Asn tac ctg Tyr Leu caa gga Gin Giy 260 CCC ttc Pro Phe 275 atg gac Met Asp acc tcc Thr Ser gtc tac Vai Tyr ggc ctt Gly Leu 340 aac tgc Asn Cys 355 gcC Ala t CC Se r tcg Se r 245 ttc Phe aac Asn aag Lys ttc Phe tcc Se r 325 acc Thr acc Th r 739 787 835 883 931 979 1027 1075 1123 1171 1192 <210> 640 <211> 364 <212> PRT <213> Corynebacterium glutarnicum <400> 640 Met Cys Thr Asn Asn Thr Giy Thr Ser Gly Ser Thr Ser Thr Ala Ala 1 5 10 Giy Thr Giy Thr Ala Asn Glu Giu Gly Thr Ilie Thr Ala Ala Ile Ser 25 Tyr Leu Asp Ser Phe Glu Pro Asp 145 Lys Ala Ser Pro Thr 225 Val Ser Gly Leu Pro 305 Ser WO 01/00805 Glu Leu Gly Thr Val Ala Pro Ala Thr Ala Asp Ala His Asn Gly 100 Arg Val Leu 115 Phe Ile Lys 130 Leu Asp Tyr Ile Val Pro Asn Pro Ile 180 Lys Val Val 195 Ala Arg Ala 210 Arg Thr Asn Pro Tyr Leu Val Gin Gly 260 Asn Pro Phe 275 Asp Met Asp 290 Ala Thr Ser Thr Val Tyr PCT/IB00/00926 Thr Ala Gly Thr Asp Asp Ser Ala Lys 165 Gly Lys Ala Ser Ser 245 Phe Asn Lys Phe Ser 325 Asn Asn Glu 70 Ser Ala Pro Val Thr 150 Ser Ser Phe Lys Leu 230 Ile Gly Asp Ile Val 310 Leu Gly Trp 55 Va 1 Leu Val Ala Thr 135 Gly Val Gly Glu Met 215 Gin Pro Leu Val Val 295 Gin Asp Tyr 40 His Tyr Asp Thr Asn 120 Lys Ile Val Pro Arg 200 Glu Ser Gin Leu Arg 280 Lys Lys Ala Asp Thr Ala Ile Ala 105 Asn Lys Ile Glu Tyr 185 Asn Trp Gly Leu Phe 265 Asn Thr Glu Asp Pro Leu Ala Lys 90 Asp Ser Asp Ser Ala 170 Lys Asp Gin Ser Glu 250 Ala Arg Gly His Lys 330 Met Glu Leu 75 Leu Asp Leu Asp Glu 155 Asp Met Asp lie Thr 235 Ala Met Gin Met Pro 315 Ala Thr Gly Ala Arg Val Tyr Thr 140 Arg Ala Thr Tyr Ile 220 Met Thr Phe Ala Ser 300 Asn Lys Thr Leu Ser Asp Val Ala 125 Thr Leu Ser Asp Asn 205 Pro Ala Ser Ser Phe 285 Asp Tyr Ala Thr Thr Ala Gly Phe 110 Ser Val Ala Gly Asn 190 Gly Asp Ile Thr Cys 270 Leu Gin Asn Leu Ser Glu Leu Ala Ser Phe Thr Val Phe 175 Gly Pro Ala Asp Val 255 Ser Tyr Ala Gin Phe 335 Ala Ile Pro Thr Phe Ile Ile Val 160 Asp Ala Arg Ser Ser 240 Glu Glu Ala Thr Ala 320 Ala Glu Thr Gly Leu Thr Ser Leu Asn Leu Leu Cys Thr Asp His Asp Trp 350 WO 01/00805 Vai Lys Asn Cys Thr Pro Leu Ile Gin Giu Ser Leu 355 360 <210> 641 <211> 1062 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1)..(1062) <223> FRXA01382 PCTUBOOIOO926 <400> 641 t cc Se r 1 atc Ile atq Met qaa Glu ctc Le u ctt Leu gac Asp ctt Le u gac Asp gaa Giu 145 gac Asp acc Th r acc Thr acc Th r qgC Gly gcc Aila cq c Arg gt a Val tac Tyr aco Th r 130 cg c Arg gca Al a t cc Se r gca Aia acc Thr ctc Leu aqc Se r qa c Asp qt C Val1 gca Ala 115 acc Th r ctc Le u t cC Ser acc Thr 9C Ala acc Thr acc Th r qca Ala ggC Gi y ttc Phe 100 tcc Ser gtc Val gca Ala gga Gly gca gct Ala Ala 5 atc tcc Ile Ser tcg gct Ser Ala gaa atc Giu Ile ctg cct Leu Pro 70 gcc acc Ala Thr tct ttt Ser Phe ttc atc Phe Ile acc atc Thr Ile gta gta Val Val 150 ttc gac Phe Asp 165 ggc acc gga act gca aat gaa gaa Gly Thr Gly Thr Ala Asn Glu Glu 10 tac Tyr ctc Leu gac Asp 55 t ca Ser t tc Phe gag Giu cca Pro gac Asp 135 aag Lys g cc Ala gaa ctq gga Glu Leu Gly 25 act gtg gca Thr Val Ala 40 cca gcc acc Pro Ala Thr qcc gat gcg Ala Asp Ala cac aac ggc His Asn Gly 90 cqa gtc ctc Arg Val Leu 105 ttc atc aag Phe Ile Lys 120 ctc gac tac Leu Asp Tyr atc gtg cca Ile Vai Pro aac cca atc Asn Pro Ile 170 aag gtg qtc Lys Val Val acc Th r gcc Al a ggC Gly acc Th r 75 ga c Asp ga t Asp tca Ser g ca Al a aag Ly s 155 gqC Gly aaq Ly s aac Asn aac Asn gag Glu t cc Ser gca Ala cca Pro gt c Val1 acc Th r 140 tcc Ser t cc Ser ttc Phe ggC Gly tgg Trp gt c ValI ct a Le u gtc Val1 gcc Ala acc Th r 125 9gC Gly gtc Val ggc Gly gag Giu tac Tyr ca c His tac Tyr gac Asp acc Th r aac Asn 110 aag Lys atc Ile gtq ValI cca Pro cg c Arg ggc acc Gly Thr gac cca Asp Pro acc ctt Thr Leu gca gca Ala Ala atc aag Ile Lys gca gac Ala Asp aac tcc Asn Ser aag gat Lys Asp atc agc Ile Ser gaa qca Glu Ala 160 tac aag Tyr Lys 175 aac gac Asn Asp 96 144 192 240 288 336 384 432 480 528 576 atg acc gac aac Met Thr Asp Asn ggt gca tcc Gly Ala Ser WO 01/00805 WO 0100805PCTIBOOIOO926 qac Asp atc Ile acc Th r 225 9CC Ala atg Met ca g Gin atg Met

CCC

Pro 305 gcc Al a tgC Cys tcc Ser tac Tyr atc Ile 210 atg Met acc Thr ttc Phe gca Aila t CC Ser 290 aac As n aag Lys acc Th r ctc Le u aac Asn 195

CCC

Pro gCC Aia a gc Ser agc Ser ttc Phe 275 gac Asp tac Tyr 9 C9 Aia ga C Asp 99C Gi y gac Asp atc Ile a cc Th r t 9C Cys 260 ctc Leu cag Gin aac Asn Ct 9 Leu cac His 340 C ca Pro 9CC Aia gaC Asp qta ValI 245 tcc Ser taC Tyr gca Aia cag Gin ttc Phe 325 gac Asp Cq C Ar g tCC Se r tcc Ser 230 ga a Glu gaa Glu gca Ala acc Thr g ca Aia 310 gct Al a t 99 Trp C ca Pro acc Th r 215 gt t Vali t CC Ser 9gC Giy ctg Leu CCa Pro 295 t CC Ser qaa Giu qtc Val gca Ala 200 CgC Arg cca Pro gtc Val1 aac Asn gac Asp 280 9CC Ala acg Thr acc Thr aag Lys cgt Arg acc Thr taC Tyr Caa Gin

CCC

Pro 265 atg Met acc Thr gt C Vali 99C Gly a ac As n 345 9CC Ala aac As n Ct 9 Leu gg a Giy 250 ttc Ph e ga C Asp t CC Ser tac Tyr ctt Le u 330 t9C Cys 9CC Al a tCC S er t cg Ser 235 ttc Phe aac Asn aag Lys ttc Phe t CC Ser 315 acc Thr a CC Th r aag Lys Ctt Leu 220 atc Ile 9 9C Gly gaC Asp atc Ile 9 tg Val 300 Ct C Leu agc Se r cca Pro at 9 Met 205 cag Gin CC a Pro Ct C Le u gt c Val qtt Val1 285 cag Gin gaC Asp Ct C Leu Ctg Le u ga a Gl u tcc Ser cag Gln ctg Leu C9C Arg 270 aag Lys aag Lys 9CC Ala aac Asn atc Ile 350 t 99 Trp ggC Gly Ctt Leu ttc Phe 255 aac Asn a CC Thr 9 aa Giu qac Asp ctc Leu 335 Cag Gin cag Gin agc Ser gaa Glu 240 gCC Al a C9 C Arq 99C Gly Cac His aaa Lys 320 Ct 9 Leu gaa Glu 624 672 720 768 816 864 912 960 1008 1056 1062 <210> 642 <211> 354 <212> PRT <213> Corynebacterium glutamicum <400> 642 Ser Thr Ser Thr Ala Ala Giy Thr Giy Thr Ala Asn Glu Giu Gly Thr 1 5 10 Ile Thr Ala Ala Ilie Ser Tyr Glu Leu Giy Thr Asn Gly Tyr Asp Pro 25 Met Thr Thr Thr Ser Ala Leu Thr Val Ala Ala Asn Trp His Thr Leu 40 wo 01/00805 WO 0100805PCT/IBOO/00926 Giu Leu Leu Asp Leu Asp Glu 145 Asp Met Asp Ile Thr 225 Ala Met Gin Met Pro 305 Ala Cys Ser Gly Aila Arq Vai Tyr Thr 130 Arg Ala Thr Tyr Ile 210 Met Th r Phe Ala Ser 290 Asn Ly s Thr Leu Le u Se r Asp Val Ala 115 Thr Le u Se r Asp Asn 195 Pro Ala Ser Ser Phe 275 Asp Tyr Al a Asp Th r Al a Gi y Phe 100 Se r Val Ala Gly As n 180 Gly Asp Ile Thr Cys 260 Le u Gin Asn Le u His 340 Glu Le u Ala Ser Phe Thr Val Phe 165 Gi y Pro Ala Asp Val1 245 Ser Tyr Ala Gin Ph e 325 Asp Ile Pro 70 Thr Phe Ile Ile Val 150 Asp Al a Arq Ser Se r 230 Giu Giu Ala Thr Ala 310 Ala T rp Asp 55 Ser Phe Glu Pro Asp 135 L ys Ala Ser Pro Th r 215 Val1 Ser Gly Le u Pro 295 Ser Giu Val Pro Ala His Arg Phe 120 Leu Ile Asn Lys Ala 200 Arg Pro Val As n Asp 280 Ala Thr Th r Lys Ala Asp Asn Val 105 Ile Asp Val1 Pro Val1 185 Arg Thr Tyr Gin Pro 265 Met Thr Val Gly Asn 345 Th r Alia Gly 90 Leu Lys Tyr Pro Ile 170 Val Ala As n Leu Gly 250 Phe Asp Ser Tyr Le u 330 Cys Giy Th r 75 Asp Asp Se r Ala Lys 155 Gly Lys Ala Ser Ser 235 Phe As n Lys Phe Ser 315 Th r Thr Giu Se r Al a Pro Val1 Th r 140 Ser Ser Phe Lys Le u 220 Ile Gly Asp Ile Val1 300 Le u Ser Pro Val Le u Val Ala Thr 125 Gly Val1 Gly Giu Met 205 Gin Pro Le u Val1 Val 285 Gin Asp Leu Leu Tyr Asp Th r Asn 110 Ly s Ile Val1 Pro A rgc 190 Giu Se r Gin Leu Arg 270 Lys Lys Ala Asn Ile 350 Ala Ile Ala Asn Lys Ile Giu T yr 175 Asn Trp Gly Le u Phe 255 As n Thr Giu Asp Leu 335 Gin Al a Lys A~sp Ser A~sp Ser Ala 160 Lys Asp Gin Ser Giu 240 Ala Arq Giy His Lys 320 Leu Giu WO 01/00805 ':210> 643 ':211> 335 (212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <:222> (312) <:223> RXA02659 <400> 643 ttc ggc aac gac cca gac ctc ctc Phe Gly Asn Asp Pro Asp Leu Leu 1 5 gtc tqq acc gac tcc cgc atg cac Val Trp Thr Asp Ser Arg Met His cag gta caa aac ctc ctg gaa gaa Gin Val Gin Asn Leu Leu Giu Giu 40 gaa cag caa gac atc tgg aac cgc Glu Gin Gin Asp Ile Trp Asn Arg 55 qtt ccc ctc tac ccg ctg ttc cac Val Pro Leu Tyr Pro Leu Phe His 70 tcc aac gcc ctc gtc gac ttc aaq Ser Asn Aia Leu Val Asp Phe Lys ttc tca qgt gtt gca act act gaa Phe Ser Gly Vai Aia Thr Thr Giu 100 <210> 644 <211> 104 <212> PRT <213> Corynebacterium giutamicun <400> 644 Phe Giy Asn Asp Pro Asp Leu Leu 1 5 Vai Trp Thr Asp Ser Arg Met His Gin Vai Gin Asn Leu Leu Giu Giu 40 Giu Gin Gin Asp Ilie Trp Asn Arg 55 Val Pro Leu Tyr Pro Leu Phe His 70 PCTIBOOIOO926 atg cgc tgg tqg tac gcc ggc Met Arg Trp Trp Tyr Ala Giy 10 tgg aag ggc agc gaa tcc tac Trp Lys Gly Ser Giu Ser Tyr 25 ggc atc cgc gct acc gac aaq Gly Ile Arg Ala Thr Asp Lys acc ttc gat gtc atc tcc gac Thr Phe Asp Val Ile Ser Asp cgc aag gtc cca acc gca tgg Arg Lys Val Pro Thr Ala Trp 75 cca atc tca ctc acc ggc ttg Pro Ile Ser Leu Thr Gly Leu 90 taacaacccc agtggctttc aaa qac Asp gac Asp qca Ala aat Asn aac As n aac Asn 48 96 144 192 240 288 Trp Trp Tyr Ala Gly Asp Gly Ser Glu Ser Tyr Asp Arg Ala Thr Asp Lys Ala Asp Val Ile Ser Asp Asn Vai Pro Thr Ala Trp Asn 75 WO 01/00805 PCT/iBOOIOO926 Ser Asn Ala Leu Val Asp Phe Lys Pro Ile Ser Leu Thr Gly Leu Asn 90. Phe Ser Gly Val Ala Thr Thr Glu 100 <210> 645 <211> 933 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(910) <223> RXN02933 <400> 645 tgatctgctg tatcaggtgq ttgatccaaq agtcggtgct ggttccaggg agcgtggctt aagtgacaac gatcaaaaac gttqgggttg ctaqcactaa aaa Lys tct Ser cgc Arg ggt Gly ttg Leu ctg Leu atg Met att Ile atc Ilie ttc Phe ctt Leu cqc Arg gt g Val ggt Gi y cg t Arcj att Ile qt t Val1 ttt Ph e ttg Le u cag Gin 60 qt q Val at g Met atg Met ttc Phe gcc Ala gat Asp a gg Arg atc Ile gcg Ala cgc Arq ttg Leu gg C Gly 125 qt t ValI cc a Pro cac His at g Met atc Ile cgt Arq g cg Ala 110 gcc Ala gt a Val1 qt t Val ct 9 Le u gt t ValI g ca Ala 9 9 C Gly ttc Phe t cc Ser ttt Phe caa Gin ttg Leu qgt Gly gca Al a 80 atg Met cca Pro acg Th r a tg Met 1 gt t Val1 gct Ala gg a Gly tcc Ser tta Le u Val1 gca Ala tgg Trp ctt Leu ttc Phe acg Thr cqc Arg atc Ile qaa Giu ctg Leu tcc Ser 130 ccc ctt tca Pro Leu Ser qct Al a cca Pro ga t Asp qt c ValI Gi y atc Th r ttg Le u 115 gcg Ala q cg Ala cag Gin cg t Arq acg Thr acg Thr ttt Phe 100 ttg Leu atq Met g gg G ly ctg Leu gag Giu tat Tyr t tq Le u cca Pro gtc ValI gcg Al a gtc Val 115 163 211 259 307 355 403 451 499 gcg atc qgc atc gca Ala Ile Gly Ilie Ala 135 qg C Gly atc cct agt ttt Ile Pro Ser Phe cgc gtq gct cgt qca Arg Val Ala Arg Ala WO 01/00805 WO 0100805PCTIIBOO/00926 gqc Gly 150 tca Ser at c Ile atc Ile ccg Pro gg C Gly 230 a cg Thr aca Thr aaa Lys acc Thr ct g Le u ga t Asp 215 qtc ValI gt c Val ttq Le u qta Val1 agc Ser gcg Ala 200 ccc Pro acc Thr ctt Le u qt g Val tcc Ser 170 ttg Le u gcc Ala tgg Trp atg Met ttt Ph e 250 gat ttc Asp Phe gcg ctt Ala Leu 175 gca tca Ala Ser 190 ttc ctc Phe Leu ttg caa Leu Gin tqg CCC Trp Pro qqt gat Giy Asp 255 qca gct gct Ala Ala Ala cat att ttg His Ile Leu qct ttt gcc Ala Phe Ala 195 ttg gg c acc Leu Gly Thr 210 gct caa gca Ala Gin Ala 225 gct gcg atc Ala Ala Ile tta cgc qat Leu Arg Asp cgg cta Arg Leu 165 Ccc aac Pro Asn 180 ctg gcg Leu Ala act ccc Thr Pro tcc atc Ser Ile qct ttg Ala Leu 245 qcc atc Ala Ile gat cca aag cgq gag qtc gqc cgt qCt Asp Pro Lys Arg Giu Vai Gly Arq Ala 265 270 ttq <210> 646 <211> 270 <212> PRT <213> Corynebacteriun glutamicum <400> 646 Met Pro Leu Ser Gly Lys Ile Giy Gly 1 Val Leu Ala Ala Leu Ser Phe Ile Trp 25 Ala Phe Pro Gin Giu Arg Leu Glu Gly 40 Gly Thr Asp Arg Tyr Gly Arg Asp Val Ser Arg Val Thr Leu Leu Val Gly Ile 70 Leu Ile Gly Thr Pro Leu Gly Ile Ala Val Glu Thr Phe Val Met Arq Gly Ala 100 105 taaagtttct gatttaacgg Ala Asp Arg Ile Ala Arg Leu Val1 Pro His Met Ile Arq Ala 110 Phe Gin Le u Gly Ala Met Pro WO 01/00805 WO 0100805PCTIBOOIOO926 Ala Trp Arq 145 Ala His Ala Le u Al a 225 Ala Le u Leu 115 Ala Ala Ala Leu Ala 195 Thr Ala Ile Asp Le u Met Arg Arg Pro 180 Le u Thr Ser Ala Ala 260 Ile Al a Gly 150 Ser Ile Ile Pro Gly 230 Thr Asp Ile Ile 135 Thr Lys Thr Le u Asp 215 Vali Val Pro Se r 120 Gi y Le u Val1 Se r Ala 200 Pro Th r Le u Lys Val Giy Thr 155 Ala Ile Ala Gly Le u 235 As n Val1 Giy 125 Pro Gin Ile Gin Ser 205 Met Val Phe Arg Th r Al a Ile 160 Arg Val1 Gi y Thr Gly 240 Gly <210> 647 <211~> 715 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(715) <223> RXN02991 <400> 647 ttgtcgtgtt cqcqatcttc gccgttqgct tcatgattct qcttgggcaa actctctqag cgtttgqcqg tgaagaagta cqc gca aca qtc ctc tac gat gct Arq Ala Thr Val Leu Tyr Asp Ala aac ctc ata atc acc att ctc acg Asn Leu Ile Ile Thr Ile Leu Thr ttc tqq att qqc tcc atq ctt tca Phe Trp Ile Gly Ser Met Leu Ser 45 cct gga cca Pro Gly Pro 15 gtg gtt ctg Val Val Leu 30 gqc aac ggc Gly Asn Gly gaccctgcct atgggccttq atg gct aat aca 9tt Met Ala Asn Thr Val 1 aag ggc cgt cga ttc Lys Gly Arg Arg Phe gga ttg gcq ctc ctc Gly Leu Ala Leu Leu caa ctc gat gcc aac Gin Leu Asp Ala Asn 115 163 211 259 WO 01/00805 PCT/IBOO/00926 aaa tgg act ccg ttc atc aat tcc caa acc tgg acc acc tac att ctt 307 Lys Trp Thr pro Phe Ile Asn Ser Gin Thr Trp Thr Thr'Tyr Ile Leu 60 cct ggt ttg tgq ggc acg Ctg aaa tct gcc qtg ttc teg gtg atc ttq 355 Pro Gly Leu Trp Gly Thr Leu Lys Ser Ala Val Phe Ser Val Ile Leu 75 80 qet ctg gtc atg ggt aec gca etg ggt ett ggc cgt atc tct gaa atc 403 Ala Leu Val Met Gly Thr Ala Leu Gly Leu Gly Arg Ile Ser Giu Ile 95 100 agg att etc cgc tgg ttc tgc gcc gtc atc atc qag act ttc cga gcc 451 Arg Ile Leu Arg Trp Phe Cys Ala Val. Ile Ile Giu Thr Phe Arg Ala 105 110 115 att ccg gtt ctg atc ctc atg att ttc gcc tac eag atg ttc gcc cag 499 Ile Pro Val Leu Ile Leu Met Ile Phe Ala Tyr Gin Met Phe Ala Gin 120 125 130 tac aac ate gtg ccg teg agc cag etc gcg ttc gcc gcc gtq gta ttc 547 Tyr Asn Ile Val Pro Ser Ser Gin Leu Ala Phe Ala Ala Val Val Phe 135 140 145 gqt ctg ace atg tac aac ggt tct gtq ate gca gag att ctg egt tet 595 Gly Leu Thr Met Tyr Asn Gly Ser Val Ilie Ala Giu Ile Leu Arg Ser 150 155 160 165 ggt ate gct tee etg cet aag ggg cag aag gaa gea geg att geg ttg 643 Gly Ilie Ala Ser Leu Pro Lys Gly Gin Lys Giu Ala Aia Ile Ala Leu 170 175 180 ggt atg tet tet agq caa ace ace tgq tea ate ttg ttg cet eag gee 691 Gly Met Ser Ser Arq Gin Thr Thr Trp Ser Ile Leu Leu Pro Gin Ala 185 190 195 gtg get gea atg etc eca qeg ttg 715 Val Aia Ala Met Leu Pro Ala Leu 200 205 <210> 648 <211> 205 <212> PRT <213> Corynebacterium giutamicum <400> 648 Met Ala Asn Thr Val Arg Ala Thr Val Leu Tyr Asp Ala Pro Gly Pro 1 5 10 Lys Gly Arg Arg Phe Asn Leu Ilie Ilie Thr Ilie Leu Thr Val Val Leu 25 Gly Leu Ala Leu Leu Phe Trp Ilie Gly Ser Met Leu Ser Gly Asn Gly 40 Gin Leu Asp Ala Asn Lys Trp Thr Pro Phe Ilie Asn Ser Gin Thr Trp 55 Thr Thr Tyr Ile Leu Pro Gly Leu Trp Gly Thr Leu Lys Ser Ala Vai 70 75 WO 01/00805 WO 0100805PCTJIBOO/00926 Phe Arg Glu Gin Ala 145 Glu Ala L eu Leu Ile Al a Gin Phe Ser 165 Le u Ala Leu Ilie Pro As n 135 Le u Ile Met Al a Val1 Le u Val 120 Ile Thr Ala Se r Ala 200

