AU2006200802A1 - Cornebacterium Glutamicum genes encoding proteins involved in homeostasis and adaption - Google Patents

Description

P001 Section 29 Regulation 3.2(2)

AUSTRALIA

Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT Application Number: Lodged: Invention Title: Cornebacterium Glutamicum genes encoding proteins involved in homeostasis and adaption The following statement is a full description of this invention, including the best method of performing it known to us: N CORYNEBACTERIUM GLUTAMICUM GENES ENCODING PROTEINS SINVOLVED IN HOMEOSTASIS AND ADAPTATION Related Applications This application claims priority to prior filed U.S. Provisional Patent Application Serial No. 60/141031, filed June 25, 1999. This application also claims priority to prior 00 filed German Patent Application No. 19931636.8, filed July 8, 1999, German Patent Application No. 19932125.6, filed July 9, 1999, German Patent Application No.

S19932126.4, filed July 9, 1999, German Patent Application No. 19932127.2, filed July NI 10 9, 1999, German Patent Application No. 19932128.0, filed July 9, 1999, German Patent Application No. 19932129.9, filed July 9, 1999, German Patent Application No.

19932226.0, filed July 9, 1999, German Patent Application No. 19932920.6, filed July 14, 1999, German Patent Application No. 19932922.2, filed July 14, 1999, German Patent Application No. 19932924.9, filed July 14, 1999, German Patent Application No.

19932928.1, filed July 14, 1999, German Patent Application No. 19932930.3, filed July 14, 1999, German Patent Application No. 19932933.8, filed July 14, 1999, German Patent Application No. 19932935.4, filed July 14, 1999, German Patent Application No.

19932973.7, filed July 14, 1999, German Patent Application No. 19933002.6, filed July 14, 1999, German Patent Application No. 19933003.4, filed July 14, 1999, German Patent Application No. 19933005.0, filed July 14, 1999, German Patent Application No.

19933006.9, filed July 14, 1999, German Patent Application No. 19941378.9, filed August 31, 1999, German Patent Application No. 19941379.7, filed August 31, 1999, German Patent Application No. 19941390.8, filed August 31, 1999, German Patent Application No. 19941391.6, filed August 31, 1999, and German Patent Application No.

19942088.2, filed September 3, 1999. The entire contents of all of the aforementioned applications are hereby expressly incorporated herein by this reference.

Background of the Invention Certain products and by-products of naturally-occurring metabolic processes in cells have utility in a wide array of industries, including the food, feed, cosmetics, and pharmaceutical industries. These molecules, collectively termed 'fine chemicals', include organic acids, both proteinogenic and non-proteinogenic amino acids, -2r, nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, and enzymes. Their production is most conveniently performed through the large-scale culture of bacteria developed to produce

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and secrete large quantities of one or more desired molecules. One particularly useful organism for this purpose is Corynebacterium glutamicum, a gram positive, O nonpathogenic bacterium. Through strain selection, a number of mutant strains have

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O been developed which produce an array of desirable compounds. However, selection of N strains improved for the production of a particular molecule is a time-consuming and C difficult process.

(N Summary of the Invention The invention provides novel bacterial nucleic acid molecules which have a variety of uses. These uses include the identification of microorganisms which can be used to produce fine chemicals, the modulation of fine chemical production in C glutamicum or related bacteria, the typing or identification of C. glutamicum or related bacteria, as reference points for mapping the C. glutamicum genome, and as markers for transformation. These novel nucleic acid molecules encode proteins, ieferred to herein as homeostasis and adaptation (HA) proteins.

C. glutamicum is a gram positive, aerobic bacterium which is commonly used in industry for the large-scale production of a variety of fine chemicals, and also for the degradation of hydrocarbons (such as in petroleum spills) and for the oxidation of terpenoids. The HA nucleic acid molecules of the invention, therefore, can be used to identify microorganisms which can be used to produce fine chemicals, by fermentation processes. Modulation of the expression of the HA nucleic acids of the invention, or modification of the sequence of the HA nucleic acid molecules of the invention, can be used to modulate the production of one or more fine chemicals from a microorganism to improve the yield or production of one or more fine chemicals from a Corynebacterium or Brevibacterium species).

The HA nucleic acids of the invention may also be used to identify an organism as being Corynebacterium glutamicum or a close relative thereof, or to identify the presence of C. glutamicum or a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of C.

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N glutamicum genes; by probing the extracted genomic DNA of a culture of a unique or Smixed population of microorganisms under stringent conditions with a probe spanning a region of a C. glutamicum gene which is unique to this organism, one can ascertain C whether this organism is present. Although Corynebacterium glutamicum itself is nonpathogenic, it is related to species pathogenic in humans, such as Corynebacterium 0 diphtheriae (the causative agent of diphtheria); the detection of such organisms is of 00 0significant clinical relevance.

C, The HA nucleic acid molecules of the invention may also serve as reference \0 0points for mapping of the C. glutamicum genome, or of genomes of related organisms.

C 10 Similarly, these molecules, or variants or portions thereof, may serve as markers for genetically engineered Corynebacterium or Brevibacterium species.

The HA proteins encoded by the novel nucleic acid molecules of the invention are capable of, for example, performing a function involved in the maintenance of homeostasis in C. glutamicum, or in the ability of this microorganism to adapt to different environmental conditions. Given the availability of cloning vectors for use in Corynebacterium glutamicum, such as those disclosed in Sinskey et al., U.S. Patent No.

4,649,119, and techniques for genetic manipulation of C. gluramicum and the related Brevibacterium species lactofermentum) (Yoshihama et al, J. Bacteriol. 162: 591- 597 (1985); Katsumata et al., J. Bacteriol. 159: 306-311 (1984); and Santamaria et al., J.

Gen. Microbiol. 130: 2237-2246 (1984)), the nucleic acid molecules of the invention may be utilized in the genetic engineering of this organism to make it a better or more efficient producer of one or more fine chemicals. This improved production or efficiency of production of a fine chemical may be due to a direct effect of manipulation of a gene of the invention, or it may be due to an indirect effect of such manipulation.

There are a number of mechanisms by which the alteration of an HA protein of the invention may directly affect the yield, production, and/or efficiency of production of a fine chemical from a C. glutamicum strain incorporating such an altered protein. For example, by engineering enzymes which modify or degrade aromatic or aliphatic compounds such that these enzymes are increased or decreased in activity or number, it may be possible to modulate the production of one or more fine chemicals which are the modification or degradation products of these compounds. Similarly, enzymes involved in the metabolism of inorganic compounds provide key molecules phosphorous, -4sulfur, and nitrogen molecules) for the biosynthesis of such fine chemicals as amino Sacids, vitamins, and nucleic acids. By altering the activity or number of these enzymes in C. glutamicum, it may be possible to increase the conversion of these inorganic r compounds (or to use alternate inorganic compounds) to thus permit improved rates of incorporation of inorganic atoms into these fine chemicals. Genetic engineering of C.

glutamicum enzymes involved in general cellular processes may also directly improve 00 fine chemical production, since many of these enzymes directly modify fine chemicals amino acids) or the enzymes which are involved in fine chemical synthesis or Isecretion. Modulation of the activity or number of cellular proteases may also have a direct effect on fine chemical production, since many proteases may degrade fine chemicals or enzymes involved in fine chemical production or breakdown.

Further, the aforementioned enzymes which participate in aromatic/aliphatic compound modification or degradation, general biocatalysis, inorganic compound metabolism or proteolysis are each themselves fine chemicals, desirable for their activity in various in vitro industrial applications. By altering the number of copies of the gene for one or more of these enzymes in C. glutamicum it may be possible to increase the number of these proteins produced by the cell, thereby increasing the potential yield or efficiency of production of these proteins from large-scale C. glutamicum or related bacterial cultures.

The alteration of an HA protein of the invention may also indirectly affect the yield, production, and/or efficiency of production of a fine chemical from a C.

glutamicum strain incorporating such an altered protein. For example, by modulating the activity and/or number of those proteins involved in the construction or rearrangement of the cell wall, it may be possible to modify the structure of the cell wall itself such that the cell is able to better withstand the mechanical and other stresses present during large-scale fermentative culture. Also, large-scale growth of C.

glutamicum requires significant cell wall production. Modulation of the activity or number of cell wall biosynthetic or degradative enzymes may allow more rapid rates of cell wall biosynthesis, which in turn may permit increased growth rates of this microorganism in culture and thereby increase the number of cells producing the desired fine chemical.

By modifying the HA enzymes of the invention, one may also indirectly impact Sthe yield, production, or efficiency of production of one or more fine chemicals from C.

glutamicum. For example, many of the general enzymes in C. glutamicum may have a significant impact on global cellular processes regulatory processes) which in turn have a significant effect on fine chemical metabolism. Similarly, proteases, enzymes O which modify or degrade possibly toxic aromatic or aliphatic compounds, and enzymes 00 Swhich promote the metabolism of inorganic compounds all serve to increase the (N viability of C. glutamicum. The proteases aid in the selective removal of misfolded or Smisregulated proteins, such as those that might occur under the relatively stressful C 10 environmental conditions encountered during large-scale fermentor culture. By altering these proteins, it may be possible to further enhance this activity and to improve the viability of C. glutamicum in culture. The aromatic/aliphatic modification or degradation proteins not only serve to detoxify these waste compounds (which may be encountered as impurities in culture medium or as waste products from cells themselves), but also to permit the cells to utilize alternate carbon sources if the optimal carbon source is limiting in the culture. By increasing their number and/or activity, the survival of C. glutamicum cells in culture may be enhanced. The inorganic metabolism proteins of the invention supply the cell with inorganic molecules required for all protein and nucleotide (among others) synthesis, and thus are critical for the overall viability of the cell. An increase in the number of viable cells producing one or more desired fine chemicals in large-scale culture should result in a concomitant increase in the yield, production, and/or efficiency of production of the fine chemical in the culture.

The invention provides novel nucleic acid molecules which encode proteins, referred to herein as HA proteins, which are capable of, for example, performing a function involved in the maintenance of homeostasis in C. glutamicum, or of participating in the ability of this microorganism to adapt to different environmental conditions. Nucleic acid molecules encoding an HA protein are referred to herein as HA nucleic acid molecules. In a preferred embodiment, an HA protein participates in C.

glutamicum cell wall biosynthesis or rearrangements, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or possesses a C. glutamicum enzymatic or proteolytic activity. Examples of such proteins include those encoded by the genes set forth in Table 1.

The following embodiments, the subject of the invention of this application, Sare specifically disclosed herein: SAn isolated Corynebacterium glutamicum nucleic acid molecule Scomprising a nucleic acid sequence selected from the group C 5 consisting of each of the odd numbered SEQ ID NOs: 3 to 439, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement 00 oO thereof.

c• An isolated nucleic acid molecule which encodes a polypeptide comprising an amino acid sequence selected from the group c consisting of each of the even numbered SEQ ID NOs: 4 to 440, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 440, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

SAn isolated nucleic acid molecule comprising a nucleotide sequence which is at least 50% identical to the entire nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 439, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

An isolated nucleic acid molecule comprising a fragment of at least contiguous nucleotides of the nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 439, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.

An isolated polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered O SEQ ID NOs: 4 to 440, provided that the amino acid sequence is Snot encoded by any of the F-designated genes set forth in Table 1.

An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence l 5 selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 440, provided that the amino acid sequence is Snot encoded by any of the F-designated genes set forth in Table 1.

oo S* An isolated polypeptide which is encoded by a nucleic acid cN molecule comprising a nucleotide sequence which is at least 0 10 identical to the entire nucleic acid sequence of any of the odd cl numbered SEQ ID NOs: 3 to 439, provided that the nucleic acid molecule does not consist of any of the F-designated nucleic acid molecules set forth in Table 1.

An isolated polypeptide comprising an amino acid sequence which is at least 50% identical to the entire amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 440, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1.

An isolated polypeptide comprising a fragment of a polypeptide comprising the amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 440, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino sequence.

A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 439, wherein the nucleic acid molecule is disrupted.

A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 439, wherein the nucleic acid molecule comprises one or more nucleic acid modifications as compared to the sequence set forth in any of the odd-numbered SEQ ID NOs: 3 to 439.

IN* A host cell comprising a nucleic acid molecule selected from the Sgroup consisting of each of the odd numbered SEQ ID NOs: 3 to S439, wherein the regulatory region of the nucleic acid molecule is Smodified relative to the wild-type regulatory region of the molecule.

00 Accordingly, one aspect of the invention pertains to isolated nucleic acid molecules cDNAs, DNAs, or RNAs) comprising a nucleotide sequence encoding an HA protein or biologically active portions thereof, as well as nucleic acid fragments suitable as primers or hybridization probes for the detection or amplification of HAencoding nucleic acids DNA or mRNA). In particularly preferred embodiments, the isolated nucleic acid molecule comprises one of the nucleotide sequences set forth as the odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO: 1, SEQ ID C NO:3, SEQ ID NO:5, SEQ ID or the coding region or a complement thereof of one of these nucleotide sequences. In other particularly preferred embodiments, the C1 10 isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes to or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80% or 90%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence set forth as an odd-numbered SEQ ID NO in the Sequence Listing SEQ ID NO:1, SEQ ID NO:3, SEQ ID SEQ ID or a portion thereof. In other preferred embodiments, the isolated nucleic acid molecule encodes one of the amino acid sequences set forth in as an evennumbered SEQ ID NO in the Sequence Listing SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID The preferred HA proteins of the present invention also preferably possess at least one of the HA activities described herein.

In another embodiment, the isolated nucleic acid molecule encodes a protein or portion thereof wherein the protein or portion thereof includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence having an even-numbered SEQ ID NO: in the Sequence Listing), e.g., sufficiently homologous to an amino acid sequence of the invention such that the protein or portion thereof maintains an HA activity. Preferably, the protein or portion thereof encoded by the nucleic acid molecule maintains the ability to participate in the maintenance of homeostasis in C. glutamicum, or to perform a function involved in the adaptation of this microorganism to different environmental conditions. In one embodiment, the protein encoded by the nucleic acid molecule is at least about preferably at least about 60%, and more preferably at least about 70%, 80%, or 90% and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an amino acid sequence of the invention an entire amino acid sequence selected from C N those having an even-numbered SEQ ID NO in the Sequence Listing). In another preferred embodiment, the protein is a full length C. glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention (encoded by an open reading frame shown in the corresponding odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO:l, SEQ ID NO:3, SEQ ID NO:5, SEQ ID In another preferred embodiment, the isolated nucleic acid molecule is derived 00 from C. glutamicum and encodes a protein an HA fusion protein) which includes a Cr biologically active domain which is at least about 50% or more homologous to one of the amino acid sequences of the invention a sequence of one of the even-numbered Cr 10 SEQ ID NOs in the Sequence Listing) and is able to participate in the repair or recombination of DNA, in the transposition of genetic material, in gene expression the processes of transcription or translation), in protein folding, or in protein secretion in Corynebacterium glutamicum, or has one or more of the activities set forth in Table 1, and which also includes heterologous nucleic acid sequences encoding a heterologous polypeptide or regulatory regions.

In another embodiment, the isolated nucleic acid molecule is at least nucleotides in length and hybridizes under stringent conditions to a nucleic acid molecule comprising a nucleotide sequence of the invention a sequence of an oddnumbered SEQ ID NO in the Sequence Listing). Preferably, the isolated nucleic acid molecule corresponds to a naturally-occurring nucleic acid molecule. More preferably, the isolated nucleic acid encodes a naturally-occurring C. glutamicum HA protein, or a biologically active portion thereof.

Another aspect of the invention pertains to vectors, recombinant expression vectors, containing the nucleic acid molecules of the invention, and host cells into which such vectors have been introduced. In one embodiment, such a host cell is used to produce an HA protein by culturing the host cell in a suitable medium. The HA protein can be then isolated from the medium or the host cell.

Yet another aspect of the invention pertains to a genetically altered microorganism in which an HA gene has been introduced or altered. In one embodiment, the genome of the microorganism has been altered by introduction of a nucleic acid molecule of the invention encoding wild-type or mutated HA sequence as a transgene. In another embodiment, an endogenous HA gene within the genome of the -8- N, microorganism has been altered, functionally disrupted, by homologous Srecombination with an altered HA gene. In another embodiment, an endogenous or introduced HA gene in a microorganism has been altered by one or more point rC, mutations, deletions, or inversions, but still encodes a functional HA protein. In still another embodiment, one or more of the regulatory regions a promoter, repressor, or inducer) of an HA gene in a microorganism has been altered by deletion, 00 0truncation, inversion, or point mutation) such that the expression of the HA gene is modulated. In a preferred embodiment, the microorganism belongs to the genus Corynebacterium or Brevibacterium, with Corynebacterium glutamicum being particularly preferred. In a preferred embodiment, the microorganism is also utilized for the production of a desired compound, such as an amino acid, with lysine being particularly preferred.

In another aspect, the invention provides a method of identifying the presence or activity of Cornyebacterium diphtheriae in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention the sequences set forth in the Sequence Listing as SEQ ID NOs 1 through 440) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject.

Still another aspect of the invention pertains to an isolated HA protein or a portion, a biologically active portion, thereof. In a preferred embodiment, the isolated HA protein or portion thereof can participate in the maintenance of homeostasis in C. glutamicum, or can perform a function involved in the adaptation of this microorganism to different environmental conditions. In another preferred embodiment, the isolated HA protein or portion thereof is sufficiently homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: in the Sequence Listing) such that the protein or portion thereof maintains the ability to participate in the maintenance of homeostasis in C. glutamicum, or to perform a function involved in the adaptation of this microorganism to different environmental conditions.

The invention also provides an isolated preparation of an HA protein. In preferred embodiments, the HA protein comprises an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing).

In another preferred embodiment, the invention pertains to an isolated full length protein which is substantially homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) (encoded Sby an open reading frame set forth in a corresponding odd-numbered SEQ ID NO: of the Sequence Listing). In yet another embodiment, the protein is at least about I preferably at least about 60%, and more preferably at least about 70%, 80%, or and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an entire amino acid sequence of the invention a sequence of an even-numbered 00 SEQ ID NO: of the Sequence Listing). In other embodiments, the isolated HA protein comprises an amino acid sequence which is at least about 50% or more homologous to one of the amino acid sequences of the invention a sequence of an even-numbered "1 0 SEQ ID NO: of the Sequence Listing) and is able to participate in the maintenance of homeostasis in C glutamicum, or to perform a function involved in the adaptation of this microorganism to different environmental conditions, or has one or more of the activities set forth in Table 1.

Alternatively, the isolated HA protein can comprise an amino acid sequence which is encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80%, or 90%, and even more preferably at least about.

96%, 97%, or 99% or more homologous, to a nucleotide sequence of one of the even-numbered SEQ ID NOs set forth in the Sequence Listing. It is also preferred that the preferred forms of HA proteins also have one or more of the HA bioactivities described herein.

The HA polypeptide, or a biologically active portion thereof, can be operatively linked to a non-HA polypeptide to form a fusion protein. In preferred embodiments, this fusion protein has an activity which differs from that of the HA protein alone. In other preferred embodiments, this fusion protein participates in the maintenance of homeostasis in C. glulamicum, or performs a function involved in the adaptation of this microorganism to different environmental conditions. In particularly preferred embodiments, integration of this fusion protein into a host cell modulates production of a desired compound from the cell.

In another aspect, the invention provides methods for screening molecules which modulate the activity of an HA protein, either by interacting with the protein itself or a (4 substrate or binding partner of the HA protein, or by modulating the transcription or Stranslation of an HA nucleic acid molecule of the invention.

Another aspect of the invention pertains to a method for producing a fine C1 chemical. This method involves the culturing of a cell containing a vector directing the expression of an HA nucleic acid molecule of the invention, such that a fine chemical is N produced. In a preferred embodiment, this method further includes the step of obtaining 00 0a cell containing such a vector, in which a cell is transfected with a vector directing the expression of an HA nucleic acid. In another preferred embodiment, this method further includes the step of recovering the fine chemical from the culture. In a particularly 10 preferred embodiment, the cell is from the genus Corynebacterium or Brevibacterium, or is selected from those strains set forth in Table 3.

Another aspect of the invention pertains to methods for modulating production of a molecule from a microorganism. Such methods include contacting the cell with an agent which modulates HA protein activity or HA nucleic acid expression such that a cell associated activity is altered relative to this same activity in the absence of the agent. In a preferred embodiment, the cell is modulated for one or more C. glutamicum processes involved in cell wall biosynthesis or rearrangements, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or enzymatic or proteolytic activities. The agent which modulates HA protein activity can be an agent which stimulates HA protein activity or HA nucleic acid expression.

Examples of agents which stimulate HA protein activity or HA nucleic acid expression include small molecules, active HA proteins, and nucleic acids encoding HA proteins that have been introduced into the cell. Examples of agents which inhibit HA activity or expression include small molecules and antisense HA nucleic acid molecules.

Another aspect of the invention pertains to methods for modulating yields of a desired compound from a cell, involving the introduction of a wild-type or mutant HA gene into a cell, either maintained on a separate plasmid or integrated into the genome of the host cell. If integrated into the genome, such integration can be random, or it can take place by homologous recombination such that the native gene is replaced by the introduced copy, causing the production of the desired compound from the cell to be modulated. In a preferred embodiment, said yields are increased. In another preferred embodiment, said chemical is a fine chemical. In a particularly preferred embodiment, -11- (N said fine chemical is an amino acid. In especially preferred embodiments, said amino Sacid is L-lysine.

C Detailed Description of the Invention The present invention provides HA nucleic acid and protein molecules which are involved in C glutamicum cell wall biosynthesis or rearrangements, metabolism of 00 inorganic compounds, modification or degradation of aromatic or aliphatic compounds, C or that have a C. glutamicum enzymatic or proteolytic activity. The molecules of the 0invention may be utilized in the modulation of production of fine chemicals from CI 10 microorganisms, such as C. glutamicum, either directly where overexpression or optimization of activity of a protein involved in the production of a fine chemical an enzyme) has a direct impact on the yield, production, and/or efficiency of production of a fine chemical from the modified C. glutamicum), or an indirect impact which nonetheless results in an increase of yield, production, and/or efficiency of production of the desired compound where modulation of the activity or number of copies of a C. glutamicum aromatic or aliphatic modification or degradation protein results in an increase in the viability of C. glutamicum cells, which in turn permits increased production in a large-scale culture setting). Aspects of the invention are further explicated below.

I. Fine Chemicals The term 'fine chemical' is art-recognized and includes molecules produced by an organism which have applications in various industries, such as, but not limited to, the pharmaceutical, agriculture, and cosmetics industries. Such compounds include organic acids, such as tartaric acid, itaconic acid, and diaminopimelic acid, both proteinogenic and non-proteinogenic amino acids, purine and pyrimidine bases, nucleosides, and nucleotides (as described e.g. in Kuninaka, A. (1996) Nucleotides and related compounds, p. 561-612, in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, and references contained therein), lipids, both saturated and unsaturated fatty acids arachidonic acid), diols propane diol, and butane diol), carbohydrates hyaluronic acid and trehalose), aromatic compounds aromatic amines, vanillin, and indigo), vitamins and cofactors (as described in Ullmann's Encyclopedia of -12- (N Industrial Chemistry, vol. A27, "Vitamins", p. 443-613 (1996) VCH: Weinheim and Sreferences therein; and Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and i1 Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press, (1995)), enzymes, 0 polyketides (Cane et al. (1998) Science 282: 63-68), and all other chemicals described in 00 0Gutcho (1983) Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0C 0818805086 and references therein. The metabolism and uses of certain of these fine Schemicals are further explicated below.

A. Amino Acid Metabolism and Uses Amino acids comprise the basic structural units of all proteins, and as such are essential for normal cellular functioning in all organisms. The term "amino acid" is artrecognized. The proteinogenic amino acids, of which there are 20 species, serve as structural units for proteins, in which they are linked by peptide bonds, while the nonproteinogenic amino acids (hundreds of which are known) are not normally found in proteins (see Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97 VCH: Weinheim (1985)). Amino acids may be in the D- or L- optical configuration, though Lamino acids are generally the only type found in naturally-occurring proteins.

Biosynthetic and degradative pathways of each of the 20 proteinogenic amino acids have been well characterized in both prokaryotic and eukaryotic cells (see, for example, Stryer, L. Biochemistry, 3 r d edition, pages 578-590 (1988)). The 'essential' amino acids (histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine), so named because they are generally a nutritional requirement due to the complexity of their biosyntheses, are readily converted by simple biosynthetic pathways to the remaining 11 'nonessential' amino acids (alanine, arginine, asparagine, aspartate, cysteine, glutamate, glutamine, glycine, proline, serine, and tyrosine). Higher animals do retain the ability to synthesize some of these amino acids, but the essential amino acids must be supplied from the diet in order for normal protein synthesis to occur.

Aside from their function in protein biosynthesis, these amino acids are interesting chemicals in their own right, and many have been found to have various applications in the food, feed, chemical, cosmetics, agriculture, and pharmaceutical 13- C industries. Lysine is an important amino acid in the nutrition not only of humans, but also ofmonogastric animals such as poultry and swine. Glutamate is most commonly Sused as a flavor additive (mono-sodium glutamate, MSG) and is widely used throughout c the food industry, as are aspartate, phenylalanine, glycine, and cysteine. Glycine, Lmethionine and tryptophan are all utilized in the pharmaceutical industry. Glutamine, valine, leucine, isoleucine, histidine, arginine, proline, serine and alanine are of use in Sboth the pharmaceutical and cosmetics industries. Threonine, tryptophan, and D/ L- Smethionine are common feed additives. (Leuchtenberger, W. (1996) Amino aids Stechnical production and use, p. 466-502 in Rehm et al. (eds.) Biotechnology vol. 6, i 10 chapter 14a, VCH: Weinheim). Additionally, these amino acids have been found to be useful as precursors for the synthesis of synthetic amino acids and proteins, such as Nacetylcysteine, S-carboxymethyl-L-cysteine, (S)-5-hydroxytryptophan, and others described in Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97, VCH: Weinheim, 1985.

The biosynthesis of these natural amino acids in organisms capable of producing them, such as bacteria, has been well characterized (for review of bacterial amino acid biosynthesis and regulation thereof, see Umbarger, H.E.(1978) Ann. Rev.

Biochem. 47: 533-606). Glutamate is synthesized by the reductive amination of aketoglutarate, an intermediate in the citric acid cycle. Glutamine, proline, and arginine are each subsequently produced from glutamate. The biosynthesis of serine is a threestep process beginning with 3-phosphoglycerate (an intermediate in glycolysis), and resulting in this amino acid after oxidation, transamination, and hydrolysis steps. Both cysteine and glycine are produced from serine; the former by the condensation of homocysteine with serine, and the latter by the transferal of the side-chain P-carbon atom to tetrahydrofolate, in a reaction catalyzed by serine transhydroxymethylase.

Phenylalanine, and tyrosine are synthesized from the glycolytic and pentose phosphate pathway precursors erythrose 4-phosphate and phosphoenolpyruvate in a 9-step biosynthetic pathway that differ only at the final two steps after synthesis of prephenate.

Tryptophan is also produced from these two initial molecules, but its synthesis is an 11step pathway. Tyrosine may also be synthesized from phenylalanine, in a reaction catalyzed by phenylalanine hydroxylase. Alanine, valine, and leucine are all biosynthetic products of pyruvate, the final product of glycolysis. Aspartate is formed -14r, from oxaloacetate, an intermediate of the citric acid cycle. Asparagine, methionine, Sthreonine, and lysine are each produced by the conversion of aspartate. Isoleucine is formed from threonine. A complex 9-step pathway results in the production of histidine c from 5-phosphoribosyl-l-pyrophosphate, an activated sugar.

Amino acids in excess of the protein synthesis needs of the cell cannot be stored, 0 and are instead degraded to provide intermediates for the major metabolic pathways of 00 0 the cell (for review see Stryer, L. Biochemistry 3 rd ed. Ch. 21 "Amino Acid Degradation

O

CI and the Urea Cycle" p. 495-516 (1988)). Although the cell is able to convert unwanted O amino acids into useful metabolic intermediates, amino acid production is costly in

O

CI 10 terms of energy, precursor molecules, and the enzymes necessary to synthesize them.

Thus it is not surprising that amino acid biosynthesis is regulated by feedback inhibition, in which the presence of a particular amino acid serves to slow or entirely stop its own production (for overview of feedback mechanisms in amino acid biosynthetic pathways, see Stryer, L. Biochemistry, 3 rd ed. Ch. 24: "Biosynthesis of Amino Acids and Heme" p.

575-600 (1988)). Thus, the output of any particular amino acid is limited by the amount of that amino acid present in the cell.

B. Vitamin, Cofactor, and Nutraceutical Metabolism and Uses Vitamins, cofactors, and nutraceuticals comprise another group of molecules which the higher animals have lost the ability to synthesize and so must ingest, although they are readily synthesized by other organisms such as bacteria. These molecules are either bioactive substances themselves, or are precursors of biologically active substances which may serve as electron carriers or intermediates in a variety of metabolic pathways. Aside from their nutritive value, these compounds also have significant industrial value as coloring agents, antioxidants, and catalysts or other processing aids. (For an overview of the structure, activity, and industrial applications of these compounds, see, for example, Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996.) The term "vitamin" is artrecognized, and includes nutrients which are required by an organism for normal functioning, but which that organism cannot synthesize by itself. The group of vitamins may encompass cofactors and nutraceutical compounds. The language "cofactor" includes nonproteinaceous compounds required for a normal enzymatic activity to r-N occur. Such compounds may be organic or inorganic; the cofactor molecules of the Dinvention are preferably organic. The term "nutraceutical" includes dietary supplements having health benefits in plants and animals, particularly humans. Examples of such Nmolecules are vitamins, antioxidants, and also certain lipids polyunsaturated fatty acids).

O The biosynthesis of these molecules in organisms capable of producing them, 00 Ssuch as bacteria, has been largely characterized (Ullman's Encyclopedia of Industrial N Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996; Michal, G. (1999) SBiochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley C 10 Sons; Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept.

1-3, 1994 at Penang, Malaysia, AOCS Press: Champaign, IL X, 374 S).

Thiamin (vitamin B 1 is produced by the chemical coupling ofpyrimidine and thiazole moieties. Riboflavin (vitamin 82) is synthesized from (GTP) and ribose-5'-phosphate. Riboflavin, in turn, is utilized for the synthesis of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). The family of compounds collectively termed 'vitamin B 6 pyridoxine, pyridoxamine, pyridoxaand the commercially used pyridoxin hydrochloride) are all derivatives of the common structural unit, 5-hydroxy-6-methylpyridine. Pantothenate (pantothenic acid, (R)-(+)-N-(2,4-dihydroxy-3,3-dimethyl-I-oxobutyl)-P-alanine) can be produced either by chemical synthesis or by fermentation. The final steps in pantothenate biosynthesis consist of the ATP-driven condensation of p-alanine and pantoic acid. The enzymes responsible for the biosynthesis steps for the conversion to pantoic acid, to Palanine and for the condensation to panthotenic acid are known. The metabolically active form of pantothenate is Coenzyme A, for which the biosynthesis proceeds in enzymatic steps. Pantothenate, pyridoxal-5'-phosphate, cysteine and ATP are the precursors of Coenzyme A. These enzymes not only catalyze the formation of panthothante, but also the production of (R)-pantoic acid, (R)-pantolacton, panthenol (provitamin Bs), pantetheine (and its derivatives) and coenzyme A.

Biotin biosynthesis from the precursor molecule pimeloyl-CoA in microorganisms has been studied in detail and several of the genes involved have been -16r, identified. Many of the corresponding proteins have been found to also be involved in )Fe-cluster synthesis and are members of the nifS class of proteins. Lipoic acid is derived from octanoic acid, and serves as a coenzyme in energy metabolism, where it becomes part of the pyruvate dehydrogenase complex and the a-ketoglutarate dehydrogenase complex. The folates are a group of substances which are all derivatives Sof folic acid, which is turn is derived from L-glutamic acid, p-amino-benzoic acid and 6- 00 Smethylpterin. The biosynthesis of folic acid and its derivatives, starting from the C metabolism intermediates guanosine-5'-triphosphate (GTP), L-glutamic acid and p- Samino-benzoic acid has been studied in detail in certain microorganisms.

C1 10 Corrinoids (such as the cobalamines and particularly vitamin B 1 2 and porphyrines belong to a group of chemicals characterized by a tetrapyrole ring system.

The biosynthesis of vitamin B 2 is sufficiently complex that it has not yet been completely characterized, but many of the enzymes and substrates involved are now known. Nicotinic acid (nicotinate), and nicotinamide are pyridine derivatives which are also termed 'niacin'. Niacin is the precursor of the important coenzymes NAD (nicotinamide adenine dinucleotide) and NADP (nicotinamide adenine dinucleotide phosphate) and their reduced forms.

The large-scale production of these compounds has largely relied on cell-free chemical syntheses, though some of these chemicals have also been produced by largescale culture of microorganisms, such as riboflavin, Vitamin B 6 pantothenate, and biotin. Only Vitamin B 12 is produced solely by fermentation, due to the complexity of its synthesis. In vitro methodologies require significant inputs of materials and time, often at great cost.

C. Purine, Pyrimidine, Nucleoside and Nucleotide Metabolism and Uses Purine and pyrimidine metabolism genes and their corresponding proteins are important targets for the therapy of tumor diseases and viral infections. The language "purine" or "pyrimidine" includes the nitrogenous bases which are constituents of nucleic acids, co-enzymes, and nucleotides. The term "nucleotide" includes the basic structural units of nucleic acid molecules, which are comprised of a nitrogenous base, a pentose sugar (in the case of RNA, the sugar is ribose; in the case of DNA, the sugar is D-deoxyribose), and phosphoric acid. The language "nucleoside" includes molecules -17r, which serve as precursors to nucleotides, but which are lacking the phosphoric acid Dmoiety that nucleotides possess. By inhibiting the biosynthesis of these molecules, or their mobilization to form nucleic acid molecules, it is possible to inhibit RNA and DNA synthesis; by inhibiting this activity in a fashion targeted to cancerous cells, the ability of tumor cells to divide and replicate may be inhibited. Additionally, there are 0 nucleotides which do not form nucleic acid molecules, but rather serve as energy stores 00 0 AMP) or as coenzymes FAD and NAD).

CN Several publications have described the use of these chemicals for these medical

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Sindications, by influencing purine and/or pyrimidine metabolism Christopherson, C 10 R.I. and Lyons, S.D. (1990) "Potent inhibitors of de novo pyrimidine and purine biosynthesis as chemotherapeutic agents." Med. Res. Reviews 10: 505-548). Studies of enzymes involved in purine and pyrimidine metabolism have been focused on the development of new drugs which can be used, for example, as immunosuppressants or anti-proliferants (Smith, (1995) "Enzymes in nucleotide synthesis." Curr. Opin.

Struct. Biol. 5: 752-757; (1995) Biochem Soc. Transact. 23: 877-902). However, purine and pyrimidine bases, nucleosides and nucleotides have other utilities: as intermediates in the biosynthesis of several fine chemicals thiamine, S-adenosyl-methionine, folates, or riboflavin), as energy carriers for the cell ATP or GTP), and for chemicals themselves, commonly used as flavor enhancers IMP or GMP) or for several medicinal applications (see, for example, Kuninaka, A. (1996) Nucleotides and Related Compounds in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, p. 561- 612). Also, enzymes involved in purine, pyrimidine, nucleoside, or nucleotide metabolism are increasingly serving as targets against which chemicals for crop protection, including fungicides, herbicides and insecticides, are developed.

The metabolism of these compounds in bacteria has been characterized (for reviews see, for example, Zalkin, H. and Dixon, J.E. (1992) "de novo purine nucleotide biosynthesis", in: Progress in Nucleic Acid Research and Molecular Biology, vol. 42, Academic Press:, p. 259-287; and Michal, G. (1999) "Nucleotides and Nucleosides", Chapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York). Purine metabolism has been the subject of intensive research, and is essential to the normal functioning of the cell. Impaired purine metabolism in higher animals can cause severe disease, such as gout. Purine nucleotides are synthesized from -18-

C

N ribose-5-phosphate, in a series of steps through the intermediate compound Sphosphate (IMP), resulting in the production of guanosine-5'-monophosphate (GMP) or (AMP), from which the triphosphate forms utilized as c, nucleotides are readily formed. These compounds are also utilized as energy stores, so their degradation provides energy for many different biochemical processes in the cell.

O Pyrimidine biosynthesis proceeds by the formation of uridine-5'-monophosphate (UMP) 00 Sfrom ribose-5-phosphate. UMP, in turn, is converted to cytidine-5'-triphosphate (CTP).

C The deoxy- forms of all of these nucleotides are produced in a one step reduction Sreaction from the diphosphate ribose form of the nucleotide to the diphosphate C1 10 deoxyribose form of the nucleotide. Upon phosphorylation, these molecules are able to participate in DNA synthesis.

D. Trehalose Metabolism and Uses Trehalose consists of two glucose molecules, bound in a, a-1,1 linkage. It is commonly used in the food industry as a sweetener, an additive for dried or frozen foods, and in beverages. However, it also has applications in the pharmaceutical, cosmetics and biotechnology industries (see, for example, Nishimoto et al., (1998) U.S.

Patent No. 5,759,610; Singer, M.A. and Lindquist, S. (1998) Trends Biotech. 16: 460- 467; Paiva, C.L.A. and Panek, A.D. (1996) Biotech. Ann. Rev. 2: 293-314; and Shiosaka, M. (1997) J. Japan 172: 97-102). Trehalose is produced by enzymes from many microorganisms and is naturally released into the surrounding medium, from which it can be collected using methods known in the art.

II. Maintenance of Homeostasis in C. glutamicum and Environmental Adaptation The metabolic and other biochemical processes by which cells function are sensitive to environmental conditions such as temperature, pressure, solute concentration, and availability of oxygen. When one or more such environmental condition is perturbed or altered in a fashion that is incompatible with the normal functioning of these cellular processes, the cell must act to maintain an intracellular environment which will permit them to occur despite the hostile extracellular environment. Gram positive bacterial cells, such as C glutamicum cells, have a number of mechanisms by which internal homeostasis may be maintained despite unfavorable -19r, extracellular conditions. These include a cell wall, proteins which are able to degrade Spossibly toxic aromatic and aliphatic compounds, mechanisms of proteolysis whereby misfolded or misregulated proteins may be rapidly destroyed, and catalysts which permit intracellular reactions to occur which would not normally take place under the conditions optimal for bacterial growth.

Aside from merely surviving in a hostile environment, bacterial cells C.

00 oO glutamicum cells) are also frequently able to adapt such that they are able to take N, advantage of such conditions. For example, cells in an environment lacking desired

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carbon sources may be able to adapt to growth on a less-suitable carbon source. Also, 10 cells may be able to utilize less desirable inorganic compounds when the commonly utilized ones are unavailable. C. glutamicum cells possess a number of genes which permit them to adapt to utilize inorganic and organic molecules which they would normally not encounter under optimal growth conditions as nutrients and precursors for metabolism. Aspects of cellular processes involved in homeostasis and adaptation are further explicated below.

A. Modification and Degradation ofAromalic and Aliphatic Compounds Bacterial cells are routinely exposed to a variety of aromatic and aliphatic compounds in nature. Aromatic compounds are organic molecules having a cyclic ring structure, while aliphatic compounds are organic molecules having open chain structures rather than ring structures. Such compounds may arise as by-products of industrial processes benzene or toluene), but may also be produced by certain microorganisms alcohols). Many of these compounds are toxic to cells, particularly the aromatic compounds, which are highly reactive due to the high-energy ring structure. Thus, certain bacteria have developed mechanisms by which they are able to modify or degrade these compounds such that they are no longer hazardous to the cell. Cells may possess enzymes that are able to, for example, hydroxylate, isomerize, or methylate aromatic or aliphatic compounds such that they are either rendered less toxic, or such that the modified form is able to'be processed by standard cellular waste and degradation pathways. Also, cells may possess enzymes which are able to specifically degrade one or more such potentially hazardous substance, thereby protecting the cell. Principles and examples of these types of modification and degradation processes in bacteria are described in several publications, r' Sahm, H. (1999) "Procaryotes in Industrial Production" in Lengeler, J.W. et al., eds.

DBiology of the Procaryotes, Thieme Verlag: Stuttgart; and Schlegel, H.G. (1992) Allgemeine Mikrobiologie, Thieme: Stuttgart).

NAside from simply inactivating hazardous aromatic or aliphatic compounds, many bacteria have evolved to be able to utilize these compounds as carbon sources for continued O metabolism when the preferred carbon sources of the cell are not available. For example, 00 0 Pseudomonas strains able to utilize toluene, benzene, and 1,10-dichlorodecane as carbon 0 N sources are known (Chang, B.V. et al. (1997) Chemosphere 35(12): 2807-2815; Wischnak,

ID

SC. et al. (1998) Appl. Environ. Microbiol. 64(9): 3507-3511; Churchill, S.A. et al. (1999) C 10 Appl. Environ. Microbiol. 65(2): 549-552). There are similar examples from many other bacterial species which are known in the art.

The ability of certain bacteria to modify or degrade aromatic and aliphatic compounds has begun to be exploited. Petroleum is a complex mixture of chemicals which includes aliphatic molecules and aromatic compounds. By applying bacteria having the ability to degrade or modify these toxic compounds to an oil spill, for example, it is possible to eliminate much of the environmental damage with high efficiency and low cost (see, for example, Smith, M.R. (1990) "The biodegradation of aromatic hydrocarbons by bacteria" Biodegradation 191-206; and Suyama, T. et al. (1998) "Bacterial isolates degrading aliphatic polycarbonates," FEMS Microbiol. Lett. 161(2): 255-261).

B. Metabolism of norganic Compounds Cells bacterial cells) contain large quantities of different molecules, such as water, inorganic ions, and organic substances proteins, sugars, and other macromolecules). The bulk of the mass of a typical cell consists of only 4 types of atoms: carbon, oxygen, hydrogen, and nitrogen. Although they represent a smaller percentage of the content of a cell, inorganic substances are equally as important to the proper functioning of the cell. Such molecules include phosphorous, sulfur, calcium, magnesium, iron, zinc, manganese, copper, molybdenum, tungsten, and cobalt. Many of these compounds are critical for the construction of important molecules, such as nucleotides (phosphorous) and amino acids (nitrogen and sulfur). Others of these inorganic ions serve as cofactors for enzymic reactions or contribute to osmotic pressure. All such molecules must be taken up by the bacterium from the surrounding environment.

-21 C For each of these inorganic compounds it is desirable for the bacterium to take up Sthe form which can be most readily used by the standard metabolic machinery of the cell.

However, the bacterium may encounter environments in which these preferred forms are not

C

readily available. In order to survive under these circumstances, it is important for bacteria to have additional biochemical mechanisms which are able to convert less metabolically 0 active but readily available forms of these inorganic compounds to ones which may be used 00 Sin cellular metabolism. Bacteria frequently possess a number of genes encoding enzymes Cr for this purpose, which are not expressed unless the desired inorganic species are not 0available. Thus, these genes for the metabolism of various inorganic compounds serve as C 10 another tool which bacteria may use to adapt to suboptimal environmental conditions.

After carbon, the most important element in the cell is nitrogen. A typical bacterial cell contains between 12-15% nitrogen. It is a constituent of amino acids and nucleotides, as well as many other important molecules in the cell. Further, nitrogen may serve as a substitute for oxygen as a terminal electron acceptor in energy metabolism. Good sources of nitrogen include many organic and inorganic compounds, such ammonia gas or ammonia salts NH 4 CI, (NH 4 2

SO

4 or NH 4 0H), nitrates, urea, amino acids, or complex nitrogen sources like corn steep liquor, soy bean flour, soy bean protein, yeast extract, meat extract, etc. Ammonia nitrogen is fixed by the action of particular enzymes: glutamate dehydrogenase, glutamine synthase, and glutamine-2-oxoglutarate aminotransferase. The transfer of amino-nitrogen from one organic molecule to another is accomplished by the aminotransferases, a class of enzymes which transfer one amino group from an alpha-amino acid to an alpha-keto acid. Nitrate may be reduced via nitrate reductase, nitrite reductase, and further redox enzymes until it is converted to molecular nitrogen or ammonia, which may be readily utilized by the cell in standard metabolic pathways.

Phosphorous is typically found intracellularly in both organic and inorganic forms, and may be taken up by the cell in either of these forms as well, though most microorganisms preferentially take up inorganic phosphate. The conversion of organic phosphate to a form which the cell can utilize requires the action of phosphatases phytases, which hydrolyze phyate-yielding phosphate and inositol derivatives). Phosphate is a key element in the synthesis of nucleic acids, and also has a significant role in cellular energy metabolism in the synthesis of ATP, ADP, and AMP).

-22- Sulfur is a requirement for the synthesis of amino acids methionine and Dcysteine), vitamins thiamine, biotin, and lipoic acid) and iron sulfur proteins. Bacteria obtain sulfur primarily from inorganic sulfate, though thiosulfate, sulfite, and sulfide are also commonly utilized. Under conditions where these compounds may not be readily available, many bacteria express genes which enable them to utilize sulfonate compounds 0 such as 2-aminosulfonate (taurine) (Kertesz, M.A. (1993) "Proteins induced by sulfate 00 0limitation in Escherichia coli, Pseudomonas putida, or Staphylococcus aureus." J.

N Bacteriol. 175: 1187-1190).

SOther inorganic atoms, metal or calcium ions, are also critical for the N 10 viability of cells. Iron, for example, plays a key role in redox reactions and is a cofactor of iron-sulfur proteins, heme proteins, and cytochromes. The uptake of iron into bacterial cells may be accomplished by the action of siderophores, chelating agents which bind extracellular iron ions and translocate them to the interior of the cell. For reference on the metabolism of iron and other inorganic compounds, see: Lengeler et al.

(1999) Biology of Prokaryotes, Thieme Verlag: Stuttgart; Neidhardt, F.C. et al., eds.

Escherichia coli and Salmonella. ASM Press: Washington, Sonenshein, A.L. et al., eds. (199?) Bacillus subtilis and Other Gram-Positive Bacteria, ASM Press: Washington, Voet, D. and Voet, J.G. (1992) Biochemie, VCH: Weinheim; Brock, T.D. and Madigan, M.T. (1991) Biology of Microorgansisms, 6 th ed. Prentice Hall: Englewood Cliffs, p. 267-269; Rhodes, P.M. and Stanbury, P.F. Applied Microbial Physiology A Practical Approach, Oxford Univ. Press: Oxford.

C. Enzymes and Proteolysis The intracellular conditions for which bacteria such as C. glutamicum are optimized are frequently not conditions under which many biochemical reactions would normally take place. In order to make such reactions proceed under physiological conditions, cells utilize enzymes. Enzymes are proteinaceous biological catalysts, spatially orienting reacting molecules or providing a specialized environment such that the energy barrier to a biochemical reaction is lowered. Different enzymes catalyze different reactions, and each enzyme may be the subject of transcriptional, translational, or posttranslational regulation such that the reaction will only take place under appropriate conditions and at specified times. Enzymes may contribute to the -23- C1 degradation the proteases), synthesis the synthases), or modification transferases or isomerases) of compounds, all of which enable the production of necessary compounds within the cell. This, in turn, contributes to the maintenance of CN cellular homeostasis.

However, the fact that enzymes are optimized for activity under the physiological conditions at which the bacterium is most viable means that when 00 environmental conditions are perturbed, there is a significant possibility that enzyme activity will also be perturbed. For example, changes in temperature may result in Saberrantly folded proteins, and the same is true for changes ofpH protein folding is 10 largely dependent on electrostatic and hydrophobic interactions of amino acids within the polypeptide chain, so any alteration to the charges on individual amino acids (as might be brought about by a change in cellular pH) may have a profound effect on the ability of the protein to correctly fold. Changes in temperature effectively change the amount of kinetic energy that the polypeptide molecule possesses, which affects the ability of the polypeptide to settle into a correctly folded, energetically stable configuration. Misfolded proteins may be harmful to the cell for two reasons. First, the aberrantly folded protein may have a similarly aberrant activity, or no activity whatsoever. Second, misfolded proteins may lack the conformational regions necessary for proper regulation by other cellular systems and thus may continue to be active but in an uncontrolled fashion.

The cell has a mechanism by which misfolded enzymes and regulatory proteins may be rapidly destroyed before any damage occurs to the cell: proteolysis. Proteins such as those of the la/lon family and those of the Clp family specifically recognize and degrade misfolded proteins (see, Sherman, Goldberg, A.L. (1999) EXS 77: 57-78 and references therein and Porankiewicz J. (1999) Molec. Microbiol. 32(3): 449- 58, and references therein; Neidhardt, et al. (1996) E. coli and Salmonella, ASM Press: Washington, D.C. and references therein; and Pritchard, and Coolbear, T.

(1993) FEMS Microbiol. Rev. 12(1-3): 179-206 and references therein). These enzymes bind to misfolded or unfolded proteins and degrade them in an ATP-dependent manner.

Proteolysis thus serves as an important mechanism employed by the cell to prevent damage to normal cellular functions upon environmental changes, and it further permits -24- (N cells to survive under conditions and in environments which would otherwise be toxic Sdue to misregulated and/or aberrant enzyme or regulatory activity.

Proteolysis also has important functions in the cell under optimal environmental C conditions. Within normal metabolic processes, proteases aid in the hydrolysis of peptide bonds, in the catabolism of complex molecules to provide necessary degradation products, and in protein modification. Secreted proteases play an important role in the 00 catabolism of external nutrients even prior to the entry of these compounds into the cell.

SFurther, proteolytic activity itself may serve regulatory functions; sporulation in B.

subtilis and cell cycle progression in Caulobacter spp. are known to be regulated by key CI 10 proteolytic events in each of these species (Gottesman, S. (1999) Curr. Opin. Microbiol.

142-147). Thus, proteolytic processes are key for cellular survival under both suboptimal and optimal environmental conditions, and contribute to the overall maintenance of homeostasis in cells.

D. Cell Wall Production and Rearrangements While the biochemical machinery of the cell may be able to readily adapt to different and possibly unfavorable environments, cells still require a general mechanism by which they may be protected from the environment. For many bacteria, the cell wall affords such protection, and also plays roles in adhesion, cell growth and division, and transport of desired solutes and waste materials.

In order to function, cells require intracellular concentrations of metabolites and other molecules that are substantially higher than those of the surrounding media. Since these metabolites are largely prevented from leaving the cell due to the presence of the hydrophobic membrane, the tendency of the system is for water molecules to enter the cell from the external medium such that the interior concentrations of solutes match the exterior concentrations. Water molecules are readily able to cross the cellular membrane, and this membrane is not able to withstand the resulting swelling and pressure, which may lead to osmotic lysis of the cell. The rigidity of the cell wall greatly improves the ability of the cell to tolerate these pressures, and offers a further barrier to the unwanted diffusion of these metabolites and desired solutes from the cell. Similarly, the cell wall also serves to prevent unwanted material from entering the cell.

(N1 The cell wall also participates in a number of other cellular processes, such as Sadhesion and cell growth and division. Due to the fact that the cell wall completely surrounds the cell, any interaction of the cell with its surroundings must be mediated by the C, cell wall. Thus, the cell wall must participate in any adherence of the cell to other cells and to desired surfaces. Further, the cell cannot grow or divide without concomitant changes in 0 the cell wall. Since the protection that the wall affords requires its presence during growth, 00 0morphogenesis and multiplication, one of the key steps in cell division is cell wall synthesis C within the cell such that a new cell divides from the old. Thus, frequently cell wall

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0biosynthesis is regulated in tandem with cell growth and cell division (see, Sonenshein, C1 10 A.L. et al, eds. (1993) Bacillus sublilis and Other Gram-Positive Bacteria, ASM: Washington, The structure of the cell wall varies between gram-positive and gram-negative bacteria. However, in both types, the fundamental structural unit of the wall remains similar: an overlapping lattice of two polysaccharides, N-acetyl glucosamine (NAG) and Nacetyl muramic acid (NAM) which are cross-linked by amino acids (most commonly Lalanine, D-glutamate, diaminopimelic acid, and D-alanine),. termed 'peptidoglycan'. The processes involved in the synthesis of the cell wall are known (see, Michal, ed.

(1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York).

In gram-negative bacteria, the inner cellular membrane is coated by a single-layered peptidoglycan (approximately 10 nm thick), termed the murein-sacculus. This peptidoglycan structure is very rigid, and its structure determines the shape of the organism.

The outer surface of the murein-sacculus is covered with an outer membrane, containing porins and other membrane proteins, phospholipids, and lipopolysaccharides. To maintain a tight association with the outer membrane, the gram-negative cell wall also has interspersed lipid molecules which serve to anchor it to the surrounding membrane.

In gram-positive bacteria, such as Corynebacterium glutamicum, the cytoplasmic membrane is covered by a multi-layered peptidoglycan, which ranges from 20-80 nm in thickness (see, Lengeler et al. (1999) Biology of Prokaryotes Thieme Verlag: Stuttgart, p. 913-918, p. 875-899, and p. 88-109 and references therein). The gram-positive cell wall also contains teichoic acid, a polymer of glycerol or ribitol linked through phosphate groups.

Teichoic acid is also able to associate with amino acids, and forms covalent bonds with -26- (N1 muramic acid. Also present in the cell wall may be lipoteichoic acids and teichuronic acids.

If present, cellular surface structures such as flagella or capsules will be anchored in this layer as well.

III. Elements and Methods of the Invention 0 The present invention is based, at least in part, on the discovery of novel 00 0molecules, referred to herein as HA nucleic acid and protein molecules, which C participate in the maintenance of homeostasis in C. glutamicum, or which perform a 0function involved in the adaptation of this microorganism to different environmental CI 10 conditions. In one embodiment, the HA molecules participate in C glutamicum cell wall biosynthesis or rearrangements, in the metabolism of inorganic compounds, in the modification or degradation of aromatic or aliphatic compounds, or have an enzymatic or proteolytic activity. In a preferred embodiment, the activity of the HA molecules of the present invention with regard to C. glutamicum cell wall biosynthesis or rearrangements, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or enzymatic or proteolytic activity has an impact on the production of a desired fine chemical by this organism. In a particularly preferred embodiment, the HA molecules of the invention are modulated in activity, such that the C. glutamicum cellular processes in which the HA molecules participate C.

glutamicum cell wall biosynthesis or rearrangements, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or enzymatic or proteolytic activity) are also altered in activity, resulting either directly or indirectly in a modulation of the yield, production, and/or efficiency of production of a desired fine chemical by C. glutamicum.

The language, "HA protein" or "HA polypeptide" includes proteins which participate in a number of cellular processes related to C. glutamicum homeostasis or the ability of C. glutamicum cells to adapt to unfavorable environmental conditions. For example, an HA protein may be involved in C. glutamicum cell wall biosynthesis or rearrangements, in the metabolism of inorganic compounds in C. glutamicum, in the modification or degradation of aromatic or aliphatic compounds in C. glutamicum, or have a C. glutamicum enzymatic or proteolytic activity. Examples of HA proteins include those encoded by the HA genes set forth in Table 1 and by the odd-numbered -27r, SEQ ID NOs. The terms "HA gene" or "HA nucleic acid sequence" include nucleic Sacid sequences encoding an HA protein, which consist of a coding region and also corresponding untranslated 5' and 3' sequence regions. Examples of HA genes include those set forth in Table 1. The terms "production" or "productivity" are art-recognized and include the concentration of the fermentation product (for example, the desired fine chemical) formed within a given time and a given fermentation volume kg product 00 per hour per liter). The term "efficiency of production" includes the time required for a particular level of production to be achieved (for example, how long it takes for the cell Sto attain a particular rate of output of a fine chemical). The term "yield" or 10 "product/carbon yield" is art-recognized and includes the efficiency of the conversion of the carbon source into the product fine chemical). This is generally written as, for example, kg product per kg carbon source. By increasing the yield or production of the compound, the quantity of recovered molecules, or of useful recovered molecules of that compound in a given amount of culture over a given amount of time is increased. The terms "biosynthesis" or a "biosynthetic pathway" are art-recognized and include the synthesis of a compound, preferably an organic compound, by a cell from intermediate compounds in what may be a multistep and highly regulated process. The terms "degradation" or a "degradation pathway" are art-recognized and include the breakdown of a compound, preferably an organic compound, by a cell to degradation products (generally speaking, smaller or less complex molecules) in what may be a multistep and highly regulated process. The language "metabolism" is art-recognized and includes the totality of the biochemical reactions that take place in an organism.

The metabolism of a particular compound, then, the metabolism of an amino acid such as glycine) comprises the overall biosynthetic, modification, and degradation pathways in the cell related to this compound. The term "homeostasis" is art-recognized and includes all of the mechanisms utilized by a cell to maintain a constant intracellular environment despite the prevailing extracellular environmental conditions. A nonlimiting example of such processes is the utilization of a cell wall to prevent osmotic lysis due to high intracellular solute concentrations. The term "adaptation" or "adaptation to an environmental condition" is art-recognized and includes mechanisms utilized by the cell to render the cell able to survive under nonpreferred environmental conditions (generally speaking, those environmental conditions in which one or more -28- (1,I favored nutrients are absent, or in which an environmental condition such as temperature, pH, osmolarity, oxygen percentage and the like fall outside of the optimal survival range of the cell). Many cells, including C. glutamicum cells, possess genes C encoding proteins which are expressed under such environmental conditions and which permit continued growth in such suboptimal conditions.

In another embodiment, the HA molecules of the invention are capable of 00 oO modulating the production of a desired molecule, such as a fine chemical, in a microorganism such as C. glutamicum. There are a number of mechanisms by which

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the alteration of an HA protein of the invention may directly affect the yield, production, 10 and/or efficiency of production of a fine chemical from a C. glutamicum strain incorporating such an altered protein. For example, by engineering enzymes which modify or degrade aromatic or aliphatic compounds such that these enzymes are increased or decreased in activity or number, it may be possible to modulate the production of one or more fine chemicals which are the modification or degradation products of these compounds. Similarly, enzymes involved in the metabolism of inorganic compounds provide key molecules phosphorous, sulfur, and nitrogen molecules) for the biosynthesis of such fine chemicals as amino acids, vitamins, and nucleic acids. By altering the activity or number of these enzymes in C. glutamicum, it may be possible to increase the conversion of these inorganic compounds (or to use alternate inorganic compounds) to thus permit improved rates of incorporation of inorganic atoms into these fine chemicals. Genetic engineering of C. glutamicum enzymes involved in general cellular processes may also directly improve fine chemical production, since many of these enzymes directly modify fine chemicals amino acids) or the enzymes which are involved in fine chemical synthesis or secretion.

Modulation of the activity or number of cellular proteases may also have a direct effect on fine chemical production, since many proteases may degrade fine chemicals or enzymes involved in fine chemical production or breakdown.

Further, the aforementioned enzymes which participate in aromatic/aliphatic compound modification or degradation, general biocatalysis, inorganic compound metabolism or proteolysis are each themselves fine chemicals, desirable for their activity in various in vitro industrial applications. By altering the number of copies of the gene for one or more of these enzymes in C. glutamicum it may be possible to increase the -29number of these proteins produced by the cell, thereby increasing the potential yield or CD efficiency of production of these proteins from large-scale C. glutamicum or related bacterial cultures.

The alteration of an HA protein of the invention may also indirectly affect the yield, production, and/or efficiency of production of a fine chemical from a C.

0 glulamicum strain incorporating such an altered protein. For example, by modulating Sthe activity and/or number of those proteins involved in the construction or C rearrangement of the cell wall, it may be possible to modify the structure of the cell wall itself such that the cell is able to better withstand the mechanical and other stresses C 10 present during large-scale fermentative culture. Also, large-scale growth of C.

glutamicum requires significaft cell wall production. Modulation of the activity or number of cell wall biosynthetic or degradative enzymes may allow more rapid rates of cell wall biosynthesis, which in turn may permit increased growth rates of this microorganism in culture and thereby increase the number of cells producing the desired fine chemical.

By modifying the HA enzymes of the invention, one may also indirectly impact the yield, production, or efficiency of production of one or more fine chemicals from C.

glutamicum. For example, many of the general enzymes in C. glutamicum may have a significant impact on global cellular processes regulatory processes) which in turn have a significant effect on fine chemical metabolism. Similarly, proteases, enzymes which modify or degrade possibly toxic aromatic or aliphatic compounds, and enzymes which promote the metabolism of inorganic compounds all serve to increase the viability of C. glutamicum. The proteases aid in the selective removal of misfolded or misregulated proteins, such as those that might occur under the relatively stressful environmental conditions encountered during large-scale fermentor culture. By altering these proteins, it may be possible to further enhance this activity and to improve the viability of C. glutamicum in culture. The aromatic/aliphatic modification or degradation proteins not only serve to detoxify these waste compounds (which may be encountered as impurities in culture medium or as waste products from cells themselves), but also to permit the cells to utilize alternate carbon sources if the optimal carbon source is limiting in the culture. By increasing their number and/or activity, the survival of C. glutamicum cells in culture may be enhanced. The inorganic metabolism ,11 proteins of the invention supply the cell with inorganic molecules required for all protein Sand nucleotide (among others) synthesis, and thus are critical for the overall viability of the cell. An increase in the number of viable cells producing one or more desired fine chemicals in large-scale culture should result in a concomitant increase in the yield, production, and/or efficiency of production of the fine chemical in the culture.

0 The isolated nucleic acid sequences of the invention are contained within the 00 0genome of a Corynebacterium glutamicum strain available through the American Type C1 Culture Collection, given designation ATCC 13032. The nucleotide sequence of the 0isolated C glutamicum HA DNAs and the predicted amino acid sequences of the C.

N, 10 glutamicum HA proteins are shown in the Sequence Listing as odd-numbered SEQ ID NOs and even-numbered SEQ ID NOs, respectively, respectively. Computational analyses were performed which classified and/or identified these nucleotide sequences as sequences which encode proteins that participate in C. glutamicum cell wall biosynthesis or rearrangements, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or that have a C. glutamicum enzymatic or proteolytic activity.

The present invention also pertains to proteins which have an amino acid sequence which is substantially homologous to an amino acid sequence of the invention the sequence of an even-numbered SEQ ID NO of the Sequence Listing). As used herein, a protein which has an amino acid sequence which is substantially homologous to a selected amino acid sequence is least about 50% homologous to the selected amino acid sequence, the entire selected amino acid sequence. A protein which has an amino acid sequence which is substantially homologous to a selected amino acid sequence can also be least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, or 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to the selected amino acid sequence.

The HA protein or a biologically active portion or fragment thereof of the invention can participate in the maintenance of homeostasis in C. glutamicum, or can perform a function involved in the adaptation of this microorganism to different environmental conditions, or have one or more of the activities set forth in Table 1.

Various aspects of the invention are described in further detail in the following subsections.

-31 SA. Isolated Nucleic Acid Molecules One aspect of the invention pertains to isolated nucleic acid molecules that encode HA polypeptides or biologically active portions thereof, as well as nucleic acid fragments sufficient for use as hybridization probes or primers for the identification or amplification of HA-encoding nucleic acid HA DNA). As used herein, the term 00o "nucleic acid molecule" is intended to include DNA molecules cDNA or genomic DNA) and RNA molecules mRNA) and analogs of the DNA or RNA generated using nucleotide analogs. This term also encompasses untranslated sequence located at C 10 both the 3' and 5' ends of the coding region of the gene: at least about 100 nucleotides of sequence upstream from the 5' end of the coding region and at least about nucleotides of sequence downstream from the 3'end of the coding region of the gene.

The nucleic acid molecule can be single-stranded or double-stranded, but preferably is double-stranded DNA. An "isolated" nucleic acid molecule is one which is separated from other nucleic acid molecules which are present in the natural source of the nucleic acid. Preferably, an "isolated" nucleic acid is free of sequences which naturally flank the nucleic acid sequences located at the 5' and 3' ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. For example, in various embodiments, the isolated HA nucleic acid molecule can contain less than about 5 kb, 4kb, 3kb, 2kb, 1 kb, 0.5 kb or 0.1 kb of nucleotide sequences which naturally flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived a C. glutamicum cell). Moreover, an "isolated" nucleic acid molecule, such as a DNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized.

A nucleic acid molecule of the present invention, a nucleic acid molecule having a nucleotide sequence of an odd-numbered SEQ ID NO of the Sequence Listing, or a portion thereof, can be isolated using standard molecular biology techniques and the sequence information provided herein. For example, a C. glutamicum HA DNA can be isolated from a C. glutamicum library using all or portion of one of the odd-numbered SEQ ID NO sequences of the Sequence Listing as a hybridization probe and standard hybridization techniques as described in Sambrook, Fritsh, E. and Maniatis, -32r, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor DLaboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989).

Moreover, a nucleic acid molecule encompassing all or a portion of one of the nucleic

C

acid sequences of the invention an odd-numbered SEQ ID NO:) can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this O sequence a nucleic acid molecule encompassing all or a portion of one of the 00 Snucleic acid sequences of the invention an odd-numbered SEQ ID NO of the Cl Sequence Listing) can be isolated by the polymerase chain reaction using Soligonucleotide primers designed based upon this same sequence). For example, mRNA can be isolated from normal endothelial cells by the guanidinium-thiocyanate extraction procedure of Chirgwin et al. (1979) Biochemistry .18: 5294-5299) and DNA can be prepared using reverse transcriptase Moloney MLV reverse transcriptase, available from Gibco/BRL, Bethesda, MD; or AMV reverse transcriptase, available from Seikagaku America, Inc., St. Petersburg, FL). Synthetic oligonucleotide primers for polymerase chain reaction amplification can be designed based upon one of the nucleotide sequences shown in the Sequence Listing. A nucleic acid of the invention can be amplified using cDNA or, alternatively, genomic DNA, as a template and appropriate oligonucleotide primers according to standard PCR amplification techniques. The nucleic acid so amplified can be cloned into an appropriate vector and characterized by DNA sequence analysis. Furthermore, oligonucleotides corresponding to an HA nucleotide sequence can be prepared by standard synthetic techniques, e.g., using an automated DNA synthesizer.

In a preferred embodiment, an isolated nucleic acid molecule of the invention comprises one of the nucleotide sequences shown in the Sequence Listing. The nucleic acid sequences of the invention, as set forth in the Sequence Listing, correspond to the Corynebacterium glutamicum HA DNAs of the invention. This DNA comprises sequences encoding HA proteins the "coding region", indicated in each oddnumbered SEQ ID NO: sequence in the Sequence Listing), as well as 5' untranslated sequences and 3' untranslated sequences, also indicated in each odd-numbered SEQ ID NO: in the Sequence Listing. Alternatively, the nucleic acid molecule can comprise only the coding region of any of the sequences in nucleic acid sequences of the Sequence Listing -33- CN For the purposes of this application, it will be understood that each of the nucleic acid and amino acid sequences set forth in the Sequence Listing has an identifying RXA, RXN, RXS, or RXC number having the designation "RXA," "RXN," "RXS, or (N "RXC" followed by 5 digits RXA02458, RXN00249, RXS00153, or RXC00963).

Each of the nucleic acid sequences comprises up to three parts: a 5' upstream region, a coding region, and a downstream region. Each of these three regions is identified by the 00 same RXA, RXN, RXS, or RXC designation to eliminate confusion. The recitation S"one of the odd-numbered sequences in of the Sequence Listing", then, refers to any of Sthe nucleic acid sequences in the Sequence Listing, which may also be distinguished by 10 their differing RXA, RXN, RXS, or RXC designations. The coding region of each of these sequences is translated into a corresponding amino acid sequence, which is also set forth in the Sequence Listing, as an even-numbered SEQ ID NO: immediately following the corresponding nucleic acid sequence For example, the coding region for RXA02548 is set forth in SEQ ID NO:1, while the amino acid sequence which it encodes is set forth as SEQ ID NO:2. The sequences of the nucleic acid molecules of the invention are identified by the same RXA, RXN, RXS, or RXC designations as the amino acid molecules which they encode, such that they can be readily correlated. For example, the amino acid sequences designated RXA02458, RXN00249, RXS00153, and RXC00963 are translations of the coding regions of the nucleotide sequences of nucleic acid molecules RXA02458, RXN00249, RXS00153, and RXC00963, respectively, of the correspondence between the RXA, RXN, RXS, and RXC nucleotide and amino acid sequences of the invention and their assigned SEQ ID NOs is set forth in Table 1.

Several of the genes of the invention are "F-designated genes". An F-designated gene includes those genes set forth in Table 1 which have an in front of the RXA, RXN, RXS, or RXC designation. For example, SEQ ID NO:5, designated, as indicated on Table 1, as "F RXA00249", is an F-designated gene, as are SEQ ID NOs: 11, 15, and 33 (designated on Table 1 as "F RXA02264", "F RXA02274", and "F RXA00675", respectively).

In one embodiment, the nucleic acid molecules of the present invention are not intended to include those compiled in Table 2. In the case of the dapD gene, a sequence for this gene was published in Wehrmann, et al. (1998) J. Bacteriol. 180(12): 3159- 3165. However, the sequence obtained by the inventors of the present application is -34- N, significantly longer than the published version. It is believed that the published version Srelied on an incorrect start codon, and thus represents only a fragment of the actual coding region.

In another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleic acid molecule which is a complement of one of the nucleotide sequences of the invention a sequence of an odd-numbered SEQ ID 00 NO: of the Sequence Listing), or a portion thereof. A nucleic acid molecule which is complementary to one of the nucleotide sequences of the invention is one which is Csufficiently complementary to one of the nucleotide sequences the Sequence Listing 10 the sequence of an odd-numbered SEQ ID NO:) such that it can hybridize to one of the nucleotide sequences of the invention, thereby forming a stable duplex.

In still another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing), or a portion thereof. Ranges and identity values intermediate to the above-recited ranges, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. In an additional preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes, hybridizes under stringent conditions, to one of the nucleotide sequences of the invention, or a portion thereof Moreover, the nucleic acid molecule of the invention can comprise only a portion of the coding region of the sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, for example a fragment which can be used as a probe or primer or a fragment encoding a biologically active portion of an HA protein. The nucleotide sequences determined from the cloning of the HA genes from C. glutamicum allows for the generation of probes and primers designed for use in identifying and/or cloning HA r, homologues in other cell types and organisms, as well as HA homologues from other DCorynebacteria or related species. The probe/primer typically comprises substantially purified oligonucleotide. The oligonucleotide typically comprises a region of nucleotide N, sequence that hybridizes under stringent conditions to at least about 12, preferably about 25, more preferably about 40, 50 or 75 consecutive nucleotides of a sense strand of one of the nucleotide sequences of the invention a sequence of one of the odd- 00 numbered SEQ ID NOs of the Sequence Listing), an anti-sense sequence of one of these sequences or naturally occurring mutants thereof. Primers based on a nucleotide Ssequence of the invention can be used in PCR reactions to clone HA homologues.

Cr 10 Probes based on the HA nucleotide sequences can be used to detect transcripts or genomic sequences encoding the same or homologous proteins. In preferred embodiments, the probe further comprises a label group attached thereto, e.g. the label group can be a radioisotope, a fluorescent compound, an enzyme, or an enzyme cofactor. Such probes can be used as a part of a diagnostic test kit for identifying cells which misexpress an HA protein, such as by measuring a level of an HA-encoding nucleic acid in a sample of cells, detecting HA mRNA levels or determining whether a genomic HA gene has been mutated or deleted.

In one embodiment, the nucleic acid molecule of the invention encodes a protein or portion thereof which includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence of an evennumbered SEQ ID NO of the Sequence Listing) such that the protein or portion thereof maintains the ability to participate in the maintenance of homeostasis in C. glutamicum, or to perform a function involved in the adaptation of this microorganism to different environmental conditions. As used herein, the language "sufficiently homologous" refers to proteins or portions thereof which have amino acid sequences which include a minimum number of identical or equivalent an amino acid residue which has a similar side chain as an amino acid residue in a sequence of one of the even-numbered SEQ ID NOs of the Sequence Listing) amino acid residues to an amino acid sequence of the invention such that the protein or portion thereof is able to participate in the maintenance of homeostasis in C. glutamicum, or to perform a function involved in the adaptation of this microorganism to different environmental conditions. Proteins involved in C. glutamicum cell wall biosynthesis or rearrangements, metabolism of -36inorganic compounds, modification or degradation of aromatic or aliphatic compounds, Sor that have a C. glutamicum enzymatic or proteolytic activity, as described herein, may play a role in the production and secretion of one or more fine chemicals. Examples of such activities are also described herein. Thus, "the function of an HA protein" contributes either directly or indirectly to the yield, production, and/or efficiency of production of one or more fine chemicals. Examples of HA protein activities are set 00 forth in Table 1.

In another embodiment, the protein is at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, 90-95%, and most C 10 preferably at least about 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing).

Portions of proteins encoded by the HA nucleic acid molecules of the invention are preferably biologically active portions of one of the HA proteins. As used herein, the term "biologically active portion of an HA protein" is intended to include a portion, a domain/motif, of an HA protein that can participate in the maintenance of homeostasis in C glutamicum, or that can perform a function involved in the adaptation of this microorganism to different environmental conditions, or has an activity as set forth in Table 1. To determine whether an HA protein or a biologically active portion thereof can participate in C. glutamicum cell wall biosynthesis or rearrangements, metabolism of inorganic compounds, modification or degradation of aromatic or aliphatic compounds, or has a C. glutamicum enzymatic or proteolytic activity, an assay of enzymatic activity may be performed. Such assay methods are well known to those of ordinary skill in the art, as detailed in Example 8 of the Exemplification.

Additional nucleic acid fragments encoding biologically active portions of an HA protein can be prepared by isolating a portion of one of the s amino acid sequences in of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing), expressing the encoded portion of the HA protein or peptide by recombinant expression in vitro) and assessing the activity of the encoded portion of the HA protein or peptide.

The invention further encompasses nucleic acid molecules that differ from one of the nucleotide sequences of the invention a sequence of an odd-numbered SEQ ID -37- (NI NO: of the Sequence Listing) (and portions thereof) due to degeneracy of the genetic Scode and thus encode the same HA protein as that encoded by the nucleotide sequences shown in of the invention. In another embodiment, an isolated nucleic acid molecule of the invention has a nucleotide sequence encoding a protein having an amino acid sequence shown in the Sequence Listing an even-numbered SEQ ID In a still further embodiment, the nucleic acid molecule of the invention encodes a full length 00 C. glutamicum protein which is substantially homologous to an amino acid sequence of r the invention (encoded by an open reading frame shown in an odd-numbered SEQ ID NO: of the Sequence Listing).

S 10 It will be understood by one of ordinary skill in the art that in one embodiment the sequences of the invention are not meant to include the sequences of the prior art, such as those Genbank sequences set forth in Tables 2 or 4 which were available prior to the present invention. In one embodiment, the invention includes nucleotide and amino acid sequences having a percent identity to a nucleotide or amino acid sequence of the invention which is greater than that of a sequence of the prior art a Genbank sequence (or the protein encoded by such a sequence) set forth in Tables 2 or For example, the invention includes a nucleotide sequence which is greater than and/or at least 39% identical to the nucleotide sequence designated RXA00471 (SEQ ID NO:293), a nucleotide sequence which is greater than and/or at least 41% identical to the nucleotide sequence designated RXA00500 (SEQ ID NO:143), and a nucleotide sequence which is greater than and/or at least 35% identical to the nucleotide sequence designated RXA00502(SEQ ID NO:147). One of ordinary skill in the art would be able to calculate the lower threshold of percent identity for any given sequence of the invention by examining the GAP-calculated percent identity scores set forth in Table 4 for each of the three top hits for the given sequence, and by subtracting the highest GAP-calculated percent identity from 100 percent. One of ordinary skill in the art will also appreciate that nucleic acid and amino acid sequences having percent identities greater than the lower threshold so calculated at least 50%, 51%, 52%, 53%, 54%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, -38- N, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 96%, 97%, 98%, 99% or more identical) are also encompassed by the invention.

In addition to the C. glutamicum HA nucleotide set forth in the Sequence Listing as odd-numbered SEQ ID NOs, it will be appreciated by those of ordinary skill in the art that DNA sequence polymorphisms that lead to changes in the amino acid sequences of HA proteins may exist within a population the C. glutamicum population). Such 00 0genetic polymorphism in the HA gene may exist among individuals within a population due to natural variation. As used herein, the terms "gene" and "recombinant gene" refer to nucleic acid molecules comprising an open reading frame encoding an HA protein, 10 preferably a C. glutamicum HA protein. Such natural variations can typically result in variance in the nucleotide sequence of the HA gene. Any and all such nucleotide variations and resulting amino acid polymorphisms in HA that are the result of natural variation and that do not alter the functional activity of HA proteins are intended to be within the scope of the invention.

Nucleic acid molecules corresponding to natural variants and non-C. glutamicum homologues of the C. glulamicum HA DNA of the invention can be isolated based on their homology to the C. glutamicum HA nucleic acid disclosed herein using the C.

glutamicum DNA, or a portion thereof, as a hybridization probe according to standard hybridization techniques under stringent hybridization conditions. Accordingly, in another embodiment, an isolated nucleic acid molecule of the invention is at least nucleotides in length and hybridizes under stringent conditions to the nucleic acid molecule comprising a nucleotide sequence of an odd-numbered SEQ 1D NO: of the Sequence Listing. In other embodiments, the nucleic acid is at least 30, 50, 100, 250 or more nucleotides in length. As used herein, the term "hybridizes under stringent conditions" is intended to describe conditions for hybridization and washing under which nucleotide sequences at least 60% homologous to each other typically remain hybridized to each other. Preferably, the conditions are such that sequences at least about 65%, more preferably at least about 70%, and even more preferably at least about or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to those of ordinary skill in the art and can be found in Current Protocols in Molecular Biology, John Wiley Sons, N.Y. (1989), 6.3.1-6.3.6.

A preferred, non-limiting example of stringent hybridization conditions are -39r, hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45 0 C, followed by Sone or more washes in 0.2 X SSC, 0.1% SDS at 50-65 0 C. Preferably, an isolated nucleic acid molecule of the invention that hybridizes under stringent conditions to a nucleotide sequence of the invention corresponds to a naturally-occurring nucleic acid molecule. As used herein, a "naturally-occurring" nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature 00 encodes a natural protein). In one embodiment, the nucleic acid encodes a natural C.

glutamicum HA protein.

In addition to naturally-occurring variants of the HA sequence that may exist in the population, one of ordinary skill in the art will further appreciate that changes can be introduced by mutation into a nucleotide sequence of the invention, thereby leading to changes in the amino acid sequence of the encoded HA protein, without altering the functional ability of the HA protein. For example, nucleotide substitutions leading to amino acid substitutions at "non-essential" amino acid residues can be made in a nucleotide sequence of the invention. A "non-essential" amino acid residue is a residue that can be altered from the wild-type sequence of one of the HA proteins an evennumbered SEQ ID NO: of the Sequence Listing) without altering the activity of said HA protein, whereas an "essential" amino acid residue is required for HA protein activity.

Other amino acid residues, however, those that are not conserved or only semiconserved in the domain having HA activity) may not be essential for activity and thus are likely to be amenable to alteration without altering HA activity.

Accordingly, another aspect of the invention pertains to nucleic acid molecules encoding HA proteins that contain changes in amino acid residues that are not essential for HA activity. Such HA proteins differ in amino acid sequence from a sequence of an even-numbered SEQ ID NO: of the Sequence Listing yet retain at least one of the HA activities described herein. In one embodiment, the isolated nucleic acid molecule comprises a nucleotide sequence encoding a protein, wherein the protein comprises an amino acid sequence at least about 50% homologous to an amino acid sequence of the invention and is capable of participating in the maintenance of homeostasis in C.

glutamicum, or of performing a function involved in the adaptation of this microorganism to different environmental conditions, or has one or more of the activities set forth in Table 1. Preferably, the protein encoded by the nucleic acid r, molecule is at least about 50-60% homologous to the amino acid sequence of one of the Sodd-numbered SEQ ID NOs of the Sequence Listing, more preferably at least about homologous to one of these sequences in, even more preferably at least about 80%, 80-90%, 90-95% homologous to one of these sequences, and most preferably at least about 96%, 97%, 98%, or 99% homologous to one of the amino acid sequences of the invention 00 To determine the percent homology of two amino acid sequences one of I the amino acid sequences of the invention and a mutant form thereof) or of two nucleic acids, the sequences are aligned for optimal comparison purposes gaps can be I 10 introduced in the sequence of one protein or nucleic acid for optimal alignment with the other protein or nucleic acid). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in one sequence one of the amino acid sequences of the invention) is occupied by the same amino acid residue or nucleotide as the corresponding position in the other sequence a mutant form of.the amino acid sequence), then the molecules are homologous at that position as used herein amino acid or nucleic acid "homology" is equivalent to amino acid or nucleic acid "identity"). The percent homology between the two sequences is a function of the number of identical positions shared by the sequences homology of identical positions/total of positions x 100).

An isolated nucleic acid molecule encoding an HA protein homologous to a protein sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) can be created by introducing one or more nucleotide substitutions, additions or deletions into a nucleotide sequence of the invention such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein. Mutations can be introduced into one of the nucleotide sequences of the invention by standard techniques, such as site-directed mutagenesis and PCRmediated mutagenesis. Preferably, conservative amino acid substitutions are made at one or more predicted non-essential amino acid residues. A "conservative amino acid substitution" is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains lysine, arginine, histidine), acidic side chains aspartic acid, glutamic -41- N acid), uncharged polar side chains glycine, asparagine, glutamine, serine, Sthreonine, tyrosine, cysteine), nonpolar side chains alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains

N

1 threonine, valine, isoleucine) and aromatic side chains tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue 0 in an HA protein is preferably replaced with another amino acid residue from the same 00 0side chain family. Alternatively, in another embodiment, mutations can be introduced C randomly along all or part of an HA coding sequence, such as by saturation 0mutagenesis, and the resultant mutants can be screened for an HA activity described C 10 herein to identify mutants that retain HA activity. Following mutagenesis of the nucleotide sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, the encoded protein can be expressed recombinantly and the activity of the protein can be determined using, for example, assays described herein (see Example 8 of the Exemplification).

In addition to the nucleic acid molecules encoding HA proteins described above, another aspect of the invention pertains to isolated nucleic acid molecules which are antisense thereto. An "antisense" nucleic acid comprises a nucleotide sequence which is complementary to a "sense" nucleic acid encoding a protein, complementary to the coding strand of a double-stranded DNA molecule or complementary to an mRNA sequence. Accordingly, an antisense nucleic acid can hydrogen bond to a sense nucleic acid. The antisense nucleic acid can be complementary to an entire HA coding strand, or to only a portion thereof. In one embodiment, an antisense nucleic acid molecule is antisense to a "coding region" of the coding strand of a nucleotide sequence encoding an HA protein. The term "coding region" refers to the region of the nucleotide sequence comprising codons which are translated into amino acid residues the entire coding region of SEQ ID NO. 3 (RXN00249) comprises nucleotides 1 to957). In another embodiment, the antisense nucleic acid molecule is antisense to a "noncoding region" of the coding strand of a nucleotide sequence encoding HA. The term "noncoding region" refers to 5' and 3' sequences which flank the coding region that are not translated into amino acids also referred to as 5' and 3' untranslated regions).

Given the coding strand sequences encoding HA disclosed herein the sequences set forth as odd-numbered SEQ ID NOs in the Sequence Listing), antisense -42r-nucleic acids of the invention can be designed according to the rules of Watson and Crick base pairing. The antisense nucleic acid molecule can be complementary to the entire coding region of HA mRNA, but more preferably is an oligonucleotide which is antisense to only a portion of the coding or noncoding region of HA mRNA. For example, the antisense oligonucleotide can be complementary to the region surrounding the translation start site of HA mRNA. An antisense oligonucleotide can be, for 00 example, about 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50 nucleotides in length. An ri antisense nucleic acid of the invention can be constructed using chemical synthesis and enzymatic ligation reactions using procedures known in the art. For example, an r 10 antisense nucleic acid an antisense oligonucleotide) can be chemically synthesized using naturally occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids, phosphorothioate derivatives and acridine substituted nucleotides can be used. Examples of modified nucleotides which can be used to generate the antisense nucleic acid include fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4acetylcytosine, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethyl-2thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-Dgalactosylqueosine, inosine, N6-isopentenyladenine, 1 -methylguanine, 1 -methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, acid wybutoxosine, pseudouracil, queosine, 2-thiocytosine, methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5- oxyacetic acid methylester, uracil-5-oxyacetic acid 5-methyl-2-thiouracil, 3-(3-amino-3-N-2carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine. Alternatively, the antisense nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).

-43l The antisense nucleic acid molecules of the invention are typically administered Sto a cell or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encoding an HA protein to thereby inhibit expression of the CN protein, by inhibiting transcription and/or translation. The hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule which binds to DNA duplexes, through 00 Sspecific interactions in the major groove of the double helix. The antisense molecule can Sbe modified such that it specifically binds to a receptor or an antigen expressed on a Sselected cell surface, by linking the antisense nucleic acid molecule to a peptide or an antibody which binds to a cell surface receptor or antigen. The antisense nucleic acid molecule can also be delivered to cells using the vectors described herein. To achieve sufficient intracellular concentrations of the antisense molecules, vector constructs in which the antisense nucleic acid molecule is placed under the control of a strong prokaryotic, viral, or eukaryotic promoter are preferred.

In yet another embodiment, the antisense nucleic acid molecule of the invention is an a-anomeric nucleic acid molecule. An a-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual P-units, the strands run parallel to each other (Gaultier et al. (1987) Nucleic Acids.

Res. 15:6625-6641). The antisense nucleic acid molecule can also comprise a 2'-omethylribonucleotide (Inoue et al. (1987) Nucleic Acids Res. 15:6131-6148) or a chimeric RNA-DNA analogue (Inoue et al. (1987) FEBS Lett. 215:327-330).

In still another embodiment, an antisense nucleic acid of the invention is a ribozyme. Ribozymes are catalytic RNA molecules with ribonuclease activity which are capable of cleaving a single-stranded nucleic acid, such as an mRNA, to which they have a complementary region. Thus, ribozymes hammerhead ribozymes (described in Haselhoff and Gerlach (1988) Nature 334:585-591)) can be used to catalytically cleave HA mRNA transcripts to thereby inhibit translation of HA mRNA.

A ribozyme having specificity for an HA-encoding nucleic acid can be designed based upon the nucleotide sequence of an HA DNA molecule disclosed herein SEQ ID NO. 3 (RXN00249)). For example, a derivative of a Tetrahymena L-19 IVS RNA can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in an HA-encoding mRNA. See, Cech et al.

-44- (N U.S. Patent No. 4,987,071 and Cech et al. U.S. Patent No. 5,116,742. Alternatively, HA mRNA can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules. See, Bartel, D. and Szostak, J.W. (1993) Science 261:1411-1418.

Alternatively, HA gene expression can be inhibited by targeting nucleotide 0 sequences complementary to the regulatory region of an HA nucleotide sequence 00 0 an HA promoter and/or enhancers) to form triple helical structures that prevent 0 CN transcription of an HA gene in target cells. See generally, Helene, C. (1991) Anticancer 0 Drug Des. 6(6):569-84; Helene, C. et al. (1992) Ann. N.Y. Acad. Sci. 660:27-36; and C 10 Maher, L.J. (1992) Bioassays 14(12):807-15.

B. Recombinant Expression Vectors and Host Cells Another aspect of the invention pertains to vectors, preferably expression vectors, containing a nucleic acid encoding an HA protein (or a portion thereof). As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a "plasmid", which refers to a circular double stranded DNA loop into which additional DNA segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as "expression vectors". In general, expression vectors of utility in recombinant DNA techniques are often in the form ofplasmids. In the present specification, "plasmid" and "vector" can be used interchangeably as the plasmid is the most commonly used form of vector.

However, the invention is intended to include such other forms of expression vectors, such as viral vectors replication defective retroviruses, adenoviruses and adenoassociated viruses), which serve equivalent functions.

C

N The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory N sequences, selected on the basis of the host cells to be used for expression, which are operatively linked to the nucleic acid sequence to be expressed. Within a recombinant 0 expression vector, "operably linked" is intended to mean that the nucleotide sequence of 00 0interest is linked to the regulatory sequence(s) in a manner which allows for expression C of the nucleotide sequence in an in vitro transcription/translation system or in a \0 0host cell when the vector is introduced into the host cell). The term "regulatory C 10 sequence" is intended to include promoters, enhancers and other expression control elements polyadenylation signals). Such regulatory sequences are described, for example, in Goeddel; Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Regulatory sequences include those which direct constitutive expression of a nucleotide sequence in many types of host cell and those which direct expression of the nucleotide sequence only in certain host cells.

Preferred regulatory sequences are, for example, promoters such as cos-, tac-, trp-, tet-, trp-tet-, Ipp-, lac-, Ipp-lac-, lacI"-, T7-, T5-, T3-, gal-, trc-, ara-, SP6-, amy, SP02, X-PRor X PL, which are used preferably in bacteria. Additional regulatory sequences are, for example, promoters from yeasts and fungi, such as ADC 1, MFa, AC, P-60, CYC 1, GAPDH, TEF, rp28, ADH, promoters from plants such as CaMV/35S, SSU, OCS, lib4, usp, STLSI, B33, nos or ubiquitin- or phaseolin-promoters. It is also possible to use artificial promoters. It will be appreciated by those of ordinary skill in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein HA proteins, mutant forms of HA proteins, fusion proteins, etc.).

The recombinant expression vectors of the invention can be designed for expression of HA proteins in prokaryotic or eukaryotic cells. For example, HA genes can be expressed in bacterial cells such as C. glutamicum, insect cells (using baculovirus expression vectors), yeast and other fungal cells (see Romanos, M.A. et al. (1992) "Foreign gene expression in yeast: a review", Yeast 8: 423-488; van den Hondel, -46- C.A.M.J.J. et al. (1991) "Heterologous gene expression in filamentous fungi" in: More SGene Manipulations in Fungi, J.W. Bennet L.L. Lasure, eds., p. 396-428: Academic Press: San Diego; and van den Hondel, C.A.M.J.J. Punt, P.J. (1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, Peberdy, J.F. et al., eds., p. 1-28, Cambridge University Press: Cambridge), 0 algae and multicellular plant cells (see Schmidt, R. and Willmitzer, L. (1988) High 00 Sefficiency Agrobacterium tumefaciens -mediated transformation ofArabidopsis thaliana leaf and cotyledon explants" Plant Cell Rep.: 583-586), or mammalian cells.

SSuitable host cells are discussed further in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.

Expression of proteins in prokaryotes is most often carried out with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion proteins. Fusion vectors add a number of amino acids to a protein encoded therein, usually to the amino terminus of the recombinant protein but also to the C-terminus or fused within suitable regions in the proteins. Such fusion vectors typically serve three purposes: 1) to increase expression of recombinant protein; 2) to increase the solubility of the recombinant protein; and 3) to aid in the purification of the recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant protein to enable separation of the recombinant protein from the fusion moiety subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase.

Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith, D.B. and Johnson, K.S. (1988) Gene 67:31-40), pMAL (New England Biolabs, Beverly, MA) and pRIT5 (Pharmacia, Piscataway, NJ) which fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. In one embodiment, the coding sequence of the HA protein is cloned into a pGEX expression vector to create a vector encoding a fusion protein comprising, from the N-terminus to the C-terminus, GST-thrombin cleavage site-X protein. The fusion protein can be purified by affinity chromatography using glutathione-agarose resin.

-47- CN Recombinant HA protein unfused to GST can be recovered by cleavage of the fusion Sprotein with thrombin.

Examples of suitable inducible non-fusion E. coli expression vectors include C1 pTrc (Amann et al., (1988) Gene 69:301-315) pLG338, pACYC184, pBR322, pUC18, pUC19, pKC30, pRep4, pHS1, pHS2, pPLc236, pMBL24, pLG200, pUR290, pIN- 0 III113-B1, Xgt 11, pBdCl, and pET 1 d (Studier et al., Gene Expression Technology: 00 0 Methods in Enzymology 185, Academic Press, San Diego, California (1990) 60-89; and C Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

(N

STarget gene expression from the pTrc vector relies on host RNA polymerase C 10 transcription from a hybrid trp-lac fusion promoter. Target gene expression from the pET 1 ld vector relies on transcription from a T7 gn 0-lac fusion promoter mediated by a coexpressed viral RNA polymerase (T7 gnl). This viral polymerase is supplied by host strains BL21(DE3) or HMS174(DE3) from a resident X prophage harboring a T7 gnl gene under the transcriptional control of the lacUV 5 promoter. For transformation of other varieties of bacteria, appropriate vectors may be selected. For example, the plasmids pIJ1 01, pIJ364, pIJ702 and pIJ361 are known to be useful in transforming Streptomyces, while plasmids pUB 110, pC 194, or pBD214 are suited for transformation of Bacillus species. Several plasmids of use in the transfer of genetic information into Corynebacterium include pHM1519, pBL1, pSA77, or pAJ667 (Pouwels et al., eds.

(1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

One strategy to maximize recombinant protein expression is to express the protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein (Gottesman, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 119-128). Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in the bacterium chosen for expression, such as C. glutamicum (Wada et al. (1992) Nucleic Acids Res. 20: 2111-2118). Such alteration of nucleic acid sequences of the invention can be carried out by standard DNA synthesis techniques.

In another embodiment, the HA protein expression vector is a yeast expression vector. Examples of vectors for expression in yeast S. cerevisiae include pYepSecl (Baldari, et al., (1987) Embo J. 6:229-234), 2 t, pAG-1, Yep6, Yepl3, pEMBLYe23, -48- (1I pMFa (Kurjan and Herskowitz, (1982) Cell 30:933-943), pJRY88 (Schultz et al., (1987) dGene 54:113-123), and pYES2 (Invitrogen Corporation, San Diego, CA). Vectors and methods for the construction of vectors appropriate for use in other fungi, such as the r1 filamentous fungi, include those detailed in: van den Hondel, C.A.M.J.J. Punt, P.J.

(1991) "Gene transfer systems and vector development for filamentous fungi, in: 0 Applied Molecular Genetics of Fungi, J.F. Peberdy, et al., eds., p. 1-28, Cambridge 00 SUniversity Press: Cambridge, and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: C New York (IBSN 0 444 904018).

0 Alternatively, the HA proteins of the invention can be expressed in insect cells C 10 using baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells Sf9 cells) include the pAc series (Smith et al.

(1983) Mol. Cell Biol. 3:2156-2165) and the pVL series (Lucklow and Summers (1989) Virology 170:31-39).

In another embodiment, the HA proteins of the invention may be expressed in unicellular plant cells (such as algae) or in plant cells from higher plants the spermatophytes, such as crop plants). Examples of plant expression vectors include those detailed in: Becker, Kemper, Schell, J. and Masterson, R. (1992) "New plant binary vectors with selectable markers located proximal to the left border", Plant Mol. Biol. 20: 1195-1197; and Bevan, M.W. (1984) "Binary Agrobacterium vectors for plant transformation", Nucl. Acid. Res. 12: 8711-8721, and include pLGV23, pGHlac+, pBIN19, pAK2004, and pDH51 (Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

In yet another embodiment, a nucleic acid of the invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include pCDM8 (Seed, B. (1987) Nature 329:840) and pMT2PC (Kaufman et al. (1987) EMBOJ. 6:187-195). When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements.

For example, commonly used promoters are derived from polyoma, Adenovirus 2, cytomegalovirus and Simian Virus 40. For other suitable expression systems for both prokaryotic and eukaryotic cells see chapters 16 and 17 of Sambrook, Fritsh, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring -49- CN Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, S1989.

In another embodiment, the recombinant mammalian expression vector is C1 capable of directing expression of the nucleic acid preferentially in a particular cell type tissue-specific regulatory elements are used to express the nucleic acid). Tissuespecific regulatory elements are known in the art. Non-limiting examples of suitable 00 O tissue-specific promoters include the albumin promoter (liver-specific; Pinkert et al.

S(1987) Genes Dev. 1:268-277), lymphoid-specific promoters (Calame and Eaton (1988) SAdv. Immunol. 43:235-275), in particular promoters of T cell receptors (Winoto and Baltimore (1989) EMBO J. 8:729-733) and immunoglobulins (Banerji et al. (1983) Cell 33:729-740; Queen and Baltimore (1983) Cell 33:741-748), neuron-specific promoters the neurofilament promoter; Byrne and Ruddle (1989) PNAS 86:5473-5477), pancreas-specific promoters (Edlund et al. (1985) Science 230:912-916), and mammary gland-specific promoters milk whey promoter; U.S. Patent No. 4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are also encompassed, for example the murine hox promoters (Kessel and Gruss (1990) Science 249:374-379) and the ac-fetoprotein promoter (Campes and Tilghman (1989) Genes Dev. 3:537-546).

The invention further provides a recombinant expression vector comprising a DNA molecule of the invention cloned into the expression vector in an antisense orientation. That is, the DNA molecule is operatively linked to a regulatory sequence in a manner which allows for expression (by transcription of the DNA molecule) of an RNA molecule which is antisense to HA mRNA. Regulatory sequences operatively linked to a nucleic acid cloned in the antisense orientation can be chosen which direct the continuous expression of the antisense RNA molecule in a variety of cell types, for instance viral promoters and/or enhancers, or regulatory sequences can be chosen which direct constitutive, tissue specific or cell type specific expression of antisense RNA.

The antisense expression vector can be in the form of a recombinant plasmid, phagemid or attenuated virus in which antisense nucleic acids are produced under the control of a high efficiency regulatory region, the activity of which can be determined by the cell type into which the vector is introduced. For a discussion of the regulation of gene r, expression using antisense genes see Weintraub, H. et al., Antisense RNA as a Smolecular tool for genetic analysis, Reviews Trends in Genetics, Vol. 1(1) (1986).

Another aspect of the invention pertains to host cells into which a recombinant

C

expression vector of the invention has been introduced. The terms "host cell" and "recombinant host cell" are used interchangeably herein. It is understood that such 0 terms refer not only to the particular subject cell but to the progeny or potential progeny 00 0of such a cell. Because certain modifications may occur in succeeding generations due C to either mutation or environmental influences, such progeny may not, in fact, be 0identical to the parent cell, but are still included within the scope of the term as used herein.

A host cell can be any prokaryotic or eukaryotic cell. For example, an HA protein can be expressed in bacterial cells such as C. glutamicum, insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known to those of ordinary skill in the art. Microorganisms related to Corynebacterium glutamicum which may be conveniently used as host cells for the nucleic acid and protein molecules of the invention are set forth in Table 3.

Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms "transformation" and "transfection", "conjugation" and "transduction" are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid linear DNA or RNA a linearized vector or a gene construct alone without a vector) or nucleic acid in the form of a vector a plasmid, phage, phasmid, phagemid, transposon or other DNA) into a host cell, including calcium phosphate or calcium chloride co-precipitation, DEAE-dextran-mediated transfection, lipofection, natural competence, chemical-mediated transfer, or electroporation. Suitable methods for transforming or transfecting host cells can be found in Sambrook, et al. (Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989), and other laboratory manuals.

For stable transfection of mammalian cells, it is known that, depending upon the expression vector and transfection technique used, only a small fraction of cells may integrate the foreign DNA into their genome. In order to identify and select these integrants, a gene that encodes a selectable marker resistance to antibiotics) is -51- (1N generally introduced into the host cells along with the gene of interest. Preferred selectable markers include those which confer resistance to drugs, such as G418, hygromycin and methotrexate. Nucleic acid encoding a selectable marker can be C introduced into a host cell on the same vector as that encoding an HA protein or can be introduced on a separate vector. Cells stably transfected with the introduced nucleic acid can be identified by, for example, drug selection cells that have incorporated 00 0the selectable marker gene will survive, while the other cells die).

To create a homologous recombinant microorganism, a vector is prepared which contains at least a portion of an HA gene into which a deletion, addition or substitution ,1 0 has been introduced to thereby alter, functionally disrupt, the HA gene. Preferably, this HA gene is a Corynebacterium glutamicum HA gene, but it can be a homologue from a related bacterium or even from a mammalian, yeast, or insect source. In a preferred embodiment, the vector is designed such that, upon homologous recombination, the endogenous HA gene is functionally disrupted no longer encodes a functional protein; also referred to as a "knock out" vector). Alternatively, the vector can be designed such that, upon homologous recombination, the endogenous HA gene is mutated or otherwise altered but still encodes functional protein the upstream regulatory region can be altered to thereby alter the expression of the endogenous HA protein). In the homologous recombination vector, the altered portion of the HA gene is flanked at its 5' and 3' ends by additional nucleic acid of the HA gene to allow for homologous recombination to occur between the exogenous HA gene carried by the vector and an endogenous HA gene in a microorganism. The additional flanking HA nucleic acid is of sufficient length for successful homologous recombination with the endogenous gene. Typically, several kilobases of flanking DNA (both at the 5' and 3' ends) are included in the vector (see Thomas, and Capecchi, M.R. (1987) Cell 51: 503 for a description of homologous recombination vectors). The vector is introduced into a microorganism by electroporation) and cells in which the introduced HA gene has homologously recombined with the endogenous HA gene are selected, using art-known techniques.

In another embodiment, recombinant microorganisms can be produced which contain selected systems which allow for regulated expression of the introduced gene.

For example, inclusion of an HA gene on a vector placing it under control of the lac -52- (1N operon permits expression of the HA gene only in the presence of IPTG. Such regulatory systems are well known in the art.

In another embodiment, an endogenous HA gene in a host cell is disrupted by homologous recombination or other genetic means known in the art) such that expression of its protein product does not occur. In another embodiment, an endogenous or introduced HA gene in a host cell has been altered by one or more point mutations, 00 deletions, or inversions, but still encodes a functional HA protein. In still another C, embodiment, one or more of the regulatory regions a promoter, repressor, or inducer) of an HA gene in a microorganism has been altered by deletion, 10 truncation, inversion, or point mutation) such that the expression of the HA gene is modulated. One of ordinary skill in the art will appreciate that host cells containing more than one of the described HA gene and protein modifications may be readily produced using the methods of the invention, and are meant to be included in the present invention.

A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce express) an HA protein. Accordingly, the invention further provides methods for producing HA proteins using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding an HA protein has been introduced, or into which genome has been introduced a gene encoding a wild-type or altered HA protein) in a suitable medium until HA protein is produced. In another embodiment, the method further comprises isolating HA proteins from the medium or the host cell.

C. Isolated HA Proteins Another aspect of the invention pertains to isolated HA proteins, and biologically active portions thereof. An "isolated" or "purified" protein or biologically active portion thereof is substantially free of cellular material when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized.

The language "substantially free of cellular material" includes preparations of HA protein in which the protein is separated from cellular components of the cells in which it is naturally or recombinantly produced. In one embodiment, the language -53- (1,I "substantially free of cellular material" includes preparations of HA protein having less Sthan about 30% (by dry weight) of non-HA protein (also referred to herein as a "contaminating protein"), more preferably less than about 20% of non-HA protein, still C more preferably less than about 10% of non-HA protein, and most preferably less than about 5% non-HA protein. When the HA protein or biologically active portion thereof N is recombinantly produced, it is also preferably substantially free of culture medium, i.e., 00 0culture medium represents less than about 20%, more preferably less than about and most preferably less than about 5% of the volume of the protein preparation. The Ilanguage "substantially free of chemical precursors or other chemicals" includes 10 preparations of HA protein in which the protein is separated from chemical precursors or other chemicals which are involved in the synthesis of the protein. In one embodiment, the language "substantially free of chemical precursors or other chemicals" includes preparations of HA protein having less than about 30% (by dry weight) of chemical precursors or non-HA chemicals, more preferably less than about 20% chemical precursors or non-HA chemicals, still more preferably less than about 10% chemical precursors or non-HA chemicals, and most preferably less than about 5% chemical precursors or non-HA chemicals. In preferred embodiments, isolated proteins or biologically active portions thereof lack contaminating proteins from the same organism from which the HA protein is derived. Typically, such proteins are produced by recombinant expression of, for example, a C. glutamicum HA protein in a microorganism such as C. glutamicum.

An isolated HA protein or a portion thereof of the invention can participate in the repair or recombination of DNA, in the transposition of genetic material, in gene expression the processes of transcription or translation), in protein folding, or in protein secretion in Corynebacterium glutamicum, or has one or more of the activities set forth in Table 1. In preferred embodiments, the protein or portion thereof comprises an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) such that the protein or portion thereof maintains the ability to participate in the maintenance of homeostasis in C. glutamicum, or to perform a function involved in the adaptation of this microorganism to different environmental conditions. The portion of the protein is preferably a biologically active portion as described herein. In another -54- (1,I preferred embodiment, an HA protein of the invention has an amino acid sequence set Sforth as an even-numbered SEQ ID NO: of the Sequence Listing. In yet another preferred embodiment, the HA protein has an amino acid sequence which is encoded by i a nucleotide sequence which hybridizes, hybridizes under stringent conditions, to a nucleotide sequence the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing). In still another preferred embodiment, the HA protein has an 00 amino acid sequence which is encoded by a nucleotide sequence that is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at 10 least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to one of the nucleic acid sequences of the invention, or a portion thereof. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. The preferred HA proteins of the present invention also preferably possess at least one of the HA activities described herein. For example, a preferred HA protein of the present invention includes an amino acid sequence encoded by a nucleotide sequence which hybridizes, e.g., hybridizes under stringent conditions, to a nucleotide sequence of the invention, and which can participate in the maintenance of homeostasis in C. glutamicum, or can perform a function involved in the adaptation of this microorganism to different environmental conditions, or which has one or more of the activities set forth in Table 1.

In other embodiments, the HA protein is substantially homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) and retains the functional activity of the protein of one of the amino acid sequences of the invention yet differs in amino acid sequence due to natural variation or mutagenesis, as described in detail in subsection I above. Accordingly, in another embodiment, the HA protein is a protein which comprises an amino acid sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, r, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of the invention and which has at least one of the HA activities described herein. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 80-95% identical) 00 oO are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or Slower limits are intended to be included. In another embodiment, the invention pertains 10 to a full length C. glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention.

Biologically active portions of an HA protein include peptides comprising amino acid sequences derived from the amino acid sequence of an HA protein, the amino acid sequence of an even-numbered SEQ ID NO: of the Sequence Listing, the amino acid sequence of a protein homologous to an HA protein, which include fewer amino acids than a full length HA protein or the full length protein which is homologous to an HA protein, and exhibit at least one activity of an HA protein. Typically, biologically active portions (peptides, peptides which are, for example, 5, 10, 15, 20, 30, 35, 36, 37, 38, 39, 40, 50, 100 or more amino acids in length) comprise a domain or motif with at least one activity of an HA protein. Moreover, other biologically active portions, in which other regions of the protein are deleted, can be prepared by recombinant techniques and evaluated for one or more of the activities described herein. Preferably, the biologically active portions of an HA protein include one or more selected domains/motifs or portions thereof having biological activity.

HA proteins are preferably produced by recombinant DNA techniques. For example, a nucleic acid molecule encoding the protein is cloned into an expression vector (as described above), the expression vector is introduced into a host cell (as described above) and the HA protein is expressed in the host cell. The HA protein can then be isolated from the cells by an appropriate purification scheme using standard protein purification techniques. Alternative to recombinant expression, an HA protein, polypeptide, or peptide can be synthesized chemically using standard peptide synthesis techniques. Moreover, native HA protein can be isolated from cells endothelial -56- N cells), for example using an anti-HA antibody, which can be produced by standard techniques utilizing an HA protein or fragment thereof of this invention.

The invention also provides HA chimeric or fusion proteins. As used herein, an C HA "chimeric protein" or "fusion protein" comprises an HA polypeptide operatively linked to a non-HA polypeptide. An "HA polypeptide" refers to a polypeptide having an Samino acid sequence corresponding to an HA protein, whereas a "non-HA polypeptide" 0 00 refers to a polypeptide having an amino acid sequence corresponding to a protein which Sis not substantially homologous to the HA protein, a protein which is different from the HA protein and which is derived from the same or a different organism. Within the fusion protein, the term "operatively linked" is intended to indicate that the HA polypeptide and the non-HA polypeptide are fused in-frame to each other. The non-HA polypeptide can be fused to the N-terminus or C-terminus of the HA polypeptide. For example, in one embodiment the fusion protein is a GST-HA fusion protein in which the HA sequences are fused to the C-terminus of the GST sequences. Such fusion proteins can facilitate the purification of recombinant HA proteins. In another embodiment, the fusion protein is an HA protein containing a heterologous signal sequence at its Nterminus. In certain host cells mammalian host cells), expression and/or secretion of an HA protein can be increased through use of a heterologous signal sequence.

Preferably, an HA chimeric or fusion protein of the invention is produced by standard recombinant DNA techniques. For example, DNA fragments coding for the different polypeptide sequences are ligated together in-frame in accordance with conventional techniques, for example by employing blunt-ended or stagger-ended termini for ligation, restriction enzyme digestion to provide for appropriate termini, filling-in of cohesive ends as appropriate, alkaline phosphatase treatment to avoid undesirable joining, and enzymatic ligation. In another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers.

Alternatively, PCR amplification of gene fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and reamplified to generate a chimeric gene sequence (see, for example, Current Protocols in Molecular Biology, eds. Ausubel et al. John Wiley Sons: 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety a GST polypeptide). An HA- -57- CN encoding nucleic acid can be cloned into such an expression vector such that the fusion Smoiety is linked in-frame to the HA protein.

Homologues of the HA protein can be generated by mutagenesis, discrete CN point mutation or truncation of the HA protein. As used herein, the term "homologue" refers to a variant form of the HA protein which acts as an agonist or antagonist of the activity of the HA protein. An agonist of the HA protein can retain substantially the Ssame, or a subset, of the biological activities of the HA protein. An antagonist of the HA protein can inhibit one or more of the activities of the naturally occurring form of Sthe HA protein, by, for example, competitively binding to a downstream or upstream 10 member of a biochemical cascade which includes the HA protein, by binding to a target molecule with which the HA protein interacts, such that no functional interaction is possible, or by binding directly to the HA protein and inhibiting its normal activity.

In an alternative embodiment, homologues of the HA protein can be identified by screening combinatorial libraries of mutants, truncation mutants, of the HA protein for HA protein agonist or antagonist activity. In one embodiment, a variegated library of HA variants is generated by combinatorial mutagenesis at the nucleic acid level and is encoded by a variegated gene library. A variegated library of HA variants can be produced by, for example, enzymatically ligating a mixture of synthetic oligonucleotides into gene sequences such that a degenerate set of potential HA sequences is expressible as individual polypeptides, or alternatively, as a set of larger fusion proteins for phage display) containing the set of HA sequences therein.

There are a variety of methods which can be used to produce libraries of potential HA homologues from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA synthesizer, and the synthetic gene then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, in one mixture, of all of the sequences encoding the desired set of potential HA sequences. Methods for synthesizing degenerate oligonucleotides are known in the art (see, Narang, S.A. (1983) Tetrahedron 39:3; Itakura et al. (1984) Annu. Rev. Biochem. 53:323; Itakura et al. (1984) Science 198:1056; Ike et al. (1983) Nucleic Acid Res. 11:477.

In addition, libraries of fragments of the HA protein coding can be used to generate a variegated population of HA fragments for screening and subsequent -58- C1 selection of homologues of an HA protein. In one embodiment, a library of coding Qsequence fragments can be generated by treating a double stranded PCR fragment of an HA coding sequence with a nuclease under conditions wherein nicking occurs only C1, about once per molecule, denaturing the double stranded DNA, renaturing the DNA to form double stranded DNA which can include sense/antisense pairs from different nicked products, removing single stranded portions from reformed duplexes by 0 0treatment with S1 nuclease, and ligating the resulting fragment library into an expression vector. By this method, an expression library can be derived which encodes N-terminal, C-terminal and internal fragments of various sizes of the HA protein.

C 10 Several techniques are known in the art for screening gene products of combinatorial libraries made by point mutations or truncation, and for screening cDNA libraries for gene products having a selected property. Such techniques are adaptable for rapid screening of the gene libraries generated by the combinatorial mutagenesis of HA homologues. The most widely used techniques, which are amenable to high through-put analysis, for screening large gene libraries typically include cloning the gene library into replicable expression vectors, transforming appropriate cells with the resulting library of vectors, and expressing the combinatorial genes under conditions in which detection of a desired activity facilitates isolation of the vector encoding the gene whose product was detected. Recursive ensemble mutagenesis (REM), a new technique which enhances the frequency of functional mutants in the libraries, can be used in combination with the screening assays to identify HA homologues (Arkin and Yourvan (1992) PNAS 89:7811-7815; Delgrave et al. (1993) Protein Engineering 6(3):327-331).

In another embodiment, cell based assays can be exploited to analyze a variegated HA library, using methods well known in the art.

D. Uses and Methods of the Invention The nucleic acid molecules, proteins, protein homologues, fusion proteins, primers, vectors, and host cells described herein can be used in one or more of the following methods: identification of C. glutamicum and related organisms; mapping of genomes of organisms related to C. glutamicum; identification and localization of C.

glutamicum sequences of interest; evolutionary studies; determination of HA protein regions required for function; modulation of an HA protein activity; modulation of the -59- CN metabolism of one or more inorganic compounds; modulation of the modification or degradation of one or more aromatic or aliphatic compounds; modulation of cell wall synthesis or rearrangements; modulation of enzyme activity or proteolysis; and Ci modulation of cellular production of a desired compound, such as a fine chemical.

The HA nucleic acid molecules of the invention have a variety of uses. First, they may be used to identify an organism as being Corynebacterium glutamicum or a Sclose relative thereof. Also, they may be used to identify the presence of C. glutamicum or a relative thereof in a mixed population of microorganisms. The invention provides Sthe nucleic acid sequences of a number of C. glutamicum genes; by probing the extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a C. glutamicum gene which is unique to this organism, one can ascertain whether this organism is present.

Although Corynebacterium glutamicum itself is nonpathogenic, it is related to pathogenic species, such as Corynebacterium diphtheriae. Corynebacterium diphtheriae is the causative agent of diphtheria, a rapidly developing, acute, febrile infection which involves both local and systemic pathology. In this disease, a local lesion develops in the upper respiratory tract and involves necrotic injury to epithelial cells; the bacilli secrete toxin which is disseminated through this lesion to distal susceptible tissues of the body. Degenerative changes brought about by the inhibition of protein synthesis in these tissues, which include heart, muscle, peripheral nerves, adrenals, kidneys, liver and spleen, result in the systemic pathology of the disease. Diphtheria continues to have high incidence in many parts of the world, including Africa, Asia, Eastern Europe and the independent states of the former Soviet Union. An ongoing epidemic of diphtheria in the latter two regions has resulted in at least 5,000 deaths since 1990.

In one embodiment, the invention provides a method of identifying the presence or activity of Cornyebacterium diphtheriae in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention the sequences set forth in as odd-numbered or even-numbered SEQ ID NOs, respectively, in the Sequence Listing) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject. C. glutamicum and C diphtheriae are related bacteria, and many of the nucleic acid and protein molecules in C. glutamicum N are homologous to C. diphtheriae nucleic acid and protein molecules, and can therefore be used to detect C. diphtheriae in a subject.

The nucleic acid and protein molecules of the invention may also serve as Smarkers for specific regions of the genome. This has utility not only in the mapping of the genome, but also for functional studies of C. glutamicum proteins. For example, to identify the region of the genome to which a particular C glutamicum DNA-binding OO protein binds, the C. glutamnicumn genome could be digested, and the fragments incubated with the DNA-binding protein. Those which bind the protein may be additionally probed Nwith the nucleic acid molecules of the invention, preferably with readily detectable labels; binding of such a nucleic acid molecule to the genome fragment enables the localization of the fragment to the genome map of C. glutamicum, and, when performed multiple times with different enzymes, facilitates a rapid determination of the nucleic acid sequence to which the protein binds. Further, the nucleic acid molecules of the invention may be sufficiently homologous to the sequences of related species such that these nucleic acid molecules may serve as markers for the construction of a genomic map in related bacteria, such as Brevibacterium lactofermentum.

The HA nucleic acid molecules of the invention are also useful for evolutionary and protein structural studies. The processes involved in adaptation and the maintenance of homeostasis in which the molecules of the invention participate are utilized by a wide variety of species; by comparing the sequences of the nucleic acid molecules of the present invention to those encoding similar enzymes from other organisms, the evolutionary relatedness of the organisms can be assessed. Similarly, such a comparison permits an assessment of which regions of the sequence are conserved and which are not, which may aid in determining those regions of the protein which are essential for the functioning of the enzyme. This type of determination is of value for protein engineering studies and may give an indication of what the protein can tolerate in terms ofmutagenesis without losing function.

Manipulation of the HA nucleic acid molecules of the invention may result in the production of HA proteins having functional differences from the wild-type HA proteins. These proteins may be improved in efficiency or activity, may be present in greater numbers in the cell than is usual, or may be decreased in efficiency or activity.

-61c The invention provides methods for screening molecules which modulate the activity of an HA protein, either by interacting with the protein itself or a substrate or Sbinding partner of the HA protein, or by modulating the transcription or translation of an ci HA nucleic acid molecule of the invention. In such methods, a microorganism expressing one or more HA proteins of the invention is contacted with one or more test Ni compounds, and the effect of each test compound on the activity or level of expression 0O 00of the HA protein is assessed.

SThe modulation of activity or number of HA proteins involved in cell wall Ibiosynthesis or rearrangements may impact the production, yield, and/or efficiency of production of one or more fine chemicals from C. glutamicum cells. For example, by altering the activity of these proteins, it may be possible to modulate the structure or thickness of the cell wall. The cell wall serves in large measure as a protective device against osmotic lysis and external sources of injury; by modifying the cell wall it may be possible to increase the ability of C. glutamicum to withstand the mechanical and shear force stresses encountered by this microorganism during large-scale fermentor culture.

Further, each C. glutamicum cell is surrounded by a thick cell wall, and thus, a significant portion of the biomass present in large scale culture consists of cell wall. By increasing the rate at which the cell wall is synthesized or by activating cell wall synthesis (through genetic engineering of the HA cell wall proteins of the invention) it may be possible to improve the growth rate of the microorganism. Similarly, by decreasing the activity or number of proteins involved in the degradation of cell wall or by decreasing the repression of cell wall biosynthesis, an overall increase in cell wall production may be achieved. An increase in the number of viable C. glutamicum cells (as may be accomplished by any of the foregoing described protein alterations) should result in increased numbers of cells producing the desired fine chemical in large-scale fermentor culture, which should permit increased yields or efficiency of production of these compounds from the culture.

The modulation of activity or number of C. glutamicum HA proteins that participate in the modification or degradation of aromatic or aliphatic compounds may also have direct or indirect impacts on the production of one or more fine chemicals from these cells. Certain aromatic or aliphatic modification or degradation products are desirable fine chemicals organic acids or modified aromatic and aliphatic -62- N, compounds); thus, by modifying the enzymes which perform these modifications ,.hydroxylation, methylation, or isomerization) or degradation reactions, it may be possible to increase the yields of these desired compounds. Similarly, by decreasing the r, activity or number of proteins involved in pathways which further degrade the modified or breakdown products of the aforementioned reactions it may be possible to improve the yields of these fine chemicals from C. glutamicum cells in culture.

O

0These aromatic and aliphatic modification and degradative enzymes are themselves fine chemicals. In purified form, these enzymes may be used to degrade aromatic and aliphatic compounds toxic chemicals such as petroleum products), 10 either for the bioremediation of polluted sites, for the engineered decomposition of wastes, or for the large-scale and economically feasible production of desired modified aromatic or aliphatic compounds or their breakdown products, some of which may be conveniently used as carbon or energy sources for other fine chemical-producing compounds in culture (see, Faber, K. (1995) Biotransformations in Organic Chemistry, Springer: Berlin and references therein; and Roberts, ed. (1992-1996) Preparative Biotransformations, Wiley: Chichester, and references therein). By genetically altering these proteins such that their regulation by other cellular mechanisms is lessened or abolished, it may be possible to increase the overall number or activity of these proteins, thereby improving not only the yield of these fine chemicals but also the activity of these harvested proteins.

The modification of these aromatic and aliphatic modifying and degradation enzymes may also have an indirect effect on the production of one or more fine chemical. Many aromatic and aliphatic compounds (such as those that may be encountered as impurities in culture media or as waste products from cellular metabolism) are toxic to cells; by modifying and/or degrading these compounds such that they may be readily removed or destroyed, cellular viability should be increased.

Further, these enzymes may modify or degrade these compounds in such a manner that the resulting products may enter the normal carbon metabolism pathways of the cell, thus rendering the cell able to use these compounds as alternate carbon or energy sources. In large-scale culture situations, when there may be limiting amounts of optimal carbon sources, these enzymes provide a method by which cells may continue to grow and divide using aromatic or aliphatic compounds as nutrients. In either case, the -63 N, resulting increase in the number ofC. glutamicum cells in the culture producing the Sdesired fine chemical should in turn result in increased yields or efficiency of production of the fine chemical(s).

I Modifications in activity or number of HA proteins involved in the metabolism of inorganic compounds may also directly or indirectly affect the production of one or more fine chemicals from C. glutamicum or related bacterial cultures. For example, 00 0O many desirable fine chemicals, such as nucleic acids, amino acids, cofactors and vitamins thiamine, biotin, and lipoic acid) cannot be synthesized without inorganic ID molecules such as phosphorous, nitrate, sulfate, and iron. The inorganic metabolism proteins of the invention permit the cell to obtain these molecules from a variety of inorganic compounds and to divert them into various fine chemical biosynthetic pathways. Therefore, by increasing the activity or number of enzymes involved in the metabolism of these inorganic compounds, it may be possible to increase the supply of these possibly limiting inorganic molecules, thereby directly increasing the production or efficiency of production of various fine chemicals from C. glutamicum cells containing such altered proteins. Modification of the activity or number of inorganic metabolism enzymes of the invention may also render C. glutamicum able to better utilize limited inorganic compound supplies, or to utilize nonoptimal inorganic compounds to synthesize amino acids, vitamins, cofactors, or nucleic acids, all of which are necessary for continued growth and replication of the cell. By improving the viability of these cells in large-scale culture, the number of C. glutamicum cells producing one or more fine chemicals in the culture may also be increased, in turn increasing the yields or efficiency of production of one or more fine chemicals.

C. glutamicum enzymes for general processes are themselves desirable fine chemicals. The specific properties of enzymes regio- and stereospecificity, among others) make them useful catalysts for chemical reactions in vitro. Either whole C.

glutamicum cells may be incubated with an appropriate substrate such that the desired product is produced by enzymes in the cell, or the desired enzymes may be overproduced and purified from C. glutamicum cultures (or those of a related bacterium) and subsequently utilized in in vitro reactions in an industrial setting (either in solution or immobilized on a suitable immobile phase). In either situation, the enzyme can either be a natural C. glutamicum protein, or it may be mutagenized to have an altered activity; -64r, typical industrial uses for such enzymes include as catalysts in the chemical industry D for synthetic organic chemistry) as food additives, as feed components, for fruit processing, for leather preparation, in detergents, in analysis and medicine, and in the CN1 textile industry (see, e.g.,Yamada, H. (1993) "Microbial reactions for the production of useful organic compounds," Chimica 47: 5-10; Roberts, S.M. (1998) Preparative 0 biotransformations: the employment of enzymes and whole-cells in synthetic 00 0chemistry," J. Chem. Soc. Perkin Trans. 1: 157-169; Zaks, A. and Dodds, D.R. (1997) C "Application of biocatalysis and biotransformations to the synthesis of 0 pharmaceuticals," DDT2: 513-531; Roberts, S.M. and Williamson, N.M. (1997) "The 1 10 use of enzymes for the preparation of biologically active natural products and analogues in optically active form," Curr. Organ. Chemistry 1: 1-20; Faber, K. (1995) Biotransformations in Organic Chemistry, Springer: Berlin; Roberts, ed. (1992- 96) Preparative Biotransformations, Wiley: Chichester; Cheetham, P.S.J. (1995) "The applications of enzymes in industry" in Handbook of Enzyme Biotechnology, 3 r d ed., Wiseman, ed., Elis: Horwood, p. 419-552; and Ullmann's Encyclopedia of Industrial Chemistry (1987), vol. A9, Enzymes, p. 390-457). Thus, by increasing the activity or number of these enzymes, it may be possible to also increase the ability of the cell to convert supplied substrates to desired products, or to overproduce these enzymes for increased yields in large-scale culture. Further, by mutagenizing these proteins it may be possible to remove feedback inhibition or other repressive cellular regulatory controls such that greater numbers of these enzymes may be produced and activated by the cell, thereby leading to greater yields, production, or efficiency of production of these fine chemical proteins from large-scale cultures. Further, manipulation of these enzymes may alter the activity of one or more C. glutamicum metabolic pathways, such as those for the biosynthesis or secretion of one or more fine chemicals.

Mutagenesis of the proteolytic enzymes of the invention such that they are altered in activity or number may also directly or indirectly affect the yield, production, and/or efficiency of production of one or more fine chemicals from C. glutamicum. For example, by increasing the activity or number of these proteins, it may be possible to increase the ability of the bacterium to survive in large-scale culture, due to an increased ability of the cell to rapidly degrade proteins misfolded in response to the high temperatures, nonoptimal pH, and other stresses encountered during fermentor culture.

(11I Increased numbers of cells in these cultures may result in increased yields or efficiency of production of one or more desired fine chemicals, due to the relatively larger number of cells producing these compounds in the culture. Also, C. glutamicum cells possess rmultiple cell-surface proteases which serve to break down external nutrients into molecules which may be more readily incorporated by the cells as carbon/energy sources or nutrients of other kinds. An increase in activity or number of these enzymes 00 may improve this turnover and increase the levels of available nutrients, thereby Simproving cell growth or production. Thus, modifications of the proteases of the invention may indirectly impact C. glutamicum fine chemical production.

A more direct impact on fine chemical production in response to the modification of one or more of the proteases of the invention may occur when these proteases are involved in the production or degradation of a desired fine chemical. By decreasing the activity of a protease which degrades a fine chemical or a protein involved in the synthesis of a fine chemical it may be possible to increase the levels of that fine chemical (due to the decreased degradation or increased synthesis of the compound). Similarly, by increasing the activity of a protease which degrades a compound to result in a fine chemical or a protein involved in the degradation of a fine chemical, a similar result should be achieved: increased levels of the desired fine chemical from C. glutamicum cells containing these engineered proteins.

The aforementioned mutagenesis strategies for HA proteins to result in increased yields of a fine chemical from C. glutamicum are not meant to be limiting; variations on these strategies will be readily apparent to one of ordinary skill in the art. Using such strategies, and incorporating the mechanisms disclosed herein, the nucleic acid and protein molecules of the invention may be utilized to generate C. glutamicum or related strains of bacteria expressing mutated HA nucleic acid and protein molecules such that the yield, production, and/or efficiency of production of a desired compound is improved. This desired compound may be any product produced by C. glutamicum, which includes the final products of biosynthesis pathways and intermediates of naturally-occurring metabolic pathways, as well as molecules which do not naturally occur in the metabolism of C. glulamicum, but which are produced by a C. glutamicum strain of the invention.

-66- This invention is further illustrated by the following examples which should not Sbe construed as limiting. The contents of all references, patent applications, patents, published patent applications, Tables, and the sequence listing cited throughout this application are hereby incorporated by reference.

0 00 0- 0", 2006200802 24 Feb 2006 Table 1: Genes in the Application Nucleic Acid Amino Acid Identification Code Contig. NT Start NT Stop Function SEQ ID NO SEQ ID No 1 2 RXA02548 GR00727 3 293 SULFATE ADENYLATE TRANSFERASE SUBUNIT 2 (EC 2.7.7.4) 3 4 RXN00249 WV0057 36825 35869 ADENYLYLSULFATE KINASE (EC 2.7.1.25) 6 F RXA00249 GRO0037 8837 7884 ADENYLYLSULFATE KINASE (EC 2.7.1.25) 7 8 RXA01073 GROO300 1274 2104 NH(3)-DEPENDENT NAD(+) SYNTHETASE (EC 6.3.5.1) Urease Nucleic Acid Amino Acid Identification Code Cng. NT Start NT Stop Function SEQ ID NO SEQ IDNO 9 10 RXN02913 WV0020 8998 8513 UREASE BETA SUBUNIT (EC 3.5.1.5) 11 12 F RXA02264 GR00655 123 4 UREASE ALPHA SUBUNIT (EC 3.5.1.5) 13 14 RXN02274 W0020 8509 6800 UREASE ALPHA SUBUNIT (EC 3-5-1-5) 16 F RXA02274 GR00656 3 1604 UREASE ALPHA SUBUNIT (EC 3.5.1.5) 17 18 RXA02265 GR00655 452 153 UREASE GAMMA SUBUNIT (EC 3.5.1.5) 19 20 RXA02278 GROD656 3420 4268 UREASE OPERON URED PROTEIN 21 22 RXA02275 GR00656 1632 2102 UREASE ACCESSORY PROTEIN UREE 23 24 RXA02276 GR00656 2105 2782 UREASE ACCESSORY PROTEIN UREF 26 RXA02277 GR00656 2802 3416 UREASE ACCESSORY PROTEIN UREG 27 28 RXA02603 GR00742 7742 8737 4-I-YDROXYBENZOATE OCTAPRENYLTRANSFERASE (EC 2.5.1 29 30 RXA01385 GR00406 5320 3440 PHENOL 2 MONOOXYGENASE (EC 1.14.13.7) Proteolysis Nucleic Acid Amino Acid Identification Code Cni. NT Start NT Stop Function SEQ ID NO SEQ ID NO 31 32 RXN00675 W0005 33258 34049 METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) 33 34 F RXA00675 GRO01 78 2 484 METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) 36 RXA01609 GRO0449 2740 3612 METHIONINE AMINOPEPTIDASE (EC 3.4.11.18) 37 38 RXA01358 GR00393 5337 6857 ATP-DEPENDENT PROTEASE LA (EC 3.4.21.53) 39 40 RX(A01458 GR00420 3225 2176 ATP-DEPENDENT PROTEASE LA (EC 3.4.21.53) 41 42 RXA01654 GR00459 986 1981 (AL022121) putative alkaline serine protease (Mycobacterium tuberculosis) 43 44 RXN01868 W0127 9980 11905 ZINC METALLOPROTEASE (EC 3.4.24..) 46 F RXA01868 GR00534 1640 30 ZINC METALLOPROTEASE (EC 3.4.24..) 47 48 F RXA01869 GROO534 1954 1652 ZINC METALLOPROTEASE (EC 3,4.24.-) 49 50 RXN03028 W0008 41156 43930 ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPA 51 52 F RXA02470 GR00715 2216 3196 ATP-DEPENDENT CLP PROTEASE ATP-BINOING SUBUNIT CLPA 2006200802 24 Feb 2006 Table 1 (continued) Identification Code Cni. NT Start NT Stop Function Nuclec Acid Amino Acid SEQ ID NO SEQ IDNO 53 54 56 57 58 59 60 61 62 63 64 66 67 68 69 70 71 72 73 74 76 77 78 79 80 81 82 83 84 86 87 88 89 90 91 92 93 94 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 ill 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 F RXA02471 RXA02630 RXA02834 RXACO1 12 RXA00566 RXA00567 RXN03094 F RXA01668 RXN01 120 F RXA01 120 RXA00744 RXA00844 RXA01 151 RXA02317 RXA02644 RXN02820 F RXA02820 F RXA02000 RXN03178 F RXA02859 RXAC01 37 RXN00499 F RXA00499 RXN00877 F RXA00877 RXN01 014 F RXA01014 F RXA01018 RXAO1 147 RXAO1 161 RXN01 181 IFRXA01 181 RXN01 277 F RXA01277 RXAO1 914 RXA02048 RXN00621 F RXA00621 RXN00622 F RXA00622 RXN00982 F RXA00977 F RXA00982 RXA00l152 RXA02558 GR00715 3159 GR00748 2654 GR00823 3 GROO016 3687 GR00152 742 GR00152 1388 W0057 1794 GR00464 2205 W0182 5678 GROO31O0 2349 GR00202 10722 GR00228 3620 GR00324 862 GR00665 9664 GROO751 767 W0131 4799 GROO801 1 GR00589 3430 W0334 921 GR10005 846 GRO0022 738 W0086 8158 GR00125 3 W0099 2221 GR00242 3 W0209 13328 GR00289 3 GR00290 2289 GR00323 1353 GR00329 1253 W0065 1 GR00337 1 W0009 32155 GR00368 1738 GR00548 125 GR00624 207 W0135 5853 GR00163 4075 WV0135 5150 GR00163 4778 W0149 7596 GR00275 1647 GR00276 5194 GR00023 7175 GR00731 4939 4991 1332 497 2497 137 798 43 3920 4401 1072 9781 4453 5 9053 117 6109 507 3933 121 121 1826 9438 959 3885 1067 10728 1580 3152 94 117 957 957 34158 50 550 1580 5071 4857 3735 6193 6091 2660 4949 5880 3965 ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPA (AL021999) putative serine protease [Mycobacterium tuberculosis) ATPases with chaperone activity, ATP-dependent protease subunit PROBABLE PERIPLASMIC SERINE PROTEASE DO-LIKE PRECURSOR ATP-DEPENDENT CLIP PROTEASE PROTEOLYTIC SUBUNIT (EC 3.4.21.92) ATP-DEPENDENT CLP PROTEASE PROTEOLYTIC SUBUNIT (EC 3.4.21.92) CLPB PROTEIN CLPB PROTEIN ATP-DEPENDENT CLP PROTEASE ATP-BINDING SUBUNIT CLPX ATP-DEPENDENT CLIP PROTEASE ATP-BINDING SUBUNIT CLPX Periplasmic serine proteases Hypothetical Secretory Serine Protease (EC 3.4.21.-) ATP-dependent Zn proteases PEPTIDASE E (EC XAA-PRO DIPEPTIDASE (EC 3.4.13.9) GAMMA-GLUTAMYLTRANSPEPTIDASE (EC 2.3.2.2) GAMMA-GLUTAMYLTRANSPEPTIDASE (EC 2.3.2.2) GAMMA-GLUTAMYLTRANSPEPTIDASE (EC 2.3.2.2) PENICILLIN-BINDING PROTEIN 5* PRECURSOR (D-ALANYL-D-ALANINE CARBOXYPEPTIDASE) (EC 3.4.1 6.4) PENICILLIN-BINDING PROTEIN 5* PRECURSOR (D-ALANYL-D-ALANINE CARBOXYPEPTIDASE) (EC 3.4.16.4) XAA-PRO AMINOPEPTIDASE (EC 3.4.11.9) PROLINE IMINOPEPTIDASE (EC 3.4.11.5) PROLINE IMINOPEPTIDASE PEPTIDYL-DIPEPTIDASE DCP (EC 3.4.15.5) PEPTIDYL-DIPEPTIDASE DCP (EC 3.4.15.5) AMINOPEPTIDASE N (EC 3.4.11.2) AMINOPEPTIDASE N (EC 3.4.11.2) AMINOPEPTIDASE N (EC 3.4.11.2) VACUOLAR AMINOPEPTIDASE I PRECURSOR (EC 3.4.11.1) XAA-PRO AMINOPEPTIDASE (EC 3.4.11.9) AMINOP EPTIDASE All (EC 3.4.11.1)

AMINOPEPTIDASE

PROLY ENDOPEPTIDASE (EC 3.4.21.26) PROLM ENDOPEPTIDASE (EC 3.4.21.26)

AMINOPEPTIDASE

AMINOPEPTIDASE N (EC 3.4.11.2) PROTEASE 11 (EC 3.4.21.83) PTRB periplasmic protease PROTEASE 11 (EC 3.4.21.83) PTRB periplasmic protease (L42758) proteinase [Streptomyces lividans] (L42758) proteinase [Streptomyces lividans] (L42758) proteinase [Streptomyces lividans] HFLC; PROTEIN (EC I-FLC PROTEIN (EC 2006200802 24 Feb 2006 Table I (continued) Nucleic Acid Amino Acid Identification Code Contig NT Start NT Stop Function SEQ ID NO SEQ ID NO 143 144 RXA00500 GRO0125 969 1643 0-SIALOGLYCOPROTEIN ENDOPEPTIDASE (EC 3.4.24.57) 145 146 RXA00501 GR00125 1643 2149 0-SIALOGILYCOPROTEIN ENDOPEPTIDASE (EC 3.4.24.57) 147 148 RXA00502 GR00125 2156 3187 0-SIALOGILYCOPROTEIN ENDOPEPTIDASE (EC 3.4.24.57) Enzymes in general Nucleic Acid Amino Acid SEQ ID NO SEQ ID NO 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 1187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 Identification Code Contig. NT Start NT Step Function RXN02589 F RXA02589 RXA00226 RXN018685 F RXA01885 RXA02592 RXN1 795 F RXA01 795 RXA01214 RXA01 250 RXA02477 RXN00833 F RXA00833 RXA01 224 RXA01 182 RXA02531 RXNO0689 F RXA00689 RXN03128 F RXA02192 RXA02351 RXN00905 F RXA00905 RXA00906 RXA00907 RXA021O01 RXN02565 F RXA02565 F RXA02567 RXN03077 F RXA02855 RXA00026 RXA01971 RXA01802 RXN00866 F RXAC0866 RXA0241 0 WV0098 16346 GR00741 13804 GR00032 26836 W0184 2004 GR00539 1589 GR00741 18477 W0093 722 GR00507 706 GR00351 1640 GR00364 592 GR00715 10581 GR00225 374 GR00225 374 GR00354 4186 GR00337 1363 GR00726 1226 W0005 22416 GR00180 1401 W0120 3 GR00643 2 GR00679 132 VV0238 8075 GR00247 2 GR00247 630 GR00247 1143 GR00631 3104 VV0154 14299 GR00733 1 GR00734 3 W0043 1729 GR10002 1693 GROO003 3657 GR00569 963 GR00509 3461 W0258 3557 GR00236 3555 GR00703 792 17110 13040 26012 2804 2389 17707 1318 1140 3130 5 11201 6 6 5208 9711 1936 20926 775 857 523 1070 8875 694 1133 1265 1842 13034 342 740 2913 2877 5042 133 4291 4522 4499 127 Hypothetical Methyltransfe rase (EC Predicted S-adenosytmethionine-dependent methyltransferase SAM-dependent methyltransferases Hypothetical Methyltransferase (EC 2. 1. SAM-dependent methyltransferases SAM-dependent methyltransferases MODIFIKATION METH-YLASE (EC 2.1.1.73) MODIFICATION METHYLASE (EC 2.1.1.73) LACCASE 1 PRECURSOR (EC 1.10.3.2) LACCASE 1 PRECURSOR (EC 1. 10.3.2) CARBONIC ANHYDRASE (C 4.2.1. 1) THIOL PEROXIDASE (EC 1.11.1.-) THIOL PEROXIDASE (EC 1.11. 2-NITROPROPANE DIOXYGENASE (EC 1.13.11.32) Hypothetical Oxidoreductase Hypothetical Oxidareductase BETAINE-ALDEI-YDE DEI-YOROGENASE PRECURSOR (EC 1.2.1.8) BETAINE-ALDEHYDE DEHYDROGENASE PRECURSOR (EC 1.2-1.8) MORPHINE 6-DEHYDROGENASE (EC 1.1.1.218) MORPHINE 6..DEHYDROGENASE (EC 1.1.1.218) NITRILOTRIACETATE MONOOXYGENASE COMPONENT A (EC 1.14.13.-) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROI.ASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYOROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO, ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14), hippurate hydrolase Hypothetical Amnidohydrolase (EC Hypothetical Metal-Dependent Hydrolase Predicted hydrolases (HAD superfamily) Predicted Zn-dependent hydrolases Predicted Zn-dependent hydrolases Predicted Zn-dependent hydrolases 2006200802 24 Feb 2006 Table I (continued) NT Start NT Stop Function Nucleic Acid SEQ 10 NO 223 225 227 229 231 233 235 237 239 241 243 245 247 249 251 253 255 257 259 261 263 265 267 Amino Acid SEQ ID NO 224 226 228 230 232 234 236 238 240 242 244 246 248 250 252 254 256 258 260 262 264 266 268 Identification Code RXAG0961 RXA001 11 RXA01 932 RXA02574 RXNOD983 F RXA00983 RXA00984 RXN02513 F RXA02513 RXA00903 RXA01 224 RXA01 571 RXN02478 RXN00343 RXN01 555 RXNO1 166 RXN02001 RXN03145 RXN01466 RXN01 145 RXN03088 RXN02952 RXN0051 3 Co2!!2.

GR00267 GR0001 6 GR00555 GR00739 WV0231 GR00278 GR00278 WV01 93 GR00722 GR00246 GR00354 GR00438 wolig1 WV0125 01 35 W01 17 W0326 WV0142 W0019 W0077 W0052 W0320 W0092 W0136 W0321 2 930 6479 833 1796 1200 1716 737 93 637 4186 1360 7564 1118 29820 18142 630 7561 7050 7538 3431 1032 1573 1740 3736 433 1922 5583 1840 321 4 1300 6 824 5 5208 1959 6350 6 28861 16838 1787 7115 6091 6525 3817 1547 653 907 5637 SALICYLATE HYDROXYLAS E (EC 1. 14.13. 1) SOLUBLE EPOXIDE HYDROLASE (SEHI) (EC 3.3.2.3) ACETYL-HYDROLASE (EC PUTATIVE SECRETED HYOROLASE SIALIDASE PRECURSOR (EC3.2.1.18) SIALIDASE PRECURSOR (EC 3.2.1.18) SIALIDASE PRECURSOR (EC 3.2.1.18) SIALIDASE PRECURSOR (EC; 3.2.1.18) SIALIDASE PRECURSOR (EC 3.2.1.18) Putative epimerase 2-NITROPROPANE DIOXYGENASE (EC 1.13.11.32) ALCOHOL DEHYDROGENASE (EC 1111 SIALIDASE PRECURSOR (EC 3.2.1.18) 3-OXOSTEROID 1-DEHYDROGENASE (EC 1.3.99.4) 3-OXOSTEROID 1 -DEHYDROCENASE (EC 1.3.99.4) EXTRACELLULAR. LIPASE PRECURSOR (EC 3.1.1.3) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) 4-OXALOCROTONATE TAUTOMERASE (EC ARYLESTERASE (EC 3.1.1.2) KETOL-ACID REDUCTOISOMERASE (EC 1.1.1.86) Hypothetical Methyltransferase (EC PUTATIVE REDUCTASE CARBOXYVINYL-CARBOXYPHOSPHONATE PHOSPHORYLMUTASE (EC 2.7.8.23) PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE (EC 2.1.1.77) 0-AMINO ACID DEHYDROGENASE LARGE SUBUNIT (EC 1.4.99. 1) 271 272 N-metabolism RXN01152 RXN00787 Nucleic Acid SEQ ID NO 273 275 277 279 281 283 285 287 289 291 293 295 297 Amino Acid SEQ ID NO 274 276 278 280 282 284 286 288 290 292 294 296 298 Identification Code RXN01302 F RXA01 302 RXN01 308 F RXA01 307 F RXA01 308 RXN01309 F RXA01 309 RXA0201 7 RXA0201 8 RXA0201 6 RXA00471 RXAC01 33 RXA00650 Contig W0148 CR00376 W0148 GR00377 GR00378 W01 58 GR00379 GR00610 GR00610 CR00610 GR00119 GR00021 GR00169 NT Start 2837 370 2406 686 1211 719 1731 2788 1036 2997 201 4017 NT Stop 2385 5 4 6 6 801 51 1048 1739 260 3686 1013 3382 Function NITRATE REDUCTASE ALPHA CHAIN (EC 1.7.99.4) NITRATE REDUCTASE ALPHA CHAIN (EC 1.7.99.4) NITRATE REDUCTASE ALPHA CHAIN (EC 1.7.99.4) NITRATE REDUCTASE ALPHA CHAIN (EC 1.7.99.4) NITRATE REDUCTASE ALPHA CHAIN (EC 1.7.99.4) NITRATE REDUCTASE ALPHA CHAIN (EC 1.7.99.4) NITRATE REDUCTASE ALPHA CHAIN (EC 1.7.99.4) NITRATE REDUCTASE ALPHA CHAIN (EC 1.7.99.4) NITRATE REDUCTASE BETA CHAIN (EC 1.7.99.4) NITRATE REDUCTASE GAMMA CHAIN (EC 1.7.99.4) NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARL NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARP NITRATE/NITRITE RESPONSE REGULATOR PROTEIN NARP 2006200802 24 Feb 2006 Table 1 (continued) NT Start NT Stop Function Nucleic Acid SEQ ID NO 299 301 303 305 307 309 311 313 Amino Acid SEQ ID NO 300 302 304 306 308 310 312 314 Identification Code Contig.

RXA01 189 RXA01607 RXN00470 F RXA00470 RXA00756 RXA001 39 RXA01303 RXA01412 RXA00773 RXA02746 RXA02745 RXN00820 F RXA00820 RXA0 1059 RXN01386 RXN00073 RXN03131 RXS001 53 GR00339 GR00449 WO0086 GRO0119 GR00203 GROO022 GR00376 GROO41 2 GR00205 GR00764 GR00763 VVD054 GR00221 GR00296 WV0008 WV0154 WV0127 WV0167 2545 123 27401 1762 2932 2514 1724 620 3208 15350 19455 1007 8782 39246 2369 276 4195 1937 752 28669 2951 1937 3224 390 417 4350 267 14.472 19817 1369 9390 38317 687 4 4620 NITRATEINITRITE RESPONSE REGULATOR PROTEIN NARP NITRATEJNITIRITE RESPONSE REGULATOR PROTEIN NARP NITRATEINITRITE SENSOR PROTEIN NARX (EC NITRATEINITRITE SENSOR PROTEIN NARX (EC N UTILIZATION SUBSTANCE PROTEIN A N UTILIZATION SUBSTANCE PROTEIN B NITRITE EXTRUSION PROTEIN NITROGEN FIXATION PROTEIN FIX[ (PROBABLE E1I-E2 TYPE CATION ATPASE) (EC 3.6. NITROGEN REGULATION PROTEIN NIFR3 NITROGEN REGULATORY PROTEIN P-il NODULATION ATP-BINDING PROTEIN I NODULATION PROTEIN N NODULATION PROTEIN N OXYGEN-INSENSITIVE NAD(P)H NITROREDUCTASE (EC NITRILASE REGULATOR FERREDOXIN-NITRITE REDUCTASE (EC 1.7.7.1) RHIZOPINE CATABOLISM PROTEIN MOCC NODULATION PROTEIN Urease Nucleic Acid Amino Acid SEQ ID NO SECIID NO Identification Code Contig NT Start NT Stop Function Phosphate and Phosphonate metabolism Nucleic Acid SEQ ID NO 335 337 339 341 343 Amino Acid Identification Code Contig.

SEQ ID NO 336 RXN01716 W031 9 338 RXN02972 W0319 340 RXN00663 W0142 342 RXN00778 W01 03 344 RXN00250 W0189 NT Start NT Stop Function 3259 2763 10120 18126 286 2774 2353 11493 19250 1032 EXOPOLYPHOSPHATASE (EC 3.6.1.11) EXOPOLYPHOSPH-ATASE (EC 3.6.1.11) PHOH PROTEIN HOMOLOG PHOSPHATE-BINDING PERIPLASMIC PROTEIN PRECURSOR DEDA PROTEIN ALKALINE PHOSPHATASE LIKE PROTEIN Sulfate metabolism Nucleic Acid SEQ ID NO 345 347 349 Amino Acid Identification Code Contig.

SEQ ID NO 346 RXA00072 GROO01 2 348 RXA00793 GROO211 350 RXAO1 192 GR00342 NT Start NT Stop Function 446 1469 161 6 PHOSPI-IADENOSINE PHOSPHOSULFATE REDUCTASE (EC 1.8.99.4) 2644 SULFATE STARVATION-INDUCED PROTEIN 6 733 SULFATE STARVATION-INDUCED PROTEIN 6 2006200802 24 Feb 2006 Nucleic Acid SEQ ID NO 351 353 355 357 Amino Acid Identification Code Contig.

SEQ ID NO 352 RXA00715 GRO0188 354 RXA01664 GR00463 356 RXN02334 W0141 358 F RXA02334 GR00672 Table I (continued) NT Start NT Stop Function 2120 2914 TH-IOSULFATE SULFURTRANSFERASE (EC 2.8.1.1) 1306 485 THIOSULFATE SULFURTRANSFERASE (EC 2.8.1.1) 7939 7217 THIOSULFATE SULFURTRANSFERASE (EC 2.8.1.1) 2 355 THIOSULFATE SULFURTRANSFERASE (EC 2.8.1.1) Fe-Metabolism Nucleic Acid SEQ ID NO 359 361 Amino Acid Identification Code Contig SEQ ID NO 360 RXN01499 W0008 362 RXN01997 W0084 NT Start NT Stop Function 7034 3213 ENTEROBACTIN SYNTHETASE COMPONENT F 33308 33793 FERRITIN Mg Metabolism Nucleic Acid SEQ ID NO 363 365 367 369 371 Amino Acid Identification Code Contigj SEQ ID NO 364 RXAO 1848 GR00524 366 RXN01849 W0139 368 F RXA01849 GR00524 370 F RXA01691 GR00474 372 RXN00665 W0252 NT Start NT Stop 1532 16415 2004 570 135 789 17515 1555 4 635 Function MAGNESIUM-CHELATASE SUBUNIT CHLI MAGNESIUM-CHELATASE SUBUNIT CHLI MAGNESIUM-CHELATASE SUBUNIT CHLI MAGNESIUM-CHELATASE SUBUNIT CHLI MG2+ICITRATE COMPLEX SECONDARY TRANSPORTER Modification and degradation of aromatic compounds Nucleic Acid SEQ ID NO 373 375 377 379 381 383 385 387 389 391 393 395 397 399 401 403 405 407 Amino Acid Identification Code Contig SEQ ID NO 374 RXN03026 W0007 376 RXN02908 W0025 378 RXN03000 W0235 380 RXND3036 W0014 382 RXN02974 W0229 384 RXN00393 W0025 386 RXN00948 W0107 388 RXN01923 W0020 390 RXN00398 W0025 392 RXN02813 W0128 394 RXNOO1 36 W0134 396 RXN02508 W0007 398 RXN02839 W0362 400 RXN00639 W0128 402 RXN02530 W0057 404 RXN00434 W01 12 406 RXN01619 W0050 408 RXN01842 W0234 NT Start NT Stop 28635 8507 570 671 12631 7241 4266 3384 14633 13120 13373 26733 3 7858 5469 12078 24649 1615 28901 8247 4 6 12437 6348 5384 4133 13884 14118 14467 28586 449 8712 6125 11212 23675 2532 Function 3-DEHYDROQUINATE DEHYDRATASE (EC 4.2.1.10) O-SUCCINYLBENZOIC ACID-COA LIGASE (EC 6.2.1.26) SALICYLATE HYDROXYLASE (EC 1.14.13.1) PROTOCATECHUATE 3,4-DIOXYGENASE BETA CHAIN (EC 1.13.11.3) 4-NITR0PHJENYLPHOSPHATASE (EC 3.1.3.41) 1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE (EC 12-oxophytodienoate reductase (EC 1.3.1.42) 2-HYDROXY-6-OXO-6-PHENYLHEXA-2,4-DIENOATE HYDROLASE (EC 2-PYRONE-4,6-DICARBOXYLATE LACTONASE (EC 3.1.1.57) 3-CARBOXY-CIS,CIS-MUCONATE CYCLOISOMERASE H-OMOLOG (EC 5.5.1.2) 3-DEHYDROQUINATE SYNTHASE (EC 4.6.1.3) 3-DEHYDROSHIKIMATE DEHYDRATASE (EC 4-H-YDROXYBENZOATE OCTAPRENYLTRANSFERASE (EC CATECIIOL 1,2-DIOXYGENASE (EC 1. 13.11' 1) DIMETHYLANILINE MONOOXYGENASE (N-OXIDE FORMING) 1 (EC 1.14.13.8) QUINONEOXIDOREDUCTASE (EC 1.6.5.5) QUINONE OXIDOREDUCTASE (EC 1.6.5.5) QUINONE OXIDOREOUCTASE (EC 1.6.5.5) 2006200802 24 Feb 2006 Table I (continued) NT Start NT Stop Function Nucleic Acid SEQ ID NO 409 411 413 415 417 419 421 423 425 Amino Acid Identification Code Con~t SEQ ID NO 410 RXN00641 W0128 412 RXN01993 WV0182 414 RXN00658 WV0083 416 RXN00178 W0O174 418 RXN01461 W0128 420 RXN01653 WV0321 422 RXN02053 WV0009 424 RXN00177 W0174 426 RXCO0963 W0249 7440 16 15705 14670 12414 12867 39448 13589 1816 5950 1143 16397 15554 13025 11407 40026 14656 2652 TOLUATE 1,2-DIOXYGENASE ALPHA SUBUNIT (EC 1.14.12.-) VANILLATE DEMETHYLASE (EC PHENOL 2-MONOOXYGENASE (EC 1. 14.13.7) hydroxyquinol 1,2-dioxygenase (EC 1.13.11.37) PROTOCATECHUATE 3,4-DIOXYGENASE ALPHA CHAIN (EC 1.13.113) DIBENZOTHIOPHENE DESULFURIZATION ENZYME A DRGA PROTEIN MALEYLACETATE REDUCTASE (EC 1.3.1.32) PROTEIN involved in degradation of aromatic compounds Modification and degradation of aliphatic compounds Nucleic Acid SEQ ID NO 427 429 431 433 435 437 439 Amino Acid Identification Code Contig.

SEQ ID NO 428 RXN00299 W0176 430 F RXA00299 GR00048 432 RXA00332 GR00057 434 RXA01838 GR00519 436 RXA02643 GROO750 438 RXA01933 GR00555 440 RXA02351 GR00679 NT Start NT Stop Function- 43379 7376 16086 2 1603 6590 132 42402 6633 15385 820 560 7192 1070 ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) ALKANAL MONOOXYGENASE ALPHA CHAIN (EC 1.14.14.3) 2-HALOALKANOIC ACID DEHALOGENASE I (EC 3.8.1.2) NITRILOTRIACETATE MONOOXYGENASE COMPONENT A (EC 1.14.13.-) 2006200802 24 Feb 2006 2 Excluded Genes Gen~ank~m Gene Name Gene Function Reference Accession No.

A09073 ppg Phosphoenol pyruvate carboxylase Bachmann, B. et al. "DNA fragment coding for phosphoenolpyruvat corboxylase, recombinant DNA carrying said fragment, strains carrying the recombinant DNA and method for producing L-aminino acids using said strains," Patent: EP 0358940-A 3 03/21/90 A45579, Threonine dehydratase Moeckel, B. et al. "Production of L-isoleucine by means of recombinant A45581, micro-organisms with deregulated threonine dehydratase," Patent: WO A45583, 9519442-A 5 07/20195 A45585 AB003 132 murC; ftsQ; ftsZ Kobayashi, M. et al. "Cloning, sequencing, and characterization of the ftsZ gene from coryneform bacteria," Biochem. Biophys. Res. Commun., (1997) ABO15023 murC; ftsQ Wachi, M. et al. "A murC gene from Coryneform bacteria," AppL MicrobioL 5](2):223-228 (1999) ABO18530 dtsR Kimura, E. et al. "Molecular cloning of a novel gene, dtsR, which rescues the detergent sensitivity of a mutant derived from Brevibacterium ____________lactofermentum," Biosci. BiolechnoL. Biochem., 60(10):1565-1570 (1996) ABO18531 dtsRI; dtsR2 AB020624 murl D-glutamate rac emase AB023377 tkt transketolase AB024708 g~tB; gltD Olutamine 2-oxoglutarate aminotransferase and small subunits AB025424 acn aconitase AB027714 rep Replication protein AB027715 rep; aad Replication protein; aminoglycoside ______________adenyltransferase AF005242 argC ~dehydrogenase AF005635 gInA Glutamine synthetase AF030405 hisF cyclase AF030520 argG Argininosuccinate synthetase AF03 1518 argF Ornithine carbamolytransferase_________ AF036932 aroD 3-dehydroquinate dehydratase AF038548 pyc Pyruvate carboxylase 2006200802 24 Feb 2006 Table 2 (continued) AF038651 dciAE; apt; rel Dipeptide-binding protein; adenine Wehmeier, L. et al. "The role of the Corynebacterium glutamicum rel gene in phosphoribosyltransferase; GTP (p)ppGpp metabolism," Microbiology, 144:1853-1 862 (1998) ________________pyrophosphokinase AF041436 argR Arginine repressor AF045998 imnpA Inositol monophosphate phosphatase AF048764 argH Argininosuccinate lyase AF049897 argC; argi; argB; N-acetylglutamnylphosphate reductase; argD; argF; argR; ornithine acetyltransferase; NargG; argi- acetylgiutamnate kinase; acetylornithine transminase; omithine carbamoyltransferase; arginine repressor; argininosuccinate synthase; argininosuccinate lyase AF050 109 inhA Enoyl-acyl carrier protein reductase AF050 166 hisG ATP phosphoribosyltransferase 1846 hisA Phosphoribosylformimino-5-amino- 1phosphoribosyl-4-imidazolecarboxamide isomerase AF052652 metA Homnoserine 0-acetyltransferase Park, S. et al. "Isolation and analysis of metA, a methionine biosynthetic gene encoding homoserine acetyltransferase in Corynebacterium glutamicum," Mol.

Cells., 8(3):286-294 (1998) AF053071 arol3 Dehydroquinate synthetase AF060558 hisH Glutamine amidotransferase AF086704 hisE Phosphoribosyl-ATP- _________________pyrophosphohydrolase AFR 14233 aroA 5-enolpyruvyishikimate 3-phosphate synthase AF 16184 panD L-aspartate-alpha-decarboxy lase precursor Dusch, N. et al. "Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli," Appi. Environ. Microbiol., 65(4)1530- 1539 (1999) AF124518 aroD; aroE 3-dehydroquinase; shikimate dehydrogenase AF124600 aroC; aroK; aroB; Chorismate synthase; shikimate kinase; 3pepQ dehydroquinate synthase; putative cytoplasmic peptidase AF145897 AF145898 inhA 2006200802 24 Feb 2006 2 (continued) AJQO1436 ectP Transport of ectoine, glycine betaine, Peter, et al. "Corynebacterium glutamicum is equipped with four secondary proline carriers for compatible solutes: Identification, sequencing, and characterization of the proline/ectoine uptake system, ProP, and the ectoine/proline/glycine carrier, EctP," J. Bacteriol., I 80(22):6005-60 12 (1998) AJO04934 dapD Tetrahydrodipicolinate succinylase Wehrmnann, A. et al, "Different modes of diamninopimelate synthesis and their (incomplete') role in cell wall integrity: A study with Corynebacteriumn glutamicum," J 180(1 2):3 159-3165 (1998) AJ007732 ppc; secG; amt; ocd; Phosphoenolpyruvate-carboxylase; high soxA affinity ammonium uptake protein; putative orn ith ine-cyc lodecarboxy lase; sarcosine oxidase AJO10319 ftsY, glnB, gInD; srp; Involved in cell division; PH1 protein; Jakoby, M. et al. "Nitrogen regulation in Corynebacterium glutamicum; amtP uridylyltransferase (uridylyl-removing Isolation of genes involved in biochemical characterization of corresponding enzmnye); signal recognition particle; low proteins," FEMS Microbial, 173(2):303-310 (1999) affinity ammonium uptake protein AJ132968 cat Chioramphenicol aceteyl transferase AJ224946 mqo L-malate: quinone oxidoreductase Molenaar, D. et al. "Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium Eur. J Biochem., 254(2):395-403 (1998) AJ238250 ndh NADH dehydrogenase AJ238703 porA Porin Lichtinger, T. et al. "Biochemical and biophysical characterization of the cell wall porin of Corynebacteriumn glutamicum: The channel is formed by a low mass polypeptide," Biochemistry, 37(43): 15024-15032 (1998) D1 7429 Transposable element 1S3 1831 Vertes et al."Isolation and characterization of IS3 183 1, a transposable element Corynebacterium glutamnicum," Mo!. Microbiol., 11 (4):739-746 (1994) D84 102 odhA 2-oxoglutarate dehydrogenase Usuda, Y. et al. "Molecular cloning of the Corynebacterium glutamicumn (Brevibacterium lactofermentumn AJ 12036) odhA gene encoding a novel type 2-oxoglutarate dehydrogenase," Microbiology, 142:3347-3354 (1996) EQ01358 hdh; hk Homoserine dehydrogenase; homoserine Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent: JP 1987232392-A 1 10/12/87 E01359 Upstream of the start codon of homoserine Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent: JP gene 1987232392-A 2 10/12/87 E0 1375 Tryptophan operon EQ01376 trpL; trpE Leader peptide; anthranilate synthase Matsui, K. et al. "Tryptophan operon, peptide and protein coded thereby, utilization of tryptophan operon gene expression and production of Patent: JP 1987244382-A I 10/24/87 2006200802 24 Feb 2006 Table 2 (continued) E01377 Promoter and operator regions of Matsui, K. et al. "Tryptophan operon, peptide and protein coded thereby, tryptophan operon utilization oftryptophan operon gene expression and production of tryptophan," Patent: JP 1987244382-A 1 10/24/87 E03937 Biotin-synthase Hatakeyama, K. et al. "DNA fragment containing gene capable of coding biotin synthetase and its utilization," Patent: JP 1992278088-A 1 10/02/92 E04040 Diamino pelargonic acid aminotransferase Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A 1 11/18/92 E04041 Desthiobiotinsynthetase Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A 1 11/18/92 E04307 Flavum aspartase Kurusu, Y. et al. "Gene DNA coding aspartase and utilization thereof," Patent: JP 1993030977-A 1 02/09/93 E04376 Isocitric acid lyase Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 1993056782-A 3 03/09/93 E04377 Isocitric acid lyase N-terminal fragment Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 1993056782-A 3 03/09/93 E04484 Prephenate dehydratase Sotouchi, N. et al. "Production of L-phenylalanine by fermentation," Patent: JP 1993076352-A 2 03/30/93 E05108 Aspartokinase Fugono, N. et al. "Gene DNA coding Aspartokinase and its use," Patent: JP 1993184366-A 1 07/27/93 E05112 Dihydro-dipichorinate synthetase Hatakeyama, K. et al. "Gene DNA coding dihydrodipicolinic acid synthetase and its use," Patent: JP 199318437 I-A 1 07/27/93 E05776 Diaminopimelic acid dehydrogenase Kobayashi, M. et al. "Gene DNA coding Diaminopimelic acid dehydrogenase and its use," Patent: JP 1993284970-A 1 11/02/93 E05779 Threonine synthase Kohama, K. et al. "Gene DNA coding threonine synthase and its use," Patent: JP 1993284972-A 1 11/02/93 E06110 Prephenate dehydratase Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," Patent: JP 1993344881-A 1 12/27/93 E06111 Mutated Prephenate dehydratase Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," Patent: JP 1993344881-A I 12/27/93 E06146 Acetohydroxy acid synthetase Inui, M. et al. "Gene capable of coding Acetohydroxy acid synthetase and its use," Patent: JP 1993344893-A 1 12/27/93 E06825 Aspartokinase Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A 1 03/08/94 E06826 Mutated aspartokinase alpha subunit Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A 1 03/08/94 2006200802 24 Feb 2006 Table 2 (continued) E06827 Mutated aspartokinase alpha subunit Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A I E07701 secY H-onno, N. et al. "Gene DNA participating in integration of membraneous protein to membrane," Patent: JP 1994169780-A 1 06/21/94 E08 177 Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase released from feedback inhibition and its utilization," Patent: JP 1994261766-A 1 09/20/94 E08 178, Feedback inhibition-released Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase released from E08 179, feedback inhibition and its utilization," Patent: JP 1994261766-A 1 09/20/94 E08 180, E08 181, E08 182 E08232 Acetohydroxy-acid isomeroreductase lnui, M. et al. "Gene DNA coding acetohydroxy acid isomeroreductase," JP 1994277067-A 1 10/04/94 E08234 secE Asai, Y. et al. "Gene DNA coding for translocation machinery of protein," JP 1994277073-A 1 10/04/94 E08643 FT aminotransferase and desthiobiotin Hatakeyama, K. et al. "DNA fragment having promoter function in synthetase promoter region coryneform bacterium," Patent: JP 1995031476-A 1 02/03/95 E08646 Biotin synthetase Hatakeyama, K. et al. "DNA fragment having promoter function in bacterium," Patent: JP 1995031476-A 1 02/03/95 E08649 Aspartase Kohama, K. et al "DNA fragment having promoter function in coryneformn Patent: JP 1995031478-A 1 02/03/95 E08900 Dihydrodipicolinate reductase Madori, M. et al. "DNA fragment containing gene coding Dihydrodipicolinate acid reductase and utilization thereof," Patent: JP 1995075578-A 1 03/20/95 E08901 Diaminopimelic acid decarboxylase Madori, M. et al. "DNA fragment containing gene coding Diaminopimelic acidand utilization thereof," Patent: JP 1995075579-A 1 03/20/95 E12594 Serine hydroxymethyhtransferase Hatakeyama, K. et al. "Production of L-trypophan," Patent: JP 1997028391 -A 02/04/97 E 12760, transposase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: E12759, JP 1997070291 -A 03/18/97 BE12758 E 12764 Arginyl-tRNA synthetase; diaminopimelic Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: decarboxylase J P 199707029 I -A 03/18/97 BE12767 Dihydrodipicolinic acid synthetase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: P 199707029 1-A 03/18/97 B 12770 aspartokinase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: P 1997070291 -A 03/18/97 BE12773 Dihydrodipicolinic acid reductase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: JP 1997070291 -A 03/18/97 2006200802 24 Feb 2006 Table 2 (coninued) E 13655 GlIucose-6 -phosphate dehydrogenase Hatakeyama, K. et al. "Glucose-6-phosphate dehydrogenase and DNA capable coding the same," Patent: JP 199722466 1-A 1 09/02/97 LO01508 1IlvA Threonine dehydratase Nloeckel, B. et al. "Functional and structural analysis of the threonine dehydratase of Corynebacterium glutamicum," J. Bacterial., 174:8065-8072 (1992) L07603 EC 4.2.1.15 3-deoxy-D-arabinoheptulosonate-7- Chen, C. et al. "The cloning and nucleotide sequence of Corynebacterium phosphate synthase glutamicum 3-deoxy- D-arabinoheptulIoson ate- 7-ph osphate synthase gene," Microbial. Len., 107:223-230 (1993) L09232 llvB; ilvN; ilvC Acetohydroxy acid synthase large subunit; Keilhauer, C. et al. "Isoleucine synthesis in Corynebacterium glutamicum: Acetohydroxy acid synthase small subunit;- molecular analysis of the ilvB-ilvN-ilvC operon," J. Bacterial., 175(17):5595acid isomeroreductase 5603 (1993) L 18874 PtsM Phosphoenolpyruvate sugar Fouet, A et al. "Bacillus subtilis sucrose-specific enzyme 11 of the phosphotransferase phosphotransferase system: expression in Escherichia coli and homology to enzymes If from enteric bacteria," PNAS USA, 84(24):8773-8777 (1987); Lee, J.K. et al. "Nucleotide sequence of the gene encoding the Corynebacteriumn glutamicum mannose enzyme If and analyses of the deduced protein FEMS Microbial Let., 1 19(1-2):137-145 (1994) L27 123 aceB Malate synthase Lee, H-S. et al. "Molecular characterization of aceB, a gene encoding malate synthase in Corynebacterium glutamicum," J. Mlicrobial. BiotechnaL, (1994) L27 126 Pyruvate kinase Jetten, M. S. et al. "Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum," Appl. Environ. Microbiol., 60(7):2501-2507 L28760 aceA Isocitrate lyase L35906 dtxr Diphtheria toxin repressor Oguiza, J.A. et al. "Molecular cloning, DNA sequence analysis, and characterization of the Corynebacteriumn diphtheriae dtxR from Brevibacterium ____________lactofermentum," J Bacterial., 177(2):465-467 (1995) M13774 Prephenate dehydratase Follettie, M.T. et al. "Molecular cloning and nucleotide sequence of the ___________Corynebacteriumn glutamicum pheA gene," J Bacterial, 167:695-702 (1986)_ M 16175 5S rRNA Park, Y-H. et al. "Phylogenetic analysis of the coryneform bacteria by 56 sequences," J Bacterial, 169:1801 -1806 (1987) M 16663 trpE Anthranilate synthase, 5' end Sano, K. et al. "Structure and function of the trp operon control regions of Brevibacterium lactofermentum, a glutamnic-acid-producing bacterium," Gene, (1987) M 16664 trpA Tryptophan synthase, 3'end Sano, K. et al. "Structure and function of the trp operon control regions of Brevibacterium lactofermentum, a glutam ic-acid-producing bacterium," Gene, (1987) 2006200802 24 Feb 2006 2 (continued) M25819 Phosphoenolpyruvate carboxylase O'Regan, M. et al. "Cloning and nucleotide sequence of the Phosphoenolpyruvate carboxylase-coding gene of Corynebacterium ATCC 13032," Gene, 77(2):237-25 1 (1989) 106 23S rRNA gene insertion sequence Roller, C. et al. "Gram-positive bacteria with a high DNA G+C content are characterized by a common insertion within their 23S rRNA genes," J. Gen.

Mirobiol., 138:1167-1 175 (1992) 107, 23S rRNA gene insertion sequence Roller, C. et "Gram-positive bacteria with a high DNA G+C content are 108 characterized by a common insertion within their 23S rRNA genes," J Gen.

138:1167-1175 (1992) M89931I aecD; brnQ; yhbw Beta C-S lyase; branched-chain amino acid Rossol, 1. et al. "The Corynebacterium glutamicum aecD gene encodes a C-S uptake carrier; hypothetical protein yhbw lyase with alpha, beta-elimination activity that degrades aminoethylcysteine," J. Bacteriol., 174(9):2968-2977 (1992); Tauch, A. et al. "Isoleucine uptake in Corynebacterium glutamnicum ATCC 13032 is directed by the brnQ gene Arch. MicrobiaL, 1 69(4):303-3 12 (1998) S59299 trp Leader gene (promoter) Herry, D.M. et al. "Cloning of the tip gene cluster from a tryptophanhyperproducing strain of Corynebacterium glutamicum: identification of a mutation in the tip leader sequence," Appl. Environ. Microbial., 59(3):791-799 1993) UI 11545 trpD Anthranilate phosphoribosyltransferase O'Gara, J.P. and Dunican, L.K. (1994) Complete nucleotide sequence of the Corynebacterium glutamicum ATCC 21850 tpD gene." Thesis, Microbiology Department, University College Galway, Ireland.

U 13922 eglIM; cglIR; clglIR Putative type 11 5-cytosoine Schafer, A. et al. "Cloning and characterization of a DNA region encoding a methyltransferase; putative type 11 stress-sensitive restriction system from Corynebacterium glutamicum ATCC restriction endonuclease; putative type I or 13032 and analysis of its role in intergeneric conjugation with Escherichia type Ill restriction endonuclease coli," J Bacleriol, 176(23):7309-7319 (1994); Schafer, A. et "The Coiynebacterium glutamicum cgllM gene encoding a 5-cytosine in an McrBCdeficient Escherichia coli strain," Gene, 203(2);95-101 (1997) U 14965 recA U31224 pX Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline, biosynthetic pathway: A natural bypass of the proA step," J Bacterial., 178(15):4412-4419 (1996) U3 1225 proC L-proline: NADP+ 5-oxidoreductase Ankri, S. et al. "Mutations in the Corynebacterium glutamnicumproline biosynthetic pathway: A natural bypass of the proA step," J. Bacterial., 178(15):4412-4419 (1996) U3 1230 obg; proB; unkdh ?;gamma glutamyl kinase;similar to D- Ankri, S. et al. "Mutations in the Corynebacterium glutamnicumproline isomer specific 2-hydroxyacid biosynthetic pathway: A natural bypass of the proA step," J Bacterial, _____________dehydrogenases 178(15):4412-4419 (0996) 2006200802 24 Feb 2006 Table 2 (continued) U31281 bioB Biotin syntha-se Serebriiskii, "Two new members of the bio B superfamily: Cloning, sequencing and expression of bio B genes of Methylobacillus flagellatum and Corynebacterium glutamicum," Gene, 175:15-22 (1996) U35023 thtR; accBC Thiosulfate sulfurtransferase; acyl CoA lager, W. et al. "A Corynebacterium glutamicum gene encoding a two-domain carboxylase protein similar to biotin carboxylases and biotin-carboxyl-carrier proteins," Microbiol., 166(2);76-82 (1996) U43535 cmr Multidrug resistance protein Jager, W. et al. "A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli," J. Bacteriol., 179(7):2449-2451I (1997) U43536 clpB Heat shock ATP-binding protein U53587 aphA-3 3'5' -aminoglycoside phosphotransferase U89648 Corynebacterium glutamicum unidentified sequence involved in histidine biosynthesis, sequence X04960 trpA; trpB; trpC; trpD; Tryptophan operon Matsui, K. et al. "Complete nucleotide and deduced amino acid sequences of trpE; trpG; trpL the Brevibacterium Iactofermentum tryptophan operon," Nucleic Acids Res., 13-10114 (1986) X07563 lys A DAP decarboxylase (meso-diaminopimelate Yeh, P. et al. "Nucleic sequence of the lysA gene of Corynebacterium decarboxylase, EC 4.1.1.20) glutamicum and possible mechanisms for modulation of its expression," Mo.

Gen. Genet., 212(1):112-1 19 (1988) X 14234 EC 4.1.1.31 Phosphoenolpyruvate carboxylase Eikmanns, B.J. et al. "The Phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: Molecular cloning, nucleotide sequence, and .expression," Mol. Gen. Genet., 218(2):330-339 (1989); Lepiniec, L. et al.

"Sorghum Phosphoenolpyruvate, carboxylase gene family: structure, function molecular evolution," Plan. Mol. Biol., 21 (3):487-502 (1993) X 17313 fda Fructose-bisphosphate aldolase Von der Osten, C.H. et al. "Molecular cloning, nucleotide sequence and finestructural analysis of the Corynebacterium glutamicum fda gene: structural comparison of C. glutamicum fructose-I1, 6-biphosphate aldolase to class l and class 11 aldolases," Mo!. Microbiol., X53993 dapA L-2, 3-dihydrodipicolinate synthetase (EC Bonnassie, S. et al. "Nucleic sequence of the dapA gene from Corynebacterium glutam icum," Nucleic Acids Res., 18(21 ):642 1 (1990) X54223 AttB-related site Cianciotto, N. et al. "DNA sequence homology between att B3-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum and the attP site of lambdacorynephage," FEMS. Microbial, Lett., 66:299-302 (1990) X54740 argS; lysA Arginyl-tRNA synthetase; Diaminopimelate Marcel, T. et al. "Nucleotide sequence and organization of the upstream region decarboxylase of the Corynebacterium glutamicum lysA gene," M6. Microbial., 4(1li):18 19- 1 1830(1990) 2006200802 24 Feb 2006 2 (continued) X55994 trpL; trpE Putative leader peptide; anthranilate Heery, D.M. et al. "Nucleotide sequence of the Corynebacterium glutamicumn component I trpE gene," Nucleic Acids Res., 18(23):7138 (1990) X56037 thrC Threonine synthase Han, K.S. et al. "The molecular structure of the Corynebacterium glutamicum synthase gene," Mol. Microbiol., 4(10):1693-1702 (1990) X56075 atiB-related site Attachment site Cianciotto, N. et at. "DNA sequence homology between att B-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum and the attP site of lambdacorynephage," FEMS. Microbiol, 66:299-302 (1990) X57226 lysC-alpha; IysC-beta; Aspaztokinase-alpha subunit; Kalinowski, J. et al. "Genetic and biochemical analysis of the Aspartokinase asd Aspartokinase-beta subunit; aspartate beta from Corynebacterium glutamicum," M61. Microbiol., 5(5):1 197-1204 (1991); semialdehyde dehydrogenase Kalinowski, J. et al. "Aspartokinase genes lysC alpha and lysC beta overlap and are adjacent to the aspertate beta- sem ialdehyde dehydrogenase gene asd in ____________Corynebacterium glutamicum," Mol. Gen. Gene., 224(3):317-324 (1990) X59403 gap;pgk; tpi G lyceraldehyde-3 -phosphate; Eikmanns, B.J. "Identification, sequence analysis, and expression of a phosphoglycerate kinase; triosephosphate Corynebacterium glutaniicum gene cluster encoding the three glycolytic isomerase enzymes glyceraldehyde-3 -phosphate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomeras," J Bacteriol., 174(19):6076-6086 (1992) X59404 gdh Glutamnate dehydrogenase Bormann, et at. "Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase," McI. Microbial., 6(3):3 17-326 X60312 lys] L-lysine permease Seep-Feidhaus, et at. "Molecular analysis of the Corynebacterium glutamicum lysI gene involved in lysine uptake," McI. Microbial., 5(12):2995- (1991) X66078 cop I Psi protein Jolff, G. et at. "Cloning and nucleotide sequence of the cspl gene encoding PSI, one of the two major secreted proteins of Corynebacterium glutamicum: The deduced N-terminal region of PSI is similar to the Mycobacterium antigen complex," MoL. Microbiol., 6(16):2349-2362 (1992) X661 12 git Citrate synthase Eikmanns, B.J. et at. "Cloning sequence, expression and transcriptional analysis of the Corynebacterium glutamicumn gltA gene encoding citrate Microbial., 140:1817-1828 (1994) X67737 dapB Dihydrodipicolinate reductase X691 03 csp2 Surface layer protein PS2 Peyret, J.L. et al. "Characterization of the cspB gene encoding PS2, an ordered surface-layer protein in Corynebacterium glutamicum," Mol. Microbiol., (1993) X69104 153 related in'sertion element Bonamy, C. et al. "Identification of IS 1206, a Corynebacterium glutam icum 1S3-related insertion sequence and phylogenetic analysis," Mo!. MicrobioL, (1994) 2006200802 24 Feb 2006 Table 2 (continued) X70959 leuA Isopropylmalate synthase Patek, M. et "Leucine synthesis in Corynebacterium glutamicum: enzyme activities, structure of IeuA, and effect of leuA inactivation on lysine App!. Environ. Microbial., 60(l): 133-140 (1994) X71489 lcd Isocitrate dehydrogenase (NADP+) Eikmanns, B.J. et al. "Cloning sequence analysis, expression, and inactivation of the Corynebacterium glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme," J. Bacteriol, 177(3):774-782 (1995) X72855 GDHA Glutamate dehydrogenase (NA DPi-) X75083, mtrA 5-methyltryptophan resistance H-eery, D.M. et al. "A sequence from a tryptophan-hyperproducing strain of X70584 Corynebacterium glutamicum encoding resistance to Biochem. Biophys. Res. Commun., 201(3):1255-1262 (1994) X75085 recA Fitzpatrick, R. et al. "Construction and characterization of recA mutant strains of Corynebacterium glutamnicum and Brevibacterium lactofermentum," App!.

jot. Biolechnol, 42(4);575-580 (1994) X75504 aceA; thiX Partial Isocitrate lyase; Reinscheid, D.J. et al. "Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme," J.

176(1 2):3474-3483 (1994) X76875 ATPase beta-subunit Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit genes," Antonie Van Leeuwenhoek, 64:285-305 (1993) X77034 tuf Elongation factor Tu Ludwig, W. et al, "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit Antonie Van Leeuwenhoek 64:285-305 (1993) X77384 recA Billman-Jacobe, H. "Nucleotide sequence of a recA gene from glutamicum," DNA Seq., 4(6):403-404 (1994) X78491 aceB Malate synthase Reinscheid, D.J. et al. "Malate synthase from Corynebacterium glutamicum pta-ack operon encoding phosphotransacetylase: sequence analysis," Xicrobiology, 140:3099-3108 (1994) X80629 16S rDNA 16S ribosomal RNA Rainey, F.A. et al. "Phylogenetic analysis of the genera Rhodococcus and Norcardia and evidence for the evolutionary origin of the genus Norcardia frbm within the radiation of Rhodococcus species," Microbiol., 14 1:523-528 (1995) X81 191 gluA; gluB; gluC; Glutamnate uptake system Kronemeyer, W. et al. "Structure of the gluABCD cluster encoding the gluD glutamate uptake system of Corynebacterium glutamicum," J. BacierioL, 152-1158 (1995) X81379 dapE Succ inyldiaminopime late desuccinylase Wehrmann, A. et al. "Analysis of different DNA fragments of Corynebacterium glutamicum complementing dapE of Escherichia coli," 40:3349-56 (1994) 2006200802 24 Feb 2006 2 (continued) X82061 16S rDNA 16S ribosomal RNA Ruimy, R. et "Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences," Int. J Syst. Bacteriol., (1995) X82928 asd; lysC Aspartate-semialdehyde dehydrogenase; Serebrijski, 1. et al. "Multicopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," J 1 77(24):7255-7260 (1995) X82929 proA Gamma-glutamyl phosphate reductase Serebrijski, 1. et al. "Multicopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," J 1 77(24):7255-7260 (1995) X84257 16S rDNA 16S ribosomal RNA Pascual, C. et al. "Phylogenetic analysis of the genus Corynebacterium based 16S rRNA gene sequences," Int. J Syst. BacterioL,. 45(4):724-728 (1995) X85965 aroP; dapE Aromatic amino acid permease; Wehrmann et al. "Functional analysis of sequences adjacent to dapE of C.

glutamicum proline reveals the presence of aroP, which encodes the aromatic acid transporter," J. Bacteriol., 1 77(20):599 1-5993 (1995) X86 157 argfl; argC; argD; Acetylglutamate kinase; N-acetyl-gamma- Sakanyan, V. et al. "Genes and enzymes of the acetyl cycle of arginine argF; argJ glutamyl-phosphate reductase; biosynthesis in Corynebacterium glutamicum: enzyme evolution in the early acetylornithine aniinotransferase; omnithine steps of the arginine pathway," Microbiology, 142:99-108 (1996) carbamoyltransferase; glutamate Nacetyltransferase X89084 pta; ackA Phosphate acetyltransferase; acetate kinase Reinscheid, D.J. et al. "Cloning, sequence analysis, expression and inactivation of the Corynebacterium glutamicum pta-ack operon encoding ________________phosphotransacetylase and acetate kinase," Microbiology, 145:503-5 13 (1999) X89850 attB Attachment site Le Marrec, C. et al. "Genetic characterization of site-specific integration functions of phi AAU2 infecting "Arthrobacter aureus C70," J Bacteril., 178(7): 1996-2004 (1996) X90356 Promoter fragment Fl Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90357 Promoter fragment F2 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90358 Promoter fragment FIO Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142: 1297-1309 (1996) X90359 Promoter fragment F13 Patek, M. et "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) 2006200802 24 Feb 2006 2 (continued X90360 Promoter fragment F22 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90361 Promoter fragment F34 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90362 Promoter fragment F37 Patek, M. et al. "Promoters from C. glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90363 .Promoter fragment F45 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90364 Promoter fragment F64 Patek, M. et al. "Promoters from Corynebacterium glutarnicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90365 Promoter fragment F75 Patek, M. et al.' "Promoters from Corynebacteriumn glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90366 Promoter fragment PFI10 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90367 Promoter fragment PFI104 Patek, M. et al. "Promoters from Corynebacteriumn glutainicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90368 Promoter fragment PP 109 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology.

142:1297-1309 (1996) X93513 aint Ammonium transport system iee, R.M. et al. "Functional and genetic characterization of the (methyl) ammonium uptake carrier of Corynebacteriuin glutamicum," J1 Biol. Chem., 271(1 0):5398-5403 (1996) X93514 betP Glycine betaine transport system Peter, et al. "Isolation, characterization, and expression of the Corynebacterium glutamicum betP gene, encoding the transport system for the compatible solute glycine betaine," J. Bacteriol., I178(1 7):5229-5234 (1996) X95649 orf4 Patek, M. et al.* Identification and transcriptional analysis of the dapB-ORF2dapA-ORF4 operon of Corynebacterium glutamicum, encoding two enzymes involved in L-lysine synthesis," Biotechnol. Lett., 19:1113-1117 (1997) X96471 lysE; lysG Lysine exporter protein; Lysine export Vrjic, M. et al. "A new type of transporter with a new type of cellular regulator protein function: L-lysine export from Corynebacterium glutamnicum," Mo.

I Microbiol., 22(5):815-826 (1996) 2006200802 24 Feb 2006 Table 2 (continued) X96580 panB; panG; xylB 3-methyl-2-oxobutanoate Salim, H. et at. "D-pantothenate synthesis in Corynebacteriumn glutamicum and hydroxymethyltransferase; pantoate-beta- use of panBC and genes encoding L-valine synthesis for D-pantothenate alanine ligase; xylulokinase overproduction," App. Environ. Microbiol., 65(5):1973-1979 (1999) X96962 Insertion sequence IS 1207 and transposase X99289 Elongation factor P Ramos, A. et aL. "Cloning, sequencing and expression of the gene encoding elongation factor P in the amino-acid producer Brevibacterium lactofermentumn (Corynebacterium glutamicumn ATCC 13869)," Gene, 198:217-222 (1997) YOO 140 thrB Homoserine kinase Mateos, L.M. et al. "Nucleotide sequence of the homoserine kinase (thrB) gene the Brevibacterium lactofermentum," Nucleic Acids Res., 15(9):3922 (1987) Y00l151 ddh Meso-diaminopimelate D-dehydrogenase Ishino, S. et al. "Nucleotide sequence of the meso-diaminopimelate D- (EC 1.4.1.16) dehydrogenase gene from Corynebacteriumn glutamicum," Nucleic Acids Res., (1987) Y00476 thrA Homoserine dehydrogenase Mateos, L.M. et al. "Nucleotide sequence of the homoserine dehydrogenase (thrA) gene of the Brevibacteriumn lactofermnentum," Nucleic Acids Res., 15(24):10598 (1987) Y00546 horn; thrB Homoserine dehydrogenase; homoserine Peoples, O.P. et al. "Nucleotide sequence and fine structural analysis of the kinase Corynebacteriumn glutamicumn hom-thrB operon," Mo. lvicrobiol., 2(1):63-72 (1988) Y08964 murC; ftsQfdivD; ftsZ UPD-N-acetylmuramate-alanine ligase; Honrubia, M.P. et al. "Identification, characterization, and chromosomal division initiation protein or cell division organization of the ftsZ gene from Brevibacteriumn lactofermentum," Mol. Gen.

protein; cell division protein Gene., 259(1):97-104 (1998) Y09163 putP High affinity proline transport system Peter, H. et al. "Isolation of the putP gene of Corynebacterium glutamicumproline and characterization of a low-affinity uptake system for compatible solutes," Arch Microbial., 168(2): 143-151 (1997) Y09548 pyc Pyruvate carboxylase Peters- Wend isch, P.G. et al. "Pyruvate carboxylase from Corynebacteriumn glutamicum: characterization, expression and inactivation of the pyc gene," 144:915-927 (1998) Y09578 leuB 3-isopropylmalate dehydrogenase Patek, M. et al. "Analysis of the leuB gene from Corynebacteriumn App!. Microbiol. Biolechnol., 50(1):42-47 (1998) Y 12472 Attachment site bacteriophage Phi- 16 Moreau, S. et al. "Site-specific integration of corynephage Phi-I 16: The construction of an integration vector," Microbiol., 145:539-548 (1999) Y12537 proP Proline/ectoine uptake system protein Peter, H. et al. "Corynebacterium glutamicumn is equipped with four secondary carriers for compatible solutes: Identification, sequencing, and characterization of the proline/ectoine uptake system, ProP, and the ectoine/proline/glycine carrier, EctP," J. Bacteriol.. 180(22):6005-6012 (1998) 2006200802 24 Feb 2006 2 (continued) Y 13221 gInA Glutamine synthetase I Jakoby, M. et al. "Isolation of Corynebacterium glutamicum g~nA gene glutam ine synthetase FEMS Microbiol Left., 154(1):81-88 (1997) Y 16642 lpd Dihydrolipoamide dehydrogenase YI18059 Attachment site Corynephage 304L Moreau, S. et al. "Analysis of the integration functions of φ304L: An module among corynephages," Virology, 255(1): 150-159 (1999) Z21501 argS; lysA Arginyl-tRNA synthetase; diaminopimelate Oguiza, J.A. et al. "A gene encoding arginyl-tRNA synthetase is located in the decarboxylase (partial) upstream region of the lysA gene in Brevibacterium lactofermentum: Regulation of argS-lysA cluster expression by arginine," J.

1 75(22):7356-7362 (1993) Z21502 dapA; dapB Dihydrodipicolinate synthase; Pisabarro, A. et al. "A cluster of three genes (dapA, orf2, and dapB) of dihydrodipicolinate reductase Brevibacterium lactofernientum encodes dihydrodipicolinate reductase, and a third polypeptide of unknown function," J. Bacteriol, 1 75(9):2743-2749 Z29563 thrC Threonine synthase Malumbres, M. et al. "Analysis and expression of the thrC gene of the encoded synthase," App!. Environ. MicrobioL, 60(7)2209-2219 (1994) Z46753 16S rDNA Gene for 16S ribosomal RNA Z49822 sigA SigA sigma factor Oguiza, J.A. et al "Multiple sigma factor genes in Brevibacterium lactofernientum: Characterization of sigA and sigB," J Bacieriol., 178(2):550- (1996) Z49823 galE; dtxR Catalytic activity UDP-galactose 4- Oguiza, J.A. et al "The galE gene encoding the UDP-galactose 4-epimerase of epimerase; diphtheria toxin regulatory Brevibacterium lactofermnentum is coupled transcriptionally to the dmdR gene," Gene, 177:103-107 (1996) Z49824 orf I; sigB SigB sigma factor Oguiza, i.A. et al "Multiple sigma factor genes in Brevibacteriumn lactofiermentumn: Characterization of sigA and sigB," J. BacterioL, 178(2):550- (1996) Z66534 Transposase Correia, A. et al. "Cloning and characterization of an IS-like element present in the genome of Brevibacterium lactofermentumn ATCC 13869," Gene, (1996) 'A sequence for this gene was published in the indicated reference. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.

TABLE 3: Corynebacterium and Brevibacterium Strains Which May be Used in the Practice of the Invention Brevibacterium ammoniagenes 21054 Brevibacterium ammoniagenes 19350 Brevibacterium ammoniagenes 19351 Brevibacterium ammoniagenes 19352 Brevibacterium ammoniagenes 19353 Brevibacterium ammoniagenes 19354 Brevibacterium ammoniagenes 19355 Brevibacterium ammoniageries 19356 Brevibacterium ammoniagenes 21055 Brevibacterium ammoniagenes 21077 Brevibacterium ammoniagcries 21553 Brevibacterium ammoniagenes 21580 Brevibacterium ammoniagenes 39101 Brevibacterium butanicum 21196 Brevibacterium divaricatum 21792 P928 Brevibacterium flavum 21474 Brevibacterium flavum 21129 Brevibacterium flavum 21518 Brevibacterium flavum BI 1474 Brevibacterium flavum BI 11472 Brevibacterium flavum 21127 Brevibacterium flavum 21128 Brevibacterium flavum 21427 Brevibacterium flavum 21475 Brevibacterium flavum 21517 Brevibacterium fiavum 21528 Brevibacterium flavum 21529 Brevibacterium fiavum Bi 11477 Brevibacterium fiavum Bi 1478 Brevibacterium flavum 21127 Brevibacterium flavum BI 11474 Brevibacterium healii 15527 Brevibacierium ketoglutamicum 21004 Brevibacterium ketoglutamicum 21089 Brevibacterium Icetosoreductum 21914 Brevibacterium lactofermentum Brevibacterium lactofermentum -74 Brevibacterium lactofermentum 77 Brevibacterium lactofermentum 21798 Brevibacterium lactofermentum 21799 Brevibacterium lactofermentum 21800 Brevibacterium lactofermentum 21801 Brevibacterium lactofermentum BI 11470 Brevibacterium Iactoferrnentum B1 11471 89 Brvbctru latfnetr 21086 4 B~ ~GT S Brevibacterium lactofermentumn 2140 Brevibacterium lactofermentumn 2140 Brevibacterium lactofermentumn 31269 Brevibacterium linens 9174 Brevibacterium linens 19391 Brevibacterium linens 8377 Brevibacterium paraff inolyticum 11160 Brevibacterium Spec. 717.73 Brevibacterium Spec. Brevibacterium Spec. 14604 Brevibacterium Spec. 21860 Brevibacterium Spec. 21864 Brevibacterium Spec. 21865 Brevibacterium Spec. 21866 Brevibacterium Spec. 19240 Corynebacterium acetoacidophilum 21476 Corynebacterium acetoacidophilurn 13870 Corynebacterium acetoglutamnicum B] 1473 Corynebacterium acetoglutamnicumn BI 1475 Corynebacterium acetoglutamicum 15806 Corynebacterium acetoglutamnicumn 21491 Corynebacterium acetoglutamnicumn 31270 Corynebacteriumn acetophilum B3671 Corynebacterium ammoniagenes 6872 2399 Corynebacterium ammoniagenes 15511 Corynebacterium fujiokense 21496 Corynebacterium glutamnicum 14067 Corynebacterium glutamnicumn 39137 Corynebacterium glutamnicumn 21254 Corynebacteriurn glutamnicumn 21255 Corynebacterium glutamnicumn 31830 Corynebacterium glutamnicumn 13032 Corynebacterium glutamnicumn 14305 Corynebacterium glutamnicum 15455 Corynebacterium glutamnicumn 13058 Corynebacterium glutamnicumn 13059 Corynebactenium glutamicum 13060 Corynebacterium glutamnicumn 21492 Corynebacterium glutamicum 21513 Corynebacterium glutamnicumn 21526 Corynebacterium glutarnicum 21543 Corynebacterium glutamnicum 13287 Corynebacterium glutamicum 21851____ Corynebacteriurn glutamnicumn 21253 Corynebacterium glutamnicumn 21514 Corynebacterium glutamnicumn 21516 Corynebacteriuni glutamnicumn 21299 Corynebacterium glutamicum 21300 Corynebacterium glutamicum 39684 Corynebacterium glutamicum 21488 Corynebacterium glutamicum 21649 Corynebacterium glutamicum 21650 Corynebacterium glutamicum 19223 Corynebacterium glutamicum 13869 Corynebacterium glutamicum 21157 Corynebacterium glutamicum 21158 Corynebacterium glutamicum 21159 Corynebacterium glutamicum 21355____ Corynebacterium glutamicum 31808 Corynebacterium glutamicum 21674 Corynebacterium glutamicum 21562 Corynebacterium glutamicum 21563 Corynebacterium glutamicum 21564 Corynebacterium glutamicum 21565 Corynebacterium glutamicum 21566 Coryriebacterium glutamicum 21567 Corynebacterium glutamicum 21568 Corynebacterium glutamicum 21569 Corynebacterium glutamicum 21570 Coryriebacterium glutamicum 21571 Corynebacterium glutamicum 21572 Corynebacterium glutamicum 21573 Corynebacterium glutamicum 21579 Corynebacterium glutamicum 19049 Corynebacterium glutamicum 19050 Corynebacterium glutamicum 19051 Corynebacterium glutamicum 19052 Corynebacterium glutamicum 19053 Corynebacterium glutamicum 19054 Corynebacterium glutamicum 19055 Corynebacterium glutamicum 19056 Corynebacterium glutamicum 19057 Corynebacterium glutamicum 19058 Corynebacterium glutamicum 19059 Corynebacterium glutamicum 19060 Corynebacterium glutamicum 19185 Corynebacterium glutamicum 13286 Corynebacterium glutamicum 21515 Corynebacterium glutamicum 21527 Corynebacterium glutamicum 21544 Corynebacterium glutamicum 21492 Corynebacterium glutamicum B8183 Corynebacterium glutamicum B8 182 Corynebacterium Iglutamicum B 12416____ lCorynebacterium Iglutamicum IB 12417 -91- Corynebacterium glutamicum ___B12418 Corynebacterium glutamicum BI 11476 Corynebacterium glutamicum 21608 Corynebacterium liliumn P973 Corynebacterium nitrilophilus 21419 11594 Corynebacterium spec. Corynebacterium spec. _____P4446 Corynebacterium spec. 3 1088 Corynebacterium spec. 31089 Corynebacterium spec. 31090 Corynebacterium spec. 31090 Corynebacterium spec. 31090 Corynebacterium spec. 15954 20145 Corynebacterium spec. 21857 Corynebacterium spec. 21862 Corynebacterium spec. 21 863 ATCC: American Type Culture Collection, Rockville, MD, USA FERM: Fermnentation Research Institute, Chiba, Japan NRRL: ARS Culture Collection, Northern Regional Research Laboratory, Peoria, IL, USA CECT: Coleccion Espanola de Cultivos Tipo, Valencia, Spain NCIMB: National Collection of Industrial and Marine Bacteria Ltd., Aberdeen, UK CBS: Centraalbureau voor Schimmelcultures, Baarn, NL NCTC: National Collection of Type Cultures, London, UK DSIMZ: Deutsche Sammlung von Mikroorganismen und Zelikulturen, Braunschweig, Germany For reference see Sugawara, et al. (1993) World directory of collections of cultures of microorganisms: Bacteria, fuingi and yeasts 4 'h edn), World federation for culture collections world data center on microorganisms, Saimata, Japen.

2006200802 24 Feb 2006 Table 4: Alignment Results Length Accession Name of Genbank Hit ID length Genbank Hit Source of Genbank Hit

(NT)

rxa0OO26 1509 homology Date of (GAP) Deposit 38,003 10-DEC-1998 GBRO:MMHC31OM6 158405 AF109906 GBHTG2:AC007029- 119007 AC007029 GBHTG2:AC007029 119007 AC007029 Mus musculus MHC class III region RD gene, partial cds; Bf, C2, Mus musculus G9A, NG22, G9, HSP7O, HSP7O, HSC7Ot, and smRNP genes, complete cds: G7A gene, partial cds; and unknown genes.

Homo sapiens clone 0J0855F1 6, SEQUENCING IN PROGRESS Homo sapiens 1 unordered pieces.

Homo sapiens clone DJ0855F16, SEQUENCING IN PROGRESS Homo sapiens 1 unordered pieces.

37,943 37,943 7-Apr-99 7-Apr-99 rxa00072 rxaOOlll 1116 rxaOOll2 1314 rxaOOl33 936 GBBA1:SAUSIGA GBBA1:SC5B8 GBBA2:AE001767 GB-EST3:AU075536 GB-GSS9:AQ1 57585 GBG5514:A0510314 GBBA1:SC2G5 GB..EST7:W64291 GB-PR3AC005624 GB.BA2:AF1 24600 GB-BA1 :MTCY1 59 GB-BA1 :MT3DEHQ GBBA1:BLELONP GB.YL1 :SPA024C9 2748 M94370 Stigmatella aurantiaca sigma factor (sigA) gene, complete cds. Stigmatella aur 28500 AL022374 Streptomyces coelicolor cosmid 588. Streptomycesc 9086 AE001767 Thermotoga maritima section 79 of 136 of the complete genome. Thermotoga m~ 418 AU075536 AU075536 Rice shoot Oryza sativa cONA clone S0028 2Z, mRNA Oryza sativa sequence.

647 AQ157585 nbxbOOOSBl6r CUGI Rice BAC Library Oryza sativa genomic clone Oryza sativa nbxbOO0gBl6r, genomic survey sequenc.

542 AQ510314 nbxbOO95OO5f CUGI Rice BAC Library Oryza sativa genomic: clone Oryza sativa genomic survey sequence.

38404 AL035478 Streptomyces coelicolor cosmid 2G5. Streptomycesc 515 W64291 md98h12.rl Soares mouse embryo NbME13.5 14.5 Mus musculus Mus musculus cDNA clone IMAGE:386087 5' similar to gb:L26528 Mus musculus Rabi lb mRNA, complete cds (MOUSE);, mRNA sequence.

39594 AC005624 Homo sapiens chromosome 19, cosmid R30017, complete sequence. Homo sapiens antiaca ~oelicolor aritima 40,435 40,090 35,091 39,423 40,867 16-Aug-94 22-Apr-98 2-Jun-99 7-Jul-99 1 2-Sep-98 :oelicolor 39,372 04-MAY-1 999 41,170 11-Jun-99 35,306 10-Jun-96 a 39,054 6-Sep-95 99,867 04-MAY-i1999 rxaOl37 1212 4115 AF124600 Corynebacterium glutamicum chorismate synthase (aroC). shikimate kinase (aroK), and 3-dehydroquinate synthase (aroB) genes, complete cds: and putative cytoplasmic peptidase (pepO) gene, partial cds.

33818 Z83863 Mycobacterium tuberculosis H37Rv complete genome; segment 1111162.

3437 X59509 M.tuberculosis, genes for 3-dehydroquinate synthase and 3dehydroquinase.

738 X99289 B.lactofermentumn gene encoding elongation factor P.

38666 Z98601 S.pombe chromosome I cosmid c24C9.

Corynebacteriurn glutamnicum Mycobacterium tuberculosis Mycobacterium tuberculosis Corynebacterium glutamicumn Schizosaccharomyces pombe Caenorhabditis elegans 40,959 52,583 17-Jun-98 30-Jun-93 rxaOOl39 834 100,000 1-Nov-97 35.230 37,775 24-Feb-99 Z9971 1 GB-HTG1:CEY102A5_1110000 Z99711 Caenorhabdilis elegans chromosome V clone Y102A5, SEQUENCING IN PROGRESS in unordered pieces.

2006200802 24 Feb 2006 rxaG0152 1419 GBBAI:MTCY277 GBBA1:MSGY456 GB-BA2:AF0O2i 33 rxa00226 948 GB-PR3:AC005756 GBGSS5:AQ818463 GBGSS5:AQ782337 rxa1J0249 980 GB_8A2:AF035608 GB-BA1:ABO1 7641 GB-BA2:AF002133 Table 4 (continued) 38300 Z79701 Mycobacterium tuberculosis H37Rv complete genome; segment 65/162.

37316 ADOIOO01 Mycobacterium tuberculosis sequence from clone y456.

15437 AF002133 Mycobacterium avium strain GIRlO transcriptional regulator (may81) gene, partial cds, aconitase (acn), invasin 1 (invi), invasin 2 (inv2), transcriptional regulator (moxR), ketoacyl-reductase (fabG), enoylreductase (inhA) and ferrochelatase (may27?) genes, complete cds.

43299 AC005756 Homo sapiens chromosome 19, fosmid 39347, complete sequence.

413 AQ818463 HS 5250_A2_B88SP6E RPCI-11I Human Male BAC Library Homo sapiens genomic clone Plate=826 Col=16 Row=C, genomic survey sequence.

832 AQ782337 HS_-3184_81H12.37C CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3184 Col=23 Row=P, genomic survey sequence.

3614 AF035608 Pseudomonas aeruginosa ATP sulfurylase small subunit (cysD) and ATP sulfurylase GTP-bindling subunitlAPS kinase (cysN) genes, complete cds.

17101 AB017641 Micromonospora griseorubida gene for polyketidle synthase, complete cds.

15437 AF002133 Mycobacterium avium strain GIR10 transcriptional regulator (may81) gene, partial cds, aconitase (acn), invasin I (invl), invasin 2 (inv2), transcriptional regulator (moxR), ketoacyl-reductase (fabG), enoylreductase (inhA) and ferroctielatase (mav272) genes, complete cds.

2821 M89931 Coryriebacterium glutamicumn beta C-S lyase (aecD) and branchedchain amino acid uptake carrier (brnQ) genes, complete cds, and hypothetical protein Yhbw (yhbw) gene, partial cds.

2719 AJO01 436 Corynebacterium glutamicumn ectP gene.

5922 AF181035 Rhodobacter spliaeroides glycogen utilization operon, complete sequence.

3120 X56037 Corynebacterium glutamicumn thrC gene for threonine synthase (EC 4.2.99.2).

3146 109078 Sequence 4 from Patent WO 8809819.

73666 AL109954 Human DNA sequence from clone 333B1 5 on chromosome 20.

complete sequence.

885 X62977 D.ca rota mRNA for proliferating cell nuclear antigen (PCNA).

Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium avium Homo sapiens Homo sapiens Homo sapiens 58,500 38,913 64,009 36,209 37,288 35,917 17-Jun-98 03-DEC.1996 26-MAR-i 998 02-OCT-i1998 26-Aug-99 2-Aug-99 1-Jun-98 2-Apr-99 26-MAR-i1998 Pseudomonas aeruginosa 50.205 rxa00299 1101 rxaC0332 825 rxaOO47O 1392 GB-BA2:CORCSLYS GB-BA1 :CGECTP GB BA2:AF181035 GB BA1:CGTHRC GB PAT:109078 GB-PR3:HSJ333B15 GBPL2:DCPCNAM Micromonospora griseorubida Mycobacterium avium Corynebacterium glutamicum Corynebactenium glutamicum Rhodobacter sphaeroides Corynebactenium glutamicum Unknown.

Homo sapiens Daucus carota 40,266 38,429 100.000 4-Jun-98 41,143 36,701 37,730 38,700 37,203 37,914 20-Nov-98 7-Sep-99 17-Jun-97 02-DEC-i1994 23-Nov-99 30-Sep-99 2006200802 24 Feb 2006 GB-PL2:AC006267 101644 AC006267 Table 4 (continued) Arabidopsis thaliana BAC F9M1 3 from chromosome IV near 21.5 cM, Arabidopsis thaliana complete sequence.

Thermus thermophilus 1 OSa RNA gene. Thermus thermophilu S.erythraea first ORF of eryA gene, complete cds. Saccharopolyspora GB BA1:TT1OSARNA 721 GB-BAI:SERERYAA 11219 Y15063 M63676 Is rxa00471 813 GBPAT.:AR049367 GBBA1:SERERYAA 11219 AR049367 Sequence 1 from patent US 5824513.

11219 M63676 S.erythraea first ORF of eryA gene, complete cds.

erythraea Unknown.

Saccharopolyspora erythraea Honmo sapiens chromosome 19, cosmid R27370, complete sequence. Homo sapiens rxa00499 1404 GBPR4:AC007206 42732 AC007206 GB-EST26:A1344735 GBPR4:AC006479 rxa0O500 798 GBPR4:ACOO6111 GB-HTG2:AF128834 GBHTG2:AF128834 rxaOO501 630 GB-BA1:D86429 GB-HTGI :HS1099D15 GBHTG1:HSiO99D1S 462 A1344735 qp05al O.xl NCICGAPKid5 Homo sapiens cDNA clone IMAGE:19171 14 3 similar to gb:M15800 T-LYMPHOCYTE MATURATION-ASSOCIATED PROTEIN (HUMAN);, mRNA sequence.

161837 AC006479 Homo sapiens clone DJ1051J04, complete sequence.

190825 AC0061i11 Homo sapiens chromosome 16 clone RPCI-1 1-461 A8. complete sequence.

196589 AF128834 Homo sapiens chromosome 8 clone BAC 57G24 map 8 p1 2 SEQUENCING IN PROGRESS in unordered pieces.

196589 AF128834 Homo sapiens chromosome 8 clone SAC 57G24 map 8p1 2, SEQUENCING IN PROGRESS in unordered pieces.

5925 D86429 Saccharopolyspora rectivirgula gene for beta -galactosidase, complete cds.

1301 AL035456 Homo sapiens chromosome 20 clone RP5-1099D1 5, SEQUENCING IN PROGRESS in unordered pieces.

1301 AL035456 Homo sapiens chromosome 20 clone RP5-1099D1 5, SEQUENCING IN PROGRESS in unordered pieces.

42325 U00015 Mycobacterium Ieprae cosmid B1620.

36947 U00020 Mycobacterium leprae cosmid 8229.

210672 Z84464 Homo sapiens chromosome 13 clone 179115, -SEQUENCING IN PROGRESS in unordered pieces.

63033 AL021246 Mycobacterium tuberculosis H37Rv complete genome; segment 108/162.

2188 AF071885 Streptomyces coelicolor ATP-dependent dIp protease proteolytic subunit i'(clpPl) and ATP-dependent CIp protease proleolytic subunit 2 (clpP2) genes, complete cds; and ATP-dependent dIp protease ATP-binding subunit Clpx clpX) gene, partial cds.

15742 AF013216 Myxococcus xanthus Dog (dog), isocitrate lyase (ici), MIs (mls), Ufo (ufo), fumarate hydratase (Thy), and proteosome major subunit (clpP) genes, complete cds; and acyl-CoA oxidase (aco) gene, partial cds.

Homo sapiens Homo sapiens Homo sapiens Homo sapiens Homo sapiens Saccharopolyspora rectivirgula Homo sapiens Homo sapiens Mycobacterium Ieprae Mycobacterium leprae Homo sapiens Mycobacterium tuberculosis Streptomyces coelicolor Myxococcus xanthus 36,158 39.494 38,781 38,781 38,205 34,982 42,675 38,462 40,736 34,062 34,062 53,871 33.546 33,546 34,783 34,900 32,898 37,011 62,963 54,683 2-Feb-99 11 -Nov-99 3-Jul-99 28-Feb-99 28-Feb-99 '.0 -Is 09-DEC-i1998 23-Nov-99 23-Nov-99 01-MAR-i1994 01 -MAR-1 994 22-Jan-97 17-Jun-98 29-Jun-99 28-Jan-98 27-Apr-99 1 8-Aug-98 26-Apr-93 29-Sep-99 26-Apr-93 4-Apr-99 rxaOO5O2 1155 rxa00566 729 GB BA2:UOOO1 5 GBBA1:U.00020 GB-HTGI:HS179115 GBBA1:MTVO08 GBBA2:AF071885 GB BA2:AF013216 2006200802 24 Feb 2006 rxa00567 714 rxa00621 906 rxa00622 1539 rxa00650 759 rxa00675 915 rxa00689 1614 GB-BA1 :M1VOO8 GBBA1:CGBPHI16 GB-BA1 :ECOCLPPA GB-EST1 :036491 GB_1N2:CELC16A3 GBHTG3:AC00931 1 GBBAl ;AB004795 GBBA1:MBOPII GB_1N2:AF078916 GB-BA2:AF1 61327 GB-PL2:ATAC006533 GBPL2:ATAC005533 GBBAi:SC3C8 GBPR3:AC005736 GBIN2:AC00571 9 GB_-PAT:E07294 GB-BA1 BACALD-T GBBA2:PPU96338 63033 962 1236 360 34968 160198 3039 2392 2960 2021 99188 99188 33095 215441 188357 2975 1975 5276 AL021246 Y12472 J05534 D36491 U41534 AC00931 1 AB0D4795 D38405 AF07891 6 AF 161327 AC006533 AGO06533 AL023861 AC005736 AC005719 E07294 D1 3846 U96338 A16471 04 AA6361 59 Table 4 (continued) Mycobacterium tuberculosis H37Rv complete genome: segment 1081162.

C.glutamicum DNA, attachment site bacteriophage Phi-i 6.

Escherictiia call ATP-dependent cip protease proteolytic component (clpP) gene, complete cds.

CELK033GYF Yuji Kohara unpublished cDNA Caenorhabditis elegans cONA clone yk33gl 1 mRNA sequence.

Caenorhabditis elegans cosmid C16A3.

Homo sapiens clone NHO31 1 L03, **SEQUENCING IN PROGRESS 3 unordered pieces.

Pseudomonas sp. gene for dipeptdcyl aminopeptidlase, complete cds.

Moraxella lacunata gene for protease 11, complete cds.

Trypanosoma brucel brucei oligopeptidase B (opb) gene, complete ods.

Corynebacterium diphtheriae histidine kinase ChrS (chrS) and response regulator ChrA (chrA) genes, complete cds.

Arabidopsis thaliana chromosome 11 BAC F20M1 7 genomic sequence, complete sequence.

Arabidlopsis thaliana chromosome 11 BAC F2OM1 7 genomic sequence, complete sequence.

Streptomyces coelicolor cosmid 3C8.

Hiomo sapiens chromosome 16, BAC clone 462G18 (LANL), complete sequence.

Drosophila melanogaster, chromosome 2L, region 38A5-38B4, BAC clone BACR48MO5, complete sequence.

genomic DNA encoding dehydrogenase of Bacillus stearothermophilus.

B. stearothermophilus aldhT gene for aldehyde dlehydrogenase.

complete cdls. 4 Mycabacteriumn tuberculosis Corynebacterium glutamicum Eschenichia coli Caenorhabditis elegans Caenorhabditis elegans Homo sapiens Pseudomonas sp.

Moraxella lacunata Trypanosoma brucei brucei Corynebacterium diphtheriae Arabidlopsis Ihaliana Arabidopsis thaliana Streptomyces coelicolor Homo sapiens Drosophila melanogaster Bacillus stearothermophilus Bacillus stearothermophilus 42,090 40,000 52.119 40,390 35,477 38,636 54,721 50.167 48,076 51,319 38,051 35,403 36,836 42,027 35,531 45,677 45,677 44,317 58,511 41,195 17-Jun-98 05-MAR-i 999 26-Apr-93 8-Aug-94 18-MAY-i1999 13-Aug-99 5-Feb-99 8-Feb-99 08-OCT-i 999 9-Sep-99 26-MAY-i 999 26-MAY-i1999 15-Jan-99 01-OCT-i1998 27-OCT-i1999 29-Sep-97 20-Feb-99 13-MAY-i1999 29-Apr-99 22-OCT-i1997 Pseudomonas putidla NCIMB 9866 plasmid pRA4000 p-cresol Pseudomonas putidla dlegradlative pathway genes, p-hydroxybenzaldehyde dlehydrogenase (pchA), p-cresol methylhydroxylase, cytoc-hrome subunit precursor (pchC), unknown (pchX) and p-cresol methylhydroxylase, flavoprotein subunit (pchF) genes, complete cds.

vnl~cOi.yi Stratagene mouse heart (#937316) Mus musculus cONA Mus musculus done IMAGE: 1021248 mRNA sequence.

vnl5c0l.rl Stratagene mouse heart (#937316) Mus musculus cDNA Mus musculus dlone IMAGE:i10211248 mRNA sequence.

rxaGO715 918 GB-EST3:A647104 218 GB-EST1 7:AA6361 59 447 2006200802 24 Feb 2006 rxa00744 1065 rxaOO756 1119 rxa00773 1266 rxa00793 1299 rxa00820 486 rxa00833 618 rxa00844 957 GB-EST1 0:AA1 84468 GBHTG3:AC009855 GBHTG3:AC009855 GBPR4:AC005082 GB BA1 :MLCB596 GBGSS12:A0368028 GB HTG3:AC008067 GB BAI:MLU15182 GBBA1:MSGL611CS GBGSS14:AQ578181 GBGSS5:AQ769737 GB-BA1 :RTU08434 GBEST31:F33810 GBPR4:AC005868 GBEST8:PA000903 GB-EST25:A131 7789 GB PH:BPH6589 GBHTG2:AC006887 GBHTG2:AC006887 GB-GSS1 5:AQ6051 95 Table 4 (continued) 583 AA184468 mtS2hOS.rl Stratagene mouse embryonic carcinoma (#937317) Mus Mus musculus musculus cDNA clone IMAGE:633561 5' similar to gb:D1 0918 Mouse mRNA for ubiquitin like protein, partial sequence (MOUSE);. mRNA sequence.

167592 AC009855 Homo sapiens clone 1iC5, SEQUENCING IN PROGRESS ~,Homo sapiens 13 unordered pieces.

167592 AC009855 Homo sapiens clone 1_C_5, SEQUENCING IN PROGRESS ~.Homo sapiens 13 unordered pieces.

169739 AC005082 Homo sapiens clone RG271 G1 3, complete sequence. Homo sapiens 38426 AL035472 Mycobacterium leprae cosmid B596. Mycobacterium leprae 652 AQ368028 toxbOO0lNllr CUGl Tomato BAC Library Lycopersicon esculentumn Lycopersicon esculent genomic clone toxbOO01l Ni r, genomic survey sequence.

151242 AC008067 Homo sapiens clone NH-0303104, SEQUENCING IN PROGRESS Homo sapiens 2 unordered pieces.

40123 U15182 Mycobacterium Ieprae cosmid B2266. Mycobacterium leprae 37769 L78822 Mycobactedriu leprae cosmid 1611 D)NA sequence. Mycobacterium leprae 728 AQ5781 81 nbxbOO83PO~r CUGI Rice BAC Library Oryza sativa genomic clone Oryza sativa nbxbOO83PO8r, genomic survey sequence.

519 AQ769737 HS,3160_A2_G04_T7C CIT Approved Human Genomic Sperm Homo sapiens Library D Homo sapiens genomic clone Plate=3160 CoI=8 Row=M, genomic survey sequence.

2400 U08434 Rhizobiumn trifolii orotate phosphonibosyltransferase (pyrE) and Rhizobium trifolii fructokinase (frk) genes, complete cds.

243 F33810 HSPD27491 HM3 Homo sapiens cDNA clone s3000041 E12, mRNA Homo sapiens 36,673 3-Sep-99 36,673 3-Sep-99 urn 39,557 54,562 42,657 37,239 36,616 35,714 39,246 37,765 40,700 41,564 32,298 42,045 38,557 41,806 35,798 35,798 38,074 8-Sep-99 27-Aug-99 5-Feb-99 8-Sep-99 09-MAR-1995 15-Jun-96 2-Jun-99 28-Jul-99 16-Apr-97 13-MAY-1999 27-Feb-99 18-Jul-96 17-DEC-i1998 29-Apr-99 24-Feb-99 24-Feb-99 19-Jun-99 40,426 12-Feb-97 sequence.

96180 AC005868 Homo sapiens 12q24.2 PAC RPCI5-944M2 (Roswell Park Cancer Homo sapiens Institute Human PAC Library) complete sequence.

396 AA000903 mg38b04.rl Soares mouse embryo NbME1 3.5 14.5 Mus musculus Mus musculus cDNA clone IMAGE:426031 mRNA sequence.

696 A1317789 uj2O9.yl Sugano mouse embryo mewa Mus musculus cONA clone Mus musculus IMAGE:1 920544 5, similar to XNP:C1 3C4.5 CE081 30 SUGAR TRANSPORTER;, mRNA sequence.

41489 AJ006589 Bacteriophage phi-C31 complete genome. Bacteriophage 215801 AC006887 Caenorhabditis elegans clone Y59H1 1, SEQUENCING IN Caenorhabditis PROGRESS 3 unordered pieces.

215801 AC006887 Caenorhabditis elegans clone Y59H 11. SEQUENCING IN Caenorhabditis PROGRESS 3 unordered pieces.

459 A0605195 HS_2136_81_C1 2_T7C CIT Approved Human Genomic Sperm Homo sapiens Library D Homo sapiens genomic clone Plate=2136 Col=23 Row=F, genomic survey sequence.

phi-C31 elegans elegans 2006200802 24 Feb 2006 GBHTG1:CNSOOM8S 214599 GB-HTG1:CNSOOM8S 214599 GBBAi:CGORF4GEN 2398 Table 4 (continued) AL079302 Homo sapiens chromosome 14 done R-1089B7, SEQUENCING IN PROGRESS in ordered pieces.

AL079302 Homo sapiens chromosome 14 clone R-1 08987, ~*SEQUENCING IN PROGRESS in ordered pieces.

X95649 C.glutamicum ORF4 gene. rxa00866 1066 rxa00877 1788 rxaOO9O3 733 924 rxaOO906 627 rxa0O907 246 rxa00961 455 GBBA1:BLDAPAB GB-PAT:Ei4517 GB.,PAT:192050 GBPAT:178760 GB..BA2AE000426 GB-BA2AEOO1 598 GBPL2:AF079370 GBBA2:AE001598 GB-PR2:HSQ1 5024 GBGS54:AQ691923 GB-EST37:A1967802 GBPAT:178750 GBPAT:192039 GBPR3:HS929C8 GB..PAT:178750 GB PAT:192039 GBPAT:178750 GBBAl :AB032799 GSBBA2:AF172851 GB SAl :AB032799 3572 Z21 502 B. lactofermentum dapA and dapB genes for dihydrodipicolinate synthase and dlihydrodipicolinate reductase.

1411 E14517 DNA encoding Brevibacterium dihydrodipicolinic acid reductase.

567 192050 Sequence 17 from patent US 5726299.

567 178760 Sequence 16 from patent US 5693781.

10240 AE000426 Escherichia coli K-12 MG1655 section 316 of 400 of the complete genome.

11136 AE00 1598 Chiamnydia pneumoniae section 14 of 103 of the complete genome.

2897 AF079370 Kluyveromnyces lactis invertase (INVII) gene, complete cds.

11136 AE001 598 Chlamydlia pneumoniae section 14 of 103 of the complete genome.

73192 AJ239325 H-omo sapiens chromosome 21 from cosmids LLNLc1 16 10C16 and LLNLc1 16 15C24 map 21q22.3 region D21 Si 71 -LA1 61, complete sequence.

446 AQ691923 HS_5400_B2_G04_SP6E RPCI-1 1 Human Male BAC Library Homo sapiens genomic clone Plate=976 Col=8 Row=N. genomic survey sequence.

479 A1967802 Ljimpestl2-930-d6 Ijimp Lambda HybriZap two-hybrid library Lotus japonicus cDNA clone LP930-12-d6 5' similar to 60S ribosomal protein L7A, mRNA sequence.

588 178750 Sequence 6 from patent US 5693781.

588 192039 Sequence 6 from patent US 5726299.

139190 AL020994 Human DNA sequence from clone 929C8 on chromosome 22q12.1- 12.3 Contains CA repeat, GSS, STS, complete sequence.

588 178750 Sequence 6 from patent US 5693781.

588 192039 Sequence 6 from patent US 5726299.

588 178750 Sequence 6 from patent US 5693781.

9077 AB032799 Chromobacterium violaceum violacein biosynthetic gene duster (vio A, vio, B, vio C, vio complete cds.

10094 AF172851 Chromobacterium violaceumn violacein biosynthetic gene cluster, complete sequence.

9077 AB032799 Chromobacterium violaceum violacein biosynthetic gene cluster (vio A, vio, B, vio C, via complete cds.

Homo sapiens Homo sapiens Corynebacterium glutamicum Corynebacteriurn glutamicum Corynebacterium glutamicurn Unknown.

Unknown.

Escherichia coli Chlamydophila pneumnoniae Kluyveromyces lactis Chlamydophila pneumoruae Homo sapiens Homo sapiens Lotus japonicus Unknown.

Unknown.

Homo sapiens Unknown.

Unknown.

Unknown.

Chromobacterium violaceurn Chromobacterium violaceum Chromobacterium violaceum 38,120 38,120 99.273 99,301 99,659 62,787 62,787 36,456 15-OCT.1999 1 5-OCT-i1999 1 0-MAR-i 998 16-Aug-93 28-Jul-99 01-DEC-1998 3-Apr-98 12-Nov-98 32.782 08-MAR-i1999 35,849 40,138 35,076 33,500 41,127 97.071 97,071 39.016 97,561 97.561 37,222 39,868 42,760 39.551 4-Aug-99 08-MAR-i1999 28-Sep-99 6-Jul-99 24-Aug-99 3-Apr-98 01 -DEC-i1998 23-Nov-99 3-Apr-98 01-DEC-i1998 3-Apr-98 02-OCT-i1999 30-Aug-99 02-OCT-i1999 2006200802 24 Feb 2006 rxa00982 1629 rxa00983 1599 rxa00984 440 =0a1l14 2724 rxa01059 732 rxa0lO73 954 rxa01l2O 1401 GBBA1:BLARGS GB-BA1 :CGXLYSA GB-PATE14508 GB-HTG2:AC0081 52 GB-HTG2:ACOO8i 52 GBHTG3:AC008648 GB-BA1 :MVINED GBPAT:E02375 GBPR4:H-UAC004513 GBBA1:M1VOO8 GB BA1 :STMAMPEPN GBBA1:SC7H2 GBHTG3:AC008154 GBHTG3:AC008154 GB-EST32:A756574 GBBA1:BACOUTB GBPR4:AC007938 GBPL2:ATAC006282 GBBA1:MTVOO8 GB-BA1:CAJ1O321 2501 7-21501 2344 X54740 Table 4 (continued) BlIactofermentum argS and lysA genes far arginyl-tRNA synttietase Corynebacteriumn and diaminopimelate dlecarboxylase (partial). glutamicum Corynebactedriu glutamicum argS-lysA operon gene for the Corynebacteriumn upstream region of the arginyl-tRNA synthetase and diaminopimelate glutamicum decarboxylase (EC 4.1.1.20).

3579 24000 24000 87249 3098 1881 101311 63033 2849 42655 172241 172241 299 1004 167237 92577 63033 6710 E14508 AC0081 52 AC008152 AC008648 D01045 E02375 AC00451 3 AL021 246 L23172 AL1 09732 AC0081 54 AC0081 54 A1756574 M15811 AC007938 AC006282 AL021246 AJO1 0321 DNA encoding Brevibacterium diaminopimelic acid decarboxylase and arginyl-tRNA synthase.

Leishmania major chromosome 35 clone L7936 strain Friedlin, SEQUENCING IN PROGRESS 4 unordered pieces.

Leishmania major chromosome 35 clone L7936 strain Friedlin, SEQUENCING IN PROGRESS 4 unordered pieces.

Homo sapiens chromosome 5 clone CIT978SKB_-186E14, SEQUENCING IN PROGRESS 22 unordered pieces.

Micromonospora viridifaciens DNA for nedR protein and neuraminidase, complete cds.

Neuraminidase gene.

Homo sapiens Chromosome 16 BAC clone CIT987SK-A-926E7, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 1081162.

Streptomyces lividans aminopeptidase N gene, complete cds.

Streptomyces coelicolor cosmid 7H2.

Homo sapiens chromosome 7, -SEQUENCING IN PROGRESS 26 unordered pieces.

Homo sapiens chromosome 7, -SEQUENCING IN PROGRESS 26 unordered pieces.

eaO2flO.yl Eimeria M5-6 Merozoite stage Eimeria tenella cDNA 5', mRNA sequence.

Bacillus subtilis outB gene encoding a sporulation protein, complete cds.

lHoma sapiens clone UWGC:djs2Ol from 7q31, complete sequence.

Arabidopsis thaliana chromosome 11 BAC F13K3 genomic sequence, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 108/162.

Caulobacter crescentus partial tig gene and cIpP, cicA, clpX, Ion genes.

Corynebacterium glutamicum Leishmania major Leishmania major Hoamo sapiens Micromonospora viridifaciens Micromonospora viridifaciens Homo sapiens Mycobacteriurn tuberculosis Streptomyces lividans Streptomyces coelicolor A3(2) Hoamo sapiens Homo sapiens Eimeria tenella Bacillus subtilis Homo sapiens Arabidopsis thaliana Mycobacterium tuberculosis Caulobacter crescentus 39,003 41,435 40,566 38,658 38,658 36,102 59,226 59,226 41,204 56,167 57,067 37.551 39.499 39,499 37,793 53,723 34,322 36,181 36,715 63,311 28-DEC-i 993 30-Jun-93 28-Jul-99 28-Jul-99 28-Jul-99 3-Aug-99 2-Feb-99 29-Sep-97 23-Nov-99 17-Jun-98 18-MAY-i1994 2-Aug-99 8-Sep-99 8-Sep-99 23-Jun-99 26-Apr-93 1-Jul-99 13-MAR-1 999 17-Jun-98 01-OCT-i1998 2006200802 24 Feb 2006 rxa0ll47 1383 rxa01l151 958 GB-BA2:AF1 50957 GBPR3:HS408N23 GBBA2:AE001227 GB-PR3:HS408N23 GBBAl :MTCY261 GBHTG4:AC009849 GBHTG4:AC009849 GB-BA2:AF1 76799 G BBA2:AF012084 GB-EST32:A1728955 GBBA1:MLCB22 GB-BA1 :MTCY19O Table 4 (continued) 4440 AF1 50957 Azospirillum brasilense trigger factor (tig), heat-shock protein CIpP Azospirillumn brasilense (clpP). and heat-shock protein CIpX (clpX) genes, complete cds; and Lon protease (Ion) gene, partial cds.

97916 Z98048 Human DNA sequence from PAC 408N23 on chromosome 22q1 3. Homo sapiens Contains HIP, HSC70-INTERACTING PROTEIN (PROGESTERONE RECEPTOR-ASSOCIATED P48 PROTEIN), ESTs and STS.

26849 AE001227 Treponema pallidlum section 43 of 87 of the complete genome. Treponema pallidlum 97916 Z98048 Human DNA sequence from PAC 408N23 on chromosome 22q1 3. Homo sapiens Contains HIP, HSC70-INTERACTING PROTEIN (PROGESTERONE RECEPTOR-ASSOCIATED P48 PROTEIN), ESTs and STS.

60,613 34,567 37.564 34,911 7-Jun-99 23-Nov-99 16-Jul-98 23-Nov-99 nxa0ll6l 1260 27322 Z97559 Mycobacteriumn tuberculosis H37Rv complete genome; segment 95/162.

114993 AC009849 Drosophila melanog aster chromosome 2 clone BACRO7HO18 (0864) RPCI-98 07.H.8 map 31B-31C strain y; cn bw sp, SEQUENCING IN PROGRESS 55 unordered pieces.

114993 AC009849 Drosophila melanogaster chromosome 2 clone BACRO7HO8 (0864) RPCI-98 07.H-.8 map 31 B-31 C slrain y; cn bw sp, SEQUENCING IN PROGRESS 55 unordered pieces.

2943 AF176799 Lactobacillus pentosus PepO (pepO) and catabolite control protein A (ccpA) genes, complete cds.

3082 AF012084 Lactobacillus helveticus prolidlase (pepO) gene, complete cds.

611 A1728955 BNLGHi12114 Six-day Cotton fiber Gossypiumn hirsutumn cONA 5' similar to (AC004481) putative permease [Arabidopsis thalianal, mRNA sequence.

40281 Z98741 Mycobacterium leprae cosmid 822.

34150 Z70283 Mycobacterium tuberculosis H37Rv complete genome; segment 98/162.

40024 AL096872 Streptomyces coelicolor cosmid 5F7.

Mycobacteriumn 38,789 tuberculosis Drosophila melanogaster 39,213 Drosophila melanogaster 39,2 13 rxa0I 181 980 Lactobacillus pentosus Lactobacillus helveticus Gossypium hirsulumn Mycobacterium Ieprae Mycobacterium tuberculosis Streptomyces coelicolor A3(2) Caenorhabditis elegans Caenorhabditis elegans Caenorhabditis elegans Synechocystis sp.

37,043 46,796 37,647 61,570 60,434 57,011 34.843 34,843 34,843 36,538 17-Jun-98 25-OCT-1 999 25-OCT-i 999 5-Sep-99 1-Jul-98 11 -Jun-99 22-Aug-97 17-Jun-98 22-Jul-99 26-Oct-99 26-Oct-99 19-Nov-99 7-Feb-99 GB-BA1 :SC5F7 rxa0llB2 516 GB-HTG1:CEY116A8_2110000 Z98858 GBHTG1:CEY116A8_2110000 Z98858 GBIN1:CEY116A8C 260341 ALI117204 GB SAl :090915 130001 090915 GBBA1:D90915 130001 D90915 Caenorhabditis elegans chromosome IV clone Y1 16A8, SEQUENCING IN PROGRESS in unordered pieces.

Caenorhabditis elegans chromosome IV clone Y1 16A8.** SEQUENCING IN PROGRESS in unordered pieces.

Caenorhabditis elegans cosmid Y1 1 6A8C, complete sequence.

Synechocyslis sp. PCC6803 complete genome, 17/27, 2137259- 2267259.

Synechocystis sp. PCC6803 complete genome, 17/27. 2137259- 2267259.

rxa01l189 732 Synechocystis sp. 34,512 7-Feb-99 2006200802 24 Feb 2006 Table 4 (continued) GBHTG3AC0iO5i5 41038 AC010515 Homo sapiens chromosome i9.clone LLNL-R_-2491-9, SEQUENCING IN PROGRESS 31 unordered pieces.

GB_-OM:CFP18ORRC 5425 X87224 Canis familianis mRNA for ribosome receptor, p180.

GB-OM:CFP180RRC 5425 X87224 Canis familiaris mRNA for ribosome receptor. p18O.

rxa~li192 681 rxaOl2l4 1614 rxa01224 1146 rxa0125O 588 rxa01277 2127 Homo sapiens Canis familiaris Canis familiaris GBJN1 :CEY47D3A GB PR4:AC006039 GBPR4:AC006039 GBEST22:A1070047 GBRO:S75965 GB-EST5:H96951 GBPL1:NEULCCB

GBOV.:MTRACOMPL

GB-OV:AF090339 GBPL2:AF1 11709 GB INI1:CELZC25O GBEST1:Zi4808 GBBAi:MTCI65 GBBA1:MSGY348 GE1BAI :SC5C7 GBBA1:TAJ5O43 GB-PL2:ACO1 0675 199814 AL1 17202 Caenorhabditis elegans cosmid Y47D3A, complete sequence. Caenorhabditis elegans 176257 AC006039 Homo sapiens clone NHO319FO3, complete sequence. Homo sapiens 176257 AC006039 Homo sapiens clone NH0319F03, complete sequence. Homo sapiens 479 A1070047 UI-R-Cl-ln-f-08-0-UI.si UI-R-Ci Rattus norvegicus cDNA clone UI-R-Rattus norvegicus Ci-n-f-08-0-UI mRNA sequence.

625 S75 -965 THP=Tamm-HorsfalI protein (promoter) [rats, Genomic, 625 nt]. Rattus sp.

459 H96951 yu0lgO3.rl Soaresjpinealgland_-N3HPG Homo sapiens cDNA Homo sapiens clone IMAGE:232564 mRNA sequence.

2656 M18334 N.crassa (strain TS) laccase gene, complete cds. Neurospora crassa 16714 Y16884 Rhea americana complete mitochondrial genome. Mitochondrion Rhea 33,564 41,229 38,187 36,604 34,984 35,951 36,975 34,400 32,969 44.330 35,094 35,094 15-Sep-99 22-Jan-99 22-Jan-99 16704 AF090339 Rhea americana mitochondrion, complete genome.

52684 AF1 11709 Oryza saliva subsp. indica Retrosat 1 retrotransposon and Ty3- Gypsy type Retrosat 2 retrotransposon, complete sequences; and unknown genes.

34372 AF003383 Caenorhabditis elegans cosmid ZC250.

331 Z14808 CEL5E4 Chris Martin sorted cDNA library Caenorhabditis elegans cDNA clone cmn5e4 mRNA sequence.

34331 Z95584 Mycobacterium tuberculosis H37Rv complete genome; segment 50/162.

40056 AD000020 Mycobacteriumn tuberculosis sequence from clone y 3 48.

americana Mitochondrion Rhea americana Oryza sativa subsp. indica 37,410 rxa0l3O2 576 Caenorhabditis elegans Caenorhabditis elegans Mycobacterium tuberculosis Mycobacterium tuberculosis Streptomyces coelicolor Thermus thermophilus Arabidopsis thaliana rxa0l303 1458 41906 837 84723 AL031515 AJ225043 AC01 0675 Streptomyces coelicolor cosmid 5C7.

Thermus thermopliilus partial narl( gene.

Arabidopsis thaliana chromosome I BAC T171 3 genomic sequence, complete sequence.

35,506 36,890 59,298 59,227 39,261 55,.245' 37,058 38,610 55,445 36,815 54,942 62,423 19-Nov-99 05-MAY-i1999 05-MAY-i 999 5-Jul-99 27-Jul-95 11-DEC-i1995 03-MAY-i 994 i 9-Jul-99 27-MAY-1999 I 26-Apr-99 14-MAY-i 997 19-Jun-97 17-Jun-98 10-DEC-i1996 7-Sep-98 18-Jun-98 11 -Nov-99 17-OCT-i1998 7-Feb-99 29-MAY-i1997 7-Feb-99 24-Aug-99 GB-GSS9:AQ170862 518 rxa0l3O8 2503 rxa0i3O9 824 GB-BA1 :090757 GBBAl :D90787 GB BA1 :090758 GBBA1:SCJ12 17621 15942 13860 35302 AQ170862 HS -3165_-B2_-F03_-T7 CIT Approved Human Genomic Sperm Library Homo sapiens D Homo sapiens genomic clone Plate=3i65 Col=6 Row=L, genomic survey sequence.

090757 Escherichia coi genomic DNA. (27.3 27.7 min). Escherichia coli 090787 E.coli genomic DNA, Kohara done #276(33.0-33.3 min.). Escherichia coli 090758 Escherichia coli genomic DNA. (27.6 27.9 mi). Escherichia coli AL109989 Streptomyces coelicolor cosmid J1 2. Streptomyces coelicolor A3(2) GB-BA1:BSNARYWI 12450 GBBA1:SNARYW 12450 Z49884 B.subtilis nar[G,H,IJKJ, ywi[C,DEJ and argS genes. Bclu utls5.4 4Jn9 Bacillus subtilis 57,447 24-Jun-98 2006200802 24 Feb 2006 rxa01358 1644 rxa0l385 2004 GBBA1:BSUBOO2O GBGSS1i:AQ260413 GB-EST20:AA840582 GBPAT:A39944 GBBA1:FVBPENTA GBPAT: 119994 GB-BA2:AF059680 GBGSS12:AQ332469 GBEST27:AA998532 GBHTG1:HSA342D1 GB_ BA2:AE000745 GB-BA2:AE000745 Table 4 (continued) 212150 Z99123 Bacillus subtilis complete genome (section 20 of 21): from 3798401 to Bacillus subtilis 4010550.

453 AQ260413 CITBI-E1-2510B12.TF CITBI-El Homo sapiens genomic clone Homo sapiens 25101312, genomic survey sequence.

326 AA840582 vw77h07.rl Stratagene mouse heart (#937316) Mus musculus cDNA Mus musculus done IMAGE:i261021 5' similar to gb:J041 81 Mouse A-X actin mRNA, complete cds (MOUSE):, mRNA sequence.

3836 A39944 Sequence 1 from Patent W09421807. unidentified 2519 M98557 Flavobacteriumn sp. pentachlorophenol 4-monooxygenase gene, Flavobacterium complete mRNA.

2516 119994 Sequence 2 from patent US 5512478. Unknown.

2410 AF059680 Sphingomonas sp. UG30 pentachiorophenol 4-monooxygenase Sphingomonas (pcpB) gene, complete cds; and pentachlorophenol 4monooxygenase reductase (pcpD) gene, partial cds.

459 AQ332469 HS_-5003_-Al_-H08 _SP6E RPCI11I Human Male BAC Library Homo Homo sapiens sapiens genomic clone Plate=579 CoI=1 5 Row=O, genomic survey sequence.

453 AA998532 UI-R-C0-ic-d-1 1-0-Ul.si UI-R-CO Rattus norvegicus cDNA clone UI- Rattus norvegict R-CO-ic-d-1 1-0-Ul 3, mRNA sequence.

178183 AL1 21748 Homo sapiens chromosome 10 clone RP1 1-342D1i,1. Homo sapiens SEQUENCING IN PROGRESS in unordered pieces.

15085 AE000745 Aquifex aeolicus section 77 of 109 of the complete genome. Aquifex aeclicus 15085 AE000745 Aquifex aeolicus section 77 of 109 of the complete genome. Aquifex aeolicus sp.

sp. UG30 37,129 41,531 42,901 38.764 40,855 40.855 42,993 38,208 39,336 40,550 37,694 35,567 57,500 35,655 26-Nov-97 24-OCT-i1998 27-Feb-98 05-MAR-i1997 26-Apr-93 07-OCT-i1996 27-Apr-99 06-MAR-i1999 09-MAR-i1999 23-Nov.99 25-MAR-i1998 25-MAR-i1998 rxa01412 327 us rxa01458 1173 rxa0lS7l 723 nxa01607 753 GB BA1:ABO1 1413 12070 ABO1 1413 GB-BA1:AB01 1413 12070 AB301 1413 GB -PR4:AC005005 133893 AC005005 GBHTG3:AC008257 109187 AC008257 GBHTG3:AC008257 109187 AC008257 Streptomyces griseus genes for Orf2, Orf3, Orf4, OrfS, AfsA, Orf8, partial and complete cds.

Streptomyces griseus genes for Orf2, Orf3, 0rf4, OrfS, AfsA, Orf8, partial and complete cds.

Streptomyces griseus Streptomyces griseus 7-Aug-98 7-Aug-98 Homo sapiens PAC clone DJ412 'A9 from 22, complete sequence. Homo sapiens Drosophila melanogaster chromosome 2 clone BACR08AI 1 (D916) Drosophila melanogaster RPCI-98 08.A.1 1 map 42A-42A strain y; cn bw sp, **SEQUENCING IN PROGRESS 93 unordered pieces.

Drosophila melanogaster chiromosome 2 clone BACROWA 1 (0916) Drosophila melanogaster RPCI-98 08.A.1 1 map 42A-42A strain y; cn bw sp, **SEQUENCING IN PROGRESS 93 unordered pieces.

Mycobacteriumn tuberculosis H37Rv complete genome; segment Mycobacterium 125/162. tuberculosis Mycobacteriumn leprae cosmid B1529 DNA sequence. Mycobacterium leprae Pseudomonas aeruginosa dapD) gene for tetra hydrodipico linate N- Pseudomonas aeruginosa succinyletransferase, complete cds, strain PAOi.

38341 33,741 39,369 60,624 41,603 02-MAR-i1999 08-OCT-i1999 08-OCT-i 999 17-Jun-98 15-Jun-96 12-MAR-1 999 rxa01609 996 GB-BA1 :MTVOO3 13246 AL008883 GBBA1:MSGB1529CS 36985 L78824 GB-BA1 :AB024601 14807 AB024601 2006200802 24 Feb 2006 rxaOi654 1119 GB-GSS4:AQ704352 GBRO:MMAEOOO663 GB-EST23:A1158428 GB-OV:AF0261 98 rxa01664 945 rxa01795 720 rxa0l8O2 954 rxa01838 842 rxa0i848 867 GBPR3:AC004466 GBPR3:AC004466 GBBA2:CGU13922 GB BAi:SB6113 GB PAT:122080 GB-BA2:AE0015i9 GB-GSS5:AQ774071 GBPR4.AC007459 GB BA1 :SCEi5 GB-HTG3:AC009545 GBHTG3:AC009545 OB-BAl :MTCY24A1 GB_EST21:C89252 GBEST14:AA423340 Table 4 (continued) 532 AQ704352 HS_2147_A2_H04_MR CIT Approved Human Genomic Sperm Library D Homo sapiens genomic: clone Plate=2i47 Col=8 Row=O, genomic: survey sequence.

250611 AE000663 Mus musculus TCR beta locus from bases 1 to 250611 (section 1 of 3) of the complete sequence.

511 A1158428 ud24f1 2.rl Soares 2NbMT Mus musculus cDNA clone IMAGE:1446863 mRNA sequence.

63155 AF026198 Fugu rubripes neural cell adhesion molecule Li homolog (Li1-CAM) gene, complete cds; putative protein 1 (PUll) gene, partial cds; mitosis-specific chromosome segregation protein SMC 1 homolog (SMVCl1) gene, complete cds; and calcium channel alpha-i subunit homolog (CCA1) and putative protein 2 (PUT2) genes, partial cdls, complete sequence.

122186 AC004466 Homo sapiens 12q13.1 PAC RPC15-1057120 (Roswell Park Cancer Institute Human PAC library) complete sequence.

122186 AC004466 Homo sapiens 12q13.1 PAC RPC15-1 057120 (Roswell Park Cancer Institute Human PAC library) complete sequence.

4412 U13922 Corynebacterium glutamicum putative type 11 5-cytosoine methyltransferase (cgllM) and putative type 11 restriction endlonuclease (cgllR) and putative type I or type Ill restriction endonuclease (dgllR) genes, complete cds.

1044 S86113 ORF 1 INeisseria gonorrhoeae. Genomic, 1044 ntJ.

850 122080 Sequence 1 from patent US 5525717.

14062 AE001519 Helicobacter pylori, strain J99 section 80 of 132 of the complete genome.

552 AQ774071 HS_2269_21_ClO_-T7C CIT Approved Human Genomic Sperm Library D Hlomo sapiens genomnic clone Plate=2269 Col=19 Row=F, genomic survey sequence.

40907 AC007459 Homo sapiens chromosome 16 clone 306C6, complete sequence.

26440 AL049707 Streptomyces coelicolor cosmid ElI5.

165042 AC009545 Homo sapiens chromosome 11 clone 131 -J 04 map 11, SEQUENCING IN PROGRESS 8 unordered pieces.

165042 AC009545 Homo sapiens chromosome 11 clone 131 -J 04 map 11, SEQUENCING IN PROGRESS 8 unordered pieces.

20270 Z95207 ,Mycobacterium tuberculosis I-37Rv complete genome: segment 124/162.

587 C89252 C89252 Mouse early blastocyst cODNA Mus musculus cONA clone O1BOOO61JCO8, mRNA sequence.

457 AA423340 ve39d04.rl Soares mouse mammary gland NbMMG Mus musculus c:DNA clone IMAGE:820519 mRNA sequence.

Homo sapiens Mus musculus Mus musculus Fugu rubripes Homo sapiens Homo sapiens Corynebacteium glutamicum Neisseria gonorrhoeae Unknown.

Helicobacter pylori J99 Homo sapiens Homo sapiens Streptomyces coelicolor Homo sapiens Homo sapiens Mycobacterium tuberculosis Mus musculus Mus musculus 37,838 35,799 41,337 35,187 37,382 37.325 99,444 58.320 57,722 33,510 37,967 39,140 36,297 37.651 37,651 38,270 37,219 7-Jul-99 4-Sep-97 30-Sep-98 02-MAY-i1998 17-Sep-98 17-Sep-98 3-Feb-98 07-MAY-i1993 07-OCT-i1996 20-Jan-99 29-Jul-99 04-MAY-i1999 22-Apr-99 01-OCT-1999 01-OCT-i 999 17-Jun-98 28-MAY-i1998 38.377 16-OCT-1997 2006200802 24 Feb 2006 rxaD1849 1224 rxa01868 2049 rxa01885 924 rxa0l9l4 526 rxa01932 1020 nca01933 726 rxa0l97l 954 GBBA1 :MTCY24A1 GB_ BA2:RCP-SYNG GB_BA1:RSPO1O3O2 GB-BA1 :M1V033 GBBAI:MLCL622 GB..BA1 :MSGB983CS GBBA1:MTCY1A1O GB-PR3:HSU22OBi 1 GBBA1:PDU17435 GB-PR3:AC005796 GBPR3:HS39OC10 GB-PR3:AC005796 GBPR3:AC003025 GBGSS3:B78728 GBPR3:AC003025 GBHTG1:HS74016 GBHTGI:HS74016 GB-PR4:AC006032 GB_HTG3:ACJO823O GB-HTG3:AC008230 20270 45959 40707 21620 42498 36788 25949 41247 993 43843 114231 43843 112309 312 112309 169401 169401 170282 108469 Z95207 Z1i1165 AJO10302 AL021 928 Z95398 L78828 Z95387 Z69908 U 17435 AC005796 AL008721 AC005796 AC003025 B78728 AC003025 AL110119 AL110119 AC006032 AC008230 Table 4 fcontinued) Mycobacterium tuberculosis H37Rv complete genome; segment Mycobacteriumn 1241162. tuberculosis R.capsulatus comrplete photosynthesis gene cluster. Rhodobacter capsulatus Rhodobacter sphaeroides photosynthetic gene cluster. Rhodlobacter sphaeroides Mycobacterium tuberculosis H37Rv complete genome; segment Mycobacteriumn 11/162. tuberculosis Mycobacteriumn Ieprae cosmid L622. Mycobactenumr leprae Mycobacterium leprae cosmid 8983 DNA sequence. -Mycobacteriumn Ieprae Mycobacterium tuberculosis H37Rv complete genome; segment Mycobacterium 117/1 62. tuberculosis Human DNA sequence from cosmid cU22OB1 1, between markers Homo sapiens DXS6791 and DXS8038 on chromosome X.

Paracoccus denitrificans Fnr-like transcriptional activator (nnr) gene, Paracoccus. denitrificans complete cdls.

Homo sapiens chromosome 19, cosmid R31 408, complete sequence. Homo sapiens Homo sapiens DNA sequence from BAC 390010 on chromosome Homo sapiens 22ql 1.21-12.1. Contains an Immunoglobulin LIKE gene and a pseudlogene similar to Beta Crystallin. Contains ESTs. STSs, GSSs and taga and tat repeat polymnorphisms, complete sequence.

Homo sapiens chromosome 19, cosmid R31408, complete sequence. Homo sapiens Human Chromosome 11lpl 2.2 PAC clone pDJ466al11. complete Homo sapiens sequence.

CIT-HSP-431 E3.1V CIT-HSP Homo sapiens genomic clone 431 E3. Homo sapiens genomic survey sequence.

Human Chromosome 11lpl 2.2 PAC dlone pDJ466all1, complete Homo sapiens sequence.

Homo sapiens chromosome 21 clone RPCIP70.401674 map 21q21, Home sapiens **SEQUENCING IN PROGRESS *,in unordered pieces.

Homo sapiens chromosome 21 clone RPCIP70401674 map 21q21, Home sapiens **SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens BAC clone NHO1 15E20 from Y. complete sequence. Home sapiens 39.950 37,344 40,898 38,679 38,911 38,933 51 .094 39,038 39.390 34,961 39,600 37,725 35,585 38,907 35,859 35,302 35,302 37.640 35,466 17-Jun-98 2-Sep-99 27-Aug-99 17-Jun-98 24-Jun-97 15-Jun-96 17-Jun-98 23-Nov-99 19-Jul-95 06-OCT-i 998 23-Nov-99 06-OCT-i 998 23-Jul-98 25-Jun-98 23-Jul-98 27-Aug-99 27-Aug-99 27-Feb-99 10-Aug-99 Drosophila melanagaster chromosome 2 clone BACR 17117 (0934) RPCI-98 17.1.17 map 53A-53C strain y; on bw sp, -SEQUENCING IN PROGRESS 108 unordered pieces.

Drosophila melanogaster chromosome 2 clone BACR17117 (0934) RPCI-98 17.1.17 map 53A-53C strain y; cn bw sp, SEQUENCING IN PROGRESS-, 108 unordered pieces.

Drosophila melanogaster 108469 AC008230 Drosophila melanogaster 35,466 10-Aug-99 2006200802 24 Feb 2006 GB-PR3:AFOE64860 Table 4 (continued) 165382 AF064860 Homo sapiens chromosome 21q22.3 PAC 70124, complete sequence.

Homo sapiens rxa02016 900 GBEST2:D48846 GB-GSS1 0:AQ195886 GBGSSIO:AQ195886 rxaO2O1 7 807 GB-EST2O:AA85526 GBEST2O:AA855266 rxa02018 1073 GBBAl:SC5C7 GB-BA1:SCJ12 rxa02048 1497 GBPAT:E15823 GB OMSSAMPTDN GB-OVD87992, rxaO02I01 1386 GB BAI :AP000054 GB-PL2:ATAC006587 GB-PL2:ATAC006587 rxa02265 423 GB BA2:AF120718 GBPAT:E03531 GBBA1:LBAAURE rxa02276 801 GB-GSS1O:AQ242920 41906 34331 35302 2323 3387 3181 247695 79262 79262 4137 2896 2896 451 14503 5542 D48846 AQ1 95886 AQ195886 AA855266 AA855266 AL031515 Z95584 AL109989 El15823 Z29522 D87992 AP000064 AC006587 AC006587 AF120718 E03531 D1 0605 AQ242920 D29637 RICS1 5292A Rice green shoot Oryza sativa cONA, mRNA 'Oryza sativa sequence.

RPCI1I 1-66013.TJ RPCI-i1 1 Homo sapiens genomic clone RPCI-1 1I-* Homo sapiens 66013, genomic survey sequence.

RPCI1 1-66013.TJ RPCI-1 1 llama sapiens genomic clone RPCI-11 1- Homo sapiens 66013, genomic survey sequence.

vw7ObO8.rl Stratagene mouse heart (#937316) Mus musculus cDNA Mus musculus clone IMAGE:1260279 mRNA sequence.

vw7ObO8.rl Stratagene mouse heart (#937316) Mus musculus cONA Mus musculus clone IMAGE:i260279 5F, mRNA sequence.

Streptomyces coelicolor casmid 5C7.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 50/162.

Streptomyces coelicolor cosmid J1 2.

DNA encoding cell surface protein from Corynebacteriumn ammoniagenes.

S.scrofa mRNA for aminopeptidase N.

Gallus gallus mRNA for aminopepfidase Ey, complete cds.

Aeropyrumn pernix genomic DNA, section 7M.

Arabidlopsis thaliana chromosome 11 BAC Ti 7012 genomic sequence, complete sequence.

Arabidlopsis thaliana chromosome I I BAC TI 7D1 2 genomic sequence, complete sequence.

Lactobacillus fermentumn u rease operon. partial sequence.

DNA sequence coding for acid urease.

L-fermentumn gene for acid urease.

HS_2061_AlE08_MR CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2061 Col=15 Row=l, genomic; survey sequence.

Physarumn polycephalumn mitochondrial DNA.

Streptomyces coelicolor Mycobacteriumn tuberculosis Streptomyces coelicolor A3(2) Corynebacteriumn ammoniagenes Sus scrofa Gallus gallus Aeropyrum pernix Arabidopsis thaliana Arabidopsis thaliana Lactobacillus fermentumn Lactobacillus fermentumn Lactobacillus fermentumn Homo sapiens Mitochondrion Physarumn polycephalumn 39,716 37,118 41,000 34,790 42,638 37,183 41,732 62.395 61603 53,942 42,672 41,554 39,882 38,490 34,863 56,265 56,265 56,265 37,916 40,335 2-Jun-98 2-Aug-95 20-Apr-99 20-Apr-99 06-MAR-i 998 06-MAR-1998 7-Sep-98 17-Jun-98 24-Aug-99 28-Jul-99 26-Sep-94 5-Jun-99 22-Jun-99 23-MAR-i1999 23-MAR-i1999 31-MAR-i1999 29-Sep-97 2-Feb-99 03-OCT-i1998 12-MAY-1999 08-MAY-i 998 9-Feb-99 GBJIN1 :SLMMTPMF GBJIN2:AF012249 AF012249 Physarum polycephalum strain aux2-S region of mitochondria derived Mitochondrion Physarum 40,335 from mF plasmid, including URFA', URFC, URFO. URFE, URFF, and polycephalumn URFG genes, complete cds, and URFIH gene, partial cdls.

AF048784 Actinomyces naeslundii urease accessory protein (ureG) gene, Actinomyces naeslundii 66,814 complete cds.

rxa02277 738 GB-BA2:AF048784 681 2006200802 24 Feb 2006 GBBA2:AF056321 rxa02278 972 rxa0J2317 735 rxa02334 746 GBBA2:SSU35248 GB-GSS3:B49054 GB PL1 :PMCMSGI GBPL2:AF038556 GB-GSS8:A0051031 GBGSS8:AQ051031 GBBA1:CGU35023 GB-BA1 :MTCY71 GBBA1:U00012 GBHTG2:H5225E12 GBHTG2:HS225E12 GBHTG2:HS225E12 GBBA1 :AB020624 GB-EST4:H51 527 GB-GSS1 :CNS0O3CM Table 4 (continued) 5482 AF056321 Actinomyces naeslundii urease gamma subunit UreA (ureA), urease Actinomyces naeslundii 63,686 beta subunit UreB (ureB), urease alpha subunit UreC (ureC), urease accessory protein UreE (ureE). urease accessory protein UreF (ureF), urease accessory protein UreG (ureG), and urease accessory protein UreD (ureD) genes, complete cdls.

5773 U35248 Streptococcus salivanius ure cluster nickel transporter homolog (urel) Streptococcus salivarius 61,931 gene, partial cds, and urease beta subunit (ureA), gamma subunit (ureB), alpha subunit (ureC), and accessory proteins (ureE), (ureF), (ureG), and (ureD) genes, complete cds.

543 B49054 RPCIi 1-4113.TV RPCI-1i1 Homo sapiens genomic clone RPCI-i 1- Homo sapiens 39,161 4113, genomnic survey sequence.

3363 L27092 Pneumocystis carinii B-cell receptor (msglI) gene, 3' end. Pneumocystis carinii 39.819 12792 AF038556 Pneumocystis carinji f. sp. hominis variant regions of major surface Pneumocystis carinii f. sp. 33,832 glycoproteins (msgl, msg3, msg4) genes, partial cdls. hominis 914 AQ051031 nbxbOOO4dGl Or CUGI Rice BAC Library Oryza sativa genomic clone Oryza sativa 32,299 nbxbOOO4N2Or genomic survey sequence.

914 AQ051031 nbxbOOO4dGl Or CUGI Rice BAC Library Oryza sativa genomic clone Oryza sativa 34,573 nbxbOOO4N2Or, genomic survey sequence.

26-Jan-96 8-Apr-99 26-Sep-94 10-Sep-98

C>

24-MAR-1999 t- 24-MAR-1 999 9-Feb-99 3195 U35023 Corynebacterium glutamicum thiosulfate sulfurtransferase (thtR) Corynebacterium gene, partial cds. acyl CoA carboxylase (accBC) gene, complete cds. glutamicum 100,000 16-Jan-97 rxa02351 1039 42729 Z92771 Mycobacteriumn tuberculosis H37Rv complete genome; segment 141/162.

33312 U00012 Mycobacterium leprae cosmid 131308.

126464 AL031772 Homo sapiens chromosome 6 clone RP1-225E12 map q24, SEQUENCING IN PROGRESS in unordered pieces.

126464 AL031772 Homo sapiens chromosome 6 clone RP1-225E12 map q24, SEQUENCING IN PROGRESS in unordered pieces.

126464 AL031772 Homo sapiens chromosome 6 clone RP1-225E12 map q24, SEQUENCING IN PROGRESS in unordered pieces.

1605 AB020624 Corynebacterium glutamicum murl gene for D-glutamate racemase, complete cds.

294 H51527 yo33b09.sl Soares adult brain N2b4HB55Y Homo sapiens cODNA clone IMAGE:179705 mRNA sequence.

1101 AL064136 Drosophila melanogaster genome survey sequence T7 end of BAC BACRO8C19 of RPCI-98 library from Drosophila melanogaster (fruit fly), genomic survey sequence.

Mycobacterium tuberculosis Mycobacteium leprae Hlomo sapiens H-omo sapiens Homo sapiens Corynebacerium glutamicum Homo sapiens 60,380 37,660 35,973 35,973 36,992 99,227 40,411 10O-Feb-99 30-Jan-96 03-DEC-i1999 03-DEC-i1999 03-DEC-i 999 24-Jul-99 18-Sep-95 3-Jun-99 rxaO2410 789 Drosophila melanogaster 37,674 2006200802 24 Feb 2006 rxa02477 744 GBHTG4:AC010054 130191 AC010054 GBHTG4:AC010054 130191 AC010054 GBHTG4:AC009375 137069 AC009375 Table 4 (continued) Drosophila melanogaster chromosome 3L/74E2 clone RPC198- -SEQUENCING IN PROGRESS 70 unordered pieces.

Drosophila melanogaster chromosome 3L174E2 clone RPC198- **SEQUENCING IN PROGRESS 70 unordered pieces.

Drosophila melanogaster chromosome 3L/75A1 clone RPCI98- 44L-18, SEQUENCING IN PROGRESS 59 unordered pieces.

Drosophila melanogaster 37,466 Drosophila melanogaster 37,466 Drosophila melanogaster 39,118 rxa02513 832 GB BA1 :MTER26O GBPL1:AB019229 GBPL1:A5019229 GBBAi:CGLAUTB 373 X92572 M.terrae gene for 32 k~a protein (partial).

84294 AB019229 Arabidopsis thaliana genomic DNA, chromosome 3. PI clone: MDC16, complete sequence, 84294 AB019229 Arabidopsis thaliana genomic DNA, chromosome 3, P1 clone: MDC16, complete sequence.

Mycobacteriumn terrae Arabidlopsis thaliana Arabidlopsis thaliana 42,895 36,084 35,244 1 6-OCT-1999 16-OCT-i1999 16-OCT-1 999 15-Jan-98 20-Nov-99 20-Nov-99 8-Aug-96 1 2-MAR-i 997 17-DEC-i1998 27-MAY-1999 rxa02531 834 271 GBEST1i:AA239557 423 GBBA1:RSPYPPCL 6500 G13 BA2:AF127374 63734 rxa02548 314 X89850 C.glutamicum DNA for attB region. Corynebacterium 40,590 glutamicum AA239557 mv25f04.rl GuayWoodford Beier mouse kidney day 0 M us musculus Mus musculus 38,760 cDNA done IMAGE:656095 5' similar to gb:X52634 Murine tfm oncogene for tim protein (MOUSE);, mRNA sequence.

AJ002398 Rhodobacter sphaeroides pyp and pci genes, and orfA, orfB, orfC, Rhodobacter sphaeroides 37,091 orfD, orfE, orfF.

AF127374 Streptomyces lavendlulae LinA homolog, cytochrome P450 Streptomyces lavendulae 66,242 hydroXylase ORF4, cytochrome P450 hydroxylase QRF3, MitT (mitT), MitS (mitS), MAtR (mitR), MitO (mitQ), MitP (mitP), MitO (mitO), MitN (mitN), MitM (mitM), MitL- (mitt.), MitK (mitK), MitJ (mitJl), Mitd (miti). MitH (mitH), MitG (mitG), MitlF (mitf), MitE (mitE), MitlD (mitD), MitC (mitC), MitB (mitB), MitA (mitA). MmcA (mmcA), MmcB (mmcB), MmcC (mmcC), MmcD (mmcD), MincE (mincE), MmcF (mmcF), MmcG (mmcG), MincH (mmcH), Mmcl (mmcl), MmcJ (inmcJ), MincK (mmcK), Mmcl- (mmcL). MmcM (mmcM), MmcN (inmcN), MincO (mmcO), Mrd (mrd), MmcP (mmcP), MmcQ (mmcQ), MmcR (mincR), MmcS (mmcS), MmcT (inmcT), MmcU (mincU), MmcV (mmcV), Mct (mct), MmcW (mmcW), MincX (mincX), and MincY (mincY) genes, complete cds; and unknown genes.

2006200802 24 Feb 2006 GBBA2:AF127374 GB-GSS4:AQ741 886 GBEST18:AA567307 GB-EST27:Al402394 GBGSS1O:AQ237646 GBEST32:A1726448 GBEST32:A1726198 GB-PR4:AC002992 Table 4 (continued) 63734 AF127374 Streptomyces Iavendulae LinA homolog, cytochrome P450 hydroxylase ORF4, cytochrome P450 hydroxylase ORF3, WIT (mitT), MitS (mitS), MitR (mitR), MitO (mitQ), MitP (mitP), MitO (matO), MitN (mitN), MitM (mitM), Mitt. (mitL), MitK (mitK), MitJ (mitJ), Mitt (mitl), MitH (mitH), MitG (mitG), MitF (mitf), MiE (mitE), KID (mitD), MitC (mitC), MitB (mitB), MitA (mitA), MmcA (mmcA), MmcB (mmcB), MmcC (mmcC), MmcD (mmcD), MincE (mincE), MmcF (mmcF), MmcG (mmcG), MincH (mmcH-), Mmcl (mmcl), MmcJ (mmcJ), MmcK (mmcK), MmcL (mmcL), MmcM (mmcM), MmcN (mmcN), MmcO (mmcO), Mrd (mrd), MmcP (mmcP), MmcQ (mmcQ).

MmcR (mmcR), MmcS (mmcS), MVmcT (mmcT), MmcU (mmcU), MmcV (mmcV), Mct (mct), MmcW (mmcW), MmcX (mmcX), and MmcY (mmcY) genes, complete cds; and unknown genes.

742 AQ741886 HS_75569_B2_B02_8P6 RPCI-11 Human Male BAC Library Homo sapiens genomic: clone Plate=1 145 Col=4 Row=D, genomic survey sequence.

741 AA567307 HLO1-1004.5prime HL Drosophila melanogaster head BlueScript Drosophila melanogaster cONA clone HLO1004 5prime, mRNA sequence.

630 A1402394 GH21610.5prime GH Drosophila melanogaster head pOT2 Drosophila melanogaster cDNA clone GH21610 5prime, mRNA sequence.

715 AQ237646 RPCI 11-61 19.TJB RPCI-1 1 Homo sapiens genamic clone RPCI-i 1- 6119, genomic survey sequence.

562 A1726448 BNLGHi5854 Six-day Cotton fiber Gossypium hirsutum cONA 5' similar to (U5341 8) UDP-glucose dehydrogenase (Glycine max], mRNA sequence.

608 A1726198 BNLGHi5243 Six-day Cotton fiber Gossypium hirsutum cDNA 5' similar to (U5341 8) UDP-glucose dehydrogenase [Glycine max], mRNA sequence.

154848 AC002992 Homo sapiens chromosome Y, clone 203M1 3, complete sequence.

Streptomyces Iavendulae 38,411 27-MAY-1 999 Homo sapiens 38,907 16-Jul-99 rxa02558 1098 Drosophila melanogaster 38,736 Drosophila melanogaster 41.308 rxa02565 1389 Homo sapiens Gossypium hirsutum Gossypium hirsutum Homo sapiens Homo sapiens Homo sapiens 44.340 37,003 40,925 38,039 39,036 35 .957 28-Nov-98 8-Feb-99 21-Apr-99 1 1-Jun-99 11-Jun-99 1 3-OCT-i1999 17-Jul-95 23-Nov-99 rxa02574 1131 GB-EST4:H29653 415 H29653 ymS8fOi.rl Soares infant brain 1iNIB Homo sapiens cDNA clone IMAGE:52678 5 similar to SP:OXDDBOVIN P31228 D- ASPARTATE OXIDASE;, mRNIA sequence.

GB PR3:HSDJ26i K5 131974 AL050350 Human DNA sequence from dlone 261 K5 on chromosome 6q21 22. 1. Contains the 3' part of the gene for a novel organic cation transporter (BAC ORF RG331 P03), the 000 gene for 0-aspartate oxidase (EC ESTs, STSs, GSSs and two putative CpG islands, complete sequence.

2006200802 24 Feb 2006 GBEST2:R20147 rxa02589 888 rxa02592 894 rxa02603 1119 GB-HTG1 :CEY6E2 GBHTGI:.CEY6E2 GBHTG3:ACO1 1690 GB BA1 :MSGB983CS GBGSS9AQ170723 GBGSS12:AQ349397 GB-BA1:MTV026 GB_1N2:AC005714 GB-EST1 9:AA775050 GB-BA1 :MLCL373 GBBAl :MTV044 GBBA1:MLU15i8O GBEST37:A1950576 GBEST37:AI950576 GB-EST34:AV1 49547 GBEST35:AV156221 GBEST32:AV05491 9 Table 4 (continued) 494 R20147 yg18h02.rl Soares infant brain 1iNIB Homo sapiens cDNA clone IMAGE:32866 5 similar to SP:OXODBOVIN P31228 D- ASPARTATE OXIDASE;, mRNA sequence.

186306 Z96799 Caenorliabditis elegans chromosome V clone Y6E!, SEQUENCING IN PROGRESS in unordered pieces.

186306 Z96799 Caenorhabditis elegans chromosome V coY6E2, SEQUENCING IN PROGRESS in unordered pieces.

72277 AC01 1690 Homo sapiens clone 17_1 3, LOW-PASS SEQUENCE

SAMPLING.

36788 178828 Mycobacterium Ieprae cosmid 8983 DNA sequence.

487 AQ170723 HS_-2270_8 2_-F05_-MR CIT Approved Human Genomic Sperm Library 0 Homo sapiens genomic clone Plate=2270 CoI=10 Row=L, genomic survey sequence.

791 AQ349397 RPCIi11-1 18H1 6.TJ RPCI-1 I Homo sapiens genomic clone RPCI-11 11i8H 16, genom ic survey sequence.

23740 AL022076 Mycobacterium tuberculosis H37Rv complete genome; segment 157/162.

177740 AC005714 Drosophila melanogaster, chromosome 2R, region 58D4-58E2, BAC clone BACR48M13, complete sequence.

218 AA775050 ac76elO.sl Stratagene lung (#937210) Homo sapiens cDNA clone IMAGE:868554 3 similar to gb:Y00371_rnal HEAT SHOCK COGNATE 71 KO PROTEIN (HUMAN);, mRNA sequence.

37304 AL035500 Mycobacterium leprae cosniid L373.

16150 AL021999 Mycobacterium tuberculosis H37Rv complete genome; segment 45/1 62.

38675 U15180 Mycobacterium leprae cosmid 81756.

308 A1950576 wx52e08.xl NCIO GAP_-Lu28 Homo sapiens cDNA clone IMAGE:2547302 mRNA sequence.

308 A1950576 wx52e08.xl NCI_-CGAP -Lu28 Homo sapiens cDNA clone IMAGE:2547302 mRNA sequence.

302 AV149547 AV149547 Mus musculus C57BL/6J 10-1 1 day embryo Mus musculus cDNA clone 2810489003, mRNA sequence.

271 AV1 56221 AV1 56221 Mus musculus head C57BLI6J 12-day embryo Mus musculus cDNA clone 3000001 C24, mRNA sequence.

274 AV054919 AV054919 Mus musculus pancreas C57BLI6J adult Mus musculus cDNA clone 1810O033C08, mRNA sequence.

32806 AL021 184 Mycobacterium tuberculosis H37Rv complete genome; segment 64/162.

Homo sapiens Caenorhabditis elegans Caenorhabditis elegans Homo sapiens Mycobacterium leprae Homo sapiens Homo sapiens Mycobacterium tuberculosis Drosophila melanogaster Homo sapiens Mycobacterium Ieprae Mycobacterium tuberculosis Mycobacterium leprae Homo sapiens Homo sapiens 36,437 37,979 37,979 35,814 53,235 39,666 34,204 37,975 41,226 40,826 49.015 49,192 45,621 40,909 40,288 38,627 33,990 36,585 17-Apr-95 02-OCT-i 997 02-OCT-i 997 10-OCT-i1999 15-Jun-96 16-OCT-i1998 07-MAY-i 999 24-Jun-99 01-MAY-1999 5-Feb-98 27-Aug-99 17-Jun-98 09-MAR-i1995 6-Sep-99 6-Sep-99 rxaO2630 1446 rxa02643 1167 rxa02644 774 Mus musculus Mus musculus Mus musculus 5-Jul-99 7-Jul-99 23-Jun-99 rxa02745 902 GB.BAI :MTVOO7 Mycobacterium 39,298 17-Jun-98 tuberculosis 2006200802 24 Feb 2006 Table 4 (continued) GBBA2:AF027770 30683 AF027770 Mycobacterium smegmatis FxbA (fxbA) gene, partial cds; FxbB Mycobacteriumn smegmatis 55.125 (fxbB), FxbC (WxC), and FxuD (fxtD) genes, complete cds: and unknown genes.

GBBA2:SAU43537 3938 U43537 Streptomyces argillaceus mithramycin resistance determinant, ATP- Streptomyces argillaceus 46,868 binding protein (mntrA) and membrane protein (mtrB) genes, complete cds.

GBBA1:CAJ1O3l9 5368 AJ010319 Corynebactenium glutamicum amtP, glnB, ginD genes and partial ftsY Corynebacteriumn 100,000 03-DEC-i1998 5-Sep-96 rxa02746 290 I 14-LIAY-1999 GB-BA1:MTCY338 29372 Z74697 GB-HTG3AC008733 216140 AC008733 rxa02820 1411 GBBA1:BF1J64514 GB_-IN1:CETO4C1O GB-EST35:A[823090 GBBA1:CJV1 3333 GBBA2:AF065404 GB-PL2:AC006601 3837 U64514 20958 Z69885 720 A1823090 and srp genes.

Mycobacterium tuberculosis H37Rv complete genome; segment 1271162.

Homo sapiens chromosome 19 clone CITB-E1_-2525J 15, SEQUENCING IN PROGRESS 72 unordered pieces.

Bacillus firrnus dppABC operon, dipeptidle transporter protein dppA gene, partial cds, and dipeptide transporter proteins dppB and dppC genes, complete cds.

Caenorhabditis elegans cosmid TO4C1O, complete sequence.

L30-944T3 Ice plant Lambda Uni-Zap XR expression library, 30 hours NaCI treatment Mesembryanthemumn crystallinum cDNA dlone L30-944 5' similar to 60S ribosomal protein L36 (AC004684)[Arabidopsis thaliana], mRNA sequence.

Campylobacter jejuni clpB gene.

Bacillus anthracis virulence plasmid PXO1, complete sequence.

Arabidopsis thaliana chromosome V map near 60.5 cM, complete sequence.

glutamicum Mycobacterium tuberculosis Homo sapiens Bacillus firmus Caenorhabditis elegans Mesembryanthemumn crystallinumn Campylobacter jejuni Bacillus anthracis Arabidopsis thaliana 39,785 35,688 36.859 35,934 35,770 53,400 45. 168 36,680 17-Jun-98 3-Aug-99 1-Feb-97 2-Sep-99 21-Jul-99 1 2-Apr-99 20-OCT-i1999 22-Feb-99 rxa02834 518 3315 181654 110684 Y13333 AF065404 AC006601 -110- Exemplification Example 1: Preparation of total genomic DNA of Corynebacterium glutamicum ATCC 13032 A culture of Corynebacterium glutamicum (ATCC 13032) was grown overnight 0 at 30 0 C with vigorous shaking in BHI medium (Difco). The cells were harvested by 00 Scentrifugation, the supernatant was discarded and the cells were resuspended in 5 ml CN buffer-I of the original volume of the culture all indicated volumes have been

ID

Scalculated for 100 ml of culture volume). Composition of buffer-I: 140.34 g/l sucrose, N 10 2.46 g/l MgSO, x 7H 2 O, 10 ml/1 KH 2

PO

4 solution (100 g/l, adjusted to pH 6.7 with KOH), 50 ml/l M12 concentrate (10 g/l 1 g/l NaCI, 2 g/l MgSO, x 7HO, 0.2 g/l CaCl 2 0.5 g/l yeast extract (Difco), 10 ml/l trace-elements-mix (200 mg/1 FeSO, x H 2 0, 10 mg/I ZnSO, x 7 H,O, 3 mg/l MnCI, x 4 H 2 0, 30 mg/l H,BO, 20 mg/i CoCI, x 6 HO 2 1 mg/l NiCL, x 6 HO 2 3 mg/1 NaMoO 4 x 2 HO 2 500 mg/1 complexing agent (EDTA or critic acid), 100 ml/1 vitamins-mix (0.2 mg/l biotin, 0.2 mg/l folic acid, mg/1 p-amino benzoic acid, 20 mg/l riboflavin, 40 mg/l ca-panthothenate, 140 mg/l nicotinic acid, 40 mg/I pyridoxole hydrochloride, 200 mg/l myo-inositol). Lysozyme was added to the suspension to a final concentration of 2.5 mg/ml. After an approximately 4 h incubation at 37C, the cell wall was degraded and the resulting protoplasts are harvested by centrifugation. The pellet was washed once with 5 ml buffer-I and once with 5 ml TE-buffer (10 mM Tris-HCl, I mM EDTA, pH The pellet was resuspended in 4 ml TE-buffer and 0.5 ml SDS solution and 0.5 ml NaCI solution (5 M) are added. After adding ofproteinase K to a final concentration of 200 jg/ml, the suspension is incubated for ca.18 h at 37°C. The DNA was purified by extraction with phenol, phenol-chloroform-isoamylalcohol and chloroformisoamylalcohol using standard procedures. Then, the DNA was precipitated by adding 1/50 volume of 3 M sodium acetate and 2 volumes of ethanol, followed by a 30 min incubation at -20 0 C and a 30 min centrifugation at 12,000 rpm in a high speed centrifuge using a SS34 rotor (Sorvall). The DNA was dissolved in 1 ml TE-buffer containing jtg/ml RNaseA and dialysed at 4 0 C against 1000 ml TE-buffer for at least 3 hours.

During this time, the buffer was exchanged 3 times. To aliquots of 0.4 ml of the dialysed DNA solution, 0.4 ml of 2 M LiCI and 0.8 ml of ethanol are added. After a 111 min incubation at -20°C, the DNA was collected by centrifugation (13,000 rpm, SBiofuge Fresco, Heraeus, Hanau, Germany). The DNA pellet was dissolved in TE-buffer. DNA prepared by this procedure could be used for all purposes, Sincluding southern blotting or construction or genomic libraries.

N 5 EXAMPLE 2: Construction of genomic libraries in Escherichia coli of Corynebacterium glutamicum ATCC13032 0 Using DNA prepared as described in Example 1, cosmid and plasmid oo 0 libraries were constructed according to known and well established methods (see 0 cN Sambrook, J. et al. (1989) "Molecular Cloning A Laboratory Manual", Cold O 10 Spring Harbor Laboratory Press, or Ausubel, F.M. et al. (1994) "Current Protocols cl in Molecular Biology", John Wiley Sons).

Any plasmid or cosmid could be used. Of particular use were the plasmids pBR322 (Sutcliffe, J.G. (1979) Proc. Natl. Acad. Sci. USA, 75:3737-3741); pACYC177 (Change Cohen (1978) J. Bacteriol 134:1141-1156), plasmids of the pBS series (pBSSK+, pBSSK- and others; Stratagene, LaJolla, USA), or cosmids as SuperCosl (Stratagene, LaJolla, USA) or Lorist6 (Gibson, T.J., Rosenthal A. and Waterson, R.H. (1987) Gene 53:283-286. Gene libraries specifically for use in C. glutamicum may be constructed using plasmid pSL109 (Lee, and A.J. Sinskey (1994) J. Microbiol. Biotechnol. 4:256-263).

EXAMPLE 3: DNA Sequencing and Computational Functional Analysis Genomic libraries as described in Example 2 were used for DNA sequencing according to standard methods, in particular by the chain termination method using AB1377 sequencing machines (see Fleischman, R.D. et al.

(1995) "Whole-genome Random Sequencing and Assembly of Haemophilus Influenzae Rd., Science, 269:496-512). Sequencing primers with the following nucleotide sequences were used: 5'-GGAAACAGTATGACCATG-3' (SEQ ID NO.

441) or 5'-GTAAAACGACGGCCAGT-3' (SEQ ID NO. 442).

EXAMPLE 4: In vivo Mutagenesis In vivo mutagenesis of Corynebacterium glutamicum can be performed by passage of plasmid (or other vector) DNA through E. coli or other microorganisms Bacillus spp. or yeasts such as Saccharomyces cerevisiae) which are impaired in their capabilities to maintain -112- CN the integrity of their genetic information. Typical mutator strains have mutations in the genes Sfor the DNA repair system mutHLS, mutD, mutT, etc.; for reference, see Rupp, W.D.

(1996) DNA repair mechanisms, in: Escherichia coli and Salmonella, p. 2277-2294, ASM: "1 Washington.) Such strains are well known to those of ordinary skill in the art. The use of such strains is illustrated, for example, in Greener, A. and Callahan, M. (1994) Strategies 7: 32-34.

00 0O 0Example 5: DNA Transfer Between Escherichia coli and Corynebacterium

O

C- glutamicum 0Several Corynebacterium and Brevibacterium species contain endogenous C 10 plasmids (as pHM 1519 or pBL 1) which replicate autonomously (for review see, e.g., Martin, J.F. et al. (1987) Biotechnology, 5:137-146). Shuttle vectors for Escherichia coli and Corynebacterium glutamicum can be readily constructed by using standard vectors for E. coli (Sambrook, J. et al. (1989), "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press or Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley Sons) to which a origin or replication for and a suitable marker from Corynebacterium glutamicum is added. Such origins of replication are preferably taken from endogenous plasmids isolated from Corynebacterium and Brevibacterium species. Of particular use as transformation markers for these species are genes for kanamycin resistance (such as those derived from the Tn5 or Tn903 transposons) or chloramphenicol (Winnacker, E.L. (1987) "From Genes to Clones Introduction to Gene Technology, VCH, Weinheim). There are numerous examples in the literature of the construction of a wide variety of shuttle vectors which replicate in both E.

coli and C. glutamicum, and which can be used for several purposes, including gene overexpression (for reference, see Yoshihama, M. et al. (1985) J Bacteriol. 162:591-597, Martin J.F. et al. (1987) Biotechnology, 5:137-146 and Eikmanns, B.J. et al. (1991) Gene, 102:93-98).

Using standard methods, it is possible to clone a gene of interest into one of the shuttle vectors described above and to introduce such a hybrid vectors into strains of Corynebacterium glutamicum. Transformation of C. glutamicum can be achieved by protoplast transformation (Kastsumata, R. et al. (1984) J. Bacteriol. 159306-311), electroporation (Liebl, E. et al. (1989) FEMS Microbiol. Letters, 53:399-303) and in cases where special vectors are used, also by conjugation (as described e.g. in Schafer, A et al.

-113r, (1990) J. Bacteriol. 172:1663-1666). It is also possible to transfer the shuttle vectors for SC. glutamicum to E. coli by preparing plasmid DNA from C. glutamicum (using standard methods well-known in the art) and transforming it into E. coli. This transformation step can be performed using standard methods, but it is advantageous to use an Mcr-deficient E. coli strain, such as NM522 (Gough Murray (1983) J. Mol. Biol. 166:1-19).

O Genes may be overexpressed in C. glutamicum strains using plasmids which 00 Scomprise pCG1 Patent No. 4,617,267) or fragments thereof, and optionally the CN gene for kanamycin resistance from TN903 (Grindley, N.D. and Joyce, C.M. (1980) 0 Proc. Natl. Acad. Sci. USA 77(12): 7176-7180). In addition, genes may be C 10 overexpressed in C. glutamicum strains using plasmid pSL109 (Lee, and A. J.

Sinskey (1994) J. Microbiol. Biotechnol. 4: 256-263).

Aside from the use of replicative plasmids, gene overexpression can also be achieved by integration into the genome. Genomic integration in C. glutamicum or other Corynebacterium or Brevibacterium species may be accomplished by well-known methods, such as homologous recombination with genomic region(s), restriction endonuclease mediated integration (REMI) (see, DE Patent 19823834), or through the use of transposons. It is also possible to modulate the activity of a gene of interest by modifying the regulatory regions a promoter, a repressor, and/or an enhancer) by sequence modification, insertion, or deletion using site-directed methods (such as homologous recombination) or methods based on random events (such as transposon mutagenesis or REMI). Nucleic acid sequences which function as transcriptional terminators may also be inserted 3' to the coding region of one or more genes of the invention; such terminators are well-known in the art and are described, for example, in Winnacker, E.L. (1987) From Genes to Clones Introduction to Gene Technology. VCH: Weinheim.

Example 6: Assessment of the Expression of the Mutant Protein Observations of the activity of a mutated protein in a transformed host cell rely on the fact that the mutant protein is expressed in a similar fashion and in a similar quantity to that of the wild-type protein. A useful method to ascertain the level of transcription of the mutant gene (an indicator of the amount of mRNA available for translation to the gene product) is to perform a Northern blot (for reference see, for example, Ausubel et al.

-114-

C

1 (1988) Current Protocols in Molecular Biology, Wiley: New York), in which a primer Ddesigned to bind to the gene of interest is labeled with a detectable tag (usually radioactive or chemiluminescent), such that when the total RNA of a culture of the organism is extracted, run on gel, transferred to a stable matrix and incubated with this probe, the binding and quantity of binding of the probe indicates the presence and also the quantity Sof mRNA for this gene. This information is evidence of the degree of transcription of the 00 Smutant gene. Total cellular RNA can be prepared from Corynebacterium glutamicum by C several methods, all well-known in the art, such as that described in Bormann, E.R. et al.

0 (1992) Mol. Microbiol. 6: 317-326.

C 10 To assess the presence or relative quantity of protein translated from this mRNA, standard techniques, such as a Western blot, may be employed (see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: New York). In this process, total cellular proteins are extracted, separated by gel electrophoresis, transferred to a matrix such as nitrocellulose, and incubated with a probe, such as an antibody, which specifically binds to the desired protein. This probe is generally tagged with a chemiluminescent or colorimetric label which may be readily detected. The presence and quantity of label observed indicates the presence and quantity of the desired mutant protein present in the cell.

Example 7: Growth of Genetically Modified Corynebacterium glutamicum Media and Culture Conditions Genetically modified Corynebacteria are cultured in synthetic or natural growth media. A number of different growth media for Corynebacteria are both well-known and readily available (Lieb et al. (1989) Appl. Microbiol. Biotechnol., 32:205-210; von der Osten et al. (1998) Biotechnology Letters, 11:11-16; Patent DE 4,120,867; Liebl (1992) "The Genus Corynebacterium, in: The Procaryotes, Volume II, Balows, A. et al., eds.

Springer-Verlag). These media consist of one or more carbon sources, nitrogen sources, inorganic salts, vitamins and trace elements. Preferred carbon sources are sugars, such as mono-, di-, or polysaccharides. For example, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch or cellulose serve as very good carbon sources. It is also possible to supply sugar to the media via complex compounds such as molasses or other by-products from sugar refinement. It can also be 115 (N advantageous to supply mixtures of different carbon sources. Other possible carbon Ssources are alcohols and organic acids, such as methanol, ethanol, acetic acid or lactic acid. Nitrogen sources are usually organic or inorganic nitrogen compounds, or materials C which contain these compounds. Exemplary nitrogen sources include ammonia gas or ammonia salts, such as NI-IHCI or (NH,) 2 SO,, NH,OH, nitrates, urea, amino acids or complex nitrogen sources like corn steep liquor, soy bean flour, soy bean protein, yeast 00 extract, meat extract and others.

Inorganic salt compounds which may be included in the media include the chloride-, phosphorous- or sulfate- salts of calcium, magnesium, sodium, cobalt, molybdenum, potassium, manganese, zinc, copper and iron. Chelating compounds can be added to the medium to keep the metal ions in solution. Particularly useful chelating compounds include dihydroxyphenols, like catechol or protocatechuate, or organic acids, such as citric acid. It is typical for the media to also contain other growth factors, such as vitamins or growth promoters, examples of which include biotin, riboflavin, thiamin, folic acid, nicotinic acid, pantothenate and pyridoxin. Growth factors and salts frequently originate from complex media components such as yeast extract, molasses, corn steep liquor and others. The exact composition of the media compounds depends strongly on the immediate experiment and is individually decided for each specific case. Information about media optimization is available in the textbook "Applied Microbiol. Physiology, A Practical Approach (eds. P.M. Rhodes, P.F. Stanbury, IRL Press (1997) pp. 53-73, ISBN 0 19 963577 It is also possible to select growth media from commercial suppliers, like standard 1 (Merck) or BHI (grain heart infusion, DIFCO) or others.

All medium components are sterilized, either by heat (20 minutes at 1.5 bar and 121 C) or by sterile filtration. The components can either be sterilized together or, if necessary, separately. All media components can be present at the beginning of growth, or they can optionally be added continuously or batchwise.

Culture conditions are defined separately for each experiment. The temperature should be in a range between 15C and 45°C. The temperature can be kept constant or can be altered during the experiment. The pH of the medium should be in the range of 5 to 8.5, preferably around 7.0, and can be maintained by the addition of buffers to the media.

An exemplary buffer for this purpose is a potassium phosphate buffer. Synthetic buffers such as MOPS, HEPES, ACES and others can alternatively or simultaneously be used. It -116- CN is also possible to maintain a constant culture pH through the addition of NaOH or SNH.OH during growth. If complex medium components such as yeast extract are utilized, the necessity for additional buffers may be reduced, due to the fact that many complex C1 compounds have high buffer capacities. If a fermentor is utilized for culturing the microorganisms, the pH can also be controlled using gaseous ammonia.

The incubation time is usually in a range from several hours to several days. This 00 Stime is selected in order to permit the maximal amount of product to accumulate in the C broth. The disclosed growth experiments can be carried out in a variety of vessels, such as Smicrotiter plates, glass tubes, glass flasks or glass or metal fermentors of different sizes.

C 10 For screening a large number of clones, the microorganisms should be cultured in microtiter plates, glass tubes or shake flasks, either with or without baffles. Preferably 100 ml shake flasks are used, filled with 10% (by volume) of the required growth medium. The flasks should be shaken on a rotary shaker (amplitude 25 mm) using a speed-range of 100 300 rpm. Evaporation losses can be diminished by the maintenance of a humid atmosphere; alternatively, a mathematical correction for evaporation losses should be performed.

If genetically modified clones are tested, an unmodified control clone or a control clone containing the basic plasmid without any insert should also be tested. The medium is inoculated to an OD 600 of 0.5 1.5 using cells grown on agar plates, such as CM plates (10 g/1 glucose, 2,5 g/l NaCI, 2 g/l urea, 10 g/l polypeptone, 5 g/1 yeast extract, 5 g/1 meat extract, 22 g/l NaCI, 2 g/1 urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/1 agar, pH 6.8 with 2M NaOH) that had been incubated at 30"C. Inoculation of the media is accomplished by either introduction of a saline suspension of C. glutamicum cells from CM plates or addition of a liquid preculture of this bacterium.

Example 8 -In vitro Analysis of the Function of Mutant Proteins The determination of activities and kinetic parameters of enzymes is well established in the art. Experiments to determine the activity of any given altered enzyme must be tailored to the specific activity of the wild-type enzyme, which is well within the ability of one of ordinary skill in the art. Overviews about enzymes in general, as well as specific details concerning structure, kinetics, principles, methods, -117- (N applications and examples for the determination of many enzyme activities may be d found, for example, in the following references: Dixon, and Webb, (1979) Enzymes. Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism.

C1 Freeman: New York; Walsh, (1979) Enzymatic Reaction Mechanisms. Freeman: San Francisco; Price, Stevens, L. (1982) Fundamentals of Enzymology. Oxford Univ.

O Press: Oxford; Boyer, ed. (1983) The Enzymes, 3 r ed. Academic Press: New 00 0York; Bisswanger, (1994) Enzymkinetik, 2 nd ed. VCH: Weinheim (ISBN 0 N 3527300325); Bergmeyer, Bergmeyer, Gra31, eds. (1983-1986) Methods of SEnzymatic Analysis, 3 r d ed., vol. I-XII, Verlag Chemie: Weinheim; and Ullmann's C 10 Encyclopedia of Industrial Chemistry (1987) vol. A9, "Enzymes". VCH: Weinheim, p.

352-363.

The activity of proteins which bind to DNA can be measured by several wellestablished methods, such as DNA band-shift assays (also called gel retardation assays).

The effect of such-proteins on the expression of other molecules can be measured using reporter gene assays (such as that described in Kolmar, H. et al. (1995) EMBO J. 14: 3895-3904 and references cited therein). Reporter gene test systems are well known and established for applications in both pro- and eukaryotic cells, using enzymes such as beta-galactosidase, green fluorescent protein, and several others.

The determination of activity of membrane-transport proteins can be performed according to techniques such as those described in Gennis, R.B. (1989) "Pores, Channels and Transporters", in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p. 85-137; 199-234; and 270-322.

Example 9: Analysis of Impact of Mutant Protein on the Production of the Desired Product The effect of the genetic modification in C glutamicum on production of a desired compound (such as an amino acid) can be assessed by growing the modified microorganism under suitable conditions (such as those described above) and analyzing the medium and/or the cellular component for increased production of the desired product an amino acid). Such analysis techniques are well known to one of ordinary skill in the art, and include spectroscopy, thin layer chromatography, staining methods of various kinds, enzymatic and microbiological methods, and analytical -118- (N1 chromatography such as high performance liquid chromatography (see, for example, SUllman, Encyclopedia of Industrial Chemistry, vol. A2, p. 89-90 and p. 443-613, VCH: Weinheim (1985); Fallon, A. et al., (1987) "Applications of HPLC in Biochemistry" in: C Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17; Rehm et al.

(1993) Biotechnology, vol. 3, Chapter III: "Product recovery and purification", page 0 469-714, VCH: Weinheim; Belter, P.A. et al. (1988) Bioseparations: downstream 00 0processing for biotechnology, John Wiley and Sons; Kennedy, J.F. and Cabral, J.M.S.

C (1992) Recovery processes for biological materials, John Wiley and Sons; Shaeiwitz, \0 SJ.A. and Henry, J.D. (1988) Biochemical separations, in: Ulmann's Encyclopedia of C 10 Industrial Chemistry, vol. B3, Chapter 11, page 1-27, VCH: Weinheim; and Dechow, F.J. (1989) Separation and purification techniques in biotechnology, Noyes Publications.) In addition to the measurement of the final product of fermentation, it is also possible to analyze other components of the metabolic pathways utilized for the production of the desired compound, such as intermediates and side-products, to determine the overall efficiency of production of the compound. Analysis methods include measurements of nutrient levels in the medium sugars, hydrocarbons, nitrogen sources, phosphate, and other ions), measurements of biomass composition and growth, analysis of the production of common metabolites of biosynthetic pathways, and measurement of gasses produced during fermentation. Standard methods for these measurements are outlined in Applied Microbial Physiology, A Practical Approach, P.M. Rhodes and P.F. Stanbury, eds., IRL Press, p. 103-129; 131-163; and 165-192 (ISBN: 0199635773) and references cited therein.

Example 10: Purification of the Desired Product from C. glutamicum Culture Recovery of the desired product from the C. glutamicum cells or supernatant of the above-described culture can be performed by various methods well known in the art.

If the desired product is not secreted from the cells, the cells can be harvested from the culture by low-speed centrifugation, the cells can be lysed by standard techniques, such as mechanical force or sonication. The cellular debris is removed by centrifugation, and the supernatant fraction containing the soluble proteins is retained for further purification of the desired compound. If the product is secreted from the C. glutamicum -119cells, then the cells are removed from the culture by low-speed centrifugation, and the Ssupemate fraction is retained for further purification.

The supernatant fraction from either purification method is subjected to CK, chromatography with a suitable resin, in which the desired molecule is either retained on a chromatography resin while many of the impurities in the sample are not, or where the O impurities are retained by the resin while the sample is not. Such chromatography steps 00 Smay be repeated as necessary, using the same or different chromatography resins. One NC of ordinary skill in the art would be well-versed in the selection of appropriate Schromatography resins and in their most efficacious application for a particular molecule CI 10 to be purified. The purified product may be concentrated by filtration or ultrafiltration, and stored at a temperature at which the stability of the product is maximized.

There are a wide array of purification methods known to the art and the preceding method of purification is not meant to be limiting. Such purification techniques are described, for example, in Bailey, J.E. Ollis, D.F. Biochemical Engineering Fundamentals, McGraw-Hill: New York (1986).

The identity and purity of the isolated compounds may be assessed by techniques standard in the art. These include high-performance liquid chromatography (HPLC), spectroscopic methods, staining methods, thin layer chromatography, NIRS, enzymatic assay, or microbiologically. Such analysis methods are reviewed in: Patek et al. (1994) Appl. Environ. Microbiol. 60: 133-140; Malakhova et al. (1996) Biotekhnologiya 11: 27- 32; and Schmidt et al. (1998) Bioprocess Engineer. 19: 67-70. Ulmann's Encyclopedia of Industrial Chemistry, (1996) vol. A27, VCH: Weinheim, p. 89-90, p. 521-540, p. 540- 547, p. 559-566, 575-581 and p. 581-587; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons; Fallon, A. et al.

(1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17.

Example 11: Analysis of the Gene Sequences of the Invention The comparison of sequences and determination of percent homology between two sequences are art-known techniques, and can be accomplished using a mathematical algorithm, such as the algorithm of Karlin and Altschul (1990) Proc. Natl. Acad. Sci.

USA 87:2264-68, modified as in Karlin and Altschul (1993) Proc. Natl. Acad. Sci. USA -120- C 90:5873-77. Such an algorithm is incorporated into the NBLAST and XBLAST programs (version 2.0) of Altschul, et al. (1990) J. Mol. Biol. 215:403-10. BLAST nucleotide searches can be performed with the NBLAST program, score 100,

C

wordlength 12 to obtain nucleotide sequences homologous to HA nucleic acid molecules of the invention. BLAST protein searches can be performed with the SXBLAST program, score 50, wordlength 3 to obtain amino acid sequences 00 0 homologous to HA protein molecules of the invention. To obtain gapped alignments for 0 N1 comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., \0 S(1997) Nucleic Acids Res. 25(17):3389-3402. When utilizing BLAST and Gapped C 10 BLAST programs, one of ordinary skill in the art will know how to optimize the parameters of the program XBLAST and NBLAST) for the specific sequence being analyzed.

Another example of a mathematical algorithm utilized for the comparison of sequences is the algorithm of Meyers and Miller ((1988) Comput. Appl. Biosci. 4:11- 17). Such an algorithm is incorporated into the ALIGN program (version 2.0) which is part of the GCG sequence alignment software package. When utilizing the ALIGN program for comparing amino acid sequences, a PAM 120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used. Additional algorithms for sequence analysis are known in the art, and include ADVANCE and ADAM. described in Torelli and Robotti (1994) Comput. Appl. Biosci. 10:3-5; and FASTA, described in Pearson and Lipman (1988) P.N.A.S. 85:2444-8.

The percent homology between two amino acid sequences can also be accomplished using the GAP program in the GCG software package (available at http://www.gcg.com), using either a Blosum 62 matrix or a PAM250 matrix, and a gap weight of 12, 10, 8, 6, or 4 and a length weight of 2, 3, or 4. The percent homology between two nucleic acid sequences can be accomplished using the GAP program in the GCG software package, using standard parameters, such as a gap weight of 50 and a length weight of 3.

A comparative analysis of the gene sequences of the invention with those present in Genbank has been performed using techniques known in the art (see, Bexevanis and Ouellette, eds. (1998) Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins. John Wiley and Sons: New York). The gene sequences of the invention 121 CN were compared to genes present in Genbank in a three-step process. In a first step, a SBLASTN analysis a local alignment analysis) was performed for each of the sequences of the invention against the nucleotide sequences present in Genbank, and the top 500 hits were retained for further analysis. A subsequent FASTA search a combined local and global alignment analysis, in which limited regions of the sequences 0 are aligned) was performed on these 500 hits. Each gene sequence of the invention was 00 0 subsequently globally aligned to each of the top three FASTA hits, using the GAP 0 C program in the GCG software package (using standard parameters). In order to obtain Scorrect results, the length of the sequences extracted from Genbank were adjusted to the 10 length of the query sequences by methods well-known in the art. The results of this analysis are set forth in Table 4. The resulting data is identical to that which would have been obtained had a GAP (global) analysis alone been performed on each of the genes of the invention in comparison with each of the references in Genbank, but required significantly reduced computational time as compared to such a database-wide GAP (global) analysis. Sequences of the invention for which no alignments above the cutoff values were obtained are indicated on Table 4 by the absence of alignment information.

It will further be understood by one of ordinary skill in the art that the GAP alignment homology percentages set forth in Table 4 under the heading homology (GAP)" are listed in the European numerical format, wherein a represents a decimal point. For example, a value of "40,345" in this column represents "40.345%".

Example 12: Construction and Operation of DNA Microarrays The sequences of the invention may additionally be used in the construction and application of DNA microarrays (the design, methodology, and uses of DNA arrays are well known in the art, and are described, for example, in Schena, M. et al. (1995) Science 270: 467-470; Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359-1367; DeSaizieu, A. et al. (1998) Nature Biotechnology 16: 45-48; and DeRisi, J.L. et al.

(1997) Science 278: 680-686).

DNA microarrays are solid or flexible supports consisting of nitrocellulose, nylon, glass, silicone, or other materials. Nucleic acid molecules may be attached to the surface in an ordered manner. After appropriate labeling, other nucleic acids or nucleic acid mixtures can be hybridized to the immobilized nucleic acid molecules, and the label -122r, may be used to monitor and measure the individual signal intensities of the hybridized Smolecules at defined regions. This methodology allows the simultaneous quantification of the relative or absolute amount of all or selected nucleic acids in the applied nucleic cK acid sample or mixture. DNA microarrays, therefore, permit an analysis of the expression of multiple (as many as 6800 or more) nucleic acids in parallel (see, e.g., O Schena, M. (1996) BioEssays 18(5): 427-431).

00 SThe sequences of the invention may be used to design oligonucleotide primers C which are able to amplify defined regions of one or more C. glutamicum genes by a 0nucleic acid amplification reaction such as the polymerase chain reaction. The choice and design of the 5' or 3' oligonucleotide primers or of appropriate linkers allows the covalent attachment of the resulting PCR products to the surface of a support medium described above (and also described, for example, Schena, M. et al. (1995) Science 270: 467-470).

Nucleic acid microarrays may also be constructed by in situ oligonucleotide synthesis as described by Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359- 1367. By photolithographic methods, precisely defined regions of the matrix are exposed to light. Protective groups which are photolabile are thereby activated and undergo nucleotide addition, whereas regions that are masked from light do not undergo any modification. Subsequent cycles of protection and light activation permit the synthesis of different oligonucleotides at defined positions. Small, defined regions of the genes of the invention may be synthesized on microarrays by solid phase oligonucleotide synthesis.

The nucleic acid molecules of the invention present in a sample or mixture of nucleotides may be hybridized to the microarrays. These nucleic acid molecules can be labeled according to standard methods. In brief, nucleic acid molecules mRNA molecules or DNA molecules) are labeled by the incorporation of isotopically or fluorescently labeled nucleotides, during reverse transcription or DNA synthesis.

Hybridization of labeled nucleic acids to microarrays is described in Schena, M. et al. (1995) supra; Wodicka, L. et al. (1997), supra; and DeSaizieu A. et al. (1998), supra). The detection and quantification of the hybridized molecule are tailored to the specific incorporated label. Radioactive labels can be detected, for example, as 123- N described in Schena, M. et al. (1995) supra) and fluorescent labels may be detected, for example, by the method of Shalon et al. (1996) Genome Research 6: 639-645).

The application of the sequences of the invention to DNA microarray ,1 technology, as described above, permits comparative analyses of different strains of C.

glutamicum or other Corynebacteria. For example, studies of inter-strain variations 0 based on individual transcript profiles and the identification of genes that are important 00 0 for specific and/or desired strain properties such as pathogenicity, productivity and 0 C stress tolerance are facilitated by nucleic acid array methodologies. Also, comparisons \0 Sof the profile of expression of genes of the invention during the course of a fermentation 10 reaction are possible using nucleic acid array technology.

Example 13: Analysis of the Dynamics of Cellular Protein Populations (Proteomics) The genes, compositions, and methods of the invention may be applied to study the interactions and dynamics of populations of proteins, termed 'proteomics'. Protein populations of interest include, but are not limited to, the total protein population of C.

glutamicum in comparison with the protein populations of other organisms), those proteins which are active under specific environmental or metabolic conditions during fermentation, at high or low temperature, or at high or low pH), or those proteins which are active during specific phases of growth and development.

Protein populations can be analyzed by various well-known techniques, such as gel electrophoresis. Cellular proteins may be obtained, for example, by lysis or extraction, and may be separated from one another using a variety of electrophoretic techniques. Sodium dodecyl sulfate polyacrylamide gel electiophoresis (SDS-PAGE) separates proteins largely on the basis of their molecular weight. Isoelectric focusing polyacrylamide gel electrophoresis (IEF-PAGE) separates proteins by their isoelectric point (which reflects not only the amino acid sequence but also posttranslational modifications of the protein). Another, more preferred method of protein analysis is the consecutive combination of both IEF-PAGE and SDS-PAGE, known as 2-D-gel electrophoresis (described, for example, in Hermann et al. (1998) Electrophoresis 19: 3217-3221; Fountoulakis et al. (1998) Electrophoresis 19: 1193-1202; Langen et al.

(1997) Electrophoresis 18: 1184-1192; Antelmann et al. (1997) Electrophoresis 18: -124-

C

I 1451-1463). Other separation techniques may also be utilized for protein separation, Ssuch as capillary gel electrophoresis; such techniques are well known in the art.

Proteins separated by these methodologies can be visualized by standard

C

techniques, such as by staining or labeling. Suitable stains are known in the art, and include Coomassie Brilliant Blue, silver stain, or fluorescent dyes such as Sypro Ruby S(Molecular Probes). The inclusion of radioactively labeled amino acids or other protein precursors 35 S-methionine, 35 S-cysteine, 4 C-labelled amino acids, 1 acids, 5

NO

3 or 5

NH

4 or 3 C-labelled amino acids) in the medium of C. glulamicum Spermits the labeling of proteins from these cells prior to their separation. Similarly, p 10 fluorescent labels may be employed. These labeled proteins can be extracted, isolated and separated according to the previously described techniques.

Proteins visualized by these techniques can be further analyzed by measuring the amount of dye or label used. The amount of a given protein can be determined quantitatively using, for example, optical methods and can be compared to the amount of other proteins in the same gel or in other gels. Comparisons of proteins on gels can be made, for example, by optical comparison, by spectroscopy, by image scanning and analysis of gels, or through the use of photographic films and screens. Such techniques are well-known in the art.

To determine the identity of any given protein, direct sequencing or other standard techniques may be employed. For example, N- and/or C-terminal amino acid sequencing (such as Edman degradation) may be used, as may mass spectrometry (in particular MALDI or ESI techniques (see, Langen et al. (1997) Electrophoresis 18: 1184-1192)). The protein sequences provided herein can be used for the identification of C. glutamicum proteins by these techniques.

The information obtained by these methods can be used to compare patterns of protein presence, activity, or modification between different samples from various biological conditions different organisms, time points of fermentation, media conditions, or different biotopes, among others). Data obtained from such experiments alone, or in combination with other techniques, can be used for various applications, such as to compare the behavior of various organisms in a given metabolic) situation, to increase the productivity of strains which produce fine chemicals or to increase the efficiency of the production of fine chemicals.

125 NO EQUIVALENTS SThose of ordinary skill in the art will recognize, or will be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

(iComprises/comprising and grammatical variations thereof when used in 00 00 this specification are to be taken to specify the presence of stated features, Sintegers, steps or components or groups thereof, but do not preclude the C 10 presence or addition of one or more other features, integers, steps, components c or groups thereof.

Claims (21)