M

A

1 <210> 649 <211> 245 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> <223> RXN02992 <400> 649 atc qtc cct ttg qgc aac aca ctg Ilie Val Pro Leu Gly Asn Thr Leu 1 5 atc gcc tct qtc att gga gtt ggc Ile Ala Ser Vai Ile Gly Val Gly acc atc gaa aat cac gcc aac atg Thr Ilie Glu Asn His Ala Asn Met 40 9cc gtt 990 ttc atg att ctg acc Ala Val Gly Phe Met Ile Leu Thr 55 aaa ctc tct gag cgt ttg gcg gtg Lys Leu Ser Glu Arg Leu Ala Val gca <210> 650 let ~rg .05 e u al1 let er ~er ~et atc Ilie ;aa Glu 25 cta Leu ctg Le u aag Lys Gly 90 T rp Ile Pro Tyr Leu 170 Arg Leu gca Ala 10 qcc Al a ttt Phe cct Pro a ag Ly s Gly Val1 110 Phe Le u Val1 Gin Trp 190 Leu Ile Ala Ala Ile Lys 175 Ser Gly Ile Tyr Phe Ala 160 Giu Ile ctg act aag aac acc acc Leu Thr Lys Asn Thr Thr.

tcc ctg ctg atg aaa 9cc Ser Leu Leu Met Lys Ala gtc gtg ttc gcg atc ttc Val Val Phe Ala Ile Phe atg ggc ctt ggc ttg ggc Met Gly Leu Gly Leu Gly taatqgctaa tacagttcqc WO 01/00805 <211> 74 <212> PRT <213> Corynebacterium glutamicun <400> 650 Ile Val Pro Leu Gly Asn Thr Leu Ile Ala Leu 1 5 10 Ile Ala Ser Val Ilie Gly Val Gly Glu Ala Ser 25 Thr Ile Glu Asn His Ala Asn Met Leu Phe Val 40 Ala Val Gly Phe Met Ile Leu Thr Leu Pro Met 55 Lys Leu Ser Glu Arg Leu Ala Val Lys Lys <210> 651 <211> 792 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(769) <223> RXN02996 <400> 651 tcctgccaca gaatctgcct gctgctccat taactaaaaa tcgattagga gtcgatgaaa aagtcggcqc caacgaggag PCTIBOO/00926 Thr Lys Asn Thr Thr Leu Leu Met Lys Ala Val Phe Ala Ile Phe Gly Leu Gly Leu Gly ag t Ser gcc Ala gga Gly gtg Val1 Gly gta Val aa g Ly s act Thr ctt Le u t gg Trp ctt Leu ctt Leu cta Leu ctc Le u ttc Phe ca g Gin gg t Gi y tct Ser ggt Gly ctt Leu atc Ile ctg Le u tac Tyr qq c Gly aaa Lys gg t Gly gca Al a a cg Th r qg t Gi y act Thr gag Gi u ga t Asp 30 ctc Le u ctq Le u ttc Phe qtc Val1 ct t Le u 15 aag Lys gtc ValI t cg S er tac T yr qtg ValI 95 gcg Ala aag Ly s ct C Leu aat Asn tgq Trp 80 ttt Phe ttgcaggcta gaatagaggg atg aac aac aac gtg Met Asn Asn Asn Val 1 gca cta gag aaa caa Ala Leu Giu Lys Gin tgg tat ctc atc gcg Trp Tyr Leu Ile Ala cca. cat atc cgt gga Pro His Ile Arg Gly gtc gcg gag gat gcc Val Ala Giu Asp Ala ttg ggc acc atc ggt Leu Gly Thr le Gly aag cgc acg tgg atq Lys Arg Thr Trp Met 100 115 163 211 259 307 355 403 WO 01/00805 WO 0100805PCTIBOOIOO926 t gg Trp qcc Ala aac Asn 135 gg t Gi y gct Ala cag Gin ttq Le u att Ile atc Ile 120 att Ile ct a Le u gaa Glu cgc Arg ct t Leu 200 tca Se r 105 tg Trp qqc Gly tct Ser atg Met 9 ca Al a 185 gtc Val1 tqg Trp atg Met atg Met t cq Ser cgc Arg 170 ctt Le u ga c Asp tgc Cys 110 acc Th r gtg Val1 gCC Ala cca Pro cag Gin 190 g cc Ala gtc Val a cc Thr att Ile cgc Arg gac Asp 175 caa Gin cgc Arg act ctt Thr Leu agc acc Ser Thr gca gcg Ala Ala 145 agt gat Ser Asp 160 ctt gat Leu Asp agc aac Ser Asn atc gcc Ile Ala 451 499 547 595 643 691 739 Arg Arg Arg 210 cgt gaa cag gat gca caa gq gag cag gct Arg Glu Gin Asp Ala Gin Giy Glu Gin Ala 215 220 t ga <210> 652 <211> 223 <212> PRT <213> Corynebacterium qiutamicum (400> 652 Met Asn Asn Asn Val Ser Asp Gin Lys Leu 1 5 Ala Leu Glu Lys Gin Ala Ala Lys Thr Leu 25 Trp Tyr Leu Ile Ala Gly Val Val Leu Phe 40 Pro His Ile Arq Gly Val Met Gly Trp Gin 55 Val Ala Glu Asp Ala Gly Ile Thr Leu Gly Leu Gly Thr Ile Gly Vai Phe Leu Leu Ser Lys Arq Thr Trp Met Ala Trp Ile Ser Trp taagttctag ttcaagcggt Gly Leu Ile Le u Tyr Gi y Phe Lys Gi y Al a Thr Gly Thr Ser Glu Asp Leu Leu Phe Val Cys WO 01/00805 WO 0100805PCTIBOOIOO926 100 105 Leu Val Phe Ala Val Phe Ala Ile Trp Met Arg 115 120 Thr Gin Val Asn Phe Vai Asn Ile Gly Met Met 130 135 Ala Ile Leu Ala Val Trp Gly Leu Ser Ser Val 145 150 155 Asp Arg Gin Met Giu Ile Ala Giu Met Arq Ala 165 170 Asp Gly Val Ala Ala Thr Gin Arg Ala Leu Leu 180 185 Asn Pro Glu Asp Asn Pro Leu Leu Val Asp Asp 195 200 Ala Arg Arg Arg Glu Arq Giu Gin Asp Ala Gin 210 215 <210> 653 <211> 1017 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (994) <223> RXN03126 <400> 653 tcttggacaa cattacccca cacacgggac actcaaacgt qatacccaaa aatcactcca ccaccactag ggtgtcttat Gin Le u 140 Ile Giu Giu Arg Giy 220 110 Thr Thr Val Ile Ala Arg Pro Asp 175 Gin Gin 190 Ala Arg Gin Ala ca g Gin gac Asp gat Asp tgg Trp tgg Trp gat Asp ca g Gin aa g Ly s CgC Arg gt Gly gcatgaattc acctqtcgtc qtq cag gaa caa tcq Vai Gin Giu Gin Ser 1 atc act gcc gtq aaa Ile Thr Ala Val Lys ttt ggc gct gcc tgg Phe Gly Ala Ala Trp ttg tqq ctg caa ctc Leu Trp Leu Gin Leu tcc gtc ctt ggg ccc Ser Vai Leu Gly Pro ctc gcc ctc ggc ttg Leu Ala Leu Gly Leu ctq Leu qt c Val1 gaa Giu ccc Pro ctc Le u 150 ttc Phe ttc Phe atc Ile caa Gin caa Gin 230 aac Asn gcc Ala gtc Val WO 01/00805 tac tcc gtg Tyr Ser Val acc gtc ggc Thr Vai Gly 105 ggc tcc qac Gly Ser Asp 120 tcg qcg ctg Ser Ala Leu 135 ttc ctc gcc Phe Leu Ala ccc cqa ccc Pro Arg Pro ctc ctg gtg Leu Leu Vai 185 gcc aco cgc Ala Thr Arg 200 ctc ctc ttc Leu Leu Phe 215 tcc cag agc Ser Gin Ser ccg ctc tac Pro Leu Tyr gac ctc ccc Asp Leu Pro 265 ggc ctt ggc Giy Leu Gly 280 ct g Le u ctq Le u atc Ilie tcc Ser cac His ctc Leu 170 atc Ile tac Tyr tac Tyr gca Al a cac His 250 gcc Ala ct t IA u t tc Phe atc Ile ttc Ph e gtc Val aac Asn 155 ggc Gly aac Asn cgc Arg qt c Val1 gaa Giu 235 tac T yr tac Tyr gct aaa Ly s att Ile ata Ile cac His 140 ctg Le u tgg Trp qg C Gly ga c Asp ac c Th r 220 atc Ile ctc Le u cac His ctc PCTIBOO/00926 atc Ile tgg Trp gat Asp 125 gtg Val1 gtc Val gac Asp gtg ValI gtc Val1 205 ccc Pro gg c Giy ga a Glu tgq T rp ctc ccc Pro aac As n 110 aac Asn tac Tyr atc Ile qt c Val1 t gg Trp 190 tcc Se r at c Ile aac Asn att Ile t gg Trp 270 gcq Ala atc Ile 95 ttc Ph e gag Glu aga Arg t99 Trp ctc Le u 175 gta Val ccg Pro qt c Val1 cqc Arq gt c Vai 255 atc Ile atg Met g cg Aia atc Ile gga Gly ct C Leu gtc ValI 160 ctg Leu gtc ValI t tg Le u t gg T rp gca Ala 240 cg C Arg gt c Val1 aag gaa Glu tcc Ser ct C Le u Val1 145 att Ile atc Ile atg Met ct a Le u atq Met 225 cgc Arg gcc Al a ctc Leu caa ttt Phe qga Giy atc Ile 130 t qg Trp ct C Le u atc Ile ttC Phe gaa Giu 210 act Thr ct C Leu ccc Pro gcc Ala t gq ctg Leu tgc Cys 115 aaa Lys aaa Lys atg Met ccc Pro ttc Phe 195 gcc Ala tcc Ser gcc Aia atg Met ttc Ph e 275 cga

CCC

Pro 100 atc Ile cag Gin caa Gin at g Met gca Ala 180 ggc Giy gga Gly acc Thr gaa Giu gtc Val1 260 aca Thr ttc cac His aaa L ys CtC Le u g cc Ala atc Ile 165 a tg Met atC Ile a cc Th r ctg Leu ctC Leu 245 ggc Gly ttc Phe cq c 403 451 499 547 595 643 691 739 787 835 883 931 979 1017 Ala Leu Leu Lys Gin Trp Arq Phe Arg 290 gtc agc tac tgg gta taagqagcac cacatggtat cca Val Ser Tyr Trp Vai 295 <210> 654 <211> 298 <212> PRT <213> Corynebacterium giutamicum WO 01/00805 <400> 654 Val Gin Giu Gin 1 Ile Thr Ala Val Phe Gly Ala Ala Leu Trp Leu Gin Ser Val Leu Gly Leu Ala Leu Gly Giu Phe Leu Pro 100 Ser Gly Cys Ile 115 Leu Ile Lys Gin 130 Vai Trp Lys Gin 145 Ile Leu Met Met Ile Ile Pro Ala 180 Met Phe Phe Gly 195 Leu Giu Ala Giy 210 Met Thr Ser Thr 225 Arg Leu Aia Giu Ala Pro Met Val 260 Leu Ala Phe Thr 275 Gin Trp Arg Phe 290 <210> 655 PCT[IBOO/00926 Ser Lys Gin Lys*Asp Leu Gin Ala Asp Ile Ala Arg 10 Lys Trp Le u Pro Le u His Lys Leu Ala Ile 165 Met Ile Thr Leu Leu 245 Gly P he Arg Asp Asp Trp 55 Trp T1'yr Thr Giy Se r 135 Phe Pro Le u Ala Leu 215 Se r Pro Asp Gly S er 295 Th r Ile 40 Gin Ile Ser Val Ser 120 Ala Le u Arg Leu Thr 200 Leu Gin Leu Leu Leui 280 Tyr Val1 25 Val1 Asp Thr Val1 Gi y 105 Asp Le u Al a Pro ValI 185 Arq Phe Se r Ty r Pro 265 Gly Trp Pro Arg Ile Ile Le u 90 Leu Ile Se r His Leu 170 Ile Tyr Tyr Ala His 250 Ala Le u Val Asn Giy Ly s Ala 75 Phe Ile Phe ValI Asn 155 Gi y As n Arg Val1 Giu 235 Tyr Tyr Ala Ser Phe Gin Thr Ly s Ile Ile His 140 Le u Trp Gly Asp Th r 220 Ile Le u His Le u Gin Ly s Arq Gly Ile Trp Asp 125 Val1 Val1 Asp Val1 Val 205 Pro Gi y Giu Trp Leu 285 Ser Gin Tyr Vai Pro Asn 110 Asn Tyr Ile Val1 T rp 190 Ser Ile Asn Ile Trp 270 Ala Met His Arg Met Ile Phe Giu Arg Trp Le u 175 Val1 Pro Val1 Arg Val1 255 Ile Met Thr Giu Arg Ala Ala Ile Gly Leu Val1 160 Leu Val Leu Trp Ala 240 Arg Val1 Lys WO 01/00805 <211> 927 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (101)..(904) <223> RXN00443 <400> 655 taaacaagga gcgjtttaatt tccacccaai cccactgaaa gctgattaaq tactqaactt accgtaggaa aggcacicag PCT/iBOO/00926 gittcccacc ccacagttat cg i Arg gta Va 1 tct Ser qcg Al a ga a Glu gcg Al a tct Se r gcc Al a acc T hr gac Asp 150 acc Thr gag cga Arg g cg Ala gci Ala g cg Ala gac Asp c tg Leu g cg Al a qg C Gly g ca Ala 135 icc Ser cac His gaa gci Ala tgc Cys acc Thr tct Ser aat Asn gt Val1 gac Asp g ca Al a 120 gac Asp ctc Leu gag Glu gcc cic Le u tci Ser ga a Glu ttg Le u cct Pro cg c Arg atc Ile 105 acc Th r ggc Gly qti Val gct Al a aat tac Tyr tct Se r gca Ala acc Th r gat Asp cag Gln g cc Ala t cg Se r aac Asn gct Ala tig Leu 170 gt agt Ser t ca Ser qct Ala aac As n gca Al a 75 atc Ile aac Asn aag Lys ccc Pro atc Ile 155 gac Asp gct tic Phe gac Asp ggg Gly gt Val1 60 a ag Lys agc Ser aig Meti gi c Val1 Gly 140 ig c Cys tac Tyr gat aic I le acc Thr gt Val1 45 gct Ala t ig Leu gaa Glu gac Asp aic Ile 125 gaa Gl u gcc Al a gca Ala gic act ati Thr Ile 15 gca agc Ala Ser 30 tcc gga Ser Gly gag gai Glu Asp gag iii Glu Phe ggc gca Gly Ala 95 gat gcg Asp Ala 110 gci acc Ala Thr att ica Ile Ser cct gaa Pro Glu gac aic Asp Ile 175* gcc acg icg Ser act Thr acc Th r ci c Leu aac Asn 80 cca Pro ctg Leu aac As n gag Glu gitt Val 160 gag Glu gig Val1 1 gcq Ala acc Th r gci Al a cg Ala iic Phe ict Ser gca Ala aag Lys cti Leu 145 cca Pro i tg Le u ggi Gly acc Thr agc Ser gci Al a gci Ala gati Asp cti Le u cig Le u 130 gcc Ala tgt Cys aac Asn aic I le cag Gln qii Val1 gcg Ala gg t Gly cig Le u cca Pro *115 gig Val1 gac Asp gga Gly acc Th r aac aaa agt atc Asn Lys Ser Ile ica ctc Ser Leu aai gcc Asn Ala itc gci Phe Ala iic aac Phe Asn ict tcc Ser Ser itc aii Phe Ile 100 gaa t Glu Phe cig gic Leu Val gic aag Val Lys acc aic Thr Ile 165 agc tci Ser Ser 180 115 163 211 259 307 355 403 451 499 547 595 643 691 aag ati icc acc ggi gca WO 01/00805 WO 0100805PCTIBOOIOO926 Glu Glu Ala Asn Val Ala Asp Val Ala Thr Lys Ile Ser Thr Gly Ala 185 190 195 gtt gat gcg ggc ttt gtt tac caa acc gac gct cag tct ttg gct aea 739 Val Asp Ala Gly Phe Val Tyr Gin Thr Asp Ala Gin Ser Leu Ala Lys 200 205 210 act ceq gac aac act gtc ett gee ctc gaa ggt atc gac gcc eec gea 787 Thr Gin Asp Asn Thr Val Ile Glu Leu Glu Giy Ile Asp Ala Asn Giu 215 220 225 tac cca etq gca ttq acc acc ecc gqt gaa gac eec gag gte gcg aag 835 Tyr Pro Met Ala Leu Thr Thr Thr Gly Giu Asp Asn Glu Val Ala Lys 230 235 240 245 gct ttc gca gag ttc ctc egc agc gat cgt qcc eeq gag atc ctt gcc 883 Ala Phe Ala Giu Phe Leu Ser Ser Asp Arg Ala Lys Giu Ile Leu Ala 250 255 260 egc tat ggt ttt ggc ace eac taaaaaggct cgtcgaagcg aga 927 Ser Tyr Gly Phe Gly Thr Asn 265 <210> 656 <211> 268 <212> PRT <213> Corynebacterium giutarnicum <400> 656 Val Asn Lys Ser Ile Arg Arg Ala Leu Tyr Ser Phe Ile Thr Ilie Ser 1 5 10 Ala Gly Ilie Ser Leu Val Ala Cys Ser Ser Ser Asp Thr Ala Ser Thr 25 Thr Thr Gin Asn Ala Ser Ala Thr Giu Ala Ale Gly Val Ser Gly Thr 40 Ala Ser Val Phe Ala Ala Ala Ser Leu Thr Asn Val Ale Giu Asp Leu 55 Ala Ala Ala Phe Asn Glu Asp Asn Pro Asp Ala Lys Leu Glu Phe Asn 70 75 Phe Ala Gly Ser Ser Ala Leu Val Arg Gin Ilie Ser Giu Gly Ala Pro 90 Ser Asp Leu Phe le. Ser Ala Asp Ile Ala Asn Met Asp Asp Ala Leu 100 105 110 Ala Leu Pro Giu Phe Ala Gly Ala Thr Ser Lys Val Ile Ala Thr Asn 115 120 125 Lys Leu Val Leu Val Thr Ala Asp Gly Asn Pro Giy Giu Ile Ser Glu 130 135 140 Leu Ale Asp Val Lys Asp Ser Leu Val Ala Ile Cys Ala Pro Glu Val 145 150 155 160 Pro Cys Giy Thr Ile Thr His Giu Ala Leu Asp Tyr Ala Asp Ile Giu WO 01/00805 WO 0100805PCTIBOO/00926 Le u Ile Gin Ile 225 Asn Lys As n Ser Ser 210 Asp Glu Giu Ser 180 Gly Al a Asn Al a Leu 260 165 Ser Aila Lys Giu L ys 245 Ala Giu Asp Gin 215 Pro Phe Tyr Ala Ala 200 Asp Met Ala Gly As n 185 Giy Asn Ala Glu Phe 265 170 Val Ala Phe Val Thr Val Leu Thr 235 Phe Leu 250 Gly Thr Asp Val Ala 190 Tyr Gin Thr 205 Ile Giu Leu 220 Thr Thr Gly Ser Ser Asp Asn 175 Thr Asp Giu Giu Arq 255 <210> 657 <211> 960 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (937) <223> RXN00444 <400> 657 tacccaatgg cattgaccac caccggtgaa qacaacgagg ttcctcagca gcgatcgtgc caagqaqatc cttgccagct cgt Arq acc Th r cag Gin tta Leu cta Leu gag Glu cg a Arg ctg Le u atc Ile gga Gly tgc Cys cg t Ar g gag Giu atg Met t gg Trp gca Ala att Ile cac His aat Asn ctg Le u gat Asp cgg Arg gtg Val1 ccc Pro cat His 15 ctq Leu cgg Arg tta Leu ggt Gly agg Arg 95 atc Ile atc Ile tct Se r tct Ser ttc Phe 80 gtg ValI tagcgaaggc tttcgcagaq atg gtt ttg gca caa Met Val Leu Ala Gin 1 ctc cca ggg tgg ttg Leu Pro Gly Trp Leu att gga cct att ttt Ile Gly Pro Ilie Phe tgg gag ttg ctt acc Trp Giu Leu Leu Thr atc gga act gct ctg Ile Gly Thr Ala Leu ccg cta gcg ttg gcg Pro Leu Ala Leu Ala aca tca gtt ttg acg Thr Ser Val Leu Thr 100 115 163 211 259 307 355 403 gtg Val gcg Al a gat Asp gcc Al a 150 gca Al a ggC Gly atg Met ggC Gly acc Thr 230 atg Met ctc Le u WO 01/00805 ctg gtt tat Leu Val Tyr 105 ctg act ttt Leu Thr Phe 120 cag gtt gga Gin Vai Gly 135 cag gtg ttt Gin Val Phe ctg cgt ggc Leu Arg Gly gca acc cgc Ala Thr Arg 185 cct gga att Pro Gly Ile 200 gag tat ggt Giu Tyr Gly 215 cgc acc att Arg Thr Ile gat aaa gcc Asp Lys Ala atc att gga Ile Ile Gly gcq cct ttg Ala Pro Leu ctg tqg ggc Leu Trp Gly ttq ccg att Leu Pro Ile 140 gta gcg ttg Val Ala Leu 155 att cca aaa Ile Pro Lys 170 tgg gag atc Trp Giu Ilie ttc acc ggt Phe Thr Gly gcg aca ctg Ala Thr Leu 220 ccg ttg cat Pro Leu His 235 ttg gga gcg Leu Gly Ala 250 gcc atc gga Ala Ilie Gly gtg Val1 agg Arg 125 gca Ala c ca Pro ca g Gin atg Met atg Met 205 act Thr att Ile gtg ValI gcg Ala ttg tcg Leu Ser 110 cgt ggt Arg Gly ttt acc Phe Thr ttt ttc Phe Phe ttt gag Phe Giu 175 cac aag His Lys 190 att ttg Ile Leu ttt gct Phe Ala gag ctt Giu Leu att atg Ile Met 255 tta cga Leu Arg 270 tct Ser 115 t ct Ser gtg Val gtg Val1 q ct Ala c cg Pro 195 agg Arg g ca Ala t cc Ser gqt c ValI aag Lys 275 gg t Gi y tgg Trp qtq Val act Th r act Th r 180 ctg Le u gcc Al a ggt Gly aat Asn tat Tyr 260 St g Val PCTIIBOO/00926 ttg 451 Leu ctt 499 Leu ttt 547 Phe act 595 Thr 165 qgaa 643 Giu gcg 691 Ala ttg 739 Leu g tt 787 Val1 gac 835 Asp 245 gtc 883 Val aga 931 Arq 265 aag gtt Lys Val taattgatgt ctcgttcgcc gga <210> 658 <211> 279 <212> PRT <213> Corynebacteriun giutarnicum <400> 658 Met Val Leu Ala Gin Thr Lys Lys Ala Arg Arg Ser Glu Asn His Ile 1 5 10 Leu Pro Gly Trp Leu Leu Ilie Pro Ala Thr Leu Ala Met Leu Leu Ile 25 WO 01/00805 WO 0100805PCTIBOOIOO926 Ile Gly Pro Ilie Phe Ala Leu Leu Leu Gin Ile Pro Trp Asp Arq Ser 40 Trp Glu Leu Leu Thr Ala Pro Glu Ser Leu Gly Thr Ala Arg Leu Ser 55 Ile Gly Thr Ala Leu Phe Ser Thr Ala Leu Cys Ala Ile Val Gly Phe 70 75 Pro Leu Ala Leu Ala Leu His Leu Tyr Giu Arq Ser His Pro Arg Val 90 Thr Ser Val Leu Thr Val Leu Val Tyr Ala Pro Leu Val Leu Ser Pro 100 105 110 Val Val Ser Gly Leu Ala Leu Thr Phe Leu Trp Gly Arg Arg Gly Phe 115 120 125 Leu Gly Ser Trp Leu Asp Gin Val Gly Leu Pro Ile Ala Phe Thr Thr 130 135 140 Thr Ala Val Val Phe Ala Gin Vai Phe Val Ala Leu Pro Phe Phe Ile 145 150 155 160 Ser Thr Val Thr Thr Aia Leu Arq Gly Ile Pro Lys Gin Phe Glu Giu 165 170 175 Ile Ala Ala Thr Giu Gly Ala Thr Arq Trp Giu Ilie Met His Lys Met 180 185 190 Ile Ilie Pro Leu Ala Met Pro Gly Ile Phe Thr Gly Met Ile Leu Gly 195 200 205 Phe Ala Arg Ala Leu Gly Glu Tyr Gly Ala Thr Leu Thr Phe Ala Gly 210 215 220 Asn Ilie Ala Gly Val Thr Arg Thr Ile Pro Leu His Ile Glu Leu Gly 225 230 235 240 Leu Ser Ser Asn Asp Met Asp Lys Ala Leu Gly Ala Vai Ile Met Leu 245 250 255 Leu Ala Val Tyr Val Leu Ile Ilie Gly Ala Ilie Gly Ala Leu Arg Leu 260 265 270 Phe Ser Lys Val Arq Lys Val 275 <210> 659 <211> 594 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (594) <223> RXN00193 <400> 659 aaa Lys 1 gtc Val ct C Leu cgc Arg tat Tyr act Thr ctt Leu ctg Leu gtc Val1 cca Pro 145 ctc Le u act Thr aat Asn WO 01/00805 gct ttc tnc caa cgc Ala Phe Xaa Gin Arg 5 ctc gtg gtc atc gtc Leu Val Val Ile Val ttq gcg tgg ttg ctg Leu Ala Trp Leu Leu aca gtc ttc ttt atg Thr Val Phe Phe Met acc tgg cag acc atg Thr Trp Gin Thr Met 70 att agc gcg gac tgg Ile Ser Ala Asp Trp aac tgg cag ctc atc Asn Trp Gin Leu Ile 100 caa aac gtc cca cca Gin Asn Val Pro Pro 115 dac aag tgg gag atg Asn Lys Trp Giu Met 130 tcc atc acc atc tgc Ser Ilie Thr Ile Cys 150 ttc gac cag aac ctg Phe Asp Gin Asn Leu 165 gag atg gtg gcg ctc Giu Met Val Ala Leu 180 gtc gag ggc gtc ggt Val Giu Giy Val Giy 195 gaa ggt Glu Gly tcc gtg Ser Val acc cgc Thr Arg 40 ccg aac Pro Asn 55 atc aac Ile Asn aaa ttc Lys Phe ggc tac Gly Tyr gag ctc Glu Leu 120 ctg cgg Leu Arg 135 ctc ttt Leu Phe gcg .ctg Ala Leu ttc Phe atc Ile aaa Lys ctt Le u gcc Aila Giy atg Met 105 att Ile cac His ttg Leu acc Thr atc Ile 10 aca Thr ctc Le u atc Ile gtg Val1 tac Tyr 90 atg Met gag Glu gtc Val1 act Thr aac Asn 170 t ca Ser gtc Vali cgc Arg ggc Gi y ctt Le u 75 gcc Ala atc Ile gct Ala act Thr ttg Le u 155 ggc Giy gcc Ala aac Asn ggt Gly ggC Gly t cg Ser ggc Gly att Ile gcc Ala att Ile 140 t cg Se r gct Al a ttC Phe atc Ile acc Th r att Ile cac His ctc Leu tac Tyr gaa Giu 125 c cg Pro aac Asn c ct Pro ttc Phe 9CC Ala ttt Phe ctg Le u gcc Ala atg Met gjcc Ala gac Asp gtc Val ttt Phe 9gg Gly 175 P4 acc Th r ttC Phe ttc Phe 99 t Gly acg Th r ct a Leu ggc Gly gg C Gly a tg Met aag Lys 160 caa Gin CTIiBOOIOO926 48 96 144 192 240 288 336 384 432 480 528 576 aac atc atc aac acg ctg ttt aac cgt atg Asn Ile Ile Asn Thr Leu Phe Asn Arg Met <210> 660 <211> 198 'z212> PRT <213> Corynebacterium glutamicum <400> 660 Lys Ala Phe Xaa Gin Arg Glu Gly Phe Ile Ser Ala Phe Gly Phe Thr 1 5 10 WO 01/00805 WO 0100805PCT/IBOO/00926 Val Leu Val Val Ilie Val Ser Val Ile Thr Vai Asn Ile Phe Ala Phe 25 Leu Leu Ala Trp Leu Leu Thr Arg Lys Leu Arg Gly Thr Asn Phe Phe 40 Arg Thr Vai Phe Phe Met Pro Asn Leu Ile Gly Giy Ile Val Leu Gly 55 Tyr Thr Trp Gin Thr Met Ile Asn Ala Val Leu Ser His Tyr Ala Thr 70 75 Thr Ile Ser Ala Asp Trp Lys Phe Giy Tyr Ala Gly Leu Ile Met Leu 90 Leu Asn Trp Gin Leu Ilie Giy Tyr Met Met Ile Ile Tyr Ile Ala Giy 100 105 110 Leu Gin Asn Val Pro Pro Giu Leu Ile Giu Ala Ala Giu Leu Asp Gly 115 120 125 Vai Asn Lys Trp Giu Met Leu Arg His Vai Thr Ile Pro Met Val Met 130 135 140 Pro Ser Ilie Thr Ile Cys Leu Phe Leu Thr Leu Ser Asn Ser Phe Lys 145 150 155 160 Leu Phe Asp Gin Asn Leu Ala Leu Thr Asn Gly Ala Pro Giy Gly Gin 165 170 175 Thr Giu Met Val Ala Leu Asn Ilie Ilie Asn Thr Leu Phe Asn Arg Met 180 185 190 Asn Val Giu Gly Val Gly 195 <210> 661 <211> 1053 <212> DNA <213> Corynebacterium qiutamicum <221> CDS <222> (101)..(1030) <223> RXN01298 <400> 661 cttaaacgtc accttattta tgcattatgt tggtttcaga ctcgaacaat tcaattagaa aacactaatc ggacatttag gtcacataac atttccgctc gtg tcc aca tta att 115 Val Ser Thr Leu Ile 1 tct gaa ccc gag gtg gat aag cta cgt aaa cgt gcc aag aga tca agq 163 Ser Giu Pro Giu Val Asp Lys Leu Arg Lys Arg Ala Lys Arg Ser Arg 15 cgg aca gaa tgg tgg ctt gcc gcc qca ctt ctt gcc cca aac ttg ctt 211 Arg Thr Giu Trp Trp Leu Ala Ala Ala Leu Leu Ala Pro Asn Leu Leu ctc Leu tcc Se r ttt Phe gt t Val gtg Val1 cgt Arg gct Ala ttg Leu 150 tac Tyr t 99 T rp ggg Gly g cg Ala a cg Thr 230 gac Asp acc Thr WO 01/00805 ttg 9cc atc Leu Ala Ilie ttt ttc aac Phe Phe Asn gat aac tac Asp Asn Tyr tta aac acc Leu Asn Thr ctc ggt ttg Leu Gly Leu 105 aac ttt gtg Asn Phe Val 120 gcc att qqt Ala Ile Gly 135 qtt cag gac Val Gin Asp caa aac cct Gin Asn Pro aag aac ttg Lys Asn Leu 185 cta aac aag Leu Asn Lys 200 tgg aca cgt Trp Thr Arg 215 ttc ttc ctt Phe Phe Leu atc att cac Ile Ile His acc ttg gtt Thr Leu Val 265 ttt Phe tgg Trp gtt ValI gtc Val1 Ctc Le u cg t Arg gtt ValI ttg Leu aac Asn 170 ggC Gly gat Asp ttt Phe t ct Ser acc Thr 250 tac Tyr a cg Thr aac Asn gag Giu 75 atc Ile ctg Leu tcc Ser qct Al a ctg Le u 155 tgg Trp tac Tyr ttg Le u tgg Trp att Ile 235 atg Met cag Gin PCTIBOO/00926 tat Tyr att Ile 60 ttc Phe ttc Ph e 9CC Ala atg Met ttc Phe 140 gqa Gly qca Ala tcc Se r tct Ser a ag Lys 220 act Thr act Th r gtg ValI C99 Arg 45 tcc Ser ttc Phe a cg Thr atg Met gtg Val 125 cag Gln cgc Arg ttg Leu ttt Phe gag Giu 205 gtt Val qt c ValI cg t Arg tac Tyr 30 cca Pro tcg Ser act Th r gca Ala ttg Le u 110 ttt Phe ttc Phe at c Ile ttc Phe gtt Val 190 9CC Ala act Thr acg Thr 99 t Gly acc Thr 270 C tg Leu ccc Pro cqt Arg tgt Cys 95 ttg Leu gCC Ala gt t Vai ggC Gly atg Met 175 atc Ile gca Ala Ct t Le u ctg Le u ggc Giy 255 gag Glu tta Le u aca Th r agt Se r 80 gct Al a gat Asp Ccg Pro ttt Phe gtt Vai 160 gt 9 Val1 tac Tyr ccg Pro Ccg Pro aac Asn 240