1. 2. An isolated nucleic acid molecule which encodes a polypeptide comprising an amino Sacid sequence selected from the group consisting of each of the even numbered SEQ SID NOs: 4 to 440, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.
2. 3. An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 440, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof.
3. 4. An isolated nucleic acid molecule comprising a nucleotide sequence which is at least identical to the entire nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 439, provided that the nucleic acid molecule does not consist of any of the F-designated genes set forth in Table 1, or a complement thereof. An isolated nucleic acid molecule comprising a fragment of at least 15 contiguous nucleotides of the nucleotide sequence of any of the odd numbered SEQ ID NOs: 3 to 439, provided that the nucleic acid molecule does not consist of any of the F- designated genes set forth in Table 1, or a complement thereof.
4. 6. An isolated nucleic acid molecule comprising the nucleic acid molecule of any one of claims 1-5 and a nucleotide sequence encoding a heterologous polypeptide. S 7. A vector comprising the nucleic acid molecule of any one of claims 1-6. S8. The vector of claim 7, which is an expression vector.
5. 9. A host cell transfected with the expression vector of claim 8. 0 The host cell of claim 9, wherein said cell is a microorganism. O 0 11. The host cell of claim 10, wherein said cell belongs to the genus Corynebacterium or N Brevibacterium.
6. 12. The host cell of claim 9, wherein the expression of said nucleic acid molecule results in the modulation in production of a fine chemical from said cell.
7. 13. The host cell of claim 12, wherein said fine chemical is selected from the group consisting of: organic acids, proteinogenic and nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides, and enzymes.
8. 14. A method of producing a polypeptide, the method comprising culturing the host cell of claim 9 in an appropriate culture medium to, thereby, produce the polypeptide. An isolated polypeptide comprising an amino acid sequence selected from the group consisting of each of the even numbered SEQ ID NOs: 4 to 440, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1.
9. 16. An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence selected from the group consisting of Seach of the even numbered SEQ ID NOs: 4 to 440, provided that the amino acid Ssequence is not encoded by any of the F-designated genes set forth in Table 1. C1 17. An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is at least 50% identical to the entire nucleic acid sequence of any of the odd numbered SEQ ID NOs: 3 to 439, provided that the nucleic acid 00 Smolecule does not consist of any of the F-designated nucleic acid molecules set forth in Table 1. CI 18. An isolated polypeptide comprising an amino acid sequence which is at least identical to the entire amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 440, provided that the amino acid sequence is not encoded by any of the F- designated genes set forth in Table 1.
10. 19. An isolated polypeptide comprising a fragment of a polypeptide comprising the amino acid sequence of any of the even numbered SEQ ID NOs: 4 to 440, provided that the amino acid sequence is not encoded by any of the F-designated genes set forth in Table 1, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino sequence. The isolated polypeptide of any one of claims 15-19, further comprising heterologous amino acid sequences.
11. 21. A method for producing a fine chemical, the method comprising culturing the cell of claim 9 such that the fine chemical is produced.
12. 22. The method of claim 21, wherein said method further comprises the step of recovering the fine chemical from said culture. (S 23. The method of claim 21, wherein said cell belongs to the genus Corynebacterium or Brevibacterium.
13. 24. The method of claim 21, wherein said cell is selected from the group consisting of: Corynebacterium glutamicum, Corynebacterium herculis, Corynebacterium, lilium, Corynebacterium acetoacidophilum, Corynebacterium acetoglutamicum, 0 Corynebacterium acetophilum, Corynebacterium ammoniagenes, Corynebacterium fujiokense, Corynebacterium nitrilophilus, Brevibacterium ammoniagenes, SBrevibacterium butanicum, Brevibacterium divaricatum, Brevibacteriumflavum, C, Brevibacterium healii, Brevibacterium ketoglutamicum, Brevibacterium ketosoreductum, Brevibacterium lactofermentum, Brevibacterium linens, Brevibacterium paraffinolyticum, and those strains set forth in Table 3. The method of claim 21, wherein expression of the nucleic acid molecule from said vector results in modulation of production of said fine chemical.
14. 26. The method of claim 21, wherein said fine chemical is selected from the group consisting of: organic acids, proteinogenic and nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides and enzymes.
15. 27. The method of claim 21, wherein said fine chemical is an amino acid.
16. 28. The method of claim 27, wherein said amino acid is drawn from the group consisting of: lysine, glutamate, glutamine, alanine, aspartate, glycine, serine, threonine, methionine, cysteine, valine, leucine, isoleucine, arginine, proline, histidine, tyrosine, phenylalanine, and tryptophan.
17. 29. A method for producing a fine chemical, the method comprising culturing a cell whose genomic DNA has been altered by the introduction of a nucleic acid molecule of any one of claims 1-6. N 30. A method for diagnosing the presence or activity of Corynebacterium diphtheriae in a Ssubject, the method comprising detecting the presence of at least one of the nucleic Sacid molecules of any one of claims 1-5 or the polypeptide molecules of any one of C"1 claims 15-19, thereby diagnosing the presence or activity of Corynebacterium diphtheriae in the subject. 00 31. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 439, wherein the nucleic acid molecule is disrupted.
18. 32. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 439, wherein the nucleic acid molecule comprises one or more nucleic acid modifications as compared to the sequence set forth in any of the odd-numbered SEQ ID NOs: 3 to 439.
19. 33. A host cell comprising a nucleic acid molecule selected from the group consisting of each of the odd numbered SEQ ID NOs: 3 to 439, wherein the regulatory region of the nucleic acid molecule is modified relative to the wild-type regulatory region of the molecule.
20. 34. A polypeptide produced by the method of claim 14. A fine chemical produced by a method of any one of claims 21 to 29. DATED this 2 4 th day of February 2006 BASF AKTIENGESELLSCHAFT WATERMARK PATENT TRADE MARK ATTORNEYS 290 BURWOOD ROAD HAWTHORN VICTORIA 3122 AUSTRALIA P20681AU01 Sseql i st cor r ect ed. t xt SEQUENCE LI STI NG <110> BASF Akt i engesel I schaf t f <120> CORYNEBACTERI UM GLUTAM CUM GENES ENCODI NG PROTEI NS (L)I NVOLVED I N HCOVECTASI S AND ADAPTATI CN <130> BGI -128CPPC <140> PCT/I B00/00911 <141> 2000-06-23 C <150> US60/141,031 0 <151> 1999-06-25 00 S <150> DE 19931636.8 0 <151> 1999-07-08 (N \C <150> DE 19932125.6 0 <151> 1999-07-09 rC <150> DE 19932126.4 <151> 1999-07-09 <150> DE 19932127.2 <151> 1999-07-09 <150> DE 19932128.0 <151> 1999-07-09 <150> DE 19932129.9 <151> 1999-07-09 <150> DE 19932226.0 <151> 1999-07-09 <150> DE 19932920.6 <151> 1999-07-14 <150> DE 19932922.2 <151> 1999-07-14 <150> DE 19932924.9 <151> 1999-07-14 <150> DE 19932928. 1 <151> 1999-07-14 <150> DE 19932930.3 <151> 1999-07-14 <150> DE 19932933.8 <151> 1999-07-14 <150> DE 19932935.4 <151> 1999-07-14 <150> DE 19932973.7 <151> 1999-07-14 <150> DE 19933002.6 <151> 1999-07-14 <150> DE 19933003.4 <151> 1999-07-14 <150> DE 19933005.0 <151> 1999-07-14 Page 1 seqI i st car r ect ed. t xt <150> DE 19933006.9 <1 51> 1999- 07- 14 <1 50> DE 19941378. 9 <1 51> 1999- 08- 31 <1 50> <151 <1 50> <1 51 <1 50> <151 <1 50> <1 51 DE 19941379.7
21. 1999- 08-31 DE 19941390.8 1999- 08-31 DE 19941391.6 1999- 08-31 DE 19942088.2 1 999- 09- 03 <1 60> 442 <210O> 1 <21 1> 314 <212> DNA <213> Corynebact er i urn gi ut anm cumn <220> <221 <222> <223> OIDS (291) RXA02 548 <400> 1 cca ccg at c t ac Pro Pro IlieTyr tcc cac gac cgc Ser His Asp Arg g aa G31u gtt ttc gag cgc Val Phe G31u Ar g gac ggc As p (1y at g t gg ct g I'bt Tr p Leu at c gt c acc I Ie Val Thr gca ggc gag tgg Al a 31y G31u Tr p ggt G31y gga cca aag aag G31y Pro Lys Lys ggc gag gag (31y (1u (1u at g t cc t gc I'bt Ser Cys aag act gt c cgc Lys Thr Val Ar g cgc acc gt c ggc Arg Thr Val Gy g at As p acc ggt Th r G3 y gct gt g ct c t cc Al a Val Leu Ser g aa G31u gcc cgc acc att Ala Arg Thr Ilie gat gt g at c gaa Asp Val Ilie Gu gag G31u at c gcc acc t cc Il e Al aThr Ser ct t acc gaa cgt Leu Thr G31u Ar g ggc G31y gca acc cgc gcc Ala Thr Arg Ala gac cgc ct C agc Asp Arg Leu Ser g aa G31u t cc gca at g gaa Ser Al a I'bt G3 u cgc aag aag gaa Ar g Lys Lys G31u ggc t ac a3 y Ty r t gat gact gc t ccaacct tg aat <210O> 2 <21 1> 97 <21 2> PRT <21 3> Cor ynebact er i urn gI ut anm cumn <400> 2 Pro Pro IlieTyr Phe Ser His Asp Arg Gu Val Phe Gu Arg Asp Gy Page 2 INO seqI i st car r ect ed. t xt 0 1 5 10 I~bt Tr pLeu Thr Aa Oy uTrp3y Oy Pro Lys Lys OyOu Ou 25 I) lIe Val Thr Lys Thr Val Arg Tyr Arg Thr Val G31y Asp I~t Ser Cys 40 Thr G31y Ala Val Leu Ser G31u Ala Ar g Thr i~e Asp Asp Va Ilie Ou 55 O31u I Ie Ala Thr Ser Thr Leu Thr O31u Ar g G31y Ala Th r Ar g Ala Asp C1 65 70 75 00 As pAr gLeu Ser 1u Ser Aia b t 1u As pAr gLys Lys (1u 1y Tyr 90 ri Phe ri <210O> 3 <21 1> 980 <212> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> <221 CIDS <222> (957) <223> RXN00249 <400> 3 acc ggc gt ctcc acc tcc cag gt t gt agt t t t gct t gt cgac gcc cgc 48 Thr (3 y Val Ser Thr Ser (3 n ValI Val Val Leu Leu Val Asp A a Ar g 1 5 10 cac ggc gtc gtc gag cag acc cgc cgc cac ctg tcc gta tcg gct ctg 96 His G31y Val Val (31u O31n Thr Ar g Ar g Hi s Leu Ser Val Ser Ala Leu 25 ct g ggc gt a cgc acg gt g at c ct c gca gt c aac aaa at t gac ct t gt t 144 Leu G31y Val Ar g Thr Val Ilie Leu Ala Val Asn Lys Ilie Asp Leu Val 40 gat t ac agc gaa gaa gt c t tc cgc aac at t gaa aag gaa t tc gt t ggc 192 Asp Tyr Ser (1u (1u Val Phe Ar gAsnIle 1u Lys(1u Phe Val (1y 55 ct g gca t ct gca ct t gat gt c aca gac acc cac gt t gt t cca at c t ct 240 Leu Ala Ser Ala Leu Asp Val Thr Asp Thr Hi s Val Val Pr o Ilie Ser 70 75 gcg ctc aag ggc gac aac gtt gca gaa cct tcc acc cac atg gat tgg 288 Ala Leu Lys G31y Asp Asn Val Ala G31u Pr o Ser Thr Hi s I'b t Asp Tr p 90 t ac acc gga cca acc gt g ct g gaa at c ct g gaa aac gt a gaa gt t t cc 336 Tyr Thr G31y Pr o Thr Val Leu O31u Ilie Leu O31u Asn Val O31u Val Ser 100 105 110 cac ggc cgt gca cac gac ctg ggc ttc cgc ttc cca atc cag tac gtc 384 His G31y Ar g Ala Hi s Asp Leu G31y Phe Ar g Phe Pro Ilie O31n Tyr Val 115 120 125 atc cgc gag cac gcc acc gac tac cgt ggc tac gcc ggc acc atc aac 432 Ilie Ar g G31u Hi s Ala Thr Asp Tyr Ar g G31y Tyr Ala G31y Thr Ilie Asn 130 135 140 Page 3 seql i st cor r ect ed. t xt gct ggt tcc gtc tcc gtg ggc gat acc gtg Ala y Ser Val Ser Val y Asp Thr Val 145 150 acc Thr gca Al a ct c Leu cgc Ar g cca Pr o 225 cgc Ar g aac Asn at c SIle gcc Al a gca Al a 305 cag gtc G n Val gtt gga Val G y 180 cgc ggc Arg G y 195 ttc aac Phe Asn gca gca Al a Al a gca gcc Al a Al a gca cca Ala Pro 260 gtt gca Val Ala 275 gga t cc G y Ser ggc ttg G y Leu acc Thr 165 gaa G u gaa G u gcc Al a gt c Val at c l Ie 245 gaa G u ggc Gy ttc Phe gtt Val cac Hi s gcc Al a ct c Leu act Thr aag Lys 230 gaa Gu acc Thr gaa G u ct c Leu ggc Gy 310 at c SIle gtt Val at c SIle gtt Val 215 gtt Val cga Ar g t ac Tyr ct c Leu ct c Leu 295 cac Hi s gat Asp gt c Val gct Al a 200 gtt Val cgc Ar g gt c Val ggc Gy gaa G u 280 at c SIle cgc Ar g t cc Ser ct g Leu 185 ggc Gy ggc Gy t ac Tyr ct c Leu ct c Leu 265 gtt Val gac Asp ct a Leu gct Al a 170 cgc Ar g gaa G u ttg Leu ggc Gy gac Asp 250 aac Asn gaa G u caa G n cgc Ar g tac cta cct gaa ggc cgc Tyr Leu Pro G u Gy Arg 155 160 gac gga tcc ctc cag acc Asp G y Ser Leu G n Thr 175 cta gcc cag gaa atc gac Leu Ala n G u Ile Asp 190 gac cgc cca gaa tcc gtt Asp Arg Pro u Ser Val 205 gcc gat cgc acc atc aaa Ala Asp Arg Thr lie Lys 220 acc gag ctg gtc cgc gga Thr G u Leu Val Arg y 235 240 atc gac ggc gtc aac gac I e Asp G y Val Asn Asp 255 gac atc gca cac gtg cgc Asp Ile Ala His Val Arg 270 gat tac gct gcc cgc ggc Asp Tyr Ala Ala Arg y 285 tcc tcc ggc gat acc ctc Ser Ser y Asp Thr Leu 300 aat aac tgg tcg atc Asn Asn Trp Ser Ile 315 tagaccagtg tcttaggcaa gac <210> 4 <211> 319 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 4 Thr G y Val Ser Thr Ser G n Val Val Val Leu Leu Val Asp Ala Arg 1 5 10 His G y Val Val G u G n Thr Arg Arg His Leu Ser Val Ser Ala Leu 25 Leu y Val Arg Thr Val lie Leu Ala Val Asn Lys Ile Asp Leu Val 40 Asp Tyr Ser G u G u Val Phe Arg Asn Ile G u Lys G u Phe Val y 55 Leu Ala Ser Ala Leu Asp Val Thr Asp Thr His Val Val Pro Ile Ser 70 75 Page 4 seqI i st car r ect ed. t xt As n VaI Ala O31u Pr o Se r Thr Hi A a Leu Lys 03 y Tyr Hi s I Ie A a 145 Thr A a Le u Ar g Pr o 225 Ar g As n I Ie A a A a 305 G31y Ar g 115 O31u Ser O31n ValI Ar g 1 95 Ph e A a A a A a ValI 275 G31y G31y As p Thr Hi s A a Ser Thr 1 65 O31u O31u A a ValI I Ie 245 O31u G31y Ph e ValI s I'bt Asp Tr p Le u Le u As p 135 G31y I Ie ValI I Ie ValI 215 ValI Ar g Tyr Le u Le u 295 Hi s I Ie 1 05 Ph e Ar g Thr Ser Le u 1 85 G31y G31y Tyr Le u Le u 265 ValI As p Le u O31u Ph e Tyr Tyr 1 As p Le u As p A a Thr 235 li1e As p As p Ser As n 315 ValI I Ie 125 G31y Pr o Ser O31n Pr o 205 Ar g Le u G31y A a A a 285 G31y Tr p O31u 110 O31n Thr O31u Le u O31u 1 O31u Thr ValI ValI Hi s 270 A a As p Ser ValI Tyr G31y O31n 1 li1e Ser I Ie Ar g As n 255 ValI Ar g Th r I Ie <210O> <21 1> 977 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (954) <223> FRXA00249 <400> ggt gt t ttc acct t ccaa ggt gt agt t t t gctt gtc gac gcc cgc cac O31y Val Phe Thr Phe O31n O31y ValI Val Leu Leu Val As p Ala Ar g Hi s 1 5 10 ggc gtc gtc gag cag acc cgc cgc cac ctg tcc gta tcg gct ctg ctg O31y Val Val O31u O31n Thr Arg Arg Hi s Leu Ser Val Ser Ala Leu Leu 25 ggc gt a cgc acg gt g at c ct c gca gt c aac aaa at t gac ct t gt t gat (3y Val Ar g Thr Val I Ie Leu Ala Val Asn Lys Ilie Asp Leu Val Asp 40 Page seql i st cor r ect ed. t xt t ac Tyr gca Al a ct c Leu acc Thr ggc G y cgc Ar g ggt Gy 145 acc Thr t ca Ser at c SIle t cc Ser ggt Sy 225 gt c Val gaa G u gac Asp at c l Ie gct Al a 305 gaa G u gca Al a ggc G y cca Pr o gca Al a 115 cac Hi s gt c Val gt c Val gga Gy ggc Gy 195 aac Asn gca Al a gcc Al a cca Pr o gca Al a 275 t cc Ser ttg Leu gt c Val gat Asp aac Asn gt g Val gac Asp acc Thr gt g Val cac Hi s 165 gcc Al a ct c Leu act Thr aag Lys gaa G u 245 acc Thr gaa G u ct c Leu ggc G y cgc Ar g aca Thr gca Al a gaa G u ggc G y t ac Tyr 135 gat Asp gat Asp gt c Val gct Al a gtt Val 215 cgc Ar g gt c Val ggc G y gaa G u at c SIle 295 cgc Ar g aac att gaa aag Asn Ile G u Lys acc Thr cct Pr o ct g Leu 105 cgc Ar g ggc G y gt g Val gct Al a cgc Ar g 185 gaa G u ttg Leu ggc G y gac Asp aac Asn 265 gaa G u caa G n cgc Ar g cac Hi s t cc Ser 90 gaa Gu ttc Phe t ac Tyr t ac Tyr gac Asp 170 ct a Leu gac Asp gcc Al a acc Thr at c SIle 250 gac Asp gat Asp t cc Ser aat Asn gtt Val 75 acc Thr aac Asn cca Pr o gcc Al a ct a Leu 155 gga Gy gcc Al a cgc Ar g gat Asp gag G u 235 gac Asp at c SIle t ac Tyr t cc Ser aac Asn 315 gaa Gu gtt Val cac Hi s gt a Val at c SIle ggc G y 140 cct Pr o t cc Ser cag G n cca Pr o cgc Ar g 220 ct g Leu ggc G y gca Al a gct Al a ggc G y 300 tgg Tr p ttc gtt Phe Val cca at c Pro Ile atg gat Iet Asp gaa gtt G u Val 110 cag t ac G n Tyr 125 acc at c Thr Ile gaa ggc G u Gy ctc cag Leu G n gaa at c G u Ile 190 gaa t cc Su Ser 205 acc at c Thr Ile gtc cgc Val Arg gtc aac Val Asn cac gtg His Val 270 gcc cgc Al a Ar g 285 gat acc Asp Thr tcg atc Ser lie ggc Gy t ct Ser tgg Tr p t cc Ser gt c Val aac Asn cgc Ar g acc Thr 175 gac Asp gtt Val aaa Lys gga G y gac Asp 255 cgc Ar g ggc G y ct c Leu ctg Leu gcg Al a t ac Tyr cac Hi s at c SIe gct Al a acc Thr 160 gca Al a ct c Leu cgc Ar g cca Pr o cgc Ar g 240 aac Asn at c SIe gcc Al a gca Al a Page 6 seqI i st car r ect ed. t xt t agaccagt g t ct taggcaa gac <210O> 6 <211> 318 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 6 G3 y ValI G3 y Val G3 y Val Ty r Ser A a Ser Leu Lys Thr G3 y G31y Ar g Ar g a31u 130 a3 y Ser 145 Thr a3 n Ser Val I Ie Ar g Ser Phe 210 a3 y A a 225 Val Ala a3 u A a As p Val I Ie a31y 290 Phe Thr Phe a31n a31y Val ValI Ar g a31u A a G y Pr o A a 115 Hi s ValI ValI a31y a31y 1 95 As n A a A a Pr o A a 275 Ser a31n ValI ValI As p As n ValI As p Thr ValI Hi s 1 65 A a Le u Thr Ly s a31u 245 Th r a31u Le u Ar g Le u Ar g 55 Thr A a a31u a31y Tyr 135 As p As p ValI A a ValI 215 Ar g ValI a31y a31u I Ie 295 Ar g A a 40 As n As p G u I Ie Ph e 120 Ar g Th r Ser Le u a y 200 a y Tyr Le u Le u ValI 280 As p Val Leu 10 Hi s Leu Val As n I Ie a31u Thr Hi s Pr o Ser 90 Leu a3 u 1 05 Ar g Phe a3 y Ty r Val Ty r A a As p 1 70 Arg Leu 1 85 a31uAs p Leu A a a3 y Thr As p II e 250 As n As p 265 a31uAs p a3 n Ser Le u Ser Lys5 Lys5 ValI Thr As n Pr o A a Le u 1 a31y A a Ar g As p a31u 235 As p I Ie Ty r Ser ValI ValI I Ie a31u ValI Hi s ValI I Ie a31y 140 Pr o Ser a31n Pr a Ar g 220 Le u a31y A a A a a31y 300 As p Ser As p Ph e Pr a vb t a31u a31n 125 Th r a31u Le u a31u a31u 205 Th r ValI ValI Hi s A a 285 As p A a A a Le u ValI I Ie As p ValI 110 Tyr I Ie a31y a31n I Ie 1 Ser I Ie Ar g As n ValI 270 Ar g Thr Ar g Le u ValI a31y Ser Tr p Ser ValI As n Ar g Thr 1 As p ValI a31y As p 255 Ar g a31y Le u Ala a1y Leu Val ay His Arg Leu Arg Asn Asn Trp Ser Ilie Page 7 seqI i st car r ect ed. t xt 305 310 315 <210O> 7 <21 1> 954 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (931) <223> RXA01073 <400> 7 taaccgactc cagcactaaa ctccaaaccc ttggcccgca ccaagacacc accgcgccat gtttgcctag gattaggtac ccgccaaagt ttagcgcgcc ac c Th r aag Ly s t cc Ser act Th r g aa O31u gcg A a gca A a ac c Th r tt c Ph e gca A a 1 50 gct A a at c I Ie gt t ValI ac a Thr gcg A a aac As n c ag O31n g ac As p gca A a 120 cgg Ar g gca A a aac As n aat As n tt c Ph e ggc G31y cga Ar g ac g Thr g at As p ag c Ser gt t ValI aat As n c ag O31n ac g Thr 1 70 gaa O31u cgt Ar g tt t Ph e ct c Le u g at As p 75 gag O31u gt g ValI gca A a at t I e t t g Le u 155 ggg O31y ct a Le u gt g ValI gt t ValI ac g Thr t at Tyr g ac As p ac c Thr gct A a aaa Ly s 140 ggc O31y Ph e aag Ly s c ag G n ct t Le u 45 c ag 0On gt c ValI g at As p gt c ValI gct A a 125 gct A a tt g Le u tt c Ph e gt g ValI tt c Ph e ggt G31y ct g Le u tt c Ph e gcg A a aac As n 110 tt g Le u cgc Ar g ct g Le u ac c Thr ag c Ser 15 ct c Le u at t I Ie gca A a t ac Tyr c aa O31n 95 gt t ValI g aa O31u c aa O31n gt t ValI aaa Ly s 1 75 cca Pr o gt c ValI t ca Ser gt a ValI gca A a 80 gt t ValI aaa Ly s ct t Le u cgc Ar g at t I Ie 1 60 tt c Ph e cgt Ar g at g vb t 1 gca A a g at As p ggt O31y gag O31u gt t ValI gca A a g ac As p cct Pr o at g vb t 145 ggc O31y ggt O31y aca aac act Thr Asn Thr at c I Ie t ac Tyr O31y cgc Ar g cgc Ar g tt g Le u gca A a gag O31u 130 gt t ValI act Thr g at As p gt g ValI cgg Ar g g at As p cgt Ar g ccc Pr o tt c Ph e 1 00 g ac As p ac c Thr C ag O31n C ac Hi s gca A a 1 C aa O31n gcc A a gct A a t cc Ser gcg A a t ac Tyr at c I Ie gcc A a g ac As p t ac Tyr gcg A a 1 gct A a gac ct g ct t cct t tg gca ggt t tg agc aag Asp Leu Leu Pro Leu Ala O31y Leu Ser Lys caa gga gct gcc att O n 0 y Al aAl a IlIe 1 Page 8 sec ct g gag cac ct g ggt gca cct t ca a Leu O31u Hi s Leu O31y Ala Pr o Ser S 200 205 gct gat ttg gaa gag gat cgc cca g Ala Asp Leu (3u (3u Asp Ar g Pr o A 215 220 ggt gt g t cg t at gcg gac at c gat a O31y Val Ser Tyr Ala Asp Ilie Asp 230 235 gt c agt gaa aaa gcc cag cag cgc a Val Ser O31u Lys Ala O31n O31n Ar g 1 250 cag cac aag cgc cac ct C cct gct a O31n Hi s Lys Ar g Hi s Leu Pr o Ala T 265 2 t aat ccaaca gt t tgagt gt cgc <210> 8 <211> 277 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 8 I'bt Thr Asn Thr O n Thr 0 u I Ie I Iist car r ect ed. t xt .gc acg t gg acc aag ier Thr Trp Thr Lys 210 cg t tg cca gat gag I a Leu Pr o Asp (3u 225 at t ac ct g gaa aac osn Tyr Leu 03 u Asn 240 .tt gag cac ct g t gg Ile O31u Hi s Leu Tr p 255 cc ccg cag gaa aat hr Pro O31n O31u Asn 70 gt t ValI g aa (31u aag Lys5 aag Lys5 t gg Tr p 275 cct Pr o gca A a ccc Pr o gt g ValI 260 t gg Tr p I e A a As p O31y O31u ValI A a As p Pr o vb t 145 O31y O31y O31n As p Le u O31n I Ie Le u O31u Thr 115 Le u A a As p O31y A a 1 95 A a A a Ser A a Tyr li1e A a As p Tyr A a 1 65 A a I Ie Lys5 Ser Thr O31u 70 A a A a Thr Ph e A a 150 A a As p Le u O31u Hi s Le u O31u I Ie Pr o O31u As n 135 li1e O31u Le u O31u ValI Thr 40 A a As n O31n As p A a 120 Ar g A a As n Le u Hi s 200 O31u Lys5 O31y Ser A a Lys5 1 Thr O31y O31y ValI Pr a 1 Le u O31u Ar g Ph e Le u As p 75 O31u ValI A a I Ie Le u 1 55 O31y A a A a Ly s O31n Le u O31n ValI As p ValI A a 125 A a Le u Ph e Le u Ser 205 Ser Le u I Ie A a Tyr O31n ValI O31u O31n ValI Ly s 1 Ly s Thr Pr a ValI Ser ValI A a ValI Ly s Le u Ar g I Ie 1 Ph e Ar g Tr p Page 9 seql i st cor r ect ed. t xt Thr Lys Val Pro Thr Ala Asp Leu u u Asp Arg Pro Ala Leu Pro 210 215 220 Asp G u G u Ala Leu G y Val Ser Tyr Ala Asp Ile Asp Asn Tyr Leu 225 230 235 240 Su Asn Lys Pro Asp Val Ser u Lys Ala On On Arg lie u His 245 250 255 Leu Trp Lys Val G y G n His Lys Arg His Leu Pro Ala Thr Pro G n 260 265 270 G u Asn Trp Trp Arg 275 <210> 9 <211> 609 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(586) <223> RXN02913 <400> 9 tccctgacat ccaggttgaa gcaacgtttg atgacggcac atcccatccg ataacccttg atgtttttag gagttttgtc caagctcgtc accgtgcaca t ac Tyr aaa Lys tcg Ser agt Ser cgc Ar g ggt Sy gag Su cgt Ar g gct Al a ct g Leu at c SIle ttc Phe ggt Sy gag Su cgc Ar g ct c Leu t ca Ser 120 gaa Gu agc Ser at c SIle ttc Phe ggc G y ggc G y gtt Val gt c Val gct Al a ggc G y t ca Ser aac Asn gaa G u cgc Ar g gcc Al a ggt G y gt g Val gat Asp aaa Lys 140 ct c Leu acc Thr gt a Val 45 ctt Leu cgc Ar g ttc Phe tgg Tr p gct Al a 125 ct a Leu acc Thr ggt G y aac Asn gat Asp acc Thr cga Ar g gag G u 110 cca Pr o gat Asp gga G y 15 gat Asp ccc Pr o at t SIle gt c Val gat Asp 95 gga G y caa G n aat Asn agg Ar g agc Ser cca Pr o aac Asn 80 ct c Leu cgc Ar g gaa G u at g Met 1 gtt Val cct Pr o at c SIle t cc Ser ctt Leu gt c Val gaa G u ttg Leu atc cca ggc gag lie Pro Gy u ggg cgc gag gcc G y Arg G u Ala gtg cag att ggt Val n lie G y agc ttt gat cgc Ser Phe Asp Arg ggc acc gcg gtg G y Thr Ala Val gtt gcc att ggc Val Ala lIe y gat ggg ccg ttg Asp y Pro Leu 100 gac ggt tgg cgt Asp y Trp Arg 115 cca cag gtc gaa Pro G n Val G u 130 gac gcc act gat gtg gac aca Asp Ala Thr Asp Val Asp Thr 145 Page seqI i st car r ect ed. t xt aat gt g ggc aca gaa gaa ggc t tt gaa gaa ggt cga aat t aaat gagt t As n Va 1G31y Thr G31u 31u G31y Phe G31u 31u G31y Ar g As n 150 155 160 t tgagat t tc ccg <210O> <211> 162 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> I'b t I Ie Val 31y. Pr o Val I I e Ser Ser G3 y Leu Val Val As p 31u As p Leu Pro 130 Thr As p 145 Ar g As n Pro G31y Ar g G31u (31n I Ie Phe As p Thr A a A a I I e (31y Pr o 1 00 (31y Tr p 115 (3 n Val Val As p G31u A a G31y Ar g ValI G31y Le u Ar g G31u Th r Tyr I Ie Leu Ser Ly s Ser Ser Ar g 70 G31y G31u Ar g A a As n 150 Thr Hi s G31u Le u As p As p Ser A a 135 ValI G31u Hi s Tyr Pr o I Ie Ly s 1 A a Ar g Thr Ser 10 I Ie Ph e G31y G31y ValI 90 ValI A a G31y (31u G3 u Ser Leu Thr I Ie A a Ph e As p A a As n G31y Ar g G31u 1 Thr ValI Le u Ar g Ph e Tr p A a 125 Le u Ph e G31y As p Pr o I Ie ValI As p G31y (31n As p (31u <210O> 11 <21 1> 220 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 OIDS <222> (220) <223> FRXA02264 <400> 11 t ccct gacat ccaggt t gaa gcaacgt t t g at gacggcac caagct cgt c accgt gcaca at cccat ccg at aaccct tg at gt tt t tag gagtt t tgt c at g atc cca ggc gag 115 I'b t Ilie Pro G1y Gu 1 tac atc ctg tcc agc gaa tca ctc accgga aat gtt ggg cgc gag gcc 163 Tyr I Ie Leu Ser Ser (3u Ser Leu Thr (3y Asn Val (3y Ar g G31u Ala 15 Page 11 seql i st cor r ect ed. t xt aaa acc at c Lys Thr Ile tcg cat ttc Ser Hi s Phe gaa atc atc aac acc ggt gat agg cct gt g Ou lie lie Asn Thr Gy Asp Arg Pro Val 30 cag att ggt n lie G y <210> <211> <212> <213> 12 PRT Cor ynebact eri um gl ut am cum <400> 12 NIet Ile Pro Gy Gu Tyr Ile Leu Ser Ser 1 5 10 Val y Arg Gu Ala Lys Thr lie Gu lie 25 G u Ser Leu Thr Sy Asn I e Asn Thr G y Asp Arg Pro Val n IIe Gy Ser His Phe <210> <211> <212> <213> <220> <221> <222> <223> 13 1833 DNA Cor ynebact eri um gl ut am cum CDS (101) (1810) RXN02274 <400> 13 acaggtcgaa gctgctgaac gtggccggaa actagatgac tgtgggcaca gaagaaggct ttgaagaagg tcgaaattaa gccactgatg tggacacaaa agt ttt gag Ser Phe G u tcc cgc aag cag Ser Arg Lys G n t ac Tyr acc gac ctt tat Thr Asp Leu Tyr cca acc gtt ggc Pro Thr Val G y gat tca Asp Ser gt a cgt ct t Val Arg Leu gca gca at c Al a Al a I e gat act gag ctt Asp Thr G u Leu ctc tgt gtg gaa Leu Cys Val G u aaa gat tac Lys Asp Tyr gtc att cgt Val I I e Arg ggc gaa gaa gt a G y G u G u Val gca Al a 45 ttc ggc ggt ggc Phe G y G y G y aag Lys gat ggc Asp y atg ggc caa aat IVet y G n Asn ggc Gy acc ttg gtt cgc Thr Leu Val Arg gta gat att ccc Val Asp lie Pro gat Asp acc gtc atc acc Thr Val Ile Thr gtc atc gtc ct t Val Ile Val Leu gac Asp tat acg ggt gtg Tyr Thr G y Val aaa gct gac gtt Lys Al a Asp Val gcg Al a ctt cga gat ggc Leu Arg Asp y aaa Lys 95 atc ttc cga atc Ile Phe Arg Ile gga aag G y Lys 100 gcc gga aac ccg aat gtc atg gaa aac gtc gac atc gtc atc ggc gtt Page 12 seqi i st car r ect ed. t xt Ala G1y Asn Pro Asn Val libt Ou Asn Val Asp lieVal I I e 03 y Val1 115 1 05 gcc A a at c I Ie tt a Le u 1 50 c ag O31n aac As n ggt G31y gcg A a t cg Ser 230 gt g ValI ac c Thr ggt G31y cca Pr o aac As n 310 aac As n gcc A a t ct Ser g ac As p 120 ac g Thr t ca Ser t cg Ser ct t Le u ggc G31y 200 gca A a at c I Ie ct c Le u g aa O31u ggt G31y 280 gt g ValI gt t ValI g at As p ac g Thr ac c Thr 360 at t I Ie gt g ValI at c I Ie gct A a 1 70 gct A a ggt G31y ggt G31y act Thr t cc Ser 250 at t I Ie gga G31y cct Pr o gag O31u cca Pr o 330 gca A a t cg Ser gct A a cac Hi s acc Thr 155 aca Thr tt t Ph e t ct Ser ct g Le u gcc A a 235 g at As p gcg A a cac Hi s gca A a cac Hi s 315 gaa O31u gcc A a g at As p ggt G31y tt c Ph e 140 ac g Thr act Thr g aa O31u t cc Ser aaa Ly s 220 ct a Le u ac c Thr ggc G31y gct A a t cc Ser 300 ct g Le u g ac As p g aa O31u t cc Ser g aa O31u 125 tt g Le u at g vb t gt c ValI ggc G31y aaa Ly s 205 at t I Ie g aa O31u tt g Le u cga Ar g cct Pr o 285 ac c Thr g ac As p gt g ValI g at As p c ag O31n 365 110 ggc G31y G31y at c I Ie ac g Thr at g vb t 1 90 t ct Ser c ac Hi s gt a ValI aat As n gt c ValI 270 g ac As p aac As n at g vb t gct A a gt g ValI 350 gcg A a aaa Ly s ac a Thr ggt G31y cca Pr a 1 75 ccc Pr a ccg Pr a gag O31u gcc A a gag O31u 255 at c I Ie ct a Le u cca Pr a gt g ValI tt t Ph e 335 ct t Le u at g vb t at c I Ie g ac As p gga G31y 1 60 ggt G31y at g vb t ct g Le u g ac As p g at As p 240 gcc A a cat Hi s at c I Ie ac g Thr at g vb t 320 gcg A a c ac Hi s 99c G31y ct t Le u c ag O31n 145 99c G31y c ag O31n aac As n gct A a t gg Tr p 225 g ac As p ggt O31y ac c Thr cga Ar g ct c Le u 305 gt t ValI g at As p g at As p cga Ar g ac c Thr 130 gt c ValI ac c Thr t gg Tr p tt t Ph e gag O31u 210 ggt O31y at g vb t tt t Ph e tt c Ph e gt g ValI 290 cca Pr a gcc A a t cc Ser at g vb t gt a ValI 370 gca A a aac As n ggc O31y aat As n ggc O31y 1 c ag O31n gcc A a g ac As p gt g ValI c ac Hi s 275 gct A a t ac Tyr c ac Hi s cga Ar g ggt O31y 355 gga O31y ggt O31y act Thr cca Pr a ac c Thr 1 at t I Ie gt t ValI ac a Thr at c I Ie g aa O31u 260 ac c Thr gct A a ac c Thr c ac Hi s at t I Ie 340 at c I Ie gag O31u ggc O31y gca A a ag c Ser 1 t ac Tyr tt g Le u cgt Ar g cca Pr a c ag O31n 245 g ac As p g aa O31u ct g Le u cga Ar g ct c Le u 325 cgt Ar g tt c Ph e ac c Thr 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 at c acg cgc acg t gg cag gt c gcc gac cat at g aaa cgc acc cgt gga Page 13 II e Thr Ar g 375 Thr Trp n Val Ala 380 eql i st cor r ect Asp His Met ed. txt t ca Ser 390 ttc Phe t at Tyr tgg Tr p ggg Gy act Thr 470 gca Al a gca Al a cga Ar g at g Met ggt Sy 550 cgc Ar g acg Thr gca Al a gtt Val cca Pr o 440 at g Met cag G n t cc Ser gtt Val gt a Val 520 gat Asp ttg Leu ttc Phe gat gct Asp Al a 395 tac acc Tyr Thr 410 t ca gt g Ser Val ttc ttt Phe Phe aat t cc Asn Ser cgc acc Arg Thr 475 agc t cc Ser Ser 490 gat ct g Asp Leu aat ct g Asn Leu cgt gtc Arg Val acc agc Thr Ser 555 ttc taat Phe 570 cca Pr o at c SIle gag G u ggt Gy ct c Leu 460 ct g Leu at t SIle ct g Leu acc Thr gat Asp 540 aag Lys t ac Tyr aac Asn gaa G u gt g Val 445 at g Met cgc Ar g aca Thr aat Asn aaa Lys 525 cca Pr o cca Pr o aac Asn cct Pr o ggc Gy 430 aaa Lys ggt Gy aat Asn ttc Phe ct g Leu 510 cga Ar g gag G u gca Al a gac Asp gcg Al a 415 aag Lys cct Pr o gat Asp act Thr ct a Leu 495 agg Ar g gac Asp acc Thr gag G u aac Asn 400 at t l Ie ttc Phe gat Asp t cc Ser tgg Tr p 480 t cc Ser aag Lys at g Met t ac Tyr aca Thr 560 Lys 385 aac Asn gcg Al a gct Al a ct g Leu aac Asn 465 ggt G y cag G n cag G n aaa Lys cag G n 545 gt g Val cgc Ar g cac Hi s gac Asp gt g Val 450 ggt Gy gcg Al a gac Asp at c SIle ct c Leu 530 gt g Val cca Pr o cgt Ar g gt g Val 420 gt g Val aag Lys at t SIle ggc Gy at c SIle 500 ggc G y gca Al a gt c Val gca Al a cga Ar g 405 gat Asp ct g Leu ggt Gy cca Pr o cag G n 485 gat Asp gtt Val gaa G u aac Asn cgt Ar g 565 Arg Thr Arg G y 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1833 ccgcca acaaggaagg aag <210> 14 <211> 570 <212> PRT <213> Corynebact <400> 14 bet Ser Phe G u 1 Thr Val y Asp Val G u Lys Asp Sy Lys Val lie er i um gl ut ari cum I e Ser Arg Lys G n Tyr Thr Asp Leu Tyr y Pro 5 10 Ser Val Arg Leu Ala Asp Thr G u Leu Phe Leu Cys 25 Tyr Ala Ala Ile Oy G u Ou Val Ala Phe y y 40 Arg Asp y IVet y n Asn y Thr Leu Val Arg Page 14 seqI i st car r ect ed. t xt As p Tyr Ph e I Ie Le u O31n 145 G31y O31n As n Al a Tr p 225 As p G31y Thr Ar g Le u 305 ValI As p As p Ar g Ly s 385 As p G31y I Ie I Ie 115 Al a As n G31y As n G31y 1 95 O31n Al a As p ValI Hi s 275 Al a Tyr Hi s Ar g G31y 355 G31y Thr Pr o Tyr Ly s ValI G31y Al a Ser 1 65 Tyr Le u Ar g Pr o O31n 245 As p O31u Le u Ar g Le u 325 Ar g Ph e Thr O31y Ar g 405 Asp Thr Val 70 Lys Al a As p Al a 03 y As n Al a Thr As p 120 1 1 e As p Thr 135 Leu Al a Ser 150 O3 n Al a Ser As n I'bt Leu O3 y Lys 03 y 200 Al a 03 y Al a 215 Ser Ser I Ie 230 Val Al a Leu Thr I Ie O31u O3 y Al a 03 y 280 Pr o As n Val 295 As n Thr Val 310 As n Pr o As p Al a 03 u Thr Ser I Ie Thr 360 li1e Thr Ar g 375 Ser Leu Thr 390 Phe I I e Al a 1 1 e Thr Asn Val 75 Val Ala Leu Arg 90 Pr o Asn Val I'b t 1 05 Ile IlIe Al a 03 y Hi s Val Hi s Phe 140 O3 y Il e Thr Thr 1 55 I'b t Al a Thr Thr 1 70 Ser Al a Phe 03 u 1 85 Hi s 03 y Ser Ser Il e 03 y Leu Lys 220 Asn Thr Al a Leu 235 Hi s Ser Asp Thr 250 Al a IlIe Al a 03 y 265 O3 y 03 y Hi s Al a Leu Pr o Al a Ser 300 O3 u 03 u Hi s Leu 315 Ilie Pr o O31u Asp 330 Ile Al a Al a 03 u 345 Ser Ser Asp Ser Thr Tr p O31n Val 380 O31y Asp Al a Pr o 395 I Ie Val Leu As p O31u O31u 125 Le u vb t ValI O31y Ly s 205 li1e O31u Le u Ar g Pr o 285 Thr As p ValI As p O31n 365 Al a Tyr (31y As n 110 G31y Gy le Thr vb t 1 Ser Hi s ValI As n ValI 270 As p As n vb t Al a ValI 350 Al a As p As n Ly s ValI Ly s Thr G31y Pr a 1 Pr a Pr a O31u Al a O31u 255 li1e Le u Pr a ValI Ph e 335 Le u vb t Hi s As p As p lie As p I Ie As p G31y 1 G31y vb t Le u As p As p 240 Al a Hi s I Ie Thr vb t 320 Al a Hi s G31y vb t As n 400 Asn Arg Leu Arg Ly s Thr lieAsn Pro Ala Ilie 415 Page INO seql i st cor r ect ed. t xt SAla His Gy Val Asp Tyr Val Val Arg Ser Val G u G u G y Lys Phe 420 425 430 Ala Asp Leu Val Leu Trp Asp Pro Lys Phe Phe G y Val Lys Pro Asp 435 440 445 d) I Leu Val Ile Lys y G y Leu Iet Val Asn Ser Leu IVet y Asp Ser 450 455 460 Asn y Ser lie Pro Thr Pro G n Pro Arg Thr Leu Arg Asn Thr Trp 465 470 475 480 C 1 y Ala Phe y G n Al a Val Ser Arg Ser Ser lie Thr Phe Leu Ser 485 490 495 00 0 G n Asp Ala lie Asp Al a Asn Val Pro Asp Leu Leu Asn Leu Arg Lys 500 505 510 O G n Ile Arg y Val Arg y Val Arg Asn Leu Thr Lys Arg Asp IVet 515 520 525 Cq Lys Leu Asn Ala G u Iet Pro Asp Ile Arg Val Asp Pro G u Thr Tyr 530 535 540 G n Val Phe Val Asn G y G u Leu Ile Thr Ser Lys Pro Ala G u Thr 545 550 555 560 Val Pro IVet Ala Arg Arg Tyr Phe Leu Phe 565 570 <210> <211> 1625 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (1)..(1602) <223> FRXA02274 <400> tac gca gca atc ggc gaa gaa gta gca ttc ggc ggt ggc aag gtc att 48 Tyr Al a Ala I Ie y u u Val Al a Phe G y y y Lys Val lie 1 5 10 cgt gat ggc atg ggc caa aat ggc acc ttg gtt cgc gat gta gat att 96 Arg Asp y IVet y G n Asn y Thr Leu Val Arg Asp Val Asp Ile 25 ccc gat acc gtc atc acc aac gtc atc gtc ctt gac tat acg ggt gtg 144 Pro Asp Thr Val lie Thr Asn Val lie Val Leu Asp Tyr Thr y Val 40 tac aaa gct gac gtt gcg ctt cga gat ggc aaa atc ttc cga atc gga 192 Tyr Lys Ala Asp Val Al a Leu Arg Asp y Lys Ile Phe Arg lie y 55 aag gcc gga aac ccg aat gtc atg gaa aac gtc gac atc gtc atc ggc 240 Lys Al a y Asn Pro Asn Val IVet G u Asn Val Asp Ile Val I le y 70 75 gtt gcc acc gac atc att gct ggt gaa ggc aaa atc ctt acc gca ggt 288 Val Al a Thr Asp lie ile Ala y G u y Lys Ile Leu Thr Al a y 90 ggc atc gac acg cac gtg cac ttc ttg ggc aca gac cag gtc aac act 336 G y IIe Asp Thr His Val His Phe Leu G y Thr Asp G n Val Asn Thr Page 16 seqi i st car r ect ed. t xt gca A a ag c Ser t ac Tyr 145 t tg Le u cgt Ar g cca Pr o c ag O31n g ac As p 225 g aa O31u ct g Le u cga Ar g ct c Le u cgt Ar g 305 t tc Ph e ac c Thr gga G31y gca A a 115 gcg A a at g vb t aaa Ly s ggt G31y t cg Ser 1 95 gca A a at t I Ie gct A a aac As n act Thr 275 cca Pr o g aa O31u at c I Ie ac g Thr ct a Le u 355 ggt G31y at g vb t agt Ser cat Hi s 1 65 at c I Ie aac As n c ac Hi s gcc A a ggt G31y 245 tt g Le u g aa O31u at t I Ie at t I Ie t ct Ser 325 ac g Thr gga G31y at c I Ie gct A a gct A a 150 ggt G31y ggt G31y act Thr t cc Ser at t I Ie 230 gga G31y cct Pr o gag O31u cca Pr o gca A a 310 t cg Ser t gg Tr p g at As p ac c Thr ac a Thr 135 tt t Ph e t ct Ser ct g Le u gcc A a g at As p 215 gcg A a c ac Hi s gca A a c ac Hi s g aa O31u 295 gcc A a g at As p c ag O31n gct A a ac g Thr 120 act Thr g aa O31u t cc Ser aaa Ly s ct a Le u 200 ac c Thr ggc G31y gct A a t cc Ser ct g Le u 280 g ac As p g aa O31u t cc Ser gt c ValI cca Pr a 360 1 at g vb t gt c ValI G31y aaa Ly s at t I Ie 1 85 g aa O31u tt g Le u cga Ar g cct Pr a ac c Thr 265 g ac As p gt g ValI g at As p c ag O31n gcc A a 345 t ac Tyr at c I Ie ac g Thr at g vb t t ct Ser 1 70 c ac Hi s gt a ValI aat As n gt c ValI g ac As p 250 aac As n at g vb t gct A a gt g ValI gcg A a 330 g ac As p aac As n ggt G31y cca Pr a ccc Pr a 1 55 ccg Pr a gag O31u gcc A a gag O31u at c I Ie 235 ct a Le u cca Pr a gt g ValI tt t Ph e ct t Le u 315 at g vb t cat Hi s g ac As p gcg A a gga G31y ggt G31y 140 at g vb t ct g Le u g ac As p g at As p gcc A a 220 cat Hi s at c I Ie ac g Thr at g vb t gcg A a 300 c ac Hi s 99c G31y at g vb t aac As n 99c G31y 125 c ag O31n aac As n gct A a t gg Tr p g ac As p 205 ggt O31y ac c Thr cga Ar g ct c Le u gt t ValI 285 g at As p g at As p cga Ar g aaa Ly s aac As n 365 ac c Thr t gg Tr p tt t Ph e gag O31u ggt O31y 1 at g vb t tt t Ph e tt c Ph e gt g ValI cca Pr a 270 gcc A a t cc Ser at g vb t gt a ValI cgc Ar g 350 cgc Ar g ggc O31y aat As n ggc O31y c ag O31n 1 gcc A a g ac As p gt g ValI c ac Hi s gct A a 255 t ac Tyr c ac Hi s cga Ar g ggt O31y gga O31y 335 ac c Thr tt g Le u cca Pr a ac c Thr at t I Ie 1 gt t ValI ac a Thr at c I Ie g aa O31u ac c Thr 240 gct A a ac c Thr c ac Hi s at t I Ie at c I Ie 320 gag O31u cgt Ar g cgt Ar g 384 432 480 528 576 624 672 720 768 816 864 912 960 1 008 1 056 1104 1152 cga t tc at c gca Ar g Ph e I Ie Ala aaa t ac acc at c aac cct Lys Tyr Thr lie Asn Pro at t gcg cac ggt gt g llIe Al a Hi s 0 y Val Page 17 seql i st cor r ect ed. t xt 375 gat tat gtt gtt cgt tca gtg gag gaa Asp Tyr Val Val Arg Ser Val G u G u 385 390 ctg tgg gat cca aag ttc ttt ggt gtg Leu Trp Asp Pro Lys Phe Phe y Val 405 ggt ggg ttg atg gtc aat tcc ctc atg Gy y Leu IVet Val Asn Ser Leu IVet 420 425 cca act ccg cag ccc cgc acc ctg cgc Pro Thr Pro G n Pro Arg Thr Leu Arg 435 440 cag gca gtt tcc aga agc tcc att aca G n Ala Val Ser Arg Ser Ser lie Thr 450 455 gat gca aat gtt cct gat ctg ctg aat Asp Ala Asn Val Pro Asp Leu Leu Asn 465 470 gtt cga ggt gta agg aat ctg acc aaa Val Arg y Val Arg Asn Leu Thr Lys 485 gaa atg cct gat att cgt gtc gat cca G u IVet Pro Asp lie Arg Val Asp Pro 500 505 aac ggt gag ttg atc acc agc aag cca Asn Gy G u Leu Ile Thr Ser Lys Pro 515 520 cgt cgc tac ttc ttg ttc taatccgcca Arg Arg Tyr Phe Leu Phe 530 <210> 16 <211> 534 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 16 Tyr Ala Ala Ile G y u Ou Val Ala 1 5 Arg Asp Gy IVet Gy n Asn Gy Thr Pro Asp Thr Val lie Thr Asn Val lle 40 Tyr Lys Ala Asp Val Ala Leu Arg Asp 55 Lys Al a G y Asn Pro Asn Val IVet G u 70 Val Al a Thr Asp lie lle Ala G y u G y IIe Asp Thr His Val His Phe Leu ggc Gy aaa Lys 410 ggt Gy aat Asn ttc Phe ct g Leu cga Ar g 490 gag G u gca Al a aag Lys 395 cct Pr o gat Asp act Thr ct a Leu agg Ar g 475 gac Asp acc Thr gag G u 380 ttc gct Phe Al a gat ctg Asp Leu t cc aac Ser Asn tgg ggt Trp y 445 t cc cag Ser G n 460 aag cag Lys G n atg aaa Vet Lys tac cag Tyr G n aca gt g Thr Val 525 gac Asp gt g Val ggt Gy 430 gcg Al a gac Asp at c SIle ct c Leu gt g Val 510 cca Pr o ct c Leu at c SIle 415 t cc Ser ttt Phe gct Al a cgg Ar g aat Asn 495 ttt Phe at g Met gt g Val 400 aag Lys at t SIe ggc Gy at c SIe ggc Gy 480 gca Al a gt c Val gca Al a 1200 1248 1296 1344 1392 1440 1488 1536 1584 1625 acaaggaagg aag Phe G y G y G 10 Leu Val Arg A Val Leu Asp TI G y Lys Ile P Asn Val Asp II G y Lys Ile L 90 G y Thr Asp G Page 18 y sp yr he e eu n Val Asp Gy I e I e Al a Asn seql i st cor r ect ed. t xt 105 e t lie G y G y Al a Leu Ser G n 130 Tyr Asn 145 Leu G y Arg Ala Pro Ser G n Val 210 Asp Thr 225 G u G y Leu Pro Arg Asn Leu Asn 290 Arg Ala 305 Phe Ser Thr Ile G y Ser Arg Phe 370 Asp Tyr 385 Leu Trp G y G y Pro Thr G n Al a 450 Al a 115 Al a vbt Lys Sy Ser 195 Al a SIle Al a Asn Thr 275 Pr o Su I e Thr Leu 355 I le Val Asp Leu Pr o 435 Ser G y Ser Met Leu Ser G y Hi s 165 Ala I e 180 Ile Asn Leu Hi s G u Al a G y G y 245 Val Leu 260 Val G u Asp Il e Thr Ile Thr Ser 325 Arg Thr 340 Thr G y Al a Lys Val Arg Pro Lys 405 [bt Val 420 G n Pro Il e Thr Thr Al a Al a 150 Sy Sy Thr Ser l Ie 230 Sy Pr o G u Pr o Al a 310 Ser Tr p Asp Tyr Ser 390 Phe Asn Ar g Thr 135 Phe Ser Leu Al a Asp 215 Al a Hi s Al a Hi s G u 295 Al a Asp G n Al a Thr 375 Val Phe Ser Thr Val Sy Lys SIle 185 G u Leu Ar g Pr o Thr 265 Asp Val Asp G n Al a 345 Tyr Asn G u Val Lb t 425 Ar g Thr Met Ser 170 Hi s Val Asn Val Asp 250 Asn bet Al a Val Al a 330 Asp Asn Pr o Sy Lys 410 Sy Asn Pr o Pr o 155 Pr o G u Al a Su I e 235 Leu Pr o Val Phe Leu 315 bet Hi s Asp Al a Lys 395 Pr o Asp Thr Gy 140 Met Leu Asp Asp Al a 220 Hi s SIle Thr bet Al a 300 Hi s Sy vbt Asn l Ie 380 Phe Asp Ser Tr p Sy 125 G n Asn Al a Tr p Asp 205 Sy Thr Ar g Leu Val 285 Asp Asp Ar g Lys Asn 365 Al a Al a Leu Asn Sy 445 110 Thr G y Pro Trp Asn Thr Phe G y lie 160 Su G n Val 175 G y Al a Thr 190 IVet Asp I e Phe Val G u Phe Hi s Thr 240 Val Al a Al a 255 Pro Tyr Thr 270 Al a Hi s Hi s Ser Arg Il e 'e t y lie 320 Val y u 335 Arg Thr Arg 350 Arg Leu Arg Hi s G y Val Asp Leu Val 400 Val Ile Lys 415 Sy Ser lle 430 Al a Phe G y Val Ser Arg Ser Ser Ile Thr Phe Leu Ser G n Asp Ala lie 455 460 Page 19 seql i st cor r ec Asp Ala Asn Val Pro Asp Leu Leu Asn Leu Arg 465 470 475 Val Arg y Val Arg Asn Leu Thr Lys Arg Asp 485 490 G u IVet Pro Asp Ile Arg Val Asp Pro G u Thr 500 505 Asn Gy u Leu Ile Thr Ser Lys Pro Ala u 515 520 Arg Arg Tyr Phe Leu Phe 530 <210> 17 <211> 423 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(400) <223> RXA02265 <400> 17 tcaaaggaaa gcagtatatt gtcggttttt tagaggctct agctaaacta atttctgtta cctgacagaa aggggcaaaa ted. txt Lys On lie Arg Gy 480 IVet Lys Leu Asn Al a 495 Tyr G n Val Phe Val 510 Thr Val Pro Met Ala 525 cccaaatatt gcgttggggg ttg cat atc act cct Leu His Ile Thr Pro 1 cgt gaa caa gaa aaa ctg atg atc gtg gtg gcg gct gac ctt gca cgt Arg G u n G u Lys Leu IVet lie Val Val Ala Ala Asp Leu Ala Arg 15 cgc cgt aaa gat cgc ggc cta aaa ctt aac cac cca gag gcc gtc gcc Arg Arg Lys Asp Arg G y Leu Lys Leu Asn His Pro G u Ala Val Ala 30 ctc atc acg tat gaa ctg att gaa ggc gcc cgt gac gga cgc aca gtc Leu Ile Thr Tyr u Leu lie Gu Gy Ala Arg Asp y Arg Thr Val 45 gca gac ctt atg agc tgg gga agc acc att ttg act agg gat gat gtc Ala Asp Leu IVet Ser Trp G y Ser Thr IIe Leu Thr Arg Asp Asp Val 60 tta gaa ggc atc cca gag atg atc cct gac atc cag gtt gaa gca acg Leu G u y lie Pro G u IVt lie Pro Asp Ile n Val u Ala Thr 75 80 ttt gat gac ggc acc aag ctc gtc acc gtg cac aat ccc atc cga Phe Asp Asp Gy Thr Lys Leu Val Thr Val His Asn Pro lie Arg 95 100 taacccttga tgtttttagg agt <210> 18 <211> 100 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 18 Leu His lie Thr Pro Arg G u n u Lys Leu Iet lie Val Val Ala 1 5 10 Page Al a Pr a As p Thr O31n As n Le u Al a Ar g As p O31u I Ie seqI i st car r ect ed. t xt Ar g Ar g Ar g Lys Asp Ar g O31y Leu Lys Leu Asn Hi s Ala Leu lieThr Ty r Ou Leu Ilie Ou Gy Ala Arg 40 Val Ala Asp Leu I'b t Ser Trp O31y Se r Thr I Ie Leu Val Leu (1u (1y Ilie Prao (1u I'b t I Ie Pro Asp I Ie 70 75 Thr Phe Asp Asp 03 y Thr Lys Leu Val Thr Val Hi s 90 <210O> 19 <21 1> 972 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (949) <223> RXA02278 <400> 19 accatt ct gc ct gact aat c t gcgcaccga t gat ggt tt g ccgccat gag gt gat gat gc aggact tgca ggaagcct aa gat aaggt ct t ggaat ggat atg aca caa acc caa 11! I'bt Thr 03 n Thr 03 n cca Pr a c aa O31n Ar g gcc Al a ac g Thr aag Ly s aac As n gcg Al a gga O31y cgt Ar g c ac Hi s ggt O31y aaa Ly s t at Tyr ggt O31y cgg Ar g 120 ct g Le u gt g ValI tt g Le u gga O31y aac As n ac a Thr aat As n gcc Al a cga Ar g gag O31u g at As p t ac Tyr gca Al a 75 ccg Pr a gct Al a acc Thr ct g Le u c aa O31n g at As p ct g Le u aaa Ly s c aa O31n gt g ValI t at Tyr ac c Thr tt t Ph e t cc Ser 45 ggc O31y gct Al a gga O31y ct g Le u cat Hi s 125 at c I Ie c ac Hi s gga O31y ggc O31y tt g Le u cca Pr a g aa O31u 110 c aa (31n g at As p 15 c ag O31n c ag O31n g at As p at c I Ie gcc Al a 95 t ac Tyr tt c Ph e g at As p ggt O31y gt t ValI gt g ValI ac c Thr ac g Thr tt g Le u ac c Thr 1 c aa O31n gcg Al a t gc cys t at Tyr ac g Thr c ag O31n gcg Al a aag Ly s gga O31y ct t Le u t ac Tyr gag O31u c aa O31n c ac Hi s g at As p gt g ValI 130 ccc Pr a cga Ar g ac c Thr c ag O31n t cg Ser ac c Thr c aa O31n 115 gcg Al a c aa O31n gt c ValI at c I Ie c aa O31n gcc Al a g aa O31u 1 00 ac c Th r ct g Le u Page 21 seqI i st car r ect ed. t xt ccg Pr o ccc Pr o 1 50 at c I Ie ct c Le u ac a Thr ct t Le u ggc O31y 230 at t I Ie g aa O31u gt g ValI gca A a ggc O31y g ac As p ccg Pr o t ca Ser 200 gct A a gt c ValI cgc Ar g at t I Ie tt g Le u 280 tt t Ph e c ac Hi s ac c Thr 1 70 t cc Ser c ag O31n ct g Le u tt c Ph e ct g Le u 250 t cg Ser gt g ValI tt t Ph e 155 aca Thr cga Ar g at g vb t aat As n t t a Le u 235 ag c Ser ct g Le u atg agc gaa I'b t Ser 03 u 140 gct t ac gat A a Tyr Asp ggg cga ct C G31y Ar g Leu gag gga agt O3 u 03 y Ser 1 90 at t gt g at g Ilie Val I'b t 205 gag caa ctt O3 u 03 n Leu 220 agc gcg ccg Ser Ala Pr o aac cgc act Asn Arg Thr ttg cgc ggg Leu Ar g G31y caa atc acc 03 n I I e Th r g aa O31u gt g ValI 1 75 tt t Ph e ggg G31y gcc A a ggc G31y gag O31u 255 c99 Ar g at g vb t 1 60 ct c Le u 999 G31y g aa O31u gcg A a ac g Thr 240 gag O31u t gg Tr p 145 cgt Ar g tt g Le u t gg Tr p ggc G31y c ac Hi s 225 tt a Le u tt g Le u cgc Ar g cca ggc Pro G31y ct a cac Leu Hi s gat aat As p As n acg gaa Thr 03 u 1 gt c gat Val As p 210 cct gat Pr o As p ct g cgc Leu Arg at t aac I I e As n ggg cag (31y (1n t gg Tr p ac c Th r tt a Le u 1 c ag O31n aag Ly s gt g ValI gga G31y ct g Le u 260 g aa O31u cgg aag t ac Arg Lys Tyr t agacggcgt cgagaaat cg aag <210O> <21 1> 283 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> I'bt Thr O31n Thr O31n Pr o Val O31y Th r 1 5 O31n O31y Pro O31n O31y O31n Se r Ar g Ala Ala Leu Ar g Val I Ie Arg Pro Hi s Ty r 40 Cys Tyr Thr I Ie I Ie Ala IIe O31y O31y Tyr O31u O31n O31n Phe Thr I Ie Lys Asp 70 Thr O31n Ser Ala Thr Lys I Ie Tyr Ar g O3 n Hi s Thr 03 u I I e As n Val 03 y 03 u 100 105 Leu Arg 10 Val O31u Leu Asp O3 y Ty r As n A a Thr Pr o 90 As n A a Page 22 Thr Ph e Ser O31y A a O31y Le u As p O31n O31n As p I Ie A a Ty r seqI i st car r ect ed. t xt Arg Ou Ala Thr Tyr His 120 125 Ala Asp On Thr Ilie Ala Tyr 115 03 n Phe Thr Ly s Thr 145 Ar g Le u Tr p O31y Hi s 225 Le u Le u Ar g Al a O31y Hi s As n O31u 1 95 As p As p Ar g As n O31n 275 Hi s Hi s O31u 1 65 Le u Tyr O31n Tyr Ph e 245 Hi s Pr o Ser 135 As p Thr Ar g Hi s ValI 215 Al a Al a Hi s As n Al a G31y As p Pr o Ser 200 Al a ValI Ar g I Ie Le u 280 Thr Lys5 Ser As p 1 G31y (31u As n Thr Al a 265 Ar g ValI Ph e 1 55 Thr Ar g vb t As n Le u 235 Ser Le u Tyr Ser Tyr Ar g G31y ValI 205 O31n Al a Ar g Ar g O31n O31u ValI 1 Ph e O31y Al a O31y G u 255 Ar g I Ie vb t 1 Le u O31y O31u Al a Thr 240 G u Tr p <210O> 21 <21 1> 594 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (571) <223> RXA02275 <400> 21 gtttgtcaac ggtgagttga tcaccagcaa gccagcagag ct act tct tg t tct aat ccg ccaacaagga aggaagat cc at c I Ie gt c ValI Ar g ggg O31y ac c Th r ggt O31y gca Al a ccg Pr o at c lIe cgc Ar g ccc Pr o ct g Le u t ac Ty r tt t Ph e gga O31y cgt Ar g g at As p g aa O31u gga O31y g aa O31u g aa O31u g at As p g aa O31u 45 ggt O31y ccg Pr a tt a Le u g aa O31u g at As p gag ttt O3 u Phe 15 gt t tt g Val Leu ct g ggg Leu 03 y gt g tt g Val Leu Page 23 acagt gccaa t ggcacgt cg at g at t at c act gcg 'b t Ilie Ilie Thr Al a 1 gt t gaa gga cgc gat Val O31u O31y Ar g Asp gat aag cgc at t caa Asp Lys Arg Ilie O31n t tg cgg t ta aac cac Leu Arg Leu Asn His aaa gct gat gat aag Lys Al a Asp Asp Lys acg gta ttt gtg gtg gag Thr Val Phe Val Val G u 75 cca agc gat att cac cag Pro Ser Asp lie His n agg cac ctg cca gca cag Arg His Leu Pro Ala G n 105 gca gcc atg atc gtg caa Ala Ala Met Ile Val n 120 gag cac ggc atc gag tat G u His G y lie G u Tyr 135 ttc agg cat agc ggg cac Phe Arg His Ser G y Hi s 150 155 <210> 22 <211> 157 <212> PRT <213> Corynebacter i um gl <400> 22 IVet Ile Ile Thr Ala lle 1 5 Val G u y Arg Asp Val Asp Lys Arg lie On Arg Leu Arg Leu Asn His y Lys Ala Asp Asp Lys Thr 70 Val Leu Val lie Thr Pro His Ser Leu G y Asn Arg 100 G u Leu Thr G u Lys Al a 115 Val Ser Phe Leu Asp G u 130 Val Pro Pro Ile Pro Phe 145 150 at t SIle at g Mt t t t ttt Phe t ac Tyr cag G n 140 aca Thr seql i st cor r ect ed. t xt atc ccc acg gat gtt tta gtt atc Ile Pro Thr Asp Val Leu Val lie gga ttt gtg gcg cac tcc ctg gga G y Phe Val Al a His Ser Leu G y 95 100 tcc aag cca ggt gaa ttg aca gag Ser Lys Pro y G u Leu Thr G u 110 115 gat cac acg gtg gtc agc ttc ttg Asp His Thr Val Val Ser Phe Leu 125 130 cgc acc gaa ctt gtt ccg cca att Arg Thr G u Leu Val Pro Pro Ile 145 cat tgatggatct tgacgctgat ttt Hi s ut am cum Asp Thr Asn I e G y Val Val Al a Leu 40 His Pro Ile Val Phe Val Ser Asp Il e His Leu Pro 105 Ala Iet Ile 120 Hi s G y I I e 135 Arg His Ser Tyr Phe Sy Ar g G u G n G n G n Tyr Hi s 155 G u Asp G u Sy SIle Sy Ser Asp 125 Ar g Hi s G u Val Leu Val Thr Val Pr o Thr G u <210> 23 <211> 801 <212> DNA <213> Corynebacter i um gl utam cum <220> Page 24 seal i st cor r ect ed. t xt <221> CDS <222> (101)..(778) <223> RXA02276 <400> 23 atcacacggt ggtcagcttc ttggatgagc acggcatcga gtatcagcgc accgaact t g ttccgccaat tcctttcagg catagcgggc acacacattg gat Asp ttt Phe acc Thr caa G n ccg Pr o act Thr at g Met t at Tyr gct Al a at c SIle 150 gtt Val gcc Al a gat Asp at g Met ct g Leu cac Hi s gca Al a acc Thr gt g Val gcg Al a gag G u gaa G u 120 gct Al a gct Al a ggc Gy cgt Ar g at t SIle 200 cat Hi s ttg Leu ttt Phe gag G u tct Ser gag G u gaa G u gt g Val at t SIle gcc Al a ct c Leu cca Pr o 170 cat Hi s ttc Phe acc Thr cat Hi s gga Gy tt c Phe gat Asp ct g Leu 75 at t SIle gct Al a caa G n acc Thr acg Thr 155 ct g Leu at c SIle tgt Cys caa G n tta Leu ttt Phe caa G n gct Al a aaa Lys cgg Ar g gaa G u cat Hi s cac Hi s 140 gca Al a ggg G y ggt G y tcg Ser cgc Ar g 220 tcg Ser gaa G u gac Asp 45 ttg Leu cgg Ar g gaa G u acc Thr cga Ar g 125 agc Ser acg Thr caa G n gcg Al a ggt G y 205 gca Al a gat Asp act Thr tgg Tr p gca Al a ct g Leu gct Al a t ac Tyr 110 gag G u gcg Al a gt g Val at g Met gcc Al a 190 gat Asp cga Ar g t ca Ser 15 t at Tyr ct g Leu at g Met gat Asp aat Asn 95 t cc Ser ct a Leu ggg G y agt Ser gca Al a 175 gt g Val cca Pr o ct a Leu gca Al a at g Met aaa Lys agg Ar g gag G u 80 gct Al a gt g Val t ca Ser at t SIle tcg Ser 160 ggt G y aaa Lys ggc G y ttt Phe atg gat ct t IVet Asp Leu 1 ctt cca acg Leu Pro Thr gat gca gag Asp Al a G u gtc ctg ctt Val Leu Leu atg ttt tac IVet Phe Tyr cgc ctt ttt Arg Leu Phe cga at g ggt Arg IVet G y ccc ctg att Pro Leu Ile 115 ggg cac ccg G y His Pr o 130 gat gtg gat Asp Val Asp 145 ct g at c caa Leu Ile G n cag cgg gtg Sn Arg Val cgt agc gcg Arg Ser Ala 195 ttg gat att Leu Asp Ile 210 atg agt taac Vet Ser 225 gac gct Asp Al a gga gcg G y Al a cga at c Arg Ile aag gtg Lys Val gcc acc Al a Thr gct gga Al a G y acg cgc Thr Arg 100 gtt gag Val G u gct ttg Al a Leu cga gca Arg Ala aat gcg Asn Ala 165 atg ttc IVet Phe 180 aac ttg Asn Leu tca caa Ser G n gaaggag aaaagaaaca tgg Page seql i st cor r ect ed. t xt <210> 24 <211> 226 <212> PRT <213> Corynebact eri um gl ut arri cum <400> 24 SIVet Asp Leu Asp Al a Asp Phe Leu Leu Leu Hi s Leu Ser Asp Ser Al a 1 5 10 Leu Pro Thr G y Ala Phe Ala His Ser Phe G y Phe G u Thr Tyr bet (C 20 25 O 00 Asp Ala G u Arg lle Thr Asn Ala G u G u Phe G n Asp Trp Leu Lys 40 C Val Leu Leu Lys Val G n Leu Thr Ser Ser Asp Ala Leu Ala IVet Arg 50 55 0 IVet Phe Tyr Ala Thr Pro Thr Val Ser G u Leu Lys Arg Leu Asp G u CK1 65 70 75 Arg Leu Phe Ala y Thr Pro Ala Arg G u lie Arg G u Ala Asn Ala 90 Arg IVet y Thr Arg IVet Ala G u Ile Val Ala G u Thr Tyr Ser Val 100 105 110 Pro Leu Ile Val G u Tyr Leu G u Leu lie n His Arg G u Leu Ser 115 120 125 G y His Pro Ala Leu Al a Leu Ala Leu Ala Thr His Ser Ala G y IIe 130 135 140 Asp Val Asp Arg Ala lle His Ala His Leu Thr Ala Thr Val Ser Ser 145 150 155 160 Leu ile n Asn Ala Val Arg y lie Pro Leu G y n IVet Al a y 165 170 175 G n Arg Val bet Phe Al a bet Arg G u His IIe G y Ala Ala Val Lys 180 185 190 Arg Ser Ala Asn Leu Asp G u Ile Asp Phe Cys Ser y Asp Pro y 195 200 205 Leu Asp Ile Ser G n IVet Val His G u Thr G n Arg Ala Arg Leu Phe 210 215 220 Met Ser 225 <210> <211> 738 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(715) <223> RXA02277 <400> attgatttct gttcgggtga tccaggcttg gatatttcac aaatggttca tgaaacccaa cgcgcacgac tatttatgag ttaagaagga gaaaagaaac atg ggt cca atc aga 115 Page 26 seqI i st car r ect ed. t xt [vb t (1y Pro I Ie Ar g at c Ar g aac As n gt g ValI c ac Hi s g ac As p ggt G31y t cc Ser gct Al a g ac As p 1 50 gcc Al a ac c Thr at g vb t gt a ValI ac g Thr at c I Ie cca Pr o gcg Al a gt g ValI g at As p t ac Tyr 120 c aa O31n gcc Al a gca Al a g at As p c ag O31n 200 ggg G31y gcg Al a ac c Thr g aa O31u cgt Ar g cgc Ar g ct c Le u at c I Ie at g vb t t at Tyr cgc Ar g 1 70 tt g Le u ccg Pr o ct t Le u at t I Ie cgc Ar g gaa O31u 75 t t c Ph e t ct Ser g at As p at t I Ie gt t ValI 155 aag Ly s g at As p gt c ValI at c I Ie g aa O31u at t I Ie g ac As p cca Pr o gca Al a gt t ValI aag Ly s 140 ggt G31y aac As n aag Ly s ggc G31y g ac As p g ac As p 45 gt t ValI ac c Thr g at As p ac g Thr gcc Al a 125 t cg Ser gcc Al a aaa Ly s gt c ValI gcc Al a 205 gcc Al a g aa O31u gcc Al a ggc G31y t cc Ser ct g Le u tt c Ph e 110 c aa O31n g at As p aac As n cca Pr o tt g Le u 1 gga G31y 15 gt c ValI aag Ly s at t I Ie at g vb t g aa O31u 95 t cg Ser ggt G31y tt g Le u ct a Le u tt c Ph e 1 75 g aa O31u aaa Ly s ag c Ser at t I Ie g aa O31u aat As n 80 ct c Le u cca Pr a gag O31u tt t Ph e g at As p 1 60 t gc cys t gg Tr p c ag O31n gct Al a gcc Al a gga O31y gca Al a tt t Ph e ct g Le u at c I Ie 130 at t I Ie at g vb t act Thr cgc Ar g ct g Le u gca Al a gcc Al a gga O31y gcg Al a gt g ValI gt g ValI 115 ccg Pr a aat As n gt g ValI aat As n cat Hi s gt a ValI at c I Ie aat As n t gc cys at c I Ie g aa O31u 1 00 g at As p ag g Ar g aaa Ly s g aa O31u ct g Le u 1 gag O31u gag O31u act Thr gga O31y cca Pr a aaa Ly s t ct Ser tt t Ph e aaa Ly s act Thr g at As p 1 cgc Ar g gt g ValI gac ttg cag gaa Asp Leu 03 n 03 u taaatgacac aaacccaacc agt <210O> 26 <21 1> 205 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 26 I'b t O31y Pro iieArg Ile OyVal Oy Oy PraVal Oy Aia(3y Lys 1 5 10 Thr O31n Leu Val O31u Arg lieThr Arg Ala Leu I Ie Asp O31u Val Ser 25 Page 27 seqI i st car r ect ed. t xt I'bt Al aAl a HIe Thr Asn Asp HIe Tyr Thr Il e 0 u Asp 40 A a Lys I I e Le u Thr As p I Ie (31u Ly s I Ie 145 ValI Le u I Ie A a G31y A a ValI ValI 115 Pr o As n ValI As n Hi s 1 95 G31y Pr o Ly s Ser Ph e Ly s Thr As p 1 65 Ar g ValI ValI Hi s 70 As p G31y Ser A a As p 150 A a Thr vb t Le u Thr Le u G31y I Ie G31y 135 Le u Ly s As p vb t Pr o A a ValI As p Tyr 120 O31n A a A a As p O31n 200 Ou le Ou As n 1 li1e O31y Pr o Ph e O31y 1 As p Ar g O31u Ph e Ser As p I Ie ValI 1 Ly s As p O31n ValI Thr As p Thr A a 125 Ser A a Ly s ValI A a 205 I Ie vb t (31u Ser O31y Le u Le u Ph e 1 (31u <210O> 27 <21 1> 1119 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1096) <223> RXA02603 <400> 27 gcat ggt gct t ggcgt gcat t acccaaccg at gt gct agc cgaccgcaga ggccgt ccat aagat cgaaa gggct acgaa gct A a ac c Th r gct A a gt t ValI aac cat Hi s gca A a ggc G31y gt g ValI cgc cgc Ar g g aa (31u at c I Ie gca A a at c g at As p gac As p aag Ly s gca A a at c ac c Thr aac As n gcg A a cca Pr a g ac c aa O31n aag Ly s ct g Le u 45 ct t Le u gt t aat As n aag Ly s cgc Ar g gct A a gct t tc tt a Phe Leu 15 cgc caa Ar g O31n ccc aag Pr o Lys gct ggt A a 03 y at c gca Page 28 cggcgcgt t g t t gggagcag gt g agc gaa cac gcc Val Ser O31u Hi s Ala 1 acc t cc gaa ccg cac Thr Ser 31u Pr o Hi s ccg ccg aaa aac ctt Pro Pro Lys Asn Leu cag t gg gt c aag aac O31n Tr p Val Lys Asn gca gat gcg at c tt c A a Asp A a Il e Phe t tc gt a gt g t tc t gc seql i st cor r ect ed. t xt Val Ala lie Ala Phe Val Asn On Arg Thr Ile lie Asp 75 Val Phe Cys ttc Phe gct Al a gt c Val ct a Leu tgc Cys 150 tgg Tr p at g Met cag G n gga G y at c SIle 230 tgg Tr p ttc Phe t cc Ser gac Asp gtt Val 310 gca Al a cgc Ar g cca Pr o 120 at c SIle at t SIle cac Hi s cgc Ar g ttc Phe 200 cgc Ar g aag Lys at g Met ctt Leu gtt Val 280 ggc Gy cag G n gcc Al a cac Hi s gga Gy ct g Leu gt g Val cca Pr o 170 at g Met ct a Leu gca Al a ct g Leu gca Al a 250 caa G n ttc Phe ggc G y ct a Leu at t l Ie cca Pr o at g Met tct Ser tac Tyr 155 gt g Val gca Al a gt c Val gaa G u gaa G u 235 aca Thr cac Hi s acc Thr ggt G y gcc Al a 315 t ac Tyr acc Thr gca Al a ttc Phe 140 at t SIle at c SIle ggt G y gct Al a at c SIle 220 agc Ser gca Al a t cc Ser at c SIle gcc Al a 300 ct a Leu ttg Leu aag Lys t ac Tyr 125 ct c Leu gcg Al a gat Asp ggt G y gcg Al a 205 ctt Leu t ac Tyr gt g Val acc Thr gcc Al a 285 cct Pr o gca Al a gtt Val cgt Ar g 110 ggc G y gcc Al a ct g Leu at t SIle gt c Val 190 ttt Phe ct g Leu acc Thr gt c Val gac Asp 270 at c l Ie gac Asp tgg Tr p aat Asn 95 ttc Phe at g Met acc Thr cag G n gcg Al a 175 gca Al a ggt G y cac Hi s ccc Pr o at g Met 255 gca Al a ct g Leu gaa G u gtt Val gat Asp cgc Ar g gcc Al a gac Asp ct g Leu 160 ctt Leu gca Al a t cc Ser gag G u acc Thr 240 t cc Ser ggt G y cgc Ar g gt g Val ttc Phe 320 gcc Al a ccc Pr o gt g Val ggc G y 145 gga G y gt c Val ggc G y ct g Leu cgc Ar g 225 t ac Tyr t ac Tyr ccg Pr o t ac Tyr gca Al a 305 tgc Cys cgt Ar g at c SIle gcg Al a 130 gt g Val t ac Tyr t cc Ser at c SIle ttc Phe 210 acc Thr ct g Leu gca Al a tgg Tr p gca Al a 290 ct c Leu at c SIle gac Asp gct Al a 115 ct c Leu gca Al a tgc Cys t cc Ser gag G u 195 at g Met ggc G y cgc Ar g ct g Leu t ac Tyr 275 gcc Al a agc Ser gt g Val gt g Val 100 gca Al a at t SIle ctt Leu ttc Phe gga G y 180 ct a Leu gca Al a gct Al a ttc Phe tgg Tr p 260 caa G n ggc G y gac Asp at g Met gaa G u gga G y gca Al a gcc Al a ggt G y 165 ttc Phe t cc Ser tct Ser aag Lys gtt Val 245 ggc G y at c SIe gt a Val aaa Lys gct Al a 325 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1119 gtg tac atc at g Val Tyr lie Vbet ccg atg ttt Pro IVet Phe 330 tgaatattta ccaatgaaca tgc Page 29 seqi i st car r ect ed. t xt <210O> 28 <21 1> 332 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 28 Val Ser Ou His Ala Ala Olu His His Thr Pr a O31n A a Ph e A a Pr a ValI G31y 145 G31y ValI G31y Le u Ar g 225 Tyr Tyr Pr a Tyr A a 305 O31u Ly s ValI A a ValI As p A a 115 Le u A a cys Ser O31u 1 95 vb t G31y Ar g Le u Tyr 275 A a Ser Hi s Le u As n Ph e cys O31u G31y A a A a G31y 1 65 Ph e Ser Ser Lys5 ValI 245 Gy le ValI Lys5 Thr A a ValI As n 70 Ph e A a ValI Le u Cy s 150 Tr p vb t O31n Oy le 230 Tr p Ph e Ser As p ValI 310 Thr As p Le u O31n G31y As p Le u Ser 135 ValI Lys5 Le u Tr p Lys5 215 Ar g Thr As p vb t Thr 295 Le u A a G31y 40 ValI Ar g A a Ar g Pr a 120 le le Hi s Ar g Ph e 200 Ar g Lys5 vb t Le u ValI 280 G31y O31n lIe vb t Le u Thr Ser O31u 1 05 ValI O31y O31y vb t A a 1 85 Le u Tyr Ser A a Ser 265 Pr a As p ValI Ar g 10 Ou le A a I Ie A a 90 Hi s O31y Le u ValI Pr a 1 70 vb t Le u A a Le u A a 250 O31n Ph e O31y Le u Asp Thr 03 n Asn Phe Leu As p Ly s A a I Ie li1e Pr a vb t Ser Tyr 1 ValI A a ValI O31u O31u 235 Th r Hi s Th r O31y A a 315 As n A a Pr a As p Tyr Thr A a Ph e 140 le le Oy A a I Ie 220 Ser A a Ser I Ie A a 300 Le u Ly s Le u Le u ValI Le u Ly s Tyr 125 Le u A a As p O31y A a 205 Le u Tyr ValI Thr A a 285 Pr a A a Ly s Ar g A a A a ValI Ar g 110 O31y A a Le u I Ie ValI 1 Ph e Le u Thr ValI As p 270 li1e As p Tr p Ar g Pr a A a I Ie As n Ph e vb t Thr O31n A a 1 A a O31y Hi s Pr a vb t 255 A a Le u O31u ValI Cys I Ie Val N/t Ala Val Tyr I Ie N/t Pro N/t Phe 325 330 Page seqi i st car r ect ed. t xt <210O> 29 <21 1> 2004 <21 2> DNA <21 3> COr ynebact er i urn gi ut arri cumn <220> <221 CDS <222> (1981) <223> RXA01385 <400> 29 at cacact tt cat tcgcagt gt gat ct gaa ct acat t tct t ccgt gaat g agat aggaaa t cccct cgaa aggaccagac ggttactgta cggaacacgc atg cag ttt cat tat 11! l'bt O31n Phe Hi s Tyr g aa O31u gga G31y gt t ValI tt c Ph e g aa O31u c ag O31n ac c Thr at t I Ie ccg Pr o tt c Ph e 1 50 tt c Ph e ac c Th r t ac Tyr aac As n gca A a aat As n gcc A a tt t Ph e at g vb t g ac As p 120 gcg A a aag Ly s g ac As p cgc Ar g ac c Thr ccg Pr o ccg Pr o ac c Thr gca A a tt c Ph e tt c Ph e ag c Ser ct c Le u t cc Ser g aa O31u 1 70 ac c Thr ggt O31y gac As p gct A a acc Thr g at As p 75 gcc A a t gg Tr p acc Thr acc Thr cca Pr o 155 gt a ValI ag t Ser g ac As p g at As p ggc O31y cgc Ar g ggc O31y c ac Hi s aag Lys5 cgc Ar g c ag O31n 140 ac c Thr g aa (31u G31y cca Pr a ct c Le u ac c Thr 45 ct c Le u gt g ValI gag (31u ccg Pr a gag (31u 125 ac c Thr ag g Ar g g aa (31u gag (31u at g vb t ccc Pr a at c I Ie gt a ValI c ac Hi s at c I Ie g ac As p 110 ct g Le u cgc Ar g at g vb t g ac As p c aa O31n 1 90 gag O31u 15 gag O31u gca A a gag O31u t cc Ser ct c Le u 95 ccg Pr a cca Pr a at c I Ie aag Lys5 gca A a 1 75 act Thr at g vb t gt c ValI gcg A a ag a Ar g cga Ar g gcc A a c aa O31n c ag O31n at c I Ie cct Pr a 1 g aa O31u ggc (31y 1 cgc Ar g at g vb t gct A a ag c Ser ac c Thr g aa O31u aac As n c ac Hi s g ac As p 145 g ac As p t at Tyr g aa (31u gcg A a g at As p c ag O31n g ac As p at t I Ie gct A a cct Pr a at c I Ie 130 c ac Hi s t at Tyr ccg Pr a tt g Le u g aa O31u gt t ValI ct t Le u cgt Ar g g aa O31u cat Hi s cgt Ar g 115 agt Ser tt c Ph e gga O31y gt a ValI gt c ValI 1 ggt O31y ct c Le u t cc Ser cgc Ar g act Thr g aa O31u 1 00 gag O31u g aa O31u aac As n t ac Tyr at t I Ie 1 ac c Th r cga acc aag t ac ct g gt c ggt gcc gat ggt gca cga agc caa gt g cgc Page 31 Arg Thr Lys 200 Tyr Leu Val G y Al 20 seql i st cor r ect ed. t xt a Asp G y Ala Arg Ser G n Val Arg 5 aaa Lys ggt Gy 230 aag Lys cgt Ar g cct Pr o at c SIle aac Asn 310 cat Hi s at t SIle cag G n ctt Leu t ac Tyr 390 aag Lys gcc Al a ggc Gy aac Asn ct g Leu at g Met acc Thr ggt Gy gat Asp 280 acc Thr gt g Val gat Asp at t SIle at g let 360 cat Hi s gaa G u tgg Tr p gac Asp at g let 440 gat Asp t ac Tyr at t SIle aaa Lys 250 ttc Phe agc Ser aac Asn aac Asn cgt Ar g 330 ggc G y gt g Val gcc Al a cgc Ar g aca Thr 410 gag G u cac Hi s gca Al a cga Ar g cac Hi s 235 tct Ser ct c Leu aag Lys cag G n t cc Ser 315 gtt Val gac Asp t cc Ser agc Ser gaa G u 395 ct c Leu gat Asp t at Tyr aag Lys ct c Leu 220 gcg Al a gat Asp ttc Phe gct Al a at c SIle 300 at c SIle t ca Ser gcc Al a at g Met gga G y 380 gac Asp at g Met ttc Phe cct Pr o ggc G0y 460 caa G n aac Asn tcg Ser cgt Ar g gtt Val 285 at g bet t ac Tyr gaa G u tgc Cys cag G n 365 aat Asn at t SIle gca Al a t ac Tyr cct Pr o 445 t ac Tyr ggt G y acc Thr ggt G y ct c Leu 270 cgt Ar g gct Al a gag G u aaa Lys cac Hi s 350 gac Asp agc Ser gcc Al a aag Lys cgc Ar g 430 tct Ser cca Pr o aag Lys gag G u cgc Ar g 255 t ac Tyr gat Asp cca Pr o gt a Val acc Thr 335 acc Thr gga G y cca Pr o t ac Tyr cca Pr o 415 gcg Al a t cc Ser at t SIle cag G n ttc Phe 240 acc Thr gtt Val acc Thr ttc Phe ggc G y 320 tcg Ser cac Hi s ttc Phe cgc Ar g aag Lys 400 agc Ser aac Asn at c SIle ggc Gy gct Al a 225 ccc Pr o at c SIle gac Asp cca Pr o acc Thr 305 cac Hi s agc Ser agc Ser aac Asn gaa G u 385 ct c Leu agc Ser tct Ser aca Thr cga Ar g 465 210 aac Asn gac Asp ttg Leu ct g Leu ct c Leu 290 ct c Leu cgc Ar g gaa G u gct Al a ctt Leu 370 ct a Leu at c SIle gaa G u gag G u at g Met 450 cgc Ar g gct Al a cgc Ar g at c SIle 260 gaa G u gat Asp gt g Val gca Al a cca Pr o 340 gct Al a tgg Tr p cag G n t ac Tyr ggc G y 420 aat Asn ggc G y aag Lys tgg Tr p aag Lys 245 cca Pr o gt a Val gt c Val aaa Lys gac Asp 325 cgc Ar g ggc G y aag Lys acc Thr gac Asp 405 agt Ser gcc Al a agc Ser t ca Ser 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 gcg atg gtt ggt cga gtc tgc gac ttc acc gaa aca cac ctc ggt cac Page 32 Ala MVt Val Gy Arg Val 470 475 Cys As seql i st cor r ect ed. t xt p Phe Thr G u Thr Hi s Leu G y Hi s caa G n gat Asp gca Al a caa G n aaa Lys 550 ggt Gy gat Asp ttc Phe ttc Phe aca Thr ctt Leu gcg Al a 520 cct Pr o gca Al a acc Thr gt c Val ct c Leu 600 aaa Lys gac Asp 490 ggc Gy ctt Leu acc Thr ggg Gy gac Asp 570 gt g Val gat Asp aaa Lys gga G y gag G u gac Asp gag G u at c SIle 555 t cc Ser gt a Val acc Thr ggc G y cgc Ar g ggt Gy ccc Pr o ct c Leu 540 ttc Phe gac Asp gt c Val caa G n gca Al a 620 at g Met tct Ser acg Thr 525 gac Asp gaa G u at c SIle cga Ar g ggg G y 605 cat Hi s cgc Ar g gag G u 510 ctt Leu acc Thr ct g Leu ttt Phe cca Pr o 590 ctt Leu cag G n gca Al a 495 ct a Leu gt c Val cgc Ar g acc Thr gat Asp 575 gac Asp ggc G y ct a Leu 480 t ac Tyr gac Asp gac Asp cag G n aat Asn 560 agt Ser caa G n gaa G u at c SIle ttc Phe tgg Tr p aag Lys 530 cca Pr o gaa G u gag G u gtt Val ct c Leu 610 gcg Al a gca Al a gca Al a 515 gt g Val t cc Ser aac Asn at c SIle t cc Ser 595 acc Thr aac Asn gga G y 500 gaa G u at t SIe gt g Val t cc Ser t cc Ser 580 gga Sy gga G y 1603 1651 1699 1747 1795 1843 1891 1939 1981 2004 taaggcacag ctgttaaaac agt <210> <211> 627 <212> PRT <213> Corynebacter i um gl ut ai cum <400> IVet G n Phe His Tyr G u y Tyr Ala 1 5 Arg Ala u y Ser y lie Asn Arg Met Asp Val Leu Ile Val y Ala y 40 Ala G n Leu Ser Arg Phe Pro Asn Val Ser Asp Arg Arg Leu G u Leu Ala Asn 70 Thr Ile u Thr Phe n Ala Phe y G u Ala His G u Ile Thr Asp M\t Al a 100 105 Thr G y 10 Pro Asp Pro Al a Thr Thr Al a Asp 75 Phe Al a 90 Phe Trp Page 33 Pr o Leu Thr Leu Val G u Pr o G u G u Al a G u Ser Leu Pr o bet Val Al a Ar g Ar g Al a G n seqI i st car r ect ed. t xt Asn Pro Arg Ou Ilie lieArg Asp 115 120 Asn Ser Thr Ar g O31u Leu Pro O31n 125 Hi s As p 145 As p Tyr O31u Ar g A a 225 Pr o I Ie As p Pr o Thr 305 Hi s Ser Ser As n O31u 385 Le u Ser Ser Thr Ser Ph e O31y ValI ValI 1 95 O31n Hi s ValI Le u O31y 275 O31n As p ValI Hi s Ly s 355 G31y Le u (31u vb t Ph e 435 As p Ph e Ar g (31u 1 65 ValI ValI Ar g Tr p Ly s 245 Pr o ValI ValI Ly s As p 325 Ar g G31y Ly s Thr As p 405 Ser A a Ser Pr o Ph e 150 Ph e Thr Ar g Ly s G31y 230 Ly s Ar g Pr a I Ie As n 310 Hi s I Ie O31n Le u Tyr 390 Ly s A a O31y As n vb t 135 vb t ValI Le u Thr Ser 215 ValI cys O31u As p As p 295 ValI Ph e Ph e O31y O31y 375 A a As n O31n Tyr O31n 455 A a Ly s As p Ar g Ly s 200 Le u vb t Thr O31y As p 280 Thr ValI As p I Ie vb t 360 Hi s O31u Tr p As p vb t 440 As p Le u As n Ph e Ar g 1 85 Tyr O31y As p I Ie O31y 265 O31y A a Tr p As p A a 345 As n ValI O31u Ser Le u 425 Thr Le u Le u Ser O31u 1 70 Thr Le u Tyr I Ie Ly s 250 Ph e Ser As n As n Ar g 330 O31y ValI A a Ar g Thr 410 O31u Hi s A a Thr Pr a 1 ValI Ser ValI Ar g Hi s 235 Ser Le u Ly s O31n Ser 315 ValI As p Ser Ser O31u 395 Le u As p Tyr Ly s Thr Ar g O31u O31u A a 205 O31n As n Ser Ar g ValI 285 vb t Tyr O31u cys O31n 365 As n I Ie A a Tyr Pr a 445 Tyr Ar g vb t As p O31n 1 As p O31y Thr G y Le u 270 Ar g A a O31u Ly s Hi s 350 As p Ser A a Ly s Ar g 430 Ser Pr a I Ie Ly s A a 1 Thr O31y Ly s O31u Ar g 255 Tyr As p Pr a ValI Thr 335 Thr O31y Pr a Tyr Pr a 415 A a Se r I Ie Ar g Ar g Phe Lys Ser Ala I'bt Val O31y Ar g Val Page 34 Cys Asp Phe Thr 03 u seql i st cor r ec 465 470 475 Thr His Leu G y His G n Ala Thr Ala Asp G y 485 490 Val Phe Al a G y Ser Asp Al a Leu Asn G y G u 500 505 Arg Trp Ala G u Trp Al a G u Ala Asn Leu Asp 515 520 Ala Lys Val lie Tyr G n Ser Pro Tyr Thr G u 530 535 Val Pro Ser Val Phe Lys Pro Ala Val Gy Ile 545 550 555 Val G u Asn Ser Phe G y Ile Thr Thr Asp Ser 565 570 Arg G u Ile Ser Arg Asp y Val Val Val Val 580 585 Tyr Val Ser Gy lie Phe Pro Leu Thr Asp Thr 595 600 Phe Leu Thr G y Tyr Phe Pro Lys IVet Lys G y 610 615 Asn Al a Asn 625 <210> 31 <211> 915 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(892) <223> RXN00675 <400> 31 gtggaagtac tcacctcaag gtggccggaa ccgcatgcat tggttttaac agtttaattt gaagaaagag acgtggaagc ted. txt Arg IVet G y Ser Pro Thr 525 Leu Asp 540 Phe G u Asp Il e Val Arg G n y 605 Al a Hi s 620 Al a 495 Leu Val Ar g Thr Asp 575 Asp Gy Leu aag Lys cag G n gag G u cag G n ggt G y aag Lys gcg Al a aaa Lys at c SIle ccg Pr o at t I e gag G u ggc Sy gat Asp t ca Ser gcg Al a at c SIle at g bet gct Al a gt g Val gca Al a gt c Val agc Ser ggc Sy tgc Cys aag Lys ggc Sy acg Thr 45 gcc Al a gct Al a acc Thr aag Lys tgg Tr p gtt Val tcg Ser gcc gct Al a Al a 15 gct ttg Al a Leu gat ctg Asp Leu cct aca Pro Thr gt c aat Val Asn Page tgtgcggtgc cggccacctt atg ggt ttc cgt tcc IVet y Phe Arg Ser 1 gag ctg gac gcg atg G u Leu Asp Ala IVet cag gct gtg cgc gct G n Ala Val Arg Ala gat cag atc gcg gag Asp G n Ile Ala G u ttc ctg ggt tac cag Phe Leu G y Tyr G n gag gtg att gtt cac G u Val Ile Val His seqI i st car r ect ed. t xt ggc G31y at c I Ie ag c Ser gct Al a aac As n 1 50 gct Al a gga G31y gag O31u gcc Al a g aa O31u 230 t gg Tr p ccg Pr o cca t cc aag Pr o Ser Lys t gc ggc gca Cys a3 y Al a 1 05 ggc at c ggc 31y Ilie G31y 120 gag t gg gt c O31u Tr p Val t tg acc gat Leu Thr Asp t cc aag tt c Ser Lys Phe 1 70 ggc at t ggc 31y Ilie G31y 1 85 aag gcc ggc Lys Al a G3 y 200 gag cct at g O31u Pr o I'b t gat t gg act Asp Trp Thr cac acc gtt Hi s Thr Val 250 t at t aaaat g Ty r ac c Th r tt t Ph e ct g Le u at g vb t 140 t cc Ser gt c ValI c ac Hi s ggc G31y ac c Th r 220 gt g ValI gcc Al a at c I Ie g at As p g ac As p 125 g aa O31u c ac Hi s gcg Al a at g vb t cct Pr o 205 ct c Le u act Th r ac c Th r tt g Le u ggt G31y 110 gag O31u G31y gct Al a ct c Le u c ac Hi s 1 90 gt g ValI ggc G31y ct c Le u aag Ly s g aa O31u gt c ValI gt c ValI aag Ly s gag O31u 1 at c I Ie gag O31u c ag O31n g aa (31u ggt G31y 240 ggc G31y ggc G31y ggc G31y c ag O31n gcc Al a 145 gt c ValI gt c ValI cca Pr o gag O31u g at As p 225 t ca Ser ccg Pr o g at As p g at As p ggt G31y 130 at g vb t gca Al a g at As p t ac Ty r ggc G31y 210 t cc Ser t gg Tr p cgc Ar g ct g Le u t cc Ser 115 ct c Le u gt t ValI ac c Th r ggc G31y Le u 1 t cc Ser gca Al a gca Al a at c I Ie gt g ValI 1 00 gcg Al a aac As n cca Pr a cgc Ar g t ac Ty r 1 gct Al a gt g ValI gt g ValI t ca Ser ct c Le u 260 at g ctt t tcgacg cat <210O> 32 <21 1> 264 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 32 b t (1y PheArg Ser Lys Lys LysVal lleAlaAla Lys Thr AlaAla 1 5 10 O31u L e uAs pAl a 'bt 31n Al aAl a (1y (1u I Ie Val G31y Lys Al aLeu 25 O31n Al a ValI Ar g Al a Ou Ala Lys Ala G31y I'bt Ser Thr Trp Asp Leu 40 Asp (31n I Ie Al a 31u O31n Val I IIe Ar g As p Al a G31y Ala Val Pro Thr 55 Page 36 seqI i st car r ect ed. t xt Phe Leu G3 y Tyr G3 n Ph e Pr o Ala Ser Val Cys Ala Ser Val Asn G31u G31y G31y (31n A a 145 ValI ValI Pr o G31u As p 225 Ser Pr o I Ie Le u Ser 115 Le u ValI Thr G31y Le u 1 95 Ser A a A a I Ie Val His G1y liePro Val Ser I Ie Asp Cys 1 00 Ala Tr p Ser Phe G31y 120 As n Leu A a Thr 03 u 135 Pro G31y As n Ar g Leu 150 Ar g Lys Ala G31u Ser 1 65 Ty r 03 y 03 y Hi s 03 y 1 80 A a As n G u G y Lys 200 Val Leu Ala I Ie G31u 215 Val Leu 31u As p As p 230 Ser Hi s Trp G31u Hi s 245 Leu Thr Pr o Ar g Ty r 260 Ser G31y 1 li1e Tr p Th r Lys5 I Ie 1 A a Pr o Tr p G31u Th r G31u Le u ValI 1 G31y Ar g Ly s Le u ValI 235 I Ie As p As p 125 G31u Hi s A a vb t Pr a 205 Le u Th r G31u Tr p As p vb t Le u G31y 1 Gu le Th r As p Thr Val Ala Ala Thr Lys 250 G31y (1y 255 <210O> 33 <21 1> 506 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (483) <223> FRXA00675 <400> 33 t gc ggc gca acc t tt gat ggt t gg gt c Cys G31y Ala Th r Phe Asp Gy Trp Val 1 5 ggc at c ggc gag ct g gac gag gac gt c O3 y Il e 03 y 03 u Leu Asp 03 u Asp Val 25 gag t gg gt c ct c at g gaa ggc at g aag G31u Tr p Val Leu 'b t G31u G31y I'b t Lys 40 t tg acc gat gt c t cc cac gct ct c gag Leu Thr Asp Val Ser Hi s Ala Leu G31u 55 ggc gat t cc gcg t gg agc tt c G31y Asp Ser Ala Tr p Ser Phe 10 cag ggt ct c aac t tg gct acc O3 n 03 y Leu As n Leu A a Thr gcc at g gt t cca ggc aac cgt Ala I'b t Val Pro G31y Asn Ar g gt c gca acc cgc aag gct gag Val Ala Thr Ar g Lys Ala G31u Page 37 IND seqI i st car r ect ed. t xt Stcc aag ttc ggc gtc gcg ctc ggc atc gtc gat ggc tac ggc gga cac 240 Ser Lys Phe G31y Val Al a Leu O31y I Ie Val Asp G31y Ty r 31y G31y Hi s 70 75 fl ggc att ggc cgc cac atg cac gag gag cca tac ttg gct aat gag ggc 288 31 Gy IlIe 1y Ar g Hi s N/bt Hi s (1u (1u Pr oTyr Leu Ala Asn (1u (1y 90 aag gcc ggc aag ggc cct gt g at t cag gag ggc t cc gt g ct c gcc at t 336 Lys Al a G31y Lys G31y Pr o Val Ilie O31n 31u G31y Ser Val Leu Al a Ilie 100 105 110 C1 gag cct at g ct c acc ct c ggc acc gaa gat t cc gca gt g ct g gaa gat 384 (3 OuPr o /b tLeu Thr Leu (1y Thr (1u Asp Ser Al aVal Leu (1u Asp 00 115 120 125 Sgat t gg act gt c gt g act ct c gac ggt t ca t gg gca t ca cac t gg gag 432 C1 Asp Tr p Thr Val Val Thr Leu Asp G31y Ser Tr p Al a Ser Hi s Tr p (31u ID130 135 140 Scac acc gtt gca gcc acc aag ggc ggc ccg cgc atc ctc acg ccg cgt 480 C1 Hi s Thr Val Al a Al a Thr Lys 31y G31y Pro Arg Ilie Leu Thr Pr o Ar g 145 150 155 160 t at t aaaat gat g ctt t tcgacg cat 506 Ty r <210> 34 <211> 161 <212> PRT <213> Cor ynebact er i urn gi ut ani cumn <400> 34 Cy s G31y Ala Thr Phe Asp Gy Trp Val Gy Asp Ser Ala Trp Ser Phe 1 5 10 G31y I Ie 31y (31u Leu Asp Ou Asp Val O31n G31y Leu Asn Leu Ala Thr 25 31u Tr pVal Leu 'b t O31u (1y /b t Lys Al a 'b t Val Pr o 1y As nAr g 40 Leu Thr Asp Val Ser Hi s Al a Leu O31u Val Al a Thr Arg Lys Ala Ou 55 Ser Lys Phe G31y Val Ala Leu G31y Ilie Val Asp G31y Tyr G31y G31y Hi s 70 75 G31y Ile(1y Ar g Hi s I'b t Hi s O31u (1u Pr oTyr Leu Al aAs n (1u (1y 90 Lys Al a G31y Lys G31y Pr o Val Ilie O31n 31u G31y Ser Val Leu Al a I Ie 100 105 110 (31u Pr o N/b t Leu Thr Leu G31y Thr (31u Asp Ser Al a Val Leu (31u Asp 115 120 125 Asp Tr p Thr Val Val Thr Leu Asp G31y Ser Tr p Al a Ser Hi s Tr p O31u 130 135 140 Hi s Thr Val Al a Al a Thr Lys G31y G31y Pr o Ar g Ilie Leu Thr Pro Arg 145 150 155 160 Ty r Page 38 seql i st cor r ect ed. t xt <210> <211> <212> <213> <220> <221> <222> <223> 996 DNA Cor ynebact eri um gl ut ai cum CDS (973) RXA01609 <400> gttgcagtca gtgaagctga agagcagcca gtcggctaaa gctttacctg tcatcgcttc aggcgactag catggtgacc aaatagcctc catccaaccc at g Met 1 t ct aaa at g Ser Lys IVet gca cca ctt gta Ala Pro Leu Val ccc Pr o gga att cct acc Gy Ile Pro Thr cca Pr o 15 atc agg gaa gta lie Arg Gu Val cct gca Pro Al a cat att gaa His Il e G u atc ggt gag lie G y G u cca gaa tat gtg Pro G u Tyr Val tgg Tr p aag gac gaa gtc Lys Asp G u Val caa gaa gca G n G u Al a aag atg cgt Lys IVet Arg cct ttt gtg cag Pro Phe Val G n gcc Al a 45 cct gag gtc atc Pro u Val lle gag Gu gag aca G u Thr tct cgc atc gct Ser Arg Ile Ala aac tca ctg aaa Asn Ser Leu Lys gcg ggc gaa gcc Al a G y u Al a gt c Val aag cca ggc gt g Lys Pro y Val act gat gaa ctt Thr Asp G u Leu gat Asp cgc att gtg cat Arg IIe Val His tac acc tgc gat Tyr Thr Cys Asp at g Met ggc gca tac cct G y Ala Tyr Pro t ca Ser 95 gat ctt ggt tac Asp Leu G y Tyr cgg gga Arg G y 100 ttc acc aag Phe Thr Lys att cct gat lie Pro Asp 120 t ca tgc att t cc Ser Cys Ile Ser ct c Leu 110 aat gag atc gtg Asn G u Ile Val t gc cac ggt Cys Hi s G y 115 aac atc gat Asn Ile Asp tcc acc gtc att Ser Thr Val lle gaa G u 125 gag ggc gat att Su G y Asp Ile gtt Val 130 gtc acc Val Thr 135 gcg ttc aag cac Ala Phe Lys Hi s ggc Sy 140 gtc cac ggc gac Val His G y Asp aat gcc acc ttc Asn Al a Thr Phe gcg ggt gat gtt Al a G y Asp Val gaa gaa cac cgc G u G u His Ar g ctg ctg gtt Leu Leu Val 160 gag cgc acc G u Arg Thr 165 gaa gaa gcc at g G u G u Al a Met at g Met 170 cgt tcc atc cgt Arg Ser lie Arg gca Al a 175 gca aag cct gga Ala Lys Pro y cgt gaa Arg G u 180 at c aac gt c Ile Asn Val ggg cgt gtc att G y Arg Val lie gag a u 190 tct tac gcc aag Ser Tyr Ala Lys cgt ttt ggc Arg Phe G y 195 tac aac gtg gtc cgc gat ttc acc gga Tyr Asn Val Val Arg Asp Phe Thr y cac ggc atc ggc cca act ttc His G y lie G y Pro Thr Phe Page 39 200 cac aac ggc ctt gtg gtg ct His Asn G y Leu Val Val L 215 2 ctg ctc gtg cca ggc atg a Leu Leu Val Pro Gy bet T\ 230 235 ggt tcc ctc gac tac gag at Sy Ser Leu Asp Tyr Gu Il 250 gtt gac cgt aag ttc agc g Val Asp Arg Lys Phe Ser Al 265 gaa gac ggc aat gag atc ct G u Asp G y Asn G u Ile L 280 taaaaacgcc taggccacaa gcc <210> 36 <211> 291 <212> PRT <213> Corynebacteri um gl ut :g eu hr c e cg a c eu seql i st cor r ect ed. t xt 205 210 cac tac gac aac act cag His Tyr Asp Asn Thr G n 225 ttg acc atc gag cca atg Leu Thr lie G u Pro IVet 240 tgg gaa gat gat tgg act Trp G u Asp Asp Trp Thr 255 cag ttc gag cac acc att Sn Phe u His Thr lie 270 acc ctc cca gac gat tcc Thr Leu Pro Asp Asp Ser 285 290 cgc gat Arg Asp aac ctt Asn Leu 245 caa aac Sn Asn 260 atc acc Ile Thr amn cum <400> 36 et Ser 1 Arg G u Su Val Ile G u I e Al a Arg Ile Leu G y Ile Val Ile Val 130 Cys Asn 145 Leu Val Lys Pro Lys IVet Val Pr o (n Gu Lys IVet (3 y G u Val Hi s Tyr Arg 100 Cys Hi s 115 Asn Il e Al a Thr G u Arg 3 y Arg 1 n Ar g 5 Al a Al a Ar g Al a G u Sy Sy Asp Phe Thr 165 G u Pr o l Ie Gy Thr 55 Lys Thr Thr Pr o Thr 135 Al a a u Asn Leu G u Gu 40 Ser Pr o Cys Lys Asp 120 Al a Sy Al a Val Val Ar g Pr o Ar g Sy Asp Ser 105 Ser Phe Asp Met SIle 185 Sy G u Val Al a Thr Sy Cys Val Hi s Ser 155 Ar g Ar g SIle Tyr Gn Al a Thr Al a SIle SIle Sy 140 G u Ser Val Pr o Val Al a Asn Asp Tyr Ser G u 125 Val Su I e I e Pr o Lys Gu Leu Leu Ser Asn Sy Sy Ar g Al a 175 Ser Ala Lys Arg Phe y Tyr Asn Val Val Arg Asp Phe Thr y His y Page 195 seql i st cor r ec 200 lie Gy Pro Thr Phe His Asn Gy Leu Val Val 210 215 Thr G n Tyr Arg Asp Leu Leu Val Pro G y Iet 225 230 235 Pro IVet Ile Asn Leu G y Ser Leu Asp Tyr G u 245 250 Trp Thr Val G n Asn Val Asp Arg Lys Phe Ser 260 265 Thr Ile Val lie Thr u Asp y Asn u Ile 275 280 Asp Ser Val 290 <210> 37 <211> 1644 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1621) <223> RXA01358 <400> 37 gagggtaaac cttatactcc gattcgtttt gatgtggtgg acggggcgcc cagagatcac tgtttatgag gatgtggaac ted. txt 205 Leu Hi s 220 Thr Leu lie Trp Al a G n Leu Thr 285 Tyr Asp Asn Thr Ile u 240 G u Asp Asp 255 Phe G u Hi s 270 Leu Pro Asp caatcgtttt ggatccgcac act Thr gag G u gca Al a caa G n tcg Ser acc Thr cat Hi s ttg Leu t cc Ser aat Asn act Thr agt Ser gct Al a gca Al a cag G n cct Pr o gcg Al a ggc Gy at t SIle ttg Leu at g Met ct c Leu acg Thr act Thr caa G n cca Pr o t ca Ser at g bet cgt Ar g 75 gtt Val tta Leu gga G y tta Leu gga G y gaa G u tgg Tr p aaa Lys gcc Al a ttt Phe gtt Val ggt G y ttg Leu tct Ser 45 cca Pr o caa G n cat Hi s ct g Leu ctt Leu gt g Val cct Pr o cgg Ar g aag Lys ggt Sy ggc Sy ggt Sy 110 cca Pr o gca Al a acc Thr cgt Ar g aaa Lys cag G n 80 aac Asn gt g Val ct g Leu atg gcg Vet Al a 1 aaa at c Lys II e tac att Tyr Ile att aaa I e Lys gt g at c Val lile tgt gat Cys Asp ccc aaa Pro Lys gcg ctt Al a Leu ct c ggt aga Leu G y Arg gtt cgt gtg Val Arg Val gtt gga tta Val G y Leu act gcg gtg Thr Al a Val att aac ct c Ile Asn Leu ctc gcg atg Leu Al a IVet gcg aag ttt Ala Lys Phe 100 gat gga acc Asp G y Thr 115 ttg gcc gcg aag gag Leu Ala Ala Lys G u Page 41 NO seql i st cor r ect ed. t xt 120 125 130 gaa ggt att ggc aag att gtg atc ccg gag gga aat gcc caa gaa gca 547 Su G y Ile G y Lys Ile Val Ile Pro u y Asn Ala 3 n u Al a ,C 135 140 145 4 gga ctg gtt gag gat cct tca gtc ttt ttg gca cat tcc atc gac cag 595 G3 y Leu Val 3 u Asp Pro Ser Val Phe Leu Ala His Ser Ile Asp O n 150 155 160 165 gtc ttg cga tgg ctt gat ggg gaa gag gcg ttg cct cag cct gga cta 643 Val Leu Arg Trp Leu Asp y u u Ala Leu Pro n Pro G y Leu C1 170 175 180 00 ttc aat gat gaa aat agc ctc aaa ctg cct gat atg cgt gat gtg gtg 691 SPhe Asn Asp u Asn Ser Leu Lys Leu Pro Asp 'b t Arg Asp Val Val 185 190 195 .C gga caa cca gaa gct agg t t t gct gca gaa gta gct gct gcc ggt ggt 739 (3 y 3 n Pro 3 u Ala Arg Phe Ala Ala 3 u Val Ala Ala Ala 3 y 3 y 200 205 210 cac cac atg ctg atg att ggc cct ccc ggt tct gga aaa tcc atg atc 787 His His 'b t Leu 'b t IIe G y Pro Pro G y Ser G y Lys Ser 'b t IIe 215 220 225 gcc gag cgg att ccc agc ttg ctt ccc gaa ctg tcg ccc caa caa atg 835 Ala u Arg Ile Pro Ser Leu Leu Pro u Leu Ser Pro n O n 'b t 230 235 240 245 atc gag gcg acg gca gtg cat tcc gtt gtg ggg cga acc ttt tca ggg 883 Ile O u Ala Thr Ala Val His Ser Val Val G y Arg Thr Phe Ser G y 250 255 260 ccg gtg tcg agg gct ccg ttt att tcc cca cac cac aat gtc agc aag 931 Pro Val Ser Arg Ala Pro Phe Ile Ser Pro His His Asn Val Ser Lys 265 270 275 gct gct ctg ctc gga ggt ggc tcg gga tct cct ctt ccg ggt gct atc 979 Ala Ala Leu Leu G y G y G y Ser G y Ser Pro Leu Pro G y Ala ile 280 285 290 agc ctg gcg cat cat ggt gtg ttg ttc ctg gat gag gtt agt gag att 1027 Ser Leu Ala His His G y Val Leu Phe Leu Asp u Val Ser u Ile 295 300 305 cca gcg tca atc ctt gat tct ttg agg act cca ttg gaa tac ggc tcg 1075 Pro Ala Ser Ile Leu Asp Ser Leu Arg Thr Pro Leu u Tyr y Ser 310 315 320 325 atc cgc atc atc aga tcc cgc cat gat gtc acc ttc ccc gca cag ttc 1123 Ile Arg Ile Ile Arg Ser Arg His Asp Val Thr Phe Pro Ala n Phe 330 335 340 cag ctc atc ctc gcg gcc aat ccg tgt aga tgc ggt gca gaa cag cct 1171 Sn Leu Ile Leu Ala Ala Asn Pro Cys Arg Cys G y Ala u n Pro 345 350 355 caa gaa tgt gtc tgt tct ggc tca gct cgc gcg acg tac ctt aat aat 1219 n O u Cys Val Cys Ser G y Ser Ala Arg Ala Thr Tyr Leu Asn Asn 360 365 370 ctt tcg ggt ccg ttg agg gat cgc ttg gac atg gtt gtt gcc acc cac 1267 Leu Ser 0 y Pro Leu Arg Asp Arg Leu Asp 'b t Val Val Al a Thr His 375 380 385 tct aaa ggt gca gtg ctg cgt agt gat gac gtt gag gca tct gct ccc 1315 Ser Lys y Ala Val Leu Arg Ser Asp Asp Val u Ala Ser Ala Pro Page 42 IND seqI i st car r ect ed. t xt 390 395 400 405 at t gct gat cgg gt g gca caa gct cgt gag agg gca gct t t c cga t gg 1363 Ilie Ala Asp Ar g Val Ala O31n Ala Ar g O31u Ar g Ala Ala Phe Ar g Tr p 410 415 420 cgc cgt t ct gga ct g gga aat ct t gt t aat gca cac gt a gat cca cac 1411 Ar g Ar g Ser G31y Leu G31y Asn Leu Val Asn Ala Hi s Val Asp Pr o Hi s ri425 430 435 t tc t tg cgg agg aat t tt gcc gca aca gaa gac gcc at g gt g t ac ct c 1459 Phe Leu Ar g Ar g Asn Phe Ala Ala Thr O31u Asp Ala I'b t Val Tyr Leu ri440 445 450 00 ggc gcg t tc ct g gcg gaa gga aca at c t cc caa cgt ggc t gc gat cgg 1507 (3 OyAla Phe Leu Ala (1u 1y Thr Ile Ser 31n Ar g 1y Cys AspAr g 455 460 465 .C gcc at aaaa ct gggt t gg acc tt g tgc gat t t ggat ggg gaa cag cag 1555 A Aa Ile Lys Leu (1y Tr pThr Leu Cys Asp LeuAsp (1y (1u (1n (1n S470 475 480 485 ccc aat ct t gac cat at t gcg cga gcc at g gag ct t cgg ggc act aca 1603 Pr o Asn Leu Asp Hi s Ilie Ala Ar g Ala 'b t O31u Leu Ar g G31y Thr Thr 490 495 500 t ac agt gag gt a gca gca t gat tgact c ccgct tgct g gca 1644 Tyr Ser O31u Val Ala Ala 505 <210> 38 <211> 507 <212> PRT <213> Cor ynebact er i urn gi ut ani cumn <400> 38 I'b t Ala L eu G31y Ar g Th r I Ie Se r Th r Ala O31n L eu G31y Val O31n Ala 1 5 10 Lys li e Val Ar g Val Ou Ala Asn Val G31y Pro G31y Leu Pro G31y Thr 25 Tyr I Ie Val G31y Leu Ala As p Th r Ala Ilie Ser O31u Se r Arg Asp Arg 40 Ilie Lys Thr Ala Val O31n As n Se r G31y Leu I'b t Trp Pro Lys Thr Lys 55 Val Ilie Ilie Asn Leu Ser Pr o Ala Ser I'b t Ar g Lys O31n O31y Se r O31n 70 75 Cys Asp Leu Ala I'b t Thr Val Ala Val Leu Val Ala His O31y Se r Asn 90 Pr o Lys Ala Lys Phe Hi s Ala O31n Asn Thr Leu Phe Leu O31y O31u Val 100 105 110 Ala Leu Asp O31y Thr Leu Leu Pr o Val Thr O31y Val Leu Pro Ala Leu 115 120 125 Leu Aa AaLys (1u (1u (1y Ile (1y Lys Ile Val Ile Pro (1u (1y 130 135 140 Asn Ala O31n O31u Ala O31y Leu Val O31u Asp Pr o Ser Val Phe Leu Ala 145 150 155 160 Hi sSer Ile Asp (1n Val Leu Ar gTr pLeu Asp(1y (1u (1u Aa Leu Page 43 seql i st cor r ect ed. t xt 165 Leu Phe Asn Asp Pr o Mbt Al a Gy 225 Ser Ar g Hi s Leu G u 305 Leu Phe Gy Thr Val 385 G u Al a Hi s Al a Ar g 465 Asp Leu Pr o Asp 195 Al a Ser G n Phe Val 275 Gy Ser Tyr Al a G u 355 Leu Al a Ser Phe Asp 435 Val Cys G u Gy Val Gy I e et 245 Gy Lys SIle SIle Ser 325 Phe Pr o Asn Hi s Pr o 405 Tr p Hi s Leu Ar g G n 485 Gy Hi s Al a 230 I le Pr o Al a Ser Pr o 310 I le G n G n Leu Ser 390 I le Ar g Phe Sy Al a 470 Pr o n Hi s 215 G u G u Val Al a Leu 295 Al a Ar g Leu G u Ser 375 Lys Al a Ar g Leu Al a 455 l le Asn Pr o 200 Met Ar g Al a Ser Leu 280 Al a Ser SIle SIle Cys 360 Sy Sy Asp Ser Ar g 440 Phe Lys Leu 170 G u Asn Ser 185 G u Al a Ar g Leu IVet II e Ile Pro Ser 235 Thr Al a Val 250 Arg Ala Pro 265 Leu G y G y Hi s Hi s G y Ile Leu Asp 315 I e Ar g Ser 330 Leu Al a Al a 345 Val Cys Ser Pro Leu Arg Al a Val Leu 395 Arg Val Ala 410 G y Leu G y 425 Arg Asn Phe Leu Al a G u Leu G y Trp 475 Asp His Ile 490 Val Al a Al a 505 Leu Lys Leu 190 Phe Al a Al a 205 G y Pro Pro 220 Leu Leu Pro Hi s Ser Val Phe Ile Ser 270 G y Ser G y 285 Val Leu Phe 300 Ser Leu Arg Arg His Asp Asn Pro Cys 350 G y Ser Al a 365 Asp Arg Leu 380 Arg Ser Asp G n Al a Ar g Asn Leu Val 430 Al a Al a Thr 445 Sy Thr lle 460 Thr Leu Cys Al a Ar g Al a 175 Pro Asp G u Val G y Ser G u Leu 240 Val G y 255 Pro His Ser Pro Leu Asp Thr Pro 320 Val Thr 335 Arg Cys Arg Ala Asp IVet Asp Val 400 G u Arg 415 Asn Ala Su Asp Ser G n Asp Leu 480 vet u 495 Thr Tyr Ser G u <210> 39 <211> 1173 Page 44 seql i st cor r ect ed. t xt <212> DNA <213> Corynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1150) <223> RXA01458 <400> 39 gccattagag ttcatattta ccaatctaca aggcaactat ttcctattgc tacgatccga acaacgctgt aagcgaacac aggt t gtagg gtggatattc aag Lys gt a Val t at Tyr gac Asp gaa G u ttg Leu ccc Pr o gaa G u gcc Al a gt c Val 150 ctt Leu acc Thr gag G u ct g Leu at t SIle gct Al a gag G u aac Asn cag G n gag G u aac Asn 120 at g Met acc Thr agc Ser gt g Val aac Asn 200 tgg Tr p cat Hi s ggc Gy gtt Val aac Asn at t SIle gtt Val acc Thr t ac Tyr agc Ser gac Asp 170 tcg Ser gcg Al a ggt Gy at t SIle cca Pr o gt g Val at g Met 75 t cc Ser tt c Phe gcg Al a ct c Leu gcc Al a 155 ggc Gy aaa Lys gaa G u gct Al a ccg Pr o ggt Gy t cc Ser acc Thr cga Ar g cct Pr o ttc Phe aac Asn 140 gca Al a acc Thr gct Al a tct Ser at c l Ie gga Gy cct Pr o 45 at c SIle act Thr tgg Tr p ccc Pr o at c SIle 125 at t SIle acc Thr gca Al a ccc Pr o caa G n 205 cct Pr o aca Thr acg Thr agt Ser gt g Val ct g Leu ggc G y 110 tct Ser ccc Pr o gga Gy at c l Ie ggc Gy 190 gca Al a gt g Val at c SIle aat Asn gct Al a gtt Val 80 acc Thr t cc Ser gag G u gaa G u ttc Phe 160 act Thr gag G u at c l Ie gtg aat cgc cga atc Val Asn Arg Arg Ile 1 ctg ctg gca tcg ttg Leu Leu Ala Ser Leu aac ttg agc gtg cct Asn Leu Ser Val Pro acg ctt ggt cag gtc Thr Leu Gy G n Val gat ctg gat gag acc Asp Leu Asp G u Thr cgt tcc ggc atg aca Arg Ser G y IVet Thr gat gac aca atc gtt Asp Asp Thr lie Val 100 acc gag gaa gtc gaa Thr u u Val u 115 tct tcc gca acg atc Ser Ser Ala Thr lle 130 gtt gaa gtt gca gaa Val G u Val Ala G u 145 gaa ccc ggc gac aaa G u Pro y Asp Lys 165 ccc ggc gat gca caa Pro G y Asp Ala G n 180 atc acg att tcc tac Ile Thr Ile Ser Tyr 195 act ttg agg gaa cac Thr Leu Arg G u His 210 tca atg ttg tcg gtg ccg gat gat tct tcg gtg gcg ttg ttg ggt att Page Pro Asp 215 Asp Ser Ser Val Ala Le 220 seql i st cor r ect ed. t xt u Leu G y I e Ser IVet Leu Ser Val cct tcg agc gcg att gag gtt gat tac Pro Ser Ser Ala lie G u Val Asp Tyr 230 235 ccg agc gct ggc atg atg ttt tcg ttg Pro Ser Ala Gy Met Mbt Phe Ser Leu 250 cct ggc gcg ctg aat ggt ggc aag ttt Pro y Ala Leu Asn Gy y Lys Phe 265 270 gcg gag gac ggg tcg gtg ggc ccg att Ala u Asp y Ser Val y Pro Ile 280 285 cgc gct gcg gag gac gcg ggc gcg gaa Arg Ala Ala u Asp Ala y Ala u 295 300 aat tgc gcg gag gcg atg agt gcg aag Asn Cys Ala G u Ala IVet Ser Ala Lys 310 315 aag gtg gat tcg ttg tct cag gca atc Lys Val Asp Ser Leu Ser G n Ala lie 330 gag ggc tct gat ttc cag acg tgt ggc G u G y Ser Asp Phe G n Thr Cys G y 345 350 ttc <210> <211> 350 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> Val Asn Arg Arg lie Lys Thr Leu Thr 1 5 Leu Leu Ala Ser Leu Val Ser Ile Asp Asn Leu Ser Val Pro Tyr Ala Ala G u 40 Thr Leu y n Val Asp y u Asp 55 Asp Leu Asp G u Thr G u G y Asn Leu 70 Arg Ser y IVet Thr Leu Ser G n Val Asp Asp Thr Ile Val Pro Ile u n 100 105 Thr u u Val u u Ser Asn Arg 115 120 aac Asn gcg Al a 255 gt c Val ggc G y gt g Val cct Pr o gat Asp 335 ttg Leu 240 gt c Val gct Al a ggt G y ttt Phe cag G n 320 cag G n gat Asp gat Asp act Thr gcg Al a 290 agc Ser at g Met gct Al a ggt G y ctt Leu 260 acc Thr aag Lys gcg Al a at c SIe t ac Tyr 340 835 883 931 979 1027 1075 1123 1170 1173 tagtttttag acgctgaggt Trp G y Al a 10 His Ile Pro G y Pro G y Val Val Ser Asn IVet Thr I e Ser Ar g 90 Val Phe Pro Thr Al a Phe Page 46 l Ie Sy Pr o I le Thr Tr p Pr o SIle 125 Leu Asn SIe Ser Ser Phe G n Ser seql i st cor r ect ed. t xt Ser Ser Ala Thr lie Ala Ala IVet Asn Tyr Leu Asn II e Pro Val u 130 Val 145 G u Pr o SIle Thr Ser 225 G u Val Gy I e Leu 305 Asp Met Val Gy Asp SIle 195 Ar g Leu SIle Lys Gy 275 Hi s Pr o Thr Al a G u Lys 165 G n Tyr Hi s Val Gy 245 Ser SIle Val Asp Leu 325 Asn 135 Leu Leu SIle Ar g Asp 215 Ser Ser Gy Gu Al a 295 Cys Val Gy Thr Ser Val Asn 200 Asp Ser Al a Al a Asp 280 Al a Al a Asp Ser Asp SIle Ar g 185 Asp Ser Al a Gy Leu 265 Gy G u G u Ser Asp 345 Ser Asp 170 Ser Al a Ser l Ie Met 250 Asn Ser Asp Al a Leu 330 Phe Al a 155 Gy Lys G u Val G u 235 Met Sy Val Al a bet 315 Ser G n 140 Al a Thr Al a Ser Al a 220 Val Phe Sy Gy Sy 300 Ser G n Thr Thr Al a Pr o G n 205 Leu Asp Ser Lys Pr o 285 Al a Al a Al a Cys SIle Ser 175 Asp Thr Sy Asn Al a 255 Val Gy Val Pr o Asp 335 Phe 160 Thr Su I e I e Leu 240 Val Al a Gy Phe G n 320 G n <210> 41 <211> 1119 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1096) <223> RXA01654 <400> 41 cttttgctgt tatgaaagtg aactaaagat atctatgcgc agaccggtct cacgccgcgc catttttgca acatctgttt tggttgcggg ggtgagcatc atg tca cct tcg gcc Vet Ser Pro Ser Ala 1 aac gca gct gag gct ccg gca tcg gaa tgg gtg aat acg aca gcg atc Asn Ala Ala u Ala Pro Ala Ser u Trp Val Asn Thr Thr Ala lie 15 gta gat caa gcg aat gct cag ttg tcg cag ttt ggc gtg agt ctt gac Val Asp n Ala Asn Al a G n Leu Ser G n Phe y Val Ser Leu Asp 30 Page 47 cga Ar g gca Al a gt a Val ct g Leu gca Al a gcg Al a t cc Ser tgc Cys 150 at c SIle gac Asp aac Asn aat Asn aat Asn 230 ggt Sy gcg Al a gtt Val agt gca gca gaa ctt ttt Ser Ala Ala G u Leu Phe ctt Leu gag G u aac Asn ct a Leu gca Al a 120 t at Tyr ctt Leu gca Al a ttc Phe gac Asp 200 t ac Tyr caa G n gt g Val cgt Ar g ctt Leu 280 ccg Pr o agt Ser gaa G u gag G u cct Pr o ccc Pr o gt g Val cac Hi s 170 gt c Val t cc Ser ggt Sy gt a Val act Thr 250 gat Asp tct Ser gcc Al a cag G n cgt Ar g ct g Leu gaa G u acc Thr 140 gga Gy gga Gy gat Asp at c SIle acc Thr 220 aaa Lys gat Asp ggt gty ggt Gy S( gat Asp gat Asp gtt Val acc Thr cac Hi s t cc Ser 125 ccg Pr o act Thr aaa Lys acc Thr gag G u 205 gcg Al a aac Asn gag G u gct Al a gt a Val 285 aag Lys gt g Val gat Asp cga Ar g 110 agg Ar g at c SIle gat Asp tcg Ser gga Gy 190 ttc Phe aat Asn gga Gy cgc Ar g ccg Pr o 270 at t SIle eql i st cor r ect ed. t xt gat cag gca aac tc Asp G n Ala Asn Se c caa att r Gn lie gtt Val gag G u ct g Leu 95 gtt Val at t SIle t at Tyr gca Al a ggc Gy 175 aca Thr ggt Gy gct Al a gtt Val at g let 255 ct g Leu cct Pr o cca Pr o cag G n 80 caa G n cct Pr o gt a Val ct c Leu gat Asp 160 gat Asp gt g Val t cc Ser gt c Val gct Al a 240 acg Thr at t SIle gat Asp cca Pr o acc Thr gaa G u t cc Ser gga Gy gag G u aac Asn 145 ggg Gy gca Al a gt g Val aat Asn ggc Sy 225 act Thr at g Met gca Al a t ac Tyr tct Ser gtt Val cag G n t ca Ser gaa G u 130 gga Gy cgc Ar g gt c Val t ac Tyr gca Al a 210 ggt Sy ggc G y tct Ser gat Asp aac Asn 290 ggc Gy caa G n gt g Val tgg Tr p 115 cag G n aat Asn aag Lys cgt Ar g aac Asn 195 gag G u ggc Sy t ac Tyr cag G n ggt G y 275 ct g Leu ggc Gy aag Lys at g Met 100 ttt Phe ggt G y t cc Ser at c SIle tcg Ser 180 gcg Al a ttg Leu gt g Val acc Thr gt g Val 260 cgt Ar g gca Al a gat Asp cag G n gca Al a ggt G y gat Asp aaa Lys at g Met ggt Sy 165 gct Al a cct Pr o agc Ser act Thr tgt Cys 245 tgt Cys gt g Val tgc Cys 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 gcc act ccg ttg cag gga cct ttc ttc atg Ala Thr Pro Leu Gn y Pro Phe Phe Iet acg ctg tca gtg aac Thr Leu Ser Val Asn 305 Page 48 seqI i st car r ect ed. t xt at g gat act gt c ct a act gat ttg gat tcg cag gat ct t ccc ggt cga I'b t Asp Thr Val Leu Thr Asp Leu Asp Ser O31n Asp Leu Pro O31y Ar g 310 315 320 325 ggt ttt cag cca act gct gga tagaatttag aaaat ccgcc gtt G31y Ph e (31n Pro Thr Ala G31y 330 <210> 42 <211> 332 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn 1 075 1119 <400> 42 I'bt Ser Asn Thr 03 y Val Asn Ser Thr Ser 03 u Val Ser 03 n 03 y Ser O31u (1u 130 As n (1y 145 O31y Ar g Ala Val Val Ty r As n A a 210 O31y (1y 225 Thr 03 y I'b t Ser Pr o Ser Thr A a Ser Leu (31n I Ie (31y O31y (3 n Lys Val rvb t 1 00 Tr p Phe 115 (31n O31y Asn Ser Lys I I e Ar g Ser 1 80 As n A a 1 95 (3 u Leu (3 y Val Ty r Thr (3 n Val 260 A a le As p As p O31n A a O31y As p Lys vb t O31y 1 65 A a Pr a Ser Thr cys 245 Cys As n A a A a 03 u ValI Ar g A a ValI 70 Leu A a A a Ser Cy s 150 li1e As p As n As n As n 230 O31y A a As p Ser A a 55 ValI Pr a Thr Pr a Le u 135 Thr Thr Ser A a Thr 215 O31y Le u O31y O31n A a 40 Le u (31u As n Le u A a 120 Tyr Le u A a Ph e As p 200 Tyr O31n ValI Ar g A a A a Ser O31n Tyr O31y 1 ValI O31y A a O31y Tr p 1 Tyr As n O31u Tr p O31y 265 A a 10 As n O31u Pr a Ser O31u 90 O31u Pr a Pr a ValI Hi s 1 70 ValI Ser O31y ValI Thr 250 As p Pro Ala Se r O31u Tr p Val A a Le u Tyr Le u 75 li1e ValI O31u O31y Thr 1 55 Cys O31y ValI ValI Cys 235 A a Ser O31n Ph e A a O31n Ar g Le u O31u Thr 140 O31y O31y As p I Ie Thr 220 Ly s As p O31y Le u As p As p ValI Thr Hi s Ser 125 Pr a Thr Ly s Thr O31u 205 A a As n O31u A a O31n O31n ValI O31u Le u ValI I Ie Tyr A a O31y 1 Thr O31y A a ValI vb t 255 Le u Ph e A a Pr a O31n O31n Pr a ValI Le u As p 1 As p ValI Ser ValI A a 240 Th r I Ie A a Asp (31 y 275 Arg Val Val cOy Leu Val Ser cOy Oy Val Ilie Pro Asp 280 285 Page 49 seqi i st car r ect ed. t xt Ty r Asn Leu Ala Cys Ala Thr Pro Leu On Gy Pro Phe Phe l'bt Pro 290 295 300 Thr Leu Ser Val Asn l'bt Asp Thr Val Leu Thr Asp Leu Asp Ser 03 n 305 310 315 320 Asp Leu Pro G31y Arg G31y Phe O31n Pro Th r Ala O31y 325 330 <210O> 43 <21 1> 2049 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (2026) <223> RXN01868 <400> 43 t gacaggct a cct t ct gggg t ggacat gat ccccaacgct ccacaaaccc t gt ggcggt a aat cccct ag agt aggccac caacccactt gtggcaccaa cgc Ar g cgc Ar g g ac As p t at Tyr ccg Pr o tt c Ph e gt a ValI t at Tyr gag O31u cgc Ar g 1 50 gt c ValI gt g ValI cat Hi s t ca Ser g at As p gct A a tt c Ph e at c I Ie 120 gca A a ct c Le u ggc G31y ggc G31y at c I Ie at g vb t g at As p ct c Le u gt g ValI t cc Ser gcc A a t ac Tyr ct g Le u ac g Thr gt c ValI g at As p ct g Le u 75 ggc G31y gag O31u G31y gaa O31u t t g Le u 155 t gg Tr p tt c Ph e aag Ly s act Thr aac As n g aa O31u aag Ly s ct c Le u act Thr 140 g at As p ct t Le u c ac Hi s g aa O31u 45 g ac As p ag g Ar g ct g Le u g at As p ggc G31y 125 ct c Le u aac As n g ac As p aag Ly s g ac As p gcc A a ct g Le u g ac As p t ct Ser 110 ct g Le u gcg A a ag c Ser ac c Thr 15 ct g Le u act Thr at c I Ie t ct Ser cgt Ar g 95 t cc Ser ccc Pr o gcc A a cgc Ar g c ac Hi s cgc Ar g gga G31y aac As n gt t ValI 80 g aa O31u t cc Ser g at As p t ac Tyr ct c Le u 1 60 at g vb t 1 at c I Ie g at As p cgc Ar g gct A a gct A a ggc G31y aac As n gag O31u aaa Ly s 145 tt c Ph e aag gat ctt Lys Asp Leu at t I Ie g at As p gca A a gct A a aac As n gt t ValI g aa O31u gct A a 130 gag O31u ggt G31y g ac As p g aa O31u ac a Thr gt t ValI t cg Ser gcg A a 1 00 gt c ValI t ac Tyr gt t ValI t cg Ser t at Tyr g at As p g aa O31u ct t Le u gca A a tt t Ph e cca Pr o gcc A a cgc Ar g gag (31u gct A a 1 Page gct t cc gct gcc Ala Ser Ala Ala ggc Gy ccc Pr o t cc Ser ct c Leu 230 cag G n acc Thr t cc Ser gca Al a cat Hi s 310 ct g Leu ccc Pr o aaa Lys ggc Gy gac Asp 390 gt c Val tgg Tr p gaa G u 200 ggc Gy cac Hi s tgg Tr p gaa Gu gcc Al a 280 cgc Ar g cct Pr o gcc Al a acc Thr ggc Gy 360 gac Asp gag G u acc Thr gca Al a 170 gt c Val ggc Gy ccg Pr o aac Asn acc Thr 250 agc Ser gag G u gt g Val agc Ser t ac Tyr 330 gag G u ccc Pr o gt g Val ggc Gy caa G n 410 gt g Val gcg Al a gac Asp ggc Gy 235 tgg Tr p caa G n caa G n ggc Gy t cc Ser 315 cgt Ar g cgt Ar g gac Asp gac Asp aag Lys 395 acc Thr seql i st cor r ect ed. t cga att gtc gcc ctg gaa acg Arg Ile Val Ala Leu u Thr gaa atc gct gct Su Ile Ala Ala aag Lys ct g Leu cag G n 220 ttg Leu cac Hi s gca Al a aaa Lys gag G u 300 aag Lys gat Asp gcc Al a aag Lys aac Asn 380 at c SIle gt c Val acc Thr cca Pr o 205 cgc Ar g ctt Leu at c SIle aac Asn gat Asp 285 gaa G u gag G u cgc Ar g ct g Leu tgg Tr p 365 t cc Ser ggc G y aac Asn cgc Ar g 190 cca Pr o ct g Leu gt c Val ttg Leu ttc Phe 270 cga Ar g at c SIle cac Hi s at t SIle gaa G u 350 cgc Ar g cgc Ar g aaa Lys gcc Al a ct g Leu 430 gac gcc Asp Al a aag gtc Lys Val gta t cg Val Ser gac gac Asp Asp 240 agg tct Arg Ser 255 gac ttc Asp Phe tgg aag Trp Lys ggg caa G y G n atg ctt IVet Leu 320 t cc aac Ser Asn 335 aag ttg Lys Leu t cc tac Ser Tyr aag ggc Lys G y cca gcc Pro Al a 400 ttc tac Phe Tyr 415 cgc gca Arg Ala Page 51 gt a Val cgc Ar g at g Met 225 cgc Ar g cga Ar g t at Tyr cgt Ar g cga Ar g 305 gag G u ct c Leu ggc Sy gaa G u t cc Ser 385 gac Asp aac Asn cca Pr o gcc Al a acg Thr 210 at g Met ct c Leu gca Al a ggc Gy gct Al a 290 ttc Phe ct c Leu gaa G u aaa Lys ggc G y 370 gcg Al a cgc Ar g ccc Pr o ttc Phe 180 t ac Tyr ct c Leu t ca Ser gat Asp ct g Leu 260 aaa Lys ggc Sy gaa G u gac Asp at g Met 340 aac Asn gaa G u ct c Leu gaa G u gt c Val 420 gac Asp 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 gac atc acc Asp II e Thr ccc gca gcc atc Pro Ala Ala lle gaa gca gaa gcc gca gaa Gu Ala Gu Ala Ala G u 440 seql i st cor r ect ed. t xt aac ttc ggt gca atc ggt gct gtg atc gga Asn Phe Gy Ala I Ie y Ala Val I le y cac gaa atc ggc Hi s u I I e G y 455 gac ggc aac ctc Asp G y Asn Leu 470 gag cag ctc acc G u G n Leu Thr gcc gtc ctg acc Ala Val Leu Thr 505 ttc act ctc ggc Phe Thr Leu G y 520 gtc gtt gcc tac Val Val Al a Tyr 535 acc tca cca gtc Thr Ser Pro Val 550 gcc gag caa gaa Ala a u G n u cgc gtg tgg cgc Arg Val Trp Arg 585 gcc atc gac cca Ala lie Asp Pro 600 gga aac gtc gct Sy Asn Val Al a 615 cct gtg tac atc Pro Val Tyr Ile 630 ttggtattga aaa <210> 44 <211> 642 <212> PRT <213> Corynebact <400> 44 IVet Lys Asp Leu 1 Ile Ile Pro Asp Asp Asp Al a u cac Hi s aac Asn t ca Ser 490 tct Ser gaa G u gaa Gu caa G n ttc Phe 570 acc Thr cac Hi s gaa G u ggc Gy t cc Ser 475 cgt Ar g gaa G u aac Asn aag Lys aaa Lys 555 aac Asn aaa Lys t cc Ser tt c Phe ttt Phe 460 tgg Tr p ct g Leu gga G y at c SIle t ac Tyr 540 ttc Phe ggt Sy at c SIle cct Pr o t ac Tyr 620 445 gac Asp tgg Tr p gt c Val at c SIle ggt 525 ct c Leu gaa G u ct c Leu cgc Ar g gca Al a 605 gaa G u gat Asp acc Thr acc Thr gac Asp 510 gac Asp gca Al a gca Al a caa G n cca Pr o 590 gaa G u gca Al a caa G n gac Asp caa G n 495 acc Thr ct c Leu gac Asp gaa G u cgc Ar g 575 cag G n ttc Phe ttc Phe ggc Gy gaa G u 480 ttc Phe gac Asp ggc G y cgt Ar g ggc Sy 560 ct c Leu at g Met cgc Ar g gac Asp 450 caa t ac G n Tyr cgc t cc Arg Ser gga ct c G y Leu gtc aac Val Asn 515 ttg ggc Leu G y 530 caa acc G n Thr gag gaa G u G u ctg t cc Leu Ser gtc caa Val G n 595 aat gt c Asn Val 610 ccc gaa Pro G u gac Asp gca Al a gt c Val 500 ggt G y at c SIle ttt Phe ggc Sy tgg Tr p 580 t ac Tyr at c SIle gat Asp ggc Gy ttc Phe 485 cct Pr o gaa G u gct Al a gaa Gu ct g Leu 565 gct Al a ct g Leu gcc Al a gca Al a 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2036 2049 aag cca gaa gag cgc cta gct atc tgg Lys Pro u u Arg Leu Ala lie Trp 635 640 t agt t gt t ag er i um gl ut am cum Tyr Arg Phe Val Asn y Leu Trp Leu Asp Thr His 5 10 Asp Arg Ala Val Asp y Thr Phe His Lys Leu Arg 25 Gu Asp Val His Gu lie Val Lys Gu Asp Thr Gy Page 52 seql i st cor r ect ed. t xt Phe IVet Asp Thr Asp Ala lie Asn Ar g Al a Al a Gy Asn G u Lys 145 Phe Thr Val Ar g Met 225 Ar g Ar g Tyr Ar g Ar g 305 G u Leu Gy G u Gy Gy Ser Gy Ser 115 Tyr Hi s Leu SIle Thr 195 Leu Pr o Pr o Gy Thr 275 Val Val Val Tr p Phe 355 Leu Leu Al a Phe Pr o Al a Ar g G u Al a 165 Al a Asn Ser Tyr Tr p 245 Leu Leu Leu Ar g Tyr 325 Thr Al a Phe Tyr Pr o 70 Phe Val Tyr G u Ar g 150 Al a Gy Pr o Ser Leu 230 G n Thr Ser Al a Hi s 310 Leu Pr o Lys Gy Al a Ser Leu Asp Al a Al a G y Phe Val lile 120 G u Al a 135 IVet Leu Ser Al a His Tr p Thr G u 200 Al a G y 215 Asp Hi s Leu Trp G u G u G y Al a 280 G u Arg 295 Phe Pro Val Ala Al a Thr I e G y 360 Ser Asp 375 Al a Al a Tr p 105 G n Hi s Gy Al a Asp 185 Leu Leu Leu Al a SIle 265 Thr b t Al a Al a Ar g 345 Tyr Leu Asp Leu 90 Val Ser Al a Tyr Al a 170 Val Gy Pr o Asn Thr 250 Ser G u Val Ser Tyr 330 G u Pr o Val Leu Gy G u Gy G u Leu 155 Ar g Val Al a Asp Gy 235 Tr p G n G n Gy Ser 315 Ar g Ar g Asp Asp Asn G u Lys Leu Thr 140 Asp SIle Lys Leu G n 220 Leu Hi s Al a Lys G u 300 Lys Asp Al a Lys Asn 380 Ar g Leu Asp Sy 125 Leu Asn Val Thr Pr o 205 Ar g Leu SIle Asn Asp 285 G u G u Ar g Leu Tr p 365 Ser Leu Asp Ser 110 Leu Al a Ser Al a Ar g 190 Pr o Leu Val Leu Phe 270 Ar g SIle Hi s SIle G u 350 Ar g Ar g Ser Ar g Ser Pr o Al a Ar g Leu 175 Asp Lys Val Asp Ar g 255 Asp Tr p Sy vbt Ser 335 Lys Ser Lys Ser Ala Phe Leu Hi s 385 Asp Tyr u Leu y Lys Ile y Lys Pro Ala 390 395 400 Page 53 seqi i st car r ect ed. t xt Val Thr Thr Pro On Thr Val Asn Asp Arg Asp Ou Trp 405 Al a Phe Tyr 415 As n Pr o O31y Ser 465 As p Ser O31y O31y O31y 545 Al a Ph e Al a cys ValI 625 li1e ValI Ph e 435 ValI Tyr Ser Le u As n 515 O31y Thr O31u Ser O31n 595 ValI O31u As n Pr o O31y O31y Ph e 485 Pr o O31u Al a O31u Le u 565 Al a Le u Al a Al a As p O31u Hi s As p 470 O31u Al a Ph e ValI Thr 550 Al a Ar g Al a O31y Pr o 630 I Ie Al a O31u 455 O31y O31n ValI Thr ValI 535 Ser (31u ValI I Ie As n 615 ValI Thr (31u 440 li1e As n Le u Le u Le u 520 Al a Pr a O31n Tr p As p 600 ValI Tyr Ph e 425 Al a O31y Le u Thr Thr 505 O31y Tyr ValI O31u Ar g 585 Pr a Al a I Ie 410 Pr a Al a Hi s As n Ser 490 Ser O31u O31u O31n Ph e 570 Thr Hi s O31u Ly s Al a O31u O31y Ser 475 Ar g O31u As n Ly s Ly s 555 As n Ly s Ser Ph e Pr a 635 I Ie Ph e 445 As p Tr p ValI I Ie O31y 525 Le u O31u Le u Ar g Al a 605 O31u O31u Le u 430 O31y As p Thr Thr As p 510 As p Al a Al a O31n Pr a 590 O31u Al a Ar g Ar g Al a O31n As p O31n 495 Thr Le u As p O31u Ar g 575 O31n Ph e Ph e Le u <210O> <21 1> 1734 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1711) <223> FRXA01868 <400> at gcggat ct gaacaggct g t ct gt t gct a act cat cgt t t t cgcagct g ct ct cggcga act ggaccgt gaaggcgt t g gcgcgccagt aggt t t ct gg gt g gag aag gat t ct 115 Val 03 u Lys Asp Ser 1 t cc t cc aac gaa t cc gt c gcc t at gt c at c cag t cc ggc ctc ggc ctg 163 Ser Ser Asn Ou Ser Val Ala Tyr Val lieOn Ser O31y Leu O31y Leu Page 54 seql i st cor r ect ed. t xt ccc Pr o gcc Al a cgc Ar g ct g Leu gac Asp aag Lys gt a Val gac Asp agg Ar g 150 gac Asp tgg Tr p ggg Gy at g Met t cc Ser 230 aag Lys t cc Ser gag G u aaa Lys ttc Phe acg Thr gt a Val cgc Ar g at g lbt 120 cgc Ar g cga Ar g t at Tyr cgt Ar g cga Ar g 200 gag G u ct c Leu ggc Gy gaa G u t at Tyr cac Hi s ct g Leu at c SIle acc Thr ct g Leu ccg Pr o ccc Pr o gga Gy acc Thr 170 gt c Val gt c Val gt c Val tgg Tr p ttc Phe 250 ct c Leu t ac Tyr gtt Val tcg Ser gct Al a 75 tac Tyr ct c Leu t ca Ser gat Asp ct g Leu 155 aaa Lys ggc Gy gaa G u gac Asp at g Mbt 235 aac Asn gaa G u cgc Ar g gag G u gct Al a gct Al a aac Asn agt Ser t ac Tyr tgg Tr p 140 ttg Leu ct g Leu ct g Leu agg Ar g t ac Tyr 220 acg Thr gcg Al a ttc Phe gag G u cgc Ar g 45 gct Al a ggc Gy ccc Pr o t cc Ser ct c Leu 125 cag G n acc Thr t cc Ser gca Al a cat Hi s 205 ct g Leu ccc Pr o aaa Lys ggc Gy gag G u at g let t cc Ser cac Hi s acc Thr gca Al a 110 gac Asp ct a Leu gag G u ggc Gy gag G u 190 ttt Phe gtt Val gcc Al a at c SIle t cc Ser 270 gca Al a ct c Leu gct Al a tgg Tr p gaa G u 95 ggc Gy cac Hi s tgg Tr p gaa G u gcc Al a 175 cgc Ar g cct Pr o gcc Al a acc Thr ggc Gy 255 gac Asp cac Hi s ggc Gy gcc Al a gat Asp ct c Leu ct c Leu ct c Leu gca Al a at t SIle 160 acc Thr at g Met gca Al a gcc Al a cgc Ar g 240 t ac Tyr ct g Leu gcc Al a t ac Tyr gca Al a gt c Val ggc Gy ccg Pr o aac Asn acc Thr 145 agc Ser gag G u gt g Val agc Ser t ac Tyr 225 gag G u ccc Pr o gt g Val gaa G u ttg Leu cga Ar g gt g Val gcg Al a gac Asp ggc Gy 130 tgg Tr p caa G n caa G n ggc Gy t cc Ser 210 cgt Ar g cgt Ar g gac Asp gac Asp act Thr gat Asp at t SIle aag Lys ct g Leu cag G n 115 ttg Leu cac Hi s gca Al a aaa Lys gag G u 195 aag Lys gat Asp gcc Al a aag Lys aac Asn 275 ct c Leu aac Asn gtc Val acc Thr cca Pr o 100 cgc Ar g ctt Leu at c SIe aac Asn gat Asp 180 gaa G u gag G u cgc Ar g ctg Leu tgg Tr p 260 t cc Ser aag ggc tcc gcg ttc ctc cat gac tat gag Lys G y Ser Al a Phe Leu Hi s Asp Tyr G u ctg ggc aag atc ggc aaa Leu y Lys Ile G y Lys Page seql i st cor r ect ed. t xt 280 290 acc gtc aac gcc Thr Val Asn Ala cca gcc Pro Al a 295 gac cgc gac gaa Asp Arg Asp G u tgg Tr p 300 gtc acc acc cca Val Thr Thr Pro caa G n 305 ttc Phe 310 tac aac ccc gt g Tyr Asn Pro Val aac gac atc acc Asn Asp Ile Thr ttc Phe 320 ccc gca gcc at c Pro Ala Ala lle cgc gca cca ttc Arg Ala Pro Phe ttc Phe 330 gac ccc gaa gca Asp Pro G u Ala gaa G u 335 gcc gca gaa aac Ala Ala G u Asn ttc ggt Phe G y 340 gca at c ggt Al a l e G y caa ggc agc Sn G y Ser 360 gtg atc gga cac Val IIe G y Hi s gaa G u 350 at c ggc cac I e G y Hi s caa tac gac ggc G n Tyr Asp G y gac ggc aac ctc Asp G y Asn Leu 365 gag cag ctc acc G u 0 n Leu Thr aac Asn tca Ser 385 ggc ttt gac gat G y Phe Asp Asp 355 tcc tgg tgg acc Ser Trp Trp Thr 370 cgt ctg gtc acc Arg Leu Val Thr gac gaa Asp u 375 gac cgc tcc gca Asp Arg Ser Ala ttc Phe 380 caa G n 390 ttc agc gga ctc Phe Ser G y Leu cct gcc gtc ctg Pro Al a Val Leu acc Thr 400 tct gaa gga atc Ser G u G y Ile acc gac ggc gtc Thr Asp y Val aac Asn 410 ggt gaa ttc act G y G u Phe Thr ggc gaa aac at c Sy G u Asn Ile ggt gac Gy Asp 420 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1734 ctc ggc gga Leu G y G y gac cgt ggc Asp Arg G y 440 ggc atc gct gtc G y I e Al a Val gtt Val 430 gcc tac gaa aag Ala Tyr G u Lys tac ctc gca Tyr Leu Ala 435 ttc gaa gca Phe G u Ala caa acc ttt gaa G n Thr Phe G u tca cca gtc caa Ser Pro Val G n aaa Lys 450 gaa ggc G 4u G 455 gcc gag gaa ggc Ala G u u G y ctg Leu 460 gcc gag caa gaa Ala u G n G u aac ggt ctc caa Asn y Leu G n cgc Ar g 470 ctc ttc ctg tcc Leu Phe Leu Ser gct cgc gtg tgg Ala Arg Val Trp cgc Ar g 480 acc aaa atc cgc Thr Lys lie Arg cag atg gcc gtc 0 n Mat Ala Val caa G n 490 tac ctg gcc atc Tyr Leu Ala lle cca cac tcc cct Pro His Ser Pro gca gaa Al a u 500 ttc cgc tgc Phe Arg Cys ttc gac gtc Phe Asp Val 520 gtc atc gcc gga Val Ile Ala y aac Asn 510 gtc gct gaa ttc Val Ala G u Phe tac gaa gca Tyr G u Ala 515 gaa gag cgc G u G u Arg ccc gaa gat gca Pro G u Asp Ala gtg tac atc aag Val Tyr Ile Lys cca Pr o 530 cta gct atc tgg Leu Ala lie Trp 535 tagt t gt t ag ttggtat t ga aaa <210> 46 Page 56 seqI i st car r ect ed. t xt <21 1> 537 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 46 Val G31u Lys Asp Ser Ser Al a Ty r Al a ValI O31y Pr o As n Th r 145 Ser O31u ValI Ser Ty r 225 O31u Pr o ValI O31y O31n 305 Le u Th r As p I Ie Ly s Le u O31n 115 Le u Hi s Al a Ly s O31u 1 95 Ly s As p Al a Ly s As n 275 li1e ValI Le u Al a Ser Al a Ar g Pr o Le u ValI Le u Ph e 1 65 Ar g I Ie Hi s I Ie O31u 245 Ar g Ar g Ly s Al a Le u 325 Ser Ser Pr o As p Al a Ty r Arg Leu Leu 03 u 70 As p Al a Lys Val Val Ser As p As p 135 Ar g Ser 150 As p Phe Tr p Lys G31y (1n I'b t Leu 215 Ser As n 230 Lys Leu Ser Tyr Lys 03 y Pr o Al a 295 Phe Tyr 310 (31u Al a O31u O31u Al a Thr 1 05 vb t Le u Al a G31y Al a 1 85 Ph e Le u O31u Ly s G31y 265 Al a Ar g Pr o ValI Tyr ValI Ser Al a 75 Tyr Le u Ser As p Le u 1 55 Ly s G31y O31u As p vb t 235 As n O31u Le u O31u ValI 315 Al a Ar g O31u Al a Al a As n Ser Tyr Tr p 140 Le u Le u Le u Ar g Tyr 220 Thr Al a Ph e Hi s Tr p 300 As n Tyr O31u Ar g Al a G31y Pr a Ser Le u 125 O31n Thr Ser Al a Hi s 205 Le u Pr a Ly s G31y As p 285 ValI As p ValI (31u vb t Ser Hi s Thr Al a 110 As p Le u (31u G31y (31u 1 Ph e ValI Al a I Ie Ser 270 Tyr Thr I Ie lIe Al a Le u Al a Tr p (31u G31y Hi s Tr p (31u Al a 1 Ar g Pr a Al a Thr G31y 255 As p (31u Thr Thr (31n Hi s G31y Al a As p Le u Le u Le u Al a I Ie 1 Thr vb t Al a Al a Ar g 240 Tyr Le u Le u Pr a Ph e 320 Pro Ala Ala Ilie Arg Ala Pro Phe Phe Asp Pro Ou Ala Ou Ala Page 57 seql i st cor r ect ed. t xt Al a u Asn Phe y Al a I e y Ala Val I le y Hi s Gu lie y 350 340 Hi s Asn Ser 385 Ser G u G u G n Phe 465 Thr Hi s G u Lys Phe 355 Tr p Leu Gy SIle Tyr 435 Phe Gy SIle Pr o Tyr 515 G u Asp Thr Thr Asp 405 Asp Al a Al a G n Pr o 485 G u Al a Ar g Gy G u 375 Phe Asp Gy Ar g Gy 455 Leu Met Ar g Asp Al a 535 Ser 360 Asp Ser Gy Gy Gy 440 Al a Phe Al a Cys Val 520 I le 345 G n Ar g Gy Val Leu 425 G n G u Leu Val Asn 505 Pr o Tr p Asp Al a Val 395 Gy SIle Phe Gy Tr p 475 Tyr SIle Asp Gy Phe 380 Pr o G u Al a G u Leu 460 Al a Leu Al a Al a Asp 365 G u Al a Phe Val Thr 445 Al a Ar g Al a Gy Pr o 525 Asn Leu Leu Leu 415 Al a Pr o G n Tr p Asp 495 Val Tyr Leu Thr Thr 400 Gy Tyr Val G u Ar g 480 Pr o Al a SIe <210> 47 <211> 426 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(403) <223> FRXA01869 <400> 47 tgacaggcta cct t ctgggg tggacatgat ccccaacgct ccacaaaccc tgtggcggta aatcccctag agtaggccac cgc ttt gtc Arg Phe Val cgc gcg gtg Ar g Al a Val gac gt c cat Asp Val Hi s aat ggc ctg tgg ctt gac Asn Gy Leu Trp Leu Asp gac ggc acg ttc cac aag Asp Gy Thr Phe His Lys 30 gag atc gtc aag gaa gac G u lie Val Lys G u Asp 45 acc cac Thr His 15 ctg cgc Leu Arg act gga Thr G y Page 58 caacccactt gtggcaccaa atg aag gat ctt tat IVet Lys Asp Leu Tyr 1 atc att ccc gac gat Ile Ile Pro Asp Asp gat gat gct gaa gaa Asp Asp Al a u G u cgc gca ggc aca ctt Arg Ala Gy Thr Leu tat gcc tca ttt atg gat a Tyr Ala Ser Phe IVet Asp T ccg ctc gat gcg gat ctg a Pro Leu Asp Ala Asp Leu A 75 tcg cag ctg ctc tcg gcg a Ser G n Leu Leu Ser Al a A taggtttctg ggtggagaag gat <210> 48 <211> 101 <212> PRT <213> Corynebacteri um gl u <400> 48 IVet Lys Asp Leu Tyr Arg F 1 5 lie lie Pro Asp Asp Arg P Asp Asp Al a Gu Gu Asp Arg Ala y Thr Leu Tyr A Ala Ala y Val Ala Pro L 70 Ala Asn Ser Ser Phe Ser C Ala Leu Ala Arg G n 100 seql i st cor r ect ed. t xt ct gac gcc atc aac gct gct ggt gtt gca hr Asp Ala lie Asn Ala Ala y Val Ala 60 iac agg ctg tct gtt gct aac tca tcg ttt sn Arg Leu Ser Val Ala Asn Ser Ser Phe 80 iac tgg acc gtg aag gcg ttg gcg cgc cag sn Trp Thr Val Lys Ala Leu Ala Arg G n 95 100 it ari cum 'he Val Asn 1 a Val Asp 25 (al Hi s G u 40 l1 a Ser Phe eu Asp Al a 3 n Leu Leu Leu Thr Val Asp Leu Al a Leu Hi s G u Asp Ar g Tr p Asp Lys Asp Al a Leu Thr Thr Leu Thr SIe Ser Val <210> 49 <211> 2898 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(2875) <223> RXN03028 <400> 49 ctaattccaa gccgagctga aaaagtctgg aagttttgcc caataagggc gttaaagtgg gtgaaagcga atttagaaat aaagaattaa ggggagagac atg ttc gag agg ttt 115 IVet Phe G u Arg Phe 1 acc gat cgt gca cgc cgc gtg att gtg ctc gcg cag gaa gag gcg cgc 163 Thr Asp Arg Ala Arg Arg Val lie Val Leu Ala G n u u Ala Arg 15 atg ctc aac cac aat tac atc ggc acg gag cac att ctc ctc ggc ctc 211 Iet Leu Asn His Asn Tyr Ile c y Thr c u His lie Leu Leu c y Leu 30 Page 59 seql i st cor r ect ed. t xt at t SIle at t SIle ggc G y aag Lys t ac Tyr ggc G y gt g Val ggc Gy 150 gtt Val ggc Gy acc Thr aag Lys aac Asn 230 gaa G u aag Lys t cc Ser gag G u gag G u ct g Leu cag G n ct g Leu ggt Sy gct Al a 120 cag G n t cc Ser gca Al a cgt Ar g gct Al a 200 at c SIle gtt Val ctt Leu aag Lys t ac Tyr 280 aac Asn gag G u gcc Al a acc Thr ct c Leu gag Su cag G n gtt Val gag G u gct Al a 170 acc Thr aag Lys cgc Ar g at t SIle ct a Leu 250 gtt Val ggt Sy cgc Ar g gtt Val cgc Ar g ggc G y ct c Leu ct g Leu ct g Leu cag G n 140 ggc G y cct Pr o ttg Leu ggc G y at g Met 220 gag G u at t SIle t cc Ser ttc Phe gac Asp 300 gca gcc aag Al a Al a Lys 45 cag gaa gt c G n G u Val cac att cct His Ile Pro cgc gaa ggc Arg G u y 95 ctt ctc ggt Leu Leu G y 110 gtc aag ctt Val Lys Leu 125 ctt ctc tcc Leu Leu Ser cag ggc gcc Sn G y Al a ggc ggt cgt G y G y Ar g 175 gtc ctt gac Val Leu Asp 190 aag ctg gat Lys Leu Asp 205 cag gtg ctc G n Val Leu cca ggt gtt Pro G y Val gtt aac ggc Val Asn y 255 ctt gac tta Leu Asp Leu 270 gaa gag cga G u G u Ar g 285 at c at c ct g Ile i e Leu gct Al a gaa G u ttt Phe 80 ct a Leu ttg Leu ggt G y ggc G y cct Pr o 160 cca Pr o cag G n cca Pr o t cc Ser ggt G y 240 aag Lys ggt G y ct g Leu ttg gaa tcc Leu G u Ser gag at t at c G u li e Ile act cca cgt Thr Pro Ar g caa atg gga G n bt G y at c cgt gag lie Arg G u 115 gct gat ctg Al a Asp Leu 130 tac gaa ggt Tyr G u G y 145 act ggc ggt Thr G y G y tct tcg ggc Ser Ser G y ttc ggc cgc Phe G y Ar g 195 gtt gtt ggt Val Val G y 210 cgt cgt acc Arg Arg Thr 225 aag acc gca Lys Thr Al a gtt cca gag Val Pro u tcc ctg gtt Ser Leu Val 275 aag aag gtc Lys Lys Val 290 at g Met ggc G y gcc Al a cac Hi s 100 ggc G y cca Pr o ggc G y gac Asp agc Ser 180 aac Asn cgc Ar g aag Lys gtt Val acc Thr 260 gca Al a ct c Leu gga G y cag G n aag Lys aag Lys gag G u cgc Ar g cag G n gct Al a 165 cca Pr o ct c Leu gat Asp aac Asn gtt Val 245 ct c Leu ggt G y aag Lys 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 ttt atc gat gag atc cac Phe Ile Asp u Ile His 305 Page seql i st cor r ect ed. t xt acc Thr 310 ct g Leu acc Thr gag G u acc Thr cgc Ar g 390 gat Asp at c SIle t cc Ser aag Lys cgc Ar g 470 cag G n cag G n ct c Leu cac Hi s gcg Al a 550 gt g Val aag Lys ct g Leu cgt Ar g 360 gag G u t cc Ser t ac Tyr gag G u ct c Leu 440 gca Al a aag Lys cgc Ar g gca Al a gaa G u 520 cgc Ar g cgt Ar g gca Al a aag Lys 330 gag G u cag G n ttg Leu acc Thr aac Asn 410 ggc Gy gag G u at c l Ie cgc Ar g ggc G y 490 gt a Val gaa G u at c SIle acc Thr ggt Gy 315 ctt Leu tac Tyr cca Pr o aag Lys gat Asp 395 gac Asp gcc Al a gtt Val gat Asp aag Lys 475 gac Asp ct g Leu tct Ser gga G y cgt Ar g 555 gca gca gaa ggc Al a Al a G u G y gcc Al a cgt Ar g gt g Val ggt G y 380 ggt G y cgc Ar g cgc Ar g gat Asp gct Al a 460 ct c Leu ct c Leu gcc Al a t ca Ser cag G n 540 gca Al a ggt G y cac Hi s 350 gtt Val cgc Ar g ctt Leu ttg Leu cgc Ar g 430 cgt Ar g gac Asp gaa G u gac Asp tgg Tr p 510 ct g Leu gaa G u ct g Leu gaa G u 335 at t SIle cca Pr o gac Asp act Thr cca Pr o 415 at c SIle at c SIle ttt Phe gag G u at c SIle 495 act Thr ct c Leu gct Al a aag Lys ggc Gy 575 gca Al a 320 ct g Leu gaa G u gag G u cgc Ar g gca Al a 400 gat Asp aag Lys gct Al a gag G u cgt Ar g 480 gct Al a ggt G y aac Asn gt c Val gat Asp 560 gtt Val at c gac gct I le Asp Al a cag acc att Sn Thr lle aag gac gca Lys Asp Al a 355 cct tcg gtt Pro Ser Val 370 t ac gaa gct Tyr G u Al a 385 gca gct cag Al a Al a G n aag gcc gtt Lys Al a Val cgc atg acc Arg IVet Thr 435 gat gtt cgc Asp Val Ar g 450 aag gca gca Lys Al a Al a 465 tca gag aag Ser G u Lys gag gtt ggc G u Val y att cct gtc lie Pro Val 515 at gaa gaa vt G u G u 530 aag gct gtc Lys Al a Val 545 cct aag cgt Pro Lys Arg ggt aag acc G y Lys Thr gcc Al a ggt G y 340 gct Al a gat Asp cac Hi s ctt Leu gac Asp 420 gca Al a cgt Ar g ggt G y gaa G u gaa G u 500 ttc Phe gag G u t cc Ser cct Pr o gag G u 580 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 ggc tcc ttc atc ttc gct ggt cca tcc Gy Ser Phe lie Phe Ala G y Pro Ser 570 Page 61 seql i st cor r ect ed. t xt t cc Ser caa G n ttc Phe acc Thr 630 at c SIle gaa G u aac Asn aag Lys gct Al a 710 cac Hi s cac Hi s ggt Sy act Thr ct g Leu 790 cag G n gt c Val cgt Ar g gct Al a gac Asp 600 gcc Al a aag Lys aag Lys ggt Sy gt c Val 680 gtt Val t ac Tyr cgc Ar g ct c Leu gtt Val 760 aag Lys gca Al a t cc Ser gtt Val aag Lys 840 ct c gca gc Leu Al a G 585 atg ggt gE Mvt G y G cct ccg gc Pro Pro G gtt cgc cc Val Arg Ar 6, gcc cac ae Al a Hi s L 650 cgc ctt ac Arg Leu TI 665 ctg atc tt Leu IIe PR ggc ctg gc G y Leu G gac cgc at Asp Arg IV 71 cct gag tt Pro G u PI 730 acc aag ge Thr Lys As 745 t cc aac gc Ser Asn Al gcc aag ge Al a Lys As cga cca tt Arg Pro Le 7£ gag aag at G u Lys II 810 gac gtc ge Asp Val G 825 ttc acc tt Phe Thr Pr ttc ctc ttc y Phe Leu ttc Phe t ac Tyr 620 aag Lys gag G u gat Asp acc Thr ttc Phe 700 aag Lys ct g Leu cag G n ct g Leu ct c Leu 780 cgt Ar g ct c Leu ggc G y aca Thr cac Hi s 605 gtt Val cca Pr o at c SIle ggt G y t cc Ser 685 t cc Ser aac Asn aac Asn at c SIle gct Al a 765 ct g Leu cgc Ar g ttc Phe tgg Tr p cca Pr o 845 Phe 590 gac Asp G y ttc Phe t ac Tyr cag G n 670 aac Asn gga G y aag Lys cgt Ar g gtt Val 750 gag G u gct Al a acc Thr ggt G y gac Asp 830 cgt Ar g ggt gac gat gat G y Asp Asp Asp cgc Ar g t ac Tyr t cc Ser aac Asn 655 gga G y ct g Leu t cc Ser gt c Val at t SIle 735 cag G n aag Lys aac Asn at c SIle gaa G u 815 ggc G y cca Pr o ttc Phe gaa G u gtt Val 640 acc Thr cgc Ar g ggc Gy t cc Ser cac Hi s 720 gat Asp at g Met gac Asp cga Ar g cag G n 800 at c SIle gag G u aag Lys gcg Al a 610 ggt G y ctt Leu ct g Leu gt g Val gct Al a 690 act Thr gag G u at c SIle gac Asp agc Ser 770 ttc Phe gaa G u gca Al a aag Lys ct c Leu cga Ar g cag G n gac Asp gt g Val 660 ttc Phe at c l Ie agc Ser aag Lys gt c Val 740 ctt Leu gaa cca Pr o gaa G u gag G u 820 acc Thr at c SIe ctt Leu ct g Leu gaa G u 645 ttg Leu aag Lys t cc Ser gat Asp aag Lys 725 ttc Phe at c SIe ct g Leu gtt Val gac Asp 805 at c SIe gac Asp 1891 1939 1987 2035 2083 2131 2179 2227 2275 2323 2371 2419 2467 2515 2563 2611 2659 atg cca gaa ggt Iet Pro G u G y 850 Page 62 aag Ly s g at As p 870 at t I Ie ac c Thr gga O31y t ct Ser gca A a ct t Le u at t I Ie act Thr 920 at c I Ie g ac As p ac c Thr 890 c ag O31n cca Pr o gt c ValI 860 g at As p g aa O31u t cc Ser aag Ly s seqI i st car r ect ed. t xt gag gct gcg gaa gca at t caa gat gt a O3 u A a A a 03 u A a I I e 03 n Asp Val 865 gt c cca gaa acc gat t ca ct t t cc gac Val Pr o O31u Thr Asp Ser Leu Ser Asp 880 885 aag t tt gag gaa gat gt a gaa aac ggc Lys Phe O31u O31u Asp Val O31u Asn O31y 895 900 ggt gac t ac t ac ggc acc gat gat cag O3 y Asp Tyr Tyr 03 y Thr Asp Asp 03 n 910 915 gag t agcaacct t t tgaaaaagg gcc O31u 925 2707 2755 2803 2851 2898 <210O> <21 1> 925 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ari cumn <400> I'bt Phe O31u Arg Phe Thr O31n Le u O31u Th r O31n I Ie A a Ty r 145 Th r Ser Ph e O31u Le u Ser I Ie Ar g O31y O31u 115 Le u O31y O31y O31y Ar g 1 95 Ar g Le u O31y O31n Ly s Ly s O31u Ar g O31n A a 1 65 Pr o Le u As p Le u Hi s Ser Ser ValI I Ie ValI Ar g 135 O31y O31y O31u O31n Ar g As n O31u 40 Le u O31n Le u O31y A a 120 O31n Ser A a Ar g A a 200 A a Hi s O31y As p Pr o O31u Th r 1 05 A a O31n Pr o O31y Ser 1 85 A a Ar g 10 As n O31u A a Th r Le u 90 O31u O31n ValI O31u A a 1 70 Th r Ly s Ar g Ty r O31y ValI Th r Ser Ph e ValI I Ie O31y 1 A a Ser As p I Ie O31y A a O31n Hi s Ar g Le u ValI 125 Le u O31n O31y ValI Ly s 205 ValI Th r A a O31u I Ie O31u Le u 110 Ly s Le u O31y O31y Le u 1 Le u Le u O31u Ly s ValI Pr a O31y O31y Le u Ser A a Ar g 1 As p As p Val Val Oy Arg Asp Lys Ou lieOu Arg Ilie N/bt On Val Leu Ser Page 63 seql i st cor r ect ed. t xt Ar g 225 Lys Val Ser Lys SIle 305 I le G n Lys Pr o Tyr 385 Al a Lys Ar g Asp Lys 465 Ser G u SIle Met Lys 545 Pr o Thr Lys Al a Val G u Thr 260 Val Ala 275 Val Leu G u lie Al a Al a I e G y 340 Al a Al a 355 Val Asp Al a Hi s G n Leu Val Asp 420 Thr Al a 435 Arg Arg Al a G y Lys G u G y G u 500 Val Phe 515 G u G u Val Ser Arg Pro 215 Asn Asn Pro Val 230 Val G u G y Leu 245 Leu Lys Asp Lys Sy Ser Arg Tyr 280 Lys G u Ile Asn 295 His Thr Leu Val 310 Ser Leu Leu Lys 325 Ala Thr Thr Leu Leu G u Arg Arg 360 Leu Thr Val G u 375 His Arg Val Ser 390 Ala Asp Arg Tyr 405 Leu Ile Asp G u Pro Ser Ser Leu 440 G u Lys G u Al a 455 Leu Arg Asp Lys 470 Lys G n Trp Arg 485 G u G n Ile Al a Lys Leu Thr G u 520 Leu His Lys Arg 535 Arg Ala lie Arg 550 Ser G y Ser Phe 565 220 G u Pro G y Val Leu I le G y 235 Ala Leu Asp 250 G n Val Tyr 265 Arg G y Asp G n Arg G y G y Al a G y 315 Pro Lys Leu 330 Asp G u Tyr 345 Phe G n Pro Ile Leu Lys Ile Thr Asp 395 Ile Asn Asp 410 Al a G y Al a 425 Arg u Val Al a lie Asp G u Arg Lys 475 Ser G y Asp 490 G u Val Leu 505 Al a G u Ser lie l e G y Arg Thr Arg 555 Il e Phe Al a 570 SIle Ser Phe Asp 300 Al a Al a Ar g Val Gy 380 Gy Ar g Ar g Asp Al a 460 Leu Leu Al a Ser G n 540 Al a Gy Val Leu G u 285 I le Al a Ar g Lys G n 365 Leu Al a Phe Met G u 445 G n Gy G u Asn Ar g 525 Asp Soy Pr o Gy 255 Leu Ar g Leu Gy G u 335 I le Pr o Asp Thr Pr o 415 I e I e Phe G u SIle 495 Thr Leu Al a Lys 5y 575 Gy 240 Lys Gy Leu Phe Al a 320 Leu G u G u Ar g Al a 400 Asp Lys Al a G u Ar g 480 Al a Gy Asn Val Asp 560 Val Page 64 seql i st cor r ect ed. t xt Sy Lys Thr G u Leu Ser Lys Ala Leu Ala y Phe Leu Phe G y Asp 590 580 Asp Thr G u 625 Val Leu SIle Thr G u 705 Asp G u Val lbet Gy 785 Ar g Gy Ser Pr o Al a 865 Asp Asp Gy Ser 595 Ser Gy Phe G n Asp 675 Asp Asp Leu Val Leu 755 I le Asp SIle Gy Asp 835 Pr o G n Leu G u Asp 915 SIle Leu Leu G u 645 Leu Lys Ser Asp Lys 725 Phe SIle Leu Val Asp 805 I le Asp Gy Val Asp 885 Gy G n G n Phe Thr 630 I le G u Asn Lys Al a 710 Hi s Hi s Gy Thr Leu 790 G n Val Ar g Lys Asp 870 I le Thr Gy SIle Gy 615 G u G u Asp Thr Al a 695 G n Phe G n Ar g G u 775 Sy Met Thr Al a Phe 855 Ser Asp Asp Sy Asp 600 Al a Lys Lys Sy Val 680 Val Tyr Ar g Leu Val 760 Lys Al a Ser Val Lys 840 Ser Al a Leu SIle Thr 920 585 let Pr o Val Al a Ar g 665 Leu Sy Asp Pr o Thr 745 Ser Al a Ar g G u Asp 825 Phe G u Al a G u Asp 905 Al a G u Sy Ar g 635 Lys Thr Phe Sy let 715 Phe Asp Al a Asp Leu 795 I le G u Phe Ser Sy 875 Leu Val Ser Hi s 605 Val Pr o SIle Sy Ser 685 Ser Asn Asn SIle Al a 765 Leu Ar g Phe Tr p Pr o 845 G u Val Lys Sy G u 925 Asp Sy Phe Tyr G n 670 Asn Sy Lys Ar g Val 750 G u Al a Thr Sy Asp 830 Ar g Al a Pr o Phe Asp 910 Ar g Tyr Ser Asn 655 Sy Leu Ser Val SIe 735 G n Lys Asn SIe G u 815 Sy Pr o Al a G u G u 895 Tyr <210> 51 Page seqi i st car r ect ed. t xt <21 1> 1104 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1081) <223> FRXA02470 <400> 51 ct aat tccaa gccgagct ga aaaagt ct gg aagtt t tgcc gt gaaagcga at t tagaaat aaagaat taa ggggagagac caat aagggc gt taaagt gg ac c Thr at g vb t at t I Ie at t O31y aag Ly s t ac Tyr O31y gt g ValI O31y 1 50 gt t ValI O31y ac c Thr cgt Ar g aac As n gag G u ct g Le u c ag O3n ct g Le u ggt O3y gct A a 120 c ag O3n t cc Ser gca A a cgt Ar g gct A a 200 cgc Ar g aat As n gag G u gcc A a ac c Thr ct c Le u gag O3u c ag O3n gt t ValI gag (3u gct A a 1 70 ac c Thr aag Ly s cgc Ar g t ac Tyr G y gt c ValI acc Thr 75 ag c Ser t t c Ph e gt c ValI at t G3y 155 gct A a t ct Ser gac As p gt g ValI at c I Ie gt t ValI cgc Ar g ggc G31y ct c Le u ct g Le u ct g Le u c ag O31n 140 ggc O31y cct Pr o tt g Le u ggc (31y at t O31y gca A a 45 c ag O31n c ac Hi s cgc Ar g ct t Le u gt c ValI 125 ct t Le u c ag O31n O31y gt c ValI aag Ly s 205 gt g ValI ac g Thr gcc A a g aa O31u at t I Ie g aa O31u ct c Le u 110 aag Ly s ct c Le u ggc O31y ggt O31y ct t Le u 1 90 ct g Le u gcg A a c ac Hi s gct A a g aa O31u tt t Ph e 80 ct a Le u tt g Le u ggt O31y ggc O31y cct Pr o 1 60 cca Pr o c ag O31n cca Pr o at g vb t 1 c ag O31n at t I Ie tt g Le u gag O31u act Thr c aa O31n at c I Ie gct A a t ac Tyr 145 act Thr t ct Ser tt c Ph e gt t ValI g aa O31u ct c Le u g aa (31u at t I Ie cca Pr o at g vb t cgt Ar g g at As p 130 g aa O31u O31y t cg Ser ggc O31y gt t ValI 210 ttc gag Phe 03 u gag O31u ct c Le u t cc Ser at c I Ie cgt Ar g gga O31y gag O31u 115 ct g Le u ggt O31y ggt O31y ggc O31y cgc Ar g 1 ggt O31y agg ttt Ar g Phe gcg cgc Ala Ar g ggc ct c O3 y Leu at g gga !vb t (1y ggc cag O31y (1n gcc aag A a Lys cac aag Hi s Lys 1 00 ggc gag O31y (1u cca cgc Pr o Ar g ggc cag O31y (1n gac gct As p A a 1 agc cca Ser Pr o 1 aac ct c Asn Leu cgc gat Ar g As p Page 66 IND seqI i st car r ect ed. t xt aag gaa atc gag cgc at c at g cag gt g ct c t cc cgt cgt acc aag aac 787 SLys (1uIlIe (1u ArglIle/b t (1n VaILeu SerAr gAr gThr Lys Asn 215 220 225 fl aac cca gt t ct t at t ggt gag cca ggt gt t ggt aag acc gca gt t gt t 835 a.Asn Pr o Val Leu Ilie 31y G31u Pro G31y Val G31y Lys Thr Al a Val Val S230 235 240 245 gaa ggt ct t gca ct a gac at t gt t aac ggc aag gt t cca gag acc ct c 883 (31u G31y Leu Al a Leu Asp Ilie Val Asn G31y Lys Val Pr o (31u Thr Leu 250 255 260 C1 aag gac aag cag gt t t ac tcc ctt gac t taggt t cc ct ggtt gca ggt 931 SLys Asp Lys O31n Val Tyr Ser Leu Asp Leu G31y Ser Leu Val Al a G31y 00 265 270 275 t tcc cgt t ac cgc ggt gac t tc gaa gag cga ct g aag aag gt c ct c aag 979 C1 Ser Ar g Tyr Ar g G31y Asp Phe O31u O31u Ar g Leu Lys Lys Val Leu Lys ID280 285 290 Sgag at t aac cag cgc ggc gac at c at c ct g t tt at c gat gag at c cac 1027 (31 Ou Ile Asn (1n Ar g 1y Asp Ilie Ilie Leu Phe Ile Asp (1u Ilie Hi s 295 300 305 acc ct C gt g ggt gca ggt gca gca cga agg cgc aat cga cgc t gc ct C 1075 Thr Leu Val G y Al a G y Al a Al a Ar g Ar g Ar g Asn Ar g Ar g Cys Leu 310 315 320 325 cct gct t aagccaaag ct t gcccgcg gt g 1104 Pr o Al a <210> 52 <211> 327 <212> PRT <213> Cor ynebact er i urn gi ut anm cumn <400> 52 I'b t Phe O31u Ar g Ph e Thr As p Arg Ala Arg Ar g Val IIIe ValI L eu Al a 1 5 10 O31n (1u 1u Al aAr g I'bt Leu Asn Hi sAsn Ty r IIe 1y Th r O31u Hi s 25 Ilie Leu Leu (1y Leu Ilie His Ou~y (1u 1y ValI Aa AaLys Al a 40 Leu O31u Se r /b t G31y I Ie Ser Leu Asp Al a Val Arg O31n O31u Val O31u 55 O3 u Ilie Ilie 03 y O31n 03 y Se r O31n Pr o Thr Thr 03 y Hi s Ilie Pr o Phe 70 75 Thr Pr o Ar g Al a Lys Lys Val Leu O31u Leu Ser Leu Ar g O31u O31y Leu 90 O31n 'b t O31y Hi s Lys Tyr Ilie O31y Thr O31u Phe Leu Leu Leu O31y Leu 100 105 110 Ilie Ar g O31u O31y O31u O31y Val Al a Al a O31n Val Leu Val Lys Leu O31y 115 120 125 Al a Asp Leu Pr o Ar g Val Arg O31n O31n Val Ilie O31n Leu Leu Ser G31y 130 135 140 Tyr (3u (3y (3y (31n 31y G31y Ser Pr o 31u G31y 31y O31n G31y Al a Pr o 145 150 155 160 Page 67 Thr Ser Ph e ValI Ar g 225 Ly s ValI Ser Ly s I Ie 305 As n G31y G31y Ar g 1 95 G31y Thr Al a (31u ValI 275 ValI O31u Ar g Al a 1 65 Pr o Le u As p As n ValI 245 Le u G31y Ly s Hi s Le u 325 G31y O31u O31n (31u 215 Pr o G31y As p Ar g I Ie 295 Le u Al a seql i st car r ect ed. t xt Ala Gy Ala Ala Pro Gy 1 70 Ar g Ser Thr Ser Leu Val 1 Al a Al a Lys As p 03 y Lys 200 205 li1e O31u Ar g I Ie 'b t O31n 220 Val Leu I Ie 31y O31u Pr o 235 Leu Al a Leu As p I I e Val 250 Lys 03 n Val Ty r Ser Leu 265 Ty r Ar g G31y As p Phe O31u 280 285 As n G n Ar g G y As p I Ie 300 Val 31y Ala G31y Al a Al a 315 Ar g 1 As p As p Le u ValI G31y 255 Le u Ar g Le u Ar g <210O> 53 <21 1> 1956 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 OIDS <222> (1933) <223> FRXA02471 <400> 53 ggt gact tcg aagagcgact gaagaaggt c ct caaggaga at cct gt t t a t cgat gagat ccacaccct c gt gggt gcag gca Al a ct g Le u g aa O31u gag O31u g ac As p ac c Thr g ac As p t cg Ser gcc Al a ggt G31y gct Al a g at As p t cc Ser gca Al a ct t Le u ct c Le u ct g Le u ac c Thr gag O31u ac c Thr aag Ly s ct g Le u cgt Ar g gag O31u cca aag Pr o Lys 15 gat gag As p (1u ttc cag Phe 03 n at c tt g I I e Leu Page 68 t taaccagcg cggcgacat C gtg cag cac gaa ggc 115 Val O31n Hi s 31u G31y 1 ctt gcc cgc ggt gaa 163 Leu Al a Ar g G31y O31u t ac cgt aag cac at t 211 Tyr Ar g Lys Hi s Ilie cca gt g cag gt t cca 259 Pr o Val O31n Val Pr o aag ggt ct g cgc gac 307 Lys G31y Leu Arg Asp seql i st cor r ect ed. t xt cgc tac gaa gct cac cac cgc gta tcc Arg Tyr u Ala His His Arg Val Ser at c acc gat ggt gct ctt act I e Thr Asp y Ala Leu Thr gca Al a gat Asp aag Lys gct Al a gag G u 150 cgt Ar g gct Al a ggt Gy aac Asn gt c Val 230 gat Asp gtt Val gac Asp ttc Phe gaa 31u 310 gct Al a gcc Al a at g Met 120 gtt Val gca Al a gag Gu gtt Val cct Pr o 200 gaa G u gct Al a aag Lys aag Lys gat Asp 280 gcg Al a ggt Gy ctt Leu gac Asp gca Al a cgt Ar g ggt Gy gaa Gu 170 gaa G u ttc Phe gag G u t cc Ser cct Pr o 250 gag G u ct c Leu cga Ar g cag G n gct Al a ct c Leu cct Pr o gag G u ctt Leu 155 aag Lys gag G u aag Lys tt g Leu cgt Ar g 235 t cc Ser ct g Leu at c SIle ctt Leu ct g Leu 315 gat Asp at c SIle t cc Ser aag Lys 140 cgc Ar g cag G n cag G n ct c Leu cac Hi s 220 gcg Al a ggc Gy t cc Ser caa G n ttc Phe 300 acc Thr t ac Ty r gag Su 110 ct c Leu gca Al a aag Lys cgc Ar g gca Al a 190 gaa G u cgc Ar g cgt Ar g ttc Phe gct Al a 270 gac Asp gcc Al a aag Lys at c I e 95 gct Al a cgc Ar g gcg Al a gag G u t cc Ser 175 gaa G u gct Al a at c SIle cgt Ar g at c SIle 255 ct c Leu at g Met cct Pr o gtt Val aac Asn ggc Gy gag G u at c lile cgc Ar g 160 ggc Gy gt a Val gaa G u at c SIle acc Thr 240 ttc Phe gca Al a ggt Gy ccg Pr o cgc Ar g 320 cac Hi s gac Asp gcc Al a gtt Val gat Asp 145 aag Lys gac Asp ct g Leu tct Ser gga Gy 225 cgt Ar g gct Al a gga Gy gag G u gga Gy 305 cgt Ar g aag Lys cgc Ar g cgc Ar g gat Asp 130 gct Al a ct c Leu ct c Leu gcc Al a t ca Ser 210 cag G n gca Al a ggt Gy ttc Phe ttc Phe 290 t ac Tyr aag Lys gag G u ttc Phe at g Met 115 gag G u cag G n ggc Gy gag Gu aac Asn 195 cgc Ar g gat Asp ggt Gy cca Pr o ct c Leu 275 cac Hi s gtt Val cca Pr o at c SIle ttg Leu 100 cgc Ar g cgt Ar g gac Asp gaa G u gac Asp 180 tgg Tr p ct g Leu gaa G u ct g Leu t cc Ser 260 ttc Phe gac Asp ggc Gy ttc Phe t ac Tyr 340 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 gtt gtg ctt ttc Val Val Leu Phe gac gaa at Asp G u II 330 c gag aag gcc e G u Lys Al a 335 Page 69 seql i st cor r ect ed.t t ggt cgc ctt acc acc ttg ctg cag gtg ttg gaa ga Thr Leu Leu G n Val Leu G u As 345 p cgc Ar g ggc Gy t cc Ser 390 cac Hi s gat Asp at g Met gac Asp cga Ar g 470 cag G n at c SIle gag G u aag Lys gaa G u 550 acc Thr gaa G u t ac Tyr gt g Val 360 gct Al a act Thr gag G u at c SIle gac Asp 440 agc Ser ttc Phe gaa G u gca Al a aag Lys 520 at g Met at t SIle t ca Ser gt a Val acc Thr 600 ttc Phe at c l Ie agc Ser aag Lys 410 gt c Val ctt Leu gaa G u cca Pr o gaa G u 490 gag G u acc Thr gaa G u gat Asp t cc Ser 570 aac Asn gat Asp aag Lys t cc Ser gat Asp 395 aag Lys tt c Phe at c SIle ct g Leu gtt Val 475 gac Asp at c SIle gac Asp ggt G y gt a Val 555 gac Asp ggc Gy cag G n aac Asn aag Lys 380 gct Al a cac Hi s cac Hi s ggt Gy act Thr 460 ct g Leu cag G n gt c Val cgt Ar g aag Lys 540 gat Asp at t SIle acc Thr gga G y u y 350 gt c Val gtt Val t ac Tyr cgc Ar g ct c Leu 430 gtt Val aag Lys gca Al a t cc Ser gtt Val 510 aag Lys tct Ser gca Al a ctt Leu at t SIle 590 act Thr Arg Leu Inr ct g Leu ggc G y gac Asp cct Pr o 415 acc Thr t cc Ser gcc Al a cga Ar g gag G u 495 gac Asp ttc Phe gag G u gct Al a gaa G u 575 gat Asp gct Al a at c SIle ct g Leu cgc Ar g 400 gag G u aag Lys aac Asn aag Lys cca Pr o 480 aag Lys gt c Val acc Thr at c SIle gac Asp 560 acc Thr cag G n cca Pr o ttc Phe ggc G y 385 at g Met ttc Phe gat Asp gca Al a gac Asp 465 ttg Leu at c SIle gaa G u ttc Phe tct Ser 545 ggc G y ctt Leu gt g Val agc Ser :xt gat ggt cag gga Asp G y On y 355 acc tcc aac ctg Thr Ser Asn Leu 370 ttc tcc gga tcc Phe Ser G y Ser aag aac aag gtc Lys Asn Lys Val 405 ctg aac cgt att Leu Asn Arg lie 420 cag atc gtt cag Sn Ile Val n 435 ctg gct gag aag Leu Al a G u Lys 450 ctc ctg gct aac Leu Leu Ala Asn cgt cgc acc atc Arg Arg Thr Ile 485 ctc ttc ggt gaa Leu Phe G y G u 500 ggc tgg gac ggc Sy Trp Asp y 515 aca cca cgt cca Thr Pro Arg Pro 530 gtc gag gct gcg Val G u Ala Ala gat gtc cca gaa Asp Val Pro G u 565 gaa aag ttt gag G u Lys Phe G u 580 tcc ggt gac tac Ser G y Asp Tyr 595 aag gag Lys G u 610 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1933 Page IN seql i st cor r ect ed. t xt Stagcaacctt ttgaaaaagg gcc 1956 <210> 54 <211> 611 <212> PRT <213> Corynebacter i um gl ut am cum <400> 54 Val G n His G u Gy Ala IIe Asp Ala Ala Ser Leu Leu Lys Pro Lys 1 5 10 C( Leu Ala Arg G y u Leu n Thr lie y Ala Thr Thr Leu Asp u 25 00 0 Tyr Arg Lys His lie G u Lys Asp Ala Ala Leu G u Arg Arg Phe G n 40 CO Pro Val G n Val Pro O u Pro Ser Val Asp Leu Thr Val G u Ile Leu 55 Cq Lys G y Leu Arg Asp Arg Tyr G u Ala His His Arg Val Ser lie Thr 70 75 Asp y Ala Leu Thr Al a Ala Ala G n Leu Ala Asp Arg Tyr I e Asn 90 Asp Arg Phe Leu Pro Asp Lys Ala Val Asp Leu I e Asp G u Ala y 100 105 110 Ala Arg IVet Arg lie Lys Arg IVet Thr Ala Pro Ser Ser Leu Arg G u 115 120 125 Val Asp G u Arg ie Al a Asp Val Arg Arg G u Lys G u Ala Ala lle 130 135 140 Asp Ala G n Asp Phe G u Lys Ala Ala y Leu Arg Asp Lys G u Arg 145 150 155 160 Lys Leu G y G u G u Arg Ser G u Lys G u Lys G n Trp Arg Ser G y 165 170 175 Asp Leu Gu Asp Ile Ala Gu Val Gy Gu Gu Gn Ile Ala Gu Val 180 185 190 Leu Ala Asn Trp Thr G y lie Pro Val Phe Lys Leu Thr G u Ala G u 195 200 205 Ser Ser Arg Leu Leu Asn Iet G u u G u Leu His Lys Arg lie lie 210 215 220 Gy G n Asp G u Ala Val Lys Ala Val Ser Arg Ala lie Arg Arg Thr 225 230 235 240 Arg Ala y Leu Lys Asp Pro Lys Arg Pro Ser y Ser Phe lie Phe 245 250 255 Ala y Pro Ser y Val y Lys Thr G u Leu Ser Lys Ala Leu Ala 260 265 270 G y Phe Leu Phe G y Asp Asp Asp Ser Leu Ile n Ile Asp IVet G y 275 280 285 G u Phe His Asp Arg Phe Thr Ala Ser Arg Leu Phe G y Ala Pro Pro 290 295 300 G y Tyr Val G y Tyr G u G u G y y G n Leu Thr G u Lys Val Arg 305 310 315 320 Page 71 seqi i st car r ect ed. t xt Ar g Lys Pr o Phe Ser Val Val Leu Phe G u I Ie G u Lys Al a Hi s 335 Ly s Th r Ph e G31y 385 vb t Ph e As p Al a As p 465 Le u I Ie (31u Ph e Ser 545 G31y Le u ValI Ser 325 I Ie G31y 355 Ser Ser As n As n I Ie 435 Al a Le u Ar g Ph e Tr p 515 Pr o (31u ValI Ly s G31y 595 (31u As n G31y Le u Ser ValI 405 li1e O31n Ly s As n I Ie 485 O31u G31y Pr o Al a O31u 565 O31u Ty r Le u I Ie Th r 375 O31u As p O31u ValI vb t 455 G31y Ar g G31y Ser Pr o 535 Al a As p As p G31y O31n 345 As p As p As p Le u ValI 425 Le u I Ie As p I Ie G31y 505 As p Pr o O31n Le u O31u 585 As p Le u Ly s Ser As p 395 Ly s Ph e I Ie Le u ValI 475 As p I Ie As p G31y ValI 555 As p G31y O31n O31u As n Ly s 380 Al a Hi s Hi s G31y Th r 460 Le u O31n ValI Ar g Ly s 540 As p I Ie Th r G31y As p Th r 365 Al a O31n Ph e O31n Ar g 445 (31u O31y vb t Th r Al a 525 Ph e Ser As p As p O31y 605 Ar g Le u O31y As p Pr a 415 Th r Ser Al a Ar g O31u 495 As p Ph e O31u Al a O31u 575 As p Al a <210O> <21 1> 1446 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (1423) <223> RXA02630 Page 72 seql i st cor r ect ed. t xt <400> gcgggttcga aaatgtcgat gattaaacca ctaaagagct cacaggaagt gttcagacta cttagagtga cgccccagcc acagggttca taatcaaatc atg aca aat caa ttc Met Thr Asn G n Phe ccc Pr o gaa G u aac Asn gaa G u agt Ser aag Lys tct Ser tcg Ser cgc Ar g gtt Val 150 tct Ser aat Asn agt Ser cct Pr o cca Pr o 230 aac Asn aac Asn gct Al a aac Asn gat Asp aaa Lys gct Al a tct Ser 120 act Thr cct Pr o ggc Gy cac Hi s t cc Ser 200 aca Thr at g let ggt Gy ttc Phe aat Asn caa G n cag G n ggc Gy ggt Gy acc Thr gct Al a gt c Val gga Gy 170 gt g Val gga Gy at t SIle ttt Phe gag G u gaa G u caa G n caa G n cct Pr o 75 at c SIle agc Ser cca Pr o gaa G u gt c Val 155 t cc Ser gca Al a act Thr gct Al a gga G3y 235 aac Asn cat Hi s aac Asn gga Gy ct a Leu gga Gy gtt Val gtt Val cca Pr o 140 tct Ser at t SIle ggc Gy aca Thr gt g Val 220 gat Asp ccg Pr o gt g Val ccc Pr o 45 aat Asn agc Ser acg Thr gt g Val aat Asn 125 ggt Gy at t SIle at t SIle at t SIle gcg Al a 205 at c SIle tcg Ser gac Asp cgt Ar g t at Tyr gag G u aca Thr gca Al a ggt Gy 110 gct Al a t ca Ser cag G n t cc Ser gaa G u 190 caa G n aag Lys gac Asp cgt Ar g 15 agt Ser cct Pr o caa G n gat Asp ct c Leu 95 gtt Val ctt Leu gcg Al a gcc Al a tct Ser 175 caa G n gct Al a at t SIle gca Al a 1 gca t cg Al a Ser t ca t at Ser Tyr ggt gcg G y Al a caa gct G n Al a gta aag Val Lys 80 gcg tta Al a Leu gca gca Al a Al a gag cag G u G n gaa cag G u G n 145 att act lIe Thr 160 gat ggt Asp y tct ggt Ser G y gat ttt Asp Phe agg gat Arg Asp 225 tta ggc Leu G y 240 gaa G u ccg Pr o ggc G y cca Pr o cca Pr o at g Met acc Thr ccc Pr o 130 gtt Val agg Ar g t ac Tyr gt g Val at t SIle 210 gt g Val gtt Val act Thr cag G n ttc Phe gcc Al a gct Al a ctt Leu cag G n 115 agc Ser gct Al a acg Thr gt c Val tta Leu 195 gct Al a t cc Ser gga G y cca Pr o tgg Tr p ggc G y tgg Tr p aaa Lys gtt Val 100 ctt Leu gt g Val gcc Al a tct Ser at g Met 180 gaa G u ggt G y aac Asn caa G n t ca Ser ggt G y tct Ser acc Thr gaa Gu ggt Sy ggt Sy cag G n gca Al a gct Al a 165 acc Thr gta Val gat Asp ctt Leu agt Ser 245 Page 73 IN seql i st cor r ect ed. t xt Sgtg atg gct gtt ggt tct cca ctg ggt ctg agc tcc act gtg acc acc 883 Val Met Ala Val G y Ser Pro Leu G y Leu Ser Ser Thr Val Thr Thr 250 255 260 C) ggt att gtg tcg gcc gtg aac cgt cct gtg cga gct tct ggt gat ggc 931 (G y Il e Val Ser Al a Val Asn Arg Pro Val Arg Al a Ser G y Asp G y S265 270 275 Sgga gag tcg tcc ctc atc gat gct atc cag acc gat gct gcg atc aac 979 Gy u Ser Ser Leu Ile Asp Ala lie n Thr Asp Ala Ala lie Asn 280 285 290 CK1 cct ggt aac tct ggt ggt ccg ctg gtt gat atg gat ggc aac ctc att 1027 0 Pro y Asn Ser y G y Pro Leu Val Asp Iet Asp y Asn Leu Ile 00 295 300 305 0 ggc atg aat tcg gta att gca tcg att tcg agc acc agc gat tcc gca 1075 C 31 Gy Vet Asn Ser Val Ile Ala Ser lie Ser Ser Thr Ser Asp Ser Ala \C 310 315 320 325 0 ggt tcc att ggt ctt ggt ttt tct atc cca tcc aac ttt gcc aag cgc 1123 C 1 Gy Ser Ile y Leu y Phe Ser Ile Pro Ser Asn Phe Ala Lys Arg 330 335 340 gtg gcc gat caa ttg atc agc acc ggc cag gta act cag ccg atg atc 1171 Val Al a Asp G n Leu I e Ser Thr G y G n Val Thr G n Pro IVet Ile 345 350 355 ggt gtg cag gtt ggc act gac aac tca gtg aca ggc gct gtg att gcc 1219 Sy Val n Val y Thr Asp Asn Ser Val Thr y Ala Val Ile Ala 360 365 370 agt gtt caa gat ggt gga ccg gcc gca gat gct gga ctt cag cca ggc 1267 Ser Val n Asp y y Pro Ala Ala Asp Ala y Leu n Pro y 375 380 385 gat atc gtg acc aag ctc aat gat cga gtt att gat agc cca gac tcc 1315 Asp lie Val Thr Lys Leu Asn Asp Arg Val lie Asp Ser Pro Asp Ser 390 395 400 405 ttg atc gct gct gtt cgt tcg cat gat ttt ggc gaa acc gtc act tta 1363 Leu IIe Ala Ala Val Arg Ser His Asp Phe G y G u Thr Val Thr Leu 410 415 420 aca att aca cag cca gat acc tcg cag agc cgg gag gta gag gtt act 1411 Thr lie Thr G n Pro Asp Thr Ser G n Ser Arg G u Val G u Val Thr 425 430 435 ctg acg agt gag taggtttaaa agagttaatc tgc 1446 Leu Thr Ser G u 440 <210> 56 <211> 441 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 56 IVet Thr Asn G n Phe Pro Thr Asn Asn y G u Asn Pro Asp Arg Ala 1 5 10 Ser G u Thr Pro Ser G u Thr Asn Ser Phe G u His Val Arg Ser Ser 25 Tyr Pro G n Trp y Asn Thr Ala Ser Asn n Asn Pro Tyr Pro y 40 Page 74 seql i st cor r ect ed. t xt n Thr Gn Gn Gv Asn Al a y Phe y Ser u n As Gu Gn Gn Al a Lys Leu Al a G n G n 145 Thr Sy Sy Phe Asp 225 Sy Ser Al a Asp Asp 305 Thr Asn Thr Sy Sy 385 Al a Al a Leu G n 115 Ser Al a Thr Val Leu 195 Al a Ser Sy Val Sy 275 Al a Asn Asp Al a Pr o 355 Val G n Thr G u Sy Sy G n Al a Al a 165 Thr Val Asp Leu Ser 245 Thr Sy Asn SIle Al a 325 Ar g SIle Al a Sy Tr p Ar g SIle Asp Thr 135 Leu G u Asn Phe Ser 215 Val lbet SIle G u Sy 295 Mat Ser Al a Val Val 375 I le Asp Lys Al a Ser 120 Thr Pr o Sy Hi s Ser 200 Thr lbet Al a Val Ser 280 Asn Asn SIle Asp G n 360 G n Val Asn Val Thr 105 Ser Asn Ser Ser Val 185 Asp Asp Ser Val Ser 265 Ser Ser Ser Sy G n 345 Val Asp Thr Pr o I le Ser Pr o G u Val 155 Ser Al a Thr Al a Sy 235 Ser Val SIle Sy SIle 315 Sy SIle Thr Sy Leu 395 Ser Thr Val Asn 125 Gy I e I e l e Al a 205 l le Ser Leu Ar g Al a 285 Leu Ser Ser Thr Asn 365 Al a Asp Thr Al a Sy 110 Al a Ser G n Ser G u 190 G n Lys Asp Sy Pr o 270 l le Val SIle SIle Sy 350 Ser Al a Ar g Asp Leu Val Leu Al a Al a Ser 175 G n Al a SIe Al a Leu 255 Val G n Asp Ser Pr o 335 G n Val Asp Val Asp Ser Pro Asp Ser Leu I e Al a Ala Val Arg Ser His Asp Phe G y 405 410 415 Page seql i st cor r ect ed. t xt Su Thr Val Thr Leu Thr lie Thr n Pro Asp Thr Ser n Ser Arg 420 425 430 G u Val G u Val Thr Leu Thr Ser G u 435 440 <210> 57 <211> 518 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> RXA02834 <400> 57 gat Asp 1 act Thr ggt Sy gca Al a ttt Phe tta Leu gt g Val caa G n act Thr at a SIle 145 gtt Val aaa Lys aat Asn at t SIle ttc Phe cca Pr o caa G n gaa G u ggt G y 115 at t SIle aaa Lys cct Pr o ggt aga G y Arg 5 att ggt I e G y agt gat Ser Asp aaa cct Lys Pro tta tca Leu Ser tta aat Leu Asn gaa gcg G u Al a 100 gca aga Al a Ar g gca cgt Al a Ar g gt a gat Val Asp acg tct Thr Ser 165 agt Ser gtt gac ttt Val Asp Phe aaa Lys 10 aat acc att atc Asn Thr Ile Ile at c at g I e bet t ca caa gt a Ser G n Val gat aca gag Asp Thr G u 40 gaa ata tta G u Ile Leu gtt aat gat Val Asn Asp 70 atg aga tta IVet Arg Leu aaa aaa tgg Lys Lys Trp cca tta aaa Pro Leu Lys 120 atg atg att Vet Vet lie 135 tta aat gac Leu Asn Asp 150 tta ctt gaa Leu Leu G u 25 aaa gca gtt Lys Al a Val aat cgt at g Asn Arg IVet atg agt atg et Ser ?et 75 tta gat caa Leu Asp G n 90 ct a ggt gaa Leu G y G u 105 cgc ttt gtt Arg Phe Val aaa gaa agt Lys G u Ser aat aaa gaa Asn Lys G u aat Asn at g Met gat Asp at t SI e cat Hi s gaa G u caa G n ct a Leu 140 ctt Leu gt a Val gac Asp gac Asp gt a Val at c SIle gcg Al a cga Ar g 125 cct Pr o gat Asp aaa Lys agt Ser at c SIle gat Asp t ca Ser t at Tyr 110 caa G n gaa G u ttt Phe gat Asp ct a Leu gt g Val aaa Lys at t SIle gaa G u at a SIle ggt G y aag Lys gct Al a cat Hi s tta Leu att SIe gaa G u cca Pr o gaa G u aca Thr gtt Val taatctagca aaatat t aat ttg <210> 58 <211> 165 <212> PRT <213> Corynebacteri um gl utami cum Page 76 <400> 58 Asp Ser Lys Thr Ser As n G31y G31u lIe A a Ty r Phe Phe Lys Pr o Leu Thr G3 n Val Thr G31u G3 n Phe G3 y 115 Thr Pr o I Ie 130 1 e I I e Lys 145 Val Lys Pr o Ar g G31y As p Pr o Ser As n A a Ar g Ar g As p Ser 1 ValI (31n Thr I Ie 55 As n Ar g Ly s Le u vb t 135 As n seqI i st car r ect ed. t xt Asp Phe Lys Asn Thr I Ie 10 Val Leu Leu G31u Asn Val G31u Lys Ala Val I'b t As p 40 Leu As n Ar g I'b t As p As p As p I'bt Ser I'b t I Ie Val Leu Leu As p G3 n Hi s I I e 90 Tr p Leu a31y a31u a31u Ala 1 Lys Ar g Phe Val a31n Ar g 120 125 li1e Lys a31u Ser Leu Pr o 140 As p As n Lys a3 u Leu As p 1 lIe As p Le u ValI Ly s I Ie au le ay Ly s <210O> 59 <21 1> 1314 <21 2> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1291) <223> RXA00112 <400> 59 gcgt taggct gacgggct cc ggt ggcct aa aat cgaagcg at at tatcga tt t tcgaaag cgt agaacac caaaccat tg gt c ValI ggt a31y gt t ValI ct c Le u g at As p cgt Ar g ggC a31y g at As p gt c ValI ggC a31y gt a ValI ac c Thr at c I Ie gcc A a gt t ValI gcg A a gt c ValI tt c Ph e ggC a31y ct t Le u ct c Le u ac c Thr gca A a 45 cgc Ar g gt t ValI t cg Ser gca A a tt c Ph e at g gca [vb t A a 15 ct g ct g Leu Leu gca gct A a A a ct c ct g Leu Leu Page 77 at ctaaaaat t tggcagt ta ttg agc ccc agc ctg 115 Leu Ser Pro Ser Leu 1 t tc gcc ct g t gg ggt 163 Phe Ala Leu Tr p a31y tcc acc gtc ggc gtc 211 Ser Thr Val a3 y Val cca t tt gt c at g ggt 259 Pr o Phe Val I'b t a31y gcg atc ggc acc gtg 307 A a Il e 03 y Thr Val seql i st cor r ect ed. t xt gt g Val gcg Al a ggg G y ct c Leu gga G y caa G n 150 t cc Ser gaa Gu gca Al a agc Ser gt g Val 230 acc Thr ct g Leu ccg Pr o gga G y gaa Gu 310 ct g Leu aga Ar g ggc G y gca Al a 120 aga Ar g ct a Leu ct c Leu gac Asp cgc Ar g 200 cga Ar g aac Asn at c SIle at c SIle gca Al a 280 cca Pr o at c SIle ggt Gy aac Asn at t SIle ccc Pr o t cc Ser acc Thr cca Pr o 170 ccc Pr o t cc Ser at g Met cac Hi s acc Thr 250 gt c Val act Thr t cc Ser at c SIle t at Tyr 330 ttg gga Leu G y 75 atc aaa I e Lys ttc caa Phe G n ctg gcc Leu Al a cgc atc Arg Ile 140 ctg ccc Leu Pro 155 ctg att Leu II e gaa at c Gu lle gt c at c Val lile ggt tct G y Ser 220 gtt gtt Val Val 235 cgc tcc Arg Ser ctt tac Leu Tyr ccg cta Pro Leu gga cct G y Pro 300 acc ggc Thr y 315 aat ctc at c Asn Leu Ile ggc gcc ttc Phe gt a Val aca Thr 125 ct g Leu t cc Ser t cc Ser aac Asn cac Hi s 205 ggc Gy gca Al a gca Al a agc Ser gac Asp 285 ttc Phe agc Ser cga Ar g ttg Leu 110 ggc G y ggc G y aaa Lys ccc Pr o gt c Val 190 gt g Val ttt Phe ggt Gy gag G u cct Pr o 270 act Thr aac Asn aac Asn agt Ser 95 gcc Al a ct c Leu ttt Phe at c SIle ttc Phe 175 acc Thr at g Met gt g Val acc Thr gt a Val 255 gac Asp ggc G y gcc Al a at t SIle t cc Ser 335 uy 80 t cc Ser acc Thr ccc Pr o gt a Val gct Al a 160 acc Thr aac Asn ggt Gy gca Al a t cc Ser 240 gt g Val ct c Leu gat Asp t cc Ser t ac Tyr 320 at c SI e AI a agg Ar g ttg Leu gga G y gac Asp 145 gcg Al a ggc Gy gtt Val gac Asp t cc Ser 225 acc Thr ttc Phe ggc G y gaa Gu cca Pr o 305 gcc Al a caa G n cac ttg ggt Hi s Leu G y acc tta gat Thr Leu Asp 100 atc gtg gt g Ile Val Val 115 act gt c gcc Thr Val Al a 130 aaa tac acc Lys Tyr Thr at g ct c agc IVet Leu Ser gga t cc t cg G y Ser Ser 180 gac ct a gt c Asp Leu Val 195 gcc caa gaa Al a G n u 210 ccc gac tac Pro Asp Tyr gtc agc ct g Val Ser Leu tac gac ccg Tyr Asp Pro 260 ttg gat cca Leu Asp Pro 275 gca at c gt c Al a I e Val 290 gcc agg gtc Al a Ar g Val aac ggc cag Asn G y G n gct Al a t ct Ser tgg Tr p agc Ser ccg Pro gaa G u 165 gt g Val gaa G u tgc Cys gtt Val gat Asp 245 aac Asn ct g Leu at g Met cgc Ar g cac Hi s 325 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 gaa cgc gaa gcc G u Arg G u Ala tca gtc cgc gga Ser Val Arg 3 y tct gga aac tcc Ser G y Asn Ser 340 Page 78 ggc ggc cca at g acc aac gaa at g g G31y G31y Pr o I'b t Thr Asn O31u I'b t C 345 3 ggc gca gcg atc gac ggc tcc gat a G3 y Al a Al a Il e Asp G3 y Ser Asp T 360 365 gag gt a cag gag cgg at c ggc gac a (31u Val O31n O31u Arg Ilie O31y Asp 1 375 380 gat acg at g cag t gc gcg gt t t ct t Asp Thr I'b t O31n Cys Al a Val Ser 390 395 <210> <211> 397 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> Leu Ser Pr o Ser Leu Val Val Asp A 1 5 Phe Al a Leu Tr p 03 y 03 y Tr p Ar g C Ser Thr Val G31y Val Val Ser G31y L 40 Pr o Phe Val I'bt G31y Leu Thr Asp S Ala Ilie G31y Thr Val Val Leu Leu\ 70 Al a Hi s Leu G31y Al a Al a Ilie Ar gA Ar g Thr Leu Asp Ser G31y Leu G31y P 100 1 Leu Ilie Val Val Tr p Leu Val Al a 1 115 120 G31y Thr Val Al a Ser G31y Ilie Ar gA 130 135 Asp Lys Tyr Thr Pro O31n G31y LeuA 145 150 Al a I'bt Leu Ser O31u Ser G31y Leu F 1 65 G31y G31y Ser Ser Val O31u Val Asp A 180 1 Val Asp Leu Val O31u Al a I'bt Ar g F 195 200 Asp Al a O31n O31u Cys Ser Ar g Ar g L 210 215 lIi st car r ect ed. t xt igt gaa gt g gt t ggt gt t gt c tt c ~y O31u ValI Val G31y Val Val Phe 50 355 cc ggt t ac gt t ct c act gcc gaa hr G31y Ty r Val Leu Thr Al a O31u 370 .tc acc gcg ct g act cag cct gt c Ie Thr Al a Leu Thr O31n Pr o Val 385 agt cgt cgg gagct aggac cag 1171 1219 1267 1314 Il a 25 .eu ie r (l5 Il a 05 1le op~ op~ ~r o Il a 85 ~r o e u I Ie Al a ValI Al a Le u 75 li1e Ph e Le u Ar g Le u 1 55 Le u O31u ValI G31y Le u Thr Al a Ar g As n Ph e ValI Thr 125 Le u Ser Ser As n Hi s 205 G31y ValI Ser Al a Ph e Le u Ar g Le u 110 G31y G31y Lys5 Pr o ValI 1 ValI Ph e vb t Le u Al a Le u I Ie Ser Al a Le u Ph e I Ie Ph e 1 Thr vb t ValI Al a Le u Al a Le u G31y Ser Thr Pr a ValI Al a 1 Thr As n G31y Al a Ser Pro Asp Tyr Val Val Thr Asn Ala His Val Val Al a (3y Thr Ser 240 225 235 Page 79 seql i st cor r ect ed. Thr Val Ser Leu Asp Thr IVet I e G y Thr Arg Ser 245 250 Phe Tyr Asp Pro Asn Leu Asp lie Ala Val Leu Tyr 260 265 Sy Leu Asp Pro Leu Pro Trp Ala Ser Thr Pro Leu 275 280 Su Ala I e Val Met y Phe Pro n Ser y Pro 290 295 300 Pro Ala Arg Val Arg u Arg Ile IVet I e Thr y 305 310 315 Ala Asn G y G n His G u Arg G u Ala Tyr Ser Val 325 330 On Ser Gy Asn Ser Gy Gy Pro IVet Thr Asn u 340 345 Val y Val Val Phe G y Ala Ala Ile Asp y Ser 355 360 Val Leu Thr Ala Gu G u Val Gn G u Arg Ile G y 370 375 380 Leu Thr G n Pro Val Asp Thr Met G n Cys Ala Val 385 390 395 <210> 61 <211> 729 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(706) <223> RXA00566 <400> 61 cttgttgacc acgtgattac cttggctgaa ggcccaatca gca ctttaaagga gagaataaga ttatgagcag cggat t ccaa at g txt Al a Ser Asp 285 Phe Ser Ar g et Asp 365 Asp Ser Val 255 Asp Gy Al a SIle Ser 335 Gu Gy Thr Val Leu Asp Ser Tyr 320 I e Val Tyr Al a actaggc gcacggaaaa cca acg tcc cgt t ac Tyr acc Thr acc Thr gt c Val t ca Ser ct g Leu cct Pr o gt c Val gaa G u ggt Sy t cc Ser gca Al a gac Asp at g Met t cc Ser tt c Phe aaa Lys acc Thr gac Asp tt c Phe 75 at t SIle ct c Leu tct Ser cca Pr o acc Thr gag G u ttt Phe gca Al a 45 gac Asp gcg Al a cag G n gaa G u aac Asn cgc Ar g ttg Leu t cc gca Ser Al a 15 gag cgc G u Arg gac at c Asp Il e gat at c Asp Il e atg gca M\t Ala 80 Page IVet Pro Thr Ser Arg 1 tac ggc acc aaa gag Tyr y Thr Lys G u atc atc ttc ctg ggc Ile Ile Phe Leu G y atg gcg cag ctc ctt IVet Al a G n Leu Leu acc ctg tac atc aac Thr Leu Tyr Ile Asn att tac gac acc atg Ile Tyr Asp Thr Iet cag G n t cc Ser gtt Val acc Thr cgc Ar g 150 acc Thr gct Al a cgc Ar g gt c Val gcc Al a cct Pr o 120 ggt Sy cgt Ar g gag Su gaa G u ct c Leu 200 cca Pr o gtt Val t cc Ser gtt Val ttg Leu at c SIle 170 ct c Leu cgc Ar g seql i st cor r ect ed. t xt gac gtt cag acc gtc tgc ctt ggt Asp Val G n Thr Val Cys Leu y 95 ctt ctt gca gcc ggt gca cca ggt Leu Leu Ala Ala y Ala Pro G y 110 cgc gtg ctg atc cac cag cca gca Arg Val Leu Ile His G n Pro Ala 125 130 tct gac ctc gag atc cag gca gct Ser Asp Leu G u Ile G n Ala Ala 140 145 atg gaa acc acc ttg gct gag cac Mbt G u Thr Thr Leu Al a u Hi s 155 160 cgc atc gat acc gac cgt gac aag Arg lie Asp Thr Asp Arg Asp Lys 175 gag tat ggc atc gtt gac cag gtc Su Tyr Gy Ile Val Asp n Val 190 tagagttttt taaagattcg ggt cag G n aag Lys 115 acc Thr gaa G u acc Thr at c SIle ttc Phe 195 gca Al a 100 cgc Ar g cag G n at c SIle ggc G y ct c Leu 180 gat Asp gca Al a gct Al a ggc G y gag G u aag Lys 165 acc Thr t ac Tyr <210> 62 <211> 202 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 62 IVet Pro Thr Ser Arg Tyr Val Leu F 1 5 Tyr y Thr Lys G u Thr Asn Pro T Ile Ile Phe Leu y Thr n Val A 40 IVet Ala G n Leu Leu Val Leu G u C Thr Leu Tyr Ile Asn Ser Pro y C 70 lie Tyr Asp Thr IVet G n Tyr Val A Leu G y G n Al a Al a Ser Al a Al a A 100 1 Pro G y Lys Arg Ala Val Leu Pro A 115 120 Pro Ala Thr n y Thr n y C 130 135 Ala Ala u Ile u Arg IVet Arg A 'ro Ser Phe Ile u n Ser Ala yr 25 sp ay ay r g l a 05 sn Sn r g 10 Al a Lys Asp Thr IVet Asp Ser Phe 75 Pro Asp 90 Val Leu Ser Arg Val Ser Leu IVet Page 81 Phe Al a Asp Al a G n Al a Leu 125 Leu Thr G u Asn Ar g Leu Thr Al a 110 SIle G u Thr G u Asp Asp Met Val Sy Hi s SIle Leu Ar g SIe SIe Al a Cys Al a G n G n Al a seqI i st car r ect ed. t xt 145 150 155 160 O31u Hi s Th r O31y Lys Thr Ala Ou On lieArg lieAsp Thr Asp Ar g 165 170 175 As p Lys I Ie Leu Thr Ala 31u G31u Ala Leu Gu Tyr Gy Ilie Val As p 180 185 190 O31n Val Phe As p Ty r Ar g Lys Leu Lys Ar g 195 200 <210O> 63 <21 1> 714 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (691) <223> RXA00567 <400> 63 cgaacagagg cggt t tcat g gaaat accgc cgggt agt ct acgt acccaa gat t taagaa t gt aggagt t gact gtt t tc ggt gacat tg aaccaaat ga at g vb t ct c Le u at t I Ie cca Pr a ac c Thr at c I Ie ct t Le u at c I Ie at c I Ie c ag (31n 1 50 gcc A a agt Ser aac As n ag g Ar g ggc O31y ac c Thr ggt O31y at g vb t 120 at c I Ie at c I Ie ggt O31y cgc Ar g ct a Le u at c I Ie gca A a ggc G31y act Thr c ag O31n gct A a gag (31u cct Pr a at t I Ie gct A a ct g Le u t at Tyr G31y G31y t cc Ser c ag O31n 140 ac c Thr ggt O31y tt c Ph e c ag O31n 45 t ac Tyr g ac As p ct g Le u aag Lys5 gct A a 125 tt c Ph e ggc (31y tt c Ph e ct g Le u 30 at c I Ie at t I Ie ac c Thr gca A a cgt Ar g 110 ggt O31y gca A a c ag (31n gga O31y 15 gga O31y ct g Le u aac As n at g vb t gca A a 95 tt c Ph e gt t ValI gcc A a ac c Thr aat As n ag c Ser ct g Le u t cc Ser aaa Lys5 80 t cc Ser gca A a ggt O31y ac c Thr tt t Ph e 1 60 at g vb t 1 g ac As p c ag O31n ct g Le u cca Pr a t ac Tyr at g vb t tt g Le u ggt O31y aag Lys5 145 gag (31u agc gat at Ser Asp I I gt c ValI gt a ValI t cc Ser ggt O31y t cc Ser ggc O31y cca Pr a ac c Thr 130 cgt Ar g c ag O31n tt t Ph e g ac As p gct A a ggc O31y cca Pr a c ag O31n c ac Hi s 115 gca A a g aa O31u at c I Ie ga As 2 ga As ga 03 t c Se t g CY t t Ph 10 gc A gc A at t c Se t cgt e Ar g t cgc p Ar g 0 t gag p (1u g gat u As p c gt c r Val c gac s As p c ct g e Leu 0 a cgt a Ar g t gat a As p g gcc t A a c aag r Lys 1 Page 82 gat t cc gat cgt gac cgc t gg t tc a Asp Ser Asp Ar g As p Ar g Tr p Phe T 1 70 gga ctt gtt gac cac gt g at t acc t G31y Leu Val Asp Hi s Val I Ie Thr L 185 1 t aggcgcacg gaaaact t ta aag <210> 64 <211> 197 <21 2> PRTF <21 3> Cor ynebact er i urn gi ut anri curn lIi st car r ect ed. t xt ct gca cag gaa gct aag gat t ac hr Al a G n G u Al a Lys Asp Tyr 175 180 t g gct gaa ggc cca at c agc aac .eu Al a O31u 31y Pr o Ilie Ser Asn 90 195 <400> 64 I'bt Ser Asp I Ie As p O31n Le u Pr a Ty r vb t Le u G31y 145 O31u O31u G31y Ph e As p Al a G31y Pr a O31n Hi s 115 Al a Ou le Lys5 I Ie 1 Ar g IVbt Arg Leu O31u I Ie As p Pr o Val Thr 70 As p II e Leu Leu Ar g I Ie As p II e Al a 03 n 150 Lys As p 1 65 Ty r 03 y As n Al a vb t Al a Thr Al a Al a Ser vb t Al a 135 Le u Ser Le u O31n O31u Lys5 As p vb t Tyr G31y 1 Hi s O31n Al a Ar g As p 1 O31y 10 Ar g Le u I Ie Al a O31y 90 Th r O31n Al a O31u As p 1 70 Hi s Oy le cys Ser lIe 75 vb t Lys5 Pr a O31u Hi s 1 55 Ar g ValI Pr a I Ie Al a Le u Ty r O31y O31y Ser O31n 140 Thr Tr p I Ie O31y Ph e O31n Tyr As p Le u Lys5 Al a 125 Ph e O31y Ph e Th r O31y O31y Le u As n vb t Al a Ph e ValI Al a Thr Al a 1 Al a As n Ser Le u Ser Ly s Ser Al a O31y Thr Ph e 1 O31n aOu <210O> <211 <212> <213> <220> <221 <222> <223> 1 875 DNA Cor ynebact er i urn gi ut ani curn OIDS 101). (1852) RXN03 094 Page 83 seql i st cor r ect ed. t xt <400> atagatatta gagagttaaa taatggcgct tgacctgcag gaaattgaga tcaacactga ttgtgtaggt tggcgcccaa caaagaaagg gcgttgaaag atg agt tca ttc aat Met Ser Ser Phe Asn cca Pr o t cc Ser at c SIle ggt Gy tct Ser cgg Ar g ttg Leu cgc Ar g t at Tyr gt c Val 150 t cc Ser at t SIle cgt Ar g acc Thr cca Pr o 230 acc Thr gct Al a gag G u gat Asp ccc Pr o gcc Al a gat Asp aag Lys 120 gcc Al a act Thr gac Asp cgt Ar g aag Lys 200 at c SIle t cc Ser acc Thr aac Asn act Thr aag Lys gct Al a aat Asn t ac Tyr gat Asp aaa Lys gat Asp acc Thr 170 cag G n aac Asn gaa G u aag Lys aat Asn cct Pr o gat Asp gag G u tct Ser 75 gcg Al a gt c Val gct Al a gag G u cca Pr o 155 aag Lys gaa G u cct Pr o ggc Gy ggc G3y 235 gaa G u gat Asp ggc Gy at c I e ggc Gy ttc Phe t ca Ser gcg Al a gct Al a 140 gag G u ctt Leu at t SIle gtt Val ctt Leu 220 aaa Lys gcc Al a at t SIle gt a Val 45 ct c Leu gcc Al a act Thr acc Thr gat Asp 125 ttc Phe gga G y gct Al a cgt Ar g ct g Leu 205 gca Al a act Thr at g let cgt Ar g gca Al a gca Al a aat Asn gca Al a gaa G u 110 ct g