CCC

Pro act Thr gat Asp tca Se r g ac Asp gtg Val1 cag Gin ttt Phe gac Asp 145 ga t Asp a cg Thr ct 9 Le u gtg Val cag Gin 225 t Cg Ser ttg Le u t tc Phe aac Asn acC Th r act Thr atc Ile a ag Lys gtg Val 130 cct Pro t Cg Ser ttc Phe gct Ala ga t Asp 210 ctt Le u gtt Vai ggt Gly acc Thr ttc Phe ttc Phe ctc Le u 99 a Gly ctt Leu 115 att Ile aa t Asn cca Pro act Th r gca Ala 195 ggC Gly cgc Arg cag Gin aac As n aac Asn 275 Cgg Arg att Ile caa Gin tcg Se r 100 ttc Phe t CC Ser ttt Phe cag Gin ttc Phe 180 ttg Le u gcg Ala C ca Pro gtc Val1 99 t Gly 260 tat Tyr ttg Le u 999 Gl y gtt ValI a tg Met g9C Gl y qgt Gly 99 t Gly ttc Phe 165 gtq Val1 ca 9 Gin agc Se r acc Th r ttc Ph e 245 acq Th r cgc Arg 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 WO 01/00805 ,PCT/IBOO/00926 gcg gga tat ggt gca aca atc gca acq att ttg ttc cig ttq ctq ctg 979 Ala Gly Tyr Gly Ala Thr Ile Ala Thr Ile Leu Phe Leu Leu Leu Leu 280 285 290 att atc act gtt atc cag qtt cga tac atg gat aag gag aac aag cag 1027 Ilie Ilie Thr Val Ile Gin Val Arg Tyr Met Asp Lys Giu Asn Lys Gin 295 300 305 aaa tgatctcgac tgatagaaac gtt 1053 Lys 310 <210> 662 <211> 310 <212> PRT <213> Corynebacterium glutamicun <400> 662 Val Ser Thr Leu Ile Ser Giu Pro Giu Vai Asp Lys Leu Arg Lys Arq 1 5 10 Ala Lys Arg Ser Arg Arg Thr Giu Trp Trp Leu Ala Ala Ala L *eu Leu 25 Ala Pro Asn Leu Leu Leu Leu Ala Ilie Phe Thr Tyr Arg Pro Leu Leu 40 Asp Asn Phe Arg Leu Ser Phe Phe Asn Trp Asn Ile Ser Ser Pro Thr 55 Ser Thr Phe Ilie Gly Phe Asp Asn Tyr Val Giu Phe Phe Thr Arg Ser 70 75 Asp Thr Leu Gin Val Val Leu Asn Thr Val Ilie Phe Thr Ala Cys Ala 90 Val Ile Gly Ser Met Val Leu Gly Leu Leu Leu Ala Met Leu Leu Asp 100 105 110 Gin Lys Leu Phe Gly Arg Asn Phe Val Arg Ser Met Val Phe Ala Pro 115 120 125 Phe Val Ilie Ser Gly Ala Ala Ile Gly Val Ala Phe Gin Phe Val Phe 130 135 140 Asp Pro Asn Phe Gly Leu Val Gin Asp Leu Leu Gly Ar Ile Gly Val 145 150 155 160 Asp Ser Pro Gin Phe Tyr Gin Asn Pro Asn Trp Ala Leu Phe Met Val 165 170 175 Thr Phe Thr Phe Val Trp Lys Asn Leu Gly Tyr Ser Phe Val Ile Tyr 180 185 190 Leu Ala Ala Leu Gin Gly Leu Asn Lys Asp Leu Ser Glu Ala Ala Pro 195 200 205 Val Asp Gly Ala Ser Ala Trp Thr Arg Phe Trp Lys Val Thr Leu Pro 210 215 220 WO 01/00805 Gin Leu Arg Pro Thr Thr Phe Phe Leu Ser Ile 225 230 235 Ser Val Gin Vai Phe Asp Ile Ile His Thr Met 245 250 Leu Gly Asn Gly Thr Thr Thr Leu Val Tyr Gin 260 265 Phe Thr Asn Tyr Arg Ala Giy Tyr Gly Ala Thr 275 280 Phe Leu Leu Leu Leu Ile Ilie Thr Val Ile Gin 290 295 Lys Glu Asn Lys Gin Lys 305 310 <210> 663 <211> ii82 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (1159) <223> RXA01737 <400> 663 ctttgaqccg aacgatctgc cgactcaaga agaactagcc atgcgcctaa gatcaaacaa gaatqaaagg acqqtqaaca Th r Thr Val1 Ile Val 300 PCT/IBOO/00926 Val Thr Leu Asn 240 Arq Gly Gly Pro 255 Tyr Thr Glu Thr 270 Ala Thr Ile Leu 285 Arg Tyr Met Asp qg t Gly atc Ile atc Ile ttt Phe tac Tyr C Cq Pro ga a Giu ccg Pro gga Gly gac Asp tat Tyr gtq Val gat Asp gcg Al a atg Met gga Giy gtc Val tcg Se r ctg Le u aaa Lys gtq Val1 45 tac Tyr t cg Se r gca Al a cgqctqctca gtcagqaatg atg gqc cga atq aaa Met Giy Arg Met Lys 1 gca ctt ttg agc att Ala Leu Leu Ser Ile tgq gcg ctg atc tta Trp Ala Leu Ile Leu atc gtc acc atg gtt Ile Val Thr Met Val ctc acg ttc atc gac Leu Thr Phe Ile Asp gtqgqgc tac ggc qat Val Gly Tyr Giy Asp aac atc atc gtc ctc Asn Ile Ile Val Leu 100 115 163 211 259 307 355 403 WO 01/00805 WO 0100805PCTIBOOIOO926 acc Cca Thr Pro.

tca gtg Ser Val aga aaa Arg Lys 135 ggt cgc Gly Arg 150 cag atc Gin Ilie aac aqc Asn Ser ttg cgt Leu Arg aac ctg Asn Leu 215 gcg ccg Aia Pro 230 cac ctc His Leu acc gct Thr Aia gag atg Giu Met gaa cga Giu Arg 295 gct gac Aia Asp 310 gac tcc Asp Ser gca Aila ct C Le u 120 cgc Ar g tcc Ser gtt Val gg a Giy cta Le u 200 ga c Asp cag Gin ctc Le u gg C Giy atg Met 280 cta Le u atc Ile cca Pro cgc Arg 105 acc Thr atg Met g cg Ala gtt Vali ct C Le u 185 gct Ala qa t Asp gca Ala ga a Glu cg a Arg 265 ga a Glu gtc Val1 gtc Val1 ga a Giu atc Ile gaa Glu cg C Arg gtc Val1 atc Ile 170 gtc Val1 Giy act Th r atg Met caa Gin 250 a tg Met gac Asp gg t Gi y ctc Leu gca Ala ggc ttc Gly Phe gaa tcg Giu Ser aac cac Asn His 140 gct gca Ala Ala 155 gac acc Asp Thr acc gtc Thr Val qtg tca Val Ser gca gtt Ala Val 220 att gtg Ile Val 235 tcc ggt Ser Gly ctc ggt Leu Gly ctc ctc Leu Leu gag gat Glu Asp 300 ggt gtt Gly Val 315 gaa act Giu Thr ct g Leu cgc Arg 125 acc Thr ctg Le u gat Asp aaa Lys cg a Arg 205 ctg Le u gcc Ala g cg Ala ctg Leu tca Ser 285 gaa Glu gtt Val1 gta Val1 atc Ile 110 cgg Arg gt C ValI ctt Le u caa Gin Gi y 190 gcg Ala gtg Val1 aqt Ser gac Asp g ca Ala 270

CCC

Pro atc Ile cga Arg gag Glu ctc Leu gcc Ala gtt Val ggc Gly gtc Val1 175 tcC Ser cgc Arg act Thr gt C Val1 t cg Ser 255 aca Th r gac Asp ggc Gly tcc Ser

CCC

Pro 335 aat ctg Le u ctg Le u gtc Val ga c Asp 160 tcc Ser gcc Al a gcc Ala cta Leu cg a Arg 240 gtt Val gt t Val1 ga a Glu tCC Ser ggt Gly 320 ggc Gly gtc ValI caa Gin gga Gly 145 ggc Gly ctc Leu acc Thr gtc Val1 t cg Se r 225 gaa Glu gtg Val1 acc Th r gga Gly aac Asn 305 gag Giu gat Asp ggc Gly atc Ile 130 tac Tyr gtc ValI ga C Asp aaa Lys gtc Val1 210 gtg Val1 tct Ser atc Ile cc a Pro ttc Phe 290 cca Pro ctc Le u cg t acc Th r 115 ca a Gin gg a Gly ccc Pro gcc Al a gca Ala 195 g tg Val cg a Arg ga a Glu t cc Ser t cg Ser 275 tcc Ser cg a Arg tac Tyr ctc acc Thr cg t Arg a cc Thr gcc Ala gcc Ala 180 gat Asp gca Ala gaa Giu aac Asn tca Ser 260 gtt Val1 gt t Val1 cac His cgc Arg ctt ttg Leu t gg Trp aaa Lys aac Asn 165 aac As n gtg Val ccg Pro atc Ilie caa Gin 245 ga a Glu gtg Val gcc Ala ctc Leu atc Ile 325 tac 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1169 Arg Leu Leu Tyr 330 gtt cgc cga gta ttt agc gag gag gta gac aaa tgagaattct WO 01/00805 Val Arg Arg Val Phe Ser Glu Glu Val Asn Asp Lys 345 350 tcccatcggc CCC <210> 664 <211> 353 <212> PRT <213> Corynebacterium qiutamicun PCTIIBOO/00926 1182 <400> 664 Met Gly Arg Met Lys Asn Asp Gly Glu 1 Ala T rp Ile Leu Val1 Asn Val1 Gin Gly 145 Gly Leu Thr Val1 Ser 225 Glu Leu Ala Val1 Th r Gi y Ilie Gly Ile 130 Tyr Val1 Asp Lys Val1 210 Val Ser Leu Leu Thr Phe T yr Ile Th r 115 Gin Gly Pro Ala Al a 195 Val Arg Glu Se r Ile Met Ile Gly Val1 100 Thr Arg Th r Ala Ala 180 Asp Ala Glu Asn 5 Ile Leu Val1 Asp Asp Le u Leu Trp Lys Asn 165 Asn Val1 Pro Ile Gin Arg Arg Tyr 55 Le u Thr Pro Val Lys 135 Arg Ile Ser Arq Leu 215 Pro Le u Ile Ile 40 Ph e Tyr Pro Ala Le u 120 Arg Ser ValI Gly Le u 200 Asp Gin Leui Pro 25 Gly Asp T yr Val1 Arg 105 Thr Met Ala Val Le u 185 Al a Asp Al a Glu Le u 10 Gin T yr Arg Ser T hr 90 Ile Giu Arg ValI Ile 170 ValI Gl y Th r Met Gin 250 Met Asp Ala Met Gly Val1 Ser Phe Se r His 140 Ala Th r Val1 Se r Val1 220 Val Gly Leu Lys ValI Tyr Ser Ala Leu Arg 125 Thr Leu Asp Lys Arg 205 Le u Ala Ala Pro Arg Le u Se r Le u Arg Ile 110 Arg Val1 Le u Gin Gly 190 Al a Val Ser Asp Asp Ser Leu Giu Thr Le u Leu Ala Val1 Gly ValI 175 Ser A rg Thr Val Se r 255 His Pro Val1 Asp Th r Ile Le u Leu ValI Asp 160 Ser Ala Al a Le u Arg 240 Val 245 Val Ile Ser Ser Glu Thr Ala Gly Arg e l e l h a Leu Gly Leu Ala Thr Val Thr Gly Asn 305 Giu Asp Lys WO 01/00805 260 Pro Ser Val 275 Phe Ser Val 290 Pro Arg His Leu Tyr Arq Arg Leu Leu 340 Val Giu Met Met 280 Ala Giu Arq Leu 295 Leu Ala Asp Ile 310 Ilie Asp Ser Pro 325 Tyr Val Arg Arg Asp Leu Leu Ser 285 Gly Glu Asp Giu 300 Leu Gly Val Val 315 Ala Glu Thr Val 330 Phe Ser Glu Glu PCT[IBOO/00926 270 Pro Ile Arg Gi u Val1 350 <210> 665 <211> 2007 <212> DNA <213> Corynebacterium qiutamicum <220> <221> CDS <222> (1984) <223> RXN02348 <400> 665 aaagacccga qccgaagccc tqqcctgcgc atacttcctt ggtcttttct gagcgctagc atttctccac tcaaaggagc aaa Lys ct C Leu ccc Pro g aa Giu acc Thr qct Ala ag t Ser act Thr ctq Leu gtc Vai at c Ile gac Asp cca Pro gcc Ala ctt Le u gaa Giu atc Ile ga Gly aaa Lys cta.

Leu cac His aat Asn gtc Val 75 caa Gin tca Ser ggt Gly act Th r gt q ValI 60 acc Th r gqt Gly gct Ala gtg Val1 ttc Phe ggC Giy aaa Lys atc Ile aaa Ly s gg C Gi y atg Met atc Ile gtc Val1 80 qcg Aila qtcaacqctc gctggqatta atgj ctt aac cqc atq 115 Met Leu Asn Arq Met 1 tcc gqa caa gct tta 163 Ser Gly Gin Ala Leu gac atc ggc acc agjc 211 Asp Ile Giy Thr Ser cag cac aac aaa gtc 259 Gin His Asn Lys Val tcc atg gtg ttg tgg 307 Ser Met Val Leu Trp atg ctg gtc acc cga 355 Met Leu Val Thr Arg ctc qtt gct ttq ctg 403 Leu Vai Ala Leu Leu 100 wo 01/00805 WO 0100805PCTIIBOO/00926 aaa Ly s ggC Gly gtt Val1 cgc Arq 150 atc Ile atq Met atc Ile cgc Arg gtt Val 230 cat His cct Pro cag Gin ctg Leu tcg Ser 310 aac As n gcc Al a ctc Le u 135 ttc Phe caa Gin t tg Leu ggq Gly ttg Le u 215 gt c Vai ttt Phe gct Aia cct Pro C9g Arg 295 cag Gin cg t Arg gca Ala 120 agc Ser att Ile c cg Pro ct g Leu cac His 200 att Ile ct g Le u g qq Giy tta Leu qa a Giu 280 att Ilie gcc Aila ggg Gly 105 ttg Le u gca Aila ct g Leu ctc Le u tgg Trp 185 cca Pro gtg Val1 aca Thr g cg Ala atc Ile 265 gcq Al a ccg Pro gtg Val1 cac His ttt Phe a ca Th r

CCC

Pro gt Giy 170 ttt Phe ga a Giu gct Ala qt a Val1 agg Arg 250 ttg Le u gtg Vali ttg Leu att Ile tgg qqa Trp Giy tat ggc Tyr Gly gaa ggc Giu Gly 140 gta tct Val Ser 155 aca gaa Thr Giu gtc acc Vai Thr atc ttg Ilie Leu gag cct Giu Pro 220 acg ggt Thr Gly 235 cca atc Pro Ilie acq tat Thr Tyr cgc aac Arg Asn gtt att Val Ilie 300 tct ggt Ser Giy 315 aaa Lys qat Asp 125 ttg Leu ctc Leu aaa L ys ctt Le u cag Gin 205 ttc P he qcg Ala aqa Arg ttg Le u ccc Pro 285 ttq Leu gcg Aia ttc Phe 110 gt q Val acg Thr gca Ala gtc Vali gca Aia 190 agc Ser caa Gin gaa Giu gtg Val 9g9 Gly 270 atg Met g cg Ala tat Tyr gtg Val qtg Vai gt t Val gtt Vali qgc Gly 175 gga Giy ttq Leu g ca Ala 9g Aila g cg Al a 255 cag Gin ttt Phe acc Thr tca Ser gca Ala atc Ile atc Ile ctg Leu 160 aaa Lys t tg Leu tct Ser ttt Phe ct C Leu 240 tgg Trp ggc Giy tat Tyr atc Ile ttg Leu 320 gta Vali acc Th r tcc Se r 145 atc Ile gcc Al a gga Gly cca Pro qtq Vai 225 tac Tyr ttt Phe gcc Ala ctc Le u gct Aia 305 acc Thr qcc Aia C cg Pro 130 cca Pro gct Ala ttc Phe att Ilie cat His 210 ct g Leu gct Ala tgc Cys ttg Leu g cg Ala 290 acg Thr aaq Lys gqc Gly 115 qcg Ala agc Ser att Ilie Giy ccg Pro 195 tgg Trp Ct t Le u ga t Asp gtc Val1 qt q Val 275 ccg Pro gt g Val1 ca g Gin atg Met atc Ilie tt t Phe ttt Ph e ccc Pro 180 caa Gin gcc Ala ggt Giy atg Met gtc Val 260 atc Ile gaa Giu atc Ile gcc Aila ttg Le u tct Se r gag Giu qca Ala 165 atc Ile atc Ile ctg Leu g cc Al a ggc Gly 245 atq Met aac Asn gg t Gly gca Al a gtg Vai 325 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 aat ttg aaa ctg ctg cca cgc atq gtg atc cgg cat acc tcc cgc aaa Asn Leu Lys Leu Leu Pro Arg Met Val Ile Arg His Thr Ser Arg Lys 330 335 340 gag Giu tcc Ser agc Ser ctg Leu 390 att Ilie acg Thr ctc Leu cag Gin Ct C Leu 470 gta Val1 igc Cys atc Ilie atg Met cac His 550 att Ile WO 01/00805 gaa ggc cag atc Giu Gly Gin Ile 345 gtg atg gtt gig Val Met Val Val 360 gcg tac gga ctt Ala Tyr Gly Leu 375 tat cig atc tat Tyr Leu Ile Tyr gig cic aic ggt Vai Leu Ilie Giy 410 aaa att cac gac Lys Ile His Asp 425 atc gig gtg atg Ilie Vai Vai Met 440 gaa cgc gca gag Giu Arg Ala Glu 455 gat cag cca cac Asp Gin Pro His itt cca cat ggc Phe Pro His Gly 490 gtg aaa gac cic Vai Lys Asp Leu 505 gic caa gaa cac Vai Gin Giu His 520 qaa gtg ctc cat Giu Vai Leu His 535 cit ggt tat ttt Leu Giy Tyr Phe gac cca aca tgg Asp Pro Thr Trp tat atg cca ctg gtt, Tyr Met Pro Leu Vai 350 gtg ctg gta ttc cga Vai Leu Vai Phe Arg 365 gca gig acc gga acc Ala Val Thr Gly Thr 380 gtt cac acc aca tg Vai His Thr Thr Trp 395 att cca gaa gta ct Ile Pro Giu Vai Leu 415 ggt ggc tgg cit cca Giy Gly Trp Leu Pro 430 cgg acc tgg gag tgg Arg Thr Trp Giu Trp 445 cig gaa ctt ccc aig Leu Giu Leu Pro Met 460 aat ait ggt cig cgt Asn Ilie Giy Leu Arg 475 acc agc gat act gtc Thr Ser Asp Thr Vai 495 aag ctt ita tac cga Lys Leu Leu Tyr Arg 510 git ccg cac gig cca Vai Pro His Val Pro 525 cac gcc ccg atc aga His Ala Pro Ile Arg 540 gat gag cag aac ctg Asp Giu Gin Asn Leu 555 gat aac gcc acc tac Asp Asn Aia Thr Tyr 575 cct gga aag ait gct aai As n t cc Ser tig Leu t gg Trp 400 ct a Leu cta Le u gga Giy gat Asp aaa Ly s 480 ccg Pro gag Giu cca Pro gt c Val1 cci Pro 560 itc Phe g c r gga Giy ict Ser gig Val1 385 aaa Lys5 tc Phe ctt Leu agt Se r aag Ly s 465 gt Val1 ttg Le u atc Ile gag Giu gig Val1 545 gag Giu ctg Le u i 99 ttg Leu gaa Giu 370 cig Leu aca Thr gcc Ala at Ile gac Asp 450 ttc Phe gcc Al a ica Se r gig Val gaa Giu 530 cg a Arg cat His ici Se r cg i ctg Le u 355 agc Ser gi c Val1 9 cg Ala t cg Ser 9 cg Ala 435 cgc Arg ttg Leu gaa Giu ttg Le u atc Ile 515 cgc Arg gt Val1 ctc Le u gcc Ala ga t tt Phe ctc Le u agc Ser ct 9 Leu aac Asn 420 gcc Ala gi c Val1 gag Glu gig Val gtt Val1 500 gii Val1 gcg Ala gat Asp cat His cig Le u 580 cg i

PC

gia Val1 gcc Ala gic Val ttc Phe 405 acc Thr gig Val aat Asn aaa Lys gca Al a 485 cgc Arg cga Arg gaa Giu cig Leu gc c Ala 565 aci Th r iig T11BO0100926 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 cti cgg agc 570 agg tig WO 01/00805 Leu Arg Ser Arq Leu Pro Gly Lys Ile 585 590 tat ctt tcg atg qaa cgt aat cag gca Tyr Leu Ser Met Giu Arq Asn Gin Ala 600 605 ttg caa cca agc aaa acc atc acg gtt Leu Gin Pro Ser Lys Thr Ile Thr Vai 615 620 taatcaggca gttgctggcc aac <210> 666 <211> 628 <212> PRT <213> Corynebacteriun giutamicum <400> 666 Met Leu Asn Arg Met Lys Ser Ala Arg PCTIBOO/00926 Aia Giy Trp Arg Asp Arg Leu 595 tct cga act gag tct ttc aaa Ser Arg Thr Giu Ser Phe Lys 610 gga aca gag ctg cac ctt Giy Thr Giu Leu His Leu 625 1939 1984 2007 i Se r Asp Gin Ser Met Le u Vali Thr Se r 145 Ile Aila Giy Pro Gin Gly Asn Vali Vali Ala Gly 115 Ala Ser Ile Gi y Pro 195 T rp Ala Th r Lys Le u Th r Leu 100 Met Ilie Phe Phe Pro 180 Gin Ala 5 Leu Ser Vali T rp Arg Leu Le u Ser Giu Ala 165 Ile Ile Leu Leu Pro Giu Thr 70 Al a Lys Gly Val1 Arg 150 Ilie Met Ile Arg Thr Le u Val1 Ile Asp Asn Ala Le u 135 P he Gin Leu Gly Leu 215 Leu Tyr 40 Thr Thr Asn Arg Al a 120 Se r Ile Pro Leu His 2U0.

Ile Gi y 25 Se r Gin Leu Gin Gly 105 Leu Ala Leu Leu Trp 185 Pro Val1 Pro 10 Ala Le u Giu Ile Giy 90 His Phe Thr Pro Giy 170 Ph e Glu Ala Lys Leu His Asn Val1 75 Gin T rp Tyr Giu Val 155 Thr Val Ile Glu Ser Gi y Th r Val1 Thr Gi y Ci y Gl y Cly 140 Ser G iu Thr Leu Pro 220 Val Val Ala Tyr Val1 Gi y Lys Asp 125 Leu Leu Lys Leu Gin 205 Ph e Ala Pro Val Phe Phe Ser Cly Ile Lys Tyr Ile Leu Phe Val 110 Val Val Thr Val Ala Vai Val Gly 175 Ala Gly 190 Ser Leu Gin Ala Lys Gi y Met Ile Val1 Al a Ala Ile Ile Le u 160 Lys Leu Se r Phe WO 01/00805 WO 0100805PCTJIBOO/00926 Val1 225 Tyr Ph e Ala Leu Al a 305 Th r His Gl y Ser Val1 385 Lys Phe Le u Se r Lys 465 Val Le u Ile G lu Leu Ala Cys Leu Al a 290 Th r Lys Thr Leu Glu 370 Le u Thr Ala Ile Asp 450 Phe Al a Ser Val1 Glu 530 Leu Asp Val1 Val1 275 Pro Val1 Gin Ser Le u 355 Ser Val1 Al a Ser Ala 435 Arg Le u Glu Le u Ile 515 Arg Gly Met Val1 260 Ile Glu Ile Ala Arg 340 Phe Leu Ser Le u Asn 420 Al a Val1 Glu ValI Val1 500 Val1 Ala Ala Gl y 245 Met As n Gly Al a Val1 325 Ly s Val1 Ala Val1 Phe 405 Th r Val1 Asn Lys Ala 485 Arg Arg G lu Val1 230 His Pro Gin Le u Se r 310 Asn Gl u Se r Se r Le u 390 Ile Th r Leu Gln Le u 47 0 ValI Cys Ile Met Val1 Ph e Ala Pro Arg 295 Gln Leu G lu Val1 Al a 375 Tyr Val Lys Ile Gi u 455 Asp Phe Val1 Val1 Gi u 535 Leu Gi y Le u Gi u 280 Ile Ala Lys Gly Met 360 Tyr Le u Le u Ile Val1 440 Arg Gln Pro Lys Gln 520 ValI Thr Ala Ile 265 Ala Pro Val Leu Gln 345 Val Gly Ile Ile His 425 Val Al a Pro His Asp 505 Giu Leu Val Arg 250 Leu Val1 Leu Ile Leu 330 Ile Val Leu Tyr Gly 410 Asp Met Glu His Gly 490 Le u His His Thr 235 Pro Thr Arq Val1 Ser 315 Pro Tyr Val Ala Val 395 Ile Gly Arg Leu Asn 475 Thr Lys Val1 His Gly Ile T yr Asn Ile 300 Gi y Arg Met Leu Val 380 H is Pro Gly Th r Glu 460 Ile Se r Le u Pro Ala 540 Ala Arg Leu Pro 285 Leu Al a Met Pro ValI 365 Th r Thr Giu Trp Trp 445 Le u Gly Asp Leu His 525 Pro Glu Val1 Gly 270 Met Ala Tyr Val Le u 350 Ph e Gi y Thr Val1 Le u 430 Glu Pro Le u Thr Tyr 510 Vai Ile Ala Ala 255 Gin Phe Thr Ser Ile 335 Val Arg Thr T rp Leu 415 Pro Trp Met Arg Val 495 Ar g Pro Arq Le u 240 Trp Gly Tyr Ile Le u 320 Arg Asn Ser Le u T rp 400 Le u Le u Gly Asp Lys 480 Pro Glu Pro ValI WO 01/00805 Vai Arg Val Asp Leu His Leu Gly Tyr Phe Asp 545 550 555 Giu His Leu His Ala Ile Asp Pro Thr Trp Asp 565 570 Leu Ser Ala Leu Thr Leu Arg Ser Arg Leu Pro 580 585 Trp Arg Asp Arq Leu Tyr Leu Ser Met Glu Arq 595 600 Thr Glu Ser Phe Lys Leu Gin Pro Ser Lys Thr 610 615 Giu Leu His Leu 625 <210> 667 <211> 1656 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1633) <223> RXA02426 <400> 667 tgcagcagca taatcaqqga aaaataggaa ctqtttactg Glu Gin Asn Leu Asn Ala Thr Tyr 5'75 Gly Lys Ile Ala 590 Asn Gin Ala Ser 605 Ile Thr Val Gly 620 PCTJIBOO/00926 Pro 560 Phe Gi y Arg Th r aaaaactgac aaacctgaca gacctgaccc qaaqaqacct tattcatgac qatattgttc atg ctc att aca ttg Met Leu Ile Thr Leu acc Thr qct Al a ccg Pro ccg Pro t cg Se r acc Th r ggt gtq Val ttq Leu ga a Gi u ctg Leu cag Gin atc Ilie ctt att Ile at g Met ttt Phe ct g Leu atq Met qcg Ala gct gtg Vali att Ile atc Ile gcg Al a acc Thr ctc Le u 9c9 gCC Ala gtc Val1 30 Ccg Pro ctg Leu atc Ile acg Th r atc atc Ile 15 qcc Ala gcc Ala gcg Al a atc Ile gg t Gly 95 atg 9Cc Gi y gca Ala ga t Asp a gg Arg acg Thr 80 gca Ala ttg 1 gat Asp ggt Gly ctg Leu aag Lys atg Met tCC Ser gc t aaa Ly s gga Gly atg Met tca Se r t cg Ser atq Met gcg acc Thr gc9 Ala ttg Leu t cg Ser gtg Val ctq Le u gct gga Gi y ttg Le u cct Pro t gg Trp ttg Le u ttg Le u 100 at t ct C Leu ttg Le u att Ile gcg Ala t tg Leu Ctt Le u qc t 115 163 211 259 307 355 403 451 WO 01/00805 WO 0100805PCTA[BOO0926 Pro Gly Ile cca Pro ccg Pro gca Al a 150 ggt Gly t gt Cys t gg T rp t gg Trp t cg Se r 230 gcg Ala acc Thr aat As n gtg Val gitg9 Val 310 aag Lys t cg Ser ccg Pro aag Lys 135 9C C Ala gag Glu ct t Leu ttt Phe gtg ValI 215 ggc.