Leu cct Pr o cag G n cgt Ar g cgc Ar g 190 at c SIle cgc Ar g ct g Leu cag G n 15 cca Pr o gcg Al a gag G u at g let gct Al a 95 gt a Val ttg Leu tcg Ser ttc Phe gaa G u 175 gt c Val ggt Gy cgc Ar g at c SIle gct Al a gct Al a cca Pr o gcc Al a gct Al a 80 cag G n ctt Leu acc Thr gtt Val cag G n 160 ggc Gy gtt Val gag G u at c SIle agt Ser 240 1 gct Al a cac Hi s gt c Val aag Lys aat Asn gag G u ctt Leu aac Asn cgt Ar g 145 gct Al a aag Lys cag G n cca Pr o gtt Val 225 ctt Leu ctt Leu ct g Leu ct c Leu aag Lys cca Pr o ctt Leu gcc Al a aag Lys 130 gga G y ttg Leu at t SIle gt g Val ggt G y 210 gct Al a gat Asp cag G n gct Al a gct Al a gtt Val ttc Phe ggt G y 100 at c l Ie gca Al a cag G n aag Lys cct Pr o 180 agc Ser ggt G y gac Asp ggt G y gca Al a gcc Al a act Thr gct Al a aac Asn gag G u gct Al a acc Thr cgt Ar g t ac Tyr 165 gtt Val cgt Ar g aaa Lys gtt Val t cc Ser 245 atg gtt gcc ggc gct aag tat cgc ggt gaa ttc gag gag cga ctg aag Page 84 s lIVt Val Ala Gy Ala Lys Tyr Arg 250 eql i st cor r ect ed. t xt G y u Phe Gu G u Arg Leu Lys 260 gct Al a at c SIle gcc Al a ct g Leu 310 gaa G u gag G u cgc Ar g gcc Al a gat Asp 390 gag G u cgc Ar g gct Al a cgc Ar g gct Al a 470 tct Ser aag Lys ct g Leu gag G u 280 gat Asp ttg Leu gac Asp acg Thr gag G u 360 gct Al a gct Al a gat Asp ct c Leu aag Lys 440 aag Lys gac Asp tcg Ser cgc Ar g gag G u cac Hi s gga G y ggt G y gcc Al a 330 gaa G u cat Hi s ct c Leu gac Asp t ca Ser 410 at c SIle cgt Ar g tct Ser gt c Val att SIle 490 ggc G y at c SIle acc Thr aac Asn gcc Al a 315 ct g Leu gat Asp cac Hi s t ca Ser tta Leu 395 cct Pr o gaa G u ct a Leu gag G u cgg Ar g 475 gca Al a cga Ar g aag Lys at c SIle at g let 300 acc Thr gag G u gcc Al a ggt G y aac Asn 380 gtt Val cag G n gag G u gaa G u ttg 460 gag G u aaa Lys at c SIle gga G y gt c Val 285 att SIle acg Thr cgt Ar g at c l Ie gt c Val 365 cgc Ar g gat Asp gaa G u at g let aag Lys 445 aag Lys at g let cgt Ar g cct Pr o gct Al a 270 ggc G y aag Lys ct g Leu agg Ar g ggt G y 350 cgc Ar g t at Tyr gag G u at c lile gcg Al a 430 ct g Leu gct Al a aaa Lys gac Asp gag 10u 510 255 aac Asn gct Al a cca Pr o aat Asn ttc Phe 335 at t SIle at c SIle at c SIle gca Al a gat Asp 415 ct g Leu cgc Ar g cgt Ar g gaa G u ggc G y 495 ct g Leu ggc G y ggt G y ct g Leu gag G u 320 cag G n ctt Leu cag G n acc Thr gca Al a 400 gag G u t cc Ser tcg Ser tgg Tr p gag G u 480 aat Asn gaa G u gaa G u gct Al a ctt Leu 305 t ac Tyr cag G n cgt Ar g gac Asp agc Ser 385 t ca Ser ct g Leu aag Lys gaa G u cag G n 465 ct g Leu t at Tyr aag Lys gt c Val tcg Ser 290 gcc Al a cgc Ar g gtt Val gga G y t cc Ser 370 cgt Ar g cgc Ar g gag G u gaa G u ctt Leu 450 aat Asn gaa G u tgt Cys cag G n acc Thr gaa G u ggt G y t ac Tyr gt c Val 340 aag Lys ct g Leu ctt Leu cgc Ar g at c SIle 420 gat Asp gat Asp aaa Lys ct g Leu gt c Val 500 gag G u ttc Phe t cc Ser gag G u at c SIe 325 ggt G y gaa G u gt c Val cct Pr o at g Met 405 gt c Val gca Al a gaa G u act Thr cgt Ar g 485 gca Al a gat Asp 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 gca gaa tcc aag gtc gag gtc aat gaa aat gcc atg ctc act gag gag Page Al a gt c ValI cct Pr o 550 gag (31u t gc Cys Ser 520 cca Pr o G31y gt c ValI gt c ValI seqI i st car r ect ed. t xt Lys Val O31u Val As n O31u As n Al a I'b t Leu Thr O31u O31u 525 530 gac acg at c gcc gat gtg9 gt t t cc gca t gg acg ggc at t As p Thr I Ie Al a As p Val Val Ser Al a Tr p Thr O31y I Ie 540 545 aag atg at g cag ggt gag acc gag aag ctg ctc aac atg Lys I'b t 'b t O31n 31y O31u Thr O31u Lys Leu Leu Asn I'b t 555 560 565 t tg ggc aac cgt gt g gt c ggt cag ct a gaa agc ggt aac Leu (1y Asn Ar gVal Val G31y (1n Leu (1u Ser 31y Asn 570 575 580 t gacgcggt g cgt cgt tcac gcg 1 747 1 795 1 843 1 875 <210O> 66 <21 1> 584 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 66 I'bt Ser Ser Al a Leu 03 n Hi s Leu Leu Val Leu I'b t Lys Lys Leu Asn Pro Asn 03 u Leu Al a Leu Al a 03 y 115 Asn Lys 03 y 130 Ar g O31y Ser 145 Al a Leu 03 u Lys Il e Asp O31n Val Leu 1 95 Phe Asn Pro Thr 03 n Al a Se r Se r Al a Al a I I e Leu Al a Thr 03 y Val 55 Val Al a Ser Ty r 70 Phe As n Ar g As p O3 y 03 u Leu 03 y 1 00 I Ie Al a Ar g O31y Al a Thr Ty r As p 135 O31n Ar g Val Thr 150 Lys Ty r Ser Thr 1 65 Pr o Val I Ie O31y 1 80 Ser Ar g Ar g Thr Thr Lys Thr 10 Al a 03 y As n 25 03 u 03 n Th r 40 Asp Pro Lys Pr o Lys Al a Al a Leu Asn 90 Asp 03 u Tyr 1 05 Lys Ser Asp 120 Al a IlIe Lys Thr 03 n Asp Asp Leu Thr 1 70 Ar g Asp O31n 1 85 Lys Asn Asn 200 As n Pr o As p O31u Ser 75 Al a ValI Al a O31u Pr a 1 55 Ly s O31u Pr o O31u As p O31y I Ie O31y Ph e Ser Al a Al a 140 O31u Le u I Ie ValI Al a I Ie ValI Le u Al a Thr Thr As p 125 Ph e O31y Al a Ar g Le u 205 vb t Ar g Al a Al a As n Al a O31u 110 Le u Pr a O31n Ar g Ar g 1 li1e O31n Pr a Al a O31u vb t Al a ValI Le u Ser Ph e O31u 1 ValI G31y Al a Al a Pr a Al a Al a O31n Le u Thr ValI O31n 1 G31y ValI O31u Pro G31y 210 Val Gy Lys Thr Ala IlieVal Ou Gy Leu Ala Arg Arg Ilie 215 220 Page 86 seqi i st car r ect ed. t xt Val Ala G1y Asp Val Pro Ou Se 225 230 r Leu Lys Le u O31u O31u A a Le u 305 Tyr O31n Ar g As p Ser 385 Ser Le u Ly s O31u O31n 465 Le u Tyr Ly s vb t A a 545 Ly s Le u Ar g ValI 275 O31y Ar g Ly s Tyr Le u 355 A a Ph e Le u Ar g Ser 435 A a O31u A a Ar g I Ie 515 Thr Thr Le u Ser 245 Ly s Ph e Ser O31u I Ie 325 O31y O31u ValI Pr o vb t 405 ValI A a O31u Thr Ar g 485 A a As p O31u I Ie vb t 565 vb t A a I Ie A a Le u 310 O31u O31u Ar g A a As p 390 O31u Ar g A a Ar g A a 470 Ser Ly s A a ValI Pr o 550 O31u ValI ValI As p vb t 295 Ar g Ly s Pr o Tyr A a 375 Ly s I Ie Ar g Ser O31u 455 li1e O31u Le u O31u Thr 535 A a Ar g A a Le u O31u 280 As p Le u As p Thr O31u 360 A a A a As p Le u Ly s 440 Ly s As p Ser Ar g Ser 520 Pr o O31y ValI O31y As p 265 Le u A a ValI A a ValI 345 ValI O31u I Ie Ser O31u 425 O31u Le u As p As p Tyr 505 Ly s As p Ly s Le u (31y 235 Ly s I Ie Thr As n A a 315 Le u As p Hi s Ser Le u 395 Pr a O31u Le u O31u Ar g 475 A a Ar g O31u I Ie vb t 555 As n Lys Thr Tyr Ly s I Ie vb t 300 Thr O31u A a G31y As n 380 ValI O31n (31u (31u Le u 460 (31u Ly s I Ie ValI A a 540 O31n Ar g Leu I I e Ser Ar g O31y ValI 285 li1e Thr Ar g I Ie ValI 365 Ar g As p O31u vb t Ly s 445 Ly s vb t Ar g Pr a As n 525 As p O31y ValI O31y A a 270 O31y Ly s Le u Ar g O31y 350 Ar g Tyr O31u I Ie A a 430 Le u A a Ly s As p O31u 510 O31u ValI O31u ValI O31u 255 As n A a Pr a As n Ph e 335 li1e I Ie I Ie A a As p 415 Le u Ar g Ar g O31u O31y 495 Le u As n ValI Thr O31y 575 240 Ph e O31y O31y Le u O31u 320 O31n Le u O31n Thr A a 400 O31u Ser Ser Tr p O31u 480 As n O31u A a Se r O31u 560 O31n Leu 03 u Ser Asn Cys Ser Val Page 87 seqi i st car r ect ed. t xt <210O> 67 <21 1> 1816 <21 2> DNA <21 3> COr ynebact er i urn gi ut arr cumn <220> <221 CDS <222> (1816) <223> FRXA01668 <400> 67 at agat at ta gagagt taaa t aat ggcgct t gacct gcag ttgtgtaggt tggcgcccaa caaagaaagg gcgttgaaag gaaat tgaga t caacact ga at g agt t ca t tc aat 11! l'bt Ser Ser Phe Asn cca Pr o t cc Ser at c I Ie ggt G31y t ct Ser cgg Ar g tt g Le u cgc Ar g t at Tyr gt c ValI 1 50 t cc Ser at t I Ie ac c Thr gct A a gag O31u g at As p ccc Pr o gcc A a g at As p aag Ly s 120 gcc A a act Thr g ac As p cgt Ar g ac c Thr aac As n act Thr aag Ly s gct A a aat As n t ac Tyr g at As p aaa Ly s g at As p ac c Thr 1 70 c ag O31n aat As n cct Pr o g at As p gag O31u t ct Ser 75 gcg A a gt c ValI gct A a gag O31u cca Pr o 155 aag Ly s gaa O31u g aa O31u g at As p 99c G31y at c lIe 99c G31y Ph e t ca Ser gcg A a gct A a 140 gag O31u ct t Le u at t I Ie gcc A a at t I Ie gt a ValI 45 ct c Le u gcc A a act Thr ac c Thr g at As p 125 tt c Ph e gga G31y gct A a cgt Ar g at g vb t cgt Ar g gca A a gca A a aat As n gca A a g aa O31u 110 ct g Le u cct Pr a c ag O31n cgt Ar g cgc Ar g 1 c ag O31n 15 cca Pr a gcg A a gag O31u at g vb t gct A a 95 gt a ValI tt g Le u t cg Ser tt c Ph e g aa (31u 1 75 gt c ValI gct A a gct A a cca Pr a gcc A a gct A a 80 c ag O31n ct t Le u ac c Thr gt t ValI c ag O31n 1 60 ggc O31y gt t ValI 1 gct A a c ac Hi s gt c ValI aag Ly s 65 aat As n gag O31u ct t Le u aac As n cgt Ar g 145 gct A a aag Ly s c ag O31n ct t Le u ct g Le u ct c Le u 50 aag Ly s cca Pr a ct t Le u gcc A a aag Ly s 130 gga O31y tt g Le u at t I Ie gt g ValI c ag O31n 20 gct A a gct A a gt t ValI tt c Ph e ggt O31y 1 00 at c I Ie gca A a c ag O31n aag Ly s cct Pr a 1 ag c Ser gca A a gcc A a act Thr gct A a aac As n gag O31u gct A a ac c Thr cgt Ar g t ac Tyr 1 gt t ValI cgt Ar g cgt acc aag aac aac cct gt t ct g at c ggt gag cca ggt gt c ggt aaa Page 88 Ar g Thr Lys 200 Asn Asn Pro Val Leu 205 eqi i st car r ect ed. t xt le 31y O31u Pr o G31y 210 Val G31y Lys ac c Thr cca Pr o 230 at g vb t gct A a at c I Ie gcc A a ct g Le u 310 g aa O31u gag O31u cgc Ar g gcc A a g at As p 390 gag O31u cgc Ar g gct A a cgc Ar g at c I Ie t cc Ser gcc A a ct g Le u gag O31u 280 g at As p tt g Le u g ac As p ac g Thr gag O31u 360 gct A a gct A a g at As p ct c Le u aag Ly s 440 aag Ly s g aa O31u aag Ly s gct A a 250 gag O31u c ac Hi s gga G31y ggt G31y gcc A a 330 g aa O31u cat Hi s ct c Le u g ac As p t ca Ser 410 at c I Ie cgt Ar g t ct Ser ggc G31y G31y 235 aag Ly s at c I Ie acc Thr aac As n gcc A a 315 ct g Le u g at As p cac Hi s t ca Ser t t a Le u 395 cct Pr o gaa O31u ct a Le u gag (31u ct t Le u 220 aaa Ly s t at Tyr aag Ly s at c I Ie at g vb t 300 ac c Thr gag O31u gcc A a ggt G31y aac As n 380 gt t ValI c ag O31n gag O31u g aa O31u tt g Le u 460 gca A a act Thr cgc Ar g gga G31y gt c ValI 285 at t I Ie ac g Thr cgt Ar g at c I Ie gt c ValI 365 cgc Ar g g at As p g aa O31u at g vb t aag Ly s 445 aag Ly s cgc Ar g ct g Le u ggt G31y gct A a 270 ggc G31y aag Ly s ct g Le u ag g Ar g ggt G31y 350 cgc Ar g t at Tyr gag O31u at c I Ie gcg A a 430 ct g Le u gct A a cgc Ar g at c I Ie g aa O31u 255 aac As n gct A a cca Pr o aat As n tt c Ph e 335 at t I Ie at c I Ie at c I Ie gca A a g at As p 415 ct g Le u cgc Ar g cgt Ar g at c I Ie agt Ser 240 tt c Ph e ggc G31y ggt G31y ct g Le u gag O31u 320 c ag O31n ct t Le u c ag O31n ac c Thr gca A a 400 gag O31u t cc Ser t cg Ser t gg Tr p gt t ValI 225 ct t Le u gag O31u g aa O31u gct A a ct t Le u 305 t ac Tyr c ag O31n cgt Ar g g ac As p ag c Ser 385 t ca Ser ct g Le u aag Ly s g aa O31u c ag O31n 465 gct A a g at As p gag O31u gt c ValI t cg Ser 290 gcc A a cgc Ar g gt t ValI gga O31y t cc Ser 370 cgt Ar g cgc Ar g gag O31u g aa O31u ct t Le u 450 aat As n ggt O31y ct t Le u cga Ar g gt t ValI 275 ggt O31y cgc Ar g aag Ly s t at Tyr tt g Le u 355 gca A a tt c Ph e ct g Le u cgt Ar g t cc Ser 435 gct A a gag O31u g ac As p ggt O31y ct g Le u 260 ac c Thr g aa O31u ggt O31y t ac Tyr gt c ValI 340 aag Ly s ct g Le u ct t Le u cgc Ar g at c I Ie 420 g at As p g at As p aaa Ly s gt t ValI t cc Ser 245 aag Ly s tt c Ph e t cc Ser gag O31u at c I Ie 325 ggt O31y g aa O31u gt c ValI cct Pr o at g vb t 405 gt c ValI gca A a g aa O31u act Thr 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 1315 1363 1411 1459 1 507 1 555 gct at t gac gat gt c cgg gag at g aaa gaa gag ct g gaa gcg ct g cgt Page 89 Al a 470 tct Ser aag Lys gca Al a gt c Val cct Pr o 550 gag G u Asp tcg Ser cgc Ar g t cc Ser 520 cca Pr o ggc G y gt c Val Val at t SIle 490 ggc G y gt c Val acg Thr at g Met ggc G0y 570 Ar g 475 gca Al a cga Ar g gag G u at c SIle at g Met 555 aac Asn G u aaa Lys at c SIle gt c Val gcc Al a 540 cag G n ccg Pr o seql i st cor r ect ed. t xt IVet Lys G u G u Leu G u 480 cgt gac ggc aat tat tgt Arg Asp Gy Asn Tyr Cys 495 cct gag ctg gaa aag cag Pro u Leu u Lys n 510 aat gaa aat gcc atg ctc Asn G u Asn Ala IVet Leu 525 530 gat gtg gtt tcc gca tgg Asp Val Val Ser Ala Trp 545 ggt gag acc gag aag ctg G y G u Thr G u Lys Leu 560 Al a cgt Ar g at c SIle 515 act Thr acg Thr ct c Leu Leu gt c Val 500 gag G u gag G u ggc Gy aac Asn Ar g 485 gca Al a gat Asp gag G u at t lle at g Met 565 1603 1651 1699 1747 1795 1816 <210> 68 <211> 572 <212> PRT <213> Corynebact <400> 68 IVet Ser Ser Phe 1 Al a Leu G n G n His Leu Leu Ala Val Leu bet Al a Lys Lys Leu Val Asn Pro Asn Phe G u Leu Al a G y 100 Leu Al a G y Ile 115 Asn Lys G y Al a 130 Arg y Ser G n 145 Ala Leu G u Lys er i um gl ut am cum Asn Pro Thr Thr Lys Thr 5 10 Al a Ser Ser Al a y Asn 25 Ala lie Leu G u G n Thr 40 Thr G y Val Asp Pro Lys 55 Ala Ser Tyr Pro Lys Ala 70 Asn Arg Asp Ala Leu Asn 90 G u Leu G y Asp G u Tyr 105 Ala Arg y Lys Ser Asp 120 Thr Tyr Asp Ala lle Lys 135 Arg Val Thr Thr G n Asp 150 Tyr Ser Thr Asp Leu Thr 165 170 Asn Pr o Asp G u Ser Al a Val Al a G u Pr o 155 Lys G u Asp Gy SIle Gy Phe Ser Al a Al a 140 G u Leu Al a SIle Val Leu Al a Thr Thr Asp 125 Phe Gy Al a vet Ar g Al a Al a Asn Al a G u 110 Leu Pr o G n Ar g G n Pr o Al a G u et Al a Val Leu Ser Phe G u 175 Lys Ile Asp Pro Val Ile Gy Arg Asp On Ou Ile Arg Arg Val Val Page seql i st cor r ect ed. t xt 185 Asn Asn Pro Val G n Pr o Val 225 Leu G u G u Al a Leu 305 Tyr G n Ar g Asp Ser 385 Ser Leu Lys G u G n 465 Leu Tyr Lys Met Leu 195 Val Gy Leu Ar g Val 275 Gy Ar g Lys Tyr Leu 355 Al a Phe Leu Ar g Ser 435 Al a G u Al a Ar g SIle 515 Thr Ar g Lys Val Ser 245 Lys Phe Ser G u SIle 325 Gy G u Val Pr o bet 405 Val Al a G u Thr Ar g 485 Al a Asp G u Arg Thr Thr Al a 215 Pro G u 230 vet Val Al a Val Ile Asp Al a Vbet 295 Leu Arg 310 G u Lys G u Pro Arg Tyr Al a Al a 375 Asp Lys 390 G u lie Arg Arg Al a Ser Arg G u 455 Ala I e 470 Ser G u Lys Leu Al a u Val Thr 535 Lys 200 l le Ser Al a Leu G u 280 Asp Leu Asp Thr G u 360 Al a Al a Asp Leu Lys 440 Lys Asp Ser Ar g Ser 520 Pr o Val Leu Gy Asp 265 Leu Al a Val Al a Val 345 Val G u l Ie Ser G u 425 G u Leu Asp Asp Tyr 505 Lys Asp Gy Gy 235 Lys SIle Thr Asn Al a 315 Leu Asp Hi s Ser Leu 395 Pr o G u Leu G u Ar g 475 Al a Ar g G u lie Leu 205 Al a Thr Ar g Gy Val 285 I le Thr Ar g SIle Val 365 Ar g Asp G u et Lys 445 Lys Met Ar g Pr o Asn 525 Asp 190 lie G y u Arg Arg Il e Leu Il e Ser 240 G y G u Phe 255 Al a Asn G y 270 G y Al a G y Lys Pro Leu Leu Asn G u 320 Arg Phe G n 335 G y Ile Leu 350 Arg lie n Tyr Ile Thr G u Ala Ala 400 Ile Asp G u 415 Ala Leu Ser 430 Leu Arg Ser Al a Ar g Tr p Lys G u G u 480 Asp G y Asn 495 G u Leu G u 510 G u Asn Ala Val Val Ser Page 91 seqI i st car r ect ed. t xt AlaTrpThr Oy Ilie Pro Ala(3y Lys I'bt I'bt On Oy Ou Thr Ou 545 550 555 560 Lys Leu Leu Asn I'bt O31u Ar g Val Leu G31y Asn Pr o 565 570 <210O> 69 <21 1> 1401 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1378) <223> RXN01120 <400> 69 acaggt aaag cgct aagat g gaacaaccca t tgccaat at agt aaat ct t t t caat cgt g gaagcgggt c t cacagt ct a t gt tggt tag agt tgt acgc at g gca cgt at g cag 11! I'bt Ala Ar g I'bt O31n g aa G u c ag 0On t gc CYS c aa 0On t ca Ser at c I Ie g aa 0Ou aag Lys ctt Le u g at As p 1 50 aac As n gcc A a aaa Ly s gag O31u g ac As p tt c Ph e t cg Ser at c I Ie aat As n 120 c ag O31n ac c Thr tt g Le u ct g Le u ct c Le u t gc cys c ag O31n g at As p gcg A a cgt Ar g tt g Le u tt g Le u ct g Le u aag Ly s 1 70 ct c Le u at c I Ie aac As n gag O31u act Thr 75 gt g ValI cgc Ar g at g vb t gca A a acc Thr 155 ct g Le u aaa Ly s gcg A a gag O31u cgc Ar g t at Tyr t ac Tyr ag g Ar g ct c Le u aag Ly s 140 gag O31u ct t Le u t gt cys ggt O31y at t lIe 45 aac As n gt c ValI aac As n aat As n ggc O31y 125 ct g Le u gct A a c ag O31n t cc Ser ggc O31y at t I Ie gag O31u at c I Ie cat Hi s g ac As p 110 ccc Pr a ct g Le u ggt O31y gct A a tt c Ph e 15 gcc A a g aa O31u ct c Le u ggg O31y t ac Tyr 95 gag O31u act Thr g at As p t at Tyr gct A a 1 75 t gc cys gt a ValI g aa O31u ccc Pr a c ag O31n 80 aag Ly s cct Pr a O31y gt t ValI gt g ValI 1 60 g at As p 1 gga G y t at Tyr g aa 0Ou aag Ly s 65 g ac As p cgt Ar g g aa 0Ou t cc Ser cct Pr a 145 ggc 0Oy Ph e aag Ly s at c I Ie ct c Le u 50 ccg Pr a cca Pr a ct c Le u ac c Thr ggc O31y 130 tt t Ph e gag O31u g at As p ag c Ser t gt cys 35 ggt O31y t cg Ser gca A a cgc Ar g g aa O31u 115 aag Ly s gct A a g at As p gt g ValI c aa G n 20 g at As p c aa O n gag 0Ou aaa Ly s gca A a 1 00 ct g Le u act Thr at c I Ie gt g ValI g aa 0Ou 1 aag Ly s gag O31u gct A a at t I Ie cgt Ar g t cg Ser gt t ValI tt c Ph e gcg A a g aa O31u 1 cgt Ar g Page 92 gca cag cgc ggc Ala n Arg G y 185 atc att tac atc Ile lie Tyr Ile eql i st cor r ect ed. t gat gaa gtg gac Asp G u Val Asp 190 aag Lys cag G n ccg Pr o 230 acc Thr aag Lys gag Gu gat Asp at t SIle 310 tct Ser t at Tyr gat Asp gcc Al a t at Tyr 390 gcc Al a gaa Gu gaa G u 200 gca Al a gga Gy aac Asn at c SIle tct Ser 280 ct c Leu cgt Ar g gt c Val cgt Ar g ttg Leu 360 ggc Gy ct c Leu gcc Al a aag Lys cca Pr o ct g Leu cgc Ar g ttg Leu 250 gac Asp aag Lys gag G u cca Pr o gtt Val 330 ttt Phe gag G u cgc Ar g gac Asp ggc Gy 410 tcg Ser aaa Lys aag Lys 235 tt c Phe cgc Ar g aag Lys gac Asp gt c Val 315 ct c Leu gaa G u at c SIle gcg Al a cgt Ar g 395 gaa G u at c SIle at t SIle 220 cac Hi s at c SIle aat Asn gaa Gu ct g Leu 300 gtt Val acg Thr at g Met gct Al a at c l Ie 380 aaa Lys gcc Al a act Thr 205 ttg Leu ccc Pr o gtt Val ggc Gy gaa Gu 285 gt g Val gcc Al a gag G u gat Asp aat Asn 365 at g Met gac Asp gaa G u cgc gat Arg Asp gaa ggc G u Gy aac cag Asn n gct ggt Al a G y 255 aag aaa Lys Lys 270 gcc aac Al a Asn aag ttt Lys Phe acc gt a Thr Val cct cgt Pro Arg 335 gac gct Asp Al a 350 cag gca G n Al a gaa gag G u G u gtt ggc Val G y cca gag Pro G u 415 gtt Val act Thr gat Asp 240 gcg Al a ggc Gy at t SIle ggt Gy t cc Ser 320 aac Asn gt g Val ct c Leu at c SIle gaa G u 400 at g Met t cc Ser gt c Val 225 ttc Phe ttc Phe ttg Leu gt g Val ct c Leu 305 aac Asn t ca Ser ttg Leu gag G u ct g Leu 385 gt c Val ttg Leu aag att tcc cgc Lys Ile Ser Arg 195 ggt gaa ggc gtg G y G u G y Val 210 gcc gca atc cca Ala Ala lie Pro atc cag ctg gat Ile G n Leu Asp 245 tct ggt ctg gag Ser G y Leu G u 260 ggc ttc ggt gtg G y Phe G y Val 275 gat atc ttc aag Asp Ile Phe Lys 290 atc cca gaa ttc lie Pro u Phe ctg gat cag aaa Leu Asp G n Lys 325 ttg gtg aag cag Leu Val Lys G n 340 acc ttt act gat Thr Phe Thr Asp 355 cgc aaa act ggc Arg Lys Thr y 370 gtt ccg atc atg Val Pro lle bet atc atc aac ggt Ile Ile Asn G y 405 gaa gct gtc gca G u Ala Val Ala 420 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1401 acc gcg Thr Al a 425 tagttggcag gagttatcac cgg <210> <211> 426 <212> PRT Page 93 seqI i st car r ect ed. t xt <21 3> Cor ynebact er i urn gi ut ani cumn <400> I'b t Al a Ar g 'b t O31n 03 u Se r G31y Ty r O31u Ly s As p Ar g O31u Ser Pr a 145 G31y Ph e As p Ser ValI 225 Ph e Ph e Le u ValI Le u 305 As n Ser cys G31y Ser Al a Ar g O31u 115 Ly s Al a As p ValI I Ie 1 95 O31u Al a O31n G31y Ph e 275 li1e Pr a As p Ly s O31u Al a I Ie Ar g Ser ValI Ph e Al a O31u 1 65 Ar g Ar g ValI Pr a As p 245 O31u ValI Ly s Ph e Ly s 325 O31n Cy s O31n Ser 70 li1e O31u Ly s Le u As p 150 As n Al a Ly s O31n Pr a 230 Th r Ly s O31u As p I Ie 310 Ser ValI I Ie Hi s Al a Le u Th r Ser Al a 135 Al a I Ie O31n Ser O31n 215 O31n Th r ValI ValI ValI 295 O31y Le u Al a Asp Leu 10 Lys Lys Leu O3 u Leu Cys 40 Asp O31u O31n Phe Leu Asp Ser Val Al a 90 Ilie O31y Ar g 1 05 Asn Il e Leu 120 O3 n Thr Leu Thr Ser Leu Leu Leu Lys 1 70 Ar g O31y Ilie 1 85 O31u Asn Pr o 200 Al a Leu Leu O31y O31y Ar g Asn Il e Leu 250 Il e Al a Asp 265 Ser Ser Lys 280 Leu Pr o O31u Ar g Leu Pr o Val Lys Val 330 Le u I Ie As n O31u Thr ValI Ar g vb t Al a Thr 1 Le u I Ie Ser Ly s Ly s 235 Ph e Ar g Ly s As p ValI 315 Le u Ly s Al a O31u Ar g Tyr Tyr Ar g Le u Ly s 140 O31u Le u Tyr I Ie I Ie 220 Hi s I Ie As n O31u Le u 300 ValI Thr Cys O31y lIe As n ValI As n As n O31y 125 Le u Al a O31n I Ie Thr 205 Le u Pr a ValI O31y O31u 285 ValI Al a O31u Ser O31y li1e O31u I Ie Hi s As p 110 Pr a Le u O31y Al a As p 1 Ar g O31u As n Al a Ly s 270 Al a Ly s Thr Pr a Ph e Al a O31u Le u O31y Tyr O31u Thr As p Tyr Al a 1 O31u As p O31y O31n O31y 255 Ly s As n Ph e ValI Ar g 335 Ser Leu Val On Tyr Arg Arg Leu Phe Ou N/t Asp 345 Page 94 Asp Al a Val 350 Leu Thr G u Ar g 370 Leu Val 385 Val lile Leu G u seql i st cor r ect ed. t xt Phe Thr Asp Asp Ala Leu G u G u Ile Ala Asn G n Ala Leu 355 360 365 Lys Thr y Ala Arg y Leu Arg Ala lie IVet u u lie 375 380 Pro lie IVet Tyr Asp Leu Pro Asp Arg Lys Asp Val y G u 390 395 400 Ile Asn G y Ala Val Al a Arg y u Al a u Pro u IVet 405 410 415 Ala Val Ala G u G u Lys Thr Ala 420 425 <210> 71 <211> 1401 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1378) <223> FRXA01120 <400> 71 acaggtaaag cgctaagatg gaacaaccca ttgccaatat agtaaatctt ttcaatcgtg gaagcgggtc tcacagtct a tgttggttag agttgtacgc gaa G u cag G n tgc Cys caa G n t ca Ser at c l Ie gaa G u aag Lys gcc Al a aaa Lys gag Gu gac Asp ttc Phe tcg Ser at c SIle aat Asn 120 ct g Leu ct c Leu tgc Cys cag G n gat Asp gcg Al a cgt Ar g ttg Leu aaa Lys gcg Al a gag Gu cgc Ar g t at Tyr t ac Tyr agg Ar g ct c Leu t cc Ser ggc G y at t SIle gag G u at c SIle cat Hi s gac Asp 110 ccc Pr o ttc Phe 15 gcc Al a gaa Gu ct c Leu ggg Sy t ac Tyr 95 gag G u act Thr tgc Cys gt a Val gaa Gu ccc Pr o cag G n aag Lys cct Pr o ggc Sy at g Met 1 gga G y t at Tyr gaa Gu aag Lys gac Asp cgt Ar g gaa G u t cc Ser aag Lys at c SIle ct c Leu ccg Pr o cca Pr o ct c Leu acc Thr ggc Sy 130 gca cgt at g Al a Arg IVet agc Ser tgt Cys ggt Gy tcg Ser gca Al a cgc Ar g gaa G u 115 aag Lys caa G n gat Asp caa Gn gag G u aaa Lys gca Al a 100 ctg Leu act Thr ctt gcc cag act ttg gca aag ctg ctg gat gtt Leu Ala G n Thr Leu Al a Lys Leu Leu Asp Val cct ttt gct atc gcg Pro Phe Ala lie Ala Page seql i st cor r ect ed. t xt gat Asp 150 aac Asn gca Al a aag Lys cag G n ccg Pr o 230 acc Thr aag Lys gag G u gat Asp at t SIle 310 tct Ser t at Tyr gat Asp gcc Al a t at Tyr 390 acc Thr ttg Leu cgc Ar g gaa G u 200 gca Al a gga Gy aac Asn at c SIle tct Ser 280 ct c Leu cgt Ar g gt c Val cgt Ar g ttg Leu 360 ggc Gy ct c Leu ct g Leu aag Lys 170 at c SIle cca Pr o ct g Leu cgc Ar g ttg Leu 250 gac Asp aag Lys gag G u cca Pr o gtt Val 330 ttt Phe gag G u cgc Ar g gac Asp acc Thr 155 ct g Leu at t SIle tcg Ser aaa Lys aag Lys 235 tt c Phe cgc Ar g aag Lys gac Asp gt c Val 315 ct c Leu gaa G u at c SIle gcg Al a cgt Ar g 395 gag G u ctt Leu t ac Tyr at c SIle at t SIle 220 cac Hi s at c SIle aat Asn gaa G u ct g Leu 300 gtt Val acg Thr at g lbt gct Al a at c l Ie 380 aaa Lys gct Al a cag G n at c SIle act Thr 205 ttg Leu ccc Pr o gtt Val ggc G y gaa G u 285 gt g Val gcc Al a gag G u gat Asp aat Asn 365 at g lbt gac Asp ggt G y gct Al a gat Asp 190 cgc Ar g gaa G u aac Asn gct Al a aag Lys 270 gcc Al a aag Lys acc Thr cct Pr o gac Asp 350 cag G n gaa G u gtt Val t at Tyr gct Al a 175 gaa G u gat Asp ggc Gy cag G n ggt G y 255 aaa Lys aac Asn ttt Phe gt a Val cgt Ar g 335 gct Al a gca Al a gag G u ggc Gy gt g Val 160 gat Asp gt g Val gtt Val act Thr gat Asp 240 gcg Al a ggc G y at t SIle ggt G y t cc Ser 320 aac Asn gt g Val ct c Leu at c SIle gaa G u 400 gag G u gat Asp aag Lys ggt Sy 210 gcc Al a at c SIle tct Ser ggc G y gat Asp 290 at c SIle ct g Leu ttg Leu acc Thr cgc Ar g 370 gtt Val at c SIle gat Asp gt g Val at t SIle 195 gaa G u gca Al a cag G n ggt G y ttc Phe 275 at c SIle cca Pr o gat Asp gt g Val ttt Phe 355 aaa Lys ccg Pr o at c SIle gt g Val gaa G u 180 t cc Ser ggc Sy at c l Ie ct g Leu ct g Leu 260 ggt G y ttc Phe gaa G u cag G n aag Lys 340 act Thr act Thr at c SIle aac Asn gaa G u 165 cgt Ar g cgc Ar g gt g Val cca Pr o gat Asp 245 gag G u gt g Val aag Lys ttc Phe aaa Lys 325 cag G n gat Asp ggc Sy at g Met ggt Sy 405 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 gcc gtt gcc cgt ggc gaa gcc gaa cca gag atg Ala Val Ala Arg Oy u Ala u Pro u IVet ttg gaa gct gtc gca Leu u Ala Val Ala Page 96 seqI i st car r ect ed. t xt 410 415 gaa gaa aag acc gcg tagttggcag gagttatcac cgg G u Gu Lys Thr Al a 425 1401 <210O> 72 <21 1> 426 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 72 vb t G31y Ty r (31u Ly s As p Ar g (31u Ser Pr o 145 G31y Ph e As p Ser ValI 225 Ph e Ph e Le u Al a Ar g 'b t G31n G3 u Se r Lys Ser a3 n 1i1 e Cys Asp Leu a3 y a3 n Pr o Ser G u Pr o Al a Lys Leu Ar g Al a 1 00 Thr a3 u Leu 115 a3 y Lys Thr 130 Phe Al a I I e a3 u As p Val As p Val a31u 1 80 Lys I I e Ser 1 95 a31ya(1ua(1y 210 Al a Al a I I e I I e a3 n Leu Ser a3 y Leu 260 a3 y Phe a3 y 275 Ly s a31u Al a I Ie Ar g Ser ValI Ph e Al a a31u 1 65 Ar g Ar g ValI Pr o As p 245 a31u ValI a3 n ValI Cy s II e a3 n His 55 Ser Al a 70 1 1 e Leu a3 u Thr Lys Ser Leu Al a 135 As p Al a 150 As n II e Al a a3 n Lys Ser a31na(1n 215 Pro a31n 230 Thr Thr Lys Val a3 u Val Al a Asp Leu 10 Lys Lys Leu 25 a3 u Leu Cys 40 Aspa1ua(1n Phe Leu Asp Ser Val Al a 90 Ilie a31y Ar g 1 05 Asn Il e Leu 120 a3 n Thr Leu Thr Ser Leu Leu Leu Lys 1 70 Ar g a31y Ilie 1 85 a31u Asn Pr o 200 Al a Leu Leu a31y a31y Ar g Asn Il e Leu 250 Il e Al a Asp 265 Ser Ser Lys 280 Le u I Ie As n a31u Thr 75 ValI Ar g vb t Al a Thr 1 55 Le u I Ie Ser Ly s Ly s 235 Ph e Ar g Ly s Ly s Al a a31u Ar g Tyr Tyr Ar g Le u Ly s 140 a31u Le u Tyr I Ie I Ie 220 Hi s I Ie As n a31u Cys a31y lIe As n ValI As n As n a31y 125 Le u Al a a31n I Ie Th r 205 Le u Pr o ValI a31y a31u 285 Ser a31y li1e a31u I Ie Hi s As p 110 Pr a Le u a31y Al a As p 1 Ar g a31u As n Al a Ly s 270 Al a Ph e Al a a31u Le u G y Tyr a31u Thr As p Tyr Al a 1 a31u As p a31y a31n a31y 255 Ly s As n Cys ValI a31u Pr a G n Ly s Pr a a31y ValI ValI 1 As p ValI ValI Thr As p 240 Al a ay le Val Asp I Ie Phe Lys Asp Val Leu Pro a31u Asp Leu Val Lys Phe a31y Page 97 Leu 305 Asn Ser Leu G u Leu 385 Val Leu Pr o Asp Val Phe 355 Lys Pr o SIle Al a Phe Lys 325 G n Asp Gy Met Gy 405 Al a SIle 310 Ser Tyr Asp Al a Tyr 390 Al a G u 295 Gy Leu Ar g Al a Ar g 375 Asp Val G u seql i st cor r ect ed. t xt 300 Arg Leu Pro Val Val Ala 315 Val Lys Val Leu Thr G u 330 Arg Leu Phe G u Iet Asp 345 Leu u u lie Ala Asn 360 365 Sy Leu Arg Ala lie IVet 380 Leu Pro Asp Arg Lys Asp 395 Ala Arg Gy u Ala u 410 Lys Thr Al a 425 Val Ar g 335 Al a Al a G u Gy G u 415 <210> 73 <211> 1065 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1042) <223> RXA00744 <400> 73 tctgagtcgg tagaagtatt acccagtgac ttaagtttct cgaccagcca cgt t agtgtg gtcgagtaga ggatagctac gag G u at t l Ie gcg Al a t at Tyr gat Asp cat Hi s act Thr caa G n caa G n gcg Al a tcg Ser ttg Leu gaa G u aat Asn ct g Leu tta Leu aat Asn cag Sn tct Ser gtt Val gaa G u aat Asn act Thr aaa Lys at t SIle gca Al a act Thr cga Ar g aaa Lys ct c Leu tac caa Tyr G n 15 gta agc Val Ser ggt cga G y Arg cga ttt Arg Phe aac agt Asn Ser 80 gat ctt Asp Leu 95 Page 98 tagatttttt tgagcaacag atg ggg aac tgg gca IVet y Asn Trp Ala 1 gat aat cag tac aag Asp Asn G n Tyr Lys gat aaa cgt aga gaa Asp Lys Arg Arg G u aat gta atc gtc tat Asn Val lie Val Tyr t cc ggg caa tct acg Ser G y G n Ser Thr ttt atg act gcg ctc Phe IVet Thr Ala Leu at c ctc cac act ccg Ile Leu His Thr Pro 100 seql i st cor r ect ed. t xt ggt gga gat gtt gct gcg aca gag tcg tta gta gat Gy y Asp Val Ala Al a Thr u Ser Leu Val Asp tac att cac gca Tyr Ile His Ala 105 ct c Leu gca Al a cat Hi s 150 cac Hi s aat Asn ccc Pr o at g Met aaa Lys 230 gtt Val ct g Leu tta Leu cca Pr o cag G n 310 ggt caa gat Gy n Asp 120 aca at g at c Thr Met Ile agt ctt ggc Ser Leu G y tta ttg gaa Leu Leu G u 170 cag act gct G n Thr Al a 185 atg ttg ggt IVet Leu G y 200 aag cag tgg Lys G n Trp gaa aaa gcc G u Lys Al a ctt gcg cat Leu Al a Hi s 250 ttg aat atc Leu Asn Ile 265 ctt act ct t Leu Thr Leu 280 att aag ttt Ile Lys Phe cat gaa aac His G u Asn tt c Phe gca Al a ccc Pr o 155 gaa G u cat Hi s gaa Gu ctt Leu act Thr 235 aat Asn aaa Lys cac Hi s gt c Val aga Ar g ctt Leu 140 at t SIle ttt Phe at a SIle gct Al a gaa G u 220 cgc Ar g cga Ar g gaa G u cac Hi s gt c Val gt c Val 125 tcg Ser gat Asp gag G u tgg Tr p aaa Lys 205 aag Lys gct Al a cac Hi s ctt Leu ct g Leu 285 aat Asn 110 at t SIle t cc Ser cct Pr o caa G n cag G n 190 aaa Lys ggt Sy at c l Ie at t SIle gag G u 270 t cc Ser cat Hi s gt c Val aaa Lys cag G n gcg Al a 175 gt g Val gct Al a at g Met aaa Lys tcg Ser 255 agc Ser gtt Val gac Asp ccc Pr o gag G u ttt Phe 160 aag Lys at c SIle at t l Ie ttt Phe gag G u 240 gt c Val gat Asp at t SIle aac Asn caa G n at t SIle 145 aac Asn aaa Lys ttg Leu cag G n tta Leu 225 ct c Leu agt Ser cca Pr o gct Al a cgt Ar g 305 ct c Leu 130 gtt Val ggc G y gag G u aat Asn tgg Tr p 210 gac Asp gct Al a aaa Lys aag Lys gcg Al a 290 ggc G y 115 gca Al a at g Met ct a Leu gt c Val aaa Lys 195 t cc Ser gag G u gat Asp gca Al a ctt Leu 275 caa G n act Thr at g Mbt ggg G y ccg Pr o tct Ser 180 t ac Tyr aac Asn cct Pr o cat Hi s ctt Leu 260 caa G n cga Ar g ttt Phe t ca Ser aag Lys gca Al a 165 gag G u aac Asn tcg Ser gac Asp t cc Ser 245 gag G u gat Asp gga Sy ct g Leu 451 499 547 595 643 691 739 787 835 883 931 979 1027 1065 300 taattaagtg atgcaatagt ct a <210> 74 <211> 314 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 74 IVet y Asn Trp Ala Gu lie Thr Asp Ou Ile Ser Lys Ile Tyr n 1 5 10 Asp Asn G n Tyr Lys Ile Arg n lie Asn Asp Val Asp Ala Val Ser 25 Page 99 seqi i st car r ect ed. t xt Asp Lys Arg Arg Su Al a Leu a nAl a Leu Phe G u Hi s Thr G y 40 As n Ser Ph e I Ie As p a31n I Ie 145 As n Ly s Le u a31n Le u 225 Le u Ser Pr o A a Ar g 305 I Ie a31n Thr Hi s I Ie 115 A a vb t Le u ValI Ly s 1 95 Ser a31u As p A a Le u 275 a31n Thr Tyr Thr Le u Pr o A a Ser Ly s A a 1 65 a31u As n Ser As p Ser 245 a31u As p a31y Le u Tyr As p 70 Hi s a31y Le u A a Hi s 150 Hi s As n Pr o vb t Ly s 230 ValI Le u Le u Pr o a31n 310 Ser Ph e Ly s a31y Ph e a31y 135 Ser (31y Pr a Thr ValI 215 a31u Thr a31y ValI Le u 295 a31y A a Ser Le u As p a31y 120 Thr Ser Le u a31n vb t 200 Ly s a31u Le u Le u Le u 280 li1e Hi s Tr p ValI As p ValI 1 a31n vb t Le u Le u Thr 1 Le u a31n Ly s A a As n 265 Thr Ly s a31u Le u As n a31n 90 A a As p I Ie a31y (31u 1 70 A a a31y Tr p A a Hi s 250 li1e Le u Ph e As n a31u As p Ser A a Ph e A a Pr a 1 (31u Hi s a31u Le u Thr 235 As n Ly s Hi s ValI a31y As p a31y a31u ValI 125 Ser As p (31u Tr p Ly s 205 Ly s A a Hi s Le u Le u 285 As n Ar g As n As p Le u ValI Ly s a31n A a 1 ValI A a vb t Ly s Ser 255 Ser ValI As p <210O> <21 1> 957 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (934) <223> RXA00844 <400> ggtgacggcc aaaggcgcgc gcattgcgcg ccccaaaatc gacgtcattg acagcatctt Page 100 seql i st cor r ect ed. t xt gtgacaacat tttgtagagc aaccatctag actgttcttt ttt Phe gcc Al a aac Asn at c SIle at c SIle at t SIle ttg Leu agt Ser aat Asn gtt Val 150 cca Pr o ggt Sy caa G n ct a Leu tgg Tr p 230 ccc Pr o acc Thr tta Leu acg Thr aac Asn gcc Al a t ca Ser cct Pr o aac Asn 120 caa G n cag G n ccc Pr o ct c Leu gct Al a 200 ggc G y gcc Al a gaa Gu gca Al a gcg Al a t ca Ser gca Al a cac Hi s act Thr gcg Al a gtt Val gcc Al a gcc Al a cgc Ar g 170 cca Pr o gt g Val gt c Val t ac Tyr gtt Val 250 ct g Leu gcg Al a agc Ser tgt Cys tgt Cys 75 tt g Leu gac Asp gat Asp ggc Gy gat Asp 155 acc Thr ggc G y ct c Leu ggc G y acc Thr 235 gac Asp t cc Ser t ca Ser acc Thr aca Thr at c SIle agc Ser tta Leu ct c Leu tgg Tr p 140 gt g Val gct Al a gat Asp agc Ser tgg Tr p 220 ggc G y gcc Al a gt a Val cct Pr o act Thr 45 gga G y cct Pr o caa G n gct Al a t ac Tyr 125 ggt G y caa G n gt g Val t cc Ser at g Met 205 t ac Tyr aag Lys acc Thr ct c Leu gca Al a t ca Ser acc Thr gag G u ttt Phe ct c Leu 110 ggt G y ggg G y at t SIe ct g Leu ggc G y 190 t cc Ser at c SIle cac Hi s gcc Al a gca Al a 15 cat Hi s gat Asp at g Met ggc G y cag G n 95 gtt Val gca Al a t ac Tyr caa G n ctt Leu 175 gga G y act Thr ccc Pr o at t SIle aac Asn 255 gct Al a gct Al a t ca Ser at c SIle ggt G y 80 cag G n gag G u cac Hi s tct Ser cgc Ar g 160 gaa G u cct Pr o gac Asp gtt Val gcc Al a 240 ccc Pr o at g Met 1 tta Leu ttg Leu at t SIle acc Thr at t SIle gt g Val ctt Leu gt g Val gcc Al a 145 agg Ar g ggc G y ttg Leu gt a Val gct Al a 225 ccc Pr o acc Thr tct tct gcg Ser Ser Al a acg gct gcg Thr Al a Al a gca aat gct Al a Asn Al a gtt cgt ctg Val Arg Leu cca acg tgg Pro Thr Tr p gcc ggt gca Al a G y Al a t cc caa gcg Ser G n Al a 100 ccc aat cag Pro Asn n 115 cag cct ggt G n Pro G y 130 ttt ggc caa Phe G y G n gt a gt c aat Val Val Asn act gtt tct Thr Val Ser 180 t ac at c aat Tyr Ile Asn 195 gaa aac gat G u Asn Asp 210 gaa cac gcc G u Hi s Al a att gct ggt i e Al a G y ttc atc cct Phe IIe Pro 260 t ca Ser t ct Ser cgc Ar g cac Hi s gcg Al a gct Al a at c SIe gca Al a gaa G u aat Asn gtg Val 165 aac Asn ggt G y gca Al a gag G u gcg Al a 245 gct Al a Page 101 seqi i st car r ect ed. t xt cca cag cct t tc acc ggt t ca t cc at c ggt ggt t gg gcg ct g ggc agc Pro O1n Pro Phe Thr Oy Ser Ser lie Oly O3yTrp Ala Leu Oy Ser 265 270 275 t cc t agaat at gc t gat ct ccct gct Ser <210> 76 <21 1> 278 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn <400> 76 l'bt Ser Ser Al a Ser Phe Thr Thr Le1 Le u le Thr li1e ValI Le u ValI Al a 145 Ar g O31y Le u ValI Al a 225 Pr a Thr Al a As n 35 Ar g Thr O31y O31n As n 115 Pr a O31y ValI ValI I Ie 1 95 As n Hi s Al a I Ie Ser Ar g Hi s Al a Al a li1e Al a O31u As n ValI 1 65 As n O31y Al a O31u Al a 245 Al a Al a As n lIe lIe 70 lie Le u Ser As n ValI 150 Pr a O31y O31n Le u Tr p 230 Pr a Pr a Pr a ValI G y Thr G3y Hi s Ser G3y 135 Al a Ser Ar g Le u As p 215 li1e Al a O3n Le u Thr 40 As n Al a Ser Pr a As n 120 O31n O31n Pr a Le u Al a 200 O31y Al a O31u Pr a Lys Al a 10 Val Ala 25 O3 y Ser Thr Al a Ar g Hi s Ser Thr 90 Thr Al a 1 05 Thr Val Al a Al a O3 n Al a Asp Arg 1 70 Val Pr o 1 85 O3 y Val Thr Val Ty r Ty r Leu Val 250 Phe Thr 265 Le u Al a Ser cys cys 75 Le u As p As p O31y As p 1 55 Thr O31y Le u O31y Thr 235 As p O31y Ser Ser Thr Thr li1e Ser Le u Le u Tr p 140 ValI Al a As p Ser Tr p 220 O31y Al a Ser ValI Pr a Thr G y Pr a O3n Al a Tyr 125 Oy On ValI Ser vb t 205 Tyr Lys5 Thr Ser Le u Al a Ser Thr O31u Ph e Le u 110 O31y O31y I Ie Le u O31y 1 Ser I Ie Hi s Al a I Ie 270 Al a Hi s As p vb t O31y O31n ValI Al a Tyr O31n Le u 1 O31y Thr Pr a I Ie As n 255 O31y Al a Al a Ser I Ie O31y O31n O31u Hi s Ser Ar g 1 O31u Pr a As p ValI Al a 240 Pr a O31y Tr p Al a Leu O31y Ser Ser Page 102 seql i st cor r ect ed. t xt 275 <210> 77 <211> 958 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(958) <223> RXA01151 <400> 77 ccaggcgttt ggctgatggc gtggagcctc ctcgtccgca aagacctagt tggagggcgt aaagggttag agtggtgacc gcgtaaggca cgtcgataaa atg agt t IVet Ser S ca cca act ;er Pro Thr gat Asp t cc Ser ctt Leu gat Asp cca Pr o gcg Al a at g Met gaa G u tta Leu gat Asp 150 gat Asp tcg Ser tta Leu gca Al a ttg Leu gt g Val at c l Ie tgt Cys aat Asn 120 acg Thr tcg Ser acc Thr tct Ser gat Asp aat Asn gt g Val ggc Gy gct Al a gcg Al a gtt Val at g Met gag G u aag Lys 170 aat Asn gag Gu gcc Al a ct g Leu act Thr 75 ggt Gy gat Asp ctt Leu gaa G u gag G u 155 ct g Leu tct Ser gt g Val aag Lys ttt Phe ttc Phe gga Gy ttg Leu gag G u at g Met 140 cgc Ar g ccg Pr o agc Ser cag G n gcg Al a cag G n gaa G u cgc Ar g ccg Pr o 110 tgt Cys ggc Gy gt c Val ccc Pr o ttc Phe aag Lys at g Met gag G u gcg Al a 80 cgt Ar g gt g Val ttt Phe gat Asp ctt Leu 160 gac Asp gcc Al a 1 aac Asn cgc Ar g ct g Leu gat Asp aaa Lys gt g Val cag G n gaa G u cgt Ar g 145 gct Al a acc Thr cgg Ar g acc Thr aag Lys at g Met gac Asp gct Al a gtt Val caa G n 130 gct Al a ggt Gy ct g Leu gaa G u tct Ser tct Ser cag G n tct Ser tct Ser 100 ttg Leu ggt Gy gtt Val ttg Leu gtt Val 180 cag G n gat Asp cgg Ar g ccg Pr o aat Asn cct Pr o ggt Sy gaa G u t ca Ser at g Met 165 gac Asp cgc aaa gcg Arg Lys Ala ggc tac gct ttt gag gcg Sy Tyr Al a Phe G u Al a 185 190 aag acg gaa att tcg Lys Thr G u Ile Ser 195 Page 103 IND seqI i st car r ect ed. t xt agg ct t gcg ctg gaa gag gcg cca gat gt g t ct t at cag gat at t ggt 739 SAr g Leu Al a Leu O31u O31u Al a Pr o Asp Val Ser Tyr O31n Asp Ilie G31y 200 205 210 fl ggc t tg gat gat cag at t gaa t tg at t caa gat gcc gt t gag ct g cca 787 31 Gy Leu Asp Asp (1n Ile (1u Leu Ile (1n Asp Ala Val G31u Leu Pr o 215 220 225 t tt t tg cac ccg gag at g t ac cgc gcc t ac aac ct g cat cca cca aag 835 Phe Leu Hi s Pr o O31u I'bt Tyr Ar g Al a Tyr Asn Leu Hi s Pr o Pr o Lys 230 235 240 245 C1 ggc gt g ct g ct g t ac ggc cct ccc ggc t gt gga aag acg ct g at t gct 883 (3 OyVal Leu Leu Tyr (1y Pr oPr o 1y Cys (1y Lys Thr LeulIleAl a 00 250 255 260 Saag gct gt g gct aat t ct t tg gcc aac cgc at c ggt gag act ggc acc 931 C1 Lys Al a Val Al a Asn Ser Leu Al a Asn Ar g Ilie 31y 31u Thr G31y Thr ID265 270 275 t tcg t ac t tc at c aac gt c aag ggg cca 958 K1 Ser Tyr Phe Ilie Asn Val Lys G31y Pr o 280 285 <210> 78 <211> 286 <21 2> PRT <21 3> Cor ynebact er i urn gI ut anri cumn <400> 78 I'bt Ser Ser Pro Thr Asp Ser Ser Pro Ser Asn Ser Phe Ser Asp Phe 1 5 10 Asn Ar g (31u 31u O31n Ser Arg Leu Ser Asp (31u ValI Ar g O31n Leu Lys 25 Ar g Thr Asn Ser Asp Leu G31y Al a Ar g Asn Ala Lys L eu Al a O31u Ib t 40 Leu Lys Ser Ser Arg Asp Lys Leu Ser Val Leu Phe Ser 03 n Leu 03 u 55 Asp I'b t Al a O31n Pr o Pr o Ser Val Tyr G31y Thr Phe Leu O31u Thr Al a 70 75 Lys Asp O31y Ser Asn Al a O31u Ilie Phe Al a 31y O31y Ar g Ar g I'b t Ar g 90 Val Al aVal Ser Pr o I'b t Leu Cys Al aAl aAsp Leu I'b t Pro O31y Val 100 105 110 31n Val Ar g Leu (1y (1u (1y Asn (1n Val Leu (1u Al aCys Asp Phe 115 120 125 (31u 1n Thr (31y (1u Leu Al aThr Leu 'b t O31u 'b t Ile (1y Ar gAsp 130 135 140 Ar g Al a Leu Val Ser Asp Ar g Ser 31y O31u O31u Ar g Val Val Lys Leu 145 150 155 160 Ala O31y Pr o Leu I'b t Asp Ar g Thr Al a Lys Leu Pr o Ar g Pr o O31y Asp 165 170 175 Thr Leu Leu Val Asp Ar g Lys Al a O31y Tyr Al a Phe O31u Al a Ilie Al a 180 185 190 Lys Thr O31u Ilie Ser Ar g Leu Al a Leu O31u O31u Al a Pr o Asp Val Ser Page 104 Ty r G3 n 210 A a Val 225 Leu Hi s Lys Thr (31y (1u 1 95 Asp II e Gy G y Leu 215 G31u Leu Pro Phe Leu 230 Pr o Pr o Lys G31y Val 245 Leu Il e A a Lys A a 260 Thr G3 y Thr Ser Tyr 275 seqI i st car r ect ed. t xt 200 205 Asp Asp aOn Il e a u Leu 220 Hi s Pr o G31u Nt Tyr Ar g 235 Leu Leu Tyr a31y Pr o Pr o 250 Val Ala As n Ser Leu Ala 265 Phe I Ie As n Val Lys a31y 280 285 I I e a3 n As p A a Ty r As n 240 31y cysa(1y 255 As n Ar g I Ie 270 Pr o <210O> 79 <21 1> 735 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 OIDS <222> (712) <223> RXA02317 <400> 79 gaattt tccg gt gt taaaat gt gcggt gag ct tggcgtt g gacgt cgaaa agcgaat tca t ggccccat c t tgcct taaa gcggaggacc agtgtgggga at g gcg cac at g I'bt A a Hi s I'bt tt a Le u ggt a31y ccc Pr a c ac Hi s g aa a31u ggt a31y gag a31u ag c Ser ct g Le u gt g ValI gct A a tt g Le u g at As p act Thr tt g Leu ggc a31y 120 t cc Ser t at Tyr cct Pr a at t I Ie gt g ValI g at As p aag Ly s gt a ValI ggc a31y cct Pr a at g vb t gag a31u ggt a31y at g vb t gt t ValI gca A a g at As p gcg A a ac g Thr ccg Pr a gt t ValI ct g Le u gct A a 110 cct Pr a at t I Ie ag g Ar g cga Ar g t cc Ser ggg a31y cgt Ar g ct a Le u at t I Ie 1 cgg Ar g ct t Le u aat As n ac g Thr gt g ValI t cc Ser ccg Pr a g aa a31u g at As p tt t Ph e at g vb t t cg Ser t at Tyr ac a Thr t at Tyr ccg Pr a 130 gt a ValI g at As p cgc Ar g ccg Pr a gcg A a aat As n 1 00 gga a31y ag c Ser t tg gat agc ccc gat gt c gcg ccg aat t ta agc gac t at t ca ggt ct a Page 105 Le u ggc G31y 1 50 c aa (31n g aa G31u g ac As p Ser t gc cys ccc Pr o ccg Pr o G31y 200 As p cat Hi s g at As p 1 70 gt c ValI c ac Hi s ValI gt c ValI 155 gt g ValI ct g Le u ct a Le u A a 140 gt g ValI tt t Ph e ct t Le u at t I Ie seqI i st car r ect ed. t xt Pro Asn Leu Ser Asp Tyr Ser G31y Leu 145 gt g ccc cat gct ggt ggc acg at c ccg Val Pr o Hi s Ala 31y G31y Th r Ilie Pr o 160 165 gcg gaa acc gt g cgc acc t ac ggc gcc Ala G31u Thr Val Ar g Thr Tyr G31y Ala 175 180 aaa gat gga cag gca ct g ct t at c gac Lys Asp 03 y 03 n A a Leu Leu Il e Asp 190 195 t aggat ggt t ccccat gagc acc <210O> <21 1> 204 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> [vb t A a Ar g As p Leu Phe As n I'b t Thr Ser Val Ty r Ser Thr Pr o Ty r G31u Pr o 130 Asp Tyr 145 G31YG1Y. Ar g Thr A a Leu Hi s I'bt Phe Val A a As p Leu Arg Thr Pr o Val Ala (31y As n 1 00 I Ie G31y 115 1 1 e Ser Ser 03 y Thr I Ie Ty r 03 y 1 80 Leu I I e 1 95 Ar g Ar g Ser G31u G31u G31y As p Thr Ph e Leu Pr o 1 65 A a As p Leu Leu Leu Thr Thr G31u G31y 55 ValI G31u ValI A a As p 135 Le u Ph e Ph e Hi s ValI A a 40 Le u As p Thr Le u G31y 120 Ser cys Pr a Pr a G31y 200 A a 25 A a Ser Ar g Ph e I Ie 1 05 A a Pr a Gu le Le u 1 85 ValI Ph e I Ie Ph e Ar g Le u Le u Hi s I Ie ValI ValI 1 ValI Le u Le u G31y Pr a vb t G31u G31y vb t ValI A a A a 140 ValI Ph e Le u I Ie Hi s As p G31u Le u G31u Tr p Ar g G31y 125 Pr a ValI A a Ly s As p A a Thr Pr a ValI Le u A a 110 Pr a As n Pr a G31u As p 1 Hi s Thr Gu le As p Le u G31y Ser Le u Hi s Th r 1 G31y <210O> 81 <21 1> 774 Page 106 seal i st cor r ect ed. t xt <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(751) <223> RXA02644 <400> 81 cgtggggaag tggagcccac actgaaaagc tttgcgaggt tgaaaatgct gccggttggc gagctaagat gtgagatgct caacatggcc accaggctag atg gca cat ctc acg 11! Met Ala His Leu Thr caa tac caa G n Tyr n atc aag cgc Ile Lys Ar g gag cag gct G u G n Al a gcg cgt cat Al a Ar g Hi s gat gt g gag Asp Val u aaa ccc at g Lys Pro IVet gct gtg gtg Al a Val Val tta gtt aat Leu Val Asn 120 ttg caa gt c Leu G n Val 135 cat t ct gat Hi s Ser Asp 150 atc att act Ile Ile Thr tta ttg cct Leu Leu Pro atg ggt gca Vet y Al a 200 cct Pr o cga Ar g gaa G u tgc Cys t cc Ser gag G u cgt Ar g t ac Tyr cgc Ar g ggg G y act Thr 170 gaa G u at t l Ie cag G n ttt Phe ttt Phe agt Ser tct Ser 75 gcg Al a t at Tyr acc Thr tct Ser cgc Ar g 155 gaa G u cag G n gaa G u gcc Al a ct g Leu at t SIle gcc Al a 60 gat Asp ttg Leu ttc Phe aac Asn gtt Val 140 at t SIle t at Tyr gct Al a ttg Leu ggt G y acc Thr cac Hi s 45 ttt Phe gat Asp cgt Ar g ggt G y gcg Al a 125 cgg Ar g gaa G u ggt G y gcg Al a aaa Lys 205 caa G n t at Tyr t cc Ser at t SIle ggc G y ggc G y ggc G y 110 gt g Val gag G u gcg Al a gca Al a gt g Val 190 gaa G u gt c Val 15 at c SIle at c SIle ttc Phe gaa G u tcg Ser 95 gt a Val acg Thr at t SIle aat Asn gaa G u 175 gaa G u t cc Ser ttt Phe acg Thr aag Lys cat Hi s cct Pr o 80 gga G y aaa Lys gag G u ttc Phe ct g Leu 160 gt c Val tct Ser ggg G y 1 gag G u cgt Ar g gag G u gt g Val t cc Ser at g Met ct g Leu ctt Leu aag Lys 145 cgc Ar g aca Thr caa G n cac Hi s cat Hi s gt g Val ct g Leu gat Asp ggt G y aaa Lys ggc G y ct a Leu 130 at t SIle ggc G y t ac Tyr ttg Leu at g Met 210 gaa G u caa G n t at Tyr ggc G y acc Thr gat Asp act Thr 115 cct Pr o gac Asp at g Met acc Thr t ca Ser 195 tgg Tr p ct a Leu gat Asp ccg Pr o t ca Ser gcg Al a at t SIle 100 ggc G y gag G u ctt Leu ggc G y ttg Leu 180 t cc Ser gt g Val gag G u cag G n gat Asp aat Asn gga G y gcc Al a gga G y gtt Val ccg Pr o at c SIe 165 gct Al a at g Met gaa G u tcc ccg agt gac tagtgcggtg taagagcact aga Page 107 seql i st cor r ect ed. t xt Ser Pro Ser Asp 215 <210> 82 <211> 217 <212> PRT <213> Corynebacteri um <400> 82 let Ala G gl ut ai cum n Tyr G n Leu Pro G n Ala y G n Val Phe Hi s Leu Thr 1 G u Ar g G u Val Ser let Leu Leu Lys 145 Ar g Thr G n Hi s G u G n Tyr Gy Thr Asp Thr 115 Pr o Asp let Thr Ser 195 Tr p 5 G u G n Asp Asn Gy Al a Gy Val Pr o SI e 165 Al a let G u SIle G u Al a Asp 70 Lys Al a Leu Leu Hi s 150 I le Leu bet Ser Lys G n Ar g Val Pr o Val Val G n 135 Ser SIle Leu Sy Pr o 215 Ar g Al a 40 Hi s G u Met Val Asn 120 Val Asp Thr Pr o Al a 200 Ser 10 Ser Arg Arg G u His Cys Arg Ser Leu G u 90 Val Arg 105 Al a Tyr Thr Arg Al a G y Asp Thr 170 G y G u 185 Su lie Asp Phe Phe Ser Ser Al a Tyr Thr Ser Ar g 155 G u G n G u Leu SIle Al a Asp Leu Phe Asn Val 140 I le Tyr Al a Leu Thr Hi s Phe Asp Ar g Sy Al a 125 Ar g G u Sy Al a Lys 205 Tyr Ser SIle Sy Gy Sy 110 Val G u Al a Al a Val 190 G u I e I e Phe G u Ser Val Thr SIe Asn G u 175 G u Ser <210> 83 <211> 1411 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1411) <223> RXN02820 <400> 83 caacgaaaca aatgcaagcc cccaatcatg ggtttctacc aattaaattt tctttcagaa aatatctccc cacataaaag ttccttgata ggctcgagag atg aaa gtg acc caa 115 lMt Lys Val Thr G n Page 108 NO seql i st cor r ect ed. t xt 1 Sagc aca ttc ctt aaa tcg gta gct gcg ttc act gtc gca gcc tta acc 163 Ser Thr Phe Leu Lys Ser Val Ala Ala Phe Thr Val Ala Ala Leu Thr ,C 10 15 4 ctg acc atc tct tcg tgt tcc agc ggt gaa gac acc tcc gca agc tcc 211 Leu Thr Ile Ser Ser Cys Ser Ser G y u Asp Thr Ser Ala Ser Ser 30 acg gat act gaa aac tcc tca acc caa gca gca gcg tct ccc cca ctt 259 Thr Asp Thr u Asn Ser Ser Thr n Ala Ala Ala Ser Pro Pro Leu ri 40 45 00 gcg cct tgt gaa ctt ccc gcc gac gct tct gct gaa gag gaa gta gaa 307 SAla Pro Cys u Leu Pro Ala Asp Ala Ser Ala u u u Val u 60 .C ggc act cac aca ggt gaa gat att tct gtt gcc ccg gaa at c ggt acc 355 (3 y Thr His Thr 3 y 3 u Asp Ile Ser Val Ala Pro 3 u Ile 3 y Thr S 70 75 80 ggc tac cgc gag ggc atg acc cct gtt caa acc caa ggt tat gcg gtg 403 G y Tyr Arg u G y Ibt Thr Pro Val n Thr n G y Tyr Ala Val 95 100 gca act gca aac ccc atc gct tct gaa gca gcc tgc gcg gtg tta aga 451 Ala Thr Ala Asn Pro Ile Ala Ser u Ala Ala Cys Ala Val Leu Arg 105 110 115 gaa ggc ggc act gca gct gat gct ctt gtc acc gcg cag ttt gtt ttg 499 Su y y Thr Ala Ala Asp Ala Leu Val Thr Ala n Phe Val Leu 120 125 130 gga ctg acg gaa ccg cag tcg tct ggc ctt ggt ggt ggc gga tac att 547 Sy Leu Thr u Pro n Ser Ser y Leu y y y y Tyr Ile 135 140 145 ctg tac tac gac gcc gaa gcc aat gcg gtg aca gcc att gat ggc cgt 595 Leu Tyr Tyr Asp Ala u Ala Asn Ala Val Thr Ala le Asp y Arg 150 155 160 165 gaa aca gcg cca gtt gct gct gat gaa aac tat ctc att cat gtt tct 643 Su Thr Ala Pro Val Ala Ala Asp u Asn Tyr Leu Ile His Val Ser 170 175 180 gca gag gat caa acg gca cct gtt cct gat gcc cga cgt tcc ggc agg 691 Ala u Asp n Thr Ala Pro Val Pro Asp Ala Arg Arg Ser y Arg 185 190 195 tca att ggt gtg cca gga atc gtg gca gcc ctt gga cag ctg cat gat 739 Ser Ile G y Val Pro 3 y Ile Val Ala Ala Leu 3 y 3 n Leu His Asp 200 205 210 tca ttc gga aag acc tcc tgg cag gac gtg ctg aca act ccg cag cag 787 Ser Phe G y Lys Thr Ser Trp n Asp Val Leu Thr Thr Pro n O n 215 220 225 ctc gca act gat ggt ttt tcc atc agc cct cgc atg tca gca tca att 835 Leu Ala Thr Asp y Phe Ser Ile Ser Pro Arg 'b t Ser Ala Ser Ile 230 235 240 245 gct aac t cc gct gag gat ctc t cc cac gat ccg gaa gct gcc gca tat 883 Ala Asn Ser Ala u Asp Leu Ser His Asp Pro u Ala Ala Ala Tyr 250 255 260 ttc ctt gat gaa aac ggt gat gcg aag gca ccc ggc aca ctt tta caa 931 Phe Leu Asp u Asn y Asp Ala Lys Ala Pro y Thr Leu Leu n Page 109 seql i st cor r ect ed. t xt 270 aac Asn gat Asp acc Thr 310 ttg Leu cgc Ar g aca Thr t ac Tyr gct Al a 390 gat Asp gt c Val gac Asp 280 ttc Phe gag G u gcc Al a aag Lys at g Met 360 ccc Pr o cac Hi s t ac Tyr cag G n gca Al a acg Thr gac Asp act Thr 330 gtt Val acc Thr gag Gu at t SIle ggt Gy 410 at c SIle gaa Gu ggt Gy ggt Gy 315 ccg Pr o tgt Cys ct g Leu gtt Val t ca Ser 395 gat Asp aac Asn acg Thr gag G u 300 ttc Phe gaa G u ggc Gy ggt Gy ggt Gy 380 gag Gu cct Pr o cat Hi s at t SIle 285 at t SIle aca Thr act Thr at g Met at c SIle 365 ttg Leu gct Al a gct Al a gt c Val cgt Ar g gca Al a cca Pr o cgt Ar g cca Pr o 350 ttg Leu gat Asp gag Gu ttc Phe cac Hi s 430 ct c at c t ct Leu II e Ser gca gac at c Al a Asp I e 305 tca ct g at g Ser Leu IVet 320 gaa gct ttg G u Al a Leu 335 ccg t ca t ca Pro Ser Ser aac aac ttt Asn Asn Phe ggc gga ttg G y G y Leu 385 cgc ctg gct Arg Leu Ala 400 gtg gaa gt t Val G u Val 415 acg ggc gaa Thr G y G u Lys Ser Val 10 Ser Cys Ser Asn Ser Ser Leu Pro Al a G y Gu Asp 75 G y Met Thr 90 Pro lie Ala Page 110 gaa G u 290 gt g Val agc Ser tgt Cys tcg Ser gat Asp 370 cca Pr o t at Tyr cca Pr o cac Hi s Al a Ser Thr Asp SIle Pr o Ser ggt Gy gaa G u acg Thr gct Al a ggt Gy 355 ct c Leu aat Asn gct Al a gca Al a t cc Ser 435 Al a Gy G n Al a Ser Val G u ggc Gy cgc Ar g gca Al a ccc Pr o 340 ggc Gy gcc Al a gcg Al a gat Asp ggt Gy 420 aaa Lys Phe G u Al a Ser Val G n Al a 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 <210> 84 <211> 437 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 84 IVet Lys Val Thr G n Ser Thr Phe Leu 1 5 Val Ala Ala Leu Thr Leu Thr Ile Ser 25 Thr Ser Ala Ser Ser Thr Asp Thr G u 40 Ala Ser Pro Pro Leu Al a Pro Cys G u 55 Su u u Val u y Thr His Thr 70 Pro u lie y Thr y Tyr Arg u G n y Tyr Ala Val Al a Thr Ala Asn Thr Asp Al a Al a Al a Thr Al a seql i st cor r ect ed. t xt 105 Thr Ala Ala Asp Cys Al a Sy 145 Al a Leu Ar g Sy Thr 225 lbt G u Sy Ser SIle 305 l t Leu Ser Phe Leu 385 Al a Val G u Val 115 Phe Gy Asp Hi s Ser 195 Leu Pr o Al a Al a Leu 275 Gy G u Thr Al a Gy 355 Leu Asn Al a Al a Ser 435 Ar g Leu SIle Ar g 165 Ser Ar g Asp G n l Ie 245 Tyr G n Pr o Al a Asp 325 Tyr Val G n G u Ar g 405 Gy Leu Q u Q y G y Leu 135 Leu Tyr 150 G u Thr Al a G u Ser Ile Ser Phe 215 Leu Al a 230 Al a Asn Phe Leu Asn Pro Asp Al a 295 Thr Ar g 310 Leu Al a Arg Asp Thr Val Tyr Pro 375 Al a Val 390 Asp Al a Val G n Gy 120 Thr Tyr Al a Asp Gy 200 Gy Thr Ser Asp Asp 280 Phe G u Al a Lys l t 360 Pr o Hi s Tyr G n Q u Asp Pr o G n 185 Val Lys Asp Al a G u 265 Tyr Tyr Val Tyr SIle 345 G u Thr Leu SIle Tr p 425 Pr o Al a Val 170 Thr Pr o Thr Gy G u 250 Asn Al a Thr Asp Thr 330 Val Thr G u SIle Gy 410 I le n G u 155 Al a Al a Gy Ser Phe 235 Asp Gy G u Gy Gy 315 Pr o Cys Leu Val Ser 395 Asp Asn Ser 140 Al a Al a Pr o SIle Tr p 220 Ser Leu Asp Thr G u 300 Phe G u Gy Gy Gy 380 G u Pr o Hi s Al a 125 Ser Asn Asp Val Val 205 G n SIle Ser Al a SIle 285 I le Thr Thr l t SIle 365 Leu Al a Al a Val Leu Gy Al a G u Pr o 190 Al a Asp Ser Hi s Lys 270 Ar g Al a Pr o Ar g Pr o 350 Leu Asp G u Phe Hi s 430 Val Leu Val Asn 175 Asp Al a Val Pr o Asp 255 Al a Leu Al a Ser G u 335 Pr o Asn Sy Ar g Val 415 Thr Thr Gy Thr 160 Tyr Al a Leu Leu Ar g 240 Pr o Pr o SIe Asp Leu 320 Al a Ser Asn Sy Leu 400 G u Sy <210> <211> 507 Page 111 seql i st cor r ect ed. t xt <212> DNA <213> Corynebact eri um gl ut am cum <220> <221> CDS <222> <223> FRXA02820 <400> gct aac t cc Al a Asn Ser 1 ttc ctt gat Phe Leu Asp aac cct gac Asn Pro Asp gat gcg ttc Asp Al a Phe acc cgt gag Thr Arg G u ttg gct gcc Leu Al a Al a cgc gac aag Arg Asp Lys aca gt g at g Thr Val lMet 115 tac cca ccc Tyr Pro Pro 130 gct gtt cac Al a Val Hi s 145 gat gct t ac Asp Al a Tyr gag Su 5 aac Asn gca Al a acg Thr gac Asp act Thr gtt Val acc Thr gag G u at t SIle ggt Sy 165 gat ctc tcc cac gat ccg gaa gct gcc gca t at Asp Leu Ser His Asp Pro u Ala Ala Ala Tyr 10 ggt Sy gaa G u ggt G y ggt Gy 70 ccg Pr o tgt Cys ct g Leu gtt Val t ca Ser 150 gat Asp gat Asp acg Thr gag G u ttc Phe gaa G u ggc gty ggt Gy ggt Gy 135 gag G u cct Pr o gcg Al a at t SIle 40 at t SIle aca Thr act Thr at g Met at c SIle 120 ttg Leu gct Al a gct Al a aag Lys cgt Ar g gca Al a cca Pr o cgt Ar g cca Pr o 105 ttg Leu gat Asp gag G u ttc Phe gca Al a ct c Leu gca Al a t ca Ser gaa G u 90 ccg Pr o aac Asn ggc Gy ccc Pr o at c SIle gac Asp ct g Leu 75 gct Al a t ca Ser aac Asn gga Gy aca Thr gaa G u gt g Val agc Ser tgt Cys tcg Ser gat Asp 125 cca Pr o ctt Leu ggt G y gaa G u acg Thr gct Al a ggt Gy 110 ct c Leu aat Asn tta Leu ggc G y cgc Ar g gca Al a ccc Pr o ggc Gy gcc Al a gcg Al a caa G n ccc Pr o gcc Al a gat Asp t ac Tyr gtc Val caa G n gaa G u cgc ctg gct Arg Leu Ala tat gct gat cgc Tyr Ala Asp Arg <210> 86 <211> 169 <212> PRT <213> Corynebacter i um gl utam cum <400> 86 Ala Asn Ser Ala u Asp Leu Ser His Asp Pro u Ala Ala Ala Tyr 1 5 10 Phe Leu Asp G u Asn y Asp Ala Lys Ala Pro y Thr Leu Leu G n 25 Asn Pro Asp Tyr Ala G u Thr lie Arg Leu Ile Ser Gu Gy y Pro 40 Page 112 As p Thr Le u Ar g Thr Tyr A a 145 As p Ph e O31u A a Ly s vb t 115 Pr o Hi s Tyr Thr As p Thr ValI Thr (31u I Ie G31y 1 (31u Ph e (31u G31y G31y G31y 135 O31u Pr o seqI i st car r ect ed. t xt Il e Al aAl aAsp Il e Val Thr Pr o Ser Leu I'b t Ser Thr Ar g O31u Ala Leu Cys 90 I'b t Pr o Pr o Ser Ser Ser 1 05 1 1 e Leu As n As n Phe As p 120 125 Leu As p 31y G31y Leu Pr o 140 Ala O31u Ar g Leu Ala Ty r 1 Al a Phe O31u Thr A a G31y 110 Le u As n A a Ar g A a Pr o G31y A a A a As p <210O> 87 <21 1> 604 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (604) <223> FRXA02000 <400> 87 caacgaaaca aatgcaagcc cccaatcatg ggtttctacc aat at ctccc cacat aaaag t tcct tgat a ggct cgagag aat taaat tt t ct t tcagaa atg aaa gtg acc caa 11! I'bt Lys Val Thr 03 n ag c Ser ct g Le u ac g Thr gcg A a ggc G31y ggc G31y tt c Ph e at c I Ie act Thr t gt Cys c ac Hi s cgc Ar g aaa Ly s t cg Ser aac As n ct t Le u ggt G31y G31y gt a ValI t cc Ser t ca Ser gcc A a g at As p ac c Thr gct A a ag c Ser ac c Thr 45 g ac As p at t I Ie cct Pr o gcg A a ggt G31y c aa O31n gct A a t ct Ser gt t ValI tt c Ph e 15 g aa (31u gca A a t ct Ser gt t ValI c aa O31n 95 act Thr g ac As p gca A a gct A a gcc A a ac c Thr 1 gt c ValI ac c Thr gcg A a g aa O31u ccg Pr a c aa O31n gca A a t cc Ser t ct Ser gag (31u g aa (31u ggt G31y gcc A a gca A a ccc Pr a g aa (31u at c I Ie t at Tyr tt a Le u ag c Ser cca Pr a gt a ValI ggt G31y gcg A a 1 00 gca act gca aac ccc at c gct t ct gaa gca gcc t gc gcg gt g t ta aga Page 113 Al a g aa O31u gga G31y ct g Le u 1 50 g aa O31u seqI i st car r ect ed. t xt Al a Asn Pro lieAla Ser Ou Ala Ala Cys Ala Val Leu 105 110 115 ggc act gca gct gat gct ct t gt c acc gcg cag t tt gt t O31y Thr Al a Al a As p Al a Leu Val Thr Al a O31n Phe Val 120 125 130 acg gaa ccg cag t cg t ct ggc ct t ggt ggt ggc gga t ac Thr O31u Pr o O31n Ser Ser O31y Leu O31y O31y O31y O31y Tyr 140 145 tac gac gcc gaa gcc aat gcg gtg aca gcc att gat ggc Tyr Asp Al a O31u Al a Asn Al a Val Thr Al a Ilie Asp O31y 155 160 gcg Al a <210O> 88 <211> 168 <21 2> PRT <21 3> Cor ynebact er i urn gl ut anri curn <400> 88 I'bt Lys Val Val Al a Al a Thr Ser Al a Al a Ser Pr o O31u (1u (1u Pr o O31u IIe O3 n 03 y Ty r Cys Al a Val 115 Al a 03 n Phe 130 G31YG(1YG(1Y 145 Al a I I e As p <210O> 89 <21 1> 824 <212> DNA Th r 03 n Leu Thr Ser Ser Pro Leu Val O31u 03 y Thr Al a Val 1 00 Leu Arg Val Leu Ty r I Ie G31y Ar g 1 Ser Thr Phe Leu Le u Thr Al a G31y 70 G31y Al a O31u G31y Le u 150 O31u Thr As p Pr o 55 Thr Tyr Thr G31y Le u 135 Tyr Thr Ser 25 O31u O31u Thr O31u As n 1 05 Thr O31u As p Ly s 10 Ser As n Le u G31y G31y 90 Pr o Al a Pr o Al a Ser Val Ala Ala Cys Ser Pr a O31u 75 vb t I Ie Al a O31n (31u 1 55 Ser Ser Al a As p Thr Al a As p Ser 140 Al a Ser Thr As p I Ie Pr a Ser Al a 125 Ser As n G31y O31n Al a Ser ValI O31u 110 Le u O31y Al a Ph e O31u Al a Ser ValI O31n Al a ValI Le u ValI Thr As p Al a Al a Al a Thr Al a Thr O31y Thr 1 <213> <220> <221 <222> Cor ynebact er i urn gI ut ani curn OIDS (801) Page 114 seql i st cor r ect ed. t xt <223> RXN03178 <400> 89 ccc Pr o 1 t cc Ser ct g Leu gct Al a ct g Leu ggt G y at t SIle acc Thr gt c Val ggc G y 145 ct c Leu gt c Val t cc Ser agc Ser gac Asp 225 ct c Leu acc Thr gt g Val ggt G y gaa G u aag Lys gat Asp cag G n cac Hi s 115 aac Asn acc Thr cgg Ar g gac Asp ccc Pr o 195 aac Asn ct g Leu gt g Val gt c Val 5 gt c Val ctt Leu ggt G y ttg Leu ccg Pr o gcg Al a gat Asp aac Asn t ac Tyr ggt Sy 165 cgt Ar g acg Thr gt g Val t ca Ser at c SIle 245 acg Thr ggc G y tta Leu ggg G y ggc G y 70 gga G y tgg Tr p tt c Phe gcc Al a acc Thr 150 cgc Ar g ccg Pr o ccg Pr o gca Al a ggt G y 230 gga G y ggc G y gag G u gcc Al a gat Asp act Thr at g let cag G n cct Pr o ttg Leu 135 gac Asp cgc Ar g tgg Tr p ggg G y ccg Pr o 215 gag G u at c SIle acg Thr gcc Al a agc Ser 40 caa G n caa G n tcg Ser aac Asn ggt G y 120 ttc Phe gac Asp ct c Leu gaa G u t cc Ser 200 gcg Al a ggt G y at c SIle at g let ggc G y ggt G y gca Al a gac Asp acc Thr ccg Pr o 105 tgg Tr p at g Met gcg Al a gcc Al a caa G n 185 ggc G y acc Thr ggg G y gt g Val gaa G u 10 cag G n aac Asn acc Thr acc Thr t cc Ser 90 gtt Val ggc G y aac Asn aac Asn gcc Al a 170 gca Al a gt g Val cca Pr o tcg Ser ct g Leu 250 gcc Al a t at Tyr gat Asp ct g Leu ttc Phe 75 gca Al a ttc Phe gac Asp gat Asp cac Hi s 155 gt a Val cga Ar g ggc G y gaa G u cag G n 235 ttg Leu gcc Al a acc Thr gcg Al a gag G u gtt Val t ac Tyr gag G u aat Asn cct Pr o 140 acc Thr ct c Leu ttg Leu cag G n tta Leu 220 aac Asn ct a Leu aac Asn gtt Val gcg Al a aaa Lys gcc Al a gac Asp tcg Ser gag G u 125 gat Asp ttt Phe ttg Leu ctt Leu ct g Leu 205 cca Pr o acg Thr at c SIle gag G u cag G n at g Met aac Asn t at Tyr t ca Ser at c SIle ttc Phe at c SIle ggc G y t cc Ser 175 gac Asp t cc Ser ccc Pr o ctt Leu gca Al a 255 ggc G y ctg Leu ttg Leu gcg Al a t cc Ser ttg Leu t cc Ser caa G n ggc G y ggt G y 160 acc Thr gcc Al a ggc G y acc Thr aag Lys 240 ct a Leu gcg tgg aca ttc aga tct ccc aag aaa aag aac taggtgttct tcttcacgac 821 Page 115 seqi i st car r ect ed. t xt Ala Trp Thr Phe Arg Ser Pro Lys Lys Lys Asn 260 265 ct c <210O> <21 1> 267 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> A1 Pro Thr Thr Val Ser Le u A a Le u Gy le Th r ValI G31y 145 Le u ValI Ser Ser As p 225 Le u A a ValI G31y O31u Ly s As p O31n Hi s 115 As n Thr Ar g As p Pr a 1 95 As n Le u ValI Thr Val Thr G31y Thr l'bt O31u 10 Val 31y O31u Ala 31y O31n Leu Leu A a Ser 03 y As n 40 03 y 03 y As p 03 n A a Th r Leu 03 y Th r 03 n Asp Thr 70 Pr o 31y l'b t Ser Thr Ser 90 Ala Tr p O31n As n Pr o Val 1 05 As p Phe Pro G31y Trp G31y 120 As n A a Leu Phe Nib t As n 135 Ty r Thr As p As p A a As n 150 G31y Ar g Ar g Leu Ala Ala 165 170 Ar g Pr o Tr p O31u O31n Ala 1 85 Thr Pr o O31y Ser O31y Val 200 Val Ala Pr o Ala Thr Pr o 215 Ser 03 y 03 u 03 y 03 y Ser 230 li1e O31y I Ie I Ie Val Leu 245 250 Ar g Ser Pr o Lys Lys Lys 265 A a Tyr As p Le u Ph e A a Ph e As p As p Hi s 1 ValI Ar g O31y O31u O31n 235 Le u As n A a Thr A a O31u ValI Tyr O31u As n Pr o 140 Thr Le u Le u O31n Le u 220 As n Le u As n ValI A a Ly s A a As p Ser O31u 125 As p Ph e Le u Le u Le u 205 Pr o Thr I Ie I Ie As p Tyr ValI Thr Nibt I Ie 110 O31y O31y ValI As p I Ie 1 O31y O31u Le u A a O31u O31n vb t As n Tyr Ser lie Ph e I Ie O31y Ser 1 As p Ser Pr o Le u A a 255 <210O> 91 <21 1> 749 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn Page 116 seqI i st car r ect ed. t xt <220> <221 OIDS <222> (726) <223> FRXA02859 <400> 91 c ag O31n aac As n ac c Thr ac c Thr t cc Ser gt t ValI ggc G31y aac As n aac As n gcc Al a 145 gca Al a gt g ValI cca Pr o t cg Ser ct g Le u 225 ac c Thr gcg Al a gag (31u gt t ValI t ac Tyr gag (31u aat As n cct Pr o 115 ac c Thr ct c Le u tt g Le u c ag O31n t ta Le u 1 95 aac As n ct a Le u gat cag ctg ctg cac ggt Asp 03 n Leu Leu Hi s 03 y 10 t at ct g t tg gct cag gaa Tyr Leu Leu Al a 03 n 03 u gt a aac gcg ct g gcc aag Val Asn Al a Leu Al a Lys 40 act t at t cc ggt t tg gat Thr Tyr Ser 03 y Leu Asp at g t ca t tg at t t at cag I'b t Ser Leu Ilie Tyr O31n 70 at t at c t cc acc gat cac Il e Il e Ser Thr Asp Hi s 90 ggt t tc caa gt c t gg aac G31y Phe O31n Val Tr p Asn 1 ggc at c ggc ggc aag acc O3 y Il e 03 y 03 y Lys Thr 120 gt c ggc ggt ct c gat cgg Val O31y O31y Leu Asp Ar g 135 gat tcc acc gtc agc gac Asp Ser Thr Val Ser Asp 150 at c gac gcc t cc ct c ccc Ilie Asp Al a Ser Leu Pr o 165 170 ggc tcc ggc agc gcg aac O3 y Ser 03 y Ser Al a Asn 1 gaa ccc acc gac aac ctg O31u Pr o Thr Asp Asn Leu 200 ct g ct t aag ct c gt g gt g Leu Leu Lys Leu Val Val 215 gcc gca cta gcg tgg aca Al a Al a Leu Al a Tr p Thr 230 ct t ct t t ta gcc Leu Leu Leu Al a ct t Le u gag O31u gcg Al a cat Hi s 75 at t I Ie g at As p ggc O31y ggt O31y at t I Ie 1 55 at c I Ie g at As p act Thr ccc Pr o tt c Ph e 235 ggt O31y tt g Le u ccg Pr o gcg Al a g at As p aac As n t ac Tyr ggt O31y 140 cgt Ar g ac g Thr gt g ValI t ca Ser at c I Ie 220 ag a Ar g ggg O31y ggc O31y gga O31y t gg Tr p tt c Ph e gcc Al a ac c Thr 125 cgc Ar g ccg Pr a ccg Pr a gca Al a ggt O31y 205 gga O31y t ct Ser agc ggt Ser 03 y caa gca O3 n Al a caa gac O31n As p t cg acc Ser Thr aac ccg As n Pr o ggt t gg O31y Tr p t tc at g Phe I'b t gac gcg As p Al a ct c gcc Leu Al a gaa caa O31u (1n 1 t cc ggc Ser 03 y 1 gcg acc Al a Thr ggt ggg O31y (1y at c gt g I e Val aag aaa Lys Lys 240 aag aac t aggt gt t ct t ct t cacgac ct c Page 117 ^Q seql i st cor r ect ed. t xt O Lys Asn ,C <210> 92 d) <211> 242 4 <212> PRT <213> Cor ynebact eri um gl ut an cum <400> 92 G n Tyr Thr Val Asp G n Leu Leu His G y Leu Leu Leu Ala Ser G y 1 5 10 0 Asn Asp Ala Ala Tyr Leu Leu Al a Gn u Leu G y y Asp n Ala 00 20 25 0 0 Thr Leu G u Lys Val Asn Ala Leu Ala Lys G u Leu G y Thr G n Asp (C 35 40 IO SThr Phe Val Ala Thr Tyr Ser G y Leu Asp Ala Pro G y IVet Ser Thr 55 Ser Ala Tyr Asp IVet Ser Leu Ile Tyr G n His Ala Trp n Asn Pro 70 75 Val Phe G u Ser IIe I e Ser Thr Asp His IIe Asp Phe Pro G y Trp 90 G y Asp Asn G u G y Phe G n Val Trp Asn Asp Asn Al a Leu Phe Iet 100 105 110 Asn Asp Pro Asp y Ile y y Lys Thr y Tyr Thr Asp Asp Ala 115 120 125 Asn His Thr Phe Val G y G y Leu Asp Arg G y G y Arg Arg Leu Ala 130 135 140 Ala Val Leu Leu Asp Ser Thr Val Ser Asp lie Arg Pro Trp G u G n 145 150 155 160 Ala Arg Leu Leu Ile Asp Ala Ser Leu Pro lie Thr Pro y Ser y 165 170 175 Val y G n Leu y Ser y Ser Ala Asn Asp Val Ala Pro Ala Thr 180 185 190 Pro G u Leu Pro G u Pro Thr Asp Asn Leu Thr Ser G y G u G y G y 195 200 205 Ser G n Asn Thr Leu Leu Lys Leu Val Val Pro Ile y Ile Ile Val 210 215 220 Leu Leu Leu lie Ala Ala Leu Ala Trp Thr Phe Arg Ser Pro Lys Lys 225 230 235 240 Lys Asn <210> 93 <211> 1212 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1189) <223> RXA00137 Page 118 seqi i st car r ect ed. t xt <400> 93 tttacagagt gcttatgagg caatcagcca ctaagtgttg agtaatctac tagtttggac t agaagt tac ccact t tcag t gaat tt t ta aggagagaac at g gct t tg gct N/t Al a Leu Al a ac c Thr c aa O31n ct c Le u ct c Le u g aa (31u ac g Thr gca Al a g aa O31u ct g Le u ct g Le u 1 50 g aa O31u cgc Ar g gga O31y c ag O31n t tc Ph e 230 tt t Ph e at c I Ie gga O31y gcg Al a gt c ValI ct a Le u ac c Thr gt c ValI 120 aaa Ly s t cc Ser cga Ar g tt g Le u aac As n 200 ggc O31y t cc Ser act Thr t ca Ser ac c Thr at c I Ie g ac As p c ag O31n ct g Le u ct g Le u ag c Ser gca Al a gag O31u 1 70 gca Al a gcg Al a ct a Le u at g vb t cgt Ar g at t G31y t gc CY s at c lIe 75 gt c ValI gac As p at c I Ie t t c Ph e t t c Ph e 155 cgc Ar g gaa O31u aaa Ly s gt c ValI acc Thr 235 cgt Ar g tt g Le u t cc Ser ac c Thr gag G u g aa (3u c ag O3n ccc Pr o g aa O3u 140 g aa O3u c aa O3n cgc Ar g cca Pr o ac c Thr 220 cgc Ar g cgc Ar g gt g ValI aac As n 45 g ac As p gcg Al a ggg O31y ct a Le u gt g ValI 125 ct c Le u g at As p gt c ValI ccc Pr o c ac Hi s 205 at c I Ie ac c Thr gca Al a ac a Thr ggc O31y ggt O31y ct g Le u ccg Pr o g ac As p 110 t ca Ser g ac As p tt a Le u gcc Al a ag c Ser 1 90 c ac Hi s g at As p ct c Le u gcc Al a ccg Pr a ct g Le u t ac Tyr gag G u 80 cgt Ar g ct g Le u gt t ValI ct c Le u gca Al a 1 60 g at As p g ac As p gca Al a ggc O3y at g vb t 240 1 gca Al a at c I Ie at c I Ie ac c Thr cgt Ar g at a I Ie cgt Ar g gt g ValI cgc Ar g 145 gca Al a ct g Le u ac c Thr ggc O31y gca Al a 225 ggc O31y aaa Ly s cat Hi s gt g ValI ac c Thr gcc Al a gca Al a g aa O31u g aa O31u 130 g at As p gga O31y g aa O31u at c I Ie g ac As p 210 c ac Hi s g aa O31u ct g Le u gt t ValI aac As n c ag O31n t cg Ser at c I Ie gca Al a 115 t cc Ser gt c ValI g aa O31u t ac Tyr gt g ValI 1 cgc Ar g gca Al a gca Al a gca Al a cgc Ar g aaa Ly s at c I Ie gca Al a g aa O31u 1 00 ac c Thr at t I Ie gca Al a ct c Le u cgc Ar g 1 gcc Al a at c I Ie cgc Ar g ggg (31y g at As p gct Al a t at Tyr g at As p gca Al a ac g Thr gcc Al a c ag O31n cgc Ar g gcg Al a gcc Al a 1 at g vb t t ct Ser ct c Le u gga O31y gag O31u 245 Page 119 NO seql i st cor r ect ed. t xt ttc gaa gca gaa atc tac gac atc gtc ctg cgc tcc caa ctc gct ggt 883 Phe u Ala u lie Tyr Asp lie Val Leu Arg Ser G n Leu Ala y 250 255 260 C gtt gaa gca gcc tac tca ggc gcc aac ctc ttc gac atc gac gca gca 931 Val u Al a Al a Tyr Ser G y Al a Asn Leu Phe Asp I e Asp Al a Al a S265 270 275 Stgc cgc aaa atc atc gaa gac gca ggc tac ggc gaa tac ttc gtg cac 979 Cys Arg Lys lie lie G u Asp Ala G y Tyr G y G u Tyr Phe Val His 280 285 290 C t1 tcc acc ggc cac ggc atc gga ctt gaa gtc cac gaa gcc cca agc gca 1027 S Ser Thr G y Hi s y Ile G y Leu G u Val His G u Ala Pro Ser Ala 00 295 300 305 0 tcc aaa acc tca caa gga gtc cta gaa acc ggc tcc aca ctg acc atc 1075 CK Ser Lys Thr Ser n G y Val Leu G u Thr y Ser Thr Leu Thr lie \C 310 315 320 325 0 gaa ccc gga att tac gtc ccc gga aag ggc ggc gta cgc atc gaa gac 1123 C 1 Gu Pro Oy Ile Tyr Val Pro y Lys G y y Val Arg Ile u Asp 330 335 340 acc ctg att att acc tca gga gca ccg gaa atc atc acc aag gtg agt 1171 Thr Leu lie lle Thr Ser G y Ala Pro Gu lie lle Thr Lys Val Ser 345 350 355 aag gac ctc atc gtg gtg taatctaggt gagctaatcg gtc 1212 Lys Asp Leu lie Val Val 360 <210> 94 <211> 363 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 94 IVet Ala Leu Ala Asp Thr Arg Phe Ala Thr Arg Arg Arg Ala Leu Ala 1 5 10 Ala Lys Leu Ala Ala G n Arg Ile Asp Ser Ile Leu Val Thr Ser Pro 25 I Ie His Val Arg Tyr Leu Ser G y Phe Thr G y Ser Asn G y Ala Leu 40 lie Val Asn Lys Asp Leu Ser Ala n lie Cys Thr Asp y Arg Tyr 55 Thr Thr n Ile Ala G u u Val Pro Asp ie G u Ala Leu Ile u 70 75 Arg Ala Ser Ala Thr Thr Leu Leu Ala G n Val G u y Pro Arg Arg 90 Ile Ala lle u Ala Ala n Thr Thr Leu Asp G n Leu Asp Ser Leu 100 105 110 Arg u Ala Thr n u Asp Val u Leu Ile Pro Val Ser y Val 115 120 125 Val u Ser lie Arg Leu Thr Lys Asp Ser Phe G u Leu Asp Arg Leu 130 135 140 Arg Asp Val Ala Ala Leu Ala Ser G n Ala Phe G u Asp Leu Leu Ala 145 150 155 160 Page 120 seql i st cor r ect ed. t xt Al a Gy u Leu Al a 165 G u G y Arg Ser Su Arg n Val Ala Ala Asp 170 175 Leu Thr Gy Al a 225 Gy Ser Asp G u G u 305 Ser Val SIle Tyr Val 195 Ar g Al a Al a Leu Asp 275 Phe Pr o Leu SIle Lys 355 bet Ser Leu Gy G u 245 Gy Al a Hi s Al a SIle 325 Asp Ser Leu Pr o Ar g 215 Asn G u G u Ar g Thr 295 Lys Pr o Leu Asp Leu Asn 200 Gy Ser Al a Al a Lys 280 Gy Thr Gy SIle Leu 360 Gy 185 Ser Asp Asp G u Al a 265 I le Hi s Ser I e I e 345 I le Al a Al a Leu Met SIle 250 Tyr SIle Gy G n Tyr 330 Thr Val G u Lys Val Thr 235 Tyr Ser G u SIle Gy 315 Val Ser Val Pr o Hi s 205 I le Thr SIle Al a Al a 285 Leu Leu Gy Al a Phe Gy Phe Val Leu 255 Leu Tyr Val Thr Gy 335 G u <210> <211> 1404 <212> DNA <213> Cor ynebact er i um gl ut arri cum <220> <221> CDS <222> (101)..(1381) <223> RXN00499 <400> tgccaacagg ggatatgcca ctgtgtaccc cacggcgatg atcggtaaaa tcctcggcgc gcagatattg ttcttgctgc tctaaggtga tttttgggca gtg gtg ggg gtg gtg Val Val G y Val Val 1 tcc acc cct gcg cgt aac ctg gga agc atg act aaa aca ctt ggt tcc Ser Thr Pro Ala Arg Asn Leu Gy Ser IVet Thr Lys Thr Leu Gy Ser 15 ctt cag ctg gaa gaa atc acg ctg acc ctc cct ctg act gaa gat gtg Leu G n Leu G u G u Ile Thr Leu Thr Leu Pro Leu Thr G u Asp Val 30 gcc gat gaa cgc acc att gat gtg ttc gca cgc att gcc aca cgc gtc Ala Asp G u Arg Thr Ile Asp Val Phe Ala Arg lie Ala Thr Arg Val Page 121 seql i st cor r ect ed. t xt ggt Gy aat Asn ggc Gy acc Thr gca Al a cga Ar g ctt Leu 150 cgg Ar g gct Al a at g Met gaa G u ctt Leu 230 cac Hi s gaa G u ttg Leu tct Ser cca Pr o gaa G u gct Al a gcc Al a cgt Ar g gt a Val 120 gct Al a ggc Gy gcc Al a gat Asp cga Ar g 200 ttc Phe acc Thr ttg Leu gat Asp ttc Phe 280 gcc Al a gat Asp ctt Leu cgt Ar g gag G u acc Thr gag G u gcc Al a t cc Ser t cc Ser 170 cca Pr o tct Ser ggg Gy gaa G u agc Ser 250 acc Thr aac Asn ggt Gy cgc Ar g cca Pr o cca Pr o 75 gaa G u cca Pr o tac Tyr ct g Leu tt c Phe 155 tt g Leu gca Al a gag G u tt g Leu gtt Val 235 aat Asn t cc Ser cgc Ar g gt g Val gag G u t at Tyr agc Ser t ac Tyr gt g Val tta Leu cgt Ar g 140 gga Gy gac Asp gaa G u gaa G u gtt Val 220 gt g Val gac Asp aac Asn aac Asn gt g Val 300 gat Asp tta Leu ctt Leu cgc Ar g ggt Gy t cc Ser 125 aag Lys ggt Gy aac Asn gac Asp ttc Phe 205 aat Asn t ca Ser ggc Gy gct Al a cca Pr o 285 aca Thr cca Pr o gt a Val aat Asn gt g Val aat Asn 110 cac Hi s cat Hi s ttc Phe gt g Val gt g Val 190 t ac Tyr cgt Ar g gaa G u tgg Tr p ttt Phe 270 at t SIle aat Asn aca Thr ct g Leu ct c Leu 80 at g Met at t SIle cgc Ar g ggt Gy acc Thr 160 at t SIle gcc Al a cgc Ar g cgc Ar g agg Ar g 240 gat Asp cat Hi s t ac Tyr gca Al a cag G n aac Asn ttg Leu ttg Leu gca Al a gt g Val 145 ct g Leu acc Thr aac Asn ttc Phe gcc Al a 225 ct g Leu ct g Leu gac Asp gt g Val gca Al a 305 ggt Gy ccc Pr o gat Asp gaa G u gat Asp 130 aat Asn cat Hi s ggc Gy tgt Cys ccg Pr o 210 ggg Gy cga Ar g t ac Tyr ct g Leu ct c Leu 290 gag G u ggg Gy aat Asn caa G n aaa Lys 115 ggc Gy cag G n t ac Tyr ggt Gy t ac Tyr 195 caa G n gag G u t cc Ser aac Asn gca Al a 275 cat Hi s cgt Ar g cct Pr o tgg Tr p cgt Ar g 100 ccc Pr o at t SIle tgg Tr p ttg Leu ct c Leu 180 aac Asn tta Leu at t SIle ctt Leu ct g Leu 260 gga Sy gag G u gt g Val ggc G y ttg Leu ggc Sy aca Thr gt g Val aac Asn t cc Ser 165 agc Ser cgc Ar g cgg Ar g gt g Val ggt Sy 245 ct g Leu ctt Leu t cc Ser ctt Leu 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 ctg ctc acc Leu Leu Thr Page 122 ggt gag cac gtg G y G u Hi s Val INO seql i st cor r ect ed. t xt 310 315 320 325 Sttc cag gag tgg aca gac acc gtg ccg tcg ctc aag ccg tgg aag gac 1123 Phe n G u Trp Thr Asp Thr Val Pro Ser Leu Lys Pro Trp Lys Asp 330 335 340 d) 4 gtt gcc ctg gca ttg gct cag cag gaa tgg ccc aag ctt tat gat gcg 1171 .Val Ala Leu Ala Leu Al a On On u Trp Pro Lys Leu Tyr Asp Ala 345 350 355 aag gca ttg gaa aac tca cag gcc aag ggc gct gca gca gtg tat gcc 1219 Lys Ala Leu G u Asn Ser G n Ala Lys y Ala Ala Ala Val Tyr Ala C1 360 365 370 00 aat gac gtt ttc gtc cca gtg gat tac tct ctg gaa acc gca caa cac 1267 0 Asn Asp Val Phe Val Pro Val Asp Tyr Ser Leu G u Thr Ala G n His 375 380 385 (N ctg ccc ggt gtg cag ctg ttt atc acc agc cag cat gaa cac aat gga 1315 SLeu Pro G y Val G n Leu Phe Ile Thr Ser G n Hi s u His Asn G y S390 395 400 405 (N ctt cgt gcc agc tca ggc gca gta ctg aag cac ctt ttc gat ctg gcc 1363 Leu Arg Ala Ser Ser G y Ala Val Leu Lys His Leu Phe Asp Leu Ala 410 415 420 cac ggc cga gag gta cgc tgattcctcg tgttagtact agc 1404 His G y Arg G u Val Arg 425 <210> 96 <211> 427 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 96 Val Val y Val Val Ser Thr Pro Ala Arg Asn Leu y Ser bet Thr 1 5 10 Lys Thr Leu y Ser Leu G n Leu G u G u lie Thr Leu Thr Leu Pro 25 Leu Thr G u Asp Val Ala Asp G u Arg Thr Ile Asp Val Phe Ala Arg 40 lie Ala Thr Arg Val G y y G u Asp Leu Pro Tyr Leu Val Phe Leu 55 Sn Gy y Pro y Asn G u Ala Pro Arg Pro Ser Leu Asn Pro Leu 70 75 Asn Pro Asn Trp Leu G y Val Ala Leu G u G u Tyr Arg Val Val IVet 90 Leu Asp G n Arg y Thr y Arg Ser Thr Pro Val y Asn Asp Ile 100 105 110 Leu G u Lys Pro Thr Al a G u Val Val G u Tyr Leu Ser His Leu Arg 115 120 125 Ala Asp G y IIe Val Arg Asp Ala G u Ala Leu Arg Lys His Leu G y 130 135 140 Val Asn G n Trp Asn Leu Leu y G n Ser Phe y y Phe Thr Thr 145 150 155 160 Leu His Tyr Leu Ser Arg His Ala Asp Ser Leu Asp Asn Val Phe Ile Page 123 seql i st cor r ect ed. t xt 165 Ser Ala lie Asp Thr Asn Phe Al a 225 Leu Leu Asp Val Al a 305 Thr Lys Lys Al a G u 385 Hi s Leu Gy Tyr 195 G n G u Ser Asn Al a 275 Hi s Ar g G u Tr p Tyr 355 Val Al a Hi s Asp Ar g Ar g Val Gy 245 Leu Leu Ser Leu Val 325 Asp Al a Al a Hi s Gy 405 Al a Ar g Thr 215 Pr o Leu Leu Pr o Tyr 295 G u G n Al a Al a Asp 375 Pr o Ar g Gy Ar g 200 Phe Thr Leu Asp Phe 280 Al a Asp G u Leu Leu 360 Val Gy Al a Ar g Ar g Pr o Al a 185 Asn Ser G u Arg G y Leu G y G u Val 235 G y Ser Asn 250 Pro Thr Ser 265 G y Asn Ar g Asp G y Val Phe Arg G u 315 Trp Thr Asp 330 Al a Leu Al a 345 G u Asn Ser Phe Val Pr o Val G n Leu 395 Ser Ser G y 410 Su Val Arg 425 Su Asp G u Phe 205 Val Asn 220 Val Ser Asp y Asn Al a Asn Pro 285 Val Thr 300 Asp Pro Thr Val G n n G n Al a 365 Val Asp 380 Phe Il e Al a Val Tyr Ar g Al a Thr Phe 255 Val Tyr Tr p Leu Ser 335 Tr p Gy Ser Ser Lys 415 Al a Ar g Ar g Ar g 240 Asp Hi s Tyr Al a Leu 320 Leu Pr o Al a Leu G n 400 Hi s <210> 97 <211> 980 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> <223> FRXA00499 <400> 97 ggt aat gat att ttg gaa aaa ccc aca gca gaa gta gtg gag tac tta Sy Asn Asp Ile Leu u Lys Pro Thr Ala G u Val Val u Tyr Leu 1 5 10 tcc cac ctg cgc gca gat ggc att gtg cga gat gct gaa gcc ctg cgt Ser His Leu Arg Ala Asp y IIe Val Arg Asp Ala G u Ala Leu Arg Page 124 I seql i st cor r ect ed. t xt 20 25 0 aag cat ttg ggt gtg aat cag tgg aac ctt tta ggc cag tcc ttc gga 144 Lys His Leu O y Val Asn 3 n Trp Asn Leu Leu O y O n Ser Phe O y 40 4 ggt ttc acc acc ctg cat tac ttg tcc cgg cac gcc gat tcc ttg gac 192 (3 y Phe Thr Thr Leu His Tyr Leu Ser Arg His Ala Asp Ser Leu Asp 55 aac gtg ttt att acc ggc ggt ctc agc gct att gat cgc cca gca gaa 240 Asn Val Phe Ile Thr y y Leu Ser Ala I le Asp Arg Pro Ala u c1 65 70 75 00 gac gtg tat gcc aac tgt tac aac cgc atg cgc cga aac tct gag gaa 288 SAsp Val Tyr Ala Asn Cys Tyr Asn Arg I t Arg Arg Asn Ser u u 90 C ttc tac cgt cgc ttc ccg caa tta cgg gaa act ttc cga ggg ttg gtt 336 SPhe Tyr Arg Arg Phe Pro n Leu Arg u Thr Phe Arg y Leu Val 100 105 110 aat cgt gct cgc gcc ggg gag att gtg ctt ccc acc ggc gaa gtt gtg 384 Asn Arg Ala Arg Ala y u Ile Val Leu Pro Thr y u Val Val 115 120 125 tca gaa acc agg ctg cga tcc ctt ggt cac ttg ttg ggt agc aat gac 432 Ser O u Thr Arg Leu Arg Ser Leu O y His Leu Leu O y Ser Asn Asp 130 135 140 ggc tgg ttt gat ctg tac aac ctg ctg gaa tta gat ccc acc tcc aac 480 Sy Trp Phe Asp Leu Tyr Asn Leu Leu u Leu Asp Pro Thr Ser Asn 145 150 155 160 gct ttt gtc cat gac ctg gca gga ctt ttg cct ttc ggc aac cgc aac 528 Ala Phe Val His Asp Leu Ala O y Leu Leu Pro Phe O y Asn Arg Asn 165 170 175 cca att tat tac gtg ctc cat gag tcc tct tac gcc gac ggt gtg gtg 576 Pro Ile Tyr Tyr Val Leu His u Ser Ser Tyr Ala Asp O y Val Val 180 185 190 aca aat tgg gca gca gag cgt gtg ctt cca gag gat ttc cgc gag gat 624 Thr Asn Trp Ala Ala u Arg Val Leu Pro u Asp Phe Arg u Asp 195 200 205 cca aca ctg ctc acc ggt gag cac gtg ttc cag gag tgg aca gac acc 672 Pro Thr Leu Leu Thr G y u His Val Phe n O u Trp Thr Asp Thr 210 215 220 gtg ccg tcg ctc aag ccg tgg aag gac gtt gcc ctg gca ttg gct cag 720 Val Pro Ser Leu Lys Pro Trp Lys Asp Val Ala Leu Ala Leu Ala n 225 230 235 240 cag gaa tgg ccc aag ctt tat gat gcg aag gca ttg gaa aac tca cag 768 n O u Trp Pro Lys Leu Tyr Asp Ala Lys Ala Leu u Asn Ser n 245 250 255 gcc aag ggc gct gca gca gtg tat gcc aat gac gtt ttc gtc cca gtg 816 Ala Lys O y Ala Ala Ala Val Tyr Ala Asn Asp Val Phe Val Pro Val 260 265 270 gat tac tct ctg gaa acc gca caa cac ctg ccc ggt gtg cag ctg ttt 864 Asp Tyr Ser Leu O u Thr Ala O n His Leu Pro O y Val O n Leu Phe 275 280 285 atc acc agc cag cat gaa cac aat gga ctt cgt gcc agc tca ggc gca 912 Ile Thr Ser O n His O u His Asn O y Leu Arg Ala Ser Ser O y Ala Page 125 seqi i st car r ect ed. t xt 290 295 300 gt a ct g aag cac ct t t tc gat ctg gcc cac ggc cga gag gta cgc Val Leu Lys His Leu Phe Asp Leu Ala His Gy Arg G31u ValI Arg 305 310 315 t gat tcct cg t gt tagt act agc <210> 98 <211> 319 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 98 31y As n Ser Hi s Lys Hi s G3 y Phe As n Val As p Val Phe Tyr Asn Arg Ser G3 u 130 G31y Tr p 145 A a Phe Pr o I Ie Thr As n Pr o Thr 210 Val Pr o 225 (31n (1u A a Lys Asp Tyr Asp I I e Leu Arg Leu G3 y Thr Thr Phe I I e Ty r A a Ar g Ar g 1 00 Ala Ar g 115 Thr Ar g Phe As p Val Hi s Tyr Tyr 1 80 Tr p Ala 1 95 Leu Leu Ser Leu Tr p Pr o (3 y A a 260 Ser Leu 275 Le u A a ValI Le u Thr As n Ph e A a Le u Le u As p 1 65 ValI A a Thr Lys5 Lys5 245 A a (31u G u Lys Pro Thr As p As n Hi s a31y 70 Cy s Pr o a31y Ar g Tyr 150 Le u Le u a31u a31y Pr o 230 Le u A a Thr a31y a31n Tyr 55 a31y Tyr a31n a31u Ser 135 As n A a Hi s Ar g a31u 215 Tr p Tyr ValI A a ValI 25 As n Ser Ser Ar g Ar g 1 05 ValI a31y Le u Le u Ser 1 85 Le u ValI As p A a A a 265 Hi s A a 10 Ar g Le u Ar g A a vb t 90 a31u Le u Hi s a31u Le u 1 70 Ser Pr o Ph e ValI Lys5 250 As n a31u As p Le u Hi s I Ie Ar g Thr Pr a Le u Le u 1 Pr a Tyr a31u a31n A a 235 A a As p ValI A a a31y A a As p Ar g Ph e Thr Le u 140 As p Ph e A a As p a31u 220 Le u Le u ValI ValI a31u a31n As p Ar g As n Ar g a31y 125 a31y Pr a a31y As p Ph e 205 Tr p A a a31u Ph e a31u A a Ser Ser Pr a Ser a31y 110 a31u Ser Thr As n a31y 1 Ar g Thr Le u As n ValI 270 Tyr Le u Ph e Le u A a a31u Le u ValI As n Ser Ar g 1 ValI a31u As p A a Ser 255 Pr a Leu Pro (3y Val (31n Leu Phe 285 Page 126 seqi i st car r ect ed. t xt I Ie Th r Se r (31n Hi s O31u Hi s As n G31y L eu Ar g Ala Se r Se r G31y Ala 290 295 300 Val Leu Lys His Leu Phe Asp Leu Ala His Gy Arg O31u VaI Ar g 305 310 315 <210> 99 <211> 1788 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CDS <222> (1765) <223> RXN00877 <400> 99 aaat aat ggg gct cgccggt gat ggt tccc gccgggcat t caacggt gac ggaagaggt g gcagacat ga t gaaaact ct agcaact agt at cggt cact at g act gt t gaa cac l'bt Thr Val O31u Hi s ct g Le u at c I Ie c ac Hi s t gg Tr p cgc Ar g g aa O31u t cg Ser gt a ValI aag gcc A a 1 50 tt c Ph e aag Lys5 gt g ValI gct A a aac As n gcc A a g at As p gcg A a gca A a 120 gct A a ct g Le u cgc Ar g ag c Ser g at As p at t I Ie at t I Ie gt a ValI at c I Ie tt c Ph e gcc A a cga Ar g ac c Thr ct g Le u 1 70 tt g Le u ccg Pr o gcg A a gcc A a tt c Ph e gcc A a aat As n gag O31u cgc Ar g 140 aac As n c ag (31n ccc Pr o ccc Pr o at c I Ie 45 ct g Le u aac As n act Thr gct A a g aa O31u 125 ggt G31y c ag O31n g ac As p t ac Tyr gcc A a gct A a g aa O31u tt g Le u at c I Ie gcg A a 110 t cg Ser gca A a cgc Ar g ac c Thr c ag O31n 15 tt c Ph e ac c Thr cgc Ar g c ag O31n gcg A a 95 ct t Le u c aa O31n gca A a ct a Le u cgc Ar g 1 75 ct g Le u g aa O31u aat As n gca A a ggc O31y ccg Pr o tt c Ph e cgc Ar g ct c Le u t cg Ser 1 g at As p 1 ccc Pr a ct c Le u gag O31u O31y ac c Thr aaa Lys5 gcg A a ct g Le u aac As n 145 gca A a ct g Le u g at As p gca A a g ac As p ct g Le u g at As p ct c Le u cgc Ar g tt g Le u 130 gcc A a ct g Le u gcg A a tt c Ph e tt a Le u gct A a t cc Ser t cc Ser t cc Ser at t I Ie 115 t cc Ser g ac As p t cc Ser gt c ValI gca A a gct A a cct Pr a ct c Le u t cc Ser gcg A a 1 00 gag O31u c ac Hi s 99c O31y g aa O31u aac As n 1 Page 127 seql i st cor r ect ed. t xt gaa G u gct Al a gaa G u tcg Ser 230 aac Asn aca Thr acc Thr cca Pr o gaa G u 310 tgg Tr p ct g Leu ttc Phe ct g Leu gac Asp 390 t cc Ser ct g Leu cca Pr o tct Ser t ac Tyr 200 ccc Pr o gca Al a gac Asp tta Leu gat Asp 280 gcc Al a gaa G u gcc Al a aac Asn gct Al a 360 ggt Sy t cc Ser cgg Ar g ggc G y acc Thr 440 ctt gcc ggt ttt Leu Al a y Phe agc gaa gcc gca Al a gt g Val ctt Leu ct g Leu 250 t ac Tyr gt c Val gcc Al a cac Hi s gt c Val 330 ttc Phe cgc Ar g gcc Al a at c SIle ggc G y 410 aag Lys gaa Gu gca Al a cag G n t at Tyr 235 ct c Leu gac Asp gca Al a aat Asn ggc Gy 315 cgc Ar g cca Pr o ct c Leu gag G u ggc G y 395 gct Al a cca Pr o gca Al a gtt Val t ca Ser 220 gaa G u gaa G u acc Thr gcc Al a gcg Al a 300 caa G n gcc Al a ttg Leu t ac Tyr ggc G y 380 ct g Leu tgg Tr p gt c Val ct a Leu ggc G y 205 gag G u gcc Al a acc Thr cac Hi s gt g Val 285 aaa Lys aaa Lys cgc Ar g aac Asn gga G y 365 gt g Val at c SIle at g Met gt g Val ct c Leu 445 Ser 190 acc Thr cag G n t cc Ser gt g Val gcc Al a 270 cgc Ar g gcc Al a gt c Val gac Asp caa G n 350 at c SIle gac Asp ctt Leu t cc Ser gt c Val 430 agc Ser G u Al a gaa G u gca Al a cag G n cgt Ar g 255 gat Asp gcc Al a gaa G u ggc G y t ac Tyr 335 gt a Val acc Thr gt c Val acc Thr agc Ser 415 aac Asn ct c Leu ggc G y gt a Val aag Lys 240 ct g Leu t ac Tyr ttg Leu t ac Tyr gca Al a 320 gcc Al a ct c Leu gt g Val tgg Tr p gac Asp 400 ttt Phe gtt Val gat Asp cgc at a t cc Arg Ile Ser 195 tac gtg gtt Tyr Val Val 210 tta acc gaa Leu Thr G u 225 cgt ggc gcc Ar g G y Al a cgt gct gaa Ar g Al a G u gt c at c gaa Val Ile G u 275 ctt tat gat Leu Tyr Asp 290 aaa ct c t cc Lys Leu Ser 305 gct gac tgg Ala Asp Trp ctg gac gaa Leu Asp G u cgt gac ggc Arg Asp G y 355 gaa cca cgc G u Pro Arg 370 gaa gt c ct c G u Val Leu 385 tac tac ggc Tyr Tyr G y gtc gac caa Val Asp G n atg ggt att IVet G y lle 435 gaa gta acc G u Val Thr 450 gcc Al a cca Pr o t cc Ser agc Ser cgc Ar g 260 gaa G u ct c Leu gca Al a agc Ser acc Thr 340 gt c Val cct Pr o gat Asp cga Ar g t cc Ser 420 acc Thr acc Thr gcc Al a ct g Leu gcc Al a ct g Leu 245 gcc Al a gaa G u gcc Al a gaa G u ttc Phe 325 gaa G u ttc Phe gac Asp t ct Ser cca Pr o 405 gag G u aaa Lys at c SIe 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 Page 128 sec ttc cac gaa ttc ggc cac ggc ctg c Phe Hi s G u Phe G y Hi s G y Leu H 455 460 tac cca agc ttc tcc gga acc tcc g Tyr Pro Ser Phe Ser G y Thr Ser 470 475 ccc tcc cag atc aac gaa aac tgg g Pro Ser Gn Ile Asn G u Asn Trp A 490 aac tac gcc cgc cac gtg gac acc g Asn Tyr Ala Arg His Val Asp Thr C 505 5 ctt gag gca gtg gaa gca tgt ggc a Leu u Ala Val u Ala Cys y I 520 525 gtg agt act tgt ccc cat cta tta t Val Ser Thr Cys Pro His Leu Leu S 535 540 cca cag cgg atg ccg cac tagtcaatg Pro G n Arg IVet Pro His 550 555 <210> 100 <211> 555 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 100 bet Thr Val G u His Leu Leu Lys F 1 5 Pro Asp Phe Ala Ala l e Lys Val A Leu Ala Leu Ala G u His Asp Ala C 40 G u Asp Ala Pro Thr Trp G u Asn T 55 Sy Leu Ser Leu Asn Arg Val Ala A 70 Thr Asp Ser Ser Pro G u IVet Asp C Lys Leu Ser Ala His Ser Asp Ala I 100 1 Ala Arg Ile u Ala Val u Ala F 115 120 Leu Leu Ser His Thr Lys Arg Ala F 130 135 Asn Ala Asp y Lys Al a Arg Leu S 145 150 Al a Leu Ser G u G n Phe G y Arg A 165 1 i st cor r ect ed. t xt ac ggc ttg ctg tcc i s G y Leu Leu Ser 465 itg ccc cgc gac tac (al Pro Arg Asp Tyr 480 ca ttc gac cct gca la Phe Asp Pro Ala 495 gc gac atc att cca Sy Asp Ile Ile Pro 10 tt tca gac aga gtg le Ser Asp Arg Val 530 cg acc tgc cct gtc ier Thr Cys Pro Val 545 ia cat t gaccaa t t a aag Lys gt a Val gt a Val gac Asp 515 gt g Val t ct Ser Tyr Al a Al a G u Leu SIle Al a 110 Ser Al a Ar g gtg cgc Val Arg gaa ttc G u Phe 485 gtc cgc Val Arg 500 t cc ct g Ser Leu gaa cat Su Hi s ccc tgt Pro Cys G n Leu Phe u Thr Asn Arg Ala G n G y Ala Pro Leu Phe G n Arg Al a Leu Leu Ser 160 Arg Asp 175 1507 1555 1603 1651 1699 1747 1788 r o ~l a 25 Su Fhr ~l a Su le 05 r o 'he ier sn Ser 10 Asp SIle SIle Val SIle 90 Phe Al a Ar g Thr Thr Phe Thr G u Phe 75 Al a Hi s Asp Ar g SIle 155 Leu Pr o Al a Al a Phe Al a Asn G u Ar g 140 Asn Pr o Pr o l Ie Leu Asn Thr Al a G u 125 Sy G n Leu Leu G n Asp Thr 170 Page 129 seqi i st car r ect ed. t xt Leu Ala Val Asn Phe O31u (31u Ser O31u Leu Ala G31y Phe Ser O31u Ala 1 80 Ar g Tyr Le u 225 Ar g Ar g ValI Le u Ly s 305 A a Le u Ar g O31u O31u 385 Tyr ValI vb t O31u Le u 465 As p Pr o I Ie Ser 1 95 ValI O31u A a O31u O31u 275 As p Ser Tr p O31u G31y 355 Ar g Le u G31y O31n I Ie 435 Thr Ly s ValI ValI As p 515 A a Le u A a Le u 245 A a O31u A a (31u Ph e 325 O31u Ph e As p Ser Pr o 405 O31u Ly s I Ie Ar g Ph e 485 Ar g Le u A a O31u Ser 230 As n Thr Thr Pr o (31u 310 Tr p Le u Ph e Le u As p 390 Ser Le u Pr o Ph e Tyr 470 Pr a As n Le u As p Le u 215 Ar g Ly s Le u A a A a 295 A a O31u Ar g A a Ar g 375 G31y Ly s Le u Thr Hi s 455 Pr a Ser Tyr (31u Tyr 200 Pr a A a As p Le u As p 280 A a G u A a As n A a 360 G3y Ser Ar g O3y Thr 440 O3u Ser O3n A a A a 520 1 85 A a Thr Ly s ValI O31y 265 As p Ser (31u Ly s Tyr 345 As n Tyr O31y O31y Thr 425 O31y Ph e Ph e I Ie Ar g 505 ValI 1 A a ValI Le u Le u 250 Tyr ValI A a Hi s ValI 330 Ph e Ar g A a I Ie O31y 410 Ly s O31u O31y Ser As n 490 Hi s (31u A a O31n Tyr 235 Le u As p A a As n (31y 315 Ar g Pr a Le u O31u G31y 395 A a Pr a A a Hi s G31y 475 O31u ValI A a ValI Ser 220 O31u O31u Thr A a A a 300 (31n A a Le u Tyr G31y 380 Le u Tr p ValI Le u G31y 460 Thr As n As p Cys G31y 205 O31u A a Thr Hi s ValI 285 Ly s Ly s Ar g As n G31y 365 ValI I Ie vb t ValI Le u 445 Le u Ser Tr p Thr G31y 525 Thr O31n Ser ValI A a 270 Ar g A a ValI As p O31n 350 li1e As p Le u Ser ValI 430 Ser Hi s ValI A a O31y 510 li1e O31u A a O31n Ar g 255 As p A a O31u G31y Tyr 335 ValI Thr ValI Thr Ser 415 As n Le u O31y Pr a Ph e 495 As p Ser O31y ValI Ly s 240 Le u Tyr Le u Tyr A a 320 A a Le u ValI Tr p As p 400 Ph e ValI As p Le u Ar g 480 As p I Ie As p Ar g Val Val O31u Hi s Val Ser Thr Cys Pro Hi s Leu Leu Ser Thr Cys Page 130 INO seqI i st car r ect ed. t xt 530 535 540 Pr o Val Ser Pro Cys Pro On Arg I'bt Pro His C1 545 550 555 S<210> 101 <211> 1088 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> C1 <221> CIDS S<222> (1)..(1065) 00 <223> FRXA00877 S<400> 101 C1 gca gca gca gtt ggc acc gaa ggc tac gtg gtt cca ctg gaa ctg ccc 48 A% Aa Ala Ala Val G31y Thr O31u G31y Tyr Val Val Pr o Leu O31u Leu Pr o 1 5 10 C1 acc gtg cag tca gag cag gca gta tta acc gaa tcc gcc tcg cgt gca 96 Thr Val O31n Ser O31u O31n Ala Val Leu Thr O31u Ser Ala Ser Ar g Ala 25 aag ctt tat gaa gcc tcc cag aag cgt ggc gcc agc ctg aac aag gac 144 Lys Leu Tyr O31u Ala Ser O31n Lys Ar g G31y Ala Ser Leu Asn Lys Asp 40 gt g ct g ct c gaa acc gt g cgt ct g cgt gct gaa cgc gcc aca ct t t ta 192 Val Leu Leu 03 u Thr Val Ar g Leu Ar g Ala 03 u Ar g Ala Thr Leu Leu 55 ggc tac gac acc cac gcc gat tac gtc atc gaa gaa gaa acc gcc gat 240 G31y Tyr Asp Thr Hi s Ala Asp Tyr Val Ilie O31u 31u O31u Thr Ala Asp 70 75 gac gtc gca gcc gtg cgc gcc ttg ctt tat gat ctc gcc cca gcc gcc 288 Asp Val Ala Ala Val Ar g Ala Leu Leu Tyr Asp Leu Ala Pr o Ala Ala 90 tct gcc aat gcg aaa gcc gaa tac aaa ctc tcc gca gaa gaa gca gaa 336 Ser A a Asn A a Lys A a 03 u Tyr Lys Leu Ser A a 03 u 03 u A a 03 u 100 105 110 gaa cac ggc caa aaa gt c ggc gca gct gac t gg agc t tc t gg gaa gcc 384 O31u His G31y O31n Lys Val G31y Ala Ala Asp Tr p Ser Phe Tr p O31u Ala 115 120 125 aaa gt c cgc gcc cgc gac t ac gcc ct g gac gaa acc gaa ct g cgc aac 432 Lys Val Ar g Ala Ar g Asp Tyr Ala Leu Asp O31u Thr O31u Leu Ar g Asn 130 135 140 t act t cccat t gaac caa gt act ccgt gac ggc gt ctt ct tc gct gct 480 Tyr Phe Pr o Leu Asn O31n Val Leu Ar g Asp G31y Val Phe Phe Ala Ala 145 150 155 160 aac cgc ct C t ac gga at c acc gt g gaa cca cgc cct gac ct g cgc ggt 528 Asn Ar g Leu Tyr G31y Ilie Thr Val O31u Pr o Ar g Pr o Asp Leu Ar g G31y 165 170 175 t ac gcc gag ggc gt g gac gt c t gg gaa gt c ct c gat t ct gac ggc t cc 576 Tyr Ala 31u G31y Val Asp Val Tr p O31u Val Leu Asp Ser Asp G31y Ser 180 185 190 ggc atc ggc ctg atc ctt acc gac tac tac ggc cga cca tcc aag cgg 624 3y Ilie (3y Leu Ilie Leu Thr Asp Tyr Tyr C3y Ar g Pr o Ser Lys Ar g 195 200 205 Page 131 seql i st cor r ect ed. t xt ggc ggc gct tgg atg tcc agc ttt gtc gac Gy y Ala Trp [et Ser Ser Phe Val Asp 210 215 acc aag cca gtc gtg gtc aac gtt atg ggt Thr Lys Pro Val Val Val Asn Val MVt y 225 230 ggc gaa gca cta ctc agc ctc gat gaa gta Gy G u Ala Leu Leu Ser Leu Asp G u Val 245 250 ttc ggc cac ggc ctg cac ggc ttg ctg tcc Phe G y His G y Leu His G y Leu Leu Ser 260 265 ttc tcc gga acc tcc gtg ccc cgc gac tac Phe Ser Gy Thr Ser Val Pro Arg Asp Tyr 275 280 atc aac gaa aac tgg gca ttc gac cct gca Ile Asn G u Asn Trp Al a Phe Asp Pro Al a 290 295 cgc cac gtg gac acc ggc gac atc att cca Arg His Val Asp Thr y Asp lie lie Pro 305 310 gtg gaa gca tgt ggc att tca gac aga gtg Val G u Ala Cys y Ile Ser Asp Arg Val 325 330 tgt ccc cat cta tta tcg acc tgc cct gtc Cys Pro His Leu Leu Ser Thr Cys Pro Val 340 345 atg ccg cac tagtcaatga cattgaccaa tta IVet Pro His 355 <210> 102 <211> 355 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 102 Alaa Aa Ala Val y Thr Gu y Tyr Val 1 5 10 Thr Val G n Ser G u G n Ala Val Leu Thr Lys Leu Tyr G u Ala Ser G n Lys Arg y 40 Val Leu Leu G u Thr Val Arg Leu Arg Ala G y Tyr Asp Thr His Al a Asp Tyr Val lie 70 Asp Val Ala Ala Val Arg Ala Leu Leu Tyr 90 Ser Ala Asn Ala Lys Al a G u Tyr Lys Leu 100 105 Pag caa tcc gag ctg G n Ser G u Leu 220 att acc aaa cca Ile Thr Lys Pro 235 acc acc atc ttc Thr Thr lie Phe aag gtg cgc tac Lys Val Arg Tyr 270 gta gaa ttc ccc Val G u Phe Pro 285 gta gtc cgc aac Val Val Arg Asn 300 gac tcc ctg ctt Asp Ser Leu Leu 315 gtg gaa cat gtg Val G u His Val tct ccc tgt cca Ser Pro Cys Pro ct a Leu acc Thr cac Hi s 255 cca Pr o t cc Ser t ac Tyr gag agt Ser 335 cag G n Leu Ar g Lys Leu Al a Al a Al a ggc G y acc Thr 240 gaa G u agc Ser cag G n gcc Al a gca Al a 320 act Thr cgg Ar g 672 720 768 816 864 912 960 1008 1056 1088 Val I G u Al a G u G u 75 Asp I Ser e 132 Leu Al a Leu Al a a u Al a G u Pr o Al a Asp Leu Asp Al a G u seqi i st car r ect ed. t xt a3 u Hi s G3 y 115 On Lys Val Gy Ala Ala Asp Trp Ser 120 Phe Trp Ou Ala 125 Ly s Tyr 145 As n Tyr G31y G31y Thr 225 (31y Ph e Ph e I Ie Ar g 305 ValI cys b t Ar g Pr o Le u O31u G31y 1 95 Al a Pr o Al a Hi s G31y 275 O31u ValI Al a Hi s Hi s 355 Ar g As n G31y 1 65 ValI I Ie vb t ValI Le u 245 Le u Ser Tr p Thr G31y 325 Le u Tyr 135 ValI Thr ValI Thr Ser 215 As n Le u G31y Pr o Ph e 295 As p Ser Thr Al a Le u ValI Tr p As p 200 Ph e ValI As p Le u Ar g 280 As p I Ie As p Cys Le u Ar g O31u O31u 1 Tyr ValI vb t (31u Le u 265 As p Pr o I Ie Ar g Pr a 345 O31u G31y 1 Ar g Le u G31y O31n 235 Thr Ly s ValI ValI As p 315 ValI Ser O31u Ph e As p Ser Pr a 205 O31u Ly s I Ie Ar g Ph e 285 Ar g Le u Hi s Cys Ar g Al a Ar g 1 G31y Ly s Le u Thr Hi s 255 Pr a Ser Tyr O31u Ser 335 O31n <210O> 103 <21 1> 2724 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (2701) <223> RXN01014 <400> 103 t ct t aaagt t t t ct agcaat ccacact agg cgcgaact at cgt ggt gt ca t t gcgcacct t ct aagggt a gcgccccct c aaat t tcaag gagcat taaa t tg acg t cc act aat 115 Leu Thr Ser Thr Asn 1 ct c acc cga cag gaa gct t cg gat cgt t cg agg t ta ct g agt gt a gaa 163 Page 133 Leu Thr Arg On G u Ala Ser Asp eql i st cor r ect ed. t xt Arg Ser Arg Leu Leu aac Asn t cc Ser at t SIle t cc Ser acg Thr cgg Ar g cgc Ar g gag G u ct c Leu 150 at c SIle gat Asp gcg Al a acg Thr act Thr 230 gat Asp cac Hi s gac Asp acc Thr ct c Leu aaa Lys ggc Gy acg Thr gt g Val 120 gcc Al a gct Al a t cc Ser cac Hi s tgc Cys 200 acc Thr ccg Pr o gt g Val aac Asn gca Al a gt c Val gca Al a gag G u gcc Al a tct Ser cca Pr o gcc Al a t at Tyr tct Ser 170 t cc Ser ggt Gy cat Hi s gct Al a ct g Leu 250 ggt Gy ctt Leu agc Ser gca Al a gct Al a 75 ct g Leu at c SIle gca Al a aag Lys gat Asp 155 gag G u cga Ar g cgt Ar g gca Al a ct c Leu 235 ttt Phe gt g Val gat Asp ttc Phe agc Ser ct a Leu aag Lys ccc Pr o gac Asp cgt Ar g 140 ct g Leu cag G n gt g Val t ac Tyr gaa G u 220 t ac Tyr acc Thr gcg Al a aac Asn gt c Val gag G u ctt Leu ctt Leu t cc Ser 110 gag G u ttc Phe at c SIle gtt Val t at Tyr 190 gag G u cct Pr o cgc Ar g acg Thr cca Pr o 270 aac Asn agg Ar g gag G u ggc Gy act Thr 95 cgc Ar g gt g Val gcg Al a aaa Lys t cc Ser 175 ccc Pr o gt g Val gcc Al a agt Ser aag Lys 255 ttc Phe ggt G y aag Lys gtt Val aag Lys 80 ccc Pr o acc Thr t at Tyr tgt Cys act Thr 160 act Thr ct c Leu tgc Cys gat Asp tct Ser 240 cag G n ggc Gy gat Asp gct Al a cgc Ar g aac Asn ggc Gy ggt Gy ttg Leu ttc Phe 145 cct Pr o cag G n t cc Ser gat Asp cag G n 225 ttg Leu ggc G y aag Lys gag G u ggc G y ct g Leu ggc G y gcg Al a gaa G u t ac Tyr 130 gat Asp aag Lys cac Hi s acc Thr gt c Val 210 cca Pr o gct Al a ttt Phe t ac Tyr Ser Val G u ttt ttt agt Phe Phe Ser gat acc ttt Asp Thr Phe gac aat gtg Asp Asn Val tac gac gag Tyr Asp G u cac acc ttg Hi s Thr Leu 100 ggc ctg cac G y Leu Hi s 115 acc cag ttt Thr G n Phe cag cca gac Sn Pro Asp ggt t gg aag G y Trp Lys 165 act gat t ac Thr Asp Tyr 180 tac ctg att Tyr Leu Ile 195 tgg aag ggt Trp Lys G y act gag ct g Thr G u Leu aaa gat ctt Lys Asp Leu 245 gat tgg tac Asp Trp Tyr 260 gat cag at c Asp G n Ile ttc gtc cct gaa ttt aat gct ggc gcg atg gag aac gcc ggc gct gtc Page 134 Phe Val Pro Ou Phe Asn Ala 01 280 2 seqi i st car r ect ed. t xt y Ala l'bt Ou Asn Ala(3Oy Ala Val 5 290 ac c Thr t ac Tyr 310 t tc Ph e gag O31u g aa O31u gcg Al a gga O31y 390 gca Al a gag O31u ggc O31y ggc O31y at c I Ie 470 t cc Ser cgg Ar g ct c Le u ag c Ser cgc Ar g cgc Ar g gt g ValI tt c Ph e aac As n 360 c ag O31n g ac As p ggc O31y tt c Ph e gcc Al a 440 g ac As p ac c Thr t cc Ser c ac Hi s cgc Ar g 520 g aa O31u gag O31u gct Al a gt g ValI 330 act Thr gca Al a g at As p gag O31u t cg Ser 410 gca Al a tt t Ph e t cc Ser ggc O31y ac c Thr 490 at c I Ie gca Al a at c I Ie t ac Tyr gaa O31u 315 acc Thr t gg Tr p t gg Tr p cag O31n act Thr 395 gt g ValI O31y g at As p gac As p gca Al a 475 cag O31n gcg Al a cga Ar g gt t ValI gt t ValI 300 ac c Thr at g vb t t cc Ser gt g ValI ct g Le u 380 gt c ValI ct c Le u gt a ValI g at As p t gg Tr p 460 aag Ly s ac c Thr gt g ValI gt a ValI gga O31y 540 gca Al a ct t Le u t gg Tr p gca Al a 350 tt c Ph e t cc Ser c ag O31n c ag O31n ag g Ar g 430 ct c Le u aac As n ac c Thr gcc Al a ct t Le u 510 ct t Le u gag O31u t cc Ser c ac Hi s t gg Tr p 335 at t I Ie gcc Al a ac c Thr aac As n ct g Le u 415 c ac Hi s ggc O31y c ag O31n ac c Thr gcg Al a 495 t at Tyr g ac As p c ag O31n aag Ly s gag O31u 320 g at As p t ct Ser aat As n c ac Hi s tt c Ph e 400 c ag O31n tt t Ph e gcc Al a t gg Tr p g ac As p 480 ccg Pr o aag Ly s t gc cys gct Al a gca Al a 305 ct c Le u g at As p c ag O31n gt g ValI ccg Pr o 385 g ac As p gca Al a gcc Al a ct c Le u ct c Le u 465 aac As n ggt O31y ct t Le u agt Ser g ac As p 545 ac c Thr gct Al a ct g Le u gct Al a gag O31u 370 gt g ValI O31y t ac Tyr aac As n gag O31u 450 aag Ly s ggc O31y gcc Al a gt c ValI ggc O31y 530 tt c Ph e cgt Ar g c ac Hi s t gg Tr p gag O31u 355 aag Ly s tt c Ph e at c I Ie gt t ValI c ac Hi s 435 c ag O31n ac c Thr aaa Ly s ggt O31y g ac As p 515 gcg Al a gt g ValI t ac Tyr at g vb t ct c Le u 340 g aa O31u t cg Ser t ct Ser ac c Thr ggc O31y 420 gca Al a t cc Ser ac c Thr t ac Tyr gag O31u 500 gga O31y t cg Ser ct g Le u 1 027 1 075 1123 1171 1219 1267 1315 1363 1411 1459 1 507 1 555 1 603 1651 1 699 1 747 1 795 aac gat gat gat ct g acg t at gcg ct g ct g gat ct g gat gat gat t ca Page 135 seal i st cor r ect ed. t xt Asn Asp Asp Asp Leu Thr Tyr Al 550 555 a Leu Leu cgc Ar g cgc Ar g at g Met gaa G u gcg Al a 630 gac Asp gac Asp ct c Leu gt c Val gcg Al a 710 gct Al a cga Ar g aag Lys cac Hi s gcc Al a 790 ttc Phe ttt Phe ct g Leu gct Al a 600 gaa G u aag Lys ct g Leu gac Asp gat Asp 680 ggc G y at c l Ie ctt Leu ggt Gy gt c Val 760 at t SIle aac Asn ggc Gy at c SIle 570 tgg Tr p gat Asp gct Al a t ac Tyr gat Asp 650 cag G n gct Al a acc Thr cgt Ar g cgc Ar g 730 gct Al a gca Al a ggc G y cag G n at g Met 810 gac Asp t cc Ser tt c Phe gt g Val gcc Al a 635 gct Al a tt g Leu gcc Al a gt g Val ggt G y 715 gac Asp gt g Val gtt Val ct c Leu tac Tyr 795 gca Al a aat Asn gct Al a at c SIle ct g Leu 620 gac Asp ttc Phe gcg Al a gat Asp gat Asp 700 gac Asp aac Asn aac Asn gat Asp aca Thr 780 ttc Phe cag G n at t SIle gcg Al a gcg Al a 605 gag G u cca Pr a ctt Leu ttc Phe t ac Tyr 685 cct Pr a at c SIle t cc Ser act Thr agt Ser 765 ttc Phe gaa G u cag G n gat Asp tgg Tr p 590 ct g Leu cgc Ar g gcg Al a gag G u at t SIle 670 ttc Phe gac Asp gag G u agt Ser gaa G u 750 ggc G y act Thr at c SIle at c SIle aag Lys 575 gag G u gtt Val at t SIle tgg Tr p ggt G y 655 cag G n cgc Ar g ct g Leu gct Al a gcc Al a 735 gaa G u ct a Leu ggc G y ctt Leu gt c Val 815 Asp 560 ttc Phe at g Met gct Al a ct c Leu gca Al a 640 gct Al a gct Al a gac Asp cgt Ar g gt c Val 720 t cc Ser gt g Val t cc Ser tct Ser gat Asp 800 ct c Leu agc Ser act Thr cgt Ar g gcg Al a 625 gaa G u cgc Ar g ct g Leu at t SIle tgg Tr p 705 gaa G u ttc Phe aag Lys aac Asn tct Ser 785 gat Asp gga G y gac Asp cgc Ar g ggc G y 610 cag G n gca Al a t cc Ser gca Al a ctt Leu 690 tgg Tr p gat Asp ct c Leu gct Al a ct g Leu 770 gaa G u gt g Val ct g Leu cct Pr a gct Al a 595 gct Al a gct Al a act Thr gca Al a aaa Lys 675 gcc Al a gca Al a gca Al a gca Al a gct Al a 755 gag G u ct g Leu tgg Tr p ttc Phe Leu Asp Asp Asp Ser at g let 580 ggt G y gct Al a acc Thr gga G y gaa G u 660 gca Al a ggc G y ct g Leu at c l Ie t ca Ser 740 gca Al a ct g Leu ct g Leu gcg Al a cct Pr a 820 565 cct Pr o cag G n gcg Al a tct Ser aat Asn 645 cca Pr o acg Thr aac Asn act Thr gcc Al a 725 ctt Leu t ac Tyr cgc Ar g caa G n aac Asn 805 t ca Ser 1843 1891 1939 1987 2035 2083 2131 2179 2227 2275 2323 2371 2419 2467 2515 2563 2611 tgg aac gtt tcc gaa gag ggt ctc aag cgt acc gac gag ttt ctt gat Page 136 Trp Asn Val Ser G u G u Gy Leu L 825 8 ggc gaa cat gtc gca ggc atc aag c Gy u His Val Ala Gy Ile Lys A 840 845 cgc act gcc cgt gct ctg cgc aac c Arg Thr Ala Arg Ala Leu Arg Asn A 855 860 taagtaaaag attctcaatc cca <210> 104 <211> 867 <212> PRT <213> Cor ynebact eri um gl ut ai cum 1 i st cor r ect ed. t xt .ys Arg Thr Asp G u Phe Leu Asp 30 835 ga att gtt tcc gaa tcc ctc gac ,rg lie Val Ser u Ser Leu Asp 850 gt gcg gca gat gct gcg rg Ala Ala Asp Ala Ala 865 2659 2701 2724 <400> 104 Leu Thr Ser 1 Leu Leu Ser Asp G u Phe Al a y Asp Arg Leu Asp Asn G y Tyr G y Al a Hi s Gy Gu y 115 Leu Tyr Thr 130 Phe Asp G n 145 Pro Lys G y G n Hi s Thr Ser Thr Tyr 195 Asp Val Tr p 210 G n Pro Thr 225 Leu Ala Lys Thr Asn Leu Thr 5 Val G u Asn Tyr Phe Ser Ser Ser Thr Phe I e Asp Asn Val Ser Ile 70 Asp G u Thr Phe Thr Leu Arg Val 100 Leu His Arg IVet G n Phe G u Thr 135 Pro Asp Leu Lys 150 Trp Lys Ile Ile 165 Asp Tyr Asp Thr 180 Leu Ile Al a Val Lys G y Thr Leu 215 G u Leu Thr Val 230 Asp Leu Asp Ala 245 Ar g Asp Thr 40 Leu Lys Sy Thr Val 120 Al a Al a Ser Hi s Cys 200 Thr Pr o Val G n I e Val Ar g Asp SIle Al a 105 Asp Asp Thr Asn SIle 185 Al a Hi s Leu Ar g G u 10 Al a Val Al a G u Al a 90 Ser Pr o Al a Tyr Ser 170 Ser Sy Hi s Al a Al a Leu Ser Al a Al a 75 Leu SIle Al a Lys Asp 155 G u Ar g Ar g Al a Leu 235 Asp Leu Thr Val Thr Sy Tyr Asn 125 Met Asn G n Asp Hi s 205 Thr Cys Ser Arg Asn y Arg Lys G u Val G y Lys Thr Pro Arg Thr Val Tyr Al a Cys Lys Thr 160 Ser Thr 175 Pro Leu Val Cys Al a Asp Ser Ser 240 Lys G n 255 Leu Phe Thr G u Thr 250 Page 137 Sy Phe Asp Trp Tyr 260 seql i st cor r ect ed. t xt His Arg Asn Phe y Val Ala Tyr Pro Phe G y 270 Lys Asn Al a 305 Leu Asp G n Val Pr o 385 Asp Al a Al a Leu Leu 465 Asn Sy Leu Ser Asp 545 Leu Ser Thr Ar g Asp 275 Sy Ar g Hi s Tr p G u 355 Lys Phe SIle Val Hi s 435 G n Thr Lys Sy Asp 515 Al a Val Asp Pr o Al a 595 Al a SIle Val Ar g Tr p 325 Asn Thr Tr p Asp Tyr 405 Ar g Tr p Ser Sy Thr 485 Leu Ser Thr Val Ser 565 Pr o G n Al a Phe Thr Tyr 310 Phe G u G u Al a Gy 390 Al a G u Gy Gy I e 470 Ser Ar g Leu Ser Asn 550 Ar g Ar g bet Gu Val SIle 295 G u Gy Ser Tyr Tyr 375 Tyr Lys G u Asn Ar g 455 Asn Phe Thr Asn Val 535 Asp Asn Thr Lys Thr 615 Pr o 280 Ar g Ar g Val Phe Asn 360 G n Asp Sy Phe Al a 440 Asp Thr Ser Hi s Ar g 520 G u Asp Phe Leu Al a 600 G u G u Phe Asn Asp G u Tyr Ar g Al a G u 315 Leu Val Thr 330 Al a Thr Trp 345 Thr Al a Trp Sn Asp n i e G u Thr 395 Al a Ser Val 410 Leu Al a G y 425 Ser Phe Asp Leu Ser Asp Leu G y Al a 475 Val Thr G n 490 Ar g I e Al a 505 Tyr Al a Arg G u Ile Val Asp Leu Thr 555 Val Ile Asp 570 Val Trp Ser 585 Arg Asp Phe I e Al a Val Al a Val 300 Thr Met Ser Val Leu 380 Val Leu Val Asp Tr p 460 Lys Thr Val Val Sy 540 Tyr Asn Al a SIle Sy 285 Phe SIle G n Al a Thr 365 Pr o Asp Lys Ar g Leu 445 Al a Phe Sy Sy G u 525 Leu Al a SIle Al a Al a 605 Al a Al a Leu Tr p Al a 350 Phe Ser G n G n Ar g 430 Leu Asn Thr Al a Leu 510 Leu G u Leu Asp Tr p 590 Leu bet Ser Hi s Tr p 335 l le Al a Thr Asn Leu 415 Hi s Sy G n Thr Al a 495 Tyr Asp G n Leu Lys 575 G u Val G u Lys G u 320 Asp Ser Asn Hi s Phe 400 G n Phe Al a Tr p Asp 480 Pr o Lys Cys Al a Asp 560 Phe Met Al a Leu G u Arg lie Leu 620 Page 138 seql i st cor r ect ed. t xt Ala n Ala Thr Ser Al a Leu Lys Ser 625 630 Tyr Ala Asp Pro Ala Trp Ala G u Ar g Leu SIle Tr p 705 G u Phe Lys Asn Ser 785 Asp Gy Asp Ser Asp 865 Thr Al a Lys 675 Al a Al a Al a Al a Al a 755 G u Leu Tr p Phe Phe 835 Ser Al a Asn 645 Pr o Thr Asn Thr Al a 725 Leu Tyr Ar g G n Asn 805 Ser Asp Asp Asp Leu Val Al a 710 Al a Ar g Lys Hi s Al a 790 Phe Tr p Gy Leu Ser Asn G u 695 Leu G u Al a Hi s Lys 775 Tyr Ser Asn G u Al a Thr 665 Al a Leu Al a Ser Al a 745 Thr G u G u G u Ser 825 Val Asp 650 G n Al a Thr Ar g Ar g 730 Al a Al a Gy G n Met 810 G u Al a 635 Al a Leu Al a Val Gy 715 Asp Val Val Leu Tyr 795 Al a G u Gy Leu Phe Tyr 685 Pr o SIle Ser Thr Ser 765 Phe G u G n Leu Lys 845 Gy 655 G n Ar g Leu Al a Al a 735 G u Leu Sy Leu Val 815 Ar g SIe Leu Asp Arg Thr Ala Arg Ala Leu 855 Asn Arg Ala Ala <210> 105 <211> 1578 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (1)..(1578) <223> FRXA01014 <400> 105 gat gat ctg tgg ctc aac gag tcc ttc gcc act tgg tcc gcg gca att Asp Asp Leu Trp Leu Asn u Ser Phe Ala Thr Trp Ser Ala Ala lie 1 5 10 tct cag gct gag gaa act gaa tac aac act gca tgg gtg act ttc gcc Ser G n Ala G u G u Thr G u Tyr Asn Thr Ala Trp Val Thr Phe Ala 25 Page 139 seql i st cor r ect ed. t xt aat Asn cac Hi s ttc Phe cag G n ttt Phe gcc Al a tgg Tr p gac Asp 145 ccg Pr o aag Lys tgc Cys gct Al a gat Asp 225 ttc Phe at g Met gct Al a ct c Leu gag gt g Val ggc G y t ac Tyr aac Asn gag G u 115 aag Lys ggc G y gcc Al a gt c Val ggc G y 195 ttc Phe gat Asp gac Asp cgc Ar g ggc G y 275 cag G n tcg Ser t ct Ser acc Thr ggc G y gca Al a t cc Ser acc Thr t ac Tyr gag G u 165 gga G y tcg Ser ct g Leu gat Asp at g Met 245 ggt Sy gct Al a acc Thr tgg Tr p gac Asp tac Tyr 70 cgt Ar g tgg Tr p t cc Ser ggc G y acc Thr 150 ct g Leu t cc Ser aca Thr gt c Val t ca Ser 230 cct Pr o cag G n gcg Al a tct Ser gcg Al a gga G y gca Al a gag G u ggc G y ggc G y at c SIle 135 t cc Ser cgg Ar g ct c Leu agc Ser aac Asn 215 cgc Ar g cgc Ar g at g Met gaa G u gcg Al a 295 t ac Tyr 40 t ac Tyr aag Lys gaa G u aac Asn cgc Ar g 120 aac Asn ttc Phe act Thr aac Asn gtt Val 200 gat Asp aat Asn acg Thr aag Lys act Thr 280 ct g Leu cag cag gat cag Sn n Asp n gac at t gag act Asp Ile G u Thr ggc gcc tcg gt g G y Ala Ser Val 75 ttc ctg gca ggc Phe Leu Ala G y 90 gcc agc ttt gat Ala Ser Phe Asp 105 gac ctc tcc gac Asp Leu Ser Asp acc ctc ggc gca Thr Leu y Ala 140 tcc gtc acc cag Ser Val Thr G n 155 cac cgc atc gcg His Arg Ile Ala 170 cgc tac gca cga Arg Tyr Ala Arg 185 gaa gag atc gtt G u G u Ile Val gat gat ctg acg Asp Asp Leu Thr 220 ttt gtc atc gac Phe Val lie Asp 235 ctg gtg tgg tcc Leu Val Trp Ser 250 gct cgt gat ttc Ala Arg Asp Phe 265 gaa att gct gtg G u I I e Al a Val ct g cct Leu Pro gtc gac Val Asp ctc aag Leu Lys gta cgc Val Arg gat ctg Asp Leu 110 tgg gca Trp Al a 125 aag ttc Lys Phe acc ggc Thr G y gtg ggt Val G y gta gaa Val G u 190 gga ctt G y Leu 205 tat gcg Tyr Al a aat at t Asn II e gct gcg Al a Al a atc gcg I e Al a 270 ctg gag Leu G u 285 t cc Ser cag G n cag G n agg Ar g ct c Leu aac Asn acc Thr gcc Al a ctt Leu 175 ctt Leu gag G u ct g Leu gat Asp tgg Tr p 255 ct g Leu cgc Ar g acc Thr aac Asn ctg Leu cac Hi s ggc G y cag G n acc Thr gcg Al a 160 t at Tyr gac Asp cag G n ctg Leu aag Lys 240 gag G u gtt Val att SIe aag Lys agc tac gcc gac cca gcg tgg Ser Tyr Ala Asp Pro Ala Trp 300 Page 140 seql i st cor r ect ed. t xt gca Al a 305 gct Al a gct Al a gac Asp cgt Ar g gt c Val 385 t cc Ser gt g Val t cc Ser tct Ser gat Asp 465 ct c Leu acc Thr gtt Val gca Al a t cc Ser gca Al a ctt Leu 355 tgg Tr p gat Asp ct c Leu gct Al a ct g Leu 435 gaa Gu gt g Val ct g Leu gag G u gaa Gu 515 act gga aat gac Thr y Asn Asp 310 gca gaa cca gac Ala G u Pro Asp 325 aaa gca acg ctc Lys Ala Thr Leu 340 gcc ggc aac gt c Al a G y Asn Val gca ctg act gcg Ala Leu Thr Al a 375 gca atc gcc gct Ala IIe Ala Al a 390 gca tca ctt cga Ala Ser Leu Arg 405 gct gca tac aag Al a Al a Tyr Lys 420 gag ctg cgc cac G u Leu Arg His ctg ctg caa gcc Leu Leu G n Al a 455 tgg gcg aac ttc Trp Ala Asn Phe 470 ttc cct tca tgg Phe Pro Ser Trp 485 ttt ctt gat ggc Phe Leu Asp G y 500 tcc ctc gac cgc Ser Leu Asp Arg ctg ctg gcc gat gct Leu Leu Ala Asp Ala 315 tcc gac act cag ttg Ser Asp Thr G n Leu 330 aat gat gct gct gcc Asn Asp Al a Al a Al a 345 gaa ggc ctg acc gtg G u G y Leu Thr Val 360 ctt atc gcc cgt ggt Leu lie Ala Arg y 380 gaa ctt tcc cgc gac G u Leu Ser Arg Asp 395 gcc ggt gcc gct gtg Al a y Al a Al a Val 410 cat gtc ccg gca gtt His Val Pro Ala Val 425 aag att gaa ggc ctc Lys Ile G u G y Leu 440 tac aac gag cag tac Tyr Asn G u G n Tyr 460 tcc ggc gaa atg gca Ser G y G u Vet Al a 475 aac gtt tcc gaa gag Asn Val Ser G u G u 490 gaa cat gtc gca ggc Su His Val Ala y 505 act gcc cgt gct ct g Thr Ala Arg Ala Leu 520 ttc ctt gag Phe Leu G u gcg ttc att Al a Phe Il e 335 gat tac ttc Asp Tyr Phe 350 gat cct gac Asp Pro Asp 365 gac at c gag Asp I e G u aac t cc agt Asn Ser Ser aac act gaa Asn Thr G u 415 gat agt ggc Asp Ser G y 430 aca ttc act Thr Phe Thr 445 ttc gaa at c Phe G u Ile cag cag at c Gn n lie ggt ct c aag G y Leu Lys 495 atc aag cga Ile Lys Ar g 510 cgc aac Arg Asn 525 ggt G y 320 cag G n cgc Ar g ct g Leu gct Al a gcc Al a 400 gaa G u ct a Leu ggc G y ctt Leu gt c Val 480 cgt Ar g at t SIe 960 1008 1056 1104 1152 1200 1248 1296 1344 1392 1440 1488 1536 1578 <210> 106 <211> 526 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 106 Asp Asp Leu Trp Leu Asn u Ser Phe Ala Thr Trp Ser Ala Ala lie 1 5 10 Ser G n Ala G u G u Thr G u Tyr Asn Thr Ala Trp Val Thr Phe Ala Page 141 seqi i st car r ect ed. t xt O31n O31n As p O31n Pr o Ser Thr As n Hi s Ph e O31n Ph e A a Tr p As p 145 Pr o Ly s cys A a As p 225 Ph e vb t A a Le u A a 305 A a A a As p Ar g O31u ValI O31y Tyr As n O31u 115 Ly s O31y A a ValI O31y 1 95 Ph e As p As p Ar g O31y 275 O31n A a Ser A a Le u 355 Tr p Ser Ser Thr O31y A a Ser Thr Tyr O31u 1 65 O31y Ser Le u As p vb t 245 O31y A a Thr O31y O31u 325 A a O31y Le u Tr p As p Tyr 70 Ar g Tr p Ser O31y Thr 150 Le u Ser Thr ValI Ser 230 Pr o O31n A a Ser As n 310 Pr o Thr As n Thr A a (31y A a O31u O31y O31y I Ie 135 Ser Ar g Le u Ser As n 215 Ar g Ar g vb t O31u A a 295 As p As p Le u ValI A a 375 As p O31y Ph e A a 1 05 As p Thr Ser Hi s Ar g 1 85 O31u As p Ph e Le u A a 265 O31u Ly s Le u As p As p 345 O31y I Ie I Ie A a Le u 90 Ser Le u Le u ValI Ar g 1 70 Tyr O31u As p ValI ValI 250 Ar g I Ie Ser A a Thr 330 A a Le u (31u Ser A a Ph e Ser O31y Thr 1 li1e A a I Ie Le u I Ie 235 Tr p As p A a Tyr As p 315 O31n A a Thr Thr ValI O31y As p As p A a 140 O31n A a Ar g ValI Thr 220 As p Ser Ph e ValI A a 300 A a Le u A a ValI Le u ValI Le u ValI As p Tr p 125 Ly s Thr ValI ValI O31y 205 Tyr As n A a I Ie Le u 285 As p Ph e A a As p As p 365 As p Ly s Ar g Le u 110 A a Ph e O31y O31y O31u 1 Le u A a I Ie A a A a 270 O31u Pr o Le u Ph e Tyr 350 Pr o (31n O31n Ar g Le u As n Thr A a Le u 1 Le u O31u Le u As p Tr p 255 Le u Ar g A a Ou le 335 Ph e As p Ala Ar g O31y 380 Asp I I e 03 u A a Page 142 Val 385 Ser Val Ser Ser Asp 465 Leu Thr Val Asp Leu Al a Leu 435 G u Val Leu G u G u 515 SIle Ser 405 Al a Leu Leu Al a Pr o 485 Leu Leu Al a 390 Leu Tyr Ar g G n Asn 470 Ser Asp Asp Al a Ar g Lys Hi s Al a 455 Phe Tr p Gy Ar g seql i st cor r ect ed. t xt G u Leu Ser Arg Asp Asn 395 Ala Gy Ala Ala Val Asn 410 His Val Pro Ala Val Asp 425 Lys lie G u G y Leu Thr 440 445 Tyr Asn G u G n Tyr Phe 460 Ser G y G u bet Ala G n 475 Asn Val Ser u u y 490 G u His Val Ala G y IIe 505 Thr Ala Arg Ala Leu Arg 520 525 Ser G u 415 Gy Thr SIe SIe Lys 495 Ar g <210> 107 <211> 964 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(964) <223> FRXA01018 <400> 107 tcttaaagtt ttctagcaat ccacactagg cgcgaactat tctaagggta gcgccccctc aaatttcaag gagcattaaa ct c Leu aac Asn t cc Ser at t SIle t cc Ser acg Thr cga Ar g gac Asp acc Thr ct c Leu aaa Lys ggc G y gaa G u gca Al a gt c Val gca Al a gag G u gcc Al a gct Al a ctt Leu agc Ser gca Al a gct Al a 75 ct g Leu tcg Ser gat Asp ttc Phe agc Ser ct a Leu aag Lys cgt Ar g aac Asn gt c Val gag G u ctt Leu ctt Leu tcg agg Ser Arg 15 aac ggt Asn y agg aag Arg Lys gag gtt G u Val ggc aag G y Lys 80 act ccc Thr Pro 95 Page 143 cgt ggt gt ca t t gcgcacct ttg acg tcc act aat Leu Thr Ser Thr Asn 1 tta ctg agt gta gaa Leu Leu Ser Val G u gat gag ttt ttt agt Asp G u Phe Phe Ser gct ggc gat acc ttt Ala G y Asp Thr Phe cgc ctg gac aat gtg Arg Leu Asp Asn Val aac ggc tac gac gag Asn G y Tyr Asp G u ggc gcg cac acc ttg G y Ala His Thr Leu 100 IN seql i st cor r ect ed. t xt cgg gta acg gcg tct atc ccc tat tcc cgc acc ggt gaa ggc ctg cac 451 C' Arg Val Thr Ala Ser I e Pro Tyr Ser Arg Thr G y G u G y Leu His 105 110 115 cgc atg gtg gat cca gca gac aat gag gtg tat ttg tac acc cag ttt 499 L Arg Vet Val Asp Pro Ala Asp Asn G u Val Tyr Leu Tyr Thr G n Phe 120 125 130 gag acc gcc gat gcc aag cgt atg ttc gcg tgt ttc gat cag cca gac 547 G u Thr Ala Asp Ala Lys Arg IVet Phe Ala Cys Phe Asp G n Pro Asp 135 140 145 (N 0 ctc aag gct acc tat gat ctg aac atc aaa act cct aag ggt tgg aag 595 00 Leu Lys Ala Thr Tyr Asp Leu Asn Ile Lys Thr Pro Lys Gy Trp Lys S150 155 160 165 C1 atc att tcc aac tct gag cag cag gtt tcc act cag cac act gat tac 643 \O Ile Ile Ser Asn Ser G u Gn n Val Ser Thr n His Thr Asp Tyr 170 175 180 CK1 gat acc cac att tcc cga gtg gac tat ccc ctc tcc acc tac ctg att 691 Asp Thr His lie Ser Arg Val Asp Tyr Pro Leu Ser Thr Tyr Leu lie 185 190 195 gcg gtg tgc gcg ggt cgt tac cac gag gtg tgc gat gtc tgg aag ggt 739 Ala Val Cys Ala G y Arg Tyr His G u Val Cys Asp Val Trp Lys G y 200 205 210 acg ctc acc cac cat gca gaa aca cct gcc gat cag cca act gag ctg 787 Thr Leu Thr His His Al a u Thr Pro Ala Asp G n Pro Thr G u Leu 215 220 225 act gtt ccg ctt gct ctc tac tgc cgc agt tct ttg gct aaa gat ctt 835 Thr Val Pro Leu Ala Leu Tyr Cys Arg Ser Ser Leu Ala Lys Asp Leu 230 235 240 245 gat gcg gtg cgt ctg ttt acc gaa acg aag cag ggc ttt gat tgg tac 883 Asp Ala Val Arg Leu Phe Thr G u Thr Lys G n y Phe Asp Trp Tyr 250 255 260 cac cgc aac ttc ggt gtg gcg tac cca ttc ggc aag tac gat cag atc 931 His Arg Asn Phe G y Val Ala Tyr Pro Phe G y Lys Tyr Asp G n I e 265 270 275 ttc gtc cct gaa ttt aat gct ggc gcg atg gag 964 Phe Val Pro O u Phe Asn Al a G y Al a Iet G u 280 285 <210> 108 <211> 288 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 108 Leu Thr Ser Thr Asn Leu Thr Arg n G u Ala Ser Asp Arg Ser Arg 1 5 10 Leu Leu Ser Val G u Asn Tyr Asp Ile Ala Leu Asp Leu Asn Asn G y 25 Asp G u Phe Phe Ser Ser Ser Thr Val Val Ser Phe Thr Val Arg Lys 40 Ala y Asp Thr Phe I le Asp Leu Arg Ala Ala Ser Val u u Val 55 Page 144 Ar g Asn Gy Gy Leu Phe 145 Pr o G n Ser Asp G n 225 Leu Sy Lys Leu Asp Asn Val Ser Ile Ly 70 seql i st cor r ect ed. t xt s Asp G u Ala Leu Thr Leu G y Lys Tyr Hi s Gy 115 Thr G n Gy Thr Tyr 195 Tr p Thr Lys Asp Asp 275 G u Leu Hi s Phe Asp Lys 165 Tyr SIle Gy Leu Leu 245 Tyr SIle Thr Ar g Ar g G u Leu 150 I le Asp Al a Thr Thr 230 Asp Hi s Phe Phe Val let Thr 135 Lys SIle Thr Val Leu 215 Val Al a Ar g Val Gy Thr Val 120 Al a Al a Ser Hi s Cys 200 Thr Pr o Val Asn Pr o 280 SIle Al a 105 Asp Asp Thr Asn SIle 185 Al a Hi s Leu Ar g Phe 265 G u Al a 90 Ser Pr o Al a Tyr Ser 170 Ser Gy Hi s Al a Leu 250 Gy Phe Leu SIle Al a Lys Asp 155 G u Ar g Ar g Al a Leu 235 Phe Val Asn Lys Pr o Asp Ar g 140 Leu G n Val Tyr G u 220 Tyr Thr Al a Al a Gy Tyr Asn 125 Met Asn G n Asp Hi s 205 Thr Cys G u Tyr Sy 285 Thr Ar g Val Al a Lys Ser 175 Pr o Val Al a Ser Lys 255 Phe let <210> 109 <211> 1383 <212> DNA <213> Corynebacteri um gl utami cum <220> <221> CDS <222> (101)..(1360) <223> RXA01147 <400> 109 ataatgatca cctactttaa cggcttcagg tgacattgtg gattcgcatt gtggattcgg gggcccgcgc tgtttccaag aatttggcta cccttgttct atg cat gta act gac lMt His Val Thr Asp 1 gat ttc tta agt ttt att gcc cta agc cca agt tcc tat cac gcg gcc Asp Phe Leu Ser Phe lie Ala Leu Ser Pro Ser Ser Tyr His Ala Ala 15 gcg gcg gtg gag cgc agg ttg ctc cat gag ggg ttc att cgt cag gaa Page 145 seql i st cor r ect ed. t xt Ala Ala Val G u Arg Arg Leu Leu His G u Gy Phe II 30 e Arg n G u gat Asp ggg Gy t cc Ser tta Leu gt c Val ct g Leu gt c Val gac Asp 150 cag G n at t SIle ct g Leu gat Asp t cc Ser 230 gat Asp t cc Ser act Thr gat Asp gca Al a ttc Phe ccc Pr o gtt Val tta Leu 120 acc Thr act Thr gt g Val ggt Sy acg Thr 200 ttg Leu act Thr ttg Leu tcg Ser cgt Ar g 280 tgg Tr p gt g Val at c SIle ggg G y ggc G y ggc G y ccg Pr o aat Asn gct Al a 170 gcg Al a gct Al a tct Ser ttg Leu ctt Leu 250 gcc Al a ttg Leu gat Asp gcg Al a at t SIle 75 gat Asp gga G y cgc Ar g at t SIle t cc Ser 155 gtt Val gt a Val acc Thr ggt G y at t SIle 235 gct Al a ggg gty ggt G y gcc Al a tgg Tr p ggg G y ctt Leu ccg Pr o at t SIle ct g Leu 140 caa G n ggt G y gt g Val caa G n cgc Ar g 220 gcg Al a gca Al a ggc G y gca Al a cgc Ar g 45 tgg Tr p t ca Ser t cc Ser at c SIle gt g Val 125 cgc Ar g ct c Leu gag G u gat Asp gat Asp 205 ct g Leu gct Al a ttc Phe ccc Pr o gat Asp 285 cct Pr o gt g Val cat Hi s t ca Ser ct g Leu 110 ctt Leu at c SIle acc Thr ccc Pr o cct Pr o 190 gct Al a gat Asp tcg Ser gat Asp ct g Leu 270 gaa G u ggt G y cct Pr o act Thr cac Hi s 95 cca Pr o gcc Al a ccg Pr o ctt Leu gac Asp 175 gca Al a gaa G u aac Asn caa G n cat Hi s 255 ttg Leu gat Asp ggg G y gag G u gat Asp 80 ggt G y agc Ser gac Asp cac Hi s aat Asn 160 gt a Val gat Asp gt a Val ct g Leu tct Ser 240 gaa ggu gag G u gag G u cat Hi s gat Asp t ca Ser tgg Tr p tgg Tr p ggg G y gt g Val 145 cca Pr o t ca Ser at t SIle ttt Phe agc Ser 225 gac Asp gaa G u gat Asp cga Ar g gt g Val gct Al a ccg Pr o cag G n ct g Leu t cc Ser 130 gct Al a cag G n at t SIle gt c Val ggc G y 210 agc Ser gat Asp gt g Val gt g Val cgc Ar g 290 gt g Val cca Pr o ttc Phe gcc Al a 100 cgc Ar g aag Lys cat Hi s cac Hi s gat Asp 180 cat Hi s cat Hi s cat Hi s ggt G y agt Ser 260 aac Asn at g let cgt Ar g gat Asp aag Lys ggc G y gag G u ctt Leu ttg Leu ctg Leu 165 gtc Val gat Asp ggg G y cca Pr o tcg Ser 245 aat Asn cgt Ar g ttt Phe aac cgt tcc acc atg gtc tca gct gac gcg gca cac tcc att cac ccc Page 146 1027 IN seql i st cor r ect ed. t xt O Asn Arg Ser Thr Mbt Val Ser Ala Asp Ala Ala His Ser Ile His Pro 295 300 305 aac ttc ccc gag aag cat gat caa gct aat tac ccc atc att ggt aaa 1075 SAsn Phe Pro u Lys His Asp n Ala Asn Tyr Pro lie lie y Lys d) 310 315 320 325 Sggt cct gta ttg aag gtc aac gcc aac cag cgc tac acc tcc gat gca 1123 C 1 y Pro Val Leu Lys Val Asn Ala Asn G n Arg Tyr Thr Ser Asp Ala 330 335 340 gtc act tca ggc atg tgg atc agg gca tgt cag att gcc ggt gtg cca 1171 C- Val Thr Ser G y IVet Trp Ile Arg Ala Cys G n Ile Ala G y Val Pro 345 350 355 00 0 cac cag gtg ttt gcc ggc aac aac gat gtg ccg tgt ggt tcc acc atc 1219 0 His G n Val Phe Ala G y Asn Asn Asp Val Pro Cys y Ser Thr Ile C1 360 365 370 IO 0 ggc ccg atc agt gcg act cgc ctg ggt atc gat tct gtc gat gtc ggt 1267 G y Pro Ile Ser Ala Thr Arg Leu G y Ile Asp Ser Val Asp Val y Cr 375 380 385 att cca ttg ctg tcc atg cac tcc gca cgc gaa atg gcc gga gtg aag 1315 IIe Pro Leu Leu Ser IVet Hi s Ser Al a Arg G u Iet Al a G y Val Lys 390 395 400 405 gat ctg atg tgg ttt gaa caa gcc ctg gaa gcc tat ctg gta aat 1360 Asp Leu IVt Trp Phe G u G n Ala Leu G u Ala Tyr Leu Val Asn 410 415 420 taacgccgag ttcaatcaag aca 1383 <210> 110 <211> 420 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 110 IVet His Val Thr Asp Asp Phe Leu Ser Phe I e Ala Leu Ser Pro Ser 1 5 10 Ser Tyr His Ala Ala Al a Ala Val G u Arg Arg Leu Leu His G u y 25 Phe lie Arg n G u Asp Thr Asp G u Trp Asp Ala Arg Pro y y 40 His Val Thr Val Arg G y G y Ala Val Val Ala Trp Trp Val Pro O u 55 Asp Ala Ser Pro Asp Ser G y Phe Arg lie lie G y Ser His Thr Asp 70 75 Ser Pro G y Phe Lys Leu Lys Pro Arg G y Asp Leu Ser Ser His G y 90 Trp n n Ala y Val u Val Tyr y y Pro Ile Leu Pro Ser 100 105 110 Trp Leu Asp Arg G u Leu Ala Leu Ala y Arg lie Val Leu Ala Asp 115 120 125 G y Ser Val Lys Leu Val Asn Thr G y Pro lie Leu Arg lie Pro His 130 135 140 Val Ala lle His Leu Asp Arg Thr Val Asn Ser G n Leu Thr Leu Asn Page 147 seql i st cor r ect ed. t xt Pr o Ser SIle Phe Ser 225 Asp G u Asp Ar g Hi s 305 Pr o Tyr SIle Cys Ser 385 bet Tyr Arg Hi s Leu Asp 180 Ser His 195 Al a Hi s Val Hi s Thr G y G y Ser 260 Leu Asn 275 Arg IVet Ile Hi s I e G y Ser Asp 340 G y Val 355 Ser Thr Asp Val G y Val Val Asn 420 Leu 165 Val Asp Sy Pr o Ser 245 Asn Ar g Phe Pr o Lys 325 Al a Pr o SIle Sy Lys 405 150 Sn Pro Val I e Al a G y Leu II e Thr 200 Asp Phe Leu 215 Ser Met Thr 230 Asp Ile Leu Ser Thr Ser Thr Al a Ar g 280 Asn Arg Ser 295 Asn Phe Pro 310 Sy Pro Val Val Thr Ser His G n Val 360 Sy Pro lle 375 Ile Pro Leu 390 Asp Leu IVet 155 Phe Al a Val 170 Al a Al a Val 185 Val Al a Thr Al a Ser G y Al a Leu II e 235 Val Leu Al a 250 G y Al a G y 265 Al a Leu G y Thr Met Val G u Lys Hi s 315 Leu Lys Val 330 G y bet Tr p 345 Phe Al a G y Ser Al a Thr Leu Ser IVet 395 Trp Phe G u 410 G y G u Pr o Val G n Ar g 220 Al a Al a Sy Al a Ser 300 Asp Asn SIle Asn Ar g 380 Hi s G n Asp Asp 205 Leu Al a Phe Pr o Asp 285 Al a G n Al a Ar g Asn 365 Leu Ser Al a Pr o 190 Al a Asp Ser Asp Leu 270 G u Asp Al a Asn Al a 350 Asp Sy Al a Leu 160 Asp Val 175 Al a Asp G u Val Asn Leu G n Ser 240 Hi s G u 255 Leu G u Asp u Al a Al a Asn Tyr 320 G n Arg 335 Cys n Val Pro I e Asp Arg G u 400 G u Al a 415 <210> 111 <211> 1260 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1237) <223> RXA01161 <400> 111 tttttcgtga tcaacaatcc gctggcatag cgtccagcag atttgattct gacagtgtgg tttgatcgca cacctgccta ggctactagg gt t ggagact atg agt gat cct tca 115 Page 148 seql i st cor r ect ed. t xt bet Ser Asp Pro Ser aca Thr caa G n ggc Gy cgt Ar g ctt Leu ct a Leu ttg Leu ggt Sy ggc G y ttt Phe 150 gca Al a gga Gy gaa G u ggc Gy gac Asp 230 tct Ser aat Asn ggc Gy gaa G u acc Thr gct Al a at c SIle gt a Val t cc Ser 120 acc Thr tct Ser at t l Ie acc Thr cac Hi s 200 aac Asn gt g Val tgc Cys ccc Pr o cgc Ar g gcg Al a ttg Leu gac Asp gt g Val gct Al a acg Thr cgc Ar g gac Asp cgt Ar g 170 gca Al a gag G u cac Hi s gtt Val cgc Ar g 250 aca Thr aag Lys t at Tyr gt g Val cgc Ar g 75 gcc Al a ct c Leu gca Al a at g Met gaa G u 155 gt c Val gag G u gt g Val cac Hi s gat Asp 235 acc Thr tcg Ser gct Al a ct a Leu at c SIle gga Gy gga Gy ggt G y gat Asp gag G u 140 gca Al a cac Hi s gtt Val gac Asp ggt Gy 220 at a SIle t ac Tyr gt a Val ggc Gy acc Thr 45 ccc Pr o gac Asp tgg Tr p gtt Val cac Hi s 125 ttg Leu gag G u gcc Al a gca Al a ttc Phe 205 ttc Phe gga Gy at t SIle t at Tyr ttg Leu ggc Gy agc Ser tta Leu gtt Val t ca Ser 110 ct g Leu gca Al a at c SIle aaa Lys gca Al a 190 gt g Val tct Ser ggc Gy gt g Val gca Al a 15 aac Asn agc Ser gaa G u gca Al a gat Asp 95 gag G u at t SIle gtt Val gag G u gt c Val 175 cag G n at t SIle gac Asp acc Thr ggc G5y 255 cag G n ggt Gy tgg Tr p gga Gy ct g Leu 80 ggc Gy ttc Phe cct Pr o caa G n cag G n 160 ccg Pr o ct c Leu gt g Val cga Ar g ttc Phe 240 gga G y ctt Leu at c SIle t cc Ser gca Al a gct Al a aat Asn gca Al a cag G n 130 ct g Leu cgc Ar g ctt Leu gat Asp t cc Ser 210 ct c Leu cct Pr o cct Pr o gcg Al a at c I e acc Thr acc Thr at t l Ie gcc Al a ttg Leu 115 aac Asn aaa Lys ggc G y ctt Leu ct c Leu 195 gct Al a cgc Ar g ggt G y gac Asp gat gca Asp Al a ggt aca G y Thr cat gag Hi s G u att gtt Ile Val cca gga Pro G y cat gag Hi s G u 100 ggt att G y lie ctg gtg Leu Val gaa ttg G u Leu gca ggt Al a G y 165 caa gac Sn Asp 180 atc ttg I e Leu gaa aac Su Asn aat ggc Asn y tac cac Tyr His 245 gat gcg Asp Al a 260 gat cca gag ttc gct aag ttc tac caa gtg ctc tac gaa gca cag ctc Page 149 seql i st cor r ect ed. t xt Asp Pro G u Phe Ala Lys Phe Tyr G n Val Leu Tyr G u 265 270 gca gcc gtt gcg cat gtt cgc cct ggc gtt act gca gaa Ala Ala Val Ala His Val Arg Pro G y Val Thr Ala G u 280 285 290 gct gtt gct cgc gat cac at t gct gcg gct gga t ac ggc Ala Val Ala Arg Asp His IIe Ala Ala Ala G y Tyr G y 295 300 305 att cac cgc aca gga cac ggc att ggt cta tcc acc cat lie His Arg Thr G y His G y lie G y Leu Ser Thr Hi s 310 315 320 ttc atc atg gcg ggt aac tca ctc gtg ttg gaa gcc gga Phe Ile IVet Al a G y Asn Ser Leu Val Leu G u Al a G y 330 335 tcc att gag cct ggc atc tac att gaa gga atc cac gga Ser lie G u Pro Gy ile Tyr lie G u Gy lie His y 345 350 gaa gac atc gtt gtg gtg aat gaa gac ggt tgt gaa acc G u Asp lie Val Val Val Asn G u Asp y Cys G u Thr 360 365 370 cag ccc aag gaa ctg cgt tgagcattct tctcctaggc gga G n Pro Lys G u Leu Arg 375 <210> 112 <211> 379 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 112 IVet Ser Asp Pro Ser Thr Asn Asn Phe Pro Thr Ser Val 1 5 10 Arg Leu Ala Asp Ala On Ou Gy Ala Arg Lys Ala Gy 25 Leu lie lie Gy Thr Gy Ala u Leu Ala Tyr Leu Thr 40 Ile Ser Thr His G u Arg Leu Thr Ala Leu Val Ile Pro 55 Thr Ala Thr lie Val Leu Pro Ala Val Asp Arg y Asp 70 75 Ser Ala lie Pro Gy Leu Asp Ile Asn Val Al a y Trp 90 Asp Asn Ala His G u Leu Ala Val Asp Ala Leu G y Val 100 105 G u Ala Leu G y IIe G y Ser Ser IIe Thr Ala Asp Hi s 115 120 125 Ile G n Asn Leu Val G y Ser Thr Cys Arg Iet G u Leu 130 135 140 Val Leu Lys G u Leu Phe Val Ser Lys Asp G u Ala G u 145 150 155 Page 150 Al a 275 t ca Ser gaa G u gag G u at g Met gcg Al a 355 ct c Leu G n gt g Val t ac Tyr gag G u gcc Al a 340 cgc Ar g aac Asn 979 1027 1075 1123 1171 1219 1260 Tyr Al a G n Leu Asn G y G y Ser Trp Ser G u G y Leu Ala Leu Val Asp G y Ser G u Phe 110 Leu Ile Pro Ala Val n lie G u n Leu Arg G y Ala G y 165 seql i st cor r ect ed. t xt Ala Ala lle Asp Arg Val His Al a Lys Val Pro 175 G u Asn Gy Val 225 Gy Asn Tyr Al a Tyr 305 Thr Al a Hi s G u Leu Leu 195 Al a Ar g Gy Asp Al a 275 Ser G u G u bet Al a 355 Leu Asp Leu Asn Gy Hi s 245 Al a Leu Asp Phe Pr o 325 Phe l Ie Asn Gy G u Gy Asp 230 Ser Asp Al a Al a SIle 310 Phe Ser G u G n Ar g G u Al a 215 I le Asp Pr o Al a Val 295 Hi s SIle SIle Asp Pr o 375 Thr Hi s 200 Asn Val Cys G u Val 280 Al a Ar g Met G u SIle 360 Lys G u 185 Ser Pr o Val Thr Phe 265 Al a Ar g Thr Al a Pr o 345 Val G u G u Val Hi s Asp 235 Thr Lys Val Hi s Hi s 315 Asn SIle Val Ar g Al a Phe 205 Phe Gy SIle Tyr Pr o 285 Al a SIle Leu SIle G u 365 Al a 190 Val Ser Gy Val G n 270 Gy Al a Gy Val G u 350 Asp G n SIe Asp Thr Gy 255 Val Val Al a Leu Leu 335 Gy Gy <210> 113 <211> 980 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> RXN01181 <400> 113 tct gta ctg ctc gct cgc gac ttg gtg Ser Val Leu Leu Ala Arg Asp Leu Val 1 5 tac cca gag tcc tac tca gta att gca Tyr Pro u Ser Tyr Ser Val Ile Al a 25 ggc ttg cag acc acc atc ctg gat gag Sy Leu n Thr Thr Ile Leu Asp u 40 ttc ggc ggc atc ctc gca gtc ggt aac Phe y y lie Leu Ala Val y Asn aac acc cct tca tca cac ctg Asn Thr Pro Ser Ser His Leu 10 tcc aac gaa gcg tcc aag cac Ser Asn G u Ala Ser Lys His aag cag ctt gct gat caa ggt Lys G n Leu Al a Asp n G y ggc tcc tcc cgc aag cct cgt Sy Ser Ser Arg Lys Pro Arg Page 151 seql i st cor r ect ed. t xt ct g Leu ttg Leu cct Pr o t cc Ser aac Asn gct Al a 145 gaa G u at t l Ie acc Thr gt c Val gag G u 225 gag G u cgt Ar g ggt Gy aac Asn cca Pr o 305 ct g cgc at c Leu Arg Il e gtt ggc aag Val G y Lys ggc gca agc G y Al a Ser 100 gta ttg gcc Val Leu Al a 115 gtc ttc gcg Val Phe Al a 130 ttc cgc ccc Phe Arg Pro atc ttg aac Ile Leu Asn gct tac gct Al a Tyr Al a 180 ctg act ggt Leu Thr G y 195 at g ggt acc IVet G y Thr 210 gtt ggc gag Val y u cag gtt aag G n Val Lys ttc gca gga Phe Al a G y 260 gcc gac at c Al a Asp I le 275 act gct ggt Thr Al a G y 290 gtg cgc acc Val Arg Thr gat tgg Asp Trp 70 ggc at c G y lle atg gag Mvt u acc at t Thr Ile ttc cta Phe Leu ggc gat Sy Asp 150 acc gac Thr Asp 165 tct gaa Ser G u gct caa Al a G n gat gag Asp u caa gca G n Al a 230 t cc cct Ser Pro 245 atg tct bet Ser gag tgg Su Trp gaa ttc G u Phe ttc gtt Phe Val 310 aag Lys acc Thr aac Asn at c SIle cca Pr o 135 gt c Val gct Al a gat Asp tta Leu ttc Phe 215 tgg Tr p gt c Val gct Al a gct Al a ggt Gy 295 cag G n cca Pr o ttt Phe at g Met gct Al a 120 at g Met at c SIle gaa G u aag Lys gt c Val 200 cgc Ar g gca Al a gct Al a gcg Al a cac Hi s 280 t ac Tyr gtt Val cgc Ar g gac Asp at c SIle 105 gca Al a gct Al a act Thr ggc Gy cct Pr o 185 gct Al a gac Asp at g Met gac Asp ggt Gy 265 gt c Val acg Thr ct g Leu aag Lys acc Thr 90 t cc Ser gct Al a gag G u cat Hi s cgc Ar g 170 gac Asp tta Leu agc Ser cct Pr o ct g Leu 250 cgt Ar g gat Asp cca Pr o aag Lys gct Al a 75 ggc Gy gac Asp cgt Ar g aac Asn ttc Phe 155 ct c Leu t ac Tyr ggc Gy gtt Val ctt Leu 235 cgc Ar g t ac Tyr at c SIle aag Lys gat Asp 315 aag Lys at t SIle ggt Gy aac Asn 125 cca Pr o ggt Gy ct g Leu at t SIle cgg Ar g 205 aag Lys gaa G u gt c Val cag G n ggc Gy 285 gca Al a at c SIle at c I e t cc Ser tcg Ser ggt Gy acc Thr gat Asp 175 gcg Al a t ca Ser ggc Gy ct c Leu aat Asn 255 ttc Phe gca Al a gga Gy gct Al a aag Lys gca Al a at c SIe gac Asp t cc Ser 160 gcc Al a gca Al a ggt Gy cgc Ar g gat Asp 240 t cc Ser gtt Val t ac Tyr caa G n tcg gaa agc Ser G u Ser taaacgctag ttaaagatca gga Page 152 seql i st cor r ect ed. t xt <210> 114 <211> 319 <212> PRT C) <213> Cor ynebact eri um gl ut ari cum 4 <400> 114 Ser Val Leu Leu Ala Arg Asp Leu Val Asn Thr Pro Ser Ser His Leu 1 5 10 Tyr Pro u Ser Tyr Ser Val Ile Ala Ser Asn G u Ala Ser Lys His 25 (N G y Leu n Thr Thr Ile Leu Asp u Lys n Leu Ala Asp Gn y 00 35 40 0 0 Phe Gy Gy lie Leu Al a Val y Asn y Ser Ser Arg Lys Pro Arg C(1 50 55 0 Leu Leu Arg lle Asp Trp Lys Pro Arg Lys Ala Lys Lys Ser lie Ala S 65 70 75 (N Leu Val y Lys Gy Ile Thr Phe Asp Thr Gy Gy lie Ser Ile Lys 90 Pro G y Ala Ser IVet G u Asn IVet lie Ser Asp Iet Gy G y Ser Ala 100 105 110 Ser Val Leu Ala Thr Ile lie Ala Ala Ala Arg Leu Asn Leu Ser Ile 115 120 125 Asn Val Phe Ala Phe Leu Pro Iet Ala G u Asn Iet Pro Ser y Asp 130 135 140 Ala Phe Arg Pro G y Asp Val lie Thr His Phe G y y I I e Thr Ser 145 150 155 160 Su Ile Leu Asn Thr Asp Ala u y Arg Leu Ile Leu Ala Asp Ala 165 170 175 lie Ala Tyr Ala Ser G u Asp Lys Pro Asp Tyr Leu Ile Asp Ala Ala 180 185 190 Thr Leu Thr Gy Ala G n Leu Val Ala Leu y Leu Arg Thr Ser y 195 200 205 Val IVet G y Thr Asp G u Phe Arg Asp Ser Val Al a Lys Thr G y Arg 210 215 220 Su Val Gy u n Ala Trp Ala bet Pro Leu Pro u u Leu Asp 225 230 235 240 G u G n Val Lys Ser Pro Val Ala Asp Leu Arg Asn Val Thr Asn Ser 245 250 255 Arg Phe Ala Gy IVet Ser Ala Ala y Arg Tyr Leu n G u Phe Val 260 265 270 G y Ala Asp lie G u Trp Ala His Val Asp IIe Ala G y Pro Ala Tyr 275 280 285 Asn Thr Ala Gy G u Phe y Tyr Thr Pro Lys Arg Ala Thr Gy G n 290 295 300 Pro Val Arg Thr Phe Val G n Val Leu Lys Asp Leu Ser u Ser 305 310 315 Page 153 seql i st cor r ect ed. t xt <210> 115 <211> 980 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> FRXA01181 <400> 115 tct gta ct g Ser Val Leu 1 tac cca gag Tyr Pro G u ggc ttg cag G y Leu G n ttc ggc ggc Phe G y G y ctg ctg cgc Leu Leu Arg ttg gtt ggc Leu Val G y cct ggc gca Pr o G y Al a tcc gta ttg Ser Val Leu 115 aac gtc ttc Asn Val Phe 130 gct ttc cgc Al a Phe Ar g 145 gaa atc ttg Su Ile Leu att gct tac I le Al a Tyr acc ct g act Thr Leu Thr 195 gtc atg ggt Val MVt G y 210 gct cgc Al a Ar g 5 tac tca Tyr Ser acc at c Thr Ile ctc gca Leu Al a gat tgg Asp Trp 70 ggc at c G y lie atg gag et Q u acc at t Thr Ile ttc cta Phe Leu ggc gat Sy Asp 150 acc gac Thr Asp 165 tct gaa Ser G u gct caa Al a G n gat gag Asp u gac ttg gtg Asp Leu Val aac acc cct Asn Thr Pro t ca t ca cac ct g Ser Ser Hi s Leu 10 gt a Val ct g Leu gt c Val 55 aag Lys acc Thr aac Asn at c SIle cca Pr o 135 gt c Val gct Al a gat Asp tta Leu ttc Phe 215 gca Al a gag G u aac Asn cgc Ar g gac Asp at c SIle 105 gca Al a gct Al a act Thr ggc G y cct Pr o 185 gct Al a gac Asp aac Asn cag G n t cc Ser gct Al a 75 ggc G y gac Asp cgt Ar g aac Asn ttc Phe 155 ct c Leu t ac Tyr ggc G y gtt Val gaa G u ctt Leu t cc Ser aag Lys gga G y at g Met ttg Leu at g Met 140 ggt G y at t SIle ct c Leu ct g Leu gcc Al a 220 gcg Al a gct Al a cgc Ar g aag Lys at t SIle ggt G y aac Asn 125 cca Pr o ggt G y ct g Leu at t SIle cgg Ar g 205 aag Lys t cc Ser gat Asp aag Lys tcg Ser t cc Ser gga G y 110 ct g Leu t cc Ser at c SIle gca Al a gat Asp 190 act Thr act Thr aag Lys caa G n cct Pr o at c SIle at c SIle t cc Ser tcg Ser ggt G y acc Thr gat Asp 175 gcg Al a t ca Ser ggc G y cac Hi s ggt G y cgt Ar g gct Al a aag Lys gca Al a at c SIe gac Asp t cc Ser 160 gcc Al a gca Al a ggt G y cgc Ar g gag gtt ggc gag caa gca tgg gca atg cct ctt cct gaa gag ctc gat Page 154 seql i st cor r ect ed. t xt Su Val Gy u n Ala Trp Ala Met Pro Leu Pro u 225 230 235 gag cag gtt aag tcc cct gtc gct gac ctg cgc aat gtc G u G n Val Lys Ser Pro Val Ala Asp Leu Arg Asn Val 245 250 cgt ttc gca gga atg tct gct gcg ggt cgt tac ttg cag Arg Phe Ala Gy IVet Ser Ala Ala y Arg Tyr Leu G n 260 265 ggt gcc gac atc gag tgg gct cac gtc gat atc gct ggc G y Ala Asp lie G u Trp Ala His Val Asp Ile Al a G y 275 280 285 aac act gct ggt gaa ttc ggt tac acg cca aag cgc gca Asn Thr Ala Gy G u Phe y Tyr Thr Pro Lys Arg Al a 290 295 300 cca gtg cgc acc ttc gtt cag gtt ctg aag gat ctg tcg Pro Val Arg Thr Phe Val G n Val Leu Lys Asp Leu Ser 305 310 315 taaacgctag ttaaagatca gga <210> 116 <211> 319 <212> PRT <213> Cor ynebact er i um gl ut ai cum G u acc Thr gaa G u 270 cct Pr o acc Thr gaa G u Leu aat Asn 255 ttc Phe gca Al a gga Gy agc Ser <400> 116 Ser Val Leu Leu Ala Arg Asp Leu Val 1 Tyr Gy Phe Leu Leu Pr o Ser Asn Al a 145 G u G u G n Gy Ar g Gy Al a Leu 115 Phe Ar g Leu 5 Tyr Thr Leu Asp Gy bet Thr Phe Gy Thr 165 Ser SIle Al a Tr p 70 I le G u SIle Leu Asp 150 Asp Al a G u Asn Ar g Asp SIle 105 Al a Al a Thr Gy Thr Asn G n Ser Al a Gy Asp Ar g Asn Phe 155 Leu Ser Al a Al a Ar g Lys SIle Gy Asn 125 Pr o Gy Leu Ser Ser Asp Lys Ser Ser Gy 110 Leu Ser SIle Al a Hi s Lys G n Pr o SIe SIe Ser Ser Gy Thr Asp 175 Ile Ala Tyr Ala Ser u Asp Lys Pro Asp Tyr Leu Ile Asp Al a Al a 190 Page 155 seqI i st car r ec Thr Leu Thr G31y Ala O31n Leu Val Ala Leu (31y 195 200 Val I'b t G31y Thr As p O31u Phe Ar g Asp Ser Val 210 215 O31u Val G31y 31u O31n Ala Tr p Ala I'b t Pr o Leu 225 230 235 O31u O31n Val Lys Ser Pr o Val Ala Asp Leu Ar g 245 250 Ar g Phe Ala 31y I'b t Ser Ala Ala G31y Ar g Tyr 260 265 31y Ala Asp Ile(1u Tr pAa Hi sVal Asp Ilie 275 280 Asn Thr Ala 31y O31u Phe G31y Tyr Thr Pr o Lys 290 295 Pr o Val Ar g Thr Phe Val 03 n Val Leu Lys Asp 305 310 315 <210> 117 <211> 2127 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221> CIDS <222> (2104) <223> RXN01277 <400> 117 t act act cgg t tcacgt t ta cgt cggct ga t ccaat tgga t aaaaaacct gccggt caaa agat cact aa cct gaact t c ted. t xt Leu Arg 205 A a Lys 220 Pro O31u As n Val Leu 03 n A a 03 y 285 Ar g Ala 300 Leu Ser Ser G31y Le u As n 255 Ph e A a G31y Ser ggcgccct cg gaagccgcct tt c Ph e t gg Tr p ct g Le u t ac Tyr c ag O31n agt Ser g aa O31u ggg G31y gcc A a ag c Ser ccg Pr o c ag O31n at t lIe ag c Ser ct g Le u cgc Ar g gga O31y gag O31u ac c Thr g aa O31u gct A a gag O31u t gg Tr p g ac As p g aa O31u ct a Le u tt g Le u ct g Le u ac g Thr ct c Le u gcg A a 15 aaa Ly s g ac As p t ac Tyr ac c Thr at t I Ie 95 ct c Le u ag c Ser ac c Thr aac As n ac g Thr 80 g at As p at g act I'b t Thr 1 gcg t gg Ala Tr p cca gcc Pr o Ala gat gat As p As p 50 t tt tg g Phe Tr p 65 ttg gag Leu 03 u gt g gat Val As p gcg A a aag Ly s 35 cgc Ar g cgg Ar g t cg Ser gcg A a g aa O31u 20 g ac As p at t I Ie g ac As p t at Tyr tt g Le u 1 00 gat t ac acg Asp Tyr Thr aaa Ly s gcc A a gcc A a gcg A a g aa O31u gcg A a gag gat gag ggc gaa aac tgg gta tgg aag ggc gcg gtt gtg cgc tcg 31u As p (1u (1y 1u As nTr pValI Tr pLy s(1y Aa ValI ValI Ar gSe r Page 156 seqi i st car r ect ed. t xt ccg Pr o gcg Al a g at As p 1 50 g at As p ac g Thr ccg Pr o gcg Al a gt g ValI 230 gag G31u gt g ValI at t I Ie ggc G31y ct g Le u 310 aat As n ac g Thr gt t ValI tt t Ph e 120 gt g ValI ccg Pr o g at As p t ct Ser gag G31u 200 cat Hi s ag g Ar g ggc G31y aag Ly s gca Al a 280 cgc Ar g gga G31y gt c ValI cat Hi s ac c Thr 360 cgg Ar g ag g Ar g g aa G31u tt g Le u 1 70 t ac Tyr gcg Al a t gg Tr p tt g Le u gt c ValI 250 t gg Tr p ggc G31y tt t Ph e gcc Al a ac a Thr 330 c ac Hi s ccg Pr o gcg Al a gag G31u t t g Le u 155 ct g Le u ccg Pr a gag G31u Ar g g at As p 235 aag Ly s at t I e t t g Le u cag (31n ac g Thr 315 gaa G31u at t I e t t g Le u tt g Le u tt t Ph e 140 aag Ly s gt g ValI gcg Al a tt g Le u g at As p 220 tt c Ph e ct t Le u tt t Ph e gga G31y cct Pr a 300 ac a Thr at c I Ie g ac As p g at As p 110 aag Ly s ct g Le u gcg Al a ac g Thr gt g ValI 1 tt t Ph e ac a Thr aat As n gt g ValI agt Ser 270 ct g Le u tt t Ph e aat As n ac a Thr cca Pr a 350 g at As p tt c Ph e gcc Al a aag Ly s g at As p 1 75 ct c Le u gag G31u cct Pr a t cg Ser ccg Pr a 255 cct Pr a ct g Le u ac g Thr tt c Ph e gt g ValI 335 gag G31u g aa (31u t cg Ser ac g Thr t cc Ser 1 60 ac c Thr ac g Thr ggg G31y ggt G31y gag G31u 240 ac c Thr cgg Ar g tt a Le u cct Pr a ct g Le u 320 ccg Pr a cat Hi s at t I Ie ggc G31y 130 gct Al a gt c ValI gag G31u aag Ly s cgt Ar g 210 gag G31u t cg Ser t gc cys g at As p gag G31u 290 gag G31u tt a Le u aat As n ac c Thr gt g ValI 370 gct Al a gt g ValI t gg Tr p t ca Ser 1 ggg G31y g at As p ac g Thr g aa G31u gt c ValI 260 gct Al a ct t Le u ac g Thr ct c Le u ccg Pr a 340 cat Hi s gca Al a g at As p g at As p gt t ValI 1 ct g Le u act Thr gt g ValI tt t Ph e ac c Thr 245 at t I Ie ggg G31y gag G31u t cg Ser ct t Le u 325 ac a Thr gt g ValI gcg Al a 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 agt ttc acc gaa gcg cca acg Ser Phe Thr Gu Ala Pro Thr t tg ct g cgt gcg gag ct g cct ggt gcg Leu Leu Ar g Al a G31u Leu Pro G31y Al a Page 157 seql i st cor r ect ed. t xt ctt Leu 390 gag G u t ac Tyr cac Hi s acc Thr aac Asn 470 acc Thr gcc Al a cgt Ar g t ac Tyr at g Met 550 t ac Tyr tcg Ser gca Al a gcg Al a t ac Tyr 630 cag G n gct Al a cgt Ar g at t SIle t ac Tyr 440 ggc Sy cgt Ar g ct g Leu ctt Leu gga Sy 520 gaa G u cgc Ar g aac Asn gt g Val gt g Val 600 ctt Leu gaa G u gcg Al a aag Lys cat Hi s 410 gga Sy ggc Sy gca Al a ggc Sy cgc Ar g 490 gag G u aac Asn ttc Phe cac Hi s gac Asp 570 gcg Al a acc Thr gct Al a acc Thr gt g Val aag Lys 395 tgg Tr p gcc Al a tt c Phe tgg Tr p ggt Sy 475 at g Met cgc Ar g ggt G y ggt G y acc Thr 555 gat Asp gt g Val t ca Ser caa G n gag G u 635 gaa G u 380 gcg Al a gca Al a ttc Phe gag G u ttg Leu 460 gaa G u aag Lys ggc G y ggt G y gcg Al a 540 tgg Tr p ccg Pr o gt g Val acc Thr gct Al a 620 ggc Sy tcg Ser ccg ttg cag Pro Leu G n acc tcg gcg Thr Ser Al a 415 gag gag gaa Su u u 430 gtt tcc ctt Val Ser Leu 445 gaa aag ggc G u Lys G y ttc ggt ccg Phe G y Pro gtg tgg gag Val Trp u 495 tac gca acg Tyr Al a Thr 510 ttg ctg aca Leu Leu Thr 525 gca gtt gt g Al a Val Val tca gcg ggt Ser Al a G y gag gaa cgg G u G u Ar g 575 ggc gtc gag Sy Val u 590 cgg gac gac Arg Asp Asp 605 ttg ctt gat Leu Leu Asp ggc cat gcc G y Hi s Al a ctg atc tac Leu Ile Tyr ttt Phe 400 gat Asp cca Pr o acc Thr t ac Tyr gaa G u 480 gat Asp ccg Pr o agt Ser cag G n gct Al a 560 gcg Al a aag Lys cgc Ar g gcg Al a ggc Sy 640 385 gaa G u gga Sy caa G n cca Pr o t ac Tyr 465 tgg Tr p cac Hi s gag G u ggc G y gt g Val 545 tcg Ser gt g Val cga Ar g gt c Val ggc Sy 625 gcg Al a aat Asn acg Thr aac Asn agc Ser 450 ttt Phe cat Hi s cgc Ar g cag G n gcg Al a 530 ccg Pr o tgg Tr p at t SIle at t SIle cac Hi s 610 cag G n gcg Al a gct Al a aag Lys acc Thr 435 cac Hi s gt g Val tcg Ser gcg Al a at t SIle 515 tta Leu ttg Leu at g Met gag G u t at Tyr 595 ccc Pr o gcc Al a gat Asp ggt G y at t SIle 420 ct g Leu t cc Ser gaa G u cag G n gt g Val 500 gcg Al a act Thr gct Al a gcg Al a cag G n 580 cca Pr o gcg Al a gt g Val aac Asn cag G n 405 ccg Pr o gt c Val ccg Pr o gcc Al a gca Al a 485 ct c Leu at t SIe cag G n gat Asp gag G u 565 t ac Tyr ccc Pr o cac Hi s gat Asp aag Lys 645 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 2035 2083 acc tgg atc gag aag act Thr Trp lie u Lys Thr Page 158 seqI i st car r ect ed. t xt 650 655 t tg gat cag cag ggt agc at t t aat acct at gat tat gcga agg L eu As p O31n O31n O31y Ser I Ie 665 <210> 118 <21 1> 668 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 660 2127 <400> 118 vb t A a Pr o As p Ph e Le u ValI A a Ar g A a 145 As p O31y Tr p Ser Ph e 225 Thr As p Thr Thr Asp Tyr Tr p Ala O31L 2C A a Lys Asr As p Ar g I E Tr p Ar g As r O3 u Ser Ty r As p A a LeL 1 OC Val Val Ar c 115 O3 y 03 y AE 130 A a Phe Val Val Thr Tr r O3 u 03 y Ser 1 8C Lys Ar g (31 1 95 Ar g O31n Asr 210 O31u Ar g Thr Ser Leu (3 L 0 ys Asp Val 26C Asp Phe A E 275 Thr Phe Leu Lys Tr p Ser A a Leu 03 u A a Tyr Val 55 A a G n Hi s 70 O3 u Ser Asp A a 03 u Asp Ser Pr o O31u Asp A a Thr 135 Asp Asp Ser 150 Val Asp Leu 1 65 Leu Thr Asp Thr Pr o Leu Val A a Thr 215 Phe Val Ser 230 Thr 03 u 03 y 245 Ilie Val Lys G31y Ilie Pr o O31u G31y A a 40 Ser Pr o O31n O31u Ph e 120 ValI Pr o As p Ser O31u 200 Hi s Ar g O31y Ly s A a 280 As p O31u Ar g Ar g Ar g Pr o O31y 1 As p I Ie Ph e Thr O31y 1 O31n A a Ser Le u O31n 265 O31y As p ValI Le u O31y ValI Tr p As n A a O31u Le u 1 Le u Pr a O31u Ar g As p 235 Ly s I Ie Le u Thr O31u A a O31u Tr p As p Tr p Le u Ph e 140 Ly s ValI A a Le u As p 220 Ph e Le u Ph e O31y Pr a Ly s A a Ly s Ar g ValI ValI ValI 125 As p O31u O31y Ar g Ph e 205 Ser Tyr As p ValI ValI 285 A a Ly s As p Tyr Thr lIe Ly s Ph e A a Ly s As p 1 Le u O31u Pr a Se r Pr a 255 Pr a Le u Lys O31u Phe Leu 31u O31y O31y Ar g Asp Phe O31n Pro Val Phe Thr Pro Page 159 seqI i st car r ect ed. t xt 295 300 Thr Lys Asn Phe Thr 03 u Ser Thr Ser 305 ValI Le u ValI Tr p O31u 385 O31u O31y O31n Pr o Tyr 465 Tr p Hi s O31u O31y ValI 545 Ser ValI Ar g ValI O31y 625 A a Thr As p A a 355 O31n Pr o A a Ly s Thr 435 Hi s ValI Ser A a I Ie 515 Le u Le u vb t O31u Tyr 595 Pr o A a As p Le u 325 Thr ValI A a A a O31n 405 Pr o ValI Pr a A a A a 485 Le u I Ie O31n As p O31u 565 Tyr Pr a Hi s As p Ly s 645 Le u 310 As n Thr ValI Ser Le u 390 O31u Tyr Hi s Thr As n 470 Thr A a Ar g Tyr vb t 550 Tyr Ser A a A a Tyr 630 O31n 03 n 03 y Leu A a Thr As n O31u A a Ph e 375 O31u Thr Ph e A a Ar g 455 Le u Ly s As p O31y Pr a 535 Le u O31y Pr a Le u Ar g 615 Tyr Thr Ser O31u 345 Ser O31u ValI O31n Thr 425 O31y I Ie O31y As n ValI 505 Ser A a Tyr Pr a O31n 585 Thr Ph e As n Ph e Thr 330 Hi s Pr a A a Ly s Hi s 410 O31y O31y A a O31y Ar g 490 O31u As n Ph e Hi s As p 570 A a Thr A a Thr 315 (31u I Ie Le u Pr a Ly s 395 Tr p A a Ph e Tr p O31y 475 vb t Ar g O31y O31y Thr 555 As p ValI Ser O31n O31u 635 I Ie As p As p Thr 380 A a A a Ph e O31u Le u 460 O31u Ly s O31y O31y A a 540 Tr p Pr a ValI Thr A a 620 O31y ValI Le u O31y 365 Le u Pr a Thr O31u ValI 445 O31u Ph e ValI Tyr Le u 525 A a Ser O31u O31y Ar g 605 Le u O31y Thr Pr a 350 As p Le u Le u Ser O31u 430 Ser Ly s O31y Tr p A a 510 Le u ValI A a O31u ValI 590 As p Le u Hi s ValI 335 O31u O31u Ar g O31n A a 415 O31u Le u O31y Pr a O31u 495 Thr Thr ValI O31y Ar g 575 O31u As p As p A a Le u 320 Pr o Hi s I Ie A a Ph e 400 As p Pr a Thr Tyr O31u 480 As p Pr a Ser O31n A a 560 A a Ly s Ar g A a O31y 640 Val Ou Ser Leu Ilie 650 Tyr Thr 655 Page 160 seqI i st car r ect ed. t xt Trp Ilie u Lys Thr Leu Asp On On Gy Ser Ilie 660 665 <210O> 119 <21 1> 1812 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1789) <223> FRXA01277 <400> 119 t act act cgg t tcacgt t ta cgt cggct ga t ccaat tgga t aaaaaacct gccggt caaa agat cact aa cct gaact t c ggcgccct cg gaagccgcct tt c Ph e t gg Tr p ct g Le u t ac Ty r c ag O31n agt Ser gag (31u ccg Pr o gcg A a g at As p 1 50 g at As p ac g Th r g aa (31u ggg G31y gcc A a ag c Ser ccg Pr o c ag O31n gag O31u tt t Ph e 120 gt g ValI ccg Pr o g at As p t ct Ser at t lIe ag c Ser ct g Le u cgc Ar g gga O31y gag O31u g aa O31u Ar g ag g Ar g g aa O31u tt g Le u 1 70 t ac Ty r ac c Th r g aa O31u gct A a gag O31u t gg Tr p g ac As p t gg Tr p tt g Le u tt t Ph e 140 aag Ly s gt g ValI gcg A a ccg Pr o aag Ly s gcg A a 45 aag Ly s cgc Ar g gt g ValI gt a ValI gt g ValI 125 g at As p gag O31u ggc O31y Ar g g aa O31u ct a Le u tt g Le u ct g Le u ac g Th r ct c Le u t gg Tr p 110 aag Ly s ct g Le u gcg A a ac g Th r gt g ValI 1 90 gcg A a 15 aaa Ly s g ac As p t ac Ty r ac c Th r at t I Ie 95 aag Ly s tt c Ph e gcc A a aag Ly s g at As p 1 75 ct c Le u ct c Le u ag c Ser ac c Th r aac As n ac g Th r 80 g at As p ggc O31y t cg Ser ac g Th r t cc Ser 1 60 ac c Th r ac g Th r at g act I'b t Thr 1 gcg t gg Ala Trrp cca gcc Pr o Ala gat gat As p Asrp 50 t tt tg g Phe Trrp 65 ttg gag Leu 03 u gt g gat Val Asrp gcg gtt A a Val Ar g (31 130 gct gct A a A a 145 gat gt c As p Val ggc gag O31yOL1 t gg aag Tr p Lys gcg A a aag Ly s 35 cgc Ar g cgg Ar g t cg Ser gcg A a gt g ValI 115 ggg O31y Ph e ac g Th r ggg O31y cgt Ar g 1 g aa O31u 20 g ac As p at t I Ie g ac As p t at Ty r tt g Le u 1 00 cgc Ar g gct A a gt g ValI t gg Tr p t ca Ser 1 ggg (31y aaa Ly s gcc A a gcc A a gcg A a g aa O31u gcg A a t cg Ser g at As p g at As p gt t ValI 1 ct g Le u act Th r g at As p t ac acg Tyr Thr Page 161 seql i st cor r ect ed. t xt ccg Pr o gcg Al a gt g Val 230 gag G u gt g Val at t SIle ggc Gy ct g Leu 310 aat Asn acg Thr gtt Val agt Ser ctt Leu 390 gag G u t ac Tyr cac Hi s acc Thr gag G u 200 cat Hi s agg Ar g ggc G y aag Lys gca Al a 280 cgc Ar g gga Gy gt c Val cat Hi s acc Thr 360 acc Thr gct Al a cgt Ar g at t SIle t ac Tyr 440 ggc G y gcg gag ttg ttc ttt Ala G u Leu Phe Phe 205 tgg cgg gat tca aca Trp Arg Asp Ser Thr 220 ttg gat ttc tat aat Leu Asp Phe Tyr Asn 235 gtc aag ctt gat gtg Val Lys Leu Asp Val 250 tgg att ttt gtg agt Trp Ile Phe Val Ser 270 ggc ttg gga gtg ctg Sy Leu y Val Leu 285 ttt cag cct gtg ttt Phe G n Pro Val Phe 300 gcc acg aca aag aat Ala Thr Thr Lys Asn 315 aca gaa atc gtc aca Thr G u lie Val Thr 330 cac att gac ctc cca His lie Asp Leu Pro 350 ccg ttg gat ggc gat Pro Leu Asp y Asp 365 gcg cca acg ttg ctg Ala Pro Thr Leu Leu 380 aag aag gcg ccg ttg Lys Lys Ala Pro Leu 395 cat tgg gca acc tcg His Trp Ala Thr Ser 410 gga gcc ttc gag gag G y Al a Phe G u G u 430 ggc ttc gag gtt tcc Gy Phe G u Val Ser 445 gca tgg ttg gaa aag Ala Trp Leu G u Lys 460 gag ggg G u Gy cct Pr o tcg Ser ccg Pr o 255 cct Pr o ct g Leu acg Thr ttc Phe gt g Val 335 gag G u gaa G u cgt Ar g cag G n gcg Al a 415 gaa G u ctt Leu ggt G y gag G u 240 acc Thr cgg Ar g tta Leu cct Pr o ct g Leu 320 ccg Pr o cat Hi s at t SIle gcg Al a ttt Phe 400 gat Asp cca Pr o acc Thr t cg cgt cag Ser Arg G n 210 ttt gag cgg Phe G u Ar g 225 acg tcg ct g Thr Ser Leu gat tgc gat Asp Cys Asp acg gat ttc Thr Asp Phe 275 aag gag ttc Lys G u Phe 290 act gag t cg Thr G u Ser 305 gtt t ta acg Val Leu Thr ct c aat gat Leu Asn Asp gtc acc gcg Val Thr Al a 355 tgg gtg cag Trp Val n 370 gag ct g cct G u Leu Pro 385 gaa aat gct G u Asn Al a gga acg aag G y Thr Lys caa aac acc G n Asn Thr 435 cca agc cac Pro Ser His 450 gat Asp acg Thr gaa G u gt c Val 260 gct Al a ctt Leu acg Thr ct c Leu ccg Pr o 340 cat Hi s gca Al a ggt G y ggt G y at t SIe 420 ct g Leu t cc Ser 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 ggc t ac t ac G y Tyr Tyr 465 Page 162 ttt gtg gaa gcc Phe Val G u Al a aac ctg cgt ggt ggc ggt gaa Asn Leu Arg G y G y G y G u 470 475 acc aag ctg aac cgc atg aag Thr Lys Leu Asn Arg IVet Lys 490 gcc gac ctt gtg gag cgc ggc Ala Asp Leu Val G u Arg y 505 cgt ggc gga tcc aac ggt ggt Arg G y Gy Ser Asn Gy y 520 tac cca gaa gca ttc ggt gcg Tyr Pro G u Ala Phe G y Ala 535 540 atg ttg cgc tat cac acc tgg lIet Leu Arg Tyr His Thr Trp 550 555 taggtgtcgg caaccatggg aac <210> 120 <211> 563 <212> PRT <213> Cor ynebact er i um gl ut a seql i st cor r ect ed. t xt ttc ggt ccg gaa tgg cat Phe G y Pro G u Trp Hi s 480 gtg tgg gag gat cac cgc Val Trp G u Asp His Arg 495 tac gca acg ccg gag cag Tyr Ala Thr Pro G u G n 510 ttg ctg aca agt ggc gcg Leu Leu Thr Ser G y Al a 525 530 gca gtt gtg cag gtg ccg Ala Val Val G n Val Pr o 545 tca gcg ggt acc tcg tgg Ser Ala y Thr Ser Trp 560 tcg Ser gcg Al a at t SIle 515 tta Leu ttg Leu at g let cag G n gt g Val 500 gcg Al a act Thr gct Al a gca Al a 485 ct c Leu at t SIe cag G n gat Asp 1555 1603 1651 1699 1747 1789 1812 Ti cum <400> 120 lIet Thr Asp Tyr 1 Al a Pr o Asp Phe Leu Val Al a Ar g Al a 145 Asp Al a Lys Ar g Ar g Ser Al a Val 115 Sy Phe Thr Thr Phe Leu G u Asp 5 Lys Trp Ser y G u Ala Leu G u Ala Arg 40 Ala Tyr Val Ser Arg Ala G n His Pro Arg 70 Su Ser Asp n Pro Al a G u Asp G u G y 105 Ser Pro G u Phe Asp 120 Asp Ala Thr Val lile 135 Asp Asp Ser Pro Phe 150 Val Asp Leu Asp Thr 165 Asp Val Leu Sy Val Tr p Asn Al a G u Leu 155 Leu Thr G u Al a G u Tr p Asp Tr p Leu Phe 140 Lys Val Pr o Lys Al a Lys Ar g Val Val Val 125 Asp G u Sy G u Leu Leu Leu Thr Leu Tr p 110 Lys Leu Al a Thr Al a Lys Asp Tyr Thr SIe Lys Phe Al a Lys Asp 175 Sy G u y Ser Leu Thr Asp Ser y Tyr Pro Ala Arg Val Leu Thr Page 163 seql i st cor r ect ed. t xt 185 Sn Al a u Leu Tr p Ser Phe 225 Thr Asp Thr Lys Thr 305 Val Leu Val Tr p G u 385 G u Gy G n Pr o Tyr 465 Tr p Hi s G u Gy Ar g 195 G n Ar g Leu Asp Phe 275 Phe Ser Thr Asp Al a 355 G n Pr o Al a Lys Thr 435 Hi s Val Ser Al a SIle 515 Leu Thr Pro Leu Val Phe Thr 245 I le Gy G u Ser Leu 325 Thr Val Al a Al a G n 405 Pr o Val Pr o Al a Al a 485 Leu SIle G n Al a Val 230 G u Val SIle Sy Leu 310 Asn Thr Val Ser Leu 390 G u Tyr Hi s Thr Asn 470 Thr Al a Ar g Tyr Thr 215 Ser Sy Lys Pr o Sy 295 G n Asn G u Al a Phe 375 G u Thr Phe Al a Ar g 455 Leu Lys Asp Sy Pr o 535 Al a Ser Leu G n 265 Sy Asp Leu Ser G u 345 Ser G u Val G n Thr 425 Sy SIle Sy Asn Val 505 Ser Al a Tr p Leu Val 250 Tr p Sy Phe Al a Thr 330 Hi s Pr o Al a Lys Hi s 410 Sy Sy Al a Sy Ar g 490 G u Asn Phe Ar g Asp 235 Lys SIle Leu G n Thr 315 G u SIle Leu Pr o Lys 395 Tr p Al a Phe Tr p Sy 475 Iat Ar g Sy Sy Asp 220 Phe Leu Phe Sy Pr o 300 Thr SIle Asp Asp Thr 380 Al a Al a Phe G u Leu 460 G u Lys Sy Sy Al a 540 Phe 205 Ser Tyr Asp Val Val 285 Val Lys Val Leu Sy 365 Leu Pr o Thr G u Val 445 G u Phe Val Tyr Leu 525 Al a 190 Phe G u G y Thr Pro y Asn Ser G u 240 Val Pro Thr 255 Ser Pro Arg 270 Leu Leu Leu Phe Thr Pro Asn Phe Leu 320 Thr Val Pro 335 Pro G u His 350 Asp G u Ile Leu Arg Ala Leu G n Phe 400 Ser Al a Asp 415 G u G u Pro 430 Ser Leu Thr Lys G y Tyr G y Pro G u 480 Trp u Asp 495 Al a Thr Pro 510 Leu Thr Ser Val Val G n Page 164 seql i st cor r ect ed. t xt Val Pro Leu Ala Asp IVet Leu Arg Tyr His Thr Trp Ser Al a y Thr 545 550 555 560 Ser Trp bet <210> <211> <212> <213> <220> <221> <222> <223> 121 526 DNA Corynebact er CDS (526) RXA01914 i um gl ut am cum <400> 121 ggttttgagg aatggct agg cttgttaaaa gttagtttca atttgatgcc tcccccaacc aaagcggaga cacaacttca acgagaggac tcagctttca tac agc aca aca gca cca acc aag gtg tcc aag Tyr Ser Thr Thr Ala Pro Thr Lys Val Ser Lys 15 gcg aaa aat Ala Lys Asn gat gcc act Asp Al a Thr ctt cca Leu Pro gtt cgt gga Val Arg G y aag att gat Lys I e Asp gtc gct gaa ctc Val Ala Gu Leu aag Lys ctc gaa aag aag Leu G u Lys Lys ttg cca aag Leu Pro Lys gat tcc atc Asp Ser Ile gcc atc atc gt c Ala lie Ile Val gcg Al a 45 att ttt gaa ggc Ile Phe Gu y gaa Su gaa ct c G u Leu gcc ggc ggc gaa Ala G y G y G u ctc gat ttc atc Leu Asp Phe Ile agt acc gag cag Ser Thr G u G n cag Sn gcc gac atc ctc Ala Asp Ile Leu cag ctc gaa gct G n Leu G u Al a ggc gca aag gcc Sy Ala Lys Ala gca aac agc atc Ala Asn Ser Ile acc Thr cgc gtc cca ggc Arg Val Pro G y gac gtt gcg cct Asp Val Ala Pro gtc att Val lie 100 gcg gtt ggt Ala Val G y cgc gct t cc Arg Ala Ser 120 ggc aag gct gat G y Lys Al a Asp ctt gac gac gag Leu Asp Asp G u acc ctc cgc Thr Leu Arg 115 gaa aat gtc G u Asn Val ggc acg gcg gcc G y Thr Al a Al a tcc ctc ggt ggt Ser Leu y y gcc acc Al a Thr 135 acc att ggc gat Thr Ile y Asp ttg gga ctt Leu G y Leu 140 <210> 122 <211> 142 <212> PRT <213> Corynebacteri um gl utam cum <400> 122 IVet Ala Lys Asn Ala Tyr Ser Thr Thr Ala Pro Thr Lys Val Ser Lys Page 165 seql i st cor r ec 1 5 10 Asp Ala Thr Leu Pro Val Arg Gy Thr Val Ala Lys Lys Leu Pro Lys Lys Ile Asp Ala lie lle 40 Gy u Asp Ser Ile Gu Leu Ala Gy Gy u 55 Phe Ser Thr G u G n G n Ala Asp Ile Leu Thr 70 Sy Ala Lys Ala Thr Al a Asn Ser I e Thr Arg 90 Val Ala Pro Val I e Al a Val y Leu y Lys 100 105 Asp G u Thr Leu Arg Arg Ala Ser y Thr Al a 115 120 Sy Phe u Asn Val Ala Thr Thr I e y Asp 130 135 <210> 123 <211> 1497 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1474) <223> RXA02048 <400> 123 gcgcggcgga taacaagcag accgcgtttg tggaatcgct agactttgga tcagcagggt agcatttaat acctatgatt ted. txt G u Leu Val Ala II e Leu G n Leu Val Pr o Al a Asp Al a Ar g 125 Leu G y 140 Leu Phe Phe Al a Thr Leu Leu t cc Ser aac Asn gt a Val t ac Tyr gt g Val gcg Al a ccg Pr o ggc Gy ccc Pr o gcg Al a aaa Lys ttc Phe cct Pr o cac Hi s ct g Leu gac Asp acc Thr cac Hi s ggt Gy at c SIle ct c Leu gcg Al a gcc Al a 75 gcc Al a aca Thr cga Ar g at g Met ct g Leu aaa Lys ggc Gy gat Asp t ac Tyr acc Thr ct g Leu acc Thr aaa Lys t cc tac Ser Tyr 15 gag ctt G u Leu gca acg Al a Thr gac ct c Asp Leu gcc ggc Al a G y 80 ctg cgc Leu Arg 95 Page 16E gatctacacc tggatcgaaa atg cga agg ctg cgc IVet Arg Arg Leu Arg 1 aca gga att gat ttc Thr G y lie Asp Phe gat ctc acc tac cgc Asp Leu Thr Tyr Arg ctg cac atg gat aat Leu His IVet Asp Asn ggc ggc agc ctg cgc Sy y Ser Leu Arg acc cac atc caa gtc Thr His Ile n Val atc acc ttc cgc aac Ile Thr Phe Arg Asn 100 seqI i st car r ect ed. t xt caa at c ccg gt t gac cag gaa t tt t ca On Ilie Pro Val Asp O1n Ou Phe Ser 1 05 aac As n ct c Le u t gg Tr p 1 50 C ac Hi s at c I Ie cgc Ar g g ac As p t at Tyr 230 tt t Ph e CCC Pr o g aa O31u gcc Al a C ac Hi s 310 t gg Tr p gca Al a ggC G31y cgc Ar g 120 aac As n CCC Pr o C ac Hi s aaa Ly s CCC Pr o 200 gt a ValI cct Pr o aaa Ly s cca Pr a cca Pr a 280 ggC G31y t gg Tr p aac As n gga G31y gag O31u 360 ct g Le u gcc Al a g ac As p g ac As p 1 70 gt c ValI gcc Al a ct g Le u ggg G31y gt c ValI 250 cgc Ar g g aa O31u gga G31y ggc G31y ggc G31y 330 aag Ly s ct g Le u ag c Ser gt c ValI 140 ac g Thr gga G31y ggc G31y t ac Tyr gt t ValI 220 gcg Al a at g vb t t ac Tyr c aa O31n t gg Tr p 300 t ca Ser gcc Al a gct Al a aag Ly s g aa G31u 125 gcc Al a ccc Pr a t ac Tyr ag c Ser ct c Le u 205 t cg Ser gcc Al a tt a Le u cgc Ar g ggc G31y 285 g aa O31u ct c Le u t gt cys gcg Al a g at As p 365 110 t gg Tr p gcc Al a g ac As p gcc Al a at g vb t 1 90 gca Al a g aa O31u tt g Le u g ac As p gt a ValI 270 ct c Le u cga Ar g ggc G31y t ac Ty r g aa O31u 350 at t I Ie ct c acc at c cgc t ac Leu Thr I Ie Ar g Ty r 115 ggc at g at c ggc t gg G31y N/t I Ie G31y Tr p 130 cag cca aac ggc gcg O31n Pr o Asn G31y Al a 145 gag aag gcg ctt ttc O3 u Lys Al a Leu Phe 1 gcc at t at c acc ggt Al a Il e Il e Thr 03 y 1 75 acc acc t gg cac t ac Thr Thr Tr p Hi s Tyr 1 gcc gt c cac gt c gga Al a Val Hi s Val G31y 210 t cg ggc gt t gt g gt g Ser 03 y Val Val Val 225 cgg gct cgg at t tt g Ar g Al a Ar g Ilie Leu 240 gcc tac gaa aaa ctc Al a Tyr 03 u Lys Leu 255 gt c at c acc gaa gac Val Ilie Thr O31u Asp 275 tcc agc ttc gga gcc Ser Ser Phe G31y Al a 290 ct C at c gcc cac gaa Leu Il e Al a Hi s 03 u 305 ct C gcc caa t gg aac Leu Ala O31n Tr p Asn 320 gcg gaa t gg ct C t gg Ala O31u Tr p Leu Tr p 335 agt gcg t tg gaa tt c Ser Al a Leu 03 u Phe 355 ttg ctg gcc aac ccc Leu Leu Ala Asn Pro 370 cgc Ar g g aa (31u ccg Pr a g ac As p g at As p 1 c aa O31n g aa (31u gag O31u gag O31u tt c Ph e 260 g aa O31u aac As n ct c Le u g ac As p tt t Ph e 340 t at Tyr G31y 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 Page 167 seql i st cor r ect ed. t xt aag gat atg ttc gac gac cgc gtc tac aag cgc ggc gct Lys Asp IVet Phe Asp Asp Arg Val Tyr Lys Arg Gy Al a 375 380 385 cat gca ttg cgg gaa ttg ctt ggc gat gat gca ttc ttc His Al a Leu Arg G u Leu Leu G y Asp Asp Al a Phe Phe 390 395 400 cgc tcc tac gtt gcc gaa ggc cga cac gga ctc gtt gaa Arg Ser Tyr Val Ala G u G y Arg His G y Leu Val G u 410 415 ctg aaa cga cac ctc tac gca gtc tcc aca gac cac gca Leu Lys Arg His Leu Tyr Ala Val Ser Thr Asp His Ala 425 430 gca gtg tgg cag tcc tgg ctt cgc gat ctg gag ttg ccg Ala Val Trp G n Ser Trp Leu Arg Asp Leu G u Leu Pro 440 445 450 tct ggt ggt ttg gac tagtgcgcta tctgacgctg gcc Ser G y G y Leu Asp 455 <210> 124 <211> 458 <212> PRT <213> Corynebacter i um gl ut ari cum ct g Leu aaa Lys ccc Pr o gct Al a 435 gag G u act Thr gct Al a cgc Ar g 420 tta Leu ttt Phe 1267 1315 1363 1411 1459 1497 <400> 124 IVet Arg Ar 1 Thr G y II Asp Leu T\ Leu His s G y G y S Thr His II Ile Thr P" I e Ar g T} 11 I e G y Tr 130 Asn Gy Al 145 Ala Leu P\ Ile Thr G g Leu e Asp hr Tyr bt Asp er Leu en e 3 n he Arg 100 yr Arg 15 rp u a Pro he Asp y Asp 180 Arg Ser 5 Phe Asn Arg Val Asn Tyr Arg Val 70 Val Ala Asn n Sy Asn G u Leu Ser Trp 150 Val Hi s 165 Leu II e Thr Pro Val Pro 10 Leu G y Phe Hi s 25 Ala Pro Asn Leu 40 Arg Ala Leu Asp G u Lys Val Thr Arg Phe Arg His 90 Ile Pro Val Asp 105 Pro Arg Pro Leu 120 Asp Asn G y Al a 135 Phe Pro Cys Asp Phe His Thr Asp 170 Ser Lys His Val 185 Gy I e Leu Al a Al a Al a G n Ar g Leu Asp 155 Asn Ser Ar g Ar g Gy Thr Gy Ar g Phe G u 125 Al a Pr o Tyr Ser Asp Tyr Thr Leu Thr Lys Ser 110 Tr p Al a Asp Al a Mvet 190 Ser G u Al a Asp Al a Leu Leu Gy G n G u Al a 175 Thr Trp His Tyr G n Ser Arg G u Pro IVet Ala Thr Tyr Leu Ala Ala Val Page 168 seqi i st car r ect ed. t xt 200 Thr Val Ser 205 Se r G3 u Se r G3 y Hi s ValI 225 Ar g (31u Thr Ph e A a 305 O31n Tr p Le u A a G31y 385 Ph e ValI Hi s Le u G31y ValI Le u Le u As p 275 A a (31u As n Tr p Ph e 355 Pr o Le u Ly s Pr o A a 435 O31u Tyr A a As p 245 G31y Le u Hi s Ser I Ie 325 O31u Ar g A a ValI ValI 405 As p As p Pr o As p Tyr 230 Ph e Pr o O31u A a Hi s 310 Tr p A a G31y Ly s Hi s 390 Ar g Le u A a Ser 215 ValI A a Tyr I Ie Thr 295 O31n Le u A a Le u As p 375 A a Ser Ly s ValI G31y 455 ValI O31n Ph e 265 Le u (31u Ph e (31u ValI 345 A a Ph e Ar g ValI Hi s 425 O31n Le u Leu 03 y Val 220 o3 y As p A a 235 Val As p [Vb t 250 Ar g Ser Ty r O3 u A a 03 n O31y Thr Tr p 300 O31y As n Ser 315 O3 y Phe A a 330 Lys Ser A a Leu Pro Lys Asp Asp Arg 380 O3 u Leu Leu 395 A a 03 u 03 y 410 Leu Tyr A a Ser Trp Leu Asp A a Le u Ar g O31y 285 O31u Le u cys A a As p 365 ValI O31y Ar g ValI Ar g 445 Ar g A a 255 ValI Ser Le u Le u A a 335 Ser Le u Ly s As p O31y 415 Thr Le u A a 240 Tyr I Ie Ser I Ie A a 320 O31u A a Le u Ar g A a 400 Le u As p O31u <210O> 125 <21 1> 906 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CDS <222> (883) <223> RXN00621 <400> 125 aaat gaat cc gggt tt t tca gt t tcggggt gcaaat caga at gt cgccaa t ggcgaacac acgagcgt gc agaagat gt g cgt gact aag at cgggggct at g t ct gaa cgc ct a 115 l'bt Ser O31u Ar g Leu 1 Page 169 IN seql i st cor r ect ed. t xt aac gct ccg caa gca cca atc cat ccc atc acc cga acc cac cac ggt 163 Asn Ala Pro G n Ala Pro IIe His Pro IIe Thr Arg Thr His His G y 15 att gat ttc gta gac aac tat gaa tgg ctg agg gat aaa gaa tcc caa 211 lie Asp Phe Val Asp Asn Tyr u Trp Leu Arg Asp Lys G u Ser G n 30 gaa acc ttg gac tac ctg gag gcg gag aat gcg ttc acc aag cag gag 259 Q u Thr Leu Asp Tyr Leu G u Ala G u Asn Ala Phe Thr Lys G n G u 45 CK1 act gaa cag cta gcc aca ctg cgg gac aac atc tat gaa gag att aag 307 0 Thr G u n Leu Ala Thr Leu Arg Asp Asn Ile Tyr G u u Ile Lys 00 55 60 Stca cgc gtt aaa gaa acc gac atg tcc atc cca gtg cgt gcc gga aag 355 C Ser Arg Val Lys G u Thr Asp IVet Ser Ile Pro Val Arg Ala y Lys 70 75 80 0 cac tgg tat tac tct cgc act gaa gaa ggc aag agc tac ggc tat tcc 403 C( His Trp Tyr Tyr Ser Arg Thr G u G u G y Lys Ser Tyr G y Tyr Ser 95 100 tgc cgc att cca gtg act gaa ggg tcg gat gca tgg acc cct cct gtt 451 Cys Arg Ile Pro Val Thr G u G y Ser Asp Al a Trp Thr Pro Pro Val 105 110 115 atc cct gag ggt gag cca gcg cag ggt gaa acc atc atc atg gat gcc 499 lie Pro u Gy u Pro Ala G n y u Thr lie lie IVet Asp Ala 120 125 130 aac gag ttg gca gaa ggc cac gaa ttc ttc tcc atg ggt gca tca tct 547 Asn G u Leu Ala G u G y His G u Phe Phe Ser IVet G y Ala Ser Ser 135 140 145 gtc acc acc tct ggc cgc tac ctt gcg tat tcc acc gat gtc acg ggc 595 Val Thr Thr Ser G y Arg Tyr Leu Ala Tyr Ser Thr Asp Val Thr G y 150 155 160 165 gaa gag cgc ttt acg ttg cgc atc aag gat cta gaa act ggc gag ctg 643 G u G u Arg Phe Thr Leu Arg lie Lys Asp Leu G u Thr Gy G u Leu 170 175 180 ctt cct gat acc ctg act ggc att ttc tac ggt gct act tgg gtg ggg 691 Leu Pro Asp Thr Leu Thr Gy Ile Phe Tyr Gy Ala Thr Trp Val y 185 190 195 gag gag tac ctc ttt tac cag cgc gtt gat gat gcg tgg cgt cca gat 739 G u G u Tyr Leu Phe Tyr G n Arg Val Asp Asp Ala Trp Arg Pro Asp 200 205 210 act gtg tgg cgc cac aag gtg ggt acc ccg gtt gaa gaa gac gtg ttg 787 Thr Val Trp Arg His Lys Val G y Thr Pro Val G u G u Asp Val Leu 215 220 225 gtg tac cac gag cct gat gaa cgt tat tcc acc tgg gtg ggc acc act 835 Val Tyr His G u Pro Asp G u Arg Tyr Ser Thr Trp Val G y Thr Thr 230 235 240 245 cgt tca gaa aaa gtt cat cct ttt tgg ttg cgc ctc caa gat cac ctc 883 Arg Ser G u Lys Val His Pro Phe Trp Leu Arg Leu G n Asp His Leu 250 255 260 tgaagtacgc gtgcttcctt tcg 906 <210> 126 Page 170 seqi i st car r ect ed. t xt <21 1> 261 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 126 N/t Ser 03 u Ar g Le u As n Ala Pr o (31n Ar g As p Ph e Tyr ValI Ser Tr p I Ie vb t 145 Thr O31u A a A a O31u 225 Tr p Le u Hi s O31u Ly s O31u A a G31y Pr a 115 vb t A a ValI G31y Tr p 1 95 Ar g As p G31y As p G31y O31n G31u Ly s Ly s Ser ValI A a Ser G31y 1 65 Le u G31y As p Le u Thr 245 Le u As p Thr G31u Ar g Tr p Ar g Pr a G31u 135 Thr G31u Pr a G31u ValI 215 Tyr Ser ValI As p Le u Ly s Tyr Pr a 1 G31y A a Ser Ph e Thr 1 Le u Ar g G31u Ly s A a 10 As p Tyr A a G31u Ser 90 ValI G31u G31u G31y Thr 1 70 Le u Ph e Hi s Pr a ValI 250 Pr o As n Le u Thr Thr Ar g Thr Pr a G31y Ar g 1 Le u Thr Tyr Ly s As p 235 Hi s Hi s G31u A a Ar g vb t O31u G31y O31n 125 O31u Le u I Ie I Ie Ar g 205 G31y Ar g Ph e lIe Le u As n As n G31y As p O31u Ph e Tyr As p 1 Tyr As p Pr a Ser Le u 255 <210O> 127 <21 1> 906 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 ODS <222> (883) <223> FRXA00621 <400> 127 Page 171 seql i st cor r ect ed. t xt aaatgaatcc gggtttttca gtttcggggt gcaaatcaga atgtcgccaa tggcgaacac acgagcgtgc agaagatgtg cgtgactaag atcgggggct atg tct gaa cgc cta IVet Ser G u Arg Leu aac Asn at t l Ie gaa G u act Thr t ca Ser cac Hi s tgc Cys at c SIle aac Asn gt c Val 150 gaa G u ctt Leu gag G u act Thr gt g Val 230 cgt Ar g ccg Pr o ttc Phe ttg Leu cag G n gtt Val t at Tyr at t SIle gag G u 120 ttg Leu acc Thr cgc Ar g gat Asp t ac Tyr 200 tgg Tr p cac Hi s gaa G u gca Al a gac Asp t ac Tyr gcc Al a gaa G u tct Ser gt g Val gag G u gaa G u ggc Gy acg Thr 170 ct g Leu ttt Phe cac Hi s cct Pr o gtt Val at c l Ie t at Tyr gag G u ct g Leu gac Asp act Thr gaa G u gcg Al a cac Hi s 140 t ac Tyr cgc Ar g ggc Gy cag G n gt g Val 220 gaa G u cct Pr o cat Hi s gaa G u gcg Al a 45 cgg Ar g at g vbt gaa G u ggg Gy cag G n 125 gaa G u ctt Leu at c SIle at t SIle cgc Ar g 205 ggt Gy cgt Ar g ttt Phe 1 ccc atc acc cga Pro Ile Thr Arg 15 tgg ctg agg gat Trp Leu Arg Asp gag aat gcg ttc G u Asn Ala Phe gac aac atc tat Asp Asn Ile Tyr t cc atc cca gt g Ser Ile Pro Val 80 gaa ggc aag agc G u G y Lys Ser 95 tcg gat gca tgg Ser Asp Ala Trp 110 ggt gaa acc at c Gy u Thr Ile ttc ttc tcc atg Phe Phe Ser IVet 145 gcg tat tcc acc Ala Tyr Ser Thr 160 aag gat cta gaa Lys Asp Leu G u 175 ttc tac ggt gct Phe Tyr G y Al a 190 gtt gat gat gcg Val Asp Asp Ala acc ccg gtt gaa Thr Pro Val G u 225 tat tcc acc tgg Tyr Ser Thr Trp 240 tgg ttg cgc ctc Trp Leu Arg Leu Page 172 acc Thr aaa Lys acc Thr gaa G u cgt Ar g t ac Tyr acc Thr at c SIle 130 ggt Gy gat Asp act Thr act Thr tgg Tr p 210 gaa G u gt g Val cac Hi s t cc Ser cag G n at t SIle gga Sy t at Tyr 100 cct Pr o gat Asp t ca Ser acg Thr gag G u 180 gt g Val cca Pr o gt g Val acc Thr ggt Sy caa G n gag G u aag Lys aag Lys t cc Ser gtt Val gcc Al a tct Ser ggc G y 165 ctg Leu ggg Sy gat Asp ttg Leu act Thr 245 caa gat cac ctc G n Asp His Leu seqI i st car r ect ed. t xt 250 255 t gaagt acgc gt gct tcct t t cg <210O> 128 <21 1> 261 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri curn 260 <400> 128 I'bt Ser G3 u Ar g As p Ph e Ty r ValI Ser Tr p I Ie Nibt 145 Th r G31u Al a Al a G31u 225 Tr p Le u Hi s G31u Ly s G31u Al a G31y Pr o 115 vb t Al a ValI G31y Tr p 1 95 Ar g As p G31y As p Arg Leu Asn Hi s G3 y IlIe Ser G3 n (3 u a3 n a3 u Thr Il e Lys Ser 70 a3 y Lys His Tyr Ser Cys 1 00 Pr o Val Ilie Asp Al a Asn Ser Ser Val 150 Thr a3 y a3 u 1 65 a3 u Leu Leu 1 80 Val a31ya(1u Pro Asp Thr Val Leu Val 230 Thr Thr Ar g 245 Hi s Leu Al a As p Thr a31u Ar g Tr p Ar g Pr o a31u 135 Thr a31u Pr o a31u ValI 215 Tyr Ser a31n ValI As p Le u Ly s Tyr Pr o 1 a31y Al a Ser Ph e Thr 1 Le u Ar g a31u Ly s Al a 10 As p Tyr Al a a31u Ser 90 ValI a31u a31u a31y Thr 1 70 Le u Ph e Hi s Pr a ValI 250 Pr a As n Le u Thr Thr Ar g Thr Pr a a31y Ar g 1 Le u Thr Tyr Ly s As p 235 Hi s Hi s a31u Al a Ar g vb t a31u a31y a31n 125 a31u Le u I Ie I Ie Ar g 205 a31y Ar g Ph e lIe Le u As n As n I Ie a31y As p a31u Ph e Tyr As p 1 Tyr As p Pr a Ser Le u 255 <210O> 129 <21 1> 1539 <212> DNA <21 3> Cor ynebact er i urn gi ut anm curn Page 173 seqi i st car r ect ed. t xt <220> <221> ODS <222> (101)..