Gly t cgj Ser att Ile gtg Val gtc Val1 295 t gg Trp ggt Gly ga t Asp 120 cg C Arg agc Ser gat Asp gct Al a acc Thr 200 ctg Leu gtt Val att Ile gag Giu cgc Arg 280 gt g Val1 atg Met gcg Ala Gly 105 cct Pro at C Ile atC Ile ttg Le u gc c Al a 185 ga t Asp ccg Pro atc Ile ggt Gly gtg Val 265 gct Al a gg C Gly ttc Phe acc Thr gt t Val1 acc Th r gt g ValI tcc Se r 170 gt t Val1 cac His ttt Phe gcc Ala qcg Al a 250 ctg Leu gcg Ala att Ile g cg Ala aat As n 330 gc t Ala acc Th r gcg Ala 155 tgg Trp att Ile gtg Val1 gcc Ala att Ile 235 gag Glu ttt Phe att Ile gtg Val1 gcg Al a 315 ag t Se r gtt ValI act Th r 140 ttc Ph e t ca Se r t tgC Leu agt Ser att Ile 220 gt g Val gat Asp act Th r gat Asp ctc Leu 300 tat T yr tca Se r ga t Asp 125 ttg Le u cat His acg Th r 9 9C Gly tcc Se r 205 tat Tyr atc Ilie cgt Arg g gc Gly gaa Glu 285 t cg Ser aag Lys ttg Le u 110 gca Ala cag Gln gt 9 Val1 g9t Gly ttg Le u 190 gt t Val1 gtg Val gct Ala cta Le u gt t Val1 270 gt t Val gt 9 ValI cgc Arg Cgg Arg gtg Val1 a cq Th r gct Al a gt t Val1 175 gtt Val1 gag Glu gtg Val1 gca Al a acg Thr 255 9CC Al a 99 a Gly gtg Val1 aa t As n gCg Ala 335 cg C Ar 9 gcg Ala gag Glu tta Le u 160 ttg Leu att Ile gc t Al a gcg Al a gt g Val 240 ggt Gly ttt Phe tct Se r g9 Ala cg t Arq 320 CC t Pro gaa Gi u 99 t Gly 145 gct Al a ga a Glu ggc Gly cg a Arg gaa Gi u 225 gag Gi u tCt Se r ggt Gi y gag Glu atc Ile 305 ttc Phe ctt Leu cct Pro 130 ctg Le u 9CC Ala ttc Phe C gg Arg aat Asn 210 g aa Glu atg Met 9 C9 Ala ttg Leu Ctg Leu 290 gtt Val a ag Lys cga Arg 115 gcg Ala ttt Phe Ctg Leu ttg Leu g9 Al a 195 g9 Al a atc Ile aat As n ttc Phe atc Ile 275 tgg Trp gtc Val1 atc Ile Ctg Le u Leu Ala Gly Ala Ilie Met Leu Ala Ala Ala Ile Ala gga Gly aat Asn gtc ValI t 99 Trp 180 gct Al a ttt Ph e qqC Gly t Cg Se r tgg Trp 260 9gci Gly cat His cgt Arg gat Asp c a g Gin 340 atc Ile gat Asp gct Ala 165 t cg Ser g C g Ala acg Thr gga Gly a 99 Arg 245 gga Gi y Ctg Le u 9CC Al a ggt Gly aa g Ly s 325 gaa Glu gcg Ala 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 ttg ctg atq acq tgg gcg ggc atg Leu Leu Met Thr Trp Ala Gly Met ggt ttg gtg acg ttg Gly Leu Val Thr Leu WO 01/00805 PCT/IBOO/00926 gtg Val gtC Va1 375 acg Thr gct Ala gcc Ala atg Met ctg Leu 455 gtc Va1 gag Glu ccg Pro gtc Va1 ctg Leu 395 gag Glu tat Tyr aag Lys gtc Va1 aag Lys 475 aat Asn gag Glu gtt Val 380 atg Met agt Ser ttg Leu aat Asn gat Asp 460 gcg Ala ccg Pro gat Asp 365 ctc Leu Cgg Arg atc Ile gtg Va1 tgg Trp 445 aag Lys gcc Ala gcc Ala ttc Phe atc Ile ctc Leu gcg Ala 415 acc Thr tcg Ser cat His gag Glu gtt Va1 495 ccg Pro acc Thr agc Ser 400 ctg Leu acg Thr caa Gin cag Gin gag Glu 480 gat tat Tyr atg Met 385 ttg Leu aca Thr gag Glu gaa Glu Cgc Arg 465 ctg Leu gag cac His 370 gtg Val gat Asp gag Glu ttg Leu att Ile 450 gcg Ala ttg Leu gtg cac His ggc Gly aag Lys agg Arg ccg Pro 435 gac Asp cat His aag Lys ttg gag Glu cct Pro ggt Gly gct Ala 420 atg Met gct Ala cat His gcg Ala acc ttg Leu ggt Gly ccc Pro 405 cac His gag Glu gat Asp g cg Ala Cgg Arg 485 aac 1219 1267 1315 1363 1411 1459 1507 1555 1603 1653 1656 Asp Giu Val Leu Thr Asn 500 tagataaaca gcagcaggct att gac cgg atg ctt gtt gcg gct gaa cgc Ile Asp Arg Met Leu Val Ala Ala Giu Arg 505 510 gat <210> 668 <211> 511 <212> PRT <213> Corynebacterium glutamicum <400> 668 Met Leu Ile Thr Leu Leu Leu Ala Thr Val 1 5 10 Asp Lys Thr Gly Leu Pro Trp Pro Ala Leu 25 Gly Gly Aia Leu Leu Pro Phe Leu Pro Glu 40 Leu Met Leu Pro Ile Phe Ile Pro Pro Leu Ile Val Val Ala Ile Gly Met Thr Ile Val Ala Ala Phe Thr Ile Pro Ala Asp Leu Trp Ala Leu Ala Arg WO 01/00805 PCT/IB00/00926 55 Lys Ser Ser Trp Ala Val Ile Arg Ser Gin Met Ser Thr Ile Ile Thr 70 75 Met Ser Val Leu Leu Val Phe Val Thr Ile Ala Ala Leu Thr Gly Ala 90 Ser Met Leu Leu Leu Pro Gly Ile Gly Leu Ala Gly Ala Ile Met Leu 100 105 110 Ala Ala Ala Ile Ala Pro Pro Asp Pro Val Ala Val Asp Ala Val Ala 115 120 125 Glu Pro Ala Gly Ile Pro Lys Arg Ile Thr Thr Thr Leu Gin Thr Glu 130 135 140 Gly Leu Phe Asn Asp Ala Ala Ser Ile Val Ala Phe His Val Ala Leu 145 150 155 160 Ala Ala Leu Val Ala Gly Glu Asp Leu Ser Trp Ser Thr Gly Val Leu 165 170 175 Glu Phe Leu Trp Ser Cys Leu Ala Ala Val Ile Leu Gly Leu Val Ile 180 185 190 Gly Arg Ala Ala Ala Trp Phe Thr Asp His Val Ser Ser Val Glu Ala 195 200 205 Arg Asn Ala Phe Thr Trp Val Leu Pro Phe Ala Ile Tyr Val Val Ala 210 215 220 Glu Glu Ile Gly Gly Ser Gly Val Ile Ala Ile Val Ile Ala Ala Val 225 230 235 240 Glu Met Asn Ser Arg Ala Ser Ile Gly Ala Glu Asp Arg Leu Thr Gly 245 250 255 Ser Ala Phe Trp Gly Thr Ile Glu Val Leu Phe Thr Gly Val Ala Phe 260 265 270 Gly Leu Ile Gly Leu Asn Val Arg Ala Ala Ile Asp Glu Val Gly Ser 275 280 285 Glu Leu Trp His Ala Val Val Val Gly Ile Val Leu Ser Val Val Ala 290 295 300 Ile Val Val Arg Gly Val Trp Met Phe Ala Ala Tyr Lys Arg Asn Arg 305 310 315 320 Phe Lys Ile Asp Lys Lys Gly Ala Thr Asn Ser Ser Leu Arg Ala Pro 325 330 335 Leu Arg Leu Gin Glu Ser Leu Leu Met Thr Trp Ala Gly Met Arg Gly 340 345 350 Leu Val Thr Leu Ala Leu Val Leu Ser Ile Pro Glu Asp Ile Phe Pro 355 360 365 Tyr His His Glu Leu Gin Val Ile Ala Leu Val Val Leu Leu Ile Thr 370 375 380 Met 385 Le u Thr Giu Glu Arq 465 Le u Glu WO 01/00805 Val GIly Pro Asp Lys Gly Glu Arg Ala 420 Leu Pro Met 435 Ile Asp Ala 450 Ala His His Leu Lys Ala Val Leu Thr 500 PCT/IBOO/00926 Gly Pro 405 His Glu Asp Ala Arg 485 As n Le u 390 Asp Lys Gin Glu Arg 470 Arg Ilie Thr Al a Ala Met Le u 455 Val1 Glu Asp Le u 395 Glu Tyr Lys Val Lys 475 As n Val1 Met Ser Leu Asn Asp 460 Al a Pro Ala Leu Ala 415 Thr Ser His Giu Val1 495 Arg <210> 669 <211> 993 <212> DNA <213> Corynebacteriun giutarnicum <220> <221> CDS <222> (101)..(970) <223> RXN03164 <400> 669 cttttttgca tccagatgca caaagccgtg gcacaaacga tgtcatqqca tatcaaccag cagacaatcg ctatgacgac gtg Val cac His cac His tat Tyr cg t Arg aat Asn ttc Phe gcg Al a cct Pro ga t Asp tct S er gg t Gly ttt Phe cca Pro ctc Leu ct t Leu ccg Pro gtc Val1 45 gca Ala cgc Arg gacaaactqa gcacaatggc atg atc tac cgc agg met Ile Tyr Arg Arg 1 tcg ctt ggg ctg tgg Ser Leu Gly Leu Trp cag cgc agc att att Gin Arg Ser Ile Ile gat ttg gct aat aac Asp Leu Ala Asn Asn ttt ggc agg att ttq Phe Gly Ar Ile Leu atc att tct tcc aag Ile Ile Ser Ser Lys WO 01/00805 WO 0100805PCTf[BOOOO926 gcg ggt tgg Ala Gly Trp aag tat cta Lys Tyr Leu gat tac gtg Asp Tyr Val 120 ttg gaa gaa Leu Glu Giu 135 gct ctt tac Ala Leu Tyr 150 gcg gct gag Ala Ala Giu cca agc tat Pro Ser Tyr ggt gag aac Gly Giu Asn 200 gct ttc tca Ala Phe Ser 215 gga att cca Gly Ile Pro 230 ggc atg ttg Gly Met Leu atc gcc cag Ile Ala Gin gtg ctg cgc Val Leu Arg 280 ga t Asp gtg Val 105 ga t Asp acc Thr gt g Val1 ttc Phe t cc Se r 185 ttg Le u cca Pro gag Glu aac Asn gaa Glu 265 gag Giu gga Gly ga t Asp cat His 125 t tg Leu t cc Ser gag Glu cgt Arg gct Ala 205 ggc Gly gcc Ala at t Ile tca Ser cc t Pro cag Gin 110 ca c His cgt Arg tac Tyr gg C Gly t gg Trp 190 g cc Al a ct g Leu agc Ser gat Asp ctt Le u 270 tat Tyr tcc S er cgc Arg ga c Asp ggt Gi y tgc Cys 175 gtg Val1 a ac Asn ctc Le u c ag Gin atg Met 255 g cg Ala 80 gg t Gly ct g Le u ccg Pro att Ile cca Pro 160 ccg Pro gag Gi u aat Asn a cg Thr ggt Gly 240 gt c Val1 cag Gin gg t Gly cg c Arg cca Pro 130 geg Al a ctc Leu ctg Le u ccg Pro ctt Leu 210 aaa Lys t CC Se r a ag Lys gcg Ala gg t Gly ctc Le u 115 ga t Asp tct Se r aca Thr att Ile gg C Gi y 195 9gC Gly tat T yr ct g Le u ctc Leu ctt Leu 275 tcc Ser 100 ggc Gly act Thr gga Gly g cg Ala cat His 180 gat Asp gtc Val ctc Le u tct Ser aat Asn 260 g ca Ala cga Arg ttg Le u cct Pro aag Lys gag Giu 165 ca g Gin gac Asp att Ile ga t Asp gag Gi u 245 ga c Asp tgg Trp caa aga gag tac ggc gcc gga tta ccg tgaccagtgc Gin Arg Glu Tyr 285 Gly Ala Gly Leu Pro attgattggt gct <210> 670 <211> 290 <212> PRT <213> Corynebacterium glutamicum WO 01/00805 <400> 670 Met Ilie Tyr Ar 1 Ser Leu Gly Le 2 Gin Arg Ser Ii Asp Leu Ala As Phe Giy Arg Il Ile Ile Ser Se Phe Giy Giy Se Thr Arg Leu Gi 115 Asp Pro Asp Th 130 Val Ala Ser Gi 145 Giu Leu Thr Ai Leu Leu Ile Hi 18 Giu Pro Giy As 195 Gly Leu Giy Va 210 Asp Lys Tyr Le 225 Lys Ser Leu Se Arq Lys Leu As 26 PCTJIBOO/00926 g Arg 5 u Trp 0 e Ile Asn e Leu r Lys r Arq 0 y Leu r Pro y Lys a Giu 165 s Gin 0 p Asp 1Ile Asp r Giu 245 Asp 0 Val Giy Asn Ser Giy Leu Lys Leu Pro Ala Ile 10 His His Tyr Arg 70 Al a Lys Asp Le u Ala 150 Ala Pro Gly Ala Gly 230 Gi y Ile Asn Arg Gly 55 Giu Gly Tyr Tyr Giu 135 Le u Ala Ser Giu Phe 215 Ile Met Ala Phe Ala 40 Pro Asp T rp Leu Val1 120 Giu T yr Giu T yr Asn 200 Ser Pro Leu Gin Gi y 25 Phe Pro Leu Asp Vali 105 Asp Thr Vai Phe Ser 185 Leu Pro Giu Asn Giu 265 Asp Arg Giy Ser .7 5 Trp Se r Phe Tyr le 155 Ala Ile Gin Ala Ser 235 Asn Gly Lys Gly Se r His Pro Le u T yr Al a Ser Giu Asn Ser Gin 220 Arg Asn Gin Pro Val Aia Arg Gi y Asp His 125 Le u Ser Giu Arg Ala 205 Giy Aia Ile Ser Leu Thr Gi u Asp Pro Gin 110 His Arg Tyr Gly Trp 190 Ala Leu Ser Asp Leu 270 Thr Phe As n Le u Giy Le u Pro Ile Pro 160 Pro Gi u Asn Th r Gi y 240 Val Gin Met Ala Leu Ala Trp Val Leu Arg Glu Gin Arg Giu Gly Ala Giy 2 Leu Pro 290 <210> 671 280 WO 01/00805 <211> 1068 <212> DNA <213> Corynebacterium glutamicum <220> <221> COS <222> (101)..(1045) <223> RXN00024 <400> 671 ttaagacatc aacatatggc ttgtgctact gaaagatttt acgctcctat gctcggggca gtaagttgtg agcataggaa PCTIIBOO/00926 tcttctgaaa ttctgtagaa atg gag cac ggc gtg Met Giu His Gly Val acc Thr g ga Gly t tg Leu tct pr gag Glu gct Al a gca Ala t cg Ser gat Asp a ag Lys 150 ttt Phe cac gt t Val aat As n ga t Asp tat Tyr ctc Le u ac c Thr act Thr atc Ile gag Giu 135 cac His gag G lu tac att Ile acc Thr gca Ala tct Ser ggC Gi y aag Lys ttc Phe gat Asp 120 ca g Gin ctc Le u aag Lys aac aaa Lys ttt Phe t tc Phe gca Ala gcg Ala tct Ser aag L ys 105 att Ile gtt Val g ca Ala t ct Se r ctg ggc Gi y ggc Gi y gtg Val1 t gg Trp t gg Trp ggt Giy gct Aila ttt Phe gct Aila ttg Le u gta Val1 170 gt g act Thr t gg Trp gca Ala gt t Val1 att Ile 75 gcg Ala gjt c Val1 tac Tyr atc Ile tct Se r 155 ggc Gly t cg gaa Glu a cc Thr gcg Ala gaa Giu 60 aag Ly s ttg Le u gag Glu tac Tyr gct Al a 140 aac Asn act Thr agg ttt Ph e t cg Ser gga Gi y 45 gg c Gly gaa Glu gag Glu ggt Gly cac His 125 aat Asn tac Tyr cca Pro aag gat Asp aat As n 30 gga Gly aat Asn cg t Arg cct Pro t cc Ser 110 tac T yr gat Asp agc Se r gcg Al a ga t gtt Val1 15 agg Arg aac Asn gag Glu ggc Gi y gtt Val 95 ctg Leu gac Asp ctg Leu gcg Ala cag Gin 175 tat ttc Phe gaa Glu ttt Phe ggt Gly gca Ala 80 gc t Ala gag Giu gat Asp att Ile gag Glu 160 c cg Pro gag 1 cca Pro cag Gln gtt Val ggc Gly gac Asp ggt Gl y cgt Arg gag Glu g ca Ala 145.

cg t Arg gt t Val1 gag cta Le u acc Thr gac Asp gag Glu aag Lys cg t Arg ttg Le u gca Ala 130 cag Gin tta Leu gct Ala aac aac As n ttc Phe acc Thr t cg Ser ctg Leu tcc Se r gg9C Gly 115 gtc Val1 gg C Gly gc t Ala ctg Leu gtg ctc ggt Leu Gly gcg gtt Ala Val gcc gat Ala Asp gag cgg Glu Arg atc att Ile Ile cgt gag Arg Glu 100 gtg gaa Val Giu agc att Ser Ile aag att Lys Ile gag ttc Giu Phe 165 caa ccg Gin Pro 180 cag cca 115 163 211 259 307 355 403 451 499 547 595 643 691 His ctc Leu gcq Ala aaa Lys 230 9CC Ala cca Pro ccg Pro qtg Val WO 01/00805 Tyr ASfl Leu 185 gcc gag aag Ala Giu Lys 200 ggt ctt ttg Gly Leu Leu 215 gcg Cgt gcg Ala Arg Ala gtg gtg aca Vai Vai Thr acg act gtg Thr Thr Val 265 att gcg tcc Ilie Ala Ser 280 aag gat gtg Lys Asp Val 295 Val1 cat His acc Thr 999 Gi y gag Gl u 250 gcg Ala gt g Val gag Giu Ser Arg ggc gtt Giy Val gga aag Gly Lys 220 cag ttg Gin Leu 235 ttg cgt Leu Arg ctt gcg Leu Ala tcc aag Ser Lys ctg agc Leu Ser 300 Lys 9 ca Aila 205 tac Tyr ga t Asp 9 Ct Aila tgg T rp gta Val1 285 gct Ala Asp Tyr Giu 190 gtc ttc cct Val Phe Pro acc tcc aag Thr Ser Lys cgt tac 9CC Arg Tyr Ala 240 gtt 9cc gat Val Ala Asp 255 ttg gtt gcg Leu Val Ala 270 gag cag ttg Glu Gin Leu gag cag ctt Giu Gln Leu Glu tat T yr gag Glu 225 agc Ser gag Giu cat His aag Lys gca Ala 305 Asn Val 195 ttc gcg Phe Ala 210 gat att Asp Ile gat gag Asp Giu t tg ggt Leu Giy ggt gtg Gly Vai 275 gat ttg Asp Leu 290 cgt ttg Arg Leu Gin ctt Leu t cg Ser 9 C9 Ala gtt Val1 260 acc Thr atg Met gat Asp PCT1BOO/00926 Pro 9CC 739 Al a ggt 787 Gly ttt 835 Phe 245 gcg 883 Al a gca 931 Ala gct 979 Al a aag 1027 Lys gtt tcg gag cct ttc gct taagctctcc tcaaaagtaa gtg 1068 Vai Ser Giu Pro Phe Ala 310 315 <210> 672 <211> 315 <212> PRT <213> Corynebacteriun glutamicum <400> 672 Met Giu His Gly Val Thr Val Ile Lys 1 5 Pro Leu Asn Leu Gly Gly Asn Thr Phe 25 Gin Thr Phe Ala Val Leu Asp Ala Phe 40 Val Asp Thr Ala Asp Ser Tyr Ser Ala Gly Glu Ser Glu Arg Glu Leu Gly Ala 70 Asp Lys Leu Ile Ile Ala Thr Lys Ser Gly Arg Ser Arg Glu Ala Thr Phe Lys Gi y 10 Gly Val Trp Trp Gly 90 Al a Thr Trp Ala Val1 Ile Al a Val1 Phe Se r Gly Gly Glu Giu Gly Asp As n Gly Asn Arg Pro Se r Val1 Arg Asn GI u Gly Val1 Le u WO 01/00805 WO 0100805PCT[IBOO/00926 100 105 110 Arg Leu Gly Val Giu Ser Ile Asp Ile Phe Tyr Tyr His Tyr Asp Asp 115 120 125 Glu Ala Val Ser Ile Asp Giu Gin Vai Ala Ile Ala Asn Asp Leu Ile 130 135 140 Ala Gin Giy Lys Ile Lys His Leu Ala Leu Ser Asn Tyr Ser Ala Giu 145 150 155 160 Arg Leu Ala Giu Phe Phe Giu Lys Ser Val Giy Thr Pro Ala Gin Pro 165 170 175 Vai Ala Leu Gin Pro His Tyr Asn Leu Vai Ser Arg Lys Asp Tyr Giu 180 185 190 Giu Asn Vai Gin Pro Leu Ala Giu Lys His Giy Vai Ala Vai Phe Pro 195 200 205 Tyr Phe Ala Leu Ala Ala Giy Leu Leu Thr Gly Lys Tyr Thr Ser Lys 210 215 220 Giu Asp Ile Ser Gly Lys Ala Arg Ala Gly Gin Leu Asp Arg Tyr Ala 225 230 235 240 Ser Asp Giu Ala Phe Ala Val Val Thr Giu Leu Arq Aia Val Ala Asp 245 250 255 Glu Leu Giy Val Ala Pro Thr Thr Val Ala Leu Ala Trp Leu Vai Ala 260 265 270 His Gly Vai Thr Ala Pro Ile Ala Ser Val Ser Lys Vai Giu Gin Leu 275 280 285 Lys Asp Leu Met Ala Val Lys Asp Val Giu Leu Ser Ala Giu Gin Leu 290 295 300 Ala Arg Leu Asp Lys Val Ser Giu Pro Phe Ala 305 310 315 <210> 673 <211> 1758 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1735) <223> RXN01164 <400> 673 gccgatcgtg attgatqaag acgagatcca agcctggact tctgatctca aacctgaaga tttcaccaaa ggtaaagatg aatccqacgg tgaqaaataa gtq aca ctg ttt gtt 115 Val Thr Leu Phe Val 1 cgg ctc qcc ctt gct gct qtq gqc gqg ctt ttt gtc ttt qct tcc aat 163 Arg Leu Ala Leu Ala Ala Val Gly Gly Leu Phe Val Phe Aia Ser Asn WO 01/00805 WO 0100805PCT/TB00100926 ga a Glu ttt Ph e aag Lys act Th r tat Tyr tat Tyr gct Ala ttt Phe ttt Phe 150 ccg Pro tcc Ser aa g Lys gcg Al a atc Ile 230 ttc P he ccg Pro atc Ile a ag Lys gtt ValI t tg Leu gtC ValI ttc Phe c cg Pro 135 gat Asp ttg Leu acg Thr cga Arg gtg Val 215 gaa Glu aat Asn ggc Gi y ctt Le u gag GI u cag Gin ctg Leu ttg Le u 105 gtg Val1 a tg Met ttc Phe aat Asn ctg Leu 185 gcc Ala t cc Se r gcc Ala caq Gin t gg Trp g cg Al a cca Pro* ggC Gly ttg Leu tca Ser ctc Le u tat T yr gcq Al a ctc Le u 170 gct Ala ggc Gly ctg Leu gca Al a cgc Arg 250 ttt Phe ccg Pro gt c ValI atq Met 75 ccg Pro gtt Val att Ile gtg Val tg Trp 155 gca Al a qcc Ala gc a Ala tac Tyr att Ile 235 cgc Arg gtc Val1 tgg T rp cca Pro 60 ctt Leu t gg Trp gtc Val1 g cg Ala g Ct Al a 140 gtt ValI g cg Ala gtg Val atc Ile gt t Val1 220 ca g Gin 9q Al a gcg gqa Ala Gly 30 gat ctg Asp Leu ttt ttg Phe Leu tta qqt Leu Gly atc ggt Ile Gly gag gcq Giu Ala 110 cgt tgg Arg Trp 125 qtq gag Val Giu cgc ctg Arg Leu ctt qgt Leu Gly ggt gtg Gly Val 190 atc acc Ile Thr 205 gac cgc Asp Arg ggc aat Gly Asn gtg ctq Val Leu 15 att Ile g ga Gly caa Gin ttt Phe gag Giu 95 ctt Leu agg Arg tat T yr gca Al a qgg Gly gcc Ala qcg Al a aat As n gtg Val q cg Ala 255 gtt Val1 gtt Val cag Gin gtc ValI 80 ttt Phe tat Tyr gac Asp cta Le u tgg T rp 160 gt a Val atg Met aqt Ser gg C Gly cct Pro 240 aat As n gg9c Gly ccc Pro atg Met cat His qt t Val1 t CC Se r tgg Trp aga Arg 145 ggt Gi y gcg Ala gtg Val qtg Val a cg Thr 225 cgg Arg cac His act Th r caa Gin tcc Ser 9 gC Gly ggc Gly at t Ile a ag Ly s 130 agc Se r caa Gin ttt Phe att Ile att Ile 210 ag C Se r atg Met gca Ala gca Al a aag Lys a cq Thr ct g Le u tca Ser gct Ala 115 qtt ValI t cg Se r att Ile gtc Val att Ile 195 gc t Ala gat Asp gga Gly c qg Arg tta Leu cg9 Arg ggc Gi y gtg Val1 ctg Leu 100 ct t Leu ct C Leu tg T rp aac Asn act Thr 180 t CC Se r atc Ile gaa Gi u ttg Leu gaa Glu 260 ttt Phe cg g Arg cca Pro aca Th r cct Pro gg t Gi y ct g Le u cca Pro gg t Gly 165 tt t Phe aag Lys gqc Gly agc Ser gac Asp 245 acc Th r 211 259 307 355 403 451 499 547 595 643 691 739 787 835 883 WO 01/00805 WO 0100805PCTIBOOIOO926 ctc Le u tca Se r gqa Gly qtC Val1 310 gaa G iu gaa Glu ga t Asp atg Met tgt Cys 390 ggg Gly acg Thr gaa Glu atc Ile gcc Ala 470 aag Lys qac Asp qca Al a 295 ga t Asp gg t Gly tac Tyr t cc Se r aat Asn 375 tac Tyr gc t Al a gac Asp ttt Phe gtc Val 455 qcc Ala ctg Leu qt C Val1 280 qtg Val gag Glu gcc Ala atg Met gct Ala 360 qc t Al a gag G lu gaa Glu atg Met ga t Asp 440 aac Asn acc Th r qa t Asp 265 a ac Asn qa a Giu gtt ValI g cg Al a ccg Pro 345 gga Gly gcg Ala gt C Val1 ttt Phe acg Th r 425 ag g Arg cct Pro t tq Leu ga a Gl u cca Pro cat His gq t Gi y gag Gi u 330 ttt Phe aac Asn aac As n atc Ile ttg Le u 410 tat Tyr g cg Al a gat Asp a cg Thr caa Gin ttt Phe gt t Val cca Pro 315 tac Tyr cgc Ar g ttc Phe tta Le u ttc Phe 395 acc Thr c ag Gin gt g ValI gg a Gi y ga a Glu 475 gtg Val1 tcc Ser c ag Gin 300 cgc Arg cac His gaa Gi u cag Gin Gly 380 gac Asp acq Thr caa Gin gtt Val agc Se r 460 tcc Se r ga t Asp ga t Asp 285 qca Al a aac Asn aat Asn ttc Phe