(1516) <223> RXN00622 <400> 129 ttttaccagc gcgttgatga tgcgtggcgt ccagatactg accccggt t g aagaagacgt gt t ggt gt ac cacgagcct g t gt ggcgcca caaggt gggt cct Pr o gcc Al a gag O31u gac Asp cac Hi s aag Lys gac Asp ggc O31y gac Asp t ac Tyr 150 cgc Ar g t gg Tr p ggc O31y gcg Al a t gg Tr p aag Lys acc Thr gat Asp gcc Al a aat Asn cgc Ar g agg Ar g 120 acc Thr gt c Val t ct Ser gaa O31u t ac Tyr 200 gat Asp cca Pr o acc Thr gag O31u gca Al a ggc O31y ttg Leu gag O31u ggc O31y ggc O31y t cg Ser gga O31y 170 ggc O31y ccg Pr o gcg Al a ct C Leu t ct Ser gt g Val gt c Val ccg Pr o 75 gac Asp ggt O31y gcg Al a gat Asp ggc O31y 155 t ca Ser gaa O31u t ca Ser cag O31n gt t Val gaa O31u ct g Leu gt a Val aac Asn gcg Al a gt c Val at c I Ie ttc Phe 140 gga O31y ttc Phe cgc Ar g gac Asp at t l Ie 220 cag O31n gt a Val at t l Ie 45 gac Asp ttt Phe ct g Leu gat Asp ggc O31y 125 caa O31n aac Asn acc Thr acg Thr t at Tyr 205 cca Pr o aaa Lys cgc Ar g ccg Pr a ggc G y t cg Ser gcg Al a acc Thr 110 cag O3n cag O3n cca Pr a acc Thr ct g Leu 190 gt g Val gt g Val aag Lys 15 gt g Val cgc Ar g t cc Ser gt g Val cca Pr a 95 t ac Tyr gt c Val ct g Leu gaa O31u ccg Pr a 175 ct g Leu gcc Al a t cc Ser ttc Phe ct t Leu gcg Al a gat Asp ggg O31y 80 ct C Leu cgc Ar g gcg Al a gaa O31u t gg Tr p 160 gcg Al a aag Lys t cc Ser ttg Leu at g lvb t 1 at c l Ie cct Pr a gag O31u at t I e t gg Tr p gt c Val gat Asp at c l Ie ttt Phe 145 gac Asp cag O31n cag O31n cga Ar g gt g Val 225 aac gtt att Asn Val l Ie ct t Leu ttc Phe ggt O31y t gg Tr p gct Al a gcg Al a ttc Phe at g lvb t 130 gac Asp gcc Al a ct g Leu cag O31n ttg Leu 210 cac Hi s ggt O31y cag O31n gaa O31u gt c Val gt c Val aag Lys 100 at c l Ie ct t Leu gaa O31u gt c Val aac Asn 180 gt g Val gt c Val acc Thr cca Pr a t gc Cys cca Pr a t ac Tyr aca Thr gac Asp gat Asp ct g Leu at c l Ie at c l Ie at t l Ie 165 t ac Tyr ct C Leu act Thr gac Asp Page 174 seql i st cor r ect ed. t xt ctg gat gta tcc aag ccc Leu Asp Val Ser Lys Pro 230 235 aac ccc acg ttg ctc tac ggc tat ggt tcc G y Tyr G y Ser Asn Pro Thr Leu Leu Tyr t ac gaa t ca Tyr G u Ser atg gat cgt Ibet Asp Arg gaa at g ggt G u vet y 280 aac acc ttc Asn Thr Phe 295 aag att tct Lys Ile Ser 310 ggc at g ct c G y Iet Leu gcg atc gaa Al a lle G u atg ccg gaa bet Pro G u 360 cca ct c cac Pro Leu Hi s 375 tat gaa aac Tyr G u Asn 390 t cg ctc aac Ser Leu Asn gcg cag ctt Al a G n Leu gaa atg gt t G u Mvt Val 440 cgt gag act Arg G u Thr 455 gtg acc gaa Val Thr G u 470 <210> 130 <211> 472 <212> PRT att gat Ile Asp 250 atg at t Vet lie ggc tgg Sy Trp gac ttc Asp Phe cct gaa Pro G u 315 ggc gcc G y Al a 330 aac gt g Asn Val cca ct g Pro Leu aag gac Lys Asp gag gca G u Al a 395 acc cga Thr Arg 410 gcg act Al a Thr gga cac G y Hi s ttt gag Phe G u cca Pr o ttt Phe t ac Tyr at t SIle 300 at g Met at t l Ie cca Pr o acg Thr gt c Val 380 aag Lys gt g Val gca Al a ggc G y t ac Tyr 460 ggc Gy gcg Al a gac Asp 285 gat Asp ct g Leu gcc Al a ttc Phe gtt Val 365 t at Tyr aac Asn ttg Leu acc Thr ggt G y 445 ggc G y ttc Phe at t l Ie 270 aac Asn gtt Val gtt Val aac Asn gt c Val 350 at c SIle gaa G u t ac Tyr t ac Tyr ggt G y 430 gt g Val tgg Tr p tct Ser 255 gcc Al a ggc G y gcc Al a gca Al a at g Met 335 gat Asp gaa G u t ac Tyr ccc Pr o gt c Val 415 gga G y t ca Ser ttg Leu 240 at c SIle cac Hi s aaa Lys gac Asp gaa G u 320 gcc Al a ccg Pr o tgg Tr p at g Met aat Asn 400 gaa G u gaa G u gga G y at c SIle gcg Al a gtt Val acc Thr gcc Al a 305 ggc G y ggt Gy ct g Leu gat Asp gcg Al a 385 at c SIle cca Pr o ttc Phe cgc Ar g aac Asn 465 cgt Ar g cgt Ar g acc Thr 290 ct c Leu ggc G y gac Asp acc Thr gag G u 370 tcg Ser ttg Leu gcc Al a ctt Leu t ac Tyr 450 caa G n ttg Leu ggc G y 275 acg Thr at c SIle t ca Ser cgc Ar g tct Ser 355 tgg Tr p t at Tyr gcc Al a aaa Lys ct g Leu 435 gaa G u gca Al a t ca Ser 260 ggt G y aag Lys gag G u gct Al a ttc Phe 340 at g Met ggc Sy gcc Al a gt a Val tgg Tr p 420 aaa Lys aag Lys acc Thr 245 ct g Leu ggc G y aaa Lys cag G n ggt G y 325 aag Lys ct c Leu gat Asp cca Pr o aca Thr 405 gt a Val act Thr tgg Tr p ggt G y 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1539 taaaacttgt tcgactagcg aac Page 175 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut ari cum <400> 130 Mvt Asn Val lie Pro Pro Gy Trp Ala 1 I e Pr o G u SIle Tr p Val Asp SIle Phe 145 Asp G n G n Ar g Val 225 Tyr Al a Val Thr Al a 305 Gy Phe Asp Val Leu Gy Hi s SIle 115 Lys G u Pr o Phe G u 195 Tr p Ar g Tyr Leu Gy 275 Thr l Ie Ser 5 Cys Pr o Tyr Thr Asp Asp Leu SIle SIle SIle 165 Tyr Leu Thr Asp Ser 245 Leu Gy Lys G n Gy 325 Al a G u Asp Hi s 70 Lys Asp Gy Asp Ty r 150 Ar g Tr p Gy Al a Leu 230 Ty r bet G u Asn Lys 310 Gy Ser Gy Val Asn Leu Val Tyr Gy 135 Thr Leu SIle Gy Lys 215 Asp G u Asp et Thr 295 I le bet I e Pr o Hi s G u Ser SIle 105 Ser Phe Al a Tyr Ser 185 Lys Gy Ser Ser Gy 265 Ar g Thr Al a bet Leu Ser Val Val Pr o 75 Asp Gy Al a Asp Gy 155 Ser G u Ser G n Pr o 235 Asp SIle Tr p Phe G u 315 Al a G n Val SIle Asp Phe Leu Asp Gy 125 G n Asn Thr Thr Tyr 205 Pr o Pr o Sy Al a Asp 285 Asp Leu Al a Lys Val Ar g Ser Val Pr o Tyr Val Leu G u Pr o 175 Leu Al a Ser Leu Ser 255 Al a Sy Al a Al a bet 335 Phe Leu Al a Asp Sy Leu Ar g Al a G u Tr p 160 Al a Lys Ser Leu Leu 240 I e Hi s Lys Asp G u 320 Al a Sy Asp Arg Phe Lys Ala lie G u Ala Asn Val 340 345 Pro Phe Val Asp Pro 350 Page 176 seqI i st car r ec Leu Thr Ser I'bt Leu I'bt Pro G u Leu Pro Leu 355 360 As p G3 u Tr p G3 y As p Pr o Leu Hi s Asp Lys As p 370 375 Ala Ser Ty r Ala Pr o Ty r (31u Asn I Ie (31u Ala 385 390 395 Ilie Leu Ala Val Thr Ser Leu Asn Asp Thr Ar g 405 410 Pr o Ala Lys Tr p Val Ala O31n Leu Ar g Ala Thr 420 425 Phe Leu Leu Lys Thr O31u I'b t Val Ala G31y Hi s 435 440 Ar g Tyr O31u Lys Tr p Ar g O31u Thr Ala Phe O31u 450 455 Asn O31n Ala Thr G31y Val Thr O31u 465 470 <210> 131 <211> 1539 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221> CIDS <222> (1516) <223> FRXA00622 <400> 131 tt t taccagc gcgt tgat ga t gcgt ggcgt ccagat act g accccggt t g aagaagacgt gt t ggt gt ac cacgagcct g ted. t xt Thr Val 365 Val Ty r 380 Lys As n Val Leu A a Thr G31YG(1Y 445 Ty r 03 y 460 (31u Tyr Pr a ValI 415 G31y Ser Le u t gt ggcgcca caaggt gggt cct Pr a gcc A a gag (31u g ac As p c ac Hi s aag t gg Tr p aag Lys5 ac c Thr g at As p gcc A a aat As n cca Pr a ac c Thr gag (31u gca A a ggc G31y Le u gt t ValI g aa (31u ct g Le u gt a ValI aac As n gcg A a c ag O31n gt a ValI at t I Ie 45 g ac As p tt t Ph e ct g Le u aaa Lys5 cgc Ar g ccg Pr a G31y t cg Ser gcg A a aag Lys5 15 gt g ValI cgc Ar g t cc Ser gt g ValI cca Pr a 95 tt c Ph e ct t Le u gcg A a g at As p ggg G31y 80 ct c Le u at g aac gtt [Vb t Asn Val 1 at c c tt tt t Il e Leu Phe cct t tc gac Pro Phe Asp 35 gag ggt gt g 03 u 03 y Val 50 at t t gg tt g Ilie Trp Leu 65 t gg gct ggc Tr p Ala G31y gt c gcg a Val Ala Hi s ggt G31y 20 c ag O31n g aa O31u gt c ValI gt c ValI aag Lys5 1 00 at t cca I Ie Pro t gc cys cca Pr a t ac Tyr ac a Thr g ac As p g at As p gac gt gcgc at t gag ggt gt cgat acc tac cgc gat t t cat cat cct g Page 177 Asp Val Arg lie Olu Gy Val Asp 1 05 eqi i st car r ect ed. t xt Thr Tyr Arg Asp Phe I I e I I e Leu 115 ggC G31y g ac As p t ac Tyr 1 50 cgc Ar g t gg Tr p ggC G31y gcg A a ct g Le u 230 t ac Tyr at g vb t g aa O31u aac As n aag Ly s 310 ggC G31y gcg A a at g vb t ag g Ar g 120 ac c Thr gt c ValI t ct Ser g aa O31u t ac Tyr 200 g at As p gt a ValI t ca Ser cgt Ar g ggt G31y 280 tt c Ph e t ct Ser ct c Le u g aa O31u g aa O31u 360 ggC G31y G31y t cg Ser gga G31y 1 70 ggC G31y ccg Pr o gcg A a aag Ly s at t I Ie 250 at g G31y g ac As p cct Pr o ggC G31y 330 aac As n cca Pr o gcg A a g at As p ggC G31y 155 t ca Ser gaa O31u t ca Ser cag O31n CCC Pr o 235 g at As p at t I Ie t gg Tr p t t c Ph e gaa O31u 315 gcc A a gt g ValI ct g Le u at c I Ie tt c Ph e 140 gga G31y tt c Ph e cgc Ar g g ac As p at t I Ie 220 aac As n cca Pr a tt t Ph e t ac Tyr at t I Ie 300 at g vb t at t I Ie cca Pr a ac g Thr 110 c ag O31n c ag O31n cca Pr a ac c Thr ct g Le u 1 90 gt g ValI gt g ValI ac g Thr tt c Ph e at t I Ie 270 aac As n gt t ValI gt t ValI aac As n gt c ValI 350 at c I Ie gt c ValI ct g Le u g aa O31u ccg Pr a 1 75 ct g Le u gcc A a t cc Ser tt g Le u t ct Ser 255 gcc A a ggC G31y gcc A a gca A a at g vb t 335 g at As p g aa O31u gcg A a g aa O31u t gg Tr p 1 gcg A a aag Ly s t cc Ser tt g Le u ct c Le u 240 at c I Ie c ac Hi s aaa Ly s g ac As p g aa O31u 320 gcc A a ccg Pr a t gg Tr p at c I Ie tt t Ph e 145 g ac As p C ag O31n C ag O31n cga Ar g gt g ValI 225 t ac Tyr gcg A a gt t ValI ac c Thr gcc A a 305 ggC O31y ggt O31y ct g Le u g at As p at g vb t 130 g ac As p gcc A a ct g Le u C ag O31n tt g Le u 210 C ac Hi s ggC O31y cgt Ar g cgt Ar g ac c Thr 290 ct c Le u ggC O31y g ac As p ac c Thr gag O31u 370 aag Ly s gag O31u ccc Pr a tt t Ph e g aa O31u 1 t gg Tr p cgc Ar g t at Tyr tt g Le u ggC O31y 275 ac g Thr at c I Ie t ca Ser cgc Ar g t ct Ser 355 t gg Tr p ct t Le u g aa O31u gt c ValI aac As n 1 gt g ValI gt c ValI ac c Thr ggt O31y t ca Ser 260 ggt O31y aag Ly s gag O31u gct A a tt c Ph e 340 at g (31y 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 cca ct ccac gat aag gac gt ct at gaa tac at ggcg tcg tat gcc cca Page 178 sec Pr o Leu His Asp Lys Asp Val Tyr C 375 380 tat gaa aac atc gag gca aag aact Tyr G3 u Asn I I e G3 u A a Lys Asn T 390 395 t cg ct c aac gac acc cga gt g t tg t Ser Leu Asn Asp Thr Arg Val Leu T 410 gcg cag ctt cgg gcg act gca acc g Ala O31n Leu Ar g Ala Thr Ala Thr C 425 4 gaa atg gtt gcc gga cac ggc ggt g O31u I'b t Val Ala G31y Hi s 31y Gy1 y 440 445 cgt gag act gca t tt gag t ac ggct Ar g O31u Thr Ala Phe O31u Tyr G31y T 455 460 gt g acc gaa t aaaact t gt t cgact agcg Val Thr O31u 470 <210> 132 <211> 472 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 132 I'bt Asn Val Ilie Pro Pro Gy Trp A 1 5 Ilie Leu Phe Gy Cys Ala Ser Lys 1 Pro Phe Asp On Pro Ou Gy Thr F 40 O31u G31y ValI Ou Tyr Asp Val Asp F- li1e Tr p Leu Val Thr Hi s As n Ala C 70 Tr p Ala G31y Val Asp Lys Leu Asn S Val Ala Hi s Lys Asp Asp Val Ar g I 100 1 Asp Phe Ilie Ilie Leu G31y Tyr Ar g S 115 120 Ilie I'b t Lys Leu Ilie Asp G31y Thr F 130 135 Phe Asp O31u O31u Ilie Tyr Thr Val P 145 150 Asp Ala Pr o Val Ilie Ar g Leu Ser T 1 65 lIi st cor r ect ed. t xt Su Tyr I'b t A a Se r 385 a c ccc aat at c tt g yr Pr o As n I Ie Leu 400 ac gt c gaa cca gcc y r Val O31u Pr o Ala 415 igt gga gaa t tc ct t Sy 03 y 03 u Phe Leu .30 it g t ca gga cgc t ac (al Ser G31y Ar g Tyr 450 gg t tg at c aac caa r p Leu Ilie Asn O31n 465 Iaac Tyr gcc A a aaa Lys5 ct g Le u 435 g aa O31u gca A a A a gt a ValI t gg Tr p 420 aaa aag ac c Th r 1315 1363 1411 1459 1 507 1 539 I a Pro 10 I e Thr ~ro O31u fi s A a ~u G31y ier Leu 90 1e O31u ier 03 y The 03 y Il a Ser yr 03 y 1 70 Le u Ser ValI ValI Pr o As p G31y A a As p G31y 1 Ser O31n ValI I Ie As p Ph e Le u As p G31y 125 O31n As n Thr ValI Ar g Ser ValI Pr o Tyr ValI Le u O31u Pr o 1 O31n Leu Phe Asn Tyr Tr p I Ie O31u Ser (31y (31u Ar g Thr Leu Leu Lys Page 179 seql i st cor r ect ed. t xt 185 Lys Pro Ser G nG Arg L 2 Val Hi 225 Tyr G Al a Ai Val Ai Thr TI 2! Al a L 305 3 y Q3 3 y A Leu TI Asp G 3 Al a Si 385 Il e L Pro Al Phe L Arg T! 4! Asn G 465 <210> <211> <212> <213> <220> <221> <222> <223> <400> G u 195 Tr p Ar g Tyr Leu Gy 275 Thr SIle Ser Ar g Ser 355 Tr p Tyr Al a Lys Leu 435 G u Al a Leu G y Thr Al a Asp Leu 230 Ser Tyr 245 Leu Ibet 3 y 3 u Lys Asn G n Lys 310 G y G y 325 Lys Al a Leu Ibet Asp Pro Pr o Ty r 390 Thr Ser 405 Val Ala Thr G u Trp Arg G y Val 470 Sy Lys 215 Asp G u Asp let Thr 295 I le let l Ie Pr o Leu 375 G u Leu G n et G u 455 Thr Gy Ser Ser Sy 265 Ar g Thr Al a let Al a 345 Leu Asp SIle Asp Ar g 425 Al a Al a Al a Lys SIle 250 lbet Sy Asp Pr o Sy 330 Asn Pr o Lys G u Thr 410 Al a Sy Phe Q n Pr o 235 Asp SIle Tr p Phe G u 315 Al a Val Leu Asp Al a 395 Ar g Thr Hi s G u Asp Tyr 205 lie Pro 220 Asn Pro Pro G y Phe Al a Tyr Asp 285 Ile Asp 300 IVet Leu I e Al a Pro Phe Thr Val 365 Val Tyr 380 Lys Asn Val Leu Al a Thr G y G y 445 Tyr G y 460 Val Ala Val Ser Thr Leu Phe Ser 255 l e Al a 270 Asn y Val Ala Val Ala Asn IVet 335 Val Asp 350 Ile u G u Tyr Tyr Pro Tyr Val 415 G y G y 430 Val Ser Trp Leu 133 1629 DNA Cor ynebact eri um gl ut ai cum CDS (1606) RXN00982 133 Page 180 seql i st cor r ect ed. t xt gaaaacaaac gtcct t gaag ccgtaatgcc ccgt t cgaca ataaaaaggg tagtagcagt tcttgccgcc tcgactgcgc ttagcccctt tttggtatca atg ccc act gca gca lMt Pro Thr Ala Ala gcg Al a gcc Al a ccc Pr o ggc Gy gat Asp at g Met ggc Gy ct c Leu at c SIle aac Asn 150 at c SIle cgc Ar g ccc Pr o aaa Lys acg Thr 230 gaa G u gct Al a ctt Leu aag Lys t at Tyr caa G n aca Thr ct g Leu 120 acc Thr tgg Tr p gga Gy cca Pr o ct g Leu 200 t cc Ser ggc Gy at c I e tgt Cys gat Asp ggc Gy ttc Phe t ac Tyr gtt Val acc Thr ggc Gy cgc Ar g t cc Ser 170 cac Hi s tgg Tr p aac Asn ggc Gy tgg Tr p agc Ser agc Ser at c I e ggc Gy ct c Leu tgc Cys gaa G u acc Thr 140 cgc Ar g gga Gy gac Asp ggc Gy ttc Phe 220 acc Thr gaa G u at c SIle gt g Val 45 ttc Phe agc Ser gtt Val gac Asp ggc G y 125 t cc Ser caa G n acc Thr aag Lys at c SIle 205 ttc Phe cca Pr o gaa G u gac Asp ggc Sy ggt Sy caa G n gca Al a aac Asn 110 gct Al a agc Ser gca Al a t ac Tyr gtt Val 190 at g Met acc Thr ct a Leu tgc Cys 15 gt g Val ttt Phe aac Asn t cc Ser gt g Val 95 at c SIle ttc Phe ct g Leu ctt Leu ct c Leu 175 gt c Val gcc Al a tgg Tr p gcc Al a cca Pr o ccc Pr o gt c Val t cc Ser at g let 80 cag G n gca Al a gtt Val acc Thr ggc G y 160 gga Sy ct c Leu t cc Ser gtt Val gt g Val 240 1 cct Pr o at g let aag Lys ggt Sy aac Asn cct Pr o cca Pr o aaa Lys acc Thr 145 gat Asp tcg Ser gat Asp caa G n gca Al a 225 t ac Tyr cag G n cac Hi s gt c Val ggc Sy tgg Tr p cgc Ar g gga G y gaa G u 130 gac Asp gac Asp gt c Val t cc Ser gaa G u 210 gaa G u caa G n gt a Val t at Tyr cct Pr o cct Pr o cca Pr o gga G y t ac Tyr 115 t cc Ser aac Asn aag Lys t ac Tyr gca Al a 195 cag G n aac Asn aac Asn gat Asp tct Ser gcc Al a ggt Sy gaa G u at g Met 100 gat Asp tgc Cys acc Thr at c SIle gcc Al a 180 at g Met ggc Sy aac Asn tgg Tr p att SIe gat Asp caa G n ggc Sy gcc Al a gtc Val ttc Phe gag G u gcc Al a t cc Ser 165 acc Thr gcg Al a t ac Tyr gac Asp t ca Ser 245 aac aag atc gtc gcc gaa acc gga Asn Lys Ile Val Ala u Thr y acc aac cca acc Thr Asn Pro Thr Page 181 gtt gct cca cca Val Ala Pro Pro seqi i st car r ect ed. t xt cca Pr o gca A a c ag O31n ag c Ser 310 t gg Tr p gt a ValI ag c Ser cca Pr o ggC O31y 390 t gc CYS c aa O31n ac c Thr gt g ValI aac As n 470 ac c Thr ggC O31y c aa O31n gca A a 280 ctt Le u ct g Le u at g vb t g ac As p aac As n 360 cca Pr o gt c ValI G31y gca A a t ac Tyr 440 ac c Thr gca A a gcc A a 250 ggC G31y at g vb t ac c Thr aac As n gca A a 330 ggC G31y c ag O31n gca A a g ac As p ac c Thr 410 c aa O31n ac c Thr aac As n aac As n t gg Tr p 490 g at As p at g vb t cag O31n gt c ValI 315 ggC O31y gac As p cgc Ar g at g vb t at t I Ie 395 cca Pr o cca Pr a cac Hi s gga O31y gac As p 475 gt c ValI gt c ValI ac c Thr ct c Le u 300 ac c Thr gcc A a g ac As p at g vb t gca A a 380 t ac Tyr ac a Thr ct a Le u aac As n cca Pr a 460 at t I Ie g aa O31u cca Pr a gcc A a 285 ct a Le u cgc Ar g at c I Ie cca Pr a gt c ValI 365 cgc Ar g t cc Ser ag c Ser ct c Le u gag O31u 445 gga O31y gt t ValI ggC O31y cca Pr a 270 ac c Thr aac As n gcc A a t ca Ser t ac Tyr 350 at g vb t at g vb t gt t ValI O31y ct c Le u 430 ct t Le u C ac Hi s gt g ValI aac As n 255 gca A a aac As n cct Pr a t ac Tyr O31y 335 gt c ValI t gc cys gcc A a aaa Ly s cgc Ar g 415 C ag O31n gcc A a ggC O31y g ac As p ac a Thr 495 tt c Ph e cca Pr a gga O31y at t I Ie 320 C aa O31n at c I Ie aac As n t gg Tr p tt c Ph e 400 C ag O31n gga O31y g ac As p C aa O31n t ac Tyr 480 ccc Pr a gca A a ac c Thr t cc Ser 305 cca Pr a ac a Thr g aa O31u g aa O31u ac a Thr 385 ag c Ser cca Pr a ac c Thr gcc A a t cc Ser 465 ct c Le u ac c Thr t gg Tr p t cc Ser 290 aac As n C ag O31n ccc Pr a ag c Ser aac As n 370 ag c Ser t cc Ser ac c Thr ag c Ser at g vb t 450 at c I Ie cgc Ar g cca Pr a gcc A a 275 gt g ValI C ag O31n cca Pr a at c I Ie at c I Ie 355 ac c Thr at g vb t gga O31y g ac As p g ac As p 435 aac As n ggc O31y ac c Thr at t I Ie 260 ggc O31y C aa O31n t ca Ser t ca Ser cct Pr a 340 aac As n gt c ValI gt c ValI C aa O31n gga O31y 420 cca Pr a gcc A a ggc O31y gga O31y ac g Thr 500 C aa O31n ct c Le u ct g Le u ac c Thr 325 g ac As p gcc A a gca A a ac c Thr gcc A a 405 t ct Ser C aa O31n C ac Hi s ac c Thr C ac Hi s 485 gct A a 931 979 1 027 1 075 1123 1171 1219 1267 1315 1363 1411 1459 1 507 1 555 1 603 1 629 t aat tgct tt ccact tagt a gat <210O> 134 Page 182 seqI i st car r ect ed. t xt <21 1> 502 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 134 N/t Pr o Thr Al a Al a Al a 03 n 03 u As n Pr a Ly s O31y As n Pr a Pr a Ly s Thr 145 As p Ser As p O31n Al a 225 Tyr Thr Al a Thr Ser 305 Pr a ValI Tyr Pr a Pr a Pr a O31y Tyr 115 Ser As n Ly s Tyr Al a 1 95 O31n As n As n Al a Al a 275 ValI O31n Pr a ie As p O31n O31y Al a ValI Ph e O31u Al a Ser 1 65 Thr Al a Tyr As p Ser 245 Pr a O31n Le u Le u Thr 325 Al a Pr a O31y As p 70 vb t O31y Le u I Ie As n 150 li1e Ar g Pr a Ly s Thr 230 As n Pr a Al a O31n Ser 310 Tr p Ser Ser O31u Al a Tyr Ser Ser O31y 135 As p Ph e Tyr Ser As n 215 Tyr Ly s Al a O31y O31y 295 Pr a Pr a Al a Le u 40 Ly s Tyr O31n Thr Le u 120 Thr Tr p O31y Pr a Le u 200 Ser Oy le O31n Al a 280 Le u Le u vb t O31n O31y Hi s Ser As n Pr a 1 05 Le u Pr a O31u Le u O31n 1 85 Al a Le u Le u ValI ValI 265 As p Al a Le u Le u Ar g Oy le Thr Ph e 75 As p As n Ar g Tyr ValI 1 55 Tyr Thr As n As p Thr 235 O31u As p vb t O31n ValI 315 Ou le ValI Ph e Ser ValI As p O31y 125 Ser O31n Thr Ly s I Ie 205 Ph e Pr a O31y Pr a Al a 285 Le u Ar g (31u As p O31y O31y O31n Al a As n 110 Al a Ser Al a Tyr ValI 1 vb t Thr Le u Thr Pr a 270 Thr As n Al a Cys Pr o Val Pr o Phe Val Asn Ser Ser I'b t Val O31n 1I e Al a Phe Val Leu Thr Leu 03 y 1 Leu 03 y 1 Val Leu Al a Ser Tr p Val Al a Val 240 As n Pr o 255 Al a Phe As n Pr o Pro O31y Ty r I Ie 320 O31y (1n 335 Al a 0 y Al a Il e Ser 330 Page 183 Thr G u G u Thr 385 Ser Pr o Thr Al a Ser 465 Leu Thr I e I e 355 Thr Met Sy Asp Asp 435 Asn Sy Thr SIle Asp Al a Al a Thr Al a 405 Ser G n Hi s Thr Hi s 485 Al a Thr Val Asp 375 Asp Ser Leu Pr o Val 455 G n Asp seql i st cor r ect ed. t xt Asp Thr Gy Asp Asp Pro 345 Asn Met G n Arg Met Val 360 365 Pro Val Ala IVet Ala Arg 380 Val Phe Asp lie Tyr Ser 395 Gy Ile Thr Pro Thr Ser 410 Ala Val G n Pro Leu Leu 425 Tyr Trp Thr His Asn G u 440 445 Thr Val Asn Gy Pro y 460 Ala lIe Asn Asp Ile Val 475 Ala Thr Trp Val G u y 490 Val Cys Al a Lys Ar g 415 G n Al a Gy Asp Thr 495 <210> 135 <211> 1114 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1114) <223> FRXA00977 <400> 135 gaaaacaaac gtccttgaag ccgtaatgcc ccg tcttgccgcc tcgactgcgc ttagcccctt ttt gcg Al a gcc Al a ccc Pr o ggc gty gat gaa G u gct Al a ctt Leu aag Lys t at at c I e tgt Cys gat Asp ggc Gcy ttc tgg Tr p agc Ser agc Ser at c I e ggc gaa G u at c SIle gt g Val 45 ttc Phe agc gaa G u gac Asp ggc Gy ggt Gay caa Ittcgaca ataaaaaggg tagtagcagt ggtatca atg ccc act gca gca IVet Pro Thr Ala Ala 1 tgc cca cct cag gta gat att Cys Pro Pro n Val Asp Ile 15 gtg ccc atg cac tat tct gat Val Pro Vet His Tyr Ser Asp ttt gtc aag gtc cct gcc caa Phe Val Lys Val Pro Ala G n aac tcc ggt ggc cct ggt ggc Asn Ser Gy Gy Pro Gy y tcc atg aac tgg cca gaa gcc Page 184 Asp at g lvb t ggc O31y ct C Leu at c l Ie aac Asn 150 at c l Ie cgc Ar g ccc Pr o aaa Lys acg Thr 230 aac Asn cca Pr o gca Al a cag O31n agc Ser 310 t gg Tr p Ala Tyr Ser Phe Phe y Se seqi i st car r ect ed. t xt r n Ser l'bt Asn Trp Pro 3u Ala caa O31n aca Thr ct g Leu 120 acc Thr t gg Tr p gga G31y cca Pr o ct g Leu 200 t cc Ser ggc G31y at c l Ie caa O31n gca Al a 280 ct t Leu ct g Leu at g lvb t t ac Tyr gt t Val acc Thr ggc O31y cgc Ar g t cc Ser 170 cac Hi s t gg Tr p aac Asn ggc O31y gcc Al a 250 ggc O31y at g acc Thr aac Asn gca Al a 330 ct C Leu t gc Cys gaa O31u acc Thr 140 cgc Ar g gga O31y gac Asp O31y t t ttc Phe 220 acc Thr acc Thr gt c Val acc Thr ct C Leu 300 acc Thr gcc Al a gt t Val gac Asp ggc O31y 125 t cc Ser caa O31n acc Thr aag Lys at c l Ie 205 ttc Phe cca Pr o gga O31y cca Pr a gcc Al a 285 ct a Leu cgc Ar g at c l Ie gca Al a aac Asn 110 gct Al a agc Ser gca Al a t ac Tyr gt t Val 190 at g lvb t acc Thr ct a Leu acc Thr cca Pr a 270 acc Thr aac Asn gcc Al a t ca Ser gt g Val 95 at c l Ie ttc Phe ct g Leu ct t Leu ct C Leu 175 gt c Val gcc Al a t gg Tr p gcc Al a aac Asn 255 gca Al a aac Asn cct Pr a t ac Tyr ggg 31y 335 Cag O31n gca Al a gt t Val acc Thr ggc O31y 160 gga O31y ct C Leu t cc Ser gt t Val gt g Val 240 cca Pr a ttc Phe cca Pr a gga O31y at t l Ie 320 Caa O31n cct Pr a cca Pr a aaa Lys acc Thr 145 gat Asp t cg Ser gat Asp Caa O31n gca Al a 225 t ac Tyr acc Thr gca Al a acc Thr t cc Ser 305 cca Pr a aca Thr cgc Ar g gga O31y gaa O31u 130 gac Asp gac Asp gt c Val t cc Ser g aa O31u 210 g aa O31u Caa O31n gt t Val t gg Tr p t cc Ser 290 aac Asn Cag O31n ccc Pr a gga O31y t ac Tyr 115 t cc Ser aac Asn aag Lys t ac Tyr gca Al a 195 Cag O31n aac Asn aac Asn gct Al a gcc Al a 275 gt g Val Cag O31n cca Pr a at g lvb t 100 gat Asp t gc Cys acc Thr at c l Ie gcc Al a 180 at g rvb t 99t ggc O31y aac Asn t gg Tr p cca Pr a 260 ggc O31y Caa O31n t ca Ser t ca Ser gt c Val ttc Phe gag O31u gcc Al a t cc Ser 165 acc Thr gcg Al a t ac Tyr gac Asp t ca Ser 245 cca Pr a Caa O31n ct C Leu ct g Leu acc Thr 325 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1114 Page 185 seqI i st car r ect ed. t xt <210O> 136 <21 1> 338 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 136 I'bt Pr o Thr Al a Al a Al a G3 n G3 u As n Pr a Ly s O31y As n Pr a Pr a Ly s Thr 145 As p Ser As p O31n Al a 225 Tyr Thr Al a Thr Ser 305 Pr a ValI Tyr Pr a Pr a Pr a O31y Tyr 115 Ser As n Ly s Tyr Al a 1 95 O31n As n As n Al a Al a 275 ValI O31n Pr a ie As p O31n O31y Al a ValI Ph e O31u Al a Ser 1 65 Thr Al a Tyr As p Ser 245 Pr a O31n Le u Le u Thr 325 Al a Pr a O31y As p 70 vb t O31y Le u I Ie As n 150 li1e Ar g Pr a Ly s Thr 230 As n Pr a Al a O31n Ser 310 Tr p Ser Ser O31u Al a Tyr Ser Ser O31y 135 As p Ph e Tyr Ser As n 215 Tyr Ly s Al a O31y O31y 295 Pr a Pr a Al a Le u 40 Ly s Tyr O31n Thr Le u 120 Thr Tr p O31y Pr a Le u 200 Ser Oy le O31n Al a 280 Le u Le u O31n O31y Hi s Ser As n Pr a 1 05 Le u Pr a O31u Le u O31n 1 85 Al a Le u Le u ValI ValI 265 As p Al a Le u Le u Ar g O31y Thr Ph e 75 As p As n Ar g Tyr ValI 1 55 Tyr Thr As n As p Thr 235 O31u As p vb t O31n ValI 315 Ou le ValI Ph e Ser ValI As p O31y 125 Ser O31n Thr Ly s I Ie 205 Ph e Pr a O31y Pr a Al a 285 Le u Ar g O31u As p O31y O31y O31n Al a As n 110 Al a Ser Al a Tyr ValI 1 vb t Thr Le u Thr Pr a 270 Thr As n Al a cys ValI Ph e As n Ser ValI lie Ph e Le u Le u Le u 1 ValI Al a Tr p Al a As n 255 Al a As n Pr a Tyr Pr a Pr a ValI Ser vb t O31n Al a ValI Thr O31y 1 O31y Le u Ser ValI ValI 240 Pr a Ph e Pr a Oy le 320 Al a(31y Al a Il e Ser 330 Page 186 (31y (1n 335 seqi i st car r ect ed. t xt Thr Pr o <210O> 137 <21 1> 269 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (246) <223> FRXA00982 <400> 137 t ct caa cta gca gt c caa cca cta ctc Ser O31n Leu Al a Val O31n Pr o Leu Leu 1 5 caa acc cca tac tgg acc cac aac gag O31n Thr Pr o Tyr Tr p Thr Hi s As n O31u 25 cac gt g gt c acc gt c aac gga cca gga Hi s Val Val Thr Val Asn G y Pr o G y 40 acc aac caa gca atc aac gac att gtt Thr Asn 03 n Al a Il e Asn Asp Il e Val 55 cac acc gac gcc acc tgg gtc gaa ggc Hi s Thr Asp Al a Thr Tr p Val O31u G31y 70 gct ggc t aat tgct tt ccact tagt a gat Al a 03 y c ag O31n gcc Al a ggc G y g ac As p ac a Thr gga O31y g ac As p c aa G n t ac Tyr ccc Pr o ac c Thr gcc Al a t cc Ser ct c Le u ac c Thr ag c Ser at g vb t at c I Ie cgc Ar g cca Pr o g ac As p aac As n ggc G y ac c Thr at t I Ie <210O> 138 <21 1> 82 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 138 Ser O31n Leu Al a Val O31n Pro Leu Leu Leu O31n O31y Thr Ser Asp Pro 1 5 10 O31n Th r Pr o Tyr Tr p Thr Hi s Asn O31u Leu Ala Asp Ala I'bt As n Al a 25 Hi s Val Val Thr Val Asn O31y Pro O31y His O31y O31n Ser IIe O31y O31y 40 Thr As n O31n Al a I Ie Asn Asp I Ie Val Val Asp Tyr Leu Ar g Th r O31y 55 Hi s Thr As p Al a Thr Tr pVal O31u O31y As n Thr Pro Thr Pr o I Ie Thr 70 75 Al a 03 y <210O> 139 <21 1> 1419 Page 187 seql i st car r ect ed. t xt <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1396) <223> RXA00152 <400> 139 gt cat tgat a t ccaaggcac gaccgcgat t gt at ggaaag caaat agt ac t ggccat tcc caact aaaac t ggagt aacg aagcct aaat tt t taacaat at g aca gga ct a at c 11! l'bt Thr 03 y Leu I I e ct c Le u at a I Ie ag c Ser gt g ValI tt c Ph e g at As p ggC O31y ct t Le u cgt Ar g ac c Th r 1 50 ccg Pr o cgt Ar g g ac As p at a I Ie ct g Le u ac c Th r cga Ar g ccg Pr o gt g ValI g ac As p g ac As p 120 gt g ValI t gg Tr p cca Pr o aag Lys5 aaa Lys5 200 tt c Ph e CCC Pr o ac c Th r cgc Ar g gct A a ac c Th r t ac Ty r gt c ValI aac As n ct g Le u at c I Ie 1 70 gcc A a gcc A a ct g Le u cag O31n gt t ValI gca A a gt t ValI 75 t t c Ph e at c I Ie ggt O31y cgc Ar g cgc Ar g 155 cag O31n acc Th r gaa O31u gt c ValI ggt O31y t ca Ser ag g Ar g at t I Ie c aa O31n gt c ValI O31y cgc Ar g 140 at c I Ie c aa O31n at t I Ie ggt O31y tt t Ph e g aa O31u ggt O31y 45 at c I Ie ac c Th r at c I Ie ggt O31y at g vb t 125 ct c Le u ag c Ser t cg Ser tt g Le u g aa O31u 205 gt c ValI gcc A a ggC O31y g ac As p c aa O31n aac As n gt g ValI 110 ac c Th r cgt Ar g cgt Ar g at g vb t ac c Th r 1 90 aag Lys5 gcc A a 15 gcc A a ct g Le u ac c Th r g ac As p g aa O31u 95 gag O31u ct g Le u ggc O31y gt g ValI g aa O31u 1 75 gca A a c aa O31n gt c ValI gt c ValI ac c Th r cgt Ar g aac As n cca Pr a c ag O31n g aa O31u gag O31u g aa O31u 1 aag Lys5 g aa O31u gcc A a gt g ValI g aa O31u ct g Le u cgc Ar g ac c Th r cgc Ar g t ct Ser ac c Th r 130 g at As p aag Lys5 at g vb t c ag O31n at c I Ie 210 at c I Ie cgc Ar g gt t ValI gt g ValI gt g ValI gcc A a gt a ValI 115 ct c Le u gca A a gca A a aag Ly s cgc Ar g 1 ct c Le u aag Ly s ct t Le u cca Pr a gt c ValI gcc A a at c I Ie 1 00 gca A a act Th r gca A a at t I Ie gca A a 1 g aa O31u c aa O31n gag ggt gaa aag cac gca tcc atc ctg aac gca gaa gca gaa cgc caa Page 188 seqI i st car r ect ed. t xt e L eu As n A a 03 u A O31u (1y 215 Ou Lys His Ala Ser 11 220 a O31u Ar g O31n gcg A a 230 gcg A a t ct Ser ct t Le u ag c Ser aag Ly s 310 g aa O31u ac C Thr gt g ValI aag Ly s aac As n 390 aac As n t cc Ser Cgg at c I Ie ggt G31y aag Ly s aag Ly s 280 tt c Ph e gca A a cgc Ar g t ca Ser aac As n 360 gca A a ac c Thr gcc A a C aa O31n cgc Ar g gcc A a 250 ac C Thr gca A a g at As p ggt G31y at c I Ie 330 cct Pr o cca Pr o cgc Ar g ac a Thr g aa O31u 410 gcc A a 235 cga Ar g cca Pr o gag O31u t ct Ser gt c ValI 315 gca A a gaa O31u gt c ValI gt t ValI caa O31n 395 t t c Ph e g aa (31u gca A a gag (31u G31y ct g Le u 300 tt c Ph e aac As n at c I Ie g at As p g aa (31u 380 cct Pr o cct Pr o ggt G31y at c I Ie gt t ValI aac As n 285 g aa (31u cgc Ar g gcc A a gca A a cca Pr a 365 cct Pr a gag (31u gca A a g aa (31u c aa O31n ctt Le u 270 gcc A a ggt O31y t ac Tyr g ac As p gca A a 350 g aa O31u g aa O31u gt t ValI cca Pr a cgc Ar g aag Lys5 255 gct A a tcc Ser tt t Ph e g aa O31u aac As n 335 gca A a CCC Pr a gca A a gag O31u c ag O31n 415 gca A a 240 gt c ValI t at Tyr aag Lys5 gcg A a cca Pr a 320 gt g ValI gt c ValI ggt O31y gt a ValI gca A a 400 gca A a 225 gca A a aac As n c aa O31n at g vb t aag Lys5 305 aac As n g aa O31u gcc A a gag O31u tt g Le u 385 gca A a aac As n cgc Ar g gca A a t ac Tyr t gg Tr p 290 c ag O31n ac c Thr g ac As p gca A a at c I Ie 370 gag O31u cct Pr a cct Pr a t ac Tyr gca A a ct c Le u 275 gt c ValI tt c Ph e gt g ValI t gg Tr p gca A a 355 ctt Le u gct A a cct Pr a g aa O31u ct C cag Leu 03 n 245 at c aag I e Lys 260 gaa aag O3 u Lys at c cca I Ie Pr o ggc gca O3 y A a gat gaa As p (1u 325 t tc tc c Phe Ser 340 aac gcc As n A a cc aag Ser Lys ttg caa Leu 03 n acc gca Thr A a 405 gat t ac Asp Tyr 420 835 883 931 979 1 027 1 075 1123 1171 1219 1267 1315 1363 cga gag aat cct Arg 0 uAsn Pro tac gga aac Tyr O31y As n 430 taatcaggca taagaaaagg 1416 1419 <210O> 140 <21 1> 432 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 140 I'bt Thr O31y Leu I Ie Leu Ala I Ie Val Phe Leu Val Phe Val Ala Val 1 5 10 Val Val I Ie Lys Ser lieAla Leu Ilie Pro O31n O31y O31u Ala Ala ValI Page 189 seql i st cor r ect ed. t xt Leu G y Ser Tyr Thr SIle Leu G u Leu G u SIle G u Leu 145 Leu G n Sy Lys G u 225 Al a Asn G n Mbet Lys 305 Asn G u Al a G u Ar g Val Val Val Al a Val 115 Leu Al a Al a Lys Ar g 195 Leu G u Tyr Al a Leu 275 Val Phe Val Tr p Al a 355 Leu Phe Ser I e Tyr Thr Ser Thr Asp 165 Asp Al a Al a G n G n 245 Lys Lys Pr o Al a G u 325 Ser Al a Lys Val Phe 70 Asp Sy Leu Ar g Thr 150 Pr o Ar g Asp G u Al a 230 Al a Ser Leu Ser Lys 310 G u Thr Val Lys Asp Pr o SIle Val Ar g Asp 135 Lys Pr o G u SIle Sy 215 Ibt G n Al a Pr o G n 295 Asp Thr G u Al a Thr 375 25 Arg Thr Val Ser y Val Arg Ala Arg Ile G n Ala Val lie Thr 75 Val Thr Phe G n Ile 90 Asn Tyr Ile Val y 105 Val Val y G y lbt 125 lie Asn Arg Arg Leu 140 Sy Leu Arg lie Ser 155 Ser lie n n Ser 170 Arg Ala Thr Ile Leu 185 Thr Ala G u G y G u 205 Lys His Ala Ser Ile 220 Leu Arg Ala G u G y 235 Su Ala Arg Ala lie 250 Leu Thr Pro G u Val 265 lie Ala G u G y Asn 285 Ser Asp Ser Leu G u 300 G u G y Val Phe Ar g 315 Asp lie Ala Asn Ala 330 Asp Pro u lie Ala 345 Lys Pro Val Asp Pro 365 Asp G n Asn Val 110 Thr Ar g Ar g vbt Thr 190 Lys Leu G u G n Leu 270 Al a Sy Tyr Asp Al a 350 G u Thr Asp G u G u Leu Sy Val G u 175 Al a G n Asn Ar g Lys 255 Al a Ser Phe G u Asn 335 Al a Pr o Ar g Asn Pr o G n G u G u G u 160 Lys G u Al a Al a Al a 240 Val Tyr Lys Al a Pr o 320 Val Val Sy G y Leu Thr Arg Arg Val Su Pro u Ala Val 380 Page 190 seql i st cor r ect ed. t xt Leu u Ala Leu n Asn y Thr Thr Thr n Pro Gu Val u Ala 385 390 395 400 Ala Pro Pro Thr Ala Asn Phe Ala n G u Phe Pro Ala Pro G n Ala 405 410 415 Asn Pro G u Asp Tyr Ser Asp G n His Arg G u Asn Pro Tyr G y Asn 420 425 430 <210> 141 <211> 1098 <212> DNA <213> Cor ynebact er i um gl ut ari cum <220> <221> CDS <222> (101)..(1075) <223> RXA02558 <400> 141 ccctcccgaa attagaaatc cccttttaat gtgatatcac cattcttttt cagcacccca gacttaaaag gagcaccacc t t t ctgt t aa act gat at ca atg agc acc ata gaa 11! Met Ser Thr Ile u gag G u gca Al a cca Pr o gga Sy gga Sy gtt Val cat Hi s cga Ar g t cc Ser t ac Tyr act Thr gt c Val gct Al a ttc Phe ctt Leu ttg Leu ct g Leu ggg Sy 120 agg Ar g ggc G y gga Sy at t SIle gca Al a gct Al a gtt Val gt c Val gt g Val tct Ser cga Ar g ccc Pr o gct Al a aat Asn ct g Leu caa G n acg Thr 75 acc Thr cag G n tt c Phe aac Asn at c SIle gt c Val gag G u ct c Leu t cc Ser gga Sy at a SIle ttc Phe gtt Val ttt Phe 140 aac Asn gcc Al a aag Lys gca Al a 45 at c SIle ct g Leu act Thr ttt Phe ccc Pr o 125 gaa G u at t SIle aca gaa Thr G u 15 aac gtg Asn Val atg att Vet Ile aac act Asn Thr att gcc I e Al a tcg gtg Ser Val 95 gga cga G y Arg 110 cca ct g Pro Leu acc aac Thr Asn gca gcg Al a Al a cca Pr o tgg Tr p gt g Val tta Leu agc Ser aag Lys t ac Tyr tct Ser gaa G u 1 gt g Val tct Ser ct c Leu gaa G u at c SIle gt g Val at c SIle gt g Val gcc Al a 145 gga G y ttg Leu at g Met aac Asn gt c Val gt c Val gga Sy acg Thr 130 aaa Lys gaa G u gca Al a gcc Al a ggc G y ttc Phe cct Pr o 100 ctg Leu aaa Lys aat Asn atc atc gtg tgg cag gta lle Ile Val Trp n Val Page 191 IND seql i st cor r ect ed. t xt 150 155 160 165 gcc gat act gca cag gct agc ttc tct gtg gag gat ttc gaa gag ttc 643 Ala Asp Thr Ala G n Al a Ser Phe Ser Val G u Asp Phe G u G u Phe 170 175 180 4 ctg cac cag cag gcc gag tcc gca ctg cgt cac gtg gca acc cag cac 691 Leu His Q n G n Ala Q u Ser Ala Leu Arg His Val Ala Thr G n His 185 190 195 ccc tat gat tcc cca gtt gat ggt cgt gtt t cc ttg cgt ggc gct acc 739 Pro Tyr Asp Ser Pro Val Asp y Arg Val Ser Leu Arg y Ala Thr C1 200 205 210 00 gat gag gtc agt gaa gaa ctc gca gat gag gtg gca caa cga gca gct 787 0 Asp u Val Ser G u u Leu Ala Asp u Val Ala n Arg Ala Ala 215 220 225 (N gtt gca ggt ctt gaa atc gtc gaa gcc cgc atc tct tcc ttg agc tac 835 0 Val Ala y Leu G u I e Val G u Ala Arg Ile Ser Ser Leu Ser Tyr S230 235 240 245 (N gca ccg gaa att gcc cag gcg atg ctg cag cgc cag cag gct tcc gcg 883 Ala Pro u Ile Ala n Ala IVet Leu n Arg Gn n Ala Ser Ala 250 255 260 att gtt gat gcc cgc gaa aag atc gtc gag ggc gct gtc acc atg gtg 931 Ile Val Asp Ala Arg u Lys Ile Val u y Ala Val Thr Vet Val 265 270 275 gaa acc gca ctt gac cag ctt gag caa cgt gaa att gtg gat ttg gat 979 G u Thr Ala Leu Asp G n Leu G u G n Arg G u Ile Val Asp Leu Asp 280 285 290 cca gag cga cgc gcc gcg atg gtt tcc aac ctg ttg gtt gtg ttg tgt 1027 Pro G u Arg Arg Ala Al a IVet Val Ser Asn Leu Leu Val Val Leu Cys 295 300 305 tcc gac acc aat gct cag cca atc gtc aac gcc ggt agc ctc tac caa 1075 Ser Asp Thr Asn Ala G n Pro Ile Val Asn Ala y Ser Leu Tyr G n 310 315 320 325 taagacaatg gcccgcaaac agg 1098 <210> 142 <211> 325 <212> PRT <213> Corynebact eri um gl ut am cum <400> 142 bet Ser Thr I le u u Arg Thr Pro G y Ala Val Ala Thr u Pro 1 5 10 Val y His O u y Ala Arg Val Ser lie Asn G u Lys Asn Val Trp 25 Ser Leu y Ala y Pro Ala Ala Phe Ala Leu Leu Ala ebt lie Val 40 Leu IVet lie Ala Ser G y Val Phe Phe Ala G n Ser Ile Asn Thr Leu 55 Su Asn Asp y y y Thr Leu Ala Val Thr y Leu lie Ala Ser 70 75 lie Val Val Phe Thr Val Ala Leu Val Val Thr lie Thr Ser Val Lys 90 Page 192 seql i st cor r ect ed. t xt Val Val Ser Pro G y His Thr Leu Thr Val 0 n Phe Phe G y Ar g Tyr 110 100 SIle Val Al a 145 I le Asp Val Leu Al a 225 Ser G n Al a SIle Leu 305 Gy Thr 115 Lys Val Tr p G u Thr 195 Sy Ar g Leu Al a Thr 275 Asp Val Leu Ar g Val Asp Val 165 Phe Hi s Thr Al a Tyr 245 Al a Val Asp Cys G n 325 Ar g Ser Tyr 150 Al a Leu Pr o Asp Val 230 Al a l Ie G u Pr o Ser 310 Thr Val 135 Asn Asp Hi s Tyr G u 215 Al a Pr o Val Thr G u 295 Asp Sy 120 Ar g Sy Thr G n Asp 200 Val Gy G u Asp Al a 280 Ar g Thr 105 Leu Val Asn Al a G n 185 Ser Ser Leu SIle Al a 265 Leu Ar g Asn Ser Ar g Pr o G n 170 Al a Pr o G u G u Al a 250 Ar g Asp Al a Al a Phe Asn SIle 155 Al a G u Val Su I e 235 G n G u G n Al a G n 315 Pr o 125 Su I e Phe Al a Sy 205 Al a G u et SIle G u 285 Val SIle Pr o Thr Al a Ser Leu 190 Ar g Asp Al a Leu Val 270 G n Ser Val Leu Asn Al a Val 175 Ar g Val G u Ar g G n 255 G u Ar g Asn Asn <210> 143 <211> 798 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(775) <223> RXA00500 <400> 143 caccagccag catgaacaca atggactt cg tgccagctca ggcgcagtac tgaagcacct tttcgatctg gcccacggcc gagaggtacg ctgattcctc gtg tta gta cta gcc 115 Val Leu Val Leu Ala 1 cta gac acc tca acc cct gac ctg atc gtc ggc gtc gtc gac tcc gac 163 Leu Asp Thr Ser Thr Pro Asp Leu Ile Val G y Val Val Asp Ser Asp 15 Page 193 seql i st cor r ect ed.t acc atc atc aaa aac acc gga aac acc Thr G y Asn Thr cgc gcc gaa Arg Al a G u aac gag cag ctc acg ccc acc Asn G u G n Leu Thr Pro Thr ttg agc ttt tca gat atc gac Leu Ser Phe Ser Asp Ile Asp 60 ccg ttc act gga ctt cga gta Pro Phe Thr y Leu Arg Val 75 gat gcc ctg gga atc cct gtc Asp Ala Leu Gy lie Pro Val gct cac aat att ggt gca cgc Al a His Asn lie G y Al a Arg 105 gat gcg cgc cgc cgt gaa atc Asp Ala Arg Arg Arg G u lie 120 cgt gat cag gga cca gat gtc Arg Asp n y Pro Asp Val 135 140 ggc gct gta gac acc att tcg Sy Ala Val Asp Thr I e Ser 150 155 cca gaa gaa ctc cag aat gtc Pro u u Leu n Asn Val 170 gca agc ttg gtg gca gtg gct Ala Ser Leu Val Ala Val Ala 185 gtt cct ctt tac ctg cgc cgc Val Pro Leu Tyr Leu Arg Arg 200 cct aaa tct gca gcc atc ccc Pro Lys Ser Ala Ala lle Pro 215 220 att cgagct a cgg <210> 144 <211> 225 <212> PRT <213> Cor ynebact er i um gl ut ar <400> 144 Val Leu Val Leu Ala Leu Asp 1 5 Val Val Asp Ser Asp Thr y Asp Thr Arg Ala His Asn G u Thr gt c Val 45 gcg Al a ggc Gy t at Tyr aac Asn t ac Tyr 125 at c SIle at t SIle acc Thr gat Asp cca Pr o 205 Ile Ile G u Asp xt acc Thr cag G n at c l Ie at g Met gga Gy at c SIle 110 tgg Tr p gca Al a cct Pr o at g Met ttc Phe 190 gat Asp aag Lys gt c Val gt g Val gt c Val 95 ccg Pr o gca Al a cca Pr o gag G u cat Hi s 175 agt Ser gcc Al a acg Thr gt g Val t cc Ser 80 tgc Cys cac Hi s acc Thr gca Al a cac Hi s 160 agc Ser gt g Val aaa Lys ctt ctc Leu Leu ggt tgc Sy Cys ggc gca G y Al a t ca ct c Ser Leu gca tta Al a Leu tac cgc Tyr Arg 130 aac at c Asn Il e 145 ctg gt g Leu Val ggc aaa G y Lys gaa cca G u Pro gaa cca G u Pro 210 t ca t gal Ser 225 cgc Ar g gac Asp ggc Gy gcg Al a gac Asp gtt Val 115 t cc Ser cag G n gaa G u cct Pr o caa G n 195 aaa gca Al a gcc Al a ccg Pr o ttc Phe gcg Al a 100 gcc Al a ggc Gy at c SIle aaa Lys gcc Al a 180 cca Pr o cca cac Hi s aac Asn gga G y ggt G y at c SIe act Thr gaa Gu agc Ser ct c Leu 165 ccc Pr o ttg Leu aaa Lys Pro Lys gag gtg gat ctt G u Val Asp Leu gt gaaca ri cum Thr Ser Thr Pro Asp Leu Ile Val y 10 Asn Thr Arg Ala u Thr lie lie u 25 G n Leu Thr Pro Thr Val G n Lys Thr Page 194 seql i st cor r ect ed. t xt Ala lie Val Val Leu Leu Asp Ala Asn Leu Ser Phe Ser Asp Ile 55 G y Cys G y Pro G y Pro Phe Thr G y Leu Arg 70 Sy Ala Ala Phe y Asp Ala Leu y lie Pro 90 Ser Leu Asp Ala lie Ala His Asn lie G y Al a 100 105 Ala Leu Val Ala Thr Asp Ala Arg Arg Arg G u 115 120 Tyr Arg Ser G y G u Arg Asp G n y Pro Asp 130 135 Asn lie n lie Ser y Ala Val Asp Thr Ile 145 150 155 Leu Val G u Lys Leu Pro G u G u Leu G n Asn 165 170 Sy Lys Pro Ala Pro Al a Ser Leu Val Ala Val 180 185 G u Pro G n Pro Leu Val Pro Leu Tyr Leu Arg 195 200 G u Pro Lys Pro Lys Pro Lys Ser Ala Ala lie 210 215 Ser 225 <210> 145 <211> 630 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(607) <223> RXA00501 <400> 145 tggaaccaca accattggtt cctctttacc tgcgccgccc caaaacctaa atctgcagcc atccccgagg tggatctttc Asp Val Val Ar g SIle Val 140 Ser Val Al a Ar g Pr o 220 Gy Tyr Asn Tyr 125 I e I e Thr Asp Pr o 205 G u Val Val Pr o Al a Pr o G u Hi s 175 Ser Al a Asp gag cta cgg gaa ctc cgc agg gaa gac G u Leu Arg G u Leu Arg Arg G u Asp gag caa atc ctg ttc cca ggt gat aac Su n lie Leu Phe Pro y Asp Asn 30 gcc gtg gag ttt tcc cac ccc acc aat Ala Val G u Phe Ser His Pro Thr Asn 45 gcg ggg Al a G y 15 ccc tgg Pro Trp ttc tac Phe Tyr Page 195 agatgccaaa gaaccaaaac atg agt gaa caa ttc IVet Ser G u G n Phe 1 cgc tgc gcc gac ctg Arg Cys Ala Asp Leu cca cgt gat gtc ttt Pro Arg Asp Val Phe atc ggc gct ttc gac ile G y Al a Phe Asp g aa (31u g at As p c aa O31n gcg Al a gt a ValI gt g ValI c aa (31n 1 50 ggg (31y t ac Tyr cca Pr o aaa Ly s ag c Ser gcg Al a 120 aaa Ly s cca Pr o ccc Pr o seqi i st car r ect ed. t xt gt g gcg t ac gca ggt ct t gcc at g at g Val Al a Ty r Ala G1y Leu Ala l'b t l'b t t tt gaa at c cac acc at t ggt gt c gat Phe (31u I Ie Hi s Thr I Ie G31y Val As p 75 80 t tg gga cgc gt a ct c at g gat caa at g Leu 03 y Ar g Val Leu l'b t As p O31n lb t 95 gac ggt cca gt t t tc t tg gaa gt c cgc Asp G31y Pr o Val Phe Leu O31u Val Ar g 110 t cc at g t ac gag gct t tc ggc t tt aaa Ser l'b t Ty r 03 u Al a Phe 03 y Phe Lys 125 130 t ac t ac cgg cca t cc gga gct gac gcc Tyr Tyr Ar g Pr o Ser G31y Al a Asp Al a 140 145 ttg agc gat cgc aaa gat caa cag aca Leu Ser Asp Ar g Lys Asp G31n G31n Thr 155 160 t aaaccat ga t cgtt t tggg aat gga G31y ccg Pr o at g vb t ac c Thr 115 ac c Thr t ac Tyr g ac As p cct Pr o g aa (31u cat Hi s 1 00 g ac As p tt g Le u ac c Thr ac a Thr gcg Al a tt c Ph e gca Al a aac As n gcc Al a at g vb t gag G u 1 <210O> 146 <211> 169 <21 2> PRT <21 3> Cor ynebact er i urn <400> 146 l'bt Ser 03 u 03 n Phe (3 1 5 Ar g Cy s Al a Asp Leu 03 Pro Arg Asp Val Phe Al 1 1 e 03 y Al a Phe As p 03 l'b t 'b t G31y Pr o Al a As Val As p Pr o O31u Phe 03 O31n Nib t Nibt Hi s Al a Al 1 00 Val Ar g Thr As p As n Ve 115 Phe Lys Thr Leu Al a Ve 130 As p Al a Ty r Thr N/bt (31 145 1 gl ut ani cumn u Leu Ar g O31u U a U n a n 30 O31n ValI G31y As p Ar g As p Pr a Ar g 135 Ar g I Ie O31u 40 Tyr Pr a Lys5 Ser Al a 120 Lys5 Pr a Le u 25 Ph e Le u O31u G31y Hi s 1 05 li1e As n Ar g Leu Arg Phe Pro Ser Hi s I Val Al a Phe 03 uI 75 Leu 03 y As p (1y I Ser l'b t Ty r Ty r Leu Ser 1 55 Page 196 O31u As p Thr Al a Hi s ValI ValI O31u 125 Pr a Ar g As p As n As n G31y Thr Le u Ph e 110 Al a Ser Lys5 G31y Tr p Tyr Al a G31y As p O31u G31y Al a (31n 1 seql i st cor r ect ed. t xt G n Thr Asp Thr G u G y Thr Pro Ser 165 <210> 147 <211> 1155 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(1132) <223> RXA00502 <400> 147 ctaccggcca tccggagctg acgcctacac catgcaacgc agatcaacag acagacacag aggggacacc cagctaaacc ccacgcttga gcgatcgcaa at t SIle ggc Gy caa G n gcg Al a gcg Al a ttg Leu gct Al a gt c Val ttg Leu gga Gy 150 gaa G u ggc Gy agc Ser gga Gy cat Hi s ct g Leu gt c Val ggc Gy tgg Tr p gt g Val 120 gtt Val ccc Pr o t at Tyr at c SIle tgc Cys ct a Leu cgc Ar g tct Ser agg Ar g ct g Leu gtt Val aat Asn ggc Gy aag Lys aaa Lys 170 gat Asp gat Asp gag G u ttt Phe at g bet cca Pr o 75 ct c Leu ccg Pr o ct g Leu gga Gy gaa G u 155 gt c Val aaa Lys gaa G u at c SIle ggt Gy gt c Val gat Asp gtt Val ttt Phe gaa G u cac Hi s 140 ttg Leu t ca Ser ttg Leu aca Thr ct c Leu ggc Gy 45 ccc Pr o gct Al a gga Gy t ac Tyr ggt Gy 125 act Thr gga Gy agg Ar g gcg Al a ggc gta ggc Sy Val y 15 gcc gac t ca Al a Asp Ser gtc gtg cca Val Val Pr o gtg atg cgt Val Vet Arg gtg gct gca Val Al a Al a 80 gcc agc gct Al a Ser Al a 95 gcg gtc aac Al a Val Asn 110 gaa act ct t G u Thr Leu caa ttg ttg G n Leu Leu t cc acc ct c Ser Thr Leu 160 ctg ttg gga Leu Leu G y 175 cgc cgg ggt Arg Arg G y atg atc gtt 'e t Ile Val 1 gt a gt c aaa Val Val Lys gtg gcc t cc Val Al a Ser gaa at c gcc G u Ile Al a gaa gcg ttg G u Al a Leu acc gtg ggc Thr Val G y gcg aag gcg Al a Lys Al a cac ctg ggc Hi s Leu G y 115 cca cac gcg Pro His Ala 130 gaa gtc gac G u Val Asp 145 gac gat gcc Asp Asp Al a ttg ggc tac Leu G y Tyr ttg gga Leu G y ctt gac Leu Asp t cc atg Ser bet tcc cgg Ser Arg agg cag Arg G n cct ggt Pro G y tat gcc Tyr Al a 100 gga cac G y Hi s gtg gct Val Ala gcg gtg Al a Val gct ggc Al a y 165 cca ggc Pro G y 180 aat cca gag gcc att Asn Pro Gu Ala lie Page 197 INO seql i st cor r ect ed. t xt 185 190 195 gct ttc ccc cgc gga ttg atg aaa aag tcg gat tct cgg cat gat ttc 739 Ala Phe Pro Arg G y Leu IVet Lys Lys Ser Asp Ser Arg His Asp Phe 200 205 210 4 agc ttt tcc ggt ttg aaa acc tcc gtt gcc cgc tac gtg gaa gct gcg 787 Ser Phe Ser y Leu Lys Thr Ser Val Ala Arg Tyr Val G u Ala Ala 215 220 225 gaa aga aac ggt gaa gtt att tcc gtg gag gac gtc tgc gca tca ttc 835 G u Arg Asn Gy G u Val Ile Ser Val G u Asp Val Cys Ala Ser Phe C 230 235 240 245 00 caa gaa gcg gtg tgt gat gtg ttg acg ttt aag gcc gtg cgt gcg tgc 883 G n G u Ala Val Cys Asp Val Leu Thr Phe Lys Ala Val Arg Ala Cys 250 255 260 (N cgc gat gtc ggt gcg aag gtg ctg ctg ttg ggt gga gga gtg gct gcc 931 0 Arg Asp Val y Ala Lys Val Leu Leu Leu G y Gy y Val Ala Ala 265 270 275 (N aac tct cgt ctg cgg gag ctt gct caa gaa cgt tgc gat aaa gcc gac 979 Asn Ser Arg Leu Arg G u Leu Ala n G u Arg Cys Asp Lys Ala Asp 280 285 290 atc gaa ctc cgg gtt cct cgt ttc aat ttg tgc acc gat aat ggt gtc 1027 Ile G u Leu Arg Val Pro Arg Phe Asn Leu Cys Thr Asp Asn y Val 295 300 305 atg att gca gcg ttg gcg gct caa aga atc cac gaa ggt gcc caa gaa 1075 IVet lle Ala Ala Leu Ala Al a n Arg lle His G u G y Al a Gn G u 310 315 320 325 tca cca att tcg gtc gga act gat cct tct ttg tcc gtt gag acc cca 1123 Ser Pro lie Ser Val G y Thr Asp Pro Ser Leu Ser Val G u Thr Pro 330 335 340 cag gtg ttt taaacattta gtattagttc cat 1155 G n Val Phe <210> 148 <211> 344 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 148 Met Ile Val Leu Gy Ile u Ser Ser Cys Asp u Thr y Val y 1 5 10 Val Val Lys Leu Asp G y G u Gy Asn Leu u Ile Leu Ala Asp Ser 25 Val Al a Ser Ser t G n G u Hi s Al a Arg Phe G y G y Val Val Pro 40 G u Ile Ala Ser Arg Al a His Leu u Ser IVet Val Pro Val IVet Arg 55 G u Ala Leu Arg G n Al a y Val Asp Arg Pro Asp Ala Val Ala Ala 70 75 Thr Val y Pro y Leu Ala y Ala Leu Leu Val y Ala Ser Ala 90 Ala Lys Ala Tyr Ala Al a Ala Trp y Val Pro Phe Tyr Ala Val Asn Page 198 seql i st cor r ect ed. t xt 105 Ala Asn Leu G u Hi s Pr o G u 145 Asp Leu Asn Ser Tyr 225 Val Al a Gy Cys Thr 305 G u Ser Gy 115 Al a Asp Al a Tyr G u 195 Hi s G u Al a Ar g Val 275 Lys Asn Al a G u Hi s Al a Val Gy 165 Gy I e Phe Al a Phe 245 Cys Al a Asp Val G u 325 Pr o Al a Leu 135 Leu Al a Pr o Phe Phe 215 Ar g G u Asp Ser G u 295 I le Pr o Val Val 120 Val Pr o Tyr SIle Pr o 200 Ser Asn Al a Val Ar g 280 Leu Al a SIle Phe Ser Met Asp SIle 185 Ar g Gy Gy Val Gy 265 Leu Ar g Al a Ser Gy G u 155 Val Lys Leu Lys Val 235 Asp Lys G u Pr o Al a 315 Gy Hi s 140 Leu Ser Leu Met Thr 220 I le Val Val Leu Ar g 300 Al a Thr Gy 125 Thr Gy Ar g Al a Lys 205 Ser Ser Leu Leu Al a 285 Phe G n Asp 110 G u Thr Leu G n Leu Leu Ser Thr Leu 160 Leu Leu G y 175 Arg Arg G y 190 Lys Ser Asp Val Al a Arg Val G u Asp 240 Thr Phe Lys 255 Leu Leu G y 270 Sn G u Arg Asn Leu Cys Arg Ile His 320 Pro Ser Leu 335 <210> 149 <211> 888 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(865) <223> RXN02589 <400> 149 gcctaaattg cagcgagagg tctaaaaggt agtgctctag ggattcatcc aaactcacga atattgaagt tttaaagttg aacaggaaaa ataacaaata atg tct att tct gat 115 IVet Ser lie Ser Asp 1 aat tcc cgc gat caa tta gga gaa ctg cca gct ggt cgg cct ctc caa 163 Asn Ser Arg Asp n Leu G y G u Leu Pro Ala Gy Arg Pro Leu G n 15 Page 199 seql i st cor r ect ed. t xt t cc Ser ttt Phe aag Lys gat Asp tct Ser acc Thr at g Met gga Gy gac Asp 150 aac Asn aag Lys t ac Tyr t ac Tyr gt c Val 230 aat Asn gtc ttt Phe cgt Ar g cct Pr o tgg Tr p act Thr at t SIle tct Ser 120 ggc Gy at c SIle cgc Ar g aag Lys gag G u 200 ggc G y acc Thr t ac Tyr gat aat gac ctc gac Asp Asn Asp Leu Asp ggc acc ctc act gaa Gy Thr Leu Thr G u 45 gaa ctg ggt cgc gtc G u Leu G y Arg Val ttc ggg cgc gaa ggc Phe y Arg G u y 75 gga act tcg act gct G y Thr Ser Thr Ala gtc gcc gtc gaa ct a Val Ala Val G u Leu 105 gtt gtc cgt gga gag Val Val Arg Gy u 125 atc gag gtg ctc aac lie G u Val Leu Asn 140 cgc gta tac ttc ccg Arg Val Tyr Phe Pro 155 cgc atc atc cag tct Arg Ile Ile n Ser 170 cca ggt gga gtt ctg Pro G y G y Val Leu 185 tgg atc aat gag ct a Trp lie Asn G u Leu 205 tgg cca tgg gag gaa Trp Pro Trp G u G u 220 aag ttt gaa ggc aaa Lys Phe G u G y Lys 235 t ac Tyr 30 aac Asn ct c Leu gca Al a gcc Al a t ac Tyr 110 at c l Ie cgc Ar g gac Asp ggt Gy cac Hi s 190 gtt Val tgc Cys ggc G y cca cgt cta ggc agt Pro Arg Leu Gy Ser cag caa acc atg tgg Sn G n Thr Met Trp gaa gat gag ctg at t G u Asp G u Leu I e aaa acc atc gta gag Lys Thr Ile Val G u atg gct cca ctt gag IVet Ala Pro Leu G u 95 aag ccg ggc ttg gcc Lys Pro G y Leu Ala 115 gac aac gtg cgc atg Asp Asn Val Arg IVet 130 atg ttt gcc gat ggg IVet Phe Ala Asp y 145 cct tgg cca aag gcg Pro Trp Pro Lys Ala 160 ccg ctg aac ctg ttt Pro Leu Asn Leu Phe 175 gtt gct acc gac cac Val Ala Thr Asp His 195 gag gtc gaa cca ct g G u Val G u Pro Leu 210 cct cag ctg act gac Pro G n Leu Thr Asp 225 ttg gaa aaa gat cac Leu G u Lys Asp Hi s 240 gtc Val gat Asp gat Asp at c SIe gct Al a 100 aag Lys gtc Val t cc Ser cgc Ar g gca Al a 180 gct Al a ctt Leu cgt Ar g gtg Val ttg tgg Leu Trp 250 cag aag gtg caa G n Lys Val G n t aat gt ct ga t gt gcat gag <210> 150 <211> 255 <212> PRT <213> Corynebacter i um gl ut ai cum Page 200 seql i st cor r ect ed. t xt <400> 150 Met 1 Gy Leu Thr G u I le Pr o Gy Val Al a 145 Pr o Asn Thr G u Leu 225 Lys SIle Pr o Ser Tr p SIle G u G u Al a 115 Mt Gy Al a Phe Hi s 195 Leu Asp Hi s Asp 5 G n Thr G u Val Gy Asp Leu Ar g Leu Hi s 165 Lys Asp G u G n SIle 245 Asn Ser Arg Asp G n Leu y G u Leu Asp Ar g Tr p Al a Sy Asn Sy Asp 135 Sy Lys Leu Al a Lys 215 I le G u Asp Sy G u Phe Sy Val 105 Val SIle Ar g Ar g Pr o 185 Tr p Tr p Lys Leu Asp Leu Sy Ar g 75 Ser Val Ar g Val Tyr 155 I le Gy Asn Tr p G u 235 G n Asp G u Val Gy Al a Leu G u 125 Asn Pr o Ser Leu Leu 205 G u Lys Val Tyr Asn Leu Al a Al a Tyr 110 SIle Ar g Asp Gy Hi s 190 Val Cys Gy G n Pr o Pr o G n G u Lys let Lys Asp Met Pr o Pr o 175 Val G u Pr o Leu Asn 255 Al a Ar g G n Asp Thr Al a Pr o Asn Phe Tr p 160 Leu Al a Val G n G u 240 <210> 151 <211> 888 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(865) <223> FRXA02589 <400> 151 gcctaaattg cagcgagagg tctaaaaggt agtgctctag ggattcatcc aaactcacga atattgaagt tttaaagttg aacaggaaaa ataacaaata atg tct att tct gat 115 NIet Ser lie Ser Asp 1 Page 201 s aat tcc cgc gat caa tta gga gaa Asn Ser Arg Asp G n Leu Gy G u eql i st cor r ect ed.t ctg cca gct ggt Leu Pro Ala y t cc Ser ttt Phe aag Lys gat Asp tct Ser acc Thr at g Met gga Gy gac Asp 150 aac Asn aag Lys t ac Tyr t ac Tyr gt c Val 230 ttt Phe cgt Ar g cct Pr o tgg Tr p act Thr at t SIle tct Ser 120 ggc Gy at c SIle cgc Ar g aag Lys gag G u 200 ggc Gy acc Thr aat Asn acc Thr ct g Leu ggg Gy act Thr gcc Al a gt c Val gag G u gt a Val at c SIle 170 ggt Gy at c SIle cca Pr o ttt Phe tgg Tr p 250 gac Asp ct c Leu ggt Gy cgc Ar g 75 tcg Ser gt c Val cgt Ar g gt g Val tac Ty r 155 at c SIle gga Gy aat Asn tgg Tr p gaa 35u 235 ct c Leu act Thr cgc Ar g gaa G u act Thr gaa Gu gga Gy ct c Leu 140 ttc Phe cag G n gtt Val gag G u gag G u 220 ggc Gy gac Asp gaa G u 45 gt c Val ggc Gy gct Al a ct a Leu gag G u 125 aac Asn ccg Pr o tct Ser ct g Leu ct a Leu 205 gaa G u aaa Lys t ac Ty r aac Asn ct c Leu gca Al a gcc Al a t ac Tyr 110 at c SIle cgc Ar g gac Asp ggt Gy cac Hi s 190 gtt Val tgc Cys ggc Gy cca Pr o cag G n gaa G u aaa Lys at g Met 95 aag Lys gac Asp at g Met cct Pr o ccg Pr o 175 gtt Val gag G u cct Pr o ttg Leu cgt ct a Arg Leu caa acc G n Thr gat gag Asp u acc at c Thr Ile 80 gct cca Ala Pro ccg ggc Pro G y aac gt g Asn Val ttt gcc Phe Al a 145 tgg cca Trp Pro 160 ctg aac Leu Asn gct acc Al a Thr gtc gaa Val G u cag ctg G n Leu 225 gaa aaa G u Lys 240 xt cgg cct ct c Arg Pro Leu ggc agt gtc G y Ser Val atg tgg gat IVet Trp Asp ctg att gat Leu Ile Asp gt a gag at c Val Gu Ile ctt gag gct Leu G u Al a 100 ttg gcc aag Leu Ala Lys 115 cgc atg gt c Arg bet Val 130 gat ggg t cc Asp G y Ser aag gcg cgc Lys Al a Ar g ctg ttt gca Leu Phe Al a 180 gac cac gct Asp Hi s Al a 195 cca ctg ctt Pro Leu Leu 210 act gac cgt Thr Asp Ar g gat cac gt g Asp Hi s Val caa G n acg Thr gaa G u gtt Val ggc Gy gat Asp ttg Leu cgc Ar g ctg Leu cac Hi s 165 aag Lys gat Asp gag G u cag G n at c SIe 245 aat gag tac ttg Asn G u Tyr Leu gtc <210> 152 cag aag gtg caa G n Lys Val G n t aat gt ct ga t gt gcat gag Page 202 seqi i st car r ect ed. t xt <21 1> 255 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 152 N/t Ser I Ie Ser G31y Le u Thr (31u li1e Pr a G31y ValI A a 145 Pr a As n Thr (31u Le u 225 Ly s Pr a Ser Tr p I Ie (31u (31u A a 115 vb t G31y A a Ph e Hi s 1 95 Le u As p Hi s Asp Asn G3 n Ser Thr Phe a3 u Lys Val As p 70 G3 y Ser Asp Thr Leu l'b t Ar g G31y Leu Asp 150 Hi s As n 1 65 Lys Lys Asp Tyr a3 u Ty r G3 n Val 230 1 1 e As n 245 Ser As p Ar g Tr p A a a31y As n a31y As p 135 a31y Lys5 Le u A a Lys5 215 li1e a31u As p As p a31y a31u Ph e a31y ValI 1 05 ValI I Ie Ar g Ar g Pr a 1 85 Tr p Tr p Lys5 Le u Le u As p Le u a31y Ar g Ser ValI Ar g ValI Tyr 1 li1e a31y As n Tr p a31u 235 a31n G31u As p a31u ValI a31y A a Le u a31u 125 As n Pr a Ser Le u Le u 205 a31u Lys5 ValI Le u Tyr As n Le u A a A a Tyr 110 I Ie Ar g As p a31y Hi s 1 ValI cys a31y a31n Pr o Pr a a31n a31u vb t As p vb t Pr a Pr a 1 ValI a31u Pr a Le u As n 255 <210O> 153 <21 1> 948 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (925) <223> RXA00226 <400> 153 ccgcct gcgg t gt cagcgga gcgcgcccgg cgtct gaaaa ct gcact tgg t gagaacgt g at tgt tcat g at gt cacct g t tccat cggt acggaggggc at g aac t ta t cg at g 115 Page 203 seqi i st car r ect ed. t xt l'bt As n Leu Ser l'bt ccg Pr o at g vb t g ac As p cct Pr o ct g Le u gcc A a gt c ValI act Thr g at As p c aa O31n 1 50 gcc A a ct g Le u cca Pr o aag Ly s tt a Le u 230 c ag O31n tt c Ph e gcc A a ct c Le u ag a Ar g cct Pr o aaa Ly s at t I Ie g at As p 120 ct c Le u ct t Le u gt g ValI at g vb t ggt O31y 200 aaa Ly s cgt Ar g aag Ly s ac c Thr aat As n ccc Pr a gcc A a cct Pr a gcc A a ag c Ser gca A a g ac As p gct A a gcc A a 1 70 g at As p ag c Ser gcg A a gt t ValI ac c Thr 250 gt t ValI c ag O31n ag c Ser ggc G31y ct t Le u ggc G31y g at As p ggg G31y at c I Ie 140 cct Pr a tt g Le u cgg Ar g tt t Ph e gca A a 220 gt t ValI gcg A a ct g Le u ggc G31y ag g Ar g 45 aag Ly s ct c Le u ct t Le u gga G31y g aa (31u 125 ac c Thr ggt G31y gt t ValI at c I Ie ag g Ar g 205 gca A a g at As p at g vb t at c I Ie aaa Ly s gga G31y cgc Ar g g ac As p g at As p ggc G31y 110 act Thr aac As n gag O31u cgc Ar g gca A a 1 90 tt t Ph e cat Hi s cct Pr a t ct Ser g at As p gcc A a g ac As p ac a Thr t gg Tr p 80 t cg Ser aaa Ly s g aa (31u g aa (31u at c I Ie 1 60 t at Tyr ct t Le u g aa O31u gcg A a c ag O31n 240 gt g ValI tt c Ph e ct g Le u gt c ValI aat As n at c I Ie g aa O31u g aa O31u gct A a g ac As p 145 at c I Ie gca A a ac g Thr gag O31u ggg O31y 225 tt g Le u at c I Ie t ca Ser at t I Ie at c lIe ccg Pr a aac As n t gg Tr p gca A a at c I Ie 130 g at As p g ac As p gt g ValI ggc O31y gt t ValI 210 gcg A a cgg Ar g act Thr cgc Ar g tt c Ph e at c I Ie gca A a c aa O31n cca Pr a t gc cys 115 gt g ValI gcc A a cca Pr a cgt Ar g aat As n 1 ccg Pr a gt t ValI aaa Ly s cga Ar g ac c Thr cgc Ar g gcg A a c ag O31n cca Pr a ggt O31y 1 00 ct c Le u at c I Ie ac g Thr g ac As p gag O31u 1 cgg Ar g ct g Le u g aa O31u ag a Ar g at t I Ie 260 ct c Le u gcc A a g ac As p ct c Le u ct c Le u ct c Le u t ac Tyr aaa Ly s gga O31y ggt O31y 1 c ag O31n at t I Ie aag Ly s at t I Ie tt g Le u 245 ggc (31y agc cga ggg gt t gca t tg at t t gt ggt cct cgc gag cgc gcc Page 204 IN seql i st cor r ect ed. t xt SSer Arg Gy Val Ala Leu Ile Cys Gy Pro Arg u Arg Ala 265 270 275 t aaagccgat gcaaataaaa t t g 948 <210> 154 <211> 275 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 154 C1 IVet Asn Leu Ser IVet Pro Ala Phe Ala Thr Trp Val Leu I e Leu Asp 1 5 10 00 S Phe Ser Arg Thr Leu IVet Al a Ala His Asn Leu G n G y Lys Asn Al a 25 \O Leu Ile Phe Arg Ala Asp Ala Leu G n Pro Ala Ser Arg y Ala Asp 40 Cq Val Ile I le Ala Asp Pro Ala Arg Arg Al a Oy Oy Lys Arg lle Thr 55 Asn Pro Ala G n Leu Leu Pro Pro Leu Pro Ser Leu Leu Asp Ala Trp 70 75 Ile Asn G n Pro Leu Al a Val Lys Cys Ala Pro y Leu Asp Phe Ser 90 G u Trp Pro y Leu Val Ser Ile Ala Ser Val Asp y y Val Lys 100 105 110 G u Ala Cys Leu Tyr Thr Thr Asp Leu Ala Asp y G u Thr Arg G u 115 120 125 Ala Ile Val lie Lys Asp y Leu Ile Asp Arg lie Thr Asn Phe u 130 135 140 Asp Asp Ala Thr Gy n Asp Leu Ala Ala Ala Pro Gy u Phe Ile 145 150 155 160 IIe Asp Pro Asp G y Al a IIe Val Arg Ala G y Leu Val Arg His Tyr 165 170 175 Ala Val Arg G u G n Leu Trp IVet Leu Asp G u Arg Ile Ala Tyr Leu 180 185 190 Thr y Asn Arg lie Pro u y Thr Ser y Phe Arg Phe Ile u 195 200 205 G u Val Pro Leu Lys Lys Leu Lys Ser Ala IVet Ala Ala His Asp Ala 210 215 220 Sy Al a Val u lle Leu Val Arg y Val Asp Val Asp Pro Asp G n 225 230 235 240 Leu Arg Lys Arg Leu G n Leu Lys y Thr Lys Ala IVet Ser Val Val 245 250 255 Ile Thr Arg Ile y Ser Arg y Val Ala Leu Ile Cys y Pro Arg 260 265 270 G u Arg Ala 275 <210> 155 Page 205 seqI i st car r ect ed. t xt <21 1> 924 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (901) <223> RXN01885 <400> 155 gt ggcgt cgc agggat gt tc ct gcggcacc at tt t tgct g acacggat tt t tct aaggt t aat caagt aa ggt t tacct t aggt ggaact cacggat taa at c I Ie at c I Ie ac a Thr gt g ValI aag Ly s gcg Al a ac a Thr at c I Ie ct g Le u at t I Ie 1 50 gag O31u ccc Pr o g at As p cca Pr o gcc Al a c ag G n tt t Ph e ccg Pr o gt a ValI gcc Al a g aa 0Ou 120 cgc Ar g gag 0Ou at t I Ie ac g Thr at t I Ie 200 t ca Ser ct t Le u ct g Le u cca Pr o cgc Ar g gcg Al a g at As p at g vb t gca Al a t cg Ser aag Lys 1 70 ccg Pr o at t I Ie acc Thr gga G31y ct g Le u gga G31y t ac Tyr 75 at c I Ie act Thr t t g Leu gct Al a ct g Le u 155 gcg Al a gaa O31u cgc Ar g c ac Hi s aag Ly s gag G u ct t Le u at c I Ie tt a Le u 99c 0Gy ac c Thr cga Ar g 140 gt g ValI ct g Le u t gg Tr p c aa 0On t cc Ser aaa Ly s aac As n 45 99c G31y gga G31y cct Pr a ct t Le u at g vb t 125 at t I Ie cct Pr a gct Al a gct Al a gcc Al a 205 gca Al a gt g ValI ct t Le u gt g ValI gt g ValI gct Al a g aa O31u 110 c ag O31n ct g Le u g ac As p cgt Ar g gcg Al a 1 90 g ac As p g aa O31u 15 ct g Le u t ct Ser act Thr g at As p ggt G31y 95 ag c Ser ac c Thr aaa Ly s aat As n t cc Ser 1 75 tt g Le u at g vb t cgt Ar g cgc Ar g tt g Le u gca Al a ag c Ser 80 cct Pr a g ac As p g at As p cca Pr a gt c ValI 1 60 at c I Ie gcg Al a gcc Al a at g act I'b t Thr 1 gct ggt Al a 03 y cct gga Pro G31y acc ggt Thr G y cgt gac Ar g As p gac gcg As p Al a cac gag Hi s 03 u t cg tt t Ser Phe aag cac Lys Hi s 130 ggc ggg G31y (1y 145 t cc act Ser Thr aaa gt c Lys Val cgt gag Ar g O31u ct t ct a Leu Leu 210 acg aaa cct Thr Lys Pr o gga cat t gg G31y Hi s Tr p ggt cgt gaa G31y Ar g O31u gct acc gt g Al a Thr Val at c ct t ggc Il e Leu 03 y gat gca t gc Asp Al a Cys gt g cgc aat Val Ar g Asn 1 00 gat gt t gt c Asp Val Val 115 aag t tg gag Lys Leu 03 u ct g t ac ggc Leu Tyr 03 y gcg gt g aaa Al a Val Lys 1 aat gcc cgc Asn Al a Ar g 1 gca ggg ttc Al a 03 y Phe 1 t cc ct c aag Ser Leu Lys Page 206 cgg Ar g aac As n 230 aag Ly s tt g Le u ct g Le u at t I Ie t tc Ph e aag Ly s g at As p c aa (31n gag (31u 250 gcc A a gaa (31u ccg Pr o 235 cat Hi s caa O31n seqI i st car r ect ed. t xt ggg ct a aaa ggt gt c t tc acg at t gt g agg G31y Leu Lys G31y Val Phe Thr I Ie Val Ar g 220 225 gat ctg cgc aag cga gtg ctc gga atg cga Asp Leu Arg Lys Ar g Val Leu 03 y N/t Ar g 240 245 aaa gat cac t ta ggt gcg gt t ggc at c at t Lys As p Hi s Leu G31y Ala Val 31y Ilie Ilie 255 260 t agggat ct g aaat ggaggg gt g <210O> 156 <21 1> 267 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn <400> 156 I'bt Thr Thr A a Pr o Thr Ar g As p Hi s Ser Ly s G31y 145 Ser Ly s Ar g Le u Ph e 225 G31y G31y A a I Ie As p ValI As p 115 Ly s Le u A a As n A a 1 95 Ser I Ie Lys Pro I Ie Hi s Tr p I Ie Ar g O31u Th r Thr Val Val Leu 03 y Lys 70 A a Cys A a Arg Asn Thr 1 00 Val Val Ilie Leu 03 u Leu Tyr 03 y Il e 150 Val Lys O31u 1 65 Ala Ar g Pr o 1 80 03 y Phe Asp Leu Lys Arg Val Ar g Asn I Ie Le u Thr (31u G31y As n As n G31y vb t 135 Hi s As p I Ie ValI As n 215 ValI Pr o A a O31n 40 Ph e Pr o ValI A a O31u 120 Ar g Ou le Thr I Ie 200 Le u I Ie O31u Ar g 25 Ph e A a A a Ar g Thr 1 05 A a O31u Le u A a ValI 1 85 As n Ly s Ser Ser 10 Le u Le u Pr o Ar g A a 90 As p vb t A a Ser Ly s 1 70 Pr a I Ie As p Thr G31y Le u G31y Tyr li1e Thr Le u A a Le u 1 A a O31u Ar g O31u Ser Ly s As n G31y G31y Pr a Le u vb t 125 li1e Pr a A a A a A a 205 Le u A a ValI Le u ValI ValI A a O31u 110 O31n Le u As p Ar g A a 1 As p Ly s (31u Le u Ser Thr As p G31y Ser Thr Ly s As n Ser 1 Le u vb t G31y O31n Pro Asp Leu Ar g Lys 235 Page 207 seqI i st car r ect ed. t xt Val Leu G31y I'bt Arg Lys Thr Phe Thr Ou His Lys Asp His Leu G1y 245 250 255 AlaVal y Ilie Ilie Leu (1n Lys Ar g Aa (1n 260 265 <210O> 157 <21 1> 924 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (901) <223> FRXA01885 <400> 157 gt ggcgt cgc agggat gt tc ct gcggcacc at tt t tgct g acacggat tt t tct aaggt t aat caagt aa ggt t tacct t aggt ggaact cacggat taa at g act acg aaa cct 11! I'bt Thr Thr Lys Pro at c I Ie at c I Ie ac a Th r gt g ValI aag Ly s gcg Al a ac a Thr at c I Ie ct g Le u at t I Ie 1 50 cca Pr o gcc Al a c ag O31n tt t Ph e ccg Pr o gt a ValI gcc Al a g aa G31u 120 cgc Ar g gag (31u t ca Ser ct t Le u ct g Le u cca Pr o cgc Ar g gcg Al a g at As p at g vb t gca Al a t cg Ser aag Ly s 1 70 acc Thr gga G31y ct g Le u gga G31y t ac Tyr 75 at c I Ie act Thr t t g Le u gct Al a ct g Le u 155 c ac Hi s aag Ly s gag G31u ct t Le u at c I Ie tt a Le u ggc G31y ac c Thr cga Ar g 140 gt g ValI t cc Ser aaa Ly s aac As n 45 ggc G31y gga G31y cct Pr o ct t Le u at g vb t 125 at t I Ie cct Pr o gca Al a gt g ValI ct t Le u gt g ValI gt g ValI gct Al a g aa O31u 110 c ag O31n ct g Le u g ac As p g aa G u 15 ct g Le u t ct Ser act Thr g at As p ggt 0Gy 95 ag c Ser ac c Thr aaa Ly s aat As n t cc Ser 1 75 cgt Ar g cgc Ar g tt g Le u gca Al a ag c Ser cct Pr a g ac As p g at As p cca Pr a gt c ValI 1 at c I Ie 1 gct Al a cct Pr a ac c Thr cgt Ar g g ac As p c ac Hi s t cg Ser aag Ly s ggc G31y 145 t cc Ser aaa Ly s ggt G y gga 0Gy ggt 0Gy g ac As p gcg Al a gag 0Ou tt t Ph e c ac Hi s 130 ggg 0Gy act Thr gt c ValI gga G y ggt 0Gy gct Al a at c I Ie g at As p gt g ValI g at As p 115 aag Ly s ct g Le u gcg Al a aat As n cat Hi s cgt Ar g ac c Thr ct t Le u gca Al a cgc Ar g 1 00 gt t ValI tt g Le u t ac Tyr gt g ValI gcc Al a 1 gag gat at t gct 0 u Asp I e Al a gcg ct g gct cgt Ala Leu Ala Arg Page 208 IN seql i st cor r ect ed. t xt ccc atc acg gtg ccg gaa tgg gct gcg ttg gcg cgt gag gca ggg ttc 691 Pro Ile Thr Val Pro G u Trp Ala Ala Leu Ala Arg G u Ala y Phe 185 190 195 C gat gtg att aat att cgc caa gcc gac atg gcc ctt cta tcc ctc aag 739 Asp Val Ile Asn Ile Arg G n Ala Asp Iet Ala Leu Leu Ser Leu Lys S200 205 210 Scgg aac ctg aag gat gaa ggg cta aaa ggt gtc ttc acg att gtg agg 787 Arg Asn Leu Lys Asp G u y Leu Lys y Val Phe Thr Ile Val Arg 215 220 225 CK1 aac gtg att agc caa ccg gat ctg cgc aag cga gtg ctc gga atg cga 835 0 Asn Val lie Ser G n Pro Asp Leu Arg Lys Arg Val Leu Gy IVet Arg 00 230 235 240 245 0 0 aag act ttc acc gag cat aaa gat cac tta ggt gcg gtt ggc atc att 883 C Lys Thr Phe Thr G u His Lys Asp His Leu G y Ala Val G y IIe IIe 250 255 260 ttg cag aag aga gcc caa tagggatctg aaatggaggg gtg 924 Cq Leu G n Lys Arg Ala G n 265 <210> 158 <211> 267 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 158 IVet Thr Thr Lys Pro Ile Ile Pro u Ser Thr His Ser Ala u Arg 1 5 10 Ala G y G y His Trp IIe Leu Ala Arg Leu G y Lys Lys Val Leu Arg 25 Pro Gy y Arg G u Thr Thr G n Phe Leu Leu G u Asn Leu Ser Leu 40 Thr y Ala Thr Val Val G u Phe Ala Pro y Leu y Val Thr Ala 55 Arg Asp Ile Leu y Lys y Pro Ala Arg Tyr I e y Val Asp Ser 70 75 Asp Ala Asp Ala Cys Al a Asn Val Arg Ala lle Leu Pro Ala y Pro 90 His G u Val Arg Asn Thr Asn Ala Thr Asp Thr G y Leu G u Ser Asp 100 105 110 Ser Phe Asp Val Val I e Gy G u Ala IVet Leu Thr IVet G n Thr Asp 115 120 125 Lys His Lys Leu G u Leu IVet Arg G u Ala Ala Arg lie Leu Lys Pro 130 135 140 G y G y Leu Tyr G y IIe His G u Leu Ser Leu Val Pro Asp Asn Val 145 150 155 160 Ser Thr Ala Val Lys G u Asp lie Ala Lys Ala Leu Ala Arg Ser lie 165 170 175 Lys Val Asn Ala Arg Pro lie Thr Val Pro G u Trp Ala Ala Leu Ala 180 185 190 Arg u Al a y Phe Asp Val Ile Asn Ile Arg n Ala Asp Iet Ala Page 209 seqI i st car r ect ed. t xt 195 200 205 Leu Leu Ser Leu Lys Arg Asn Leu Lys Asp G31u G31y Leu Lys G31y Val 210 215 220 Phe Thr Ilie Val Ar g Asn Val Ilie Ser (31n Pr o Asp Leu Arg Lys Arg 225 230 235 240 Val Leu G31y I'b t Ar g Lys Thr Phe Thr G31u Hi s Lys Asp His Leu G31y 245 250 255 Ala Val 31y Ilie Ilie Leu (1n Lys Ar g Aa (1n 260 265 <210O> 159 <21 1> 894 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (871) <223> RXA02592 <400> 159 aaacaccacc at tgt t tcct ccat cgacga t gccat cacc cggcgaagac at ccgcgacc t caact ggac ccgcgcat aa accatt t tgc gat ggat gaa at g gcc t ca t tt ccg 11! I'bt Ala Ser Phe Pr o gag G31u tt a Le u gga G31y g at As p ggc G31y t ac Tyr g ac As p ccc Pr o t cc Ser ccg Pr o t ct Ser ct g Le u t ct Ser ccc Pr o t cc Ser c ac Hi s t cc Ser 120 ccg Pr o ct t Le u tt c Ph e ct c Le u gcc A a t ac Tyr g aa G31u t ca Ser g ac As p g aa (31u cgt Ar g aaa Lys5 g at As p gga G31y t t c Ph e 75 ccc Pr a gt c ValI gt g ValI t t g Le u cgc Ar g t ac Tyr ac c Thr tt a Le u gcc A a g ac As p cgc Ar g gt g ValI gga G31y 140 tt g Le u g aa G31u t cg Ser 45 aat As n g aa G31u gt t ValI gga G31y t ac Tyr 125 g aa (31u gct A a c aa (31n agt Ser g ac As p gcc A a ggc G31y t cc Ser 110 t cc Ser g aa (31u ac c Thr 15 ac c Thr ct g Le u ct g Le u tt t Ph e g aa G31u 95 ggc G31y t cc Ser at g vb t tt g Le u cga Ar g gt g ValI aaa Lys5 gag G31u at g vb t ct c Le u aac As n ct c Le u 1 ggc G31y cct Pr a gcg A a gt t ValI ac a Thr t cc Ser g ac As p gt t ValI cgc Ar g 145 ag g Ar g g ac As p gct A a ct c Le u ct g Le u gca A a ct g Le u gca A a 130 gt c ValI t cg Ser at c lIe tt g Le u g ac As p ggc G31y gct A a ccg Pr a 115 g aa G31u ac c Thr t gg Tr p tt t Ph e t ct Ser gt c ValI gcc A a ggc G31y 1 00 tt t Ph e cat Hi s cgc Ar g Page 210 ggc ggc ct g gca G3 y G3 y Leu Al a 1 50 ggc cat gaa acc a3 y Hi s a3 u Thr cgc gat cgc t ac Ar g Asp Ar g Tyr 1 85 ggg gag t ca ct g a31y a31u Ser Leu 200 gcc t cc gt g ggc Al a Ser Val a31y 215 ccg t at t gg gt c Pr o Tyr Tr p Val 230 gcg gt a agt aac Al a Val Ser Asn aggaat tcat cgc <210> 160 <211> 257 <212> PRT <213> Cor ynebact <400> 160 l'b t Al a Ser Phe a31y Ar g Se r Tr p Pro Asp I Ie Phe Al a Al a Leu Ser Val Leu Asp Val Thr Leu a31y Al a Ser Al a Al a a3 y 1 00 Asp Leu Pro Phe 115 Val Ala a31u Hi s 130 Ar g Val Thr Ar g 145 at c I Ie gga G31y 1 70 ac g Thr tt t Ph e aat As n t gg Tr p tt g Le u 250 ag c Ser t gg Tr p aaa Lys5 gt g ValI g aa a31u 220 at g vb t tt g Le u seql i st car r ect ed. t xt tac acc at t t gg t ta ggg ccc tt c Ty r Thr I Ie Tr p Leu a31y Pr o Phe 1 gaa cac t ac gt t ggc gga gaa tt t G31u Hi s Ty r Val G31y 31y G31u Phe 175 180 cac ggg cac ccg cct aag aac gtt His a31y Hi s Pr o Pr o Lys Asn Val 190 195 t cc t gc cgg gag ggg ct g gaa t gg Ser Cys Ar g a31u a31y Leu a31u Tr p 205 210 t tg gt t gcc gct t tt ccc cgc t ac Leu Val Al a Al a Phe Pr o Ar g Tyr 225 gt t aaa gt c cca gt g ct c cga gaa Val Lys Val Pr o Val Leu Ar g a31u 240 gt g t tt aaa aag cac t gaggtt tt g Val Phe Lys Lys Hi s 255 er i urn gl ut anm cumn Pr a (31y Tyr (31u (31y Thr li1e Le u ValI Ser Le u Le u As n I Ie (31y Ph e ValI As p Al a 135 (31y Al a As p 25 Al a (31y (31y ValI (31y 1 05 Ph e Tr p Al a Ar g Lys5 As p (31y Ph e Pr a ValI ValI Le u Le u 1 Le u (31u Ser As n (31u ValI (31y Tyr 125 (31u Tyr Al a a31n Ser As p Al a (31y Ser 110 Ser (31u Thr Th r Th r Le u Le u Ph e (31u (31y Ser vb t I Ie Leu Oy Pro Phe a1y ay His au Thr ay Leu Trp au His Tyr Val Page 211 G y G y G u Phe 180 Pro Lys Asn Val 195 Sy Leu G u Trp 210 Phe Pro Arg Tyr 225 Val Leu Arg G u Hi s 165 Al a Phe Gy Hi s Phe 245 seql i st cor r ect ed. t xt 170 Arg Asp Arg Tyr Thr Lys Lys His 185 G y u Ser Leu Phe Asn Val Ser 200 205 Al a Ser Val Gy Asn Ala G u Leu 215 220 Pro Tyr Trp Val Trp Trp IVet Val 230 235 Al a Val Ser Asn Leu Val Leu Val 250 175 Hi s Ar g Al a Val Lys 255 Pr o G u Al a Pr o 240 Lys <210> 161 <211> 720 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(697) <223> RXN01795 <400> 161 agaccatatt gaagacctcg aagctgt t ga gcctggctac caacttcgct gaatacggtg tcccacaatt ccgcgaacgt at t SIle ggc Gy ggc Gy cgc Ar g gcg Al a gtt Val ggt Gy ttg Leu cgt Ar g cgc Ar g aag Lys gtt Val ccc Pr o cgt Ar g ggc Gy aac Asn acc Thr gac Asp gt c Val gt g Val gat Asp ttg Leu ggt Gy gag Gu ggc Gy at g Met ccc Pr o ctt Leu gac Asp 75 cac Hi s aca Thr cgg Ar g ttt Phe ccg Pr o aca Thr aag Lys ccc Pr o cgt Ar g tgg Tr p gct Al a gat Asp t at Tyr aac Asn 45 cgc Ar g t ac Tyr gat Asp gga Gy aga Ar g ttc Phe aag Lys aac Asn at c SIle t ac Tyr gag G u t ac Tyr 110 at t SIle aag Lys 15 act Thr aac Asn cct Pr o gt c Val cca Pr o 95 cat Hi s cac Hi s gcc Al a cac Hi s gag G u aag Lys 80 t cc Ser t at Tyr at cgt caagc ct cgcct gt a gtg ctc att gtt ggc 11! Val Leu Ile Val Gy 1 cca gct cct acc cat 16; Pro Ala Pro Thr His ggc gaa gcg ctc aaa 21 G y G u Al a Leu Lys atg aag atc atg cct IVet Lys Ile IVet Pro ggc gaa aac ttc acc G y G u Asn Phe Thr ggc atg att agt cac G y Met IIe Ser His aaa acc ctt atc gcc Lys Thr Leu lie Ala 100 gaa aaa aat cga gca G u Lys Asn Arg Ala 115 ttc ccc gat gac ttt 49! Phe Pro Asp Asp Phe caa t cg G n Ser Page 212 gag O31u gt t ValI 1 50 t ac Tyr ct g Le u gct Al a seqI i st car r ect ed. t xt 120 125 130 ttg gga tca aac acc gaa gtc cgc cgc caa atc Leu G31y Se r Asn Thr O31u Val Ar g Arg O31n Ilie 140 145 cct gt a ggt at g cac gct gt g ggt gag cga ct g Pr o Val 31y Nib t Hi s Al a Val G31y O31u Ar g Leu 155 160 ggg aat t ac act ccc gt c gat ct a gag gaa cag G31y Asn Tyr Thr Pr o Val Asp Leu O31u O31u O31n 170 175 acg ct c t cc at t aag gaa cgt ct c gcg ct g gct Thr Leu Ser Ilie Lys O31u Ar g Leu Al a Leu Al a 185 190 t aagt agat a t at gaagccc acc aat gct As n Al a aac ct g Asn Leu 1 gcg t ac Al a Ty r 1 cag gaa O31n (1u <210O> 162 <211> 199 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 162 Val Leu I Ie Val Pr o G31y G31y G31y Ly s O31u Ph e O31n 145 Ar g (31u Pr o Al a li1e As n I Ie Le u As n 115 As p G31y vb t Hi s G31y le Hi s 03 y Lys 03 y Pr o Ar g Thr Al a 70 Hi s Val Al a 03 y Al a Leu Phe 03 u Al a Val 150 Leu Tyr 1 65 Ty r Leu Ar g Pr o ValI Thr li1e Ty r G31y Th r Ph e 135 Pr o Th r O n As p O31y As p O31u Ly s Ar g O31y 1 Ar g O31y ValI As n Le u 1 O31y vb t Pr a Le u As p Hi s Thr Ar g As n vb t 1 Thr I Ie As p Tyr As n Ar g Tyr As p O31y Ar g 125 O31u Al a ValI a u Ly s Thr As n Pr a ValI Pr a Hi s O31n Ar g O31y Le u 1 Le u Leu Al a Asp 1 03 n 03 u Al a As p Page 213 seql i st cor r ect ed. t xt <210> <211> <212> <213> <220> <221> <222> <223> 163 535 DNA Cor ynebact eri um gl ut am cum CDS (535) FRXA01795 <400> 163 agaccatatt gaagacctcg aagctgttga gcctggctac caacttcgct gaatacggtg tcccacaatt ccgcgaacgt at cgt caagc ct cgcct gt a gt g Val 1 ctc att gtt Leu II e Val att cgc cgt gac lie Arg Arg Asp acc Thr ggc ttt gat ttc G y Phe Asp Phe cac cca gct cct His Pro Ala Pro acc cat Thr His ggc cct cgc G y Pro Arg gac atg ccg tat Asp IVet Pro Tyr aag Lys act gcc ggc gaa Thr Ala G y G u gcg ctc aaa Ala Leu Lys atc atg cct Ilie Mat Pro ggc gtg aag gat gtc ccc aca aac aac aac cac atg aag Sy Val Lys Asp Val Pro Thr Asn Asn Asn His Vet Lys 45 cgc acc Arg Thr gtt gaa gtg ctt Val G u Val Leu aag Lys cgc atc cct gag Arg lie Pro u gaa aac ttc acc G u Asn Phe Thr gcg Al a atc ccc aaa gat lie Pro Lys Asp ccc tac tac gt c Pro Tyr Tyr Val aag Lys ggc atg att agt Sy IVet Ile Ser gtt tac cgt cgc Val Tyr Arg Arg ttg Leu cac cgt gat gag His Arg Asp G u cca Pr o 95 t cc aaa acc ctt Ser Lys Thr Leu atc gcc I e Al a 100 ggt ggc ggc ggg ggt aca tgg gga tac cat tat gaa aaa aat cga gca Sy y y y y Thr Trp y Tyr His Tyr u Lys Asn Arg Ala 105 110 115 ttg acc aac Leu Thr Asn 120 cgc gag cgg gct Arg G u Arg Ala aga Ar g 125 att caa tcg ttc lie n Ser Phe ccc Pr o 130 gat gac ttt Asp Asp Phe gag ttt ttg gga tca aac G u Phe Leu G y Ser Asn 135 <210> 164 <211> 145 <212> PRT acc Thr 140 caa gtc cgc cgc G n Val Arg Arg <213> Corynebacter i um gl ut ai cum <400> 164 Val Leu Ile Val Gy Ile Arg Arg Asp 1 5 Pro Ala Pro Thr His G y Pro Arg G y Thr G y Phe Asp Phe Lys Hi s 10 Asp IVet Pro Tyr Thr Al a Sy G u Ala Leu Lys G y Val Lys Asp Val Pro Thr Asn Asn Asn His Page 214 seqI i st car r ec 40 I'bt Lys I Ie I'bt Pro Ar g Thr Val O31u Val Leu 55 G31y (31u As n Phe Thr Ala Ilie Pro Lys Asp Asp 70 G31y I'b t I Ie Ser Hi s Val Ty r Ar g Ar g Leu Hi s 90 Lys Thr Leu I Ie Al a G31y 31y G31y 31y G31y Th r 100 105 O31u Lys As n Ar g Al a Leu Thr As n Ar g O31u Ar g 115 120 Phe Pr o As p As p Phe O31u Phe Leu G31y Se r As n 130 135 O31n 145 <210O> 165 <21 1> 1614 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1591) <223> RXA01214 <400> 165 gt tgat at ga tgtggggt gt t tat gaaaat t tgt t tgagg gccagagt tg aat cgt cgga ctt tt t tcaa aggggccggg ted. t xt Lys Ar g Pr o Ty r Ar g As p Trp G31y Al a Ar g 125 Thr 03 n 140 Pr o ValI Pr o Hi s O31n Ar g gagt gaaggc gcat gt tgtt gt g ValI ggt G31y ggt G31y ggg G31y aat As n gt c ValI t gg Tr p ggt G31y ggg G31y cgt Ar g agt Ser act Thr gt t ValI ggc (31y c ag O31n gag (31u gga G31y at t I Ie tt g Le u gt g ValI aag Lys5 gt g ValI ccg Pr o t ct Ser t t g Le u ggg G31y 75 at a I Ie t t g Le u ct g Le u cgg Ar g ag c Ser ccg Pr o ccg Pr a aac As n ccg Pr a gcg Al a tt g Le u c ac Hi s gt c ValI tt g Le u tt g Le u at t I Ie 110 t cc Ser at t I Ie gcc Al a ac g Thr gt g ValI g aa (31u g at As p gt g ValI 1 t ca Ser cca Pr a ct g Le u aag Lys5 aag Lys5 at g vb t ggt (31y ct g Le u g at As p cca Pr a gag (31u ac g Thr aaa Lys5 ac c Thr ggt G31y gca Al a g at As p gca Al a gct Al a t gg Tr p ggt G31y act Thr ccg Pr a 115 gca Al a gt g ValI g at As p c ag O31n ggt O31y g at As p gt g ValI 1 00 c ac Hi s Page 215 seql i st cor r ect ed. t xt ccg Pr o gat Asp ttg Leu 150 gca Al a ccg Pr o gag G u aat Asn ttc Phe 230 t ca Ser gat Asp tgg Tr p tct Ser ccc Pr o 310 cct Pr o aaa Lys at g Met gac Asp ggg Gy 120 gcc Al a gcg Al a gac Asp gtt Val gac Asp 200 at t SIle gt g Val acg Thr cct Pr o at c SIle 280 ggt Sy ttc Phe gat Asp acc Thr acc Thr 360 gt g Val ggg Gy ttg Leu cgt Ar g ct g Leu 170 at g Met ccc Pr o aat Asn aac Asn acc Thr 250 gac Asp gt g Val gag G u at g Met gct Al a 330 cct Pr o t cc Ser gac Asp cag G n tgg Tr p ggt G y 155 gat Asp gat Asp gat Asp gcg Al a ggc G y 235 tt c Phe cgc Ar g gag G u gac Asp t ca Ser 315 gcg Al a gac Asp at c SIle cat Hi s acg Thr t ac Tyr 140 ttg Leu ct g Leu cac Hi s ctt Leu cac Hi s 220 t cc Ser caa G n acc Thr ct a Leu aac Asn 300 gat Asp caa G n gt c Val aac Asn gac Asp 380 tgg tca Trp Ser 125 cac ccg His Pr o gcg ggg Al a G y cca cgc Pro Arg cgc ttc Arg Phe 190 ggg ct g G y Leu 205 ttt gat Phe Asp aat at g Asn IVet gtc att Val lile acc ttg Thr Leu 270 gag ccc G u Pro 285 tac ggc Tyr G y tcc ttc Ser Phe gca cct Ala Pro at c gat Ile Asp 350 gat ct a Asp Leu 365 cag cca G n Pro cca acg tgg act gtg Pro Thr Trp Thr Val 130 cac act cat ggc ct g His Thr His Gy Leu 145 atg atc att gtg gaa IVet Ile Ile Val u 160 gag tac ggt gtg gac G u Tyr Gy Val Asp 175 tta gaa gac ggt tcc Leu G u Asp G y Ser 195 ttg ggc gat acc ccc Leu G y Asp Thr Pr o 210 gcc acc acg cgc cgg Ala Thr Thr Arg Arg 225 cgg ttc tat aac ttg Arg Phe Tyr Asn Leu 240 gcc agc gat tcc ggt Al a Ser Asp Ser G y 255 gct att ggc cca ggc Ala lie y Pro y 275 ggc gag gac gtc acc Gy G u Asp Val Thr 290 gtc cct gat gat gag Val Pro Asp Asp G u 305 cag ctg ctc acc at c G n Leu Leu Thr Ile 320 gct ttg ccg ggc gtg Ala Leu Pro y Val 335 gcc act gaa cgc acc Ala Thr G u Arg Thr 355 cag atg gac atg cag Sn bet Asp Ie t n 370 gcc Al a aca Thr gat Asp gat Asp 180 ctt Leu act Thr gtt Val gcg Al a ttg Leu 260 gag G u ttg Leu ttc Phe acc Thr ct g Leu 340 ttc Phe cgc Ar g aat Asn ggt G y gaa G u 165 at t SIe gat Asp gcc Al a cgg Ar g ttt Phe 245 ct g Leu cgg Ar g gaa G u gt g Val ggc G y 325 gt g Val at c SIe gtt Val 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 gaa gt g t gg G u Val Trp 385 Page 216 att gtc acc aac lie Val Thr Asn gac aac tcc gac tgg ccc cac Asp Asn Ser Asp Trp Pro His 390 395 aag gtg ctg aaa ttt gaa ggc Lys Val Leu Lys Phe G u y 410 tgg aaa gac acc gtc ggc ctg Trp Lys Asp Thr Val G y Leu 425 gtg gaa ttt ggc cac tac cca Val G u Phe Gy His Tyr Pro 440 tgc cac atg ctc tac cac gag Cys Hi s IVet Leu Tyr Hi s u 455 460 atc gtg gag cca ggc gac gag lie Val Gu Pro Gy Asp u 470 475 ggc tcc agc att gac tcc gcc Sy Ser Ser lie Asp Ser Al a 490 gggcggcgtc gat <210> 166 <211> 497 <212> PRT <213> Cor ynebact er i um gl ut ar seql i st cor r ect ed. t xt aac ttc cat gtc cac gac gcc cgg Asn Phe His Val His Asp Ala Arg 400 acc gac gta gag ctc ttc aac gac Thr Asp Val G u Leu Phe Asn Asp 415 420 cca ccg gga gca acc gca act tta Pro Pro Gy Ala Thr Ala Thr Leu 430 435 gac ccg caa tgg ccc tac atg tat Asp Pro G n Trp Pro Tyr IVet Tyr 445 450 gat caa ggc atg atg ggg cag ttc Asp Gn y et vbt y G n Phe 465 ccg gcg gcg gtg ctg ggg tcg ggc Pro Ala Ala Val Leu Gy Ser Gy 480 ggc gga cat gcg cac taggggcgtg G y G y Hi s Al a Hi s 495 1315 1363 1411 1459 1507 1555 1601 1614 <400> 166 Val Leu Al a Al a Thr Val Val Gy Ala G n Val Leu Val Ala 1 Ser Pr o Leu Lys Lys Met Gy Tr p Hi s 145 Asp Al a Al a Tr p Gy Thr Pr o 115 Val Leu 5 Ar g Leu Thr Phe Asp Hi s Ser Asn Gy Tyr Thr G u Gy Hi s Hi s SIle Al a 135 Hi s Gy Ar g 40 Ser Thr Val Gy Gy 120 Al a Al a Gy G u G n Hi s Asp Met 105 Pr o Thr Tyr 10 G u Pro Arg G y Ser Ser I e Leu Pr o Leu G y Pro 75 Val Ile Asn 90 Lys Leu Pro G y G n Thr Leu Trp Tyr 140 Arg G y Leu 155 Page 217 Thr Val Asp Thr Asn Al a Tr p 125 Hi s Al a Cys Ser Pro Ile Phe Al a Thr Thr Val Val Asp u Al a Asp Pro Thr Hi s Thr 'e t Ile 160 seqI i st car r ect ed. t xt I Ie Val O31u Asp O31u Al a Thr Asp Lys Leu Asp Leu Pro Ar g O31u Tyr 1 1 65 G31y As p As p Thr 225 Tyr As p G31y As p As p 305 Le u Pr o O31u As p Tr p 385 Hi s Le u Thr Pr o vb t 465 As p Ser 1 95 Pr o Ar g Le u G31y G31y 275 Thr Ou le ValI Thr 355 O31n ValI Al a As n Thr 435 vb t O31n I Ie As p Al a Ar g Ph e 245 Le u Ar g O31u ValI O31y 325 ValI I Ie ValI As n Ph e 405 O31y Al a Hi s ValI Le u O31u O31y 215 Ar g As p O31u O31u ValI 295 As p Ser Ser As n ValI 375 As n ValI Ly s O31u Hi s 455 ValI Le u 1 85 Le u Thr Le u Ar g O31n 265 ValI Ph e G31y As p O31u 345 Ph e I Ie As p Ly s Thr 425 G31y Le u Pr o 1 70 vb t Pr a As n As n Thr 250 As p ValI O31u vb t Al a 330 Pr a Ser As p Tr p Ph e 410 ValI Hi s Tyr G31y As p As p Al a G31y 235 Ph e Ar g O31u As p Ser 315 Al a As p I Ie Hi s Pr a 395 O31u G31y Tyr Hi s As p 475 Ar g G31y 205 Ph e As n ValI Thr O31u 285 Tyr Ser Al a I Ie As p 365 O31n As n Thr Pr a As p 445 As p Pr a Ph e 1 Le u As p vb t I Ie Le u 270 Pr a O31y Ph e Pr a As p 350 Le u Pr a Ph e As p Pr a 430 Pr a O31n Al a Le u Le u Al a Ar g Al a 255 Al a O31y ValI O31n Al a 335 Al a O31n O31u Hi s ValI 415 O31y O31n O31y Al a Leu Oy Ser Oy Thr Oy Ser Ser Ilie 485 Ser Al a 0 y 0 y Hi s Al a <210O> 167 Page 218 <211> <212> <213> <220> <221> <222> <223> seql i st cor r ect ed. t xt 588 DNA Cor ynebact eri um gl ut am cum CDS (588) RXA01250 <400> 167 ctg gat atc ggc ggc Leu Asp lie G y G y 1 5 atc gaa gcc aag i e G u Ala Lys tgg gga tac gtc Trp y Tyr Val tct gac Ser Asp acc ggg gat Thr G y Asp gat at c acc Asp II e Thr gcc att gag gcc Ala l e G u Al a gcc ggc gac gtc Al a y Asp Val ct c cag gtc Leu G n Val tgg cat ggc Trp His G y aat gac ctg cct Asn Asp Leu Pro agc acc tcc atc Ser Thr Ser Ile ggt gtg ccc ggc G y Val Pro G y cac Hi s atc gca I e Al a ctc cac aac gca Leu His Asn Al a gcc gac Al a Asp 55 atg acc cag gac IVet Thr G n Asp ccc Pr o att gaa cct ggc lie u Pro y tct ttc tcc tat Ser Phe Ser Tyr gtt Val ttt gaa gtc ccc Phe G u Val Pro ggt ggc acc tac Sy y Thr Tyr ttc tac cat tcc cac acc ggc ctg cag ctt gat cgc Phe Tyr His Ser His Thr G y Leu G n Leu Asp Arg 90 ggc ctc cac G y Leu Hi s cag gac gtc G n Asp Val 115 cca ctg atc at c Pro Leu Ile Ile cgt Ar g 105 gac ccg caa gac Asp Pro G n Asp gct gag gac Al a u Asp 110 gat ggc att Asp G y I e gag tgg acc atc Su Trp Thr lle ctc gac gac tgg Leu Asp Asp Trp gt c Val 125 cag ggc G n G y 130 act ccc gac gat Thr Pro Asp Asp gag 35u 135 ctc gac aag ctc Leu Asp Lys Leu gga atg ggt tcg Sy et y Ser ggc Gy 145 gac cat aac ggg Asp Hi s Asn G y atg gga atg gga e t Gy e t Gy ggt Gy 155 cac ggc cag atg His Gy G n Met cac ggc acc ccg His y Thr Pro gac Asp 165 cgg gta ctg ggc Arg Val Leu y gat gtc ggc gat Asp Val y Asp gt g at g Val Met 175 tat ccg cac tac ctc atc aac gga cgt atc ccc cgt gct cac cgg acc Tyr Pro His Tyr Leu I e Asn y Arg lie Pro Arg Ala His Arg Thr 180 185 190 ttc gag gct cgc Phe G u Ala Arg 195 <210> 168 <211> 196 <212> PRT <213> Corynebacteri um gl utam cum Page 219 seqI i st car r ect ed. t xt <400> 168 Leu Asp I I e 03 y Thr As p Pr o G31y G31y O31n G n O31y 145 Hi s Tyr Ph e As p Thr Le u O31u Thr Hi s ValI 115 Thr Hi s Thr Hi s A a 1 G31y A a As p As n G31y Ph e Pr o Tr p As p G31y As p 1 65 Le u HIe 0u Al a Lys Thr Trp Gy Tyr Val Ser Asp O31u Pr o A a Ser Hi s I Ie I Ie G u 135 vb t ValI As n A a O31u 40 As p Ph e Ser I Ie ValI 120 Le u G31y Le u G31y Thr Ser G31y Ser Hi s Ar g 1 05 Le u As p vb t G31y Ar g 1 85 A a Thr ValI Tyr Thr 90 As p As p Ly s G31y G31y 1 70 li1e G31y Ser Pr o ValI G31y Pr a As p Le u G31y 1 As p Pr a ValI Hi s vb t O31u O31n As p ValI 125 G y G31y G31y A a Le u Tr p Thr ValI Le u A a 110 As p vb t O31n As p Hi s 1 O31n Hi s O31n Pr a As p O31u O31y G y vb t ValI 1 Ar g <210O> 169 <21 1> 744 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (721) <223> RXA02477 <400> 169 cgagcagggc t gt t t gaaaa gct gt aaat g acat gacct a aat gat t gt a ct gact ggca ct t t aggt ca t at gt cacac cgagt ggaat aat aaagct t at g cct t t g cgt aat I'b t Pr o Leu Ar g Asn 1 gt t gat aga act ccg ccc gca gta tgg gaa gca t tg ct t gcc gga aac Val Asp Arg Thr Pr o Pro Ala Val Trp O31u Ala Leu Leu Ala O31y Asn 15 gaa aga ttc atc agt ttc aac gaa gat cga cca aac cag gac gcc ccg O31u Ar g Phe I Ie Ser Ph eAsn O31u Asp Ar g PraoAsn O31n Asp Ala Pr o 30 cgc aga aga gaa ct t cgc aat gga caa acg cct gca gct gt t gt t at t Ar g Ar g Ar g O31u Leu Ar g As n 31y O31n Thr Pr o Ala Ala Val Val Ilie Page 220 seql i st cor r ect ed. t xt tcc tgt tca gat tct cga gtg cca gtt gag Ser Cys Ser Asp Ser Arg Val Pro Val G u 60 ctc ggt gac ctc ttt gtt gtc cgt act gcc Leu y Asp Leu Phe Val Val Arg Thr Ala 75 gca gtg ctt gcg tcc atc gaa tac gcc act Ala Val Leu Ala Ser I e G u Tyr Ala Thr 95 ttg gtt atc gtc atg ggc cac gaa tcc tgt Leu Val Ile Val Met G y His u Ser Cys 105 110 gca gca gca ctt gaa ggc ggt gca ctt ccc Ala Ala Ala Leu u Gy Gy Ala Leu Pro 120 125 ttg gtt gaa aag gtt gca cca tcc att cta Leu Val G u Lys Val Al a Pro Ser Ile Leu 135 140 ctg agc tcc atc aag gaa ttc gag gaa cac Leu Ser Ser Ile Lys G u Phe G u G u His 150 155 aac caa ctg ttg tcc cgt tct cca gag att Asn G n Leu Leu Ser Arg Ser Pro G u lie 170 175 ggt gag ttg gga atc att ggt ttg cgc tac Gy u Leu Gy lie lie y Leu Arg Tyr 185 190 act gaa cct gta att agc aag aac gtg ggt Thr G u Pro Val lie Ser Lys Asn Val y 200 205 cct <210> 170 <211> 207 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 170 IVet Pro Leu Arg Asn Val Asp Arg Thr Pro 1 5 10 Leu Leu Ala y Asn G u Arg Phe Ile Ser Asn G n Asp Ala Pro Arg Arg Arg G u Leu 40 Ala Ala Val Val lie Ser Cys Ser Asp Ser 55 Ile Phe Asp Val y Leu y Asp Leu Phe 70 G u Ile Leu Asp G n Al a Val Leu Al a Ser 90 Pag at t SIle gga Gy gaa G u ggt Gy gga Gy gaa G u cac Hi s 160 cat Hi s cga at t I e gaa G u t cc Ser gca Al a ggc Gy gcc Al a 145 gtt Val cag G n ct c ttt Phe at c SIle at c SIle gtt Val t ac Tyr 130 aag Lys gt g Val aag Lys tct gac Asp ct c Leu ggc Gy gca Al a 115 caa G n gca Al a gca Al a gt c Val gac Asp 195 gtc Val gac Asp gtt Val 100 gca Al a cga Ar g gag G u acg Thr gaa G u 180 ggt G y Arg Leu Ser tagttttcgg tctgagattg Pro Phe Arg Ar g Val ie e 221 Al a Asn Asn Val Val G u Val G u Sy Pr o Ar g Tyr G u Ar g Thr G u Al a Thr Ser Al a G31y Al a 145 ValI O31n Le u G31y Al a 115 O31n Al a Al a ValI As p 1 95 Pr o Thr ValI G31y ValI 1 65 Thr Ar g Le u Al a Le u Le u 150 As n G31y Thr ValI Al a ValI 135 Ser O31n (31u (31u seql i st car r ect ed. t xt IlieVal Ntbt Gy His Ou 1 Al a Leu 03 u 03 y 03 y Al a 120 125 O31u Lys Val Al a Pr o Ser 140 Ser I Ie Lys O31u Phe O31u 1 Leu Leu Ser Ar g Ser Pr o 1 70 Leu 03 y I I e I I e 03 y Leu 1 Pr o Val I Ie Ser Lys As n 200 205 cys Pr o Le u Hi s I Ie 1 Ty r G31y <210O> 171 <21 1> 618 <21 2> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CDS <222> (595) <223> RXN00833 <400> 171 agcttt t tgcat gt gtcat a tcgtaccgt t t gcat aggcc t t t ct agcac caaaacaaaa ct ct ccct ag t at ggggt cc t gt t cgcgct t ggt gaacct tt t Ph e g ac As p t cc Ser t cc Ser gca Al a ct t Le u gt c ValI ggc G31y ct c Le u aag Lys5 g ac As p gca Al a tt c Ph e cca Pr o g aa (31u gag (31u tt c Ph e ggc G31y ct g Le u ct g Le u t cc Ser act Thr t t c Ph e cag O31n gt t ValI gaa O31u 75 ggc O31y gca Al a gct Al a aac As n ggc O31y t gt cys aac As n cgt Ar g tt c Ph e ac c Thr ct c Le u cgc Ar g 45 gca Al a ac c Thr tt c Ph e cgt Ar g t cc Ser gt c ValI aag Lys5 ac a Thr ac c Thr t gc cys t cc Ser 110 ggc O31y 15 aac As n ct t Le u t ca Ser gt g ValI t cc Ser 95 ac c Thr g aa O31u ac c Thr gt c ValI gt c ValI ct g Le u gca Al a tt c Ph e at g vb t 1 ct g Le u g aa O31u ct g Le u cgc Ar g t gc cys g aa O31u ggt (31y gct aaa aca Al a Lys Thr cca Pr o ct g Le u aac As n aag Lys5 at c O31y g aa (31u gt c ValI gag O31u tt c Ph e aac As n aag gag O31u 1 00 aac As n Page 222 seql i st cor r ect ed. t xt atc gtg ctc gaa ggc tca cca ctt aag ggt ctt ctt gca lie Val Leu G u Gy Ser Pro Leu Lys y Leu Leu Ala 120 125 130 atc gtc gtc gat gaa aac ggc aag gtt gct tac acc cag I e Val Val Asp u Asn y Lys Val Al a Tyr Thr n 135 140 145 gag atc ttc act gaa cct gat tac gac gct gca ctt gct G u Ile Phe Thr G u Pro Asp Tyr Asp Ala Ala Leu Ala 150 155 160 taatttactt cgctcagggg aat <210> 172 <211> 165 <212> PRT <213> Corynebacter i um gl ut am cum cgc agc gtc Arg Ser Val ttg gtt gat Leu Val Asp ggg ctg aac G y Leu Asn 165 <400> 172 IVet Al a Lys Thr His Phe n G y Asn G u Thr Ala Thr Ser 1 Leu G u Leu Ar g Cys G u Gy Leu Thr 145 Leu G n Gy I le Phe Ser SIle Asp 115 Ar g Leu Gy 5 Gy Val Pr o G u Asp Asn Gy Val Asp Asn 165 Al a Asp Thr Ser Al a Val 105 G u Asp Thr Phe G n Val G u 75 Gy Al a Ser Asn Pr o 155 Leu Ar g Al a Thr Phe Ar g Leu 125 Lys Tyr G y G u Asn Thr Leu Val Ser Val Val Leu Ser Al a Thr Phe G y Leu Al a Tyr Al a Al a 160 <210> 173 <211> 469 <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(469) <223> FRXA00833 <400> 173 agctttttgc atgtgtcata tcgtaccgtt tgcataggcc tgttcgcgct tggtgaacct Page 223 seqI i st car r ect ed. t xt t t t ct agcac caaaacaaaa ct ct ccct ag t at ggggt cc at g gct aaa aca cat I'bt Al a Lys Thr Hi s tt t Ph e g ac As p t cc Ser t cc Ser gca Al a ct t Le u gt c ValI at c I Ie ggc G31y ct c Le u aag Lys5 g ac As p gca Al a tt c Ph e cca Pr o ct c Le u 120 g aa G31u gag O31u tt c Ph e ggc G31y ct g Le u ct g Le u t cc Ser ggc G31y act Thr t t c Ph e cag O31n gt t ValI gaa G31u 75 ggc G y gca Al a t ca Ser gct Al a aac As n ggc G31y t gt cys aac As n cgt Ar g tt c Ph e t cc Ser gt c ValI aag Lys5 ac a Thr ac c Thr t gc cys t cc Ser 110 g aa G31u ac c Thr gt c ValI gt c ValI ct g Le u 80 gca Al a tt c Ph e Thr Ph e O31n ValI O31u 75 G31y Al a Ser ct g Le u g aa O31u ct g Le u cgc Ar g 65 t gc cys g aa G u ggt G31y Al a As n G31y cys As n Ar g Ph e cca Pr o ct g Le u aac As n 50 aag Lys5 at c G y g aa 0Ou Thr Le u Ar g Al a Thr Ph e Ar g gt c ValI 20 gag O31u tt c Ph e aac As n aag Lys5 gag G u 1 00 aac As n G31y As n Le u Ser ValI Ser Thr 99c G31y gt c ValI cca Pr o gag O31u g at As p aac As n ggc G31y O31u Thr ValI ValI Le u Al a Ph e <210O> 174 <21 1> 123 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 174 I'b t Al a Lys Thr Hi s Phe O31n G31y Asn 1 5 Leu Pr o O31n Val G31y Asp Asn Leu Ala O31u L eu 31y O31u Val Ser Ser Lys Asp 40 Leu Asn Ilie Phe Pr o Ser Val Asp Thr 55 Arg Lys Phe Asn O31u Ala Al a Ala Se r 70 Cys Ilie Ser Lys Asp Leu Pr o Phe Al a 31u 31y Ilie O31u Asn Val Thr Pr o Val 100 105 G31y O31u Asp Asn G31y I Ie Val Leu O31u 115 120 Page 224 seqi i st car r ect ed. t xt <210> 175 <211> 1146 <212> DNA <213> Cor ynebact er i ur gl ut ani cum <220> <221> CDS <222> (101)..(1123) <223> RXA01224 <400> 175 ttggcggcgg gaagttcagg cttgggggca aacagtgctt acggaagt ca t cct at ggca ggcgcgcct a ggat ggt gcc ggat t t t aga caaaaaact c acg Thr act Thr ttg Leu gag O31u gcg Al a gcg Al a ttg Leu ccc Pr o gcc Al a ccg Pr o 150 gt g Val gt g Val caa (31n aaa Lys gcg Al a ggt G31y aaa Lys aag Lys cgg Ar g aat Asn gt t Val 120 at c I Ie gac Asp ggc G31y ccg Pr o 31y 200 ccc Pr o gt c Val gt c Val gt c Val t ca Ser ttt Phe t gg Tr p t cc Ser gcc Al a ct g Leu gag O31u 170 gag O31u t ac Tyr gt g Val aat Asn at g lvb t ttt Phe gac Asp 75 ggc G31y gag O31u t gc Cys acc Thr gct Al a 155 gcg Al a cgc Ar g ct c Leu at t SI e gca Al a cct Pr a ggc G31y at t SI e ct c Leu gct Al a acc Thr gga G31y 140 gcg Al a ggt G31y gac Asp ccg Pr a gt c Val gca Al a ct t Leu 45 gt c Val gat Asp gat Asp aaa Lys ttt Phe 125 at t SI e cag O31n gg G31y ct g Leu ct c Leu 205 gcc Al a gca Al a gag (31u aac Asn gag (31u gag (31u ttt Phe 110 ggt G31y gag (31u aaa Lys cac Hi s aaa Lys 190 at c SI e ccg Pr a 15 gag O31u c ag O31n ct g Leu ct g Leu ccg Pr a 95 gag O31u at t SI e gcg Al a gct Al a cgc Ar g 175 acc Thr gca Al a at g lvb t gca Al a ct g Leu ttt Phe gcg Al a 80 acg Thr gcc Al a ttt Phe t gg Tr p ggc G31y 160 t ct Ser ct c Leu gcc Al a at g lvb t 1 gct Al a ggt G31y aaa Lys cgc Ar g gga G31y gt g Val gt t Val agc Ser gt g Val 145 gcc Al a acc Thr ct c Leu ggc (31y ggc G31y t cc Ser cag O31n ccg Pr a ttg Leu cct Pr a ct t Leu gct Al a 130 acg Thr aac Asn t gg Tr p gca Al a ggc (31y 210 ggc G31y ct c Leu gaa (31u cag O31n ttg Leu acg Thr gcc Al a 115 g aa O31u gt g Val gcg Al a t cc Ser gct Al a 195 ct t Leu ccg Pr a tgg ttg Leu acg Thr t cc Ser ccg Pr a 100 gct Al a g aa O31u acc Thr ct t Leu at t SI e 180 gt c Val t ca Ser agc atc ctt Ser lIe Leu gac Asp t cc Ser ttc Phe t ca Ser gat Asp t cg Ser gat Asp aag Lys ttc Phe aat Asn gt c Val 165 g aa O31u aaa Lys acc Thr Page 225 seqi i st car r ect ed. t xt t cc Ser t gt CYS 230 aac As n t ca Ser tt c Ph e cca Pr o g at As p 310 ggt G31y g ac As p t cc Ser gag O31u cgc Ar g cgt Ar g 280 at c I Ie gcc A a gca A a gca A a tt t Ph e tt g Le u 250 tt t Ph e aac As n t ct Ser t gc cys c ag gca A a t t g Le u 235 gac As p t cg Ser gag O31u t t a Le u ct g Le u 315 at a at t I Ie 220 ct t Le u gcc A a ggC G31y G31y cgt Ar g 300 gt a ValI ct g Le u tta gaa gcc ggc L eu G3 u A a G3 y ag c Ser gcc A a cgt Ar g tt a Le u 285 aag Ly s gga O31y g aa O31u g ac As p cca Pr o t at Ty r 270 ccc Pr o gt g ValI gt c ValI t ca Ser g aa O31u gca A a 255 gcc A a ccg Pr o gcg A a ggc O31y Le u 335 gcc A a 240 ctt Le u ag g Ar g tt g Le u gga O31y ct g Le u 320 ac a Th r gcc A a 225 ggc O31y ggt O31y gga O31y t ac Ty r agt Ser 305 g aa O31u cct Pr o ag c Ser ac c Th r tt g Le u gt g ValI cca Pr a 290 gca A a t cg Ser t cc Ser gct A a ag c Ser g aa O31u g aa O31u 275 t ac Ty r ggg O31y at t I Ie gct A a gcc A a t ca Ser t cg Ser 260 ac c Th r ct c Le u aac As n gcg A a tt g Le u 340 787 835 883 931 979 1 027 1 075 1123 1146 Lys G3 n I I e 330 t aat gt tggg gggagt gct t t ca <210O> 176 <21 1> 341 <21 2> PRT <21 3> Cor ynebact er i urn gI ut anri cumn <400> 176 l'bt Ser I I e Leu Asp Thr Leu Lys Thr 1 5 Ala G1y Gy Pro Ser Thr Pro Ala Leu 25 G31y Se r Leu G31y Phe L eu Ala 31y G31y 40 Lys O31n O31u Leu Ser O31u ValI Lys G31y 55 Arg Pro O31n Thr As p Ala Pro Lys Pr o 70 O31y Leu Leu Ser Ser Ala Phe Ar g O31n Val Pr o Thr Pr o As p Leu Ser As n G31y 100 105 Val Leu Ala Ala Lys Pr o Ala Val Phe 115 120 Ser Ala (3u (3u Phe Ala Ar g I Ie Lys 130 135 Pro Val I Ie 10 Val As n Ala Val l'bt Pr o Val Phe G31y Ser As p I I e Phe 03 y Leu 90 Trp O31u Ala Ser Cys Thr A a Thr (3 y 140 Page 226 ValI A a Le u ValI As p As p Lys Ph e 125 li1e A a A a (31u As n (31u (31u Ph e 110 G31y (31u Pr o (31u O31n Le u Le u Pr o Ou le A a seql i st cor r ect ed. t xt Val Thr Val Thr Asn Pro G u Asp Ala Leu Ala Ala G n Lys Ala 145 150 Al a Thr Leu Gy Al a 225 Gy Gy Gy Tyr Ser 305 G u Pr o Al a Ser Al a 195 Leu Al a Ser G u G u 275 Tyr Gy I e Al a Val 165 G u Lys Thr Ser Leu 245 Val Ar g Asn Tr p Lys 325 Gy G n G u Al a Al a 215 Gy Ar g Ser Thr Mbt 295 Tyr Ser Gy Pr o Gy 200 Asp Ser G u Ar g Ar g 280 I le Al a Al a Pr o Asp 185 Val Val Al a SIle Al a 265 Ser Thr Tyr Lys G u 170 G u Tyr Al a Phe Leu 250 Phe Asn Ser Cys G n 330 155 Al a Ar g Leu Al a Leu 235 Asp Ser G u Leu Leu 315 I le Gy Asp Pr o l Ie 220 Leu Al a Gy Gy Ar g 300 Val Leu Gy Leu Leu 205 Leu Ser Al a Ar g Leu 285 Lys Gy G u Ar g 175 Thr Al a Al a G u Al a 255 Al a Pr o Al a Gy Leu 335 <210> 177 <211> 516 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(493) <223> RXA01182 <400> 177 gttaaaacgg aaactaatac cccaaaggat accgattcaa tttgtgatgt gtggtgttcg ggtcatatca agctaaacag atgcccccta caataggctt gtg ttc aat tta ttt Val Phe Asn Leu Phe 1 ggt cgt aaa act cct cgc tct aac ctc cgc cca cca cgc ggt ccg ggc Sy Arg Lys Thr Pro Arg Ser Asn Leu Arg Pro Pro Arg Gy Pro Gy 15 gat act gtg cgc ccg gaa gat tta aaa ttc ttg atg caa tgg gtg cag Asp Thr Val Arg Pro G u Asp Leu Lys Phe Leu IVet G n Trp Val G n 30 gat aag cca ttt gtt gag gca ttc gtt gaa ccg gaa acg ctg gtc aat Asp Lys Pro Phe Val G u Ala Phe Val G u Pro G u Thr Leu Val Asn Page 227 seql i 45 gag atg tct gtc gtt ttg gtt gat gct G u let Ser Val Val Leu Val Asp Ala 60 agg atc ggc ggt ccc aaa ggg att gat Arg lie Gy y Pro Lys Gy Ile Asp 75 gtt ccg gtt tat gat gtt gag gag acc Val Pro Val Tyr Asp Val G u G u Thr gaa cgc att gaa tat gag cgc atc tta G u Arg lie G u Tyr G u Arg lie Leu 105 110 gct cgc cgc gct aaa ttt gag cgc ggc Ala Arg Arg Ala Lys Phe G u Arg y 120 125 taactagcgt ttagctttcc gac <210> 178 <211> 131 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 178 Val Phe Asn Leu Phe G y Arg Lys Thr 1 5 Pro Arg y Pro y Asp Thr Val Arg IVet G n Trp Val G n Asp Lys Pro Phe 40 G u Thr Leu Val Asn G u lMt Ser Val 55 Val Phe Val Arg Arg Arg Ile y y 70 Ala Lys Lys Leu G y Val Pro Val Tyr Pro n Arg IVet Arg u Arg lie u 100 105 Arg u u n Lys Al a Arg Arg Ala 115 120 Pro Asp Leu 130 <210> 179 <211> 834 <212> DNA <213> Cor ynebact er i um gl ut ai cum <220> <221> CDS <222> (101)..(811) <223> RXA02531 st cor r ect ed. t xt cat ggg gtt ttt His G y Val Phe gtt atc gcg aaa Val I e Ala Lys 80 ggt tac ccc caa Sy Tyr Pro n 95 aga aag cgt gag Arg Lys Arg G u gag aat cct gat G u Asn Pro Asp 130 gt c Val aag Lys agg Ar g gaa G u 115 ctt Leu cgc cga Arg Arg ctc ggc Leu G y atg cgc IVet Arg 100 caa aaa G n Lys Ar g G u G u Leu Lys Val G u Phe Ser Asp Al a Val a y G u Ar g G u Asn Leu Phe Asp SIle G u SIle Ar g 125 Ar g Phe G u Hi s Val Sy Ar g G u Page 228 seql i st cor r ect ed. t xt <400> 179 -i cacttcgctc cccaaggtac atccccgatg ccacttcttg gagccatcat cggtgccacc C aaacacattg aagtgggcac tggagtagtg gatatgcgtt atg aaa atc cct ttg 115 Ibet Lys Ile Pro Leu 1 tat atg gcc gag gaa gca gct gct ctc aat ctg ctt gcc gac ggc cga 163 Tyr IVet Ala G u G u Al a Ala Ala Leu Asn Leu Leu Ala Asp y Arg 15 CK1 cta gcc ctc gga gtt tcc agg gga tca ccc gaa cca gcc gag aag ggt 211 0 Leu Ala Leu y Val Ser Arg y Ser Pro G u Pro Ala G u Lys y 00 25 30 0 St gg gaa gct t t c ggc tac gac ggc ggt gat gat cct aaa gct gca ggc 259 Cr Tr p u Ala Phe G y Tyr Asp G y G y Asp Asp Pro Lys Ala Al a y 40 45 0 atg gca cgg gag aaa ttc ctt cgc ttc ctc gat gcc atc gat ggt cgc 307 CK IVet Al a Arg G u Lys Phe Leu Arg Phe Leu Asp Al a lIe Asp G y Arg 60 ccc atg tcc atc gct tcc gag aat caa tac cca cgc ctc tac cat ccg 355 Pro IVet Ser Ile Ala Ser G u Asn G n Tyr Pro Arg Leu Tyr His Pro 75 80 ggc act ccc ctg ccg atc ttc ccg cat gat ctt gac ttg ggt aaa tcc 403 G y Thr Pro Leu Pro IIe Phe Pro His Asp Leu Asp Leu G y Lys Ser 95 100 att tgg tgg ggc gcc ggt tcc cac aac acc gcc gaa caa gca gca cgc 451 IIe Trp Trp G y Ala G y Ser His Asn Thr Ala G u G n Ala Ala Arg 105 110 115 gat ggc gtt aac ttg atg agc tcc acc ctc gtc gcc gaa gcc acc ggc 499 Asp y Val Asn Leu IVet Ser Ser Thr Leu Val Ala G u Ala Thr y 120 125 130 caa tcc ttc ggg gat ctg caa gcc gat caa atc gcg ttc tac cgc caa 547 Sn Ser Phe y Asp Leu n Ala Asp n Ile Ala Phe Tyr Arg n 135 140 145 gct tgg aaa gaa gcc gga cac gat tgg acc cca cgt gtg tct gtc tcc 595 Ala Trp Lys G u Ala G y His Asp Trp Thr Pro Arg Val Ser Val Ser 150 155 160 165 agg tcc atc ttt ccg atc gtc acc gac cgc gac cgt gag ctt ttc gga 643 Arg Ser Ile Phe Pro Ile Val Thr Asp Arg Asp Arg G u Leu Phe y 170 175 180 ctt cag gga caa ggc ggt gac caa gta gga atc ctg gat gat acc cga 691 Leu Gn Gy On Gy Gy Asp Gn Val Gy Ile Leu Asp Asp Thr Arg 185 190 195 tcc acg ttc ggt cgc agc tac gcc gga agt ccc gat gaa ctc atc gac 739 Ser Thr Phe y Arg Ser Tyr Ala y Ser Pro Asp G u Leu Ile Asp 200 205 210 cag ctc caa gga aga caa agc tgt gat gga agc cga cac ctt gat gct 787 G n Leu G n y Arg G n Ser Cys Asp G y Ser Arg His Leu Asp Ala 215 220 225 cac cgc ccc caa cca aat ggg tgt tgagatcaac gcgtcgatcc tga 834 His Arg Pro G n Pro Asn G y Cys 230 235 Page 229 seqi i st car r ect ed. t xt <210O> 180 <21 1> 237 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 180 l'bt Lys I Ie Pro Leu Tyr l'bt Al a a u au Al aAl aAl a Leu Asn Leu Le u Pr o Pr o Al a Ar g As p O31u Al a Al a 145 Ar g Ar g Le u As p Ar g 225 As p O31u Al a As p Tyr (31y Al a 115 Al a Tyr Ser Le u As p 1 95 Le u Le u Ar g G31y G31y Ar g Pr o Ser Ar g G31y O31n Ser 1 65 G31y Ar g As p Al a Al a O31u Al a vb t Thr Tr p G31y Ser 135 Tr p Ser O31n Thr Le u 215 Ar g Le u Al a 40 Ar g Ser Pr o Tr p ValI 120 Ph e Ly s I Ie G31y Ph e 200 O31n Pr o G31y Ph e (31u I Ie Le u G31y 1 As n G31y (31u Ph e O31n 1 O31y O31y O31n 10 ValI O31y Ly s Al a Pr a 90 Al a Le u As p Al a Pr a 1 70 O31y Ar g Ar g Pr a Ser Tyr Ph e Ser 75 li1e G y vb t Le u O3y 1 55 le Oy Ser O3n As n 235 O31y O31y Ar g As n Pr a Hi s Ser 125 Al a As p Thr O31n Al a 205 cys Cys Pr a As p Le u Tyr As p Thr Le u O31n Thr Ar g 1 O31y Ser O31y O31u As p As p Pr a Le u Al a ValI I Ie Pr a 1 As p I Ie Pr a Ser <210O> 181 <21 1> 1614 <212> DNA <213> Cor ynebact er i urn gi ut ani curn <220> <221 ODS <222> (1591) <223> RXN00689 <400> 181 acagggaaat cctcccagaa ttaatcaccg aagctgcaca ccagatggct actgcagacc t caat cgt gc aaaggccct g t taagaacgg at gcgat ccg at g aat gct gca acc 115 l'bt Asn Al a Al a Thr Page 230 seql i st cor r ect ed. t xt agg Ar g at c l Ie gtt Val gca Al a tcg Ser at c SIle acc Thr aat Asn gac Asp gt g Val 150 ttc Phe ggc Gy cgt Ar g gt c Val cac Hi s 230 aag Lys gag G u gct Al a ggt Gy cct Pr o gaa G u agt Ser acg Thr gaa G u gca Al a 120 gaa G u aaa Lys at c SIle acc Thr at c SIle 200 ct a Leu gga Gy at c SIle ggc Gy ct g Leu tgg Tr p gt c Val gac Asp ctt Leu at t SIle ggt Gy at t l Ie at c SIle ccc Pr o ctt Leu 170 at c SIle gaa G u acc Thr gac Asp gcc Al a 250 aaa Lys caa G n gtt Val ggt Gy tct Ser aca Thr 75 gaa G u t cc Ser tt c Phe cgc Ar g at c SIle 155 gt a Val at c SIle gcc Al a ggt Gy aag Lys 235 gcc Al a tct Ser ct c Leu aaa Lys caa G n gcg Al a ggt Gy t cc Ser ccc Pr o cgt Ar g cca Pr o 140 ggt Gy gt c Val aaa Lys at c l Ie t ca Ser 220 gt a Val tgc Cys t cc Ser ccc Pr o gca Al a gaa G u 45 gt g Val gcg Al a cgt Ar g at t SIle t ac Tyr 125 ttt Phe gt c Val at c SIle cca Pr o gaa G u 205 ggg Gy gcg Al a gga Gy gcg Al a t at Tyr gaa G u tgg Tr p gga Gy gaa G u tct Ser t cc Ser 110 ttt Phe cct Pr o tgc Cys aaa Lys gcc Al a 190 gcc Al a cgt Ar g ttt Phe gaa G u at t acc Thr 15 gga Gy gga Gy gct Al a cga Ar g gaa G u 95 gag G u gcc Al a gcc Al a gca Al a ct g Leu 175 t cc Ser gcc Al a ttc Phe acc Thr ct a Leu 255 cat Hi s aca Thr tct Ser gca Al a aca Thr 80 gct Al a acc Thr act Thr ggt Gy ct c Leu 160 gca Al a ccc Pr o gga Gy ggt Gy gga Gy 240 ct c Leu 1 gt c Val caa G n gtt Val cgt Ar g ggc Gy ct a Leu cgt Ar g ct c Leu agc Ser 145 at c SIle cca Pr o acc Thr gt g Val gat Asp 225 t ca Ser cga Ar g gat Asp cgc Ar g cca Pr o aca Thr t ac Tyr gca Al a gga Gy gcg Al a 130 gcc Al a gcc Al a gca Al a cca Pr o cca Pr o 210 gcc Al a acg Thr cca Pr o gat Asp aac Asn gaa G u gcg Al a ct c Leu ctt Leu gct Al a 115 cct Pr o gaa G u cca Pr o ctt Leu ct g Leu 195 gca Al a ctt Leu cct Pr o gt g Val ttc Phe ccc Pr o gca Al a ct a Leu ct g Leu act Thr 100 gcg Al a tgg Tr p t cc Ser tgg Tr p ctt Leu 180 tcg Ser ggc Gy gt c Val gtt Val act Thr 260 t ac Tyr gta Val acc Thr aag Lys aaa Lys aat Asn t cc Ser tta Leu at c SIe aat Asn 165 gcc Al a at c SIe gta Val cgc Ar g gga Gy 245 tta Leu atc ctt cct gat gca gac atg Asp Ala Asp IVet lie Ile Leu Pro Page 231 seql i st cor r ect ed. t xt 270 t ca Ser caa G n gcg Al a 310 ggt Gy tct Ser ggt Gy ggt Gy acc Thr 390 t cc Ser ct a Leu gat Asp gt a Val cgc Ar g 470 gct Al a ct c Leu 280 tgc Cys gt c Val ccc Pr o t ac Tyr cga Ar g 360 gaa G u gac Asp ct a Leu aac Asn gaa G u 440 at c SIle gaa G u ct g Leu acg Thr at c SIle caa G n gat Asp 330 acc Thr gtt Val ggc Gy cgg Ar g t ac Tyr 410 acg Thr gca Al a ttc Phe ggt Gy t ac Tyr 490 cgg Ar g agt Ser aca Thr 315 gaa G u gt c Val gca Al a gag G u at a SIle 395 gac Asp aaa Lys ct a Leu tt c Phe at g Met 475 ttg Leu acc Thr 300 gt g Val gaa G u at g let ggt Gy ttt Phe 380 t ca Ser gat Asp ttc Phe gaa G u ggt Gy 460 gga Gy cga Ar g at t SIle agc Ser gtt Val t ac Tyr 350 acc Thr caa G n gaa G u aac Asn ct c Leu 430 gcc Al a aac Asn gaa G u t cc Ser at t SIle act Thr ttt Phe 335 at t SIle cgg Ar g cca Pr o gaa G u ggt Gy 415 ggt Gy cgt Ar g cat Hi s t ac Tyr cga Ar g 495 tgt Cys gcc Al a at c SIle 320 ggg Gy gac Asp t ca Ser acc Thr at c SIle 400 gtt Val ggc Gy caa G n t cc Ser ggc Sy 480 at g Met cct Pr o 305 gct Al a cca Pr o t cc Ser at c SIle gt g Val 385 ttc Phe t cc Ser ttg Leu gt g Val gcc Al a 465 at c SIle cgc Ar g 290 agc Ser gca Al a gtt Val gca Al a agc Ser 370 ttt Phe ggc G y gaa G u gt a Val gat Asp 450 cca Pr o gaa G u aac Asn t ca Ser ggt Sy gcc Al a cga Ar g 355 ctt Leu gcc Al a cct Pr o gca Al a ttt Phe 435 t ct Ser ttt Phe ggc Sy act gga Thr G y cgc t at Arg Tyr aga caa Arg G n 325 agc gcc Ser Al a 340 gag gaa G u G u tct gaa Ser G u gat gtc Asp Val gtt att Val lie 405 atc gca I e Al a 420 ggt gcg G y Al a ggt tcc G y Ser gga gga G y G y ct c agt Leu Ser 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1601 1614 aag agt att cac Lys Ser Ile His acc att tagttactga Thr Ile aagt t ctcag ct a <210> 182 <211> 497 <212> PRT <213> Corynebacter i um gl utam cum <400> 182 lIet Asn Ala Ala Thr Arg Arg Ala Ser Leu n Leu Pro Tyr Thr His Page 232 seql i st cor r ect ed. t xt Tyr Ile Asn Gy Val G n Val Ar g Gy Leu Ar g Leu Ser 145 I le Pr o Thr Val Asp 225 Ser Ar g Pr o Met Pr o 305 Al a Pr o Ser Asp Asn G u Al a Leu Leu Al a 115 Pr o G u Pr o Leu Leu 195 Al a Leu Pr o Val Al a 275 Asn Ser Gy Al a Ar g 355 Val Thr Lys Lys Asn Ser Leu SIle Asn 165 Al a SIle Val Ar g Gy 245 Leu lbet Gy Tyr G n 325 Al a G u Val Al a Ser 70 I le Thr Asn Asp Val 150 Phe Gy Ar g Val Hi s 230 Lys G u Ser G n Al a 310 Gy Ser Gy Asp Ser Tr p Al a Ar g Al a Gy 135 Asp Pr o Cys Phe Asn 215 Pr o Lys Leu Val Thr 295 G u Asp G n Al a Ser Al a Leu Al a G u Asn 105 Gy Asp Asp Asn Val 185 I le Leu Val Al a Gy 265 Ser Tyr Val Tyr Ser 345 Val Trp Val Val G y Asp Ser Leu Thr 75 Ile u 90 G y Ser Ile Phe li e Ar g Pro Ile 155 Leu Val 170 lie Ile G u Al a Thr G y Asp Lys 235 Al a Al a 250 Lys Ser Thr Arg Ile Ser G n Thr 315 Asp u 330 Thr Val Lys Al a G u Sn u Trp Ala Val y G y Al a G u Ser Arg Ser Pro Ile Ser 110 Arg Tyr Phe 125 Pro Phe Pro 140 G y Val Cys Val Ile Lys Lys Pr o Al a 190 l e G u Ala 205 Ser G y Arg 220 Val Al a Phe Cys G y u Ser Al a li e 270 Leu II e Ar g 285 Thr Arg Il e 300 Val Al a Ser G u Thr Val IVet Ser Tyr 350 Gy G y Al a Ar g G u G u Al a Al a Al a Leu 175 Ser Al a Phe Thr Leu 255 I le Ser SIle Thr Phe 335 I le Thr Ser Al a Thr Al a Thr Thr Gy Leu 160 Al a Pr o Gy Gy Gy 240 Leu Leu Cys Al a SIe 320 Gy Asp Val Ala G y Gy 365 Thr Arg Ser Page 233 SIle Val 385 Phe Ser Leu Val Al a 465 I le SIe Leu Al a Pr o Al a Phe 435 Ser Phe Gy G u Val SIle 405 Al a Al a Ser Gy Ser 485 Leu 375 Pr o SIle Al a G u Gy 455 Hi s Tyr seql i st cor r ect ed. t xt Su Ser G y u Phe Ile 380 Asp IVet Arg lie Ser Arg 395 Leu Lys Tyr Asp Asp Thr 410 Asn Asn Thr Lys Phe G y 425 G u G n Al a Leu G u Val 440 445 I le Asn Phe Phe G y Ser 460 Su Ser y e t y Val 475 Leu Thr Tyr Lys Ser Ile 490 Pr o G u Sy 415 Sy Ar g Hi s Tyr Ar g 495 <210> 183 <211> 750 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(727) <223> FRXA00689 <400> 183 actccgacca gtgactttag agctaggcgg aaa agacat gt ca gt act ct cga cgcggt t gat t cg caa G n gcg Al a ggt Sy tct Ser ggt Sy ggt Sy tgc Cys gt c Val ccc Pr o t ac Tyr cga Ar g gaa Gu at c I e caa G n gat Asp acc Thr gtt Val ggc G y agt Ser aca Thr gaa G u gt c Val gca Al a 75 gag G u acc Thr gt g Val gaa G u at g Met ggt G y ttt Phe cgg Ar g gca Al a acg Thr 45 tct Ser gga G y at c SI e at t SIle agc Ser gtt Val t ac Tyr acc Thr caa G n latcttcc gcgattatcc ttcctgatgc atcctgt atg cgc aac act gga IVet Arg Asn Thr y 1 att gcc cct agc tca cgc tat lie Ala Pro Ser Ser Arg Tyr 15 act atc gct gca ggt aga caa Thr Ile Ala Ala y Arg n ttt ggg cca gtt gcc agc gcc Phe y Pro Val Ala Ser Ala att gac tcc gca cga gag gaa Ile Asp Ser Ala Arg u u cgg tca atc agc ctt tct gaa Arg Ser Ile Ser Leu Ser G u 80 cca acc gtg ttt gcc gat gtc Pro Thr Val Phe Ala Asp Val 95 100 Page 234 acc ccc gac atg cgg ata tca Thr Pro Asp IVet Arg Ile Ser 105 t cc at ct a aag tac gac gat Ser Ile Leu Lys Tyr Asp Asp 120 cta gcc aac aac acg aaa ttc Leu Ala Asn Asn Thr Lys Phe 135 140 gat gag gaa caa gca cta gaa Asp G u G u n Ala Leu G u 150 155 gta ggc atc aac ttc ttc ggt Val y Ile Asn Phe Phe y 170 cgc cac gaa tcc ggt atg gga Arg His u Ser G y IVt G y 185 gct tac ctg aca tac aag agt Ala Tyr Leu Thr Tyr Lys Ser 200 aagt t ctcag cta <210> 184 <211> 209 <212> PRT <213> Cor ynebact er i um gl ut ar seql i st cor r ect ed. t xt cgc gaa gaa atc ttc ggc cct gtt Arg u u lie Phe y Pro Val 110 115 aca aac ggt gtt tcc gaa gca at c Thr Asn y Val Ser u Ala lie 125 130 ggt ctc ggt ggc ttg gta ttt ggt Sy Leu G y y Leu Val Phe y 145 gtc gcc cgt caa gtg gat tct ggt Val Ala Arg G n Val Asp Ser y 160 tcc aac cat tcc gcc cca ttt gga Ser Asn His Ser Ala Pro Phe y 175 180 gtg gaa tac ggc atc gaa ggc ctc Val u Tyr y lie u y Leu 190 195 att cac cga acc att tagttactga Ile His Arg Thr Ile 205 att SIe gca Al a gcg Al a t cc Ser 165 gga G y agt Ser <400> 184 IVet Arg Asn Thr 1 Pro Ser Ser Arg Ala Ala y Arg Pro Val Ala Ser Ser Ala Arg G u lie Ser Leu Ser Val Phe Al a Asp 100 Phe y Pro Val 115 Ser G u Ala li e 130 Leu Val Phe G y 145 Sy 5 Tyr G n Al a G u G u Val SIle Al a Al a Sn Thr Cys Tyr lie Ser Thr Al a Sy Ser Sy 70 Sy Thr Ser Leu Asp 150 G u Asp G n Al a Leu Pr o SIle Al a 135 G u Val Tyr Ser Val Ser Met 105 Lys Asn G n 10 G n Thr Asp Gu Thr Val I Val Ala G y G uI 90 Arg Ile Tyr Asp Thr Lys I Al a Leu 155 Page 235 Ar g Al a Thr Ser Sy SIle Ar g Thr 125 Sy Val I e Thr Phe SIe Ar g Pr o a u Sy Sy Ar g INO seql i st cor r ect ed. t xt Val Asp Ser y Ser Val Gy I e Asn Phe Phe G y Ser Asn His Ser 165 170 175 Ala Pro Phe G y G y Arg Hi s u Ser G y Met G y Val G u Tyr G y 180 185 190 d) SI e G u G y Leu Ser Ala Tyr Leu Thr Tyr Lys Ser lie His Arg Thr 195 200 205 lie 0 <210> 185 00 <211> 878 0 <212> DNA S<213> Cor ynebact eri um gl ut ari cum <220> 0 <221> CDS 0 <222> C( <223> RXN03128 <400> 185 aac gga ctc gcc att ccc gac att gga ttt ggt gta ttc caa acc cca 48 Asn G y Leu Ala lie Pro Asp Ile Gy Phe G y Val Phe G n Thr Pro 1 5 10 ccc gat gaa acc cga aac tcc gtt aac gct gct ctt gaa gcc ggc tat 96 Pro Asp G u Thr Arg Asn Ser Val Asn Ala Ala Leu u Al a y Tyr 25 cgc cac atc gac acc gcc gcc gca tac ggc aat gaa cgt gaa gtc ggt 144 Arg His lie Asp Thr Al a Al a Ala Tyr G y Asn G u Arg G u Val G y 40 gaa gca atc gca gca tcc ggc att ggc cgc gac gag atc acc atc gaa 192 Su Ala Ile Al a Ala Ser Gy lie y Arg Asp u Ile Thr Ile u 55 acc aaa atc tgg gtg acc gac tac ggc ttc gag gaa act ctc cac gca 240 Thr Lys IIe Trp Val Thr Asp Tyr G y Phe G u G u Thr Leu His Ala 70 75 ttc gac aag gcc aca ggc aag ctt ggt gtc gat aca ctg gac att ttg 288 Phe Asp Lys Ala Thr G y Lys Leu y Val Asp Thr Leu Asp Ile Leu 90 atc ttg cac cag gca gtg cca agc agc ttt gat cgc acc atc gcc gcc 336 Ile Leu His G n Ala Val Pro Ser Ser Phe Asp Arg Thr lie Ala Ala 100 105 110 tac aag gcg cta gag aag ctg ctt ttc gac ggc gcg gtg cgg gca atc 384 Tyr Lys Ala Leu G u Lys Leu Leu Phe Asp y Ala Val Arg Ala Ile 115 120 125 gga gtc agt aat ttc atg cca gag cac ctg gac aaa ctc ctt ttg gaa 432 G y Val Ser Asn Phe IVet Pro G u Hi s Leu Asp Lys Leu Leu Leu G u 130 135 140 acc tcc att gtc cca gct ctg aac caa atc gaa tgc cac ccc tac ttc 480 Thr Ser Ile Val Pro Al a Leu Asn n lie G u Cys His Pro Tyr Phe 145 150 155 160 cag cag cgt gac gtg ctt gcc cgc aat gag cag ctt ggc att ttg act 528 G n n Arg Asp Val Leu Ala Arg Asn G u G n Leu Gy Ile Leu Thr 165 170 175 Page 236 cag gcg t gg t ca O31n Al a Tr p Ser 1 80 ct t cca agc act Leu Pro Ser Thr 1 95 gtt ggc aaa aca Val 03 y Lys Thr 210 aga cgt cac gca Ar g Ar g Hi s Al a 225 aac t tt gag at c Asn Phe 03 u Il e at c gat gcc ct c Il e Asp Al a Leu 260 at c acc at g gaa Ilie Thr [Vb t G u 275 t agaggcgaa t gt <210> 186 <211> 285 <212> PRT <213> Cor ynebact <400> 186 As n 03 y Leu Al a Pr o Asp O31u Th r Arg His IlieAsp O u Al a IlIe Al a Thr Lys I Ie Tr p Phe Asp Lys Al a Il e Leu Hi s 03 n 1 00 Tyr Lys Al a Leu 115 G31y Val Ser Asn 130 Thr Ser Ilie Val 145 cca Pr o ct a Le u cca Pr o at t I Ie tt t Ph e 245 aac As n aac As n at c I Ie gaa G31u gct Al a cca Pr o 230 g at As p acc Thr t ac Tyr ggt G31y aat As n c aa O31n 215 aag Lys5 tt c Ph e g at As p t ac Tyr seqI i st car r ect ed. t xt ggc at c acc t tc t ac cgc G31y I Ie Thr Phe Tyr Ar g 1 85 gag gt c at c gct gga at c 31u Val I Ie Al a 1y I Ie 200 205 gt a at g ct g cgc t gg cac Val I'b t Leu Ar g Tr p Hi s 220 tct gtg acc cca tca cgc Ser Val Thr Pr o Ser Ar g 235 gaa ctc tcc gat gag caa O3 u Leu Ser Asp 03 u 03 n 250 ct g cgc ggt ggc cca gaa Leu Arg G31y G31y Pro O31u 265 cga gaa at c cca gaa gcc Ar g G u Ilie Pr o G u Al a 280 285 gac gga cag Asp G31y O31n 1 gcc gca gaa Al a Al a (3 u ct a cag cgt Leu O31n Ar g at t gt g gaa Ilie Val O31u 240 cta cag caa Leu 03 n 03 n 255 cca gag aac Pro O31u Asn 270 t aaaggccct er i urn gi ut anm cumn le Ar g Thr Al a ValI Thr Al a O31u Ph e Pr o Pro Asp Ilie Oy As n Al a Ser Thr 70 O31y ValI Lys5 vb t Al a 150 Ser Al a O31y 55 As p Lys5 Pr a Le u Pr a 135 Le u ValI Al a 40 li1e Tyr Le u Ser Le u 120 O31u As n As n 25 Tyr O31y O31y O31y Ser 1 05 Ph e Hi s O31n Ph e 10 Al a O31y Ar g Ph e ValI 90 Ph e As p Le u I Ie O31y Al a As n As p O31u As p As p O31y As p O31u 1 ValI Le u O31u O31u O31u Thr Ar g Al a Lys5 140 Cys Ph e O31u Ar g li1e Thr Le u Thr ValI 125 Le u Hi s O31n Al a O31u Thr Le u As p I Ie 110 Ar g Le u Pr a Thr O31y ValI I Ie Hi s I Ie Al a Al a Le u Tyr On OnArgAspVai LeuAiaArgAsn Ou On Leu Oy Ile LeuThr Page 237 O31n Le u ValI Ar g 225 As n I Ie I Ie Tr p Ser 1 95 Ly s Hi s O31u A a vb t 275 1 65 Pr o Le u Pr o I Ie Ph e 245 As n As n I e G31u A a Pr o 230 As p Thr Tyr G31y As n O31n 215 Ly s Ph e As p Tyr seqi i st car r ect ed. t xt 1 70 G31y I Ie Thr Ph e Tyr Ar g 1 85 31u Val I Ie A a (1y I Ie 200 205 Val l'b t Leu Ar g Tr p Hi s 220 Ser Val Thr Pr o Ser Ar g 235 O3 u Leu Ser As p 03 u 03 n 250 Leu Ar g 31y G31y Pro O31u 265 Ar g O31u I Ie Pr o O31u Ala 280 285 As p 1 A a Le u I Ie Le u Pr o 270 1 G31y A a O31n ValI O31n 255 O31u <210O> 187 <21 1> 522 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (522) <223> FRXA02192 <400> 187 at t le cga Ar g ac c Th r gca A a gt g ValI ac a Thr gca A a gag (31u g ac As p t cc Ser gcc A a ggc O31y g ac As p aag Ly s cca Pr a ct g Le u 115 gga O31y aac As n t ac Tyr O31y O31y ggt O31y ag c Ser tt c Ph e tt t Ph e gct A a ggc O31y cgc Ar g t t c Ph e 70 gt c ValI tt t Ph e gac As p ggt O31y gct A a aat As n g ac As p gag O31u g at As p g at As p ggc (31y gt c ValI ct t Le u g aa O31u 40 gag O31u g aa O31u ac a Thr cgc Ar g gcg A a 120 tt c Ph e g aa O31u 25 cgt Ar g at c I Ie act Th r ct g Le u ac c Th r 1 05 gt g ValI c aa O31n gcc A a g aa O31u ac c Thr ct c Le u g ac As p 90 at c Ar g ac c Thr ggc O31y gt c ValI at c I Ie c ac Hi s 75 at t I Ie gcc A a gca A a cca Pr a t at Tyr ggt O31y g aa O31u gca A a tt g Le u gcc A a at c I Ie ccc Pr a cgc Ar g g aa O31u ac c Thr tt c Ph e at c I Ie t ac Tyr gga O31y 125 g at As p c ac Hi s gca A a aaa Ly s g ac As p tt g Le u aag Ly s 110 gt c ValI g aa O31u at c I Ie at c I Ie at c I Ie aag Ly s c ac Hi s gcg A a agt Ser ac c Thr g ac As p gca A a t gg Tr p gcc A a c ag O31n ct a Le u aat As n t tc at g cca gag cac ct g gac aaa ct c ct t t tg gaa acc t cc at t gt c Page 238 Phe Met Pro 130 cca gct ct g Pro Al a Leu 145 gtg ctt gcc Val Leu Al a Hi s caa G n aat Asn 165 Asp 135 gaa G u cag G n seql i st cor r ect ed. t xt Lys Leu Leu Leu G u Thr Ser lie Val 140 tgc cac ccc tac ttc cag cag cgt gac Cys His Pro Tyr Phe n G n Arg Asp 155 160 ctt ggc att ttg act cag gcg Leu G y Ile Leu Thr G n Al a 170 <210> 188 <211> 174 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 188 Ile Pro Asp Ile 1 Ar g Thr Al a Val Thr Al a G u Phe Pr o 145 Val Ser Al a Sy Asp Lys Pr o Leu 115 Pr o Leu Al a Sy 5 Asn Tyr Sy Gy Gy Ser Phe Hi s G n Asn 165 Phe G y Val Al a Gy Ar g Phe 70 Val Phe Asp Leu SIle 150 G u Al a Asn Asp G u Asp Asp Gy Asp 135 G u G n Phe G n 10 G u Al a Arg G u Ile Thr Thr Leu Leu Asp 90 Thr Ile 105 Val Arg Leu Leu His Pr o G y lle 170 Thr Sy Val SIle Hi s 75 I le Al a Al a Leu Tyr 155 Leu Pr o Tyr Sy G u Al a Leu Al a l Ie G u 140 Phe Thr Pr o Ar g G u Thr Phe SIle Tyr Sy 125 Thr G n G n Asp Hi s Al a Lys Asp Leu Lys 110 Val Ser G n Al a G u I e I e I e Lys Hi s Al a Ser SIle Ar g Thr Asp Al a Tr p Al a G n Leu Asn Val Asp 160 <210> 189 <211> 1039 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1039) <223> RXA02351 <400> 189 tgacacttta cagactggtt ttcaactaat gacaccgaaa gaaatacacc tcaacctttt tgctttcggt gccgggcacc acgcggcggc gtggcgagcg gtg gag gga agc gtc 115 Val G u Gy Ser Val 1 Page 239 seql i st cor r ect ed. t xt gaa aag ctg ggt G u Lys Leu G y gag G u aat Asn acc Thr agc Ser at c SIle gt g Val ttg Leu acc Thr gat Asp 150 cat Hi s cct Pr o gga G y tgg Tr p gcc Al a 230 act Thr ctt Leu ggc G y gt c Val ttg Leu at t SIle agc Ser tcg Ser ggt Gy 120 act Thr gct Al a ggt Gy cag G n gaa G u 200 ttg Leu gcc Al a gtt Val gcg Al a tta Leu ct g Leu ct g Leu gcg Al a agt Ser gat Asp acc Thr gat Asp ct g Leu aaa Lys ttc Phe 170 cga Ar g gct Al a caa G n ggt Sy acg Thr 250 ct g Leu att tcc tgg I e Ser Tr p gat Asp gag G u at g Met acg Thr 75 cat Hi s gat Asp gtt Val tgg Tr p ttt Phe 155 tt c Phe ccc Pr o gcg Al a gcg Al a cgc Ar g 235 acc Thr ccg Pr o gcg Al a aat Asn gcg Al a ttt Phe at t SIle gcg Al a cgc Ar g gat Asp 140 gcg Al a caa G n gt a Val aaa Lys caa G n 220 gag G u gt g Val gt c Val tgg gag gaa ctc gcg G u Leu Ala gt c Val ggg G y 45 cgg Ar g tgg Tr p tcg Ser gag G u t at Tyr 125 tct Ser gac Asp gt c Val ctt Leu t ac Tyr 205 gat Asp ccc Pr o gaa G u aaa Lys Irp ttt Phe ccg Pr o gcg Al a cag G n ggt G y gcg Al a 110 gcg Al a tgg Tr p t cc Ser gct Al a ttt Phe 190 gcc Al a t at Tyr at g Met gaa G u gac Asp 270 Su 15 ttg Leu ggc G y acg Thr ccg Pr o ggg G y 95 cgt Ar g cgc Ar g cct Pr o tcg Ser ggt G y 175 cag G n gag G u cgc Ar g ccg Pr o gcg Al a 255 t ca Ser gcc Al a tgg Tr p aac Asn ttt Phe 80 cgt Ar g aac Asn gct Al a gcg Al a ct c Leu 160 ccg Pr o gct Al a gca Al a tct Ser gt g Val 240 cgt Ar g ct a Leu gat Asp ttt Phe aat Asn cat Hi s gct Al a cac Hi s gcg Al a gaa G u 145 at t SIle ct g Leu gga G y at t l Ie gat Asp 225 ctt Leu gca Al a aat Asn G y ttg Leu at t SIle gcg Al a gga G y ggg G y gaa G u 130 agt Ser aaa Lys aat Asn t cc Ser t ac Tyr 210 at t SIle cct Pr o aaa Lys cag G n cgc acc Arg Thr cag gcg G n Al a gag ccg G u Pro ggg ttg G y Leu gcg cgg Al a Ar g at c aat Ile Asn 100 at g gat IVet Asp 115 ttt att Phe Il e ttg gtg Leu Val tct atc Ser Ile atc ccc Ile Pro 180 t ca ccg Ser Pro 195 tct gtg Ser Val cat gct Hi s Al a ggt ttg G y Leu cag cag G n n 260 ttg agt Leu Ser 275 gct Al a att SIe gtg Val at c SIe at g let gtg Val gcg Al a gaa G u at g let gat Asp 165 agt Ser caa G n gcg Al a cgt Ar g gtg Val 245 gct Al a ttc Phe Page 240 t tt gt g ggt Phe Val G31y 280 cca ct g cca Pr o Leu Pr o 295 acg gt c ct g Thr Val Leu 310 <210> 190 <211> 313 <21 2> PmT <213> CorynE seqI i st car r ect ed. t xt caa gat t gc t cg acg t gg gat t tg gat gca cct ccc cca O31n As p Cy s Se r Thr Trp Asp Leu Asp Ala Pro Pro Pro 285 290 ccg cta gaa gag t tt t cc ggt cct aaa ggc agg tac gaa Pr o Leu O31u O31u Phe Se r G31y Pr o Lys G31y Ar g Tyr O31u 300 305 cgg Ar g 979 1 027 1 039 ebact er i urn gi ut ani cumn <400> 190 Val O31u 31y Se r Leu Ala Ar g Thr Asp 03 y 03 n A a Phe Leu O31u Pr o As n I I e 03 y Leu Hi s Ala Ala Ar g A a 03 y Il e Asn 1 00 Hi s 03 y I'b t Asp 115 A a 03 u Phe Il e 130 03 u Ser Leu Val 145 Ilie Lys Ser Ilie Leu Asn Ilie Pr o 1 80 G31y Ser Ser Pr o 1 95 Il e Tyr Ser Val 210 Asp Il e Hi s A a 225 Leu Pr o G31y Leu ValI A a I Ie ValI I Ie vb t ValI A a O31u vb t As p 1 65 Ser O31n A a Ar g ValI 245 O31u O31u As n Thr Ser 70 li1e ValI Le u Thr As p 150 Hi s Pr o O31y Tr p A a 230 Th r Ly s Ar g Pr o A a 55 Thr A a Thr Pr o I Ie 135 Ar g As p Pr a Ar g As p 215 Th r Ph e Le u O31y ValI 40 Le u I Ie Ser Ser O31y 120 Thr A a G31y O31n O31u 200 Le u A a ValI G31y Ly s 25 G31y Thr Ser Le u vb t 1 05 Hi s A a G31y (31u G31y 1 85 li1e (31u O31n (31y I Ie As p O31u vb t Thr 75 Hi s As p ValI Tr p Ph e 1 55 Ph e Pr a A a A a Ar g 235 Ser A a As n A a Ph e I Ie A a Ar g As p 140 A a O31n ValI Ly s O31n 220 O31u Tr p ValI O31y Ar g Tr p Ser O31u Tyr 125 Ser As p ValI Le u Tyr 205 As p Pr a Tr p Ph e Pr a A a O31n O31y A a 110 A a Tr p Ser A a Ph e 1 A a Tyr vb t O31u Le u O31y Thr Pr a O31y Ar g Ar g Pr a Ser O31y 1 O31n O31u Ar g Pr a O31u A a Tr p As n Ph e Ar g As n A a A a Le u 1 Pr a A a A a Se r ValI 240 Thr Thr Val Ou Ou Ala Arg 250 255 Page 241 seqi i st car r ect ed. t xt Ala Lys O31n O31n Ala Leu Asn Ala Leu Leu Pro Val Lys Asp Ser Leu 260 265 270 Asn O31n Leu Ser Phe Phe Val O31y O31n Asp Cys Ser Thr Trp Asp Leu 275 280 285 Asp Ala Pr o Pr o Pro Pro Leu Pr o Pr o Leu O31u O31u Phe Se r G31y Pro 290 295 300 Lys O31y Ar g Tyr O31u Th r Val Leu Ar g 305 310 <210> 191 <211> 924 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221> CDS <222> (901) <223> RXN00905 <400> 191 cgct gcccct ct at gct gct cct agt t acc cct gcacaaa t agcggt t t t t ct cacgcat t ct gcat cga gt cgggt cga cgt at at aag gt ggaaaggc at g acc caa t tc gaa l'bt Thr 03 n Phe 03 u aac As n tt t Ph e cca Pr o ac a Thr gga G31y aag Ly s g at As p c ac Hi s ct g Le u ac c Thr c aa O31n c ag O31n ct a Le u ac c Thr ac c Thr ct g Le u t gg Tr p ac c Thr 120 at g vb t Ct C Le u ct t Le u aaa Ly s gag O31u gca A a tt c Ph e t ac Tyr ac c Thr t gg Tr p ct c Le u Ct C Le u aaa Ly s gaa O31u gac As p ggt O31y at c I Ie 75 ag c Ser aat As n t ac Tyr gca A a acc Thr gag O31u at t I Ie t ac Tyr ct c Le u ggC O31y c ac Hi s cct Pr o ggC O31y gca A a 140 t ac Tyr at c I Ie t ct Ser ggC O31y gt c ValI cgc Ar g g ac As p g aa O31u 110 ggC O31y cgc Ar g gt c ValI g aa O31u 15 tt c Ph e gcg A a ag c Ser aag Ly s gt t ValI 95 ct c Le u gcc A a gcc A a aac As n aac As n aaa Ly s g aa O31u O31y gt c ValI ac c Thr gct A a gt c ValI 1 C aa O31n t cc Ser g aa O31u Ph e t cc Ser cca Pr o gag O31u g ac As p g aa O31u 145 cgc Ar g gt g ValI t gg Tr p gct A a g aa O31u t cc Ser cgc Ar g t gc Cys 130 gcc A a gag O31u C ac Hi s gt a ValI gcc A a ggt O31y ggC O31y g ac As p 115 aag Ly s ag c Ser C ag O31n ag c Ser aaa Ly s g aa O31u gca A a cct Pr o 1 00 gct A a ggC O31y ggc O31y gag ggc t cc gag gaa at g gga O31u O31y Ser O31u O31u [vbt O31y Page 242 INO seql i st cor r ect ed. t xt 150 155 160 165 Sggc gga gcg ctc agc gcg ctc atc aag gac aag cct gag ctt ttc gac 643 Gy y Ala Leu Ser Al a Leu Ile Lys Asp Lys Pro G u Leu Phe Asp 170 175 180 4 gca gat gtc atc ttg att gca gac agc gga aac gct tcc gtg ggc acc 691 Ala Asp Val lie Leu Ile Ala Asp Ser Gy Asn Ala Ser Val G y Thr 185 190 195 cca acc ttg acc act acc ctg cgc ggt ggc gga cag gtc acc gtc acc 739 Pro Thr Leu Thr Thr Thr Leu Arg Gy Gy Gy G n Val Thr Val Thr C\ 200 205 210 00 gtg gac acc ctt gaa ggc gct gtt cac tcc ggc cag aac ggt ggc gct 787 0 Val Asp Thr Leu G u G y Al a Val Hi s Ser G y G n Asn G y G y Al a 215 220 225 (N gcc cca gat gct gtt gct gct ctc gtg cgc gtt ctg gat act ttg cgc 835 0 Ala Pro Asp Ala Val Ala Ala Leu Val Arg Val Leu Asp Thr Leu Arg S230 235 240 245 (N gat gaa cac gga cgc acc gtt atc gac ggc tgt caa cac cac cgc aaa 883 Asp G u His G y Arg Thr Val lie Asp G y Cys G n His His Arg Lys 250 255 260 ctg gaa ggg cga gcc tta tgatccagag actttccgca gcg 924 Leu G u G y Arg Al a Leu 265 <210> 192 <211> 267 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 192 IVet Thr n Phe u Asn Al a n Val Leu Lys u Asn I e u Asn 1 5 10 On Arg u On lie Phe Thr n Leu Lys u Ile Val Ser Phe Asn 25 Ser Val His Ser Asp Pro Asn Leu Leu G u Asp Tyr Ala G y Ala Lys 40 G u Trp Val Lys G u Thr Leu Thr Asn Ala y Leu Thr Val Ser G u 55 Phe Ala Ala G u Asp G y Thr Thr Asn Phe I e y Thr Arg Lys y 70 75 Ser G u y Ala Pro Lys Val Leu Leu Tyr Ser His Phe Asp Val Val 90 Pro Ser y Pro Leu Asp Leu Trp Asp Thr Asn Pro Phe G u Leu Thr 100 105 110 G u Arg Asp Ala G y His G y Thr Arg Trp Tyr G y Arg G y Ala Ala 115 120 125 Asp Cys Lys G y Asn Leu Val Iet His Leu Ala Ala Leu Arg Ala Val 130 135 140 G u Ala Ser y Asp Thr Thr Leu Asn Leu Thr Tyr Val Val G u y 145 150 155 160 Ser u u IVet y y y Ala Leu Ser Ala Leu Ile Lys Asp Lys Page 243 INO seql i st cor r ect ed. t xt 165 170 175 Pro G u Leu Phe Asp Al a Asp Val lie Leu lie Ala Asp Ser y Asn 180 185 190 Ala Ser Val y Thr Pro Thr Leu Thr Thr Thr Leu Arg Gy Gy y S195 200 205 G n Val Thr Val Thr Val Asp Thr Leu G u G y Ala Val His Ser G y 210 215 220 G n Asn Gy y Ala Al a Pro Asp Ala Val Ala Ala Leu Val Arg Val C 225 230 235 240 00 Leu Asp Thr Leu Arg Asp G u His G y Arg Thr Val Ile Asp G y Cys 245 250 255 C G n His His Arg Lys Leu G u G y Arg Ala Leu 260 265 rC <210> 193 <211> 716 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> FRXA00905 <400> 193 gat cca aac cta ctg gag gac tac gcc ggc gcg aaa gaa tgg gta aaa 48 Asp Pro Asn Leu Leu G u Asp Tyr Ala Gy Ala Lys u Trp Val Lys 1 5 10 gaa aca ctg acc aac gca ggt ctc acc gtc agc gaa ttc gct gcc gaa 96 G u Thr Leu Thr Asn Al a y Leu Thr Val Ser G u Phe Ala Ala G u 25 gat gga acc acc aac ttc atc ggc acc cgc aag ggc tcc gaa ggt gca 144 Asp y Thr Thr Asn Phe Ile y Thr Arg Lys y Ser u y Ala 40 cca aag gta ctg ctg tac agc cac ttc gac gtt gtc cca tcc ggc cct 192 Pro Lys Val Leu Leu Tyr Ser His Phe Asp Val Val Pro Ser y Pro 55 ttg gat ctc tgg gac acc aat cct ttt gaa ctc acc gag cgc gac gct 240 Leu Asp Leu Trp Asp Thr Asn Pro Phe G u Leu Thr G u Arg Asp Ala 70 75 ggc cac ggc acc cgc tgg tac ggc cgc ggc gcc gct gac tgc aag ggc 288 G y His G y Thr Arg Trp Tyr G y Arg G y Ala Ala Asp Cys Lys G y 90 aac ctg gtc atg cac ctc gca gca ctg cgc gcc gtc gaa gcc agc ggc 336 Asn Leu Val IVet His Leu Ala Ala Leu Arg Ala Val G u Ala Ser G y 100 105 110 gac acc aca ctc aac ctc acc tac gtg gtc gag ggc tcc gag gaa atg 384 Asp Thr Thr Leu Asn Leu Thr Tyr Val Val G u y Ser G u G u Iet 115 120 125 gga ggc gga gcg ctc agc gcg ctc atc aag gac aag cct gag ctt ttc 432 Gy Gy Gy Ala Leu Ser Ala Leu Ile Lys Asp Lys Pro G u Leu Phe 130 135 140 Page 244 gac gca gat gtc atc ttg att Asp Ala Asp Val lie Leu Ile 145 150 acc cca acc ttg acc act acc Thr Pro Thr Leu Thr Thr Thr 165 acc gtg gac acc ctt gaa ggc Thr Val Asp Thr Leu G u y 180 gct gcc cca gat gct gtt gct Ala Ala Pro Asp Ala Val Ala 195 cgc gat gaa cac gga cgc acc Arg Asp G u His G y Arg Thr 210 215 aaa ctg gaa ggg cga gcc tta Lys Leu G u Gy Arg Al a Leu 225 230 <210> 194 <211> 231 <212> PRT <213> Cor ynebact er i um gl ut ar <400> 194 Asp Pro Asn Leu Leu G u Asp 1 5 G u Thr Leu Thr Asn Al a y Asp G y Thr Thr Asn Phe Ile Pro Lys Val Leu Leu Tyr Ser Leu Asp Leu Trp Asp Thr Asn 70 G y His G y Thr Arg Trp Tyr Asn Leu Val IVet His Leu Ala 100 Asp Thr Thr Leu Asn Leu Thr 115 G y G y G y Al a Leu Ser Al a 130 135 Asp Ala Asp Val lie Leu Ile 145 150 Thr Pro Thr Leu Thr Thr Thr 165 Thr Val Asp Thr Leu G u y 180 seql i st cor r ect ed. t xt gca gac agc gga aac gct tcc Ala Asp Ser G y Asn Al a Ser 155 ctg cgc ggt ggc gga cag gtc Leu Arg G y G y G y G n Val 170 gct gtt cac tcc ggc cag aac Ala Val His Ser G y G n Asn 185 190 gct ctc gtg cgc gtt ctg gat Ala Leu Val Arg Val Leu Asp 200 205 gtt atc gac ggc tgt caa cac Val lie Asp G y Cys G n Hi s 220 tgatccagag actttccgca gcg gtg Val acc Thr 175 ggt G y act Thr cac Hi s Al a Thr 25 Thr Phe Phe Ar g Leu 105 Val l Ie Asp Ar g Val 185 Al a Ser Lys Val Leu Al a Al a G u Asp Sy 155 Sy Ser G u Phe Ser Pr o G u Asp G u Ser 125 Pr o Al a G n G n Tr p Al a G u Ser Ar g Cys Al a 110 G u G u Ser Val Asn 190 Val Al a Sy Sy Asp Lys Ser G u Leu Val Thr 175 Sy Ala Ala Pro Asp Ala Val Ala Ala Leu Val Arg Val Leu Asp Thr Leu Page 245 seql i st cor r ect ed. t xt 195 200 205 Arg Asp G u His y Arg Thr Val lie Asp Gy Cys On His His Arg 210 215 220 Lys Leu G u G y Arg Al a Leu 225 230 <210> 195 <211> 627 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(604) <223> RXA00906 <400> 195 accgtggaca cccttgaagg cgctgttcac tccggccaga acggtggcgc tgccccagat gctgttgctg ctctcgtgcg cgttctggat actttgcgcg atg aac acg gac gca IVet Asn Thr Asp Ala ccg Pr o t at Tyr gac Asp cct Pr o ct c Leu cca Pr o ct g Leu gac Asp acc Thr acc Thr 150 tcg Ser cca Pr o at g Met at c SIle gca Al a ggc Gy aat Asn aac Asn 120 gcg Al a ggc Gy gct Al a act Thr gac Asp at c SIle ccc Pr o gaa G u aca Thr ccg Pr o tgc Cys ggc Gy gtc aac Val Asn ttc cgc Phe Arg ggc gac Sy Asp acc gga Thr y gca acg Al a Thr 75 gca aac Al a Asn cct tgg Pro Trp ttc tcc Phe Ser ctg agc Leu Ser 140 gga tcc G y Ser 155 acc acc gca Thr Thr Al a 15 agc gat gcc Ser Asp Al a aac cca gca Asn Pr o Al a 45 ttc act tcc Phe Thr Ser gcg tcc gcc Al a Ser Al a gat gtg gcc Asp Val Al a 95 ggc gca aag G y Al a Lys 110 acc gat att Thr Asp Ile 125 gct gcg tac Al a Al a Tyr at t cca ct g Ile Pro Leu aac Asn ggc Gy agc Ser acc Thr aag Lys 80 gag G u at c SIle acc Thr gag G u tgt Cys 160 1 tgg Tr p at c SIle at g Met cca Pr o ct a Leu aag Lys acg Thr ggc Gy ggc Gy 145 ace Thr aag Lys ct c Leu ct g Leu gt g Val aac Asn ct g Leu gt g Val cct Pr o 130 aag Lys gaa G u gag Gu ggt Gy t cc Ser gaa G u cgc Ar g cag G n 100 at c SIle at g Met ctt Leu at t SIle cct Pr o gta Val agg Ar g gca Al a gtg Val cac Hi s gat Asp t cc Ser gtc Val gag G u 165 gtc aac cca Val Asn Pro t aagcagaat t ggcact ct a cgg Page 246 seql i st cor r ect ed. t xt <210> 196 <211> 168 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 196 IVet Asn Thr 1 Trp Lys G y Ile Leu Asp IVet Leu Tr p Pro Val Al a Leu Asn Leu Lys Leu Lys Thr Val G u 115 G y Pro Al a 130 G y Lys Asp 145 Thr G u Leu Asp Al a 5 G u Pro G y Val Ser Arg G u Al a Arg Val G n Hi s 100 Ile Asp Met Ser Leu Val Ile G u 165 Pro Leu Ser Thr Asp SIle Al a Asn Al a SIle SIle Leu 135 a u Asn Pr o Met 40 l le Al a Sy Asn Asn 120 Al a G y Pr o G u 25 Sy Ser Val Leu Hi s 105 G n Ser Ser Al a 10 Thr Asp SIle Pr o G u 90 Thr Pr o Cys Sy Val Phe Sy Thr Al a Al a Pr o Phe Leu Sy 155 Thr Ser Asn Phe Al a Asp Sy Thr 125 Al a SIle Thr Asp Pr o Thr Ser Val Al a 110 Asp Al a Pr o Al a Al a Al a Ser Al a Al a Lys SIe Tyr Leu <210> 197 <211> 246 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(223) <223> RXA00907 <400> 197 cctgagcgct gcgtacgagg gcaaggatct tgtcaccgaa ggcagcggcg gatccattcc actgtgtacc gaactgattg aggtcaaccc ataagcagaa ttg gca ctc tac ggt Leu Ala Leu Tyr G y 1 gtg gaa gaa ccc ctc acc gtt atc cac tcc gct aat gaa tct gtt gac Val G u u Pro Leu Thr Val Ile His Ser Ala Asn u Ser Val Asp 15 ccc aat gag att cgc gat atc gcc acc gca gaa gca ttg ttc ctg ctc Pro Asn Gu lie Arg Asp lie Ala Thr Ala u Ala Leu Phe Leu Leu 30 Page 247 seql i st cor r ect ed. t xt aac tac acc aag tagacccaaa agcaggcgtt aac Asn Tyr Thr Lys <210> 198 <211> 41 <212> PRT <213> Corynebacter i um gl utam cum <400> 198 Leu Ala Leu Tyr y Val G u G u Pro Leu 1 5 10 Asn Gu Ser Val Asp Pro Asn G u ie Arg 25 Ala Leu Phe Leu Leu Asn Tyr Thr Lys Thr Val Ile His Ser Al a Asp l e Al a Thr Al a G u <210> <211> <212> <213> <220> <221> <222> <223> 199 1386 DNA Cor ynebact er i um CDS (101)..(1363) RXA02101 gl ut am cum <400> 199 gccatggaat gctccgt t ga acgcaacagc cttaaataca atcccctcct ataagccaag agttttagtg tcgctgcgca ggtactctac tatctaatcc agc cgc att Ser Arg Ile gaa ctt cta aac G u Leu Leu Asn aat Asn cat ggt gtt gat His y Val Asp tcg tgg caa gag Ser Trp n u gcc gca Al a Al a t at cag gat Tyr G n Asp acc gca gat Thr Al a Asp cac gaa cat cct His G u His Pro gag 3u ctc tcc ggc ttc Leu Ser G y Phe gaa tca gag Su Ser G u tgt gag gtg Cys G u Val cgc att cag aaa Arg lie n Lys ctc gag cgt ttt Leu G u Arg Phe gat Asp att cca Ile Pro aat gtt ggc ggt Asn Val y G y t ac Tyr ggc att ctg gcc Sy Ile Leu Al a ttc cga aat ggg Phe Arg Asn y tcg Ser aca gat cct ggt Thr Asp Pro Gy gcc Al a cct gtt gcg tta Pro Val Ala Leu cgc gca gat ttc Arg Ala Asp Phe ggc ctt ccc gtc Sy Leu Pro Val aag Lys gaa atc acc gga G u I e Thr G y ccg ttt gct tcc Pro Phe Ala Ser act cgt Thr Arg 100 atg cgt ccg IVet Arg Pro gat ggg gca aat Asp y Ala Asn gt c Val 110 cat gtc atg cac His Val Met His gca tgc ggc Al a Cys G y 115 cac gat gtc cac gtc acc gcg ctg ctt His Asp Val His Val Thr Ala Leu Leu ggt gcg tgt gcc att tta gat Sy Ala Cys Ala lle Leu Asp Page 248 seql i st cor r ect ed. t xt 130 ttg ttc cag cca Leu Phe G n Pro gag cgt G u Arg 135 cgc gat gca tgg Arg Asp Ala Trp gaa 10u 140 ggc acg ttc atc Sy Thr Phe Ile tcg Ser 150 gag gaa aac t cc G u G u Asn Ser caa G n 155 ggc gct aac aag G y Al a Asn Lys at g Met 160 gtc gcc ggc ggt Val Al a Gy y gtt gat ctg atc Val Asp Leu Ile cca Pr o 170 cgc cct gat gtg Arg Pro Asp Val ttt ggc cag cat Phe G y G n Hi s gta gtc Val Val 180 ccc ggt gct Pr o G y Al a gcc tgc gat Al a Cys Asp 200 gga acc gtg atg Sy Thr Val Mbet agc Ser 190 atg cct ggc ggt bet Pro Gy y gct t c gct Al a Leu Al a 195 gcc cat ggt Al a Hi s G y tcc att gaa atc Ser Ile G u lle att cag ggt cgc lie n y Arg agc Ser 210 t cc at g Ser 'bet 215 cct cat aat t cc Pro His Asn Ser at c Ile 220 gat ccc act tat Asp Pro Thr Tyr gca gcg atg att Ala Ala IVet Ile gt c Val 230 gtg cga ctc caa Val Arg Leu G n gga G3y 235 atc gtg ggc cgc Ile Val Gy Arg gag gtt G u Val 240 t ct cca gag Ser Pro G u ttc gcc gtt att Phe Al a Val I le tct Ser 250 gtg ggc acc ct c Val G y Thr Leu tcg ggc aac acc Ser G y Asn Thr aac aac Asn Asn 260 acc att cca Thr Ile Pro gac aaa gt c Asp Lys Val 280 agt gct cgt ttg Ser Al a Arg Leu gt g Val 270 ttg aac tgc cgt Leu Asn Cys Arg ttc tac aac Phe Tyr Asn 275 gtt gtc cgt Val Val Arg aag cac aag gtc Lys His Lys Val cga gcc atc gaa Arg Ala lie u cgt Ar g 290 ggt gaa y295 295 tgc ctt gct tcc Cys Leu Al a Ser ggt G3y 300 att gag gaa gaa lie u G u u gtc att gag tac Val Ile G u Tyr ttc Phe 310 gcc cac ggt gat Al a Hi s G y Asp acc aac aac acc Thr Asn Asn Thr cct Pr o 320 gtt gtc ttc gat Val Val Phe Asp gtg cgc cct gtc Val Arg Pro Val ttc Phe 330 gac gat gtt ttc Asp Asp Val Phe gag gat tct att Su Asp Ser Ile gac gct Asp Al a 340 tac cgg tgg Tyr Arg Tr p aac agc cct Asn Ser Pro 360 gcg tcg gag gat Ala Ser G u Asp ttc Phe 350 ccc tcc att cct Pro Ser Ile Pro aag gca ttc Lys Ala Phe 355 cgc gat cag Arg Asp G n 1027 1075 1123 1171 1219 1267 1315 tac ctg tac tgg Tyr Leu Tyr Trp att ggt gtc acg lie y Val Thr ccg Pr o 370 tgg aca Trp Thr 375 gaa gcc gta gaa Su Ala Val u aga Ar g 380 gac cgc gtg gca Asp Arg Val Ala gat gtg cca gcc Asp Val Pro Ala aat cac atg gga gat ttc ctc cct gat Asn His Vet G y Asp Phe Leu Pro Asp tat gcg ccg acg atg tcc gct Tyr Ala Pro Thr IVet Ser Ala Page 249 seqI i st car r ect ed. t xt 390 395 400 405 gcc acc cgc gca gcc gca gcc gcg ctg ctg acc tac ttg gga act aac Al a Th r Ar g Al a Al a Al a Ala Ala Leu Leu Thr Tyr Leu G31y Thr Asn 410 415 420 t aat cat ct a gtt t tct gcg acg <210> 200 <211> 421 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn 1363 1386 <400> 200 I'b t Se r Ar g I Ie Trp O31n O31u Al a G3 y Phe G3 u Ser Phe Asp Cys G3 u Val Phe Arg Asn Ar g Al a Asp Phe Phe Al a Ser Thr 1 00 I'b t Hi s Al a Cys 115 Cys Al a Il e Leu 130 Al a Leu Phe 03 n 145 Val Al a 31y G31y 03 y 03 n Hi s Val 1 80 03 y 03 y Al a Leu 1 95 Ar g Ser Al a Hi s 210 Val Al a Al a I'b t 225 Val Ser Pr o O31u G31y Asn Thr Asn 260 Cys Ar g Phe Tyr 275 Ser Al a O31u ValI G31y As p Ar g G31y As p Pr o Le u 1 65 ValI Al a Gy le As p 245 As n As n 03 u Leu Leu Asn O31n Al a Pr o 55 Thr Le u Ar g As p Ar g 135 O31u As p G31y Cys vb t 215 ValI Al a I Ie Lys5 Ph e Ar g ValI Pr o ValI Hi s 1 Hi s As p As n I Ie Al a 1 Ser Hi s Le u I Ie Al a 265 Lys5 Hi s O31u O31n G31y Al a 75 (31u G31y Thr Tr p O31n 1 55 Ar g Thr O31u Ser O31y 235 ValI Al a O31y Hi s Lys5 Tyr Pr o I Ie Al a Al a O31u 140 O31y Pr a ValI I Ie I Ie 220 li1e O31y Ar g ValI Pr a Tyr O31y ValI Thr As n Le u 125 O31y Al a As p vb t Ar g 205 As p ValI Thr Le u Tyr 285 Le u Le u O31u Le u Le u ValI Hi s O31y Ph e Lys5 Cys 1 vb t O31n Thr Ar g O31n 255 Le u Ser Ser Ar g Al a vb t Pr a ValI Al a I Ie vb t 1 Ph e Pr a O31y Tyr O31u 240 Ser As n Hi s Lys Val Ar g Al a I Ie Page 250 G u Pr o 305 Val Asp SIle Thr Ser 385 Pr o Tyr Val SIle Phe SIle Lys 355 Ar g Val bet Gy Ar g Tyr Thr 325 Al a Phe G n Al a Al a 405 Asn G u 295 Al a Ar g Ar g Ser Thr 375 Hi s Thr seql i st cor r ect ed. t xt Cys Leu Al a Ser G y II e 300 Hi s G y Asp Leu Thr Asn 315 Pro Val Phe Asp Asp Val 330 Trp Thr Ala Ser G u Asp 345 Pro Tyr Leu Tyr Trp Thr 360 365 G u Ala Val G u Arg Asp 380 IVet y Asp Phe Leu Pro 395 Arg Ala Ala Ala Ala Ala 410 G u Thr Gy 335 Pr o Gy Val Tyr Leu 415 G u Pr o 320 G u Ser Val Al a Al a 400 Thr <210> 201 <211> 1389 <212> DNA <213> Corynebacteri um gl ut am cum <220> <221> CDS <222> (101)..