CCC

Pro 365 cgc Arg cg t Arg

CCC

Pro tta Leu gtt Val 445 att Ile att Ile t tg Le u 270 qca Ala Cct Pro acc Thr aag Lys Ct g Le u 350 gqt Giy gct Al a gc t Aila acc Thr gca Al a 430 gca Al a tcc Ser cca Pro gtg Vali caa Gin att Ile atg Met aag Lys 335 aqa Arg ga t Asp gtg Val1 gq C Giy aac Asn 415 atg Met gct Ala caa Gin ctc Le u atC Ilie gc a Aia ttq Le u cag Gln 320 ttC Ph e att Ilie Gly aca Thr cgc Arg 400 aac Asn agc Ser aca Th r aac Asn aag Lys 480 ct g Le u t gg Trp aga Arg gq Val 305 gt a Val1 ttg Le u ttc Phe acc Thr qtg Val1 385 qac Asp gcc Aila a gg Arg t cg Se r acc Thr 465 qac Asp ttg Le u ccq Pro gcc Aia 290 qgc Giy ttt Ph e cag Gin t cg Se r ggc Gly 370 gqc Gly gcc Ala acc Th r atg Met ggt Giy 450 cga Arg act Th r gtt ValI gag Giu 275 att Ilie a cg Thr gat Asp ccg Pro ccc Pro 355 gta Val1 gtg ValI atc Ile ttc Ph e cgt Arg 435 gtt Val1 att Ile gt c Val1 atc Ile aat As n atc Ile atc Ile cct Pro ttt Phe 340 tac T yr gtg Val1 atg Met gcc Al a ga Gly 420 gc c Al a t cg Ser t tt Phe acc Thr att Ile 500 tcc Se r g at Asp acc Thr gtt Vali 325 gg t Gly gt t Val1 gag Glu a cq Thr aat Asn 405 ttc Phe atc Ile gc t Ala gag Glu atc Ile 485 ggt Giy 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 qca qcg cgg gtt ggt ttc tat gtt gaa tta Aia Ala Arg Val Phe Tyr Val Glu Leu 495 WO 01/00805 PCTJIBOO/00926 qta tta gjct gga cta ttc qcc att cqa atg aat aqc cgt tca aaq tct 1651 Val Leu Ala Gly Leu Phe Ala Ile Arg Met Asn Ser Arq Ser Lys Ser 505 510 515 gcg aaa ggt tcc gct cgg ccc qca caa gtt cqg gtt aag aag gtg cct 1699 Ala Lys Gly Ser Ala Arg Pro Ala Gin Val Arg Val Lys Lys Val Pro 520 525 530 qcq aaa aag gca qca act aat cgt cga aaa gta aaa taaaaacgtc 1745 Ala Lys Lys Ala Ala Thr Asn Arg Arg Lys Val Lys 535 540 545 ccgaagggac gag 1758 <210> 674 <211> 545 <212> PRT <213> Corynebacterium giutamicum <400> 674 Val Thr Leu Phe Val Arg Leu Ala Leu 1 Val1 Gly Pro Met His Val Ser Trp Arg 145 Gly Ala Val1 Val1 Phe Thr Gin Ser Gly Gi y Ile Lys 130 Se r Gin Phe Ile Ile Ala Ala L ys Thr Leu Ser Ala 115 Val1 Ser Ile Val Ilie 195 Ala Se r Le u Arg Gly Val1 Le u 100 Leu Le u T rp As n Thr 180 Se r Ile 5 Asn Phe Arg Pro Thr Pro Gly Leu Pro Gly 165 Phe Lys Gly Giu Phe Lys Thr 70 T yr T yr Ala Phe Phe 150 Pro Ser Ly s Ala Pro Ile Lys 55 Val1 Le u Val1 Phe Pro 135 Asp Le u Thr Arg Val1 Ile Ser 40 As n Val1 Gin Ala Gly 120 Ala Gly Ala Val Le u 200 Ser Gly 25 Le u Giu Gin Leu Le u 105 ValI Met Phe Asn Le u 185 Ala Ser Ala 10 Trp Al a Pro Gly Leu 90 Ser Le u Tyr Ala Leu 170 Ala Gly Leu Ala Phe Pro Val1 Met 75 Pro Val1 Ile Val1 T rp 155 Ala Al a Ala Tyr Val1 Val1 Trp Pro Leu T rp Val1 Al a Ala 140 Val1 Ala Val1 Ile Val Gly Ala Asp Phe Leu Ile Glu Arg 125 Val1 Arq Leu Gly Ile 205 Asp Gly Gly Leu Leu Gly Gly Ala 110 T rp Glu Leu Gly Val 190 Th r Arg Leu Ile Gly Gin Phe Glu Leu Arg Tyr Ala Gly 175 Al a Ala As n Ph e ValI Val Gin Val1 Phe T yr Asp Leu T rp 160 Val Met Ser Gly WO 01/00805 PCT/IB00/00926 210 215 220 Thr Ser Asp Glu Ser Ile Glu Val Ala Ala Ile Gin Gly Asn Val Pro 225 230 235 240 Arg Met Gly Leu Asp Phe Asn Ala Gin Arg Arg Ala Val Leu Ala Asn 245 250 255 His Ala Arg Glu Thr Leu Lys Leu Asp Glu Gin Val Asp Leu Val Ile 260 265 270 Trp Pro Glu Asn Ser Ser Asp Val Asn Pro Phe Ser Asp Ala Gin Ala 275 280 285 Arg Ala Ile Ile Asp Gly Ala Val Glu His Val Gin Ala Pro Ile Leu 290 295 300 Val Gly Thr Ile Thr Val Asp Glu Val Gly Pro Arg Asn Thr Met Gin 305 310 315 320 Val Phe Asp Pro Val Glu Gly Ala Ala Glu Tyr His Asn Lys Lys Phe 325 330 335 Leu Gin Pro Phe Gly Glu Tyr Met Pro Phe Arg Glu Phe Leu Arg Ile 340 345 350 Phe Ser Pro Tyr Val Asp Ser Ala Gly Asn Phe Gin Pro Gly Asp Gly 355 360 365 Thr Gly Val Val Glu Met Asn Ala Ala Asn Leu Gly Arg Ala Val Thr 370 375 380 Val Gly Val Met Thr Cys Tyr Glu Val Ile Phe Asp Arg Ala Gly Arg 385 390 395 400 Asp Ala Ile Ala Asn Gly Ala Glu Phe Leu Thr Thr Pro Thr Asn Asn 405 410 415 Ala Thr Phe Gly Phe Thr Asp Met Thr Tyr Gln Gin Leu Ala Met Ser 420 425 430 Arg Met Arg Ala Ile Glu Phe Asp Arg Ala Val Val Val Ala Ala Thr 435 440 445 Ser Gly Val Ser Ala Ile Val Asn Pro Asp Gly Ser Ile Ser Gin Asn 450 455 460 Thr Arg Ile Phe Glu Ala Ala Thr Leu Thr Glu Ser Ile Pro Leu Lys 465 470 475 480 Asp Thr Val Thr Ile Ala Ala Arg Val Gly Phe Tyr Val Glu Leu Leu 485 490 495 Leu Val Ile Ile Gly Val Leu Ala Gly Leu Phe Ala Ile Arg Met Asn 500 505 510 Ser Arg Ser Lys Ser Ala Lys Gly Ser Ala Arg Pro Ala Gin Val Arg 515 520 525 Val Lys Lys Val Pro Ala Lys Lys Ala Ala Thr Asn Arg Arg Lys Val 530 535 540 wo 01/00805 WO 0100805PCTIBOOIOO926 Lys 545 <210> 675 <211> 933 <212> DNA (213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(910) <223> RXN01168 <400> 675 ccgcacaaqt tcgggttaaq aaggtgcctg cgaaaaaggc agcaactaat cgtcgaaaag taaaataaaa acgtcccgaa gggacgagga ggacaacacc atg agc agt Met Ser Ser gag gca Glu Ala gta Val1 aac Asn cac His gca Ala gaa Glu ggc Gly tcc Ser 9gc Gi y gtc ValI ga t Asp ctt Leu gta Val gac Asp ggc Gi y ctg Le u cac His gct Ala aac Asn 135 gct Al a cca Pro ctc Leu aag Lys aaa Lys gag Glu gca Ala gac Asp 120 tgg T rp acg Th r ctc Le u atc Ile ctt Le u qg c GI y cg c Arq cca Pro 105 ctg Leu

CCC

Pro qt g ValI ga t Asp gac Asp ga c Asp 60 tqc Cys cag Gin ctg Le u Gly cg t Arg 140 at t Ile cqc Arq aac Asn 45 ga c Asp gca Ala gt c Val cac His tcg Se r 125 t 99 Trp att Ilie gtg Val1 30 ag c Ser qa c Asp gag Glu ctg Le u ctg Le u 110 cq C Arg ctc Leu cca Pro Cq C Arg cca Pro ca c His tac Tyr tgc Cys Ctg Leu tac Tyr ttg Le u ttq a cg Thr a cc Thr ga C Asp att Ile atg Met 80 gaa Glu ctc Leu gtg Val1 tCC Ser acc Th r 160 1 tac Tyr qca Ala 99 C Gly ttt Ph e gC t Ala atq Met gct Al a cca Pro aag Lys 145 gat Asp aac Asn acc Thr acc Th r gt C Val1 Gly ga c Asp gag Glu 99 C Gly 130 99 C Gly atq Met gag Glu cct Pro 99C Gly ctc Leu ttc Phe 9CC Ala atc Ile 115 gqC Gly 99 C Gl y acc Thr Ctg Leu qa c Asp gag Glu cac His cag Gln gac Asp 100 acc Th r Cg C Arg aac As n gca Al a gaa Glu gtt Val1 CgC Arg Cg C Arq t 99 Trp ggC Gly aat As n gta Val1 gt C ValI 999 Gly 165 115 163 211 259 307 355 403 451 499 547 595 tac atc agc qtc gcg ctc 99C gCc ggc Tyr Ile Ser Val Ala Leu Gly Ala Gly Leu WO 01/00805 tac cgc gct ttt cga cgt gaa gtg Tyr Arg Ala Phe Arg Arg Glu Val I 170 ctc tcc aac gct ggg tac att ttc c Leu Ser Asn Ala Gly Tyr Ile Phe C 185 gtt gaa gcc gga ttc gat gtt cgt c Vai Giu Ala Gly Phe Asp Val ArgC 200 205 cgt gag atc ggc gaa tcc aag ctg Arg Glu Ile Gly Giu Ser Lys Leu i 215 220 ctg ctc gag gta acc aag tgg ggc c Leu Leu Giu Val Thr Lys Trp Giy 1 230 235 aag gaa ctg tcc aag gaa atg gtc Lys Giu Leu Ser Lys Giu Met Val 250 cac ttc aaa aag cgc aac acc tgg His Phe Lys Lys Arg Asn Thr Trp 265 qaa <210> 676 <211> 270 <212> PRT <213> Corynebacteriui glutamicum <400> 676 Met Ser Ser Glu Ala Vai Asp Ala 1 5 Tyr Asn Glu Leu Glu Asn Leu Pro Ala Thr Pro Asp Val His Val Leu Gly Thr Giy Glu Arg Ala Asp Lys 55 Phe Val Leu His Arg Glu Gly Lys 70 Ala Gly Phe Gin Trp Giy Leu Giu Met Asp Ala Asp Giy Ser His Ala 100 Ala Glu Ilie Thr Asn Gly Ala Asp 115 120 PCTIIBOO/00926 lcq 643 gaa gca ctg ccg ctt gat Glu Ala Leu Pro Leu Asp 175 180 gtt gag att gcc tac cgt Val Giu Ile Ala Tyr Arg 195 gtt ccc atc act ttc acc Val Pro Ile Thr Phe Thr 210 ggc agc ttt gtc aag gat Giy Ser Phe Vai Lys Asp 225 aag cac cgc ggt ggc cag Lys His Arg Gly Gly Gin 240 ctg ctq aac tat gag tgg Leu Leu Asn Tyr Giu Trp 255 260 taaactgctt gccggttagt Leu Val1 Asp Ala Leu Tyr Gin Ile Val Asp Asp Asp C ys Gin Leu Gly Pro Arg Pro His Tyr Cys Leu Tyr P rc Lys 145 Asr LeL I lE Ilc PhE 22' Ar Asi WO 01/00805 Gly Gly A 130 Gly Gly A Met Thr A Pro Leu A 1 Ala Tyr A 195 Thr Phe T 210 SVal Lys A 1 Gly Gly G nl Tyr Giu T 2 sn la sp 80 rg hr sp in rp 60 Val Val1 Gi y 165 Glu Ala Glu Ser Ala 245 Lys Va 1 T yr 150 Tyr Le u Val1 Arg Le u 230 Ly s His As n 135 Ile Arg Ser Glu Giu 215 Leu Giu Phe Pro Val Phe Ala 185 Gly Gly Val1 Ser Lys 265 Lys Al a Arg 170 Gly Phe Glu Thr Lys 250 A r c Asn Leu 155 Arg Ty r Asp Ser Lys 235 Glu Asn Arq 140 Gly Glu Ile Val Lys 220 Trp Met Thr T rp Ala Val1 Ph e Arg 205 Leu Gly Val1 Trp Le u Gly Le u Gin 190 Giu Asp Leu Gly Leu 270 PCTABOO/00926 Ser Thr 160 Ala Glu Pro Ser His 240 Leu BGI-125CPPC 47

Claims (27)

1. An isolated Corynebacterium glutarnicum nucleic acid molecule comprising the nucleotlde sequence set forth in SEQ ID NO:5, or a complement thereof.

2. An isolated nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:6, or a complement thereof.

3. An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:6, or a complement thereof.

4. An isolated nucleic acid molecule comprising a nucleotide sequence which is functionally equivalent, and at least 50% identical, to the entire nucleotide sequence set forth in SEQ ID NO:5, or a complement thereof.

5. An isolated nucleic acid molecule comprising a fragment of at least contiguous nucleotides of the nucleotide sequence set forth in SEQ ID or a complement thereof. 15 6. An isolated nucleic acid molecule comprising the nucleic acid molecule of any one of claims and a nucleotide sequence encoding a heterologous polypeptide. o

7. A vector comprising the nucleic acid molecule of any one of claims 1 to 6.

8. The vector of claim 7, which is an expression vector.

9. A host cell transfected with the expression vector of claim 8. host cell of claim 9, wherein said cell is a microorganism.

11.The host cell of claim 10, wherein said cell belongs to the genus Corynebacterium or Brevibacterum. COMS ID No: SBMI-01710428 Received by IP Australia: Time 15:03 Date 2005-10-11 11/10 '05 TUE 14:56 FAX 61 2 9888 7600 WATERMARK l007 124

12.The host cell of claim 9, wherein the expression of said nucleic acid molecule results in the modulation in production of a fine chemical from said cell.

13.The host cell of claim 12, wherein said fine chemical is selected from the group consisting of: organic acids, nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides, and enzymes.

14.A method of producing a polypeptide comprising culturing the host cell transfected with the vector of claim 8 in an appropriate culture medium to, thereby, produce the polypeptide.

15.A polypeptide produced by the method of claim 14.

16.The polypeptide of claim 15, wherein said polypeptide is involved in the production of a fine chemical production. 15

17.An isolated polypeptide comprising an amino acid sequence set forth in SEQ ID NO:6.

18.An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:6. 9

19.An isolated polypeptide which is encoded by a nucleic acid molecule 20 comprising a nucleotide sequence which is functionally equivalent, and at least identical, to the entire nucleic acid sequence set forth in SEQ ID isolated polypeptide comprising an amino acid sequence which is functionally equivalent, and at least 50% identical, to the entire amino acid sequence set forth in SEQ ID NO:6. COMS ID No: SBMI-01710428 Received by IP Australia: Time 15:03 Date 2005-10-11 11/10 '05 TUTE 14:57 FAX 61 2 9888 7600WAEAR d08 WATERMARK Im 008 125 21 .An isolated polypeptide comprising a fragment of the amino acid sequence set forth in SEQ ID NO:6, wherein said fragment maintains an ammonium transport system activity.

22.The isolated polypeptide of any one of claims 15 to 21 further comprising heterologous amino acid sequences.

23.A method for producing a fine chemical, comprising culturing a host cell transfected with the vector of claim 8 such that the fine chemical is produced.

24. The method of claim 23, wherein said method further comprises the step of recovering the fine chemical from said culture.

25.The method of claim 23, wherein said cell belongs to the genus Coiynebac'erium or erevibacterium

26. The method of claim. 23, wherein said cell is selected from the group consisting of: Cotynebacteriur glutamicum, Colynebacterium herculis, Corynebacteriurn, lflium, Cofynebacterium acetoacidophilum, Corynebacterirn is1 acetoglutamicum, Corynebacterium acetophilum, Coiynebactenium ammonia genes, Corynebacterium fujiokense, Corynebacterium nit ri/aphi/us, 9 9Brevibactenium ammoniagenes, Brevibacternum butanicum, Brevibacterium divanrcatum, Brevibacterium f/a vum, Brevibacterium heali, Brevibacterium ketoglutamicum, Brevibacterium ketosoreductum, Brevibacterium 999920 lactoferrnenwum, Breibacterium linens, Brevibactetium paraffinolyticum, and .9.9.9those strains set forth in Table 3.

27. The method of claim 23, wherein expression of the nucleic acid molecule from said vector results In modulation of production of said fine chemical.

28. The method of claim 23, wherein said fine chemical is selected from the group consisting of: organic acids, nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated COMS ID No: SBMI-01710428 Received by IP Australia: Time 15:03 Date 2005-10-11 11/10 '05 TUE 14:57 FAX 61 2 9888 7600WAEAR 09 WATERMARK Q 009 126 fatly acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, Polyketides, and enzymes.

29. The method of claim 23, wherein said fine chemical is an amino acid. method of claim 29, wherein said amino acid is drawn from the group consisting of: lysine, glutamate, glutamine, alanine, aspartate, glycine, serine, threonine, methionine, cysteine, valine, leucIne, isoleucine, arginine, proline, histidine, tyrosine, phenylalanins, and tryptophan. 31 .A method for producing a fine chemical, comprising culturing a cell whose genomic DNA has been altered by the inclusion of the nucleic acid molecule of any one of claims 1 to 6. S 032.A method for diagnosing the presence or activity of Coiynebactetiuum diphtheriae In a subject, comprising detecting the presence of at least one of S the nucleic acid molecules of claims 1 to 6 or at least one of the Polypeptide molecules of claims 17 to 22, thereby diagnosing the presence or activty of 15 Colynebacterium diphtheniae in the subject. 1.

33.A host cell comprising a nucleic acid molecule comprising the nucleotide .sequence set forth in SEQ ID NO:5, wherein the nuclelc acid molecule is disrupted. to l:3.'o t c ll c m rs n u l ic a i o e u e c m ri i g t e n ce td 2 0s q e c e t f r h i E Q I 5 h r i n t e n c e c c d m l c l 4 6 0 c o p i e nSr m r u l i c d m d f c t o s a o p r d t h seuec se.ot nS DN:5 COMS ID No: SBMI-01710428 Received by IP Australia: Time 15:03 Date 2005-10-11 11/10 '05 TUE 14:57 FAX 61 2 9888 7600 WATERMARK Q010i 127 host cell Comprising a nucleic acid molecule Comprising the niucleotide sequence set forth In SEQ ID NO:5, wherein the regulatory region of the nucleic acid molecule is modified relative to the wild-type regulatory region of the molecule.

36. A fine chemical produced by the method of any one of claims 23 to 31. DATEDthis 4th day of October 2005 BASF AKTIENGESELLSCHAFT. WATERMARK PATENT TRADE MARK ATTORNEYS 290 BUR WOOD ROAD HAWTHORN VICTORIA 3122 AUSTRALIA P20678AUOO COMS ID No: SBMI-01710428 Received by IP Australia: Time 15:03 Date 2005-10-11