(1366) <223> RXN02565 <400> 201 ggaaattcga tacagtgcga tgacgcgata ttagaaagaa aaccctcacc ctccttcagg aacttatccg caacgcctgc cca Pr o ttt Phe ggc Gy gag G u cct Pr o at t l Ie t ca Ser gga Gy acc Thr tta Leu tgg Tr p ggt Gy cag G n ccc Pr o at t SIle ct g Leu aaa Lys ggg Gy gaa G u aac Asn at c SIle ctt Leu gat Asp 75 t cc Ser at t SIle gtt Val gt g Val gga Gy cca Pr o gt c Val aga Ar g aaa Lys act Thr 45 cat Hi s ttc Phe gat Asp aac Asn at c SIle gtt Val act Thr ggt Gy at g Met gcg gaa Al a u 15 acc aag Thr Lys cca ggc Pro G y gat gtt Asp Val gcg gag Al a G u 80 ctc ttt Leu Phe Page 251 aaagatgcgc tttacgacga gtg aat gat cta acc Val Asn Asp Leu Thr 1 agc cta gaa cgt ttc Ser Leu G u Arg Phe ctg gaa ccg cat ccg Leu G u Pro His Pro agc gat cca gat gct Ser Asp Pro Asp Ala gtg cct gtt gat ctg Val Pro Val Asp Leu att tcg gat gga cag I e Ser Asp G y G n att acc gca acc caa Ile Thr Ala Thr G n NO seql i st cor r ect ed. t xt 90 95 100 gcg gcc gtc acc cgt caa gta gcc cgt gaa ggc ggc ctg cgt ggc acg 451 Ala Ala Val Thr Arg n Val Ala Arg u G y G y Leu Arg G y Thr S105 110 115 4 ctg aca ttc gtt ggc gtt gct gat gag gaa gcc cgc ggc gga ctc gga 499 Leu Thr Phe Val y Val Ala Asp u u Ala Arg y y Leu y 120 125 130 gcg aag tgg ctt tcc gaa gaa cac caa aac ctc ttc agc tgg aaa aac 547 Ala Lys Trp Leu Ser u u His n Asn Leu Phe Ser Trp Lys Asn K1 135 140 145 00 tgc ctc tcc gaa tcc ggt gga tcg cac ctt cca gtc cac gac ggc agc 595 SCys Leu Ser u Ser O y O y Ser His Leu Pro Val His Asp O y Ser S150 155 160 165 .C gac gca gta gta att aac gtt gga gaa aaa ggt gca gct caa cgt cgt 643 SAsp Ala Val Val Ile Asn Val y u Lys y Ala Ala n Arg Arg 170 175 180 att cac gtc aat ggc gat gct ggt cat ggt tcc att cct ttc gac cgt 691 Ile His Val Asn O y Asp Ala O y His O y Ser Ile Pro Phe Asp Arg 185 190 195 gac agc gct att gtc aag at c ggt gaa gtc gcc cgc cga at c gct gcc 739 Asp Ser Ala Ile Val Lys Ile G y G u Val Ala Arg Arg Ile Ala Al a 200 205 210 gcc gat ctg aag gta gcc aag gac gat atc tgg caa ggc ttc gtc caa 787 Ala Asp Leu Lys Val Ala Lys Asp Asp Ile Trp n y Phe Val n 215 220 225 gcg cac cgt ttc gac cca gaa acg gag cag gcg ctt ctt agc ggg acc 835 Ala His Arg Phe Asp Pro O u Thr O u O n Ala Leu Leu Ser O y Thr 230 235 240 245 tcc cct gag gcc tac gca gag ttc ggc gga ctc tcc cgc ttc gcc cac 883 Ser Pro O u Ala Tyr Ala O u Phe O y O y Leu Ser Arg Phe Ala His 250 255 260 gcg gtg tct cat ctc acg atc gcc caa act gtg gtt cgt gca ggt caa 931 Ala Val Ser His Leu Thr Ile Ala O n Thr Val Val Arg Ala O y O n 265 270 275 gcc atc aat gta ttg cca tcg cat gcg tac ttg gaa ctg gat atc cgt 979 Ala Ile Asn Val Leu Pro Ser His Ala Tyr Leu u Leu Asp Ile Arg 280 285 290 acc ctt cca ggc caa acc aat gac tat gtt gat gac acc ctg cgt gct 1027 Thr Leu Pro y n Thr Asn Asp Tyr Val Asp Asp Thr Leu Arg Ala 295 300 305 gct ctg ggc gat ctt gcc gat gaa gta gaa atc gaa cac ctc atc tct 1075 Ala Leu 3 y Asp Leu Ala Asp 3 u Val O u Ile 3 u His Leu Ile Ser 310 315 320 325 gaa gaa gca acg gtg agc cca act gat tcc agg ttg tat aac acc ttg 1123 Su u Ala Thr Val Ser Pro Thr Asp Ser Arg Leu Tyr Asn Thr Leu 330 335 340 gaa aaa gtt ctt ggt gat ttc ttc ccc gat gcg cct gtg gtc cca att 1171 Su Lys Val Leu y Asp Phe Phe Pro Asp Ala Pro Val Val Pro Ile 345 350 355 att tcc tct ggt ggc tct gac ctg cgc ttt ggt cgt cga cta ggc ggt 1219 Ile Ser Ser y y Ser Asp Leu Arg Phe y Arg Arg Leu y y Page 252 gt t ValI gca Al a 390 ct t Le u ggc G31y seqi i st car r ect ed. t xt 360 365 370 ggt t at ggt ttt gca gt t cat gca cgt gaa cga act ttg gcg gaa O31y Tyr O31y Phe Al a Val Hi s Al a Ar g O31u Ar g Thr Leu Ala O31u 375 380 385 at g ggg caa ct t cac t cc cat gac gag gcg ct g t ac ct g gaa gat 'b t G31y O31n Leu Hi s Ser Hi s Asp O31u Al a Leu Tyr Leu O31u Asp 395 400 405 gaa ct g act gt t cgg ggt t at gac t cc gt c gt g cgt gaa t tc ct a O31u Leu Thr Val Ar g G31y Tyr Asp Ser Val Val Ar g O31u Phe Leu 410 415 420 t aaaaacat g aagcaggagt ct t 1267 1315 1363 1389 <210O> 202 <21 1> 422 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 202 Val Asn A S e r L e u C 3e 3 Ser As p P Val Pr o V 1 1 e Ser A I I e Thr A 03 y Leu A Ar g G31y C33 Phe Ser T 145 Val Hi s A A l a A l a C 3 P Ar g Ar g 1 210 sp Leu Iu Ar g 20 ro Hi s r o As p aI As p sp 03 y I a Thr 1 00 r g G31y y Leu r p Lys sp 03 y In Ar g 1 80 he As p 1 e Al a Thr Ph e Pr o Al a Le u O31n O31n Thr G31y As n Ser 1 65 Ar g Ar g Al a Pr o Asp Se r G y Ph e G31y (31u Pr o 70 li1e Al a Le u Al a Cy s 150 As p I Ie As p Al a (31u Ar g Pr o 55 Lys5 Tr p Al a Thr Lys5 135 Le u Al a Hi s Ser As p 215 G31y Thr 40 Le u Tr p G31y ValI Ph e 120 Tr p Ser ValI ValI Al a 200 Le u Thr Ser Thr Thr Ar g Thr 1 ValI Le u (31u ValI As n 1 li1e Lys5 G u As n I Ie Le u As p Ser O3n ValI Ou Oy 1 As n As p Lys5 Al a I Ie ValI ValI O31y Pr o ValI ValI Al a O31u 140 O31y ValI Al a I Ie Lys5 220 Ar g Ly s Thr Hi s Ph e As p Al a As p 125 Hi s Ser O31y O31y O31y 205 As p Al a Thr Pr a As p Al a Le u O31u O31u As n Le u Ly s 1 O31y ValI I Ie On Oy Phe Val On Ala His Arg Phe Asp Pro Ou Thr Ou On Ala Page 253 seql i st cor r ect ed. t xt 225 235 Al a G u Phe G y Leu Leu Ser Thr Ser Pro G u Ala Ser Val G u Asp 305 G u Leu Pr o Ar g Ar g 385 Leu Val Phe Al a 275 Asp Leu Leu Asn Val 355 Leu Leu Leu G u 245 Hi s G n Ar g Al a Ser 325 Leu SIle Gy G u Asp 405 Leu Al a Al a Thr Al a 310 G u G u SIle Val Al a 390 Leu Gy Val l Ie Leu 295 Leu G u Lys Ser Gy 375 b t Gu Hi s 265 Val Gy Asp Thr Leu 345 Gy Gy G n Thr Thr Pr o Thr Al a 315 Ser Asp Ser Al a Hi s 395 Ar g Al a Hi s 285 Asp G u Thr Phe Leu 365 Hi s Hi s Tyr n 270 Al a Tyr Val Asp Pr o 350 Ar g Al a Asp Asp 240 G y Leu 255 Thr Val Tyr Leu Val Asp Su lle 320 Ser Arg 335 Asp Al a Phe G y Arg G u G u Al a 400 Ser Val 415 <210> 203 <211> 365 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> FRXA02565 <400> 203 gct gct ctg ggc gat ctt gcc gat gaa Al a Ala Leu G y Asp Leu Al a Asp G u 1 5 tct gaa gaa gca acg gtg agc cca act Ser G u G u Ala Thr Val Ser Pro Thr 25 ttg gaa aaa gtt ctt ggt gat ttc ttc Leu G u Lys Val Leu G y Asp Phe Phe 40 att att tcc tct ggt ggc tct gac ctg Ile Ile Ser Ser G y G y Ser Asp Leu 55 ggt gtt ggt tat ggt ttt gca gtt cat G y Val G y Tyr G y Phe Ala Val Hi s gt a gaa at c Val Gu Ile 10 gat tcc agg Asp Ser Arg ccc gat gcg Pro Asp Al a cgc ttt ggt Arg Phe G y gca cgt gaa Al a Ar g G u Page 254 gaa G u ttg Leu cct Pr o cgt Ar g cga Ar g cac Hi s t at Tyr gt g Val cga Ar g act Thr ct c Leu aac Asn gt c Val ct a Leu ttg Leu at c SIe acc Thr cca Pr o ggc Gy gcg Al a INO seqI i st car r ect ed. t xt 70 75 ci gaa gca atg ggg caa ctt cac tcc cat gac gag gcgct g tac ct ggaa 288 O31u Al a I'b t 31y O31n Leu Hi s Se r Hi s As p G31u Ala Leu Tyr Leu G31u 90 Sgat ct t gaa ct g act gt t cgg ggt t at gac t cc gt c gt g cgt gaa t tc 336 Asp Leu G31u Leu Thr Val Ar g G31y Tyr Asp Ser Val Val Ar g G31u Phe cI100 105 110 ct a ggc t aaaaacat g aagcaggagt ct t 365 Leu 03 y 00 S<210> 204 S<211> 114 ci <212> PRT .C <213> Cor ynebact er i urn gi ut anm curn S<400> 204 ciAl aAl aLeu OyAsp LeuAlaAsp(3uVal Gu lle(3u His Leu Ilie 1 5 10 Ser G31u G31u Al a Th r Val Ser Pr o Thr Asp Ser Ar g Leu Tyr Asn Thr 25 Leu G31u Lys Val Leu G31y As p Phe Phe Pr o Asp Al a Pr o ValI Val Pr o 40 Ilie I Ie Ser Ser G31y G31y Se r Asp Leu Ar g Ph e G31y Ar g Ar g Leu G31y 55 G31y Val G31y Tyr G31y Phe Ala Val Hi s Al a Ar g (31u Ar g Th r Leu Ala 70 75 O31u Al a 'b t G31y O31n Leu Hi s Ser Hi s Asp O31u Ala Leu Tyr Leu O31u 90 Asp Leu O31u Leu Thr Val Ar g G31y Tyr Asp Ser Val Val Ar g O31u Phe 100 105 110 Leu 03 y <210> 205 <211> 738 <212> DNA <213> Cor ynebact er i urn gi ut anm curn <220> <221> OIDS <222> (738) <223> FRXA02567 <400> 205 ct t at ccgc aac gcc tgc gtg aat gat ct aacc cca gat t ca ggt cag 48 Leu IlIe Ar gAs nAl aCys Val Asn Asp Leu Thr Pr oAsp Ser O31y (1n 1 5 10 gaa at t aga aac gcg gaa agc ct a gaa cgt t tc t tt gaa gga acc ccc 96 (31u Ilie Ar g Asn Al a (31u Se r Leu (31u Ar g Phe Phe (31u G31y Thr Pr o 25 aac gtt aaa atc acc aag ctg gaa ccg cat ccg ggc cgg acc tca att 144 Asn Val Lys Ilie Thr Lys Leu (3u Pr o Hi s Pr o (3y Ar g Thr Ser Ilie 40 Page 255 seql i st cor r ect ed. t xt at c SIle ctt Leu gat Asp t cc Ser caa G n gtt Val gaa G u 145 ggt G y aac Asn gat Asp aag Lys gcc Al a 225 cca Pr o act Thr cat Hi s ttc Phe gat Asp gcc Al a 115 gat Asp cac Hi s tcg Ser gga G y ggt G y 195 ggt G y gac Asp acg Thr cca Pr o gat Asp gcg Al a ct c Leu gaa G u gaa G u aac Asn ctt Leu 165 aaa Lys ggt Gy gt c Val at c SIle cag G n 245 ggc Gy gtt Val 70 gag G u ttt Phe ggc G y gcc Al a ct c Leu 150 cca Pr o ggt Gy t cc Ser gcc Al a tgg Tr p 230 gcg Al a agc gat cca gat gct gag Ser Asp Pro Asp Ala G u gtg cct gtt gat ctg cct Val Pro Val Asp Leu Pro 75 att tcg gat gga cag att Ile Ser Asp G y G n lie 90 att acc gca acc caa gcg Ile Thr Ala Thr G n Ala 105 ggc ctg cgt ggc acg ctg Gy Leu Arg Gy Thr Leu 120 cgc ggc gga ctc gga gcg Arg G y G y Leu Gy Ala 135 140 ttc agc tgg aaa aac tgc Phe Ser Trp Lys Asn Cys 155 gtc cac gac ggc agc gac Val His Asp G y Ser Asp 170 gca gct caa cgt cgt at t Ala Ala n Arg Arg lie 185 att cct ttc gac cgt gac lie Pro Phe Asp Arg Asp 200 cgc cga atc gct gcc gcc Arg Arg lie Ala Ala Ala 215 220 caa ggc ttc gtc caa gcg Sn Gy Phe Val n Ala 235 cct tta aca Pro Leu aaa t gg Lys Tr p tgg ggt Trp G y gcc gtc Al a Val 110 aca ttc Thr Phe 125 aag tgg Lys Tr p ctc t cc Leu Ser gca gt a Al a Val cac gt c His Val 190 agc gct Ser Al a 205 gat ctg Asp Leu cac cgt His Ar g Thr act Thr aga Ar g acc Thr gtt Val ctt Leu gaa G u gt a Val 175 aat Asn at t l Ie aag Lys ttc Phe ctg Leu aaa Lys ggg G y cgt Ar g ggc G y t cc Ser t cc Ser 160 att SIe ggc G y gtc Val gta Val gac Asp 240 <210> 206 <211> 246 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 206 Leu lle Arg Asn Ala Cys Val Asn Asp Leu Thr Pro Asp Ser Oy n 1 5 10 G u lie Arg Asn Ala u Ser Leu G u Arg Phe Phe u Oy Thr Pro 25 Asn Val Lys IIe Thr Lys Leu G u Pro His Pro G y Arg Thr Ser IIe 40 Page 256 seql i st cor r ect ed. t xt lie Val Thr Val Pro G y Ser Asp Pro Asp Ala G u Pro Leu Thr Leu 55 Leu Asp Ser G n Val G u 145 Gy Asn Asp Lys Al a 225 Pr o Hi s Phe Asp Al a 115 Asp Hi s Ser Gy Gy 195 Gy Asp Thr Asp Al a Leu G u G u Asn Leu 165 Lys Sy Val SIle G n 245 Val 70 G u Phe Sy Al a Leu 150 Pr o Gy Ser Al a Tr p 230 Al a Val SIle SIle Sy Ar g 135 Phe Val Al a SIle Ar g 215 G n Pr o Ser Thr Leu 120 Gy Ser Hi s Al a Pr o 200 Ar g Gy Val Asp Al a 105 Ar g Gy Tr p Asp G n 185 Phe SIle Phe Asp Gy 90 Thr Gy Leu Lys Gy 170 Ar g Asp Al a Val Leu G n G n Thr Sy Asn 155 Ser Ar g Ar g Al a G n 235 Pr o SIle Al a Leu Al a 140 Cys Asp SIle Asp Al a 220 Al a Lys Tr p Al a Thr 125 Lys Leu Al a Hi s Ser 205 Asp Hi s Tr p Sy Val 110 Phe Tr p Ser Val Val 190 Al a Leu Ar g Thr Ar g Thr Val Leu G u Val 175 Asn SIe Lys Phe <210> 207 <211> 1308 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1285) <223> RXN03077 <400> 207 accccgatcc tttgttttcg tgggatcact attagactcg actctaccgc gctgcaggtt ttcctgatac gcctgcggac aaaacagaaa ggtatttcac gtg atg gaa att ggt Val Met Gu Ile y 1 gtg cag gtt gcc tca tgg atg gac cgc cac cat gac gag gtc ata aag Val G n Val Ala Ser Trp Met Asp Arg His His Asp G u Val Ile Lys 15 tgg cgc agg cat ttg cac agc cat cct gag ctc tcc cac atg gaa tac Trp Arg Arg His Leu His Ser His Pro G u Leu Ser His Iet G u Tyr 30 cgc acg act gag tat ttg gcc tcg gtt ctg aaa gat cac ggc atg gaa Arg Thr Thr G u Tyr Leu Ala Ser Val Leu Lys Asp His G y Iet G u Page 257 NO seql i st cor r ect ed. t xt 40 45 Scca cac ctg ttc cca gga acc ggt ttg atg gtg gat atc gga cca gaa 307 Pro His Leu Phe Pro G y Thr G y Leu 'b t Val Asp I I e G y Pro G u ,C 55 60 4 ggg gac tcc cgc ctg gcg ttt cgc gct gat atc gat gcc ctt ccg ctg 355 G3 y Asp Ser Arg Leu Ala Phe Arg Ala Asp Ile Asp Ala Leu Pro Leu 75 80 ctt gaa tca acc ggc tta gag ttc tct tcc aca gcc act ggc gtt gcg 403 Leu Gu Ser Thr G y Leu G u Phe Ser Ser Thr Ala Thr G y Val Ala C1 90 95 100 00 cat gcc tgc gga cat gac gtg cac acg gtg atc gct ttg gca ctt gcc 451 SHis Ala Cys G y His Asp Val His Thr Val Ile Ala Leu Ala Leu Ala 105 110 115 .C tgt gca ctg aac acc atc gaa ctg ccc atc ggc att cgg gtg att ttc 499 SCys Ala Leu Asn Thr Ile G u Leu Pro Ile G y Ile Arg Val Ile Phe 120 125 130 cag ccg gca gaa gaa gtc atg act ggt ggc gca acg gac gtc att gcc 547 Sn Pro Ala G u G u Val Ib t Thr G y G y Ala Thr Asp Val Ile Ala 135 140 145 cac ggt ggc ctt gat ggt gtg gat gcg att tac gcc atc cac gtt gaa 595 His G y G y Leu Asp G y Val Asp Ala Ile Tyr Ala lie His Val G u 150 155 160 165 ccc aaa ttg aag gtc ggt cgc gtc ggt gta cgc gct ggc gcg att act 643 Pro Lys Leu Lys Val G y Arg Val G y Val Arg Ala G y Ala le Thr 170 175 180 tct gcc tca gat gtg at c gaa atc aga gtc aag ggt gaa gga gga cat 691 Ser Ala Ser Asp Val Ile G u Ile Arg Val Lys G y G u G y G y His 185 190 195 agc gca cgt cca cac ctc tcc gct gat gtt gtt tac gcc ttg agc aaa 739 Ser Ala Arg Pro His Leu Ser Ala Asp Val Val Tyr Ala Leu Ser Lys 200 205 210 ttg gtc gtt gat ctt ccc ggt ttg ctg tcc agg cgc gtc gat cca cgc 787 Leu Val Val Asp Leu Pro G y Leu Leu Ser Arg Arg Val Asp Pro Arg 215 220 225 acc ggc acc gtg ctt gtt ttc ggc acc atc aac gcc ggc tat gcg ccc 835 Thr G y Thr Val Leu Val Phe G y Thr Ile Asn Ala G y Tyr Ala Pro 230 235 240 245 aac gcg atc cca gat tcc ggc atc gtg tca ggc acc ttg cgt aca gcc 883 Asn Ala ile Pro Asp Ser G y Ile Val Ser G y Thr Leu Arg Thr Ala 250 255 260 gac atc tct acc tgg cgt gac atg cgt ccg ctt atc tct gag ctg gtg 931 Asp Ile Ser Thr Trp Arg Asp 'b t Arg Pro Leu Ile Ser G u Leu Val 265 270 275 gaa cag gtg ctc gca ccc acc gga gtc acc cat gaa ctg atc tac aat 979 G u O n Val Leu Ala Pro Thr G y Val Thr His G u Leu Ile Tyr Asn 280 285 290 ccg ggt gtt cca cca gtg ctt aac gac gat gtc gcc acc gct ttg ttg 1027 Pro G y Val Pro Pro Val Leu Asn Asp Asp Val Ala Thr Ala Leu Leu 295 300 305 gca agc gca gca cgc gac atg gac aca caa tct gtt gtc caa gcg ccg 1075 Ala Ser Ala Ala Arg Asp 'b t Asp Thr n Ser Val Val n Ala Pro Page 258 seqI i st car r ect ed. t xt 310 315 320 cag tca tcc ggt gga gaa gac tt c tcg tgg tac ctt gaa cac gt c 0Gn Ser Ser 0Gy 0Gy 0u Asp Phe Ser Tr p Ty r Leu 0Gu Hi s Val 330 335 340 gga tca atg gcc cgg ttg ggt tgc tgg ccg ggg cac gga ccc aag 0 y Ser I'b t Al a Ar g Leu 0 y Cys Tr p Pr o 0 y Hi s a y Pr o Lys 345 350 355 gac ct c cat caa agt gac ct g gt t gt g gat gag cga gcc at c gga Asp Leu Hi s an ser Asp Leu Val Val Aspa0u Ar gAla Ilea0y 360 365 370 ggc gt c agg ct c t tt ggc t cc ct t gt g cag cag t ac agt agc cga 0ay Val Ar g Leu Phe 0ay Ser Leu Val a n a n Tyr Ser Ser Ar g 375 380 385 gaa gct t tc t ta aat t cc t aat gggggt agt gt gtagg gct a u Al a Phe Leu Asn Ser 390 395 <210> 208 <211> 395 <21 2> PRTF <21 3> Cor ynebact er i urn gi ut ani cumn 325 cca Pr o c aa a n gt t ValI t ct Ser 1123 1171 1219 1267 1308 <400> 208 Val It aullie As p Ser As p As p As p Al a Al a I Ie Thr 145 Al a Al a 0ay ValI vb t a y a y Le u a y Al a 115 ValI ValI Hi s Al a a y 1 95 a y Lys5 Tyr a u a u Le u Al a Al a Ph e Al a a u 1 65 Thr Hi s Val an Val Ar g Thr Hi s As p a u Al a Al a Pr o 135 a y Lys5 Al a Al a Al a Se r 10 Hi s Leu a u Ty r Phe Pr o Arg Leu Thr a y 90 a y His 1 05 Asn Thr a ua0u Leu Asp Lys Val 1 70 As p Val 1 85 Pr o Hi s Tr p Hi s Le u a y Al a Le u As p I Ie ValI a y 1 a y I Ie vb t Ser Al a Thr Ph e a u ValI a u vb t 140 ValI Ar g 0au As p Hi s Ser a y Ar g Ph e Hi s Le u 125 Thr As p ValI I Ie Ar g Pr o ValI Le u Al a Ser Thr 110 Pr a a y Al a a y Ar g 1 Hi s a u Le u vb t As p Ser ValI I Ie a y I Ie ValI 1 ValI Leu Ser Al a Asp Val Val 205 Page 259 Tyr Ala Leu Ser Lys Leu Val 210 215 seql i st cor r ect ed. t xt Val Asp Leu Pro Gy Leu Leu Ser Arg Ar g 225 Al a Thr SIle G u Al a 305 Val Leu Hi s Ar g Tyr 385 Asp Tyr Ar g G u 275 I le Al a G n Hi s Pr o 355 I le Ser Ar g Pr o 245 Al a Val Asn Leu Pr o 325 Pr o G n Val Ser Gy Al a l Ie G n Gy 295 Ser Ser Ser Leu Val 375 Al a Val Pr o Thr 265 Leu Pr o Al a Gy Al a 345 G n Leu Leu Val 235 Ser Ar g Pr o Val Asp 315 G u Leu Asp Gy Ser 395 Gy I e Met Gy 285 Asn Asp Phe Cys Val 365 Leu SIe Ser 255 Pr o Thr Asp G n Tr p 335 Pr o Asp G n <210> 209 <211> 1308 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(1285) <223> FRXA02855 <400> 209 accccgatcc tttgttttcg tgggatcact attagactcg ttcctgatac gcctgcggac aaaacagaaa ggtatttcac gt g Val tgg Tr p cgc Ar g cca Pr o gtt Val agg Ar g act Thr ct g Leu t ca Ser ttg Leu t at Tyr cca Pr o tgg Tr p cac Hi s tt g Leu gga G y at g Met agc Ser gcc Al a acc Thr cgc Ar g cct Pr o gtt Val ttg Leu cac cat Hi s Hi s 15 gag ctc G u Leu ctg aaa Leu Lys atg gt g Mvt Val Page 26C actctaccgc gctgcaggtt gtg atg gaa att ggt Val Met Gu Ile y 1 gac gag gtc ata aag Asp G u Val lie Lys t cc cac atg gaa tac Ser His Met G u Tyr gat cac ggc atg gaa Asp His G y Met G u gat atc gga cca gaa Asp lie y Pro u seql i st cor r ect ed. t xt ggg Gy ctt Leu cat Hi s tgt Cys cag G n cac Hi s 150 ccc Pr o tct Ser agc Ser ttg Leu acc Thr 230 aac Asn gac Asp gaa G u ccg Pr o gca Al a 310 t cc Ser t ca Ser tgc Cys ct g Leu 120 gca Al a ggc Gy ttg Leu t ca Ser cgt Ar g 200 gtt Val acc Thr at c SIle tct Ser gt g Val 280 gtt Val gca Al a ct g Leu ggc Gy cat Hi s acc Thr gaa G u gat Asp gt c Val 170 gt g Val cac Hi s ctt Leu ctt Leu gat Asp 250 tgg Tr p gca Al a cca Pr o cgc Ar g gcg Al a 75 tta Leu gac Asp at c SIle gt c Val ggt Gy 155 ggt Gy at c SIle ct c Leu ccc Pr o gtt Val 235 t cc Ser cgt Ar g ccc Pr o gt g Val gac Asp 315 ttt Phe gag G u gt g Val gaa G u at g let 140 gt g Val cgc Ar g gaa G u t cc Ser ggt Gy 220 ttc Phe ggc Gy gac Asp acc Thr ctt Leu 300 at g let cgc Ar g ttc Phe cac Hi s ct g Leu 125 act Thr gat Asp gt c Val at c SIle gct Al a 205 ttg Leu ggc Gy at c SIle at g Met gga Gy 285 aac Asn gac Asp gct Al a tct Ser acg Thr 110 ccc Pr o ggt Gy gcg Al a ggt Gy aga Ar g 190 gat Asp ct g Leu acc Thr gt g Val cgt Ar g 270 gt c Val gac Asp aca Thr gat Asp t cc Ser 95 gt g Val at c SIle ggc Gy at t l Ie gt a Val 175 gt c Val gtt Val t cc Ser at c SIle t ca Ser 255 ccg Pr o acc Thr gat Asp caa G n at c I e 80 aca Thr at c SIle ggc Gy gca Al a t ac Tyr 160 cgc Ar g aag Lys gtt Val agg Ar g aac Asn 240 ggc Gy ctt Leu cat Hi s gt c Val tct Ser 320 gat Asp gcc Al a gct Al a at t SIle acg Thr 145 gcc Al a gct Al a ggt Gy t ac Tyr cgc Ar g 225 gcc Al a acc Thr at c SIle gaa G u gcc Al a 305 gtt Val gcc Al a act Thr ttg Leu cgg Ar g 130 gac Asp at c l Ie ggc Gy gaa G u gcc Al a 210 gt c Val ggc Gy ttg Leu tct Ser ct g Leu 290 acc Thr gt c Val ctt Leu ggc Gy gca Al a 115 gt g Val gt c Val cac Hi s gcg Al a gga Gy 195 ttg Leu gat Asp t at Tyr cgt Ar g gag G u 275 at c SIle gct Al a caa G n ccg Pr o gtt Val 100 ctt Leu at t SIle at t SIle gtt Val at t l Ie 180 gga Gy agc Ser cca Pr o gcg Al a aca Thr 260 ct g Leu t ac Tyr ttg Leu gcg Al a ct g Leu gcg Al a gcc Al a ttc Phe gcc Al a gaa G u 165 act Thr cat Hi s aaa Lys cgc Ar g ccc Pr o 245 gcc Al a gt g Val aat Asn ttg Leu ccg Pr o 325 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 cag tca tcc ggt gga gaa gac ttc tcg tgg G n Ser Ser Gy Gy G u Asp Phe Ser Trp tac ctt gaa cac gtc cca Tyr Leu G u His Val Pro Page 261 seql i st cor r ect ed. t xt 330 335 340 gga tca atg gcc cgg ttg ggt tgc tgg ccg ggg cac gga ccc aag caa G y Ser IVet Al a Arg Leu G y Cys Trp Pro G y His G y Pro Lys G n 345 350 355 gac ctc cat caa agt gac ctg gtt gtg gat gag cga gcc atc gga gtt Asp Leu His G n Ser Asp Leu Val Val Asp G u Arg Ala lle G y Val 360 365 370 ggc gtc agg ctc ttt ggc tcc ctt gtg cag cag tac agt agc cga tct Sy Val Arg Leu Phe G y Ser Leu Val n G n Tyr Ser Ser Arg Ser 375 380 385 gaa gct ttc tta aat tcc taatgggggt agtgtgtagg gct G u Ala Phe Leu Asn Ser 390 395 <210> 210 <211> 395 <212> PRT <213> Corynebacter i um gl ut ai cum 1171 1219 1267 1308 <400> 210 Val Mvt G u 1 Asp G u Val Ser His IVet Asp Hi s G y Asp I e G y Asp Al a Leu Al a Thr G y Al a Leu Al a 115 lie Arg Val 130 Thr Asp Val 145 Ala lie Hi s Al a G y Al a Sy u y 195 Tyr Ala Leu 210 I e G y 5 I e Lys G u Tyr Met G u Pro G u Pro Leu Val Ala 100 Leu Al a I e Phe I e Al a Val G u 165 l e Thr 180 G y Hi s Ser Lys Val Gn Val Ala Ar g Thr Hi s 55 Asp G u Al a Al a Pr o 135 Sy Lys Al a Al a Val 215 Hi s 25 G u Phe Ar g Thr Sy 105 Asn G u Leu Lys Asp 185 Pr o Asp Tr p Hi s Leu Sy Al a Leu Asp SIle Val Sy 155 Gy I e Leu Pr o vet Ser Al a Thr Phe G u Val G u Met 140 Val Ar g G u Ser Sy 220 Asp Hi s Ser Sy Ar g Phe Hi s Leu 125 Thr Asp Val SIle Al a 205 Leu Ar g Pr o Val Leu Al a Ser Thr 110 Pr o Sy Al a Sy Ar g 190 Asp Leu Hi s G u Leu Met Asp Ser Val SIe Gy l e Val 175 Val Val Ser Arg Val Asp Pro Arg Thr y Thr Val Leu Val Phe y Thr Ile Asn Page 262 seql i st cor r ec 225 230 235 Ala y Tyr Ala Pro Asn Ala lie Pro Asp Ser 245 250 Thr Leu Arg Thr Ala Asp Ile Ser Thr Trp Arg 260 265 Ile Ser G u Leu Val G u G n Val Leu Ala Pro 275 280 G u Leu lie Tyr Asn Pro G y Val Pro Pro Val 290 295 Ala Thr Ala Leu Leu Ala Ser Ala Ala Arg Asp 305 310 315 Val Val n Ala Pro n Ser Ser y y u 325 330 Leu G u His Val Pro G y Ser bet Ala Arg Leu 340 345 His G y Pro Lys G n Asp Leu His G n Ser Asp 355 360 Arg Ala Ile y Val G y Val Arg Leu Phe y 370 375 Tyr Ser Ser Arg Ser G u Ala Phe Leu Asn Ser 385 390 395 <210> 211 <211> 1509 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(1486) <223> RXA00026 <400> 211 ccctttctgg ctagcctggg ctacattgtt ggcaatttgg tggacctggc tctgcgatct tcctcgaagg ataagttttc ted. txt G y lle Asp Ibet Thr G y 285 Leu Asn 300 IVet Asp Asp Phe Sy Cys Leu Val 365 Ser Leu 380 Ser 255 Pr o Thr Asp G n Tr p 335 Pr o Asp G n ttt Phe ggg Sy ggg Sy cag G n acg Thr cca Pr o gcc Al a agc Ser gca Al a cac Hi s gtt Val cgc Ar g gat Asp gag G u cat Hi s ccg Pr o aaa Lys t at Tyr tcg Ser ccg Pr o tcg Ser gcg Al a cca Pr o ttg Leu gaa G u act Thr gag G u ggc Sy 45 cgt Ar g gaa G u gt g Val gca Al a cag G n gat Asp gcg Al a tat ttg Tyr Leu 15 gaa t ct Su Ser cag gtt G n Val gaa ct g G u Leu ttc gag Phe G u Page 263 ttctacgccg atttgccgct atg agt act gac aat IVet Ser Thr Asp Asn 1 gat tac atg gag caa Asp Tyr Iet G u G n aac gcc agc acg aag Asn Ala Ser Thr Lys att tgg cgc ctg atc Ile Trp Arg Leu Ile cgc aca ctg gct ttc Arg Thr Leu Ala Phe gag gtg ttc gcc acc G u Val Phe Ala Thr NO seql i st cor r ect ed. t xt 75 80 gag gaa atc aca aaa ctt ctg caa aat cat ggt ttt gag gtt cag agt 403 O u u I I e Thr Lys Leu Leu 3 n Asn His G y Phe 3 u Val O n Ser 95 100 4 gga gtt tat ggt gtt aaa acc gct cta gaa act agt ttt gaa acc cct 451 G3 y Val Tyr y Val Lys Thr Ala Leu u Thr Ser Phe u Thr Pro 105 110 115 ggt tat gat cca gcg cag cac cca agc att gcg atc ttg gcg gaa tac 499 O y Tyr Asp Pro Ala O n His Pro Ser Ile Ala lie Leu Ala O u Tyr C- 120 125 130 00 gat gcc ctt cca gag atc ggc cat gca tgc ggg cac aat atc atc gca 547 SAsp Ala Leu Pro 3 u Ile G y His Ala Cys O y His Asn Ile Ile Ala 135 140 145 .C gca gct ggt gtt ggc gca ttt tta gct gtc acc aac atg atc aaa act 595 SAla Ala y Val y Ala Phe Leu Ala Val Thr Asn 'b t Ile Lys Thr S150 155 160 165 gcc gaa gtg aaa ggc gtg gat cac ctc gac ttt gaa ggc cgg atc gtg 643 Ala O u Val Lys O y Val Asp His Leu Asp Phe O u O y Arg Ile Val 170 175 180 ctg ttg gga aca cct gct gag gag ggg cat tcc ggc aag gaa tac atg 691 Leu Leu O y Thr Pro Ala O u O u O y His Ser O y Lys O u Tyr 'b t 185 190 195 atc cga aat ggc gca ttc gat ggc att gat gcg tcg att atg atg cac 739 Ile Arg Asn 3 y Ala Phe Asp 3 y Ile Asp Ala Ser Ile 'b t 'b t His 200 205 210 ccc ttt ggc ttc gat ctg gcg gag cat gtt tgg gtg ggc aga cgt acc 787 Pro Phe O y Phe Asp Leu Ala O u His Val Trp Val O y Arg Arg Thr 215 220 225 atg acg gcg acg ttc cac ggt gtc tct gca cac gcg tct tcg cag cct 835 'b t Thr Ala Thr Phe His G y Val Ser Ala His Ala Ser Ser O n Pro 230 235 240 245 ttc atg ggt aaa aat gcc ctc gac gct gca agt ttg gcg tac cag ggc 883 Phe 'b t y Lys Asn Ala Leu Asp Ala Ala Ser Leu Ala Tyr n y 250 255 260 ttc gga gtt ttg cgt cag caa atg cca ccg agc gac cgc ctt cac gcc 931 Phe G y Val Leu Arg n O n 'b t Pro Pro Ser Asp Arg Leu His Ala 265 270 275 att att acg gaa ggc gga aac cgg cca agc atc att cca gac act gca 979 Ile Ile Thr u y y Asn Arg Pro Ser Ile Ile Pro Asp Thr Ala 280 285 290 acg atg tcg ctg tac gtg cgt tct ttg ttg ccg gaa gca ctc aaa gac 1027 Thr 'b t Ser Leu Tyr Val Arg Ser Leu Leu Pro 0 u Ala Leu Lys Asp 295 300 305 ata tcg aaa cgc gtg gat gat gtg ctc gat ggg gcg gcc ttg atg gcg 1075 Ile Ser Lys Arg Val Asp Asp Val Leu Asp y Ala Ala Leu /b t Ala 310 315 320 325 ggg gtt ggc gtc gaa aag caa tgg gat gtg cac cca gct agc ttg ccc 1123 Sy Val y Val u Lys n Trp Asp Val His Pro Ala Ser Leu Pro 330 335 340 gtg cgc aac aat cat gtg ttg gcg cgg cgt tgg gca aaa acg cag aat 1171 Val Arg Asn Asn His Val Leu Ala Arg Arg Trp Ala Lys Thr n Asn Page 264 345 ctg cgt ggt cga acg gcg ctt Leu Arg y Arg Thr Al a Leu 360 gca gca tcg act gat ttt ggc Al a Al a Ser Thr Asp Phe G y 375 380 cat ccg atg gtg aaa att tct His Pro Met Val Lys Ile Ser 390 395 gaa ttc gcc gct tat gcg cgc G u Phe Ala Ala Tyr Al a Arg 410 gac gcc gca atc ggg ctg gcg Asp Al a Al a I e G y Leu Al a 425 ccg caa atg ctt atc gac gcg Pro n IVet Leu Ile Asp Ala 440 gta ctt aaa gta ggg gac tat Val Leu Lys Val G y Asp Tyr 455 460 ttc <210> 212 <211> 462 <212> PRT <213> Corynebacter i um gl ut ar seql i st cor r ect ed. t xt 350 355 tcg gag ggt att ttg ccc gac act Ser G u Gy lie Leu Pro Asp Thr 365 370 aat gtc tcg cac ctg gtt ccg ggc Asn Val Ser His Leu Val Pro G y 385 ccg gaa aac gtt gcg ctc cac acc Pro G u Asn Val Ala Leu His Thr 400 acg gaa gag gcc atc gac gca gcc Thr G u G u Ala Ile Asp Ala Ala 415 420 caa gtc gcc gtt gac gcg ctt gca G n Val Ala Val Asp Ala Leu Ala 430 435 acc ctc gag ttc acc aac tcc ggc Thr Leu G u Phe Thr Asn Ser y 445 450 ttg gct taggcaacga ctccgaaacc Leu Al a 1219 1267 1315 1363 1411 1459 1506 1509 <400> 212 IVet Ser Thr Asp Asn Phe Ser Pro n Val Pro Ser Thr Val 1 Asp Asn SIle Ar g G u Phe Ser SIle Hi s 145 Met Ser Ar g Leu Phe Val G u 115 Al a SIle 5 G n Lys SIle Phe Thr Ser Pr o Tyr Al a SIle G u G u Leu G u Val Tyr Al a 135 Al a Al a Ser 40 Al a Hi s SIle Tyr Asp 120 Leu Gy Al a Pr o Gy Asp Thr Gy 105 Pr o Pr o Val 10 Arg Lys Ala Asp Tyr Pro G u Ser Leu His Pro G u 75 Lys Leu Leu 90 Val Lys Thr Ala G n Hi s G u lie y 140 G y Al a Phe 155 Page 265 G u Gy Ar g G u G n Al a Pr o 125 Hi s Leu Al a G n Asp Al a Asn Leu 110 Ser Al a Al a Tyr Leu Su Ser G n Val G u Leu Phe G u Hi s G y G u Thr I e Al a Cys y Val Thr 160 seqi i st car r ect ed. t xt Asn l'bt I Ie Lys Thr Ala O31u Val 1 65 (31u G31y Ser ValI 225 A a Le u As p I Ie (31u 305 A a Pr o A a Le u Le u 385 A a I Ie As p Thr Ar g O31u 1 95 vb t Ar g Ser Tyr Le u 275 As p Le u Le u Ser Thr 355 As p Pr o Hi s A a Le u 435 Ser ValI vb t Hi s Thr Pr o 245 G31y A a A a As p A a 325 Pr o As n Le u I Ie Ly s 405 ValI As p As p Le u Ar g Ph e 215 Thr vb t Gy le b t 295 Ser ValI Ar g Ar g A a 375 Pr a Ph e A a O31n Le u 455 O31y As n 200 O31y A a O31y ValI Thr 280 Ser Ly s O31y As n O31y 360 Ser vb t A a A a vb t 440 Ly s Lys 03 y 1 70 Thr Pr o 1 03 y A a Phe As p Thr Phe Lys As n 250 Leu Arg 265 (31u (1y Leu Tyr Ar g Val Val O31u 330 As n Hi s 345 Ar g Thr Thr As p Val Lys A a Ty r 410 le G31y 425 Leu I I e Val O31v Val Asp Hi s Leu A a Ph e Le u Hi s 235 A a O31n G31y ValI As p 315 Ly s ValI A a Ph e I Ie 395 A a Le u As p As p O31u As p A a 220 G31y Le u O31n As n Ar g 300 As p O31n Le u Le u O31y 380 Ser Ar g A a A a Tyr 460 O31u O31y 205 O31u ValI As p vb t Ar g 285 Ser ValI Tr p A a Ser 365 As n Pr a Thr O31n Thr 445 Le u Asp Phe 1 Hi s Ser As p A a Val Tr p Al a Hi s 240 Al a Ser 255 Pr o Ser Ser I Ie Leu Pro As p (1y 320 Val Hi s 335 Ar g Tr p O31y I Ie Ser Hi s As n Val 400 O3 u A a 415 A a Val O3 u Phe <210O> 213 <21 1> 954 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (931) <223> RXA01971 Page 266 seql i st cor r ect ed. t xt <400> 213 aggtcttgtt tatttcggct actgattcag tagctgcgct ccgataggat tcttagtttt cagt t cagta t ctttgagcc acggctagaa tgtgaatcct atg tct aag aag aag Met Ser Lys Lys Lys cct Pr o gcg Al a gaa G u ggt Gy aat Asn gat Asp tgt Cys gat Asp tgg Tr p aat Asn 150 cct Pr o gcg Al a gt g Val ccg Pr o gag 230 ccc Pr o aca Thr gcc Al a gct Al a ttt Phe gct Al a gcc Al a gag G u 120 at t SIle gag G u aaa Lys gaa G u ttt Phe 200 t cc Ser ttt Phe ccg Pr o ttg Leu gag G u gcc Al a gcg Al a cgt Ar g ggt Gy acg Thr ttg Leu gat Asp acg Thr 170 ttg Leu cgt Ar g at t SIle ggg Gy gtt Val gca Al a gcg Al a gag G u tgt Cys 75 gcg Al a gag G u gcg Al a gca Al a gct Al a 155 at t SIle gat Asp aat Asn tt g Leu agt Ser 235 cct Pr o tcg Ser ggc Gy ctt Leu gcg Al a cgg Ar g act Thr gcg Al a at t l Ie 140 gat Asp ct g Leu cgt Ar g gag G u at t SIle 220 agg Ar g gcc Al a tgt Cys gt c Val 45 gcg Al a at t l Ie ct g Leu ggt Gy ttg Leu 125 agt Ser ttg Leu cat Hi s gga Gy gag G u 205 gaa G u aat Asn caa G n gga Gy gaa G u ct g Leu cat Hi s acg Thr ttg Leu 110 gat Asp gcg Al a at g Met tgt Cys t at Tyr 190 ttg Leu acc Thr gag G u ttt Phe 15 gga Gy aag Lys gag G u ccg Pr o cag G n 95 gat Asp gt g Val gat Asp cga Ar g ttt Phe 175 gt g Val cgg Ar g gat Asp ccg Pr o at c SIle gat Asp ctt Leu gcc Al a acg Thr 80 at g Met tcg Ser caa G n aag Lys gt g Val 160 tct Ser ttg Leu gag G u gcg Al a tcg Ser 240 1 cct Pr o ctt Leu tgt Cys gcg Al a aag Lys gcg Al a t at Tyr gag G u ccg Pr o 145 ttg Leu tcg Ser agt Ser gct Al a ccg Pr o 225 ttg Leu ggt Gy gca Al a acc Thr caa G n gct Al a gcg Al a tgg Tr p gag G u 130 ttg Leu gcg Al a ccg Pr o ttt Phe gct Al a 210 t at Tyr at t SIle at t I e ttg Leu ggt Gy ttt Phe cag G n ccg Pr o 100 aag Lys ct g Leu at t SIle gct Al a gac Asp 180 ggc Gy at t l Ie acg Thr cat Hi s gat Asp gtg Val gat Asp ggc Gy ttg Leu aat Asn cac Hi s cgc Ar g cac Hi s cca Pr o 165 gtg Val aat Asn gcg Al a ccg Pr o acg Thr 245 Page 267 gcg ct a t gc at t gcg gag gtt Al a Leu Cys Il e Al a 03 u Val1 250 gcg gct ttg aat gag aat ttt Al a Al a Leu Asn 03 u Asn Phe 265 t aacgt gagg t agct cacag t ca seql i st car r ect ed. t xt cgg ggg at g gct gt g gag gat gt t gcg Ar g 31y I'b t Al a Va 1 31u As p Val Al a 255 260 gat cgc gt t t at ggg gt c aca aat ct a As p Ar g Val Ty r G31y Val Thr As n Leu 270 275 <210O> 214 <211 <21 2> <21 3> 277 PmT Cor ynebact er i urn gi ut anri cumn <400> 214 I'bt Ser Lys Lys Lys Pr o Pr o Le u Cys Al a Ly s Al a Tyr O31u Pr o 145 Le u Ser Ser Al a Pr o 225 Le u Le u G31y ValI O31n As p As p I Ie 115 Al a vb t As p Le u Al a 1 95 Ar g vb t G31y 1 1 e Asp Leu Val 31y As p Phe 03 y O3 n Leu Pr o As n 1 00 Lys Hi s Leu Arg I e Hi s Al a Pr o 1 65 As p Val 1 80 31y As n I e Al a Thr Pr o Hi s Thr 245 Al a (31u G31y As n 70 As p Cy s As p Tr p As n 150 Pr a Al a ValI Pr a (31u 230 Al a Ar g Hi s Ar g Le u ValI G31y ValI Pr a Hi s 135 Ar g Pr a Ly s Thr I Ie 215 Pr a Le u Pr a Thr Al a 40 Al a Ph e Al a Al a (31u 120 li1e (31u Ly s (31u Ph e 200 Ser Ph e Cys I Ie Hi s 25 Ly s (31u Al a Al a I Ie 1 05 As p As p Al a As p Al a 1 85 Ly s O31n Ar g I Ie As n 265 Pr a 10 Le u O31u Al a Al a Ar g 90 G31y Thr Le u As p Thr 1 70 Le u Ar g I Ie G31y Al a 250 ValI Al a Al a O31u Cys Al a O31u Al a Al a Al a 1 li1e As p As n Le u Ser 235 O31u Al a Cys ValI Al a I Ie Le u G31y Le u 125 Ser Le u Hi s G31y O31u 205 O31u As n Ar g O31n G31y (31u Le u Hi s Thr Le u 110 As p Al a vb t Cys Tyr 1 Le u Thr (31u G31y Ph e G31y Ly s (31u Pr a O31n As p ValI As p Ar g Ph e 1 ValI Ar g As p Pr a vb t 255 Val 31u As p Al a Al a Al a Le u 03 u Asn Phe Asp Ar g Val Tyr 270 Page 268 seql i st car r ect ed. t xt 03 y Val Thr Asn Leu 275 <210O> 215 <21 1> 954 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (931) <223> RXA01802 <400> 215 ggaat t ct gg acaaaagt gt t cact acgt t agacat gaga gaaat ct tcg cgggt gt t ta gacaacccgg at gt gacaga accagt gt gg cacat cacag at g ggc gat [Vb t 31y As p at a I Ie g ac As p tt t Ph e cca Pr o gcg A a c ac Hi s c ac Hi s gt g ValI tt c Ph e g ac As p 1 50 ac a Thr t cc Ser gga G31y g ac As p g ac As p ag c Ser g aa O31u G31y at c I Ie t gc cys 120 gcc A a c ac Hi s c aa O31n aac As n g aa O31u ac a Thr ct g Le u cgt Ar g at t I Ie at t I Ie g at As p cca Pr o G31y gca A a gcc A a 1 70 aac As n t cc Ser ct t Le u gaa O31u caa O31n t ct Ser 75 at c I Ie gcc A a gaa O31u ct g Le u gt c ValI 155 gaa O31u gt t ValI aaa Ly s tt a Le u c aa O31n tt a Le u t ac Tyr t cc Ser gt g ValI cgc Ar g aaa Ly s 140 aac As n aag Ly s gt t ValI at g vb t ac c Thr cgt Ar g ac c Thr gca A a ac c Thr gca A a 110 t ac Tyr t ac Tyr g aa O31u t gt cys t cc Ser 1 90 g ac As p 15 c ac Hi s gcc A a tt g Le u g aa O31u ac c Thr 95 t cc Ser gt c ValI gt c ValI gt a ValI gcc A a 1 75 ggc (31y tt t Ph e c ac Hi s aaa Ly s c aa O31n aac As n 80 at c I Ie g ac As p g aa O31u t cc Ser at c I Ie 1 60 ccc Pr o C ag (31n 1 cgc Ar g g aa O31u gga O31y aaa Ly s ggc O31y g ac As p at c I Ie cgc Ar g at c I Ie 145 aag Ly s at c I Ie C ac Hi s ct c Le u gt c ValI at c I Ie C aa O31n ac c Thr t cc Ser g at As p aac As n 130 gag O31u gt a ValI at c I Ie t gg Tr p gt c ValI cca Pr o gcc A a tt c Ph e gt g ValI g ac As p at g vb t 115 g ac As p g aa O31u gcg A a cgc Ar g gt c ValI 1 caa gac O31n As p gcg aca A a Thr gag aaa O3 u Lys ttc gca Phe A a ggg cac O3 y Hi s gt a gt c Val Val acc gt a Thr Val 1 00 gga gt a O3 y Val gaa gct O3 u A a gt c caa Val O31n at c tt t I I e Phe 1 gca gcc A a A a 1 gat gt c As p Val Page 269 seqI i st car r ect ed. t xt at g gat cct t ca gcc aac aag ggc caa gct t t g gct gct I'b t Asp Pr o Ser Ala Asn Lys O31y O31n Al a Leu Al a Al a 200 205 210 gcc ct cggat t ggaa gaa tcc caa act ct cgtg t tt ggc Al a Leu G31y Leu O31u O31u Ser O31n Thr Leu Val Phe O31y 215 220 225 aac gac act gaa t tg at c aag gcc gcc ggc aag t ct t ac Asn Asp Thr 03 u Leu IlIe Lys Al a Al a 03 y Lys Ser Tyr 230 235 240 aat gcc cac ccg gac at t t tg gaa t tg gcc gac gaa at t Asn Al a Hi s Pr o Asp Ilie Leu 03 u Leu Al a Asp 03 u Ilie 250 255 aac at t gaa gag ggc gt t at t gt ggt gct ggag aag tt g Asn Ile (1u (1u 1y Val le Val Val Leu 1u Lys Leu 265 270 t aacgat tgc aggcagcagg tt c <210> 216 <211> 277 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn ct c Le u g ac As p gcc Al a gca Al a ct t Le u 275 cgc Ar g t ac Tyr at g vb t cca Pr a 260 aac As n <400> 216 [Vb t 31y As p (1n Ar g Leu Val Al a O31u ValI Pr o O31u O3 y I I e Al a Phe Lys 03 n Phe 03 y O3 y Thr Val Val As p Ser As p Thr 1 00 I Ie As p 'b t G31y 115 Ar g As n As p O31u 130 1I e 03 u 03 u Val 145 Lys Val Al a I Ie I Ie I Ie Ar g Al a 1 80 Hi s Tr p Val As p 1 95 Asp I I e Thr As p Lys Phe Al a Pr o Hi s Al a 70 Val Hi s Val Hi s Val Val Al a Phe O31n As p 150 Phe Thr 1 65 Al a Ser Val [Vb t I Ie vb t Tr p Al a G31y As p Ser ValI Ar g 135 Le u Ph e Pr a As p Lys5 G31y 25 li1e G31y Pr a O31u I Ie 1 05 Ar g O31u O31u As p Al a 1 85 Ser O31u 10 Thr Le u Ar g I Ie I Ie 90 As p Pr a G31y Al a Al a 1 70 As n Ser Le u O31u O31n Ser li1e Al a O31u Le u ValI 1 O31u ValI O31n As n vb t Al a I Ie Le u Ar g Al a 125 Tyr As n As p ValI (31y 205 vb t Thr Ar g Thr Al a Thr Al a 110 Tyr Tyr O31u cys Ser 1 As p Hi s Al a Le u O31u Thr Ser ValI ValI ValI Al a 1 O31y Al a Asn Lys 03 n Al a Leu Page 270 seqI i st car r ect ed. t xt Al a Al a Leu 210 Ar g Asp Al a Le u 215 G3 y Leu G3 u G3 u G n Thr Leu Val Phe a3 y Asp Tyr Leu As n 225 230 Asp Thr a3 u Leu I Ie 235 Lys Al a Al a a3 y Ser Tyr Al a [Vb t Ser 245 Asn Al a Hi s Pr o Il e Leu a u Leu Al a As p 255 a3 u IlIe Al a Ser Asn I I e a31ua(1u 265 a31y Val Ilie Val Val Leu a31u 270 Lys Leu Leu Asn a3 y 275 <210> <211> <212> <213> <220> <221> <222> <223> 217 1 066 DNA Cor ynebact er i urn CIDS (1066) RXN0 0866 gl ut ani cumn <400> 217 gcat caacgt aggagat cct cgact tccaa t tat ggct cc aaatgagcag gaacttgagg ct ct ccgaga agacat gaaa aaagct ggag t t ct at aaat at g b t 1 aat gat t cc Asn Asp Ser cga Ar g aat cgc ggc cgg Asn Arg ay Arg aag Lys5 gt t acc cgc aag Val Thr Arg Lys gcg Al a 15 ggc cca cca gaa a31y Pr o Pro a31u gct ggt Al a a3 y cag gaa aac a31na1u As n tct aac cag Ser Asn a3 n ct g gat acc cct Leu Asp Thr Pr o gt c ValI t tt cag gca cca Phe a31n Ala Pr o gat gct t cc Asp Al a Ser gac aat cgg Asp Asn Ar g agc gct gt a aaa Ser Al a Val Lys gct Al a gag acc gcc gga a3 u Thr Al a a3 y gat gct Asp Al a gcg caa ggt gct Al a a3 n a3 y Al a gga t cc caa gat a3 y Ser a3 n Asp cag ggt t cc cag a3 n a3 y Ser a3 aac Asn gct caa ggt t cc Al a a3 n a3 y Ser aac cgc gag t cc Asn Ar g a31u Ser gga a31y aac aac aac cgc Asn Asn Asn Ar g cgt t cc aac aac aac cgt cgc ggt ggt cgt Ar g Ser Asn Asn Asn Ar g Ar g a3 y a3 y Ar g 95 gga cgt cgt gga a31y Ar g Ar g a31y t cc gga Ser a3 y 1 00 aac gcc aat Asn Al a Asn ggc gcg aac aac a3 y Al a Asn Asn aac Asn 110 agc ggt aac cag Ser a3 y Asn a3 n aac cgt cag Asn Ar g a31n 115 ggc gga aac cgt ggc aac cgc ggt ggc gga cgc cga aac gtt gtt aag a31y a31y Asn Ar g a31y Asn Ar g a31y a31y a31y Ar g Ar g Asn Val Val Lys 120 125 130 t cg at g cag ggt gcg gat ct g acc cag Ser 'b t a31n a31y Al a Asp Leu Thr a31n cgc ct g cca gag cca cca aag Ar g Leu Pro a31u Pr o Pr o Lys Page 271 seql i st cor r ect ed. t xt 135 140 gca ccg gca aac ggt ctg cgt att tac Ala Pro Ala Asn y Leu Arg lie Tyr 150 155 atc ggt cgc aac atg acc gtg ttt gag lie y Arg Asn Met Thr Val Phe u 170 gtg gac tgt ggt gtg ctc ttc cca tct Val Asp Cys y Val Leu Phe Pro Ser 185 190 ctg att ctt cct gac ttc ggc cca att Leu lie Leu Pro Asp Phe y Pro lie 200 205 gat gca ttg gtg gtt act cac gga cac Asp Ala Leu Val Val Thr His G y Hi s 215 220 ccc tgg ctg ctg aag ctg cgc aac gat Pro Trp Leu Leu Lys Leu Arg Asn Asp 230 235 ttc acc ttg gct ctg att gca gct aag Phe Thr Leu Ala Leu Ile Ala Ala Lys 250 ccg aag ctg atc gag gtc aac gag cag Pro Lys Leu lie u Val Asn u n 265 270 ttc aac att cgc ttc tgg gct gtt aac Phe Asn lie Arg Phe Trp Ala Val Asn 280 285 ggt ctt gct atc aag act cct gct ggt Sy Leu Ala lie Lys Thr Pro Ala y 295 300 atc aag ctg gat cag act cct cct gat Ile Lys Leu Asp G n Thr Pro Pro Asp 310 315 <210> 218 <211> 322 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 218 IVet Asn Asp Ser Arg Asn Arg G y Arg 1 5 Pro Pro u Ala Gy Gn u Asn His 25 Ala Pro Asp Ala Ser Ser Asn G n Ser 40 G y Asn Asp Asn Arg Asp Al a Al a n 55 Ser n G y Ser G n Asn Al a n G y 70 gca Al a t ac Tyr 175 t ca Ser gag G u gaa G u at c SIle tgt Cys 255 t cc Ser cac Hi s ttg Leu ctt Leu 160 aac Asn ggt Sy gat Asp gac Asp cca Pr o 240 aag Lys aat Asn t cc Ser gt c Val ggc G y cgt Ar g cca Pr o ct g Leu 210 at t SIle ttg Leu cac Hi s gac Asp cca Pr o 290 cac Hi s t cc Ser ct c Leu 180 gtt Val cgc Ar g gct Al a t cc Ser cag G n 260 gga G y tgc Cys ggt G y gaa G u 165 at c SIe gac Asp gt c Val at t SIe cgt Ar g 245 cgt Ar g ccg Pr o ctt Leu gac Asp 595 643 691 739 787 835 883 931 979 1027 1066 gga cgc cca act Sy Arg Pro Thr 320 Lys Val Thr Arg Lys Ala y 10 Leu Asp Thr Pro Val Phe G n Ala Val Lys Ala G u Thr Ala G y Al a n G y Ser G n Asp Ser n Asn Arg u Ser y 75 Page 272 seql i st cor r ect ed. t xt Asn Asn Asn Arg Asn Arg Ser Asn Asn Asn Arg Arg y 90 G y Arg G y Ar g Asn Ar g Pr o 145 Gy Asn G u Hi s Hi s 225 I le G u Su I e I e 305 Pr o Gy Asn 115 Val Pr o SIle Leu Gy 195 Hi s Gy Al a Ar g Ar g 275 Asp Thr Gy G n Lys Lys G u 165 I le Asp Val SIle Ar g 245 Ar g Pr o Leu Asp Al a Gy Met 135 Pr o Gy Asp SIle Al a 215 Tr p Thr Lys Asn Leu 295 Lys Asn Asn 120 G n Al a Ar g Cys Leu 200 Leu Leu Leu Leu SIle 280 Al a Leu G u 105 Ar g Gy Asn Asn Gy 185 Pr o Val Leu Al a SIle 265 Ar g l Ie Asp Al a Asn Asp Leu 155 Thr Leu Phe Thr Leu 235 l le Val Tr p Thr Thr 315 Asn Ar g Leu 140 Ar g Val Phe Gy Hi s 220 Ar g Al a Asn Al a Pr o 300 Pr o Asn Gy 125 Thr SIle Phe Pr o Pr o 205 Gy Asn Al a G u Val 285 Al a Pr o Ser Gy Ar g Al a Tyr 175 Ser G u G u SIe Cys 255 Ser Hi s Leu Gy <210> 219 <211> 1045 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1045) <223> FRXA00866 <400> 219 gcatcaacgt aggagatcct cgacttccaa ttatggctcc aaatgagcag gaacttgagg ctctccgaga agacatgaaa aaagctggag ttctataaat atg aat gat tcc cga 115 IVet Asn Asp Ser Arg 1 aat cgc ggc cgg aag gtt acc cgc aag gcg ggc cca cca gaa gct ggt 163 Asn Arg Gy Arg Lys Val Thr Arg Lys Ala Gy Pro Pro G u Ala y Page 273 seql i st cor r ect ed. t xt ttt cag gca cca Phe n Ala Pro cag gaa aac n G u Asn t ct aac cag Ser Asn G n ctg gat acc cct Leu Asp Thr Pro gt c Val gat gct t cc Asp Al a Ser gac aat cgg Asp Asn Arg agc gct gta aaa Ser Al a Val Lys gct Al a gag acc gcc gga G u Thr Al a G y gat gct Asp Al a gcg caa ggt gct Al a n G y Al a gga tcc caa gat G y Ser G n Asp cag ggt tcc cag Sn G y Ser G n aac Asn gct caa ggt t cc Al a n G y Ser aac cgc gag tcc Asn Arg u Ser gga Gy aac aac aac cgc Asn Asn Asn Arg cgt tcc aac aac aac cgt cgc ggt ggt cgt Arg Ser Asn Asn Asn Arg Arg G y G y Arg 95 gga cgt cgt gga G y Arg Arg G y tcc gga Ser G y 100 aac gcc aat Asn Al a Asn ggc gcg aac aac G y Al a Asn Asn aac Asn 110 agc ggt aac cag Ser G y Asn G n aac cgt cag Asn Arg G n 115 ggc gga aac cgt ggc aac cgc ggt ggc gga cgc cga aac gtt gtt Gy Gy Asn Arg Gy Asn Arg Gy Gy Gy Arg Arg Asn Val Val 120 125 130 tcg atg Ser Vbet 135 cag ggt gcg gat G n y Ala Asp ctg Leu 140 acc cag cgc ctg Thr G n Arg Leu cca Pr o 145 gag cca cca aag G u Pro Pro Lys gca Al a 150 ccg gca aac ggt Pro Ala Asn G y cgt att tac gca Arg Ile Tyr Ala ggt ggc att tcc G y G y Ile Ser atc ggt cgc aac lie y Arg Asn at g Met 170 acc gtg ttt gag Thr Val Phe G u t ac Tyr 175 aac aac cgt ct g Asn Asn Arg Leu ct c at c Leu II e 180 gtg gac tgt Val Asp Cys ctg att ctt Leu IIe Leu 200 gtg ctc ttc cca Val Leu Phe Pro t ca ggt gag cca Ser Gy u Pro ggc gtt G y Val 195 cct gac ttc ggc Pro Asp Phe y cca Pr o 205 att gag gat cac Ile G u Asp Hi s ct g Leu 210 cac cgc gtc His Arg Val gat gca Asp Al a 215 ttg gtg gtt act Leu Val Val Thr cac Hi s 220 gga cac gaa gac G y Hi s G u Asp att ggt gct att I e G y Al a le ccc tgg ctg ctg aag ctg cgc aac gat atc cca Pro Trp Leu Leu Lys Leu Arg Asn Asp lie Pro 230 235 240 atc ttg gca tcc Ile Leu Ala Ser ttc acc ttg gct Phe Thr Leu Ala ct g Leu 250 att gca gct aag lie Ala Ala Lys aag gaa cac cgt Lys G u His Arg cag cgt G n Arg 260 ccg aag ctg Pro Lys Leu gag gtc aac gag G u Val Asn G u cag G n 270 tcc aat gag gac Ser Asn G u Asp cgc gga ccg Arg G y Pro 275 ttc aac att cgc ttc tgg gct gtt aac Phe Asn lie Arg Phe Trp Ala Val Asn cac tcc atc cca gac tgc ctt His Ser lie Pro Asp Cys Leu Page 274 seqI i st car r ect ed. t xt 280 285 290 ggt ct t gct atc aag act cct gct ggt t tg gt c at c cac acc ggt gac 31y Leu AlaIle Lys Thr Pr oAl aOyLeuVal Ile His Thr OyAsp 295 300 305 at c aag ct g gat cag act Il e Lys Leu Asp 03 n Thr 310 315 <210> 220 <211> 315 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 220 1 027 1 045 I'bt Asn Asp Ser Pr o Al a G31y Ser As n Ar g As n Ar g Pr o 145 G31y As n O31u Hi s Hi s 225 li1e (31u O31u As p As p G31y As n G31y As n 115 ValI Pr o I Ie Le u G31y 1 95 Hi s G31y Al a Ar g Ar g G31y Ser Ar g O31n As n G31y O31n Ly s Ly s O31u 1 65 li1e As p ValI I Ie Ar g 245 Ar g As n Ar g G31y O31n 1u As n Ser As n 03 n 40 As p Al a Al a As n Al a 03 n 70 Ar g Ser As n As n Al a As n O31y 1y As n 120 Ser I'b t 03 n 135 Al a Pr o Al a 150 li1e G31y Ar g Val Asp Cys Leu Il e Leu 200 Asp Al a Leu 215 Pr o Tr p Leu 230 Phe Thr Leu Pro Lys Leu Ar g Lys Val 10 Hi s Leu Asp Ser Al a Val O3 n 03 y Al a O3 y Ser 03 n Asn Asn Arg 90 O3 u 03 y Al a 1 Ar g G31y Asn O3 y Al a Asp Asn 03 y Leu 1 Asn I'b t Thr 1 70 G31y Val Leu 1 Pro Asp Phe Val Val Thr Leu Lys Leu 235 Al a Leu Il e 250 Ile 03 u Val 265 Thr Thr Ly s O31n As n Ar g As n Ar g Le u 140 Ar g ValI Ph e G31y Hi s 220 Ar g Al a Ar g Pr a Al a G31y Ar g G31y As n G31y 125 Thr I Ie Ph e Pr a Pr a 205 G31y As n Al a Al a Ph e Thr O31n Ser Ar g Ser O31y Ar g Al a Tyr 1 Ser O31u O31u I Ie Cys 255 As n 03 u 03 n Se r As n 270 Page 275 seqi i st car r ect ed. t xt Ou Asp Arg Gy Pro Phe Asn lie Arg Phe Trp Ala Val Asn His Ser 275 280 285 li1e Pro Asp Cys Leu G31y Leu Ala I Ie Lys Thr Pr o Ala G31y Leu Val 290 295 300 1 1 e Hi s Thr G3 y Asp I I e Lys Leu As p G3 n Th r 305 310 315 <210> 221 <211> 789 <212> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221> ODS <222> (766) <223> RXA02410 <400> 221 t at gagact g accat cct t g gaagct ct gg t agcgt gccc gct ccaggt a accccgcat c cggat at ctg t taact tct c cggacgcccc t gccgt gat t ggt G31y gt t ValI tt g Le u aat As n ag c Ser tt c Ph e gt c ValI gt a ValI gcg A a tt g Le u 1 50 ct t Le u tt c Ph e gt g ValI tt g Le u g at As p gcc A a ccg Pr o gag (31u 120 ac c Thr gag (31u gca A a cat Hi s cgc Ar g gga G31y cct Pr o g aa (31u cgc Ar g cgc Ar g gcg A a act Thr c aa O31n c ac Hi s tt c Ph e g aa G31u ggc G31y cgc Ar g gt g ValI ggc G31y at c I Ie 140 ggc G31y at t I Ie g at As p cca Pr o ac c Thr g ac As p cca Pr o ccc Pr a 110 t ca Ser gcc A a t ct Ser c aa O31n 15 c ac Hi s ac g Thr ccc Pr a g at As p gag O31u 95 at t I Ie at t I Ie gcc A a t gc Cys g at As p t gc Cys ct g Le u aac As n at g vb t 80 ct c Le u gag O31u ac g Thr cgc Ar g g at As p 1 60 at g vb t 1 cct Pr a gct A a gcc A a ag g Ar g t ca Ser at t I Ie ac c Thr t at Tyr aac As n 145 g ac As p gct A a g at As p gcc A a ct t Le u g at As p g at As p t ac Tyr t cc Ser 130 gt t ValI aaa Ly s gac at g ggc As p b t G31y g at As p tt t Ph e aag Ly s ggt G31y act Thr aat As n gcg A a 115 ggt G31y g ac As p gca A a gcg A a gcg A a ag c Ser cgt Ar g tt t Ph e tt c Ph e 1 00 ct t Le u g ac As p at t I Ie cca Pr a cca Pr a cat Hi s t cc Ser cgc Ar g tt g Le u gct A a ac g Thr cgc Ar g ag c Ser tt c Ph e gga G31y 1 at g cat at g t gt ggc caa gac gcc gga aga at t gcg gca gca gct ggc Page 276 Met His Met Cys G y G n Asp Ala C 170 gta aag aaa ctg att atc act cat g Val Lys Lys Leu Ile Ile Thr His 185 1 gcc aca gtg gca gca gct gcg gaa c Ala Thr Val Ala Ala Al a Ala G u H 200 205 gca cga tca gga atg gtt atc gag t Ala Arg Ser Gy Vet Val Ile u F 215 220 ggt <210> 222 <211> 222 <212> PRT <213> Cor ynebact eri um gl ut ai cum 1 i st cor r ect ed. t xt 3y Arg Ile Ala Ala Ala Ala y 175 180 itt cca cca tgg att gat gca gag (al Pro Pro Trp Ile Asp Ala G u 90 195 ac ttt gat ggt cct atc gaa ttg is Phe Asp Gy Pro lie u Leu 210 tt tagtccgttt gtactaataa 'he <400> 222 Mbt 1 Pr o Al a Al a Ar g Ser SIle Thr Tyr Asn 145 Asp Al a Tr p Gy Asp Iet y Al a Asp Al a Asp Phe Al a Ala Lys Ser Leu G y Ar g Asp Thr Phe Asp Asn Phe 100 Tyr Ala Leu 115 Ser G y Asp 130 Val Asp Ile Lys Ala Pro Al a Al a Al a 180 I e Asp Al a 195 Pro lie u Pro G y 5 His Val Ser Leu Arg Asn Leu Ser 70 Al a Phe Thr Val Arg Val Ser Al a Phe Leu 150 Sy bet 165 G y Val G u Al a Leu Al a Val SIle Met Leu 55 Ser Asp Thr G u Tyr 135 Cys Hi s Lys Thr Ar g 215 Al a Ser Tr p Phe G u Tr p Phe 105 Hi s G u Al a Cys Leu 185 Al a Gy Al a 10 Hi s Ar g Gy Pr o G u 90 Ar g Ar g Al a Thr Gy 170 I le Al a bet Val Leu Ar g Pr o Asp G u Val Thr Leu Tr p 155 G n SIle Al a Val G n Hi s Phe G u Gy Ar g Val Gy SIle 140 Gy Asp Thr Al a SIle 220 G u Thr Hi s Asp Val Lys Hi s Al a 125 Asp Thr Al a Hi s G u 205 G u G n Hi s Thr Pr o Asp G u I e I e Al a Cys Ar g 175 Pr o Phe <210> 223 Page 277 seql i st cor r ect ed. t xt <211> 455 <212> DNA <213> Corynebact eri um gl ut ai cum <220> <221> CDS <222> <223> RXA00961 <400> 223 cta gag aac Leu G u Asn 1 cac gca ccc His Ala Pr o gac gcc gag Asp Al a G u aac ct c gat Asn Leu Asp cca cgc tgc Pro Arg Cys tgg cat gtg Trp His Val caa gca gac G n Al a Asp t ac gat gca Tyr Asp Al a 115 atg cca caa lMt Pro G n 130 cgt Ar g 5 cag G n gt c Val gaa G u cgc Ar g ggc Gy aat Asn aag Lys ct g Leu at c SIle tac Tyr gcc Al a gag G u at c SIle 70 acc Thr ggc Gy cgt Ar g aag Lys ggc Gy ct c Leu ct c Leu gt a Val 55 caa G n gca Al a tgg Tr p gcc Al a gaa 35u 135 cgc Ar g gcc Al a ttc Phe 40 ct c Leu acc Thr cgt Ar g ttc Phe cac Hi s 120 tgg Tr p at g Met t ca Ser gct Al a gca Al a gt a Val ct c Leu at c SIle 105 at c SIle cgc Ar g ttg Leu 10 ggc Gy gcc Al a gag G u ggc Gy at c I e 90 t at Tyr gcc Al a t ac Tyr ct g Leu gcg Al a gac Asp gt g Val cgt Ar g 75 cgc Ar g gca Al a aac Asn gcc Al a ctt Leu gt c Val gct Al a gaa G u ttc Phe aac Asn gac Asp cct Pr o ct c Leu 140 ggc Gy at g Met gcg Al a gct Al a tgg Tr p gaa G u tgg Tr p gag G u 125 ct c Leu gac Asp gcc Al a cgt Ar g gaa G u gga Gy gtt Val ct g Leu 110 ct c Leu gaa G u gcc Al a at g Met gct Al a cgc Ar g gag G u ttc Phe tgg Tr p gga Gy cag G n gcc Al a gaa G u ggc Sy cga Ar g ct c Leu cgc Ar g ggt G y gaa G u aaa Lys tagcagcctc acctgttaag gga <210> 224 <211> 144 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 224 Leu G u Asn Trp Arg Ile G y Arg IVet 1 5 His Ala Pro Leu G n Tyr Leu Ala Ser 25 Asp Ala G u Ala Val Ala Leu Phe Ala 40 Asn Leu Asp Trp G u G u Val Leu Ala 55 Pro Arg Cys Ser Arg Ile G n Thr Val Leu Leu Leu G y Asp Al a Al a 10 Sy Ala Val let Al a lbt u Ala Asp Ala Ala Arg Ala y G u Val G u Ala G u Arg Arg Sy Arg Phe Trp y G u Leu Page 278 seqi i st car r ect ed. t xt 70 75 Tr p Hi s Val O31u G31y Th r Ala Arg Leu lieArg Asn Ou Val Phe Arg 90 (31n Ala As p Ar g As n G31y Tr p Phe I Ie Tyr Ala As p Tr p Leu Tr p (31y 100 105 110 Ty r As p Ala Ser Lys Ar g Ala Hi s I Ie Ala As n Pr o O31u Leu G31y O31u 115 120 125 l'b t Pr o O31n Ala Leu Lys G31u Tr p Ar g Ty r Ala Leu Leu G31u O31n Lys 130 135 140 <210O> 225 <21 1> 1116 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1093) <223> RXA00111 <400> 225 ccgagaagct ggagaaggcc aacaagcgt g gcctct acac ccggcgccat cactggcgac cactaaaaaa ggagacttcg ctccgcgtcc ttccacagcc l'bt A a Phe Phe Ser tt t Ph e gcc A a cgc Ar g gt c ValI ac c Thr g ac As p gcc A a ggc G31y ac g Thr ggc G31y ccc Pr o gt a ValI ccc Pr o cgc Ar g at c I Ie g ac As p 120 ccc Pr o aaa Lys5 at c gt t ValI gt c ValI ct a Le u cgc Ar g ac c Thr cgc Ar g gcc A a cgc Ar g ct g Le u ggc G31y ggc G31y gca A a cgc Ar g ct g Le u ct a Le u 45 ct c Le u at c I Ie cca Pr o t ac Tyr gcc A a 125 ct c Le u ag c Ser g ac As p cat Hi s c ac Hi s ct c Le u ggc G31y 110 gga G31y at c lIe 15 ag c Ser gga G31y tt a Le u ggg G31y gca A a 95 at g vb t g aa (31u ccc Pr a ac a Thr ccg Pr a gca A a gct A a 80 g at As p t cc Ser ct c Le u ag c Ser gcg A a ac c Thr 50 gca A a ggc G31y ggc G31y aaa Lys5 ag c Ser 130 t cc Ser 20 at t I Ie g at As p t cc Ser t gg Tr p c ac Hi s 1 00 cca Pr a at c I Ie aaa g aa O31u c ac Hi s ccc Pr a t ac Tyr gt c ValI ac a Th r gca A a gct ctc ggc cac gat gac gca ctt ctt gtc ggc tcc gac acc ggc gcc Page 279 seqi i st car r ect ed. t xt Ala Leu (31y Hi s Asp Asp Ala Leu Leu Val G31y Ser Asp Th r 03 y A a 135 ag c Ser 1 50 ct a Le u cga Ar g tt t Ph e ag c Ser cgc Ar g 230 t cg Ser gt t ValI ag a Ar g cga Ar g ct c Le u 310 t tc Ph e gcc A a t cc Ser aaa Lys5 t tg Le u 200 gct A a aac As n g ac As p t cg Ser aac As n 280 ac a Thr t ac Tyr cgc Ar g gct A a ggc G31y 1 70 c ac Hi s t ca Ser cgt Ar g t tc Ph e ac c Thr 250 ccc Pr o cga Ar g cca Pr o gag G31u ac g Thr 330 at c I Ie 155 gcg A a ct a Le u t t c Ph e gag G31u aca Thr 235 at c I Ie ag c Ser cgc Ar g t t c Ph e aac As n 315 tt t Ph e 140 gct A a at c I Ie c ac Hi s ct g Le u at c I Ie 220 gag G31u ac c Thr aaa Lys5 t gg Tr p ac c Thr 300 cct Pr o t cc Ser c ac Hi s gt c ValI cat Hi s 205 gt c ValI ac a Thr ccg Pr o ac a Thr g aa G31u 285 ac c Thr t cc Ser at g vb t ccc Pr o t ct Ser 1 90 aac As n aac As n gt g ValI at c I Ie gt c ValI 270 cat Hi s at c I Ie g aa G31u t ac Tyr ct t Le u 1 75 g ac As p ct c Le u aac As n ct c Le u at c I Ie 255 t cc Ser ct a Le u gcg A a tt t Ph e ccc Pr a 1 60 g ac As p ct c Le u tt c Ph e ac g Thr ct c Le u 240 cgc Ar g gca A a gcc A a at c I Ie gca A a 320 145 g aa G31u at g vb t ag c Ser c ac Hi s t cc Ser 225 cgc Ar g ac c Thr ccg Pr a aat As n ccc Pr a 305 gca A a cgg Ar g cga Ar g cga Ar g tt c Ph e 210 t cg Ser aaa Lys5 aac As n gt g ValI act Thr 290 ggc G31y ac c Thr gt c ValI cgc Ar g ct t Le u 1 gga G31y t cc Ser aaa Lys5 cgc Ar g t gg Tr p 275 gcg A a ggc G31y at c I Ie cgc Ar g gcc A a 1 gct A a at c I Ie t ac Tyr gca A a t ac Tyr 260 ct g Le u cgc Ar g t ac Tyr gca A a 595 643 691 739 787 835 883 931 979 1 027 1 075 1116 t aagcact gt ggct gaggcg ct g <210O> 226 <21 1> 331 <21 2> PRT <21 3> Cor ynebact <400> 226 l'b t A a Phe Phe G31y Se r Ar g Se r li1e Ala Se r I Ie Phe Thr Hi s Asp en urn gi Ser Phe Lys A a G31u Ar g Hi s Val ut anm cumn Ser Thr Ser Pro Leu Thr Ar g Leu I Ie Pro 10 Th r G31y Ala Lys Ar g Ar g Leu Ser Ser Thr 25 Se r Pro G31y I Ie IleAla Leu Asp G31y Pr o 40 Ser Val Ar g G31y lie Arg Leu His Leu Ala Page 280 seql i st cor r ect ed. t xt Ser Pro Thr Lys Pro Leu Val Leu li e Hi s G y G u Phe Al a Asp Ser Ser 145 G u et Ser Hi s Ser 225 Ar g Thr Pr o Asn Pr o 305 Al a Al a G y G y G y G y Phe Lys Pro 115 Ser Val 130 Asp Thr Arg Val Arg Arg Arg Leu 195 Phe G y 210 Ser Ser Lys Lys Asn Arg Val Trp 275 Thr Al a 290 GTy G y Thr li e Trp Tyr His Val 100 Pro Thr I e Al a G y Al a Arg G y 165 Ala I e 180 Ala Pro Ile Thr Tyr G n Al a Leu 245 Tyr Leu 260 Leu Leu Arg Thr Tyr G u Al a G u 325 70 Asp Al a Sy Al a Ser 150 Leu Ar g Phe Ser Ar g 230 Ser Val Ar g Ar g Leu 310 Phe Ty r Al a Tyr Leu 135 I e I e Ar g Ar g G u 215 Ser SIle Sy Thr Thr 295 Pr o Al a G u Asp 105 Leu Hi s Tr p Leu Pr o 185 Pr o Ar g Al a Hi s SIle 265 Thr Sy Leu Thr Leu 75 I e Ar g Hi s Asp SIle 155 Al a Leu Phe G u Thr 235 I le Ser Ar g Phe Asn 315 Phe Gy Sy Al a Al a 140 Al a l Ie Hi s Leu SIle 220 G u Thr Lys Tr p Thr 300 Pr o Pr o Tyr Al a 125 Leu Ser Hi s Val Hi s 205 Val Thr Pr o Thr G u 285 Thr Ser Al a Met G u Val Tyr Leu 175 Asp Leu Asn Leu SIle 255 Ser Leu Al a Phe Al a Asp Ser Leu Sy Pr o 160 Asp Leu Phe Thr Leu 240 Ar g Al a Al a SIe Al a 320 <210> 227 <211> 1020 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(997) <223> RXA01932 <400> 227 tttctaacct gcatccaagc ctaggtggaa ttgagatgac gcgtcgtaga gatcgaaaac tcaaccaaat ttcttgccta aggcttctag gattgtcgtt atg ctc ctt cac cca 115 Page 281 seql i st cor r ect ed. t xt IVet Leu Leu His Pro gat Asp cag G n cat Hi s acg Thr ggc Gy cac Hi s cga Ar g gca Al a act Thr gcg Al a 150 gt a Val aca Thr aag Lys cgc Ar g ct c Leu 230 gaa G u cag G n agt Ser gcg Al a tta Leu ggc G y ct a Leu gca Al a gt c Val 120 cga Ar g act Thr gct Al a acg Thr gcg Al a 200 gac Asp ccc Pr o cga Ar g t at Tyr ggt G y gat Asp ggg G y t cc Ser gtt Val gca Al a gcc Al a gca Al a caa G n cag G n 170 agc Ser gaa G u cga Ar g acc Thr t at Tyr 250 at c SIle aaa Lys caa G n gct Al a ggc G y 75 ggc G y cac Hi s gt a Val at t l Ie cag G n 155 gt g Val t at Tyr cgc Ar g ct c Leu at t SIle 235 gga G y gat Asp gac Asp gat Asp ggg G y ggc G y ct g Leu cca Pr o ttg Leu ggc G y 140 ct g Leu ctt Leu ct c Leu t ac Tyr t ca Ser 220 gt g Val caa G n acc Thr gct Al a at t SIle 45 gat Asp acc Thr ccg Pr o ttt Phe gat Asp 125 ggt G y cgt Ar g at t SIle aca Thr at c SIle 205 ccg Pr o t ac Tyr gct Al a ttg Leu cct Pr o gct Al a ttg Leu ct c Leu at c SIle cca Pr o 110 ggg G y gac Asp gaa G u ttt Phe ttt Phe 190 gaa G u ct a Leu ggc G y ct a Leu ccc Pr o 15 gtt Val ggg G y ccg Pr o aac Asn at c SIle 95 gcg Al a gt g Val agt Ser cgg Ar g ccg Pr o 175 ggc G y caa G n ct g Leu gaa G u ct a Leu 255 act Thr t ca Ser gt g Val tgt Cys at g Met 80 gcc Al a gct Al a tct Ser gcc Al a gct Al a 160 gt a Val aaa Lys t at Tyr gca Al a tgc Cys 240 gag G u ct c Leu gag G u ccg Pr o ttg Leu at c SIle at c l Ie at c l Ie ggg G y ggt G y 145 gt g Val act Thr gat Asp gcc Al a tct Ser 225 gac Asp gct Al a agc Ser gct Al a gt g Val gt g Val gat Asp agc Ser gac Asp ct g Leu 130 gga G y ggt G y gat Asp tgc Cys gat Asp 210 gat Asp gt g Val gga G y gcg Al a gat Asp agg Ar g t ac Tyr gcc Al a gt g Val gac Asp 115 agt Ser aat Asn tct Ser gtt Val t ac Tyr 195 ggg G y ttg Leu tta Leu aat Asn gag G u gca Al a gtt Val ttc Phe acg Thr gac Asp 100 gcg Al a at t SIle at t SIle act Thr t cc Ser 180 ct g Leu cac Hi s agc Ser gcc Al a t cc Ser 260 gag G u acc Thr t at Tyr cac Hi s gtt Val t ac Tyr ttt Phe gat Asp gcc Al a cct Pr o 165 act Thr aca Thr gac Asp gac Asp cat Hi s 245 gtg Val acg atg act gaa ttc aaa gga cag atc cac gcc ttt att aac cta ggg Page 282 seqI i st car r ect ed. t xt Thr I'bt Thr G u Phe Lys G y Gn Il e His Ala Phe HIe Asn Leu G y 265 270 275 gga at c agt t cc gat gcg cgg gct gct cga cga ct c at c cgc gcc gaa 979 a31y Ilie Ser Ser Asp Ala Arg Ala Al a Arg Arg Leu Ilie Ar g Al a a31u 280 285 290 t tg gaa gca gca ct t t gt t aaaggt tga gat t taacat t cg 1020 Leu a3 u Al a Al a Leu Cys 295 <210> 228 <211> 299 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn <400> 228 I'bt Leu Leu Hi s Pro Asp Le u a31u Pr o Le u li1e I Ie I Ie a31y a31y 145 ValI Thr As p Al a Ser 225 As p Al a Al a As p Ar g Tyr Al a ValI As p 115 Ser As n Ser ValI Tyr 1 95 a31y Le u Le u As n a31u Thr Tyr Hi s ValI Tyr Ph e As p Al a Pr o 1 65 Thr Thr As p As p Hi s 245 ValI a31n Hi s Thr a31y 70 Hi s Ar g Al a Thr Al a 150 ValI Thr Lys5 Ar g Le u 230 a31u Thr Al a ValI ValI Pr o a31y Ser Le u ValI Ser 135 ValI Le u Ser As p Thr 215 Pr o ValI a31n Ser Al a 40 Le u a31y Le u Al a ValI 120 Ar g Thr Al a Thr Al a 200 As p Pr a Ar g Thr Ph e Ph e Thr Ser Tr p ValI Pr a 1 05 Ser ValI Al a Hi s Pr a 1 85 vb t Pr a Thr Al a (31u 265 Tyr 10 a31y As p a31y Ser ValI 90 Al a Al a Al a a31n a31n 1 70 Ser a31u Ar g Thr Tyr 250 I Ie Lys5 a31n Al a a31y 75 a31y Hi s ValI I Ie a31n 1 55 ValI Tyr Ar g Le u I Ie 235 a31y Thr Al a I Ie As p Thr Pr a Ph e As p 125 a31y Ar g I Ie Thr I Ie 205 Pr a Tyr Al a Le u Pr a Al a Le u Le u I Ie Pr a 110 a31y As p a31u Ph e Ph e 1 a31u Le u a31y Le u Pr a ValI a31y Pr a As n I Ie Al a ValI Ser Ar g Pr a 1 a31y a31n Le u a31u Le u 255 Thr Ser ValI Cys vb t Al a Al a Ser Al a Al a 1 ValI Tyr Al a Cys 240 a31u Phe Lys 0 y 0n Ile Hi sAl a 270 Page 283 seqI i st car r ect ed. t xt Phe Ilie Asn Leu Gy y Ilie Ser Ser Asp Ala Arg Ala Ala Arg Arg 275 280 285 Leu I Ie Ar g Al a O31u L eu O31u Al a Ala Leu Cys 290 295 <210> 229 <211> 1131 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CIDS <222> (1108) <223> RXA02574 <400> 229 t gt gct cct t gcgggct gcg cagaagagcc ggaacagcaa aaagcaat aa gccgct t at c gacgt ccccc t ccacccct c ccgcaccgac cgcggaggat ttg gcg cgc gcg caa Leu Ala Arg Ala On at c I Ie gt t ValI G31y G31y gt c ValI ct t Le u g aa O31u cat Hi s ggt G31y gca Al a 1 50 at t I Ie g aa O31u aat As n tt t Ph e aat As n at c I Ie g at As p gt a ValI gt g ValI 120 ccc Pr o act Thr cca Pr o c aa O31n g at As p ggt O31y gag O31u tt c Ph e ccc Pr o g at As p gt t ValI gt t ValI gcc Al a tt c Ph e 1 70 cgc Ar g cag O31n t cc Ser gcg Al a gaa (31u 75 t ca Ser ct c Le u aac As n ggc G31y aca Thr 155 aaa Ly s g ac As p gca Al a t gg Tr p ct c Le u ggc G31y ccg Pr o gca Al a tt t Ph e g at As p 140 gct Al a cat Hi s c aa O31n tt g Le u ac a Thr 45 cgc Ar g ggc O31y cgc Ar g g aa O31u gca Al a 125 cgt Ar g tt t Ph e tt c Ph e gt c ValI g at As p g at As p g aa O31u cgc Ar g gt g ValI at c I Ie 110 cct Pr a t cc Ser gca Al a cca Pr a gcg Al a 15 gcg Al a g aa O31u gcc Al a gt c ValI at g vb t 95 ct a Le u gt t ValI tt t Ph e aag Ly s ggt (31y 1 75 t cg Ser ct c Le u aat As n gt c ValI c ag O31n 80 gcg Al a ggc O31y gt a ValI t cc Ser ggc O31y 1 60 c ac Hi s 1 ct g Le u aat As n ct g Le u ggc O31y 65 cgt Ar g c aa O31n act Thr g at As p aac As n 145 tt a Le u ggt (31y at g vb t c ag O31n ct c Le u 50 ag g Ar g gcc Al a ac c Thr ggt O31y gt a ValI 130 g ac As p ag c Ser cgt Ar g at g vb t ggg O31y 35 ac g Thr g at As p ac c Thr at g vb t tt a Le u 115 g at As p cca Pr a aaa Ly s gca Al a gt t ValI 20 gt g ValI g aa O31u tt c Ph e aat As n ac g Thr 1 00 gct Al a gct Al a gcc Al a gt a ValI agt Ser 1 gga O31y ggt O31y cct Pr a t cc Ser at t lIe ccg Pr a gca Al a t gg Tr p gt a ValI gga O31y 1 ggc (31y Page 284 seql i st cor r ect ed. t xt gat Asp act Thr gcc Al a gtt Val 230 gat Asp ct c Leu gt g Val t at Tyr agc Ser 310 cac Hi s gac Asp 200 at g Met t cc Ser cca Pr o gga G y gcc Al a 280 tcg Ser gaa G u caa gat gtg gtg acc ccc gca ctt G n Asp Val Val Thr Pro Ala Leu at c SIle ggc G y at c SIle ggc G y 250 agt Ser ct c Leu ggc Gy cct Pr o cct Pr o cac Hi s gac Asp 235 gt g Val gcc Al a aaa Lys t cc Ser caa G n 315 t at Tyr at g Met 220 ccc Pr o cct Pr o at t l Ie gca Al a gcg Al a 300 gaa G u ggt G y 205 at t SIle gcc Al a ttc Phe t cc Ser ggc Gy 285 at t l Ie caa G n 190 caa G n gtt Val acc Thr gat Asp gcc Al a 270 gca Al a gat Asp at g Met gca Al a cca Pr o t at Tyr ggc G y 255 acc Thr gac Asp cgc Ar g ct g Leu ctt Leu ggt G y caa G n 240 gt g Val cat Hi s caa G n gtt Val gca Al a 320 t ct Ser ctt Leu 225 ct g Leu at c SIle t ca Ser gca Al a gat Asp 305 tct Ser gat gag Asp u 195 gaa act G u Thr 210 ggc acc G y Thr ctc cgc Leu Arg tac acc Tyr Thr ccc gca Pro Al a 275 cta tgg Leu Trp 290 gct gcc Al a Al a gcg tta Al a Leu ctt Leu gac Asp gac Asp agt Ser gac Asp 260 gaa G u at c SIe gtt Val aga Ar g 691 739 787 835 883 931 979 1027 1075 caa ttg ctc tac atc aca cgt ctc gaa G n Leu Leu Tyr Ile Thr Arg Leu G u 330 ccc <210> 230 <211> 336 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 230 Leu Ala Arg Ala Gn Ile Pro Ou n 1 5 Leu IVet IVet Val y Val Ala Asn Tyr Asn n y Val Gy y Ile Phe Ile 40 Leu Leu Thr G u Pro G y Arg Asn Ile 55 Sy Arg Asp Phe Ser Val Ser Ile Asp 70 Arg Ala Thr Asn Ile Leu Oy Asp Phe caa aag G n Lys 335 tgaagttacc agtccgtaac 1128 1131 Sn Arg Asp 10 Asp G n Ala G y Ser Trp G u Al a Leu Phe G u G y Pro Ser Pro 90 Page 285 G n Leu Thr Ar g G y Ar g Al a Al a G u Al a Val Met O31n Th r I'b t Th r Pr o 31u O31n 1 00 seqI i st car r ect ed. t xt Val Ou Asp Leu Ala Ou I I e Leu (3 y 110 Thr As p As n 145 Le u G31y Le u Ser Le u 225 Le u I Ie Ser Al a As p 305 Ser Le u 115 As p Pr o Ly s Al a O31u 1 95 Thr Thr Ar g Thr Al a 275 Tr p Al a Le u Al a Tr p ValI G31y 1 65 G31y Ly s G31y G31y G31y 245 As p Al a As p Ser ValI 325 G31y Le u 135 Al a Thr Ser Tyr ValI 215 Pr o Tyr Ser Le u G31y 295 G31y Le u 1 05 Thr ValI Tyr ValI Thr 1 85 Le u ValI Ser G31y vb t 265 Ser Le u Tyr Tyr As n G31y Thr 1 Ly s As p Pr o Hi s As p 235 ValI Al a Ly s Ser O31n 315 Thr Ph e As p 140 Al a Hi s ValI Tyr vb t 220 Pr o Pr a I Ie Al a Al a 300 (31u Ar g Al a 125 Ar g Ph e Ph e ValI G31y 205 li1e Al a Ph e Ser G31y 285 li1e O31n Le u Pr a Ser Al a Pr a Thr 1 O31n ValI Thr As p Al a 270 Al a As p vb t O31u ValI Ph e Ly s O31y 1 Pr a Al a Pr a Tyr O31y 255 Thr As p Ar g Le u O31n 335 <210O> 231 <21 1> 1599 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (1576) <223> RXN00983 <400> 231 gt gagaaaac agt ggct caa at at cgacat ct t ct act ca cagt t caacc t gt cgt ggct ggaggccggc t gcat t ggt g t cgacgccga t gaaacgt cc gt g act gca ggt gaa 115 Val Th r Al a 31y O31u 1 acc acc act at g aat gt c acg t tg acc aat cct t tc gac aac gca att 163 Thr Thr Thr I'bt Asn Val Thr Leu Thr Asn Pro Phe Asp Asn Ala Ilie Page 286 I seql i st cor r ect ed. t xt 10 15 0 ttt gac cga gca gtc tcc ctt gaa cgt ccc gaa gga tgg caa gct gag 211 Phe Asp Arg Ala Val Ser Leu u Arg Pro u y Trp n Ala u 30 4 gat gtt cgt gtg tcg at c cca tct gga gaa tct gtc aca atc cca gtc 259 Asp Val Arg Val Ser Ile Pro Ser G y 3u Ser Val Thr Ile Pro Val 45 cag gtc aca gca ccg ctg gta gcc gac aac ggt gaa ctt cca gtg gag 307 Sn Val Thr Ala Pro Leu Val Ala Asp Asn G y u Leu Pro Val u C1 55 60 00 gtg tcc att ctt gat gga gca gac cgc tac acg ggt cgt ctc aat ctc 355 SVal Ser Ile Leu Asp y Ala Asp Arg Tyr Thr y Arg Leu Asn Leu S 70 75 80 .C act gtt cag ggt ggg caa gaa cct gca cca act t ca gtg aag gtg agc 403 SThr Val n G y G y n O u Pro Ala Pro Thr Ser Val Lys Val Ser 95 100 att cca aat ctc aag gac act tat gta gca ggg gag aag atc agc att 451 Ile Pro Asn Leu Lys Asp Thr Tyr Val Ala G y u Lys Ile Ser Ile 105 110 115 aac ttt gcg gtc aac aac ccg ttt gac gtt acg gtt aat tcg gtg cca 499 Asn Phe Ala Val Asn Asn Pro Phe Asp Val Thr Val Asn Ser Val Pro 120 125 130 agc ctg ggg gaa ggc gag aac tgg atg cct gca aac cta cgc gga ttt 547 Ser Leu y u y u Asn Trp /b t Pro Ala Asn Leu Arg y Phe 135 140 145 gat cca gag cag ggt act ccc aac tgt cgt tac aag aat t t a ggc gcg 595 Asp Pro u n G y Thr Pro Asn Cys Arg Tyr Lys Asn Leu G y Ala 150 155 160 165 aat aag agc tat gac tgc acc aca act acc tat gaa gtc agc gat ttg 643 Asn Lys Ser Tyr Asp Cys Thr Thr Thr Thr Tyr 0 u Val Ser Asp Leu 170 175 180 gat gta gaa cgc gga tac gtg gat att cca acg gta tgg acg ttt act 691 Asp Val u Arg G y Tyr Val Asp Ile Pro Thr Val Trp Thr Phe Thr 185 190 195 aac tcc gca ggc gaa acg gta tgg tcc aaa aac gtt gat gtg cct cga 739 Asn Ser Ala 3 y 3 u Thr Val Trp Ser Lys Asn Val Asp Val Pro Arg 200 205 210 gtt gaa ctc aat gga aca cag gat gct gtc act gat gca at c gta acg 787 Val u Leu Asn G y Thr n Asp Ala Val Thr Asp Ala le Val Thr 215 220 225 gtt gat ccc atc aac cca gtt cat tcc aac ggc cag agc caa act gtt 835 Val Asp Pro Ile Asn Pro Val His Ser Asn G y O n Ser O n Thr Val 230 235 240 245 gag gtc cag gct aat gtc acc t ca gag gga gat ctg cca gct gga tct 883 Su Val n Ala Asn Val Thr Ser u y Asp Leu Pro Ala y Ser 250 255 260 aag gtg gcc ttt tat cta gat tca tcg ccc att gat acc gca gct gtt 931 Lys Val Ala Phe Tyr Leu Asp Ser Ser Pro Ile Asp Thr Ala Ala Val 265 270 275 gat gcg gaa ggg cat gcc agc atc tcg att gat gtg gac aac atc gca 979 Asp Ala 3 u 3 y His Ala Ser Ile Ser Ile Asp Val Asp Asn Ile Ala Page 287 seql i st cor r ect ed. t xt 5 290 agc Ser cca Pr o 310 aca Thr cat Hi s gcg Al a at t SIle gcg Al a 390 gaa G u act Thr acc Thr gaa G u gt c Val 470 gt c Val 280 cag G n gat Asp ct g Leu gat Asp gcg Al a 360 ttt Phe gga Gy gag G u cag G n acc Thr 440 gaa G u ctt Leu gga Gy gaa cgc aca G u Ar g Thr 300 cca cga tca Pro Ar g Ser 315 gaa caa gtg G u G n Val 330 ttt tct gat Phe Ser Asp gca cac gat Al a Hi s Asp gtc aac ggt Val Asn y 380 gca aag ctt Ala Lys Leu 395 t at gaa gt a Tyr G u Val 410 acg ttc aaa Thr Phe Lys gaa caa ccg G u G n Pr o act ggt gtt Thr G y Val 460 gcg ct g ct g Ala Leu Leu 475 ctt aag ttg Leu Lys Leu 490 ttt Phe at c SIle cag G n gga Sy gga Gy 365 at t SIle cag G n aca Thr gt a Val t ca Ser 445 gct Al a gca Al a gag G u gcg Al a cag G n caa G n 350 tcg Ser gag G u ct a Leu gtt Val ctt Leu 430 gaa G u gga G y gcg Al a gtt cgc Val Arg cgt gat Arg Asp 320 aac t cc Asn Ser 335 aca aag Thr Lys ccg gtg Pro Val cgg gac Arg Asp gac ct c Asp Leu 400 gaa gcc G u Al a 415 gct ggt Al a G y act gag Thr G u agc tct Ser Ser ctt ggt Leu G y 480 gcc cga Al a Ar g 305 gcc ttg Al a Leu ttg gtg Leu Val act at t Thr Ile gct at c Ala I e 370 gtg gtt Val Val 385 aag cca Lys Pro gag ctg G u Leu gag gaa G u G u cag cct G n Pro 450 aac ggt Asn y 465 ggc atc G y lle ct c Leu gca Al a at c SIle gt c Val 355 ggt Sy cca Pr o gt a Val gat Asp gag G u 435 tct Ser ggc G y gt c Val gt c Val cgt Ar g at g Met 340 at c SIle act Thr act Thr aat Asn gaa G u 420 gaa G u gaa G u agt Ser ggt Sy gtt Val ttt Phe 325 aat Asn gca Al a ct c Leu aac Asn act Thr 405 ttg Leu ccc Pr o cct Pr o ttt Phe gca Al a 485 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1599 taggtggctg ggggcgtcga aaa <210> 232 <211> 492 <212> PRT <213> Corynebact <400> 232 Val Thr Ala y 1 Phe Asp Asn Al a Sy Trp n Al a er i um gl ut ari cum G u Thr Thr Thr Met Asn Val Thr Leu Thr Asn Pro 5 10 lie Phe Asp Arg Ala Val Ser Leu G u Arg Pro G u 25 G u Asp Val Arg Val Ser Ile Pro Ser Oy u Ser Page 288 seqi i st car r ect ed. t xt A a Asp As n 03 y ValI G31u O31y Ser O31u ValI As n 145 Ly s O31u ValI ValI As p 225 O31n Le u As p ValI A a 305 A a Le u Thr A a ValI 385 I Ie Pr o Le u Ly s I Ie 115 Ser Ar g Le u Ser Thr 1 95 ValI I Ie O31n A a A a 275 As n Le u A a I Ie ValI 355 O31y Pr o ValI (31u Le u Ser I Ie Pr o Ph e A a 1 65 Le u Thr Ar g Thr ValI 245 Ser ValI A a ValI Ph e 325 As n A a Le u As n O31n ValI 70 Thr I Ie As n Ser As p 150 As n As p As n ValI ValI 230 O31u Ly s As p Ser Pr o 310 Thr Hi s A a I Ie A a 390 Val Thr Ser I Ie Val O31n Pr o As n Phe A a 120 Leu 03 y 135 Pro O31u Lys Ser Val O31u Ser A a 200 O3 u Leu 215 As p Pr o Val O31n Val Ala A a 03 u 280 O31u (1n 295 O31uAs p Val Leu Pr o As p Lys A a 360 A a Phe 375 O31n (1y Ala Pro Leu Le u O31y Le u 1 05 ValI O31u O31n Tyr Ar g 1 85 O31y As n I Ie A a Ph e 265 O31y Pr a A a Ser ValI 345 Thr Ar g Thr As p O31y 90 Ly s As n O31y O31y As p 1 70 O31y O31u O31y As n As n 250 Tyr Hi s O31u Pr a O31u 330 Ph e A a ValI (31y O31n As p As n O31u Thr 1 cys Tyr Thr Thr Pr a 235 ValI Le u A a Ar g Ar g 315 O31n Ser Hi s As n ValI A a O31u Thr Pr a As n 140 Pr a Thr ValI ValI O31n 220 ValI Thr As p Ser Thr 300 Ser ValI As p As p O31y 380 As p Pr a Tyr Ph e 125 Tr p As n Thr As p Tr p 205 As p Hi s Ser Ser I Ie 285 Ph e I Ie O31n O31y O31y 365 li1e Ar g A a ValI 110 As p vb t cys Thr I Ie 1 Ser A a Ser O31u Ser 270 Ser O31u A a O31n O31n 350 Ser O31u Tyr Pr a A a ValI Pr a Ar g Thr 1 Pr a Ly s ValI As n O31y 255 Pr a I Ie ValI Ar g As n 335 Thr Pr a Ar g A a Lys 395 Leu 03 n Leu Asp Leu 400 Page 289 Ly s O31u O31u O31n As n 465 G31y ValI As p O31u 435 Ser G31y ValI Thr 405 Le u Pr o Pr o Ph e A a 485 As p A a Ser O31u 455 A a Le u seqi i st car r ect ed. t xt O31u O31u Ty r O31u ValI Th r Val O31u Ala 410 415 O31n Thr Thr Phe Lys Val Leu Ala (31y 425 430 Thr O31u O31u O31n Pr o Ser O31u Thr O31u 440 445 O31u Ser Thr G31y Val Ala G31y Ser Ser 460 Leu Leu A a Leu Leu A a A a Leu (3 y 475 480 O3 y Leu Leu Lys Leu 490 <210O> 233 <21 1> 1297 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 ODS <222> (1297) <223> FRXA00983 <400> 233 gt agaaaac agt ggct caa at at cgacat ct t ct act ca ggaggccggc t gcat tggt g t cgacgccga t gaaacgt cc cagt t caacc t gt cgt ggct gt g act gca ggt gaa 11! Val Thr Ala G1y Ou ac c Thr tt t Ph e g at As p c ag O31n gt g ValI act Thr at t I Ie aac As n act Thr cga Ar g cgt Ar g ac a Thr at t I Ie c ag O31n aat As n gcg A a 120 aat As n gt c ValI t cg Ser ccg Pr o g at As p ggg G31y aag Ly s aac As n gt c ValI t cc Ser at c SI e ct g Le u gga G31y 75 caa O31n gac As p aac As n ac g Thr ct t Le u cca Pr o gt a ValI gca A a g aa O31u act Thr ccg Pr o tt g Le u g aa O31u t ct Ser 45 gcc A a g ac As p cct Pr a t at Tyr tt t Ph e 125 ac c Thr cgt Ar g gga O31y g ac As p cgc Ar g gca A a gt a ValI 110 g ac As p aat As n 15 ccc Pr a g aa O31u aac As n t ac Tyr cca Pr a 95 gca A a gt t ValI cct Pr a g aa O31u t ct Ser ggt O31y ac g Thr act Thr ggg O31y ac g Thr 1 tt c Ph e gga O31y gt c ValI g aa O31u ggt O31y t ca Ser gag O31u gt t ValI g ac As p t gg Tr p ac a Thr ct t Le u cgt Ar g gt g ValI aag Ly s aat As n 130 aac As n c aa O31n at c I Ie cca Pr a ct c Le u aag Ly s at c I Ie 115 t cg Ser gca A a gct A a cca Pr a gt g ValI aat As n gt g ValI 1 00 ag c Se r gt g ValI Page 290 S agc ct g ggg gaa ggc gag aac t gg Se r L eu (31y (31u (31y 31u As n Tr p 135 140 eqi i st car r ect ed. t xt at g cct gca aac ct l'bt Pro Ala Asn Le a cgc u Ar g g at As p 1 50 aat As n g at As p aac As n gt t ValI gt t ValI 230 gag O31u aag Ly s g at As p ag c Ser cca Pr o 310 ac a Thr cat Hi s gcg A a at t I Ie gcg A a 390 gag G31u ag c Ser g aa O31u gca A a 200 ct c Le u ccc Pr o c ag O31n gcc A a g aa O31u 280 c ag O31n g at As p ct g Le u g at As p gcg A a 360 tt t Ph e gga O31y ggt (31y g ac As p 1 70 gga O31y g aa O31u gga O31y aac As n aat As n 250 t at Tyr cat Hi s g aa O31u cca Pr o g aa O31u 330 tt t Ph e gca A a gt c ValI gca A a act Thr 155 t gc CY S t ac Tyr ac g Thr aca Thr cca Pr a 235 gt c ValI ct a Le u gcc A a cgc Ar g cga Ar g 315 caa O31n t ct Ser cac Hi s aac As n aag Ly s 395 ccc Pr o ac c Thr gt g ValI gt a ValI c ag O31n 220 gt t ValI ac c Thr g at As p ag c Ser ac a Thr 300 t ca Ser gt g ValI g at As p g at As p ggt O31y 380 ct t Le u aac As n ac a Thr g at As p t gg Tr p 205 g at As p cat Hi s t ca Ser t ca Ser at c I Ie 285 tt t Ph e at c I Ie c ag O31n gga O31y gga O31y 365 at t I Ie c ag O31n t gt cys act Thr at t I Ie 1 90 t cc Ser gct A a t cc Ser gag O31u t cg Ser 270 t cg Ser gag O31u gcg A a c ag O31n c aa O31n 350 t cg Ser gag O31u ct a Le u cgt Ar g ac c Thr 1 75 cca Pr a aaa Ly s gt c ValI aac As n gga O31y 255 ccc Pr a at t I Ie gt t ValI cgt Ar g aac As n 335 ac a Thr ccg Pr a Ar g g ac As p t ac Tyr 1 60 t at Tyr ac g Thr aac As n act Thr ggc G y 240 g at As p at t I Ie g at As p cgc Ar g g at As p 320 t cc Ser aag Ly s gcg A a g ac As p aag Ly s g aa O31u gt a ValI gt t ValI g at As p 225 c ag (31n ct g Le u g at As p gt g ValI gcc A a 305 gcc A a tt g Le u act Thr gct A a gt g ValI 385 aat As n gt c ValI t gg Tr p g at As p 210 gca A a ag c Ser cca Pr a ac c Thr g ac As p 290 cga Ar g tt g Le u gt g ValI at t I Ie at c I Ie 370 gt t ValI tt a Le u ag c Ser ac g Thr 1 gt g ValI at c I Ie c aa G n gct A a gca A a 275 aac As n ct c Le u gca A a at c I Ie gt c ValI 355 ggt O3y cca Pr a gga ttt O3 y Phe ggc gcg (3 y A a 1 gat tt g Asp Leu 1 t tt act Phe Thr cct cga Pr o Ar g gt a acg Val Thr act gt t Thr Val 245 gga t ct 03 y Ser 260 gct gt t A a Val at c gca I e A a gt c gt t Val Val cgt ttt Ar g Phe 325 at g aat l'b t As n 340 at c gca I e A a act ct c Thr Leu act aac Thr As n 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 1297 Page 291 seqI i st car r ect ed. t xt <210O> 234 <21 1> 399 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 234 Val Thr Al a 31y O31u Thr Ph e G31y ValI O31u G31y Ser O31u ValI As n 145 Ly s O31u ValI ValI As p 225 O31n Le u As p ValI Al a 305 As n O31n li1e Pr o Le u Ly s I Ie 115 Ser Ar g Le u Ser Thr 1 95 ValI I Ie O31n Al a Al a 275 As n Le u le Ou ValI O31u Le u Ser I Ie Pr o Ph e Al a 1 65 Le u Thr Ar g Thr ValI 245 Ser ValI Al a ValI Thr Thr I'bt Asn 10 Asp Arg Ala Val Val Ar g Val Ser 40 Val Thr Al a Pr o Ser I Ie Leu As p Val O31n (1y (1y 90 Pro Asn Leu Lys 1 05 Phe Al a Val Asn 120 Leu 03 y 03 u 03 y 135 Pro O31u O31n G31y Lys Ser Tyr Asp 1 70 Val O31u Arg O31y 1 85 Ser Al a 03 y 03 u 200 O3 u Leu Asn 03 y 215 Asp Pr o Ilie Asn Val O31n Al a Asn 250 Val Al a Phe Tyr 265 Al a 03 u 03 y Hi s 280 O31u O31n Pr o O31u 295 O31u Asp Al a Pr o ValI Ser I Ie Le u O31y O31n As p As n O31u Thr 1 cys Tyr Thr Thr Pr o 235 ValI Le u Al a Ar g Ar g 315 Thr Le u Pr a ValI Al a O31u Thr Pr a As n 140 Pr a Thr ValI ValI O31n 220 ValI Thr As p Ser Thr 300 Ser Le u O31u Ser Al a As p Pr a Tyr Ph e 125 Tr p As n Thr As p Tr p 205 As p Hi s Ser Ser I Ie 285 Ph e I Ie Thr Ar g O31y As p Ar g Al a ValI 110 As p cys Thr I Ie 1 Ser Al a Ser O31u Ser 270 Ser O31u Al a As n Pr a O31u As n Tyr Pr a Al a ValI Pr a Ar g Thr 1 Pr a Ly s ValI As n O31y 255 Pr a I Ie ValI Ar g Ala Leu Ala Arg Phe Thr Val Leu Ser Ou On Val On On Asn Ser Page 292 seqI i st car r ect ed. t xt 325 330 335 Leu Val I Ie I'b t Asn Hi s Pr o Asp ValI Phe Ser Asp O31y O31n Thr Lys 340 345 350 Thr Ilie Val Ilie Al a Al a Lys Al a Thr Al a Hi s Asp G31y Ser Pro Al a 355 360 365 Al a Ile 1y Thr Leu Ile Al aPhe Ar gVal Asn (1y Ile (1u Ar gAsp 370 375 380 Val Val Pr o Thr Asn Al a 31n G31y Thr Al a Lys Leu O31n Leu Asp 385 390 395 <210> 235 <211> 440 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (417) <223> RXA00984 <400> 235 caa cgt ggt O31n Ar g G31y aaa at c gt g Lys IlIe Val t ca gga gcc Ser 03 y Al a gt c act t gg Val Thr Tr p aac aat gct Asn Asn Al a gcg cag gca Al a 03 n Al a cgc acc aat Ar g Thr Asn ccg gt g t ct Pr o Val Ser 115 gca acg ctt Al a Thr Leu 130 t at <210> 236 <211> 139 <21 2> PmT acc cca gt g Thr Pr o Val gaa ctc agc 03 u Leu Ser gat act t ac Asp Thr Tyr acc gag cca Thr O31u Pr o t cc ct g at t Ser Leu Ilie 70 aag gt t ct g Lys Val Leu ggc acc gt c O3 y Thr Val 1 00 aag gtg ttt Lys Val Phe ccc aac ggt Pr o Asn G31y ct c Le u g at As p cgc Ar g act Thr 55 cga Ar g ct g Le u cgc Ar g g aa O31u g ac As p 135 ct t Le u gga G31y aag Ly s 40 ct t Le u gt a ValI tt c Ph e at g vb t cca Pr o 120 ggg G31y ac c Thr gt g ValI g at As p tt c Ph e t cc Ser t cg Ser 1 gga G31y g aa O31u 10 ct g Le u t ct Ser ac c Thr ccg Pr o aac As n 90 t gt cys gca Al a act Thr at g vb t t at Tyr c ag O31n ac a Thr 75 act Thr g at As p at c I Ie cca Pr o aac As n t cc Ser ct g Le u gca Al a gcc Al a g at As p c aa O31n t gg Tr p aac As n ac c Thr ccg Pr a cct Pr a ac c Thr ggt G31y t at Tyr 125 aaa Ly s cgt Ar g ggc G31y cct Pr a gga G31y agt Ser ac c Thr t cg Ser ac a Thr t ca Ser ggc G31y cgc Ar g agt Ser ggc G31y t gg Tr p at g vb t at c ggc at g ct g I Ie G31y I'bt Leu tgagaaaaca gtggctcaaa 437 Page 293 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut ari cum <400> 236 G n Arg y Thr Pro Val Leu Leu Gy G u Thr Pro Tr 1 5 10 Lys Ile Val G u Leu Ser Asp y Thr Leu IVet Asn As Ser y Ala Asp Thr Tyr Arg Lys Val Ser Tyr Ser Th 40 4 Val Thr Trp Thr G u Pro Thr Leu Asp Thr G n Leu Pr 55 Asn Asn Ala Ser Leu Ile Arg Val Phe Pro Thr Ala Pr 70 Ala G n Ala Lys Val Leu Leu Phe Ser Asn Thr Ala Th 90 Arg Thr Asn G y Thr Val Arg Iet Ser Cys Asp Asp G 100 105 Pro Val Ser Lys Val Phe G u Pro Gy Ala lie n Ty 115 120 12 Ala Thr Leu Pro Asn G y Asp I e Gy Iet Leu 130 135 <210> 237 <211> 832 <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(832) <223> RXN02513 <400> 237 acagcaccgt tttgtaggat aagaaaatcc cgcacacaac ccgtcc ggggaggcat gtctatgccc ccaat t agac atctgacatc atg ct p n r o o r y r Lys Ar g Gy Pr o Gy Ser Thr Ser tggt gggtgaagtg t cca atc tgg 115 at g Met gt a Val acc Thr gca Al a gct Al a ctt Leu t ca Ser cca Pr o gca Al a ggc Gy ttc Phe ctt Leu gcc Al a at c l Ie act Thr aaa Lys gca Al a gct Al a gaa G u gga Gy gca Al a gcc Al a caa G n 45 gaa G u ttt Phe gct ttg Al a Leu 15 ct c at g Leu IVet ccc acg Pro Thr gag ttt G u Phe tgc tac Cys Tyr 80 Page 294 IVet Leu Pro lie Trp 1 tct cgg cgt aaa gca 163 Ser Arg Arg Lys Ala ggc gca gca cta ccc 211 Gy Ala Ala Leu Pro ggc tac gga ttc gat 259 G y Tyr G y Phe Asp tca aca caa caa ctc 307 Ser Thr G n G n Leu cgc atc cca tcg ctt 355 Arg lie Pro Ser Leu s ggc gtc gca ccc aac ggc aac gtc Oy Val Ala Pro Asn Oy Asn Val eql i st cor r ect ed. t xt ctc gca tcg tgg gat ggt Leu Al a Ser Trp Asp y aac aac tgt tca gat gct cca caa ccc aac tcc Asn Asn Cys Ser Asp Al a Pro n Pro Asn Ser 105 110 tcg acc gac aac gga gca acc tgg ggc gaa cag Ser Thr Asp Asn y Ala Thr Trp y u On I 120 125 ggt atc acc gcc gaa ccc aaa act ggc tat tcc Sy Ile Thr Ala u Pro Lys Thr y Tyr Ser 135 140 gtg gac tgg gag agg ggc gat gtc ttt aac ttc Val Asp Trp G u Arg G y Asp Val Phe Asn Phe I 150 155 160 gat gca gga tac ttc acc tcc caa cca ggc acg Asp Ala y Tyr Phe Thr Ser G n Pro y Thr 170 175 aac gtt gcc cat gtt gcc tac gcc aaa tca tca Asn Val Ala His Val Ala Tyr Ala Lys Ser Ser 185 190 tgg gtt gca gac acc gtc att act gat caa gtg Trp Val Ala Asp Thr Val lie Thr Asp G n Val 200 205 tgg gac agc cga ttt gcc aca tcc gga aac ggc Trp Asp Ser Arg Phe Al a Thr Ser y Asn G y 215 220 ggc gcg tac aag gga cga ttg gtc cag cca tcg Sy Ala Tyr Lys y Arg Leu Val G n Pro Ser 230 235 240 <210> 238 <211> 244 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 238 IVet Leu Pro Il e Trp Iet G y Leu Pro Phe Lys 1 5 10 Ser Arg Arg Lys Ala Val Phe Ser Ala Leu Gy 25 Sy Ala Ala Leu Pro Thr lie Pro Thr Ala G n 40 Sy Tyr y Phe Asp Al a Thr Ala Ser I e Ser 55 Ser Thr n G n Leu Al a Asp y y Thr Leu 70 Arg lie Pro Ser Leu y Val Ala Pro Asn y y 90 Trp Asp G y Arg Pro Asn Asn Cys Ser Asp Al a 100 105 Page 295 gt g Val gac Asp 130 ccc Pr o gt g Val ccg Pr o aac Asn gct Al a 210 caa G n cgc Ar g 100 aag Lys t cc Ser at c SIle t ca Ser gat Asp 180 t ca Ser gac Asp caa G n cca Pr o gta Val gca Al a gtt Val ttc Phe 165 cgc Ar g acc Thr acc Thr t ac Tyr gta act cgc atg Val Thr Arg IVet Lys Al a Al a G u Sy Asn Pr o Al a Al a G n G u Phe Val G n Sy Al a Thr Pr o Asp Leu Pr o 110 Al a Leu Pr o G u Cys Al a Asn SIle Hi s Asp 145 Hi s Asp Asp Val SIle 225 Val Gy 115 I le Ser Lys Asp Gy 195 Hi s Leu Ar g Val Al a Val Phe 165 Ar g Thr Thr Tyr Thr l Ie 135 Asp Al a Val Val Asp 215 Al a seql i st cor r ect ed. t xt Asp Asn y Ala Thr Trp 120 125 Thr Ala G u Pro Lys Thr 140 Trp G u Arg G y Asp Val 155 Sy Tyr Phe Thr Ser G n 170 Ala His Val Ala Tyr Ala 185 Ala Asp Thr Val lie Thr 200 205 Ser Arg Phe Ala Thr Ser 220 Tyr Lys y Arg Leu Val 235 G u Tyr Asn Gy 175 Ser G n Asn Pr o <210> 239 <211> 824 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (93)..(824) <223> FRXA02513 <400> 239 gtcatgtagg ataagaaaat cccgcacaca acccgtcctg gtggggtaag tgagggaggc atgtctatgc ccccaattag acatctgaca tc atg ctt cca atc tgg atg ggt IVet Leu Pro lle Trp IVet y 1 ctt cca ttc aag aaa gca ggg gct ttg tct cgg cgt aaa gca gta ttc Leu Pro Phe Lys Lys Ala Gy Ala Leu Ser Arg Arg Lys Ala Val Phe 15 tca gcg ctt ggt gca gac gca ctc atg ggc gca gca cta ccc acc atc Ser Ala Leu G y Ala Asp Ala Leu IVet Gy Ala Ala Leu Pro Thr lie 30 cca acg gcc caa gct caa aca ccc acg ggc tac gga ttc gat gca aca Pro Thr Ala G n Ala G n Thr Pro Thr Gy Tyr Gy Phe Asp Ala Thr 45 50 gca agc atc agc gaa gaa cca gag ttt tca aca caa caa ctc gct gac Ala Ser Ile Ser u u Pro u Phe Ser Thr n n Leu Ala Asp 65 ggc gga act ctc gga ttt gat tgc tac cgc atc cca tcg ctt ggc gt c G y y Thr Leu y Phe Asp Cys Tyr Arg lie Pro Ser Leu y Val 80 gca ccc aac ggc aac gtc ctc gca tcg tgg gat ggt cgc cca aac aac Al a Pro Asn G y Asn Val Leu Ala Ser Trp Asp G y Arg Pro Asn Asn 95 100 Page 296 113 161 209 257 305 353 401 seql i st cor r ect ed. t xt tgt Cys gac Asp 120 acc Thr tgg Tr p gga G y gcc Al a gca Al a 200 agc Ser t ac Tyr gat gct Asp Al a gga gca a y Al a gaa ccc G u Pro agg ggc Arg y 155 ttc acc Phe Thr 170 gtt gcc Val Ala acc gt c Thr Val ttt gcc Phe Al a gga cga G y Arg 235 cca caa ccc aac tcc atc gtg ggc Pro n Pro Asn Ser Ile Val y 110 115 acc tgg ggc gaa cag cac gac att Thr Trp G y u On His Asp Ile 125 130 aaa act ggc tat tcc gat ccc agc Lys Thr Gy Tyr Ser Asp Pro Ser 140 145 gat gtc ttt aac ttc cac gtg aag Asp Val Phe Asn Phe His Val Lys 160 tcc caa cca ggc acg gac ccg gat Ser G n Pro Gy Thr Asp Pro Asp 175 tac gcc aaa tca tca gat aac ggc Tyr Al a Lys Ser Ser Asp Asn G y 190 195 att act gat caa gtg gtt gct cat Ile Thr Asp G n Val Val Ala His 205 210 aca tcc gga aac ggc atc caa ctg Thr Ser G y Asn G y Ile G n Leu 220 225 aag gt a Lys Val t cc gca Ser Al a atc gtt Ile Val tca ttc Ser Phe 165 gat cgc Asp Arg 180 t ca acc Ser Thr gac acc Asp Thr caa t ac G n Tyr tcg Ser ggt G y gt g Val 150 gat Asp aac Asn tgg Tr p tgg Tr p ggc 30y 230 acc Thr at c SIe 135 gac Asp gca Al a gtt Val gtt Val gac Asp 215 gcg Al a ttg gtc cag cca tcg gta act cgc atg Leu Val G n Pro Ser Val Thr Arg IVet 240 <210> 240 <211> 244 <212> PRT <213> Corynebact <400> 240 IVet Leu Pro Il e 1 Ser Arg Arg Lys G y Ala Ala Leu G y Tyr G y Phe Ser Thr G n G n Arg Ile Pro Ser Trp Asp y Arg 100 I e Val y Lys 115 eri um gl ut arri cum Trp IVet y Leu Pro 5 Al a Val Phe Ser Al a 25 Pro Thr Ile Pro Thr 40 Asp Al a Thr Ala Ser 55 Leu Al a Asp G y G y 70 Leu G y Val Ala Pro Pro Asn Asn Cys Ser 105 Val Ser Thr Asp Asn 120 Phe Lys Lys 10 Leu G y Al a Ala G n Al a I e Ser G u Thr Leu G y Asn G y Asn 90 Asp Al a Pro G y Al a Thr Page 297 Al a Asp G n G u Phe Val G n Tr p 125 Sy Al a Thr Pr o Asp Leu Pr o 110 Sy Al a Leu Pr o G u Cys Al a Asn G u Hi s As p 145 Hi s As p As p ValI I Ie 225 ValI I Ie Ser Ly s As p O31y 1 95 Hi s Le u Ar g Al a ValI Ph e 1 65 Ar g Thr Thr Tyr I Ie 135 As p Al a ValI ValI As p 215 Al a seqi i st car r ect ed. t xt Thr Al a O31u Pro Lys Thr 140 Tr p G3 u Ar g G3 y Asp Val 1 G31y Ty r Phe Thr Ser O31n 1 70 Al a Hi s Val Al a Ty r Al a 1 Al a As p Thr Val I Ie Thr 200 205 Ser Ar g Phe Al a Thr Ser 220 Ty r Lys G31y Ar g Leu Val 235 Tyr As n G31y 1 Ser O31n As n Pr o <210O> 241 <21 1> 733 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 ODS <222> (733) <223> RXA00903 <400> 241 gcact cggaa t cgt cgt cct agaaaagaaa gacgcct aaa at gccct ct a aaat at cgcg gccct at tac caagt agat g t at tcagct c cgagccgtt c at g gga aac ccg ct t l'bt G31y As n Pro Leu gct Al a at c I Ie ac c Thr gag O31u ag g Ar g gcc Al a at c I Ie ag g Ar g g aa O31u ccc Pr o ct g Le u tt a Le u g at As p ac a Thr gct Al a gct Al a ggt G31y gct Al a gct Al a aac As n gac As p gga G31y gaa O31u 75 gt g ValI g at As p ct c Le u t ac Tyr c ac Hi s c ag O31n cgc Ar g g ac As p t ca Ser Cgg Ar g 45 cca Pr o gga G31y gct Al a tt a Le u g aa (31u gt a ValI ac a Thr ac c Thr at t I Ie gcc Al a ac a Thr at t I Ie gga G31y tt g Le u ggg G31y 1 g aa (31u Ph e tt c Ph e ac c Thr gt t ValI cca Pr o C aa O31n ct t Le u ac c Thr gcc Al a C ag O31n gca Al a at g vb t tt a Le u cca Pr a C ac Hi s g aa O31u agt Ser gcc Al a aag Ly s ac c Thr gt g ValI t gt cys gga O31y 1 00 gct ttt cag gct cca ccc aca ctc aaa gac ggg Ala Phe On Ala Pro Pro Thr Leu Lys Asp O1y cca ttg gat gct tcc Pr o Leu Asp Al a Ser Page 298 gac Asp cga Ar g ct a Leu 150 cat Hi s ccc Pr o cca Pr o gac Asp 120 cac Hi s agc Ser aca Thr gcc Al a aca Thr 200 gct Al a tgg Tr p caa G n aaa Lys 170 gaa G u agc Ser tgt Cys gt a Val cac Hi s 155 ct c Leu gt a Val ct c Leu gag G u gac Asp 140 gt a Val gga Gy cgg Ar g aat Asn seql i st cor r ect ed. t xt 110 gct tta gga atc agc ccc Ala Leu Gy Ile Ser Pro 125 130 aac ggc ccc ggc tgg gca Asn Gy Pro Gy Trp Ala 145 ttg gat ctg gaa ccc gat Leu Asp Leu G u Pro Asp 160 gtg att ggg gcc tat ccc Val Ile y Ala Tyr Pro 175 gca ttc gct caa gga atc Al a Phe Al a n G y Ile 190 gca ttc att gcg cag tgg Ala Phe lie Ala n Trp 205 210 115 gac Asp gt a Val ttc Phe gaa G u ggt Sy 195 ct a Leu ttc Phe gt g Val agt Ser ggg Sy 180 gaa G u att SIe gag G u gca Al a 165 gct Al a gac Asp <210> 242 <211> 211 <212> PRT <213> Corynebacter i um <400> 242 IVet y Asn Pro Leu Al 1 5 Su n Met Ala Arg II Phe Leu Leu Lys Pro TI Phe Thr Pro Thr Gy G Thr Ala His Val Phe Ar Val n G u Cys Val Al Pro Ala Ser y Leu Al 100 Pro Leu Asp Ala Ser As 115 Ser Pro Asp Phe Ile Ar 130 Trp Ala Val Val G u Le 145 15 Pro Asp Phe Ser Ala Hi 165 Tyr Pro G u G y Ala Pr 180 gl ut ai cum a e ir u g 70 a a iP g eu 50 S 0 Val Al a G n Leu G u Sy Phe Leu Al a 135 Pr o Pr o Hi s Al a Asp Al a 10 Trp Thr Asn G y Al a Asp Phe Al a G y Hi s G y G u 75 Val Al a Val 90 Ala Pro Pro 105 Al a Al a Cys Sn Trp Val Ala G n Hi s 155 Leu Lys Leu 170 Phe G u Val 185 Asp Ser Ar g Pr o Sy Al a Leu Al a 125 Asn Leu Val Ser Thr Ar g Leu G n Asp Asp Sy Pr o Leu Sy 175 Al a Thr SIe Sy Leu Sy Sy SIe Sy G u 160 Al a Arg Ala Phe Ala n 190 Page 299 seqi i st car r ect ed. t xt G31y I Ie 31y O31u Asp Pro Val Thr G31y Ser Leu Asn Ala Phe I Ie Ala 195 200 205 O31n Tr p Leu 210 <210O> 243 <21 1> 1146 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1123) <223> RXA01224 <400> 243 t t ggcggcgg gaagt t cagg ct t gggggca aacagt gct t ggat t t t aga caaaaaact c acggaagt ca t cct at ggca ggcgcgcct a ggat ggt gcc at g agc at c ct t l'bt Ser I Ie Leu ac g Thr act Thr tt g Le u gag O31u gcg A a gcg A a tt g Le u ccc Pr o gcc A a ccg Pr o 1 50 gt g ValI aaa Ly s gcg A a ggt O31y aaa Ly s aag Ly s cgg Ar g aat As n gt t ValI 120 at c I Ie g ac As p ggc (31y ccc Pr o gt c ValI gt c ValI gt c ValI t ca Ser tt t Ph e t gg Tr p t cc Ser gcc A a ct g Le u gag G31u 1 70 gt g ValI aat As n at g vb t tt t Ph e gac As p 75 ggc O31y gag O31u t gc Cy s acc Thr gct A a 155 gcg A a at t I Ie gca A a cct Pr o G31y at t I Ie ct c Le u gct A a ac c Thr gga G31y 140 gcg A a ggt (31y gt c ValI gca A a ct t Le u 45 gt c ValI g at As p g at As p aaa Ly s tt t Ph e 125 at t I Ie c ag O31n ggg (31y gcc A a gca A a gag O31u aac As n gag O31u gag O31u tt t Ph e 110 ggt G31y gag O31u aaa Ly s c ac Hi s ccg Pr a 15 gag O31u c ag O31n ct g Le u ct g Le u ccg Pr a 95 gag (31u at t I Ie gcg A a gct A a cgc Ar g 1 75 at g vb t gca A a ct g Le u tt t Ph e gcg A a ac g Thr gcc A a tt t Ph e t gg Tr p ggc G31y 1 t ct Ser 1 gct A a ggt G31y aaa Ly s cgc Ar g gga G31y gt g ValI gt t ValI ag c Ser gt g ValI 145 gcc A a ac c Thr ggc G31y t cc Ser c ag O31n ccg Pr a tt g Le u cct Pr a ct t Le u gct A a 130 ac g Thr aac As n t gg Tr p ggc O31y ct c Le u g aa O31u c ag O31n tt g Le u ac g Thr gcc A a 115 g aa O31u gt g ValI gcg A a t cc Ser ccg Pr a ggg O31y tt g Le u ac g Thr t cc Ser ccg Pr a 1 00 gct A a g aa O31u ac c Th r ct t Le u at t I Ie 1 Page 300 seqI i st car r ect ed. t xt gt g ValI c aa O31n t cc Ser t gt CYS 230 aac As n t ca Ser tt c Ph e cca Pr o g at As p 310 ggt (31y ccg Pr o G31y 200 g ac As p t cc Ser gag (31u cgc Ar g cgt Ar g 280 at c I Ie gcc Al a gca Al a gac gag cgc gac Asp O31u Ar g Asp 1 85 gt t t ac ct c ccg Val Tyr Leu Pr o gt g gca gca at t Val Al a Al a Ilie 220 gcc t tt t tg ct t Al a Phe Leu Leu 235 at c t tg gac gcc Il e Leu Asp Al a 250 gca t tt t cg ggc Al a Phe Ser 03 y 265 t cg aac gag ggg Ser Asn 03 u 03 y aca t ct t ta cgt Thr Ser Leu Ar g 300 t ac t gc ct g gt a Tyr Cys Leu Val 315 aag cag at a ct g Lys 03 n Il e Leu 330 ct g aaa acc ct c ct c gca gct Leu Lys Thr Leu Leu Al a Al a 190 195 ctc atc gca gcc ggc ggc ctt Leu Il e Al a Al a 03 y 03 y Leu 205 210 tta gaa gcc ggc gcc agc gct Leu 03 u Al a 03 y Al a Ser Al a 225 agc gac gaa gcc ggc acc agc Ser Asp 03 u Al a 03 y Thr Ser 240 gcc cca gca ct t ggt t tg gaa Al a Pr o Al a Leu G31y Leu 031u 255 cgt t at gcc agg gga gt g gaa Ar g Tyr Al a Ar g G31y Val 031u 270 275 t ta ccc ccg t tg t ac cca t ac Leu Pr o Pr o Leu Tyr Pr o Tyr 285 290 aag gt g gcg gga agt gca ggg Lys Val Al a G31y Ser Al a G31y 305 gga gt c ggc ct g gaa t cg at t G31y Val G31y Leu 031u Ser Ilie 320 gaa t ca t ta aca cct t cc gct 031u Ser Leu Thr Pr o Ser Al a 335 gtc ValI t ca Ser gcc Al a t ca Ser t cg Ser 260 c Thr ct c Le u aac As n gcg Al a tt g Le u 340 691 739 787 835 883 931 979 1 027 1 075 1123 1146 t aat gt tggg gggagt gct t t ca <210O> 244 <21 1> 341 <21 2> PRT <21 3> Cor ynebact er i urn gI ut anri cumn <400> 244 I'bt Ser I I e Leu Asp Thr Leu Lys Thr 1 5 Ala G1y Gy Pro Ser Thr Pro Al a Leu 25 G31y Se r Leu G31y Phe L eu Al a 31y G31y 40 Lys O31n 031u Leu Ser 031u ValI Lys G31y 55 Arg Pro O31n Thr As p Al a Pro Lys Pr o 70 G31y Leu Leu Ser Ser Al a Phe Ar g O31n Pro Val I Ie 10 Val As n Al a Val I'bt Pr o Val Phe G31y Ser As p I I e Phe 03 y Leu 90 Page 301 ValI Al a Le u ValI As p As p Al a Al a 031u As n (31u (31u Pr o 031u O31n Le u Le u Pr o seqIi Val Pro Thr Pro Asp Leu Ser As 1 00 st car r ect ed. t xt Trp (31u Al a Lys n ValI Ser ValI 145 Al a Thr Le u G31y Al a 225 G31y G31y G31y Tyr Ser 305 (31u Pr o Al a 115 (31u ValI Al a Ser Al a 1 95 Le u Al a Ser O31u O31u 275 Tyr Gy le Al a Lys5 Ph e As n ValI 1 65 O31u Lys5 Thr Ser Le u 245 ValI Ar g As n Tr p Lys5 325 G31y Pr o Al a Pr o 150 ValI ValI O31n Ser Cy s 230 As n Ser Ph e Pr o As p 310 G31y Al a Ar g 135 O31u O31n O31u Al a Al a 215 G31y Ar g Ser Thr vb t 295 Tyr Ser ValI 120 li1e As p G31y Pr o G31y 200 As p Ser O31u Ar g Ar g 280 li1e Al a Al a (31y 1 05 Ph e Lys5 Al a Pr a As p 1 ValI ValI Al a I Ie Al a 265 Ser Thr Tyr Lys5 Phe 03 u Al a 110 Ser Al a Le u O31u 1 70 O31u Tyr Al a Ph e Le u 250 Ph e As n Ser cys O31n 330 cys Thr Al a 1 55 Al a Ar g Le u Al a Le u 235 As p Ser (31u Le u Le u 315 li1e Ph e 125 li1e O31n O31y Le u Le u 205 Le u Ser Al a Ar g Le u 285 Lys5 O31y (31u I Ie Al a Al a Ar g 1 Thr Al a Al a O31u Al a 255 Al a Pr a Al a O31y Le u 335 Ph e Tr p O31y 1 Ser Le u Al a O31y Al a 240 Le u Ar g Le u O31y Le u 320 Th r <210O> 245 <21 1> 723 <212> DNA <213> Cor ynebact er i urn gi ut anri cumn <220> <221 CIDS <222> (700) <223> RXA01571 <400> 245 aaact acct g ct gagagct t t gt aat t t ac ggt gt ggt t g t ggaggggt g cgt cgagaag cgct cgt agg cgctt t tgat tt t tcggt ag gct aact ggg gt g agt at c t ca gt a 115 Val Ser I I e Ser Val 1 aaa gca cta caa aag tcc ggc cca gaa gca cct ttc gag gtc aag atc 163 Lys Ala Leu On Lys Ser Oy Pro Ou Ala Pro Phe Ou Val Lys Ilie Page 302 I seqI i st car r ect ed. t xt 15 at t gaa cgc cgt gac cca cgc gca gat gat gt g gt t at t gat at c aaa 211 I Ie G31u Ar g Ar g Asp Pr o Ar g Al a Asp Asp Val Val I Ie Asp I Ie Lys 30 Sgct gcg ggc atc tgc cac agc gat atc cac acc atc cgc aac gaa tgg 259 Ala Ala (1y Ile Cys Hi s Se r Asp Ilie Hi sThr Ile Ar gAsn (1u Tr p 45 ggc gag gcg cac ttc ccg ctc acc gtc ggc cac gaa atc gca ggc gtt 307 G31y 1u Al aHi s Phe Pr o Leu Thr Val G31y Hi s 31u Ile Al a 1y Val c-155 60 00 gt c t ct gcg gt t gga t cc gat gt a acc aaa t gg aaa gt c ggc gac cgc 355 SVal Ser Al a Val G31y Ser Asp Val Thr Lys Tr p Lys Val G31y Asp Ar g S 70 75 80 1.C gt g ggc gt c ggc t gc ct c gt t aac t cc t gc ggc gaa t gc gaa cag t gc 403 SVai31y Va31y Cys Leu VaiAsn Ser Cys (1y (1u Cys (1u (1n Cys 95 100 gt c gca gga t tt gaa aac aac t gc ct t cgc gga aac gt c gga acc t ac 451 Val Al a G31y Phe (31u Asn Asn Cys Leu Ar g G31y Asn Val G31y Thr Tyr 105 110 115 aac t ct aac gac gt C gac ggc acc at c acc caa ggc ggc t ac gct gaa 499 Asn Ser Asn Asp Val Asp G31y Thr Ilie Thr O31n 31y G31y Tyr Al a G31u 120 125 130 aag gt a gt g gt c aac gaa cgt t tc ct g t gc agc at c cca gag gaa ct t 547 Lys Val Val Val Asn G31u Ar g Phe Leu Cys Ser Ilie Pr o 31u G31u Leu 135 140 145 aac ttc gat gtc gca gca cca ctg ctg tgc gca ggc atc acc acc tac 595 Asn Phe Asp Val Al a Al a Pr o Leu Leu Cys Al a 31y Ilie Thr Thr Tyr 150 155 160 165 tcc cca atc gct cgc tgg aac gtt aaa gaa ggc gac aaa gta gca gtc 643 Ser Pr o Ilie Al a Ar g Tr p Asn Val Lys G31u G31y Asp Lys Val Al a Val 170 175 180 at g ggc ct c ggc ggg act cgg aca cat ggg t gt cca gat cgc t gc agc 691 [Vb t G31y Leu G31y G31y Thr Ar g Thr Hi s G31y Cys Pr o Asp Ar g Cys Ser 185 190 195 caa ggg t gc t gaggt tacc gt tct gtccc gt t 723 (31n 1y Cys 200 <210> 246 <211> 200 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 246 Val Ser IlieSer Val Lys Ala Leu On Lys Ser Gy Pro Ou Ala Pro 1 5 10 Phe (1u ValI Lys Ilie I Ie 1u Ar gAr gAs p Pro Arg AiaAsp Asp Val 25 Val Ile Asp Ilie Lys AiaAia Oy Ilie Cys His Ser Asp Ilie HisThr 40 Ile Ar gAs n 1u Tr p (1y 1u Ala Hi s Phe Pro Leu Thr Val (3y Hi s 55 Page 303 G u Lys G u Asn Gy SIle 145 Gy Asp Pr o Al a Gy G u Gy 115 Tyr G u Thr Val Ar g 195 Val Ar g Cys Tyr G u Leu Tyr 165 Val Ser Ser Gy Al a Ser Val 135 Phe Pr o Gy Gy seql i st cor r ect ed. t xt Ala Val Gy Ser Asp Val Val G y Cys Leu Val Asn 90 G y Phe G u Asn Asn Cys 105 Asn Asp Val Asp Gy Thr 120 125 Val Val Asn G u Arg Phe 140 Asp Val Ala Ala Pro Leu 155 lie Ala Arg Trp Asn Val 170 Leu Gy y Thr Arg Thr 185 Cys 200 Lys Cys Ar g Thr Cys Cys G u 175 Gy <210> 247 <211> 1338 <212> DNA <213> Corynebacter i um gl ut am cum <220> <221> CDS <222> (101)..(1315) <223> RXN02478 <400> 247 gacatcgtcg aagcgctctc cagcggcaac atc cactacgcgc cgtttcgccg catgatttcc aac at t SIle ctt Leu ccc Pr o acc Thr gt c Val gca Al a cgc Ar g acg Thr gac Asp gaa G u ctt Leu ttt Phe ttg Leu ttc Phe gt g Val gca Al a acg Thr gag Gu cgc Ar g gcc Al a gcg Al a gt c Val gat Asp ccc Pr o at t SIle cgc Ar g cga Ar g 45 gaa G u ct g Leu acc Thr ccc Pr o ct c Leu t cc Ser act Thr tgc Cys gac Asp gacgatt atcgcagcgc cgtgctcgct atgctcg atg cgc act agc ct c IVet Arg Thr Ser Leu 1 gcg ctg gtc tgg gat cag ggt Ala Leu Val Trp Asp G n y 15 agt gtt gac gac ctc ccc gca Ser Val Asp Asp Leu Pro Ala tca gac ggc atc acc tgg acc Ser Asp Gy Ile Thr Trp Thr gaa cac cgc ggt gtg ggc gat G u His Arg G y Val G y Asp ttc cac gga ttg tcc aac ctc Phe Hi s G y Leu Ser Asn Leu 80 ctt gaa ccc cgg ctg gcg cgc Leu G u Pro Arg Leu Ala Arg Page 304 NO seql i st cor r ect ed. t xt 90 95 100 Scgg gat gtg agt ggg tgg acg tcg ata agc atg gcc cac tat ttt gcg 451 Arg Asp Val Ser G y Trp Thr Ser I I e Ser 'b t Ala His Tyr Phe Ala ,C 105 110 115 4 gat gtt gat gcc gcg ttc gcc tcg tcg ggg acg gga ctt gtc ctg gcg 499 Asp Val Asp Ala Ala Phe Ala Ser Ser y Thr y Leu Val Leu Ala 120 125 130 gat ggg cgg tgg att cag agt ttt gtg gtg cgg cgc ggg cgc gag att 547 Asp y Arg Trp Ile n Ser Phe Val Val Arg Arg y Arg u Ile C1 135 140 145 00 tcg ctt cgg att ctg cgc agc gat ggc cac atc acc gat att gcc ggc 595 SSer Leu Arg I I e Leu Arg Ser Asp y His I I e Thr Asp I I e Ala y S150 155 160 165 .C ggt aac gaa tcc gcg atg acg cag ctg ccg agc ggt cgg att gtg ctg 643 Gy Asn 3u Ser Ala I'bt Thr n Leu Pro Ser G y Arg Ile Val Leu 170 175 180 cat tcc agg ggg gtg gga cac cgt ctg agc agt gtg tcc gat gat ttc 691 His Ser Arg G y Val G y His Arg Leu Ser Ser Val Ser Asp Asp Phe 185 190 195 ggg gag aca ttc act ccg ctg gag cct gtg cct gaa cta atc gac ccc 739 G y u Thr Phe Thr Pro Leu u Pro Val Pro u Leu Ile Asp Pro 200 205 210 ggc tgc aac ggc cac gtg ttc tac tgg aaa gcg gct gga atg ctc gcc 787 G y Cys Asn G y His Val Phe Tyr Trp Lys Ala Ala G y /b t Leu Ala 215 220 225 gca acg cac ctg gcg gac cct gat ctg cga cgc cac ttg gtg gtt gat 835 Ala Thr His Leu Ala Asp Pro Asp Leu Arg Arg His Leu Val Val Asp 230 235 240 245 tta tcc agc gac gaa gga gcg acc tgg gcg cat cgc atc acc atc gag 883 Leu Ser Ser Asp u G y Ala Thr Trp Ala His Arg Ile Thr Ile u 250 255 260 cgc gaa gaa gcc gcc tat tca acc gct gcg gaa atg ccc aac gga gat 931 Arg u u Ala Ala Tyr Ser Thr Ala Ala u 'b t Pro Asn G y Asp 265 270 275 gtt gcc gtg gtg tgg gaa gca gag gga acg cgc gcg ata aaa tgc acg 979 Val Ala Val Val Trp u Ala u G y Thr Arg Ala ile Lys Cys Thr 280 285 290 gtg atc agc gta aat gat att tcg ctg cgg atc gat gag ccc att tcc 1027 Val Ile Ser Val Asn Asp Ile Ser Leu Arg Ile Asp u Pro Ile Ser 295 300 305 gat gcc ata tcc ctc cgc cat gtg gtg atc aac gat gac cat gac ggc 1075 Asp Ala Ile Ser Leu Arg His Val Val Ile Asn Asp Asp His Asp 3 y 310 315 320 325 atc gaa gtc gca ctg cct gac gca tcg caa tgg ggt gaa ggt gta ttc 1123 Ile u Val Aa Leu Pro Asp Ala Ser n Trp y u y Val Phe 330 335 340 aaa att gtg tcc aat cca gac gcg agc acc caa aaa atc cgc act cga 1171 Lys Ile Val Ser Asn Pro Asp Ala Ser Thr n Lys Ile Arg Thr Arg 345 350 355 ggc aag ccc gcg cga cag acc ctg gaa att ggg gat gaa ttg gtt ttt 1219 G y Lys Pro Ala Arg n Thr Leu u Ile G y Asp u Leu Val Phe Page 305 IND seql i st cor r ect ed. t xt 360 365 370 C- gat atc cgc aag ggt gga gaa gtg gct tac ggc gtc acg gtt cct tat 1267 Asp lie Arg Lys G y G y G u Val Ala Tyr G y Val Thr Val Pro Tyr 375 380 385 4 gat ggt cgc tcg ttg ggg gaa gtt aaa cag gat ttt gga gtg ggg ctg 1315 Asp y Arg Ser Leu G y G u Val Lys G n Asp Phe G y Val G y Leu 390 395 400 405 tagaggccga t t t gcggtcc t t t 1338 0 <210> 248 00 <211> 405 0 <212> PRT S<213> Cor ynebact eri um gl ut arri cum <400> 248 S eIVet Arg Thr Ser Leu I e Ala Arg y Leu Tyr Arg Ile Pro Ala Leu 1 5 10 Val Trp Asp n y Leu Leu Thr Leu Phe Asp Ala Arg Leu Ser Val 25 Asp Asp Leu Pro Ala Pro lle Asp Val Val Ser Ala Arg Ser Ser Asp 40 G y I I e Thr Trp Thr Thr Pro G u Pro Ala IIe Val G u Thr G u His 55 Arg G y Val G y Asp Val Cys Leu Val Thr G y Asp Leu Cys Phe His 70 75 Sy Leu Ser Asn Leu Al a y Phe Phe G u Asp Pro Thr Asp Leu G u 90 Pro Arg Leu Ala Arg Arg Asp Val Ser y Trp Thr Ser Ile Ser IVet 100 105 110 Ala His Tyr Phe Ala Asp Val Asp Ala Ala Phe Ala Ser Ser G y Thr 115 120 125 Sy Leu Val Leu Ala Asp y Arg Trp lie G n Ser Phe Val Val Arg 130 135 140 Arg G y Arg G u lie Ser Leu Arg lie Leu Arg Ser Asp G y His lie 145 150 155 160 Thr Asp lie Ala Gy y Asn u Ser Ala IVet Thr n Leu Pro Ser 165 170 175 G y Arg lie Val Leu His Ser Arg G y Val G y His Arg Leu Ser Ser 180 185 190 Val Ser Asp Asp Phe G y G u Thr Phe Thr Pro Leu G u Pro Val Pro 195 200 205 G u Leu IIe Asp Pro G y Cys Asn G y His Val Phe Tyr Trp Lys Ala 210 215 220 Al a G y Iet Leu Al a Al a Thr Hi s Leu Al a Asp Pro Asp Leu Arg Arg 225 230 235 240 His Leu Val Val Asp Leu Ser Ser Asp G u G y Ala Thr Trp Ala His 245 250 255 Arg lie Thr lie u Arg Gu Gu Ala Ala Tyr Ser Thr Ala Ala u Page 306 vb t A a As p 305 As p G31y Ly s As p ValI 385 Ph e As n 275 Ly s Pr o Hi s G31y Ar g 355 Le u ValI ValI As p Thr Ser G31y 325 Ph e Ar g Ph e Tyr Le u 405 A a I Ie 295 A a (31u I Ie Ly s I Ie 375 G31y seqI i st car r ect ed. t xt 265 Val Val Trp Ou Ala Ou 280 285 Ser Val As n As p I Ie Ser 300 li1e Ser Leu Ar g Hi s Val 315 Val Ala Leu Pr o As p Ala 330 Val Ser As n Pr o As p Ala 345 Pr o Ala Ar g O31n Thr Leu 360 365 Ar g Lys G31y 31y O31u Val 380 Ar g Ser Leu G31y O31u Val 395 270 O31y Le u ValI Ser Ser 350 O31u A a Lys5 Thr Ar g I Ie O31n 335 Thr I Ie Tyr O n <210O> 249 <21 1> 1213 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1213) <223> RXN00343 <400> 249 t t cggt agaa t gggt aggt t gt cgt gct t g agg cacccaagct ct gt tagaaa aaat tgagga agc g at As p agt Ser gt a ValI gga G31y tt c Ph e aac at c I Ie gct A a ggt G31y ag c Ser ac c Thr t cc gt c ValI ggc G31y gcc A a gcg A a ct g Le u t tc ggt G31y ggt G31y acc Thr cgc Ar g caa O31n 75 t t g t cc Ser aag Lys5 ac c Thr aaa Lys5 gcg A a g ac ggc O31y aaa Lys5 t gg Tr p 45 g ac As p gt a ValI gca gct A a gt c ValI t cc Ser gga O31y gt g ValI gcc ;t gt ggt g gat aaccacc tct acaacac agt ct aa at g aaa cac caa t at I'b t Lys Hi s O31n Tyr 1 ggc gga t ta t ca gct gca gt c O3 y 03 y Leu Ser A a A a Val 15 gct gt a at t gaa aag gcc t ca Ala Val Ilie O31u Lys Ala Ser ggc ggt t gg gct t gg act cct O31y O31y Tr p Ala Tr p Thr Pr o gta gtg gaa tcc aaa gaa gaa Val Val O31u Ser Lys O31u O31u ggg gag t ac t ac caa gaa gac O3 y 03 u Tyr Tyr 03 n 03 u Asp 80 cct gaa at g gt c gat t tc tt t Page 307 seqi i st car r ect ed. t xt As n I Ie Se r Ala Ph e L eu As p Ala Ala Pr o 31u l'b t Val1 95 Asp Phe Phe 1 00 g aa O31u at c I Ie cca Pr o ct g Le u 1 50 gct A a cgt Ar g at g vb t ac c Thr gt c ValI 230 ggc G31y gcc A a cgc Ar g ct c Le u at c I Ie 310 cct Pr o c ac Hi s aac As n ggc G31y 120 cca Pr o c ac Hi s cct Pr o t gg Tr p gt g ValI 200 cga Ar g c ac Hi s aaa Ly s gt c ValI g aa O31u 280 cca Pr a gca A a t ca Ser at g vb t g ac As p ct c Le u aac As n ct g Le u ct g Le u 1 70 cat Hi s aaa Ly s gct A a gca A a c aa O31n 250 ct c Le u tt c Ph e g aa O31u tt t Ph e gt c ValI 330 cgc Ar g ct g Le u ccc Pr a gga G31y t at Tyr 155 ac g Thr gcc A a cgc Ar g acc Thr gt g ValI 235 acc Thr gcc A a cca Pr a acc Thr gt c ValI 315 cca Pr a gca A a c ag O31n ggt O31y cgc Ar g 140 g aa O31u aaa Ly s gcc A a aat As n t ct Ser 220 t cg Ser cca Pr a act Thr cgc Ar g ac c Thr 300 aac As n t ac Tyr aaa Ly s t gg Tr p gct A a 125 aaa Ly s ac c Thr tt c Ph e ag g Ar g at g vb t 205 gca A a tt g Le u c ag O31n ggt G31y ac c Thr 285 ggc G31y g ac As p tt c Ph e cca Pr a ac c Thr 110 ggc G31y gt a ValI t cc Ser ct c Le u cgc Ar g 1 90 c ag O31n g ac As p aat As n ggc O31y gga O31y 270 cca Pr a g ac As p ct g Le u aac As n ggc O31y 350 ccc Pr a act Thr ccc Pr a tt c Ph e t ct Ser 1 75 gt c ValI at g vb t aag Ly s t ac Tyr tt g Le u 255 tt c Ph e t ca Ser gga O31y aaa Ly s gga O31y 335 t cc Ser ggc O31y gga O31y aag Ly s ct g Le u 1 60 gct A a at c I Ie gt c ValI ct g Le u aaa Ly s 240 gt a ValI ccc Pr a ggt O31y ct a Le u t cc Ser 320 aaa Ly s at c I Ie gcg A a c ac Hi s agt Ser 145 gga O31y t ca Ser gt g ValI aac As n ggc O31y 225 aac As n g at As p aac As n c aa O31n t cc Ser 305 cca Pr a gt c ValI ggt O31y aaa Ly s cgc Ar g 130 gt t ValI at g vb t c ag O31n c ac Hi s ggt O31y 210 gt t ValI g ac As p tt c Ph e aac As n g aa O31u 290 at g vb t gca A a ggc O31y gt t ValI at c I Ie 115 t cc Ser ct t Le u ggc O31y Ph e at g vb t 1 gca A a g at As p cgc Ar g g aa O31u gt t ValI 275 c ac Hi s gct A a gca A a ac c Thr gt c ValI 355 aac As n gt t ValI cca Pr a at c I Ie g at As p 1 t gg Tr p gca A a ct c Le u gt t ValI gcc A a 260 g ac As p t gg Tr p cgg Ar g t gg Tr p tt c Ph e 340 t cc Ser g ac As p ggg O31y aaa Ly s at g vb t 1 cca Pr a g ac As p ct c Le u ct g Le u ac c Thr 245 act Thr ct g Le u ac c Thr g aa O31u t gc cys 325 ccc Pr a ac a Thr 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 1171 1213 ggt aag cgat t cgt caat gaa gcc aac ggc tac tac gac tac Page 308 seqI i st car r ect ed. t xt G31y Ly s Ar g Ph e ValI As n O31u Ala As n G31y Ty r Ty r As p Ty r 360 365 370 <210> 250 <211> 371 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn <400> 250 I'bt Lys Hi s Le u Tr p O31u Tyr vb t A a Hi s Ser 145 G31y Ser ValI As n G31y 225 As n As p As n O31n A a Tr p Lys5 G n As p I Ie 115 Ser Le u 0Gy Ph e vb t 1 95 A a As p Ar g 0Ou ValI 275 Hi s 0On Tyr Asp Val Ala Val Se r Ala A a Ser Val Leu Thr Pr o G31y Thr 03 u 03 u Phe 03 n 70 G u As p As n I I e Phe Phe 03 u Lys 1 00 As n As p I I e Ty r Val G31y Pr o Lys 135 Pro Lys Leu Arg 150 Ile I'b t A a 03 y 1 65 Asp Pr o Ar g (31y 1 80 Tr p Asp I'b t Val A a Leu Thr A a 215 Leu Leu Val Asn 230 Val Thr 03 y Val 245 A a Thr A a 03 y 260 Asp Leu Arg Lys Tr p Thr Leu Ala 295 I Ie A a G31y 40 Ser Thr Ser As n G31y 120 Pr o Hi s Pr o Tr p ValI 200 Ar g Hi s Lys5 ValI O31u 280 Pr o ValI Tyr 25 G31y Le u Tyr A a Thr 1 05 As n Ph e O31n As p ValI 1 85 Hi s Le u Ser ValI ValI 265 Le u A a ValI 10 O31y A a A a Le u Ph e 90 As p Le u As n Le u Le u 1 70 Hi s Lys5 A a A a O31n 250 Le u Ph e (31u G31y G31y Thr Ar g O31n Le u Le u Pr a O31y Tyr 1 Thr A a Ar g Thr ValI 235 Th r A a Pr a Thr O31y Lys5 Tr p As p ValI A a Tr p A a 125 Ly s Th r Ph e Ar g vb t 205 A a Le u O31n O31y Thr 285 O31y A a ValI Ser O31y ValI A a Thr 110 O31y ValI Ser Le u Ar g 1 O31n As p As n O31y O31y 270 Pr a As p O31y A a O31y ValI O31y Pr a Pr a Thr Pr a Ph e Ser 1 ValI vb t Ly s Tyr Le u 255 Ph e Se r (31y Ser I'bt Ala Arg Ou Ilie Oy Ala O1y Phe Val Asn Asp Leu Lys Ser Page 309 seqI i st car r ect ed. t xt 305 310 315 320 Pr o Ala Ala Tr p Cys Pr o Val Ser Leu Val Pro Tyr Ph e As n G31y Lys 325 330 335 Val G31y Thr Phe Pr o Hi s I Ie NbtAsp Ar g Ala Lys Pro G31y Ser Ilie 340 345 350 G31y Val Val Ser Thr G31y Lys Arg Phe Val Asn (31u Ala Asn G31y Tyr 355 360 365 Tyr Asp Tyr 370 <210> 251 <211> 1083 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> OIDS <222> (1060) <223> RXN01555 <400> 251 ct ggtt t tgt gccgaggat c agccagcaag t taat gat cc t tacat ggcg ct gt tgt tgg cgcggt agt c aat cat gggg gagt at ccca ccgt at ccgc gt g aac aag ggc gt g Val Asn Lys 03 y Val gt g ValI t ac Tyr ac c Thr g ac As p cgc Ar g cgc Ar g g aa O31u aaa Ly s ct g Le u gt a ValI cca Pr o g ac As p aac As n g ac As p gga G31y gt c ValI ac c Thr 120 aaa Ly s ggt G31y ccc Pr o at g vb t g aa O31u t cc Ser at c lIe t ac Tyr act Thr ggc G31y gga G31y acc Thr gcc A a aac As n acc Thr 75 gca A a cac Hi s gt t ValI ct c Le u tt c Ph e cca Pr o ct t Le u gca A a gcg A a gt c ValI cgc Ar g t ca Ser ggc G31y 140 t cc Ser c ag O31n gct A a 45 ct g Le u gt g ValI aac As n tt c Ph e gcc A a 125 at g vb t c ag O31n tt c Ph e gcg A a t gg Tr p t ac Tyr gca A a gt g ValI 110 t gg Tr p at c I Ie aat As n 15 t cc Ser aaa Ly s tt c Ph e cca Pr o gca A a 95 cgc Ar g ct c Le u ac c Thr cca Pr a cgc Ar g gt g ValI cca Pr a c ac Hi s ggc G31y gcc A a at t I Ie at g vb t 1 g aa O31u ac c Thr gga G31y t cg Ser at t I Ie gag O31u at g vb t gt t ValI cca Pr a 145 ct g Le u aac As n gca A a t cc Ser t gg Tr p cgt Ar g t ac Tyr g at As p 130 c ac Hi s cgc Ar g g aa O31u cgc Ar g at c I Ie g ac As p tt c Ph e g aa O31u 115 t cc Ser ct g Le u at g vb t ag c Ser ct a Le u ggc G31y cgt Ar g gt c ValI 1 00 t cc Ser c ac Hi s cct Pr a Page 310 seql i st cor r ect ed. t xt ctc gct ctg caa aaa tac Leu Ala Leu G n Lys Tyr 150 155 tcc ttg gat gaa ttg gca Ser Leu Asp u Leu Al a 170 gaa caa acc gtc aaa cgc G u G n Thr Val Lys Arg 185 gaa gac ttc cac aag ggc G u Asp Phe His Lys G y 200 cca gac aac aag cca aac Pro Asp Asn Lys Pro Asn 215 ttc tac gca atc gct gta Phe Tyr Ala lIe Ala Val 230 235 atc agc atc aac ccc aac Ile Ser Ile Asn Pro Asn 250 atc att gga ctg tac tcc Ile Ile G y Leu Tyr Ser 265 tct gaa tat cct ggt gct Ser G u Tyr Pro Gy Al a 280 ggt tgg atc gca gca cag Sy Trp Ile Ala Ala n 295 gga gct aag gca gga tat G y Al a Lys Al a G y Tyr 310 315 tac <210> 252 <211> 320 <212> PRT <213> Corynebacter i um gl <400> 252 Val Asn Lys y Val Val 1 5 G u Leu Arg IVet Lys Tyr Thr Asn G u Ser Ala Thr G y Ala Arg Leu G y Asp Ser Ser Ile y Thr Arg 70 atc gac tcc gga I e Asp Ser G y cgc Ar g t ac Tyr gaa G u ccc Pr o 220 gt c Val gga Gy gca Al a ggt Gy cac Hi s 300 agc Ser aat Asn ct c Leu 205 aac Asn cca Pr o cag G n gga Gy t ca Ser 285 gcg Al a at t SIle acc Thr 190 ct c Leu gt c Val acc Thr gtt Val aat Asn 270 cag G n gt g Val t ac ctg cac Tyr Leu Hi s 160 gtg gac gct Val Asp Al a gct aaa acg Ala Lys Thr ggt caa gcc G y G n Al a 210 cca atc aag Pro Ile Lys 225 ct g gcc act Leu Al a Thr 240 agt gaa gat Ser G u Asp gcc caa t ct Al a G n Ser ggt tcc gga G y Ser G y 290 aaa gcg gga Lys Al a G y 305 aag taat t gcl Lys 320 cgc Ar g ggt Gy 195 gcc Al a aag Lys gcc Al a ggg Gy gt c Val 275 at g Met aaa Lys gca gga t ca Al a G y Ser ggc Gy 180 at c SIle ggc Gy gga Gy ttt Phe gag Gu 260 at g Met acc Thr t cc Ser 165 ct g Leu gac Asp gat Asp ccg Pr o ggc Gy 245 ccc Pr o gct Al a ttt Phe gga Gy 595 643 691 739 787 835 883 931 979 1027 gcc gcg tct tct Ala Ala Ser Ser ttg gtgggttgct 1080 1083 ut ari cum Leu Val Al a bet Pro G u 25 G y Asp Thr 40 Asp Asn G y 55 Al a Asp G y Gy Gy Phe Ser On Asn Pro 10 Pro Thr Pro G n Phe Ser Arg IVet Al a Leu Al a Al a Lys Val G u Asn Ala Leu Trp Phe Pro Ser Thr Ala Val Tyr Pro His 75 Page 311 I Ie Tr p Asp Ar g G31y Ar g seqI i st car r ect ed. t xt Leu Gy Val Ilie Ala Val Asn Al a Al a 03 y O31u vb t ValI Pr o 145 Le u As p Ly s O31n I Ie 225 Al a O31u O31n Ser Al a 305 Ph e O31u 115 Ser Le u Al a Ar g G31y 1 95 Al a Ly s Al a G31y ValI 275 vb t As p As n Thr Ly s Ser 1 65 Le u As p As p Pr o G31y 245 Pr o Al a Ph e Ser Thr Al a 135 Al a Le u O31n As p As p 215 Tyr Ser I Ie (31u Tr p 295 Ser 1 Pr o Tyr O31n O31u ValI 1 Hi s Ly s I Ie As n Le u 265 Pr a Al a Tyr Thr O31y Ly s Le u 1 70 Ly s Ly s Pr a Al a Pr a 250 Tyr O31y Al a Hi s ValI Le u Tyr 1 Al a Ar g O31y As n ValI 235 As n Ser Al a O31n Tyr 315 Ph e Al a 125 vb t As p Ser As n Le u 205 As n Pr a O31n O31y Ser 285 Al a Ar g Le u Thr O31y O31y 1 Ph e Ph e O31y Pr a ValI 255 As p ValI (31y 03 y Lys Ser 03 y Al a Lys Al a 0 y Al a Al a Se r Se r <210O> 253 <21 1> 1428 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (1405) <223> RXN01166 <400> 253 accgtaccca cagacacacc agaattaaca gaaacagact gaaaaacaac atcgctcgac at gcgcgt aa t cct aacccg cgcacact aa t gt ggccgat at g ggc t ac acc aac 115 I'b t O31y Ty r Th r As n 1 ctc aac gac aca cgg gtc ttg cgc gcc ggg tca t gt gat gcc t gg t gg 163 Leu Asn Asp Thr Arg Val Leu Arg Al a 0 y Ser Cys Asp Al aTrp Trp Page 312 seql i st cor r ect ed. t xt cgc acg atg Arg Thr IVet cgc atc at g Arg lie Iet ccg cta gtg cag Pro Leu Val G n gga agt gag gca G y Ser G u Al a cag Sn gtc ttt cgg Val Phe Arg ggc ttt gac G y Phe Asp ggt ctc tcg cgg Sy Leu Ser Arg cct gat cgg aaa Pro Asp Arg Lys gat gtc Asp Val cca cat ttc ggc Pro His Phe G y gca gct gt t Al a Al a Val 60 cga gt t ccc Arg Val Pro ggt cta aaa cac G y Leu Lys Hi s ggc Gy acg ttg gtc aat Thr Leu Val Asn gct Al a gca ccc ttg aaa Ala Pro Leu Lys ttg ggc gca cgg Leu G y Ala Arg gag ccc aac ccc G u Pro Asn Pro gcg Al a agt tcg tac cgt Ser Ser Tyr Arg gaa tac atc acc Su Tyr Ile Thr ggt gat Sy Asp 100 t cc gca ggt Ser Al a G y cgc ccc tgg Arg Pro Tr p 120 gcc atc act gcg Ala lie Thr Ala ggc gct gtc ctc Gy Ala Val Leu ttt tcc aca Phe Ser Thr 115 gct cca t cc Ala Pro Ser aca acc ggc ccg Thr Thr G y Pro ccc gcg atc gcc Pro Ala lie Ala acc caa Thr G n 135 ggc gtc gca cag Sy Val Ala n cac Hi s 140 tgc gat ccc tcc Cys Asp Pro Ser cac Hi s 145 acc tgc gcc atc Thr Cys Ala lle gga Gy 150 ctc aac gca ttc Leu Asn Ala Phe gac aaa ccc ttc Asp Lys Pro Phe gca atc att gct Ala lie Ile Ala gaa ctc ccc gt c G u Leu Pro Val at c Ile 170 ctc tgg ttt cta Leu Trp Phe Leu gct Al a 175 cac gga ctt gac Hi s G y Leu Asp gtt gtg Val Val 180 ttc atc gat Phe IIe Asp gat tcc atc Asp Ser Ile 200 ccc cgc gac ccc Pro Arg Asp Pro gca Al a 190 acc ggc gtc caa Thr y Val G n tac tat tgc Tyr Tyr Cys 195 ct c gcc t cc Leu Al a Ser gct gca gct aaa Ala Ala Al a Lys ctt ctc gac gcc Leu Leu Asp Ala gt c Val 210 aga caa Arg G n 215 ctc ggc ctt tca Leu G y Leu Ser ccg Pr o 220 gaa gca ccg ctt G u Ala Pro Leu ggc Gy 225 ctg tgg gga ttc Leu Trp Gy Phe tcc Ser 230 caa gga ggc ggc Gn y G y y gcc Al a 235 act ggc tgg gct Thr Gy Trp Ala gca Al a 240 caa ttg cag gat G n Leu G n Asp gca cct gat gtc Ala Pro Asp Val cgc Ar g 250 cca aag gca gcg Pro Lys Ala Ala gtg ggc gct cca Val Gy Ala Pro cca gtg Pro Val 260 gat ctc ttc Asp Leu Phe gtc ttg gac act Val Leu Asp Thr gt c Val 270 gac ggc gga ttg Asp G y G y Leu ct c acc gga Leu Thr G y 275 gtg att gcc tac gcc atc gcg gga ctt Val Ile Ala Tyr Ala Ile Ala Gy Leu gca gtg aac tct tca gag atg Ala Val Asn Ser Ser G u bet Page 313 seql i st cor r ect ed. t xt ttt Phe ct g Leu 310 t ac Tyr at t SIle ct g Leu aat Asn tgg Tr p 390 cgt Ar g aac Asn 280 gaa G u aat Asn tct Ser gac Asp cgt Ar g 360 gat Asp gac Asp cca Pr o gaa G u atg tcg Vet Ser acc agc Thr Ser 315 cgc ggg Arg G y 330 ctg cca Leu Pro gcg cca Al a Pr o att ccc Ile Pro ggt acg Sy Thr 395 cgc aca Arg Thr 410 tac tcg Tyr Ser 285 gtg tta aat gaa cgc Val Leu Asn G u Arg 300 tgc gcg gga ggt tcc Cys Al a G y y Ser 320 tgg aca cat cag ggc Trp Thr His G n G y 335 ctt gtt gtc gct gag Leu Val Val Ala G u 350 gaa atc cca gtg ct g G u lie Pro Val Leu 365 att gat ccc att agg Ile Asp Pro Ile Arg 380 cca ttg acc tgg cat Pro Leu Thr Trp His 400 ggt ctc aac cat ttc G y Leu Asn Hi s Phe 415 gga tgg ctc ata gat Sy Trp Leu Ile Asp 430 gga Gy 305 ttg Leu acg Thr ttt Phe ttg Leu gaa G u 385 gaa ggu ggg Gy 290 gt c Val ttg Leu ccg Pr o ggg Gy tgg Tr p 370 ttg Leu t cc Ser ccc Pr o agt Ser gcc Al a ct g Leu aag Lys 355 ggc Gy cgt Ar g caa G n t at Tyr gat Asp agt Ser gca Al a 340 caa G n tct Ser gat Asp gcg Al a ttt Phe 420 gt g Val ggc Gy 325 gac Asp aag Lys aaa Lys agc Ser ccg Pr o 405 aga Ar g 1027 1075 1123 1171 1219 1267 1315 1363 1405 1428 cat ctt gtc Hi s Leu Val 435 tgagtgccgt tttaaaggct cgg <210> 254 <211> 435 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 254 IVet y Tyr Thr Asn Leu Asn Asp Thr 1 5 Cys Asp Ala Trp Trp Arg Thr IVet Ser 25 Su Ala Val Phe Arg Arg Ile 'e t y 40 Lys Pro y Phe Asp Asp Val Pro His 55 Pro G y Leu Lys His G y Thr Leu Val 70 Leu Gy Ala Arg Gy G u Pro Asn Pro Tyr lie Thr y Asp Ser Ala y Arg 100 105 Arg Val Leu 10 Pro Leu Val Leu Ser Arg Phe G y Al a Asn Al a Al a Al a Ser Ser 90 Al a II e Thr Page 314 Ar g G n Ar g Al a Pr o Tyr Al a Al a G n Pr o Val Leu Ar g Thr 110 Sy Sy Asp Ar g Lys Phe Sy S Val Leu Phe Ser Thr Arg Pro Trp 115 120 eqi i st car r ect ed. t xt Th r Th r G31y Pro Ar 12 g Pr o Ala I Ie A a Hi s 145 A a G31y ValI A a G31y 225 O31n G31y G31y As n G31y 305 Le u Thr Ph e Le u O31u 385 O31u G31y Hi s A a CYS I Ie As p Tyr 1 95 Le u Tr p O31n Pr o Le u 275 Ser Ser A a Le u Ly s 355 G31y Ar g O31n Tyr ValI 435 Ser I Ie Tyr 1 65 ValI CYS Ser Ph e Tyr 245 ValI G31y vb t ValI G31y 325 As p Ly s Ly s Ser Pr o 405 Ar g Thr G31y 150 O31u Ph e As p Ar g Ser 230 A a As p ValI Ph e Le u 310 Tyr I Ie Le u As n Tr p 390 Ar g As n O31n 135 Le u Le u I Ie Ser O31n 215 O31n Pr o Le u I Ie O31u 295 Ly s Ser Le u O31y As p 375 A a ValI Le u O31y As n Pr o As p I Ie 200 Le u O31y As p Ph e A a 280 O31u As n Ser As p Ar g 360 As p As p Pr a O31u ValI A a ValI Tyr 1 85 A a O31y O31y ValI Ar g 265 Tyr I Ie I Ie Ser As p 345 ValI ValI Ly s O31y Ly s 425 A a Ph e I Ie 1 70 Pr a A a Le u O31y Ar g 250 ValI A a vb t Thr Ar g 330 Le u A a I Ie O31y Ar g 410 Tyr O31n Tyr 1 Le u Ar g A a Ser A a 235 Pr a Le u I Ie Ser Ser 315 O31y Pr a Pr a Pr a Thr 395 Thr Ser Hi s 140 As p Tr p As p Ly s Pr a 220 Thr Ly s As p A a ValI 300 CYS Tr p Le u O31u I Ie 380 Pr a O31y O31y CYS Ly s Ph e Pr a Ser 205 O31u O31y A a Thr O31y 285 Le u A a Thr ValI I Ie 365 As p Le u Le u Tr p As p Pr a Le u A a 1 Le u A a Tr p A a ValI 270 Le u As n O31y Hi s ValI 350 Pr a Pr a Thr As n Le u 430 Pr a Ph e A a 1 Thr Le u Pr a A a ValI 255 As p A a O31u O31y O31n 335 A a ValI I Ie Tr p Hi s 415 li1e <210O> 255 <21 1> 1281 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn Page 315 seql i st cor r ect ed. t xt <220> <221> <222> <223> CDS (101)..(1258) RXN02001 <400> 255 gcggtttcgt catggataag gactgtgttc gggaccattg gatagtccag catagaccgt gctttatcga aggtgaaccc cgatactcgt gtcaaaaggc at g Met 1 ccc gtt atc Pro Val lie agt atc gcc agt Ser Ile Ala Ser tcc gac gag atg Ser Asp G u IVet cgc tgg cgg cgt Arg Trp Arg Arg cac ct g Hi s Leu cat caa aac His G n Asn gtg gcc gag Val Ala G u gaa atc agc ttt G u lie Ser Phe gat Asp tgt gtg gaa act Cys Val G u Thr gcg gcc ttc Al a Al a Phe cac acc ggc Hi s Thr G y cag ctg cgc agc G n Leu Arg Ser ggg gtg gat gaa G y Val Asp G u atc gcg I e Al a aaa acc ggt atc Lys Thr G y Ile at c Ile gcc ctg att cac Ala Leu Ile Hi s cgc gag gct ggc Arg G u Ala G y ccc Pr o gtc gtc ggc ctg Val Val G y Leu gcc gat atg gac Ala Asp Ibet Asp gcg Al a ctg ccg ctg acc Leu Pro Leu Thr att acc ggc gtc I e Thr G y Val gac Asp tat gcc tcg acc Tyr Ala Ser Thr ccc gga aaa at g Pro y Lys IVet cac gcc Hi s Al a 100 tgc ggc cac gac ggc cac acg acc atg ctg ctg ggc gcc gcc aaa tat Cys G y His Asp G y His Thr Thr IVet Leu Leu G y Ala Ala Lys Tyr 105 110 115 ctg gcc gag Leu Al a G u 120 acg cgc aat ttc Thr Arg Asn Phe gca ggt acc gtc gcg ctg atc ttc cag Ala y Thr Val Ala Leu Ile Phe G n 125 130 cct gcg Pro Ala 135 gaa gaa aac ggc G u G u Asn G y ggc SGy 140 ggc gcg ggc gtt G y Al a G y Val gtc gat gaa ggc Val Asp G u Gy gt c Val 150 ctc gac cgc ttt Leu Asp Arg Phe atc gcc gaa gt c i e Al a G u Val t ac Tyr 160 gcc ctg cac aac Ala Leu His Asn ccc ggc ctg ccg Pro G y Leu Pr o ctt Leu 170 ggc cat ttt atg G y His Phe Met aca gcc ggc ccg Thr Ala G y Pro at c at g Ile bet 180 gcc gct gtc Al a Al a Val ggt gcc aaa G y Al a Lys 200 acg ttc gac atc Thr Phe Asp Ile aac Asn 190 att acc gga cgc lie Thr G y Ar g ggc ggc cac G y G y Hi s 195 gcc gtc gga Ala Val G y ccg cac caa acc Pro His G n Thr gac ccc atc gtc Asp Pro lie Val gca Al a 210 att gtc lie Val 215 caa gcg ttt caa G n Al a Phe G n acg Thr 220 ata gtc agc cgg lie Val Ser Arg cac aat ccg gtc His Asn Pro Val Page 316 seql i st cor r ect ed. t xt gag gac ctt gtc gtg tcg gtc G u Asp Leu Val Val Ser Val 230 235 aat atc atc ccc gaa acc gcc Asn lie lie Pro Gu Thr Ala 250 aac aaa gac gtg cag gcc atg Asn Lys Asp Val G n Al a Iet 265 gcg ggc caa gct gca gcc tat Al a y G n Ala Ala Al a Tyr 280 cgc aac tat ccc gcc acc att Arg Asn Tyr Pro Ala Thr Ile 295 300 gcc gaa gtc gcg ggc gag gtc Ala Gu Val Ala Gy Gu Val 310 315 cgc ggg atg ggg gcc gag gat Arg G y Iet y Al a u Asp 330 ggt gcc tac ctg ttc gtc ggt Sy Ala Tyr Leu Phe Val y 345 ccc gcc tat aat ttc aac gac Pro Ala Tyr Asn Phe Asn Asp 360 ttg gcc cgc atg gca gaa cgc Leu Ala Arg IVet Ala G u Arg 375 380 cgct cgaaga t gc <210> 256 <211> 386 <212> PRT <213> Cor ynebact er i um gl ut a <400> 256 IVet Pro Val Ile Asn Ser Ile 1 5 Trp Arg Arg His Leu His n G u Thr Ala Ala Phe Val Ala G u IIe His Thr G y IIe Al a 55 G y Arg Gu Ala G y Pro Val 70 Leu Pro Leu Thr Gu Ile Thr acg caa at c Thr G n Ile tat atc aac Tyr Ile Asn 255 gt c at c acg Val Ile Thr 270 ggg gtc gag G y Val G u 285 aac gac gcc Asn Asp Al a ggc ctc ggg G y Leu G y ttc tcg tat Phe Ser Tyr 335 aat ggc gac Asn G y Asp 350 gag gcc gcg G u Ala Al a 365 cac Hi s 240 ggc G y cgg Ar g gcg Al a gcc Al a gt c Val 320 ttc Phe agc Ser ccc Pr o acc ggc agc Thr G y Ser act gt c cgc Thr Val Ar g at gaa gaa bt G u G u 275 acg ctg acc Thr Leu Thr 290 aaa gcc gcc Lys Al a Al a 305 aac ccg aac Asn Pro Asn ctc gaa aag Leu G u Lys gcg ggc ctt Al a G y Leu 355 t ac ggc gca Tyr G y Al a 370 gcc Al a acc Thr 260 at c SIe t ac Tyr at c SIe ggc G y cgc Ar g 340 cac Hi s tcg Ser 835 883 931 979 1027 1075 1123 1171 1219 1268 1281 ccc ttg ccg tta Pro Leu Pro Leu aag ggc Lys G y 385 t gat ccat gg ri cum Al a Ser Asn Pro G u G n 40 Lys Thr Val G y G y Val Phe Ser Asp 10 G u i e Ser Leu Arg Ser G y lie lle Leu Arg Ala Asp Tyr Al a 90 Page 317 G u Phe Phe Al a Asp Ser Thr Cys Val SIe Asp Thr G31y Lys l'bt Hi s Ala Cys (31y Hi 1 00 seqi i st car r ect ed. t xt s As p G3 y Hi s Th r Th r 1 05 l'bt Leu Leu 110 a3 y A A a b [vb t V, 145 A a b A a G3 G3 y A Val A 2 As n Hi 225 Thr a3 Thr V, Thr L Lys A 305 As n Pi Leu a3 A a a3 Ty r a3 3-' Lys a3 385 <210O> <211 <21 2> <21 3> <220> <221 <222> <223> A a 115 li1e As p Hi s Pr o a31y 1 95 A a As n Ser Ar g a31u 275 Thr A a As n Lys5 Le u 355 A a Tyr a31n a31y a31n 1 65 vb t Hi s a31y ValI As p 245 Ph e ValI As n A a Ser 325 Pr o As n Ph e Le u Pr o ValI 150 Pr o A a ay le au 230 As n As n A a Ar g A a 310 Ar g a31y Pr a Le u A a A a 135 Le u a31y A a A a ValI 215 As p I Ie Lys5 a31y As n 295 a31u a31y A a A a A a 375 Thr a31u Ar g Pr a As p 1 85 Pr a A a ValI Pr a ValI 265 A a Pr a A a a31y Le u 345 As n b t Ar g As n Ph e Le u 1 70 Thr Hi s Ph e ValI a31u 250 a31n A a A a a31y A a 330 Ph e Ph e A a As n a31y A a 1 55 a31y Ph e a31n a31n Ser 235 Thr A a A a Thr a31u 315 a31u ValI As n a u A a 125 a31y A a Ph e I Ie Ar g 205 li1e Thr Tyr ValI a31y 285 As n a31y Ph e As n a31u 365 Pr a a31y A a a31u vb t As n 1 As p ValI a31n I Ie I Ie 270 ValI As p Le u Ser a31y 350 A a Le u Thr a31y ValI Thr 1 li1e Pr a Ser I Ie As n 255 Thr a31u A a a31y Tyr 335 As p A a Pr a ValI ValI Tyr 1 Thr Thr I Ie Ar g Hi s 240 a31y Ar g A a A a ValI 320 Ph e Ser Pr a Le u 257 570 DNA Cor ynebact er i urn gi ut ani cumn ODS 101). (547) RXN031 Page 318 I0 2seql i st cor r ect ed. t xt <400> 257 agaggtgttt aaattcccgc ctctgcccca gatttaaaac tatgatgagg gatagcttct Scaattttcca ggttttccaa aatgaaagag gttcacgcac atg cct act tat act 115 'bt Pro Thr Tyr Thr 1 tgt tgg tcg caa aga att cgc att tct agg gaa gcc aag caa cgc atc 163 i Cys Trp Ser Gn Arg Ile Arg Ile Ser Arg u Ala Lys Gn Arg lle 15 gct gag gca atc acc gat gcc cac cat gaa tta gcg cat gct ccc aag 211 CAl Ala u Ala Ile Thr Asp Ala His His u Leu Ala His Ala Pro Lys 30 00 Stat ttg gtg cag gtg att ttc aat gag gtg gag cct gat tct tat ttc 259 0 Tyr Leu Val G n Val Ile Phe Asn G u Val G u Pro Asp Ser Tyr Phe C1 40 45 IO 0 att gcg gcg cag tcg gcg tcg gaa aac cac att tgg gtc caa gca acg 307 0 Ile Ala Ala G n Ser Al a Ser G u Asn His Ile Trp Val G n Ala Thr C(1 55 60 att cgt tcg ggg cgt aca gag aag caa aaa gag gaa ctt ctg ctt cgg 355 lie Arg Ser y Arg Thr G u Lys G n Lys G u G u Leu Leu Leu Arg 75 80 ctg aca caa gag atc gcg ctg att ctt ggg atc ccc aat gaa gaa gta 403 Leu Thr Gn G u lie Al a Leu Ile Leu G y lie Pro Asn G u G u Val 95 100 tgg gta tat ata ccg gag att cct ggt tcc aat atg acg gaa tat ggc 451 Trp Val Tyr lie Pro u lie Pro G y Ser Asn Iet Thr G u Tyr y 105 110 115 cgt ctc ctc atg gaa cct ggc gaa gag gag aag tgg ttt aat tcg ctt 499 Arg Leu Leu t G u Pro Gy G u G u G u Lys Trp Phe Asn Ser Leu 120 125 130 ccc gaa ggc ctg cgg gaa agg ttg acc gag cta gaa gga tcg tca gaa 547 Pro G u y Leu Arg G u Arg Leu Thr G u Leu G u G y Ser Ser G u 135 140 145 t agct ct cga ataggccatt t ct 570 <210> 258 <211> 149 <212> PRT <213> Corynebacter i um gl utam cum <400> 258 IVet Pro Thr Tyr Thr Cys Trp Ser G n Arg lie Arg lie Ser Arg G u 1 5 10 Ala Lys Gn Arg lie Al a u Ala lie Thr Asp Ala His His u Leu 25 Ala His Ala Pro Lys Tyr Leu Val G n Val lie Phe Asn u Val u 40 Pro Asp Ser Tyr Phe Ile Ala Ala G n Ser Ala Ser u Asn His lie 55 Trp Val G n Ala Thr I e Arg Ser y Arg Thr G u Lys G n Lys G u 70 75 Su Leu Leu Leu Arg Leu Thr n u Ile Ala Leu Ile Leu Gy Ile Page 319 seqI i st car r ect ed. t xt 90 Pro Asn Ou Ou Val Tr p Val Ty r Ilie Pro Ou Ilie Pro G1y Ser Asn 100 105 110 N/t Thr G31u Ty r G31y Arg Leu Leu N/t (31u Pr o 31y G31u 31u G31u Lys 115 120 125 Tr p Phe As n Ser Leu Pr o 31u G31y Leu Ar g (31u Ar g Leu Thr (31u Leu 130 135 140 G3 u G3 y Ser Ser G3 u 145 <210O> 259 <21 1> 1083 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1060) <223> RXN01466 <400> 259 aat ccat gat cccaaact ac ct caaagcgc t tgt aggct a agact tat gg at acacaacg cggctcattg cggggaaaag ctcataaagc aaggctaaag cgc Ar g ac c Th r ggc gca A a t ac Ty r gt g ValI gag O31u c ac Hi s ggc G31y c aa O31n ggt G31y ag c Ser gt c ValI c ag O31n at c I Ie t gc cys c ac Hi s 120 ct c Le u ct c Le u aaa Ly s c ac Hi s tt c Ph e g ac As p gcc A a at c I Ie ccg Pr o gca A a gag O31u gt c ValI t ac Ty r c ac Hi s ct a Le u ggc G31y gga G31y ggc G31y aac As n 140 ggC G31y cgc Ar g gt t ValI tt c Ph e ac c Th r ggc G31y gcg A a 110 ct c Le u gt t ValI gca A a 15 c ac Hi s ggC G31y ac c Th r tt t Ph e gcc A a 95 aac As n at t I Ie aaa Ly s gca A a ac c Th r cat Hi s ct c Le u t ac Ty r 80 ac c Th r g at As p tt g Le u c99 Ar g at g vb t 1 tt t Ph e at c I Ie gcc A a gcc A a cca Pr o gt t ValI gt t ValI gca A a 145 acg cca aat ggt Thr Pr o As n G31y agc aaa aac cgt Ser Lys Asn Arg gt g gac t cc gac Val Asp Ser Asp gaa aat gcc acg 03 u Asn A a Thr gcc gaa gt g t at Ala O31u Val Tyr aat at t aaa agc Asn Il e Lys Ser cag at c cgc cca O31n Ilie Ar g Pr o 1 00 ct C gca gcc at c Leu A a A a Il e 115 ggg cat t cc ct c G31y Hi s Ser Leu 130 gat cac t ca ct t Asp Hi s Ser Leu Page 320 S cgg aag agg atc gtc ggc atg gtt Arg Lys Arg Ile Val Gy IVbt Val 150 155 eql i st cor r ect ed. t xt cta at c acc aca tc Leu Val Al a Thr Se g atc gaa t ca r Ile Gu Ser tta Leu aac Asn cgc Ar g ttc Phe at c SIle 230 caa G n ggc Gy gcc Al a gaa G u acc Thr aaa Lys t ac Tyr 200 cga Ar g gaa G u cac Hi s gt c Val cgc Ar g 280 gca Al a ggt Gy 170 gcc Al a acc Thr aca Thr ccc Pr o gaa Gu 250 gcc Al a tgc Cys cat Hi s ct a Leu gt c Val aca Thr aac Asn tt g Leu 235 ct c Leu ggc Gy gaa G u at g Met cca Pr o gaa G u ttt Phe gat Asp 220 gat Asp gat Asp gaa G u gt c Val ctt Leu 300 caa G n gca Al a ct a Leu 205 gaa G u acc Thr gcc Al a tta Leu tgg Tr p 285 ccg Pr o gt c Val gcc Al a 190 gcc Al a gt c Val ttc Phe gca Al a gat Asp 270 ccc Pr o ctt Leu ct g Leu 175 ccc Pr o ccc Pr o at c SIle gt c Val cca Pr o 255 gat Asp ggc Gy gaa G u 160 gca Al a aac Asn acc Thr gat Asp ggt Gy 240 gca Al a gt c Val gca Al a gcg Al a ggc Gy 320 t ca Ser gat Asp ct g Leu ttc Phe 225 ttc Phe ttg Leu acc Thr cgc Ar g cca Pr o 305 ccc Pr o gcc Al a gcc Al a 210 cac Hi s ttc Phe gaa Gu cca Pr o ctt Leu 290 gga Gy ctt Leu caa G n 195 acc Thr gcc Al a gac Asp ggc Gy at t SIle 275 caa G n at c SIle gcc Al a 180 aaa Lys gca Al a gcc Al a gac Asp ct c Leu 260 agc Ser at c SIle ct c Leu 165 gac Asp ttc Phe gt c Val at g Met ct c Leu 245 aaa Lys caa G n gca Al a aat Asn 595 643 691 739 787 835 883 931 979 1027 aat gcg atc ggc aac att ttg gac ggg ctg Asn Ala lle y Asn Ile Leu Asp y Leu 310 315 tgg <210> 260 <211> 320 <212> PRT <213> Corynebacter i um gl ut am cum <400> 260 IVet Thr Pro Asn Gy Arg Arg n Leu Leu 1 5 10 Phe Ser Lys Asn Arg Thr Pro y Leu Lys 25 lie Val Asp Ser Asp y Leu Ser lie His 40 Ala G u Asn Ala Thr Al a Thr Val Val Phe 55 Ala Ala G u Val Tyr Tyr Met G n Val Asp 70 Pag tgaggaacct ggttcgggcg 1080 1083 Leu Hi s Thr SIle Tyr 75 e 321 G u Arg G y Ala Ala Val Asp Arg His Thr Tyr bet Val G y His His G y Phe Thr Leu Leu G n Thr Phe Tyr seqI i st car r ect ed. t xt Pro Asn Ilie Lys Ser Val Leu IlieAsp Ala Arg Gy Hi 90 s 03 y Al a Th r G31y ValI ValI Al a 145 Thr Ser As p Le u Ph e 225 Ph e Le u Thr Ar g Pr o 305 I Ie Al a 115 Hi s Hi s I Ie Le u O31n 1 95 Thr Al a As p Oy le 275 O31n I Ie Pr o I Ie Le u Le u Ser 1 65 As p Ph e ValI vb t Le u 245 Ly s O31n Al a As n (31u Hi s G31y Ar g 150 Le u As n Ar g Ph e I Ie 230 O31n O31y Al a O31u As n 310 Le u O31u O31y 135 Ly s Ser I Ie O31n O31n 215 Hi s O31u Tyr As p O31y 295 Al a cys Hi s 120 Le u Ar g Thr Ly s Tyr 200 Ar g O31u Hi s ValI Ar g 280 Al a I Ie Thr 1 05 Al a Thr I Ie O31n As n 1 85 Al a As p Thr As p Le u 265 li1e O31y O31y O31u Thr Le u O31y 1 55 Le u ValI Thr As n Le u 235 Le u O31y O31u vb t I Ie 315 Thr Pr o 125 Le u ValI O31n Al a Le u 205 O31u Thr Al a Le u Tr p 285 Pr o As p Al a 110 Le u ValI Le u ValI Al a 1 Al a ValI Ph e Al a As p 270 Pr a Le u O31y As n I Ie Ly s ValI Le u 1 Pr a Pr a I Ie ValI Pr a 255 As p O31y O31u Le u As p Le u Ar g Al a 1 Al a As n Thr As p O31y 240 Al a ValI Al a Al a O31y 320 <210O> 261 <21 1> 1137 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1114) <223> RXN01145 <400> 261 t aat gt agt t gt ct gcccaa gcgagt t aaa ct cccacgat t tacagt ggg gggcagacat ctt t tcacca aaat tt t tac gaaaggcgag att t tct ccc at g gct at t gaa ct g 115 I'b t Al a Ii e 0u Leu 1 ctt tat gat gct gac gct gac ctc tcc ttg atc cag ggc cgt aag gt t 163 Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ilie O31n 31y Ar g Lys Val Page 322 IO seql i st cor r ect ed. t xt 15 Sgcc atc gtt ggc tac ggc tcc cag ggc cac gca cac tcc cag aac ctc 211 Ala IIe Val G y Tyr G y Ser n G y His Ala His Ser G n Asn Leu 30 d) 4 cgc gat tct ggc gtt gag gtt gtc att ggt ctg cgc gag ggc tcc aag 259 Arg Asp Ser Gy Val G u Val Val Ile Gy Leu Arg G u Gy Ser Lys 40 45 tcc gca gag aag gca aag gaa gca ggc ttc gag gtc aag acc acc gct 307 Ser Ala G u Lys Ala Lys G u Ala y Phe G u Val Lys Thr Thr Ala C(1 55 60 00 gag gct gca gct tgg gct gac gtc atc atg ctc ctg gct cca gac acc 355 G u Ala Ala Ala Trp Al a Asp Val Ile IVet Leu Leu Ala Pro Asp Thr S 70 75 80 (N tcc cag gca gaa atc ttc acc aac gac atc gag cca aac ctg aac gca 403 0 Ser n Ala u Ile Phe Thr Asn Asp Ile u Pro Asn Leu Asn Ala 95 100 (N ggc gac gca ctg ctg ttc ggc cac ggc ctg aac att cac ttc gac ctg 451 G y Asp Al a Leu Leu Phe G y Hi s G y Leu Asn lie Hi s Phe Asp Leu 105 110 115 atc aag cca gct gac gac atc atc gtt ggc atg gtt gcg cca aag ggc 499 Ile Lys Pro Ala Asp Asp Ile Ile Val y IVet Val Ala Pro Lys y 120 125 130 cca ggc cac ttg gtt cgc cgt cag ttc gtt gat ggc aag ggt gtt cct 547 Pro G y His Leu Val Arg Arg G n Phe Val Asp G y Lys G y Val Pro 135 140 145 tgc ctc atc gca gtc gac cag gac cca acc gga acc gca cag gct ctg 595 Cys Leu lie Ala Val Asp G n Asp Pro Thr y Thr Ala G n Ala Leu 150 155 160 165 acc ctg tcc tac gca gca gca atc ggt ggc gca cgc gca ggc gtt atc 643 Thr Leu Ser Tyr Ala Ala Ala lie y y Ala Arg Ala y Val lie 170 175 180 cca acc acc ttc gaa gct gag acc gtc acc gac ctc ttc ggc gag cag 691 Pro Thr Thr Phe G u Al a u Thr Val Thr Asp Leu Phe y G u G n 185 190 195 gct gtt ctc tgc ggt ggc acc gag gaa ctg gtc aag gtt ggc ttc gag 739 Ala Val Leu Cys y G y Thr G u G u Leu Val Lys Val y Phe G u 200 205 210 gtt ctc acc gaa gct ggc tac gag cca gag atg gca tac ttc gag gtt 787 Val Leu Thr G u Al a y Tyr G u Pro G u Met Al a Tyr Phe G u Val 215 220 225 ctt cac gag ctc aag ctc atc gtt gac ctc atg ttc gaa ggt ggc atc 835 Leu His G u Leu Lys Leu lie Val Asp Leu IVet Phe G u G y G y lie 230 235 240 245 agc aac atg aac tac tct gtt tct gac acc gct gag ttc ggt ggc tac 883 Ser Asn IVet Asn Tyr Ser Val Ser Asp Thr Ala G u Phe y y Tyr 250 255 260 ctc tcc ggc cca cgc gtc atc gat gca gac acc aag tcc cgc atg aag 931 Leu Ser y Pro Arg Val lie Asp Ala Asp Thr Lys Ser Arg IVet Lys 265 270 275 gac atc ctg acc gat atc cag gac ggc acc ttc acc aag cgc ctc atc 979 Asp Ile Leu Thr Asp Ile G n Asp y Thr Phe Thr Lys Arg Leu Ile Page 323 seql i st cor r ect ed. t xt 280 285 290 gca aac gtt gag aac ggc aac acc gag ctt gag ggc ctt cgt gct tcc Ala Asn Val G u Asn G y Asn Thr G u Leu G u y Leu Arg Ala Ser 295 300 305 tac aac aac cac cca atc gag gag acc ggc gct aag ctc cgc gac ctc Tyr Asn Asn His Pro IIe G u G u Thr G y Ala Lys Leu Arg Asp Leu 310 315 320 325 atg agc tgg gtc aag gtt gac gct cgc gca gaa acc gct taagtttcac IVet Ser Trp Val Lys Val Asp Ala Arg Ala G u Thr Ala 330 335 ccctttgacg gct <210> 262 <211> 338 <212> PRT <213> Corynebacter i um gl ut ai cum 1027 1075 1124 1137 <400> 262 IVet Ala l e G u Leu Leu Tyr 1 G n Hi s Ar g Val Leu Pr o SIle Val Gy 145 Thr Ar g Leu Lys Al a 225 Ar g G n Gy Thr Pr o Leu Phe 115 Pr o Gy G n Gy Gy 195 Gy Phe 5 Val Leu Lys Al a Thr Al a Leu Gy Pr o Leu 165 I le G n G u Val Al a Ar g Ser G u 70 Ser Sy SIle Pr o Cys 150 Thr Pr o Al a Val Leu 230 l Ie Asp Al a Al a G n Asp Lys Sy 135 Leu Leu Thr Val Leu 215 Hi s Asp Ala Asp Ala Asp 10 Val y Tyr y Ser Ser y Val G u Val 40 G u Lys Ala Lys G u Ala Ala Trp Ala Asp Ala G u lie Phe Thr 90 Ala Leu Leu Phe G y 105 Pro Ala Asp Asp Ile 120 His Leu Val Arg Arg 140 lie Ala Val Asp n 155 Ser Tyr Ala Ala Al a 170 Thr Phe G u Ala G u 185 Leu Cys G y G y Thr 200 Thr G u Al a G y Tyr 220 G u Leu Lys Leu Ile 235 Leu G n Val Al a Val Asn Hi s SIle 125 G n Asp l Ie Thr G u 205 G u Val Leu Hi s Sy Phe Met SIe Leu Sy Val Thr Sy 175 Thr Leu G u Leu Page 324 Ph e G31u Ly s Thr G31y 305 Ly s Thr G31y G31y Ar g 275 Ar g Ar g Ar g I Ie 245 Tyr Ly s I Ie Ser Le u 325 Ser Le u As p A a Tyr 310 vb t As n Ser I Ie As n 295 As n Ser seql i st car r ect ed. t xt l'b t Asn Tyr Ser Val Ser 250 G31y Pr o Ar g Val I Ie As p 265 Leu Thr Asp I I e 03 n As p 280 285 Val G31u As n G31y As n Thr 300 As n Hi s Pr o I Ie (31u (31u 315 Tr p Val Lys Val As p Ala 330 As p A a 270 G31y G31u Th r Ar g Th r 255 As p Th r Le u G31y A a 335 <210O> 263 <21 1> 487 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ari cumn <220> <221 CDS <222> (487) <223> RXN03088 <400> 263 t cat ggcacc gggacct ggc at cgt agct a aggt t tctt t ccggcggcga tgaactcctc gaattggagg caaagaacta t gat gat t tc t ccgacgt ca at c I Ie ggt G31y ct g Le u gcc A a gag G31u Cgg Ar g aaa Ly s t cc Ser cgc Ar g gt c ValI t cc Ser gt t ValI gt g ValI t cc Ser g ac As p ac c Thr tt c Ph e gca A a gt c ValI gt c ValI t ac Tyr gca A a t ct Ser gga G31y ct c Le u gag G31u 75 gt a ValI ct t Le u cgc Ar g g ac As p tt t Ph e gga G31y t cg Ser aag Ly s gcg A a cgc Ar g gca A a ggC G31y g aa G31u cct Pr o cct Pr o gca A a 110 aag Ly s 15 cgc Ar g c ag (31n gct A a aag Ly s cgc Ar g 95 ccc Pr o at c I Ie g aa G31u cgc Ar g gcc A a gcc A a 80 gt a ValI g at As p at g vb t 1 g aa G31u ggt G31y gt c ValI t cc Ser gt a ValI ac c Thr aag Ly s ggt caa acc 03 y 03 n Th r gt a ValI ct c Le u ct c Le u ag g Ar g gag G31u gt c ValI tt t Ph e cca Pr o tt c Ph e g ac As p ggt G31y gt a ValI gca A a tt c Ph e 115 cca Pr a t cc Ser at t I Ie gcc A a at t I Ie gag G31u 1 00 ac g Thr cgc Ar g gca A a t ca Ser tt t Ph e g at As p cga Ar g at g vb t at g vb t gtg ctc gcc gac ccg ccc tat gag ctt gcg acg acg Page 325 seqI i st car r ect ed. t xt Val Leu Ala Asp Pro Pro Tyr G31u Leu Ala Thr Thr 120 125 <210O> 264 <21 1> 129 <212> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 264 'b t G31y O31n Th r Arg Ilie lieSer Gy 1 5 G31u ValI Pro Pro Ala G31y Th r Arg Pro 25 G31y Leu Phe Ser Ser Leu O31n Val Ar g 40 Val Leu As p I Ie Phe Ala G31y Ser G31y 55 Ser Ar g G31y Ala As p G31u ValI Val Leu 70 Val G u Val I Ie Ar g Ar g Asn Val As p Thr Val Ala 31u I'b t Lys Ala Ser Thr 100 105 Lys Phe Phe Thr Nib t Val Leu A a As p 115 120 Th r As p 10 Thr Ph e A a ValI ValI 90 Tyr Pr a A a Ser G31y Le u G31u 75 ValI Le u Pr a Ar g As p Ph e G31y Ser Ly s A a Tyr G31y Ar g G31u Le u As n Hi s Ser G31u 125 Ar g A a G31y G31u Pr a Pr a A a 110 Le u Ly s Ar g O31n A a Ly s Ar g Pr a A a I Ie O31u Ar g A a A a ValI As p Thr <210O> 265 <21 1> 639 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> 101). (616) <223> RXN02952 <400> 265 t ccaaaccgc t gt t tctgct cgt ggcgaat cct ggat at t cct gat ct tt gat ct cgct a cct cat tcac aagcgccggc ct t Le u gt t ValI tt t Ph e gcg gct A a cca Pr a gt c ValI t ct aaa Ly s tt t Ph e g ac As p gga cgc Ar g gcg A a aag Ly s gca gct A a t ac Tyr at t I Ie c ac gaa t cc 03 u Ser 15 t cc ggc Ser 03 y gt g ggt Val G31y t ca tt g Page 32E gaagt aat t g aaat ccgaga at g agc t cc cca gca I'b t Ser Ser Pr o Ala 1 gat ggc ct g at c gct Asp 03 y Leu Il e A a at c t tc aag at g tt c Ilie Phe Lys I'b t Phe ct g ccc acc at c at t Leu Pr o Thr Ilie Ilie gtt ctc gac cac gcc Al a at c SIle gt g Val gaa G u cag G n gac Asp aac Asn 150 ct a Leu Ser cca Pr o gca Al a cgc Ar g t ac Tyr 120 gac Asp agc Ser tcg Ser Gy ttt Phe acg Thr aac Asn agt Ser t cc Ser ttt Phe at c SIle 170 Thr acc Thr 75 gaa G u cgc Ar g gt g Val tt c Phe gcc Al a 155 t cc Ser Al a t ac Tyr gat Asp gca Al a caa G n cgt Ar g 140 gat Asp gtt Val seql i st cor r ect ed. t xt Arg His Ser Leu Val Leu Asp ttg cga gca gtt gtc gta ccc Leu Arg Ala Val Val Val Pr o ttc ggc act gaa gct ggc gca Phe G y Thr G u Al a G y Al a 95 gct ggc gaa tta gcg aca ctc Ala Gy G u Leu Ala Thr Leu 110 115 ggc ctt ggg ggc gca acc gcg G y Leu G y G y Al a Thr Al a 125 130 cgc acc act ggc gtg acc ccg Arg Thr Thr y Val Thr Pro 145 ctt tct caa agg aca cga cgg Leu Ser G n Arg Thr Arg Arg 160 taaggcattg aagcatttgg agg Hi s acc Thr gac Asp 100 at g Met aac Asn gga Gy aaa Lys <210> 266 <211> 172 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 266 IVet Ser Ser Pro Ala Leu Asp Ala Ala 1 5 Asp Gy Leu lie Ala Val Thr Pro Val 25 lie Phe Lys IVet Phe Phe Asp Val Leu 40 Leu Pro Thr Ile Ile Ala Ala Ser Ala 55 Val Leu Asp His Ala l e Arg Pro Leu 70 Val Val Pro Thr y Val Phe Ala Al a Ala y Ala Asp lie u Arg Arg Val 100 105 Ala Thr Leu IVet Leu G n Asp Tyr Ser 115 120 Ala Thr Ala Asn G n Asp Ala Asp Leu 130 135 Val Thr Pro Gy G u Asn Phe Ser Ser 145 150 Lys G n Arg 10 Phe Thr Al a Asp Pro Lys G y Thr Al a Phe Thr Tyr Thr G u Asp 90 Asn Arg Ala Ser Val G n Ser Phe Ar g 140 Phe Al a Asp 155 Page 327 Leu Ser Thr Ar g Leu Phe Al a Gy 125 Ar g Leu Al a Tyr I le Hi s Ar g Gy Gy 110 Leu Thr Ser G u Ser Val Ser Al a Thr G u Gy Thr G n seqi i st car r ect ed. t xt Thr Ar g Ar g Lys Ar g Leu Asn Ser Ar g I Ie Ser Val 165 170 <210O> 267 <21 1> 1044 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (1021) <223> RXN00513 <400> 267 cacagcgt ac gcacgagct t gaggat ct tt cagaact caa tt t tggccaa ggct cct gt g at tccggaag gact gt tct g cat t gaat t g gat gccgat a at g gcg ggt [vb t A a 03 y t tg tt t Leu Phe t cc Ser ct g Le u gcg A a ggc G31y ac a Thr g ac As p t cc Ser t gc CYS gga G31y gct A a 1 50 g ac As p gct A a gct A a gcg A a cgc Ar g gt c ValI ac c Thr ccc Pr a gcg A a ct g Le u 120 g aa O31u aac As n gcg A a gcg A a gca A a g aa O31u at c I Ie gcg A a gt g ValI ct g Le u ac c Thr g at As p gt g ValI cgt Ar g gt g ValI 1 70 gcc A a cca Pr a at t I Ie cag O31n gct A a gcg A a 75 gt c ValI gt c ValI caa O31n G31y cgc Ar g 155 gaa O31u ggc (31y ac g Thr gcc A a g aa O31u g ac As p c ac Hi s g ac As p t cc Ser gt c ValI ac g Thr 140 g at As p ggc G31y gcc A a gag O31u cgc Ar g gcc A a 45 ct t Le u cgc Ar g gcc A a g aa O31u aac As n 125 g ac As p gag O31u at c I Ie g ac As p cgt Ar g at g vb t gga G31y gca A a t cc Ser g ac As p ct c Le u 110 ccc Pr a at c I Ie c aa O31n g ac As p at g 1 90 cga Ar g 15 cct Pr a tt t Ph e tt g Le u cgg Ar g ac c Thr 95 g aa (31u aaa Ly s at g vb t tt c Ph e t cc Ser 1 75 at c I Ie aaa Ly s ggt G31y g aa (31u ccg Pr a c ag O31n ggc O31y g at As p cgc Ar g gt t ValI gt c ValI 1 gcg A a tt c Ph e 1 gca A a gca A a ggc O31y g at As p at c I Ie tt c Ph e gca A a t gt cys cgt Ar g 145 at c I Ie at c I Ie ac c Thr tt a Le u tt c Ph e gt g ValI at c I Ie gca A a ggc O31y ggt O31y O31y 130 cgc Ar g t gc cys gag (31u cgc Ar g t cc Ser t ac Tyr O31y cgc Ar g g aa O31u gt c ValI 115 c ac Hi s at c I Ie gct A a cgc Ar g gcg A a ccg Pr a gt c ValI tt g Le u gt g ValI ccc Pr a 1 00 gcg A a ct g Le u gcc A a cgc Ar g gcc A a 1 gaa gcg ct g t ac (3 u A a Leu Tyr 1 Page 328 seqI i st car r ect ed. t xt agc cct gca gat t tt gaa aaa t tc cgc gcg gcc gt c gac Ser Pr o Al a Asp Phe O31u Lys Phe Ar g Al a Al a Val As p 200 205 210 ct g gcc aac at g acg gaa t tt ggc aaa acc gaa ct t ct g Leu Al a As n I'b t Thr G3 u Phe G3 y Lys Thr G3 u Leu Leu 215 220 225 ct t ct g gaa gac at c gga t ac aac gca gt g at c t ac cca Leu Leu O31u As p IIe G31y Ty r Asn Al a Val I Ie Ty r Pr o 230 235 240 ct g cgc at t gcg at g gga cag gt c gaa caa gct ct c ggc Leu Arg Ile Al a 'bt G31y 1n Val O31u 1n Al aLeu (1y 250 255 aac acc gga atc caa acc gac tgg gtc gac cgg atg caa Asn Thr G31y Ilie O31n Thr Asp Tr p Val Asp Ar g /b t O31n 265 270 agg ct g t at gag ct g ct g cgc t ac aac gag t ac aac gct Ar g Leu Tyr O31u Leu Leu Ar g Tyr Asn O31u Tyr Asn Al a 280 285 290 caa gt a t tc acc t at t cc gct gac agc t ac aag ccc at c O31n Val Phe Thr Tyr Ser Al a Asp Ser Tyr Lys Pr o Ilie 295 300 305 t aacccgcct at at at aagg agt <210> 268 <211> 307 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn ccg Pr o gcg Al a ac c Thr at t I Ie 260 cga Ar g g ac As p ct g Le u c ag O31n ct g Le u 245 gca Al a t cc Ser c ag O31n 1021 1 044 <400> 268 [vb t Al a 03y Leu Phe Ser Ser Ala Val Ala Pro Thr Ou Al1 Al a G31y As p li1e Ph e Al a cys Ar g 145 Ar g Ser Tyr G31y Ar g (31u ValI 115 Hi s I Ie Al a Le u Ser Thr Thr vb t G31y As p Al a Al a Al a Al a Le u Le u Al a Hi s Ly s 135 ValI Al a Ar g 40 ValI Thr Pr o Al a Le u 120 (31u As n Pr o 25 Al a ValI (31u ValI Ar g 1 05 (31u ValI (31u 10 O3 u I I e Al a I Ie O31n O31u Al a Al a As p Val Al a Hi s Leu Val Asp 90 Thr Val Ser Asp 03 n Val Val O31y Thr 140 Ar g Ar g Asp 1 Page 329 Ar g Al a Le u Ar g Al a O31u As n 125 As p (31u Ar g vb t O31y Al a Ser As p Le u 110 Pr o I Ie O31n Ar g Pr a Ph e Le u Ar g Th r O31u vb t Ph e seql i st cor r ec Ile Cys Ala Arg Thr Asp Ala Ala Gy Val Gu 165 170 lie G u Arg Al a Lys Al a Tyr Al a Asp Al a G y 180 185 Thr G u Ala Leu Tyr Ser Pro Ala Asp Phe G u 195 200 Val Asp Ile Pro Leu Leu Ala Asn IVet Thr G u 210 215 Leu Leu Pro Ala G n Leu Leu G u Asp Ile y 225 230 235 Tyr Pro Val Thr Leu Leu Arg Ile Al a Iet G y 245 250 Leu G y Asp ile Al a Asn Thr G y ile G n Thr 260 265 IVet G n His Arg Ser Arg Leu Tyr G u Leu Leu 275 280 Asn Ala Phe Asp n G n Val Phe Thr Tyr Ser 290 295 Pro Ile Phe 305 <210> 269 <211> 957 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(934) <223> RXN01152 <400> 269 agtgaaggat ctgatgtggt ttgaacaagc cctggaagcc agttcaatca agacaagcac acagaagaaa gtgagggctc ted. txt G y lle Al a Asp Lys Phe 205 Phe G y 220 Tyr Asn G n Val Asp Trp Arg Tyr 285 Al a Asp 300 Ser 175 I e Al a Thr Val G n 255 Asp G u Tyr ccg Pr o cat Hi s gga Sy gt c Val gtt Val caa G n acc Thr at c SIle t cc Ser cac Hi s ggc Sy at t SIle cac Hi s at g Met ct g Leu cgc Ar g gaa G u gac Asp tct Ser at g Met gtt Val cca Pr o at c SIle 45 gat Asp cct Pr o cag G n gga G y at c SIle t ac Tyr cgt Ar g ctc acc Leu Thr 15 acc acc Thr Thr ggc gcc G y Al a ttg tgc Leu Cys ggc gct G y Al a 80 Page 33C tatctggtaa attaacgccg atg ccc tac tca ggt IVet Pro Tyr Ser y 1 gac gct aaa cgc cgc Asp Ala Lys Arg Arg tac cac acc cac cgt Tyr His Thr His Arg gat gag ggc act gtt Asp G u G y Thr Val ttc cgt cac ctc atg Phe Arg His Leu IVet gca gtt att tat cca Ala Val lie Tyr Pro IND seqI i st car r ect ed. t xt Saag gac tct gca cag at t ct ggt cgag ggc gat at t t t ccct ggc gcc 403 SLys Asp Ser Al a (1nIIe Leu Val (1u 1y AspIle PhePr o 1y Al a 95 100 fl cga gtt ct g gaa gct ggc gct ggt t cc ggt gca ct g t cc at g gcg ct g 451 c.Ar g Val Leu G31u Ala G31y Ala G31y Ser G31y Al a Leu Ser N/t Al a Leu 105 110 115 ci ct t cgt gca gt g ggt gaa aag ggc aat gt c at c t cc t ac gaa at c cgt 499 Leu Ar g Al a Val O31y O31u Lys O31y Asn Val Ilie Ser Tyr O31u Ilie Ar g 120 125 130 cI gag gat cac ct g gag t ac gca gt c t cc aac gt g gag gag t ac t tc ggt 547 (3 OuAsp Hi sLeu (1u Tyr Al aVal Ser Asn Val (1u (1u Tyr Phe (1y 00 135 140 145 Sgag cgt cca gca acc t gg gat cca cgt ct t ggt gac ct g aaa gaa gt c 595 31 Ou Ar g Pr o Al a Thr Tr p Asp Pr o Ar g Leu G31y Asp Leu Lys O31u Val .C 150 155 160 165 Sacc gt t gag gat ct cggc gga cct gt t gac cgc at catc t tg gat at g 643 c-1 Thr Val O31u Asp Leu G31y G31y Pr o Val Asp Arg Ilie Ilie Leu Asp I'b t 170 175 180 ct t gag ccg t gg gaa at g ct g gag acc t gc aag gat ct t ct c at c cct 691 Leu G u Pr o Tr p G u [vb t Leu G u Thr Cys Lys Asp Leu Leu Ilie Pr o 185 190 195 ggt ggt gtg t tt at gacg tat gt ggcg acc gt gcca cag ct gatg aag 739 G31y G31y Val Phe I'b t Thr Tyr Val Al a Thr Val Pr o O31n Leu I'b t Lys 200 205 210 gtc atg gaa ggc atc cgc gag caa aaa tgc ttc acg gag cca cgc gcg 787 Val N/b t 31u G31y Ilie Ar g 31u O31n Lys Cys Phe Thr O31u Pr o Ar g Al a 215 220 225 t gg gaa tct t tg gt t cgt gat t gg aag gt ggag ggc t tggca aca cgc 835 Trp O31u Ser Leu Val Ar g Asp Tr p Lys Val O31u G31y Leu Al a Thr Ar g 230 235 240 245 cct gag cac cgc at g aat gcc cac acc gcg t tc t tg gt g t tg acc agg 883 Pro O31u Hi s Ar g I'b t Asn Al a Hi s Thr Al a Phe Leu Val Leu Thr Ar g 250 255 260 cgt t t g gct gat ggc gt g gag cct cct cgt ccg cag cgt aag gca cgt 931 Ar g Leu Al a Asp O31y Val O31u Pr o Pr o Ar g Pr o O31n Ar g Lys Al a Ar g 265 270 275 cga taaaaagacc tagttggagg gcg 957 Ar g <210> 270 <211> 278 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 270 I'bt Pro Tyr Ser Gy Pro Phe On Ala G1y Asp Arg Val On Leu Thr 1 5 10 Asp Ala Lys Arg Arg His Phe Thr Ilie Ilie Leu O31u Pro O31y Thr Thr 25 Tyr Hi s Th r Hi s Ar g 31y O31n I Ie Al a Hi s Asp Asp Ilie Ilie Oy Ala 40 Page 331 seqi i st car r ect ed. t xt Asp (31u G31y Thr Val Val Hi s Ser Thr l'bt G31y Ser Asp 55 Tyr Leu Cys Ph e Al a I Ie Le u Ser (31u 145 As p I Ie As p Pr o Thr 225 G31y Le u O31n Hi s I Ie Pr o vb t 115 G31u Tyr Lys5 Le u Le u 1 95 Le u Pr o Al a Le u Lys5 275 vb t Pr o Al a Le u Ar g G31y ValI 1 65 vb t Pr a Lys5 Al a Ar g 245 Ar g Ar g As p As p ValI Ar g As p 135 Ar g ValI G31u G31y vb t 215 G31u G31u Le u ValI Al a G31u 1 ValI Le u Al a As p Tr p 1 Ph e G31y Le u Ar g As p 265 Ser li1e G31y G31u Tyr Tr p 1 G31y vb t Thr Ar g Ar g 235 As n ValI vb t Le u Al a Lys5 Al a 140 As p G31y Le u Tyr G31u 220 As p Al a (31u Pr a ValI G31y G31y 125 ValI Pr a Pr a O31u ValI 205 O31n Tr p Hi s Pr a G31y G31y G31y ValI As n Le u As p 1 Cys Thr Cys ValI Al a 255 Ar g <210O> 271 <21 1> 2025 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 CDS <222> (2002) <223> RXN00787 <400> 271 ccagcccgcc caat aaat aa t t tct ct ctt ct aat tgcgg agcct cat at at tgagt acg gt att t tgaa acacct tcag ccccctt t tt aggagccaca gt g t ct cag cct ct c Val Ser O31n Pro Leu 1 agc aag cgt ctc agc ata cga aaa gca ctc gcc agc gcc t tc at a gt t Ser Lys Arg Leu Ser lieArg Lys Ala Leu Ala Ser Al a Phe I Ie Val 15 gcg ct ggcg t tt t cg ctt t cc cca gt agcc aaa gcc caa gcc aat gaa Page 332 seql i st cor r ect ed. t xt Ala Leu Ala Phe Ser Leu Ser Pro Val Ala Lys Ala G n 30 Al a Asn G u act Thr gat Asp cag G n t ac Tyr caa G n tta Leu ggc G y at t SIle 150 tgc Cys aac Asn tgc Cys cag G n tct Ser 230 gca Al a cca Pr o at c SIle acg Thr ggc G y at t SIle gga G y gt c Val gac Asp 120 gca Al a ttg Leu gt a Val at c SIle gct Al a 200 ctt Leu acc Thr gt a Val gaa G u gtt Val 280 at c SIle cag G n gac Asp acc Thr at c SIle at c SIle acc Thr t cc Ser caa G n 170 ct a Leu gt c Val gat Asp gat Asp at t SIle 250 cgc Ar g gcc Al a gt g Val acc Thr at c SIle 75 ggc G y ctt Leu aac Asn gat Asp gat Asp 155 gaa G u aat Asn ggt G y gcg Al a gt g Val 235 gat Asp ttt Phe aac Asn ttg Leu cgt Ar g t cc Ser gaa G u ggc G y agc Ser acc Thr 140 ggc G y ct g Leu gtt Val ggt G y ct g Leu 220 aca Thr gag G u tgg Tr p aca Thr gac Asp 45 at c SIle gac Asp aca Thr ggc G y ct g Leu 125 gcc Al a at c SIle gct Al a gat Asp ggc G y 205 aca Thr aaa Lys gat Asp aaa Lys gtt Val 285 aat Asn gat Asp cgc Ar g gaa G u ccc Pr o 110 cag G n gcc Al a gcc Al a caa G n cca Pr o 190 act Thr cga Ar g at t SIle aca Thr at c SIle 270 acc Thr t ca Ser gca Al a acc Thr gca Al a 95 t cc Ser cct Pr o gag G u aac Asn agt Ser 175 gca Al a t ac Tyr gcc Al a gat Asp gaa G u 255 cct Pr o gac Asp ggc G y gca Al a gac Asp gac Asp cga Ar g cga Ar g ct c Leu tgc Cys 160 gga G y gcg Al a gcg Al a gcc Al a ggg G y 240 ct a Leu gt a Val cca Pr o t cc Ser aaa Lys gt a Val gtt Val gga G y ggc G y ccc Pr o 145 acc Thr at c SIle cgc Ar g gat Asp agt Ser 225 gca Al a ttc Phe gag G u aca Thr at g let caa G n ggt G y gag G u aat Asn ttc Phe 130 gaa cca Pr o aac Asn gaa G u gct Al a 210 agg Ar g tcg Ser ct c Leu cca Pr o gt a Val 290 aca Thr gcc Al a ct g Leu at g Met gca Al a 115 acc Thr ggc G y ccg Pr o ct g Leu gaa G u 195 t cc Ser caa G n gaa G u acc Thr ggt G y 275 ct c Leu gct Al a t cc Ser acc Thr gga G y 100 gac Asp ccc Pr o gga G y gat Asp gtt Val 180 ctg Leu gac Asp t ac Tyr caa G n gac Asp 260 gaa G u acc Thr ggg caa ggc gga atc aag ctt gaa gcc caa ctc cat act gaa gag gct Page 333 1027 seql i st cor r ect ed. t xt G y n G y G y lie Lys Leu G u Ala G n Leu His Thr Su G u Al a 295 300 cca Pr o 310 ttc Phe t ca Ser gaa G u gaa G u aat Asn 390 t ca Ser gat Asp gaa G u gca Al a acg Thr 470 ct c Leu ttt Phe tct Ser ct c Leu act Thr 550 t ac Tyr ccc Pr o gaa G u tct Ser 360 acc Thr aca Thr gca Al a gat Asp cac Hi s 440 gt a Val ctt Leu aat Asn ttc Phe ggc G y 520 gt g Val cag G n ct g Leu ctt Leu 330 ggc G y agc Ser gag G u gaa G u cct Pr o 410 gca Al a t at Tyr aca Thr at c SIle gat Asp 490 acc Thr tcg Ser t ac Tyr ccc Pr o cgt Ar g 315 ggt G y acc Thr gga G y cgc Ar g cat Hi s 395 gt c Val ggt G y gcg Al a act Thr caa G n 475 acg Thr cca Pr o ct a Leu ct a Leu aca Thr 555 ggt G y gt a Val aac Asn gat Asp ttc Phe 380 agc Ser aca Thr gag G u ct g Leu ttt Phe 460 gcg Al a t ca Ser gt g Val agg Ar g cca Pr o 540 ttg Leu cgg Ar g cgc Ar g aac Asn t ac Tyr 365 gga G y t cc Ser cct Pr o aaa Lys cct Pr o 445 gac Asp ttc Phe t at Tyr agt Ser agc Ser 525 agt Ser gaa G u tgc Cys gga G y tgt Cys 350 ct c Leu aaa Lys gt c Val ggc G y gt c Val 430 gt c Val caa G n agc Ser gcg Al a gca Al a 510 cca Pr o ggt G y t at Tyr act Thr at c SIle 335 gac Asp aac Asn gt a Val gat Asp cag G n 415 t cc Ser gac Asp at c SIle cca Pr o gac Asp 495 gca Al a tgg Tr p caa G n gaa G u cgg Ar g 320 caa G n acc Thr ggg G y gat Asp ctt Leu 400 tgg Tr p t cc Ser t ac Tyr gac Asp aac Asn 480 gac Asp aat Asn caa G n gat Asp ct c Leu 560 305 gt c Val aac Asn gat Asp cag G n gaa G u 385 acc Thr ttc Phe at c SIle ggc G y agt Ser 465 cgg Ar g aac Asn ttg Leu ggt G y gaa G u 545 gt g Val t ca Ser gcg Al a gcc Al a gac Asp 370 t ca Ser gag G u aca Thr at c SIle caa G n 450 t cc Ser gca Al a ggg G y ttc Phe ggc G y 530 gat Asp gca Al a ttt Phe t cc Ser at c l Ie 355 at t SIle aca Thr gct Al a tcg Ser gtt Val 435 gaa G u gcg Al a gag G u ct c Leu gag G u 515 acc Thr gt a Val gaa G u gat Asp gtt Val 340 t ac Tyr cca Pr o at c SIle gca Al a gcc Al a 420 cca Pr o ct g Leu ctt Leu at a SIle aaa Lys 500 aaa Lys caa G n t cc Ser gcg Al a aat Asn 325 gca Al a ct c Leu acg Thr gga G y gca Al a 405 gct Al a gga G y cgc Ar g ggc G y gag G u 485 act Thr agt Ser t ac Tyr gca Al a ttt Phe 565 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 gga gac cct gtt gac cca ccg gtt ttc gct tca ttg acg gga gca acc Page 334 G31y As p Pr o cca agc acc Pr o Ser Thr t cc gag gca Ser G3 u Al a 600 ggg ct g ggt G3 y Leu G3 y 615 ct g aga aga Leu Arg Arg 630 <210> 272 <211> 634 <21 2> PmT As p 570 ac c Thr g aa O31u at t I Ie aat As n seqi i st car r ect ed. t xt Pr o Pr o Val Phe Al a Ser Leu Thr G31y Al a Th r 575 580 ccc cca t ca gat gt t gcg gaa gat gaa caa at c Pro Pro Ser As p Val Ala O31u As p O31u O31n IIe 590 595 gaa gac t ca agc agt t tc ccc at c gt g t gg at t O31u As p Ser Ser Ser Phe Pr o I Ie Val Tr p I Ie 605 610 ggc t ta ggc at a at c at t ggt t tg at c t tt gcg G3 y Leu G3 y I I e I I e I I e G3 y Leu I I e Phe Al a 620 625 t aagccct aa aagat aaaga gt c 1891 1 939 1 987 2025 <21 3> Cor ynebact er i urn gi ut ani cumn <400> 272 Val Ser On Pro Ser Al a Phe I I e Al a 03 n Al a As n Ser l'b t Thr Al a Lys 03 n Al a Ser Val 03 y Leu Thr Val O31u [ib t 31y 1 00 O3 y As n Al a As p 115 O31y Phe Thr Pr o 130 Pro O31u 31y O31y 145 Thr Pr o Pr o As p I I e As n Leu Val 1 80 Ar g O31u O31u Leu 1 95 As p Al a Ser As p 210 Le u ValI O31u O31n Thr Tyr cys Thr I Ie As n ValI 1 65 li1e O31u Al a Ser Al a Thr As p O31n 70 Tyr O31n Le u O31y I Ie 150 Cy s As n Cy s O31n Ly s Le u Pr o Al a Le u O31y As p I Ie Ly s 135 ValI O31u Thr I Ie Ser 215 Ar g Al a Thr 40 O31y I Ie O31y ValI As p 120 Al a Le u ValI I Ie Al a 200 Le u Le u Ph e 25 vb t O31y As n As n Thr 1 05 Thr Le u ValI Al a O31y 1 85 O31y Thr I Ie Le u ValI Thr I Ie 75 O31y Le u As n As p As p 1 55 O31u As n O31y Ly s Pr o As p li1e As p Thr O31y Le u 125 Al a I Ie Al a As p O31y 205 Al a ValI As n As p Ar g O31u Pr o 110 O31n Al a Al a O31n Pr o 1 Thr Le u Al a Ser Al a Thr Al a Ser Pr o O31u As n Ser 1 Al a Tyr Al a Ly s O31y Al a As p As p Ar g Ar g Le u cys 1 O31y Al a Al a Asp Ala Leu Thr Arg Ala Ala 220 Page 335 Ser Arg 225 G n Tyr Asn Ser Tyr Th 230 seql i st cor r ect ed. t xt r Ser Asp Val Thr Lys I le Asp G y 240 Al a Phe G u Thr Hi s 305 Val Asn Asp G n G u 385 Thr Phe SIle Gy Ser 465 Ar g Asn Leu Gy G u 545 Val Leu G u Thr Gy 275 Leu G u Phe Ser SIle 355 I le Thr Al a Ser Val 435 G u Al a G u Leu G u 515 Thr Val G u Gy Ser 245 Leu Thr let Al a Asn 325 Al a Leu Thr Gy Al a 405 Al a Gy Ar g Gy G u 485 Thr Ser Tyr Al a Phe 565 Thr Al a Pr o SIle Gy Pr o 310 Phe Ser G u G u Asn 390 Ser Asp G u Al a Thr 470 Leu Phe Ser Leu Thr 550 Gy Pr o Al a G n Ser G n 295 G n Lys Lys SIle SIle 375 Val G u Leu Thr Al a 455 Hi s Thr Gy G n Al a 535 Asp Asp Ser G u lie 250 Ser Arg 265 Ser Al a G y lle G y Leu G y Leu 330 Val G y 345 Arg Ser Ile G u G u G u His Pr o 410 Pro Al a 425 Phe Tyr G u Thr Tyr Ile Arg Asp 490 Phe Thr 505 Ile Ser Thr Tyr Leu Pro Val Asp 570 Ser Thr 585 235 Asp Phe Asn Lys Ar g 315 Sy Thr Sy Ar g Hi s 395 Val Sy Al a Thr G n 475 Thr Pr o Leu Leu Thr 555 Pr o Asp Lys Val 285 G u Ar g Ar g Asn Tyr 365 Sy Ser Pr o Lys Pr o 445 Asp Phe Tyr Ser Ser 525 Ser G u Val Thr SIle 270 Thr Al a Cys Sy Cys 350 Leu Lys Val Sy Val 430 Val G n Ser Al a Al a 510 Pr o Sy Tyr Phe G u 255 Pr o Asp G n Thr SIe 335 Asp Asn Val Asp G n 415 Ser Asp SIe Pr o Asp 495 Al a Tr p G n G u Al a 575 Pro Pro Ser Asp Val Al a 590 Page 336 seqI i st car r ect ed. t xt O31u As p 31u O31n I Ie Se r O31u Al a Thr O31u O31u Asp Ser Ser Ser Phe 595 600 605 Pro I Ie Val Tr p I Ie (1y Leu (1y Val Ile(3y Leu (1y I Ie I Ie I Ie 610 615 620 G31y Leu Ilie Phe Ala Leu Ar g Ar g Lys Asn 625 630 <210> 273 <211> 576 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CIDS <222> (553) <223> RXN01302 <400> 273 tggggct gcg t ggt gt t ct t cat cat cgcc accgct t t ga cct ggat ct a ct acgcccgc ccgaacgctc cattcccggg ataaaccgaa aggccaatcc act aca act Thr Thr Thr t ct t ct Ser Ser agt tt c Ser Phe ct t cag Leu 03 n t tc gat Phe As p t gc t cg Cys Ser cag gca 03 n Al a cca cgt Pr o Ar g cca acc Pr o Thr t cc gcg Ser Al a 135 aca tt g Thr Leu 1 ggg G31y ct a Le u ggc G31y aaa Ly s t gg Tr p gt g ValI ggc G31y cgc Ar g 120 aag Ly s aag t ct t ct Lys Ser Ser aga aaa ggc Ar g Lys G31y gga cgc caa G31y Ar g O31n gt c gt g cgc Val Val Ar g aaa gt g t at Lys Val Tyr 75 gat tac cca Asp Tyr Pr o t gc cct cgt Cys Pr o Ar g 1 05 at c cgc t ac Ilie Ar g Tyr cca agg aac Pr o Ar g Asn g aa O31u ac c Thr gcc Al a t cc Ser gt a ValI act Thr ggt G31y cca Pr o gcc Al a 140 aag Ly s gt c ValI g at As p 45 ac a Thr aaa Ly s ac c Thr gca Al a t ac Tyr 125 t gg Tr p at c I Ie ggt G31y 30 gt g ValI cat Hi s g ac As p ggt G31y t ca Ser 110 at c I Ie gcg Al a ccc Pr o g aa O31u t at Tyr gt g ValI gt g ValI g at As p t cc Ser ggc G31y cgg Ar g tt c Ph e c ag O31n aac As n t gc Cys ac c Thr ccc Pr o t ac Tyr ct a Le u 130 t gg Tr p aag Ly s c ag O31n cga Ar g ac g Thr t gg Tr p g ac As p ac c Thr 115 gt t ValI cgt Ar g ct c Le u at t I Ie t gg Tr p ggc O31y g aa O31u aat As n 1 00 t ac Ty r g at As p ggc (31y tagaaacccc agaaaagcgc aaa Page 337 seqI i st car r ect ed. t xt <210O> 274 <21 1> 151 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 274 I'bt Thr Thr Thr Thr Ser Ser Oy Lys Ser Ser Ou Lys lieAsn Pro Le u G31y Ar g As n li1e vb t Tr p ValI CYS 145 Lys O31n Ar g Thr Tr p As p Thr 115 ValI Ar g G31y Ph e A a Ser Ser G u Ser vb t A a Ph e O31n As p Ser A a Ar g Thr A a 135 Le u Ar g G31y ValI Ly s As p cys 1 li1e Pr o G31y O31n Ar g Tyr Pr o Ar g Tyr As n Thr A a Ser ValI Thr G y Pr o A a 140 ValI As p Thr Ly s Thr A a Tyr 125 Tr p Ser Ph e G31y G31y A a Ph e Gy le <210O> 275 <21 1> 466 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani curn <220> <221 OIDS <222> (466) <223> FRXA01302 <400> 275 t ggggct gcg t ggt gt tct t cat cat cgcc accgct t tga ccgaacgctc cattcccggg ataaaccgaa aggccaatcc t ct Ser agt Ser ct t Le u t tc ggg G31y ct a Le u ggc G31y aaa t ct Ser aaa Ly s cgc Ar g gt g t ct Ser ggc G31y caa O31n cgc g aa O31u ac c Thr gcc A a t cc at c I Ie ggt O31y gt g ValI cat aac ccc As n Pr o 15 tct gaa Ser 03 u t tt t at Phe Tyr ggc gt g Page 33E cct ggat ct a ct acgcccgc at g act aca act act I'b t Thr Thr Thr Thr 1 ct c t tc aag ct c ggc Leu Phe Lys Leu 03 y ggc cag cag att ttc O3 y 03 n 03 n Il e Phe cgc aac cga t gg gct Ar g Asn Ar g Tr p Ala aac t gc acg ggc t cc INO seqI i st car r ect ed. t xt SPhe Asp Lys Val Val Ar gSer Thr Hi s 1y Val As nCy sTh r 1y Se r 60 t gc t cg t gg aaa gtg t at gt a aaa gac ggt gt g at c acc tgg gaa t cc 355 fl Cys Ser Tr p Lys Val Ty r Val Lys Asp G31y Val I Ie Thr Tr p O31u Ser 70 75 80 cag gca gt g gat t ac cca act acc ggt gcg gat at g ccc gac aat gaa 403 31n Ala Val As pTy rPr oThr Thr (1y AaAs pIb tPr oAs pAs n (1u 95 100 cca cgt ggc tgc cct cgt ggt gca tca t tt t cc tgg tac acc tac tcc 451 (i Pr o Arg G31y Cys Pr o Ar g G31y Ala Ser Phe Ser Tr p Ty r Thr Ty r Ser 105 110 115 00 Scca acc ggc atc cgc 466 SPr o Thr G31y Ilie Ar g 120 S<210> 276 C1 <211> 122 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 276 I'bt Thr Thr Thr Thr Ser Ser Gy Lys Ser Ser Ou Lys lieAsn Pro 1 5 10 Leu Phe Lys Leu G31y Ser Phe Leu Arg Lys G31y Th r Val G31y Se r (31u 25 31y O31n O31n I Ie Phe L eu O31n O31y O31y Ar g O31n Ala Asp Val Phe Tyr 40 Ar g Asn Arg Tr p Ala Phe Asp Lys Val Val Ar g Ser Thr Hi s O31y Val 55 Asn Cys Thr O31y Ser Cys Ser Tr p Lys Val Tyr Val Lys Asp O31y Val 70 75 Ilie Thr Tr p O31u Ser O31n Ala Val Asp Tyr Pr o Thr Thr O31y Ala Asp 90 I'b t Pr oAsp Asn O31u Pr oAr g G31y Cys Pr oAr g G31y Ala Ser Phe Ser 100 105 110 Tr p Tyr Thr Tyr Ser Pr o Thr G31y Ilie Ar g 115 120 <210> 277 <211> 2503 <212> DNA <213> Cor ynebact er i urn gi ut anri curn <220> <221> OIDS <222> (2503) <223> RXN01308 <400> 277 t t t cct ggt a cacct act cc ccaacccgca t ccgct accc at acat cggt ggcgt gct ag ttgatatgtc cgcgaagcca aggaacgcct gggcgatccg gtg ctg gcg tgg cgc 115 Val Leu Ala Trp Arg 1 Page 339 seql i st cor r ect ed. t xt gac att gta gaa acc cca gaa Asp Ile Val u Thr Pro u aag cgc aaa Lys Arg Lys ggc Gy gct Al a at t SIle gct Al a t ac Tyr gat Asp ct c Leu cac Hi s t cc Ser 150 at c SIle at c SIle t ac Tyr cac Hi s gca Al a 230 cgt Ar g act Thr ggt Gy gca Al a gga Gy act Thr tgg Tr p act Thr at g let 120 at g Met gat Asp cct Pr o aag Lys cgc Ar g 200 gat Asp gat Asp ctt Leu gcg Al a ct c Leu cat Hi s ace Thr ttc Phe gcc Al a gtt Val ggt Gy gaa G u gct Al a act Thr 170 ttc Phe t ac Tyr ace Thr t cc Ser ct g Leu 250 cgt Ar g at c SIle gt g Val gtt Val tt g Leu 75 gac Asp ccg Pr o t cc Ser gcc Al a gac Asp 155 gac Asp cat Hi s acg Thr tac Ty r cca Pr o 235 caa G n tgg Tr p cgc Ar g t ac Tyr at t SIle cag G n ct c Leu gaa Gu aac Asn cgc Ar g 140 t cc Ser ggc Gy gtt Val gac Asp acc Thr 220 gcg Al a aaa Lys ggt Gy gtt Val acc Thr 45 ccc Pr o at g Met cca Pr o tct Ser at t SIle 125 t ac Tyr acc Thr gca Al a gac Asp tct Ser 205 cca Pr o ctt Leu gat Asp gaa G u cag G n at c SIle gca Al a at c SIle cca Pr o ggc Gy 110 ccg Pr o aag Lys aaa Lys ct c Leu aag Lys 190 cct Pr o ggt Gy gcc Al a ggc Gy gaa G0u 270 t at Ty r cgc Ar g at g let ggc Gy gca Al a 95 gac Asp gt g Val ggc Gy ttt Phe gcc Al a 175 aag Lys ttc Phe aaa Lys gcc Al a t ca Ser 255 ggc Gy gca t at Al a Tyr gag gaa G u G u caa t ac G n Tyr tcg cag Ser G n gga gt g G y Val t ca cca Ser Pro tgg tac Trp Tyr acc cgc Thr Arg acc aag Thr Lys 145 gct gat Al a Asp 160 ttt gcc Phe Al a acg ccg Thr Pro ctc gt g Leu Val ttc ctc Phe Leu 225 act cca Thr Pro 240 gtt gta Val Val atg ggt lVet y gt a t cc cag Val Ser G n gcc Al a ggc Gy gt g Val gcg Al a caa G n aac Asn acg Thr 130 gtt Val gaa G u at g Met t ac Tyr gaa G u 210 act Thr aat Asn gat Asp aag Lys at g Mbt ccc Pr o tct Ser ct g Leu act Thr t cc Ser 115 cct Pr o gtt Val tgg Tr p ggc Gy ttc Phe 195 tta Leu gca Al a gcc Al a ccc Pr o tgg Tr p 275 gag G u gac Asp t ac Tyr t cc Ser ttc Phe 100 agc Ser gac Asp gtg Val gca Al a cat Hi s 180 at g Met gat Asp gac Asp acc Thr ggt Gy 260 aat Asn Page 340 cgc tta gac Arg Leu Asp 280 ggc gta gat cca Sy Val Asp Pro seql i st cor r ect ed. t xt g atg act att gca gat I IVet Thr Ile Ala Asp 5 290 gt a cag act Val G n Thr gac acc Asp Thr 295 gaa act gcg gaa G u Thr Ala G u gtc Val 300 ctc ttc ccc cgc Leu Phe Pro Arg gat ctc cca gca Asp Leu Pro Ala act Thr 310 gcc acc caa gaa Ala Thr G n G u ccc att ggt gct Pro lie Gy Ala ggc G3y 320 acc atc agc cgg Thr Ile Ser Arg gtt ccc acc atc Val Pro Thr Il e acg Thr 330 ttg aat ggc cga Leu Asn Gy Arg tac acc act gtc Tyr Thr Thr Val ttt gat Phe Asp 340 gtg ttg ctc Val Leu Leu cac tac ggt gtg Hi s Tyr G y Val aac Asn 350 cgc gaa gag ct c Arg G u G u Leu aac ctt cct Asn Leu Pro 355 act cct gcg Thr Pro Al a ggt gag tgg cct aag gat ttc cag G y G u Trp Pro Lys Asp Phe G n 360 365 gat cca gtc atg Asp Pro Val IVet ggt G3y 370 tgg cag Trp G n 375 gaa gag ctc acg G u G u Leu Thr ggt G3y 380 gtt cct gct aat Val Pro Ala Asn gcg att cgt ttg Ala Ile Arg Leu ggt Gy 390 cgg gaa ttt gct Arg G u Phe Ala aat gct gat gat Asn Al a Asp Asp t cc Ser 400 aag ggc cgt tcc Lys y Arg Ser atc atc atg ggt Ile Ile IVet y gct ggt gtg aac cac tac ttc cat gcg gat tct att Ala G y Val Asn His Tyr Phe His Ala Asp Ser lie 410 415 420 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 tat cgc aca ttc ttg gcg ctg acc tct atg tgt ggc acc caa ggt gtt Tyr Arg Thr Phe Leu Al a Leu Thr Ser Met Cys y Thr G n y Val 425 430 435 aac ggt ggc ggt tgg gct cac tac gtt ggt cag gag aaa ctc cgt cca Asn G y G y G y Trp Ala His Tyr Val G y G n G u Lys Leu Arg Pro 440 445 450 at g aat IVet Asn 455 ggt tgg gca cag Sy Trp Ala n t at Tyr 460 gcc ttt gct aca Ala Phe Ala Thr tgg cag cgt cca Trp G n Arg Pro cca Pr o 470 cgt cag atg atc Arg n IVet Ile act ggt ttc tac Thr G y Phe Tyr t ac Tyr 480 ctc acc acg gat Leu Thr Thr Asp t gg agg tat gac aac act cgt gct aat cgt Trp Arg Tyr Asp Asn Thr Arg Ala Asn Arg 490 495 ctg gct tcc cca Leu Ala Ser Pro ctg gct Leu Al a 500 aat cgt ggc Asn Arg G y t cc atg aaa Ser IVet Lys 520 gtg ggt gac aaa Val G y Asp Lys at g Met 510 acg gcg gat acc Thr Al a Asp Thr ttg gtg gaa Leu Val G u 515 aac cgc aat Asn Arg Asn cgt gga tgg atg Arg G y Trp IVet tca ttc ccg caa Ser Phe Pro G n ttc Phe 530 ccc ct c Pro Leu 535 atc ttg agc cag lie Leu Ser G n gag G0u 540 gcg gaa gaa aag Al a G u G u Lys ggc G5y 545 gtg tct gtt tct Val Ser Val Ser Page 341 gac Asp 550 cat att gtt cag His Ile Val n seql i st cor r ect ed. t xt ctc acc gat ggt gac ttg ca Leu Thr Asp G y Asp Leu G 560 g ttc gcc n Phe Al a gag gat ccg gat G u Asp Pro Asp gca Al a 570 ccg gaa aac tgg Pro G u Asn Tr p cca Pr o 575 cgc att ctg ctt Arg lie Leu Leu aac tgg Asn Trp 580 ttc ttg Phe Leu cgc aca aac Arg Thr Asn cgc cat at g Arg His IVet 600 atg ggc tct tca Vet G y Ser Ser gct Al a 590 aag ggc acg gag Lys G y Thr G u ttg ggt gtg gat Leu y Val Asp gat gca t ct gct Asp Ala Ser Ala gaa G1u 610 gaa aac gcg G u Asn Ala ccg gag Pro G u 615 gat cgt cca agt Asp Arg Pro Ser tcc Ser 620 att gtg tgg agg Ile Val Trp Arg gag gct ccg gaa G u Ala Pro G u gga Gy 630 aag ctc gat ttg Lys Leu Asp Leu ctg acc acg gat Leu Thr Thr Asp ttc Phe 640 cgc aac act t cc Arg Asn Thr Ser acc ttg gtc tcg Thr Leu Val Ser gat Asp 650 atc gtg ctg ccg Ile Val Leu Pro gca Al a 655 gcc acc tgg tat Ala Thr Trp Tyr gag aag G u Lys 660 cat gat ttg Hi s Asp Leu gct gcg at c Al a Al a I le 680 acc acg gat atg Thr Thr Asp IVet cac Hi s 670 ccc ttc atc cac Pro Phe Ile His tcg ttc aat Ser Phe Asn 675 gag gtc ttc G u Val Phe 1795 1843 1891 1939 1987 2035 2083 2131 2179 2227 2275 2323 2371 2419 2467 2503 aac cca ccg tgg Asn Pro Pro Trp acg cgt act gac Thr Arg Thr Asp tgg Tr p 690 cac gat His Asp 695 ctc acc aaa gaa Leu Thr Lys G u ttc Phe 700 t cc tca cag gca Ser Ser G n Ala gca Al a 705 acc tgg ttg ggc Thr Trp Leu y acc Thr 710 caa acc gat gtg G n Thr Asp Val acc gca ccg att Thr Ala Pro Ile gcc Al a 720 cat gac tcc ccg His Asp Ser Pro gag ctc aat atg G u Leu Asn IVet cct Pr o 730 ggc ggt atc gtg Gy y Iy Ile Val cca Pr o 735 gat att gat gag Asp Ile Asp G u gtc ggg Val G y 740 ct g at c cct Leu Ile Pro t ac t cc aag Tyr Ser Lys 760 aag acg atg gcc Lys Thr Met Ala aag Lys 750 atc atc ccg gt g Ile Ile Pro Val gaa cgt gat Su Arg Asp 755 ct c acc gcc Leu Thr Al a gtg tat gaa aag Val Tyr G u Lys aca cac ttg gga Thr His Leu G y cca Pr o 770 aaa gcg Lys Al a 775 ggt acc gga acc G y Thr G y Thr cac Hi s 780 ggc act gcg ttt G y Thr Al a Phe aac Asn 785 gtg acc aag caa Val Thr Lys G n acc Thr 790 gag gag ctg gcg G u G u Leu Al a atc aac ggc acc I e Asn G y Thr tcc atc Ser Ile 800 <210> 278 <211> 801 <212> PRT Page 342 seqi i st car r ect ed. t xt <21 3> Cor ynebact er i urn gi ut anm cumn <400> 278 Val Leu A a Ty r O31u Ty r O31n ValI Pr a Ty r Ar g Ly s 145 As p A a Pr a ValI Le u 225 Pr a ValI G31y A a Ph e 305 Th r Th r Ser vb t Pr a Ser Le u Th r Ser 115 Pr a ValI Tr p G31y Ph e 1 95 Le u A a A a Pr a Tr p 275 ValI Le u Ser ValI Trp Arg Asp O31n Ar g G31y O3 u I I e A a As p Ar g I Ie Ty r 03 y A a 70 Ser Phe Ty r Phe 03 y As p 1 00 Ser Ty r Leu As p Ser Hi s Val Val Ser 150 Ala Ar g I Ie 1 65 Hi s Val I Ie 1 80 l'b t As p Ty r As p 03 u Hi s As p Ar g Ala 230 Thr Hi s Ar g 245 G31y G31y Thr 260 Asn Leu Arg O3 n Thr Asp Pr o Ala Thr 310 Ar g G31y Val 325 Phe Asp Val 340 I Ie Lys5 A a Hi s G31y As p O31n vb t Ph e 135 Pr a Hi s Le u vb t G31y 215 A a Le u ValI Le u Thr 295 A a Pr a Le u ValI G31y A a 40 G31y Thr Tr p Thr vb t 120 vb t As p Pr a Lys5 Ar g 200 As p As p Le u A a As p 280 O31u Thr Thr Le u O31u G31y A a Ph e Ar g Tyr As p 1 Tr p ValI Ph e G31y O31u 1 Lys5 Gy le ValI As p 265 G31y Thr O31n I Ie A a 345 Thr 10 Le u Hi s Thr Ph e A a 90 ValI O31y O31u A a Thr 1 70 Ph e Tyr Thr Ser Le u 250 Ar g ValI A a O31u Thr 330 Pr a I Ie ValI ValI Le u 75 As p Pr a Ser A a As p 1 55 As p Hi s Thr Tyr Pr a 235 O31n Tr p As p O31u O31y 315 Le u Lys5 ValI Thr Pr a vb t Pr a Ser I Ie 125 Tyr Thr A a As p Ser 205 Pr a Le u As p O31u ValI 285 Le u I Ie O31y Lys5 Tyr Ar g vb t O31y A a As p ValI O31y Ph e A a 1 Ly s Ph e Ly s A a Ser 255 O31y Thr Pr a A a Ly s 335 A a O31u O31n Ser O31y Ser Tr p Thr Thr A a 1 Ph e Thr Le u Ph e Thr 240 ValI vb t I Ie Ar g O31y 320 Ty r His Tyr Oy Val Asn Arg cOu 350 Page 343 seql i st cor r ect ed. t xt G u Leu Asn Leu Pro G y G u 355 Pro Lys Asp Phe G n Asp Pro Val 365 lbet G n 385 Lys Hi s Sy G u Asp 465 Leu Al a Asp G n Sy 545 Leu SIle Thr Al a Asp 625 Ar g Thr SIle Asp Thr SIle Ar g Asp G n 435 Leu G n Thr Pr o Leu 515 Asn Ser Phe Leu Phe 595 G u Al a Thr Tyr Ser 675 G u Al a Leu G n 405 I le Val Pr o Pr o G n 485 Al a G u Asn Ser Cys 565 Tr p Leu Al a G u Thr 645 Lys Asn Phe Tr p Sy 390 I le Tyr Asn Mbet Pr o 470 Tr p Asn Ser Pr o Asp 550 G u Ar g Ar g Pr o Sy 630 Thr Hi s Al a Hi s G n 375 Ar g SIle Ar g Sy Asn 455 Ar g Ar g Ar g lbt Leu 535 Hi s Asp Thr Hi s G u 615 Lys Leu Asp Al a Asp 695 G u Phe Sy Phe 425 Sy Tr p lb t Asp Thr 505 Ar g Leu Val Asp Leu 585 Leu Ar g Asp Ser Ser 665 Asn Thr Leu Al a Al a 410 Leu Tr p Al a SI e Asn 490 Val Sy Ser G n Al a 570 let Sy Pr o Leu Asp 650 Thr Pr o Lys Thr G n 395 Sy Al a Al a G n Thr 475 Thr Sy Tr p G n G n 555 Pr o Sy Val Ser let 635 I le Thr Pr o G u Val Al a Asn Thr Tyr 445 Al a Sy Al a Lys Pr o 525 Al a Thr Asn Ser Ser 605 I le Thr Leu lbet G u 685 Ser Al a Asp Tyr 415 lbt Sy Al a Tyr Ar g 495 Thr Phe G u Sy Pr o 575 Lys Al a Tr p Asp Al a 655 Pr o Ar g G n Ala Thr Trp Leu y Thr G n Thr Asp Val Ile Thr Ala Pro Ile Ala Page 344 705 Hi s As p I I e As p Pr o Val G31y Pr o 770 As n Val 785 li1e Ser Pr o Asp 725 31u Val G31y 740 O31u Ar g As p 755 Leu Thr A a Thr Lys (3 n Le u I Ie Ser A a 775 O31u seqI i st car r ect ed. t xt 715 As n I'bt Pro Gy Gy lieVal Pr o 730 735 Pro G31y Lys Thr Wt Ala Lys I Ie 745 750 Lys Val Ty r O31u Lys Tr p Thr Hi s 760 765 G31y Thr G31y Thr Hi s G31y Thr Ala 780 G3 u Leu A a Leu I I e As n (3 y Thr 795 <210O> 279 <21 1> 765 <212> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (85)..(765) <223> FRXA01307 <400> 279 t t ccccaacc cgcat ccgct acccat acat cggt ggcgt g ct agt t gat a t gt ccgcgaa gccaaggaac gcctgggcga tccg gtg ctg gcg tgg cgc gac att gta gaa acc 114 Val Leu Ala Trp Arg Asp lieVal Ou Thr 1 5 cca Pr o at c I Ie gt g ValI gt t ValI tt g Le u g ac As p ccg Pr o t cc Ser aag Ly s gt t ValI ac c Thr ccc Pr o at g vb t cca Pr a t ct Ser at t I Ie aaa Ly s t at Tyr cgc Ar g at g G31y gca A a g ac As p gt g ValI gca A a gag O31u caa O31n t cg Ser gga G31y 80 t ca Ser t gg Tr p acc Thr t at Tyr g aa O31u t ac Tyr c ag O31n gt g ValI cca Pr a t ac Tyr cgc Ar g gt a ValI gcc A a ggc G31y 50 gt g ValI gcg A a c aa O31n aac As n ac g Thr t cc cag cgg Ser O31n Ar g 20 at g gag at t 'b t O31u Ilie ccc gac cgc Pr o Asp Ar g t ct t ac ggt Ser Tyr 03 y ct g t cc tt c Leu Ser Phe act t tc ggc Thr Phe O31y 1 00 t cc agc t ac Ser Ser Tyr 115 cct gac t cc Pr o Asp Ser aaa Ly s gcg A a cat Hi s ggt O31y g at As p c aa O31n at g vb t ggt O31y gca A a gga O31y act Thr t gg Tr p act Thr at g vb t 120 ggc O31y gcc A a tt c Ph e cgc Ar g t ac Tyr g ac As p 1 t gg Tr p cac t tc at g gt g gaa Hi s Phe I'bt Val O31u Page 345 sec 125 130 gcc cgc tac aag ggc acc aag gtt g Ala Arg Tyr Lys y Thr Lys Val 140 145 gac tcc acc aaa ttt gct gat gaa t Asp Ser Thr Lys Phe Al a Asp G u T 155 160 gac ggc gca ctc gcc ttt gcc atg g Asp Gy Ala Leu Ala Phe Ala Met C 175 cat gtt gac aag aag acg ccg tac t His Val Asp Lys Lys Thr Pro Tyr F 190 1 acg gac tct cct ttc ctc gtg gaa t Thr Asp Ser Pro Phe Leu Val G u L 205 210 tac acc cca ggt aaa ttc ctc act g Tyr Thr Pro y Lys Phe Leu Thr A 220 225 <210> 280 <211> 227 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 280 Val Leu Ala Trp Arg Asp Ile Val C 1 5 Tyr Val Ser n Arg G y Lys Gy C Su Ala Met Ou Ile Ala Ala Ala A 40 Tyr G y Pro Asp Arg lie His G y F G n Val Ser Tyr y Al a y Thr A 70 Val Ala Leu Ser Phe Tyr Asp Trp T Pro G n Thr Phe y Asp G n Thr A 100 1 Tyr Asn Ser Ser Tyr Leu IVet IVet T 115 120 Arg Thr Pro Asp Ser His Phe Iet 130 135 Lys Val Val Val Val Ser Pro Asp F 145 150 Asp u Trp Ala Arg lie His Pro C 165 Al a M\t G y Hi s Val Ile Leu Lys C 180 1 1 i st cor r ect ed. t xt 135 itt gtg gtt tcc ccg (al Val Val Ser Pro 150 gg gca cgc atc cac rp Ala Arg Ile His 165 gc cat gtg atc ttg Sy His Val Ile Leu 180 tc atg gac tac at g 'he IVet Asp Tyr IVet ta gat gag cac ggc .eu Asp G u Hi s G y 215 ca l a ttc Phe ggt Sy gaa G u 185 aaa Lys ggc Sy Su ay ~l a 'he r g yr sp rp (al 'he 8y Su Pr o SIle Val Val Leu 75 Asp Pr o Ser Al a Asp 155 Asp Lys Val Thr Pr o et Pr o Ser SIle 125 Tyr Thr Al a Lys Al a Tyr G u Arg G n bet Ser G y G y Al a Ser Asp Trp Val Thr G y Thr Phe Al a 160 Al a Phe 175 Lys Thr Phe His Val Asp Page 346 seql i st cor r ect ed. t xt Pro Tyr Phe IVet Asp Tyr IVet Arg Lys Tyr Thr Asp Ser Pro Phe Leu 195 200 205 Val G u Leu Asp G u His Gy Asp y Thr Tyr Thr Pro G y Lys Phe 210 215 220 Leu Thr Al a 225 <210> 281 <211> 1206 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (1)..(1206) <223> FRXA01308 <400> 281 gcc Al a 1 ggc Gy gaa G u at g Met ttc Phe ggt Gy cga Ar g aac Asn gat Asp cct Pr o 145 gat Asp cac Hi s act Thr gtt Val at g Met at t SIle cgc Ar g ggc Gy t ac Tyr gaa G u 115 gt c Val aat Asn t cc Ser ttc Phe aat gcc acc cac cgt ctc ctt gtg ctg caa aaa gat Asn Ala Thr His Arg Leu Leu Val Leu G n Lys Asp 5 10 gat ccc ggt ggc act gtc gcg gac cgt tgg ggt gaa Asp Pro Gy y Thr Val Ala Asp Arg Trp Gy u aag tgg aat ctg cgc tta gac ggc gta gat cca gtg Lys Trp Asn Leu Arg Leu Asp Gy Val Asp Pro Val 40 gat gta cag act gac acc gaa act gcg gaa gtc ct c Asp Val G n Thr Asp Thr G u Thr Ala G u Val Leu gat ctc cca gca act gcc acc caa gaa ggc ccc att Asp Leu Pro Ala Thr Ala Thr Gn Gu Gy Pro lie 70 75 atc agc cgg ggc gtt ccc acc atc acg ttg aat ggc Ile Ser Arg y Val Pro Thr Ile Thr Leu Asn y 90 act gtc ttt gat gtg ttg ctc gca cac tac ggt gtg Thr Val Phe Asp Val Leu Leu Ala His Tyr G y Val 105 110 ctc aac ctt cct ggt gag tgg cct aag gat ttc cag Leu Asn Leu Pro G y G u Trp Pro Lys Asp Phe G n 120 125 ggt act cct gcg tgg cag gaa gag ctc acg ggt gtt Sy Thr Pro Ala Trp n u u Leu Thr y Val 135 140 gcg att cgt ttg ggt cgg gaa ttt gct cag aat gct Ala I e Arg Leu y Arg G u Phe Ala G n Asn Ala 150 155 160 ggc cgt tcc cag atc atc atg ggt gct ggt gtg aac Sy Arg Ser n Ile Ile Iet y Al a y Val Asn 165 170 175 gcg gat tct att tat cgc aca ttc ttg gcg ctg acc Ala Asp Ser Ile Tyr Arg Thr Phe Leu Ala Leu Thr Page 347 seql i st cor r ect ed. t xt tct atg tgt Ser Vet Cys 195 ggc acc caa ggt G y Thr G n G y 185 aac ggt ggc ggt Asn G y Gy y tgg Tr p 205 190 gct cac tac Al a Hi s Tyr gtt ggt Val G y 210 cag gag aaa ct c G n G u Lys Leu cgt Ar g 215 cca atg aat ggt Pro IVet Asn y gca cag tat gcc Ala G n Tyr Ala ttt Phe 225 gct aca gac tgg Ala Thr Asp Trp cgt cca cca cgt Arg Pro Pro Arg cag G n 235 atg atc acc act bet II e Thr Thr ttc tac tac ctc Phe Tyr Tyr Leu acc Thr 245 acg gat cag tgg Thr Asp G n Trp tat gac aac act Tyr Asp Asn Thr cgt gct Arg Ala 255 aat cgt ct g Asn Arg Leu atg acg gcg bet Thr Al a 275 t cc cca ctg gct Ser Pro Leu Ala aat Asn 265 cgt ggc acc gtg Arg y Thr Val ggt gac aaa G y Asp Lys 270 tgg atg ccg Trp Met Pro gat acc ttg gtg Asp Thr Leu Val gaa G u 280 t cc atg aaa cgt Ser IVet Lys Arg gga Sy 285 tca ttc Ser Phe 290 ccg caa ttc aac Pro G n Phe Asn cgc Ar g 295 aat ccc ctc atc Asn Pro Leu Ile agc cag gag gcg Ser n G u Ala gaa G u 305 gat Asp gaa aag ggc gtg G u Lys G y Val ggt gac ttg cag G y Asp Leu n 325 gtt tct gac cat Val Ser Asp Hi s at t Ile 315 gtt cag cag ctc Val n G n Leu ttc gcc tgc gag Phe Ala Cys G u ccg gat gca ccg Pro Asp Ala Pro gaa aac Su Asn 335 tgg cca cgc Trp Pro Arg gct aag ggc Al a Lys G y 355 ctg ctt aac tgg Leu Leu Asn Trp cgc Ar g 345 aca aac cta atg Thr Asn Leu IVet ggc tct tca G y Ser Ser 350 gtg gat tct Val Asp Ser 912 960 1008 1056 1104 1152 1200 1206 acg gag ttt ttc Thr G u Phe Phe cgc cat atg ttg Arg His IVet Leu ggt 35y 365 gat gca Asp Al a 370 t ct gct gaa aaa Ser Ala G u Lys aac Asn 375 gcg ccg gag gat Ala Pro G u Asp cca agt tcc att Pro Ser Ser Ile gt g Val 385 tgg agg gat gaa Trp Arg Asp G u gct Al a 390 t cc gaa ggg aag Ser G u G y Lys ct c Leu 395 gat ttg atg ctg Asp Leu IVet Leu acg gat Thr Asp <210> 282 <211> 402 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 282 Ala Ala Thr Pro Asn Ala Thr His Arg 1 5 Leu Leu Val Leu G n Lys Asp 10 Page 348 seqI i st car r ect ed. t xt Oy Ser Val Val Asp Pro Oy Oy Thr Val Ala Asp Arg Trp O31y O31u O31u vb t Ph e O31y Ar g As n As p Pr o 145 As p Hi s Ser ValI Ph e 225 Ph e As n vb t Ser O31u 305 As p Tr p A a As p vb t li1e Ar g O31y Tyr O31u 115 ValI As n Ser Ph e cys 1 95 O31n Thr Tyr Le u A a 275 Pr o Ly s As p Ar g O31y 355 Ser Ly s As p As p I Ie Thr Le u O31y A a O31y 1 65 A a Thr Ly s Tr p Thr 245 Ser Thr Ph e ValI O31n 325 Le u O31u (31u As n O31n Pr o Ar g Ph e Le u Pr o 135 Ar g Ser Ser G31y Ar g 215 Ar g As p Le u ValI Ar g 295 ValI A a As n Ph e As n 375 Ar g As p Thr ValI ValI 1 05 G31y Tr p Gy le Tyr 1 85 As n vb t Pr a Tr p As n 265 Ser Pr a As p (31u Ar g 345 Ar g Pr a As p (31u Thr 75 Thr Le u Tr p (31u (31u 1 55 vb t Thr G31y G31y O31n 235 Tyr O31y Ly s I Ie I Ie 315 Pr a As n vb t ValI A a O31u Thr Hi s Ly s 125 Le u A a A a Le u Tr p 205 A a I Ie As n ValI O31y 285 Ser O31n A a vb t O31y 365 As p O31u O31y Le u Tyr 110 As p Thr O31n O31y A a 1 A a O31n Thr Thr O31y 270 Tr p O31n O31n Pr a O31y 350 ValI Pr a ValI Pr a As n O31y Ph e O31y As n ValI 1 Le u Hi s Tyr Thr Ar g 255 As p vb t O31u Le u O31u 335 Ser As p ValI Le u I Ie O31y ValI O31n ValI A a 1 As n Thr Tyr A a O31y 240 A a Ly s Pr a A a Thr 320 As n Se r Se r G31u Asp Ar g 380 Page 349 Pro Ser Ser Ilie seqi i st car r ect ed. t xt Val Tr p Ar g Asp (31u Ala Ser (31u G31y Lys Leu Asp Leu N/bt Leu Thr 385 390 395 400 Thr Asp <210> 283 <211> 824 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CDS <222> (801) <223> RXN01309 <400> 283 at t le cgt Ar g g ac As p ccg Pr o t gg Tr p g ac As p ggc G31y aac As n t ct Ser gag O31u 145 aac As n ggc G31y g ac As p gcc A a cgt Ar g c ac Hi s at t I Ie at c I Ie g ac As p t gg Tr p 115 t ct Ser ct c Le u gt t ValI gt g ValI g aa O31u gt g ValI t ac Ty r ct g Le u t ac Ty r at c I Ie g aa O31u at t G31y at c I Ie t ct Ser 1 65 at g vb t ggt G31y at c I Ie acc Th r t ct Ser ggc G31y 70 aat As n cca Pr o cac Hi s ggc G31y gct A a 150 gct A a aac As n ac c Th r ac c Th r gcg A a ggt G31y gag O31u ggt G31y g aa O31u t cg Ser c ag O31n 135 g ac As p cgt Ar g c ac Hi s c ac Hi s t ca Ser tt t Ph e 40 cgc Ar g c ag O31n gag O31u gt a ValI c ag O31n 120 gt g ValI aac As n gcg A a gcg A a at c I Ie ccg Pr o t cc Ser at g vb t tt g Le u gt c ValI ac c Th r 1 05 t ac Ty r at c I Ie g at As p at t I Ie c ag O31n 1 85 aat As n 10 gag O31u at c I Ie c ac Hi s cca Pr o O31y 90 gt g ValI t ac Ty r t gg Tr p t gg Tr p gt c ValI 1 70 g aa (31u t gg Tr p t gg Tr p aac As n t ac Ty r at c I Ie 75 cct Pr a cgt Ar g g ac As p at g vb t at c I Ie 1 55 t cc Ser cgc Ar g at g vb t ggt O31y g aa O31u ct c Le u ag g Ar g c ag O31n ct g Le u ct g Le u 125 aac As n gct A a cgc Ar g gct A a gt c ValI t cc Ser t ac Ty r g ac As p cca Pr a t cg Ser ac c Th r 110 cat Hi s aag Ly s t at Ty r at t O31y 1 aaa Ly s aag Ly s g ac As p c ac Hi s ccg Pr a gt c ValI ac c Th r gt g ValI g at As p aac As n cct Pr a 1 ac c Th r g aa O31u aag Ly s aag Ly s g at As p tt g Le u ac a Th r c ac Hi s ct t Le u gca A a cgc Ar g 1 g aa O31u ccg Pr a ctg aac gag aag tct ggc agg cgc ggc gga act cac aac t ct ct t act Page 350 Leu Asn Ou Lys Ser Gy Arg Ar g C 195 200 cga at c at g at t aag ccg gt c cat g Ar g I Ie I'b t I Ie Lys Pr o Val Hi s\ 210 215 acc t at ggc t tc aac t ac at c ggc c Thr Ty r G31y Phe As n Ty r I Ie G31y C 225 230 t ca cca gaa t tc gt c gcc gct ccc a Ser Pr o O31u Phe Val Ala Ala Pr o P 245 cat ggc t ca gat cgc aat gat cat g Hi s G3 y Ser As p Ar g As n As p Hi s C 260 2 cgt <210O> 284 <21 1> 267 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ami cumn Iist car r ect ed. t xt ~y G31y Th r Hi s Asn Ser Leu Thr 205 itt gcc ggt ggc t ac ggc cac t ta 672 (al Ala 31y G31y Tyr G31y Hi s Leu 220 aa ccg gaa ata acc gcg atg agg 720 ~n Pr o O31u Ilie Thr Ala I'b t Ar g 235 240 .gg agg t gc agt act aat gaa ggt 768 ~rg Ar g Cys Ser Thr Asn O31u G31y 250 255 iaa ct t gga t aagt gcat t ggct gccaca 821 Su Leu G3 y 824 <400> 284 1 1 e A a Asp His Ou Gy Thr His Ilie Ar g As p Pr o Tr p As p G31y As n Ser O31u 145 As n G31y Le u A a Ar g Hi s I Ie I Ie As p Tr p 115 Ser Le u ValI ValI O31u 1 95 ValI Tyr Le u Tyr I Ie O31u I Ie G31y I Ie Ser 1 65 vb t Ser I Ie Thr Ser G31y 70 As n Pr o Hi s G31y A a 150 A a As n G31y Thr A a G31y O31u G31y O31u Ser O31n 135 As p Ar g Hi s Ar g Ser Ph e 40 Ar g O31n O31u ValI O31n 120 ValI As n A a A a Ar g 200 Pr o Ser vb t Le u ValI Thr 1 Tyr I Ie As p I Ie O31n 1 (31y Tr p Tr p As n Tyr I Ie 75 Pr o Ar g As p vb t I Ie 1 55 Ser Ar g Thr vb t O31y O31u Le u Ar g O31n Le u Le u 125 As n A a Ar g A a As n 205 Lys5 Lys5 As p Hi s Pr a ValI Thr ValI As p As n Pr a 1 Thr Le u O31u As p Le u Thr Hi s Le u A a Ar g 1 O31u Pr a Th r Arg Ilie I'bt Ilie Lys Pro Val His Val Ala O1y Oy Tyr Oy His Leu Page 351 210 Thr Tyr G y Phe Asn 225 Ser Pro Gu Phe Val 245 His G31y Se r Asp Ar g 260 seqi i st car r ect ed. t xt 215 220 Ty r I Ie 31y O31n Pro O31u lieThr Ala l'bt Arg 230 235 240 Al a Al a Pro Arg Ar g Cys Ser Thr Asn G31u G31y 250 255 Asn Asp Hi s G3 u Leu G3 y 265 <210O> 285 <21 1> 692 <212> DNA <21 3> Cor ynebact er i urn gi ut anri cumn <220> <221 CDS <222> (669) <223> FRXA01309 <400> 285 at t le cgt Ar g g ac As p ccg Pr o t gg Tr p g ac As p ggC G31y aac As n t ct Ser gag (31u 145 aac As n g ac As p gcc Al a cgt Ar g c ac Hi s at t I Ie at c I Ie g ac As p t gg Tr p 115 t ct Ser ct c Le u gt t ValI g aa (31u gt g ValI t ac Ty r ct g Le u t ac Ty r at c I Ie g aa (31u at t G31y at c I Ie t ct Ser 1 65 ggt G31y at c I Ie acc Th r t ct Ser ggc G31y 70 aat As n cca Pr o cac Hi s ggc G31y gct Al a 150 gct Al a ac c Th r ac c Th r gcg Al a ggt G31y gag (31u ggt G31y g aa O31u t cg Ser c ag O31n 135 g ac As p cgt Ar g c ac Hi s t ca Ser tt t Ph e 40 cgc Ar g c ag O31n gag O31u gt a ValI c ag O31n 120 gt g ValI aac As n gcg Al a at c I Ie ccg Pr o t cc Ser at g vb t tt g Le u gt c ValI ac c Th r 1 t ac Ty r at c I Ie g at As p at t I Ie t gg Tr p t gg Tr p aac As n t ac Ty r at c I Ie 75 cct Pr o cgt Ar g g ac As p at g vb t at c I Ie 1 55 t cc Ser at g vb t ggt G31y g aa O31u ct c Le u ag g Ar g c ag O31n ct g Le u ct g Le u 125 aac As n gct Al a cgc Ar g aaa Lys5 aag Lys5 g ac As p c ac Hi s ccg Pr a gt c ValI ac c Th r gt g ValI g at As p aac As n cct Pr a 1 g aa O31u aag aag g at As p tt g Le u ac a Th r c ac Hi s ct t Le u gca Al a cgc Ar g 1 g aa O31u ggc acc gtg ttt atg aac cac gcg cag gaa ccc acc gct ggc acc ccg Page 352 (31y Th r Val Phe [Vbt Asn Hi s 1 80 ctg aac gag aag tct ggc agg Leu Asn O31u Lys Se r O31y Ar g 1 95 cga at c at g at t aaa acg gt c Arg Ilie 'b t Ilie Lys Thr Val 210 215 t aacct at gg ct taact aca t cg <210> 286 <211> 223 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ar seqI i st car r ect ed. t xt Ala O31n 31u Pro Th r Ala (31y Th r 185 190 cgc ggc gga act cac aac tct ctt Ar g 31y G31y Th r Hi s Asn Ser Leu 200 205 cat gt t gcc ggt ggc tac ggc act Hi s Val Ala 31y G31y Tyr G31y Thr 220 <400> 286 1 1 e A a Asp Ar g Ser Ala Asp G31y Ar g Pr o Tr p Hi s Tr p Phe I Ie As p Lys I I e 03 y Thr As p As n Lys Tr p 115 Ser I Ie Ser 130 03 u Lys Leu 145 Asn G31y Val 03 y Thr Val Leu Asn 03 u 1 95 Arg Ilie Nib t 210 Hi s 0 u 0yThr O31u Val I IIe Thr Ar g Ty r Thr Ala Thr Leu Ser 03 y As p Ty r 03 y 03 u 70 Hi s I I e As n 03 y G31y 031u Pr o 031u 1 00 Ser I Ie Hi s Ser Ar g G31y 31y O31n 135 O3 y I I e A a As p 150 Val Ser A a Ar g 1 65 Phe I'b t As n Hi s 1 80 Lys Ser G31y Ar g Hi s I I e As n 10 Ser Pro O31u Phe Ser I Ie 40 Ar g I'b t Hi s O31n Leu Pr o 31u Val G31y 90 Val Thr Val 1 O3 n Ty r Ty r 120 Val I Ie Tr p As n As p Tr p A a I I e Val 1 70 A a 03 n 03 u 1 Arg G31y G31y 200 Tr p Tr p As n Tyr I Ie Pr o Ar g As p vb t I Ie 1 Ser Pr o Thr vb t G31y O31u Le u Ar g O31n Le u Le u 125 As n A a Ar g A a As n 205 As p Hi s Pr o ValI Thr ValI As p As n Pr a 1 Thr Le u I I e Lys Thr ValI 215 His Val Ala O1y Ty r 03 y Th r <210O> 287 <21 1> 807 <212> DNA <21 3> Cor ynebact er i urn gi ut anm curn Page 353 seqi i st car r ect ed. t xt <220> <221> ODS <222> (101)..(784) <223> RXA02017 <400> 287 gcctgtggca cgggagctcc cagcggcaag gtgagtctga cgccccgctg cgatgttccc accaaggaag gactaggcgg cct cgt ggaa cgt t ggcgaa ggc O31y cag O31n gag O31u gt c Val gca Al a ttt Phe ggc O31y ct g Leu ct C Leu gac Asp 150 ttg Leu at g lvb t agc Ser at c l Ie at t l Ie cgc Ar g gga O31y ccg Pr o gt c Val cag O31n acc Thr cag O31n 120 gt c Val gcc Al a gca Al a ttg Leu at g lvb t 200 acg Thr gat Asp gt g Val gt c Val ccc Pr o ggg G31y cga Ar g cag O31n gga O31y ct t Leu cag O31n ct C Leu 170 cag O31n ct C Leu ct a Leu cac Hi s tt C Phe gac Asp gt c Val ct a Leu 75 cgc Ar g cgc Ar g ttt Phe gag O31u gt g Val 155 gac Asp gca Al a at c l Ie ccg Pr o ttt Phe at g lvb t aag Lys gcg Al a cgc Ar g t gc Cys ggc O31y gaa O31u gcc Al a 140 tta Leu aac Asn ttg Leu cgc Ar g ttc Phe 220 gt g Val ct g Leu ct a Leu 45 gcg Al a gcc Al a t ca Ser acg Thr t cc Ser 125 gca Al a t ca Ser ct C Leu cca Pr o agc Ser 205 ccc Pr o cct Pr a aat Asn aat Asn cac Hi s at g lvb t ct g Leu gcg Al a 110 gag O31u gcg Al a gct Al a gac Asp gaa O31u 190 ggt O31y acc Thr cgc Ar g 15 agt Ser gcc Al a gt g Val cag O31n ttt Phe 95 at c l Ie cgc Ar g ct t Leu cac Hi s t cg Ser 175 at c l Ie cca Pr a t ca Ser at c l Ie ttg Leu gt c Val gt c Val gaa O31u 80 ct C Leu ct C Leu gac Asp gct Al a cgc Ar g 160 ccc Pr a gat Asp cca Pr a caa O31n at g lvb t 1 t cc Ser ct g Leu gag O31u gag (31u gcc Al a acc Thr acc Thr gaa O31u gat Asp 145 gac Asp t ac Tyr gaa O31u gca Al a ccg Pr a 225 cgc acc cat Arg Thr His at g lvb t ttg Leu gcg Al a ttc Phe t ac Tyr t ac Tyr ttc Phe ttg Leu 130 t ct Ser ggc O31y aga Ar g gaa O31u gaa O31u 210 gac Asp gag O31u t at Tyr ct t Leu gac Asp gag O31u gct Al a 100 caa O31n gac Asp ct t Leu gaa O31u ct g Leu 180 gcc Al a gt g Val cac Hi s act Thr gag O31u cca Pr a gat Asp gcg Al a acc Thr gt g Val acg Thr cac Hi s ttc Phe gt g Val 165 at c l Ie aac Asn ggc O31y Page 354 ^Q seql i st cor r ect ed. t xt St aggacacac t at gt caaac t t t 807 <210> 288 <211> 228 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 288 IVet Arg Thr His Thr G y Lys IIe Pro Asp His Phe Val Pro Arg I e 1 5 10 CK Ser Met Thr G u G u G n Arg Arg Val Val Phe IVet Leu Asn Ser Leu 25 00 0 Leu Leu Asp Tyr Pro G u G u y Phe Val Asp Lys Leu Asn Ala Val 40 O G u Ala G n Leu Asp Val Leu Pro Leu Pro Val Ala Ala His Val Val 55 C G u Phe Leu Asp Al a Al a Arg Val Al a y Leu Arg Al a IVet G n u 70 75 Ala Tyr Val G u Thr Phe Asp G n Arg Arg Arg Cys Ser Leu Phe Leu 90 Thr Tyr Tyr Ala Val G y Asp Thr Arg G n Arg y Thr Ala lie Leu 100 105 110 Thr Phe Arg G n Thr Leu n G n Leu y Phe u Ser G u Arg Asp 115 120 125 G u Leu Pro Asp His Leu Cys Val Val Leu G u Ala Ala Ala Leu Ala 130 135 140 Asp Ser Ser Leu Phe Asp Ala Ala Thr G n Val Leu Ser Ala His Arg 145 150 155 160 Asp y ile G u Val Leu Arg Ala Ala Leu Asp Asn Leu Asp Ser Pro 165 170 175 Tyr Arg Tyr Leu Ile Iet Ser Leu Cys G n Ala Leu Pro G u Ile Asp 180 185 190 G u G u Thr Al a Asn Ser Tyr IVet G u Leu I e Arg Ser G y Pro Pro 195 200 205 Al a G u Iet Val G y Ile G y Thr Pro Leu Pro Phe Pro Thr Ser G n 210 215 220 Pro Asp Ile His 225 <210> 289 <211> 1073 <212> DNA <213> Cor ynebact er i um gl ut ai cum <220> <221> CDS <222> (1)..(1050) <223> RXA02018 <400> 289 cgc atc tgc gag cac tgc ctc aac ccc acc tgt gtg tcc tcc tgc cca 48 Arg lie Cys G u His Cys Leu Asn Pro Thr Cys Val Ser Ser Cys Pro Page 355 seql i st cor r ect ed. t xt t cc Ser cag G n aaa Lys ct g Leu acg Thr gac Asp gaa G u gcc Al a cag G n 145 ccg Pr o cca Pr o gaa G u ct g Leu gaa G u 225 at c l Ie at g Met aag Lys gct Al a caa G n gt c Val t at Tyr gt g Val gt c Val caa G n 115 gcc Al a tct Ser cac Hi s agc Ser cac Hi s 195 t ac Tyr t cc Ser ttg Leu ggc Sy gac Asp t ac Tyr cgt Ar g ttc Phe cgc Ar g cgc Ar g gaa G u acc Thr cgc Ar g at t SIle gaa G u 165 at c SIle at c SIle gct Al a cga Ar g cgc Ar g 245 gt g Val cgc Ar g aaa Lys ggc Sy aat Asn ct c Leu 70 tt g Leu gt c Val ct c Leu aac Asn tac Tyr 150 tac Tyr gtt Val ct g Leu gga Sy cgc Ar g 230 gaa G u gt g Val t at Tyr cgc Ar g tgg Tr p cac Hi s gag G u cgc Ar g gcc Al a ttc Phe ggc G y 135 gat Asp cgc Ar g gat Asp ct c Leu ttg Leu 215 ct a Leu cct Pr o cag G n gt c Val gcc Al a cgc Ar g 40 aaa Lys gt c Val t ac Tyr gcc Al a ct a Leu 120 at c SIle ct c Leu acc Thr gag G u acc Thr 200 ttc Phe gcc Al a cag G n gaa G u at c gaa G u at g Met tcg Ser ggc Sy ttg Leu acg Thr 105 gat Asp ccg Pr o at c SIle ttg Leu gt g Val 185 gcg Al a act Thr gcc Al a gaa G u at g Met 265 ccc gac Asp tgt Cys ggc Sy cag G n ggc Sy 90 cca Pr o ccc Pr o cac Hi s ttc Phe ccg Pr o 170 acc Thr ct g Leu gcc Al a at g Met gaa G u 250 t at Tyr ggc G y gtt Val aag Lys ccg Pr o 75 gtt Val gac Asp cac Hi s t cc Ser aaa Lys 155 at g Met gcg Al a t cc Ser ggt Sy cga Ar g 235 at c SIle cgc Ar g at c SIle t cc Ser gcc Al a acc Thr ttg Leu gaa G u gac Asp tgg Tr p 140 t ac Tyr gtt Val t cc Ser acc Thr gat Asp 220 t ca Ser gca Al a at c SIle gt g Val ggc Sy gaa G u gt g Val ctt Leu aag Lys cca Pr o 125 ct c Leu gag G u tgg Tr p ggc G y at g Met 205 acc Thr t at Tyr gag G u ct g Leu ct g Leu tgc Cys aag Lys tgc Cys t ac Tyr gat Asp 110 cag G n gaa G u gtt Val t ac Tyr aac Asn 190 cgc Ar g agg Ar g at g let gct Al a gcc Al a 270 gtt Val ccc Pr o tgt Cys t cc Ser gac Asp ctt Leu gt g Val gct Al a gcc Al a at t SIle 175 gac Asp at c SIle ccg Pr o cgc Ar g gt c Val 255 at t l Ie gac Asp t ac Tyr acg Thr gag G u gcc Al a ttc Phe at c SIe gcg Al a ct c Leu 160 ccg Pr o ggc G y ccg Pr o gtg Val gat Asp 240 gga Sy gcc Al a Ile Pro acc gcc tcc cct Thr Ala Ser Pro Page 356 gag acc ccg G u Thr Pro seqI i st car r ect ed. t xt 275 280 285 cgc gga at t t ct t cc ct g gat cct ttc ggc gat gt c gat Ar g G31y I Ie Ser Ser Leu Asp Pr o Phe G31y Asp Val Asp 290 295 300 gcc acc gag cag ctc aac atc ggt ttg ggc gaa ggc gct A a Thr G3 u G3 n Leu Asn Il e G3 y Leu G3 y G3 u G3 y A a 305 310 315 t gt ggc acg gga gct ccc agc ggc aag gt g agt ct g acc Cys a31y Thr a31y Ala Pr o Ser a31y Lys Val Ser Leu Thr 325 330 gtt ggc gaa cgc ccc gct gcg atg ttc cca cca agg aag Val a31y a31u Ar g Pr o Ala Ala I'b t Phe Pr o Pr o Ar g Lys 340 345 t aggcggat g cgcacccat a ct g <210> 290 <211> 350 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn cca Pr o cca Pr o t cg Ser g ac As p 350 gcc cga Ala Ar g gag gcc a3 u A a 320 t gg aac Tr p As n 335 912 960 1 008 1 050 1 073 <400> 290 Ar g I Ie Cys Ser a3 y A a a31n As p a31n Lys Lys Val Leu Cys Tyr Thr Cys Val Asp Ar g Val a3 u A a a3 n 115 A a Asp A a 130 a3 n Asn Ser 145 Pr o Leu Hi s Pr o Leu Ser a3 u Asp Hi s 1 95 Leu 03 u Tyr 210 a3 u Hi s Cy s I'b t Ty r Lys Cys Ar g a31y Tyr Phe Asn Pr o Ar g Leu 70 a31y Ar g Leu A a a3 u Val 1 00 Lys Thr Leu a31n Ar g Asn Pro Ilie Tyr 150 Pro a31u Tyr 1 65 Pro Ilie Val 1 80 Lys Il e Leu Leu A a (3 v Le u Ar g Tr p Hi s 55 a31u Ar g A a Ph e a31y 135 As p Ar g As p Le u Le u 215 As n A a Ar g 40 Lys5 ValI Tyr A a Le u 120 li1e Le u Thr a31u Thr 200 Ph e Pr o a31u vb t Ser a31y Le u Thr 1 As p Pr o I Ie Le u ValI 1 A a Thr Cys a31y ValI Lys5 Pr o ValI As p Hi s Ser Lys5 1 vb t A a Ser ValI I Ie Ser A a Thr Le u a31u As p Tr p 140 Tyr ValI Ser Thr Ser ValI a31y a31u ValI Le u Lys5 Pr o 125 Le u a31u Tr p a31y vb t 205 Cys ValI Pr o Cys Ser As p Le u ValI A a A a I Ie 1 As p I Ie A a 03 y Asp 220 Page 357 Thr Arg Pro Val (31u 225 li1e vb t Ly s Ar g A a 305 Cys ValI Ser Le u G31y As p 275 li1e (31u Thr (31u Ar g Ar g 245 ValI Ar g Ser Le u A a 325 Pr o Le u Pr o O31n ValI As p 295 li1e Ser A a seql i st car r ect ed. t xt Ala Ala l'b t Ar g Ser Tyr 235 03 n 03 u 03 u I I e A a 03 u 250 (31u l'b t Ty r Ar g I Ie Leu 265 li1e Pr o Thr Ala Ser Pr o 280 285 Pr o Phe G31y As p Val As p 300 03 y Leu 03 y 03 u 03 y A a 315 03 y Lys Val Ser Leu Thr 330 l'b t Phe Pr o Pr o Ar g Lys 345 vb t A a A a 270 O31u Pr o Pr o Ser As p 350 Ar g ValI 255 li1e Th r A a O31u Tr p 335 <210O> 291 <21 1> 900 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (877) <223> RXA02016 <400> 291 acat ggagct cat ccgcagc ggt ccaccag cagaaat ggt gggcat cggc acgcctct ac cgt tccccac ct cacaaccg gacat tcact aggacacact t ca aac t tt gaa Ser Asn Phe 0 u ac g Thr at c I Ie c ac Hi s ct c Le u ct t Le u gct A a ct c Le u at t I Ie t cc Ser c ac Hi s at c I Ie c ac Hi s gt t ValI t gg Tr p at c G31y aag Ly s at c I Ie gcc A a cgc Ar g t ac Tyr at g vb t ag c Ser 75 gcc A a t ac Tyr t gg Tr p g aa 031u gt g ValI t gg Tr p ac c Thr t gg Tr p gcc A a aaa Ly s gt g ValI c aa O31n cca Pr o t gt Cys c aa O31n ct c Le u at c I Ie gt a ValI ac c Thr gcc A a ggt G31y at c lIe c ac Hi s at t I Ie gcc A a gcc A a t gg Tr p gcc A a ct c Le u t ct Ser ggc G31y t ac Tyr ac c Thr t cc Ser at g vb t g ac As p at c I Ie 1 00 gca gt c ct t gga ct c at c ggc t tg at t at c cgt cgc gtg atc aac aaa Page 358 Al a Val Leu seqI i st car r ect ed. t xt Leu Ilie y Leu Ilie i~e Arg Arg Val ac c Thr ct c Le u gt c ValI 1 50 t gg Tr p g at As p at c I Ie gt t ValI ac C Thr 230 gt c ValI tt c Ph e 120 gct Al a ggc G31y cgc Ar g cct Pr o ct g Le u 200 t ac Tyr g aa O31u aat As n t cc Ser at t I Ie gca Al a ct g Le u 1 70 g aa O31u cca Pr o ac C Thr gca Al a ct c Le u 250 t ca Ser t cc Ser 140 ggC G31y at c I Ie aag Ly s ac c Thr CCC Pr o 220 C aa O31n aat As n 110 t cc Ser tt c Ph e g ac As p aac As n C ac Hi s 1 90 ct a Le u gt g ValI gt c ValI g ac As p at c I Ie t ac Tyr gct Al a 1 75 at c I Ie gt c ValI gt c ValI gcc Al a aaa Ly s gcc Al a cgc Ar g 1 60 C aa O31n gt c ValI C ac Hi s t at Tyr t gg Tr p 240 gt g ValI ac C Thr 145 g aa O31u cca Pr o gct Al a gcg Al a cgc Ar g 225 g aa O31u at g vb t 130 gt c ValI ac C Thr gag O31u gga G31y tt c Ph e 210 ac C Thr ccg Pr o I I e Asn Lys 115 t at gt g ct a Ty r Val Leu t cc acc cag Ser Thr 03 n at c t cc cca Ilie Ser Pr o 1 ct c at g gct Leu I'b t Al a 1 t tc acc ct c Phe Thr Leu 1 t cc gca cca Ser Al a Pr o cgc gac acc Ar g Asp Thr at c cgc t cg Ilie Ar g Ser gac tcg aaa Asp Ser Lys 255 t gg cac ggc gcc Trp His Gy Ala t aaat tcctc acaagccccc tag <210> 292 <211> 259 <212> PRT <213> Cor ynebact er i urn gi ut anm cumn <400> 292 I'bt Ser Asn Phe O31u Thr Phe Leu Trp 1 5 li1e AiaAliaTyr I Ie I Ie G31y I Ie Se r 25 Phe G31y Tr p Th r Thr Hi s Ser Ser O31n 40 Ar g I Ie Al a Ser Pro Leu Phe Hi s Tr p G31y Hi s Leu I'b t G31y Leu Al a Ilie Pr o 70 G31y Ilie Ser Asp Al a Al a Ty r Hi s Leu Ile Ala (3y Ile Ala AlaVal Leu (3y 100 105 Val Ala 10 Tr p Ar g I I e Ty r 31y Ib t Lys Ser 1I e Al a 90 Leu I I e Page 359 Pr a Ar g Ser Ph e Thr I Ie Le u Tr p Al a Ly s ValI O31n Pr a I Ie 110 Le u As p Le u ValI Al a G31y lie Ar g ValI Thr 145 (31u Pr a A a A a Ar g 225 G u Hi s I Ie 115 Tyr Ser I Ie Le u Ph e 1 95 Ser Ar g I Ie A a Ly s Le u O31n Pr a 1 65 A a Le u Pr a Thr Ser 245 ValI G31y 135 Ph e ValI ValI A a G31y 215 Ser Ly s seql i st car r ect ed. t xt Phe Leu Ser Thr Ser Ar g 120 125 A a A a I I e Leu Ser 03 y 140 O3 y 03 y A a Hi s 03 y Ty r 1 Ar g O31n Leu Leu I Ie Phe 1 70 Pr o Tr p O31u Phe Lys Val 1 Leu Tr p Pr o Phe Thr Ar g 200 205 Ty r Val Thr Ar g Pr o Ty r 220 O31u Pr o Ala Ar g O31n As n 235 Asn G n Leu Asp Asn Asp 250 As p I Ie Ty r A a 1 li1e ValI ValI A a Ly s 255 <210O> 293 <21 1> 813 <21 2> DNA <213> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (790) <223> RXA00471 <400> 293 acgcat caaa tct gaaat cg gcgaaggcac cgaagt ggca ggt cgt acgc gcgt gt ct t c ggggct gt aa cct gaaaggc tt g Le u ggC G31y gt a ValI c ac Hi s t cc Ser g at As p gcc A a ggc G31y ccc Pr a gt g ValI c ac Hi s ac a Thr aag Ly s ggc G31y ac c Thr t cc Ser gt a ValI gca A a ggc G31y at t lIe 75 gt g ValI gt c ValI ag c Ser ggc G31y tt c Ph e tt t Ph e ggc G31y aaa Ly s 45 ct c Le u tt g Le u cgc Ar g g aa (31u cca Pr a gca A a gca A a t cc ggc Ser 03 y 15 gca ggg A a 03 y gag gt a 03 u Val gt g ct c Val Leu ct c agt Leu Ser 80 Page 36C at caccat gg at gt gt agt t at g gt t gat gt g tt t I'b t Val Asp Val Phe 1 gtc aaa gca gaa cta Val Lys Ala O31u Leu acg gt g gcc gac gcc Thr Val Ala Asp Ala gt g ct t ct c gac gt c Val Leu Leu Asp Val cag cag atc aac gac 03 n 03 n Il e Asn Asp gt c t ct gat gct gcg Val Ser Asp Ala Ala seql i st cor r ect ed. t xt gaa gat gtc atc Gu Asp Val lie aaa t ca Lys Ser t cc ggc Ser G y gcc ttc Al a Phe 135 ccc gaa Pro G u 150 gtt gtc Val Val gcc cgc Al a Ar g gtc aaa Val Lys caa t cc G n Ser 215 atc t cc I e Ser 105 gac gca Asp Al a 120 gcc gcc Al a Al a aaa gac Lys Asp gac gcc Asp Al a ggc tac G y Tyr 185 acc gtg Thr Val 200 aac cgc Asn Arg gcc atc atc cgt Ala lie Ile Arg ggt gaa gaa ct c G y G u G u Leu ttc ttc tca cca Phe Phe Ser Pro 125 ccc gat tcc gca Pro Asp Ser Ala 140 gcc gcc gta gaa Ala Al a Val G u 155 ctc acc cgc cgc Leu Thr Arg Arg 170 acc tac aaa gaa Thr Tyr Lys G u gaa acc cac gcc G u Thr His Ala 205 cac gcg ttg acc His Al a Leu Thr uy ggc ggt gcc at c SIle 110 cgc Ar g gct Al a t cc Ser gaa G u at c SIle 190 t ca Ser cgg Ar g uy 95 gaa G u ct g Leu ggc a y gga G y ct c Leu 175 ggc G y aac Asn tgg Tr p AI a gcc Al a gca Al a gca Al a aaa Lys 160 gaa G u aaa Lys at t SIle gct Al a agg gga tac Arg G y Tyr atc aac cgc lie Asn Ar g 115 ggc ttt gtc G y Phe Val 130 ggc att gt c G y I I e Val 145 at c ct c gac Ile Leu Asp gt c ct c cgc Val Leu Arg gaa ctg ttc G u Leu Phe 195 ctg cgg aaa Leu Arg Lys 210 cac tcg agg His Ser Arg 225 gt g Val 100 gt g Val ct c Leu gac Asp gac Asp ct a Leu 180 at t SIle acc Thr gat Asp acc Thr aaa Lys gac Asp gca Al a cca Pr o 165 ct a Leu t cc Ser caa G n ctt Leu gac taatggcggc taaaaagag Asp 230 <210> 294 <211> 230 <212> PRT <213> Corynebacter i um gl <400> 294 IVet Val Asp Val Phe Leu 1 5 Val Lys Ala G u Leu y Thr Val Ala Asp Ala Val Val Leu Leu Asp Val Hi s Sn G n Ile Asn Asp Ser 70 Val Ser Asp Ala Ala u Arg G y Tyr Val Thr Lys 100 t ggc ut am cum Val Asp Asp Asn Al a Val 25 Al a G y l e 40 IVet Pro Asp Asp Val Asp Asp Val I e Ser IIe Ser 105 Hi s Ser 10 Thr Val Lys Al a G y G y Thr Ile I 75 Ala l e I 90 Gy G u i Page 361 Phe Sy Lys Leu Leu Ar g Leu Ar g G u Pr o Al a Al a Sy SIle 110 Ser Al a G u Val Leu Sy G u Sy Sy Val Leu Ser Al a Al a seql i st cor r ec Ile Asn Arg Val Lys Ser y Asp Ala Phe Phe 115 120 Sy Phe Val Leu Asp Ala Phe Ala Ala Pro Asp 130 135 Gy I e Val Asp Ala Pro u Lys Asp Ala Ala 145 150 155 Ile Leu Asp Asp Pro Val Val Asp Ala Leu Thr 165 170 Val Leu Arg Leu Leu Al a Arg Gy Tyr Thr Tyr 180 185 G u Leu Phe lie Ser Val Lys Thr Val G u Thr 195 200 Leu Arg Lys Thr n G n Ser Asn Arg His Ala 210 215 His Ser Arg Asp Leu Asp 225 230 <210> 295 <211> 936 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(913) <223> RXA00133 <400> 295 gttacatcag atgaggatgc cctatgggtg tacacatgcg aatttgaagg tggataccca gcggattaaa gatgatgaag ted. txt Ser Pro 125 Ser Al a 140 Val G u Arg Arg Lys G u Hi s Al a 205 Leu Thr 220 Ar g Al a Ser G u SIle 190 Ser Ar g Leu Gy Gy Leu 175 Gy Asn Tr p Al a Al a Lys 160 G u Lys SIe Al a acgggtgtat tgcaggagga atg cta ttc gtt cgg 11! IVet Leu Phe Val Ara cgg Ar g gcc Al a ttg Leu gtt Val t ac Tyr gat Asp aca Thr gt g Val acc Thr gga Gy agg Ar g ggc Gy ct g Leu cag G n gct Al a gt c Val aat Asn cat Hi s aaa Lys gac Asp ct g Leu gt c Val gt c Val 75 t cc Ser acc Thr aat Asn cag G n gca Al a at t SIle at t SIle gca Al a cgc Ar g aat Asn 45 acc Thr t ca Ser gt c Val aca Thr ct g Leu ct g Leu cgt Ar g cat Hi s gca Al a ggc G y 15 caa G n gt c Val cgt Ar g gag G u gtt Val 95 at c SIle at t SIle at t SIle ccg Pr o aag Lys ccc Pr o 1 cca Pr o act Thr gaa G u gtt Val gat Asp gt g Val gt c Val cag G n cca Pr o ggt G y t cc Ser at c SIle acc Thr tgg Tr p ggt G y gt g Val gcg Al a gt g Val at g let gtt Val gtg Val agt Ser att SIe cac Hi s 100 gaa att cgt ggc gtt ttg tat gtt ggc gtt cac tct gcg gtg cgt ctc Page 362 seqIi O31u I Ie Ar g O31y 1 05 Val Leu Tyr ggc O31y c aa O31n ggt G31y 1 50 gag O31u gt g ValI at g vb t gag O31u gct Al a 230 cgc Ar g act Th r 120 ct g Le u ggt G31y gt t ValI g at As p ac c Th r 200 g ac As p g aa O31u gt c ValI at t I Ie at c I Ie ct c Le u t cc Ser 1 70 g at As p gt c ValI ct g Le u at g vb t cgc Ar g 250 gaa O31u aac As n aaa Ly s 155 act Th r ct g Le u cgc Ar g gct Al a ggc O31y 235 aag Ly s g aa O31u t cc Ser 140 gct Al a cat Hi s cgc Ar g at c I Ie t gt cys 220 at c I Ie ct c Le u ValI gt c ValI 125 gcg Al a aac As n t cc Ser cgc Ar g aag Ly s 205 gt c ValI ggc G31y G31y (31y 110 ac c Th r ct t Le u cgc Ar g aag Ly s g at As p 1 90 c ag O31n gcg Al a gcg Al a gcc Al a st car r ect ed. t xt Val Hi s Ser Al aVal 115 act Th r cgc Ar g at g vb t 1 60 cgc Ar g g aa O31u ac c Th r ggt G31y ac c Th r 240 ac g Th r gcg Al a aat As n 145 aat As n at g vb t gag O31u aag Ly s c ac Hi s 225 gt c ValI cgc Ar g cgc Ar g 130 ggc G31y G31y ct g Le u ct t Le u ct g Le u 210 ac c Th r aag Ly s t ac Ty r Arg Leu ac g Th r gt t ValI gcg Al a gca Al a t gc cys 1 t cc Ser aac As n ag c Ser gag O31u t tg g Leu 0 cct g Pr o A gag t 03 u Ti 11 aat ci Asn A 1 gat c~ AspOG gcg c~ Al a A gt c g Val G t ac ci Ty r b 2, gca g I Al a V, 260 aac gca gca cgc cgg at c ggc gca ct g cct Asn Ala Ala Arg Arg Ilie Gy Ala Leu Pro 265 270 ac g <210> 296 <211> 271 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 296 I'bt Leu Phe Val Ar g Ar g Leu Thr Ser Leu 1 5 10 Pr o Val Thr I'b t Phe Al a Th r Val Leu O31n Thr O31n Tr p Val G31y Leu Arg Thr Pro Al a 40 O31u Pr o G31y Val G31y Val G31y G31y Ar g Val 55 Val G31y Val Ser Asp Tyr Thr Ar g Al a As n 70 Asp Ser Al a Il e 03 n Asp 03 u 03 y Leu Hi s Pag t aaaaagat t t tgct t tacg Lys As p Leu Val ValI Ser e 363 Th r As n Al a li1e I Ie Al a Ar g As n Thr Ser ValI O31y O31n ValI Ar g O31u ValI seqI i st car r ect ed. t xt Val Leu Tyr Val ValI Ser A a As n 145 As n vb t (31u Ly s Hi s 225 ValI Ar g ValI ValI 115 Thr ValI A a A a CYS 1 95 Ser As n Ser O31u Ar g 03 u I Ie Ar g Le u O31u As p Tr p 1 65 Ar g O31n Ar g O31u Le u 245 G31y O31n G31y 150 O31u ValI vb t O31u A a 230 Ar g As p As n 135 Ar g O31n Thr ValI Le u 215 A a Ser G31y 1 05 ValI A a Ser Ar g (31u 1 85 Pr o ValI (31u vb t Ar g 265 G31u As n Ly s 1 Thr Le u Ar g A a O31y 235 Ly s ValI 125 A a As n Ser Ar g Ly s 205 ValI O31y (31y O31y 110 Thr Le u Ar g Ly s As p 1 O31n A a A a A a ValI Ar g ValI Le u 1 Le u Thr Le u O31u Hi s 255 A a Val Asn A a A a 260 Ar g I Ie O31y Ala Leu Pro 270 <210O> 297 <21 1> 759 <212> DNA <21 3> Cor ynebact er i urn gI ut anri cumn <220> <221 CIDS <222> (736) <223> RXA00650 <400> 297 aaggct agac t aaagt acga t t cat ct gct cat cgaaacgaag tgcgccattg ggaaggacct agt cgat act ct tgaaggcg catt t tcat t tcaaaca at g at t cgc gt g ct g I'b t I Ie Ar g Val Leu 1 ct c gga ct c cga gct gt g ct g Leu O31y Leu Ar g Ala Val Leu 15 ggc gaa gt c t cc acc gcc gaa O31y O31u Val Ser Thr Ala O31u gga at c gac gt c at c t tg at g O31y Ilie Asp Val Ilie Leu I'b t gga acc cag gtt tcc aca ggc (31y Thr O31n Val Ser Thr G31y Page 364 ct t Le u g aa O31u ggt O31y g ac As p g at As p gcc A a gt g ValI cga Ar g c ac Hi s g ac As p gca A a ggc (31y gaa O31u at t I e gcc A a ccc Pr o at c I Ie g aa O31u c aa O31n (31y gt g ValI gt g ValI g aa O31u 45 gt c ValI ag g Ar g gt g ValI ggc O31y c aa (31n seql i st cor r ect ed. t xt gca Al a gt c Val at c SIle gaa G u t ca Ser acc Thr 150 gga Gy acg Thr t ca Ser gac gcc Asp Al a ctg gt c Leu Val gaa gcc u Al a ct c ct g Leu Leu 120 ccc at g Pro 'bet 135 t ca ct c Ser Leu t cc t cc Ser Ser gtg aaa Val Lys cgt acc Arg Thr 200 gca Al a acc Thr gca Al a gca Al a gcg Al a cca Pr o cgc Ar g 170 cac Hi s gct Al a gcc Al a 75 aac Asn ct g Leu gt a Val aac Asn cgt Ar g 155 gac Asp ct c Leu gt c Val atc aag cga aac atc lle Lys Arg Asn t ac Tyr ggc G y cga Ar g cgc Ar g 140 gaa G u at t SIle gt g Val gca Al a acc Thr ct g Leu 110 gca Al a at g Met gaa G u cgt Ar g at c SIle 190 gca Al a gac Asp 95 ct c Leu gca Al a act Thr gtt Val at c SIle 175 t ac Tyr cgt Ar g I e 80 aca Thr aaa Lys gaa G u cgc Ar g ct c Leu 160 ct c Leu gac Asp gag G u aac ccg Asn Pro at c ct c I e Leu gcc cca Ala Pro 115 gac t cc Asp Ser 130 cgc acc Arg Thr ctg gtt Leu Val ctc t ca Leu Ser ctc ggc Leu G y 195 ggg ctg G y Leu 210 cca Pr o ggc G y 100 ccg Pr o aca Thr ccc Pr o gcc Al a gaa G u 180 gt g Val ctg Leu aaa Lys gca Al a agc Ser ctg Leu aaa Lys ggt G y 165 gcc Al a cgg Ar g tagcgggggt tgctgcaagg ctt <210> 298 <211> 212 <212> PRT <213> Corynebacter i um gl utam cum <400> 298 IVet lie Arg Val Leu Leu Ala Asp Asp 1 5 Leu Arg Ala Val Leu u Ser Ala G u Val Ser Thr Ala G u G y Ala Val G n 40 Asp Val lie Leu IVet Asp Leu Arg Phe G n Val Ser Thr Gy Ala Asp Ala Thr 70 Asp Asn Pro Pro Lys Val Leu Val Val Asp Ile Leu G y Ala lle G u Ala G y 100 105 Asp Ala Pro Pro Ser G u Leu Leu Ala Hi s u I 10 Asp Ile Al a Al a G y Pr o Al a Al a Thr Asn 90 Al a Leu Ala Val Page 365 Val Val G u Val Lys Asp Tyr Ser Leu Gy Gy Gy Asn Asp Leu Al a seqI i st car r ect ed. t xt 115 120 125 G31y As p Se r Thr Leu Ser Pr o I'bt Val Ala Asn Arg Leu I'bt Thr Ar g 130 135 140 Val Ar g Thr Pr o Lys Thr Ser Leu Thr Pr o Ar g G31u Leu G31u Val Leu 145 150 155 160 Lys Leu Val Ala 31y O31y Ser Ser As n Ar g As p I Ie O31y Ar g I Ie Leu 165 170 175 Phe Leu Ser (31u Ala Thr Val Lys Ser Hi s Leu Val Hi s I Ie Ty r As p 180 185 190 Lys Leu G31y Val Ar g Ser Ar g Thr Ser Ala Val Ala Ala Ala Ar g (31u 195 200 205 G3 n G3 y Leu Leu 210 <210O> 299 <21 1> 655 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (632) <223> RXA01189 <400> 299 cacct gcaga aaaggaagct t aa at g at t t cc at t t cc at c gcc gac gac gaa 53 I'bt Ilie Ser Ilie Ser ie AlaAsp Asp Ou gcc A a tt a Le u t gg Tr p ct c Le u ac c Thr c aa O31n ac a Thr gga (31y at c I Ie gt c ValI g at As p ggC G31y g at As p aaa Lys5 ac c Thr cgc Ar g 125 ag c Ser cct Pr o ag c Ser g ac As p gcc A a gcg A a g at As p at c I Ie t cc Ser acc Thr aac As n ggC G31y gt g ValI 80 ct t Le u gaa G31u gac As p ct g Le u gca A a cga Ar g at c I Ie ct c Le u gca A a tt c Ph e CCC Pr o gca A a gga G31y ac c Thr 50 g ac As p at c I Ie gca A a gcc A a g aa G31u 130 ac c Thr t cc Ser g at As p ac c Thr gt g ValI ggt G31y ac c Thr 115 ct a Le u tt g Le u 20 ggt G31y gt a ValI gcc A a ac c Thr gt t ValI 1 00 gca A a gcc A a ct c Le u g aa G31u t gc Cys ac c Thr ag c Ser 85 tt a Le u at c I Ie gcc A a tt g Le u ct c Le u ct t Le u ct c Le u gcc A a tt c Ph e ac c Thr ac g Thr 135 g aa G31u at t lIe g ac As p at g vb t ag g Ar g tt g Le u gt t ValI 120 at c I Ie ccc Pr o g aa G31u ct t Le u g aa G31u ccc Pr o ccc Pr a 1 C ac Hi s ag c Ser g at As p ac g Thr C aa O31n ac c Thr cga Ar g aaa gct A a gcc A a Page 366 ggt O31y ggC G31y 1 55 ccg Pr o gcg A a tt g Le u seqI i st car r ect ed. t xt gaa t cc cca t ta acc aac cgt gaa gaa gaa gt c ct c O31u Ser Pro Leu Thr Asn Ar g O31u O31u O31u Val Leu 140 145 150 caa gga cta agc gcc gaa gaa att gcg gtg gca gcg (31n (1y Leu Ser Aia (1u (1u IIe Aa Val Aia Aia 160 165 gga acc acc cgc aac t at t ta t cc caa gct at g aca G31y Thr Thr Ar g Asn Tyr Leu Ser O31n Ala I'b t Thr 175 180 cag aat cgc ttt gaa gcg ttc acg cgc gcc agg gaa O31n Asn Ar g Phe G3 u A a Phe Thr Ar g A a Ar g G3 u 190 195 t agct tgt gg ct tat ctcct at t g aa O31u c ac Hi s aaa Ly s tt g Le u 200 ct a gca Leu A a ct c gcg Leu A a 1 gt a ggc Val G31y 1 ggc t gg G31y Tr p <210O> <211 <21 2> <21 3> 300 203 PmT Cor ynebact er i urn gi ut anri cumn <400> 300 I'bt I Ie Ser Ilie Ser Ilie Ala Asp Asp Le u A a Ar g I Ie Le u A a Ph e Pr o As n 145 O31u Tyr A a Thr Ser As p Thr ValI O31y Thr 115 Le u O31u I Ie Ser Thr 1 Le u O31u cys Thr Ser Le u I Ie A a O31u ValI 1 65 A a A a Ser O31u ValI Ar g 70 Hi s O31y Ar g vb t ValI 150 A a vb t Ar g Le u Le u Le u Le u A a Ph e Thr Thr 135 Le u A a Thr O31u Pr o O31u Le u O31u Pr o Pr a 1 05 Hi s Ser Le u Le u ValI 1 85 G31y O31u 10 As p Thr O31n Thr Ar g 90 Ly s A a A a A a A a 1 70 G31y Tr p A a Le u Tr p Le u Thr O31n Thr O31y O31y O31y 1 Pr a A a Le u Le u As p A a O31y Pr a Le u Ser Ar g O31u 140 O31n O31y O31n I Ie ValI As p O31y As p Ly s Thr Ar g 125 Ser O31y Thr As n A a Ar g Pr a I Ie Le u Ar g A a 110 Tyr Pr a Le u Thr Ar g 1 Ser Pr a Ser As p A a A a As p I Ie Le u Se r Ar g 1 Ph e <210O> 301 Page 367 seql i st cor r ect ed. t xt <211> 753 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(730) <223> RXA01607 <400> 301 gggctgaagg gctgggcgga acaataatta ttgaatctac tttccgcccg ttttccctat ccacaaaagg accaagataa aatcggatcg ggaactggaa atc cgt att li e Arg li e ttg Leu gt g Val gat Asp gat Asp gag G u gt g Val gct Al a at t SIle cag G n ct c Leu 150 agc Ser aaa Lys gat Asp gaa G u gtt Val cgt Ar g acg Thr acc Thr gcc Al a ggt Sy 120 gcc Al a gcc Al a aga Ar g cac Hi s cat Hi s gat Asp cgc Ar g ggt Gy cgc Ar g t ac Tyr ggg Gy cgc Ar g aag Lys gaa G u at c SIle 170 ggg Gy ccc Pr o ttt Phe gcc Al a gat Asp at c SIle 75 t cc Ser t at Tyr gat Asp at c SIle at t SIle 155 ggc Gy cat Hi s gtt Val gag G u gcg Al a caa G n cgt Ar g aca Thr ttg Leu gcc Al a at g Met 140 gaa G u aag Lys gt g Val gtt Val at a l Ie gaa Gu 45 cca Pr o gcg Al a gac Asp ct c Leu gcg Al a 125 ggg Gy gt g Val aaa Lys ttc Phe cgc Ar g gt g Val ggc Gy ggc Gy ct g Leu ggc Gy aaa Lys 110 cgg Ar g cgg Ar g ct g Leu ctt Leu aac Asn 190 gca Al a 15 gac Asp ggg Gy at c SIe gac Asp gat Asp 95 gat Asp gga Gy at g Met t cc Ser ttc Phe 175 aag Lys cgc Ar g 1 ggc ctt gcc G y Leu Al a atg gtg ggc Mat Val G y gtg gat gtg Val Asp Val gag gtc gcc Su Val Ala aac ccg cca Asn Pro Pro 80 gtg gtg ggc Val Val G y agc tcc cca Ser Ser Pro gaa t ca gt g G u Ser Val 130 aac aac ccc Asn Asn Pro 145 ttg gtg gcg Leu Val Al a 160 ct c act gag Leu Thr G u ctg gat gtc Leu Asp Val gga att at c S y lie lle 210 t cc Ser acc Thr gt g Val ggc Gy cag G n gca Al a gaa G u 115 ctt Leu at g Met caa G n gcc Al a acc Thr 195 ttg Leu cca Pr o ttg Leu ggg Sy gt a Val gt a Val 100 gat Asp t ca Ser act Thr ggg G y acg Thr 180 tct Ser ctg Leu ctg Leu gat Asp at g Met gta Val ctg Leu tct Ser ct c Leu aag Lys gct Al a caa G n 165 gtg Val aga Ar g aca gct gcg Thr Al a Al a 200 gta gct gaa gcc Val Ala u Ala aga cag Arg G n 205 tagacgcaca Page 368 seql i st cor r ect ed. t xt cgtgt t ggta acc <210> 302 <211> 210 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 302 Val Ile A 1 Leu Al a S Val Gy T Asp Val V Val Ala G Pro Pro G Val Gy A Ser Pro G 1 Ser Val L 130 Asn Pro IV 145 Val Ala G Thr Gu A rg Ile er Leu hr Pro al Leu ly Gy In Val I a Val 100 Iu Asp 15 eu Ser tt Thr In Gy I a Thr 1i Rf Leu 5 Leu Asp let Val Leu Ser Leu Lys Al a G n 165 Val Leu Al a Asp Asp Ser Al a Leu 55 Al a Val Gy Al a Val 135 Ser Asn Ser G u Val 40 Ar g Thr Thr Al a Gy 120 Al a Al a Ar g Hi s Asp Al a Phe Ar g Asn Val 105 Val Ser Ar g G u Met 185 Pr o Phe Al a Asp SIle Ser Tyr Asp SIle SIle 155 Gy Hi s Val G u Al a G n Ar g Thr Leu Al a Met 140 G u Lys Val Val SIle G u Pr o Al a Asp Leu Al a 125 Gy Val Lys Phe Al a Asp Gy I e Asp Asp Asp Gy et Ser Phe 175 Lys Asp Val Thr Ser Arg Thr Ala Ala Val Ala u Ala Arg n Arg Gy 195 200 205 le Ile 210 <210> 303 <211> 1392 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(1369) <223> RXN00470 <400> 303 tcataaccag gt t gggcaaa agggatgaat ccctggt t gt ggtggggct ct gaaaagta ctcatagact ctattgtgga gtgttgaggc tgataagtga atg ggg gaa agc cct 115 Iet G3y G u Ser Pro 1 Page 369 seql i st cor r ect ed. t xt gaa G u cat Hi s cag G n aga Ar g ctt Leu ttg Leu acg Thr tgg Tr p tcg Ser ggc Gy 150 ggg Gy ttg Leu gac Asp gt g Val ggc G y 230 gct Al a aag Lys gt g Val at g Met gcg Al a ggt Sy gt g Val agc Ser at t SIle tcg Ser 120 at g Met gt c Val gaa G u ct g Leu ttc Phe 200 gcg Al a gag Su cgc Ar g gcc Al a ttc agg gtc ttc cct Phe Arg Val Phe Pro gaa gat atg agt aac G u Asp IVet Ser Asn caa gcg gtg cct tta G n Al a Val Pro Leu 45 gtt gtt gcg ggt gtc Val Val Ala y Val gtg ttt tgg gtt cgt Val Phe Trp Val Arg 75 gcg ggt ctt ttt gcg Al a G y Leu Phe Al a aaa aag ggg agt ggg Lys Lys G y Ser G y 110 tgc ctg gtg ctg ctc Cys Leu Val Leu Leu 125 agc acc ggc ttc gcg Ser Thr G y Phe Al a 140 gtg ggc ctg ttg atg Val G y Leu Leu IVet 155 ggc ccg aat ctg ctg Sy Pro Asn Leu Leu 170 gcg atc gtg ctg atc Ala lie Val Leu Ile 190 atg gcg ctg gtg gcc IVet Ala Leu Val Ala 205 ggc gtt ccg ctg tgg Sy Val Pro Leu Trp 220 gcg gaa aaa gcc gca Al a u Lys Al a Al a 235 cat gat tcg gta ctg His Asp Ser Val Leu 250 gac ccc gcc gaa gtc Asp Pro Ala G u Val 270 gat ggt ttg gtg tcg Asp G y Leu Val Ser 15 aca cct gcg cct tat Thr Pro Ala Pro Tyr tat ccc acg ttt acc Tyr Pro Thr Phe Thr gca tcg ggg ctg gca Al a Ser G y Leu Al a gcg ctg ctg att ttt Ala Leu Leu Ile Phe 80 tat gcc ttg att tgg Tyr Ala Leu lie Trp 95 gag gcg tcg aca agc G u Ala Ser Thr Ser 115 gct atc ggt ggt gct Ala lle G y G y Al a 130 gtg ggc acg ttg gtg Val G y Thr Leu Val 145 gtg tgg ctg gcg tat Val Trp Leu Ala Tyr 160 att att gcc acc ggc Ile i e Al a Thr G y 175 gtg atg aat tgg aac Val IVet Asn Trp Asn 195 gtg gtg ctc acg ct g Val Val Leu Thr Leu 210 gtg cgg atg tgg gat Val Arg IVet Trp Asp 225 gct gct gag cgc gca Ala Ala G u Arg Ala 240 cag acc ttg gcg ctg G n Thr Leu Ala Leu 255 gcc cgc ctg gcc cgc Ala Arg Leu Ala Arg 275 Page 370 cag G n acc Thr cgg Ar g aag Lys gcg Al a at t SIle 100 aag Lys gcg Al a ccc Pr o gac Asp ggt Gy 180 acc Thr gt g Val cag G n gat Asp at t SIle 260 ggg Gy ggg G y ccg Pr o t ca Ser cat Hi s gcg Al a ttt Phe cgc Ar g gcg Al a at c SIe cgc Ar g 165 gtg Val cag G n ggt G y ctg Leu att SIe 245 caa G n cag G n seql i st cor r ect ed. t xt gaa cgc gag ctg cgt G u Arg G u Leu Arg 280 caa aca acc ggc act G n Thr Thr G y Thr 295 gtc gag gat att tac Val G u Asp Ile Tyr 310 gat gaa gcg ctg act Asp G u Ala Leu Thr 330 gaa gca ctc gtg aac G u Ala Leu Val Asn 345 gtg tac gcc gaa att Val Tyr Ala G u lie 360 cgc ggt gca gga ttc Arg G y Ala G y Phe 375 ctc gcc gaa tcc gt c Leu Ala G u Ser Val 390 cgc atc aaa tct gaa Arg lie Lys Ser u 410 caa tgg ctg ttt G n Trp Leu Phe 285 gtc ttt act gcg Val Phe Thr Al a 300 gct ctg cgt atc Ala Leu Arg Ile 315 gag aaa acg cag G u Lys Thr G r gtg gcc aag cat Val Ala Lys His 35C atg ctc ggc gas IVet Leu y G L 365 gac ccc gac aac Asp Pro Asp Asr 380 caa ggc cgc gtc Sn G y Arg Val 395 atc ggc gaa ggc lie y u G y gat Asp ttg Leu gt g Val gca Al a 335 gcc Al a ct g Leu at c SIle gaa G u acc Thr 415 t cc caa gat Ser G n Asp 290 gag cgc gcc G u Ar g Al a 305 cct gt g acc Pro Val Thr 320 gcg gtg at g Al a Val Mbet ggc gtg gaa G y Val G u aac att ttc Asn Ile Phe 370 ccc gac ggg Pro Asp G y 385 cga gcc ggc Ar g Al a G y 400 gaa gtg gca Su Val Ala aaa aca Lys Thr t gc ggt Cys G y gtg gga Val G y gca gt c Al a Val 340 acc gcc Thr Al a 355 gtc cgc Val Arg cac cac Hi s Hi s gga aaa G y Lys atc acc lIe Thr 420 979 1027 1075 1123 1171 1219 1267 1315 1363 1392 gat gtg Asp Val tagttggtcg tacgcgcgtg tct <210> 304 <211> 423 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 304 Vet y Gu Ser Pro u Lys Val Ala 1 5 Leu Val Ser n G y His Asp IVet lie 25 Ala Pro Tyr Thr Pro n Pro Ala y 40 Thr Phe Thr Arg Ser Arg Asp y Arg 55 G y Leu Ala Lys His Leu Asn Val Ser 70 Leu I e Phe Al a Al a Leu Leu Ser G y Leu Ile Trp Ile Phe Thr Arg Ile G u 100 105 Phe Arg Val 10 Su Asp IVet G n Al a Val Val Val Al a Val Phe Trp 75 Al a G y Leu 90 Lys Lys G y Page 371 Phe Ser Pr o Sy Val Phe Ser Pr o Asn Leu Val Ar g Al a Gy 110 Asp Thr Tyr Al a Al a Tyr G u Sy Pr o Pr o Ser Leu Al a Al a seqI i st car r ect ed. t xt Ser Thr Ser Lys Ar g Tr p 115 Val Ser 120 Tr p Cys Leu Val Leu Leu Al a Il e 125 G31y Th r 145 Le u Al a As n Le u vb t 225 G u Le u Le u O31n Ar g 305 ValI ValI ValI I Ie As p 385 Al a ValI Al a ValI Ty r G31y As n 1 95 Le u As p Al a Le u Ar g 275 Ly s Cys ValI Al a Th r 355 ValI Hi s G31y I Ie Al a I Ie Ar g 1 65 ValI O31n O31y Le u I Ie 245 O31n O31n Pr o O31u Th r 325 Ar g As p As p O31y ValI 405 vb t Ser O31y 150 O31y Le u As p ValI O31y 230 Al a Ly s O31u O31n ValI 310 As p O31u ValI Ar g Le u 390 Ar g As p ValI 135 ValI ValI vb t G31y Al a 215 O31u Ser Ar g Ar g Th r 295 O31u O31u Al a Ty r G31y 375 Al a I Ie ValI vb t ValI O31u Le u Ph e 200 Al a O31u Ar g Al a O31u 280 Th r As p Al a Le u Al a 360 Al a O31u Ly s Le u G31y Ser ValI 1 85 ValI Le u Ar g Le u As p 265 Le u Gy le Le u ValI 345 O31u G31y Ser Ser Ser ValI G31y 1 70 Al a vb t G31y Al a Hi s 250 As p Ar g Th r Ty r Th r 330 As n I Ie Ph e ValI O31u 410 Th r G31y 1 Pr o I Ie Al a ValI O31u 235 As p Pr o O31n ValI Al a 315 O31u ValI vb t As p O31n 395 li1e Ph e Le u Le u Le u ValI 205 Le u Al a ValI O31u Le u 285 Th r Ar g Th r Ly s O31y 365 As p Ar g O31u Al a vb t Le u I Ie 1 Al a Tr p Al a Le u ValI 270 Ph e Al a I Ie O31n Hi s 350 O31u As n ValI O31y ValI ValI I Ie 1 ValI ValI ValI Al a G n 255 Al a As p Le u ValI Al a 335 Al a Le u I Ie 0Ou Th r 415 <210O> 305 <211 <212> <213> <220> 1323 DNA Cor ynebact er i urn gi ut ani cumn Page 372 seql i st car r ect ed. t xt <221 OIDS <222> (1300) <223> FRXA00470 <400> 305 t ct at t gt gg agt gt t gagg ct gat aagt g aat gggggaa cagggt ct t c cct gat ggt t t ggt gt cgca ggggcat gac agccct gaaa aggt ggcgt t agt Ser cct Pr o ggt G31y gt t ValI tt t Ph e agt Ser ct g Le u tt c Ph e tt g Le u ct g Le u 1 50 ct g Le u gt g ValI ct g Le u gcc A a ac a Thr t at Tyr gca A a gcg A a t at Tyr gag (31u gct A a gt g ValI 120 gt g ValI at t I Ie gt g ValI gt g ValI gt g ValI 200 gct A a gcg A a ac g Thr ggg G31y ct g Le u tt g Le u t cg Ser ggt G31y ac g Thr ct g Le u gcc A a aat As n 1 70 ct c Le u at g vb t gag (31u t at Tyr ac c Thr gca A a tt t Ph e t gg Tr p ag c Ser gct A a gt g ValI t at Tyr 140 ggc G31y aac As n ct g Le u g at As p gca A a 220 ac c Thr cgg Ar g aag Lys5 45 gcg A a at t I Ie aag Lys5 gcg A a ccc Pr o 125 g ac As p ggt G31y ac c Thr gt g ValI c ag O31n 205 g at As p ccg Pr o t ca Ser cat Hi s gcg A a tt t Ph e cgc Ar g gcg A a 110 at c I Ie cgc Ar g gt g ValI c ag O31n ggt G31y 1 90 ct g Le u at t I Ie c ag O31n 15 ag a Ar g ct t Le u tt g Le u ac g Thr t gg Tr p 95 t cg Ser ggc G31y ggg G31y tt g Le u g ac As p 1 75 gt g ValI ggc G31y gct A a cct Pr o g at As p aat As n ct g Le u cgc Ar g 80 gt g ValI gt g ValI gt g ValI gt g ValI at g vb t 1 60 ggc G31y gct A a gag O31u t cc Ser at g vb t 1 gcg A a ggt G31y gt g ValI ag c Ser at t I Ie t cg Ser at g vb t gt c ValI g aa O31u 145 ct g Le u tt c Ph e gcg A a gag O31u cgc Ar g 225 at c gaa gat at g I I e 03 u Asp I'b t ggg caa gcg gt g O3 y 03 n A a Val cgg gt t gt t gcg Ar g Val Val Ala t cg gt g t tt t gg Ser Val Phe Tr p ggt gcg ggt ct t O3 y A a 03 y Leu gag aaa aag ggg O3 u Lys Lys (3 y t gg t gc ct g gt g Trp Cys Leu Val 1 00 ct g agc acc ggc Leu Ser Thr 03 y 115 ggt gt g ggc ct g G31y Val G31y Leu 130 t cc ggc ccg aat Ser G31y Pr o Asn gt g gcg at c gt g Val Ala Ilie Val 1 gt c at g gcg ct g Val I'b t A a Leu 1 ct g ggc gt t ccg Leu G31y Val Pr o 1 cgc gcg gaa aaa Ar g Ala O31u Lys 210 ct g cat gat t cg Leu Hi s Asp Ser gt a ct g cag acc t tg gcg ct g at t caa aag cgt gcc gac gac ccc gcc Page 373 seqI i st car r ect ed. t xt Val Leu O31n Thr Leu Al a Leu 1 1 230 235 e G3 n Lys g aa O31u ct g Le u act Thr cgt Ar g ac g Thr 310 aag Ly s ggc G31y g ac As p cgc Ar g gcc Al a g at As p tt g Le u 280 gt g ValI gca Al a gcc Al a ct g Le u at c I Ie 360 g aa O31u ct g Le u 250 c aa O31n cgc Ar g gt g ValI gt g ValI gt g ValI 330 at t I Ie g ac As p gcc Al a gcc Al a g at As p gcc Al a acc Thr at g vb t 315 gaa O31u t t c Ph e ggg O31y ggc O31y cgc Ar g aaa Ly s t gc cys gt g ValI 300 gca Al a ac c Thr gt c ValI c ac Hi s gga O31y 380 ggg O31y ac a Thr ggt O31y 285 gga O31y gt c ValI gcc Al a cgc Ar g c ac Hi s 365 aaa Ly s c ag O31n cct Pr o 270 g aa O31u ac c Thr cgc Ar g g at As p g ac As p 350 ggg G31y gt a ValI g aa O31u 255 c aa O31n gt c ValI g at As p g aa O31u gt g ValI 335 cgc Ar g ct c Le u cgc Ar g Ar g 240 cgc Ar g ac a Thr gag O31u g aa O31u gca Al a 320 t ac Tyr ggt O31y gcc Al a at c I Ie gag O31u ac c Thr g at As p gcg Al a 305 ct c Le u gcc Al a gca Al a g aa O31u aaa Ly s 385 ct g Le u ggc O31y at t I Ie 290 ct g Le u gt g ValI g aa O31u gga O31y t cc Ser 370 t ct Ser Al a As p cgt Ar g act Thr 275 t ac Tyr act Thr aac As n at t I Ie tt c Ph e 355 gt c ValI g aa O31u c aa O31n 260 gt c ValI gct Al a gag O31u gt g ValI at g vb t 340 g ac As p c aa O31n at c I Ie Asp Pro Al a 883 931 979 1 027 1 075 1123 1171 1219 1267 gaa ggc acc gaa gtg gca Ou Oy Thr Ou Val Ala 390 395 t ct <210> 306 <211> 400 <212> PRT <213> Cor ynebact er i urn gi <400> 306 I'b t I Ie O31u Asp I'bt Ser 1 5 Al a 31y O31n Al a ValI Pr o O31y Ar g Val Val Al a O31y Val Ser Val Phe Tr p Val Ser 03 y Al a 03 y Leu Phe 70 Il e 03 u Lys Lys 03 y Ser at c acc at g gat gt g I I e Thr I'bt Asp Val 400 t agt tggt cg t acgcgcgt g 1320 1323 ut ani cumn Asn Thr Pr o Leu Tyr Pr o 25 Val Al a Ser Ar g Al a Leu 55 Al a Ty r Al a 03 y 03 u Al a Al a Pro Tyr 10 Thr Phe Thr O3 y Leu Al a Leu Il e Phe Leu I Ie Tr p Ser Thr Ser Page 374 Pr o Ser Hi s Al a Ph e Ar g O31n Ar g Le u Le u Th r Tr p seqi i st car r ect ed. t xt Val Leu Leu Al a G3 y G3 y Al a Al a Ser ValI O31u 145 Le u Ph e Al a O31u Ar g 225 Al a O31u Thr As p Al a 305 Le u Al a Al a O31u Ly s 385 cys Ser 115 ValI G31y Al a vb t G31y 1 95 Al a Hi s As p Ar g Thr 275 Tyr Thr As n I Ie Ph e 355 ValI O31u (31y Le u As n ValI 1 65 Le u Pr o Ly s Ser Al a 245 Tr p Ph e Le u Ly s Al a 325 Le u Pr o G31y G31y Al a vb t 135 Le u I Ie Al a Tr p Al a 215 Le u ValI Ph e Al a I Ie 295 O31n Hi s O31u As n ValI 375 G31y ValI 120 ValI I Ie ValI ValI ValI 200 Al a O31n Al a As p Le u 280 ValI Al a Al a Le u I Ie 360 O31u Thr I Ie 1 05 (31y Tr p I Ie Nibt ValI 1 85 Ar g Al a Thr Ar g Ser 265 O31u Pr o Al a G31y As n 345 Pr o Ar g O31u Thr Le u Al a As n 1 70 Le u vb t O31u Le u Le u 250 O31n Ar g ValI ValI ValI 330 li1e As p Al a ValI Le u Al a Thr 1 55 Tr p Thr Tr p Ar g Al a 235 Al a As p Al a Thr vb t 315 O31u Ph e G31y G31y Al a 395 ValI Tyr 140 G31y As n Le u As p Al a 220 Le u Ar g Ly s cys ValI 300 Al a Thr ValI Hi s G31y 380 li1e Pr o 125 As p G31y Thr ValI O31n 205 As p O31y Thr O31y 285 O31y ValI Al a Ar g Hi s 365 Ly s Thr Al a 110 I Ie Ar g ValI O31n O31y 1 Le u I Ie O31n O31n Pr a 270 O31u Thr Ar g As p As p 350 O31y ValI vb t Ser (31y O31y Le u As p 1 ValI O31y Al a Ly s O31u 255 O31n ValI As p O31u ValI 335 Ar g Le u Ar g As p ValI ValI ValI vb t 1 O31y Al a O31u Ser Ar g 240 Ar g Thr O31u O31u Al a 320 Tyr O31y Al a I Ie ValI 400 <210O> 307 <211> 1119 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn Page 375 seqi i st car r ect ed. t xt <220> <221 OIDS <222> (1096) <223> RXA00756 <400> 307 at tcgct aaa cct gcgcagg at gagact gc cct cgcagaa cgcgt aaaca gt acgt ggt g gaagct tgag aggaagacaa agcacattcg acgaagccac c ag O31n tt g Le u cgc Ar g t cc Ser gga G31y gt c ValI gca A a gt t ValI at c I Ie tt g Le u 1 50 cgc Ar g c ag O31n g aa O31u at c I Ie tt a Le u cgc Ar g act Thr act Thr ct c Le u gcc A a gca A a t cc Ser 120 at c I Ie cca Pr o aag Ly s aac As n g aa O31u 200 cgc Ar g gcc A a at c I Ie gcg A a ac g Thr t cc Ser gct A a cga Ar g at c I Ie c ag O31n gag (31u tt c Ph e 1 70 t ct Ser cca Pr o gcc A a at c I Ie gcc A a t ct Ser gt c ValI gaa (31u 75 gt t ValI gca A a gt t ValI ct g Le u cag O31n 155 gt c ValI cgt Ar g gaa O31u gga (31y gag O31u t ct Ser gcg A a aac As n t ac Tyr cgc Ar g tt c Ph e c aa O31n ggt O31y 140 at c I Ie gt t ValI act Thr gt t ValI c ac Hi s 220 t ct Ser gca A a aac As n 45 gt c ValI g ac As p g ac As p g at As p gca A a 125 ac c Thr cct Pr o O31y c ac Hi s gct A a 205 cgc Ar g g aa O31u ct t Le u ct g Le u at c I Ie g ac As p gcg A a gcg A a 110 g at As p g aa O31u O31y gt g ValI cct Pr o 1 90 g ac As p t cc Ser aaa Ly s 15 tt g Le u aaa Ly s gt c ValI ac c Thr at c I Ie 95 t ac Tyr gcc A a gcg A a g aa O31u 99c O31y 1 75 gag O31u gga O31y aag Ly s gga O31y cat Hi s cca Pr a t ca Ser cca Pr a gt t ValI gca A a cgc Ar g g ac As p aag Ly s 1 aag Ly s ct g Le u t cc Ser gt t ValI gt g ValI 1 at c I Ie t cg Ser cgc Ar g g aa G u t cc Ser aag Ly s g at As p gca A a aac As n 145 ct c Le u ggc O3y gt g ValI gt g ValI gct A a 225 cca Pr a t ac Tyr gt g ValI tt c Ph e aac As n t cc Ser t at Tyr gct A a 130 c aa O31n aag Ly s aac As n cgc Ar g g aa O31u 210 gt t ValI gt t ValI at g vb t g ac As p g ac As p tt c Ph e ct g Le u g aa O31u 115 g aa O31u g ac As p c ac Hi s act Thr cga Ar g 1 at t I Ie c aa O31n cca Pr a g at As p at c I Ie g aa G u gga O3y cgc Ar g 1 00 ggc O3y cgc Ar g ggc O3y ggc O3y g ac As p 1 ct g Le u gt t ValI gcc A a aat at t gat Asn I I e Asp Page 376 gt g Val 230 cgt Ar g at c SIle cca Pr o gcg Al a gaa G u 310 gac Asp aac Asn t cc Ser t ac Tyr aag Lys 280 gt a Val caa G n cac Hi s aac Asn at c SIle 250 gaa G u gt c Val gt c Val gcc Al a gac Asp 330 gcc Al a 235 at g NMbt gat Asp aac Asn cct Pr o cgc Ar g 315 at c SIle aag Lys cgt Ar g cca Pr o gt a Val gac Asp 300 ttg Leu gat Asp seql i st cor r ect ed. t xt ggc gct tgc att ggc cca cgt Sy Ala Cys lie y Pro Arg 240 gaa ctc ggt gga gaa aaa atc G u Leu G y G y G u Lys Ile 255 gca acc ttc gtt gga aat gca Ala Thr Phe Val y Asn Al a 270 275 gag gtc acc gat ctt gaa gct G u Val Thr Asp Leu G u Ala 285 290 tac cag ctt tca cta gca atc Tyr G n Leu Ser Leu Ala lie 305 gct gcc cgc ctg acc ggc tgg Ala Ala Arg Leu Thr y Trp 320 taaaagtcgc ttgaaccggc atg gga Gy gac Asp 260 ct g Leu caa G n ggt Gy aag Lys cag G n 245 at c SIe gca Al a acc Thr aaa Lys at c SIe 325 835 883 931 979 1027 1075 1119 <210> 308 <211> 332 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 308 Val Asn Ile Asp Val n Ala Leu Lys Ala lie 1 5 10 I I e Pro Val Pro Asp Leu Leu Arg Thr lie Ala 25 Ser Tyr IVet Asp Asn Arg G u Thr Val Ala Ser 40 Arg Val Asp Ile Asp Ser Thr Thr y Thr Val 55 G u Phe Asp G u Asn G y G u Leu Al a Ser G u 70 75 Ser Asn Phe y Arg Val Ser Ala Arg Ala Val 90 Lys Ser Leu Arg G u Al a u Ala Ser Arg Ala 100 105 Asp Tyr G u y Thr Val Val Ser y lie Val 115 120 Al a Al a u Arg y Ile Ile Ile Val n Leu 130 135 Asn G n Asp G y Val Leu Leu Pro Ala G u G n 145 150 155 Leu Lys His G y Asp Arg Val Lys Cys Phe Val 165 170 G u Ser Al a Asn Tyr Ar g Phe G n Sy 140 I le Ser Al a Asn Val Asp Asp Asp Al a 125 Thr Pr o G u Leu Leu SIle Asp Al a Al a 110 Asp G u Sy Lys G y Leu Hi s Lys Pro Val Ser Thr Pro l e Val Tyr Al a Al a Ar g Al a Asp G u Lys 160 G y Lys 175 Val G y Val Page 377 Gy Val Val Al a 225 Gy G u Gy Leu Leu 305 Thr Thr Ar g 195 I le G n Ar g SIle Al a 275 Al a SIle Tr p SIle Phe Al a Lys G n 245 I le Al a Thr Lys SIle 325 G n Su l e Val 230 Ar g SIle Pr o Al a G u 310 Asp SIle Leu Ser 215 Lys Val Asp Ser Ar g 295 Gy lie seql i st cor r ect ed. t xt Asn Leu Ser Arg Thr His 185 Su Ile Pro u Val Ala 200 205 Arg G u Ala G y His Arg 220 Asn Leu Asn Al a Lys G y 235 Ser Asn lie IVet Arg u 250 Tyr Ser G u Asp Pro Ala 265 Lys Val Val Asn Val G u 280 285 Val Thr Val Pro Asp Tyr 300 G n Asn Ala Arg Leu Ala 315 His Ser Asp Ile Asp 330 Pr o 190 Asp Ser Al a Leu Thr 270 Val G n Al a G u Gy Lys Cys Gy 255 Phe Thr Leu Ar g <210> 309 <211> 834 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(811) <223> RXA00139 <400> 309 gaccggaaac gtactcaagg tagacacccg cgacggttcc ctaagattct taaaaccttt aagaatcagc cagaaacatt caa G n gga Gy gcg Al a aag Lys acc Thr gaa G u cgc Ar g t cc Ser gcg Al a acc Thr aac Asn aag Lys gat Asp gat Asp at c SIle gt g Val gcg Al a gtt Val acc Thr aat Asn 75 agc Ser cgc Ar g gat Asp aac Asn ggc Gy cgt Ar g cgt Ar g gt g Val at c SIle gcc Al a cga caa Arg G n 15 gcc gta Al a Val gcc at c Ala I e gtt gca Val Ala gtt gaa Val G u 80 Page 378 tacctctccc gcgttaacaa ttg att gaa cag gaa Leu lie G u G n G u 1 gat tac aag cga cac Asp Tyr Lys Arg His gac atc cta ttt gaa Asp Ile Leu Phe G u atc gat gac cgc cac Ile Asp Asp Arg His ccg gta gcg gaa tac Pro Val Ala G u Tyr ctc gat acc ctc gat Leu Asp Thr Leu Asp seql i gtc ttc ctc gcg gaa cac atc gca gaa Val Phe Leu Ala Gu His Ile Ala u cca tcc gtc gac cgc gca atc ctg cgc Pro Ser Val Asp Arg Ala lie Leu Arg 105 110 tac aac gcc gac gtt cct gtc acc acc Tyr Asn Ala Asp Val Pro Val Thr Thr 120 125 att gcc tcc gaa tac tcc gga gac aaa I e Al a Ser G u Tyr Ser G y Asp Lys 135 140 aca ctt gac gcc atg gca tca aag gtg Thr Leu Asp Al a IVet Al a Ser Lys Val 150 155 gcc aac cca gaa gca gtt ctg gcg gaa Ala Asn Pro G u Ala Val Leu Ala G u 170 gct ccg gtc gcg ccg tgg gat gac tcg Ala Pro Val Ala Pro Trp Asp Asp Ser 185 190 gaa gat ttt gag gct gta gat gct gct G u Asp Phe G u Ala Val Asp Ala Ala 200 205 act gta gag gtt tcc gaa gtc gca gaa Thr Val G u Val Ser G u Val Ala G u 215 220 tca gaa gaa aag gct gac gag agc taa< Ser G u G u Lys Ala Asp G u Ser 230 235 <210> 310 <211> 237 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 310 Leu lie u Gn u Gn Arg u n 1 5 Asp Tyr Lys Arg His G y Ser Arg Tyr 25 Asp Ile Leu Phe G u Al a u Ser Arg 40 Ile Asp Asp Arg His Lys Leu Ala Arg Pro Val Ala G u Tyr Thr G u Thr lie 70 Leu Asp Thr Leu Asp Val Phe Leu Ala Thr Leu y Arg Leu Pro Ser Val Asp 100 105 st cor r ect ed. t xt acc tgg act ct c Thr Trp Thr Leu gt c Val gca Al a t cc Ser gag G u gct Al a 175 gat Asp gag G u gac Asp gct Al a at c l Ie agt Ser acc Thr 160 t cc Ser gct Al a gtt Val tct Ser t cc Ser gtt Val gcc Al a 145 ct c Leu gaa G u ttg Leu ttt Phe gaa G u 225 tgg Tr p gaa G u 130 t ac Tyr cgc Ar g tct Ser gat Asp gag G u 210 gtt Val gga cga G y Arg 100 gaa at g G u Mt 115 gcc gtg Al a Val atc aac I e Asn gag cgc G u Arg ct c gat Leu Asp 180 gac tcg Asp Ser 195 gct gaa Al a G u tca aag Ser Lys ct c Leu at c SIe gaa G u gcg Al a gcc Al a 165 gat Asp gat Asp gag G u gtt Val atctttt ctggctaaca cca Asn Val 10 Lys Al a Asp Val Asp Thr I e Asn G u Hi s 90 Arg Ala I Page 379 G u et Pr o Pr o Val Al a Leu Ar g Ar g Val SIle Al a G u Ar g 110 Ar g Al a Al a Val Val Thr Val seqi i st car r ec Ser Tr p G31u l'bt I Ie Tyr Asn Ala Asp Val Pro 115 120 Val G31u Ala Val G31u I Ie Ala Ser G31u Ty r Ser 130 135 Ala Ty r I Ie As n Ala Thr Leu As p Ala l'b t Ala 145 150 155 Leu Arg G31u Ar g Ala Ala Asn Pro G31u Ala Val 165 170 G31u Ser Leu As p As p Ala Pr o Val Ala Pr o Tr p 180 185 Leu As p As p Ser As p G3 u As p Phe G3 u A a Val 195 200 Phe G31u Ala 31u G31u Thr Val G31u Val Ser G31u 210 215 G31u Val Ser Lys Val Ser G31u G31u Lys Ala As p 225 230 235 <210> 311 <211> 1458 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221> CDS <222> (1435) <223> RXA01303 <400> 311 aacat gcggg cgcaggt cag agct gt tat c t tagt actt a t gacggaaag cct t tccgcg t aaccat gaa gaggcat cac ted. t xt Val Thr 125 31y As p 140 Ser Lys Leu A a As p As p As p A a 205 Val Ala 220 G3 u Ser Thr Ly s ValI G31u Ser 1 A a aGu A a Ser G31u A a 1 As p G31u As p t cacagccat agggcgggct gtg aca caa ctc aac 11! Val Thr G3 n Leu Asn ac c Thr t gg Tr p at g vb t ct a Le u ct c Le u at g vb t ggC a31y t cg Ser at t lIe aat As n t ct Ser ct t Le u gt t ValI at t I Ie tt c Ph e at t I Ie CCC Pr o ccg Pr o ct g Le u gca A a t gc Cy s gga a31y ggt a31y 75 at t I Ie c aa a31n t gg Tr p gt g ValI tt t Ph e tt g Le u ct t Le u ggg a31y cga Ar g t gg Tr p 45 g at As p gcc A a gga a31y t gg Tr p ac c Thr t at Tyr ct c Leu ggC a31y ac c Thr g at As p 15 ct g Le u tt g Le u t ca Ser gga a31y cgc Ar g 95 cca Pr o t gg Tr p gt t ValI gca A a tt a Le u aaa Ly s 1 g aa a31u at t t ct Ser ggt a31y at c I Ie tt g Le u g at As p ac c Thr gcc A a c ag a31n cga Ar g gt c ValI cct Pr o ac c Thr at c I Ie ct t Le u tt g Le u gga a31y g aa a31u tt c Ph e gct A a t at Tyr at t I Ie at t I Ie 1 00 t cc ggt ct a t tt t tg at c ccc at g t tt ggg t gg Ser a31y Leu Phe Leu I Ie Pro l'bt Phe a31y Tr p t tc ct g gct gt c caa Phe Leu Ala Val a31n Page 380 NO seql i st cor r ect ed. t xt 105 110 115 Sgat tca agc act ccc tac tgg tgg ctt ctc aca ctc gct gca ctc act 499 Asp Ser Ser Thr Pro Tyr Trp Trp Leu Leu Thr Leu Ala Ala Leu Thr ,C 120 125 130 4 ggc att ggt ggt ggc gtg ttc tct gga tat atg ccg tcc acg gga tac 547 (3 y Ile G y G y G y Val Phe Ser G y Tyr 'b t Pro Ser Thr G y Tyr 135 140 145 ttc ttc ccc aag gca aaa tcg ggc act gcg ctg ggc att cag gca ggt 595 Phe Phe Pro Lys Ala Lys Ser G y Thr Ala Leu G y Ile n Ala G y c' 150 155 160 165 00 atc ggc aac ctc ggc gtc tcg ata att cag ttc atg ggc cca tgg gtc 643 lIe y Asn Leu y Val Ser Ile Ile n Phe 'b t y Pro Trp Val 0 170 175 180 .C atg ggt ttc ggt ctg ctg ggc att ggt ttc ctc acc ccg cag cgc acc 691 (2 Lt G y Phe G y Leu Leu G y Ile G y Phe Leu Thr Pro n Arg Thr 185 190 195 att gaa ggc acc acg gtg ttt gtg cac aat gct gcg att gtg ttg gtc 739 le u G y Thr Thr Val Phe Val His Asn Ala Ala lIe Val Leu Val 200 205 210 ccg tgg act att ctc gcg gcc gtt tta tcc ttc ctg ttt ctt aaa gat 787 Pro Trp Thr Ile Leu Ala Ala Val Leu Ser Phe Leu Phe Leu Lys Asp 215 220 225 gtc cca gtc acc gca aat ttc cgg caa cag atc gat atc ttt ggc aac 835 Val Pro Val Thr Ala Asn Phe Arg n On I le Asp Ile Phe y Asn 230 235 240 245 aag aac aca tgg att ttg tcc att atc tac ttg atg aca ttc ggt gcc 883 Lys Asn Thr Trp Ile Leu Ser Ile Ile Tyr Leu 'b t Thr Phe y Ala 250 255 260 ttc gcc ggt ttc gcc gcg cag ttc ggt ctg atc atc aac aac aac ttc 931 Phe Al a 0 y Phe Al a Al a 0 n Phe 0 y Leu Ile Ile Asn Asn Asn Phe 265 270 275 ggc atc gct tcc ccg atg gca gag act tat cca gct gag atg ctt cac 979 O y Ile Ala Ser Pro I'bt Ala 3 u Thr Tyr Pro Ala 3 u 'b t Leu His 280 285 290 gcc ggt gct acg ttc gcg ttt ctt gga cct ttg att ggt gct ttg gtg 1027 Ala y Ala Thr Phe Ala Phe Leu y Pro Leu Ile y Ala Leu Val 295 300 305 cgt gct gca tgg ggt cca ctg tgt gac aga ttc ggt gga gct atc tgg 1075 Arg Ala Ala Trp y Pro Leu Cys Asp Arg Phe y y Ala le Trp 310 315 320 325 acc ttt gtc ggt ggc atc gga atg act atc gcc act gca gct gcc gca 1123 Thr Phe Val y y Ile y 'b t Thr Ile Ala Thr Ala Ala Ala Ala 330 335 340 atc ttc cta agc aga gcg gag aca cct gat gat ttc tgg cca ttc ctg 1171 Ile Phe Leu Ser Arg Ala u Thr Pro Asp Asp Phe Trp Pro Phe Leu 345 350 355 tgg tcc atg ctt gcc ctg ttc ttc ttc acc ggt ctg ggc aat gct ggc 1219 Trp Ser 'b t Leu Ala Leu Phe Phe Phe Thr y Leu y Asn Ala y 360 365 370 acc ttc aaa caa atg ccc atg att ttg cct aaa cgc caa gca ggt ggc 1267 Thr Phe Lys n 'b t Pro 'b t Ile Leu Pro Lys Arg n Ala y y Page 381 gt g Val 390 ggt Gy gt g Val cgc Ar g ggc Gy ttg Leu ttc Phe aac Asn 440 acc Thr t cc Ser 410 at c SIle cca Pr o 380 gcc Al a act Thr gcc Al a ccg Pr o seql i st cor r ect ed. t xt 385 att ggt gcc ttc ggc ccc ttc att gtc lie y Ala Phe y Pro Phe lie Val 400 405 cca act gtc gcg ttc ttc tgg ggc tgc Pro Thr Val Ala Phe Phe Trp Gy Cys 415 420 acc gct ttg acc tgg atc tac tac gcc Thr Ala Leu Thr Trp lie Tyr Tyr Ala 430 435 gga t aaaccgaaa ggccaat cca t ga Gy 445 1315 1363 1411 1458 <210> 312 <211> 445 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 312 Val Thr G 1 Su Asp P Ile Thr T Ser Al a I Sy G n L li e Arg L Leu Val G Phe Leu A 1 Leu Al a A 130 Pro Ser T 145 G y lie Vbt Gy P Thr Pro G 1 Al a I e V 210 In Leu ro u hr Phe le Al a eu Tyr eu Il e ly Ile 100 I a Val 15 I a Leu hr G y In Al a ro Trp 180 In Arg 95 al Leu Asn 5 Hi s Ser Pr o Tr p Tyr Ser G n Thr Tyr Gy 165 Val Thr Val Thr Lys Trp Asp Ibet lie Leu Leu 55 Leu Al a 70 Mbt Phe Ser G y Asp Ser G y lie 135 Phe Phe 150 I e G y Vbt y Ile G u Pro Tr p 215 Val Lys Gy Ar g l Ie Pr o Phe 105 Thr Gy Lys Leu Gy 185 Thr SIle Val 10 SIle Phe SIle Pr o Pr o 90 Leu Pr o Gy Al a Gy 170 Leu Thr Leu Leu Al a Cys Gy Gy I le SIle Tyr Val Lys 155 Val Leu Val Al a G n Tr p Val Phe Leu Leu Pr o Tr p Phe 140 Ser Ser Gy Phe Al a 220 Gy Ar g Tr p Asp Al a Gy Met Tr p 125 Ser Gy SIle SIle Val 205 Val Asp Leu Leu Ser Gy Ar g Gy Leu Tyr Al a G n 175 Phe Asn Ser Leu Phe Leu Lys Asp Val Pro Val Thr Ala Asn Phe Arg G n n lie Page 382 seql i st cor r ect ed. t xt 235 225 Asp Met SIle Al a SIle 305 Gy Thr Phe Leu Ar g 385 Gy Phe Tr p lie Phe G y Thr Phe G y 260 Asn Asn Asn 275 G u let Leu 290 G y Al a Leu G y Al a I e Al a Al a Al a 340 Trp Pro Phe 355 G y Asn Al a 370 G n Al a G y Pro Phe Il e Phe Trp G y 420 II e Tyr Tyr 435 Asn 245 Al a Phe Hi s Val Tr p 325 Al a Leu Sy Sy Val 405 Cys Al a Lys Asn Thr Al a SIle Sy 295 Al a Phe Phe Ser Phe 375 I le Val Val Pr o Gy Al a 280 Al a Al a Val Leu let 360 Lys Sy Leu Phe Asn 440 Trp Ile 250 Phe Al a 265 Ser Pro Thr Phe Trp y G y G y 330 Ser Arg 345 Leu Al a G n let Trp Thr Leu Ser 410 Phe Il e 425 Ala Pro Leu Ser Ile Ile Tyr Leu 255 Al a n Phe G y Leu I e 270 IVet Ala G u Thr Tyr Pro 285 Ala Phe Leu y Pro Leu 300 Pro Leu Cys Asp Arg Phe 315 320 I e y IVet Thr Ile Ala 335 Ala G u Thr Pro Asp Asp 350 Leu Phe Phe Phe Thr G y 365 Pro IVet Ile Leu Pro Lys 380 G y Al a I e G y Al a Phe 395 400 Phe Thr Pro Thr Val Ala 415 i e Ala Thr Ala Leu Thr 430 Phe Pro G y 445 <210> 313 <211> 327 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(304) <223> RXA01412 <400> 313 cgccttcttt gcagctgaga attaactcac ccttgccctt ataccccata ggggtatagc cttgagggag agagtacttc acctgaaagg ggccagtgac atg gca tta aag aac IVet Ala Leu Lys Asn 1 tac acc gtt gag ggc atg acc tgc gca cac tgc gtg gca tcg gta act Tyr Thr Val G u G y Mbt Thr Cys Ala His Cys Val Ala Ser Val Thr 15 gaa gag gta agc gaa gtt aat ggc gtt agc gct gtt gac gtc act cta G u G u Val Ser G u Val Asn y Val Ser Al a Val Asp Val Thr Leu 30 gaa tca gga aac gtc gct gtc agt ggc gaa ggt ttc agc gat gca gag Page 383 seqI i st car r ect ed. t xt (31u Ser G31y Asn Val Al a Val Ser G31y 31u G31y Phe Ser Asp Al a (31u 45 at c cag gct gct gta gag gaa gcc ggc t ac aag at c gt t gcc t cc I Ie O31n Al a Ala Val Ou Ou Ala Gy Tyr Lys lieVal Ala Ser 60 t aaagcaccc aagaacat tt aaa <210> 314 <211> 68 <212> PRT <213> Cor ynebact er i urn gI ut anri curn <400> 314 I'b t Al a Leu Lys Asn Tyr Thr Val O31u G31y I'bt Thr Cys Al a Hi s Cys 1 5 10 Val Al a Ser Val Thr O31u O31u ValI Ser O31u Val Asn G31y Val Ser Al a 25 Val Asp Val Thr Leu O31u Ser G31y Asn Val Al a ValI Se r G31y O31u 31y 40 Phe Ser Asp Al a Gu IIe Gn Al aAl aVal G u GuAl a GyTyr Lys 55 Ilie Val Ala Ser <210> 315 <211> 1266 <212> DNA <213> Cor ynebact er i urn gI ut ani curn <220> <221> OIDS <222> (1243) <223> RXA00773 <400> 315 gcccccaaaa agt gaaagca caccact t tc ct agt tgcgc cct gct caca at t tgct t aat att t tgc ccaacct gat t cacggggga caat agt tag gt g act t ta aaa at c a ggc G31y gt a ValI cgc Ar g cgt Ar g gcg Al a tt t Ph e aac As n gga G31y ct t Le u g at As p ct t Le u gct Al a at c I Ie gag (31u aat As n gcc Al a t t c Ph e t cg Ser cgc Ar g ccc Pr o 75 t cc Ser cgc Ar g ggg G31y aat As n cga Ar g gt g ValI ct g Le u t ac Tyr aaa Lys5 tt g Le u Val Thr Leu Lys I I e 1 gt t ct a gcc ccc at g gct ggt Val Leu Al a Pr o I'b t Al a G31y 15 t gc cgt gaa cag gaa at g caa Cys Ar g 31u O31n 31u [vb t O31n gt c t gt gaa at g gt g act gcg Val Cys O31u I'b t Val Thr Al a acc at g cac at g acc acc tt c Thr I'b t Hi s I'b t Thr Thr Phe cag ct g t ac acg gt t gac ccg (31n Leu Tyr Thr Val Asp Pr o 80 Page 384 seql i st cor r ect ed. t xt aag tac acc tac Lys Tyr Thr Tyr gat Asp cgg Ar g at c SIle acg Thr 150 gat Asp cac Hi s gag G u ttg Leu gag G u 230 agg Ar g at c SIle cac Hi s gat Asp ggc G y 310 gag G u gcg Al a at t SIle ggc G y 120 t cc Ser aag Lys gga G y cgc Ar g gcg Al a 200 aat Asn act Thr tgg Tr p gag Su gaa G u 280 cgt Ar g ttc Phe ct c Leu ggt Sy gaa gcg gcg aag atg atc gtt Su Ala Ala Lys IVet lie Val 95 atg aac ttt ggc tgc ccg gtt Met Asn Phe Gy Cys Pro Val 110 tct gcg att cct tac aag cgc Ser Ala lie Pro Tyr Lys Arg 125 gct gtg aag gct acg gaa ggc Ala Val Lys Ala Thr G u y 140 cgc gtt ggt att gat gat gag Arg Val Gy Ile Asp Asp u 155 160 att gct gtc gac gcc ggc gcg lie Ala Val Asp Ala Gy Ala 170 175 gcg gcg cag cgc tat tcc ggt Ala Al a G n Arg Tyr Ser y 190 ctg aag gag cat ttg gca gat Leu Lys G u His Leu Ala Asp 205 gat att ttc gcg gca tcc gat Asp Ile Phe Ala Ala Ser Asp 220 tgc gat ggc gtc gtg gtt ggg Cys Asp y Val Val Val y 235 240 ttt gct gag ctg tct gct gct Phe Al a G u Leu Ser Ala Ala 250 255 cct acc ttc ggc gaa gtt acc Pro Thr Phe Gy G u Val Thr 270 ctc atg cag cat gat ggc gaa Leu IVet n His Asp Gy u 285 cac atg ggt tgg tac ctg cgc His Iet G y Trp Tyr Leu Arg 300 tcc aat ctg gcc aag gtt tcc Ser Asn Leu Ala Lys Val Ser 315 320 gca cca tgg gct gac tcc acc Ala Pro Trp Ala Asp Ser Thr 330 335 cga ggt cga cag ggc gct cct Arg G y Arg n G y Ala Pr o 350 gat gaa aac Asp G u Asn cca aag gt c Pro Lys Val 115 cgt ttg ttt Arg Leu Phe 130 acg gac att Thr Asp Ile 145 cac cat act Hi s Hi s Thr aag t cc gt a Lys Ser Val gag gct gat G u Al a Asp 195 acc ggc at c Thr G y Ile 210 gca acg cgc Al a Thr Ar g 225 cgt ggt tgc Arg y Cys gtt cgt gga Val Arg y caa at c at c G n lie lle 275 acc aag ggg Thr Lys G y 290 ggt ttc cct G y Phe Pr o 305 acc tat gt g Thr Tyr Val gcc aag gca Al a Lys Al a gca aag gt g Al a Lys Val 355 ttg Leu 100 acg Thr gaa G u ccg Pr o cac Hi s gcg Al a 180 tgg Tr p cca Pr o at g Met ct g Leu gaa G u 260 ct g Leu ct g Leu gtt Val gag G u gag G u 340 gca Al a gcg Al a cgc Ar g aac Asn gt g Val ttg Leu 165 ctt Leu aac Asn gtt Val at g Met ggc Gy 245 gaa G u cgc Ar g cgc Ar g ggc G y ctt Leu 325 gac Asp ctt Leu 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 Page 385 seqI i st car r ect ed. t xt cca gat ggc t gg t tg gac gat cct gag gat gcc act gt t cct aaa ggc Pr o Asp G31y Tr p Leu Asp As p Pro Ou Asp Ala Thr Val Pro Lys Gy 360 365 370 gca gaa at g gaa aac tcc gga ggg t agt taat tt aat act tacc ccc Ala O31u 'b t O31u As n Se r 31y G31y 375 380 <210> 316 <211> 381 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn 1219 1266 <400> 316 Val Thr Leu Lys Ilie Gy Pro Phe Asp A a (31u G u Hi s Tyr As p Pr o Ar g Thr 145 Hi s Ly s (31u Thr A a 225 Ar g ValI vb t (31u ValI Thr ValI As n ValI 115 Ph e I Ie Thr ValI As p 1 95 li1e Ar g cys G31y G31y O31n A a Ph e Pr o A a Ar g As n ValI Le u 1 65 Le u As n ValI vb t G31y 245 (31u Thr Thr A a Pr o Tyr Hi s G31y ValI 135 ValI A a A a I Ie G31y 215 O31n Pr a Pr o As n G31y 40 Le u As p Thr I Ie G31y 120 Ser Ly s G31y Ar g A a 200 As n Thr Tr p O31u ValI Thr ValI O31u Tyr As p 1 G31y A a Ph e Ar g Thr 1 Ar g G31y G31y Le u O31u 265 Le u 10 A a I Ie G u As n 0Ou 90 vb t Ser A a Ar g I Ie 1 70 A a Le u As p cys Ph e 250 Pr a A a Ph e Ser Ar g Pr a A a As n A a ValI ValI 1 A a A a Ly s I Ie As p 235 A a Thr Pr a Thr Le u G u Ser Ly s 0Gy Pr a 125 A a I Ie As p Ar g Hi s 205 A a ValI Le u 0Gy ValI cys ValI Thr O31n lIe Pr a Ly s (31u As p G31y 1 Ser A a Ser ValI A a 255 ValI On Ilie Ilie Leu Arg His Al a 0u Leu Leu I'bt O n Hi s Asp 0 y 0 u Page 386 seql i st cor r ect ed. t xt 275 280 285 Thr Lys Gy Leu Arg Asp Leu Arg Lys His Iet G y Trp Tyr Leu Arg 290 295 300 G y Phe Pro Val G y G y G u Phe Arg Ser Asn Leu Ala Lys Val Ser 305 310 315 320 Thr Tyr Val G u Leu G u Asp Leu Leu Ala Pro Trp Ala Asp Ser Thr 325 330 335 Al a Lys Al a G u Asp Ala G u G y Ala Arg G y Arg Gn G y Ala Pro 340 345 350 Ala Lys Val Ala Leu Pro Asp y Trp Leu Asp Asp Pro G u Asp Ala 355 360 365 Thr Val Pro Lys y Al a G u Iet u Asn Ser G y y 370 375 380 <210> 317 <211> 290 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> <223> RXA02746 <400> 317 ggt gtg cag ggc atg act gtc acc gaa acc caa ggc ttt ggc cag cag Sy Val n y Mbt Thr Val Thr u Thr n y Phe y n n 1 5 10 aaa ggc cac acc gag gtg tac cgt ggt gct gaa tac gct gtc gat ttt Lys G y His Thr G u Val Tyr Arg G y Ala G u Tyr Ala Val Asp Phe 25 gtg cct aag gtc aag att gaa gtt att atc tcc gat gct cag gct gag Val Pro Lys Val Lys Ile u Val Ile Ile Ser Asp Ala n Ala u 40 gaa gtc atc aac att atc gtc gag acc gca cgc acc ggc aaa gtc ggc Su Val lie Asn Ile Ile Val u Thr Ala Arg Thr y Lys Val y 55 gac ggc aaa gtg tgg atg act aac atc gaa gag ctg gtt cgt gtt cgt Asp y Lys Val Trp IVet Thr Asn lie G u G u Leu Val Arg Val Arg 70 75 acc ggt gag cgc ggc gaa gca gcc ctt taaaaactta tgaataatcc Thr y u Arg y u Ala Ala Leu agc <210> 318 <211> 89 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 318 Gy Val Gn Gy Mbt Thr Val Thr Gu Thr Gn y Phe Gy Gn n 1 5 10 Page 387 Lys G y Hi s Thr Val Pro Lys Val Su Val Ile Asn Asp G y Lys Val Thr Gy G u Arg G u Lys SIle Tr p Gy seql i st cor r ect ed. t xt Val Tyr Arg Gy Ala G u Tyr Ala Val Asp Phe 25 Ile Gu Val Ile Ile Ser Asp Ala Gn Ala Gu 40 I le Val G u Thr Ala Arg Thr Gy Lys Val Gy 55 IVet Thr Asn Ile G u G u Leu Val Arg Val Arg 70 75 G u Ala Ala Leu <210> 319 <211> 902 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> <223> RXA02745 <400> 319 gcc gga at c Al a G y li e 1 gga cct aat G y Pro Asn ttt acc gcc Phe Thr Al a gcc aca gaa Al a Thr G u ggt ggc ggt G y G y G y gcg gcg tcc Al a Al a Ser ctt gta gga Leu Val G y ggt ggc caa Sy y n 115 cct gag at t Pro u Ile 130 t ca cgc aac Ser Arg Asn 145 ttc gaa gtc ccc cgc ggt Phe G u Val Pro Arg y 5 10 gca ggc aaa acc acc acc Al a G y Lys Thr Thr Thr acc tct ggc agc atc cga Thr Ser G y Ser Ile Arg 40 gac cag gtg cgc cga cgc Asp G n Val Arg Arg Arg 55 tac agc gga atc cgc gtg Tyr Ser y lie Arg Val 70 aac gac aac cca cac gat Asn Asp Asn Pro His Asp 90 cgt gaa caa cgc aaa acc Arg G u G n Arg Lys Thr 105 caa cgc ctt tct ttg gcc G n Arg Leu Ser Leu Ala 120 ttc ctc gac gaa ccc acc Phe Leu Asp G u Pro Thr 135 gtg tgg gag ctt gtc aac Val Trp G u Leu Val Asn 150 caa gtt ttg gcc ctc ctg G n Val Leu Ala Leu Leu at t SIle gt c Val at c SIle ttt Phe 75 cct Pr o acc Thr tta Leu gct Al a gat Asp 155 gaa G u ttg Leu ggc G y gaa G u gaa G u tac Tyr gca Al a ggc G y 140 ct c Leu at g Met ggc G y at c SIle at g Met tgg Tr p cga Ar g tta Leu 125 at g Met cgc Ar g gaa G u gat Asp ctt Leu aag Lys ctt Leu ct g Leu ggt G y gcg Al a gac Asp ggt G y cca Pr o caa G n ctt Leu gat Asp t ca Ser cgc Ar g caa G n ggc 160 gtc acc atc gtg ctc acc acc cac ctg atg gat gag gcc gaa gca cta Page 388 seql i st cor r ect ed. t xt Val Thr lie Val Leu Thr Thr His Leu Met Asp G u Ala G u Al a Leu 165 gct gac cac gtg atc atc Ala Asp His Val Ile Ile 180 aca cct gat gaa ctc act Thr Pro Asp G u Leu Thr 195 tcc gta gag acc acg agc Ser Val G u Thr Thr Ser 210 ctc agc agc tta aac atc Leu Ser Ser Leu Asn Ile 225 230 ctg cat tat tca ctt cgg Leu His Tyr Ser Leu Arg 245 cac atc gtc cag gct gt c His Ile Val n Ala Val 260 gat acg gga cac cgc tca Asp Thr y His Arg Ser 275 aaa gaa ctg agg agt taac Lys G u Leu Arg Ser 290 <210> 320 <211> 293 <212> PRT <213> Corynebacter i um gl <400> 320 Ala y Ile Ser Phe u 1 5 G y Pro Asn G y Ala G y Phe Thr Ala Pro Thr Ser Ala Thr G u Pro Asp G n G y G y G y Ser Tyr Ser 70 Ala Ala Ser Tyr Asn Asp Leu Val y Leu Arg G u 100 G y G y G n G n Gn Arg 115 Pro G u Ile lie Phe Leu 130 170 175 gtt Val gcg Al a ccg Pr o 215 ggt G y acg Thr gcc Al a ttg Leu gcc Al a caa G n 200 ctt Leu gat Asp caa G n cgc Ar g gaa G u 280 aac Asn 185 cgc Ar g gat Asp gt g Val caa G n caa G n 265 gat Asp ggt G y gat Asp ctt Leu aaa Lys gcc Al a 250 aac Asn gt c Val caa G n cat Hi s gat Asp gca Al a 235 acc Thr gt c Val ttc Phe ctt Leu gaa G u 205 ttg Leu gcc Al a gat Asp at t SIle gac Asp 285 gcc Al a 190 at t SIle gt g Val aac Asn t cc Ser cgc Ar g 270 at c SIle agt Ser aat Asn gat Asp cgg Ar g ttg Leu 255 tct Ser acc Thr gcacac cat gt ct aaa cct ut ari cum Val Pro Arg Lys Thr Thr 25 Sy Ser lie 40 Val Arg Arg 55 Sy lie Arg Asn Pro Hi s G n Arg Lys 105 Leu Ser Leu 120 Asp G u Pro 135 G y G n Val 10 Thr Ile G u Arg Val Leu Arg lie G y Val Phe G u Asp Pro u 90 Thr Thr Tyr Al a Leu Al a Thr Al a G y 140 Page 389 Leu bet Sy I le et Tr p Ar g Leu 125 M\et Al a Cys I le et Leu Leu Ar g 110 SIle Asp Leu G u Asp Leu Lys Leu Leu Sy Al a seql i st cor r ec Ser Arg Asn IVet Val Trp G u Leu Val Asn Asp 145 150 155 Val Thr I e Val Leu Thr Thr Hi s Leu bet Asp 165 170 Ala Asp His Val Ile I e Val Ala Asn G y n 180 185 Thr Pro Asp G u Leu Thr Ala G n Arg Asp His 195 200 Ser Val G u Thr Thr Ser Pro Leu Asp Leu Asp 210 215 Leu Ser Ser Leu Asn I e Gy Asp Val Lys Ala 225 230 235 Leu His Tyr Ser Leu Arg Thr n G n Ala Thr 245 250 His Ile Val n Ala Val Ala Arg n Asn Val 260 265 Asp Thr G y His Arg Ser Leu G u Asp Val Phe 275 280 Lys G u Leu Arg Ser 290 <210> 321 <211> 486 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(463) <223> RXN00820 <400> 321 acttccacca ccccaaacca tcgtttcttt cgaagacgca cctgggcttt tcgcagtggc gcactgtcac ccaggagat g ted. txt Leu Arg G u Al a I e Leu Leu G u 205 Arg Leu 220 Arg Ala Pro Asp Met Ile Leu Asp 285 Ar g G u Al a 190 I le Val Asn Ser Ar g 270 I le Asp Al a 175 Ser Asn Asp Ar g Leu 255 Ser Thr Sy 160 Leu Sy Val Asp Pr o 240 Al a Leu Gy gac Asp aag Lys t cc Ser acc Thr gt c Val act Thr ggt Sy at c SIle aag Lys gt c Val gat Asp ttt Phe ccg Pr o aac Asn t cc Ser cag G n ggc G y tgg Tr p ggc G y at c SI e tgg Tr p gca Al a ggc G y 45 ct g Leu cgc Ar g at t SIle at t I e gag G u gat Asp at g M\et cac act Hi s Thr 15 gcc cac Al a Hi s ctt ctc Leu Leu aag gt g Lys Val ggc gct G y Al a 80 Page 39C ccaaccctca ccggccagga gtg aac acc ttg gcg Val Asn Thr Leu Ala 1 gat cct gag cgc gcc Asp Pro G u Arg Ala ggt ttc ctc acc ttg G y Phe Leu Thr Leu gat gtc acc ggc gtg Asp Val Thr G y Val cgt ttc acc tct ccc Arg Phe Thr Ser Pro gtg gtc cgt gag atc Val Val Arg G u Ile ^0 seql i st cor r ect ed. t xt t ct gag gtg aag ggc aat ggc ctg cac ctg gtc gcc gat ggc act att 403 Ser G u Val Lys G y Asn G y Leu Hi s Leu Val Al a Asp G y Thr I e 95 100 S gag atc gaa ggg cag gag cgc ccg gcc gtc gta gct acc ttc ctc acc 451 L 3 u lie u y Gn u Arg Pro Ala Val Val Ala Thr Phe Leu Thr 105 110 115 cgc ttc tac gct taaaagcttg cttctcgacg caa 486 Arg Phe Tyr Ala 120 00 <210> 322 0 <211> 121 0 <212> PRT C1 <213> Cor ynebact eri um gl ut am cum NO 0 <400> 322 0 Val Asn Thr Leu Ala Asp Ala Thr Asp Asp n n Trp lie His Thr (C1 1 5 10 Asp Pro G u Arg Al a Lys Asp G y Pro Phe G y G y Ala lie Ala His 25 G y Phe Leu Thr Leu Ser IVet IIe Ile Pro Phe Trp G y G u Leu Leu 40 Asp Val Thr G y Val Thr Thr Lys Val Asn Tyr G y Leu Asp Lys Val 55 Arg Phe Thr Ser Pro Val Lys Val y Ser Arg Ile Arg IVet y Ala 70 75 Val Val Arg G u Ile Ser G u Val Lys G y Asn G y Leu His Leu Val 90 Ala Asp y Thr Ile u Ile u G y n G u Arg Pro Ala Val Val 100 105 110 Ala Thr Phe Leu Thr Arg Phe Tyr Ala 115 120 <210> 323 <211> 486 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(463) <223> FRXA00820 <400> 323 acttccacca ccccaaacca tcgtttcttt cgaagacgca ccaaccctca ccggccagga cctgggcttt tcgcagtggc gcactgtcac ccaggagatg gtg aac acc ttc gcg 115 Val Asn Thr Phe Ala 1 gac gca act gat gat cag cag tgg att cac act gat cct gag cgc gcc 163 Asp Ala Thr Asp Asp On On Trp lie His Thr Asp Pro G u Arg Ala 15 aag gac ggt cct ttt ggt ggc gca att gcc cac ggt ttc ctc acc ttg 211 Lys Asp G y Pro Phe y y Ala lie Ala His G y Phe Leu Thr Leu Page 391 t cc Ser acc Thr gt c Val tct Ser gag G u cgc Ar g at c I e aag Lys gt c Val gt g Val gaa G u t ac Tyr 120 seql i st cor r ect ed. t xt ccg ttc tgg ggc gag ctt ctc gat gtc Pro Phe Trp Gy G u Leu Leu Asp Val 45 aac tat ggc ctg gat aag gtg cgt ttc Asn Tyr y Leu Asp Lys Val Arg Phe tcc cgc atc cgc atg ggc gct gtg gtc Ser Arg I e Arg IVet Gy Ala Val Val 75 ggc aat ggc ctg cac ctg gtc gcc gat G y Asn G y Leu Hi s Leu Val Al a Asp 95 cag gag cgc ccg gcc gtc gta gct acc Sn G u Arg Pro Ala Val Val Ala Thr 110 taaaagcttg cttctcgacg caa ggc Gy tct Ser gag G u act Thr 100 ct c Leu <210> 324 <211> 121 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 324 Val Asn Thr Phe Ala Asp Ala Thr Asp 1 5 Asp Pro G u Arg Ala Lys Asp y Pro Sy Phe Leu Thr Leu Ser IVet lie lle 40 Asp Val Thr G y Val Thr Thr Lys Val Arg Phe Thr Ser Pro Val Lys Val y 70 Val Val Arg G u Ile Ser G u Val Lys Ala Asp y Thr lie Gu lie Gu y 100 105 Ala Thr Phe Leu Thr Arg Phe Tyr Ala 115 120 <210> 325 <211> 732 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(709) <223> RXA01059 G n Gy Phe Tyr Ar g Asn G u Tr p Al a Gy Leu Ar g Leu Pr o Hi s Al a Leu Lys Gy Leu Val Page 392 seqi i st car r ect ed. t xt <400> 325 aat t at t agc t t t ccaat ac aaaat t t aaa t cccagagcg at ct gcccca cact t act t g at gcgggaac aaat t tgaag gt tt t tcagt t gct at aggt at g act aca gt t act l'bt Thr Thr Val Thr c aa O31n gag O31u c ag O31n aac As n gct A a gca A a g aa O31u g aa O31u ct t Le u gct A a 1 50 tt c Ph e cca Pr o g aa O31u ct t Le u cgc Ar g g aa O31u c ag O31n ct c Le u cct Pr o ccc Pr o g at As p 120 at c I Ie cca Pr o g ac As p g ac As p gt c ValI 200 gca A a gca A a at c I Ie ct g Le u cca Pr a gt c ValI ct c Le u gct A a t ac Tyr ac c Thr cgc Ar g 1 70 gct A a ct t Le u aat As n t t c Ph e cgc Ar g t t g Le u 75 gca A a t t c Ph e t ca Ser gcc A a ggc G31y 155 cac Hi s t gg Tr p g ac As p gct A a g ac As p gt g ValI at g vb t ct t Le u cca Pr a cgt Ar g at c I Ie 140 at g vb t cgc Ar g t ac Tyr g aa O31u tt c Ph e ct a Le u 45 gt a ValI gca A a ct t Le u cct Pr a gct A a 125 at c I Ie g at As p gt t ValI g ac As p g ac As p act Thr gt t ValI at t I Ie g aa O31u gca A a tt c Ph e 110 t cc Ser ggt G31y gca A a ct g Le u cgc Ar g 1 90 gca A a 15 g at As p aag Ly s gt t ValI ggc G31y gcc A a 95 cca Pr a t cc Ser at c I Ie g ac As p gt t ValI 1 75 ct g c ag O31n g aa O31u t gg Tr p cgt Ar g aac As n 80 g ac As p ggc O31y gca A a cgc Ar g gct A a 1 60 gcc A a cca 1 aac As n cca Pr a gca A a t cc Ser 65 c ag O31n at c I Ie gca A a g aa O31u gca A a 145 at c I Ie at c I Ie cgc ct c Le u at c I Ie cca Pr a 50 g aa O31u gcc A a g ac As p cgc Ar g ct c Le u 130 gca A a t cc Ser aac As n ct t ct t Le u t ct Ser 35 ac c Thr g aa O31u aag Ly s tt c Ph e g ac As p 115 aat As n ggt O31y aag Ly s at g vb t gag O31u 1 tt c Ph e 20 g ac As p gca A a gcc A a gt t ValI c ac Hi s 1 00 at g vb t gct A a ct c Le u gag O31u ggt O31y 1 c ag O31n cgt Ar g gag O31u at g vb t aaa Ly s gct A a g aa O31u tt c Ph e ggc O31y gcc A a tt c Ph e 1 aag Ly s g ac As p Leu Pro Arg Leu gaa acc ct c 03 u Thr Leu t agaaaccac t ct agaaat a gct <210O> 326 <21 1> 203 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 326 N/t Thr Thr Val Thr 31n Asp Leu Leu AiaLeu Asp (1u Asp Aia (1n 1 5 10 Page 393 seqi i st car r ect ed. t xt Asn Leu Leu Phe Arg G3 u Al a Ar g Th r Al aAsn Al a Phe Thr Asp G u Pr o Al a Ser O31n I Ie Al a O31u Al a 145 le le Ar g Ser Thr O31u Ly s Ph e As p 115 As n (31y Ly s vb t O31u 1 O31u vb t Ly s Al a O31u Ph e G31y Al a Ph e 1 65 Ly s As p O31n As n Al a 70 Al a O31u O31u Le u Al a 150 Ph e Pr o O31u I Ie Ser Ar g Al a vb t Al a O31n 135 G31y Pr o Al a ValI Al a I I e Phe Pro Leu Ar g Val Pr o Leu 75 Al a Val Al a 90 Lys Leu Phe 1 05 03 u Al a Ser 03 y Tyr Al a [vb t Thr G3 y 1 55 G31y Ar g Hi s 1 70 Asn Al a Tr p 1 85 03 u Thr Leu Le u ValI Al a Le u Pr o Al a 125 li1e As p ValI As p ValI I Ie (31u Al a Ph e 110 Ser G31y Al a Le u Ar g 1 Lys5 ValI G31y Al a Pr o Ser I Ie As p ValI 1 Le u Tr p Ar g As n As p G31y Al a Ar g Al a 1 Al a Pr a <210O> 327 <21 1> 1053 <21 2> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 ODS <222> (1030) <223> RXN01386 <400> 327 ctct at tgt g gt acgcacca t gact gct ca accagcccac gttcagtacg agcgggatcc tcccggacaa tcgcgtccaa gagccgccaa ggcatct ccg at g t gg gag ggc cac aat As n ccc Pr a gcg Al a at c cgc Ar g c ag O31n gt a ValI g aa tt g Le u t cc Ser ggg O31y ccc ct t Le u gaa O31u act Thr ac g ccg Pr a ac c Thr t cg Ser ggt ct c Le u aac As n c aa O31n 45 gt g g ac As p ct g Le u tt t Ph e gt t l'b t Trp Gu Oy His 1 at t aga acc at t gac gat cgc I Ie Ar g Thr I Ie As p As p Ar g 15 cac ct c cca t ca at g cgg at g Hi s Leu Pr o Ser l'b t Ar g l'b t gt c gag cgt t ca gag agt tt c Val O31u Ar g Ser O31u Ser Phe gcg at c t tc t tt gcg act gaa Page 394 seql i st cor r ect ed. t xt Val Val Ala lie Phe Phe Ile Ser u Asn Pro Thr ggt gaa Gy Gu ggt cag G y G n aac aat Asn Asn ctt gaa Leu G u ttc gga Phe G y 135 cag gaa Gn G u 150 t cc agt Ser Ser cac gga Hi s G y ggc tta G y Leu agt gac Ser Asp 215 cgc caa Arg G n 230 tac gt c Tyr Val ccg gag Pro G u ttc gct Phe Al a atg acg Met Thr 295 gca Al a gcc Al a cgc Ar g at t SIle 120 gca Al a tct Ser gga G y ctt Leu at t SIle 200 tta Leu ct a Leu ct a Leu cga Ar g t cc Ser 280 ccg Pr o ttc Phe gtt Val cgc Ar g ggc G y gga G y cac Hi s tta Leu 170 cgc Ar g gca Al a gcg Al a cga Ar g acc Thr 250 aag Lys agt Ser caa G n tt c Phe 75 tac Tyr gag G u t ca Ser acc Thr agg Ar g 155 gga G y aat Asn gcc Al a tcg Ser at c SIle 235 cga Ar g gtt Val cat Hi s tgg Tr p Sy cac Hi s gac Asp ct a Leu aaa Lys gcc Al a 140 at t SIle aaa Lys gt a Val cag G n cgg Ar g 220 ttc Phe ct g Leu tcg Ser ttc Phe agg Ar g 300 Al a Thr G u cgt Ar g gcc Al a gtt Val ggc G y 125 aat Asn gaa G u ccg Pr o ctt Leu aga Ar g 205 at t SIle t ca Ser gat Asp gt c Val agt Ser 285 aag Lys ggt G y gat Asp ct c Leu 110 cct Pr o gaa G u agt Ser tgg Tr p ggc G y 190 ttt Phe gct Al a gac Asp ttt Phe agt Ser 270 cgc Ar g gaa G u gga G y agg Ar g 95 acc Thr gag G u caa G n ggc G y agc Ser 175 gac Asp at t SIle gca Al a t ca Ser gca Al a 255 gag G u acc Thr tcg Ser cat Hi s 80 cca Pr o at c SIle ctt Leu gct Al a gag G u 160 gat Asp gcc Al a gac Asp gcc Al a gga G y 240 aag Lys at c SIle ttc Phe cag G n gt a Val ttc Phe ccg Pr o ccc Pr o tta Leu 145 ccg Pr o at c SIle aca Thr at c SIle gt g Val 225 caa G n gaa G u ggt G y cgc Ar g cgt Ar g 305 gcg Al a ct c Leu aag Lys gct Al a 130 gcg Al a aag Lys gag G u agt Ser aat Asn 210 gga G y act Thr gcg Al a aag Lys gag G u 290 caa G n ctt Leu cgc Ar g cag G n 115 at t l Ie cga Ar g cat Hi s cac Hi s agc Ser 195 at t SIle at c SIle at c SIle ct g Leu cgc Ar g 275 cgg Ar g t cc Ser cgg Ar g gga G y 100 cgc Ar g ttt Phe ct a Leu at c SIle gag G u 180 gaa G u t cc Ser agc Ser gga G y tcg Ser 260 ttt Phe ttt Phe ttt Phe cca Pr o ttc Phe t ac Tyr gag G u gtt Val gat Asp 165 gcc Al a gaa G u gaa G u gaa G u cgc Ar g 245 aca Thr ggg G y gaa G u caa G n 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1053 gag 31u 310 tgaggttttt gttctcaggc gga Page 395 ND 2seql i st cor r ect ed. t xt <210> 328 <211> 310 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 328 e IVbt Trp G u G y His Asn Ala Arg Ala Leu Leu Pro Leu Asp IIe Arg 1 5 10 Thr I e Asp Asp Arg Pro Iet G n Ala Ser G u Thr Asn Leu His Leu 25 C Pro Ser MIt Arg Mbet Ala Ser Val Phe y Thr Ser G n Phe Val G u 0 35 40 00 0 Arg Ser u Ser Phe lie Ser u Asn Pro Thr y Val Val Ala lle 55 \O Phe Phe Ala Thr G u G y G u Ala Val Phe Phe His Arg G y G y His S 65 70 75 Cq Val Ala Leu Arg Pro G y G n Ala lle Val Tyr Asp Ala Asp Arg Pro 90 Phe Leu Arg y Phe Asn Asn Arg Phe Arg G u Leu Val Leu Thr Ile 100 105 110 Pro Lys G n Arg Tyr Leu G u Ile Val y Ser Lys y Pro G u Leu 115 120 125 Pro Al a lie Phe G u Phe G y Al a Thr G y Thr Al a Asn G u n Al a 130 135 140 Leu Ala Arg Leu Val G n G u Ser Leu His Arg lie G u Ser G y G u 145 150 155 160 Pro Lys His lie Asp Ser Ser G y Pro Leu G y Lys Pro Trp Ser Asp 165 170 175 lie G u His G u Ala His G y Leu lie Arg Asn Val Leu G y Asp Ala 180 185 190 Thr Ser Ser u u y Leu Ile Ser Ala Ala n Arg Phe Ile Asp 195 200 205 lie Asn lie Ser u Ser Asp Leu n Ala Ser Arg Ile Ala Ala Ala 210 215 220 Val y Ile Ser G u Arg G n Leu Ser Arg lie Phe Ser Asp Ser y 225 230 235 240 G n Thr lie y Arg Tyr Val Leu Asn Thr Arg Leu Asp Phe Ala Lys 245 250 255 G u Ala Leu Ser Thr Pro G u Arg Asp Lys Val Ser Val Ser G u lie 260 265 270 G y Lys Arg Phe G y Phe Ala Ser Pro Ser His Phe Ser Arg Thr Phe 275 280 285 Arg G u Arg Phe G u IVet Thr Pro Leu G n Trp Arg Lys u Ser G n 290 295 300 Arg G n Ser Phe n G u 305 310 <210> 329 Page 396 seql i st cor r ect ed. t xt <211> 1806 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1783) <223> RXN00073 <400> 329 gaatctaatg gt t ggtctag acagagcggt acgtctaagt gtgacatgta cttcactagc tctttaagga ttaactcccc ttgcggatag atcaaaccga gga Gy caa G n aag Lys t ac Tyr cgc Ar g ggt Sy ttc Phe ct g Leu gac Asp gat Asp 150 at g let tct Ser gcg Al a gcc Al a aaa Lys gaa Gu aag Lys aag Lys ct g Leu at g Met gcc Al a 120 acc Thr cct Pr o ggt Sy gt a Val cgt Ar g 200 cca Pr o gac Asp ccc Pr o ggt Sy ttg Leu ggt Sy gt g Val ggt Sy cgc Ar g at c SIle ggt Sy 170 gct Al a cgc Ar g gca Al a ggc G y gct Al a ttt Phe ggc Gy 75 cag G n cgt Ar g gaa G u cag G n tgg Tr p 155 gac Asp gaa G u tac Tyr cgt Ar g acc Thr ttt Phe tct Ser at t SIle ctt Leu ttt Phe at t SIle aac Asn 140 gag G u gtt Val gag G u ct a Leu gcc Al a gag G u gct Al a 45 t cc Ser t ac Tyr cct Pr o gat Asp tct Ser 125 at t SIle aag Lys cca Pr o ct g Leu gac Asp 205 gcc Al a ccg Pr o gt c Val at t SIle acc Thr gat Asp ggc Gy 110 agg Ar g cag G n ct a Leu cgt Ar g at c SIle 190 aag Lys agg Ar g 15 ctt Leu aag Lys gca Al a cag G n gat Asp 95 gga Sy gat Asp ct g Leu gaa G u gtt Val 175 gat Asp gaa G u aag Lys aac Asn cag G n ccg Pr o cgt Ar g gag G u ct g Leu t at Tyr cac Hi s acc Thr 160 at c SIle gcc Al a gag G u atg aca lMt Thr 1 cct aag Pro Lys cat gcc Hi s Al a cgg gtc Arg Val gat gac Asp Asp aag cag Lys G n ctg cag Leu G n gct t cc Al a Ser gct cgt Al a Ar g 130 tgg att Trp Ile 145 gtc gga Val G y ttg ggc Leu G y acc ccg Thr Pro ttc cac Phe Hi s 210 aca acc acc Thr Thr Thr ccc Pr o gag G u at t SIle at t SIle gat Asp gat Asp cct Pr o 115 t cc Ser cgt Ar g ct g Leu t cc Ser gct Al a 195 aac Asn gaa G u gaa G u gat Asp gcc Al a ctg Leu gag G u 100 gag G u acc Thr att SIe t cc Ser cca Pr o 180 at c SIe ctt Leu Page 397 cgt aag Arg Lys 215 cac gaa Hi s G u 230 ggc cca G y Pro atg ctt lIet Leu gaa gt g G u Val cgc ctg Arg Leu 295 att gag Ile G u 310 ct g at c Leu II e att ggc I e G y cca acc Pro Thr gtt gca Val Ala 375 gaa ct g u Leu 390 cgc gac Arg Asp cgc ggc Arg G y gca acc Al a Thr cgc ct c Arg Leu 455 cct aac Pro Asn 470 ttt aag act gct atc ac Phe Lys Thr Ala lie Th 220 seql i st cor r ect t ggc aac cag r y Asn n ed. t cgc cag gat gtt acc G n Asp Val Thr at c SIle gga Gy gct Al a tgg Tr p 280 cgt Ar g aag Lys gat Asp at t SIle gtt Val 360 gaa G u ct c Leu ct g Leu at c SIle acc Thr 440 ggc Gy tct Ser gac Asp gag Gu 250 cca Pr o ggc Gy cgt Ar g cgt Ar g cca Pr o 330 cca Pr o cac Hi s cac Hi s ct c Leu gaa Gu 410 t cc Ser t ca Ser ttg Leu gca Al a gtt Val 235 tgc Cys ctt Leu gt c Val gct Al a gaa Gu 315 gtt Val caa G n acc Thr ggc Gy gat Asp 395 at c SIle tgc Cys cga Ar g gat Asp cgc Ar g 475 t cc Ser ttt Phe ggt Gy gcc Al a cgc Ar g 300 gtt Val gtt Val aag Lys acc Thr at c SIle 380 at t SIle gga Gy acc Thr gca Al a gtt Val 460 acc Thr gtt Val ggc Gy tgg Tr p 270 at t l Ie aag Lys gag Gu acc Thr ggc Gy 350 gag G u agg Ar g aga Ar g t ac Tyr ttg Leu 430 gag G u at c SIle gtt Val tcg Ser 240 ggc Gy cca Pr o cgc Ar g ttg Leu gaa Gu 320 cct Pr o ttc Phe ct c Leu cgt Ar g aac Asn 400 t ca Ser ttc Phe gt c Val at t SIle gac Asp 480 Ar g 225 at t SIle ct g Leu ctt Leu gac Asp gt g Val 305 t ac Tyr ggc Gy t ac Tyr at t SIle acc Thr 385 ctt Leu cct Pr o tgc Cys gac Asp gca Al a 465 at c SIle cac Hi s t cc Ser gat Asp t ac Tyr 290 gca Al a ct c Leu t ac Tyr ct c Leu gcc Al a 370 acg Thr act Thr t cc Ser aag Lys gaa G u 450 ct g Leu gga Gy cca Pr o acc Thr gag G u 275 ggc Gy cag G n gag Gu cgt Ar g ggt Gy 355 at t l Ie gcg Al a acc Thr gag G u ctt Leu 435 ct g Leu aac Asn ttc Phe gaa G u aac Asn 260 gtt Val ttc Phe tgg Tr p cgc Ar g gac Asp 340 gt g Val gct Al a gaa G u gtt Val ttc Phe 420 gcg Al a gaa G u ggt Gy aag Lys ttc Phe 245 cca Pr o cca Pr o cga Ar g ggt Gy aag Lys 325 cac Hi s aag Lys gat Asp aag Lys gca Al a 405 cgc Ar g cac Hi s gag G u tgc Cys gga 485 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 1363 1411 1459 1507 1555 Page 398 cag acc gtc act gat gct gac ggc a G n Thr Val Thr Asp Al a Asp Gy A 490 cac ctg ggc ggt tcc atg aac ttg g Hi s Leu G y G y Ser ebt Asn Leu A 505 5 aag ggc cac aag gtt att gcc gat g Lys G y His Lys Val Ile Ala Asp C 520 525 gtt gtt acc cac ttc aag gaa cag c Val Val Thr His Phe Lys G u G n A 535 540 gat tgg gtc cag cgg gcc gct gag g Asp Trp Val G n Arg Al a Ala u C 550 555 gaggaaaccc aat <210> 330 <211> 561 <212> PRT <213> Corynebacter i um gl ut ai cum 1 i st cor r ect ed. t xt iac cgc gtt gaa ggt ttc cag gtt sn Arg Val G u y Phe G n Val 495 500 at cca aac ttc gga cgc aag ctc ,sp Pro Asn Phe G y Arg Lys Leu ;10 515 iaa gtg gga gag tac gtc act cgc Su Val y G u Tyr Val Thr Arg 530 gc cac gag gac gag cac ttc cgc rg His G u Asp G u His Phe Arg 545 aa gat ttg gtg tgagtcttcg Su Asp Leu Val 560 1603 1651 1699 1747 1793 1806 <400> 330 Mat Thr Thr 1 Pr o Hi s Ar g Asp Lys Leu Al a Al a Tr p 145 Val Leu Pr o G u I le SIle Asp Asp Pr o 115 Ser Ar g Leu Ser Thr Thr G y Ser Ala Arg 5 Ou Gy On Trp Lys Ile 25 G u Ile Lys Gn u G u 40 Asp Ile Tyr Ser Lys G n Ala Pro Arg Phe Lys Trp 70 Leu G y G y G u Leu Thr G u Tyr Phe IVet IVet Arg 100 105 G u Arg Leu Arg Ala Val 120 Thr Ala Asp Phe Thr Asp 135 Ile G u Asp Val Pro Ala 150 Ser Thr IVet Leu G y Cys 165 Pro Val Ser y Val Ala 180 185 Pr o 10 Asp Pr o Sy Leu Sy 90 Val Sy Ar g l Ie Sy 170 Al a Al a Sy Al a Phe Sy G n Ar g G u G n Tr p 155 Asp G u Al a G u Al a Ser Tyr Pr o Asp Ser 125 I le Lys Pr o Leu Asp 205 Al a Pr o Val SIle Thr Asp Sy 110 Ar g G n Leu Ar g SIle 190 Ar g Leu Lys Al a G n Asp Sy Asp Leu G u Val 175 Asp Thr Pro Al a 195 I e Asp Ala li e G u Arg Tyr Leu Lys G u G u Page 399 s Phe His Asn Leu Pro Arg Lys Phe 210 215 eql i st cor r ect ed. t xt Lys Thr Ala lie Thr Sy Asn n Ar g 225 I le Leu Leu Asp Val 305 Tyr Gy Tyr SIle Thr 385 Leu Pr o Cys Asp Al a 465 I le G u Phe G u Asp 545 Val Asp Pr o Thr G u 275 Gy G n G u Ar g Gy 355 I le Al a Thr G u Leu 435 Leu Asn Phe Phe Ar g 515 Val Hi s Thr Phe 245 Pr o Pr o Ar g Gy Lys 325 Hi s Lys Asp Lys Al a 405 Ar g Hi s G u Cys Gy 485 Val Leu Ar g Ar g G u Pr o Leu Val Leu 295 G u SIle Gy Thr Al a 375 Leu Asp Gy Thr Leu 455 Asn Thr Leu Sy Val 535 Tr p G n Phe G n 265 Al a Asn Phe Sy Hi s 345 Sy Lys Phe Asp SIle 425 Lys Asp Cys Thr Sy 505 Lys Hi s G n Val 235 Cys Leu Val Al a G u 315 Val G n Thr Gy Asp 395 I le Cys Ar g Asp Ar g 475 Al a let SIle Lys Al a 555 220 Ser Phe Gy Al a Ar g 300 Val Val Lys Thr SIle 380 I le Gy Thr Al a Val 460 Thr Asp Asn Al a G u 540 Al a Phe Val Ser Gy 285 Leu Leu Thr Asp Gy 365 Thr G u Leu Gy l Ie 445 Pr o G n Sy Leu Asp 525 G n G u Pr o Sy 255 I e Phe Phe Thr Asn 335 Lys G n SIe Lys Ser 415 G u Leu Lys Ser Ar g 495 Pr o Val Hi s Asp Page 400 seql i st cor r ect ed. t xt <210> 331 <211> 373 <212> DNA <213> Corynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(373) <223> RXN03131 <400> 331 aacgcggctc gtgaagatta cctgaaaaac caagccctcc accagttggc taaaccaaaa cgtatttaag gagaaacacc cct Pr o cgc Ar g cca Pr o ggc G y gcc Al a gct Al a t cc Ser ggc Gy cag G n acc Thr cag G n acc Thr aaa Lys gca Al a cct Pr o at c SIle gaa G u ttc Phe gct Al a cct Pr o tgg Tr p gag G u gat Asp 75 acc Thr tta Leu gac Asp gag G u ctt Leu gaa G u gt g Val tgg Tr p ttt Phe cca Pr o ggc G y gct Al a 15 ggc Gy ct c Leu t ac Tyr aag Lys ggc Gy gt g Val t ac Tyr ggc G y cgc Ar g 80 agcgtccgct gctcggctaa atg acc act tct gta Met Thr Thr Ser Val 1 gaa aac cca ggc ctg Su Asn Pro y Leu tgg ttc cca gag gat Trp Phe Pro G u Asp gag gtc gtg aaa gct G u Val Val Lys Ala tac ctg cca acc gat Tyr Leu Pro Thr Asp ggc ctg aag ctg tcc G y Leu Lys Leu Ser <210> 332 <211> 91 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 332 bet Thr Thr Ser Val Pro Ala Ser Thr 1 5 Su Asn Pro y Leu Arg lie y Thr 25 Trp Phe Pro G u Asp Pro Lys G n Ile 40 G u Val Val Lys Ala G y Tyr Thr Trp 55 Tyr Leu Pro Thr Asp Al a Asn G n Leu 70 G y Leu Lys Leu Ser Al a G y Thr G u Lys Ala Leu Ser Val Ala y 10 Ala Pro Asp Ser Trp Gy Val Pro Trp G u Arg Phe Leu Tyr Ile u Leu y Pro Tyr y G u Asp G u Leu y Lys Arg 75 Phe Thr Page 401 seqI i st car r ect ed. t xt <210O> <211 <21 2> <21 3> <220> <221 <222> <223> 333 549 DNA Cor ynebact er i urn gi ut anm curn CIDS 101). (526) RXS001 53 <400> 333 cctcgatttg agtaaagagg acgtcctcgc ctgatttttc agccct gcct ggccct tcca aat tat gtag ggt ggcct gc t gg t tt at c gga gca t tg gt t ct t gct ggc tt g Tr p Phe I Ie G31y Al a Leu Val Leu Al a G31y Leu 15 gggt gt gt tt t tgcgt ggcg gt g ValI 1 gga gca ata G3 y Al a I Ie gaa t tg gca G3 u Leu Al a gt a ggt Va1(3 Gy gag t tc acc G3 u Phe Thr gt g gca ct c Val Al a Leu t tg at g ct c ggc Leu I'bt Leu G31y ggt G31y gca gct t tg gca Al aAl a Leu Al a acc gcc ggc Thr Al a (3 y acc ttc gcg Thr Phe Al a at c ggt gt c cca I Ie a31y ValI Pr o gt a ValI 45 t gg gct gaa tt t Tr p Ala a31u Ph e gt c ValI gt g gcc Val Ala t ca gct gct ct a Ser Al a Al a Leu at g t tc at t agg I'bt Phe I Ie Ar g gcc at t aga aag Ala IlieArg Lys cgt Ar g ct g ct g aaa cca Leu Leu Lys Pro gt t ct g gac t ct Val Leu Asp Ser t ca Ser cca cga gca ctt Pro Arg Ala Leu ggc cac cgt gct ay His Arg Ala g aa a31u gt g ct c gaa gat Val Leu a31u Asp gga gcg acc agc a3 y Al a Thr Ser ggg cag a31ya(1n 1 00 gt c cgc ct g Val Ar g Leu cac acc ttc Hi s Th r Phe 120 ggt t ca at t t gg a31y Ser I Ie Tr p gcc cgc agc at g Al a Ar g Se r I'b t gat ccc aca Asp Pro Thr 115 at c caa ggc I Ie a31n a31y gcg gaa ggt gaa Al a a3 u a3 y a3 u gt c agt gt c at t Val Ser Val I Ie g at As p 130 acg acc Thr Thr 135 gcg at t gt a t gg Ala IlieVal Trp aaa gaa gcc Lys a3 u Al a 140 t aaat t t t t a acaat caaat <210O> 334 <21 1> 142 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm curn <400> 334 Va a31yAia Ilie IlieTrp Phe Iiea3y 1 5 Al a Leu Val Leu Al a a31y Leu 10 (3u Leu Aa Val (3y (3u Phe Thr Leu Leu N/t Leu(3y (3y Ala Ala 25 Page 402 seql i st cor r ect ed. t xt Leu Ala Thr Ala G y Val Ala Leu IIe Gy Val Pro Val 40 Phe Val Thr Phe Ala Val Ala Ser Ala Ala Leu Leu MVet 55 Pro Ala lie Arg Lys Arg Leu Leu Lys Pro Lys Val Leu 70 Pro Arg Ala Leu Val G y His Arg Ala G u Val Leu G u 90 Ala Thr Ser Gy G n Val Arg Leu Asp y Ser I e Trp 100 105 Ser IVet Asp Pro Thr His Thr Phe Ala G u G y G u Ile 115 120 125 I e Asp lie n y Thr Thr Ala I e Val Trp Lys u 130 135 140 <210> 335 <211> 509 <212> DNA <213> Cor ynebact eri um gl ut am cum Tr p Phe Asp Asp Ser 110 Val Al a Al a SIe Ser Val Al a Ser <220> <221> CDS <222> <223> RXN01716 <400> 335 gaa G u 1 gtt Val gca Al a gaa G u cgc Ar g ct g Leu gag G u gac Asp ttc Phe act Thr ct g Leu gag G u cat Hi s gcg Al a aag Lys gct Al a ggt Gy 115 gtt Val gag G u 5 aaa Lys act Thr gt a Val aac Asn cgt Ar g ct g Leu ttc Phe acc Thr gga Gy ggc Gy cgt Ar g gag G u cgc Ar g 70 cct Pr o t cc Ser tgt Cys tac Ty r ttc Phe gt c Val gt c Val gcc Al a gat Asp gga Gy ttc Phe gtt Val gac Asp aaa Lys gac Asp gct Al a 40 gt a Val gaa G u t ac Tyr cga Ar g at c SIle 120 ggt Gy gag G u gcc Al a ttg Leu cga Ar g ttc Phe caa G n ggt Gy 105 gac Asp gaa G u at c I e 10 acc Thr gaa G u at g Met tt t ttt Phe gct Al a 90 gca Al a ctt Leu acc Thr ggt Gy aac Asn gtt Val tcg Ser 75 gaa G u at c l Ie ggc Gy cgt Ar g cag G n t ac Tyr gcc Al a at g Met gt a Val gtt Val ggt Gy gaa G u ct a Leu gtt Val acc Thr acc Thr at t SIle gac Asp gga Gy 125 acc Thr ccc Pr o ct c Leu gca Al a cag G n ggc Sy cct Pr o act Thr at c SIe gag G u at g Met acc Thr ctt Leu gaa G u gaa G u gag G u atc cta ggc tcc cac tca acc IIe Leu y Ser His Ser Thr Page 403 seqI i st car r ect ed. t xt 130 135 140 caa atg gga tgc gtg cgc ctg acc gaa cga atc atg cgcagcgac cca 480 O31n 'b t 31y Cy sValI Ar g Leu Thr OuArg Ile I'bt Arg Ser Asp Pro 145 150 155 160 ccc gac tgaaaccgaa gtggaaatcg ccc 509 Pr o Asp <210> 336 <211> 162 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani curn <400> 336 Ou Val Thr Pro Ou Gy Phe Lys Ou lieThr ValI A a (31u Ar g Le u O31u As p Ph e O31n 145 Pr o Le u O31u Hi s A a Ly s A a G31y 115 ValI G31y Ly s Thr ValI As n Ar g Le u Ph e Thr ValI ValI ValI A a As p G31y Ph e ValI As p 135 Le u As p A a 40 ValI O31u Tyr Ar g I Ie 120 G31y Thr A a Le u Ar g Ph e O31n G31y 1 As p (31u (31u 10 Thr (31u vb t Ph e A a 90 A a Le u I Ie Ar g G31y As n ValI Ser (31u I Ie G31y Le u I Ie 1 (31u Le u ValI Thr Thr I Ie As p G31y 125 Ser Ar g Thr Pr o Le u A a O31n O31y Pr o Thr Ser As p <210O> 337 <21 1> 534 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani curn <220> <221 CIDS <222> (511) <223> RXN02972 <400> 337 acct acgacg gt gaaat cct aggct cccac t caacccaaa t gggat gcgt gcgcct gacc gaacgaatca tgcgcagcga cccacccgac tgaaaccgaa gtg gaa atc gcc cgc 115 Val Ou Ilie Ala Arg 1 Page 404 IN seql i st cor r ect ed. t xt gac tac gtt gca gaa cgc atc cag gaa gta aaa gcc atc gtc cca att 163 s Asp Tyr Val Ala G u Arg Ile G n G u Val Lys Ala lie Val Pro Ile 15 tca aag gca aaa acc ttt gtg gga tgc gca ggc acc ttc acc aca atc 211 c) Ser Lys Al a Lys Thr Phe Val G y Cys Al a y Thr Phe Thr Thr Ile 30 tcc gcc tgg gtg caa ggc cta gaa agc tac gac cgc gac gcg atc cac 259 Ser Ala Trp Val n G y Leu u Ser Tyr Asp Arg Asp Ala lie His 45 CK1 ctc tct gca ctc aac ttc gat gca ctg cga gtt gtc acc gat gag atc 307 0 Leu Ser Ala Leu Asn Phe Asp Ala Leu Arg Val Val Thr Asp G u Ile 00 55 60 O 0 att tca gaa tca tca tca cag cgc gcc agc aac cca gtt gtt gat cca 355 C( Ile Ser u Ser Ser Ser G n Arg Ala Ser Asn Pro Val Val Asp Pro 70 75 80 0 ggt cgc gcc gac gtc atc ggt ggc gga tcc gtt gtt gtc caa gca gcg 403 C G y Arg Ala Asp Val Ile G y G y y Ser Val Val Val n Ala Al a 95 100 atc gac tta gcc tcc aaa gaa gcc ggt gta gac tac atc att att tcc 451 Ile Asp Leu Ala Ser Lys G u Ala G y Val Asp Tyr lie lie lle Ser 105 110 115 gaa aaa gac atc ctc gac ggc ctc atc ctt ggc ctg gta gaa gcc gac 499 G u Lys Asp Ile Leu Asp y Leu Ile Leu y Leu Val G u Ala Asp 120 125 130 tct ttg aag aaa taggacccta gttttaaacc act 534 Ser Leu Lys Lys 135 <210> 338 <211> 137 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 338 Val u Ile Ala Arg Asp Tyr Val Ala u Arg Ile On u Val Lys 1 5 10 Ala I e Val Pro Ile Ser Lys Ala Lys Thr Phe Val y Cys Ala y 25 Thr Phe Thr Thr lie Ser Ala Trp Val G n y Leu u Ser Tyr Asp 40 Arg Asp Ala lie His Leu Ser Ala Leu Asn Phe Asp Ala Leu Arg Val 55 Val Thr Asp u Ile Ile Ser u Ser Ser Ser n Arg Ala Ser Asn 70 75 Pro Val Val Asp Pro y Arg Al a Asp Val Ile y y y Ser Val 90 Val Val G n Ala Ala I e Asp Leu Ala Ser Lys G u Ala y Val Asp 100 105 110 Tyr Ile Ile Ile Ser u Lys Asp Ile Leu Asp y Leu Ile Leu y 115 120 125 Leu Val G u Ala Asp Ser Leu Lys Lys Page 405 seqI i st car r ect ed. t xt 130 135 <210O> 339 <21 1> 1497 <21 2> DNA <21 3> Cor ynebact er i urn gi ut anm cumn <220> <221 CIDS <222> (1474) <223> RXN00663 <400> 339 ct gaacgat t ggt gaccggc t cat gaaaac t tgacgagt c actactaccg tgggcgacaa gcccacttag aggaggactt cccggt at t c gccagcggt g cca Pr o g aa G u ac g Th r c ac Hi s cgt Ar g ct g Le u at t I Ie ac g Th r at c I Ie ct g Le u 1 50 at c I Ie tt c Ph e g aa G u gt g ValI gt a ValI c ac Hi s gag 0Ou g at As p t cc Ser 120 gca A a t cg Ser gca A a gga G31y ggt G y ct g Le u ct g Le u g ac As p cgt Ar g ggc G31y ccc Pr o gcc A a g ac As p g aa (31u 1 70 aat As n gcg A a t ct Ser cca Pr o ct t Le u 75 gag (31u gga G31y gt g ValI aag Ly s ct g Le u 155 t ac Ty r t ct Ser c ag G n g at As p at t I Ie ggc 0Gy at c I Ie c ac Hi s gga 0Gy aat As n 140 ccg Pr o cgc Ar g t cg Ser g aa G u ccg Pr a 45 gt t ValI tt t Ph e cat Hi s at c I Ie tt c Ph e 125 ct g Le u at g vb t gct A a ct c Le u gt c ValI tt g Le u gt a ValI tt t Ph e ggg G31y cat Hi s 110 cgc Ar g c ag O31n Ar g gcc A a ac c Th r 1 90 c aa O31n 15 t ct Ser t cg Ser at t I Ie gct A a g at As p 95 gt t ValI ct t Le u g aa O31u at t I Ie ct g Le u 1 75 g at As p ac a Th r gt t ValI tt g Le u ac g Th r cgc Ar g tt g Le u gag O31u ggt G31y gag O31u aag Ly s 1 gcg A a g ac As p gt g aca Val Thr 1 gat acg Asp Thr act t at Thr Tyr aca cgg Thr Ar g gaa tt a O3 u Leu caa gcg O3 n A a t cc aag Ser Lys ct g aat Leu Asn gac aat As p As n 130 ggc cac O3 y Hi s 145 gcg t cg A a Ser cgc gac Ar g As p cag ct c O3 n Leu gag O31u gt t ValI tt c Ph e g aa O31u ct t Le u cca Pr a c ac Hi s 115 g ac As p aat As n gca A a cgt Ar g ag c Ser 1 c ac Hi s tt g Le u gcg A a gcc A a cgg Ar g ct g Le u 1 00 c aa O31n ac c Thr gt g ValI ag c Ser ggt G31y 1 gag (31u acc acc t at Thr Thr Tyr acc ggc at g Thr 03 y I'bt cac gcc aat atc Hi s Al aAsn Il e Page 406 seqi i st car r ect ed. t xt at t gag gag ct c gaa aag ct g ccc gt c t ac gac acc ggc gag gt g cgc Tyr Asp Thr Gy Ou Val Arg 200 I I e G3 u G3 u L eu G3 u 205 aac As n at g vb t 230 tt c Ph e ct t Le u ac a Thr gag G u gt t ValI 310 at g vb t ag c Ser ct c Le u at c I Ie ct g Le u 390 gcg A a gt g ValI c ag O3n ggc (31y t cc Ser at c I Ie g ac As p aaa Ly s 280 cgc Ar g gga G31y cct Pr o aac As n ct t Le u 360 g ac As p cgc Ar g cgc Ar g g aa (31u t cc Ser 440 ac c Thr aag Ly s ggg G31y 250 gcc A a gca A a c ag (31n g aa (31u gcg A a 330 at c I Ie c ac Hi s gcc A a ggc G31y aac As n 410 ct c Le u cgc Ar g aaa t cc Lys Ser 220 at c gag I Ie O31u agc gct Ser A a ggc at c G31y I Ie gca ct g A a Leu 285 aag att Lys I I e 300 ggc t ac a3 y Ty r gcg gtt A a Val gaa gcc 03 u A a cgc gga Ar g G31y 365 t cc ct a Ser Leu 380 aat t cc Asn Ser cgc gtt Ar g Val gat cac As p Hi s cga at c Ar g I Ie 445 aac As n ct t Le u gag (31u gt a ValI 270 t gt cys at c I Ie ct g Le u tt t Ph e ct c Le u 350 cgc Ar g g aa (31u cga Ar g ggt G31y g aa (31u 430 gct A a ggt t cg 03 y Ser 225 ccc ggc Pro G31y 240 cgg gtt Ar g Val at c ggc I Ie G31y ggc ct g 03 y Leu ttc cgc Phe Ar g 305 ggc gac 31y As p 320 acc ct a Thr Leu cgc ggc Ar g G31y ct C cac Leu Hi s aac gt g As n Val 385 gt t ct c Val Leu 400 t ac gac Ty r As p ttt ggc Phe 03 y t tg gt c Leu Val Ly s 210 gcg A a g ac As p gcc A a ggc G31y g aa O31u 290 cca Pr o c aa O31n ag c Ser ct c Le u g at As p 370 tt g Le u ac c Thr ggc G31y c ac Hi s ct t Le u c ag O31n at t I Ie ccc Pr o 275 gct A a ct c Le u g aa O31u t cc Ser at c I Ie 355 gct A a ct c Le u cat Hi s at c I Ie at c I Ie 435 ggt (31y c ag O31n g at As p 260 gcg A a gt g ValI tt t Ph e g aa O31u at g vb t 340 g aa O31u tt c Ph e ac c Thr g ac As p gt c ValI 420 ac g Thr Leu Pr o Val cgt Ar g gt a ValI 245 tt g Le u ggt O31y at g vb t gcc A a aaa Ly s 325 gt c ValI gt t ValI gt c ValI at g vb t gt a ValI 405 t ct Ser tt g Le u 739 787 835 883 931 979 1 027 1 075 1123 1171 1219 1267 1315 1363 1411 1459 1497 acc caa gt t tt g Thr 03 n Val Leu 450 gat gcg As p A a 455 ccg t ct ct g t agt cgcgca gt ct gtggcg at t Pr o Ser Leu Page 407 seqi i st car r ect ed. t xt <210O> 340 <21 1> 458 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 340 Val Thr Thr As p Th r Th r O31u O31n Ser Le u As p G31y 145 A a Ar g O31n O31u Ser 225 O31y ValI O31y Le u Ar g 305 O31u ValI Ph e O31u Le u Pr o Hi s 115 As p As n A a Ar g Ser 1 95 Le u Le u O31n I Ie Pr o 275 A a Le u Thr Tyr Pro Asp His Trp Ou l'bt Leu As p Thr Ser Al a 03 u Hi s As p Ala Lys Ar g Hi s 70 Ar g l'b t Leu As p Leu Pr o I Ie (31y 1 00 03 n As n Thr 03 y Thr Ar g I Ie Leu 135 Val Val Leu Val 150 Ser 03 y I I e A a 1 65 O3 y Ty r Thr 03 y 1 80 O3 u Leu Ty r As p Pr o Val As n Hi s 215 O31y Ar g [Vb t As n 230 O31n Val Phe O31y 245 Asp Leu Leu Asn 260 A a 03 y Thr 03 y Val 'b t O31u Ar g 295 Phe Ala Val (31y 310 Ph e O31u ValI 40 ValI Hi s O31u As p Ser 120 A a Ser A a vb t Thr 200 O31y Ser I Ie As p Lys5 280 Ar g O31y Le u O31y Le u ValI Pr o Le u (31u 1 Le u ValI Lys5 O31n Thr 1 O31y Ph e As p Ser As p 265 Ser I Ie O31n O31y 10 O31y Le u Le u As p Ar g 90 G31y Pr o A a As p O31u 1 70 Hi s O31u Thr Lys5 O31y 250 A a A a O31n O31u As n A a Ser Pr a Le u O31u G31y ValI Lys5 Le u 1 Tyr A a ValI Le u I Ie 235 Ar g ValI Le u Ar g Le u 315 Ser O31n As p I Ie O31y I Ie Hi s O31y As n 140 Pr a Ar g As n Ar g Ly s 220 li1e Ser O31y A a Ly s 300 O31y Ser O31u Pr a ValI Ph e Hi s I Ie Ph e 125 Le u vb t A a le le 205 Ser O31u A a I Ie Le u 285 li1e Ty r O31n Ser Ser I Ie A a As p ValI Le u Ou le Le u 1 As p O31u Ser ValI O31n 255 Se r A a ValI Pr a Asp On OuOu Lys l'bt Oy Pro Trp Aia3n AiaVai PheAspThr Page 408 seql i st cor r ec 325 330 Leu Ser Ser IVet Val Ser n Asn lie lie Asp 340 345 Sy Leu Ile u Val Leu Pro Leu Thr His lie 355 360 His Asp Ala Phe Val Ile Val Asp G u Ala G n 370 375 Val Leu Leu Thr IVet Leu Ser Arg Ile G y G n 385 390 395 Leu Thr His Asp Val Al a G n Arg Asp Asn Leu 405 410 Asp Gy Ile Val Ser Val Val G u Ala Leu Lys 420 425 G y His lie Thr Leu G n Arg Ser G u Arg Ser 435 440 Val Thr G n Val Leu Asp Ala Pro Ser Leu 450 455 <210> 341 <211> 1248 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1225) <223> RXN00778 <400> 341 aggtcttagg tttttaagtc gtgagcaatc cggagggaaa gctcagacga tgtct t cact taaaccggaa aggct t cccc ted. txt G u Al a Arg G y 365 Ser Leu 380 Asn Ser Arg Val Asp Hi s Arg Ile 445 335 Ser Ser Ar g Val Ar g 415 Leu G u aag Lys ctt Leu gca Al a ggt Gy gca Al a tct Ser t cc Ser gct Al a t cc Ser acc Thr gac Asp gct Al a gcc Al a t cc Ser ggt Gy cag G n ttt Phe acc Thr gt g Val t cc Ser t cc Ser gtt Val at c SIle t ca Ser ggc Sy aat Asn gat Asp 45 gct Al a cgt Ar g ggt G y gca Al a gag G u gct Al a gaa G u t ac Tyr t cc Ser gtt act Val Thr 15 tct gat Ser Asp gca t cc Al a Ser ggt gca G y Al a t cc gag Ser G u 80 ggt tcc G y Ser 95 Page 409 ctagcccgcc tacaggatct gtg aac ctc act ctt Val Asn Leu Thr Leu 1 gca ggc tcc ttc gct Ala y Ser Phe Ala t cc acc tcc tca tct Ser Thr Ser Ser Ser att gag ggc ctt tcc ile G u G y Leu Ser tct tcc cag cag tcc Ser Ser G n G n Ser gct gtc agc ggt gca Ala Val Ser y Ala ggc cgc acc aac ttc Sy Arg Thr Asn Phe 100 eql i ggc gct gca ggc cag gtt gct ttc ggt Ala Ala Gy n Val Ala Phe y 105 st cor r ect ed. t xt tcc gac tcc gca atg aag gac Ser Asp Ser Ala IVet Lys Asp gac Asp cac Hi s ggc Sy 150 aag Lys gag G u gaa Gu acc Thr t cc Ser 230 gag G u ct g Leu gct Al a ggc Sy gcc Al a 310 gca Al a gtt Val cca Pr o gct Al a 120 cca Pr o gac Asp gag G u acc Thr t cc Ser 200 at c SIle gcg Al a gca Al a gtt Val t cc Ser 280 aac Asn gct Al a t ac Tyr ct g Leu acc Thr 360 gaa G u gtt Val ct g Leu acc Thr 170 ct c Leu acc Thr gag G u ggc Sy acc Thr 250 aac Asn ggc Sy gtt Val cca Pr o gag G u 330 t cc Ser gag G u gca Al a at c SIle aac Asn 155 aag Lys cca Pr o t cc Ser acc Thr t cc Ser 235 tac Tyr at c SIle gtt Val gtt Val ct g Leu 315 acc Thr cag G n cac Hi s gaa G u ggc G y 140 ct g Leu tgg Tr p gac Asp gac Asp gaa G u 220 aac Asn gtt Val gac Asp gca Al a gac Asp 300 at c SIle acc Thr gat Asp t ac Tyr gca Al a 125 cca Pr o gac Asp aac Asn cag G n aac Asn 205 ggc Gy ggt Gy gaa G u ttc Phe ctt Leu 285 acc Thr ct c Leu cgc Ar g gac Asp gat Asp 365 u y 110 cgt Ar g gtt Val acc Thr gac Asp gac Asp 190 ttc Phe cag G n gt a Val gct Al a ggt Sy 270 ggt Sy gac Asp acc Thr gac Asp cag G n 350 cgc Ar g tgc Cys gca Al a aac Asn gaa G u 175 at c SIle cag G n cag G n gct Al a ggt Sy 255 t cc Ser gca Al a gct Al a acc Thr cag G n 335 ct c Leu ct c Leu aac Asn gtt Val at c SIle 160 gca Al a t cc Ser aag Lys ttc Phe tct Ser 240 ttc Phe ggc Sy ct c Leu at g Met t ac Tyr 320 gt c Val gag G u gtt Val ggc Gy gct Al a 145 at c SIle at c l Ie gtt Val ttc Phe cca Pr o 225 gag G u gct Al a cca Pr o gac Asp ttc Phe 305 gaa G u aag Lys gct Al a gca Al a aac Asn 130 t ac Tyr gct Al a gct Al a ct g Leu ct c Leu 210 acc Thr gct Al a aac Asn gtt Val ttc Phe 290 gca Al a at c SIle gac Asp ct c Leu gca Al a 370 115 gaa G u aac Asn cag G n t cc Ser t ac Tyr 195 gga Gy gag G u t cc Ser cag G n gaa G u 275 ct g Leu at g Met gt c Val ttc Phe ggc G y 355 gtt Val gca Al a ct g Leu at c SIle cag G n 180 cgt Ar g gct Al a gtt Val aac Asn t cc Ser 260 ct c Leu act Thr aac Asn tgc Cys ct g Leu 340 t ac Tyr gaa G u tgg Tr p cct Pr o ttc Phe 165 aac Asn t cc Ser t cc Ser ggc Sy at c SIe 245 ggc Sy aac Asn gag G u gaa G u t cc Ser 325 acc Thr at c SIe gca Al a 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 Page 410 seqI i st car r ect ed. t xt at t cag t aat aaaccg ct gccgt agc tt c I Ie O31n 375 <210O> 342 <21 1> 375 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anri cumn 1248 <400> 342 Val Asn Leu Thr Al a Ser Ser Al a G31y Ser G31y Al a 145 le le ValI Ph e Pr o 225 O31u Al a Pr o As p Ser Ser O31y O31n Ser Th r vb t 115 O31u As n O31n Ser Ty r 1 95 O31y O31u Ser O31n O31u 275 Le u Leu Lys Arg Al a Leu Val Ser Al a Al a Ser 03 y Val Ser Al a I'b t 70 Al a Ser Leu Phe Al a Al a Asp Asp O31n Tr p Hi s Leu 135 Pro G31y Val 150 Phe Lys 03 y 1 65 Asn (1u (1y Ser 03 u 03 u Ser Thr Asp 215 O3 y Ser 03 y 230 Ilie O31u 31y 245 O3 y Leu 03 y Asn Al a 03 u O3 u 03 y Hi s 295 Ser Al a Ser 40 Th r As p Al a O31y Al a 120 Pr o As p O31u Th r Ser 200 li1e Al a Al a ValI Ser 280 As n I I e Al a Leu 10 Cys Ser As p Thr 03 y Ser O3 y 03 n Leu Ty r Phe O31y 75 Ty r Thr Pr o 90 O31n Val Al a 1 05 Al a 03 u Al a Phe Val I Ie Thr Leu Asn 1 55 Ilie Thr Lys 1 70 Asp Leu Pro 1 85 O3 y Thr Ser Trp O31u Thr O3 n 03 y Ser 235 Il e Thr Tyr 250 Al a Asn Il e 265 Val O31y Val I'b t Val Val ValI Ser Ser ValI li1e Ser Ph e O31u O31y 140 Le u Tr p As p As p O31u 220 As n ValI As p Al a As p 300 O31y As n As p Al a Ar g O31y O31y Al a 125 Pr o As p As n O31n As n 205 O31y O31y O31u Ph e Le u 285 Al a O31u Al a O31u Tyr Ser O31y 110 Ar g ValI Thr As p As p 1 Ph e O31n ValI Al a O31y 270 O31y ValI Ser Al a O31y Ser O31y Ser Cys Al a As n O31u 1 li1e O31n O31n Al a O31y 255 Ser Al a Thr As p Ser Al a O31u Ser As p As n ValI I Ie 1 Al a Ser Ph e Se r 240 Ph e O31y Le u Thr Asp Al a I'bt Page 411 seql i st cor r ect ed. t xt Phe Al a IVet Asn G u Al a y Al a Tyr Pro Leu Ile Leu Thr Thr Tyr 305 310 315 320 G u Ile Val Cys Ser Al a y Tyr Asp G u Thr Thr Arg Asp G n Val 325 330 335 Lys Asp Phe Leu Thr Val Ala Leu Asp Ser G n Asp Asp G n Leu G u 340 345 350 Ala Leu G y Tyr lie Pro Val Thr G y G u His Tyr Asp Arg Leu Val 355 360 365 Ala Ala Val u Ala lle n 370 375 <210> 343 <211> 870 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(847) <223> RXN00250 <400> 343 acaacaccag accaccccaa ccctgaataa acccctattt ttctaaaaag tcacactttg ccgtatagaa at t cagtcaa ccaagagtac tctgtccacc gcg Al a at g Met gcg Al a ct a Leu gct Al a gcc Al a cac Hi s t ac Tyr at c SIle t cc Ser ctt Leu ctt Leu ggt Sy cct Pr o at t l Ie cgc Ar g aag Lys 120 ttg Leu t cc Ser t cc Ser at t SIle tct Ser gca Al a ggc G y gt c Val act Thr cgt Ar g gtt Val t cc Ser ttt Phe ctt Leu ct g Leu cag G n cgt Ar g gat Asp gt g Val 140 gcg Al a ggc G y at c lle 45 ttc Phe tgg Tr p gt g Val ccg Pr o ttc Phe 125 ccc Pr o ct a Leu ccg Pr o gaa G u acc Thr ct g Leu ggt G y gat Asp 110 ttt Phe at c SIle ggt G y 15 ttc Phe t ca Ser ggt G y gt g Val t ac Tyr 95 ggc G y gag G u ccg Pr o ggt G y ggc G y ggt G y ct g Leu 80 tgg Tr p agg Ar g aag Lys atg gtt Mvt Val 1 tcc tgg Ser Trp agc ttc Ser Phe ct a ct t Leu Leu ct c ct a Leu Leu at c ct c I e Leu att ggc I le y att ttc Ile Phe cat ggc Hi s G y 130 ttt act ctt Phe Thr Leu ct g gac cct Leu Asp Pro att ctt ccg I e Leu Pro ttc cca ctt Phe Pro Leu gct aac cag Al a Asn G n tgc cct atc Cys Pro Ile cac aag ttc Hi s Lys Phe 100 aag cag gaa Lys G n G u 115 ccc gtg acg Pro Val Thr gtc cgt act tac gca cct ctg Val Arg Thr Tyr Ala Pro Leu 145 Page 412 gt c Val 150 at g Met gcg Al a ttg Leu cgc Ar g aac Asn 230 gcc Al a ggt G y ggt Sy ggt Sy at c SIle 200 ct g Leu gca Al a cag G n seql i st cor r ect ed. t xt gct ggc atg cgt tac cgc acg ttc att Ala G y Iet Arg Tyr Arg Thr Phe Ile 155 160 att ttg tgg ggt tcc ggc gtg gtg gct Se Leu Trp G y Ser y Val Val Ala 170 175 ttc gat ttc gtc cgc aac aat att gat Phe Asp Phe Val Arg Asn Asn Ile Asp 190 ttc att tcg gtg gtt cct ggt ttg gtc Phe Ile Ser Val Val Pro G y Leu Val 205 210 gac ggc cac aag caa gcc aac acc gag Asp G y His Lys G n Ala Asn Thr G u 220 225 cag aca gcc cca gta aaa acc cag gaa G n Thr Ala Pro Val Lys Thr G n u 235 240 taatctttcc ggtccgccag ttc at t SIle ttg Leu ct g Leu 195 ggc G y cca Pr o gcc Al a t ac Tyr ggt G y 180 at t SIle at g Met caa G n cag G n aac Asn 165 gct Al a ttc Phe gcc Al a gaa G u gaa G u 245 <210> 344 <211> 249 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 344 IVet Val Phe Thr Leu Al a Asp Ser 1 5 Ser Trp Leu Asp Pro IVet G u Leu L Ser Phe Ile Leu Pro Ala IVet Leu A 40 Leu Leu Phe Pro Leu Leu Pro Gy A Leu Leu Ala Asn G n Al a Asp Pro F 70 Ile Leu Cys Pro Ile Ala Ala lle L IIe G y His Lys Phe His Pro Arg L 100 1 Ile Phe Lys n G u Tyr Leu Lys C 115 120 His G y Pro Val Thr IIe IIe Leu C 130 135 Thr Tyr Ala Pro Leu Val Ala y N 145 150 Phe lie lie Tyr Asn Iet Ile G y C 165 /al Ser 10 eu Ser la I e sp Ser 'he Al a .eu G y 90 .eu Val Sn Thr .ys Arg /et Ala 3y I le G n Sy Val Leu Pr o 75 Asp Asn G u Phe Sy 155 Al a Gy I e Phe Tr p Val Pr o Phe 125 Pr o Ar g G y Pro Phe G y Ser G y G y G y Val Leu Tyr Trp G y Arg G u Lys Val Arg Arg Thr 160 G y Val 175 Leu Trp Gy Ser Page 413 seql i st cor r ect ed. t xt Val Ala Leu Gy Ala Al a Leu Gy G n Phe Asp Phe Val Arg Asn Asn 180 185 190 Ile Asp Leu Ile Phe Leu Leu lie Val Phe Ile Ser Val Val Pro y 195 200 205 Leu Val G y IVet Ala Arg Lys Leu Ala Asp G y His Lys G n Ala Asn 210 215 220 Thr G u Pro n G u Asn Pro Ala Val G n Thr Ala Pro Val Lys Thr 225 230 235 240 Gn G u Ala Gn G u Ala Pro Gn Asn 245 <210> 345 <211> 541 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(541) <223> RXA00072 <400> 345 acggccagga cgatccagtg cacaggccag caccagcaaa gtccacatcg caagcat t aa aagaatctct cgaaagacac aaaagaggtg gtt Val ccc Pr o cat Hi s agc Ser at t I e gcg Al a cac Hi s t cc Ser gat Asp gcc Al a gga Sy gag G u caa G n gt g Val cac Hi s aag Lys aag Lys 120 att SIle aaa Lys cgc Ar g ct c Leu at c SIle ttg Leu ccg Pr o acc Thr gt c Val ggt G y act Thr acc Thr gaa G u gaa G u at g Met gct Al a gaa G u gcg Al a aac Asn ggt G y aca Thr aag Lys 45 tgg Tr p gaa G u gat Asp gtt Val ct g Leu 125 ct g Leu agtcgcaaca atg agc ttt IVet Ser Phe 1 tcg gta aaa gat ccc gag Ser Val Lys Asp Pro G u 15 ccg ttg tca cca gag gta Pro Leu Ser Pro G u Val cat gct gct gcg ttg tat His Ala Ala Ala Leu Tyr aca gcc gag cac gcg ccg Thr Ala G u His Ala Pro aac acc gtg ctg gcg gag Asn Thr Val Leu Ala G u 80 ttc ctc ttt ttg gac acc Phe Leu Phe Leu Asp Thr 95 gcc cgt cag gta gat gag Ala Arg n Val Asp u 110 115 ccg atc ctc aag cgc acg Pro lie Leu Lys Arg Thr 130 tac cgc agc aac cca gcg Tyr Arg Ser Asn Pro Ala Page 414 caa G n at c I e gca Al a gac Asp ggc Sy ct g Leu ggt Sy 100 cgc Ar g gag G u ct a Leu t ca Ser aaa Lys gcc Al a gct Al a gct Al a t ac Tyr t at Tyr cag G n seql i st cor r ect ed. t xt 135 140 <210> 346 <211> 147 <212> PRT <213> Cor ynebact eri um gl ut ai cum <400> 346 IVet Ser Phe G n Leu Val Asn Al a Leu Lys Asn 1 5 10 Asp Pro G u lie Ser Pro G u y Pro Arg Thr 25 Pro G u Val Ala Lys His Asn G u G u Leu Val 40 Ala Leu Tyr Asp Ala Ser Ala n G u lie Leu 55 His Ala Pro G y Ala lIe Ala Val Thr Leu Ser 70 Leu Ala G u Leu Ala Al a Arg His Leu Pro G u 90 Leu Asp Thr G y Tyr His Phe Lys G u Thr Leu 100 105 Val Asp G u Arg Tyr Ser G n Lys Leu Val Thr 115 120 Lys Arg Thr G u G n Asp Ser Ile Tyr y Leu 130 135 Asn Pro Al a 145 <210> 347 <211> 1299 <212> DNA <213> Corynebact er i um gl ut am cum <220> <221> CDS <222> (101)..(1276) <223> RXA00793 <400> 347 tcgctggttt ttagatggtt ttcaagccag cgagaccaca acctttgaga tcaatataga ccgtgtggtc tactcgagga 145 Thr Thr G u G u Met Al a G u Al a Asn 140 Gy Thr Lys Tr p G u Asp Val Leu 125 Leu Ser Pr o Hi s Thr Asn Phe Al a 110 Pr o Tyr Val Leu Al a Al a Thr Leu Ar g SIe Ar g ccc act acc Pro Thr Thr at c aca gcc I le Thr Al a gca gac ct c Al a Asp Leu ttg cac tgg ttc cta ccc Leu His Trp Phe Leu Pro ggc ggg cat ggc ttc ggc G y G y Hi s G y Phe G y 30 gat tac ctc tcc caa att Asp Tyr Leu Ser n lie 45 acc t at Thr Tyr 15 ttc cac Phe Hi s gcc ctg Al a Leu Page 41 ttagtttcac gctggttgaa atg agt gaa aac aat IVet Ser G u Asn Asn 1 ggc gat tct cgc gga Gy Asp Ser Arg y tcc gga agc cgg aca Ser G y Ser Arg Thr gcc gct gaa cga aac Ala Ala G u Arg Asn seql i st cor r ect ed. t xt ggt G y tgg Tr p ct c Leu cag G n gt c Val tct Ser at c SIle 150 ttc Phe cca Pr o gcc Al a gt a Val cgc Ar g 230 gag G u cag G n gaa G u aca Thr gca Al a 310 gaa G u acc Thr gct Al a gct Al a gt g Val 120 aaa Lys cac Hi s agt Ser at t SIle cat Hi s 200 gag G u ct a Leu gaa G u gag G u cag G n 280 gga G y gtt Val gt c ct g act Val Leu Thr gca gcg ctg Al a Al a Leu 75 cga cca ggc Arg Pro G y ttc cag aaa Phe G n Lys ggc gaa gac Sy G u Asp gag cgc tac G u Ar g Tyr 140 tgg aac agc Trp Asn Ser 155 gaa aac gcg G u Asn Al a 170 ttt ggc gga Phe G y G y gat gtt tat Asp Val Tyr ctt gcc cgg Leu Al a Ar g 220 tat ggc at c Tyr G y Il e 235 tgg tca gt g Trp Ser Val 250 gcc cgc att Al a Ar g Il e cgc atg acg Arg IVet Thr gat gct cgc Asp Al a Ar g 300 ct a gt c cgc Leu Val Arg 315 ccc act gga Pro Thr G y ct g Leu caa G n ttc Phe cat Hi s 125 cac Hi s gca Al a gt a Val t cc Ser ct c Leu 205 gt g Val cgc Ar g gct Al a cag G n gaa G u 285 t cc Ser ggt Gy t ct Ser gtt Val t ca Ser 110 gaa G u aag Lys gaa G u ttg Leu t ca Ser 190 acc Thr cgc Ar g ct g Leu caa G n gaa G u 270 ctt Leu ctt Leu ggc Gy agg Ar g agc Ser 95 aat Asn cag G n gct Al a cct Pr o aag Lys 175 caa G n tgg Tr p gcc Al a cat Hi s aat Asn 255 ggg G y cat Hi s gaa G u gcc Al a ttg tgg tgc Leu Trp Cys aca tca aaa Thr Ser Lys 80 ccc acc at c Pro Thr Il e aac cgc ct g Asn Arg Leu cgc gct ttc Arg Ala Phe 130 gat gaa acc Asp G u Thr 145 cta aat ttc Leu Asn Phe 160 gaa cag ccc G u G n Pr o ct c ggc at c Leu G y Ile ggt gaa cct G y G u Pr o 210 gaa gca gat G u Al a Asp 225 gtc att gct Val Ile Al a 240 ctt ctt gac Leu Leu Asp ctt gcg cgt Leu Al a Ar g gga caa ggg G y G n y 290 att gca ccg I e Al a Pr o 305 ggc aca gcg G y Thr Al a 320 gaa G u ct g Leu at c SIle ct c Leu 115 gct Al a tta Leu cag G n gag G u gaa G u 195 gcg Al a aag Lys cga Ar g caa G n tct Ser 275 gca Al a aat Asn ttg Leu gat Asp aaa Lys gcg Al a 100 at c SIle gat Asp gag G u ggt G y gtt Val 180 at c SIle gaa G u cga Ar g cca Pr o ctt Leu 260 caa G n gca Al a ct c Leu gt g Val gcg Al a ttc Phe cag G n aac Asn t at Tyr at c SIe gaa G u 165 t cc Ser gca Al a aag Lys aac Asn act Thr 245 gat Asp tcg Ser ttc Phe tgg Tr p ggt Gy 325 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 Page 416 seqI i st car r ect ed. t xt t cc tat gag caa gt cgcg caa gca att t t gcga tac cgc gat at t ggt Ser Tyr O31u O31n Val Ala O31n Ala Ilie Leu Ar g Tyr Ar g Asp Ilie (31y 330 335 340 ct g agc cac t tc at t t tc t cc ggc t at cca cat t tg gag gaa acc t at Leu Ser Hi s Phe Ilie Phe Ser O31y Tyr Pr o Hi s Leu O31u O31u Thr Tyr 345 350 355 cac gt g ggc gaa gga gt g gt a cct gag ct c ct c aaa t tg ggt gt t ccg Hi s Val O31y O31u O31y Val Val Pr o O31u Leu Leu Lys Leu O31y Val Pr o 360 365 370 gtg aac aac cat gaa gaa caa cgc aac gac gtg gta gcg act ccg ttt Val Asn Asn Hi s O31u O31u O31n Ar g Asn Asp Val Val Ala Thr Pr o Phe 375 380 385 at t t cc aga t agat cacgg at cggct gct tt a Ilie Ser Ar g 390 <210> 348 <211> 392 <21 2> PRT <21 3> Cor ynebact er i urn gl ut ari cum 1123 1171 1219 1267 1299 <400> 348 I'bt Ser 03 u Asn Asn Pro Thr Thr Le u G31y Ser A a Tr p Ser Thr Ar g A a (31u 145 As n O31n G31y Ser Ser O31u O31u Le u I Ie Le u 115 A a Le u O31n O31u O31u 1 95 O31y Thr As n A a Ph e O31n As n Tyr I Ie O31u 1 65 Ser A a Thr As p Ph e li1e ValI O31y ValI Ser 135 As p Le u Pr a O31n A a 03 y Leu Asp 40 O3 u Ser Thr Thr A a I I e A a A a 1 Val O31y 120 Lys 03 u Hi s Leu Ser Val I I e Ty r 1 Hi s Ser 200 Hi s 10 O31y Tyr ValI A a Ar g 90 Ph e O31y O31u Tr p O31u 1 70 Ph e As p Tr p Hi s Le u Le u A a Pr a O31n O31u Ar g As n 1 As n O31y ValI Ph e O31y Ser Thr Le u O31y Lys5 As p Tyr 140 Ser A a O31y Tyr Le u Ph e O31n Pr a Le u O31n Ph e Hi s 125 Hi s A a ValI Ser Le u 205 Thr Ph e A a O31y Ar g Ser As n O31n A a Pr a 1 O31n Tr p Ou Pro Ala Ou Lys Val Ou Ou Lys Leu Ala Arg Val Arg Ala Ou Page 417 seql i st cor r ect ed. t xt 215 G u Leu Asp Tyr Al a 225 I le Leu Al a G n Al a 305 Thr Tyr Leu Lys Val 385 Lys Ar g G n Ser 275 Al a Asn Leu Asp G u 355 Gy Thr Asn Thr 245 Asp Ser Phe Tr p Gy 325 Gy Tyr Pr o Phe Asp G u Gy Al a 295 Gy Tyr Ser Val Asn 375 Ser Al a Val 265 Ar g Al a Gy G n Phe 345 G u Hi s Tr p 250 Al a Ar g Asp Leu Val 330 I le Gy G u Gy 235 Ser Ar g et Al a Val 315 Al a Phe Val G u 220 li e Ar g Val Ala Ile G n Thr G u 285 Arg Ser 300 Arg G y G n Al a Ser G y Val Pr o 365 G n Arg 380 Hi s Asn 255 Sy Hi s G u Al a Leu 335 Pr o Leu Asp <210> 349 <211> 681 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(673) <223> RXA01192 <400> 349 ccacggtgaa cacctgcagg tggagcaagc tagccttgcg ggagattctt tttggtggat cgtcgccagc tgcaggtgag gcg Al a aaa Lys cgc Ar g gca Al a acc Thr aac Asn gga G y gt g Val ct c Leu at c SIle cgc Ar g gca Al a tgg Tr p gag G u cat Hi s aag Lys ggt G y ct a Leu gt g Val 45 ttg Leu gaa G u gca Al a at c SIle at t SIle act ccc Thr Pro 15 gca cag Al a G n acc cgc Thr Arg agc ggg Ser G y Page 41 catccgccag agattattcc gtg gct gca cgt tat Val Ala Ala Arg Tyr 1 gat cag gtg gcg cag Asp n Val Ala n cgc ggc cgg gaa ctg Arg y Arg G u Leu gat acg tct gaa gaa Asp Thr Ser G u G u gtc act cca gaa cag Val Thr Pro G u G n gt c ValI cgc Ar g t ca Ser ggt O31y gag O31u tt t Ph e 1 50 gag O31u c aa O31n aag Ly s at g vb t gct Al a ct t Le u 120 gcc Al a ct g Le u gt g ValI t ca Ser c aa O31n gag O31u g at As p gga O31y cgc Ar g ggt O31y ccg Pr o 1 70 gt t ValI gcc Al a 75 ct g Le u ct g Le u ggt O31y at c I Ie t at Tyr 155 aaa Ly s t t g Le u ggg O31y c ac Hi s gag O31u gca Al a g aa O31u 140 cca Pr o ct g Le u g aa O31u seql i st car r ect ed. t xt ttt gca acg t ct aag t cg Phe Al a Thr Ser Lys Ser 80 agc aag ggt cgt gcc ttt Ser Lys O31y Arg Al a Phe 95 gt g t at ccc aat gt g t gg Val Tyr Pr o Asn Val Tr p 110 gga aca gcc ctt gtg ggc O31y Thr Al a Leu Val O31y 125 130 gaa t ac gca gca ct C ggc O3 u Tyr Al a Al a Leu 03 y 145 aac t tg gag gag gcc tt c Asn Leu 03 u 03 u Al a Phe 1 60 ct g cgc cgc ggt gt g gat Leu Ar g Ar g O31y Val Asp 1 75 cct gt t ggg taaacggg Pr o Val O31y 1 ggg O31y agt Ser 1 00 ggc O31y cat Hi s g at As p tt c Ph e aaa Ly s 1 c ag O31n ggc G31y gt c ValI g aa (31u c ag O31n ggt O31y 1 aat As n <210O> 350 <21 1> 191 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 350 ValI Al aAla Arg Tyr Ala Asp Thr T 1 5 Asp O31n Val Ala O1n Lys lieAsn T Ar g O31y Ar g O31u L eu Ar g Hi s (31y 1 40 As p Thr Ser Ou Ou Ala Trp Val\ 55 Val Thr Pro O31u O31n VaI Al a Lys A 70 Lys Ser O31u O31y O31n Ar g Ar g Ib t P Al a Phe Thr Ser 03 y Se r Thr Al a P 100 1 Val Tr p Al a O31y Va 1O31y Leu Leu P 115 120 Val O31y Ser Hi s O31u O31u Val Al aA 130 135 Leu O31y Leu As p O31n Ph e Val Leu S yr Leu Thr Trp Oy Ou Thr Pro r p 25 1le Il a Il a ~r g 05 ~r g 0 p ie r 10 I I e As n 03 u Ar g Phe Hi s Al a 03 u Lys O3 n Al a 03 y O3 u Leu Hi s 90 Asp Leu 03 u O3 y 03 y Al a Arg Ilie O31u 140 O31y Tyr Pr o Page 419 Le u ValI Le u Ph e Ser ValI O31y 125 O31u As n Al a li1e I Ie Al a Lys5 Tyr 110 Thr Tyr Le u Al a Thr Ser Th r O31y Pr a Al a Al a O31u seql i st cor r ect ed. t xt 145 150 155 160 Ala Phe His Phe G y G u y Val IIe Pro Lys Leu Leu Arg Arg y 165 170 175 Val Asp I e Lys Asn n G u Ser Arg Val Leu G u Pro Val G y 180 185 190 <210> 351 <211> 918 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(895) <223> RXA00715 <400> 351 gtggtgttaa gcactaagat ggcaggttat gacttctctt tgcaaccaat aacaatgatg cccattttcc tgaaggtcca aaagtaactt cgtccgcaga gtg gt a Val Val acc gtt gac Thr Val Asp tgg Tr p aca Thr ttt Phe cca Pr o at t SIle gtt Val at t SIle cca Pr o gaa G u at c l Ie 150 ttc Phe t ca Ser gag G u gct Al a acc Thr acc Thr ccg Pr o gt g Val gaa G u 120 cag G n cgc Ar g ggc Gy aac Asn gat Asp ttg Leu cca Pr o t cc Ser gt g Val gat Asp ct g Leu gat Asp agc Ser gaa Gu ctt Leu gaa Gu gaa Gu cca Pr o acg Thr 75 tgg Tr p ggc Gy tcg Ser tta Leu aag Lys 155 gaa Gu gac Asp at t SIle gga Gy aat Asn gt g Val tgg Tr p gga Gy ct a Leu ct c Leu 140 gca Al a gag Gu cgg Ar g gca Al a gat Asp 45 ttg Leu gtt Val ctt Leu ttg Leu cct Pr o 125 gtt Val gt g Val ccc Pr o 1 gat gat gtc atc gtg ttg tgt gcc Asp Asp Val lie Val Leu Cys Ala 15 cgt caa gcg gga att ccg ggg gca Arg On Ala Gy lie Pro Gy Ala ttc tca gat cca cat tcc gag ctt Phe Ser Asp Pro His Ser G u Leu gtg ggt ttg cta gaa agc tac ggc Val y Leu Leu u Ser Tyr y gtt tat gat ctg cac ggc ctc atg Val Tyr Asp Leu His G y Leu IVet 80 ctc cgt gtt gct gga tta agc agc Leu Arg Val Ala y Leu Ser Ser 95 100 cca gcc tgg gtt gat gct ggc ctt Pro Ala Trp Val Asp Ala y Leu 110 115 aca agt ggt gga agg atc agc gca Thr Ser Gy Gy Arg Ile Ser Ala 130 ggt gcc tcc ggc gtt gaa cgg gcg Gy Ala Ser y Val u Arg Ala 145 att gat gct cgt aat gcg agc cga Ile Asp Ala Arg Asn Ala Ser Arg 160 165 cgt cca ggc ctt cga aaa ggg tcg Arg Pro y Leu Arg Lys y Ser Page 420 at c SIle ggt Gy aca Thr gca Al a 230 gt a Val aag Lys gga Gy gt c Val 200 gga Gy gtt Val t at Tyr at t SIe seql i st cor r ect ed. t xt 170 175 gtc aac att ccc ttc act gac att tct Val Asn lie Pro Phe Thr Asp Ile Ser 190 cca gca gaa gaa ctg aag gaa ttg atc Pro Ala u u Leu Lys u Leu Ile 205 210 cag tcg ttg gtc ttt agc tgt ggc tcc G n Ser Leu Val Phe Ser Cys y Ser 220 225 gcc tac gct gca gtt atc gca ggt tat Ala Tyr Ala Ala Val Ile Ala Gy Tyr 235 240 ggc tct tgg gcg gag tgg ggc aac ccg Sy Ser Trp Ala G u Trp y Asn Pro 250 255 taacgcccgc tatgataacc act gat Asp 195 ttc Phe gga Gy gac Asp gca Al a 180 gag G u agc Ser gtc Val gac Asp aac Asn 260 <210> 352 <211> 265 <212> PRT <213> Corynebacteri um gl utami cum <400> 352 Val Val Thr 1 I e Gy Pr o Leu Leu Al a Val Gy Gy 145 Ar g Leu Leu Pr o Ser Ser Gy Leu Al a 115 I le G u Al a Lys Val Asp Tr p 5 Cys Al a Thr G y Al a Phe G u Leu Pro Tyr G y Il e 70 Leu bet Val Ser Ser II e 100 G y Leu Pr o Ser Al a G u Arg Ala li e 150 Ser Arg Phe 165 G y Ser lie 180 Leu bet Leu Hi s Ser Al a Gy Thr Pr o 135 Al a Al a Pr o Ser G u Al a 40 Thr Thr Pr o Val G u 120 G n Ar g Gy Gy Hi s Asp Asp Al a Asp Ar g Leu 105 Pr o Pr o Ser Val Ser 185 Leu G u G u Pr o Thr 75 Tr p Gy Ser Leu Lys 155 G u Asp SIle Gy Asn Val Tr p Gy Leu Leu 140 Al a G u Ar g Al a Asp Leu Val Leu Leu Pr o 125 Val Val Pr o Asp G n Ser Gy Tyr Ar g Al a Ser Al a Asp Pr o 175 Val Al a Asp Leu Asp Val Tr p Gy Ser Al a 160 Gy Val Asn lie Pro Phe Thr Asp 190 Page 421 lie Ser Asp G u His G y Phe Val 195 200 Leu lie Phe Ser Arg Thr Asn y 210 215 Sy Ser y Val Thr Al a Cys Val 225 230 G y Tyr Asp Asp Val Val Val Tyr 245 Asn Pro Ala Asn G n Lys Pro Ile 260 eql i st cor r ect ed. t xt Arg Pro Ala G u u Leu Lys u 205 Ala G n Ser Leu Val Phe Ser Cys 220 Asp Ala Tyr Ala Ala Val I e Ala 235 240 Su y Ser Trp Ala u Trp y 250 255 Al a 265 <210> 353 <211> 945 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(922) <223> RXA01664 <400> 353 cgggttacca aagtgaatgg taggggaagt ttccgtgtct cgcgctgcgc ttggtcacat taacgcctag gctcggggct tataccggtt aggttttgcc acc gtg ttg Thr Val Leu tct Ser gtt Val ttc Phe gag G u ccg Pr o gat Asp cgc Ar g ctt Leu ggc Gy t cc Ser gct Al a tct Ser t cc Ser aat Asn gaa G u tgg Tr p ggt Gy 120 cct Pr o ctt Leu ttc Phe cac Hi s gtt Val ttg Leu gt g Val at c SIle ttc Phe aac Asn gct Al a tgg Tr p at c SIle cct Pr o gca Al a 75 ttt Phe ct c Leu cag G n tt c Phe gaa G u gct Al a cca Pr o t cc Ser cgt Ar g t ac Tyr cga Ar g aag Lys ccg Pr o t ca Ser cca Pr o act Thr 45 tct Ser tct Ser gat Asp tgg Tr p tgg Tr p 125 cac Hi s at c cac gct Ile His Ala 15 att gaa gga lie u G y tcc att ttc Ser Ile Phe gaa gat ggc Su Asp y ttc agg acc Phe Arg Thr 80 cag gga cgt Sn G y Ar g 95 gcg ggc atg Al a G y lt 110 gaa gac cat G u Asp Hi s ttt tgc aat Phe Cys Asn aag Lys ggc G y gac Asp aac Asn ggt Sy ctt Leu aac Asn ct g Leu 130 aaa Lys cgc Ar g cct Pr o cca Pr o ttg Leu ttt Phe gtt Val 115 gga Sy caa G n aca Thr gcc Al a ct g Leu aat Asn gct Al a 100 cgc Ar g cac Hi s att I e act Thr gtt Val ctt Leu cca Pr o acc Thr gca Al a at c SIe gct Al a gtg cgt ccc aac cca Val Arg Pro Asn Pro Page 422 seql i st cor r ect ed. t xt 145 ggt Gy 150 at t l Ie aag Lys gct Al a at c SIle at c SIle 230 at g Met t ca Ser ct g Leu at t SIle gat Asp tct Ser 200 cag G n t at Tyr cac Hi s tgg Tr p gt a Val tct Ser 170 aaa Lys ct g Leu ttt Phe ggt Gy ggg Gy 250 gct Al a gcg Al a 155 cgc Ar g gcc Al a gaa G u gcg Al a t cc Ser 235 ct a Leu aac Asn acc Thr gat Asp gga Gy gat Asp gac Asp 220 ggt Gy acc Thr ccc Pr o at c SIle gaa G u cac Hi s gat Asp 205 aag Lys aac Asn ggt ggy gag G u gaa G u caa G n at t SIle 190 ttc Phe ggg Gy cac Hi s gcg Al a aat Asn 270 gat Asp cga Ar g 175 cca Pr o acc Thr gt a Val tcg Ser agc Ser 255 cct Pr o gt c Val 160 ttt Phe ggc Gy ttc Phe acc Thr t cc Ser 240 cat Hi s at c SIle aag Lys gcg Al a gct Al a aaa Lys agc Ser 225 cag G n t at Tyr gag G u gca Al a ggt Gy at c l Ie t ca Ser 210 gga Gy ttg Leu ttt Phe gcc Al a cat cag ggc Hi s G n G y 165 cgc agt gaa Arg Ser G u 180 aac atc aac Asn Ile Asn 195 cca gaa gaa Pro G u G u gag aac gtc G u Asn Val ctg gct ggc Leu Al a G y 245 gct ggt tgg Al a G y Trp 260 t aaaat cgt g gcttgagtac gca <210> 354 <211> 274 <212> PRT <213> Corynebacter i um gl ut ari cum <400> 354 IVet Thr Val Leu lie Ser Pro Ser Thr 1 5 Sy Lys Lys G n Thr Val Leu Ala Ala 25 Ala y Arg Thr Val Phe Cys Ser G u 40 Cys Asp Pro Ala Leu G u Leu Ser y 55 Arg Asn Pro Leu Pro Pro Leu Asn Val 70 Trp y Leu Asn Thr Asp Arg u lie Sy Leu Phe Ala Ala Arg Ala Trp Trp 100 105 Pro Asn Val Arg Ile Leu Asp Gy Gy 115 120 G u Leu G y His Ala G y G y Pro G y Leu Al a G u 10 Phe Trp Al a His Ile Pro Val Pro Ser Leu Al a Ar g Val Phe Tyr 90 Ile Leu Ar g Phe G n Lys Asn Phe Pro Page 423 Ser Pr o Thr Ser Ser Asp Tr p Tr p 125 Hi s I e I e Ser G u Phe G n Al a 110 G u Phe Hi s Su I e Asp Ar g Gy Gy Asp Cys Val 145 Lys Al a Al a Lys Ser 225 G n Tyr G u Pr o Hi s Ar g Asn 195 Pr o G u Leu Al a Pr o Gy 165 G u Asn G u Val Gy 245 Tr p 135 G n Leu Leu Lys Ar g 215 Val G u G n seql i st cor r ect ed. t xt 140 Leu Ser Val Ala Thr Ile 155 Ile Asp Ser Arg Asp u 170 Asp Leu Lys Al a G y Hi s 185 Ser Leu Leu G u Asp Asp 200 205 G n I e Phe Ala Asp Lys 220 Tyr Ser y Ser y Asn 235 Hi s Al a G y Leu Thr G y 250 Trp Ser Ala Asn Pro G u 265 G u G n SIle 190 Phe Gy Hi s Al a Asn 270 Asp Ar g 175 Pr o Thr Val Ser Ser 255 Pr o <210> 355 <211> 746 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> <223> RXN02334 <400> 355 gtc aaa gac ctc aac gat ccc ctc acc Val Lys Asp Leu Asn Asp Pro Leu Thr 1 5 gct ttc gct gag ctg atg aac cgc aag Ala Phe Ala G u Leu IVet Asn Arg Lys gtt gtt gtc tac ggt gac aag tcc aac Val Val Val Tyr G y Asp Lys Ser Asn 40 tgg gtc ttc gaa ctg ttc ggc cac tcc Trp Val Phe G u Leu Phe Gy His Ser 55 ggc cgc gac gcg tgg atg gct gaa gag G y Arg Asp Ala Trp IVet Ala G u G u 70 ccg gag tac ccc tcc gcc aac tac ccc Pro G u Tyr Pro Ser Al a Asn Tyr Pro aac cag cgc gcg ttc gtg gct gag gtg Asn G n Arg Ala Phe Val Ala G u Val 100 105 cgc gat ttc Arg Asp Phe 10 ggc at c gct G y I le Al a tgg tgg gct Trp Trp Al a gat gtc cgc Asp Val Ar g cgc gac acc Arg Asp Thr 75 gt c gt g gag Val Val G u 90 ctc ggt tcg Leu G y Ser Page 424 at t SIle cgc Ar g gcg Al a ct g Leu t cc Ser cgt Ar g ct c Leu ggt Gy gac Asp acc Thr aac Asn gt g Val gac Asp caa G n gaa G u acc Thr ctg Leu ggc G y gtt Val gaa G u t cc Ser seql i st cor r ect ed. t xt ggt Gy ttg Leu gga G y 145 ccc Pr o gat Asp cgc Ar g aac Asn cgc Ar g 225 t ca Ser at g Met 115 gag G u at c SIle gga G y aac Asn gcc Al a 195 cga Ar g ccg Pr o acg ctt Thr Leu cac ggc Hi s G y cca ggc Pro y aac ttc Asn Phe 165 cca gct Pr o Al a 180 cac acc Hi s Thr aac t at Asn Tyr atc gaa Ile G u gt c Val aac Asn gcg Al a 150 cgc Ar g gac Asp tgg Tr p gac Asp act Thr gac Asp cca Pr o 135 at c l Ie acc Thr gat Asp ttc Phe gga Gy 215 ggc Gy gt c Val 120 acc Thr aac Asn cgt Ar g acc Thr gt g Val 200 tcg Ser gaa Gu agg acc cct tcg gag Arg Thr Pro Ser G u 125 tca aac acc ggc gt g Ser Asn Thr G y Val 140 ctg gat tgg tcg gac Leu Asp Trp Ser Asp 155 gca gag ttg gac aag Ala G u Leu Asp Lys 170 gtt gtc tac tgc cag Val Val Tyr Cys G n 185 ctg aag tat ctg ctc Leu Lys Tyr Leu Leu 205 tgg gca gaa tgg ggc Trp Ala G u Trp y 220 aac acc aaa aat aac Asn Thr Lys Asn Asn 235 t cc Ser cgt Ar g gtt Val t ac Tyr 175 G y ttc Phe at g Met tcg Ser gga G y ggt G y ctt Leu 160 gcc Al a gac Asp aac Asn gtt Val gtg Val 240 tagaataggc gtatcccctt ttt <210> 356 <211> 241 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 356 Val Lys Asp Leu Asn Asp Pro Leu Thr 1 5 Ala Phe Ala G u Leu IVet Asn Arg Lys Val Val Val Tyr G y Asp Lys Ser Asn 40 Trp Val Phe G u Leu Phe G y His Ser 55 G y Arg Asp Al a Trp IVet Al a G u u 70 Pro G u Tyr Pro Ser Al a Asn Tyr Pro Asn G n Arg Ala Phe Val Ala G u Val 100 105 G y G y bet Thr Leu Val Asp Val Arg 115 120 Arg Asp Phe 10 G y Ile Al a Trp Trp Al a Asp Val Ar g Arg Asp Thr Val Val G u 90 Leu G y Ser Thr Pro Ser Page 425 SIle Ar g Al a Leu Ser Ar g Leu G u 125 Sy Asp Thr Asn Val Asp G n Ser Leu Gy 145 Pr o Asp Ar g Asn Ar g 225 Ser Su I e Gy Asn Al a 195 Ar g Pr o Gy Gy Phe 165 Al a Thr Tyr G u Asn Al a 150 Ar g Asp Tr p Asp Thr 230 Pr o 135 l le Thr Asp Phe Gy 215 Gy seql i st cor r ect ed. t xt Thr Ser Asn Thr G y Val 140 Asn Leu Asp Trp Ser Asp 155 Arg Ala G u Leu Asp Lys 170 Thr Val Val Tyr Cys G n 185 Val Leu Lys Tyr Leu Leu 200 205 Ser Trp Ala G u Trp y 220 G u Asn Thr Lys Asn Asn 235 <210> 357 <211> 377 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> <223> FRXA02334 <400> 357 gga gtt ctc ccg gat tgg atg agc acg gca acc caa ccc Gy Val Leu Pro Asp Trp IVet Ser Thr Ala Thr G n Pro 1 5 10 ggc gtg ctt cgt ggt gaa cac atc cca ggc gcg atc aac G y Val Leu Arg y G u His lie Pro y Ala lle Asn 25 tcg gac gct gtt ctt ccc aac gga aac ttc cgc acc cgt Ser Asp Ala Val Leu Pro Asn Gy Asn Phe Arg Thr Arg 40 gac aag ctc tac gcc gat ctc aac cca gct gac gat acc Asp Lys Leu Tyr Ala Asp Leu Asn Pro Ala Asp Asp Thr 55 tgc cag gtt ggc gac cgc gcg gcc cac acc tgg ttc gtg Cys G n Val G y Asp Arg Ala Ala His Thr Trp Phe Val 70 ctg ctc ggt ttc aac aac gtc cga aac tat gac gga tcg Leu Leu Gy Phe Asn Asn Val Arg Asn Tyr Asp Gy Ser 90 tgg ggc aat atg gtt cgc atg ccg atc gaa act ggc gaa Trp y Asn Iet Val Arg IVet Pro Ile u Thr G y u 100 105 aat aac gtt tcg gtg tca tagaataggc gtatcccctt ttt Asn Asn Val Ser Val Ser 115 Leu Al a Leu Val 190 Gy Asn Val t ca Ser ct g Leu gca Al a gtt Val ct g Leu tgg Tr p aac Asn 110 Ar g Val Tyr 175 Gy Phe bet Ser aac Asn gat Asp gag G u gtc Val aag Lys gca Al a acc Thr Page 426 seql i st cor r ect ed. t xt <210> 358 <211> 118 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 358 G y Val Leu Pro Asp Trp Met Ser Thr 1 5 G y Val Leu Arg y u His I I e Pro 25 Ser Asp Ala Val Leu Pro Asn y Asn 40 Asp Lys Leu Tyr Ala Asp Leu Asn Pro Cys G n Val G y Asp Arg Ala Ala Hi s 70 Leu Leu y Phe Asn Asn Val Arg Asn Trp G y Asn IVet Val Arg IVet Pro lie 100 105 Asn Asn Val Ser Val Ser 115 <210> 359 <211> 3945 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(3922) <223> RXN01499 <400> 359 gcagcaatta tctccaccga agaggactaa ata acagttcctg agatccgccc aggcgccccc gaa Al a 10 Gy Phe Al a Thr Tyr 90 G u Thr Al a Ar g Asp Tr p Asp Thr Pr o Asn Ar g Thr Val Ser G u Ser Leu Al a Val Leu Tr p Asn 110 Asn Asp G u Val Lys Al a Thr gaa G u cag G n gat Asp cgc Ar g ct c Leu gt c Val ttg Leu at t SIle ccg Pr o gct Al a tct Ser t ac Tyr gca Al a ggt Sy gct Al a act Thr gca Al a cag G n acg Thr gct Al a 75 t at Tyr gag G u ggc G y cgt Ar g t ac Tyr gag G u at g Met cgc Ar g ctt Leu cca Pr o t aacgtg gcattgagca gtgttccagc igcgtact ttg tgg gac gtc tta Leu Trp Asp Val Leu 1 gca gca gct att gac gat ggc Ala Ala Ala lie Asp Asp G y 15 gaa gaa gtc acc gcg ttg gct G u G u Val Thr Ala Leu Ala cgt ggt gat cgc atc ggt att Arg Gy Asp Arg lie Gy lie tac atc gct att ttg gcc act Tyr Ile Ala lie Leu Ala Thr gtt gat gca gat gat cct gaa Val Asp Ala Asp Asp Pro G u 80 Page 427 seql i st cor r ect ed. t xt gag cgc gcc gag G u Arg Ala G u gac Asp cgt Ar g t cc Ser gct Al a 150 ggc G y ttt Phe tgc Cys ggc Gy act Thr 230 ct c Leu tcg Ser acg Thr at t SIle ggc Gy 310 gt g Val gcg Al a acc Thr cca Pr o 120 ggc G y gt g Val ctt Leu gca Al a gt g Val 200 tgg Tr p gct Al a gt c Val cct Pr o gtt Val 280 ttg Leu cct Pr o ctt Leu ct g Leu at g Met ttc Phe ttg Leu cct Pr o gcc Al a cca Pr o 170 tgt Cys gca Al a at t SIle ct g Leu ggc Gy 250 cgc Ar g tgt Cys ctt Leu gga Gy cgc Ar g 330 tct Ser gtg ttt ggt gaa Val Phe Gy G u cat Hi s gat Asp aag Lys gaa G u 155 gaa G u gag G u cca Pr o cgc Ar g tgg Tr p 235 gag G u gag G u ggc G y gct Al a at c SIle 315 tac Tyr gtt Val gca at g Met gat Asp ggt G y 140 gca Al a gac Asp gaa G u cgc Ar g cgc Ar g 220 cca Pr o gct Al a gt g Val act Thr ggt G y 300 ggc Gy ctt Leu ggt Gy ctt Leu acg Thr 125 gt g Val caa G n cga Ar g at g Met t cc Ser 205 gac Asp gca Al a tgc Cys tgg Tr p caa G n 285 tgg Tr p gag Gu gat Asp tgg Tr p cgc Ar g 110 gcg Al a gct Al a at g Met gt c Val tgg Tr p 190 ct a Leu at c SIle gaa G u tcg Ser aac Asn 270 ct c Leu gat Asp gt c Val cca Pr o acc Thr 350 AI a 95 ccg Pr o tgg Tr p gt g Val ttc Phe ctt Leu 175 ttg Leu gt c Val agt Ser gca Al a cag G n 255 act Thr t at Tyr ctt Leu ggc Gy gaa G u 335 cgc Ar g aat Asn acc Thr at t SIle t cc Ser ctt Leu 160 gcg Al a gcc Al a cgt Ar g gt c Val ttg Leu 240 gag G u t ac Tyr gct Al a gtt Val gaa Gu 320 aaa Lys gct Al a att aat gcg lI e Asn Al a gcg ggc ggc Al a G y G y 115 atc ttt act Ile Phe Thr 130 cac cgt t ca His Arg Ser 145 gt c gat cac Val Asp Hi s gga ttg tct G y Leu Ser tgg ggc cac Trp G y Hi s 195 tcc ggt atg Ser G y Vbet 210 gtc tcc acc Val Ser Thr 225 t ca cag gt c Ser G n Val ctc gtt gaa Leu Val G u ggc ccc acc G y Pro Thr 275 ggt cag cca G y G n Pr o 290 gtt gtc gac Val Val Asp 305 ttg gtc atc Leu Val I le gac cgc gag Asp Arg u tat cgt t cc Tyr Arg Ser 355 ctt Leu 100 gat Asp t cc Ser gct Al a cct Pr o gt a Val 180 ggc Gy gac Asp gt c Val cgc Ar g cgc Ar g 260 gaa G u gt g Val gat Asp ggt Gy aag Lys 340 ggt Gy ttc Phe acc Thr ggt G y gcg Al a t cc Ser 165 gcc Al a gcc Al a ttg Leu cca Pr o ttg Leu 245 tta Leu gca Al a ggc G y gcc Al a ggt Gy 325 t at Tyr gac Asp 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 Page 428 seql i st cor r ect ed. t xt cac Hi s gat Asp gcc Al a 390 cag G n gaa G u acc Thr gat Asp tgg Tr p 470 gaa G u agc Ser gct Al a gt g Val gt c Val 550 cag G n at g Met tgg Tr p ttc Phe cgt Ar g 360 gt g Val gt g Val act Thr gct Al a acc Thr 440 cct Pr o ctt Leu tgg Tr p aaa Lys act Thr 520 gat Asp gct Al a gag G u acc Thr gca Al a 600 tgg Tr p ctg gaa gaa Leu G u G u at c SIle gcg Al a gcg Al a 410 gct Al a cct Pr o acc Thr ggc Gy gct Al a 490 gcc Al a gtt Val t ac Tyr gcc Al a cgc Ar g 570 at c SIle ttg Leu gtt Val ggc G y ctt Leu 395 gat Asp gga G y gct Al a acc Thr acc Thr 475 cag G n gac Asp ggc Gy gat Asp gcc Al a 555 gt c Val cag G n ct g Leu cat Hi s gat Asp ggt G y 380 t cc Ser caa G n ttt Phe gct Al a t cc Ser 460 gt g Val gaa G u ttc Phe cgg Ar g cac Hi s 540 gac Asp gt c Val at t SIle ttg Leu gt c Val 620 ggc G y 365 cga Ar g aac Asn aaa Lys gat Asp ttg Leu 445 ggc G y gt g Val tgg Tr p ttc Phe gt a Val 525 cct Pr o act Thr aag Lys ccg Pr o ggc G y 605 t cc Ser ctc tac ttt gtg ggc Leu Tyr Phe Val G y 370 cgc at c gag ctc ggt Arg lie u Leu y 385 gtt cgt tcc tcc gca Val Arg Ser Ser Ala 400 gtt ctg gtt gca tac Val Leu Val Ala Tyr 415 cac aac gtc gcg act His Asn Val Ala Thr 430 gtt ccg cgc att cac Val Pro Arg Ile His 450 aag gtt gat aag aag Lys Val Asp Lys Lys 465 gaa gct aat gac ct c G u Ala Asn Asp Leu 480 gtc gat atc ctc ggc Val Asp Ile Leu y 495 tcc ctt ggc ggt acc Ser Leu y y Thr 510 cgc gca aag gtt ccc Arg Ala Lys Val Pr o 530 cgc ttg gag aaa ttc Arg Leu G u Lys Phe 545 ggc att tct ttg gag Sy Ile Ser Leu u 560 cct gtt tct ttt ggc Pro Val Ser Phe y 575 atc atg acg ctg caa Ile IVet Thr Leu n 590 aac aac atc atg gca Asn Asn Ile IVet Ala 610 tgg tgg ctt gtc atc Trp Trp Leu Val lie cgc Ar g gaa G u gt g Val gtt Val gcc Al a 435 gt g Val tct Ser agc Ser act Thr tct Ser 515 acc Thr gcc Al a gcg Al a act Thr gca Al a 595 gcg Al a gtt Val gtt Val gtt Val tct Ser 420 cga Ar g at g Met ttg Leu gca Al a tct Ser 500 ct c Leu gct Al a gag G u cca Pr o cgt Ar g 580 gca Al a ctt Leu 1219 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1987 ggc atg att G y Iet lie Page 429 seql i st cor r ect ed. t xt ttg Leu 630 gcc Al a ggt Sy tct Ser cgt Ar g cca Pr o 710 caa G n gt a Val acc Thr ggt Gy gct Al a 790 acc Thr acc Thr gct Al a tgg Tr p gct Al a 870 ttc gct acc Phe Al a Thr at c l Ie act Thr tct Ser 680 ct g Leu at c SIle gtt Val acc Thr ctt Leu 760 aca Thr t cc Ser cat Hi s at c SIle at c l Ie 840 gt g Val acc Thr acc Thr 650 ct g Leu aat Asn gt g Val ggc G y ctt Leu 730 gag Gu ggc Gy act Thr gca Al a cca Pr o 810 ttg Leu ttg Leu gtt Val acc Thr ccg att gc Pro Ile G 635 cgt ggc at Arg G y II cgc att tc Ar g II e Tr att tct gc II e Ser G 6E aag at g gc Lys bt G 700 ctt ttg ac Leu Leu TI 715 cgt ggc t Ar g G y T) gtc cat ge Val His As acc gtg gt Thr Val VE 7E ggt gat aE G y Asp L) 780 caa aag gt G n Lys VE 795 cgc agg tc Arg Arg SE tcg ctg ct Ser Leu Le tgg ctg gc Trp Leu Al 8z ttt gct ac Phe Al a TI 860 atc tgg gt lie Trp VE 875 y Arg Leu cgc ttg ccg act Thr t cc Ser 670 gca Al a aag Lys ttg Leu tgg Tr p aac Asn 750 ggc Gy acc Thr ggt Gy cgg Ar g cca Pr o 830 acg Thr gt c Val ctt Leu cct Pr o 655 gcc Al a acc Thr ggc G y ggc G y ct c Leu 735 gct Al a acc Thr at c SIle cgt Ar g tgg Tr p 815 ctt Leu at t SIle ggc G y gt c Val t cc Ser 895 Pr o 640 ggc G y gag G u tgg Tr p gt g Val aac Asn 720 gac Asp cgc Ar g ggc Gy aag Lys gca Al a 800 gtt Val cag G n agc Ser gcg Al a cgt Ar g 880 cgt Ar g atc ggc ggt lie y G y t cc t ac cct Ser Tyr Pro cgc ctt gct Arg Leu Ala 675 gt g aac t ac Val Asn Tyr 690 gat ctt cac Asp Leu Hi s 705 aat gtt tcc Asn Val Ser ggc gat at c G y Asp Ile at c ggc gct i e G y Al a 755 gct cac ct g Al a Hi s Leu 770 cct ggt t ct Pro G y Ser 785 aag cac cgg Lys His Ar g ccg gtg ttc Pro Val Phe gct ct c gct Al a Leu Al a 835 ccg ctt cca Pro Leu Pro 850 ttg gct gcg Leu Al a Al a 865 ttg atc cag Leu Ile G n ctt ggt tgg Leu G y Trp tgg Tr p cgt Ar g 660 gat Asp ttc Phe t cc Ser at c SIle ct g Leu 740 cgt Ar g ct g Leu cgt Ar g ttc Phe ggc G y 820 at t l Ie ct g Leu ttc Phe at c SIle cag G n 900 ggc Gy 645 ggc G y gcc Al a gcg Al a ct g Leu gag G u 725 cgt Ar g t cc Ser cct Pr o tgg Tr p cca Pr o 805 gcg Al a ggc G y at c SIe ttt Phe ggc G y 885 gt c Val 2035 2083 2131 2179 2227 2275 2323 2371 2419 2467 2515 2563 2611 2659 2707 2755 2803 atc aag ggc ggc acc gca cca gtg agg Ile Lys Gy y Thr Ala Pro Val Arg 890 Page 430 t gg Tr p ct c Le u aag Ly s ct g Le u 950 ggt G31y gt g ValI gt t ValI gca Al a 920 ggc G31y g at As p t at Tyr aag Lys cgc Ar g c aa O31n g at As p cgc Ar g ct g Le u 970 t cc Ser ct c Le u ct g Le u gt t ValI gaa G31u 955 ggt G31y t t c Ph e at g vb t ac c Thr gag G31u 940 ggc G31y aat As n at t I Ie seql i st car r ect ed. t xt gac gat gcc cgc acc t at Asp Asp Al a Ar g Th r Tyr 910 cca ct g t gg t tc cgc agc Pr o Leu Trp Phe Ar g Ser 925 930 at c t cc acc gcg gt g at g Ilie Ser Thr Al a Val I'b t 945 gca t tc ct g gcc gat gac Al a Phe Leu Al a Asp Asp 960 ggt t gg ct g ct c agt ggt G31y Tr p Leu Leu Ser (31y 975 ggt aac t ct ggc at c gca 03 y Asn Ser 03 y Il e Al a 990 ct c Leu 915 t tg Le u gt t ValI ac c Thr g aa G31u gga G31y 995 tt c Ph e ggc G31y cct Pr a ct c Le u ac c Thr 980 cct Pr a 2851 2899 2947 2995 3043 3091 cgc Ar gI aag LysI at g vb t 1 030 agc SerI cgt Ar gI ct g LeuI tt g LeuI at t I Ie 1110 gag 31uI gcg t Al aI aag Lys aag Lys 1015 cgt Ar g cct Pr a ct g e u ct c e u ct t e u 1 095 ac g Thr cac HIi s t c Phe ot c Le u 1000C gct Ala cgt Ar g ct t Le u at g vb t 1 080 (31y gtt ValI cct Pr a gt g ValI gct Al a aag Lys gt c IVal Phe gct Al a 1 065 c ag O31n gga (31y gt g ValI ct c t LeuF g aa O31u 1145 aag Ly s gcc Al a act Thr jgt 050 cca Pr a t ac Tyr ct g Le u at g vb t t c Phe 1130 t cc Ser aact As n aact As n gt c ValI 1 035 gt g Val at a I e- ct g LeuI at c I I aag Lys 1115 agc Ser gt g Val cc Se r cc Se r 020 g aa O31L aag Lys ac c Fh r ct c e u ct c e u t gg Tr p cgc Nr g gct NI a ct g Le u 1 005 aac As n gt t Val tt t Phe t ct Ser act Thr 1 085 at g vb t gt t Val t t t Phe ggc G31y 1165 gt t ValI t gg Tr p g at As p gca NI a ggt 1 07 g ag ac C Thr t gc Cys gt g a I cca Pr a 150 gca Al a t gg Tr p gag )31u cgt Ar g 1 055 gt g ValI tt c Phe gt t ValI gt c Val t gg Tr p 1135 t gg Tr p gt g ct c t Val Leu ggt t cc O3 y Ser 1 025 ggc gaa G31y O31u 1 040 ggc gcg O3 y Al a t tg gct Leu Al a aac at g t Asn Nib t- ggt gt g O3 y Val 1105 ggc aag G31y Lys 1120 ct g aat Leu Asn t tc ct c Phe Leu cc Se r 1010C cct Pr c gcE Ale gt g ValI gcg Al a gg Tr p 1 090 ct c Le L cat Hi s gag 31u gt g ValI 3y 11 t cc Ser cca Pr o aag Lys gaa O31u ct g Le u 1 075 at c I Ie gcc Al a aag Lys ct g Leu ccc Pr a 1155 ac c Thr gag O31u ac c Thr ac c Th r 1 060 t ca Ser ac c Thr at g vb t ccg Pr a caa 31n 1140 aac As n ccg Pr a cgc Ar g t ac Tyr 1 045 gca Al a ct g Leu t gg Tr p ggc t ct Ser 1125 g at As p ct g Leu 3139 3187 3235 3283 3331 3379 3427 3475 3523 3571 3619 ggc acc ggc 03 y Th r 03 y 1160 gcg ct g aac gcc Al a Leu Asn Al a at g agc gcg ct t N/t Ser Al a Leu gca cac at c Al a Hi s I I e Page 431 seqI i st car r ect ed. t xt ggc cgt ggc gca tgg atc gaa tcc tac tgg ctg ccg G31y Ar g G31y Ala Tr p I Ie (31u Ser Tyr Tr p Leu Pro 1175 1180 1185 tgc tac atc ggc aag ggc gca acc gtg ggc cct ggc Cys Tyr I Ie G31y Lys (31y Ala Thr Va 1G31y Pr o G31y 1190 1195 1200 acc cac ct c t tc cag gac cgc gtg at g agc ct a gat Thr Hi s Leu Phe O31n Asp Ar g Val I'b t Ser Leu Asp- 1210 1215 gct gac ggc gcc acc cta gcg gac cac tcc gtt gcc Ala Asp (1y Ala Thr Leu AlaAsp Hi s Ser Val Ala 1225 1230 ctt atc gac gcc tcc gcc acc atc ggc cca ggc tcg LeulIle Asp Ala Ser AlaThr Ilie 31y Pr o 1y Seri 1240 1245 ggc gac aag gta cca gcg cat acc cgc tgg caa ggc 31y Asp Lys Val Pr oAla Hi s Thr Ar gTr p (1n (1y 1255 1260 1265 ccg t gg agc aac t ct t aaat aacaa caat cagccg gat Pr o Tr p Ser Asn Ser 1270 <210> 360 <211> 1274 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn g ae gt g ValI ac g Vh r ct t e u ct g e u 1250 aac Asrn acc Thr gt c ValI gt g Val- cct Pr o 1235 gt g ValI cca Pr o g ac As p gt g ValI ac c Vh r 1220 gct A a at g vb t at t I Ie ct c Leu c ag 1 205 gt c ValI t cg Ser cgc Ar g gag 3667 3715 3763 3811 3859 3907 3945 Le u A a ValI As p A a A a I Ie A a I Ie Hi s 145 ValI 360 Tr p Asp Val I I e Asp Asr 2C Thr A a LeL Ar g I Ie (31 1 1 e Leu A E As p As p Pr c As n A a LeL 1 OC (31y 1y As r 115 Phe Thr Ser 130 Ar g Ser AlE As i Hi s Pr c Leu 03 u Ser 03 y 03 n Val A a As p Ser I Ie Ar g I'b t 55 Thr Leu A a 70 (31u (31u Ar g Phe As p A a Thr Ar g Ar g 03 y Ser Thr 135 A a A a Phe 150 Ser (3y Pr o 1 65 ValI A a Se r 10 Leu Thr Ty r 25 1I e Hi s A a 40 Pr o Ser G31y A a 03 y A a A a 03 u [Vb t 90 Thr 03 y Phe 1 05 Pr o Ar g Leu 120 G31y Lys Pr o Val As p Ala Leu (3y Pr o Thr A a O31n Thr A a 75 ValI Hi s As p Lys5 O31u 1 55 Tyr O31u G31y Ar g Tyr Ph e vb t As p G31y 140 A a Pr o Le u I Ie As p ValI G31y Le u Thr 125 ValI O31n (31u vb t Ar g Le u Pr o (31u Ar g 110 A a A a vb t A a (31u Ar g Tyr ValI A a Pr a Tr p ValI Ph e A a (31u Gy le As p As n Thr I Ie Se r Le u 1 cOu Asp Arg Val Leu A a 1 Page 432 seqi i st car r ect ed. t xt G31y Leu Ser Val Al a Phe Asp Al a Ser Cy s (1u 1u lb t Trp Leu Ala 1 1 80 Tr p Ser ValI 225 Ser Le u G31y G31y ValI 305 Le u As p Tyr ValI Le u 385 Ser Al a Al a I Ie Ly s 465 As p Le u G31y Hi s 1 95 vb t Thr ValI O31u Thr 275 Pr o As p I Ie O31u Ser 355 Ar g O31u ValI ValI Al a 435 ValI Ser Ser Thr Ser 515 Al a Le u Pr o Le u 245 Le u Al a G31y Al a G31y 325 Tyr As p As p As p ValI 405 Le u Le u As p Pr o Thr 485 ValI Al a Le u Pr o 215 Le u I Ie Thr ValI G31y 295 (31u G31y Pr a ValI O31n 375 As n Thr As p O31u Le u 455 Pr a Al a Ser Al a 1 85 Pr a Le u G31y G31y As p 265 Al a Pr a ValI Al a Ly s 345 Le u Ly s Al a G31y Al a 425 vb t ValI Pr a I Ie As p 505 Le u Al a I Ie Le u G31y 250 Ar g cys Le u (31y Ar g 330 Ser O31u I Ie Al a Al a 410 Al a Pr a Thr G31y Al a 490 Al a ValI Pr a Ar g Tr p 235 O31u O31u G31y Al a I Ie 315 Tyr ValI O31u G31y Le u 395 As p G31y Al a Thr Thr 475 O31n As p G31y Ar g Ar g 220 Pr a Al a ValI Thr G31y 300 G31y Le u G31y As p G31y 380 Ser O31n Ph e Al a Ser 460 ValI O31u Ph e Ar g Ser 205 As p Al a cys Tr p O31n 285 Tr p (31u As p Tr p O31y 365 Ar g As n Ly s As p Le u 445 O31y ValI Tr p Ph e ValI 525 ValI Ser Al a O31n 255 Thr Tyr Le u G31y O31u 335 Ar g Tyr I Ie Ar g Le u 415 As n Pr a ValI Al a As p 495 Le u Al a Ar g ValI Le u 240 O31u Tyr Al a ValI (31u 320 Ly s Al a Ph e O31u Ser 400 ValI ValI Ar g As p As n 480 li1e O31y Ly s Val Pro Thr Ala Ala Val Arg Asp Leu Tyr Asp His Pro Arg Leu Ou Page 433 seqi i st car r ect ed. t xt 535 0 u Al a IIe Al a Ly s 545 Le u Ph e Le u vb t ValI 625 li1e Ser Ar g ValI As p 705 As n G31y I Ie Al a Pr o 785 Ly s Pr o Al a Pr o Le u 865 Le u Al a Al a Thr Al a 595 Al a G31y G31y Pr o Al a 675 Tyr Hi s Ser I Ie Al a 755 Le u Ser Ar g Ph e Al a 835 Pr a Al a O31n Ar g As n 565 ValI O31n As p I Ie G31y 645 G31y Al a Al a Le u O31u 725 Ar g Ser Pr a Tr p Pr a 805 Al a G31y I Ie Ph e G31y 885 ValI 550 (31n vb t Tr p Ph e Le u 630 Al a G31y Ser Ar g Pr a 71 0 O31n ValI Thr G31y Al a 790 Thr Thr Al a Tr p Al a 870 li1e ValI O31n I Ie As p 615 ValI Ar g Ser O31y Ser 695 Pr a O31u O31y Le u Ser 775 O31y Ser Ser Al a O31y 855 Tyr Ly s (31u Le u 585 Tr p Al a Al a I Ie Hi s 665 Ar g O31y Thr As p I Ie 745 Pr a ValI Pr a Pr a ValI 825 Thr Le u ValI (31y Ar g 570 li1e Le u ValI Thr Thr 650 Le u As n ValI O31y Le u 730 O31u O31y Thr Al a Pr a 810 Le u Le u ValI Thr Thr 890 Al a As p 555 Val Val O31n I Ie Leu Leu Hi s Val 620 Pr o I Ie 635 Ar g O31y Ar g I Ie I I e Ser Lys l'b t 700 Leu Leu 715 Ar g O31y Val Hi s Thr Val O31yAs p 780 O3 n Lys 795 Ar g Ar g Ser Leu Trp Leu Phe Al a 860 li1e Tr p 875 Al a Pr o Th r 03 y I I e Ly s Pr a O31y 605 Ser O31y I Ie Tr p O31y 685 O31y Thr Tyr As p ValI 765 Lys5 ValI Ser Le u Al a 845 Thr ValI ValI ValI 575 vb t As n Tr p Le u Pr a 655 Al a Thr O31y O31y Le u 735 Al a Thr I Ie Ar g Tr p 815 Le u I Ie O31y ValI Ser 895 Ser 560 Ser Thr I Ie Le u Pr a 640 O31y O31u Tr p ValI As n 720 As p Ar g O31y Al a 800 ValI O31n Se r Al a Ar g 880 Ar g Page 434 Le u Thr Ar g ValI 945 As p Ser I Ie Le u Ser 1 025 (31u Al a Al a Nibt ValI 11 05 Ly s As n Le u Le u Pr o 11 85 G31y As p Al a G31y Tr p O31n 900 Ty r Leu Phe 915 Ser Leu G31y 930 I'b t Val Pr o As p Thr Leu 03 y 03 u Thr 980 Ala G31y Pr o 995 Ser Ser Thr 1010 Pr o Pr o (31L Al a Lys Thr Val (31u Th r 1 06C Al a Leu Ser 1 075 Tr p I Ie Thr 1 090 Leu Al a I'bt Hi s Lys Pro 03 u Leu (3 n 114C Val Pr o Asrn 1155 G31y Al a Hi s 1170 03 u Th r As r Val Val Val Thr Val Thr 122C Leu Pro Al E 1235 I la I Pr o %I a Lys Ile Nr g Al a Tyr I Ie 935 Al a G31y G31y Lys5 seqI i st cor r ect ed. t xt Val O31n Ar g Leu I'bt Asp Asp Al a 905 910 Al a Ser O31n Leu Thr Pr o Leu Trp 920 925 G31y Lys As p Val O31u I Ie Ser Thr 940 As p I Ie Ar g 31u G31y Al a Phe Leu 955 Tyr O31u Leu G31y As n G31y Tr p Leu 970 975 Lys Ar g Ser Phe I Ie G31y As n Ser 985 990 Leu Al a Lys Asn Ser Leu Val Al Ar g Ph e Al a Al a 960 Le u G31y a Val 1 000 1 005 Pr o Lys Lys Al a Lys 1015 Ar g IVb t Ar g Ar g Val 1 030 Tyr Ser Pro G31y Ph e 1 045 Ala Arg Leu Leu Ala 1065 Leu Leu Leu I'bt 03 n 1 080 Tr p Leu Leu G31y G31y 1 095 G31y I Ie Thr Val Val 1110 Ser G31u Hi s Pro Leu 1125 Asp Al a Phe Val O31u 1145 1Leu G31y Thr G31y Al a 1160 Ilie G31y Ar g G31y Al a 1175 Leu Cys Tyr I Ie G31y 1190 O31n Thr His Leu Phe 1205 Val Ala Asp Gy Ala 1225 3Ser Leu Ilie Asp Ala 1240 Al a As n Se r As n Tr p Tr p (31y Thr 1 050 Pr o Tyr Le u vb t Ph e 1130 Ser Le u Tr p Lys5 1210 Thr Ser ValI 1 035 ValI I Ie Le u I Ie Lys5 11 15 Ser ValI As n I Ie G31y 11 95 As p Le u Al a 1 020 31u ValI As p Lys Phe Al a Thr Se r 03 y 1 070 Leu Thr O31u 1 085 Leu I'bt Thr 1100 Tr p Val Cys Ar g Phe Val- Ala G31y Pr o 1150 Al a 1y [ib t 1165 O31u Ser Tyr 1180 Al a Thr Val Ar g Val I 'bt Al a As p Hi s 1230 Thr I Ie G31y 1245 (31u Ar g 1 055 ValI Ph e ValI ValI Tr p 1135 Tr p Ser Tr p G31y Se r 1215 Ser Pr o 1 040 Leu As n 1120 Leu Ph e Al a Leu Pr o 1 200 Leu ValI Ser Leu Val N/t Arg cy 1250 As p 1255 Lys Val Pro Ala His Th r Ar g Tr p Q n 1260 Page 435 seql i st cor r ect ed. t xt Sy Asn Pro Ile u Pro Trp Ser Asn Ser 1265 1270 <210> 361 <211> 609 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(586) <223> RXN01997 <400> 361 aaaaaggtgg gaaacttagc caatccaaag cccaaaaatg cgggttatgc tgcgctaacc tatgctgaca gccttgcgga agt t gtgtac gttaggggcc aag Lys t ca Ser acc Thr cac Hi s cag G n gag G u at c SIle cgc Ar g acc Thr t cc Ser 150 gca Al a gt g Val at g Met cag G n ggt Sy ttc Phe gag G u ct g Leu 120 ggc Sy at c SIle gct Al a ctt Leu gac Asp ttc Phe at t SIle gct Al a gct Al a gac Asp at c SIle tt c Phe cag G n tgg Tr p gct Al a gca Al a 75 t cc Ser gcc Al a tgg Tr p at c SIle aac Asn ct c Leu at g Met cag G n cca Pr o ct g Leu at c l Ie ttc Phe gac Asp 140 aac Asn t cc Ser aag Lys 45 cac Hi s cca Pr o gca Al a cag G n ct c Leu 125 cgc Ar g caa G n t ac Tyr gca Al a ctt Leu aag Lys cac Hi s gac Asp 110 aac Asn ct c Leu gt g Val 15 gtt Val cag G n ctt Leu ctt Leu gag G u 95 gct Al a gag G u cgt Ar g act Thr ct a Leu agc Ser gac Asp gat Asp 80 cag G n gag G u cag G n at c SIle at g Met 1 gca Al a gac Asp aaa Lys cgt Ar g gt c Val aag Lys aag Lys at c SIle gct Al a 145 aca atc aac gag Thr Ile Asn u gag ctt gaa gct G u Leu G u Al a gat ctg ggc ctc Asp Leu G y Leu gaa gag ctc gaa G u G u Leu G u gac tac acc cca Asp Tyr Thr Pro acc tcc gct atc Thr Ser Ala lle atc tcc ggc ctg I e Ser G y Leu 100 gac tac gat tcc Asp Tyr Asp Ser 115 gaa gaa gaa gca Su u u Ala 130 ggt gat tcc ggt G y Asp Ser G y cgc at c Arg Ile 155 gac ggc gaa ctc Asp G y G u Leu ggc tcc cgc G y Ser Arg 160 t aaat t cccc gcagttttta atg <210> 362 <211> 162 <212> PRT Page 436 sec <213> Cor ynebact eri um gl ut am cum <400> 362 IVet Thr Ile Asn u Lys lie Ala S Si st cor r ect ed. t xt ier 1 Al a Asp Lys Ar g Val Lys Lys SIle Al a 145 Ser Leu Leu G u Tyr Ser Ser Tyr 115 G u 5 Al a Leu G u Pr o SIle Leu Ser Al a Mbet Gy Al a I le Al a Ar g Al a Val 135 Tyr Ar g Lys Asp G u Leu 105 l le G u Al a 10 Leu Asp Phe SIle Al a 90 Al a Asp SIle Phe G n Tr p Al a Al a Ser Al a Tr p SIle Asn Ser Lys Hi s Pr o Al a G n Leu 125 Ar g G n Tyr Al a Leu Lys Hi s Asp 110 Asn Leu Val Val G n Leu Leu G u Al a G u Ar g Asp Ser G y Gy Ile Leu Arg Asp G y G u Leu <210> 363 <211> 867 <212> DNA <213> Cor ynebact eri um gl ut arri cum <220> <221> CDS <222> (101)..(844) <223> RXA01848 <400> 363 ctgcaaggaa ctgcgcaggc gaaggcgcag act ggcgacccct ttcgctccta ggcatttgcg cct act ggaa aggtaggtac tgccggatcc ggcgtcc atg ggg gag gag gac Vb t Gy Gu u Asp 1 tat tcg cgc cag ggc gct gat Tyr Ser Arg G n Gy Ala Asp 15 atc aac tta gtg ggc acg ctc Ile Asn Leu Val y Thr Leu att gtt gaa ggc gtg gtc gat Ile Val G u y Val Val Asp ctg cgc cgt ggg cgc gaa gcc Leu Arg Arg G y Arg G u Ala Page 437 t cc Ser gt c Val at g Met ttc Phe cca Pr o ccc Pr o gct Al a ccc Pr o agg Ar g aag Lys gaa G u gac Asp t cc Ser gga Gy ggc Gy cgg Ar g aag Lys cac Hi s gcc Al a 45 ggt Gy gcg Al a ggc Gy aac Asn tcg Ser IND seqI i st car r ect ed. t xt 60 aac ct c at c gt g t tc gt c gt c gac aca tcg ggg t cg at g gct gcg cgt 355 Asn Leu I Ie Val Phe Val Val Asp Thr Ser G31y Ser I'b t Ala Ala Ar g fl 70 75 80 t tcc agg gt g cgt gcg gt c acc ggg act at t acc t ct at g ct t aac gac 403 Ser Ar g Val Ar g Ala Val Thr G31y Thr Ilie Thr Ser I'b t Leu Asn Asp c~190 95 100 gcc tac cag cgc cgc gac aag gtt gcg gtt atc gcg gtc aac ggc aac 451 Ala Tyr O31n Ar g Ar g Asp Lys Val Ala Val Ilie Ala Val Asn G31y Asn c-1105 110 115 00 aag ccg aca ct g gt g t t g aat cca aca aat t ct gt g gag caa gct cag 499 SLys Pr oThr Leu Val Leu Asn Pr oThr Asn Ser Val (1u (1n Ala (1n 120 125 130 .C cag aaa t ta aag gat at g ccg at g ggt ggt cgc act cca ct g gca gag 547 (3 GnLys Leu Lys Asp b t Pr o b t (1y 1y Ar gThr Pr oLeu Ala (1u 135 140 145 ggg ctg ctc atg gcc aag gat ctc atg gca agg gaa ctc cga aag gaa 595 G31y Leu Leu I'b t Ala Lys Asp Leu I'b t Ala Ar g G31u Leu Ar g Lys G31u 150 155 160 165 ccc ggc cga cgc gcg atc c t c at g gt g at g acc gat ggc caa gac acc 643 Pro 1y Ar gAr g Aa Ilie Leu I'b t Val I'b t Thr Asp (1y (1n Asp Thr 170 175 180 tcc gat gcc ggc gaa gca ggc att gcc acc gcg gcg gaa aca gtg gtg 691 Ser Asp Ala (1y (1u Ala (1y Ile AlaThr Ala Ala 1u Thr Val Val 185 190 195 aaa tca cga ctg tcc ggc aac gtg gtc atc gac tgc gaa ggc cga ctc 739 Lys Ser Ar g Leu Ser G31y Asn Val Val Ilie Asp Cys G31u G31y Ar g Leu 200 205 210 aaa gtg cgc aaa gag cgc gcc ggg gtg ttg gct gaa atg ctc ggt ggt 787 Lys Val Ar g Lys G31u Ar g Ala G31y Val Leu Ala 31u I'b t Leu G31y G31y 215 220 225 gt g t gc gt g aga t tg cgt gat ct t aac t cc gag cac at c aaa at g gt g 835 Val Cys Val Ar g Leu Ar g Asp Leu Asn Ser G31u Hi s Ilie Lys I'b t Val 230 235 240 245 at t aac gcc t agacaacca gagt gagggt t tc 867 Il e Asn A a <210> 364 <211> 248 <212> PRT <213> Cor ynebact er i urn gi ut anm cumn <400> 364 'b t G31y Gu Gu Asp Ser Thr ProGy Arg Arg Ser Lys Ala Tyr Ser 1 5 10 Ar g(1n (1y Aa Asp Val Arg Pro l'bt Lys Gy Oy His Gy lleAsn 25 Leu Val G31y Thr Leu I'b t Ala Ala Th r G31u Arg Gy Ala Asn Ilie Val 40 (3u (3y Val Val Asp Phe Arg Pro Thr Asp Leu Ar g C3y Ser Leu Ar g 55 Page 438 seqI i st car r ect ed. t xt Ar g Ser Ser A a ValI Thr 145 O31u As p A a CYS O31u 225 Hi s G31y Ar g O31u Ala As n L eu I Ie ValI Phe Val Val Asp Thr Ser A a Le u As n 115 O31n Le u Ar g O31n Thr 1 95 G31y Le u Ly s Ar g As p As n O31n O31u (31u 1 65 Thr ValI Le u G31y ValI 245 Ser A a Ly s O31n O31y 150 Pr o Ser Ly s Ly s ValI 230 Ar g Tyr Pr o Ly s 135 Le u O31y As p Ser ValI 215 Cys Ar g Ar g 1 05 Le u Ly s vb t Ar g O31y 1 85 Le u Ly s Ar g A a 90 Ar g ValI As p A a A a 1 70 O31u Ser O31u Le u ValI As p Le u vb t Ly s 1 li1e A a G y Ar g Ar g 235 Thr Ly s As n Pr o 140 As p Le u O31y As n A a 220 As p O31y ValI Pr o 125 vb t Le u vb t I Ie ValI 205 O31y Le u Thr A a 110 Thr O31y vb t ValI A a 1 ValI ValI As n lIe ValI As n O31y A a vb t 1 Thr I Ie Le u Ser I I e As n A a <210O> 365 <21 1> 1224 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 CIDS <222> (1201) <223> RXN01849 <400> 365 aaaacct t aa gt t gggt ggt t aaacccact aaggt ct cac caccaaaaaa tctcaagaaa actcacatta aaggacagta at c I Ie gcg A a cga Ar g ggt t at Ty r at c I Ie gag O31u tt a tt c Ph e act Th r ggt O31y g at t cc Ser gcg A a aca Th r gcc gt t ValI t cc Ser aaa Ly s 45 t tg gt g ValI cca Pr a act Th r gt g gga caa (31y (1n 15 cgc att Ar g I Ie acc act Thr Thr aac tt g Page 43 t t tat ggat g t gccaggt ca at g gcg t ca caa cag I'b t A a Se r 03 n 03 n 1 gac gag ctt cgg ctt Asp O31u Leu Arg Leu ggt ggc gt g gt g at t O31y O31y Val Val Ilie gt g cgt gct t tt gct Val Ar g Ala Phe Ala cct ct C gga t cc acg seqi i st car r ect ed. t xt G31y Leu Leu G31y Asp Ala Pro Leu Val Asn Leu Pro Leu 60 03 y Ser Thr gag O31u ggc G31y gt g ValI gct A a ggt G31y aat As n 1 50 t tg Le u at c I Ie gct A a gct A a c ag O31n 230 g ac As p cgc Ar g tt g Le u gag O31u g ac As p 310 cgt Ar g gcg A a t at Tyr ct c Le u 120 t cg Ser gag O31u gt g ValI cgc Ar g t gg Tr p 200 g at As p gcg A a gt g ValI gt g ValI c ac Hi s 280 aag Ly s g aa O31u gt g ValI t at Tyr g at As p gca A a t ct Ser 99c G31y gt t ValI 1 70 c99 Ar g gag O31u ct g Le u tt g Le u ac g Thr 250 ac g Thr cgt Ar g c ag O31n aaa Ly s ggt G31y 75 cag O31n gag O31u gct A a t ca Ser gag O31u 155 gct A a ct t Le u caa O31n cct Pr o t gt Cy s 235 cgt Ar g gaa O31u cgc Ar g gaa O31u gga O31y 315 t cc Ser cca Pr o gt c ValI gca A a cca Pr o 140 ct g Le u gcg A a g at As p g at As p ggt O31y 220 gca A a ac c Thr g aa O31u cgt Ar g ac c Thr 300 cct Pr o ct c Le u ggt O31y aac As n ag c Ser 125 gca A a cgc Ar g t ct Ser tt t Ph e gcg A a 205 gt g ValI cgt Ar g gca A a g ac As p aat As n 285 ct g Le u gcc A a aac As n tt g Le u ct c Le u 110 ggt O31y aac As n ccg Pr a ac g Thr g aa O31u 1 g ac As p gag O31u at t I Ie ct t Le u gt g ValI 270 cct Pr a c ag O31n gcc A a at g vb t ct c Le u 95 tt g Le u cgc Ar g tt t Ph e c ag O31n aac As n 1 75 aac As n ac c Thr ct g Le u g aa O31u gct A a 255 gag O31u tt c Ph e g aa O31u aag Ly s g aa O31u 80 gcg A a gcg A a gt c ValI gt g ValI ct g Le u 1 60 cct Pr a gct A a t cc Ser ccg Pr a gt c ValI 240 c ac Hi s at c I Ie g at As p gct A a at c I Ie 320 act Thr c ag O31n g at As p ag c Ser tt g Le u 145 ct g Le u gag O31u cct Pr a aac As n g at As p 225 g ac As p gcc A a gca A a gct A a cgg Ar g 305 ac c Thr gt g ValI gct A a c ac Hi s at t I Ie 130 gt g ValI g ac As p gt g ValI gag O31u cgt Ar g 210 ct g Le u ggt O31y gcg A a gct A a cca Pr a 290 g ac As p g at As p ac c Thr ggc O31y 1 00 gt g ValI cgt Ar g ac c Thr tt c Ph e gt g ValI 1 tt c Ph e tt g Le u tt g Le u cgc Ar g gct A a 260 ct a Le u at g vb t tt c Ph e g aa (31u ac c Thr ggt O31y g at As p g ac As p at g vb t ggt O31y 1 gag O31u at g vb t gcg A a t cg Ser gct A a 245 gga O31y gcg A a gag O31u aaa Ly s ac c Thr 325 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1 027 1 075 1123 ggt gca gag gcc ttt acc gat acc gac aat ccc acc gag gaa gac ggt Page 440 seql i st cor r ect ed. t xt Sy Ala G u Ala Phe Thr Asp Thr Asp Asn Pro Thr G u G u Asp y 330 335 340 ctg caa gga act gcg cag gcg aag gcg cag act act gga aag gta ggt Leu G n y Thr Ala G n Ala Lys Ala G n Thr Thr y Lys Val G y 345 350 355 act gcc gga tcc ggc gac ccc ttt cgc tcc taggcatttg cgcctggcgt Thr Ala y Ser y Asp Pro Phe Arg Ser 360 365 cca <210> 366 <211> 367 <212> PRT <213> Cor ynebact eri um gl ut am cum 1171 1221 1224 <400> 366 Met Al a Ser 1 Asp G u Leu G y G y Val Val Arg Ala Pro Leu G y Thr Val Leu G n Al a Asp Asp His Leu 115 Ser Ile G u 130 Leu Val G y 145 Leu Asp Arg Su Val Arg Pro G u G n 195 Asn Arg lle 210 Asp Leu Ile 225 Asp G y IVet G n G n 5 Arg Leu Val lile Phe Al a Ser Thr Thr Thr G y G y 100 Val Asp Arg Asp Thr 'bet Phe G y 165 Val G u 180 Phe IVet Leu Al a Leu Ser Arg Ala 245 II e Arg Tyr Al a Ar g Sy G u 70 Sy Val Al a Sy Asn 150 Leu SIle Al a Al a G n 230 Asp Leu Sy Leu 55 Asp Ar g Leu Leu SIle 135 Pr o Al a SIle Lys Lys 215 I le Leu SIle G u 40 Leu Ar g Al a Tyr Leu 120 Ser G u Val Ar g Tr p 200 Asp Al a Val Pro Phe 10 Leu Thr Lys G y Sy Asp Val Val G u Tyr 90 Val Asp 105 Asp Al a Hi s Ser G u G y Asp Val 170 Arg Arg 185 Al a G u Leu Leu Trp Leu Ile Thr 250 Ser Al a Thr Al a Sy 75 G n G u Al a Ser G u 155 Al a Leu G n Pr o Cys 235 Al a l Ie Al a Pr o Ser Pr o Val Al a Pr o 140 Leu Al a Asp Asp Sy 220 Al a Val Ser Lys Leu Leu Sy Asn Ser 125 Al a Ar g Ser Phe Al a 205 Val Ar g Sy Ar g Thr Asn bet Leu Leu Ar g Phe G n Asn 175 Asn Thr Leu G u G n SIe Thr Leu G u Al a Al a Val Val Leu 160 Pr o Al a Ser Pr o Val 240 Arg Thr Ala Leu Ala His 255 Page 441 Al a Al a Al a Ar g 305 Thr Thr Thr Tr p Ar g 275 (31u Ph e (31u (31u Ly s 355 G31y Al a (31u Ly s Thr 325 G31y (31y Thr Pr o Ar g 295 As n Al a O31n Al a seql i st car r ect ed. t xt Val Val Thr O31u O31u Asp 265 Hi s Ar g Ar g Ar g Ar g As n 280 285 Lys Leu 03 n 03 u Thr Leu 300 O31u As p Lys G31y Pr o Al a 315 03 u Al a Phe Thr As p Thr 330 03 y Thr Al a 03 n Al a Lys 345 G31y Ser G31y As p Pr o Phe 360 365 ValI 270 Pr o O31n Al a As p Al a 350 Ar g O31u Ph e O31u As n 335 O31n Ser <210O> 367 <21 1> 473 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ari cumn <220> <221 CDS <222> (450) <223> FRXA01849 <400> 367 ct g cct ggt Leu Pro G31y ttg tgt gca Leu Cys Al a acg cgt acc Thr Ar g Thr acg gaa gaa Thr 03 u 03 u cgt cgc cgt Ar g Ar g Ar g cag gaa acc O3 n 03 u Thr aaa gga cct Lys G31y Pr o t tt acc gat Phe Thr Asp 115 gcg cag gcg Al a 03 n Al a 130 gag (31u at t I Ie ct t Le u gt g ValI cct Pr o c ag O31n gcc Al a g ac As p gcg Al a ct g Le u gaa O31u gct Al a gag O31u t t c Ph e 70 gaa O31u aag Lys5 aat As n cag (31n ccg Pr o gt c ValI c ac Hi s at c I Ie 55 g at As p gct Al a at c I Ie ccc Pr a act Thr 135 g at As p g ac As p gcc Al a 40 gca Al a gct Al a cgg Ar g ac c Thr ac c Thr 120 act Thr ct g Le u ggt O31y gcg Al a gct Al a cca Pr a g ac As p g at As p 1 05 gag O31u gga (31y at c lIe 10 at g vb t t gg Tr p cgc Ar g g aa O31u tt c Ph e 90 gag O31u g aa (31u tt g Le u cgc Ar g gct Al a ct a Le u at g vb t 75 tt c Ph e g aa O31u g ac As p t cg Ser gct Al a gga O31y gcg Al a gag O31u aaa Lys5 ac c Thr ggt (31y c ag O31n g ac As p cgc Ar g tt g Le u gag O31u g ac As p ggt O31y ct g Le u 125 at t I Ie ct g Le u act Thr ccg Pr a cgc Ar g aat As n gca Al a 110 c aa O31n gcg Al a gt g ValI gt g ValI c ac Hi s aag Lys5 g aa O31u gag O31u gga (31y t gg Tr p at c I Ie gt t ValI cgc Ar g ct t Le u g at As p gcc Al a act Thr aag gt a ggt Lys Val G31y 140 Page 442 act gcc gga t cc Thr Al a 03 y Ser ^0 seql i st cor r ect ed. t xt ggc gac ccc ttt cgc tcc taggcatttg cgcctggcgt cca 473 i G y Asp Pro Phe Arg Ser 145 150 <210> 368 <211> 150 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 368 CK1 Leu Pro Gy Val u Leu Pro Asp Leu Ile Leu Ser On Ile Ala Trp 1 5 10 00 S Leu Cys Ala Arg I le u Val Asp y IVet Arg Ala Asp Leu Val lie 25 \O Thr Arg Thr Ala Leu Ala His Ala Ala Trp Ala G y Arg Thr Val Val 40 Cq Thr G u G u Asp Val G u Ile Ala Ala Arg Leu Ala Leu Pro His Arg 55 Arg Arg Arg Asn Pro Phe Asp Ala Pro G u IVet u u Arg Lys Leu 70 75 Sn G u Thr Leu n G u Ala Arg Asp Phe Phe Lys Asp Asn G u Asp 90 Lys G y Pro Al a Al a Lys I e Thr Asp G u G u Thr G y Al a G u Al a 100 105 110 Phe Thr Asp Thr Asp Asn Pro Thr G u G u Asp y Leu G n y Thr 115 120 125 Ala G n Ala Lys Ala G n Thr Thr y Lys Val y Thr Ala y Ser 130 135 140 Sy Asp Pro Phe Arg Ser 145 150 <210> 369 <211> 667 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(667) <223> FRXA01691 <400> 369 aaaacct t aa gttgggtggt taaacccact aaggtctcac tttatggatg tgccaggtca caccaaaaaa tctcaagaaa actcacatta aaggacagta atg gcg tca caa cag 115 IVet Ala Ser n G n 1 atc cgc tat cca ttc tcc gcg gtt gtg gga caa gac gag ctt cgg ctt 163 lie Arg Tyr Pro Phe Ser Ala Val Val y G n Asp G u Leu Arg Leu 15 gcg ttg atc ctc act gcg att tcc cca cgc att ggt ggc gtg gtg att 211 Ala Leu Ile Leu Thr Ala lie Ser Pro Arg Ile y y Val Val lle 30 Page 443 seqI i st car r ect ed. t xt cga Ar g ggt G31y gag (31u ggc G31y gt g ValI gct Al a ggt (31y aat As n 1 50 t tg Le u at c I Ie ggt gag aag ggt aca gcg G y G u Lys y Thr Al a aaa act acc act gt g Thr Thr Val tt a Le u cgt Ar g gcg Al a t at Tyr ct c Le u 120 t cg Ser gag (31u gt g ValI cgc Ar g g at As p gt g ValI t at Tyr g at As p gca Al a t ct Ser 99c G31y gt t ValI 1 70 c99 Ar g gcc Al a ggt G31y 75 cag O31n gag O31u gct Al a t ca Ser gag O31u 155 gct Al a ct t Le u cct Pr o t cc Ser cca Pr o gt c ValI gca Al a cca Pr o 140 ct g Le u gcg Al a g at As p Ly s 45 tt g Le u ct c Le u ggt G31y aac As n ag c Ser 125 gca Al a cgc Ar g t ct Ser tt t Ph e gt g ValI aac As n tt g Le u ct c Le u 110 ggt G31y aac As n ccg Pr o ac g Thr tt g Le u g aa O31u gcg Al a gcg Al a gt c ValI gt g ValI ct g Le u 1 cct Pr o cgt gct Ar g Al a ct c gga Leu 03 y gt g tt g Val Leu gct gat Al a As p cac ct g Hi s Leu 115 at t gag I Ie O31u 130 gt g ggc Val G31y gac cgt Asp Arg gt g cgc Val Ar g tt t Ph e t cc Ser ac c Thr ggc G31y 1 00 gt g ValI cgt Ar g ac c Thr tt c Ph e gt g ValI 1 <210O> 370 <21 1> 189 <21 2> PRT <21 3> Cor ynebact er i urn gI ut ani cumn <400> 370 I'b t Al a Ser O31n O31n lieArg Tyr Pro 1 5 Asp O31u Leu Arg Leu Ala Leu Ilie Leu G31y G31y Val Val Ilie Ar g G31y 31u Lys 40 Val Ar g Al a Phe Al a G31y Leu Leu G31y 55 Pr o Leu G31y Ser Thr Ou Asp Arg Val 70 Thr Val Leu Thr Thr G31y Arg Ala O31u O31n Al a Asp G31y G31y Val Leu Tyr Val 100 105 Phe Ser Al a 10 Thr Al a I I e 03 y Thr Al a As p Al a Pr o Val G31y Ser Tyr O31n Pr o 90 As p 03 u Val Page 444 ValI Ser Ly s Le u Le u G31y As n G31y Ar g Th r As n vb t Le u Le u seqI i st car r ect ed. t xt Asp Hi s Leu Val Asp Ala Leu Leu Asp Ala Ala Ala Ser G31y Ar g Val 115 120 125 Ser I Ie O31u Ar g Asp G31y I Ie Ser Hi s Se r Ser Pr o Ala Asn Phe Val 130 135 140 Leu Val G31y Thr I'b t As n Pr o 31u O31u 31y O31u Leu Ar g Pr o O31n Leu 145 150 155 160 Leu As p Ar g Phe G31y Leu Ala Val As p Val Ala Ala Ser Thr Asn Pr o 165 170 175 (31u Val Ar g Val (31u I Ie I Ie Ar g Ar g Ar g Leu As p Phe 180 185 <210O> 371 <21 1> 601 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (601) <223> RXN00665 <400> 371 accaaacact t ct gt gcgt g acacgcgcca cct t at act c tcgggatctc aaagtttcga gaaacacaga aagggcagca ct t Le u ct c Le u at g vb t at g vb t gca A a gcg A a at g vb t gt t ValI gca A a ct g Le u c ac Hi s ct c Le u gcc A a ct c Le u ac c Thr g ac As p c ac Hi s 120 gt t ValI t ca Ser ccc Pr o tt c Ph e ct g Le u ct t Le u gca A a gga G31y g ac As p t cc Ser gga G31y t ca Ser at g vb t t t g Le u ggt G31y 75 ct c Le u ct c Le u ccc Pr o ct c Le u c aa O31n g at As p ac c Thr gt c ValI g ac As p ct g Le u tt c Ph e gca A a g ac As p 140 ac g Thr at c I Ie at c I Ie ac c Thr gcg A a tt c Ph e ccc Pr o 110 gt c ValI g ac As p gca A a 15 ct g Le u at g vb t at c I Ie ct t Le u gcg A a 95 ct g Le u gt c ValI tt a Le u gt a ValI ct g Le u tt c Ph e g ac As p 80 at t I Ie at c I Ie at c I Ie ccacaagcaa cacagaacac atg agc agc tca aca 11! I'b t Ser Ser Ser Thr 1 gcc gct gac tac acg 1& A a A a Asp Tyr Thr gt c ct t ggc t tc gcc 21 Val Leu G31y Phe Ala ggt cga ct C acc cca G31y Ar g Leu Thr Pr o ggt ctc atc gcc ggc O3 y Leu Il e A a 03 y gcc atc aag gac atg A a Il e Lys Asp I'b t at g t tc t tc gga at c I'b t Phe Phe G31y Ilie 1 00 cgc gtg atc acc cgc Ar g Val Ilie Thr Ar g 115 ggc acc gca gt a ct t 49! G31y Thr Ala Val Leu 130 ggc t cc acc acc t tc at c at t Oy Ser Thr Thr Phe Ilie Ilie 145 Page 445 seql i st cor r ect ed. t xt acc acc ttc cgc gat gct gcc cat cta cct gcg cct tgg cat gag ccc Thr Thr Phe Arg Asp Ala Ala His Leu Pro Ala Pro Trp His u Pro 150 155 160 165 tgt ggt Cys G y <210> 372 <211> 167 <212> PRT <213> Cor ynebact eri um gl ut am cum <400> 372 Met Ser Ser Ser Thr Leu Leu Leu Ala Ser Gy G n Val Thr Ala Leu 1 Al a Val Gy Gy Al a Met Ar g Gy Thr 145 Pr o Asp Gy Leu SIle Lys Phe SIle 115 Al a Phe Hi s 5 Thr Al a Pr o Gy Met I le Ar g Leu SIle Pr o 165 Leu bet Met Al a 70 Al a Met Val Al a Thr 150 Cys Ser SIle Val Gy Pr o SI e Leu Gy 135 Thr Gy Hi s Leu 40 Al a Leu Thr Asp Hi s 120 Val Phe Thr Thr Met Gy Al a Val 105 Asp Val Ar g Ser bet Leu Gy Leu Leu Pr o Leu Al a 155 Asp Thr Val Asp Leu Phe Al a Asp 140 Al a Gy Leu Pr o et Met Asp Lys 125 Gy Hi s Leu bet SIe Leu Al a Leu Val Gy Pr o <210> 373 <211> 390 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(367) <223> RXN03026 <400> 373 gttggcggcg cagtatcgtg aggtgcggga cctcgagcgg ggaatcccaa actagcatcc cgaactagcc ccccaacaac aattagaaat ggaacctaaa atg cct gga aaa att 115 IVet Pro Gy Lys Ile 1 ctc ctt ctc aac ggc cca aac ctg aac atg ctg ggc aaa cgc gag cct 163 Page 446 Le u g ac As p gct Al a c ac Hi s at c I Ie tt t Ph e Le u t ac Tyr gct Al a ggt G31y t gc cys at g vb t seqI i st car r ect ed. t xt G31y Pro Asn Leu Asn I'bt Leu G31y Lys 15 cac gac acc ttg gaa gac gtc gtc gcg Hi s Asp Thr Leu G31u Asp Val Val Al a aaa cac ggc ctt gag gtt gag gcg ctg Lys Hi s G31y Leu G31u Val G31u Al a Leu 45 ct a at c gat gcg ct g cac aac gct cgc Leu Ilie Asp Al a Leu Hi s Asn Al a Ar g at t aac ccc ggc ggc ct g act aca ct t Ilie Asn Pr o G31y G31y Leu Thr Thr Leu 75 t gaaggcgt c t gagct tcct acc G31u gca Al a ag c Ser ac c Thr t gg Tr p <210O> 374 <21 1> 89 <21 2> PRT <21 3> Cor ynebact er i urn gI ut anm curn <400> 374 I'bt Pro G31y Lys Ilie Leu Leu Leu Asn Gy Pro Asn Leu Asn I'bt Leu 1 5 10 G31y Lys Ar g G31u Pr o Asp I Ie Tyr G31y Hi s Asp Thr Leu G31u Asp Val 25 Val Al a Leu Al a Thr Al a G31u Al a Al a Lys Hi s G31y Leu G31u Val G31u 40 Al a Leu a3 n Ser As n Hi s a3 n a3 y a3 u Leu I I e Asp Al a Leu Hi s Asn 55 Al aAr g 1y Thr Hi s IlIea1y Cys Val IlIe As n Pr oa(1ya(1y Leu Thr 70 75 Thr Leu Ar g Tr p Ar g Phe Tr p I'b t Leu <210O> 375 <21 1> 384 <212> DNA <213> Cor ynebact er i urn gI ut anm curn <220> <221 OIDS <222> (361) <223> RXN02908 <400> 375 gccaacgagg gt tggt t tac cacct ct gat t caggt gaac t ccacgacgg gat tct cacc gt gact ggt c gcgt ggat ac ccgt cat tga t tccggt gga t tg aag t tg cac cca 115 Leu Lys Leu Hi s Proa 1 gag gt a ct g gaa cgt gcc at c gca gat at t aaa ggt gtc acc gcg gcg 163 Gu Val LeuOu Arg Ala lleAlaAsp Ilie Lys ay Val Thr Al aAl a Page 447 tgt Cys gcg Al a gat Asp ttg Leu ct g Leu seql i st cor r ect ed. t xt 15 gtg ggt att ccc gat ccc cga tta ggc caa gca Val Gy Ile Pro Asp Pro Arg Leu Gy On Al a tcc gga tcg atc agt ccg tct gaa gtt att gaa Ser y Ser Ile Ser Pro Ser u Val Ile u 45 cct cgt tgg cag ctt ccc aaa cgg ctg aag cat Pro Arg Trp G n Leu Pro Lys Arg Leu Lys His agc at t ggt cct gga aaa gct gat cga cgt gct Ser Ile y Pro y Lys Ala Asp Arg Arg Al a 75 tagtcttcat tcttgctggc tgc gtg Val ct c Leu gaa G u gcg Al a <210> 376 <211> 87 <212> PRT <213> Corynebacter i um gl <400> 376 Leu Lys Leu His Pro G u 1 5 Sy Val Thr Ala Ala Cys Sn Ala lie Val Ala Al a lie G u G y Leu Asp Asp Lys His Leu u Ser Leu 70 Arg Ala lie Ala Lys Leu ut ari cum Val Leu u Arg Ala lie Ala Asp Ile Lys 10 Val Val Gy lie Pro Asp Pro Arg Leu y 25 Tyr Ser y Ser lie Ser Pro Ser G u Val 40 Leu Pro Arg Trp G n Leu Pro Lys Arg Leu 55 Pro Ser lie y Pro y Lys Ala Asp Arg 75 Phe <210> 377 <211> 667 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(667) <223> RXN03000 <400> 377 cagcgtgttg ctgcgcctgc agaaaaccta cagcgtcacc cacaaagtcg aaatccaagg ctaaccccct ttttcaactc acagttagga aacttcaccc atg tct ctt cca cat 115 IVet Ser Leu Pro His 1 tct gat gaa ctc cgc ggc caa aag atc att att tcc ggt ggc gga att 163 Ser Asp u Leu Arg Gy On Lys lie lie lie Ser G y G y G y lie 15 Page 448 seql i st cor r ect ed. t xt ggt G y act Thr cag G n gac Asp gac Asp ttc Phe ct g Leu cac Hi s gag G u 150 ttg Leu cgc Ar g gca Al a t ac Tyr ggt Sy at t SIle gt c Val cag G n aac Asn 120 ggc Gy gac Asp gt c Val aag Lys ggt gca ctt gcg G y Al a Leu Al a cgc Ar g cac Hi s gt g Val cgc Ar g t ac Tyr ct g Leu ct g Leu gaa G u gcc Al a 170 gt c Val gca Al a ggc G y gcc Al a 75 gaa G u ggc G y gt c Val gt c Val t cc Ser 155 tt c Phe gac Asp gct Al a tgg Tr p ggc G y acc Thr ggt G y acc Thr acc Thr 140 t cc Ser ct c Leu gac Asp gag G u 45 cga Ar g t ac Tyr at c SIle cgc Ar g aac Asn 125 gat Asp at c SIle gcc Al a gcc Al a ctt Leu ttc Phe at g Met ct c Leu ct g Leu t ac Tyr 110 gcc Al a t cc Ser aac Asn ttc Phe gct ttg cgc ggt gcc Ala Leu Arg Gy Al a aag gag gtc ggc gct Lys G u Val Gy Ala ct gaa tcc tgg ggt Leu u Ser Trp y cca gaa gac atg cag Pro u Asp IVet G n acc atg cgt ttc gat Thr IVbt Arg Phe Asp 95 ctg gtg att cac cgc Leu Val Ile His Arg 115 gaa gca gcg ggc gcg G u Al a Al a G y Al a 130 cgc acc gtc gac ggc Arg Thr Val Asp y 145 aag ggc gaa gat aac Lys G y G u Asp Asn 160 gac ggc atc cac tcg Asp G y lie Hi s Ser 175 gat Asp ggc G y ctg Leu ttc Phe gaa G u 100 tct Ser aag Lys ggt Gy aag Lys gtc Val 180 <210> 378 <211> 189 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 378 IVet Ser Leu Pro His Ser Asp G u Leu 1 5 Ser y G y G y I e G y G y Al a Al a Arg y Ala Asp Val Thr Leu Tyr G u 40 Val y Ala y Leu n Ile y Pro 55 Ser Trp y Leu Leu Asp n Ile Val 70 Asp Iet G n Phe Arg Asp Ala Val Asn Arg G y G n 10 G y Al a Leu Ar g Al a Al a His G y Trp Val Ala G y Arg G u Thr Page 449 Lys Al a G u Ar g Tyr SIle I e Al a Lys Leu Pr o Thr Ar g SIle Al a Val 145 Su lie Asp Ar g 115 Al a Gy Asn Ser G u Asp Leu SIle Thr 165 Met G n Leu Asn 135 Val Leu Lys seql i st cor r ect ed. t xt 90 Sn His Tyr Gy y Arg 105 Asn I e Leu Val Thr Asn 120 125 Sy Val Leu Val Thr Asp 140 Asp Ile u Ser Ser Ile 155 Val Asp Ala Phe Leu Ala 170 Lys Leu Val Asp Asp Ala 185 Tyr 110 Al a Ser Asn Phe Leu G u Ar g Lys Asp 175 <210> 379 <211> 766 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(766) <223> RXN03036 <400> 379 tagaaaaatc tacccagtaa gcattcagga accattcaga tatcagcgta aacgtccgaa catgaaaggc tagaaaagcc atcttttctt agcatgtctc gct gag cag Al a G u G n cgt Ar g gat Asp gtt Val tgt Cys agc Ser at g Met ccg Pr o tcg Ser ttt Phe ggt Gy gga Gy cct Pr o gca Al a tcg Ser gt g Val gat Asp ggc Gy gga Gy at c SIle ggt Gy gca Al a act Thr gag G u gac Asp gt c Val gac Asp gtt Val t cc Ser acc Thr ggt Gy t cc Ser gt g Val cct aat caa Pro Asn n 15 gga acc gtt G y Thr Val gga gca ggt G y Al a G y gca tcg agc Al a Ser Ser gca gca gca Al a Al a Al a 80 tac ccg ggc Tyr Pro G y 95 gtg gag cgc Val G u Arg 110 gag ggc gta G u G y Val gag G u t cc Ser gca Al a t ca Ser act Thr ggt Gy gac Asp ttg Leu agg Ar g gct Al a gcg Al a act Thr aat Asn 100 acc Thr ttg Leu gaa G u aag Lys gct Al a t cc Ser gag G u ggt Gy aag Lys cct ctg acg ttg act Pro Leu Thr Leu Thr Page 450 seql i 120 125 atg acc att ttg gac atg aac aac aac IVet Thr Ile Leu Asp IVet Asn Asn Asn 135 140 gcg gtg tac gtg tgg cac tgt gat gcg Ala Val Tyr Val Trp His Cys Asp Ala 150 155 gac tct gag ctg gaa gat gag acc tat Asp Ser G u Leu G u Asp G u Thr Tyr 170 gat aag tat ggc cag gtc acg ttc gat Asp Lys Tyr Gy n Val Thr Phe Asp 185 190 gcg ggc cgt tgg gtg cat att cat ttc Ala y Arg Trp Val His Ile His Phe 200 205 agc atc acg gat tcc acg aac aac att Ser Ile Thr Asp Ser Thr Asn Asn Ile 215 220 <210> 380 <211> 222 <212> PRT <213> Corynebacter i um gl utam cum <400> 380 let Ala G u G n Leu Arg G n Phe G u 1 5 Ser G u Asp Leu G u Asp G n y Leu 25 Phe Ser Arg Arg Lys Val Leu y Phe 40 Val Ala Leu Ala Ala Cys Ser Pro Ser Thr Ser Ser Ala Ser Ser Ser Ala Ala 70 G u Thr Leu Thr G u IVet Lys Ser G u Asp G y Ser Asn G y Pr o Asp Val Leu 100 105 G n Asp Ile Thr Lys Ser Ile Asp Ser 115 120 Pro Leu Thr Leu Thr IVet Thr lie Leu 130 135 Pro lMt G u y Ala Al a Val Tyr Val 145 150 Arg Tyr Ser IVet Tyr Asp Ser G u Leu 165 G y Val n Ile Thr Asp Lys Tyr y 180 185 st cor r ect ed. t xt 130 aat cag cca at g Asn G n Pro IVet 145 ccg ggt cga tat Pro y Arg Tyr 160 tta cgc ggt gtg Leu Arg y Val 175 acc att ttc cct Thr lie Phe Pro gag gtg ttc ccg G u Val Phe Pro 210 G y Arg 10 G y Phe I e G y G y Ser Al a Thr Thr Al a 90 G u Val Asp Thr Asp IVet Trp Hi s 155 Su Asp 170 G n Val Leu Leu Sy Al a Ser Pr o Sy Al a 125 Asn Asp Thr gag G u tcg Ser cag G n ggt Sy 195 gat Asp Pr o Sy Sy Al a Al a Tyr Val 110 G u Asn Al a Tyr ggt G y at g let att SIe 180 tgt Cys cga Ar g Asn Thr Al a Ser Al a Pr o G u Sy Asn Pr o Leu 175 Thr Phe Asp Thr Ile 190 Page 451 seql i st cor r ect ed. t xt Phe Pro Gy Cys Tyr Al a Gy Arg Trp Val His Ile His Phe u Val 195 200 205 Phe Pro Asp Arg Asp Ser Ile Thr Asp Ser Thr Asn Asn Ile 210 215 220 <210> 381 <211> 318 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(295) <223> RXN02974 <400> 381 gcaggcatgg acacat t cca ggtcctgacc ggcgtcagcg gctactacga tttggtgc gccattccca gagcagcgcc ccacctatat cgccacctcg atg cag gat ctc tac gc agc gat ccg ggc gag ctc Ser Asp Pro Gy G u Leu ctt atc gac ggc gac acc Leu Ile Asp G y Asp Thr act ccg gtt gca gca ctc Thr Pro Val Ala Ala Leu aca gag cag tca ccg agg Thr G u G n Ser Pro Arg tgcat t gcag agc <210> 382 <211> <212> PRT <213> Corynebacter i um gl <400> 382 IVet G n Asp Leu Tyr Ser 1 5 G y G y Phe Ser Ala Leu G y Asp Al a G y Al a Thr Val Ala Trp Ala Ala Thr Ar g <210> 383 <211> 1017 aag Lys ct g Leu cgc Ar g t ac Tyr 60 cca Pr o gt c Val act Thr 45 gcg Al a ggt Gy at t I e 30 gcg Al a ct g Leu gcc Al a 15 t cc Ser ttg Leu at t SIle cag G n ggc Gy gat Asp cag G n IVet G n Asp Leu Tyr 1 ggc ggt ttt tca gcg G y G y Phe Ser Ala ggc gat gcc ggc gca G y Asp Al a G y Al a gtg gcc tgg gcg gcc Val Ala Trp Ala Ala agg t agct gct ac Ar g ut am cum Asp Pro Gy u Leu Lys Pro Gy Ala n 10 Ile Asp y Asp Thr Leu Val lie Ser y 25 Pro Val Ala Ala Leu Arg Thr Ala Leu Asp 40 G u G n Ser Pro Arg Tyr Ala Leu Ile G n 55 Page 452 seqi i st car r ect ed. t xt <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (994) <223> RXN00393 <400> 383 t ctat tcat t t cacaat agc gt t tcacact cccccat agc ct gccgaacg t at t tcaagc aat tgcgcga t cgagt at gt gat ggggaaa gat agaggt t at g t ct cac acg gaa l'bt Ser Hi s Thr O31u ccc Pr o ccg Pr o gt c ValI ct t Le u t ac Tyr cga Ar g gcg A a ct g Le u ggc O31y ct c Le u 1 50 gga O31y gcc A a aat As n ccg Pr o ac c Th r gct A a gt g ValI g at As p act Th r at t I Ie ag c Ser 120 t gg Tr p gag (31u c ag O31n gt t ValI cgc Ar g 200 t ct Ser gca A a cat Hi s t gg Tr p at t I Ie t ct Ser tt c Ph e t gg Tr p t ac Ty r gct A a ac c Th r 1 70 gt g ValI at t I Ie gt a ValI aat As n g at As p gct A a cgt Ar g 75 ggg G31y ggt G31y t gg Tr p acc Th r gt g ValI 155 caa O31n ggg G31y cca Pr o act Th r gcg A a ggt G31y tt g Le u ggc G31y tt g Le u at c I Ie ct g Le u ggc G31y 140 tt c Ph e ac c Th r t cg Ser ac c Th r tt g Le u tt c Ph e tt t Ph e 45 at c I Ie ac c Th r gct A a gca A a at c I Ie 125 ggt G31y at c I Ie ggt G31y at g vb t g at As p 205 t cc Ser gcg A a gt g ValI at c I Ie g at As p gag O31u ggt G31y 110 ct c Le u aaa Ly s tt c Ph e t cc Ser t ct Ser 1 90 ag c Ser t gg Tr p gt c ValI t gg Tr p gt g ValI g ac As p 80 aag Ly s gcc A a ggc G31y cct Pr a G31y 1 60 ag c Ser ggc G31y ac c Th r 1 at t I Ie at t I Ie aag Ly s aat As n 65 cgc Ar g aaa Ly s ggc G31y at c I Ie t at Ty r 145 ct c Le u t gg Tr p gt g ValI gga G31y c aa O31n gcc A a gcc A a 50 t ac Ty r ac c Th r gt g ValI ac c Th r ct g Le u 130 ggt G31y gt c ValI gcc A a aac As n aaa Ly s 210 ggc G31y ggt G31y 35 tt g Le u gcc A a ggt G31y aaa Ly s gcg A a 115 t gt cys t ac Ty r gcg A a ggt G31y tt g Le u 1 at t I Ie gca A a 20 t ca Ser ct g Le u aat As n cct Pr a gct A a 1 00 ct g Le u gt g ValI cgc Ar g gt c ValI tt g Le u 1 gcc A a ac c Th r cgc Ar g ggt G31y gcg A a g at As p ct g Le u gcg A a ag c Ser ct g Le u ggg G31y at g vb t 1 gcc A a aac As n ct c Le u gcg gt c cgc ct g ggc gat gcg ggt gct cgt aag ct g t tc ct c gcg ct g Page 453 seqI i st car r ect ed. t xt Al a Val Ar g Leu G31y Asp Ala Oy Ala Arg Lys Leu Phe 215 220 225 at t t cc acg ccg t tc at c at g t cc at c t gc ct g gcg tt t I Ie Ser Thr Pr o Phe I Ie I'b t Ser I Ie Cys Leu Al a Phe 230 235 240 cca gcg ct g at c gcg at c at c gt t t tc ccg ct g gca ct g Pr o Al a Leu Ilie Al a Ilie Ilie Val Phe Pr o Leu Al a Leu 250 255 ggg ccg at c cgc aac aac gcc acc ggc aag gat ct c at c O31y Pr o Ilie Ar g Asn Asn Al a Thr O31y Lys Asp Leu Ilie 265 270 ggc tca aca ggg cgc gcc atg gcg ttg tgg gcc gtg ctc G31y Ser Thr G31y Ar g Al a I'b t Al a Leu Tr p Al a Val Leu 280 285 290 gca t ta gcg t tt agc t aaaacgct t t tcgacgct c ccc Al a Leu Al a Phe Ser 295 <210> 384 <211> 298 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn Al a gcc Al a gcc Al a 260 gt c ValI G31y Le u t gg Tr p 245 gca Al a at c I Ie ct g Le u 1017 <400> 384 I'bt Ser H e 3 3 l a(Lys Al a L As n Ty r A Arg T r(Lys Val L 03 y Thr A 1 1 e Leu C 130 Ty r 03 y T 145 Leu Val A T p Al a3Val As n L s Th r 03 u ly Al a Ar g y Ser 03 y 35 eu Leu Al a I a As n As p ly Pro Leu 85 ys Al a Al a 1 00 l a Leu Ser ys Val Leu yr Ar g G31y I a Val Nib t 1 65 y Leu Al a 1 80 eu Al a Asn Pro On Pro Asn Pr a ValI Le u Tyr 70 Ar g Al a Le u G31y Le u 150 G31y Al a As n Ar g Al a ValI 55 Ser Le u Ph e Le u Al a 135 G31y Thr Al a I Ie Thr Al a 40 ValI As p Thr I Ie Ser 120 Tr p O31u O31n ValI Ar g 200 Tr p 25 Ph e Al a G31y G31y Ser 1 05 Al a Ph e I Ie Ph e G31y 1 85 As p Ser 10 Al a Hi s Tr p I Ie Ser 90 Ph e Tr p Tyr Al a Thr 1 70 ValI ValI As n As p Al a Ar g G31y G31y Tr p Thr ValI 1 O31n (31y Thr Al a O31y Le u O31y Le u I Ie Le u O31y 140 Ph e Thr Ser Le u Ph e Ph e li1e Thr Al a Al a I Ie 125 Oy le Oy vb t As p 205 Ser Al a ValI I Ie As p O31u O31y 110 Le u Ly s Ph e Ser Ser 1 As p Pr a Tr p O31y O31u Pr a ValI I Ie As n Ph e ValI 1 Al a I Ie Pro Thr Ser Lys Thr Page 454 seql i st cor r ec Gy Lys I e Thr Leu Ala Val Arg Leu Gy Asp 210 215 Leu Phe Leu Ala Leu I e Ser Thr Pro Phe Ile 225 230 235 Ala Phe Val Ala Trp Pro Ala Leu lie Ala lle 245 250 Ala Leu Lys Ala Ala G y Pro lie Arg Asn Asn 260 265 Leu Ile Pro Val Ile y Ser Thr y Arg Al a 275 280 Val Leu Thr G y Leu Al a Leu Al a Phe Ser 290 295 <210> 385 <211> 1242 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(1219) <223> RXN00948 <400> 385 acaccctcca aatgatctcg taaaacagta ttgaatttag tgccctcaag ccaagctagt tgtacgatca aactcgt t gt ted. txt Ala Gy Ala Arg Lys 220 IVet Ser I e Cys Leu 240 lie Val Phe Pro Leu 255 Al a Thr G y Lys Asp 270 IVet Ala Leu Trp Ala 285 gtacgactct aatcgtacct atg gca aac gtc gta 11! Met Ala Asn Val Val ct a Leu caa G n tgc Cys cag G n gtt Val ctt Leu gca Al a gct Al a gat Asp cgc Ar g t ac Tyr cat Hi s gca Al a ctt Leu at t l Ie cgc Ar g 120 at g Met at c SIle gca Al a gca Al a gga G y agc Ser gcc Al a gca Al a gag G u acc Thr gcc Al a gct Al a gt g Val 75 cat Hi s ggt G y t cc Ser cct Pr o at c SIle acc Thr cgc Ar g aac Asn gac Asp ggg G y acc Thr ttg Leu ccc Pr o ggt G y 45 gca Al a ccc Pr o caa G n gca Al a gat Asp 125 gt g Val aac Asn t ca Ser gca Al a gt a Val at t SIle gt a Val 110 gct Al a t cc Ser 15 cgc Ar g ggt G y ggt G y gct Al a gaa G u 95 ccg Pr o ccg Pr o aag Lys gt g Val ctt Leu ggt G y ccc Pr o 80 cca Pr o gcc Al a tgg Tr p ttt Phe at g Met acc Thr gga G y t cc Ser t cc Ser caa G n ggt Gy 130 acc Thr t ca Ser gat Asp tta Leu cca Pr o cga Ar g tta Leu 115 ggc Gy cca Pr o ccg Pr o ttt Phe gt c Val gt c Val gt g Val 100 gcc Al a gga Gy att SIe at g Met cac Hi s at g Met gac Asp aca Thr cat Hi s t at Tyr Page 455 seql i st cor r ect ed. t xt gtt gga cca Val Gy Pro 135 gca ttc cca Ala Phe Pro 150 caa gag gtt Sn G u Val gct ggt ttt Al a G y Phe cat aac ttc Hi s Asn Phe 200 gga tct tta G y Ser Leu 215 atc cgc gca I e Ar g Al a 230 acc acc gac Thr Thr Asp acg ctg agc Thr Leu Ser gtt gat ttg Val Asp Leu 280 ccg cat gac Pro His Asp 295 agt acc gga Ser Thr G y 310 act gcg cac Thr Al a Hi s ggc cgt cca G y Arg Pr o gca ct a ggt Al a Leu G y 360 acc Thr ttg Leu cag G n 170 gt c Val tct Ser aac Asn tgg Tr p gt g Val 250 agc Ser gat Asp gat Asp aca Thr ttg Leu 330 ct c Leu gaa G u aat Asn cct Pr o 155 cag G n gt g Val ccg Pr o cgc Ar g cca Pr o 235 gag G u agg Ar g gcc Al a tac Ty r gt c Val 315 gtt Val aag Lys ccc Pr o gga tgg gag act gtc ggc Sy Trp u Thr Val y 140 145 gct ccg cgc gag ctg acg Ala Pro Arg G u Leu Thr 160 ttc gct ggc gcc gcc gtt Phe Ala G y Al a Al a Val 175 gaa att cac gca gca cac G u Ile His Ala Ala Hi s 190 atc tcc aac aag cgc acc Ile Ser Asn Lys Arg Thr 205 gct cgc atc gtg ctc gaa Ala Arg lie Val Leu G u 220 225 gag gaa aag cct gta ttc G u G u Lys Pro Val Phe 240 gaa aac cca cag gat gat G u Asn Pro G n Asp Asp 255 cag ctg gct ttg tgg gca G n Leu Ala Leu Trp Ala 270 tct tct ggt ggc ctc gac Ser Ser G y G y Leu Asp 285 caa acc gcg aag gcc gca G n Thr Ala Lys Ala Ala 300 305 gct gct gtg ggg cgc at t Ala Ala Val G y Arg lie 320 gat tct ggc gat gtc aat Asp Ser G y Asp Val Asn 335 gat cct tcc tgg gca aac Asp Pro Ser Trp Ala Asn 350 agg tat gtt cac caa tac Arg Tyr Val His G n Tyr 365 ccc agc Pro Ser gtt tca Val Ser cgt gcc Arg Ala ggc tac G y Tyr 195 gat t ca Asp Ser 210 gtc att Val lile atg cgc IVet Arg cgc gag Arg G u t cc gag Ser G u 275 at c gt c Ile Val 290 gat ct t Asp Leu gat gac Asp Asp gca gtt Al a Val caa gca G n Al a 355 gac t ac Asp Tyr 370 cct Pr o gaa G u gat Asp 180 ctt Leu t ac Tyr gat Asp at t SIle t cc Ser 260 cac Hi s ccc Pr o cac Hi s gcc Al a ttc Phe 340 gcc Al a gt a Val ct g Leu at c SIe 165 cag G n ttg Leu ggc G y gca Al a t cc Ser 245 tgg Tr p gga G y at t SIe gca Al a caa G n 325 ct c Leu ct c Leu ctt Leu 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1242 taaaggagag ttgacatgaa ggt <210> 386 <211> 373 <212> PRT <213> Corynebacteri um gl utami cum Page 456 seqi i st car r ect ed. t xt <400> 386 vb t Le u Tr p Pr o ValI li1e Ph e ValI As n O31y 145 Thr ValI Hi s Thr O31u 225 Ph e As p A a As p A a 305 li1e As n As n Thr Ser As p Le u Pr o Ar g Le u 115 O31y Ser Ser A a Tyr 1 95 Ser I Ie Ar g O31u O31u 275 ValI Le u As p ValI ValI le vb t Hi s vb t As p Thr Hi s Tyr Le u I Ie 1 65 O31n Le u O31y A a Ser 245 Tr p Oy le A a O31n 325 Le u Leu Val Asp Arg (31n Cy s O31n ValI 70 Le u A a A a ValI A a 150 O31n A a Hi s Oy le 230 Thr Thr ValI Pr o Ser 310 Thr (31y I Ie Thr A a O31u O31y A a O31y O31y 135 Ph e O31u O31y As n Ser 215 Ar g Thr Le u As p Hi s 295 Thr A a Ar g Ar g Tyr 40 Hi s A a Le u I Ie Ar g 120 Pr o Pr a ValI Ph e Ph e 200 Le u A a As p Ser Le u 280 As p O31y Hi s Pr a As p Ser Tyr Thr Tr p Ar g 1 05 Ly s O31u O31y ValI As p 1 85 Le u O31u ValI Tr p O31n 265 li1e Ar g ValI As n Le u 345 vb t 10 lIe A a A a O31y Ser 90 A a A a Thr Le u O31n 1 70 ValI Ser As n Tr p ValI 250 Ser As p As p Thr Le u 330 O31u Pr o Leu Val Thr A a A a ValI 75 Hi s O31y Ser As n Pr a 1 55 O31n ValI Pr a Ar g Pr a 235 O31u Ar g A a Tyr ValI 315 ValI I Ie Thr Ar g As n As p O31y Thr O31y 140 A a Ph e Ou le A a 220 O31u O31u O31n Ser O31n 300 A a As p Pr o O31y A a Pr a O31n A a As p 125 Tr p Pr a A a I Ie Ser 205 Ar g O31u As n Le u Ser 285 Thr A a Ser As n Ser A a ValI I Ie ValI 110 A a O31u Ar g O31y Hi s 1 As n I Ie Ly s Pr a A a 270 O31y A a ValI O31y Ser Lys Ar g Val O3 y Leu Ala Pr o O31u Pr o Pr o Ala Pr o Tr p Thr Val O3 u Leu 1 A a A a 1 A a A a Lys Ar g Val Leu Pr o Val 240 O31n As p 255 Leu Trp O3 y Leu Lys A a O31y Ar g 320 As p Val 335 Leu Lys Asp Pro Ser Tr p Ala 350 Page 457 seql i st cor r ect ed. t xt Asn G n Ala Ala Leu Al a Leu G y Ala G u Pro Arg Tyr Val His G n 355 360 365 Tyr Asp Tyr Val Leu 370 <210> <211> <212> <213> <220> <221> <222> <223> 387 873 DNA Cor ynebact eri um gl ut am cum CDS (850) RXN01923 <400> 387 ccaaagt gaa taccccgact gcagcagcgc aaaagttcaa gtactttggg atgcaaatct agtagcacgt cccatgtttc tcacactctc aggagctgac t ct gca ctt Ser Al a Leu aaa ggt tca gga Lys G y Ser G y cct Pr o cat cat gtg gtt His His Val Val tta aat ggt tgg Leu Asn G y Trp ttt ggt Phe G y cat gct gcg Hi s Al a Al a tgg gga gct ttc Trp G y Al a Phe gct Al a gac tat ctt gac Asp Tyr Leu Asp ctc ggc aac Leu G y Asn aga aaa gac Arg Lys Asp tac acc tgg cac ttt tgg gat tac Tyr Thr Trp His Phe Trp Asp Tyr 45 cga ggt tac ggc Arg y Tyr y aac Asn gac gca Asp Al a gga gaa ttt act G y G u Phe Thr ct g Leu 60 gag gaa att tca G u G u lie Ser gcg Al a gat atc gtt gca Asp Ile Val Ala t ac Tyr at g Met atc gac tcg att Ile Asp Ser Ile ggt gga gtg ttc Gy y Val Phe gca gaa aag gtt Ala G u Lys Val t cc Ser atc ctg ggc cat I e Leu G y His atg cag aaa gt c 'bt G n Lys Val gca gac agc gcc Ala Asp Ser Ala acc ccc Thr Pro 100 atc gct t ca II e Al a Ser ctg gtt Leu Val 105 gga at t t ct G y i e Ser gcc Al a 110 gtt gct gca gct Val Ala Ala Ala gga aca cca Sy Thr Pro 115 ttc gat gag gat tct cgg aag ctt ttc acc tca gca ggg cac aac ccg Phe Asp G u Asp Ser Arg Lys Leu Phe Thr Ser Ala G y His Asn Pro 120 125 130 gac tcg Asp Ser 135 agg cga gcc atc Arg Arg Ala lle at c SIle 140 gat ttc acc tca Asp Phe Thr Ser gga G1y 145 tct cgc caa cct Ser Arg G n Pro gcc Al a 150 gcg tgg ttg gat Ala Trp Leu Asp ctc acc gac tcg Leu Thr Asp Ser gcg Al a 160 gtg cag aat tcc Val n Asn Ser cca gag gcc gtt Pro G u Ala Val gaa 10u 170 aag tac ttt ttt Lys Tyr Phe Phe tgg gct gat tgt Trp Ala Asp Cys aat ttc Asn Phe 180 Page 458 gca A a g at As p ccg Pr o t ac Tyr 230 tt t Ph e g at As p g ac As p 200 t at Tyr at t I Ie g ac As p ggc gcg A a aat As n gag (31u gt c ValI 250 seqI i st car r ect ed. t xt acc caa gat t tg ccc gt g gac at t ct c acc Thr O31n Asp Leu Pr o Val Asp I Ie Leu Thr 190 195 gt c act aaa act gcc gt g gaa t cc gca tt c Val Thr Lys Thr A a Val 03 u Ser A a Phe 205 210 ct g acc gt t gaa gaa ct c cac gat gt c gga Leu Thr Val O31u O31u Leu Hi s Asp Val G31y 220 225 cac ccc t ta ggc ct t gcc gcc agg gt g ct t Hi s Pr o Leu G31y Leu Ala Ala Ar g Val Leu 235 240 t agt act tcc gcaaattcac cgg <210O> 388 <21 1> 250 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 388 I'bt Ser A a Leu Il e Lys y Ser 0 y As n Le u A a li1e As p A a A a G31y 145 ValI A a As p (31u Tr p Le u Ar g I Ie G31y A a G31y 115 Hi s Ar g As n cys Le u 1 95 A a G31y As n As p A a Ser Pr o Pr o Pr o Pr a Thr 1 65 Ph e G31y (31y Hi s Tyr As p Tyr 70 vb t I Ie Ph e As p A a 150 Pr a A a As p Pr a A a Thr A a li1e G31y A a As p Ser 135 A a (31u A a Le u I Ie 215 A a Tr p 40 G31y As p G31y Ser (31u 120 Ar g Tr p A a As p As p 200 Tyr G31y Hi s (31u Ser ValI Le u 1 05 As p Ar g Le u ValI Le u 1 85 Pr a (31n Pr a 10 Tr p Ph e Ph e I Ie Ph e 90 ValI Ser A a As p (31u 1 70 G31y A a Hi s G31y Tr p Thr (31u vb t G31y Ar g I Ie As p 1 Ly s Thr ValI Hi s A a As p Le u A a O31n I Ie Ly s I Ie 140 Le u Tyr O31n Thr ValI Ph e Tyr (31u (31u Ly s Ser Le u 125 As p Thr Ph e As p Ly s 205 ValI A a Ar g (31u Ly s ValI A a 110 Ph e Ph e As p Ph e Le u 1 Thr ValI As p Gy le ValI Le u ValI Thr Thr Ser A a 1 Pr a A a Asn Leu Thr Val (3u (3u Leu 220 Page 459 seqi i st car r ect ed. t xt Hi s As p Va 1G31y Hi s Ty r Al a I Ie Ph e O31u Hi s Pr o L eu G31y L eu Al a 225 230 235 240 Al a Ar g Val Leu Ar g Phe Leu Asp Ala Val 245 250 <210O> 389 <21 1> 873 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 CDS <222> (850) <223> RXN00398 <400> 389 tgagttcgcc accatcagca ccggcaccca ccagcgcggt t gt ct cccgt ct gcct gaag cacct aagga aaact aaat c gt ggt taacc gt gagaagt t at g gcc aag t tg t tt 11! l'bt Al a Lys Leu Phe g at As p aac As n gt t ValI c ag O31n cca Pr o gag (31u tt g Le u cgc Ar g gct Al a gt c ValI 1 50 ct t Le u cat Hi s t ac Tyr G31y tt c Ph e t at Tyr ct t Le u cgc Ar g gt c ValI 120 g aa (31u at t I Ie tt g Le u cat Hi s ccc Pr o g aa O31u c ag O31n ggt G31y ct g Le u ggt G31y g ac As p g ac As p c ac Hi s g aa (31u 1 70 at c I Ie gag O31u gt t ValI ggc G31y gt c ValI 75 gac As p t cg Ser ct a Le u gag O31u t t a Le u 155 aat As n at c I Ie cct Pr o gct Al a t ac Tyr act Thr aaa Lys5 gca Al a gcc Al a tt g Le u 140 ggg G31y ggc (31y g at As p tt c Ph e gcc Al a 45 ct c Le u c ag O31n gct Al a ggt G31y ggt G31y 125 g aa (31u ct c Le u at t I Ie ccc Pr o ac c Thr gga G31y aaa Lys5 at c I Ie ggc G31y ct t Le u 110 t gg Tr p t ca Ser c ac Hi s aaa Lys5 c ac Hi s gag (31u at t I Ie gct Al a gca Al a 80 aag Lys5 g at As p ac c Thr tt g Le u g at As p 1 60 aaa Lys5 1 cca Pr a g at As p gt t ValI ct c Le u 65 g at As p gct Al a ct c Le u g aa (31u gcc Al a 145 gga G31y gca Al a ct g Le u t ac Tyr t cc Ser 50 gca Al a ac c Thr gt g ValI gag (31u ct c Le u 130 t cc Ser ct t Le u g aa (31u 20 gcg Al a t ct Ser ct t Le u g at As p tt a Le u 1 00 tt g Le u gt g ValI cct Pr a gca Al a aac As n cgt Ar g tt c Ph e ggc G31y c ag O31n aac As n gca Al a g at As p gct Al a ct t Le u 1 acc gga t tc gga Thr 03 y Ph e 03 y 1 Page 460 cgg Ar g g at As p aag Ly s ggc G31y 230 t ac Tyr g aa (31u act Thr 200 cct Pr a g at As p gga (31y g at As p tt g Le u g aa (31u gt c ValI c ag O31n 250 seqi i st car r ect ed. t xt cca act gaa gt c at c cag gca at c at g gct Pr oThr 31u Val IlIe (1n Ala I Ie 'b t Ala 190 195 at g at c gga act gat ct t cca t cc acc cgc l'b t I Ie G31y Thr As p Leu Pr o Ser Thr Ar g 205 210 gac gct gac ct a gat t tg at c gct gaa acg As p A a As p Leu As p Leu I I e A a 03 u Thr 220 225 gac aac gtc ttc tgg aac aac gct gca gcg Asp Asn Val Phe Tr pAsn Asn AlaAla Ala 235 240 t agtt t taag acccgaaat g t ct <210O> 390 <21 1> 250 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ari cumn <400> 390 l'bt A a Lys Leu Phe Asp Ser Hi s Phe Pr a As p ValI Le u As p A a Le u O31u A a 145 G31y A a A a I Ie Th r G31y ValI Ser Ar g Th r 115 Ty r Le u Pr a G31y vb t 1 95 As n Ar g Ph e G31y O31n As n A a As p A a Le u 1 65 G31y ValI As n ValI O31n Pr a 70 O31u Le u Ar g A a ValI 150 Le u Ar g As p G31y O31u A a G31y I Ie Ly s Ar g Ar g 135 Ser Ar g ValI Pr a Le u Le u As p ValI As p G31y 1 Hi s Le u As p ValI Le u 1 A a I Ie O31u ValI G31y ValI As p Ser Le u O31u Le u 1 As n Pr a vb t I Ie Pr a A a Ty r Th r Ly s A a A a Le u 140 G31y G31y Th r I Ie As p Ph e A a Le u O31n A a G31y G31y 125 O31u Le u I Ie O31u G31y 205 O31n ValI A a As p Pr a ValI As p Hi s Th r Ar g ValI 1 li1e As p Pr o Ser Thr Ar g Thr Lys Ar g Pr o Phe O31u Asp Ala Asp Leu Asp Leu Page 461 seqi i st car r ect ed. t xt 210 215 220 1l1e Ala 1u Thr Val O31y 1u Asp His Val AspAsn Val PheTrpAsn 225 230 235 240 Asn A a A a A a Phe Tyr Leu 03 y As p 03 n 245 250 <210O> 391 <21 1> 1108 <21 2> DNA <213> Cor ynebact er i urn gi ut anm cumn <220> <221 ODS <222> (1099) <223> RXN02813 <400> 391 ggact ct aaa t tgacccgat ct t tat act c cgacct tgct cagcct t tcc gacgagact t t cct aaagaa t ct tct tgt c ggt agt ggag aacacct cag gt g gag gcc gct t tg 11! Val Ou Ala Ala Leu gcg A a g at As p ggc G31y ccg Pr o gcg A a ct t Le u gca A a ac g Thr gca A a ag c Ser 1 50 aag Ly s gca A a t at Tyr aat As n ggc at g vb t aag Ly s ccg Pr o 120 gcc A a aat As n gcc A a gcc A a tt g Le u ct c Le u c ac Hi s t gc cys gcg A a at g vb t ct g Le u gag O31u ac g Thr gag (31u ccc Pr o g at As p at t I Ie at t I Ie at g vb t 75 cga Ar g ggg G31y acc Thr gcg A a gcg A a 155 t t g Le u gag O31u gt g ValI ccg Pr o aag Ly s g at As p cgc Ar g ggc ct g Le u 140 gt g ValI ggc G31y c ac Hi s gag O31u ct g Le u 45 gca A a g aa O31u tt g Le u ac g Thr ggc 125 gag O31u c ac Hi s ct t Le u gca A a gag O31u gt c ValI ac g Thr ggg G31y gcc A a tt g Le u 110 t gg Tr p tt t Ph e ccg Pr a tt t Ph e gca A a 15 ct t Le u act Thr ag c Ser gt g ValI gag O31u 95 ggg G31y ct g Le u ccg Pr a cgt Ar g at g vb t t cc Ser g ac As p c ag O31n ggg G31y 80 ct c Le u c ag O31n gt t ValI gt g ValI ggc G31y 1 60 1 gcg A a cgt Ar g ct c Le u g ac As p 65 gag O31u ac c Thr at c I Ie gcg A a t cg Ser 145 tt c Ph e aag Ly s cgc Ar g aag Ly s 50 at c I Ie gt c ValI gcg A a gcg A a gt g ValI 130 t at Tyr gag O31u gcc A a gca A a 35 gcc A a at t I Ie gt c ValI gag O31u ac g Thr 115 g ac As p ggc G31y at t I Ie ac c Thr 20 gcc A a at c I Ie g at As p g ac As p cat Hi s 1 00 ccg Pr a aat As n ggt G31y c ag O31n at t I Ie gag (31u aat As n t ct Ser aag Ly s aaa Ly s ac g Thr gcg A a gcc A a gcg A a 1 gat ccg cag t gg gt g t gg cat Asp Pro On Trp Val Trp His Page 462 IO seql i st cor r ect ed. t xt O 170 175 180 tcc gat cgc acg ccg atc act gcg atc gcg tcg gcg ctg gca acg gcc 691 Ser Asp Arg Thr Pro Ile Thr Ala lie Ala Ser Ala Leu Ala Thr Ala 185 190 195 d) 4 gct ggt gtg gta cgc aaa att gct ggt gac gtg gtg ttt tac tca caa 739 Ala Gy Val Val Arg Lys Ile Ala G y Asp Val Val Phe Tyr Ser Q n 200 205 210 acc gag gtc ggc gag ttg cgg gag aaa tcc ccc ggc ggc agc tcc gcg 787 Thr G u Val Gy G u Leu Arg G u Lys Ser Pro G y y Ser Ser Ala C1 215 220 225 00 atg ccc cac aaa gcc aat ccg gcc gct gcg att gcg tgc gac ggt tac 835 SIVet Pro His Lys Ala Asn Pro Ala Ala Ala lie Ala Cys Asp G y Tyr S230 235 240 245 (N gcg cgc cgg gca cct ggc ctt ctt gca acg ctt ttc gac gcc ctc gac 883 SAla Arg Arg Ala Pro y Leu Leu Ala Thr Leu Phe Asp Ala Leu Asp 250 255 260 (N tgc cgt ttg cag cgc ggc acc ggc agc tgg cac gcg gag tgg gca acg 931 Cys Arg Leu G n Arg G y Thr G y Ser Trp His Ala G u Trp Ala Thr 265 270 275 ctg cgc gag ttg gct gct gtc act cac tca gca gtg agc agg gct gca 979 Leu Arg G u Leu Ala Al a Val Thr His Ser Ala Val Ser Arg Ala Ala 280 285 290 acc agc atc gat ggc atc acc gtc aac gtt gat gtg atg gca agt cgc 1027 Thr Ser Ile Asp G y Ile Thr Val Asn Val Asp Val IVet Al a Ser Arg 295 300 305 gtc aat gga cca acc ggg cac gcc gaa gat ttg gcg gag cgg gca cta 1075 Val Asn G y Pro Thr G y His Ala G u Asp Leu Ala G u Arg Ala Leu 310 315 320 325 gaa att tat gga aaa gga cgc agt taatggatc 1108 Su Ile Tyr y Lys y Arg Ser 330 <210> 392 <211> 333 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 392 Val G u Ala Ala Leu Ala Val Ala Ala Ala Pro u His Ala Ala Met 1 5 10 Ala Lys Ala Thr lie Asp Ser Tyr G n Leu Asp Val G u G u Leu Ser 25 Arg Arg Ala Ala G u G y y Asn Pro Leu lie Pro Leu Val Thr Asp 40 Leu Lys Al a lie Asn Pro Al a G y lie His lie G y Al a Thr Ser G n 55 Asp lie lie Asp Ser Ala Leu IVet Leu Cys IVet Lys G u y Val y 70 75 G u Val Val Asp Lys Leu Lys Lys Leu Ala Arg Asp Leu Ala G u Leu 90 Thr Al a G u Hi s Lys Al a Thr Pro I e Iet G y Arg Thr Leu G y G n Page 463 seql i st cor r ect ed. t xt 100 Ile Ala Thr Pro Thr Thr Phe 105 Ala Leu Thr Al a Ser 145 Phe G n Al a Val Gy 225 Al a Phe Al a Val Val 305 Al a 115 Asp Gy I e Val Al a 195 Tyr Ser Asp Al a Tr p 275 Ar g Al a Ar g Al a Al a Al a 165 Hi s Al a G n Al a Tyr 245 Asp Thr Al a Ar g Leu 325 Al a Ser 150 Lys Ser Al a Thr Ibet 230 Al a Cys Leu Thr Val 310 G u Ar g 135 Gy Leu Asp Gy G u 215 Pr o Ar g Ar g Ar g Ser 295 Asn SIle Gy 120 Al a Asn Al a Ar g Val 200 Val Hi s Ar g Leu G u 280 I le Gy Ty r Leu Met G u Thr 185 Val Gy Lys Al a G n 265 Leu Asp Pr o Gy Al a Al a 155 Leu SIle Lys Leu Asn 235 Gy Gy Al a SIle Gy 315 Gy G y G y 125 Leu G u 140 Val Hi s G y Leu Thr Al a I e Al a 205 Arg G u 220 Pro Al a Leu Leu Thr G y Val Thr 285 Thr Val 300 Hi s Al a Arg Ser Tr p Phe Pr o Phe l Ie 190 Gy Lys Al a Al a Ser 270 Hi s Asn G u Leu Pr o Ar g Asp 175 Al a Asp Ser Al a Thr 255 Tr p Ser Val Asp <210> 393 <211> 1218 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1195) <223> RXN00136 <400> 393 cagtgttgca tcatctagaa atcgattaat taaaccgggc acctgattaa cattgggctg cccggtttct tcctattaca agcgaaaggc aacgtgcccc atg agc gca gtg cag 115 Met Ser Ala Val G n 1 att ttc aac acc gtc cac gtc aat gga tct tcc ccc tat gat gtc cac 163 lie Phe Asn Thr Val His Val Asn Gy Ser Ser Pro Tyr Asp Val His 15 att ggt tcc ggc ctc aac gag ctc att gtt cag cgc gca gcg gaa tca 211 I le y Ser y Leu Asn u Leu I e Val n Arg Ala Al a u Ser Page 464 seql i st cor r ect ed. t xt ggc Gy gca Al a cac Hi s ggg Gy at c SIle gt c Val acc Thr aat Asn 150 gca Al a at c SIle cca Pr o aaa Lys acc Thr 230 cgc Ar g cgc Ar g at g Met ct c Leu gag Gu gag G u aat Asn tgc Cys at c SIle gct Al a 120 ttg Leu gcc Al a ttc Phe gcg Al a at c SIle 200 gt c Val aag Lys at c SIle aac Asn at c SIle 280 gag Gu gt a Val gat Asp ccc Pr o gat Asp ctt Leu tgg Tr p at g Met ggc Gy gac Asp 170 t cc Ser cgc Ar g ggc Gy t cc Ser aac Asn 250 cgc Ar g aac Asn cac Hi s gcg Al a gca Al a gat Asp 75 gaa G u ggt Gy at g bet gt g Val aag Lys 155 acc Thr gcc Al a ctt Leu t cc Ser gt g Val 235 tac Tyr tgg Tr p ct c Leu cgc Ar g at t l Ie gca Al a gcg Al a ttg Leu ggc Gy cgt Ar g gac Asp 140 aac Asn gat Asp gaa G u t ac Tyr cac Hi s 220 gt c Val gga Gy cgc Ar g t cc Ser t ca Ser ttg Leu 45 ct a Leu gaa G u ggt Gy ggt Gy ggc Gy 125 gct Al a ctt Leu cgc Ar g at c SIle gaa G u 205 ct a Leu ggc Gy cac Hi s cac Hi s cac Hi s 285 at c SIle cac cag ccc His G n Pr o gtc gct gct Val Al a Al a aac ggc aag Asn G y Lys ggc gca gca G y Al a Al a 95 gct gcc aca Al a Al a Thr 110 gtg cgc gt c Val Arg Val gcg gtg ggc Ala Val y gtg ggc gcg Val Gy Al a 160 ct a gcc acc Leu Al a Thr 175 at c aaa act Ile Lys Thr 190 act gat ccc Thr Asp Pro cct gaa ct g Pro G u Leu caa gac ct c G n Asp Leu 240 acc ttt gcc Thr Phe Al a 255 ggc aat gcc G y Asn Al a 270 aag ctc ggg Lys Leu G y ctg gcg gcc Leu Al a Al a agc Ser ggt Gy t cc Ser ttc Phe gat Asp at t SIle ggc Gy 145 ttc Phe ct g Leu ggt Gy gca Al a at t SIle 225 aaa Lys cac Hi s gtt Val ctt Leu at g Met ttg Leu ttg Leu ggc Gy ct c Leu cag G n 130 aag Lys cac Hi s cct Pr o ttc Phe gcc Al a 210 tgg Tr p gaa G u gcc Al a gca Al a at c SIle 290 gat Asp aag Lys gaa G u cgc Ar g gcg Al a 115 gtt Val act Thr gag G u gac Asp at c SIle 195 tgc Cys cgc Ar g tct Ser gt c Val gt g Val 275 gac Asp gac Asp gt c Val gt a Val cgc Ar g 100 gga Gy cca Pr o ggc Gy cct Pr o gcg Al a 180 gcc Al a ct g Leu t cc Ser agc Ser gaa G u 260 ggc Gy gcg Al a 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 atc ggt ctg ccc act Ile Gy Leu Pro Thr Page 465 t cc Ser 310 g ac As p gt g ValI agt Ser g aa O31u aaa Ly s gag O31u t at Tyr 360 gga O31y cgc Ar g 330 ac c Thr gca A a gcc A a 315 gac As p cgc Ar g at c I Ie 300 t tc Ph e ggc O31y at t I Ie ag c Ser seqI i st car r ect ed. t xt 305 gac gag ct t t ac gac ggt at g acc cgc Asp O31u Leu Ty r As p 31y I'b t Thr Ar g 320 325 aac at c cgc t tc gt c gca ct g acc gcc As n I Ie Ar g Phe Val Ala Leu Thr Ala 335 340 gag ggg ccc tca aaa caa gat tta cag O31u G31y Pr o Ser Lys O31n Asp Leu O31n 350 355 cac t aagt gt t ga gt aat ct act agt Hi s 365 1 075 1123 1171 1218 <210O> 394 <21 1> 365 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ani cumn <400> 394 [vbt Ser A Pr o Ty r A,, Ar g Ala A Ser I'b t A,, 03 y Leu L~ Ser Leu 03 Phe 03 y Ar Asp Leu A 11 Ile 03 n V, 130 O3 y Lys T~ 145 Phe Hi s 03 Leu Pr o A,, O3 y Phe I I A a A a CQ 210 a Val ~p Val 2C aO(1L 3p As r (s Val u Val g Ar c aO '5 1l Pr c u Pr c ~p A E 1 8C e A E (s LeL G n Hi s Ser I Ie Le u A a As p Ph e Thr I Ie As p 1 65 0Ou As p Ly s I I e Phe Asn Thr G31y A a Ser 55 Le u O31n ValI A a Le u 135 Thr ValI I Ie O31u O31u 215 Ser O31u 40 O31u As n cys I Ie A a 120 Le u A a Ph e A a I Ie 200 ValI G31y 25 O31n Le u ValI Tr p O31y 1 05 A a A a A a I Ie O31y 1 85 Le u O31u Hi s As n A a A a As p 75 O31u O31y vb t ValI Ly s 1 55 Thr A a Le u Ser ValI Ou le A a A a Le u O31y Ar g As p 140 As n As p O31u Tyr Hi s 220 As n Le u Le u Le u O31u O31y O31y O31y 125 A a Le u Ar g I Ie O31u 205 Le u G y lIe Hi s ValI As n 0Oy A a 110 ValI A a ValI Le u I Ie 1 Thr Pr o Ser ValI O31n A a O31y A a A a Ar g ValI O31y A a 1 Ly s As p O31u Ser O31n Pr o A a Ly s A a Thr ValI O31y A a 1 Th r Th r Pr o Le u i~e Trp Arg Ser Val Thr Val Lys Oy Ser Val Val O31y O31n Asp Leu Page 466 225 Ly s Hi s ValI Le u I Ie 305 As p ValI Ly s Ser ValI ValI 275 As p Le u vb t Le u As p 355 Le u 245 Le u vb t Pr o Thr Ar g 325 A a O n (31u G31u Ph e Le u 295 Tyr Ly s G31y A a seql i st car r ect ed. t xt 235 Il e Leu Asn Tyr 0 y Hi s 250 As n Phe Ar g Tr p Ar g Hi s 265 li1e Ala Asn Leu Ser Hi s 280 285 (31u Ar g Hi s Ar g Ser I Ie 300 03 u 03 y 03 y A a Phe As p 315 Lys As n Ar g As p G31y As n 330 (31u Val Thr Ar g I Ie (31u 345 Ty r (31u Ala I Ie Ser Hi s 360 365 Ph e 255 As n Le u A a Le u Ar g 335 Pr o 240 A a A a G31y A a Tyr 320 Ph e Ser <210O> 395 <21 1> 1977 <21 2> DNA <21 3> Cor ynebact er i urn gI ut ani cumn <220> <221 CIDS <222> (1954) <223> RXN02508 <400> 395 t gcacact gc t ggt ggt gag gccgcagacc t gg aact cgcggc t cagt aaaac caaaaggaat ct t gcc A a gca A a gt t ValI gga G31y g aa (31u aag gt t ValI gct A a ccg Pr o ac c Th r gag (31u at g vb t tt g Le u tt t Ph e t cg Ser g at As p tt t Ph e ag g Ar g t cc Ser g at As p gca A a ct g Le u ct g Le u 75 ct t Le u gga G31y ggt G31y gag (31u tt c Ph e aag Lys5 (31y ct t Le u g aa (31u at t I Ie ccg Pr o ct g Le u gag (31u ;cagccgc aagcaaagcc tccgaggccc tgaccac at g cgt aca t cc at t I'b t Ar g Thr Ser Ilie 1 gct gaa aag ct g cgc gca gct Ala (31u Lys Leu Arg Ala Ala 15 at c t tc gag cag gac t tg gt g Il e Phe 03 u 03 n Asp Leu Val cgt cag cgg gct cag gat tt g Ar g O31n Ar g Ala O31n Asp Leu t tt cga gat t tc gaa ggt gt g Phe Ar g Asp Phe O31u G31y Val cac cgc t tg gaa gag aag tt c Hi s Ar g Leu 031u 031u Lys Phe 80 at g at c t tg t tg t gt t cc aat I'b t Ilie Leu Leu Cys Ser Asn 95 100 Page 467 gtg ggc acc gcg acc atc Val Gy Thr Ala Thr I le 105 seql i st cor r ect ed. t xt aat gat gat gac ctt ttc gt Asn Asp Asp Asp Leu Phe Va 110 g gag cag ttg I Gu n Leu cat Hi s gaa G u gca Al a 150 acg Thr at c SIle ct g Leu ggt Gy acg Thr 230 cgc Ar g cgt Ar g cca Pr o ggt Gy cgg Ar g 310 aag Lys tgt Cys gca Al a 120 ttg Leu gt g Val cat Hi s gcg Al a at g Met 200 ggc Gy t at Tyr gcc Al a ttg Leu gct Al a 280 gat Asp ctt Leu gat Asp cgt Ar g gat Asp tgg Tr p aag Lys ctt Leu 170 aag Lys at t SIle ttc Phe ggc Gy gtt Val 250 gat Asp gaa G u at t SIle caa G n cag G n 330 gt c Val gct Al a aag Lys 140 aat Asn cgt Ar g ttc Phe t cc Ser ct g Leu 220 at t SIle cgc Ar g acc Thr cgt Ar g at c SIle 300 ggt Gy tgg Tr p ggg Gy gag G u 125 ttt Phe cac Hi s ggt Gy ttt Phe tgg Tr p 205 gt g Val tct Ser acc Thr tgg Tr p gca Al a 285 gcc Al a ttc Phe acc Thr gct Al a gct Al a 365 aag t ac Lys Tyr gt c aat Val Asn aag gcg Lys Al a tgg gaa Trp u 175 gtt cag Val G n 190 t cg cgt Ser Arg aaa ttc Lys Phe ttg gag Leu G u gcg att Ala I e 255 cat gcg Hi s Al a 270 ctt cct Leu Pro act gga Thr G y cgc ttg Arg Leu cag ggc G n y 335 cca acc Pro Thr 350 gct cat Al a Hi s aac Asn gat Asp ct g Leu 160 acc Thr tta Leu cac Hi s at g Met at c SIle 240 gat Asp ct a Leu gag G u cgt Ar g ggt Gy 320 gat Asp acg Thr gcc Al a aag Lys 130 gag G u acc Thr gag G u gat Asp cgt Ar g 210 cat Hi s aac Asn ttg Leu gct Al a gcg Al a 290 ggt Gy cat Hi s cgc Ar g tct Ser gcg Al a 370 115 at t SIle cat Hi s tgc Cys gt g Val gcg Al a 195 gtt Val ct g Leu gat Asp cgt Ar g gag G u 275 gaa G u gag G u cac Hi s at t SIle gcg Al a 355 gaa G u gct Al a gcg Al a ttg Leu gag G u 180 ccg Pr o ttc Phe gcc Al a t cc Ser tct Ser 260 gat Asp cct Pr o acc Thr tgc Cys gt g Val 340 at g Let ttg Leu 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 ttt gac acc Phe Asp Thr 360 ccc gat cca gaa Pro Asp Pro O u Page 468 seql i st cor r ect ed. t xt cgg gct cag aca att gat cgt Arg Ala n Thr Ile Asp Arg 375 380 ccc cac at c Pro His Ile gag ggc gaa gtt gac cta G u Val Asp Leu Su aaa ggt gt Lys G y Vc 390 agc ccc gc Ser Pro A, caa gaa gc Sn G u Al ccg tgg c Pro Trp G 4L gcg ttg gc Al a Leu G 455 gtg cgc ac Val Arg A 470 ct c agc gt Leu Ser V, gcc tac cc Al a Tyr Pr gct gaa cc Al a G u Ar aat tac t Asn Tyr T} 535 ttg gac ac Leu Asp T\ 550 ggc gaa tt G y G u P\ gaa gtg gt Su Val V, gct ccg gt Al a Pro V, 6( cgg gga at Arg Gy II 615 <210> 396 <211> 618 <212> PRT g tac gca al Tyr Ala at gga at g sp G y IVet 410 ct ggt ctc a G y Leu 425 aa cat ttt n Hi s Phe 40 ag act gtg u Thr Val at cag gtg sn G n Val g gcg ccc al Ala Pro 490 cg gag cac ro u His 505 gt gcg cgc rg Al a Ar g 20 ac gac gat yr Asp Asp ca ct c aag hr Leu Lys c ctc cac he Leu Hi s 570 g gaa cgc al G u Arg 585 g cgg ttg al Arg Leu )00 c cca aac e Pro Asn ccg Pr o 395 ccc Pr o at t SIle gat Asp cgt Ar g at g bet 475 gag G u at t SIle aaa Lys gt g Val gaa G u 555 ttt Phe cgc Ar g gcg Al a gat Asp gag G u gaa ggu gag G u gag G u 460 cgt Ar g gac Asp gcg Al a cga Ar g cag G n 540 aac Asn t ac Tyr ggc Gy gcg Al a ggg Gy tgg Tr p gcc Al a gca Al a 445 gat Asp tcg Ser ggt Gy ttg Leu ggc Gy 525 gcg Al a cac Hi s acc Thr ggt Gy cag G n 605 gtg gag ct g Val G u Leu 400 ctg ccg gaa Leu Pro G u 415 atc gac cac I e Asp Hi s 430 gtg ctg ttt Val Leu Phe gag ttc ccg G u Phe Pr o ccg aat gat Pro Asn Asp 480 gag cag gga Su n y 495 gcc acg gcg Al a Thr Al a 510 ctg gat ttc Leu Asp Phe cgt ttt gat Arg Phe Asp ctg ctt tac Leu Leu Tyr 560 cgc acg ttg Arg Thr Leu 575 ttt gca ggt Phe Al a G y 590 tat cgt gag Tyr Arg G u uy 385 ttt Phe ttc Phe gt c Val t ac Tyr agc Ser 465 gcg Al a gat Asp gac Asp ttg Leu ttg Leu 545 gac Asp ggc Gy tgg Tr p gt g Val ttc Phe ggc Gy aat Asn acc Thr 450 cca Pr o gt g Val ttc Phe at c SIle ccc Pr o 530 ccg Pr o cgc Ar g acg Thr ggc Gy cgg Ar g 610 gcg Al a gt c Val ttc Phe 435 gcg Al a at t SIle cgg Ar g ct c Leu gt g Val 515 gt c Val cag G n gac Asp ct g Leu gaa G u 595 gac Asp ggg Gy gaa G u 420 gcc Al a ct g Leu ggt Gy ttg Leu aac Asn 500 gcg Al a cca Pr o gaa G u gag G u ttc Phe 580 aca Thr ct c Leu 1267 1315 1363 1411 1459 1507 1555 1603 1651 1699 1747 1795 1843 1891 1939 1977 tagcatcccg aactagcccc cca Page 469 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut ai cum <400> 396 Met Arg Thr Ser 1 Lys Leu Arg Ala G u G n Asp Leu Arg Ala G n Asp Asp Phe G u G y Leu G u G u Lys Leu Leu Cys Ser 100 Phe Val G u G n 115 Val Lys Ile Al a 130 Phe G u His Al a 145 G y Thr Cys Leu Asp u Val u 180 Ala Asp Ala Pro 195 His Arg Val Phe 210 Val His Leu Ala 225 Phe Asn Asp Ser Sy Leu Arg Ser 260 Asn Ala G u Asp 275 Val Ala G u Pro 290 Leu G y G u Thr 305 G y Hi s Hi s Cys Val Arg Ile Val 340 I le Al a Thr 5 Al a Val Leu Val Phe Asn Leu Tyr Hi s Asp 165 Asn Lys Pr o Lys Phe 245 Leu Ar g Su I e Ser 325 Val Al a Val Sy G u 70 Lys Val Hi s G u Al a 150 Thr SIle Leu Sy Thr 230 Ar g Ar g Pr o Sy Ar g 310 Lys Cys Asp Ser Leu 55 G u Leu Sy Ar g Al a 135 Leu Phe Pr o Ser G u 215 Sy Lys Tr p Ser Val 295 Val a n Asp Val Cys Leu 10 Al a G y Phe Pro His Ser 40 Thr Leu Asp Su G n Phe IVet Asn Arg 90 Thr Al a Thr 105 Al a Al a Asp 120 Leu Ala Trp Val G u Lys His Ile Leu 170 Al a G u Lys 185 IVet Asp Ile 200 G y Asp Phe Tyr Asp G y Al a u Val 250 Leu G u Asp 265 Al a Leu G u 280 Asp Phe Ile Leu Hi s G n Asp Tyr G n 330 Ar g G y Val 345 Ser Asp Al a Leu Leu Leu SIle Leu Sy Val 155 Ser SIle Leu Asp Pr o 235 Sy G n Leu G u Leu 315 Val Sy Sy G u Phe Lys Sy Asn Al a Lys 140 Asn Ar g Phe Ser Leu 220 I le Ar g Thr Ar g SIle 300 G y Tr p Thr Val G n G n Asn SIle Asp G u 125 Phe Hi s Sy Phe Tr p 205 Val Ser Thr Tr p Al a 285 Al a Phe Thr Al a I e Ar g Phe Hi s bet Asp Tyr Asn Al a G u 175 G n Ar g Phe Su I e 255 Al a Pr o Sy Leu Sy 335 Thr y Ala Pro Thr Thr 350 Page 470 seql i st cor r ect ed. t xt I e Ser Ala lMt y Phe Asp Thr 355 360 Pro Asp Pro u Ala Ala His Ala 365 Ar g Gy 385 Phe Phe Val Tyr Ser 465 Al a Asp Asp Leu Leu 545 Asp Gy Tr p Val G u Val Al a Val Phe 435 Al a SIle Ar g Leu Val 515 Val G n Asp Leu G u 595 Asp Leu Leu Pr o 405 Lys G n et Leu Leu 485 Al a Val G u Phe Asn 565 Phe Asn Ar g Lys 390 Ser G n Pr o Al a Val 470 Leu Al a Al a Asn Leu 550 Gy G u Al a Al a 375 Gy Pr o G u Tr p Leu 455 Ar g Ser Tyr G u Tyr 535 Asp G u Val Pr o Gy 615 G n Val Asp Al a G n 440 G u Asn Val Pr o Ar g 520 Tyr Thr Phe Val Val 600 Thr Tyr Gy Gy 425 Hi s Thr G n Al a G u 505 Al a Asp Leu Leu G u 585 Ar g Asp Pr o 395 Pr o SIle Asp Ar g bet 475 G u SIle Lys Val G u 555 Phe Ar g Pr o Sy Tr p Al a Al a 445 Asp Ser Gy Leu Sy 525 Al a Hi s Thr Sy SIe G u Pr o 415 Asp Leu Phe Asn G n 495 Thr Asp Phe Leu Thr 575 Al a Ala Ala n Tyr Arg u 605 Leu G u Ar g I Ie Pro Asn <210> 397 <211> 470 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> <223> RXN02839 <400> 397 tgt gtg gtg aat gat tat gct gac cgc aag ttt gat ggt cat gtt aag Cys Val Val Asn Asp Tyr Ala Asp Arg Lys Phe Asp y His Val Lys 1 5 10 Page 471 seql i st cor r ect ed. t xt cgc acg gcg aac cga Arg Thr Ala Asn Arg cca ctt ccc agc Pro Leu Pro Ser ggc gcg gta G y Al a Val aca gag aaa gag Thr G u Lys G u gcg cgc gcg ctg ttt gtc gtg ctg gta Al a Arg Al a Leu Phe Val Val Leu Val 40 ctg acg ctg aat acg atg acc att ctg Leu Thr Leu Asn Thr IVet Thr Ile Leu 55 ctg gcg tgg gtg tac ccg ttt atg aag Leu Ala Trp Val Tyr Pro Phe IVet Lys 70 gtg gtg ctg ggc gcg gcg ttt ggc tgg Val Val Leu Gy Ala Al a Phe y Trp gct gtg agt gag tcg gtg cca ttg agt Ala Val Ser u Ser Val Pro Leu Ser 100 105 aat att ctc tgg gcg gtg gct tac gac Asn Ile Leu Trp Ala Val Ala Tyr Asp 115 120 cgc gat gat gat gtg aag att ggc att Arg Asp Asp Asp Val Lys lie Gy lie 130 135 gcc aat acg ata aat tgatattggg atttt Ala Asn Thr Ile Asn 145 <210> 398 <211> 149 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 398 Cys Val Val Asn Asp Tyr Ala Asp Arg 1 5 Arg Thr Ala Asn Arg Pro Leu Pro Ser 25 Ala Arg Ala Leu Phe Val Val Leu Val 40 Leu Thr Leu Asn Thr IVet Thr Ile Leu Leu Ala Trp Val Tyr Pro Phe IVet Lys 70 Val Val Leu Gy Ala Al a Phe y Trp Ala Val Ser u Ser Val Pro Leu Ser 100 105 Asn Ile Leu Trp Ala Val Ala Tyr Asp 115 120 Arg Asp Asp Asp Val Lys lie Gy lie ctg att Leu II e ttg tcg Leu Ser cgg tat Arg Tyr tcg att Ser Ile 90 tgc tgg Cys Trp acg cag Thr G n aaa t cc Lys Ser tcg Ser at t I e acc Thr cca Pr o tta Leu t at Tyr acg Thr ttt Phe gcc Al a cat Hi s at g Met at g Met gcg Al a 125 gca Al a tta Leu gcg Al a ct a Leu gct Al a ttc Phe 110 at g Met at c l Ie ctg Leu ct a Leu ccg Pr o ttt Phe ct c Leu gtt Val ctg Leu tgcaga ttg Lys Phe 10 G y Al a Leu Il e Leu Ser Arg Tyr 75 Ser Ile I 90 Cys Trp I Thr G n Lys Ser Page 472 Sy Thr Phe Al a Hi s bet Met Al a 125 Al a Hi s G u Leu Al a Leu Al a Phe 110 Mlt lie Val Lys Leu Leu Pr o Phe Leu Val Leu Lys G u Val Al a G n Al a Al a Asp Leu seql i st cor r ect ed. t xt 130 135 Ala Asn Thr Ile Asn 145 <210> 399 <211> 978 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(955) <223> RXN00639 <400> 399 agtgttgtta tcgagttcag ccgatcacaa agatttttcc gcacccccta ct t caccccc aaagtctcta ggagtatgac 140 gctaggcagt gatccgactc cag G n aag Lys aac Asn aag Lys at c SIle gtt Val gga Gy aag Lys tgc Cys ggt Gy 150 t ac Tyr gtt Val aag Lys at c SIle gaa G u gtt Val cat Hi s aag Lys cct Pr o 120 cca Pr o gat Asp t cc Ser cca Pr o aac Asn gt a Val acc Thr gaa G u at c SIle t cc Ser gat Asp at c SIle gca Al a ttc Phe 170 aca Thr cgc Ar g gat Asp tac Ty r tac Ty r 75 gaa G u at c SIle gct Al a tt c Phe gaa G u 155 gcg Al a gcc Al a gtt Val ct g Leu gaa G u ggc G y gag G u gaa G u gaa G u gag G u 140 gtt Val cct Pr o cac Hi s t cc Ser ct c Leu 45 gag G u gag G u at c lie ggc Gy at g Met 125 ggg Gy gag gau gga Gy gat Asp t cc Ser gcg Al a t ac Tyr tgg Tr p aac Asn cct Pr o 110 cca Pr o cag G n ct c Leu at c SIle tcg Ser 15 gat Asp gcg Al a gca Al a cca Pr o t ac Tyr 95 t at Tyr at g Met gtt Val tgg Tr p cca Pr o 175 ggc Gy acc Thr at c l Ie gt g Val ct g Leu 80 aac Asn t ac Tyr cgt Ar g act Thr cac Hi s 160 gag G u at g Met 1 aac Asn t cc Ser gcc Al a ct c Leu tgg Tr p cgc Ar g gt a Val gac Asp gac Asp 145 gca Al a tgg Tr p aag Lys aag Lys cag G n aag Lys ttg Leu cac Hi s gag G u aag Lys 130 ct c Leu gat Asp aac Asn gca Al a gaa G u gtt Val cag G n gac Asp gac Asp aac Asn 115 gac Asp gac Asp gag G u ct g Leu act Thr cgc Ar g gct Al a tgg Tr p gtt Val t ac Tyr 100 tct Ser cgc Ar g ggc G y gac Asp cgt Ar g 180 act t ca gct Thr Ser Al a gaa Gu gac Asp gca Al a cac Hi s at g Met ttc Phe acc Thr ccg Pr o gca Al a aac Asn gga G y 165 ggc Gy acc atc gtt acc gat gag gaa ggc cgc tac aag atc aag acc ctg cag Page 473 Thr lie Val Thr Asp G u G u G 185 seql i st cor r ect ed. t xt y Arg Tyr Lys Ile Lys 190 Thr Leu G n 195 cct Pr o gag G u gtt Val 230 ggt Gy ct g Leu cca Pr o cct Pr o 200 t ac Tyr cac Hi s gag G u ct g Leu gt c Val 280 cag G n ggg Gy ggc Gy gt c Val 250 cct Pr o gat Asp cct Pr o cca Pr o 220 cgc Ar g aac Asn act Thr gaa G u gat ggc cca acc ggt tgg ttc Asp Gy Pro Thr Gy Trp Phe 210 cgc cca gcc cac ctc cac ttg Arg Pro Ala His Leu His Leu 225 atc acc acc cag ctt tac ttc Ile Thr Thr G n Leu Tyr Phe 240 gtt gca acc gct gtg aag cca Val Ala Thr Ala Val Lys Pro 255 260 gag gat ggt aac cac gtt cac G u Asp G y Asn His Val His 270 275 tagtttttct acctagctag cat <210> 400 <211> 285 <212> PRT <213> Corynebacter i um gl ut ai cur <400> 400 Met Thr Ser Ala G u n lie Val 1 5 Asn Lys Ala Thr Asp Lys Phe Lys Ser Lys u Arg Ala Asn Ala lie 40 Ala n Val Ala His Lys His u Leu Lys G n Trp IVet I e Asp Val 70 Trp Leu Asp Val Phe Val G u His Arg His Asp Tyr Thr G y Thr Lys 100 Val G u Asn Ser Pro Lys Leu Pro 115 120 Asp Lys Asp Arg Ala Cys Thr Pro 130 135 Asp Leu Asp G y Asn G y Leu Asp 145 150 Al a Asp G u Asp G y Tyr Tyr Ser 165 Asp Pro 10 Al a Asn 25 Tyr Val Val Thr G y G u G u lie 90 G y Ser 105 Trp Asp Leu II e G y Al a G n Phe 170 Thr Ala His Asp Ser G y Ar g Asp Tyr Tyr G u SIle Al a Phe G u 155 Al a Ser Leu G u G u SIle Sy bet 125 Sy G u Sy Ser Al a Tyr Tr p Asn Pr o 110 Pr o G n Leu SIle Asp Al a Al a Pr o Tyr Tyr bet Val Tr p Pr o 175 Trp Asn Leu Arg Oy Thr lie Val Thr Asp u Ou Oy Arg Tyr Lys Page 474 SIle Thr Hi s 225 G n Al a Asn Thr 195 Tr p Hi s Tyr Lys Val 275 G n SIle Ar g G u 245 G u Tyr Al a Ser 215 Ser Gy Val Phe seql i st cor r ect ed. t xt 185 Pro Tyr On lie Pro His 200 205 Tyr G y G y His Pro Trp 220 His Pro G y Tyr Arg Thr 235 G u Trp Val G u Asn Asp 250 Leu His Pro G u Thr G y 265 Val Leu Asp Lys G u Asp 280 285 G y Pro Pro Al a Thr Thr 240 Al a Thr 255 Asp y <210> 401 <211> 780 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(757) <223> RXN02530 <400> 401 gaggctgagc tttccgtcaa acaacatctt cccaatgtgg accccttcca aaccttgagt cccgtgatac aattgttgat atgaaatgac tgtgaccatc gaa G u gaa G u gat Asp ct c Leu at g Met t at Tyr gt c Val ttg Leu at c SIle t cc Ser aac Asn tta Leu cgc Ar g ggc Gy gt c Val gt g Val at t I e cgc Ar g ggg Gy aaa Lys gat Asp t ac Tyr cga Ar g agc Ser gca Al a ttc Phe ggt Gy cac Hi s aaa Lys aac Asn gag G u gat Asp cag gct gga G n Al a G y 15 ccc gga aaa Pro G y Lys gcc tgg cgg Al a Tr p Ar g at c gcc gat I e Al a Asp ccg gct t cc Pro Al a Ser 80 ttc tcc ttc Phe Ser Phe 95 act t cc gag Thr Ser G u 110 cga gag tgg Arg G u Trp atg t ca Vet Ser 1 ctc gcg Leu Al a gat ttt Asp Phe cat agg His Ar g ctc cca Leu Pro aca ttg Thr Leu gca gtt Al a Val gat cct Asp Pro aca aat tat Thr Asn Tyr gcg Al a ct c Leu tgg Tr p ggg Gy gtt Val gt g Val tcg Ser 115 gcg Al a gt c Val gat Asp ctt Leu gct Al a cgc Ar g 100 agc Ser cat Hi s ct c Leu t ca Ser ccc Pr o ggt Gy cca Pr o cca Pr o att acc cgc atg gtt Ile Thr Arg IVet Val Page 475 sec 120 125 ct t aat gca aca ggt acg t gg aca a Leu As n Ala Thr G31y Thr Tr p ThrA 135 140 ggc at c gat aaa t tc cag ggc aag c G3 y I I e As p Lys Phe G3 n G3 y Lys C 150 155 aag gcc gag gat t tc aaa ggt aag a Lys A a G3 u As p Phe Lys G3 y Lys L 1 70 t tg agt gct gt g caa t tt ct g ct g g Leu Ser Ala Val a31n Phe Leu Leu C 185 1 acc t gg gcg acg cgt cgt ccg cga a Thr Tr p Ala Thr Ar g Ar g Pr o Ar g T 200 205 cgg ct g ggg cat t gc ggt t gagcgcgc Ar g Leu a31y Hi s Cys a31y 215 <210> 402 <211> 219 <21 2> PRT <21 3> Cor ynebact er i urn gi ut anm cumn <400> 402 I'bt Ser Thr Asn Tyr a31u Ala I Ie I 1 5 Leu Al aAl aAl a Hi s a u Leu Ser P Asp Phe Leu Val Leu Asp Ser AsnA 40 Hi s Arg Trp Asp Ser Leu Thr Leu C Leu Pr o a31y Leu Pr o I'b t Asn Ar g F 70 Thr Leu Val Ala a31y Tyr Tyr a31y P Ala Val Val Ar g Pr o Val Lys Val S 100 1 Asp Pr o Ser Ser Pr o Leu Ar g Val S 115 120 Ilie Thr Ar g I'b t Val Leu Asn Ala T 130 135 Val Pr o Tyr Ilie Pr o a31y Ilie Asp L 145 150 Thr Val Asn Tyr Ar g Lys Ala a31 A 1 65 Val Val (3y (3y (3y Leu Ser Ala 180 1 lIi st cor r ect ed. t xt 130 .ac cct t at gt t ccg osn Pro Ty r Val Pr o 145 ag ct c cac acc gt t Sn Leu Hi s Thr Val 1 .aa gt c ct g gt c gt c .ys Val Leu Val Val 1 75 ag t tg gaa ggc tt g Su Leu a3 u a3 y Leu 90 Ct t ac gca gcg cga hr Tyr Ala Ala Ar g 210 c gt ccgcgaac gca t ac Tyr aat As n ggC a31y gcg A a 1 gt t ValI at t I Ie t ac Tyr ggt a31y 1 g aa a31u cga Ar g Ie lIe 10 ~rg Arg 25 Osp a3 y Sy Lys ~ro As p Il a Ty r 90 ier Ar g 05 ier Ser hr a3 y .ys Phe osp Phe 1 70 fal (31n 85 a3 y A a a3 y a3 n A a a3 y a31y Pr o A a Pr o a31u ValI As p Thr a31n 1 Lys5 Thr a31y a31y Thr a31u Pr o a31y 125 Thr Lys5 Lys5 Pr o A a I Ie Pr o Ph e Thr 110 Ar g As n a31n Lys5 a31y Tr p A a A a Ser Ser a31u Pr o Le u ValI 1 Phe Leu Leu (3 u Leu 03 u 1 Page 476 seqi i st car r ect ed. t xt Gy Leu Ala Olu Thr Thr Trp Ala Thr Arg Arg Pro Arg Thr Tyr Ala 195 200 205 Ala Arg Val Arg Arg Arg Leu G31y Hi s Cy s G31y 210 215 <210O> 403 <21 1> 990 <21 2> DNA <21 3> Cor ynebact er i urn gl ut ani cumn <220> <221 CDS <222> (967) <223> RXN00434 <400> 403 ccacgcgcca aaccat gcaa gccat cgt cc aaact gagga gaaggt cact gct t ctctgg aat tacagga agt ccccgt c ccgaccct ga agccaggt ag gt g ct g gt g aag gt g Val Leu Val Lys Val aag Ly s t at Tyr ggt G31y gt c ValI ccg Pr o gcg Al a at g vb t gcg Al a gt g ValI aag Ly s 1 50 gcc Al a gcg Al a gt g ValI at c I Ie t gc Cys ggt G31y t cg Ser gcg Al a ggt G31y 120 gt t ValI tt a Le u gt t ValI gt t ValI gcg Al a aat As n ct c Le u tt g Le u gt g ValI tt g Le u gga G31y gt g ValI gcc Al a aag Ly s 1 70 cgt Ar g gct Al a ggc G31y G31y cca Pr o gt t ValI aag Ly s tt t Ph e gcc Al a 140 at c I Ie aag Ly s gcg Al a t cg Ser g ac As p 45 ggt G31y at t I Ie gcg Al a gag O31u gcc Al a 125 ggt G31y ct c Le u gcg Al a g at As p gag O31u act Thr gga G31y cca Pr o t gc Cys g at As p 110 at t I Ie t ca Ser at c I Ie g at As p tt g Le u ct c Le u c aa O31n gcg Al a ggt G31y 80 gt g ValI tt c Ph e at g vb t g aa O31u t ac Tyr 1 60 at t I Ie 1 c ag O31n G31y ac a Thr c aa O31n 65 t ac Tyr t gg Tr p ct t Le u ggc G31y aag Lys5 145 aag Lys5 ct c Le u ac g Thr ct g Le u gt g ValI 50 t at Tyr ag c Ser t cg Ser at t I Ie aag Lys5 130 tt a Le u gag (31u g at As p c ag O31n g aa O31u 35 ggt O31y gt g ValI tt t Ph e aat As n cat Hi s 115 gct Al a aaa Lys5 g aa O31u at t I Ie gga O31y 20 t gt Cys c ag O31n gcg Al a gt g ValI at c I Ie 1 00 ggt O31y ct g Le u ac c Thr g at As p at t I Ie 1 aat As n gcg Al a g aa O31u gt t ValI g aa O31u ggc O31y 99c O31y ggt O31y t gt Cys tt c Ph e 1 ggt (31y Page 477 gcg aag tat ttg tca cag aat Ala Lys Tyr Leu Ser G n Asn 185 atg gta gtc atc ggg atg cag Mbt Val Val Ile y Mbt n 200 ggt cat ctt ttg gcc aag cga G y His Leu Leu Ala Lys Arg 215 220 ggt cgc gat gag gcg gat aag G y Arg Asp G u Ala Asp Lys 230 235 aat att tgg ccg ctg ctg caa Asn lie Trp Pro Leu Leu G n 250 cac acc ttg ccg cta gcc gaa His Thr Leu Pro Leu Al a G u 265 gac ggc acc atc acc ggc aag Asp y Thr Ile Thr y Lys 280 atgccagcac cct <210> 404 <211> 289 <212> PRT <213> Cor ynebact er i um gl ut ar seql i st cor r ect ed. t xt gtg aag gcg atg gcc aag gac gcg Val Lys Ala IVet Ala Lys Asp Ala 190 195 ggt ggc gtg aaa ggg gag ctg aat G y y Val Lys y u Leu Asn 205 210 ggc acg att tct gcc act gcg ctg Sy Thr lie Ser Ala Thr Ala Leu 225 gct cgg att gtc agc agc act gtc Ala Arg lie Val Ser Ser Thr Val 240 tcg aag gaa att acc cct cac atc Ser Lys u Ile Thr Pro His Ile 255 260 gca gcc gcc gcc ttg cag aaa att Ala Ala Ala Ala Leu G n Lys Ile 270 275 ctc gtg ctt gcg gtt taggcaagcg Leu Val Leu Ala Val 285 cac Hi s ttg Leu cgt Ar g gaa G u 245 gac Asp caa G n <400> 404 Val Leu Val Lys Val Lys Pro Ala y Val Asn J 1 G n Sy Thr G n Tyr Tr p Leu Sy Lys 145 G n G u Sy Val Phe Asn Hi s 115 Al a Lys 5 Asn Al a G u Val G u Sy Sy Sy Cys Tyr Sy Val Pr o 70 Al a Met Al a Val Lys 150 Pr o Val Al a G u Al a Leu Sy Thr 135 Asn Pr o Val Leu G n l Ie Sy 105 I le Al a Sy 10 Al a G y Asn Al a Leu Thr Leu Met 75 Val G u 90 Leu G n Sy Thr Val Thr Al a Asp 155 Page 478 Ar g Al a Sy Sy Pr o Val Lys Phe Al a 140 I le Al a Ser Asp Sy SI e Al a G u Al a 125 Sy Leu Leu I e Sy Tyr G u Thr Thr G n Thr Asn Leu Leu G n Al a Sy Val Phe bet G u Tyr 160 Lys Leu Al a Sy Al a 225 Ser Thr Leu Val Su I e Asp 195 Leu Al a Thr Hi s Lys 275 Phe 165 Gy Hi s Leu Ar g G u 245 Asp G n Al a Al a Met Gy Gy 230 Asn Hi s Asp G u Lys Val Hi s 215 Ar g SIle Thr Gy seql i st cor r ect ed. t xt Val Leu Lys Asn Lys Ala 170 Tyr Leu Ser G n Asn Val 185 Val Ile y 'e t Gn y 200 205 Leu Leu Ala Lys Arg y 220 Asp G u Ala Asp Lys Ala 235 Trp Pro Leu Leu G n Ser 250 Leu Pro Leu Ala G u Ala 265 Thr Ile Thr y Lys Leu 280 285 Asp Lys 190 Gy Thr Ar g Lys Al a 270 Val Val 175 Al a Val SIe SIe G u 255 Al a Leu <210> 405 <211> 1098 <212> DNA <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(1075) <223> RXN01619 <400> 405 cctgcaagtt tactgctcgg ccgtcacggg ggaatggaaa tagcgaaccc attttctatt gcgatgagag gaacaccacc cac Hi s gaa G u gca Al a ttc Phe gt c Val gtt Val act Thr ccc Pr o at c l Ie cca Pr o gcc Al a t cc Ser ggc G y ccc Pr o aac Asn ct g Leu ggc Gy acc Thr ccc Pr o cca Pr o gat Asp gcc Al a ggc Gy ttt Phe gcc Al a ggt G y ttg Leu 45 gcc Al a gt c Val ggc Gy gac Asp cag G n tgg Tr p gca Al a gaa Gu at c SIle gt c ct a Val Leu 15 gtt cgt Val Arg acc gtg Thr Val ggc acc G y Thr ggt ttg G y Leu 80 tgg gcg Trp Al a 95 Page 479 aagtacgctt ggtgt t cata atg cgc gca atc act 115 IVet Arg Ala lie Thr 1 cag att acc gag aag 163 G n Ile Thr G u Lys att caa gtg acg ctg 211 lie G n Val Thr Leu aag ggc tct tac ggc 259 Lys G y Ser Tyr G y gag gca gtc ggc atc 307 Su Ala Val Gy Ile cag gtc ggt cag cgt 355 Sn Val y Gn Arg gag tac gcg ctt gtc 403 G u Tyr Ala Leu Val 100 seql i st cor r ect ed. t xt gac Asp gca Al a ttc Phe ggt Sy 150 cat Hi s gct Al a aag Lys gat Asp gaa G u 230 gaa G u ttc Phe cag G n ctt Leu cgc Ar g 310 gcc tcc ggc Al a Ser G y 105 gct cag ct c Al a G n Leu 120 ctg gat atg Leu Asp IVet 135 gcc gtc ggc Ala Val G y gtc gtt ggt Val Val G y caa aac at c G n Asn Ile 185 cag gtc gaa Sn Val u 200 t cc gtc ggt Ser Val G y 215 ggc ggc acc G y G y Thr at c cca t cc Ile Pro Ser tgg gga agc Trp G y Ser 265 ttg ttc ggc Leu Phe G y 280 cca gtt gat Pro Val Asp 295 gcc tcc agc Al a Ser Ser ctc att ccc gta Leu Ile Pro Val cca gaa cag ctc t cc G n Leu Ser gt c Val aaa Lys cgc Ar g ct c Leu 170 agc Ser gac Asp gga Gy ct c Leu ggc Gy 250 aaa Lys gag G u t cc Ser gag G u gca Al a cca Pr o at g bet 155 gt c Val ggc G y at c SIle t cc Ser gt c Val 235 ccc Pr o gt c Val ct c Leu acc Thr cct Pr o 315 at g Met ggg G y 140 ct c Leu cgc Ar g gt c Val acc Thr t cc Ser 220 t cc Ser gt c Val agc Ser at t SIle ttt Phe 300 ggc G y cct Pr o 125 gag G u gca Al a cgt Ar g gtt Val ggt Gy 205 gca Al a ttc Phe at c SIle cgc Ar g gcg Al a 285 gat Asp cgt Ar g Pr o 110 ttc Phe tgg Tr p cag G n gac Asp t cc Ser 190 ggc Gy gct Al a ggc Gy ttc Phe gaa G u 270 cgc Ar g gcc Al a gcc Al a G u agc Ser ct g Leu ct g Leu gcc Al a 175 act Thr gca Al a gac Asp gcc Al a aag Lys 255 at g Met at a SIle gct Al a gga G y gcc Al a at c SIle gca Al a 160 ggt G y gag G u agc Ser ct g Leu at g Met 240 cac Hi s cca Pr o ctt Leu gac Asp aaa Lys 320 at c l Ie caa G n 145 gaa G u gt c Val acc Thr at c SIle gt g Val 225 ggc Gy at c SIle gca Al a gat Asp at c SIle 305 gt g Val agc Ser 130 aac Asn t cc Ser caa G n cca Pr o gcc Al a 210 aaa Lys aac Asn acc Thr gag G u gga G y 290 gt c Val ct c Leu gaa G u ctt Leu gca Al a ggc G y ct c Leu 180 tgg Tr p gca Al a ctt Leu at c SIe aag Lys 260 aaa Lys ttg Leu gcc Al a cgt Ar g 451 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1098 taaacgttta aggcccatta gac <210> 406 <211> 325 <212> PRT <213> Corynebacteri um gl ut am cum <400> 406 Iet Arg Ala lie Thr His Asn Thr Phe G y Asp Pro Ala Asp Val Leu 1 5 10 Page 480 seqI i st car r ect ed. t xt On lieThr G u Lys G u Ilie Pro Thr 2 Pro G31y Pr o 31y O31n Val I Ie Ly s O31u O31n O31u Le u I Ie G n 145 O31u ValI Thr I Ie ValI 225 Gy le A a As p I Ie 305 ValI ValI Ser ValI G31y A a As p 115 Le u Ser Ar g O31u G31y 1 95 ValI Le u Pr o ValI Ly s 275 Thr Ser I Ie Le u G31y I Ie Ar g ValI Ser As p As n I Ie 1 65 A a O31u Le u G31y vb t 245 G31y Thr Thr ValI Ph e 325 A a Ph e ValI 70 ValI As p A a Ph e G y 150 Hi s A a Ly s As p 0Ou 230 0Ou Ph e O n Le u Ar g 310 Thr ValI As p A a A a A a Le u 135 A a ValI O31n O31n Ser 215 O31y I Ie Tr p Le u Pr o 295 A a Hi s As p Le u O31y O31y 1 05 Le u vb t G y Oy le 1 85 O31u O31y Thr Ser Ser 265 O31y As p Ser Hi s Pr o O31u 75 Ser I Ie A a Pr o vb t 1 55 ValI Oy le Ser ValI 235 Pr a ValI Le u Thr Pr a 315 Le u A a ValI O31y ValI Pr a 125 O31u A a Ar g ValI O31y 205 A a Ph e I Ie Ar g A a 285 As p Ar g Tr p A a O31u I Ie Pr a 110 Ph e Tr p G n As p Ser 1 O31y A a O31y Ph e O31u 270 Ar g A a A a Thr O31y O31y Tr p O31u Ser Le u Le u A a 1 Thr A a As p A a Ly s 255 vb t I Ie A a (31y Ar g ValI Thr Le u A a O31n A a I Ie A a 1 O31y O31u Ser Le u vb t 240 Hi s Pr a Le u As p Ly s 320 <210O> 407 <21 1> 1041 <212> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> Page 481 seal i st cor r ect ed. t xt <221> CDS <222> (101)..(1018) <223> RXN01842 <400> 407 cacgtcatgg t ctcgcggtg at catct t cc taccgtgaca gg tgaccacagg aaagaaattc acgaggagag gaagcacacg at caaggccg caaacggccg g gt g Val aac Asn gcc Al a acc Thr gt c Val gt g Val ct g Leu acc Thr act Thr gcc Al a 150 aag Lys gaa G u cag G n gt c Val aac Asn ccc Pr o gt c Val cga Ar g gcc Al a ggc G y gt g Val gcc Al a 120 cag G n ggt Gy cag G n acc Thr cgt Ar g 200 ggg Gy t ac Tyr cca Pr o ccg Pr o ctt Leu gga G y gt g Val gag G u gcg Al a gag G u gt c Val gt c Val 170 gct Al a ct g Leu gcg Al a ggc Gy ccg Pr o gat Asp gca Al a ggt G y ccc Pr o acc Thr ccg Pr o gaa G u 140 ct g Leu gtt Val ttc Phe cct Pr o cgg Ar g 220 ccg Pr o gga Gy tgg Tr p 45 ccc Pr o gt g Val ggc G y gt g Val gt c Val 125 cca Pr o at g Met gac Asp gt c Val gac Asp 205 gt g Val gaa G u gaa G u aag Lys ct g Leu gag G u gt c Val ct a Leu 110 gct Al a ggc G y gcc Al a cac Hi s gcc Al a 190 ggt Gy gtt Val aac Asn 15 ct c Leu gt c Val ggc Gy ggc G y ggc G y 95 aag Lys ggg G y cag G n gcg Al a gag G u 175 acc Thr gt g Val gct Al a cag G n ggg G y cgt Ar g gag G u ttc Phe 80 gga G y ccg Pr o gcg Al a tcg Ser cag G n 160 gac Asp ggc G y gac Asp ccc Pr o Met 1 gaa G u gt c Val t cc Ser gag G u gcg Al a t at Tyr gag G u agc Ser ttg Leu 145 at c SIle aag Lys gac Asp gt a Val tta Leu 225 tcg aag gta tac Ser Lys Val Tyr ctg atc acc cgc Leu Ile Thr Arg aag gtc cac gcg Lys Val His Ala ggg gtt gcc gga Sy Val Ala y gcc tcc ggg atc Al a Ser G y Ile gtc ggc gat ccg Val y Asp Pro gcc gag gac acc Al a G u Asp Thr 100 gag atc tcg ttc G u Ile Ser Phe 115 gcc tac gcc ggc Al a Tyr Al a y 130 ctg atc aat ggg Leu Ile Asn G y gga cgg gtc cac G y Arg Val His 165 cgc gag ctc atc Arg u Leu Ile 180 gcc gtc gcg gag Ala Val Ala G u 195 gtc ttc gac cta Val Phe Asp Leu 210 gcg aag aat ccg Ala Lys Asn Pro gcg cac gtg atc tcg gcg gct gat gct gcc acc gtg gga gaa ctc ggt Page 482 seqI i st car r ect ed. t xt Ala His Val IlieSer Al a Al a Asp Al a Al a Thr Val (31y 230 235 240 gga cag gtg ctg cgc cgc acc ccg gaa atg gtc gga cag G31y 1n ValI Leu Ar gAr gThr Pr o (1uIb t Val O31y (1n 250 255 gtg gtc cag tac ggg ct g gtc gac ccg aag gtc gat acg Val Val O31n Tyr O31y Leu Val Asp Pr o Lys Val Asp Thr 265 270 ct g gaa cag gcc ggt aag gcc ct g gcc cac gt t gag cag Leu (1u (1n Al a (1y Lys AiaLeu AliaHi sVali3 Gu (1n 280 285 290 cgc ggc aag at c gtc cCtc gag ct c at c acc t cc cag gac Ar g G31y Lys Ilie Val Leu O31u Leu Ilie Thr Ser O31n Asp 295 300 305 acgcggt gac ct C <210> 408 <211> 306 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ami cumn O3 u Leu 03 y 245 at c acc ggg Il e Thr 03 y 260 acc t ac ccg Thr Tyr Pr o 275 ggc cac gcc O3 y Hi s Al a t aaccagaca 883 931 979 1 028 1041 <400> 408 I'bt Ser Lys Val Tyr Val Ser Asn 0 u O31u ValI Ser O31u Al a Tyr O31u Ser Le u 145 li1e As p I Ie ValI ValI Ser O31y O31u I Ie 115 Tyr I Ie Ar g O31u ValI 1 95 Ar g Al a O31y I Ie Pr o Thr Ph e O31y O31y Hi s 1 65 li1e O31u Thr O31y Pr o Thr Le u Le u As p Hi s 135 G31y Ph e Ser ValI O31n As n O31u ValI Le u Al a 1 05 Al a ValI G31y ValI G31y 1 85 Al a Tyr 10 Pr o Pr o Le u G31y ValI 90 O31u Al a O31u ValI ValI 1 70 Al a Le u G31y G31y Le u Pr a As p Al a Ser I Ie Le u G31y 1 Gy le Le u Pr a G31y Tr p Pr a ValI G31y ValI ValI 125 Pr a vb t As p ValI As p 205 O31u O31u Lys5 Le u O31u ValI Le u 110 Al a G31y Al a Hi s Al a 1 G31y As n Le u ValI G31y G31y G31y G31y O31n Al a O31u 1 Th r ValI Val Val Phe Asp Leu Val Oy Oy Ou Ala Leu Arg Val Val Ala Pro Page 483 seql i st cor r ec 210 215 Leu Ala Lys Asn Pro Ala His Val lie Ser Ala 225 230 235 Val G y G u Leu G y G y G n Val Leu Arg Arg 245 250 Gy n Ile Thr y Val Val n Tyr y Leu 260 265 Asp Thr Thr Tyr Pro Leu G u n Ala y Lys 275 280 G u On y His Ala Arg y Lys lie Val Leu 290 295 Sn Asp 305 <210> 409 <211> 1614 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(1591) <223> RXN00641 <400> 409 tgcggaattg ctcgcaaatg tcacacaccg cttcaaagca gtggcaatac caacttcttt tcactctctt ggaggttcac ted. txt 220 Al a Asp Al a Al a Thr 240 Thr Pro G u Mbt Val 255 Val Asp Pro Lys Val 270 Ala Leu Ala His Val 285 G u Leu Ile Thr Ser 300 aaaacgaaaa cgacatcgcg t ca Ser cgc Ar g cct Pr o at g Met acc Thr ggc Gy ct c Leu cac Hi s ttg Leu gaa G u at c SIle at g Met t ac Tyr ct c Leu cgt Ar g tgg Tr p agc Ser gga Gy gag G u cac Hi s ggc Gy ggc Gy aag Lys ttc Phe cag G n gt c Val gag G u t cc Ser cag G n at c SIle gac Asp aac Asn act ct g Thr Leu 15 gtc aac Val Asn cgc cac Arg Hi s at c cct Ile Pro at c at g I e bet gcg tgt Al a Cys 95 cgc acc Arg Thr 110 ggc gca G y Al a gac Asp cgc Ar g at c SIle aac Asn at c SI e 80 tct Ser t cc Ser atg t cc Vet Ser 1 cat gcg Hi s Al a aac at c Asn Il e ttc gaa Phe G u ggt gga G y G y acc cgt Thr Arg cac cgt His Ar g ttg acc Leu Thr aca cca gtt Thr Pro Val ctt gag gac Leu G u Asp ttc act gac Phe Thr Asp ggc atc t gg Sy lle Trp gac tac ttc Asp Tyr Phe tcc aag gaa Ser Lys G u ggc gcc atg G y Al a Vet 100 tgc cca ttc Cys Pro Phe 115 ctg ctc aag gtc aag ggc Leu Leu Lys Val Lys G y Page 484 seql i st cor r ect ed. t xt gaa Gu gat Asp 150 ggc Gy acc Thr gaa G u ct c Leu tgg Tr p 230 aac Asn gga Gy ggc Gy aag Lys aat Asn 310 cag G n at c SIle cgc Ar g cca Pr o t ct Ser gaa Gu cgt Ar g ct c Leu acc Thr ct g Leu 200 acc Thr t ac Tyr acc Thr ttc Phe cca Pr o 280 ttc Phe tgc Cys cgc Ar g tgc Cys cgc Ar g 360 gac Asp ttc Phe gcc Al a gtt Val gat Asp 170 at t SIle gga Gy aac Asn gca Al a gca Al a 250 t ac Tyr gac Asp gaa Gu t ac Tyr at c SIle 330 gca Al a t ac Tyr gag G u tgg Tr p tac Tyr cct Pr o 155 gat Asp gac Asp t cc Ser ggt Gy acc Thr 235 at g Met cct Pr o cgc Ar g gaa Gu ccc Pr o 315 cgc Ar g cct Pr o gaa G u gaa G u aat Asn cca Pr o 140 aag Lys gt c Val at g Met t cc Ser gca Al a 220 acc Thr gac Asp t ac Tyr cca Pr o aag Lys 300 aat Asn cca Pr o aag Lys gac Asp ttc Phe 380 gac Asp gag Gu tta Leu gtt Val ct g Leu acc Thr 205 gac Asp t cc Ser gct Al a ggc Gy ct g Leu 285 ggc Gy gtt Val at c SIle ggc Gy ttc Phe 365 cgc Ar g at g let aat ttc cgc Asn Phe Arg gag t cc tac G u Ser Tyr 160 tct ttg gaa Ser Leu G u 175 gtt gac caa Val Asp G n 190 t ac acc t ac Tyr Thr Tyr ggc tac cac G y Tyr Hi s cgc cgt ggc Arg Arg G y 240 ggt acc tgg G y Thr Trp 255 cac at g ct g His Vet Leu 270 ttc gag cgc Phe G u Ar g gag ttc atg G u Phe IVet tac ctg at g Tyr Leu IVet 320 t ca gt t gat Ser Val Asp 335 gag tcc gcg G u Ser Al a 350 ttc aac gca Phe Asn Al a t cc tgc cag Ser Cys G n acc cgc ggt Thr Arg G y gac Asp ggc Gy cac Hi s cca Pr o ggc Gy 210 t cc Ser ggt Gy aag Lys tgg Tr p gac Asp 290 ggt Gy cag G n acc Thr gca Al a ggc Gy 370 acc Thr ggc Gy ttc Phe ct c Leu gaa G u 195 aac Asn t cc Ser gaa G u cag G n at g Met 275 gag G u gct Al a ttc Phe gaa G u cgt Ar g 355 at g Met t ac Tyr t cc Ser ct c Leu ggc Gy 180 ggc Gy tgg Tr p acc Thr t cc Ser ggt Gy 260 tgg Tr p ttc Phe t cc Ser t cc Ser gt c Val 340 gca Al a gca Al a cag G n cac Hi s ttc Phe 165 gac Asp ct c Leu aag Lys cac Hi s gcg Al a 245 ggc Gy tgg Tr p aag Lys cgc Ar g t ca Ser 325 acc Thr aac Asn acc Thr gca Al a 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1171 1219 1267 1315 ttg ggc cac cag gta Leu G y His G n Val Page 485 390 395 cag gga cca aac gag gt t gcc O31n G31y Pro Asn O31u Val Ala 410 tcc tcc gga gca cgc acc gaa Ser Ser G31y Ala Ar g Thr O31u 425 ggc t tc t gg cat gaa ct c at g G3 y Phe Tr p Hi s G3 u Leu I'b t 440 aag gaa aag gaa ttg gct gac Lys 03 u Lys 03 u Leu A a Asp 455 460 gct gca gcc aaa at c cgt gag Ala Ala Ala Lys Ilie Ar g O31u 470 475 gct gga gag cct cgc cgc cgt Ala G31y 31u Pr o Ar g Ar g Ar g 490 aaagcaaaaa at c <210> 410 <211> 497 <21 2> PRT <21 3> Cor ynebact er i urn gi ut ar seqI i st car r ect ed. t xt 400 aag ggc cta ggc atg aac gaa gtt Lys 03 y Leu 03 y I'b t Asn 03 u Val 415 420 gat gaa ggc ctc tac cca atc cag Asp O31u G31y Leu Tyr Pr o Ilie O31n 430 435 cag gag gct gt g aat aag cag agc O31n O31u Ala Val Asn Lys O31n Ser 445 450 gat acc gct t ct t cc ct t gcc acc Asp Thr A a Ser Ser Leu A a Thr 465 gaa gca aag gca gcc gcg aag t cc O3 u A a Lys A a A a A a Lys Ser 480 cgt cgc acc cgc ggt t agt cgt cga Ar g Ar g Thr Ar g O31y 495 405 ct t Le u c ac Hi s at c I Ie gt a ValI g ac As p 485 1363 1411 1459 1 507 1 555 1601 1614 <400> 410 I'bt Ser Thr Hi s A a Leu As n I I e Phe Phe 03 u 03 y O31y 1y As p Thr Ar g Ser Hi s Ar g (31y Leu Thr Cys 115 Leu Lys Val 130 Thr Asp 03 y 145 Ar g O31y Phe Pr o Val Ser O31u Asp Ar g Thr As p Pr o I Ie Tr p I'b t Ty r Phe Thr 70 Lys 03 u 03 y Ala I'b t Leu 1 00 Pr o Phe Hi s Lys 03 y 03 u Ser Hi s As p 150 Leu Phe 03 y 1 65 As n Pr o O31u As p Thr Thr Cys O31y Lys5 135 ValI Ser A a O31u Ph e A a I Ie As n O31y 1 Pr o O31y Ar g As n Ser 10 O31y O31u Hi s O31y O31y 90 Lys5 Ph e A a ValI As p 1 70 ValI I Ie Le u O31u Cys Le u Ser Cys Tyr Pr o 1 Lys5 Ar g vb t O31n Ar g As n As n O31y 125 O31u Le u Le u As n Hi s Pr a vb t Cys Th r A a Ph e Se r Asp Val Val Ser Leu O31u 1 Page 486 G u His Leu G y Asp 180 seql i st cor r ect ed. t xt Thr Arg Thr Val lie Asp IVet Leu Val Asp G n 190 Ser Asp Val 225 Thr Sy Leu Ar g Val 305 Asp G n G u Thr Lys 385 Leu bet Tyr Asn Ser 465 Al a Gy G u 195 Asn Ser G u G n let 275 G u Al a Phe G u Ar g 355 let Tyr Hi s G u SIle 435 G n Al a Lys Leu Lys Hi s Al a 245 Sy Tr p Lys Ar g Ser 325 Thr Asn Thr Al a Val 405 Leu Hi s SIle Val Asp 485 G u Leu Tr p 230 Asn Sy Sy Lys Asn 310 G n SIle Ar g Pr o Ser 390 G n Ser Sy Lys Al a 470 Al a Val G n 215 Asn G u Tyr Asn G u 295 Leu SIle Tyr SIle Asp 375 Al a Sy Ser Phe G u 455 Al a Sy Leu 200 Thr Tyr Thr Phe Pr o 280 Phe Cys Ar g Cys Ar g 360 Asp Phe Pr o Sy Tr p 440 Lys Al a G u 185 Ar g G u Al a Lys Ser 265 G u Sy Leu Hi s SIle 345 G n Leu Pr o Asn Al a 425 Hi s G u Lys Pr o Sy Asn Al a Al a 250 Tyr Asp G u Tyr SIle 330 Al a Tyr G u Tr p G u 410 Ar g G u Leu SIle Ar g 490 Ser Sy Thr 235 let Pr o Ar g G u Pr o 315 Ar g Pr o G u G u Asn 395 Val Thr Leu Al a Ar g 475 Ar g Thr 205 Asp Ser Al a Sy Leu 285 Sy Val SIle Sy Phe 365 Ar g Met Lys Asp G n 445 Asp G u Ar g Tyr Sy Ar g Sy Hi s 270 Phe G u Tyr Ser G u 350 Phe Ser Thr Sy G u 430 G u Thr Al a Ar g Thr Tyr Ar g Thr 255 bet G u Phe Leu Val 335 Ser Asn Cys Ar g Leu 415 G y Al a Al a Lys Thr 495 Tyr Hi s Sy 240 Tr p Leu Ar g Met let 320 Asp Al a Al a G n Sy 400 Sy Leu Val Ser Al a 480 Ar g <210> 411 <211> 1166 <212> DNA <213> Corynebacter i um gl utam cum Page 487 seql i st cor r ect ed. t xt <220> <221> CDS <222> (16)..(1143) <223> RXN01993 <400> 411 ctttaggagt tcacc atg aca ctg tcc gaa cgc aag ctc acc acc acc gcc IVet Thr Leu Ser G u Arg Lys Leu Thr Thr Thr Ala 1 5 aag Lys t at Tyr cca Pr o gac Asp ggc G y gcg Al a gca Al a gt g Val 125 at g Met t cc Ser acc Thr agt Ser acc Thr 205 at g Met ctt Leu gt c Val gct Al a tgc Cys gac Asp cgc Ar g gt c Val ct g Leu cag G n gac Asp gaa G u 175 t ca Ser tgg Tr p gat Asp cac cca ctc aac Hi s tct Ser t ac Tyr cac Hi s at c SIle at g Met t cc Ser gat Asp agc Ser 145 aac Asn ttc Phe ttc Phe cat Hi s ttc Phe 225 Pr o aaa Lys cgc Ar g 50 cgc Ar g caa G n aaa Lys tac Ty r ccc Pr o 130 cac Hi s tac Ty r gt g Val gt g Val gac Asp 210 cca Pr o Leu aag Lys acc Thr ct c Leu tgc Cys at g Met ccc Pr o 115 aca Thr ccc Pr o caa G n cac Hi s gt c Val 195 at a SIle gga Gy Asn 20 ccc Pr o aaa Lys gca Al a cct Pr o ccc Pr o 100 gt g Val ttg Leu gaa G u tta Leu t cc Ser 180 acc Thr gat Asp ttt Phe gcc Al a at g let gat Asp ccg Pr o t at Tyr gcg Al a gt g Val gca Al a tgg Tr p gt g Val 165 t cc Ser cac Hi s gca Al a gaa G u tgg Tr p gcc Al a ggc Gy ct a Leu 70 cac Hi s cag G n gaa G u gat Asp gca Al a 150 ct g Leu agc Ser act Thr cca Pr o ggc 30y 230 tac gt c Tyr Val agg aca Arg Thr cga gcc Arg Ala 55 t cc aag Ser Lys ggc ttg G y Leu gaa acc G u Thr gcc cac Al a Hi s 120 ccc acc Pro Thr 135 ggc gat G y Asp gac aac Asp Asn atc gga I e G y gaa gat Su Asp 200 ccg ttt Pro Phe 215 aag gt g Lys Val gcc Al a at c SIle gtt Val gga G y gag G u ct c Leu 105 cgc Ar g caa G n gga G y ttg Leu caa G n 185 t cc Ser tgg Tr p gat Asp gct Al a gcc Al a gcc Al a aaa Lys t ac Tyr aac Asn ttt Phe gt a Val cgc Ar g at g Met 170 gac Asp gt g Val caa G n cgt Ar g tgg Tr p aac Asn ctt Leu ct c Leu aac Asn ccg Pr o gt g Val ccc Pr o acc Thr 155 gac Asp gaa G u acg Thr aag Lys tgg Tr p 235 gat Asp aaa Lys gca Al a gtg Val t cc Ser t ca Ser tgg Tr p gat Asp 140 at c SIe ct c Leu ctt Leu gtc Val aac Asn 220 cag G n atc atc cac tac tac tac cct tcc acc atc tgc att gat gtt ggt gta Page 488 Il e Il e Hi s seql i st cor r ect ed. t xt Tyr Tyr Pro Ser Thr Ile Cys Ile Asp Val G y Val 250 gca aag gct gga Ala Lys Al a G y 255 aat ggg tat gtc Asn G y Tyr Val 270 cat tac ttc tgg His Tyr Phe Trp 285 atc acc acc cag Ile Thr Thr G n gaa gac atg ctg G u Asp IVet Leu 320 tac gag ttt tac Tyr G u Phe Tyr 335 cga atc ctc gag Arg lie Leu u 350 acc aca ttc ccc Thr Thr Phe Pro 365 actcgcaatg gca <210> 412 <211> 376 <212> PRT <213> Corynebact <400> 412 Met Thr Leu Ser 1 His Pro Leu Asn Ser Lys Lys Pro Tyr Arg Thr Lys His Arg Leu Ala Ile n Cys Pro Met Lys Met Pro 100 Ser Tyr Pro Val 115 act Thr at g Met gca Al a ct g Leu 305 acc Thr agc Ser gaa G u cgc Ar g ggc G y aac Asn tt c Phe 290 cgc Ar g gct Al a ct c Leu gca Al a gca Al a 370 gcg Al a acc Thr 275 at g Met gac Asp cag G n aac Asn ct c Leu 355 aca Thr cag G n 260 at t SIle cgc Ar g ggt G y caa G n at t SIle 340 t cc Ser ccg Pr o 245 gaa G u acc Thr aac Asn gt a Val gat Asp 325 gat Asp aag Lys aag Lys ggc G y cca Pr o t ac Tyr t cc Ser 310 gcc Al a gcc Al a gaa G u ccg Pr o gac Asp gat Asp cgc Ar g 295 ggt G y at c l Ie ggt G y ggc G y gag G u 375 cgc Ar g t ca Ser 280 ct g Leu gt a Val gac Asp ggc G y cga Ar g 360 gca Al a Al a Asp Lys Al a Val Ser Ser Tr p agc cag ggc Ser G n G y 265 gat cgt t cc Asp Arg Ser gaa agc caa Su Ser G n ttc aaa gaa Phe Lys G u 315 gcc aac acc Al a Asn Thr 330 atg tgg gt g Vet Trp Val 345 ctg gat at c Leu Asp Ile t aaaccat ga 819 867 915 963 1011 1059 1107 1153 1166 er i um gl ut ai cum G u Arg Lys Leu Thr 5 Ala Trp Tyr Val Ala 25 IVet Ala Arg Thr Ile 40 Asp G y Arg Ala Val 55 Pro Leu Ser Lys y 70 Tyr His G y Leu G u Ala G n G u Thr Leu 105 Val G u Ala His Arg 120 Thr Thr 10 Al a Tr p Al a Asn Al a Leu Lys Leu Tyr Asn 90 Asn Pro Phe Val Page 489 Lys Tyr Pr o Asp Sy Al a Al a Val 125 SIle G u Leu Al a Thr Sy Al a 110 Tr p Leu Val Al a Cys Asp Ar g Val Leu Asp Pro Thr Leu Ala Asp Pro Th 130 135 seql i st cor r ect ed. t xt r G n Val Pro Asp Mbet 140 His G n Met Ser 145 Asn Phe Phe Hi s Phe 225 Tyr Thr Met Al a Leu 305 Thr Ser G u Ar g Pr o G n Hi s Val 195 I le Sy Pr o Al a Thr 275 Met Asp G n Asn Leu 355 Thr Tr p Val 165 Ser Hi s Al a G u Thr 245 G u Thr Asn Val Asp 325 Asp Lys Al a 150 Leu Ser Thr Pr o Gy 230 I le Gy Pr o Tyr Ser 310 Al a Al a G u Sy Asp SIle G u Pr o 215 Lys Cys Asp Asp Ar g 295 Gy lie Gy Gy Gy Leu G n 185 Ser Tr p Asp Asp Ser 265 Asp G u Phe Al a Met 345 Leu Thr 155 Asp G u Thr Lys Tr p 235 Sy Sy Ser G n G u 315 Thr Val SIle Ser Thr Ser Thr 205 bet SIle Al a Asn Hi s 285 I le G u Tyr Ar g Thr 365 Al a Hi s G u 190 Ar g Asn SIle Lys Sy 270 Tyr Thr Asp Su I e 350 Thr Asp G u 175 Ser Tr p Asp Hi s Al a 255 Tyr Phe Thr bet Phe 335 Leu Phe Pro Lys Pro G u Al a 375 <210> 413 <211> 816 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (101)..(793) <223> RXN00658 <400> 413 cattgacacc cacaggttta ccagcatcac ggaaagtttg gatggatttt tactccggcc acaacgtctg gctggaagct cagccacgtg ctttctggtc gtg cgc cac gac gag 115 Val Arg His Asp Gu 1 cac tac cca gct gcg gca aac ctc att gct ttc gat aag gga tgg tcc 163 His Tyr Pro Ala Ala Ala Asn Leu lie Ala Phe Asp Lys G y Trp Ser Page 490 IO seql i st cor r ect ed. t xt O 10 15 Sacc ctc atc gcc cct cag ctg gaa gat cca gag gcg gag gag ttc acc 211 Thr Leu lie Ala Pro On Leu u Asp Pro u Ala u u Phe Thr 30 d) 4 gcc gga ttc ctc acc gaa tac cag gac aat ctg atc act gcg ggc atg 259 Ala y Phe Leu Thr G u Tyr G n Asp Asn Leu Ile Thr Ala G y Iet 40 45 gag cac cag gcg ctc gcg agc ggc ttc ccg gtg ggg cgt cgc ttc aag 307 G u His G n Ala Leu Al a Ser G y Phe Pro Val G y Arg Arg Phe Lys C(1 55 60 00 tcc gat att gct tta cga cgc tgc gat gcg gtg acc acc cac atc ggc 355 SSer Asp Ile Ala Leu Arg Arg Cys Asp Ala Val Thr Thr Hi s lle y S 70 75 80 (N cac gaa cac tcc gcc gat ggt cac tgg agg atc tac gta ttc gct ggc 403 SHis G u His Ser Ala Asp G y His Trp Arg Ile Tyr Val Phe Ala G y 95 100 (N caa gcc acc cca caa gat tcc gag tct gca ctg aac aag tgg gcg cag 451 G n Ala Thr Pro G n Asp Ser u Ser Ala Leu Asn Lys Trp Ala G n 105 110 115 tgg atg gag gaa agc gaa gac tca cca ctc aac cgc ttc acc cca gaa 499 Trp Iet G u G u Ser G u Asp Ser Pro Leu Asn Arg Phe Thr Pro G u 120 125 130 gcc ggc gac cgc aac gca gtc ttc gat atc aag gct acc tac cag cag 547 Ala y Asp Arg Asn Al a Val Phe Asp Ile Lys Ala Thr Tyr n G n 135 140 145 cat tac cac tcc ttc gac ctg ttc gat gcg cca gag gtc ttc ttc cca 595 His Tyr His Ser Phe Asp Leu Phe Asp Ala Pro G u Val Phe Phe Pro 150 155 160 165 cga gtt gga cca tac aag ctg caa aac ctc gaa aac gtt tgg acc gca 643 Arg Val y Pro Tyr Lys Leu G n Asn Leu G u Asn Val Trp Thr Ala 170 175 180 ctg gat tcc caa gac atc ttt gag tcc cgt ggc atc agt cgc gat ggc 691 Leu Asp Ser n Asp Ile Phe u Ser Arg y Ile Ser Arg Asp y 185 190 195 gca att gtt gtc gtt cgc cca gac cag tac gtc gca gca gtc ctc cca 739 Ala lle Val Val Val Arg Pro Asp G n Tyr Val Ala Ala Val Leu Pro 200 205 210 ctc gaa gac acc gca gca ctg gct gag ttc ttc aat ggc aat ctg ctt 787 Leu G u Asp Thr Al a Al a Leu Al a G u Phe Phe Asn G y Asn Leu Leu 215 220 225 gag cca t aaaccct aa attctaggaa cga 816 G u Pro 230 <210> 414 <211> 231 <212> PRT <213> Cor ynebact eri um gl ut ari cum <400> 414 Val Arg His Asp u His Tyr Pro Ala Ala Ala Asn Leu lie Ala Phe 1 5 10 Page 491 seql i st cor r ect ed. t xt Ser Thr Leu lie Ala Pro n Leu G u Asp Lys G y Asp Pro u Al a SIle Gy Thr Tyr Asn Ar g Al a 145 G u Asn SIle Al a Asn 225 G u Al a Ar g Hi s Phe Tr p 115 Thr Tyr Phe Tr p Ar g 195 Val Asn Thr let Lys Gy Gy G n G u G n Pr o 165 Al a Gy Pr o Leu Al a G u Ser 70 Hi s G n Tr p Al a Hi s 150 Ar g Leu Al a Leu G u 230 Gy Hi s Asp G u Al a et Gy 135 Tyr Val Asp l Ie G u 215 Pr o Leu Al a Al a Ser Pr o 105 G u Ar g Ser Pr o G n 185 Val G u Al a Ar g Asp Asp G u Al a Asp 155 Lys SIle Ar g G n Sy Cys Hi s G u Ser 125 Phe Phe G n G u Asp 205 Asn Pr o Al a Ar g Al a Leu SIe Al a Leu 175 Ar g Tyr Thr Al a Al a Ala G u Phe Phe <210> 415 <211> 1008 <212> DNA <213> Corynebacter i um gl ut ari cum <220> <221> CDS <222> (101)..(985) <223> RXN00178 <400> 415 gcgaataatc cacggacggt cacagaagaa aacctcactg cgctgcttac cacagcgctc aacggcgacg atccagcaac tttgaattaa ggagaccaac atg act att tca gca 115 lMt Thr lie Ser Ala 1 caa cag caa gca gtg gaa gaa gac ctt gta gag cgc gta ctc gca tct 163 Sn n n Ala Val u u Asp Leu Val G u Arg Val Leu Ala Ser 15 ttt gat tcg tgt gaa aac cct cgc ctc aaa cta gtg atg aaa tcc ctg 211 Phe Asp Ser Cys G u Asn Pro Arg Leu Lys Leu Val IVet Lys Ser Leu 30 Page 492 seql i st cor r ect ed. t xt act gtg cat ctc cat gat Thr Val His Leu His Asp ttc atc cgc Phe II e Ar g gat gtt Asp Val cga ct c act Arg Leu Thr gaa gaa G u G u 45 gag G u gac Asp t cc Ser gaa G u caa G n gt g Val cgc Ar g 150 t ac Tyr aac Asn cca Pr o ccc Pr o cga Ar g 230 at c SIle gtt Val gat Asp tgg aac Trp Asn gat aag Asp Lys atg cag lMVt n gca aca Al a Thr aac gga Asn y 120 gag gga Gu Gy 135 att gaa Ile G u gcc gat Al a Asp ggc gaa G y G u cac gat His Asp 200 gtt cgt Val Arg 215 acc ttg Thr Leu ggc gat Sy Asp gag caa G u G n caa acc G n Thr gcc Al a caa G n at c I e ttt Phe gac Asp gt c Val tgg Tr p cgc Ar g 170 cac Hi s cca Pr o gcg Al a acc Thr gt g Val 250 gca Al a gca Al a att gat I e Asp 60 gaa ttc G u Phe 75 gct gtt Al a Val ggc ccc G y Pro att gcc I e Al a aaa gac Lys Asp 140 gaa t gc Su Cys 155 agt gct Ser Al a ttc tgg Phe Trp gt a gga Val G y cac ct a Hi s Leu 220 cat at c Hi s I I e 235 ttt ggc Phe G y gga acc G y Thr cgc aca Arg Thr ttc Phe gt g Val aat Asn ttc Phe ttt Phe 125 act Thr gat Asp gga Gy gga Gy caa G n 205 cac Hi s ttc Phe gt g Val gca Al a cgt Ar g 285 ct c Leu ttg Leu aac Asn ttt Phe 110 ggc gay gaa G u gaa G u cgc Ar g ct a Leu 190 at g Met ttc Phe gtt Val aag Lys act Thr 270 ttt Phe acc Thr ct c Leu gaa G u 95 gt c Val gca Al a gga Gy gat Asp gca Al a 175 act Thr ct c Leu at g Met gag G u gac Asp 255 cca Pr o aag Lys tct Ser 80 gca Al a gat Asp gt c Val aac Asn gga Sy 160 cac Hi s ccc Pr o caa G n gt g Val ggc G y 240 t ca Ser gat Asp gtt Val gac Asp t at Tyr gat Asp ggc G y ccc Pr o 145 ctt Leu ct g Leu gt g Val gca Al a act Thr 225 gat Asp ct g Leu ggt G y ggg cat G y Hi s acc ttg Thr Leu gaa gac Su Asp gcg cca Ala Pro 115 cag ccg G n Pro 130 att ccc Ile Pro tat gat Tyr Asp tat tca Tyr Ser cca t at Pro Tyr 195 gtt ggt Val G y 210 gcg cca Ala Pro ccg cag Pro G n att aag I e Lys cgc gat Arg Asp 275 at c SIle ggt G y gct Al a 100 ct g Leu gca Al a aat Asn gt g Val gat Asp 180 ccc Pr o cgt Ar g gag G u ct a Leu aaa Lys 260 gt g Val acc Thr gca Al a acc Thr gtc Val tgg Tr p gca Al a caa G n 165 gaa G u at c SIe t cc Ser aag Lys gag G u 245 ttc Phe ggt G y gat att gtg ctc gcc ccc ggc Asp lie Val Leu Ala Pro y 290 280 aat gt c Asn Val 295 taagtagaag cagcaaaaaa cca 1008 Page 493 seqI i st car r ect ed. t xt <210O> 416 <21 1> 295 <21 2> PRT fl<21 3> Cor ynebact er i urn gi ut ani cumn S<400> 416 I'bt Thr Ile Ser Ala(3n On OnAlaVal OuOu AspLeu Val Ou 1 5 10 Ar g Val Leu Ala Ser Phe Asp Ser Cys O31u Asn Pro Arg Leu Lys Leu 25 SVal I'bt Lys Ser Leu Thr Val Hi s Leu Hi s Asp Phe I Ie Ar g Asp Val 00 35 40 SArg Leu Thr O31u 31u O31u Tr p As n Tyr Ala Ilie Asp Phe Leu Thr Lys C150 55 SVa 1G31y Hi s Ilie Thr Asp Asp Lys Arg O31n O31u Phe Val Leu Leu Ser S 65 70 75 Asp Thr Leu G31y Ala Ser I'b t O31n Thr Ilie Ala Val Asn Asn Ou Ala 90 Tyr G u Asp Ala Thr G u Ala Thr Val Phe G y Pr o Phe Phe Val Asp 100 105 110 Asp AlaPr o Leu Val O31nAsn (1y Asp Asp Ile A aPhe(1y A a Val 115 120 125 O31y O31n Pr o Ala Tr p Val O31u O31y Thr Val Lys Asp Thr O31u O31y Asn 130 135 140 Pro Ilie Pr oAsn Aia Ar g Ile (1u Val Tr p 1u Cys Asp (1u Asp (1y 145 150 155 160 Leu Tyr Asp Val O31n Tyr Ala Asp O31u Ar g Ser Ala G31y Ar g Ala Hi s 165 170 175 Leu Tyr Ser Asp O31u Asn G31y O31u Tyr Hi s Phe Tr p G31y Leu Thr Pr o 180 185 190 Val Pr o Tyr Pr o Ilie Pr o Hi s Asp G31y Pr o Val G31y O31n I'b t Leu O31n 195 200 205 Ala Val G31y Ar g Ser Pr o Val Ar g Cys Ala Hi s Leu Hi s Phe I'b t Val 210 215 220 Thr Ala Pr o O31u Lys Ar g Thr Leu Val Thr Hi s Ilie Phe Val O31u G31y 225 230 235 240 Asp Pr o O31n Leu 31u Ilie O31y Asp Ser Val Phe O31y Val Lys Asp Ser 245 250 255 Leu Ilie Lys Lys Phe Val O31u O31n Pro Ala O31y Thr Ala Thr Pr o Asp 260 265 270 31y Ar gAsp Val O31yAsp (1n Thr Tr pAia Ar gThr Ar g Phe Asp Ilie 275 280 285 Val Leu Ala Pr o O31y Asn Val 290 295 <210> 417 <211> 135 <212> DNA Page 494 seql i st cor r ect ed. t xt <213> Cor ynebact eri um gl ut ai cum <220> <221> CDS <222> (101)..(712) <223> RXN01461 <400> 417 tcgactatga cgagacccgt gaaaacttcg cgcttggtta caagttcgac atcgtccttc gtggccgcaa cgccacccca tttgagtaaa gggttttgca aag aac ggc Lys Asn G y gaa gcc gag G u Al a G u cac at c ggt Hi s I Ie G y tcg gaa ggc Ser G u G y gac ccc at c Asp Pro Ile ggc atc cac G y I Ie Hi s gca gat ggc Al a Asp G y gag gca acg G u Al a Thr 120 cca cac ttc Pro His Phe 135 tac act cgc Tyr Thr Ar g 150 ttg gct gtg Leu Al a Val acc gat gat Thr Asp Asp gaa acc cca G u Thr Pro 200 <210> 418 ttc Phe ggc Sy acc Thr gt g Val gat Asp tct Ser cgc Ar g acc Thr gtt Val t ac Tyr cca Pr o 170 ttc Phe cgc Ar g at c SIle ctt Leu t cc Ser gcc Al a 75 gat Asp ggg G y act Thr ggt Sy ct g Leu 155 gct Al a cgc Ar g t ac Tyr act Thr gaa G u ttt Phe 60 at g Met ttg Leu ctt Leu ttg Leu gt g Val 140 cca Pr o gat Asp ttc Phe gag a u cct Pr o ggt Sy 45 act Thr ttt Phe gat Asp ggt G y gtt Val 125 ttc Phe gac Asp cga Ar g gac Asp cag G n t ca Ser gcg Al a gtt Val gaa G u cca Pr o cgc Ar g 110 ccg Pr o gcc Al a gcc Al a cgt Ar g at c SIle 190 tcg Ser 15 cag G n gag G u t cc Ser ct g Leu aac Asn 95 gcg Al a gga G y cgt Ar g gat Asp gac Asp 175 act Thr aat Asn acc Thr cat Hi s gca Al a tgg Tr p 80 cgc Ar g at g Met gca Al a ggc Sy ttg Leu 160 ct c Leu gt c Val atg att gat aca ggg IVet lie Asp Thr y 1 atc atc gat cag aac i e i e Asp G n Asn gtg ggc cct tac gtc Val y Pro Tyr Val ctc gtg gag cca ggt Leu Val G u Pro y act gat ggc aac ggc Thr Asp y Asn y cag gcc gat cca gag G n Ala Asp Pro G u aca gca cca gca acc Thr Ala Pro Ala Thr 100 gca aac gcg cag ggt Ala Asn Ala n G y 115 ttc gca gat gag gca Phe Ala Asp G u Ala 130 atg ctg gag cgt ctg IVet Leu G u Arg Leu 145 agc acc gac cca gtt Ser Thr Asp Pro Val 165 ctg gtg gct caa aag Leu Val Ala G n Lys 180 cag gct gaa gac aat G n Al a u Asp Asn 195 ttt ttt gga ctc Phe Phe G y Leu taaattgacc cgatctttat act Page 495 seql i st cor r ect ed. t xt <211> 204 <212> PRT <213> Corynebact eri um gl ut ari cum <400> 418 ebt Ile Asp Thr 1 I e Val Leu Thr G n Thr Al a Phe bet 145 Ser Leu G n Asp Pr o G u Gy Asp Pr o Al a 115 Asp G u Asp Al a G u 195 Gy 5 Asn Val Gy Gy G u Thr Gy Al a Leu Val 165 Lys Asn Al a SIle G u Pr o SIle Asp Al a Hi s 135 Thr Al a Asp Thr Lys Asn G y G u Phe Leu Al a Al a Asn Phe 105 Phe Lys Al a Val Gy 185 Phe Ar g SIle Leu Ser Al a 75 Asp Gy Thr Gy Leu 155 Al a Ar g Gy u Pr o Gy Thr Phe Asp Gy Val 125 Phe Asp Ar g Asp n Ser Al a Val G u Pr o Ar g 110 Pr o Al a Al a Ar g SIle 190 Ser G n G u Ser Leu Asn Al a Gy Ar g Asp Asp 175 Thr Asn Thr Hi s Al a Tr p Ar g let Al a Gy Leu 160 Leu Val <210> 419 <211> 1584 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1561) <223> RXN01653 <400> 419 ttcattcagg gtgaatgctc tccttgtttc agatgttcaa cgctccataa agtagaccgc aatctagaca aagatgtcta ttttaattaa ggagcagaac atg gcc acg gcc gag 115 lMt Ala Thr Ala G u 1 aac aca aca cag gag aat cgg aaa atc ctg ttc aac gca ttt gat atg 163 Asn Thr Thr n G u Asn Arg Lys Ile Leu Phe Asn Ala Phe Asp IVet 15 aac tgc gtt gcg cat cag tcc cca gga ctg tgg aca cac ccg aag gat 211 Asn Cys Val Ala His G n Ser Pro G y Leu Trp Thr His Pro Lys Asp Page 496 IO seql i st cor r ect ed. t xt 30 Saag gcg cga gac tac aac act ctt gat tac tgg gtg cac ctt gcc aag 259 Lys Ala Arg Asp Tyr Asn Thr Leu Asp Tyr Trp Val His Leu Ala Lys 40 45 d) 4 act ttg gag aag ggc ctt ttc gac ggc ctt ttc atc gca gat gtg ctt 307 Thr Leu G u Lys y Leu Phe Asp y Leu Phe lie Ala Asp Val Leu 60 gga act tac gat gtt tat ggt tct agt aat gaa gcg gcg ttg agc agt 355 Sy Thr Tyr Asp Val Tyr y Ser Ser Asn G u Ala Ala Leu Ser Ser CK1 70 75 80 O 00 ggt gcg cag gtg cct gtc aat gat ccg atc ctt ctt gtt tct gcg atg 403 0 G y Al a n Val Pro Val Asn Asp Pro Ile Leu Leu Val Ser Ala IVet 95 100 (N gcc tat gcc aca aag aac ctc ggg ttt ggc att act gca ggt act gcc 451 0 Al a Tyr Al a Thr Lys Asn Leu G y Phe G y I e Thr Al a G y Thr Al a 105 110 115 (N tat gag cac ccg tat cct ttt gcg cgg cgt ctg gcc aca ctt gat cac 499 Tyr G u His Pro Tyr Pro Phe Ala Arg Arg Leu Ala Thr Leu Asp His 120 125 130 ctg act aat ggg cgt gtg ggg tgg aat gtg gtt act ggc tat ctt ccc 547 Leu Thr Asn y Arg Val y Trp Asn Val Val Thr y Tyr Leu Pro 135 140 145 tct gct gct caa aac atg ggt gac acc gat cag ctg cca cat gat gag 595 Ser Ala Ala G n Asn IVet G y Asp Thr Asp G n Leu Pro His Asp G u 150 155 160 165 cgc tat gac aaa gca gat gaa tac ctg gaa gtg atc tac aag ctt ctc 643 Arg Tyr Asp Lys Ala Asp G u Tyr Leu G u Val lie Tyr Lys Leu Leu 170 175 180 gag ggc tcc tgg gaa gac gat gct gtt caa aac aat acg gag acg agt 691 G u y Ser Trp G u Asp Asp Ala Val G n Asn Asn Thr G u Thr Ser 185 190 195 gtc ttt acg gac tcc tcc aaa gtg cac gcc att aat cat cat ggc aag 739 Val Phe Thr Asp Ser Ser Lys Val His Ala IIe Asn His His G y Lys 200 205 210 tac ttt gat gtg ccg ggc att gcc atc act gag ccg agt gtg cag cgt 787 Tyr Phe Asp Val Pro G y Ile Ala lle Thr G u Pro Ser Val G n Arg 215 220 225 acg ccg gtg atc tac cag gcg ggt gca tcg ccg cgc gga ttg aaa ttc 835 Thr Pro Val IIe Tyr G n Ala G y Ala Ser Pro Arg G y Leu Lys Phe 230 235 240 245 gct ggt gag aat gca gaa gca gtg ttt atc aat tcc agc acc gtg gag 883 Ala Gy G u Asn Ala G u Ala Val Phe lie Asn Ser Ser Thr Val G u 250 255 260 gca atc acc aag act gtc gca aaa att cgc gct gct gcg gtc gct gcg 931 Ala lie Thr Lys Thr Val Ala Lys Ile Arg Ala Ala Ala Val Ala Ala 265 270 275 gga cgt gat cca cat gcg gtg aag atc ttt gcg atg caa acc atc atc 979 G y Arg Asp Pro His Ala Val Lys Ile Phe Ala IVet G n Thr lie lle 280 285 290 act ggt gaa aca gaa gca gat gcg cag gca aag ctg gag gaa tac agt 1027 Thr G y G u Thr G u Al a Asp Al a G n Al a Lys Leu G u G u Tyr Ser Page 497 IND seql i st cor r ect ed. t xt 295 300 305 Scgc tat atc gat cct gtc ggt ggt ctg acc ttg atg tct gga tgg acc 1075 Arg Tyr lie Asp Pro Val Gy y Leu Thr Leu IVet Ser y Trp Thr 310 315 320 325 d) 4 ggc gcg gat ctg tcg cag tat gac ctg gat gaa ccg atc acc aat att 1123 Qy Ala Asp Leu Ser G n Tyr Asp Leu Asp G u Pro lie Thr Asn Ile c- 330 335 340 gag tca aac gct att cag tcc act gca gcc acc att agc aac ggc acc 1171 Su Ser Asn Ala lie G n Ser Thr Ala Ala Thr lie Ser Asn y Thr C1 345 350 355 00 ggt gaa ggt gcg tgg acg gta cgc aaa ctg ggt gag gca acc ggc atc 1219 Gy Gu Gy Ala Trp Thr Val Arg Lys Leu y u Ala Thr y Ile 360 365 370 (N ggc ggc ttc gga cca gtg ctt gtg gga tct ggc gct aac gtt gcc gcg 1267 G y y Phe y Pro Val Leu Val y Ser y Ala Asn Val Ala Ala 375 380 385 (N gaa ctt gca cgc atc cag gat ctc agc gat gtt gat ggt ttc aac ctt 1315 G u Leu Ala Arg lie G n Asp Leu Ser Asp Val Asp y Phe Asn Leu 390 395 400 405 gct tat gcc atc acc cca gga act ttt gaa gat gtc gtg gac ttt gtg 1363 Ala Tyr Ala lie Thr Pro y Thr Phe G u Asp Val Val Asp Phe Val 410 415 420 gtg cct gag ctg caa aaa ctt agc cgc tac aag acg gaa tac gcg ccg 1411 Val Pro G u Leu G n Lys Leu Ser Arg Tyr Lys Thr G u Tyr Ala Pro 425 430 435 ggt tcc ttg cgc aac aaa ttg ctc ggt aaa ggt gat cgc ctg gac gat 1459 Sy Ser Leu Arg Asn Lys Leu Leu y Lys y Asp Arg Leu Asp Asp 440 445 450 acc cac cgc ggc gca agc tac cgc cta ggc gct cgg aac tcc acc gcc 1507 Thr His Arg G y Ala Ser Tyr Arg Leu G y Ala Arg Asn Ser Thr Ala 455 460 465 act att gat ctc agt tcc ata tcc gcc caa cta gtt tcc cag gga gcc 1555 Thr lie Asp Leu Ser Ser lie Ser Ala G n Leu Val Ser G n Gy Ala 470 475 480 485 cac tca tgatctcacc gcaaacaatc atc 1584 Hi s Ser <210> 420 <211> 487 <212> PRT <213> Cor ynebact eri um gl ut arri cum <400> 420 IVet Ala Thr Ala u Asn Thr Thr n u Asn Arg Lys Ile Leu Phe 1 5 10 Asn Ala Phe Asp Iet Asn Cys Val Ala His G n Ser Pro G y Leu Trp 25 Thr His Pro Lys Asp Lys Ala Arg Asp Tyr Asn Thr Leu Asp Tyr Trp 40 Val His Leu Ala Lys Thr Leu G u Lys G y Leu Phe Asp G y Leu Phe 55 Page 498 seql i st cor r ect ed. t xt I e Ala Asp Val Leu G y Thr Tyr 70 Asp Val Tyr G y Ser Ser Asn G u Al a Leu Thr Al a Thr 145 Leu SIle Asn Asn Pr o 225 Ar g Ser Al a vbt Leu 305 vbt Pr o SIle G u Al a 385 Asp Leu Ser Gy 115 Leu Tyr Hi s Lys G u 195 Hi s Val Leu Thr Val 275 Thr G u Gy Thr Asn 355 Thr Val Phe Ser Mbet Al a Hi s Pr o G u 165 Leu Ser Lys Ar g Phe 245 G u Al a SIle Ser Thr 325 I le Thr SIle Al a Leu 405 Gy Al a Ty r Leu Ser 150 Ar g G u Val Ty r Thr 230 Al a Al a Gy Thr Ar g 310 Gy G u Gy Gy G u 390 Al a Al a Tyr G u Thr 135 Al a Tyr Gy Phe Phe 215 Pr o Gy l Ie Ar g Gy 295 Tyr Al a Ser G u Gy 375 Leu Tyr G n Al a Hi s 120 Asn Al a Asp Ser Thr 200 Asp Val G u Thr Asp 280 Su I e Asp Asn Gy 360 Phe Al a Al a Val Thr 105 Pr o Gy G n Lys Tr p 185 Asp Val SIle Asn Lys 265 Pr o Thr Asp Leu Al a 345 Al a Gy Ar g l Ie Pr o 90 Lys Tyr Ar g Asn Al a 170 G u Ser Pr o Tyr Al a 250 Thr Hi s G u Pr o Ser 330 l le Tr p Pr o SIle Thr 410 Val Asn Pr o Val Met 155 Asp Asp Ser Gy G n 235 G u Val Al a Al a Val 315 G n G n Thr Val G n 395 Pr o Asn Leu Phe Sy 140 Sy G u Asp Lys SIle 220 Al a Al a Al a Val Asp 300 Sy Tyr Ser Val Leu 380 Asp Sy Asp Sy Al a 125 Tr p Asp Tyr Al a Val 205 Al a Sy Val Lys Lys 285 Al a Sy Asp Thr Ar g 365 Val Leu Thr Pr o Phe 110 Ar g Asn Thr Leu Val 190 Hi s l Ie Al a Phe SIle 270 I le G n Leu Leu Al a 350 Lys Sy Ser Phe I e Sy Ar g Val Asp G u 175 G n Al a Thr Ser SIe 255 Ar g Phe Al a Thr Asp 335 Al a Leu Ser Asp G u 415 Val Val Asp Phe Val Val Pro G u Leu G n Lys Leu Ser Arg Tyr Lys Page 499 seql i st cor r ect ed. t xt 420 425 430 Thr G u Tyr Ala Pro G y Ser Leu Arg Asn Lys Leu Leu y Lys y 435 440 445 Asp Arg Leu Asp Asp Thr His Arg G y Al a Ser Tyr Arg Leu G y Al a 450 455 460 Arg Asn Ser Thr Ala Thr Ile Asp Leu Ser Ser Ile Ser Ala G n Leu 465 470 475 480 Val Ser G n y Ala His Ser 485 <210> 421 <211> 702 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(679) <223> RXN02053 <400> 421 aaccagccag aaactatctc caaaagctaa taaaaccctt ctaccatgac tctgtttcca acacataaaa aggataaaaa gcactgacaa ataaggcgac gt c Val gct Al a gca Al a gat Asp ttc Phe gag G u gt c Val acg Thr cag G n gcg Al a acc Thr agt Ser gca Al a acc Thr gag G u gga Gy agg Ar g 120 gag G u acc Thr gag G u ttc Phe aag Lys cct Pr o ttg Leu at c SIle gcc Al a ttg Leu aac Asn ct g Leu aat Asn cag G n gt a Val 75 gaa G u aac Asn agc Ser agt Ser cgc Ar g at c SIle gcg Al a aag Lys act Thr gag G u ggt G y ct c Leu acc Thr 140 cgc Ar g gac Asp cag G n 45 ctt Leu ttc Phe at t SIle cgc Ar g gcg Al a 125 agc Ser gcc Al a aaa Lys caa G n t ac Tyr at t SIle ctt Leu agc Ser 110 gcg Al a ccg Pr o acc Thr 15 at c SIle cgt Ar g gag G u gcg Al a ggt G y 95 at t SIle gct Al a act Thr cgc Ar g gtt Val gaa G u gcc Al a gtt Val 80 acg Thr cag G n ttt Phe act Thr at g Met 1 aaa Lys gac Asp at t SIle t cc Ser gcc Al a gaa G u cag G n ct a Leu ggt 15y 145 t ac Tyr ctt Leu gtt Val cat Hi s cgc Ar g agg Ar g gca Al a at t SIle 130 tgg Tr p gat Asp ct g Leu at t SIle aaa Lys gaa G u gaa G u 100 gaa G u gct Al a gag G u gaa G u gag G u act Thr caa G n aac Asn cgt Ar g gca Al a gcc Al a gaa G u tca ctt tca gtc Ser Leu Ser Val Page 500 seql i st cor r ect ed. t xt aaa gtg aag gaa gca atc ggt ctc ggc ggg cgt gag gat cgt gca atc Lys Val Lys Gu Al a lie y Leu G y y Arg Ou Asp Arg Ala lie 150 155 160 165 gcc ctt gtt att gct acc gga ttc cct aat gaa cag ccg gag cac cct Ala Leu Val I e Al a Thr G y Phe Pro Asn G u G n Pro G u His Pro 170 175 180 ggt cgt ttg cag aat agg cgc atc gac aac agc tac taactctgcc Sy Arg Leu G n Asn Arg Arg Ile Asp Asn Ser Tyr 185 190 agctcgcccg gac <210> 422 <211> 193 <212> PRT <213> Corynebacter i um gl ut am cum <400> 422 Met Ser Leu Ser Val Val Ou Ala lle 1 Lys Asp SIle Ser Al a G u G n Leu Sy 145 G u Thr Al a Val G n Val Al a Ar g 115 Leu Asp Ar g 5 G u G u Thr G n Asn Ar g Al a Al a G u SIle 165 Al a Al a Asp Phe 70 G u Val Thr G n Lys 150 Al a Thr Ser 40 Al a Thr G u Gy Ar g 120 G u Lys Val Pr o Al a G n Al a Asp Phe 105 Asp Sy Su I e G n 185 Thr 10 G u Phe Lys Pr o Leu 90 I e Al a Leu Al a Al a 170 Asn Leu Asn G n Val 75 G u Asn Ser Ser l Ie 155 Thr Ar g SIle Al a Lys Thr G u Sy Leu Thr 140 Sy Sy Ar g Asp G n Leu Phe SIle Ar g Al a 125 Ser Leu Phe Al a Lys G n Tyr SIle Leu Ser 110 Al a Pr o G y Pr o Thr I e Ar g G u Al a Sy I e Al a Thr Sy Asn 175 Ar g Val G u Al a Val Thr G n Phe Thr Ar g 160 G u Sn Pro u His Pro y Arg Leu Asn Arg Arg lie Asp Asn Ser 190 <210> 423 <211> 1191 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS Page 501 seqi i st car r ect ed. t xt <222> (1168) <223> RXN00177 <400> 423 ct at tccaca gcagt ggacg ct gaacaact t caaacacag act tcacct c aact cagt tg t cggagcat a ggagct aaaa at t aagcagc t at cggat ct at g t ct t ta cag tt c l'bt Ser Leu 0 n Phe g at As p gcg A a at g vb t gca A a ccc Pr o at t I Ie aaa Ly s act Thr gcg A a at c I Ie 1 50 gct A a ccg Pr o gca A a g at As p tt t Ph e g aa (31u aat As n gt t ValI at t I Ie g aa (31u gt t ValI at t I Ie gca A a 120 aaa Ly s g at As p ggt G31y gcg A a t ct Ser 200 cgc Ar g t ct Ser ct c Le u gcc A a ggt G31y gt c ValI gca A a gt g ValI at g vb t t ct Ser act Thr gcg A a aat As n 1 70 ccc Pr o ggc G31y g aa (31u ggc G31y ggt G31y gct A a g at As p aag Ly s gaa (31u 75 t gt Cy s acc Thr gaa (31u ggt G31y t t a Le u 155 ggt G31y at c I Ie ct t Le u gcg A a t ct Ser c aa O31n g aa (31u tt c Ph e gt g ValI g aa (31u gt g ValI ac c Thr gca A a gt t ValI 140 ac c Thr tt g Le u aat As n ccc Pr o ct c Le u 220 ggt G31y cga Ar g at t I Ie gag (31u 45 t gg Tr p gca A a ggc G31y gca A a ac a Thr 125 g at As p at g vb t gct A a gcg A a aag Ly s 205 t ac Tyr ct c gt t ValI ag c Ser ct t Le u cat Hi s cgc Ar g ggt G31y Le u 110 aat As n aac As n t ct Ser c ac Hi s gct A a 1 90 at t I Ie ggt G31y c ac ct g Le u 15 cga Ar g ccc Pr a t ca Ser agt Ser gga G31y 95 ccg Pr a gt g ValI aaa Ly s tt g Le u t gc cys 1 75 at g vb t gt g ValI gcc A a tt c Ph e ct c Le u at g vb t aat As n gt t ValI 80 t cc Ser at c I Ie t gg Tr p gt g ValI ccg Pr a 1 60 at t I Ie gct A a gca A a t ac Tyr 1 ggt G31y g at As p gca A a gt c ValI 65 gcg A a ac a Thr at t I Ie gga G31y ct g Le u 145 gt a ValI g at As p gct A a g at As p ct g Le u 225 t ca Ser gcc A a cgg Ar g 50 gt g ValI aaa Ly s ac a Thr gcg A a tt g Le u 130 cca Pr a g aa (31u t ct Ser gag (31u gct A a 210 gct A a ggt G31y aaa Ly s 35 c aa O31n at g vb t g aa O31u ggt G31y gt a ValI 115 ac c Thr gt g ValI at g vb t tt g Le u gga G31y 1 c ag O31n gcg A a gag O31u 20 aac As n gt a ValI cat Hi s aac As n ct a Le u 1 00 ccc Pr a g aa O31u ac a Thr t cg Ser t gg Tr p 1 at t I Ie g ac As p gt g ValI gcg A a gt c ValI gca A a gt g ValI g ac As p gct A a act Thr gcc A a gt t ValI gt t ValI 1 gga G31y cga Ar g gt a ValI t ct Ser cac aag Hi s Lys at c t gc cac gt g tt g llieCys His Val Leu Leu Hi s Page 502 seql i st cor r ect ed. t xt 230 235 240 ggt gga act ttt aac ctt cca cac gcg caa acc cat gca G y G y Thr Phe Asn Leu Pro His Ala G n Thr His Al a 250 255 cct tat gtt ctt gcc ttc aac gcg cca tat gcg cca cag Pro Tyr Val Leu Ala Phe Asn Ala Pro Tyr Ala Pro G n 265 270 cgc gca gcg gca gct ttc ggt tct gcg aca gca ctt gaa Arg Ala Ala Ala Ala Phe y Ser Ala Thr Ala Leu G u 280 285 290 cag ctg cgt gcc caa gtg gga gca cca cag cga cta tcc G n Leu Arg Ala G n Val y Ala Pro G n Arg Leu Ser 295 300 305 ttc acc gca gca gga atc cca gag gca gtg gaa atc atc Phe Thr Ala Al a y Ile Pro u Ala Val u lie lle 310 315 320 gta ccg gcg aat aat cca cgg acg gtc aca gaa gaa aac Val Pro Ala Asn Asn Pro Arg Thr Val Thr G u G u Asn 330 335 ctg ctt acc aca gcg ctc aac ggc gac gat cca gca act Leu Leu Thr Thr Ala Leu Asn G y Asp Asp Pro Ala Thr 345 350 taaggagacc aacatgacta ttt <210> 424 <211> 356 <212> PRT <213> Corynebacter i um gl ut ai cum aca Thr gca Al a 275 gga Sy gat Asp ttg Leu ct c Leu ttg Leu 355 gt a Val 260 gaa G u ttg Leu t ac Tyr gag G u act Thr 340 aat Asn 245 ct g Leu caa G n caa G n gga Sy aaa Lys 325 gcg Al a 883 931 979 1027 1075 1123 1168 1191 <400> 424 Met Ser Leu G n Phe Asp His G u Thr Leu G y G n 1 Sy Asp Al a Val Al a Thr SIle Sy Leu 145 Sy Lys G n bet G u Sy Val 115 Thr Val 5 Al a Val Al a Val Asp Al a Thr Al a Val Al a bet Al a Pr o 70 I le Lys Thr Al a SI e 150 G n Val Asp I le Asp Al a Tyr Ar g 135 Tyr Asn Val 40 I le G u Val l Ie Al a 120 Lys Asp Leu Al a Asp Thr Val Al a 105 Sy Thr Ser 10 Al a Al a G u G y Asp Phe Val Lys Val Al a G u G u Val Cys Val 90 Met Thr Thr Ser G u Al a Thr G y Val 140 Ala Leu Thr 155 Page 503 Ar g SIle G u Tr p Al a Sy Al a Thr 125 Asp M\et Leu Ar g Pr o Ser Ser Sy Pr o Val Lys Leu seql i st cor r ect ed. t xt Val G u MVt Ser Val Al a Ser G y Leu Asn G y Leu Ala Hi s 165 170 Cys I I e 175 Asp Al a Asp Leu 225 I le Hi s Pr o Leu Leu 305 I le G u Al a Leu Gy 195 G n Al a Hi s Thr Al a 275 Gy Asp Leu Leu Leu 355 Gy Ar g Val Ser Leu 245 Leu G n G n Gy Lys 325 Al a Pr o Al a Asp Phe 230 Gy Pr o Ar g G n Phe 310 Val Leu Lys Leu Gy 215 Al a Gy Tyr Al a Leu 295 Thr Pr o Leu Al a Ser 200 Ar g Ser Thr Val Al a 280 Ar g Al a Al a Thr Asp 185 Al a Asp Al a Phe Leu 265 Al a Al a Al a Asn Thr 345 Pr o Gy G u Gy Asn 250 Al a Al a G n Gy Asn 330 Al a SIle Leu Al a Ser 235 Leu Phe Phe Val SIle 315 Pr o Leu Al a Lys 205 Tyr Leu Hi s Al a Ser 285 Al a G u Thr Gy Al a 190 I le Gy Hi s Al a Pr o 270 Al a Pr o Al a Val Asp 350 Met Val Al a Hi s G n 255 Tyr Thr G n Val Thr 335 Asp Al a Al a Tyr Lys 240 Thr Al a Al a Ar g G u 320 G u Pr o <210> 425 <211> 960 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(937) <223> RXC00963 <400> 425 ct gg acct at c SIle acc Thr gaa G u ctctga cggcgtcgac ttgttctgct tctctgatac accaattttc gaggccctta cgcacg tacttttact ccggaaggaa tctagaactt atg cgt ctt gca aca 115 IVet Arg Leu Ala Thr 1 cgc acc aac ggc acc acc att gct gct cgt gtt gaa tct gaa aac 163 Arg Thr Asn y Thr Thr Ile Ala Ala Arg Val u Ser G u Asn 15 gct acc acc atc gag ggc ttt gcc aac gtc ggt gaa tta ctc cag 211 Ala Thr Thr Ile u y Phe Ala Asn Val Gy u Leu Leu n 30 tcc aac tgg cgc gag ctg gca gaa aac gct gct ggt gag gct gtg 259 Ser Asn Trp Arg G u Leu Ala G u Asn Ala Ala Gy G u Ala Val Page 504 seqi i st car r ect ed. t xt ac c Thr at t I Ie cgc Ar g ct c Le u gct A a cgc Ar g gt g ValI 1 50 aag Ly s tt c Ph e ct g Le u g ac As p t ac Tyr 230 gt t ValI gt a ValI g aa O31u t gc cys ct c Le u gga G31y g ac As p 120 gt c ValI aac As n cca Pr o cct Pr o ac c Thr 200 gt c ValI tt g Le u c ac Hi s gt t ValI aag Ly s ggc G31y g at As p tt c Ph e g aa O31u c ag O31n t ac Tyr t gg Tr p 1 70 at g vb t ct c Le u ag c Ser gct A a cgt Ar g 250 at t I Ie ct a Le u aac As n cca Pr o g at As p gag (31u g at As p 140 ac c Thr c ag O31n ac c Thr ggC G31y g aa O31u 220 g ac As p cca Pr o G31y gca A a gcc A a ct t Le u gt c ValI 110 gca A a gct A a g at As p aag Ly s g at As p 1 90 aag Ly s ct c Le u at t I Ie cgc Ar g ggC G31y 270 gt a ValI aac As n tt t Ph e 95 gt t ValI gt t ValI gag O31u tt c Ph e t ct Ser 1 75 t ct Ser gt a ValI at c I Ie gt c ValI t ac Tyr 255 gt t ValI c ac Hi s 80 gt t ValI cca Pr a at c I Ie t ac Tyr c ag O31n 1 60 tt g Le u tt t Ph e c ag O31n g aa O31u ac c Thr 240 at c I Ie cca Pr a at t I Ie aag Ly s gag O31u at t I Ie at c I Ie 145 t ac Tyr g aa O31u gag O31u t cc Ser t ac Tyr 225 ggt O31y ggt (31y gca A a aaa Ly s tt c Ph e t gg Tr p ggC O31y 130 gct A a gca A a aag Ly s tt c Ph e ac c Thr 210 at c I Ie ac c Thr g ac As p cct Pr a g aa O31u cct Pr a gct A a 115 aag Ly s ggC O31y gca A a t cc Ser ggC O31y 1 cct Pr a ac c Thr cca Pr a (31y aag Ly s at g vb t g ac As p 1 00 aac As n cgc Ar g t ac Tyr cct Pr a gct A a 1 ggC O31y ac c Thr c ac Hi s ggC O31y g aa O31u 260 aag Ly s ggC O31y gcg A a aag Ly s gca A a gca A a gca A a 1 ggC O31y gag O31u aat As n at c O31y 245 ac c Th r t tc at t gaa aac Ph e I I e 03 u As n aag acg gt g Lys Thr Val 275 ttt gaa Phe 03 u t aaat gacaa ct t tccacga t ct <210O> 426 <21 1> 279 <21 2> PRT <21 3> Cor ynebact er i urn gi Ut ani cumn Page 505 seqI i st car r ect ed. t xt <400> 426 vb t ValI G31y A a Pr o li1e Ly s O31u I Ie I Ie 145 Tyr O31u O31u Ser Tyr 225 G31y G31y (31u Ar g Leu Ala Ser Le u O31u Pr o O31u Pr o A a 115 Ly s G31y A a Ser G31y 1 95 Pr o Thr Pr a G31y Ly s 275 Thr As n O31n ValI Ly s G31y A a Ly s A a A a A a 1 65 G31y O31u As n I Ie G31y 245 Thr ValI I Ie Ar g Thr As n (1y A a Ser Ph e ValI As p I Ie Le u Ar g 135 vb t Thr G31y A a Le u 215 Pr a G31y Ly s O31u Thr Tr p As n ValI Pr a Pr a 1 05 Tr p Ly s As p O31n Tr p 1 85 Tyr Ph e As p A a O31u 265 I Ie Ar g Ly s G31y As p 90 Ph e O31u G n Tyr Tr p 1 70 vb t Le u Ser A a Ar g 250 li1e Thr O31u O31u O31u Le u Thr As p G31y A a Thr 1 Hi s Thr O31u Pr a G31y 235 As n A a Thr Ilie Ala Ala Ph e A a As p Tyr Thr ValI vb t 125 A a Ar g G31y Pr a O31u 205 Ly s ValI O31n Le u A a (31u A a A a Le u ValI 110 A a A a As p Ly s As p 1 Ly s Le u I Ie Ar g G31y 270 As n As n ValI As n Ph e ValI ValI G u Ph e Ser 1 Ser ValI I Ie ValI Ty r 255 Ph e <210O> 427 <21 1> 1101 <212> DNA <213> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1078) <223> RXN00299 <400> 427 t gccat cggt t tggct at tg at tggaacaa gaaaggt gcc cagt ct gt tg caaagaagga Page 506 seql i st cor r ect ed. t xt atccatttcc gtctaatcgc taat t gcgag gagtctttgc t ca Ser agc Ser ttc Phe t ca Ser act Thr t at Tyr cgc Ar g ttg Leu gac Asp ggg Gy 150 ttg Leu cct Pr o gt g Val cct Pr o caa G n 230 at g M\et at t SIle aaa Lys cgc Ar g gct Al a cgt Ar g at c SIle gac Asp gct Al a 120 gt c Val aac Asn t ca Ser t cc Ser acc Thr 200 gt c Val cac Hi s ttg Leu ttt Phe t ca Ser tgg Tr p gca Al a ggt Sy gag G u ggc Gy cga Ar g ct g Leu at t SIle ct c Leu 170 gca Al a cgg Ar g gcg Al a gat Asp ggc G y 250 gcc Al a gt g Val tac Tyr gt g Val gcc Al a 75 cag G n ct g Leu cgc Ar g aac Asn cca Pr o 155 ttt Phe t cc Ser gat Asp gcc Al a tt c Phe 235 cga Ar g acc Thr gca Al a gca Al a ctt Leu ggc G y ttc Phe ggc G y gac Asp tct Ser 140 ggc G y ggt G y cac Hi s aac Asn gt c Val 220 t ac Tyr caa G n ttt Phe gcg Al a cat Hi s tct Ser gt c Val acc Thr gcc Al a 110 cag G n ct c Leu ggc Gy caa G n gca Al a 190 cag G n gt c Val gt a Val act Thr gag G u 15 caa G n cac Hi s cac Hi s at g t t ttg Leu 95 cct Pr o t cc Ser acc Thr acc Thr ttg Leu 175 cca Pr o cct Pr o acc Thr gcg Al a gat Asp 255 ggc G y aaa Lys aac Asn at c SIle ct g Leu 80 gcg Al a ggc G y gcc Al a ggc G y aac Asn 160 gca Al a act Thr t ca Ser gca Al a cgt Ar g 240 gag G u at g Met 1 gaa G u gct Al a at g Met ggt G y ccc Pr o gag G u acg Thr gag G u cgt Ar g 145 gt a Val gca Al a cac Hi s gag G u tct Ser 225 gca Al a caa G n tct atc cca ctt Ser Ile agg cct Arg Pro gaa ggt Su G y gag agc G u Ser gcg aac Al a Asn aac cac Asn Hi s ttg tac Leu Tyr gac at g Asp IVet 115 aac ttc Asn Phe 130 t cc cgt Ser Arg ccg ctg Pro Leu cag ttg G n Leu ctt gag Leu G u 195 cag cat G n Hi s 210 gat t cc Asp Ser cgc gtg Arg Val ctt gat Leu Asp Pr o ggt G y tta Leu at t SIle acc Thr t cc Ser cca Pr o 100 aat Asn ccg Pr o ct c Leu t ac Tyr ggt G y 180 cac Hi s cct Pr o act Thr aag Lys gaa G u 260 Leu gac Asp ggc G y tct Ser aag Lys cca Pr o gac Asp acc Thr t cc Ser cca Pr o at c SIe 165 at g Met gcg Al a gag G u gaa G u aac Asn 245 ct c Leu Page 507 at g Met gct Al a aag Lys act Thr 310 aat Asn gat tca cca gct gct cgc caa Asp Ser Pro Ala Al a Arg n 265 ata ggc act gga tcc gaa gtt l e G y Thr G y Ser G u Val 280 285 acg gca cag gct gat gaa ct g Thr Ala G n Ala Asp G u Leu 295 300 gaa gca acc acg cgc aat atg G u Ala Thr Thr Arg Asn IVet 315 tagtaccgat gggccggtag aca eql i st cor r ect ed. t xt att gtc gac atg ctt cac tac lie Val Asp IVet Leu His Tyr 270 275 aaa gaa tac cta gac ggt ttt Lys G u Tyr Leu Asp y Phe 290 atg atc tcc ctg caa tcc ccc IVet iIe Ser Leu G n Ser Pro 305 gaa att ctt gcg gat gcg tgg Su IIle Leu Ala Asp Ala Trp 320 931 979 1027 1075 1101 <210> 428 <211> 326 <212> PRT <213> Corynebacteri um gl ut am cum <400> 428 IVet Ser Ile 1 G u Al a bet Sy Pr o G u Thr G u Ar g 145 Val Al a Hi s Pr o Sy Ser Asn Hi s Tyr Met 115 Phe Ar g Leu Leu G u 195 Pro Leu Ser 5 G y Asp Ser Leu G y Phe Ile Ser Ser Thr Lys Thr 70 Ser Pro Tyr Pro Asp Ar g 100 Asn Thr Leu Pro Ser Asp Leu Pro G y 150 Tyr Ile Leu 165 G y Iet Pro 180 Hi s Al a Val Leu Phe Lys Al a I le Val SIle Ar g Val 135 Val Sy Tyr G n Asp Ar g 25 I le Pr o Leu Al a Leu 105 Leu G u Al a Ser Phe 185 Tyr Phe 10 Ser Tr p Al a Sy G u 90 Sy Ar g Leu l Ie Leu 170 Al a Ar g Al a Val Tyr Val Al a 75 G n Leu Ar g Asn Pr o 155 Phe Ser Asp Thr Al a Al a Leu Sy Phe Sy Asp Ser 140 Sy Sy Hi s Asn SIle Leu G u I le Sy Sy Ar g Pr o 125 Tyr Lys Al a Phe Tyr 205 Phe Al a Hi s Ser Val Thr Al a 110 G n Leu Sy G n Al a 190 G n G u G n Hi s Hi s bet Leu Pr o Ser Thr Thr Leu 175 Pr o Pr o Sy Lys Asn SIe Leu Al a Sy Al a Sy Asn 160 Al a Thr Ser Su n His Pro u Pro Tyr Val Ile Ala Ala Val Asn Val Thr Ala Page 508 INO seql i st cor r ect ed. t xt 210 215 220 Ser Asp Ser Thr G u G n Ala His Asp Asp Phe Tyr Lys Val Ala Arg 225 230 235 240 d) Al a Arg Val Lys Asn ebt Al a Leu Arg G y Arg G n Val Thr Asp G u 245 250 255 G n Leu Asp G u Leu Iet Asp Ser Pro Ala Ala Arg G n Ile Val Asp 260 265 270 IVet Leu Hi s Tyr Thr Al a I e G y Thr G y Ser G u Val Lys G u Tyr Cr 275 280 285 0 00 Leu Asp y Phe Val Lys Thr Ala G n Ala Asp G u Leu IVet lie Ser 290 295 300 C( Leu G n Ser Pro Asn Thr G u Ala Thr Thr Arg Asn Iet G u Ile Leu 305 310 315 320 Ala Asp Ala Trp lie Asn C 325 <210> 429 <211> 784 <212> DNA <213> Corynebacter i um gl utam cum <220> <221> CDS <222> (18)..(761) <223> FRXA00299 <400> 429 gggtgccggc ggcggtc atg ctg ccc aac cac tcc cca tat gtc atc gct gag 53 IVet Leu Pro Asn His Ser Pro Tyr Val I e Al a Gu 1 5 cag ttc ggc acc ttg gcg gag ttg tac cca gac cgc atc gac ctc ggc 101 G n Phe y Thr Leu Ala G u Leu Tyr Pro Asp Arg lie Asp Leu y 20 atg ggc cgt gcc cct ggc acg gac atg aat acc ttg cgc gct tta cga 149 Iet G y Arg Ala Pro G y Thr Asp IVet Asn Thr Leu Arg Ala Leu Arg 35 cgc gac cct cag tcc gcc gag aac ttc ccg tcc gac gtt gtc gag ctg 197 Arg Asp Pro G n Ser Al a G u Asn Phe Pro Ser Asp Val Val G u Leu 50 55 aac tct tac ctc acc ggc cgt tcc cgt ctc cca ggg gtt aac gca att 245 Asn Ser Tyr Leu Thr G y Arg Ser Arg Leu Pro y Val Asn Ala lie 70 cca ggc aag ggc acc aac gta ccg ctg tac atc ttg ggt tca tcc ctc 293 Pro y Lys y Thr Asn Val Pro Leu Tyr lie Leu y Ser Ser Leu 85 ttt ggt gca caa ttg gca gca cag ttg ggt atg cct tat tcc ttc gca 341 Phe G y Al a n Leu Al a Al a G n Leu G y IVet Pro Tyr Ser Phe Al a 100 105 tcc cac ttc gca cca act cac ctt gag cac gcg gtg caa acc tac cgg 389 Ser His Phe Ala Pro Thr His Leu G u His Ala Val G n Thr Tyr Arg 110 115 120 gat aac tac cag cct tca gag cag cat cct gag cct tat gtc att gcg 437 Page 509 Asp Asn Tyr n Pro Ser G u 125 130 gcc gtc aat gtc acc gca tct Ala Val Asn Val Thr Ala Ser 145 ttc tac aag gta gcg cgt gca Phe Tyr Lys Val Ala Arg Ala 160 cga caa gtt act gat gag caa Arg n Val Thr Asp u n 175 gct cgc caa att gtc gac atg Ala Arg n Ile Val Asp IVet 190 195 tcc gaa gtt aaa gaa tac cta Ser G u Val Lys G u Tyr Leu 205 210 gat gaa ctg atg atc tcc ctg Asp G u Leu IVet lie Ser Leu 225 cgc aat atg gaa att ctt gcg Arg Asn Iet u Ile Leu Ala 240 gggccggtag aca <210> 430 <211> 248 <212> PRT <213> Cor ynebact er i um gl ut ar seql i st cor r ect ed. t xt G n His Pro u Pro Tyr Val lie 135 gat tcc act gaa caa gcc cac gat Asp Ser Thr G u G n Ala His Asp 150 155 cgc gtg aag aac atg gca ttg cgt Arg Val Lys Asn IVet Ala Leu Arg 165 170 ctt gat gaa ctc atg gat tca cca Leu Asp G u Leu IVet Asp Ser Pro 180 185 ctt cac tac acc gct ata ggc act Leu Hi s Tyr Thr Al a lie G y Thr 200 gac ggt ttt gta aag acg gca cag Asp y Phe Val Lys Thr Ala G n 215 caa tcc ccc aac act gaa gca acc G n Ser Pro Asn Thr G u Ala Thr 230 235 gat gcg tgg att aat tagtaccgat Asp Ala Trp lie Asn 245 <400> 430 IVet Leu Pro Asn His Ser Pro Tyr Val Ile Ala u n Phe 1 Leu Pr o Ser Thr Thr Leu Pr o Pr o Thr G u Thr G u Ar g Val Al a Hi s 115 G u Ser 5 Tyr bet Phe Ar g Leu Leu G u Hi s Ser Asp Thr Ser Pr o SIle et Al a G u 135 G u I e Ar g Val Val Sy Tyr 105 G n Tyr Al a 10 Asp Leu G y Ala Leu Arg Val G u Leu Asn Ala lle 75 Ser Ser Leu 90 Ser Phe Al a Thr Tyr Arg Val Ile Al a 140 Hi s Asp Asp Page 510 Met Ar g Asn Pr o Phe Ser Asp 125 Al a Phe G y Thr Arg Ala Pro G n Tyr Leu Lys G y Al a n Phe Al a Tyr G n Asn Val Lys Val 145 Al a Asp Val G u I e 225 I le Al a G n Met 195 Leu Leu Al a Val 165 Asp Hi s Gy Ser Al a 245 Asn Leu Thr Val 215 Asn l Ie seql i st cor r ect ed. t xt 155 IVet Ala Leu Arg Gy Arg On Val 170 175 et Asp Ser Pro Ala Ala Arg G n 185 190 Al a I e G y Thr G y Ser G u Val 200 205 Lys Thr Ala G n Ala Asp G u Leu 220 Thr G u Ala Thr Thr Arg Asn IVet 235 Asn <210> 431 <211> 825 <212> DNA <213> Cor ynebact eri um gl ut ari cum <220> <221> CDS <222> (101)..(802) <223> RXA00332 <400> 431 aatgaactct ggaaccgcca tgcagcaaaa cctctccaat gttacgccag ccctgcgaca ccaccatcta gggttagagt tggtaatctt tgactcccag gcg Al a cca Pr o agc Ser tgg Tr p gat Asp at g Met ctt Leu gat Asp gat Asp ggc Sy gaa G u cga Ar g cag G n tct Ser ct c Leu ct c Leu ctt Leu gaa G u gcc Al a gtt Val acg Thr acc Thr cat Hi s cac Hi s cgc Ar g ct a Leu at a l Ie cca Pr o t at Ty r 75 tct Ser ccg Pr o gga G y gcc Al a ggg G y caa G n ct c Leu tta Leu aca Thr ct a Leu cgt Ar g cct Pr o cgc Ar g gca Al a 45 gca Al a gat Asp at c SIle cat Hi s ttc Phe caa atc acc G n Ile Thr 15 agt gaa at g Ser G u Vbet gaa gct caa G u Al a n gtt cct caa Val Pro G n gat cca ttc Asp Pro Phe 80 gcg ctg ggc Al a Leu G y 95 gtg gcg aaa Val Al a Lys 110 gtt tta ggc Val Leu G y atg gcc Vet Al a 1 ctc gga Leu G y gtt cca Val Pr o gga ttc G y Phe gga at c G y lle tta at g Leu IVet act gca Thr Al a tcc gcg Ser Al a ttc aac aaa Phe Asn Lys ct c at g aca Leu IVet Thr acc gaa aat Thr G u Asn aga ggc atg Arg y IVet act gtt acc Thr Val Thr ctc ggt gcg Leu G y Al a gcg acc gtg Al a Thr Val 100 ctc acc ctt Leu Thr Leu 115 atc ggc tct ggc gac I e G y Ser G y Asp Page 511 seql i st cor 120 125 agg cct gaa gaa ttc gag att ttt ggc aaa Arg Pro u u Phe u Ile Phe y Lys 135 140 gct gat att cag tct ggg tgg gca att ttg Al a Asp Ile n Ser G y Trp Al a lie Leu 150 155 gat cct gcg atg cgg gcc gac ctt gaa ttt Asp Pro Ala IVet Arg Al a Asp Leu G u Phe 170 175 gaa gct cag atc ccc atg atc gct gta ggt Su Ala n Ile Pro bt I le Ala Val y 185 190 caa tgg atc gcc cga aac gcc gac gga tgg Sn Trp lie Al a Arg Asn Al a Asp G y Trp 200 205 gct gaa gct caa gtc gga cgc ctc gat ct c Ala G u Ala G n Val G y Arg Leu Asp Leu 215 220 ggc acc cgc cct tgt tgatttcctc catggggct Gy Thr Arg Pro Cys 230 <210> 432 <211> 234 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 432 Iet Ala Phe Asn Lys Ala Tyr Asp Ala Leu 1 5 10 Leu y Leu IVet Thr Pro Asn y Pro G u 25 Val Pro Thr G u Asn Ser Ile G u Leu Ala 40 Sy Phe Arg Gy IVet Trp Val Arg Asp Val Gy Ile Thr Val Thr Asp Lys G n Ala Thr 70 Leu IVet Leu y Ala IVet Ala Ser Val Thr 90 Thr Ala Ala Thr Val Leu Pro Leu Arg His 100 105 Ser Ala Leu Thr Leu Asp Arg Leu Ser His 115 120 IIe G y Ser G y Asp Arg Pro G u G u Phe 130 135 Leu Asp Asn Arg Arg Al a Asp lie G n Ser 145 150 r ect ed. txt 130 agc tta gac Ser Leu Asp 145 cgt gca gct Ar g Al a Al a 160 gcg cca acc Al a Pro Thr t ct gcc cga Ser Al a Ar g gca acc t ac Al a Thr Tyr 210 tgg gac aaa Trp Asp Lys 225 c aac aat Asn ttg Leu acg Thr caa G n 195 t ac Tyr gcc Al a cga Ar g tcg Ser cca Pr o 180 aca Thr cgc Ar g cgt Ar g Arg Ala Pro G n Ile Thr Leu l Ie Pr o Tyr Ser Pr o Sy G u Sy 155 Ar g Al a Al a Asp SIle Hi s Phe 125 Phe Al a Ser G u Val Asp Al a Val 110 Val G y lie G u Al a Pr o Pr o Leu Al a Leu Lys Leu Ala Ala Leu Ser Pro Asp Pro Ala IVet Arg Ala Asp Leu u Phe Ala Page 512 seql i st cor r ect ed. t xt 165 Pro Thr Thr Pro Pro u Ala G n Ile 180 185 Ala Arg G n Thr Val n Trp Ile Ala 195 200 170 Pro Met Ile Ala 175 Val G y Ser 190 G y Trp Al a Arg Asn Ala Asp 205 Thr Tyr Tyr Arg Pro Al a u Ala G n Val Gy Arg 210 215 220 Asp Lys Ala Arg y G y Thr Arg Pro Cys 225 230 Leu Asp Leu Trp <210> <211> <212> <213> <220> <221> <222> <223> 433 842 DNA Cor ynebact eri um gl ut am cum CDS (819) RXA01838 <400> 433 cag cac ctc tcc ggc G n Hi s Leu Ser G y 1 5 ggc cgt gtt gac G y Arg Val Asp atg atg ggc cgt IVet IVet y Arg ggc aac G y Asn acc gga ccc Thr G y Pro cca cta gcg Pro Leu Ala tac cca tgg ttt Tyr Pro Trp Phe ggc Gy aaa gac atc cac Lys Asp Ile His caa ggc atc Sn G y lie tgg cgc gaa Trp Arg G u att gaa aac tac lie G u Asn Tyr ctc ctg cgc cgc Leu Leu Arg Arg ct c Leu gac gta Asp Val gtc aac tgg cag Val Asn Trp n ggc Gy aaa ttc cgc aca Lys Phe Arg Thr ttg cag gga tac Leu G n G y Tyr acc Thr tct acc cca gca Ser Thr Pro Ala cca Pr o 70 tta gac ggc gtt Leu Asp G y Val gca Al a cca ttc gtc tgg Pro Phe Val Trp ggc tcc atc cgc Sy Ser lie Arg t cc Ser acc gaa atc gca Thr G u I e Al a caa gca gcc ttc G n Ala Ala Phe tat ggc Tyr G y gac ggc ttc Asp G y Phe caa at g gt c G n Mbt Val 115 cac aac aac atc His Asn Asn Ile ttc Phe 105 tgg aac aaa gag Trp Asn Lys u cac acc gcc Hi s Thr Al a 110 aac ctc tac cgc Asn Leu Tyr Arg cag cgt ttc gaa cac tac gga cac ggc G n Arg Phe G u His Tyr y His y 120 125 caa gca G n Al a 130 gac cag gcc atc Asp G n Ala li e gt g Val 135 gga ctc ggt ggc G y Leu G y G y caa G n 140 gtc ttc atc ggc Val Phe lie Gy gat Asp 145 tct gaa gaa gaa Ser G u G u G u gca Al a 150 aag aag acc ttc Lys Lys Thr Phe cgc Ar g 155 ccc tac ttc gac Pro Tyr Phe Asp gcc cct gtc tac gga cac gga cca tca Ala Pro Val Tyr Gy His Gy Pro Ser ctt gaa gat ttc tcc cgc ctg Leu G u Asp Phe Ser Arg Leu Page 513 ac c Thr g aa (31u g ac As p ct c Le u 225 cgc Ar g gcc Al a gag (31u seqI i st car r ect ed. t xt 165 170 cca cta acc gt c ggt acc gct gag caa gtt atc gaa Pr o Leu Thr Val G31y Th r Al a 31u O31n Val I Ie O31u 180 185 t tc gcc gac t gg gt a ggc gat t ac cag cgc cag ct c Phe Al a As p Tr p Val G31y As p Ty r O31n Ar g O31n Leu 195 200 205 cac gcc ggc ct g cca ct a gaa at g gt c ct t gat cag Hi s Al a (1y Leu Pr o Leu (1u I'b t Val Leu As p (1n 210 215 220 ggc cac gat gtc gt c cca gag gta cgc cgc cgc atg O31y Hi s Asp Val Val Pr o O31u Val Ar g Ar g Ar g I'b t 230 235 cca gac cac gtt ccc tcc aac cca cca acc cac cag Pr o Asp Hi s Val Pr o Ser Asn Pr o Pr o Thr Hi s O31n 245 250 aac cga aac agc cct t ac t tt cag at c aac cct ggt Asn Ar g Asn Ser Pr o Tyr Phe O31n Ilie Asn Pr o G31y 260 265 t agt tt t tct gaaactaagg aga 1 ac c Th r ct c Le u g aa O31u gag O31u ct g Le u 255 cca Pr o <210O> 434 <21 1> 273 <21 2> PRT <21 3> Cor ynebact er i urn gl ut anm cumn <400> 434 O3 n Hi s LE Thr 03 y Pr Pr o Leu Al As p Val V, Thr Ser T~ G31y Ser I I As p 03 y P~ O3 n Nib t V, 11 O3 n Al a A,, 130 As p Ser 03 145 .u Ser o Val a I Ie 1l As n h r Pr o e Ar g he Phe 1 00 Il As n '5 ~p 03 n u (1u G31y Ty r O31u Tr p Al a Ser Hi s Le u Al a O31u G31y Ar g ValI Asp Pr o As n O31n Pr o 70 Th r As n Ty r I Ie Al a 150 Tr p Ty r G31y 55 Le u O31u As n Ar g ValI 135 Ly s G31y Le u Ph e G31y Al a Ph e 1 Ar g Le u Th r vb t As p Ar g Th r Al a O31n As n O31u O31y Ar g 1 vb t I Ie Ar g Pr o Pr o Al a Ly s Hi s O31n 140 Pr o O31y Hi s Le u Le u Ph e Al a O31u Ty r 125 ValI Ty r O31y O31y Ar g O31y Tr p Ty r Th r Hi s I Ie As p Ala Pro Val Tyr Oy His Oy Pro Ser Leu Ou Asp Phe Ser Arg Leu Page 514 Thr G31u As p Le u 225 Ar g A a (31u Le u A a 1 95 A a Hi s As p Ar g 1 65 ValI Tr p Le u ValI ValI 245 Ser Thr G31y Le u 215 Pr o Ser Ty r seqI i st car r ect ed. t xt 1 70 Ala Ou On Val Ilie Ou 1 As p Ty r (31n Ar g O31n Leu 200 205 O31u I'b t Val Leu As p O31n 220 O31u Val Ar g Ar g Ar g [Vb t 235 As n Pr o Pr o Thr Hi s O31n 250 Phe O31n IIe As n Pro G31y 265 1 Th r Le u O31u O31u Le u 255 Pr o <210O> 435 <21 1> 1167 <21 2> DNA <21 3> Cor ynebact er i urn gi ut ani cumn <220> <221 OIDS <222> (1144) <223> RXA02643 <400> 435 tgagaacacc taaaaacctt gcgggaatac caccaacccc at at tgt tga t at at ctaca aact ctt t ta at aagt ct ga t caacaacgt gag gaaag ca tt c Ph e ccg Pr o at g vb t cgt Ar g ct c Le u t cc Ser gca A a ct c Le u t cc Ser g ac As p g aa O31u t cc Ser g at As p t cc Ser ac c Thr gca A a gt c ValI t ca Ser gag O31u cct Pr o ggt (31y t t c Ph e caa O31n ggg G31y gt t ValI cga Ar g 75 gt t ValI cgc Ar g ggC G31y ac a Thr gt g ValI t ct Ser ct c Le u cgc Ar g gcc A a g ac As p ct t Le u g at As p 45 gca A a aaa Ly s ct g Le u g aa (31u aac As n cga Ar g at c I Ie cct Pr a gt c ValI tt t Ph e at c I Ie 110 gcc A a 15 aac As n at c I Ie g ac As p ac c Thr gag (31u 95 ac c Thr at c I Ie at c G31y at c I Ie t ct Ser cgt Ar g tt g Le u at g vb t 1 g ac As p at t I Ie at c I Ie gt c ValI t cc Ser t at Tyr gga (31y aaa Ly s ct g Le u g at As p gga G31y at g vb t gt g ValI t cc Ser cgc Ar g gt c ValI ggC G31y gcc A a c ac Hi s gct A a gt g ValI at g vb t 1 00 t cc Se r Page 515 seql i st cor r ect ed. t xt att gag tct i e u Ser 120 ctg ttt agt Leu Phe Ser 135 agc cgt ggt Ser Arg G y 150 gaa aac cag Su Asn n gtt gca gt g Val Al a Val cgt ttc ccg Arg Phe Pro 200 cgc ccg tat Arg Pro Tyr 215 cct caa aag Pro G n Lys 230 gat gag gaa Asp G u u cgc aag at c Arg Lys Ile ttt gaa cgc Phe G u Ar g 280 acg gtc gca Thr Val Al a 295 cgc ttt acc Arg Phe Thr 310 ct g aag t cc Leu Lys Ser gac atc ttg Asp Ile Leu ttc cca ttg ttt Phe Pro Leu Phe cgc Ar g ggc G y ctt Leu 170 ggc G y at g Met gat Asp at t SIle cgt Ar g 250 gct Al a at c SIle aag Lys aaa Lys gag G u 330 gat Asp 140 acc Thr cca Pr o cca Pr o gcc Al a t ac Tyr 220 gt g Val ct a Leu cgc Ar g cac Hi s gcc Al a 300 gcc Al a t at Tyr ggt Gy 125 ttg Leu tgg Tr p cca Pr o gaa Gu at c l Ie 205 aag Lys cac Hi s gca Al a ggt Gy ggt Gy 285 aaa Lys agt Ser ggc G y ttt gat Phe Asp ttc Phe cat Hi s act Thr t ca Ser 190 at c SIle cgt Ar g t cc Ser ctt Leu tgg Tr p 270 t cc Ser acc Thr ggc G y act Thr gcg Al a ggt G y gag G u 175 gt c Val ggc G y gcc Al a cct Pr o aat Asn 255 gct Al a tta Leu at t SIle cag G n gag G u 335 ctt cag gac Leu G n Asp 130 aag att ctt Lys IIe Leu 145 gag acc cgc G u Thr Arg 160 aat ggc at t Asn G y II e gtg cgc gct Val Arg Ala ggt gct cct G y Al a Pr o 210 aac gaa cag Asn G u n 225 gga ct c at t G y Leu Ile 240 gat tgg ttg Asp Trp Leu cct gcg gat Pro Al a Asp t ac at c ggt Tyr Ile G y 290 tca gtg ct t Ser Val Leu 305 acc cct cat Thr Pro His 320 gtt att ccg Val Ile Pro t ac Tyr gag G u tcg Ser cac Hi s gct Al a 195 gag G u ttc Phe gcg Al a gaa G u gca Al a 275 t cc Ser gat Asp gag G u ct g Leu gag G u gcc Al a gcg Al a gct Al a 180 aag Lys cgt Ar g ggg Gy gca Al a ct c Leu 260 at g Met cct Pr o ctt Leu t ac Tyr gt g Val 340 499 547 595 643 691 739 787 835 883 931 979 1027 1075 1123 1167 aag cag gct Lys G n Al a taagaaggtc ttaggacatt ccc <210> 436 <211> 348 <212> PRT <213> Corynebacter i um gl ut ai cum <400> 436 Page 516 seqi i st car r ect ed. t xt l'bt Lys Asn Val Ser Phe (1y Leu Asp Thr Phe (1y Asp 1 5 10 As n A a I I e As p I Ie I Ie ValI Ser Tyr O31y O31n I Ie 145 Thr O31y Ar g A a O31u 225 Le u Tr p A a I Ie ValI 305 Pr o I Ie O31n O31u O31u Thr Thr Thr O31y 115 Tyr O31u Ser Hi s A a 1 95 O31u Ph e A a O31u A a 275 Ser As p O31u Le u As n Ly s Hi s I Ie Le u As n Ph e O31n As p Le u 1 65 Tr p Tyr Ph e O31n Thr 245 O31n O31n O31u As p Le u 325 Ly s ValI A a O31u A a Ser Le u O31u Ph e 135 Ar g As n A a Ph e Pr o 215 O31n O31u Ly s O31u ValI 295 Ph e Ly s I Ie Pr o Ly s Tyr Thr As p As n 1 Ph e O31u O31n vb t O31y 1 Le u ValI Pr o A a O31y 265 O31u Ly s Le u I Ie Thr 345 O31n O31y ValI Ar g ValI Ar g Le u Le u ValI 1 Tyr Ser Le u Le u O31y 235 O31u O31u As p I Ie Tyr 315 Le u O31n Thr ValI Ser Le u Ar g A a Ph e As p 140 Thr Pr a Pr a A a Tyr 220 ValI Le u Ar g Hi s A a 300 A a Tyr A a Le u As p A a Ly s Le u O31u O31y 125 Le u Tr p Pr a Ou le 205 Ly s Hi s A a O31y O31y 285 Ly s Ser O31y As n I Ie As p Thr O31u Thr As p A a O31y O31u 1 ValI O31y A a Pr a As n 255 A a Le u I Ie O31n O31u 335 <210O> 437 Page 517 seql i st cor r ect ed. t xt <211> 726 <212> DNA <213> Cor ynebact eri um gl ut am cum <220> <221> CDS <222> (101)..(703) <223> RXA01933 <400> 437 ctagaagcct taggcaagaa atttggttga gttttcgatc ttccacctag gcttggatgc aggttagaaa ggagccttcg act Thr gcg Al a gac Asp ggc Sy ggt Gy ggc Sy aag Lys ttg Leu gat Asp aag Lys 150 gt g Val gaa G u ct g Leu ttt Phe ct g Leu gt g Val gag G u t at Tyr aaa Lys aag Lys 120 gt g Val ttc Phe ttt Phe ct c Leu gat Asp cgg Ar g gag Gu gag G u ttg Leu cgt Ar g cac Hi s ttg Leu ttg Leu gt g Val gat Asp 170 aca ctt Leu gtt Val gtt Val gt g Val gat Asp 75 gat Asp cgc Ar g aag Lys tcg Ser gca Al a 155 gat Asp at t t at Tyr gcg Al a t at Tyr aat Asn at c SIle cag G n at c SIle gag G u tgc Cys 140 ctt Leu cgc Ar g cac gtt Val gcg Al a tcg Ser tgg Tr p gag G u at g Met at t SIle 110 aat Asn at t SIle gcc Al a cgt Ar g act at c SIle ggg Gy cgt Ar g cag G n at c I e 80 gat Asp tcg Ser gag G u gcg Al a ggt Gy 160 at t SIle tta Leu t ctacgacgc gtcatctcaa atg tct aag act cgt 11! IVet Ser Lys Thr Arg 1 aag gag cat ggt gcg 16 Lys G u Hi s G y Al a gag ccg tcc aag aac 21 G u Pro Ser Lys Asn ctg gat ctg gat gcc Leu Asp Leu Asp Al a atc aaa cta ttg gtg Ile Lys Leu Leu Val gcg gct gac tac agg Ala Ala Asp Tyr Arg tat gtg ttg tcg ttg Tyr Val Leu Ser Leu 100 aat att ccg gag ggg Asn lie Pro Gu Gy 115 tgg ctt gat cag ctt 49! Trp Leu Asp G n Leu 130 gcg aag ccg gag ccg 54 Ala Lys Pro G u Pro 145 gaa aaa gct gag gat 59! G u Lys Al a G u Asp 165 gag gca gcg cgc gaa 64; G u Ala Ala Arg G u 180 gat tcc tta aaa gaa 69 Asp Ser Leu Lys G u 195 Thr Ile His Phe Thr agc att cag gaa Ser Ile G n u 200 tgacacctca accactgatt ttg Page 518 seql i st cor r ect ed. t xt <210> 438 <211> 201 <212> PRT <213> Corynebacteri um gl ut am cum <400> 438 IVet Ser Lys Thr Arg 1 5 Lys G u His G y Al a G u Pro Ser Lys Asn Leu Asp Leu Asp Ala Ile Lys Leu Leu Val Ala Ala Asp Tyr Arg Tyr Val Leu Ser Leu 100 Asn lie Pro Gu y 115 Trp Leu Asp G n Leu 130 Ala Lys Pro G u Pro 145 G u Lys Al a G u Asp 165 G u Ala Ala Arg G u 180 Asp Ser Leu Lys G u 195 Thr Phe Leu Phe Al a Asp Sy Sy 70 Sy Lys Leu Asp Lys 150 Val G u Ser G n Lys Ar g 55 Leu Leu Al a Al a Al a 135 Ser Thr G y lie G u 25 Hi s Ser Phe G u Gy 105 Leu Thr Hi s SIle Ser 185 G u Leu Val Val Val Asp Asp Ar g Lys Ser Al a 155 Asp SIle Tyr Al a Tyr Asn I le G n SIle G u Cys 140 Leu Ar g Hi s Sy Ar g G u Tyr G n Asp Gy Hi s 125 Asp G u Val Phe Val Al a Ser Tr p G u et SIle 110 Asn SIle Al a Ar g Thr 190 Leu Val Leu Asn Val Val Leu Ser Sy Leu Asn 175 Sy <210> 439 <211> 1039 <212> DNA <213> Corynebacter i um gl ut ai cum <220> <221> CDS <222> (101)..(1039) <223> RXA02351 <400> 439 tgacacttta cagactggtt ttcaactaat gacaccgaaa gaaatacacc tcaacctttt tgctttcggt gccgggcacc acgcggcggc gtggcgagcg gtg gag gga agc gtc 115 Val G u Gy Ser Val 1 gaa aag ctg ggt tta att tcc tgg tgg gag gaa ctc gcg cgc acc gct 163 Su Lys Leu y Leu I e Ser Trp Trp u u Leu Ala Arg Thr Ala 15 Page 519 gag cgg G u Ar g aat ccg Asn Pro acc gcg Thr Al a agc aca Ser Thr atc gcc I e Al a gtg aca Val Thr ttg ccg Leu Pro acc at c Thr Ile 135 gat cgt Asp Arg 150 cat gat His Asp cct ccg Pro Pro gga cgg G y Arg tgg gat Trp Asp 215 gcc act Al a Thr 230 act ttt Thr Phe ctt aat Leu Asn ttt gtg Phe Val ggc aag G y Lys gtc ggt Val G y ttg act Leu Thr att tcc Ile Ser agc ttg Ser Leu tcg atg Ser Vbet 105 ggt cac G y Hi s 120 act gcg Thr Al a gct gga Al a G y ggt gag G y G u cag ggt G n Gy 185 gaa at c G u Ile 200 ttg gag Leu G u gcc cag Al a n gtt ggc Val G y gcg ttg Al a Leu 265 ggt caa G2y n 280 seql i st cor r ect ed. t xt ctg gat gcg gtc ttt ttg gcc gat ggg cag gcg att Leu Asp Ala Val Phe Leu Ala Asp Gy n Ala lie ct g Leu gcg Al a agt Ser gat Asp acc Thr gat Asp ct g Leu aaa Lys ttc Phe 170 cga Ar g gct Al a caa G n ggt Sy acg Thr 250 ct g Leu gat Asp gag G u at g bet acg Thr 75 cat Hi s gat Asp gtt Val tgg Tr p ttt Phe 155 tt c Phe ccc Pr o gcg Al a gcg Al a cgc Ar g 235 acc Thr ccg Pr o tgc Cys aat Asn gcg Al a 60 ttt Phe at t SIle gcg Al a cgc Ar g gat Asp 140 gcg Al a caa G n gt a Val aaa Lys caa G n 220 gag G u gt g Val gt c Val tcg Ser ggg Gy 45 cgg Ar g tgg Tr p tcg Ser gag G u t at Tyr 125 tct Ser gac Asp gt c Val ctt Leu t ac Tyr 205 gat Asp ccc Pr o gaa G u aaa Lys acg Thr 285 ccg Pr o gcg Al a cag G n ggt G y gcg Al a 110 gcg Al a tgg Tr p t cc Ser gct Al a ttt Phe 190 gcc Al a t at Tyr at g Met gaa G u gac Asp 270 tgg Tr p ggc Gy acg Thr ccg Pr o ggg G y 95 cgt Ar g cgc Ar g cct Pr o tcg Ser ggt Sy 175 cag G n gag G u cgc Ar g ccg Pr o gcg Al a 255 t ca Ser gat Asp tgg Tr p aac Asn ttt Phe 80 cgt Ar g aac Asn gct Al a gcg Al a ct c Leu 160 ccg Pr o gct Al a gca Al a tct Ser gt g Val 240 cgt Ar g ct a Leu ttg Leu ttt Phe aat Asn cat Hi s gct Al a cac Hi s gcg Al a gaa G u 145 at t SIle ct g Leu gga Sy at t l Ie gat Asp 225 ctt Leu gca Al a aat Asn gat Asp ttg Leu at t SIle gcg Al a gga G y ggg G y gaa Gu 130 agt Ser aaa Lys aat Asn t cc Ser t ac Tyr 210 at t SIle cct Pr o aaa Lys cag G n gca Al a 290 gag G u ggg G y gcg Al a at c SIle at g Met 115 ttt Phe ttg Leu tct Ser at c SIle t ca Ser 195 tct Ser cat Hi s ggt G y cag G n ttg Leu 275 cct Pr o ccg Pr o ttg Leu cgg Ar g aat Asn 100 gat Asp at t SIle gt g Val at c SIle ccc Pr o 180 ccg Pr o gt g Val gct Al a ttg Leu cag G n 260 agt Ser ccc Pr o gtg Val at c SIe at g Met gtg Val gcg Al a gaa Gu at g Met gat Asp 165 agt Ser caa G n gcg Al a cgt Ar g gtg Val 245 gct Al a ttc Phe cca Pr o Page 520 seql i st cor r ect ed. t xt cca ctg cca ccg cta gaa gag ttt tcc ggt cct aaa ggc agg tac gaa Pro Leu Pro Pro Leu Gu u Phe Ser Gy Pro Lys Gy Arg Tyr u 295 300 305 acg gtc ctg cgg Thr Val Leu Arg 310 <210> 440 <211> 313 <212> PRT <213> Corynebacter i um gl ut ai cum 1027 1039 <400> 440 Val G u Gy Ser 1 Leu Ala Arg Thr Asp G y G n Al a Phe Leu G u Pro Asn Ile G y Leu His Ala Ala Ar g Al a G y Ile Asn 100 Hi s G y IVet Asp 115 Ala G u Phe Ile 130 G u Ser Leu Val 145 Ile Lys Ser Ile Leu Asn lie Pro 180 Sy Ser Ser Pro 195 Ile Tyr Ser Val 210 Asp Ile His Ala 225 Leu Pro y Leu Al a Lys G n G n 260 Asn G n Leu Ser 275 Val 5 Al a l Ie Val SIle Met Val Al a G u Met Asp 165 Ser G n Al a Ar g Val 245 Al a Phe u G u Asn Thr Ser 70 I e Val Leu Thr Asp 150 Hi s Pr o Sy Tr p Al a 230 Thr Leu Phe Lys Ar g Pr o Al a 55 Thr Al a Thr Pr o SIle 135 Ar g Asp Pr o Ar g Asp 215 Thr Phe Asn Val Leu Sy Val 40 Leu SIle Ser Ser Sy 120 Thr Al a Sy G n G u 200 Leu Al a Val Al a Gy 280 Sy Lys 25 Sy Thr Ser Leu bet 105 Hi s Al a Sy G u Sy 185 I le G u G n Sy Leu 265 G n Leu 10 Leu Leu Al a Ser Asp 90 Thr Asp Leu Lys Phe 170 Ar g Al a G n Sy Thr 250 Leu SIle Asp G u Met Thr 75 Hi s Asp Val Tr p Phe 155 Phe Pr o Al a Al a Ar g 235 Thr Pr o Ser Al a Asn Al a Phe SIle Al a Ar g Asp 140 Al a G n Val Lys G n 220 G u Val Val Tr p Val Sy Ar g Tr p Ser G u Tyr 125 Ser Asp Val Leu Tyr 205 Asp Pr o G u Lys Tr p Phe Pr o Al a G n Sy Al a 110 Al a Tr p Ser Al a Phe 190 Al a Tyr Met G u Asp 270 G u Leu Sy Thr Pr o Sy Ar g Ar g Pr o Ser Sy 175 G n G u Ar g Pr o Al a 255 Ser G u Al a Tr p Asn Phe Ar g Asn Al a Al a Leu 160 Pr o Al a Al a Ser Val 240 Ar g Leu Asp Cys Ser Thr Trp Asp Leu 285 Page 521 seql i st cor r ect ed. t xt Asp Ala Pro Pro Pro Pro Leu Pro Pro Leu G u u Phe Ser Gy Pro 290 295 300 C) Lys y Arg Tyr G u Thr Val Leu Arg 1) 305 310 <210> 441 <211> 18 <212> DNA <213> Arti fi ci al Sequence C1 <220> 0 <223> Description of Artificial Sequence: prirrer 00 0 <400> 441 Sggaaacagta tgaccatg 18 (N 0 <210> 442 0 <211> 17 C1 <212> DNA <213> Arti fi ci al Sequence <220> <223> Descri pti on of Artifi ci al Sequence: pr i rrer <400> 442 gtaaaacgac ggccagt 17 Page 522